LOCUS       DALAGD010000001        81166 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.001, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000001 DALAGD010000000
VERSION     DALAGD010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 81166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81166
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.001"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(232..1401)
                     /locus_tag="O2701_000001"
     CDS             complement(232..1401)
                     /locus_tag="O2701_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol--glycerophosphate
                     glycerophosphotransferase"
                     /protein_id="HCZ4291921.1"
                     /translation="MIKNTIKKLIEHSIYTTFKLLSKLPNKNLIYFESFHGKQYSDNP
                     KALYEYLTEHSDAQLIWGVKKGYEHIFQQHNVPYVTKFSMKWFLAMPRAKAWMINTRT
                     PDWLYKSPRTTYLQTWHGTPLKKIGLDISNVKMLGTNTQNYQDGFKKESQRWDYLVSP
                     NPYSTSIFQNAFHVSRDKILETGYPRNDKLSHKRNDTEYINGIKTRLNIPLDKKVIMY
                     APTWRDDEAIREGSYQFNVNFDIEALRQALDDDYVILLRMHYLVVTRIDEHDDFVKDV
                     SDYEDISDLYLISDALVTDYSSVMFDFGVLKRPQIFYAYDLDKYGDELRGFYMDYKKE
                     LPGPIVENQTALIDALKQIDETANEYIEARTVFYQKFCSLEDGQASQRICQTIFK"
     gene            complement(1829..3523)
                     /locus_tag="O2701_000002"
     CDS             complement(1829..3523)
                     /locus_tag="O2701_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCZ4291922.1"
                     /translation="MTKTKQAIHIDNIYWERVQLYIEGHSEGVDLTSGQFVLRNLTET
                     KTLEANEMKIDGNTFICRFNVAILDDGYYLPMDKYLFVYHDQLEYIGQLNPNIIDQAY
                     AALNEEQIEEYNELTTQNGKVNYLLAYDAKVFRKGGVSQHTVYTITPEIASDVNEFVF
                     DIEITLPQEKSGVIATSAHWLHKQGHKASFESRSFLFKAIFNITKLLHIKRSKTILFT
                     SDSRPNLSGNFKYVYDELLRQKVDFDYDIKTVFKENITDRRKWRDKFRLPYLLGKADY
                     IFVDDFHPLIYTVRFRPSQEIIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKA
                     YVSSETDIPFYAEAFGIREENVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKV
                     ILFAPTFRGNGHGTAHYPFFKIDFERLARYCEKHNAVVLFKMHPFVKNRLNISREHRQ
                     YFIDVSDHREVNDILFVTDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEP
                     FESFVPGKIVQSFDALMDALDNEDYEVEKVVPFLDKHFKYQDGRSSERLVKDLFRR"
     gene            complement(3545..4570)
                     /locus_tag="O2701_000003"
     CDS             complement(3545..4570)
                     /locus_tag="O2701_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCZ4291923.1"
                     /translation="MINQVYQLVAPRQFDVTYNNVDIYGNHVIVRPLYLSICAADQRY
                     YTGRRDENVLRKKLPMSLVHEAVGEVVFDSKGVFEKGTKVVMVPNTPTEQHHIIAENY
                     LASSYFRSSGYDGFMQDYVVMAHDRIVPLPNDIDLSTISYTELVSVSYHAIQRFERKS
                     IPLKTSFGIWGDGNLGYITAILLRKLYPEAKTYVFGKTDYKLSHFSFVDDIFTVNQIP
                     DDLKIDHAFECVGGKGSQVALQQIVEHISPEGSIALLGVSELPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFEQVVDLYRKYPDIVEKLALLKGHEINVCTMQDIVQAFEMDLSTSWGK
                     TVLKWTI"
     gene            complement(4563..5279)
                     /locus_tag="O2701_000004"
     CDS             complement(4563..5279)
                     /locus_tag="O2701_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCZ4291924.1"
                     /translation="MIYAGILAGGIGSRMGNVPLPKQFLDIDNKPILIHTIEKFILVS
                     EFNEIIIATPAQWISHTQDILKKYNITDQRVKVVAGGTDRNETIMNIIDHIRNVNGIN
                     NDDVIVTHDAVRPFLTQRIIKENIEVAAKYGAVDTVIEAIDTIVMSKDKQNIHSIPVR
                     NEMYQGQTPQSFNIKLLQDSYRALSSEQKEILSDACKIIVESGHAVKLVRGELYNIKV
                     TTPYDLKVANAIIQGDIADD"
     gene            complement(5807..6850)
                     /locus_tag="O2701_000005"
     CDS             complement(5807..6850)
                     /locus_tag="O2701_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCZ4291925.1"
                     /translation="MKALKLYGVEDLRYEDNEKPVIESANDVIIKVRATGICGSDTSR
                     YKKMGPYIKGMPFGHEFSGVVDAIGSDVTHVNVGDKVTGCPAIPCYQCEYCLKGEYAR
                     CEKLFVIGSYEPGSFAEYVKLPAQNVLKVPDNVDYIEAAMVEPSAVVAHGFYKSNIQP
                     GMTVAVMGCGSIGLLAIQWARIFGAAHIIAIDIDAHKLDIATSLGAHQTINSKEENLE
                     KFIENHYANQIDLAIESSGAKVTIGQILTLPKKGGEVVLLGIPYDDIEIDRVHFEKIL
                     RNELTVCGSWNCLSSNFPGKEWTATLHYMKTKDINVKPIISHFLPLEKGPETFDKLVN
                     KKERFDKVMFTIY"
     gene            complement(6874..7020)
                     /locus_tag="O2701_000006"
     CDS             complement(6874..7020)
                     /locus_tag="O2701_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4291926.1"
                     /translation="MVESMLTFMLGPLRQITDFYMEHLLVSNSIVIAGYFATGIFKKK
                     KVVN"
     gene            complement(7022..8077)
                     /locus_tag="O2701_000007"
     CDS             complement(7022..8077)
                     /locus_tag="O2701_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCZ4291927.1"
                     /translation="MKALVKTREGHGNLELLDKEVATPLDDKVKIKVHYAGICGTDIH
                     TYEGHYKVNFPVTLGHEFSGEIVEVGADVKDFKVGDRVTSETTFYVCNECEYCKSKDY
                     NLCNHRKGIGTQVDGAFTNYVIAREESLHHIPDEVSYQSAAMTEPLACAYHGVSKIQV
                     NSGDVAVVMGPGPIGLLVAQVLKSKGATVVVTGLDNDKVRLDKAEALHMDYVVNLQQT
                     DLKTYINGITDGYGADVVVECSGAVPAARQGLDILRKKGFYSQIGIFKDAEIPFDMEK
                     VIQKEITVVGSRSQKPADWEPSLQLMADGLVNAEALVTKIYDISKWDEAYQHLKSGEG
                     IKVLLKPLDLDENEGEN"
     gene            complement(8095..9354)
                     /locus_tag="O2701_000008"
     CDS             complement(8095..9354)
                     /locus_tag="O2701_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCZ4291928.1"
                     /translation="MSYFTDFVRGFLDLGATVILPVVIFLLGLFFRQKIGAAFRSGLT
                     IGVAFVGIFLVIDLLVKNLGPAAQAMVKNLGVSLNVIDVGWPATSSIAWASSVAAFII
                     PLGIIVNVVLLVTKVTKTMNVDIWNFWHYTFTAAMVYAVSGSIWQALLAAVIFQIICL
                     KVADWTAPMMSEFFDLPGVSIATGSTISYAPGIYLVKLLQKVPGLNKLDADPETIQKR
                     FGAFGESIFVGLILGLGIGVLAGYKPGDIINLGMSMAAVMVLMPRMVKILMEGLMPVS
                     ESARTWLNKRFGEREIYIGLDAAVALGHPAVISTALILVPITVLLAVILPGNQVLPFG
                     DLATIPFVVAFIVGAARGNIIHSVIVGTIMIAISLYIATDVAPIFTDMAKGTNVQMPK
                     GSSEISSIDQGGNIVNYLIFKLFSLFN"
     gene            complement(9581..9859)
                     /locus_tag="O2701_000009"
     CDS             complement(9581..9859)
                     /locus_tag="O2701_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCZ4291929.1"
                     /translation="MKQVLVACGAGIATSTVVNNAIEEMAKEHNIKVDIKQIKITEVG
                     PYEDTADLLVTTAMTKKEYKFPVINARNFLTGIGIEETKQQILTELQK"
     gene            complement(9882..10349)
                     /locus_tag="O2701_000010"
     CDS             complement(9882..10349)
                     /locus_tag="O2701_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCZ4291930.1"
                     /translation="MGQQLVHKENIMLNLSATDKESVLSQMSDVLFQNGFVKSTFKDA
                     VIDREKEFATGLPTHLCSVAIPHTDVEHINHRTIGVAVLEKEVPFIEMGTLDQQTEVK
                     IVFMLAMDKVDDQLKLLQQLMQIFQSEEKLEQILRTKDETILATLINDYLEYN"
     gene            complement(10334..12430)
                     /locus_tag="O2701_000011"
     CDS             complement(10334..12430)
                     /locus_tag="O2701_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCZ4291931.1"
                     /translation="MNGDNQQILREIVLNPTIHGKELESIFGLSRRQLGYRIQKINLW
                     LEQEGYPKLERTSQGNFIVSSEIMTLFKRDVSEQQMLNGNNVIFSIETRRYYLMLMLF
                     SKENAMSLNHFSIDLQVSKNTVIHDINHVKEQLENHGLSLKYSRKHGYEIVGDEFEVR
                     RFFIKLIDQRLNHDITKSEVLKALNLTFEDIAYQKDKIKQVEQFLKSRFIDKSLSSLP
                     YVLCVIRRRIQSGHVMNPLNINYQYLRDTKEYQATEIMTQHEPDLPEAEKLYLTLHLL
                     STSVQWTDLQESDSISNLTMAIAQMIHHFEQITFINIEDKEKLSQQLLLHLTPAFYRI
                     KYNLTDRDELINPLQGNYQSLFHMVKQSCQSLTEYFGKSLPDNEIAYLTMLFGGSLRR
                     QDENFDGKIKAIIVCTQGTSVSQMMLYELRNLFPEIIFLDAISLRTFENYTLDYDIVF
                     SPMFVLTHKKLFITKVALSENEQRKLRKEVMKYINKESADIDKEINKLMALIERTTTV
                     NDITELRDGLEDFIANYNSISTINGSIVTQNKTLDLADLIPARHVKRMHHVENIDEAI
                     AKASDVLVANHFIDIKYIHEMQQVFDDSYMVIMQNIAIPHAYSEKHVHKTAMSMLILQ
                     EPIYMSDGTAIHIIVPIAAVDKVTHLRALLQLRDVAQDNDAIKRIIQSRKNSDVNEIL
                     KNYSNKEARENGWDSN"
     gene            complement(12561..12659)
                     /locus_tag="O2701_000012"
     CDS             complement(12561..12659)
                     /locus_tag="O2701_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4291932.1"
                     /translation="MYVKNASKKHKTQKYMQKFQLLYFKRLKSQKI"
     gene            complement(12769..13704)
                     /locus_tag="O2701_000013"
     CDS             complement(12769..13704)
                     /locus_tag="O2701_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCZ4291933.1"
                     /translation="MKRKIIMDCDPGHDDAIALILAGAIDSPLEILAVTTVAGNQSVD
                     KNTTNALNVLDIMGRQDIAVAKGADRPLIKPAAFASEIHGESGLDGPKLPSTPSRQAV
                     AMPASDVIINKVMTSDTPVTIVATGPLTNVATALIREPRIAEHIESITLMGGGTFGNW
                     TPTAEFNIWVDAEAAKRVFESGITINVFGLDVTHQVLADDHVIERFESINNPVAQFVV
                     ELLQFFKKTYKTHFNMDGGPIHDACTILYLLQPELFTMVPVNIDIEHQSPLTYGTMAV
                     DLNHVTGKPANAYFATAVDVEEVWNLIDHKLRTYE"
     gene            14066..15595
                     /locus_tag="O2701_000014"
     CDS             14066..15595
                     /locus_tag="O2701_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCZ4291934.1"
                     /translation="MKSLFEKAQQFGKSFMLPIAILPAAGLLLGIGGALSNPNTVKAY
                     PILDITLLQNIFTLMSAAGSIVFQNLPVIFAIGVAIGLSRSDKGTAGLAALLGFLIMN
                     ATMNGLLTITGTLAKDQLAQNGQGMVLGIQTVETGVFGGIITGIMTAILHNKYHKVVL
                     PPYLGFFGGSRFVPIVTAFAAIFLGVLMFFIWPSIQAGIYHVGGFVTKTGAIGTFVYG
                     FILRLLGPLGLHHIFYLPFWQTALGGTLEVKGHLVQGTQNIFFAQLGDPDVTKYYSGV
                     SRFMSGRFITMMFGLCGAALAIYHTAKPEHKKVVGGLMLSAALTSFLTGITEPLEFSF
                     LFVAPILYVIHAFFDGLAFMMADIFNITIGQTFSGGFIDFLLFGVLQGNSKTNYLYVI
                     PIGIVWFCLYYIVFRFLITKFNFKTPGREDKAAAQQVEATERAQTIVAGLGGKDNIEI
                     VDCCATRLRVTLHQNDKVDKVLLESTGAKGVIQQGTGVQVIYGPHVTVIKNEIEELLG
                     D"
     gene            complement(15915..16868)
                     /locus_tag="O2701_000015"
     CDS             complement(15915..16868)
                     /locus_tag="O2701_000015"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCZ4291935.1"
                     /translation="MNKFKGNKVVLIGNGAVGSSYAFSLVNQSIVDELVIIDLDTEKV
                     RGDVMDLKHATPYSPTTVRVKAGEYSDCHDADLVVICAGAAQKPGETRLDLVSKNLKI
                     FKSIVGEVMASKFDGIFLVATNPVDILAYATWKFSGLPKERVIGSGTILDSARFRLLL
                     SEAFDVAPRSVDAQIIGEHGDTELPVWSHANIAGQPLKTLLEQRPEGKAQIEQIFVQT
                     RDAAYDIIQAKGATYYGVAMGLARITEAIFRNEDAVLTVSALLEGEYEEEDVYIGVPA
                     VINRNGIRNVVEIPLNDEEQSKFAHSAKTLKDIMAEAEELK"
     gene            complement(17179..17286)
                     /locus_tag="O2701_000016"
     CDS             complement(17179..17286)
                     /locus_tag="O2701_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4291936.1"
                     /translation="MSVCTPTIERIYLTNSKNIVVFLEKSKHDNKNVKV"
     gene            17441..18586
                     /locus_tag="O2701_000017"
     CDS             17441..18586
                     /locus_tag="O2701_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide dioxygenase"
                     /protein_id="HCZ4291937.1"
                     /translation="MLTEQEKDIIKQTVPLLKEKGTEITSIFYPKMFKAHPELLNMFN
                     QTNQKRGMQSSALAQAVMAAAVNIDNLSVIKPVIMPVAYKHCALQVYAEHYPIVGKNL
                     LKAIQDVTGLEENDPVIQAWAKAYGVIADVFIQIEKEIYDQMMWIGFKPFKITNIKQE
                     SEDIKSFTVETEEYDFSEFTPGQYITVDVSSDKLPYRAKRHYSIVSGEKNHLTFGVKR
                     DVTTEHEGEVSTILHDEIKEGDMINLAAPVGGFVLENTTEPQLFLGSGIGVTPLVAMY
                     EAASAKGLDTQMVQVAENEQHLPFKDNFNSIASHHDNAKLYTHLKDKQGYIGAEELQV
                     FLANKPEIYICGGTKFLQSMIEALKSLNYDMDRVHYETFIPRLSVAV"
     gene            18612..18785
                     /locus_tag="O2701_000018"
     CDS             18612..18785
                     /locus_tag="O2701_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4291938.1"
                     /translation="MKLNQRYVKVFALYFVSIVTANIIVKNNNLIKTLIQTIAGYTVF
                     AVGLKYLTKRKNK"
     gene            18942..19298
                     /locus_tag="O2701_000019"
     CDS             18942..19298
                     /locus_tag="O2701_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCZ4291939.1"
                     /translation="MTVDIGRIYDNKDNTDAIRILVDRVWPRGISKRTANLDYWLKDI
                     APSTELRQWFQHDPKLFGAFKEKYEKELRDQDAQKDAFEKLKDIVNQHNHVLLLYAAK
                     DTKHNQAVVLQQLLNT"
     gene            19496..20971
                     /locus_tag="O2701_000020"
     CDS             19496..20971
                     /locus_tag="O2701_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCZ4291940.1"
                     /translation="MKFKRLATIFSAVLVLSGCGSMHSSGKDLNISLPLKTKSIAPYE
                     TDVPVKIGAAESLFKTNDQGKIEKALVKSYHQPNDTTLDIELKDNIKFQNGQKLTAEK
                     VKSSLENSMKKSDLVKYSLPISSITAKGQKLTIKTNSAYPELVSELANPFMAIYDTDA
                     KSDVNQTPVGTGPYQIKDYKQSRKISLSNFKDYWQGKPKLDHITVTYQEDGNNRVRNL
                     ESQKDDLITDVPVNKVQDIENNQNLKVSKESGFRTSLLMYNHTNKKMTKSVREALDHI
                     IDRQGIADHIYQGYAKPATSPFNDKIPYIKEPKLTKQNIEQAKMLLAKDGYTKEHPLK
                     IKLITYDGRPELSKIAQVLQSDAKKANIEIDIKSVDDIEGYLKDRSAWDATMYSFGTI
                     PRGDTGYFFNQAYKKDGAINKGDYNNSNVDDLINQLNHTVDVKERHNISNDIIKLSSR
                     DVPNSYIAYNDQIVAANSKVKNYKVTPEGIYLIDYRTTIER"
     gene            complement(21285..22427)
                     /locus_tag="O2701_000021"
     CDS             complement(21285..22427)
                     /locus_tag="O2701_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HCZ4291941.1"
                     /translation="MSSASTQSTKTSDIHNESIDKQMEAKAHETAQNTDLKNEARSLF
                     DNATKSIGRLAGNDESLNLNLKDMLSEVFKPHTKNEADEIFIAGTAKTTPAICDISEE
                     WGKPWLFSRVFIAFTVTFIGLWVMAAIFNNTNAIPGLIFIGALTVPLSGLFFFYESNA
                     FKNISIFEVIIMFFIGGVFSLLSTMVLYRFVVFSDQFERFGSLTFFDAFLVGLVEETG
                     KALIIVYFVNKLKTNKILNGLLIGAAIGAGFAVFESAGYILNFALGENVPLLDIVFTR
                     AWTAIGGHLVWSAIVGAAIVIAKEQHGFEFKDIFDKRFLIFFLSAVVLHGIWDTSLTV
                     LGSDTLKIFILIVIVWILVFILMGAGLKQVNLLQKEFKEQQKKVDE"
     gene            22868..24445
                     /locus_tag="O2701_000022"
     CDS             22868..24445
                     /locus_tag="O2701_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HCZ4291942.1"
                     /translation="MEVTCLKQITWHDLQHIIKDGDVIGLPALAVANLPAEVLRAVLA
                     QHDTYHTPNDLTFILANDIHSLGAAPDLDDFIERRMIKRVIMSILTASSKTAQAMKNN
                     DIEAYFLPQGIIATHYRQSNQLLPGVITKIGLNTAVDPRYGGGKVNTRTTDDLVSLVT
                     INDETYLHYTFPSVDVALLRGTYADQQGNIYLTQEAYLSECYHVALNAKANHGKVIVQ
                     VKALVDDYQLKPNEVVIPGNLVDYVYVTEDEKNHRQVIQSHYLPALSGEERIDGIPEP
                     ALPFNSRKLILRRAAQFLTYGDTISIGYGINNELSNLLHEECVEHDVQPILDVGIFGG
                     FVGSREHFGMNYNADVRMPHDRAWDFIYNNGVSVAYLSFAEVDQYGNVNVSYFNDRLN
                     GCGGFIDITQSVNKIIFSGTFVAGSHVSCHNQRLNIETEGQNQKFVSDVSHIDFNAQY
                     SQSLEQEIYFVTDRAVFELTNQGLKLIEIAPGLDLHKDILNQMAFKPIIADHLKLIDT
                     SIYKEKWGQLKQSIHKV"
     gene            24471..25976
                     /locus_tag="O2701_000023"
     CDS             24471..25976
                     /locus_tag="O2701_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HCZ4291943.1"
                     /translation="MNFDWIKTRSDFDDDKPAVIDHAKQTSWTYQQLNARADNMAHYL
                     TSQGVKKGDVIGIFAPNDIAILDLLFACFKTGAVFLPLNWRLNPKEIAAIVEDAQLKL
                     LFYAEKHLSSLTDIDQNLLHMDIDVAQYDEIVNPDYHQPFQATPVEPQDLAALIYTSG
                     TTGSPKGVMFSYESFVHNGANLELTYKFNSNYITIVSTPMFHVLGFNDTVLPVLMSGG
                     TLILQRYFNGEELNDMIAQYHPTFIIMIPTMYYSTLRASNFNPENFRAMDYIIQGGSQ
                     PLPSIQAAFKQYGINIINGYGLTEAPLVLVNTPENSKRKPMSIGKAVMFVDARILDDN
                     GEEVPTGEIGELAIKAKNVTPGYWNKPAETAKAFHGRYLLTGDLAKMDNDGDIFIIDR
                     KKELIITGGENVLPSEVENALAEHPLVDRCVVVGYDHPKYGESIAAAIILREDEPHYA
                     EILNQHMRSRLAGYKVPRMYVPVTHMPLNSTQKPDKLAIRQMMNDKVSQTL"
     gene            26088..27299
                     /locus_tag="O2701_000024"
     CDS             26088..27299
                     /locus_tag="O2701_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HCZ4291944.1"
                     /translation="MTFEKETVLKTLFPEDVLSIAKGLTDGEVEFLQQVDSLLESKYR
                     ENINQHWIDATVPEDYFKDLGELNYFNNPLLYKDRPNAKMPSQLFQFFMSYLLARFDI
                     SLATLLGVHQGLGHNTFYFGGSKEQIAKYVPKLQSHELRTCFALTEPEHGSDVAGGLE
                     TVAERQGDTWVINGEKKWIGGAHVSDVIPVFAVNKETGKPHCFVVRPEQDGVDIEVID
                     NKIALRIVPNALIKLTNVKVDEADRLQNITSFKDIAKILYSTRAGVAYMATGGMAGAL
                     RATLDYVTERKQFGKPISKYQLIQEKLAMMQGNLAQAMATCAQLANMQAHGEYDEVAT
                     STAKMMNALRLRETVAMGRGITGGNGILADDYDIARFFSDAEAIYTYEGTHEINALVI
                     GRALTGDSAFV"
     gene            27485..29746
                     /locus_tag="O2701_000025"
     CDS             27485..29746
                     /locus_tag="O2701_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HCZ4291945.1"
                     /translation="MTINKVTVLGAGTMGAQLAALFVNAGLKVKLLDIVVDKNDPNLI
                     AKKSYDKITDKKRPLLFDLNLASHLTYGNFDDDLVNDDADLYIEAVKEDIEIKHAVWQ
                     QVLQHAKEDALFATNTSGIPINAIAQAFNEKDQERFFGLHFFNPPRIMKLVELIPTSH
                     TKESIILDVKNFAQNVLGKGVIVVNDVPGFVANRVGTQTMNDIMYRAEQHKISIVDVD
                     ALTGQAIGRPKTGTYALSDLVGLDIAVSVIKGMQQVPEETPYFHDVKIVNTLFDNGAL
                     GRKTKQGFYKKDKETKARLVYDVEKQDYVPVSQPQLPILNEFNKDLVHNLDTIFNAQD
                     EAGLFLWETLRNNFYYSAINVPKATDDFRDIDRALVWGFNWKLGPFQLWDAMGYERVK
                     TRMEDELGDLPQWISDLDGGFYKQDETIEYATPISHFVKDELWDKGDAKLSVTHDDQL
                     LLKLQSKNNVITDEFNDALVDAIDLLENDHYTSMVIYADGNNFSVGANLFLMKKAHED
                     GLVDDVVAQSIDKLHYSFNRLKYSLKPVVTAVQGRALGGGCELVLYSPIVVAASETYI
                     GLVEAGVGLLPSGGGLAEMADRILRTSHKFDDKQASMTKVLTNIAFAKVSTNAFEARR
                     YGYLRDTDTIIFNTAQRVEVALKRAKYEAETNYIPNPRHQYIALGEDFKALIQGQLDA
                     QRRGHFISDHDYHIALNIATILAGGDLPRNTFINQRYIQSLEKIGFIDLLKSKKSYER
                     IAHMLKTGKPLRN"
     gene            29776..30960
                     /locus_tag="O2701_000026"
     CDS             29776..30960
                     /locus_tag="O2701_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HCZ4291946.1"
                     /translation="MQEAYIVAYGRSAAAKAKQGALFHERPDDVAAKVLQGVLKRIDG
                     KFNKNMIEDVIVGTAFPEGLQGQNIARTIALRAGLSDTVPGQTVNRYCSSGLQTIAIA
                     ANQIMAGQGDILVAGGVELMSAVPMGGNEPTNNPTLQYDDIGASYPMGLTAENVASQF
                     DVSREDQDAYAVRSHQRAYDAQRDGRFKDEIIPIQVNSVEYTNAGPKVHTNIFDQDEF
                     IRPDTTMEALAKLRTVFKADGTVTAGTSAPLSDGAGFVVLMSGDKVKELGVTPIARFV
                     GFKAVGVDPKIMGIGPAYAIPEVLSLSNLSVEDIDLIELNEAFASQTIASIKEVGLDI
                     SRTNVNGGAIALGHPLGATGAMLTARLLNEMGRRPDSRYGMVTMCIGVGMGAAAIFEY
                     VR"
     gene            31274..31381
                     /locus_tag="O2701_000027"
     CDS             31274..31381
                     /locus_tag="O2701_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4291947.1"
                     /translation="MCVRTRLVSSSSARLSKAIIIAVIVVYHLDVRGLF"
     gene            complement(31563..33392)
                     /gene="coa"
                     /locus_tag="O2701_000028"
     CDS             complement(31563..33392)
                     /gene="coa"
                     /locus_tag="O2701_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylocoagulase"
                     /protein_id="HCZ4291948.1"
                     /translation="MKKQIISLGALAVASSLFTWDNKADAIVTKDYSGKSQVNAGSKN
                     GTLIDSRYLNSALYYLEDYIIYAIGLTNKYEYGDNIYKEAKDRLLEKVLREDQYLLER
                     KKSQYEDYKQWYANYKKENPRTDLKMANFHKYNLEELSMKEYNELQDALKRALDDFHR
                     EVKDIKDKNSDLKTFNAAEEDKATKEVYDLVSEIDTLVVSYYGDKDYGEHAKELRAKL
                     DLILGDTDNPHKITNERIKKEMIDDLNSIIDDFFMETKQNRPKSITKYNPTTHNYKTN
                     SDNKPNFDKLVEETKKAVKEADDSWKKKTVKKYGETETKSPVVKEEKKVEEPQAPKVD
                     NQQEVKTTAGKAEETTQPVAQPLVKIPQGTITGEIVKGPEYPTMENKTVQGEIVQGPD
                     FLTMEQSGPSLSNNYTNPPLTNPILEGLEGSSSKLEIKPQGTESTLKGTQGESSDIEV
                     KPQATETTEASQYGPRPQFNKTPKYVKYRDAGTGIREYNDGTFGYEARPRFNKPSETN
                     AYNVTTHANGQVSYGARPTQNKPSKTNAYNVTTHGNGQVSYGARPTQNKPSKTNAYNV
                     TTHANGQVSYGARPTYKKPSKTNAYNVTTHADGTATYGPRVTK"
     gene            33582..33926
                     /locus_tag="O2701_000029"
     CDS             33582..33926
                     /locus_tag="O2701_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN family protein"
                     /protein_id="HCZ4291949.1"
                     /translation="MTTQMKIKTYLVAGIKAALLDTTGIKLASKSETTSHTYQHQALV
                     DQLHELIANTDLNKLSYLNLDAFQKRDILAAHYIAKSAIRTKNLDQMTKAKQRLESIY
                     NSISNPLHSQNN"
     gene            complement(34090..35853)
                     /locus_tag="O2701_000030"
     CDS             complement(34090..35853)
                     /locus_tag="O2701_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HCZ4291950.1"
                     /translation="MKRISKDIWAVFKLLYQNKGRFSINALLLQLIMIFISSTYLILL
                     FNMMLKVAGQSQLTINNWTEIVSHPASVILLIIFILSVAFLIYVEFSLLVYMVYAGFD
                     RQIITFKSIFKNAFVNVRKLIGVPVIFFVIYLMLMIPIANLGLSSVLTKNIYIPKFLT
                     EELMKTTKGIIIYGTFMIAVFILNFKLIFTLPLTILNRQSLFKNMRLSWQITKRNKFR
                     LVIEIVILELIIGAILTLIISGATYLAICVDEEGDKFLVSSILFVVLKSALFFYYLFT
                     KLSLISVLVLHLKQENVLDQPGLEFKYPKPKRKSRFFIISMVLAVTCFIGYNMYLLYN
                     NTINTNISIIGHRGFEDKGVENSIPSLKAAAKANVEYVELDTIMTKDKQFVVSHDNNL
                     KRLTGVNKNISESNFKDIVGLKMRQNGHEAKFVSLDEFIETAKQSNVKLLVELKPHGK
                     EPADYTQRVIDILKKHGVEHQYRVMSLDYDVMTKLKKEAPYLKCGYIIPLQFGHFKET
                     SLDFFVIEDFSYSPRLVNQAHLENKEVYTWTINGEEDLTKYLQTNVDGIITDDPALAD
                     QIKEEKKDETYFDRSIRILFE"
     gene            complement(36189..36944)
                     /gene="pflA"
                     /locus_tag="O2701_000031"
     CDS             complement(36189..36944)
                     /gene="pflA"
                     /locus_tag="O2701_000031"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HCZ4291951.1"
                     /translation="MLKGHLHSVESLGTVDGPGLRYILFTQGCLLRCLYCHNPDTWKI
                     SEPSREVTVDEMVNEILPYKPYFDASGGGVTVSGGEPLLQMPFLEKLFAELKENGVHT
                     CLDTSAGCANDTKAFQRHFEELQKHTDLILLDIKHIDNDKHIRLTGKPNTHILNFARK
                     LSDMKQPVWIRHVLVPGYSDDKDDLIKLGEFINSLDNVEKFEILPYHQLGVHKWKTLG
                     IAYELEDVEAPDDEAVKAAYRYVNFKGKIPVEL"
     gene            complement(36967..39216)
                     /gene="pflB"
                     /locus_tag="O2701_000032"
     CDS             complement(36967..39216)
                     /gene="pflB"
                     /locus_tag="O2701_000032"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCZ4291952.1"
                     /translation="MLETNKNHATAWQGFKNGRWNRHVDVREFIQLNYTLYEGNDSFL
                     AGPTEATSKLWEQVMQLSKEERERGGMWDMDTKVASTITSHDAGYLDKDLETIVGVQT
                     EKPFKRSMQPFGGIRMAKAACEAYGYELDEETEKIFTDYRKTHNQGVFDAYSREMLNC
                     RKAGVITGLPDAYGRGRIIGDYRRVALYGVDFLMEEKMHDFNTMSTEMSEDVIRLREE
                     LSEQYRALKELKELGQKYGFDLSRPAENFKEAVQWLYLAYLAAIKEQNGAAMSLGRTS
                     TFLDIYAERDLKAGVITESEVQEIIDHFIMKLRIVKFARTPDYNELFSGDPTWVTESI
                     GGVGIDGRPLVTKNSFRFLHSLDNLGPAPEPNLTVLWSVRLPDNFKTYCAKMSIKTSS
                     IQYENDDIMRESYGDDYGIACCVSAMTIGKQMQFFGARANLAKTLLYAINGGKDEKSG
                     AQVGPNFEGINSEVLEYDEVFKKFDQMMDWLAGVYINSLNVIHYMHDKYSYERIEMAL
                     HDTEIVRTMATGIAGLSVAADSLSAIKYAQVKPIRNEEGLVVDFEIEGDFPKYGNNDD
                     RVDDIAVDLVERFMTKLRSHKTYRDSEHTMSVLTITSNVVYGKKTGNTPDGRKAGEPF
                     APGANPMHGRDQKGALSSLSSVAKIPYDCCKDGISNTFSIVPKSLGKEPEDQNRNLTS
                     MLDGYAMQCGHHLNINVFNRETLIDAMEHPEEYPQLTIRVSGYAVNFIKLTREQQLDV
                     ISRTFHESM"
     gene            39804..40772
                     /locus_tag="O2701_000033"
     CDS             39804..40772
                     /locus_tag="O2701_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCZ4291953.1"
                     /translation="MKSKIYILLLFLIFLSACANTRHSESDKNVLTVYSPYQSNLIRP
                     ILNEFEKQEHVKIEIKHGSTQVLLSNLHNEDFSERGDVFMGGVLSETIDHPEDFVPYQ
                     DTSVTQQLEDYRSNNKYVTSFLLMPTVIVVNSDLQGDIKIRGYQDLLQPILKGKIAYS
                     NPNTTTTGYQHMRAIYSMHHRVSDVHQFQNHAMQLSKTSKVIEDVAKGKYYAGLSYEQ
                     DARTWKNKGYPVSIVYPIEGTMLNVDGIALVKNAHPHPKRKKLVQYLTSRSVQQRLVA
                     EFDAKSIRKDVSEQSDQSIENLKNIPLIPKSKLPDIPHHKFLEMIQ"
     gene            40769..42325
                     /locus_tag="O2701_000034"
     CDS             40769..42325
                     /locus_tag="O2701_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCZ4291954.1"
                     /translation="MTAYKPYRHQLRRSLFASTIFPVFLVIIIGLVSFYAIYIWIEHR
                     TIHQHVDESQSSLHHTEKQIQTFITQHNNSFQELDLTNHHDVTATKRELLKLIHQQPA
                     TLYYELSGPNQFITNNYEHLNTKNMYLFSTHQLKFKNSTYMLKIYMANTPRLSEIKKD
                     NRQFALIVDQYDNILYANDDRFTIGEKYRPQQFGFMNESVKLNHADHRLIIYKDIHEN
                     IEDGITLLIVMAVVLVLLVIFGFISADNMAKRQTKDIETIIQKIYYAKNRHLGTYTPL
                     KNNSELEEINNYIYDLFESNEQLIHSIEHTERRLRDIQLKEIERQFQPHFLFNTMQTI
                     QYLITLSPKLAQTVVQQLSQMLRYSLRTNSHTVELNEELNYIEQYVAIQNIRFDDMIK
                     LHIESSEEARHQTIGKMMLQPLIENAIKHGRDTESLDITIRLTLARQNLHVLVCDNGI
                     GMSSSRLQYVRQSLNNDVFDTKHLGLNHLHNKAMIQYGSHARLHIFSKRNQGTLICYK
                     IPLSRGNVDV"
     gene            42318..43076
                     /locus_tag="O2701_000035"
     CDS             42318..43076
                     /locus_tag="O2701_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCZ4291955.1"
                     /translation="MFKVVICDDERIIREGLKQIIPWGDYHFNTIYTAKDGVEALSLI
                     QQHQPELVITDIRMPRKNGVDLLNDIAHLDCNVIILSSYDDFEYMKAGIQHHVLDYLL
                     KPVDHAQLEVILGRLVRTLLEQQSQNGRSLASCHDAFQPLLKVEYDDYYVNQIVDQIK
                     QSYQTKVTVSDLIQHIDVSESYAMRTFKDHVGITIVDYLNRYRILQSLQLLDRHYKHY
                     EIADKVGFSEYKMFSYHFKKYLQMSPSDYCKQAK"
     gene            complement(43435..44814)
                     /gene="uhpT"
                     /locus_tag="O2701_000036"
     CDS             complement(43435..44814)
                     /gene="uhpT"
                     /locus_tag="O2701_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCZ4291956.1"
                     /translation="MNFFDIHKIPNKGIPLSVQRKLWLRNFMQAFFVVFFVYMAMYLI
                     RNNFKAAQPFLKEEIGLSTLELGYIGLAFSITYGLGKTLLGYFVDGRNTKRIISFLLI
                     LSAITVLIMGFVLSYFGSVMGLLIVLWGLNGVFQSVGGPASYSTISRWAPRTKRGRYL
                     GFWNTSHNIGGAIAGGVALWGANVFFHGNVIGMFIFPSVIALLIGIATLFIGKDDPEE
                     LGWNRAEEIWEEPVDKENIDSQGMTKWEIFKKYILGNPVIWILCVSNVFVYIVRIGID
                     NWAPLYVSEHLHFSKGDAVNTIFYFEIGALVASLLWGYVSDLLKGRRAIVAIGCMFMI
                     TFVVLFYTNATSVMMVNISLFALGALIFGPQLLIGVSLTGFVPKNAISVANGMTGSFA
                     YLFGDSMAKVGLAAIADPTRNGLNIFGYTLSGWTDVFIVFYVALFLGMILLGIVAFYE
                     EKKIRSLKI"
     gene            45047..45541
                     /locus_tag="O2701_000037"
     CDS             45047..45541
                     /locus_tag="O2701_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HCZ4291957.1"
                     /translation="MILSILLIFFCIRLVSLKISINHSKQLKADGAVEYGVKNSKFLA
                     ITHVLIYVLAGVEAFINKDTFSFANGIGLVILIFAYIMLFMVIKTLGGIWTLKLFILP
                     NHPIIKSGLYKITKHPNYFLNIIPELIGVLLLTHATYTTILLVPYAYFLYVRIKQEEK
                     LMNI"
     gene            complement(45903..46871)
                     /locus_tag="O2701_000038"
     CDS             complement(45903..46871)
                     /locus_tag="O2701_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCZ4291958.1"
                     /translation="MKIGVFSVLFYDKNFEDMLDYVSESGLDMIEVGTGGNPGDKFCK
                     LDELLENEDKRQAFMKSITDRGLQISGFSCHNNPISPDPIEAKEADETLRKTIRLANL
                     LDVPVVNTFSGIAGSDDTAKKPNWPVTPWPTAYSEIYDYQWNEKLIPYWQDLAEFAKE
                     QDVKIAIELHAGFLVHTPYTMLKLREATNEYIGANLDPSHLWWQGIDPIAAIRILGQA
                     NAIHHFHAKDTYINQENVNMYGLTDMQPYGNVATRAWTFRTVGYGHSPYVWADIISQL
                     IINGYDYVLSIEHEDPIMSVEEGFQKACQTLKSVNIYDKPADMWWA"
     gene            complement(46926..47966)
                     /locus_tag="O2701_000039"
     CDS             complement(46926..47966)
                     /locus_tag="O2701_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCZ4291959.1"
                     /translation="MTKLKVGVIGVGGIAQDRHIPALLKLKDTVSLVAVQDINTVQMI
                     DVAKRFNIPHAVETPSELFKLVDAVVICTPNKFHADLSIEALNHGVHVLCEKPMAMTT
                     EECDRMIEAANKNHKLLTVAYHYRHTDVAITAKKAIESGVVGKPLVARVQAMRRRKVP
                     GWGVFTNKALQGGGSLIDYGCHLLDLSLWLLGKDMVPHEVLGKTYNQLSKQPNQINDW
                     GTFDHTKFDVDDHVTSYMTFANRASMQFECSWSANIKEDKVHVSLSGEDGGINLFPFE
                     IYEPRFGTIFESKANVEHNEDIAGERQARNFVNACLGIEEIVVKPEEARNVNALIEAI
                     YRSDLDNKSIQL"
     gene            complement(47991..49067)
                     /locus_tag="O2701_000040"
     CDS             complement(47991..49067)
                     /locus_tag="O2701_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCZ4291960.1"
                     /translation="MTIKVGIIGCGGIANGKHMPSLQKVENVEMIAFCDVDISKAASA
                     AEAYGTDNAKVYDDYKALLKDDTIDVIHVCTPNDSHCEITVAGLHAGKHVMCEKPMAK
                     TTAEAQKMIDTAKSTGKKLTIGYQNRFRADSQFLHQAAQRGDLGDIYFGKAHAIRRRA
                     VPTWGVFLDEEAQGGGPLIDIGTHALDLTLWMMDNYEPESVMGSTFHKLNKQHHAANA
                     WGSWNPDEFTVEDSAFGFIKMKNGATIILESAWAINSLEVDEAKCSLSGTKAGADMKD
                     GLRIHGEDMGTLYTKHVELENKGVDFYEGNEVDEAEEEAKAWIDAVVNDTEPVVKPEQ
                     AMVVTKILEAIYQSAKSGKAIYFE"
     gene            complement(49141..49980)
                     /locus_tag="O2701_000041"
     CDS             complement(49141..49980)
                     /locus_tag="O2701_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCZ4291961.1"
                     /translation="MTKKKNILKAIGIYSFIAMMFVIILYPLLWTFGISLNPGTNLYG
                     AKMIPDNATFKNYAFLLFDDSSQYLTWYKNTLIVASANALFSVIFVTLTAYAFSRYRF
                     VGRKYGLITFLILQMFPVLMAMVAIYILLNTIGLLDSLFGLTLVYIGGSIPMNAFLVK
                     GYFDTIPKELDESAKIDGAGHMRIFLQIMLPLAKPILAVVALFNFMGPFMDFILPKIL
                     LRSPEKFTLAVGLFNFINDKYANNFTVFAAGAIMIAVPIAIVFLFLQRYLVSGLTTGA
                     TKG"
     gene            complement(49982..51250)
                     /locus_tag="O2701_000042"
     CDS             complement(49982..51250)
                     /locus_tag="O2701_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCZ4291962.1"
                     /translation="MTKRNPKLAALLSVIPGLGQFYNKRPIKGTIFFIFFISFISVFY
                     SFLNIGFWGLFTLGTVPKLDDSRVLLAQGIISILLVAFAIMLYIINILDAYRNAERFN
                     RNEEIKDPKARMVATWDKTFPYLLISPGTFLLIFVVVFPLIFMFGVAFTNYNLYNAPP
                     RHTLEWVGLDNFKTLFTIGVWRKTFFSVITWTLVWTLVATTLQIALGLFLAIIVNHPV
                     VKGKKFIRTVLILPWAVPSFVTILIFVALFNDEFGAINNDILQPLLGVAPAWLSDPFW
                     AKVALIGIQVWLGFPFVFALFTGVLQSISSDWYEAADMDGASSWQKFRNITFPHVIYA
                     TAPLLIMQYAGNFNNFNLIYLFNKGGPPVSGQNAGSTDILISWVYNLTFEFNNFNMGA
                     VVSLIIGFIVAIVAFIQFRRTSTFKDEGGL"
     gene            complement(51253..52524)
                     /locus_tag="O2701_000043"
     CDS             complement(51253..52524)
                     /locus_tag="O2701_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCZ4291963.1"
                     /translation="MSKILKCITLAVVMLLIVTACGPNRSKEDIDKALNKDNSKDKPN
                     QLTMWVDGDKQMAFYKKITDQYTKKTGIKVKLVNIGQNDQLENISLDAPAGKGPDIFF
                     LAHDNTGSAYLQGLAAEIKLSKDELKGFNKQALKAMNYDNKQLALPAIVETTALFYNK
                     KLVKNAPQTLEEVEANAAKLTDSKKKQYGMLFDAKNFYFNYPFLFGNDDYIFKKNGSE
                     YDIHQLGLNSKHVVKNAERLQKWYDKGYLPKAATHDVMIGLFKEGKVGQFVTGPWNIN
                     EYQETFGKDLGVTTLPTDGGKPMKPFLGVRGWYLSEYSKHKYWAKDLMLYITSKDTLQ
                     KYTDEMSEITGRVDVKSSNPNLKVFEKQARHAEPMPNIPEMRQVWEPMGNASIFISNG
                     KNPKQALDEATNDITQNIKILHPSQNDKKGD"
     gene            complement(52537..53634)
                     /gene="ugpC"
                     /locus_tag="O2701_000044"
     CDS             complement(52537..53634)
                     /gene="ugpC"
                     /locus_tag="O2701_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HCZ4291964.1"
                     /translation="MAELKLEHIKKTYDNNNTVVKDFNLHITDKEFIVFVGPSGCGKS
                     TTLRMVAGLESITSEDFYIDGERMNDVEPKNRDIAMVFQNYALYPHMTVFENMAFGLK
                     LRKVNKKEIEQKVNEAAEILGLTEYLGRKPKALSGGQRQRVALGRAIVRDAKVFLMDE
                     PLSNLDAKLRVQMRTEILKLHKRLNTTTIYVTHDQTEALTMASRIVVLKDGDIMQVGT
                     PREIYDAPNCIFVAQFIGSPAMNMLNATVEMDGLKVGTHHFKLHNKKFEKLKAAGYLD
                     KEIILGIRAEDIHEEPIFIQTSPETQFESEVVVSELLGSEIMVHSTFQGMELISKLDS
                     RTQVMANDKITLAFDMNKCHFFDEKTGNLIV"
     gene            complement(54017..54595)
                     /locus_tag="O2701_000045"
     CDS             complement(54017..54595)
                     /locus_tag="O2701_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HCZ4291965.1"
                     /translation="MTKRPKRILATIIIFLSLLFTIIYIDDIQKWFNQYTDKLTQNHK
                     GQGHSKWEDFFRGSRITETFGKYQHSPFDGKHYGIDFALPKGTPIKAPTNGKVTRIFN
                     NELGGKVLQIAEDNGEYHQWYLHLDKYNVKVGDRVKAGDIIAYSGNTGIQTTGAHLHF
                     QRMKGGVGNAYAEDPKPFIDQLPDGERSLYDL"
     gene            54804..55430
                     /locus_tag="O2701_000046"
     CDS             54804..55430
                     /locus_tag="O2701_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCZ4291966.1"
                     /translation="MAKVLYITAHPFNELVSNSMAAGKAFIETYQQQHPDDEVKHIDL
                     FETYIPVIDKDVLTGWGKMSNGETLTDDEQMKVSRLSDILEEFLSADKYVFVTPMWNL
                     SFPPVVKAYIDAISIAGKTFKYSAEGPQGLLTDKKVLHIQSRGGYYTEGPAADFEMGD
                     RYLRTIMTFLGVPSYETIIIEGHNAEPHKTEEIKATSINNAEKLATTF"
     gene            55620..56393
                     /locus_tag="O2701_000047"
     CDS             55620..56393
                     /locus_tag="O2701_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCZ4291967.1"
                     /translation="MKAHKIFWLNLAAIIIISIVVSGDMFLAMKWEQIHLKDGLKKVL
                     STYPIKNLETLYEIDGHDNPHYENNDQDTWYIESSYSVVGSDELLKEDRMLLKVDKNT
                     HKITGEYDTTTNDKKNATDSTYKSYPVKVVNNKIVFTKDVKDPALKQKIENNQFLIQS
                     GDLTSILNSNDLKVTHDPTTDYYNLSGKLSNDNPNVKQLKRRYNIPKNASTKVELKGM
                     SDLKGNNHQDQKLYFYFSSPGKDQIIYKESLTYNKISEH"
     gene            complement(56691..58697)
                     /gene="ggt"
                     /locus_tag="O2701_000048"
     CDS             complement(56691..58697)
                     /gene="ggt"
                     /locus_tag="O2701_000048"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCZ4291968.1"
                     /translation="MVINLNDKQTKTSKEGLISVSHPLAAKIGKDVLDQGGNAMDAVI
                     AIQLALNVLEPFASGIGGGGYLLYYEQSTGSITAFDARETAPEHVDKQFYLDDSGEYK
                     SFFDMTTHGKTVAVPAIPKLFDYIHKRYAKLSLEDLINPAIELAIEGHAANWATEKYS
                     RQQHARLTKYHETAQVFTHENQYWREGDWIVQPELGKTFQILREQGFNAFYKGDIAKQ
                     LVNVVKACGGTITLEDLAKYDIQIKAPISATFKDYDIYSMGPSSSGGITVIQILKLLE
                     HVDLPSMGPRSVDYLHHLIQAMHLAYSDRAQYLADDNFHEVPVQSLIDDDYLKARSTL
                     IDSNKANIDIEHGVVSDCISHTDVEENHTETTHFCVIDKEGNIASFTTSIGMIYGSGI
                     TIPGYGVLLNTTMDGFDVVDGGINEIAPYKRPLSNMAPTIVMYHGKPILTVGAPGAIS
                     IIASVAQTLINVLVFGMDIQQAIDEPRIYSSHPNRIEWEPQFSQSTILALIAHGHAME
                     HKPDAYIGDVHGLQVDPTTYEASGGSDDTREGTVMGGEVLVIRKQPLPYRQMYDSDGF
                     RLYFNDVQLPLLADQVRWMHDKYWVDESVVRIIFPEVSAHIEDLRSYENAGENYIDIA
                     WLARKYAYQVTLKDDGLYLTDDTYTSVKRNTNAYYRYDRDSITR"
     gene            complement(58735..60510)
                     /locus_tag="O2701_000049"
     CDS             complement(58735..60510)
                     /locus_tag="O2701_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCZ4291969.1"
                     /translation="MKKIISIAIIVLALVLSGCGVPTKSEVAQKSSKVEVKGERPTIH
                     FLGQASYENDMNIVKDQLENAGFNVKMNIQPDYGSYRTQRQAGNYDIQIDDWMTVFGD
                     PNYAMTALFSSTGSNSLLKDKHVDQLLNKASTQNEADVKQTYKQIEDEVVFDKGYMAP
                     LYGSKKNLVYDNKVLDKNSVGLPNSRALIWQQFDYNNSRERDTRPLVMTQQDGEIPTL
                     DPIRSIAPSVYSINMNMYTRLLLLDENDHLTTKGSLSHDYAVNKDNKAFYFLLRDDDY
                     FAKVVNGQARNTGERVSAEDVKFSLDRARDKKSVPNNNTYNMHKHINDIKILKDEDID
                     QLRKEKDKDDKSIYDKLIKAYNVKSLTTDGQKVNNKDGIYQIVKITTDQSMPREVNYL
                     THSSAGILSKKFVNQVNQEYPKGYGDSSTIPANSDGKNALYASGAYIMTQKNAYQATF
                     QRNPGFNETEKGSYGPAKIKNITLKFNGDPNNALSELRNHSIDMLADVNQKHFDLIKS
                     DKNLSIIRKNGRKSVFLMLNIKKGIFKTHPNLRQAVVNAIDQDQFIKFYRGDKFKIAS
                     PITPLVDTGNEQRQDLEKVEKAINQ"
     gene            complement(60527..61690)
                     /locus_tag="O2701_000050"
     CDS             complement(60527..61690)
                     /locus_tag="O2701_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4291970.1"
                     /translation="MAQLNSKIASLKLFASYAIATYILVILTSALNLFKGYVADTFYI
                     AETLLIVLTIILIIILTTEQTWKHHDLWRRIVEVLLLLMTLTGNVFTLLMFVSIRRYQ
                     RTSQIHSYNGWESFIRKTTRHRIAIIGLLILVYMLTLSIVSQFTFDTTLATKNQFNAL
                     LHGPSLAYPFGTDDFGRDLFTRVVVGTKLTFSISIISVVIAVIFGVLLGTIAGYFNHI
                     DNLIMRILDVVFAIPSLLLAVAIIASFGASIPNLIIALSIGNIPSFARTMRASVLEIK
                     RMEYVDAARITGENTWNIIWRYILPNAIAPMIVRFSLNIGVVVLTTSSLSFLGLGVAP
                     DVAEWGNILRTGSNYLETHSNLAIVPGVCIMFVVLAFNFIGDAVRDALDPRIH"
     gene            complement(61696..63174)
                     /locus_tag="O2701_000051"
     CDS             complement(61696..63174)
                     /locus_tag="O2701_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4291971.1"
                     /translation="MNCTFRLLTSNKFDMMLCLPIHKCVNLFYTFVYHSNLLKIILQG
                     VIIIITYLESFKSLYHKAFYKFVLSVLSLPIFLYAVIKFFFSAKRKNFYANNSEISEI
                     EQALHQKYKYLSQQKSSTQIHKEALKIFKAQSSNTSSKNIEQAHFSTYFENVLFHKFI
                     MIKVILALPMFILLTFYLQPLVRYIFERIVMAVIVIIGVIVSVFTILYFSPLDAAYSI
                     LGQNATKAQIHQFNVLHHLNEPYFIQLWDTIKGVFTFDLGTTYKGNEVVTKAVGERIP
                     ITIIVAVLALMVALIIAIPIGIISAMKRNSWLDITLMIIALIGLSIPSFWQGLLFILA
                     FSLKLDILPPSYMPEHPISLILPVLVIGTSIAASITRMTRSSVLEVMRSDYVLTAYAK
                     GLSTTQVVIKHILKNAIIPIVTLVGLLVAELLGGSAVTEQVFNINGIGRYIVQKQLIP
                     DIPAVMGGVVYISIVISLANLIIDIFYALIDPKLRSEINERK"
     gene            63131..64723
                     /locus_tag="O2701_000052"
     CDS             63131..64723
                     /locus_tag="O2701_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4291972.1"
                     /translation="MSNLLEVNSLNVQFNYDETTVQAVKNVSFELRKKHILGIVGESG
                     SGKSITAKSILGLLPDYPDHTLTGEIIFNGQSLNNLSTSALQQIRGKDISMIFQDPLS
                     SLNPRLTIGKQITEVIFQHKRVSKSEAKSMTIDILEKVGIKHATRQFDAYPHELSGGM
                     RQRVMIAMALILKPQILIADEPTTALDASTQNQLLQLMKSLYEYTETSIIFITHDLGA
                     VYQFCDDVIVMKDGSVVESGTVESIFKSPQHTYTKRLIDAIPDIHQTRPPRPLNNDIL
                     LKFDRVSVDYTSPSGSLYRAVNDINLAIRKGETLGIVGESGSGKSTLAKTVVGLKEVS
                     EGFIWYNELPLSLFKDDELKSLRQEIQMIFQDPFASINPRFKVIDVIKRPLIIHGKVK
                     DNDDIIKTVVSLLEKVGLDQTFLYRYPHELSGGQRQRVSIARALAVEPKVIVCDEAVS
                     ALDVSIQKDIIELLKQLQLDFGITYLFITHDMGVINEICDRVAVMKNGEIVELNNTED
                     IIKHPQSDYAKQLISEVAVIAK"
     gene            complement(64988..66100)
                     /locus_tag="O2701_000053"
     CDS             complement(64988..66100)
                     /locus_tag="O2701_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HCZ4291973.1"
                     /translation="MKICEKCFNNTEIVEIIANDNSKFDNCDIDNNHLGVKIFDTTKD
                     IDKLELIRDYLRPALELYDISINLPDTFSPKEGKKIEIALKDDWSIFNVEEAQISCIL
                     NELFKDDENLDRRVLEDLVGAKIINDKKYTNKNLIVANNDWDGFCESLKYKNRFHNNM
                     INLENLAFFLDITTNYYSIEEFRERFEPLYRSRIVKFITEKSELMSPPKGFATAGRLN
                     SKWISVLYLSTKEQVSIEEVKPKHNDIIYIGKVKLQKNVTKEKLKIANLTNLTSNAIK
                     AGDDGFRKYFVNYQTLKKIHKGITNPSDEQGIDYLPFQYLADYIRSLKYDGIMYESIL
                     QDGTFNFVFFDQSLFECVNYERKRVSDVKYTLTKLS"
     gene            complement(66087..67010)
                     /locus_tag="O2701_000054"
     CDS             complement(66087..67010)
                     /locus_tag="O2701_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HCZ4291974.1"
                     /translation="MYYPYLRGKQNELFAIRELLEKGLIGDCIQPIIEPIKYTTTFKN
                     TLQYCGEKAFSINLVVNSKLTEEEISNETVAHLTEIITKNKSVIQKAYLGPSDEGNDR
                     LKQQFSSNSLAILTSVDDWEMFGDKNKLEMVFVPDDRHIKRKLRNIPNKGIIMDPFNK
                     LSRNVDYLDNDDEFYSDDHLYYKEDGYVAFSDYSVIGGEYVDGGFSPLAIAIHIVYFD
                     EANELRVKHFVSDSNNDRSNPGKKFFEAVDKLVTWSKNLDIKNRSYALGQFEELNENN
                     KYPGLGLIKRLSIMHHLEIMNRYLESQNENM"
     gene            complement(67012..67854)
                     /locus_tag="O2701_000055"
     CDS             complement(67012..67854)
                     /locus_tag="O2701_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HCZ4291975.1"
                     /translation="MDNRNMINRVFSQKILHQIAIKNKSDVVDEAYDFYIQGPKNINV
                     IQKMKSLYNYLKKSYRNEYFYKNTILNKLLLGRHSINTTTALSEMPIGKSIADFILLN
                     GKGVVYEIKTELDKLDRLDNQINDYYEVFNYVVVITNDKHLNKVMARYKDTTVGILVL
                     TTRNTLSEVQKPKENNSLLNTKAMYNFLRKEERKRVIAQNHMDVPTYNDFTEYDVLFD
                     VFKEIPMTKLHNNMISELKKRGNMKEYKDEFLAAPAEIKFLLYFAKMTKKDKNKLYHF
                     LKED"
     gene            complement(68059..70848)
                     /locus_tag="O2701_000056"
     CDS             complement(68059..70848)
                     /locus_tag="O2701_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCZ4291976.1"
                     /translation="MAYQSEYALENEMMNQLEQLGYERVTIRDNKQLLDNFRTILNER
                     HADKLEGNPLTDKEFQRLLTMIDGKSIFESARILRDKLPLRRDDESEIYLSFLDTKSW
                     CKNKFQVTNQVSVEDTYKARYDVTILINGLPLVQVELKRRGIDINEAFNQVKRYRKQN
                     YTGLFRYIQMFIISNGVETRYFSNNDSELLKSHMFYWSDKQNNRINTLQSFAESFMRP
                     CQLAKMISRYMIINETDRILMAMRPYQVYAVEALIQQATETGNNGYVWHTTGSGKTLT
                     SFKASQILSQQDDIKKVIFLVDRKDLDSQTEEEFNKFAKGAVDKTFNTSQLVRQLNDK
                     SLPLIVTTIQKMAKAIQGNAPLLEQYKTNKVVFIIDECHRSQFGDMHRLVKQHFKNAQ
                     YFGFTGTPRFPENSSQDGRTTADIFGRCLHTYLIRDAIHDGNVLGFSVDYINTFKNKA
                     LKAEDNSMVEAIDTEEVWLADKRVELVTRHIINNHDKYTRNRQYSSIFTVQSIHALIK
                     YYETFKRLNKKLEQPLTIAGIFTFKPNEDDRDGEVPYHSREKLEIMISDYNKKFETNF
                     STDTTNEYFNHISKNVKKGVKDSKIDILIVVNMFLTGFDSKVLNTLYVDKNLMYHDLI
                     QAYSRTNRVEKESKPFGKIVNYRDLKKETDDALRVFSQTNDTDTILMRSYEEYKKEFM
                     DAYRELKMIVPTPHMVDDIQDEEELKRFVEAYRLLAKIILRLKAFDEFEFTIDEIGMD
                     EQENEDYKSKYLAVYDQVKRATAEKNKVSILNDIDFEIEMMRNDTINVNYIMNILRQI
                     DLEDKAEQRRNQEQIRRILDHADDPTLRLKRDLIREFIDNVVPSLNKDDDIDQEYVNF
                     ESIKKEAEFKGFAGERSIDEQALKTISNDYQYSGVVNPHHLKKMIGDLPLKEKRKARK
                     AIESFVAETTEKYGV"
     gene            71061..71342
                     /locus_tag="O2701_000057"
     CDS             71061..71342
                     /locus_tag="O2701_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4291977.1"
                     /translation="MVLITYQIILFFIISLSYYLTLNHYMAVTVGNFTSIFGMFAAIL
                     FMYYYLLYKSPEYNQRKRFKHFIHITNLIIIAFSTFVLVHLALKLFFSI"
     gene            complement(71536..72411)
                     /locus_tag="O2701_000058"
     CDS             complement(71536..72411)
                     /locus_tag="O2701_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCZ4291978.1"
                     /translation="MTNILYRIDKQLSDFTKTEKIIADYILKNPHKIIDMTVNDLADV
                     TNVSTASIVRFSRKMTHQGFQELKIAISRYLPEDIATNPHLELIENESVETLKNKMIA
                     RATNTMRFVATNIMDAQIDAICDVLKNARTIFLFGFGASSLTIGDLFQKLSRIGLNVR
                     LLHETHLLVSTFATHDDRDCMIFVTNQGSHSELQSIAQVATHYSIPIITISSTANNPV
                     AQIADYALIYGRTDENEMRMAATTSLFAQLFTVDILYYRFVALNYHAILDCITQSKMA
                     LDNYRKHLATIDFKH"
     gene            complement(72414..73868)
                     /locus_tag="O2701_000059"
     CDS             complement(72414..73868)
                     /locus_tag="O2701_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCZ4291979.1"
                     /translation="MTKEQQLAERIIAAVGGMDNIDSVMNCMTRVRIKVLDENKVDDQ
                     ELRHIDGVMGVIHDERIQVVVGPGTVNKVANHMAELSGVKLGDPIPHHHNDSEKMDYK
                     SYAADKAKANKEAHKAKQKNGKLNKVLKSIANIFIPLIPAFIGAGLIGGIAAVLSNLM
                     VAGYISGAWITQLITVFNVIKDGMLAYLAIFTGINAAKEFGATPGLGGVIGGTTLLTG
                     IAGKNILMNVFTGEPLQPGQGGIIGVIFAVWILSIVEKRLHKIVPNAIDIIVTPTIAL
                     LIVGLLTIFIFMPLAGFVSDSLVSVVNGIISIGGVFSGFIIGASFLPLVMLGLHHIFT
                     PIHIEMINQSGATYLLPIAAMAGAGQVGAALALWVRCKRNTTLRNTLKGALPVGFLGI
                     GEPLIYGVTLPLGRPFLTACIGGGIGGAVIGGIGHIGAKAIGPSGVSLLPLISDNMYL
                     GYIAGLLAAYAGGFVCTYLFGTTKAMRQTDLLGD"
     gene            complement(73880..74776)
                     /gene="murQ"
                     /locus_tag="O2701_000060"
     CDS             complement(73880..74776)
                     /gene="murQ"
                     /locus_tag="O2701_000060"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCZ4291980.1"
                     /translation="MENSTTEARNEATMHLDEMTVEEALITMNKEDQQVPLAVRKAIP
                     QLTKVIKKTIAQYKKGGRLIYIGAGTSGRLGVLDAAECVPTFNTDPHEIIGIIAGGQH
                     AMTMAVEGAEDHKKLAEEDLKNIDLTSKDVVIGIAASGKTPYVIGGLTFANTIGATTV
                     SISCNEHAVISEIAQYPVEVKVGPEVLTGSTRLKSGTAQKLILNMISTITMVGVGKVY
                     DNLMIDVKATNQKLIDRSVRIIQEICAITYDEAMALYQVSEHDVKVATVMGMCGISKE
                     EATRRLLNNGDIVKRAIRDRQP"
     gene            complement(74776..75831)
                     /locus_tag="O2701_000061"
     CDS             complement(74776..75831)
                     /locus_tag="O2701_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCZ4291981.1"
                     /translation="MTGFSVYLGQPLDEAYIKRMIKQGYQMIFTSVQIPEEDDETKYH
                     YFTKLLNLLKHEQVTYLIDANPSILTPSFYDHLRQYDAQFMIRIDHSTSIEAIEAIMA
                     QGLKCCLNASIISRELLTSLHQQLNDFTLLSFCHNYYPRPDTGLSVDLVNKKNELIYQ
                     FNPKAQIYGFIVGSDLRGPLHKGLPTIEATRHSHPVVAAKLLQETGVSEVLVGDSLIE
                     MRQAKQLIDFCKHRHFTLCIEEVFDTTVTYLFDMCHKVRPDNPENVIRSETSRQICPH
                     SIQPQFTTQRRIGSVTVDNLNNGRYQGEMQIVRQTLSAHDNVNVVAQIIKEDLPLLSC
                     IEPNDTFDFQKTRECKK"
     gene            76417..78462
                     /locus_tag="O2701_000062"
     CDS             76417..78462
                     /locus_tag="O2701_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HCZ4291982.1"
                     /translation="MRKKLFGQLQRIGKALMLPVAILPAAGLLLAIGTAMQGESLQHY
                     LPFIQNGGVQTVAKLMTGAGGIIFDNLPMIFALGVAIGLAGGDGVAAIAAFVGYIIMN
                     KTMGDFLQVTPKNIGDPASGYASILGIPTLQTGVFGGIIIGALAAWCYNKFYNINLPS
                     YLGFFAGKRFVPIMMATTSFILAFPMALIWPTIQSGLNAFSTGLLDSNTGVAVFLFGF
                     IKRLLIPFGLHHIFHAPFWFEFGSWKNAAGEIIHGDQRIFIEQIREGAHLTAGKFMQG
                     EFPVMMFGLPAAALAIYHTAKPENKKVVAGLMGSAALTSFLTGITEPLEFSFLFVAPL
                     LFFIHAVLDGLSFLTLYLLDLHLGYTFSGGFIDYFLLGILPNKTQWWLVIPVGLVYAV
                     IYYFVFRFLIVKLKYKTPGREDKQSQAATASATELPYAVLEAMGGKANIKHLDACITR
                     LRVEVNDKSKVDVPGLKDLGASGVLEVGNNMQAIFGPKSDQIKHEMQQIMNGQVVENP
                     TTMEDDKDETVVVAEDKSATSELSHIVHAPLTGEVTPLSEVPDQVFSEKMMGDGIAIK
                     PSQGEVRAPFNGKVQMIFPTKHAIGLVSDSGLELLIHIGLDTVKLNGEGFTLHVEEGQ
                     EVKQGDLLINFDLDYIRNHAKSDITPIIVTQGNITNLDFKQGEHGNISFGDQLFEAK"
     gene            complement(78441..78542)
                     /locus_tag="O2701_000063"
     CDS             complement(78441..78542)
                     /locus_tag="O2701_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4291983.1"
                     /translation="MMLQNTIAKKKQARHLMKVYAPVYSKHYLASNN"
     gene            78736..80376
                     /locus_tag="O2701_000064"
     CDS             78736..80376
                     /locus_tag="O2701_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-keto acid decarboxylase family protein"
                     /protein_id="HCZ4291984.1"
                     /translation="MKQRIGAYLIDAIHRAGVDKIFGVPGDFNLAFLDDIISNPNVDW
                     VGNTNELNASYAADGYARLNGLAALVTTFGVGELSAVNGIAGSYAERIPVIAITGAPT
                     RAVEQGGKYVHHSLGEGTFDDYRKMFAHITVAQGYITPENATTEIPRLINTAIAERRP
                     VHLHLPIDVAISEIEIPTPFEVTATKYTDASTYIELLATKLHQAKQPIIITGHEINSF
                     HLHQELEDFVNQTQIPVAQLSLGKGAFNEENPYYMGIYDGKIAEDKIRDYVDNSDLIL
                     NIGAKLTDSATAGFSYQFNIDDVVMLNHHNIKIDDVTNDEISLPSLLKQLSNISYTNN
                     ATFPAYHRPTSPDYTVGTEPLTQQTYFKMMQNFLKPNDVIIADQGTSFFGAYDLALYK
                     NNTFIGQPLWGSIGYTLPATLGSQLADKDRRNLLLIGDGSLQLTVQAISTMIRQHIKP
                     VLFVINNDGYTVERLIHGMYEPYNEIHMWDYKALPAVFGGKNVEIHDVESSKDLQDTF
                     NAINGHPDVMHFVEVKMAVEDAPKKLIDIAKAFSQQNK"
     gene            80443..81000
                     /locus_tag="O2701_000065"
     CDS             80443..81000
                     /locus_tag="O2701_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCZ4291985.1"
                     /translation="MINFNKTALVLIDLQEGILKMDYAPYTAENVVQNANKLIDVFRK
                     NNGFIAFVRVNFYDGKDVLQPNAMISLPPKEGDDYSRFHHLLDKRDDDFVIDKRHFSA
                     FVGTDLDLQLRRRGIDTIVLGGVATHIGVDTTARDAYQLNYNQFFVTDMMSAQNETLH
                     QFPIDNVFPLMGQTITTNDFLNILN"
BASE COUNT        25921 a        15147 c        12728 g        27370 t
ORIGIN      
        1 atttcgttgc attttccaat tgaaaactca ttttaaaatc aaaactctaa atgtctgtgt
       61 attacttaaa attatacata ttttgcttat attttagcat attttgttta aacctatatt
      121 acattatatc agacgttttc atacacaaat aataacatac aagcaaacat ttcgtttatt
      181 atttatatca cttaactaat taatttataa ttttttattg tttttaagtt atcacttaaa
      241 aatcgtttgg caaattcgtt gtgacgcttg tccatcttct aatgaacaga atttttgata
      301 aaataccgtt cgtgcttcaa tatactcatt tgcagtctca tcgatttgtt ttaatgcatc
      361 aatgagtgct gtttgatttt caacaattgg acctggcaac tcttttttat aatccatgta
      421 aaaacctcta agctcatcgc catatttatc taagtcatat gcatagaaaa tttgcggacg
      481 ctttaataca ccgaagtcga acatgacaga tgagtagtcg gtaactaacg catcgctgat
      541 taagtataaa tccgaaatgt cttcataatc tgaaacgtct ttcacaaaat catcatgttc
      601 atcaatacgt gtcacaacta aataatgcat gcgtaataaa ataacataat catcatccag
      661 cgcttgacgc aaagcttcta tatcaaagtt aacattaaat tgatatgaac cttctcgaat
      721 cgcttcatcg tcacgccaag ttggcgcgta cataatcact tttttatcta atggaatatt
      781 taatcttgtc ttaataccat taatatattc agtatcattg cgtttatgtg ataatttatc
      841 atttcttgga taacctgttt ccaaaatctt atctcgacta acatgaaatg cattttgaaa
      901 tatcgatgtc gaatatggat taggtgacac tagataatcc caccgttggc tttctttttt
      961 aaagccatct tggtaatttt gagtatttgt tcctagcatt ttaacgttac taatatccaa
     1021 accaatcttt tttaatggcg tgccatgcca tgtttgtaag tacgtcgttc gcggtgattt
     1081 atataaccaa tctggtgtac gtgtgttaat catccacgct ttcgctcttg gcatcgctaa
     1141 aaaccatttc attgaaaact ttgtaacata tggtacattg tgctgttgga atatgtgttc
     1201 atatcctttt ttcacacccc atattaattg ggcatcgcta tgttcagtta agtattcata
     1261 taatgctttg gggttgtcgc tgtattgttt accatgaaag ctttcaaaat aaattagatt
     1321 cttgtttggc aattttgata gtaatttaaa agtcgtatat atactatgtt ctatcaattt
     1381 tttaattgta tttttaatca tgtcgtacct ccgacgtgtt tttgtaatta tattaatatg
     1441 tatgagcaag ctcattgtaa ccatgcctat tatagcattt catcataaaa tacatttaac
     1501 cattacactt gtcgttaatt atcatacgaa atacatgatt aatgtaccac tttaacataa
     1561 caaaaaatcg ttatccattc ataacgtatg tgtttacaca tttatgaatt agataacgat
     1621 tggatcgatt attttatttt acaaaatgac aattcagttg gaaggtgatt gcttttgatt
     1681 gaatcgcctt atgcatgaaa aatcaaaagg ttattctcat tgtatagtcc tgcttctcat
     1741 catgacatgt tgctcacttc attgtcagaa cccttcttga aaactatgcc ttatgactca
     1801 tttgcatggc aagtaatata tgccaacatt agcgtctaaa caaatctttg actaaacgtt
     1861 cacttgagcg accatcttga tatttaaaat gtttatctaa gaatggcaca actttttcaa
     1921 cctcataatc ttcattgtcc aaagcatcca ttaatgcatc aaaggactgt acaattttac
     1981 ctggaacaaa tgattcaaat ggttcataga aatcacgcgt cgtaatgtaa tcttctaagt
     2041 caaatgcata gaaaatcatc ggctttttaa atactgcata ttcatatatt aaagatgaat
     2101 aatcactaat caacaagtct gtaacaaaga gaatatcgtt aacttcacga tgatctgaca
     2161 catcgataaa gtattgtcta tgttcacgtg aaatattaag tctatttttt acgaacggat
     2221 gcattttgaa taacacaact gcattatgct tctcgcagta tcttgctaaa cgttcaaaat
     2281 caattttaaa aaatggataa tgtgccgtac cgtgaccatt acctctaaat gtcggtgcga
     2341 atagaataac tttcttacct tttataattg gcaattcatc ttccatctct tgtttaattt
     2401 gtgttgcata agcttcatca aatagtacat cagtacgtgg tacacctgtt ggtacaacat
     2461 tttcttctct aattccaaat gcttcagcat aaaatggaat atcggtttct gatgaaacat
     2521 atgctttcgt gtaactacga tggtttaatg aatcgataaa cggaccacct tttttacctg
     2581 tacgactaaa gccaactgtt ttaaaggcac caacagcatg ccacacttga ataatttctt
     2641 gtgatggtct aaagcgaacc gtataaatta atggatggaa atcatcaaca aaaatataat
     2701 cggccttacc aagtaaatat ggcaatctaa acttgtctct ccatttgcgt ctatccgtaa
     2761 tattctcctt aaataccgtt ttaatatcat aatcaaaatc tactttttgg cgtagtaact
     2821 catcatatac atacttgaaa ttccctgata aattcggacg cgaatctgat gtgaataata
     2881 ttgttttgct tcttttaata tgtagtaact ttgtaatatt aaaaatagct ttaaataaga
     2941 aacttctact ttcaaatgaa gctttatgac cttgtttatg aagccagtgt gcacttgtcg
     3001 caatgacccc tgatttctct tgaggtaagg tgatttcaat atcaaataca aattcgttaa
     3061 cgtcacttgc tatttccgga gtaatggtat agaccgtatg ttgtgatacg ccacctttac
     3121 ggaaaacttt agcatcatac gctaataaat agttcacttt tccattttgt gtagtcagct
     3181 cattgtattc ttcaatttgc tcttcattta atgccgcata agcttgatca ataatatttg
     3241 gattaagttg tccaatatac tctaactggt catgataaac aaataaatat ttatccattg
     3301 gtaaataata cccatcgtct aatattgcga cgttgaatct acatataaat gtattaccgt
     3361 ctattttcat ttcatttgct tctaatgttt ttgtttcggt taaattcctc agaacaaatt
     3421 gtcctgatgt taaatcgaca ccttcactat gtccttcaat atataactga acacgttccc
     3481 agtatatgtt atcaatatgt attgcttgtt tcgtttttgt cattgttatt cccccatcgg
     3541 tttattaaat cgtccatttc aatactgttt ttccccaaga tgtcgataaa tccatttcaa
     3601 acgcttggac gatatcttgc atcgtacata cattaatttc atgtcctttt aataatgcta
     3661 acttttcaac tatgtctggg tacttacgat ataaatcaac aacttgctca aaatctttag
     3721 agccgcttcg actactacca atcaacgtta atcctttttc aagtactaat cgtgtattca
     3781 cttccacggg taattcactt acgcctaaca aagcaatact gccttctggt gaaatatgtt
     3841 caactatttg ttgaagtgca acttgacttc ctttacctcc aacacattca aatgcatgat
     3901 caattttaag atcatctggt atttgattta ctgtaaagat gtcatctaca aatgaaaaat
     3961 gacttaattt atagtctgtc ttaccaaata cataagtttt agcttctggg tacaacttac
     4021 gtagcaaaat agcagtaata taacctaagt taccatcacc ccaaatacca aagctggttt
     4081 tcaaaggtat agatttacgt tcaaatcgtt gtatagcatg ataacttact gacactaact
     4141 ctgtgtatga aatcgtactc aaatcaatgt cattaggcag cggaacgata cgatcatgtg
     4201 ccatcacaac gtagtcttgc ataaaaccat cataaccact agatctaaaa taactagagg
     4261 ctaagtaatt ctccgcaata atatgatgtt gctctgtagg tgtattcggt accattacta
     4321 ctttcgtacc tttttcaaat acccctttac tatcaaatac aacttcacca acagcttcat
     4381 gaactaatga cattggtaat tttttgcgta gtacattttc atctcttcga cctgtgtaat
     4441 acctttgatc agctgcacaa atagacaagt ataaaggtct tacgatgaca tgattaccat
     4501 aaatatcaac attattatat gtgacgtcga actgtctcgg tgcaacgagt tgatatactt
     4561 gattaatcat cggcaatatc accttgaata atggcatttg ctacttttaa atcatacggt
     4621 gttgtcactt taatgttgta tagttctcca cgtaccaatt taactgcatg tccagattcg
     4681 acaatgattt tacatgcatc tgataagatt tctttttgtt cactacttaa ggcgcgataa
     4741 ctatcttgta ataatttaat attaaatgat tgtggtgttt ggccttgata catttcattc
     4801 cttacaggga tactgtgtat gttctgttta tctttagaca ttacaatcgt atcaattgct
     4861 tcaatgactg tatctactgc accatatttt gctgctactt caatgttctc tttaataata
     4921 cgttgagtta aaaatggtct tacggcatca tgagttacaa tcacatcatc attattaatt
     4981 ccatttacat tgcgaatatg gtcgataatg ttcataattg tttcatttcg atccgtacca
     5041 cctgcaacta ctttgacacg ttgatctgta atgttatatt tttttaaaat atcctgtgta
     5101 tgggaaatcc actgtgctgg cgttgcgata ataatctcat taaattcact cactaaaatg
     5161 aacttctcaa ttgtatggat taaaatcggt ttattatcaa tatctaaaaa ttgttttggt
     5221 aatggcacgt tccccattct cgaaccaata cctcctgcta aaatacctgc ataaatcatg
     5281 ttgtcctcca ttctgtcatt acatcatttc catttataca ttactgacct atgcccgcac
     5341 ataagcctaa cctattgctc acttgcctct tttattaatc caaagatagt tgtcacaata
     5401 gtgtgataat tttttataaa aatgtatttt tgtaactgac cattctaagt tgttttgcca
     5461 tgcagttaat cattaactct gacgatatta aattgttaaa ggtattaatg tttactcttt
     5521 ttcaaattca ttattactgc catcatttta ccatatatta taataaattt atcttattaa
     5581 gtggctgtac ttgattttca ctttaaaaat tatcaaatat tgccatctca ttttaagtat
     5641 acaaaatgca aaacaaccga ttcacaagca tatttcacac aagtaaaccg gctatttatc
     5701 aacgtatatt cgaagatgaa ttatttcgat agtatctata gaccagacgg cattcgcact
     5761 ttcatagcta taactatacc agcgttttcg tcctcaaagg tgcatactaa taaatcgtaa
     5821 acatgacttt atcaaatcgt tctttcttgt taactaattt atcaaatgtc tccgggcctt
     5881 tttctaacgg taaaaaatga gaaataatag gctttacatt aatatctttc gtcttcatat
     5941 aatgtaaggt tgccgtccac tctttgcccg gaaaattact ggacaaacag ttccaagagc
     6001 cacatactgt caactcgtta cgcagaattt tttcaaaatg aacgcgatca atctcaatat
     6061 catcatatgg tattccgagt aataccacct cgccaccttt tttaggtagc gtcaatattt
     6121 gaccaatcgt aactttagca cctgatgatt ctatagctaa atcgatttga ttggcgtaat
     6181 gattttcgat gaatttctca agattttctt cttttgaatt gattgtttga tgtgcgccca
     6241 atgatgttgc aatatctagt ttatgcgcat ctatatctat agcgatgata tgtgcagcac
     6301 caaatattcg tgcccattga atagctaaca aacctatact gccacacccc attactgcaa
     6361 cagtcatacc aggttgtata ttcgatttat aaaacccatg cgcaacaacg gctgatggct
     6421 caaccattgc tgcttcaatg taatcaacat tgtctggaac ctttaaaaca ttttgcgctg
     6481 gcaatttgac atattccgcg aacgatccag gttcatatga gccaatgacg aataactttt
     6541 cacatcgtgc atattcacct tttaaacaat actcgcattg ataacaaggt attgctgggc
     6601 aacctgtcac tttgtcgccc acattaacat gcgtaacatc acttccaatg gcatctacta
     6661 cacctgaaaa ttcatgacca aatggcatac ctttaatgta tggccccatt tttttgtatc
     6721 gtgacgtgtc tgaaccacat atgccagtcg ctcgtacttt aataataacg tcattcgcac
     6781 tttcaatgac tggcttttca ttatcctcat accgtaaatc ttccacgcca tataatttca
     6841 atgctttcac ttgtaaatca cctcaaattt gatttaattc acaacttttt tctttttaaa
     6901 aatacctgtc gcaaaataac ctgcaatgac aatggaatta cttacgagta aatgttccat
     6961 ataaaaatca gtgatttgtc ttaatggccc aagcataaaa gttagcattg attctaccat
     7021 attaattctc tccttcattt tcatctaaat cgagcggctt aagtaatact ttaatacctt
     7081 cgccggattt taaatgttga tacgcctcgt cccatttcga aatatcatat atttttgtca
     7141 ccaaagcttc agcatttact aaaccatccg ccataagttg caatgaaggt tcccaatctg
     7201 ctggcttttg acttctacta ccaacaactg ttatttcttt ttgaatcact ttttccatat
     7261 caaatggaat ttcagcatcc ttaaaaatac ctatttgact gtagaaacct tttttgcgta
     7321 aaatatccaa accttgtcgt gctgctggaa ctgcacctga acattcaaca acaacatctg
     7381 caccgtaacc gtctgtaatt ccattgatat acgtttttaa gtctgtttgt tgtaaattga
     7441 ctacataatc catgtgcaat gcttctgctt tatctaatct gactttgtca ttgtccaatc
     7501 cagttaccac aacagttgcg cctttacttt ttaacacttg tgctacaagt aatccgattg
     7561 gcccaggtcc cattacaact gctacatcgc ctgaattgac ttgaatctta gaaacgccat
     7621 gatatgcaca tgctaatggt tctgtcatag ctgcagactg atacgatact tcgtctggaa
     7681 tatgatgcaa actttcttca cgtgcaatga cataattagt aaatgcgcca tcaacttgtg
     7741 ttccaatacc ttttcgatgg ttgcataaat tatagtcttt tgatttacag tattcacact
     7801 cattacaaac atagaatgtc gtttcagatg tgacacggtc accaacttta aaatctttaa
     7861 cgtctgctcc aacttcaacg atttcaccag aaaattcatg acctaatgtc actggaaaat
     7921 taactttata atgaccttca taagtatgaa tatctgtgcc acaaattcct gcataatgta
     7981 ctttaatctt tactttatca tctagcggtg ttgcaacttc tttatcaaga agttctaagt
     8041 tgccatgtcc ttctcttgtt tttactaaag ctttcaccac aaacacctcg atttttaatt
     8101 gaatagacta aatagtttaa agataagata gttaacgata ttaccacctt gatcaatact
     8161 tgaaatttca gatgaacctt ttggcatttg tacattcgta cctttcgcca tatctgtgaa
     8221 aatgggtgct acgtctgttg caatatatag tgaaattgca atcataatcg tacccacaat
     8281 gacagaatga ataatgtttc ctcttgctgc accaacaata aacgcgacaa caaatggtat
     8341 cgttgctaag tcaccaaaag gtagtacttg gtttcctggt aaaataacgg ctaataaaac
     8401 agtgataggt actaaaatta atgctgtcga aataactgct ggatgaccta atgctacagc
     8461 cgcatccaat ccaatataaa tttcacgttc gccaaaacgt ttatttagcc atgttcttgc
     8521 agactctgaa actggcatta aaccttccat taagattttt accattctag gcattaatac
     8581 cattactgca gccattgaca ttcctaaatt aatgatgtct ccaggtttgt aacctgctaa
     8641 cacaccaata cctaaaccta aaatcaagcc gacaaatata gactctccaa atgcgccaaa
     8701 acgtttttga attgtttcag gatcagcatc taacttattc agaccgggta ctttttgtaa
     8761 caatttaact aagtaaatac ctggtgcata agaaattgta cttcctgtag caatcgatac
     8821 acctggtaaa tcaaagaact cactcatcat cggtgctgtc caatctgcta ctttcaaaca
     8881 gataatttgg aaaataactg ctgctaataa cgcttgccaa atactgcctg atacggcata
     8941 aaccattgct gctgtaaacg tataatgcca aaaattccaa atatctacat tcatcgtctt
     9001 tgtcacttta gttactagca atacaacgtt aactatgatt ccgagtggaa taataaatgc
     9061 tgcgacagat gatgcccaag cgatagatga tgttgctggc caacctacat caatcacatt
     9121 cagactgacg cctaaatttt taaccatcgc ttgtgctgct ggccctaaat ttttaactaa
     9181 taaatcgatg actaagaaaa tccctacaaa agccacacct attgttaaac cagacctaaa
     9241 tgccgctcca attttctgcc taaagaatag gccaagcaag aatatgacaa ccggtaaaat
     9301 aacagttgca cctaaatcta aaaatcccct tacaaaatca gtgaagtaac tcatatttaa
     9361 accctccctg ttatatatgc attgtcacga tactttccga ttgtgattac atttgacgtt
     9421 acagtcattt caacgacaac ccttgctaaa ttcgactgca gtccttttga attacagtca
     9481 ctgcgtttct atgtcatcaa caatcatttg tcgtgatagt catttatatg caatttgcat
     9541 atattaatat gttatcgacc cacgttacat atcaattccg ttatttttgt aactctgtta
     9601 agatttgttg ttttgtttct tcaataccaa taccagttaa gaaattacgt gcgttgataa
     9661 ctgggaattt atattctttt tttgtcattg cagttgtaac taataaatct gcagtgtctt
     9721 cataaggtcc aacttctgta attttgattt gtttaatatc tactttaata ttgtgttcct
     9781 ttgccatttc ttcaattgca ttatttacta ctgttgacgt tgcaatacct gcaccacacg
     9841 ctactaatac ttgtttcatt ttcaattcct ccaattaatt tttagttata ttccaaataa
     9901 tcattgatta gtgttgctaa aattgtttca tctttcgttc gtagaatctg ctccaatttt
     9961 tcttcacttt gaaaaatttg catcaactgt tgtaacagct taagttgatc atctacttta
    10021 tccattgcta acataaaaac gattttcact tctgtctgtt gatcaagtgt tcccatttca
    10081 ataaacggca cttctttttc tagaacagcc acacctatcg ttctatggtt aatatgttcg
    10141 acatctgtat gcggtatagc gaccgaacat agatgcgttg gtaaaccagt agcaaattct
    10201 ttttctctgt cgatgactgc atctttaaac gttgacttca cgaacccatt ttgaaataac
    10261 acatctgaca tttgtgacaa tacggattct ttatcagttg ccgacaaatt gagcattata
    10321 ttttctttat gcactaattg ctgtcccatc cattttccct cgcttcttta tttgaataat
    10381 tttttaaaat ctcatttaca tcagaatttt tgcgactttg tatgatgcgc ttaattgcgt
    10441 cattgtcttg cgccacatct ctcaattgta gtaacgctct taagtgtgtc actttatcaa
    10501 cagcagcaat aggtacaata atatggattg ctgtgccatc tgacatgtat attggttctt
    10561 gtaatatcaa catactcatc gctgttttat gtacatgctt ttcagagtat gcatgtggaa
    10621 tagcaatatt ttgcatgata accatatacg aatcatcaaa tacctgttgc atctcatgaa
    10681 tatatttaat atcaataaaa tgattagcaa ctaacacatc acttgcttta gcaatagctt
    10741 catcaatatt ttcaacatga tgcattcttt tcacgtgcct tgccggtatc aagtcagcta
    10801 aatctaatgt cttattttgt gtgacaatcg atccattaat ggttgaaatt gaattataat
    10861 tggcaataaa atcttctaaa ccatcacgta gttctgtaat gtcattaact gtcgttgtgc
    10921 gttcaattaa tgccattaac ttgtttattt ccttatcaat gtcagccgat tccttattaa
    10981 tgtacttcat cacttcttta cgtaactttc gttgctcatt ttcagataaa gctacttttg
    11041 tgataaataa ttttttatgt gttaggacaa acattggtga aaagacgatg tcataatcta
    11101 atgtgtaatt ttcaaatgtt ctaagtgaaa tcgcatctaa gaaaataatt tctggaaata
    11161 agtttcgcaa ctcgtataac atcatttgtg atactgacgt gccttgtgta cacacgataa
    11221 tagcttttat cttgccatcg aagttttcat cttgacgtct caaactacct ccgaacaaca
    11281 tggttaaata tgctatttca ttatcaggca acgattttcc gaaatattca gttaacgatt
    11341 gacatgattg tttcaccata tgaaataagg attgataatt tccttgtaaa ggatttatta
    11401 attcatcacg atccgttaag ttatatttaa tcctataaaa agcaggcgtt aaatgtaaca
    11461 agagttgctg tgataatttc tccttatctt caatgttaat aaaagtgatt tgttcaaaat
    11521 ggtgaatcat ttgagcgatg gccatcgtta aattcgatat gctatctgat tcttgcaaat
    11581 cagtccattg cacacttgtt gaaagtaagt gtaatgtcaa atataacttt tccgcttctg
    11641 gcaaatccgg ctcatgttgc gtcataatct ccgttgcttg atattctttc gtatccctca
    11701 aatactgata attaatattt aatggattca tcacatgacc actttgaatt cgtctacgaa
    11761 tcacacaaag gacataaggc aatgaactaa gtgatttgtc tataaagcga ctcttcaaaa
    11821 attgttctac ctgtttgatc ttgtcttttt gatatgcgat atcttcgaat gttaagttga
    11881 gcgcctttaa aacttcactt ttagtaatat catgattcaa cctttgatca atcaacttaa
    11941 tgaagaaacg gcgaacttca aattcatcac caacaatttc ataaccatgt tttcgagaat
    12001 acttaagtga caaaccatga ttttccaatt gctctttcac atgatttata tcgtgaatga
    12061 cagtattttt actgacttgt aaatcaattg aaaaatggtt tagagacatt gcgttttcct
    12121 tactaaaaag catgagcatt aaataataac gacgtgtttc tatgctaaaa atgacattgt
    12181 tgccgtttaa catttgctgc tccgatacat ctcgcttgaa taacgtcatg atttcagaac
    12241 ttacaataaa atttccttgg cttgttcttt caagttttgg ataaccctct tgttcaagcc
    12301 acaaattgat tttttgaatg cgatatccta gttgtctacg agacaaacca aatatcgatt
    12361 caagttcttt accatgaata gtaggattca atacaatttc tctgagtatt tgctgattat
    12421 ccccgttcat gttcgattct cctttgagca tctgagctca atttatcatc aatcgtcaca
    12481 ccgatgtaca cactttgaac acatattttc aaaatgagca tgtacatcat tgtgatgttt
    12541 taacaacatt tcaattatat ctatattttt tgtgatttta atcttttaaa ataaagcaat
    12601 tgaaattttt gcatatattt ttgtgttttg tgtttttttg aagcattttt aacatacata
    12661 tctcaatcat tatcaaattg tcatgaccat tgtaacccaa tacaaaaacc ctaaggacgc
    12721 ttatatcagg cgccttaggg ttaactgtat ctatttaatt aagtattatt attcgtatgt
    12781 acgtaactta tggtctatca agttccacac ttcttcaaca tcaactgctg tagcaaaata
    12841 agcattggca ggcttacctg taacatgatt taaatcgaca gccatagtgc cataagttag
    12901 tggactttga tgttcaatgt cgatattaac gggtaccatt gtaaacaatt ctggttgtaa
    12961 caaatacaaa attgtacaag catcatgtat tggaccacca tccatattaa agtgagtctt
    13021 gtatgtcttc ttaaagaatt gcaataattc tacgacgaac tgtgcaacag gattattgat
    13081 actttcaaag cgttcaatca cgtgatcgtc ggctaaaact tgatgtgtta catctaaacc
    13141 aaacacattt atagtaatcc cactttcaaa aacacgcttc gctgcttcag catctaccca
    13201 aatattgaat tctgctgtag gcgtccaatt tccaaatgta ccaccaccca tcaaagtaat
    13261 agattcaata tgctcagcga ttcttggctc acgaatcaat gccgttgcta cattcgtaag
    13321 aggacctgtc gctacaattg ttacaggtgt atcactcgtc atcactttgt ttataatcac
    13381 atctgatgct ggcattgcaa ctgcttgacg tgatggtgtc gacggtagtt tcggaccatc
    13441 taatccagat tccccatgta tttcagaagc aaaggcagct ggtttaatta acggcctatc
    13501 cgcacctttc gctactgcta tatcttggcg tcccataata tccaatacgt tcaaggcgtt
    13561 tgtcgtattc ttgtcaactg attgattacc tgcgactgtt gttacagcta atatctctag
    13621 tggactgtca attgcccccg ctaaaattaa tgctattgca tcatcgtgtc ctggatcaca
    13681 atccataata atctttcttt tcatttatat atccaccttt cttaagttgt tatcgatagc
    13741 ttatgtatat ttatttatgt ggtgaatcat gtttattttg aaaaatagtt ttaactttct
    13801 catatttttg gatacaaaca ctatttatct attttatggc ttataaattt atccgatatg
    13861 ccttatcaac ctacctcgct aaaaatagga tgtctacata tctataccga cttttgtcaa
    13921 ctcattttca caacaatata aacagcaatt tatatgattg ttacatgatt caaacaattt
    13981 ttatgaaaaa tattttcata cacagaatat atattgatat taaatttctc aaaagctata
    14041 ttgagaataa ttaggaggga tgttgatgaa atctttattt gaaaaagcac agcagttcgg
    14101 caagtccttt atgttaccta tcgcaatctt accagctgca ggtctattgt tgggtatcgg
    14161 tggtgcatta agtaatccaa acaccgttaa agcataccct attttagata ttaccttatt
    14221 acaaaatatt tttacattaa tgtcagctgc aggtagtatt gttttccaaa atttaccggt
    14281 catctttgca attggtgtcg caatcggatt atctagaagc gataaaggta ctgcaggttt
    14341 agctgcgctg ctcggtttct taattatgaa cgcaactatg aatggcttat taactatcac
    14401 gggcacattg gcaaaagatc agcttgcaca aaatggacaa ggcatggtgc tcggtataca
    14461 aacggttgaa accggtgttt ttggcgggat tatcacaggt attatgaccg caatacttca
    14521 caacaaatat cacaaagtgg tattaccacc gtatttaggt ttctttggtg gctctagatt
    14581 tgtccctatt gtcacagcat ttgccgcaat ctttttaggt gtattgatgt ttttcatttg
    14641 gccaagcata caagccggca tttatcatgt tggtggattt gtaacgaaaa caggtgccat
    14701 cggtactttt gtttatggct tcatcttaag attgttaggt ccactcggtt tacaccatat
    14761 tttttactta ccgttttggc agacggcact tggtggtact ttagaagtca aagggcactt
    14821 agttcaaggt acgcagaaca tcttctttgc tcaacttggt gatccagatg tgacgaagta
    14881 ttattcaggt gtgtcacgct ttatgtcagg ccgttttatt acgatgatgt tcggcttatg
    14941 tggtgccgca cttgcaattt atcacacagc taaacctgaa cataaaaaag ttgtcggcgg
    15001 tttaatgtta tccgctgcac tcacttcatt tttaacaggt attaccgaac ctttagagtt
    15061 tagtttcttg tttgtcgcac ctattcttta tgtaatccat gccttctttg atggattagc
    15121 atttatgatg gcagacattt tcaacattac aattggtcaa accttcagtg gaggctttat
    15181 cgatttctta ctctttggtg tgctacaagg taatagtaaa acaaactacc tatacgtcat
    15241 acctattgga attgtgtggt tctgtttgta ttacatcgtt ttcagattct taattacgaa
    15301 atttaatttc aaaacacctg gtcgagaaga taaagctgca gcacaacaag ttgaggctac
    15361 tgaaagagca caaactattg ttgctggttt gggaggcaaa gataacattg aaatcgttga
    15421 ctgttgtgca acgagactac gcgtcacact tcatcaaaat gacaaagtcg ataaagtatt
    15481 actcgaaagt actggtgcca aaggtgtaat ccagcaaggc actggtgtgc aagtaattta
    15541 tgggcctcac gttacagtta tcaaaaatga aattgaagaa ttgctcgggg attaagacta
    15601 accgaaatat caacagaact aatggcaacg atgtacgaag taagaagtga catcgttgct
    15661 tttattttta atgttacatt tgaagcatta agttcatcat gcactgtagt gagcccgcaa
    15721 atcgcctctg ctagacaatc atcttaatgc tatgattaaa gcttaagtgc cagatttgaa
    15781 tttaatttca acaacgactt tcactacatt aaaaataggg ctactcgaca catatagttg
    15841 tatcaaatag ccctttatac aattttttgg gtaaggtttt acaatttttg ggatggtata
    15901 gattttataa aaagttattt aagttcttct gcttcagcca taatatcttt taatgtttta
    15961 gctgaatgtg cgaacttgct ttgttcttcg tcgtttaatg ggatttctac gacgttgcga
    16021 ataccgtttc tattgatgac tgctggaaca ccaatataaa catcttcttc ctcatattcg
    16081 ccttctaata atgctgatac agtcaatacg gcatcttcat ttctgaaaat cgcttcagta
    16141 attctagcta atcccattgc aacaccataa taagtggcac ctttagcttg aataatgtca
    16201 tatgctgcat cacgtgtttg aacaaaaatt tgttcaattt gcgctttgcc ctcaggacgt
    16261 tgttcaagta atgtcttcaa aggttgaccc gcaatattag cgtgtgacca tactggtaat
    16321 tcagtgtcac catgttcacc aataatttga gcatcgacgc tacgtggcgc aacatcgaac
    16381 gcttcgctta acaataatct aaagcgtgca gagtctaaaa ttgtaccaga acctataaca
    16441 cgttctttag gtaaaccaga gaatttccat gttgcatacg ctaaaatatc aacaggattt
    16501 gtagctacca agaaaatacc atcaaatttt gatgccatta cttcaccaac aattgatttg
    16561 aatattttca agtttttaga tactaaatct aaacgtgttt ctccaggttt ttgtgcagca
    16621 ccagcacaga tgacaactag atccgcatca tgacaatcac tgtattcgcc agctttcaca
    16681 cgaactgttg ttggagaata tggtgtggca tgttttaaat ccataacatc tcctcgaact
    16741 ttttcagtgt ctaaatcaat gatgactaat tcatcaacaa tgctttggtt cactaatgaa
    16801 aatgcgtagc ttgaacctac tgcaccatta cctattaata caactttgtt ccctttaaat
    16861 ttgttcatta caaaaactcc cttatgatta attcactaac atacatgtag cttcaaatat
    16921 gttagtttaa tgctgcttat tgacgataca aaagcaaata aacatctctt ttattttcaa
    16981 cgcataactt aaaaggtcat gtgtcatccg cttttaagtt tgtgatttat ttcacatata
    17041 aaatgtaaca tgcattaagt actgggtcaa tattaaattg tgatttattt cacattttat
    17101 tttaattttt acaccttttt aatttgtata cgattacatc ttagatgtct ttagtcttcg
    17161 tacttcgcca gtgattattt acactttcac atttttatta tcatgtttac ttttttctag
    17221 gaaaacaaca atgttttttg aattagtcaa ataaatgcgc tcaatcgtcg gtgtgcaaac
    17281 agacaattgt acacaatgct tattgataag tatttaaaaa attaaaaatg tcatacaatt
    17341 atcaaatttg ccattttatt tatattttct caaaccaatt aattgaatat cgaaattttt
    17401 agtagaataa tcaaaatata cagattaaag gaggagtatc atgcttacag aacaagagaa
    17461 agacattatc aaacaaacgg tgcctttact taaagagaaa gggacagaaa ttacgtcaat
    17521 cttttatcca aaaatgttta aagcgcatcc tgaactttta aacatgttta atcaaacgaa
    17581 ccaaaaacga ggcatgcaat cttcagcatt agcacaagct gtaatggccg cagcggttaa
    17641 tatcgataac ttaagtgtta ttaaaccagt cattatgcca gtcgcatata aacactgcgc
    17701 actacaagtt tatgctgaac attatccaat tgtggggaaa aatttattaa aagccattca
    17761 agacgtgaca ggattagaag aaaatgaccc tgtcattcaa gcttgggcaa aagcatatgg
    17821 cgttattgcg gatgtgttca tccaaattga aaaagaaatt tatgatcaaa tgatgtggat
    17881 tggttttaaa ccattcaaaa ttaccaatat taaacaagaa tctgaagaca ttaaatcatt
    17941 tacagttgaa actgaagaat atgactttag tgaatttaca ccaggccaat acatcacagt
    18001 tgatgtttct agtgataaac ttccatatag agctaaacgt cactattcta tcgtatcagg
    18061 tgaaaaaaac catttaactt ttggcgttaa acgtgatgtc acaacagaac atgaaggcga
    18121 agtttcaaca attttacacg atgagattaa agaaggcgat atgattaatt tagctgcgcc
    18181 tgtaggtgga ttcgtattag agaatacgac tgaaccacaa cttttcttag gttcaggtat
    18241 tggtgttaca cctttagtag ctatgtatga agctgcctct gccaaaggtt tagatacaca
    18301 gatggttcaa gttgctgaaa atgaacaaca tttacctttc aaagacaact tcaacagtat
    18361 cgcaagccat catgacaacg ctaaattata tacacactta aaagataaac aaggctatat
    18421 tggtgctgaa gaattacaag tatttttagc aaataaacct gaaatttata tctgtggtgg
    18481 tacaaaattc ttacaatcta tgatcgaagc acttaaatct ttaaattacg atatggatcg
    18541 cgtacactac gaaacattta ttccaagact aagcgttgca gtataactga gaaaagaggt
    18601 ttagtgctca tatgaaactt aatcaacgtt acgtaaaagt atttgcatta tatttcgtaa
    18661 gtattgttac tgcaaatatt attgttaaaa ataataattt aattaaaaca ttgatacaaa
    18721 ccatagccgg gtacacggtc tttgcagttg gtttgaagta tttaactaaa cgtaaaaata
    18781 aatgacatct ataccaaaac agctatgact ttactttgtc atggctgttt tttaattggg
    18841 agtaggacag agatgatatt ttcgcaaaat ttatttggtc gtcccaccac aacatgcatt
    18901 gatgtatgct cactgaattt cataagaaag gagttcacaa gatgaccgta gatattggac
    18961 ggatttatga caataaagat aataccgacg ctattcgtat cctagtcgat agagtctggc
    19021 cgagaggtat ttcgaaaaga actgctaacc tagattattg gttaaaagac attgcccctt
    19081 ctactgagtt gcgacaatgg ttccaacatg atcctaaact ttttggagct tttaaagaaa
    19141 aatatgaaaa agaattacgt gatcaggatg cgcaaaaaga tgcttttgaa aaattaaagg
    19201 atattgtaaa tcagcataat catgttctat tgttatatgc agcaaaagat actaaacata
    19261 accaagctgt agtactacag cagttgctca atacttagtt atatatgttt atcgtgaatc
    19321 actataaatg tcgctaactt catttctaat atttattttt aaaaagcata attactacaa
    19381 ttaattgaac tttaataatt actaaacttg aacaacattt tactttaaca aaataaagtt
    19441 ttaaaatatt aattgttggg ttttacatag taattagaaa gagagtgtta atgaaatgaa
    19501 gtttaaaaga ctagcaacta tattttcagc agtattagtg ttatcaggat gtggctcaat
    19561 gcattcatct ggcaaagact taaatatttc gttaccgtta aaaacaaagt ctattgcacc
    19621 ttatgaaaca gatgtaccag ttaagattgg tgctgcagaa tcattattta aaacaaatga
    19681 tcaaggaaaa atagaaaaag cattagtgaa atcgtatcat caaccaaatg atacaacgtt
    19741 agatatagaa ttaaaagata atattaaatt tcaaaacggt caaaaattga ctgcagaaaa
    19801 agtgaaatct agccttgaaa atagcatgaa aaaaagcgac ttggtcaaat attcattacc
    19861 aatatcatca attaccgcta aaggtcaaaa actgacaatt aaaaccaact ccgcttaccc
    19921 tgaacttgta tctgaattag ctaatccttt tatggcaatt tatgatacag atgctaaatc
    19981 agatgttaat caaactcctg ttggtacagg cccttaccaa ataaaagatt ataagcaatc
    20041 tcgaaaaata tcattgtcga attttaagga ctattggcaa ggtaaaccga aacttgatca
    20101 tattactgtg acgtaccaag aagacggcaa taatcgcgtc agaaatttag aatctcaaaa
    20161 agatgattta ataactgatg tcccagttaa taaagttcaa gacatagaaa ataatcaaaa
    20221 tttaaaagtg tcaaaagaat ctggatttag aacttcttta cttatgtata atcatactaa
    20281 taaaaaaatg actaaatccg ttcgtgaagc attagatcat atcattgata gacaaggtat
    20341 tgcagatcat atttatcaag gttatgcgaa acctgcaacg agtccattta atgataaaat
    20401 tccatatatt aaagaaccta agttaaccaa acaaaatatc gaacaagcaa aaatgttatt
    20461 agctaaagat ggttatacaa aagaacaccc tttaaaaatt aagttaatta cgtatgacgg
    20521 tcgtccagag ctatcgaaaa ttgcgcaagt attacaatca gatgctaaaa aagcaaatat
    20581 tgaaatcgac attaaaagtg ttgatgacat agaaggttac ttaaaagacc gttctgcatg
    20641 ggatgcaacg atgtatagtt tcggaacaat tcctcgtgga gatacaggtt atttctttaa
    20701 tcaagcatat aaaaaagatg gtgctattaa taaaggagac tacaataata gcaacgtcga
    20761 tgatttaatt aatcaattga atcatactgt agatgttaag gaacgtcaca atatttctaa
    20821 tgatattatt aaattatcaa gtcgagatgt accaaacagt tatattgctt acaacgatca
    20881 aatagtcgct gctaactcta aggttaaaaa ttataaagta acacctgaag gtatctattt
    20941 aatcgattat cgaacaacga ttgaacgata agggagtgtg ggacattgat ttatgtctca
    21001 gccctgcttc aactataaaa accacgccga agtcatccaa tatgattatg gcgtggttta
    21061 atttgcttat atgattgtat taatttctgt ttgatgaatt tcaataaaat gattattaca
    21121 tactttcagt gaatcatgtt cattttatat aaatctaatt gtcactattt gtttaacgtg
    21181 aaacactttt ttaactccaa aaagtattcc tattccactc ttttaaagac aaattataaa
    21241 aatagcttat gtcacaaaca tatgagcaac ataagcttta attattattc gtctactttt
    21301 ttctgttgtt ctttaaattc tttctgcagt aaattcactt gttttaaacc tgcccccatt
    21361 aaaatgaata caagtatcca cacaataacg attaaaataa atattttcaa cgtatcactg
    21421 ccaagtacag ttaaagatgt atcccaaatg ccatgtaaaa caacggctga taaaaagaat
    21481 attaaaaagc gtttatcaaa aatatctttg aattcaaagc catgctgttc tttcgcaata
    21541 actattgcag caccaacaat cgctgaccaa actaaatgac caccaatcgc agtccacgca
    21601 cgtgtgaaga caatatctaa taatgggaca ttttctccta aagcgaaatt caaaatataa
    21661 cctgctgatt caaaaactgc gaaccctgca ccaatagcag caccgattaa taatccattc
    21721 aaaatcttat ttgttttcaa tttattgacg aaataaacaa taatgagtgc ttttccagtt
    21781 tcttcaacta atcctactaa aaatgcatcg aaaaatgtta aagaaccaaa cctttcgaat
    21841 tgatcactaa aaacgacaaa tctatataat accatcgtac ttagtaatga aaatacgccg
    21901 ccaataaaga acatgataat aacttcaaaa atgctaatat ttttaaacgc atttgattca
    21961 taaaagaaga acaaacccga taatggtact gttaaagccc ctataaaaat gagacccgga
    22021 atcgcgttag tgttattaaa aattgctgcc atgacccata atccaataaa tgttactgtg
    22081 aaagcgatga atactcgaga aaagagccat ggcttccccc attcttctga tatgtcacaa
    22141 attgctggcg tagttttagc agtacccgct ataaatattt catctgcttc gtttttagta
    22201 tgcggcttaa atacttcaga aagcatatct tttaaattaa gatttaagct ttcatcattg
    22261 cccgctagtc taccgattga tttggttgca ttatcaaata aacttcttgc ttcgtttttt
    22321 aaatctgtat tttgcgctgt ttcatgcgct ttagcttcca tttgtttatc gatagattca
    22381 ttatgtatgt cggacgtctt agttgattgt gtacttgctg atgacatccc ctcttcacct
    22441 gtattaggtg caactgattg cgtttgtgtt gtctcttctt tttctgtatt tcttcgatga
    22501 ttaagacctt ccatagactt acctactttc acttttcata ataaaactat tttaatttaa
    22561 tattgatgga ttttccatat tttaatataa atactttgtt cgattgggca ctttgtattt
    22621 catatttaaa aatcaaaacc tatttaactt tttatattcc gaacaatatt tttatataag
    22681 tattttaatg atattcaatt cattacaaac ccttgatatt acaattgtta tcgaccgtca
    22741 aaatgtgtct atactgtgtc tattatcaga ataaaaagat atttctaatt gattttaaac
    22801 gtcgttatgt tatatttctt gtaagggttt acacaaagtg taaaaacgct atattttatc
    22861 aaatgtgatg gaggtgactt gcttgaaaca aatcacatgg cacgacttac aacatatcat
    22921 taaagatggt gatgtgattg gtttaccagc attagctgta gccaacttac ccgccgaagt
    22981 tctacgtgct gtgttagcgc aacatgacac atatcatacg cccaatgatt taacgtttat
    23041 attagcgaat gatattcata gtttaggtgc cgcaccggat ttagatgatt ttatagaacg
    23101 tcgcatgatt aaacgtgtca ttatgagtat tttaacggct tcttccaaaa cggcacaagc
    23161 aatgaaaaat aatgacattg aagcttattt tttaccacaa ggtatcattg caactcatta
    23221 tcgtcagagt aatcaattat tacctggagt tattactaaa atcggattaa acacagctgt
    23281 tgatcctaga tacggtggcg gtaaagtaaa tacacgaaca acggatgatt tagtttcatt
    23341 agtaaccatc aacgatgaaa catacttaca ttacacattc cctagcgttg atgtggcact
    23401 actgagagga acatacgcag atcaacaagg taacatttat ttaactcaag aagcgtactt
    23461 gagcgagtgt tatcatgtcg cattaaacgc gaaagccaat catgggaaag ttattgtaca
    23521 agttaaagct ttagttgacg actatcaact aaaaccgaat gaagttgtta tcccaggaaa
    23581 tcttgtcgat tatgtatacg tcacagaaga tgaaaagaat caccgccaag taattcaaag
    23641 tcattattta ccagccttgt ctggagaaga acgaattgat ggaatacctg aacccgcatt
    23701 accttttaat agtcgcaaat tgattctccg acgtgctgct cagtttttaa cttatggcga
    23761 tacaattagc atcggttatg gcatcaataa tgaactctct aatttattgc atgaagaatg
    23821 tgttgaacat gatgtgcaac cgattttaga tgttggcatt ttcggtggat tcgttgggag
    23881 tcgtgaacat tttggtatga attacaatgc agatgtgcgc atgcctcatg atcgagcatg
    23941 ggattttatt tataacaatg gtgtatcagt tgcctatctt agctttgctg aggttgatca
    24001 atacggcaat gtcaacgtgt cttacttcaa tgaccgacta aatggatgtg gtggctttat
    24061 agacattacg caatctgtaa ataaaattat cttttcaggt acttttgtag ctggcagtca
    24121 tgtctcatgc cataatcaac gattaaacat tgaaactgaa ggacaaaacc agaaatttgt
    24181 atcagatgtg agccatatcg actttaatgc acaatattca caatcactcg agcaagaaat
    24241 ctattttgtt actgatcgtg cagtattcga actcaccaat caaggcttga aactaattga
    24301 aattgcacca ggtcttgatt tgcataaaga tattttgaat caaatggctt ttaaaccaat
    24361 tattgctgat catttaaaat taattgatac cagcatttac aaagaaaaat ggggacaact
    24421 taaacaatca attcataaag tatgaaagtg aagctaaagg gggtatacta atgaatttcg
    24481 attggataaa aacgcgttca gacttcgatg atgacaagcc tgccgttatt gatcacgcaa
    24541 aacaaacatc ttggacatac caacaactca atgcacgcgc tgataatatg gcacattatt
    24601 taacatctca aggtgttaaa aaaggcgatg ttatcggtat ttttgcgcca aatgatattg
    24661 caatattaga tttattgttt gcttgtttta aaacaggtgc ggttttttta ccattgaatt
    24721 ggcggcttaa cccaaaagaa attgcagcca ttgtggaaga tgcacaatta aaactgctct
    24781 tctatgctga aaaacattta agttcactca ccgatattga ccaaaactta ttgcatatgg
    24841 atattgatgt ggcgcaatat gatgaaatcg taaatccaga ttatcaccaa ccttttcaag
    24901 caacacctgt tgaaccacaa gatcttgcag cattaattta tacaagtggt acaaccggat
    24961 cacctaaagg tgtgatgttc tcatatgaat catttgttca taacggtgca aacttagagc
    25021 tgacgtataa gttcaattca aactatatta cgattgtatc aacaccaatg ttccatgttt
    25081 tagggtttaa cgatactgta ttaccagtat taatgtcagg tggcacactt attcttcaac
    25141 gttactttaa cggcgaagaa ttgaatgaca tgattgcaca atatcaccct acatttatta
    25201 tcatgattcc gacaatgtat tatagtacgc tacgtgctag taattttaat cctgaaaatt
    25261 ttagagctat ggattatatc atccaaggtg gttcacaacc attaccaagt atccaagcag
    25321 cttttaaaca atatggcata aacattatta acggctatgg tttaactgaa gcacctcttg
    25381 tacttgttaa tacaccggaa aattcaaaac gtaagccaat gagtattggt aaagcagtca
    25441 tgttcgttga tgcacgtatc cttgatgata acggtgagga agtacctact ggtgagattg
    25501 gcgaacttgc aattaaggct aaaaatgtca cgccaggata ttggaataaa ccagcagaga
    25561 ctgccaaagc atttcatggt cgatatttat taactggtga cttagcgaag atggacaacg
    25621 atggcgatat atttattatt gaccgcaaaa aagaattaat cataactggt ggcgaaaatg
    25681 tcttaccatc cgaagtcgaa aatgctttag ctgagcatcc actagtagac cggtgtgtgg
    25741 tcgttggcta tgatcatcca aaatatggtg aatcaattgc tgcagccatt atacttcgcg
    25801 aagatgaacc tcattacgct gaaattttaa atcaacatat gcgaagtcgt ttagcaggtt
    25861 ataaagtccc aagaatgtat gtaccagtga cacatatgcc gttaaacagt acgcagaaac
    25921 cagataaact tgcgattcga caaatgatga atgacaaagt ctcgcaaaca ctttaaaggt
    25981 gataaaaatt tttgacattt agtgtaagcg tttacaaata aagcgtgttg tttttgaatt
    26041 aaatgcattt cacattagta ttcatattat ttttaggagg aatttatatg acatttgaaa
    26101 aagaaacggt cttaaaaaca ttatttcctg aagatgtact tagtattgct aaaggtttaa
    26161 cagacggtga agtcgaattt ttacaacaag tagattcatt gctagaaagt aagtaccgtg
    26221 aaaatattaa tcaacattgg atagacgcta ctgtacccga ggactatttt aaagatctgg
    26281 gagaattaaa ttattttaac aatccattac tttacaagga tcgtccaaac gccaaaatgc
    26341 ctagtcaact atttcagttt ttcatgtctt acctactcgc gcgatttgat atttccttag
    26401 ctaccctact cggtgttcac caaggtttag ggcataacac tttctatttc ggaggtagca
    26461 aagaacaaat tgcgaaatat gtacctaaat tacagtcaca tgaactgcgt acatgctttg
    26521 ctttaactga accagaacac ggttcggacg ttgcgggagg tcttgaaaca gtcgctgaac
    26581 gccaaggcga tacttgggtt atcaatggtg aaaagaaatg gattggtggt gcacatgtat
    26641 ctgatgtcat tccagtattc gcagtaaata aagaaactgg caaaccccat tgctttgtag
    26701 tcagaccaga acaagatggc gtcgatattg aagtcattga taataaaatc gcacttcgca
    26761 ttgttcctaa cgccctaatt aaattaacta atgtcaaagt agatgaagcg gatcgcttac
    26821 aaaacataac aagctttaaa gatattgcca aaattcttta ttcaacgaga gcaggcgttg
    26881 cttatatggc tacaggtggt atggctggcg ctttacgtgc cacattagat tatgtcactg
    26941 agcgtaagca attcggcaaa ccaattagta aatatcagtt aatacaagaa aagctagcaa
    27001 tgatgcaagg taatttagct caagcaatgg caacatgtgc tcaattagct aatatgcaag
    27061 cacatggtga atatgacgag gttgcaactt caacggcgaa gatgatgaat gccttacgtt
    27121 tgcgtgagac agtagctatg ggccgcggta ttacaggtgg taatggcata ctagctgacg
    27181 attatgatat tgcacgtttc ttctctgatg cagaagcgat ttacacgtac gaaggtacac
    27241 atgaaattaa tgccttagta attggacgcg ctttgactgg agattctgct ttcgtataaa
    27301 tagcaaataa ttatatgaga tgcattaatt tcactaaaaa agacttattt taagcataaa
    27361 gctttttcct taaataagag gctaagatga ctgtcaaaga tacttaatta attttataaa
    27421 atagcaacgt tattccaatt atcttaatgg ttatcttatc ctcaactaaa ttggaggaat
    27481 cactatgaca attaataaag taaccgttct tggcgcaggc acaatgggcg ctcaactggc
    27541 agcacttttt gtgaatgctg gacttaaagt aaaactatta gatattgtag tggacaaaaa
    27601 cgatccaaat ctcattgcga aaaaatctta cgataaaatt acagataaga aacggccgct
    27661 actattcgac ttaaatctag cgagtcattt aacatatggt aattttgatg atgacttggt
    27721 aaatgatgat gctgatttat atatcgaagc agtcaaagaa gatattgaaa ttaagcatgc
    27781 tgtttggcaa caagttctac aacatgctaa agaagatgct ttattcgcta caaatacatc
    27841 aggtattcca attaatgcga ttgctcaagc atttaacgag aaggatcaag aacgattctt
    27901 tggtctacat ttctttaacc caccacgtat tatgaaatta gtggagttaa tacctacgtc
    27961 acacacgaag gaatctatta tattagatgt aaaaaatttc gcgcaaaatg tgttaggtaa
    28021 aggtgtcatt gtcgtcaatg atgtgcctgg ctttgtcgca aatagagtcg gcacgcaaac
    28081 aatgaatgat attatgtatc gcgccgagca acacaagata agcattgtag atgtggatgc
    28141 tttaactggg caagcgattg gtcgtcctaa aacaggtaca tatgcgctat ctgacctagt
    28201 cggtttagat attgcagtgt ctgtaattaa aggcatgcaa caagtacctg aagaaacacc
    28261 ttattttcat gatgtcaaaa ttgtaaatac gttgtttgac aatggcgcac tcggacgtaa
    28321 aacgaaacaa ggattttaca aaaaggataa agaaactaaa gctcgacttg tttacgatgt
    28381 tgaaaaacaa gattatgtac ctgtatcgca accacaatta ccaattttaa atgaatttaa
    28441 taaagactta gtgcataacc ttgataccat attcaatgcg caagacgaag cgggactatt
    28501 tttatgggag acattacgta ataatttcta ttactctgct atcaatgtac ctaaagctac
    28561 cgatgatttc cgagacatag accgtgcgct tgtctggggg ttcaactgga aacttggtcc
    28621 attccaatta tgggatgcaa tgggatacga acgtgttaaa acacgtatgg aagacgaact
    28681 tggagactta ccacaatgga ttagtgattt agatggtggc ttttataaac aagatgagac
    28741 cattgaatat gcaacaccta tttctcactt cgtaaaagat gaactttggg ataaaggtga
    28801 tgccaaactt tccgtaactc atgatgatca actgttactg aaattacaaa gtaaaaataa
    28861 tgtcattacc gatgaattca acgatgcgtt agttgatgcg attgatttac tggaaaatga
    28921 ccattacaca agtatggtta tttatgcaga tggtaacaat ttcagtgtgg gtgctaacct
    28981 tttcttaatg aaaaaggcgc atgaagacgg tcttgtagat gatgtcgttg cacaatcaat
    29041 tgataaatta cattatagct ttaatcgttt gaagtatagt ttgaaaccag tagtcacagc
    29101 tgttcaaggt cgtgccttag gcggtggctg tgagcttgta ctttactcac ctattgttgt
    29161 cgctgcaagt gaaacatata tcggtcttgt tgaagcaggt gttggcttat taccgagtgg
    29221 cggtggcctt gcagaaatgg ctgatcgcat attacgcaca tcgcataagt ttgatgacaa
    29281 acaagcttcc atgacaaaag tactgacgaa tatcgcattt gcgaaagtct ctacaaatgc
    29341 ctttgaggca cgtcgttatg gttatttacg tgatacagat acgattattt tcaatacagc
    29401 acaacgtgtc gaagttgcgc tcaaacgtgc gaaatatgaa gcagaaacaa actatattcc
    29461 gaatcctaga catcaatata tcgctttagg tgaagacttc aaagcattga tccaaggaca
    29521 attagatgcg caaagacggg gtcattttat tagcgaccat gattatcata ttgccttaaa
    29581 tatcgccaca attttagcgg gtggtgattt accaagaaat acatttatca atcaacgtta
    29641 cattcaatcg ttggagaaaa ttggctttat tgacttacta aaatctaaaa aatcatatga
    29701 aagaattgca catatgttaa aaactggtaa gccattacgt aattaaaaga tagtcattaa
    29761 gagaggatga taaccatgca agaagcatac attgtagctt atgggcgttc agccgcagcg
    29821 aaagcaaagc aaggcgcatt attccacgaa agacctgatg atgtcgcagc caaagtatta
    29881 caaggcgtat tgaaacgtat tgacggaaaa ttcaataaga atatgattga agatgtcatt
    29941 gttggtacgg cttttccaga aggattacaa ggccaaaaca ttgcacgaac gattgcattg
    30001 cgtgcgggat tatctgacac ggtaccgggt caaacagtga atcgctactg ctcatcagga
    30061 ttacaaacca tcgcgattgc agccaatcaa attatggctg gtcaaggaga tatacttgta
    30121 gctggtggcg ttgaattgat gagtgccgta ccaatgggtg gcaacgagcc cacaaacaat
    30181 ccaaccttac aatatgatga tataggtgcg tcatatccta tgggtttaac tgctgaaaat
    30241 gtagcatccc aatttgacgt atcacgtgaa gatcaagatg cttatgctgt cagaagtcat
    30301 caacgtgcct atgacgcaca acgtgatggt cggttcaaag atgaaattat tccaatacaa
    30361 gtaaactcag ttgaatatac aaacgcagga ccaaaagtac acacaaatat ctttgaccaa
    30421 gatgaattta tacgccctga caccacgatg gaggcattag ccaaattacg tacagtattt
    30481 aaagctgacg gcactgtgac tgcaggaaca tctgccccac tttctgatgg tgcaggattt
    30541 gtagttttaa tgtctggaga taaagtgaaa gaactcggcg tgacacctat tgcacgattc
    30601 gttggtttta aggcagtagg cgttgacccg aaaattatgg gtattgggcc tgcatatgcg
    30661 attcctgaag tattgtcact cagcaatcta tctgttgaag acattgattt gatcgaattg
    30721 aacgaagcat ttgcttctca aacgattgca tctattaaag aagtaggtct agatatatca
    30781 cgtacgaatg tgaatggtgg cgctattgct ttaggtcatc cattaggtgc tacaggcgca
    30841 atgttaaccg cgcgtttact taatgaaatg ggtagacgtc ccgatagccg ttacggcatg
    30901 gttacgatgt gtattggtgt cggcatgggt gcagctgcta tatttgaata tgtgcgttag
    30961 aatggttgat tttggatgaa gcggattcgt tttgttattg aatgaagtag gctgaagttg
    31021 aagccagttg aagttgaagc gggttgaagc aatttcgttt tattaatgaa gctgtgtgaa
    31081 atatagtgat tgaacaaaaa agtggtttaa tgggatggtg gttatttccg ttttagaatt
    31141 taacatttac acgtctaatt ttaatcattg ttttaaattt tatgaatcga agccctttga
    31201 tttaataata tttgctaatg ctagtaactt atctgattgt tcatgtttaa aataaagaaa
    31261 accactcaca tcagtgtgtg ttcgaactag acttgtaagt tccagttcgg cacgactttc
    31321 taaagcaatt attattgctg tgattgtcgt atatcactta gatgtgcgtg gtttatttta
    31381 ataggttagt aatatattag gtcatgttat gtttaagact ataatgaata aataatttag
    31441 aaatatgctt ccgattgttc gatgctttaa ttcagttaga agcatcatag aatgcatgat
    31501 tactgttgta aagatacgta atgttttgta ttgactgtat gtctttggat agagttacaa
    31561 acttattttg ttactctagg cccatatgtc gcagtaccat ctgcatgtgt tgttacattg
    31621 tatgcatttg ttttacttgg cttcttgtat gtcgggcgag ctccgtatga cacttgaccg
    31681 tttgcatgtg ttgttacgtt gtatgcattt gttttgcttg gcttgttttg tgttgggcga
    31741 gcgccatatg atacttggcc gtttccatgt gttgttacgt tatatgcgtt tgttttgctt
    31801 ggcttgtttt gtgtcggacg agctccgtat gatacttgac catttgcatg tgttgttacg
    31861 ttatatgcat ttgtttctga tggcttattg aatcttggtc tcgcttcata tccaaatgtt
    31921 ccatcgttgt attcacggat acctgtacca gcatctctat atttaacata tttaggtgtt
    31981 ttgttaaatt gcggtctcgg accatattga gaagcttctg ttgtttcagt tgcttgaggt
    32041 ttaacttcaa tatcacttga ttctccttga gtacctttta acgttgattc agtaccttgt
    32101 ggttttattt caagtttaga tgagctacct tcaagacctt ctaaaatagg gttcgttaac
    32161 ggtgggtttg tataattatt gcttaatgat gggccgcttt gttccattgt tagaaaatcg
    32221 ggaccttgaa cgatttcacc ttgtaccgtt ttattttcca tcgttggata ttccggacct
    32281 tttacaattt cacctgtaat tgtgccctgt ggaattttaa ctaatggttg tgcaactggt
    32341 tgtgttgttt cttcagcttt accagccgta gttttaacct cttgttggtt atcaacttta
    32401 ggtgcttgag gttcttcaac tttcttctct tcttttacta ctggcgattt tgtttcagtt
    32461 tctccgtatt ttttgacagt tttctttttc caagaatcat ctgcttcttt aactgctttt
    32521 ttcgtttctt caactaattt atcaaaatta ggtttattat cactatttgt tttatagtta
    32581 tgtgttgtag gattatattt cgttatagat ttcggtctat tttgtttagt ttccataaag
    32641 aaatcatcaa taattgaatt taagtcatca atcatttctt ttttaatacg ttcatttgta
    32701 attttatgtg gattgtctgt atctccaagg attaagtcca gttttgctcg taactctttc
    32761 gcgtgctccc cataatcctt atcaccataa tatgatacaa ctaatgtatc aatttcagat
    32821 acgagatcgt atacttcctt agttgcttta tcttcttctg ctgcattaaa agttttcaag
    32881 tctgaattct tatccttaat atctttaact tctctgtgaa aatcatccag tgctctcttt
    32941 aatgcatcct gtagttcatt gtattctttc atcgaaagtt cttctaaatt atatttatga
    33001 aaattagcca tttttaaatc tgtacgagga ttttcttttt tataatttgc ataccattgt
    33061 ttataatctt catattgaga tttctttctc tccaaaagat attgatcttc ccttaatacc
    33121 ttttccaaca acctatcttt agcttcttta taaatattat ctccatattc atatttatta
    33181 gttaatccta tagcataaat tatatagtct tccaaataat atagagctga atttaaatat
    33241 ctgctatcta ttaatgtccc atttttactc ccagcattaa cttgtgattt cccactataa
    33301 tcctttgtta ctatcgcatc tgctttgtta tcccatgtaa ataagctaga tgcaactgct
    33361 aatgcgccta gcgaaattat ttgctttttc ataatttttt aattcctcca aaatgtaatt
    33421 gcccaatcta cattaaagaa acaaaatatt aaaagacatt aactatatat taactagaat
    33481 aaacaaagca ttaactatct ttttgttata gttaattagc tttgcaatat acaatagtgt
    33541 ataatttttt tgattaatgt acatcaaagg agtaacaaag catgacaaca caaatgaaaa
    33601 tcaaaacata tttagttgct ggtattaaag cggcgctcct tgatacgact ggtattaaat
    33661 tagcaagcaa atctgaaact acatcacata cgtatcaaca tcaagcgctt gtagatcaat
    33721 tacatgaatt aatagcaaac actgacttaa ataaattatc gtacctaaat ttagatgcgt
    33781 ttcaaaaacg cgatatttta gctgcgcact atattgcaaa atccgctata cgcactaaaa
    33841 atttggatca aatgactaaa gcgaaacaaa gattagaaag tatttacaat tcaatttcta
    33901 accctttgca ttcacaaaac aattaataat tcaccaataa atcatgtaag tgtttgtgac
    33961 gccaaattgc catacaaata cttgtcatat gaatataaac gaatgaaacg attgcctatc
    34021 ccatagatgg caacattaaa aagacctctg aaggtatcat cttgataact ttagaggtct
    34081 ttgtttatat tattcaaaca aaattcttat agaacgatcg aagtatgttt cgtctttctt
    34141 ttcttcttta atctgatcag ctaatgctgg gtcatctgtg ataataccat caacattggt
    34201 ttgtaagtat ttcgttaaat cttcttcgcc gttaatagtc caagtataga cttctttatt
    34261 ttccaagtgc gcttgattaa caagtcttgg cgaataagaa aaatcttcga tgacaaagaa
    34321 atctaatgat gtttctttaa aatgaccaaa ctgcaacgga atgatataac cacacttgag
    34381 atatggcgct tcttttttca acttagtcat cacatcataa tccaaagaca tcacacgata
    34441 ttgatgttca acaccatgct ttttcaaaat atcaataaca cgttgtgtat aatctgctgg
    34501 ttctttacca tgtggcttta actctactag tagcttcaca tttgattgtt tagccgtttc
    34561 aataaattcg tctaaggata caaattttgc ttcatgtcca ttttgacgca ttttcaaacc
    34621 gacgatatct ttgaaattag attcagaaat atttttatta acacctgtta aacgtttcaa
    34681 attgttatca tgactaacaa caaattgttt atctttcgtc ataattgtat ctaactcaac
    34741 gtattcgaca ttcgcttttg cagcagcttt caatgacgga atagaatttt caacgccttt
    34801 atcttcgaaa ccacgatggc caataatgga gatatttgta ttgatagtat tattgtaaag
    34861 taagtacatg ttataaccga taaaacatgt cactgcaagc accattgaaa ttataaagaa
    34921 cctagacttc cgtttcggtt ttggatattt aaattctaag cccggttggt ctaatacatt
    34981 ctcttgtttt aagtgcagta ctaacacact gattaatgat aatttcgtaa ataaataata
    35041 gaagaacaat gcgcttttca atacaacaaa taaaattgat gagactaaaa acttatctcc
    35101 ttcttcatct acacaaatag caagatatgt tgctcctgaa ataattaatg ttaaaatcgc
    35161 accaatgatg agttctaata taactatttc tataacaagc cgaaacttat ttcgcttcgt
    35221 aatttgccaa cttagtctca tatttttaaa taacgactgg cggtttaaaa tcgttaacgg
    35281 tagagtaaat attaatttaa aatttaatat aaatacagca atcataaagg taccgtaaat
    35341 gattatacct ttcgtcgttt tcataagttc ttccgttaaa aatttaggta tgtaaatatt
    35401 ttttgttaat actgaactta gtcctaggtt ggcaatgggt atcattaaca ttaaataaat
    35461 gacaaagaaa ataactggta cacctatgag tttacgcaca tttacaaagg catttttaaa
    35521 aatggattta aatgtaataa tctgtcgatc aaagccggca taaaccatat aaactaacaa
    35581 tgaaaactct acataaatca gaaaggcaac acttaatatg aatataataa gaagtatcac
    35641 actggcggga tgactaacga tttccgtcca attgttaatc gtaagttggc tttgcccagc
    35701 tacttttaac atcatattaa atagtaaaat taagtatgta ctactaataa aaatcatgat
    35761 taactgcaat agtaaggcat taatgctaaa acgcccttta ttttgataca gtaatttaaa
    35821 tactgcccat atatctttac taattctctt cataatcacg ctccgcattg ctttaatatt
    35881 aagtttcatc ttaatatttt tcattactca gggtcaataa aaatttgaaa agactcatat
    35941 tcatatgcaa gtagcaaata ataacccatt caacatcagc taaatgatga tattggaacc
    36001 ccacctttaa caagacatca cattctttat cagcatagct acttacaaaa acgtcttcct
    36061 tcaaccattg tagaagttgt ctcatatact tactacgtct tgctaatact caatactcaa
    36121 ttgaaaaaga agcatatgcc ccttcactct tgaagttgca tatgctttct tttcggtctg
    36181 aattgtattt ataattcaac gggaattttc cctttgaagt taacataacg gtaggctgct
    36241 ttaacagctt catcatcggg cgcttcgaca tcttctaatt catatgcaat gcccaatgtt
    36301 ttccacttat gaacacctaa ctgatgatat ggcagaattt caaacttttc gacgttatca
    36361 agagaattaa taaattcccc tagtttaatt aaatcgtctt tatcatcaga ataaccaggc
    36421 acaaggacat gtcgaatcca tacaggttgt ttcatatctg acagtttgcg cgcgaagtta
    36481 aggatgtgtg tattaggctt tcctgtcaat ctaatatgtt tgtcattatc aatatgtttt
    36541 atatctaata atatcaagtc tgtatgtttt tgtaattctt caaaatgcct ttgaaatgct
    36601 tttgtatcat tagcacatcc agccgatgtg tctaagcaag tgtgcacacc attttctttt
    36661 aattctgcaa ataatttttc taagaatggc atttgtaaca atggttcgcc accactgact
    36721 gttacaccgc cacccgatgc atcaaagtat ggtttgtatg gtaatatttc attcaccatt
    36781 tcatcaactg tgacttctct tgatggctca ctaattttcc aagtatctgg attgtggcaa
    36841 tacaagcatc taagtaagca tccttgtgta aataatatat atcttaatcc cggtccatcg
    36901 acagtaccta aactttcgac agaatgtaag tgtcccttaa gcatagtgct cccaccttaa
    36961 attttgttac atactttcat ggaatgtacg agaaattaca tctaattgtt gttcacgtgt
    37021 taatttaatg aagttaacag cgtaaccaga tacacggatt gttaactgtg gatattcttc
    37081 tggatgttcc attgcatcta ttaatgtttc acggttaaat acgttaatat ttaagtggtg
    37141 accacattgc attgcgtaac catctaacat actagttaag ttacggtttt gatcttctgg
    37201 ttctttacct aatgattttg gtacgatact gaatgtattt gaaataccat ctttacagca
    37261 atcgtaaggg atcttagcta cagaacttaa tgaagataat gcaccttttt ggtcacggcc
    37321 atgcattggg tttgcacctg gagcaaatgg ttcgccagct ttacgtccgt ctggtgtgtt
    37381 accagttttc ttaccgtata caacgtttga agtaattgtt aatacactca ttgtatgttc
    37441 tgaatcacga tatgttttat gactacgtaa tttagtcatg aagcgttcta ctaaatcaac
    37501 tgcaatatca tctacacggt cgtcattgtt accgtattta gggaagtcgc cttcgatttc
    37561 aaagtctact acaagacctt cttcgttacg aattggttta acttgtgcat atttaattgc
    37621 agataatgag tcagctgcta ctgataaacc agcgatacct gttgccattg tacgtacaat
    37681 ttctgtatca tgtaatgcca tttcaatacg ttcatagctg tatttatcgt gcatgtagtg
    37741 aataacattt aatgagttaa tgtaaacacc tgctagccaa tccatcattt gatcaaattt
    37801 cttgaatact tcgtcatatt ctaatacttc gctgttaata ccttcgaagt ttggaccaac
    37861 ttgtgcacca gatttttcat ctttaccacc attgatagcg taaagtaatg ttttagctaa
    37921 gttcgcacgt gcaccgaaga attgcatttg tttaccaatt gtcatcgctg atacacaaca
    37981 tgcgatacca tagtcatcgc catagctttc acgcataatg tcatcatttt catattggat
    38041 agaacttgtt ttaatactca tttttgcaca gtatgttttg aagttgtcag gtaaacgtac
    38101 tgaccataat actgttaagt ttggttctgg agctggacct aagttatcta atgagtgtaa
    38161 gaaacggaat gagtttttcg taacaagtgg acgtccgtca atacctacac caccgataga
    38221 ttcagttacc caagttgggt ctccagagaa taattcattg taatcaggtg tacgagcaaa
    38281 tttaacaata cgtaatttca tgatgaagtg gtcaataatt tcttgaactt cgctttcagt
    38341 aataacgcct gctttaaggt cacgttcagc atagatatct aagaatgttg atgtacgacc
    38401 taaactcatt gctgcaccgt tttgttcttt aattgcagca aggtatgcta agtataacca
    38461 ttgaactgct tctttgaagt tttctgctgg acggcttaaa tcgaaaccat atttttgtcc
    38521 aagttctttt aattctttta atgcacgata ttgttctgat aattcttcac gtaaacgaat
    38581 tacatcttct gacatttctg tagacatcgt gttgaagtcg tgcatttttt cttccattaa
    38641 gaaatctaca ccatataaag ctacacgacg atagtcaccg ataatacgtc cacgtccgta
    38701 tgcatcaggt aaaccagtga ttacacctgc tttacggcag ttcaacattt ctctagaata
    38761 tgcatcgaat acaccttggt tatgtgtttt acgataatct gtaaagattt tttcagtttc
    38821 ttcgtctaat tcgtaaccgt aagcttcaca agctgctttc gccatacgaa taccaccgaa
    38881 tggttgcatt gaacgtttga atggcttttc agtttgtaca cctacaattg tttctaaatc
    38941 tttgtctaaa taaccagcat catgagatgt gattgttgaa gctactttcg tgtccatatc
    39001 ccacatgccg ccacgttcac gttcttcttt cgataactgc attacttgtt cccaaagttt
    39061 agaagttgct tctgttggtc ctgctaaaaa tgaatcatta ccttcataaa gagtgtagtt
    39121 taattggata aactctctta catctacgtg tctgttccat cttccatttt taaatccttg
    39181 ccaagctgtt gcatgatttt tatttgtttc taacatacta aacgcctcca tctgtataat
    39241 gttgtgaatt tgaaatgcta tgttaacgtt ttcatagtca ttgtagcatg tttgttgtga
    39301 tttttttcac ataaattatg tacaattttc atcaaatgta tgtcaaaaca tagaaactgt
    39361 actaatttgt gtaacagtaa tatgctaatt gcattctgtt acacaacagt tataatgttc
    39421 actattttca ttgctttaaa agttaattta ggagtatata tttgtttata aaaaatgatt
    39481 gttgataagc gttaaatcaa cacttagaaa gatttacttt tatatatgaa gagactggat
    39541 taaatacttt tattgacgta aaaattcact tttgaaccgt tcaatatctt gccgattttt
    39601 atataacagc tacaaataaa atataacagt ttgattttac agcctcggta aatcgtcttg
    39661 acaaacaaaa atgttgtgct atcacaacat ttgcaacgtc ttaacaagtc atctataaac
    39721 atttctaaat atttaacatt acttatgcgt catttattgc taaaattatt gtattaaaat
    39781 atacatagaa ttgatgggat atcatgaaat caaaaattta tatcttgcta ttatttctca
    39841 tttttttatc agcatgcgct aatacgcgtc actctgaatc cgataaaaat gtattaacag
    39901 tttattctcc gtatcaatca aacttgattc gtccaatttt aaatgaattt gaaaaacaag
    39961 agcatgtcaa aattgaaatt aaacacggat ctactcaagt actgctttca aacttgcata
    40021 acgaagattt ttcggagcgt ggtgatgtct ttatgggtgg tgtgttgtca gaaacaattg
    40081 atcatccaga agattttgtt ccctatcaag atacatctgt aacacagcaa ttagaggatt
    40141 atcgctcgaa caataaatat gttactagtt ttctattaat gccaacagtt atagtagtga
    40201 attcagattt acaaggagat attaagattc gaggttatca agatttatta caacctatac
    40261 ttaaaggtaa aattgcgtac tcaaatccaa atacaacaac gacaggctat caacatatgc
    40321 gtgctattta tagcatgcat catcgagtaa gtgatgtgca tcaattccaa aaccatgcga
    40381 tgcaactgtc aaagacgtct aaagtcattg aagatgttgc aaaaggtaaa tattacgcag
    40441 gtctaagcta cgaacaagat gcacgcacat ggaaaaacaa aggttatcct gtatcaatcg
    40501 tttatccaat tgaaggaaca atgttaaatg ttgatggtat tgctttagtt aaaaatgcac
    40561 accctcatcc taagcgtaaa aagttagtgc aatatttaac aagccgctca gtacaacaac
    40621 gattagttgc agagttcgat gccaagtcaa ttcgaaagga tgttagtgaa caaagcgatc
    40681 agtctatcga aaatcttaag aacatacctt taataccgaa atctaaatta ccggatattc
    40741 cacatcataa atttttggag atgattcaat gacggcatac aaaccttata gacatcaatt
    40801 aagacgatcg ttatttgcgt ctactatttt tccagtattt ttagtcatta ttattggact
    40861 agtaagcttc tatgccatct atatttggat tgagcatcgt acgattcatc aacatgttga
    40921 tgaaagtcag tcatctttac atcatactga aaagcagatt cagacattta ttacacagca
    40981 taataattcg tttcaagagc tcgatttaac aaatcatcat gatgtgaccg ctacaaaacg
    41041 tgaattatta aaattaatac accaacagcc cgcaacactt tattatgaat tatctggacc
    41101 aaatcagttt ataaccaata attatgaaca tttaaacact aaaaatatgt atttattttc
    41161 aacgcatcag cttaaattca aaaatagtac atatatgctt aaaatttata tggctaacac
    41221 cccacgttta agtgaaatta agaaagataa ccgacaattc gcacttatcg ttgatcaata
    41281 tgacaatatt ttatacgcca acgatgaccg ttttactata ggtgaaaagt atagaccaca
    41341 acaattcgga tttatgaatg aatctgtaaa attaaaccat gctgaccatc gtctcattat
    41401 ttataaagat atacatgaaa acattgaaga tggtatcacg ttgttaattg ttatggccgt
    41461 agttcttgtt ttactagtaa tatttggttt cattagcgca gataatatgg ctaaacgaca
    41521 aacaaaagat atcgaaacga ttattcaaaa aatttactat gccaaaaatc gtcatctagg
    41581 tacatatacg cctttaaaaa acaacagtga actagaagaa attaataatt atatttacga
    41641 tttatttgaa tcaaatgagc aactgatcca ttctatcgag catacagaac gtcgcttgcg
    41701 tgatattcag ttaaaggaaa tagaacgcca attccagcct catttcctat tcaatacgat
    41761 gcaaacaata caatatttaa ttacgttatc tccgaaatta gcacaaactg tggtccaaca
    41821 attatcgcaa atgttacgtt actcattacg tacaaactca catacagtgg aattaaacga
    41881 agagttgaac tacattgaac agtatgttgc aatacaaaac atccgttttg atgacatgat
    41941 taagctccat attgaaagtt ctgaagaagc gcgtcaccag acaattggta aaatgatgct
    42001 tcagccactc atagaaaatg ctattaaaca tggtagagat actgagtcat tggacattac
    42061 tatacgcctg acattggcac gtcagaattt acatgtttta gtttgcgaca atggtatcgg
    42121 gatgtcgtca tctcgcctgc aatatgtacg ccaatctctt aataatgatg tatttgatac
    42181 taagcatttg ggattaaatc atttacacaa taaagcaatg attcaatatg gttcacatgc
    42241 acgactccat attttttcaa agcgtaatca aggtacgtta atttgttata aaataccact
    42301 ttcaaggggg aatgtagatg tttaaggtag ttatttgtga tgatgaacga attattcgag
    42361 aaggattaaa gcaaatcatt ccttggggag actatcattt caatacaata tacactgcta
    42421 aagatggtgt tgaagcatta tcactaattc aacaacatca gccagaatta gtaattactg
    42481 atattcgtat gccacgtaaa aatggcgtcg atttactcaa tgatattgcg catctagatt
    42541 gtaatgtcat catattatcg agttacgatg actttgaata tatgaaagcc ggcattcaac
    42601 atcatgttct tgattattta ttaaaaccag tagaccatgc acaattagaa gttattttag
    42661 gaagacttgt ccgtacctta ttagagcaac aatcacagaa cggccgtagc ttagcatctt
    42721 gtcatgatgc cttccaacca ctattaaaag tagaatacga tgattattat gtaaaccaga
    42781 ttgtggatca aattaagcaa tcttatcaaa cgaaagtgac tgtttcagat ttaattcaac
    42841 atatcgatgt tagtgaatcg tatgcgatgc gaacatttaa agatcatgtt ggtattacaa
    42901 ttgtcgatta tttaaatcgc tatcgtattt tacaatctct gcaattgtta gatcgacatt
    42961 acaaacacta tgaaattgca gacaaagtag gcttttcaga gtataaaatg tttagctatc
    43021 attttaaaaa atatttacaa atgtcgccaa gtgattattg taagcaagca aaatagatta
    43081 gcacataact aatgattatg gcgcctccat tttgttctat gaaaaagcgg actgatagag
    43141 aagttttcac ttttctttca gtccgctttt tagagagttt aaatataaaa caatactccc
    43201 tacaatattg acaccgctat atgtctttat tgtaaggagt aaattcattg agtcacccta
    43261 agtcgggcaa ataaggatac ttcataacag ggaattatga tctgccccga gacatagaga
    43321 tgatatattt gcaagggtag aagcagtatc tttcatctct tcacgcacat gccgtcgggg
    43381 caggattgcc aactatattg caatagattg gcgatacttt taatccgatt tatattaaat
    43441 ttttaaactt ctaattttct tttcttcata gaaagcaacg attcctaata gaatcatgcc
    43501 taggaataat gcaacataga agacgatgaa aacatctgtc catccactta atgtatatcc
    43561 aaagatgttt aaaccgttac gtgttggatc agcaatagcc gccaaaccaa ctttcgccat
    43621 tgagtcaccg aatagatacg cgaatgaacc tgtcattccg tttgctacac tgatggcatt
    43681 tttaggaaca aaaccagtca atgatacacc aattaataat tgcggaccaa agattaacgc
    43741 acctaatgca aacaatgaaa tgttaaccat catgacactt gtagcatttg tgtagaataa
    43801 gacaacaaat gtaatcataa acatacagcc aatagctaca attgcacgac gaccttttaa
    43861 taagtctgat acgtagcccc ataataaact tgcaactaat gcaccaattt caaagtagaa
    43921 tatcgtatta actgcatcgc ctttactaaa gtgtaaatgc tctgacacat ataacggtgc
    43981 ccagttatca ataccgattc gtacaatgta tacaaagacg tttgaaacac atagaatcca
    44041 tataacagga tttcccagga tatatttttt aaagatctcc catttcgtca taccttgaga
    44101 atcaatattt tctttatcga ccggctcttc ccaaatttct tcagcacgat tccatcctaa
    44161 ttcttccgga tcatcttttc cgataaataa tgttgcgata ccaataagta atgcaatcac
    44221 cgatgggaaa atgaacatcc ctataacatt tccatggaag aatacattag caccccaaag
    44281 tgcaacacca cctgctatgg caccaccgat attatgtgat gtattccaga atcctaagta
    44341 tcggccacgt ttcgttcttg gcgcccatct tgaaatcgtt gaataacttg caggtccacc
    44401 aactgattgg aacaccccgt taagtcccca aagtacaatt aataatccca ttacagaacc
    44461 aaagtaactt aaaacaaatc ccataattaa aactgtaatc gcagataaga taagtaagaa
    44521 cgagataata cgttttgtgt tacgtccatc gacaaaatat ccaagtaatg tttttcctaa
    44581 accgtacgtg atactaaatg ctaatccgat ataaccaagt tctaatgtag ataatccaat
    44641 ttcctctttt aaaaacggtt gtgccgcctt aaagttgttt cgaattaaat acatagccat
    44701 ataaacaaag aacactacga agaaagcttg catgaagttt ctaagccata atttacgttg
    44761 taccgataat ggaatgcctt tgttcggaat cttatggata tcaaaaaagt tcatctctgt
    44821 cacctcaatc attttcgtta agccaaatat atcacgtcgg tgtaagcgct taatatcctt
    44881 aaaattatta aattatccaa aatactgaac ataaacacat acttagaaac atatttatgt
    44941 actaaaatta ataaatagtt aatagtattt gacaagcttt atctataaaa tactttatgt
    45001 tgtcaaaatc aacgcttata catatgacat aatgacggag gacatcatga ttctatctat
    45061 attacttatc tttttctgta tcagacttgt cagcttaaag atatctatta atcactcaaa
    45121 acaattaaaa gcagacggtg cagttgaata tggcgttaaa aattcgaagt ttttagcaat
    45181 aacacacgtt ttaatttatg tattggctgg tgtagaggca tttattaata aagatacatt
    45241 tagctttgca aatggtattg gcttagttat attaatcttt gcttatatca tgttatttat
    45301 ggttattaaa actttaggtg gtatttggac attaaaatta ttcattttac ctaaccatcc
    45361 tattattaaa tctggattat ataaaattac aaaacaccca aattacttct taaacatcat
    45421 tcctgaatta atcggtgtat tattattaac acacgcaaca tatacaacta ttttattagt
    45481 accgtatgcg tatttcttat atgtacgtat taaacaagaa gagaaattaa tgaatattta
    45541 aaaagatgaa agcctgtcat cactttagtc tacatcgacg tccatcttta aagtgcattc
    45601 tgaaatatta tattcagaaa cctttaaagt atgtcgtgtc tatgaccatt gtgacaacag
    45661 gctttttttt attatcatat acctcactat tgaaaaggtt atatactctt ttcaatggtg
    45721 aggattgtta ctgtgaatgg gtatgaccat cgctatatac ttatataaag tacaccttct
    45781 ttacaaacaa tgacttgatt taaggtgtga aatttttggc aatggcttgg cgacatatgc
    45841 atttattcaa cactgccacc agtcttactt cagacaaact tcagactaac ctcgagttcg
    45901 tattatgccc accacatgtc tgctggcttg tcgtaaatat taacagattt caaagtttga
    45961 caagcttttt ggaaaccttc ttctactgac ataataggat cttcatgttc aatacttaat
    46021 acataatcat atccattaat aataagttga cttatgatat ctgcccatac atatggacta
    46081 tgtccataac caactgtacg gaatgtccat gctcttgtcg caacgttacc atatggttgc
    46141 atatcagtta gaccatacat atttacattt tcttgattaa tatacgtatc tttagcatgg
    46201 aagtgatgaa ttgcatttgc ttggcctaat atgcgaatcg cagcaattgg gtcaatacct
    46261 tgccaccata gatgactagg atctaagtta gcaccgatat attcatttgt agcctcacgt
    46321 aacttcaaca ttgtatatgg tgtatgcact aaaaatcctg catgcaactc tatggcaatt
    46381 tttacatctt gctcttttgc aaactcagct aaatcttgcc aatatggtat caacttttca
    46441 ttccactgat aatcataaat ttcagagtag gctgttggcc aaggtgtaac aggccaatta
    46501 ggctttttag cggtatcatc tgatcctgca atgccagaaa atgtattaac aactggcacg
    46561 tctaatagat ttgctaaacg gattgtttta cgtaacgttt catcggcttc tttcgcttct
    46621 atcggatctg gagaaattgg attgttatga caactgaaac cacttatttg taagcctctg
    46681 tctgtgattg acttcataaa tgcttggcgc ttgtcttcat tttctaacaa ctcatctaac
    46741 ttacaaaatt tatctcctgg gttaccacct gttccaactt caatcatatc caatccagat
    46801 tctgagacat aatctaacat atcttcaaaa tttttatcgt aaaataatac tgaaaataca
    46861 cctattttca ttgaaaagca ctccttcttt ttatattgag aattttgtat catatatgat
    46921 aatcattaaa gttgtatgct cttgttatca agatcgctac gataaatcgc ttctataagg
    46981 gcatttacat tgcgtgcttc ttccggtttc accacaatct cttctatccc taaacacgca
    47041 ttgacaaagt tacgcgcctg tctctcacca gcaatgtctt cgttatgctc aacattagct
    47101 ttgctttcaa aaatagttcc aaagcggggc tcatatattt caaatggaaa taaattgata
    47161 ccgccatctt ctcctgataa actaacgtga accttatctt ctttgatatt tgcagaccac
    47221 gaacattcaa actgcatgct tgctcgattg gcaaatgtca tataactagt aacatgatca
    47281 tcgacatcaa atttagtatg atcaaatgtt ccccaatcat taatttgatt cggttgtttg
    47341 ctcaattgat tatatgtttt tcctagcact tcatgcggca ccatatcttt acctagtagc
    47401 cacaaagata agtctaacaa gtggcaacca taatcgatta aactaccgcc accttgcaac
    47461 gctttattgg taaaaacacc ccagccaggc actttacgcc tacgcatcgc ttgtacacgt
    47521 gctactaaag gtttaccaac cacacctgat tcaattgctt ttttagcagt aattgccaca
    47581 tctgtgtgac gataatgata tgcgacagtt aataatttgt gatttttatt agccgcttca
    47641 atcatgcgat cacactcttc cgtcgtcatc gccattggct tttcacacaa tacatggaca
    47701 ccatggttca atgcttctat agaaagatca gcatggaatt tattaggtgt acaaatgacc
    47761 accgcatcaa caagtttaaa cagctcgcta ggtgtctcaa ctgcatgagg tatattaaag
    47821 cgcttcgcaa catcaatcat ctgcactgta ttaatatctt gtactgcaac taatgagact
    47881 gtgtctttga gtttcagcaa tgctggaata tgacggtctt gtgcaatacc accaacacct
    47941 atcacaccaa cttttaattt tgtcatgatg tgcctcctta ccgtatgatg ttattcaaag
    48001 taaattgctt tgcctgattt tgcagactga taaatcgctt caagaatttt tgtaactacc
    48061 attgcttgtt ccggtttcac aactggttca gtatcattta caactgcatc aatccaagct
    48121 tttgcttctt cttcagcttc atccacttca ttaccttcat aaaagtcgac gcctttgttt
    48181 tccaattcaa cgtgtttggt ataaagtgta cccatgtctt caccatgaat acgtagacca
    48241 tctttcatat cagcacctgc tttagttcct gataatgaac attttgcctc atccacttct
    48301 aaagaattaa tcgcccaagc ggattctaaa atgatcgtcg ctccattctt cattttaata
    48361 aatccaaacg cagaatcttc aactgtaaat tcatctggat tccatgaacc ccaagcgttt
    48421 gccgcatgat gctgtttatt taatttatgg aatgttgaac ccatcactga ttctggttca
    48481 taattatcca tcatccataa cgttaaatct aaagcgtgtg taccgatatc gattaatggt
    48541 cctccacctt gagcttcttc gtctagaaag acaccccatg ttggtactgc tcgacgacga
    48601 atggcatgtg cctttccgaa gtaaatgtct cctaagtcgc cacgttgcgc tgcttgatgt
    48661 aaaaattgac tatctgctcg gaaacgattt tgataaccta ttgttaattt tttacctgtt
    48721 gatttagctg tatctatcat tttttgagct tctgctgtcg ttttagccat tggtttttca
    48781 cacatcacat gtttaccagc atgcaaccct gctacagtaa tttcacaatg cgagtcattt
    48841 ggcgtacaaa catggataac atcaatcgtg tcatctttta acaatgcttt gtaatcatca
    48901 taaacctttg cattgtcagt tccgtatgct tctgccgcac tcgctgcttt cgaaatgtct
    48961 acgtcacaaa atgcgatcat ttcaacattt tcaacttttt gtaaacttgg catgtgcttg
    49021 ccattcgcaa taccaccaca cccaatgatt ccaactttaa tcgtcatcat aacgtcctcc
    49081 ttttaatctt tatcgtcatt agtggttctt tatcaattct gccccactcc taatttcaaa
    49141 ctaacctttt gtcgcacctg ttgttaaacc tgatactaaa tagcgttgca agaacaagaa
    49201 tacgattgct ataggtactg caatcataat tgcccctgct gcaaacactg tgaaattatt
    49261 tgcatactta tcattaataa agttgaacaa tccaactgct aatgtgaatt tttcaggact
    49321 tcttaatagt attttaggta atataaagtc cataaatggc cccataaaat tgaacaaagc
    49381 aacaactgct aaaatcggct tagctaatgg aagcataatt tgtaagaaaa tacgcatatg
    49441 ccctgcacca tcaattttgg cagattcatc aagttctttt ggaatcgtat cgaagtaacc
    49501 tttcactaaa aaggcattca tcggtattga tccaccaata tataccagtg ttagtccaaa
    49561 taaagaatct aataatccaa ttgtatttag caaaatatag attgcgacca ttgccattaa
    49621 tacagggaac atttgtaaaa tcaaaaatgt aatcagcccg tatttacgac caacaaagcg
    49681 atatctagaa aaagcatatg ctgttaacgt gacaaatatc acactaaaca gtgcatttgc
    49741 agatgctacg ataagcgtat ttttatacca agtcaggtat tgactactgt catcgaatag
    49801 taagaatgca taatttttaa atgttgcatt gtctggtatc attttggcac catacaagtt
    49861 cgtacctgga ttaagggaaa tgccaaatgt ccacagtagt ggatataaaa tgatgacaaa
    49921 catcatcgct ataaaactgt aaataccgat tgcttttaat atgtttttct tctttgtcat
    49981 cttataaacc tccctcatct ttaaacgtac ttgtacgtct gaattgaata aatgcgacaa
    50041 tagcaacaat aaatccaata attaatgaca caactgcacc catgttgaag ttgttaaact
    50101 caaatgtcag attatacacc caagatatca agatatctgt actaccagca ttctgccctg
    50161 acactggtgg accgccttta ttaaatagat aaataagatt aaaattattg aaattacctg
    50221 catattgcat aattaacaat ggcgctgtgg cgtaaatgac atgcgggaat gtgatgtttc
    50281 taaacttttg ccaactagac gcaccatcca tatctgctgc ttcgtaccaa tctgatgaaa
    50341 tactttgcag tactccagtg aacagtgcaa agacaaatgg gaatccaagc catacttgaa
    50401 tgccgattaa tgccactttt gcccaaaacg gatcacttaa ccatgctggt gctacaccta
    50461 ataaaggttg caaaatatca ttatttatcg caccaaattc atcattaaat aacgctacaa
    50521 atattaaaat tgtcacaaat gatggtacag cccaaggtag gattaacaca gtacggataa
    50581 atttcttacc tttgacgaca gggtgattta caataattgc caaaaacagc cctaatgcaa
    50641 tttgaagtgt cgttgcaaca agcgtccata ctaatgtcca agtaataaca ctgaaaaatg
    50701 ttttacgcca aacgccaatt gtgaataacg ttttaaagtt atctaaacca acccattcta
    50761 atgtgtgtct cggaggcgcg ttgtataaat tgtaatttgt aaatgctact ccaaacataa
    50821 atattaatgg aaatacaact acaaatatca ataagaatgt acctggtgag attagtaagt
    50881 atgggaacgt cttgtcccat gttgccacca tacgcgcctt cggatccttt atttcctcat
    50941 tgcgattaaa tcgttcagca ttacgatatg catctaaaat attaatgata tatagcatga
    51001 ttgcgaaagc aacgagtaag atagaaataa taccttgtgc aagtaagaca cgagaatcgt
    51061 ctaacttagg tactgtccct aatgtgaaca atccccaaaa accaatattt aaaaagctat
    51121 aaaaaacaga aataaaactg atgaaaaaga taaaaaatat cgtcccttta atgggtcttt
    51181 tattataaaa ctgtcccaaa ccaggtataa cagataataa tgccgctaat ttagggttac
    51241 gtttcgtcat aactaatctc ctttcttatc attttgtgat ggatgaagaa tcttaatatt
    51301 ttgcgttata tcattcgtcg cctcatctaa cgcttgttta ggattcttac catttgaaat
    51361 aaatatgctt gcattgccca tcggttccca aacttgtcgc atttcaggaa tattaggcat
    51421 cggttcagca tgacgtgctt gcttttcaaa cacttttaaa tttggattag atgatttcac
    51481 gtcaacacgt ccagtaattt cgctcatttc atctgtatat ttttgtaatg tatctttact
    51541 agtgatatac agcattaaat ctttagccca atacttatgt ttactatatt cagataaata
    51601 ccaaccacgt acacctagaa atggtttcat aggtttgcca ccatctgtag gtaatgttgt
    51661 tactcctaaa tctttaccaa acgtttcttg atattcatta atgttccacg gtccagtgac
    51721 aaattgtcct acttttcctt ctttaaaaag accaatcatg acatcatgtg ttgctgcctt
    51781 aggaagatac cctttgtcgt accatttttg taatcgttca gcattcttga cgacatgttt
    51841 tgaatttagt cctagctgat gaatatcata ttcactgcca tttttcttga aaatataatc
    51901 atcattgccg aataaaaacg gataattaaa atagaaattt ttagcatcaa ataacatacc
    51961 gtattgtttc tttttactat cagttagttt ggcagcatta gcttcaactt cttctaacgt
    52021 ttgcggtgca tttttcacta attttttatt ataaaaaagt gcggttgttt caacgatagc
    52081 tggcaatgct agttgcttat tgtcataatt catcgcttta agtgcttgct tattgaaacc
    52141 tttcaactca tcttttgata atttgatttc agcagctaag ccttgtagat aggcacttcc
    52201 agtattatca tgtgctaaga aaaagatatc tggacctttt cctgcaggag cgtctagcga
    52261 aatattttct agttgatcat tttgaccaat atttacaagc tttactttga tgccagtttt
    52321 tttagtatat tgatccgtaa tttttttata aaacgccatt tgcttgtcgc catccaccca
    52381 catcgtaagt tggttaggct tgtctttaga attatcttta ttcaatgctt tatcaatatc
    52441 ttctttcgaa cgattagggc cacatgcagt tacgattaat aacattacca cggctaacgt
    52501 gatacatttt aaaattttag acatgaatac tcccccttag acgataagat ttcctgtttt
    52561 ttcatcaaaa aagtgacact tattcatatc aaatgctagt gtaatcttgt cgttcgccat
    52621 cacttgagtt cttgaatcta atttagaaat caattccatt ccttggaatg tgctatgtac
    52681 cataatttct gaacctaaca gttcggatac aactacttca gattcaaatt gtgtctctgg
    52741 agaagtttga ataaatattg gttcttcatg aatgtcttca gctcgaatac ctaaaataat
    52801 ttccttgtct aagtagccag cagcttttaa cttttcaaat tttttattat gtaatttaaa
    52861 atggtgtgtt cctaccttca atccgtccat ttcaactgta gcattcaaca tattcattgc
    52921 tggtgagccg ataaattgcg ccacaaatat gcaattaggg gcatcatata tttctctagg
    52981 tgtgccgact tgcataatgt cgccatcttt caaaacaaca attcgactag ccatcgtcaa
    53041 tgcttcagtt tgatcatgtg taacataaat tgtcgtagta ttaagtcgct tatgtaattt
    53101 caatatttct gtgcgcattt gtactcgaag cttcgcatca agattcgata atggttcatc
    53161 cattaaaaag actttcgcat ccctaacaat agctctgccc aaagcaacac gttgacgctg
    53221 tccgccagat aacgcttttg gtttacgacc aagatactca gttaatccta atatttcagc
    53281 tgcttcatta actttttgtt caatctcttt tttatttact ttacgtagct ttagcccaaa
    53341 tgccatattt tcaaaaacag tcatatgtgg atataatgca tagttttgaa ataccatcgc
    53401 aatatctcta ttctttggtt caacatcgtt catgcgttcc ccatcaatat aaaaatcttc
    53461 agatgtgata gactctagtc cagcaaccat tcgtaatgtt gttgatttac cacatcccga
    53521 tggtccaaca aatacaatga attctttgtc agtaatatgt agattaaaat ctttcactac
    53581 agtattgttg ttatcatacg tctttttaat atgctctaac tttagttctg ccatgttatg
    53641 cctcccatac tttgtttaca gtttgattgt gcaatacact tcgtgaaaac gtttgcacaa
    53701 atacttacat aaatcatata ttcagtcatt attaatgtca atcataaatt ataaacgttt
    53761 acattactgt cagaattaaa cttttataca gaaaattgat atagtgcaca actattttct
    53821 agtgtgacaa taatgttttt tatgtgcgat atttcactgc caatgctaac aaccttgtct
    53881 tacattttaa gtaatatcta aatcaaacaa gtatgactta gcattacgat tttcattgaa
    53941 aaaagcaacc attatgtaag tatcatactc acacaatgat tgcttaattt tttagactgc
    54001 gggcaccctt ctataactac aaatcatata ggctacgttc cccatcaggt aactgatcga
    54061 taaacggttt tggatcttct gcatatgcat tacctacgcc acccttcatt ctttgaaaat
    54121 gtaaatgtgc gcctgtcgtt tgtatacctg tattgcctga atatgcaata atatcacctg
    54181 ctttgactcg atcacctact ttgacattat atttgtctaa gtgtagatac cactggtgat
    54241 attctccatt gtcttcggca atctgtaata ccttgccgcc caattcatta ttaaagatac
    54301 gtgttacttt accattcgtc ggcgctttaa ttggtgtacc ttttggcaat gcaaaatcaa
    54361 tgccataatg cttaccatca aatggtgaat gttgatattt accaaaagtc tcagtaatcc
    54421 gactccctct aaaaaagtct tcccattttg agtgtccttg tcctttatga ttttgtgtca
    54481 atttatcggt atattggtta aaccattttt gaatgtcatc tatataaata atcgtaaata
    54541 atagtgaaag aaaaatgata attgttgcca aaatacgttt tggtcgcttt gtcatagtgg
    54601 catccatcct tcatatattt tgggtattaa gtatatattc taaatcaact atttcataat
    54661 taaatgcact catactttag tagtactgcc aaatatagat gaagaaaatg atggtaattt
    54721 gaatattttg attgatatta aattttatat cgagtataat aattatactt aagaaaataa
    54781 cagaaacaga ggagaatttt caaatggcaa aagtattata tattacagca caccctttta
    54841 atgaactggt atcaaattct atggcggctg gtaaagcatt tatcgaaaca tatcaacaac
    54901 aacatcctga tgatgaagtg aaacatattg atttatttga aacttatatt ccagttattg
    54961 acaaagatgt attaactggt tggggaaaaa tgagtaatgg cgaaacgtta actgatgatg
    55021 aacaaatgaa agtttcaaga ttgagtgaca ttttagaaga atttttaagt gcagataaat
    55081 atgtatttgt aacgcctatg tggaaccttt ctttcccacc tgtagttaaa gcatatattg
    55141 atgctatttc aattgcaggt aaaactttta aatattctgc tgaaggacca caaggtttac
    55201 taactgacaa aaaagtatta cacattcaat cacgtggtgg atattatact gaaggacctg
    55261 ctgctgattt tgaaatgggt gaccgttatt taagaacaat tatgacattc ttaggtgtgc
    55321 catcatatga aacaatcatt attgaaggac acaatgctga acctcataag actgaagaaa
    55381 ttaaagcaac tagtattaat aatgctgaaa aattagcaac aactttttaa atttttagtt
    55441 acactgtgtg acgtctcaaa cgtcatgcag ttttttattg tatctctttt ccgcttttta
    55501 taaatatcta ttattttcat aaatttaaaa tttgatattc actttaatac tgatgtaaat
    55561 atttttcaaa atatattatt atagtgttaa tgaagtattt tgagaaacgg tgggaatgta
    55621 tgaaagcaca taaaattttc tggttaaact tagcagcaat tattattatt tcaattgtag
    55681 tcagtggcga tatgtttttg gcaatgaagt gggaacaaat tcatttgaaa gatggtctta
    55741 aaaaagtact aagtacgtat cctattaaaa acttagaaac gctttatgaa attgatggtc
    55801 acgataatcc ccattatgaa aataatgatc aagacacatg gtatatagaa tcctcttatt
    55861 cagttgttgg atcagatgag cttttaaaag aagaccgtat gttgttaaag gtagataaga
    55921 acacacataa aataactggt gaatatgaca caactacaaa cgataaaaaa aatgctactg
    55981 actcgacata caaaagttat ccagtaaaag tagttaataa taaaatcgtt ttcacaaaag
    56041 acgtaaaaga tcccgcgctg aaacaaaaaa tcgaaaacaa tcaatttttg attcaaagtg
    56101 gtgatttaac aagcatttta aatagcaatg atttgaaagt cacacatgat cctaccactg
    56161 attattataa tttatctggt aagttgtcga acgataatcc aaacgttaaa caattaaaac
    56221 gtagatataa tattcctaaa aacgcatcaa caaaggtgga attaaaggga atgagtgatt
    56281 taaaaggcaa taatcatcaa gatcagaaac tttattttta tttttcaagt cctggaaaag
    56341 accaaatcat ttataaagaa agccttactt ataataaaat aagtgaacat taatacttat
    56401 gctgtaatta tagaaacatc caaatcatct attaaaatcc tatattataa aaacacctca
    56461 cataactcgt tcaactgtac caaaccacat tacattagat tttaggctaa ctattgtgat
    56521 gtacatcaaa aacgaatttg tgaggcgttg tatattttac aaaggtgact agcgtttcgt
    56581 atagcatttc caacattact acactcaagc gtcacgctaa agttcgaaat cgaatccttt
    56641 cattcaacaa aagctcatat ccactacaaa cttcatatca agcgtataaa ctatcttgtg
    56701 atactatctc gatcatatct atagtatgca tttgtgttcc gtttcactga agtatatgta
    56761 tcatcagtta agtataaacc gtcatccttc aatgttactt gataagcata tttccgtgct
    56821 aaccaggcaa tatctatata attttctcct gcgttttcat aacttcttaa atcttcaata
    56881 tgtgcactaa cttcagggaa aatgattcta acaacacttt catcaaccca atatttgtca
    56941 tgcatccatc gcacttgatc tgccaataaa ggtaactgca catcattgaa atatagacga
    57001 aagccgtcac tatcatacat ttgccgatat ggtaatggct gttttctaat cactaacacc
    57061 tcgccaccca ttacggtgcc ttctctagta tcatcacttc cacccgaagc ttcatacgtt
    57121 gttgggtcaa cctgtagtcc atgtacatct ccaatataag catctggttt atgttccatt
    57181 gcatgtccat gtgcaatcaa tgctaatatt gtagattgtg aaaattgagg ctcccattca
    57241 atgcgattag gatggctact ataaattcta ggttcatcta tagcctgctg aatatccatg
    57301 ccaaacacta atacattgat taatgtttgc gcaacactag caatgatact tatggcacca
    57361 ggtgcaccta ctgttaatat tggcttcccg tgatacatca caatcgttgg agccatgtta
    57421 cttagtggtc gtttatatgg tgcaatttcg ttaataccac catctactac atcaaagcca
    57481 tccattgtcg tattcaataa cacaccgtag cctggaatcg tgatacctga accataaatc
    57541 ataccaattg atgtcgtaaa tgaagcaata ttaccttcct tatcaatcac acaaaaatga
    57601 gttgtttcgg tatgattttc ttcaacatct gtatgactaa tgcaatcaga cacaacacca
    57661 tgctctatat caatatttgc tttattgcta tcaatgagcg tactgcgtgc ttttaaataa
    57721 tcatcatcaa ttaatgactg tacaggcacc tcatgaaaat tatcatccgc caagtattgc
    57781 gcacgatcac tatatgctaa atgcatcgct tgtatcaaat gatgcaagta atcaacagat
    57841 cttggaccca tagatggtaa atcgacatgt tctaataact tcaatatttg aattaccgtg
    57901 ataccgccag aactagatgg tcccattgaa taaatgtcat agtctttaaa tgttgcactg
    57961 attggcgctt taatctgaat gtcatatttg gctagatcct ctaaagtgat tgtcccacca
    58021 catgctttga caacattgac taattgtttc gcaatgtcac ctttataaaa tgcattaaac
    58081 ccttgttctc ttaatatttg aaatgtctta cctaattcgg gttgtacaat ccaatcacct
    58141 tcacgccaat attgattttc atgcgtaaat acttgtgccg tttcatgata ctttgtcaat
    58201 cgtgcgtgtt gctggcgcga atatttttca gtagcccaat tggctgcatg accttcaatg
    58261 gctagttcaa ttgcaggatt aattaaatct tccaatgaca atttagcata acgcttgtga
    58321 atataatcaa acagctttgg aattgctggc acagcgacag ttttaccatg tgtagtcata
    58381 tcaaaaaatg atttatattc gcctgaatca tctagataaa attgtttgtc tacatgttca
    58441 ggtgctgtct cacgtgcatc aaacgcagtt atactgccag tactttgctc ataatatagc
    58501 aaatacccgc caccaccaat acctgatgca aatggttcta acacattcaa tgccagttga
    58561 attgcaatca ctgcatccat ggcgttgcca ccttgatcta atacatcctt accaatttta
    58621 gccgcaagag gatgtgatac ggaaattaac ccttctttag atgtttttgt ctgtttgtca
    58681 tttaagttaa tgaccatact atatcctcct actttctgtt aaatatttaa aacattattg
    58741 attaatggct ttttctactt tttctaaatc ttgacgttgc tcgttaccag tatcgacaag
    58801 tggtgtaatc ggtgatgcaa ttttaaattt atcgccacga taaaacttaa taaattgatc
    58861 ctgatctatc gcattaacta ctgcttgtct caagtttgga tgcgtcttaa atataccttt
    58921 tttaatattt agcattaaaa agactgactt gcgtccattt ttgcgaataa tgcttaaatt
    58981 tttatccgac ttaattaaat caaaatgttt ttgattcaca tctgccaaca tatcaattga
    59041 atgatttcta agttctgaca atgcattatt cgggtcacca ttaaacttca atgtaatatt
    59101 tttaatttta gctggtccat aactaccttt ttctgtttcg ttgaatcctg gattacgttg
    59161 aaacgttgct tgatatgcat ttttctgtgt cataatgtat gcgccacttg catacagcgc
    59221 atttttccca tctgaatttg caggaattgt actgctatcc ccatatcctt ttggatattc
    59281 ttgatttact tgattaacaa attttttaga taaaatgcct gccgaagagt gtgttaagta
    59341 atttacctct cgaggcatcg attgatctgt cgtaatttta acaatttgat aaataccgtc
    59401 tttattattt actttttgac catctgtcgt taacgatttg acgttataag ctttaatcaa
    59461 cttatcatag attgatttat cgtccttgtc tttctcttta cgcaactgat cgatgtcctc
    59521 atcttttaat atcttgatgt catttatatg tttgtgcata ttgtaagtat tattgttagg
    59581 cacagacttt ttatcacgtg ctctatctaa agaaaactta acatcttcag ccgatacacg
    59641 ctctccagta ttacgtgctt gtccattgac cactttcgca aaataatcat catctcttaa
    59701 caagaaataa aatgctttat tgtccttatt cacagcataa tcatgactta acgaaccttt
    59761 cgttgttaaa tgatcatttt catctaataa taataacctt gtgtacatat tcatattaat
    59821 tgaatatact gacggcgcaa ttgaacgtat tggatccaat gtaggaattt caccatcttg
    59881 ttgtgtcatc acaagtggcc gcgtatctcg ttctctacta ttgttgtaat caaattgttg
    59941 ccatattaat gcacgtgaat ttggcaatcc aacactattt ttatctaaca ctttattgtc
    60001 atatactaaa ttcttttttg atccatataa aggcgccata taccctttat caaatacaac
    60061 ttcatcttca atttgcttat atgtttgttt aacatctgct tcattttgag tagaagcttt
    60121 atttaacaac tggtctacat gtttatcttt caataaacta tttgatcctg tagaactaaa
    60181 taatgccgtc atagcatagt tcgggtcacc aaacactgtc atccagtcat caatttggat
    60241 atcataattg ccggcttgac gttgtgtacg atagctacca taatctggtt ggatattcat
    60301 cttcacgtta aatcctgcat tttccaattg atctttaacg atattcatat cattttcata
    60361 acttgcttgt cctaggaaat gtattgttgg tcgctcgcct ttcacttcaa ctttcgatga
    60421 cttttgagcc acttctgatt tcgtagggac accacaacca cttaatacca acgctaaaac
    60481 tataattgcg atactaatga ttttcttcac atctatccct acctttttaa tgaattcttg
    60541 gatctagtgc atcacgcact gcatcaccta taaaattaaa tgctaaaacg acgaacataa
    60601 tacaaacacc aggtacaata gctaaattac tgtgcgtttc caagtagtta ctaccggtac
    60661 gtaaaatgtt gccccattca gctacatcag gtgcaacacc aagtcctagg aaacttaaac
    60721 tacttgttgt taatacaacc acacctatat ttaatgaaaa acgtacaatc ataggcgcaa
    60781 tcgcattcgg taaaatataa cgccatatga tattccaagt gttttcacca gtgatacgtg
    60841 ctgcatctac atattccatg cgtttaattt ctaaaacact ggcacgcatt gtccgtgcaa
    60901 atgatggtat attaccgata cttaaagcaa taattaaatt tggaatactt gctccaaatg
    60961 atgcaataat tgccaccgct aacaataatg atggaattgc aaacactaca tctaaaattc
    61021 gcattattaa attatcaata tgattaaaat aacctgcgat agtgcctagt aacacaccaa
    61081 aaataactgc aataactact gaaataattg aaattgaaaa tgtcagcttc gttcctacaa
    61141 ctacgcgtgt aaataagtct ctaccgaaat catcagtacc aaacggatag gctagactcg
    61201 gtccatgtaa cagtgcattg aactgatttt tagtagccaa tgtcgtatca aatgtaaatt
    61261 gtgacacaat tgataatgtc agcatgtaga ctaaaataag taacccgata atcgcaatac
    61321 gatgtctagt agtttttcgt ataaacgatt cccacccgtt ataactatgt atttgcgatg
    61381 tacgttggta acgtctaata cttacaaaca ttaataatgt aaatacgttg cctgttaatg
    61441 tcatcaacaa taacaacact tcgacgatac gtcgccatag gtcatgatgc ttccatgttt
    61501 gttccgttgt taaaataata attaaaatga tggttaaaac gattagcaat gtttcagcaa
    61561 tatagaacgt atcggccaca taacctttaa aaagatttaa tgcactcgtt aatataacta
    61621 aaatataagt tgctatggcg taacttgcga ataattttaa ggaagctatc tttgaattaa
    61681 gttgtgccat atgcctcact tcctttcgtt gatttcacta cgtaattttg gatcgattaa
    61741 agcataaaat atatcaataa ttaagtttgc taaagatatt acaattgata tatatacgac
    61801 cccacccatg actgctggaa tatcaggtat tagttgtttt tggacgatat aacgcccgat
    61861 accattaatg ttaaatactt gttccgtcac tgctgaaccg cctagtaact ctgccactag
    61921 aagaccaact aacgttacaa ttggaataat ggcatttttc aaaatatgtt taataacaac
    61981 ttgtgtcgtc gataatcctt ttgcataagc agttaaaaca taatcgctgc gcattacttc
    62041 aagtacagaa gaccttgtca tacgcgtgat agaagcagca atacttgttc caatgacaag
    62101 tacaggtaaa atcaacgata ttggatgttc tggcatataa gatggtggca aaatatccaa
    62161 tttcaatgag aacgctaaaa tgaataatag cccttgccag aaacttggaa tagataaacc
    62221 aattaatgca attatcatta acgtgatatc aagccaacta tttcgcttca tcgcactgat
    62281 aataccaatt ggtattgcaa taattaatgc caccattagc gctaatactg cgacaattat
    62341 tgtaattgga attctttcgc caactgcttt agtcacaacc tcattccctt tgtaagtcgt
    62401 acctaagtca aaggtaaaaa cacccttgat ggtatcccac aattgaataa aataaggttc
    62461 gttaagatga tgtaatacat tgaattgatg tatctgtgcc tttgttgcat tttgtcccag
    62521 tatgctataa gccgcatcaa gcggtgaaaa atacagaatg gtaaacacac tgacaataac
    62581 accaatgatg acaatcacag ccatgacaat tcgttcaaaa atatatctaa ctaatggctg
    62641 taaataaaaa gtcaataaga tgaacatcgg caaggccaat atcactttga tcatgatgaa
    62701 cttatgaaat aatacatttt caaagtatgt tgaaaaatgt gcttgttcaa tattctttga
    62761 actcgtatta gaactttgtg ccttgaatat ttttaatgct tctttatgta tttgtgtgga
    62821 tgacttttgc tgcgataaat atttatattt ttgatgtaac gcctgttcaa tttctgaaat
    62881 ttcagaatta ttagcgtaaa aatttttcct cttagcagaa aagaaaaact ttatcactgc
    62941 atataaaaat attggcaagc ttaataccga taatacaaac ttgtaaaatg ccttgtgata
    63001 caacgactta aaagattcta aataagtaat tatgataatc actccttgta aaataatctt
    63061 gagtagatta ctatgatata caaaagtata gaataaattt acacatttgt gaatagggag
    63121 gcacaacatc atgtcaaatt tattagaagt caacagtctg aatgtacaat tcaattatga
    63181 tgaaactaca gttcaagcgg taaaaaacgt ctctttcgaa ttacgaaaaa aacatatcct
    63241 aggtattgtt ggtgaatcag gatcaggaaa aagtattacc gctaaatcta ttttagggct
    63301 actaccagat tatccagatc acacattaac aggagaaatt atttttaatg ggcaatcgtt
    63361 aaataattta tcaacttcag cgttacaaca aattcgaggt aaggatattt caatgatttt
    63421 tcaagatcca ctctcttcgt tgaatccaag attaacgatt ggcaaacaaa ttacagaagt
    63481 aatatttcaa cataaacgtg tatctaaatc tgaagcaaag tcgatgacaa tagacatttt
    63541 agaaaaagta ggtataaaac atgcaactcg acaatttgat gcttatccac atgaactttc
    63601 tggtggtatg cgtcaacgtg tcatgatagc aatggcattg attttaaagc cacaaatttt
    63661 aatcgcagat gaaccaacaa cggcattaga tgccagtaca caaaatcaat tactgcagtt
    63721 aatgaagtcc ctttatgagt acacagaaac atctattatt tttatcactc acgatttagg
    63781 cgctgtgtat caattttgcg acgatgtgat tgtaatgaaa gatggaagtg tcgttgaaag
    63841 tggcacggtt gaaagtattt ttaaatcgcc acaacatacc tatacaaaac gcttaataga
    63901 tgcgattcct gatattcatc aaacgcgtcc gccaagaccg ttaaacaatg atattttatt
    63961 aaaattcgat cgcgtgagcg tggattacac atcaccgagt ggcagcctat accgagcagt
    64021 taatgatatt aacttggcta ttagaaaagg cgaaacatta ggcattgtcg gtgaatcagg
    64081 gtcagggaaa tcgacattag ctaagacggt cgtcggtcta aaggaagtgt cagaaggctt
    64141 tatttggtat aacgaattac cattaagttt atttaaagat gatgaattga aatctttacg
    64201 acaagagata caaatgattt ttcaagatcc attcgcatct attaatccaa gatttaaagt
    64261 cattgatgtg attaaacgac cactaatcat tcatgggaaa gtcaaagata atgatgacat
    64321 tattaaaact gtcgtatcgt tgttagaaaa ggttggccta gatcaaactt tcttatatcg
    64381 ctatccacac gaattatctg gtgggcaacg tcagcgtgta agtatcgcga gagcacttgc
    64441 tgttgaacct aaagtgattg tttgcgacga ggcagtgtcc gctttagacg tttcaattca
    64501 aaaagatatc atcgagttat taaaacaatt acagttagac ttcggcatca cttatttatt
    64561 catcacacat gacatgggtg ttatcaatga aatatgtgat cgcgttgcag ttatgaaaaa
    64621 tggcgaaatc gttgaactga ataacacaga agatattatc aaacatccgc agtcagacta
    64681 tgcaaagcaa cttatttcag aagtagcagt tattgctaaa taaaagtcat gcgttgtgca
    64741 actttatcac tgtatggtct gaaataaatt gcgcgacttc tgatgtgtta tgttagatga
    64801 ctttgtcata ctaactatat tgtgctaagt tagggctcgg caccatatca agttttaggt
    64861 gctttgccat gatttaagag tcacccccat actttgggca ttttaacgcc agaataaatc
    64921 ccccgccact atgtgaagtg cgggggatta tttatatttt attagaatat tcagattttt
    64981 gagtgtgtca acttagctta gtcaatgtat atttaacgtc acttactctt tttctttcat
    65041 aattaacaca ttcaaataaa ctttgatcaa aaaacacaaa gttaaaagta ccatcttgta
    65101 atatgctctc atacattatc ccgtcatatt taaggcttcg aatataatca gctaaatatt
    65161 gaaatggcaa ataatctatt ccttgttcat cgcttggatt tgttattcct ttatgaatct
    65221 tttttaatgt ttggtaattt acaaaatact ttctaaatcc atcatcgcca gctttgattg
    65281 cattactagt taaattagtt aaattcgcaa ttttcaattt ctcttttgtc acgttttttt
    65341 gtaacttaac cttacctata taaataatgt cattatgctt aggtttaact tcttctatac
    65401 tgacctgttc ttttgtacta aggtataata cgcttatcca tttagaattc aatcttcctg
    65461 ccgttgcaaa tccctttggt ggtgacatta gttcactttt ctctgtaatg aacttaacta
    65521 ttctagatct atataatggt tcaaatcttt ctctaaattc ctcaatacta tagtaattag
    65581 tagtgatatc gagaaagaac gctaaattct ctaaattgat catattatta tgaaatctat
    65641 ttttatactt caagctctca caaaatccat cccagtcatt atttgctaca attagatttt
    65701 tatttgtata ttttttatcg tttatgattt tagcgcctac taaatcttcc aacactcgtc
    65761 tatctaaatt ttcatcatct ttaaaaagtt catttaaaat acaacttatt tgagcttcct
    65821 caacattaaa tatactccag tcgtctttta atgctatttc aatcttttta ccttcttttg
    65881 ggctaaaagt atctggtaaa tttatactaa tatcatataa ttctaatgct ggtcttaaat
    65941 aatctctaat aagttctaat ttatctatgt ccttagtcgt atcaaatatt ttaacaccaa
    66001 gatgattgtt atcaatatca caattgtcaa atttgctatt atcatttgca atgatttcta
    66061 cgatttcagt attattaaaa catttttcac atattttcat tttgagactc caagtatcta
    66121 ttcataattt ctaggtgatg catgatagat aaccttttaa ttaaacctaa tcctggatac
    66181 ttattatttt catttaattc ttcaaattgt ccaagcgcat aagatctatt tttaatatct
    66241 aagttttttg accatgttac taatttatct acagcctcaa aaaacttttt acctggattt
    66301 gatctatcat tattagaatc agagacaaaa tgcttaactc ttagctcatt agcctcatca
    66361 aaatagacaa tatgtatcgc aatagctaat ggcgaaaagc caccgtctac atattctcca
    66421 cctataacag aatagtctga aaatgctacg tatccatctt ccttataata aaggtgatcg
    66481 tcgctataaa actcgtcatc attatctaaa taatcaacat tacgacttag tttattaaaa
    66541 ggatccataa tgatgccttt gtttggaata ttacgcaatt tacgtttaat gtgtctatca
    66601 tctggtacaa aaaccatttc aagtttattt ttatctccaa acatttccca atcatctaca
    66661 cttgttaaaa tagctaaact attacttgaa aattgctgtt tcaacctatc attgccttca
    66721 tcagaaggac ccaagtaagc tttttgaata acacttttgt tttttgttat tatttcagtt
    66781 aaatgtgcaa cagtttcgtt actaatctct tcttcagtta actttgaatt tactactaaa
    66841 tttatagaga atgctttttc accacagtat tgcaaagtat ttttaaatgt ggttgtatat
    66901 ttaattggtt caattatagg ttgaatacaa tcaccaatca aacctttctc taacaattct
    66961 ctaattgcaa atagttcatt ttgtttccca cgcaaataag gataatacat attaatcctc
    67021 cttaagaaaa tgatatagtt tatttttatc tttctttgtc atttttgcga aatataacaa
    67081 gaacttaatt tcagccggcg ctgctaaaaa ttcatctttg tattctttca tgtttcctct
    67141 ttttttcaac tcagaaatca tattgttatg cagtttcgtc attggtattt ctttaaacac
    67201 gtcaaataac acatcatact ctgtgaaatc attataagtt ggcacatcca tatgattttg
    67261 tgcaataact ctttttcttt cttcttttcg taaaaagttg tacatcgctt ttgtgtttaa
    67321 gagactattg ttttctttag gtttttgaac ttcactcagt gtatttctag ttgttaacac
    67381 taaaattcca actgttgtat ctttgtatct agccataact ttattcagat gtttgtcatt
    67441 tgtaataact acgacataat taaacacttc ataataatca ttaatttgat tatctaatct
    67501 atccagctta tctaattctg ttttaatctc atagacaacg cctttgccgt ttaacaatat
    67561 aaaatcagca atacttttcc ctatgggcat ctcagaaagt gcagtagttg tattaataga
    67621 atgtcgtcct agaaggagtt tattaagtat tgtgtttttg taaaaatatt cgttacgata
    67681 agacttttta agatagttat ataaagattt catcttctgt attacattga tatttttagg
    67741 tccctgtata taaaaatcat atgcctcatc aacaacatca cttttatttt tgattgcaat
    67801 ttgatgtaat atcttttgac taaaaacacg attaatcata tttctattat ccatagctac
    67861 gcacctcctt caacatacat ttaatactta tcctgttaat attataaact attttaaatg
    67921 cctatagaca ttatttctat acacatacga ttataaaatt gtattgaaac caaatgatgg
    67981 ttgccaaaca catggtaact aacaaaagat agaaaataat aatgccccct acactaatct
    68041 agcgaggggg ctgaatcatt acacaccgta tttttcagtt gtttctgcca cgaaagattc
    68101 aatggctttt cttgctttac gcttttcttt caatggcaaa tcaccaatca tttttttaag
    68161 gtgatgtggg tttacaacac cactatactg gtagtcattt gaaattgttt ttagggcttg
    68221 ttcatcgata gatctctctc cagcaaatcc tttgaactcc gcttcttttt taatactttc
    68281 gaaattaaca tattcttgat cgatatcatc atccttattt aaagaaggta caacattgtc
    68341 gatgaattct ctaattagat ctcgttttaa cctcaatgtc ggatcatctg catgatctaa
    68401 aatgcgtcta atttgttctt ggttacgacg ttgttccgct ttgtcttcaa gatcaatttg
    68461 tctcaatata ttcataatat aattcacatt aatcgtatca ttacgcatca tttctatttc
    68521 gaaatcaata tcatttaaaa tggatacttt atttttctca gccgtcgctc tttttacttg
    68581 atcgtacaca gctaaatatt tacttttata gtcttcattc tcttgttcat ccattccaat
    68641 ttcatcaatt gtaaactcaa actcgtcaaa tgcttttaaa cgtaatatta ttttagctaa
    68701 taaacgataa gcttcaacaa agcgctttag ctcttcttca tcttgaatgt catcaaccat
    68761 gtgtggtgtc ggcacaatca ttttaagctc acgataagcg tccataaatt cttttttata
    68821 ctcttcataa ctgcgcatta aaattgtatc cgtatcattt gtttgtgaga atactctcag
    68881 tgcatcgtct gtctcttttt tcaagtcacg atagtttaca attttaccaa atggctttga
    68941 ttctttttca accctatttg tacgtgaata cgcttgaatt aaatcatgat acattaaatt
    69001 cttatcaaca tataaagtgt tcagtacttt actatcaaaa ccagttaaga acatattaac
    69061 aacgattaag atatcaattt tactatcttt aacgcccttt ttaacgtttt ttgaaatatg
    69121 attaaaatac tcattagttg tgtctgttga aaaattcgtc tcgaactttt tattataatc
    69181 actaatcatt atctctaatt tttcacgtga atgatatggc acttcaccat cacgatcatc
    69241 ttcattaggt ttaaacgtaa atataccagc tatcgttaac ggttgttcca actttttgtt
    69301 aagtcgctta aatgtctcat aatatttaat aagcgcgtga atactttgga ctgtaaatat
    69361 acttgaatat tgacgattac gtgtatattt atcatgatta ttgatgatat gtcgtgttac
    69421 taattccaca cgtttatccg ctaaccatac ttcttccgta tcaattgctt caaccatgct
    69481 gttatcttct gcttttaaag ctttattttt aaaagtatta atatagtcaa ctgagaaacc
    69541 aagtacatta ccatcatgaa tggcatctct aattaaatac gtatgtaagc atctaccgaa
    69601 aatatctgca gttgttctac catcttgact actattttct ggaaaacgtg gcgtaccagt
    69661 gaatccaaag tattgggcat ttttgaaatg ttgtttaact agacgatgca tgtcaccaaa
    69721 ttgactgcga tgacactcat caataataaa tacaacttta ttcgttttat actgttctaa
    69781 taaaggggca ttcccttgaa tcgctttagc cattttttga atcgtcgtta caataagtgg
    69841 caaactttta tcatttagtt ggcgtaccag ttgcgaggta ttaaaagttt tgtctacagc
    69901 acccttagca aatttattaa attcctcttc tgtttgacta tccaagtctt tacggtcaac
    69961 caaaaagata actttcttaa tgtcatcttg ctgtgataaa atctgactcg ctttaaaaga
    70021 agtcaacgtc ttaccacttc cagttgtatg ccatacatat ccattattcc ctgtctcagt
    70081 cgcttgttga ataagtgctt ctaccgcata cacttgatac ggacgcattg ccatcagtat
    70141 tctatctgtt tcattaataa tcatatagcg cgatatcatc ttagctaatt gacaaggtct
    70201 cataaatgac tcagcaaacg attgcaatgt attgatacgg ttattctgtt tatcactcca
    70261 ataaaacatg tgactcttca atagttcgct atcattatta gaaaagtatc gcgtttcaac
    70321 accattacta atgataaaca tttgtatgta gcggaataag cctgtgtaat tttgtttgcg
    70381 gtaacgtttt acttggttaa acgcctcatt aatatcaata cctcgacgtt tcaattcaac
    70441 ttggacaagg ggtagtccgt tgattaatat cgttacatca taacgtgctt tatatgtatc
    70501 ctcgacagat acttgattcg tcacttgaaa cttattttta caccaacttt tcgtatctaa
    70561 aaacgacaaa taaatctcag actcatcatc acgtctaagt ggtaatttat cacgtaaaat
    70621 acgggcactc tcgaaaatac tttttccatc aatcatcgtt aacagacgtt gaaattcttt
    70681 atctgttaag ggattgcctt ctaatttgtc cgcatgacgc tcatttaaaa tcgttctaaa
    70741 attatcaagc aattgcttat tatcacgtat cgttactctt tcgtaaccca attgttcaag
    70801 ttgattcatc atttcatttt ctaatgcgta ttcactttgg tatgccattc atatcccctt
    70861 ccatacactt tctattgctc taaatatatc ataaacttta atgaaaaatg tttgtttttt
    70921 atcttcaaac gtaaatttat tctaatttta ttgtcttatc ttttaatatt tgtctttgag
    70981 gtaagtcgta tactaaaatt tgaatacaaa taatcaaatc attgataaat tttttgtcta
    71041 cgattaatgg agggacttga atggtgttaa ttacctatca aatcatttta ttttttatta
    71101 ttagtctaag ttactattta actttaaatc attacatggc agtcactgta ggtaacttca
    71161 cttcaatatt cggcatgttc gcagccatac tctttatgta ctactaccta ctctataaaa
    71221 gtcccgaata caatcaacgc aaacgattta aacatttcat tcatatcact aatttgataa
    71281 taattgcttt tagcaccttc gtattagttc atttagcatt aaaattattc ttcagcattt
    71341 aatttccatc tatgaaaaaa gcaaagctca aatctgaact ttgctttaat ttgtcacgcc
    71401 tttatcattt tcaaaatagc ctctatgcca gttttacaaa cttgtagcaa caatttttca
    71461 tcaagcaact gaatcacatc aaaaacttca attgtttacg ttgcaactaa ctttatgtta
    71521 gaacgcacac aatatctaat gtttaaaatc tatcgtcgca agatgcttcc tgtaattatc
    71581 aagtgccatt ttcgattggg ttatacaatc tagaatcgca tgataattta atgctacaaa
    71641 tcgatagtac aatatatcta ccgtgaataa ctgtgcaaat agtgacgttg tagccgccat
    71701 acgcatttca ttttcatcag ttctgccata aatcaatgca tagtctgcaa tttgagccac
    71761 tggattatta gctgtactag atatagttat gatgggaata ctgtaatgtg tggccacctg
    71821 tgcaattgac tgcaattcac tatgactacc ttgattcgtc acaaaaatca tgcaatctct
    71881 atcatcatgc gtcgcaaatg ttgacacaag taaatgcgtt tcatgtaata acctgacatt
    71941 taagccaata cgagataact tttgaaaaag atcaccaata gtcaaactcg atgcgccaaa
    72001 tccaaataaa aatattgtcc tggcattttt caacacatca caaattgcat caatttgcgc
    72061 atccataata ttagtagcta caaatcgcat cgtattcgtt gctctagcaa tcattttatt
    72121 tttcaaagtt tctacagatt cattttcaat caattctaaa tgtggattgg ttgcaatatc
    72181 ttcgggtaag tatcgagata tcgcaatctt tagctcttga aaaccttgat gtgtcatttt
    72241 ccgactaaat ctaacaattg atgctgtact aacattcgta acatctgcca aatcattcac
    72301 agtcatatca atgattttat gtggattctt taaaatgtaa tcagcgatta tcttttctgt
    72361 cttcgtaaaa tcactcaact gcttatcaat gcgatataaa atatttgtca tcattaatca
    72421 cccaacaaat ctgtctgtcg catcgccttt gtcgttccaa ataaatatgt acaaacgaat
    72481 ccaccagcat acgcagcaag taatcctgca atataaccta aatacatatt atctgagatt
    72541 aatggtaata gtgacacacc acttgggcct attgctttgg caccaatatg tccaattcca
    72601 cctattacag cgccaccaat accaccacca atacaagcag ttaagaaagg tcgacctaat
    72661 ggcaaagtca caccatagat taatggttct ccgataccta ggaaaccaac tggcaatgca
    72721 ccttttaaag tattacgtaa tgttgtgttg cgtttacatc ttacccaaag tgctaatgcg
    72781 gcacctactt gtccagcacc agccatcgct gcaattggca ataagtaagt agcacctgat
    72841 tggttaatca tttctatatg aattggcgta aaaatatgat gaagccctaa cataactaac
    72901 ggtaggaagc ttgcaccaat gataaatcca ctaaatacgc caccaatact aataattccg
    72961 ttaactactg aaactaaact gtctgaaaca aaacctgcta atggcataaa gataaagata
    73021 gttaatagtc ctacaatcaa caatgcaata gtcggcgtta caataatatc aatcgcattt
    73081 ggcacaattt tatgtaatct cttttcgaca atacttaaaa tccaaacggc aaaaataacg
    73141 ccaataatcc caccttgtcc aggttgcaat ggttctccag tgaagacatt cattaaaata
    73201 tttttaccag caatacccgt taataacgtt gtaccaccaa tcacgccacc aagtcctggt
    73261 gtcgcaccaa attctttagc cgcattaata ccagtgaaaa tagctaagta tgctaacata
    73321 ccgtctttaa tgacattaaa tactgttata agttgcgtaa tccaagcacc tgaaatatag
    73381 cctgccacca ttaagttact cagtactgct gcaataccac caattaatcc agctccaata
    73441 aatgcaggaa tcaacggtat aaagatattg gcaattgatt tcaatacttt attcaactta
    73501 ccattctttt gttttgcttt atgcgcttcc ttattcgcct ttgctttatc agctgcatat
    73561 gatttatagt ccattttttc actatcattg tgatggtgtg gtattgggtc acctagttta
    73621 acaccactta attccgccat atgattagcc actttattga ctgtaccagg tccaaccaca
    73681 acttgaatgc gttcatcgtg tataacaccc atgacaccat caatatgcct tagttcttgg
    73741 tcatctactt tattctcatc taatacttta atacgcacac gtgtcataca gttcatgaca
    73801 ctatctatat tatccatacc acctactgca gcaataattc gttctgcaag ttgttgttct
    73861 ttggtcattt aaatccctcc taaggttgtc tatctctgat tgctcgttta acaatgtcac
    73921 cattgtttaa taaccgtctt gttgcttctt ccttagaaat gccacacata cccataactg
    73981 tcgcaacttt cacatcatgc tcagatacct gatataacgc cattgcttca tcatatgtga
    74041 tagcacatat ttcttgaata atacgcactg aacggtcgat cagtttttga ttggttgctt
    74101 taacatcaat catgaggtta tcgtaaactt ttccgacacc aaccattgtg atggttgaaa
    74161 tcatatttaa aattaacttt tgtgctgtac cagactttaa acgtgttgaa ccagttaata
    74221 cttctggacc aactttaact tctactggat actgcgcaat ttcacttata actgcatgtt
    74281 cattgcatga aatagatact gttgtagcac cgattgtgtt agcaaatgtt aaaccgccta
    74341 taacatatgg cgttttgcca ctcgcggcaa ttcctataac gacatctttt gatgttaaat
    74401 ctatattttt caaatcttct tccgctaatt ttttgtgatc ttccgcacct tctacagcca
    74461 tcgtcatagc atgttgtcca ccagcaataa tacctataat ttcatgaggg tcagtattga
    74521 atgtaggtac acactccgct gcatctaaga cacccaacct tccacttgta cctgcaccga
    74581 tataaatcaa tcgtccaccc tttttatact gtgcaattgt ttttttaatt acttttgtca
    74641 attgtggtat tgcctttcga actgctaacg ggacttgctg atcttcttta ttcatcgtaa
    74701 ttaaagcctc ttccacagtc atttcatcaa gatgcatcgt cgcttcatta cgcgcttcgg
    74761 tcgtactatt ttccatcact tcttacactc cctagttttt tgaaaatcaa atgtatcatt
    74821 cggctcgata caacttaaca gtggtaagtc ttctttaata atttgtgcaa caacattcac
    74881 attgtcatgt gcactaagcg tttgtctcac aatttgcatt tcgccttgat aacgtccgtt
    74941 attcaaatta tcaacggtta ctgaaccaat gcgtcgttgc gtcgtaaact gtggttgaat
    75001 cgaatgtgga catatttgtc ttgacgtttc cgaacgaatg acattttccg gattatccgg
    75061 gcgtacttta tgacacatat cgaaaaggta agtcactgtc gtatcaaaca cttcttcaat
    75121 acataacgtg aaatgcctat gcttgcaaaa atctataagt tgttttgcct gcctcatttc
    75181 aatcaatgag tctccaacta acacttcaga tacaccagtt tcttgtaata atttagctgc
    75241 aacgacagga tgactatgtc tcgttgcttc aattgttggc aagcctttat gcaaaggacc
    75301 tcgcaaatca ctccctacaa taaaaccata tatttgtgcc tttggattaa attgataaat
    75361 gagttcattt ttcttattga ccaagtcaac agataatccc gtatctggtc ttggataata
    75421 gttatgacaa aatgaaagta atgtaaaatc attcaattgt tgatgtaagc ttgttaacaa
    75481 ttcccgggaa ataatacttg cattcaaaca gcactttaaa ccctgtgcca ttatcgcttc
    75541 gattgcctca attgatgtac tatgatcgat acgaatcata aattgtgcat catattgtcg
    75601 aagatggtca taaaaagatg gtgttaaaat agatggatta gcatctatga ggtaagtcac
    75661 ttgttcatgt tttaataaat tgagtagttt tgtgaaataa tgatattttg tctcgtcatc
    75721 ttcttctggt atttgtacag atgtaaaaat catttggtaa ccttgtttaa tcattcgctt
    75781 aatatacgct tcatctaaag gttgtcctaa atacactgaa aagcctgtca aagtagccct
    75841 ccttaacaat ataattatta ggaaaatata gttgagttgt gtaatcgctt acattttact
    75901 ataagagaaa acacattaca atattaatca gttaaagcct gttcattgta ataatcttac
    75961 atatttctgt cacaagttaa ttattacacc atcaaagatt atcctttctt ttaagtgctg
    76021 ataatagctg ctactgctgg attattacaa taacttttat acattttatt caggattatc
    76081 ttatattatg ttttaataat aatctgtgaa caattaagag atttgaaatt gaatttaata
    76141 attgtattga aaacgcatac ttcaccatgc taaaatagga gtcgcaaaca aataagattc
    76201 aataagatgt gatggtaagc tagataaact aacgtatcag ttttatatac tacttagtca
    76261 attgcttctt ttacattaat tacatacacc aacgtgttac taagtaagat taggcatgag
    76321 ttaaattaag ctgtgatggt taccaacaca gtctatttgc tcgtgtcttt ttttattgaa
    76381 tcttaaataa taaatacaac tttggaggtt ggacaagtga ggaagaaact tttcggtcaa
    76441 ttgcaacgta ttggtaaagc gctaatgtta cctgttgcga ttttaccagc agctggtctg
    76501 ttattagcta tcggtacagc tatgcaaggt gaatcattac aacactactt gccgtttata
    76561 caaaatggtg gcgtacaaac tgtcgctaaa ttaatgacag gtgctggtgg tatcattttt
    76621 gataacttgc ctatgatttt cgcattaggt gtcgcaatcg gattagctgg cggtgatggc
    76681 gtagcagcta tcgcagcatt cgtcggttac ataatcatga acaaaacaat gggcgacttt
    76741 ttacaagtta cacctaagaa tattggtgat ccagcgagtg gttacgctag cattttaggt
    76801 atcccaacat tacaaacagg tgtgttcggc ggtattataa tcggggccct ggcagcttgg
    76861 tgttataaca agttctataa cattaactta ccatcttatt taggtttctt cgctggtaag
    76921 cgtttcgtac ctattatgat ggctacaaca tcatttattt tagcattccc aatggcatta
    76981 atttggccaa cgattcaatc aggattaaat gcattcagta caggattatt agattcaaat
    77041 actggtgttg ccgtattctt atttggtttc atcaagcgtt tattaattcc attcggtcta
    77101 catcacattt tccacgcacc gttctggttc gagtttggtt catggaaaaa tgcagctggt
    77161 gaaattattc acggtgacca acgtatcttt atcgaacaaa ttcgtgaagg cgcacatttg
    77221 acagctggta aattcatgca aggtgaattc cctgttatga tgttcggttt acctgcagca
    77281 gctttagcaa tttatcacac agctaaacct gaaaataaga aagtagtagc aggtttaatg
    77341 ggttctgctg ctttaacatc attcttaact ggtattacag aaccattaga attctcattc
    77401 ttatttgtag caccattatt attctttatt cacgcagtac ttgatggttt atcattctta
    77461 acattgtact tattagatct tcatctaggt tatacattct caggtggttt catcgactac
    77521 ttcttactcg gtatactacc taataagaca caatggtggt tagtcattcc tgtaggtctt
    77581 gtatacgcag ttatttacta cttcgtattc cgattcttaa ttgtaaaatt aaaatacaaa
    77641 acaccaggtc gtgaagataa acaatcacaa gcggctactg cttcagcaac tgaattacca
    77701 tatgcagtat tagaagctat gggtggcaaa gcaaacatta aacatttaga cgcttgtatc
    77761 acacgtctac gtgttgaagt taacgacaaa tctaaagttg atgttcctgg tttgaaagat
    77821 ttaggcgcat ctggtgtatt agaagtcggc aataatatgc aagcaatttt tggtcctaaa
    77881 tctgaccaaa tcaaacatga aatgcaacag attatgaatg gtcaagtagt agaaaatcct
    77941 actactatgg aagacgataa agacgaaact gttgttgttg cagaagataa atctgcaaca
    78001 agcgaattga gccatatcgt gcatgcacca ttaactggtg aagtaacacc attatcagaa
    78061 gtgcctgatc aagtgttcag cgaaaaaatg atgggtgacg gtatcgctat caaaccttca
    78121 caaggtgaag ttcgtgcacc attcaacggt aaagtacaaa tgattttccc aacaaaacat
    78181 gcaattggtc ttgtatcaga tagtggttta gaactattaa tccacatcgg tttagacact
    78241 gttaaattaa acggagaagg ctttacttta catgttgagg aaggtcaaga agttaaacaa
    78301 ggtgatttat taatcaactt tgatttagac tacatccgca atcatgcaaa gagtgatatt
    78361 acgcctatta tcgtgacaca aggaaacatt acaaaccttg attttaaaca aggtgaacat
    78421 ggcaacattt catttggcga tcaattattt gaagctaaat aatgcttact ataaacaggt
    78481 gcgtatacct tcataaggtg acgcgcctgt tttttctttg ctattgtatt ttgcagcatc
    78541 attgatagtt cgctctcccc ttaaattttg aattttaaga tcatcaatta aagcccccct
    78601 tcatactcat ttcctaaaaa atattaattg ttcactattg ttagcgtttt cacaacaaag
    78661 tcaacttcct tgaccttaca ctatattcga ggctatcatt ttaagtgtaa atatagagaa
    78721 aaggtggctt tttttatgaa acaacgcatt ggagcttact taattgacgc tattcatcga
    78781 gcaggcgtcg ataaaatttt tggtgttcct ggtgatttta atctcgcttt tctagacgat
    78841 attatcagca atcccaatgt agattgggta ggaaatacaa atgaattaaa cgcaagttac
    78901 gcagcggacg gttatgcccg tcttaatgga ctcgctgcat tagttactac atttggtgtt
    78961 ggcgaattaa gtgccgtcaa cggtatcgca ggttcatatg ctgaacgcat acctgtcatt
    79021 gcgattacag gtgcgccgac acgtgctgtt gaacaaggcg gtaaatatgt acatcactca
    79081 cttggtgaag gtacatttga cgactatcga aaaatgtttg cacatataac cgttgcacaa
    79141 ggttatatca cacctgaaaa tgcaacaacc gaaataccac gtttaattaa tacagcaatc
    79201 gccgaaagac gcccagttca tttacattta ccaatcgatg tcgcaatctc tgaaattgag
    79261 ataccgacac catttgaagt gacggcaact aaatatacgg atgcatcaac atatatagag
    79321 ttattagcaa ctaaactgca tcaagcgaag cagcctatca tcattactgg acatgaaatt
    79381 aacagttttc acctccatca agaattagaa gattttgtaa atcaaacaca gataccagta
    79441 gcacaacttt cattaggaaa aggtgctttt aatgaggaaa atccatatta tatgggtatt
    79501 tacgatggga aaattgccga agataaaata cgagattatg tggacaacag cgatttaatt
    79561 ttaaatattg gagccaaatt aacagattca gcaacagcag gtttttcata ccaattcaat
    79621 atcgatgatg tcgttatgtt aaatcatcac aatatcaaaa ttgacgatgt tacaaatgat
    79681 gaaatatctc taccatcatt gttaaaacag ttatccaata tttcatatac gaataacgca
    79741 acgttccctg cgtatcatcg tccaacatca cccgattata ctgttggcac agaaccatta
    79801 acacaacaaa cttattttaa aatgatgcaa aatttcttaa aaccaaatga tgtcatcatt
    79861 gctgatcaag gtacatcatt ctttggtgct tatgatttag cattatacaa aaacaatact
    79921 tttatagggc aaccgttatg gggttctatc ggctatacat tacctgcaac attaggttca
    79981 caattagcag acaaagatcg tcgtaactta ttattaattg gtgatggctc attgcaacta
    80041 actgttcaag ctatttcaac tatgattaga cagcatatta aaccggtatt atttgtgatt
    80101 aataatgacg gctatacggt agaacgactt attcacggca tgtatgaacc ttataatgaa
    80161 attcacatgt gggattataa agctttacca gctgtatttg gtggtaaaaa tgttgaaatt
    80221 catgacgttg aatcatcaaa agatttacaa gacacgttta atgcaattaa tggtcatccc
    80281 gatgtgatgc attttgtcga agtcaaaatg gctgtcgaag acgcaccgaa gaaactcatc
    80341 gatatcgcta aagctttttc acaacaaaat aaataatttc atcgtataca gggtataagt
    80401 ttaagcgaat actttattaa acgaatagga ctctgatata agatgattaa ttttaataaa
    80461 accgctttag tgttaatcga cctgcaagaa ggtattctta aaatggatta tgccccatat
    80521 acagctgaaa atgtcgttca aaacgctaat aaattaatag atgtttttag aaaaaacaat
    80581 ggctttatcg cttttgttcg cgtgaatttc tatgatggta aagatgtatt gcaaccaaat
    80641 gcaatgatct cattaccacc aaaagaaggc gacgactaca gtcgtttcca tcatttatta
    80701 gacaagagag atgacgattt tgtcatagac aaacgacatt ttagtgcatt tgtaggaaca
    80761 gatttggact tacaattgcg acgtcgagga attgatacga ttgttcttgg tggtgtcgca
    80821 acgcatattg gcgtagatac gacagcgcga gatgcctatc aattaaacta caatcagttt
    80881 tttgttacag atatgatgag tgcacaaaac gaaacgctac atcaatttcc aatagataat
    80941 gtattcccat tgatgggaca aacaataact acaaacgact ttctaaatat attgaactaa
    81001 acatatactt ccccccttcg atcatgttga gggggatctt tatttcacaa agtattaata
    81061 cgtcgggttg tctaaccttc tatatttaac atattctata tctgttaaat cgttcttaac
    81121 ttacgcccct actacataaa aaacagtatt tattccggaa ttttca
//
LOCUS       DALAGD010000002        48455 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.002, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000002 DALAGD010000000
VERSION     DALAGD010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 48455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48455
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.002"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            435..1139
                     /locus_tag="O2701_000066"
     CDS             435..1139
                     /locus_tag="O2701_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCZ4291986.1"
                     /translation="MSKNINQANSKLNTSNKKLKQAYSKSDSKNMKVIYMPHWLEFYS
                     IAIHSDVTSNKKRYYKSYSRGTVVYVKLGSNIGSEFSGNHFCVILDNKDNKGKETVTI
                     VPLSSKGNKNYLKLNESVLNLTATDLKKQIIDISSKVQALAQKYREIDIKLSTEDKKL
                     LSNLNQKANELYRVIGVYSKHKGKDTYVNISAITTISKRRISKINDSDPTGTIIISED
                     DMNEIEKQLKIKFFSN"
     gene            1532..2068
                     /locus_tag="O2701_000067"
     CDS             1532..2068
                     /locus_tag="O2701_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4291987.1"
                     /translation="MHNNNEFNKKLKDFIGSDKRMALVKGYVNEYKLTTVLRALNNSD
                     YTKGTIHTRSIGNLKQIVDNRIIPKNITQNTIFKLSNLTLEVNLYERNTIYHGDFAIY
                     YPVESALIIETDTPRLINHINNNPVQKIFIITTNDWSFNTLELEKIVDETIIYDLKQE
                     DPKQYEILYKNKHGKLPY"
     gene            complement(2181..3722)
                     /gene="guaA"
                     /locus_tag="O2701_000068"
     CDS             complement(2181..3722)
                     /gene="guaA"
                     /locus_tag="O2701_000068"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCZ4291988.1"
                     /translation="MEMAKEQELILVLDFGSQYNQLITRRIREMGVYSELHDHEISIE
                     EIKKMNPKGIILSGGPNSVYEEGSFTIDPEIYNLGIPVLGICYGMQLTTKLLGGKVER
                     ANEREYGKAIINAKSDELFAGLPAEQTVWMSHSDKVIEIPEGFEVIADSPSTDYAAIE
                     DKKRRIYGVQFHPEVRHTEYGNDLLNNFVRRVCDCRGQWTMENFIEIEIEKIRQRVGD
                     RRVLCAMSGGVDSSVVAVLLHKAIGDQLTCIFVDHGLLRKGEGDMVMEQFGEGFNMNI
                     IRVNAKDRFMNKLKGVSDPEQKRKIIGNEFVYVFDDEASKLKGVDFLAQGTLYTDVIE
                     SGTKTAQTIKSHHNVGGLPEDMEFELIEPINTLFKDEVRKLGIELGIPEHLVWRQPFP
                     GPGLGIRVLGEITEDKLEIVRESDAILRQVIREEGLEREIWQYFTVLPNIQSVGVMGD
                     YRTYDHTVGIRAVTSIDGMTSDFARIDWEVLQKISSRIVNEVDHVNRVVYDITSKPPS
                     TIEWE"
     gene            complement(3747..5213)
                     /gene="guaB"
                     /locus_tag="O2701_000069"
     CDS             complement(3747..5213)
                     /gene="guaB"
                     /locus_tag="O2701_000069"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCZ4291989.1"
                     /translation="MWESKFAKESLTFDDVLLIPAQSDILPKDVDLSVQLSDKVKLNI
                     PVISAGMDTVTESKMAIAMARQGGLGVIHKNMGVEEQADEVQKVKRSENGVISNPFFL
                     TPEESVYEAEALMGKYRISGVPIVDNKEDRNLVGILTNRDLRFIEDFSIKIVDVMTQE
                     NLITAPVNTTLEEAEKILQKHKIEKLPLVKDGRLEGLITIKDIEKVIEFPNAAKDEHG
                     RLLVAAAIGISKDTDIRAQKLVEAGVDVLVIDTAHGHSKGVIDQVKHIKKTYPEITLV
                     AGNVATAEATKDLFEAGADIVKVGIGPGSICTTRVVAGVGVPQITAIYDCATEARKHG
                     KAIIADGGIKFSGDIIKALAAGGHAVMLGSLLAGTEESPGATEIFQGRQYKVYRGMGS
                     LGAMEKGSNDRYFQEDKAPKKFVPEGIEGRTAYKGALQDTIYQLMGGVRAGMGYTGSH
                     DLRELREEAQFTRMGPAGLAESHPHNIQITKESPNYSF"
     gene            complement(5251..6519)
                     /locus_tag="O2701_000070"
     CDS             complement(5251..6519)
                     /locus_tag="O2701_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HCZ4291990.1"
                     /translation="MKNLILSVQHLLAMYAGAILVPIIVGTSLKFTPEQIAYLVTVDI
                     FMCGVATFLQANKVTGTGLPIVLGCTFTAVAPMILIGQTKGIDVLYGSLFLSGILVII
                     IAPFFSHLVKFFPPVVTGSVVTIIGINLMPVAMNYLAGGQGAKDYGDVKNILLGLMTL
                     IIILLLQRFTTGFIKSIAILIGLVLGTIGAGLLGMVDINQVNHAGWLGIPVPFRFSGF
                     SFDVTSTLVFFIVAIVSLIESTGVYHALSEITGKKLERKDFRKGYTAEGLAIVLGSIF
                     NSFPYTAYSQNVGLVSLSGAKKNNVIYGMVVLLLICGCIPKLGALANIIPLPVLGGAM
                     IAMFGMVMAYGVSILGHIDFKNQNNLLIIAVSVGLGTGISAVPQAFKGLGEQFAWLTQ
                     NGIVLGAISAIILNFFFNGIKYKQTEENVK"
     gene            complement(6519..7097)
                     /gene="xpt"
                     /locus_tag="O2701_000071"
     CDS             complement(6519..7097)
                     /gene="xpt"
                     /locus_tag="O2701_000071"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCZ4291991.1"
                     /translation="MELLGQKVKEDGVVIDEKILKVDGFLNHQIDAKLMNEVGRTFYE
                     QFKDKGITKILTIEASGIAPAIMAALHFDVPCLFAKKAKPSTLTDGYYETSIHSFTKN
                     KTSTVIVSKEFLSEEDTVLIIDDFLANGDASLGLYDIAQQANAKTAGIGIVVEKSFQN
                     GHQRLEEAGLTVSSLCKVASLEGNKVTLVGEE"
     regulatory      complement(7328..7430)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            7610..8017
                     /locus_tag="O2701_000072"
     CDS             7610..8017
                     /locus_tag="O2701_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCZ4291992.1"
                     /translation="MADITVVNDTGELYNVINQKKSEGYLESELTIISKSKLHLNDLH
                     DSEISLISTSGTFSDRMTKLLTGEDGEHAVLSRYNLAPDELEKYKQLILDDKMLVVAV
                     RDKSSHQEVHENNSAYEEIDITHFAEASKGPKA"
     gene            8160..8822
                     /locus_tag="O2701_000073"
     CDS             8160..8822
                     /locus_tag="O2701_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4291993.1"
                     /translation="MEFKTIQSVKDPHYNEALKLYDTKLNLSLTEDKRIIKRSLENNK
                     TENDYVFIVGLENDVVVSLATAHYEATTNSAFLIYLFAKDLPNHDELMTQTLHQIEHE
                     LNLLSNLVHSRSINFLMMEVPKEPADLTQDNGLIIEHRRQFLYEHQFERQTEIDYIHP
                     TNDGHTKKVDLFLKKNIKLTKDIYGTSVKSNYILKYVFANGIAREIIYALLEEMDLRK
                     PF"
     gene            8940..9896
                     /locus_tag="O2701_000074"
     CDS             8940..9896
                     /locus_tag="O2701_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4291994.1"
                     /translation="MLTKEFAQRVELSEKQVRKIVQHLEERGYQLSKTEYRGREATDF
                     KEEDIELFKDIADKVKQTNSYDLAFDELEKEKDFLQVIVKNDDKNLPTNQNVAQLVED
                     LRLEIQKMREERHLLGQMMNQVHQQQQELKELQNQLTSKIDSNSESLKAIQTSQEAIQ
                     EAQASQAKALAESTNKVEKNAVTEDKADSKDSKVAGVNTSTDAKTDTKAENAGDGTAT
                     KVDKEDQISATEAIEKASVEQSKNDNAAETSNKEATVDAEAQHDAEQQVAEAHAEASK
                     QATSNDSLEAKAENDSTASQSEMSEPKPQEEKKGFFARLFNL"
     gene            complement(10687..10788)
                     /locus_tag="O2701_000075"
     CDS             complement(10687..10788)
                     /locus_tag="O2701_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4291995.1"
                     /translation="MQLNVNMMKFDRWHKGICKQIVLNSVVIGGKGK"
     gene            10873..12261
                     /locus_tag="O2701_000076"
     CDS             10873..12261
                     /locus_tag="O2701_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HCZ4291996.1"
                     /translation="MNAFLTLINIIVLVIFIVILHMMARKHISFAKRVFTALGIGIVF
                     GVLLHLIYGTHSNVITSTSDWFNIVGQGYVALLQMIVMPLIFISIVAAFTKIQIGEKF
                     AKIGSLIFIFLIGTVTIAAIVGVVYALVFGLDASTINLGNAEQARGSEIAKQAKDLTA
                     HTLPQQILELLPKNPFLDFTGQRATSTIAVVIFASFIGFAYLRVARKQPDHGELLKRA
                     IDAIYSLVMAIVTFVLRLTPYGVLAIMANTLSTSDFGAIWTLGKFLIASYAALITMYI
                     IHLIILSLLGISPIRYVKKTLEVLIFAFTSRSSAGALPLNVQTQTRRLGVPEGIANFA
                     ATFGLSIGQNGCAGIYPAMLAIMVAPVANVEIDLQFIVTLIAVVIISSFGVAGVGGGA
                     TFASILVLSTLNLPVALAGVLISVEPLIDMGRTALNVNDSMLAGTGTAKLTKHWDKDT
                     FDSNDNAALTSH"
     gene            complement(12342..13097)
                     /locus_tag="O2701_000077"
     CDS             complement(12342..13097)
                     /locus_tag="O2701_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HCZ4291997.1"
                     /translation="MSEHVYNLVKKHHSVRKFKNKPLSEDVVKKLVEAGQSASTSSFL
                     QAYSIIGIDDEKIKENLREVSGQPYVVENGYLFVFVIDYYRHHLVDQHAETDMENAYG
                     STEGLLVGAIDAALVAENIAVTAEDMGYGIVFLGSLRNDVERVREILDLPDYVFPVFG
                     MAVGEPADDENGAAKPRLPFDHVFHHNKYHADKETQYAQMADYDQTISEYYDQRTNGN
                     RKETWSQQIEMFLGNKARLDMLEQLQKSGLIQR"
     gene            13589..14158
                     /gene="ahpC"
                     /locus_tag="O2701_000078"
     CDS             13589..14158
                     /gene="ahpC"
                     /locus_tag="O2701_000078"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HCZ4291998.1"
                     /translation="MSLINKEILPFTAQAFDPKKDQFKEVTQEDLKGSWSVVCFYPAD
                     FSFVCPTELEDLQNQYEELQKLGVNVFSVSTDTHFVHKAWHDHSDAISKITYTMIGDP
                     SQTITRNFDVLDEATGLAQRGTFIIDPDGVVQASEINADGIGRDASTLAHKIKAAQYV
                     RKNPGEVCPAKWEEGAKTLQPGLDLVGKI"
     gene            14174..15697
                     /gene="ahpF"
                     /locus_tag="O2701_000079"
     CDS             14174..15697
                     /gene="ahpF"
                     /locus_tag="O2701_000079"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCZ4291999.1"
                     /translation="MLNADLKQQLKQLLELMEGNVEFVASLGSDDKSKELKDLLTEIT
                     DMSPRLSLSEKSLKRTPSFSVNRPGEETGVTFAGIPLGHEFNSLVLAILQVSGRAPKE
                     KQSIIDQIKKLEGSFHFETFISLTCQKCPDVVQALNLMSVINPNITHSMIDGAVFREE
                     SENIMAVPAVFLNGEEFGNGRMTIQDILSKLGSTADASEFENKEPYDVLIVGGGPASG
                     SAAIYTARKGLRTGIVADRIGGQVNDTAGIENFITVKETTGSEFSSNLAAHIDQYDID
                     AMTGIRATDIEKTDEAIKVTLENGAVLESKTVIIATGAGWRKLNIPGEEQLINKGVAF
                     CPHCDGPLFENKDVAVIGGGNSGVEAAIDLAGIVNHVTLFEFASELKADNVLQDRLRS
                     LSNVDIKTNAKTTEVVGEDHVTGIRYEDMNTGEEHLLNLDGIFVQIGLLPNTSWLNDA
                     VELNERGEIVIDRNNNTNVPGIFAAGDVTDQKNKQIIISMGAGANAALNAFDYIIRN"
     gene            15827..16069
                     /locus_tag="O2701_000080"
                     /pseudo
     CDS             15827..16069
                     /locus_tag="O2701_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16142..16768
                     /locus_tag="O2701_000081"
     CDS             16142..16768
                     /locus_tag="O2701_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDxxF motif lipoprotein"
                     /protein_id="HCZ4292000.1"
                     /translation="MKKRLLLSTFLASTLILTGCASDQSDNEDHHTSTGIHAPKSAKK
                     LETKDIFNSDKKNSDISDAEMKQAIEKYLSVNSDILDNKYIMQHKLDKQIDSQTKVTE
                     KQAETLSHLSNLAVKNDLHFKKFVTENNIPKEYKKPVELMMNYFKALNSTIANVDEDI
                     EKLSYQPQNKINVVDVPTKYAGDVNKKQQDKIKDFLKSKGIKSDVIDK"
     gene            17023..17406
                     /locus_tag="O2701_000082"
     CDS             17023..17406
                     /locus_tag="O2701_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292001.1"
                     /translation="MKVRITFIILAILSTIVCLFLAAMHPTGPNTVTFEQPYLFTLNI
                     IIMVLVALPSLVLAIYDYMSFRISSAILQFLGAISWFFLSLILSLTQFTPFTLASFIT
                     SIILFTSTIITLAIGGKSVEKNDSP"
     gene            complement(17472..18053)
                     /locus_tag="O2701_000083"
     CDS             complement(17472..18053)
                     /locus_tag="O2701_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCZ4292002.1"
                     /translation="MTIYLVRHGESKSNYDNKHFRSYFCGQLDVPLTDTGTKSADDLC
                     DYFKEKQIKHVYVSDLLRTQQTFEHIFPYDIASTTTPLLRERSLGVFEGEYKDEISAN
                     PKYDKYFNDPNFKDFRHSFSQKAPEGESYEDVYQRVEHFMNHVVNEDTQKDDIVIVAH
                     QVVIRCLMVYFNKVSRAEAVDLKVENCKPYIIE"
     gene            18231..18440
                     /locus_tag="O2701_000084"
     CDS             18231..18440
                     /locus_tag="O2701_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292003.1"
                     /translation="MTTQSFIFMNIILLDGATQRHPFFICQILAFLFPKFPHINLLLF
                     NPILSTFSNIIIHIDHFHANKKDDN"
     gene            complement(18479..18730)
                     /locus_tag="O2701_000085"
     CDS             complement(18479..18730)
                     /locus_tag="O2701_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCZ4292004.1"
                     /translation="MFGFIGMLIVGGLIGWAAGAIMGKDIPGGILGNIIAGIIGSWVG
                     GKLFGQWGPELGSIYILPALIGSIILIAIVTLILRAMRK"
     gene            19030..19293
                     /locus_tag="O2701_000086"
     CDS             19030..19293
                     /locus_tag="O2701_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ4292005.1"
                     /translation="MNMHILYNLRTKHNLEIDELAQQLNEKYGTKYEAHQIWEWENHH
                     HEPKFKDAMHLADFFDAPYEMFLESKVKEYQKHLEEVDIRMDK"
     gene            complement(19431..20003)
                     /locus_tag="O2701_000087"
     CDS             complement(19431..20003)
                     /locus_tag="O2701_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCZ4292006.1"
                     /translation="MKLKSLAVLSMSAVVLTACGNDTPKDETKSTESNTNQDTNTTKD
                     VIALKDVKTSPEDAVKKAEETYKGQKLKGISFENSNGEWAYKVTQQKSGEESEVLVAD
                     KNKKVINKKTEKEDTMNENDNFKYSDAIDYKKAIKEGQKEFDGDIKEWSLEKDDGKLV
                     YNIDLKKGNKKQEVTVDAKNGKVLKSEQDH"
     gene            complement(20184..20552)
                     /locus_tag="O2701_000088"
     CDS             complement(20184..20552)
                     /locus_tag="O2701_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCZ4292007.1"
                     /translation="MKTILKTITYLVLTIIGAYAALFILKTIDSHGITDQFNPLVKKD
                     DSYVKTTEVSTRMDDQLRSYSQSVFNKEGKETQLMYTATFDVKPHRYLKITHKGHHVE
                     TFEEVEKEEVPKKALDKLSR"
     gene            complement(20921..21532)
                     /locus_tag="O2701_000089"
     CDS             complement(20921..21532)
                     /locus_tag="O2701_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCZ4292008.1"
                     /translation="MFKKYDSKNSIVLKSILSLGIIYGGTFGIYPKADASTQNSSSVQ
                     DKQLQKVEEVPNNSEKALVKKLYDRYSKDTINGKSNKSRNWVYSERPLNENQVRIHLE
                     GTYTVAGRVYTPKRNITLNKEVVTLKELDHIIRFAHISYGLYMGEHLPKGNIVINTKD
                     GGKYTLESHKELQKDRENVKINTADIKNVTFKLVKSVNDIEQV"
     gene            21732..22439
                     /locus_tag="O2701_000090"
                     /pseudo
     CDS             21732..22439
                     /locus_tag="O2701_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505297.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            22478..23416
                     /locus_tag="O2701_000091"
     CDS             22478..23416
                     /locus_tag="O2701_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HCZ4292009.1"
                     /translation="MLNFDEQIAKLKQMNIFFNIIDTEKANEILRKNNYFFKLAYFRK
                     NFEKKNGGYFIEFAYLSDLATIDMKLRYTMLHLTLDIEHSLKYLVLKLITENNQEDGY
                     KIIDEFLCIDKSYSNSNFDTNSRTPEEVMETKIKNKNEIFKHMNKRGQLPEKLNKYYQ
                     NPPAWVCIEFMQLGQFVSFLNFYYKKYNDEELRVANILMPLVKNIRNKSAHNQPIIAN
                     LNYDSRLPQYLFEKGNNIGISRNMFGIKNFIDTFATLELHNQVCSNAIIQARYHDLDQ
                     LQKRYKRNESYYNNALAIKRFFIALDKIIDFNRPKV"
     gene            complement(23682..23924)
                     /gene="rpsR"
                     /locus_tag="O2701_000092"
     CDS             complement(23682..23924)
                     /gene="rpsR"
                     /locus_tag="O2701_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002484306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCZ4292010.1"
                     /translation="MAGGPRRGGRRRKKVCYFTANGITHIDYKDTELLKRFISERGKI
                     LPRRVTGTSAKYQRMLTTAIKRSRHMALLPYVKEEQ"
     gene            complement(23976..24479)
                     /gene="ssb"
                     /locus_tag="O2701_000093"
     CDS             complement(23976..24479)
                     /gene="ssb"
                     /locus_tag="O2701_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCZ4292011.1"
                     /translation="MLNRVVLVGRLTKDPEYRTTPSGVSVATFTLAVNRTFTNAQGER
                     EADFINCVVFRRQADNVNNYLSKGSLAGVDGRLQSRNYENQEGRRVFVTEVVCDSVQF
                     LEPKNAQQNGGQRQQNEFQDYGQGFGGQQSGQNNSYNNSSNTKQSDNPFANANGPIDI
                     SDDDLPF"
     gene            complement(24500..24796)
                     /locus_tag="O2701_000094"
     CDS             complement(24500..24796)
                     /locus_tag="O2701_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCZ4292012.1"
                     /translation="MRTYEVMYIVRPNIEEDAKKALVERFNGILATEGAEVLEAKDWG
                     KRRLAYEINDFKDGFYNIVRVKSDNNKATDEFQRLAKISDDIIRYMVIREDEDK"
     gene            25040..25231
                     /locus_tag="O2701_000095"
     CDS             25040..25231
                     /locus_tag="O2701_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292013.1"
                     /translation="MNLNIDWSKDFQEFQEILNSGIHPEWLYCAKANLVLEPAYTGEG
                     KQFFSTQDIINASKIIPFF"
     gene            complement(25317..26414)
                     /gene="ychF"
                     /locus_tag="O2701_000096"
     CDS             complement(25317..26414)
                     /gene="ychF"
                     /locus_tag="O2701_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCZ4292014.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGALAANYPFATIDPNVGIV
                     EVPDARLLKLEEMVQPKKTLPTTFEFTDIAGIVKGASKGEGLGNKFLSHIREVDAICQ
                     VVRAFDDDNVTHVAGRVDPIDDIEVINMELVLADLESVEKRLPRIEKLARQKDKTAEM
                     EVRILTTIKEALENGKPARSIDFNEEDQKWVNQAQLLTSKKMLYIANVGEDEIGDDDN
                     DKVKAIREYAAQEDSEVIVISAKIEEEIATLDDEDKEMFLEDLGIEEPGLDRLIRTTY
                     ELLGLSTYFTAGVQEVRAWTFKQGMTAPQCAGIIHTDFERGFIRAEVTSYDDYVQYGG
                     ESGAKEAGRQRLEGKEYIMQDGDIVHFRFNV"
     gene            complement(26426..26629)
                     /locus_tag="O2701_000097"
     CDS             complement(26426..26629)
                     /locus_tag="O2701_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HCZ4292015.1"
                     /translation="MASKYGINDIVEMKKQHACGTNRFKIIRMGADIRIKCENCQRSI
                     MIPRQTFDKKLKKIIESHDDTQR"
     gene            complement(26659..27540)
                     /locus_tag="O2701_000098"
     CDS             complement(26659..27540)
                     /locus_tag="O2701_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HCZ4292016.1"
                     /translation="MNQVMNIISSLFEPLTKIETYENIATKIAMIVIYIIVALIVIKI
                     LNKMIEQGFKIQNKSKKSNKKRSKTLISLVQNVVKYIVWFIVITTILSKFGISVEGVI
                     ASAGVVGLAVGFGAQTIVKDVITGFFIIFESQFDVGDYVKINNGGTTVAEGTVKSIGL
                     RSTRINTISGELTILPNSSMGEITNYSITNGTAIVKIPVSVEENIDNVDKKLNKLFTS
                     LRSKYYLFVSDPVVIGIDAIEDTRVILRISAETIPGEGFAGARIIRKEVQKMFLQEGI
                     KTPQPIMTAYNHSENGV"
     gene            complement(27697..28542)
                     /locus_tag="O2701_000099"
     CDS             complement(27697..28542)
                     /locus_tag="O2701_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCZ4292017.1"
                     /translation="MSELSKSEDQRITKTKDEQIKQIDISDIKPNPYQPRKTFDENHL
                     NDLADSIKQYGILQPIVLRKTVQGYYIVVGERRFRASKIAGLKYVSAIIKDLTDEDMM
                     ELAVIENLQREDLNAIEEAESYQRLMTDLKITQQEVAKRLSKSRPYIANMLRLLHLPK
                     KIADMVKDGRLTSAHGRTLLAIKDEQQMLRLAKRVVKEKWSVRYLENHVNELKNVSSK
                     SETDKVDITKPKFIKQQERQLREQYGTKVDISIKKSVGKISFEFDSQEDFVRIIEQLN
                     RRYGK"
     misc_binding    28771..29140
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            29206..30309
                     /locus_tag="O2701_000100"
     CDS             29206..30309
                     /locus_tag="O2701_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCZ4292018.1"
                     /translation="MKDTQLAQITLTDDSTGAIANPIHLSTAYKHPKLGQSTGFDYTR
                     TKNPTRSTFETCFAKLEHGIASFATSSGMSAIQLICNLFKPHDEILVSFDLYGGTFRL
                     FEFYEQQYDIKFKYVDFTDYEQVEKEITDKTVALFIEPISNPQMIAIDVKPYYQLCKA
                     KGLLSIIDNTFLTPYLSTPLAEGADIVLHSATKYIGGHNDVLAGVVTVKDESLAQQLF
                     DFHNMTGATLSPIDSYLLLRGLKTLHLRIERAQSNARKLAKKCQSLQAIDEVLYSGQT
                     GMLSLRLNKAYSVAKLLENLDICIFAESLGGTETLVTFPYTQTHVDMPDAEKDKRGID
                     EYLIRLSLGVENYEDIERDIIQALDKAQIGEIV"
     gene            30306..31466
                     /locus_tag="O2701_000101"
     CDS             30306..31466
                     /locus_tag="O2701_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HCZ4292019.1"
                     /translation="MTLSKETEVIFDWRRGVEYHSANPPLYDSSTFHQTSLGGDVKYD
                     YARSGNPNRELLEEKLARLEQGKFAFAFASGIAAISAVLLTFKSGDHVILPDDVYGGT
                     FRLTEQILNRFNIEFTTVDTTKLEQIEGAIQSNTKLIYIETPSNPCFKITDIKAVSKI
                     AEKHELLVAVDNTFMTPLGQSPLLLGADIVIHSATKFLSGHSDLIAGAVITNNEAISD
                     ALYLIQNGTGNMLSAQDSWTLAKHLKTFPIRFKQSVENAQKIVSFLIKQDEISEVYYP
                     GLTTAHLEQAKNGGAVIGFRLADESKAQQFVDALTLPLVSVSLGGVETILSHPATMSH
                     AALPEEVRQERGITFGLFRLSVGLEDPDELIADIKYALKEAFNESIPHTIER"
     gene            31435..33276
                     /locus_tag="O2701_000102"
     CDS             31435..33276
                     /locus_tag="O2701_000102"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HCZ4292020.1"
                     /translation="MSQFLTQLKDNVLVADGAIGTILYSEGLDTCPEAYNLSHPDKVE
                     RIHRSYIEAGADVIQTNTYGANFEKLKRFGLEDKVKAIHQAAVRIAKKAANKDTYILG
                     TVGGFRGIKQEDISLQTILYHTEIQIDTLIEEGVDALLFETYYDLEELTNVISRTRKK
                     YDIPIIAQLTASNTNYLVNGQAINEGLKQLVQCGANIVGLNCHHGPHHMQESFTHIEL
                     PEHAFLSCYPNASLLDIENSEFKYSDNAQYFGQVAQNLIREGVRLIGGCCGTTPEHIK
                     FIKESIQTLKPVNDKKVIPIPTKALFNPSQNKVRQSLTSKVQERPTVIIELDTPKHLD
                     TDRFFENIAKLDKANVDAVTLADNSLATVRISNIAAASLIKQYYNIEPLVHITCRDRN
                     LIGLQSHLLGLSLIGVNEILAITGDPSKVGHLPGATNVYDVNSKGLTELALRFNQGIN
                     TDGDALKKRTHFNIAGAFNPNVRKLYGAVKRLEKKIESGMSYFITQPVYSKEKIIEIY
                     HATKHLNKPFFIGIMPIASYKNALFLHNEVPGIKMSDEILQQFEAVKDDKAKTRELSL
                     KLSKDLIDTVHEYFNGLYIITPFQNVEDSLELAAYSKSITAHKEAIL"
     gene            33273..35501
                     /gene="metE"
                     /locus_tag="O2701_000103"
     CDS             33273..35501
                     /gene="metE"
                     /locus_tag="O2701_000103"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCZ4292021.1"
                     /translation="MTTIKTSNLGFPRLGRKREWKKAIESYWAKKISKEELDQTLTDL
                     HKENLLLQKYYHLDSIPVGDFSLYDHILDTSLLFNIIPERFQGRTIDDDLLFDIARGN
                     KDHVASALIKWFNTNYHYIVPEWDNVEPKVSRNVLLDRFKYAQSLNVNAHPVIVGPIT
                     FVKLSKGGHQTFEEKVKTLLPLYKEVFESLIDAGAEYIQVDEPILVTDDSESYENITR
                     EAYDYFEKAGVAKKLVIQTYFERAHLKFLSSLPVGGIGLDFVHDNGYNLKQIEAGDFD
                     KSKTLYAGIIDGRNVWASDIEAKKVLIDKLLAHTNELVIQPSSSLLHVPVSLDDETLD
                     TSVGEGLSFATEKLDELDALRRLFNQNDSVKYDKLKARYERFQNQSFKNLDYDFESVR
                     TSRQSPFAQRIEQQQKRLNLPDLPTTTIGSFPQSREVRKYRADWKNKRITDEAYETFL
                     KNEIARWIKIQEDIGLDVLVHGEFERNDMVEFFGEKLQGFLVTKFGWVQSYGSRAVKP
                     PIIYGDVKWTAPLTVDETVYAQSLTDKPVKGMLTGPVTILNWSFERVDLPRKVVQDQI
                     ALAINEEVLALEAAGIKVIQVDEPALREGLPLRSEYHEQYLKDAVLSFKLATSSVRDE
                     TQIHTHMCYSQFGQIIHAIHDLDADVISIETSRSHGDLIKDFEDINYDLGIGLGVYDI
                     HSPRIPTKEEITTAINRSLQQIDRSLFWVNPDCGLKTRKEEEVKDALTVLVNAVKAKR
                     QE"
     gene            35544..36296
                     /locus_tag="O2701_000104"
     CDS             35544..36296
                     /locus_tag="O2701_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HCZ4292022.1"
                     /translation="MTQYPLWQQLNTLKQATWVDLTHTFDPEIPRFSEFEKGEVSTLF
                     TVKDHGFYVQRWNIVTQYGTHIDAPIHFVENKRYLEDIDLKELVLPLIVLDFSTEVAN
                     NNDFIVTRAHIEAWEKEHGTIEPGTFVALRTDWSKRWPNIEKFENKDANGQQHAPGWG
                     LDALKYLIEERRVEAVGHETFDTDASVDVVKNGDLVGERYILGQDKYQVELLTNLDQL
                     PTRGAIIYAISPKPKDAPGFPVRAFAIKPSND"
     gene            complement(36379..37560)
                     /locus_tag="O2701_000105"
     CDS             complement(36379..37560)
                     /locus_tag="O2701_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCZ4292023.1"
                     /translation="MTRVVLAAAYRTPIGVFGGAFKDVPAYDLGATLIEHIIKETGLN
                     PSEIDEVIIGNVLQAGQGQNPARIAAMKGGLPETVPAFTVNKVCGSGLKSIQLAYQSI
                     VTGENDIVLAGGMENMSQSPMLVNNSRFGFKMGHQSMVDSMVYDGLTDVFNQYHMGIT
                     AENLVEQYGISREEQDTFAVNSQQKAVRAQQNGEFDSEIVPVSIPQRKGEPIVVTKDE
                     GVRENVSVEKLSRLRPAFKKDGTVTAGNASGINDGAAMMLVMSEDKAKELNIEPLAVL
                     DGFGSHGVDPSIMGIAPVGAVEKALKRSKKELSDIDVFELNEAFAAQSLAVDRELKLP
                     PEKVNVKGGAIALGHPIGASGARVLVTLLHQLNDEVETGLTSLCIGGGQAIAAVVSKY
                     K"
     gene            38099..39232
                     /locus_tag="O2701_000106"
     CDS             38099..39232
                     /locus_tag="O2701_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HCZ4292024.1"
                     /translation="MVNALTPKHLAEKRAGFHELFFDLIFVYAIQKIAHVILTTQNGS
                     ISADLFFKYIVMSLFLWLMWSHQTFFTNRFGQVTFKDVSFMMFNMFIMVFLSNSLYPD
                     FEKTFFPFFLCVAIMYLSIGLQYLLHIRTGLDYGDKRTCQAFASVAFVISFLSFLSLV
                     LPQSIHYIPGFLGVFIAATGLIPFQKYLVLSPVNMMHLVERFSLLTIIIFGEVLVGLA
                     SSSFSIDHFSYIYIFQFMILISLFGVYWIITENYINHKLTSIGFRLSYTHLLINIALG
                     VINAAIVFSNNNKLNDLFEINMMYISVLIFYIGLWLITPYFHNELTNAKYISYSLGIL
                     VMSYIVSLLFKGHDHVMIISVSVATFCIMLIYFKNQRLRNKHE"
     gene            complement(39599..40228)
                     /locus_tag="O2701_000107"
     CDS             complement(39599..40228)
                     /locus_tag="O2701_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HCZ4292025.1"
                     /translation="MKGMFLSSFYATRKQTYIYFIVAIIAAGYFAVFNPLMSSAMAGV
                     MLITPITDNIKHEKDSRWMYYVSTLPVKRSDYIKSYFAFYLILFGASLMIGLVVTTIV
                     TQSVMIGIMSGLMSFGIIGAYSIIFPLTFKFGAENSNVIMICASILLLISFVVFFFIY
                     GMVSGASALEFEKISTEGWLVVIAYAVIGIVITSVSYILSIKIFNKQEL"
     gene            complement(40228..41070)
                     /locus_tag="O2701_000108"
     CDS             complement(40228..41070)
                     /locus_tag="O2701_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4292026.1"
                     /translation="MTTLLNVDSVNKQYKDSDFKLQDASLTISTNETVGLIGKNGSGK
                     STLINILVGNRHKDNGSITFFGEEHTVDDVEYKEHIGVVFDDLRVPNKLTIKDIDKVF
                     QSIYMTWNSQKFFDLIKYFELPLQTKIKTFSRGMRMKIALTIALSHDVKLLILDEATA
                     GMDVSGREEVMEILEDFVAQGGGILISSHISEDIEHLADKLVFMKDGRMILTEQKDIL
                     LAQYGIVTTEDKDVEIPKHLIIASRLSKGKYQILVKDYAEIENAEPLKHIDDATKIIM
                     RGEV"
     gene            complement(41095..41808)
                     /locus_tag="O2701_000109"
     CDS             complement(41095..41808)
                     /locus_tag="O2701_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HCZ4292027.1"
                     /translation="MKILRYIGYLLLGGLVGGIIGGILGNFDGFGIENLTFATYNNVV
                     VISIVATIIIILVEAIVLMNQRRALKYKQLVDKEVDIDATDQYELLANRYVLNGSILS
                     ILQTVIAFLVLLIFVVGHAAANAILFFLIPFFASAIFNTQFTLFNRRFDDRIPKIADK
                     NYTEKRLEILDEGERHIELIALFKTYAINLSILILAIVVIGLYSITTGINQSFSLLLI
                     IAIFIYNAFSYLLKRRRFY"
     gene            complement(41805..42008)
                     /locus_tag="O2701_000110"
     CDS             complement(41805..42008)
                     /locus_tag="O2701_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ4292028.1"
                     /translation="MRNRLKELRARDGLNQTQLAKQAGVSRQTISLIERNNFMPSVLT
                     AIKIARIFNETVETVFIIEEDEA"
     gene            42249..42638
                     /locus_tag="O2701_000111"
     CDS             42249..42638
                     /locus_tag="O2701_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HCZ4292029.1"
                     /translation="MAFTLSAIQQAHQQFTGVDFPKLFKAFKDMGMTYNIVNIQDGTA
                     TYVHQSEDDIVTSSVKSNHPVAQKSNKTIVQDVLTRHQQGQTDFETFCDEMAEAGIYK
                     WHIDIQAGTCTYIDLQDQAVISELIPQ"
     gene            42738..42962
                     /locus_tag="O2701_000112"
     CDS             42738..42962
                     /locus_tag="O2701_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HCZ4292030.1"
                     /translation="MIIMITNTFILGITGPTSLVVISIIALIIFGPKKLPQFGRAIGS
                     TLKEFKSATEDLDKESHDTPSKESKQQREQ"
     gene            42979..43680
                     /gene="tatC"
                     /locus_tag="O2701_000113"
     CDS             42979..43680
                     /gene="tatC"
                     /locus_tag="O2701_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HCZ4292031.1"
                     /translation="MVHFSELRHRLVKILLSFVITVIVVYVSSFWWMTPFITYITRAH
                     VSLHAFSFTEMIQIYVMIIFFIAFCFISPVMFYQLWAFIAPGLHNNERQFIYKYSFFS
                     VLLFCAGVAFAFYVGFPMIIQFALKLSLTLNISPVIGFKAYLIELIRWLFTFGLLFQL
                     PILFMGLAKFGLIDTTSLKHYRKYIYFACFVLASIIAPPDLTLNILLTLPLILLFEFS
                     MFIVKFTCRGKPPTH"
     gene            complement(43772..45484)
                     /locus_tag="O2701_000114"
     CDS             complement(43772..45484)
                     /locus_tag="O2701_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCZ4292032.1"
                     /translation="MKHYLTKFVAMLITAAMVCSFGLLKSQAAEQQSISDVYSVITDA
                     KSALSNNSISNDNKQKAIEQVVSAVKKLSLEDNSESNAVKSDVRKLEDAKANDNQKDT
                     LSQLTKSLIAYEEKLASKDAGSKIKLLQQQVDAKDAAMTKAIKDKNKAELESLNNSLN
                     QIWTSNETVIRNYDANQYGQIEVALLQLRIAIHKSPLDTAKVSHAWTTFKSNIDHVDK
                     KSNTSANDQYHVSQLNDALEKAIKAIDDNQLSDADAALTHFIETWPYVEGQIQTKDGA
                     LYTKIEDKIPYYQSVLDEHNKAHVKDGLVDLNNQIKEVVGHSYSFVDVMIIFLREGLE
                     VLLIVMTLTTMTRNVKDKKGTASVIGGAIAGLVLSIILAITFVETLGNSGILRESMEA
                     GLGIVAVILMFIVGVWMHKRSNAKRWNDMIKNMYANAISNGNLVLLATIGLISVLREG
                     VEVIIFYMGMIGELATKDFIIGIALAIVILIIFALLFRFIVKLIPIFYIFRVLSIFIF
                     IMGFKMLGVSIQKLQLLGAMPRHVIEGFPTINWLGFYPSYEPLIAQGAYIMVVAILIF
                     KFKK"
     gene            complement(45465..46694)
                     /gene="efeB"
                     /locus_tag="O2701_000115"
     CDS             complement(45465..46694)
                     /gene="efeB"
                     /locus_tag="O2701_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCZ4292033.1"
                     /translation="MTNYEQVNDSTQFSRRTFLKMLGIGGAGVAIGASGVGSMWSFKS
                     MFNTPEDPEKDAYEFYGKVQPGITTPTQKTCNFVALDLKSKDRDAIKAMFKKWTVMAD
                     RMMDGDTVGKPSNNPLMPPVDTGESIGLGASKLTITFGISKSLMKKIGLSSKIPDAFK
                     DLPHFPNDQLIDDYSDGDIMIQACSNDSQVSFHAVHNLVRPFRDIVKVRWAQSGFISA
                     KGKETPRNLMAFKDGTINPRKNNQLKDYVFIDDGWAKHGTYCVVRRIQIHIETWDRTA
                     LEEQEATFGRKRHSGAPLTGGKEFDEIDLKAKDSHGEYIIDKDAHTRLAKEANTSILR
                     RAFNYVDGTDDRTGNFETGLLFIAFQKATKQFIDIQNNLGSNDKLNEYITHRGSASFL
                     VLPGVSKGGYLGETLFD"
     gene            complement(46691..47545)
                     /locus_tag="O2701_000116"
     CDS             complement(46691..47545)
                     /locus_tag="O2701_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292034.1"
                     /translation="MKKLTTLLLASTLLIAACGNDDSKKDDSKTSKKDDGVKAELKQA
                     TKAYDKYTDEQLNEFLKGTEKFVKAIENNDMAQAKALYPKVRMYYERSEPVAEAFGDL
                     DPKIDARLADMKEEKKEKEWSGYHKIEKALYEDKKIDDVTKKDAQQLLKDAKELHAKA
                     DTLDIIPKLMLQGSVDLLNEVATSKITGEEEIYSHTDLYDFKANVEGAQKIYDLFKPI
                     LEKKDKKLSDDIQMNFDKVNQLLDKYKDNNGGYESFEKVSKKDRKAFADAVNALGESL
                     SKMAVITE"
     gene            complement(47813..48358)
                     /locus_tag="O2701_000117"
     CDS             complement(47813..48358)
                     /locus_tag="O2701_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4292035.1"
                     /translation="MFGMKVNEQITLKILEAHDTEALFNLVNRSRNSLREWLPWVDAT
                     EQPSDTRAFIKRGLLQFADGNGFQCGIWYEGTLVGVIGLHEINHMHRKTSLGYYLDKE
                     FEGHGIMTQAVEALIKYCFEELDLNRIEISAAVNNEKSRAIPERLGFTREGMLRDNEL
                     LNGIYSSSYIYSLLKSEYDQK"
BASE COUNT        16014 a         9016 c         7222 g        16203 t
ORIGIN      
        1 catgatgttt taaagcaaat tttaaagttg atttagaaac tgaacttttt aaagatacat
       61 tacttgaaga ttctaattta tctgattata taaagaacca acttccaacg atatggagag
      121 actaagaaat agtttaaatt tatttaattt caataaaaac tcataataat atttaagtaa
      181 gttagattgc ttttagcatt aggaattcgc ttataattag atgttcaata tagacttttt
      241 tacacataca tgaactataa aaagcgaata aatagcaggt cattgcgaaa tttgctaaat
      301 acaaaattga tgagttccac ctaataaaaa ttgttggttt tatagtaaca tctattttcg
      361 taccattcat gttaatttca gtgctaataa tgctgatata aagaagaata aattgattag
      421 gagaacacta tttaatgtct aaaaatatca atcaagcaaa tagtaaatta aatacgtcaa
      481 acaaaaaact aaaacaagct tattcaaaat ccgacagtaa aaacatgaaa gttatataca
      541 tgcctcattg gctagagttt tattcaattg ctatacatag cgatgttaca agcaataaaa
      601 aaagatatta taaatcatat agtagaggaa cagtggtata tgtaaaacta gggagtaata
      661 ttggaagtga gttttctggt aatcattttt gtgtaatatt agataacaaa gacaataaag
      721 gaaaagaaac agttacaata gttccacttt cttccaaagg taataaaaat tatttgaagt
      781 taaacgaatc ggtgctaaat ctaacagcaa cagatttaaa aaaacagatt atcgatatca
      841 gtagtaaagt acaagcatta gcccaaaaat atagagaaat tgacattaaa cttagcacag
      901 aagataaaaa gcttttgtct aacttaaatc aaaaagctaa tgaactgtat agagtgatag
      961 gtgtttattc caagcacaaa gggaaagata catacgttaa tatatcagca attacaacaa
     1021 taagcaaaag acgtataagc aaaataaatg attctgatcc tactggaacg ataataattt
     1081 cagaagatga catgaatgaa atcgaaaagc aattaaagat aaagttcttt tctaattgaa
     1141 ttgtaatatt acatctgata taattgattt acaaattccc ggtaaccatt ccggcttcat
     1201 tctcttgaag aatgacatat tctcagcgtt ttagctgaag gtcagatgat acgtcatctg
     1261 gcctcttttt ttatataaat gccctttaag tgaaatatac aacaatctgc aatcaatatt
     1321 agacatcaca aaacacacaa taaattacac aatgtgttat aagccacatg tcactattat
     1381 cgcagctagc tgcgtcactc aaaacgaata cggaacgcgt tggttatttc aatcataata
     1441 ttactctgca aatacacagc cctgtaaccg agtaaatgga tagagatttg aacaaatgaa
     1501 aacaatcaac taatggaaag gataaaatat tatgcacaac aataatgaat tcaacaaaaa
     1561 actgaaagat tttataggta gcgataaacg gatggcttta gtaaagggtt atgttaacga
     1621 gtataaatta acaactgttt taagagcttt aaataattcc gattacacaa aagggacgat
     1681 acacactcgt tcaataggca acctaaaaca aatagtagat aatcgaatta ttcctaaaaa
     1741 tataactcaa aacacaatct tcaaattgag taatttaacc ttagaggtta acctctatga
     1801 aagaaacaca atttatcacg gagactttgc tatttactat cctgtagaat ctgctctaat
     1861 aatagaaaca gatactccta ggctaattaa tcatataaat aacaacccag tccaaaaaat
     1921 atttataata acaacaaatg actggagctt taatacactt gaattagaaa aaatagtaga
     1981 cgaaactata atttatgatt taaaacaaga agatcctaaa caatatgaaa ttttatataa
     2041 aaataaacac ggaaaattgc cttattgatg aattaaaacg caaacttaga acaccttacc
     2101 ccttttccgt ccttattgta attttccaac tactcaaatt aaaaaccacc cgtgcaaaca
     2161 ggtggtttta ttatatataa ttattcccac tcaattgtgc ttggtggttt tgatgtaatg
     2221 tcatagacta cgcggttgac gtgatctact tcgtttacga tacgactaga aatcttttgt
     2281 aagacttccc aatcgatgcg tgcgaagtca cttgtcatac cgtcgataga tgttactgca
     2341 cgaataccta ctgtgtgatc atacgtacgg tagtctccca taacacctac tgattgaatg
     2401 tttggtaaca ctgtgaagta ttgccaaatt tctctttcaa gaccttcttc tctaatcact
     2461 tggcgtaaaa tcgcgtctga ttctctaacg atttctagtt tatcttcagt aatttctcca
     2521 agtacacgaa taccaagacc aggtcctggg aatggttgtc tccatactaa atgttctgga
     2581 atacctaact caatacctaa tttacgtact tcatctttaa acaatgtatt gattggttcg
     2641 attaattcga attccatgtc ttctggtaat ccaccaacat tgtggtgtga tttgattgtt
     2701 tgtgctgttt tagtacctga ttcgatgacg tctgtatata gtgttccttg cgcaaggaag
     2761 tctacacctt tcagttttga tgcttcatca tcaaatacgt atacaaattc attaccaatg
     2821 attttacgtt tttgttcagg atctgaaaca ccttttaatt tattcataaa gcgatctttc
     2881 gcattaacac gaataatatt catgttgaaa ccttcaccga attgctccat aaccatgtcg
     2941 ccttcacctt tacgaagtaa gccatggtct acaaagatac atgttagttg atcacctatt
     3001 gctttatgca atagtacagc tacaacagat gaatctacgc cgccactcat cgcacataat
     3061 acacgacggt ctcctacgcg ttgacgaatc ttttcaattt cgatttcgat aaagttttcc
     3121 attgtccatt gacctctaca atcacaaaca cgacggacaa aattatttaa taaatcatta
     3181 ccatattctg tatgacgtac ttctggatgg aattgaacac cataaatgcg acgtttctta
     3241 tcttcgattg ctgcatagtc tgtgcttggg ctatcagcga taacttcaaa gccttctgga
     3301 atttcaataa ctttatcaga atgactcatc caaacagttt gttctgctgg taagccagcg
     3361 aataactcat ctgacttcgc attaatgatt gctttaccgt attcacgttc attggcacgt
     3421 tcaactttac cacctaataa tttagtagtt aattgcatgc cgtaacaaat accaagtact
     3481 ggaattccta aattatatat ttccggatca attgtaaatg aaccttcttc ataaactgaa
     3541 tttggaccac ctgataagat aatacctttt ggattcattt tcttaatttc ttcaattgaa
     3601 atttcatgat cgtgtaattc actataaacg cccatttcac gaattcggcg tgtaattaat
     3661 tggttgtatt ggctaccaaa gtctaagaca aggattaact cttgttcttt tgccatttcc
     3721 atatttgtcg ttctccttta tcttaattag aatgagtagt tcggtgattc tttcgtaatt
     3781 tgaatattat gtggatggct ttctgctaaa ccagcaggac ccatacgtgt aaattgtgct
     3841 tcttcgcgta attctcttaa atcgtgtgaa ccagtataac ccataccagc acgcacaccg
     3901 cccattaatt ggtaaattgt atcttgtaac gcacctttat aagccgtacg tccttcgata
     3961 ccttcaggaa caaatttctt aggcgctttg tcctcttgga agtaacggtc gtttgaacct
     4021 ttttccatcg cacctaaaga gcccatacca cggtatactt tatattgtct accttggaaa
     4081 atttctgttg cgcctgggct ttcttcagta cctgctaata agctacctaa cataaccgca
     4141 tgtccaccag cagctaatgc tttaatgata tctcctgaga atttaatacc accatcagca
     4201 atgatagctt taccatgttt gcgtgcttca gttgcacaat cataaattgc tgtaatttgt
     4261 ggtacaccaa cacctgctac aacacgcgtc gtacaaattg aacctgggcc aataccaact
     4321 ttaacaatat ctgcacccgc ttcaaataaa tcttttgttg cttctgcagt tgctacgtta
     4381 cctgctacta atgtgatttc tgggtaagtc ttcttaatat gtttcacttg atcgataaca
     4441 cctttagagt gaccatgtgc tgtatcgata actaagacat ccacacctgc ttcgactaat
     4501 ttttgagcac gaatatcagt atcttttgaa ataccaattg ctgcggctac aagtagacga
     4561 ccatgttcat cttttgctgc attagggaat tcgataactt tttcaatatc tttaatagta
     4621 ataagacctt ctagacgtcc gtctttaact aatggtaact tttcaatctt atgtttttgg
     4681 agaatttttt ctgcttcttc aagtgttgta ttcactggag ctgtaattaa attttcttgc
     4741 gtcattacat ctacaatttt aatcgagaag tcttcaataa aacgtaagtc acggtttgtt
     4801 aaaataccta ctaagttgcg atcttcttta ttatcaacaa ttggtacacc tgaaatacgg
     4861 tatttaccca ttaatgcttc tgcttcataa acgctttctt ctggcgttaa gaaaaatggg
     4921 tttgaaatga caccattttc tgagcgtttt actttttgaa cttcgtccgc ttgttcttca
     4981 acgcccatat ttttatgaat aacacctaaa ccaccttgac gagccatagc aatcgccatt
     5041 ttagattcag ttacagtatc cataccagca gaaataactg gaatatttaa tttaactttg
     5101 tctgataatt gtacgcttaa atcaacgtct ttcggtaaaa tatcagattg tgctggaatt
     5161 aataacacat catcaaacgt taatgattct tttgcaaatt tactttccca cattaaaaac
     5221 agcctccatt tttcaaatta attagttata ttatttcaca ttttcttccg tttgtttata
     5281 ctttattcca ttaaaaaaga aattaagaat aattgcagag attgcgccta aaacaattcc
     5341 gttttgagtc aaccatgcaa attgttcacc taaaccttta aatgcttgtg gtacagcgct
     5401 tataccagta cctaatccta ctgatacagc gataattaat aaattgtttt gatttttaaa
     5461 atcgatatgt cctaatatac taacaccata tgccattacc atgccaaaca tagctatcat
     5521 cgcaccgcct aacacaggta gcggtatgat atttgctaat gcgccaagct taggtataca
     5581 accacatata agtaataaca cgaccatgcc gtatataaca ttgtttttct tagcgccgga
     5641 taaagaaaca agtcctacat tttgcgaata ggctgtatac ggaaatgaat tgaatataga
     5701 acctaacact atcgctagac cttccgcagt ataaccttta cgaaaatctt ttctttctaa
     5761 cttcttaccg gtaatttcac ttaacgcatg atagacacct gtcgactcaa ttaaactaac
     5821 gatagctaca ataaagaaca ctaacgtcga tgtcacatca aagctaaatc cagagaatct
     5881 aaacggcact gggatgccta accaaccggc atgattgact tgattaatat cgaccatccc
     5941 aagtaagcca gcacctatcg ttcctaaaac gagtccaatt aatatggcaa tactcttaat
     6001 aaatccagtt gtgaatcttt gtaaaagaag aataatgatt aatgtcatta aacctaacaa
     6061 aatgttctta acatctccat agtcctttgc accttgacct ccagctaagt aattcattgc
     6121 tactggcatt aaattgatac caatgatagt aacaacacta cccgttacta ctggtgggaa
     6181 gaattttaca agatgtgaaa agaaaggcgc gatgataata actaatatcc ctgataaaaa
     6241 tagcgaacca taaagtacat ctattccttt cgtttgacca attaaaatca tgggcgcaac
     6301 agccgtgaat gtacatccaa gaacgattgg taatcctgtt cctgttactt tattggcttg
     6361 taaaaatgtg gcaaccccac acataaatat atctactgta actaagtaag cgatttgttc
     6421 aggtgtaaac ttcaaacttg taccaacaat gattggaact aagatagcac ctgcgtacat
     6481 agctaaaaga tgttgaacac ttaggattaa atttttcatt attcttctcc caccaatgtc
     6541 actttgtttc cttctagtga agcaaccttg cagagagaag aaactgttaa acctgcttct
     6601 tctaaacgtt gatgcccatt ttggaaactc ttttcaacaa caataccaat accagctgtc
     6661 ttagcattcg cttgctgtgc gatatcgtat aatcctaatg aagcatcacc atttgctaaa
     6721 aagtcatcga tgataagtac agtatcttct tctgataaaa actcttttga aacaatgacc
     6781 gtacttgttt tatttttagt aaatgaatga atagatgttt cataataacc atccgtcaaa
     6841 gtgctaggtt ttgctttttt cgcaaataaa catggcacat caaaatgcag tgcagccatg
     6901 attgcaggtg cgataccgga agcttcaatg gttaagattt tagtaatccc tttatcttta
     6961 aattgctcgt aaaaagtgcg accaacttca ttcattaact ttgcatcaat ttgatgattt
     7021 aaaaatccat cgacttttaa aatcttctca tcaatgacaa cgccgtcttc ctttactttt
     7081 tgtcctagta actccactct taaaacctcc tcagatttgt gtgaaacatt ttgctcttaa
     7141 attggtgcta gatacaaaaa aatccccaaa ctaaataata gtttcagggt ttatgagtga
     7201 acgaacatgc ataacgaatt tgtcatgcaa tcaatgtaaa agaagtttca tcaaataaac
     7261 tgtgaccatc atataaaatg atataaatca cccaccatgg ttacaattta atggctgaag
     7321 ctactcctag tattgtgttg ttactcatag tcatgtcgtt caaggcaaca tggtagaaac
     7381 ttctaaagcc atattcttta gattatatga gtttatgtaa attatttaac gataatagca
     7441 aattttcggc attttttcaa taactgctta ggtaatcttt taatagtttt ataaatgtac
     7501 agagacgttt aatgcgaatg aagacttata acttaataat gtaagggttt tactttataa
     7561 taagaagggt aaaagattaa ataaacaaat ttttaaaggg gcgacaacta tggcagatat
     7621 tactgtagta aatgacactg gcgaattata caacgtaatc aatcaaaaga aatctgaggg
     7681 ttatttagaa tcagagctaa caattattag taaaagtaaa ttacacctaa atgacttaca
     7741 cgactctgaa atctccctta tctcaacaag tggaacattt agtgatagaa tgactaaact
     7801 gttaacagga gaagatggtg aacatgcagt tttatcacgc tacaacttag cgcctgatga
     7861 attagaaaaa tataaacaac ttattttaga cgataaaatg ttagttgtag cggttcgtga
     7921 caaatcatca catcaagaag tgcatgaaaa taattcagct tatgaagaga ttgatattac
     7981 acattttgct gaagcatcta aaggtcccaa agcttagtta tcacactgaa ttcaaaatga
     8041 gattactgta ctgtctaatt taagatagta cagtttttta taccatttaa aagtttatga
     8101 acagtctttt acagcaggcg attatgctat aatattattt aaattgggga ggtgttctta
     8161 tggaatttaa aaccattcaa tctgtcaaag atccacatta caacgaagca cttaaacttt
     8221 acgatactaa gttaaattta tctttaactg aagataaacg tatcatcaaa cgctcattag
     8281 aaaacaacaa aaccgaaaac gattatgtct ttatcgtagg cttagaaaac gatgttgttg
     8341 tcagtctagc aactgcacat tatgaagcca caaccaattc agcattttta atttatttat
     8401 tcgcaaaaga cctacccaac catgatgagt taatgacgca aacattacat caaattgaac
     8461 atgaactaaa cttattatca aaccttgttc attctagaag tattaacttt ttaatgatgg
     8521 aagtccctaa agaacctgca gatttaacac aagataacgg cctcattatt gaacaccgta
     8581 gacagttttt atacgaacat caatttgaaa gacaaactga aattgattac attcatccaa
     8641 ctaatgatgg tcacactaaa aaagtggact tatttttaaa gaaaaatatt aaactgacaa
     8701 aggatatcta cggtacaagt gttaaatcaa attatatttt gaagtatgta tttgccaatg
     8761 gtatcgcacg tgaaattatt tatgctttat tagaagaaat ggatttacgt aaaccatttt
     8821 aaacaaactt gaaatcacta acaataacgt ttaaactatg tatgaagcaa gactgacgtt
     8881 tcactgcccg tagacaaacg tcagtataca taaagtatat gcagaaagga ctttaaagta
     8941 tgttaactaa agaatttgca caacgcgtag aactaagtga gaagcaagta cgtaaaattg
     9001 ttcaacattt agaagaacgt ggttaccaac taagcaagac tgaatatcgt ggtcgtgaag
     9061 caactgattt caaagaagaa gatatcgagc ttttcaaaga cattgccgac aaagtaaaac
     9121 aaacaaatag ttatgatcta gcgtttgatg aattagaaaa agaaaaagac ttcctgcaag
     9181 tcattgtcaa aaacgatgac aaaaacttac ctactaatca aaatgtcgct caactagtag
     9241 aagatttacg cctagaaatc cagaaaatgc gcgaagaacg tcacctactt ggtcaaatga
     9301 tgaatcaagt acatcagcaa caacaagaat taaaagaact tcaaaatcaa cttacatcta
     9361 aaatcgattc aaatagcgaa tccttaaaag ccatccaaac atcacaagag gctatccaag
     9421 aagcgcaagc ctctcaagca aaagcattag ctgaatcaac aaataaagtt gaaaagaatg
     9481 ctgttactga agataaagct gacagtaaag attcgaaggt tgctggagtg aatacatcaa
     9541 cagacgctaa gacggatact aaagcagaaa atgcaggcga tggcactgct actaaagtag
     9601 ataaagagga tcaaatttct gcaactgaag caattgaaaa agccagcgtt gagcagtcta
     9661 aaaatgacaa tgcagctgag acttcaaata aagaagctac agtagatgca gaagcgcaac
     9721 atgatgctga acaacaagta gcagaagccc atgcagaagc ttctaaacaa gctacttcga
     9781 acgattcatt ggaagcgaaa gcagaaaatg acagcacagc ttcacagtca gaaatgtcag
     9841 aaccaaaacc tcaagaagag aaaaaaggtt tcttcgcacg tttattcaac ctgtaaactt
     9901 gtgatttgaa cacaaattta atactcaatt catatatagc tttgatttaa aaactgattt
     9961 ttataaacct atagaactgt aatttacttt caaatttaat actcaattca taaaacctga
    10021 aacaagctga tatactccat tcaatatcta ctttgaagag agcatatcaa actaatttgt
    10081 ttcaggtttt ttaattattc attatgatta atattgaatt ttgaataaat ttttatagta
    10141 tgttactttt aaatgggatg gcttctgtgt acttctaaat agattcatct taataagtcc
    10201 attcaattta aatgtttggc tgtgttagta actgacgaat cgcacaagtg cacactcata
    10261 ttcacattta ctacaaacgc ctttttgctc aaacaaaaat atttgtttac ggtagctttt
    10321 gaactaccac ctgcacacac gaacactcaa aagatgctaa ttttgattcg acagaatttt
    10381 ttacaacgtg tttgttcgaa acgtcttttc aaggttgatc atttattatc ttttcattca
    10441 ataaatatga ggttagcaat ttttatcaaa ccgccatagc cacatctcga ttcaaggata
    10501 aatttgtttt ctcatatctt atctctcatg acagtgcacg atatatatct tttccaaata
    10561 ttcatcactt ttttcctgtt aaaatcattt ctattattaa tgctttaatg acaacttatt
    10621 taatttattg catttaatgc cctgcttcat tttcaaatat taactttaat cgactagctc
    10681 atcacactat ttaccctttc cacctatcac tactgaattc aaaacgattt gtttacaaat
    10741 ccccttatgc caacggtcga atttcatcat gtttacattt aattgcaaat gccctcttga
    10801 aatattttaa aattaatatt attcttagca tactagtcgg aattatattc aaaaatttga
    10861 ggagtgacgt tcatgaatgc ttttcttaca ctgattaata ttattgtatt ggtgattttc
    10921 atcgtaatat tgcacatgat ggctcgcaaa catatatctt ttgcgaaacg tgtgtttaca
    10981 gctttaggga ttggtatagt attcggtgtg ttattgcatc ttatttatgg cacacattcg
    11041 aatgttatta catcaacaag tgactggttc aacattgtcg gacaaggtta cgttgcattg
    11101 ttgcaaatga ttgttatgcc tttaattttc atttctattg tagctgcatt cactaaaatt
    11161 caaatcggag aaaagtttgc taaaattggt agtctcattt ttatcttttt aattggcact
    11221 gtcaccattg ctgcgattgt tggtgttgtg tatgccttgg tgtttggttt agatgcatca
    11281 acaattaacc ttggtaacgc tgaacaagca cgtggaagcg aaattgctaa acaagcgaaa
    11341 gatttaacag cacatacgtt accacaacaa attttggaat tattgcctaa aaatccattc
    11401 ttagatttca ctggacaacg tgcaacttcc acgattgctg tcgtgatatt tgcttcgttt
    11461 ataggatttg cttatttacg cgtagcacgc aaacaacctg atcatggtga attgcttaag
    11521 cgtgcgattg acgctatcta ttcactagta atggcgattg tgacgttcgt cttacgttta
    11581 acgccatacg gtgtattagc aattatggcc aacacattat cgacaagtga ctttggcgcc
    11641 atttggactt taggaaaatt cttaatcgca tcgtacgctg cattaattac gatgtatatc
    11701 attcacttaa ttattttaag cctgttaggt attagcccaa ttcgctatgt taaaaagact
    11761 ttggaagtat taatctttgc attcacatca cgctcaagcg cgggtgcctt accattgaat
    11821 gtacaaacac aaacaagacg tctaggcgtg ccagaaggta ttgccaattt cgctgcgaca
    11881 tttggattgt cgattggaca aaatggttgt gcaggcattt accctgccat gcttgcaatt
    11941 atggtcgcac ccgtcgctaa tgtagaaatt gatttacaat ttattgtaac gttaatcgct
    12001 gtcgtgatta ttagttcatt cggcgttgct ggcgtcggtg gcggggctac atttgcatcc
    12061 atattggtgt tgtcaacgtt aaacttgcca gtcgctttag ccggcgtgct catctccgtt
    12121 gaaccattga ttgacatggg tagaacagca ttaaacgtga acgattcaat gctagctggt
    12181 acaggtaccg ccaagttaac taaacattgg gataaagata ctttcgattc aaatgataat
    12241 gctgcattaa cttcacacta attcattgca agaaataccc gccccctcta gctatactta
    12301 tcattttaag ctagaggggg gcgggttatt ttggtatctt gctatcgctg tattaagcct
    12361 gatttttgca attgttctaa catatctaat cttgctttgt ttcctaggaa catctcaatt
    12421 tgctgcgacc atgtttcttt gcgattcccg tttgtacgtt gatcatagta ctcgctgatt
    12481 gtctggtcgt aatctgccat ttgtgcatac tgtgtttcct tatcagcatg atacttatta
    12541 tgatggaaga catggtcaaa tggtaagcgt ggcttggctg caccattttc gtcatctgcg
    12601 ggttccccta ctgccatacc aaataccggg aagacatagt caggtaagtc taaaatttct
    12661 cgaacgcgtt caacatcatt tcttaatgat cctaaaaaga caatgccata ccccatatct
    12721 tcagcagtta ccgcaatatt ttcggcaact aatgctgcat cgattgcacc tactagcaaa
    12781 ccttccgttg aaccatatgc attttccata tcagtttcag catgttgatc aactaaatga
    12841 tgacgataat aatcaataac aaagacgaat aaatagccat tttctacaac ataaggttgt
    12901 ccagaaactt ctcgtaaatt ttctttaatc ttctcatcgt cgataccaat aattgagtat
    12961 gcttgcagga aacttgacgt cgaagcgctt tgtccagctt ctaccaattt cttaacaacg
    13021 tcttcactta aaggtttatt cttaaatttt ctaacagaat gatgcttttt cacaagatta
    13081 tatacatgtt ctgacacgtt acccactcct cgatacttta caattaattt aaaatcttca
    13141 tattgcaatt gtacacgtcc aaactttttt atagcaatta tttgcttata agttaataat
    13201 tgaacttttt aaaatattgt ctcatacttt aaaaatccag tttctcactc attaccttca
    13261 tccatctcga acaaatattc tcacaccatt atcaataagg ttgacttagt tgagaataca
    13321 aatcttcttt ttttacattt tttacattaa aatcaagata ttaaatatag acacaacctt
    13381 ttaaatcatc aactacaaaa tccattatca aagcacttta tattatcttt ctttatttta
    13441 aaagtaatca ttcactttac aaagttatcc agttgactat tctggatcaa aatgtaataa
    13501 tgctaactaa gagattagaa ttattataat taagcgcaat caattgatag ttctaattca
    13561 tacttaaatt cttaggagga agatatttat gtcattaatt aacaaagaaa tcttaccatt
    13621 tacagcgcaa gctttcgatc caaaaaaaga tcaatttaaa gaagttacac aagaagattt
    13681 aaaaggttct tggagcgtag tatgcttcta tcctgctgac ttctcattcg tttgtccaac
    13741 tgaattagaa gacttacaaa accaatatga agaattacaa aaattaggcg taaatgtatt
    13801 ctcagtatca actgatactc acttcgtaca caaagcatgg catgaccatt cagatgcaat
    13861 tagcaaaatc acttacacta tgattggtga cccatcacaa acaatcactc gtaattttga
    13921 tgtattagat gaagctactg gtttagctca acgtggtaca ttcattatcg acccagacgg
    13981 tgttgtacaa gcatctgaaa ttaacgctga cggaattggc cgtgacgcta gtacattagc
    14041 tcacaaaatc aaagcagctc aatatgttcg taaaaaccct ggcgaagtat gcccagctaa
    14101 atgggaagaa ggcgctaaaa cattgcaacc tggtttagat ttagtaggta aaatctaagg
    14161 aggcattaca accatgctta atgctgattt aaaacaacaa cttaaacaac tattagaact
    14221 aatggagggc aacgttgaat tcgttgccag ccttggttca gatgataaat ccaaagaact
    14281 taaagatttg ttgacagaaa ttactgatat gtcacctaga ctatctcttt ctgaaaaatc
    14341 tttaaaacgt acaccaagtt tctcagtcaa tcgtcctggc gaagaaacag gtgtaacatt
    14401 tgcaggtatt ccattaggtc acgagtttaa ctcacttgtt ttagcaattt tacaggttag
    14461 tggtcgtgca cctaaagaaa aacagtcaat cattgaccaa attaaaaaat tagaaggttc
    14521 attccatttt gaaacattca ttagtttaac gtgtcaaaaa tgtcctgatg tcgttcaagc
    14581 acttaactta atgagtgtga tcaaccctaa catcacgcat tctatgattg atggtgcagt
    14641 gttccgtgaa gaatctgaaa acatcatggc agtccctgct gtctttttaa atggcgaaga
    14701 atttggcaat ggtcgtatga caatccaaga tattctttcg aaactaggca gtacggcaga
    14761 tgcatctgag tttgaaaata aagaacctta tgatgtctta atcgttggtg gtggtcctgc
    14821 tagtggtagt gcagcgattt acacagcacg taaaggttta cgtactggta tagttgctga
    14881 tcgtatcggt ggccaagtta atgatactgc tggtattgag aacttcatta ctgttaaaga
    14941 aacaactggt tctgaatttt cttctaactt agcagcgcac attgatcaat atgacattga
    15001 tgcaatgaca ggtatacgtg ctacagatat cgaaaagact gacgaagcaa ttaaagttac
    15061 gttagaaaac ggtgctgtct tagaaagtaa aacagtcatt attgctactg gtgcaggttg
    15121 gcgtaagcta aacattccag gtgaagagca attgattaat aaaggtgttg cattctgccc
    15181 tcactgtgac ggacctctat ttgaaaataa agacgtagca gttatcggtg gcggtaactc
    15241 tggggttgaa gcagcaattg accttgctgg tatcgttaat catgttacat tattcgaatt
    15301 cgctagcgaa ttaaaagcag acaacgtgtt acaagatcgt ttacgttctt tatcaaatgt
    15361 tgatatcaaa acaaatgcca aaactactga agttgtcgga gaagaccatg ttacaggtat
    15421 acgttacgaa gacatgaaca ccggcgaaga acatctactt aacttagatg gtatctttgt
    15481 tcaaattggt ttacttccaa acacatcatg gttaaacgat gctgttgaat taaacgaacg
    15541 tggtgaaatt gtgattgatc gtaacaataa tacgaatgtt cctggaatat ttgctgctgg
    15601 cgatgtcaca gatcagaaga acaaacaaat tatcatttca atgggcgctg gtgcaaatgc
    15661 agcattaaat gcctttgact atattatcag aaactaaagt ttcattcatt tcactgacag
    15721 tatcttacat ttgttaagat gctgttattt ttttatcccg acataaaatc atgttatgat
    15781 aaacttaata tatacaacga gatgtgcgat aatgcttaaa gtatgtacct ctcgcaaaca
    15841 aatcaatatg aaaaatgata ttgagaaaga aaagtatgtg aacgaaatta ttatgctttt
    15901 acgtacgata cctacgtcta aattagtaca accaattatg cagttattta tagataaagc
    15961 tatccttaac aacgtccaaa atgatgcatt attgatagaa aaagcaactc atgcacgtaa
    16021 tcatttacgt ctatggtcat tgcaaggtca tcgtgacatt ccaaactaat tcaatgttac
    16081 tctattaaac atgaatacat attttttcaa ttaaagataa actaaggagt tatattacat
    16141 tatgaaaaag agattactac taagtacatt tttagcatcg acattaattc ttacaggttg
    16201 cgcgtccgat caatctgata acgaagatca tcatacaagt actggcatcc atgcacctaa
    16261 aagcgctaaa aaattagaaa caaaagatat ctttaactca gataagaaaa atagtgatat
    16321 tagtgatgca gagatgaagc aagctataga aaagtatttg tctgtaaata gcgatatact
    16381 tgataacaaa tatattatgc aacataaact agataaacaa attgacagtc aaacaaaagt
    16441 gactgaaaaa caagcagaaa cacttagcca cttatctaac ttagctgtta aaaacgattt
    16501 gcatttcaag aaatttgtca ctgaaaacaa tattcctaaa gaatataaga aacccgttga
    16561 attaatgatg aattacttca aagctttaaa tagtacaatc gccaatgttg atgaagatat
    16621 tgaaaagctt agctatcaac cgcaaaataa aatcaatgtt gtcgacgtgc caacaaaata
    16681 tgccggtgat gttaataaaa agcaacaaga taaaattaaa gatttcttaa aatctaaagg
    16741 tatcaagagt gatgttatcg ataaataact gaaagccatc attgcgaaat gtaacacatc
    16801 attacatgag ttttaattca tgacaattct ttatctttaa aagtgactaa tcaatttagc
    16861 taaagtcatc atttgggcat caagcagcat attactgttt gatgcctttt taattgcctt
    16921 aaattctcta tctatgccaa aattcccatt agttgacgca tttgactata tacctaacat
    16981 attccatact tatattgact ctataaacaa aggatgatca tgatgaaagt tcgaattact
    17041 tttattattt tagcaatttt atcgacgatt gtttgcttgt tcttagcagc aatgcatcca
    17101 acaggaccaa acacagtaac ttttgaacag ccatatttat tcaccctcaa catcattata
    17161 atggtattag tcgcattacc ttcacttgtt ttagctatat atgattatat gagttttaga
    17221 atttcttctg ctattttaca atttctaggg gctatctctt ggttcttttt atcattgata
    17281 ttatcgctca cacaatttac accttttaca ttagcgtcat ttataacttc aattattttg
    17341 ttcacaagca caattatcac attagccatt ggtggtaagt ctgttgaaaa gaatgattcc
    17401 ccttaaattc caaatgaaaa aaaggttctg aaggccgcta taaaacacag tttttcagaa
    17461 cctctatact tctattcaat gatatatggt ttgcaatttt ctacctttaa atccacagct
    17521 tctgcccttg aaactttgtt aaaataaacc atcaaacaac gaatgacaac ttgatgtgca
    17581 acaatgacaa tatcatcttt ttgtgtatct tcattgacaa catgattcat aaaatgttct
    17641 acgcgttgat atacatcttc ataactttct ccttcaggcg ctttttgtga aaaactatga
    17701 cgaaagtctt taaagtttgg atcattgaaa tatttatcat atttcggatt cgcactgatt
    17761 tcatctttat attcaccctc aaatacgcca agtgaacgtt ctcttaatag aggggtagtc
    17821 gttgatgcaa tgtcatatgg aaaaatatgt tcaaacgttt gctgtgttct taataagtct
    17881 gaaacatata catgtttaat ctgtttctct ttaaaataat cacataaatc gtctgcactt
    17941 tttgtgccag tatccgttaa cggcacatct aattgtccac aaaaataaga tcgaaaatgt
    18001 ttattatcat aattcgattt tgattcgcca tgtctaacta aataaatcgt cataatatta
    18061 ctccttacct tatgtatttc atatctacca taacactttg actactaatt cgatatcaat
    18121 cttaatattc tattctaaaa aaagaattaa ttcatatttt aaattaatga ctctaaaaat
    18181 agtattgtct ttaatcaagt gatgtgttac tatatgggtg tagttcttaa atgactacac
    18241 agtcttttat attcatgaac ataatactcc tggatggcgc aacccaacgt catccttttt
    18301 ttatttgtca aatactcgct tttttatttc caaaatttcc tcatattaat ttattacttt
    18361 ttaatcctat cttatctaca ttttcaaata ttataataca tattgaccat tttcacgcaa
    18421 ataaaaaaga tgacaactga atgccatctt tatttcaatt tccaattgaa tttttttatt
    18481 atttacgcat agctcttaaa attaacgtta cgattgcaat taagataatt gaaccaatta
    18541 atgctggcaa gatgtaaata cttcctaatt caggacccca ttgtccgaat agtttgccac
    18601 ctacccatga tccaataata cctgcgataa tattgcctaa aataccacct gggatatctt
    18661 tacccataat agcaccagca gcccatccaa ttaagccacc gacaattaac attccaataa
    18721 atccaaacat aattttcagt ctcctttttc tatttatttt gcgttattct aagtagtacc
    18781 ccttatttac aattctaaaa caaattcaaa ttatttttat ccaaatattt ttaaaagtag
    18841 taattgaata tcaattttat tcaatgtagc tatcgttatt taaagtctct gtaccgataa
    18901 tatcatatac atttacatta ttttttctgc cgaattcata gcttgattat tttatgttat
    18961 aggactagaa tatacacata ttattagagc atctttgaat tttaaatcaa gaagcgaggt
    19021 taatgaacaa tgaatatgca tattttatat aacttacgaa ctaaacataa tttagaaatt
    19081 gacgaattag cacagcaatt aaatgagaaa tatggtacta aatatgaagc acatcaaatt
    19141 tgggaatggg agaatcatca ccatgaacct aaatttaaag atgccatgca tttagctgac
    19201 ttctttgatg caccatatga aatgttttta gaaagtaagg ttaaagaata tcagaaacat
    19261 ttagaagaag tcgatattcg catggataaa tagatgcaaa taaaccctca caacacgttt
    19321 ggcatatatc ctttcaaatc tatactggat atattacatt acgttgtgag ggtattttat
    19381 taattaatat gaattaagac attttacaag cgttaatgca acgaatcttt ttagtgatct
    19441 tgctcactct ttaatacttt accgttctta gcatcaacag taacttcttg ttttttatta
    19501 ccttttttca aatcgatatt gtaaacaagt ttgccatcat ctttttcaag tgaccattct
    19561 ttaatatcac catcaaattc tttttgtcct tctttaatgg cttttttgta atctatagca
    19621 tcgctatatt taaagttatc attttcattc attgtatctt ctttttcagt ctttttgtta
    19681 atcacttttt tatttttatc agcaacaagt acttctgact cttcaccaga tttttgttgc
    19741 gtcactttat aagcccattc accattagaa ttttcaaatg aaattccttt caacttttgg
    19801 cctttgtaag tttcttcagc ttttttcaca gcatcttctg ggcttgtttt aacatctttt
    19861 aaagcaataa catcttttgt tgtattagtg tcttgattag tatttgactc tgttgatttt
    19921 gtttcatctt ttggagtatc attgccacat gcagtaagca ccaccgctga cattgataac
    19981 actgctaatg attttaattt cataatatca ctctctcttc ctatttttga aactcataac
    20041 aaaagcttat atgctatata gattgtatta ccccttgttt ttaattttat tcataattat
    20101 tacaaatatt tttaaattaa tcgtcatgcg ttactttcgt tcgtactcct tttataaatg
    20161 aaccatgtaa tataagcatg ctattatcga ctcagtttgt ctaatgcttt ctttggtact
    20221 tcttcctttt caacttcttc aaaagtttct acatgatgac ctttatgtgt aattttcaag
    20281 tatctatgcg gtttaacatc aaatgtagca gtatacatta attgcgtctc tttcccttct
    20341 ttattaaaaa cactttgact ataacttcgg agttgatcat ccattctagt agacacctct
    20401 gtcgttttaa cataagaatc atccttcttt actaatgggt taaattgatc tgttatacca
    20461 tgggagtcta ttgtttttaa aatgaataaa gcagcataag cgccaatgat agtaagtaca
    20521 agatatgtta ttgtttttaa aatcgttttc aaaagatcac ctctgacaaa atataaaata
    20581 ggatgtatct gaaaacaact tacataattg taattaaagt gtaagtatta ggagaaaatg
    20641 atggtatttg aatctgaaag atacgaatcg tttttaaact aagttatttt atagagactt
    20701 tattatgaaa ttgaaagaag ttaattttaa ggttgtcgaa tgtaaaaaca tattgattaa
    20761 ccataaaaga gaaattattt ttgtagattt cggcataaac gtcattaaag aattttctat
    20821 atgatggtgc taatcataac aaagaaagct aggcaccttt agatacctaa ctttcaaagt
    20881 attagcgata aaacactata tatactaatt tagcttaatt tcaaacttgt tcaatgtcat
    20941 taacactttt cacaagtttg aaagttacat tttttatatc ggctgtatta atttttacat
    21001 tttccctatc tttttgtagc tctttatgcg actctaatgt atatttacca ccatcttttg
    21061 tatttatgac gatgttacct ttaggcaaat gttctcccat atacaagcca taggaaatat
    21121 gagcaaatct tatgatatga tccaattctt ttaaagtgac aacttcttta ttaagagtaa
    21181 tattcctctt aggtgtatac actctgccag caactgtgta tgttccttct aaatgtatac
    21241 gaacttggtt ttcatttaaa ggtctctctg aataaaccca attcctagat ttattagatt
    21301 ttccatttat tgtatccttg ctgtatctat cgtaaagttt tttaaccaaa gctttttctg
    21361 aattatttgg tacttcttca actttttgta attgtttatc ttgtacactt gaggaatttt
    21421 gtgttgacgc gtctgctttt ggatatattc caaatgttcc cccatagatg atacctagcg
    21481 atagaataga ttttaatacg attgaatttt ttgagtcata ttttttgaac atatttaatt
    21541 acctccttga tgtaaagctt tatttgctac aattataaaa ataatagacg tgttcatgaa
    21601 ttaaattcat cttaactctt gattaacttt aatttgctac cactctgaat ttaataacta
    21661 taaatcgtct acacataatt ggacaaaatc taagagaata aaatttgtta atttaaaata
    21721 gcaagcaatt caaagttata tgtgtaatag ataaaataga tatccctata gtgatgcgtt
    21781 actagctaaa cataataaca cattagaaga taatgaagtt aaggagttac tggattgttt
    21841 cgactatgta attaagtata aaaatatcca acgacaaaac gtaattataa aatggtaaaa
    21901 gctatggtac agtttcaaat tgctaatgac atgcgtatcg gtgagctact tgcaataaag
    21961 agagtaaata taaactatga agataaaacg ctagatatcg acggtaaagt taattggata
    22021 actgaaaaaa gacgggagca ttcggagtaa aggagacaac tgaaagaagt aatagctata
    22081 aggccacagg gctcactacc caaagcatcg acttactaag aacacttatg cttggaatga
    22141 taagtttatt gatagagagt acatattcac aaatacgtct ggtagcccta tcgactcgaa
    22201 caaaattagc cacattatta aaggggggcg ctgatattag ttctattaag aaacctataa
    22261 cgacgcatac attacatcat tcgcatatat ctacacttgc tcaattagga attaacttaa
    22321 aagcaatgca agagcatgta ggtcattcag attataaaaa aaatctagag atatacacac
    22381 atgttactaa tcagatggcg aaagatatga tgaataaatt tgaacgattg gggagttaaa
    22441 attggaaaaa gatgatacac tagcagaaat taagcctatg ctcaattttg atgagcaaat
    22501 agcgaaatta aaacagatga atatattttt taatattatt gacaccgaaa aagcaaatga
    22561 aattcttaga aaaaataatt acttcttcaa actagcttat ttccgaaaaa atttcgaaaa
    22621 aaagaatggc ggctatttca tagaatttgc ttatttatca gatttagcaa ctatagatat
    22681 gaaattaaga tacacaatgt tgcatttaac tttagatatt gaacatagtt taaagtatct
    22741 agtcttaaaa ctaataacag aaaataacca agaagatggt tataaaataa tagatgagtt
    22801 cttatgtatt gataaatcat atagcaattc aaattttgac acaaattcaa gaacaccaga
    22861 agaagttatg gaaaccaaaa tcaaaaataa aaacgaaata ttcaagcata tgaataaacg
    22921 aggacaacta cccgagaagt tgaataaata ctatcaaaat ccacccgcat gggtttgcat
    22981 tgaattcatg caactaggtc aattcgtttc gtttctcaac ttctattaca agaagtacaa
    23041 tgacgaagaa ttgagagttg ctaatatttt aatgccttta gttaaaaata taagaaacaa
    23101 atcagctcat aaccaaccca tcatagcaaa tctaaattat gacagtagat tacctcaata
    23161 tttatttgaa aaagggaata atataggcat atctagaaac atgttcggaa taaaaaattt
    23221 catagatact ttcgctacgc tagaattaca taatcaagtt tgtagtaatg caattatcca
    23281 agcaagatat cacgatttgg accaacttca aaagcgatat aaaagaaacg aaagctatta
    23341 taataatgca ttagctatca aaagattttt tatagcttta gataaaatta ttgacttcaa
    23401 cagaccaaaa gtataaacta tctagtgagg aaagagactt ataggtctcg cgagttattt
    23461 taattcgtat gcaagaaaaa gaagagctat gcattttatt taaaatgcgt agttcttttt
    23521 ttatgcatct aaattcatat tatttttgca atataaacat atctttgtgc aaattccgaa
    23581 cacaaaacat tcacatcatc cttttttgcc ctttttctat accccaaaac acaaaaagcc
    23641 ccgtaagcct atgcctacgg ggtttgacaa taaattatat attattgttc ttctttaaca
    23701 tatggtaata atgccatatg acgagaacgt ttgatagctg tagtcaacat acgttgatat
    23761 ttagctgaag taccagttac acgacgtggt aaaattttac cgcgttctga gataaaacgt
    23821 tttaataatt cagtgtcttt gtagtcgata tgtgtaatac catttgctgt gaaatagcat
    23881 acttttttac gacgacgtcc gcctcttctt ggtccacctg ccatgattgt gtgcctcctt
    23941 tgataatttt ttcgctttaa tttcgttaat ttttattaga atggtaagtc atcatcactt
    24001 atatcaatcg gtccgtttgc atttgcaaat ggattatcag attgtttcgt gtttgatgaa
    24061 ttattgtacg aattgttttg tcctgattgt tgaccaccga atccttgacc gtaatcttgg
    24121 aattcatttt gttgacgttg gccaccattt tgttgcgcat ttttaggttc aaggaattga
    24181 acgctatcac acacaacttc agtaacaaac acacgacgac cttcttgatt ttcataatta
    24241 cgggattgta agcgaccatc tacaccagct aaactacctt tagataaata gttatttaca
    24301 ttatctgctt gtcttctaaa aacaacacag ttaataaaat ctgcttcgcg ctccccttga
    24361 gcattcgtga acgtacgatt tactgcaaga gtgaatgtcg ctacactcac acctgaggga
    24421 gtggttctgt attccggatc tttcgttaaa cgacctacta atacaactct atttagcatt
    24481 taaacgcccc ctctaattat tacttgtctt cgtcttcacg aataaccatg taacgaatga
    24541 tatcgtcact gattttagct agacgttgga attcgtcagt agctttgttg ttatcagatt
    24601 taacacgtac gatgttgtag aagccatctt tgaaatcatt gatttcataa gctaggcgac
    24661 gtttacccca gtcttttgct tctaaaactt ctgcaccttc agtagctaag ataccgttga
    24721 aacgttcaac taacgctttt ttagcatctt cctcaatgtt tgggcgtacg atgtacataa
    24781 cttcatatgt tctcatttta tatttgcacc tccttgtggt ctatacggct tatcaatctt
    24841 aaaacagata agcaaggaat aattttcatt actcacaata aagaattata tcatgcgcca
    24901 ttacttttta caataataat tcaaactact cttcatatca tttttgatat taattcattt
    24961 gaaactttct atgatatttt taaaaataca cttcacaaaa gcgaacatat gttctataat
    25021 agttgtgagg tggtaaggaa tgaatttaaa tattgattgg tctaaagatt ttcaagaatt
    25081 ccaagagata cttaatagtg gtattcatcc tgaatggctt tattgtgcaa aggctaatct
    25141 tgttttagag cctgcttata ctggcgaagg caaacaattt ttcagcactc aagatattat
    25201 taacgcgagt aaaattattc cattctttta ataaattata cgcataatta aaagaggcga
    25261 acaattgatt ccgcctcttc tacatatata ctatttaatt aacttcactc tatcgtttat
    25321 acattaaatc tgaaatgaac gatatcgcca tcttgcataa tatattcttt accttctaat
    25381 cgttgtctgc ccgcttcttt agcgccactt tcgccaccat attgtacata gtcgtcataa
    25441 cttgttactt cggcacggat aaatccacgt tcaaaatcag tatgaatgat accagcacat
    25501 tgaggtgcag tcataccttg tttaaatgtc caagcacgta cttcttgcac accagcagta
    25561 aaatatgttg ataatcctaa taattcataa gttgttctaa ttaatcgatc taatcctggt
    25621 tcttcgatac ctaaatcttc taagaacatt tctttatctt catcatctaa tgtagcaatt
    25681 tcttcttcaa tttttgcact aataacaatc acttcagagt cttcttgcgc tgcatattca
    25741 cgaatcgctt ttactttatc attatcatca tcaccaattt catcttcacc aacattagcg
    25801 atataaagca tttttttaga agtcagtaat tgcgcttgat tcacccattt ttgatcttct
    25861 tcattaaagt caatactacg agcgggttta ccattttcta aagcttcttt aatagttgtt
    25921 aaaatacgta cttccatttc agcagtctta tctttttgac gtgctaattt ttcaattcta
    25981 ggcaaacgtt tctcaacaga ttctaagtcc gctagtacta attccatatt aataacttca
    26041 atatcatcaa tagggtctac tcgaccagca acatgagtta cgttatcatc atcaaatgca
    26101 cgaacgacct gacaaatcgc atctacttct ctaatatgtg ataagaattt attacctaac
    26161 ccttctccct ttgaagcacc tttcacaata ccagcgatat ctgtaaattc aaatgtagtc
    26221 ggcaatgtct ttttaggttg aaccatttct tctaatttaa gtaatctagc atctggcact
    26281 tctactatcc ctacattagg atcaatcgta gcgaatggat agttcgctgc taaagcacct
    26341 gcttttgtta ttgcattaaa taatgttgat ttaccaacgt ttggcaatcc aacgatacct
    26401 gctgttaaag ccattaatca ttctcctatc tttgtgtatc atcatgagat tcgatgattt
    26461 ttttaagttt tttatcaaac gtttgacgtg gaatcataat acttctttga caattttcac
    26521 atttaattct tatgtctgca cccattctaa taatcttaaa acggtttgtt ccacacgcat
    26581 gttgtttttt catttctact atatcattta ttccatattt tgacgccatt aaatatgacc
    26641 tccatgtatt ataaactact aaacaccgtt ttcactatga ttataagcag tcataattgg
    26701 ttgaggtgtt ttaatacctt cttgtaaaaa cattttttgt acttctttgc gaataattcg
    26761 agctccagca aatccttcac ctggaattgt ttctgcagat attctcaata ttactcttgt
    26821 atcttcaata gcatcaatac caataacaac cggatcacta acaaataagt aatatttact
    26881 acgtaaagaa gtaaatagtt tgtttagttt tttatcaaca ttatcaatgt tttcttcgac
    26941 agacactgga attttaacga tagctgtacc atttgtaatt gagtagttcg ttatttcacc
    27001 catactacta tttggtaaaa ttgttaattc tcctgaaatt gtattgattc gtgttgaacg
    27061 aagtccaatt gatttaactg ttccctctgc tacagttgta ccaccgttat ttatcttaac
    27121 ataatcacct acatcaaatt ggctttcaaa tataataaag aatcctgtaa ttacgtcttt
    27181 aactatagtt tgagcaccaa aacctactgc taagcctacg acaccagcac tggcaattac
    27241 cccttcaaca ctaatgccaa atttacttaa aatcgtcgta ataactataa accaaacgat
    27301 atacttcact acattttgaa caagagatat taaagtttta gagcgctttt tgttactctt
    27361 tttactttta ttttgaatct taaatccctg ttcaatcatt ttattcagta ttttaataac
    27421 tatgagggct acgataatat aaataacaat catagcgatt ttagttgcaa tgttttcata
    27481 tgtttctatt tttgttaatg gctcaaatag agatgaaata atattcatga cttgattcat
    27541 gataagcgtt actcccttct atatgatata tcaatattgt attcatattt ttatatactt
    27601 ccaacctacg aaagtctcaa tatatctaga atcaaatgtt cactgattac aatttattta
    27661 cacttgcaca aagagttatt atataaaatt gtgtaactat ttaccatacc tacgatttaa
    27721 ttgttcaatt attctcacaa aatcttcttg tgaatcaaac tcaaatgaga ttttaccaac
    27781 cgattttttt attgatatat ctactttggt accatactgt tctcgcaact gtcgttcttg
    27841 ctgctttata aatttaggct tagttatatc tactttgtct gtttccgact ttgacgaaac
    27901 attttttaat tcattaacat ggttttctaa atatctgaca ctccactttt ctttaacaac
    27961 ccgtttcgct aacctaagca tttgttgttc atctttaatt gccaataacg ttcgtccatg
    28021 tgcacttgtc agtcgcccat cttttaccat gtcagcaatc tttttcggca aatgtaataa
    28081 cctcaacata ttcgctatat acgggcgcga cttactcaat cgtttcgcta cttcttgttg
    28141 tgtaattttc aaatctgtca tcaaacgttg ataactttca gcttcttcaa tcgcatttaa
    28201 gtcttctcgt tgtaaatttt cgatgaccgc cagttccatc atatcttcat ctgttaaatc
    28261 tttgataatc gctgatacgt attttagacc agcaattttc gaagctctaa accttctttc
    28321 accaactaca atgtaataac cttgaactgt ttttctaagc acaattggtt gcaaaattcc
    28381 atattgctta attgaatctg ccaaatcatt taaatgattt tcatcgaaag tttttcgggg
    28441 ctgatacgga ttcggtttga tatccgatat atctatttgc ttaatttgtt catcttttgt
    28501 tttagtaata cgttgatctt cactttttga caattcactc acatcacttt ttatttgata
    28561 acaacagtta aatatatttt atgtattcta aatgagtcag acaaccatta accacacttt
    28621 atttaaaaaa taattcattt ttcaacttca agataccaat tacaatttca caaacaaatt
    28681 aacttttaaa tcaaaatttt ctgaataatt gttgacaaat ctttttttac tctgtaagct
    28741 aattaatatc ttataacagt ttaatgaaac gtaaacacaa taaagaggaa agtaaaacac
    28801 accctgctta tacagagagt ctttagtagc tgagagaaga ttttgaaagc gtgtttgaaa
    28861 atggccttgg agtgttgatg ccaatatgag gtgtctacgg gttcgcccgt tatagcgata
    28921 cagtattaac attgatgtta aatggcgtac tggattcttt acgcacgatt ttttgttaat
    28981 aagtatggga tagcacatta ctatatcctt acttactgac tttaattgtg ataattgttc
    29041 agtaagcata tttactttta atgcgtactg aataaggtta tttcagcgat ggaataacaa
    29101 ataaaggtgg taccgcgaaa cataagcttt cgtccttttt atccgattca ttcgggtacg
    29161 aaggacggaa gcttttttta ttttttctta taggagggtc ttaatatgaa ggatacacag
    29221 ttagcccaaa tcacattaac cgatgattca accggtgcta tagcgaatcc aatccattta
    29281 tctactgcct acaagcatcc aaaactagga caatcgacag gttttgatta tacacgtact
    29341 aaaaatccta cacgctcaac atttgaaacc tgttttgcca aacttgagca tggtattgca
    29401 tcattcgcta catcaagtgg aatgtcagcc attcaattaa tatgtaatct atttaaacct
    29461 catgatgaaa ttttagtttc attcgattta tatggtggca catttagatt atttgaattt
    29521 tacgagcaac aatacgatat caaatttaag tacgttgatt ttacagatta tgaacaagtt
    29581 gaaaaagaaa tcactgataa aacagttgca ttattcattg aaccaatatc taacccacaa
    29641 atgattgcta ttgatgtaaa gccatactat caactttgta aagctaaagg cttattgtca
    29701 attatcgaca atactttttt aacaccttat ctttcaacac cactagcaga aggtgctgat
    29761 atagtcttac attcagccac gaaatatatt ggcggacata acgatgtact agcaggtgtc
    29821 gtaaccgtca aagatgaatc actcgcgcaa cagttgtttg attttcacaa catgactggc
    29881 gcaacacttt caccaataga tagttatttg ttgttacgtg gacttaaaac tttgcattta
    29941 cgcattgagc gtgcgcaatc aaacgctaga aaacttgcta aaaaatgtca gtcacttcaa
    30001 gcaattgacg aagtactata tagcgggcaa actggcatgc ttagtttaag acttaacaag
    30061 gcctatagcg tcgctaaatt attagaaaat ttagacattt gcatttttgc agaaagttta
    30121 ggaggtactg aaacattagt gaccttccct tacacccaaa cacatgttga tatgccagat
    30181 gctgaaaaag ataaacgtgg cattgatgag tatttaatcc gcttatcact aggtgttgaa
    30241 aattatgaag acatcgaacg cgatatcatc caagcattag ataaagctca gattggagag
    30301 attgtatgac actttcaaaa gagacagaag tgatattcga ttggcgtaga ggtgtggaat
    30361 atcattcagc taacccacca ctctatgatt cttcaacatt ccatcaaaca agtcttggtg
    30421 gcgatgttaa atatgattat gcacgaagtg gcaaccctaa ccgtgaactt ttagaagaga
    30481 agttagcacg attagaacag ggaaaattcg cttttgcttt tgcatcaggt attgctgcta
    30541 tttcagcagt acttttgact ttcaaatctg gtgatcatgt catcttaccc gatgatgtat
    30601 atggcggtac ttttcgcctc actgagcaaa ttttgaatcg atttaatatt gaatttacaa
    30661 ccgtagatac tacaaagctc gaacaaatcg agggtgccat tcaatcaaac acaaaattaa
    30721 tttatatcga aacaccatcg aatccctgtt ttaaaattac tgatatcaaa gctgtttcta
    30781 aaatagccga aaagcatgaa ctactggtag ctgtggacaa tacatttatg acaccgttag
    30841 gtcaatcacc tttattactt ggcgctgata ttgtcattca tagtgctaca aaatttttaa
    30901 gtggacatag cgatttaatt gctggtgctg tcataactaa taatgaggca attagtgacg
    30961 ctctttattt aatacaaaat ggtacaggca atatgttatc tgctcaggat agctggacac
    31021 ttgctaaaca tttaaaaact tttccaatca gatttaaaca atctgtcgaa aacgctcaaa
    31081 aaatcgtgtc atttttaata aagcaagatg aaatttcaga agtttattat ccgggactca
    31141 ctactgctca tttagaacaa gctaaaaatg gcggtgccgt tattggcttt cgtttagctg
    31201 atgagtctaa agcacaacaa tttgtcgatg cactgacatt accactcgtt tcagtgagtc
    31261 tgggcggtgt tgaaacgatc ctttcacatc cagcaacaat gtctcacgct gcactacctg
    31321 aagaagtgag acaagaacgt ggtatcactt tcggtctatt ccgattaagt gttggtctcg
    31381 aagatcctga tgaactcatt gcagacatca aatacgcatt aaaggaggca ttcaatgagt
    31441 caattcctca cacaattgaa agataatgtt ttagtagctg atggcgctat aggaaccatt
    31501 ttatactctg aaggattaga cacctgtcca gaagcatata atcttagcca tccagataaa
    31561 gttgaacgca tccatcgttc atatattgaa gccggtgctg atgtcattca aaccaatact
    31621 tatggtgcaa attttgaaaa gttaaaacga ttcggtcttg aagataaagt taaagcaata
    31681 catcaagccg ccgttcgcat cgcaaaaaaa gcagcaaata aagatacgta tatattaggc
    31741 acagttggtg ggtttagagg tatcaaacaa gaggatatca gcttacaaac tattctttat
    31801 catactgaaa ttcaaataga caccttaatt gaagaaggcg ttgacgcgct acttttcgaa
    31861 acgtattacg acctagaaga gttaacaaat gtcatttcac gaacgagaaa gaaatacgac
    31921 attccaatca ttgctcaatt aaccgcttca aacacaaatt acttagttaa tggtcaggca
    31981 atcaatgaag gattaaaaca actcgttcaa tgtggtgcaa acatcgtggg actcaattgt
    32041 catcatggcc cgcaccatat gcaagagtct ttcacacata ttgaattacc agagcacgca
    32101 ttcttatctt gttatccaaa tgccagctta ttagatattg aaaatagtga atttaagtat
    32161 agtgacaatg cacaatattt cggtcaagtt gctcaaaatc taattcgcga aggtgttcgt
    32221 ttaattggtg gttgctgtgg tacaacgcca gagcacatca aatttattaa agaatctatt
    32281 cagacactta aacctgttaa tgacaaaaaa gtgattccga taccaacgaa agcacttttc
    32341 aatccatctc aaaataaagt tagacaatca ttaacatcta aggttcaaga acgtccaacc
    32401 gttattatcg aattggatac accgaaacat ttagacacgg atagattttt tgaaaatatc
    32461 gctaaacttg ataaagctaa tgtagatgcg gtaacactcg cagataattc attggcaact
    32521 gtcagaatta gcaatattgc tgctgctagc ttaattaagc aatattacaa tattgaacca
    32581 ctcgtacata ttacatgtcg agaccgaaac ttaatcggct tgcagtccca tttacttgga
    32641 ttatcgctca ttggcgttaa cgaaatatta gccataactg gtgatccttc aaaagttggt
    32701 cacttaccag gtgcaaccaa tgtctatgat gttaattcta aaggattaac tgaactcgct
    32761 ctaagattta atcaaggtat taacactgac ggtgatgcgc tgaagaaacg tacacacttc
    32821 aacatcgctg gcgcctttaa ccctaatgtt cgtaaattat atggtgccgt caaaagatta
    32881 gagaaaaaga tagaaagcgg aatgtcttat tttataacac aacccgtgta cagcaaagag
    32941 aaaatcattg aaatttacca tgccactaag cacttgaaca aaccattttt cataggcatt
    33001 atgcctatcg caagttacaa aaacgcactc tttttgcata atgaagtgcc aggaatcaag
    33061 atgtcagatg aaattttaca acaatttgaa gcagttaaag atgataaagc caaaacacga
    33121 gaactaagtc ttaagctttc aaaggattta atcgatactg ttcatgaata ttttaatggt
    33181 ttatacatta tcacaccgtt tcaaaatgtc gaagattcat tagaacttgc agcatactca
    33241 aaatctatta ctgctcacaa ggaggcaata ttatgacaac aattaaaaca tcaaacttag
    33301 gattcccaag attaggtaga aaaagagaat ggaaaaaagc catcgaaagt tattgggcaa
    33361 agaagatttc taaagaagaa ttagatcaaa cattaactga tctacataaa gaaaatttat
    33421 tattacaaaa gtactatcac ttagatagca tcccagttgg tgatttctct ttatatgacc
    33481 atatattaga tacgtcatta ttattcaaca tcatccctga acgtttccaa ggaagaacta
    33541 ttgatgatga tttattattt gatattgctc gcggtaataa agaccatgtt gcaagtgcac
    33601 ttataaaatg gtttaacact aactatcatt acatcgtacc tgaatgggac aatgttgagc
    33661 ctaaagtgag tcgcaatgta ttattagatc gctttaaata tgcccaatct ttaaacgtta
    33721 acgctcaccc tgtcattgtt ggtccgatta cttttgttaa attatcaaaa ggcggtcacc
    33781 aaacttttga agaaaaagtt aaaacgttat taccacttta taaagaagtg tttgaatcat
    33841 taattgatgc aggtgctgag tacattcaag ttgatgagcc aatcttagtt acagacgaca
    33901 gcgaaagcta tgaaaatatt acacgtgaag cttatgacta tttcgaaaaa gctggtgttg
    33961 ctaaaaaatt agtcattcaa acatactttg aacgtgcaca tcttaaattc ttaagttctt
    34021 tacctgttgg tggtataggt ttagactttg tccatgataa tggctataac cttaaacaaa
    34081 ttgaagctgg agattttgat aaatcaaaaa cattatacgc tggaattatt gatggtcgta
    34141 atgtatgggc aagtgacatt gaagctaaaa aagtcttaat cgataaattg ttagcacaca
    34201 ctaatgaact tgtcattcaa ccatcatctt cattattaca tgttccagta tctttagatg
    34261 atgaaacatt agatacaagt gttggcgaag gcttaagctt tgcaactgaa aaattagacg
    34321 aattagatgc attgcgtcgc ctatttaatc aaaacgatag tgttaaatat gataaattaa
    34381 aagcacgtta tgagcgtttc caaaatcaat cattcaaaaa cttagattat gatttcgaaa
    34441 gcgtgcgtac ttcaagacaa tcaccattcg cgcaacgtat tgaacaacaa caaaaacgtt
    34501 tgaacttacc ggatttacca acaacaacta ttggatcatt cccacaaagc cgagaagttc
    34561 gaaaataccg tgcagattgg aagaacaaac gcattacaga cgaagcatat gaaacattct
    34621 taaaaaatga aattgctcga tggattaaaa ttcaagaaga cattggctta gatgtattag
    34681 ttcacggtga atttgaacgt aatgacatgg ttgaattctt cggagaaaaa ttacaaggtt
    34741 tcttagtaac taaattcggt tgggtgcaat catatggttc acgtgccgta aaaccaccaa
    34801 tcatttatgg tgatgtaaaa tggacagcgc ctttaactgt tgatgaaaca gtttatgcac
    34861 aaagcttaac agataaacca gttaaaggta tgttaactgg acctgtaaca attctaaact
    34921 ggtcatttga acgtgttgat ttaccacgta aagtcgttca agatcaaatt gctttagcaa
    34981 tcaacgaaga agtattagca cttgaagctg caggaatcaa agttatccaa gttgacgaac
    35041 ctgcattacg tgaaggctta ccattacgct ctgaatatca cgaacaatat cttaaagatg
    35101 ctgttttatc atttaaactt gcaacgtctt cagttcgtga tgaaactcaa atccatacac
    35161 atatgtgtta ttctcaattc ggtcaaatca ttcatgctat tcatgactta gatgctgatg
    35221 ttatttcaat tgaaacatct cgtagccatg gtgatttaat taaagacttt gaagatatta
    35281 attatgattt aggtattggt ctaggtgtat atgatattca tagtccacgt attccaacaa
    35341 aagaagaaat tactacagca atcaatcgtt cactacaaca aatcgaccgc tcattattct
    35401 gggtaaaccc tgactgtggt ttaaaaacgc gaaaagaaga agaagttaaa gatgcattga
    35461 ctgtgcttgt gaatgctgtt aaagctaaac gccaagaata atcgcagcat ttatcatata
    35521 ataaactaaa aacgggtgat tttatgacac aatatccttt atggcaacaa ttaaatactt
    35581 taaaacaagc aacttgggtt gatttaacgc atacatttga tccggaaata ccacgtttta
    35641 gtgagtttga aaaaggcgaa gtttcaactt tattcacggt taaagatcat ggtttctatg
    35701 tgcaacgttg gaatattgta acgcaatatg gtactcacat tgatgcacca attcactttg
    35761 tcgagaataa acgttattta gaagacattg atttaaaaga attggtattg ccgttaattg
    35821 tattagactt ttcaacagaa gtagcgaaca acaatgattt catcgtaaca cgtgcgcata
    35881 tagaagcatg ggaaaaagag catggtacta ttgaacctgg cacttttgtt gcacttcgta
    35941 ccgattggtc aaaacgttgg cctaatattg aaaagtttga aaacaaagat gcgaacggac
    36001 aacaacatgc accgggttgg ggattagatg ctttgaagta cttaattgaa gaacgtcgtg
    36061 ttgaagctgt tggtcatgaa acattcgata ccgatgcatc tgtggacgtt gtaaaaaatg
    36121 gtgatttagt tggcgaacgt tatattcttg gtcaggataa ataccaagtc gagttattaa
    36181 ctaatttaga tcaattacca actcgtggcg ccatcattta cgctatcagc cctaaaccaa
    36241 aagatgcacc tgggttccct gttagagcat ttgcaattaa accatcaaat gactaatatt
    36301 acccaataaa tccataaaca aataaaaaca ggttatgcca acatgtaatt aatactgttg
    36361 tgataacctg ttttcttatt atttatactt tgatacaact gcagcgatag cttgaccgcc
    36421 accaatacac aatgatgtta aaccagtttc aacttcatca ttcagttgat gcaataatgt
    36481 cactaatact ctagcaccag atgcaccaat aggatgtcct aatgcaatag cgccaccttt
    36541 aacattcacc ttttcaggag gtaattttaa ttcacgatca acagctaatg attgtgctgc
    36601 aaatgcttca tttaattcaa atacatcaat atcgcttaat tcttttttac tacgtttcaa
    36661 agccttttct acagcgccaa ctggtgcaat acccataata gaaggatcta caccatgact
    36721 tccaaagcca tcaagcactg ccaatggttc gatatttaat tctttagctt tgtcttctga
    36781 catgactaac atcatcgcag caccatcatt gattcctgat gcattacctg ctgtaactgt
    36841 accgtctttt ttgaaagctg gtcttaatcg acttaatttt tcgactgata cattttcacg
    36901 tacaccttca tccttagtga ctacgattgg ttcaccttta cgttgaggaa tcgatactgg
    36961 aactatttca ctatcaaatt caccattttg ctgtgcacgt actgcttttt gttgtgagtt
    37021 tacagcaaat gtatcttgtt cttctcttga aataccatat tgctctacta aattttcagc
    37081 agtaataccc atatgatatt gattaaatac atctgttaaa ccatcatata ccatgctatc
    37141 aaccattgat tgatgtccca ttttaaaacc aaagcgactg ttgttgacaa gcattggtga
    37201 ttgagacata ttctccatac cgccagctag cacgatgtca ttttcaccag tcacaataga
    37261 ttgatatgct aattgaatcg actttaaccc agaaccacat actttattca ccgtaaatgc
    37321 aggtactgtt tctggcaagc cacctttcat agcagcaatt cgtgctggat tttgtccttg
    37381 tcctgcttgt agtacgttac cgatgataac ttcatcaatc tcacttggat tcaaacccgt
    37441 ctctttaata atatgttcta ttaaagtcgc acctaaatca taggctggca cgtctttaaa
    37501 cgcacctcca aaaacgccaa taggtgtcct gtatgctgct gctaatacga ctctcgtcat
    37561 gtcatattca tctccttatg atatatacaa ttttattact aatacgtcta atgaaccaat
    37621 acgccactaa catccaattt atgaaaccaa ttgctacgta tgttacattt tcattcttgt
    37681 tatgcattta acaaatcata ttatacaatg atgtaatttg tttgggcaat cggaaacgta
    37741 aacggtttca ctttaacatt tcaccctctt aaatatgctc tatcatcctc atctatagta
    37801 caaaattatg ctttgattta ccacaaatta tcattaatca tttcgcgtaa tgactctaat
    37861 taaattctat ctatcattgc atcgctattc acagctggtt tcaccatcaa tttctcatca
    37921 attttttatt accatgacgt ctccagtcaa atattaaatc gctgatgatt gttaattccg
    37981 caattacctt gcagtcacct ctccttccac ttcatcgcca aatctgcccc tattcactag
    38041 aaaaaataaa tagtatactt agtaaattaa ttacacatct taaaggagtt aaaattttat
    38101 ggttaatgca ttaacaccaa aacatttagc tgaaaagcgt gcaggattcc atgagttgtt
    38161 ctttgattta attttcgtct atgccattca aaaaattgca catgtgattt taacaacaca
    38221 aaacggttcc atttcagctg atttattttt caaatacatc gtcatgagtt tgttcttatg
    38281 gttaatgtgg tctcatcaaa cattttttac aaatcgattt ggtcaagtta cattcaaaga
    38341 cgtctcgttt atgatgttta atatgttcat catggtcttc ctatctaaca gcctatatcc
    38401 ggactttgaa aagacattct tcccattctt cttatgcgtg gcaattatgt atttaagtat
    38461 cggattacag tatttattac atatacgtac agggttagat tatggcgata agcgtacatg
    38521 tcaagctttt gcgagcgtag cctttgtgat ttcattttta tcatttctat cattagtact
    38581 accacaaagt atccattaca tccctggatt tttaggcgta ttcattgcag cgacaggact
    38641 tattccattt cagaaatatc ttgtattatc acctgttaat atgatgcatt tagtagaacg
    38701 tttttcgtta ctaacaatta ttatctttgg cgaggtactg gttggtttag cttcgtcatc
    38761 atttagcatt gatcatttta gttacattta tatattccaa tttatgattt taattagtct
    38821 tttcggtgta tattggatca ttactgaaaa ttatattaat cataaattaa catctatcgg
    38881 tttccgttta tcgtatacgc atctattaat caatatcgct ttaggtgtga ttaatgctgc
    38941 aattgttttt agtaataaca ataaactaaa tgacttgttt gaaattaata tgatgtatat
    39001 cagtgtttta atattctata ttggattatg gttaatcacg ccttatttcc ataatgaact
    39061 gacaaacgcg aagtatattt catattcact aggtatactc gttatgtctt atattgtcag
    39121 ccttttattt aaaggtcacg accatgtaat gattatctca gtatcagttg caaccttctg
    39181 catcatgctg atttatttta aaaatcaacg tctacgtaat aaacatgaat aaatagtatt
    39241 tacctcggtc aaagtattgg aactgaatcc aaaagtgtcg aacttcacat tatacttttg
    39301 tgacaaactt cagttatgct acatgatcga ggtttttaaa tatttccgag gaaatcgtta
    39361 gcctatacaa tcatcaaatg atacttataa ataacatata tcctctaatc cccactatct
    39421 aaaaggaaat aattcccaca caacataacc acctcaaaac aaataaatca atatagatat
    39481 gtcacctata cttatccccc acatttcatt ccacaccaaa aagaacagat gacaatcgta
    39541 aagatttatc acctgctcta aatattattt caaatactat catcaccaaa atcgacaatt
    39601 atagttcttg tttgttaaaa attttaatag acaatatata agaaacgctc gttataacta
    39661 taccaatgac cgcatatgct atgacaacta gccatccttc agtgctaatt ttttcaaatt
    39721 ctaatgcaga tgcaccacta accataccat atataaagaa aaagaccacg aaagaaataa
    39781 gtagtagtat agatgcacat atcataatga cattggagtt ttcagcgcca aatttaaatg
    39841 tcaatgggaa aatgatagag tatgccccta tgataccaaa actcattaaa cctgacataa
    39901 taccaatcat cacactttgg gtcacaatcg tagtcacaac taatccaatc attaaacttg
    39961 caccgaataa gattaaataa aaggcaaagt atgacttaat ataatcacta cgtttaaccg
    40021 gtaaagtaga tacataatac atccatcttg agtctttttc atgtttaata ttatcagtaa
    40081 taggtgtgat taacataacc ccagccattg ccgaactcat caacggatta aatactgcaa
    40141 agtatcctgc agctataata gcgactataa aataaatata tgtttgcttt ctcgttgcat
    40201 aaaaactact taggaacata cctttcatta tacttcacct cgcattatga tttttgtagc
    40261 gtcatcaatg tgttttaaag gctctgcatt ttcaatttct gcataatctt taactaaaat
    40321 ttgatatttc ccctttgaca atctagaagc aatgattaaa tgcttaggaa tttcaacatc
    40381 tttatcttct gtcgtaacaa ttccatattg tgctaaaagt atatctttct gttcagttaa
    40441 aatcattcgt ccatctttca taaacactaa tttatcagct aaatgttcta tatcttcaga
    40501 aatatgcgat gatattaaga tgcctccacc ttgtgcgaca aaatcttcta gtatttccat
    40561 tacttcttct cttccagaaa catccatacc tgcagttgct tcatctaaga ttaataactt
    40621 cacatcatga gaaagcgcaa tcgttaaagc tatcttcatt cgcatccctc ttgaaaaagt
    40681 tttaatttta gtttgtagtg gtaactcgaa atatttgatt aaatcaaaga atttttgact
    40741 attccaagtc atataaatag attgaaatac tttatcaata tctttaatag tcaatttatt
    40801 aggcactctc aaatcatcaa acactacacc tatgtgttct ttatattcga catcatccac
    40861 ggtatgttct tctccaaaaa atgtgatact accgttatct ttatgtcgat tgcctactag
    40921 aatattaatt aatgtcgatt tacctgagcc atttttccca attaatccaa ctgtctcatt
    40981 agtagaaatc gttaaagatg catcttgcaa tttaaaatcc gaatctttgt attgtttgtt
    41041 cacactatct acgtttaaca atgttgtcat atccatgctc ctctttgttt aattttaata
    41101 aaaacgtctt ctcttcaata agtaactaaa ggcgttatat atgaaaatag caatgataag
    41161 tagcaaacta aagctttgat taattccagt agtaattgaa taaagcccaa ttactacaat
    41221 ggctagtatc aatattgata agttgatcgc atatgtttta aataatgcaa ttaattctat
    41281 atggcgttca ccttcatcca atatttccaa tcgcttttca gtgtaattct tatctgcaat
    41341 ttttggtatt ctgtcatcaa accttctatt aaacagtgta aattgtgtat tgaaaatagc
    41401 actagcaaaa aatggtatta aaaagaatag tattgcattt gctgcagcgt gccctaccac
    41461 aaaaataagc aatactaaaa aagcaataac tgtctgcaga atacttaata tacttccatt
    41521 taaaacataa cgattcgcaa gcaactcata ttgatctgtt gcatcgatat ctacctcttt
    41581 atctacaagt tgcttatact tcaatgcacg tctttgattc atcaaaacaa tggcttctac
    41641 caatatgata ataatcgtcg caacaatcga tattacaacg acattgttat atgtcgcaaa
    41701 cgtcaagttc tcaataccaa atccatcaaa attacctaaa attccaccta tgataccccc
    41761 tacaagtcca cccagtaaaa gatatccgat atatcttagt attttcatgc ctcatcctcc
    41821 tcaataataa aaacagtttc caccgtttca ttgaaaatgc gagcaatttt tattgccgtt
    41881 aatactgatg gcataaaatt gtttcgctca attagcgata tggtttgtct tgaaacgccc
    41941 gcttgtttag caagttgcgt ttggtttaag ccatctcgtg ctcgtaattc tttcaatcga
    42001 ttacgcacat cgcatcaact ccttaattac actgtaaatg atatgcgtct ttttgacaac
    42061 tatatttgtc aaatctacac caaaaaatat gattatccac ctatgtatga cattttgaaa
    42121 caaacacctc aacgcctaca agtcataatt gtttactttc gttacacctt cctgcataat
    42181 taacagcatt ctaattttag tatgatgcac gcattttcac taaatcaaac cattcaaagg
    42241 agactattat ggcatttaca ttatctgcaa ttcaacaagc acatcaacaa tttactggtg
    42301 ttgactttcc aaaactattc aaagctttta aagatatggg gatgacttac aatatcgtca
    42361 acattcaaga tggcactgca acatacgtac atcaatcaga agatgatatc gttacgtcat
    42421 ctgtaaaaag taatcatcct gttgctcaaa aatcaaacaa aacaatagtt caagacgtct
    42481 taactagaca tcaacaaggg caaacagatt ttgaaacatt ttgtgatgaa atggctgaag
    42541 ctggcattta taaatggcat atcgatattc aagcgggcac ttgtacttat atcgacttgc
    42601 aagaccaagc tgttatttca gaattaatcc ctcaataaac tatatttata gcaacatttt
    42661 aattatttca taaaatttta ttgataatca ttatcgttcg gtataaagta aatactatat
    42721 actacttatg agtgaggttg attatcatga taactaacac ttttatttta ggcatcacag
    42781 gcccaacaag tcttgtcgtc attagcatta tcgctttaat tatttttggt ccgaaaaaat
    42841 taccacaatt tggccgtgcc atcggttcta ctttaaaaga atttaaatct gcaacagaag
    42901 atttagataa agagtctcac gatacaccca gtaaggaatc gaaacaacag cgagagcaat
    42961 agcactgacc acaccttact ggttcacttt agcgaactac gccatcggtt agtaaaaatt
    43021 ttattgtcgt tcgtcattac ggtcatcgtc gtatatgttt catcattttg gtggatgaca
    43081 ccattcataa cgtatattac ccgtgcacat gtgtccttac atgcattttc attcacagaa
    43141 atgatacaaa tatacgtgat gattatattt ttcatcgcgt tttgtttcat ttcaccagtc
    43201 atgttttatc aattgtgggc attcattgca cctggattac ataataatga acgtcaattt
    43261 atttataaat atagtttttt cagcgtgtta ttattctgtg ctggcgtcgc ttttgcattt
    43321 tatgttggct ttccaatgat cattcaattc gcgttaaaat tatcattaac tttaaacatt
    43381 tcgcctgtca ttggctttaa agcatatttg attgaactca tacgttggtt attcacattt
    43441 ggtcttctat ttcaattacc aatattattt atgggtcttg caaaattcgg tcttatagat
    43501 accacatcat tgaaacatta tcgtaaatat atttatttcg cttgctttgt attagcaagt
    43561 atcattgcac cacccgattt aacattaaat atattactca cattgccact catattgctc
    43621 tttgagttca gtatgttcat cgttaagttc acatgccgag gcaaaccgcc aacacattaa
    43681 ccttatacga atcaatgctg tgattatcgt tatactgcaa taccgaatat ttccaatata
    43741 gcaacaaaaa aacaggcact cggcctgttt tttatttttt aaatttaaag attaagatag
    43801 caactaccat aatataagca ccttgtgcta tcaatggttc ataacttgga taaaagccca
    43861 accagttaat cgttgggaat ccttcaataa catgtcttgg catcgcacct aataattgta
    43921 acttttgaat acttacgcca agcattttga atcccataat aaaaataaag atcgacaaca
    43981 ctctaaatat atagaaaata ggtattaatt taactataaa tctaaataat aatgcaaaga
    44041 tgattaaaat aacgatagct aaagcaatac caataataaa atctttggtc gctagctcac
    44101 ctatcatccc catatagaaa atgataacct cgacaccttc acgcaacaca gatattaaac
    44161 caatcgtcgc taacaatacc aaattaccat tactaatcgc attagcatac atatttttaa
    44221 tcatgtcatt ccaacgtttt gcatttgaac gtttgtgcat ccaaacacca acgataaaca
    44281 ttaatatgac cgcaacgata cctaatcccg cttccatact ttcacgaaga atgccactat
    44341 tccctaaagt ttctacaaac gtaattgcta agataatact cagtacaagt ccggcaattg
    44401 caccaccaat cacacttgca gtccctttct tatcttttac attacgcgtc atggtagtca
    44461 atgtcattac aattaacaac acttctagcc cttcacgtaa aaagataatc atcacatcga
    44521 cgaagctata actatggcca acaacctctt taatttggtt atttaaatct actaaaccat
    44581 ctttcacatg tgctttatta tgttcgtcta atacactttg ataatatggt attttatctt
    44641 caattttcgt atacaaagca ccgtctttag tttgaatttg accttcaaca tacggccaag
    44701 tttctataaa atgtgtaagc gcagcatcag catccgacaa ttgattgtcg tcgatagctt
    44761 taatcgcctt ctctaacgca tcatttaatt gtgatacatg gtattgatca tttgcagacg
    44821 tattactttt tttatcgaca tgatcaatat ttgatttaaa agttgtccaa gcatgtgaca
    44881 cttttgccgt atctaatggt gacttatgaa ttgcaattct aagttgtaat aatgcgactt
    44941 caatttgtcc atattgattt gcgtcataat tgcgaatcac tgtttcatta cttgtccaaa
    45001 tctgattcaa actattgttc aaagattcta attccgcttt atttttatct ttaatcgctt
    45061 ttgtcatcgc agcatcttta gcatcgactt gctgttgcaa tagtttaatt ttagaacccg
    45121 catctttact agccaatttc tcttcataag caattaatga cttcgttaat tgcgaaagtg
    45181 tatctttttg attatcattc gcttttgcat cttcaagctt tctcacatct gatttgacag
    45241 cattactttc actattatct tcaagcgata atttcttaac tgcacttacc acttgctcaa
    45301 ttgctttctg cttattgtca ttcgatatcg aattattaga aagtgcagat ttcgcatccg
    45361 ttatcacact atatacatca ctaatacttt gttgttctgc tgcctgactt ttcagtaacc
    45421 caaagctaca caccatagca gcagttatta gcattgctac aaatttagtc aaataatgtt
    45481 tcaccaaggt atcctccctt actaacacct ggtaatacta aaaatgaagc agaacctcta
    45541 tgtgtaatat attcatttaa tttatcatta ctacctaaat tattttgtat atcgataaat
    45601 tgttttgtcg ctttttgaaa agcaataaaa agtaagcctg tttcgaagtt acctgtgcgg
    45661 tcatccgtac catccacata attaaaggct ctacgtaaaa ttgacgtatt tgcttctttc
    45721 gctagcctcg tatgggcatc tttatcaata atatactcgc catgactatc tttcgctttt
    45781 aagtcaattt catcaaactc tttcccacct gttaacggtg caccactatg tcgtttccga
    45841 ccaaatgtag cctcttgttc ttccagcgca gtacgatccc acgtttcaat gtgtatttga
    45901 atacgtctga caacgcaata agttccatgt ttcgcccatc cgtcatcaat aaacacataa
    45961 tctttaagtt gattattctt cctaggatta attgttccat ctttaaatgc cattaaattt
    46021 ctaggtgttt ccttaccttt agcagagata aaaccagatt gcgcccaacg taccttaaca
    46081 atatctcgaa atggacgaac taaattatga accgcatgaa aggatacttg cgaatcattt
    46141 gagcatgctt gaatcataat atcaccatcg ctgtaatcgt ctattaactg atcattcgga
    46201 aaatgcggta aatctttaaa ggcatcggga attttactag ataacccaat tttcttcatc
    46261 aaagacttac taatcccaaa ggtaatcgtt aacttgcttg cacctaatcc tatcgattca
    46321 ccggtatcta ctggtggcat taaaggattg ttactcggct tgccaactgt atcaccatcc
    46381 atcatacgat cagccataac cgtccacttt ttaaacattg ccttaattgc atctctatct
    46441 tttgacttca aatctaacgc aacgaaattg catgtttttt gcgtgggtgt ggtaatgcct
    46501 ggttgcactt taccataaaa ttcatacgca tctttttccg gatcttctgg tgtattgaac
    46561 attgatttga aagaccacat gctaccaaca ccacttgcgc caattgcaac accggcaccg
    46621 ccaataccta acattttcaa aaatgtacgt cttgaaaatt gcgtactatc gttaacttgt
    46681 tcataatttg tcattcagta atcacagcca ttttacttag tgactctcct aatgcattaa
    46741 cagcatccgc aaatgcttta cggtctttct tcgatacttt ttcaaatgac tcataaccgc
    46801 cgttgttatc tttatattta tccaataatt gattcacttt atcgaagttc atttggatat
    46861 catcacttaa ttttttatct tttttctcta aaataggttt aaataagtca taaatttttt
    46921 gtgcgccttc aacgttcgct ttaaaatcat ataaatctgt atgtgaataa atttcttctt
    46981 cacctgtgat tttagaagtt gcaacttcat ttaataggtc aacagaacct tgtaacatta
    47041 attttggtat gatatctaat gtatcagctt tggcatgcaa ttcttttgca tctttcaata
    47101 attgttgtgc atctttttta gtcacatcat caattttctt atcttcgtat aatgcttttt
    47161 caatcttatg atatcctgac cattcttttt cctttttctc ttctttcata tctgcaagac
    47221 gtgcatcaat tttaggatct aaatctccaa atgcttctgc aactggttca gagcgttcat
    47281 aatacatacg aacttttgga tataacgctt ttgcttgggc catatcatta ttttcaatcg
    47341 ctttaacaaa tttttctgta ccttttaaaa attcatttaa ctgttcatca gtatatttat
    47401 catatgcttt tgttgcttgt tttaattctg ctttaacacc atcatctttt ttcgatgtct
    47461 ttgaatcatc cttcttacta tcgtcgttcc cacatgcagc aattaataac gttgatgcta
    47521 ataatagcgt tgttaacttt ttcattttga cacctcatta tagaaattct attatcccca
    47581 tagatgtgtt cttataaata ttattttcgc attccctaca cgctaaaata atacattaca
    47641 cttaaaacgg ctgttttaaa gcatcctccc ataaacatca tctagttgat aataattatc
    47701 aaaatcatta tattgttata aaataatact gtcaacaacc ttttattttt aaaaattaaa
    47761 taaaagagcg ctgccatttt aaatgtcatc actcttttgt aagtctaatt tgtcattttt
    47821 ggtcgtattc tgattttaat aaactataga tgtaactcga tgaataaata ccatttagta
    47881 attcattgtc acgtaacata ccttctctag taaatcccag cctttcagga atagcccggc
    47941 ttttttcatt attaactgcg gcactaatct caattcggtt taagtcaagc tcttcgaaac
    48001 aatactttat caatgcctca actgcttgtg tcataatccc atgaccctca aattctttat
    48061 ctaaatagta ccctaatgaa gtttttctgt gcatgtgatt aatttcatgt aaaccgatga
    48121 caccaactag cgttccttca taccaaatgc cacactgaaa tccattacca tcagcaaatt
    48181 gcaaaagtcc tcttttaata aatgcacgcg tatctgatgg ttgctcagtt gcatctaccc
    48241 aaggtaacca ttccctaagt gaatttcttg aacgattgac taaattgaaa agcgcttctg
    48301 tgtcatgagc ttctaaaatt tttaatgtta tttgttcatt cactttcatt ccaaacatac
    48361 catcacatcc tcattcattt ttcatataat tctgtaaatt atcattataa taacatatta
    48421 atgttacgca ttgtatctta aatttgttgt ccttc
//
LOCUS       DALAGD010000003        42807 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.003, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000003 DALAGD010000000
VERSION     DALAGD010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 42807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42807
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.003"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(443..541)
                     /locus_tag="O2701_000118"
     CDS             complement(443..541)
                     /locus_tag="O2701_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292036.1"
                     /translation="MNWIIELDLWLWCVGAINLINAVNDYANEILL"
     gene            complement(746..1342)
                     /gene="recR"
                     /locus_tag="O2701_000119"
     CDS             complement(746..1342)
                     /gene="recR"
                     /locus_tag="O2701_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCZ4292037.1"
                     /translation="MHYPEPISKLIDSFMKLPGIGPKTAQRLAFHTLDMKEDDVVQFA
                     KALVDVKRELTYCSVCGHITENDPCYICEDKQRDRSVICVVEDDKDVIAMEKMREYKG
                     LYHVLHGSISPMDGIGPEDINIPSLIERLKNDEVSELILAMNPNLEGESTAMYISRLV
                     KPIGIKVTRLAQGLSVGGDLEYADEVTLSKAIAGRTEM"
     gene            complement(1349..1666)
                     /locus_tag="O2701_000120"
     CDS             complement(1349..1666)
                     /locus_tag="O2701_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002479279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCZ4292038.1"
                     /translation="MRGGGNMQQMMKQMQKMQKKMAQEQEKLKEERIVGTAGGGMVAV
                     TVTGHKEVVDVEIKEEAVDPDDIEMLQDLVLAATNEAMNKADELTQERLGKHTQGLNI
                     PGM"
     gene            complement(1756..3453)
                     /gene="dnaX"
                     /locus_tag="O2701_000121"
     CDS             complement(1756..3453)
                     /gene="dnaX"
                     /locus_tag="O2701_000121"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCZ4292039.1"
                     /translation="MNYQALYRMYRPQSFEDVVGQEHVTKTLRNAISKEKQSHAYIFS
                     GPRGTGKTSIAKVFAKAINCLNSTDGEPCNECHICKGITQGTNSDVIEIDAASNNGVD
                     EIRNIRDKVKYAPSESKYKVYIIDEVHMLTTGAFNALLKTLEEPPAHAIFILATTEPH
                     KIPPTIISRAQRFDFKAISLDQIVERLKFVADAQQIECEDEALAFIAKASEGGMRDAL
                     SIMDQAIAFGDGTLTLQDALNVTGSVHDEALDHLFDDIVQGDVQASFKKYHQFITEGK
                     EVNRLINDMIYFVRDTIMNKTSEKDTEYRALMNLELDMLYQMIDLINDTLVSIRFSVN
                     QNVHFEVLLVKLAEQIKGQPQVIANVAEPAQIASSPNTDVLLQRMEQLEQELKTLKAQ
                     GVSVAPVQKSSKKPARGIQKSKNAFSMQQIAKVLDKANKADIKLLKDHWQEVIDHAKN
                     NDKKSLVSLLQNSEPVAASEDHVLVKFEEEIHCEIVNKDDEKRSSIESVVCNIVNKNV
                     KVVGVPSDQWQRVRTEYLQNRKNEGDDMPKQQAQQTDIAQKAKDLFGEETVHVIDEE"
     gene            complement(3522..4046)
                     /locus_tag="O2701_000122"
     CDS             complement(3522..4046)
                     /locus_tag="O2701_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCZ4292040.1"
                     /translation="MQIYLSTLTELDYDKSLNSIEESFDDNPETSWQARAKVKHLRKS
                     PCYNFELEVIAKNENNDVVGHVLLIEVEINSDDKTYYGLAIASLSVHPELRGQKLGRG
                     LVQAVEERAKAQEYSTVVVDHCFDYFEKLGYQNAAEHDIKLESGDAPLLVKYLWDNLT
                     DAPHGIVKFPEHFY"
     gene            complement(4184..4285)
                     /locus_tag="O2701_000123"
     CDS             complement(4184..4285)
                     /locus_tag="O2701_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292041.1"
                     /translation="MKTIQRIIRGTCLWEVAFLYVKFDSSELDVQFE"
     gene            complement(4354..4623)
                     /gene="ffs"
                     /locus_tag="O2701_000124"
     ncRNA           complement(4354..4623)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="O2701_000124"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(4685..5413)
                     /gene="treR"
                     /locus_tag="O2701_000125"
     CDS             complement(4685..5413)
                     /gene="treR"
                     /locus_tag="O2701_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HCZ4292042.1"
                     /translation="MAKQKKFMKIYEALKEDILNGQIQYGEQIPSEHDLVQLYQSSRE
                     TVRKALDLLALDGMIQKIHGKGSLVIYQEVTEFPFSELVSFKEMQEEMGVAYLTEVVV
                     NEVVEAHEVPEVQHALNINSSESLIHIVRTRRLNQHVKIVDEDYFLKSIVSDIGNDVA
                     SDSIYDYLEKVLNLNISYSSKSITFEPFDEQAYQLFGDVSVAYSATVRSIVYLENTMP
                     FQYNISKHLANEFKFNDFSRRRIK"
     gene            complement(5438..7078)
                     /gene="treC"
                     /locus_tag="O2701_000126"
     CDS             complement(5438..7078)
                     /gene="treC"
                     /locus_tag="O2701_000126"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCZ4292043.1"
                     /translation="MSKEIDWRKSVVYQIYPKSFNDTTGNGIGDINGIIEKLDYIKLL
                     GVDYIWLTPVYESPMNDNGYDISNYLEINEDFGTMDDFEKLIKVAHQKDLKVMLDIVI
                     NHTSTEHEWFKEARKSKDNPYRDYYFFRSSEDGPPTNWYSKFGGNAWKYDSETDEYYL
                     HLFDVSQADLNWDNPEVRQSLYRIVNHWIDFGVDGFRFDVINLISKGEFKDSDKIGKE
                     FYTDGPRVHEFLHELNRQTFGNTDMMTIGEMSSTTIENCIKYTQPERQELNSVFNFHH
                     LKVDYVDGEKWTNAKLDFHKLKEILMQWQRGIYDGGGWNAIFWCNHDQPRVVSRFGDD
                     TSEEMRIQSAKMLAIALHMLQGTPYIYQGEEIGMTDPHFTSIAQYRDVESINAYHQLL
                     SEGHAEADVLAILGQKSRDNSRTPMQWSDDVNAGFTAGKPWIDISENYHQVNVRQALQ
                     NKESIFYTYQKLIQLRHTHDIITYGDIVPRFMDHDHLFVYERHYKNQQWLVIANFSAS
                     AVDLPEGLAREGCVVIQTGTVENNTISGFGAIVIETNA"
     gene            complement(7142..8569)
                     /gene="treP"
                     /locus_tag="O2701_000127"
     CDS             complement(7142..8569)
                     /gene="treP"
                     /locus_tag="O2701_000127"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HCZ4292044.1"
                     /translation="MAVKREDVKAIVTAIGGKENLEAATHCVTRLRLVLKDESKVDKD
                     ALSNNALVKGQFKADHQYQIVIGPGTVDEVYKQFIDETGAQEASKDEAKQAAAQKGNP
                     VQRLIKLLGDIFIPILPAIVTAGLLMGINNLLTMKGLFGPKALIEMYPQIADISNIIN
                     VIASTAFIFLPALIGWSSMRVFGGSPILGIVLGLILMHPQLVSQYDLAKGNIPTWNLF
                     GLEIKQLNYQGQVLPVLIAAYVLAKIEKGLNKVVHDSIKMLVVGPVALLVTGFLAFII
                     IGPVALLIGTGITSGVTFIFQHAGWLGGAIYGLLYAPLVITGLHHMFLAVDFQLMGSS
                     LGGTYLWPIVAISNICQGSAAFGAWFVYKRRKMVKEEGLALTSCISGMLGVTEPAMFG
                     VNLPLKYPFIAAISTSCVLGAIVGMNNVLGKVGVGGVPAFISIQKEFWPVYLIVTAIA
                     IVVPCILTIVMSHFSKQKAKEIVED"
     gene            complement(9040..9132)
                     /locus_tag="O2701_000128"
     tRNA            complement(9040..9132)
                     /locus_tag="O2701_000128"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9094..9096),aa:Ser,seq:tga)
     gene            complement(9401..10864)
                     /locus_tag="O2701_000129"
     CDS             complement(9401..10864)
                     /locus_tag="O2701_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HCZ4292045.1"
                     /translation="MGEFKGFMKYDKQYLGELSLVDRLKHHKAYQQRFTKEDASIQGA
                     RCMDCGTPFCQTGQQYGRETIGCPIGNYIPEWNDLVYHQDFKTAYERLSETNNFPDFT
                     GRVCPAPCESACVMKINRESIAIKGIERTIIDEAFENGWVAPKVPSRRRDEKVAIVGS
                     GPAGLAAAEELNLLGYQVTIYERARESGGLLMCGIPNMKLDKDVVRRRIKLMEEAGIT
                     FINGVEVGVDIDKATLESEYDAIILCTGAQKGRDLPLEGRMGDGIHFAMDYLTEQTQL
                     LNGEIDDITITAKDKNVIIIGAGDTGADCVATALRENCKSIVQFNKYTKLPEAITFTE
                     NASWPLAMPVFKMDYAHQEYEAKFGKEPRAYGVQTMRYDVDDKGHIRGLYTQILEQGE
                     NGMVMKEGPERFWPADLVLLSIGFEGTEPTVPNAFNIKTDRNRIVADDTNYQTNNEKV
                     FAAGDARRGQSLVVWAIKEGRGVAKAVDQYLASKVCV"
     gene            complement(10882..15381)
                     /gene="gltB"
                     /locus_tag="O2701_000130"
     CDS             complement(10882..15381)
                     /gene="gltB"
                     /locus_tag="O2701_000130"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCZ4292046.1"
                     /translation="MHNEKLIKGLYDYREEHDACGIGFYANMDNKRSHDIIDKSLEML
                     RRLDHRGGVGADGITGDGAGIMTEIPFAFFKQHVTDFDIPGEGEYAVGLFFSKERILG
                     SEHEVVFKKYFEGEGLSILGYRNVPVNKDAIAKHVADTMPVIQQVFIDIRDIEDVEKR
                     LFLARKQLEFYSTQCDLELYFTSLSRKTIVYKGWLRSDQIKKLYTDLSDDLYQSKLGL
                     VHSRFSTNTFPSWKRAHPNRMLMHNGEINTIKGNVNWMRARQHKLIETLFGEDQHKVF
                     QIVDEDGSDSAIVDNALEFLSLAMEPEKAAMLLIPEPWLYNEANDANVRAFYEFYSYL
                     MEPWDGPTMISFCNGDKLGALTDRNGLRPGRYTITKDNFIVFSSEVGVVDVPESNVAF
                     KGQLNPGKLLLVDFKQNKVIENNDLKGAIAGELPYKAWIDNHKVDFDFENIQYQDSQW
                     KDETLFKLQRQFAYTKEEIHKYIQELVEGKKDPIGAMGYDAPIAVLNERPESLFNYFK
                     QLFAQVTNPPIDAYREKIVTSELSYLGGEGNLLAPDETVLDRIQLKRPVLNESHLAAI
                     DQEHFKLTYLSTVYEGDLEDALEALGREAVNAVKQGAQILVLDDSGLVDSNGFAMPML
                     LAISHVHQLLIKADLRMSTSLVAKSGETREVHHVACLLAYGANAIVPYLAQRTVEQLT
                     LTEGLQGTVVDNVKTYTDVLSEGVIKVMAKMGISTVQSYQGAQIFEAIGLSHDVIDRY
                     FTGTQSKLSGISIDQIDAENKARQQSDDNYLASGSTFQWRQQGQHHAFNPESIFLLQH
                     ACKENDYAQFKAYSEAVNKNRTDHIRHLLEFKACTPIDIDQVEPVSDIVKRFNTGAMS
                     YGSISAEAHETLAQAMNQLGGKSNSGEGGEDAKRYEVQVDGSNKVSAIKQVASGRFGV
                     TSDYLQHAKEIQIKVAQGAKPGEGGQLPGTKVYPWIAKTRGSTPGIGLISPPPHHDIY
                     SIEDLAQLIHDLKNANKDADIAVKLVSKTGVGTIASGVAKAFADKIVISGYDGGTGAS
                     PKTSIQHAGVPWEIGLAETHQTLKLNDLRSRVKLETDGKLLTGKDVAYACALGAEEFG
                     FATAPLVVLGCIMMRVCHKDTCPVGVATQNKDLRALYRGKAHHVVNFMHFIAQELREI
                     LASLGLKRVEDLVGRTDLLQRSSTLKANSKAASIDVEKLLCPFDGPNTKEIQQNHNLE
                     HGFDLTNLYEVTKPYIAEGRRYTGSFTVNNEQRDVGVITGSEISKQYGEAGLPENTIN
                     VYTNGHAGQSLAAYAPKGLMIHHTGDANDYVGKGLSGGTVIVKAPFEERQNEIIAGNV
                     SFYGATSGKAFINGSAGERFCIRNSGVDVVVEGIGDHGLEYMTGGHVINLGDVGKNFG
                     QGMSGGIAYVIPSDVEAFVENNQLDTLSFTKIKHQEEKAFIKQMLEEHVSHTNSTRAI
                     HVLKHFDRIEDVVVKVIPKDYQLMMQKIHLHKSLHDNEDEAMLAAFYDDSKTIDAKHK
                     PAVVY"
     gene            15562..16446
                     /locus_tag="O2701_000131"
     CDS             15562..16446
                     /locus_tag="O2701_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCZ4292047.1"
                     /translation="MEIKQLRYFIEVAKREHISETALELNIAQSAISRQITLLEQELN
                     VSLFKKQGRNITLTSEGKLLFNEALRIIEHLDSTIEQFQSHGLTKNKSIYIGYDESDV
                     SHMLLPLIQTFHLQNDTHVIPSLLEHDTIINSVLNGNIDIGFTELTPEIRKHKQLHML
                     PLFEEHYHLYAPSDDPITMATHPPLIQFEHSHIYCLAPFAETVKKQLRKITKSDVYTI
                     SSQPLAQYLLRQKEGYIISSQNIHLPESKSWIDIKLDHTELKRTICAITKEPYTKSDI
                     GILLTLIQQLMTKTSTFH"
     gene            16561..17679
                     /locus_tag="O2701_000132"
     CDS             16561..17679
                     /locus_tag="O2701_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCZ4292048.1"
                     /translation="MFLKYRNLFTKPFNIILGLFCIAFIGAILFTFNNENFYNKPIGQ
                     IIDVKHVSSTPTKDAQNNRDIKYKNQLKVKILNGQFAGETTTINHQYVKSQADSEAFR
                     THEKVLLHISNKPSDAYIIEKKRDTLTVIITGLFLLTVLLVGRKVGLQSILSLILNSI
                     AILIAIYIHIQHSNINLFLLMTIAMICSTILTLLLVTGWHMRTLITIASTIIGTFLSI
                     GLTELIIYMADGKGIKYETMNFLSLPPKDIFLASVLIGSLGAIMDVAITIASGMHEIL
                     QRTPHISMRRWALAGRNIGQDIMGTMTNILLFSYLSGALPMFLIFLKNANTVTYTISM
                     NWSLEIARALTGGIGIVLTIPITILFMEIFETLRRAKQ"
     gene            17676..18458
                     /locus_tag="O2701_000133"
     CDS             17676..18458
                     /locus_tag="O2701_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCZ4292049.1"
                     /translation="MNAVFILALLLLFLMIIFGGKKGFISYLTLFLNFAILIISIVLI
                     IFGVPIYVVTFFFCIIIGACNLFVLNSYNVKTQAAFISTIVTTILLITLIYLSVYFGH
                     LQGFSTEQQDETYVYSMNIGIDMVQFMVFTIILAVIAAVIDLTITISSPIYELHQVNP
                     TLTQYELFQSGMRVGREILATSANTIYLAFFGGQLALFIWFFKLKYSFGHIINSKIFA
                     QEFISIILGGIAVAISIPITAWITAFLIKRPSRQRKNDESIN"
     gene            complement(18627..19112)
                     /locus_tag="O2701_000134"
     CDS             complement(18627..19112)
                     /locus_tag="O2701_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4292050.1"
                     /translation="MTNNDTIMLRHYVPQDYSMLEAFQLSESDLKFVKTPEENITAAM
                     SDNERYPIVVMDGRQCVAFFTLHRGKGVAPFSDNQDAVFFRSFSVDQRYRNRGIGKVV
                     MEKLASFITSTFQDINEIVLTVNTDNPHAMALYRQQGYQYMGDSMFVGRPVHIMALTI
                     K"
     gene            complement(19102..19497)
                     /locus_tag="O2701_000135"
     CDS             complement(19102..19497)
                     /locus_tag="O2701_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCZ4292051.1"
                     /translation="MIKCVCLVEETADKILLVQVRNREKYYFPGGKIEEGESQVHALL
                     REVKEELNLTLTMDEIEYVGTIVGPAYPQQDMLTELNGFRALTKIDWENVTINNEITD
                     IRWIDKDNDALIAPAVKVWIETYGGKHDK"
     gene            19646..19915
                     /locus_tag="O2701_000136"
     CDS             19646..19915
                     /locus_tag="O2701_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292052.1"
                     /translation="MAKRSKSQRLSSLLNVAGFIVDGYNGYKYHAKNKKLVYLSLGLS
                     TVGTVLDFYISIKSPRKFKKAVAVVTLITNGARLFTSIRKVKHEY"
     gene            complement(20100..21104)
                     /gene="aaa"
                     /locus_tag="O2701_000137"
     CDS             complement(20100..21104)
                     /gene="aaa"
                     /locus_tag="O2701_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin/adhesin Aaa"
                     /protein_id="HCZ4292053.1"
                     /translation="MQKKVIAAIIGTSAISAVAATQANAATTHTVKPGESVWAISNKY
                     GISIAKLKSLNNLTSNLIFPNQVLKVSGSSNSTSNSSRPSTNSGGGSYYTVQAGDSLS
                     LIASKYGTTYQNIMRLNGLNNFFIYPGQKLKVSGTASSSNAASNSSRPSTNSGGGSYY
                     TVQAGDSLSLIASKYGTTYQKIMSLNGLNNFFIYPGQKLKVTGNASTNSGSATTTNRG
                     YNTPVFSHQNLYTWGQCTYHVFNRRAEIGKGISTYWWNANNWDNAAAADGYTIDNRPT
                     VGSIAQTDVGYYGHVMFVERVNNDGSILVSEMNYSAAPGILTYRTVPAYQVNNYRYIH
                     "
     gene            complement(21436..22278)
                     /gene="gmpC"
                     /locus_tag="O2701_000138"
     CDS             complement(21436..22278)
                     /gene="gmpC"
                     /locus_tag="O2701_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter
                     glycylmethionine-binding lipoprotein"
                     /protein_id="HCZ4292054.1"
                     /translation="MKRLIGLVIVALVLLAACGGNNDKKVTIGVASNDTKAWEKVKEL
                     AKKDDIDVEIKHFSDYNLPNKALNDGDIDMNAFQHFAFLDQYKKAHKGTKISALSTTV
                     LAPLGIYSDKIKDVKKVKDGAKVVIPNDVSNQARALKLLEAAGLIKLKKDFGLAGTVK
                     DITSNPKHLKITAVDAQQTARALSDVDIAVINNGVATKAGKDPKNDPIFLEKSNSDAV
                     KPYINIVAVNDKDLDNKTYAKIVELYHSKEAQKALQEDVKDGEKPVNLSKDEIKAIET
                     SLAK"
     gene            complement(22315..22974)
                     /locus_tag="O2701_000139"
     CDS             complement(22315..22974)
                     /locus_tag="O2701_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4292055.1"
                     /translation="MFGSDLDSAQLLQALYETLYMVSIALFLGAVIGIPLGVLLVITR
                     KQGIWPNIVIHQVLNPLINILRSLPFIILLIAIVPFTKLVVGTSIGTTAAIVPLTVYV
                     APYIARLVENSLLEVDEGIIEAAKAMGASPLQIIRYFLIPEALGSLVLAITTAIIGLI
                     GSTAMAGAVGGGGIGDLALVYGYQRFDTTVIIITVIVLVIIVQVIQTLGNVLARFIRR
                     H"
     gene            complement(22978..24003)
                     /locus_tag="O2701_000140"
     CDS             complement(22978..24003)
                     /locus_tag="O2701_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HCZ4292056.1"
                     /translation="MIEFRQVSKTFNKKKQKIDALKDVSFTVNRNDIFGVIGYSGAGK
                     STLVRLVNHLEAASNGQVIVDGHDITNYSDKMMRDIKKDIGMIFQHFNLLNSATVFKN
                     VAMPLILSKKSKTEIKQRVTEMLEFVGLSDKKDQFPDELSGGQKQRVAIARALVTNPK
                     ILLCDEATSALDPATTASILTLLKNVNQTFGITIMMITHEMRVIKDICNRVAVMEKGK
                     VVETGTVKEVFSHPKTTIAQNFVSTVIQTEPSTSLIRRLNDEQVGDFKDYKIFVEETQ
                     VTQPIINDLIQICGREVKILFSSMSEIQGNTVCYMWLRFNMDQQFEDTAINQYFKEKN
                     IQFEEVH"
     gene            complement(24297..25439)
                     /locus_tag="O2701_000141"
     CDS             complement(24297..25439)
                     /locus_tag="O2701_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional cystathionine
                     gamma-lyase/homocysteine desulfhydrase"
                     /protein_id="HCZ4292057.1"
                     /translation="MNKKTKLIHGGHTTDDYTGAVTTPIYQTSTYLQDDIGDLRQGYE
                     YSRTANPTRSSVESVIATLENGKHGFAFSSGVAAISAVVMLLDKGDHIILNSDVYGGT
                     YRALTKVFTRFGIEVDFVDTTHTDSIVQAIRPTTKMLFIETPSNPLLRVTDIKKSAEI
                     AKEHGLISVVDNTFMTPYYQNPLDLGIDIVLHSATKYLGGHSDVVAGLVATSDDKLAE
                     RLAFISNSTGGILGPQDSYLLVRGIKTLGLRMEQINRSVIEIIKMLQAHPAVQQVFHP
                     SIESHLNHDVHMAQADGHTGVIAFEVKNTESAKQLIKATSYYTLAESLGAVESLISVP
                     ALMTHASIPADIRAKEGITDGLVRISVGIEDTEDLVDDLKQALDTL"
     gene            complement(25423..26337)
                     /locus_tag="O2701_000142"
     CDS             complement(25423..26337)
                     /locus_tag="O2701_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HCZ4292058.1"
                     /translation="MITYDLIGNTPLVLLEHYSDDKVKIYAKLEQWNPGGSVKDRLGK
                     YLVEKAIQEGRVRAGQTIVEATAGNTGIGLAIAANRHHLKCKIFAPYGFSEEKINIMI
                     ALGAEVSRTSQSEGMHGAQLAARSYAEKYGAVYMNQFESEHNPDTYFHTLGPELTSAL
                     QQIDYFVAGIGSGGTFTGTARYLKQHHVQCYAVEPEGSVLNGGPAHAHDTEGIGSEKW
                     PIFLERRLVDGIFTIKDQDAFRNVKSLAINEGLLVGSSSGAALQGALNLKAQLSEGTI
                     VVVFPDGSDRYMSKQIFNYEENNNEQEN"
     gene            complement(26554..27891)
                     /locus_tag="O2701_000143"
     CDS             complement(26554..27891)
                     /locus_tag="O2701_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HCZ4292059.1"
                     /translation="MKRQQSQWKSSTGFILASAGSAIGLGAMWKFPYMAGIYGGGAFL
                     AMFLIFTIFVGLPLLIMEFTVGKMGRTYTTQIYSKLTGKKWLNIIGWNGNLAVFILFG
                     FYSVIGGWIVIYIGQVLWQLVIFQRINHLQEMNFEAVISNPWLTVLGQGIFIFATMII
                     VMLGVEKGLEKASKVMMPLLFVFLIVIVIKSLTLDGALEGVKFILQPRVSEITADGIL
                     FALGQSFFTLSLGTTGMITYASYASKDMTIKSSAISIVVMNIFVSVLAGLAIFPALHS
                     FGYEPQEGPGLLFKVLPMVFSQMHLGTLFYLGFLVLFLFAALTSSISLLELNVSNFTK
                     NDNTKRKKVAVIGSILVFIISIPATLSFGILKDVRFGAGTIFDNMDFIVSNVLMPLGA
                     LGTTLVVGQLLDKKLLQQYFGKDRFRLFSGWYYLIKYAMPVVIILVFIVQLFS"
     gene            complement(28056..28169)
                     /locus_tag="O2701_000144"
     CDS             complement(28056..28169)
                     /locus_tag="O2701_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292060.1"
                     /translation="MGIAPEVVMWRDVGDIDGVVCGEILAHVYSVEPENLM"
     gene            complement(28347..29081)
                     /locus_tag="O2701_000145"
     CDS             complement(28347..29081)
                     /locus_tag="O2701_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HCZ4292061.1"
                     /translation="MRIKTPSPSYLKGTNGHAILLLHSFTGTNRDVKHLAAELNDQGF
                     SCYAPNYPGHGLLLKDFMTYNVDDWWEEVEKAYQFLVNEGYESISATGVSLGGLMTLK
                     LAQHYPLKRIAVMSAPKEKSDDGLIEHLVYYSQRMSNILNLDQQASSAQLAAIDDYEG
                     EITKFQHFIDDIMTNLNVIKMPANILFGGKDAPSYETSAHFIYEHLGSVDKELNGLKD
                     SHHLMTHGEGRDILEENVIRFFNALT"
     gene            complement(29118..29792)
                     /locus_tag="O2701_000146"
     CDS             complement(29118..29792)
                     /locus_tag="O2701_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCZ4292062.1"
                     /translation="MIDKKLTSPKMTVPLFLIALIVFIGMFYSVVTNQEWLKNIDMGS
                     LTWFTDYFGEPQRQYVNNLFNYYMTFSAEIGDVKGVVLISIIVTIILFIKQRHLAVWF
                     VTYLVSGVIMNKLIKDTVLRPRPYNHLAVDTGFSFPSGHSNASTLLYFALMIIIISLA
                     AKTITKVLSALVMGILWLSILFCRLYFHAHYFSDVIGGTSLAIIWVALFLMVYPYFIN
                     HRRQRV"
     gene            29830..30246
                     /locus_tag="O2701_000147"
     CDS             29830..30246
                     /locus_tag="O2701_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292063.1"
                     /translation="MLSIISMCHKFHFTDNIKIIKFILLCILQIKHCIIEITIFCYFQ
                     FSTIYIYYTEGVRRINRVIFVYIALIITLVRFFTPIHPSFSNLIYWIFVLYFIPIILC
                     VIGFKAEKLIATMVIIPNFLGILYRLYAYVTHILFM"
     gene            complement(30475..30837)
                     /locus_tag="O2701_000148"
     CDS             complement(30475..30837)
                     /locus_tag="O2701_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HCZ4292064.1"
                     /translation="MKRTKGEIEAEISKAITQWEKDFLGRGSLSVKSDILRDMVIISL
                     QGILTPAEYRVCSTNEGLLNIKRTRSELVESGEQDLNDIIFKITGIKVMSFHSDLSTV
                     TGERIIVFKLEDNLEKHI"
     gene            complement(30999..33704)
                     /locus_tag="O2701_000149"
     CDS             complement(30999..33704)
                     /locus_tag="O2701_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcC family protein"
                     /protein_id="HCZ4292065.1"
                     /translation="MTTQLNINSVIENAKRVITPLSPISIFAARNPWEGLEADTFEDV
                     AKWLRDVRDVDIFPNKALIESAVARGELDESVFNQLVTDMLLEHHYNIPQHYINLYID
                     NIKTLKDVPASYMNHSNVDVVADLLLEKSKRDMAESYHHYDVRPMSDAIIDEQGEPLS
                     EQVNRQMIKWTKLYIDQFLSSWTMPKREQSFYHAWLHLAQHDHSFTKAQRQVIKGLPN
                     DPEMTIESVLTHFSIDQEDYQAYVEGHLLALPGWAGMLYYRSQQHYFEQHLLTDYLAI
                     RLVVEQLLVGDEFKSVAKDCESRSENWFKQTVASWCYYSDMPSDVLLQHDVNEIQTFI
                     HFAATMNKNVFKNLWLIAWEMTYESQLKQKIKAGHESVAGALDVNQVNVSENDNANQP
                     HSVLLNDTQAVDENNSELNQMGTSTKAQIAFCIDVRSEPFRRHIEAAGPFETIGIAGF
                     FGLPIQKDAVDEQFKHDSLPVMVPPAYRIKEFADRYDMNVYRQQQQTMSSMFYTFKLM
                     KNNVMPSLLLPELSGPFLSLSTIVNSIMPRKSRASLQKIKQKWLKKPETKLTIDREFD
                     RTSDLPVGFTEQEQIDFALQALKLMDLTEAFAPFVVLAGHASHSHNNPHHASLECGAC
                     GGASSGFNAKLLAMICNRPNVRQGLKQSGVYIPETTVFAVAEHHTSTDTLAWVYVPDT
                     LSSIALDAYESLNDAMPMISEHANRERLDKLPTIGRVNHPVEEAQRFASDWSEVRPEW
                     GLAKNASFIIGRRQLTKGIDLEGRTFLHNYDWRKDKDGTLLNTIISGPALVAQWINLQ
                     YYASTVAPHFYGSGNKATQTVTSGVGVMQGNASDLMYGLSWQSVMAADRTMYHSPIRL
                     LVVIQAPDYVVARLLANNEHFARKVSNHWLRLMSVNEEGRFKSWI"
     gene            complement(33717..35201)
                     /locus_tag="O2701_000150"
     CDS             complement(33717..35201)
                     /locus_tag="O2701_000150"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase subunit 5"
                     /protein_id="HCZ4292066.1"
                     /translation="MLSFQLLFSLFVIALIIALISGLLFLAPVMPMRYIKLHLYILVM
                     PVLFAVIGFFGIHGQHVLGPFKIDRLSWLLAGFVMALGFIIQKFSMRYLLGDHHYRHY
                     FPLFTAITSFASLAWMSEDLRLMALCWGITLLCLTLLMNVNRFWKVPRESAKLSSMTF
                     LCGWLAFVGAIVTIYIATGEWRVPQHIVHPTWSLLTNVLLVLAVMIPAAQFPFHRWLI
                     ESVTAPTPVSAIMHAGIVNAGGVILTRFAPIFDNGFALSLLLILSSISVLLGSGISLV
                     QVDYKRQLVGSTMSQMGFMLVQCALGVYSAAIIHLILHGIFKATLFLQSGSIVKRFNI
                     PKQASAKDAYGWIVMGRVLAIIVAFLFWMSSDRSAYEVLSALILGWSLLVSWNQMVAF
                     SKGRMARLVGMILIAIVTFIYVITHNYFYAVLQNITTHATTPPTVSVIISVVILIFGS
                     LLSIWVARHRYSKAFAVLYVWLVNLGEARSKAIESHPNYLKKYL"
     gene            35722..35787
                     /locus_tag="O2701_000151"
     CDS             35722..35787
                     /locus_tag="O2701_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-1"
                     /protein_id="HCZ4292067.1"
                     /translation="MGIIAGIIKVIKSLIEQFTGK"
     gene            35819..35884
                     /locus_tag="O2701_000152"
     CDS             35819..35884
                     /locus_tag="O2701_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-2"
                     /protein_id="HCZ4292068.1"
                     /translation="MGIIAGIIKFIKGLIEKFTGK"
     gene            35936..36004
                     /locus_tag="O2701_000153"
     CDS             35936..36004
                     /locus_tag="O2701_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-3"
                     /protein_id="HCZ4292069.1"
                     /translation="MEFVAKLFKFFKDLLGKFLGNN"
     gene            36068..36130
                     /locus_tag="O2701_000154"
     CDS             36068..36130
                     /locus_tag="O2701_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-4"
                     /protein_id="HCZ4292070.1"
                     /translation="MAIVGTIIKIIKAIIDIFAK"
     gene            complement(36789..37991)
                     /locus_tag="O2701_000155"
     CDS             complement(36789..37991)
                     /locus_tag="O2701_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCZ4292071.1"
                     /translation="MAKIPVTVLSGYLGSGKTTLLNHILQNREGRRIAVIVNDMSEVN
                     IDKDLVADGGGLSRTDEKLVELSNGCICCTLRDDLLKEVERLVKKGGIDQIVIESTGI
                     SEPVPVAQTFSYIDDELGIDLTAICRLDTMVTVVDANRFVHDINSEDLLMDRDQSVDE
                     TDERSIADLLIDQVEFCDVLIINKIDLISEEELAKLEKMLSALQPTAKIIKTTNSEVD
                     LKEVLNTQRFDFEKASESAGWIKELESGGHASHTPETEEYGISSFVYKRRLPFHAKRF
                     NDWLESMPNNVVRSKGIVWLAQYNHVACLLSQAGSSCNIHPVTYWVASMSEAQQTQIL
                     AERQDVAAEWDPEYGDRHTQFVIIGTELDEEKLTKELDACLVNAQEIDADWQQFEDPY
                     QWQIRPAR"
     gene            38110..38370
                     /locus_tag="O2701_000156"
     CDS             38110..38370
                     /locus_tag="O2701_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292072.1"
                     /translation="MSALLYNGDKDISHERESRCINMLFVILVLYVTGIAFILLSVFG
                     SKTEGLSTKHTLYTIGSAIITIAIFISIGYAIQYLTAALYGL"
     gene            complement(38660..38974)
                     /locus_tag="O2701_000157"
     CDS             complement(38660..38974)
                     /locus_tag="O2701_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292073.1"
                     /translation="MTLIEPDMTLRMPDISTTVETLNLISKMEAQKENIRTVIAPEHK
                     HKYKDIENGLKGEEKVLIEQMAQHCEAFKANFKGAAQGDWVKSAMSEIDSIKDDLKKI
                     NS"
     gene            complement(38993..39316)
                     /locus_tag="O2701_000158"
     CDS             complement(38993..39316)
                     /locus_tag="O2701_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292074.1"
                     /translation="MMFNQINNKNELEESYESEKKRIENELQNLNELRHRTRKENERS
                     YDVFQYLKHEMNYSEDAQRKMTRNIEAYEQEINEIIRKQEWKLEEYKEDLKKSYEKQL
                     DKLSD"
     gene            complement(39313..40641)
                     /locus_tag="O2701_000159"
     CDS             complement(39313..40641)
                     /locus_tag="O2701_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292075.1"
                     /translation="MLSRKYKIDLKAINQNTESTSAISKASYEVENANNNGLSKRDVI
                     NQFNDLKKMKKFPSNLEYVDSYTDSLTGVTTSAFLNKDTGKVTLGMTGTNLQDEAFKK
                     LKEGEFSRQNVTNALETVKDGYADLKILYSPASDQNYRYANTQEFINKIKSKYDIDFI
                     TGHSLGGRDAVVLGMSNGIPNIVVYNPAPISITSLNPNSPDGKRLLELYKNYKGNITR
                     FVAENDALTENLKKYKHYVFFGNDKVFKNGKGHEMEGFLTEEEQKAIKKELKKLQGYA
                     EENNKSFVKNSNNAISKLASIELLRANMMTTNGGGLSSSQQKVLESLTALTIAQSFSQ
                     LIDDEINQIKKMYNEKKKKFGKNWEDAQKAGKAVGEDLSVNGVLNALDEGQVNESSMV
                     REPEQMISAKERQLSTIGSSVSNYIMRVRLSINEIVDKDQVLASQIGGLL"
     gene            complement(40631..41443)
                     /gene="lpl10"
                     /locus_tag="O2701_000160"
     CDS             complement(40631..41443)
                     /gene="lpl10"
                     /locus_tag="O2701_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl10"
                     /protein_id="HCZ4292076.1"
                     /translation="MEYIKKIALYMSVLLLIIFIGGCGNMKDEQKKEEQTNKTDSKEE
                     QIKKSFAKTLDMYPIKNLEDLYDKEGYRDGEFKKGDKGTWTILTGFSKSNKPGVLDDE
                     GMVLYLNRNAKKATGYYFVNKVYDDISKNHNEKKYRVELKNNKIVLLDNVEDKKLKQK
                     IENFKFFSQYADFKDLKNYQDGNITTNENVPSYEAQYKMNNSDKNVKKLREIYPITTN
                     NSPNLKLYIDGDIKGSSVGYKKIEYKFSKDKGQETTLRDYLNFGPSEGENVE"
     gene            complement(41626..42411)
                     /gene="lpl9"
                     /locus_tag="O2701_000161"
     CDS             complement(41626..42411)
                     /gene="lpl9"
                     /locus_tag="O2701_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl9"
                     /protein_id="HCZ4292077.1"
                     /translation="MKSIKRIGLCISLLILIIFVTSCDGDNKIIGDSKEEQIKKSFAK
                     TLDIYPIKNLEDLYDKEGYRDGEFKKDDKGTWLIRSEMKIQLKGENLESRGAVLEINR
                     NTRTAKGHYIVREVVEDSDGMTHNHTKRYPVKMENNKMIPLKPIDDEKVKKEIEEFNF
                     FVQYGNFKELENYKEDEVSYNPEVPIYSAKYQLKNSDYNVEQLRKRYNIPTQKAPKLL
                     LKGSGNLKGSSVGYKNIEFTFIENKEENIYFTDSIYFNPSEDK"
     gene            complement(42430..>42807)
                     /gene="lpl8"
                     /locus_tag="O2701_000162"
     CDS             complement(42430..>42807)
                     /gene="lpl8"
                     /locus_tag="O2701_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl8"
                     /protein_id="HCZ4292078.1"
                     /translation="ILLDKVEDTNLKKRIENFKFFGQYANLKELKNYNNGDVSINENV
                     PSYDAKFKMSNKDENVKQLRSRYNIPTDKAPVLKMHIDGNLKGSSVGYKKLEIDFSKG
                     GKSDLSVIDSLNFQPAKVDEDDE"
BASE COUNT        13356 a         8748 c         6109 g        14594 t
ORIGIN      
        1 tttcttaccg acaagaatta attatacatt ttattaacat ttaagtcaat aactttttaa
       61 aattaatttt cgttagtaat tcaagttatt tcttaagttg agttgttttg tttgcctcaa
      121 caaataaata ctatacaggc tttcaaactg cttaacaaca gtaaaaatta cacctttgtc
      181 atctgtactg aacgccctta tcctaaaaat cgaacttaaa aaatccccac ttataaatag
      241 tacgtttttt agaacaagaa acagattttt ctctaaccat atcaatcaaa aaaaacgcaa
      301 aacagaacgt tattttttta ccagaaatta aaaataaatt tacaagtttt aaataatctc
      361 tcaaaacacc gctatacagg ctgatttaat aagctttaga ccaaagaagt acaacttact
      421 aagccagcat ctatcatttc aattacaaaa gaatttcatt tgcatagtca ttaaccgcat
      481 ttatcaaatt aatagcacca acacaccata accatagatc taattcgata atccaattca
      541 catcattact attagaagta acaaatcaca accaacggct tgcttcctaa agattaccca
      601 tccctcagta aaactaggta aaccatacca ccgccacacc gcaaataaag tagtacatca
      661 caccacacca taaaaaaagc cacaatgatt acactgtgac ttttcttact atagtattaa
      721 aatcaaaaat gtttaataga agacattaca tttctgttct acctgcgatt gctttagata
      781 atgttacttc gtcagcatac tctaaatcgc cacctaccga taacccttgt gctaatctcg
      841 tcactttgat acctataggc ttaactaatc tagaaatata catggctgta gattccccct
      901 ctaagttcgg gttcatagct aagattaatt cgctaacttc atcgtttttc aagcgttcaa
      961 tcaatgaagg aatattaata tcttctggtc caatgccatc cataggcgaa atagacccat
     1021 gtaaaacgtg atataaacct ttgtattctc tcattttttc catagctatg acatctttgt
     1081 catcttccac aacacaaata actgaacgat ctctttgctt atcttcacaa atataacatg
     1141 gatcattttc agtaatgtga ccacatacgc tacaatatgt taattctctc ttaacatcta
     1201 ctaatgcttt ggcaaactga acaacatcgt cttctttcat atctaaggta tgaaaagcca
     1261 gacgttgggc tgtctttgga ccaatgcctg gcaatttcat aaagctatca ataagttttg
     1321 atataggttc tggataatgc atctatgatc acattccagg gatgtttaag ccttgagtat
     1381 gtttacctaa acgttcttga gtaagctcat cagctttatt catcgcttca ttagtagctg
     1441 ctaacactaa gtcttgtagc atttcaatat cgtctgggtc tacagcttct tctttgattt
     1501 caacgtcgac aacttcttta tgaccagtta cagtaactgc aaccatgcca ccgccagctg
     1561 ttcctacaat acgctcttct ttaagttttt cttgttcttg agccattttc ttttgcattt
     1621 tttgcatttg tttcatcatt tgttgcatgt ttccgccacc gcgcatattc catttcctcc
     1681 ttgaaaatca ataaatattt atcaataaaa tgatgtttct ttcattatac atacgattat
     1741 atcgcttgtc atgtatcact cttcatctat cacatgtaca gtttcttcac cgaaaagatc
     1801 ttttgctttt tgagcaatat ctgtttgttg tgcttgttgc tttggcatat catcgccttc
     1861 gtttttacga ttttgtaaat attccgttcg aactctttgc cattgatctg atggtacacc
     1921 aacaacttta acgtttttat taacgatatt acatacaaca ctttctatac tactacgttt
     1981 ctcgtcgtct ttattgacga tttcacaatg gatctcttcc tcaaatttca caagtacgtg
     2041 atcttcactt gccgccacag gttccgaatt ttgcaataaa ctaacgagtg attttttatc
     2101 attatttttg gcatgatcaa tcacttcttg ccaatgatct ttcaacaatt tgatatctgc
     2161 cttattcgct ttatctagca cttttgcaat ttgttgcatt gaaaatgcat ttttagattt
     2221 ttgtatgcct ctcgcaggct ttttcgaaga tttttgaaca ggagcgacac tcactccttg
     2281 tgcttttagt gtttttagtt cttgctctaa ctgttccata cgttgcaaca atacatctgt
     2341 gtttggcgat gaagcaattt gtgctggttc agctacattc gcaatcactt gtggttgacc
     2401 cttaatctgc tcagctaatt ttactaacaa cacttcaaaa tgaacgtttt gattcacact
     2461 aaaacgaatc gacactaatg tatcattaat aagatcaatc atttgatata acatatctaa
     2521 ttctaagttc atcagtgctc gatactcagt atctttctca gatgttttat tcataatcgt
     2581 atctctgaca aaataaatca tatcatttat taggcgattc acttctttac cttctgttat
     2641 aaactgatgg tattttttaa aagatgcttg tacgtcacct tgtacaatat catcaaacaa
     2701 gtgatccaac gcttcatcat gtacgctacc tgtgacattc aacgcatctt gcaatgttaa
     2761 cgtaccatca ccaaatgcaa tagcctgatc cataatactt aatgcatcac gcataccccc
     2821 ttcagacgct ttagcgataa atgccaaggc ttcatcttca cattcaattt gttgtgcatc
     2881 tgctacaaat tttaaacgtt caacaatttg atctaggcta attgctttaa aatcaaaacg
     2941 ttgtgcccta gaaatgattg ttggagggat tttatgtggt tctgtcgttg ccaatataaa
     3001 aatagcgtgt gctggaggtt cttctaacgt ctttaaaagg gcattaaaag cacctgttgt
     3061 tagcatgtgc acctcatcta taatataaac tttatatttc gattcacttg gtgcatattt
     3121 aactttgtct ctaatatttc ttatttcatc aaccccatta ttactagcag catcaatttc
     3181 tatcacatct gaattagtcc cctgcgtaat gcctttacaa atatgacatt cattacaagg
     3241 ttctccatca gtgctattta gacagttgat tgctttagca aacactttgg caatactcgt
     3301 tttccccgta cctctcggac cactaaaaat ataagcatgc gactgttttt ctttcgaaat
     3361 cgcattgcgc aatgtcttcg tgacatgttc ttgtccgacg acatcctcga aactttgggg
     3421 tctgtacata cgatataagg cttgataatt caagttagca cctccataaa caattacctc
     3481 tcattatagc atgataatac ctttacttct taattgaaca attaataaaa atgttctgga
     3541 aattttacga ttccgtgtgg tgcatccgtc aaattatccc ataaatattt tacaagtaac
     3601 ggtgcatcac cagattctaa tttaatgtca tgctcagcag cattttgata acccaacttt
     3661 tcaaagtagt caaaacaatg gtctacaaca accgtactat actcttgtgc tttggcacgc
     3721 tcttctactg cttgaaccaa gccacgacct aatttttgtc cacgtaattc aggatgaact
     3781 gataaagagg caatcgccaa accataatac gtcttatcat cactattaat ttctacttca
     3841 attaataaaa cgtgtccaac gacatcgtta ttttcatttt tcgctattac ttctaattca
     3901 aaattatagc aaggagattt tcttaaatgt tttactttcg cacgtgcttg ccaactcgtt
     3961 tcaggattat catcaaaact ttcttcaata ctatttaaag atttatcata atctaactct
     4021 gttaaagtac ttaaatatat ttgcatatgt cctccgtagg catttgattg tcaataatca
     4081 taacgtatct cattaataat tctattgtaa gatactcccc ctcatttcac catcatttct
     4141 ttatcatcaa attataactt cttacttttc attgacacaa aaatcattca aactgcacat
     4201 cgagttcact tgaatcaaac ttcacatata aaaaagctac ttcccacaaa catgttccac
     4261 gtataatacg ctgaattgtc ttcaagaaag tagcttctat aattatatat tttcaactct
     4321 taataatcgg tttaatattt aaaaaataaa aaaccgtgca cctaagcatc gaacgtaatt
     4381 cacgaacgta accaaagtcg ttagctaaat ctcggctacc ctacggcaca tatgatgatc
     4441 cacttaatgc tgcttccgtc aggacctgac atggttcatg ggttcatatt gcataggacc
     4501 gaaatcttca aacactacgt gctttgggca gacttcgcaa aaatacggcc tcaacaaagg
     4561 aattaagcct cgcataaagc ggatttcgag tacagggaac cgctacctcc ccacctagca
     4621 cggcaagata tatattacta tattttaata gttaattgca agtataaatc atttatatca
     4681 ttgtttactt tatacgacgt cttgagaagt cattaaattt aaattcattt gcaagatgtt
     4741 ttgaaatatt atattgaaac ggcattgtat tttctaaata cacaatactt cgaactgttg
     4801 ctgaataagc caccgataca tcaccaaaca attgatatgc ttgttcatca aacggttcaa
     4861 aagtaataga cttacttgaa taactaatat taagatttaa taccttttcc aaataatcat
     4921 aaatagaatc actcgcaaca tcattaccta tatctgaaac aatcgacttt agaaaataat
     4981 cttcatcaac aatcttcaca tgttggttaa gccgacgagt tctaacaata tgaatgagtg
     5041 attcactaga attgatgttt aaagcatgtt gaacttctgg aacttcatgc gcttcaacaa
     5101 cctcattcac aacaacttca gttaaatatg cgacgcccat ttcttcttgc atttctttaa
     5161 aactaacaag ttcagaaaat ggaaactctg taacctcctg ataaatgaca agtgaccctt
     5221 taccatgaat cttttgaatc atgccgtcta atgccaacaa atctaatgcc ttacgcacgg
     5281 tctctcgaga tgactggtac aattgcacca aatcatgttc agacggaatt tgttcaccat
     5341 attgaatctg cccgtttaat atatcttctt tcaacgcctc ataaatcttc ataaactttt
     5401 tttgtttcgc catataaacg catccactca atttatttta cgcgtttgtt tcgattacaa
     5461 ttgcaccaaa cccgcttatc gtattatttt ccactgtgcc tgtttgaatc acaacacaac
     5521 cttctctagc caatccttct ggcaaatcaa cagccgatgc tgagaaattc gcaattacta
     5581 gccattgttg attcttataa tgacgttcat aaacaaataa atgatcatga tccataaaac
     5641 gtggcacaat gtctccatac gtaataatat catgcgtatg tcttaattgt attaattttt
     5701 gatacgtata gaaaatagac tctttattct gaagtgcttg tctaacgttg acctgatgat
     5761 aattttccga aatatcaatc caaggcttac cagctgtaaa tccagcatta acatcatcac
     5821 tccattgcat aggcgttctc gaattgtctc gtgacttctg tcctaaaatc gctaacacat
     5881 ccgcttcagc atgcccttca cttaacaact gatggtaggc attaatcgat tcaacatcac
     5941 gatattgtgc tattgatgta aaatgtgggt ccgtcatacc aatttcttca ccttggtaaa
     6001 tatatggcgt cccttgcaac atatgcagtg cgatagctaa cattttagca ctttgtatcc
     6061 tcatctcttc cgacgtatca tcaccaaatc tagacactac ccgtggctga tcatgattac
     6121 accagaaaat cgcgttccat ccgccaccgt cataaatacc tcgttgccat tgcatcagaa
     6181 tttcctttaa cttatgaaaa tcaagcttcg catttgtcca cttttcacca tcaacataat
     6241 caacctttag atgatgaaaa ttaaaaacac tattcaattc ttggcgttct ggttgtgtat
     6301 acttaataca attttcaatc gtcgtcgaag acatttctcc tatagtcatc atgtcagtgt
     6361 taccaaacgt ttgacgattt aattcatgca gaaactcatg cactctagga ccatccgtat
     6421 aaaattcttt acctattttg tcagagtcct taaattcacc tttagaaatt aagttaatga
     6481 catcaaatcg aaaaccatca acgccgaagt ctatccaatg attgactatg cgatataacg
     6541 attgacgtac ttccggatta tcccaattta aatcagcttg actgacatca aataaatgta
     6601 aataatattc atctgtctca gaatcatact tccatgcatt accaccgaat ttagaatacc
     6661 aatttgttgg cggcccgtct tcagatgatc tgaaaaagta ataatctcta taagggttat
     6721 ctttagattt acgggcttct ttaaaccatt catgctccgt cgacgtatga ttaatgacaa
     6781 tatctaacat cactttcaag tctttttgat gcgcaacttt gattaacttt tcaaaatcat
     6841 ccatcgttcc aaagtcttca ttgatttcta aataattgct gatatcatag ccattatcat
     6901 tcatcggtga ttcatacact ggtgttaacc aaatataatc aacacccaat aacttgatat
     6961 aatccaattt ttctataatt ccattgatat ctcctatacc attccccgtc gtatcattaa
     7021 acgacttagg ataaatttga tatacaacgg attttctcca atctatttct ttcgacacac
     7081 aattaccacc ttataacgta ataaaggacg tccaaataac gaacgcccct tttttatttt
     7141 attaatcttc aacaatttct ttcgcttttt gtttactaaa atgagacatc acaattgtta
     7201 gtatacatgg tacaacaata gcaatagctg tcacaataag atatactggc caaaattctt
     7261 tttgaattga aatgaatgct ggcacgccac caacaccaac ttttccaagt acgttattca
     7321 taccaacgat tgcccccaat acacaagacg ttgatatcgc agcgataaat ggatatttca
     7381 gaggtaagtt cacaccgaac atggctggtt cagtaacacc taacatacca gaaatacaag
     7441 atgttaatgc caagccttct tctttaacca ttttacgacg tttatagaca aaccatgctc
     7501 caaatgctgc agagccctga caaatattgg aaatcgcaac aattggccat aaatacgtac
     7561 cgcctaagct gctacccatc aattggaaat ctactgctaa aaacatatgg tgtagtcctg
     7621 taattacaag tggtgcatat aacaatccat atattgctcc gccaagccat cctgcatgtt
     7681 ggaatataaa tgtaacacca gatgtaatac ctgtaccaat caataacgca actggtccaa
     7741 tgataataaa tgctaaaaat ccagtaacta aaagcgctac gggtccaacg accaacattt
     7801 ttatcgaatc gtgaacgact ttatttaatc ctttttcaat tttagctaga acgtaagctg
     7861 caattaaaac tggcaacact tgaccttggt aattcaactg cttaatctct aagccaaata
     7921 agttccacgt cggaatattc ccttttgcca aatcatactg agatactaat tgcggatgca
     7981 ttaaaatcaa acctaagact atgcctagaa tcggactacc accaaataca cgcatactac
     8041 tccaaccaat taatgctggt aagaaaataa atgccgtact cgcaatcaca ttaatgatgt
     8101 ttgaaatatc agcaatttgt ggatacatct caataagtgc ttttggacca aataaacctt
     8161 tcattgtaag taaattattg attcccatta acaaaccagc tgtcacaatc gcaggtaata
     8221 ttggtataaa aatatccccc aacaatttga tcaaacgttg tactggattc cctttttgtg
     8281 cagctgcttg tttcgcttca tctttcgaag cttcttgagc acctgtttca tcaataaact
     8341 gcttatacac ttcatcgact gttcctggac caatgacaat ttgatattga tggtctgctt
     8401 taaactgccc cttgaccaac gcgttattac ttaatgcgtc tttatcaact ttactttcat
     8461 ccttcagcac taaacgtaat cgtgttacac aatgcgttgc agcttcaaga ttttcttttc
     8521 ccccaatagc ggttacgatg gcttttacat cttctctttt tacagccata tatttcactc
     8581 ccttaatgtc ttaaaacgag tataacttgt ctagacaagt tatgtaaagg ctttcaacaa
     8641 aatattttta ttttgtcaca attatctggg gtagccattt cagaaaatca atgagccata
     8701 acgacatatg tagcaataac tcctcgctac aattgcgtta ttaacaatac ctcaccacgc
     8761 tctcaccatt ataaagtgta gatgcatggc caactcctaa attgaaaaat gcatatctat
     8821 aaatagcaaa cttgtccaca caagccatcc atcatatttc acaaaacaaa aaagacattt
     8881 agctatgtat attgcacaag ccaaatgccc ttccaatctc aatgccttat catttctatt
     8941 atcaaaaatt acgagccatt caaagacaaa taaaaaaggt aaatactcaa aatttgagca
     9001 cttaccttca ttatatatag aatttaatat aaatattgat ggcggaggaa gagggattcg
     9061 aacccccgcg agccgttaag cccctgtcgg ttttcaagac cgatcccttc agccggactt
     9121 gggtattcct cctaaacaca atatttatca tatatgaacg ttctgtaaaa gtcaatattg
     9181 tttttaagca ttacatgatt accacaaatc attgtcttcc actacagttg acctataaac
     9241 gaatgtaaaa aatcactact tataattaat ctatttgttg tcttacaaac ctttgttttt
     9301 aggttacaaa gatggcatgt gacgttgata gatactagat tttttcaaac tcgattcagc
     9361 atgtcacaac gtcaacgtaa ccaccatttc catacaaaga ttatacacaa actttactag
     9421 ctaaatactg atctactgct ttcgctacgc ctctaccttc tttaattgcc catacaacta
     9481 aactttgacc acgtctagca tctccagcag caaatacctt ttcattatta gtttgatagt
     9541 ttgtatcatc cgccacgatt cgatttctat ccgttttaat gttaaaagca ttcggtactg
     9601 ttggttctgt accttcgaag ccgattgata ataatacaag gtcagcaggc caaaatcttt
     9661 caggtccttc tttcatgacc ataccatttt cgccttgctc taaaatttga gtatacaaac
     9721 cacgtatgtg tcctttatcg tcaacatcgt aacgcattgt ttgaacacca tatgcacgtg
     9781 gttccttacc aaacttagct tcgtactctt ggtgcgcata gtccatttta aacaccggca
     9841 ttgctaaagg ccatgatgca ttttctgtaa atgtaattgc ttctggcaat ttcgtatatt
     9901 tattaaattg aacaatcgat ttacaatttt ctcttaatgc tgtcgctaca cagtctgccc
     9961 ctgtatcacc agcaccaatg ataatgacat tcttatcttt tgcagttatt gttatatcat
    10021 caatttctcc atttaacaac tgcgtttgtt cagttaaata atccatagcg aaatgtatac
    10081 catcacccat gcgtccttct aaaggtaaat ctctaccttt ttgtgcacca gtacataata
    10141 taatggcatc atactcagat tctaacgttg ctttatcaat atcaacgccg acttcaacac
    10201 cattaatgaa agtaatgccc gcttcttcca ttaacttaat acgacgtcga accacatctt
    10261 tatcaagttt catattcgga ataccacaca ttaataaacc gcctgattct ctagcacgtt
    10321 cataaatagt tacttgatat cctagtagat taagttcttc agcagcagct aatcctgctg
    10381 gaccgcttcc aacgattgcc actttttcat ctctacggcg actcggaact ttcggcgcta
    10441 cccaaccatt ttcaaaagct tcatcaataa ttgtgcgttc aataccttta atcgcaatcg
    10501 attctctatt aatcttcatc acacaagcac tttcgcatgg tgcaggacat acacgccctg
    10561 taaagtcagg aaagttattt gtttcgctta agcgttcata agcagtttta aaatcttgat
    10621 gatacactaa gtcgttccat tcaggaatgt agtttccaat tggacaacct attgtttccc
    10681 taccatactg ttgtccggtt tgacaaaacg gcgttccaca atccatacat cgtgcacctt
    10741 ggatagaggc atcttcttta gtaaatcgtt gttgatatgc tttatgatgc ttcaaacggt
    10801 ctaccagtga taattcacct aagtactgtt tgtcatactt cataaatcct ttaaattcac
    10861 ccatcgtatc tccccctttc cttaatacac aacggctggt ttatgtttag catcgattgt
    10921 tttactgtca tcgtaaaatg cagctaacat cgcttcatct tcattgtcat gtaatgattt
    10981 gtgcaaatga attttttgca tcattaattg ataatcttta ggaataactt taacgacgac
    11041 atcttcaatg cgatcaaaat gttttaacac atgaatcgct ctcgtactat tcgtgtgtga
    11101 cacatgttct tccagcattt gcttaatgaa tgctttttct tcttggtgtt taatctttgt
    11161 aaacgaaagc gtatctagtt gattattttc aacaaaagct tctacatcag acgggataac
    11221 gtaagcaata ccaccactca taccttgacc gaagttctta cctacatcac ctaaattaat
    11281 gacatgtcca ccagtcatat actctaatcc atggtcgccg ataccttcaa cgacaacatc
    11341 tacaccacta tttctaatac agaatctttc tcctgcacta ccgttaataa atgccttacc
    11401 acttgtcgca ccatagaatg agacgttacc agcaataatt tcattttgtc gttcttcaaa
    11461 aggtgctttg acaatgaccg taccaccaga taatccttta ccaacatagt cattcgcatc
    11521 tccagtatga tgaatcatta agcctttcgg tgcatatgct gcaagacttt gaccagcatg
    11581 accattcgta taaacattaa ttgtattttc aggaagtcct gcttctccat attgtttcga
    11641 aatctcacta cctgtaataa cccctacatc acgttgttca ttatttactg taaagctacc
    11701 tgtatagcga cgcccttcag caatatatgg cttcgttact tcatataaat ttgttaaatc
    11761 aaatccatgc tcaagattat gattttgttg aatttctttt gtgtttggcc catcgaaagg
    11821 acataacagt ttttcaacat caatactagc cgctttgcta ttcgctttta atgttgatga
    11881 tcgttgtaat aaatcagttc ttccaactaa gtcttctaca cgtttcaaac ctaaagatgc
    11941 taaaatttct cttaattctt gtgcaataaa atgcataaaa ttaacaacat gatgtgcttt
    12001 acctctatat aaagcacgta aatctttgtt ttgagttgca actcctactg gacatgtatc
    12061 tttatggcat acacgcatca taatacagcc caacaccact aatggtgcag ttgcaaatcc
    12121 aaattcttcc gctccaagcg cacatgcgta cgctacatct ttaccagtta ataacttacc
    12181 gtctgtttct aacttaacac gacttcttaa gtcatttagt tttaatgttt gatgtgtttc
    12241 tgctaaacca atctcccaag gaacaccggc atgctgaata ctcgttttgg gtgaagcccc
    12301 tgtaccacca tcgtaaccac tgatgacaat tttatctgca aatgcttttg ccaccccaga
    12361 tgcaatggta ccaacacctg ttttcgaaac taattttacc gcgatatctg catctttatt
    12421 cgcatttttc aaatcatgta tcagttgcgc taaatcttct attgaataaa tatcatgatg
    12481 tggcggtggt gaaatcagac cgatacctgg cgttgaccct cttgtcttcg caatccacgg
    12541 atatacctta gtaccaggta attgaccacc ttcaccaggc tttgcacctt gcgcaacttt
    12601 aatttgaatt tctttggcat gttgtaaata atcactagtt acaccaaaac gcccagaagc
    12661 aacttgttta atcgcactta ctttgttgct tccatcaact tgtacttcat aacgttttgc
    12721 atcttcgcca ccttcaccac tattactctt tccacctaat tggttcatgg cttgtgctaa
    12781 cgtttcatgt gcttccgctg aaatcgatcc ataactcatc gcccctgtat taaagcgttt
    12841 gacaatgtca cttaccggtt caacttggtc gatgtcaatc ggtgtacatg ctttaaattc
    12901 aagtaaatgt ctaatgtgat ctgttctatt tttgttcacc gcttcagagt atgctttaaa
    12961 ttgcgcatag tcattttctt tacatgcgtg ctgcaataag aaaatagatt ccggattaaa
    13021 agcatgatgt tgaccttgtt gtctccattg gaatgtacta cctgatgcaa gataattatc
    13081 atcactttgt tgacgtgctt tattttcagc atcaatttga tcaatcgaaa taccagataa
    13141 cttagactgt gtcccagtaa aataacgatc aatcacatca tgagacaagc caatcgcttc
    13201 aaatatttgt gccccttgat aactttgcac tgtcgaaatt cccatcttag ccattacttt
    13261 aatgacacct tctgacaata catccgtata tgtcttaaca ttatcgacaa cggtgccttg
    13321 taacccttct gtcaatgtca gttgttcaac tgtacgttgc gctaggtatg gcacaattgc
    13381 attcgcgcca tatgcgagta aacaagcaac atgatgcact tctcgtgtct caccagattt
    13441 agcgactaaa cttgtagaca tacgtaaatc tgctttaata agtaattgat gcacatgact
    13501 tattgcgagt aacatcggca ttgcaaagcc attgctatca actaatccac tatcatctaa
    13561 cactagaatt tgagcgcctt gctttacagc attcactgct tctcggccta atgcttctaa
    13621 cgcatcttcc aaatcccctt catataccgt tgataaataa gttaatttaa aatgttcctg
    13681 atcaatcgct gctaagtgtg attcattcaa taccggcctt ttcaattgaa tacgatctaa
    13741 aaccgtttcg tcaggtgcta gtaagttacc ttcgccacct aaataagaaa gttcactcgt
    13801 tacgattttt tcacgatacg catcaattgg tggattcgta acttgtgcaa acagctgttt
    13861 aaagtaatta aatagtgatt ctggtcgctc gttcaacact gcaattggcg catcatatcc
    13921 cattgcaccg ataggatcct tcttaccttc tacaagttcc tgaatatact tatgaatctc
    13981 ttctttcgtg tatgcaaact gacgttgtaa tttaaataac gtctcatctt tccattgcga
    14041 atcttgatat tgtatatttt caaaatcaaa gtcaacttta tggttatcaa tccacgcttt
    14101 atatggtaat tctccagcaa tcgcaccttt taaatcatta ttttcaatga ctttattctg
    14161 tttaaaatca acaagcaata actttccagg attcaattga cctttaaaag caacattact
    14221 ttcaggtacg tccacaacac ccacttcaga tgaaaagaca ataaagttat ctttagtaat
    14281 cgtataacga cctggacgta atccatttct atctgtaagc gcgccaagtt tgtcaccgtt
    14341 acagaacgaa atcattgtag gaccatccca cggttccatt aaataactat aaaattcata
    14401 aaacgcacgt acatttgcat cattcgcttc attatataac caaggttcag gtatgagtaa
    14461 catcgctgcc ttttctggct ccatggctaa cgataagaac tctagcgcat tatctacaat
    14521 ggcagagtca ctaccatcct catcgacaat ttgaaacact ttatgttgat cctcgccaaa
    14581 taatgtttcg attaatttat gttggcgtgc tcgcatccag tttacattac ctttaatcgt
    14641 gttaatctca ccattatgca ttaacatacg gttaggatgt gcccttttcc aactcgggaa
    14701 tgtattcgta ctaaatctcg aatgcactaa ccctagcttt gattgatata aatcatccga
    14761 taaatctgta tatagttttt taatttggtc tgatcgtaac caacctttat atacaattgt
    14821 tttgcgtgat aagctcgtaa aatacaattc taaatcgcac tgagtcgaat agaactctaa
    14881 ttgttttctc gctaaaaaca aacgcttttc aacatcttca atgtccctaa tatcaataaa
    14941 cacttgttga atgactggca tcgtatctgc tacatgttta gcaatggcat ctttattaac
    15001 tggtacatta cgataaccaa gaattgataa cccttcgcct tcaaaatatt ttttaaaaac
    15061 tacttcatgt tcagaaccta aaatgcgttc tttggaaaaa aataacccca cggcatattc
    15121 accttcacct gggatatcaa agtccgttac atgttgtttg aaaaatgcaa aaggtatttc
    15181 agtcataata cctgcgccat caccagtgat gccatctgcg ccgaccccgc ccctgtgatc
    15241 taagcgtcgc aacatttcaa gcgatttatc aatgatgtcg tgagaccttt tattatccat
    15301 attcgcataa aaaccaatac cacacgcatc atgttcctca cgatagtcat ataagccttt
    15361 aattaatttc tcattgtgca tgatgcacac ccctttttta gaatattctg atattagtta
    15421 ttaatgataa ttactataat ctaaaaaatt tatcttttca atatataaaa tagaagatat
    15481 tcatctaaaa aatagaacat ttatcaataa tattgctata atagttacaa aacactcatt
    15541 tttggaggtg cctatatcgc tatggaaatt aaacaattaa gatattttat cgaggtcgct
    15601 aaaagagaac atatctccga aacagcactt gaactaaaca ttgctcaatc agcgataagc
    15661 cgtcaaatta cattattaga acaagaactt aatgtgtctt tatttaaaaa gcaaggacgc
    15721 aatattacgc tcacatctga agggaaatta ttatttaatg aagctctgcg tattattgaa
    15781 catttagata gcacgattga acaatttcaa agccatggat taactaaaaa taaaagtatc
    15841 tacattggct atgatgagag tgacgtatcc cacatgttac taccacttat ccaaacgttt
    15901 catttacaaa atgatacaca tgtcataccg agcttattag aacatgacac aattataaat
    15961 agcgtattaa atgggaatat agacattggt ttcacggaac ttacaccaga aatacgtaaa
    16021 cacaaacaat tacatatgct acctttattc gaagagcact atcatttata tgcgccttca
    16081 gacgatccaa ttacgatggc gacacaccca ccattaattc agtttgagca tagtcatatt
    16141 tattgcttgg caccatttgc cgaaactgta aaaaaacaat tgcgcaagat tactaaatca
    16201 gatgtataca caatcagtag ccaaccactt gcgcagtatc tccttcgtca aaaagaaggt
    16261 tacatcattt catcacaaaa tatacattta ccagaatcta aatcatggat agatataaaa
    16321 ttagatcata ctgaattaaa aagaacgata tgtgctatta caaaggagcc ttatacaaag
    16381 agtgatattg gcatattatt aacacttatt cagcaattaa tgactaaaac gtctacattc
    16441 cattaagtaa ttcgatgcta tcaatgatga ttttcgtttt acacttagca gtaatatctt
    16501 tgaaaataaa aaattaaggg cgtataatca ccatattaat tcattcatag aattggggtt
    16561 ttgtttttga aatatagaaa tttatttaca aagccattta atataatttt aggtttgttc
    16621 tgcatcgcat tcattggtgc tattttattt acattcaata atgagaactt ttacaacaaa
    16681 ccaattggtc aaattataga tgtcaaacat gtttcttcga cacctacaaa agatgcacaa
    16741 aataatcgtg atattaaata taaaaatcaa ttaaaagtca aaatattaaa tggtcaattt
    16801 gctggagaaa cgacgacgat taatcatcag tacgtcaaat cgcaagcaga ttctgaggca
    16861 tttcgcacac acgaaaaagt attattacat atttctaaca agccgagtga tgcttatatt
    16921 atagaaaaga agcgtgatac cttaacggtt atcatcactg gtttgttctt attaactgta
    16981 ttattggttg gtcgcaaagt cggactgcaa tcgattttat cgttaatctt gaattctatt
    17041 gccatattaa tcgcaattta cattcatatt caacatagca atattaattt attcttactc
    17101 atgaccattg ccatgatctg ttcaacaata ctcactttat tgctcgttac aggctggcat
    17161 atgcgtacac tcattaccat agccagtaca attatcggaa cttttttgag tataggttta
    17221 acagaattaa tcatatatat ggcagatggt aaaggcatta aatatgaaac gatgaacttt
    17281 ttatcattac caccgaaaga tatcttccta gcctcagtgt taataggttc acttggcgct
    17341 attatggatg ttgcaattac aattgctagt ggtatgcatg aaattttaca gcgtacgcca
    17401 cacatttcaa tgaggagatg ggcgcttgct gggcgaaaca tcggccaaga tattatggga
    17461 acaatgacta acatcttatt attctcatat ttatccggtg cactacctat gtttttaatc
    17521 tttttaaaaa atgccaatac agtgacttat actatttcga tgaattggtc gttagaaatt
    17581 gcgcgcgcac taacaggtgg tataggtatc gtacttacaa ttcctatcac aatattgttt
    17641 atggaaatat ttgaaacatt aaggagggcg aaacaatgaa tgctgtattt atattggcgt
    17701 tattgctcct ctttctgatg ataattttcg gaggtaaaaa gggattcatc tcttatttaa
    17761 ccctattttt aaatttcgct attttaatta ttagtattgt attgattatt tttggcgtac
    17821 cgatttatgt cgtcacattc tttttctgca ttattattgg cgcttgtaat ttattcgtac
    17881 tcaatagtta caatgttaaa acacaagctg catttatcag cactatcgtt acaacaatat
    17941 tgttgattac actcatttat ttatcggtct acttcggtca tctacaaggc ttttcaaccg
    18001 aacaacaaga tgaaacatat gtgtattcaa tgaatattgg cattgatatg gttcaattta
    18061 tggtgttcac tattattctt gctgttattg cagctgtgat agatttaaca attaccatta
    18121 gttcacccat ttatgaattg catcaagtga atccaacgct tacgcaatac gaattattcc
    18181 aatctggcat gcgtgttggt agagaaattt tagcgacatc tgcgaacacg atttaccttg
    18241 ccttttttgg tggccaactt gctttattta tttggttttt caaacttaaa tattcatttg
    18301 gtcatattat aaactcgaaa atatttgccc aagaatttat ttctattatt ttaggcggta
    18361 ttgccgttgc aatcagtatt ccgattacgg cttggattac ggcattttta ataaaaagac
    18421 catctcgaca gcgcaaaaat gatgagtcta tcaattaatc gcaactagca atataactta
    18481 agttaagcga cctgtggata tatgtcaatt gttcactagg tcgcttttta ctatagtaaa
    18541 ttgtccgatg atctataaca aaaagcacct ttattcatca ttaattgttg tggtcgacga
    18601 tgagtaaaga tgcttttaaa tttaatttat tttatagtta acgccataat atgaacaggt
    18661 cttccgacga acatactatc tcccatatat tgatatcctt gttggcgata aagtgccatg
    18721 gcatgtggat tgtcagtatt aaccgttaac acaatctcat taatatcctg aaatgttgaa
    18781 gtgataaatg acgccaattt ttccattacc actttaccta ttcctctatt acgataacgt
    18841 tgatcaacac taaatgacct gaaaaatact gcatcttggt tatcgctaaa tggtgcgacc
    18901 ccttttccac gatgtaatgt aaaaaaggcc acacattgcc tgccatccat tacaacgatg
    18961 ggataccttt cattatcaga cattgcagct gtaatatttt cctctggcgt tttaacaaac
    19021 ttcaaatcac tttcacttaa ttgaaaagct tctaacatcg aataatcttg tgggacataa
    19081 tgtcgtaaca tgatggtgtc attatttgtc atgtttacca ccataagttt caatccaaac
    19141 tttgacagca ggcgcaatca acgcatcatt atctttatca atccagcgta tatccgtaat
    19201 ttcattattg atagttacgt tttcccaatc gattttggtt aatgcgcgaa atccatttaa
    19261 ctcagttaac atatcctgtt gtggatatgc aggacctaca attgtcccga catattcaat
    19321 ttcatccatt gttaatgtta aatttaattc ttcttttact tctcttaaca gcgcgtgtac
    19381 ttgtgattcc ccttcttcta ttttaccacc cgggaaataa tacttttcgc gattccttac
    19441 ttgaacaagc aatattttat cagctgtttc ttcaactaaa cagacacatt taatcatctt
    19501 tgacacccca actttgtgaa atcaattttt caaattatac tgtacaatta tgttatcata
    19561 tatgagtagt tatagcgcaa aacgttagca attcagcgca cccaactttt catataaaca
    19621 gaagatacta gggggaatta ttattatggc aaaacgttcc aaatcacaac gtttatcaag
    19681 tttactaaat gtcgcaggtt tcatagtcga cggctacaat ggctataaat atcatgctaa
    19741 aaataaaaaa ttagtatatc tttcattagg tttaagcact gtaggaaccg tgttagactt
    19801 ttacatttca attaagtcac cacgtaagtt caaaaaagca gtggcagttg ttactttaat
    19861 aacaaacggt gctagattat ttacaagcat tcgcaaagta aaacatgaat actaattcag
    19921 aaaaggattg gtcgaacata gaacatgaag ttcattcgac caatcctttt tcatatacaa
    19981 aaattcctat tacaacaatc acgactttga tagccccgcc ataaaattta ggattcaatc
    20041 caacttttca gcttgtgaaa tgtaatagga attcattata tatttatata cgtaagactt
    20101 tagtgaatat atctataatt atttacttgg taagctggta ccgttctgta agttaaaata
    20161 cctggtgcag ctgaatagtt catttctgaa actaaaatac taccatcgtt atttacacgt
    20221 tctacaaaca taacatgacc atagtaacct acatctgttt gagcgataga acctacagta
    20281 ggtctattgt cgatagtgta accatctgct gccgctgcgt tatcccagtt attagcattc
    20341 caccaataag tactaatacc tttaccaatt tcagcacgac gattaaatac atgatatgta
    20401 cattgacccc atgtatataa gttttggtga ctgaatactg gtgtattgta acctctattt
    20461 gtcgttgttg cagatcctga gttcgtagat gcattaccag ttactttcaa tttttgaccc
    20521 ggatatataa agaaattatt taagccattt aagctcataa ttttttgata agttgtacca
    20581 tattttgatg caattaatga caatgagtca cctgcttgta ctgtatagta tgatccgcca
    20641 cctgagttcg ttgatggacg gctactattg ctcgcagcgt tacttgagct agcagtacct
    20701 gatactttta atttttgacc tggataaata aagaaattat ttaaaccatt aagtcgcata
    20761 atgttttggt aagttgtacc atattttgat gcgattaatg ataatgagtc gcctgcttgt
    20821 actgtgtagt atgatccgcc acctgagttc gttgatggac ggctactatt actcgtagaa
    20881 ttacttgagc cagatacttt tagtacttgg tttgggaaaa ttagattaga tgttaaattg
    20941 tttaatgact ttaatttagc aatcgaaatc ccatacttat ttgaaattgc ccacactgat
    21001 tcacccggtt ttactgtgtg agttgtagcc gcatttgctt gagttgccgc aacagcgcta
    21061 atcgcgcttg tcccaataat agctgcaatt actttttttt gcactttaaa atcctcctct
    21121 tgcttaactt tcctaacatt cttttatccg aatttatgaa tactacatca ttatacgatt
    21181 ttattatgta taataggttg atgtttgatg acattatgat tacaaaaaaa tcatatactg
    21241 tatcatcaaa ttttataatt accccttaaa attattacaa cttattagat tttacaatat
    21301 ctaaattatt acaatttcat aatatttcac tataaaatga ttacaatccc tttctctatt
    21361 ggaaataatt ttattctcca acaataacgc cctacaaaca taagcatgaa ctttgcttgt
    21421 aggacgcaat ataatttatt ttgctaatga cgtttctatt gccttaatct catctttaga
    21481 taaattaaca ggtttctctc catctttgac atcttcctgc aacgcttttt gagcttcttt
    21541 tgaatgatac aattctacga ttttagcata tgttttgtta tccaagtctt tgtcattaac
    21601 tgcaacaata ttaatatatg gctttactgc atctgaattt gatttttcta aaaatatcgg
    21661 atcattttta ggatctttac ccgctttagt tgctacaccg ttattaataa ctgcaatatc
    21721 gacatcagat aaagcacgtg cagtttgttg tgcatctact gcagtaattt ttaaatgttt
    21781 tggatttgac gttatatctt tcaccgtgcc tgctaatccg aaatcttttt tcagttttat
    21841 taaaccagct gcttctaata gtttaagtgc acgtgcttgg tttgacacat catttggaat
    21901 gacaacttta gcaccatctt taaccttttt gacatcttta attttatctg agtaaatgcc
    21961 caacggtgct aaaactgttg tacttaatgc tgaaatcttt gttcctttat gcgccttttt
    22021 atattgatct aaaaatgcaa aatgttggaa tgcattcata tcaatatcac catcatttaa
    22081 tgctttattc ggtaaattgt aatctgagaa gtgcttaatc tccacatcaa tatcatcttt
    22141 tttagctaat tctttaacct tctcccaagc cttagtgtca tttgatgcga caccaattgt
    22201 tactttttta tcattgttac caccacacgc tgctaataat acaagtgcta cgataactaa
    22261 cccaatcaat cttttcattc tatcaattcc tttcaaaatc ttcactatat atcattaatg
    22321 tctacgtatg aatctagcta gaacattccc tagcgtttga atcacttgga caataatgac
    22381 taatacaata acggtaataa taatgaccgt cgtatcaaat ctttgataac catacactaa
    22441 agctaagtct cctataccac caccgccaac agctcctgcc atcgccgtac ttccaataag
    22501 tccaataatc gcagtggtaa ttgctaatac taacgaacct aaagcttcag gaattaaaaa
    22561 atatctaatg atttgtagtg gtgaagcgcc catcgctttc gccgcttcaa taatcccctc
    22621 gtctacttcc aataatgagt tttcaacaag tcttgcaatg taaggtgcca catatactgt
    22681 taaaggcacg atggcagcag tcgtaccaat tgaagtacct actactaatt ttgtgaatgg
    22741 cacaatcgca attaacaaaa taataaatgg tagtgacctt aaaatattga ttaaaggatt
    22801 taaaacttga tgtatcacta tattgggcca tatgccttgt tttcgagtaa ttaccaataa
    22861 gacacctaat ggaataccaa tcactgctcc taaaaataaa gcaatagata ccatatatag
    22921 cgtttcgtac aatgcttgta ataactgtgc actgtctaaa tcagaaccaa acatatgtta
    22981 atgcacctcc tcaaattgaa tatttttctc tttgaaatat tgatttattg ccgtgtcttc
    23041 aaattgttga tccatattaa atcgaagcca catataacat acggtgttac cttgtatttc
    23101 tgacatagat gaaaataaaa ttttaacctc tctgccacaa atttgaatca agtcatttat
    23161 aatcggttgt gtcacctgag tttcctcgac gaagatttta taatctttaa aatcgccaac
    23221 ttgttcgtca ttcaatcgac gaatcaatga tgtacttggc tcagtctgta taactgtaga
    23281 cacaaaattt tgagcaatcg tcgttttagg atgactaaac acctctttaa cagttcctgt
    23341 ttcaaccact ttcccctttt ccattacagc aacacgatta caaatgtctt taataacgcg
    23401 catttcatgt gtaatcatca taattgtaat gccaaaggtt tgattgacat tctttaataa
    23461 cgtcaatatc gaagcagtcg ttgctggatc caatgcgctt gttgcttcat cgcataggag
    23521 tattttcgga ttagtaacaa gcgctcttgc aatagccacc ctttgcttct gcccaccaga
    23581 taattcatca ggaaattggt cttttttatc actcaatcct acaaattcaa gcatttccgt
    23641 tactcgttgc ttaatttctg ttttgctttt cttacttaaa atgagtggca ttgctacatt
    23701 tttaaatacg gtagctgaat ttaataaatt gaaatgctga aatatcatac cgatatcttt
    23761 cttaatatcc ctcatcattt tatcgctata attcgtaata tcatgtccat ctacaatcac
    23821 ttgtccattc gaggcagctt caagatgatt cacgagtctt accaacgtac tttttcctgc
    23881 accactatat ccaatcacac caaaaatatc attgcgattg accgtaaatg atacgtcctt
    23941 caaagcatct attttttgct tctttttatt aaaggtctta cttacctgtc gaaactcaat
    24001 catcatcgcc cctcctacaa attcttatta atccactagg aataagagca ttatatgtaa
    24061 aattgcatat atcgtcaata caatttgccg aattttctaa aaaattaaaa aataagtaat
    24121 tcatgtgaca atgacgaatt gtgagactac tatgacattt atcaaattaa atccataaaa
    24181 atgtccacca atcctccaca acgcaattac taaatattaa catcgcacaa aaaagcacta
    24241 gcatattcaa gaacaacaaa cgttgaactc aaaatatatg ccagtgctgc tattatttat
    24301 aaagtatcta gtgcttgttt taaatcatcg actaaatctt cagtatcttc aatacctaca
    24361 gaaattctta caagtccgtc tgtaatacct tctttagctc gaatatctgc tggaatggat
    24421 gcatgtgtca tcaatgcagg tactgaaatt aaactttcca ctgcacctaa actttcagct
    24481 aatgtgtaat acgatgttgc tttaatcaat tgtttggcac tttctgtatt tttcacttca
    24541 aatgcaatca cacctgtatg gccatccgct tgagccatat ggacatcatg atttaaatga
    24601 ctttcaatac ttggatggaa cacttgttgc acagctggat gtgcttgtaa cattttaata
    24661 atttcaataa cgctgcgatt aatttgttcc atacgtaaac ctaatgtttt aatacccctc
    24721 acaagtaaat agctatcttg aggtcctaaa atgccacctg ttgaatttga aataaatgct
    24781 aaacgttctg caagcttgtc atccgatgtt gcaactaaac cagcaacgac atcactatgt
    24841 ccacctaaat atttcgttgc agaatgtaag acaatatcga tacctaaatc taatggattc
    24901 tgataataag gtgtcataaa tgtgttatca acaactgaaa tcaaaccgtg ttctttcgca
    24961 atttcagcag actttttaat gtcagtaaca cgtaataatg gattagaagg tgtttcaata
    25021 aacaacatct ttgttgttgg gcgtatcgct tgtacaattg aatctgtatg cgttgtatct
    25081 acaaaatcca cttcaatgcc aaatcgtgta aatacttttg tcaatgcgcg ataagtaccg
    25141 ccgtatacat ctgaatttaa aataatatga tctcctttgt ccaacagcat aacaactgca
    25201 ctgattgctg caacacctga actaaatgca aagccatgtt tgccattttc taatgtcgca
    25261 ataacgcttt ctacagaact tcttgttgga ttcgcagtac gagaatattc atatccttga
    25321 cgtaaatcac caatatcatc ttgtaaatat gtacttgttt gataaattgg tgttgtaacg
    25381 gcacctgtat aatcgtctgt tgtgtgccca ccatgaatta atttagtttt cttgttcatt
    25441 attattctcc tcataattaa atatttgctt agacatatat cgatcactac catctggaaa
    25501 tacgacaaca atcgtacctt cagataattg cgcttttaaa ttcaatgcac cttgtaatgc
    25561 tgcacctgaa gaactgccta ctaacaaccc ttcatttata gccaaacttt tgacatttcg
    25621 aaaggcatct tgatctttaa tcgtaaatat cccatctaca agacgtctct ctaaaaatat
    25681 cggccatttc tcagaaccga taccttcagt gtcatgtgca tgagctggcc ctccatttaa
    25741 cacggaccct tctggctcaa cggcataaca ttgcacgtga tgttgcttta aataacgtgc
    25801 ggtacctgta aatgtaccgc cagagccaat accagccaca aaataatcaa tttgctgtaa
    25861 tgctgaagtc aattcgggtc ccaatgtatg aaaatatgta tccggattat gttcggattc
    25921 aaattgattc atataaacgg caccatattt ttcagcatag gaacgtgcag ctaattgtgc
    25981 cccatgcata ccttcagact gactcgtcct tgaaacttct gcaccaagcg ctatcataat
    26041 attaatcttt tcttctgaaa aaccatacgg cgcaaagatc ttacatttca aatgatgtct
    26101 attcgctgca atagctaacc ctatgcctgt attaccagca gtcgcttcaa caatagtttg
    26161 acctgcacgc acacgccctt cttgaattgc cttctctact aaatatttcc cgagtctgtc
    26221 tttaacactg cctccaggat tccattgttc aagcttggca taaattttaa ctttatcatc
    26281 actataatgt tctaacagta ctaatggtgt attgccaatt aaatcataag taatcataga
    26341 tgcaccctca tctgacatgc cgatcaaatg aatgaaacct ttcttcatgt ctcaatttta
    26401 attcttactt ttcagataag aattataaac gacattttgt tattttgcaa ttatctaagt
    26461 ttcgattaat tcagaaccag tactaaattt tcaattccaa acaaaaaaac acctgagcaa
    26521 cacaaatact tgtgtgtcag atgcttctat atattaacta aataattgca cgataaagac
    26581 taaaataata acgacaggca tcgcatactt aattaagtaa taccaaccac tgaataatct
    26641 aaatcgatct ttaccaaaat attgttgtaa taatttttta tctaataatt gtcctacgac
    26701 aagcgtagta cctaatgcgc ctaatggcat caatacattc gaaacgatga aatccatatt
    26761 atcaaaaatc gttcccgcac cgaatcttac atcttttaag ataccaaaag ataaggttgc
    26821 tggaatacta atgataaata ctaaaatact accgatcact gcgacttttt tacgttttgt
    26881 attgtcattc ttcgtgaagt tagaaacatt taattctaat aaagaaatag atgacgttaa
    26941 agccgcaaat aagaacagca ctaagaatcc caaatagaat aatgtgccta gatgcatttg
    27001 actaaagacc attggcagta ctttaaataa taatccaggc ccttcttgtg gttcatagcc
    27061 aaaactatgt aaagccggaa atatagctag acctgccaat acagatacaa agatattcat
    27121 aacaacgata gaaatagctg atgacttaat cgtcatgtct ttagaggcat aactcgcata
    27181 agtaatcata cctgtagttc ctaatgataa cgtaaagaat gattgaccta gcgcaaacaa
    27241 gatgccatca gcagtaatct ctgatactct tggttgtaaa ataaatttca caccttctaa
    27301 ggcgccatct aatgttaaag acttaatcac aatgacgatt aaaaagacaa acagcaatgg
    27361 catcataact ttcgatgcct tttctaatcc tttttcaaca cctaacatga caataatcat
    27421 cgtagcgaat atgaatatac cttgccctag aacggttaac caaggatttg atattaccgc
    27481 ttcaaaattc atttcttgga gatgattgat gcgttgaaat ataactaatt gccataatac
    27541 ttgtccgatg taaatgacaa tccaaccacc gataacacta tagaagccaa ataaaataaa
    27601 cactgccaaa ttaccgttcc agccaatgat attgagccat tttttaccag ttaatttact
    27661 atatatttgt gttgtatatg tccgtcccat tttcccaaca gtgaattcca taatgagtaa
    27721 tggcaaccca acaaaaatgg tgaatattaa gaacatagct agaaaggcac cgccgccata
    27781 aatccctgcc atatatggga atttccacat ggcaccaaga ccgattgcag aacccgcact
    27841 agctaaaata aatccagttg atgacttcca ttgtgattgt tgtcttttca tcattcacat
    27901 ctcctttaaa gcgttaaagc gtctacgtgt ttaagtaaca ctctaacatg tatgtaaata
    27961 acatgcaata cacctgcatc aaaattgttt cattaatacg gtagcacctg gatttatcat
    28021 aatataatca actcttgtaa tgtgttgtca ttacgctaca ttaaattttc tggttcaacg
    28081 ctgtatacgt gcgccaatat ctcgccacat acaacaccat ctatatcccc aacatctcgc
    28141 cacataacaa cttcaggtgc aatgcccata aaatttccac gacattacaa tacaaatcaa
    28201 cttctggtat aactccataa tatttccacg acatcacact acaaattaac tttagctaag
    28261 ttccctatac tatcacgcca caaaaaacac tcaaatgaca aatataataa catcgtcact
    28321 caagtgttaa tttttaaaat cttcaattat gttaaagcat tgaaaaagcg aataacattt
    28381 tcttctaaaa tatctctgcc ttctccatgc gtcattaaat gatgcgaatc cttcagacca
    28441 tttaattctt tgtctactga tcctaaatgt tcataaataa aatgtgcact tgtttcatag
    28501 gatggcgcat ctttaccacc aaataatata ttagctggca ttttaataac atttaaattt
    28561 gtcatgatat catcaataaa atgttgaaac ttcgtaattt caccttcata atcatcaatt
    28621 gctgctaatt gcgcactcga tgcttgctga tctaaattta aaatattcga catgcgttga
    28681 ctataataaa ctaaatgttc tattaagcca tcgtcactct tttcctttgg tgctgacatg
    28741 acagcgatac gtttcaaagg atagtgttgc gccaatttta atgtcattaa tccacctaaa
    28801 gacacacccg ttgcactgat agattcataa ccttcattga ctaaaaattg gtaagctttc
    28861 tcaacttctt cccaccaatc atctacatta tatgtcatga aatctttcaa caataaacca
    28921 tgacctggat aattcggtgc ataacaacta aatccttggt catttaactc agctgcaaga
    28981 tgcttcacat cccgatttgt acctgtaaat gaatgtaata ataatatcgc atgtccattt
    29041 gtgcctttta aatacgatgg actcggtgtt ttaattctca tttttctata tacctccact
    29101 atgtctaaag atgtttgcta aacgcgttgt cgtcgatgat taataaagta tgggtatacc
    29161 attaagaata acgctaccca aatgattgct agtgacgtgc cgccaatgac atctgaaaag
    29221 taatgtgcat gaaaataaag gcgacaaaat aatatgctaa gccataatat tcccataacc
    29281 aacgcactca acacttttgt tattgtctta gcagcaagtg aaataataat gatcattaag
    29341 gcgaaatata ataatgtgct ggcgttggaa tgtccactcg gaaatgaaaa gcctgtatca
    29401 acggctaaat gattatatgg tcttggacgt aatacagtat ctttaattaa tttgttcatg
    29461 atgacacctg aaaccaaata tgtcacaaac caaaccgcta aatgcctctg tttaataaac
    29521 agtatgattg tgacgataat ggaaatcaac acgacacctt tgacatctcc aatttccgca
    29581 ctaaacgtca tatagtaatt aaacaaattg ttaacatact gacgttgtgg ctcaccgaaa
    29641 taatctgtaa accatgttaa tgatcccata tctatatttt taagccattc ttgatttgtc
    29701 actacactgt aaaacatacc tataaataca atcagcgcga ttaaaaataa aggcactgtc
    29761 attttcggtg atgttaattt tttatctatc atcttacaat ctcctcgtat catcattttc
    29821 attttacaaa tgttatccat aatatcaatg tgccacaaat ttcactttac cgacaatatc
    29881 aaaattataa agttcatatt gttatgtata ttgcaaataa aacattgtat aattgaaata
    29941 acaatatttt gctattttca atttagtacg atttatattt attatacaga gggggtaagg
    30001 cgtatcaata gagttatttt tgtctatata gcgttaatca ttacattagt tagattcttt
    30061 acccctattc atccatcatt ttcaaatttg atttactgga tatttgtatt atattttatt
    30121 cctattatac tatgcgttat cggtttcaag gccgaaaaac ttattgcaac aatggtcatt
    30181 atacctaatt ttttaggaat actttatcga ttatatgcct acgtcacaca tatactcttt
    30241 atgtaaaagg atagtgggca tgtctcgcaa caaatatgct tgcgtcgact tgtcaccatt
    30301 cgcaaatttt atgacatacg cctttcacgg gctatattca gacccacgca ttcatccacg
    30361 taataaacac atcatgtaat agaaaaacag cacacccaaa tatatggcgt tgcgctgttt
    30421 aaccaagcat acttctatag ctttaataag ccagcagaag catacctaac cttcttaaat
    30481 atgcttttcc aaattatcct caagtttgaa tacgataata cgttcacctg taactgtact
    30541 taaatcacta tggaagctca tcactttgat acctgtaatt ttaaaaatga tatcattcaa
    30601 atcttgctca ccggattcaa ctaattcaga acgtgttcgt ttaatattta ataatccttc
    30661 attcgtacta catacacgat attcagctgg cgttaagata ccttgtaaac taataatcac
    30721 catatctctt aaaatgtctg atttaactga caaggaacct ctgccaagga aatctttttc
    30781 ccattgcgta atggctttac tgatttcagc ttcgatttca cctttcgttc ttttcatatc
    30841 actaactcca ataatattaa aattgattac ttcatctttg tatcgttatc acgacatcaa
    30901 ctattacatt aagtttatca tttttagtat attttaagaa gctagaacat tgtagatatg
    30961 atgatatatt agttacttag catcgcaaca tatcatcgtt aaatccaact tttaaaacgc
    31021 ccttcctcat taacgctcat taaacgcagc caatgattag acaccttcct agcgaaatgc
    31081 tcattattcg cgagtagtct tgctacaaca tagtcgggtg cctgaataac gacaagtaaa
    31141 cgaattggcg aatgatacat cgtccgatca gcagccataa cagattgcca tgataagcca
    31201 tacatcagat cactcgcatt accttgcatg acaccaacac ctgacgtgac ggtttgtgtc
    31261 gctttattcc cacttccgta aaaatgcggc gcaactgtcg acgcataata ttgtaaatta
    31321 atccattgtg ccacaagtgc cggaccagaa atgatggtat ttaataatgt gccatcttta
    31381 tctttacgcc aatcataatt gtgtaaaaat gtccgccctt ctaaatcaat gccttttgtt
    31441 aattggcgtc gtccaattat aaatgatgca tttttagcca atccccattc tggacgtacc
    31501 tcactccaat cactcgcaaa ccgctgcgct tcttccactg gatgattcac acgaccaatc
    31561 gttggcagtt tgtccaaacg ttcgcgattc gcgtgttcag aaatcatcgg catcgcgtca
    31621 ttcaatgatt catatgcatc taaagcaata gaagataatg tgtctggcac atatacccat
    31681 gccaacgtat cagtagacgt atgatgttct gctaccgcaa aaacagttgt ctctggaata
    31741 tacacacctg attgttttaa tccttgtctg acatttggac gattacatat catcgctaat
    31801 aacttagcat taaaaccgct tgatgcgcca ccacaagccc cacattcaag tgatgcatga
    31861 tgtggattat tgtgagaatg actagcatga cctgctaaca caacgaacgg cgcaaatgct
    31921 tcggttaaat ccatcaattt caacgcttgt aacgcgaaat caatttgctc ttgctcagta
    31981 aatccaacag gtaagtctga tgttcggtca aactcacgat caatcgtcaa ctttgtttca
    32041 ggctttttca accacttttg ttttattttt tgtaaagacg cgcgactttt tctaggcata
    32101 atcgaattga caatggtact taagcttaaa aatggcccac ttaattcagg caataacaga
    32161 ctaggcatga cattattttt catcaattta aatgtgtaaa acatcgatga cattgtctgt
    32221 tgctgttgtc gataaacatt catatcgtag cggtctgcaa attctttaat gcgatatgcc
    32281 ggcggtacca tgacaggtaa tgaatcatgt ttgaattgtt cgtctacggc atctttttga
    32341 ataggtaatc caaagaagcc tgcaatacca atcgtttcaa agggccctgc tgcttcgata
    32401 tgtctacgaa atggttctga acgaacatct atacaaaatg caatttgcgc tttcgttgat
    32461 gtgcccatct gatttagctc gctattattt tcatcaactg cttgtgtgtc atttaacaat
    32521 actgaatgtg gctgattagc gttatcattt tctgagacat ttacttggtt tacatctaat
    32581 gcgcccgcca cactttcatg acctgcttta attttttgtt ttaactgaga ttcgtatgtc
    32641 atttcccagg caattagcca taaattttta aatacatttt tattcatagt tgctgcaaaa
    32701 tgaataaacg tttgaatttc attgacgtca tgttgtagta atacatcgct aggcatatca
    32761 ctgtagtaac accatgatgc aacagtttgc ttaaaccaat tttccgatct actttcacaa
    32821 tctttagcga ctgacttaaa ctcatcacca actagcaatt gttcgacaac taaccgaatt
    32881 gccaaataat ccgttaacaa atgttgttca aagtaatgct gttgtgaacg gtaatacaac
    32941 atacctgccc aacccggtaa cgccaaaaga tgtccttcaa cataagcttg gtagtcttcc
    33001 tgatctattg aaaaatgagt taatactgac tctatcgtca tttcaggatc attgggtaag
    33061 cctttaatca cttggcgctg tgctttagta aaactatggt catgttgcgc taaatgcaac
    33121 catgcatggt aaaaactttg ctcacgcttc ggcattgtcc aactcgatag aaattgatcg
    33181 atataaagtt tcgtccattt aatcatttga cgattcactt gttcgctaag tggctcacct
    33241 tgttcatcta ttattgcatc actcatcgga cgtacatcat agtgatgata tgattcagcc
    33301 atatcacgtt ttgatttttc taatagtaga tcagcaacaa catcaacatt tgaatgattc
    33361 atatatgatg caggtacgtc ttttaatgtt ttaatgttat caatataaag attgatgtag
    33421 tgttgcggga tattgtagtg atgttcaagt aacatatcag taacaagttg attaaagaca
    33481 ctttcatcta attcaccacg tgccacagcg ctttctatta atgctttatt tgggaaaata
    33541 tccacatctc gaacatcacg taaccatttt gcgacatctt caaacgtatc cgcttctaat
    33601 ccttcccatg gatttcgtgc tgcaaaaatc gaaattggtg ataatggtgt aataacacgt
    33661 ttcgcatttt caatgactga attgatattt aactgtgttg tcataccttt cacctcctat
    33721 aaatacttct tcaaataatt cggatgactt tctatcgctt tcgagcgtgc ttcacctaga
    33781 ttaactaacc acacgtacaa taccgcaaaa gccttagagt atcgatgccg cgccacccaa
    33841 atacttaata aactgccaaa gattaaaata acaacactaa tgatgacact cactgtaggc
    33901 ggcgttgtcg catgtgttgt tatattttgt aatacagcgt aaaaataatt atgtgtgatg
    33961 acgtagataa atgtcacgat tgcaatcaaa atcataccaa caagacgtgc catgcgtcct
    34021 ttactaaagg ctaccatttg attccaagat acaagtaatg accatcctag aatgagtgca
    34081 cttaacactt catatgcact tctgtcacta ctcatccaaa atagaaatgc cacgataata
    34141 gctaatacac gtcccatgac aatccagcca taagcgtctt tagcagatgc ttgttttgga
    34201 atattgaatc gcttcacgat agaacctgat tgtaaaaata atgttgcttt aaaaataccg
    34261 tgcaatatta aatgaataat cgctgctgaa tatacaccca atgcacattg aactaacata
    34321 aagcccattt gactcatcgt agagcccact aattggcgtt tatagtcaac ttgaactaag
    34381 ctaatacccg atcctaacaa tacagaaata ctagaaagga taagtaataa tgataacgca
    34441 aatccattat caaatatcgg cgcaaaacga gttagaataa caccacctgc attcacaatt
    34501 cctgcatgca taattgccga tactggcgtt ggtgccgtta cagattcaat caaccatcga
    34561 tgaaaaggaa attgtgctgc cggtatcatg acagctaata caagtagtac attcgtcaac
    34621 aatgaccatg tcggatgaac tatatgttgt ggtacccgcc actcgccagt cgcaatataa
    34681 atagttacaa ttgctccaac gaatgcaagc caaccacata aaaatgtcat gcttgataat
    34741 ttcgcagact cacgtggcac tttccaaaaa cgattaacgt tcatcagcaa tgttaaacat
    34801 aataatgtaa taccccagca gagtgccatc agtcttaagt cttcagacat ccatgctaaa
    34861 gatgcaaacg acgtaatcgc agtgaacaat ggaaagtaat gtctataatg atgatcacct
    34921 agtaaatatc gcattgaaaa cttttgaata ataaagccaa gcgccattac aaagccagct
    34981 aataaccaag ataaacgatc tattttaaat ggacctaaga catgttgacc atgaataccg
    35041 aaaaagccaa tgactgcaaa taatactggc atgactagta tgtataaatg taatttaata
    35101 tatctcattg gcataactgg tgctaaaaac aacaagccac ttatcaatgc aatgataagc
    35161 gcaataacaa acagtgaaaa tagcaattga aaacttaaca ctgcataacc tccttatttc
    35221 taatctctcg cataattgct tatgtataaa aataaaaacc tacaatagta gattctgtac
    35281 ataatggcag aaaatttact attgcaggtt tcagtttaac tagacactgc atcacggtac
    35341 gttgatatac cttgttgcag tgttctcttt aagcgtgctc ccatgcacat atgtatataa
    35401 aatgttactt ctgtctgttc aattcatctt cataaatatg ctttgcctag acgagaccta
    35461 acgtgttatt cgttttaaac ttataacata aaatataatt aaatttctgc ttcatgtcaa
    35521 attcatgagc ttaacctcta ttaaaccaat gattgtaaag attttgtaaa tgcacctgta
    35581 cagttaggca gtatttcccg tccttttaaa ataaaaaatt cgcagttatg atcataacaa
    35641 ttcaagttag gaaaaaaatc aattacgcac aagataacta tgtacaatga agttaactca
    35701 taagcaaagg aggtaatctt aatgggtatc atcgctggca tcattaaagt tatcaaaagc
    35761 ttaatcgaac aattcactgg taaataagat ttcataacaa acaaaggagg tctttcacat
    35821 gggtatcatt gcaggaatca ttaaattcat taaaggatta attgagaaat tcactggtaa
    35881 gtaagttata aaaatctcat agatatgaac atcttatttg aagggggcca ttcacatgga
    35941 attcgtagca aaattattca aattctttaa agatttactt ggtaaatttt taggtaacaa
    36001 ctaatctcaa acattaacga tcaacaactc atcactatgt taaatcaaca tacaggagga
    36061 caaaacgatg gctattgtag gtactatcat taaaatcatc aaagcaatta tcgacatttt
    36121 cgcaaaataa tttaagcgaa ttgaatactt aaaattctca ggccactata ccaataggga
    36181 tggtggcttt gctttgtgtt gatttattga tgtgaggtga gtcttgttag tttgttgcaa
    36241 ataaatggtc ttggtgtttt ttgtatagga cgttcttagt gggacatacg gaatattcgt
    36301 gatctttgta gtctgacgcg ttatattttt gtggcgtgtt ttatgtttga tactcgagtt
    36361 ctgagacatt catgatttgg catgcgaaat cttaatgatt ttcatgatct agcgcaagat
    36421 atattggcca cgtgcggaat tgcgttgcac gtttagactg aaacactcgt gtgaccgtaa
    36481 gtgttaatag tacattgata gctgcattta cttcactcat ttttatgact gttaaacaat
    36541 gattgtacct tcaattaaca gttggtacga tggttttgcc atttttcatc aacgtaaata
    36601 taaaaaggac taagacacat acatgtccta gccctatgga taaaatgcaa atttctgctt
    36661 tatcaaaact atcacacttt agatagattg aaaacaaaaa gatcctaaga acaccttaac
    36721 tttttattaa ttgtcataaa ttgcaaacaa ttaagccaca attcaaaaat gattatactt
    36781 cattcaactt atcgtgctgg tctaatttgc cattgatatg gatcttcaaa ttgttgccaa
    36841 tctgcatcaa tttcttgcgc attgactaag catgcgtcga gttcttttgt taatttttct
    36901 tcatctaatt ctgtaccaat aatgacaaat tgtgtatgac gatcgccata ttctggatcc
    36961 cattcagctg cgacatcttg acgttctgct aatatttgtg tttgttgcgc ttcagacata
    37021 ctagccaccc aatatgtaac tggatgaata ttgcaagatg accctgcttg agataataaa
    37081 catgctacgt gattgtattg tgctagccat acgatacctt ttgatcgaac gacattattt
    37141 ggcatgcttt ctaaccaatc attgaacctt ttagcatgga aaggtagacg acgtttatat
    37201 acaaacgatg atataccata ttcttctgtt tcaggtgtat gcgatgcatg cccaccagac
    37261 tcaagttctt tgatccatcc tgctgactcg ctcgcttttt caaaatcaaa acgctgcgta
    37321 ttcaagactt cttttaaatc tacttcagaa tttgttgtct taataatttt agcagtcggt
    37381 tgcaatgcgc ttaacatttt ttctaacttc gctagttctt cttcactaat taaatcaatt
    37441 ttattaataa tcaatacatc acaaaattca acttggtcaa ttaataaatc agcaatcgaa
    37501 cgctcatctg tttcgtcaac gctttgatca cgatccatca ataaatcttc tgagttgatg
    37561 tcatgtacga agcggttagc atccacaact gtaaccattg tatctaaacg gcaaatcgct
    37621 gtaagatcaa tgccaagttc atcatcaata tatgagaaag tttgtgcaac aggtactggc
    37681 tctgaaatcc ctgttgactc aataacaatt tgatcgatgc cacctttttt cactaaacgc
    37741 tcaacttctt ttaataaatc gtctctaagt gtacaacaga tacaaccatt agaaagttcg
    37801 actaattttt catctgtacg cgatagtccc ccaccatctg cgacaagatc tttatcgata
    37861 tttacttcac tcatatcatt tacaattacc gcgatacgtc gaccttctcg attttgtaaa
    37921 atatgattta acaacgttgt cttccccgag cctaaataac cacttaatac cgtaactgga
    37981 attttagcca tatatatacc ctcacttcaa tttatttgta aataggaata attccgtttt
    38041 acattatata ggagcgtttc ctctttcgca atcttcgata ataaaaaaat agtatactta
    38101 attaaattat tgagcgcttt actttataat ggagacaaag atatatctca cgaaagagaa
    38161 tcgaggtgta taaacatgtt atttgtcatt ttagttttat atgttactgg tattgcattt
    38221 attctactca gtgtttttgg ttcaaagact gaaggattat ctacgaaaca tactttatat
    38281 accattggca gtgctattat aacgattgct attttcattt caattggcta tgccattcaa
    38341 tacttaactg cagcgcttta tggtttgtaa ggtgaaggtg atgagtaacg ggtagttcgg
    38401 gagaggttaa cttgcgttga ttttgataaa gtgatcatag cttttagtac ttgaggattt
    38461 ttattgttgc tgttacgaat gtggtcatgt ttaatgcggg acagtaattt aagttgtttt
    38521 tttacaattg agagtgtgat atttcgattc ggttcgaatt actttacatg ggaataatat
    38581 aaattaaaaa gaagcggcct agtgtcagtt gtgaatatac tgaacattgg tcgctttatt
    38641 tagtagtatg atatgtagtt tagctattaa tttttttcag gtcatcctta atgctgtcta
    38701 tctcagacat ggcactttta acccaatctc cttgagctgc acctttaaaa ttagctttaa
    38761 aagcttcgca atgttgcgcc atttgttcaa ttaatacttt ttcttcacct tttaatccgt
    38821 tttcaatatc tttgtattta tgcttatgtt caggtgcaat aactgtgcga atattttctt
    38881 tttgcgcttc cattttagat atgagattaa gtgtttctac tgtagtactt atatctggca
    38941 ttcttaaggt catatctggt tctattagag tcatttaatc tcctccaaat tatcagtcac
    39001 ttagcttatc taactgcttt tcataagact tttttaagtc ttctttatat tcttctaatt
    39061 tccattcttg ctttctaatt atctcattga tttcttgctc atacgcttct atatttctcg
    39121 tcattttcct ttgggcatct tcactataat tcatttcgtg cttcaaatat tgaaaaacat
    39181 cataactacg ctcattttct tttcgagttc tatgcctaag ttcatttaaa ttttgcagtt
    39241 cattctctat acgttttttc tcagattcat atgattcttc taattcattt ttattattaa
    39301 tttgattaaa catcatagta atccacctat ttgtgatgca agcacttgat ctttatcaac
    39361 gatttcatta atactgagtc taactctcat aatataattt gatacagaag accctatcgt
    39421 tgaaagttgt ctttcttttg cagatatcat ttgttcaggt tctcttacca tactactttc
    39481 attcacttga ccttcatcta aagcattgag aactccattt acactcaaat cttcacctac
    39541 agcttttcca gctttttgag cgtcttccca attttttcca aatttctttt tcttttcatt
    39601 atacattttt ttgatttgat taatttcatc gtctatcagt tgactgaatg actgcgcaat
    39661 tgttaaagct gttaaacttt ctaaaacttt ttgctgcgaa gaagataatc ctcctccatt
    39721 tgtagtcatc atatttgctc taagtaattc tatactagct aatttagaga tagcattatt
    39781 tgaattcttt acaaatgact tattattttc ttctgcataa ccttgtagtt ttttaagttc
    39841 tttttttata gctttttgtt cttcttcggt cagaaagcct tccatttcat gacctttacc
    39901 atttttaaag actttatcat taccgaaaaa aacataatgc ttatatttct tcagattttc
    39961 tgtcaatgca tcattttctg caacaaacct agtaatatta cctttataat ttttatataa
    40021 ttctaataaa cgttttccat ctggggaatt aggattcaaa ctagttatag aaataggagc
    40081 tggattataa accacaatgt tcggaatacc attactcatt cctagaacta ccgcatctct
    40141 tccacctagt gaatgtccag taataaaatc aatgtcatac ttacttttta ttttatttat
    40201 aaattcttgt gtattcgcat atctatagtt ttgatcagat gcaggagaat ataatatttt
    40261 aagatctgca tatccatctt taactgtttc caaagcattg gtaacatttt gtcttgaaaa
    40321 ttcaccttct tttaactttt taaaggcttc gtcttgtaaa ttagtcccag tcatcccgag
    40381 agttactttg cctgtatctt tatttaaaaa agcagaagtt gttactccag taagagaatc
    40441 agtgtaacta tcaacatatt caagattaga agggaatttt ttcatttttt tcaaatcatt
    40501 aaattgatta atcacgtctc tttttgatag accattatta ttagcattct caacttcgta
    40561 tgacgctttc gaaatagcac ttgtagactc agtattttga ttaattgctt ttaaatctat
    40621 tttatacttc ctactcaaca ttctcacctt cagacggtcc aaaattcaaa taatctctta
    40681 atgttgtctc ttgaccttta tcttttgaaa atttatattc tattttttta tatcctactg
    40741 agcttccttt tatatcacca tctatatata attttagatt tggagagtta ttagttgtaa
    40801 ttggataaat ttctctaagt ttttttacat ttttatcact attgttcatt ttatattgtg
    40861 cttcatagct cggtacgttt tcattagttg ttatatttcc atcttgataa ttttttaaat
    40921 ctttaaaatc agcatactga ctaaaaaatt taaaattttc aattttttgt ttaagttttt
    40981 tgtcttctac attatccaaa agaacaatct tattattttt tagttcaaca cgatattttt
    41041 tctcattatg atttttgcta atatcatcat aaactttatt tacaaaataa taacctgttg
    41101 cctttttggc atttctatta agatataaca ccatgccttc atcatctaat actcctggtt
    41161 tgttactttt tgaaaaacct gtaagtatag tccacgtacc tttatcgcct tttttaaact
    41221 caccatctcg atatccttct ttgtcgtata aatcctcgag attcttaatt ggatacatat
    41281 ctaacgtttt cgcaaaactc tttttgattt gttcttcttt tgaatctgtt ttattcgttt
    41341 gttcctcttt tttctgttca tctttcatat ttccacatcc cccaataaaa atgattaaaa
    41401 gtaatacact catgtacaaa gcaatttttt ttatatactc catcatttca catctctctg
    41461 ttttaatttt ataaacattt gaatatttca tacaacttat taaaatagtg attattcaac
    41521 ttttatgtta tcaaacaaaa ctaacttatt caatttaata catgtaattc attttaactt
    41581 tattattaaa tgttctaacg ttatttttta tattagtaat tatatttatt tatcctcgct
    41641 tggattaaag tagatactat ctgtgaagta aatattttct tccttgtttt caataaaggt
    41701 aaattcaata tttttatatc cgactgatga accttttaaa ttacctgagc ctttcaataa
    41761 taatttgggc gctttttgcg tcgggatatt atatcgcttc cttaattgtt caacattgta
    41821 atcactgttt ttcaattgat attttgcaga gtaaattggt acttctgggt tatatgacac
    41881 ttcgtcctct ttatagtttt ccaattcttt gaaattcccg tattgtacaa agaagttaaa
    41941 ttcttcgatt tcttttttta ctttttcgtc atcgattggt tttaatggaa tcattttatt
    42001 attttccatt ttcacaggat atctttttgt atgattgtgt gtcattccat cgctatcttc
    42061 aacaacttct ctaacaatat aatgcccttt agccgttcta gtatttctgt taatttctaa
    42121 aactgctcct ctagattcca gattttctcc ttttaattgg attttcattt cagatctaat
    42181 tagccaagta cctttatcat cttttttaaa ttcgccatct cgatatcctt ctttgtcgta
    42241 taaatcctcg agattcttaa tagggtagat atctaacgtt ttcgcaaagc tctttttgat
    42301 ttgttcttct tttgaatctc caatgatctt attatcacca tcacaagatg taacaaagat
    42361 gattaaaatc aacaaactaa tgcacaatcc aatccttttt atagacttca ttgttacaca
    42421 ccatcctcat cattcatcat cttcatctac cttcgccggc tggaaattca aagaatctat
    42481 tactgacaaa tcgctttttc caccttttga aaagtcaatt tccaactttt tataacccac
    42541 agaacttcct ttcaaattac catcaatatg catttttaat accggtgctt tatcagtagg
    42601 aatattataa cgacttctta attgcttaac attttcatct ttattgctca ttttaaattt
    42661 tgcgtcataa cttggaacat tctcattaat tgagacatca ccattgttgt agtttttcaa
    42721 ttctttaagg tttgcatatt gtccaaaaaa tttaaagttt tctattctct tttttagatt
    42781 tgtatcttct actttatcta ataagat
//
LOCUS       DALAGD010000004        41769 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.004, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000004 DALAGD010000000
VERSION     DALAGD010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 41769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41769
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.004"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(202..705)
                     /locus_tag="O2701_000163"
     CDS             complement(202..705)
                     /locus_tag="O2701_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCZ4292079.1"
                     /translation="MSKWIIVSDNHTESGVLYQIYEMHPDADVYLHLGDSEFAYDDTE
                     LSLFNRVKGNCDFYPEFENEAVAKYNDVKAFYTHGHLYQVNRTRDLLAEKGLELGCLF
                     AFYGHTHVAKYEYINGVHVINPGSISQSRSSMEETYAEVIIDDQTLHGTINFKNRHHE
                     TISHTTF"
     gene            complement(698..1285)
                     /locus_tag="O2701_000164"
     CDS             complement(698..1285)
                     /locus_tag="O2701_000164"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCZ4292080.1"
                     /translation="MKEIVIASNNQGKINDFKVIFPDYHVIGISELIPDFDVEETGST
                     FEENAILKSEAAAKALNKTVIADDSGLEVFALNGEPGIYSARYAGENKSDEANIEKLL
                     NKLGNTTDRRAQFVCVISMSGPDMETKVFKGTVSGEIADGKYGENGFGYDPIFYVPKL
                     DKTMAQLSKEQKGQISHRRNAINLLQAFLEGDKNV"
     gene            complement(1297..2097)
                     /gene="racE"
                     /locus_tag="O2701_000165"
     CDS             complement(1297..2097)
                     /gene="racE"
                     /locus_tag="O2701_000165"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCZ4292081.1"
                     /translation="MNKPIGVIDSGVGGLTVAKEIMRQLPNETIYYLGDIGRCPYGPR
                     PGEQVKQYTVEIARKLMEFDIKMLVIACNTATAVALEYLQKTLSIPVIGVIEPGARTA
                     IMTTRNQNVLVLGTEGTIKSEAYRTHIKRINPHVEVHGVACPGFVPLVEQMRYSDPTI
                     TSIVIHQTLKRWRNSESDTVILGCTHYPLLYKPIYDYFGGKKTVISSGLETAREVSAL
                     LTFSNEHASYTEHPDHRFFATGDPTHITNIIKEWLNLSVNVERISVND"
     gene            complement(2333..3148)
                     /gene="sdhB"
                     /locus_tag="O2701_000166"
     CDS             complement(2333..3148)
                     /gene="sdhB"
                     /locus_tag="O2701_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HCZ4292082.1"
                     /translation="MTEQSVKNTPQHETQSKPKQKTVKLIIKRQDTSDSKPYEETFEI
                     PYRENLNVIACLMEIRRNPVNIKGEKTTPVVWDMNCLEEVCGACSMVINGRARQSCSA
                     IVDQLEQPIRLEPMNTFPVIRDLQVDRSRMFDNLKRMKAWIPIDGTYDLGPGPRMPEK
                     KRQTAYELSKCMTCGVCLEVCPNVTENNKFVGAQAISQVRLFNLHPTGSMTKDERLNA
                     LMGTGGLQQCGNSQNCVNACPKGIPLTTSIAAMNRETTFHMFKSFFGSDHEVE"
     gene            complement(3148..4914)
                     /gene="sdhA"
                     /locus_tag="O2701_000167"
     CDS             complement(3148..4914)
                     /gene="sdhA"
                     /locus_tag="O2701_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCZ4292083.1"
                     /translation="MAEKHLIVVGGGLAGLMSTIKAAEKGAHVDLFSVVPVKRSHSVC
                     AQGGINGAVNTKGEGDSPWIHFDDTVYGGDFLANQPPVKAMTEAAPKIIHLLDRMGVM
                     FNRTNEGLLDFRRFGGTLHHRTAYAGATTGQQLLYALDEQVRAYEVDGLVTKYEGWEF
                     LGIVKGDDDSARGIVAQNMTTAEIETFGSDAVIMATGGPGIIFGKTTNSMINTGSAAS
                     IVYQQGAIYANGEFIQIHPTAIPGDDKLRLMSESARGEGGRIWTYKDGKPWYFLEEKY
                     PDYGNLVPRDIATREIFDVCINQKLGINGENMVYLDLSHKDPHELDVKLGGIIEIYEK
                     FTGDDPRKVPMKIFPAVHYSMGGLYVDYDQMTNIKGLFAAGECDFSQHGGNRLGANSL
                     LSAIYGGTVAGPNAIDYISNIDRSYTDMDESIFEKRKAEEQERFDKLLAMRGTENAYK
                     LHRELGEIMTANVTVVRENEKLLETDKKIVELMKRYEDIDMEDTQTWSNQAVFFTRQL
                     WNMLVLARVITIGAYNRNESRGAHYKPEFPERNDEEWLKTTMASFQGAFEKPQFTYDD
                     VDVSLIPPRKRDYTSKSKGGKK"
     gene            complement(4966..5580)
                     /locus_tag="O2701_000168"
     CDS             complement(4966..5580)
                     /locus_tag="O2701_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b558 subunit"
                     /protein_id="HCZ4292084.1"
                     /translation="MAQSKNEFYLRRIHSLLGIIPIGAFLVVHLLVNHQATQGAEAFN
                     KASNFMESLPFLIIVEFLFIYIPLLYHGLFGIHIAFTAKENVGHYSIFRNWMFFFQRV
                     SGILTFIFIGIHLWQTRLQKAFYGKEVNYDLMHETLQHPGWAIFYIICIIAVVFHFAN
                     GLWSFLVTWGGLQSPKSQRVFTWVSLIVFLVISYIGVTAIIAFM"
     gene            complement(5904..7685)
                     /gene="uvrC"
                     /locus_tag="O2701_000169"
     CDS             complement(5904..7685)
                     /gene="uvrC"
                     /locus_tag="O2701_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCZ4292085.1"
                     /translation="MEDYKQRIKNKLNVVPMEPGCYLMKDRNDQVIYVGKAKKLRNRL
                     RSYFTGAHDAKTTRLVGEIRRFEFIVTSSETESLLLELNLIKQYQPRYNILLKDDKSY
                     PFIKITKEKYPRLLVTRTVKQGTGKYFGPYPNAYSAQETKKLLDRIYPYRKCDKMPDK
                     LCLYYHIGQCLGPCVYDVDLSKYAQMTKEITDFLNGEDKTILKSLEERMLTASESLDF
                     ERAKEYRDLIQHIQNLTNKQKIMSSDKTIRDVFGYSVDKGWMCIQVFFIRQGNMIKRD
                     TTMIPLQQTEEEEFYTFIGQFYSLNQHILPKEVHVPRNLDKEMIQSVVDTKIVQPARG
                     PKKDMVDLAAHNAKVSLNNKFELISRDESRTIKAIEELGTQMGIQTPIRIEAFDNSNI
                     QGVDPVSAMVTFVDGKPDKKNYRKYKIKTVKGPDDYKSMREVVRRRYSRVLNEGLPLP
                     DLIIVDGGKGHMNGVIDVLQNELGLDIPVAGLQKNDKHQTSELLYGASAEIVPLKKNS
                     QAFYLLHRIQDEVHRFAITFHRQTRQKTGLKSILDDIDGIGNKRKTLLLRSFGSIKKM
                     KEATLEDFKNIGIPENVAKNLHEQLHK"
     gene            7852..7947
                     /locus_tag="O2701_000170"
     CDS             7852..7947
                     /locus_tag="O2701_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292086.1"
                     /translation="MLDYHLTFIDLLIPPFSSRKQYDDLSFNFLY"
     gene            complement(8010..8324)
                     /gene="trxA"
                     /locus_tag="O2701_000171"
     CDS             complement(8010..8324)
                     /gene="trxA"
                     /locus_tag="O2701_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HCZ4292087.1"
                     /translation="MAIVKVTDADFDSKVESGVQLVDFWATWCGPCKMIAPVLEELAA
                     DYEGKADILKLDVDENPSTAAKYEVMSIPTLIVFKDGQPVDKVVGFQPKENLAEVLDK
                     HL"
     gene            complement(8497..10845)
                     /locus_tag="O2701_000172"
     CDS             complement(8497..10845)
                     /locus_tag="O2701_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCZ4292088.1"
                     /translation="MRQKTLDVLEFEKIKSLVANETISDLGLEKVNQMMPATNFETVV
                     FQMEETDEIAQIYNKHRLPSLSGLSKVSAFIHRADIGGVLNVSELNLIKRLIQVQNQF
                     KTFYNQLVEEDEGVKYPILDDKMNQLPVLTDLFQQINETCDTYDLYDNASYELQGIRS
                     KISSTNQRIRQNLDRIVKSQANQKKLSDAIVTVRNERNVIPVKAEYRQDFNGIVHDQS
                     ASGQTLYIEPSSVVEMNNQISRLRHDEAIEKERILTQLTGYVAADKDALLVAEQVMGQ
                     LDFLIAKARYSRSIKGTKPIFKEDRTVYLPKAYHPLLNRETVVANTIEFMEDIETVII
                     TGPNTGGKTVTLKTLGLIIVMAQSGLLIPTLDGSQLSVFKNVYCDIGDEQSIEQSLST
                     FSSHMTNIVEILKHADKHSLVLFDELGAGTDPSEGAALAMSILDHVRKIGSLVMATTH
                     YPELKAYSYNREGVMNASVEFDVDTLSPTYKLLMGVPGRSNAFDISKKLGLSLNIINK
                     AKTMIGTDEKEINEMIESLERNYKRVETQRLELDRLVKEAEQVHDDLSKQYQQFQNYE
                     KSLIEEAKEKANQKIKAATKEADDIIKDLRQLREQKGADVKEHELIDKKKRLDDHYEA
                     KSIKQNVQKQKYDKIVAGDEVKVLSYGQKGEVLEIVNDEEAIVQMGIIKMKLPIEDLE
                     KKQKEKVKPTKMVTRQNRQTIKTELDLRGYRYEDALIELDQYLDQAVLSNYEQVYIIH
                     GKGTGALQKGVQQHLKKHKSVSDFRGGMPSEGGFGVTVATLK"
     gene            complement(10855..12567)
                     /gene="polX"
                     /locus_tag="O2701_000173"
     CDS             complement(10855..12567)
                     /gene="polX"
                     /locus_tag="O2701_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HCZ4292089.1"
                     /translation="MTKKDVIKLLEQIATYMELKGENTFKISAYRKAAQSLELDERPL
                     DEISDVTELKGIGKGVAEVINDYRETGESQYLQQLQEEVPEGLIPLLKIQGLGSKKIA
                     KLYKELNIVDKASLQVACENGKVSELSGFAKKTEQNILEAVKQLGAKKDRYPIDQMRR
                     LNQEIIDYIDTLNYIDQYSSAGSFRRFKEMSKDLDFIISTDNPKAVQQQLLNIPNKVK
                     EVAVGNTKVSLELAYDDETIGVDFRLIEPSAFYHTLQHFTGSKEHNIRIRQLAKARDE
                     KVSEYGIEQADGTLIQYDSEAKIYEHFNVNFIPPAMREDGSEFDKDLSNIITIDDING
                     DIHMHTTYSDGAFSIRDMVEANIAKGYKFMVITDHSQSLRVANGLQVERLLRQNEEIK
                     ALDKEYSEIDIYSGTEMDILPDGSLDYDDEILAQLDYVIGAIHQSFNQSEEQIMERLA
                     NACRNPYVRHIAHPTGRIIGRRDGYKPNIEQLMALAEETNTVLEINANPHRLDLSADI
                     VRKYPNVKLTINTDAHHTNHLDFMNYGVATAQKGFVTKDRVINALSREAFKDFIENNT
                     KLKK"
     gene            complement(12640..13161)
                     /locus_tag="O2701_000174"
     CDS             complement(12640..13161)
                     /locus_tag="O2701_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HCZ4292090.1"
                     /translation="MVIDFIIIIFFVYFVIVGFRRGFWLSMIHLSATIVSLWIASQFY
                     KSIVERLIVFIPYPKTTAFNTTFAFHFNHLQNRFEAIVAFLMITLFCKFILYLIIVTF
                     DKIIAYQNIHIFSRAMGMIVGVFMTIIVLHFTLYLLALYPNEALQHQLKISIVSHSLI
                     FHIPYLSAFTINL"
     gene            complement(13162..13428)
                     /gene="zapA"
                     /locus_tag="O2701_000175"
     CDS             complement(13162..13428)
                     /gene="zapA"
                     /locus_tag="O2701_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCZ4292091.1"
                     /translation="MTQFKNKVNVSINDQLFTIVGEDNPEHIRYVAHLVDDKIKELGY
                     KAAGLDTSRKAILTAVNIMHEKVLLEEENRRLKQQIHKLQQREQ"
     gene            13798..14736
                     /locus_tag="O2701_000176"
     CDS             13798..14736
                     /locus_tag="O2701_000176"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HCZ4292092.1"
                     /translation="MANIVFKLSDKDITTLMSRISFDTENLPQGMKARAKYQNTTVNI
                     YQSGKVMFQGNHAEAVSEELLPQHSQLNTNKTKKKNMANSFLEQTLMYDQFNCIGSDE
                     AGSGDYFGPLTVCAAFVTKEHVPILKTLGVDDSKKLTDTKIVELAEQLVTFIPHSLLT
                     LHNEKYNIQQAKGWTQVKMKAALHNEAIKNVLEKIDSSQLDYIVIDQFAKREVYSHYA
                     LSDIPLPKKTKFETKGESKSLAIAVASIISRYAFVTYMDQISKNINMTIPKGAGAKVD
                     VIAAKIIKKYGLSRLDTISKKHFKNREKAQKILKPL"
     gene            complement(14907..17309)
                     /locus_tag="O2701_000177"
     CDS             complement(14907..17309)
                     /locus_tag="O2701_000177"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCZ4292093.1"
                     /translation="MLISNEWLKEYVTIDDSVSNLAERITRTGIEVDDLIDYTKDIKN
                     LVVGFVKSKEKHPDADKLNVCQVDIGEDEPVQIVCGAPNVDAGQYVIVAKVGGRLPGG
                     IKIKRAKLRGERSEGMICSLQEIGISSNYIPKSFESGIYVFSEAQVPGTDALQALYLD
                     DQVMEFDLTPNRADALSMIGTAYEVAALYNTKMTKPETTSNELDLSANDELTVTIENE
                     DKVPYYSARVVHDVTIEPSPIWMQARLIKAGIRPINNVVDISNYVLLEYGQPLHMFDQ
                     DAIGSQQIVVRQANEGEKMTTLDDTERELLTSDIVITNGQTPIALAGVMGGDFSEVKE
                     QTSNIVIEGAIFDPVSIRHTSRRLNLRSESSSRFEKGIATEFVDEAVDRACYLLQTYA
                     NGKVLKDRVSSGELGAFITPIDITADKINRTIGFDLSQNDIVTIFNQLGFDTEINDDV
                     ITVLVPSRRKDITIKEDLIEEVARIYGYDDIPSTLPVFDKVTSGQLTDRQYKTRMVKE
                     VLEGAGLDQAITYSLVSKEDATAFSMQQRQTIDLLMPMSEAHASLRQSLLPHLIEAAS
                     YNVARKNKDVKLFEIGNVFFANGEGELPDQVEYLSGILTGDYVVNQWQDKKETVDFYL
                     AKGVVDRVSEKLNLEFSYRRADIDGLHPGRTAEILLENKVVGFIGELHPILAADNDLK
                     RTYVFELNFDALMAVSVGYINYQPIPRFPGMSRDIALEVDQNIPAADLLSTIHAHGGN
                     ILKDTLVFDVYQGEHLEKGKKSIAIRLNYLDTEETLTDERVSKVQAEIEAALIEQGAV
                     IR"
     gene            complement(17309..18367)
                     /gene="pheS"
                     /locus_tag="O2701_000178"
     CDS             complement(17309..18367)
                     /gene="pheS"
                     /locus_tag="O2701_000178"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCZ4292094.1"
                     /translation="MSEQQTMSELKQQALVDINEANDERALQEVKVKYLGKKGSVSGL
                     MKLMKDLPNEDKPAFGQKVNELRQTIQNELDERQQMLVKEKLNKQLAEETIDVSLPGR
                     HIEIGSKHPLTRTIEEIEDLFLGLGYEIVNGYEVEQDHYNFEMLNLPKSHPARDMQDS
                     FYITDEILLRTHTSPVQARTMESRHGQGPVKIICPGKVYRRDSDDATHSHQFTQIEGL
                     VVDKNVKMSDLKGTLELLAKKLFGADREIRLRPSYFPFTEPSVEVDVSCFKCKGKGCN
                     VCKHTGWIEILGAGMVHPNVLEMAGFDSSEYSGFAFGMGPDRIAMLKYGIEDIRHFYT
                     NDVRFLDQFKAVEDRGDM"
     gene            complement(18748..19488)
                     /locus_tag="O2701_000179"
     CDS             complement(18748..19488)
                     /locus_tag="O2701_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HCZ4292095.1"
                     /translation="MEQITSAQNNRIKQANKLKKKRERDKTGLALIEGVHLIEEAYQS
                     GIVITQLFAIEPARLDQQIIAYAQEVFEINMKVAESLSGTVTPQGFFAIIEKPHYDIS
                     KAQQVLLIDRVQDPGNLGTLIRTADAAGMDAVIMEKGTTDPYQDKVLRASQGSVFHLP
                     VMTQDLDTFITQFNGPVYGTALENAVAYKEVTSSDSFALLLGNEGEGVNPELLAHTTQ
                     NLIIPIYGKAESLNVAIAGSILLYHLKG"
     gene            complement(19728..19835)
                     /locus_tag="O2701_000180"
     CDS             complement(19728..19835)
                     /locus_tag="O2701_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292096.1"
                     /translation="MVLLAFVTTIHDTDDGRFFNEHKHANNKLLKHSLI"
     gene            complement(19872..20195)
                     /gene="isdG"
                     /locus_tag="O2701_000181"
     CDS             complement(19872..20195)
                     /gene="isdG"
                     /locus_tag="O2701_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdG"
                     /protein_id="HCZ4292097.1"
                     /translation="MKFMAENRLTLTKGTAKDIIERFYTRHGIETLEGFDGMFVTQTL
                     EQEDFDEVKILTVWKSKQAFTDWLKSDVFKAAHKHVRSKNEDESSPIINNKVITYDIG
                     YSYMK"
     gene            complement(20214..20948)
                     /gene="srtB"
                     /locus_tag="O2701_000182"
     CDS             complement(20214..20948)
                     /gene="srtB"
                     /locus_tag="O2701_000182"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HCZ4292098.1"
                     /translation="MRMKRFLTIVQILLVVIIIIFGYKIVQTYIEDKQERANYEKLQQ
                     KFQMLMSKHQEHVRPQFESLEKINKDIVGWIKLSGTSLNYPVLQGKTNHDYLNLDFER
                     EHRRKGSIFMDFRNELKNLNHNTILYGHHVGDNTMFDVLEDYLKQSFYEKHKIIEFDN
                     KYGKYQLQVFSAYKTTTKDNYIRTDFENDQDYQQFLDETKRKSVINSDVNVTVKDRIM
                     TLSTCEDAYSETTKRIVVVAKIIKVS"
     gene            complement(21010..21975)
                     /gene="isdF"
                     /locus_tag="O2701_000183"
     CDS             complement(21010..21975)
                     /gene="isdF"
                     /locus_tag="O2701_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin ABC transporter permease protein IsdF"
                     /protein_id="HCZ4292099.1"
                     /translation="MIKNKKKLLFLCLLVILIATAYISFVTGTIKLSFNDLFTKFTTG
                     SNEAVDSIIDLRLPRILIALMVGAMLAVSGALLQAALQNPLAEANIIGVSSGALIMRA
                     LCMLFIPQLYFYLPLLSFIGGLIPFLIIILLHSKFRFNAVSMILVGVALFVLLNGVLE
                     ILTQNPLMKIPQGLTMKIWSDVYILAVSALLGLILTLLLSPKLNLLNLDDIQARSIGF
                     NIDRYRWLTGLLAVFLASATVAIVGQLAFLGIIVPHVVRKLVGGNYRVLIPFSTVIGA
                     WLLLVADLLGRVIQPPLEIPANAILMIVGGPMLIYLICQSQRNRI"
     gene            complement(21988..22866)
                     /gene="isdE"
                     /locus_tag="O2701_000184"
     CDS             complement(21988..22866)
                     /gene="isdE"
                     /locus_tag="O2701_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HCZ4292100.1"
                     /translation="MRIIKYLTILVISVVILTSCQSSSSQESTKSGEFRIVPTTVALT
                     MTLDKLDLPIVGKPTSYKTLPNRYKDVPEIGQPMEPNVEAVKKLKPTHVLSVSTIKDE
                     MQPFYKQLNMKGYFYDFDSLKGMQKSITQLGDQFNRKAQAKELNDHLNSVKQKIENKA
                     AKQKKHPKVLILMGVPGSYLVATDKSYIGDLVKIAGGENVIKVKDRQYISSNTENLLN
                     INPDIILRLPHGMPEEVKKMFQKEFKQNDIWKHFKAVKNNHVYDLEEVPFGITANVDA
                     DKAMTQLYDLFYKDKK"
     gene            complement(22863..23939)
                     /gene="isdD"
                     /locus_tag="O2701_000185"
     CDS             complement(22863..23939)
                     /gene="isdD"
                     /locus_tag="O2701_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated surface determinant protein IsdD"
                     /protein_id="HCZ4292101.1"
                     /translation="MRNVKQIATKSIIAIISLGILTYTTMIGSVLADEIKYPSAKFNQ
                     PEAKDKTELTTSIFDEKIKENKALELLIFNQENKNVTEEQQLVDEKAQLISDMTGKIY
                     LQVKLKGQIDKEQLVFQNDKNEEFPFVIKDEKDDTIVRILIEQHMDKINMHVKTLAEK
                     KNLDNKEMVYSIHFKEKKVQHDDAKEVPSKHQNQENNQDQLKKDIDDKKDSQKSDTKE
                     RRTSLFTEKGLNDIPVQKDKVQQDSNKKIENERPKASGTLKVENSPPTIKKVENNHKE
                     QPKHKDEKSKKEKKKVVEKEKALPAFNRDDDSKNSSQLSSDIKELDEPNHKKQYMLFA
                     AGIVLATILLISAHLYSRKRGNQV"
     gene            complement(23939..24622)
                     /gene="isdC"
                     /locus_tag="O2701_000186"
     CDS             complement(23939..24622)
                     /gene="isdC"
                     /locus_tag="O2701_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdC"
                     /protein_id="HCZ4292102.1"
                     /translation="MKNILKVFNTTILALIIIIATFSNSANAADSGTLNYEVYKYNTN
                     DTSIANDYFNKPAKYIKKNGKLYVQITVNHSHWITGMSIEGHKENIISKNTAKDERTS
                     EFEVSKLNGKIDGKIDVYIDEKVNGKPFKYDHHYNITYKFNGPTDVAGANAPGKDDKN
                     SASGSDKGSDGTTTGQSESNSSNKDKVENPQTNAGTPAYIYAIPVASLALLIAITLFV
                     RKKSKGNVE"
     gene            24831..25883
                     /gene="isdA"
                     /locus_tag="O2701_000187"
     CDS             24831..25883
                     /gene="isdA"
                     /locus_tag="O2701_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored heme-scavenging protein IsdA"
                     /protein_id="HCZ4292103.1"
                     /translation="MTKHYLNSKYQSEQRSSAMKKITMGTASIILGSLVYIGADSQQV
                     NAATEATNATNNQSTQVSQATSQPINFQVQKDGSSEKSHMDDYMQHPGKVIKQNNKYY
                     FQTVLNNASFWKEYKFYNANNQELATTVVNDNKKADTRTINVAVEPGYKSLTTKVHIV
                     VPQINYNHRYTTHLEFEKAIPTLADAAKPNNVKPVQPKPAQPKTPTEQTKPVQPKVEK
                     VKPTVTTTSKVEDNHSTKVVSTDTTKDQTKTQTAHTVKTAQTAQEQNKVQTPVKDVAT
                     AKSESNNQAVSDNKSQQTNKVTKHNETPKQASKAKELPKTGLTSVDNFISTVAFATLA
                     LLGSLSLLLFKRKESK"
     gene            26086..28023
                     /gene="isdB"
                     /locus_tag="O2701_000188"
     CDS             26086..28023
                     /gene="isdB"
                     /locus_tag="O2701_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdB"
                     /protein_id="HCZ4292104.1"
                     /translation="MNKQQKEFKSFYSIRKSSLGVASVAISTLLLLMSNGEAQAAAEE
                     TGGTNTEAQPKTEAVASPTTTSEKAPETKPVANAVSVSNKEVEAPTSETKEAKEVKEV
                     KAPKETKEVKPAAKATNNTYPILNQELREAIKNPAIKDKDHSAPNSRPIDFEMKKKDG
                     TQQFYHYASSVKPARVIFTDSKPEIELGLQSGQFWRKFEVYEGDKKLPIKLVSYDTVK
                     DYAYIRFSVSNGTKAVKIVSSTHFNNKEEKYDYTLMEFAQPIYNSADKFKTEEDYKAE
                     KLLAPYKKAKTLERQVYELNKIQDKLPEKLKAEYKKKLEDTKKALDEQVKSAITEFQN
                     VQPTNEKMTDLQDTKYVVYESVENNESMMDTFVKHPIKTGMLNGKKYMVMETTNDDYW
                     KDFMVEGQRVRTISKDAKNNTRTIIFPYVEGKTLYDAIVKVHVKTIDYDGQYHVRIVD
                     KEAFTKANTDKSNKKEQQDNSAKKEATPATPSKPTPSPVEKESQKQDSQKDDNKQLPS
                     VEKENDASSESGKDKTPATKPTKGEVESSSTTPTKVVSTTQNVAKPTTASSKTTKDVV
                     QTSAGSSEAKDSAPLQKANIKNTNDGHTQSQNNKNTQENKAKSLPQTGEESNKDMTLP
                     LMALLALSSIVAFVLPRKRKN"
     gene            complement(28177..28350)
                     /gene="rpmF"
                     /locus_tag="O2701_000189"
     CDS             complement(28177..28350)
                     /gene="rpmF"
                     /locus_tag="O2701_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCZ4292105.1"
                     /translation="MAVPKRRTSKTRKNKRRTHFKISVPGMTECPNCGEYKLSHRVCK
                     NCGSYNGEEVAAK"
     gene            complement(28430..28987)
                     /locus_tag="O2701_000190"
     CDS             complement(28430..28987)
                     /locus_tag="O2701_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HCZ4292106.1"
                     /translation="MKWSITQLRKYQGKPFEFDQTVSFDNLKESLDLIDLSPITIQGQ
                     LTIKSTEVVADIHITGTYTMPCARTLVPVKVPLDVTTTEVFDLEGYNQYNDDQDDVDE
                     HYHIIKDGMVNLQDIVEDIVIIEKPMRAYSEQSDQMLTVGNGWEVIDEDQLDELAKQQ
                     EQDDSESRQVDPRLQKLQQLYDKEQ"
     gene            29114..30253
                     /locus_tag="O2701_000191"
     CDS             29114..30253
                     /locus_tag="O2701_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HCZ4292107.1"
                     /translation="MKSVGLITEYNPFHNGHQYHINQSKKLTNADVTIAIMSGNFVMR
                     GEPAIYNKFTRAKMALSTADLVIELPATASLSSGDHFAELAVKVADYMSVDTIAFGSE
                     NNDIKTLKQLAHSINEIEQSESFSQKVKEGKSYPRIISELLEHHEALASPNNILGISY
                     LKAIAKNAKNINAISIKRENAQHHDSLIQHHQFASGTSIRTSIISQDDHWHHVVPKDI
                     QHLYVTPHITLNQIFPYLKYQIIAMTTDSLKNIYTVTEGFENRLKSNIYEATDFHHFV
                     KLLKTKRYTYTHIQRLLMNVLLNIKPTDVTSNIHAVKVLAMNDRGRQYLKHLKTAFPE
                     RQYITNINKSNAHYFTNEIKATHIYNAISGQQQTDFNTPVIQQYR"
     gene            complement(30315..30797)
                     /gene="coaD"
                     /locus_tag="O2701_000192"
     CDS             complement(30315..30797)
                     /gene="coaD"
                     /locus_tag="O2701_000192"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCZ4292108.1"
                     /translation="MEHTIAVIPGSFDPITYGHLDIIERSTDRFDEIHVCVLKNSKKE
                     GTFSLEERMDLIEQSVKHLPNVKVHQFSGLLVDYCEQVGAKTIIRGLRAVSDFEYELR
                     LTSMNKKLNNEIETLYMMSSTNYSFISSSIVKEVAAYRADISEFVPPYVEKALKKKFK
                     "
     gene            complement(30799..31341)
                     /gene="rsmD"
                     /locus_tag="O2701_000193"
     CDS             complement(30799..31341)
                     /gene="rsmD"
                     /locus_tag="O2701_000193"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HCZ4292109.1"
                     /translation="MRVIAGKHKSKALESMEGRNTRPTMDKVKEGIFNSLYDVSGIGL
                     DLFAGSGALGIEALSRGMDKVIFVDQNFKAVKVIKSNLANLDLEAQSEVYKNNADRAL
                     KALSKRDIQFDVIFLDPPYNKGLIDKALKLISEFNLLKENGIIVCEFSNHEEIDYQPF
                     NMIKRYHYGLTDTLLLEKGE"
     gene            31411..31800
                     /locus_tag="O2701_000194"
     CDS             31411..31800
                     /locus_tag="O2701_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292110.1"
                     /translation="MKQTFITLGEGLTDLFEFMTMIEYNHQRIDKIIYFHSPQAENKK
                     SSVAIIMNPTTGNHFQAFYIMINAIKYPYPDSNKKFQMINDCAEKFDIPILGIDVQPP
                     QAFHDLSLYYNYLISVLRLQKWIPELQ"
     gene            complement(31803..32057)
                     /locus_tag="O2701_000195"
     CDS             complement(31803..32057)
                     /locus_tag="O2701_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HCZ4292111.1"
                     /translation="MNLIPRTSIVVYLKHMKHERQIRKYGHIVHSNRDRKFVIMYVNE
                     QDVDQIVHKLMQLKYVRHIDGSPYKYLKKTYEKEKHEIYN"
     gene            32296..33222
                     /locus_tag="O2701_000196"
     CDS             32296..33222
                     /locus_tag="O2701_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCZ4292112.1"
                     /translation="MKKVNRNILNAVLVGAGFLSSLLMINKNKVITKKQTIPAFFKGN
                     APYIFAHRGGMALRPEQTQLAFDYAKQLGVDGFETDVRLTKDQQLIVFHDATVDRTTN
                     GSGKVSAHTLAELKKLDAAYHFKDINGLTPYRGHAHTAILTFDELLKQYPDMYINVDL
                     KDAPESYEGSIAPQIMFDTIAENQAFDRVLVTSFYKEQIVRFNKIAQGSVAIGASQQE
                     VTEAFLKYHLLGGRYYQPLAQTFQMPTHFKGIDLTSSRFIKWLNDMNIIPGYYGVNSI
                     NLMNDLYQKGAHTIVTDRPDLAQQFKQTIPNK"
     gene            complement(33285..33719)
                     /locus_tag="O2701_000197"
     CDS             complement(33285..33719)
                     /locus_tag="O2701_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCZ4292113.1"
                     /translation="MINEASLAILDDIDELADMIVASDIYASFEQAKQALENNDEAHL
                     LYQSFLKSKEKYDEVMRFGKYHPDYKKVMLETRQRKRAYEMLDVVMHYKAREMALQHL
                     IDEVVTKIAYAVSEHVKIETGNPFFQTSHSGCATGGSCNCSL"
     gene            complement(33735..34772)
                     /locus_tag="O2701_000198"
     CDS             complement(33735..34772)
                     /locus_tag="O2701_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HCZ4292114.1"
                     /translation="MKKLIIRVVGVLFLVGFLIYLFYSPRLKFDVLENPNKGNKVNRS
                     EQVNKSNNHAENPKPKEGVGTWVGKDIKVLTSKFGQADRVYPFRDGYKNYVFKDKNSY
                     YIVSTKREEIVSVYATGEKVNVSPLKIGQHSAEIFNHTSINPEPSFKVDGKKYEFELS
                     DEDLKTQTLIKYGDIYAQVYSDQQSKKVLSVRFLTKEMLADIEPYQLNSNSTSEEHNK
                     RPVEQNPNQLISLYEVTNEMRKLKGLKPLKINSDLAHIASNNLYEATSNGSDSVEFTE
                     DALRGQLDKNHVTYKTTAQNVGYAFNDVPTLIHSWMNSDIHRSRLLNSKYDEMGGDVM
                     RDYYSLIFLEK"
     gene            complement(35099..35560)
                     /locus_tag="O2701_000199"
     CDS             complement(35099..35560)
                     /locus_tag="O2701_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HCZ4292115.1"
                     /translation="MGVPILPTISTTCIVISAILIAIGWRLIWKREINKHKNVMLAAA
                     VFALTFFLIYASRTIFIGNTAFGGPASIKKYYTIFLFFHINLATIGGILGLVQIITAF
                     KDKYNVHRKFGPFASVIWFCTAITGVAVYLLLYVLYPGGETTSLIKATFGH"
     gene            complement(35585..36496)
                     /locus_tag="O2701_000200"
     CDS             complement(35585..36496)
                     /locus_tag="O2701_000200"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCZ4292116.1"
                     /translation="MSKEHTLSQNISRVNFKELQQIIKMGLVQGNLIPAFAGAWLAVV
                     MTNHSFLSSIPQILLMLFGSTLIMGGACALNNYYDQDIDRIMPSKQNRPTVNNRITDQ
                     NLLLLSFGMMLVGEICLFLLNIPSGVLGLMGIVGYVSYYSIWSKRHTTWNTVIGSFPG
                     AVPPLIGWVAIEGQISLTAIALFLVVFCWQPIHFYALAIKRKDEYALANIPMLPSVKG
                     FKRTRVSMFIWLIILLPVPLLLINLGVVFVVLATLLNLGWIALGLTTFKKNSDQTKWA
                     TQMFIYSLNYLVIFFVLAVIVSLLTLI"
     gene            36936..37847
                     /locus_tag="O2701_000201"
     CDS             36936..37847
                     /locus_tag="O2701_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HCZ4292117.1"
                     /translation="MFGKKNLKWLGVVATLMMTFVQLGGALVTKTGSADGCGSSWPLC
                     HGALIPEFFPIDTIIELSHRAVSALSLLMVLWLVITAWKHIGYIKEIKPLSIISVGFL
                     LLQALIGAAAVIWQQNDYVLALHFGISLISFSSVFLITLIIFSIDQKYEADELYIKKP
                     LRRLTWLMAIIIYCGVYTGALVRHADASLAYGGWPLPFHDLVPHSEQDWVQLTHRIMA
                     FIVFTIIMITYIHAVKNYPNNRTVHYGYTAAFILVILQVITGALSIMTNVNLIIALFH
                     ALFITYLFGMTTYFIMLMLRSVRSDKQ"
     gene            complement(37987..41439)
                     /locus_tag="O2701_000202"
     CDS             complement(37987..41439)
                     /locus_tag="O2701_000202"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HCZ4292118.1"
                     /translation="MKQIKKLLVANRGEIAIRIFRAAAELDISTVAIYSNEDKSSLHR
                     YKADESYLVGSDLGPAESYLNIERIIDVAKQANVDAIHPGYGFLSENEQFARRCAEEG
                     IKFIGPHLEHLDMFGDKVKARTTAIKADLPVIPGTDGPIKSYELAKEFAEEAGFPLMI
                     KATSGGGGKGMRIVREESELEDAFHRAKSEAEKSFGNSEVYIERYIDNPKHIEVQVIG
                     DEHGNIVHLFERDCSVQRRHQKVVEVAPSVGLSSTLRQRICDAAIQLMENIKYVNAGT
                     VEFLVSGDEFFFIEVNPRVQVEHTITEMVTGIDIVKTQILVAAGADLFGEEINMPQQK
                     DITTLGYAIQCRITTEDPLNDFMPDTGTIIAYRSSGGFGVRLDAGDGFQGAEISPYYD
                     SLLVKLSTHAISFKQAEEKMVRSLREMRIRGVKTNIPFLINVMKNKKFTSGDYTTKFI
                     EETPELFNIQPSLDRGTKTLEYIGNVTINGFPNVEKRPKPDYELASIPTVSSSKIASF
                     SGTKQLLDEVGPKGVAEWVKKQDDVLLTDTTFRDAHQSLLATRVRTKDMINIASKTAD
                     VFKDGFSLEMWGGATFDVAYNFLKENPWERLERLRKAIPNVLFQMLLRASNAVGYKNY
                     PDNVIHKFVQESAKAGIDVFRIFDSLNWVDQMKVANEAVQEAGKISEGTICYTGDILN
                     PERSNIYTLEYYVKLAKELEREGFHILAIKDMAGLLKPKAAYELIGELKSAVDLPIHL
                     HTHDTSGNGLLTYKQAIDAGVDIIDTAVASMSGLTSQPSANSLYYALNGFPRHLRTDI
                     EGMESLSHYWSTVRTYYSDFESDIKSPNTEIYQHEMPGGQYSNLSQQAKSLGLGERFD
                     EVKDMYRRVNFLFGDIVKVTPSSKVVGDMALYMVQNDLDEQSVITDGYKLDFPESVVS
                     FFKGEIGQPVNGFNKDLQAVILKGQEALTARPGEYLEPVDFEKVRELLEEEQQGPVTE
                     QDIISYVLYPKVYEQYIQTRNQYGNLSLLDTPTFFFGMRNGETVEIEIDKGKRLIIKL
                     ETISEPDENGNRTIYYAMNGQARRIYIKDENVHTNANVKPKADKSNPSHIGAQMPGSV
                     TEVKVSVGETVKANQPLLITEAMKMETTIQAPFDGVIKQVTVNNGDTIATGDLLIEIE
                     KATD"
BASE COUNT        13562 a         7744 c         5827 g        14636 t
ORIGIN      
        1 accaaataat caacatgatt caaccatata gggttatcaa caatcacatt ctccaaaatt
       61 acaattcttt tcataacata aatgtgttat tcattccaat tatcttataa aattgtcaga
      121 aatactttta aacaaacatt catgacacga cctcctagtt aaatcagtct ataaactaaa
      181 aaaagccata gctacttcta tttaaaaagt agtatgactg attgtttcgt gatgtcgatt
      241 tttgaaattg atggtgccat gtaaagtttg atcatcaata ataacttcag catatgtttc
      301 ttccattgaa cttctagatt gagatatact tccaggatta ataacatgaa caccattaat
      361 atactcatat tttgccacat gtgtatgtcc ataaaatgca aacaaacaac ctaattcaag
      421 tcctttttca gctaataaat ctcttgttcg attgacttga tataaatgtc catgagtata
      481 aaatgctttc acgtcattat attttgcgac cgcttcattt tcaaattctg ggtaaaaatc
      541 acaattgcct tttactctat taaataagct aagttccgta tcatcatacg cgaattctga
      601 atctcctaaa tgtaaatata catctgcatc tgggtgcatt tcataaattt gatataaaac
      661 gcctgattca gtatggttat cactcacaat aatccattta gacattttta tcaccttcaa
      721 gaaaagcttg aagtaaatta atcgcatttc gtctatgact aatttgccct ttttgttctt
      781 ttgaaagttg agccatggtt ttatctaatt tcggtacata aaatatcgga tcatatccga
      841 aaccattttc gccatatttt ccatctgcaa tttcacctga aacagtacct ttaaatactt
      901 ttgtttccat atcagggcca ctcatactta tgacacaaac aaattgcgca cgacgatcag
      961 ttgtattacc aagcttattt aataattttt caatatttgc ttcatcgctt ttattttcac
     1021 cagcataacg tgcagagtat atacctggct caccatttaa tgcaaaaact tctagtccac
     1081 tgtcatcagc tatgaccgtt ttattcaatg cttttgcagc agcttctgat tttaatatag
     1141 cattttcttc aaatgttgat cctgtttctt ccacatcaaa atctggtatt agttctgaaa
     1201 tacctattac gtggtaatct ggaaatatta ctttaaagtc atttattttc ccttgattat
     1261 tcgatgcaat aacaatctct ttcattaaaa atcctcctag tcattcactg atatacgttc
     1321 cacattgaca gataaattta accactcttt gataatgtta gtaatgtgag taggatcacc
     1381 tgttgcaaaa aatcgatgat ctggatgttc agtataactt gcatgttcat tactaaatgt
     1441 tagcaatgca ctaacttcac gagccgtttc taatccagac gaaatcactg tctttttacc
     1501 accaaaataa tcatagatag gtttatagag caatggatag tgggtacatc ctaaaatgac
     1561 agtatcagac tcactattac gccaacgttt cagtgtttga tgaatgacaa tgcttgtaat
     1621 tgttggatca ctatatctca tttgttctac aagtggcaca aaacctggac aggcaacgcc
     1681 atgtacctct acatgtggat tgatacgttt aatatgcgta cgatatgctt cagatttaat
     1741 tgtgccttcc gttcctagta ctaatacatt ttgatttcta gtagtcatta ttgctgttct
     1801 agcacctggt tcaattacgc caatcactgg gattgataag gtcttttgta aatattctaa
     1861 agctacagca gttgctgtat tacaagcaat cacgagcatt tttatatcaa attccattaa
     1921 tttacgagcg atttcaactg tatattgttt tacttgttct cctggtcttg gcccatatgg
     1981 acatcgtcca atatcaccta agtaataaat cgtctcattt ggcaactgac gcataatttc
     2041 tttagctact gtcaaacctc cgacaccaga gtctattaca cctattggtt tattcatatt
     2101 cggtcatcct tacttttaat ttaattttta gtttatcata actaagcatt ggattttagt
     2161 attatgcact gtgtttacca tttttgtcat tataatattt attttaaatc agcccactat
     2221 catattgtca tgtaatctgc ttattaaaaa aatcccttcc aagttattgt gtatctccat
     2281 tcaatttaat tttgaaagga acataacatt ttaactcaaa agggattaat ttttattcta
     2341 cttcatggtc tgaaccaaag aatgatttaa acatgtggaa tgttgtttct ctgttcattg
     2401 ctgcaatgga tgttgttaat ggaatacctt tagggcaagc attaacacag ttttgtgaat
     2461 taccacactg ctgtaagcca ccagtaccca ttaatgcatt taaacgttca tctttagtca
     2521 tagatcctgt tgggtgcaaa ttaaacaaac gaacttgcga gattgcttgt gcaccaacga
     2581 atttattatt ttcagtaaca ttaggacaaa cctctaaaca tacaccacat gtcatacatt
     2641 tagataattc ataagctgtt tgacgttttt tctctggcat acgtggtccc ggacctaaat
     2701 catacgttcc atcaattggg atccatgctt tcatacgttt taagttatcg aacattctag
     2761 aacgatcaac ttgtaagtca cggataactg ggaaagtatt cattggctct aaacgaatag
     2821 gttgttctaa ttgatcaaca atcgcagaac aagattgtct tgcacgacca ttgataacca
     2881 tagaacatgc tccacatact tcttctaagc agttcatatc ccagacaaca ggtgttgttt
     2941 tttcaccttt aatattaact gggttacgtc taatttccat taaacaagca atgacgttta
     3001 aattttcacg atatggaatt tcaaatgttt cttcataagg cttagaatca cttgtatctt
     3061 gtcgtttaat aattaatttt actgtttttt gtttcggttt agattgtgtt tcatgttgtg
     3121 gagtgttttt cactgattgt tcagtcatta ttttttaccc cctttagact tacttgtgta
     3181 atcacgttta cgaggtggta ttaaactcac atcgacgtca tcataagtaa actgcggttt
     3241 ttcaaatgcg ccttggaatg aggccattgt cgtttttaac cactcttcat cattacgctc
     3301 tgggaattct ggtttataat gggcaccgcg tgattcgtta cggttatatg caccaatcgt
     3361 aataacacgt gcaagtacta acatgttcca tagttgacgg gtaaagaata ccgcttggtt
     3421 actccaagtt tgagtatctt ccatatcaat atcttcataa cgtttcatca attcaacaat
     3481 ctttttatct gtttctaaca gtttttcatt ttcacgaaca acagttacat ttgctgtcat
     3541 aatttcacca agttcacggt gtaatttata tgcattttct gtaccgcgca tagctaataa
     3601 tttatcaaaa cgttcttgct cttcagcttt acgcttttca aaaatacttt cgtccatatc
     3661 agtatatgat cgatcaatat ttgaaatata atcaatcgcg tttggacctg ctactgtacc
     3721 accataaatc gctgataaca atgaattggc acctaagcgg ttaccaccat gttgagagaa
     3781 gtcacattct ccagctgcaa ataacccttt aatatttgtc atttgatcat aatctacata
     3841 tagaccaccc attgaatagt gaacagctgg gaaaatcttc attggtactt tgcgtgggtc
     3901 atcaccagtg aatttttcat aaatctcaat gataccacct agttttacat ctaactcatg
     3961 tggatcttta tgtgacaaat caagatatac catgttttcg ccatttatac ctaatttttg
     4021 gttaatacat acatcgaaaa tttcacgcgt tgcgatatca cgaggtacta agttaccata
     4081 atcaggatat ttctcttcta agaagtacca aggcttacca tctttatatg tccaaattcg
     4141 tccaccttca ccacgtgctg attcactcat tagtcgcagt ttatcatcac cagggattgc
     4201 agtaggatga atttgaatga actcaccatt agcataaata gcgccttgtt ggtaaacaat
     4261 ggaagccgct gatcctgtat taatcattga gtttgttgtt ttaccgaaaa taataccagg
     4321 gccacccgtt gccataataa ctgcatctga accaaatgtt tcaatctcag cagttgtcat
     4381 attttgtgca acgatacctc ttgcactatc atcgtcacct ttaactatgc caaggaattc
     4441 ccatccttca tacttcgtaa ctaatccatc tacttcatat gcacgaactt gttcatccaa
     4501 tgcatataat aattgttgtc cagttgttgc ccctgcatat gctgttctgt gatgtaatgt
     4561 accaccgaaa cgtctaaaat ctaatagacc ttcatttgtt ctattgaaca ttacgcccat
     4621 acggtctaat aaatgaataa ttttaggtgc tgcctctgtc atcgctttaa caggtggttg
     4681 gtttgcaagg aaatcgccac catacactgt atcatcaaag tgaatccaag gagaatcgcc
     4741 ttccccttta gtattgaccg caccattaat gccaccttgg gcacaaacag agtgcgaacg
     4801 ctttactggt acaactgaga acaaatctac atgtgcacct ttttctgccg ctttaattgt
     4861 tgacattaag cccgctaggc cacctccgac aacaataaga tgtttctctg ccataaaaat
     4921 ttcactcccc taaattttca atctatattt gttaaatgcg atgtattaca taaaggcaat
     4981 aattgcagta acaccaatat acgaaataac taaaaatacg attaatgaaa cccatgtaaa
     5041 tactcgttgt gattttggag attgaagtcc accccaagta actaagaatg accataagcc
     5101 atttgcaaag tggaacacaa cagcaataat acaaataata taaaatattg cccatccagg
     5161 atgttgcaat gtttcgtgca ttaaatcgta attcacttct ttgccgtaaa atgctttttg
     5221 taaacgtgtt tgccataaat ggataccaat aaagataaat gttaagatac cactcactct
     5281 ttggaagaag aacatccagt ttctaaaaat cgagtaatgt ccaacatttt cttttgctgt
     5341 aaatgcaatg tgtataccaa acaaaccgtg atataacaac ggaatgtata taaataaaaa
     5401 ttctacaata attagaaatg gtaatgattc cataaagtta gatgccttat taaacgcttc
     5461 agcaccttgt gttgcttggt gattcactaa taaatgaacg accaaaaatg cacctattgg
     5521 gataatacct aataacgagt gaatacgtct tagataaaat tcattttttg attgagccaa
     5581 aaggagtccc ccctgtgaac gaatatttaa tttattgagc tatttatatt aaacgtacgc
     5641 ttaaccccct aaagtgatat cgtttctagc gttgtcatta tcattaagcg aaacatttta
     5701 aagacaaata cacactgcac gatcaccaaa ctgcatgtcg aacaatgtaa catttggatt
     5761 cgatatttaa aattgcttgt gatgataaac tttctcattt agaaaacgct tccacgtaca
     5821 ttcaaaaaaa taactttgtt aaccatattg taacattatt tcatatattt tggggcatga
     5881 gaatgattct cacgcccagt aatttattta tgcaattgtt catgtaggtt ctttgcgacg
     5941 ttttcaggaa tacctatatt tttaaaatct tcaagtgtag cttccttcat tttcttgatt
     6001 gaaccgaatg aacgcaataa taatgtttta cgtttgttac cgataccatc tatatcatca
     6061 agtattgatt tcaagcctgt cttttgacgt gtttgtctat gaaatgtgat tgcgaatctg
     6121 tgaacctcat cttggatacg gtgcaacaaa taaaatgcct ggctattttt cttcagtggt
     6181 acaatttctg cactagcgcc atataataat tcagatgttt ggtgtttatc atttttctgc
     6241 aaacctgcaa cagggatatc aagacctaat tcgttttgta gcacatcaat aaccccgttc
     6301 atatgtcctt taccaccatc tactattatt aaatcaggta atggtaatcc ttcgtttaaa
     6361 acgcgagaat atcgtcgtct tactacttct ctcattgatt tgtaatcatc tggaccttta
     6421 accgttttga ttttatactt tctataattt ttcttatctg gtttaccgtc gacaaatgta
     6481 accattgctg acactggatc cacaccttga atattagaat tatcgaatgc ttcaattcta
     6541 attggtgttt gaattcccat ttgtgttcca agttcttcaa tagctttaat cgttctggac
     6601 tcatcacgtg atattaattc aaatttatta tttaaggata ctttagcgtt atgtgcagct
     6661 aggtcaacca tatctttttt gggacctcgc gcgggttgaa cgattttagt gtccacaaca
     6721 gattgaatca tttctttatc caaattacgt ggtacatgaa cttccttagg taaaatatgt
     6781 tggtttaagc tataaaattg tccaataaat gtataaaatt cttcttcttc tgtttgctgt
     6841 aatggaatca tcgttgtatc tcgctttatc atattacctt gtcgtataaa gaaaacttgg
     6901 atacacatcc atcctttatc aacactataa ccaaagacat cacgaatcgt tttatctgat
     6961 gacataattt tttgtttgtt tgtcagattt tgaatatgtt gaattaaatc tctatattct
     7021 ttagcccgtt caaaatcaag tgattcactt gcagttaaca ttcgctcttc taaacttttt
     7081 aaaattgttt tgtcttcccc attcagaaaa tcagtaattt ccttcgtcat ttgtgcgtat
     7141 ttactcaaat caacgtcata tacacatggt cctaaacatt gtccaatatg gtaataaaga
     7201 cataatttat ctggcatctt atcacatttg cgatatggat atattctgtc taataacttt
     7261 ttagtttctt gagcagaata tgcattcgga tacggtccga aatatttgcc agtaccttgt
     7321 tttacagttc tcgtcactag tagtctagga tatttctcct tcgtaatttt aataaatgga
     7381 taacttttat catcctttaa taatatatta tatcttggtt gatattgttt aatcagattc
     7441 aattccagta aaagtgattc tgtttcactt gacgtgacaa taaactcaaa gcgacgtatt
     7501 tcaccaacca gtctcgttgt tttagcatca tgagcacccg taaaatatga tcgcaatcga
     7561 tttcttagct ttttagcttt gccaacatat atcacttgat cattacgatc tttcattaaa
     7621 tagcatcctg gttccatagg tacgacattt aatttatttt taattcgttg cttatagtct
     7681 tccaaaaata tccccccctt ctaattttaa aatgaatgat gctacaaaag tgcttcaaat
     7741 ttatcaccac taattctagt taataatcaa catcttcgag tatgactttt catgcacatc
     7801 atgtaatatt gaacatggtt ttatcaaacc aactgctaca aatctatcag catgctagat
     7861 tatcatttaa cgttcataga tttattaatc ccaccattct caagtcgtaa acaatatgat
     7921 gacttatcat tcaacttttt atattaaaaa cacaaacaaa aactggagca attcattaaa
     7981 gaaatgcccc agtcgtcatt ggttgtaact tataaatgtt tatctaaaac ttcagctaag
     8041 ttttcttttg gttggaaacc aacaacttta tcaactggtt gaccgtcttt aaagacgatt
     8101 aatgttggaa tactcatcac ttcatattta gctgcagttg atggattttc atcaacatct
     8161 aattttaaaa tgtcagcttt accttcatag tcagctgcta attcttctaa taccggagcg
     8221 atcattttac atggaccaca ccatgttgcc caaaaatcta ctagttgtac accagattct
     8281 acttttgaat caaaatctgc atctgttact tttacgattg ccataaatgc caatcctcct
     8341 taattattat tatgatgttc aaattatatc agataacatg cttcatgtat ttctatttgc
     8401 tcaattttta tgcttatttc aaataacatg tttgaaatgt attcttgttg ctttacatta
     8461 ttttaactgc agctatttaa tttatcaaat tataatttat tttagtgttg caacggtaac
     8521 gccaaatcca ccttcgcttg gcataccacc tctaaagtca ctaacacttt tatgcttttt
     8581 caaatgttgt tgtacacctt tttgaagtgc acctgtacct ttaccatgaa tgatataaac
     8641 ttgttcgtaa ttacttaaaa cggcttgatc taaatattga tctagttcaa ttaaagcatc
     8701 ctcataacga tagcctcgta agtcaagttc agttttaatt gtttgacgat tttgacgtgt
     8761 aaccattttc gttggcttaa ctttttcttt ttgttttttc tctaaatctt caataggtaa
     8821 cttcatttta ataattccca tttgaacaat tgcttcttca tcattgacaa tttctaaaac
     8881 ttcacccttt tgaccgtaag ataatacttt tacttcatca ccagcaacaa ttttatcgta
     8941 tttttgcttt tgtacatttt gctttataga tttcgcttca taatgatcat ctaatcgttt
     9001 cttcttatca atcaattcat gttctttaac atctgcacct ttttgttcac gcaattgtct
     9061 taagtcttta ataatatcgt cagcttcttt tgttgcagcc ttaatcttct gatttgcttt
     9121 ttctttcgct tcctctatta gagacttttc ataattttgg aattgttgat actgcttaga
     9181 taaatcatca tgcacttgct ccgcttcttt tacaagacgg tccagttcta acctctgtgt
     9241 ctctacacgt ttgtaattac gctctaatga ttcaatcatt tcatttattt ctttttcatc
     9301 agtaccaatc atcgtcttag ccttattaat aatattcaaa ctaagaccta actttttaga
     9361 aatgtcaaaa gcatttgaac gacccggcac acccattaat aacttatacg ttggactcaa
     9421 agtatctaca tcaaattcta cactcgcatt cataacgcct tctcgattat aactatatgc
     9481 tttaagttca ggatagtgcg tcgttgccat tactagagaa ccaatttttc taacatgatc
     9541 taaaatgctc attgctaatg cagcaccttc actcggatct gtacctgcac ctaattcatc
     9601 aaataaaact aaactatgtt tgtctgcatg ctttaaaatt tcaactatat tcgtcatatg
     9661 agatgaaaaa gttgataatg attgttctat tgattgttca tctccgatat cgcaatatac
     9721 atttttaaat acacttaact gactaccatc aagtgtggga atcaacaatc ctgattgagc
     9781 cataacaata attaaaccta atgtttttaa tgttacagtt ttaccacctg tattcggtcc
     9841 tgtaataatt accgtttcaa tatcttccat aaattcgatg gtattagcta caacagtctc
     9901 acgatttaat aatggatggt atgctttagg taaatataca gtacggtcct ctttaaatat
     9961 cggctttgtt cctttaatac ttctactata tctcgctttt gcgattaaaa aatctaactg
    10021 acccatgact tgttctgcca caagtagtgc atctttgtcc gcagccacat aaccagttag
    10081 ttgcgttaaa atgcgttctt tttcaattgc ttcgtcatga cgtaatcgac taatttgatt
    10141 attcatttca acaactgatg atggctcaat atacaatgtt tgtcctgaag cagattgatc
    10201 atgtacaatc ccattaaaat cttgtcgata ttcagctttg acaggtataa cgtttctttc
    10261 attcctaact gttacaatag catctgataa ttttttctga tttgcttggc ttttaacaat
    10321 acggtccaaa ttttgtctaa tacgttgatt cgtgctagaa attttacttc taatcccttg
    10381 caattcataa ctcgcattat catataaatc atacgtatcg catgtttcat ttatttgttg
    10441 aaaaagatca gttaacacag gtaattgatt catcttgtca tctaatattg ggtatttaac
    10501 accttcatct tcttcaacca attgattata aaatgtcttg aattgatttt gtacttgaat
    10561 taatcttttt atcaagttaa gctctgatac atttaaaacg ccgccaatat cagcgcgatg
    10621 aatgaatgct gatactttag ataagccact caagcttggt aaacgatgct tattatagat
    10681 ttgagcaatc tcatccgttt cttccatttg aaaaacaacc gtttcaaaat tagtagctgg
    10741 catcatttga ttgacctttt ccaagcctaa gtcactaata gtttcattgg caacgagtga
    10801 ttttattttt tcaaattcta agacgtctaa tgttttttgt ctcataaaat ccctctattt
    10861 cttaagtttt gtattatttt caataaagtc tttaaaagct tcacgcgata atgcgttaat
    10921 cactctatct tttgttacaa atcctttttg cgcagttgct acgccataat tcataaaatc
    10981 taaatgattt gtatgatgcg catcagtgtt aatagttaat ttcacatttg gatatttacg
    11041 aacgatatca gcgctcagat ccagtcgatg tggattggca ttaatttcta atactgtatt
    11101 cgtttcttca gctaatgcca ttaattgttc aatattcggt ttataaccat ctcttctacc
    11161 tataatacgc cctgttggat gcgctatatg tcgcacgtat ggattgcgac atgcattagc
    11221 taatcgttcc ataatttgtt cttctgattg gttaaagctt tgatgaatag ctccaattac
    11281 ataatcaagt tgtgctaaaa tttcatcatc ataatccagc gagccatcag gtaatatatc
    11341 catttctgta cctgaataaa tatcaatttc actatattct ttatctaaag ccttaatttc
    11401 ttcgttttgt cttaaaagtc tttctacttg taagccatta gcaacacgta aactttgtga
    11461 atgatcagta attaccatga atttataacc ttttgcgata tttgcttcta ccatgtctcg
    11521 aatagaaaac gcaccatcac tatacgttgt atgcatatga atatcaccat taatatcatc
    11581 tattgtaatg atattactta gatctttatc aaattcgcta ccatcttctc gcatagcagg
    11641 tggtataaaa ttcacattaa aatgttcata tatcttggct tcactatcat attgaattaa
    11701 tgtaccatca gcttgttcaa ttccatattc acttactttt tcatcacgtg ctttagcaag
    11761 ttgtcgaatt cttatattat gttcttttga cccagtaaaa tgctgcaatg tatgataaaa
    11821 agcacttggt tcaattaatc gaaaatcgac accaatcgtt tcatcatcat acgctaattc
    11881 taatgaaact tttgtgttcc ccactgcaac ttcttttact ttattgggaa tatttaataa
    11941 ttgctgctgc actgcttttg ggttatcggt acttattatg aaatctaaat ctttgctcat
    12001 ttctttaaaa cgacggaagc ttcctgcaga tgaatattga tcgatataat ttaatgtatc
    12061 tatataatca atgatttctt gattaagtct tctcatttga tcaattggat atctatcttt
    12121 cttagcacca agttgtttca cagcttctaa tatgttttgt tccgttttct tagcaaatcc
    12181 gcttaattca ctaacttttc cattttcaca agcaacttga agtgacgctt tatcaacaat
    12241 attcaactct ttatatagct tagcaatttt cttgcttcca agtccttgaa ttttcaaaag
    12301 tggaataaga ccttccggaa cttcttcctg taattgctgt aaatactgag attcaccggt
    12361 ctcacggtaa tcattgatta cttctgcaac acctttacca atgcctttta actccgttac
    12421 atcagatatt tcatctaatg gtcgttcatc taattcaaga ctttgagctg cttttcgata
    12481 cgctgatatt ttaaaagtat tttccccttt taattccata taagtagcaa tttgttctaa
    12541 tagtttgata acatcttttt ttgtcataat aacactccat aaaaagaaga ccaggacgta
    12601 tcattaatat atacctttgt cctgacctct tatgataatt tataaattaa tggtgaaagc
    12661 cgataaatat gggatgtgaa aaatcaatga atgactcaca atagatattt taagctgatg
    12721 ttgtaatgct tcgttaggat ataatgccaa tagatataac gtaaagtgta agacaattat
    12781 cgtcataaac acaccaacta tcattcccat tgcacgactg aaaatatgaa tgttttgata
    12841 cgctattatt ttatcaaaag ttacgataat tagatataaa atgaacttac aaaacaatgt
    12901 aatcattaaa aaagctacaa tcgcttcaaa tcgattttgt agatgattaa aatgaaacgc
    12961 aaaagttgta ttaaatgctg ttgttttagg atatggaata aatacaatta atctttctac
    13021 aatagatttg taaaattgac tggcaatcca caatgataca atcgttgcac tcaaatgtat
    13081 catagataac caaaaacctc gtctgaatcc aacgatgaca aaatacacaa agaaaatgat
    13141 tatgataaaa tcaatgacca tttattgctc acgctgctgc aatttgtgaa tttgttgttt
    13201 caaacgtcga ttttcttctt ctagtagtac tttttcatgc ataatattca cagcagttag
    13261 tattgctttt cttgaagtat ctaaacctgc tgctttatac cctaattctt ttattttatc
    13321 atcaactaaa tgtgctacat atcgtatgtg ctctgggtta tcttccccaa caattgtaaa
    13381 aagctgatca ttaattgata catttacctt gtttttaaac tgtgtcattt ataatttctc
    13441 ctgatccttt ttttaaaatc taaattcacg ttataaaata tgactggata gtttgtctga
    13501 atttgatact aatattgtta tattgcaatt atgataaaac aacaacacaa tctctataga
    13561 tgacttaatg ttctttttat aatgaaataa tgtaaagaat tttctattca atactttatc
    13621 atgtttaaat tgtgtcacta taacattttc ataaacatta tacatgacca ctatgtattt
    13681 tgtaagtatc cgcaattaat tctttacaac atacataaat gtttctgacg ttattatcat
    13741 ttatgatatg attatttttg ctaaagacaa tgaaatttta tgaaaggatt tacacaaatg
    13801 gcgaatatcg tttttaaatt gtcggataaa gacataacga cattaatgtc acgcatttct
    13861 tttgatactg agaatttacc tcaaggtatg aaagcacgtg caaagtatca aaatacaact
    13921 gtaaatattt accaatcagg taaagtcatg tttcaaggta atcatgcaga agctgtgtct
    13981 gaagaacttc taccgcagca ttctcaattg aatactaata aaactaaaaa gaaaaatatg
    14041 gctaattctt ttcttgaaca aactttaatg tatgatcaat ttaactgtat tggtagtgat
    14101 gaggctggaa gtggcgatta ctttggtccg ttgactgtgt gtgctgcttt cgttaccaaa
    14161 gagcatgtcc ctattttaaa aactttgggt gtagatgatt caaaaaaatt aaccgatacc
    14221 aaaattgtag aacttgctga gcaattggtt actttcatac cacattcatt attaacacta
    14281 cacaatgaaa aatataatat tcagcaagca aaaggctgga ctcaagtaaa aatgaaagct
    14341 gcccttcata atgaagccat caaaaatgta cttgaaaaaa ttgattcgtc tcaattagac
    14401 tatatcgtca ttgatcagtt cgcaaaacgc gaagtgtata gccattacgc attatcagat
    14461 ataccacttc caaagaaaac aaaatttgaa acaaaaggag aatcgaagtc attagccatt
    14521 gcagtggcaa gcataatttc tcgatacgca tttgttacat atatggatca aatatcaaaa
    14581 aatattaata tgacgattcc taaaggtgcc ggtgctaaag tcgatgtaat cgccgccaaa
    14641 attataaaaa aatacggtct ttcacgttta gataccattt caaaaaagca ttttaaaaat
    14701 cgtgaaaaag cccaaaaaat tttaaagcct ctttgatttt taatcattag aggcttttat
    14761 gcagttgtgc gaagcatcat ctgtattccg ctgttcctct ttgacttttt aaatgcattg
    14821 gaaccaaatt ggtgaaccca aaaataaaac cccatgttag ggatatcaat ctacttcaga
    14881 tatccttata catggggttt aaatcattat ctaataacag caccttgttc aattaatgct
    14941 gcttcaattt ccgcttgtac ttttgaaacg cgctcatctg tcaatgtttc ttctgtgtct
    15001 aaataattta aacgtattgc aattgatttt ttaccttttt ctaaatgttc gccctgatat
    15061 acatcaaaga caagtgtatc ttttaatata ttgccaccat gtgcatgaat cgttgataat
    15121 aaatcagctg ctggaatatt ttgatctact tctaatgcaa tgtcacgaga catgcctggg
    15181 aatctcggaa ttggctggta attaatgtaa cctaccgaca cagccattaa tgcatcaaaa
    15241 ttcaactcaa aaacatacgt acgttttaaa tcattatcag ctgctaatat tggatgtaat
    15301 tcaccaataa aaccaacaac tttattctct aataagattt cagcagtacg acctggatgt
    15361 aatccatcaa tatcagcacg gcgataacta aattcaagat ttaacttttc agatactcga
    15421 tccacgacac cttttgctaa atagaaatca accgtttctt tcttatcttg ccattgattg
    15481 actacataat ctccagttaa aataccactt aaatattcaa cttgatctgg tagttcacct
    15541 tctccattag caaagaagac attgccgatt tcaaataatt ttacatcttt atttttgcgt
    15601 gccacattat atgatgccgc ttcgattaaa tgtggtaata aactttgacg taatgacgca
    15661 tgcgcttcac tcattggcat caataaatca attgtttgac gctgttgcat cgaaaatgca
    15721 gtagcatctt ctttagaaac taacgaatac gtaatagctt ggtctaatcc agcaccttct
    15781 aacacttctt taaccattct agttttatat tggcgatcag ttagctgacc actagtaact
    15841 ttatcgaaga caggtaacgt tgatggaata tcgtcgtatc catatatacg tgcaacttct
    15901 tcaattaaat cttctttaat tgtaatatct ttacgacgtg atggtactag cactgtaata
    15961 acatcatcat ttatttctgt atcaaaccct agttgattaa aaatagtaac aatatcattt
    16021 tgtgacaaat caaatccaat agtgcgatta attttatcag cagtgatgtc gattggtgta
    16081 ataaatgcac caagttctcc tgaagacact ctatctttta gcacttttcc gtttgcataa
    16141 gtttgtaata aataacatgc acggtcgact gcttcatcta caaattcagt agctattcct
    16201 ttttcaaaac gactagatga ttcactgcgt aaatttaaac gtcttgatgt atgacgaatt
    16261 gaaactggat caaaaatagc accttcaatc actatatttg atgtttgttc tttaacttct
    16321 gaaaaatcgc cacccataac accagctaat gcaattggag tttgtccatt agtaatgaca
    16381 atatcgctcg ttaataattc acgttctgta tcatctaatg ttgtcatttt ttcgccttca
    16441 ttagcttgac gaacaacaat ttgttgtgaa ccaatcgcat cttgatcaaa catgtgcaat
    16501 ggttgaccgt attctaataa cacataattt gaaatgtcaa caacattatt aataggacgt
    16561 atacccgctt ttattaagcg tgcttgcatc caaattggcg agggttcaat tgtcacgtcg
    16621 tgaacaacac gtgcactata atatggtact ttatcttcat tttcaattgt cacagtcagt
    16681 tcatcatttg cagataaatc aagctcattt gatgttgtct ctggcttagt catttttgta
    16741 ttatataatg ctgcaacttc ataagcagta cctatcatac ttaaagcatc tgcacgattc
    16801 ggcgttaaat caaattccat tacttgatca tctaaatata aagcttgtaa ggcatctgtt
    16861 cctggaactt gggcttcact aaatacataa atgcctgatt caaaactttt cggtatatag
    16921 ttacttgaaa taccaatttc ttgtaacgaa caaatcatac cttctgaacg ttcaccgcgt
    16981 aatttggcac gcttaatttt aataccacca ggcaatctgc cacctacttt agcaacaatg
    17041 acatattgtc ctgcatcaac gttcggtgca ccacaaacga tttgtacagg ttcgtcttct
    17101 ccgatatcaa cttggcaaac atttaattta tcagcatcag gatgtttctc ttttgactta
    17161 acgaagccga caactaaatt tttgatatct tttgtgtagt caattaaatc atccacttca
    17221 atacctgtgc gcgtaatacg ttctgccaaa ttacttacag aatcatcgat tgttacatat
    17281 tctttcaacc attcatttga tatcaacatt acatgtcacc tctatcttct accgctttaa
    17341 attgatctaa aaatctcaca tcattagtat agaaatgacg aatatcttct ataccatatt
    17401 tcaacattgc aatacggtct ggtcccatac caaatgcaaa tccagagtac tctgaagaat
    17461 caaaaccagc catttctaat acattaggat gtaccattcc agcacctaaa atttcaatcc
    17521 atcctgtgtg tttacacaca ttacaacctt ttcctttaca tttaaaacat gacacatcaa
    17581 cttctacaga aggttcagtg aatgggaagt aacttggacg taaacgaatt tcacgatcag
    17641 caccaaataa tttcttagct aacaattcta aagtaccttt caaatcactc attttaacgt
    17701 ttttatcaac aactaatcct tcgatttgtg taaattgatg actatgtgtc gcatcatcag
    17761 agtcacgacg atacacttta ccagggcaaa taattttaac tggaccttga ccatgacgtg
    17821 attccatcgt acgtgcctgc actggtgatg tatgcgtacg taataaaatt tcatccgtaa
    17881 tatagaaact atcttgcata tcacgtgcag ggtgtgattt aggtaaattc agcatttcga
    17941 agttataatg atcttgttca acttcatatc cattcacaat ttcataacct aaacctaaga
    18001 ataagtcttc aatttcttct attgtacgtg ttaatggatg ctttgaaccg atttcaatat
    18061 gacgacctgg taatgataca tcaattgttt cttcagccaa ttgcttattt aatttttctt
    18121 taactaacat ctgttgtctt tcatctaatt cattttgaat tgtttgacgc aattcattca
    18181 ctttttgacc aaacgcaggt ttatcttcat tcggcaaatc cttcatcaat ttcattagtc
    18241 cgctaactga ccctttttta cctaagtatt tcactttaac ttcttgcagt gcacgttcat
    18301 catttgcttc attaatatct acaagcgctt gttgttttaa ctctgacatt gtttgttgtt
    18361 cagacatatg tcactgacct ccctattcga actattcatt ttctatttat catatttgtt
    18421 gtttaactaa aaaaagactt tcatcctctt aaaaaataag ggacgaaagt catttccgtg
    18481 gtaccaccca aatttatgcc taagcataca cttgtttaat attgataacg gattgtcttt
    18541 ccgacttaaa tctctttaag taaccaaaaa ggtgaaaaga ctttaaataa ttggaataac
    18601 ttgcagtcac gattattctc cctaaaacaa ttatttatat aaatgtccat gcctttttat
    18661 tggtttatat tcaattgtgt atgcattcac gtagaaatgt tctgttaaac aattaattat
    18721 aattatatcg gaaaactttc aacacggtca acctttcaaa tgataaagta aaatactacc
    18781 tgcaatcgct acatttaaac tttcagcttt accataaata ggtatgatta aattttgtgt
    18841 agtatgtgct aataattcag gattaacacc ttctccctca ttacctaata gtaatgcaaa
    18901 agaatcactt gaagtaactt ctttgtatgc cactgcgttt tcaagtgctg taccataaac
    18961 aggaccatta aattgagtaa taaacgtatc gagatcttgt gtcataactg gcaaatggaa
    19021 aacactacct tgactcgctc gcaacacttt atcttgataa ggatctgtcg tacccttctc
    19081 cattattaca gcatccattc cagcagcatc cgcagttcta attaatgtgc ctaaatttcc
    19141 aggatcttga acacgatcga tgagcaatac ttgttgtgct ttagaaatat cataatgcgg
    19201 cttctcaatg attgcgaaaa acccttgtgg tgtcactgta cctgataaag attcagcaac
    19261 tttcatgttt atttcaaaaa cttcttgcgc gtatgcgata atttgctgat ctaatcttgc
    19321 cggttcaatt gcaaataatt gtgtaattac aattccactt tgataagctt cttcaattaa
    19381 atgcacacct tcaattaaag ctaatccagt tttatccctc tcacgtttct tttttagctt
    19441 gttcgcttgt ttaattctat tattttgtgc agaagtaatt tgttccattg atagctcctc
    19501 gctttatttt taaaaataaa aatattaatc attaataaga tgaaaacatt tgattgtata
    19561 gttaatatta attaatcgct tttatcactc ataatatttc aaattgtata aatttctttt
    19621 atcgatacta ctactataaa tcatacgccc caaaatatca ttattaattc ttttcttctt
    19681 caaaataaat caaaatgata taattgatga ttattttcaa agcacattca aatcaaacta
    19741 tgttttagca atttgttgtt agcatgtttg tgttcattaa aaaaacgacc atcatcggta
    19801 tcatgtatgg tcgttacaaa agctaacaat accaattgtc ataacaagta ctgcaacctc
    19861 tttaaattca attatttcat gtaactatag cctatatcat atgtaattac tttgttattt
    19921 ataatcgggc tactttcatc ttcattttta cttctaacat gtttatgcgc tgctttaaag
    19981 acatcagatt ttaaccaatc cgtaaaagct tgctttgatt tccaaactgt taaaattttc
    20041 acttcatcaa aatcttcttg ttctaaagtt tgtgtaacaa acatgccatc aaagccttct
    20101 aatgtttcaa tcccatgtct cgtgtaaaat cgttctataa tatcttttgc tgttcctttt
    20161 gttaacgtca gcctattttc tgccataaat ttcataatta tcctcttttc tgtttaactt
    20221 accttaatta tttttgcgac aacaacaatt cttttcgtcg tttcactata tgcatcttcg
    20281 cacgttgata aagtcattat tctatctttt accgttacat taacatctga attaattaca
    20341 gatttacgtt ttgtctcatc taaaaattgt tgataatctt gatcattttc aaaatctgta
    20401 cgtatgtaat tatctttagt agtagtttta tatgcactaa atacttgcaa ttgatattta
    20461 ccatatttat tgtcaaattc aattatcttg tgtttttcat aaaacgattg ctttaaataa
    20521 tcttctaaca catcaaacat cgtattatca ccgacatggt gcccgtataa aatagtatta
    20581 tgatttaaat tcttcaattc atttctaaaa tccataaaaa tactaccttt acgtcgatgt
    20641 tctcgctcaa aatctaaatt taaataatcg tgatttgtct taccttgtag tactggataa
    20701 tttaatgatg ttcctgataa ttttatccat ccaacaatgt ctttatttat tttttcaagt
    20761 gattcaaatt gtggtctcac atgttcttga tgtttgctca tcagcatttg aaatttttgt
    20821 tgtaatttct cataatttgc gcgttcttgc ttgtcttcaa tatatgtttg aacaattttg
    20881 taaccaaaaa tgataataat tacaaccaat aaaatttgta caatagttaa aaatcgcttc
    20941 attctcataa aaatcctctt ttattaacga cgtttcttca gtcatcacta aaccagttgt
    21001 tgtaccgttt tagattcgat ttcgttgact ttgacaaatt aagtaaatta gcattggacc
    21061 accgacaatc attaaaatag cattggctgg aatttctaaa ggaggctgta tcactcgtcc
    21121 taataaatca gccactaaca atagccatgc accaataact gtagaaaacg gaataagtac
    21181 tctgtaattg cccccaacta gctttctaac cacatgtggc acaataatac ctaaaaaggc
    21241 tagttgtcca acaatcgcaa cagttgcact tgctaaaaat actgctaata aacctgttaa
    21301 ccatctgtaa cgatcaatat taaaaccgat acttcgcgct tgtatgtcgt ctaaatttag
    21361 taaattcaat ttaggggaca atagtaatgt taatattaat cccaataatg ctgatactgc
    21421 taatatgtat acgtcgctcc atattttcat tgttaagcct tgaggaattt tcattaaagg
    21481 gttttgagtt aaaatttcta aaacaccatt taataatacg aataacgcaa cacctactaa
    21541 tatcatactt acagcattga atctaaattt agaatgcaac aatataatta ttaaaaatgg
    21601 tattaaacct ccaataaaac ttaataatgg taagtaaaag tacaattgtg gaataaacaa
    21661 catacaaagt gctctcatta taagtgcacc tgaggaaacg ccaatgatat tcgcctctgc
    21721 caaaggattt tgtagtgctg cttgtaataa tgctccagaa actgctaaca ttgcgccaac
    21781 catcaatgca attaatatac gtggcaatcg caaatcaatg attgaatcca ctgcttcatt
    21841 gctaccagtt gtaaattttg taaataggtc attaaatgac aatttaattg taccggttac
    21901 aaacgaaata taagcagttg cgattaaaat gactaacaaa cataaaaata gtagtttctt
    21961 tttatttttt atcatcatat caactcacta ttttttatcc ttataaaata aatcatataa
    22021 ttgagtcatt gccttatcag catcaacatt tgctgtaata ccgaatggca cttcctctaa
    22081 gtcataaaca tgattatttt tcacagcttt aaaatgtttc caaatatcat tttgtttaaa
    22141 ttctttttga aacattttct taacttcttc aggcattccg tgtggtaatc gtaaaataat
    22201 atctggattg atattcaaca aattttcagt attagacgaa atatattgac gatctttcac
    22261 tttaataaca ttttctccac ctgctatttt aactaaatca ccaatatatg atttatcagt
    22321 tgctactaaa tagctacccg gtacacccat taatattaat actttgggat gtttcttttg
    22381 tttagctgct ttattttcaa ttttttgctt tacagaattt aaatggtcat ttaattcttt
    22441 tgcttgtgct ttacgattaa attgatcacc taattgtgta atcgactttt gcatcccttt
    22501 taaactatca aaatcataaa agtagccttt catatttaat tgtttgtaaa atggttgcat
    22561 ttcatcttta atcgttgaca cactcaaaac atgtgttggt tttaactttt taacagcttc
    22621 aacattcggc tccattggtt gaccaatttc cggtacatct ttataacgat taggcaatgt
    22681 cttatatgac gtgggtttgc cgacaattgg taaatccaat ttgtccaatg tcattgtcaa
    22741 tgcaacagtt gttggtacga ttctgaattc gccggattta gttgattctt gagaactgga
    22801 agattgacag ctggttaaga taacgacgct tatcactaaa atggttaaat actttatgat
    22861 tctcaaactt ggttacctct ctttctgctg tataaatgtg ccgaaataag taaaatagtt
    22921 gctaacacaa tgccagctgc aaataacata tattgctttt tatgatttgg ttcatcaagt
    22981 tctttaatat cactagataa ttgactacta ttcttgctat catcatctct attaaaagct
    23041 ggtaacgctt tttctttttc aactactttt ttcttttcct tttttgattt ttcatcttta
    23101 tgtttcggtt gctctttgtg attattttca acctttttta ttgttggagg gctattttca
    23161 acttttaatg tacctgatgc tttaggtcgc tcattttcaa tctttttatt actgtcttgt
    23221 tgcactttat ctttttgtac aggaatatca tttaatcctt tttcagtaaa aaggctagta
    23281 cgtctttcct tagtatctga tttttgacta tcttttttgt catcaatatc ttttttaagc
    23341 tgatcttgat tattttcttg attttgatgt tttgaaggca cttcttttgc atcatcatgt
    23401 tgtacttttt tctctttaaa atgaatagaa tacaccattt ctttgttatc tagatttttc
    23461 ttttcagcca acgttttaac atgcatattg attttatcca tatgctgttc aattaaaatt
    23521 cttactattg tgtcatcctt ttcatctttt ataacaaaag gaaattcttc atttttgtca
    23581 ttttgaaaaa caagttgttc tttatctatt tgacctttta gctttacttg caagtaaatt
    23641 ttaccagtca tatctgaaat caattgcgcc ttttcgtcaa ctagttgttg ttcctcagtt
    23701 acatttttat tttcttgatt aaaaattagt aactctaacg ctttattctc ttttatcttt
    23761 tcatcaaaaa ttgatgtagt taattctgtt ttatcttttg cttcaggttg attaaatttg
    23821 gctgatggat attttatctc atcagccaac acgctaccaa tcattgttgt atatgtaagt
    23881 atacctaagc taataatagc tataatagat tttgtagcaa tttgtttaac atttctcatt
    23941 attccacatt gcctttagat ttttttctaa caaacaatgt gattgcgatt aataatgcta
    24001 aggatgcaac tggtattgca tatatatatg caggtgtacc agcatttgtt tgtggatttt
    24061 ctactttgtc tttattcgaa ctattagatt cactttgacc agtagtcgtt ccatcagatc
    24121 ctttgtcact acctgaagca gaatttttat catctttacc tggtgcatta gcacctgcta
    24181 catcagttgg tccattaaat ttatatgtaa tgttgtaatg atggtcatat ttgaatggct
    24241 ttccatttac tttttcatcg atataaacgt caatttttcc atctatttta ccgttcaact
    24301 tacttacttc aaattcagaa gtgcgttcat ctttggcagt gtttttacta ataatatttt
    24361 ctttatgtcc ttcgatactc attccagtaa tccaatgact gtggttgaca gttatttgaa
    24421 catacaattt accatttttc ttaatgtact ttgccggttt attaaaatag tcattagcaa
    24481 ttgacgtgtc attggtattg tatttgtaaa cctcataatt caaagtaccg ctatctgcgg
    24541 catttgcaga attactgaat gtcgcgatga tgataattaa cgctaaaatc gttgtattaa
    24601 aaacttttaa aatatttttc aaaacataat cctccttttt atgattgctt ttaagtcttt
    24661 agtaaaatca taaataataa tgattatcat tgtcaatatt tattttataa tcaatttatt
    24721 attgttatac gaaaatagat gtgctagtat aattgataac cattatcaat tgcaatggtt
    24781 aatcatctca tataacaaca cataatttgt atccttagga ggaaaacaac atgacaaaac
    24841 attatttaaa cagtaagtat caatcagaac aacgttcatc agctatgaaa aagattacaa
    24901 tgggtacagc atctatcatt ttaggttccc ttgtatacat aggcgcagac agccaacaag
    24961 tcaatgcggc aacagaagct acgaacgcaa ctaataatca aagcacacaa gtttctcaag
    25021 caacatcaca accaattaat ttccaagtgc aaaaagatgg ctcttcagag aagtcacaca
    25081 tggatgacta tatgcaacac cctggtaaag taattaaaca aaataataaa tattatttcc
    25141 aaaccgtgtt aaacaatgca tcattctgga aagaatacaa attttacaat gcaaacaatc
    25201 aagaattagc aacaactgtt gttaacgata ataaaaaagc ggatactaga acaatcaatg
    25261 ttgcagttga acctggatat aagagcttaa ctactaaagt acatattgtc gtgccacaaa
    25321 ttaattacaa tcatagatat actacgcatt tggaatttga aaaagcaatt cctacattag
    25381 ctgacgcagc aaaaccaaac aatgttaaac cggttcaacc aaaaccagct caacctaaaa
    25441 cacctactga gcaaactaaa ccagttcaac ctaaagttga aaaagttaaa cctactgtaa
    25501 ctacaacaag caaagttgaa gacaatcact ctactaaagt tgtaagtact gacacaacaa
    25561 aagatcaaac taaaacacaa actgctcata cagttaaaac agcacaaact gctcaagaac
    25621 aaaataaagt tcaaacacct gttaaagatg ttgcaacagc gaaatctgaa agcaacaatc
    25681 aagctgtaag tgataataaa tcacaacaaa ctaacaaagt tacaaaacat aacgaaacgc
    25741 ctaaacaagc atctaaagct aaagaattac caaaaactgg tttaacttca gttgataact
    25801 ttattagcac agttgccttc gcaacacttg cccttttagg ttcattatct ttattacttt
    25861 tcaaaagaaa agaatctaaa taaatcatcg tcacactcat aacttaatat attttttatt
    25921 ttaaatttta tttaacctat gtcatagata tttcataatc tataacatag gttatttttt
    25981 tataaaataa cgttgcaatt aactaacatt tcaatgtaca atacaagtaa tcaattgata
    26041 atgattatca gttgataata tacaattagg agttgtttct acaacatgaa caaacagcaa
    26101 aaagaattta aatcatttta ttcaattaga aagtcatcac taggcgttgc atctgtagca
    26161 attagtacac ttttattatt aatgtcaaat ggcgaagcac aagcagcagc tgaagaaaca
    26221 ggtggtacaa atacagaagc acaaccaaaa actgaagcag ttgcaagtcc aacaacaaca
    26281 tctgaaaaag ctccagaaac taaaccagta gctaatgctg tctcagtatc taataaagaa
    26341 gttgaggccc ctacttctga aacaaaagaa gctaaagaag ttaaagaagt taaagcccct
    26401 aaggaaacaa aagaagttaa accagcagca aaagccacta acaatacata tcctattttg
    26461 aatcaggaac ttagagaagc gattaaaaac cctgcaataa aagacaaaga tcatagcgca
    26521 ccaaactctc gtccaattga ttttgaaatg aaaaagaaag atggaactca acagttttat
    26581 cattatgcaa gttctgttaa acctgctaga gttattttca ctgattcaaa accagaaatt
    26641 gaattaggat tacaatcagg tcaattttgg agaaaatttg aagtttatga aggtgacaaa
    26701 aagttgccaa ttaaattagt atcatacgat actgttaaag attatgctta cattcgcttc
    26761 tctgtatcaa acggaacaaa agctgttaaa attgttagtt caacacactt caataacaaa
    26821 gaagaaaaat acgattacac attaatggaa ttcgcacaac caatttataa cagtgcagat
    26881 aaattcaaaa ctgaagaaga ttataaagct gaaaaattat tagcgccata taaaaaagcg
    26941 aaaacactag aaagacaagt ttatgaatta aataaaattc aagataaact tcctgaaaaa
    27001 ttaaaggctg agtacaagaa gaaattagag gatacaaaga aagctttaga tgagcaagtg
    27061 aaatcagcta ttactgaatt ccaaaatgta caaccaacaa atgaaaaaat gactgattta
    27121 caagatacaa aatatgttgt ttatgaaagt gttgagaata acgaatctat gatggatact
    27181 tttgttaaac accctattaa aacaggtatg cttaacggca aaaaatatat ggtcatggaa
    27241 actactaatg acgattactg gaaagatttc atggttgaag gtcaacgtgt tagaactata
    27301 agcaaagatg ctaaaaataa tactagaaca attattttcc catatgttga aggtaaaact
    27361 ctatatgatg ctatcgttaa agttcacgta aaaacgattg attatgatgg acaataccat
    27421 gtcagaatcg ttgataaaga agcatttaca aaagccaata ccgataaatc taacaaaaaa
    27481 gaacaacaag ataactcagc taagaaggaa gctactccag ctacgcctag caaaccaaca
    27541 ccatcacctg ttgaaaaaga atcacaaaaa caagacagcc aaaaagatga caataaacaa
    27601 ttaccaagtg ttgaaaaaga aaatgacgca tctagtgagt caggtaaaga caaaacgcct
    27661 gctacaaaac caactaaagg tgaagtagaa tcaagtagta caactccaac taaggtagta
    27721 tctacgactc aaaatgttgc aaaaccaaca actgcttcat caaaaacaac aaaagatgtt
    27781 gttcaaactt cagcaggttc tagcgaagca aaagatagtg ctccattaca aaaagcaaac
    27841 attaaaaaca caaatgatgg acacactcaa agccaaaaca ataaaaatac acaagaaaat
    27901 aaagcaaaat cattaccaca aactggtgaa gaatcaaata aagatatgac attaccatta
    27961 atggcattat tagctttaag tagcatcgtt gcattcgtat tacctagaaa acgtaaaaac
    28021 taataaatcg tctttatatt taattattaa attaacaaat tttaattggc ggatgaggta
    28081 tccagttacc tcgttcgcca attatttttc gcaatataaa aagtcccact taaaacaatc
    28141 attttaagcg ggacttttta tattgagtaa ctaaaattat ttagctgcta cttcttcgcc
    28201 attgtaagaa ccacagtttt tacatacacg gtgtgataat ttgtattcgc cacagtttgg
    28261 gcattcagtc atacctggta ctgaaatttt gaaatgcgta cgacgtttgt tttttctagt
    28321 tttagaagtt cttctttttg gtactgccat gatatatcct ccttagatta taaacgaaaa
    28381 atactaaatg ttagtttaat taacaacatt atatcattaa ttaaactact tattgctctt
    28441 tatcatataa ttgttgtaat ttttgaagcc ttggatcaac ttgtcgtgat tctgaatcat
    28501 cttgttcttg ctgtttagca agctcatcta attgatcctc atcgattact tcccaaccat
    28561 tacctactgt caacatttgg tcactttgct ctgaataagc tctcattggt ttctcaataa
    28621 taactatatc ctcgacaata tcctgaagat taaccatacc atctttaata atgtgatagt
    28681 gttcatctac atcatcttga tcatcgttat actgattgta cccttctaaa tcaaatactt
    28741 ctgtagtagt tacatctagt gggactttta ctggtacaag agtacgtgca caaggcattg
    28801 tatacgttcc agtaatgtga atatccgcaa cgacttctgt tgacttaatg gttaactgac
    28861 cttggattgt aattggagat aaatcaatta aatctaatga ttcttttaaa ttgtcaaaac
    28921 tcaccgtttg atcaaattca aatggcttac cttgatattt ccttaattgc gtaattgacc
    28981 atttcatatg gcttcacctc taacaagcac aaaatttatt ttaactttct aagaacaagt
    29041 tgtcaagatt ttttcttaac atatcatgtt ctgttacaat attaataatg aataccgaaa
    29101 ggacgaattg caaatgaaaa gcgttggctt aatcacagaa tataatccct ttcataatgg
    29161 gcatcaatat catattaatc aatctaaaaa acttacaaat gctgacgtta ctattgcaat
    29221 aatgagtggt aactttgtca tgcgtggcga accagcaatc tataataagt ttactcgtgc
    29281 aaaaatggca ttatcaacag ctgatttagt tatcgaacta ccagcaactg ccagtttatc
    29341 atctggcgat cattttgccg aactagcagt taaagtcgca gattatatga gtgtcgatac
    29401 aattgcattt ggtagtgaaa ataatgatat caaaacatta aagcaattag cacacagcat
    29461 taatgaaatt gaacaatctg aatccttttc acaaaaagta aaagaaggta aaagctaccc
    29521 tcgtatcatt agcgaattat tagagcatca tgaagcacta gcaagtccta acaacatact
    29581 tggtattagt tacctgaaag caattgctaa aaatgctaaa aacatcaatg caatttctat
    29641 caaacgagaa aatgctcaac atcatgattc attaattcaa caccatcagt ttgcaagtgg
    29701 tacatctatt agaacatcaa tcattagtca agatgatcat tggcatcatg tggtacctaa
    29761 agatattcaa cacttgtacg taacacctca tataacatta aatcaaatat ttccatattt
    29821 gaaatatcaa atcatagcta tgactacaga ttcattaaaa aatatttata ctgttactga
    29881 aggttttgaa aatcgtttga aatctaacat ttatgaagca actgattttc atcattttgt
    29941 aaaattacta aaaacaaagc gttatacata tacgcatatc caaagactgt taatgaatgt
    30001 attattaaac attaaaccta cagatgttac gagtaacatt cacgcagtta aagtattagc
    30061 gatgaacgac cgtggcagac aatatttaaa gcatttaaaa acagcgttcc cagaaagaca
    30121 atatataacc aatattaaca aaagcaatgc acattacttc acaaatgaaa tcaaagcaac
    30181 acacatatac aatgcaataa gcgggcaaca acaaacagat ttcaacacgc ctgtcataca
    30241 gcaatatcgt taacaaatga aaacagtatt ttaggattgt aaaccatgat aaacctaaaa
    30301 tactgttatt tttattactt aaatttcttc ttcaatgcct tttcaacata aggtggaacg
    30361 aattcagaaa tatctgctcg ataagctgca acttctttaa caatacttga acttataaat
    30421 gaataattag tactagacat catatataac gtttcaattt cattgttcaa ctttttattc
    30481 attgaagtta agcgtaattc atattcaaaa tcactgactg ctcttaaacc acgtatgatt
    30541 gttttagctc ctacttgttc acaataatcg actagtaaac cactaaattg atgaaccttg
    30601 acattaggta aatgtttaac agattgttca attaaatcca tacgctcttc taaactaaac
    30661 gtaccttctt ttttactatt tttaagaaca cagacatgaa tttcatcaaa tctatctgta
    30721 cttctctcaa taatgtctaa atgaccataa gtaatggggt caaaactacc cggaatgacc
    30781 gctattgtat gttccatgct attctccctt ttctaataac aatgtgtctg tcaacccata
    30841 atggtaacgt ttaatcatat taaacggttg ataatctatt tcttcatgat tgctaaattc
    30901 acaaacgatg ataccatttt ctttcaataa attaaactct gaaattagtt ttaaagcttt
    30961 atcaatgaga cctttattat aaggtggatc taagaaaatg acatcaaatt gaatatcacg
    31021 ttttgacaat gcttttaaag ctctatctgc attattttta taaacttcag attgtgcctc
    31081 taaatccaaa ttcgcaagat ttgatttaat aacttttaca gctttaaaat tttgatcaac
    31141 aaagattacc ttatccatac ctcgagagag tgcttctatt ccaagcgccc cgcttcctgc
    31201 aaataaatct aaacctatac ctgacacatc atataaacta ttaaagatac cttctttaac
    31261 tttatccata gttggtctcg tattacggcc ttccatactt tctaaagctt tacttttatg
    31321 tttacctgca atgacgcgca tgttgttcac acttccaatt catttagtta tttaatataa
    31381 tttattgaga aaaaggagaa tgataaacca atgaaacaaa catttattac acttggtgaa
    31441 ggtctaacag atttgttcga attcatgacg atgattgaat ataaccatca acgtattgat
    31501 aaaattatct attttcattc accacaagct gaaaataaaa agtcatctgt agcaatcatt
    31561 atgaacccta caactggcaa tcatttccaa gcattttata tcatgataaa cgctattaaa
    31621 tatccatatc cagattcaaa taaaaagttt caaatgataa atgattgtgc tgaaaaattc
    31681 gacataccaa ttttaggtat cgatgtacag ccccctcaag catttcatga tttatcgtta
    31741 tattataatt atttaattag tgtgttaagg ctccaaaaat ggataccaga acttcaataa
    31801 tattaattat atatttcgtg tttctctttt tcgtaagttt tctttaagta tttatatggt
    31861 gagccatcaa tgtgtctaac gtatttaagt tgcattagtt tatgtacaat ttgatcaaca
    31921 tcttgctcat tcacatacat aattacaaat ttacgatctc tatttgaatg aacgatatgt
    31981 ccatattttc ggatttgtcg ttcatgtttc atatgtttta aataaactac aatactagtt
    32041 cttgggatta aattcattta aacctcactc catttatttt cagcattaat catatcatgt
    32101 ctttattaat tttaaaatat taagctacct taattatgtc ttatgtaatt gctcgataaa
    32161 tgtatttaga ttatattaaa catcattata ttaattcaat tctcaagagc atcactatat
    32221 gattaaccac acatttcgct gtagtcagaa ctatgaattt gttaagctat aaaagtaaac
    32281 gaggaggaat tttttatgaa aaaagtaaac agaaacattt taaatgcggt actagtcggt
    32341 gctggatttt taagtagtct attaatgatc aataaaaata aagtgattac taaaaagcaa
    32401 accatccctg cttttttcaa aggcaatgca ccatatatat ttgcgcatcg cggtggtatg
    32461 gctttaagac cagagcaaac gcaacttgca tttgactatg ctaaacaatt aggcgttgat
    32521 ggctttgaaa cagatgtgag actcacaaaa gatcaacagc ttatcgtttt tcatgacgca
    32581 actgttgata gaacgacgaa cggctctgga aaagttagtg cccatacatt agcagaatta
    32641 aagaaattag acgcagctta tcacttcaaa gatatcaatg gactaacacc atatcgtggt
    32701 catgcacaca cagctatact aaccttcgat gagttgctga agcaatatcc tgatatgtat
    32761 ataaacgtag acttaaaaga tgctcctgaa tcctatgaag gctcaatcgc accacaaatt
    32821 atgtttgata caattgcaga aaatcaagca tttgaccgcg ttcttgtgac aagtttttat
    32881 aaagaacaaa tcgttcgttt taataaaatt gcacaaggat ccgtcgcaat tggtgctagc
    32941 caacaggaag ttacagaagc atttttaaaa tatcatttat taggtggtag gtactaccaa
    33001 ccattagcac aaacgttcca aatgcctact cattttaaag gcattgattt gacttcatct
    33061 cgttttatca aatggttaaa tgatatgaat atcattccag gctattatgg cgtaaatagt
    33121 ataaatctga tgaatgattt ataccaaaaa ggtgcacaca cgattgtgac agatcgacca
    33181 gatttagcac aacaattcaa acaaacaatc ccgaataaat aaaaacatcg agtcagatgc
    33241 tagttagtgg tttcaataac cacctttttc tgactcgatg ttttttataa tgaacaatta
    33301 caggatccgc ccgtcgcaca accactatgt gatgtttgaa agaatggatt acctgtttct
    33361 attttgacat gttctgaaac agcgtacgca attttcgtaa caacttcatc tattaaatgt
    33421 tgaagtgcca tttccctagc tttgtaatgc atcacaacgt caagcatctc atatgctctt
    33481 ttgcgttgtc ttgtctctaa cataactttc ttataatcag gatgatattt cccgaatcgc
    33541 attacttcat catatttctc ttttgatttt aaaaatgatt gataaagtaa atgtgcttcg
    33601 tcattgtttt cgagcgcctg tttagcttgc tcaaatgatg cataaatatc tgatgcaact
    33661 atcatatcag ctagttcatc aatatcatct aatatcgcta gtgaagcttc attaatcata
    33721 aatacccctc attgttattt ttctaagaaa attagtgaat aataatctct cattacatct
    33781 ccacccatct catcgtattt tgaatttaat agacgagatc gatgtatatc tgaattcatc
    33841 caactatgga ttaatgttgg tacatcatta aacgcataac caacattttg agcagttgtt
    33901 ttataagtaa cgtgattttt atctaattgc cctcttaatg cgtcctctgt aaattcaaca
    33961 ctatcagaac cattagaggt cgcttcatat aagttattag atgcaatatg tgctaaatcg
    34021 ctattgattt tcaatggttt taatcctttt aattttctca tttcattcgt tacttcataa
    34081 agagaaatta attgatttgg attttgctca actggacgct tattatgctc ttctgacgta
    34141 gaattagaat ttaattgata aggttcaata tctgctaaca tttcttttgt taaaaatcgt
    34201 acacttagca cctttttcga ttgttgatca gaatacactt gtgcatatat gtcgccatat
    34261 ttaatcagtg tttgtgtttt taaatcttca tctgaaagtt caaattcata ttttttacca
    34321 tcaactttaa aggacggttc tggattaata cttgtatgat taaaaatttc tgcagaatgt
    34381 tgtcctattt ttaacggact aacattgact ttctcacctg tagcatacac tgaaacgatt
    34441 tcttcacgtt tagttgaaac aatgtaataa ctgtttttgt ctttaaacac ataatttttg
    34501 taaccatctc taaaagggta gacacgatct gcttgtccaa atttagaagt aagcacttta
    34561 atatctttac ctacccatgt accaacacct tctttaggct ttggattttc agcatggtta
    34621 tttgatttat tcacctgttc agatctattt actttattac ctttattcgg gttctctaat
    34681 acatcaaatt ttaatctcgg tgaataaaaa agatatatta aaaatccaac taaaaataat
    34741 acaccgacga ctcttataat taactttttc atcaatcaac cacctaaaaa gtattaatac
    34801 tattgtaaaa aacaacacat taattagcaa attttcaaca ctgacataac tgtgtcgttt
    34861 cgataaaaca aaatcatctt caggcataat tttaacgtca taaagatttt ccctacactc
    34921 tatatcataa cctatcttta tgttttcagg ttgaatttca ttattaagat taaaatatgt
    34981 ataaaaaaat ggacagttaa ggtatcaaat gaataccatc aactgtccga ctattcttca
    35041 tcaaaaaacc tgataaaaca aattgcctta tcagattagt atcatttgta taagcatatt
    35101 aatgaccaaa tgttgcttta atcagtgatg tcgtttctcc acctggatat aatacatata
    35161 ataataagta aactgctaca cctgtaattg cagtacagaa ccatataact gaagcgaatg
    35221 gcccgaattt gcggtgtaca ttatatttat ctttaaatgc agtaataatt tgaactaggc
    35281 ctagaatacc accaattgtt gctaaattaa tgtggaaaaa taagaaaatc gtataatatt
    35341 tcttaattga tgctggtccg ccaaatgctg tattaccgat aaaaatcgtt ctacttgcat
    35401 agattaagaa aaaagttaaa gcaaaaacag ctgcagctaa cataacattt ttgtgtttat
    35461 ttatttccct tttccaaata agtctccaac caatggcaat taaaattgca ctaatgacaa
    35521 tacatgtcgt actaatcgtt ggtaaaattg gaacgcccat atttttcatc ctaacttaat
    35581 taatctagat caaagtaagt aatgaaacaa tcacagctaa cacgaaaaag atcactaaat
    35641 aatttagtga atatataaac atttgtgttg cccattttgt ttgatctgaa tttttcttaa
    35701 atgttgttaa acctaatgca atccatccta aatttaataa tgtagctaac actacgaata
    35761 cgacacctaa atttattagt aataaaggta ctggcaataa aataatcaac cagataaaca
    35821 tactgacacg tgtacgttta aagcccttaa ctgatggtaa cattggaata tttgcaagtg
    35881 catattcatc tttacgttta atagctaagg cataaaaatg aattggttgc caacaaaata
    35941 caactaaaaa cagcgcaatc gctgttaaac taatttgtcc ttcaattgca acccatccaa
    36001 ttagtggtgg tactgctcca ggaaaactcc caatcactgt gttccatgtt gtatgtcttt
    36061 tagaccatat tgagtaataa gacacataac ctacaatccc cataagacca agtacgcctg
    36121 atggtatatt caataaaaac aaacaaattt ctccaactaa catcatacca aaacttaata
    36181 gtaataaatt ttgatctgta attctattat ttacagttgg tctattttgt ttactaggca
    36241 taatacgatc aatatcttgg tcgtaataat tatttaacgc acatgcgcca cccataatta
    36301 aagtagatcc aaatagcatt aataaaattt gaggtattga tgataagaag gaatgatttg
    36361 tcattacaac tgctagccat gcgcccgcaa aagccggaat taagttacct tgaacaagtc
    36421 ccattttaat tatctgttgc aattctttga agttaactct gctaatattt tgtgacaaag
    36481 tatgctcttt gctcataatc cccctcctta aatttgttca tataagatta tgatatctta
    36541 gattgcataa aaagactagg tttaataaaa ttaaattgtg acaaattaac gacaagagaa
    36601 aatgtcaatt ttgtgacaca aataacattt aatttattgc tataatgtat atgttagaaa
    36661 attttaataa gtagaatcat gcatctaaaa gagattaata tttaagcttc aaatttgagt
    36721 aaacgtggat tacataatta tcccaataaa aaaatcatta cgattaagtt ctttttatgt
    36781 cgtccacata caatacttgt aaaattaaat catatttcct gcgttggatc ccatcttttc
    36841 atatcctaca atcaggtcta tttatagtat catctcaaat ccggctatta attctaattc
    36901 tcagtgatgc gtttttttat gatggggtgt ataaattgtt tggcaaaaag aatttaaaat
    36961 ggttaggtgt cgtagcaacg ttaatgatga catttgtaca acttggtgga gccttagtta
    37021 ccaaaaccgg atcagctgat ggttgtggtt cttcttggcc actatgtcat ggtgcgttga
    37081 ttccagaatt ctttcctatt gatacgatta ttgagttaag tcatagagcc gtttcagctt
    37141 tgtctttatt aatggtctta tggttagtta tcactgcatg gaaacatata ggctatatta
    37201 aagaaattaa acctttatca atcattagtg ttggattctt attattgcaa gcattaatcg
    37261 gagctgctgc tgttatttgg caacaaaacg attacgtttt agcattgcac tttggtatat
    37321 cattaatcag tttctcatct gtatttttaa taacattgat tattttctct atagatcaaa
    37381 aatatgaagc tgacgaatta tatatcaaaa agccattaag acgtttaaca tggttaatgg
    37441 caatcatcat ttattgtggt gtttatactg gtgcactagt gagacatgcg gatgcaagtt
    37501 tagcatatgg tggttggcca ttgccattcc acgatcttgt accacattca gaacaagatt
    37561 gggttcaact cacgcatcgt atcatggctt ttatagtgtt tacgattatt atgattactt
    37621 atattcacgc tgttaaaaat tatccaaata accgtactgt tcattatggt tatacagctg
    37681 catttatact tgttatatta caagttatca caggtgcatt atctattatg acaaatgtta
    37741 acctgataat cgcacttttc catgcattat ttatcactta tttatttggt atgacaacat
    37801 actttatcat gctaatgtta cgatcagtaa gaagtgacaa gcaataacaa aaaagcaaac
    37861 cgtaatttta atggcacgcc cattaaaatt acggtttttt atatcaatat ttaaaattaa
    37921 acctaagcca tgtaaaaacg agattacacg tcaattgttg tgtaatctcg ttttatttta
    37981 atcattttag tcagttgctt tttcaatttc gattaataaa tcgcctgtcg ctattgtgtc
    38041 accattattt acagttactt gtttaatcac accgtcaaat ggtgcttgaa ttgttgtttc
    38101 cattttcata gcttcagtaa ttagcaacgg ctgattagct ttcacagttt cacctacact
    38161 aaccttgact tcagttactg aacctggcat ttgagcaccg atatgacttg gattactctt
    38221 atctgctttt ggcttaacgt tcgcatttgt atgcacattt tcatctttaa tgtaaatacg
    38281 tctcgcttga ccattcatcg catagtaaat cgttctatta ccattttcat ctggttcact
    38341 aatcgtttct agtttaataa ttaatcgttt acctttatcg atttcgattt ctactgtttc
    38401 accattacgc attccaaaga agaatgtagg cgtatcaagt aacgataagt ttccgtattg
    38461 atttctagtt tgaatatatt gttcatatac ttttggatat agtacataac taataatatc
    38521 ttgctccgta acaggacctt gttgctcttc ttcaagcaac tcacggactt tttcaaaatc
    38581 aactggctct agatattcac ctggacgagc tgttagtgct tcttggcctt ttaaaataac
    38641 cgcttgtaaa tctttattaa aaccatttac aggttgtcct atttcacctt tgaagaacga
    38701 cactactgat tctgggaaat ctaatttata gccatctgta atcacggatt gttcatcaag
    38761 atcattttgt accatataaa gtgccatatc accaactact ttagacgatg gtgttacttt
    38821 tacgatatca ccaaataaga aattcactct gcgatacata tctttgactt catcaaatct
    38881 ttcgcctaaa cctaaacttt tagcttgttg acttaaattc gaatactgtc caccaggcat
    38941 ttcatgttga taaatttcag tattcggtga tttgatatca ctttcaaagt ctgaataata
    39001 agtacgtaca gttgaccaat aatgactaag tgactccata ccttcaatat cagttctaag
    39061 gtggcgtggg aagccattta atgcataata taacgaattg gcgcttggct gacttgttaa
    39121 accactcatt gaagcaacag cagtatcaat gatatcgaca ccagcatcta ttgcttgttt
    39181 gtatgttaat aaaccattac cacttgtatc atgagtgtga agatgaattg gtaaatctac
    39241 agctgatttt aactcaccaa tcaattcgta agcggcttta ggttttaata agcctgccat
    39301 atctttaatc gctaaaatat ggaaaccttc acgttctaac tctttagcta gtttgacata
    39361 atactctaaa gtataaatgt ttgatcgctc aggatttaaa atgtcacctg tataacaaat
    39421 agtaccttct gagattttgc ccgcttcttg tactgcttca ttggcaactt tcatttgatc
    39481 tacccagttt aatgaatcga aaattctaaa gacatctatg cctgctttag cactttcttg
    39541 tacgaattta tgaataacat tatcaggata gtttttataa ccaactgcgt ttgaagcacg
    39601 taacaacatt tggaataata catttggaat agctttacgt agacgttcaa gtcgttccca
    39661 tgggttttcc ttcaagaaat tatatgccac atcaaatgta gcaccgcccc acatttctag
    39721 tgagaaacca tctttaaata cgtccgctgt tttggatgcg atattaatca tatccttagt
    39781 tctaactcgt gtagctaata atgattggtg tgcatctcta aaggttgtat ctgttagtaa
    39841 gacatcatcc tgctttttaa cccattcagc tacacctttt ggacctactt catcaagcaa
    39901 ttgtttcgta ccactaaatg aagcgatttt acttgaagat acagttggaa ttgatgctaa
    39961 ttcatagtct ggtttcggac gtttctcaac atttgggaaa ccattaattg ttacattacc
    40021 tatatattct aatgttttag tacctctatc tagagacggc tgaatgttga aaagttctgg
    40081 tgtttcttca ataaatttag ttgtgtaatc accacttgtg aactttttat tcttcattac
    40141 attaattaag aatggaatat tagttttaac accacgaata cgcatttctc gtaatgagcg
    40201 taccattttt tcttctgctt gtttaaatga tatcgcgtgt gtagataatt ttacgagtaa
    40261 tgaatcataa taaggtgata tctcagcacc ttggaaacca tctccagcat caagacgtac
    40321 accaaagccc ccgcttgaac gataagcaat gattgttcca gtatccggca tgaaatcatt
    40381 taacggatct tctgttgtaa tacgacattg gatggcatag cctaatgttg taatatcttt
    40441 ttgttgcggc atattaatct cttcaccaaa taaatcggca cctgctgcaa ctaaaatttg
    40501 tgtcttaaca atatcaattc ctgttaccat ctctgtaatt gtatgctcta cttgtacacg
    40561 agggttaact tctataaaga agaattcgtc accagatact agaaattcaa cagtacctgc
    40621 attgacatat ttaatatttt ccatcaattg aattgcagca tcacaaatac gttgacgtaa
    40681 tgttgatgat aatccaactg atggtgcaac ttctacaact ttttgatgac gacgttgtac
    40741 tgaacaatca cgttcaaata agtgtacgat atttccatgt tcgtcaccta tgacttgtac
    40801 ttcaatatgc tttggattat caatgtatct ttctatgtaa acttcactat taccaaatga
    40861 tttttcagct tctgattttg ctctatggaa agcatcttct aattcacttt cttcacgaac
    40921 gattctcata cctttaccgc cgccaccact tgtggcttta atcattagcg ggaaaccagc
    40981 ttcttctgca aattcttttg ctaattcata tgatttaatt ggaccgtctg taccaggaat
    41041 aactggtaaa tctgccttga tagccgttgt acgagcttta actttatctc caaacatatc
    41101 taaatgttct aaatgaggac caataaattt aattccttct tctgcacaac gacgcgcaaa
    41161 ttgttcattt tcacttaaaa atccatagcc aggatgaatc gcatccacat tcgcttgttt
    41221 tgctacatca atgatacgct caatatttaa ataactttca gcaggaccta aatcacttcc
    41281 aactaaatag gattcatctg ctttatatct atgtaatgaa cttttgtctt cattcgaata
    41341 aattgcaact gtgctgatgt ctaattctgc cgccgctctg aatatacgaa ttgcaatttc
    41401 tccacggtta gcaacaagta acttttttat ttgtttcaat agcgatacac tcctcaaact
    41461 attagaattt tctaactaat tagataataa aattttatct taaagcgctc tgttttgcta
    41521 tagttatgtt tcaaattttc aaaatttaac attcttgaga caattaaaac ctccgcttca
    41581 gaaataataa tttcaaaaat gactatgcaa caacaggtag ttcacgtttt tgttgtgaaa
    41641 cattttcgat ttctacaact ctaaaaaatt aaaaataaaa ttgcaaaaca tcaacattta
    41701 ttatcaatag cgataacttt atcttatcat catgattcta atttcgccac cacatttagt
    41761 aatttttag
//
LOCUS       DALAGD010000005        40670 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.005, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000005 DALAGD010000000
VERSION     DALAGD010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 40670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40670
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.005"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(134..1771)
                     /locus_tag="O2701_000203"
     CDS             complement(134..1771)
                     /locus_tag="O2701_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCZ4292119.1"
                     /translation="MNKDKQLHNDKINLSQLVLLGLGSLIGSGWLFGAWEASSIAGPA
                     AIISWVLGFLVIGTIAYNYIEIGTMFPQSGGMSNYAQYTHGSLLGFIAAWANWVSLVT
                     IIPIEAVSAVQYMSSWPWHWAKPMRYLMENGSISTYGLLAVYLIIVIFSLLNYWSVKL
                     LTSFTSLISVFKLGVPMLTIIMLMLSGFDTSNYGHSASTFMPYGSAPIFAATTASGII
                     FSFNSFQTIINMGSEIKNPEKNIARGIAISLSISAVLYIILQSTFITSMPQSMLQHSG
                     WNGINFNSPFADLAILLGINWLAILLYIEAFVSPFGTGVSFVAVTGRVLRAMEKNGHI
                     PKFLGKMNEKYHIPRVAIIFNAIISMIMVTLFRDWGTLAAVISTATLVAYLTGPTTVI
                     ALRKMGPTMTRPFRAKILKVMAPLSFVLASLAIYWAMWPTTAEVILIIILGLPIYFFY
                     EYRMNWRNTKKQIGGSLWIIVYLIVLSILSFIGSKEFKGLNMIHYPFDFIVIIIVALI
                     FYYIGTTSSFESVYFRRATRINTKMRESLNNESKSSH"
     gene            1951..2550
                     /locus_tag="O2701_000204"
     CDS             1951..2550
                     /locus_tag="O2701_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family protein"
                     /protein_id="HCZ4292120.1"
                     /translation="MSEKQSNEQVEAFLNKESQWQEEYKYLRALIFNETELEEAYKWM
                     HPCYTLNNKNVVLIHGFKNYVALLFHKGAILEDKYHTLIQQTEKVQAARQLRFENLTE
                     IQARTEEIKYYLAEAIKAEKAGKKVEMKKTEEYVIPKELEAKFEEMPQLESSFYKLTP
                     GRQHQYIYHIGQAKRSETRQKRVEKYINQILEGKGMHDK"
     gene            2554..2694
                     /locus_tag="O2701_000205"
     CDS             2554..2694
                     /locus_tag="O2701_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292121.1"
                     /translation="MSKAYRLYNNIQDDTKQPMAHAVGCFFRCVCHGQHFDVGIPLQA
                     WE"
     gene            2774..2893
                     /locus_tag="O2701_000206"
     CDS             2774..2893
                     /locus_tag="O2701_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292122.1"
                     /translation="MGGCPSVTMDACALLQKFDFCNNISHFRHFFAIKQPIER"
     gene            3220..4446
                     /locus_tag="O2701_000207"
     CDS             3220..4446
                     /locus_tag="O2701_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4292123.1"
                     /translation="MLSIKHLTKIYSGNKKAVDDISLDIQSGEFIAFIGTSGSGKTTA
                     LRMINRMIEATEGQIEIDGKDVRSMNPVELRRNIGYVIQQIGLMPHMTIKENIVLVPK
                     LLKWTKEEKDKRAKELIKLVDLPESFLERYPAEISGGQQQRIGVVRALAAEQDIILMD
                     EPFGALDPITRDTLQDLVKTLQRKLGKTFIFVTHDMDEAIKLADKICIMSEGKVVQFD
                     TPDNILRHPANDFVRDFIGQNRLIQDRPNDKTVEGVMIKPITIQAEATLNDAVHIMRQ
                     KRVDAIFVVDSNNHLLGFLDIEDINQGIRGHKSLRDTMQQHIYTVQIDSKLQDSVRTI
                     LKRNVRNVPVVDDQQRLVGLITRANVVDIVYDTIWGDSEDTVQTEHVGEDTASSKVHE
                     QHTTNVKVRDIGDDKS"
     gene            4443..5078
                     /locus_tag="O2701_000208"
     CDS             4443..5078
                     /locus_tag="O2701_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4292124.1"
                     /translation="MIEFLHEHGGQLMSKTLEHFYISIVALLLAIIVAVPIGILLSKT
                     KRTANIVLTVAGVLQTIPTLAVLAIMIPIFGVGKTPAIVALFIYVLLPILNNTVLGVQ
                     NIDSNIKEAGKSMGMTQFQLMKDVELPLALPLIIGGIRLSSVYVISWATLASYVGAGG
                     LGDFIFNGLNLYDPLMIVTATVLVTALALGVDALLALVEKWVVPKGLKVSG"
     gene            5095..6036
                     /locus_tag="O2701_000209"
     CDS             5095..6036
                     /locus_tag="O2701_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HCZ4292125.1"
                     /translation="MKKIKYILVVFVLSLTVLSGCSLPGLGSKSTKNDVKITALSTSE
                     SQIISHMLRLLIEHDTHGKIKPTLVNNLGSSTIQHNALINGDANISGVRYNGTDLTGA
                     LKEAPIKNPKKAMIATQQGFKKKFDQTFFDSYGFANTYAFMVTKETAKKYHLETVSDL
                     AKHSKDLRLGMDSSWMNRKGDGYEGFKKEYGFDFGTVRPMQIGLVYDALNSEKLDVAL
                     GYSTDGRIAAYDLKVLKDDKQFFPPYAASAVATNELLRQHPELKTTINKLTGKISTSE
                     MQRLNYEADGKGKEPAVVAEEFLKKHHYFDKQKGGHK"
     gene            6036..6731
                     /locus_tag="O2701_000210"
     CDS             6036..6731
                     /locus_tag="O2701_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4292126.1"
                     /translation="MEGNLLQQLFNYYVTNFGYLWDLFFKHLLMSVYGVLFAALIGIP
                     LGILLARYTKLSGFVITIANIIQTVPVIAMLAILMLVMGLGSETVVLTVFLYALLPII
                     KNTYTGIASVDANIKDAGKGMGMTRNQVLRMIELPLSVSVIIGGIRIALVVAIGVVAV
                     GSFIGAPTLGDIVIRGTNATDGTTFILAGAIPIAIIAIVIDVLLRFLEKRLDPTTRHR
                     KNQSNHRPQSINM"
     gene            7025..8425
                     /locus_tag="O2701_000211"
     CDS             7025..8425
                     /locus_tag="O2701_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ4292127.1"
                     /translation="MDTSKQFRGDNRLLLGIVLGVITFWLFAQSLVNLVVPLQSTYSS
                     DVGTINIAVSLSALFAGLFIVGAGDVADKFGRVKITYVGLILNVVGSLLIIITPLPAF
                     LIIGRIIQGLSAACIMPSTLAIINEYYIGTRRQRALSYWSIGSWGGSGICTLFGGLMA
                     TYIGWRSIFVVSILLTLLAMYLIKHAPETKAEPIKGMKAEAKKFDVIGLVILVVTMLS
                     LNVIITQTSHFGLVSPLILGLIVVFIYSLVGFVYYENKIKHPLVDFSIFKNRGYSGAT
                     ISNFLLNGVAGGALIVINTYYQQQLGFNSSQTGYISLTYLITVLSMIRVGEKILSQHG
                     PKRPLLLGSGFTVIGLILLSLTFLPEVWYIISSIVGYLLFGTGLGLYATPSTDTAVAS
                     APDDKSGVASGVYKMASSLGNAFGVAVSGTVYTVLAANLNLNLGGFTGMMFNALLAIV
                     AFLVILLLVPKNQTNL"
     gene            8651..9586
                     /locus_tag="O2701_000212"
     CDS             8651..9586
                     /locus_tag="O2701_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCZ4292128.1"
                     /translation="MKIAIAGSGALGSGFGAKLFQAGYDVTLIDGYTSHVEAVKQHGL
                     NITINGEAFELNIPMYHFNDQPDESIYDVVFLFPKSMQLKEVMEDMKPHIDNETIVVC
                     TMNGLKHEEVIAQYVAQSQIVRGVTTWTAGLESPGHSHLLGSGPVEIGELVDEGKENV
                     IKVADLLNEAELNGVISKDLYQSIWKKICVNGTANALSTVLECNMASLNESSYAKCLI
                     YKLTQEIVHVATIDNVHLNVDEVFEYLVDLNEKVGAHYPSMYQDLIVNNRKTEIDYIN
                     GAVATLGKQRHIEAPVNRFITDLIHTKESQRHAQD"
     gene            9908..10759
                     /locus_tag="O2701_000213"
     CDS             9908..10759
                     /locus_tag="O2701_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCZ4292129.1"
                     /translation="MSKIFVTGATGLIGIKLVQRLKEEGHEVAGFTTSENGQQKLAAV
                     NVKAYIGDILKADTIDQALADFKPEIIINQITDLKNVDMAANTKVRIEGSKNLIDAAK
                     KHDVKKVIAQSIAFMYEPGEGLANEETSLDFNSTGDRKVTVDGVVGLEEETARMDEYV
                     VLRFGWLYGPGTWYGKDGMIYNQFMDGQVTLSDGVTSFVHLDDAVETSIQAIHFENGI
                     YNVADDAPVKGSEFAEWYKEQLGVEPNIDIQPAQPFERGVSNEKFKAQGGTLIYQTWK
                     DGMNPIK"
     gene            complement(10851..12347)
                     /locus_tag="O2701_000214"
     CDS             complement(10851..12347)
                     /locus_tag="O2701_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCZ4292130.1"
                     /translation="MTTKKLYFLSISIIILVAISIAIYITLNSNTKTRLTNDSQQQID
                     TIIEHDLQKGHIPGASILIVKNGKVFLNKGYGYQDVDKKVKASPTTKYEIASNTKAFT
                     GLAILKLAQEGRLNLNDAVSKHVPHFKMNYNGQNETITIKQLLAQTSGIPSDITSEDS
                     VTSKNNRLNDVTRAIMGDELHHKPGEEFEYSNMNYDLLGLIIQNVTKQSYTKYITNSW
                     LKPLHMTHTSFKQTNYKSKHDAIGYELQGSTPVVSKPEFNLWDTPSAYMMTSTEDLEH
                     WIKFQLNPPDKYKSLVQQSHKNLSSTIGEPNANAYASGWFTNNDEHLVFHSGTLDNFS
                     SFILLNPKQNYGIVVLANLNSEYVPKLVEHLNTQIVNHKRYSTVASMLNQYKDQFNIV
                     TVLMTTLILLAFIFSAYRAWQMRHGQILLRRSKRIAVLSWLSLCICIALALILYALPY
                     LILGSNNWSFVLTWLPIEIKLALITTLIALFSTLIVILLFLHTKITKT"
     gene            12579..14408
                     /locus_tag="O2701_000215"
     CDS             12579..14408
                     /locus_tag="O2701_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCZ4292131.1"
                     /translation="MFNQFKRLIIGQPKKNRELKDEKISKFKGLAILSSDALSSVAYG
                     PEQILITLSVVGAVATWYTLPIAGAVLILLAALIMSYRQIIYAYPKGGGAYMVSKTNL
                     GEKWGLLAGGSLLVDYILTVAVSISSGADAFVAAFPSLYGHKVLIACLLVLFILILNL
                     RGLTESATVLSYPVYLFIIGLVILIFIGTFRVATGDIQPHMHASVGTAVPGVTLFLLL
                     KAFSSGASSLTGVEAISNAVTNFREPSAKNAVKTLIAMGSILAFLLVGIVGLAYVYGI
                     LPQTETTVLSQLAMQIFGDNAAFYFVQATTVMILVLAANTGFTAFPMLAASMSKDKYM
                     PRMFTVRGDRLGYSNSIIILGVLAIILIIVFDGMTEDLIPLYAVGVFIPFTLAQFGMV
                     IKWIHERPKNWLSKLSVNLLGGIVTFIVFMILLITKFSQVWPILIFLPFVVIFFLKIN
                     KHYRDIAEQLRSDIDVLNVDVVDRNLAIVPITSITTAVDKSIYYAQMLANNDVIAVHV
                     SFGDEDEKAFQEKWKRHFPDVRLVILHSEYRSIIRPISRFIDKINRKANDQNYMITVV
                     IPEFITKKRWHNLLHNQTSLRMKLYLIYQKNVNVCTIPFKLKK"
     gene            complement(14548..16626)
                     /locus_tag="O2701_000216"
     CDS             complement(14548..16626)
                     /locus_tag="O2701_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCZ4292132.1"
                     /translation="MALLEAFLIFIFAVIISSVINNRFPQIPTAFIQIALGVVIFIIP
                     IQVDFQFNSEVFMFAVIAPLLFVEGTHVSRTKLLEYRKPILLMSMALVFATVVGVGYF
                     IHWIWPALPMPAAFAIAAILCPTDAVAVSAITRGKLLPKGSMTILEGESLLNDAAGII
                     SFKIAVTALVTGTFSLFQAVEQFIISTILGVLIGAIIGFVVVRIRIDLTANKGLKDNN
                     TLTFIQLLTPFVVYFLAEEVHASGIIAVVIAGLIHGLERDRLIRAQTELQMNYHQIWN
                     TFSYALNGFVFVVLGFMIPTVVIDIFQTEPDNFSFLIVITILIAIAIYACRFVWVYFW
                     YKDFYFPKNIQSYLDEEHDSHETPPSRVRYAFIMTMCGIHGTISLSMALTLPFIITKG
                     QAFEYRNDLLFIASFMVLISLILAQIVLPLITPSAEDTTFKGMTYQSAKIFIVQKVIQ
                     HFKNESKKDKNDTNYRPVLNQYYGELLFLLNSEPDNQNTKELKRLEDIAKVIETSTLE
                     RLIDKGKATYQDINNYRNIVELTETHRTASFVEKFANFFKMLWLRLKAMKHYKALNKE
                     SKKQEFENSFKDVQKIMRIVNHNIILRLKEEQNSTNVLEVSLVINHYYDMSRSLKWRA
                     QRRKERQENSNQIIPQAMFHNHKLEALYLQRHLLDELIRKNKINNIVAAQIRENINYN
                     EIVLSLQSKH"
     gene            complement(16764..18173)
                     /locus_tag="O2701_000217"
     CDS             complement(16764..18173)
                     /locus_tag="O2701_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCZ4292133.1"
                     /translation="MARKLHRELNNRHIQLIAIGGAIGTGLFLGSGQTISLTGPSLLF
                     TYMIIGVVLFAFMRALGELLLSNTRFNSFVDIANEYLGPFGGFVIGWTYWLCWIVSSM
                     SDLTAMGQYFAFWYPQVPNWITVLFIVLILISFNLLGARLFGELEFWFSIIKVVTIIA
                     MVIVGLVLIFFSFKTHYGHASFTNLISHGGMFPGGTFGFLMSFQIAVYSFIGIELIGV
                     TAGETKDPEKTLPKAINNVPIRILLFYIGGLLVIMSVIPWNDIDPNSSPFVKLFTLIG
                     VPFAAGVVNFVVLTAAASATNSGIYSNSRILFGLSQQGLGPKVLNKTNSHGVPYLSML
                     VSSIALLIAALLNYIFPNAIQLFIYVTTLSTVLFLVVWAMIIVAYLMYLKKHPEAHKN
                     SKFKLIGGKPIAYIILAFFFFVFILLFFSDETRAAIYISPFWFIFLFFFYKKYKTNAE
                     KLAYEQRQNDSGHFRYDNQ"
     gene            18459..19265
                     /locus_tag="O2701_000218"
     CDS             18459..19265
                     /locus_tag="O2701_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCZ4292134.1"
                     /translation="MKIGTIADLHIDRHNKKTSEDYLEALVEIVKYKKLDILLIAGDI
                     SNHYQLTHQFITQLTKQLDIPVKFVPGNHDLWEVESMTTQDIWNNYKSMSQCLVGKPF
                     IVNEEWAIIGHTGWYDYSFAAQRFSLDELQKGKHYGATWQDKERISWGISDQNLSKIA
                     AEQVKKDILEVGNRRVILVTHVVTHPDFIVPMPHRIFDFYNAFIGTSDFNPLYAMFDI
                     PYSIMGHVHFRKSVIDDGRCYLCPCLGYPRQWRSEDIYQEINETIQIIEI"
     gene            complement(19328..19984)
                     /locus_tag="O2701_000219"
     CDS             complement(19328..19984)
                     /locus_tag="O2701_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HCZ4292135.1"
                     /translation="MNRKILPVKPISQLFPIPMVMGCEGVSAAMMLQYNNQHIPATEI
                     MRHWPTHPNNPHKGYVGHHLFIKLGNYHQTIFPEAYVPFLQKYNPNIVDGTGKSLEDL
                     KGIIDQGQPVLIYHTNLGSKPLLRVFRFDNKPAKQVSNIHVTLLIGYDDYYYYYIDPL
                     WSHIRRGLVLPAIIPNRKQIIKIRKEKMEYSFNSPGRKCIYVQPHSYTIENQQQNKHT
                     "
     gene            20097..20570
                     /locus_tag="O2701_000220"
     CDS             20097..20570
                     /locus_tag="O2701_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCZ4292136.1"
                     /translation="MEYQLQQLASLTLVGIKETYENGRQAQQHIAGFWQRCYQEGVIA
                     DLQLKNNGDLAGILGLCIPELDGKMSYMIAVTGDNSADIEKYDVITLASSKYMVFEAQ
                     GAVPKAVQQKMEEVHHYIHQYQADTVKSAPFFELYQDGDTTSEKYITEIWMPVKG"
     gene            complement(20693..21904)
                     /locus_tag="O2701_000221"
     CDS             complement(20693..21904)
                     /locus_tag="O2701_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HCZ4292137.1"
                     /translation="MQSTKTKTKHFSFLLLITLGVMTAFGPLTIDMYVPSLPKVQGDF
                     GSTTSEIQLTLSFTMIGLALGQFIFGPLSDAFGRKRIAVSILIIFILVSGLSMFVDQL
                     PLFLTLRFIQGLTGGGVIVIAKASAGDKFSGNALAKFLASLMVVNGIITILAPLAGGL
                     ALSVATWRSIFTILTIVALIILIGVASQLPKTSKDELKQVNFSSVIKDFGSLLKKPAF
                     IIPMLLQGLTYVMLFSYSSASPFITQKLYNMTPQQFSIMFAVNGVGLIIVSQVVALLV
                     EKLHRHILLIILTIIQVVGVALIILTLTFHLPLWVLLIAFFLNVCPVTSIGPLGFTMA
                     MEERTGGSGNASSLLGLFQFILGGAVAPLVGLKGEFNTSPYMIIIFITAILLVSLQII
                     YFKMIKKQHVA"
     gene            complement(22086..22745)
                     /locus_tag="O2701_000222"
     CDS             complement(22086..22745)
                     /locus_tag="O2701_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292138.1"
                     /translation="MKRTDKYRDSYQYDNQNQNHRRQSEDASYRQQYAKGDPEEHPER
                     YYNGRDYRREQILEEENEKSRRSKKWLYIIIAILLIIVAIFVTRALLNNDSDKVSNDP
                     KVSQNYKKQVENQDGQINQQVDNAKENIKNNQKTDDIIKNLQNQIDNLKQQEQNKADS
                     KLTQFYQDQINKLTEANNALKNNASQGKIESMLNDINTKFDSIKSKLESLFKDDNGGA
                     N"
     gene            complement(22805..23947)
                     /locus_tag="O2701_000223"
     CDS             complement(22805..23947)
                     /locus_tag="O2701_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCZ4292139.1"
                     /translation="MQLQKIVIAPDSFKESMTAQQVGNIIKQAFTNVYGNTLHYDIIP
                     MADGGEGTTDALMHATGATKYTVIVNDPLMRPIEACYARADEQQIAIIEMAAASGLDL
                     LEKEERNPLYTSSYGTGELIKDALNHGAKTIILGIGGSATNDGGTGMLSALGVKFTDV
                     NGDLLQMNGANLAHIAQIDITNLDSRLKEVTFKVACDVSNPLLGENGATYIYGPQKGA
                     DAKMIPKLDFAMSHYHDKIKMCTGKSVNQIPGSGAAGGMGAALLAFCETTLTKGIDVV
                     FDITDFHQRIKDADLVITGEGRMDYQTIFGKTPVGVALAAKQYHIPVIAICGSLGENY
                     QHVYDFGIDSAYSIISSPSTLEDVLQNSEQNLLNTATDIARILKLQ"
     gene            24215..24601
                     /gene="gtxA"
                     /locus_tag="O2701_000224"
     CDS             24215..24601
                     /gene="gtxA"
                     /locus_tag="O2701_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase GtxA"
                     /protein_id="HCZ4292140.1"
                     /translation="MKLTQTHAEILKFIIVGGINTLNYYVVYLLLLKLLHIEYMISHI
                     TGFLVAFVISYYLNCYFVYRVKPTWRKFISFPITQIVNVSLQTVLLYVFVSWLNLPAE
                     IAPFAGLIITIPITFILSKWILKDSN"
     gene            24735..24842
                     /locus_tag="O2701_000225"
     CDS             24735..24842
                     /locus_tag="O2701_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCZ4292141.1"
                     /translation="MFNLLINIMTSAISGCLVAFFAHWLRTRNNKKGDK"
     gene            25470..27233
                     /locus_tag="O2701_000226"
     CDS             25470..27233
                     /locus_tag="O2701_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4292142.1"
                     /translation="MNNQTNWIKILSGFASDSKWKIMLSILLSIISVFSGLVPYWAVF
                     KIILMMINNTYTINSIMVYIFIALIAYISQVCCFGASTMLSHITAYEILSEIRKKLAQ
                     KLMRLPLGVVESKKIGELKNIFVDKVETIELPLAHMIPEVIGNLLLSAAIFLYIMLID
                     WRMASALLVTIPISIFAFKKVMSGFNETYAEQMKSNNYMNSAIVEFIEGIEVIKTFNQ
                     SQSSYKKYKDAVDNYKIHTLNWFKNTWGYMNLGASVLPSTFLGILPVGMYLISINQLN
                     YAEFFLCIVLSLGVVAPIKNFTNYVNHLKSIQYALTEVNQILSLEELVLSTKFKKPQH
                     YEIAFNNVGFSYNKDKDDLVFKHLSFTVPENNFTAIVGASGSGKSTIAKLISRYWDVT
                     SGEITIGGINIKDIESKQLNDLVGFVGQDNFLLNLTFKENIKLGNPEATDEAVEKAAK
                     LAQCHEFIEKLPDGYDTNVGTVGDKLSGGEKQRVTIARMILKDAPIIVLDEATVYVDP
                     DNEQKIQEALNVLTQDKTLIVIAHRLSTIQHADQIIVLGKQQILEKGSHHLLLKLNGN
                     YKKMWDTHMHTKDWGINTGHN"
     gene            27258..28991
                     /locus_tag="O2701_000227"
     CDS             27258..28991
                     /locus_tag="O2701_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4292143.1"
                     /translation="MFQITFKILNWIRPYKARMILGFSMSFLNAIFIALPIFLAAKIF
                     NNVLSHKPIYMKDILNVVIIMVLLVIGRFITAYFKSKSHESIAYEMSAKERLDIGDKL
                     KNVRLGYFNSHHSNELTTIVTTDLTFLENFAMKMVDVVVNGYILITVLILSLLVVSWQ
                     VSLLACIGVLLSFFAIQLLERKSRQNAPAYHNVQNQLVEKVLEVIRGIQVIKSFAKEN
                     TSLKSFNQAVNESKRINTKIEMQYIPFNLLHLLSLKVVSIMIVLVACLLYMNHSIDLP
                     TLIMISIFSFVIFDSVENINSAAHVLEMIDMTIDDIEKIKNAPELDENGKNLTIKNEN
                     IAFQNVNFSYDDKQVIKNVNFEIPTQTSTAIIGPSGSGKSTLCHLLLRFYDIDDGNIR
                     IDGVDIKDMTLSTLMSKISAVFQKVYLFNDTIENNILFGNPGATKEEIIRAAKQACCH
                     DFIMSLPEGYQTMLNEKGSNLSGGEKQRISIARAILKDAPIIILDEATASIDPENEQL
                     IQTAINELSKGKTVITIAHKLETIKNADQIIVLNEGEIIQKGSHDELIRKPGMYQDFI
                     RIKSKSAGWKL"
     gene            29222..29389
                     /locus_tag="O2701_000228"
     CDS             29222..29389
                     /locus_tag="O2701_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292144.1"
                     /translation="MQLLILTDIFYRIAVYICPNSLRSLSHFNSKVKILPKFIVILRS
                     LNSCFLEKHAP"
     gene            29443..30129
                     /gene="bioD"
                     /locus_tag="O2701_000229"
     CDS             29443..30129
                     /gene="bioD"
                     /locus_tag="O2701_000229"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HCZ4292145.1"
                     /translation="MRIFITSTNTDVGKTYVTKHLYHALKTRGHRVCIFKPFQTEERQ
                     DGTFPDLEVFKNECDLSYDITSLYTFKQPVSPHLAFKMTDQIFLNKQRVLDKVKVLDK
                     EFDFILIEGAGGIAVPIYEGTDDFYMTKDLINDCADCVISVLPSKLGAISDAIVHQDY
                     VNQNVSASNFLIMNRYTDSYIEKDNQMTIGKLTNKTVYTFEEHATYENFSEAFLKQLI
                     GVKNELHTTT"
     gene            30107..31465
                     /gene="bioA"
                     /locus_tag="O2701_000230"
     CDS             30107..31465
                     /gene="bioA"
                     /locus_tag="O2701_000230"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCZ4292146.1"
                     /translation="MNYTQQLKQKDSEYVWHPFTQMGVYSKEEAIIIEKGKGSYLYDT
                     NGNKYLDGYASLWVNVHGHNNKYLNKVIKKQLNKIAHSTLLGSSNIPSIELAEKLIEI
                     TPSNLRKVFYSDTGSASVEIAIKMAYQYWKNIDREKYAKKNKFITLNHGYHGDTIGAV
                     SVGGIKTFHKIFKDLIFENIQVESPSFYRSNYDTENEMMTAILTNIEQILIERNDEIA
                     GFILEPLIQGATGLFVHPKGFLKEVEKLCKKYDVLLICDEVAVGFGRTGKMFACNHED
                     VQPDIMCLGKAITGGYLPLAATLTSKKIYNAFLSDSHGVNTFFHGHTYTGNQIVCTVA
                     LENIRLYEKRKLLSHIETTSSTLEKQLHALKRHRNVGDVRGRGLMFGVELVTDKDSKT
                     PLEIEKVERIVRNCKENGLMIRNLENVITFVPVLSMSNKEVKTMVRIFKKAVHNILDR
                     KC"
     gene            31467..32474
                     /gene="bioB"
                     /locus_tag="O2701_000231"
     CDS             31467..32474
                     /gene="bioB"
                     /locus_tag="O2701_000231"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCZ4292147.1"
                     /translation="MNLAKRILQGEQLTKETVLKIYEDTNIDTLDLLNEAYILRKHYF
                     GKKVKLNMILNAKSGICPENCGYCGQSRDIKQKQRYALIPEEQIIDGAKVAHDNHIGT
                     YCIVMSGRGPSDKEVDHISNTVRTIKSQHPQLKICACLGLTNDEQAKKLKSAGVDRYN
                     HNINTSENYHDNVVTTHSYKDRTDTIELMKANNISPCSGVICGMGESNQDIVDMAFAL
                     KEMDADSIPINFLHPIKGTKFGSMDDLTPMKCLRIVALFRLINPTKEIRIAGGREVNL
                     RSLQPLALKAANSIFVGDYLITGGQPNQLDYDMINDLGFEIDYDTCENKENKNEVSRA
                     N"
     gene            32452..33567
                     /locus_tag="O2701_000232"
     CDS             32452..33567
                     /locus_tag="O2701_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HCZ4292148.1"
                     /translation="MKFQEQIDLLQQKGLYRSLKSVGYIDRQYIEVENKRCTNYTSND
                     YLGLGQIAFDKDDFERFMRKYSYHLSSSRLISGSSTAYEEIETMLAGWLGYSACTILN
                     SGYDANLALFNIFKNTNCVVFSDQENHASIIDGIKLSGLEKVIYKHLDIADLEKRLEK
                     YPNQNIPKIIISDSVFSTNGDVVDIGQLVSLKHKYNATLILDVSHSFGIENYSNYQGV
                     DILTSSLSKACGAYGGVILSSNDVKDMLINHGRPLIYSSSLPIYNLYFIKRNIEKLIN
                     ADDRRTKLNSLSKYFNQKLKALNVNYNSSNSPIKFIEFDDIEAAENIHQTLLKHHVFT
                     SYLRYPTVTKPMLRISLSYFHTEQDIDRLFEILHQED"
     gene            33578..34270
                     /locus_tag="O2701_000233"
     CDS             33578..34270
                     /locus_tag="O2701_000233"
                     /EC_number="6.2.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxyhexanoate--CoA ligase"
                     /protein_id="HCZ4292149.1"
                     /translation="MYSIKMRSSNQDVHISGAETICEFDKIEQTVQRFYNKGFFHENG
                     QPDFLNIKIQKIMEPIQQIKALQIIEDDKANLQHLTQECGVTEQALNQGMTYIKNETV
                     YTGAIILSAISGKRLDSFGQRGIRATHFSFEDINNKGDLNERVTDALAIASCINAHPY
                     VKGELCVSDDLTYTTGYFAAAKIGYHRLFDIKPVNTRYGGRIIFVDDCIDLNHYISFL
                     ESTPKQVVYETV"
     gene            34281..34748
                     /locus_tag="O2701_000234"
     CDS             34281..34748
                     /locus_tag="O2701_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCZ4292150.1"
                     /translation="MTSKDITQISVIAAILTILAVLKIPSIIPGLDFQLSAPAALLIL
                     AFFGIKKYFLGGLLSSLLLLVFGVFNPINVIISIIFRVIAIAVVYLLKINVLSLVLAS
                     VLGSLVSRLLLSIILNLPVWVVLLNAIPGVIFTLIVAIPLYLTLRKRMAVLLR"
     gene            complement(34800..35777)
                     /gene="hlgB"
                     /locus_tag="O2701_000235"
     CDS             complement(34800..35777)
                     /gene="hlgB"
                     /locus_tag="O2701_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB/HlgCB subunit
                     B"
                     /protein_id="HCZ4292151.1"
                     /translation="MKMNKLVKSSVATSMALLLLSGTANAEGKITPVSVKKVDDKVTL
                     YKTTATADSDKFKISQILTFNFIKDKSYDKDTLVLKATGNINSGFVKPNPNDYDFSKL
                     YWGAKYNVSISSQSNDSVNVVDYAPKNQNEEFQVQNTLGYTFGGDISISNGLSGGLNG
                     NTAFSETINYKQESYRTTLSRNTNYKNVGWGVEAHKIMNNGWGPYGRDSFHPTYGNEL
                     FLAGRQSSAYAGQNFIAQHQMPLLSRSNFNPEFLSVLSHRQDGAKKSKITVTYQREMD
                     LYQIRWNGFYWAGANYKNFKTRTFKSTYEIDWENHKVKLLDTKETENNK"
     gene            complement(35779..36726)
                     /gene="hlgC"
                     /locus_tag="O2701_000236"
     CDS             complement(35779..36726)
                     /gene="hlgC"
                     /locus_tag="O2701_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgCB subunit C"
                     /protein_id="HCZ4292152.1"
                     /translation="MFKNKILTTTLSVSLLAPLANPLLENAKAANDTEDIGKGSDIEI
                     IKRTEDKTSNKWGVTQNIQFDFVKDKKYNKDALILKMQGFISSRTTYYNYKKTNHVKA
                     MRWPFQYNIGLKTNDKYVSLINYLPKNKIESTNVSQTLGYNIGGNFQSAPSLGGNGSF
                     NYSKSISYTQQNYVSEVEQQNSKSVLWGVKANSFATESGQKSAFDSDLFVGYKPHSKD
                     PRDYFVPDSELPPLVQSGFNPSFIATVSHEKGSSDTSEFEITYGRNMDVTHAIKRSTH
                     YGNSYLDGHRVHNAFVNRNYTVKYEVNWKTHEIKVKGQN"
     gene            complement(37293..38222)
                     /gene="hlgA"
                     /locus_tag="O2701_000237"
     CDS             complement(37293..38222)
                     /gene="hlgA"
                     /locus_tag="O2701_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB subunit A"
                     /protein_id="HCZ4292153.1"
                     /translation="MIKNKILTATLAVGLIAPLANPFIEISKAENKIEDIGQGAEIIK
                     RTQDITSKRLAITQNIQFDFVKDKKYNKDALVVKMQGFISSRTTYSDLKKYPYIKRMI
                     WPFQYNISLKTKDSNVDLINYLPKNKIDSADVSQKLGYNIGGNFQSAPSIGGSGSFNY
                     SKTISYNQKNYVTEVESQNSKGVKWGVKANSFVTPNGQVSAYDQYLFAQDPTGPAARD
                     YFVPDNQLPPLIQSGFNPSFITTLSHERGKGDKSEFEITYGRNMDATYAYVTRHRLAV
                     DRKHDAFKNRNVTVKYEVNWKTHEVKIKSITPK"
     gene            complement(38758..40068)
                     /gene="sbi"
                     /locus_tag="O2701_000238"
     CDS             complement(38758..40068)
                     /gene="sbi"
                     /locus_tag="O2701_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin-binding protein Sbi"
                     /protein_id="HCZ4292154.1"
                     /translation="MKNKYISKLLVGAATITLATMISNGEAKASENTQQTSTKHQTTQ
                     NNYVTDQQKAFYQVLHLKGITEEQRNQYIKTLREHPERAQEVFSESLKDSKNPDRRVA
                     QQNAFYNVLKNDNLTEQEKNNYIAQIKENPDRSQQVWVESVQSSKAKERQNIENADKA
                     IKDFQDNKAPHDKSAAYEANSKLPKDLRDKNNRFVEKVSIEKAIVRHDERVKSANDAI
                     SKLNEKDSIENRRLAQREVNKAPMDVKEHLQKQLDALVAQKDAEKKVAPKVEAPQIQS
                     PQIEKPKVESPKVEVPQIQSPKVEVPQSKLLGYYQSLKDSFNYGYKYLTDTYKSYKEK
                     YDTAKYYYNTYYKYKGAIDQTVLTVLGSGSKSYIQPLKVDDKNGYLAKSYAQVRNYVT
                     ESINTGKVLYTFYQNPTLVKTAIKAQETASSIKNTLSNLLSFWK"
     gene            40163..40267
                     /locus_tag="O2701_000239"
     CDS             40163..40267
                     /locus_tag="O2701_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292155.1"
                     /translation="MLTNRLTSVNYVKKRLIQNIQRILSKEVLYGEMQ"
BASE COUNT        13643 a         5861 c         7491 g        13675 t
ORIGIN      
        1 gcctgatcat catcttgtta tgttagttat ttataaccga ttttcaaaat aaaaaaccaa
       61 ccaagatgtt gaaatcaatg gttggttaat gtgtgtgtgt tgaaatcata tatggcactg
      121 tattttaaaa tatttaatga gatgactttg attcattatt tagtgactca cgcatcttcg
      181 tattgattcg tgttgcgcga cggaaataga cgctttcaaa tgaactcgtt gtaccgatgt
      241 aatagaagat aagtgccaca ataataataa cgataaagtc aaatggatag tgaatcatat
      301 ttaagccttt aaattctttg cttcctataa atgacagtat tgatagcacg attaaatata
      361 caataatcca taagctacca ccaatttgtt tctttgtatt acgccaattc atacgatatt
      421 catagaagaa gtagattggt aatccaagta taatgattaa aataacttca gccgttgttg
      481 gccacattgc ccaatatata gctaatgaag ctaatacaaa tgataatggt gccattactt
      541 ttaaaatttt tgctctaaat ggacgagtca ttgttggtcc catttttctt aatgcaatca
      601 ctgtcgttgg gccagttaaa taggctacta aagttgcagt agaaataact gctgctagcg
      661 taccccaatc tctaaataat gtaaccataa tcatactaat gatggcatta aagatgattg
      721 ctacacgtgg gatatgatat ttttcattca tcttcccaag aaatttaggg atatgtccat
      781 ttttctccat tgctcgtaaa actcgacctg taacggcgac aaatgacacg ccagtaccga
      841 atggtgatac aaaagcttca atgtatagta aaattgcgag ccaattaatt cctaataaga
      901 tagctaaatc agcaaatggt gaattgaagt tgatgccatt ccatccacta tgttgtaaca
      961 ttgattgagg catagaagtg ataaacgtac tttgtaaaat gatgtacaac actgcactga
     1021 ttgacagtga gatagcgatg cctcttgcga tatttttttc aggattttta atttctgaac
     1081 ccatattaat aattgtctgg aatgaattga atgaaaaaat aatccctgat gctgttgttg
     1141 cagcaaaaat cggtgcactt ccgtaaggca taaatgtgct tgccgaatgg ccgtaatttg
     1201 aagtgtcgaa tcctgatagc atcaacatga tgatggttaa catgggtacg cctaatttaa
     1261 atacagaaat taaactcgta aatgatgtta aaagttttac ggaccaatag tttaataatg
     1321 aaaaaataac aatgatgaga tatacagcta gcaatccgta tgtgctaata gagccatttt
     1381 ccattaaata tctcattggt ttcgcccaat gccacggcca agaactcata tattgaacag
     1441 ctgacacagc ttcgataggt attattgtca ccaaagacac ccaattcgcc caagcagcaa
     1501 taaagcctaa taatgagcca tgtgtatact gggcatagtt actcatgcca cctgattgag
     1561 gaaacattgt gccgatttca atgtagttat aggcaatggt tccaatgact aggaatccaa
     1621 gaacccatga tatgattgct gctggtccag ctattgatga tgcttcccac gcaccaaata
     1681 gccaaccaga tcctattaaa gagcctaacc ctaataagac taattgggat agattgattt
     1741 tgtcgttgtg caattgcttg tctttattca taaatactcc ttcgttattg ccaaaaatac
     1801 aaaaaataat atgcgacaat tatacgctta ttataataat agtcaaatgc attttttgta
     1861 agaacgcaaa agcatttgta aaattagttt tttataaaat taggatatat aatacaatgt
     1921 gtattgaaaa acaaaatgga agtgatgact atgagtgaga aacaatcaaa tgaacaagtt
     1981 gaagcatttt taaataaaga gagtcaatgg caagaagaat ataaatattt gagagcgtta
     2041 atctttaatg aaacagaatt agaggaagcg tataaatgga tgcatccttg ttacacgttg
     2101 aataataaaa atgtagtact tatccatggc ttcaaaaatt atgttgcact attatttcat
     2161 aaaggtgcca ttttggagga taaatatcat acactcattc aacagactga aaaggtgcaa
     2221 gcagctcgtc agttacgatt tgaaaattta acagagattc aagcacgtac cgaagaaatt
     2281 aaatattatc tagccgaagc aattaaagct gaaaaagctg gtaaaaaagt tgaaatgaag
     2341 aaaacagagg aatatgttat tcctaaagag ttagaagcta agttcgaaga aatgccacag
     2401 ttagagtctt cattttataa attaacgcca ggcagacaac atcaatatat atatcatatt
     2461 ggacaagcta aacgcagtga aacaagacaa aagcgtgttg aaaagtatat taaccaaata
     2521 ctagaaggta aagggatgca tgataagtaa ttaatgagta aagcataccg gttatacaac
     2581 aacatacaag atgacacgaa acaaccaatg gctcatgctg ttggttgttt ttttaggtgt
     2641 gtctgtcatg ggcaacactt tgacgttgga attccgttac aggcttggga gtagaaaatg
     2701 ttagcaaaag gcaagggtgt ctacaatgaa tgatgaagat attaaaatat aaggatgact
     2761 ttgtgagtgg cggatgggcg gttgtccgtc tgtaacaatg gatgcgtgtg cattattaca
     2821 aaaattcgac ttttgtaata atatttcaca ttttcgacac ttttttgcta taaaacaacc
     2881 aattgagcga taataaattc gcttttaaaa aatatgagtt atctatttag ttgccaaaga
     2941 taaaataata atgtttaata acatcatata gagtatgtta gttttaaatg tcgaatatac
     3001 gaatgtgcaa acaaagtaat cggtagaaat tcaacataca tagcgccgtt tactgttaag
     3061 tattcacatt acagatgaaa aatataaaat tctacataat caagaccatg atgtgtactt
     3121 gtttaactta tgactctatt tgtttaacaa ttgcgataat ggtcttttta ttttatgcgt
     3181 atcattcgtc atatttttta tgaggaagga gaaatgatta tgttaagtat taagcattta
     3241 acgaaaattt attctggtaa taaaaaggca gtagatgaca tctctttaga tattcaatct
     3301 ggggaattta tcgcatttat tggaaccagt ggaagtggca aaacgactgc tttaagaatg
     3361 ataaaccgta tgattgaagc gacagaagga caaattgaaa ttgatggtaa agatgttcgg
     3421 agtatgaatc ctgtcgaatt gcgtagaaat attggctatg ttattcaaca aattggctta
     3481 atgcctcata tgacgattaa agagaatatt gtgttggtac ccaaattgtt gaaatggact
     3541 aaagaggaaa aggataaacg tgcaaaggaa ttaattaaac ttgtggattt accggagtca
     3601 tttttagagc gttatccagc agaaatatca ggtgggcaac aacaacgtat cggtgttgta
     3661 agagcacttg cggccgaaca agatattatt ttaatggatg aaccttttgg tgcattggat
     3721 cctattacga gagatacgtt acaagattta gttaaaacgt tacaacgaaa attaggcaag
     3781 acgtttatct ttgtaacaca tgatatggat gaagcgatta aattagcaga caaaatttgt
     3841 attatgtcag aaggtaaggt ggtgcaattt gatacgccag acaatatttt aagacatccc
     3901 gcaaatgatt ttgtacgtga ttttatagga caaaatagac tgattcaaga ccgtcccaat
     3961 gacaagactg tagaaggtgt aatgattaaa ccaatcacga tacaagcaga agcaacactg
     4021 aatgacgccg ttcatattat gagacaaaaa cgtgttgatg ctatttttgt agtagatagt
     4081 aataaccatt tactaggttt cttagacatt gaagatataa atcagggtat acgtggacac
     4141 aaaagtttac gagacaccat gcaacaacat atttataccg ttcaaattga ttctaaatta
     4201 caagattctg tacgtacgat tttaaaaaga aacgttagga atgtacctgt cgtagatgat
     4261 caacagcgtt tagtaggact gattacgcgt gccaatgttg ttgatattgt atatgacacg
     4321 atttggggcg atagtgagga tacagtgcaa acagaacatg tgggggaaga cactgcgtcc
     4381 tcaaaagtgc atgagcaaca cactactaat gtcaaagtac gtgacatagg agatgataaa
     4441 tcatgattga gttcctacat gaacatggtg gacagttgat gtcgaaaaca ctggaacatt
     4501 tctatatttc tatagtggca ttattacttg ccatcattgt tgcagtacct ataggcattt
     4561 tattatcaaa aacaaagcga actgccaata ttgtattaac tgtggcaggt gtcttacaaa
     4621 ctattccaac actagctgta cttgctatta tgataccgat ttttggtgtt ggtaaaacgc
     4681 ctgcaattgt agcgctattt atttatgtat tattacctat tttaaataac acggtactcg
     4741 gtgttcaaaa tattgatagc aacattaaag aagctggaaa aagtatggga atgacacaat
     4801 ttcaattgat gaaggatgtt gaattgccgt tagcattgcc gcttatcatt ggtggcattc
     4861 gtttgtcatc tgtgtatgta attagttggg ctacacttgc aagttatgta ggtgcgggtg
     4921 gattaggtga tttcattttc aatggtttaa atttatatga tccactgatg attgtaactg
     4981 caacggtact cgttactgca ctagcattag gtgttgatgc cttattagct ttagttgaaa
     5041 aatgggtagt tcccaaaggc ttaaaagtat ctggataatt aggaggctaa gataatgaag
     5101 aaaattaaat atatacttgt cgtgtttgtc ttatcgctta ccgtattatc tggatgtagt
     5161 ttgcccggac taggtagtaa gagcacgaaa aatgatgtca aaattacagc attatcaaca
     5221 agcgaatcgc aaattatttc acatatgtta cggttgttaa tagagcatga tacacacggt
     5281 aagataaagc caacattagt aaataattta gggtcaagta cgattcaaca taatgcctta
     5341 attaatgggg atgctaatat atcaggtgtt agatataatg gcacagattt aacgggagct
     5401 ttgaaggaag caccaattaa aaatcctaag aaagcaatga tagcaacaca acaaggattt
     5461 aaaaagaaat ttgatcaaac gttttttgat tcgtatggtt ttgcgaatac gtatgcattc
     5521 atggtaacga aggaaaccgc taaaaaatat catttagaga cagtttcaga tttagcaaag
     5581 catagtaaag atttacgttt aggtatggat agttcatgga tgaatcgtaa aggcgatggc
     5641 tatgaaggat ttaaaaaaga gtatggtttt gactttggta cagtgagacc aatgcaaata
     5701 ggtctagtct acgacgcatt aaactcagag aagttagacg ttgcattagg ttattctaca
     5761 gatggtcgaa ttgcggcgta tgatttgaaa gtacttaaag atgataaaca atttttccca
     5821 ccttatgctg cgagtgctgt tgcaacaaat gaattattac ggcaacaccc agaacttaaa
     5881 acgacgatta ataagttgac aggaaagatt tcgacttcag agatgcaacg cttgaattat
     5941 gaagcggatg gtaaaggtaa agaacctgct gtcgtcgcag aagaattttt gaaaaaacac
     6001 cactattttg ataaacagaa aggtggtcat aagtaatgga aggtaattta ttacagcaat
     6061 tattcaatta ttatgttacg aactttggtt atctatggga tttatttttc aaacacttat
     6121 taatgtctgt ctatggtgtg ctgtttgcag ctttaattgg tattccattg ggaatcttgc
     6181 ttgcaagata cacaaaactt tctggatttg taattacaat tgcaaatata attcaaacag
     6241 ttccagtcat tgcaatgtta gctattttaa tgttagtcat gggcttaggt tcagaaacag
     6301 tagttttaac agtgttttta tatgcgttac ttccaattat aaaaaacact tatactggta
     6361 tagctagtgt tgatgcgaat attaaggatg ctggcaaagg tatgggaatg acacgcaatc
     6421 aagtgctacg aatgattgaa ttaccgttat ctgtttcggt tattatcggt ggcattcgta
     6481 ttgccttggt tgttgcgata ggtgttgttg ccgttggatc atttatagga gcacctacgc
     6541 ttggtgacat tgtgattcgt ggtacaaatg cgacggatgg cacaacgttt attttagcag
     6601 gtgcgattcc gattgctatc attgcaatcg tcattgatgt actattaaga tttttagaaa
     6661 aacgattaga cccaacaaca cgacatcgta aaaatcaatc taatcatcgg ccgcaaagta
     6721 ttaatatgta atagtagaag atgtttataa tttagcgatt tcgtttcatg atttataaaa
     6781 aatgaggcta ctcaaggagc tcaaataatc tttgagtagc ctttttatag gttgtgtttg
     6841 tatgcgttta cactaaaata gcaattatta tcatgaaagt ttttggataa aaagcgttaa
     6901 ttattgtaaa aatactaaaa aatgagatgt tttatttata attttctgca aatttatgat
     6961 attgtttctt aatatatcat attaaaaatt tgtttttctt aaacatagga ggcttatcta
     7021 attcatggac acatcaaaac aatttagagg tgacaaccga ttgcttttgg gtatcgtttt
     7081 aggggttatt accttttggc tattcgcgca gtcacttgtt aatcttgttg tcccattaca
     7141 atcaacatat agtagtgacg ttggaacgat aaatatcgct gttagcttat ctgccttatt
     7201 tgctggtttg tttatcgtag gtgctggtga tgttgctgat aaatttggtc gcgtcaaaat
     7261 tacttatgta ggattgatat taaatgttgt aggttcatta ctcatcatca ttacaccttt
     7321 gccagcattt ttaattatag gtagaataat tcaaggtttg tctgcagcat gtattatgcc
     7381 atcaacactt gctattatta acgaatatta tattggtaca agaagacaac gtgccttaag
     7441 ctattggtct attggttctt ggggtggtag tggtatttgt acgttgtttg gtggcttaat
     7501 ggctacatat ataggttggc gttcaatatt tgttgtttca attctattaa cattattagc
     7561 aatgtactta atcaaacatg cacctgagac taaagcagaa ccaatcaaag gtatgaaagc
     7621 agaagctaaa aagtttgacg ttattggttt agtcatttta gtagtgacga tgttaagttt
     7681 aaatgtaatc atcacacaga cgtctcattt tggtttagtt tcaccgttaa ttctaggttt
     7741 aattgttgtg tttatctatt cattagttgg ttttgtatat tacgaaaata aaataaagca
     7801 tccacttgtt gatttttcaa tttttaaaaa tagaggatac agtggtgcaa ctatttcaaa
     7861 cttcttatta aatggtgtag caggtggtgc acttatcgtt attaacacgt attatcaaca
     7921 acaattagga tttaattctt cgcaaacggg ttatatttca ttaacgtatt taataacagt
     7981 gttgtcaatg attcgtgtag gtgaaaagat tttatctcaa catggtccga agcgcccact
     8041 attactagga agtggcttta cagtgattgg gttaatctta ttgtcgttaa catttttacc
     8101 agaagtgtgg tatatcatat ctagtatagt tggatattta ttgtttggta ctggtttagg
     8161 attatatgct acaccatcaa ctgatacagc agttgctagt gcgccagatg ataagtcggg
     8221 tgttgcttca ggtgtgtata aaatggcgtc atcattagga aatgcatttg gagtagcagt
     8281 atctggtacg gtttatactg tgttagcagc taatttaaat ttgaacttag gtggtttcac
     8341 aggtatgatg tttaatgcct tgctagcaat tgttgcattt ttagtcattt tactattagt
     8401 tcctaaaaat caaacgaatt tgtaaaactg aaatgaaagc aagttattat gtagggattt
     8461 taaaggaaat tttgtgaaag taagtttatc atacacactt aatgttgcgt attgacgttt
     8521 aatgttaggt gtgttctttt atagacgata aaagctgtgt gcatattaag cgaatgattt
     8581 tcaaattgac gctaatatgc gaaagtagta tttttaaaat gaacaacaac gatgaagagg
     8641 ggtttatagg atgaaaattg caattgctgg atcgggtgca ttaggtagtg gctttggtgc
     8701 caaactattt caagcaggat atgatgtcac acttattgac ggatatacat ctcatgttga
     8761 agcggttaag caacatggat taaatataac gattaatgga gaggcattcg agttaaacat
     8821 tccgatgtat cattttaatg atcaaccgga cgaaagcatt tacgatgttg tctttctatt
     8881 tccaaagtct atgcaattaa aagaagtgat ggaagatatg aagccacata ttgataatga
     8941 aacgatcgtc gtatgtacga tgaatggtct gaagcatgaa gaagtcattg cgcagtatgt
     9001 tgctcaatca caaattgtca gaggtgttac gacttggacg gcaggtcttg aaagccctgg
     9061 acacagtcat ttacttggta gtggaccagt tgaaataggt gaactagtgg atgaaggtaa
     9121 agaaaatgtt ataaaagttg ctgatttact taacgaagcg gaattgaatg gtgtcattag
     9181 taaagattta taccaatcga tttggaaaaa gatttgtgtt aatggtacgg caaatgcatt
     9241 aagcacagtg ttggagtgta atatggcatc gctgaatgaa agtagttatg cgaagtgttt
     9301 gatttataaa ttaacgcaag aaatagtgca tgtagcgacg attgataatg ttcatttaaa
     9361 tgttgatgaa gtatttgaat atttagttga tttaaatgaa aaagttggtg cgcattatcc
     9421 atccatgtat caagatttaa ttgttaataa tagaaaaact gaaattgatt atattaatgg
     9481 cgcagttgca acattaggta aacaacgtca tattgaagcg ccagtcaatc gctttattac
     9541 tgatttaatt catactaaag aaagtcagcg acacgcacag gattaattgg tgttttaggt
     9601 atgattacaa ggaaatgaca atcacgtgat attacggtca ttattaagat tgaaatgtaa
     9661 taaataaaga acagcagtaa ggtactttca aattgaaatg atcttggtgc tgtttttctt
     9721 gattgatctt cgtcataatt cagatttgtc ataggtctac gacatactat tagtatttac
     9781 tagacagttt ttacgacgac actttgaaaa attttgaggc aaatcatttg gaagtctcac
     9841 gtgaattttg taaactcatc aagcaagtaa ttatattaaa aagacaaata gagaaaaggt
     9901 gtttataatg agtaaaattt ttgtaactgg tgcaacgggc cttattggca ttaaattagt
     9961 tcaaagacta aaagaagagg ggcatgaggt tgctggtttt actacatctg agaatggtca
    10021 acaaaagcta gctgctgtta atgtaaaagc atatattggt gatatattaa aagctgatac
    10081 tattgatcaa gcgttagcag attttaaacc agaaatcatt atcaatcaaa ttacggattt
    10141 aaaaaatgtt gatatggcag caaatacgaa agtacgtatt gaaggttcta aaaacctaat
    10201 tgatgcggcg aaaaagcatg acgttaagaa agtaattgcc caaagtattg cctttatgta
    10261 tgaacctggc gaaggattag caaatgagga aacttcactt gattttaact caactggcga
    10321 tagaaaagta acggttgatg gtgtggttgg tttagaagaa gaaacggctc gtatggatga
    10381 atacgttgtt ttacgttttg gctggttata tggcccaggt acttggtacg gaaaagatgg
    10441 catgatttat aatcaattta tggatggtca agtgacactt tcagatggcg taacatcatt
    10501 tgtgcatctt gatgatgcag ttgaaacatc tattcaagct attcattttg aaaatggtat
    10561 ctataatgta gcagatgatg cacctgttaa aggttctgaa tttgcagaat ggtataaaga
    10621 acaacttggt gttgaaccaa atattgatat tcaacctgcg caaccatttg aacgtggcgt
    10681 aagcaatgag aagtttaaag cgcaaggtgg tactctgatt tatcaaactt ggaaagatgg
    10741 catgaatcca attaaataat aatttatccg tttaatatac aaagaataaa gacttggtcg
    10801 aatcgtggat gatatattat caaacgcacg gctcgaacaa gtctttttta ttatgtcttc
    10861 gttatctttg tatgaaggaa taacagaatt acaattaatg tactgaataa tgcaattaat
    10921 gttgtgatta gtgctaattt aatttctatt ggtagccaag tcagtacaaa agaccaatta
    10981 ttgctaccga gaatgagata tggtaatgca tataatatga gcgctaaagc gatacatata
    11041 cataatgata accaactcaa tacagcaatc cgttttgatc tacgcaaaag aatttgacca
    11101 tggcgcattt gccaagcacg ataagctgag aatataaatg ctaataaaat aagtgttgtc
    11161 atcaaaacgg taacaatatt aaattgatct ttatattgat tgagcataga cgcaaccgtc
    11221 gaatatcgct tgtgatttac aatttgtgta ttaagatgct caactaattt gggtacatat
    11281 tccgaattta gatttgcaag tacaacaatt ccataatttt gttttggatt tagtaaaata
    11341 aatgatgaaa agttatctag cgttcctgaa tgaaacacta aatgttcatc attattggta
    11401 aaccagccgg aagcatatgc attggcatta ggttcaccaa ttgttgaaga taaattttta
    11461 tgtgattgtt gaactaatga tttgtattta tcaggtggat taagttggaa ttttatccaa
    11521 tgttccaaat cttcagttga tgtcatcata tatgctgatg gtgtatccca aaggttaaat
    11581 tcaggtttag agacgacagg tgtcgaacct tgtaattcat agccaatagc atcatgtttt
    11641 gatttgtaat tggtttgttt gaatgatgta tgtgtcatat gcaaaggctt gagccatgaa
    11701 tttgtaatat attttgtata ggattgcttc gtaacgtttt ggataattaa acctaataaa
    11761 tcatagttca tatttgagta ttcaaattct tctccgggct tatgatgtaa ttcatcaccc
    11821 ataattgcac gggttacatc atttaaacga ttatttttgc ttgtcacaga atcttcgctt
    11881 gtaatatcac taggtatacc acttgtttga gccaaaagtt gcttaatcgt aatagtttca
    11941 ttttgaccat tatagttcat tttaaaatga ggcacatgtt tggatacggc atcatttaag
    12001 tttaatcgac cttcttgagc taattttaaa attgcaagac ctgtgaaagc tttcgtatta
    12061 gaagcaattt catactttgt tgtgggagaa gctttgactt ttttatcaac atcttgataa
    12121 ccataacctt tatttaaaaa aactttgcca ttttttacta ttaaaattga tgctccagga
    12181 atgtgtccct tttgtaaatc atgctcgata attgtatcta tttgttgttg cgaatcattg
    12241 gttaaccgtg tcttcgtatt gctatttaat gttatatata tagcaattga aatggcgact
    12301 aaaatgataa tagaaatgga tagaaaatac agttttttag tagtcataaa cgttcctttt
    12361 tatatgttaa atttacttaa gtgcctaatg agatttattt tattatacca gttctattga
    12421 ataaattaat taaagaggta tatttaccat tatttttgac accttatatg ttaataggtg
    12481 tataattaca taaaaaatat taattaactc tctatacttt ttggaaagat aggcgtatag
    12541 tgatatccat caaaaactat aaaaaggaat aggagattat gttcaatcaa tttaaaagac
    12601 ttattatagg gcaacctaaa aaaaacagag aactaaaaga cgaaaagata tcgaagttta
    12661 aagggttagc gattctttcg tcggatgcat tatcttcagt ggcttacggt cccgaacaaa
    12721 tactgattac actatcagta gtgggtgcag ttgcaacttg gtatacttta ccgattgcag
    12781 gtgctgttct gatcttatta gctgctttaa ttatgtctta taggcaaatt atttatgcat
    12841 atcctaaagg tggcggcgcg tatatggtgt ccaagaccaa tttaggagaa aaatggggat
    12901 tgcttgcagg aggctcatta ttagtcgatt acatactgac tgtcgctgtt agtatatcat
    12961 ctggtgctga tgcatttgtt gctgcattcc caagtttata tgggcataaa gtactgattg
    13021 catgtttact tgtacttttt attttaatat taaatttacg tggtttaact gaatctgcca
    13081 ccgtattatc ctatccagta tatcttttca ttatagggtt agtgatatta atatttatag
    13141 gtactttccg tgtggcgaca ggagatattc aaccacatat gcatgcatca gtaggaactg
    13201 cggttcctgg agtaacatta ttcttgctat taaaagcatt ttcatcaggg gcttcatcgt
    13261 taacgggtgt tgaagctatt tcaaatgcgg taaccaattt tagagagcca agtgctaaaa
    13321 atgctgtgaa aacgttaatt gctatgggtt caattttagc gtttttatta gttggtatcg
    13381 ttggtttagc atatgtttat ggaattttgc cacaaacgga aacgacagtt ttatcacaat
    13441 tagctatgca aatatttggt gataatgctg ccttctattt tgtacaggca acgaccgtga
    13501 tgattttggt attagcagcc aacactggat ttacagcttt tccgatgtta gcggcttcaa
    13561 tgtctaaaga taaatatatg cctagaatgt ttactgttag aggtgatcgc ttaggctact
    13621 ctaactcgat tattatttta ggtgtattag ccattatttt aattattgta tttgatggta
    13681 tgactgaaga tttaattcca ttgtatgcgg ttggtgtatt tattccattt acactagcac
    13741 aattcggtat ggttattaaa tggattcatg aacgtcctaa gaattggtta agtaagttgt
    13801 ctgtgaactt acttggtggt atcgtgacat ttattgtttt tatgatttta ctcataacta
    13861 aatttagcca agtatggccg atacttattt tcttgccgtt cgtggtgatt ttcttcttga
    13921 aaattaacaa acattatcga gatatcgcag aacaattacg ttctgacatt gatgttctta
    13981 atgtggacgt ggtagatcgt aatttagcaa ttgtgccgat aacaagtatt acaacagcag
    14041 ttgataagtc tatttattac gcacaaatgc ttgcgaataa tgatgtcata gcagtacatg
    14101 tatcatttgg agatgaagat gaaaaagcat tccaagagaa atggaaacgt catttcccag
    14161 atgtaagatt agttatttta cattcggaat atcgcagtat tatacgacca atttcacgtt
    14221 ttatcgataa gattaatcgt aaagccaacg atcaaaacta tatgattaca gtagtgatac
    14281 cagaatttat tactaagaaa cgttggcata atttattaca caatcaaact agtttgcgta
    14341 tgaaattgta tttaatttat caaaagaatg ttaacgtatg tacaattcca tttaaactta
    14401 aaaaatagat tctataatca aatcggattg atgaaaggtt atttactttt catcaatctg
    14461 attttttgat gttgaatata aaaaccccgt acatatgaga tgataatgac tttgtctcat
    14521 atgtacgggg gtattgttat gtcttgctta atgtttggac tgtaaagaca agacaatttc
    14581 gttgtaattg atattttctc gaatttgagc tgcaacgata ttgttgattt tatttttgcg
    14641 aattaattca tctaaaagat gacgttgtaa gtacaatgct tccaatttgt ggttatggaa
    14701 catagcttgc ggtatgattt gattgctgtt ttcttgacgt tcttttcgac gttgtgcacg
    14761 ccactttaat gagcgactca tatcatagta atgattaatg actaagctaa cctcaagtac
    14821 atttgtacta ttttgttctt cttttaagcg taaaataata ttgtgattca caatacgcat
    14881 aattttttga acatctttga atgaattttc aaattcttgc ttcttagatt ctttatttaa
    14941 ggctttgtag tgcttcatcg ctttaagtct aagccataac atcttgaaga agtttgcaaa
    15001 tttttcaaca aatgatgcag tacggtgtgt ctctgttaat tcgacaatat tgcggtaatt
    15061 attaatatcc tgatatgttg ccttaccttt atcaattaaa cgctcaagtg tagatgtttc
    15121 gattactttt gcaatatctt ctaaacgttt gagttctttt gtattttgat tatcaggttc
    15181 tgaatttaat aaaaataaca attctccata gtattggttt aatactgggc gataatttgt
    15241 atcgtttttg tcttttttac tttcgttttt aaaatgctgg atcacttttt gaacaatgaa
    15301 aattttggca gattgataag tcataccttt aaaagtagta tcttcggcag atggtgtaat
    15361 taaaggtaaa acaatttgcg ctaagattaa actaattaat accatgaaag atgcaataaa
    15421 caataaatca ttacggtatt cgaatgcttg tccttttgta atgataaatg gtaatgtaag
    15481 tgccattgaa agtgaaattg taccgtgaat accacacatg gtcataataa atgcgtaacg
    15541 cactcgagaa ggtggtgttt catgtgaatc atgttcctcg tctagataag attgtatatt
    15601 tttcgggaaa taaaaatctt tgtaccagaa ataaacccaa acaaatcgac atgcataaat
    15661 tgcaatagcg attaatatag tgatcactat taaaaatgaa aagttgtcag gctctgtttg
    15721 gaaaatatct atgactacag taggaatcat aaaacctaat acaacaaata caaaaccatt
    15781 taaggcataa ctaaatgtgt tccaaatttg atggtaattc atttgtagtt ccgtttgtgc
    15841 tctaattaaa cggtcgcgtt ctaaaccatg gattagacct gcgattacaa ctgcaatgat
    15901 acctgaagca tgaacttctt ctgctaaaaa gtatacgaca aaaggagtta ataattgaat
    15961 aaaagttaag gtattgttat cttttaaacc tttattagcg gttaagtcta tacgtattct
    16021 aacgacaacg aatccaataa ttgcaccaat aagtacacct agtattgttg aaatgataaa
    16081 ttgttcaaca gcttggaata atgaaaaggt accagttact aatgcagtaa cagcaatttt
    16141 aaatgaaatg atacctgctg catcattgag taaagattca ccttctaaaa tcgtcataga
    16201 acctttaggt aataattttc cgcgtgtaat agcagatact gctactgcat cagtaggaca
    16261 taaaattgct gctattgcaa aagcggctgg cattggtaag gcaggccaaa tccaatgtat
    16321 aaaatagcca acaccgacta cagttgcaaa cactagtgcc attgacatta atagtatagg
    16381 tttacgatat tctaataatt ttgttcgaga gacgtgggta ccttccacaa aaagtagtgg
    16441 cgcgataacg gcaaacataa atacttcaga attgaattgg aaatcaactt gtattggaat
    16501 aatgaaaata acgacaccta atgcaatttg aataaaggca gtaggaattt gtgggaatcg
    16561 attattgata accgaactaa taatcacagc aaaaataaaa attaaaaatg cttctaatag
    16621 tgccatacaa tactcctcaa aattttaata gttaatattt tatcactttt aaggcataat
    16681 gacatagata tattgataaa atgaagttat tttcaaaaaa actctagtat cggttgaact
    16741 gatactagag cgagatgttt aaattattga ttgtcatatc tgaaatgacc gctgtcattt
    16801 tgtcgttgtt catacgcgag cttttcagca ttcgttttgt attttttata aaagaaaaat
    16861 aaaaatataa accagaatgg cgaaatataa atagctgctc ttgtttcgtc actaaagaat
    16921 aataaaatga atacaaagaa gaagaacgct agaataatgt aagcaatagg cttaccacca
    16981 atcaacttaa atttactgtt tttatgtgcc tcaggatgct ttttcaaata cattagataa
    17041 gcgacaataa tcattgccca aacaactaaa aacaacacag ttgataacgt tgtaacgtat
    17101 atgaatagtt gaattgcatt agggaaaatg tagtttaaca aggctgctat aagtaatgca
    17161 attgatgaaa ctaacattga taaataaggc acgccatgac tattcgtttt atttaaaact
    17221 ttaggaccta acccttgttg tgacagtccg aataagatac gactattcga atagatacca
    17281 ctatttgtag cagaggccgc ggcagttagc acgacaaagt taacgacacc tgctgcaaat
    17341 ggtacgccga ttaatgtaaa gagtttaacg aaagggctac tatttggatc gatatcattc
    17401 caaggtatga ctgacataat tactaataga ccaccgatat agaataataa aatacggata
    17461 ggtacattat taattgcttt cggtaaggtt ttttcaggat ctttcgtttc accagcagtt
    17521 acacctataa gttcaatacc aatgaatgaa tatacagcaa tttggaatga cattaagaaa
    17581 ccaaatgttc caccagggaa catgccaccg tgactgatta agtttgtgaa tgatgcatgt
    17641 ccataatgtg ttttaaatga gaaaaagatt aatacaagac caacgataac catcgcaata
    17701 attgtgacaa ctttaataat cgagaaccaa aactccagtt caccaaataa tctggcacct
    17761 aataagttga agctaatcaa gattaaaaca ataaatagca cggtaatcca atttgggact
    17821 tgtggatacc aaaatgcaaa gtattgtccc atcgcagtta ggtctgacat acttgataca
    17881 atccaacata accagtaagt ccagccaatg acaaagccac caaaagggcc taaatattca
    17941 tttgcaatat caacaaatga attaaatctt gtattgctca acaacaattc gcctaatgcg
    18001 cgcataaaag cgaatagtac aaccccaata atcatgtatg tgaataacag tgatggacca
    18061 gttaaagata ttgtttgacc tgatcctagg aataacccag ttccaattgc gcccccaatt
    18121 gctattaatt ggatgtgtct gttattcaac tctctatgca attttcttgc cataattttc
    18181 ctcctcagga taaagcaaat aaaaaagttg tgtgtaagcg ttttcttaat tataaatact
    18241 aaagaaatcc atttaataaa gcaattattt ttttaaatat aagaaaaaac atttgatagg
    18301 taagggtatg ataactaaaa cttatcgatt ttttgaaaag atataagttt ttatgcaaaa
    18361 atataaaata atcatctaaa tgaagcagtt aactatatat attagataag ctatggcata
    18421 ataaataaca ttttaatttg agtaaagaag gtaagggtat gaagataggg acaatcgccg
    18481 atttacatat tgatcgtcat aataaaaaaa catccgaaga ttacttagag gcattggttg
    18541 aaatagttaa gtataagaaa ttggatatat tgttgattgc gggggatatc tcaaatcatt
    18601 atcaattgac gcaccaattt ataacgcaat tgacaaaaca attagatata cctgtgaaat
    18661 ttgtacctgg aaaccatgat ttatgggaag ttgaaagtat gactacgcaa gacatttgga
    18721 ataattataa gagtatgtca cagtgcttgg taggaaaacc atttatagta aatgaagaat
    18781 gggcaatcat aggacatact ggctggtatg attatagctt tgcagcacaa cgattttcat
    18841 tagatgagtt acaaaaagga aaacattatg gtgcgacttg gcaagataaa gaacgaatat
    18901 cttggggcat atcagatcaa aatttatcta aaatagcggc tgaacaagtg aagaaagata
    18961 tattagaagt aggaaataga cgagtgattt tagtcacaca tgttgtgacg caccctgatt
    19021 tcattgttcc tatgccgcat cgtatattcg atttttataa tgcatttatt gggacaagtg
    19081 atttcaatcc tttgtatgcg atgttcgata taccatatag tattatgggc catgttcatt
    19141 ttcgtaaaag tgtgatagat gatggcagat gttatctctg tccgtgtcta ggctatccaa
    19201 gacaatggcg ttcagaagat atttaccagg aaataaatga gacgatacaa ataatagaaa
    19261 tttaaaatgc gcaaacctga cccagtttgc gcattttatg ttttacacac gcgagtaatg
    19321 tgtttactta cgtgtgttta ttttgttgct gattttcaat tgtatatgaa tgtggttgca
    19381 cataaatgca ctttcttcct ggtgaattaa agctgtattc cattttctct ttacgaattt
    19441 taataatttg tttgcgattt ggaatgatgg caggtaaaac taggccacga cgaatatgac
    19501 tccaaagtgg atcgatataa tagtaatagt aatcatcgta cccaatgagt aacgttacgt
    19561 gaatatttga aacttgtttc gctggcttgt tatcaaagcg gaaaacacgt agtaatggtt
    19621 tagaaccaag attagtatgg tatattaaca caggttgacc ttgatcgata atacctttaa
    19681 gatcttctaa cgatttacca gtgccgtcta cgatattagg attgtatttt tgtaaaaatg
    19741 gtacatatgc ttctggaaat atcgtttgat gataattgcc aagcttaatg aataagtgat
    19801 gtccaacata acctttatgt ggattgttcg gatgtgtcgg ccaatgtctc ataatttctg
    19861 tagcagggat atgttggttg ttgtattgca acatcatggc tgcggaaaca ccttcacacc
    19921 ccatgaccat agggatagga aatagctgac tgataggttt aactggtaat atttttcggt
    19981 tcataatata gtcctcgcat tgattcaata aatatttaat ataattatat agcgtcaatg
    20041 caaaatgtcc taaacatatg ttttacatga gtgaataaaa ttaatggagt gataaaatgg
    20101 aatatcaatt acaacaactt gcgtcgttaa cgttagtagg tattaaagaa acgtatgaaa
    20161 atggacgaca ggctcagcaa catatagcag ggttttggca aagatgttat caagagggag
    20221 taattgcgga tttacagtta aaaaataatg gtgatttagc cgggatactt ggcttatgta
    20281 tacctgaatt agacggtaag atgtcatata tgattgcagt taccggagat aatagtgctg
    20341 atattgaaaa atatgatgtc ataacattag caagttcaaa gtatatggta tttgaagcac
    20401 agggcgcagt acctaaagca gttcaacaaa aaatggaaga ggttcatcac tacatacatc
    20461 aatatcaagc agatacggta aaatcagcac cattttttga gttgtatcag gatggtgata
    20521 ctacaagtga aaaatatatt acagaaattt ggatgcctgt taaagggtga ttgaatagaa
    20581 atgtccttgc tatgaaggcg taattttagt tgaagattcg atctcataat tcataaagaa
    20641 gaaccctgac aaagatagat atctttgcta gggttctaat tatgttgaag tgttatgcga
    20701 catgttgctt tttaatcatt ttaaagtaaa tgatttgtag actgactaat agaatggctg
    20761 taatgaagat aataatcata tatggtgatg tattaaattc gccttttaag ccaactaatg
    20821 gtgcaacagc gccacctaag ataaattgga ataagccaag taaacttgat gcgttaccac
    20881 tgccacctgt tcgttcttcc atagccattg tgaaaccaag cggtccaatt gacgtcacag
    20941 gacacacatt taagaagaat gcgatgagta agacccaaag tggtaaatgg aatgtaagtg
    21001 tcaggataat taaagcaaca cctaccactt gtataatagt taaaatgatt aataatatgt
    21061 ggcgatgtaa tttttctact aataaagcaa cgacttgact gacaatgatt aaacctacac
    21121 cgttaacagc aaacatgata ctaaattgtt ggggtgtcat attatacaat ttttgagtaa
    21181 taaatggcga tgcagatgaa taactaaata gcattacata agttaaacct tgtaatagca
    21241 ttggaataat aaatgctggt tttttcaaaa gacttccaaa atctttaatg acgctactaa
    21301 aattcacctg ctttaattca tctttagatg ttttaggtaa ttgagaagcg acgccaatta
    21361 aaatgatgag tgccacaata gttaaaattg tgaaaataga acgccatgtt gctacggata
    21421 aagctaatcc accggctaat ggtgcaagaa tagtgatgat gccattaact accattaaag
    21481 atgctaaaaa tttagcgagt gcgttgccac taaatttatc accagcagag gcttttgcaa
    21541 tcacgatgac gccaccacca gttaaacctt gaataaatcg taaagttaag aataatggca
    21601 attgatcaac aaacatagac aaacctgata ccaaaatgaa aatgatcaaa atggatacag
    21661 caatccgttt gcgaccaaaa gcatcggata aaggtccaaa gataaattgg cctagtgcaa
    21721 gaccaatcat tgtgaatgat aatgttaatt gaatttctga tgtagtagaa ccaaaatcac
    21781 cttgcacttt aggtaatgat ggtacgtaca tatctatagt tagtgggcca aaagcggtca
    21841 tgacgcctaa cgtaattagc aataaaaatg aaaaatgctt cgttttggtt ttcgttgatt
    21901 gcatgatcag actcctttaa atgatataat tatataaatt atatacgttt tgtgtgtatt
    21961 tgtatgtact tagaatacaa gtttatataa aaaatcccta tactcttaat gcatcatcac
    22021 gaataacgta gtgatacaga taaagtatag ggaattaatg tttatcatca aagcaggtgt
    22081 aataattaat tagcgccacc attgtcatct ttaaataagc tttctaattt agatttaata
    22141 ctgtcgaatt ttgtattaat atcatttaac atgctttcaa ttttaccttg gcttgcattg
    22201 tttttaagtg cattatttgc ctctgtcaat ttgttgattt ggtcttgata aaattgagtt
    22261 agcttagaat cagctttgtt ttgttcttgc tgcttcaagt tgtcgatttg attttgtaaa
    22321 tttttaataa tgtcatcagt tttttggttg tttttaatat tttctttagc attatctact
    22381 tgctggttaa tttggccgtc ttgattttca acttgttttt tataattttg agagacttta
    22441 gggtcattac taactttatc gctatcattg ttaagtaagg cgcgtgtgac aaaaatagcg
    22501 acaataatta agagaatggc aataatgata tataaccatt tttttgaacg gcgggatttc
    22561 tcgttttctt cttcaagaat ttgttctctt cgataatctc taccattata gtatcgttcc
    22621 gggtgttctt caggatcgcc tttagcatat tgttgtctat acgatgcgtc ttcagattga
    22681 cgacgatgat tttggttttg attgtcgtat tgatatgaat cacggtattt atcagttctt
    22741 ttcatgggtt tcacctcatt caagttttaa ctgcaaataa taaagctgat gatttacttt
    22801 gacattattg taatttcaga atacgagcaa tgtcagttgc agtgtttaat aaattttgtt
    22861 cgctattttg taggacatct tctaaagtgc taggtgaaga gattatagaa taggcactat
    22921 caataccgaa atcgtaaaca tgttgataat tttcgcctag actgccacaa atcgcgatga
    22981 caggaatatg atattgtttt gcagctaacg caacgcctac gggtgtttta ccaaagatgg
    23041 tctgataatc catgcgtcct tctccagtaa taacgaggtc tgcatcttta attctttgat
    23101 gaaaatctgt aatgtcaaag acgacatcaa taccttttgt taaagttgtc tcacaaaacg
    23161 ctaataatgc tgcgcccata ccgccagctg caccagaacc tggtatttga ttaacggact
    23221 ttcctgtgca catttttatc ttatcatgat aatgcgacat tgcgaaatcc aactttggta
    23281 tcatctttgc atcagcgcct ttttgaggac cataaatata ggtagcacca ttttcaccca
    23341 ataaaggatt tgaaacatca caggccactt taaaggtcac ctcttttaat cgcgaatcta
    23401 gattggttat atcgatttgt gcaatgtgag caagattagc accattcatt tgtaataagt
    23461 ccccgtttac atcagtaaac tttacgccta gtgcacttag catacctgta ccaccatcat
    23521 ttgttgcact gccaccaatc cctaaaataa tggtcttagc accatgattt aatgcatctt
    23581 taattagttc accggtacca tatgatgatg tgtataaagg attacgttcc tctttttcta
    23641 ataaatccaa acctgacgct gccgccattt caataattgc aatttgttgt tcgtctgcgc
    23701 gtgcataaca tgcttcaata ggtcgcatta aagggtcatt aacgatgact gtatacttag
    23761 tggcacctgt tgcatgcatt aaagcatctg tggtaccttc accaccatca gccatcggaa
    23821 tgatatcata atgaagggta ttcccataaa cattagtaaa agcctgtttt ataatattgc
    23881 caacttgctg tgcggtcata ctttccttaa atgagtcagg agcgatgaca attttttgta
    23941 attgcatagt agacatcctc tcattactgt tgtcgctctt attgtatata aaattactag
    24001 tgaactaaag taatctctat agtgctaatg agtcacgcga taaatcagtt acattataat
    24061 ggcgtataag gatgagggga aagagactaa atttgctgtg aaagggtata aagattgaaa
    24121 taagcatatt tgaagtgaag cggattggct cgcatattcg agtatgttcg agaacgtagt
    24181 tatgcatata ttttgatgtt gaggagttac acatatgaaa ctaacacaaa cgcatgctga
    24241 aattttaaaa tttatcattg ttggcggcat taatacgtta aattattatg ttgtctattt
    24301 attgctgtta aaattattac acattgaata tatgattagt catattaccg gatttctagt
    24361 cgcttttgtg atttcatatt atttgaattg ttattttgtt tacagggtaa aacctacttg
    24421 gagaaaattc attagttttc caattacgca gattgtcaac gtaagcttac aaacagtttt
    24481 attatatgta tttgtatcct ggctaaattt gccagcagaa attgcaccat ttgctggttt
    24541 aattattacg atacccatca catttatatt atctaaatgg attttaaaag atagcaatta
    24601 aagtagcgta aaccactaaa aaaaacttta cttaagcctt aatattgcgt gaaatataat
    24661 aaataaatga tataatatga tggtcgccta acatctcggg cgttacttta acgcagagag
    24721 gaggtgtatg acggatgttc aatttattaa ttaacatcat gacttcagct ataagcggct
    24781 gtcttgttgc gttttttgca cattggttac gaacgcgcaa caataaaaaa ggtgacaaat
    24841 aaccaacacc taaataaatc ccctcactat ggcagtagtg aggggatttg gtgtatttag
    24901 atgttcaatt tattatgtaa ttaattatag cattgatgaa caaggtgtgc aatgtatttt
    24961 tgcaatatta caataaatga atttaattat attagaatta agtatttcga aaaatgattt
    25021 aatagttgag tcataggatc attagataaa gcgaataatc aattataggg acattgggat
    25081 acaaatcaat gtactagact ctacaaggct atattcaggg acccaacaaa gagaaattgg
    25141 attcccaatt tctagagaca atgcaagttg gggtgggacg acgaaataaa ttttgtgaaa
    25201 atatcatttc tgtcccactc ccagaaccac tacataataa atcattagtg gctctttatc
    25261 atttctgccc cataaccttg tgaattttta aaagaaatgt gtcacaaatc cacgcgacat
    25321 tgataatcgt tatcaactaa tatatgatgt acttgtaata agtattaatt tttaacaaat
    25381 aacaaattta tattatgtat atcatccagt gaaataaata atataagcgg cttaatttag
    25441 attattaatt gaaaggagtt acatatacga tgaacaatca gacaaattgg ataaagatac
    25501 tcagcggatt tgcttctgat agtaaatgga aaattatgtt atcaattttg ttgtctatca
    25561 tcagtgtctt ttctggatta gttccttatt gggcagtatt taaaattatt ttaatgatga
    25621 ttaacaatac atatacgatt aattcgatta tggtttatat ctttattgct ttaatcgctt
    25681 atatttcaca agtgtgttgt tttggagcgt caacgatgct atcacatatt acggcatatg
    25741 aaattttatc tgaaattcgt aaaaaattag ctcaaaaatt aatgcgtctc cctttaggcg
    25801 tagtggaatc taagaaaata ggtgaattaa aaaatatatt tgtcgataag gttgaaacaa
    25861 tagaattacc tttagcacat atgattcctg aagttatagg aaacttactt ttgtcagctg
    25921 ctatcttttt atacataatg ctcattgatt ggcgtatggc tagtgcctta ttagtaacga
    25981 taccaatttc aattttcgct tttaaaaaag ttatgtctgg atttaatgag acatatgctg
    26041 aacaaatgaa atcgaataat tatatgaata gcgcgattgt agagtttatc gaaggcattg
    26101 aagtgattaa aacatttaat caatctcaaa gttcatataa aaaatataaa gatgcagtag
    26161 ataattataa gattcacact ttgaattggt ttaaaaatac atgggggtat atgaatttag
    26221 gagctagtgt attaccttca acatttttag ggattttacc ggtcggcatg tatttgatat
    26281 ctatcaacca attaaactat gcggaatttt tcctatgcat cgtattatct ttaggtgtag
    26341 tagcaccgat taagaatttt actaattatg taaatcattt aaagtctata caatacgcat
    26401 taactgaagt gaatcaaata ttaagtctag aagagttagt attgtcaaca aaatttaaga
    26461 agcctcaaca ttatgaaatt gcttttaata atgttggatt ttcatataac aaagataagg
    26521 acgatctggt ctttaagcat ttatcattta cagtaccaga aaataatttc acagctatcg
    26581 tcggggcatc tggtagcggt aagtcaacca ttgccaagct tatatcacga tactgggatg
    26641 tgacttcagg tgaaattacg attggcggta taaatataaa agatattgaa tcgaaacaac
    26701 ttaacgattt agttggtttt gttggacaag ataacttttt attaaatctt acttttaaag
    26761 aaaatattaa acttggcaat ccagaagcta cggatgaggc agttgaaaaa gctgccaagt
    26821 tagcacaatg tcatgaattt attgaaaagt tgccagatgg atatgatacg aatgttggta
    26881 cagtgggaga taaattgtct ggtggtgaaa aacaacgagt cactattgca agaatgatat
    26941 taaaagatgc gcctatcatt gtattagatg aagcgactgt ttacgttgat ccagacaacg
    27001 aacaaaaaat tcaagaagca ttaaatgtgt tgacgcagga taaaacattg attgttattg
    27061 cacatcggtt atctacgatt caacatgcag atcaaattat tgttttaggt aaacaacaaa
    27121 ttttagaaaa agggtcacat cacttattgc taaaattaaa cggtaattat aagaaaatgt
    27181 gggatacgca catgcataca aaagattggg gaatcaatac tggacataat taaactaatg
    27241 agaaaaggag gtcaaatatg tttcaaatta catttaaaat tttaaactgg ataagaccat
    27301 ataaagcaag aatgatttta ggatttagca tgtcattttt aaatgctatt tttattgcgt
    27361 tacctatctt tttagctgca aaaatattta ataatgtgct atctcataaa cctatttata
    27421 tgaaagatat attgaatgtt gtaattatca tggttttatt agtgattgga cgatttatta
    27481 cagcatattt caaaagcaaa agccatgaga gcatcgctta tgaaatgagt gcgaaagaac
    27541 gtttagatat aggggataaa ttgaaaaatg taaggttagg ttattttaat tcgcatcatt
    27601 caaatgagtt aacaacaata gtaacaacag atttaacctt tttagaaaac tttgctatga
    27661 aaatggtgga cgttgttgtt aatggataca tattaattac agtactcata ttgtctctac
    27721 ttgtagtttc gtggcaagta tcattattag catgcattgg cgtattacta tcattttttg
    27781 cgattcaatt attagaaaga aagagtcgac aaaatgcgcc agcgtatcat aatgtacaaa
    27841 accaattagt ggaaaaggta ttggaggtta ttcgtggtat tcaagtaata aaatcattcg
    27901 cgaaagaaaa tacgagtctt aaaagtttta accaagcagt caatgaaagt aaacgtataa
    27961 atacaaaaat agaaatgcaa tatatcccat tcaatttatt gcatttactt agtttaaaag
    28021 ttgtttcaat aatgattgta ttagttgcat gcttattata tatgaatcat agtattgatt
    28081 tacctaccct tattatgatt tcaatttttt catttgtgat atttgatagt gttgaaaata
    28141 ttaatagtgc tgcacacgta cttgaaatga tagatatgac gatagatgat attgaaaaga
    28201 taaaaaatgc tccagaactg gatgagaatg gaaaaaattt gacgattaaa aatgaaaata
    28261 tcgcttttca aaacgtgaac ttttcatatg atgataaaca agtgataaag aatgtgaatt
    28321 ttgagatacc tacacaaaca tcaacagcaa taattggacc ttcaggaagt gggaaatcta
    28381 cattatgtca cttactcttg cgcttttatg atatcgatga tggaaatatt cgcatcgatg
    28441 gtgttgatat taaagatatg acattaagta cgttaatgtc gaaaattagt gcagtatttc
    28501 aaaaggtgta tttatttaat gatacgattg aaaataacat attgtttggc aatccaggtg
    28561 caacgaaaga agaaattatt cgtgcagcga agcaagcatg ttgccacgac ttcatcatgt
    28621 cattacctga gggatatcaa acaatgctaa atgaaaaagg tagtaattta tctggcggag
    28681 aaaagcaaag gatttctatt gctagggcga tattaaaaga tgcaccaata attattttag
    28741 atgaagcaac tgcaagtatt gaccctgaaa atgaacagct gattcaaacg gcaattaatg
    28801 aattaagtaa aggcaaaaca gtaattacaa ttgcacataa acttgaaact attaaaaatg
    28861 cagatcagat tatagtgctc aatgaaggtg aaataattca aaaaggtagt catgatgaat
    28921 taattcgaaa accaggaatg tatcaagact ttataagaat aaaaagtaaa tcagcaggct
    28981 ggaaattata aataatagtt taatatggaa tcagaatttg gaaataaaaa gggtgataga
    29041 taatcaacag caaaataaat cccctcacta gggcaatagt gaggggattt ggtgtattta
    29101 gatgttcaat ttattatgta actaattata gcattgatga acaaggcgct caacgtaata
    29161 ttacaattaa tggattgttt cacaagatgg taaagctagg atgtcttttt attaagaaat
    29221 aatgcaatta ttaattttaa ctgacatatt ttatcgaatc gctgtttata tctgcccgaa
    29281 ttccttaaga agtcttagtc atttcaatag caaggtcaaa attttgccaa aatttatagt
    29341 tatactgaga agtttaaatt catgttttct tgaaaaacat gcgccttaaa tgtaaactta
    29401 ttaattataa aagtttacat ttggattgag gtggttattt ttatgaggat ttttattaca
    29461 agtacgaata ctgatgtagg caaaacctat gttacaaagc atttatacca tgctttgaaa
    29521 acacgtggtc atcgagtttg tatttttaaa ccatttcaaa ctgaggaacg ccaagacggg
    29581 acgtttccag atttagaagt atttaaaaat gaatgtgatt taagctatga cataacgtca
    29641 ctttatactt ttaagcaacc tgtatcacca caccttgcat ttaaaatgac agatcaaatt
    29701 tttctaaata agcagcgtgt attagataag gtaaaagttt tagataagga atttgatttt
    29761 atcttaattg agggtgctgg gggaattgcc gtaccaatat atgaaggtac agatgatttc
    29821 tacatgacta aagatctaat caatgattgt gcagattgtg tcatcagtgt gttgccatca
    29881 aaattaggtg ctattagcga tgccattgtt caccaagatt atgttaatca gaatgtatcg
    29941 gcgagtaatt ttttaataat gaatcgctat acagacagct atattgaaaa agacaatcaa
    30001 atgacgattg gaaaattaac aaataaaaca gtctatacat ttgaagaaca tgccacgtat
    30061 gaaaatttct cagaagcatt tttaaaacaa ttaataggag ttaaaaatga attacacaca
    30121 acaacttaaa caaaaagact cagaatatgt ttggcatcca tttacacaaa tgggtgtata
    30181 tagcaaagaa gaagcaatca tcattgaaaa aggaaagggt agttaccttt acgatacgaa
    30241 tggcaataaa tatttagatg gttatgcatc gttgtgggtc aatgtgcatg gtcataataa
    30301 caaatactta aataaggtaa ttaaaaagca actcaataaa attgcccatt ctacgctgct
    30361 aggatcatca aatattccgt caatagaact tgcggaaaaa ttaatcgaaa tcacgccaag
    30421 taatctaaga aaagtatttt attctgatac aggcagtgcg tctgttgaaa tcgcaataaa
    30481 gatggcatat cagtattgga aaaatattga tagagaaaaa tatgccaaga aaaacaagtt
    30541 tataacgcta aatcacggtt atcatgggga tacgattggt gcggtaagtg ttggtggtat
    30601 caagaccttt cataaaatat ttaaagactt aatatttgag aatattcaag tagaaagccc
    30661 atctttctat cgcagtaatt acgatactga aaatgaaatg atgacagcta ttttaacgaa
    30721 tatagagcaa attctaattg aaagaaatga tgaaatcgca gggtttatat tggaaccgtt
    30781 gattcaaggt gcgacaggct tgtttgttca tcctaaaggc tttttgaaag aagtcgagaa
    30841 attgtgcaaa aaatacgatg tcttattaat ttgtgatgag gtagcagttg gttttgggag
    30901 aactggaaag atgtttgcat gcaatcatga agatgttcaa ccggatatta tgtgtttagg
    30961 taaggcgatt actggtggct acttaccact tgcagctaca ttgacatcta aaaaaatata
    31021 caatgcattt ttaagtgatt cgcatggtgt gaataccttt ttccatggtc atacatacac
    31081 cggaaatcaa atcgtttgta cggttgcatt agaaaatata agactttatg aaaaacgtaa
    31141 gttattgtca catattgaaa cgacatcatc aacacttgag aaacagttac atgcgctgaa
    31201 gcgtcatcga aatgttggtg atgtaagagg acgaggctta atgtttggag ttgaattagt
    31261 tacagataaa gatagtaaaa cgccgttaga aattgaaaaa gttgaacgta ttgtacgtaa
    31321 ttgtaaagaa aacgggctaa tgattagaaa tttagaaaat gtcattacgt ttgtgccagt
    31381 gttaagtatg tcaaataaag aagtgaaaac gatggtacgt atttttaaaa aggcagtaca
    31441 taacatttta gataggaagt gttaatatga atttggctaa acgcatatta caaggggaac
    31501 aattaacaaa agagactgta ttgaaaattt atgaggatac taatattgat accttagatt
    31561 tattaaatga ggcgtacatt ttaagaaaac attattttgg taaaaaagta aaattaaaca
    31621 tgattttaaa tgctaaaagt ggtatatgtc ctgaaaattg tgggtactgt ggacaatcac
    31681 gagatattaa acaaaaacag cgatatgctt taattccaga ggaacaaatt atcgatggag
    31741 caaaggtggc acatgataat catattggaa catattgtat tgttatgagt ggtagaggac
    31801 cgagcgataa agaagttgat catattagta atactgtaag aacgattaaa tctcaacacc
    31861 cgcaactaaa aatctgtgca tgtttaggat taacgaatga cgaacaagct aagaaactta
    31921 agtcagctgg tgtagacaga tataaccaca atattaatac aagtgaaaat taccatgata
    31981 acgtcgtgac aacgcatagt tataaagata gaacagatac gatagaacta atgaaagcga
    32041 ataatatatc accatgttct ggcgtgattt gtggtatggg agaatctaat caagatattg
    32101 ttgatatggc atttgcttta aaagaaatgg atgccgacag tattccgatt aattttttgc
    32161 atccaatcaa aggcacaaag tttggaagca tggatgattt aacaccaatg aaatgtttaa
    32221 gaatcgtagc attattccga ttaatcaatc ctacgaaaga aattcgtatt gctggaggaa
    32281 gagaggtcaa cttacgttcg ttacagccat tagcattaaa agcggcgaat tcaatatttg
    32341 tcggtgatta tctaattact ggtgggcaac cgaaccaatt agactacgat atgattaatg
    32401 atttaggctt tgaaattgat tatgacactt gtgaaaataa ggaaaacaag aatgaagttt
    32461 caagagcaaa ttgatttatt acaacagaaa gggttatatc ggtcgcttaa atcggtaggg
    32521 tatatagata gacagtatat tgaagtagaa aataagagat gtacgaacta tacatctaat
    32581 gattatttag gattaggtca aatagcgttt gataaggatg atttcgaaag atttatgcgg
    32641 aagtatagtt atcacttatc aagttcaaga ttaattagtg gaagttcgac agcttatgaa
    32701 gaaattgaaa caatgttagc aggttggctc ggatatagtg catgtactat cttaaatagt
    32761 ggttatgatg ctaatttggc gttatttaat attttcaaaa atacaaattg tgtcgtgttt
    32821 tcagatcaag aaaatcatgc gagtattatt gacggtatta agttaagtgg tttagaaaaa
    32881 gtgatatata agcatttaga tattgctgat ttagaaaaaa ggttagagaa ataccctaat
    32941 caaaatatac caaaaataat catatctgat agtgtatttt caacgaatgg cgacgttgtg
    33001 gatattggtc aattagtctc attaaagcat aaatataacg caacactgat actcgatgtt
    33061 tcacatagtt ttggaataga gaattactcg aattatcaag gtgtagatat actcacttct
    33121 agtttatcta aagcatgtgg tgcatacggg ggtgtgattt taagttcaaa tgatgtgaag
    33181 gatatgttaa ttaatcacgg tagaccactc atctactcaa gtagtttgcc aatttataat
    33241 ttgtatttta taaaaagaaa cattgaaaag ttaataaatg ctgatgatag acgcactaaa
    33301 ttaaatagtt tgagtaaata ttttaaccaa aagttaaaag cactcaatgt taattataat
    33361 agttcaaact cgccgattaa gtttattgag tttgatgaca tagaagcggc tgagaacatt
    33421 catcaaacat tattaaagca tcatgtgttt acaagttatt taaggtatcc aactgtgact
    33481 aagccaatgt taagaatatc attgtcgtat tttcatactg aacaagatat agataggttg
    33541 tttgaaattt tgcatcaaga agattgaggt gatcgtcatg tatagtataa aaatgcgttc
    33601 tagcaatcaa gatgttcata ttagtggtgc tgaaacgatt tgtgagtttg acaaaataga
    33661 acagacggta caaaggtttt ataataaagg tttttttcat gaaaatgggc aaccggattt
    33721 tctcaatata aaaatacaaa aaatcatgga accgattcaa caaataaaag cattacaaat
    33781 tattgaggat gataaagcaa atctccaaca tttaacacaa gaatgtggtg tcactgaaca
    33841 agcgctaaat caagggatga catatattaa aaatgaaacg gtttatacag gagctattat
    33901 tctatctgct atatctggta aacgtttaga ttcatttgga caaaggggga ttagggcaac
    33961 acatttttcg tttgaagata taaataataa aggtgatctt aatgaaagag tgactgatgc
    34021 cttagcaatt gctagttgta tcaatgcgca tccgtatgtc aaaggagaac tttgcgtgtc
    34081 cgatgactta acgtatacga caggttattt tgccgctgct aaaattggtt accatcgatt
    34141 atttgatatt aaaccagtta atacgagata tggaggcaga ataatatttg tggacgattg
    34201 tattgattta aatcattaca tatcattttt agaaagcaca ccgaagcaag ttgtttatga
    34261 aacggtatag gggttttagt atgacatcaa aagatattac tcaaattagt gtcattgctg
    34321 cgattttaac cattttggca gttttgaaaa taccgtccat tataccagga ttagattttc
    34381 aattatctgc accggcagca ttattgatat tagctttctt tggaattaaa aagtactttt
    34441 taggtggatt attatctagc ctattattac tagtatttgg cgtatttaat ccaattaatg
    34501 tgattatctc tattatattt agagttatag ctattgcagt tgtttattta ttgaaaataa
    34561 atgtactatc attagtttta gcaagtgtat taggcagttt ggtatctagg ctactattat
    34621 ctattatttt aaatttacct gtgtgggtag tgttgttaaa cgcgattcca ggcgtaatat
    34681 tcactttaat tgtagctatt cctttatatc tcacattgag aaaaagaatg gcagtattac
    34741 taagataata aatcaaaaca cggtcgtcac aattactgtt ggcgaccgtg ttttactagc
    34801 tatttattgt tttcagtttc ttttgtatct aacaatttca ctttgtgatt ttcccaatca
    34861 atttcatatg ttgatttaaa tgttctagtt ttaaagtttt tataatttgc gcctgcccag
    34921 tagaagccat tccaacgaat ttggtataaa tccatttcac gttgataagt tactgtaatt
    34981 ttagattttt tagcgccatc ttgtctgtgt gatagtacgc ttaaaaattc tggattgaag
    35041 ttacttctag ataataatgg catttggtgt tgcgctatga agttttggcc agcgtatgca
    35101 ctgctttgtc tgccagctaa gaagagttca ttaccatatg ttgggtggaa gctatctctt
    35161 ccataaggtc cccaaccatt attcataatt ttatgtgctt caactcccca gccaacattt
    35221 ttataatttg tgttgcgact taatgttgtt ctgtaacttt cttgtttata attaattgtt
    35281 tcagaaaaag ctgtatttcc attaagtcca ccagataaac cattagagat actaatgtca
    35341 ccaccaaatg tatagcctaa agtattttga acttgaaact cttcattttg attttttggt
    35401 gcataatcaa cgacgtttac tgaatcatta gattgtgagc ttatagatac attgtattta
    35461 gctccccaat ataattttga aaagtcatag tcattaggat taggtttcac aaagcctgag
    35521 ttaatattcc cagtagcttt aagtactaaa gtatctttat cataactttt atctttgatg
    35581 aaattaaatg ttaaaatctg tgaaatttta aatttatcag aatctgctgt ggctgttgtt
    35641 ttgtataaag taactttgtc atcgactttt tttacgctga ctggtgttat tttaccttca
    35701 gcattagcag taccagaaag taataataat gccatagatg tagcaacgga tgatttgact
    35761 aatttattca ttttcatatc aattctgtcc tttcaccttg atttcatgag tcttccaatt
    35821 gacctcgtat ttcacagtat agtttctatt tacaaatgca ttatggactc tatgtccgtc
    35881 taaataactg ttgccataat gcgttgatct tttaatggca tgagtgacat ccatgtttct
    35941 tccgtaagta atttcaaatt cgcttgtatc gcttgaacct ttttcatgag atactgtggc
    36001 gataaatgaa gggttaaatc cactttgtac aagaggtggt aactcactgt ctggaacgaa
    36061 ataatctcta ggatctttac tatgaggttt gtagcctaca aataaatcgc tatcaaaggc
    36121 tgatttttga cctgattcag tggcgaatga attcgctttg acgccccata aaacactttt
    36181 tgagttttgt tgttctactt cacttacata attttgttgt gtatagctaa tcgatttaga
    36241 atagttaaat gatccattac caccgagtga tggggctgat tggaaattac caccgatatt
    36301 gtatcctaat gtctgactca cgtttgtaga ttcaatttta tttttaggta aataattaat
    36361 taaagaaaca tatttatcat ttgtttttaa accaatatta tattggaatg gccatcgcat
    36421 agctttaaca tgattagttt ttttatagtt gtaatatgtt gttctagagc taatgaatcc
    36481 ttgcatcttt aatatcaaag catctttgtt atatttttta tcctttacaa aatcaaattg
    36541 aatattttga gtcacgcccc atttattact tgttttatct tctgtccttt tgataatttc
    36601 tatatcgctt cctttaccga tgtcttcagt atcgttagca gctttagcat tttctaataa
    36661 cggattggca agaggggcaa gtaagctcac agataaagtt gtagttaata ttttattttt
    36721 aaacataagt ttcactttat ttctataatt ttatttaact taattaaata ataatatatt
    36781 ttgggcgtta ttataattaa ctgttcatta acaattgacc attatcaact ttttttacaa
    36841 ttaagttaaa aatgccataa ttccgttcga tttaaccttt tttgtttgga atgctaaaag
    36901 ctaattttgt gagaaatata gttaataaaa ccttggtatt gttgacagtt gtaaacaatt
    36961 taaggtaacc gattgcacga atgtcttgct ttctttttta attaatgttt gtaaaataca
    37021 aattgtgctt tttacgattt ttgatgaatc catctaaaaa ttaattgttt attaacttga
    37081 taatatttat ttaagctttc aagttaaatt gtttcatttg tattatttgt gtatctacta
    37141 tggttcgata ctttattctt ataaaaatca gttgttatta ttaaatttaa tgtacgataa
    37201 aataatttaa tgttataagg ctgattcaat ttgttaaaaa atcctgagac aagtatgaag
    37261 tgtcccagga ttttttaaga tgattgaact gtttacttag gtgtgatgct tttaattttt
    37321 acttcatgtg ttttccagtt cacttcatat ttaactgtaa cgtttcggtt tttaaaagca
    37381 tcatgttttc tatcaacggc taaacgatgt cttgtcacgt aagcatatgt agcatccatg
    37441 tttctgccgt aagtgatttc aaactcgctt ttatcacctt tacctctttc gtgtgacaat
    37501 gttgtaataa atgatggatt aaagccactt tgaattaaag gaggtagttg attatctggg
    37561 acgaaatagt ctcgtgctgc tggaccagtt gggtcttgtg caaataagta ttgatcatat
    37621 gcagatactt gaccattcgg tgtaacgaat gaatttgctt tcactcccca tttaacacct
    37681 ttagagttct gactttctac ttcagtaaca tagttttttt gattataact aattgtttta
    37741 gagtagttga atgagccact gcctccgatt gatggcgctg attggaagtt tccgccgata
    37801 ttatagccta atttctgact aacatctgct gaatcaattt tatttttagg aagataatta
    37861 attaaatcaa cattagagtc tttcgttttc aaactgatat tatattgaaa tggccatatc
    37921 attcttttaa tatatggata tttttttaag tctgaatatg ttgttctaga gctaatgaag
    37981 ccttgcatct taacaactag ggcatctttg ttatattttt tatcttttac aaaatcaaat
    38041 tgaatgtttt gagttatagc taatcgtttg ctagtaatgt cttgtgttct tttgatgatt
    38101 tctgcacctt ggccgatatc ttctatctta ttttctgctt tagaaatttc tataaatgga
    38161 ttggctaaag gggctattaa accaactgct aaagttgctg ttaatatttt atttttaatc
    38221 atagaaatca ctttctttct atttaatttt aagttcatat atataataat ccatttgtaa
    38281 gcgttttcga attaactgtt cgttaaaagt taattactat caacatgtat tgaagagaca
    38341 tttttagcat tgttaaatga tgtttttgca ttatgttatg tttaaaagat ttattggtcg
    38401 caggcgttta tatagctaca taactatgct ttgcaagtct tattttattg aattgttgtt
    38461 taattatggt gcttgtagtg gaaaaggaat ggattgcgca atagtttgtg aaaatatgaa
    38521 gttaataaag ttgagctgat tcaactaaat gatgaactat tcgttaacga tgtaaggatt
    38581 atttaacaag ttgcaggtaa aaaagtgggc tgacaaatag cgaagaagca tttaattgat
    38641 ggaaaatgaa aaaagaaccg tagattcgat tgagaatcaa cggttctttt taatcacggt
    38701 ttgttacgaa aattatttaa aaaatgatgt aaaactagag aagatatttt tgattgatta
    38761 tttccagaat gataataaat tacttaatgt atttttgatt gatgatgcag tttcttgagc
    38821 tttaatagct gtttttacta atgttgggtt ttggtagaaa gtatataata ctttaccagt
    38881 attgattgac tcagttacat agtttcttac ttgtgcatat gatttagcta agtagccgtt
    38941 tttatcatca actttcaatg gttggatgta agatttagaa ccactaccta gtactgttaa
    39001 tactgtttga tcaatcgcac ctttgtattt atagtacgta ttatagtagt actttgctgt
    39061 atcatatttt tctttatagc ttttataagt atctgttaaa tacttgtaac catagttaaa
    39121 tgaatctttt aatgattggt agtaacctaa taatttagat tgaggaacct caacttttgg
    39181 tgattgaatt tgagggactt caacttttgg tgattctact ttaggttttt caatttgtgg
    39241 tgattgaatt tgaggagcct caacttttgg cgccactttc ttttcagcat ctttttgagc
    39301 aactaatgcg tctaattgtt tctgtaaatg ctcttttaca tccataggtg ctttgttaac
    39361 ttcacgttgt gctaaacgtc tgttttcaat tgaatctttt tcatttaatt ttgagattgc
    39421 atcatttgct gatttcacac gctcatcatg acgaacgatt gctttttcaa ttgaaacttt
    39481 ttctacaaag cggttgtttt tatcacgtaa atctttaggt aattttgagt tagcttcata
    39541 tgctgctgat ttatcgtgtg gtgctttgtt atcttggaaa tctttaattg ctttatccgc
    39601 attttcaata ttttgacgtt ctttagcttt agaagattgt actgattcta cccaaacttg
    39661 ttggcttcta tcagggtttt ctttaatttg tgcaatgtaa ttattttttt cttgttcagt
    39721 taagttatca tttttaagaa cattgtaaaa agcgttttgt tgtgcaacac gtcggtctgg
    39781 gttcttgctg tctttaagtg attcagagaa tacttcttgt gcacgttctg ggtgttcgcg
    39841 taatgttttg atgtattggt tacgttgttc ttctgtgata ccttttagat gtaatacttg
    39901 ataaaaagct ttttgttgat ctgttacgta gttgttttga gttgtttggt gcttagttga
    39961 agtttgttgc gtgttttcac tcgcttttgc ttccccattt gaaatcattg tagctaacgt
    40021 aattgttgct gccccaacta gcaacttcga gatatattta tttttcatgt gtattccctt
    40081 tctttttaca tattaaattt attgtatgct tttagaattt atcacatgag aatacaaatg
    40141 gaaattaact aattattaac taatgttaac taatagatta actagtgtta actatgtaaa
    40201 aaagcgttta attcagaaca ttcagcgtat tttaagtaag gaagtactat atggagaaat
    40261 gcaatagttt gctaataaat agaggagaaa attgtctttc gaagagagtt tggtagagta
    40321 gaaatctcat ttaatttgtc attgatgcac tttttcttct atataattag ttcacgacat
    40381 tagatagctt cgcaactcat gcatagggtt ctaacataga aactttcgag gcgaaagtta
    40441 acaaacaggg agagttgggt gcctttctaa caaataaatt tgaagaagac ggaggaacgc
    40501 agttggacgt aaaacgtcaa ctgcataagc ctttctagct cataaaattc gcagaaaata
    40561 aaggaacaca ttagatagct tcgctactaa tgcatagggt cccaacatag aaactttcga
    40621 ggcgaaagtt aacaaacagg gagagttggg cgcctttcta gctcataaaa
//
LOCUS       DALAGD010000006        39231 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.006, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000006 DALAGD010000000
VERSION     DALAGD010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 39231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39231
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.006"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            39..992
                     /locus_tag="O2701_000240"
     CDS             39..992
                     /locus_tag="O2701_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292156.1"
                     /translation="MKKTLGCLLLIMLLVVAGCSFGGNHKLSSKKSEESKQETVKKES
                     EEEKDPDLEKYEEIEKKMKGIKDAPSLDKLDPLMTEKSFTNSKGIQGWKDYKELMGKV
                     ELADYRFTKDSKGSSIKDVDAFFKGKKGIKRKVIETHDDVKQVDYWYVDPDGKKIGNS
                     NTPVFYAEIMTKYKDGKLVYASVEPGSYVIHKDDAIKYDDYSKLKKLSQLTKLDHPKP
                     VPYSVAQIKSFGVPLTSVSFMTHGSKDTKDEVLPALAYFTFSPKNYEDKSNPDPKVLN
                     LVHMDFLNASSDFGNAHFVVLSKYIKEYESNYETASDDSLK"
     gene            complement(1155..1358)
                     /locus_tag="O2701_000241"
     CDS             complement(1155..1358)
                     /locus_tag="O2701_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HCZ4292157.1"
                     /translation="MQVNKVIYILLALFLGSFGIHKFYAGKNMQGILHLIFCWTGIPH
                     ILAIISAVITVFKPADEQGNVTL"
     gene            1657..2145
                     /locus_tag="O2701_000242"
     CDS             1657..2145
                     /locus_tag="O2701_000242"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCZ4292158.1"
                     /translation="MAIKKLVPASHPILTKKAQAVKTFDDSLKRLLQDLEDTMYAQEA
                     AGLCAPQINQSLQVAIIDMEMEGLLQLVNPKIISQSNETITDLEGSITLPDVYGEVTR
                     SKMIVVESYDVNGNKVELTAHEDVARMILHIIDQMNGIPFTERADRILTDKEVEAYFI
                     ND"
     gene            2138..3073
                     /locus_tag="O2701_000243"
     CDS             2138..3073
                     /locus_tag="O2701_000243"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCZ4292159.1"
                     /translation="MTKIIFMGTPDFSTTVLEMLIAEHDVIAVVTQPDRPVGRKRVMT
                     PPPVKKVAMKYDLPVYQPEKLSGSEELEQLLQLDVDLIVTAAFGQLLPESLLALPNLG
                     AINVHASLLPKYRGGAPIHQAIIDGEQETGITIMYMVKKLDAGNIISQQAIKIEENDN
                     VGTMHDKLSVLGADLLKETLPSIIEGTNESVPQDDTQATFASNIRREDERISWNKPGR
                     QVFNQIRGLSPWPVAYTTMDDTNLKIYDAELVETNKINEPGTIIETTKKAIIVATNDN
                     EAVAIKDMQLAGKKRMLAANYLSGAQNTLVGKKLI"
     gene            3070..4377
                     /gene="rsmB"
                     /locus_tag="O2701_000244"
     CDS             3070..4377
                     /gene="rsmB"
                     /locus_tag="O2701_000244"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCZ4292160.1"
                     /translation="MIENVRSLAFDTIQDILNEGAYSNLRINEVLSENELNAMDKALF
                     TEIVYGTVKRKYTLDFYLKPFVKTKIKAWVRQLLWMSIYQYVYLDKVPNHAIINEAVE
                     IAKERGGYHNGNVVNGILRTMMRSDLPDFNEIADPKKRMAIEYSMPKWIIDHWATHYG
                     LEETETILQSFLETTSTTVRANLTRASLDDIIEKLQDEGYDVEKDHDLPYCLHIGGQP
                     IIHSRSFKDGFVSIQDKSSMFVAHIMNVDRHDHVLDACSAPGGKACHIAEVLMPEGQV
                     DASDIHDHKIDLINFNIKKLRLTNIKAFQHDATKPYDKTYDKILVDAPCSGLGVMRHK
                     PEIKYTQSKQHIESLVELQLEILENVKNNVKIGGEIIYSTCTIEQLENENVIYTFLKN
                     NKNFEFEPFQHPITGELVKTLQIMPQDFNSDGFFITKIKRKDN"
     gene            4380..5474
                     /gene="rlmN"
                     /locus_tag="O2701_000245"
     CDS             4380..5474
                     /gene="rlmN"
                     /locus_tag="O2701_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002435140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCZ4292161.1"
                     /translation="MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAK
                     QIFEWLYQKRVDSIDEMTNLSKDLRQLLKDNFTVTTLTTVVKQESKDGTIKFLFELQD
                     GYTIETVLMRHDYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKA
                     LDATEERVSQIVIMGIGEPFENYDEMMDFLRIVNDDNSLNIGARHITVSTSGIIPRIY
                     DFADEDIQINFAVSLHAAKDEVRSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYG
                     LFGGVNDQLEHARELAHLIKGLNCHVNLIPVNHVPERNYVKTAKNDIFKFEKELKRLG
                     INATIRREQGSDIDAACGQLRAKERQVETR"
     gene            5481..6224
                     /locus_tag="O2701_000246"
     CDS             5481..6224
                     /locus_tag="O2701_000246"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase Stp1"
                     /protein_id="HCZ4292162.1"
                     /translation="MLEAQFFTDTGQHRDKNEDAGGIFYNQTNQQLLVLCDGMGGHKA
                     GEVASKFVTDELKSRFEAENLIEQHQAENWLRNNIKDINFQLYHYAQENAEYKGMGTT
                     CVCALVFEKSVVIANVGDSRAYVINSRQIEQITSDHSFVNHLVLTGQITPEEAFTHPQ
                     RNIITKVMGTDKRVSPDLFIKRLNFYDYLLLNSDGLTDYVKDNEIKRLLVKEGTIEDH
                     GDQLMQLALDNHSKDNVTFILAAIEGDKV"
     gene            6221..8215
                     /gene="pknB"
                     /locus_tag="O2701_000247"
     CDS             6221..8215
                     /gene="pknB"
                     /locus_tag="O2701_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase Stk1"
                     /protein_id="HCZ4292163.1"
                     /translation="MIGKIINERYKIVDKLGGGGMSTVYLAEDTILNIKVAIKAIFIP
                     PREKEETLKRFEREVHNSSQLSHQNIVSMIDVDEEDDCYYLVMEYIEGPTLSEYIESH
                     GPLSVDTAINFTNQILDGIKHAHDMRIVHRDIKPQNILIDSNKTLKIFDFGIAKALSE
                     TSLTQTNHVLGTVQYFSPEQAKGEATDECTDIYSIGIVLYEMLVGEPPFNGETAVSIA
                     IKHIQDSVPNVTTDVRKDIPQSLSNVILRATEKDKANRYKTIQEMKDDLSSVLHENRA
                     NEDVYELDKMKTIAVPLKKEDLAKHISEHKSNQPKRETTQVPIVNGPAHHQQFQKPEG
                     TVYEPKPKKKSTRKIVLLSLIFSLLMIALVSFVAMAMFGNKYEETPDVIGKSVKEAEQ
                     IFNKNNLKLGKISRSYSDKYPENEIIKTTPNTGERVERGDSVDVVISKGPEKVKMPNV
                     IGLPKEEALQKLKSLGLKDVTIEKVYNNQAPKGYIANQSVTANTEIAIHDSNIKLYES
                     LGIKQVYVEDFEHKSFSKAKKALEEKGFKVESKEEYSDDIDEGDVISQSPKGKSVDEG
                     STISFVVSKGKKSDSSDVKTTTESVDVPYTGKNDKSQKVKVYIKDKDNDGSTEKGSFD
                     ITSDQRIDIPLRIEKGKTASYIVKVDGKTVAEKEVSYDDV"
     gene            8443..9318
                     /gene="rsgA"
                     /locus_tag="O2701_000248"
     CDS             8443..9318
                     /gene="rsgA"
                     /locus_tag="O2701_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HCZ4292164.1"
                     /translation="MKTGRIVKSISGVYQVDVNGERFNTKPRGLFRKKKFSPVVGDIV
                     EFEVQNINEGYIHQVFERENELKRPPVSNIDTLVIVMSAVEPNFSTQLLDRFLVIAHS
                     YQLNARILVTKKDKTPIEKQFEINELLKIYENIGYETEFIGNDDDRKKIVEAWPAGLI
                     VLSGQSGVGKSTFLNHYRPELNLETNDISKSLNRGKHTTRHVELFERQNGYIADTPGF
                     SALDFDHIDKDEIKDYFLELNRYGETCKFRNCNHIKEPNCNVKHQLEIGNIAQFRYDH
                     YLQLFNEISNRKVRY"
     gene            9319..9963
                     /locus_tag="O2701_000249"
     CDS             9319..9963
                     /locus_tag="O2701_000249"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCZ4292165.1"
                     /translation="MTKLYPSLLSVDFLDLQHELKRLEEAGVDGVHFDVMDGQFVPNI
                     SIGLPILDAVRKGTTLPIDVHLMIENPEKYIASFAEHGADMISIHVESTPHIHRAIQM
                     IKHLDKKAGVVINPGTPISQIEPILDIVDYVLVMTVNPGFGGQSFIDQCVEKIAGLNA
                     IKMERQLNFDIEVDGGVNTDTAKVCVENGATMLVTGSFFFKQEDYKKVTQALKG"
     gene            9970..10611
                     /locus_tag="O2701_000250"
     CDS             9970..10611
                     /locus_tag="O2701_000250"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HCZ4292166.1"
                     /translation="MHINLLCSDRHLPQDIWAKSNEGKWGGVDRGALILLKHQIIPFF
                     SVGDFDSVSKEERQLLTEQLQIKPVQAEKADTDLALAVDKAVALGFDSITIYGATGGR
                     LDHFFGAIQLLLKKAYYKHDVHIEVIDQQNKIELLPKGQHTVEKDKSYPYISFIPMTD
                     DVELSLAGFKYNLARQMLNIGSTLTISNEIESLQAKVTVHDGLILQIRSTDLN"
     gene            complement(10992..11180)
                     /locus_tag="O2701_000251"
     CDS             complement(10992..11180)
                     /locus_tag="O2701_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014614133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCZ4292167.1"
                     /translation="MGKQCFVTGRKASTGNRRSHALNSTKRRWNANLQKVRILVDGKP
                     KKVWVSARALKSGKVTRV"
     gene            11623..11997
                     /locus_tag="O2701_000252"
     CDS             11623..11997
                     /locus_tag="O2701_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCZ4292168.1"
                     /translation="MTLEISNDYGKIDISNEVIASVVGGKAVECYGIVGMASRQQVRD
                     GIAEILGHENYAKGIKVTENNGVVDIDMYIIVSYGVKISEVANNVQSTVKYTLEKSLN
                     VSVNSINIYVQGVRVNNTGKKA"
     gene            12012..13658
                     /gene="fakA"
                     /locus_tag="O2701_000253"
     CDS             12012..13658
                     /gene="fakA"
                     /locus_tag="O2701_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase catalytic subunit FakA"
                     /protein_id="HCZ4292169.1"
                     /translation="MISKINGKLFADMIIQGAQNLSNNADLVDSLNVYPVPDGDTGTN
                     MNLTMTSGREEVENNLSKNIGELGKTFSKGLLMGARGNSGVILSQLFRGFCKNIESES
                     EINSKLLAESFQAGVETAYKAVMKPVEGTILTVAKDAAQAAIEKANNTEDCIELMEYI
                     IVKANESLENTPNLLAVLKEVGVVDSGGKGLLCVYEGFLKALKGEKVEAKVAKIDKDE
                     FVHDEHDFHGVINTEDIIYGYCTEMMVRFGKNKKAFDEQEFRQDMSQFGDSLLVINDE
                     EIVKVHVHTEYPGKVFNYGQQYGELIKLKVENMREQHREVIRKEQHTAKPKMETVETA
                     IITISMGEGISEIFKSMGATHIISGGQTMNPSTEDIVKVIEQSKCKRAIILPNNKNIL
                     MASEQAASIVDAEAVVIPTKSIPQGISALFQYDVDATLEENKAQMADSVNNVKSGSLT
                     YAVRDTKIDGVEIKKDAFMGLIEDKIVSSQSDQLTTVTELLNEMLAEDSEILTVIIGQ
                     DAEQAVTDNMINWIEEQYPDVEVEVHEGGQPIYQYFFSVE"
     gene            13848..15908
                     /gene="recG"
                     /locus_tag="O2701_000254"
     CDS             13848..15908
                     /gene="recG"
                     /locus_tag="O2701_000254"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCZ4292170.1"
                     /translation="MAKVNLIESPYSLLQLKGIGPKKIEVLQQLNIHTVEDLVLYLPT
                     RYEDNTVIDLNQAEDQSNVTIEGQVYTAPVVAFFGRNKSKLTVHLMVNNIAVKCIFFN
                     QPYLKKKIELNQTITVKGKWNRVKQEITGNRVFFNSQGTQTQENADVQLEPVYRIKEG
                     IKQKQIRDQIRQALNDVTIHEWLTDELREKYKLETLDFTLNTLHHPKSKEDLLRARRT
                     YAFTELFLFELRMQWLNRLEKSSDEAIEIDYDIDQVKSFIDRLPFELTEAQKSSVNEI
                     FRDLKAPIRMHRLLQGDVGSGKTVVAAICMYALKTAGYQSALMVPTEILAEQHAESLM
                     ALFGDSMNVALLTGSVKGKKRKILLEQLENGTIDCLIGTHALIQDDVIFHNVGLVITD
                     EQHRFGVNQRQLLREKGAMTNVLFMTATPIPRTLAISVFGEMDVSSIKQLPKGRKPII
                     TTWAKHEQYDKVLMQMTSELKKGRQAYVICPLIESSEHLEDVQNVVALYESLQQYYGV
                     SRVGLLHGKLSADEKDEVMQKFSNHEINVLVSTTVVEVGVNVPNATFMMIYDADRFGL
                     STLHQLRGRVGRSDQQSYCVLIASPKTETGIERMTIMTQTTDGFELSERDLEMRGPGD
                     FFGVKQSGLPDFLVANLVEDYRMLEVARDEAAELIQSGVFFENTYQHLRHFVEENLLH
                     RSFD"
     gene            16111..16683
                     /gene="fapR"
                     /locus_tag="O2701_000255"
     CDS             16111..16683
                     /gene="fapR"
                     /locus_tag="O2701_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HCZ4292171.1"
                     /translation="MRGETLKLKKDKRREAIRQQIDSNPFITDHELSDLFQVSIQTIR
                     LDRTYLNIPELRKRIKLVAEKNYDQISSIEEQEFIGDLIQVNPNVKAQSILDITSDSV
                     FHKTGIARGHVLFAQANSLCVALIKQPTVLTHESSIQFIEKVKLNDTVRAEARVVNQT
                     AKHYYVEVKSYVKHTLVFKGNFKMFYDKRG"
     gene            16688..17674
                     /gene="plsX"
                     /locus_tag="O2701_000256"
     CDS             16688..17674
                     /gene="plsX"
                     /locus_tag="O2701_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCZ4292172.1"
                     /translation="MVKLAIDMMGGDNAPDIVLEAVQKAVEDFKDLEIILFGDEKKYN
                     LNHERIEFRHCSEKIEMEDEPVRAIKRKKDSSMVKMAEAVKSGEADGCVSAGNTGALM
                     SAGLFIVGRIKGVARPALVVTLPTIDGKGFVFLDVGANADAKPEHLLQYAQLGDIYAQ
                     KIRGIDNPKISLLNIGTEPAKGNSLTKKSYELLNHDHSLNFVGNIEAKTLMDGDTDVV
                     VTDGYTGNMVLKNLEGTAKSIGKMLKDTIMSSTKNKLAGAILKKDLAEFAKKMDYSEY
                     GGSVLLGLEGTVVKAHGSSNAKAFYSAIRQAKIAGEQNIVQTMKETVGESNE"
     gene            17667..18593
                     /gene="fabD"
                     /locus_tag="O2701_000257"
     CDS             17667..18593
                     /gene="fabD"
                     /locus_tag="O2701_000257"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCZ4292173.1"
                     /translation="MSKTAIIFPGQGAQKVGMAQDLFNNNDQATEILTSAANTLDFDI
                     LETMFTDEEGKLGETENTQPALLTHSSALLAALKNLNPDFTMGHSLGEYSSLVAADVL
                     SFEDAVKIVRKRGQLMAQAFPTGVGSMAAVLGLDFDKVDEICKSLSSDDKIIEPANIN
                     CPGQIVVSGHKALIDELVEKGKSLGAKRVMPLAVSGPFHSSLMKVIEEDFSSYINQFE
                     WRDAKFPVVQNVNAQGETDKEVIKSNMVKQLYSPVQFINSTEWLIDQGVDHFIEIGPG
                     KVLSGLIKKINRDVKLTSIQTLEDVKGWNEND"
     gene            18586..19320
                     /gene="fabG"
                     /locus_tag="O2701_000258"
     CDS             18586..19320
                     /gene="fabG"
                     /locus_tag="O2701_000258"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HCZ4292174.1"
                     /translation="MTKSALVTGASRGIGRSIALQLAEEGYNVAVNYAGSKEKAEAVV
                     EEIKAKGVDSFAIQANVADADEVKAMIKEVVSQFGSLDVLVNNAGITRDNLLMRMKEQ
                     EWDDVIDTNLKGVFNCIQKATPQMLRQRSGAIINLSSVVGAVGNPGQANYVATKAGVI
                     GLTKSAARELASRGITVNAVAPGFIVSDMTDALSDELKEQMLTQIPLARFGQDTDIAN
                     TVAFLASDKAKYITGQTIHVNGGMYM"
     gene            19563..19796
                     /locus_tag="O2701_000259"
     CDS             19563..19796
                     /locus_tag="O2701_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002510960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCZ4292175.1"
                     /translation="MENFDKVKDIIVDRLGVDADKVTEDASFKDDLGADSLDIAELVM
                     ELEDEFGTEIPDEEAEKINTVGDAVKFINSLEK"
     gene            19912..20643
                     /locus_tag="O2701_000260"
     CDS             19912..20643
                     /locus_tag="O2701_000260"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCZ4292176.1"
                     /translation="MSKQKKSEIVNRFRKRFDTKMTELGFTYQNIDLYQQAFSHSSFI
                     NDFNMNRLDHNERLEFLGDAVLELTVSRYLFDKHPNLPEGNLTKMRATIVCEPSLVIF
                     ANKIGLNEMILLGKGEEKTGGRTRPSLISDAFEAFIGALYLDQGLDIVWKFAEKVIFP
                     HVEQNELLGVVDFKTQFQEYVHQQNKGDVTYNLIKEEGPAHHRLFTSEVILQGEAIAE
                     GKGKTKKESEQRAAESAYKQLKQIK"
     gene            20790..24356
                     /gene="smc"
                     /locus_tag="O2701_000261"
     CDS             20790..24356
                     /gene="smc"
                     /locus_tag="O2701_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HCZ4292177.1"
                     /translation="MVYLKSIDAIGFKSFADQTNVQFDKGVTAIVGPNGSGKSNITDA
                     IKWVLGEQSAKSLRGSKMEDIIFSGAEHRKAQNYAEVQLRLDNHSKKLSVDENEVIVT
                     RRLYRSGESEYYINNDRARLKDIADLFLDSGLGKEAYSIISQGRVDEILNAKPIDRRQ
                     IIEESAGVLKYKKRKAESLNKLDQTEDNLTRVEDILYDLEGRVEPLKEEAAIAKEYKT
                     LSHQMKHSDIVVTVHDIDQYTNDNRQLDQRLNDLQGQQANKEADKQRLSQQIQQYKGK
                     RHQLDNDVESLNYQLVKATEAFEKYTGQLNVLEERKKNQSETNARYEEEQENLMELLE
                     NISNEISEAQDTYKSLKSKQKELNAVIRELEEQLYVSDEAHDEKLEEIKNEYYTLMSE
                     QSDVNNDIRFLKHTIEENEAKKSRLDSRLVEVFEQLKDIQGQIKTTKKEYQQTNKELS
                     AVDKEIKNIEKDLTDTKKAQNEYEEKLYQAYRYTEKMKTRIDSLATQEEEYTYFFNGV
                     KHILKAKNKELKGIYGAVAEIIDVPSKLTQAIETALGASLQHVIVDSEKDGRQAIQFL
                     KERNLGRATFLPLNVIQSRVVATDIKSIAKEANGFISIASEAVKVAPEYQNIIGNLLG
                     NTIIVDHLKHANELARAIKYRTRIVTLEGDIVNPGGSMTGGGARKSKSILSQKDELTT
                     MRHQLEDYLRQTESFEQQFKELKIKSDQLSELYFEKSQKHNTLKEQVHHFEMELDRLT
                     TQETQIKNDHEEFEFEKNDGYTSDKSRQTLSEKETYLESIKASLKRLEDEIERYTKLS
                     KEGKESVTKTQQTLHQKQSDLAVVKERIKTQQQTIDRLNNQNQQTKHQLKDVKEKIAF
                     FNSDEVMGEQAFQNIKDQINGQQETRTRLSDELDKLKQQRIELNEQIDAQEAKLQVCH
                     QDILAIENHYQDIKAEQSKLDVLIHHAIDHLNDEYQLTVERAKSEYTSDESIDALRKK
                     VKLMKMSIDELGPVNLNAIEQFEELNERYTFLSEQRTDLRKAKETLEQIISEMDQEVT
                     ERFKETFHAIQGHFTAVFKQLFGGGDAELQLTEADYLTAGIDIVVQPPGKKLQHLSLL
                     SGGERALTAIALLFAILKVRSAPFVILDEVEAALDEANVIRYAKYLNELSDETQFIVI
                     THRKGTMEFADRLYGVTMQESGVTKLVSVNLNTIDDVLKEEQ"
     gene            24356..25606
                     /gene="ftsY"
                     /locus_tag="O2701_000262"
     CDS             24356..25606
                     /gene="ftsY"
                     /locus_tag="O2701_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCZ4292178.1"
                     /translation="MSFFKRLKDKFATNKENEEVKSLTEEQGQDKLEDTHSEGSTQDA
                     NDLAENAEVKKKPRKLSEADFDDDGLISIEDFEEIEAQKMGAKFKAGLEKSRQNFQEQ
                     LNNLIARYRKVDEDFFEALEEMLITADVGFNTVMTLTEELRMEAQRRNIQDTEDLREV
                     IVEKIVEIYHQEDDNSEAMNLEDGRLNVILMVGVNGVGKTTTIGKLAYRYKMEGKKVM
                     LAAGDTFRAGAIDQLKVWGERVGVDVISQSEGSDPAAVMYDAINAAKNKGVDILICDT
                     AGRLQNKTNLMQELEKVKRVINRAVPDAPHEALLCLDATTGQNALSQARNFKEVTNVT
                     GIVLTKLDGTAKGGIVLAIRNELHIPVKYVGLGEQLDDLQPFNPESYVYGLFADMIEQ
                     NEEITTVENDQIVTEEKDDNHGSK"
     gene            25593..25925
                     /locus_tag="O2701_000263"
     CDS             25593..25925
                     /locus_tag="O2701_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HCZ4292179.1"
                     /translation="MGQNDLVKTLRMNYLFDFYQSLLTNKQRNYLELFYLEDYSLSEI
                     ADTFNVSRQAVYDNIRRTGDLVEDYEKKLELYQKFEQRREIYDEMKQHLSNPEQIQRY
                     IQQLEDLE"
     gene            25951..27318
                     /gene="ffh"
                     /locus_tag="O2701_000264"
     CDS             25951..27318
                     /gene="ffh"
                     /locus_tag="O2701_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002457378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCZ4292180.1"
                     /translation="MAFEGLSERLQATMQKMRGKGKLTEADIKIMMREVRLALLEADV
                     NFKVVKEFIKTVSERALGSDVMQSLTPGQQVIKIVQDELTQLMGGENTSINMSNKPPT
                     VVMMVGLQGAGKTTTAGKLALLMRKKYNKKPMLVAADIYRPAAINQLQTVGKQIDIPV
                     YSEGDQVKPQQIVTNALKHAKEEHLDFVIIDTAGRLHIDEALMNELKEVKDIAKPNEI
                     MLVVDSMTGQDAVNVAESFDDQLDVTGVTLTKLDGDTRGGAALSIRSVTQKPIKFVGM
                     SEKLDGLELFHPERMASRILGMGDVLSLIEKAQQDVDQEKAKDLEKKMRESSFTLDDF
                     LEQLDQVKNLGPLDDIMKMIPGMNKMKGLDKLNMSEKQIDHIKAIIQSMTPAERNNPD
                     TLNVSRKKRIAKGSGRSLQEVNRLMKQFNDMKKMMKQFTGGGKGKKGKRNQMQNMLKG
                     MNLPF"
     gene            27753..28028
                     /gene="rpsP"
                     /locus_tag="O2701_000265"
     CDS             27753..28028
                     /gene="rpsP"
                     /locus_tag="O2701_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCZ4292181.1"
                     /translation="MAVKIRLTRLGSKRNPFYRIVVADARSPRDGRIIEQIGTYNPTS
                     ANAPEIKVDEALALKWLNDGAKPTDTVHNILSKEGIMKKFDEQKKAK"
     gene            28216..28719
                     /gene="rimM"
                     /locus_tag="O2701_000266"
     CDS             28216..28719
                     /gene="rimM"
                     /locus_tag="O2701_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCZ4292182.1"
                     /translation="MRVEVGQIVNTHGIKGEIKVKSNSDFTDVRFQPGQVLTVVHNNN
                     DLEYTVKSHRVHKGLHMLTFEGINNINDIEHLKGSSIYQERDHEDIVLEENEFYYSDI
                     IGCTVFDDQETPIGRVINIFETGANDVWVIKGSKEYLIPYIADVVKEVDVENKKIIIT
                     PMEGLLD"
     gene            28719..29456
                     /gene="trmD"
                     /locus_tag="O2701_000267"
     CDS             28719..29456
                     /gene="trmD"
                     /locus_tag="O2701_000267"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCZ4292183.1"
                     /translation="MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAIN
                     KHNQVDDYPYGGGQGMVLKPEPVFNAMEDLDVTEQTRVILMCPQGEPFSHQKAVELSK
                     ADHIVFICGHYEGYDERIRTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGN
                     EQSHQDDSFSDGLLEFPQYTRPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKR
                     PDLIEKYPLTNADKQILERYKIGLKKG"
     gene            29559..29909
                     /gene="rplS"
                     /locus_tag="O2701_000268"
     CDS             29559..29909
                     /gene="rplS"
                     /locus_tag="O2701_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCZ4292184.1"
                     /translation="MTNHKLIEAVTKSQLRTDLPSFRPGDTLRVHVRIIEGTRERIQV
                     FEGVVIKRRGGGVSETFTVRKISSGVGVERTFPLHTPKIEKIEVKRRGKVRRAKLYYL
                     RSLRGKAARIQEIR"
     gene            complement(30153..32759)
                     /gene="yfhO"
                     /locus_tag="O2701_000269"
     CDS             complement(30153..32759)
                     /gene="yfhO"
                     /locus_tag="O2701_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosyltransferase
                     YfhO"
                     /protein_id="HCZ4292185.1"
                     /translation="MLKKWLNSNVKQFFVITFISVILTLILFSTHIYDYIVNGTVFSG
                     AGDGFRQMMPFQMYLYEHLRSFSSLYDASFGLGGDYMKGLSYYYSLSPLMWLNFLFIK
                     IGETVGIFNPTTIHFWPTNQLIMAMIRAIITFVVTFYLFKILHFKRSANMIATILYGM
                     STVVIYFNFTWSFYGNLLYLLPLSILGLERYFQQRKIGIFIVAIALTLFSNFYFSYYQ
                     AIIIGCYYLYRLIFTYKYDIVSRTQKLICVISATVLSVLSSVFGLFTGISAFLENDRK
                     QNPNVDIPFLTPLDYHYFFFSDGFYITISILTIVALLSFKLYRFYFYRLFAIVTWILF
                     IGSLSQYFDSAFNGFSFPERRWVYILALSSSALCGLFIQHLSTLNMKYYLIRTIPVCI
                     IAILYVLLSPTHPLALIVGIILLIVLAVILKFSLWRYKKLTVAILVLIVMIQQIVILD
                     NNKNMAIKPYQQSLSTLKQHDYHSNYVNQLIKKINQNATGSFNRIDYMSDYALNSPFI
                     YHYNGISLYSSIFNGDILKYYDKTLQINMPIDKNSTYRLLGNRQNLLSLWNVNDRIRV
                     NHDDNLPYGFKIKSEHKDNKVRWIHSKNTIHYPSAHITNKVFSNKELKSPLDKEQAML
                     QGIVSNNIKDVNTHFKANKNLLSDSTIKLNSAAWQSPTKHLLQVKQNNGGLTVQLPKS
                     VSNQFKDLYFEMDLELLSPDKAHDVKVNEYTQERNKLTYKYRRVVTPVTIRIKAPDRI
                     RLSLPKGKYRVNLKGIYGEDYTTLKDASNSLEAVKVSKTKHGYTITKNKNSSGYIVLP
                     TAYNQGMKATSGDQSLKVEQVNGVMTGIKAPKNITKIQLSYTPPYYYLLITITIFGII
                     CSIIFTRWARQK"
     gene            33160..34044
                     /gene="ylqF"
                     /locus_tag="O2701_000270"
     CDS             33160..34044
                     /gene="ylqF"
                     /locus_tag="O2701_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HCZ4292186.1"
                     /translation="MVIQWYPGHMAKAKREVSEQLKKVDVVFELVDARIPYSSRNPMI
                     DEVINQKPRVVILNKKDMSNLNEMSKWEQFFIDKGYYPVSVDAKHGKNLKKVEAAAIK
                     ATAEKFEREKAKGLKPRAIRAMIVGIPNVGKSTLINKLAKRSIAQTGNKPGVTKQQQW
                     IKVGNALQLLDTPGILWPKFEDEEVGKKLSLTGAIKDSIVHLDEVAIYGLNFLIQNDL
                     ARLKSHYNIEVPEDAEIIAWFDAIGKKRGLIRRGNEIDYEAVIELIIYDIRNAKIGNY
                     CFDIFKDMTEELANDANN"
     gene            34028..34795
                     /locus_tag="O2701_000271"
     CDS             34028..34795
                     /locus_tag="O2701_000271"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCZ4292187.1"
                     /translation="MTLTIKEVTQLINAVNTIEELENHECFLDERKGVQNAIARRRKA
                     LEKEQALKEKYVEMTYFENEILKEHPNAIICGIDEVGRGPLAGPVVACATILNSNHNY
                     LGLDDSKKVPVTKRLELNEALKNEVTAFAYGIATAEEIDEFNIYKATQIAMQRAIDGL
                     SVQPTHLLIDAMTLDNALPQVSLIKGDARSVSIAAASIMAKVFRDDYMTQLSKDYPEY
                     GFEKNAGYGTKQHLLAIDDIGIMKEHRKSFEPIKSLL"
     gene            34904..36070
                     /gene="sucC"
                     /locus_tag="O2701_000272"
     CDS             34904..36070
                     /gene="sucC"
                     /locus_tag="O2701_000272"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCZ4292188.1"
                     /translation="MNIHEYQGKEIFRSMGVAVPEGRVAFTAEEAVEKAKELNSDVYV
                     VKAQIHAGGRGKAGGVKIAKSLSEVETYAKELLGKTLVTHQTGPEGKEIKRLYIEEGC
                     AIQKEYYVGFVIDRATDQVTLMASEEGGTEIEEVAAKTPEKIFKETIDPVIGLSPFQA
                     RRIAFNINIPKESVNKAAKFLLALYNVFIEKDCSIVEINPLVTTADGDVLALDAKINF
                     DDNALFRHKDVVELRDLEEEDPKEIEASKHDLSYIALDGDIGCMVNGAGLAMATMDTI
                     NHFGGNPANFLDAGGSATREKVTEAFKIILGDENVKGIFVNIFGGIMKCDVIAEGIVE
                     AVKEVDLTLPLVVRLEGTNVELGKKILKDSGLAIEPAATMAEGAQKIVKLVKEA"
     gene            36092..37000
                     /gene="sucD"
                     /locus_tag="O2701_000273"
     CDS             36092..37000
                     /gene="sucD"
                     /locus_tag="O2701_000273"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCZ4292189.1"
                     /translation="MSVFIDKNTKVMVQGITGSTALFHTKQMLDYGTKIVAGVTPGKG
                     GQVVEGVPVFNTVEEAKNETGATVSVIYVPAPFAADSILEAADADLDMVICITEHIPV
                     LDMVKVKRYLQGRKTRLVGPNCPGVITADECKIGIMPGYIHKKGHVGVVSRSGTLTYE
                     AVHQLTEEGIGQTTAVGIGGDPVNGTNFIDVLKAFNEDDETKAVVMIGEIGGTAEEEA
                     AEWIKANMTKPVVGFIGGQTAPPGKRMGHAGAIISGGKGTAEEKIKTLNSCGVKTAAT
                     PSEIGSTLIEAAKEAGIYESLLTVNK"
     gene            37227..38345
                     /locus_tag="O2701_000274"
     CDS             37227..38345
                     /locus_tag="O2701_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCZ4292190.1"
                     /translation="MNKQQSKVRYSIRKVSIGILSISIGMFLALGMSNKAYADEIDKS
                     KDFTRGYEQNVFAKSELNANKNTTKDKIKNEGAVKTSDTSLKLDNKSAISNGNEINQD
                     IKISNTPKNSSQGNNLVINNNELTKEIKIANLEAQNSNQKKTNKVTNNYFGYYSFREA
                     PKTQIYTVKKGDTLSAIALKYKTTVSNIQNTNNIANPNLIFIGQKLKVPMTPLVEPKP
                     KTVSSNNKSNSNSSTLNYLKTLENRGWDFDGSYGWQCFDLVNVYWNHLYGHGLKGYGA
                     KDIPYANNFNSEAKIYHNTPTFKAEPGDLVVFSGRFGGGYGHTAIVLNGDYDGKLMKF
                     QSLDQNWNNGGWRKAEVAHKVVHNYENDMIFIRPFKKA"
     gene            38373..>39231
                     /gene="fmhC"
                     /locus_tag="O2701_000275"
     CDS             38373..>39231
                     /gene="fmhC"
                     /locus_tag="O2701_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhC"
                     /protein_id="HCZ4292191.1"
                     /translation="MKFSTLSEEEFTNYTKKHFKHYTQSIELYNYRNKINHEAHIVGV
                     KNDKNEVIAACLLTEARIFKFYKYFYSHRGPLLDYFDAKLVCYFFKELSKFIYKNRGV
                     FILVDPYLIENLRDANGRIIKNYNNSVIVKMLGKIGYLHQGYTTGYSNKSQIRWISVL
                     DLKDKDENQLLKEMEYQTRRNIKKTIEIGVKVEDLSIEETNRFYKLFQMAEEKHGFHF
                     MNEDYFKRMQEIYKDKAMLKIACINLNEYQDKLKIQLLKIENEMMTVNRALNENPNSK
                     KNKSKLNQLN"
BASE COUNT        14666 a         5248 c         7523 g        11794 t
ORIGIN      
        1 atttgattca aacaaaataa cttaagagga gaaagtttat gaaaaagaca ctgggatgtt
       61 tacttttaat tatgctttta gtcgtagcag gttgttcttt tggtgggaat cataaattat
      121 catcaaagaa atcagaagaa tcaaaacaag aaactgtaaa aaaagaatcg gaagaagaga
      181 aagatccaga tttagagaaa tatgaagaaa tagagaagaa aatgaaagga attaaagatg
      241 cgccatctct tgataagttg gatccattaa tgacagaaaa gtcgtttacg aatagtaaag
      301 ggattcaagg atggaaagat tacaaagaat taatgggtaa agtggaactt gcagattata
      361 gatttactaa agattcaaaa ggatcttcaa taaaagatgt tgatgcattc tttaaaggta
      421 agaaaggtat aaaaaggaaa gtgattgaaa cacacgatga tgtaaaacaa gttgattatt
      481 ggtatgtaga tccagatgga aagaaaattg gcaattcaaa cacacctgtt ttttacgcag
      541 aaattatgac aaaatataaa gatggaaagt tagtttatgc atcagtcgaa ccaggatctt
      601 acgtaataca taaagatgat gcaattaaat atgacgatta ttctaagtta aaaaaattaa
      661 gtcagctaac taaacttgat catccaaaac cagttccata tagcgtagct caaatcaaat
      721 ctttcggagt acctttaaca agcgtttcat ttatgacaca tggatcaaag gatactaaag
      781 atgaagtgtt gccggcattg gcctatttca ctttttcacc aaaaaattat gaagacaagt
      841 ctaatccaga tccaaaagtt ttaaatttag tacatatgga tttcttaaat gcatctagtg
      901 attttggtaa cgcacatttt gttgttttaa gtaaatatat taaagagtat gaatcaaact
      961 atgaaacagc gtcagatgat tctttaaaat agtatttact gtgtgaaaaa taaatagtgt
     1021 actacattaa ataatcgcaa taataatccc gataaacaat cagcattact gcttatcaca
     1081 tagagttcgt aataactata actctatgat tcgcaaataa taaatgattg tcatcgggat
     1141 ttatttttat caatttataa agtgacatta ccttgttcat cagcaggttt gaaaacagta
     1201 atcactgcac taataattgc taaaatgtgt gggatacctg tccaacagaa tattaagtgt
     1261 agaatacctt gcatattctt gccggcataa aatttatgaa taccaaaact acctaagaac
     1321 aatgctaata aaatataaat aactttgttt acttgcattt ctttccctcc agttgaattg
     1381 cttataatga cattagcttc tctttttatt atacccactt ttagttcaaa cattctagtt
     1441 taagcattcc caatcatcta aatttcagtt attcaatcct tacaataaat ttaggattac
     1501 atttcagttg cattgtatta ttttacgtgt gaaatatacg taatgaatca catgacaatc
     1561 ttcaaattga aaaatataca ttctatgatg taaggtcgca tttttaatat atttacgtta
     1621 taatagtttg atgaatttta ttgaggagta agagttatgg cgattaaaaa gttagtacca
     1681 gcatcgcatc ctattttaac gaaaaaagcg caagcagtta aaacatttga tgattcgtta
     1741 aaaagattat tacaagattt agaagataca atgtatgcac aagaagctgc tggcttatgt
     1801 gcacctcaaa ttaatcagtc attgcaagtg gcaattattg atatggaaat ggaaggatta
     1861 ttacaacttg ttaatccgaa aattattagt caatcaaatg aaacaataac agacttagaa
     1921 ggttcaatta cattgccaga tgtttacggc gaagtgacaa gaagtaaaat gatagttgtc
     1981 gaaagttatg acgtcaatgg gaacaaagtt gaactaactg cacatgaaga tgtagcaaga
     2041 atgattttgc atattataga tcaaatgaac ggtatccctt ttacagaacg tgcggaccgt
     2101 attttaacag ataaagaagt ggaggcatat tttataaatg actaaaataa tatttatggg
     2161 tacaccagac ttttcaacaa ctgttttaga aatgcttatt gcagaacatg atgtcattgc
     2221 agtcgtaacg caaccagatc gacctgttgg acgtaaacgt gttatgacac caccaccagt
     2281 taaaaaagtt gcaatgaaat atgatttacc tgtatatcaa cctgaaaaat taagtggatc
     2341 agaagaatta gaacaattgc ttcaattaga tgtagattta attgtaactg ctgcttttgg
     2401 acaattatta cctgaatcat tgttggcatt accaaatctt ggggcaatta atgtacatgc
     2461 atcattgtta ccgaagtata gaggtggtgc accaattcat caggcaatta tcgatggtga
     2521 acaagaaacc ggcataacaa ttatgtatat ggttaaaaaa ttagatgcgg gtaatattat
     2581 ttcgcaacaa gcaattaaaa tagaagaaaa tgataatgtc ggtacgatgc atgataaatt
     2641 aagtgtatta ggggcagatt tattaaaaga aactttacca tctattatag agggcacaaa
     2701 tgaaagcgta cctcaagatg atacgcaagc aacatttgct tccaatattc gacgcgaaga
     2761 tgagcgaatt agctggaata aaccaggaag acaagtgttt aatcaaattc gtggattatc
     2821 accatggcca gttgcttata caactatgga tgacactaac ttgaaaatat acgatgctga
     2881 actcgttgag actaataaga taaacgagcc tggaaccatt atagaaacga ctaaaaaagc
     2941 cattattgtt gctacaaatg ataatgaagc tgttgcaatt aaagatatgc aattagctgg
     3001 gaaaaagaga atgttagctg ccaattattt aagtggtgcg caaaacacac tagtagggaa
     3061 gaaacttata tgatagaaaa cgtgagaagt cttgcttttg acacgattca agatatatta
     3121 aatgaaggtg cgtatagtaa cttgcgtatc aatgaagtgt tgtcagaaaa tgaattaaat
     3181 gcaatggata aggctttatt tacagaaatt gtctacggaa ccgttaaaag aaaatatacg
     3241 ttagattttt atttaaagcc ttttgtgaaa acaaagatta aggcatgggt taggcaatta
     3301 ttatggatga gtatttatca atatgtttat ttagataaag ttccaaatca tgccattatt
     3361 aatgaagcag ttgaaatagc aaaagaacgc ggtggctatc ataatggtaa tgtcgtaaat
     3421 ggtattttac gtacaatgat gcgtagtgac ttacctgatt ttaatgaaat tgcagatcct
     3481 aaaaaaagaa tggcaatcga gtatagtatg ccgaagtgga ttatagatca ttgggcaaca
     3541 cattatggtc tcgaagaaac tgaaacaatt ttacagtcat ttttagaaac gacatcaaca
     3601 actgtgcgtg ccaacctgac gcgagcatca ttagatgata ttattgaaaa gttgcaagac
     3661 gaaggttatg acgttgaaaa agatcatgac ttaccttatt gtctccatat aggaggacaa
     3721 ccaattattc attctcgttc atttaaagat ggattcgttt caattcaaga taaaagctca
     3781 atgtttgttg cacacattat gaatgtagac cgacatgatc acgtattaga tgcatgtagt
     3841 gcacctggcg gtaaagcttg tcacattgct gaagttttaa tgccagaagg gcaagttgac
     3901 gcttcagata tacatgatca caaaatagac ttaattaatt ttaatataaa aaaattacga
     3961 ttaacaaata ttaaagcttt tcaacatgat gcgacaaaac cttatgataa aacatacgat
     4021 aagatacttg ttgatgcacc atgtagcgga ttaggtgtaa tgagacataa gccggagatt
     4081 aagtatactc aaagcaaaca acatattgag tcactagttg aattacagct tgaaatattg
     4141 gaaaatgtaa aaaacaatgt aaaaataggt ggcgaaatca tctattcaac atgtacaatt
     4201 gagcaactag aaaatgaaaa cgtgatttat acgtttttga aaaataataa aaacttcgaa
     4261 tttgaaccgt ttcaacatcc gataactgga gagttggtca aaacgttaca aatcatgccg
     4321 caagacttta attcagatgg attctttatc actaagataa aaagaaagga caattaggaa
     4381 tgataactgc tgaaaagaaa aagaagaata aatttcttcc aaattttgac aagcaatcaa
     4441 tatattcatt gcgatttgac gaaatgcaaa actggctcgt tgaacaaggg caacaaaaat
     4501 ttcgagcgaa acagattttt gaatggttat atcaaaaaag agtagattcg attgatgaaa
     4561 tgacgaactt atcgaaagac ttacgacagc ttttaaaaga taactttact gttacaactt
     4621 taacaactgt agtaaaacaa gaaagtaaag acggtacaat taaattctta tttgaattac
     4681 aagatggcta tacaattgaa actgttttaa tgagacatga ttatggaaat tcagtatgtg
     4741 taacgacaca agtaggttgt cgcatcggat gtacgttttg tgcttctaca cttggcggct
     4801 taaaaagaaa ccttgaagct ggcgaaattg tttcacaagt tttaacagtt caaaaagccc
     4861 ttgatgctac agaagagcgc gtatctcaaa ttgtcataat gggtatcggt gaaccatttg
     4921 aaaattatga tgaaatgatg gactttttaa gaatcgtcaa tgatgataac agtttaaata
     4981 ttggtgcacg tcacattaca gtatcaacat caggtatcat tcctagaata tatgactttg
     5041 cggatgaaga tattcaaatt aattttgctg taagcttaca cgccgcaaaa gatgaagtgc
     5101 gatcacgctt gatgccaatt aaccgtgcat ataatgttga gaagttaatc gaagcaattc
     5161 aatattatca agaaaaaaca aatcgtcgtg ttacttttga atatggtctg tttggtggtg
     5221 tgaatgacca actagaacat gcaagagaat tagcacattt aataaaaggc ttaaactgcc
     5281 atgttaactt aattcctgtc aaccatgttc cagaaagaaa ttatgtgaaa acggctaaaa
     5341 atgatatctt taaatttgaa aaagaattaa agagactagg aattaatgcc acaatacgtc
     5401 gtgaacaagg ttcggatatt gacgcagctt gtggtcaatt aagagcaaag gaacgacaag
     5461 tagaaacgag gtaaagacaa atgctagagg cacaattttt tactgatact ggacaacata
     5521 gagataagaa tgaagatgcg ggtggtattt tttataatca aactaatcaa caacttttag
     5581 ttctgtgtga tggtatgggt ggccataaag caggagaagt tgcaagtaaa tttgttacag
     5641 atgagttgaa atcccgtttt gaagcggaaa atcttataga acaacatcaa gctgaaaatt
     5701 ggttgcgtaa taatataaaa gatataaatt ttcagttata tcactatgca caagaaaatg
     5761 cagaatataa aggtatgggt acaacatgtg tttgtgcact tgtttttgaa aaatcagttg
     5821 tgatagcaaa tgtcggtgat tctagagcct atgttattaa tagtcgacaa attgaacaaa
     5881 ttactagtga tcactcattt gttaatcatc ttgttttaac gggtcaaatt acgccggaag
     5941 aagcatttac acatccacaa cgtaatatta ttacgaaggt gatgggcaca gataaacgtg
     6001 tgagtccaga tttgtttatt aagcgattaa atttttatga ttatttatta ttaaattcag
     6061 atggattaac tgattatgtt aaagacaatg aaattaagcg tttgttagta aaagaaggta
     6121 caatagaaga tcatggtgat caattaatgc aattggcatt agataaccat tcgaaagata
     6181 acgttacttt catactcgcg gctattgaag gtgataaagt atgataggta aaataataaa
     6241 tgaacgatat aaaattgtag ataagcttgg cggcggtggc atgagtaccg tttatcttgc
     6301 tgaagatacg atacttaaca ttaaagttgc aattaaggcg atttttatac cacctagaga
     6361 aaaagaagaa acattaaaac gttttgaacg agaagtacat aactcatcac agctatcaca
     6421 tcaaaatata gtaagtatga tcgatgttga tgaagaagat gactgttact acttagtaat
     6481 ggaatatatc gaaggtccga ctttgtctga gtatattgaa agtcatgggc cattaagtgt
     6541 tgacacagcg attaatttta cgaatcaaat attggatggc attaaacatg cgcatgatat
     6601 gcgtattgta catagagata ttaagccaca aaatatatta attgacagca ataaaacgtt
     6661 gaaaatattt gattttggaa ttgctaaagc tttaagtgag acgtctttaa ctcagactaa
     6721 tcatgtgtta ggtactgtgc agtacttttc gccagaacaa gcaaaaggtg aggcaacgga
     6781 tgaatgtaca gatatttatt ctataggtat tgtgttatat gaaatgcttg ttggtgaacc
     6841 accctttaat ggagaaactg cagttagcat tgcgattaaa catattcagg attctgtgcc
     6901 aaatgtgaca acagatgtac gtaaggatat tccgcaatct ttaagtaatg tcattttacg
     6961 cgctacagaa aaagacaaag cgaatcgtta caaaacaatt caagaaatga aagatgattt
     7021 gagtagtgtt ttacatgaaa atcgagcgaa tgaagatgtc tatgaactcg ataaaatgaa
     7081 aacgatagcg gtacctttga aaaaagaaga tctagcaaag catattagtg aacataagtc
     7141 gaatcaacct aaacgtgaaa cgacgcaagt acctattgta aatgggcctg ctcatcatca
     7201 gcaattccaa aagccagaag gtacggtgta cgaaccaaaa cctaaaaaga aatcaacacg
     7261 aaagattgtg ctcttatcac taatcttttc gttgttaatg attgcacttg tttcttttgt
     7321 ggcaatggca atgtttggta ataaatacga agagacacct gatgtaatcg ggaaatctgt
     7381 aaaagaagca gagcaaatat tcaataaaaa caacctgaaa ttgggtaaaa tttctagaag
     7441 ttatagtgat aaatatcctg aaaatgaaat tattaagaca actcctaata ctggtgaacg
     7501 tgttgaacgt ggtgacagtg ttgatgttgt tatatcaaag ggccctgaaa aggttaaaat
     7561 gccaaatgtc attggtttac ctaaggagga agccttgcag aaattaaaat cgttaggtct
     7621 taaagatgtt acgattgaaa aagtatataa taatcaagcg ccaaaaggat acattgcaaa
     7681 tcaaagtgta accgcaaata ctgaaatcgc tattcatgat tctaatatta aactatatga
     7741 atctttaggc attaagcaag tttatgtaga agactttgaa cataaatcct ttagcaaagc
     7801 taaaaaagcc ttagaagaaa aagggtttaa agttgaaagt aaggaagagt atagtgacga
     7861 tattgatgag ggtgatgtga tttctcaatc tcctaaagga aaatcagtag atgaggggtc
     7921 aacgatttca tttgttgttt ctaaaggtaa aaaaagtgac tcatcagatg tcaaaacgac
     7981 aactgaatcg gtagatgtac catacactgg taaaaatgat aagtcacaaa aagttaaagt
     8041 ttatattaaa gataaagata atgacggttc aactgaaaaa ggtagtttcg atattactag
     8101 tgatcaacgt atagacattc ctttaagaat tgaaaaagga aaaacagcaa gttatattgt
     8161 taaagttgac ggtaaaactg tagctgaaaa agaagtcagc tatgatgatg tataaatata
     8221 attgaagtaa atgtaccgag gtttctattt ggaagtctcg gtatttttat gttgagattg
     8281 cggtagtttt aaaatgcttc atgtcatcat atacaggtga gcattgtcta tcaattctgt
     8341 atcactctca caatatttat atcatttgaa atgatcattt atatattaat tttccagtct
     8401 ttgatataat aaatgcagta aaattaatcg agaggtgcca ttttgaagac aggtcgaata
     8461 gtgaaatcaa ttagtggggt atatcaagta gacgttaatg gcgaacgttt caatacaaaa
     8521 ccacgaggat tatttagaaa gaaaaaattt tcaccggtag ttggtgatat agtggaattt
     8581 gaagttcaaa acattaacga aggttatatt catcaagtgt ttgagcggga aaatgagttg
     8641 aaaagaccac ctgtaagtaa tatagataca ctagtaattg taatgagtgc tgtcgagcca
     8701 aatttttcaa cgcaattatt agatcgattt ttagttattg cacattcgta tcagttaaat
     8761 gcgagaattt tggtgactaa aaaagataaa acaccaattg aaaagcagtt cgaaataaat
     8821 gagttgttga aaatatatga aaatattggc tatgagactg aatttattgg aaatgatgat
     8881 gatcgaaaaa aaattgtaga agcttggcca gctggactta tagtacttag tggtcaatca
     8941 ggtgtcggta agtccacttt cttaaatcat tatcgtccag aacttaatct tgagacaaat
     9001 gatatatcaa aatcattaaa tcgaggaaag catactacaa gacatgtcga actattcgaa
     9061 cgtcaaaacg gttatattgc agacacacct ggattcagtg ctttagattt tgatcatata
     9121 gataaagatg aaataaaaga ttattttctt gaattaaatc gatatggtga aacatgtaag
     9181 tttaggaatt gtaatcatat caaagaacct aattgtaatg ttaagcatca attagagata
     9241 gggaatattg cgcaatttag atacgaccat tatttacaac tatttaatga aatttcaaat
     9301 agaaaggtta gatattaaat gacaaaacta tatccatcat tattatctgt tgattttttg
     9361 gatttacaac atgaattaaa acgacttgaa gaagcaggcg tcgacggagt tcattttgat
     9421 gttatggatg gtcaatttgt gcctaatata tctattggtt taccaatatt agatgcagta
     9481 agaaaaggca caacattacc tatagacgta catttgatga ttgaaaatcc agaaaagtat
     9541 attgcatcat ttgcagaaca tggtgccgat atgatttcaa ttcatgtcga atcaacgcct
     9601 catattcatc gtgctattca aatgattaaa catttagata aaaaagctgg tgtagtaatt
     9661 aatcctggta caccaatatc acaaattgaa cctattttag acattgttga ttatgtacta
     9721 gtgatgacag ttaacccagg gtttggtggt caatcattta ttgatcaatg cgtagaaaaa
     9781 atagcgggtc ttaatgctat taaaatggaa cgtcaattaa actttgatat tgaagttgat
     9841 ggaggcgtaa ataccgatac agcgaaagtt tgtgttgaaa atggtgctac aatgctagta
     9901 acaggttcat ttttctttaa acaagaggat tataaaaaag tcacacaagc attgaaaggt
     9961 tgagtgtata tgcatataaa tttattatgt tctgatcgac acttgccgca agatatttgg
    10021 gccaaaagta atgaaggtaa atggggcggc gttgatagag gtgctttgat tttattgaag
    10081 catcaaatta tccctttttt ctcagtggga gactttgatt cagtcagtaa agaagaacgc
    10141 caacttctaa cagaacagtt acaaatcaaa ccagttcaag ctgaaaaagc tgatacggat
    10201 ttagctttag cggttgataa agctgttgca cttggatttg atagtattac aatttatggt
    10261 gcaacaggcg gacgattaga tcactttttt ggggcaattc agttattatt gaaaaaagca
    10321 tattataaac atgatgttca tatagaagtt atcgatcaac aaaataaaat tgaattattg
    10381 cctaaaggtc aacatacagt tgaaaaagat aagagttatc cgtacatttc atttataccg
    10441 atgactgatg atgtagaact ttctctagca ggttttaaat ataatttagc tagacaaatg
    10501 cttaatatag gttctacttt aactatttca aatgaaattg agtctttgca agcgaaagta
    10561 actgtacatg atgggttgat tttgcaaatt agaagtacag atttaaatta agtatattca
    10621 aatttgtgag gatttttaat aaagtgcatg agttatttaa taaagtgacg tttatagata
    10681 gaatttataa cataataata gatgaatcac gctgtaatta atgaagatta tggcgtgatt
    10741 tattattgaa aggaaaattg taggtcctag tacattgatt tatgtgccaa aatcgatatt
    10801 gaacccataa gtccaatttg acagagaacc attattaaac gtattaacca aattatattg
    10861 atagttcgaa tttcactatg aaccattatg tattgtttgg taacaaaaaa agaactaaga
    10921 ggttgagctc ttagttctta agtaaatgtg tcctccacat aaggtcacat tttttatgtg
    10981 caatatattt attaaactct agttacttta ccagatttta aagcacgtgc agaaacccaa
    11041 acttttttag gtttaccgtc aactaggatt ctaacttttt gaaggttagc gttccatcta
    11101 cgtttagtag agtttaaagc gtgtgaacgt ctgttaccag tcgaagcttt acgacctgtt
    11161 acgaaacatt gtttacccat atgagtacct cctttaataa atataaatac acataactac
    11221 ttatatactt aattaagata gcatagtttc atttgaaaaa caatgaataa ttttcacata
    11281 aaagtcaaaa atactgagtt ttgtgatata attgtagact gtgaagttat gtagtatgat
    11341 attttagaga aataagtgaa tgaaacattt aaaattttta tttaatgata ctacatctat
    11401 taaaagaaaa cgcctataga taatcgtcat gagtgtaaat tcagtttact caaaatataa
    11461 taggtgattt aagcgtaagt cattctgata agttgtacca ttgatatttt ataaaaataa
    11521 tcaatgaaac ttaaagcgtt tatgactaca ctaagtggta aaattttaaa agtgattagg
    11581 gaaaacgaat aataaacttt gaaatttagg agggacaaag atatgacatt agagatttca
    11641 aatgattacg gtaaaattga tatttcaaac gaagtgattg cttcggttgt aggtggaaag
    11701 gccgttgaat gttatggtat tgtaggtatg gcatctagac aacaagttag agatggtatt
    11761 gcggaaatac taggacatga aaattatgct aaaggcatca aagtaactga aaataatggc
    11821 gtagtggata tagatatgta cattattgta agttacggtg tgaaaatatc tgaagttgcc
    11881 aataatgtac aatcaacagt gaaatatact ttggaaaaat cacttaatgt atcagtaaat
    11941 tcaatcaata tatatgtaca aggtgtacgt gtgaataata caggcaagaa agcttaggag
    12001 gacaacttga aatgattagc aaaattaatg gtaaattatt tgccgatatg attatacaag
    12061 gggcacaaaa tttatctaac aatgcagatt tggtagattc tttgaatgtg tatccagtgc
    12121 cagatggtga tacaggaaca aatatgaatc ttactatgac ttcaggtcgc gaagaagtag
    12181 agaataattt gtcgaaaaat atcggcgaat taggtaaaac attctcgaaa ggtttactaa
    12241 tgggtgcaag aggtaactct ggtgtcatct tgtcacaatt attcagagga ttttgtaaaa
    12301 atattgaaag tgaatctgaa attaattcaa aattgttagc tgaaagtttt caagctggtg
    12361 ttgaaacggc atataaagct gttatgaaac cagttgaagg tacaatactt acagttgcaa
    12421 aagatgctgc gcaagctgca atagaaaaag caaataatac tgaagattgt atagaattaa
    12481 tggagtacat tattgtaaaa gccaatgaat cacttgaaaa cacaccaaac ttattagctg
    12541 tacttaaaga agttggtgtt gttgatagtg gcggtaaagg tttgttatgc gtttacgaag
    12601 gattcttaaa agcgcttaaa ggtgaaaaag ttgaagccaa agttgcaaag atagataaag
    12661 atgaatttgt acatgatgaa catgatttcc atggtgtaat taatactgaa gatattattt
    12721 atggctattg tactgaaatg atggttcgtt ttggaaagaa taaaaaagcc tttgatgaac
    12781 aagaattcag gcaagatatg agtcaatttg gtgattcttt attagtcatt aatgatgaag
    12841 aaattgtgaa agttcacgtg cataccgaat acccaggtaa agtgtttaat tatggtcaac
    12901 aatatggtga attaattaaa cttaaggttg aaaatatgag agaacagcac cgtgaagtga
    12961 ttcgaaaaga acagcacaca gctaaaccga aaatggaaac ggttgaaaca gcaattatta
    13021 ctatttctat gggtgaaggt atttcagaga tatttaaatc aatgggtgcc acacatatca
    13081 ttagtggtgg acaaacgatg aatccttcta cagaagatat cgttaaagtc attgaacaat
    13141 caaaatgtaa acgtgcaatt attttaccga ataataaaaa tatcttaatg gcaagtgaac
    13201 aagcagcgag tattgttgat gcagaagctg ttgttattcc aacgaaatct attcctcaag
    13261 gtataagcgc actattccaa tatgatgtgg acgcaacact tgaagaaaat aaagcgcaaa
    13321 tggctgattc agtaaataac gttaaatctg gttcattaac gtacgctgtt cgtgatacga
    13381 aaattgatgg cgttgagatt aaaaaagacg cgtttatggg cttgattgaa gataagattg
    13441 taagcagcca aagtgatcaa ttaacaacgg ttactgagtt gttaaatgag atgttagcag
    13501 aagatagtga aatattgact gtgattattg gtcaagatgc agagcaagca gttacagata
    13561 acatgataaa ctggatcgaa gagcaatatc cagatgtaga agtggaagtt catgaaggtg
    13621 gacaaccaat ttatcaatat ttcttttcag tagaataaaa atttaaaata aaaaactacc
    13681 aatgataaat catcagttgg tagtttttta ttttgctatt ttagtgatat tgcgggttaa
    13741 aagtatcgtt ctcgagttgc taacaatgtc atgttcaact tagtcatgat aaaataaata
    13801 acatactaaa tgatacgtaa aatcaaataa aacataggtg atttattttg gctaaagtaa
    13861 acttaataga aagtccatat tctcttttac aattaaaagg tataggtcct aagaaaatag
    13921 aagtattgca acaactaaat attcatacag tggaagatct tgttctttat ttgccaacta
    13981 gatatgaaga taatacagtg attgatttga atcaagcaga agatcaatct aacgttacga
    14041 tagaaggaca agtatataca gctccagtag ttgcattttt tggaagaaat aaatcaaaat
    14101 taaccgttca tttaatggta aataatattg ctgtcaaatg tatttttttc aatcaaccgt
    14161 atttaaaaaa gaaaatcgaa ttaaatcaaa ctataactgt taaaggtaag tggaataggg
    14221 ttaaacagga aattactggt aatagggttt tctttaattc acaagggaca caaactcaag
    14281 aaaacgcaga tgttcaatta gaaccagtct atcgtattaa ggaaggtatt aaacaaaagc
    14341 aaatacgaga ccaaattaga caagcgttaa atgatgtgac aattcatgaa tggttaactg
    14401 atgaactaag agaaaaatat aaattagaga ccttggactt tactttgaac acattacatc
    14461 atcctaaaag taaagaggat ttattacgtg ctcgtagaac ctatgcattt actgaactgt
    14521 ttttattcga attacgtatg caatggctaa atagattaga aaagtcatct gacgaagcaa
    14581 ttgaaattga ttatgacata gaccaagtta aatcatttat tgatcgttta ccttttgaac
    14641 taactgaagc acagaaatcc agtgttaatg aaatttttag agatttaaaa gcaccaatac
    14701 gtatgcatcg attacttcaa ggtgatgtag gttcaggaaa aacagtagtt gctgcaattt
    14761 gtatgtatgc gttaaaaact gctggttatc aatcagcatt gatggtacca actgaaattt
    14821 tagcagagca acatgctgaa agtttaatgg ctttatttgg agattctatg aacgttgcat
    14881 tgttaactgg gtcagtaaaa ggtaagaaac gaaagatact tttagaacaa cttgaaaatg
    14941 gtacgattga ttgtttaatt ggaacccatg ctttgattca agatgatgtg attttccata
    15001 atgttggttt agtaattaca gatgaacaac atcgatttgg tgtgaatcaa cgccagcttt
    15061 taagagaaaa aggtgcaatg acgaatgtgt tatttatgac agcaacgccg ataccaagaa
    15121 cactagcaat atcagttttt ggtgagatgg atgtgtcttc aattaaacaa ttaccaaaag
    15181 gtcgtaaacc tatcattact acttgggcaa agcatgagca atacgataaa gttttgatgc
    15241 aaatgacctc agagttgaaa aaaggtcgtc aagcatatgt catttgcccg ctaatagaaa
    15301 gttctgagca tctcgaagat gttcaaaatg ttgtcgcatt gtacgagtct ttacaacagt
    15361 attatggtgt ttcccgtgta gggttattgc atggtaagtt atctgccgat gaaaaagatg
    15421 aggtcatgca aaagtttagt aatcatgaga taaatgtttt agtttctact actgttgttg
    15481 aagtaggtgt taatgtaccg aatgcaactt ttatgatgat ttatgatgcg gatcgctttg
    15541 gattatcaac tttacatcag ttacgcggtc gtgtaggtag aagtgaccag caaagttact
    15601 gtgttttaat tgcatcccct aaaacagaaa caggaattga aagaatgaca attatgacac
    15661 aaacaacgga tggatttgaa ttgagtgaac gagacttaga aatgcgtggt cctggagatt
    15721 tctttggtgt taaacaaagt ggattgccag atttcttagt tgccaattta gttgaagatt
    15781 atcgtatgtt agaagttgct cgtgatgaag cagctgaact tattcaatct ggcgtattct
    15841 ttgaaaatac gtatcaacat ttacgtcatt ttgttgaaga aaatttatta catcgtagtt
    15901 ttgactaatt gccatgctga tttgtcaatt tgagtgcaac acttcgttaa ttgagtgata
    15961 tgacacttga actatttaaa tgtaaagtgg tattttaaca atttataaat tttcgactaa
    16021 ataatagcta aatattacag ttatttgttg agtcggttaa atagaaagtg ttatgatatg
    16081 tgaggaatgt ttaagactag gtactaaaaa atgaggggtg agacgttgaa actaaagaaa
    16141 gataaacgta gagaagcaat cagacaacaa attgatagca atcccttcat cacagaccat
    16201 gaactaagcg acttatttca agtgagtata caaacaattc gtttagatcg cacttattta
    16261 aacataccag aattaaggaa gcgtattaaa ttagttgctg aaaagaatta tgaccaaata
    16321 agttctattg aagaacaaga atttattggt gatttgattc aagtcaatcc aaatgttaaa
    16381 gcgcaatcaa ttttagatat tacatcggat tctgtttttc ataaaactgg aattgcgcgt
    16441 ggtcatgtgc tgtttgctca ggcaaattcg ttatgtgttg cgctaattaa gcaaccaaca
    16501 gttttaactc atgagagtag cattcaattt attgaaaaag taaaattaaa tgatacggta
    16561 agagcagaag cacgagttgt aaatcaaact gcaaaacatt attacgtcga agtaaagtca
    16621 tatgttaaac atacattagt tttcaaagga aattttaaaa tgttttatga taagcgagga
    16681 taaaattatg gttaaattag caattgatat gatgggtggc gacaatgcgc ctgatatcgt
    16741 attagaagcc gtacaaaagg ctgttgaaga ctttaaagat ctagaaatta tacttttcgg
    16801 tgacgaaaaa aagtataatc tgaaccatga acgaatcgaa tttagacatt gttctgaaaa
    16861 gattgaaatg gaagatgagc ctgttagagc gattaaacgt aaaaaagata gctcaatggt
    16921 aaaaatggct gaagctgtga aatctggtga agcagatgga tgtgtgtcag caggtaatac
    16981 tggtgcttta atgtcagctg gtttattcat tgttggacgt attaaaggtg tagctagacc
    17041 ggctttagta gtaacattgc caacgattga tggaaaaggt tttgtctttt tagacgttgg
    17101 tgcaaatgct gatgctaaac ctgaacactt attacagtat gcgcaactag gggatattta
    17161 tgctcaaaaa attagaggta ttgataatcc gaaaatctca ttattaaata taggaaccga
    17221 gccagctaaa ggtaatagtt taacgaaaaa atcatatgag ttattaaatc atgatcattc
    17281 attgaatttt gttgggaata ttgaagcgaa gacattaatg gatggcgata cagatgttgt
    17341 agttaccgat ggctatactg ggaacatggt ccttaaaaat ttagaaggta ctgcaaaatc
    17401 aatcggtaaa atgttaaaag atacgattat gagtagtact aaaaataaat tagcaggtgc
    17461 aatattgaag aaagatttag ctgaattcgc taaaaagatg gattactcag aatacggtgg
    17521 ttccgtatta ttaggattgg aaggtactgt agttaaagca cacggtagtt caaatgctaa
    17581 agctttttat tctgcaatta gacaagcgaa aatcgcagga gaacaaaata ttgtacaaac
    17641 aatgaaagag actgtaggtg aatcaaatga gtaaaacagc aattattttt ccgggacaag
    17701 gtgcccaaaa agttggtatg gcgcaagatt tgtttaacaa caatgatcaa gcaactgaaa
    17761 ttttaacttc agcagcgaac acattagact ttgatatttt agagacaatg tttactgatg
    17821 aagaaggtaa attgggtgaa actgaaaaca cacaaccagc tttattgacg catagttcgg
    17881 cattattagc agcgctaaaa aatttgaatc ctgattttac tatggggcat agtttaggtg
    17941 aatattcaag tttagttgca gctgacgtat tatcatttga agatgcagtt aaaattgtta
    18001 gaaaacgtgg tcaattaatg gcgcaagcat ttcctactgg tgtaggaagc atggctgcag
    18061 tattgggatt agattttgat aaagtcgatg aaatttgtaa gtcattatca tctgatgaca
    18121 aaataattga accagcaaac attaattgcc caggtcaaat tgttgtttca ggtcacaaag
    18181 ctttaattga tgagctagta gaaaaaggta aatcattagg tgcaaaacgt gtcatgcctt
    18241 tagcagtatc tggaccattc cattcatcgc taatgaaagt gattgaagaa gatttttcaa
    18301 gttacattaa tcaatttgaa tggcgtgatg ctaagtttcc tgtagttcaa aatgtaaatg
    18361 cgcaaggtga aactgacaaa gaagtaatta aatctaatat ggtcaagcaa ttatattcac
    18421 cagtacaatt cattaactca acagaatggc taatagacca aggtgttgat cattttattg
    18481 aaattggtcc tggaaaagtt ttatctggct taattaaaaa aataaataga gatgttaagt
    18541 taacatcaat tcaaacttta gaagatgtga aaggatggaa tgaaaatgac taagagtgct
    18601 ttagtaacag gtgcatcaag aggaattgga cgtagtattg cgttacaatt agcagaagaa
    18661 ggatataatg tagcagtaaa ctatgcaggc agcaaagaga aagctgaagc agtagtcgaa
    18721 gaaatcaaag ctaaaggtgt tgacagtttt gcgattcaag caaatgttgc cgatgctgat
    18781 gaagttaaag caatgattaa agaagtagtt agccaatttg gttctttaga tgttttagta
    18841 aataatgcag gtattactcg cgataattta ttaatgcgta tgaaagaaca agagtgggat
    18901 gatgttattg acacaaactt aaaaggtgta tttaactgta tccaaaaagc aacaccacaa
    18961 atgttaagac aacgtagtgg tgctatcatc aatttatcaa gtgttgttgg agcagtaggt
    19021 aatccgggac aagcaaacta tgttgcaaca aaagcaggtg ttattggttt aactaaatct
    19081 gcggcgcgtg aattagcatc tcgtggtatc actgtaaatg cagttgcacc tggttttatt
    19141 gtttctgata tgacagatgc tttaagtgat gagcttaaag aacaaatgtt gactcaaatt
    19201 ccgttagcac gttttggtca agacacagat attgctaata cagtagcgtt cttagcatca
    19261 gacaaagcaa aatatattac aggtcaaaca atccatgtaa atggtggaat gtacatgtaa
    19321 tatatttgag ctaaagctca ttgacgcagt ggttgactgg tcatccaatg gagaattgtc
    19381 tgacctagtc aactttgcgg gggaaattct aagcaaccta gataaggttc cagaatttct
    19441 ccctaagaaa cactaatcaa taaattgata agtgtttcta aaatttctac ttgtttttta
    19501 gaatttaaaa tgggaaaata tagtagtcta tgtataggca tttttaaagg aggtgaatcg
    19561 acgtggaaaa tttcgataaa gtaaaagata tcatcgttga ccgtttaggt gtagacgctg
    19621 ataaagtaac tgaagatgca tctttcaaag atgatttagg cgctgactca cttgatatcg
    19681 ctgaattagt aatggaatta gaagacgagt ttggtactga aattcctgat gaagaagctg
    19741 aaaaaatcaa cactgttggt gatgctgtta aatttattaa cagtcttgaa aaataataaa
    19801 tcttacatct gggtcgtcag tattgtcgac tcagtttttt tctttaatta tcaatagttt
    19861 taacgtaaaa ttaaagatga ttcaagagca acacataaag gagataaaat aatgtctaaa
    19921 caaaagaaaa gtgagatagt taatcgtttt agaaagcgct ttgatactaa aatgacagag
    19981 ttaggcttta cttatcaaaa tattgattta taccaacaag cattttcgca ttcgagtttt
    20041 attaatgatt ttaatatgaa tcgtttagac cataatgagc gtttagagtt tttgggtgat
    20101 gcggtattag aattgacggt ttcacgatat ttatttgata aacatcccaa cttgccagaa
    20161 gggaatttaa caaaaatgcg tgccactatt gtatgtgagc cctcacttgt aatatttgcg
    20221 aataaaattg gattgaacga aatgatttta cttggtaaag gtgaagagaa aacaggggga
    20281 cgtacaagac catcattaat atcagatgca ttcgaagcat ttattggggc attgtatttg
    20341 gatcaaggac tagatatagt ttggaaattt gctgagaaag tcattttccc acatgtagaa
    20401 caaaatgagt tattaggcgt ggtagatttt aaaacacaat tccaagaata tgtgcaccag
    20461 caaaataaag gtgatgtaac ctataattta ataaaagaag agggaccggc acatcatcgt
    20521 ctattcactt cagaagttat tctgcaaggg gaagcaatag ctgaaggtaa agggaaaacg
    20581 aaaaaagaat cagaacaacg tgctgctgaa agtgcctata agcaattaaa acaaattaaa
    20641 tagaaattta tatgatacga catataggtg ttcgatttag gatttaagga agaattttta
    20701 atattttatg gaacaacttc gaaatttaat atacaaatac taaaatatcg tatacatgtg
    20761 ttctttaaaa ttgtgataag gagtttagga tggtttattt aaaatcaata gatgccattg
    20821 gatttaagtc ttttgcagat caaaccaatg ttcaattcga taaaggtgta actgcaattg
    20881 ttggtccaaa tggaagcggt aaaagtaata ttacagatgc tattaaatgg gtgttgggtg
    20941 aacaatcggc taaatcatta cgtggctcaa aaatggaaga tattatcttc tcaggtgcag
    21001 aacatcgcaa agctcaaaat tatgctgaag tacagttaag attagataat cattctaaaa
    21061 agctcagtgt tgatgaaaac gaagttattg taacaagaag attgtatcga agtggtgaaa
    21121 gtgagtacta cataaataat gaccgtgcaa gattaaaaga tattgccgat ttatttttag
    21181 attctggatt gggaaaagaa gcgtatagca ttatctcgca aggtagagtt gatgaaatac
    21241 taaatgctaa accaattgat agacgtcaaa ttattgaaga atcggctggt gtacttaaat
    21301 ataaaaaacg taaggctgaa tcattaaata aacttgacca aacagaagat aatttaacga
    21361 gagtagaaga cattttatat gatttggaag gtcgcgtaga acctctaaaa gaggaggcag
    21421 ctatagctaa agaatataag acactttcac atcaaatgaa acatagtgac attgtagtta
    21481 cagtgcacga tattgatcaa tatacaaatg acaatagaca attagatcaa cgtttaaatg
    21541 atttacaagg ccaacaagca aataaagaag ctgacaagca acgtttaagc caacaaattc
    21601 aacaatataa aggtaaacgt catcaacttg ataatgatgt tgaatcgctt aattatcaat
    21661 tagtaaaagc tacggaagcc tttgaaaaat atacgggaca attaaatgtt ttggaagaac
    21721 gtaagaaaaa tcaatctgaa acaaatgcac gatatgaaga agaacaagaa aatttaatgg
    21781 agcttttaga aaatatatca aatgagattt ctgaagctca agatacttat aagtctctga
    21841 aaagtaaaca aaaagaactc aatgctgtca ttcgtgaact tgaagaacaa ctatatgttt
    21901 cagacgaagc acatgatgaa aaattggaag aaattaaaaa cgaatactat acattaatgt
    21961 cagagcaatc agatgttaac aatgatattc gttttttaaa gcatactata gaagagaatg
    22021 aggctaaaaa atcaagacta gattctcgat tagttgaagt ttttgagcaa ttgaaagata
    22081 ttcagggtca aataaaaacg acaaaaaaag aatatcaaca gaccaacaaa gaactttctg
    22141 ctgtagataa agaaattaaa aatatagaaa aagacctcac tgatacaaaa aaagcacaaa
    22201 atgaatacga agagaaattg tatcaagcat atcgatatac cgaaaaaatg aaaacacgta
    22261 ttgatagttt ggcaacgcaa gaggaagaat atacttattt tttcaatggc gtcaaacata
    22321 ttttgaaagc taaaaataaa gaattaaagg gtatttatgg tgcagttgcg gaaattattg
    22381 atgtgccatc taaattaact caggcaattg aaacagcatt aggtgcttca ttacaacatg
    22441 tcattgtaga ttcagaaaaa gatggacgcc aggctattca atttttaaaa gaacgtaatt
    22501 taggtcgtgc gacgttttta ccattaaatg ttatacagag tagagtggta gcgactgata
    22561 ttaaatctat tgctaaagag gcaaacggat ttattagtat cgcttcggaa gcagttaaag
    22621 tagcaccaga atatcaaaat attatcggga atttattagg taatacgatt atcgttgatc
    22681 atttaaagca tgcaaatgaa ttggcacgtg cgattaaata tcgaactcgt attgttactt
    22741 tggaaggtga tattgtaaat cctggtggtt ctatgactgg tggtggcgct cgtaagtcaa
    22801 aaagtattct gtctcaaaaa gacgagttga caacaatgag acaccaatta gaagattact
    22861 tgcgtcaaac agaatcattt gaacaacaat ttaaagagtt gaagataaaa agtgatcaat
    22921 taagtgaact gtattttgaa aaaagtcaaa agcataatac acttaaagag caagtgcatc
    22981 attttgaaat ggagctcgat agattaacta cacaagaaac acaaataaaa aatgatcatg
    23041 aagaattcga atttgaaaaa aatgatggtt atacgagtga caaaagtcga caaactttga
    23101 gtgaaaaaga aacttatcta gaaagtatta aagcatcttt aaaacgacta gaagatgaaa
    23161 ttgaacgcta cacaaaactt tctaaagaag gtaaggaaag cgttactaaa acacaacaaa
    23221 ccttacatca gaaacaatct gatcttgctg tggttaaaga gcgtattaaa acacaacaac
    23281 agacaataga tcgattaaat aatcaaaatc aacaaactaa acatcaatta aaagatgtta
    23341 aagaaaaaat tgcattcttt aattcggatg aagtgatggg cgaacaagct tttcaaaata
    23401 ttaaagatca aattaatggt caacaagaaa cgagaacacg cttatcagat gaattagata
    23461 aattgaaaca acaacgtatt gagttgaatg aacaaatcga tgcgcaagaa gctaaactac
    23521 aagtttgtca ccaagatatt ttagctatcg aaaatcacta ccaagatatt aaagctgaac
    23581 aatcaaagct agatgtatta attcatcatg cgatagatca tttaaatgat gaatatcaat
    23641 tgactgttga acgtgcgaaa tctgaatata cgagtgatga atcgattgac gcattacgta
    23701 aaaaagttaa gttaatgaag atgtcgattg atgaactagg tcctgtaaac ttaaatgcaa
    23761 ttgaacaatt tgaagagtta aatgaacgtt atacattttt aagtgaacaa cgtacagatc
    23821 ttcgtaaagc taaagaaaca ttagagcaaa ttataagtga aatggatcaa gaggttactg
    23881 aaagatttaa agaaactttc catgctattc aaggacattt tacagctgtg ttcaaacaat
    23941 tgtttggtgg aggcgatgca gaattgcaat taactgaagc cgattattta acagctggta
    24001 ttgatattgt ggtacaacca ccgggtaaaa agttgcaaca tttatcgtta ctgagtggtg
    24061 gtgagcgtgc attaactgct attgctttac tatttgcaat tttaaaagta agatctgcac
    24121 cttttgttat attagatgag gttgaagctg cactagatga agcaaatgtt attagatacg
    24181 caaaatattt aaatgagtta tcagacgaaa cacaattcat tgttattaca caccgtaaag
    24241 gaacaatgga atttgcagat aggttatacg gtgtaacaat gcaagaatca ggtgttacta
    24301 aacttgtgag tgtgaattta aatacaatag atgatgtgtt gaaggaggag caataatgag
    24361 cttttttaaa cgcttaaaag ataagtttgc aacaaataaa gaaaatgaag aagttaaatc
    24421 cttaacagaa gaacaaggtc aagacaaatt agaagataca cattctgaag gttcaacgca
    24481 ggacgcaaat gatttagcag aaaatgctga agtgaaaaag aagccacgca agttgagtga
    24541 agcggatttt gatgacgatg gcttaatatc aattgaagat tttgaagaaa ttgaagctca
    24601 aaaaatgggt gctaaattta aagcaggact cgaaaaatct cgtcaaaatt tccaagaaca
    24661 attaaataat ttgatagcga gatatcgtaa agtagatgaa gacttttttg aagctttaga
    24721 agaaatgtta atcactgcag acgtcggttt taatacagtg atgacgttaa ctgaagaatt
    24781 acgtatggaa gcacaacgac gtaatattca agatactgaa gatttgcgtg aagtcattgt
    24841 tgaaaaaatc gtagagattt accatcaaga agatgataat tcagaagcta tgaacttaga
    24901 agatggtcgt ttaaatgtca ttttaatggt tggtgtgaat ggtgttggta aaacaacaac
    24961 aattggaaaa ttagcttacc gatataaaat ggaaggtaaa aaagtaatgt tagctgcggg
    25021 cgatactttt agagcgggtg ctattgatca attgaaagtt tggggcgaac gtgttggtgt
    25081 agacgtaatt agccaaagtg aaggttctga tccagctgct gttatgtatg atgcgattaa
    25141 tgccgctaaa aacaaaggtg ttgatatttt aatctgtgat accgctggac gtttacaaaa
    25201 taaaacaaat ctaatgcaag aattagaaaa agttaagcgt gtaattaatc gagcagtgcc
    25261 agatgcgcct catgaagcat tactatgttt agatgctaca actggtcaga atgcgttgtc
    25321 acaagctaga aactttaaag aagtaacaaa tgttacaggt attgtattaa cgaaattaga
    25381 tggtacagcc aaaggtggta tcgtattagc cattcgtaat gaattgcaca tcccagttaa
    25441 atatgtaggt ttaggtgagc aattagatga cttacaacca tttaaccctg aaagttatgt
    25501 ctacggctta ttcgctgata tgattgaaca aaatgaagaa ataacaacag ttgaaaatga
    25561 tcaaattgta acagaagaaa aggacgataa tcatgggtca aaatgattta gttaaaacgt
    25621 tacgaatgaa ttatttgttt gatttttatc aatccttatt gacgaataaa caacgtaatt
    25681 atttggaatt attttatctt gaagattatt ctttaagtga aatcgcagat acttttaatg
    25741 tgagtagaca agcagtttat gataatataa gaagaactgg cgatttagtt gaagattatg
    25801 aaaagaaatt ggaattatac cagaaatttg agcaacgccg agaaatatat gatgaaatga
    25861 aacaacattt aagtaatcca gaacaaatac aacgttatat tcaacaatta gaagacttag
    25921 aatagatata aattgaggga ggacatcgat atggcatttg aagggttatc agaacgcttg
    25981 caagcgacga tgcaaaaaat gcgtggtaag ggtaaactta ctgaagctga tataaagata
    26041 atgatgcgtg aagtaagatt agcgttactt gaggctgacg taaactttaa agtggtaaaa
    26101 gaatttatta aaacagtatc agaacgcgca ttaggttccg atgtaatgca atcattaaca
    26161 ccagggcaac aagttattaa aatagttcaa gatgaattaa cgcagttgat gggtggagaa
    26221 aatacgtcga ttaatatgtc aaataaacca cctactgttg ttatgatggt tggtttacaa
    26281 ggtgctggta aaacaacaac tgcaggtaaa ttagcattat tgatgcgtaa aaaatacaac
    26341 aaaaaaccta tgttagttgc agcagatatt tatcgtccag cagcgataaa tcaattacaa
    26401 acagtaggga aacaaattga tattcctgta tacagtgaag gagatcaagt aaagccacaa
    26461 caaattgtaa ctaatgcatt aaaacatgct aaagaagaac atttagactt tgtaatcatt
    26521 gatacagcag gtcgattaca catcgatgaa gcattgatga acgaattaaa agaagtaaaa
    26581 gacattgcta aaccaaacga aattatgtta gttgtcgatt caatgacggg tcaagatgct
    26641 gtcaatgttg cagaatcttt tgacgatcaa cttgatgtca caggtgttac cttaactaaa
    26701 ttagatggtg atacacgtgg tggtgcagct ttatctattc gttcggtgac acaaaaacca
    26761 attaaatttg ttggtatgag tgaaaagtta gatggtttag agctattcca tcctgaacgt
    26821 atggcatcac gtattttagg tatgggtgat gtgttaagtt taattgaaaa agcgcaacaa
    26881 gatgtggatc aagaaaaagc aaaagattta gagaaaaaga tgcgtgagtc atcgtttact
    26941 ttagatgatt ttttagaaca acttgatcag gtgaaaaatc taggaccact ggatgatatt
    27001 atgaaaatga ttccaggtat gaataaaatg aaagggctag ataagcttaa tatgagtgaa
    27061 aagcaaattg atcatattaa agcgattatc cagtcaatga cgccggctga aagaaacaat
    27121 ccagacacat tgaatgtatc acgtaaaaag cgtattgcta aagggtctgg tcgttcatta
    27181 caagaagtca atcgtttgat gaaacaattt aacgatatga agaaaatgat gaaacagttc
    27241 actggtggcg gtaaaggtaa aaaaggtaaa cgcaatcaaa tgcaaaatat gttaaaaggt
    27301 atgaatttac cgttttaatg tttaatataa cacgctgatt tttgttgcaa tgagatgtgc
    27361 atcccgaaag ttatactaaa gaatataact ttatggggtg catattttta gatacacaat
    27421 aataattaaa aactgaaatt gctgatttgc tagaacatga ggttattgtt gctaatatta
    27481 ataagttata ttgggggaac gtgtgcggta gaagtggtac cattcatgtc cttacgagat
    27541 aaattaactt aacacgtagg tggtgaaatt tactatgaat ttttataacc tattaacagt
    27601 ctaatttagt aatcacatag ttattattgt aagaaaaagg ttcaataatt ttcctggtaa
    27661 agaaaaaact ctttacaaac attcatacac ctgttaatat tatttcttgt agaaaataaa
    27721 aattaaaaca tgacttaaag gagattttat aaatggcagt taaaattcgt ttaacacgtt
    27781 taggttcaaa aagaaatcca ttctatcgta tcgtagtagc agatgctcgt tctccacgtg
    27841 acggacgtat catcgaacaa atcggtactt ataacccaac gagcgctaat gctccagaaa
    27901 ttaaagttga cgaagcgtta gctttaaaat ggttaaatga tggtgcgaaa ccaactgata
    27961 cagttcacaa tatcttatca aaagaaggta ttatgaaaaa atttgacgaa caaaagaaag
    28021 ctaagtaatt tagcgtaaaa ttgttctaac aataagaata actcgtttac actgacagtt
    28081 attactcaat gatacgttgg gaatatcaca tgttagtaat atagaacgta ttgggtacca
    28141 taatggtgcc ctttttcttt gaattatttt caattaaaat agaagtgttc aaagcataga
    28201 gttggaggta atagaatgag agttgaagtt ggtcaaattg ttaacacaca tggtattaaa
    28261 ggtgaaatta aagtaaaatc caattcagac tttacagacg ttcgttttca acccggtcaa
    28321 gtgctgacag ttgtgcataa caataacgac cttgaatata ctgttaagtc acatagagtg
    28381 cataaagggc ttcatatgct tacatttgaa ggtattaata atattaatga tattgagcat
    28441 ttaaaaggga gttctattta tcaagagcgt gatcatgaag atatcgtact tgaggaaaat
    28501 gaattttatt attcagatat tataggatgt acagtttttg atgatcaaga aacaccaata
    28561 ggtcgtgtaa ttaatatatt tgaaacaggt gcgaatgatg tgtgggtgat taaaggatct
    28621 aaagaatatt tgattcctta tattgctgat gttgtaaaag aagtggatgt tgaaaataaa
    28681 aaaattatca tcacgccaat ggaaggattg ttggattaat gaaaattgat tatttaactt
    28741 tatttcctga aatgtttgat ggtgttttaa atcattcaat tatgaaacgt gcccaagaaa
    28801 acaataaatt acaaatcaat acggttaatt ttagagatta tgcaattaac aagcacaacc
    28861 aagtagatga ttatccgtat ggtggcggac aaggtatggt gttaaagcct gaacctgttt
    28921 ttaatgcgat ggaagactta gatgtcacag aacaaacacg cgttatttta atgtgtccac
    28981 aaggcgagcc attttcacat cagaaagctg ttgaattaag caaggccgac cacatcgttt
    29041 tcatatgcgg acattatgaa ggttacgatg aacgtatccg aacacatctt gtcacagatg
    29101 aaatatcaat gggtgactat gttttaactg gtggagaatt gccagcgatg accatgactg
    29161 atgctattgt tagactgatt ccaggtgttt taggtaatga acagtcacat caagacgatt
    29221 cattttcaga tgggttatta gagtttccgc aatatacacg tccgcgtgaa tttaagggtc
    29281 taacagttcc agatgtttta ttgtctggaa atcatgccaa tattgatgca tggagacatg
    29341 agcaaaagtt gatccgcaca tataataaaa gacctgactt aattgaaaaa tatccattaa
    29401 ctaatgcaga taagcaaata ttagaaagat ataaaatagg attgaaaaaa ggttagtatt
    29461 gtgctaacat aatctgagtg tggaaacact aaaatcacta tgatccgctg ctatatattt
    29521 gtcgaggcaa gaacataggt tgaagaggag aatttataat gacaaatcac aaattaatcg
    29581 aagcagtaac taaatcacaa ttacgtacag acttaccaag tttccgtcct ggtgatactt
    29641 tacgtgtaca cgtacgtatc attgagggta ctcgtgagcg tatccaagta ttcgaaggcg
    29701 ttgtaattaa acgtcgtggc ggtggcgttt ctgaaacgtt tacagttcgt aaaatttcat
    29761 caggtgttgg cgtggaacgt acattcccat tacacacacc aaaaattgaa aaaatcgaag
    29821 ttaaacgtcg tggtaaagta cgtcgtgcta aattatatta cttacgtagt ttacgtggta
    29881 aagctgctag aatccaagaa attcgttaat cagcatttaa acaaagctat gtatgagtca
    29941 aattcgactc aaacaataaa gccatctagg tcacttttat ggagtgatat agatggcttt
    30001 ttttgctgtt tagttaatgt aaagttgata atgtaattgt ttattccttc tctattcata
    30061 acgtgtttgc tcaaaatggt tatataaaca aaaatctact aagtgccatt gaaaaggact
    30121 tagtagattt attaaatata tgcgtagcaa cattattttt gtcttgccca tctcgtgaaa
    30181 ataatactac atataatgcc aaatatagta attgttataa gtaaataata gtatggtggg
    30241 gtatagctca attgaatctt tgttatattt ttaggtgctt taatgccggt cataacacca
    30301 tttacttgtt caactttaag actttgatca cctgatgtcg ctttcatacc ttgattatat
    30361 gctgttggca aaacaatata cccagatgaa tttttatttt tagtaatagt ataaccatgc
    30421 tttgtcttac taactttgac agcttctaat gaatttgaag cgtctttaag cgtggtataa
    30481 tcttcgccgt atatcccttt taaatttact cgatacttac ctttaggcaa tgataatcta
    30541 attctatctg gagctttaat gcgtatcgtt acgggtgtta caacgcgtcg atatttataa
    30601 gtgagtttat ttctttcttg tgtatattca ttcactttaa catcatgagc tttatccggc
    30661 gaaagtaatt ctaaatccat ttcaaaatac aaatctttaa attgattaga aactgatttt
    30721 ggcaactgta cagttagacc accattattt tgtttaactt gtaataaatg ttttgtagga
    30781 gattgccagg ctgcactatt taatttaatt gttgaatctg atagtaaatt tttattggct
    30841 ttaaaatgtg tattaacatc tttaatattg ttagaaacaa tcccttgcaa cattgcttgt
    30901 tctttatcta atggagattt taattcttta ttggaaaaga ccttatttgt aatatgtgca
    30961 cttggataat ggatggtatt tttagaatga atccaacgaa ctttattgtc tttgtgttca
    31021 gacttaattt taaatccata tggtaagttg tcatcatgat tcactctaat tcgatcatta
    31081 acattccaaa gtgatagtaa attttgacga ttgccaagta atctataagt gctgttttta
    31141 tcgattggca tattaatttg gagtgtcttg tcataatatt ttaaaatgtc tccattaaaa
    31201 atactagaat ataatgaaat gccattataa tgatatataa atggtgaatt taatgcatag
    31261 tctgacatat aatcaatgcg attaaatgag cctgttgcat tttgatttat cttttttata
    31321 agctggttta catagttact atggtaatca tgttgtttca acgttgataa tgattgttga
    31381 taaggtttga ttgccatgtt tttgttgtta tctaaaatga cgatttgttg aatcataacg
    31441 attaatacta atattgcaac ggttaatttt ttataacgcc ataaactaaa ttttaaaata
    31501 acggcaagca ctattagcag gataatacct actataagtg caagtgggtg tgtcggtgat
    31561 agtaatacat aaagtattgc gatgatgcat acgggtattg ttctgattaa ataatatttc
    31621 atatttaatg ttgataaatg ttgaataaac aatccgcaaa gagcacttga tgatagtgct
    31681 aagatataca cccaacgcct ttctggaaat gaaaaaccat taaaagcact gtcgaaatac
    31741 tgtgataatg aacctataaa taatatccat gttactattg cgaaaagtct ataaaagtaa
    31801 aaacgataca gtttgaatga caataatgca acgatagtaa gaattgaaat cgtaatataa
    31861 aatccatcgc taaagaaaaa ataatgataa tcaagtggtg tcaaaaacgg tatatcaaca
    31921 ttgggatttt gctttctgtc attttccaaa aacgcagaaa tgccagtgaa taaaccaaat
    31981 acacttgata acacactcaa aactgtagca gatatgacgc aaattaattt ttgtgttcta
    32041 gagacaatgt catatttgta agtgaaaatg agtcgatata aatagtagca acctataata
    32101 atagcttgat aataactgaa ataaaaattg ctaaatagtg ttaaggctat cgcaacaatg
    32161 aaaataccga ttttgcgttg ttgaaaatat ctttccaaac caagaatcga taatggcaat
    32221 aaatataata aatttccata aaatgaccaa gtaaaattaa agtatataac gacagttgac
    32281 atgccgtata aaatcgtagc gatcatattt gctgagcgtt taaagtgtaa tattttaaat
    32341 aagtagaagg tcacgacaaa tgttatgata gctcgtatca tagccataat aagttggttt
    32401 gtcggccaaa aatgtattgt cgtcggatta aatataccaa ccgtttctcc tattttaatg
    32461 aatagaaaat ttagccacat taaaggtgac agcgaataat aatatgatag tcctttcata
    32521 taatcgccac ctaatccaaa cgatgcatca tataaactag aaaaactacg tagatgttca
    32581 tacaaataca tttgaaatgg catcatttga cggaatccat ctccagcccc gctaaaaaca
    32641 gtaccattca caatataatc atagatatga gtagaaaata aaataagcgt taatattaca
    32701 ctaatgaaag ttataacaaa gaattgtttg acgtttgaat ttagccactt ttttaacaca
    32761 acattatcct caactttcaa atttaaaatt aagtttaact gaaactaaag ttaatgaggt
    32821 tcttgatagg taaagacgaa gatgactgtg gaacagatac cttatcatag ttacttaaac
    32881 tttggatcat tttcagttta tcattaaaca aatatattga ataataaaaa tgtcatactg
    32941 ataaagatga atgtcactta ataagtaact tagatttaac aaatgatgat ttttaattgt
    33001 agaaaacttg aaataatcac ttatacctaa atctaaagca ttgttaagaa gtgtgacaat
    33061 gttaaaataa atatagttga attaatgaat ttgttctata attaacaggt tatagatttt
    33121 aataatgaga aaagaattga cgaaagtaag gtgaattgaa tggttattca atggtatcca
    33181 ggacatatgg cgaaagccaa aagagaagta agtgaacaat taaaaaaagt agatgtagtg
    33241 tttgaactag tagatgcaag aattccatat agttcaagaa accctatgat agatgaagtt
    33301 attaaccaaa aaccacgtgt tgttatatta aataaaaaag atatgtctaa tttaaatgag
    33361 atgtcaaaat gggaacaatt ttttattgat aaaggatact atcctgtatc agtggatgct
    33421 aagcacggta aaaatttaaa gaaagtggaa gctgcagcaa ttaaggcgac tgctgaaaaa
    33481 tttgaacgcg aaaaagcgaa aggacttaaa cctagagcga taagagcaat gatcgttgga
    33541 attccaaatg ttggtaaatc cacattaata aataaactgg caaagcgtag tattgcgcag
    33601 actggtaata aaccaggtgt gaccaaacaa caacaatgga ttaaagttgg taatgcatta
    33661 caactattag acacaccagg gatactttgg cctaaatttg aagatgaaga agtcggtaag
    33721 aagttgagtt taactggtgc gataaaagat agtattgtgc acttagatga agttgccatc
    33781 tatggattaa actttttaat tcaaaatgat ttagcgcgat taaagtcaca ttataatatt
    33841 gaagttcctg aagatgcaga aatcatagcg tggtttgatg cgatagggaa aaaacgtggc
    33901 ttaattcgac gtggtaatga aattgattac gaagcagtca ttgaactgat tatttatgat
    33961 attcgaaatg ctaaaatagg aaattattgt tttgatattt ttaaagatat gactgaggaa
    34021 ttagcaaatg acgctaacaa ttaaagaagt tacgcagttg attaatgcgg ttaatacaat
    34081 agaagaatta gaaaatcatg aatgcttttt agatgagcga aaaggtgttc aaaatgccat
    34141 agctaggcgc agaaaagcgt tagaaaaaga acaagcttta aaagaaaagt atgttgaaat
    34201 gacttacttt gaaaatgaaa tattaaaaga gcatcctaat gctattattt gtgggattga
    34261 tgaagttgga agaggacctt tagcaggtcc agtcgttgca tgcgcaacaa ttttaaattc
    34321 aaatcacaat tatttgggcc ttgatgactc gaaaaaagta cctgttacga aacgtctaga
    34381 attaaatgaa gcactaaaaa atgaagttac tgcttttgca tatgggatcg cgacagctga
    34441 agaaatagat gaatttaata tttataaagc tactcaaatc gccatgcagc gagctattga
    34501 tggattatca gtacaaccaa cgcatttatt gatagacgcg atgacgcttg ataatgcact
    34561 gcctcaagta tctttaatca agggtgatgc aagaagtgtg tccattgcag ctgcaagtat
    34621 catggcaaag gtttttcgtg atgattatat gacacagtta tctaaagatt atcctgaata
    34681 tggttttgaa aaaaacgcgg gttacggtac caaacaacat ttactagcaa tcgatgatat
    34741 tggcattatg aaagagcata gaaaaagctt tgaacctata aaatcgttac tgtaaatcgt
    34801 aaaattcaat taaatgataa aaaagtagca tttaagttta caatagcgct tacatttttt
    34861 ataattgtaa gggaacttaa cctaagtaac aggaggatgg aagatgaata tccacgagta
    34921 tcaaggtaaa gaaatatttc gttcaatggg cgttgcagtt ccagaaggac gagtagcatt
    34981 tactgctgaa gaagcggtgg agaaagcaaa agaattaaat tctgatgttt atgttgtaaa
    35041 agcacaaatt catgctggag gtagaggtaa agcaggcgga gtaaaaattg ctaaatcttt
    35101 atctgaggta gaaacatatg caaaagaatt attagggaaa actttggtga cacatcaaac
    35161 tggtccagaa ggtaaagaaa ttaagcgttt atatatcgaa gaaggttgtg ctattcaaaa
    35221 agaatattac gttggattcg ttattgatcg tgcgactgac caagtaacat tgatggcgtc
    35281 tgaagaaggg ggcactgaga ttgaagaagt tgctgcgaag actcctgaaa agatcttcaa
    35341 agaaactatc gatccagtaa tcggactttc accattccaa gcaagacgaa ttgcgtttaa
    35401 tattaatatt cctaaagaat ctgttaacaa agctgctaaa ttcttattag cactttataa
    35461 tgtattcatt gaaaaagatt gttcaatcgt agaaatcaac ccattagtta caacagctga
    35521 tggtgatgta ttggcattag atgctaaaat taattttgat gataatgcat tattcagaca
    35581 taaagatgtt gtagaattac gtgatttaga agaagaagat ccgaaagaga ttgaagcgtc
    35641 taaacatgat ttatcataca ttgcattaga tggtgacatc ggatgtatgg ttaatggtgc
    35701 aggtttagcc atggcaacaa tggatacgat taatcatttc ggtggaaacc cagccaattt
    35761 cttagatgca ggcggaagcg ctactagaga aaaagtaact gaagcattta aaatcatttt
    35821 aggtgatgaa aatgttaaag gtatttttgt aaacattttc ggtggcatta tgaaatgtga
    35881 tgttatcgca gaaggtatcg ttgaagctgt aaaagaagta gatttaactt taccactagt
    35941 tgtacgccta gaaggtacaa atgttgagtt aggtaaaaaa atcttaaaag actcaggatt
    36001 agcaattgaa ccagcagcaa caatggctga aggtgcacaa aaaattgtta aactagtcaa
    36061 agaagcataa ggaaaggatg ggagcactaa gatgagtgta tttatagata agaatactaa
    36121 agtaatggta caaggtatta cagggtctac tgcccttttc catacaaaac aaatgcttga
    36181 ttatggtacg aaaatagtag caggtgtgac gcctggtaaa ggtggtcaag ttgttgaagg
    36241 cgttcctgtt ttcaacactg ttgaagaagc taaaaatgaa actggggcaa cggtttcagt
    36301 catttacgtt ccagcaccat ttgctgcaga ctcaatttta gaagcagctg atgcagactt
    36361 agatatggtt atttgtatca ctgaacatat tcctgtatta gacatggtta aagttaaacg
    36421 ctacttacaa ggtagaaaaa cacgtttagt tggtccgaac tgtccaggtg tgattacagc
    36481 agatgaatgt aaaattggta ttatgcctgg ctatattcac aaaaaaggtc atgttggtgt
    36541 agtatctcgt tcaggtacat taacatatga agcagtgcac caattgactg aagaaggtat
    36601 tggtcaaact acagctgttg gtattggtgg agacccagtc aacggaacaa actttattga
    36661 tgttttaaaa gcattcaatg aagatgacga aacgaaagca gttgttatga ttggtgaaat
    36721 cggtggtacg gctgaagaag aagcagctga atggattaaa gcgaatatga caaaaccagt
    36781 tgtaggcttt atcggtggac aaacagcacc tcctggaaaa cgtatgggac atgctggtgc
    36841 aatcatttca ggtggtaaag gtactgctga agagaaaatt aaaacattaa atagttgtgg
    36901 tgtgaaaaca gcggcaacac cttcagaaat tggttcaaca ttaattgaag ctgctaaaga
    36961 agcaggtatt tatgaatcat tattaactgt taataaataa agttaaaaga tgatataaat
    37021 ggctatagcc cattccatac tttataaaag tatcggaaat gggctatttg tcgtgcgatg
    37081 aattttagta cacttttcat attttggaaa gacaagttaa ttattttacc tcctacaata
    37141 gctttataaa acaaagaaaa ttttaattaa aatatatagc gcactatgtt ataatgtttg
    37201 tatattattg taaggagtgt ttcatcatga ataaacaaca aagtaaagta cgctattcaa
    37261 ttagaaaagt tagtattgga attttgtcaa tttcaatagg tatgtttttg gcattgggta
    37321 tgtcgaacaa agcatatgca gatgaaattg ataaatctaa agattttaca agagggtatg
    37381 agcaaaatgt attcgcgaaa tcagagttaa atgctaataa aaatacgaca aaagacaaaa
    37441 taaaaaatga aggtgctgtt aaaacatcgg acacaagttt aaagttagac aacaaatcag
    37501 caatttcaaa cggaaatgaa attaatcaag atataaagat ttcaaatact ccgaaaaact
    37561 caagccaagg taacaatcta gttattaata acaatgaact tactaaagaa attaaaattg
    37621 caaacttgga agctcaaaat tctaatcaga agaaaacgaa taaagttact aataattact
    37681 ttggttacta cagttttaga gaagctccaa aaacacaaat ctatactgta aaaaaaggag
    37741 acacacttag tgctatagca ttaaaataca aaactacagt ttcaaatatt caaaatacaa
    37801 ataatatagc aaatcctaat ttaatattta ttggtcaaaa attaaaagtg ccaatgacac
    37861 cattagtaga accaaaacca aaaacagtgt cttcaaataa taaaagtaat agtaatagca
    37921 gtacattaaa ttatttgaaa acattagaga atagaggatg ggatttcgac ggtagttatg
    37981 gatggcaatg tttcgattta gttaatgtat attggaatca tctttatggt catggattaa
    38041 aaggatatgg agctaaagat ataccatatg caaataattt taatagtgaa gctaaaattt
    38101 atcacaacac accaactttc aaagctgaac ctggggactt agtggttttt agtggaagat
    38161 ttggtggagg atatggtcat acagctattg tcttaaatgg tgattatgat ggaaaattaa
    38221 tgaagttcca aagtttagat caaaactgga ataatggtgg atggcgtaaa gcagaggttg
    38281 cacataaagt tgttcataat tatgaaaatg atatgatttt tattagacca tttaaaaaag
    38341 cataatttaa atcgaaggca ggacattgaa atatgaaatt ttcaacttta agtgaagaag
    38401 aatttaccaa ctacaccaaa aagcacttca aacattatac acagtctata gaattatata
    38461 attatagaaa taaaataaat catgaagcac atattgtggg agtgaagaat gataaaaatg
    38521 aagttatagc tgcatgttta ttaacagagg cacgaatttt taaattctac aaatatttct
    38581 actctcatag aggtccttta cttgattatt tcgatgctaa attagtttgt tactttttta
    38641 aagaattatc taaattcatt tataaaaata gaggagtatt tattcttgtt gatccatatt
    38701 taatagagaa tttaagagat gcaaatggta ggataataaa gaattataat aattcagtga
    38761 tagtaaagat gctagggaaa attgggtatc tccatcaagg ttatacaaca ggatattcaa
    38821 ataaaagtca aattaggtgg atttctgtat tggatttaaa agataaagat gagaatcaac
    38881 ttttaaaaga aatggaatac caaactagaa gaaatataaa aaagactatt gagattggtg
    38941 ttaaggttga agatttatct attgaagaaa caaatcgatt ttataaattg tttcaaatgg
    39001 ctgaagaaaa acatggtttt catttcatga atgaagatta ttttaaacga atgcaagaaa
    39061 tatataaaga taaggcaatg ttaaagatag cttgtataaa tcttaatgaa tatcaagata
    39121 aattaaaaat acaattattg aaaatcgaaa atgaaatgat gactgtgaac agagcattaa
    39181 atgaaaatcc aaattctaaa aaaaataaat caaaattaaa tcagttaaat a
//
LOCUS       DALAGD010000007        39195 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.007, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000007 DALAGD010000000
VERSION     DALAGD010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 39195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39195
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.007"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(55..128)
                     /locus_tag="O2701_000276"
     tRNA            complement(55..128)
                     /locus_tag="O2701_000276"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(94..96),aa:Gly,seq:tcc)
     gene            complement(151..233)
                     /locus_tag="O2701_000277"
     tRNA            complement(151..233)
                     /locus_tag="O2701_000277"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(198..200),aa:Leu,seq:gag)
     gene            complement(880..1335)
                     /locus_tag="O2701_000278"
     CDS             complement(880..1335)
                     /locus_tag="O2701_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HCZ4292192.1"
                     /translation="MNNDKLQRMVENLSEEKFGRTFRHCAYFNKRLRTTGGRYLLKSH
                     DIEINPKQYEHYGEDAVVKIILHELCHYHLHIAGKGYQHKDQDFKRLSQQVGAPRFCN
                     SIESYQQRANYEYYCTKCHAKYIRIRKVDTNRMRCGHCNGKLRMKRQLK"
     gene            complement(1328..3478)
                     /locus_tag="O2701_000279"
     CDS             complement(1328..3478)
                     /locus_tag="O2701_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCZ4292193.1"
                     /translation="MDNQLINSIIEKYQFSKKQIEAVLTLLEEKNTVPFIARYRKEQT
                     GGLDEVQIKQIDDEYQYMVNLQKRKEEVIKNIEQQGLLTEELKKDILKQNKLQRVEDL
                     YRPFKQKKKTRATEAKRKGLEPLAIWMKARKHEVSIEEKAQQFINEEVQSVEDAIKGA
                     QDIIAEQISDNPKYRTKILKDMYHQGVLTTSKKKNAEDEKGIFEMYYAYSEPIKRIAN
                     HRVLAVNRGEKEKVLSVKFEFDTTSVEDFIARQEINHNNVNRSYILEAIKDSLKRLIV
                     PSIEREIHADLTEKAENHAIDVFSENLRNLLLQPPMKGKQILGVDPAFRTGCKLAVIN
                     PFGTFIAKGVIYPHPPVSKKEAAEKDFVQMVKAYDVQLIAIGNGTASRETEQFVADLI
                     KKHQLPVQFIIVNEAGASVYSASEIARDEFPDFQVEERSAVSIGRRVQDPLSELVKID
                     PKSIGVGQYQHDVNQKALENALTFVVETAVNQVGVDVNTASSSLLQYVSGLSSQIAKN
                     IIAYREENGAIKHNKELSKIKRLGAKTFEQSIGFLRIVDGSEPLDNTSIHPESYKVTY
                     QLLDKLGFGGNDLGSDALKAKLNSLDMDELAIELQVGVPTLEDIIKSLKAPNRDPRDE
                     FETPILKSDVLSIEDLQEGMKLSGTVRNVVDFGAFVDIGVKQDGLVHVSKLSKKFVKN
                     PMDIVSVGDIVDVWIYSIDKNKDKVSLTMIDPHE"
     gene            complement(3912..4682)
                     /gene="sigB"
                     /locus_tag="O2701_000280"
     CDS             complement(3912..4682)
                     /gene="sigB"
                     /locus_tag="O2701_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigB"
                     /protein_id="HCZ4292194.1"
                     /translation="MAKESKSANEISPEQINQWIKEHQENKNTDAQDKLVKHYQKLIE
                     SLAYKYSKGQSHHEDLVQVGMVGLIGAINRFDMSFERKFEAFLVPTVIGEIKRYLRDK
                     TWSVHVPRRIKEIGPRIKKVSDELTAELERSPSISEIADRLEVSEEEVLEAMEMGQSY
                     NALSVDHSIEADKDGSTVTLLDIMGQQDDHYDLTEKRMILEKILPILSDREREIIQCT
                     FIEGLSQKETGERIGLSQMHVSRLQRTAIKKLQEAAHQ"
     gene            complement(4657..5136)
                     /gene="rsbW"
                     /locus_tag="O2701_000281"
     CDS             complement(4657..5136)
                     /gene="rsbW"
                     /locus_tag="O2701_000281"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001190828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma B factor RsbW"
                     /protein_id="HCZ4292195.1"
                     /translation="MQSKEDFIEMRVPASAEYVSLIRLTLSGVFSRAGATYDDIEDAK
                     IAVSEAVTNAVKHAYKENNNVGIINIYFEILEDKIKIVISDKGDSFDYETTKSKIGPY
                     DKDENIDFLREGGLGLFLIESLMDEVTVYKESGVTISMTKYIKKEQVRNNGERVEIS"
     gene            complement(5138..5464)
                     /locus_tag="O2701_000282"
     CDS             complement(5138..5464)
                     /locus_tag="O2701_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HCZ4292196.1"
                     /translation="MNLNIETTTQDKFYEVKVGGELDVYTVPELEEVLTPMRQDGTRD
                     IYVNLENVSYMDSTGLGLFVGTLKALNQNDKELYILGVSDRIGRLFEITGLKDLMHVN
                     EGTEVE"
     gene            complement(5583..6584)
                     /locus_tag="O2701_000283"
     CDS             complement(5583..6584)
                     /locus_tag="O2701_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HCZ4292197.1"
                     /translation="MEEFKQHYKGLIDESLTCQDKVELIKKCEKYTDEVIRKDVLPED
                     IVDIHKNYILTLNLTREDVFKTLDVLQEIVKGFGYSYRDYQRLVDKLQVHDKEIDLAS
                     SLQQTMLKTDIPQFDSIQIGVISVAAQKVSGDYFNLIDHNDGTMSFAVADVIGKGIPA
                     ALAMSMIKFGMDSYGHSQLPSDGLKRLNRVVEKNINQNMFVTMFYGLYEEMNHLLYCS
                     SAGHEPGYIYRAEKEEFEEISVRGRVLGISSQTRYQQQEIPIYLDDLIIILTDGVTEA
                     RNSEGTFIDKQKLLEYIKKHKHMHPQDIVQIIYEAILKLQNPNKKDDMTILIIKRVN"
     gene            complement(6934..7296)
                     /locus_tag="O2701_000284"
     CDS             complement(6934..7296)
                     /locus_tag="O2701_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCZ4292198.1"
                     /translation="MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVA
                     AITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIRTLDKKRLKEKLTYLSDDKMKEV
                     DNALMISLGLNAVAHQKN"
     gene            complement(7293..7463)
                     /gene="mazE"
                     /locus_tag="O2701_000285"
     CDS             complement(7293..7463)
                     /gene="mazE"
                     /locus_tag="O2701_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HCZ4292199.1"
                     /translation="MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDC
                     NETYLSSNSTNE"
     gene            complement(7548..8696)
                     /gene="alr"
                     /locus_tag="O2701_000286"
     CDS             complement(7548..8696)
                     /gene="alr"
                     /locus_tag="O2701_000286"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCZ4292200.1"
                     /translation="MSDKYYRSAYMNVDLNAVASNFKVFSTLHPNKTVMAVVKANAYG
                     LGSVKVARHLMENGATFFAVATLDEAIELRMHGITAKILVLGVLPAKDIDKAIQHRVA
                     LTVPSKQWLKEAIKNISGEQEKKLWLHIKLDTGMGRLGIKDTKTYQEVIEIIQQYEQL
                     VFEGVFTHFACADEPGDMTTEQYQRFKDMVNEAIKPEYIHCQNSAGSLLMDCQFCNAI
                     RPGISLYGYYPSEYVQQKVKVHLKPSVQLIANVVQTKTLQAGESVSYGATYTATDPTT
                     IALLPIGYADGYLRIMQGSFVNVNGHQCEVIGRVCMDQTIVKVPDQVKAGDSVILIDN
                     HRESPQSVEVVAEKQHTINYEVLCNLSRRLPRIYHDGDQRFVTNELLK"
     gene            complement(8762..9121)
                     /locus_tag="O2701_000287"
     CDS             complement(8762..9121)
                     /locus_tag="O2701_000287"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCZ4292201.1"
                     /translation="MIHGIGVDLIEIDRIQALYSKQPKLVERILTKNEQHKFNNFTHE
                     QRKIEFLAGRFATKEAFSKALGTGLGKHVAFNDIDCYNDELGKPKIDYEGFIVHVSIS
                     HTEHYAMSQVVLEKSAF"
     gene            complement(9125..9616)
                     /locus_tag="O2701_000288"
     CDS             complement(9125..9616)
                     /locus_tag="O2701_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCZ4292202.1"
                     /translation="MRDYNFMSPHAKKVMRLSAVLFWIPIALVLLIAFNILNWLFWEI
                     LDNHLSIVSSVIIILLVAVFTLVIAPVYRYKHCRYAFEKHHLRVRNGILFLDEKVIPY
                     FRIQNIDIDVGPIMRRYRLATLTLYTAGGQAEIELIDRSEARRLKRWLNNERHLENNK
                     NEE"
     gene            complement(9603..11186)
                     /locus_tag="O2701_000289"
     CDS             complement(9603..11186)
                     /locus_tag="O2701_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCZ4292203.1"
                     /translation="MSEPQKLHPISYFNGIINAIKQNIVVFFIFIIFQLKDFDYTNPE
                     SYLWIGIVFVFFLISYISQIVGIMNTRYWIEDNYFILTTGIFNKKRKELNIKRIQSVD
                     MTQGVVNQIIGGVDLQIKTPSDGIVLSVISKKQGEYLERYIDQLQTELKTEVAQKSSA
                     EKNDNCNHDIEGNVEDIAEGRLDEVQNTDSMLNQKNKRIPIYQMNFKESLFMAMTSGA
                     IGVTLAALVPIYGAVRELIPWSKLNHEISQWVQIVSISVLIIILLVLIVAYIIGTIIT
                     MIRFYGFSVMLENEQLKIEYGLFNKKKVTVPTKRLQAVVERQSYIRRLFGYTAIHFII
                     TSDFENKKIEDDDNDSGSVPILPFIKRDKAYQIIQNLVPDLSYQQVNEGMPLSGFHRH
                     FLIPSIIVIAMAGIGCYFWNQLLLLFTIITIVIIGLFVIKGYLYVKHAGFKMTEEQIT
                     IQNFELLKRNIYYFKAHHIIGFDTWQHTLLKRNNLNNFSFIVAKGVGAQSINLKYARS
                     EDANKLKSWYLRGENDEGL"
     gene            complement(11179..11658)
                     /locus_tag="O2701_000290"
     CDS             complement(11179..11658)
                     /locus_tag="O2701_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292204.1"
                     /translation="MSDYTKLTKSPKSALLYYYITNGLEFILSVAVYVIFYFIWLRFE
                     WPHYLLYILLVLGALSTLKLFIKPLWQYHCRFYKVDQLSVQYRTSFFIYKEETSRIER
                     LQYLSIKSNPISKVLNLYKVGFMTAGHTIYLPMMSHDDVKIIEERTMSNLRGVESDV"
     gene            complement(11866..12426)
                     /locus_tag="O2701_000291"
     CDS             complement(11866..12426)
                     /locus_tag="O2701_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCZ4292205.1"
                     /translation="MNTIRNSICLTIITMVLCGFLFPLAITLIGQIFFYQQANGSLIT
                     YDNRIVGSKLIGQHWTETRYFHGRPSAVDYNMNPEKLYKNGVSSGGSNESNGNTELIA
                     RMKHHVKFGNSNVTIDAATSSGSGLDPHITVENALKQAPRIADARHVSTSRVADLIQH
                     RKQRGVLTNDYVNVLELNIALDKMKD"
     gene            complement(12446..14473)
                     /gene="kdpB"
                     /locus_tag="O2701_000292"
     CDS             complement(12446..14473)
                     /gene="kdpB"
                     /locus_tag="O2701_000292"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCZ4292206.1"
                     /translation="MHHVNKYFNQTMVIEALKMSFYKLNLKQLIKNPIMFVVEVGMIL
                     TLILICFPDIFGTSYLSRGYLITIFIILLITILFANFSEAFAEGRGKAQADSLRQAQS
                     NLTARLIEENGAYRIVNATELKAGQNIRVENGETIPADGVVINGLATVDESAITGESA
                     PVIKESGGDFDGVIGGTLVTSDWLEIRVESEAGTSFLDKMIALVEGAERNKTPNEIAL
                     FTLLTTLTIIFLVVIVTLYPIASYLHLILPIAMLIALTVCLIPTTIGGLLSAIGIAGM
                     DRVTQFNVLAKSGRAVEVCGDVDVMILDKTGTITYGNRIASEFLPVNQQMLEKLIVAA
                     YMSSIYDDTPEGKSIVRLAKQMYINELPKDIDGTYKPFTAETRMSGIITNEISVFKGA
                     PNSMINLVKQQQGNIPLNIESLCMDVSSKGGTPLIVIENNVMLGVIYLKDVIKDGLVE
                     RFTELRKMGIETVMCTGDNALTAATIAKEAGVDRFVAECKPEDKIKVIKDEQAKGHIV
                     AMTGDGTNDAPALAQANIGLAMNSGTISAKEAANLIDLDSNPTKLIEVVKIGKQLLMT
                     RGALTTFSLANDVAKYFAILPALMMSTIPEMTSLNIMHLSSPKSAIISALIFNALIIV
                     ALIPIAMKGVKVKGYSIDRIFINNMLIYGLGGLIVPFLGIKLIDMIVQFFV"
     gene            complement(14492..16168)
                     /gene="kdpA"
                     /locus_tag="O2701_000293"
     CDS             complement(14492..16168)
                     /gene="kdpA"
                     /locus_tag="O2701_000293"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit A"
                     /protein_id="HCZ4292207.1"
                     /translation="MEIILFLTMMVMITYVFSGYLYRVALVQSSRVDLIFTRFENMCF
                     KIIGTDLEHMSAKTYVKHFLAFNGFMGFITFVLLIVQQWLFLNPNHNLNQSIDLAFNT
                     AISFLTNSNLQHYNGESDVTYLTQMIVMTYLMFTSSASGYAVCIAMLRRLTGLTNIIG
                     NFYQDIVRFIVRVLLPLSCLISILLMTQGVPQTLHANLMIRTLSGHIQHIAFGPIASL
                     ESIKHLGTNGGGFLAGNSATPFENPNIWSNFIEMGSMMLLPMSMLFLFGRMLSRHGKR
                     VHRHALILFVAMFFIFIAILTLTMWSEYRGNPILANLGIYGPNMEGKEVRFGAGLSAL
                     FTVITTAFTTGSVNNMHDSLTPIGGLGPMVLMMLNVVFGGEGVGLMNLLIFVLLTVFI
                     CSLMVGKTPEYLNMPIGAREMKCIVLVFLIHPILILVFSALAFMIPGASESITNPSFH
                     GISQVMYEMTSAAANNGSGFEGLKDDTTFWNISTGIIMLLSRYIPIILQLMIASSLVN
                     KKSYHQDKYTIAIDKPYFGVSLIVFIVLLSGLTFIPVLLLGPIGEFLTLK"
     gene            complement(16192..16302)
                     /gene="kdpF"
                     /locus_tag="O2701_000294"
     CDS             complement(16192..16302)
                     /gene="kdpF"
                     /locus_tag="O2701_000294"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCZ4292208.1"
                     /translation="MLLCCKEVMAMLIVLLLVVIALVLYLFYALIRSEKF"
     gene            16440..19097
                     /locus_tag="O2701_000295"
     CDS             16440..19097
                     /locus_tag="O2701_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HCZ4292209.1"
                     /translation="MSNTESLNIGKKRGSLTIYIGYSPGVGKTFEMLSNAIELFQSNV
                     DIKIGYIEPHQRDETNALAEQLPKITTNFTKHGSHHFQYLDVDRIIEESPTIVLIDEL
                     AHTNISRDRHEKRYMDIEEILNHGIDVHTTLNIQHIESLSSQIELMTGVHVKERVPDY
                     FIMSADVLEVVDISPEQLIKRLKAGKVYKKDRLDVAFSNFFTYAHLSELRTLTLRTVA
                     DLMSDKEKVRHNHKTSLKPHIAVAISGSIYNEAVIKEAFHIAQKEHAKFTAIYIDVFE
                     KNRQYKDSQKQVHQHLMLAKSLGAKVKVVYSQTVALGLDEWCKNQDVTKLIIGQHIRN
                     KWRDFFNTPLIDHLMSFEHSYKIEIVPIKQIPVELKMNKSPYRPKGKRFAIDMLKMIL
                     IQIICVMMGLWIYQLDKHESSTIILMIFLIGIILLSIWTRSFIIGFLAAIINVFVFNY
                     FFTEPRYTFEVYRFDYPITFIVSILTSILTSALLKQIKFQYSITKKQLYRTDLLFQFN
                     DSIKQTYTVENLLINAGYQINQLLQQSITIYVINQSKVIKTIPLQNHIDNTTQQHEQA
                     LSWVIKNERQAGATTDTFPGINKWLIPIGTSPIKGILAIDYQSSQVINPYDASILESM
                     LNELSLAVENVTLLKQTRESMLQAERQLTHSNFLRSISHDIRTPLTTIMGNLDILVSH
                     SKDMSIIEKEQLLVHSFQESQYLYLLVTNILSLTKLQSSNVQIKLQPYLVSELVEEID
                     MILERRHLKKRITVSSSVNLQFIHIDSKLILQALFNLIENAVKHTSTDTKINLSIRYA
                     SYEQIEFAVIDEGPGISLEEQQKIFEPFYTGSNKYFKDNQKESMGLGLYLVQTILHKH
                     QSNLQYKPNQPHGSIFYFNIYTDFNEGDV"
     gene            19097..19792
                     /locus_tag="O2701_000296"
     CDS             19097..19792
                     /locus_tag="O2701_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCZ4292210.1"
                     /translation="MQSKILIIEDDHAITHLLDVALTLDYYNVTTADNATQAHFKIQI
                     DKPDVILLDLGLPDKDGLCLISEIRQHTDIPIIVISARQEEQTIIQALDNGANDYMTK
                     PFNVDELRARIRVIERIAKSHQETNIVFTNGLLSIDFGSKSVVINNQEVHLTPNEFSL
                     LELLSNHKGKVLTYEMILKRIYGYVNKTEMPSLRVHMTSLRQKLSQCHEDAKDIIKTH
                     PRIGYQMLQWKEK"
     gene            complement(20161..21681)
                     /locus_tag="O2701_000297"
     CDS             complement(20161..21681)
                     /locus_tag="O2701_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCZ4292211.1"
                     /translation="MQNFKELGISDNTVQSLESMGFKEPTPIQKDSIPYALQGIDILG
                     QAQTGTGKTGAFGIPLIEKVVGKQGVQSLILAPTRELAMQVAEQLREFSRGQGVQVVT
                     VFGGMPIERQIKALKKGPQIVVGTPGRVIDHLNRRTLKTDGIHTLILDEADEMMNMGF
                     IDDMRFIMDKIPAVQRQTMLFSATMPKAIQALVQQFMKSPKIIKTMNNEMSDPQIEEF
                     YTIVKELEKFDTFTNFLDVHQPELAIVFGRTKRRVDELTSALISKGYKAEGLHGDITQ
                     AKRLEVLKKFKNDQINILVATDVAARGLDISGVSHVYNFDIPQDTESYTHRIGRTGRA
                     GKEGIAVTFVNPIEMDYIRQIEDANGRKMSALRPPHRKEVLQAREDDIKEKVENWMSK
                     ESESRLKRISTELLNEYNDVDLVAALLQELVEANDEVEVQLTFEKPLSRKGRNGKPSG
                     SRNRNSKRGNPKFDSKSKRSKGYSSKKKSTKKFDRKEKSSGGSRPMKGRTFADHQK"
     gene            complement(22198..23556)
                     /locus_tag="O2701_000298"
     CDS             complement(22198..23556)
                     /locus_tag="O2701_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCZ4292212.1"
                     /translation="MINVTLKQIQSWIPCEIEDQFLNQEINGVTIDSRAISKNMLFIP
                     FKGENVDGHRFVSKALQDGAGAAFYQRGTPIDENVSGPIIWVEDTLTALQQLAQAYLR
                     HVNPKVIAVTGSNGKTTTKDMIESVLHTEFKVKKTQGNYNNEIGLPLTILELDNDTEI
                     SILEMGMSGFHEIEFLSNLAQPDIAVITNIGESHMQDLGSREGIAKAKSEITIGLKDN
                     GTFIYDGDEPLLKPHVKEVENAKCISIGVATDNALVCSVDDRDTTGISFTINNKEHYD
                     LPILGKHNMKNATIAIAVGHELGLIYNTIYQNLKNVSLTGMRMEQHTLENDITVINDA
                     YNASPTSMRAAIDTLSTLTGRRILILGDVLELGENSKEMHIGVGNYLEEKHIDVLYTF
                     GNEAKYIYDSGQQHVEKAQHFNSKDDMIEVLINDLKAHDRVLVKGSRGMKLEEVVNAL
                     IS"
     gene            complement(23571..24641)
                     /locus_tag="O2701_000299"
     CDS             complement(23571..24641)
                     /locus_tag="O2701_000299"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCZ4292213.1"
                     /translation="MTKENICIVFGGKSAEHEVSILTAQNVLNAIDKDKYHVDIIYIT
                     NDGDWRKQNNITAEIKSTDELHLENGEALEISQLLKESSSGQPYDAVFPLLHGPNGED
                     GTIQGLFEVLDVPYVGNGVLSAASSMDKLVMKQLFEHRGLPQLPYISFLRSEYEKYEH
                     NILKLVNDKLNYPVFVKPANLGSSVGISKCNNEAELKEGIKEAFQFDRKLVIEQGVNA
                     REIEVAVLGNDYPEATWPGEVVKDVAFYDYKSKYKDGKVQLQIPADLDEDVQLTLRNM
                     ALEAFKATDCSGLVRADFFVTEDNQIYINETNAMPGFTAFSMYPKLWENMGLSYPELI
                     TKLIELAKERHQDKQKNKYKID"
     gene            24959..26161
                     /locus_tag="O2701_000300"
     CDS             24959..26161
                     /locus_tag="O2701_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HCZ4292214.1"
                     /translation="MNYSSRQQPDKHWLRKVDWVLVATIAVLAIFSVLLINSAMGGGQ
                     YSANFGIRQIFYYILGAIFAGIIMFISPKKIKHYTYLLYFLICLLLIGLLVIPESPIT
                     PIINGAKSWYTFGPISIQPSEFMKIILILALARVVSRHNQFTFNKSFQSDLLLFFKII
                     GVSLVPSILILLQNDLGTTLVLAAIIAGVMLVSGITWRILAPIFITGIVGAMTVILGI
                     LYAPALIENLLGVQLYQMGRINSWLDPYTYSSGDGYHLTESLKAIGSGQLLGKGYNHG
                     EVYIPENHTDFIFSVIGEELGFIGSVILILIFLFLIFHLIRLAAKIEDQFNKIFIVGF
                     VTLLVFHILQNIGMTIQLLPITGIPLPFISYGGSALWSMMTGIGIVLSIYYHEPKRYV
                     DLYHPKSN"
     gene            complement(26425..26562)
                     /locus_tag="O2701_000301"
     CDS             complement(26425..26562)
                     /locus_tag="O2701_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292215.1"
                     /translation="MKRPEKIQNVVKLLSSLGVNIKKTKSRLDIINTLPASNKVSHEL
                     K"
     gene            complement(26690..26899)
                     /locus_tag="O2701_000302"
     CDS             complement(26690..26899)
                     /locus_tag="O2701_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HCZ4292216.1"
                     /translation="MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVT
                     VSYETPANLNSIEKEIYDEGYKIVF"
     gene            complement(26911..27204)
                     /gene="csoR"
                     /locus_tag="O2701_000303"
     CDS             complement(26911..27204)
                     /gene="csoR"
                     /locus_tag="O2701_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-sensing transcriptional repressor CsoR"
                     /protein_id="HCZ4292217.1"
                     /translation="MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDV
                     LTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQEEAMEELLVTFQKLIKD"
     gene            27370..28854
                     /gene="cls"
                     /locus_tag="O2701_000304"
     CDS             27370..28854
                     /gene="cls"
                     /locus_tag="O2701_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCZ4292218.1"
                     /translation="MIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANS
                     IWAWLLVLVFLPLFGFILYLLLGRQIQRDQIFKIDKEDKKGLELIVDEQLAALKNENF
                     SNSNYQIVKFKEMIQMLLYNNAAFLTTDNDLKIYTDGQEKFDDLIQDIRNATDYIHFQ
                     YYIIQNDELGRTILNELGKKAEQGVEVKILYDDMGSRGLRKKGLRPFRNKGGHAEAFF
                     PSKLPLINLRMNNRNHRKIVVIDGQIGYVGGFNVGDEYLGKSKKFGYWRDTHLRIVGD
                     AVNALQLRFILDWNSQATRDHISYDDRYFPDVNSGGTIGVQIASSGPDEEWEQIKYGY
                     LKMISSAKKSIYIQSPYFIPDQAFLDSIKIAALGGVDVNIMIPNKPDHPFVFWATLKN
                     AASLLDAGVKVFHYDNGFLHSKTLVIDDEIASVGTANMDHRSFTLNFEVNAFIYDQQI
                     AKKLKQAFIDDLAVSSELTKARYAKRSLWIKFKEGISQLLSPIL"
     gene            28882..29529
                     /locus_tag="O2701_000305"
     CDS             28882..29529
                     /locus_tag="O2701_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCZ4292219.1"
                     /translation="MQQSDVISAAKKYMESIHQNDYTGHDIAHVYRVTALAKSIAENE
                     GVNDTLVIELACLLHDTVDEKVVDANKQYVELKSFLSSLSLSTEDQEHILFIINNMSY
                     RNGKNDHVTLSLEGQIVRDADRLDAIGAIGVARTFQFAGHFGEPMWTEHMSLDKINDD
                     LVEQLPPSAIKHFFEKLLKLESLMHTDTAKMIAKERHDFMMMYLKQFFTEWNCHD"
     gene            complement(30342..31214)
                     /gene="yidC"
                     /locus_tag="O2701_000306"
     CDS             complement(30342..31214)
                     /gene="yidC"
                     /locus_tag="O2701_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCZ4292220.1"
                     /translation="MKKKALLPLFLGIMVFLAGCDYSKPEKRSGFFYNTFVDPMKNVL
                     DWLGNNLLNDNYGLAIIILVLVIRIILLPFMLSNYKNSHMMRQKMKVAKPEVEKIQEK
                     VKRARTQEEKMAANQELMQVYKKYDMNPIKSMLGCLPMLIQLPIIMGLYFVLKDQLVD
                     GLFKYPHFLWFDLGRPDIWITIIAGVLYFIQAYVSSKTMPDEQRQMGYMMMVISPIMI
                     IWISLSSASALGLYWSVSAAFLVVQTHFANIYYEKVAKKEVQPFIEAYEREHNGGSNK
                     KGKNTQVVSKKKKK"
     gene            complement(31300..31941)
                     /locus_tag="O2701_000307"
     CDS             complement(31300..31941)
                     /locus_tag="O2701_000307"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCZ4292221.1"
                     /translation="MFNQSYLNVYFICGTSDVPSHRTIHEVLEAALKAGITLFQFREK
                     GESALKGNDKLVLAKELQHLCHQYDVPFIVNDDVSLAKEINADGIHVGQDDAKVKEIA
                     QYFTDKIIGLSISDLDEYAKSDLTHVDYIGVGPIYPTPSKHDAHIPVGPEMIATFKEM
                     NPQLPIVAIGGINTNNVAPIVEAGANGISVISAISKSENIEKTVNRFKDFFNN"
     gene            complement(31943..32734)
                     /gene="thiM"
                     /locus_tag="O2701_000308"
     CDS             complement(31943..32734)
                     /gene="thiM"
                     /locus_tag="O2701_000308"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCZ4292222.1"
                     /translation="MNYLNKIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPE
                     EAEEFYKVAQALLINIGTLTAENEQDIIAIAQTANEAGLPIVFDPVAVGASTYRKQFC
                     KLLLKSAKVSVIKGNASEILALIDDTATMKGTDSDANLDAVAIAKKAYATYKTAIVIT
                     GKEDVIVQDNKAFVLANGSPLLARVTGAGCLLGGVIAGFLFRETEPDIEALIEAVSVF
                     NIAAEVAAENENCGGPGTFSPLLLDTLYHLNETTYQQRIRIQEVE"
     gene            complement(32718..33548)
                     /gene="thiD"
                     /locus_tag="O2701_000309"
     CDS             complement(32718..33548)
                     /gene="thiD"
                     /locus_tag="O2701_000309"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCZ4292223.1"
                     /translation="MIKPKIALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVA
                     QNTLGVQHIHNLNHQWVDEQLDSVFNDTLPHAIKTGMIATADTMETIRHYLMQHESIP
                     YVIDPVMLAKSGDSLMDNDTKQNLQHTLLPLADVVTPNLPEAEEITGLTIDSEEKIMQ
                     AGRIFINEIGSKGVIIKGGHSNDTDIAKDYLFTNEGVQTFENERFKTKHTHGTGCTFS
                     AVITAELAKGRPLFEAVHKAKKFISMSIQYTPEIGRGRGPVNHFAYLKKEGLDDELSK
                     "
     gene            complement(33541..34230)
                     /gene="tenA"
                     /locus_tag="O2701_000310"
     CDS             complement(33541..34230)
                     /gene="tenA"
                     /locus_tag="O2701_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HCZ4292224.1"
                     /translation="MEFSQKLYQAAKPIINDIYEDDFIQKMLSGDIGADALRHYLKAD
                     AAYLKEFTNLYALLIPKMNSMNDVKFLVEQIEFMVEGEVLAHDILAQIVGESYEEIIK
                     TKVWPPSGDHYIKHMYFQAHSRENAIYTIAAMAPCPYIYAELAKRSQSDHKLNREKDT
                     AKWFDFYSTEMDDIINVFEALMNKLAESMSDKELEQVKQVFLESCIHERRFFNMAMTL
                     EQWEFGGKVND"
     regulatory      complement(34310..34412)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(34616..35311)
                     /gene="sceD"
                     /locus_tag="O2701_000311"
     CDS             complement(34616..35311)
                     /gene="sceD"
                     /locus_tag="O2701_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase SceD"
                     /protein_id="HCZ4292225.1"
                     /translation="MKKTLLASSLAVGLGIVAGNAGHEAHASEADLNKASLAQMAQSN
                     DQTLNQKPIEAGAYNYTFDYEGFTYHFESDGTHFAWNYHATGTNGADMSAQAPATNNV
                     APSAVQANQVQSQEVEAPQNAQTQQPQASTSNNSQVTATPTESKSSEGSSVNVNAHLK
                     QIAQRESGGNIHAVNPTSGAAGKYQFLQSTWDSVAPAKYKGVSPANAPESVQDAAAVK
                     LYNTGGAGHWVTA"
     gene            complement(35748..36095)
                     /locus_tag="O2701_000312"
     CDS             complement(35748..36095)
                     /locus_tag="O2701_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002466750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCZ4292226.1"
                     /translation="MLNKIVIVGRLTKDAQIFEKEDRKIATFCVATHRNYKDENGEIV
                     CDYLFCKAFGKLASNIEKYTNQGTLVGITGQMRSRKYDKDGQTHFVTELYVETIKFMS
                     PKSQNNEILSDSI"
     gene            36289..36729
                     /locus_tag="O2701_000313"
     CDS             36289..36729
                     /locus_tag="O2701_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwpF-like family protein"
                     /protein_id="HCZ4292227.1"
                     /translation="MKTFKAVRFQIVNEHGRIIEYELEDGVIINKEESGTGWLLEIVI
                     SNEHYETFKEYQDNEQLLDIRVVITRPANDPALFESTVKSIKNFKTTMSIVFECHIYT
                     LRQQYAESLLEQLIDDGLSGEELKKSFNRMMQSKPKLKDEKLEE"
     gene            complement(36787..37227)
                     /gene="fabZ"
                     /locus_tag="O2701_000314"
     CDS             complement(36787..37227)
                     /gene="fabZ"
                     /locus_tag="O2701_000314"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCZ4292228.1"
                     /translation="METIFDYNQIKQIIPHRQPFLLIDKVVEYEEGQRCVAIKQVSGN
                     EPFFQGHFPEYAVMPGVLITEALAQTGAVAILNSEENKGKIALFAGIDKCRFKRQVVP
                     GDTLTLEVEITKIKGPIGKGNAKATVDGQLACSCELTFAIQDVK"
     gene            complement(37261..38526)
                     /gene="murA"
                     /locus_tag="O2701_000315"
     CDS             complement(37261..38526)
                     /gene="murA"
                     /locus_tag="O2701_000315"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCZ4292229.1"
                     /translation="MDKIVIKGGNKLTGEVKVEGAKNAVLPILTASLLASDKPSKLVN
                     VPALSDVETINNVLTTLNADVTYKKDENAVVVDATKTLNEEAPYEYVSKMRASILVMG
                     PLLARLGHAIVALPGGCAIGSRPIEQHIKGFEALGAEIHLENGNIYANAKDGLKGTSI
                     HLDFPSVGATQNIIMAASLAKGKTLIENAAKEPEIVDLANYINEMGGRITGAGTDTIT
                     INGVESLHGVEHAIIPDRIEAGTLLIAGAITRGDIFVRDAIKEHMASLVYKLEEMGVE
                     LDYQEDGIRVRAEGELQPVDIKTLPHPGFPTDMQSQMMALLLTANGHKVVTETVFENR
                     FMHVAEFKRMNANINVEGRSAKLEGKSQLQGAQVKATDLRAAAALILAGLVADGKTSV
                     TELTHLDRGYVDLHGKLKQLGADIERIND"
     gene            complement(38637..38870)
                     /locus_tag="O2701_000316"
     CDS             complement(38637..38870)
                     /locus_tag="O2701_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HCZ4292230.1"
                     /translation="MDYIGQYAVIHLVLHVVCICIAYWALQSIRLDQFFKKGYATQLQ
                     VCMIFVAILLGTAVSNFIVDLLQYSTQVKYLIK"
     gene            complement(39002..39094)
                     /locus_tag="O2701_000317"
     CDS             complement(39002..39094)
                     /locus_tag="O2701_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292231.1"
                     /translation="MLKSKFPSPLSTVDISFFEIIYIFSYQDIL"
BASE COUNT        12476 a         7185 c         5659 g        13875 t
ORIGIN      
        1 gaattaacgt tgacatattg tcattcagtt ttcaatgttc attaaaatta tctatggagc
       61 gggtgatggg aatcgaaccc acaacatcag cttggaaggc tgaggttttg ccattaaact
      121 acacccgctt atttaaatta agaattttga tgcggccgag aggacttgaa cctccacggg
      181 atttctccca ctaggccctc aacctagcgc gtctgccatt ccgccacgac cgcggatata
      241 ggcatttaaa acatcttcgt ttttcagaac gttatttatt atattatgtt taaaaatatt
      301 tgtcaataac ttttaatcgt ttttttacat taaattaatt tgtgttatca gttgttaaca
      361 caacgctatt tataataaaa catttagaac ttattgtcaa catattttat aaattaaatt
      421 tagaattgac tgtctttcaa atcaaagttt atttaatttg ttctttttta aggaacgtgt
      481 ttaatcatac taactataag ctgtacagtc aatagaaaag ttcaatcaaa atgtcatgaa
      541 aaatgtataa ttatcttttg tgctacttta aagctttaat tgggtaaagt ttatatatta
      601 attattacta aaatacgcca agatttttaa tctaatcatt ttaatttcat cctatcaagc
      661 cacaatttat gccattacct atatcataag tagaaaagcc gagattgcga agtattaaat
      721 tacgacaatc tcggctttaa acattttata aacacaattt cttcttattc aattactatg
      781 tttaccaatc aaaataactc actgataaat tctatataaa ttccatacaa ctcataaata
      841 atcttacata tacacgaatt agctctattt gtattgtact tatttcaatt gtcttttcat
      901 tcttaatttt ccattgcaat gtccgcaacg catacgattc gtatcaactt tacggatacg
      961 tatatatttt gcatgacatt tagtgcaata atattcataa tttgcccgtt gttgatagct
     1021 ttcaatgctg ttacaaaatc taggcgctcc aacctgttgg ctcaatcgtt taaaatcttg
     1081 atctttatgt tgataacctt taccagcaat atgcaagtga taatgacaca attcgtgcag
     1141 tataattttt acaacagcat cttctccata atgctcatat tgttttggat taatttcaat
     1201 atcatgggac tttaaaagat aacgtccgcc tgttgtacgt aaccttttat taaaatatgc
     1261 acaatgtcga aacgtacgtc caaatttttc ttccgaaaga ttctcaacca ttcgctgaag
     1321 tttgtcatta ttcatgtgga tcaatcatcg ttaatgatac tttgtcttta tttttgtcaa
     1381 tactgtaaat ccaaacgtca acgatatcac caacactgac aatatccatt ggatttttta
     1441 cgaacttctt agaaagtttc gaaacatgga caagtccatc ttgtttaaca cctatatcta
     1501 caaatgctcc aaaatctact acatttctaa ctgtaccact caatttcatt ccttcttgta
     1561 aatcttcaat tgatagcaca tcggatttaa ggattggtgt ttcaaactcg tcccttggat
     1621 ctcgattagg tgctttcaag gatttaataa tatcctctaa tgtaggtaca ccgacttgta
     1681 attcaatcgc cagttcatcc atatccaatg aatttaattt agcttttaat gcatcactac
     1741 ctaaatcatt gccaccaaat ccgagtttat ctaataactg atacgttact ttatagcttt
     1801 cgggatgaat cgatgtatta tccaatggct ctgaaccgtc tactattctt aaaaatccta
     1861 tactctgctc aaaagtttta gcacccaaac gcttaatctt gcttaactct ttattatgct
     1921 ttattgcgcc attttcttct ctatatgcaa ttatattttt agcaatttgc gaacttagac
     1981 cagacacata ttgtaataat gatgatgatg ctgtattgac atcaacacct acttggttaa
     2041 ctgctgtttc aacaacgaaa gttaatgcat tttctagtgc tttttgatta acatcatgct
     2101 ggtattgtcc gacaccaata gatttaggat cgattttaac taattcactt aatggatctt
     2161 gaacgcgtcg tccaattgat acggcacttc tttcttccac ttgaaaatca ggaaattcat
     2221 ctctagcaat ttctgatgct gagtatactg aagcgcccgc ttcattgaca atgatgaatt
     2281 gtactggcaa ctgatgcttt ttaattaaat ctgcaacaaa ttgttctgtt tcacgacttg
     2341 cagtaccatt gccaattgca attaattgca catcatacgc tttaaccatt tgtacaaaat
     2401 ccttctctgc tgcctctttt ttagaaactg gtggatgcgg ataaatcaca ccttttgcta
     2461 taaaagtacc gaatgggtta atgactgcta atttacaacc tgttctaaat gctggatcta
     2521 cgcctaatat ttgtttacct ttcattggag gttgcagtaa tagatttctt aagttttcac
     2581 taaaaacatc tattgcatga ttttcagctt tttcagttaa atcagcatgg atttcacgct
     2641 ctatcgaagg gacaattaag cgtttcaagc tatctttaat cgcctctaaa atataactgc
     2701 gatttacatt attatgattg atttcttgac gtgcaatgaa atcctctact gatgtcgtat
     2761 cgaattcaaa ctttacagat aatactttct ctttttcacc acgattaaca gctaaaactc
     2821 tatgattagc aatgcgttta attggctcac tatatgcata gtacatttca aaaatacctt
     2881 tttcatcttc agcatttttc tttttagatg tagttaacac accttgatga tacatatctt
     2941 ttaaaatttt tgttctatat ttaggattat ctgaaatttg ttccgcaata atatcttgtg
     3001 cacctttgat agcatcttca accgattgca cttcttcatt tataaattgt tgtgcttttt
     3061 cttcaattga gacttcatgt ttacgtgcct tcatccatat cgctaatggc tctaaccctt
     3121 tacgtttcgc ctcagttgcc cttgtctttt tcttttgttt aaaaggccta tataggtctt
     3181 caacacgttg taatttgttc tgttttaaaa tatccttctt taattcctca gtaagtaatc
     3241 cttgctgttc tatatttttg ataacttctt ctttacgttt ttgtaaattg accatatatt
     3301 ggtattcgtc atcaatttgc tttatttgaa cttcatctag tccaccagtt tgctcttttc
     3361 gatacctcgc aataaatggt actgtatttt tttcttctag cagtgttaat actgcttcaa
     3421 tttgtttttt actaaattga tatttctcta tgattgaatt aatcaattga ttgtccattt
     3481 acttaaccca cactttttct taaatttact catttatttt aacatattct aaaatacttc
     3541 tattaaggta tgatacttaa tgtaattttc acttccaaaa catttaaaac gaatgattag
     3601 gtcatactat attttaaaac gcactaagat tattgaaaat gacacaccat catttctaaa
     3661 tttcataatc taagactact ttttttattc taatagcaac ccacctattt aaaacgacaa
     3721 ttagatatct tcttcactta aattattaat actatatcgc gacatttatg tggatacaca
     3781 ttaaactaca ctaataatgt attaattcga aaatcttaat taaattaaaa atacccctcg
     3841 atttcaattt aataatcaag ggataattat atggattttt cattataaaa cgtatcatta
     3901 ataaacaaat tctattgatg tgctgcttct tgtaatttct taattgccgt tctctgaagt
     3961 cgtgatacat gcatttgact taaaccgata cgctcacctg tctctttttg actcaatcct
     4021 tcaataaacg tacattgtat gatttctcgt tcgcgatcag ataatatagg taatattttt
     4081 tctaaaatca tacgtttttc agttaagtca taatggtcat cttgttgccc cataatatct
     4141 aatagcgtaa cagttgaacc atctttatca gcttcaatgg aatgatcaac acttaacgca
     4201 ttataacttt gtcccatttc cattgcttct aacacttctt cttctgagac ttctaatcga
     4261 tcagctattt cactgataga aggtgaacgc tctaattcag cggttagttc atcgctcact
     4321 tttttgattc ttggcccaat ttctttaata cgtctcggaa catgtacact ccaagtttta
     4381 tctcgtagat atcttttgat ttcaccgatt acagtaggta ctaaaaaggc ttcaaacttc
     4441 cgttcaaagg acatatcgaa tctatttatg gcacctatta aaccaaccat accaacttga
     4501 actaaatctt cgtgatgtga ttgtccttta gaatatttat atgccaatga ctcaattagt
     4561 ttttggtaat gtttaactaa cttatcctgt gcatctgtat tcttattttc ttggtgttct
     4621 ttaatccatt ggttaatttg ctcaggtgaa atttcattag ctgatttcga ctctttcgcc
     4681 attatttcgc acctgctctt tttttatata cttagtcata ctgattgtca caccagattc
     4741 tttatatact gtgacttcat ccattaaaga ttcgattaaa aataaaccta ggccaccttc
     4801 gcgtaaaaag tctatatttt cgtctttatc gtaaggacct atttttgatt tagttgtttc
     4861 ataatcaaaa ctgtcacctt tatcagaaat aacaatttta attttatctt ctaaaatttc
     4921 aaaatatatg ttaataatgc ccacattgtt attttctttg tatgcatgtt taactgcatt
     4981 tgtcacagct tcactaactg caatcttggc atcttcaata tcatcatatg tagcaccagc
     5041 tctcgaaaaa acgccagaaa gtgttaaacg aattaaactt acatactctg ccgatgctgg
     5101 cacgcgcatt tcgataaaat cttctttaga ttgcatgtta ttcgacctcc gttccttcat
     5161 taacatgcat taaatcctta agaccagtaa tttcaaatag tctaccgata cgatctgaca
     5221 cacctaaaat gtatagttct ttatcatttt ggtttaatgc ttttaatgta cctacgaata
     5281 aacctaaacc tgtcgaatcc atataactca cattttctaa attaacataa atatcacgag
     5341 ttccatcttg tctcataggt gttaaaacct cttctaattc aggcacagta taaacatcta
     5401 attctccacc gactttaact tcgtaaaatt tatcttgagt ggttgtttct atattaagat
     5461 tcatttcatt acactcctac ttattaaaaa tatttatctt cattttaagc gctgtatcca
     5521 ccatatttat atttcaaatt attatatacc catcgaaata atttctaatc ttctttttta
     5581 aattaattta ctctttttat aatcaaaata gtcatatcat cttttttatt tgggttttga
     5641 agctttaaaa ttgcttcata gataatttga acaatatctt gtgggtgcat atgtttatgt
     5701 tttttaatat attctaaaag tttttgttta tctataaagg taccttcact atttctagct
     5761 tcagtcacac catccgttaa aatgataatt aaatcatcaa ggtatattgg aatttcttgt
     5821 tgttgatatc gtgtttgtga actgattcct aacactctac ctctaactga aatttcttca
     5881 aattcttctt tttcagcgcg ataaatatat ccaggctcat gaccagctga actacaatac
     5941 aataaatggt tcatttcttc atataaacca taaaacattg tgacgaacat attttgatta
     6001 atattctttt caacaacacg atttaaacgt tttaaaccat cactcggtaa ttgtgagtgt
     6061 ccataagaat ccatgccaaa ctttatcata ctcattgcta aagcagctgg tatacctttt
     6121 ccaatgacat ctgcaacagc aaagctcatt gtgccatcgt tatggtcaat taaattaaaa
     6181 taatctccac ttactttttg tgccgccact gaaataacgc caatttgaat actatcaaat
     6241 tgtggaatat ctgttttaag cattgtttgt tgtaagctag aagctaagtc tatctcttta
     6301 tcgtgaactt gaagtttatc taccaatctt tgataatctc gataactata accaaagcct
     6361 ttaacgattt cttgtaagac atctaatgtc ttgaacacat cttcacgcgt taagtttaac
     6421 gtcaatatat agtttttgtg aatatcgaca atgtcttcag gcaagacgtc cttacgaatc
     6481 acttcgtcag tgtatttctc acactttttt atcaattcta ctttatcttg gcacgttaaa
     6541 ctttcatcaa ttaaaccctt ataatgttgc ttaaattctt ccacgattag ttgcctcctc
     6601 acttccctta caaagcaaat gcttaagcaa ggataattat gattcactta ctaacattta
     6661 tttaattgca cttatcaatt ttgtcgtttg caattttgct gtagatgata cttttaagct
     6721 aaaaaaaact ttaggatgga atacctaaag cctaaaaaac ttaagacatc cttttataat
     6781 aaaaaatatg aatatcataa ctggtatttg ctaaaatctt tgtttataag aaaattcttc
     6841 gtacagaatt tagaaatagt tataattatt ataaagttat tgtcttttat atcaatattt
     6901 tatttatctc tgaaaaatac atataataga cgcctaattt ttctggtgag ctactgcatt
     6961 cagccctaaa ctaatcatta gtgcattatc tacttctttc attttatcat cggataagta
     7021 cgtcagtttt tctttcaatc gttttttatc aagtgtacga atttgttcta ataatataac
     7081 tgagtcttta tccaacttat actttttctt ttcaatctct acatgtgtcg gtattttcgc
     7141 tttattaatc ctaccagtta ttgccgcaac aataactgta ggactatatt tattaccagt
     7201 atcattttga attatgacta caggtctgac tcccccttgt tcagatccct gtactggtga
     7261 taaatctgct aaataaacat ctcctcgtct aatcattcat tcgttgaatt agaagataaa
     7321 tatgtttcgt tgcaatcgca tgcttcacac tctatcggaa aagcttcgtt cgctagggag
     7381 agatttaaat cagccatttg tgaatatcct tcttttaaag attgttctaa gctatgactt
     7441 ctattttgac taaaagataa catatgagaa cctccaattg attccaaaag ttattaaatg
     7501 acctttaatt actctaatga taacaaattt tatatgcact gaccatatta ttttaacaat
     7561 tcatttgtta caaaacgttg atcaccatca tgatagattc gcggcaaacg tctcgacaag
     7621 ttacaaagca cttcataatt aatagtatgt tgcttttcag ctaccacctc taccgactgt
     7681 ggactttctc tatgattatc tattaaaatc accgaatctc cagctttaac ttgatctggc
     7741 actttaacaa ttgtctgatc catacatacg cgaccaataa cttcgcattg atgaccattt
     7801 acatttacaa agctaccttg cattatgcgt aaatagccat ctgcatatcc aataggtaac
     7861 aatgctattg tagttgggtc agtagctgta taagttgcac cataacttac agactcaccc
     7921 gcttgtagcg tctttgtttg aactacatta gcaattaatt gcacacttgg tttaaggtgt
     7981 actttaactt tttgctgtac atactctgat ggataatatc cataaaggga aattcctggt
     8041 cttattgcat tacagaattg gcaatccatt aatagagagc ctgctgagtt ctgacaatgt
     8101 atatattcag gtttaattgc ttcattgacc atatctttaa aacgttgata ttgttcagtt
     8161 gtcatatctc ctggttcgtc agcacaggca aagtgtgtaa acacgccttc aaatacaagt
     8221 tgctcatatt gttgaatgat ttcaatcact tcttgatacg ttttagtatc tttaatacct
     8281 aaacgtccca ttcctgtatc taatttaatg tgcaaccata actttttctc ttgctcacca
     8341 gaaatgtttt taattgcttc tttcaaccac tgtttagacg gaaccgttaa ggcaactcgg
     8401 tgttgtatcg ctttatcaat atctttagct ggtaacacac ctaagactaa aattttagca
     8461 gtaatcccat gcattctaag ttctatcgct tcatctaacg ttgctacagc aaaaaatgtg
     8521 gcgccatttt ccattaaatg acgtgctact ttaacactac ctagtccata ggcattggct
     8581 ttaacgacag ccatcactgt tttatttgga tgcaatgtac tgaatacttt gaaatttgat
     8641 gcaacagcgt ttaaatctac attcatatac gcagatctat aatatttatc cgacatatta
     8701 cttcctcctg taattcccac acgttttaaa actagatctt aattatcatt gtataacaaa
     8761 tttaaaatgc tgacttttct aaaacaactt ggctcatcgc ataatgctca gtgtgtgaga
     8821 tactaacatg tacgataaac ccttcgtaat caatctttgg tttgccaagt tcgtcgttgt
     8881 aacagtctat atcgttaaaa gctacatgtt ttcctaagcc tgtgcctaat gctttactga
     8941 acgcttcttt tgtagcaaac ctgccagcta aaaattcaat tttacgttgc tcatgtgtga
     9001 aattgttgaa tttgtgctgt tcatttttag ttaaaatccg ctcaaccaat tttggttgct
     9061 tactatataa cgcttgtatt cgatcgattt cgattaaatc tacaccaatt ccatgtatca
     9121 ttgattattc ctcattttta ttgttttcta aatgtctctc gttatttaac catcgtttca
     9181 atctacgtgc ttcacttcta tctattaatt caatttcagc ttgaccaccc gctgtataaa
     9241 gggtcaaagt tgctaatcga tagcgtctca ttataggacc aacatcgata tcaatatttt
     9301 gaatacgaaa atatggtatt accttttcat ccaaaaataa aatgccgttt cgtacacgca
     9361 aatggtgttt ttcaaatgca tatctgcagt gcttatatcg atagactggc gctataacaa
     9421 gcgtgaaaac agcaacaagt aatattataa tcacactact aacaatggat aaatggttat
     9481 ctaaaatctc ccaaaatagc cagttcaaaa tattaaatgc gattaaaagt acaagcgcta
     9541 tgggtatcca aaacagcaca gcacttaacc tcatcacttt tttagcgtgt ggtgacataa
     9601 aattataatc cctcatcatt ttcacctctt aaataccatg atttcaattt atttgcatct
     9661 tcacttcttg cgtattttaa gttaatcgat tgggctccca cacctttagc aacaataaag
     9721 ctaaaattat ttaaattgtt gcgtttaagt aatgtatgtt gccaagtgtc aaatcctata
     9781 atgtgatgcg ctttaaaata ataaatattt cgtttcaata gctcgaaatt ctggatagta
     9841 atttgttctt ctgtcatttt aaaacccgca tgtttgacat aaagatatcc tttgatcaca
     9901 aataaaccaa taatgactat tgttataatc gtaaataaca ataataattg attccaaaaa
     9961 taacagccta tacctgccat agctatgaca ataatactag gtattaaaaa gtgtctgtgg
    10021 aaacctgaca aaggcatacc ttcattaact tgttgataag ataaatctgg tactaaattc
    10081 tggataattt gatatgcttt gtctcgttta ataaacggca atatcggcac actacctgaa
    10141 tcattgtcat catcttcgat ttttttattt tcaaaatcac ttgtgatgat aaaatgtatt
    10201 gctgtgtagc caaataatct tcgtatatat gactgacgtt caacaacagc ttgcaatcgt
    10261 ttcgttggta cagttacttt cttcttgtta aagagaccat attcaatttt aagttgctca
    10321 ttttcaagca tcaccgaaaa gccataaaat cttatcattg ttataatcgt tccaataata
    10381 tatgccacta ttaatactag taaaatgatg attaatactg aaatacttac aatttgaacc
    10441 cattgactaa tttcatgatt tagcttcgac catgggatca actctcttac agccccgtaa
    10501 atcggtacta aagctgctaa cgttacacca atggcgccac tggtcattgc cataaatagt
    10561 gattctttaa aattcatctg atatatagga atgcgtttat ttttctgatt aagcatacta
    10621 tcagtgttct gcacttcatc taagcgacct tctgcgatgt cttccacatt accttcaatg
    10681 tcatgattac agttgtcatt cttctcagca ctagactttt gcgccacttc tgtcttcaac
    10741 tctgtttgca attgatcaat atatcgttca agatattcac cttgtttttt cgaaataaca
    10801 cttaagacaa taccatcact tggtgttttg atttgtaaat ccacaccacc aataatttga
    10861 ttgacgaccc cttgtgtcat atcaacggac tgaatacgtt ttatatttaa ttctttacgc
    10921 tttttattaa aaatacctgt tgttaaaatg aaataattat cctcaatcca atatcgcgtg
    10981 ttcataattc cgacaatttg agaaatgtat gatattaaaa agaatacaaa tacaatacct
    11041 atccataaat atgattcggg attcgtataa tcaaaatctt tcaattgaaa gataatgaaa
    11101 ataaaaaaga cgactatgtt ttgtttgata gcattgatta tgccattaaa atatgaaatc
    11161 ggatgtaatt tttgaggttc agacatcact ttcaacccct ctcaaattcg acatagttct
    11221 ctcttcgatt attttaacat cgtcatgaga catcatcggt aaataaatag tatgacctgc
    11281 agtcataaat ccaactttat acaaattaag cactttacta attggattag atttaatcga
    11341 caagtattgt aaacgttcaa ttcgactcgt ttcttcttta tatataaaaa atgatgtacg
    11401 atattgtaca cttagttgat caactttata aaagcgacaa tgatattgcc ataaaggctt
    11461 aataaataat tttaatgtac tcagagcacc taaaaccaac aaaatataaa gtaagtaatg
    11521 tggccattca aatcttaacc atataaaata aaaaatgaca tacacagcta cactcaatat
    11581 aaattctaag ccattcgtaa tgtagtaata caacaatgct gacttaggac tcttagtcaa
    11641 cttagtataa tctgacatat acccctctcc ccaaataaaa aattatacgg atttataatc
    11701 tatttcattt tatttttata tgatgataat tatagcatat ggaatatttc atgctaattt
    11761 attcttccta aaggtacatc taaaaattta attaagcaga aagtgcttga attgctaaaa
    11821 agacaccatg tataatttta tcaacatgat gcctttcatc tataatcaat ctttcatctt
    11881 atcaagagcg atatttagtt caagcacatt cacataatca tttgttaaca caccacgctg
    11941 cttacgatgt tgaatcaagt cggccactct tgaagtagat acatgacgag catcagcaat
    12001 acgaggtgct tgcttcaatg cattttcgac cgtaatatgc ggatctaagc ccgacccaga
    12061 acttgttgca gcatctattg ttacatttga attcccaaat ttaacatgat gtttcatgcg
    12121 tgctattaat tcggtgtttc cattcgattc attacttcca cctgaagata cgccgttttt
    12181 atataatttt tcaggattca tattataatc aactgcactc ggtctcccgt gaaaatatcg
    12241 tgtctctgtc cagtgctgtc caatcaattt tgatccaact atacgattgt catacgtaat
    12301 taaactgcca tttgcttgtt gataaaaaaa tatttgacca attaacgtga tagctaacgg
    12361 gaataaaaat ccacataata ccatagttat tatcgttaaa caaatactat ttcttatcgt
    12421 attcatggta caggctcctt cctctttaca caaaaaattg tacaatcata tctattaatt
    12481 taatgcctaa aaacgggacg attaatccac ctaatccata aatcaacata ttatttataa
    12541 agattctatc aatgctgtaa ccctttactt ttacaccttt catggcaatt ggaattaagg
    12601 caacaatgat taatgcattg aatatcaaag ctgatataat tgcagattta ggtgacgata
    12661 aatgcataat atttagtgaa gtcatttctg gaattgttga catcattagt gcaggtaaaa
    12721 ttgcaaagta ttttgctacg tcattagcca aactaaatgt cgttaatgca cctctcgtca
    12781 ttaataattg tttgcctatt tttacaacct ctattaactt tgtaggattc gaatctaaat
    12841 caattagatt agctgcctct ttagcactaa ttgtccctga gttcatagct aatcctatat
    12901 tcgcttgtgc tagcgcaggt gcatcatttg taccatctcc tgtcatcgca acaatatggc
    12961 ctttcgcttg ttcatctttg atgactttaa ttttatcttc gggtttacac tctgcaacaa
    13021 atctatcaac cccggcttct tttgcaattg tagctgctgt taaagcatta tcacctgtac
    13081 acataactgt ttcaatcccc atttttctca attcagtaaa tcgttctaca agaccatctt
    13141 taatcacatc ttttaaataa atcacgccaa gcatgacatt gttttcaatg actattaatg
    13201 gtgtgccacc tttactcgat acatccatac agagagactc aatattaaga ggaatattgc
    13261 cttgttgttg tttgacaaga tttatcatac tattaggtgc acctttgaat accgatattt
    13321 catttgtaat gattccgctc attctagttt cagctgtaaa aggcttatat gtgccatcaa
    13381 tgtctttagg cagctcattt atatacatct gcttcgctaa tcgtacaata ctttttcctt
    13441 ctggcgtatc atcgtagatt gatgacatat aagcagcgac tatcaatttt tcaagcattt
    13501 gttgattcac tggtaaaaat tcactagcga ttcgattgcc ataagtgatt gtgcctgtct
    13561 tgtctaaaat cattacatcg acatctccac atacttctac agcacgccca cttttcgcta
    13621 atacattgaa ttgagtaaca cgatccatgc ctgcaatacc aatcgccgat aacaaaccac
    13681 cgattgtcgt tggtattaaa catactgtta acgcaatgag catcgcaata ggtaaaatta
    13741 aatgcaggta agatgctatt ggatataacg ttacaataac gactaaaaat ataattgtta
    13801 acgttgttaa taatgtaaaa agtgcaattt catttggtgt tttatttctt tccgcccctt
    13861 caactaaggc aatcatttta tctaaaaaag atgtacccgc ttcactctca acacgtattt
    13921 ctaaccaatc agatgttaca agtgtaccgc caatgactcc atcaaaatcg ccacctgatt
    13981 cttttatcac aggtgcagac tcaccagtaa ttgcagattc atcaacggtt gctaatccat
    14041 ttattacaac gccatcagca gggattgttt ctccattttc tacccgaata ttttgtccgg
    14101 cttttaactc tgtggcgttc actatccgat acgcaccatt ttcttctatc aatcgagcag
    14161 ttaaatttga ttgtgcttgt cttaaactat cagcttgcgc ttttccacga ccttcagcaa
    14221 aggcttctga aaaattagca aacaatatag ttattaataa tatgataaaa attgtaatca
    14281 aataacctcg cgatagatag ctagttccaa atatgtcagg aaaacatatt aatatcaacg
    14341 ttaaaatcat tccaacctca acgacaaaca ttatcggatt ttttattaat tgtttaagat
    14401 tcagcttata aaaactcatt ttcaaagctt ctatcaccat tgtttgattg aaatatttat
    14461 ttacatgatg cattttttcc atccctttac tttattttaa agttaaaaat tcaccaatag
    14521 gaccaagtaa tagtactgga ataaatgtca aaccacttag taaaacgata aatacgatta
    14581 gtgatacgcc aaaataaggt ttatcaatcg ctattgtata tttatcttga tggtatgatt
    14641 ttttattcac taaacttgat gcaatcatta attgcaaaat aattggtata taacgagaaa
    14701 gcaacataat gattcctgta gagatattcc agaatgttgt atcatctttc agtccttcaa
    14761 accctgatcc attgttcgca gcagctgatg tcatttcata cataacttgt gaaataccat
    14821 gaaaagacgg attcgttata ctttcacttg ctccaggaat cataaaagca agtgctgaaa
    14881 atactaaaat taaaattggg tgtatgagaa agactaagac aatacatttc atttcacggg
    14941 cgccaattgg catatttaaa tattctggtg ttttaccaac catcaaactg catataaaca
    15001 ccgtcagtaa gacaaatatc aataaattca tgagtcctac gccttcgcca ccaaatacaa
    15061 catttagcat cattaatacc attggtccta atccacctat aggcgttaag ctatcatgca
    15121 tgttattaac agaacccgtt gtaaatgccg tcgtaataac tgtaaatagt gctgacaaac
    15181 ctgctccaaa ccgtacctct ttaccttcca tattcggtcc ataaatgcct aaattcgcta
    15241 gtattggatt accacgatac tcactccaca tagttaatgt aagaattgct ataaaaatga
    15301 aaaacattgc gacaaataat atcaacgcat gacgatgtac tcgtttacca tgtctactta
    15361 acatgcgacc aaataagaac aacattgaca taggaagtaa catcatactg cccatttcta
    15421 taaaattgct ccaaatattt ggattttcaa aaggtgtcgc agaatttcct gctaaaaatc
    15481 ctccaccatt cgtaccaaga tgttttattg attcaagtga tgcaataggt ccaaatgcaa
    15541 tatgttgaat atgtccgctt aaagtccgaa tcattaaatt agcatgcaac gtttgtggta
    15601 caccttgagt catcaataaa atactaatta aacatgataa tggtaaaagt actcggacaa
    15661 taaaccgaac aatatcttga taaaaattac caatgatatt agttaatcca gttaaacgtc
    15721 tcaacatcgc tatacaaacg gcgtaacctg atgcactaga tgtaaacatt aaatatgtca
    15781 ttacaatcat ttgcgttaaa tatgtcacat ctgattcacc gttatagtgt tgtaaattac
    15841 tatttgttaa aaaagatatt gctgtattaa acgctaaatc tatcgattgg tttaaattat
    15901 gatttggatt taaaaaaagc cattgctgaa ctattagcaa tacaaatgtt ataaacccca
    15961 taaatccatt aaatgccaga aaatgtttga catatgtttt agctgacatg tgttctaaat
    16021 ctgtgccgat aattttaaaa cacatatttt caaatctagt aaatattaaa tctactcttg
    16081 acgattgcac caatgctacg cgatatagat atccactaaa aacatacgta atcataacca
    16141 tcattgttag aaacaaaatt atttccatga taaccctcac ttaatatatt tctaaaattt
    16201 ttcactacga attaaggcat aaaataaata caaaactaat gcaataacta ccagtaataa
    16261 aacgatgagc attgccataa cctccttaca acacaacaac atcgtaacaa cttgtttatg
    16321 agagaaatat taattttcaa acttagttat taagaaatca ttaagatgtg tatgcagaaa
    16381 taaattttat agcatttaat tgtgaagaat attatgatat tgctatcgag gtgaaggtta
    16441 tgtcaaacac tgaatcgcta aacataggaa aaaagcgtgg atctttaaca atttacatcg
    16501 gttacagtcc aggtgttggt aaaacatttg agatgctttc aaatgccatt gaactatttc
    16561 aaagtaacgt tgatattaaa ataggatata ttgaaccgca tcagcgtgat gaaacaaatg
    16621 cattagctga acaattacct aaaatcacaa ccaattttac taaacatggt agtcatcatt
    16681 ttcaatattt agatgtcgac cgcataatcg aagaatcgcc gacaatagta cttatcgatg
    16741 agttagcaca tacgaatatt tctagagatc gtcatgagaa acgatatatg gatattgaag
    16801 aaattttaaa tcatggtatc gatgttcata ccactttgaa cattcaacat attgaaagtt
    16861 taagtagtca aattgaactg atgaccggtg tacatgttaa agaacgtgta cccgactatt
    16921 tcataatgag cgccgatgta ttagaagtcg tagatatctc acctgaacaa ttaattaaac
    16981 gcttaaaagc tggcaaggta tataaaaagg ataggctaga tgtagcattt agtaatttct
    17041 ttacgtatgc ccacctaagc gaactgcgta cattgacgtt aagaacagtt gccgacttga
    17101 tgagtgataa agaaaaagtc cgacacaacc ataaaacgtc actcaaacct catattgctg
    17161 tggcaattag tgggagcatt tataatgaag cagtaattaa agaggcattc catattgctc
    17221 aaaaagaaca tgcgaagttc actgctattt atatagatgt attcgaaaaa aacaggcaat
    17281 ataaagatag tcaaaagcaa gtgcatcaac atctcatgct tgcaaaatca ttaggagcaa
    17341 aagtaaaagt agtttatagc caaaccgttg cattaggatt agacgaatgg tgtaaaaatc
    17401 aagatgtaac caaattaatt atcggacaac atattagaaa taagtggcga gactttttca
    17461 atacaccttt aattgaccat ttaatgtcct ttgaacatag ctataaaatc gaaatcgttc
    17521 caatcaaaca aatacctgtt gaattgaaaa tgaacaaatc accctatcgt cctaaaggca
    17581 aacgtttcgc catagatatg ttaaaaatga ttttgattca aataatttgt gtaatgatgg
    17641 gactgtggat ttatcaactt gataagcatg agtctagtac gattatttta atgatttttc
    17701 tcatcggcat cattttatta tccatttgga cgcggtcctt catcattggc tttttagcag
    17761 caattattaa cgtatttgtt tttaattatt tttttacgga acctagatat acatttgaag
    17821 tatatcgctt tgactatcct attacattta tcgttagcat tttaacaagt attttaacga
    17881 gtgctttatt aaaacaaatt aaattccaat actccattac taaaaagcaa ctttatcgca
    17941 cagatttatt atttcaattt aatgattcga ttaaacaaac ttatacagtt gaaaacttac
    18001 taataaatgc tggataccaa attaatcaat tattgcaaca atcaattact atatacgtta
    18061 tcaatcaatc gaaagtaatt aaaacaatac cattgcaaaa ccatatcgat aatacgactc
    18121 aacagcatga acaagcatta agctgggtaa ttaaaaatga aagacaagct ggtgcaacaa
    18181 cagatacatt tccaggcatt aataaatggt tgataccaat tggcacatct ccgattaaag
    18241 gcattctagc cattgattac caaagttctc aagtgattaa tccatatgat gcatcaatat
    18301 tagaatcaat gttaaatgaa ctttctctcg ccgttgaaaa tgtgacgtta cttaaacaaa
    18361 cgagagaatc catgttacag gctgaacgtc aactgacaca ttcaaatttt ttaagatcaa
    18421 tttcgcatga catacgtaca ccgttaacaa caattatggg aaatttagat attttagtat
    18481 cacatagtaa agatatgtct atcatagaaa aggaacaatt acttgtacat agttttcaag
    18541 aaagtcagta tttatatcta ttagtgacaa atattttatc attaacaaaa ttacagtcat
    18601 caaatgttca gataaaattg caaccttatc tcgttagtga attagtagaa gaaatcgata
    18661 tgattttaga acgtcgccat ttaaaaaagc gtataactgt atcatcctca gtaaatttac
    18721 agtttataca cattgacagt aagttgattt tacaagcttt attcaatttg attgaaaatg
    18781 cagttaaaca cacttctact gacaccaaaa tcaatttatc tattcgttat gctagctatg
    18841 aacaaatcga atttgccgtt atagacgaag gacctggaat tagtttagaa gaacaacaaa
    18901 agatattcga gcctttttac acaggatcaa ataaatattt taaagataac caaaaagaaa
    18961 gcatgggact aggcttatat ttagtacaga ctatcctaca caaacatcaa tcaaacttac
    19021 aatataaacc gaaccaacca cacggcagta tattctattt caatatatat acagacttta
    19081 acgaaggaga cgtataatgc aatctaaaat attgataatt gaagatgatc acgcaatcac
    19141 acatttactt gatgttgcat taactttaga ttattacaat gtaactacag ccgacaatgc
    19201 cacacaagca cactttaaaa ttcaaattga taaaccagat gtcattttat tagatttagg
    19261 tttaccagat aaagatggat tatgtttgat ttcagaaatc aggcaacata ctgacattcc
    19321 tatcattgta ataagtgcaa gacaagaaga acaaacaatt attcaagctt tagataacgg
    19381 tgcgaatgac tatatgacta aaccttttaa tgttgatgag cttcgggcac gaatacgagt
    19441 catcgagaga atcgctaagt cacatcaaga aactaacatt gtatttacta acggtttgct
    19501 aagcatcgac tttggctcca aatctgttgt tattaataat caggaggtac atttgacgcc
    19561 aaatgaattt agcttgttag aactattgtc aaatcataaa ggaaaagtac tcacttatga
    19621 gatgatatta aaacgaatat atggctatgt taataagact gaaatgccta gtttaagagt
    19681 gcatatgaca tcattaagac aaaaactttc acaatgtcat gaggacgcaa aagatattat
    19741 taaaacacat ccacgcattg gttatcaaat gttgcagtgg aaagagaaat aattaaataa
    19801 aaaagatcgc tgccattaaa cgaatgattg gcagtgatct tttttatatt caacttatta
    19861 tttatcgcgc actagcgtca gcaaaaagcc ctattgtttt ttataagaac atgatactga
    19921 aatttcataa agtttattta tgttcgatta ttcttaattt aaaaatagtt ctttaaatta
    19981 tatactcacc acatttggtg gagaaccata tttacacaga atgatataac cgcatacaga
    20041 tctcaatcca attaaatatt taacctttaa aacgttagta ctttgatatc tacagagaat
    20101 caattattga aaacaaaaaa gagctcaaga cattacatct ttaagctctt aatctataaa
    20161 ttatttttga tggtcagcaa atgtgcgacc tttcataggt ctgcttccac cgctgctctt
    20221 ctctttacgg tcgaattttt ttgtactttt cttcttactt gagtatcctt ttgaacgttt
    20281 actcttactg tcaaatttag gattaccacg cttactattt ctgttacgag aaccacttgg
    20341 tttaccgtta cggcctttgc gagataatgg tttttcaaaa gttaattgaa cttcaacttc
    20401 atcgtttgct tctactaact cttgtaaaag tgcagcaact aaatcaacat cgttatattc
    20461 atttaacaac tctgtagaaa tgcgtttcaa gcgtgattct gactctttag acatccagtt
    20521 ttcaactttt tctttgatgt catcttcacg tgcttgaagt acttctttac gatgtggtgg
    20581 acgaagtgca ctcatttttc taccgtttgc atcttcaatt tgtctgatat aatccatttc
    20641 gattggatta acaaacgtta cagcgatacc ttctttacca gcacgacccg tacgaccaat
    20701 acggtgtgta tagctttcag tatcttgagg tatatcaaag ttataaacat gactcacacc
    20761 agaaatatct agtcctcttg ctgctacatc agtagcgact aaaatattaa tttggtcatt
    20821 tttaaatttc tttaatactt ctaaacgttt cgcttgtgta atatcaccat gtaaaccttc
    20881 agctttatat cctttagaaa tcaaagcact tgttaattca tcaacacgac gttttgtacg
    20941 tccgaatacg attgctaatt caggttgatg aacatctagg aaatttgtaa atgtatcaaa
    21001 tttctctaat tctttaacaa ttgtatagaa ttcttcgatt tgtggatcag acatttcatt
    21061 attcattgtc ttaatgattt ttggtgattt cataaattgt tgtactaaag cttggattgc
    21121 tttaggcatt gtagctgaga acaacattgt ttgacgttgt actgctggaa ttttatccat
    21181 aataaatctc atatcatcga tgaatcccat attcatcatt tcatcagctt catctaaaat
    21241 caaagtatga attccgtccg tttttaatgt gcgacgattt aaatggtcga taacacgccc
    21301 aggtgttccg actacgattt gtgggccttt tttcaaggct ttaatttggc gttcgatagg
    21361 cataccaccg aatacagtaa caacttggac accttgtcca cggctaaatt ctcttaattg
    21421 ttcagctacc tgcattgcca attctcttgt aggtgctaaa atcaacgatt gaaccccttg
    21481 tttccctact actttctcaa ttaaaggaat accgaatgct cctgttttac ctgtaccggt
    21541 ttgagcttgc ccaaggatat caattccttg taacgcataa gggatactgt ctttttggat
    21601 aggtgtcggc tctttaaatc ccattgattc aagtgactga accgtattat ccgaaatccc
    21661 tagttcttta aaattttgca aaataattct cctttttacc tgtttatttc tcaatatact
    21721 tttgcatata agttcattaa tatttcatca acttctatat agtaccacca aaaaaattta
    21781 atcgcaataa agcaatttgc gaatatttat tagaatcaca ttaattgatt gtcatatttt
    21841 tgtcaaaaag ctagcattag ataagatgta agcgttacgc atcattcgaa taatcattat
    21901 aaaaaatttt ttataaaata cgtatatcgt taacattcca caacaaattt tactgaaaca
    21961 catgtgaaat tagacattat aatactacat ctttttgtaa aacattatat aaacattcaa
    22021 actacttcct atataaagtg tgtacataga attaaccttg atttagtttc aaatttatag
    22081 atttaacata aaaagaaaaa ggcaattttg tatttacaca caaaaccacc tcttctttgt
    22141 aatcttttag tattaattca aatcattttt ataagtaaaa ggtccctcga ctaatctcta
    22201 tgaaattaaa gcatttacca cttcttctaa tttcatacca cgtgatcctt taactaatac
    22261 acggtcatgc gcttttaaat cgtttattaa aacttctatc atatcgtctt tagaattgaa
    22321 gtgttgtgct ttttcgacat gttgctggcc cgaatcataa atatacttcg cttcattacc
    22381 aaacgtatac aacacatcta tatgcttttc ttctaaataa ttacctacac cgatatgcat
    22441 ttctttgcta ttttcaccta attctaaaac atctcctaaa attagaatgc gacgccctgt
    22501 caaagtactc agtgtatcaa tagctgctct catacttgta ggacttgcat tataggcatc
    22561 atttatcaca gtaatatcat tttctaatgt atgttgttcc atacgcatac cagttaagct
    22621 gacatttttt aaattttgat agattgtgtt atatatcaaa cctaattcat gaccaaccgc
    22681 aatggcaatc gtcgcatttt tcatattatg ctttcctaat attggcagat cgtaatgttc
    22741 tttattatta atcgtaaatg aaatacctgt agtatctcta tcatcaacag aacaaactaa
    22801 tgcattatca gtagcaacac caatactaat acattttgca ttttcaactt ctttaacatg
    22861 tggtttcaat aatggttcat cgccatcata tataaacgta ccattatctt ttagacctat
    22921 tgtaatttca gatttagctt tagcaatccc ctcgcgcgaa cctaaatctt gcatatgtga
    22981 ctcaccaata ttagttataa ctgcaatatc tggttgagcg aggtttgaca gaaattcaat
    23041 ttcatggaaa cctgacatcc ccatctccaa tattgatatt tcagtatcat tatctaattc
    23101 caaaatagtt aaaggtaaac caatttcatt attgtaatta ccttgcgttt tcttaacttt
    23161 aaattcggta tgcaatacac tttcaatcat atctttagtc gttgttttac cattagaccc
    23221 tgtgacggca attactttag ggtttacatg tctcaagtaa gcttgtgcca attgttgtaa
    23281 tgccgttaat gtgtcttcaa cccatataat aggcccgctt acattttcat ctataggtgt
    23341 ccctctttga taaaaagcag ccccagcacc atcttgtaat gctttagaga caaagcgatg
    23401 accgtcaaca ttttcacctt taaatggtat aaataacata tttttagaaa ttgctcgtga
    23461 atcaattgtg actccattta tctcttgatt taaaaattga tcttcaattt cacaaggaat
    23521 ccatgattga atttgcttta atgtaacatt aatcataata acaacctcag ttagtcaatt
    23581 ttgtatttat ttttctgttt atcctggtga cgttctttag caagctcgat aagttttgta
    23641 atcaattctg gataagataa gcccatattt tcccataact ttggatacat actgaaagcc
    23701 gtaaatccag gcattgcatt tgtttcatta atatatattt ggttgtcttc tgttacaaag
    23761 aaatcagcac ggactaaacc agaacaatct gtcgctttga atgcctctaa tgccatattt
    23821 ctaagcgtta attgaacatc ttcgtctaag tcagctggaa tttgtaattg aaccttacca
    23881 tctttatatt ttgatttgta atcgtaaaac gcgacatctt ttacgacttc acctggccat
    23941 gtcgcttcag gatagtcatt tcctaaaact gctacttcaa tttcacgtgc gttaacgcct
    24001 tgttctataa caagcttacg gtcaaattgg aatgcttctt taataccttc tttaagttcc
    24061 gcttcattat tacatttact gatacctaca cttgacccta agttagcagg tttaacaaag
    24121 actgggtaat ttaatttatc atttactaat tttaaaatgt tatgttcata tttttcatat
    24181 tcagaacgta agaaactaat ataaggtaac tgtggtaacc ctcgatgttc aaataattgt
    24241 ttcattacaa gtttgtccat agaacttgca gctgacaata caccatttcc tacatatggt
    24301 acatccaaaa cttcaaaaag cccttgaatc gtgccatctt caccattagg accatgtaat
    24361 aatgggaata ctgcatcgta tggttgtcct gaactacttt ctttcaatag ctgtgaaatc
    24421 tcaagcgcct ctccattttc taaatgaagc tcatcagtag atttaatttc agctgtaata
    24481 ttattttgct ttctccaatc accatcattg gtaatataaa tgatatcaac atgatatttg
    24541 tctttatcta ttgcatttaa tacattttgt gctgtcagaa tcgatacttc gtgttctgca
    24601 cttttccctc caaaaacgat acaaatattt tcttttgtca tttcgttttc ctccaatgat
    24661 atatcagggt ttgctacctt aatataaatt aatattgatt tagtcctatt gttaattgat
    24721 ttattcgcat ctttgaatct taatagtatc acattttaat gctattttgc attttaaaaa
    24781 cgaggactta tgtgtctgta tctatgttga ttcatttata atcagaacaa tattcccttc
    24841 tattttattc ctaattcaaa ttgaaatcta tagctgatat cactgtgata ttctagctaa
    24901 tttttaaaaa gtcatgtaaa attgatataa acattagtga gtataaaagg agtttgcaat
    24961 gaattattca tctcgtcaac agccggataa gcattggctt cgcaaagtag actgggtatt
    25021 agtagccact atagctgttt tagcaatttt cagtgttctg cttattaact cggcaatggg
    25081 cggtggtcaa tacagtgcta atttcggtat cagacaaatt ttttattaca ttttaggtgc
    25141 aatttttgca ggtatcatca tgtttatttc acctaaaaag attaaacatt atacatattt
    25201 attgtatttc ttaatctgtc tattattaat aggcttgctc gttattcctg agtcacctat
    25261 tacacctatt atcaatggtg ccaaaagttg gtacacgttt ggccctatca gtattcagcc
    25321 atctgaattc atgaaaatta ttttaatttt agcattagcg cgtgtcgttt ctagacataa
    25381 tcaattcaca ttcaataaat cattccaaag tgatttgtta ttatttttca aaattattgg
    25441 tgtctcgtta gtaccaagta ttttaatatt actgcaaaat gacctaggaa ctacattagt
    25501 attagctgct attattgcag gtgtgatgtt agtaagtggt ataacatggc gtatcttagc
    25561 acctatcttt attacaggta ttgttggtgc aatgacagtc attttaggta ttctatatgc
    25621 acccgcatta attgaaaatt tattaggtgt ccaactgtat caaatgggac gaatcaattc
    25681 atggcttgac ccctatacat atagtagtgg tgatggctat catttaactg aatcacttaa
    25741 agctatcggt tctggacagt tactaggtaa aggatacaat cacggtgaag tttatatacc
    25801 tgaaaatcat actgacttta tcttttcagt gattggagag gaacttggct ttatcggttc
    25861 tgtcatattg atcttaatat ttttattttt aatcttccat ctaataagat tagctgcgaa
    25921 aattgaagat caatttaaca aaatctttat cgttggtttc gtcactttac ttgtgttcca
    25981 tattttacaa aatattggta tgacaattca gttgttacca atcactggta ttccattacc
    26041 atttattagt tatggtggta gtgcgctatg gagtatgatg actggaatag gtatagtctt
    26101 atcaatttat tatcatgaac caaaacgata tgtcgattta taccatccaa aaagtaatta
    26161 atttaaacta ttttgagttt caaatatcat aacttttcaa gatgacgtta tatagtctat
    26221 ttacgtcgtc gatttaaaat gtcatatata gatattactc gataataaca atccctcttt
    26281 gaagtacaca ttgtaaaatg tacactctaa agagggattt tgttatatag tacttgcttt
    26341 cattttagta aatccagtca aaaaagctat catcgaatag tccgacgata gctttaacgg
    26401 tgtgtgattc acaatcacag tcaattattt taattcgtga cttactttat tagatgctgg
    26461 caaagtatta ataatgtcta aacgagattt agttttttta atattcacac ctaatgatga
    26521 caatagtttg actacatttt gaatcttttc aggacgtttc attattatca cctcgtttgg
    26581 atcgctttca atattatctt accctattcg acataccttt actacatcat ttacaaaatt
    26641 tttaatttat tttcaaaatt aaaatctata gcgcactcta cattataccc taaaatacaa
    26701 ttttgtatcc ttcatcatag atttcttttt caatactatt caaatttgct ggtgtctcat
    26761 acgatacagt tacttgacca tctattatat taatattcac ttgatgaaca cctatcatat
    26821 ttgaaatgcg ttctgtcaat tgacttactt gttcttctgt ttcaattccc gctgtgtaaa
    26881 tcgtattttg atgtatcatc ttaaactcct ttagtcttta atcaattttt gaaaagtcac
    26941 taataactct tccattgcct cttcctgagc accttgatta actttattca taatacaact
    27001 tttcatatgt tgttctaata actttatcgc aacactgttt aacgccgaac gtgtcgctct
    27061 tatttgcgta aggacatcat cacaatagac atcttcttca atcatgcgat taatcgctct
    27121 cacttgtcct tcaattcgat ttaaacgtga tttaagattc gtttttattt gttcagaatg
    27181 atgtgcatta tcttgttcag tcatagcatt cactccttca tttacattcc ttactatact
    27241 atatcccata aaacacaacg tcaacacaaa gtgtgttttt caaactaaat tgtcatgata
    27301 tttaaattgc ttcatgatac aattaataag atatactact cgtttaagat tttcgatggg
    27361 aggtttcaaa tgatagagtt attatccatt gcactcaagc attctaatat tattttaaat
    27421 tcaatattta ttggtgcatt tattttaaac ttattattcg cctttaccat tattttcatg
    27481 gaaagacgtt ctgccaattc tatctgggct tggttactag tcttagtttt cttgccttta
    27541 ttcggcttca ttttatactt actattagga cgacaaattc aacgtgacca aattttcaaa
    27601 attgataagg aagataaaaa aggattagag ttaatcgttg atgagcaatt agctgcttta
    27661 aaaaatgaaa acttttcaaa ttccaattat caaattgtaa aatttaaaga aatgattcaa
    27721 atgttgttat ataataacgc agcattttta acaacagaca acgatttaaa aatatacaca
    27781 gacggccaag aaaaatttga tgacctaata caagacatcc gtaatgctac tgattatatt
    27841 cattttcagt actatattat tcaaaatgat gaattaggtc gtaccatttt aaatgaactt
    27901 ggtaaaaaag cggaacaagg tgtagaagtt aaaattcttt atgatgacat gggttctcgt
    27961 ggactgcgta aaaaaggctt acgcccgttt cgcaataaag gtggacatgc tgaagcattt
    28021 ttcccatcaa aattaccttt aattaacttg cgtatgaaca atcgaaacca tcgaaaaatt
    28081 gttgtaatag atgggcaaat tggatatgtt ggtggtttta atgttggtga tgagtactta
    28141 ggtaaatcaa aaaaattcgg ctattggcga gatacgcatt tacgaattgt cggggatgca
    28201 gtgaatgcat tgcaattacg atttattcta gattggaatt cacaagccac acgtgaccac
    28261 atctcctatg atgatcgtta tttcccagat gtaaattctg gtggaacaat tggcgttcaa
    28321 atagcttcta gtggtcctga cgaagaatgg gaacagatta aatacggcta tttgaaaatg
    28381 atttcatctg ctaaaaaatc gatttatatt caatctccct atttcatacc tgatcaagcc
    28441 tttttagatt ctattaaaat tgcggcatta ggtggtgttg atgtcaatat catgattcct
    28501 aataaacctg accatccgtt tgttttttgg gctactttaa aaaatgcagc atccttatta
    28561 gatgccggtg ttaaagtatt tcactacgac aatggctttt tacactcaaa aacacttgtt
    28621 atagatgatg aaattgcaag tgtgggaaca gctaatatgg accatcgcag tttcacattg
    28681 aatttcgaag tcaacgcttt tatttatgac caacaaattg ccaaaaaatt aaaacaagct
    28741 tttatagatg atttagcagt atcttctgaa ttaacaaaag cacgttatgc taagcgaagt
    28801 ctttggatta aatttaaaga aggtatttca caattattgt cacctatctt ataaaataga
    28861 aatatgagga gtgtaacttt aatgcaacaa tcagacgtca ttagtgctgc caaaaaatat
    28921 atggaatcta ttcatcaaaa tgattataca ggccatgata ttgcgcatgt atatcgtgtc
    28981 actgctttag ctaaatcaat cgctgaaaat gaaggtgtta atgatacttt agtcattgaa
    29041 ctcgcatgtt tgcttcatga taccgttgac gaaaaagttg tagatgctaa caaacaatat
    29101 gttgaattga agtcattttt atcttcttta tcactatcaa ccgaagatca agagcacatt
    29161 ttatttatta ttaataatat gagctatcgc aatggcaaaa atgatcatgt cactttatct
    29221 ttagaaggtc aaattgtcag ggatgcagat cgtcttgatg ctataggcgc tataggtgtt
    29281 gcacgaacat ttcaatttgc aggacacttt ggtgaaccta tgtggacaga acatatgtca
    29341 ctagataaga ttaatgatga tttagttgaa cagttgccac catctgcaat taaacatttc
    29401 tttgaaaaat tacttaagtt agaatcttta atgcatacag atacggcgaa gatgattgct
    29461 aaagaacgtc acgactttat gatgatgtac ttgaaacagt tttttacgga atggaattgt
    29521 cacgactaga cattgaagtt gtagtatgat gatgcgatgt aatggcgtgt tgttgtggaa
    29581 gcttggtgtc atgccatgtt actttgatgt gttgttgtgg gagcttggtg acatgtcatg
    29641 ctactttgat gtgctggtac cacgatgcgt cttgatgtag tgctatgatg tggcattgcg
    29701 gtgttatggt gttatagaca ggtttggcgt tgatgccatg ttactttgat gtgctggtac
    29761 cacgatgcga cttgatgtag tgctatgatg tggcattgcg gtgttatggt gttatagacc
    29821 ggtttgatgt tgatgccatg ttactttgat gtgctggtgc tacgatgcga cttgatgtag
    29881 tgctatgatg tggcgttgcg ctgttatggt gttatagcca ggtttggtgt tgatgtcatg
    29941 ccgttacgat tctatgatat gttgttggga cgttgcaatg tgtattatgc cgttgtgacg
    30001 ttattatttc acactgttac atgtataagt gaattgctgt ggaaatttgc gacatatact
    30061 gctacactga tgaatcattg tgtcaagatg acattgcgat gaagaatgac aactctgtta
    30121 ttaaccactt tttacatact gaaaactcgt taatattatt tcaaataaaa acagcagtag
    30181 gatgactttc acatttgaaa tcatcttact gctgtttcta tttatcacat attgtataat
    30241 gtgacactaa gtttcgctat tgaagcgaaa aataatgtgc gccctataaa gttaaaatta
    30301 tcttcaactt ttagggtgca cattatttgg acttgctaag gttatttctt tttcttttta
    30361 gacacaactt gtgtgttttt gcctttttta ttgctgccgc cgttgtgctc tctttcatac
    30421 gcttcaatga aaggttgtac ttctttttta gcgacttttt cataataaat gttcgcaaag
    30481 tgtgtttgaa ctacaaggaa cgccgcactg actgaccagt acaaaccaag tgctgatgct
    30541 gagcttaatg aaatccagat aatcataatt ggtgaaatga ccatcatcat gtaacccatt
    30601 tgacgttgtt cgtctggcat cgttttactt gatacatatg cttggataaa gtataaaaca
    30661 ccggcaataa ttgtaatcca aatatcagga cgtcctaaat cgaaccataa gaagtgtgga
    30721 tatttaaaca aaccatctac aagttggtct ttaagtacaa agtataatcc catgatgatt
    30781 ggtaattgga ttagcattgg taaacaaccc aacatactct taatcgggtt catgtcatac
    30841 tttttatata cttgcattaa ttcttggttt gcagccattt tttcttcttg tgtacgcgca
    30901 cgtttcactt tttcttgaat tttttcaact tctggctttg caactttcat tttttgacgc
    30961 atcatatgac tatttttata gtttgacaac atgaatggta ataaaataat acgaattacc
    31021 aatacaagga taataatagc taaaccataa ttgtcgttta ataagttatt tcccaaccaa
    31081 tccaatacat ttttcattgg atctacgaat gtattgtaga aaaacccact acgtttttca
    31141 ggtttagaat agtcacaacc agccaaaaag accataatac ctaaaaataa tggtagtaac
    31201 gcttttttct tcatttttcc acctctatca ttatattcac ataggattta ttctatcaca
    31261 ttaatgagta cgtatgaaac aataagtgga aaaatttaac taattattaa aaaaatcttt
    31321 gaatcgatta acagtctttt caatattttc acttttagaa atggctgaaa tgactgaaat
    31381 tccattggca cctgcttcta caatcggcgc cacattatta gtattgatac cgccaatagc
    31441 tacaatcggt agttgcggat tcatttcttt aaacgttgca atcatttctg gacctactgg
    31501 tatatgcgcg tcatgcttcg acggcgtagg atagattggt ccaacaccta tataatcaac
    31561 atgagttaaa tcagattttg catactcatc taaatcacta atactaagtc caataatttt
    31621 atcagtgaaa tattgtgcta tctctttgac tttcgcatca tcttgaccga catgtatacc
    31681 atccgcgtta atttcttttg ccaaggatac atcatcatta acgataaaag gcacatcata
    31741 ttgatgacag agatgctgta attctttagc taatacaagt ttatcgtttc cttttaaagc
    31801 tgattcaccc ttttctcgaa attggaaaag tgtaatccct gcctttaatg ctgcttctaa
    31861 tacttcatga atagttcgat gactcggaac atcggaggtc ccacaaataa aatacacatt
    31921 tagatacgat tggttaaaca tattattcca cctcttgaat acgaatccgt tgttgatagg
    31981 ttgtttcatt taaatgatat aacgtatcaa gcaacaatgg tgaaaacgta ccaggaccac
    32041 cacaattttc attttcagca gctacctcag cagcaatatt aaatacgctt accgcttcaa
    32101 ttaacgcttc tatgtctggt tctgtttctc taaataaaaa tccagcaata acgcctccta
    32161 ataaacaacc agctccagtt actcgtgcta ataatggaga tccattagct aatacgaagg
    32221 ctttattatc ttgaacaata acgtcctctt tacctgtgat tactattgca gttttatatg
    32281 ttgcgtaagc ctttttcgct attgcaaccg catcaagatt agcatcacta tctgtacctt
    32341 tcatagttgc tgtatcatca atcaacgcta atatttcaga tgcattgcct ttaattactg
    32401 atactttcgc tgatttcaat aataatttac aaaattgctt tcgatatgta gaagcaccaa
    32461 cagctacagg gtcaaataca ataggtaagc ctgcctcatt tgccgtttga gcaatcgcaa
    32521 taatatcttg ttcattttct gctgttaaag taccgatatt gattaatagc gcttgtgcaa
    32581 ctttgtaaaa ttcttcagct tcttcgggag cttcactcat tgcagggcta gcaccaatac
    32641 ttaataaacc attcgctgta aaatttttaa ctacatcgtt tgtataacaa attgttaatg
    32701 ggttttcaat acgtatttta tttagataat tcatcgtcta atccctcttt ctttaaatat
    32761 gcaaaatgat tcactggacc tctaccacgg ccgatttcag gcgtatattg tatactcatt
    32821 gaaataaact ttttagcctt gtgtacagcc tcaaataatg gtctaccttt tgcaagttct
    32881 gccgttataa ctgctgaaaa tgtacaccct gttccatgcg tatgttttgt tttaaatcgt
    32941 tcattttcaa atgtttgaac accttcgtta gtaaataaat aatcttttgc tatatcagta
    33001 tcatttgaat gaccgccttt aatgatgaca cctttactac caatctcatt aataaagatg
    33061 cggccagcct gcataatttt ttcttcacta tcaatggtta gtcccgttat ttcttcagct
    33121 tctggtaaat tcggtgttac tacgtcagct aatggcaata acgtatgctg caagttttgc
    33181 tttgtgtcat tatccattaa tgaatcaccg cttttcgcca acataacagg atcaattaca
    33241 tatggaatag attcatgttg cattaaataa tgacgaatcg tttccatagt atctgctgta
    33301 gcaatcatcc ccgttttaat agcatgaggt aaggtatcat tgaagacact atcaagttgt
    33361 tcatctaccc attgatgatt taaattatga atatgttgta cgcccaatgt attttgagca
    33421 acaatacttg taacgacgcc cataccatat acaccacatg aatgaaatga ttttaaatca
    33481 gccattacgc cggcaccacc tgttggatca gtacctgcaa tggttaatgc tattttaggt
    33541 ttaatcattt acttttcctc caaattccca ttgttctagt gtcatagcca tattgaaaaa
    33601 tcgacgctca tgtatgcaac tttctaaaaa tacttgcttt acttgttcca attccttgtc
    33661 agacattgat tcggctaatt tattcattaa tgcctcaaat acgttaatta tgtcatccat
    33721 ttcagtacta taaaaatcaa accattttgc cgtatctttt tctctattaa gcttatgatc
    33781 actttgagag cgcttagcta attctgcata aatatatgga caaggtgcca ttgcagcaat
    33841 tgtataaata gcattttcac gactatgcgc ttggaaatac atatgtttta tgtaatggtc
    33901 gccacttgga ggccaaactt ttgttttaat gatttcttcg tatgattcac caacaatttg
    33961 cgctaaaata tcatgcgcaa gtacttcacc ttcaaccata aattctattt gctctactaa
    34021 aaattttacg tcattcatgc tattcatttt tggaattaac aaagcatata agtttgtaaa
    34081 ttcttttaaa tacgcagcat cagcttttaa gtaatggcgt aatgcgtcag ctcctatatc
    34141 tccggataac atcttctgaa taaagtcatc ctcataaata tcattaatga ttggctttgc
    34201 agcttggtac aatttttgtg aaaattccat tgtaaaaaat cctccctaaa taaaaaaact
    34261 acttccaaca tgaaagtagt ttgatggcaa tgttgctata ctagccccat cacttcaata
    34321 actactttcc tacgttggta ctaaccaaat caggtcataa gggtctgaac aattcatctc
    34381 agccatatca ttaggctccc ctagtagttc cttagtattc aattgcaaat taatcttagc
    34441 aaacggtttc aacattttca attattgttg ctcagttgta ttattatctt taaataataa
    34501 ttctataatg acatatattt gcgaaataaa aaaaccggaa catatcgaga attccccgat
    34561 atattccaat ctaaaagtta cttatataac tattaattag ctatgcataa atggcttatg
    34621 cagtaaccca atgtccagcg ccaccagtgt tatataattt tactgctgcg gcatcttgaa
    34681 cactttcagg agcatttgct ggtgatacac ctttatattt agcaggtgct actgaatccc
    34741 aagttgattg taagaattga tacttaccag ctgcacctga tgttggattt acagcatgaa
    34801 tattgccacc tgattcacgt tgagcaattt gttttagatg agcattcaca tttactgatg
    34861 aaccttctga tgattttgat tcagttggtg ttgcagtaac ttgtgaattg tttgatgttg
    34921 atgcttgtgg ttgttgagtt tgagcatttt gtggtgcttc aacttcttgt gattgtactt
    34981 gattagcttg aacagctgat ggtgcaacat tattagttgc aggtgcttgt gcactcatgt
    35041 ctgctccatt agtacctgtt gcatggtaat tccaagcaaa gtgtgtacca tctgattcaa
    35101 agtgataagt aaacccttca tagtcaaatg tataattata agccccagct tcaattggtt
    35161 tttgatttaa tgtttgatca tttgattgcg ccatttgcgc taaagatgct ttatttaagt
    35221 ccgcttcact tgcatgggct tcgtgacctg catttcctgc tacgattcct aaacctactg
    35281 ctaatgatga tgcgagtaat gttttcttca taatcttaaa atcctcctac aagtaatttg
    35341 tttctctaaa agttttacag agactactgt aacatctata aaatccgtgc aggttacaac
    35401 aaaataaaaa aatagccttg ttttttacac agtgattaca acgaaatccc ccataatttc
    35461 agatatgatt ctatcgagaa ttgtcatgac agtaaaaatg tttttttatt tgcaattttt
    35521 tatgtaatcg ttttgcaatc tatgtctatt ttctattaga aaaactacac aaaaagacga
    35581 cctacacaac agtactagtc atgtaagtcg tttttaagtg agtatatttc aagttaatta
    35641 gatctataaa aaatttagga tggattaaat gtatattaag gatgtttagg ataattggat
    35701 ttaaatttct aataagtcat gattatctat attttgagag tcaatatcta aatactatct
    35761 gagagaattt cattattttg ggatttaggg gacataaatt ttattgtttc aacatataat
    35821 tcagtgacaa agtgtgtttg tccgtcttta tcatactttc ttgatctcat ttgaccagtt
    35881 ataccaacca atgtaccttg attagtatat ttttctatat tagaagctaa cttgccaaat
    35941 gctttacaga ataagtaatc acagacgatt tctccatttt catctttata atttcggtgc
    36001 gttgcaacac aaaacgttgc aatttttcta tcctcctttt caaatatttg tgcgtctttc
    36061 gtcagtctcc cgacaattac gattttattt agcatgtagt gtgtgattca cctcctatgc
    36121 ctatatctta attactagct taaaaatggt caaaatggca aattttatat aattaatagc
    36181 tatagcatct cgttatttgt tatatttaaa gaaattagtc atgaaatgac cttaaatctt
    36241 ctaaataatt caaaaatcat aaatattaaa ctaatgggat gtgggaatat gaaaactttt
    36301 aaagcagtaa ggtttcaaat tgtgaatgaa cacggcagaa ttatagaata tgaattagaa
    36361 gatggcgtaa taattaataa agaggaaagt ggcacgggtt ggttgcttga aattgtcatt
    36421 tctaatgagc attatgaaac atttaaggaa tatcaagaca atgaacagtt attggatatt
    36481 cgtgtcgtca tcacgcgtcc agccaatgat ccagctttat ttgaatctac tgtgaaaagt
    36541 attaaaaatt ttaaaacaac aatgtctatc gtatttgaat gtcatattta cactttacgt
    36601 caacagtatg ctgaaagttt attagaacaa cttattgatg atggattatc tggtgaagaa
    36661 ttaaaaaaat catttaatcg aatgatgcag tctaaaccaa agttaaagga tgaaaaatta
    36721 gaagagtaaa ataaaaaaca ttcaaagatt atgccaacac attaaatctt tgaatgtttt
    36781 tttgttttat tttacatctt gaattgcaaa tgtaagttca caactacaag caagttgacc
    36841 atcgacagta gctttagcat tacctttacc tattggtcct ttaattttag tgatttctac
    36901 ttccaacgtt aaagtatcac caggtactac ttgacgttta aaacgacatt tatcaatacc
    36961 agcaaataaa gcgattttac ctttattttc ttcactattt aaaatagcta ccgcacctgt
    37021 ttgagctaac gcttcagtaa ttaatacgcc tggcattacc gcatactcag gaaaatgccc
    37081 ttgaaagaat ggttcgtttc ctgatacttg tttaatagcc acacaacgtt gaccttcttc
    37141 atattcaact actttatcaa ttaataaaaa tggctgtctg tgaggtataa tttgtttaat
    37201 ttggttataa tcaaaaattg tttccatggt tgaaatcctc cattatatta atttactgaa
    37261 ttaatcgtta atacgttcaa tgtctgcacc taattgcttc aatttaccgt gtaagtcaac
    37321 atagcctcta tctaggtgcg ttaattcagt aacgcttgtt ttaccatcag caactaatcc
    37381 agctaaaatt aaggcggctg ctgctcttaa atcagtcgct ttaacttgtg caccttgcaa
    37441 ttgactttta ccttcaagtt tagcactacg accttctaca ttgatattag cattcatacg
    37501 tttgaactct gcaacatgca taaaacggtt ttcaaaaaca gtttcggtta cgactttatg
    37561 accatttgcc gttaataaca atgccatcat ttgtgattgc atatcagtcg ggaatccagg
    37621 atgtggtaga gttttgatgt ctacaggttg taattcccct tcagcacgta cacgaatacc
    37681 atcttcttga tagtccaatt caacgcccat ttcttctagt ttatagacta aactcgccat
    37741 atgttctttg attgcatcac gtacaaaaat atcaccacgc gttatagcac cagcgattag
    37801 taatgtgcct gcttcaattc tatctggaat gatagcatgt tctacaccat gtaatgattc
    37861 tacaccattg attgtaattg tgtctgtacc agcaccagta attctaccac ccatttcatt
    37921 aatgtagttt gctaaatcga caatttcagg ttctttagct gcattttcaa ttaaagtctt
    37981 acccttagct aatgatgctg ccataataat attttgtgtt gctcctacac ttggaaaatc
    38041 taaatgaatt gatgtacctt ttaatccatc tttagcatta gcataaatat taccattttc
    38101 aagatgaatt tctgcgccta aagcttcaaa acctttaatg tgttgctcaa tcggtctact
    38161 tccaattgca caaccaccag gcaatgcaac aatagcatgt cctagtcttg ctaaaagagg
    38221 tcccataact aaaatacttg cacgcatttt actaacatat tcatatggtg cctcttcatt
    38281 tagagtcttt gttgcatcaa cgacaacagc attttcgtcc tttttgtatg taacgtcagc
    38341 atttaaagtt gttaatacat tatttattgt ttctacatca cttaaagctg gaacattaac
    38401 taatttgctc ggtttatcag aagctaataa agatgctgtc aatattggta atactgcatt
    38461 tttagcacct tctactttaa cttcacccgt taatttattt ccacctttga ttactatttt
    38521 atccatcgta aatcctccac ttagaactcc tattacttta catttcccta ttttaaaatt
    38581 attttaagtc attatttaat tatatctagt tttgattaca aatcatagag ttagacttat
    38641 tttattaaat attttacctg cgtcgagtat tgtaacaaat ctacaataaa attgcttact
    38701 gcagtgccta ataaaatagc aacaaatatc atacacactt gtaattgagt ggcgtatcct
    38761 tttttaaaaa attgatctaa tctaattgat tgtaaagccc aataggcaat acaaatacat
    38821 acaacatgta acactaaatg gataactgca tattgtccga tataatccat tttatcacct
    38881 cataagatta ctgtattaca ttgtacatga aaaatttaaa taatgataca aagtttaata
    38941 cccgttttta gtaaagttta agctaactta ctatataatt ctttttacat aaatatcata
    39001 actataaaat atcttggtat gaaaaaatgt agataatttc aaaaaatgaa atgtcgactg
    39061 tagatagtgg tgaaggaaat tttgatttta gcaatctatt agtgattgta aaaaagtaaa
    39121 actattcttg ggagtcggac ataaatcaat gtctagactc tatagtgttg tatttggcag
    39181 tagttgacgg ggccc
//
LOCUS       DALAGD010000008        37981 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.008, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000008 DALAGD010000000
VERSION     DALAGD010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 37981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37981
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.008"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            442..1779
                     /locus_tag="O2701_000318"
     CDS             442..1779
                     /locus_tag="O2701_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HCZ4292232.1"
                     /translation="MKQPILNKLESLNQEEAISLHVPGHKNMTIGHLSQLSMTMDKTE
                     IPGLDDLHHPEEVILESMKQVEKHSDYDAYFLVNGTTSGILSVIQSFSQKKGDILMAR
                     NVHKSVLHALDISQQEGHFIETHQSPLTNHYNKVNLSRLNNDGHKLAVLTYPNYYGET
                     FNVEEVIKFLHQLNIPVLIDEAHGAHFGLQGFPDSTLNYQADYVVQSFHKTLPALTMG
                     SVLYIHKNAPYRETIIEYLSYFQTSSPSYLIMASLESAAEFYKTYDSTVFFDKRAQLI
                     ECLEKKGFEMLQVDDPLKLLIKYEGFTGHDIQNWFMNAHIYLELADDYQALAILPLWH
                     HDDTYLFDSLLRKIEDMILPKKSVSKVKQTQLLTTEGNYKPKRFEYVTWCDLKKAKGK
                     VLARHIVPYPPGIPIIFKGETITENMIELVNEYLETGMIVEGIKNNKILVEDE"
     gene            1781..2398
                     /locus_tag="O2701_000319"
     CDS             1781..2398
                     /locus_tag="O2701_000319"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCZ4292233.1"
                     /translation="MSAFITFEGPEGSGKTTVINEVYHRLVKDYDVIMTREPGGVPTG
                     EEIRKIVLEGNDMDIRTEAMLFAASRREHLVLKVIPALKEGKVVLCDRYIDSSLAYQG
                     YARGIGVEEVRALNEFAINGLYPDLTIYLNVSAEVGRERIIKNSRDQNRLDQEDLKFH
                     EKVIEGYQEIIHNESQRFKSVNADQPLENVVEDTYQTIIKYLEKI"
     gene            2426..2755
                     /locus_tag="O2701_000320"
     CDS             2426..2755
                     /locus_tag="O2701_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HCZ4292234.1"
                     /translation="MKMIIAIVQDQDSQELADQLVKNNFRATKLATTGGFLRAGNTTF
                     LCGVNDDRVDEILSVINQTCGNREQLVSPITPMGGSADSYIPYPVEVEVGGATVFVMP
                     VDAFHQF"
     gene            2969..3895
                     /locus_tag="O2701_000321"
     CDS             2969..3895
                     /locus_tag="O2701_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCZ4292235.1"
                     /translation="MDEQQQLTNAYHSNKLSHAYLFEGDDAQTMKQVAINFAKLILCQ
                     TDSQCETKVSTYNHPDFMYISTTENAIKKEQVEQLVRHMNQLPIESTNKVYIIEDFEK
                     LTVQGENSILKFLEEPPDNTIAILLSTKPEQILDTIHSRCQHVYFKPIDKEKFINRLV
                     EQNMSKPVAEMISTYTTQIDNAMALNEEFDLLALRKSVIRWCELLLTNKPMALIGIID
                     LLKQAKNKKLQSLTIAAVNGFFEDIIHTKVNVEDKQIYSDLKNDIDQYAQKLSFNQLI
                     LMFDQLTEAHKKLNQNVNPTLVFEQIVIKGVS"
     gene            3896..4699
                     /locus_tag="O2701_000322"
     CDS             3896..4699
                     /locus_tag="O2701_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HCZ4292236.1"
                     /translation="MPNVIGVQFQKAGKLEYYTPNDIQVDIEDWVVVESKRGIEIGIV
                     KNPLMDIAEEDVVLPLKNIIRIADDKDIDKFNCNERDAENALILCKDIVREQGLDMRL
                     VNCEYTLDKSKVIFNFTADDRIDFRKLVKILAQHLKTRIELRQIGVRDEAKLLGGIGP
                     CGRSLCCSTFLGDFEPVSIKMAKDQNLSLNPTKISGACGRLMCCLKYENDYYEEVRAQ
                     LPDIGEAIETPDGNGKVVALNILDISMQVKLEGHEQPLEYKLEEIETMH"
     gene            4716..5063
                     /gene="yabA"
                     /locus_tag="O2701_000323"
     CDS             4716..5063
                     /gene="yabA"
                     /locus_tag="O2701_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HCZ4292237.1"
                     /translation="MDRNEIFEKIMRLEMNVNQLSKETSELKALAVELVEENVALQLE
                     NDNLKKVLGNDEPTTIDTANSKPAKAVKKPLPSKDNLAILYGEGFHICKGELFGKHRH
                     GEDCLFCLEVLSD"
     gene            5337..6062
                     /locus_tag="O2701_000324"
     CDS             5337..6062
                     /locus_tag="O2701_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HCZ4292238.1"
                     /translation="MLKENERFDQLIKEDFSIIQNDDVFSFSTDALLLGHFTKPRTKD
                     IVLDLCSGNGVIPLLLFAKHPRHIEGVEIQKTLVDMARRTFQFNDVDEYLTMHHMDLK
                     NVTKVFKPSQYTLVTCNPPYFKENQQHQHQKEAHKIARHEIMCTLEDCMIAARHLLKE
                     GGRLNMVHRAERLMDVLFEMRKVNIEPKKVVFIYSKVGKSAQTIVVEGRKGGNQGLEI
                     MPPFYIYNEDGNYSEEMKEVYYG"
     gene            6055..6303
                     /locus_tag="O2701_000325"
     CDS             6055..6303
                     /locus_tag="O2701_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCZ4292239.1"
                     /translation="MDSHFVYIVKCSDGSLYTGYAKDVNARVEKHNRGQGAKYTKVRR
                     PVHLVYQEMYETKSEALKREYEIKTYTRQKKLRLIKER"
     gene            6305..7144
                     /gene="rsmI"
                     /locus_tag="O2701_000326"
     CDS             6305..7144
                     /gene="rsmI"
                     /locus_tag="O2701_000326"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCZ4292240.1"
                     /translation="MAVLYLVGTPIGNLADITYRAVDVLKRVDMIACEDTRVTSKLCN
                     HYDIPTPLKSYHEHNKDKQTAFIIEQLELGLDVALVSDAGLPLISDPGYELVVAAREA
                     NIKVETVPGPNAGLTALMASGLPSYVYTFLGFLPRKEKEKSAVLEQRMHENSTLIIYE
                     SPHRVTDTLKTIAKIDATRQVSLGRELTKKFEQIVTDDVTQLQALIQQGDVPLKGEFV
                     ILIEGAKANNEISWFDDLSINEHVDHYIQTSQMKPKQAIKKVAEERQLKTNEVYNIYH
                     QIS"
     gene            7429..9402
                     /gene="metG"
                     /locus_tag="O2701_000327"
     CDS             7429..9402
                     /gene="metG"
                     /locus_tag="O2701_000327"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCZ4292241.1"
                     /translation="MAKETFYITTPIYYPSGNLHIGHAYSTVAGDVIARYKRMQGYDV
                     RYLTGTDEHGQKIQEKAQKSGKTEIEYLDEMIAGIKQLWAKLEISNDDFIRTTEERHK
                     HVVEQVFERLLKQGDIYLGEYEGWYSVPDETYYTESQLVDPQYENGKIIGGKSPDSGH
                     EVELVKEESYFFNISKYTDRLLEFYDQNPDFIQPPSRKNEMINNFIKPGLADLAVSRT
                     SFNWGVHVPSNPKHVVYVWIDALVNYISALGYLSDDESLFNKYWPADIHLMAKEIVRF
                     HSIIWPILLMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVDPNILIDRYGLDATRYY
                     LMRELPFGSDGVFTPEAFVERTNFDLANDLGNLVNRTISMVNKYFDGELPAYQGPLHE
                     LDEEMEAMALETVKSYTESMESLQFSVALSTVWKFISRTNKYIDETTPWVLAKDDSQK
                     DMLGNVMAHLVENIRYAAVLLRPFLTHAPKEIFEQLNINNPQFMEFSSLEQYGVLNES
                     IMVTGQPKPIFPRLDSEAEIAYIKESMQPPATKEEKEEIPSKPQIDIKDFDKVEIKAA
                     TIIDAEHVKKSDKLLKIQVDLDSEQRQIVSGIAKFYTPDDIIGKKVAVVTNLKPAKLM
                     GQKSEGMILSAEKDGVLTLVSLPSAIPNGAVIK"
     gene            9433..10206
                     /locus_tag="O2701_000328"
     CDS             9433..10206
                     /locus_tag="O2701_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCZ4292242.1"
                     /translation="MLIDTHVHLNDEQYDDDLSEVITRAREAGVDRMFVVGFNKSTIE
                     RAMKLIDEYDFLYGIIGWHPVDAIDFTEEHLEWIESLAQHPKVIGIGEMGLDYHWDKS
                     PADVQKEVFRKQIALAKRLKLPIIIHNREATQDCIDILLEEHAEEVGGIMHSFSGSPE
                     IADIVTNKLNFYISLGGPVTFKNAKQPKEVAKHVSMERLLVETDAPYLSPHPYRGKRN
                     EPARVTLVAEQIAELKGLSYEEVCEQTTKNAEKLFNLNS"
     gene            10373..10909
                     /gene="rnmV"
                     /locus_tag="O2701_000329"
     CDS             10373..10909
                     /gene="rnmV"
                     /locus_tag="O2701_000329"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HCZ4292243.1"
                     /translation="MKINEFIVVEGRDDTERVKRAVECDTIETNGSAINEQTLEVIRN
                     AQQSRGVIVLTDPDFPGDKIRSTITEHVKGVKHAYIDREKAKNKKGKIGVEHAELIDI
                     KEALMHVSSPFDEAYESIDKSVLIELGLIVGKDARRRREILSRKLRIGHSNGKQLLKK
                     LNAFGYTEADVRQALEDE"
     gene            10920..11813
                     /gene="rsmA"
                     /locus_tag="O2701_000330"
     CDS             10920..11813
                     /gene="rsmA"
                     /locus_tag="O2701_000330"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCZ4292244.1"
                     /translation="MLDNKDIATPSRTRALLDKYGFNFKKSLGQNFLIDVNIINNIID
                     ASDIDAQTGVIEIGPGMGSLTEQLARHAKRVLAFEIDQRLIPVLNDTLSPYDNVTVIN
                     EDILKANIKEAVENHLQDCEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEV
                     GERLNAEVGSKAYGSLSIVVQYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLV
                     TVDNEEAFFKLAKAAFAQRRKTINNNYQNYFKDGKQHKEVILQWLEQAGIDPRRRGET
                     LSIQDFAKLYEEKKKFPQLEN"
     gene            11913..12176
                     /locus_tag="O2701_000331"
     CDS             11913..12176
                     /locus_tag="O2701_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HCZ4292245.1"
                     /translation="MPKSILDIKNSIDCHVGNRIVLKANGGRKKTIKRSGILKETYPS
                     VFIVELDQDKHNFERVSYTYTDVLTENVQVSFEEDNHHESIAH"
     gene            12486..13334
                     /locus_tag="O2701_000332"
     CDS             12486..13334
                     /locus_tag="O2701_000332"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCZ4292246.1"
                     /translation="MIYETAPAKINFTLDTLFKRNDGYHEIEMIMTTVDLNDRLTFHK
                     RKDRKIVVEIEHNYVPSNHKNLAYRAAQLFIEQYQLKQGVTISIDKEIPVSAGLAGGS
                     ADAAATLRGLNRLFDIGASLEELALLGSKIGTDIPFCIYNKTALCTGRGEKIEFLNKP
                     PSAWVILAKPNLGISSPDIFKLINLDKRYDVHTKMCYEALENRDYQQLCQSLSNRLEP
                     ISVSKHPQIDKLKNNMLKSGADGALMSGSGPTVYGLARKESQAKNIYNAVNGCCNEVY
                     LVRLLG"
     gene            13348..14172
                     /gene="purR"
                     /locus_tag="O2701_000333"
     CDS             13348..14172
                     /gene="purR"
                     /locus_tag="O2701_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HCZ4292247.1"
                     /translation="MRYKRSERIVFMTQYLMNHPNKLIPLTFFVKKFKQAKSSISEDV
                     QIIKNTFQKEKLGTVITTAGASGGVTYKPMMSKEEATEVVNEVITLLEEKERLLPGGY
                     LFLSDLVGNPSLLNKVGKLIASIYMEEKLDAVVTIATKGISLANAVANILNLPVVVIR
                     KDNKVTEGSTVSINYVSGSSRKIETMVLSKRTLAENSNVLVVDDFMRAGGSINGVMNL
                     MNEFKAHVKGVSVLVESKEVKQRLIEDYTSLVKLSDVDEYNQEFNVEPGNSLSKFS"
     gene            14189..14569
                     /locus_tag="O2701_000334"
     CDS             14189..14569
                     /locus_tag="O2701_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCZ4292248.1"
                     /translation="MKIINTTRLPEALGPYSHATVVNGMVYTSGQIPLNIDGHIVSAD
                     VQAQTKQVLENLKVVLEEAGSDLNSVAKATIFIKDMNDFQKINEVYGQYFNEHKPARS
                     CVEVARLPKDVKVEIELVSKIKEL"
     gene            14641..14943
                     /gene="spoVG"
                     /locus_tag="O2701_000335"
     CDS             14641..14943
                     /gene="spoVG"
                     /locus_tag="O2701_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HCZ4292249.1"
                     /translation="MKVTDVRLRKIQTDGRMKALVSITLDEAFVIHDLRVIEGNSGLF
                     VAMPSKRTPDGEFRDIAHPINSDMRQEIQDAVMKVYDETDEVVPDKNATSEDSEEA"
     gene            15133..15264
                     /locus_tag="O2701_000336"
     CDS             15133..15264
                     /locus_tag="O2701_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292250.1"
                     /translation="MRLNDARIFEVRKKLFLKLQRIKNNAFYMLKEYCRLNYNNDEV"
     gene            15287..16639
                     /gene="glmU"
                     /locus_tag="O2701_000337"
     CDS             15287..16639
                     /gene="glmU"
                     /locus_tag="O2701_000337"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCZ4292251.1"
                     /translation="MRRHAIILAAGKGTRMKSKKYKVLHEVAGKPMVEHVLESVKGSG
                     VDQVVTIVGHGAESVKGHLGERSLYSFQEEQLGTAHAVQMAKSHLEDKEGTTIVVCGD
                     TPLITKETLETLIAHHEDANAQATVLSASIQQPYGYGRIVRNASGRLERIVEEKDATQ
                     AEKDINEISSGIFAFNNKTLFEKLTQVKNDNAQGEYYLPDVLSLILNDGGIVEVYRTN
                     DVEEIMGVNDRVMLSQAEKAMQRRTNHYHMLNGVTIIDPDSTYIGPDVTIGSDTVIEP
                     GVRINGRTEIGEDVVIGQYSEINNSTIENGACIQQSVVNDASVGANTKVGPFAQLRPG
                     AQLGADVKVGNFVEIKKADLKDGAKVSHLSYIGDAVIGERTNIGCGTITVNYDGENKF
                     KTIVGKDSFVGCNVNLVAPVTIGDDVLVAAGSTITDDVPNDSLAVARARQTTKEGYRK
                     "
     gene            16786..17751
                     /locus_tag="O2701_000338"
     CDS             16786..17751
                     /locus_tag="O2701_000338"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCZ4292252.1"
                     /translation="MLNNEYKNSSLKIFSLKGNEALAQEVADQVGIELGKCSVKRFSD
                     GEIQINIEESIRGCDVFIIQPTSYPVNLHLMELLIMIDACKRASAATINIVVPYYGYA
                     RQDRKARSREPITAKLVANLIETAGATRMIALDLHAPQIQGFFDIPIDHLMGVPILAK
                     HFKDDPNINPEECVVVSPDHGGVTRARKLADILKTPIAIIDKRRPRPNVAEVMNIVGE
                     IEGRTAIIIDDIIDTAGTITLAAQALKDKGAKEVYACCTHPVLSGPAKERIENSAIKE
                     LIVTNSIHLDEDRKPSNTKELSVAGLIAQAIIRVYERESVSVLFD"
     gene            17901..18554
                     /locus_tag="O2701_000339"
     CDS             17901..18554
                     /locus_tag="O2701_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HCZ4292253.1"
                     /translation="MASLKSIIRQGKQTRSDLKQLRKSGKVPAVVYGYGTKNVSVKVD
                     EVEFIKVIREVGRNGVIELGVGSKTIKVMVADYQFDPLKNQITHIDFLAINMSEERTV
                     EVPVQLVGEAVGAKEGGVVEQPLFNLEVTATPDNIPEAIEVDITELNINDSLTVADVK
                     VTGDFKIENDSAESVVTVVAPTEEPTEEEIEAMEGEQQTEEPEVVGESKEDEEKTEE"
     gene            18865..19437
                     /locus_tag="O2701_000340"
     CDS             18865..19437
                     /locus_tag="O2701_000340"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCZ4292254.1"
                     /translation="MKCIVGLGNIGKRFELTRHNIGFEVVDYILEKNNFSLDKQKFKG
                     AYTIERMNGDKVLFIEPMTMMNLSGEAVAPIMDYYNVNPEDLIVLYDDLDLEQGQVRL
                     RQKGSAGGHNGMKSIIKMLGTDQFKRIRIGVGRPTNGMTVPDYVLQRFSNDEMVTMEK
                     VIEHAARAIEKFVETSRFDHVMNEFNGEVK"
     gene            19437..22943
                     /gene="mfd"
                     /locus_tag="O2701_000341"
     CDS             19437..22943
                     /gene="mfd"
                     /locus_tag="O2701_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCZ4292255.1"
                     /translation="MTILTTLIKEDNHFQDLNQVFGQANTLVTGLSPSAKVTMIAEKY
                     AQSNQQLLLITNNLYQADKLETDLLQFIDAEELYKYPVQDIMTEEFSTQSPQLMSERI
                     RTLTALAQGKKGLFIVPLNGLKKWLTPVEMWQNHQMTLRVGEDIDVDQFLNKLVNMGY
                     KRESVVSHIGEFSLRGGIIDIFPLIGEPIRIELFDTEIDSIRDFDVETQRSKDNVEEV
                     DITTASDYIITEEVISHLKEELKTAYENTRPKIDKSVRNDLKETYESFKLFESTYFDH
                     QILRRLVAFMYETPSTIIEYFQKDAIIAVDEFNRIKETEESLTVESDSFISNIIESGN
                     GFIGQSFIKYDDFETLIEGYPVTYFSLFATTMPIKLNHIIKFSCKPVQQFYGQYDIMR
                     SEFQRYVNQNYHIVVLVETETKVERMQAMLSEMHIPSITKLHRSMSSGQAVIIEGSLS
                     EGFELPDMGLVVITERELFKSKQKKQRKRTKAISNAEKIKSYQDLNVGDYIVHVHHGV
                     GRYLGVETLEVGQTHRDYIKLQYKGTDQLFVPVDQMDQVQKYVASEDKTPKLNKLGGS
                     EWKKTKAKVQQSVEDIAEELIDLYKEREMAEGYQYGEDTAEQTTFELDFPYELTPDQA
                     KSIDEIKDDMQKSRPMDRLLCGDVGYGKTEVAVRAAFKAVMEGKQVAFLVPTTILAQQ
                     HYETLIERMQDFPVEIQLMSRFRTPKEIKQTKEGLKTGFVDIVVGTHKLLSKDIQYKD
                     LGLLIVDEEQRFGVRHKERIKTLKHNVDVLTLTATPIPRTLHMSMLGVRDLSVIETPP
                     ENRFPVQTYVLEQNMSFIKEALERELSRDGQVFYLYNKVQSIYEKREQLQMLMPDANI
                     AVAHGQMTERDLEETMLSFINNEYDILVTTTIIETGVDVPNANTLIIEDADRFGLSQL
                     YQLRGRVGRSSRIGYAYFLHPANKVLTETAEDRLQAIKEFTELGSGFKIAMRDLNIRG
                     AGNLLGKQQHGFIDTVGFDLYSQMLEEAVNEKRGIKEPESEVPEVEVDLNLDAYLPTE
                     YIANEQAKIEIYKKLRKTETFDQIIDIKDELIDRFNDYPVEVARLLDIVEIKVHALHS
                     GITLIKDKGKIIDIHLSVKATENIDGEVLFKATQPLGRTMKVGVQNNAMTITLTKQNQ
                     WLDSLKFLVKCIEESMRISDEA"
     gene            22933..24459
                     /locus_tag="O2701_000342"
     CDS             22933..24459
                     /locus_tag="O2701_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCZ4292256.1"
                     /translation="MKHKEAFNGVVVLTAALIVIKILSAVYRIPYQNILGDTGLYAYQ
                     QVYPIVALGMILSMNAIPSAITQNIGKYHSDEAYAKAVAYIQLVGILLFIAIFVFANN
                     IAHMMGDGHLTPMIQAASLSFIFIGMLGVLRGYYQSANNMTVPAISQVIEQVIRVGII
                     IVTIVIFVDRGWTIYEAGTIAILASTIGFLGSSIYLVAHRPFKFKMVNNTAKIVWKQF
                     ALSVLIFAISQLIVILWQVIDSVTIIKSLQAIRVPFDVAITEKGVYDRGASFIQMGLI
                     VTTTFSFALIPLLSDAIKMNNQVLMNRYANASLKITILISTAAGIGLINLLPLMNGVF
                     FKTNDLTLTLSVYMITVICVSLIMMDMALLQAQHAVRPIFVGMTAGLVIKFILNIILI
                     RLSGIIGASISTVVSLIIFGTIIHIAVTRKYHLYAMRRFFINVVLGMVFMSIVVQCVL
                     NIVTTHGRITGLIELLCAAVLGIIALFFYIFRFNVLTYKELTYLPFGSKLYQIKKGRR
                     "
     gene            24459..25652
                     /locus_tag="O2701_000343"
     CDS             24459..25652
                     /locus_tag="O2701_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCZ4292257.1"
                     /translation="MAHTITIVGLGNYGIDDLPLGIYKFLKTQDKVYARTLDHPVIES
                     LQDELTFQSFDHVYEAHNQFEDVYIDIVAQLVEAANEKDIVYAVPGHPRVAETTTVKL
                     LALAKDNTDIDVKVLGGKSFIDDVFEAVNVDPNDGFTLLDATSLQEVTLNVRTHTLIT
                     QVYSAMVAANLKITLMERYPDDYPVQIVTGARSDGADNVVTCPLYELDHDENAFNNLT
                     SVFVPKIITSTYLYHDFDFATEVIDTLVDEDKGCPWDKVQTHETLKRYLLEETFELFE
                     AIDNEDDWHMIEELGDILLQVLLHTSIGKKEGYIDIKEVITSLNAKMIRRHPHIFGDA
                     NAETIDDLKEIWSKAKDAEGKQPRVIFEKVFAEHFLNLYEKTKDKSFDEAALKQWLEK
                     GESNT"
     gene            25649..25912
                     /locus_tag="O2701_000344"
     CDS             25649..25912
                     /locus_tag="O2701_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HCZ4292258.1"
                     /translation="MRLDKYLKVSRLIKRRTLAKEVSDQGRITINGNVAKAGSDVKVE
                     DVLTIRFGQKLVTVKVTALNEHASKDNAKGMYEIIEERRLEEA"
     gene            25930..26322
                     /locus_tag="O2701_000345"
     CDS             25930..26322
                     /locus_tag="O2701_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HCZ4292259.1"
                     /translation="MKNKVEHIENQYTSQENKKKQRQKMKMRVVRRRITVFAGVLLAI
                     IVVLSILLVVQKHRNDIDAQERKAKEAQFQKQQNEEIALKEKLNNLNDKDYIEKIARD
                     DYYLSNKGEVIFRLPEDKDSSSSKSSKK"
     gene            26427..26828
                     /locus_tag="O2701_000346"
     CDS             26427..26828
                     /locus_tag="O2701_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HCZ4292260.1"
                     /translation="MSIEVGNKLKGKVTGIKKFGAFVELPEGKSGLVHISEVADNYVE
                     NVEEHLSVGDEVDVKVLSIADDGKISLSIKKAKDRPRRQHTSKPSHQKPVQKAEDFEK
                     KLSNFLKDSEDKLTSIKRQTESRRGGKGSRR"
     gene            27008..28303
                     /gene="tilS"
                     /locus_tag="O2701_000347"
     CDS             27008..28303
                     /gene="tilS"
                     /locus_tag="O2701_000347"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCZ4292261.1"
                     /translation="MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTC
                     LHVNHGVRSASIEEARFLEAYCERHHIDLHIKKLDLSHSLDRNNSIQNEARIKRYEWF
                     DEMMNVLEADVLLTAHHLDDQLETIMYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLA
                     VSKKEIKQFQERYHIPYFEDESNKDNKYVRNDIRNRIIPAIDENNQLKVSHLLKLKQW
                     HDEQYDILQYSAKQFIQEFVKFDEQSKYLEVSRQAFNNLPNSLKMVVLDCLLSKYYEL
                     FNISAKTYEEWFKQFSSKKAQFSINLTDKWIIQIAYGKLIIMAKNNGDTYFRVQTIKK
                     PGNYIFNKYRLEIHSNLPKCLFPLTVRTRQSGDTFKLNGRDGYKKVNRLFIDCKVPQW
                     VRDQMPIVLDKQQRIIAVGDLYQQQTIKKWIIISKNGDE"
     gene            28308..28847
                     /gene="hpt"
                     /locus_tag="O2701_000348"
     CDS             28308..28847
                     /gene="hpt"
                     /locus_tag="O2701_000348"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCZ4292262.1"
                     /translation="MHNDLKEVLLTEEDIQNICKELGAQLTKDYQGKPLVCVGILKGS
                     AMFMSDLIKRIDTHLSIDFMDVSSYHGGTESTGEVQIIKDLGSSIENKDVLIIEDILE
                     TGTTLKSITELLQSRKVNSLEIVTLLDKPNRRKADIEAKYVGKKIPDEFVVGYGLDYR
                     ELYRNLPYIGTLKPEVYSN"
     gene            29104..31197
                     /gene="ftsH"
                     /locus_tag="O2701_000349"
     CDS             29104..31197
                     /gene="ftsH"
                     /locus_tag="O2701_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCZ4292263.1"
                     /translation="MQKAFRNVLVIVIIGVIIFGLFSYLNGNGNMPKQLTYNQFTEKL
                     EKGDLKTLEIQPQQNVYMVSGKTKNDEDYSSTILYNNEKELQKITDAAKKQNGVKLTI
                     KEEEKQSVFVSILSTLIPVVVIALLFIFFLSQAQGGGSGGRMMNFGKSKAKMYDNNKR
                     RVRFSDVAGADEEKQELIEIVDFLKDNKKFKEMGSRIPKGVLLVGPPGTGKTLLARAV
                     AGEAGAPFFSISGSDFVEMFVGVGASRVRDLFDNAKKNAPCIIFIDEIDAVGRQRGAG
                     VGGGHDEREQTLNQLLVEMDGFGENEGIIMIAATNRPDILDPALLRPGRFDRQIQVGR
                     PDVKGREAILHVHAKNKPLDETVDLKAISQRTPGFSGADLENLLNEASLIAVREGKKK
                     IDMRDIEEATDRVIAGPAKKSRVISKKERNIVAHHEAGHTIIGMVLDEAEVVHKVTIV
                     PRGQAGGYAMMLPKQDRFLMTEQELLDKICGLLGGRVSEDINFNEVSTGASNDFERAT
                     QIARSMVTQYGMSKKLGPLQFGHSNGQVFLGKDMQGEPNYSSQIAYEIDKEVQRIVKE
                     QYERCKQILLEHKEQLILIAETLLTEETLVAEQIQSLFYEGKLPEIDYDAAKVVKDED
                     SEFNDGKFGKSYEEIRKEQLEDGQRDESEDRKEEKDIAEDKKEADKSDEKDEPAHQQA
                     PNIEKPYDPNHPDNK"
     gene            31426..32307
                     /gene="hslO"
                     /locus_tag="O2701_000350"
     CDS             31426..32307
                     /gene="hslO"
                     /locus_tag="O2701_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCZ4292264.1"
                     /translation="MTHDYIVKALAFDGEIRAYAALTTETVQEAQTRHYTWPTASAAM
                     GRTMTATAMMGAMLKGDQKLTVTVDGQGPIGRIIADANAKGEVRAYVDHPQTHFPLNE
                     QGKLDVRRAVGTNGSIMVVKDVGMKDYFSGASPIVSGELGEDFTYYYATSEQTPSSVG
                     LGVLVNPDNTIKAAGGFIIQVMPGAKDETISKLEKAISEMTPVSKLIEQGLTPEGLLN
                     EILGEDHVQILEKMPVQFECNCSHEKFLNAIKGLGEAEIQNMIKEDHGAEAVCHFCGN
                     KYKYTEEELNVLLESLA"
     gene            32486..33418
                     /gene="cysK"
                     /locus_tag="O2701_000351"
     CDS             32486..33418
                     /gene="cysK"
                     /locus_tag="O2701_000351"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCZ4292265.1"
                     /translation="MAQKPVDNITQIIGGTPVVKLRNVVDDNAADVYVKLEYQNPGGS
                     VKDRIALAMIEKAEREGKIKPGDTIVEPTSGNTGIGLAFVCAAKGYKAVFTMPETMSQ
                     ERRNLLKAYGAELVLTPGSEAMKGAIKKAKELKEEHGYFEPQQFENPANPEVHELTTG
                     PELLQQFEGKTIDAFLAGVGTGGTLSGVGKVLKKEYPNIEIVAIEPEASPVLSGGEPG
                     PHKLQGLGAGFIPGTLNTEIYDSIIKVGNDTAMEMSRRVAKEEGILAGISSGAAIYAA
                     IQKAKELGKGKTVVTVLPSNGERYLSTPLYSFDD"
     gene            33634..34437
                     /gene="folP"
                     /locus_tag="O2701_000352"
     CDS             33634..34437
                     /gene="folP"
                     /locus_tag="O2701_000352"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCZ4292266.1"
                     /translation="MTKTKIMGILNVTPDSFSDGGKFNNVESAINRVKAMIDEGADII
                     DVGGVSTRPGHEMVSLEEEMNRVLPVVEAIVGFDVKISVDTFRSEVAEACLKLGVDMI
                     NDQWAGLYDHRMFQIVAKYDAEIILMHNGNGNRDEPVVEEMLTSLLAQAHQAKIAGIP
                     SNKIWLDPGIGFAKTRNEEAEVMARLDELVATEYPVLLATSRKRFTKEMMGYDTTPVE
                     RDEVTAATTAYGIMKGVRAVRVHNVELNAKLAKGIDFLKENENARHNLS"
     gene            34415..34780
                     /gene="folB"
                     /locus_tag="O2701_000353"
     CDS             34415..34780
                     /gene="folB"
                     /locus_tag="O2701_000353"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HCZ4292267.1"
                     /translation="MQDTIFLKGMRFYGYHGALSAENEIGQIFKVDVTLKVDLAEAGR
                     TDNVIDTVHYGEVFEEVKSIMEGKAVNLLEHLAERIANRINSQYNRVMETKVRITKEN
                     PPIPGHYDGVGIEIVRENK"
     gene            34777..35253
                     /gene="folK"
                     /locus_tag="O2701_000354"
     CDS             34777..35253
                     /gene="folK"
                     /locus_tag="O2701_000354"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCZ4292268.1"
                     /translation="MIQAYLGLGSNIGDRESQLNDAIKILNEYDGINVSNISPIYETA
                     PVGYTEQPNFLNLCVEIQTTLTVLQLLECCLKTEECLHRIRKERWGPRTLDVDILLYG
                     EEMIDLPKLSVPHPRMNERAFVLIPLNDIAANVVEPRSKLKVKDLVFVDDSVKRYK"
     gene            35647..35739
                     /locus_tag="O2701_000355"
     CDS             35647..35739
                     /locus_tag="O2701_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292269.1"
                     /translation="MVNDKVLETSKEMYVEQKCLIFYKTLKENV"
     gene            35792..37279
                     /gene="lysS"
                     /locus_tag="O2701_000356"
     CDS             35792..37279
                     /gene="lysS"
                     /locus_tag="O2701_000356"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCZ4292270.1"
                     /translation="MSEEMNDQMLVRRQKLQELYDLGIDPFGSKFDRSGLSSDLKEEW
                     DQYSKEELVEKEADSHVAIAGRLMTKRGKGKAGFAHVQDLAGQIQIYVRKDQVGDDEF
                     DLWKNADLGDIVGVEGVMFKTNTGELSVKAKKFTLLTKSLRPLPDKFHGLQDIEQRYR
                     QRYLDLITNEDSTRTFINRSKIIQEMRNYLNNKGFLEVETPMMHQIAGGAAARPFVTH
                     HNALDATLYMRIAIELHLKRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAY
                     ADYHDIMDLTESMVRHIANEVLGSAKVQYNGETIDLESAWTRLHIVDAVKEATGVDFY
                     EVKSDEEAKALAKEHGIEIKDTMKYGHILNEFFEQKVEETLIQPTFIYGHPTEISPLA
                     KKNPEDPRFTDRFELFIVGREHANAFTELNDPIDQKGRFEAQLVEKAQGNDEAHEMDE
                     DYIEALEYGMPPTGGLGIGIDRLVMLLTDSPSIRDVLLFPYMRQK"
     gene            37837..37951
                     /gene="rrf"
                     /locus_tag="O2701_000357"
     rRNA            37837..37951
                     /gene="rrf"
                     /locus_tag="O2701_000357"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT        13710 a         5071 c         7648 g        11552 t
ORIGIN      
        1 agaggaaaga agcaagcgat tgtcacaagt caagaaaggt tcttagcgag gatggtagct
       61 aacttacgtt ccgctagagt agaacgttgc tagacaagaa atgaatgcga tgagccgcat
      121 tgagtgtgaa attgatattt taataatgtg cacttttgat tatttaaatc tgtgactaga
      181 agtataagtg aagacattca gaagtattat aaaaagtgaa cagcagtaag atagttttta
      241 atcataaatc atcttactgc tgtttttaga ttttatgtct aatatctttt aaatcgaagt
      301 acaaaaagga aattaattat tatacaatag acaagctatt gcataagtaa cactaacttt
      361 tgtcaaagaa gtgttactat ataattaata cttttgaaag taactaattc caaaacagtg
      421 taaataaagg aagcgtatat catgaagcaa cctattttaa ataaattaga aagtttaaat
      481 caagaagaag cgatttcttt gcatgttccg ggtcataaaa atatgactat cggtcattta
      541 tctcaattat caatgacaat ggataaaact gaaatacctg gattagatga tttacatcat
      601 cctgaagaag tcattttgga aagtatgaag caggtggaga aacattcaga ttatgatgct
      661 tatttcttag tgaatggcac cacttcagga atattatctg tcatccagtc tttttcacag
      721 aaaaaaggcg atatcttaat ggcaagaaat gtacataaat ctgtgttaca tgcgctcgat
      781 attagccaac aagaagggca ttttattgaa acgcatcaaa gtccgttaac gaatcattat
      841 aataaagtta atttaagccg tttgaataat gacggtcaca aacttgctgt gttgacttat
      901 cctaactatt acggtgaaac atttaatgta gaagaggtta tcaaattttt gcaccaatta
      961 aatattcctg tactcattga cgaagcacac ggcgcgcact ttggattgca aggatttcca
     1021 gattctacat taaattatca agctgactat gttgttcaat cttttcataa aacgttacca
     1081 gctttaacga tgggctcggt actttatatt cataaaaatg caccttatag agaaactatt
     1141 atagaatatc taagctactt ccaaacatct agtccttcgt atttgattat ggctagttta
     1201 gagtcagctg ccgagttcta taaaacatat gatagtaccg tgttttttga taagagagcg
     1261 caattaatcg aatgtttgga gaagaagggt tttgaaatgc ttcaagttga tgatccgttg
     1321 aagttgctga taaaatatga aggttttaca ggtcatgata ttcaaaattg gtttatgaat
     1381 gcacatatct atttagaatt agcggacgac tatcaagcat tagcgatatt gccgttatgg
     1441 catcatgatg atacgtattt atttgattcg cttttacgta aaattgaaga tatgatttta
     1501 ccgaaaaaat cagtttctaa agttaaacaa acacaacttt taacaactga aggtaactat
     1561 aaaccaaaac gctttgaata tgttacttgg tgtgatttga aaaaggcaaa aggtaaagtt
     1621 ctggcgcgac atattgtccc gtatccgcca gggattccta ttattttcaa aggagaaaca
     1681 ataactgaaa atatgataga attggtaaat gaatatctgg aaactggaat gatagttgaa
     1741 ggaattaaaa ataataaaat tttagttgag gatgaataaa atgtcagctt ttataacttt
     1801 tgagggccca gaaggctctg gaaaaacaac tgtaattaat gaagtttacc atagattagt
     1861 aaaagattat gatgtcatta tgactagaga accaggtggt gttcctactg gtgaagaaat
     1921 acgtaaaatt gtattagaag gcaatgatat ggacattaga actgaagcaa tgttatttgc
     1981 tgcatctaga agagaacatc ttgtattaaa ggtcatacca gctttaaaag aaggtaaggt
     2041 tgtgttgtgt gatcgctata tcgatagttc attagcttat caaggttatg ctagagggat
     2101 tggcgttgaa gaagtaagag cattaaacga atttgcaata aatggattat atccagactt
     2161 gacgatttat ttaaatgtta gtgctgaagt aggtcgcgaa cgtattatta aaaattcaag
     2221 agatcaaaat agattagatc aagaagattt aaagtttcac gaaaaagtaa ttgaaggtta
     2281 ccaagaaatc attcataatg aatcacaacg gttcaaaagc gttaatgcag atcaacctct
     2341 tgaaaatgtt gttgaagaca cgtatcaaac tatcatcaaa tatttagaaa agatatgata
     2401 taattgttag aagaggtgtt ataaaatgaa aatgattata gcgatcgtac aagatcaaga
     2461 tagtcaggaa cttgcagatc aacttgttaa aaataacttt agagcaacaa aattggcaac
     2521 aacaggtggg tttttaagag cgggtaatac aacattctta tgtggtgtca atgatgaccg
     2581 tgtagatgaa atattgtctg tgattaatca aacgtgtggt aatagagaac agttggtttc
     2641 acctattaca cctatgggag gcagtgcgga ttcgtacatt ccatatccag ttgaagttga
     2701 agttggcggt gctactgtat ttgttatgcc agttgatgca ttccatcaat tttaattcta
     2761 taatacaatc atcaattaga gatacttaaa atagtgtatt aataagttat taacaatttt
     2821 gggttgcttg actgcgacta gttcagatgc caatagattt gatttttgtg gttctaaaaa
     2881 taatcacaaa tcatggtcgc tattgttgca gtaattagtt gctctttggc aaccttttta
     2941 tataaaagca aaagggagtt tgtaatgaat ggatgaacag caacaattga cgaatgcata
     3001 tcattcaaat aaattatcgc atgcctattt atttgaaggt gatgatgcac aaacgatgaa
     3061 acaagttgcg attaattttg caaagcttat tttatgtcaa acagatagtc aatgtgaaac
     3121 aaaggttagt acatataatc atccagactt tatgtatata tcaacaactg agaatgcaat
     3181 taagaaagaa caagttgaac aacttgtgcg tcatatgaat caacttccta tagaaagcac
     3241 aaataaagtg tacatcattg aagactttga aaagttaact gttcaagggg aaaacagtat
     3301 cttgaaattt cttgaagaac caccggacaa tacgattgct attttattgt ctacaaaacc
     3361 tgagcaaatt ttagacacaa tccattcaag atgtcagcat gtatatttca agcctattga
     3421 taaagaaaag tttataaata gattagttga acaaaacatg tctaagccag tagctgaaat
     3481 gattagtact tatactacgc aaatagataa tgcaatggct ttaaatgaag aatttgattt
     3541 attagcatta aggaaatcag ttatacgttg gtgtgaattg ttgcttacta ataagccaat
     3601 ggcacttata ggtattattg atttattgaa acaggctaaa aataaaaaac tgcaatcttt
     3661 aactattgca gctgtgaatg gtttcttcga agatatcata catacaaagg taaatgtaga
     3721 ggataaacaa atatatagtg atttaaaaaa tgatattgat caatatgcgc aaaagttgtc
     3781 gtttaatcaa ttaattttga tgtttgatca actgacggaa gcacataaga aattgaatca
     3841 aaatgtaaat ccaacgcttg tatttgaaca aatcgtaatt aagggtgtga gttagatgcc
     3901 aaatgtaata ggtgttcagt ttcaaaaagc gggaaaatta gaatattata cacctaatga
     3961 tatacaagta gatatagaag actgggtagt tgtcgaatct aaaagaggca tagagatagg
     4021 tattgttaaa aatccattaa tggatattgc tgaagaggat gttgtgttac ctcttaaaaa
     4081 tattattcgc attgctgatg acaaagatat tgataaattt aattgtaatg aacgagatgc
     4141 tgaaaatgca ttaatactat gtaaagacat tgtaagagaa caaggtttgg acatgcgttt
     4201 agtcaattgc gaatatacat tagataaatc gaaagttatt tttaatttta cggcggatga
     4261 tcgtattgat tttagaaaat tagtaaaaat attagcgcaa catttaaaaa cacgtatcga
     4321 gttgagacaa attggtgtaa gggatgaagc caaattgctt ggcggtatcg gaccttgtgg
     4381 taggtcgtta tgttgttcta catttttagg ggattttgaa ccagtatcga ttaagatggc
     4441 taaggatcaa aatttatcat taaatccaac taaaatttct ggtgcatgtg gtcgtttgat
     4501 gtgttgttta aaatatgaaa atgactatta tgaggaagta cgtgcacaat tacctgatat
     4561 tggtgaagca attgaaacgc ctgatggtaa cgggaaagta gttgctttaa atatattaga
     4621 catttctatg caggtgaagc ttgagggaca tgaacagcca cttgaatata aattagaaga
     4681 aatagaaact atgcattaag gaggcattat tacatttgga tcgcaatgaa atatttgaaa
     4741 aaataatgcg tttagaaatg aatgtcaatc aactttcaaa ggaaacttca gaattaaagg
     4801 cacttgcagt tgaattagta gaagaaaatg tagcgcttca acttgaaaat gataatttga
     4861 aaaaggtgtt gggcaatgat gaaccaacta ctattgatac tgcgaattca aaaccagcaa
     4921 aagctgtgaa aaagccatta ccaagtaaag ataatttggc tatattgtat ggagaaggat
     4981 ttcatatttg taaaggcgaa ttatttggaa aacatcgaca tggtgaagat tgtctgttct
     5041 gtttagaagt tttaagtgat taatcaagca cactcaaata gtgttataat tataaatgaa
     5101 tatggtttgg ataagtctga gacaatgcat gtttcaggct ttaattgtgt ataaagtttt
     5161 ggtgattgca taagagatgg cggtactaaa tgttattatt aagtgtgcac gcagtatcat
     5221 tagttataaa atgtagctgt taaaagtcaa aaatacatcg aatgtagtta ggcatataat
     5281 ataaaaagag ttttcaatta ctcaatagaa aaaggttgtc ttcataggag ttaaaaatgt
     5341 taaaagagaa tgaacgattt gatcaactaa tcaaagaaga ttttagtatt attcaaaatg
     5401 atgatgtttt ttcattttca acggatgctt tgttgttagg gcattttaca aaacctagaa
     5461 caaaagatat tgtgttggac ttatgttcag gcaatggggt gatacccttg ttattgtttg
     5521 cgaaacatcc acgacatata gaaggtgttg agattcaaaa aacacttgtc gatatggcgc
     5581 gacgcacatt tcaattcaat gatgttgatg aatatttaac aatgcatcac atggatttga
     5641 aaaacgttac taaagtattt aaaccttcac aatatacttt agtaacgtgt aatccgcctt
     5701 attttaaaga gaatcagcaa caccaacatc aaaaagaagc acataagata gcgagacatg
     5761 agattatgtg tacacttgaa gattgcatga ttgcagcccg tcatttatta aaagaaggtg
     5821 gcaggctaaa catggtacat cgtgcagaga gactaatgga tgtcttgttt gaaatgagaa
     5881 aagtgaatat tgaacctaag aaagtcgttt ttatatatag taaagtaggg aaatcagcac
     5941 aaacgatagt agtagaaggt cgaaaaggtg gaaatcaagg tttagaaatc atgcccccat
     6001 tttatattta taatgaagat ggtaattata gcgaagaaat gaaggaagta tattatggat
     6061 agtcattttg tatatattgt aaaatgtagt gatggaagtt tatatacagg atacgctaaa
     6121 gacgttaatg cacgtgttga aaaacataac cgaggtcaag gagccaaata tacgaaagta
     6181 agacgtccgg tgcatttagt ttatcaagaa atgtatgaga caaagtctga agcattgaag
     6241 cgtgaatatg aaattaaaac ttataccaga caaaagaaat tgcgattaat taaggagcga
     6301 tagtatggct gtattatatt tagtgggcac accaattggt aatttagcag atattactta
     6361 tagagcagtt gatgtattga aacgtgttga tatgattgct tgtgaagaca ctagagtaac
     6421 tagtaaactg tgtaatcatt atgatattcc aactccatta aagtcatatc acgaacataa
     6481 caaggataag cagactgctt ttatcattga acagttagaa ttaggtcttg acgttgcgct
     6541 cgtatctgat gctggattgc ccttaattag tgatcctgga tacgaattag tagtggcagc
     6601 cagagaagct aatattaaag tagagaccgt gcctggacct aatgctgggc tgacggcttt
     6661 gatggctagt ggattacctt catatgtata tacattttta ggatttttgc cacgaaaaga
     6721 gaaagaaaaa agtgctgtat tagagcaacg tatgcatgaa aatagcacat taattatata
     6781 cgaatcaccg catcgtgtga cagatacatt aaaaacaatt gcaaagatag atgcaacacg
     6841 acaagtatca ctagggcgtg aattaactaa gaagttcgaa caaattgtaa ctgatgatgt
     6901 aacacaatta caagcattga ttcagcaagg cgatgtacca ttgaaaggcg aattcgttat
     6961 cttaattgaa ggtgctaaag cgaacaatga gatatcgtgg tttgatgatt tatctatcaa
     7021 tgagcatgtt gatcattata ttcaaacttc acagatgaaa ccaaaacaag ctattaaaaa
     7081 agttgctgaa gaacgacaac ttaaaacgaa tgaagtatat aatatttatc atcaaataag
     7141 ttaatcactt tatcgattat atgaaatttt aaacgatttt ataaacgcaa gctgtaattt
     7201 taaatggtaa gttatcattt tgcattgata ctgataaaat gatgttgact atgataaaaa
     7261 aatgatgaca tcgacgtttt ttaatgtaaa ataaatacat tgaaagtaat aaatacctta
     7321 acattgaata agatgaaaat gagatgacga gataaatgtt cgcgtccgtt gaaatgcata
     7381 gaaatcttag atattatttg aagtgagaca ttacgaggag gaacagttat ggctaaagaa
     7441 acattttata taacaacccc aatatactat cctagtggga atttacatat aggacatgca
     7501 tattctacag tggctggaga tgttattgca agatataaga gaatgcaagg atatgatgtt
     7561 cgctatttga ctggaacgga tgaacacggt caaaaaattc aagaaaaagc tcaaaaatct
     7621 ggtaagacag aaattgaata tttggatgag atgattgctg gaattaaaca attgtgggct
     7681 aagcttgaaa tttcaaatga tgattttatc agaacaactg aagaacgtca taaacatgtc
     7741 gttgagcaag tgtttgaacg tttattaaag caaggtgata tctatttagg tgaatatgaa
     7801 ggttggtatt ctgttccgga tgaaacatac tatacagagt cacaattagt agacccacaa
     7861 tacgaaaacg gtaaaattat tggtggcaaa agtccagatt ctggacacga agttgaacta
     7921 gttaaagaag aaagttattt ctttaatatt agtaaatata cagaccgttt attagagttc
     7981 tatgaccaaa atccagattt tatacaacca ccatcaagaa aaaatgaaat gattaacaac
     8041 ttcattaaac caggacttgc tgatttagct gtttctcgta catcatttaa ctggggtgtc
     8101 catgttccgt ctaatccaaa acatgttgtt tatgtttgga ttgatgcgtt agttaactat
     8161 atttcagcat taggctattt atcagatgat gagtcactat ttaacaaata ctggccagca
     8221 gatattcatt taatggctaa ggaaattgtg cgattccact caattatttg gcctatttta
     8281 ttgatggcat tagacttacc gttacctaaa aaagtctttg cacatggttg gattttgatg
     8341 aaagatggaa aaatgagtaa atctaaaggt aatgtcgtag accctaatat tttaattgat
     8401 cgctatggtt tagatgctac acgttattat ctaatgcgtg aattaccatt tggttcagat
     8461 ggcgtattta cacctgaagc atttgttgag cgtacaaatt tcgatctagc aaatgactta
     8521 ggtaacttag taaaccgtac gatttctatg gttaataagt actttgatgg cgaattacca
     8581 gcgtatcaag gtccacttca tgaattagat gaagaaatgg aagctatggc tttagaaaca
     8641 gtgaaaagct acactgaaag catggaaagt ttgcaatttt ctgtggcatt atctacggta
     8701 tggaagttta ttagtagaac gaataagtat attgacgaaa caacgccttg ggtattagct
     8761 aaggacgata gccaaaaaga tatgttaggc aatgtaatgg ctcacttagt tgaaaatatt
     8821 cgttatgcag ctgtattatt acgtccattc ttaacacatg cgccgaaaga gatttttgaa
     8881 caattgaaca ttaacaatcc tcaatttatg gaatttagta gtttagagca atatggtgtg
     8941 cttaatgagt caattatggt tactgggcaa cctaaaccta ttttcccaag attggatagc
     9001 gaagcggaaa ttgcatatat caaagaatca atgcaaccgc ctgctactaa agaggaaaaa
     9061 gaagagattc ctagcaaacc tcaaattgat attaaagact ttgataaagt tgaaattaag
     9121 gcagcaacga ttattgatgc tgaacatgtt aagaagtcag ataagctttt aaaaattcaa
     9181 gtagacttag attctgaaca aagacaaatt gtatcaggaa ttgccaaatt ctatacacca
     9241 gatgatatta ttggtaaaaa agtagcagtt gttactaacc tgaaaccagc taaattaatg
     9301 ggacaaaaat ctgaaggtat gatattatct gctgaaaaag atggtgtatt aaccttagta
     9361 agtttaccaa gtgcaattcc aaatggtgca gtgattaaat aactgtattt ttaaaaatta
     9421 ggagagataa ttatgttaat cgatacacat gtccatttaa atgatgagca atacgatgat
     9481 gatttgagtg aagtgattac acgtgctaga gaagcaggtg ttgatcgtat gtttgtagtt
     9541 ggttttaaca aatcgacaat tgaacgcgcg atgaaattaa tcgatgagta tgatttttta
     9601 tatggcatta tcggttggca tccagttgac gcaattgatt ttacagaaga acacttggaa
     9661 tggattgaat ctttagctca gcatccaaaa gtgattggta ttggtgaaat gggattagat
     9721 tatcactggg ataaatctcc tgcagatgtt caaaaggaag tttttagaaa gcaaattgct
     9781 ttagctaagc gtttgaagtt accaattatc attcataacc gtgaagcaac tcaagactgt
     9841 atcgatatct tattggagga gcatgctgaa gaggtaggcg ggattatgca tagctttagt
     9901 ggttctccag aaattgcaga tattgtaact aataagctga atttttatat ttcattaggt
     9961 ggacctgtga catttaaaaa tgctaaacag cctaaagaag ttgctaagca tgtgtcaatg
    10021 gagcgtttgc tagttgaaac cgatgcaccg tatctttcgc cacatccgta tagagggaag
    10081 cgaaatgaac cggcgagagt aactttagta gctgaacaaa ttgctgaatt aaaaggctta
    10141 tcttatgaag aagtgtgcga acaaacaact aaaaatgcag agaaattgtt taatttaaat
    10201 tcataaagtt aaaagtgaga aagatcaccg ccataaatgt aaacgatgct atattcgttt
    10261 aatatgctat ggttctttct cactttttta aattaaaata tcgtgcatgt ggaatacgtg
    10321 cgatagagat ggttagagct ttgaaattaa gaattgtagg aaggcgtttt aaatgaaaat
    10381 caatgagttt atagttgtag aaggacgaga tgatactgag cgtgttaaac gagctgttga
    10441 atgtgatacg attgaaacga atggtagtgc catcaacgaa caaactttag aagtaattag
    10501 aaatgctcaa caaagtcgag gcgttattgt attaacagat ccagatttcc caggagataa
    10561 aattagaagt acaattactg aacatgtcaa aggtgttaaa catgcgtata ttgatagaga
    10621 aaaagctaaa aataaaaaag ggaaaattgg tgttgaacat gccgaattaa ttgatattaa
    10681 agaagcgtta atgcatgtta gttcaccctt tgatgaagct tatgaatcaa ttgataaatc
    10741 tgtgctaata gagttggggt taattgttgg gaaagatgca aggcgccgta gagaaatttt
    10801 aagtagaaaa ttgcgaatcg gccattccaa tggtaagcag ttattgaaaa agttaaatgc
    10861 atttggttat accgaagcgg atgtaaggca agctttagaa gatgaatgag gaagtgaaaa
    10921 tgttggataa taaagatatt gcaacaccat caagaacgcg agcgttgtta gataaatatg
    10981 gctttaattt taaaaaaagt ttaggacaga actttttgat agatgtgaat atcattaata
    11041 atatcattga tgcaagtgat attgatgcac aaactggggt gattgaaatt ggtccaggca
    11101 tggggtcatt gacagaacaa ttggccagac atgctaaaag agtattggca tttgaaattg
    11161 atcaacgttt aatacctgta ttaaatgata cactatcacc ttatgataat gtgacggtga
    11221 ttaatgaaga tattttaaaa gcgaatatta aagaagctgt tgaaaatcat ttacaagatt
    11281 gtgaaaaaat aatggttgtt gcaaacctgc cgtactatat tacgacgcca attttattaa
    11341 atttgatgca acaagatata ccaattgatg gctacgtggt gatgatgcaa aaagaagtgg
    11401 gcgaacgctt aaatgctgaa gtaggttcaa aagcatatgg ttcgttatca attgtcgtac
    11461 aatactatac agagactagt aaagtattaa cggtacctaa atctgtattt atgccaccac
    11521 ctaatgttga ttcaatagtt gtaaaactga tgcagagaac tgaaccgtta gtaacagtag
    11581 ataacgagga agcattcttt aagttagcaa aagcagcatt tgcacaaaga agaaagacaa
    11641 ttaacaataa ctatcaaaat tattttaaag atggtaaaca acacaaagaa gtgattttac
    11701 aatggttgga acaagcaggt attgatccaa gacgtcgcgg tgaaacgcta tctattcaag
    11761 attttgctaa attgtatgaa gaaaagaaaa aattccctca attagaaaat taaatgattg
    11821 acaaagcaaa gcactattgt taaaatttaa attttgtttg acgaaaacgt tgcaaatatg
    11881 gtattatgta acttgtagcg aggtggagca atatgccaaa atcaattttg gacatcaaaa
    11941 attctattga ttgtcatgta ggaaatcgta ttgtactgaa agccaatgga ggccgtaaga
    12001 aaacaataaa acgttctgga attttaaaag aaacatatcc gtcagttttc attgttgagt
    12061 tagatcaaga caaacacaac tttgagagag tatcttatac atacactgat gtgttaactg
    12121 aaaatgttca agtttcattt gaagaggata atcatcacga atcaattgca cactaaataa
    12181 gacatataga gatgttagac gtttcttagt ataagaagta aatattatga taattatttg
    12241 agtgttgggc attatgttca atactctttt tatttacaaa atgtttaaca ctgatgtttc
    12301 gcttatagat ttttcagtaa atggataatt gtatttataa acacaaatac aagtaaatac
    12361 taagtaatta gatggagaaa attacttttt tattaaaaaa acactaaaaa acaaattaaa
    12421 atgtcaaata ttaattctct ttatgttaaa atcatcatat taagataacg aaaagagggc
    12481 ggaaaatgat atatgaaacg gcaccagcca aaattaattt tacgctcgat acacttttta
    12541 aaagaaatga tggctatcat gagattgaaa tgataatgac aacagttgat ttaaatgatc
    12601 gtttaacttt tcataaaaga aaagatcgaa agatagttgt tgagattgaa cataattatg
    12661 tgccttctaa tcataaaaat ctcgcatatc gtgcagcgca actatttatt gagcaatatc
    12721 aactaaagca aggtgtaaca atttctatcg ataaagaaat acctgtttct gctggcttag
    12781 ctggaggttc ggctgatgca gcagcaacgt taagaggatt gaatcgactt tttgatatag
    12841 gggcgagttt ggaagaattg gctctactag gcagtaaaat cgggacagat attccgtttt
    12901 gtatttataa taaaactgca ctatgtactg gaagaggaga gaaaatcgag tttttaaata
    12961 aaccaccttc agcttgggtg attcttgcta aaccaaactt aggcatatca tcaccagata
    13021 tatttaagtt gattaattta gataagcgtt acgacgtaca tacgaaaatg tgttatgagg
    13081 ccttagaaaa tcgagattat caacaattat gtcaaagttt gtctaatcga ttagagccaa
    13141 tttctgtttc aaaacaccca caaatcgata aattaaaaaa taatatgttg aaaagtggtg
    13201 cagatggtgc gttaatgagt ggaagcggac ctactgtgta tgggctagca cgaaaagaaa
    13261 gccaagcaaa aaatatttat aatgcagtta acggttgttg taatgaagtg tacttagtta
    13321 gactattagg atagaagggt tgaaaagatg agatataaac gaagcgagag aattgttttt
    13381 atgacgcaat atttgatgaa ccatccgaat aaattgattc cattaacttt ttttgtgaaa
    13441 aaatttaaac aggcgaagtc ttcaataagt gaagatgtcc aaattataaa aaatacattc
    13501 caaaaagaaa agttaggtac agtaattact actgctggcg caagtggtgg tgttacgtat
    13561 aaaccaatga tgagtaaaga agaggcgact gaagttgtta atgaggtcat tactctatta
    13621 gaagagaaag aacgtttgtt acctggcgga tatttatttt tatcagattt ggtaggtaat
    13681 ccatcgctac taaacaaagt tggtaagtta attgccagta tttacatgga agaaaaatta
    13741 gatgctgttg ttaccattgc gacaaaaggt atttcattgg caaatgcggt tgctaatatt
    13801 ttaaatttac cagtagtagt gattagaaaa gacaacaagg tgactgaagg ttctacagtt
    13861 tcaattaatt acgtttcagg atcttcaaga aaaatagaaa caatggtact ttcgaagaga
    13921 actttagcag aaaattcaaa tgttttagtt gtcgatgatt ttatgagggc tggtggctct
    13981 attaatggtg ttatgaattt aatgaatgag tttaaagccc atgtaaaagg ggtatcagta
    14041 cttgtagaat caaaagaagt taaacaaaga ttgattgaag attatacttc cttagtgaaa
    14101 ttatctgatg tagatgaata taatcaagag tttaacgtag aacctggcaa cagtttatct
    14161 aagttttcat aaaaggagtt ttagtattat gaaaatcatt aacacaacaa gattaccgga
    14221 agcacttgga ccatattcgc atgcaacagt tgtgaatggt atggtttata cttctggtca
    14281 gattccattg aatattgatg gacatatcgt aagcgctgat gttcaagcac agacaaaaca
    14341 agttttagaa aatttaaagg ttgttttgga agaagcagga tctgatttga attctgttgc
    14401 gaaagcgacc attttcatta aagatatgaa tgatttccaa aaaataaatg aagtgtatgg
    14461 tcaatatttt aatgaacaca agccagcgcg tagttgtgta gaggttgcgc gtttgccaaa
    14521 agatgtgaaa gtagaaattg aattagtaag taaaattaag gaattataat tttcgattaa
    14581 tatgtttaat caagcttcta aataaaacag agagatatat actatagggg ggctcactac
    14641 atgaaagtga cagatgtaag acttagaaaa atacaaacag atggacgaat gaaagcactc
    14701 gtttccatta cattagatga agctttcgta attcatgatt tacgtgtaat tgaaggaaac
    14761 tctggcttgt tcgttgcaat gccaagtaaa cgtacaccag atggtgaatt ccgcgacatc
    14821 gcgcatccta ttaattcaga tatgagacaa gaaattcaag atgcagtgat gaaagtatat
    14881 gatgaaacag atgaagtagt accagataaa aacgctacat cagaagattc agaagaagct
    14941 taatcaattt tatatttagc gatgtaatac atttgcaata agttgatttg atactgtcga
    15001 taaagcataa agctttgtcg gcagtttttt tagtttgtat taatgttttt ttatttttaa
    15061 tgaaaggcta ataaatatat acgttaacag attatgatga tatgaaaatt attgattcat
    15121 tgctgataaa aattgcgttt gaatgatgct cgtatttttg aagtaagaaa aaagttgttt
    15181 ttaaaattac aacgaattaa aaacaatgcc ttttatatgt tgaaagagta ttgcagatta
    15241 aattataata atgacgaagt gtaaaattta atgggggtta atgttcatgc gaagacacgc
    15301 gataattttg gcagcaggta aaggcacaag aatgaaatct aaaaagtata aagtgctaca
    15361 cgaggttgct gggaaaccta tggtcgaaca tgtattggaa agtgtgaaag gctctggtgt
    15421 cgatcaagtt gtaaccatcg taggacatgg tgctgaaagt gtaaaaggac atttaggcga
    15481 gcgttcttta tacagttttc aagaggaaca actcggtact gcgcatgcag tgcaaatggc
    15541 gaaatcacac ttagaagaca aggaaggtac gacaatcgtt gtatgtggtg acacaccgct
    15601 catcacaaag gaaacattag aaacattgat tgcgcatcac gaggatgcta atgctcaagc
    15661 aactgtatta tctgcatcga ttcaacaacc atatggatac ggaagaatcg ttcgaaatgc
    15721 gtcaggtcgt ttagaacgca tagttgaaga gaaagatgca acgcaagctg aaaaggatat
    15781 taatgaaatt agttcaggta tttttgcgtt taataataaa acgttgtttg aaaaattaac
    15841 acaagtgaaa aatgataatg cgcaaggtga atattacctc cctgatgtat tgtcgttaat
    15901 tttaaatgat ggcggcatcg tagaagtcta tcgtaccaat gatgttgaag aaatcatggg
    15961 tgtaaatgat cgtgtaatgc ttagtcaggc tgagaaggcg atgcaacgtc gtacgaatca
    16021 ttatcacatg ctaaatggtg tgacaatcat cgatcctgac agcacttata ttggtccaga
    16081 cgttacaatt ggtagtgata cagtcattga accaggcgta cgaattaatg gtcgtacaga
    16141 aattggcgaa gatgttgtta ttggtcagta ctctgaaatt aacaatagta cgattgaaaa
    16201 tggtgcatgt attcaacagt ctgttgttaa tgatgctagc gtaggagcga atactaaggt
    16261 cggaccgttt gcgcaattga gaccaggcgc gcaattaggt gcagatgtta aggttggaaa
    16321 ttttgtagaa attaaaaaag cagatcttaa agatggtgcc aaggtttcac atttaagtta
    16381 tattggcgat gctgtaattg gcgaacgtac taatattggt tgcggaacga ttacagttaa
    16441 ctatgatggt gaaaataaat ttaaaactat cgtcggcaaa gattcatttg taggttgcaa
    16501 tgttaattta gtagcacctg taacaattgg tgatgatgta ttggtggcag ctggttccac
    16561 aatcacagat gacgtaccaa atgacagttt agctgtggca agagcaagac aaacaacaaa
    16621 agaaggatat aggaaataat catttacgta tttaaaatgg ctaggataaa aggataatcc
    16681 tatgtaatat taatgtaatc tttatgattt aatgattcgc atagtaatgg agttacattt
    16741 tatatataat agtaattgcg taagtaaata attggaggac tataaatgtt aaataatgaa
    16801 tataagaatt cgtcattaaa gattttttca ttgaaaggaa acgaagcatt agcgcaagaa
    16861 gttgctgacc aagtaggaat tgaactaggt aaatgttcag ttaaacgttt tagtgatgga
    16921 gaaattcaaa ttaatatcga agagagtatt cgtggttgtg acgtatttat tattcaacca
    16981 acatcatatc ctgtgaatct acatttaatg gaattattaa ttatgattga tgcttgtaaa
    17041 cgtgcttctg cagcaacaat caatattgta gtgccatatt atggatatgc aagacaagat
    17101 agaaaagccc gtagccgtga gccaatcact gctaaattag ttgcaaactt aatcgaaaca
    17161 gctggcgcaa ctcgtatgat tgcgttagac ttacatgcac cacaaattca aggattcttt
    17221 gatattccaa ttgaccactt aatgggtgtg ccaattcttg ctaaacattt caaagatgat
    17281 ccgaatatta acccagaaga atgtgtcgtt gtttcaccag accatggcgg cgttacacgt
    17341 gcacgtaaat tagctgacat tttaaaaact ccaattgcaa ttatagataa acgtcgtcct
    17401 agaccaaatg ttgctgaagt gatgaacatt gttggtgaga ttgaaggacg tacggcaatt
    17461 attattgacg atattattga tacagcaggt acaatcactt tagctgcaca agcattaaaa
    17521 gataaaggtg ctaaagaagt atatgcttgt tgtacacacc ctgttttatc aggaccggct
    17581 aaagaacgta tcgaaaattc tgctataaaa gaattaatcg taacaaactc aattcattta
    17641 gatgaagatc gcaaaccatc taacactaaa gaattatctg ttgctggttt aatcgcacaa
    17701 gctatcattc gtgtatacga aagagaatca gttagcgtat tatttgacta atatttaaaa
    17761 ggcgtttgac gaacatattc caaacgtgta taatagtttc gttcgtgatt atacgaataa
    17821 ataaacactt gcaagcaacg atgatgttga tgggtaagtg aggtgctcgt tttgagcaaa
    17881 aatgaaaggt ggaaatgaga atggcttcat taaagtcaat catccgtcaa ggtaaacaaa
    17941 cacgttcaga tcttaaacaa ttaagaaaat ctggtaaagt accagcagta gtatacggtt
    18001 acggtactaa aaacgtgtca gttaaagttg atgaagtaga attcatcaaa gttatccgtg
    18061 aagtaggtcg taacggtgtt atcgaattag gcgttggttc taaaactatc aaagttatgg
    18121 ttgcagacta ccaattcgat ccacttaaaa accaaattac tcacattgac ttcttagcaa
    18181 tcaatatgag tgaagaacgt actgttgaag taccagttca attagttggt gaagcagtag
    18241 gcgctaaaga aggcggcgta gttgaacaac cattattcaa cttagaagta actgctactc
    18301 cagacaatat tccagaagca atcgaagtag acattactga attaaacatt aacgacagct
    18361 taactgttgc tgatgttaaa gtaactggcg acttcaaaat cgaaaacgat tcagctgaat
    18421 cagtagtaac agtagttgct ccaactgaag aaccaactga agaagaaatc gaagctatgg
    18481 aaggcgaaca acaaactgaa gaaccagaag ttgttggcga aagcaaagaa gacgaagaaa
    18541 aaactgaaga gtaattttaa tctgttacat taaagttttt atactttgtt taacaagcac
    18601 tgtgcttatt ttaatataag catggtgctt tttgtgttat tataaagctt aattaaactt
    18661 tattactttg tactaaagtt taattaattt tagtgagtaa aagacattaa actcaacaat
    18721 gatacatcat aaaaatttta atgtactcga ttttaaaata catacttact aagctaaaga
    18781 ataatgataa ttgatggcaa tggcggaaaa tggatgttgt cattataata ataaatgaaa
    18841 caattatgtt ggaggtaaac acgcatgaaa tgtattgtag gtctaggtaa tataggtaaa
    18901 cgttttgaac ttacaagaca taatatcggc tttgaagtcg ttgattatat tttagagaaa
    18961 aataattttt cattagataa acaaaagttt aaaggtgcat atacaattga acgaatgaac
    19021 ggcgataaag tgttatttat cgaaccaatg acaatgatga atttgtcagg tgaagcagtt
    19081 gcaccgatta tggattatta caatgttaat ccagaagatt taattgtctt atatgatgat
    19141 ttagatttag aacaaggaca agttcgctta agacaaaaag gaagtgcggg cggtcacaat
    19201 ggtatgaaat caattattaa aatgcttggt acagaccaat ttaaacgtat tcgtattggt
    19261 gtgggaagac caacgaatgg tatgacggta cctgattatg ttttacaacg cttttcaaat
    19321 gatgaaatgg taacgatgga aaaagttatc gaacacgcag cacgcgcaat tgaaaagttt
    19381 gttgaaacat cacgatttga ccatgttatg aatgaattta atggtgaagt gaaataatga
    19441 caatattgac aacgcttata aaagaagata atcattttca agaccttaat caggtatttg
    19501 gacaagcaaa cacactagta actggtcttt ccccgtcagc taaagtgacg atgattgctg
    19561 aaaaatatgc acaaagtaat caacagttat tattaattac caataattta taccaagcag
    19621 ataaattaga aacagattta cttcaattta tagatgctga agaattgtat aagtatcctg
    19681 tgcaagatat tatgaccgaa gagttttcaa cacaaagccc tcaactgatg agtgaacgta
    19741 ttagaacttt aactgcgtta gctcaaggta agaaagggtt atttatcgtt cctttaaatg
    19801 gtttgaaaaa gtggttaact cctgttgaaa tgtggcaaaa tcaccaaatg acattgcgtg
    19861 ttggtgagga tatcgatgtg gaccaatttc ttaacaaatt agttaatatg gggtacaaac
    19921 gggaatccgt ggtatcgcat attggtgaat tctcattgcg aggaggtatt atcgatatct
    19981 ttccgctaat tggggaacca atcagaattg agctatttga taccgaaatt gattctattc
    20041 gggattttga tgttgaaacg cagcgttcca aagataatgt tgaagaagtc gatatcacaa
    20101 ctgcaagtga ttatatcatt actgaagaag tgatcagcca tcttaaagaa gagttaaaaa
    20161 ctgcatatga aaatacaaga cccaaaatag ataaatcagt gcgcaatgat ttgaaagaaa
    20221 cgtatgaaag ctttaaatta ttcgaaagta catactttga tcatcaaata ctacgtcgct
    20281 tagtagcgtt tatgtatgaa acaccttcga caattattga gtatttccaa aaagatgcaa
    20341 tcattgcagt tgatgaattt aatcgtatta aagaaactga agaaagttta acagtagagt
    20401 ctgattcgtt tattagcaat attattgaaa gtggtaatgg atttatagga caaagtttta
    20461 taaaatatga tgattttgaa acattgattg aaggctatcc tgtcacttat ttttcattat
    20521 tcgctacaac aatgccgata aaactaaatc atattattaa attttcatgt aaacctgtcc
    20581 aacaatttta tgggcaatat gacattatgc gttctgaatt tcaacgatat gttaatcaaa
    20641 actatcatat cgtggttttg gtcgaaaccg aaactaaagt tgaacgtatg caagcgatgt
    20701 taagtgaaat gcatattcca tcaataacaa aattgcatcg ctcaatgtca tcggggcaag
    20761 cagtgattat tgaaggcagt ttatctgaag gatttgaact acctgatatg ggattagttg
    20821 tcattactga gcgtgagctt tttaaatcaa aacagaaaaa gcaacgaaaa cgtacgaaag
    20881 ctatctcaaa tgctgaaaaa attaagtctt accaagattt aaatgtggga gattatattg
    20941 ttcatgtgca tcatggtgtt ggtagatatt taggtgttga gacgctcgaa gtggggcaaa
    21001 cgcatcgtga ttatattaaa ttgcaatata aaggtacgga tcaactattt gttccagtag
    21061 atcaaatgga tcaagttcaa aaatatgtag cttcggaaga taagacgcca aaattaaata
    21121 aactcggtgg cagtgaatgg aaaaaaacaa aagctaaagt tcaacaaagt gttgaagata
    21181 ttgctgaaga gttgattgat ttatataaag aaagagaaat ggcagaaggt tatcaatatg
    21241 gggaagacac agctgagcaa acaacatttg aattagattt tccatatgaa cttacgcctg
    21301 accaagctaa atctatcgat gaaattaaag atgacatgca aaaatcgcgt ccaatggatc
    21361 gcttgctatg tggtgatgtt ggttatggta aaactgaagt tgcagtgaga gcagcattca
    21421 aagctgtaat ggaaggaaag caggttgcat ttttagttcc tacaactatt ttagctcagc
    21481 aacattatga gacgttaatt gagcgtatgc aagattttcc tgttgaaatt caattaatga
    21541 gtcgttttag aacgcctaaa gagataaaac aaactaagga aggacttaaa actggatttg
    21601 ttgacatagt tgttggtaca cacaaattac ttagtaaaga tatacagtat aaagatttag
    21661 ggctgttgat tgtagatgaa gaacaacgat ttggtgtacg ccataaagag cgtattaaaa
    21721 cattaaaaca taatgtagat gtactaacat tgactgcaac cccaatacct agaacattgc
    21781 atatgagtat gctaggtgtg cgggatttgt cagtgattga aacgccgcca gaaaatcgtt
    21841 tcccagttca aacatatgta ttagaacaga acatgagttt tatcaaagaa gctttagaaa
    21901 gagaactatc ccgtgatggc caagtgtttt atctttataa taaagtgcaa tccatttatg
    21961 aaaaacgaga acaactccag atgttaatgc cagatgctaa cattgcagtt gctcatggac
    22021 aaatgacaga gcgcgattta gaagaaacga tgttaagttt tatcaataat gaatatgata
    22081 ttttagtaac gacgacgatt attgaaacag gtgtcgatgt cccaaatgca aatactttga
    22141 tcattgaaga tgcagatcgc tttggattga gtcagttgta tcaattaaga ggtcgtgttg
    22201 gtcgttcaag tcgtattggt tatgcatact tcttacatcc agcaaataag gtactaactg
    22261 agactgcaga agatcgatta caagcgatta aagaatttac ggagttaggc tcaggattta
    22321 agattgcgat gcgtgatttg aacattcgtg gtgctggtaa tttgttaggt aaacaacagc
    22381 acggctttat tgatacagtt ggatttgatt tgtacagtca aatgttagaa gaagctgtaa
    22441 atgaaaaacg tggtattaag gaaccagaat ctgaggtgcc agaagtcgaa gttgatttaa
    22501 acttggatgc atatttgcca acagaatata ttgcaaatga acaagctaaa attgaaattt
    22561 ataaaaagct acgaaaaact gaaacatttg atcaaattat cgacattaaa gatgaattaa
    22621 ttgatcgttt caatgattat cctgttgaag tagcacgttt gcttgatata gtggaaataa
    22681 aagtacacgc attacattca ggtatcacgt tgattaaaga taaagggaaa ataattgata
    22741 ttcatttatc tgtaaaagcc actgaaaata ttgatggcga agtgctgttc aaagcaacac
    22801 aacctttagg tagaacaatg aaggttggtg ttcaaaataa tgcaatgaca attactttaa
    22861 cgaaacaaaa tcaatggctt gatagtttga agtttttagt taagtgcatt gaagaaagta
    22921 tgagaatcag tgatgaagca taaagaagca tttaatggcg ttgtcgtgtt aactgctgca
    22981 ttaattgtca ttaaaattct gagtgctgta tatcgaattc catatcaaaa tatattaggc
    23041 gatacaggtt tgtatgcata tcaacaagtg tatccaattg tagcattagg aatgatatta
    23101 tcgatgaatg ccattcctag tgcaattaca caaaatatag ggaagtatca tagtgacgaa
    23161 gcatatgcaa aagcagtcgc ttatatacaa ttagttggta tattattatt tattgctatt
    23221 tttgtgtttg cgaacaatat tgcacatatg atgggtgatg gccatttaac accaatgatt
    23281 caagctgcaa gtttaagctt tatatttata ggtatgcttg gcgtgttaag aggttattat
    23341 caatctgcaa ataatatgac agttccggct atttcccagg ttatagaaca agttatacga
    23401 gtaggtatta tcattgttac tattgttatt tttgtagaca gaggttggac gatatatgaa
    23461 gcgggaacaa ttgctatttt agcatcaacg ataggttttt taggttcttc aatttattta
    23521 gtagcgcacc gaccttttaa gtttaaaatg gtaaataaca ctgcaaagat cgtttggaaa
    23581 cagttcgcac tttcggtttt gattttcgct atcagtcaat taatcgtaat tttatggcaa
    23641 gtgattgata gtgttactat tattaagtca cttcaagcga tacgcgtgcc attcgatgtt
    23701 gccataactg aaaaaggagt ctatgaccgt ggtgcatcat ttattcagat gggattgatt
    23761 gtaactacaa catttagttt tgcgctcatt cctctgttaa gtgacgcaat taaaatgaat
    23821 aatcaggtac ttatgaatcg ttatgcaaat gcgtcattaa agattacgat tttaataagt
    23881 acagcagcag gaataggatt aattaattta ttgcctttaa tgaacggtgt gttttttaag
    23941 acgaatgatt taaccttaac gttaagtgtt tatatgatta cggtcatttg tgtatcgtta
    24001 attatgatgg atatggcatt attacaagcg caacatgctg tgagacctat ttttgttggt
    24061 atgacggcag gattggttat taaatttata cttaatatca ttttgattcg tttaagtggc
    24121 attattggtg cgagcattag tactgttgta tcattaatta tattcggtac gattatccat
    24181 attgctgtca cgagaaaata ccacttatat gcgatgagac gattttttat caatgttgtt
    24241 ttaggtatgg tatttatgtc gattgttgtt caatgcgtgt taaacatagt gacaacacac
    24301 ggtagaatca ctggactcat tgaattatta tgtgcagcag tattaggtat cattgcattg
    24361 tttttctata tttttagatt taatgttttg acatataaag agttaactta tttaccattt
    24421 ggttcaaagt tgtatcaaat taagaaagga agacgttgat ggcacatacc attacgattg
    24481 ttggcttagg aaactatggc attgatgatt tgccgctagg gatatataaa tttttaaaga
    24541 cacaagataa agtttatgca agaacgttag atcatccagt tatagaatca ttgcaagatg
    24601 aattaacatt tcagagtttt gaccatgttt atgaagcaca taaccaattt gaagatgtct
    24661 atattgatat tgtggcgcaa ttggttgaag ctgctaatga aaaagatatt gtctatgcgg
    24721 ttccgggtca tcctagagtt gctgagacaa ctacagtgaa attactggct ttagcaaagg
    24781 acaatactga tatagatgtg aaagttttag gtgggaaaag ctttattgat gatgtgtttg
    24841 aagcagttaa tgtagatcca aatgatggct tcacactgtt agatgcgaca tcattacaag
    24901 aagtaacact taatgttaga acgcatacat tgattacgca agtttatagt gcaatggttg
    24961 ctgctaattt gaaaatcact ttaatggaac gatatcctga tgattaccct gttcaaattg
    25021 tcactggtgc acgaagcgat ggtgcggata acgttgtgac atgcccatta tatgaattgg
    25081 atcatgatga aaatgcattc aataatttga cgagtgtatt cgtaccaaaa atcataacat
    25141 cgacatattt gtatcatgac tttgattttg caacggaagt gattgatact ttagttgatg
    25201 aagataaagg ttgtccatgg gataaagtgc aaacgcatga aacgctaaag cgttatttac
    25261 ttgaagaaac atttgaattg ttcgaagcta ttgacaatga agatgattgg catatgattg
    25321 aagaactagg agatatttta ttacaagtgt tattgcatac tagtattggt aaaaaagaag
    25381 ggtatatcga cattaaagaa gtgattacaa gtcttaatgc taaaatgatt cgtagacacc
    25441 cacacatatt tggtgatgcc aatgctgaaa ctatcgatga cttaaaagaa atttggtcta
    25501 aggcgaaaga tgctgaaggt aaacagccaa gagttatatt tgaaaaagta tttgcagagc
    25561 attttttaaa tttatatgag aagacgaagg ataagtcatt tgatgaggcc gcgttaaagc
    25621 agtggctaga aaaaggggag agtaatacat gagattagat aaatatttaa aagtatcacg
    25681 gttaataaag cgacgtacgc tagcaaaaga agtaagtgat caaggtagaa ttacaataaa
    25741 tggtaatgtt gctaaagctg gatcggatgt taaagttgaa gatgtgctga cgattcgctt
    25801 tggtcaaaaa ttagtaacag ttaaagtaac tgcattaaat gaacatgcat ctaaagataa
    25861 cgcgaagggt atgtatgaaa tcattgaaga gcgtcgactt gaagaagcgt aaattggagg
    25921 tgacaagcaa tgaaaaataa agtagaacat atagaaaatc agtacacgtc gcaagagaac
    25981 aagaaaaaac aacgtcaaaa aatgaaaatg cgtgttgttc gtaggcgtat tacagtattt
    26041 gcgggcgtat tacttgcgat aattgttgtt ttatcaatct tgcttgttgt ccaaaaacat
    26101 cgcaatgata ttgatgcaca ggagcgaaaa gcgaaagaag cacagtttca aaagcaacaa
    26161 aatgaagaaa ttgcgttaaa agaaaagttg aataatctga atgacaaaga ttacattgaa
    26221 aaaattgcgc gtgatgatta ttacttaagc aacaaaggtg aagtgatttt taggttgcca
    26281 gaagacaaag attcgtctag ctcaaaatct tcgaaaaaat aaatccaaat tgattcaaaa
    26341 ttatccgagt atagacattg tgaaaaaatc caaacaagga tataataagg gaaaatcgaa
    26401 tcaaatcggg aggatttatt taacatatgt caatcgaagt tggaaataag cttaaaggta
    26461 aagtcactgg tattaaaaag tttggtgcat tcgtagaatt acctgaagga aaaagtggtt
    26521 tagttcacat tagtgaagtc gcagataatt atgttgaaaa cgtagaagag cacctttctg
    26581 ttggtgatga agtagacgta aaagtattat ctattgctga tgatggaaaa attagtcttt
    26641 caattaagaa agctaaagac cgtccacgta gacaacatac gagtaaacca agtcatcaaa
    26701 aaccagtgca aaaagccgaa gattttgaaa agaaattaag caatttctta aaagatagtg
    26761 aagataaatt aacttcaatc aaacgtcaaa cagaatctag acgcggtggc aaaggttcaa
    26821 gacgttaatt aaaataaata aagactgctt cgataaggaa tatatttaga atgatgcgta
    26881 tcgaataatc gattgcagcg ttagacaatc taagactgtt tcttaaataa ggagcagtct
    26941 cttttatttg taatgatata actaagactt ataccatttt tgaaaattgt aaaagtgagg
    27001 tgatgttatg cagttaaata gtaatggttg gcatgttgat gaccatattg ttgtcgctgt
    27061 ttctacaggt attgatagta tgtgtttatt gtatcaacta ctaaatgatt ataaagatag
    27121 ttatagaaaa ctaacatgct tacatgtcaa tcatggcgtt aggtcagctt caattgagga
    27181 agccagattt ttagaagcat actgcgaacg tcatcacatc gatttacata tcaaaaagtt
    27241 agatttgtcg catagtctcg accgaaataa cagcattcag aatgaagctc gaattaaacg
    27301 ttacgaatgg tttgatgaaa tgatgaatgt attagaagcg gatgtattgc taacggcgca
    27361 tcatttggac gatcaattag aaactattat gtatcgtatt tttaatggga aatcaacacg
    27421 taataaacta ggatttgatg agttatcgaa gcgaaaaggt tatcagattt atcgaccact
    27481 tttagctgtc tctaaaaaag aaataaaaca attccaagag agatatcata ttccatattt
    27541 tgaagatgaa tctaataaag ataacaaata tgttagaaat gatattcgta atagaattat
    27601 tccagctatt gatgaaaata atcaacttaa agtatcgcat ttattaaaat taaaacaatg
    27661 gcatgatgaa caatatgata ttttgcaata ttcagctaaa caatttattc aagaatttgt
    27721 gaagtttgat gaacagtcaa aatatttaga ggtttctaga caagctttta ataacttacc
    27781 aaactcatta aagatggttg tgttggactg cctattatca aagtattatg agttgtttaa
    27841 tattagtgct aaaacatacg aagagtggtt taaacaattt agtagtaaga aagcacaatt
    27901 cagtattaat ctcacggata aatggataat tcaaatcgca tatggtaaat taataataat
    27961 ggctaaaaat aatggcgata catattttag agttcaaaca attaaaaagc caggtaatta
    28021 tatttttaac aaatatcgat tagagataca ttctaattta ccaaaatgtt tatttccgct
    28081 tacagtgaga acacgacaaa gtggcgatac atttaaactg aatgggcgcg atggttataa
    28141 gaaagtgaat cgcctgttta tagattgtaa agtgccacag tgggttcggg atcaaatgcc
    28201 aatcgtattg gataaacaac agcgcattat tgcggtagga gatttatatc aacaacaaac
    28261 aataaaaaaa tggattataa ttagtaaaaa tggagatgaa tagcgttatg cataatgatt
    28321 tgaaagaagt attgttaact gaagaagata ttcaaaatat ctgtaaggaa ttgggagcac
    28381 aattaacaaa ggattatcaa ggtaaaccat tagtatgcgt gggtatctta aaaggctcag
    28441 caatgtttat gtcagattta attaaacgaa ttgataccca tttatcaatt gatttcatgg
    28501 atgtttctag ttatcacgga ggcactgagt caactggtga agttcaaatc attaaagatt
    28561 taggttcttc tattgaaaat aaagacgtat taattattga agatatctta gagactggta
    28621 ctacacttaa gtcaattact gaattattac aatctagaaa agttaattca ttagaaatag
    28681 ttactttatt agataaacca aaccgtcgta aagcggacat tgaagctaag tatgtaggta
    28741 aaaaaatacc agatgaattt gttgttggtt acggtttaga ttatcgtgaa ttataccgaa
    28801 acttaccata tatcggtacg ttaaaacctg aagtgtattc aaattaattt tttaatcaat
    28861 ttcagttatt attactatgc gtttgagaaa taatagtgta gactcaaaaa tatgaaaaat
    28921 gtatttcata tatatttaat tttagacaag acatatgtct tgaaaagttg aaaaatatag
    28981 agattgataa aactaatacg ggtgtgaatg acattgatgt taagctcaat tactagctta
    29041 taaaacatgt catatgttac aatttttgtt agttttatta tgggaagtag gaggaaatga
    29101 cgcatgcaga aagcttttcg caatgtgcta gttatcgtaa taataggcgt tattattttt
    29161 ggtctatttt catatttaaa cggtaatgga aatatgccga aacagcttac atataatcaa
    29221 tttactgaga agttggaaaa aggtgacctt aaaactttag aaatccaacc acaacaaaat
    29281 gtctatatgg taagtggtaa aacgaaaaat gatgaagact attcatcaac tattttatat
    29341 aacaacgaaa aagaattaca aaaaattact gatgctgcta aaaagcaaaa cggtgtaaaa
    29401 ttaacgatta aagaagaaga aaaacaaagt gtctttgtga gtatactttc aacattaatt
    29461 ccagttgtag tcatagcgtt attatttatt ttcttcctaa gccaagcaca aggtggcggt
    29521 agtggcggtc gtatgatgaa ctttggtaaa tctaaagcaa aaatgtacga taataataaa
    29581 cgtcgtgttc gtttctctga tgtagcaggg gcagatgaag aaaaacaaga attaattgaa
    29641 attgttgatt tcttgaaaga taataaaaaa ttcaaagaaa tgggatctag gattcctaaa
    29701 ggtgtcttac ttgttggacc tccaggtact ggtaaaacat tacttgctag agcggttgca
    29761 ggtgaagctg gcgcaccatt cttctctatt agtggttcag actttgtaga gatgtttgtt
    29821 ggtgttggtg cgagccgtgt tcgtgactta ttcgataatg ctaagaaaaa cgcgccttgt
    29881 atcatcttta tcgatgagat tgatgctgtt ggtcgtcaac gtggtgcagg tgttggtggc
    29941 ggtcatgatg aacgtgaaca aaccctaaac caattattag ttgaaatgga tggtttcggt
    30001 gaaaatgaag gtatcattat gatagctgct acaaaccgtc ctgatatcct tgacccagcc
    30061 ttattacgtc caggtcgttt tgatagacaa attcaagttg gtcgtccaga tgtgaaaggc
    30121 cgtgaagcaa ttcttcatgt tcatgctaaa aacaaaccac ttgatgaaac ggttgattta
    30181 aaagcaattt cacaacgtac acctggtttc tcaggtgctg atttagagaa cttattaaat
    30241 gaagcatctt taattgctgt acgtgaaggt aaaaagaaaa ttgacatgag agatatcgaa
    30301 gaggcaacgg atagagttat agccggacct gctaagaaat ctcgagttat ttctaagaaa
    30361 gaacgtaata ttgttgctca tcacgaagct ggtcatacaa ttatcggtat ggtacttgat
    30421 gaggcagaag tagtgcataa agttactatt gttccacgtg gacaagcagg tggttatgca
    30481 atgatgctac ctaaacaaga tcgtttctta atgactgaac aagagttatt agataaaatc
    30541 tgtggtttac ttggtggacg tgtatcagaa gatattaact ttaacgaagt atcaacaggt
    30601 gcttcaaatg acttcgaacg tgcaacacaa atcgcacgct caatggttac gcaatatggt
    30661 atgagtaaaa aattaggacc attacagttc ggtcatagca atggtcaagt attcttaggt
    30721 aaagatatgc aaggtgagcc taattattca agccaaatcg catatgaaat tgataaagaa
    30781 gttcaacgaa tcgttaaaga acaatacgaa cgttgtaaac aaattttatt agagcacaaa
    30841 gaacaattaa ttttaattgc tgaaacatta ttaacagaag aaacattagt tgctgaacaa
    30901 attcaatcat tattctacga aggtaaatta cctgaaattg attatgatgc agctaaagtt
    30961 gttaaagatg aagattctga atttaatgat ggtaaattcg gtaaatctta tgaagagatt
    31021 cgtaaagagc aattagaaga tggacaacgt gacgaaagtg aagatcgtaa agaagaaaaa
    31081 gatattgctg aggataaaaa agaagctgat aaatctgatg aaaaagatga accagcacat
    31141 caacaagccc caaatatcga aaaaccttac gatccaaatc acccagacaa taaataatcg
    31201 attatattca gtacctcttt ctatgataaa gttatagaaa gaggtacttt tatcgttttt
    31261 gaaaatacgt attagatttt aagtcgttga attgttatag cagaaaataa ttgtaaaaca
    31321 agttacttca ttatttagaa tgatgggtgt agaataagta caattgttgc attttatgaa
    31381 gtaaagtaat tttttaaata tagagtaata gaggagattg aaataatgac acacgattat
    31441 attgttaaag cattagcatt tgatggagag attagggctt atgctgcttt gacaactgaa
    31501 actgttcaag aagcacaaac gagacattat acatggccga cagcatctgc tgcaatggga
    31561 agaacaatga cagcaacagc tatgatgggc gcaatgttga aaggtgatca aaaattaact
    31621 gtcactgtag atggccaagg acctattgga cgaattattg ccgatgcaaa tgctaaaggc
    31681 gaggtgcgtg cttatgtaga ccatccacaa actcattttc cattaaatga gcaaggtaaa
    31741 cttgatgtaa gacgagcggt agggacaaat ggatctatta tggttgttaa agacgttgga
    31801 atgaaagact atttctctgg agcaagtcca attgtttcag gagaacttgg tgaagatttt
    31861 acttattatt atgctacaag tgaacaaaca ccttcatcgg taggtcttgg tgtattggta
    31921 aatcctgata atacgattaa agcagcagga ggatttatca ttcaagttat gccaggtgcc
    31981 aaagatgaaa caatttcaaa attagaaaaa gcaattagtg aaatgacacc agtttctaaa
    32041 ttaattgaac aaggattaac gccagaagga ttactaaacg aaatcttagg tgaagaccat
    32101 gtgcaaattt tagagaaaat gcctgttcaa tttgaatgta attgtagtca tgagaaattt
    32161 ttaaatgcta ttaaaggatt gggcgaggct gagattcaaa atatgattaa agaagatcat
    32221 ggtgctgaag cagtatgtca tttctgtgga aataaatata aatatactga agaagaatta
    32281 aacgtgttgc tagaaagttt agcgtaattt aatttaaatc aatacgctaa aatgtttatt
    32341 tttagcggtt tagtgaaatg tagaactaaa tagttgtata atccttagtg attttgtttg
    32401 ctttctagaa tttatttgat aaaataattc tatatccgat aaataaacta agatttcaac
    32461 aactaactaa aaaggagtgt tcttaatggc acaaaaacca gtagataata ttactcaaat
    32521 tattggcggt acaccggtag tcaaattgag aaatgtagta gatgacaatg cagcagatgt
    32581 ttatgtaaaa ttggaatatc aaaatccagg tggttctgta aaggatagaa ttgctttagc
    32641 aatgattgaa aaagcagagc gagaaggcaa aattaaacct ggcgatacaa ttgtagaacc
    32701 aacaagtggt aatacaggta tcggtttagc atttgtatgt gctgctaaag gatataaagc
    32761 agtatttact atgcccgaaa caatgagcca agagcgtcgt aatttattaa aagcatacgg
    32821 tgcggaatta gttttaacgc ctggatcaga agcgatgaaa ggtgcaatta aaaaagctaa
    32881 agaattgaaa gaagaacatg gttacttcga gccacaacaa tttgaaaacc ctgcgaaccc
    32941 tgaagttcat gagttaacta caggtcctga gttattacaa caatttgaag ggaaaactat
    33001 cgatgcgttc ctagctggtg ttggtactgg tggtacgtta tctggtgtag gtaaagttct
    33061 gaaaaaagaa tatcctaaca tcgaaattgt tgctatagag cctgaggctt ctccagtatt
    33121 gagcggtggt gagccaggtc cacataaatt acaaggttta ggtgctggat ttattccagg
    33181 cactttgaat acagaaatct atgacagtat tattaaagta ggaaatgata cagcgatgga
    33241 aatgtctcgt cgagttgcta aagaggaagg tattttagca ggtatttcat caggtgctgc
    33301 gatttatgct gccattcaaa aagcaaaaga attaggaaaa ggtaaaacag tagtaacagt
    33361 attgccgagt aatggtgaac gctacttatc aacaccttta tattcattcg atgactaatt
    33421 aatgtcattt aaaagagtga gttatctttt tgagataact tgctcttttt ttctaccatg
    33481 tatattttta aaaatatgag cgttaaatta aacatttttc tgataaaaat atccagtgaa
    33541 tgataagata ataaacgtac atactaataa ctagtaaata gcaggagtaa attttattag
    33601 agttaaacaa tacataatta aagggtggtt aacatgacta aaacaaaaat tatgggcata
    33661 ttaaacgtca cacctgattc attctcagat ggtggaaaat ttaataatgt tgaatcagct
    33721 ataaatagag tgaaagccat gatagatgaa ggtgctgaca ttatagatgt tggaggtgtt
    33781 tcaacgagac ccggtcatga aatggtttca ttagaagaag agatgaacag agtattacct
    33841 gttgttgaag ctattgtcgg ttttgatgta aaaatttcag tcgatacatt tcgaagtgag
    33901 gttgctgaag catgtttaaa attaggcgtt gatatgatta atgatcaatg ggcgggtctg
    33961 tatgatcatc gtatgttcca aattgtagct aaatatgacg cggaaattat tttaatgcat
    34021 aatggaaatg gtaatcgtga tgaaccggtt gtcgaagaaa tgttaacatc tttgttagca
    34081 caagcacatc aagctaaaat agctggtata ccttcaaata aaatttggct agatccaggt
    34141 ataggtttcg ctaaaactag aaatgaagaa gccgaagtta tggcaagact ggatgaactt
    34201 gttgcaacag aatatccagt tttattagcg acaagccgga aacgtttcac taaagagatg
    34261 atgggttatg atacaacacc ggttgaaaga gatgaagtaa ctgcagctac gactgcatat
    34321 ggtattatga aaggcgttag agcagtacgc gttcataatg tcgagttgaa tgctaaatta
    34381 gctaaaggta tagatttttt aaaggagaat gaaaatgcaa gacacaatct ttcttaaagg
    34441 tatgcgcttt tatggatatc atggtgcttt atcagctgaa aatgaaatag ggcaaatttt
    34501 caaagtggat gtaactttga aagtagactt agctgaagct gggcgtactg ataatgttat
    34561 tgatacagtt cattatggtg aagtgttcga agaggttaaa tcaattatgg aaggtaaggc
    34621 cgttaattta cttgagcatc tagctgaacg tattgcaaat cgtataaatt cacaatataa
    34681 tcgtgtaatg gaaacgaaag tgagaatcac taaagaaaac ccaccgattc cgggtcatta
    34741 tgatggagta ggtatcgaaa tagtgaggga gaataaatga ttcaagcata cttaggatta
    34801 ggtagtaata ttggtgatag agaaagccag ttaaacgatg ctataaagat tttgaatgaa
    34861 tatgatggta ttaacgtatc taatatttct ccgatttatg aaacagcacc agttgggtat
    34921 actgagcaac ctaacttttt aaatttgtgt gttgaaattc aaacaacact cacagtatta
    34981 caactgttgg aatgttgttt gaagacagaa gaatgtttac accgtattag aaaggaacga
    35041 tggggtccta gaactttaga tgtggatatt ttgttgtatg gagaagaaat gatagattta
    35101 ccaaaactgt cggtgccaca tccgagaatg aatgaacgtg catttgtttt aatcccatta
    35161 aatgatatag cagcaaatgt cgtagaacca cgttcgaaat tgaaagtgaa agatttagtt
    35221 tttgtcgatg acagtgtaaa gagatataaa taatgtattg ttgagaacat tcatatttat
    35281 tgggaataga ttattgatta ttggagatgt ttccgcattt tgattatctc taaatgcatt
    35341 tgatttcgaa ttagtatatg atgatttagt atgtgttaga attaagttat ttcataaaat
    35401 caaagtaagc aatatttgat aaattgctgt aatttattta ggcttttgtt gaagtttgtg
    35461 gaagataatt tttatacatt tacttagggt tgtattaatg ataatcgtat tttaaatgaa
    35521 taagtaattc ataaacgaaa acagaaattg ctttattaca tttacaagct cttgtaaaat
    35581 taaaaaagtg ataaaattga tgttatgaat ataagaaaac atgccacatc ataactttaa
    35641 ggtgtaatgg ttaatgataa agtattagaa acatcgaaag agatgtatgt tgagcaaaaa
    35701 tgtctgatat tttataaaac tttaaaggaa aatgtttgag tgtaccagtt ggaatactaa
    35761 aggattacaa caagttaaag gagagaaagt tatgtcagaa gaaatgaatg accaaatgtt
    35821 ggttcgacgt caaaaattac aagaattata tgatcttggt atagacccgt ttggttctaa
    35881 atttgaccgt tcaggtttat ctagtgattt gaaagaagag tgggaccagt attctaaaga
    35941 agaattggta gaaaaagaag cggatagtca tgtcgctata gctggacgat taatgactaa
    36001 gcgtggtaaa ggtaaagcag gatttgcaca cgttcaggac ttagctggac aaattcaaat
    36061 ttacgttcgt aaagatcaag ttggcgatga cgaatttgat ttatggaaaa atgctgattt
    36121 aggcgatatc gttggtgttg aaggtgtaat gttcaaaaca aatactggcg aattatcggt
    36181 taaagcgaag aaattcacgc tactaactaa atcattgcga ccattaccgg ataaattcca
    36241 cggtttacag gatattgaac agagatatcg tcaaagatat ttagatttaa ttacgaacga
    36301 agatagcact cgtacattta ttaatcgtag taaaatcatt caagaaatgc gtaattattt
    36361 aaataataaa ggtttcttgg aagtagaaac acctatgatg caccaaattg ctggtggagc
    36421 agctgctaga ccatttgtaa cacatcataa tgcattagat gcaacgttat acatgcgtat
    36481 tgctattgag ttgcatttaa aacgtttaat tgtcggtgga cttgaaaaag tatatgaaat
    36541 tggtagagta ttccgtaatg aaggtgtatc aactagacat aaccctgaat tcacaatgat
    36601 tgaattatat gaagcatatg cagattatca tgacattatg gatttaacag aatctatggt
    36661 gagacatatt gccaatgaag tgttaggttc tgcaaaagta caatacaatg gggaaacgat
    36721 cgatttagaa tctgcttgga ctcgtttgca tattgttgat gctgtaaaag aagctactgg
    36781 tgtagatttt tatgaagtta aaagtgatga agaagctaaa gctttagcta aagaacatgg
    36841 tattgaaatt aaagatacaa tgaaatatgg tcatatttta aatgaattct ttgagcaaaa
    36901 agttgaagaa acacttattc agccaacgtt tatctatggt catccgactg aaatttcacc
    36961 tttagcgaag aaaaatcctg aagatcctag atttactgat cgtttcgaat tgttcattgt
    37021 aggtagagag catgcaaatg catttactga attaaatgat cctattgatc aaaaaggtcg
    37081 ttttgaagcg caacttgttg aaaaagcgca aggtaatgat gaagcgcatg aaatggatga
    37141 agattacatt gaagcgttag aatatggtat gcctccgaca ggtggtcttg gtatcggtat
    37201 tgacagattg gttatgttat taactgactc tccatcaatc agagacgtat tattattccc
    37261 ttatatgaga caaaaataaa tgacgttgat tgttagtaag agctctcgtg tatacaacat
    37321 gtgtatgcga gggctttctt aattatggta attagttcgt gtttgaatgt ttttgatagt
    37381 aatagttaac gatagtggtg ctatttttga ctgttaaaca aggtagttgg ctgatagatg
    37441 aaaatgacga tacgtatata gagtagttcg gatgagaaat gttaacgata gacattgaga
    37501 tatcttataa caaaaattgc tatattagta taatttatct taatcagcta taaaagtact
    37561 ttaaaattgt atagaatgtg tatggttttg tacatatgtg tatgatagaa tactaaaagt
    37621 gttatgaatt agagggcacg agaaatgtca gttttgaaga ataaaaaagt tgattaaaag
    37681 tgttgacttt atcaattgaa tgaagtaata tataaaagtc gccaaaaaca gacgaaacac
    37741 actaaaagct gatgtgacaa agtttacatc aaagtgtaaa attaactatt gcaccttatt
    37801 aattaagcgt gtatcatgaa taagtaagtt attttgtctg gtgactatag caaggaggtc
    37861 acacctgttc ccatgccgaa cacagaagtt aagctcctta gcgtcgatgg tagtcgaact
    37921 tacgttccgc cagagtagaa cgttgccagg caaatgacaa atcggagaat tagctcagct
    37981 g
//
LOCUS       DALAGD010000009        37647 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.009, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000009 DALAGD010000000
VERSION     DALAGD010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 37647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37647
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.009"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            149..1267
                     /gene="ald"
                     /locus_tag="O2701_000358"
     CDS             149..1267
                     /gene="ald"
                     /locus_tag="O2701_000358"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HCZ4292271.1"
                     /translation="MLVAVVKELKQGEGRVACTPENVRKLTDAGHKVIVEKNAGIGSG
                     FSNDMYEKEGAKIVTHEQAWEADLVIKVKEPHESEYQYFKKNQIIWGFLHLASSKEIV
                     EKMQEVGVTAISGETIIKNGKAELLAPMSAIAGQRSAIMGAYYSEAQHGGQGTLVTGV
                     HENVDIPGSTYVIFGGGVAATNAANVALGLNAKVIIIELNDDRIKYLEDMYAEKDVTV
                     VKSTPENLAEQIKKADVFISTILIPGAKPPKLVTREMVKSMKKGSVLIDIAIDQGGTI
                     ETIRPTTISDPVYEEEGVIHYGVPNQPGAVPRTSTMALAQGNIDYILEICDKGLEQAI
                     KDNEALSTGVNIYQGQVTNQGLASSHDLDYKEILNVIE"
     gene            1362..2402
                     /gene="tdcB"
                     /locus_tag="O2701_000359"
     CDS             1362..2402
                     /gene="tdcB"
                     /locus_tag="O2701_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCZ4292272.1"
                     /translation="MTTNTVTLQTAHIVSLGDIEEAKASIKPFIRRTPLIKSMYLSQS
                     ITKGNVFLKLENMQFTGSFKFRGASNKINHLTDEQKEKGIIAASAGNHAQGVALTAKL
                     LGIDATIVMPETAPQAKQQATKGYGAKVILKGKNFNETRLYMEELAKENGMTIVHPYD
                     DKFVMAGQGTIGLEILDDIWNVNTVIVPVGGGGLIAGIATALKSFNPSIHIIGVQSEN
                     VHGMAESFYKRDLTEHRVDSTIADGCDVKVPGEQTYEVVKHLVDEFILVTEEEIEHAM
                     KDLMQRAKIITEGAGALPTAAILSGKINNKWLEDKNVVALVSGGNVDLTRVSGVIEHG
                     LNIADTSKGVVG"
     gene            2433..3755
                     /locus_tag="O2701_000360"
     CDS             2433..3755
                     /locus_tag="O2701_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCZ4292273.1"
                     /translation="MSNGKELQKNIGFFSAFAIVMGTVIGSGVFFKISNVTEVTGTAG
                     MALFVWFLGGIITICAGLTAAELAAAIPETGGLTKYIEYTYGDFWGFLSGWAQSFIYF
                     PANVAALSIVFATQLINLFHLSIGSLIPIAIASALSIVLINFLGSKAGGILQSVTLVI
                     KLIPIIVIVIFGIFQSGDITFSLIPTTGNSGNGFFTAIGSGLLATMFAYDGWIHVGNV
                     AGELKNPKRDLPLAISVGIGCIMAVYLLINATFLLTLPIELLAGNLNAASDTSKILFG
                     ENGGKIITIGILISVYGTINGYTMTGMRVPYAMAERKLLPFSHLFAKLTKSGAPWFGA
                     IIQLIIAIIMMSMGAFDTITNMLIFVIWLFYCMSFVAVIILRKREPNMERPYKVPLYP
                     IIPLIAILAGSFVLINTLFTQFILAIIGILITALGIPVYYYKKKQKAA"
     gene            3912..5303
                     /gene="norB"
                     /locus_tag="O2701_000361"
     CDS             3912..5303
                     /gene="norB"
                     /locus_tag="O2701_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorB"
                     /protein_id="HCZ4292274.1"
                     /translation="MEKPSREAFEGNNKLLIGIVLSVITFWLFAQSLVNVVPILEDSF
                     NTDIGTVNIAVSITALFSGMFVVGAGGLADKYGRIKLTNIGIILNILGSLLIIISNIP
                     LLLIIGRLIQGLSAACIMPATLSIIKSYYIGKDRQRALSYWSIGSWGGSGVCSFFGGA
                     VATLLGWRWIFILSIIISLIALFLIKGTPETKSKSISLNKFDIKGLVLLVIMLLSLNI
                     LITKGSELGVTSLLFITLLAIAIGSFSLFIVLEKRATNPLIDFKLFKNKAYTGATASN
                     FLLNGVAGTLIVANTFVQRGLGYSSLQAGSLSITYLVMVLIMIRVGEKLLQTLGCKKP
                     MLIGTGVLIVGECLISLTFLPEIFYVICCIIGYLFFGLGLGIYATPSTDTAIANAPLE
                     KVGVAAGIYKMASALGGAFGVALSGAVYAIVSNMTNIYTGAMIALWLNAGMGILSFVI
                     ILLLVPKQNDTQL"
     gene            5701..36966
                     /gene="ebh"
                     /locus_tag="O2701_000362"
     CDS             5701..36966
                     /gene="ebh"
                     /locus_tag="O2701_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyperosmolarity resistance protein Ebh"
                     /protein_id="HCZ4292275.1"
                     /translation="MNYRDKIQKFSIRKYTVGTFSTVIATLVFLGFNTSQAHAAETNQ
                     PASVVKQKQQSNNEQTENRESQVQNSQNSQNGQSLSATHENEQPNISQANLVDQKVAQ
                     SSTTNDEQPASQNVNTKKDSATAATTQPDKEQSKHKQNESQSANKNGNDNRAAHVENH
                     EANVVTASDSSDNGNVQHDRNELQAFFDANYHDYRFIDRENADSGTFNYVKGIFDKIN
                     TLLGSNDPINNKDLQLAYKELEQAVALIRTMPQRQQTSRRSNRIQTRSVESRAAEPRS
                     VSDYQNANSSYYVENANDGSGYPVGTYINASSKGAPYNLPTTPWNTLKASDSKEIALM
                     TAKQTGDGYQWVIKFNKGHAPHQNMIFWFALPADQVPVGRTDFVTVNSDGTNVQWSHG
                     AGAGANKPLQQMWEYGVNDPHRSHDFKIRNRSGQVIYDWPTVHIYSLEDLSRASDYFS
                     EAGATPATKAFGRQNFEYINGQKPAESPGVPKVYTFIGQGDASYTISFKTQGPTVNKL
                     YYAAGGRALEYNQLFMYSQLYVESTQDHQQRLNGLRQVVNRTYRIGTTKRVEVSQGNV
                     QTKKVLESTNLNIDDFVDDPLSYVKTPSNKVLGFYSNNANTNAFRPGGAQQLNEYQLS
                     QLFTDQKLQEAARTRNPIRLMIGFDYPDAYGNSETLVPVNLTVLPEIQHNIKFFKNDD
                     TQNIAEKPFSKQAGHPVFYVYAGNQGNASVNLGGSVTSIQPLRINLTSNENFTDKDWQ
                     ITGIPRTLHIENSTNRPNNARERNIELVGNLLPGDYFGTIRFGRKEQLFEIRVKPHTP
                     TITTTAEQLRGTALQKVPVNISGIPLDPSALVYLVAPTNQTTNGGSEADQIPSGYTIL
                     ATGTPDGVHNTITIRPQDYVVFIPPVGKQIRAVVYYNKVVASNMSNAVTILPDDIPPT
                     INNPVGINAKYYRGDEVNFTMGVSDRHSGIKNTTITTLPNGWTSNLTKADKNNGSLSI
                     TGRVSMNQAFNSDITFKVSATDNVNNTTNDSQSKHVSIHVGKISEDAHPIVLGNTEKV
                     VVVNPTAVSNDEKQSIITAFMNKNQNIRGYLASTDPVTVDNNGNVTLHYRDGSSTTLD
                     ATNVMTYEPVVKPEYQTVNAAKTATVTIAKGQSFSIGDIKQYFTLSNGQPIPSGTFTN
                     ITSDRTIPTAQEVSQMNAGTQLYHITATNAYHKDSEDFYISLKIIDVKQPEGDQRVYR
                     TSTYDLTTDEISKVKQAFINANRDVITLAEGDISVTNTPNGANVSTITVNINKGRLTK
                     SFASNLANMNFLRWVNFPQDYTVTWTNAKIANRPTDGGLSWSDDHKSLIYRYDATLGT
                     QITTNDILTMLKATTTVPGLRNNITGNEKSQAEAGGRPNFRTTGYSQSNATTDGQRQF
                     TLNGQVIQVLDIINPSNGYGGQPVTNSNTRANHSNSTVVNVNEPAANGAGAFTIDHVV
                     KSNSTHNASDAVYKAQLYLTPYGPKQYVEHLNQNTGNTTDAINIYFVPSDLVNPTISV
                     GNYTNHQVFSGETFTNTITANDNFGVQSVTVPNTSQITGTVDNNHQHVSATAPNVTSA
                     TNKTINLLATDTSGNTATTSFNVTVKPLRDKYRVGTSSTAANPVRIANISNNATVSQA
                     DQTTIINSLTFTETVPNRSYARASANEITSKTVSNVSRTGNNANVTVTVTYQDGTTST
                     VTVPVKHVIPEIVAHSHYTVQGQDFPAGNGSSASDYFKLSNGSDIADATITWVSGQAP
                     NKDNTRIGEDITVTAHILIDGETTPITKTATYKVVRTVPKHVFETARGVLYPGVSDMY
                     DAKQYVKPVNNSWSTNAQHMNFQFVGTYGPNKDVVGISTRLIRVTYDNRQTEDLTILS
                     KVKPDPPRIDANSVTYKAGLTNQEIKVNNVLNNSSVKLFKADNTPLNVTNITHGSGFS
                     SVVTVSDALPNGGIKAKSSISMNNVTYTTQDEHGQVVTVTRNESVDSNDSATVTVTPQ
                     LQATTEGAVFIKGGDGFDFGHVERFIQNPPHGATVAWHDSPDTWKNTVGNTHKTAVVT
                     LPNGQGTRNVEVPVKVYPVANAKAPSRDVKGQNLTNGTDAMNYITFDPNTNTNGITAA
                     WANRQQPNNQQAGVQHLNVDVTYPGISAAKRVPVTVNVYQFEFPQTTYTTTVGGTLAS
                     GTQASGYAHMQNATGLPTDGFTYKWNRDTTGTNDANWSAMNKPNVAKVVNAKYDVIYN
                     GHTFATSLPAKFVVKDVQPAKPTVTETAAGAITIAPGANQTVNTHAGNVTTYADKLVI
                     KRNGNVVTTFTRRNNTSPWVKEASAATVAGIAGTNNGITVAAGTFNPADTIQVVATQG
                     SGETVSDEQRSDDFTVVAPQPNQATTKIWQNGHIDITPNNPSGHLINPTQAMDIAYTE
                     KVGNGAEHSKTINVVRGQNNQWTIANKPDYVTLDAQTGKVTFNANTIKPNSSITITPK
                     AGTGHSVSSNPSTLTAPAAHTVNTTEIVKDYGSNVTAAEINNAVQVANKRTATIKNGT
                     AMPTNLAGGSTTTIPVTVTYNDGSTEEVQESIFTKADKRELITAKNHLDDPVSTEGKK
                     PGTITQYNNAMHNAQQQINTAKTEAQQVINNERATPQQVSDALTKVRAAQTKIDQAKA
                     LLQNKEDNSQLVTSKNNLQSSVNQVPSTAGMTQQSIDNYNAKKREAETEITAAQRVID
                     NGDATAQQISDEKHRVDNALTALNQAKHDLTADTHALEQAVQQLNRTGTTTGKKPASI
                     TAYNNSIRALQSDLTSAKNSANAIIQKPIRTVQEVQSALTNVNRVNERLTQAINQLVP
                     LADNSALKTAKTKLDEEINKSVTTDGMTQSSIQAYENAKRAGQTESTNAQNVINNGDA
                     TDQQIAAEKTKVEEKYNSLKQAIAGLTPDLAPLQTAKTQLQNDIDQPTSTTGMTSASI
                     AAFNEKLSAARTKIQEIDRVLASHPDVATIRQNVTAANAAKSALDQARNGLTVDKAPL
                     ENAKNQLQHSIDTQTSTTGMTQDSINAYNAKLTAARNKIQQINQVLAGSPTVEQINTN
                     TSTANQAKSDLDHARQALTPDKAPLQTAKTQLEQSINQPTDTTGMTTASLNAYNQKLQ
                     AARQKLTEINQVLNGNPTVQNINDKVTEANQAKDQLNTARQGLTLDRQPALTTLHGAS
                     NLNQAQQNNFTQQINAAQNHAALETIKSNITALNTAMTKLKDSVADNNTIKSDQNYTD
                     ATPANKQAYDNAVNAAKGVIGETTNPTMDVNTVNQKAASVKSTKDALDGQQNLQRAKT
                     EATNAITHASDLNQAQKNALTQQVNSAQNVQAVNDIKQTTQSLNTAMTGLKRGVANHN
                     QVVQSDNYVNADTNKKNDYNNAYNHANDIINGNAQHPVITPSDVNNALSNVTSKEHAL
                     NGEAKLNAAKQEANTALGHLNNLNNAQRQNLQSQINGAHQIDAVNTIKQNATNLNSAM
                     GNLRQAVADKDQVKRTEDYADADTAKQNAYNSAVSSAETIINQTTNPTMSVDDVNRAT
                     SAVTSNKNALNGYEKLAQSKTDAARAIDALPHLNNAQKADVKSKINAASNIAGVNTVK
                     QQGTDLNTAMGNLQGAINDEQTTLNSQNYQDATPSKKTAYTNAVQAAKDILNKSNGQN
                     KTKDQVTEAMNQVNSAKNNLDGTRLLDQAKQTAKQQLNNMTHLTTAQKTNLTNQINSG
                     TTVAGVQTVQSNANTLDQAMNTLRQSIANKDATKASEDYVDANNDKQTAYNNAVAAAE
                     TIINANSNPEMNPSTITQKAEQVNSSKTALNGDENLAAAKQNAKTYLNTLTSITDAQK
                     NNLISQITSATRVSGVDTVKQNAQHLDQAMASLQNGINNESQVKSSEKYRDADTNKQQ
                     EYDNAITAAKAILNKSTGPNTAQNAVEAALQRVNNAKDALNGDAKLIAAQNAAKQHLG
                     TLTHITTAQRNDLTNQISQATNLAGVESVKQNANSLDGAMGNLQTAINDKSGTLASQN
                     FLDADEQKRNAYNQAVSAAETILNKQTGPNTAKTAVEQALNNVNNAKHALNGTQNLNN
                     AKQAAITAINGASDLNQKQKDALKAQANGAQRVSNAQDVQHNATELNTAMGTLKHAIA
                     DKTNTLASSKYVNADSTKQNAYTTKVTNAEHIISGTPTVVTTPSEVTAAANQVNSAKQ
                     ELNGDERLREAKQNANTAIDALTQLNTPQKAKLKEQVGQANRLEDVQTVQTNGQALNN
                     AMKGLRDSIANETTVKTSQNYTDASPNNQSTYNSAVSNAKGIINQTNNPTMDTSAITQ
                     ATTQVNNAKNGLNGAENLRNAQNTAKQNLNTLSHLTNNQKSAISSQIDRAGHVSEVTA
                     TKNAATELNTQMGNLEQAIHDQNTVKQSVKFTDADKAKRDAYTNAVSRAEAILNKTQG
                     ANTSKQDVEAAIQNVSSAKNALNGDQNVTNAKNAAKNALNNLTSINNAQKRDLTTKID
                     QATTVAGVEAVSNTSTQLNTAMANLQNGINDKTNTLASENYHDADSDKKTAYTQAVTN
                     AENILNKNSGSNLDKTAVENALSQVANAKGALNGNHNLEQAKSNANTTINGLQHLTTA
                     QKDKLKQQVQQAQNVAGVDTVKSSANTLNGAMGTLRNSIQDNTATKNGQNYLDATERN
                     KTNYNNAVDSANGVINATSNPNMDANAINQIATQVTSTKNALDGTHNLTQAKQTATNA
                     IDGATNLNKAQKDALKAQVTSAQRVANVTSIQQTANELNTAMGQLQHGIDDENATKQT
                     QKYRDAEQSKKTAYDQAVAAAKAILNKQTGSNSDKAAVDRALQQVTSTKDALNGDAKL
                     AEAKAAAKQNLGTLNHITNAQRTDLEGQINQATTVDGVNTVKTNANTLDGAMNSLQGS
                     INDKDATLRNQNYLDADESKRNAYTQAVTAAEGILNKQTGGNTSKADVDNALNAVTRA
                     KAALNGADNLRNAKTSATNTIDGLPNLTQLQKDNLKHQVEQAQNVAGVNGVKDKGNTL
                     NTAMGALRTSIQNDNTTKTSQNYLDASDSNKNNYNTAVNNANGVINATNNPNMDANAI
                     NGMANQVNTTKAALNGAQNLAQAKTNATNTINNAHDLNQKQKDALKTQVNNAQRVSDA
                     NNVQHTATELNSAMTALKAAIADKERTKASGNYVNADQEKRQAYDSKVTNAENIISGT
                     PNATLTVNDVNSAASQVNAAKTALNGDNNLRVAKEHANNTIDGLAQLNNAQKAKLKEQ
                     VQSATTLDGVQTVKNSSQTLNTAMKGLRDSIANEATIKAGQNYTDASPNNRNEYDSAV
                     TAAKAIINQTSNPTMEPNTITQVTSQVTTKEQALNGARNLAQAKTTAKNNLNNLTSIN
                     NAQKDALTRSIDGATTVAGVNQETAKATELNNAMHSLQNGINDETQTKQTQKYLDAEP
                     SKKSAYDQAVNAAKAILTKASGQNVDKAAVEQALQNVNSTKTALNGDAKLNEAKAAAK
                     QTLGTLTHINNAQRTALDNEITQATNVEGVNTVKAKAQQLDGAMGQLETSIRDKDTTL
                     QSQNYQDADDAKRTAYSQAVNAAATILNKTAGGNTPKADVERAMQAVTQANTALNGIQ
                     NLDRAKQAANTAITNASDLNTKQKEALKAQVTSAGRVSAANGVEHTATELNTAMTALK
                     RAIADKAETKASGNYVNADANKRQAYDEKVTAAENIVSGTPTPTLTPADVTNAATQVT
                     NAKTQLNGNHNLEVAKQNANTAIDGLTSLNGPQKAKLKEQVGQATTLPNVQTVRDNAQ
                     TLNTAMKGLRDSIANEATIKAGQNYTDASQNKQTDYNSAVTAAKAIIGQTTSPSMNAQ
                     EINQAKDQVTAKQQALNGQENLRTAQTNAKQHLNGLSDLTDAQKDAVKRQIEGATHVN
                     EVTQAQNNADALNTAMTNLKNGIQDQNTIKQGVNFTDADEAKRNAYTNAVTQAEQILN
                     KAQGPNTSKDGVETALENVQRAKNELNGNQNVANAKTTAKNALNNLTSINNAQKEALK
                     SQIEGATTVAGVNQVSTTASELNTAMSNLQNGINDEAATKAAQKYTDADREKQTAYND
                     AVTAAKTLLDKTAGSNDNKAAVEQALQRVNTAKTALNGDERLNEAKNTAKQQVATMSH
                     LTDAQKANLTSQIESGTTVAGVQGIQANAGTLDQAMNQLRQSIASKDATKSSEDYQDA
                     NADLQNAYNDAVTNAEGIISATNNPEMNPDTINQKASQVNSAKSALNGDEKLAAAKQT
                     AKSDIGRLTDLNNAQRTAANAEVDQAPNLAAVTAAKNKATSLNTAMGNLKHALAEKDN
                     TKRSVNYTDADQPKQQAYDTAVTQAEAITNANGSNANETQVQAALNQLNQAKNDLNGD
                     NKVAQAKESAKRALASYSNLNNAQSTAAISQIDNATTVAGVTAAQNTANELNTAMGQL
                     QNGINDQNTVKQQVNFTDADQGKKDAYTNAVTNAQGILDKAHGQNMTKAQVEAALNQV
                     TTAKNALNGDANVRQAKSDAKANLGTLTHLNNAQKQDLTSQIEGATTVNGVNGVKTKA
                     QDLDGAMQRLQSAIANKDQTKASENYIDADPTKKTAFDNAITQAESYLNKDHGANKDK
                     QAVEQAIQSVTSTENALNGDANLQRAKTEAIQAIDNLTHLNTPQKTALKQQVNAAQRV
                     SGVTDLKNSATSLNNAMDQLKQAIADHDTIVASGNYTNASPDKQGAYTDAYNAAKNIV
                     NGSPNVITNAADVTAATQRVNNAETGLNGDTNLATAKQQAKDALRQMTHLSDAQKQSI
                     TGQIDSATQVTGVQSVKDNATNLDNAMNQLRNSIANKDDVKASQPYVDADRDKQNAYN
                     TAVTNAENIINATSQPTLDPSAVTQAANQVSTNKTALNGAQNLANKKQETTANINQLS
                     HLNNAQKQDLNTQVTNAPNISTVNQVKTKAEQLDQAMERLINGIQDKDQVKQSVNFTD
                     ADPEKQTAYNNAVTAAENIINQANGTNANQSQVEAALSTVTTTKQALNGDRKVTDAKN
                     NANQTLSTLDNLNNAQKGAVTGNINQAHTVAEVTQAIQTAQELNTAMGNLKNSLNDKD
                     TTLGSQNFADADPEKKNAYNEAVHNAENILNKSTGTNVPKDQVEAAMNQVNATKAALN
                     GTQNLEKAKQHANTAIDGLSHLTNAQKEALKQLVQQSTTVAEAQGNEQKANNVDAAMD
                     KLRQSIADNATTKQNQNYTDASQNKKDAYNNAVTTAQGIIDQTTSPTLDPTVINQAAG
                     QVSTTKNALNGNENLEAAKQQASQSLGSLDNLNNAQKQTVTDQINGAHTVDEANQIKQ
                     NAQNLNTAMGNLKQAIADKDATKATVNFTDADQAKQQAYNTAVTNAENIISKANGGNA
                     TQAEVEQAIKQVNAAKQALNGNANVQHAKDEATALINSSNDLNQAQKDALKQQVQNAT
                     TVAGVNNVKQTAQELNNAMTQLKQGIADKEQTKADGNFVNADPDKQNAYNQAVAKAEA
                     LISATPDVVVTPSEITAALNKVTQAKNDLNGNTNLATAKQNVQHAIDQLPNLNQAQRD
                     EYSKQITQATLVPNVNAIQQAATTLNDAMTQLKQGIANKAQIKGSENYHDADTDKQTA
                     YDNAVTKAEELLKQTTNPTMDPNTIQQALTKVNDTNQALNGNQKLADAKQDAKTTLGT
                     LDHLNDAQKQALTTQVEQAPDIATVNNVKQNAQNLNNAMTNLNNALQDKTETLNSINF
                     TDADQAKKDAYTNAVSHAEGILSKANGSNASQTEVEQAMQRVNEAKQALNGNDNVQRA
                     KDAAKQVITNANDLNQAQKDALKQQVDAAQTVANVNTIKQTAQDLNQAMTQLKQGIAD
                     KDQTKANGNFVNADTDKQNAYNNAVAHAEQIISGTPNANVDPQQVAQALQQVNQAKGD
                     LNGNHNLQVAKDNANTAIDQLPNLNQPQKTALKDQVSHAELVTGVNAIKQNADALNNA
                     MGTLKQQIQANSQVPQSVDFTQADQDKQQAYNNAANQAQQIANGIPTPVLTPDTVTQA
                     VTTMNQAKDALNGDEKLAQAKQEALANLDTLRDLNQPQRDALRNQINQAQALATVEQT
                     KQNAQNVNTAMSNLKQGIANKDTVKASENYHDADADKQTAYTNAVSQAEGIINQTTNP
                     TLNPDEITRALTQVTDAKNGLNGEAKLATEKQNAKDAVSGMTHLNDAQKQALKGQIDQ
                     SPEIATVNQVKQTATSLDQAMDQLSQAINDKAQTLADGNYLNADPDKQNAYKQAVAKA
                     EALLNKQSGTNEVQAQVESITNEVNAAKQALNGNDNLANAKQQAKQQLANLTHLNDAQ
                     KQSFESQITQAPLVTDVTTINQKAQTLDHAMELLRNSVADNQTTLASEDYHDATAQRQ
                     NDYNQAVTAANNIINQTTSPTMNPDDVNGATTQVNNTKVALDGDENLAAAKQQANNRL
                     DQLDHLNNAQKQQLQSQITQSSDIAAVNGHKQTAESLNTAMGNLINAIADHQAVEQRG
                     NFINADTDKQTAYNTAVNEAAAMINKQTGQNANQTEVEQAITKVQTTLQALNGDHNLQ
                     VAKTNATQAIDALTSLNDPQKTALKDQVTAATLVTAVHQIEQNANTLNQAMHGLRQSI
                     QDNAATKANSKYINEDQPEQQNYDQAVQAANNIINEQTATLDNNAINQAATTVNTTKA
                     ALHGDVKLQNDKDHAKQTVSQLAHLNNAQKHMEDTLIDSETTRTAVKQDLTEAQALDQ
                     LMDALQQSIADKDATRASSAYVNAEPNKKQSYDEAVQNAESIIAGLNNPTINKGNVSS
                     ATQAVISSKNALDGVERLAQDKQTAGNSLNHLDQLTPAQQQALENQINNATTRGEVAQ
                     KLTEAQALNQAMEALRNSIQDQQQTEAGSKFINEDKPQKDAYQAAVQNAKDLINQTNN
                     PTLDKAQVEQLTQAVNQAKDNLHGDQKLADDKQHAVTDLNQLNGLNNPQRQALESQIN
                     NAATRGEVAQKLAEAKALDQAMQALRNSIQDQQQTESGSKFINEDKPQKDAYQAAVQN
                     AKDLINQTGNPTLDKSQVEQLTQAVTTAKDNLHGDQKLARDQQQAVTTVNALPNLNHA
                     QQQALTDAINAAPTRTEVAQHVQTATELDHAMETLKNKVDQVNTDKAQPNYTEASTDK
                     KEAVDQALQAAESITDPTNGSNANKDAVDQVLTKLQEKENELNGNERVAEAKTQAKQT
                     IDQLTHLNADQIATAKQNIDQATKLQPIAELVDQATQLNQSMDQLQQAVNEHANVEQT
                     VDYTQADSDKQNAYKQAIADAENVLKQNANKQQVDQALQNILNAKQALNGDERVALAK
                     TNGKHDIDQLNALNNAQQDGFKGRIDQSNDLNQIQQIVDEAKALNRAMDQLSQEITDN
                     EGRTKGSTNYVNADTQVKQVYDETVDKAKQALDKSTGQNLTAKQVIKLNDAVTAAKKA
                     LNGEERLNNRKAEALQRLDQLTHLNNAQRQLAIQQINNAETLNKASRAINRATKLDNA
                     MGAVQQYIDEQHLGVISSTNYINADDNLKANYDNAIANAAHELDKVQGNAIAKAEAEQ
                     LKQNIIDAQNALNGDQNLANAKDKANAFVNSLNGLNQQQQDLAHKAINNADTVSDVTD
                     IVNNQIDLNDAMETLKHLVDNEIPNAEQTVNYQNADDNAKTNFDDAKRLANTLLNSDN
                     TNVNDINGAIQAVNDAIHNLNGDQRLQDAKDKAIQSINQALANKLKEIEASNATDQDK
                     LIAKNKAEELANSIINNINKATSNQAVSQVQTAGNHAIEQVHANEIPKAKIDANKDVD
                     KQVQALIDEIDRNPNLTDKEKQALKDRINQILQQGHNGINNAMTKEEIEQAKAQLAQA
                     LQDIKDLVKAKEDAKQDVDKQVQALIDEIDQNPNLTDKEKQALKYRINQILQQGHNDI
                     NNALTKEEIEQAKAQLAQALQDIKDLVKAKEDAKNAIKALANAKRDQINSNPDLTPEQ
                     KAKALKEIDEAEKRALQNVENAQTIDQLNRGLNLGLDDIRNTHVWEVDEQPAVNEIFE
                     ATPEQILVNGELIVHRDDIITEQDILAHINLIDQLSAEVIDTPSTATISDSLTAKVEV
                     TLLDGSKVIVNVPVKVVEKELSVVKQQAIESIENAAQQKINEINNSVTLTLEQKEAAI
                     AEVNKLKQQAIDHVNNAPDVHSVEEIQQQEQAHIEQFNPEQFTIEQAKSNAIKSIEDA
                     IQHMIDEIKARTDLTDKEKQEAIAKLNQLKEQAIQAIQRAQSIDEISEQLEQFKAQMK
                     AANPTAKELAKRKQEAISRIKDFSNEKINSIRNSEIGTADEKQAAMNQINEIVLETIR
                     DINNAHTLQQVEAALNNGIARISAVQIVTSDRAKQSSSTGNESNSHLTIGYGTANHPF
                     NSSTIGHKKKLDEDDDIDPLHMRHFSNNFGNVIKNAIGVVGISGLLASFWFFIAKRRR
                     KEDEEEELEIRDNNKDSIKETLDDTKHLPLLFAKRRRKEDEEDVTVEEKDSLNNGESL
                     DKVKHTPFFLPKRRRKEDEEDVEVTNENTDEKVLKDNEHSPLLFAKRRKDKEEDVETT
                     TSIESKDEDVPLLLAKKKNQKDNQSKDKKSASKNTSKKVAAKKKKKKAKKNKK"
     gene            complement(37025..37426)
                     /locus_tag="O2701_000363"
     CDS             complement(37025..37426)
                     /locus_tag="O2701_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HCZ4292276.1"
                     /translation="MAKINFDAATKGNPGISTCAIVIKEDEQHYTYTHELGEMDNHTA
                     EWAACIYALEHARELNVQNALLYTDSKLIADSIEAGYVKNANFKPYFDQIEIFEKDFD
                     LLFVKWIPREQNKEANQHAQQALYKLIKKNK"
BASE COUNT        14940 a         6555 c         6806 g         9346 t
ORIGIN      
        1 taatatttaa gaataagaat aatatattaa atatttatta acaaattata gataaaatat
       61 gaataattaa ttaatggtat ttacatattc ataaccaaag gataaaataa ttgtgaaatt
      121 attcacaaat gaaagaggag tgttgcaaat gttagttgca gtagtcaaag aattaaaaca
      181 aggtgaagga cgtgtagctt gcacacccga aaatgtgcgt aagttaacgg atgcaggaca
      241 taaagtaatt gttgaaaaaa atgctggcat tggttcagga ttttctaacg atatgtatga
      301 aaaagaaggc gctaagatcg taactcacga acaagcatgg gaagctgatc ttgttatcaa
      361 agtaaaagaa cctcatgaaa gcgaatatca atatttcaaa aagaatcaaa ttatctgggg
      421 atttttacat ctagcatctt caaaagaaat agtagaaaaa atgcaagaag ttggtgtaac
      481 tgcgattagt ggtgaaacca ttataaaaaa tggaaaagca gaattattag cgccaatgag
      541 tgctatagca ggtcaacgct cagcaattat gggagcttac tactctgaag cacaacatgg
      601 tggtcaaggt actttagtga ctggtgtaca tgaaaatgtg gatatacctg gtagtacata
      661 tgtgattttc ggtggtggag tagcagcaac aaatgcagca aatgttgcct tgggactaaa
      721 tgctaaagta atcattatcg agttaaacga tgaccgcatt aaatatcttg aagatatgta
      781 tgcagaaaaa gatgtcacag tagtcaaatc aacaccagaa aatttagcag aacaaattaa
      841 gaaagcagat gtatttattt ctacaatttt aattccaggt gcgaaaccgc caaaattggt
      901 tactcgtgag atggttaaat caatgaaaaa aggttcagta ttaatcgata tagctattga
      961 ccaaggtgga actattgaaa caattagacc aactacaatt tctgatccag tgtatgaaga
     1021 agaaggtgtg attcattatg gtgtaccaaa tcaaccagga gcagtcccaa gaacttcaac
     1081 aatggcatta gcacaaggaa atattgatta tatattagaa atttgtgaca aaggcttaga
     1141 acaagcaatt aaagataatg aagccttaag tactggtgta aacatttacc aaggacaagt
     1201 gacaaatcaa ggattagctt catcacatga cctagattat aaagaaatat taaatgttat
     1261 cgaatagata gtaatttaaa tgaaattgag tgaaatgaat attttaaata tagcattata
     1321 gtttggacta aaaatttaca aaacggaagg atgtaattat tatgacaacc aacacagtta
     1381 cattacaaac agcacatatt gtaagtttag gagatatcga agaagctaaa gcaagcatta
     1441 aaccatttat tcgtcgaaca cctctaatta aatcaatgta tttaagccaa agtataacta
     1501 aagggaatgt atttctaaaa ttagaaaata tgcaattcac aggatctttt aaatttagag
     1561 gcgctagcaa taaaattaat cacttaacag atgaacaaaa agaaaaaggc attatcgcag
     1621 catctgctgg gaaccatgca caaggtgttg ctttaacagc taaattatta ggcattgatg
     1681 caacgattgt aatgcctgaa acagcaccac aagcgaaaca acaagcaaca aaaggctatg
     1741 gggcaaaggt tattttaaaa ggtaaaaact ttaacgaaac tagactttat atggaagaat
     1801 tagcgaaaga aaatggcatg acaatcgttc atccatatga cgataagttt gtaatggcag
     1861 gccaaggaac aattggttta gaaattttag atgatatttg gaatgtgaat acagtcatcg
     1921 taccagttgg cggtggagga ttaattgcag gtattgccac cgcattaaaa tcatttaacc
     1981 cttcaattca tattatcggt gttcaatctg agaatgttca tggtatggct gagtctttct
     2041 ataagagaga tttaactgaa catcgagtgg atagcacaat agcagatggt tgtgatgtaa
     2101 aagttcctgg tgaacaaaca tatgaagtag ttaaacattt agtagatgaa tttattcttg
     2161 ttactgaaga agaaattgaa catgctatga aagatttaat gcagcgtgcc aaaattatta
     2221 ctgaaggtgc aggcgcatta ccaacagctg caattttaag tggaaaaata aacaataaat
     2281 ggcttgaaga taaaaatgtt gttgcattag tttcaggcgg gaatgttgac ttaactagag
     2341 tttcaggtgt cattgaacat ggactgaata ttgcagatac aagcaagggt gtggtaggtt
     2401 aaaacattta atcttaaaaa tgaggtgtaa ttatgtcaaa tggtaaagaa ttacaaaaaa
     2461 atataggttt cttctcagcg tttgctattg ttatggggac agttattggt tcaggagtat
     2521 tctttaaaat atcaaacgta acagaagtaa caggaacagc aggaatggcc ttgtttgtat
     2581 ggttcctagg cggcatcatt accatttgtg cggggttaac agcagcagaa cttgctgctg
     2641 caatccctga aacaggtggc ttaacgaagt atatagaata tacatacggt gatttctggg
     2701 gcttcctatc aggttgggcg caatcattta tttattttcc agctaacgta gcagcattgt
     2761 ctatcgtatt tgcgacacag ctaattaatt tattccattt atctataggt tcgttaatac
     2821 caatagcaat cgcatctgcg ttatctattg tgttgataaa tttcctaggt tcaaaagcag
     2881 gcggaatttt acaatcagtt actttagtaa ttaaactgat tccaatcatc gttattgtaa
     2941 tttttggtat ttttcaatct ggagatatca ctttttcatt aattccaact acaggtaatt
     3001 caggaaatgg cttctttaca gcaattggta gtggtttatt agcaactatg tttgcatatg
     3061 atggttggat tcatgtagga aatgttgcgg gggaacttaa aaatcctaaa cgcgatttac
     3121 ctttagcgat ttcagttggt atcggttgta ttatggctgt gtatttatta attaacgcta
     3181 cattcttatt aacgttgcca atagaactac ttgctggtaa tttaaatgca gcttcagata
     3241 catcaaaaat attatttggt gaaaatggcg gtaagattat tacaatcggt atattaattt
     3301 ctgtttatgg tacgatcaat ggctatacta tgactggtat gcgcgtacca tatgcaatgg
     3361 ctgaaagaaa attattgcca tttagccatt tattcgcaaa attaacaaaa tctggcgcac
     3421 catggtttgg cgcaattata caacttataa tcgctatcat catgatgtca atgggagcat
     3481 ttgatacaat tacaaatatg ttaatctttg ttatttggtt gttctattgt atgtcatttg
     3541 ttgcggtaat aattttaaga aaacgtgaac caaatatgga acgaccatat aaagtaccgt
     3601 tatatccgat cataccttta attgctattt tggcaggatc atttgtatta attaatacac
     3661 tgtttacaca atttatatta gcaatcattg gaattctaat aacagcactt ggtataccag
     3721 tttattacta taaaaagaaa caaaaagcag cataaggtaa gataactagc attgagaata
     3781 aatggatgga ctactaataa atttaaagtt ttacacatta aaatcaaaaa ccattcaatt
     3841 attctatgga acagacaaat ttctgttatg gaatttgtct gtttttcaaa agtataggga
     3901 ggcaaataga gatggaaaag ccgtcaagag aggcatttga aggcaataat aagttgttaa
     3961 taggaattgt tctaagtgta ataacgtttt ggctatttgc acaatcattg gttaatgttg
     4021 taccaatact tgaagatagt ttcaatacag atattggaac ggttaatatc gccgttagta
     4081 taactgcttt attttcagga atgtttgtag taggagcagg tggtcttgct gataaatatg
     4141 gcagaattaa actcacgaac attggtatta tcttaaatat attaggttca ttattaatca
     4201 ttatttcaaa tattccttta ttacttatta taggaagatt aattcaagga ctttcagcag
     4261 catgtattat gcctgcaact ttgtctatta ttaagtcata ttacattggg aaagatagac
     4321 aacgcgcttt aagttattgg tcaattggct catggggcgg ctctggtgtt tgttcatttt
     4381 ttggaggtgc agttgcaacg cttttaggtt ggcgttggat tttcatccta tcaattataa
     4441 tttcattaat tgcactgttt cttattaaag gcacacctga aactaaatct aaatcgattt
     4501 ctctaaataa atttgacatt aaaggtctgg ttcttttagt cattatgctc ctcagtttaa
     4561 atattttaat tactaaagga tcagaattag gtgtaacctc acttcttttt attactttat
     4621 tagctattgc aattggatct tttagtttat ttatagttct tgaaaagcgt gctacaaatc
     4681 ctttaatcga ttttaaatta tttaaaaata aagcttacac aggtgcaaca gcttcaaact
     4741 ttttgttaaa tggtgttgca ggaacattaa tagtagccaa cacatttgtt caaagaggtt
     4801 taggatattc ttcattgcaa gcaggaagtt tatcaatcac ttatttagta atggtactaa
     4861 ttatgattcg tgttggtgaa aagttacttc aaacactcgg atgcaagaaa ccaatgttaa
     4921 ttggaacagg agttcttatt gtcggagaat gtctcatttc attaactttc ttgccagaaa
     4981 tattctatgt catttgttgt attataggtt atttattctt tggtttagga ctagggatat
     5041 atgctacacc atcaacagat acagcaattg caaatgcacc gttagaaaaa gtaggcgttg
     5101 ctgcaggtat ctataaaatg gcttctgcat taggtggagc atttggcgtc gcattgagtg
     5161 gtgcagtata tgcaatcgta tcaaatatga caaacattta tacaggtgca atgattgcat
     5221 tatggttaaa tgcaggtatg ggaatattat cattcgttat cattttgtta cttgtgccta
     5281 aacaaaacga tactcaatta tgataattga gaattaaatt gaaatcatac aagtcgctac
     5341 aatattaaac aaaaatataa accgattctt atgtgtcatt attttaaatg aacataggga
     5401 ttggtttttt attactcttt tacgctactt tatttataat tattataaat tgtcacaaat
     5461 tcaatttacc ttacaatata ttttgtgtta ttatattctg gagcataaat aaattgttca
     5521 acacatagtt gtaatgtgtt tcaatacttt ttggatagat tgcgaaattg tattgaatcg
     5581 tcatcgtttt aaatttttaa atgagaatgg aatgagcatt acaatacaca agcaatcaaa
     5641 agtaaataca ttcacaacac aacagagaca taacaacaag ataaggagtg aacaatagct
     5701 gtgaattatc gtgataaaat tcaaaagttt agtattcgta aatatacagt tggtacattt
     5761 tcaactgtca ttgcgacatt ggtattttta ggattcaata catcacaagc acatgctgct
     5821 gaaacaaatc aaccagcaag cgtggttaaa cagaaacaac aaagtaataa tgaacagact
     5881 gagaatcgag aatctcaagt acaaaattct caaaattcac aaaatggtca atcattatct
     5941 gctactcatg aaaatgagca accaaatatt agtcaagcta atttagtaga tcaaaaagta
     6001 gcgcaatcat ctactactaa tgatgaacaa ccagcatctc aaaatgtaaa tacaaagaaa
     6061 gattcggcaa cggctgcgac aacacaacca gataaagaac aaagtaagca taaacaaaac
     6121 gaaagtcaat ctgctaataa aaatggaaac gacaatagag cggctcatgt agaaaatcat
     6181 gaagcaaatg tagtaacagc ttcagattca tctgataatg gtaacgtaca acatgaccga
     6241 aatgaattac aagcgttttt tgatgcaaat tatcatgatt atcgctttat tgaccgtgaa
     6301 aatgcagatt ctggcacatt taactatgta aaaggcattt ttgataagat taatacgtta
     6361 ttaggcagta atgatccaat aaacaataaa gacttgcaac ttgcatacaa agaattggaa
     6421 caagctgttg ctttaattcg tacaatgcct caacgtcaac agactagccg acgttcaaat
     6481 agaattcaaa cgcgttcggt tgagtcaaga gctgcagagc ctagatcagt atcagactat
     6541 caaaatgcaa attcatcata ttatgttgaa aatgctaatg atggttcggg ctatcctgtt
     6601 ggtacatata tcaatgcttc tagtaaaggg gcgccatata atttaccaac tacaccatgg
     6661 aatacattga aggcctctga ctcaaaggaa attgctctta tgacagcgaa acaaactgga
     6721 gacgggtacc aatgggttat taagtttaat aaaggacatg ctccacatca aaatatgatc
     6781 ttttggtttg cattaccagc agaccaagtg ccagtaggaa gaactgactt tgtaacagtt
     6841 aattcagatg gaacaaatgt acaatggagt catggagcag gagcaggtgc aaataaacca
     6901 cttcaacaaa tgtgggaata tggagtaaat gatcctcatc gttcacatga ctttaaaata
     6961 agaaatagaa gtggccaagt aatatatgac tggccaactg tccatattta ttctttagaa
     7021 gatttatcta gagcgagtga ttattttagt gaagctggag cgacacctgc tactaaagct
     7081 tttggtagac aaaattttga atatattaat ggtcaaaaac ctgctgaatc accgggtgtt
     7141 cctaaagttt atactttcat cggtcaaggt gatgcaagtt atacaatttc atttaaaaca
     7201 caaggtccaa ctgttaataa attgtactat gcagcaggtg ggcgtgcttt agagtacaat
     7261 caattattta tgtacagtca actatacgtc gaatcaacgc aagaccatca acaacgtctt
     7321 aatggtttaa gacaagtggt taatcgtaca tatcgcatag gtacaactaa acgtgtagaa
     7381 gtgagtcaag gaaatgtaca aacgaaaaag gtattagaaa gtacaaacct aaatatagat
     7441 gattttgttg atgatccttt aagttatgtt aagacgccga gtaataaagt gttaggattt
     7501 tattcgaata atgcaaatac taatgctttt agaccgggtg gagcccaaca attaaatgaa
     7561 tatcaattaa gtcaattatt tactgatcaa aaattacaag aagcagcaag aactagaaac
     7621 ccaataagat taatgattgg tttcgactat cctgatgctt atggtaatag tgaaacttta
     7681 gttcctgtta acttaacggt attacctgaa atccaacata atattaaatt ctttaaaaat
     7741 gacgatactc aaaatattgc tgaaaaacca ttttcaaaac aagctgggca tccagttttc
     7801 tatgtatatg caggtaacca agggaatgct tccgtgaatt taggtggtag cgtaacatct
     7861 attcaaccat tacgtattaa tttaacaagt aatgagaatt ttacagataa agattggcaa
     7921 attacaggta ttccgcgtac attacacatt gaaaactcga caaatagacc taataatgcc
     7981 agagaacgca atattgaact tgttggtaac ttattaccag gggattactt tggaacgata
     8041 cgttttggac gtaaagaaca attattcgaa attcgtgtta aaccacatac accaacaatt
     8101 acaacgacag ctgagcaatt aagaggtaca gcattacaaa aagtgcctgt taatatttcg
     8161 ggaataccgt tggatccatc ggcattggtt tatttagttg caccaacaaa tcaaactacg
     8221 aatggtggta gtgaggcaga tcaaatacca tctggttata cgatacttgc gactggtaca
     8281 cctgatgggg tgcataatac aattactata cgaccgcaag attatgttgt attcatacca
     8341 cctgtaggta aacaaattag agcagtagtt tattataata aagtagttgc atctaatatg
     8401 agtaatgctg ttactatttt gccagatgac attccaccaa caatcaataa tcctgttgga
     8461 ataaatgcca aatactatcg aggcgacgaa gtcaacttta caatgggtgt ctctgataga
     8521 cattctggta taaaaaatac aactattacg acattgccga atggttggac atcaaattta
     8581 acaaaagcgg ataagaataa tggctcatta tctattacag gtagagtgag tatgaatcag
     8641 gcatttaaca gtgatattac atttaaagtg tcagcgacag acaatgtcaa taatacgaca
     8701 aatgatagtc aatctaaaca tgtttcaatt catgtaggta aaattagtga agatgctcat
     8761 ccgattgtat taggaaatac tgagaaagtt gtagtagtca atccgactgc tgtatctaat
     8821 gatgaaaagc aaagcataat tactgccttt atgaataaaa accaaaatat aagaggatat
     8881 ttagcatcaa ctgatccagt aactgtcgat aataatggta atgtcacatt acattaccgt
     8941 gatggctcat cgacaacgct tgatgctaca aatgtgatga catacgaacc agttgtgaaa
     9001 cctgaatacc aaactgtcaa tgctgctaaa acagcaacgg taacgattgc taaaggacaa
     9061 tcatttagta ttggtgatat taaacaatat tttactttaa gtaatggaca acctattcca
     9121 agtggcacat ttacaaatat tacatctgat agaactattc caactgcaca agaagttagt
     9181 caaatgaacg caggcacgca gttataccat ataactgcta caaatgcgta tcataaagat
     9241 agtgaagact tctatattag tttgaaaatc atcgatgtga aacaaccaga aggcgatcaa
     9301 cgtgtatatc gtacatcaac atatgattta actactgatg aaatctcaaa agtaaaacaa
     9361 gcatttatta atgcaaatag agatgtaatt acgcttgccg aaggtgatat ttcagttaca
     9421 aatacaccta atggtgctaa tgtaagtact attacagtaa atattaataa aggtcgatta
     9481 acgaaatcat tcgcgtcaaa cctagctaat atgaatttct tgcgttgggt taatttccca
     9541 caagattata cagtgacatg gacgaatgca aaaattgcaa acagaccaac agatggtggt
     9601 ttatcatggt ctgatgacca taaatcttta atttatcgtt atgatgctac attaggtact
     9661 caaattacga cgaatgatat tttaacaatg ttaaaagcaa caactacagt gcctggattg
     9721 cgaaataaca ttactggtaa tgaaaaatca caagcagaag ctggcggaag acctaacttt
     9781 agaacgactg gttattcaca atcaaatgcg acaactgatg gtcaacgtca atttacgttg
     9841 aatggtcaag tgattcaagt gttagacatc atcaaccctt caaacggtta tggtgggcaa
     9901 cctgttacaa attcaaatac tcgtgcaaac catagtaact caactgttgt taacgtaaac
     9961 gaaccggcag ctaatggtgc tggcgcattt acaattgacc acgttgtaaa aagtaattct
    10021 acacataatg caagtgatgc agtttataaa gcacagttat acttaacgcc atatggtcca
    10081 aaacaatatg ttgaacattt aaatcaaaat acaggaaata ctactgacgc tattaacatt
    10141 tattttgtac caagtgactt agtgaatcca acaatttcag taggtaatta cactaatcat
    10201 caagtgttct caggtgaaac atttacaaat actattacag cgaatgataa ctttggtgtg
    10261 caatctgtaa ctgtaccaaa tacatcacaa attacaggta ctgttgataa taaccatcaa
    10321 catgtttctg caacggcacc aaatgtgaca tcagcaacta ataagacaat caatttatta
    10381 gcaactgata caagcggcaa tacagctaca acttcgttca atgtaacagt gaaacctttg
    10441 cgtgataaat atcgagttgg tacttcatca acggctgcta atcctgtgag aattgccaat
    10501 atttcgaata atgcgacagt atcacaagct gatcaaacga caattattaa ttcgttaacg
    10561 tttactgaaa cagtaccaaa tagaagttat gcaagagcaa gtgcgaatga aatcactagt
    10621 aaaacagtta gtaatgtcag tcgtactgga aataatgcca atgtcacagt aactgttact
    10681 tatcaagatg gaacaacatc aacagtgact gtacctgtaa agcatgtcat tccagaaatc
    10741 gttgcacatt cgcattacac tgtacaaggc caagacttcc cagcaggtaa tggttctagt
    10801 gcatcagatt actttaagtt atctaatggt agtgacattg cagatgcaac tattacatgg
    10861 gtaagtggac aagcgccaaa taaagataat acacgtattg gtgaagatat aactgtaact
    10921 gcacatatct taattgatgg cgaaacaacg ccgattacga aaacagcaac atataaagta
    10981 gtaagaactg taccgaaaca tgtctttgaa acagccagag gtgttttata cccaggtgtt
    11041 tcagatatgt atgatgcgaa acaatatgtt aagccagtaa ataattcttg gtcgacaaat
    11101 gcgcaacata tgaatttcca atttgttgga acatatggtc ctaacaaaga tgttgtaggc
    11161 atatctactc gtcttattag agtgacatat gataatagac aaacagaaga tttaactatt
    11221 ttatctaaag ttaaacctga cccacctaga attgacgcaa actctgtgac atataaagca
    11281 ggtcttacaa accaagaaat taaagttaat aacgtattaa ataactcgtc agtaaaatta
    11341 tttaaagcag ataatacacc attaaatgtc acaaatatta ctcatggtag cggttttagt
    11401 tcggttgtga cagtaagtga cgcgttacca aatggcggaa ttaaagcaaa atcttcaatt
    11461 tcaatgaaca atgtgacgta tacgacgcaa gacgaacatg gtcaagttgt tacagtaaca
    11521 agaaatgaat ctgttgattc aaatgacagt gcaacagtaa cagtgacacc acaattacaa
    11581 gcaactactg aaggcgctgt atttattaaa ggtggcgacg gttttgattt cggacacgta
    11641 gaaagattta ttcaaaaccc gccacatggg gcaacggttg catggcatga tagtccagat
    11701 acatggaaga atacagtcgg taacactcat aaaactgcgg ttgtaacatt acctaatggt
    11761 caaggtacgc gtaatgttga agttccagtc aaagtttatc cagttgctaa tgcaaaggcg
    11821 ccatcacgtg atgtgaaagg tcaaaatttg actaatggaa cggatgcgat gaactacatt
    11881 acatttgatc caaatacaaa cacaaatggt atcactgcag catgggcaaa tagacaacaa
    11941 ccaaataacc aacaagcagg cgtgcaacat ttaaatgtcg atgtcacata tccaggtatt
    12001 tcagctgcta aacgagttcc tgttactgtt aatgtatatc aatttgaatt ccctcaaact
    12061 acttatacga caacggttgg aggcacttta gcaagtggta cgcaagcatc aggatatgca
    12121 catatgcaaa atgctactgg tttaccaaca gatggattta cgtataaatg gaatcgtgat
    12181 actacaggta caaatgacgc aaactggtca gctatgaata aaccgaatgt ggctaaagtc
    12241 gttaacgcaa aatatgacgt catctataac ggacatactt ttgcaacatc tttaccagcg
    12301 aaatttgtag taaaagatgt gcaaccagcg aaaccaactg tgactgaaac agcggcagga
    12361 gcgattacaa ttgcacctgg agcaaaccaa acagtgaata cacatgccgg taacgtaacg
    12421 acatacgctg ataaattagt tattaaacgt aatggtaacg ttgtgacgac atttacacgt
    12481 cgcaataata cgagtccatg ggtgaaagaa gcatctgcag caactgtagc aggtattgct
    12541 ggaactaata atggtattac tgttgcagca ggtactttca accctgctga tacaattcaa
    12601 gttgttgcaa cgcaaggaag cggagagaca gtgagtgatg agcaacgtag tgatgatttc
    12661 acagttgtcg caccacaacc gaaccaagcg actactaaga tttggcaaaa tggtcatatt
    12721 gatatcacgc ctaataatcc atcaggacat ttaattaatc caactcaagc aatggatatt
    12781 gcttacactg aaaaagtggg taatggtgca gaacatagta agacaattaa tgttgttcgt
    12841 ggtcaaaata atcaatggac aattgcgaat aagcctgact atgtaacgtt agatgcacaa
    12901 actggtaaag tgacgttcaa tgccaatact ataaaaccaa attcatcaat cacaattact
    12961 ccgaaagcag gtacaggtca ctcagtaagt agtaatccaa gtacattaac tgcaccggca
    13021 gctcatactg tcaacacaac tgaaattgtg aaagattatg gttcaaatgt aacagcagct
    13081 gaaattaaca atgcagttca agttgctaat aaacgtactg caacgattaa aaatggcaca
    13141 gcaatgccta ctaatttagc tggtggtagc acaacgacga ttcctgtgac agtaacttac
    13201 aatgatggta gtactgaaga agtacaagag tccattttca caaaagcgga taaacgtgag
    13261 ttaatcacag ctaaaaatca tttagatgat ccagtaagca ctgaaggtaa aaagccaggt
    13321 acaattacgc agtacaataa tgcaatgcat aatgcgcaac aacaaatcaa tactgcgaaa
    13381 acagaagcac aacaagtgat taataatgag cgtgcaacac cacaacaagt ttctgacgca
    13441 ctaactaaag ttcgtgcagc acaaactaag attgatcaag ctaaagcatt acttcaaaat
    13501 aaagaagata atagccaatt agtaacgtct aaaaataact tacaaagttc tgtgaaccaa
    13561 gtaccatcaa ctgctggtat gacgcaacaa agtattgata actataatgc gaagaagcgt
    13621 gaagcagaaa ctgaaataac tgcagctcaa cgtgttattg acaatggcga tgcaactgca
    13681 caacaaattt cagatgaaaa acatcgtgtc gataacgcat taacagcatt aaaccaagcg
    13741 aaacatgatt taactgcaga tacacatgcc ttagagcaag cagtgcaaca attgaatcgc
    13801 acaggtacaa cgactggtaa gaagccggca agtattactg cttacaataa ttcgattcgt
    13861 gcacttcaaa gtgacttaac aagtgctaaa aatagcgcta atgctattat tcaaaagcca
    13921 ataagaacag tacaagaagt gcaatctgcg ttaacaaatg taaatcgtgt caatgagcga
    13981 ttaacgcaag caattaatca attagtacct ttagctgata atagtgcttt aaaaactgct
    14041 aagacgaaac ttgatgaaga aatcaataaa tcagtaacta ctgatggtat gacacaatca
    14101 tcaatccaag catatgaaaa tgctaaacgt gcgggtcaaa cagaatcaac aaatgcacaa
    14161 aatgttatta acaatggtga tgcgactgac caacaaattg ccgcagaaaa aacaaaagta
    14221 gaagaaaaat ataatagctt aaaacaagca attgctggat taactccaga cttggcacca
    14281 ttacaaactg caaaaactca gttgcaaaat gatattgatc agccaacgag tacgactggt
    14341 atgacaagcg catctattgc agcatttaat gaaaaacttt cagcagctag aactaaaatt
    14401 caagaaattg atcgtgtatt agcctcacat ccagatgttg cgacaatacg tcaaaacgtg
    14461 acagcagcga atgccgctaa atcagcactt gatcaagcac gtaatggctt aacagtcgat
    14521 aaagcgcctt tagaaaatgc gaaaaatcaa ctacaacata gtattgacac gcaaacaagt
    14581 acaactggta tgacacaaga ctctataaat gcatacaatg cgaagttaac agctgcacgt
    14641 aataagattc aacaaatcaa tcaagtatta gcaggttcac cgactgtaga acaaattaat
    14701 acaaatacgt ctacagcaaa tcaagctaaa tctgatttag atcatgcacg tcaagcttta
    14761 acaccagata aagcgccgct tcaaactgcg aaaacgcaat tagaacaaag cattaatcaa
    14821 ccaacggata caacaggtat gacgaccgct tcgttaaatg cgtacaacca aaaattacaa
    14881 gcagcgcgtc aaaagttaac tgaaattaat caagtgttga atggcaaccc aactgtccaa
    14941 aatatcaatg ataaagtgac agaggcaaac caagctaagg atcaattaaa tacagcacgt
    15001 caaggtttaa cattagatag acagccagcg ttaacaacat tacatggtgc atctaactta
    15061 aaccaagcac aacaaaataa tttcacgcaa caaattaatg ctgctcaaaa tcatgctgcg
    15121 cttgaaacaa ttaagtctaa cattacggct ttaaatactg cgatgacgaa attaaaagac
    15181 agtgttgcgg ataataatac aattaaatca gatcaaaatt acactgacgc aacaccagct
    15241 aataaacaag cgtatgataa tgcagttaat gcggctaaag gtgtcattgg agaaacgact
    15301 aatccaacga tggatgttaa cacagtgaac caaaaagcag catctgttaa atcgacgaaa
    15361 gatgctttag atggtcaaca aaacttacaa cgtgcgaaaa cagaagcaac aaatgcgatt
    15421 acgcatgcaa gtgatttaaa ccaagcacaa aagaatgcat taacacaaca agtgaatagt
    15481 gcacaaaacg tgcaagcagt aaatgatatt aaacaaacga ctcaaagctt aaatactgct
    15541 atgacaggtt taaaacgtgg cgttgctaat cataaccaag tcgtacaaag tgataattat
    15601 gtcaacgcag atactaataa gaaaaatgat tacaacaatg catacaacca tgcgaatgac
    15661 attattaatg gtaatgcaca acatccagtt ataacaccaa gtgatgttaa caatgcttta
    15721 tcaaatgtca caagtaaaga acatgcattg aatggtgaag ctaagttaaa tgctgcgaaa
    15781 caagaagcga atactgcatt aggtcattta aacaatttaa ataatgcaca acgtcaaaac
    15841 ttacaatcgc aaattaatgg tgcgcatcaa attgatgcag ttaatacaat taagcaaaat
    15901 gcaacaaact tgaatagtgc aatgggtaac ttaagacaag ctgttgcaga taaagatcaa
    15961 gtgaaacgta cagaagatta tgcggatgca gatacagcta aacaaaatgc atataacagt
    16021 gcagtttcaa gtgccgaaac aatcattaat caaacaacaa atccaacgat gtctgttgat
    16081 gatgttaatc gtgcaacttc agctgttact tctaataaaa atgcattaaa tggttatgaa
    16141 aaattagcac aatctaaaac agatgctgca agagcaattg atgcattacc acatttaaat
    16201 aatgcacaaa aagcagatgt taaatctaaa attaatgctg catcaaatat tgctggcgta
    16261 aatactgtta aacaacaagg tacagattta aatacagcga tgggtaactt gcaaggtgca
    16321 atcaatgatg aacaaacgac gcttaatagt caaaactatc aagatgcgac acctagtaag
    16381 aaaacagcat acacaaatgc ggtacaagct gcgaaagata ttttaaataa atcaaatggt
    16441 caaaataaaa cgaaagatca agttactgaa gcgatgaatc aagtgaattc tgctaaaaat
    16501 aacttagatg gtacgcgttt attagatcaa gcgaagcaaa cagcaaaaca gcagttaaat
    16561 aatatgacgc atttaacaac tgcacaaaaa acgaatttaa caaaccaaat taatagtggt
    16621 actactgtcg ctggtgttca aacggttcaa tcaaatgcca atacattaga tcaagccatg
    16681 aatacgttaa gacaaagtat tgccaacaaa gatgcgacta aagcaagtga agattacgta
    16741 gatgctaata atgataagca aacagcatat aacaacgcag tagctgctgc tgaaacgatt
    16801 attaatgcta atagtaatcc agaaatgaat ccaagtacga ttacacaaaa agcagagcaa
    16861 gtgaatagtt ctaaaacggc acttaacggt gatgaaaact tagctgctgc aaaacaaaat
    16921 gcgaaaacgt acttaaacac attgacaagt attacagatg ctcaaaagaa caatttgatt
    16981 agtcaaatta ctagtgcgac aagagtgagt ggtgttgata ctgtaaaaca aaatgcgcaa
    17041 catctagacc aagctatggc tagcttacag aatggtatta acaacgaatc tcaagtgaaa
    17101 tcatctgaga aatatcgtga tgctgataca aataaacaac aagagtatga taatgctatt
    17161 actgcagcga aagcgatttt aaataaatcg acaggtccaa acactgcgca aaatgcagtt
    17221 gaagcagcat tacaacgtgt taataatgcg aaagatgcat tgaatggtga tgcaaaatta
    17281 attgcagctc aaaacgcagc gaaacaacat ttaggtactt taacgcatat cactacagct
    17341 caacgtaatg atttaacaaa tcaaatttca caagctacaa acttagctgg tgttgaatct
    17401 gttaaacaaa atgcgaatag tttagatggt gctatgggta acttacaaac ggctatcaac
    17461 gataagtcag gaacattagc gagccaaaac ttcttggatg ctgatgagca aaaacgtaat
    17521 gcatacaatc aagctgtatc agcagccgaa accattttaa ataaacaaac tggaccgaat
    17581 acagcgaaaa cagcagtcga acaagcactt aataatgtta ataatgcgaa acatgcatta
    17641 aatggtacgc aaaacttaaa caatgcgaaa caagcagcga ttacagcaat caatggcgca
    17701 tctgatttaa atcaaaaaca aaaagatgca ttaaaagcac aagctaatgg tgctcaacgc
    17761 gtatctaatg cacaagatgt acagcacaat gcgactgaac tgaacacggc aatgggcaca
    17821 ttaaaacatg ccatcgcaga taagacgaat acgttagcaa gcagtaaata tgttaatgcc
    17881 gatagcacta aacaaaatgc ttacacaact aaagttacca atgctgaaca tattattagc
    17941 ggtacgccaa cggttgttac gacaccttca gaagtaacag ctgcagctaa tcaagtaaac
    18001 agcgcgaaac aagaattaaa tggtgacgaa agattacgtg aagcaaaaca aaacgccaat
    18061 actgctattg atgcattaac acaattaaat acacctcaaa aagctaaatt aaaagaacaa
    18121 gtgggacaag ccaatagatt agaagacgta caaactgttc aaacaaatgg acaagcattg
    18181 aacaatgcaa tgaaaggctt aagagatagt attgctaacg aaacaacagt caaaacaagt
    18241 caaaactata cagacgcaag tccgaataac caatcaacat ataatagcgc tgtgtcaaat
    18301 gcgaaaggta tcattaatca aactaacaat ccgactatgg atactagtgc gattacccaa
    18361 gctacaacac aagtgaataa tgctaaaaat ggtttaaacg gtgctgaaaa cttaagaaat
    18421 gcacaaaaca ctgctaagca aaacttaaat acattatcac acttaacaaa taaccaaaaa
    18481 tctgccatct catcacaaat tgatcgtgca ggtcatgtga gtgaggtaac tgctactaaa
    18541 aatgcagcaa ctgagttgaa tacgcaaatg ggtaacttgg aacaagctat ccatgatcaa
    18601 aacacagtta aacaaagtgt taaatttact gatgcagata aagctaaacg tgatgcgtat
    18661 acaaatgcgg taagcagagc tgaagcaatt ctgaataaaa cgcaaggtgc aaatacgtct
    18721 aaacaagatg ttgaagcggc tattcaaaat gtttcaagtg ctaaaaatgc attgaatggt
    18781 gatcaaaacg ttacaaatgc gaagaatgca gctaaaaatg cattaaataa cttaacgtca
    18841 attaataatg cacaaaaacg tgacttaaca actaaaattg atcaagcaac aactgtagct
    18901 ggtgttgaag ctgtatctaa tacgagtaca caattgaata cagcgatggc taacttgcaa
    18961 aatggtatta atgataaaac aaatacacta gcaagtgaaa actatcatga tgctgattca
    19021 gataagaaaa ctgcttatac tcaagccgtt acgaacgcag aaaatatttt aaataaaaat
    19081 agtggatcaa atttagacaa aactgccgtt gaaaacgcgt tgtcacaagt tgctaatgcg
    19141 aaaggtgccc taaatggtaa ccataattta gagcaagcta aatcaaatgc aaacactact
    19201 ataaacggac ttcaacattt aacaactgct caaaaagata aattgaaaca acaagtgcaa
    19261 caagcacaaa atgttgcagg tgtagatact gttaaatcaa gtgccaacac attaaatggt
    19321 gctatgggta cgttaagaaa tagcatacaa gataacacag ctacgaaaaa tggccaaaac
    19381 tatcttgatg ctacagaacg taacaaaaca aactataaca atgctgttga tagtgctaat
    19441 ggtgtcatta atgcaacaag caatccaaat atggatgcta atgcaattaa ccaaatcgct
    19501 acacaagtga catcaacgaa aaatgcatta gatggtacac ataatttaac gcaagcgaaa
    19561 caaacagcaa caaatgccat cgatggtgct actaacttaa ataaagcgca aaaagatgcg
    19621 ttaaaagcac aagttacaag tgcgcaacgt gttgcaaatg taacaagtat ccaacaaact
    19681 gcaaatgaac ttaatacagc tatgggtcaa ttacaacatg gtattgatga tgaaaatgca
    19741 acaaaacaaa ctcaaaaata tcgtgacgct gaacaaagta agaaaactgc ttatgatcaa
    19801 gctgtagctg ctgcgaaagc aattttaaat aaacaaacag gttcaaattc agataaagca
    19861 gcagttgacc gtgcattaca acaagtaaca agtacgaaag atgcattgaa tggtgatgca
    19921 aaactggcag aagcgaaagc ggcagctaaa caaaacttag gcactttaaa ccatattacg
    19981 aatgcacaac gtactgactt agaaggccaa atcaatcaag cgacgactgt tgatggcgtt
    20041 aatactgtaa aaacaaatgc caatacatta gacggcgcaa tgaatagctt acaaggttca
    20101 atcaatgata aagatgcgac attaagaaat caaaattatc ttgatgcgga tgaatcaaaa
    20161 cgaaatgcat atacgcaagc tgtcacagcg gctgaaggca ttttaaataa acaaactggt
    20221 ggtaacacat ctaaagcaga cgttgataat gcattaaatg cagttacaag agcgaaagcg
    20281 gctttaaatg gtgctgacaa cttaagaaat gcgaaaactt cagcaacaaa tacgattgat
    20341 ggtttaccta acttaacaca attacaaaaa gacaacttga agcatcaagt tgaacaagcg
    20401 caaaatgtag caggtgtaaa tggtgttaaa gataaaggta atacgttaaa tactgccatg
    20461 ggtgcattac gtacaagtat ccaaaatgat aatacgacga aaacaagtca aaattatctt
    20521 gatgcatctg acagcaacaa aaataattac aatactgctg taaataatgc aaatggtgtt
    20581 attaatgcaa cgaacaatcc aaatatggat gctaatgcga ttaatggcat ggcaaatcaa
    20641 gtcaatacaa caaaagcagc gttaaatggt gcacaaaact tagctcaagc taaaacaaat
    20701 gcgacgaaca caattaacaa cgcacatgac ttaaaccaaa aacaaaaaga tgcattaaaa
    20761 acacaagtta acaatgcaca acgtgtatct gatgcaaata acgttcaaca cactgcaact
    20821 gaattgaaca gtgcgatgac agcacttaaa gcagctattg ctgataaaga aagaacaaaa
    20881 gcaagcggta attatgtcaa tgctgatcaa gaaaaacgtc aagcgtatga ttcaaaagtg
    20941 actaacgctg aaaatatcat tagtggtaca ccgaatgcga cattaacagt caatgacgta
    21001 aatagtgcgg catcacaagt caatgcggct aaaacagcat taaatggtga taacaactta
    21061 cgtgtagcga aagagcatgc caacaataca attgacggct tagcacaatt gaataatgca
    21121 caaaaagcaa aattaaaaga acaagttcaa agtgcaacta cattagatgg tgttcaaact
    21181 gttaaaaata gttctcaaac gttgaataca gcgatgaaag gcttaagaga tagtattgcg
    21241 aatgaagcaa caattaaagc aggtcaaaac tacactgacg caagtccaaa taatcgtaac
    21301 gagtacgaca gtgcagttac tgcagcaaaa gcaatcatta atcaaacatc gaacccaacg
    21361 atggaaccaa atactattac gcaagtaaca tcacaagtga caactaaaga acaggcatta
    21421 aatggtgcgc gaaacttagc tcaagctaag acaactgcga aaaacaactt gaataactta
    21481 acatcaatta acaatgcaca aaaagatgcg ttaacgcgta gcattgatgg tgcaacaaca
    21541 gtagctggtg taaatcaaga aactgcaaaa gcaacagaat taaataacgc aatgcatagt
    21601 ttacaaaatg gtatcaatga tgagacacaa acaaaacaaa ctcagaaata cctagatgca
    21661 gagccaagta agaaatcagc ttatgatcaa gcagtaaatg cagcgaaagc aattttaaca
    21721 aaagctagtg gtcaaaatgt agacaaagca gcagttgaac aagcattgca aaatgtgaac
    21781 agtacgaaga cggcgttgaa cggtgatgcg aaattaaatg aagctaaagc agctgcgaaa
    21841 caaacgttag gtacattaac acacattaat aatgcacaac gtacagcgtt agacaatgaa
    21901 attacacaag caacaaatgt tgaaggtgtt aatacagtta aagccaaagc gcaacaatta
    21961 gatggtgcta tgggtcaatt agaaacatca attcgtgata aagacacgac gttacaaagt
    22021 caaaattatc aagatgctga tgatgctaaa cgaactgctt attctcaagc agtaaatgca
    22081 gcagcaacta ttttaaataa aacagctggc ggtaatacac ctaaagcaga tgttgaaaga
    22141 gcaatgcaag ctgttacaca agcaaatact gcattaaacg gtattcaaaa cttagatcgt
    22201 gcgaaacagg ctgctaacac agcgattaca aatgcttcgg acttaaatac aaaacaaaaa
    22261 gaagcattaa aagcacaagt aacaagtgca ggacgtgtat ctgcagcaaa tggtgttgaa
    22321 catactgcga ctgaattaaa tactgcgatg acagctttaa agcgtgccat tgctgataaa
    22381 gctgagacaa aagctagtgg taactatgtc aatgctgatg cgaataaacg tcaagcatat
    22441 gatgaaaaag ttacagctgc cgaaaatatc gttagtggta caccaacacc aacgttaaca
    22501 ccagcagatg ttacaaatgc agcaacgcaa gtaacgaatg ctaagacgca gttaaacggt
    22561 aatcataatt tagaagtagc gaaacaaaat gctaacactg caattgatgg tttaacttct
    22621 ttaaatggtc cgcaaaaagc aaaacttaaa gaacaagtgg gtcaagcgac gacgttgcca
    22681 aatgttcaaa ctgttcgtga taatgcacaa acattaaaca ctgcaatgaa aggtctacga
    22741 gatagcattg cgaatgaagc aacgattaaa gcaggtcaaa actacacaga tgcaagtcaa
    22801 aacaaacaaa ctgactacaa cagtgcagtc actgcagcaa aagcaatcat tggtcaaaca
    22861 actagtccat caatgaatgc gcaagaaatt aatcaagcga aagaccaagt gacagctaaa
    22921 caacaagcgt taaacggtca agaaaactta agaactgcgc aaacaaatgc gaagcaacat
    22981 ttgaacggct taagtgactt aactgacgct caaaaagatg cagtgaaacg tcaaatcgaa
    23041 ggtgcaacgc atgttaatga agtaacacaa gcacaaaata atgcggatgc attaaataca
    23101 gctatgacga acttgaaaaa tggtattcaa gatcagaata cgattaagca aggtgttaac
    23161 ttcactgatg ccgacgaagc gaaacgtaat gcatatacaa atgcagtgac gcaagctgaa
    23221 caaattttaa ataaagcaca aggtccaaat acttcaaaag acggtgtcga aactgcgtta
    23281 gaaaatgtac aacgtgctaa aaacgaattg aacggtaatc aaaatgttgc gaacgctaag
    23341 acaactgcga aaaatgcatt gaataaccta acatcaatta ataatgcaca aaaagaagca
    23401 ttgaaatcac aaattgaagg tgcgacaaca gttgcaggtg taaatcaagt gtctacaacg
    23461 gcatctgaat taaatacagc aatgagcaac ttacaaaatg gtattaatga tgaagcagct
    23521 acaaaagcag ctcagaaata tactgatgca gatagagaaa aacaaactgc atacaatgac
    23581 gctgtaacag cagctaaaac gttattagat aaaacagctg gttcaaatga caataaagca
    23641 gctgttgaac aagcattaca acgtgtgaat actgctaaaa cagcattaaa tggtgacgag
    23701 cgattaaatg aagcgaagaa cacagctaaa caacaagtag cgacaatgtc acacttaact
    23761 gatgctcaaa aagcaaactt aacatcgcaa atcgaaagtg gtacgactgt tgcaggtgtt
    23821 caaggtattc aagctaatgc cggtacttta gatcaagcaa tgaatcaatt aagacaaagt
    23881 attgcttcta aagatgcgac taaatcaagc gaagattatc aagacgcgaa tgcagattta
    23941 caaaatgcat acaatgatgc ggtaactaat gctgaaggta ttattagtgc aacgaataac
    24001 cctgaaatga atcctgatac aattaaccaa aaagcgagcc aagtgaacag tgcgaagtct
    24061 gcattgaacg gtgatgaaaa attagcagca gcaaaacaaa ctgcgaaatc agatatcggt
    24121 cgtttgacag acttgaacaa tgcacaacga actgcggcaa atgctgaagt ggatcaagca
    24181 ccaaatcttg cagctgtcac agcggctaaa aataaagcaa catcgttaaa cacagcgatg
    24241 ggtaatttga aacatgcact tgctgaaaag gataatacga aacgtagtgt caattacaca
    24301 gatgcggatc aaccaaaaca acaagcgtat gatactgcgg ttacacaagc agaagcaatt
    24361 actaatgcta atggcagcaa cgcgaatgaa acacaagttc aagcagcact aaaccaattg
    24421 aatcaagcta aaaatgactt gaatggtgat aataaagttg ctcaagcaaa agagtcagcg
    24481 aaacgtgcat tagcttcata tagtaacttg aataatgcgc aatcaactgc agcaattagt
    24541 caaattgaca atgcaacgac agtagcaggc gtaactgctg cacaaaatac tgctaatgaa
    24601 ttaaatacag caatgggtca acttcaaaat ggtataaatg accaaaacac tgttaaacaa
    24661 caagtgaact ttacagatgc tgaccaaggt aagaaagatg cttacacaaa tgctgttacg
    24721 aatgctcaag gtattttaga taaagcacac ggtcaaaata tgacgaaagc acaagttgaa
    24781 gctgcattaa atcaagtaac gactgctaag aatgctttaa acggtgacgc aaatgtaaga
    24841 caagcaaaat cagatgcgaa agcaaactta ggtacattaa cacacttaaa taatgcacaa
    24901 aaacaagatt taacatcaca aatcgaaggc gcaacaacag tcaacggtgt aaatggtgtt
    24961 aaaacgaaag cacaagactt agatggtgca atgcaacgat tacaatcagc tatagcaaat
    25021 aaagatcaaa ctaaagcgag cgaaaattac atcgacgcag atccaactaa gaaaacagca
    25081 tttgataatg ctatcacaca agctgaatct tacttaaata aagatcacgg tgcgaataaa
    25141 gataagcaag ctgttgaaca agcaattcaa agtgtaacgt ctactgaaaa tgctttgaac
    25201 ggtgacgcga acttacaacg cgctaaaact gaagctatac aagctatcga taacttgaca
    25261 catttgaata caccacaaaa aacagcatta aaacaacaag tgaacgctgc gcaacgtgta
    25321 tcaggtgtaa ctgatctgaa aaatagtgct acatcactta ataatgcgat ggatcaatta
    25381 aaacaagcaa ttgctgatca tgacacaatt gtagctagtg gtaattacac taacgcgagt
    25441 cctgataagc aaggtgctta tactgatgca tataatgctg cgaaaaacat tgtaaatggt
    25501 tcgcctaatg tgattacaaa tgcagcagat gttacagcag caacacaacg tgttaataat
    25561 gctgaaacag gtttaaacgg tgatacaaac ttagcaactg cgaagcaaca agctaaagat
    25621 gcattacgtc aaatgacaca tttatctgat gcacaaaaac aaagtattac tggtcaaatt
    25681 gatagcgcga cacaagtaac tggcgttcaa agtgtgaaag acaacgcgac aaatcttgat
    25741 aatgcaatga atcaacttcg aaatagtatt gcgaataaag atgatgtaaa agcgagtcaa
    25801 ccatatgttg atgcagatag agataaacaa aatgcataca atacagcagt tacaaatgct
    25861 gaaaatatca ttaatgcaac gagtcagccg acacttgatc catctgcagt aacacaagca
    25921 gctaatcaag tgagcactaa caaaactgcg cttaatggtg cacaaaactt agcgaataaa
    25981 aagcaagaaa cgactgctaa catcaaccaa ttaagtcatt taaataatgc tcaaaagcaa
    26041 gatttaaata cgcaagtgac aaatgcacca aatattagca cagtaaatca agtgaaaact
    26101 aaagctgaac aattagatca agcaatggaa cgtttaatca acggaatcca agacaaagat
    26161 caagtgaaac aaagtgttaa ctttacagat gcagatccag aaaaacaaac agcatacaac
    26221 aatgcggtaa ctgctgctga aaatattatt aatcaagcaa atggtacaaa tgcgaaccaa
    26281 tcacaagttg aagcagcact ttcaactgta acaactacga aacaagcgtt gaatggtgat
    26341 agaaaagtaa cagatgctaa aaacaatgca aaccaaacat tatctacgtt agataactta
    26401 aacaatgcac aaaaaggtgc tgttactgga aacatcaatc aagcgcacac tgtagctgaa
    26461 gtaacgcaag ccattcaaac cgctcaggaa ctgaatacag cgatgggtaa cttgaaaaat
    26521 agcttgaatg ataaagacac tacacttggc agtcaaaact ttgcagatgc agatccagag
    26581 aagaaaaatg catacaatga agcggttcat aatgctgaaa atattttaaa taaatctaca
    26641 ggtacgaacg tgcctaaaga tcaagttgaa gcagctatga atcaagtgaa tgctacaaaa
    26701 gcagcgctta atggtactca aaaccttgaa aaagctaaac aacacgcaaa tacagcaatt
    26761 gacggtttaa gccatttaac aaatgcacaa aaagaggcat taaaacaatt ggtacaacaa
    26821 tcgactactg ttgcagaagc acaaggtaat gagcaaaaag caaacaatgt tgatgcagca
    26881 atggacaaat tacgtcaaag tattgcagat aatgcgacaa caaaacaaaa ccaaaattat
    26941 actgatgcaa gtcagaataa aaaggatgcg tacaataatg ctgtcacaac tgcacaaggt
    27001 attattgatc aaactacaag tccaacttta gatccgactg ttatcaatca agctgctgga
    27061 caagtaagca caactaaaaa tgcattaaat ggtaatgaaa acctagaggc agcgaaacaa
    27121 caagcgtcac aatcattagg ttcattagat aacttaaata atgcgcaaaa acaaacagtt
    27181 actgatcaaa ttaatggcgc gcatactgtt gatgaagcaa atcaaattaa gcaaaatgcg
    27241 caaaacttaa atacagcgat gggtaacttg aaacaagcga tagctgacaa agatgctacg
    27301 aaagcgacag ttaacttcac tgatgcagat caagcaaaac aacaagcata taacactgct
    27361 gttacaaatg ctgaaaatat catttcaaaa gctaatggcg gcaatgcaac acaagctgaa
    27421 gttgaacaag caatcaaaca agttaatgct gcaaaacaag cattaaatgg taatgccaac
    27481 gttcaacatg caaaagacga agcaacagca ttaattaata gctctaatga ccttaaccaa
    27541 gcacaaaaag acgcattaaa acaacaagtt caaaatgcaa ctactgtagc tggtgtaaac
    27601 aatgttaaac aaacagcaca agagttaaac aatgctatga cacaattaaa acaaggcatt
    27661 gcagataaag aacaaacaaa agctgatggt aactttgtca atgcagatcc tgataagcaa
    27721 aatgcatata atcaagcagt agcgaaagct gaagcattaa ttagtgctac gcctgatgtt
    27781 gtcgttacac ctagcgaaat tactgcagcg ttaaataaag ttacgcaagc taaaaatgat
    27841 ttaaatggta atacaaactt agcaacggcg aaacaaaatg ttcaacatgc tattgatcaa
    27901 ttgccaaact taaaccaagc gcaacgtgat gaatacagca aacaaatcac gcaagcaaca
    27961 cttgtaccaa acgtcaatgc tattcaacaa gcggcgacaa cgcttaatga cgcgatgaca
    28021 caattgaaac aaggtattgc gaataaagca caaattaaag gtagcgagaa ctatcacgat
    28081 gctgatactg acaagcaaac agcatatgat aatgcagtaa caaaagcaga agaattgtta
    28141 aaacaaacaa caaatccaac aatggatcca aatacaattc aacaagcatt aactaaagtg
    28201 aatgacacaa atcaagcact taacggtaat caaaaattag ctgatgccaa acaagatgct
    28261 aagacaacac ttggtacact agatcattta aatgatgctc aaaaacaagc gctaacaact
    28321 caagttgaac aagcaccaga tattgcaaca gttaataatg ttaagcaaaa tgctcaaaat
    28381 ctgaataatg ctatgactaa cttaaacaat gcattacaag ataaaactga gacattaaat
    28441 agcattaact ttactgatgc agatcaagct aagaaagatg cttatactaa tgcggtttca
    28501 catgcagaag gtattttatc taaagcaaat ggcagcaatg caagtcaaac tgaagtggaa
    28561 caagcgatgc aacgtgtgaa cgaagcgaaa caagcattga atggtaatga caatgtacaa
    28621 cgtgcaaaag atgcagcgaa acaagtgatt acaaatgcaa atgatttaaa tcaagcgcaa
    28681 aaagatgcat taaaacaaca agtcgatgct gcgcaaactg ttgcaaatgt aaacacgatt
    28741 aagcaaacag cacaagattt aaatcaagca atgacacaat tgaaacaagg tattgcagat
    28801 aaagaccaaa ctaaagcaaa tggtaacttt gtcaatgctg atactgataa gcaaaatgct
    28861 tacaacaatg cggtagcaca tgctgaacaa ataattagtg gtacaccaaa tgcaaacgtg
    28921 gatccacaac aagtggctca agcgttacaa caagtgaatc aagctaaggg tgatttaaac
    28981 ggtaaccata acttacaagt tgctaaagac aatgcaaata cagccattga tcagttacca
    29041 aacttaaatc aaccacaaaa aacagcatta aaagaccaag tgtcgcatgc agaacttgtt
    29101 acaggtgtta atgctattaa gcaaaatgct gatgcgttaa ataatgcaat gggtacattg
    29161 aaacaacaaa ttcaagcgaa cagtcaagta ccacagtcag ttgactttac acaagcggat
    29221 caagacaaac aacaagcata taacaatgcg gctaaccaag cgcaacaaat cgcaaatggc
    29281 ataccaacac ctgtattgac gcctgataca gtaacacaag cagtgacaac tatgaatcaa
    29341 gcgaaagatg cattaaacgg tgatgaaaaa ttagcacaag cgaaacaaga agctttagca
    29401 aatcttgata cgttacgcga tttaaatcaa ccacaacgtg atgcattacg taaccaaatc
    29461 aatcaagcac aagcgttagc tacagttgaa caaactaaac aaaatgcaca aaatgtgaat
    29521 acagcaatga gtaacttgaa acaaggtatt gcaaacaaag atactgtcaa agcaagtgag
    29581 aactatcatg atgctgatgc cgataagcaa acagcatata caaatgcagt gtctcaagcg
    29641 gaaggtatta tcaatcaaac gacaaatcca acgcttaacc cagatgaaat aacacgtgca
    29701 ttaactcaag tgactgatgc taaaaatggc ttaaacggtg aagctaaatt ggcaactgaa
    29761 aagcaaaatg ctaaagatgc cgtaagtggg atgacgcatt taaacgatgc tcaaaaacaa
    29821 gcattaaaag gtcaaatcga tcaatcgcct gaaattgcta cagtgaacca agttaaacaa
    29881 acagcaacga gcctagatca agcaatggat caattatcac aagctattaa tgataaagct
    29941 caaacattag cggacggtaa ttacttaaat gcagatcctg acaaacaaaa tgcgtataaa
    30001 caggcagtag caaaagctga agcattattg aataaacaaa gtggtactaa tgaagtacaa
    30061 gcacaagttg aaagcatcac taatgaagtg aacgcagcga aacaagcatt aaatggtaat
    30121 gacaatttgg caaatgcaaa acaacaagca aaacaacaat tggcgaactt aacacactta
    30181 aatgatgcac aaaaacaatc atttgaaagt caaattacac aagcgccact tgttacagat
    30241 gtcactacga ttaatcaaaa agcacaaacg ttagatcatg cgatggaatt attaagaaat
    30301 agtgttgcgg ataatcaaac gacattagcg tctgaagatt atcatgatgc aactgcgcaa
    30361 agacaaaatg actataacca agctgtaaca gctgctaata atatcattaa tcaaactaca
    30421 tcgcctacga tgaatccaga tgatgttaat ggtgcaacga cacaagtgaa taatacgaaa
    30481 gttgcattag atggtgatga aaaccttgca gcagctaaac aacaagcaaa caacagactt
    30541 gatcaattag atcatttgaa taatgcgcaa aagcaacagt tacaatcaca aattacgcaa
    30601 tcatctgata ttgctgcagt taatggtcac aaacaaacag cagaatcttt aaatactgcg
    30661 atgggtaact taattaatgc gattgcagat catcaagccg ttgaacaacg tggtaacttc
    30721 atcaatgctg atactgataa acaaactgct tataatacag cggtaaatga agcagcagca
    30781 atgattaaca aacaaactgg tcaaaatgcg aaccaaacag aagtagaaca agctattact
    30841 aaagttcaaa caacacttca agcgttaaat ggagaccata atttacaagt tgctaaaaca
    30901 aatgcgacgc aagcaattga tgctttaaca agcttaaatg atcctcaaaa aacagcatta
    30961 aaagaccaag ttacagctgc aactttagta actgcagttc atcaaattga acaaaatgcg
    31021 aatacgctta accaagcaat gcatggttta agacagagca ttcaagataa cgcagcaact
    31081 aaagcaaata gcaaatatat caacgaagat caaccagagc aacaaaacta tgatcaagct
    31141 gttcaagccg caaataatat tatcaatgaa caaactgcaa cattagataa taatgcgatt
    31201 aatcaagcag cgacaactgt gaatacaacg aaagcagcat tacatggtga tgtgaagtta
    31261 caaaatgata aagatcatgc taagcaaacg gttagtcaat tagcacatct aaacaatgca
    31321 caaaaacata tggaagatac gttaattgat agtgaaacaa ctagaacagc agttaagcaa
    31381 gatttgactg aagcacaagc attagatcaa cttatggatg cattacaaca aagtattgct
    31441 gacaaagatg caacacgtgc gagcagtgca tatgtcaatg cagaaccgaa taaaaaacaa
    31501 tcctatgatg aagcagttca aaatgctgag tctatcattg caggattaaa taatccaact
    31561 atcaataaag gtaatgtatc aagtgcgact caagcagtaa tatcatctaa aaatgcatta
    31621 gatggtgttg aacgattagc tcaagataag caaactgctg gaaattctct aaatcattta
    31681 gatcaattaa caccagctca acaacaagcg ctagaaaatc aaattaataa tgcaacaact
    31741 cgtggcgaag tagcacaaaa attaactgaa gcacaagcac ttaaccaagc aatggaagct
    31801 ttacgtaata gcattcaaga tcaacagcaa acggaagcgg gtagcaagtt tatcaatgaa
    31861 gataaaccac aaaaagatgc ttaccaagca gcagttcaaa atgcaaaaga tttaattaat
    31921 caaactaaca atccaacgct tgataaagca caagttgaac aattgacaca agctgttaac
    31981 caagctaaag ataacctaca cggtgatcaa aaacttgcag acgataaaca acatgcggtt
    32041 actgatttaa atcaattaaa tggtttgaat aatccgcaac gtcaagcact tgaaagccaa
    32101 ataaacaacg cagcaactcg tggcgaagta gcacaaaaat tagctgaagc aaaagcgctt
    32161 gatcaagcaa tgcaagcatt acgtaatagt attcaagatc aacaacaaac agaatctggt
    32221 agcaagttta tcaatgaaga taaaccgcaa aaagatgctt accaagcagc agttcaaaat
    32281 gcaaaagatt taattaacca aacaggtaat ccaacactcg acaaatcaca agtagaacaa
    32341 ttgacacaag cagtaacaac tgcaaaagat aatctacatg gtgatcaaaa acttgctcgt
    32401 gatcaacaac aagcagtaac aactgtaaat gcattgccaa acttaaatca tgcacaacaa
    32461 caagcattaa ctgatgctat aaatgcagcg cctacaagaa cagaggttgc acaacatgtt
    32521 caaactgcta ctgaacttga tcacgcgatg gaaacattga aaaataaagt tgatcaagtg
    32581 aatacagata aggctcaacc aaattacact gaagcgtcaa ctgataaaaa agaagcagta
    32641 gatcaagcgt tacaagctgc agaaagcatt acagatccaa ctaatggttc aaatgcgaat
    32701 aaagacgctg tagaccaagt attaactaag cttcaagaaa aagaaaatga gttaaatggt
    32761 aatgagagag tcgctgaagc taaaacacaa gcgaaacaaa ctattgacca attaacacat
    32821 ttaaatgctg atcaaattgc aactgctaaa caaaacattg atcaagcgac gaaacttcaa
    32881 ccaattgctg aattagtaga tcaagcaacg caattgaatc aatctatgga tcaattacaa
    32941 caagcagtta atgaacatgc taacgttgag caaactgtag attacacaca agcagattca
    33001 gataaacaaa atgcttataa acaagctatt gctgatgctg aaaatgtatt gaaacaaaat
    33061 gcgaataagc aacaagtgga tcaagcactt caaaatattt taaatgcaaa acaagcatta
    33121 aatggtgatg aacgtgtagc acttgctaaa acaaatggta aacatgacat cgaccaattg
    33181 aatgcattaa acaatgctca acaagatgga tttaaaggtc gcatcgatca atcaaacgat
    33241 ttaaatcaaa tccaacaaat tgtagatgag gctaaggcac ttaatcgtgc aatggatcaa
    33301 ttgtcacaag aaatcactga caatgaagga cgcacgaaag gtagcacgaa ctatgtcaat
    33361 gcagatacac aagtcaaaca agtatatgat gaaacggttg ataaagcgaa acaagcactt
    33421 gataaatcga ctggtcaaaa cttaactgca aaacaagtta tcaaattaaa tgatgcagtc
    33481 actgcagcta agaaagcatt aaatggtgaa gaaagactta ataatcgtaa agctgaagca
    33541 ttacaaagat tggatcaatt aacacatcta aacaatgctc aaagacaatt agcaatccaa
    33601 caaattaata atgctgaaac gctaaataaa gcatctcgag caattaatag agcaactaaa
    33661 ttagataatg caatgggtgc agtacaacaa tatattgacg aacagcacct tggtgttatc
    33721 agcagcacaa attacatcaa tgcagatgac aatttgaaag caaattatga taatgcaatt
    33781 gcgaatgcag cacatgagtt agataaagtg caaggtaatg caattgcaaa agctgaagca
    33841 gagcaattga aacaaaatat tatcgatgct caaaatgcat taaatggaga ccaaaacctt
    33901 gcaaatgcca aagataaagc aaatgcgttt gttaattcgt taaatggatt aaatcaacag
    33961 caacaagatc ttgcacataa agcaattaac aatgccgata ctgtatcaga tgtaacagat
    34021 attgttaata atcaaattga cttaaatgat gcaatggaaa cattgaaaca tttagttgac
    34081 aatgaaattc caaatgcaga gcaaactgtc aattaccaaa acgctgacga taatgctaaa
    34141 acaaacttcg atgatgccaa acgtctagca aatacattgc taaatagtga taacacaaat
    34201 gtgaatgata tcaatggcgc aatccaagca gtcaatgatg caatccataa tcttaatggt
    34261 gatcaacgac tacaagatgc taaagacaag gcaattcaat ctattaatca agctttagct
    34321 aataagctaa aagaaatcga agcttcaaat gcgacggatc aagacaagct tattgcgaaa
    34381 aataaagcag aagaattggc aaacagcatc atcaacaaca ttaataaagc aacaagtaat
    34441 caggctgtat ctcaagttca aacagcaggc aaccacgcga ttgaacaagt gcatgccaat
    34501 gaaataccaa aagcaaaaat tgatgccaat aaagacgttg ataagcaagt tcaagcatta
    34561 attgacgaaa ttgatcgaaa tccaaatcta acagataagg aaaaacaagc acttaaagat
    34621 cgtattaatc aaatacttca acaaggtcat aacggcatta acaatgcgat gactaaagaa
    34681 gaaattgaac aagccaaagc acaacttgcg caagcattac aagacatcaa agatttagtg
    34741 aaagctaaag aagatgcgaa acaagatgtt gataaacaag ttcaagcttt aattgacgaa
    34801 atcgatcaaa atccaaatct aacagataag gaaaaacaag cacttaaata tcgaattaat
    34861 caaatacttc aacaaggtca taacgacatt aacaatgcgc tgactaaaga agaaattgag
    34921 caggcaaaag cacaacttgc acaagcattg caagacatca aagatttagt gaaagctaaa
    34981 gaagatgcga aaaatgcaat aaaagcctta gctaatgcga agcgtgatca aatcaattca
    35041 aatccagatt taacacctga gcaaaaagca aaagcgctca aagaaattga cgaagctgaa
    35101 aaacgagcac tacaaaacgt tgagaatgct caaactatag atcaattaaa tcgaggatta
    35161 aacttaggtt tagatgacat tagaaataca catgtatggg aggttgatga acaacctgct
    35221 gtaaatgaaa tttttgaagc aacacctgag caaatcctag ttaatggtga actcattgta
    35281 catcgtgatg acatcattac agaacaagat attcttgcac acataaactt aattgatcag
    35341 ctttcagcag aagtcatcga tacaccatca actgcaacga tttctgatag cttaacagca
    35401 aaagttgaag ttacattgct tgatggatca aaagtgattg ttaatgttcc tgtaaaagtt
    35461 gtagaaaaag aattgtcagt agtcaaacaa caggcaattg aatcaatcga aaatgcggca
    35521 caacaaaaga ttaatgaaat caataatagt gtgacattaa cactggaaca aaaagaagct
    35581 gcaattgcag aagttaataa gcttaaacaa caagcaattg atcatgttaa caatgcacct
    35641 gatgttcatt cagttgaaga aattcaacaa caagaacaag cgcatattga acaatttaat
    35701 ccagaacaat ttacgattga acaagcaaaa tcaaatgcaa ttaaatcgat tgaagatgca
    35761 attcaacata tgattgatga aatcaaagct cgtactgatc taacagataa agagaagcaa
    35821 gaagctattg ctaagttaaa tcaattaaaa gaacaagcaa ttcaagcgat tcaacgtgcg
    35881 caaagcatcg atgaaataag tgagcaattg gaacaattta aagctcaaat gaaagcagct
    35941 aatccaacag caaaagaact agctaaacgc aagcaagaag ctattagtag aattaaagac
    36001 ttttcaaatg aaaaaataaa tagtattcga aatagtgaaa ttggcacagc tgatgaaaaa
    36061 caagcagcaa tgaatcaaat taacgaaatt gtgcttgaaa caattagaga tattaataat
    36121 gcgcatacat tacagcaagt tgaggctgca ttgaacaatg gtattgctcg aatttcagca
    36181 gtacaaattg taacatctga tcgtgctaaa caatcgtcaa gtactggaaa tgaatctaat
    36241 agccatttaa caattggtta tggaactgca aatcatccat ttaacagttc gactattgga
    36301 cataaaaaga aacttgatga agatgatgac attgatccac ttcatatgcg tcactttagt
    36361 aataatttcg gtaatgttat taaaaacgct attggtgtgg tgggtatctc tggtttacta
    36421 gctagtttct ggttcttcat tgccaaacgt cgtcgtaaag aagatgaaga ggaagaatta
    36481 gaaataagag ataataataa agattcaata aaagagactt tagacgatac aaaacattta
    36541 ccacttttat ttgcgaaacg tcgcagaaaa gaagatgaag aagatgttac tgttgaagaa
    36601 aaagattcgc taaataatgg cgagtcactc gataaagtta aacatacgcc gttcttctta
    36661 ccaaaacgtc gtcgtaaaga agatgaagaa gatgtggaag ttacaaatga aaacacagat
    36721 gaaaaagtgt tgaaagataa cgaacattca ccactcttat tcgcaaaacg acgcaaagat
    36781 aaagaggaag atgttgaaac aacaactagt attgaatcta aagatgagga cgttccttta
    36841 ttattggcta aaaagaaaaa tcaaaaagat aaccaatcca aagacaaaaa gtcagcatca
    36901 aaaaatactt ctaaaaaggt agcagctaaa aagaagaaaa agaaagctaa gaaaaataaa
    36961 aaataatttg tttctttgat aaatagagga gcaccgattg acatcacatc agtcggtgct
    37021 ccttttattt attcttttta attaatttat acaatgcctg ttgagcgtgt tgattcgctt
    37081 ctttgttttg ttctctcggt atccatttaa caaataataa atcaaaatct ttttcaaata
    37141 tttctatttg atcaaaataa ggtttgaaat ttgcgttttt cacataacca gcttcaatgc
    37201 tatctgcaat tagctttgag tctgtatata atagtgcgtt ttgaacattt aattcacgtg
    37261 catgttctag tgcataaata catgcagccc attctgcagt gtggttatcc atttcgccta
    37321 actcatgtgt atatgtataa tgctgctcat cttctttgat tacaatggca catgtactta
    37381 tgcctggatt tcctttcgtc gcagcatcaa aatttatttt cgccataata aacctacttt
    37441 ctattcaata cttagttaaa gttactatta ctgtaataca aaatatgttg ggtaatccat
    37501 taaaaaacac gcatcactta aataagtaac acgtgtttaa aatactcgct gattcaaaga
    37561 tgattttcta atacgtagta ctgtaatata cttcctaaaa aaatcatctt caggctggga
    37621 cataaatcaa tgttctatgc tctacga
//
LOCUS       DALAGD010000010        37497 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.010, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000010 DALAGD010000000
VERSION     DALAGD010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 37497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37497
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.010"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            59..823
                     /locus_tag="O2701_000364"
     CDS             59..823
                     /locus_tag="O2701_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCZ4292277.1"
                     /translation="MILKFDHIIHYIDQLDRFSFPGDVIKLHSGGYHHKYGTFNKLGY
                     INENYIELLDVENNEKLKKMAKTIEGGVAFATQIVQEKYEQGFKNICLRTNDIEAVKN
                     KLQSEQVEVVGPIQMERDTHKDGKVKWQLLYIMNQDDDEIKPPFFIQWEESDSMRTKK
                     LQKYFQKQFSIETVIVKSKNRSQTVSNWLKWFDMDIVEENDHYTDLILKNDDIYFRIE
                     DGKVSKYHSVIIKDAQATSPYSIFIRGAIYRFEPLV"
     gene            complement(815..1066)
                     /locus_tag="O2701_000365"
     CDS             complement(815..1066)
                     /locus_tag="O2701_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292278.1"
                     /translation="MRTIILSLFIIMNIVAIIMTLSQPLTVNYFSLRVILIFFTFILS
                     IFFILIKSSRLNNILTILSIVLVIIHMGILAHSTYVYLY"
     gene            1455..2720
                     /gene="femX"
                     /locus_tag="O2701_000366"
                     /gene_synonym="fmhB"
     CDS             1455..2720
                     /gene="femX"
                     /locus_tag="O2701_000366"
                     /gene_synonym="fmhB"
                     /EC_number="2.3.2.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase"
                     /protein_id="HCZ4292279.1"
                     /translation="MEKMHITNQEHDAFVKSHPNGDLLQLTKWAETKKLTGWYARRIA
                     VGRDGEVQGVAQLLFKKVPKLPYTLCYISRGFVVDYSNKEALNALLDSAKEIAKAEKA
                     YAIKIDPDVEVDKGTDALQNLKALGFKHKGFKEGLSKDYIQPRMTMITPIDKNDDELL
                     NSFERRNRSKVRLALKRGTTVERSDREGLKTFAELMKITGERDGFLTRDISYFENIYD
                     ALHEDGDAELFLVKLDPKENIAKVNQELNELHAEIAKWQQKMKTSEKQAKKAQNMIND
                     AQNKIAKNEDLKRDLEALEKEHPEGIYLSGALLMFAGSKSYYLYGASSNEFRDFLPNH
                     HMQYTMMKYAREHGATTYDFGGTDNDPDKDSEHYGLWAFKKVWGTYLSEKIGEFDYVL
                     NQPLYQLIEQVKPRLTKAKIKISRKLKRK"
     gene            2837..6004
                     /locus_tag="O2701_000367"
     CDS             2837..6004
                     /locus_tag="O2701_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HCZ4292280.1"
                     /translation="MIKKLLQFSLGNKFAIFLMVVLVVLGGVYASAKLKLELLPNVQN
                     PVISVTTTMPGATPQSTQDEISSKIDNQVRSLAYVKNVKTQSIQNASIVTVEYENNTD
                     MDKAEEQLKKEIDKIKFKDEVGQPELRRNSMDAFPVLAYSFSNKENDLKKVTKVLNEQ
                     LIPKLQTVDGVQNAQLNGQTNREITLKFKQNELEKYGLTADDVENYLKTATRTTPLGL
                     FQFGDKDKSIVVDGQYQSVDAFKNINIPLTLAGGQGQSQSQSDNKQNSAMSDVNQASP
                     QQNSKASAPNNISGMPTAKLGDLADITVGDVRTSISKTNGKDAVNLQITKAQDANTVQ
                     VAKDVQRKIDTFVDENKDFNVTKTMDTAKPVEKSLYTMVEKASLGTIVAIIVILLFLR
                     NIRTTAISIISIPLSLLMALIALKLSDVSLNILTLGALTVAIGRVIDDSIVVVENIYR
                     RLTDSEEQLKGENLIISATTEVFKPIMSSTLVTIIVFLPLVFVSGSVGEMFRPFALAI
                     AFSLLASLLVSITLVPALAATLFKKGVKRRNKQHQEGLGVVSTTYKKVLHWSLNHKWI
                     VIILSTLILVATIVFGGPRLGTSFISAGDDKFLAITYTPKPGETEQAVLNHAKDVEKY
                     LKQKKHVKTIQYSVGGSSPVDPTGSTNSMAIMVEYDNDTPNFDVEADKVIKHADGFKH
                     PGEWKNQDLGTGAGNKSVEVTVKGPSMDAIKSTVKDIEQKMKQVKGLANVKSDLSQTY
                     DQYEIKVDQNKAAENGISASQLAMHLNENLPEKTVTTVKENGKTVDVKVKQNKQTDWS
                     EDKLNNITLKKPTGGTIKLGDIATLVKTTTPSKLTQEQGDYATTVSAKVTNKDVGGTT
                     RQVMSKINNLDKPNNVKVNIGGASDDINNAMTQLAFAMLAAIIIVYLILVITFKGGLA
                     PFTILFSLPFTVIGVIIALLITGETISVPSLIGMLMLIGIVVTNAIVLIDRVINNEQQ
                     GMEMKEALIEAGGTRIRPILMTAIATIGALVPLLFGQDSSILISKGLAATVIGGLISS
                     TLLTLVVVPVIYEILFTLKKRFTKR"
     gene            complement(6191..6508)
                     /gene="mspA"
                     /locus_tag="O2701_000368"
     CDS             complement(6191..6508)
                     /gene="mspA"
                     /locus_tag="O2701_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane stabilizing protein MspA"
                     /protein_id="HCZ4292281.1"
                     /translation="MQFYLILLAILYLIVSFISIFKMEVVFTRILRIIMGVLLLFVLA
                     LTTMSFPKENWWVFIVLLLLVGNVEVTGFKMLKKDLKGVNILNLMSLFIFVIYFILTI
                     VLF"
     gene            complement(6753..6917)
                     /locus_tag="O2701_000369"
     CDS             complement(6753..6917)
                     /locus_tag="O2701_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292282.1"
                     /translation="MSLENQLAELKYDYVRLQGDIEKRESLNLDTSALVRQLKDIENE
                     IRNVRAQMQD"
     gene            7050..7958
                     /locus_tag="O2701_000370"
     CDS             7050..7958
                     /locus_tag="O2701_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HCZ4292283.1"
                     /translation="MTEQFTMIILLIALGYFLKRINFIKATDSQVIATLVLNVTLPSL
                     VIVNLNSAELKLSFSILPILMIIYGIVAKMIAVWFFRKYDNHMRGSVGMMTGAMNIGL
                     FAYPLIEVIWPKTGLIYFVMADIGGAFVMFGITYFVGSYFSEGSDQFDFKYLGKKLLQ
                     SVPLVTYIVMFILNISHIHLPHVAIDFFSILSHANMPLSMILLGVMLNFTLERKYIPA
                     TIKYLCLHYGLALVAGLLVYVLLPVSDDMIKTTLLVTWMFPVGVAIISYGIQFKYRTL
                     TFIGMTTNLTIIISIIILYVYQAVFV"
     gene            8129..8260
                     /locus_tag="O2701_000371"
     CDS             8129..8260
                     /locus_tag="O2701_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292284.1"
                     /translation="MYGAYLIYEYHQDFKSIANFKVLKRMINVMMCYCVLFLEPRIS"
     gene            complement(8377..9240)
                     /locus_tag="O2701_000372"
     CDS             complement(8377..9240)
                     /locus_tag="O2701_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein GlcU"
                     /protein_id="HCZ4292285.1"
                     /translation="MQFLDFLIALLPALFWGSVVLINVFVGGGPYNQIRGTTLGALIV
                     GLGLLITGFAKFNNPTVIIVGLISGALWAFGQANQLKSISLIGVSNTMPVSTGMQLVG
                     TTLFSVIFLGEWSSMTQIIFGLIAMILLVTGVALTSLKAKNERQSDNPEFKKAMGILI
                     VSTVGYVGFVVLGDIFGVGGTDALFFQSVGMAIGGFILSMNHKTSLKSTALNLLPGVI
                     WGIGNLFMFYSQPKVGVATSFSLSQLLVIVSTLGGIFILGERKDRRQMTGIWAGIIII
                     VIAAIILGNLK"
     gene            complement(9421..10308)
                     /locus_tag="O2701_000373"
     CDS             complement(9421..10308)
                     /locus_tag="O2701_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4292286.1"
                     /translation="MSIVQLYDITQIKSFIEHSNYESASYLYKLPQQYNEIDVLITDA
                     IESPGVFSIKENDSIKAIILSFAYDKNKFKVIGPFVADNYVLSVDTFETLFKAMTSNQ
                     PDDAVFNFSFEEGIQQYKPLMKVIQASYNFTDYYIEARTRLEEDMHQPNIIPYHKGFY
                     RAFSKLHTTTFKYQAQSPQDIIDSLDDHHHLFLFVSEGLLKGYLYLEIDSQQSIAEIK
                     YFSSHVDYRLKGIAFELLAYALQYAFDNFDIRKVYFKIRNKNNKLIERFNGLGFHINY
                     EYIKFKFESRNVKDQTIPE"
     gene            10374..10469
                     /locus_tag="O2701_000374"
     CDS             10374..10469
                     /locus_tag="O2701_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292287.1"
                     /translation="MILTCYNTFNTSFAIIEFKSKISGIDEARSK"
     gene            10495..12630
                     /locus_tag="O2701_000375"
     CDS             10495..12630
                     /locus_tag="O2701_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCZ4292288.1"
                     /translation="MKSLILAEKPSVARDIADALQINQKRNGYFENNQYIVTWALGHL
                     VTNATPEQYDKNLKEWRLEDLPIIPKYMKTVVIGKTSKQFKTVKALILDNKVKDIIIA
                     TDAGREGELVARLILDKVGNKKPIRRLWISSVTKKAIQQGFKNLKDGRQYNDLYYAAL
                     ARSEADWIVGINATRALTTKYDAQLSLGRVQTPTIQLVNTRQQEINQFKPQQYFTLSL
                     TVKGFDFQLESNQRYTNKETLEQMVNNLKNVDGKIKSVATKHKKSYPQSLYNLTDLQQ
                     DMYRRYKIGPKETLNTLQSLYERHKVVTYPRTDSNYLTTDMVDTMKERIQATMATTYK
                     DQARPLMSKTFSSKMSIFNNQKVSDHHAIIPTEVRPVMSDLSNRELKLYDMIVERFLE
                     ALMPPHEYDAITVTLEVAGHTFVLKENVTTVLGFKSIRQGESITEMQQPFSEGDEVKI
                     SKTNIREHETTPPEYFNEGSLLKAMENPQNFIQLKDKKYAQTLKQTGGIGTVATRADI
                     IDKLFNMNAIESRDGKIKVTSKGKQILELAPEELTSPLLTAQWEEKLLLIERGKYQAK
                     TFINEMKDFTKDVVNGIKNSDRKYKHDNLTTTECPTCGKFMIKVKTKNGQMLVCQDPS
                     CKTKKNVQRKTNARCPNCKKKLTLFGKGKEAVYRCVCGHSETQAHMDQRMKSKSSGKV
                     SRKEMKKYMNKNEGLDNNPFKDALKNLNL"
     gene            12743..14077
                     /locus_tag="O2701_000376"
     CDS             12743..14077
                     /locus_tag="O2701_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCZ4292289.1"
                     /translation="MKNYFQFDKYGTNFKREILGGITTFLSMAYILAVNPQVLSLAGV
                     KGVSEDMKMDQGAIFVATALAAFVGSLFMGLIAKYPIALAPGMGLNAFFAFTVVLTMG
                     IPWQVGLTGVLFSGIFFAILTVTGFREVIINAIPYQMKMAVSAGIGLFITFVGLQSSG
                     IIVKNESTLVTLGHLTDPPVLLAIFGIVITVILYAIKLPGSIFIGMIITAIVGMFTGL
                     IQMPSGIVGKIPSIEPTFGAAFEAFKDPSQLLTIQFLIVILTFLFIDFFDTAGTLVAV
                     ATQAGIMKDNKLPRAGRALFSDSLATIVGSVFGTTTTTSYIESTSGVAVGARTGFASI
                     VTGCCFLLALFFSPLIAVVTSAVTTPALVVVGVLMAANFAEINWKSFEVAVPAFITII
                     MMPLSYSIATGIACGFIFYPITMLISKKHKEVHPIMYVLMVLFILYFIFVHG"
     gene            complement(14269..14658)
                     /locus_tag="O2701_000377"
     CDS             complement(14269..14658)
                     /locus_tag="O2701_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292290.1"
                     /translation="MKNGPKLSLALIGIFLILCEFFYGIPFLGATFILSFGWQPLLFN
                     ALLYLILTIILLVNRQNAIRPIAIIPIFGIVGSFLAIIPFLGILIHWILFFLMILFVL
                     VVLSAPTYIPNKNARVIYTQYKDGHRE"
     gene            15015..15323
                     /gene="rpsJ"
                     /locus_tag="O2701_000378"
     CDS             15015..15323
                     /gene="rpsJ"
                     /locus_tag="O2701_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCZ4292291.1"
                     /translation="MAKQKIRIRLKAYDHRVIDQSAEKIVETAKRSGADVSGPIPLPT
                     EKSVYTIIRAVHKYKDSREQFEQRTHKRLIDIVNPTPKTVDALMGLNLPSGVDIEIKL
                     "
     gene            15351..16013
                     /gene="rplC"
                     /locus_tag="O2701_000379"
     CDS             15351..16013
                     /gene="rplC"
                     /locus_tag="O2701_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCZ4292292.1"
                     /translation="MTKGILGRKIGMTQVFGENGELIPVTVVEAKENVVLQKKTVEVD
                     GYNAIQVGFEDKKAYKKDAKSNKYANKPAEGHAKKADAAPKRFIREFRNVDVDAYEVG
                     QEVSVDTFVAGDVIDVTGVSKGKGFQGAIKRHGQSRGPMSHGSHFHRAPGSVGMASDA
                     SRVFKGQKMPGRMGGNTVTVQNLEVVQVDTENKVILVKGNVPGPKKGLVEIRTSIKKG
                     NK"
     gene            16040..16663
                     /gene="rplD"
                     /locus_tag="O2701_000380"
     CDS             16040..16663
                     /gene="rplD"
                     /locus_tag="O2701_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCZ4292293.1"
                     /translation="MANYDVLKLDGTKSGSIELSDAVFGIEPNNSVLFEAINLQRASL
                     RQGTHAVKNRSAVSGGGRKPWKQKGTGRARQGTIRAPQWRGGGIVFGPTPRSYAYKMP
                     KKMRRLALRSALSFKAQENGLTVVDAFNFEAPKTKEFKNVLSTLEQPKKVLVVTENED
                     VNVELSARNIPGVQVTTAQGLNVLDITNADSLVITEAAAKKVEEVLG"
     gene            16663..16938
                     /gene="rplW"
                     /locus_tag="O2701_000381"
     CDS             16663..16938
                     /gene="rplW"
                     /locus_tag="O2701_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002506966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCZ4292294.1"
                     /translation="MEARDILKRPVITEKSSEAMAEDKYTFDVDTRVNKTQVKMAVEE
                     IFNVKVASVNIMNYKPKKKRMGRYQGYTNKRRKAIVTLKEGSIDLFN"
     gene            16971..17804
                     /gene="rplB"
                     /locus_tag="O2701_000382"
     CDS             16971..17804
                     /gene="rplB"
                     /locus_tag="O2701_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCZ4292295.1"
                     /translation="MAIKKYKPITNGRRNMTSLDFAEITKTTPEKSLLKPLPKKAGRN
                     NQGKLTVRHHGGGHKRQYRVIDFKRNKDGINAKVDSIQYDPNRSANIALVVYADGEKR
                     YIIAPKGLEVGQIVESGAEADIKVGNALPLQNIPVGTVVHNIELKPGKGGQIARSAGA
                     SAQVLGKEGKYVLIRLRSGEVRMILSTCRATIGQVGNLQHELVNVGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGRAPIGRPSPMSPWGKPTLGKKTRRGKKSSDKLIVRGRKK
                     K"
     gene            17871..18149
                     /gene="rpsS"
                     /locus_tag="O2701_000383"
     CDS             17871..18149
                     /gene="rpsS"
                     /locus_tag="O2701_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCZ4292296.1"
                     /translation="MARSIKKGPFVDEHLMKKVEAQEGSEKKQVIKTWSRRSTIFPNF
                     IGHTFAVYDGRKHVPVYVTEDMVGHKLGEFAPTRTFKGHVADDKKTRR"
     gene            18178..18531
                     /gene="rplV"
                     /locus_tag="O2701_000384"
     CDS             18178..18531
                     /gene="rplV"
                     /locus_tag="O2701_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCZ4292297.1"
                     /translation="MEAKAVARTIRIAPRKVRLVLDLIRGKNAAEAIAILKLTNKASS
                     PVIEKVLMSALANAEHNYDMNTDELVVKEAYANEGPTLKRFRPRAQGRASAINKRTSH
                     ITIVVSDGKEEAKEA"
     gene            18555..19208
                     /gene="rpsC"
                     /locus_tag="O2701_000385"
     CDS             18555..19208
                     /gene="rpsC"
                     /locus_tag="O2701_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCZ4292298.1"
                     /translation="MGQKINPIGLRVGIIRDWEAKWYAEKDFASLLHEDLKIRKFIDN
                     ELKEASVSHVEIERAANRINIAIHTGKPGMVIGKGGSEIEKLRNKLNALTDKKVHINV
                     IEIKKVDLDARLVAENIARQLENRASFRRVQKQAITRAMKLGAKGIKTQVSGRLGGAD
                     IARAEQYSEGTVPLHTLRADIDYAHAEADTTYGKLGVKVWIYRGEVLPTKNTSGGGK"
     gene            19211..19645
                     /gene="rplP"
                     /locus_tag="O2701_000386"
     CDS             19211..19645
                     /gene="rplP"
                     /locus_tag="O2701_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCZ4292299.1"
                     /translation="MLLPKRVKYRRQHRPKTTGRSKGGNYVTFGEFGLQATTTSWITS
                     RQIESARIAMTRYMKRGGKVWIKIFPHTPYTKKPLEVRMGAGKGAVEGWIAVVKPGRI
                     LFEVAGVSEEVAREALRLASHKLPVKTKFVKREELGGETNES"
     gene            19635..19844
                     /gene="rpmC"
                     /locus_tag="O2701_000387"
     CDS             19635..19844
                     /gene="rpmC"
                     /locus_tag="O2701_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002509269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCZ4292300.1"
                     /translation="MKAKEIRDLTTSEIEEQIKSSKEELFNLRFQLATGQLEETARIR
                     TVRKTIARLKTVAREREIEQSKANQ"
     gene            19868..20131
                     /gene="rpsQ"
                     /locus_tag="O2701_000388"
     CDS             19868..20131
                     /gene="rpsQ"
                     /locus_tag="O2701_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCZ4292301.1"
                     /translation="MSERNDRKVYVGKVVSDKMDKTITVLVETYKTHKLYGKRVKYSK
                     KYKTHDENNSAKLGDIVKIQETRPLSATKRFRLVEIVEESVII"
     gene            20163..20531
                     /gene="rplN"
                     /locus_tag="O2701_000389"
     CDS             20163..20531
                     /gene="rplN"
                     /locus_tag="O2701_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCZ4292302.1"
                     /translation="MIQQETRLKVADNSGAREVLTIKVLGGSGRKTANIGDVIVCTVK
                     NATPGGVVKKGDVVKAVIVRTKSGVRRNDGSYIKFDENACVIIRDDKGPRGTRIFGPV
                     ARELREGNFMKIVSLAPEVL"
     gene            20567..20884
                     /gene="rplX"
                     /locus_tag="O2701_000390"
     CDS             20567..20884
                     /gene="rplX"
                     /locus_tag="O2701_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCZ4292303.1"
                     /translation="MHIKKGDNVKVIAGKDKGKEGKVIATLPKKDRVVVEGVNIMKKH
                     QKPTQLNPEGGILETEAAIHVSNVQLLDPKTNEPTRVGYKFVDGKKVRIAKKSGEEIK
                     SNN"
     gene            20911..21450
                     /gene="rplE"
                     /locus_tag="O2701_000391"
     CDS             20911..21450
                     /gene="rplE"
                     /locus_tag="O2701_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCZ4292304.1"
                     /translation="MNRLKEKFNTEVTENLMKKFNYSSVMEVPKIDKIVVNMGVGDAV
                     QNSKVLDNAVEELELITGQKPLVTKAKKSIATFRLREGMPIGAKVTLRGERMYEFLDK
                     LISVSLPRVRDFQGVSKKAFDGRGNYTLGVKEQLIFPEIDYDKVSKVRGMDIVIVTTA
                     NTDEEARELLANFGMPFRK"
     gene            21473..21658
                     /locus_tag="O2701_000392"
     CDS             21473..21658
                     /locus_tag="O2701_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HCZ4292305.1"
                     /translation="MAKTSMVAKQQKKQKYAVREYTRCERCGRPHSVYRKFKLCRICF
                     RELAYKGQIPGVRKASW"
     gene            21690..22088
                     /gene="rpsH"
                     /locus_tag="O2701_000393"
     CDS             21690..22088
                     /gene="rpsH"
                     /locus_tag="O2701_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCZ4292306.1"
                     /translation="MTMTDPIADMLTRVRNANMVRHEKLELPASNIKKEIAEILKSEG
                     FIKNVEYVEDDKQGVLRLFLKYGQNDERVITGLKRISKPGLRVYAKASEMPKVLNGLG
                     IALVSTSEGVITDKEARKRNVGGEIIAYVW"
     gene            22113..22649
                     /gene="rplF"
                     /locus_tag="O2701_000394"
     CDS             22113..22649
                     /gene="rplF"
                     /locus_tag="O2701_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCZ4292307.1"
                     /translation="MSRVGKKIIDIPSDVTVTFDGNHVTVKGPKGELSRTLNERMTFK
                     QEENTIEVVRPSDSKEDRTNHGTTRALLNNMVQGVSQGYVKVLELVGVGYRAQMQGKD
                     LILNVGYSHPVEIKAEENITFSVEKNTVVKVEGISKEQVGALASNIRSVRPPEPYKGK
                     GIRYQGEYVRRKEGKTGK"
     gene            22680..23039
                     /gene="rplR"
                     /locus_tag="O2701_000395"
     CDS             22680..23039
                     /gene="rplR"
                     /locus_tag="O2701_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCZ4292308.1"
                     /translation="MISKIDKNKVRLKRHARVRTNLSGTAEKPRLNVYRSNKHIYAQI
                     IDDNKGVTLAQASSKDSDIATTATKVELATKVGEAIAKKAADKGIKEIVFDRGGYLYH
                     GRVKALAEAARESGLEF"
     gene            23060..23560
                     /gene="rpsE"
                     /locus_tag="O2701_000396"
     CDS             23060..23560
                     /gene="rpsE"
                     /locus_tag="O2701_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCZ4292309.1"
                     /translation="MARREEETKEFEERVVTINRVAKVVKGGRRFRFTALVVVGDKNG
                     RVGFGTGKAQEVPEAIKKAVEAAKKDLVVVPRVEGTTPHTITGRYGSGSVFMKPAAPG
                     TGVIAGGPVRAVLELAGITDILSKSLGSNTPINMVRATIDGLQNLKNAEDVAKLRGKT
                     VEELYN"
     gene            23577..23756
                     /gene="rpmD"
                     /locus_tag="O2701_000397"
     CDS             23577..23756
                     /gene="rpmD"
                     /locus_tag="O2701_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCZ4292310.1"
                     /translation="MAKLQITLTRSVIGRPETQRKTVEALGLKKTNSSVVVEDNPAIR
                     GQINKVKHLVTVEEK"
     gene            23773..24213
                     /gene="rplO"
                     /locus_tag="O2701_000398"
     CDS             23773..24213
                     /gene="rplO"
                     /locus_tag="O2701_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCZ4292311.1"
                     /translation="MKLHELKPAEGSRKERNRVGRGVATGNGKTSGRGHKGQKARSGG
                     GVRPGFEGGQLPLFRRLPKRGFTNINRKEYAIVNLDQLNKFEDGTEVTPALLVESGVV
                     KNEKSGIKILGNGSLDKKLTVKAHKFSASAAEAIDAKGGAHEVI"
     gene            24213..25505
                     /gene="secY"
                     /locus_tag="O2701_000399"
     CDS             24213..25505
                     /gene="secY"
                     /locus_tag="O2701_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCZ4292312.1"
                     /translation="MIQTLVNFFRTKEVRNKIFFTLAMLVIFKIGTYIPAPGVNPAAF
                     DNPQGSQGATELLNTFGGGALKRFSIFAMGIVPYITASIVMQLLQMDIVPKFSEWAKQ
                     GEVGRRKLNNVTRYLAISLAFIQSIGMAFQFNNYLKGALIINQSIMSYLLIALVLTAG
                     TAFLIWLGDQITQFGVGNGISIIIFAGILSTLPASLIQFGQTAFVGQEDTSLAWLKVL
                     GLLVSLILLTVGAIYVLEAVRKIPIQYAKKQTAQRLGSQATYLPLKVNSAGVIPVIFA
                     MAFFLLPRTLTLFYPDKEWAQNIANAANPSSNVGMVVYIVLIILFTYFYAFVQVNPEK
                     MADNLKKQGSYVPGIRPGEQTKKYITKVLYRLTFVGSIFLAVISILPILATKFMGLPQ
                     SIQIGGTSLLIVIGVAIETMKSLEAQVSQKEYKGFGGR"
     gene            25522..26169
                     /locus_tag="O2701_000400"
     CDS             25522..26169
                     /locus_tag="O2701_000400"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCZ4292313.1"
                     /translation="MNIILMGLPGAGKGTQASEIVKKFPIPHISTGDMFRKAIKEETE
                     LGKEAKSYMDRGELVPDEVTVGIVKERISEDDAKKGFLLDGFPRTIEQAEALNNIMSE
                     LDRNIDAVINIEVPEEELMNRLTGRRICESCGTTYHLVFNPPKVEGICDIDGGKLYQR
                     EDDNPETVANRLSVNIKQSKPILDFYDQKGVLKNIDGSKDISDVTKDVIDILDHL"
     gene            26362..26580
                     /gene="infA"
                     /locus_tag="O2701_000401"
     CDS             26362..26580
                     /gene="infA"
                     /locus_tag="O2701_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCZ4292314.1"
                     /translation="MAKQDVIELEGTVLDTLPNAMFKVELENGHEILAHVSGKIRMNY
                     IRILPGDKVTVEMSPYDLTRGRITYRYK"
     gene            26612..26725
                     /gene="rpmJ"
                     /locus_tag="O2701_000402"
     CDS             26612..26725
                     /gene="rpmJ"
                     /locus_tag="O2701_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCZ4292315.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQRQG"
     gene            26748..27113
                     /gene="rpsM"
                     /locus_tag="O2701_000403"
     CDS             26748..27113
                     /gene="rpsM"
                     /locus_tag="O2701_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCZ4292316.1"
                     /translation="MARIAGVDIPREKRVVISLTYIYGIGTSTAQKILEEANVSADTR
                     VKDLTDDELGRIREVVDGYKVEGDLRRETNLNIKRLMEISSYRGIRHRRGLPVRGQKT
                     KNNARTRKGPVKTVANKKK"
     gene            27137..27526
                     /gene="rpsK"
                     /locus_tag="O2701_000404"
     CDS             27137..27526
                     /gene="rpsK"
                     /locus_tag="O2701_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCZ4292317.1"
                     /translation="MARKQVSRKRRVKKNIENGVAHIRSTFNNTIVTITDEFGNALSW
                     SSAGALGFKGSKKSTPFAAQMASETASKSAMEHGLKTVEVTVKGPGPGRESAIRALQS
                     AGLEVTAIRDVTPVPHNGCRPPKRRRV"
     gene            27601..28545
                     /locus_tag="O2701_000405"
     CDS             27601..28545
                     /locus_tag="O2701_000405"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCZ4292318.1"
                     /translation="MIEIEKPRIETIEISEDAKFGKFVVEPLERGYGTTLGNSLRRIL
                     LSSLPGAAVKYIEIEGVLHEFSAVDNVVEDVSTIIMNIKQLALKIYSEEDKTLEIDVR
                     DEGEVTASDITHDSDVEILNPELKIATVSKGGHLKIRLVANKGRGYALAEQNNTSDLP
                     IGVIPVDSLYSPVERVNYTVENTRVGQSSDFDKLTLDVWTNGSITPQESVSLAAKIMT
                     EHLNIFVGLTDEAQNAEIMIEKEEDQKEKVLEMSIEELDLSVRSYNCLKRAGINSVQE
                     LADKSEADMMKVRNLGRKSLEEVKYKLEDLGLGLRKED"
     gene            28562..28930
                     /gene="rplQ"
                     /locus_tag="O2701_000406"
     CDS             28562..28930
                     /gene="rplQ"
                     /locus_tag="O2701_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002450507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCZ4292319.1"
                     /translation="MGYRKLGRTSDQRKAMLRDLATSLIISERIETTEARAKEVRSVV
                     EKLITLGKKGDLASRRNAAKTLRNVEILNEDETTQTALQKLFGEIAERYTERQGGYTR
                     ILKQGPRRGDGAESVIIELV"
     gene            29468..30277
                     /locus_tag="O2701_000407"
     CDS             29468..30277
                     /locus_tag="O2701_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HCZ4292320.1"
                     /translation="MEDKNSVIVFKNVSFQYQSDASFTLKDVSFNIPKGQWTSIVGHN
                     GSGKSTIAKLMIGIEKVKSGEIFYNNQAITDDNFEKLRKDIGIVFQNPDNQFVGSIVK
                     YDVAFGLENHAVPYDEMHRRVSEALKQVDMLERADYEPNALSGGQKQRVAIASVLALN
                     PSVIILDEATSMLDPDARQNLLDLVRKVKSEHNITIISITHDLSEAMEADHVIVMNKG
                     TVYKEGTATEIFDNAEELTRIGLDLPFPIKINQMLGHQTSFLTYEGLVDQL"
     gene            30274..31134
                     /locus_tag="O2701_000408"
     CDS             30274..31134
                     /locus_tag="O2701_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HCZ4292321.1"
                     /translation="MTIRFDNVSYTYQKGTPYQHQAIHDVNTEFEQGKYYAIVGQTGS
                     GKSTLIQNINALLKPTTGTVTVDDITITHKTKDKYIRPVRKRIGMVFQFPESQLFEDT
                     VEREMIFGPKNFKMNLDEAKNYAHRLLMDLGFSRDVMSQSPFQMSGGQMRKIAIVSIL
                     AMNPDIIVVDEPTAGLDPQSKRQVMRLLKSLQTDENKAIILISHDMNEVARYADEVIV
                     MKEGSIVSQTSPKELFKDKKKLADWHIGLPEIVQLQYDFEQKYQTKLKDIALTEEAFV
                     SLYKEWQHEK"
     gene            31124..31930
                     /locus_tag="O2701_000409"
     CDS             31124..31930
                     /locus_tag="O2701_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCZ4292322.1"
                     /translation="MKNKLIIGRYLPINSFVHHLDPRAKLMFVFLFIILIFFCHSPLT
                     YLWVFALILFIMRLAKIQLWFLIKGLTPIFFFLIFTLMMHIFLTKGGYVLVEWHGITI
                     ETNGILEGLYISLRLIGIVMIATIMTLSTSPIDLTDAFERLLAPLKMFKLPVHQLSMI
                     MSIALRFIPTLMDELDKIILAQKSRGSEISSGNIATRIKSFIPLLVPLFISAFQRAEE
                     LAVAMEVRGYDANVKRTSYRQLKWQLRDTISLTMIIPIAIILFVLKYSGV"
     gene            31935..32738
                     /gene="truA"
                     /locus_tag="O2701_000410"
     CDS             31935..32738
                     /gene="truA"
                     /locus_tag="O2701_000410"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCZ4292323.1"
                     /translation="MRILVEIAYQGNNFLGFQIQQNGRTVQQQFEKLLQRMHKRHVRI
                     HPSSRTDRGVHAIQQYFHFDTELNIPMSQWQYAMNRTLPDDIYVNNVVTVDDDFHCRY
                     DCVGKRYRYKVYQAQHRDPFQSGLKTFIPETLDLGKMNRAAQQFIGTHDFTGFCSQKT
                     EVESKVRTLYQSEIVKTDDGFDYIVTGSGFLYNMVRVLVAFLIEVGKGRHEVSDVPKL
                     LESKNRKNVPFTAPAEGLYLEKIYLDENELLKDFGNDIKIHRKKSLQND"
     gene            32978..33415
                     /gene="rplM"
                     /locus_tag="O2701_000411"
     CDS             32978..33415
                     /gene="rplM"
                     /locus_tag="O2701_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCZ4292324.1"
                     /translation="MRQTFMANESNIERKWYVIDAEGQTLGRLSSEVASILRGKNKVT
                     YTPHVDTGDYVIVINASKIEFTGNKETDKVYYRHSNHPGGIKSITAGELRRTNPERLI
                     ENSIKGMLPSTRLGEKQGKKLFVYGGAEHPHAAQQPENYELRG"
     gene            33429..33827
                     /gene="rpsI"
                     /locus_tag="O2701_000412"
     CDS             33429..33827
                     /gene="rpsI"
                     /locus_tag="O2701_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCZ4292325.1"
                     /translation="MTLAQVEYRGTGRRKNSVARVRLVPGEGNITVNNRDVREYLPFE
                     SLILDLNQPFDVTETKGNYDVLVNVHGGGFTGQAQAIRHGIARALLEADPEYRGSLKR
                     AGLLTRDPRMKERKKPGLKAARRSPQFSKR"
     gene            34372..35469
                     /locus_tag="O2701_000413"
     CDS             34372..35469
                     /locus_tag="O2701_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCZ4292326.1"
                     /translation="MRGDQRTQGEKSRKEGGKIFGSGSRAPIAISILVKDGSYNHDIY
                     YNDIGEYLTREQKLDTLMKHQSIVNLKSLNVLPDKNNDWINQRDINYENYLPMYDSKD
                     IENSIYLDQFNGVNSARDNWVTNFSNEKALVNAKLLVDNYNSEIDRLIDILDSRERIN
                     LVNKDETFISWTRGLTQKFSKGKNISINPERIVKFMHRPFTKKWIVYDKNIMEMPSRY
                     YNIMENTGQVIYIQGQGMNKEFSAMITDILPNFQFIGNGKGFATYKGKDSLRLVDNIS
                     NSFKKKINLNSEEIVYYIYAILHHKYYVNKYSSDLSKGFPRIPILKDVYGFVEIGREL
                     VELHLNYEKQLNWDGVEIIYNNMNPNYKVEK"
     gene            35487..35759
                     /locus_tag="O2701_000414"
     CDS             35487..35759
                     /locus_tag="O2701_000414"
                     /inference="COORDINATES: protein motif:HMM:NF037202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCZ4292327.1"
                     /translation="MLDTVIYNTDITVTNIPNKVYNYIVNGKPAIEWIIDQYNVSIDK
                     KSGILDDPNEFSEDPNYILNLLLSVITVSMKTLGLIDKLPDFEVLD"
     gene            35820..36563
                     /locus_tag="O2701_000415"
     CDS             35820..36563
                     /locus_tag="O2701_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292328.1"
                     /translation="MDLLQIEKKGNLTKTVQLMHDEKGSASGESKVNMEFGKQSAVKQ
                     IFDSLLGMSASASASAGINGNITGERVAKTILENTLIYDFLDTVEFRKKKTIIDISEG
                     FKLNIQQDTMTYFATITPIASMFEGSKHLDEEELSFDVSKMNEAIRSIKGYFELIGTR
                     GNENRVFRFNIESFKNNYRIQDLKFMNLTLYSVLVGEISKENLNYDKEFDLNQGAKPS
                     GIGKFKGIRSEATKEDKYQVYDVILAGVK"
     gene            36564..>37497
                     /locus_tag="O2701_000416"
     CDS             36564..>37497
                     /locus_tag="O2701_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCZ4292329.1"
                     /translation="MEIHVYTGPLCGFNEYINSEVKEYHRFMELIREYNIIVRASDTF
                     GTHDVDGIFDTEIENLVIFSNDFASVTTHVITNFINIVTLGRNIKKIYIQNPPKRVFQ
                     SLNSFHDSEIIEINYEYTTLKKDDVINFYKEQMAGSKIIGQTDAKKQMSIDLYKHTSL
                     ENNKPTVMLYYGPSGVGKTELAKEIAKKYKGNITRIQFSMMQNDESFNYIFGDEHAKV
                     SLSKDLVDRETNVVLIDEFDKVLPIYYNVFYQMFDEGIIEDANYKVDVSNCIFILTSN
                     FNSIEEAIVNIGEPVFSRIDSCIKFLYLTQEEKQK"
BASE COUNT        13723 a         5584 c         7080 g        11110 t
ORIGIN      
        1 caaaaataat tatactttgt taaaataagc gtaattacat gtaaataggg ggatactaat
       61 gatattgaaa tttgatcaca tcattcatta tatagatcag ttagatcggt ttagttttcc
      121 aggagatgtt ataaaattac attcaggtgg gtatcatcat aaatatggaa cattcaataa
      181 attaggttat atcaatgaaa attatattga gctactagat gtagaaaata atgaaaagtt
      241 gaaaaagatg gcaaaaacga tagaaggcgg agtcgctttt gctactcaaa ttgttcaaga
      301 gaagtatgag caaggcttta aaaatatttg tttgcgtaca aatgatatag aggcagttaa
      361 aaataaacta caaagtgagc aggttgaagt agtagggccg attcaaatgg aaagagatac
      421 acataaagat ggtaaggtaa agtggcaatt gctttatata atgaatcagg atgatgatga
      481 aattaagcca ccatttttta ttcaatggga agaaagtgat tccatgcgta ctaaaaaatt
      541 gcaaaaatat tttcaaaaac aattttcaat tgaaactgtt attgtgaaaa gtaaaaaccg
      601 atcacaaaca gtatcgaatt ggttgaaatg gtttgatatg gacattgtag aagagaatga
      661 ccattacaca gatttgattt taaaaaatga tgatatttat tttagaattg aagatggtaa
      721 agtttcaaaa tatcattcgg ttatcataaa agacgcacaa gcaacttcac catattcaat
      781 ttttatcaga ggtgctattt atcgctttga accattagta taaatatacg taagtgctat
      841 gagcgagaat gcccatatga ataatgacaa gcacaatgga aagaatcgtt aatatattat
      901 ttaatcgtga tgacttaatt aaaatgaaaa agattgataa tataaatgtg aaaaagataa
      961 gtataacccg taaactaaag taattcacgg tgagaggttg actcaatgtc ataatgattg
     1021 caacgatgtt cataattata aatagactta aaataattgt tctcatatca aacacctcat
     1081 tgttagatta ttgacattat aacaggggta attgtatatg aacattaatg tggttgcttg
     1141 aggaaaaatt tattcattga agtcaagttg gttcatttta gaaatgaata tcgtgttaga
     1201 tgatgaaagt atattgaagt ataggtaact agttgaaaag tattaattgt acgataacat
     1261 taaatttaac acgaaacata gatataaaat gattcacaat taaaatgggt aaatttgaac
     1321 ttgctaaact attaattgga gcatggacat ttcaaaaata agagttcaaa tcttacacaa
     1381 gctctgaatc gacactataa gatacaaact gtataattaa aggtattgtt aaatagaagg
     1441 agatatcata aatcatggaa aagatgcata tcactaatca ggaacatgac gcatttgtta
     1501 aatcccaccc aaatggagat ttattacaat taacgaaatg ggcagaaaca aagaaattaa
     1561 ctggatggta cgcgcgaaga atcgctgtag gtcgtgacgg tgaagttcag ggtgttgcgc
     1621 agttactttt taaaaaagta cctaaattac cttatacgct atgttatatt tcgcgtggtt
     1681 ttgttgttga ttatagtaat aaagaagcgt taaatgcatt gttagacagt gcaaaagaaa
     1741 ttgctaaagc tgagaaagcg tatgcaatta aaatcgatcc tgatgttgaa gttgataaag
     1801 gtacagatgc tttgcaaaat ttgaaagcgc ttggttttaa acataaagga tttaaagaag
     1861 gtttatcaaa agactacatc caaccacgta tgactatgat tacaccaatt gataaaaatg
     1921 atgatgagtt attaaatagt tttgaacgcc gaaatcgttc aaaagtgcgc ttggctttaa
     1981 agcgaggtac gacagtagaa cgatctgata gagaaggttt aaaaacattt gctgagttaa
     2041 tgaaaatcac tggggaacgc gatggcttct taacgcgtga tattagttac tttgaaaata
     2101 tttatgatgc gttgcatgaa gatggagatg ctgaactatt tttagtaaag ttggatccaa
     2161 aagaaaatat agcgaaagta aatcaagaat tgaatgaact tcatgccgaa attgctaaat
     2221 ggcagcagaa gatgaaaaca tctgaaaagc aagctaaaaa agcgcaaaat atgattaatg
     2281 atgcgcaaaa taaaattgct aaaaatgaag atttaaaacg agacctagaa gctttagaaa
     2341 aggaacatcc tgaaggtatt tatctttctg gtgcactatt aatgtttgct ggctcaaaat
     2401 catattactt atatggtgcg tcttctaatg aatttagaga ttttttacca aatcatcata
     2461 tgcagtatac gatgatgaag tatgcacgtg aacatggtgc aacaacttac gatttcggtg
     2521 gtacagataa tgatccagat aaagactcag aacattatgg attatgggca tttaaaaaag
     2581 tgtggggaac atacttaagt gaaaagattg gtgaatttga ttatgtattg aatcagccat
     2641 tgtaccaatt aattgagcaa gttaaaccgc gtttaacaaa agctaaaatt aaaatatctc
     2701 gtaaattaaa acgaaaatag attaacgact gaaatctgaa cgctcataag actgtcattt
     2761 gcgttcagat ttttttacac aatatagaat ggttgagtaa aatatttttg aatatagtga
     2821 aagaggggga agtactgtga taaaaaagct attacaattt tctttaggga ataagtttgc
     2881 tatcttttta atggttgttt tagttgtctt gggcggtgta tatgcgagtg ctaaattgaa
     2941 attagaatta ctaccaaatg tacaaaatcc agttatttca gttacaacaa caatgccggg
     3001 tgcaacgcca caaagtaccc aagatgaaat aagtagtaaa attgacaatc aagtaagatc
     3061 attggcatat gtgaaaaatg ttaaaacgca atccatacaa aatgcttcaa ttgtaacagt
     3121 tgaatatgaa aataatacag atatggataa agcagaagaa cagcttaaaa aagaaatcga
     3181 taaaattaaa tttaaagatg aagttggtca accagaatta agacgtaatt cgatggatgc
     3241 ttttccggtt ttagcatatt cattttcaaa taaagagaat gacttgaaaa aagtaacgaa
     3301 agtactgaat gaacaattaa taccaaaatt gcaaacggta gatggtgtgc aaaatgcgca
     3361 attaaatggg cagacgaacc gtgaaatcac ccttaaattt aagcaaaatg aacttgaaaa
     3421 atatgggttg actgctgatg atgtagaaaa ctatctaaaa acggcaacaa gaacaacgcc
     3481 acttggattg ttccaatttg gtgataaaga taaatcaatt gttgttgatg gtcaatatca
     3541 atctgttgat gcttttaaaa acataaatat tccattaacg ttggcaggag gacaagggca
     3601 atctcaatcc caaagtgaca ataaacaaaa ttcagccatg tcagacgtta atcaggcatc
     3661 accacagcaa aattcaaaag cgtcagcacc aaataatata agtggtatgc cgacagcgaa
     3721 actaggagat ttagctgata ttacagttgg tgatgtgcgt acttctattt ctaaaacgaa
     3781 tggaaaggat gcggttaatc tacaaataac taaagctcaa gatgccaata cagttcaagt
     3841 agccaaagat gtacaacgta aaattgatac attcgttgat gaaaataaag attttaatgt
     3901 cacaaaaaca atggatactg caaagcctgt tgagaaatca ctttatacga tggttgaaaa
     3961 agcatcatta ggtacaatcg tggcaattat agttattttg ctgtttttaa gaaacattcg
     4021 tacgacggca atttctatta tatcgattcc gttatcactt cttatggcgc ttattgctct
     4081 gaaattgagt gatgtttcat tgaatatact aacgttaggt gcattaacag tagcgattgg
     4141 acgtgtgata gacgattcga ttgtagttgt tgaaaatatt tatcgacgct taacagattc
     4201 agaagaacaa ctaaaaggtg aaaatttaat tatcagtgcg acaactgaag tatttaaacc
     4261 aataatgtca tcgacactag ttactattat cgtcttctta ccacttgtgt ttgtatcagg
     4321 ttcagtaggc gaaatgttta gaccttttgc attggctatt gcatttagtt tattagcatc
     4381 gttattagtg tcaattacac tcgttccagc gttggcagct acactattta aaaaaggcgt
     4441 taaacgtcgt aataaacaac atcaagaagg attaggtgtt gttagtacaa cttataaaaa
     4501 agtattacat tggtcattaa atcataagtg gattgtaatt atattaagta cattaatttt
     4561 ggttgcaact attgtatttg gaggaccgag actaggcact agctttattt cagcaggtga
     4621 cgataaattt ttagctatta cttatacacc gaagcctggt gaaacggagc aagcagtgtt
     4681 gaatcatgcg aaagatgttg aaaaatattt aaaacagaaa aagcatgtaa aaacaattca
     4741 atactcagtt ggcggtagta gtccagtaga tccaacgggt agtacaaata gtatggcaat
     4801 catggttgaa tatgataatg acacgcctaa ttttgatgta gaagcggata aggttattaa
     4861 acatgcagat ggctttaaac atcctggaga gtggaaaaat caagatttag gaacaggtgc
     4921 aggtaataaa tctgtagagg ttactgtaaa aggtccatca atggatgcca taaaatcaac
     4981 tgtaaaagat attgaacaga aaatgaaaca ggttaaagga ctagccaatg tcaaatctga
     5041 tttatcgcaa acatatgatc agtatgaaat taaagtcgat caaaataaag cggcagaaaa
     5101 tggtatttct gcaagtcaac ttgcaatgca cttgaatgaa aacttaccag aaaaaacagt
     5161 tacgactgtt aaagaaaatg gtaaaactgt tgatgttaaa gtcaaacaaa ataagcaaac
     5221 agactggtca gaagataagt tgaataatat aactttgaaa aagccgactg gtggtacgat
     5281 taaattggga gatatcgcta cgttagttaa aacaacgaca ccaagtaaat tgacgcaaga
     5341 acaaggagat tatgcaacga cggtatctgc taaagtaaca aataaagatg tgggtggcac
     5401 aacacgacaa gtgatgtcta aaataaataa tttggacaaa ccgaataatg taaaggttaa
     5461 tatcggtggt gcatcagatg atattaacaa tgcaatgaca caattagcct ttgcaatgtt
     5521 agctgcaatc attatcgtat atttaatcct agttattaca tttaaaggtg gcctagcacc
     5581 atttacaatt ttattctctt taccatttac agttatcggt gtaattattg cactattaat
     5641 cacaggagaa acaatatcag taccaagttt aattggtatg ctaatgttaa ttggaatcgt
     5701 agtaacaaat gccattgtgt taatagaccg tgttattaat aatgagcaac agggcatgga
     5761 gatgaaagaa gcattaatcg aagcaggcgg tactagaatt agaccgatat taatgacggc
     5821 gattgcaaca attggtgcat tagttccttt gttatttggt caagatagct cgattcttat
     5881 ttcgaaagga ttagctgcta cagttattgg tggtttaatt tcatcaactt tattaacact
     5941 tgtagttgta ccagtaatat atgaaattct ctttacttta aaaaaacgat tcactaaacg
     6001 atagataata taaaagccat aaaagcggtc atgatattga ctacttttat ggctttttaa
     6061 aatttgaaat tttagacctt atttcacgtg cgatttaatc gatgtgttaa taaaacaata
     6121 ctggcattca acatgtaatc cgataataaa ataaggttgc aattatgtta ttgcatattt
     6181 tgtttattac ttagaataat acgatggtta agatgaaata tatgacaaag ataaataatg
     6241 acattaaatt caagatgttt acgcctttta gatctttttt aagcatttta aatcctgtca
     6301 cttcgacatt accgactaag agtaataaga cgataaatac ccaccaattc tcttttggaa
     6361 aactcatcgt cgttaatgct aagacgaata ataacaacac acccataata attctcaaaa
     6421 tgcgagtaaa tacaacttcc attttaaaaa tactaataaa actaacaatt agataaagta
     6481 ttgctagtaa aatcagataa aattgcatat gataacttcc tctccgtttc aaagggttca
     6541 tgtagtacag cagcgtagaa aacataaata gcagtgttgg gatagatatg cgtgtatgtt
     6601 gttaatgaaa cactaaatca taacacgaca acatcttgtg attctagatg taattgagcg
     6661 aaaaatgaag caattcatca atataacgta ttcagagcaa tgctagtcaa atttatgtaa
     6721 caaaaagcat ttattgcttg aatgatagat tattaatctt gcatttgagc acgaacgttt
     6781 ctaatttcat tttcaatatc tttaagttga cgaacaagtg cggaagtatc taaattcaaa
     6841 gattcccgtt tttctatgtc accttgaaga cgaacataat catatttaag ttcggctagt
     6901 tggttttcta aagacatact ataacactcc tttgaaatat aattaagtgc atttaatgta
     6961 gattatagca tgaaacagta gacttctaaa atatctgtgg agtatgttaa agttataaat
     7021 tatgaagatt attgaaagaa gggataacag tgacagaaca atttactatg attattttat
     7081 taattgcgct tggttacttt ttgaagcgca ttaattttat aaaagcaaca gatagtcaag
     7141 tcattgctac attagttctt aatgtcacac tcccatcttt agttattgtg aatttaaaca
     7201 gtgcagaatt aaaattgtca ttctccattt taccaatttt aatgattata tatggtattg
     7261 tggcgaaaat gattgctgtc tggtttttta gaaaatatga taatcacatg cgtggatcag
     7321 taggtatgat gactggtgct atgaatattg gattgtttgc atatccacta attgaagtga
     7381 tatggcctaa aacgggactt atttattttg ttatggctga tataggtggt gcctttgtga
     7441 tgtttggaat tacttacttt gtaggtagct actttagtga aggtagtgat caattcgatt
     7501 ttaaatattt aggtaagaaa ttattacaat cagtgccact cgttacatac attgtaatgt
     7561 ttatattgaa tataagtcac atccatttac cacatgtggc aatcgatttc tttagcatac
     7621 tttcccatgc aaatatgccg ttatcaatga ttttgctagg tgtcatgttg aattttactc
     7681 tggaaagaaa gtatatacct gctactatta aatatttatg tttgcattat ggattagcat
     7741 tagttgcggg attgttagta tatgtgttgt taccagtatc agatgatatg attaagacga
     7801 cattgttggt cacttggatg ttcccggtag gtgttgcaat tatttcttat ggtattcagt
     7861 ttaaatatcg aacactgaca tttataggta tgacgaccaa tttgacaatc attattagta
     7921 tcattatttt gtacgtgtat caggcagtgt ttgtctagtt tacacatttg aaaataaaat
     7981 ccattttagt attcgtagaa aatgagagcc ccctttacca caatatgtaa atgtatattg
     8041 tgaaaagggg gcttttattt atgacatagt tgttcaactc atacattcaa gttgataatg
     8101 cagtgcgtta tgcagctatc cacttcatat gtatggcgca tacttgatat atgaatatca
     8161 tcaagatttt aaaagtatcg ctaattttaa agtcttaaaa cgcatgatta atgtcatgat
     8221 gtgttattgc gttttgtttt tagagccacg tatttcctag gacatataga ggaacattca
     8281 acgtaaaatt cccgaaaatt aattttaatc attctataag tgaatggtta ttttcagaag
     8341 tgattacatt tttacgttac atgagtattt aactttctat ttcaaattac ctagaattat
     8401 agcagctatc acgataataa taatacctgc ccaaataccc gtcatctgac gacgatcttt
     8461 tctttctcct aaaatgaaaa taccgcctaa ggttgaaacg ataacaagta actgtgataa
     8521 tgagaaactt gtagctacac caacttttgg ttgagaatag aacatgaaca agttaccaat
     8581 tccccaaatc acacctggca atagattaag tgctgttgat ttaagtgatg ttttatgatt
     8641 catggatagg ataaagccac caattgccat accgacagat tggaagaaca atgcatcagt
     8701 tccaccaaca ccaaagatgt caccaagtac aacgaaacct acatatccaa ctgtagatac
     8761 aattaaaata cccattgctt ttttaaattc aggattatct gattgacgtt catttttagc
     8821 tttaagtgaa gtaagtgcta caccagtaac taataatatc atggcgatta aaccaaagat
     8881 aatttgagtc attgaagacc attcacctaa aaagataacg ctgaataatg ttgtaccaac
     8941 taattgcata cctgtagaaa ctggcatagt atttgataca ccgattaaac taatagattt
     9001 aagctgattc gcttgtccaa acgcccataa tgcaccagaa ataagaccga caataattac
     9061 agtaggatta ttgaattttg cgaaaccagt aataagtaaa cctaatccaa caatgagtgc
     9121 gcctagcgtc gtaccacgaa tttggttgta aggtccaccg ccgacgaaca cattaataag
     9181 aacgacactt ccccagaata aagcaggtaa aagtgcgatt aagaaatcaa gaaattgcaa
     9241 gactactcac tctcctatac tatttatgca agtgctcccc ttaattgaca cttgcatgtt
     9301 attgtaaagc attctgccct aatagtaaaa aacaactact tataattttc aatatttaca
     9361 aaaagtagaa aaacccacaa tacatgctaa tgatgtactg tagggtattt gcttggtgtt
     9421 ttattcaggg attgtttgat cttttacgtt acgtgattcg aatttgaatt taatgtactc
     9481 atagttgata tggaaaccta gaccattaaa tcgttcgatg agtttattat ttttattacg
     9541 aattttaaaa taaacttttc taatatcaaa attatcaaaa gcatattgca atgcatacgc
     9601 aagcaactcg aaagcgatac ctttcaaacg gtaatctaca tgagaactga agtatttaat
     9661 ctcggcgatt gactgttgtg aatcaatttc aaggtataaa taacctttaa gtaaaccttc
     9721 gctaacaaat aaaaacaaat gatgatggtc gtctaaacta tcaatgatat cttgtggtga
     9781 ctgtgcctga tatttaaatg tagttgtgtg taatttgctg aaagcacgat aaaacccttt
     9841 gtgataagga atgatatttg gttggtgcat atcttcttct aatcttgtac gggcttctat
     9901 gtaatagtca gtgaagttat aacttgcttg aataactttc attaatggtt tgtattgttg
     9961 aatgccttct tcaaaagaaa agttaaagac ggcatcgtca ggttggttcg aagtcattgc
    10021 tttaaatagc gtttcaaacg tatcgacaga taatacataa ttgtcagcca cgaaagggcc
    10081 tatgactttg aatttatttt tatcgtatgc aaaagacaat atgattgctt tgattgaatc
    10141 gttttcttta atcgaaaata caccaggtga ttcaatcgca tcggttatta atacatctat
    10201 ttcattgtac tgttgaggaa gtttatataa gtatgatgct gattcataat tcgaatgttc
    10261 aatgaacgat tttatttgtg taatatcata taactgaact atgctcatgt cattacctcc
    10321 gtactttttg ttacttttat tatatagtat ttcaactgaa atgaaagtta atagtgatat
    10381 taacatgtta caatacattt aacacatcat ttgcaatcat tgaatttaaa tcaaagatta
    10441 gtggaataga tgaagcacgt tcgaaataaa agaacgtatg agaaaggata atttatgaaa
    10501 tctttaatat tagctgaaaa accatcagtc gcaagagata ttgctgatgc tttacaaata
    10561 aatcagaagc gtaatggtta ctttgaaaat aaccaatata ttgtcacgtg ggcgttaggt
    10621 catctagtga caaatgcgac acctgaacaa tacgataaaa atttaaagga atggcgatta
    10681 gaagaccttc caattatacc taaatatatg aaaactgttg ttattggtaa aacaagcaaa
    10741 caatttaaaa cagtaaaagc gttaatttta gataataaag tgaaagatat tattattgca
    10801 acagatgctg gacgagaagg tgaactagtt gcaagattga ttttggataa agttggtaac
    10861 aaaaagccaa tccgtcgatt atggattagc tcagttacta aaaaagctat tcaacaaggt
    10921 tttaaaaatt taaaagacgg tcgtcaatat aacgatttgt attatgcagc gttagcgaga
    10981 agtgaggcag attggattgt tgggattaat gcaacgcgtg cactaacaac aaagtatgat
    11041 gcacagctat ccctgggacg tgttcagaca ccaacgattc aattagtaaa tacacgacaa
    11101 caagagatta atcagttcaa accacaacaa tactttacat tatcattaac ggtaaaaggg
    11161 tttgattttc agctagaatc aaatcagcga tataccaata aagaaacttt agaacagatg
    11221 gttaataatt tgaaaaatgt cgatggtaag attaaatctg ttgctactaa acataagaag
    11281 tcgtatccgc aatcactgta caatttaaca gatttacaac aagatatgta tagacgttat
    11341 aaaattggac ctaaagaaac attgaataca cttcaaagct tatatgagag acataaagtc
    11401 gtaacctatc caagaacaga ttcaaactat ttaacaactg atatggtaga tactatgaaa
    11461 gaacgtattc aggcgacgat ggcaacaaca tataaagacc aagcacgccc attaatgtct
    11521 aaaacatttt catcaaaaat gtcgatattt aataatcaaa aagtatctga tcaccatgca
    11581 attattccta cagaagtgag acctgtcatg tcagacttaa gtaatagaga attaaagtta
    11641 tacgatatga ttgtcgagcg ttttttagaa gctttaatgc ctccgcacga gtatgacgcg
    11701 ataactgtaa ctttagaggt tgcagggcac acatttgttt tgaaagagaa tgtaacaact
    11761 gttttaggtt ttaaatctat tagacaaggt gaatctatta cagagatgca acagcctttt
    11821 tcagaaggcg atgaagtgaa gatttcaaaa acaaatatta gagaacatga aacaacacct
    11881 ccagaatatt ttaatgaagg ttcgttatta aaagcgatgg agaaccctca gaactttatt
    11941 caattgaagg ataaaaaata tgcgcaaact ttaaaacaaa caggtggtat cggcacagtt
    12001 gcaacaaggg ccgacattat cgataaatta tttaatatga atgccattga atcaagagac
    12061 ggtaaaatta aagtaacgtc aaaaggtaaa caaatattag aattagcacc agaagaatta
    12121 acgtcgccac ttttaactgc acaatgggaa gaaaaattac ttttaattga acgtggtaaa
    12181 tatcaggcga aaacatttat taatgaaatg aaagatttta cgaaagatgt tgtaaatggg
    12241 attaaaaata gtgatcgtaa atataaacac gataatttaa caaccacaga atgcccaacg
    12301 tgtggtaaat tcatgattaa agttaaaact aaaaatggtc agatgcttgt gtgccaagat
    12361 ccatcttgta agacgaaaaa gaatgtacag cgcaaaacaa atgcaagatg tccaaactgt
    12421 aaaaagaaat taacgttgtt tggtaaaggg aaagaagcgg tatatcgttg tgtttgtgga
    12481 cattctgaaa cgcaagcaca tatggatcag cgtatgaagt ctaaatcctc tggtaaagta
    12541 tctcgtaaag aaatgaaaaa gtatatgaat aaaaatgaag gtttagacaa taatccgttt
    12601 aaagatgcat taaagaactt gaatttatag ataaaatcga acaaagttga atcagaaaaa
    12661 cgaaaagttc gcttttggta ttgtttttta ttaagaatga tattaaacta ttaaggtatt
    12721 ttaaaaaaag gagcatccat tcgtgaaaaa ctatttccag ttcgataaat atggaacaaa
    12781 ctttaaaaga gaaatcttag gcggtatcac aactttctta tctatggcct atattttagc
    12841 agttaacccg caagttttaa gtttagcagg tgttaaaggc gtatcagaag atatgaaaat
    12901 ggaccaaggt gccatttttg tagcgactgc attagcagca tttgtaggct cgctattcat
    12961 ggggctaata gctaaatatc caattgcgtt agcaccaggt atgggattaa atgcattctt
    13021 tgcatttact gttgtgttaa ccatgggtat tccttggcaa gttggtttaa caggcgtttt
    13081 attctcaggt atattctttg cgatattaac cgtcacaggg tttagggaag ttattattaa
    13141 tgcaattcct tatcaaatga agatggctgt ttcagcaggt ataggattat tcattacatt
    13201 tgtaggttta caaagttcgg gtattatagt taaaaatgaa tctacattag taacattggg
    13261 acatttaact gatccaccag tacttttggc gatattcggt atcgttatta cagttatttt
    13321 atatgcaatt aaattgccgg gttctatctt tatcggtatg atcatcacag caatcgttgg
    13381 tatgttcact ggattgattc aaatgccatc tggtattgtg ggtaagattc caagtattga
    13441 accaacgttc ggtgctgcat ttgaagcgtt taaagaccca agtcaattat taacgataca
    13501 gttcttaatt gttattttaa cgttcttatt tattgatttc tttgatacag ctggtacatt
    13561 agtagctgtt gctactcaag caggtattat gaaagataat aaattaccaa gagctggtag
    13621 agcattgttc tcagattctt tagctacgat tgtaggatca gtttttggaa caactacaac
    13681 aacttcttat atcgaatcaa cttcaggtgt tgctgtaggt gctagaacag gatttgctag
    13741 tatcgtaaca ggttgttgtt tcctattagc gctgttcttt agtccgttaa tcgctgttgt
    13801 aacaagtgca gttacgacac cagccttagt ggtggtaggt gtattaatgg ctgcaaactt
    13861 tgctgagata aactggaaaa gttttgaagt tgcagtgcca gccttcatta caatcattat
    13921 gatgccttta tcatattcaa ttgcaactgg tattgcgtgt ggatttattt tctatccaat
    13981 tacaatgctt atctctaaaa aacataaaga agtacatccg ataatgtatg tattaatggt
    14041 actattcatt ctgtacttca tatttgttca tggttaaatc cagacacaaa aaacgactgc
    14101 caatgattgg tagtcgtttt tttctttgaa gtaaggataa aataagaagc gaaataacat
    14161 aaagcgatat tatgaatgtc tttaaatatc cgatataaat atacttcttt taaaattgtt
    14221 ggacgtgttt cttctatatt atatacctaa ttaaaaaggt tttaagaatt attctctatg
    14281 tccatctttg tattgtgtgt aaatgacacg agcattttta ttaggtatat aggttggcgc
    14341 tgataataca actaggacaa atagaatcat taggaaaaat aaaatccaat gaatcaaaat
    14401 tcctaagaag gggataatag ctaaaaaact acctactata ccaaatattg gaataatcgc
    14461 tataggtcta atcgcatttt gtctattcac taataatata atagtaagaa ttagataaag
    14521 taatgcatta aagagtaaag gctgccatcc aaaacttaaa ataaacgttg cacctaaaaa
    14581 aggaatgcca tagaaaaact cacaaagtat taaaaagata cctataagtg ctaaagataa
    14641 ttttggtcca tttttcatat tcattcacca ccgttcttat gactaattat atagaataat
    14701 tttcaatgat aataataaaa tgattcaagg tacgaattta caataaagaa ggaaataaaa
    14761 aatatctcga aaatagttga actgactaag tatataaagt aaaatataaa agtatgtgtt
    14821 agacagatag aaaaattatg tgaaaagcct tgatttatcg atgtttttcg tgtaatataa
    14881 ctaacgttgg actttaagaa aagcgatgaa gcgaaaggtt actgacacac ccggccgctt
    14941 tgccatggcg ctgtgtaaga tagttttcgt ggagaagtct atcactaaat gtagacgaat
    15001 aaggagggaa aattatggca aaacaaaaaa tcagaatcag attaaaagct tatgatcacc
    15061 gcgtaattga tcaatcagca gagaagattg tagaaacagc gaaacgttct ggtgcagatg
    15121 tttctggacc aattccgtta ccaactgaga aatcagttta cacaatcatc cgtgccgtgc
    15181 ataagtataa agattcacgt gaacaattcg aacaacgtac acacaaacgt ttaatcgata
    15241 ttgtaaaccc aacaccaaaa acagttgacg ctttaatggg cttaaactta ccatctggtg
    15301 tagacatcga aatcaaatta taatagacaa ttttaggagg tggactttcg atgaccaaag
    15361 gaatcttagg aagaaaaatt gggatgacac aagtattcgg agaaaacggt gaattaatcc
    15421 ctgtaacagt agtagaagct aaagaaaatg ttgtattaca aaagaaaact gtagaagttg
    15481 atggatacaa cgcaatccaa gttggatttg aagacaaaaa agcatacaaa aaagatgcaa
    15541 aatctaataa atatgctaat aaaccagctg aaggtcacgc taaaaaagct gacgcagcac
    15601 ctaagcgctt cattcgtgaa ttccgcaatg tagacgtgga tgcttacgaa gtaggtcaag
    15661 aagtctcagt agatactttt gtagctggcg acgttattga cgtaacaggc gtatcaaaag
    15721 gtaaaggttt ccaaggtgca attaaacgcc acggacaatc tcgtggacct atgtcacacg
    15781 gttctcattt ccacagagca ccaggttctg taggtatggc ttcagatgct tctagagtat
    15841 ttaaaggcca aaaaatgcca ggacgtatgg gtggaaacac tgtaactgtt caaaacttag
    15901 aagtagttca agttgacaca gaaaacaaag ttatcttagt aaaaggtaac gtacctggac
    15961 ctaaaaaagg tttagtagaa atcagaactt caattaaaaa aggtaataaa taataagaag
    16021 tgaaaggagg aaatgcataa tggctaatta tgatgtttta aaattagacg gaactaaatc
    16081 aggttcaatc gaattaagcg atgcagtatt cggtattgag ccaaataata gcgttttatt
    16141 cgaagctatt aatttacaac gtgcttcatt acgtcaaggt acgcatgctg ttaagaatcg
    16201 ttcagcagta agcggtggcg gacgtaaacc atggaagcaa aaaggaacag gtcgtgctcg
    16261 tcaaggtaca atccgtgctc cacaatggcg tggcggtggt atcgtattcg gaccaactcc
    16321 aagaagttat gcatacaaaa tgcctaagaa aatgcgtcgt ttagctttac gctcagcatt
    16381 atctttcaaa gctcaagaga atggcttaac tgtagttgac gcattcaact tcgaagctcc
    16441 aaaaactaaa gaattcaaaa atgtattatc tacattagaa caacctaaaa aagtattagt
    16501 agttactgaa aacgaagatg taaatgttga attatcagca cgcaacatcc ctggcgttca
    16561 agtgacaact gctcaaggtt taaatgtttt agatatcact aatgctgaca gcttagtaat
    16621 tactgaagct gctgctaaaa aagttgagga ggtgctcgga taatggaagc aagagatatt
    16681 cttaagcgcc ccgtaatcac tgagaaatct tctgaagcaa tggctgaaga caaatacact
    16741 ttcgacgttg atactcgtgt taacaaaaca caagtaaaaa tggcagttga agaaatcttc
    16801 aacgtaaaag ttgcaagtgt taatatcatg aattacaaac ctaagaaaaa acgtatgggc
    16861 cgttaccaag gctatacaaa caaaagaaga aaagcgattg taactcttaa agaaggatca
    16921 atcgacttat ttaactaata aaacaattac cattaaggag gtaagcgaca atggctatta
    16981 aaaagtataa gccaataaca aatggtcgtc gtaatatgac ttcgttagat ttcgcagaaa
    17041 tcacgaaaac tacacctgaa aagtcattat taaaaccgct accgaaaaaa gcgggacgta
    17101 acaaccaagg taaattgact gtaagacacc atggtggtgg acacaaacgt caataccgtg
    17161 ttatcgattt caaacgtaac aaagatggta tcaatgcaaa agttgattct attcaatatg
    17221 atccaaaccg ctcagcaaac atcgctttag ttgtatatgc agacggtgaa aaacgatata
    17281 tcattgctcc taaaggatta gaagtaggtc aaatcgttga aagtggtgct gaagctgaca
    17341 tcaaagttgg taacgcatta ccattacaaa acattccagt tggtacagta gtacacaaca
    17401 tcgagcttaa acctggtaaa ggtggacaaa tcgctcgttc agctggtgca agtgctcaag
    17461 tacttggtaa agaaggtaaa tacgtattaa tcagattaag atctggtgaa gttcgtatga
    17521 tcttatctac ttgccgtgct acaatcggtc aagttggtaa cctacaacac gaattagtta
    17581 acgttggtaa agccggacgt tcaagatgga aaggtatccg tccaacagtt cgtggttctg
    17641 taatgaaccc taacgatcac ccacacggtg gtggtgaagg tcgtgctcct atcggtagac
    17701 catctccaat gtcaccatgg ggtaaaccta cgcttggtaa gaaaactcgt cgtggtaaaa
    17761 aatcatcaga caaacttatc gttcgtggac gtaagaaaaa ataatataat caacttattt
    17821 gggtgtgcgg cttaaagctg cacgcacata ataagaaggg aggcgcccaa atggctcgta
    17881 gtattaaaaa aggacctttc gtcgatgagc atttaatgaa aaaagttgaa gctcaagaag
    17941 gaagcgaaaa gaaacaagta atcaaaacat ggtcacgtcg ttctacaatt ttccctaatt
    18001 tcatcggaca tacttttgca gtatacgacg gacgtaaaca cgtacctgta tatgtaactg
    18061 aagatatggt aggtcataaa ttaggtgagt ttgctcctac tcgtacattc aaaggacacg
    18121 ttgcagacga caagaaaaca agaagataat atctattaag tagaggagga catcctaatg
    18181 gaagcaaaag cggttgctag aacaataaga atcgcacctc gtaaagtaag actagttctt
    18241 gacttaatca gaggtaaaaa tgctgctgaa gctattgcaa ttttaaaatt aacaaacaaa
    18301 gcttcatcac cagtaattga aaaagtatta atgtccgctt tagctaatgc tgaacataac
    18361 tatgacatga acacagatga attagtagtt aaagaagcat atgctaacga aggaccaaca
    18421 ttaaaacgtt tccgtccacg tgcgcaaggt cgtgcaagtg cgattaacaa acgtacaagc
    18481 cacattacaa tcgtcgtaag tgacggtaaa gaagaagcta aagaagctta attaactttt
    18541 aaggagggaa tactgtgggt caaaaaatta atccaatcgg acttcgtgtt ggtattatcc
    18601 gtgattggga agctaaatgg tatgctgaaa aagacttcgc ttcactttta cacgaagatt
    18661 taaaaatccg taaatttatt gataatgaat taaaagaagc atcagtttct cacgtagaga
    18721 ttgaacgtgc tgcaaaccgt atcaacattg caattcatac tggtaaacct ggtatggtaa
    18781 ttggtaaagg cggttcagaa atcgaaaaat tacgcaacaa attaaatgcg ttaactgata
    18841 aaaaagtaca catcaacgta attgaaatca aaaaagttga tcttgacgct cgtttagtag
    18901 ctgaaaacat cgcacgtcaa ttagaaaacc gtgcttcatt ccgtcgtgta caaaaacaag
    18961 caatcactag agctatgaaa cttggtgcta aaggtatcaa aactcaagta tctggtcgtt
    19021 taggcggagc tgacatcgct cgtgctgaac aatattcaga aggaactgtt ccacttcata
    19081 cgttacgtgc tgacatcgat tatgcacacg ctgaagctga cactacttac ggtaaattag
    19141 gcgttaaagt atggatttat cgtggagaag ttcttcctac taagaacact agtggaggag
    19201 gaaaataata atgttactac caaaacgtgt aaaatatcgt cgtcaacatc gtcctaaaac
    19261 aactggtcgt tctaaaggcg gtaactacgt aacatttggt gagtttggtt tacaagctac
    19321 aacaacgtct tggatcacat ctcgtcaaat cgaatctgct cgtatagcaa tgacacgtta
    19381 catgaaacgt ggcgggaaag tttggattaa aatcttccca catacaccat atactaaaaa
    19441 acctttagaa gtacgtatgg gtgctggtaa aggtgcggtt gaaggctgga tcgcagttgt
    19501 taaaccaggt agaattttat tcgaagttgc tggcgtttct gaagaagttg cgcgtgaagc
    19561 actacgttta gcaagtcaca aacttccagt aaaaactaag tttgtaaaac gtgaggaatt
    19621 gggtggtgaa acaaatgaaa gctaaggaaa ttagagactt aaccacttca gaaatcgaag
    19681 aacaaatcaa atcttcaaaa gaagagcttt ttaacctacg ctttcagtta gctacaggtc
    19741 aattagaaga aactgcacgt attcgtacag taagaaaaac gattgcacgt ctaaaaactg
    19801 ttgctcgtga aagagaaatt gaacaaagta aggctaatca ataattaagt aagaggaggt
    19861 tacaaaagtg agcgaaagaa acgatcgtaa agtttatgta ggtaaagttg tttcagacaa
    19921 aatggacaag actattacag tacttgttga aacttacaaa acacacaaat tatacggtaa
    19981 acgagtaaaa tactctaaaa aatacaaaac tcatgatgaa aacaattcag ctaaattagg
    20041 agacattgtt aaaattcaag aaactcgtcc tttatcagca acaaaacgtt ttcgtttagt
    20101 agagattgtt gaagagtcag taattattta atacaagttt agagataagg gaggtttaac
    20161 taatgatcca acaagaaaca cgcttgaaag tagcagacaa ctctggtgct cgtgaagttc
    20221 ttacaatcaa agtattaggt ggatctggtc gtaaaacagc aaacatcggc gatgttatcg
    20281 tatgtactgt taaaaatgca acaccaggtg gcgttgttaa aaaaggtgac gttgtcaaag
    20341 ctgtaatcgt acgtactaag tcaggtgttc gtcgtaatga cggttcatac atcaaatttg
    20401 atgaaaatgc atgtgttatc atccgtgatg acaaaggccc acgtggtact cgtatcttcg
    20461 gacctgttgc tcgtgaatta cgtgaaggta acttcatgaa aatcgtatca ttagcaccag
    20521 aagtacttta attaataaca accaatacaa ataaggaggt gcccacatgc atatcaaaaa
    20581 aggtgacaac gttaaagtta tcgcaggtaa agacaaaggt aaagaaggta aagtaattgc
    20641 tactctacct aaaaaagacc gtgtcgttgt ggaaggtgtt aacattatga aaaaacacca
    20701 aaaaccaact caattaaatc ctgaaggtgg aatcttagaa acagaggcag caatccatgt
    20761 ttctaatgta caattattgg accctaaaac aaacgaacca actcgtgtag gttacaaatt
    20821 tgttgatggt aaaaaagttc gtatcgctaa aaaatctggc gaagaaatta aatctaataa
    20881 ttaataacaa gttgaaagga ggatcccact ttgaaccgtt taaaagaaaa gtttaacact
    20941 gaagttactg aaaacttaat gaaaaaattc aattatagtt cagtaatgga agtaccaaaa
    21001 atagataaaa tcgttgtgaa catgggtgta ggtgacgcag tacaaaattc taaagtatta
    21061 gacaatgctg ttgaagaatt agaattgatc actggtcaaa aaccattagt aactaaagct
    21121 aaaaaatcaa tcgcgacttt ccgtttacgt gaaggtatgc caatcggtgc gaaagtaaca
    21181 cttcgcggtg aaagaatgta tgaattctta gacaaattaa tttcagtatc attaccacgt
    21241 gtacgtgact tccaaggtgt ttctaaaaaa gcatttgacg gacgcggtaa ctacacttta
    21301 ggtgttaaag aacaattaat tttcccagaa atcgactatg ataaagtaag taaagttaga
    21361 ggaatggata ttgttatcgt aacgactgct aacactgatg aagaagctcg tgaattgtta
    21421 gctaacttcg gtatgccatt ccgtaaataa ttatttaaag gaggctaatt aagtggctaa
    21481 aacttcaatg gttgctaagc aacaaaaaaa acaaaaatat gcagttcgtg aatacactcg
    21541 ttgtgaacgt tgtggccgtc cacattctgt atatcgtaaa tttaaattat gccgtatttg
    21601 tttccgtgaa ttagcttaca aaggccaaat ccctggcgtt cgtaaagcta gctggtaata
    21661 aaaaagagtc tgaaaggagg caacaatcaa tgacaatgac agatccaatc gcagatatgc
    21721 ttactcgtgt aagaaacgca aacatggtgc gtcacgagaa gttagaatta cctgcatcaa
    21781 atattaaaaa agaaattgct gaaatcttaa agagtgaagg tttcattaaa aatgttgaat
    21841 acgtagaaga tgataaacaa ggtgtacttc gtttattctt aaaatatggt caaaacgatg
    21901 agcgtgttat cacaggatta aaacgtattt caaaaccagg tttacgtgtt tatgcaaaag
    21961 ctagcgaaat gcctaaagta ttaaatggtt taggtattgc attagtatca acttctgaag
    22021 gtgtaatcac tgacaaagaa gcaagaaaac gtaatgttgg tggagaaatt atcgcatacg
    22081 tttggtaata aaaaataagg aggtgccata acatgagtcg tgttggtaag aaaattattg
    22141 acatccctag tgacgtaaca gtaacttttg atggaaatca tgtaactgtt aaaggtccta
    22201 aaggtgaatt atcaagaact ttaaatgaaa gaatgacatt caaacaagaa gaaaacacaa
    22261 ttgaagttgt aagaccatct gattctaaag aagatagaac aaaccatggt acaactcgtg
    22321 ctttattaaa caatatggta caaggtgttt ctcaaggata cgtaaaagta cttgaacttg
    22381 ttggtgtagg ttaccgtgct caaatgcaag gtaaagactt aatccttaac gttggttatt
    22441 ctcacccagt agaaattaaa gctgaagaaa acattacttt ctcagttgag aaaaacacag
    22501 tcgttaaagt tgaaggtatt tcaaaagaac aagttggagc attagcatct aacatccgtt
    22561 cagtaagacc tccagagcct tacaaaggta aaggtattcg ttaccaaggt gaatacgttc
    22621 gccgtaaaga aggtaaaact ggtaaataat agataaactc taaagaaagg agtattgaaa
    22681 tgatcagtaa aattgataaa aataaagtgc gtttaaaaag acatgctcgt gttcgtacta
    22741 acttatcagg tacagctgaa aagccacgtt taaacgtata tcgttcaaac aagcatatct
    22801 acgctcaaat tattgatgat aataaaggcg taacattagc tcaagcttct tcaaaagaca
    22861 gcgacattgc tactacagca actaaagttg aattagcaac taaagtcggt gaagcaattg
    22921 ctaaaaaagc tgctgacaaa ggcattaaag aaatcgtatt tgaccgtgga ggatatttat
    22981 atcacggacg tgttaaagca ttagctgaag cagcaagaga aagcggatta gaattttaat
    23041 ttaaaggagg gacaaataca tggctcgtag agaagaagag acgaaagaat ttgaagaacg
    23101 cgttgttaca atcaaccgtg tagcaaaagt tgtaaaaggt ggtcgtcgtt tccgtttcac
    23161 tgcattagtt gtagttggag acaaaaatgg tcgtgtaggt ttcggtactg gtaaagctca
    23221 agaggtacca gaagcaatca aaaaagctgt tgaagcagct aaaaaagatt tagtagttgt
    23281 tccacgtgtt gaaggtacaa ctccacacac aattactggc cgttacggtt caggaagcgt
    23341 atttatgaaa ccggctgcac ctggtacagg agttatcgct ggtggtcctg ttcgtgccgt
    23401 acttgaatta gcaggtatca ctgatatctt aagtaaatca ttaggatcaa acacaccaat
    23461 caacatggtt cgtgctacaa tcgatggttt acaaaacctt aaaaatgctg aagatgttgc
    23521 gaaattacgt ggcaaaacag tagaagaatt atacaattaa ggagggaaaa ctagttatgg
    23581 ctaaattaca aattaccctc actcgtagtg ttattggtcg tcctgaaaca caacgtaaaa
    23641 ctgttgaagc tttaggtctt aaaaagacta acagttcagt agttgttgaa gataaccctg
    23701 ctattcgtgg gcaaatcaac aaagttaagc acttagtaac agtagaagaa aaataatcaa
    23761 ggaggtgccg aaatgaaatt acatgagtta aaaccggcag aaggttcacg taaagaacgc
    23821 aatcgtgttg gacgtggtgt tgcgacaggt aacggtaaaa caagtggtcg cggacacaaa
    23881 ggtcaaaaag ctcgttcagg cggtggtgta agaccaggat ttgaaggtgg tcaattacca
    23941 ttattccgtc gtttaccaaa acgtggtttt actaacataa atcgtaaaga atatgctatt
    24001 gttaacttag accaacttaa taaatttgaa gatggtactg aagtaactcc agctttatta
    24061 gtagaatctg gtgttgttaa gaatgaaaaa tctggtatca aaatactagg taatggttca
    24121 cttgataaga aattgacagt gaaagctcat aaattctcag cttcagcagc agaagctatt
    24181 gatgctaaag gtggagcaca cgaggtgatc taatgattca aacccttgtg aacttcttta
    24241 gaacaaaaga ggttcgtaac aagattttct tcacactagc aatgttagta atttttaaaa
    24301 tagggactta tataccagct ccaggagtaa atcctgcagc ttttgataat ccccaaggtt
    24361 ctcaaggtgc cactgagtta ttaaatactt ttggtggcgg agccttgaaa cgattttcta
    24421 tttttgcaat gggtattgta ccctacatca ctgcatcaat cgtaatgcaa ttattacaaa
    24481 tggatattgt ccctaaattc tcagaatggg caaaacaagg tgaagtaggt agaagaaagt
    24541 taaataacgt tactcgttat ttagcaattt ctttagcatt tatccaatct ataggtatgg
    24601 cattccaatt taataattat ctcaaaggtg cgctgattat caatcagtca attatgagtt
    24661 atttattaat agcactagtt ttgacagcag gaactgcttt cttaatatgg cttggtgatc
    24721 aaatcactca gttcggtgtt ggtaatggta tttctattat catatttgcg ggtattttat
    24781 caacgttgcc agccagtcta attcaatttg gacaaactgc gttcgttggg caagaagata
    24841 catcattagc atggttgaaa gtattaggac ttttagtgtc actgatacta ttaactgtag
    24901 gtgctattta tgtacttgaa gccgttcgta aaattccgat tcaatatgct aagaaacaaa
    24961 ctgctcaaag attaggttca caggcaactt atctaccttt aaaagtgaac tcagccggtg
    25021 ttattcctgt aatctttgca atggcgttct tcttactgcc tagaacatta acgttgttct
    25081 atccagataa agaatgggca caaaacattg cgaatgctgc aaatccttca agtaatgttg
    25141 gtatggttgt atatattgta ctaattatac tattcacata cttctatgca tttgtacaag
    25201 ttaatccgga aaaaatggct gataacctta agaaacaagg tagctatgtt ccaggtatta
    25261 gacctggtga acaaaccaaa aaatatatta ctaaagtttt atatcgttta acttttgtag
    25321 gttctatctt cttagccgtt atttcaattc taccgatatt agcaactaag tttatgggat
    25381 taccacaatc aattcagatt ggtggtacaa gtttacttat cgttattggt gtagcgattg
    25441 aaacaatgaa atctttagaa gctcaagtat ctcaaaaaga atataaaggc tttggtggta
    25501 gataatttta ggagggcact tatgaatatc attttgatgg gtttacctgg cgcaggtaaa
    25561 ggaactcaag caagtgaaat tgtcaagaaa ttcccaatac cccacatttc aactggtgac
    25621 atgttcagaa aagctataaa agaagaaact gaattaggta aagaagctaa gtcttatatg
    25681 gaccgtggcg aattagttcc tgatgaagtg actgtaggta tcgttaagga aagaatttct
    25741 gaagacgatg caaaaaaagg ctttttatta gatggcttcc caagaacaat cgagcaagct
    25801 gaggcattaa ataatattat gtctgagctt gacagaaaca ttgatgctgt catcaatatc
    25861 gaagttccgg aagaagaatt aatgaaccgt cttacaggtc gtcgaatctg tgagtcatgt
    25921 ggtacaacgt atcatcttgt atttaatcct ccgaaggtcg aaggtatttg tgatatcgat
    25981 ggtggtaaat tgtatcaacg agaagatgat aatcctgaaa cggtagctaa tcgtttgagt
    26041 gttaatatta aacaatctaa gcctatttta gatttctatg atcaaaaagg tgtattgaaa
    26101 aatattgatg gttcaaaaga tattagcgac gttaccaaag atgtcattga tattttagat
    26161 catttgtaat agatcaactt ttagcggtct attttaagtg aggcatgatt taatcatatt
    26221 agatagatga gagtggcaat taacgataat taattcccgt aatttttgtt taacttataa
    26281 aattaatcac tgatagattc gtaacaataa agttgtctat tgacgattac cttactattg
    26341 caaaaagggg gaagttaatc aatggctaaa caagatgtaa ttgaattaga aggtactgta
    26401 ttagatactt taccgaacgc aatgtttaaa gtagaattag aaaatggtca tgagatttta
    26461 gctcacgtaa gtggtaaaat cagaatgaat tacattcgta ttctacctgg cgacaaagta
    26521 actgttgaga tgtctccgta cgatttaaca cgcggaagaa ttacttatcg ttataaataa
    26581 tcgtcactcc ataatatagg gaggtataaa aatgaaagta agaccatcag taaaacctat
    26641 ttgcgaaaaa tgtaaagtca ttaaacgtaa aggtaaagta atggtaattt gtgaaaatcc
    26701 aaaacacaaa caaagacaag gttaataaaa gagaggtgta aattaatatg gcacgtattg
    26761 caggagtaga tattccacgt gaaaaacgcg tagttatctc attaacttat atatacggta
    26821 tcggtacgtc aactgctcaa aaaattcttg aagaagctaa cgtatcagct gatactcgtg
    26881 tgaaagattt aactgatgac gaattaggtc gcatccgtga agttgtagac ggttataaag
    26941 tcgaaggtga cttacgtcgt gaaactaact taaatatcaa acgtttaatg gaaatttcat
    27001 cataccgtgg tatccgtcac cgtcgtggtt taccagttcg tggtcaaaaa acgaaaaaca
    27061 acgcgcgtac tcgtaaagga ccagttaaaa cggtagctaa caagaaaaaa taataggtaa
    27121 aggaggcaaa ttttaaatgg cacgtaaaca agtatctcgt aaacgtagag tgaaaaagaa
    27181 tattgaaaat ggtgtagcac acatccgttc aacattcaac aacactattg taactatcac
    27241 tgatgagttc ggtaatgctt tatcatggtc atcagctggt gcattaggat tcaaaggatc
    27301 taaaaaatca acaccatttg cagcacaaat ggcttctgaa actgcatcta aatcagctat
    27361 ggagcatggt ttaaaaacag ttgaagtaac agttaaagga cctggtccag gtcgtgaatc
    27421 agctattcgt gcattacaat ctgcaggttt agaagtaact gcgatcagag acgttactcc
    27481 agtacctcat aacggttgtc gtccaccaaa acgtcgtcgt gtataattta tgatggtatt
    27541 gttacaggtc actgagcaaa cattttaaat taagtcgacg tatataagga ggatatttaa
    27601 atgatagaaa tcgaaaaacc tagaattgag acaattgaaa ttagtgaaga tgctaaattc
    27661 ggtaagttcg ttgttgaacc actagaacgt ggctacggta ctacactagg aaactcctta
    27721 cgtcgtatcc tactatcttc attaccaggt gcagccgtta agtatattga aattgaggga
    27781 gttttacatg aattctcagc agtagacaat gtagttgaag atgtttctac aattattatg
    27841 aacattaaac aattagcatt gaaaatttac tctgaagaag ataaaacttt agaaattgat
    27901 gtacgtgatg aaggcgaagt aacagcaagc gacattacac atgatagtga tgttgaaatt
    27961 ttaaacccag agcttaaaat tgcaacagta tctaaaggtg gtcacttaaa aattcgtcta
    28021 gttgctaaca agggtagagg ttacgcatta gcagaacaaa ataatactag tgatttacca
    28081 attggtgtaa tccctgttga ttcattgtat tcacctgttg aacgtgtgaa ctatactgtt
    28141 gaaaatacac gtgtaggtca aagcagtgat tttgataaat taacattgga tgtttggact
    28201 aatggttcaa tcacaccaca agaatcagtt tcattagcag caaaaataat gactgaacac
    28261 ttgaatatct tcgttggtct tactgatgaa gcgcaaaacg ctgaaatcat gattgaaaaa
    28321 gaagaagatc aaaaagaaaa agtattagaa atgtctattg aagaattaga cttatctgta
    28381 cgttcatata actgcttaaa acgcgcagga atcaattctg ttcaagagtt agctgacaaa
    28441 tctgaagctg acatgatgaa agtgcgtaat ttaggtcgta aatctttaga agaagttaaa
    28501 tacaaattag aagatttagg attaggatta agaaaagaag attgataaag gaggttaact
    28561 catgggttac agaaaattag gtcgtacttc tgatcaacgt aaagctatgt tacgtgactt
    28621 agctacatca cttattatta gtgaacgtat tgaaactaca gaagctcgtg caaaagaagt
    28681 tcgcagtgtt gttgagaaat taatcacttt aggtaaaaaa ggagatttag cttctcgtcg
    28741 taatgcagct aaaactttac gtaatgttga aatcttaaac gaagatgaaa ctacacaaac
    28801 tgcacttcaa aaattatttg gtgaaatcgc agagcgttac acagaacgtc aaggtggtta
    28861 cactcgtatc cttaaacaag gccctcgtcg cggtgacggt gctgaatcag taattatcga
    28921 attagtataa gaatttatat aaatacacta actactttta tataaagcaa atgagtgcaa
    28981 cgataatgtt tgccacatac aaatattgtc tagctcagag taccccatca actaaataaa
    29041 gatttaaagc gtgaactcaa caaacttatg atggggtgcg cgctttttta ttgaataaaa
    29101 tatcggactt atgaacagaa atgtttttaa gtccgatttc tttatattta cttttttgag
    29161 tgcgaatcta aaaatatgat gaagctagtt tgatcaattt cactagaact gaatgaagtg
    29221 aaagtattat cgttgaatca aacgcgacca cccctatatt agtaaatcat ttattttatt
    29281 aacgtttata cataattgtt agagaggaat aaaagtcctt atcattagtt gttaaacaac
    29341 actttaaaga ctagaaatga gtcagaaaat actagtaata tatagagaac ccagtcaatg
    29401 tcatatacag cgatttataa atattgtaaa atatgataga tacacgaatc gtaaaggaga
    29461 gagacaagtg gaggataaga attcagttat tgtatttaaa aatgtttcat ttcaatatca
    29521 aagtgatgca tccttcacat tgaaagatgt ttcttttaat atacctaaag gtcagtggac
    29581 atctattgtt ggtcataacg gttctggaaa atctacaatt gccaagttaa tgattggcat
    29641 agagaaagtt aaatctggag aaatttttta taataatcaa gctataactg atgataattt
    29701 tgaaaagtta agaaaagaca taggaattgt atttcagaat ccggataatc aatttgttgg
    29761 ttcaattgta aaatacgatg tggcatttgg actcgaaaat catgcggttc catatgacga
    29821 aatgcataga agagtcagcg aagcacttaa acaagttgat atgttagaac gtgcagatta
    29881 tgaacctaat gcattatcgg ggggacagaa gcagcgtgtg gctatagcaa gtgtattagc
    29941 acttaacccc tctgtcatta tattagatga ggcgacttct atgttagatc ctgatgcacg
    30001 tcaaaattta ttggatttag tgagaaaagt taaatcagaa cataatatta caatcatttc
    30061 tattacgcat gatttatctg aggcgatgga agcagatcat gttatcgtta tgaataaagg
    30121 gactgtctat aaagaaggca cagcgactga aattttcgac aatgcagaag agttaacaag
    30181 aataggtcta gatttgccat tcccaatcaa aataaatcaa atgctgggac accaaacatc
    30241 attcttaact tatgaagggc tggtggatca actatgacta tacggtttga caatgtaagt
    30301 tatacctatc aaaaagggac accatatcag catcaagcta ttcatgatgt taatacagaa
    30361 tttgaacaag gtaaatatta cgccatcgtt ggacaaacgg gtagtggtaa atcaacgttg
    30421 atacaaaata ttaatgcgct gttaaagccg actactggga cagttacagt tgatgacatt
    30481 actattacac ataagaccaa agataaatat attagacctg taagaaaaag aattggaatg
    30541 gtatttcaat ttcccgaatc tcaattattt gaggacacag tagagcgtga aatgatattt
    30601 ggacctaaaa actttaaaat gaatttagat gaagccaaaa actatgccca tcgtctgttg
    30661 atggatttag gcttttcaag agatgtaatg tcacaatcac catttcaaat gtcaggtgga
    30721 caaatgcgta aaatagcgat tgtatcgata ttggcaatga atcctgatat tatcgtggtt
    30781 gatgaaccta cagcaggact tgatccacaa agtaaacgac aagtaatgag attactaaag
    30841 tcactacaaa cagatgaaaa taaggcaatt atcctaattt cacatgatat gaatgaagtc
    30901 gcgcgttatg cagatgaagt catcgttatg aaagaaggta gtatagtgtc gcaaacatca
    30961 ccaaaagagc tcttcaaaga taaaaaaaaa ttggcggatt ggcatattgg tttgccagaa
    31021 attgttcagt tacaatatga ctttgaacaa aaatatcaaa caaaattaaa agatattgcc
    31081 ttaactgaag aagcatttgt aagcttgtat aaggagtggc aacatgaaaa ataaattgat
    31141 tatagggcga tatttaccaa ttaattcttt tgttcatcat cttgatccaa gagcaaagtt
    31201 aatgtttgtt tttcttttta ttatattaat atttttctgt cattcaccat taacatatct
    31261 ttgggtgttt gcacttatct tattcattat gagattagca aaaattcaat tatggttcct
    31321 aatcaaaggt ttaacaccaa tatttttctt tttaatcttc acattaatga tgcatatatt
    31381 tttaactaaa ggtggatatg tattagttga atggcatggc atcacgattg aaactaatgg
    31441 tattttagaa gggctatata tatcactgcg tttaattggg attgtaatga ttgcaacaat
    31501 tatgacacta tctacaagtc caattgattt aacagatgcg tttgaaagat tacttgcacc
    31561 actaaagatg tttaaattac cagttcatca attaagtatg ataatgtcca ttgcgttacg
    31621 attcatcccg acgttaatgg atgagttaga taaaatcatt ttggcgcaaa agtcgcgtgg
    31681 ttctgaaata agttcaggga acattgcaac acgtattaaa tcatttattc cattactagt
    31741 gccactattc atctcagctt tccaacgcgc cgaagaatta gcggtcgcaa tggaagttag
    31801 gggttatgat gccaatgtta agagaacgag ctacagacag cttaaatggc aattgagaga
    31861 tacgatatct ttaaccatga ttatcccaat tgcaattatt ttattcgtat taaaatattc
    31921 aggagtgtaa attcatgcgt atattagtag aaattgcgta tcaaggaaat aattttctag
    31981 gttttcaaat tcaacaaaat ggtcgaacgg tacagcaaca atttgaaaag ctattacaac
    32041 gcatgcataa aagacatgtg agaatccatc cttcaagtag gactgataga ggtgtacatg
    32101 cgatacagca atactttcat ttcgacacag agctgaatat acctatgtca caatggcaat
    32161 atgcaatgaa ccgaacgtta cctgatgata tttatgtcaa caatgtagtt acagtcgatg
    32221 acgattttca ttgtcgttat gattgtgtag gtaaacgtta tcgctacaaa gtatatcaag
    32281 cacaacaccg cgatccattt caaagtggtt tgaaaacatt tattcctgaa acgttggatt
    32341 taggtaaaat gaacagagcg gcgcaacaat tcataggtac acatgatttt acgggatttt
    32401 gttcgcaaaa aactgaagta gaaagcaaag ttagaacatt ataccaaagt gaaatcgtaa
    32461 agacagatga tggctttgat tatattgtca ctggatctgg atttttatat aacatggtgc
    32521 gtgtcttagt tgcattttta atagaagttg gaaaagggcg acatgaagta tctgatgtac
    32581 caaaacttct tgaatcaaag aatcgtaaga atgtaccttt cactgcaccg gctgaaggat
    32641 tatatttaga gaaaatttat ttagatgaaa atgagttatt aaaagacttt ggtaacgata
    32701 tcaaaataca tcgtaaaaaa tcattacaaa atgactgatt gccattgaca aaaagcttgt
    32761 gaaattataa gattattaac ggtattgttt tatatccacc ccacgataag ccccggaaac
    32821 ttattgtgtt acaagatata taagcagaaa cgaacaacag ttaacaaaat aaatgaaatt
    32881 aaacgtttta aaaatgaaac aaatgaaatc atctattagg ttatgaaact gtttatagct
    32941 tgaatagaag catttatttt ttaggaggac aattattatg cgtcaaacat ttatggcaaa
    33001 tgaatcaaac attgagcgca aatggtatgt tatcgatgct gaaggccaaa cattaggtcg
    33061 tttatcatca gaagtagcat ctatcttacg cggtaaaaat aaagtaactt acacaccaca
    33121 cgttgatact ggtgattatg taatcgttat taatgcatca aaaatcgaat ttactggtaa
    33181 caaagaaact gacaaagttt actaccgtca ctcaaatcac ccaggtggta tcaaatcaat
    33241 cactgctggt gaattaagaa gaactaaccc agaacgttta attgaaaact caattaaagg
    33301 tatgttacca agcactcgtt taggcgaaaa acaaggtaaa aaattatttg tatatggtgg
    33361 cgctgaacat ccacacgctg cacaacaacc agaaaactac gaattacgtg gttaattaga
    33421 aggaggaaat gactttggca caagttgaat atagaggcac aggccgtcgt aaaaactcag
    33481 tagcacgtgt acgtttagta ccaggtgaag gtaacatcac agttaataac cgtgacgtac
    33541 gcgaatactt accattcgaa tcattaattt tagacttaaa ccaaccattt gatgtaactg
    33601 aaactaaagg taactatgat gttttagtta acgttcatgg tggtggtttc actggacaag
    33661 ctcaagctat ccgtcacgga atcgctcgtg cattattaga agcagatcct gaatacagag
    33721 gttctttaaa acgcgctgga ttacttactc gtgacccacg tatgaaagaa cgtaaaaaac
    33781 caggtcttaa agcagctcgt cgttcacctc aattctcaaa acgttaattg tcggacgata
    33841 tatacaaaac acctcgatat tatgtcgagg tgtttttttg cgtttttgcg gcgaatatgg
    33901 aatgtgtaga atataaatga atttttacct tcccaccata aaagatgaag aaccatgaaa
    33961 tgtggagaac aataaatagt tgatattctg ttattttttt ggaagtgaag tgatttgaat
    34021 actttactct aaacgataaa aggttaaaaa aacaacaaaa agaaaatatt actgtcataa
    34081 ttggaaatac accgtattct gctggtcaat ctagtgtaaa taatgataat aaaaaaatga
    34141 atatgaaaaa ttaaatgcaa aaatagcaaa tacctatgca aatttttcaa cagcaaattt
    34201 aaaaaaatca ttatatgatt cgtatataca ggcttttcga tgggcaactg atagagttgg
    34261 aaaaaaggta ttataggatt tgtaacaaat ggatcattta ttgatagtca aagcactgat
    34321 ggatttagaa aagttttata cgatgaattt aactatctgt acataattaa tttgagaggc
    34381 gaccaacgaa cacagggtga aaaatcaaga aaagaaggtg gaaaaatatt tggatcagga
    34441 agtagagcac cgatagctat atctatttta gttaaagatg gaagttacaa tcacgatatc
    34501 tattataatg acattggtga atatttaact agagagcaaa aattagatac tttaatgaaa
    34561 catcaatcaa ttgtgaattt gaaaagtttg aatgttttac ccgataaaaa caatgattgg
    34621 attaatcaac gagacataaa ctatgagaat tatttaccaa tgtatgattc caaggatatt
    34681 gaaaattcta tatatttaga tcaatttaat ggagtaaatt cagcaagaga taattgggta
    34741 acaaattttt cgaatgaaaa agcattagta aatgcaaaat tacttgtaga taactataat
    34801 tctgaaatag atagattaat tgatatctta gattcaagag aacgtataaa tttagtgaac
    34861 aaagatgaaa catttattag ttggacacga ggacttaccc aaaaattttc taaaggaaaa
    34921 aatatttcaa ttaatccaga gagaattgtt aaatttatgc atagaccatt tacaaaaaaa
    34981 tggatagtgt acgacaaaaa tattatggaa atgccaagta gatattataa tataatggag
    35041 aatactggac aggtgattta tatacaagga cagggaatga ataaagaatt ctcggctatg
    35101 atcacggaca tattacctaa tttccaattt attggtaatg gcaaaggctt tgctacatac
    35161 aaaggtaaag atagtttgag gcttgtagat aatatttcta attcttttaa aaagaaaata
    35221 aatcttaatt cagaagagat agtatattat atatatgcaa ttttgcatca taaatattat
    35281 gtgaacaaat attcaagtga tttgagtaaa ggttttccgc gaataccgat attaaaggat
    35341 gtatatggat ttgttgaaat tggtagggag ttagtggaat tacatttaaa ttatgaaaaa
    35401 caactcaatt gggatggtgt agaaataatt tataataata tgaatccgaa ttataaagtt
    35461 gaaaaatgaa gcaccctaaa aagggcgtgt tggatacagt aatatacaat accgatatta
    35521 cagttactaa cattccaaat aaagtatata attatattgt gaatggcaag cctgctattg
    35581 aatggattat agatcagtac aacgtaagca tcgacaagaa atctggaata ttagatgatc
    35641 ctaatgaatt tagtgaagat ccgaattaca ttttaaatct gcttttatca gttattactg
    35701 taagtatgaa aactcttgga ttaatagata aattaccaga ttttgaagta ttagattaag
    35761 gagaggatat attgacaaat actatcaaaa aaataattta ttttgatgaa ctatcagcaa
    35821 tggatttatt acagatagag aagaaaggta accttactaa aacagttcag ttgatgcatg
    35881 atgaaaaagg tagtgcaagt ggtgaatcta aagtgaacat ggaatttggt aagcaaagcg
    35941 ctgtaaaaca aatttttgat tctttacttg ggatgtcagc gagtgcttcg gctagtgcag
    36001 gtataaatgg aaatataact ggagagcgag tagccaaaac aattttagag aatacattaa
    36061 tatatgattt tttggataca gtagagttta gaaagaaaaa aactattatt gatatttcag
    36121 aggggtttaa actaaatatt caacaagata cgatgacata tttcgcgact attaccccca
    36181 tagcaagtat gtttgaagga tctaaacatc tagacgagga ggagctatca tttgatgttt
    36241 caaaaatgaa tgaagcgatt agaagtatta aaggctattt tgaattaata ggaacaagag
    36301 ggaatgaaaa cagagttttt agatttaata tagaatcttt taaaaataac tatagaatac
    36361 aagatttaaa atttatgaat ttaacactct actcagtact tgttggtgaa ataagcaaag
    36421 aaaatctaaa ttacgataaa gagtttgatc taaatcaagg tgccaaacct agtggaattg
    36481 gaaagtttaa gggaattaga tcagaagcaa ctaaagaaga taaatatcaa gtgtatgatg
    36541 ttattcttgc aggagtgaaa taaatggaaa ttcatgttta tacgggaccg ctttgtggat
    36601 ttaatgaata tattaattct gaagttaaag aatatcaccg cttcatggaa ttaatcagag
    36661 aatacaatat catagtgcga gcaagtgata catttggaac tcatgatgtt gatggtattt
    36721 ttgatactga gatagaaaat cttgtgatat tttcaaacga ctttgcttct gtaacaactc
    36781 atgttattac taattttata aatatcgtta ctttaggtag aaatattaag aaaatctata
    36841 ttcagaatcc tccaaaaaga gtatttcaat cactaaatag ttttcacgat tctgaaataa
    36901 tagaaataaa ctatgaatat acaacattaa aaaaagatga tgtaataaat ttttataaag
    36961 agcagatggc tggttctaaa ataataggtc aaacagacgc taaaaaacaa atgagcatag
    37021 atttatataa acatacttcc ttagaaaata ataaaccgac cgtaatgctt tactatggtc
    37081 catcaggtgt tggaaaaaca gaattagcca aagaaatagc aaaaaaatat aaaggtaata
    37141 tcactagaat acaattttct atgatgcaga atgatgaatc ttttaattat atctttggag
    37201 atgaacatgc caaagtatca ttgtcaaaag atttagtaga tcgtgaaact aatgttgttt
    37261 taattgatga atttgataag gtattaccaa tctattacaa tgttttctat caaatgtttg
    37321 atgaaggtat tattgaagat gccaattata aagtagacgt ttcaaattgt attttcattc
    37381 taacatctaa ttttaatagt attgaagaag caatagttaa tattggagaa ccggtatttt
    37441 ctagaataga ttcctgtatt aaatttttat atttaacaca ggaagaaaag cagaaag
//
LOCUS       DALAGD010000011        37006 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.011, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000011 DALAGD010000000
VERSION     DALAGD010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 37006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37006
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.011"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(<1..1179)
                     /locus_tag="O2701_000417"
     CDS             complement(<1..1179)
                     /locus_tag="O2701_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1181 family transposase"
                     /protein_id="HCZ4292330.1"
                     /translation="MCNDTLELLRIKDENIKYINQEIDVIIKGKKATVVNAVLTYKPS
                     ACYCCGVKNEGQIHKHGKRVSRITLLKTQGYNTYLNLAKQRFKCLECNGTFTAKTSIV
                     DESCFISRCVTQKVIEEATKVKTEIDTAEDNCISPSTVSRIRTKAANSLRIKPFNCLP
                     EHIAMDEFKSVKNVTGSMSFIFIDNDTHDVIDILENRTTRFLRAYFERFDLKNRQQVK
                     TVTIDMYEPYVRLFRDLFPNAAIIFDRFHIVQHLNRELNKYRVQVMNEYRNKKGPDYT
                     IFKNNWKVLLMDTSKTIFSKYRWNKSFKAYKRSSDIVEFMLSKDDILRHSYELVQGLR
                     KDLRLCNWPKFINRLNSVSKKSVSKGVWKAVKYYRKHQRMLRNTIYYPAFNNGAIEGI
                     N"
     gene            1405..2313
                     /gene="rocF"
                     /locus_tag="O2701_000418"
     CDS             1405..2313
                     /gene="rocF"
                     /locus_tag="O2701_000418"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HCZ4292331.1"
                     /translation="MTKTKAIDIIGAPSTFGQRKLGVDLGPTAIRYAGLISRLKQLDL
                     DVYDKGDIKVPAVNIEKFHSEQKGLRNYDEIIDVNQKLNKEVSASIENNRFPLVLGGD
                     HSIAVGSVSAISKHYNNLGVIWYDAHGDLNIPEESPSGNIHGMPLRILTGEGPKELLE
                     LNSNVIKPENIVLIGMRDLDKGERQFIKDHNIKTFTMSDIDKLGIKEVIENTIEYLKS
                     RNVDGVHLSLDVDALDPLETPGTGTRVLGGLSYRESHFALELLHQSHLISSMDLVEVN
                     PLIDSNNHTAEQAVSLVGTFFGETLL"
     gene            2502..3311
                     /locus_tag="O2701_000419"
     CDS             2502..3311
                     /locus_tag="O2701_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HCZ4292332.1"
                     /translation="MDFSNFFQNLSTLKIVTSILDLLIVWYVLYLLITVFKGTKAIQL
                     LKGILVIVIGQQISMILNLTATSKLFDIVIQWGVLALIVIFQPEIRRALEQLGRGSFL
                     KRYTSNTYSKDEEKLIQSVSKAVQYMAKRRIGALIVFEKETGLQDYIETGIAMDSNIS
                     QELLINVFIPNTPLHDGAMIIQGTKIAAAASYLPLSDSPKISKSLGTRHRAAVGISEV
                     SDAFTVIVSEETGDISVTFDGKLRRDISNEIFEELLAEHWFGTRFQKKGVK"
     gene            3313..4245
                     /locus_tag="O2701_000420"
     CDS             3313..4245
                     /locus_tag="O2701_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HCZ4292333.1"
                     /translation="MLESKWGLRFIAFLLALFFFLSVNNVFGNIFNTGNLGQKSSKTI
                     QDVPVEILYNTKDLHLTKAPETVNVTISGPQSKIIKIENPEDLRVVIDLSNAKAGKYQ
                     EKYQVKGLADDIHYSVKPKLANITLENKVTKKMTVQPDVSQSDIDPLYKITKQEVSPQ
                     TVKVTGGEEQLNDIAYLKATFKTNKKINGDTKDVAEVTAFDKKLNKLNVSIQPNEVNL
                     QVKVEPFSKKVKVNVKQKGSLADDKELSSIDLEDKEIEIFGSRDDLQNISEVDAEVDL
                     DGISESTEKTVKINLPEHVTKAQPSETKAYINVK"
     gene            4272..5627
                     /locus_tag="O2701_000421"
     CDS             4272..5627
                     /locus_tag="O2701_000421"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCZ4292334.1"
                     /translation="MGKYFGTDGVRGVANQELTPELAFKLGRYGGYVLAHNKGEKHPR
                     VLVGRDTRVSGEMLESALIAGLISIGAEVMRLGIISTPGVAYLTRDMGAELGVMISAS
                     HNPVADNGIKFFGSDGFKLSDEQENEIEALLDQENPELPRPVGNDIVHYSDYFEGAQK
                     YLSYLKSTVDVNFEGLKIALDGANGSTSSLAPFLFGDLEADTETIGCSPDGYNINEKC
                     GSTHPEKLAEKVVETESDFGLAFDGDGDRIIAVDENGQIVDGDQIMFIIGQEMHKNQE
                     LNNDMIVSTVMSNLGFYKALEQEGIKSNKTKVGDRYVVEEMRRGNYNLGGEQSGHIVM
                     MDYNTTGDGLLTGIQLASVIKMTGKSLSELAGQMKKYPQSLINVRVTDKYRVEENVDV
                     KEVMTKVEVEMNGEGRILVRPSGTEPLVRVMVEAATDEDAERFAQQIADVVQDKMGLD
                     K"
     gene            5904..13340
                     /locus_tag="O2701_000422"
                     /pseudo
     CDS             5904..13340
                     /locus_tag="O2701_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
     gene            complement(13659..14765)
                     /locus_tag="O2701_000423"
     CDS             complement(13659..14765)
                     /locus_tag="O2701_000423"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCZ4292335.1"
                     /translation="MKAVHFGAGNIGRGFIGYILADNNVKVTFADVNEEIINALAHDH
                     QYDVILADESKTTTRVNNVDAINSMQPSEALKQAILEADIITTAVGVNILPIIAKSFA
                     PFLKEKTNHVNIVACENAIMATDTLKKAVLDITGPLGNNIHFANSAVDRIVPLQKNEN
                     ILDVMVEPFYEWVVEKDAWYGPELNHIKYVDDLTPYIERKLLTVNTGHAYLAYAGKFA
                     GKATVLDAVEDSSIEAGLRRVLAETSQYITNEFDFTEAEQAAYVEKIIDRFNNSYLSD
                     EVTRVGRGTLRKIGPKDRIIKPLTYLYNKDLERTGLLNTAALLLKYDDTADQETVEKN
                     NYIKEHGLKAFLSEYAKVDDGLADEIIEAYNSLS"
     gene            complement(14765..15199)
                     /locus_tag="O2701_000424"
     CDS             complement(14765..15199)
                     /locus_tag="O2701_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCZ4292336.1"
                     /translation="MSELFSNDNIFLNVNVNSQNEAIEKAGKALVDSGAVTDAYIQAM
                     KDREQVVSTFMGNGLAIPHGTDEAKTNVIHSGLTLLQIPEGVDWDGEVVKVVVGIAGK
                     DGEHLDLLSKIAITFSEEENVDRIVQAKSAEEIKQVFEEADA"
     gene            complement(15211..17343)
                     /locus_tag="O2701_000425"
     CDS             complement(15211..17343)
                     /locus_tag="O2701_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCZ4292337.1"
                     /translation="MLLSTREKEMIALLIKYHGQYITIHDIAQQLAVSSRTIHRELKG
                     VEAYLTSFSLTLERANKKGLRIAGTDSDLNDLKQSIAQHQTIDLSVEEQKVIIIYALI
                     QAKEPVKQYSLAQEIGVSVQTLAKMLDDLELDLNKYQLSLSRKRGEGIYLVGTESKKR
                     EFLSQLMVNNLNSTSVYSVIENHFVFHSLNQIHKDFVDLERIFNVERLLMDYLSALPY
                     QLTESSYLTLTVHIVLSISRIKNGEYVALNDDIYDSVQNTFEHKVASELADKLGQIYD
                     VTFNQAEIAFITIHLRGAKRKNLNDTSLNNRCEENKIKAFVNKVEMISGMTFADLDTL
                     VDGLTLHLNPAINRLQANIETYNPLTDMIKFKYPRLFENVRLALNDCWPDLIFPESEI
                     AFIVLHFGGSIKNQGNRFLNILVVCSSGMGTSRLLSTRLDQVFSEIERITQASVSDLK
                     SLDLSQYDGIISTVNLDIDSPYLTVNPLLPDSDISYVAQFLNTKSTFQETHDKSSNMI
                     DKDDVHVETKDVDGNTSFENEQTSYLTSVFEKHLSDEKSEQLLHHMRSGLTLLDSVKI
                     VSTEVKQWQTYIADYLYQCDVINDPTSFAELLEQRLIDNPGWILSPYPVAIPHLRDNM
                     IKHPMILITVLEEPLTLPSIQNDNQTIKYMISMFISDNDFMASLVSDLSEFLSLKLES
                     IDTFMENPQELETLLRNKFLERIKKQFI"
     gene            complement(17378..18916)
                     /locus_tag="O2701_000426"
     CDS             complement(17378..18916)
                     /locus_tag="O2701_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCZ4292338.1"
                     /translation="MSQTEEKKGIGRRVQAFGSFLSSMIMPNIGAFIAWGFIAAIFID
                     NGWLPNKDLATLAGPMITYLIPLLIAFSGGRLIYDLRGGIIAATATMGVIVALPDTPM
                     LLGAMIMGPLVGWLMKKTDQLIQPRTPQGFEMLFNNFSAGILGFIMTIAGFKILAPLM
                     KFIMHILSVAVEALVHAHLLPLVSILVEPAKIVFLNNAINHGVFTPLGADQAAKAGQS
                     ILYTIESNPGPGLGILLAYMIFGKGTAKATSYGAGIIHFLGGIHEIYFPYVLMRPLLF
                     IAVILGGMTGVATYQATGFGFKSPASPGSFIVYCLNAPRGEFLHMLLGVFLAALVSFV
                     VAALIMKFTREPKQDLEAATAQMENTKGKKSSVASKLVSSDKNVNTEENASGNVSETS
                     SSDDDPEALLDNYNTEDVDAHNYNNINHVIFACDAGMGSSAMGASMLRNKFKKAGIND
                     ITVTNTAINQLPKDAQLVITQKKLTDRAIKQTPNAIHISVDNFLNSPRYEELLNNLKK
                     DDQA"
     gene            19597..21402
                     /gene="glmS"
                     /locus_tag="O2701_000427"
     CDS             19597..21402
                     /gene="glmS"
                     /locus_tag="O2701_000427"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCZ4292339.1"
                     /translation="MCGIVGYIGYDNAKELLLKGLEKLEYRGYDSAGIAVVNDDNTTV
                     FKEKGRIAELRKVADSSDFDGPVGIGHTRWATHGVPNHENSHPHQSSNGRFTLVHNGV
                     IENYEELKGEYLQGVSFISETDTEVIVQLVEYFSNQGLSTEEAFTKVVSLLHGSYALG
                     LLDAEDKDTIYVAKNKSPLLLGVGEGFNVIASDALAMLQVTSEYKEIHDHEIVIVKKD
                     EVIIKDADGNVVERDSYIAEIDASDAEKGVYAHYMLKEIHEQPAVMRRIIQEYQDAEG
                     NLKIDQDIINDVKEADRIYVIAAGTSYHAGLVGKEFLEKWAGVPTEVHVASEFVYNMP
                     LLSEKPLFVYISQSGETADSRAVLVETNKLGHKSLTITNVAGSTLSREADHTLLLHAG
                     PEIAVASTKAYTAQIAVLSILSQIVAKEHGREADIDLLRELAKVTTAIEAIVDDAPIM
                     EQIATDFLETTRNAFFIGRTIDYNVSLEGALKLKEISYIQAEGFAGGELKHGTIALIE
                     EGTPVVGLATQEKVNLSIRGNVKEVVARGAHPCIISMEGLEKEGDTYVIPHVHELLTP
                     LVSVVALQLISYYAALHRDLDVDKPRNLAKSVTVE"
     gene            21634..22416
                     /locus_tag="O2701_000428"
     CDS             21634..22416
                     /locus_tag="O2701_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4292340.1"
                     /translation="MLIQLDQIGRMKQGKTILKKISWQIAKGDKWILYGLNGAGKTTL
                     LNILNAYEPATSGTVNLFGKMPGKVGYSAETVRQHIGFVSHSLLEKFQEGERVIDVVI
                     SGAFKSIGVYQDIDDEIRNEAHQLLKLVGMFAKAQQYIGYLSTGEKQRVMIARALMGQ
                     PQVLILDEPAAGLDFIARESLLSILDSLSDSYPTLAMIYVTHFIEEITANFSKILLLK
                     DGQSIQQGAVEDILTSENMSRFFQKNVAVQRWNNRFSMAMLE"
     gene            22484..23341
                     /locus_tag="O2701_000429"
     CDS             22484..23341
                     /locus_tag="O2701_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCZ4292341.1"
                     /translation="MDNVKAIFLDMDGTILHENNQASTYTKDVINQLREKGYKVFLAT
                     GRSHSEIHQLVPQDFAVNGIISSNGTIGEVDGEIIFKHGLSLAQVQQITNLAKRQQIY
                     YEVFPFEGNRVSLKEDETWMRDMIRSQDPINGVSHSEWSSRQDALAGKIDWVTKFPEG
                     EYSKIYLFSSNLEKITAFRDELKQNHVQLQISVSNSSRFNAETMAYQTDKGTGIKEMI
                     AHFGIHQEETLVIGDSDNDRAMFEFGHYTVAMKNARPEIQALTSDVTAYTNEEDGAAK
                     YLAEHFLAE"
     gene            complement(24046..24138)
                     /locus_tag="O2701_000430"
     CDS             complement(24046..24138)
                     /locus_tag="O2701_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292342.1"
                     /translation="MFIFKTTSKSHFHNNVKSLECIKIPINKNR"
     gene            24427..25563
                     /locus_tag="O2701_000431"
     CDS             24427..25563
                     /locus_tag="O2701_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HCZ4292343.1"
                     /translation="MSVNARDLLVLHTNVNRLVGEEIFANKCLANNDVQIMNSIKKLI
                     EAELLTTTNDFEVSIYKKTRPELQSILKSFGIKTTGNKPDLIKRIDDNFHIINNLDLP
                     YVYIPTKKGEEILKKTEYLTSFIQSYGEISLERAYYLVENYIDENCDDKVAEIYKFEF
                     QRKYDNGEFDFNHGYNFELNMLIDHYKRDVKDYDNARKYSNIYLYFGLRDFLKKLMSN
                     YSYYDSKGNIDLNEIQNDLNRFINSSASGMYERLIYNENLSNNIMFELFKKDTQDYSD
                     LEEQLIEKFINYVVSNVKKESRSNTLIELSKILENGYTIDKEEFKKEDDYLSKYIFTD
                     IDYLKKLESKINVAIDIRSGEIHLVLDDDSLDILIQNQKYGNEF"
     gene            25606..26089
                     /locus_tag="O2701_000432"
                     /pseudo
     CDS             25606..26089
                     /locus_tag="O2701_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837446.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            26250..26354
                     /locus_tag="O2701_000433"
     CDS             26250..26354
                     /locus_tag="O2701_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292344.1"
                     /translation="MLLLERTSMSDFEMLMVVLTIIGLVLISTQDHKK"
     gene            complement(26854..27945)
                     /locus_tag="O2701_000434"
     CDS             complement(26854..27945)
                     /locus_tag="O2701_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCZ4292345.1"
                     /translation="MFTYFKSAFKNAKPQLLITLIYALIAFAVIAVVYLLANFQLAKY
                     AQTIAIYSQFGQKPPVDAYLKVIAVLLIAAVVSLFVLVQIFIGITNVMKRAMSHEKVK
                     FTDLFIAFKKGNYLKSVLIGLVSIAMIIVLSLLTSLLYKLFSPVSEMIMNSVQSSYAD
                     STHLIGIAITTQSIIIIVVLLIKAIITWLLLIPIFNFMTSFVESTNDKVKTHLANGFK
                     AMKNGQKTFFKFFIGILLLNLIIILFKTPVGYLISFNTQSLSQSVAENIIRVYTVMTI
                     ILFVVIHAIILMGIVQYYLKRGQKITKDKVKTADKNKKVVTEPKNTKVENDKVTTSVE
                     TKTEKAQDSLNDNTTKTMTSDKPEDNQPK"
     gene            complement(28211..29191)
                     /gene="czrB"
                     /locus_tag="O2701_000435"
     CDS             complement(28211..29191)
                     /gene="czrB"
                     /locus_tag="O2701_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc efflux transporter CzrB"
                     /protein_id="HCZ4292346.1"
                     /translation="MSHSHHHHDHMHSHVTTNNKKVLFISFLIIGLYMFIEIIGGLLA
                     NSLALLSDGIHMFSDTFSLGVALVAFIYAEKNATTTKTFGYKRFEVLAALFNGVTLFV
                     ISILIVFEAIKRFFVPSEVQSKEMLIISIIGLIVNIVVAFFMFKGGDTSHNLNMRGAF
                     LHVIGDLLGSVGAITAAILIWAFGWTIADPIASILVSVIILKSAWGITKSSINILMEG
                     TPSDVDIDEVITTIKKDSRIQSVHDCHVWTISNDMNALSCHVVVDHTLTMKECELLLE
                     NIEHDLLHLNIHHMTIQLETPNHKHDESIICSGTHSHSHNHHAHHHAHVH"
     gene            complement(29193..29513)
                     /gene="czrA"
                     /locus_tag="O2701_000436"
     CDS             complement(29193..29513)
                     /gene="czrA"
                     /locus_tag="O2701_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)-responsive metalloregulatory
                     transcriptional repressor CzrA"
                     /protein_id="HCZ4292347.1"
                     /translation="MSEQYSEINTDTLERVTEIFKALGDYNRIRIMELLSVSEASVGH
                     ISHQLNLSQSNVSHQLKLLKSVHLVKAKRQGQSMIYSLDDIHVATMLKQAIHHANHPK
                     ESGL"
     gene            29665..30330
                     /locus_tag="O2701_000437"
     CDS             29665..30330
                     /locus_tag="O2701_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCZ4292348.1"
                     /translation="MNILVIGANGGVGSLLVQQLAKENVPFTAGVRQSDQLNALKSQG
                     MKAILVDVENDSIETLTETFKPFDKVIFSVGSGGNTGADKTIIVDLDGAVKSMIASKE
                     ANIKHYVMVSTYDSRRQAFDDSGDLKPYTIAKHYADDYLRRSGLNYTILHPGALTNAA
                     GSGKIEAAQYFDGKGEIPREDVATVLKEIVTSNHFNHQEFQIISGDQDIKDALTQFEN
                     ETD"
     gene            30765..31703
                     /locus_tag="O2701_000438"
     CDS             30765..31703
                     /locus_tag="O2701_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HCZ4292349.1"
                     /translation="MPLFLQPILKTKLWGGQRLSEFGYQLDNDTTGECWCVSAHPNGT
                     SEIINGPYQGQTLDRIWSEHRELFGDFPSKDFPLLTKIVDARESLSIHVHPDNSYAYE
                     HENGQYGKSECWYIIDAEEDAEIVIGTLAESREEVANHVQHGTIESILRYIKVKPGEF
                     YFIPAGTVHTISSGILAYETMQSSDITYRLYDFNRQDNQYNDRPLNIEKALDVIQYNA
                     PLPNILPESEIIENHKCTHIVSNDFFTLVKWEISGTLNYMKPREFCLVTVLEGEGQMI
                     VDGEIFKLTTGTNFILTSEDLDSVFEGDFTLMISYV"
     gene            31734..31964
                     /locus_tag="O2701_000439"
     CDS             31734..31964
                     /locus_tag="O2701_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292350.1"
                     /translation="MKQFLYIALVCGVIAGLGAFLHIPQYPSMTIPRIVAILGIISAM
                     LTFKDKQISASLKFSALLINVLPLCGTFVASN"
     gene            32636..34009
                     /locus_tag="O2701_000440"
     CDS             32636..34009
                     /locus_tag="O2701_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HCZ4292351.1"
                     /translation="MTAETNYFWLNCGYNRWNHNEPLVGQTALFESGAHFNPSQGFRA
                     FKKAKVGDQVIFYQVQTDTGLLGCGEIISVETGAQNKIRVQFRFNEQLKPLTADYLKR
                     SEALEFRISNMKETLFNQITAEEFDLISGLGKGEIKIPRYFFLAETEEFEPGNQYTIY
                     THTYNGIKRNGYHFYTQLEEGDNIIFYNRTKNQSVVGIGEVSKHIHEKPPIPGRTNST
                     VIEVSYEKDITPITLSTLNKHPKLKNLYFLQENAKQAIASMSQAQYDAIIEMSDNNGL
                     KSPFEMVQKPDMLESEKEEALKPFILLVVDRKEEGLKAANDLLQKANANPVITTGHPD
                     FSEDMLYGKYLPNETGALYYREGFITQLMPKKDKSYLVIDNFNRIDTDIFQTYINVLE
                     GYEVTLPRYNKDGNMIKWSRQKDSFYYFNPNWHIVGITYDSLEEIKEKYSEQFLKYTR
                     IVKVKHD"
     gene            34467..34880
                     /locus_tag="O2701_000441"
     CDS             34467..34880
                     /locus_tag="O2701_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HCZ4292352.1"
                     /translation="MPIVYYDGNCIYCYNYVIWLIQHDLPRNYQFATLKGEVGQQFFK
                     QHPEAANKNSVILQKGDRIYFESQAIIKLITALPNRTKLLGVGLWLVPKPVRNFGYRM
                     FANNRNRMWKTTWHQPNDYEKSFFLDDNAKVKLTD"
     gene            35080..35523
                     /locus_tag="O2701_000442"
     CDS             35080..35523
                     /locus_tag="O2701_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HCZ4292353.1"
                     /translation="MSNQQDVVKELNQQVANWTVAYTKLHNFHWYVKGPNFFSLHVKF
                     EELYNEASQYVDELAERILAVGGNPVGTLTECLEQSIVKEAAKGYSAEQMVEELSQDF
                     TNISKQLENAIEIAGNAGDDVSEDMFIGMQTSVDKHNWMFKSYLS"
     gene            complement(35644..36354)
                     /gene="deoD"
                     /locus_tag="O2701_000443"
     CDS             complement(35644..36354)
                     /gene="deoD"
                     /locus_tag="O2701_000443"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCZ4292354.1"
                     /translation="MTKGTPHIQPNGVKIAKTVLMPGDPLRAKYIADNFLENVEQFND
                     VRNMFGYTGTYKGKEVSVMGSGMGIPSIGIYSYELYNFFDVDTIIRIGSCGALQENVN
                     LYDVIIAQAASTNSNYVDQYNIPGHFAPIADFELVTKAKNVADQIGATTHVGNVLSSD
                     TFYNADPTFNDAWKKMGILGIEMESAGLYLNAIHAGKKALGIFTVSDHILRDEATTPE
                     ERQNSFTQMMEIALEIAE"
     gene            36669..>37006
                     /gene="deoC"
                     /locus_tag="O2701_000444"
     CDS             36669..>37006
                     /gene="deoC"
                     /locus_tag="O2701_000444"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCZ4292355.1"
                     /translation="MNSAKLIDHTLLKPESTRTQIDQIIDEAKAYHFKSVCVNPTHVK
                     YAAERLADSEVLVCTVIGFPLGASTTATKAFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQDIEAVV"
BASE COUNT        13678 a         5534 c         6515 g        11279 t
ORIGIN      
        1 attaattcct tctatagcac cattattaaa tgctgggtaa taaattgtat ttcttaacat
       61 cctttgatgt tttctataat atttaaccgc tttccataca cccttactca cagacttttt
      121 actaactgaa tttaaacgat taataaattt aggccaatta cataacctta ggtcttttcg
      181 taatccttgg acaagttcgt aggagtgtcg tagtatatcg tcttttgaaa gcatgaattc
      241 tacaatgtca gatgagcgtt tataagcctt aaaagattta ttccatctgt atttactaaa
      301 tatggtttta ctagtatcca tcaataggac tttccagtta ttcttaaaaa ttgtataatc
      361 aggtcctttt ttattacggt attcattcat aacttgtaca cgatacttat taagttctct
      421 atttaaatgt tgaacgatat ggaatctgtc aaaaataata gctgcattag gaaataggtc
      481 gcgaaataat cggacatagg gttcatacat gtcaatagta accgtcttaa cttgttgtcg
      541 attttttaaa tcgaatcgct cgaaataggc acgcaagaat cttgtagttc tattttctaa
      601 aatatctata acatcatgag tatcattatc tataaaaatg aaactcattg atccagttac
      661 atttttaacg cttttaaatt catccatagc gatgtgttct ggcaaacaat taaagggttt
      721 aattcgtaat gaattagccg ctttagttct aatacgactt acagtagatg gagagataca
      781 gttatcttct gcagtatcaa tctctgtttt aactttagta gcttcttcta taactttttg
      841 agtaacacat cttgagataa aacacgactc atcaacaatt gacgttttag cagtaaaagt
      901 gccattgcat tctaagcatt taaaacgttg tttagctaag ttgaggtatg tgttataccc
      961 ttgagtttta agtaaagtaa tacgagaaac acgcttacca tgtttatgaa tttgtccttc
     1021 atttttaact ccacaacaat aacaggccga aggcttatac gttagtacag cattaaccac
     1081 tgttgctttt tttcctttga taatgacgtc aatttcttgg tttatatatt ttatattttc
     1141 atcttttatt cttagtaatt ctaaggtatc attacacata ggcgcacttg tctcctttag
     1201 tttgggtttg gtcactttaa attatagagg caattgcgct ttttgtgtat taaaaaaagc
     1261 aggaagtttt accttcccac cataaaagat gaagaacctt tttttatgaa gtaataagat
     1321 aaacagatta tatgtgtagc tattgcttga taatggtaag cgcatacatt attctttaat
     1381 tacatagagc aaagggggac gcttatgaca aagacaaaag caattgatat tataggtgca
     1441 ccatcaacat ttggacaaag aaaattaggt gttgatttag gaccaacagc aattagatat
     1501 gctggattaa tttcaagatt aaagcaatta gaccttgatg tatatgacaa gggggatatt
     1561 aaggtacctg ctgtgaacat tgaaaaattt catagtgaac aaaaaggatt aagaaattat
     1621 gatgaaatta tagatgttaa tcaaaaatta aataaagagg tttcagcatc aattgaaaat
     1681 aacagatttc ctctagttct tggtggagat cattctattg cggtaggttc agtatcagca
     1741 ataagtaaac attataataa tttaggtgtt atttggtatg atgcacatgg tgatttaaat
     1801 atacctgaag agtcaccaag tggaaatatt catggtatgc ctctaaggat tttgacaggc
     1861 gaaggtccca aagaactttt agaattaaat agtaatgtaa tcaagccaga aaacatcgta
     1921 ctaattggta tgagagattt agataaaggt gaaagacaat ttatcaaaga tcataatatt
     1981 aaaacattta ctatgtcaga tattgataaa ttggggataa aggaagtaat tgaaaataca
     2041 atagaatatt tgaagtcacg caatgttgat ggcgttcatt tatctttaga tgttgatgct
     2101 ttagatccgc ttgaaacgcc aggcactggt actagagttt tgggtggtct tagttataga
     2161 gaaagccatt ttgcattgga attactgcat caatcacatt taatttcctc aatggattta
     2221 gttgaagtaa atccattgat tgacagtaat aatcatactg ctgaacaagc ggtttcatta
     2281 gttggaacat tttttggtga aactttatta taaataaatg atttgtagtg tataaagtat
     2341 attttgcttt ttgcactact ttttttaatt cactaaaatg attaagagta gttataatct
     2401 ttaaaataaa ttttttctat ttaaatatat gttcgtatga cagtgatgta aatgattggt
     2461 ataatgggta ttatggaaaa atattacccg gaggagatgt tatggatttt tccaactttt
     2521 ttcaaaacct cagtacgtta aaaattgtaa cgagtatcct tgatttactg atagtttggt
     2581 atgtacttta tcttctcatc acggtcttta agggaactaa agcgatacaa ttacttaaag
     2641 ggatattagt aattgttatt ggtcagcaga taagtatgat attgaacttg actgcaacat
     2701 ctaaattatt cgatatcgtt attcaatggg gggtattagc tttaatagta atattccaac
     2761 cagaaattag acgtgcgtta gaacaacttg gtagaggtag ctttttaaaa cgctatactt
     2821 ctaatacgta tagtaaagat gaagagaaat tgattcaatc ggtttcaaag gctgtgcaat
     2881 atatggctaa aagacgtata ggtgcattaa ttgtctttga aaaagaaaca ggtcttcaag
     2941 attatattga aacaggtatt gcaatggatt caaatatttc gcaagaactt ttaattaatg
     3001 tctttatacc taacacacct ttacatgatg gtgcaatgat tattcaaggc acgaagattg
     3061 cagcagcagc aagttatttg ccattgtctg atagtcctaa gatatctaaa agtttgggta
     3121 caagacatag agctgcggtt ggtatttcag aagtatctga tgcatttacc gttattgtat
     3181 ctgaagaaac tggtgatatt tcggtaacat ttgatggaaa attacgacga gacatttcaa
     3241 acgaaatttt tgaagagttg cttgctgaac attggtttgg cacacgcttt caaaagaaag
     3301 gtgtgaaata atatgctaga aagtaaatgg ggcttgagat ttattgcctt tcttttggca
     3361 ttgtttttct ttttatctgt taacaatgtt tttggaaata tctttaacac tggtaatctt
     3421 ggtcaaaagt ctagtaaaac gattcaagat gtacccgttg aaattcttta taacactaaa
     3481 gatttgcatt taacaaaagc gcctgaaaca gttaatgtga ctatttcagg accacaatca
     3541 aagataataa aaattgaaaa tccagaagat ttaagagtag tgattgattt atcaaatgct
     3601 aaagctggaa aatatcaaga gaagtatcaa gttaaagggt tagctgatga cattcattat
     3661 tctgtaaaac ctaaattagc aaatattacg cttgaaaaca aagtaactaa aaagatgaca
     3721 gttcaacctg atgtaagtca gagtgatatt gatccacttt ataaaattac aaagcaagaa
     3781 gtttcaccac aaacagttaa agtaacaggt ggagaagaac aattgaatga tatcgcttat
     3841 ttaaaagcca cttttaaaac taataaaaag attaatggtg acacaaaaga tgtcgcagaa
     3901 gtaacggctt ttgataaaaa actgaataaa ttaaatgtat cgattcaacc taatgaagtg
     3961 aatttacaag ttaaagtaga gccttttagc aaaaaggtta aagtaaatgt taaacagaaa
     4021 ggtagtttag cagatgataa agagttaagt tcgattgatt tagaagataa agaaattgaa
     4081 atcttcggta gtcgagatga cttacaaaat ataagcgaag ttgatgcaga agtagattta
     4141 gatggtattt cagaatcaac tgaaaagact gtaaaaatca atttaccaga acatgtcact
     4201 aaagcacaac caagtgaaac gaaggcttat ataaatgtaa aataaatagc taaattaaag
     4261 gagagtaaac aatgggaaaa tattttggta cagacggagt aagaggtgtc gcaaaccaag
     4321 aactaacacc tgaattggca tttaaattag gaagatacgg tggctatgtt ctagcacata
     4381 ataaaggtga aaaacaccca cgtgtacttg taggtcgcga tactagagtt tcaggtgaaa
     4441 tgttagaatc agcattaata gctggtttga tttcaattgg tgcagaagtg atgcgattag
     4501 gtattatttc aacaccaggt gttgcatatt taacacgcga tatgggtgca gagttaggtg
     4561 taatgatttc agcctctcat aatccagttg cagataatgg tattaaattc tttggatcag
     4621 atggttttaa actatcagat gaacaagaaa atgaaattga agcattattg gatcaagaaa
     4681 acccagaatt accaagacca gttggcaatg atattgtaca ttattcagat tactttgaag
     4741 gggcacaaaa atatttgagc tatttaaaat caacagtaga tgttaacttt gaaggtttga
     4801 aaattgcttt agatggtgca aatggttcaa catcatcact agcgccattc ttatttggtg
     4861 acttagaagc agatactgaa acaattggat gtagtcctga tggatataat atcaatgaga
     4921 aatgtggctc tacacatcct gaaaaattag ctgaaaaagt agttgaaact gaaagtgatt
     4981 ttgggttagc atttgacggc gatggagaca gaatcatagc agtagatgag aatggtcaaa
     5041 tcgttgacgg tgaccaaatt atgtttatta ttggtcaaga aatgcataaa aatcaagaat
     5101 tgaataatga catgattgtt tctactgtta tgagtaattt aggtttttac aaagcgcttg
     5161 aacaagaagg aattaaatct aataaaacta aagttggcga cagatatgta gtagaagaaa
     5221 tgcgtcgcgg taattataac ttaggtggag aacaatctgg acatatcgtt atgatggatt
     5281 acaatacaac tggtgatggt ttattaactg gtattcaatt agcttctgta ataaaaatga
     5341 ctggtaaatc actaagtgaa ttagctggac aaatgaaaaa atatccacaa tcattaatta
     5401 acgtacgcgt aacagataaa tatcgtgttg aagaaaatgt tgacgttaaa gaagttatga
     5461 ctaaagtaga agtagaaatg aatggagaag gtcgaatttt agtaagacct tctggaacag
     5521 aaccattagt tcgtgtcatg gttgaagcag caactgatga agatgctgaa agatttgcac
     5581 aacaaatagc tgatgtggtt caagataaaa tgggattaga taaataaata ctgtattaca
     5641 aatgagccga tgcgtatgca tcggtttttt gtgtttgtag aaataattta tagtacaaac
     5701 gtaaaatgat ataaaaaaaa taaaaacaaa gtaatcaata tgtaatataa aatacactgg
     5761 tactcaatat ataatgatga taaaattaat tttaattaga tagagttgct ttgtgttttt
     5821 aacgcagatg ctactactta tcttaacagt tgattaagtg aaatcattta acagcgagaa
     5881 taatcaacca ggaggatgac ttaatgaatt tattcagaca acaaaaattt agtatcagaa
     5941 aatttaatgt cggtattttt tcagctttaa ttgccactgt tacttttata tctactaacc
     6001 cgacaacagc gtctgcagca gagcaaaatc agcctgcaca aaatcaacca gcacaaccag
     6061 ctgatgccaa tacacagcct aacgcaaatg ctggtgctca agctaatcct acagcacagc
     6121 cagctgcacc tgccaaccaa ggacaaccag cagtacaacc agcaaaccaa ggtggacagg
     6181 ctaatccagc aggaggagca gcacaaccaa atacacaacc agctggacaa ggtaatcaag
     6241 ctgatccgaa taacgctgca caagcacaac ctggaaatca agcaacaccg gcaaaccaag
     6301 caggtcaagg aaataaccaa gcaacaccta ataataatgc aacaccggca aatcaaacac
     6361 agccagcgaa tgctccagca gcagcgcaac cagcagcacc tgtagcagca aacgcacaaa
     6421 ctcaagatcc aaatgctagc aatactggtg aaggcagtat taatacgaca ttaacatttg
     6481 atgatcctgc catatcaaca gatgagaata gacaagatcc aactgtaact gttacagata
     6541 aagtaaatgg ttattcatta attaacaacg gtaagattgg tttcgttaac tcagaattaa
     6601 gacgaagcga tatgtttgat aagaataacc ctcaaaacta tcaagctaaa ggaaacgtgg
     6661 ctgcattagg tcgtgtgaat gcaaatgatt ctacagatca tggtaacttt aacggtattt
     6721 caaaaactgt aaatgtaaaa ccagattcag aattaattat taactttact actatgcaaa
     6781 cgaatagtaa gcaaggtgca acaaatttag ttattaaaga tgctaagaaa aatactgaat
     6841 tagcaactgt aaatgttgct aagactggta ctgcacattt atttaaagta ccaactgatg
     6901 ctgatcgttt agatttacaa tttattcctg acaatacagc agttgctgat gcttcaagaa
     6961 ttacaacaaa taaagatggt tataaatact attcattcat tgataatgta ggtctattct
     7021 caggatcaca tttatatgtc aaaaatagag atttagcacc gaaagcaact aacaataaag
     7081 aatatactat taatactgaa atcggtaaca atggtaattt tggtgcttca ttaaaagcag
     7141 atcaatttaa atatgaagta acattaccac aaggtgtaac ttacgttaat aattcattaa
     7201 ctacaacatt ccctaatggt aatgaagaca gtacagtatt gaaaaatatg actgttaatt
     7261 atgatcaaaa tgcaaataaa gttacattta caagccaagg tgtgacaacg gcacgtggta
     7321 cacacactaa agaagtttta ttcccagata aatctttaaa attatcatat aaagttaatg
     7381 ttgcgaatat cgatacacct aaaaatattg attttaatga aaaattaaca tatcgtactg
     7441 cttcagatgt tgtaattaat aatgcgcaac cagaagttac actaactgca gatccatttt
     7501 cagtagcggt tgaaatgaac aaagatgcgt tgcaacaaca agtaaactca caagttgata
     7561 atagtcatta cacaacagca tcaattgcag aatacaataa acttaaacaa caagcagata
     7621 ctattttaaa tgaagatgcg aatcatgtta aaactgcaaa tcgtgcatct caagcggata
     7681 ttgatggttt agtaactaaa ttacaagctg cattaattga taatcaagca gcaattgctg
     7741 aattagatac taaagctcaa gaaaaggtta cagcagcaca acaaagtaaa aaagttacgc
     7801 aagatgaagt tgcagcactt gtaactaaaa ttaacaatga taaaaataat gcaatcgctg
     7861 aaattaataa acaaactaca gcacaaggtg tcacaactga aaaagataat ggtatcgcag
     7921 tgttagaaca agatgtgatt acaccaacag ttaaacctca agcgaaacaa gatattatcc
     7981 aagcagttac aactcgtaaa caacaaatta aaaagtcaaa tgcatcatta caagatgaaa
     8041 aagatgtagc aaatgataaa attggtaaaa ttgaaacaaa ggcaattaaa gatattgatg
     8101 cagcaacaac aaatgcacaa gtagaagcca ttaaaacaaa agcaatcaat gatattaatc
     8161 aaactacacc tgctacaaca gctaaagcag cagctcttga agaatttgac gaagttgttc
     8221 aagcacaaat tgatcaagca cctttaaatc ctgatacaac aaatgaagaa gtagcggaag
     8281 ctattgaacg tattaatgca gctaaagttt ctggtgttaa agcaattgaa gcgacaacga
     8341 ctgcacaaga tttagaaaga gttaaaaacg aagaaatctc aaaaattgaa aatattactg
     8401 actctacgca aacaaaaatg gatgcctata atgaagttaa acaagctgca acagctagaa
     8461 aagctcaaaa tgctacagtt tcaaatgcaa caaatgaaga agtagcagaa gctgatgcag
     8521 cagtagatgc agctcaaaag caaggtttac atgacatcca agttgttaaa tcaaaacagg
     8581 aagttgctga tacaaaatca aaagtattag ataaaatcaa tgcaattcaa acacaagcaa
     8641 aagttaaacc tgcagctgat acggaagtag aaaacgcata taatacacgt aaacaagaaa
     8701 ttcaaaatag caatgcttca actacagaag aaaaacaagc tgcatataca gaattagata
     8761 ctaaaaagca agaagcaaga acaaatcttg atgctgcaaa tacaaacagt gatgtaacaa
     8821 cagctaaaga caatagtatt gctgcaatta atcaagttca agctgccaca actaagaaat
     8881 cggatgcaaa agcggaaatc gctcaaaaag caagtgaacg taaaacagca attgaagcaa
     8941 tgaatgattc gactactgaa gaacaacaag cagcgaaaga caaagtggat caagcagtag
     9001 ttactgcaaa cgctgatata gataatgctg cagcaaacaa tgatgtggat aatgcaaaaa
     9061 ctacaaatga agctacaatc gcagccatta cacctgatgc aaatgttaaa ccagcagcaa
     9121 aacaagcaat tgcagataaa gtacaagctc aagaaacagc aattgatgga aataacggct
     9181 caacaactga agaaaaagca gctgctaaat aacaagttca aactgaaaaa acaacagctg
     9241 atgccgcaat agatgcagca catacaaatg cggaagttga agcggctaaa aaagcagcaa
     9301 ttgctaaaat tgaagcgatt cagccagcaa caacaactaa agataatgcg aaagaagcaa
     9361 ttgctacgaa agcgaatgaa cgtaaaacag caatcgctca aacgcaagac attactgctg
     9421 aagaaattgc agcggctaat gcggacgtag ataatgctgt gacacaagca aatagcaaca
     9481 ttgaagctgc taatagtcaa aatgatgtag accaagcgaa aacgacaggt gaaaatagta
     9541 ttgatcaagt aacaccaaca gttaataaaa aagcaactgc acgtaatgaa atcacagcaa
     9601 ttttaaataa caaattgcaa gagattcaag ctacgccaga tgcaacagat gaagaaaaac
     9661 aagcagctga tgctgaagca aatactgaaa atggtaaagc aaatcaagcc atttcagcag
     9721 caactactaa cgcacaagtt gatgaagcta aagcaaatgc agaagcagcg attaatgcgg
     9781 taacaccaaa agttgtgaag aaacaagcgg ctaaagatga aattgatcaa ttacaagcaa
     9841 cgcaaacaaa tgttatcaat aatgatcaga acgctacaac agaagaaaaa gaagcagcta
     9901 ttcaacaatt agcaacagca gttacagacg cgaaaaataa tattacagct gcaactgatg
     9961 ataatggtgt agatcaggcg aaagacgctg gaaagaattc aattcaaagc acgcaaccag
    10021 caacagcggt taaatcaaat gctaaaaatg atgttgatca agctgtgaca actcaaaatc
    10081 aagcaattga taatacaact ggtgctacaa ctgaagagaa aaatgcagca aaagatttag
    10141 ttttaaaagc taaagaaaaa gcgtatcaag atatcttaaa tgcacaaaca actaatgatg
    10201 ttacgcaaat taaagatcaa gcagttgctg atattcaagg tattactgca gatacaacaa
    10261 ttaaagatgt tgcgaaagat gaattagcaa caaaagcaaa cgaacaaaaa gcgcttattg
    10321 cacaaactgc agatgcgact actgaagaaa aagaacaagc aaatcaacaa gtagacgcac
    10381 aattaacaca aggtaatcaa aatattgaaa atgcacagtc aatcgatgat gtaaacactg
    10441 caaaagataa tgcaattcaa gcaattgacc caattcaagc atcaacagat gttaaaacga
    10501 atgcaagagc ggaattgcta actgaaatgc aaaataaaat aactgaaata cttaataata
    10561 atgagactac taatgaagaa aaaggtaacg atattggacc agttagagca gcatatgaag
    10621 aaggtttaaa taatattaat gcagcaacta ctacaggtga tgtaactact gctaaagata
    10681 cagcagtaca aaaagttcaa caacttcatg caaatcctgt taagaaacca gcaggtaaaa
    10741 aagaattaga tcaagctgca gctgataaga aaacacaaat agaacaaaca ccaaatgcat
    10801 cacaacaaga aattaatgat gcaaaacaag aagttgatac tgaattaaat caagcgaaaa
    10861 caaatgtcga tcaatcatca acaaatgaat atgttgataa tgcagttaaa gaaggaaaag
    10921 ctaaaattaa tgcagttaaa acatttagtg agtacaaaaa agatgcttta gctaaaattg
    10981 aagatgcata taatgctaaa gtaaacgaag cggataactc taacgcatcg acttcaagtg
    11041 aaattgctga agcgaaacaa aaacttgctg aattaaaaca aactgcggat caaaatgtta
    11101 atcaagctac ttctaaagat gacattgaag ttcaaattca taatgactta gataatatta
    11161 acgattacac aattccaaca ggtaaaaaag aatcagctac aacagattta tatgcttatg
    11221 cagatcagaa gaaaaataat atttcagctg acactaatgc aacacaagat gaaaagcaac
    11281 aagcaattaa gcaagttgac caaaatgttc aaactgcatt agaaagcatt aataatggtg
    11341 tggataatgg tgacgttgat gatgcattaa cacaaggtaa agcagcaatt gatgctattc
    11401 aagtagatgc tactgttaaa cctaaagcga accaagctat tgaagttaaa gcagaagata
    11461 cgaaagaatc tattgatcaa agtgaccagt taactgctga agaaaaaact gaagcattag
    11521 caatgattaa acaaattaca gatcaagcta aacaaggtat tactgatgca acaacaactg
    11581 ctgaagttga aaaagcgaaa gctcaaggac ttgaagcatt tgataacatt caaatcgact
    11641 caacagaaaa acaaaaagct atcgaagaat tagaaactgc actagaccag attgaagcag
    11701 gtgtaaatgt caacgctgat gctacaactg aagaaaaaga agcgtttacg aatgctttag
    11761 aagacatttt atcaaaagca actgaagata tttctgatca aactacaaat gcagaaatcg
    11821 ctactgtcaa aaatagtgcg cttgaacaac ttaaagcaca acgtattaat cctgaagtta
    11881 agaaaaatgc tttggaagca atcagagaag tggttaacaa gcaaatagaa ataattaaaa
    11941 atgcagatgc agatgcatcg gcgaaagaaa ttgcacgtac ggatttaggt agatattttg
    12001 acagatttgc tgataaatta gataaaacac aaacaaatgc agaagttgct gaattacaaa
    12061 atgtaacgat acctgcaatt gaagcgattg tgcctcagaa tgatccagat gctaatgata
    12121 ctaataatgg tatagataat aatgatgcaa cagcaaattc aaatgctaat gcaacaccag
    12181 aaaatactgg acaacctaac gtatctgaga cgacagctaa tggcaaagca gacgcttctc
    12241 cgacaacacc aaataatagt gatgcggcaa caggtgaaac aacagcaaca tcagcaactg
    12301 atgatgcaaa tgataaaccg caagctaata acaattctag tgtagatgca tcaacaaaca
    12361 gtccgacaat ggataacgat gtaactagta aaccagaagt agaatcaact aataacggca
    12421 caactgataa acctgtaaca gaaactgata atgcaacgcc agcagaaagc acaacaaata
    12481 acaatagtac aactacagca acaaacgaga atgcaccaac aggatcaaca gcaacggctc
    12541 caacaactgc gagcacagaa gcagcatcat ctgctgatag caaagataat gcatctgtaa
    12601 atgattcaaa acaaaatgct gaagtaaata atagtgctga atctcaatca actaatgaca
    12661 aggttgcaca accaaaatct gaaaataaag ctaaggcaga aaaagatggt agtgattcaa
    12721 caaatcaaag tatggttgaa tcaacaactg aaacattgcc ttcagcagac ataacagaac
    12781 caaatgtacc ttctaataca tcaaaagata aagaagaaag tacgacaaat caaactgatg
    12841 caggacaact taagtctgaa actaacgttg catctaacga agcagataaa tcgccaagca
    12901 aagctgatac tgaagtttcg aataaaccat caacatctgc atcttctgaa gcaaaagaaa
    12961 aaatgacttc aactaatgtt agccaaaaag atgatacggc aacagcagat actaatgata
    13021 cgcaaaaatc agttggttca gctgcaaaca ataaagctac gcaaaacgac ggtgccaatg
    13081 catctccagc tacagtttca aatggaagca atagtgctaa tcaagatatg cttaatgtaa
    13141 ctaacactga cgaccaccaa gctaagacaa aatcagctca acaaggaaaa gttaataaag
    13201 ctaaacaaca agctaaaact ttaccagata ctggtatgtc acataatgac gatttaccat
    13261 atgctgaatt agctctaggt gcaggtatgg cattcttaat tagaagattc actaagaaag
    13321 accaacaaac tgaagaataa aatttattca agaatgtgaa catctagtta tttcataggg
    13381 aaatattcct tactaagtaa ccaagtctaa cagttaatat gaatttgttt tagtaatgac
    13441 caatagccat tgaataggtg gctgtggctc gaaaataaaa caaattatta acgcaaaaat
    13501 actcctttca tacataacac aagttaagtg tctatgttga agggagtatt ttttatgttc
    13561 agaaatttaa tattgttaat tcaagggaac tactacatta ttactgattg ttaacgaata
    13621 acgttggaaa cttcttgttt caaacaaagc tcaataaatt atgaaagtga attgtacgct
    13681 tcaattattt catcggctaa gccatcgtca actttagcat attcacttaa aaacgctttt
    13741 aaaccgtgtt ctttaatgta attatttttc tcaacagttt cttggtctgc tgtatcatca
    13801 tacttcaata acaatgcagc tgtatttaat aaaccagtgc gttctaaatc tttattataa
    13861 agatatgtta atggttttat aattctatct ttagggccaa ttttacgtaa tgtacctcgt
    13921 ccgacacgtg ttacttcatc agataaataa gaattgttga aacgatctat tattttttca
    13981 acataagcag cttgttccgc ttcagtaaaa tcaaattcat tagtaatata ttgactagtt
    14041 tcagctaaaa cacggcgtaa gccagcttca attgaactat cttcaactgc atctaaaact
    14101 gtagctttac ctgcaaattt accagcatac gctaaatatg catgtcctgt attcacagtt
    14161 aataatttac gctcaatata tggtgttaaa tcatcaacat atttaatatg gtttagttct
    14221 ggaccatacc atgcatcttt ttcaacaacc cattcgtaaa atggctcaac cataacgtct
    14281 aatatatttt cattcttttg taatggtaca attctatcaa ctgctgagtt agcaaaatga
    14341 atattgttac caagagggcc agtaatatca agtactgctt ttttcaatgt atcagttgcc
    14401 ataatagcat tctcacaagc aacaatatta acatggtttg ttttttcttt taagaaaggc
    14461 gcaaaagatt tagcaataat aggtagtatg ttaacaccaa cagctgttgt aataatatca
    14521 gcttctagaa ttgcttgttt caacgcttca gaaggttgca ttgaattaat tgcatcaaca
    14581 ttattcacgc gcgtcgttgt tttagactca tcagctaaaa taacatcgta ttgatgatca
    14641 tgagctaaag cattaatgat ttcttcattg acgtctgcaa atgttacttt aacattgttg
    14701 tctgcaagaa tataaccaat gaaaccacga cctatgttac cagcaccaaa gtgaactgct
    14761 ttcattatgc atctgcctcc tcgaatactt gtttaatttc ttctgcagat tttgcttgaa
    14821 cgatacgatc cacattttct tcttcgctaa atgtaattgc aattttagat aacaagtcta
    14881 aatgttcgcc atctttacca gcaattccca cgacaacttt aactacttcg ccatcccagt
    14941 caacgccttc agggatttgt aataatgtta aacctgagtg aatcacattt gttttagctt
    15001 catctgtgcc atgaggaatt gctaagccat ttcccataaa tgttgatacg acttgctcac
    15061 gatctttcat tgcttgaata taagcatctg ttacagcacc actatcaact aaggctttac
    15121 ctgctttttc aattgcttca ttttggctgt taacatttac atttaaaaag atattgtcat
    15181 tactaaataa ttcgctcatt ttgtacactc ctaaataaat tgttttttaa ttcgttctaa
    15241 aaatttgttt cttaataatg tttcaagttc ctgtggattt tccataaaag tatcaataga
    15301 ttctaatttc aaacttaaaa attcggacaa gtcacttacc agtgatgcca taaaatcatt
    15361 gtcagaaata aacatggaaa tcatatattt aattgtttga ttgtcatttt gaatactagg
    15421 caatgttaac ggttcttcta aaactgtgat tagaatcata gggtgtttaa tcatattgtc
    15481 tcttaggtgt ggtattgcaa caggatatgg acttaatatc catcctggat tgtcaatcaa
    15541 tcgttgctct agtagttcag cgaatgacgt tggatcgttt attacatcgc attgatatag
    15601 ataatctgcg atatatgttt gccactgttt aacttcggta ctaactattt tcactgaatc
    15661 aagcaaagtt aaacccgaac gcatatgatg caataattgt tctgattttt cgtcacttaa
    15721 atgtttttcg aaaactgaag ttaagtatga agtttgttca ttttcaaaag atgtgttgcc
    15781 atcaacatct ttcgtttcaa catgaacatc atccttatca atcatgtttg atgatttatc
    15841 atgcgtctct tggaacgtag actttgtatt taaaaactgt gcgacataac tgatatcact
    15901 atctggtaat aatgggttta ccgttaaata gggggagtcg atgtctaaat tcacagtaga
    15961 aataatgcca tcatattgac ttaaatctag tgacttcaaa tcgctgactg atgcttgtgt
    16021 aatacgctca atctcactaa aaacttgatc tagacgagtt gataatagac gactagttcc
    16081 cataccactg ctgcaaacga ctaatatgtt taaaaatcga ttaccttggt ttttaatcga
    16141 gccaccaaag tgtaaaacta taaaagcaat ttcactctct ggaaaaatca aatcaggcca
    16201 acaatcattt aaagctaatc ttacattttc aaatagtctt ggatatttga acttaatcat
    16261 gtctgttaac ggattatagg tctcgatatt agcttgcaaa cgattgattg caggattaag
    16321 gtgtagcgtc agtccatcta ctaaagtatc caaatctgca aatgtcatac cggaaatcat
    16381 ttctactttg ttaacaaacg ctttaatttt gttttcttca caacgattat ttaatgatgt
    16441 atcattaaga ttttttcgtt tagctccacg taaatggata gtaatgaaag caatttctgc
    16501 ctgattaaac gtgacgtcat atatttgacc aagtttatca gcaagttcgc ttgctacttt
    16561 gtgttcaaat gtgttttgta cagaatcata aatatcatcg tttaatgcga catactctcc
    16621 attttttata cgtgaaatgg agagcacgat atggacagtt aaagttaaat aacttgattc
    16681 ggtaagttgg tagggtaagg cacttaggta gtccattaat agtctttcaa cattaaaaat
    16741 gcgctctaag tcaacaaagt ctttgtggat ttgatttaat gaatgaaaga caaaatgatt
    16801 ttcaattact gaataaacgc tagtactatt taagttattc accattaatt gacttaaaaa
    16861 ttcacgtttc tttgattcag tacctaccaa gtaaatgcct tcgccacgct ttcgagataa
    16921 agatagttgg tacttattta aatcaagctc taaatcatct aacatctttg ctaaagtctg
    16981 gacagaaacg ccaatttctt gcgctaaact atattgttta actggctcct tggcttgtat
    17041 caaagcgtat ataataatta ctttctgctc ttcaacagat aagtcaatgg tttgatgttg
    17101 tgcaatcgat tgcttcaaat cgtttaaatc agaatctgtg ccagcaatgc gtagcccttt
    17161 tttgtttgcg cgttctaaag ttaatgaaaa tgaagttaaa tatgcttcaa caccttttaa
    17221 ttcacggtga atagtacgag aggacaccgc aagttgctga gcaatgtcgt gtatagtgat
    17281 atattgaccg tggtacttaa tcaatagggc tatcatttct ttttcacgtg tactcaataa
    17341 catggcggta tcctccattt tttaatttat ttaattatta tgcttgatca tcttttttta
    17401 gattatttaa aagttcttca tatcttggtg aattaaggaa attatccact gaaatatgga
    17461 tggcatttgg tgtttgttta atagcacgat cagttagttt tttctgagtg ataactaatt
    17521 gagcatcttt tggcaattga ttaatcgcag tatttgtaac tgtaatatca ttaatgcccg
    17581 cctttttaaa tttattacgt aacatgcttg cacccattgc actagaaccc atacccgcat
    17641 cgcaggcaaa aataacatga tttatattat tgtaattgtg tgcatcaaca tcttcagtgt
    17701 tgtaattatc caatagcgct tcaggatcat catctgatga agatgtttca ctaacattac
    17761 cactagcatt ttcttctgta ttaacatttt tatcagaaga tactaactta gaagcaacgc
    17821 ttgatttttt ccctttagta ttttccattt gagctgtcgc agcttctaaa tcctgctttg
    17881 gttctctagt gaacttcata attaaagcag ctacaacgaa tgatacaagt gcagcaagga
    17941 agacaccgag taacatgtgc aagaattcac ctctaggtgc atttaaacag taaactataa
    18001 atgaacctgg tgacgcggga cttttaaatc caaatcctgt tgcttgataa gttgcaacac
    18061 cagtcattcc acctaaaata acagcgataa ataataaagg acgcattaat acatatggga
    18121 aataaatttc atgaatacca cctaagaagt ggataattcc agcaccatat gacgttgctt
    18181 ttgcagtgcc ttttccaaaa atcatataag caagtaagat acctaaacct ggtccagggt
    18241 tagattcaat tgtgtataaa attgattgac cagcttttgc agcttgatct gcaccaagcg
    18301 gtgtgaatac accatggtta atcgcattgt ttaaaaatac aatttttgca ggctctacta
    18361 aaatacttac aagtggaagt aggtgtgcat gtactaatgc ttcaactgcc actgataaaa
    18421 tatgcataat aaatttcata agtggtgcta aaattttaaa tcctgcaatc gtcatgataa
    18481 atcctaaaat accagcagaa aagttattaa ataacatttc aaaaccttgc ggcgttctag
    18541 gttgaatcaa ttggtcggtc ttcttcatta accaaccaac aagtggaccc ataatcattg
    18601 caccaagtaa cattggtgta tcaggtaatg caacgatgac ccccatagtt gctgttgctg
    18661 cgatgatacc accacgtaaa tcataaatta aacgaccacc actaaatgcg atcaataatg
    18721 ggattaaata agtaatcatt ggtcctgcta aagtagctaa atctttgtta ggtaaccatc
    18781 cattatctat aaaaatggcc gcgataaaac cccaagcgat gaaagcgcca atgtttggca
    18841 tgatcatact acttaagaat gatccaaatg cttgaacacg acgaccaatt ccttttttct
    18901 cttcagtttg tgacatgttt acacctcttt tttaatatat gtgactatat atagtgtata
    18961 gagaggttac ttgttactca atataaacaa aaatcaactt tgtcaaaata aatgtgacaa
    19021 aattaaataa agtgtcatca atgtgacagt atagatattt tgaaaaagta aaacaaaaaa
    19081 attgttttag gatttttaaa attttattgt gaaaatattt gcaaaacaaa acaacaccgt
    19141 tgtacaataa tgattaatgg aaagggggaa agttcggcag tacagttaaa gcgcctgtgc
    19201 aaataaatat ttgtatttga agattaaagg ttaatatatg agtggccttt atagagtgca
    19261 atatatgtat ttgtagacga ggaggatagt gatcgaatag atcggcggat gctatcccgg
    19321 atgtggctca ttcgttagct tattaagtaa aacattaggg tgacttaatg gacaaagtta
    19381 ataagatcgc cagaaattga atataaaaaa tattaatatg gaaagtacag tgtgagcaat
    19441 ttgtatagtt gtaaaaataa ctatgcttaa tttgttatgg atgaatgcga tgatagcatg
    19501 ttcctattta tattatgaaa gcagattgtc aatctaaatt atcggcaata aatcataatt
    19561 tacgcgtact attccaatat ggaggaaaat gtcgttatgt gtggaattgt tggttatatt
    19621 ggctatgata atgccaaaga attattatta aaaggtttag aaaaattaga atacagaggt
    19681 tatgactctg caggtatcgc agtagtaaat gatgataata caactgtatt taaagaaaaa
    19741 ggtcgtattg cagaattacg taaagttgct gatagtagcg attttgatgg acctgttgga
    19801 atcggtcaca cacgttgggc aacacacggt gtaccgaatc atgaaaactc tcatccacat
    19861 caatcatcaa atggccgttt tactctagtt cataacggtg ttattgaaaa ctatgaagag
    19921 ttaaaaggtg aatacttaca aggtgtatca ttcatttcag aaacagatac agaagttatc
    19981 gttcaattag ttgaatactt ttcaaatcaa ggactttcaa ctgaagaagc atttacaaaa
    20041 gttgtgtcat tattacatgg ttcatatgca ttaggtttat tagatgctga agacaaagac
    20101 acaatctatg ttgctaaaaa taaatcacca ttattattag gtgttggtga aggtttcaat
    20161 gttatcgcat cagacgcact tgcaatgtta caagtgacaa gcgaatataa agaaatccat
    20221 gaccatgaaa tcgttattgt taaaaaagat gaagttatta ttaaagatgc agatggaaac
    20281 gttgtagaac gtgattcata tattgctgaa attgatgcat cagatgctga aaaaggtgtt
    20341 tatgcacact acatgttaaa agaaattcat gaacaaccag cagtaatgcg tcgtattatt
    20401 caagaatatc aagatgcaga aggtaacttg aaaattgatc aagacatcat caatgatgtt
    20461 aaagaagcag accgcattta cgttattgca gcaggtacaa gctaccatgc aggtttagta
    20521 ggtaaagaat ttttagaaaa atgggctggc gtaccaactg aagtacacgt tgcatcagag
    20581 tttgtctaca acatgccatt attatctgaa aaaccattgt tcgtttatat ttctcaatca
    20641 ggtgaaactg cagatagccg cgccgtatta gttgaaacta ataaattagg tcataaatca
    20701 ttaacaatca ctaatgttgc aggttcaact ttatcacgtg aagcagacca cacattgtta
    20761 ttacacgcgg gtcctgaaat cgcagttgca tctacaaaag catatactgc acaaattgca
    20821 gtattatcaa tcttgtctca aatcgttgca aaagagcatg gtcgtgaagc agatattgat
    20881 ttattgagag aattagcaaa agtaacaaca gcaatagaag caattgttga cgatgcacca
    20941 attatggaac aaattgctac agatttctta gaaacaacac gcaatgcatt ctttatcgga
    21001 cgtactattg actataacgt aagtttagaa ggtgcgttaa aacttaaaga aatttcttac
    21061 attcaagcag aaggttttgc tggtggagaa cttaaacatg gtacaattgc cttaatcgaa
    21121 gaaggtacac cagttgtagg tttagcaaca caagagaaag ttaatttatc aattcgtggt
    21181 aacgttaaag aggtagtagc acgtggtgca catccatgta ttatttctat ggagggtctt
    21241 gaaaaagaag gcgacactta tgtcattcct catgtacatg aattgttaac gccattagta
    21301 tcagtggttg cattacaatt aatttcatac tatgcagcat tacacagaga tttagatgtt
    21361 gataaaccac gtaaccttgc taaatcagtt actgtggaat aattcacttt tttagaatca
    21421 atcatgtatt aaaattaaag tatatggcac ccttttagat taatcgacta gaagggtgct
    21481 tttttaggtc gacttagctt ttacttcatc ttaatttggc agaaatgcgt aaaaatgaag
    21541 tgttttattt atttaaatag tctgacaatt aagggtgtta tgttaatatg attttatgag
    21601 aagtatggag tagcaataaa ggggtgacct cgcatgttaa ttcaattaga tcaaattggg
    21661 cgaatgaagc aaggaaaaac aattttaaaa aagatttctt ggcaaattgc taaaggtgat
    21721 aaatggatat tatatgggtt gaatggtgct ggcaagacaa cacttctaaa tattttaaat
    21781 gcgtatgagc ctgcaacatc tggaactgtt aaccttttcg gtaaaatgcc aggcaaggta
    21841 gggtattctg cagagactgt acgacaacat ataggttttg tatctcatag tttactggaa
    21901 aagtttcaag agggtgaaag agtaatcgat gtggtgataa gcggtgcctt taaatcaatt
    21961 ggtgtttatc aagatattga tgatgagata cgtaatgaag cacatcaatt acttaaatta
    22021 gttggaatgt ttgctaaagc gcaacaatat attggttatt tatctaccgg tgaaaaacaa
    22081 cgagtgatga ttgcacgagc tttaatgggg caaccccagg ttttaatttt agatgagcca
    22141 gcagctggtt tagactttat tgcacgagaa tcgttgttaa gtatacttga ctcattgtca
    22201 gattcatatc caacgcttgc gatgatttat gtgacgcact ttattgaaga aataactgct
    22261 aacttttcca aaattttact gctaaaagat ggccaaagta ttcaacaagg cgctgtagaa
    22321 gacatattaa cttctgaaaa catgtcacga tttttccaga aaaatgtagc agttcaaaga
    22381 tggaataatc gattttctat ggcaatgtta gagtaaatat tttgcaaata ataagtaata
    22441 atgacaaaat ttaattaaga taaaatggac agtggagggc aatatggata acgtaaaagc
    22501 aatatttttg gacatggatg gaacaatttt acatgaaaat aatcaagcat caacgtatac
    22561 gaaagatgtc attaatcaat tgagagagaa aggatataaa gtatttttgg caacaggacg
    22621 ttcgcattct gaaatacatc aacttgtacc tcaagatttt gcggttaatg gcatcattag
    22681 ttcaaatgga acaattggag aagtagatgg agaaattatc ttcaagcatg gtttatcatt
    22741 ggctcaagtg caacaaatta ctaatttagc taagcgccaa caaatttatt atgaggtatt
    22801 tccttttgaa ggtaatagag tttctttaaa agaagatgaa acatggatgc gagatatgat
    22861 tcgtagtcaa gatcctatta atggcgtaag tcatagtgaa tggtcttcaa gacaagatgc
    22921 gcttgctggt aagatagatt gggtaactaa gtttcctgaa ggtgaatatt caaaaattta
    22981 tctattcagt tctaatttag aaaaaataac agcatttaga gatgaattaa agcaaaatca
    23041 tgtgcaacta cagattagtg tttcaaattc atcaagattt aatgcggaaa caatggctta
    23101 tcaaactgat aaaggtacag gcattaaaga aatgattgca cattttggta ttcatcaaga
    23161 agaaacgtta gttattggag atagcgacaa tgatagagca atgtttgaat ttggtcatta
    23221 tacagttgct atgaaaaatg cacgccctga aatccaagca ttaacttcag atgtaacggc
    23281 atacacgaat gaagaggatg gcgcagcaaa atatttagca gagcattttt tagctgaata
    23341 ataaaatagg tagttattta ttatttaatt tacaatagtt gatgagtaat gtacaaagag
    23401 cagtaaagtt attttctatt agaaaatgtc ttactgctct tttgtatgct tataaatatt
    23461 tgaatcatct atatttaatt ggacaaactc tatgagaata aatattgtta aaactaataa
    23521 gataggaaat tcattgattt tgaataatat ttcttgtttt aaggtttaac tattgaattg
    23581 tatacttctt tttttagtag caacagatcc gtctttaaaa aaattcacac ctattgactg
    23641 tttttgttct tgattatatt ctctatgaag tgttaatcca ctataaaatc aaccggttcg
    23701 tttacatatt tccccaatat aatttactct atagtatact tcaaatatat ctactttaaa
    23761 ttatcatctc catactattt attgaataca agaaaataaa cataaaaggt gctataaaag
    23821 aacaaagtga ataaatagtg ttagttttaa atattttagt gtttttttct ttgtaaatat
    23881 gacattcaag actagagtta taaaaaagac taaacaggat aatgtgggta agcaaacaat
    23941 gaatgctaat gtatataaaa taaatatcat ccttgattta gtgttgtaga gggcatcacg
    24001 taatttgtga tgaataataa taagtatttg gaactttatt gcattttatc tatttttatt
    24061 aattggaatc tttatacatt ctaatgattt aacattatta tgaaaatggc ttttagatgt
    24121 tgttttaaaa ataaacactt tgaaatataa cctcttgctg atagattaag aaaatatatt
    24181 cttgaatttt aaggggggat aattacaatc ttagttcttt taactggtac tattatcaat
    24241 gaatcataaa attcgaaaca tacgagtttt tagactatga gagacacatt catttgcatt
    24301 gagaatcatt tttttagtgt ataatctatg taaatcgaat tagaatatag agattttgtt
    24361 tgttcggatg tattttttat tcatacttaa taaatttgaa aagcaaaaat gaggagatga
    24421 aagctagtga gcgtaaatgc acgtgattta ttagtattac acactaatgt aaatagattg
    24481 gttggagaag agatatttgc taataagtgc cttgctaata atgatgttca aattatgaat
    24541 tcaataaaaa aattaattga agctgagtta ttaacaacga ctaatgattt tgaagttagt
    24601 atatataaaa agacaagacc tgaattacaa agtattttaa aaagttttgg tataaaaaca
    24661 acaggtaata aacctgactt aattaaacgt attgacgaca attttcatat tattaataac
    24721 ttagatttac catatgtata tataccaact aagaaaggag aagaaatttt aaagaaaaca
    24781 gagtacttaa cctcttttat acaaagttat ggtgaaattt ctcttgagcg tgcttattat
    24841 ttggttgaaa actatataga tgaaaattgc gatgataaag ttgcagaaat atacaagttt
    24901 gaatttcaaa gaaaatatga caatggcgag tttgatttta atcatggata taatttcgaa
    24961 ttgaatatgt tgatagatca ctataaaaga gatgtaaaag actacgataa tgccagaaag
    25021 tattcaaata tttatcttta ctttggtttg agagatttct taaaaaaatt aatgagtaat
    25081 tattcatatt acgatagtaa agggaatata gatttgaacg aaatacaaaa cgatctgaat
    25141 agatttatca actctagcgc ttctggtatg tacgagcgat taatatataa tgaaaattta
    25201 tccaataata ttatgtttga attatttaaa aaggacacac aagattatag tgatttggaa
    25261 gaacaattga ttgaaaagtt cataaactat gtagtgtcta atgtaaaaaa agaaagtagg
    25321 agtaatactc ttatagagtt atcaaaaatt ttagagaacg gatatacaat tgataaagaa
    25381 gaatttaaaa aagaagatga ttatctttct aagtacatat ttactgacat agattatttg
    25441 aaaaagttag aatcaaaaat aaacgttgct attgatattc gaagtggaga aattcatttg
    25501 gtattagatg atgatagcct tgatatatta atacaaaatc aaaaatacgg caatgagttt
    25561 tgagtcataa ctaaatataa tatgttgaag agaggtttta tttaaatggc taaaattggt
    25621 tatgatcgtg tatcaacgaa agattaatat cgatacactt aaagaatttg ggtgtgggtg
    25681 tgtatatagt gaaagaataa gtggtgtcaa acgtacagag cttgttaagt gtttagatta
    25741 tttacgagag agcgacacat tagttgtcta tcaacttgat cggttaggta gaacgacaaa
    25801 actattaatt gaattatcac aatgattcga tgataacgga attgacttac aaattagtaa
    25861 catgaacatt tcaacgaaag acacaatggg caaaatgttt tttacgatga tgagtgcatt
    25921 tttcaggatt agaagttaat ttactatgtg agtgtataaa atagacttag cagcaacaag
    25981 agcgagaggc cgaaaaagcg ggcgcccctc tttaccagag aataaaaaat gagaaattaa
    26041 atttttatat gatgaacaaa cgataacagg ggaagaaata gctagttaga caagggtatg
    26101 tcactcaact gtttatcgag ttgattaaga aatgaaaaaa cttatactat gaattactgt
    26161 ttaaaagtgt gcatgttata atatttattg agcaagttgg atagatggtg gctaatctct
    26221 taataaaggg gtgatgccta tggttatagt tgttactcct agaaaggact agcatgtctg
    26281 attttgaaat gcttatggtt gtattaacaa tcattggttt agtattgatt agtactcaag
    26341 accataaaaa ataaccttct attcgctttg accggcattt ttgaaggcta tttttaaata
    26401 aaatataagg tcaccgtctt tttaacgggc tcattagggt aacatgtttc cgagtgttgc
    26461 cctttttgtg tttcaagagt taatgatttt catcttttgc ttctacttac tacaaatata
    26521 ttttaacaca tttttcttat gaattgtagt tctgaacata atcagaatta ataaaaccaa
    26581 ctttccatac agcagaaaat acaattaaaa gtatagaatg taatcacgaa ttatataaaa
    26641 agtattggag acctcttcat atatagataa ttcacttagt tattttagaa agaagcccct
    26701 aacaactaaa gttgaaaaat agaggaacac agttggatgc tacgcatcaa ctgcataaga
    26761 gcccctaaca actaaagttt aaaagtagga gcgcctgcgc taagcgcatg cataagagcc
    26821 cctaacaact aaagttgtaa ggggctctaa aatttatttt ggttgattgt cttctggttt
    26881 atctgaagtc attgtttttg ttgtattatc atttaatgaa tcttgtgctt tttctgtttt
    26941 tgtttcaaca gatgttgtca ctttatcatt ttctactttt gtattttttg gttcagtgac
    27001 aactttttta ttcttgtcag ctgtttttac tttatcttta gtaatttttt gaccacgttt
    27061 taagtaatat tgaacgatac ccattaaaat gattgcatga ataacaacga ataaaataat
    27121 tgtcattact gtataaactc ttatgatatt ttctgcaaca ctttgagaga gtgattgcgt
    27181 attaaatgaa atcaagtaac caacaggtgt tttaaataaa ataataatta agtttaataa
    27241 tagtattcca atgaaaaatt tgaaaaatgt tttttgacca tttttcattg ctttaaatcc
    27301 attagctaag tgtgttttta ctttatcgtt agttgattca acgaaactag tcataaagtt
    27361 gaaaataggt attaacaata accaagtaat aattgcttta atcaataaaa caacaatgat
    27421 tatgatgctt tgagttgtaa tagcaatacc aatcaggtgt gtactatctg catatgatga
    27481 ttgtactgaa ttcataatca tttctgatac tggagaaaat aatttgtata gtaatgatgt
    27541 cagaagtgaa agaacgataa tcattgctat agatacaaga ccgatcagaa cgctctttaa
    27601 ataattacct tttttaaatg caataaataa atcggtaaat tttacttttt catgactcat
    27661 tgctcgtttc ataacatttg taattccgat aaaaatttga actaaaacaa acagtgaaac
    27721 tacagctgct ataagtaata cagcgataac ttttaaatat gcatctactg gtggtttttg
    27781 cccaaattgt gaataaattg caatagtttg tgcatactta gcaagctgga aattagcaag
    27841 taaatataca actgcaatta cagcaaatgc tataagtgca tatatcaatg ttattaataa
    27901 ttgtggttta gcatttttaa atgctgattt aaagtaagta aacaaagtgg tgcctccttt
    27961 tcttcatata aatacaataa caaaaaacac gttattttca tattaatatc taataaaaat
    28021 taattaaagt gactaaagtt agagaattat aatagtaaat tgtcaaaaat atgactaatg
    28081 taagaaataa tagttgaaaa tttgttaata aacattgcat catcaaagac caaacaagtc
    28141 gccataaaga cataaaaaac ctactgccat tgaaaagttt aatcgatgca atggcagtag
    28201 gttaaaatta ttaatgtaca tgcgcgtgat gatgagcatg atggttatgt gaatgactat
    28261 gtgttcctga acatataatc gattcatcat gtttgtgatt aggcgtttct aattgaatag
    28321 tcatatggtg aatatttaaa tgtaataaat catgctcaat gttttctaat aataattcac
    28381 attctttcat tgtcaatgta tggtctacaa caacatgaca actcaatgca ttcatatcat
    28441 ttgaaattgt ccaaacatgg caatcatgca cactttgtat tcgtgaatcc tttttaatag
    28501 tagttataac ttcatctata tcaacatcac ttggtgtgcc ttccattaaa atgttaattg
    28561 aagattttgt gataccccaa gcacttttta aaataataac ggaaactaaa atacttgcga
    28621 taggatcggc gattgtccat ccaaatgccc aaattaaaat agctgcagta atggcgccaa
    28681 ctgaacctaa taagtctccg ataacatgta gaaaagcacc acgcatattt aaattgtgtg
    28741 aagtgtcgcc gcctttaaac ataaagaatg caacaacgat attgacaatt aaaccgataa
    28801 tactaatgat taacatttct tttgattgaa cttcagaagg aacaaagaaa cgtttaatcg
    28861 cttcaaaaac aatcaaaata cttattacaa aaagcgttac accgttaaat aacgctgcga
    28921 gtacttcgaa acgtttataa ccaaatgttt ttgtagttgt ggcattcttt tcagcataaa
    28981 taaatgcgac aagtgcaaca cctaatgaga atgtgtcgct aaacatatgg ataccgtcag
    29041 atagtaatgc caagctgtta gcaaggagac cgccgatgat ttcgataaac atatatagac
    29101 cgattattaa aaacgatata aacaatactt tcttattatt tgtagttaca tgactatgca
    29161 tatggtcatg atgatgatgt gaatgagaca tattataacc cactttcttt aggatgattc
    29221 gcgtgatgta tggcttgctt taacatagtt gctacgtgga tgtcatctaa tgaataaatc
    29281 attgattggc cttgtcgttt tgctttcaca agatgcacac ttttaagtaa ttttaattgg
    29341 tgcgagacat ttgattgaga taaattcaat tgatgtgaaa tgtgaccaac acttgcttcg
    29401 ctgactgata acaattccat gatacgtatt cgattgtaat cgcctaatgc cttgaaaatt
    29461 tcagttacgc gttctaatgt atctgtattt atttctgaat attgttctga cattatatga
    29521 caatcctttc atgtgaatat ttgttcatat attaatttaa tacgttccca gtagtatgtc
    29581 aattgatttt tatagttgta acaagtttta catatctcat aaagtggtat ggcatagaga
    29641 ataataaata ggagagtgtt gtaaatgaat attttggtta taggtgctaa tggcggtgta
    29701 ggttcgttac tagtgcaaca attagcaaaa gaaaatgtac catttactgc tggtgttagg
    29761 caatcagatc aacttaatgc gttaaaatca caaggtatga aagcaattct tgttgatgtt
    29821 gaaaatgatt caatagagac tttaactgag acgtttaaac cattcgataa agttatcttt
    29881 tcagtaggtt ctggagggaa cacgggtgca gataaaacaa ttattgtcga tttagatggt
    29941 gctgttaaat caatgattgc gagtaaagag gccaatatta aacactatgt catggtttca
    30001 acatatgatt cgagacgtca ggcttttgat gatagcggtg atttaaagcc atatacaata
    30061 gctaaacatt acgctgatga ctatttaaga cgttcaggtt tgaattatac aattttacat
    30121 ccaggggcac ttacaaatgc agcaggatct ggaaagatag aagctgcaca atattttgat
    30181 ggtaaaggtg aaattcctag agaagatgtt gcgacagtat taaaagaaat tgtcacatcc
    30241 aatcatttta atcaccaaga gtttcaaatt attagtggtg atcaggacat taaagatgct
    30301 ttaactcaat ttgaaaatga aacggattaa taaggatagt aaatatgatt tgaagcgggt
    30361 tactttttcg taactcgtta ttttttgaaa atgatgaatt ataagtatga ataagtacgt
    30421 attgtctatg cgttttacaa agaatttgaa caatgtatgg cacaactatt aaagttgttt
    30481 attaattggt ataatgggcg agtaaattta aagggaaata tgtacaaatg taagagagat
    30541 ataatctcta tgctataaat ttttgaaaat gttaaagtac tgtaaattta agtgaagcgc
    30601 tttaattggc agtactgcaa tgttagtaaa tatggtacaa tcactaatag ttaatttgaa
    30661 atgtggaagt taataatacg atgtaaatga gaacaaaatc aaaatataat ggaacaacaa
    30721 catcagagtt ctattgctta cttaatcaca atggaggtgc aacaatgcca ttatttttac
    30781 aaccaatttt aaaaacaaaa ttatggggcg gtcaacgtct aagtgagttt ggatatcaat
    30841 tagacaatga tacaactggg gaatgttggt gtgtgtcagc acatccaaat ggtacgagcg
    30901 agattattaa tggaccatat caaggtcaaa cattagaccg tatttggtca gaacatcgtg
    30961 aattgtttgg tgatttccca agcaaagatt ttccgcttct aactaaaata gtggatgcaa
    31021 gagaatcact ttctattcat gtgcaccctg ataattctta tgcttatgag catgaaaacg
    31081 ggcaatatgg caaatctgaa tgttggtata ttatagatgc agaagaagat gcagaaatag
    31141 ttatagggac attagcagag tctagagaag aagttgcgaa tcatgttcaa cacggaacga
    31201 tagagtcgat acttagatat attaaagtaa aacctggaga attctatttt attccagcag
    31261 gaacagtaca tactatttct tcaggaatat tagcatacga aacgatgcaa tcgtcagaca
    31321 ttacatatag actttatgat ttcaatcgtc aagataatca atataatgat agaccgttaa
    31381 atattgaaaa agctttagac gttattcagt acaatgcacc attacctaat attttgcctg
    31441 aaagcgaaat tattgaaaac cataagtgta cacacattgt atcgaatgat ttctttacat
    31501 tggttaaatg ggaaatttct ggcacgttaa attatatgaa gcctagagag ttctgtttag
    31561 ttacagtgtt ggaaggcgaa gggcaaatga ttgtcgatgg tgaaattttc aaactgacta
    31621 ctggtacaaa ctttattttg acttctgaag atttggatag tgtctttgaa ggtgatttca
    31681 cattgatgat tagctatgtg taatagtgta ataacaattt aggagtgaat gatttgaagc
    31741 aatttctata tattgcgtta gtatgtggtg tgatagcagg tcttggtgct ttcttacata
    31801 taccgcagta tccgagcatg acaattccac gtatagtagc tattttagga attatcagtg
    31861 ctatgttgac ttttaaagac aagcaaatca gcgcctcatt aaagtttagc gcattgttaa
    31921 ttaatgtgct gccattatgc ggtacctttg tagcttcaaa ttaattgtcg atagcgcgtt
    31981 tgaatataat acagtgcatt aacagaagaa atatgattgt gttaatgaca ttgatgaaaa
    32041 taaagcacat caacataacc tctcacttgc atagtagtga gggggttttt gttggtttat
    32101 ataaaatgaa gtaattaata tttaatgaaa attaagacat gataaaattc taacggcagt
    32161 atagatattt aaaaatgtgt atcaatggcg ctatagagat aagcaagcat agtggtatca
    32221 tatataaatt tgtaattttt attgcatagt gtatgcaagc atgtttttta tatgcaaaat
    32281 aggtagctaa atatgttaaa ctgtttgtaa caatttttat cattaagaca gatataataa
    32341 ataaaatata gaaaactgat taaaaggtat acagttgctc gtatgtgtgt ccaactgtat
    32401 ttagagtcgc acttgttgta aagcaaaata gttcgactta cttagaagtg aaaacgataa
    32461 gggtctggga cgtaaattaa tgtcctgggc gctacaatgt tataggaaat gcgattaaaa
    32521 tcgcagtgaa caaagggtat acagttgttc gcatacgcgt ccaactgcat aagaaacact
    32581 aaaagaatgt aattcatagt gtttctaaaa tttattcagg ttaaggagta ttaagatgac
    32641 agcagaaacg aattattttt ggttgaattg tggatataac cgttggaatc ataatgaacc
    32701 gttagtagga caaacagctt tatttgaatc aggcgcacat tttaacccat ctcaaggatt
    32761 tagagcgttt aaaaaggcta aagtaggaga tcaagtcatc ttttatcaag ttcagacgga
    32821 tactggacta cttggatgtg gtgaaattat tagtgttgaa acaggtgcac aaaataaaat
    32881 acgtgtacag tttcgtttta atgaacaatt gaaaccatta acagcagatt atttaaagcg
    32941 tagtgaagct ttggaatttc gtataagtaa tatgaaagag acattgttca atcaaatcac
    33001 tgcagaagag tttgatttaa taagtggtct tggtaaaggc gaaattaaga taccgcgtta
    33061 tttcttttta gctgaaactg aagaatttga acctggaaac caatatacga tttatacgca
    33121 cacgtacaac ggtattaaac gtaatggtta tcatttttat actcagttag aagaaggcga
    33181 caatattatt ttttataatc gaacgaaaaa tcaatctgtc gttggaattg gagaagtatc
    33241 aaagcatatt catgaaaaac caccaattcc tggtagaaca aatagtacag tgattgaagt
    33301 gtcttatgag aaagacatta caccgattac attaagtaca ttaaataagc atcctaaatt
    33361 gaaaaactta tatttccttc aagaaaatgc aaaacaggct attgcaagta tgtcacaagc
    33421 acagtatgat gcaattattg aaatgagcga taataatgga ttgaaatctc catttgaaat
    33481 ggttcaaaaa ccagatatgc ttgaaagtga aaaagaagaa gcgttaaagc catttatttt
    33541 acttgttgta gatcgaaaag aagaaggctt aaaagcggct aatgacttat tgcaaaaagc
    33601 gaatgcaaat cctgttataa caacaggtca tcctgatttt tcagaagata tgctttatgg
    33661 caagtattta ccaaatgaaa caggggcatt atactatcgt gaagggttta ttactcaatt
    33721 aatgcctaaa aaggataaaa gttatttagt tattgataat tttaatcgta tagatacaga
    33781 cattttccaa acgtatatta atgtgttaga aggctatgaa gtaacattac cacgttataa
    33841 taaagacggt aatatgatta aatggtcacg acaaaaagat tctttctatt atttcaatcc
    33901 taattggcat attgtaggta ttacatatga cagtttagag gaaattaagg agaaatattc
    33961 agagcagttt ttaaaataca cacgtatagt caaagtcaaa catgactaat gatatatggt
    34021 agtttttgaa aagaagtaag gataggatta aaaattgcat aattgcatat ttgaataacc
    34081 gcataaagaa aaaatgacgg gtaatcaacc ataagagcta attaagagct tatgatagat
    34141 tactcgtttt tattttaata aataataaag acagtatatg aatataaaag aatttatgtt
    34201 acagaattaa aactttatat gagttttcca gtggtatgtt gaataagacg aatgaccaaa
    34261 cgctaagtta ataataagaa ataagattac actgtagatt gagaaattta aagtaaataa
    34321 aaaatacatt atacaataaa tataaaactg aaaaataaat tttatggcaa tttcgtgtcg
    34381 cgaggactta tcaagtgaca accttgttta aatgttttta aattaggaaa aatgactata
    34441 agtactatta aaggagcgaa taaattatgc caatcgtata ttatgatgga aactgtatat
    34501 attgttataa ctatgtcatt tggttaattc aacatgacct accaagaaat tatcaatttg
    34561 ccacgttaaa aggtgaagtt ggccaacaat tttttaaaca acatccagaa gcggcaaata
    34621 aaaatagtgt gattttacag aaaggtgatc gtatatattt tgaatcgcag gcgattatta
    34681 aactaatcac agcactacct aatcgaacta aattattggg cgtaggttta tggcttgtac
    34741 ctaaaccagt ccgtaatttc ggttatcgaa tgtttgctaa taatagaaat agaatgtgga
    34801 aaacaacatg gcaccaacca aatgattatg aaaaatcgtt ctttttagat gataatgcga
    34861 aagtaaaact tactgattga taaaacatac ttgctaattg ataatggata tattagatga
    34921 tgaattaaaa tttaggcatt taaatagcgg aacactttac ggttagatta gaataattat
    34981 aaaaaagaga gcaaaaacac tttacagatt agaattatta taatataata attaatataa
    35041 acaagcaaga cgtagacaat tttaaggagt gtattaaata tgagtaatca acaagatgtt
    35101 gtaaaagaat tgaatcaaca agtagcaaac tggacagtag cttacacaaa gctacacaat
    35161 ttccactggt atgtgaaagg gcctaacttc ttctcattac acgttaaatt tgaagaatta
    35221 tataatgaag caagccaata tgtagacgaa ttagctgaaa gaattttagc ggtaggagga
    35281 aaccctgtag gtacattaac tgaatgctta gagcaatcaa ttgttaaaga agctgcgaaa
    35341 ggttactctg cagaacaaat ggttgaagaa ttatcacaag acttcacaaa tatctcaaaa
    35401 caattagaaa acgcaatcga aattgctggt aatgctggcg atgatgtatc agaagatatg
    35461 tttataggta tgcaaacatc agtagataaa cataattgga tgtttaaatc ttacttaagc
    35521 taatctacag ataagtaata atataaataa aagccaacac ataaatgggg atacgcccag
    35581 aatgtgttgg ctttttgagt ttaatcgcat tgttaaagag caattaaagt caatttaaat
    35641 aagttactct gcgatttcta aagcgatttc catcatttgt gtaaatgaat tttgacgttc
    35701 ttcaggtgta gtagcttcgt cacgtaaaat atgatcactt actgtgaaaa taccaagtgc
    35761 ttttttacca gcatgaatcg catttaaata taaaccagct gattccattt cgatacctaa
    35821 aatacccatt tttttccaag catcattgaa tgttggatcg gcattgtaaa atgtatcaga
    35881 agataatacg ttacctacgt gtgtagtagc accgatttgg tcagcgacat ttttagcttt
    35941 agttactaac tcgaagtcag cgataggcgc gaaatgacct ggaatattgt attgatctac
    36001 ataatttgaa ttagttgatg cagcttgtgc aataataaca tcgtataagt taacattttc
    36061 ttgtaatgcg ccacaagaac cgatacgaat gattgtatct acatcaaaga agttgtataa
    36121 ctcatatgag taaataccaa tacttggaat acccatacca gaacccatta cagaaacttc
    36181 tttaccttta tatgtaccag tgtaaccaaa catgttacgt acatcgttaa attgttcaac
    36241 attttctaaa aaattatcag caatatattt tgcacgtagc ggatcgccag gcattaatac
    36301 tgttttagca atttttactc catttggttg aatatgtggt gtaccttttg tcatttgtta
    36361 tcttctcctt aatattcatt aataatttta agataacatt tgtttccctt ttttgcgaat
    36421 ttttgtacta tagaattaaa gaattataaa aatataatcc tgattagctt gttgtcaagt
    36481 catcgttcat aatgaagata tcacgttcaa ttgtattgtt tgtttatggg aaatgaatta
    36541 atgtaatagt atatgtatgc ggttacataa aaagcgaaca tctaacctga tatttaaatg
    36601 aacctgacgc tcaatcaact aatttacaac cgtattttta taatcaacca taaaggagga
    36661 gatagaaaat gaatagtgca aaattgattg atcacacttt attgaagcct gagtcaacac
    36721 gtacgcaaat cgatcaaatc atcgatgaag cgaaagcata ccattttaaa tctgtatgtg
    36781 tgaatccaac gcatgttaaa tatgcagcag agcgactagc tgattcagag gtgttagttt
    36841 gtacggtaat aggattccca ttaggtgcat cgacaactgc gacgaaagca tttgaaacag
    36901 aagatgcgat tcaaaatggt gcagatgaaa ttgacatggt catcaacatc ggcgcattaa
    36961 aagatggacg ttttgatgat gtacaacaag acattgaagc agtggt
//
LOCUS       DALAGD010000012        36847 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.012, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000012 DALAGD010000000
VERSION     DALAGD010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 36847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36847
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.012"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            124..785
                     /locus_tag="O2701_000445"
                     /pseudo
     CDS             124..785
                     /locus_tag="O2701_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1356..1757
                     /locus_tag="O2701_000446"
     CDS             1356..1757
                     /locus_tag="O2701_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCZ4292356.1"
                     /translation="MKKVNNDTVFGILQLETLLGDINSIFSEIESEYKMSREEILILL
                     TLWQKGSMTLKEMDRFVEVKPYKRTRTYNNLVELEWIYKERPVDDERTVIIHFNEKLQ
                     QEKVELLNFISDAIASRATAMQNSLNAIIAV"
     gene            complement(1877..2440)
                     /locus_tag="O2701_000447"
     CDS             complement(1877..2440)
                     /locus_tag="O2701_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCZ4292357.1"
                     /translation="MKLERILPFSKTLIKQHITPESIVVDATCGNGNDTLFLAEQVPE
                     GHVYGFDIQDLALENTRDKVKDFNHVSLIKDGHENIEHHINDAHKGHIDAAIFNLGYL
                     PKGDKSIVTKPDTTIQAINSLLSLMSIEGIIVLVIYHGHSEGQIEKHALLDYLSTLDQ
                     KHAQVLQYQFLNQRNHAPFICAIEKIS"
     gene            complement(2437..3390)
                     /locus_tag="O2701_000448"
     CDS             complement(2437..3390)
                     /locus_tag="O2701_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500380.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCZ4292358.1"
                     /translation="MGNHFQYAFENKRYHTWNYHLKNKFGQKIFKVALDGGFDCPNRD
                     GTVAHGGCTFCSAAGSGDFAGNRADSIAVQFKEIKEKMHEKWHEGKYIAYFQAFTNTH
                     APVEVLKEKFEPVLKEPGVVGLSIGTRPDCLPDDVVEYLADLNQRTYLWVELGLQTIH
                     QSTSDLINRAHDMKTYYDGVAKLRKHNINVCTHIINGLPGEDYDMMMATAKEVAQMDV
                     QGIKIHLLHLLKGTPMVKQYDKGLLTFMTQEEYTNLVVDQLEVIPPEMIVHRITGDGP
                     IDIMVGPMWSVNKWEVLNGIDAELARRNSYQGLRYKSKVKQ"
     gene            3500..4681
                     /locus_tag="O2701_000449"
     CDS             3500..4681
                     /locus_tag="O2701_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ4292359.1"
                     /translation="MNIPKSVWWLVIGMALNITGSSFLWPLNTIYMKQELGKSLTVAG
                     LVLMINSFGMVIGNLLGGSLFDKLGGYKTILIGTFTCLCSTTLLNFFHGWPWYAVWLV
                     MLGFGGGMIIPAIYAMAGAVWPNGGRQTFNAIYLAQNIGVAVGAAMGGFVAEFSFNYI
                     FLANLIMYVVFALVAVTQFNIEINAKVKYPTHLDITGKKNKARFISLVLICAMFAICW
                     VAYIQWESTIASFTQSINISMAQYSVLWTINGIMILVAQPLIKPILYLLKGNLKKQMF
                     VGIIIFMLSFFVTSFAENFTIFVVGMIILTFGEMFVWPAVPTIANQLAPDGKQGQYQG
                     FVNSAATVGKAFGPFLGGVLVDAFNMRMMFIGMMLLLVFALILLMVFKENNTQPKKID
                     A"
     gene            4969..7386
                     /locus_tag="O2701_000450"
     CDS             4969..7386
                     /locus_tag="O2701_000450"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCZ4292360.1"
                     /translation="MLNYNHNQIEKKWQDYWDENKTFKTNDNLGQKKFYALDMFPYPS
                     GAGLHVGHPEGYTATDIISRYKRMQGYNVLHPMGWDAFGLPAEQYALDTGNDPREFTK
                     KNIQTFKRQIKELGFSYDWDREVNTTDPEYYKWTQWIFIQLYNKGLAYVDEVAVNWCP
                     ALGTVLSNEEVIDGVSERGGHPVYRKPMKQWVLKITEYADQLLADLDDLDWPESLKDM
                     QRNWIGRSEGAKVSFDVDNTEGKVEVFTTRPDTIYGASFLVLSPEHALVNSITTDEYK
                     EKVKAYQTEASKKSDLERTDLAKDKSGVFTGAYATNPLSGEKVQIWIADYVLSTYGTG
                     AIMAVPAHDDRDYEFAKKFDLPIIEVIEGGNVEEAAYTGEGKHINSGELDGLENEAAI
                     TKAIQLLEQKGAGEKKVNYKLRDWLFSRQRYWGEPIPVIHWEDGTMTTVPEEELPLLL
                     PETDEIKPSGTGESPLANIDSFVNVVDEKTGMKGRRETNTMPQWAGSCWYYLRYIDPK
                     NENMLADPEKLKHWLPVDLYIGGVEHAVLHLLYARFWHKVLYDLAIVPTKEPFQKLFN
                     QGMILGEGNEKMSKSKGNVINPDDIVQSHGADTLRLYEMFMGPLDAAIAWSEKGLDGS
                     RRFLDRVWRLMVNEDGTLSSKIVTTNNKSLDKVYNQTVKKVTEDFETLGFNTAISQLM
                     VFINECYKVDEVYKPYIEGFVKMLAPIAPHIGEELWSKLGHEESITYQPWPTYDEALL
                     VDDEVEIVVQVNGKLRAKIKIAKDTSKEEMQEIALSNDNVKASIEGKDIMKVIAVPQK
                     LVNIVAK"
     gene            7408..7719
                     /locus_tag="O2701_000451"
     CDS             7408..7719
                     /locus_tag="O2701_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCZ4292361.1"
                     /translation="MKSITTDELKNKLLESKPVQIVDVRTDEETAMGYIPNAKLIPMD
                     TIPDNLNSFNKNEIYYIVCAGGVRSAKVVEYLEANGIDAVNVEGGMHAWGDEGLEIKS
                     I"
     gene            8045..14605
                     /gene="sasC"
                     /locus_tag="O2701_000452"
     CDS             8045..14605
                     /gene="sasC"
                     /locus_tag="O2701_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored repetitive surface protein SasC"
                     /protein_id="HCZ4292362.1"
                     /translation="MNLLKKNKYSIRKYKVGIFSTLIGTVLLLSNPNGAQALTTDNNV
                     QSDTNQATPVNSQDKDVANNRGLANSAQNTPNQSATTNQATNQALVNHNNGSIVNQAT
                     PTSVQSSTPSAQNNNHTDGNTTATETVSNANNNDVVSNNTALNVPTKTNENGSGGHLT
                     LKEIQEDVRHSSNKPELVAIAEPASNRPKKRSRRAAPADPNATPADPAAAAVGNGGAP
                     VAITAPYTPTTDPNANNAGQNAPNEVLSFDDNGIRPSTNRSVPTVNVVNNLPGFTLIN
                     GGKVGVFSHAMVRTSMFDSGDNKNYQAQGNVIALGRIHGTDTNDHGDFNGIEKALTVN
                     PNSELIFEFNTMTTKNGQGATNVIIKNADTNDTIAEKTVEGGPTLRLFKVPDNVRNLK
                     IQFVPKNDAITDARGIYQLKDGYKYYSFVDSIGLHSGSHVFVERRTMDPTATNNKEFT
                     VTTSLKNNGNSGASLDTNDFVYQVQLPEGVEYVNNSLTKDFPSNNSGVDVNDMNVTYD
                     AANRVITIKSTGGGTANSPARLMPDKILDLRYKLRVNNVPTPRTVTFNETLTYKTYTQ
                     DFINSAAESHTVSTNPYTIDIIMNKDALQAEVDRRIQQADYTFASLDIFNGLKRRAQT
                     ILDENRNNVPLNKRVSQAYIDSLTNQMQHTLIRSVDAENAVNKKVDQMEDLVNQNDEL
                     TDEEKQAAIQVIEEHKNEIIGNIGDQTTDDGVTRIKDQGIQTLSGDTATPVVKPNAKK
                     AIRDKATKQREIINATPDVTEDEIQDALNQLATDETDAIDNVTNATTNADVETAKNNG
                     INTIGAVVPQVTHKKAARDAINQATATKRQQINSNREATQEEKNAALNELTQATNHAL
                     EQINQATTNADVDNAKGDGLNAINPIAPVTVVKQAARDAVSHDAQQHIAEINANPDAT
                     QEERQAAIDKVNAAVTAANTNILNANTNADVEQVKTNAIQGIQAITPATKVKTDAKNA
                     IDKSAETQHNTIFNNNDATLEEQQAAQQLLDQAVATAKQNINAADTNQEVAQAKDQGT
                     QNIVVIQPATQVKTDTRNVVNDKAREAITNINATTGATREEKQEAINRVNTLKNRALT
                     DIGVTSTTAMVNSIRDDAVNQIGAVQPHVTKKQTATGVLNDLATAKKQEINQNTNATT
                     EEKQVALNQVDQELATAINNINQADTNAEVDQAQQLGTKAINAIQPNIVKKPAALAQI
                     NQHYNAKLAEINATPDATNDEKNAAINTLNQDRQQAIESIKQANTNAEVDQAATVAEN
                     NIDAVQVDVVKKQAARDKITAEVAKRIEAVKQTPNATDEEKQAAVNQINQLKDQAINQ
                     INQNQTNDQVDTTTNQAVNAIDNVEAEVVIKPKAIADIEKAVKEKQQQIDNSLDSTDN
                     EKEVASQALAKEKEKALAAIDQAQTNSQVNQAATNGVSAIKIIQPETKVKPAAREKIN
                     QKANELRAKINQDKEATAEERQVALDKINEFVNQAMTDITNNRTNQQVDDTTSQALDS
                     IALVTPDHIVRAAARDAVKQQYEAKKREIEQAEHATDEEKQVALNQLANNEKRALQNI
                     DQAIANNDVKRVETNGIATLKGVQPHIVIKPEAQQAIKASAENQVESIKDTPHATVDE
                     LDEANQLISDTLKQAQQEIENTNQDAAVTDVRNQTIKAIEQIKPKVRRKRAALDSIEE
                     NNKNQLDAIRNTLDTTQDERDVAIDTLNKIVNTIKNDIAQNKTNAEVDRTETDGNDNI
                     KVILPKVQVKPAARQSVGVKAEAQNALIDQSDLSTEEERLAAKHLVEQALNQAIDQIN
                     HADKTAQVNQDSINAQNIISKIKPATTVKATALQQIQNIATNKINLIKANNEATDEEQ
                     NIAIAQVEKELIKAKQQIASAVTNADVAYLLHDEKNEIREIEPVINRKASAREQLTTL
                     FNDKKQAIEANIQATVEERNSILAQLQNIYDTAIGQIDQDRSNAQVDKTASLNLQTIH
                     DLDVHPIKKPDAEKTINDDLARVTALVQNYRKVSNRNKADALKAITALKLQMDEELKT
                     ARTNADVDAVLKRFNVALSDIEAVITEKENSLLRIDNIAQQTYAKFKAIATPEQLAKV
                     KVLIDQYVADGNRMIDEDATLNDIKQHTQFIVDEILAIKLPAEATKVSPKEIQPAPKV
                     CTPIKKEETHESRKVEKELPNTGSEGMDLPLKEFALITGAALLARRRTKNEKES"
     gene            complement(14722..15990)
                     /locus_tag="O2701_000453"
     CDS             complement(14722..15990)
                     /locus_tag="O2701_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCZ4292363.1"
                     /translation="MYQTIIIGGGPSGLMAAVAASEQSSSVLLIEKKKGLGRKLKISG
                     GGRCNVTNRLPYAEIIKNIPGNGKFLYSPFSIFDNESIIDFFESRGVKLKEEDHGRMF
                     PVSNKAQDVVDTLVTTIERQHVTIKEEEAVSRIEVNTDQTFTVHTQNNSYESHSLVIA
                     TGGTSVPQTGSTGDGYKFAQDLGHTITELFPTEVPITSAEPFIKSNRLKGLSLKDVEL
                     SVLKKNGKKHISHQMDMLFTHFGISGPAALRCSQFVYKEQKNQKTQHISMAIDAFPEL
                     NHEQLKQHITSLLSDTPDKIIKNSLHGLIEERYLLFMLEQAGIDENTTSHHLSNQQLN
                     DLVNMFKGFVFKVNGTLPIDKAFVTGGGVSLKEIQPKTMMSKLVPGLFLCGEVLDIHG
                     YTGGYNITSALVTGHVAGLYAGHYSHASME"
     gene            16397..18058
                     /locus_tag="O2701_000454"
     CDS             16397..18058
                     /locus_tag="O2701_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCZ4292364.1"
                     /translation="MSESKEMVRGTFLITISILITKVLGVLFIIPFNYLIGGQENMAP
                     FTYAYAPYNIAIAVATAGVPLAASKYVAKYNAIGAYKVSQKFYKSSFIVMSITGVLGF
                     LVLYFLAPYISELTLARNIHDKNGWSVDDITWIIRIISMVVIFIPVLATWRGIFQGYK
                     SMGPTAVSEVTEQIARVIFILIGSYLVLNVFDGSILLANGIATFAAAVGAIIGIFTLW
                     YYWRKRKHNIDRMVESDYTDIDVSYGKMYKEIIAYSIPFVIVSLNYPLFNLVDQFTHN
                     GALSLVGIPSQLQDIFFNMLNMSTNKIVMIPTSLSAGFAVSLIPYITKTFAEGRLHEM
                     HHQIRTSIGVLMFITVPASIGIMALAQPLFTVFYGYDPIVLGHDPNHDGSRLLFYYAP
                     VAILISLLSVTASMLQGIDKQKLTVYVILASVVIKLALNYPLIMLFHTPGAILSTSIA
                     LLFAIGCNFYILKKYAKFKFSYSWIHFAKIFLYSFIMMLGVELVFFLANLFLEPTKLG
                     YLIIIILGVTVGILIYGTITIKTRLADEFLGEIPEKLRRRVRFLR"
     gene            18055..18750
                     /locus_tag="O2701_000455"
     CDS             18055..18750
                     /locus_tag="O2701_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCZ4292365.1"
                     /translation="MRIDKFLANMGVGTRNEVKQLLKKGLVNVNEQVIKSPKTHIEPE
                     NDKITVRGELIEYIENVYIMLNKPKGYISATEDHHSKTVIDLIPEYQHLNIFPVGRLD
                     KDTEGLLLITNDGDFNHELMSPNKHVSKKYEVISANPITEDDIQAFKEGVTLTDGKVK
                     PAILTYIDNQTSHVTIYEGKYHQVKRMFHSIQNEVLHLRRIKIADLELDSNLDSGEYR
                     LLTENDFDKLNYK"
     gene            18767..19189
                     /locus_tag="O2701_000456"
     CDS             18767..19189
                     /locus_tag="O2701_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCZ4292366.1"
                     /translation="MAKGNLFKAILGIGGAVAAVLVTRKDSRDKLKAEYNKYKQDPQS
                     YKDNAKDKATQLGTIANETIKEVKTNPKEYANRLKNNPKAFFKEEKSKFTEYDNKTDE
                     SIEKGKFDDEGGAAPNNNLRIVTEEDLKKNKNALSDKE"
     gene            19939..21348
                     /gene="sapep"
                     /locus_tag="O2701_000457"
     CDS             19939..21348
                     /gene="sapep"
                     /locus_tag="O2701_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+)-dependent dipeptidase Sapep"
                     /protein_id="HCZ4292367.1"
                     /translation="MWKEKVQQYEDQIINDLKGLLAIESVRDDAKASEDAPVGPGPRK
                     ALDYMYEIAHRDGFTTHDVDHIAGRIEAGKGNDVLGILCHVDVVPAGDGWDSNPFEPV
                     VTEDAIIARGTLDDKGPTIAAYYAIKILEDMNVDWKKRIHMIIGTDEESDWKCTDRYF
                     KTEEMPTLGFAPDAEFPCIHGEKGITTFDLVQNKLTEDQDEPDYELITFKSGERYNMV
                     PDHAEARVLVKENMTDVIQDFEYFLEQNHLQGDSTVDSGILVLTVEGKAVHGMDPSIG
                     VNAGLYLLKFLASLNLDNNAQAFVAFSNRYLFNSDFGEKMGMKFHTDVMGDVTTNIGV
                     ITYDNENAGLFGINLRYPEGFEFEKAMDRFANEIQQYGFEVKLGKVQPPHYVDKNDPF
                     VQKLVTAYRNQTNDMTEPYTIGGGTYARNLDKGVAFGAMFSDSEDLMHQKNEYITKKQ
                     LFNATSIYLEAIYSLCVEE"
     gene            21352..22200
                     /gene="dat"
                     /locus_tag="O2701_000458"
     CDS             21352..22200
                     /gene="dat"
                     /locus_tag="O2701_000458"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HCZ4292368.1"
                     /translation="MEKIFLNGEFVSPSEAKVSYNDRGYVFGDGIYEYIRVYNGKLFT
                     VTEHYERFLRSANEIGLDLNYSVEELIELSRKLVDMNQIETGAIYIQATRGVAERNHS
                     FPTPEVEPAIVAYTKSYDRPYDHLENGVNGVTVEDIRWLRCDIKSLNLLGNVLAKEYA
                     VKYNAVEAIQHRGETVTEGSSSNAYAIKDGVIYTHPINNYILNGITRIVIKKIAEDYN
                     IPFKEETFTVDFLKNADEVIVSSTSAEVTPVIKLDGEPVNDGKVGPITRQLQEGFEKY
                     IESHSI"
     gene            22750..23541
                     /locus_tag="O2701_000459"
     CDS             22750..23541
                     /locus_tag="O2701_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HCZ4292369.1"
                     /translation="MEQFYQLGWTLDSAGGASGEAYMAEQDGQKLFLKRNSNPFIAAL
                     SAEGIVPKLVWTKRIETGEVVTAQHWKNGRELSSNEMKQTRVAHLLKKIHNSRPLLSM
                     LKRMEMEPITPEIMLNKINASLSREVLTHHIVRKSLTYLEEHIPSLDSRFFTVVHGDV
                     NHNNWLLSDRDELFLVDWEGAMIADPAIDIGMLLYNYVPQQQWSEWLETYGVQESLNL
                     NKRMKWYTVIQSIGLVQWYEEQKRYKDMNTWLKFLNEVMNSNMFI"
     gene            23556..24200
                     /gene="trmB"
                     /locus_tag="O2701_000460"
     CDS             23556..24200
                     /gene="trmB"
                     /locus_tag="O2701_000460"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCZ4292370.1"
                     /translation="MRVRYKPWAEDYLKDHPELVDMDGQHAGKMTEWFDKTQPIHIEI
                     GSGMGQFITTLAAQNPHINYISMEREKSIVYKVLDKVKEMGLTNLKIICNDAIELNEY
                     FKDGEVSRIYLNFSDPWPKNRHAKRRLTYHTFLALYQQILNDEGDLHFKTDNRGLFAY
                     SLESMSQFGMYFTKINLNLHQEDDGSNILTEYEKKFSDKGSRIYRMEAKFHSQK"
     gene            24670..25512
                     /locus_tag="O2701_000461"
     CDS             24670..25512
                     /locus_tag="O2701_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCZ4292371.1"
                     /translation="MKIGDISIHYLNGGNTKMDGGAMFGVVPKPLWSKQYNANERNQI
                     NLPTHPILIQTAQYNLIIDAGIGNGKLSEKQLRNFGVDEESHIIADLANYNLTPKDID
                     YVLMTHMHFDHAAGLTDQAGHAIFENAIHVVQQDEWHEFIAPNIRSKSTYWDKNKGDY
                     SNKLILFEKHFEPVLGIKMQHSGGHSFGHTIITIESQGDKAVHMGDIFPTTAHKNPLW
                     VTAYDDYPMQSIREKERMIPYFIQQQYWFLFYHDENYFAVKYSDNGENIDAYILRETL
                     VDNN"
     gene            complement(25637..25948)
                     /locus_tag="O2701_000462"
     CDS             complement(25637..25948)
                     /locus_tag="O2701_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCZ4292372.1"
                     /translation="MTKLKYIIPTIIAVAIVIISTISIIQFINRKRYNPVKVLNEVKS
                     YFMNVKGSYIVYEPFVHPETDKYRLVYQGGITTIKNGQNIHYDFYADAYTGEVINIVE
                     R"
     gene            26035..27111
                     /locus_tag="O2701_000463"
     CDS             26035..27111
                     /locus_tag="O2701_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCZ4292373.1"
                     /translation="MNINKKVTLQRIQTLTELHGAPGFEEEVKNYMTQQMAPYVDEFI
                     ENRMGGFFGVKKSKNPNAKRVMIAAHMDEIGFMITNITKNGMIQFTNLGGVANDIWQG
                     QRLVIKNRNGDKIIGVVSNIPKHFRTGSEGAPEIKDLTLDIGAQNEDEVRERGIDIGD
                     TIVPHTPFTQLSEHRYSAKAWDNRYGCVLAIEILELLKDIELDVDLYVGANVQEEVGL
                     RGAKASAEMIDPDVAFVVDCSPANDVKGNQPLSGELGKGTLIRIKDGTMILKPVFRDY
                     LLKLVEAHDIEHQYYMSPGGTDGGEIHKANIGIPTAVIGVCARYIHSTDSVFDIRDYF
                     AARSLLSEAICNLDNNQIETLQYK"
     gene            27176..27487
                     /locus_tag="O2701_000464"
     CDS             27176..27487
                     /locus_tag="O2701_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCZ4292374.1"
                     /translation="MKQLESEQQFESLKQGATVFEFTAGWCPDCRVIEPDLPELEARY
                     PMFDFVSVDRDKFMDICIENGIMGIPSFLVYKNGELLGSYIGKERKSIEQIDAFLAQY
                     V"
     gene            27587..28444
                     /locus_tag="O2701_000465"
     CDS             27587..28444
                     /locus_tag="O2701_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1444 domain-containing protein"
                     /protein_id="HCZ4292375.1"
                     /translation="MNTFQMRDKLKERLSHLDVDFKFNREEETLRIYRTDNNKGITIK
                     LNAIVAKYEDKKEKIVDEIVYYVDEAIAQMADKTLESISSSQIMPVIRATSFDKKTKQ
                     GVPFIYDEHTAETAVYYAVDLGKSYRLIDESMLEDLKLTEQQIREMSLFNVRKLSNSY
                     TTDEVKGNIFYFINSNDGYDASRILNTAFLNEIEAQCQGEMLVAVPHQDVLIIADIRN
                     KTGYDVMAHLTMEFFTKGLVPITSLSFGYKQGHLEPIFILGKNNKQKRDPNVIQRLEA
                     NRRKFNKDK"
     gene            28473..29069
                     /locus_tag="O2701_000466"
     CDS             28473..29069
                     /locus_tag="O2701_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HCZ4292376.1"
                     /translation="MNLFYNPKYVGDVAFLQIEPVEGELNYNKKGNVVEITNEGNVVG
                     YNIFEISKDITIEEKGHIKLTDELVNVFQKRISEAGFDYKLNADLSPKFVVGYVETKD
                     KHPDADKLSVLNVNVGNDTLQIVCGAPNVEAGQKVVVAKVGAVMPSGMVIKDAELRGV
                     ASSGMICSMKELNLPNAPEEKGIMVLNDSYEIGQAFFE"
     gene            29090..32914
                     /locus_tag="O2701_000467"
     CDS             29090..32914
                     /locus_tag="O2701_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCZ4292377.1"
                     /translation="MSWFDKLFGEDNDSNDDLIHRKKKRRQESQNIDNDHDSLLPQNN
                     DIYSRPRGKFRFPMSVAYENENVEQSADTISDEKEQYHRDYRKQSHDSRSQKRHRRRR
                     NQTTEEQNYSEKRGNSKISQQSIKYKDHSHYHTNKPGTYVSAINGIEKETHKPKTHNM
                     YSNNTNHRAKDSTPDYHKESFKTSEVPSAIFGTMKPKKLENGRIPVSKPSEKVESDKQ
                     KYDKYVAKTQTSQNKQLEQEKQNDSVVKQGTASKSSDENVSSTTKSMPNYSKVDNTIK
                     IENIYASQIVEEIRRERERKVLQKRRFKKALQQKREEHKNEEQDAIQRAIDEMYAKQA
                     ERYVGDSSLNDDSDLTDNSTDASQLHTNGIENETVSNDENKQASIQNEDTNDTHVDES
                     PYNYEEVSLNQVSTTKQLSDDEVTVSNVTSQHQSALQHNVEVNDKDELKNQSRLIADS
                     EEDGATNKEEYSGSQIDDAEFYELNDTEVDEDTTSNIEDNTNRNASEMHVDAPKTQEY
                     AVTESQVNNIDKTVDNEIELAPRHKKDDQTNLSVNSLKTNDVNDNHVVEDSSMNEIEK
                     NNAEITENVQNEAAESEQNVEEKTIENVNPKKQTEKVSTLSKRPFNVVMTPSDKKRMM
                     DRKKHSKVNVPELKPVQSKQAVSERMPASQATPSSRSDSQESNTNAYKTNNMTSNNVE
                     NNQLIGHAETENDYQNAQQYSEQKPSVDSTQTEIFEESQDDNQLENEQVDQSTSSSVS
                     EVSDITEESEETTHPNNTSGQQDNDDQQKDLQSSFSNKNEDTANENRPRTNQQDVATN
                     QAVQTSKPMIRKGPNIKLPSVSLLEEPQVIESDEDWITDKKKELNDALFYFNVPAEVQ
                     DVTEGPSVTRFELSVEKGVKVSRITALQDDIKMALAAKDIRIEAPIPGTSRVGIEVPN
                     QNPTTVNLRSIIESPSFKNAESKLTVAMGYRINNEPLLMDIAKTPHALIAGATGSGKS
                     VCINSILMSLLYKNHPEELRLLLIDPKMVELAPYNGLPHLVAPVITDVKAATQSLKWA
                     VEEMERRYKLFAHYHVRNITAFNKKAPYDERMPKIVIVIDELADLMMMAPQEVEQSIA
                     RIAQKARACGIHMLVATQRPSVNVITGLIKANIPTRIAFMVSSSVDSRTILDSGGAER
                     LLGYGDMLYLGSGMNKPIRVQGTFVSDDEIDDVVDFIKQQREPDYLFEEKELLKKTQT
                     QSQDELFDDVCAFMVNEGHISTSLIQRHFQIGYNRAARIIDQLEQLGYVSSANGSKPR
                     DVYVTEADLNKE"
     gene            32938..34251
                     /locus_tag="O2701_000468"
     CDS             32938..34251
                     /locus_tag="O2701_000468"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCZ4292378.1"
                     /translation="MTHYHFVGIKGSGMSSLAQIMHDLGHEVQGSDIENYVFTEVALR
                     NKGIKILPFDANNIKEDMVVIQGNAFASSHEEIVRAHQLKLDVVSYNDFLGQIIDQYT
                     SVAVTGAHGKTSTTGLLSHVMNGDKKTSFLIGDGTGMGLPESDYFAFEACEYRRHFLS
                     YKPDYAIMTNIDFDHPDYFKDINDVFDAFQEMAHNVKKGIIAWGDDEHLRKIEADVPI
                     YYYGFKDSDDIYAQNIQITDKGTAFDVYVDGEFYDHFLSPQYGDHTVLNALAVIAISY
                     LEKLDVTNIKEALETFGGVKRRFNETTIANQVIVDDYAHHPREISATIETARKKYPHK
                     EVVAVFQPHTFSRTQAFLNEFAESLSKADRVFLCEIFGSIRENTGALTIQDLIDKIEG
                     ASLINEDSINVLEQFDNAVILFMGAGDIQKLQNAYLDKLGMKNAF"
     gene            34325..34816
                     /locus_tag="O2701_000469"
     CDS             34325..34816
                     /locus_tag="O2701_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HCZ4292379.1"
                     /translation="MDWILPIAGIIAAIAFLILCIGIVAVLNSVKKNLDYVAKTLDGV
                     EGQVQGITRETTDLLHKVNRLTEDIQGKVDRLNSVVDAVKGIGDSVQTLNSSVDRVTN
                     SITHNISQNEDKISQVVQWSNVAMEIADKWQNRHYRRGSANYKANNVATDANHSYTSR
                     VDK"
     gene            34890..36248
                     /locus_tag="O2701_000470"
     CDS             34890..36248
                     /locus_tag="O2701_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292380.1"
                     /translation="MTQQQNDKRTLKNKHTYQNEPLPNRKDFVVSFITGALVGSALGL
                     YFKNKVYQKADDLKVKEQELSQKFEERKTQLEETVAYTKERVEGFLNKSKNEQAALKA
                     QQAAIKEEASANNLSDTSQEAQEIQEAKREAQAEADKSVAVSNKESKAVALKAQQAAI
                     KEEASANNLSDTSQEAQEIQEAKKEAQAETDKSAAVSNEEPKAVALKAQQAAIKEEAS
                     ANNLSDISQEAQEVQEAKKEAQAEKDSDTLTKDASAAKVEVSKPESQAERLANAAKQK
                     QAKLTPGSKESQLTEALFAEKPVAKNDLKEIPQLVTKKNDVSETETVNIDNKDTVKQK
                     EAKFENGVITRKADEKTTNNTAVDKKSGKQSKKTTPSNKRNASKASTNKTSGQKKQHN
                     KKSSQGAKKQSSSSKSTQKNNQTSNKNSKTTNAKSSNASKTPNAKVEKAKSKIEKRTF
                     ND"
     gene            36383..36487
                     /locus_tag="O2701_000471"
     CDS             36383..36487
                     /locus_tag="O2701_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292381.1"
                     /translation="MRSFHVFMLFETSLDNNSDIAHFKLKMEKYSIIK"
BASE COUNT        13759 a         5299 c         6762 g        11027 t
ORIGIN      
        1 gaaaaaataa gacatttttc ccaacttgca cataaatgta aaattcaata aaatgaattt
       61 tctgtgttgg gtcccttcgt ataatttaat aaatactact aaactaaatt aaagaggtgc
      121 cttatgtata aaaattataa catgacccca acttacacta ccaatagaaa cttcgttaga
      181 attcctcaaa atgatatttc gcgatatgtt aatgaaattg ttgaaacgat acctgatagc
      241 gaattcgatg aattcagaca tcatcgtggt gcaacatcct atcatccaaa aatgatgtta
      301 aaaatcatct tatatgcata tactcaatct gtattttctg gtcgtagaat agagaaatta
      361 cttcatgaca gtattcgaat gatgtggtta gcccaagatc aaacactttc ttataaaact
      421 attaatcgtt ttagagtgaa tcctaatact gatgcgttaa ttgaatcttt atttattcag
      481 ttctatagtc aatgtttaaa gcaaaatctt attgatgata attcaatttt tattgatggt
      541 acaaaagtag aagctagtgc caatagatat acattggtgc ggaagaaagt attcaaaatc
      601 acgaatcgaa attgaacgag aattcaaaaa cactatatcg tgacttagtt gaagaaaaaa
      661 taataccaga gataaaagaa gatggagata gcgatttaac aatagaagaa atagatttaa
      721 ttggtagtca tttagataaa gaaatcgaag attgtgctca aattagaaaa cagacccgta
      781 aaaaagaact gaaagatgag acagtagatg ctcatctttt tttagaactt ttttaaggtt
      841 gaaaatgtat atcacatttt atacacattt gatttgtaag aaatgttttg atttatacaa
      901 atcatatctt gaaaaataac caatttagcc tcattcggtt tgatttaatt tgttaaattt
      961 aaggctaact aattaattta gtttaatcaa ttttcatgag agttatatgt aataaaaatt
     1021 caatgcgtat cttttttgaa gaaatatatg tagaattgtt gcaatttaat ggtaatattg
     1081 atatattttc tttgtatata aattataaaa ttaatatgta atagagtgat ttgttttatg
     1141 tactattatc ttatttctaa atattaactc tattgattat tggtttttat acttatttaa
     1201 ttttattcaa ctttgacaat tgaatagaaa gcaagtttat ttacacttgt agttttatgc
     1261 ataagttagc acatacaagt tttgggattg ttgggatgtt tgttaatact tgtatagtag
     1321 ctaaatatgt gattattaat tgggagatgt ttagcatgaa aaaagtaaat aacgacactg
     1381 tatttggaat tttgcaatta gaaacacttt tgggtgacat taactcaatt ttcagcgaga
     1441 ttgaaagcga atacaaaatg tctagagaag aaattttaat tttactaact ttatggcaaa
     1501 aaggttctat gacgcttaaa gaaatggaca gatttgttga agttaaaccg tataagcgta
     1561 cgagaacgta taataattta gttgaattag aatggattta caaagagcgt cctgttgacg
     1621 atgaaagaac agttattatt catttcaatg aaaagttaca acaagagaaa gtagagttgt
     1681 tgaatttcat cagtgatgcg attgcaagta gagcaacagc aatgcaaaat agtttaaacg
     1741 caattattgc tgtgtaagtt taatagcata aaaagaggtt ttcattaagt tgaaaacctc
     1801 tttttgttgt tggcattaat ttttcaaatg ttgactactc aatcctaaat tataaatagt
     1861 atagcgcagc aaatgcttaa gaaatttttt ctatggcaca aatgaatgga gcatgattac
     1921 gttggtttaa aaattgatat tgcaaaactt gcgcatgctt ttgatccaaa gtactcaagt
     1981 aatcaagcaa tgcatgcttc tcaatttgtc cttcgctatg accatgatat ataacaagta
     2041 caataatacc ttcaattgac attaatgata gcaatgaatt aatagcttgg attgtcgtgt
     2101 caggctttgt cacgatagat ttatcacctt taggcaaata acctaggtta aagatggctg
     2161 catcaatatg acctttatgt gcatcattta tatgatgttc aatattttca tgtccatctt
     2221 ttattaaaga aacatgattg aaatccttaa ctttatcacg tgtattttcc aaagctaaat
     2281 cttgaatgtc gaaaccataa acatgtcctt ctggtacttg ttcggctaaa aataaagtgt
     2341 cattgccgtt accgcaagtt gcgtctacaa caatactttc tggtgttata tgttgtttaa
     2401 taagtgtttt tgaaaaaggg agtatacgtt ctaatttcat tgcttcacct tagacttgta
     2461 acgcaagcct tgataagaat ttctacgtgc taattcagca tcgatgccat ttaatacttc
     2521 ccatttatta acactccaca ttggacctac catgatatct attggaccat caccggtaat
     2581 tcggtgaacg atcatttcag ggggaatcac ttctaattgg tccacaacta ggtttgtgta
     2641 ctcttcttga gtcataaaag ttaataaacc tttatcgtat tgttttacca tcggtgtacc
     2701 ttttaacaaa tgaagtaaat gaattttaat accttgtaca tccatttgtg caacttcttt
     2761 ggcagtagcc atcatcatgt catagtcttc gccaggtaag ccattaatga tgtgtgtaca
     2821 tacattgata ttatgcttac gtaattttgc cacaccatca taataagttt tcatatcatg
     2881 ggcacgattg attaaatcag atgttgactg atggattgtt tgtagtccta attcaaccca
     2941 taagtatgtt cgttgattca aatctgctaa atattcgaca acatcgtctg gtagacagtc
     3001 aggacgcgta ccaatagata atcccacaac acccggttct ttaagtacag gttcgaattt
     3061 ttcttttaat acttcaaccg gtgcatgtgt atttgtaaat gcctgaaaat aagcaatata
     3121 ttttccttcg tgccatttct catgcatctt ttctttaatt tctttaaatt gtactgcgat
     3181 tgaatctgca cgattacctg caaagtctcc gctacctgca gcagaacaaa atgtacatcc
     3241 accatgtgct acagtgccat cgcggttagg acagtcaaac ccgccatcca atgcaacttt
     3301 aaatattttt tgtccaaatt tattttttaa atggtaattc catgtgtgat aacgtttgtt
     3361 ttcaaaagcg tattggaaat gattgcccat atgtcatttt cctttctata aaaaaagagt
     3421 tctaagtaca gattttaaca tattttaatg ttatagtgtt tattatagtt tgacaaaaaa
     3481 gagagaggaa ctatgaaata tgaatatacc taaatcagtc tggtggctag taattggcat
     3541 ggcgttaaat attactggtt ccagtttttt gtggccttta aatacaattt atatgaaaca
     3601 agaacttgga aaaagtttaa ctgttgctgg tttagtgcta atgataaatt catttggcat
     3661 ggttattgga aacttattag gtggttcact atttgataaa ttaggtggat acaagacgat
     3721 tttaattgga actttcactt gtctttgtag tacaacgcta cttaatttct ttcacgggtg
     3781 gccttggtat gctgtatggc ttgtaatgtt agggtttggt ggcggaatga ttattcctgc
     3841 gatatacgct atggctggag cagtgtggcc aaatggcgga agacaaacgt ttaatgcgat
     3901 atacttagcg caaaatattg gtgtggctgt cggtgctgca atgggcggct ttgtcgcaga
     3961 atttagcttt aactatatct ttttagccaa tcttattatg tatgttgtgt ttgcgcttgt
     4021 cgcggtaacg caatttaata ttgaaattaa tgcgaaagtt aaatatccaa ctcatttaga
     4081 tattactggt aaaaagaata aagcaagatt tatttcatta gtactaattt gtgcaatgtt
     4141 tgcaatttgt tgggttgcat atattcaatg ggagtctaca atcgcttcat ttacacaatc
     4201 tattaatatt tcaatggcac aatatagtgt tttatggaca attaacggaa taatgatttt
     4261 agtagcacaa ccattaatta aaccgattct ctatctgtta aaaggaaact taaagaagca
     4321 aatgtttgtc ggcatcatca tttttatgtt gtcgttcttt gtcacgagtt ttgccgaaaa
     4381 ctttacaata tttgttgtcg gtatgattat tttaactttt ggagaaatgt ttgtatggcc
     4441 agcagttcca actatagcca atcagttagc gccagatggt aagcaaggac agtaccaagg
     4501 ttttgtgaat tcagctgcta cagtaggaaa agcatttggt ccatttcttg gtggtgtatt
     4561 agttgatgcg tttaatatgc gcatgatgtt tatcggtatg atgctactac ttgtatttgc
     4621 attaatatta ttaatggttt tcaaggagaa taatacgcaa cctaaaaaaa tagatgcata
     4681 atgagtaaat agaattaacg ttatagactt gaaataaatg tcgttataac ataatattaa
     4741 tttgtataat ttaatttcgt ttggagcttt tctacagaaa gctagtgatg ctgagagcta
     4801 gtgttaagga ctaaatgtaa atcgtattaa ttttaaattg aatgaatgac atctcttact
     4861 attaaaatga gtgcacaatt tttgtgaaat agggtggtaa cgcggcaaat gtcgtcccta
     4921 tgtaaataga atagttagag gtgtcttttt tattgaatag gaggaaatgt gttgaattat
     4981 aaccacaatc aaattgaaaa gaaatggcaa gactattggg acgaaaataa aacatttaaa
     5041 acaaatgata acttaggtca aaagaaattt tatgctttag acatgtttcc atatccatca
     5101 ggtgctggtt tacatgttgg acatcctgag ggctatacag caacagatat catttcaaga
     5161 tataaaagaa tgcaaggata taatgtatta catccgatgg ggtgggatgc attcggatta
     5221 ccagcagagc aatatgcttt agacactggc aacgacccac gtgaatttac aaagaaaaat
     5281 atccaaactt ttaaacgaca aattaaagaa ttagggttca gttatgattg ggatcgtgaa
     5341 gttaatacaa cagatccaga atactataaa tggacacagt ggattttcat acagttatat
     5401 aacaaaggtt tagcatacgt tgatgaagtt gcagttaact ggtgtccagc attaggcact
     5461 gttttatcta acgaagaagt gattgatggt gtctctgaac gtggtggaca tccagtttat
     5521 cgtaagccga tgaaacaatg ggtacttaaa atcacagaat atgcagatca attattagca
     5581 gatttagatg atttagattg gcctgagtct ttaaaagata tgcagcgcaa ttggattgga
     5641 cgttctgaag gggccaaagt ttcatttgat gtagataata cggaaggaaa agtagaagta
     5701 tttacgacta gaccagatac aatctatggt gcatcattct tagtcttaag tcctgaacat
     5761 gcattagtta attcaattac aacagatgaa tataaagaaa aagtaaaagc ttatcaaaca
     5821 gaagcttcta aaaagtcaga tttagaacgt acagatttag caaaagataa atcaggtgta
     5881 tttactggtg catatgcaac taatccttta tctggtgaaa aagtacaaat ttggattgct
     5941 gattatgtat tatcaacata tggtactgga gcaattatgg cagtaccagc gcatgatgac
     6001 agagattatg aatttgctaa aaagtttgat ttgccaatca ttgaagtcat cgaaggtgga
     6061 aatgttgaag aagcagcata cactggtgaa ggtaaacata ttaattctgg tgaacttgat
     6121 ggtttagaaa atgaagcggc aattactaaa gctattcaat tattagagca aaaaggtgct
     6181 ggcgaaaaga aagttaatta caaattaaga gattggttat tcagtcgtca gcgttattgg
     6241 ggcgaaccaa ttcctgtcat tcattgggaa gatggaacaa tgacaactgt tcctgaagaa
     6301 gagctaccat tgttgttacc tgaaacagat gaaatcaagc catcagggac tggtgagtct
     6361 ccactagcta atattgattc atttgtaaat gttgtagatg aaaaaacagg tatgaaagga
     6421 cgtcgtgaaa caaatacaat gccacaatgg gcaggtagtt gttggtatta tttacgttac
     6481 atcgatccta aaaatgaaaa tatgttagca gatcctgaaa aattaaaaca ttggttacct
     6541 gttgatttat atatcggtgg agtagaacat gcggttcttc acttattata tgcaagattt
     6601 tggcataaag tcctttatga tttggctatc gtacctacta aagaaccttt ccaaaaatta
     6661 tttaaccaag gtatgatttt aggagaaggt aatgagaaga tgagtaaatc taaaggaaat
     6721 gtaatcaatc ctgatgatat agtacagtct catggtgcag atactttgcg tctttacgaa
     6781 atgtttatgg gacctttaga tgctgcaatt gcatggagtg aaaaaggatt agatgggtct
     6841 cgtcgattct tagatcgcgt atggcgttta atggtaaatg aagatgggac attgagttca
     6901 aaaattgtaa ctacaaataa taaatcttta gataaagttt ataaccaaac tgttaaaaag
     6961 gtaacagaag actttgaaac attaggattt aatactgcta ttagtcaatt aatggtattt
     7021 attaatgagt gttataaagt tgatgaagtt tataaacctt acattgaagg cttcgttaaa
     7081 atgttagcac ctattgcacc acatatcggt gaagaattat ggtcaaaatt aggacatgaa
     7141 gagtctatta cgtaccaacc ttggccaact tatgacgaag cactacttgt agatgatgaa
     7201 gtagaaatcg ttgttcaagt gaatggtaaa ttgagagcta aaattaaaat tgctaaagat
     7261 acatcaaaag aagaaatgca agaaattgcc ttatctaatg acaatgttaa agcgagtatt
     7321 gaaggtaaag acatcatgaa agtcatcgct gttcctcaaa aattagtcaa tattgtagct
     7381 aaataatgtt ttaaggagga ctttgaaatg aagtcaatta ctacagatga attaaaaaat
     7441 aaacttttag aatctaaacc agttcaaatt gttgatgttc gtactgatga agaaacagca
     7501 atgggatata ttcctaatgc aaagttaatt ccaatggata ccattccgga taatttaaat
     7561 tcatttaata aaaatgaaat atattatatt gtatgtgctg gtggagttcg aagcgctaaa
     7621 gttgtagaat atttagaggc aaatggcatt gatgccgtaa atgtcgaagg cggcatgcac
     7681 gcatggggcg atgaaggttt ggaaataaaa agtatttaaa gtagtgacat aatttaaaat
     7741 aatattacat ttgtaatgac accaagtaac gtttcggttg cttggtgttt tttggtatga
     7801 attactttct gttacaaaac aatctaaagc gttcttgtta tgttttatta agattttaat
     7861 tacaaaacgg aaactaaatt gtaataaaat aaaactttat tttataaaat gatgatgata
     7921 aaattgagtg aacttaaaat attgtacaaa ataatatagc tataaatata atatgaggga
     7981 gcgtatattt ttagcataat tcttaacaac acagcagaga acagacaacc aggaggaaaa
     8041 tgaaatgaat ttgttaaaga aaaataaata tagtattagg aagtataaag taggcatatt
     8101 ctctacttta atcggaacag ttttattact ttcaaaccca aatggtgcac aagccttaac
     8161 tacggataat aatgtacaaa gcgatactaa tcaagcaaca cctgtaaatt cacaagataa
     8221 agatgttgct aataatagag gtttagcaaa tagtgcgcag aatacaccta atcaatctgc
     8281 aacaaccaat caagcaacga atcaagcatt ggttaatcat aataatggta gtatagtaaa
     8341 tcaagctacg ccaacatcag tgcaatcaag tacgccttca gcacaaaaca ataatcatac
     8401 agatggcaat acaacagcaa ctgagacagt gtcaaacgct aataataatg atgtagtgtc
     8461 gaataatacc gcattaaatg taccaactaa aacaaatgaa aatggttcag gaggacatct
     8521 aactttaaag gaaattcaag aagatgttcg tcattcttca aataaaccag agctagttgc
     8581 aattgctgaa ccagcatcta atagaccgaa aaagagaagt agacgtgcgg caccggcaga
     8641 tcctaatgca actccagcag atccagcggc tgcagcggta ggaaacggtg gtgcaccagt
     8701 tgcaattaca gcgccatata cgccaacaac tgatcctaat gccaataatg caggacaaaa
     8761 tgcacctaac gaagtgctgt catttgatga caatggtatt agaccaagta ccaaccgttc
     8821 tgtgccaaca gtaaacgttg ttaataactt gccgggcttc acactaatca atggtggcaa
     8881 agtaggggtg tttagtcatg caatggtaag aacgagcatg tttgattcag gagataataa
     8941 gaactatcaa gcacaaggaa atgtaattgc attaggtcgt atacatggaa ctgatacgaa
     9001 tgaccatggc gattttaatg gtatcgagaa agcattaaca gtaaatccga attctgaatt
     9061 aatctttgaa tttaatacaa tgactactaa aaacggtcaa ggcgcaacaa atgttattat
     9121 caaaaatgct gatactaatg atacgattgc tgaaaagact gttgaaggcg gtccaacttt
     9181 gcgtttattt aaagtacctg ataatgtgag aaatctcaaa attcaatttg tacctaaaaa
     9241 tgacgcaata acagatgcgc gtggcattta tcaactaaaa gatggttaca aatactatag
     9301 ctttgttgac tctatcggac ttcattctgg gtcacatgtt tttgttgaaa gacgaacaat
     9361 ggatccaaca gcaacaaata ataaagagtt tactgtaaca acatcattaa agaataatgg
     9421 taattctggt gcttctctag atacaaatga ctttgtatat caagttcaat tacctgaagg
     9481 tgttgaatat gtgaacaatt cattgactaa agattttcca agtaacaatt caggcgttga
     9541 tgttaatgat atgaatgtta catatgatgc agcaaatcgt gtgataacaa ttaaaagtac
     9601 tggaggaggt acagcaaact ctccggcacg acttatgcct gataaaatac tcgatttaag
     9661 atataaatta cgtgtaaata atgtgccgac accaagaaca gtaacattta acgagacatt
     9721 aacgtataaa acatatacac aagatttcat taattcagct gcagaaagtc atactgtaag
     9781 tacaaatcca tatactatcg atatcatcat gaataaagat gcattacaag ccgaagttga
     9841 cagacgtatt caacaagctg attatacatt tgcgtcatta gatatcttta atggtctgaa
     9901 acgacgcgca caaacgattt tagatgaaaa tcgtaacaat gtaccattaa ataaaagagt
     9961 ttctcaagca tatattgatt cattaactaa tcaaatgcaa catacgttaa ttcgaagtgt
    10021 tgatgctgaa aatgcagtta ataaaaaagt tgaccaaatg gaagatttag ttaatcaaaa
    10081 tgatgaattg acagatgaag aaaaacaagc agcaatacaa gttatcgagg aacataaaaa
    10141 tgaaataatt ggtaatattg gtgaccaaac gactgatgat ggcgttacta gaatcaaaga
    10201 tcaaggtata cagaccttaa gtggggatac tgcaacaccg gttgttaaac caaatgctaa
    10261 aaaagcaata cgtgataaag caacgaaaca aagggaaatt atcaatgcaa caccagatgt
    10321 tactgaagac gagattcaag atgcactaaa tcaattagct acggatgaaa cagatgctat
    10381 tgataatgtt acgaatgcta ctacaaatgc tgacgttgaa acagctaaaa ataatggcat
    10441 caatactatt ggagcagttg ttcctcaagt aactcataaa aaagctgcaa gagatgcaat
    10501 taaccaagca acagcaacga aaagacaaca aataaatagt aatagagaag caactcagga
    10561 agagaaaaat gcagcattga acgaattaac tcaagcaacc aaccatgctt tagaacaaat
    10621 caatcaagca acaacaaatg ctgatgttga taacgccaaa ggagatggtc taaatgccat
    10681 taatccaatt gctcctgtaa ctgttgttaa gcaagctgca agggatgccg tatcacatga
    10741 tgcacaacaa catatcgcag agatcaatgc taatcctgat gcgactcaag aagaaagaca
    10801 agcagcaatt gacaaagtga atgctgctgt aactgcagca aacacaaaca ttttaaacgc
    10861 taataccaat gctgatgttg aacaagtaaa gacaaatgcg attcaaggaa tacaagcaat
    10921 tacaccagct acaaaagtaa aaacagatgc aaaaaatgcc atcgataaaa gtgcggaaac
    10981 gcaacataat acgatattta ataataatga tgcgacgctc gaagaacaac aagcagcaca
    11041 acaattactt gatcaagctg tagccacagc gaagcaaaat attaatgcag cagatacgaa
    11101 tcaagaagtt gcacaagcaa aagatcaggg cacacaaaat atagtagtga ttcaaccggc
    11161 aacacaagtt aaaacggata ctcgcaatgt tgtaaatgat aaagcgcgag aggcgataac
    11221 aaatatcaat gctacaactg gcgcgactcg agaagagaaa caagaagcga taaatcgtgt
    11281 caatacactt aaaaatagag cattaactga tattggtgtg acgtctacta ctgcgatggt
    11341 caatagtatt agagacgatg cagtcaatca aatcggcgca gttcaaccgc atgtaacgaa
    11401 gaaacaaact gctacaggtg tattaaatga tttagcaact gctaaaaagc aagaaattaa
    11461 tcaaaacaca aatgcaacaa ctgaagaaaa gcaagtggct ttaaatcaag tggatcaaga
    11521 gttagcaacg gcaattaata atataaatca agctgataca aatgcggaag tagatcaagc
    11581 gcaacaatta ggtacaaaag caattaatgc gattcagcca aatattgtta aaaaacctgc
    11641 agcattagca caaatcaatc agcattataa tgctaaatta gctgaaatca atgctacacc
    11701 agatgcaacg aatgatgaga aaaatgctgc gatcaatact ttaaatcaag acagacaaca
    11761 agctattgaa agtattaaac aagctaacac aaatgcagaa gtagaccaag ctgcgacagt
    11821 agcagagaat aatatcgatg ctgttcaagt tgatgtagta aaaaaacaag cagcgcgaga
    11881 taaaatcact gctgaagtgg cgaagcgtat tgaagcggtt aaacaaacac ctaatgcaac
    11941 tgacgaagaa aagcaggctg ctgttaatca aatcaatcaa cttaaagatc aagcaattaa
    12001 tcaaattaat caaaaccaaa caaatgatca ggtagacaca actacaaatc aagcggtaaa
    12061 tgctatagat aatgttgaag ctgaagtagt aattaaacca aaggcaattg cagatattga
    12121 aaaagctgtt aaagaaaagc aacagcaaat tgataatagt cttgattcaa cagataatga
    12181 gaaagaagtt gcttcacaag cattagctaa agaaaaagaa aaagcacttg cagctattga
    12241 ccaagctcaa acgaatagtc aggtgaatca agcagcaaca aatggtgtat cagcgattaa
    12301 aattattcaa cctgaaacaa aagttaaacc agctgcacgt gaaaaaatca atcaaaaagc
    12361 gaatgaatta cgtgctaaga ttaatcagga taaagaagca acagcagaag aaagacaagt
    12421 agcactagat aaaatcaatg aatttgtaaa tcaagccatg acagatatta cgaataatag
    12481 aacaaatcaa caagttgatg atacaacaag tcaagcgctt gatagcattg ctttagtgac
    12541 gcctgaccat attgttagag cagctgctag agatgcagtt aagcaacaat atgaagctaa
    12601 aaagcgcgaa attgagcaag cggaacatgc gactgatgaa gaaaaacaag ttgctttaaa
    12661 tcaattagcg aataatgaaa aacgtgcatt acaaaacatc gatcaagcaa tagcgaataa
    12721 tgatgtgaaa cgtgttgaaa caaatggcat tgctacacta aaaggtgtac aacctcatat
    12781 tgtaattaag cctgaagcac aacaagcaat aaaagcaagt gcagaaaatc aagtagaatc
    12841 aataaaagat acaccacatg caacagttga tgaattagat gaagcgaatc aattaattag
    12901 cgacacactc aaacaagcgc aacaagaaat agaaaataca aatcaagatg ctgctgttac
    12961 tgatgttaga aatcaaacaa tcaaggcaat agagcaaata aaacctaaag taagacgtaa
    13021 acgagctgcg cttgatagca ttgaagaaaa taataaaaat caactcgatg caatccgaaa
    13081 tacgttggat actactcaag atgaaagaga tgttgctatt gatactttaa ataaaattgt
    13141 aaatacaatt aaaaatgaca ttgcacaaaa caaaacgaat gcagaagtgg atcgaactga
    13201 gactgatggc aacgacaaca tcaaagtgat tttacctaaa gttcaagtta aaccagcagc
    13261 gcgtcaatct gttggtgtaa aagccgaagc tcaaaatgca ctaatcgatc aaagcgattt
    13321 atcaactgaa gaagaaagac tagctgctaa acatttagta gaacaagcac ttaatcaggc
    13381 tattgatcag atcaatcatg cagataagac tgcccaagtt aatcaagata gtataaatgc
    13441 tcaaaatatt atttcaaaaa ttaaaccagc gacaacagtt aaagcaacag cattacaaca
    13501 aattcaaaat atcgctacaa ataaaattaa tttaattaaa gcaaataacg aagcgacaga
    13561 tgaagaacaa aatattgcaa tagcacaagt tgaaaaagag ttaattaaag ctaaacaaca
    13621 aattgctagt gcagtgacta atgcagatgt ggcatattta ttgcatgatg agaaaaacga
    13681 aattcgtgaa atcgaacctg ttattaacag aaaggcgtct gctcgagaac aattgacaac
    13741 attattcaac gataaaaaac aagcaattga agcgaatatt caagcaacgg tagaagaaag
    13801 aaatagtata ttagcacagt tacaaaatat ttatgacact gctattggac aaattgatca
    13861 agatcgtagc aatgcacaag ttgataaaac agcatcatta aatctacaaa caatacatga
    13921 tttagatgta catcctatta aaaagccaga tgctgaaaaa acgattaatg atgatcttgc
    13981 acgcgtcact gctttagtgc aaaattatcg aaaagtaagt aatcgtaata aggctgatgc
    14041 attaaaagct ataactgctt taaaattaca aatggatgaa gaattaaaaa cagcacgcac
    14101 taatgctgat gttgatgcag ttttaaaacg atttaatgtt gcattaagcg atatagaagc
    14161 agtaattact gaaaaagaaa atagcttact gcgaattgat aacattgctc aacaaacata
    14221 tgcgaaattc aaagcgatcg caacaccaga acaattagct aaagtaaaag tattaattga
    14281 tcaatatgtt gcagatggca atagaatgat tgatgaagat gcgacattaa atgacatcaa
    14341 acaacacacg caattcattg ttgatgaaat tttagcaatt aaattaccag ctgaagcgac
    14401 gaaagtatca ccaaaagaaa ttcagccagc tccaaaagtt tgtacgccta ttaaaaaaga
    14461 agagacacat gaatcgcgca aagttgaaaa agaacttcca aatacaggtt ctgaaggaat
    14521 ggatttacca ttgaaagaat ttgcactgat tacaggtgcg gctttgttag ctagaagacg
    14581 tactaaaaac gaaaaagaat cataattaac gaaatgacct taatataacg acacttttta
    14641 gtgactcagt tatattaagg tcatttttat ttgaatgaaa aagttgatct actaaagaga
    14701 atcgaaccaa attttatact attattccat tgatgcatgt gagtaatgtc cggcatataa
    14761 tccagcgaca tgtcctgtta cgagtgcact tgtaatatta taaccaccag tataaccatg
    14821 tatatctaat acttcaccac ataaaaataa tcccggaact aatttagaca tcattgtttt
    14881 aggttgaatt tctttaagtg acacaccacc acctgtgaca aatgccttat ctataggtaa
    14941 tgtcccgttc accttaaata caaacccttt aaacatattt actaagtcgt tcaattgttg
    15001 atttgataag tgatgtgatg tggtattttc atcgattcct gcttgttcca gcatgaacag
    15061 taagtagcgc tcttcaatta gaccatgcaa actgttttta atgattttat ctggtgtgtc
    15121 cgataataat gatgtgatgt gttgttttaa ttgttcatgg tttaattcag gaaatgcatc
    15181 gattgccata gaaatgtgct gtgtcttttg atttttttgt tctttataaa caaactgact
    15241 acatcttaat gcagctggac cactgatacc aaaatgagta aataacatat ccatttgatg
    15301 actgatgtgt tttttaccat ttttcttaag tactgacaat tcaacatctt ttaaacttaa
    15361 accttttaga cgattggatt tgatgaaagg ttcagctgat gtaattggaa cttcggtcgg
    15421 gaataactca gtaatggtat gacctaaatc ttgtgcgaac ttataaccat caccagttga
    15481 accagtttga gggacacttg taccacctgt agcaatcact agcgaatggc tttcataact
    15541 attattttga gtatgtacag tgaaagtttg gtctgtatta acttcgattc tactaacagc
    15601 ttcttcttct ttaatcgtta catgttggcg ttcgatagtt gtcactaatg tatcaaccac
    15661 gtcttgtgct ttgttggaaa ctggaaacat acgcccgtga tcttcttctt ttaatttaac
    15721 acccctagac tcaaaaaaat ctatgatgga ttcattatca aaaattgaaa agggactata
    15781 taaaaatttc ccatttccag gaatgttctt aataatttca gcatatggta atcgattagt
    15841 tacgttacat ctaccgccac cagatatttt gagtttacga cctagacctt tctttttttc
    15901 aatgagtaac acactgctac tttgttcgct tgcagctact gccgccatta agccgctagg
    15961 tccgcctccg ataataattg tttgatacat atacgcttcc tccaaatttg ttactggaca
    16021 ttcaattgct attttatagt acttttttaa actttgctat atctcttgtg ttatgagatg
    16081 acaattatgt tgaatatatt tataatgtga acttcataaa aaatgaagat gtatgtgtat
    16141 gataagaatg atttttctac tgttatgcga tgttcatctc aaatagtagt ataatgttta
    16201 tatgaaattt cgtaatattc taatcaaaat ctagtattca atgacaatgt gttgataatt
    16261 ttgttatgat aattcattga atatacgaca atgaaataaa gttttgtgat atattcataa
    16321 taatgaaaaa tgattttaca gagaaacagc ttttagttta caaaacttta ataaacatga
    16381 gatagggaga ttcgtaatga gtgaaagtaa agaaatggtg cgtggaacct ttttaattac
    16441 cattagtata ttaattacca aggtattggg cgtacttttt atcattccat tcaactattt
    16501 aattggtgga caagaaaata tggcgccgtt cacatacgct tatgcaccat ataatattgc
    16561 aattgcagtt gcgacagcag gtgtaccatt agcagcttcg aaatatgttg cgaaatataa
    16621 tgctattggg gcatataaag taagtcagaa attttataaa tcgagtttta ttgtaatgag
    16681 tattaccggt gttttaggtt tcttggtact gtatttctta gcaccttata tttctgaatt
    16741 aacattagct agaaacattc atgataaaaa cggttggtca gtcgatgata ttacttggat
    16801 tataagaatc attagtatgg ttgttatctt tattcctgta ttagcgacat ggagagggat
    16861 attccaaggt tataaatcta tggggccaac agcagtgtca gaagtaactg agcaaattgc
    16921 acgcgtgatt ttcatattaa tcggaagtta tttagtacta aatgtctttg atggctctat
    16981 tttattagcc aatggtattg ctacttttgc agcagcagtt ggtgcgatta ttggtatatt
    17041 cacactctgg tattactgga gaaagcgtaa gcataatatt gatcgtatgg tcgaatctga
    17101 ttacacagat atagatgtgt catatggcaa gatgtataaa gaaattattg cttatagtat
    17161 accgttcgtt attgtaagtt tgaattatcc attatttaac ttagtagatc aatttacaca
    17221 taacggtgct ttatctttag ttggtatacc ttcacagtta caagatatat tctttaatat
    17281 gctaaatatg tcaactaata aaattgtaat gattccgaca tcattaagtg ctggttttgc
    17341 agtgagttta attccttata ttacaaaaac atttgcagaa ggtcgcttac atgaaatgca
    17401 tcatcaaatt agaacatcta ttggtgtatt aatgtttata actgttccag ctagtattgg
    17461 aattatggcc ttggcacaac cattattcac agttttctat ggttacgatc caattgtttt
    17521 aggtcatgac cctaaccatg atggtagtcg cttattattc tattatgcgc ctgtagcaat
    17581 actaatttca ctattaagtg taacggcatc aatgttacaa ggtatagata aacagaaatt
    17641 aacagtttat gttattttag cttctgtagt tatcaagtta gcgttaaact atccattaat
    17701 tatgttattc catacacctg gtgcaatttt aagtactagt atcgcattat tatttgcaat
    17761 tggatgtaat ttctatattc ttaaaaaata tgcgaagttt aaattcagct atagttggat
    17821 tcattttgca aaaatattct tgtattcatt tattatgatg ctaggtgtag aattagtatt
    17881 tttccttgca aatctattct tagaacctac taaacttggt tacttaatca ttattatttt
    17941 aggtgttaca gtaggtattc taatttacgg tacaatcaca attaaaacgc gtcttgcgga
    18001 cgaattctta ggtgaaattc ctgaaaaatt aagacgtaga gttaggtttt tacgatgaga
    18061 atagataaat ttttagcaaa tatgggcgtc ggtacacgta atgaggtcaa acagttactt
    18121 aaaaaaggcc ttgtaaatgt caacgaacaa gtaattaaat caccaaaaac acatatagaa
    18181 ccagaaaacg ataagataac agttcgtgga gaattaatag aatacattga aaatgtctat
    18241 attatgttaa ataagccaaa aggttatata tcagcaactg aagatcatca ttcaaaaacc
    18301 gttattgatt taatacctga ataccaacat ctaaatattt ttccggttgg tcgtcttgat
    18361 aaagatacag aagggctttt attgataacg aatgatggtg attttaatca tgaattaatg
    18421 agtccaaaca aacatgtttc taaaaaatat gaagttattt cagcaaatcc tatcactgaa
    18481 gacgacattc aagcttttaa agagggtgta acactgactg atggcaaggt taagcctgca
    18541 atattaactt atattgataa tcaaacgtca cacgtaacaa tttatgaagg aaaatatcat
    18601 caagttaaac gaatgtttca tagcattcaa aatgaggtat tacatttaag acgtatcaaa
    18661 attgctgatt tagaattgga cagcaattta gattcaggag aatatcgttt attaactgaa
    18721 aatgattttg acaaattaaa ttataaataa aagggagacg attgatatgg caaaaggtaa
    18781 tttatttaaa gcgattttag gtataggtgg cgctgtagca gctgtacttg ttacacgtaa
    18841 agatagtcgt gacaagctga aagcagaata taataaatat aaacaagatc ctcaaagcta
    18901 taaagataat gctaaggata aagcgacgca attaggaaca attgctaatg aaacaattaa
    18961 agaagtaaaa acaaatccga aagaatatgc taatagatta aaaaataatc caaaagcatt
    19021 tttcaaagaa gaaaaatcaa aatttaccga atatgacaat aagactgacg aaagtattga
    19081 aaaaggtaaa tttgatgatg aaggtggcgc agcaccaaat aataatttac gtatcgtcac
    19141 tgaagaagat ttaaaaaaga ataaaaatgc attatctgat aaagaataag atgtgagtag
    19201 gacagaaatg ataagggagt gggacagaaa tcatatttta gcaaaattta tttcgtcgtc
    19261 ccaccccggc aaggttgact agaattgaaa aaagcttgtt acaagcgtat tttcattcag
    19321 tcaactactg ccaatataat attgtaaact ataggacatt tattagtgtt tcaggttctt
    19381 tatcgttagt catagctaat atgcatatag aaagaagaat acatgcgtga aactcattca
    19441 taagaaatac taatatctaa agaaaacgta tttctttatc ttttcgaaat tctctgtgtt
    19501 ggggccctta ccccaacttg cacattattg aaagctgact attggccagc ttctgtgttg
    19561 gggcccttac cccaacttgc acattattga aagctaacta ttggccagct tctatgttgg
    19621 ggccccgcca acttgcattg tctgtagaat ttcttttcga aattctctat gttggggccc
    19681 cggggcgcat tttcgttcgg ttaattactg tcaatattaa attgtgtgcc tagtacattg
    19741 acttatatct cgagctcttg aatatgtgta ttttaaatat ataaattaaa aaaggaacaa
    19801 agcagctatt tatgatttgt gtcatatcaa tagcatcgtt ttgttccttt ttattatttt
    19861 ctgacatctt attgtagttt ttatatcttt tcatgtaaaa tagaatttaa cttatagaaa
    19921 aaaggaagtc gatttcgaat gtggaaagaa aaagttcaac aatacgaaga tcaaatcatt
    19981 aatgacttaa aaggattatt agcaattgaa agtgtgagag atgatgcaaa agcatcagaa
    20041 gacgcaccag ttggtccagg tcctcgtaag gcattagact acatgtatga aattgcacat
    20101 agagatggat ttacaacaca tgatgtggat catattgcag gaagaattga ggcaggtaaa
    20161 ggaaatgacg tattaggtat cttatgtcat gttgacgttg ttcctgctgg tgatggatgg
    20221 gatagtaatc cgttcgagcc ggttgtaaca gaagatgcta tcatagctag aggtacactt
    20281 gatgacaaag gtccaacaat tgctgcttat tatgcaatta agatattaga agatatgaat
    20341 gtggattgga agaaacgtat tcatatgatt attggtacgg atgaagaatc tgattggaaa
    20401 tgtacggatc gctattttaa aacagaagaa atgccaacat taggttttgc accagatgca
    20461 gaatttccat gtattcatgg tgaaaaaggc attacaacat ttgatttagt tcaaaataaa
    20521 cttactgaag atcaagatga acctgattat gaattaataa cttttaaatc tggtgaacgt
    20581 tacaacatgg tacctgatca tgcagaagca agagtgcttg ttaaagaaaa tatgacagat
    20641 gttattcaag actttgagta ctttttagaa caaaatcatt tacaaggtga tagtactgtt
    20701 gatagtggca ttctagtttt aacagttgaa ggtaaagcgg ttcatggtat ggatccatct
    20761 atcggtgtga atgcgggtct ttacttacta aaattcttag catcattaaa tcttgataat
    20821 aatgcacaag cgtttgtagc atttagtaat cgctacttat ttaattcaga ttttggtgaa
    20881 aagatgggaa tgaaattcca tacagatgtc atgggtgacg tgacaactaa cattggtgtt
    20941 attacatatg ataatgaaaa cgcaggtctt ttcggtatca acttacgcta cccagaagga
    21001 tttgaatttg aaaaagctat ggatcgtttt gcaaatgaga ttcaacaata tggctttgaa
    21061 gtgaaattag gtaaagtcca accaccacat tatgttgata aaaatgatcc ttttgtacaa
    21121 aagttagtta ctgcatatag aaatcaaaca aatgatatga ctgaacctta tactataggt
    21181 ggcggtactt atgcgagaaa cttagacaag ggtgtagcat ttggcgcaat gtttagtgat
    21241 tctgaagatt taatgcatca gaaaaatgaa tatatcacta aaaaacagtt atttaacgca
    21301 actagtattt acttagaagc aatttattca ttatgcgtgg aggaataata tatggaaaaa
    21361 atttttttaa atggtgagtt tgtaagtcca agtgaagcaa aggtttcata caacgacaga
    21421 ggatacgtat ttggcgatgg tatttatgaa tacattcgag tatataatgg taagttattt
    21481 acagtaacag aacattatga aagattttta cgtagtgcca atgagattgg tttagattta
    21541 aattattctg tagaagaatt aattgaacta tctcgtaaat tagttgatat gaatcaaatt
    21601 gaaactgggg caatttatat tcaagcaacg cgtggtgtag ctgaaaggaa tcatagcttc
    21661 ccgacacctg aagtagaacc agcaattgtt gcttatacaa agagttatga tcgtccttat
    21721 gatcatttag aaaatggtgt gaatggtgtt accgttgaag atatccgatg gttacgttgc
    21781 gacattaaaa gcttgaactt attaggaaat gtattagcaa aagaatatgc tgtgaaatat
    21841 aatgcagttg aagcaattca acatcgaggt gaaactgtaa ctgaaggatc ttcaagtaat
    21901 gcttatgcaa ttaaagacgg tgtgatttat acacatccga ttaacaacta tattcttaat
    21961 ggtattacac gaattgtaat taaaaaaatt gccgaagact ataacatccc atttaaagaa
    22021 gaaacgttta ctgtagattt cttgaaaaac gcagatgaag ttattgtttc aagtacttca
    22081 gctgaggtta cacctgttat taaattagat ggtgaaccag ttaatgatgg taaagttggc
    22141 ccaattacac gtcaactaca agaaggattt gaaaagtata tagagtcaca cagtatttaa
    22201 aattctttca tcatattttt agattaaatt acttaaattt atcaactttc gtaagtgaat
    22261 tatgttataa tttaaaacga agcttaaaag tgttgtttag aaaacacata ttagtaaaaa
    22321 gtgccatttt gtttctcatt ttcagttgaa atataatgat aaaaattata aaattttcta
    22381 tgagttacaa atatgatgtg catcatattt tactaagtac gtaaatgtta cagttcataa
    22441 atttatgcta accatgaaaa tgggatagca attattttga aaactagagt ttatcgccta
    22501 cagagattca aaaacatgta gttttagaca cggtaacatt atatgtcaag taagtgacta
    22561 tagaatgtat ggtgtcaatg acagacatta cgatttaaat ttagtcgtgt aatgtaggca
    22621 ttggttgagg atttacaata attatctgga cataatattt gtaccagtat ggtgttataa
    22681 attagaatat atggctcttg aaaaacctat ttctagagcc attttctaat tgaaagtgag
    22741 gtggacgagt tggagcagtt ttatcaatta gggtggacac ttgattcagc aggtggtgca
    22801 tctggtgaag catatatggc tgaacaagat ggacaaaagt tgtttttaaa acgaaattca
    22861 aatccattta ttgcggcatt atcagcagaa ggtattgtgc ccaaattagt atggacgaaa
    22921 cgcatagaaa caggcgaggt tgttacagca caacattgga aaaatgggcg tgaactatct
    22981 tcaaacgaaa tgaagcaaac aagagttgca catttattaa agaagataca caattctaga
    23041 cctttattaa gtatgttaaa gcgtatggaa atggaaccta ttactcctga gattatgctt
    23101 aataaaatta atgcctcttt atcaagagaa gttttaacac atcatattgt gagaaaatca
    23161 ttaacctatt tagaagagca tataccgagt ttagattcgc gttttttcac tgttgtacat
    23221 ggtgatgtga atcataataa ttggttatta tccgatcgag atgaactatt tttagttgat
    23281 tgggaaggtg caatgattgc agacccggca attgatatcg gaatgctgct ttacaactat
    23341 gttccacaac aacaatggtc tgaatggctt gaaacatatg gtgttcaaga gagcttgaat
    23401 ttaaataaac gtatgaaatg gtatacggta attcaatcta taggtcttgt tcaatggtat
    23461 gaagagcaaa aaagatataa ggatatgaac acttggctta aattcctaaa tgaagttatg
    23521 aatagtaaca tgtttattta agaggagaat aaaatatgag agttcgatac aaaccatggg
    23581 ctgaagatta tttaaaagat catcctgaat tggttgacat ggacggtcaa catgcaggga
    23641 aaatgactga atggtttgat aaaacgcaac cgatacacat tgaaattggt tcgggtatgg
    23701 ggcagtttat tacaacatta gctgctcaaa atcctcatat taactatatt tcaatggaac
    23761 gtgagaaaag tatcgtttat aaagtattgg ataaagttaa agagatgggt ttaacaaatt
    23821 taaagattat ttgtaatgac gccattgaat taaatgaata ttttaaagac ggcgaagttt
    23881 cgcgtattta tttgaacttt tcagatccat ggccaaaaaa tcgtcatgca aaacgacgtt
    23941 taacgtatca tacgttttta gcgttgtatc aacaaatctt aaatgatgaa ggagatttac
    24001 attttaaaac tgataacaga ggactatttg cctacagttt agaaagtatg tctcaatttg
    24061 gaatgtattt tacaaaaata aatctaaatt tacatcaaga agacgatggc agtaatattt
    24121 taacagaata tgaaaagaaa ttttcggata aagggtcacg tatttatcgt atggaagcga
    24181 aattccattc acaaaaatag agcacttatg agggtatgta cacatacaat cataagtgtt
    24241 ttttcgtact ataatggttc tatagataaa ggttctataa taaatcggac tgatggaaaa
    24301 agtttttaac ttttcatctg tccgattttt tgattttgaa tataaaaaaa gcgcaattat
    24361 ctctataatt aaaagtgact aaacaaataa ctaaaggaga taatgcgcct atgaacaatg
    24421 ttatactaaa tacctttgat ttaaaagaat aaagcttgca ttttacttgt aaattttaat
    24481 tattaattta aaaatacagg ccgatggtta tgatgcattt acatataata tgattgcatt
    24541 aatctttata agtagttaga agtgtattag tgtggggcgt ttcgtttttg ttcgcattta
    24601 tgaatagtga tatgatgtaa taaacacatg ctaattgtaa taacgaatac agaaagaata
    24661 ggtgatgtta tgaaaatcgg ggatatatct attcattatc taaatggtgg caatacaaaa
    24721 atggatggcg gtgcaatgtt tggtgttgtt ccgaagccgt tgtggtcaaa gcaatacaat
    24781 gcaaatgaac gaaatcaaat caatttaccg acacatccaa ttttgattca aacggctcaa
    24841 tataatttga ttatagatgc aggtattggt aatggtaaat tatctgaaaa gcaattacgt
    24901 aattttggag tagatgaaga aagtcatata attgctgatt tggcaaatta taatttaacg
    24961 ccaaaggata ttgattatgt gctaatgaca catatgcatt ttgatcatgc tgccggtttg
    25021 actgatcaag caggacatgc aatttttgaa aatgcgattc atgttgtgca acaagatgag
    25081 tggcatgagt ttattgcacc taatataaga agtaaatcaa cttactggga taagaataaa
    25141 ggcgattata gtaacaagtt gattttattc gaaaaacatt ttgaaccggt tctgggtatc
    25201 aagatgcaac atagtggagg tcatagcttt ggccacacga ttattacgat tgaaagtcaa
    25261 ggagataaag cagttcatat gggtgatata ttcccaacta ctgcacataa aaatcctcta
    25321 tgggtaacgg catatgatga ttatcctatg caatcgattc gtgaaaaaga acgcatgata
    25381 ccatatttta ttcagcaaca atattggttc ttgttttatc atgatgaaaa ctactttgct
    25441 gtaaaataca gcgataatgg tgaaaacata gatgcatata ttttacgtga aacattagtt
    25501 gataataact aaaataaaga tgtattacta aacaaatttt caaaaataaa aaattgagcc
    25561 acatccaatc ttactaatta gggtgtggct catttttaag ttttacgatc caaatcaaat
    25621 atggataaaa ttcgtattaa cgctctacaa tgttaatgac ttcaccagta tatgcatctg
    25681 cataaaaatc ataatgaata ttttgaccat ttttaatagt tgtaattcca ccttgataaa
    25741 ctaaacggta tttatcagtt tcaggatgaa cgaatggttc ataaacgata tatgagcctt
    25801 tgacattcat aaaatatgat tttacttcat taagtacttt aacgggatta tagcgtttac
    25861 gattaataaa ttggatgatt gaaatggtag aaataattac aatggctact gcaattattg
    25921 ttggaattat atatttcagt ttagtcatca tttatgctcc cttgatttca aattcatatc
    25981 attagtttac catattgaag atgatataat aacttataag gagtgagaac ttttatgaac
    26041 ataaataaaa aagtaacatt acaacgaatt caaactttaa ctgagcttca tggggcacca
    26101 ggttttgaag aagaagtaaa aaattatatg actcagcaaa tggcgccgta cgtagatgaa
    26161 tttattgaaa atcgtatggg tggatttttt ggtgtgaaaa aatctaaaaa tccaaatgca
    26221 aaacgtgtaa tgattgcagc acatatggat gaaatcggat ttatgattac aaatatcact
    26281 aaaaatggaa tgattcaatt cacaaattta ggtggtgttg caaatgatat ttggcaagga
    26341 caacgcttag taattaaaaa tagaaatggc gataaaatta tcggtgttgt ttctaatata
    26401 cctaaacatt ttcgtactgg tagtgaaggt gcaccggaaa ttaaagattt aacattagat
    26461 ataggtgctc aaaatgaaga tgaggtgcgt gagcgcggaa tagatatagg agatacaatt
    26521 gtacctcaca cgccattcac acagttatct gaacatcgat atagtgctaa agcatgggat
    26581 aatcgttatg gttgtgtctt ggcaattgaa atactagaat tattaaaaga tatagaatta
    26641 gatgtagact tgtatgttgg cgcaaatgtt caagaagagg ttggattacg aggtgcgaaa
    26701 gcatctgcag agatgataga cccagacgtt gcatttgtag ttgattgttc acctgccaat
    26761 gacgttaaag gaaaccaacc attatctggt gaacttggta aagggacgtt aattcgcata
    26821 aaagacggta caatgatttt aaagcctgta tttagagact atttattaaa gttagtagaa
    26881 gcacatgaca ttgaacatca atactatatg tcaccaggtg gaacagatgg tggagaaatt
    26941 cataaagcta atattggtat tccgactgca gttattggtg tatgtgcacg atatattcat
    27001 agtacagact cagtatttga tataagagac tattttgcag ctagatcttt actttcagaa
    27061 gccatttgta atttagataa taatcaaata gaaacattac aatataaata atcgggtaat
    27121 aacaactatt atctctaaat agttatatat aatcattaat taaggagaca taaaaatgaa
    27181 acaacttgaa tcagaacaac aatttgaatc tttaaaacaa ggtgctacag tatttgaatt
    27241 cactgcaggc tggtgtccag attgtagagt gatagaacca gatttaccgg aattagaagc
    27301 gagatatcct atgtttgact tcgtatcagt agaccgtgat aaatttatgg atatttgtat
    27361 tgaaaatggt attatgggta ttccaagttt tctagtatat aaaaatggag aactgcttgg
    27421 aagttatatt ggaaaagaac gaaaatcaat tgaacagata gatgcatttt tagctcaata
    27481 cgtgtaattt agactagaga aaaacggggt aatactcgtt tttctctgtt actatgtgtt
    27541 gatttattgt aaactattat aaggtgcgaa attaggagtg ttacatatga atacctttca
    27601 aatgagagat aaattaaagg aacgtttaag ccatttagac gttgatttta aatttaatcg
    27661 tgaagaagaa actttgcgta tttatcgaac agataataac aaaggtatca cgattaaact
    27721 taacgctata gtcgcaaaat atgaagataa aaaagaaaaa attgtagatg aaattgttta
    27781 ttacgttgat gaagctattg cacaaatggc agataaaacc cttgagagta tatcatctag
    27841 tcaaattatg cctgtcatta gagcgactag ctttgataaa aaaactaaac aaggtgttcc
    27901 ttttatctat gatgagcata ctgcagaaac agcagtttat tatgcagtcg atttagggaa
    27961 atcatatcgt ctaattgacg aaagcatgtt agaggatttg aagttaactg aacaacaaat
    28021 aagagaaatg tctctgttta atgttagaaa attgtcaaat tcatatacga ctgatgaagt
    28081 aaaaggtaat attttttatt ttattaactc aaatgacggg tatgatgcaa gtaggatact
    28141 aaatactgca tttttaaatg aaattgaggc acaatgtcaa ggcgaaatgc tcgtagcagt
    28201 gccacaccaa gatgtgttaa ttattgcaga tatacgcaat aaaacaggat atgatgtgat
    28261 ggcacattta acaatggaat ttttcactaa aggtctagtt ccaattacat cattatcctt
    28321 tggatataaa cagggtcatc ttgaaccgat atttatttta ggtaaaaata ataaacaaaa
    28381 aagagatcca aacgtgattc agcgtttaga agcaaatcgt cgtaaattta ataaagataa
    28441 atagaaataa ttggataagg agttttgtca taatgaattt attttacaat cctaaatatg
    28501 taggagatgt cgcattttta caaattgaac cagttgaagg tgaattaaac tacaataaaa
    28561 aaggtaatgt tgttgaaatt actaatgaag gtaatgttgt aggttataat atttttgaaa
    28621 tttcaaaaga tataacaatt gaagaaaaag gtcatattaa attaactgat gaacttgtaa
    28681 atgtattcca aaagcgtatt tcagaagctg gttttgatta taaattaaat gctgatctat
    28741 caccgaaatt tgtagttggc tacgttgaaa ctaaagacaa acatcctgat gcagataaat
    28801 taagtgtact aaatgtaaac gttggaaatg acacattaca aattgtatgt ggcgcgccta
    28861 acgttgaagc tggacagaaa gttgttgttg ctaaagtagg tgcagtgatg cctagcggta
    28921 tggtaattaa agatgctgaa ttacgtggtg ttgcctcaag cggtatgatt tgttcaatga
    28981 aagaattgaa tttacctaat gcacctgaag aaaaaggtat tatggtatta aatgacagct
    29041 atgaaattgg acaagcattt tttgaataat taaggaaggt agtgaaaata tgagctggtt
    29101 tgataaatta ttcggcgaag ataatgattc aaatgatgac ttgattcata gaaagaaaaa
    29161 aagacgtcaa gaatcacaaa atatagataa cgatcatgac tcattactgc ctcaaaataa
    29221 tgatatttat agtcgtccga ggggaaaatt ccgttttcct atgagcgtag cttatgaaaa
    29281 tgaaaatgtt gaacaatctg cagatactat ttcagatgaa aaagaacaat accatcgaga
    29341 ctatcgcaaa caaagccacg attctcgttc acaaaaacga catcgccgta gaagaaatca
    29401 aacaactgaa gaacaaaatt atagtgaaaa acgtgggaat tctaaaatat cacagcaaag
    29461 tataaaatat aaagatcatt cacattacca tacgaataag ccaggtacat atgtttctgc
    29521 aattaatggt attgagaagg aaacgcacaa gccaaaaaca cataatatgt attctaataa
    29581 tacaaatcat cgtgctaaag attcaactcc agattatcac aaagaaagtt tcaagacttc
    29641 agaggtaccg tcagctattt ttggcacaat gaaacctaaa aagttagaaa atggtcgtat
    29701 ccctgtaagt aaaccttcag aaaaagttga gtcagataaa caaaaatatg ataaatatgt
    29761 agctaagacg caaacgtctc aaaataaaca attagaacaa gaaaaacaaa atgatagtgt
    29821 tgtcaaacaa ggaactgcat ctaaatcatc tgatgaaaat gtatcatcaa caacaaaatc
    29881 aatgcctaat tattcaaaag ttgataatac tatcaaaatt gaaaatattt atgcttcaca
    29941 aattgttgaa gaaattagac gtgaacgaga acgtaaagtg cttcaaaagc gtcgatttaa
    30001 aaaagcgttg caacaaaagc gtgaagaaca taaaaacgaa gagcaagatg caatacaacg
    30061 tgcaattgat gaaatgtatg ctaaacaagc ggaacgctat gttggtgata gttcattaaa
    30121 tgatgatagt gacttaacag ataatagtac agatgctagt cagcttcata caaatggcat
    30181 agagaatgaa actgtatcaa atgatgaaaa taaacaagcg tcaatacaaa atgaagacac
    30241 taatgacact catgtagatg aaagtccata caattatgag gaagttagtt tgaatcaagt
    30301 atcgacaaca aaacaattgt cagatgatga agttacggtt tcgaatgtaa cgtctcaaca
    30361 tcaatcagca ctacaacata acgttgaagt aaatgataaa gatgaactaa aaaatcaatc
    30421 cagattaatt gctgattcag aagaagatgg agcaacgaat aaagaagaat attcaggaag
    30481 tcaaatcgat gatgcagaat tttatgaatt aaatgataca gaagtagatg aggatactac
    30541 ttcaaatatc gaagataata ccaatagaaa cgcgtctgaa atgcatgtag acgctcctaa
    30601 aacgcaagag tacgcagtaa ctgaatctca agtaaataat atcgataaaa cggttgataa
    30661 tgaaattgaa ttagcaccgc gtcataaaaa agatgaccaa acaaacttaa gtgtcaactc
    30721 attgaaaacg aatgatgtga atgataatca tgttgtggaa gattcaagca tgaatgaaat
    30781 agaaaagaat aacgcagaaa ttacagaaaa tgtgcaaaac gaagcagctg aaagtgaaca
    30841 aaatgtcgaa gagaaaacta ttgaaaacgt aaatccaaag aaacagactg aaaaggtttc
    30901 aactttaagt aaaagaccat ttaatgttgt catgacgcca tctgataaaa agcgtatgat
    30961 ggatcgtaaa aagcattcaa aagtcaatgt gcctgaatta aagcctgtac aaagtaagca
    31021 agctgtgagt gaaagaatgc ctgcgagtca agccacacca tcatcaagat ctgattcaca
    31081 agagtcaaat acaaatgcat ataaaacaaa taatatgaca tcaaacaatg ttgagaacaa
    31141 tcaacttatt ggtcatgcag aaacagaaaa tgattatcaa aatgcacaac aatattcaga
    31201 gcagaaacct tctgttgatt caactcaaac ggaaatattt gaagaaagtc aagatgataa
    31261 tcaattggaa aatgagcaag ttgatcaatc aacttcgtct tcagtttcag aagtaagcga
    31321 cataactgaa gaaagcgaag aaacaacaca tccaaacaat actagtggac aacaagataa
    31381 tgatgatcaa caaaaagatt tacagtcatc attttcaaat aaaaatgaag atacagctaa
    31441 tgaaaataga cctcggacga accaacaaga tgttgcaaca aatcaagctg tacaaacatc
    31501 taagccgatg attcgtaaag gcccaaatat taaattgcca agtgtttcat tactagaaga
    31561 accacaagtt attgagtcgg acgaggactg gattacagat aaaaagaaag aactgaatga
    31621 cgcattattt tactttaatg tacctgcaga agtacaagat gtaactgaag gtccaagtgt
    31681 tacaagattt gaattatcag ttgaaaaagg tgttaaagtt tcaagaatta cggcattaca
    31741 agatgacatt aaaatggcat tggcagcgaa agatattcgt atagaagcgc ctattccagg
    31801 aactagtcgt gttggtattg aagttccgaa ccaaaatcca acgacagtca acttacgttc
    31861 tattattgaa tctccaagtt ttaaaaatgc tgaatctaaa ttaacagttg cgatggggta
    31921 tagaattaat aatgaaccat tacttatgga tattgctaaa acgccacacg cactaattgc
    31981 aggtgcaact ggatcaggga aatcagtttg tatcaatagt attttgatgt ctttactata
    32041 taaaaatcat cctgaggaat taagattatt acttatcgat ccaaaaatgg ttgaattagc
    32101 tccttataat ggtttgccac atttagttgc accggtaatt acagatgtca aagcagctac
    32161 acagagttta aaatgggccg tagaagaaat ggaacgacgt tataagttat ttgcacatta
    32221 ccatgtacgt aatataacag catttaacaa aaaagcacca tatgatgaaa gaatgccaaa
    32281 aattgtcatt gtaattgatg agttggctga tttaatgatg atggctccgc aagaagttga
    32341 acagtctatt gctagaattg ctcaaaaagc gagagcatgt ggtattcata tgttagtagc
    32401 tacgcaaaga ccatctgtca atgtaattac aggtttaatt aaagccaaca taccaacaag
    32461 aattgcattt atggtatcat caagtgtaga ttcgagaacg atattagaca gtggtggagc
    32521 agaacgcttg ttaggatatg gcgatatgtt atatcttggt agcggtatga ataaaccgat
    32581 tagagttcaa ggtacatttg tttctgatga cgaaattgat gatgttgttg attttatcaa
    32641 acaacaaaga gaaccggact atctatttga agaaaaagaa ttgttgaaaa aaacacaaac
    32701 acaatcacaa gatgaattat ttgatgatgt ttgtgcattt atggttaatg aaggacatat
    32761 ttcaacatca ttaatccaaa gacatttcca aattggctat aatagagcag caagaattat
    32821 cgatcaatta gagcaactcg gttatgtttc gagtgctaat ggttcaaaac caagggatgt
    32881 ttatgttacg gaagcagatt taaataaaga ataattatga gtaaggagtt ttatataatg
    32941 acacactatc attttgtcgg aattaaaggt tctggcatga gttcattagc acaaatcatg
    33001 catgatttag gacatgaagt tcaaggatcg gatattgaga actacgtatt tacagaagtt
    33061 gctcttagaa ataaggggat aaaaatatta ccatttgatg ctaataacat aaaagaagat
    33121 atggtagtta tacaaggtaa tgcattcgcg agtagccatg aagaaatagt acgtgcacat
    33181 caattgaaat tagatgttgt aagttataat gattttttag gacagattat tgatcaatat
    33241 acttcagtag ctgtaactgg tgcacatggt aaaacttcta caacaggttt attatcacat
    33301 gttatgaatg gtgataaaaa gacttcattt ttaattggtg atggcacagg tatgggattg
    33361 cctgaaagtg attatttcgc ttttgaggca tgtgaatata gacgtcactt tttaagttat
    33421 aaacctgatt acgcaattat gacaaatatt gatttcgatc atcctgatta ttttaaagat
    33481 attaatgatg tttttgatgc attccaagaa atggcacata atgttaaaaa aggtattatt
    33541 gcttggggtg atgatgaaca tctacgtaaa attgaagcag atgttccaat ttattattat
    33601 ggatttaaag attcggatga catttatgct caaaatattc aaattacgga taaaggtact
    33661 gcttttgatg tgtatgtgga tggtgagttt tatgatcact tcctgtctcc acaatatggt
    33721 gaccatacag ttttaaatgc attagctgta attgcgatta gttatttaga gaagctagat
    33781 gttacaaata ttaaagaagc attagaaacg tttggtggtg ttaaacgtcg tttcaatgaa
    33841 actacaattg caaatcaagt tattgtagat gattatgcac accatccaag agaaattagt
    33901 gctacaattg aaacagcacg aaagaaatat ccacataaag aagttgttgc agtatttcaa
    33961 ccacacactt tctctagaac acaggcattt ttaaatgaat ttgcagaaag tttaagtaaa
    34021 gcagatcgtg tattcttatg tgaaattttt ggatcaatta gagaaaatac tggcgcatta
    34081 acgatacaag atttaattga taaaattgaa ggtgcatcgt taattaatga agattctatt
    34141 aatgtattag aacaatttga taatgctgtt attttattta tgggtgcagg tgatattcaa
    34201 aaattacaaa atgcatattt agataaatta ggcatgaaaa atgcgtttta atatgtttat
    34261 aatagagtag tatgggtatt tattattaat gacattatta catgttaatt aggaggcgtt
    34321 tttaatggat tggattttac caattgctgg aattatcgct gcgattgcat tcttaatttt
    34381 atgtatcggt atcgtagctg tattaaattc tgttaagaaa aacttagatt atgttgcaaa
    34441 aacacttgac ggtgtagaag gtcaagttca aggtattact cgtgaaacaa cagatttact
    34501 tcataaagta aaccgtttaa ctgaggatat ccaaggtaaa gtagatcgtt taaactcagt
    34561 tgtagatgct gttaaaggta tcggtgactc agtacaaacg ttaaacagct ctgtagatcg
    34621 tgtaacaaat tcaattacac ataatatttc tcaaaatgaa gataaaatct cacaagttgt
    34681 tcaatggtca aatgttgcaa tggaaattgc agacaaatgg caaaatagac actaccgtcg
    34741 tggaagtgca aattacaaag ctaataatgt agcaactgat gcaaatcata gctatacttc
    34801 tagagtagat aaataattaa taattaaaca aatgcactta aatgaggttg ttacttattt
    34861 aagtgcattt gttataggag gatatacaaa tgacgcagca acaaaatgat aaaagaacat
    34921 taaaaaataa acacacttat caaaatgaac cactaccaaa ccgtaaagat tttgttgtta
    34981 gttttataac tggcgcgctt gttggttcag ctttaggttt gtattttaaa aataaagttt
    35041 atcaaaaagc agatgattta aaagtcaaag aacaagaact gtcgcaaaag tttgaagaaa
    35101 gaaaaacgca acttgaagaa acggttgcct atacaaaaga acgtgttgaa ggatttttaa
    35161 acaaatctaa aaatgagcaa gcggcattaa aggcacaaca agcagcgata aaagaagaag
    35221 caagtgcaaa taatttaagt gatacatcac aagaagcaca agagattcaa gaagctaaaa
    35281 gagaagcaca agcagaagcg gataaaagtg tggctgtatc aaataaagaa tcaaaagcag
    35341 tggcattgaa agcacaacaa gcagcgataa aagaagaagc aagtgcaaat aatttgagtg
    35401 atacatcaca agaggcacaa gagattcaag aagctaaaaa agaagcacaa gcagaaacag
    35461 ataaaagtgc agctgtatca aatgaagaac caaaagcagt ggcattgaaa gcacaacaag
    35521 cagcgataaa agaagaagca agtgcaaata atttaagtga tatatcacaa gaggcacaag
    35581 aggttcaaga agctaaaaaa gaagcacaag cagagaaaga cagtgacaca ttaactaaag
    35641 atgcaagtgc agcaaaggta gaagtatcaa aaccagagtc acaagctgaa agattagcaa
    35701 acgctgcaaa acagaagcaa gctaaattaa caccaggttc aaaagagagt caattaactg
    35761 aagcgttatt tgcagaaaaa ccagttgcta aaaatgactt gaaagaaatt cctcaattag
    35821 ttactaaaaa gaatgatgta tcagagacag agacggttaa tatagataat aaagacactg
    35881 ttaaacaaaa agaagctaaa tttgaaaatg gtgttattac acgtaaagct gatgaaaaaa
    35941 caactaataa tacagctgtt gacaagaaat caggtaaaca atctaaaaaa acaacacctt
    36001 caaataaacg aaatgcatca aaagcatcta caaataaaac ttcaggtcag aaaaagcaac
    36061 ataataagaa atcatcacaa ggtgcaaaga aacaaagtag ttcaagtaag tcaactcaaa
    36121 agaataatca aactagtaat aagaattcaa aaacaacaaa tgctaaatca tccaatgcat
    36181 caaaaacgcc aaatgctaaa gttgagaaag ctaaaagtaa aatagagaaa cgtacattca
    36241 atgactaaga ttactaaaat cgttgaataa tcaatcatag cagtaagcta tgtaatatat
    36301 ttttaaaaat tgaatttcaa aataaaaggt aggcattcag gtgcttacct tttttgatat
    36361 catcagttta ttttgaaagg caatgcgatc atttcatgta tttatgttgt ttgaaacatc
    36421 gttagataac aatagtgata ttgcacattt taagctgaag atggaaaaat attcgataat
    36481 caaataaaca atgaatttta gaaggtacaa tgacgttcac taatttaaat atagctgaat
    36541 gtgttggtga gtgatgttca ctatagattt atattaatat acaaagacaa aggttgttaa
    36601 tttttattaa gcgttaggtt gaatgtatga gaattttaga tttataatag aagatagaaa
    36661 cgaaaatttt tcttaaaagc agtaatgttg actcaaaata agctataata atgacactta
    36721 tttaattgat taacatttgc taataaatat caatatagaa tataactttc caataatgac
    36781 tgagaaaatc gaaatgtcag tctcgaatca tataattaga aaattgatta ttttctgtca
    36841 atttagg
//
LOCUS       DALAGD010000013        36730 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.013, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000013 DALAGD010000000
VERSION     DALAGD010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 36730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36730
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.013"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(247..813)
                     /locus_tag="O2701_000472"
     CDS             complement(247..813)
                     /locus_tag="O2701_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCZ4292382.1"
                     /translation="MKGLIIIGSAQVNSHTSALARYLTEHFKTHDIEAEIFDLAEKPL
                     NQLDFSGTTPSIDEIKQNMKDLKEKAMAADFLILGTPNYHGSYSGILKNALDHLNMDY
                     FKMKPVGLIGNSGGIVSSEPLSHLRVIVRSLLGIAVPTQIATHDSDFAKNEDGSYYLN
                     DSEFQLRARLFVDQIVSFVNNSPYEHLK"
     gene            complement(834..1703)
                     /locus_tag="O2701_000473"
     CDS             complement(834..1703)
                     /locus_tag="O2701_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCZ4292383.1"
                     /translation="MIKLIATDMDGTLLNAAHEISQPNIDAIKYAQEQGITVVIATGR
                     AFYEAQAPVADTDLTVPYICLNGAEVRDETFNVMSTSHLNKSLVHKITNVLKDAGIYY
                     QVYTSRAIYTEDPQRDLDIYIDIAERAGQHANVERIKNGIQRRIDNGTLKVVDNYDAI
                     ENIPGELIMKILAFDGNLEKIDKASKILAESPNLAISSSSRGNIEITHSDAQKGIALE
                     TIAERLGIEMKEVMSIGDNLNDLSMLEKVGYPVAMENGAEEVKKIAKYVTDTNENSGV
                     GKAIMKLLREQQV"
     gene            complement(1850..2320)
                     /locus_tag="O2701_000474"
     CDS             complement(1850..2320)
                     /locus_tag="O2701_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HCZ4292384.1"
                     /translation="MTNDIYFMTLAIEEAKKAAQLGEVPIGAIITKDDEVIARAHNLR
                     ETLQQPTAHAEHIAIERAAKVLGSWRLEGCTLYVTLEPCVMCAGTIVMSRIPRVVYGA
                     DDPKGGCSGSLMNLLQQSNFNHRAIVDKGVLKEACSTLLTTFFKNLRANKKSTN"
     gene            2387..3004
                     /locus_tag="O2701_000475"
     CDS             2387..3004
                     /locus_tag="O2701_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCZ4292385.1"
                     /translation="MNKPFIAIEGPIGVGKSSLAHKLSQTLDFYEEKEIITENPFLSD
                     FYEDISKWSFQTEMFFLCNRYKQFQDVTQLNQGVVSDYHIHKNKIFAKNTLSSVEFQK
                     FSKIYDILTEDMIMPNMIIFLDADLDVLKSRIAKRNRSFEHQIEDEYLLKLKKDYREY
                     YESLQSNGSNVVLIDTTSIDFLKNEQDYEDILHIILPMIGDITNE"
     gene            2997..3659
                     /locus_tag="O2701_000476"
     CDS             2997..3659
                     /locus_tag="O2701_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCZ4292386.1"
                     /translation="MNNYGIPQNAIITIAGTVGVGKSTLTQALADKLNFKTSFENVEH
                     NPYLDKFYSDFERWSFHLQIYFLAERFKEQKRMFEYGGGFVQDRSIYEDVDIFAKMHE
                     EEGTMSKEDFKTYSDLFNAMVMTPYFPKPDVMIYLECNYDEVIDRIIERGREMEINTD
                     PEYWKKLFKRYDDWINSFNACPVVRININEYDIHKDPESLNPMINKIARIIQTYRQVD
                     TR"
     gene            complement(3775..4458)
                     /locus_tag="O2701_000477"
     CDS             complement(3775..4458)
                     /locus_tag="O2701_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCZ4292387.1"
                     /translation="MEWILFDKDGTLIEFDRSWEKIGVRFVQSLLETFPVHNKEAALR
                     QLGVIKESIDPKSVMGSGSLQQIIQAFNDVTGQDTTDWSKSTSQKLVDERIPEINWVE
                     GVKEALIDLKAKGYQLGIVTSDTKKGVEQFLAHTNATSLFDLIISTEADAYEKPNPKV
                     LSPLFEQYNVDPQKVAIVGDTANDMKTASNANLGMAIGVLTGIATKEELHEADIILNS
                     AADILEALN"
     gene            complement(4708..5784)
                     /locus_tag="O2701_000478"
     CDS             complement(4708..5784)
                     /locus_tag="O2701_000478"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HCZ4292388.1"
                     /translation="MSQAVKVERRETLKQKPNTSQLGFGKYFTDYMLSYDYDADKGWH
                     DLKIVPYGPIEISPAAQGVHYGQSVFEGLKAYKRDGEVALFRPEENFKRLNNSLARLE
                     MPQVDEAELLEGLKQLVDIERDWIPEGEGQSLYIRPFVFATEGALGVGASHQYKLLII
                     LSPSGAYYGGETLKPTKIYVEDEYVRAVRGGVGFAKVAGNYAASLLAQTNANKLGYDQ
                     VLWLDGVEQKYIEEVGSMNIFFVENGKVITPELNGSILPGITRKSIIELAKNLGYEVE
                     ERRVSIDELFESYDKGELTEVFGSGTAAVISPVGTLRYEDREIVINNNETGEITQKLY
                     DVYTGIQNGTLEDKNGWRVVVPKY"
     gene            complement(6119..7084)
                     /locus_tag="O2701_000479"
     CDS             complement(6119..7084)
                     /locus_tag="O2701_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCZ4292389.1"
                     /translation="MKKIMITGALGQIGTELVVKCREIYGTDNVLATDIREPEADSPV
                     QNGPFEILDVTDRDRMFELVRDFEADSLMHMAALLSATAEKNPILAWDLNMGGLMNAL
                     EAARTYNLHFFTPSSIGAFGDSTPKVNTPQVTIQQPTTMYGVNKVAGELLCQYYFKRF
                     GVDTRSVRFPGLISHVKEPGGGTTDYAVEIYFKAVREGHYTSFIDKGTYMDMMYMDDA
                     IEAIIKLMEADDAKLETRNGYNLSAMSFDPEMVKEAIQEYYPNFTLDYDVDPIRQGIA
                     NSWPDSIDTSCSRGEWGFDPKYDLASMTKLMLEAIEQKDTVKNNN"
     gene            complement(7298..8935)
                     /locus_tag="O2701_000480"
     CDS             complement(7298..8935)
                     /locus_tag="O2701_000480"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCZ4292390.1"
                     /translation="MSYSIGIDYGTASGRVFLINTTNGQVVSKFVKPYTHGVIESELN
                     GLKIPHTYALQNSNDYLEIMEEGISYIVRESKIDPDNIVGIGIDFTSSTIIFTDENLN
                     PVHNLKQFKNNPHAYVKLWKHHGAYKEAEKLYQTAIENNNKWLGHYGYNVSSEWMIPK
                     IMEVMNRAPEIMEKTAYIMEAGDWIVNKLTNKNVRSNCGLGFKAFWEEETGFHYDLFD
                     KIDPKLSKVIQDKVSAPVVNIGEAVGKLDDKMAQKLGLSKETMVSPFIIDAHASLLGI
                     GSEKDKEMTMVMGTSTCHLMLNEKQHQVPGISGSVKGAIIPELFAYEAGQSAVGDLFE
                     YVAKQAPKSYVDEAENRNMTVFELMNEKIKHQMPGESGLIALDWHNGNRSVLSDSNLT
                     GCIFGLTLQTKHEDIYRAYLEATAFGTKMIMQQYQDWHMEVEKVFACGGIPKKNAVMM
                     DIYANVLNKKLIVMDSEYAPAIGAAILGAVSGGAHNSINDAVDAMKEPILYEINPEAE
                     KVQRYETLFKAYKALHDIHGYKKANIMKDIQSLRVEG"
     gene            complement(9096..9974)
                     /gene="hchA"
                     /locus_tag="O2701_000481"
     CDS             complement(9096..9974)
                     /gene="hchA"
                     /locus_tag="O2701_000481"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCZ4292391.1"
                     /translation="MSQDVNELSKQPTPDKAEDNAFFPSPYSLSQYTAPKTDFDGVEH
                     KGAYKDGKWKVLMIAAEERYVLLENGKMFSTGNHPVEMLLPLHHLMEAGFDVDVATLS
                     GYPVKLELWAMPTEDEAVISTYNKLKEKLKQPKKLADVIKNELGPDSDYLSVFIPGGH
                     AAVVGISESEDVQQTLDWALDNDRFIVTLCHGPAALLSAGLNREKSPLEGYSVCVFPD
                     SLDEGANIEIGYLPGRLKWLVADLLTKQGLKVVNDDMTGRTLKDRKLLTGDSPLASNE
                     LGKLAVNEMLNAIQNK"
     gene            complement(10240..11427)
                     /locus_tag="O2701_000482"
     CDS             complement(10240..11427)
                     /locus_tag="O2701_000482"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCZ4292392.1"
                     /translation="MVQSLHEFLEENINYLKENGLYNEIDTIEGANGPEIKINGKSYI
                     NLSSNNYLGLATNEDLKSAAKAAIDTHGVGAGAVRTINGTLDLHDELEETLAKFKGTE
                     AAIAYQSGFNCNMAAISAVMNKNDAILSDELNHASIIDGCRLSKAKIIRVNHSDMDDL
                     RAKAKEAVESGQYNKVMYITDGVFSMDGDVAKLPEIVEIAEEFGLLTYVDDAHGSGVM
                     GKGAGTVKHFGLQDKIDFQIGTLSKAIGVVGGYVAGTKELIDWLKAQSRPFLFSTSLA
                     PGDTKAITEAVKKLMDSTELHDKLWNNAQYLKNGLSKLGYDTGESETPITPVIIGDEK
                     TTQEFSKRLKDEGVYVKSIVFPTVPRGTGRVRNMPTAAHTKDMLDEAIAAYEKVGKEM
                     KLI"
     gene            11598..12773
                     /locus_tag="O2701_000483"
     CDS             11598..12773
                     /locus_tag="O2701_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCZ4292393.1"
                     /translation="MLDWFQLANNKENKTIQLRRYLHQYPELSFEEFQTHDYIVNQLS
                     QLSCDIETPIGRNGIKATFKGLGTGPTIALRADFDALPVEELNDVPYKSKNPGCMHAC
                     GHDGHTAILLTVAEILDEHKHLLEGNVVLIFQYGEEIMPGGSQEMIDAGCLENVDRIY
                     GTHLWSGYPTGTIHSRAGAIMASPDEFSVTFKGRGGHGAKPHETIDPIVIMAEFILSA
                     QKIISRTIDPVKQAVLSFGMIQAGTTDSVIPDQAFCKGTVRTFDSDIQNHVMDKMDKL
                     LQGLAIANDINYDLNYIKGYLPVHNNEKAYQVIKEATNDLHVRFNESDLMMIGEDFSH
                     YLKVRPGAFFLTGCGNESKGITAPHHNPKFDIDEKSLKYAVAVFLKIIELEQVFKTN"
     gene            complement(13055..14239)
                     /gene="tuf"
                     /locus_tag="O2701_000484"
     CDS             complement(13055..14239)
                     /gene="tuf"
                     /locus_tag="O2701_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCZ4292394.1"
                     /translation="MAKEKFDRSKEHANIGTIGHVDHGKTTLTAAIATVLAKNGDSVA
                     QSYDMIDNAPEEKERGITINTSHIEYQTDKRHYAHVDCPGHADYVKNMITGAAQMDGG
                     ILVVSAADGPMPQTREHILLSRNVGVPALVVFLNKVDMVDDEELLELVEMEVRDLLSE
                     YDFPGDDVPVIAGSALKALEGDAQYEEKILELMEAVDTYIPTPERDSDKPFMMPVEDV
                     FSITGRGTVATGRVERGQIKVGEEVEIIGLHDTSKTTVTGVEMFRKLLDYAEAGDNIG
                     ALLRGVAREDVQRGQVLAAPGSITPHTEFKAEVYVLSKDEGGRHTPFFSNYRPQFYFR
                     TTDVTGVVHLPEGTEMVMPGDNVEMTVELIAPIAIEDGTRFSIREGGRTVGSGVVTEI
                     IK"
     gene            complement(14456..16537)
                     /gene="fusA"
                     /locus_tag="O2701_000485"
     CDS             complement(14456..16537)
                     /gene="fusA"
                     /locus_tag="O2701_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCZ4292395.1"
                     /translation="MAREFSLEKTRNIGIMAHIDAGKTTTTERILYYTGRIHKIGETH
                     EGASQMDWMEQEQDRGITITSAATTAAWEGHRVNIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFVNKMDKLGANFEYSVSTLHDRLQANAA
                     PIQLPIGAEDEFEAIIDLVEMKCFKYTNDLGTEIEEIEIPEDHLDRAEEARASLIEAV
                     AETSDELMEKYLGDEEISVSELKEAIRQATTNVEFYPVLCGTAFKNKGVQLMLDAVID
                     YLPSPLDVKPIIGHRASNPEEEVIAKADDSAEFAALAFKVMTDPYVGKLTFFRVYSGT
                     MTSGSYVKNSTKGKRERVGRLLQMHANSRQEIDTVYSGDIAAAVGLKDTGTGDTLCGE
                     KNDIILESMEFPEPVIHLSVEPKSKADQDKMTQALVKLQEEDPTFHAHTDEETGQVII
                     GGMGELHLDILVDRMKKEFNVECNVGAPMVSYRETFKSSAQVQGKFSRQSGGRGQYGD
                     VHIEFTPNETGAGFEFENAIVGGVVPREYIPSVEAGLKDAMENGVLAGYPLIDVKAKL
                     YDGSYHDVDSSEMAFKIAASLALKEAAKKCDPVILEPMMKVTIEMPEEYMGDIMGDVT
                     SRRGRVDGMEPRGNAQVVNAYVPLSEMFGYATSLRSNTQGRGTYTMYFDHYAEVPKSI
                     AEDIIKKNKGE"
     gene            complement(16660..17130)
                     /gene="rpsG"
                     /locus_tag="O2701_000486"
     CDS             complement(16660..17130)
                     /gene="rpsG"
                     /locus_tag="O2701_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCZ4292396.1"
                     /translation="MPRKGSVPKRDVLPDPIHNSKLVTKLINKIMLDGKRGTAQRILY
                     SAFDLVEQRSGRDALEVFEEAINNIMPVLEVKARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNYARLRGEKTMEDRLANEILDAANNTGGAVKKREDTHKMAEANKAFAHYRW"
     gene            complement(17196..17609)
                     /gene="rpsL"
                     /locus_tag="O2701_000487"
     CDS             complement(17196..17609)
                     /gene="rpsL"
                     /locus_tag="O2701_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCZ4292397.1"
                     /translation="MPTINQLVRKPRQSKIKKSDSPALNKGFNSKKKKFTDLNSPQKR
                     GVCTRVGTMTPKKPNSALRKYARVRLSNNIEINAYIPGIGHNLQEHSVVLVRGGRVKD
                     LPGVRYHIVRGALDTSGVDGRRQGRSLYGTKKPKN"
     gene            complement(17707..17961)
                     /locus_tag="O2701_000488"
     CDS             complement(17707..17961)
                     /locus_tag="O2701_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HCZ4292398.1"
                     /translation="MSKEKVARFNKQHFVVGLKETLKALKKDQVTSLIIAEDVEVYLM
                     TRVLSQINQKNIPVSFFKSKHALGKHVGINVNATIVALIK"
     gene            complement(18098..21694)
                     /gene="rpoC"
                     /locus_tag="O2701_000489"
     CDS             complement(18098..21694)
                     /gene="rpoC"
                     /locus_tag="O2701_000489"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCZ4292399.1"
                     /translation="MKIGLASPEKIRSWSFGEVKKPETINYRTLKPEKDGLFCERIFG
                     PTKDWECSCGKYKRVRYKGMVCDRCGVEVTKSKVRRERMGHIELAAPVSHIWYFKGIP
                     SRMGLLLDMSPRALEEVIYFASYVVVDPGPTGLEKKTLLSEAEFRDYYDKYPGQFVAK
                     MGAEGIKDLLEEIDLDEELKLLRDELESATGQRLTRAIKRLEVVESFRNSGNKPSWMI
                     LDVLPIIPPEIRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPGIIVQNEK
                     RMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGKRVDYSGRSV
                     IAVGPSLKMYQCGLPKEMALELFKPFVMKELVQREIATNIKNAKSKIERMDDEVWDVL
                     EEVIREHPVLLNRAPTLHRLGIQAFEPTLVEGRAIRLHPLVTTAYNADFDGDQMAVHV
                     PLSKEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTLERKDAVNTGAIFN
                     NTNEVLKAYANGFVHLHTRIGVHASSFNNPTFTEEQNKKILATSVGKIIFNEIIPDSF
                     AYINEPTQENLERKTPNRYFIDPTTLGEGGLKEYFENEELIEPFNKKFLGNIIAEVFN
                     RFSITDTSMMLDRMKDLGFKFSSKAGITVGVADIVVLPDKQQILDEHEKLVDRITKQF
                     NRGLITEEERYNAVVEIWTDAKDQIQGELMQSLDKTNPIFMMSDSGARGNASNFTQLA
                     GMRGLMAAPSGKIIELPITSSFREGLTVLEYFISTHGARKGLADTALKTADSGYLTRR
                     LVDVAQDVIVREEDCGTDRGLLVSDIKEGTEMIEPFIERIEGRYSKETIHHPETDEII
                     IRPDELITPEIAKKITDAGIEQMYIRSAFTCNARHGVCEKCYGKNLATGEKVEVGEAV
                     GTIAAQSIGEPGTQLTMRTFHTGGVAGSDITQGLPRIQEIFEARNPKGQAVITEIEGV
                     VEDIKLAKDRQQEIVVKGANETRSYLASGTSRIIVEIGQPVQRGEVLTEGSIEPKNYL
                     SVAGLNATESYLLKEVQKVYRMQGVEIDDKHVEVMVRQMLRKVRIIEAGDTKLLPGSL
                     VDIHNFTDANREAFKHRKRPATAKPVLLGITKASLETESFLSAASFQETTRVLTDAAI
                     KGKRDDLLGLKENVIIGKLIPAGTGMRRYSDVKYEKTAKPVAEVESQTEVTE"
     gene            complement(21858..25409)
                     /gene="rpoB"
                     /locus_tag="O2701_000490"
     CDS             complement(21858..25409)
                     /gene="rpoB"
                     /locus_tag="O2701_000490"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCZ4292400.1"
                     /translation="MAGQVVQYGRHRKRRNYARISEVLELPNLIEIQTKSYEWFLREG
                     LIEMFRDISPIEDFTGNLSLEFVDYRLGEPKYDLEESKNRDATYAAPLRVKVRLIIKE
                     TGEVKEQEVFMGDFPLMTDTGTFVINGAERVIVSQLVRSPSVYFNEKIDKNGRENYDA
                     TIIPNRGAWLEYETDAKDVVYVRIDRTRKLPLTVLLRALGFSSDQEIVDLLGDNEYLR
                     NTLEKDGTENTEQALLEIYERLRPGEPPTVENAKSLLYSRFFDPKRYDLASVGRYKTN
                     KKLHLKHRLFNQKLAEPIVNTETGEIVVEEGTVLDRRKIDEIMDVLESNANSEVFELH
                     GSVIDEPVEIQSIKVYVPNDDEGRTTTVIGNAFPDSEVKCITPADIIASMSYFFNLLS
                     GIGYTDDIDHLGNRRLRSVGELLQNQFRIGLSRMERVVRERMSIQDTESITPQQLINI
                     RPVIASIKEFFGSSQLSQFMDQANPLAELTHKRRLSALGPGGLTRERAQMEVRDVHYS
                     HYGRMCPIETPEGPNIGLINSLSSYARVNEFGFIETPYRKVDLDTHAITDQIDYLTAD
                     EEDSYVVAQANSKLDENGRFMDDEVVCRFRGNNTVMAKEKMDYMDVSPKQVVSAATAC
                     IPFLENDDSNRALMGANMQRQAVPLMNPEAPFVGTGMEHVAARDSGAAITAKHRGRVE
                     HVESNEILVRRLVEENGVEHEGELDRYPLAKFKRSNSGTCYNQRPIVAVGDVVEYNEI
                     LADGPSMELGEMALGRNVVVGFMTWDGYNYEDAVIMSERLVKDDVYTSIHIEEYESEA
                     RDTKLGPEEITRDIPNVSESALKNLDDRGIVYIGAEVKDGDILVGKVTPKGVTELTAE
                     ERLLHAIFGEKAREVRDTSLRVPHGAGGIVLDVKVFNREEGDDTLSPGVNQLVRVYIV
                     QKRKIHVGDKMCGRHGNKGVISKIVPEEDMPYLPDGRPIDIMLNPLGVPSRMNIGQVL
                     ELHLGMAAKNLGIHVASPVFDGANDDDVWSTIEEAGMARDGKTVLYDGRTGEPFDNRI
                     SVGVMYMLKLAHMVDDKLHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGA
                     AYTLQEILTYKSDDTVGRVKTYEAIVKGENISRPSVPESFRVLMKELQSLGLDVKVMD
                     EQDNEIEMTDVDDDDVVERKVDLQQNDAPETQKEVTD"
     gene            complement(25624..26232)
                     /locus_tag="O2701_000491"
     CDS             complement(25624..26232)
                     /locus_tag="O2701_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCZ4292401.1"
                     /translation="MSHYYDEDPSVISNEQRIQYQLNHHKIDLITDNGVFSKDKVDYG
                     SDVLVQTFLKAHPPGPSKRIADVGCGYGPIGLMIAKVSPHHSITMLDVNHRALALVEK
                     NKKLNGIDNVIVKESDALSAVEDKSFDFILTNPPIRAGKETVHRIFEQALHRLDSNGE
                     LFVVIQKKQGMPSAKKRMNELFGNVEVVNKDKGYYILRSIKA"
     gene            complement(26407..26775)
                     /gene="rplL"
                     /locus_tag="O2701_000492"
     CDS             complement(26407..26775)
                     /gene="rplL"
                     /locus_tag="O2701_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCZ4292402.1"
                     /translation="MANHEQIIEAIKEMSVLELNDLVKAIEEEFGVTAAAPVAVAGAA
                     GGADAAAEKTEFDVELTSAGSSKIKVVKAVKEATGLGLKDAKELVDGAPKVIKEALPK
                     EEAEKLKEQLEEVGATVELK"
     gene            complement(26818..27318)
                     /locus_tag="O2701_000493"
     CDS             complement(26818..27318)
                     /locus_tag="O2701_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCZ4292403.1"
                     /translation="MSAIIEAKKQLVDEIAEVLSNSVSTVIVDYRGLTVAEVTDLRSQ
                     LREAGVEYKVYKNTMVRRAAEKAGIEGLDEFLTGPTAIATSSEDAVAAAKVISGFAKD
                     HEALEIKSGVMEGNVITAEEVKTVGSLPSHDGLVSMLLSVLQAPVRNFAYAVKAIGEQ
                     KEENAE"
     misc_feature    complement(27356..27502)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(27590..28282)
                     /gene="rplA"
                     /locus_tag="O2701_000494"
     CDS             complement(27590..28282)
                     /gene="rplA"
                     /locus_tag="O2701_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCZ4292404.1"
                     /translation="MAKKGKKYQEAASKVDRTQHYSVEEAIKLAKETSIANFDASVEV
                     AFRLGIDTRKNDQQIRGAVVLPNGTGKSQSVLVFAKGDKIAEAEAAGADYVGEAEYVQ
                     KIQQGWFDFDVVVATPDMMGEVGKLGRVLGPKGLMPNPKTGTVTMDVKKAVEEIKAGK
                     VEYRAEKAGIVHASIGKVSFTDEQLIENFNTLQDVLAKAKPSSAKGTYFKSVAVTTTM
                     GPGVKIDTASFK"
     gene            complement(28490..28912)
                     /gene="rplK"
                     /locus_tag="O2701_000495"
     CDS             complement(28490..28912)
                     /gene="rplK"
                     /locus_tag="O2701_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012664221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCZ4292405.1"
                     /translation="MAKKVDKVVKLQIPAGKANPAPPVGPALGQAGVNIMGFCKEFNA
                     RTQDQAGLIIPVEISVYEDRSFTFITKTPPAPVLLKKAAGIEKGSGEPNKTKVATVTK
                     DQVREIANSKMQDLNAADEEAAMRIIEGTARSMGIVVE"
     gene            complement(29093..29641)
                     /gene="nusG"
                     /locus_tag="O2701_000496"
     CDS             complement(29093..29641)
                     /gene="nusG"
                     /locus_tag="O2701_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCZ4292406.1"
                     /translation="MSEEVGAKRWYAVHTYSGYENKVKKNLEKRVESMNMTEQIFRVV
                     IPEEEETQVKDGKAKTTVKKTFPGYVLVELIMTDESWYVVRNTPGVTGFVGSAGAGSK
                     PNPLLPEEVRFILKQMGLKEKTIDVELEVGEQVRIKSGPFANQVGEVQEIETDKFKLT
                     VLVDMFGRETPVEVEFDQIEKL"
     gene            complement(29654..29836)
                     /gene="secE"
                     /locus_tag="O2701_000497"
     CDS             complement(29654..29836)
                     /gene="secE"
                     /locus_tag="O2701_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCZ4292407.1"
                     /translation="MAKKESFFKGVKSEMEKTSWPTKEELFKYTVIVVSTVIFFLVFF
                     YALDLGITALKNLLFR"
     gene            complement(29892..30035)
                     /gene="rpmG"
                     /locus_tag="O2701_000498"
     CDS             complement(29892..30035)
                     /gene="rpmG"
                     /locus_tag="O2701_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002451211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCZ4292408.1"
                     /translation="MRKIPLNCEACGNRNYNVPKQEGSATRLTLKKYCPKCNAHTIHK
                     ESK"
     gene            complement(30150..30719)
                     /locus_tag="O2701_000499"
     CDS             complement(30150..30719)
                     /locus_tag="O2701_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HCZ4292409.1"
                     /translation="MKYDLTTQDSTIKRNNAINDKDFEKLVMDLQPLIIRRIKTFGFN
                     HYDLEDLYQEILIRMYRSVQTFDFSGEQPFTNYVQCLITSVKYDYLRKYLATNKRMDN
                     LINEYRVTYPCAIKRFDVENNYLNKLAIKELICQFKYLSAFEKDVMYLMCEQYKPREI
                     AQLMHVKEKVIYNAIQRCKNKIKRYFKMI"
     gene            complement(30800..31324)
                     /locus_tag="O2701_000500"
     CDS             complement(30800..31324)
                     /locus_tag="O2701_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HCZ4292410.1"
                     /translation="MKERYLIIDGYNMIGQSPTLSAIAKENLEEARMQLIDAIANYNA
                     VISDEIICVFDAYDQSGVEREYMYHGVKTIFTKEKETADSFIERYVYELYDKHTKHIT
                     VVTSDMSEQHAIFGSGAYRISSREMWRDLKENEIDVSKSLDDISENKPRTRIPLSSEI
                     LAEFEKIRRGHHKK"
     gene            complement(31324..32070)
                     /gene="rlmB"
                     /locus_tag="O2701_000501"
     CDS             complement(31324..32070)
                     /gene="rlmB"
                     /locus_tag="O2701_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCZ4292411.1"
                     /translation="MEDTVIVGRHAVREAIITGHPINKILIQEGIKKQQINEILKNAK
                     DQKIIVQTVPKSKLDFLANAPHQGVAALIAPYEYADFDQFLKQQKEKEGLLTVLILDG
                     LEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVIRVTNLAKT
                     IDELKDNGFWVAGTEANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCDFYIKIPM
                     VGHVNSLNASVAASLMMYEVFRKRHDVGEI"
     gene            complement(32078..32482)
                     /locus_tag="O2701_000502"
     CDS             complement(32078..32482)
                     /locus_tag="O2701_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HCZ4292412.1"
                     /translation="MDNQQDNHIKLLNPLTLAYMGDAVLDQYVRTYIVLKLKSKPNKL
                     HQMSKKYVSAKSQAQTLEYLMEQEWFTDEEMDILKRGRNAKSHTKAKNTDVQTYRKSS
                     AIEAVIGFLYLEKREERLEALLNKIITIVNER"
     gene            complement(32475..33875)
                     /locus_tag="O2701_000503"
     CDS             complement(32475..33875)
                     /locus_tag="O2701_000503"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCZ4292413.1"
                     /translation="MITLYNTLTRQKEVFKPIEPGKVKMYVCGPTVYNYIHIGNARPA
                     INYDVVRRYFEYQGYNVEYVSNFTDVDDKLIKRSQELNQSVPEIAEKYIAAFHEDVGA
                     LNVRKATSNPRVMDHMDDIIQFIKDLVDQGYAYESGGDVYFRTRKFEGYGKLSHQSID
                     DLKVGARIDAGEHKEDALDFTLWKKAKPGEISWDSPFGEGRPGWHIECSVMAFHELGP
                     TIDIHAGGSDLQFPHHENEIAQSEAHNHAPFANYWMHNGFINIDNEKMSKSLGNFILV
                     HDIIKEVDPDVLRFFMISVHYRSPINYNLELVESARSGLERIRNSYQLIEERAQIATN
                     IENQQTYIDQIDAILNRFETVMNDDFNTANAITAWYDLAKLANKYVLENTTSTEVIDK
                     FKAVYQIFSDVLGVPLKSKNADELLDEDVEKLIEERNEARKNKDFARADEIRDMLKSQ
                     NIILEDTPQGVRFKRG"
     gene            complement(33859..34506)
                     /gene="cysE"
                     /locus_tag="O2701_000504"
     CDS             complement(33859..34506)
                     /gene="cysE"
                     /locus_tag="O2701_000504"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCZ4292414.1"
                     /translation="MILLKRMRDDIKMVFEQDPAARSTLEVITTYAGLHAVWSHLIAH
                     KLYNQKKYVAARAISQISRFFTGIEIHPGAKIGKRLFIDHGMGVVIGETCTIGDNVTI
                     YQGVTLGGTGKERGKRHPDIGDNVLIAAGAKVLGNIKINSNVNIGANSVVLQSVPSYS
                     TVVGIPGHIVKQDGVRVGKTFDHRHLPDPIYEQIKHLERQLEKTRNGEIQDDYII"
     gene            complement(34929..36383)
                     /locus_tag="O2701_000505"
     CDS             complement(34929..36383)
                     /locus_tag="O2701_000505"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCZ4292415.1"
                     /translation="MSDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIE
                     DTDKKRNLEDGETSQFDNLKWLGLDWDESVDKDNGYGPYRQSERQHIYQPLIDQLLAE
                     DKAYKCYMTEEELEAEREAQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVP
                     QNQTYSFDDMVKGNISFDSNGIGDWVIVKKDGIPTYNFAVAIDDHYMQISDVIRGDDH
                     ISNTPKQIMIYEAFGWEPPRFGHMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEA
                     LFNFIALLGWSPEGEEEIFSKEEFIKIFDEKRLSKSPAFFDKQKLAWVNNQYMKQKDT
                     ETVFQLALPHLIKANLIPEVPSEEDLSWGRKLIALYQKEMSYAGEIVPLSEMFFKEMP
                     ALGEEEQQVINGEQVPELMTHLFSKLEALEPFEAAEIKKTIKEVQKETGIKGKQLFMP
                     IRVAVTGQMHGPELPNTIEVLGKEKVLNRLKQYK"
BASE COUNT        11074 a         7108 c         5414 g        13134 t
ORIGIN      
        1 atactttttt tctttaattg ctttaaatta tttatctata tttttaaaac tcacttttaa
       61 tctaagcttg ttttcaattc gctattacat cttattttaa ttaataacat tgctcacaac
      121 agtaatatca aaggcttact attggtgcat tagaacaaaa aataggcatg tgacttaaaa
      181 gcattctcaa tgaaatacct taagctacat acctattttt aatgtatatt tacatatttt
      241 ttaatattat tttaaatgtt catatggact attattcaca aaagatacaa tttgatcgac
      301 aaataatctt gctcgtaatt ggaattcact atcatttaag taatatgaac catcttcatt
      361 tttagcaaaa tcagaatcat gtgttgctat ttgagttggt acagcaatgc ctagtaaact
      421 tctgacgatt actcttaaat gtgacaatgg ctctgaacta acaataccac cactatttcc
      481 tattaagcct acaggtttca ttttaaaata atccatattt agatgatcta atgcattttt
      541 caatattcca gaatatgaac catgatagtt tggcgttcct aatattaaaa agtccgccgc
      601 cattgctttc tcttttaaat ctttcatatt ttgtttgatt tcatcaatag acggtgttgt
      661 tcctgaaaaa tctaattgat ttaacggttt ttctgctaaa tcgaatattt ccgcttcaat
      721 atcatgtgtt ttaaaatgct cagttaagta tcttgctagt gcacttgtat gtgaattcac
      781 ttgtgcactg ccaataataa ttaatccttt catatttgac ccctcttttt ttattaaact
      841 tgttgttcac gtaataattt cataatagct tttccaacac cactattttc attcgtatct
      901 gtgacatatt tcgctatttt tttaacttct tctgcaccat tttccatcgc aactggatag
      961 ccaactttct ctaacattga taagtcattt aaattgtcac ctattgacat gacttctttc
     1021 atttcaatcc ctaatctttc ggcaattgtt tctagcgcaa tacctttttg tgcatctgaa
     1081 tgcgttattt ctatatttcc tctcgaagat gatgatatag ctaaattcgg agattcagct
     1141 aaaattttac tagctttgtc aattttttct aaatttccat caaatgctaa tattttcata
     1201 attaattcac caggtatgtt ttcaatagca tcataattat caacaacttt caacgtacca
     1261 ttatctatgc gtctttgaat accattttta atacgctcaa cgtttgcatg ttgacctgca
     1321 cgctcagcaa tatctatgta aatgtctaaa tctctttgtg gatcttcagt ataaatcgca
     1381 cgactcgtgt atacttgata ataaatacct gcatctttta aaacatttgt aattttgtgt
     1441 actaacgatt tattaaggtg tgaagtgctc attacattga aagtttcatc acgtacttca
     1501 gcaccattca aacaaatata tggtactgtt aaatctgtgt cagcaactgg tgcttgtgct
     1561 tcataaaatg ctcgacctgt cgcgataaca accgttatcc cttgttcttg agcgtattta
     1621 atcgcatcaa tattaggttg agaaatttca tgtgctgcat taagtagcgt gccatccata
     1681 tcagtggcta ttagttttat cattatgtta cctcgtttcg taaatttaaa atcttgttct
     1741 taaataagaa tatatactca gcgcacatac ttttctatta acatttatat tgtcattaat
     1801 ttatcatata atgtaattct aacaaatttt aattagtatg tactatcgtc taattggtgg
     1861 atttcttatt ggctcttaag tttttaaaaa atgttgttaa taatgtgcta catgcttctt
     1921 taagtacacc tttatcaaca attgcacgat gattaaaatt agattgttgc aataaattca
     1981 ttaaactgcc actacaacca cctttaggat catctgcgcc atagacgact cttggaatgc
     2041 gactcattac aattgttcct gcgcacatga cacatggttc taaggttaca tataatgtgc
     2101 aaccttctaa acgccaacta cctaacactt tggctgcacg ttcaattgca atatgttcag
     2161 catgcgccgt tggttgttgt agtgtttctc ttaaattatg tgctctagcg ataacttcat
     2221 catctttagt gatgatagca cctataggta cttcgcctag ttgagctgct tttttagctt
     2281 cttcaatcgc taatgtcata aaatatatat catttgtcat ttatgtccag atacctcact
     2341 tatggtacaa tactcaatgt ctattttaac atttggagat gtgaatatga acaaaccttt
     2401 tatagcaatt gaaggtccta ttggcgtagg taaatcttca cttgcacaca aattaagtca
     2461 aactttagat ttttatgaag aaaaagaaat catcacagaa aatccatttt tatcagactt
     2521 ttatgaagat atctctaaat ggagttttca aactgaaatg ttctttttat gcaatagata
     2581 taagcaattt caagatgtaa cacaactaaa tcaaggtgta gttagtgatt atcatataca
     2641 taaaaataag atatttgcta aaaatacttt gagttctgtt gaatttcaga aattcagtaa
     2701 aatttatgat attttaactg aagatatgat tatgccgaat atgattatct ttttagatgc
     2761 agaccttgat gtgttaaaat ctagaattgc taaacgtaac cgtagttttg agcatcaaat
     2821 agaagatgaa tatctgttaa agttaaaaaa agattatcgt gagtattatg agtccttaca
     2881 aagtaatggt tcaaatgtag ttttaatcga tacaacttct attgattttc ttaaaaatga
     2941 acaagattac gaagatatat tacatattat attacctatg ataggagata ttaccaatga
     3001 ataattacgg tattccacaa aatgccatta taaccattgc aggtacagtt ggtgttggaa
     3061 aatcaacact aacgcaagca cttgcagata aattaaactt taaaacgtct tttgaaaatg
     3121 tcgaacataa tccatattta gataaatttt acagcgattt tgaacgatgg agtttccatt
     3181 tgcaaattta cttcttagct gaacgtttta aagaacaaaa gcgtatgttt gaatatggtg
     3241 gtggctttgt ccaagatcga tcaatttatg aagatgttga tatttttgca aaaatgcatg
     3301 aagaagaagg cacaatgagt aaagaagatt tcaaaacata ttcagactta tttaatgcca
     3361 tggtcatgac accttatttt cctaaacctg atgtaatgat ttatttagaa tgtaactatg
     3421 atgaggtcat tgatcgtatt attgaacgtg gtcgcgaaat ggaaattaat acagaccctg
     3481 aatactggaa aaagctattt aaacgctatg acgattggat taatagcttt aatgcatgtc
     3541 cagttgtacg tatcaatatt aatgaatatg atatccataa ggaccccgaa tctttaaatc
     3601 ctatgataaa caaaattgct cgaattattc aaacatatcg acaagtagat acacgataaa
     3661 agactaaaga catagcgtat atgtttatat tcaatgtata ttccatagat attatcgatt
     3721 attttatcaa ttctatcgaa tacattaatt cacatataca ctatgtcttt ctttttaatt
     3781 taaagcttct aaaatatctg ccgcactatt taaaataata tcagcttcat gtaattcttc
     3841 ttttgttgca atacctgtta atacacctat tgccatacct aaatttgcat tacttgctgt
     3901 cttcatatca ttagcagtgt ctcctactat agctactttc tgaggatcta cattatattg
     3961 ctcaaataaa ggcgataata ctttaggatt tggcttctca taggcatccg cttcggtaga
     4021 aatgatcaaa tcgaacaacg aggtagcatt ggtatgtgct aaaaattgtt ctacaccttt
     4081 tttagtatca ctcgtaacaa taccaagttg atagcctttt gctttcaaat cgataagtgc
     4141 ttctttaaca ccttctaccc aattaatttc aggaatacgt tcatctacca gcttttgact
     4201 tgttgacttg gaccagtcgg ttgtatcttg tcccgtcaca tcattaaatg cctggataat
     4261 ttgttgtaaa gatcctgaac ccatcactga ttttggatca atagattctt taatgacacc
     4321 gagttgtctt aaagcagctt ctttattatg tactgggaaa gtctcaagca atgattgtac
     4381 aaatcgtacc cctatttttt cccaacttct atcaaattca attaacgtac catctttatc
     4441 aaataatatc cattccattg atatcaatac tcctatttat ttatttcgta ttatgctgat
     4501 tctatgatat tcgttatccc ctgaaaatga actcgtagta ttgttctatt taaatattga
     4561 attaaatata ataataagtg aaatcccctt caatacttaa caataaacat tgtaaactta
     4621 atttattacc atgcttcgct tcattgaaag ggattttagt catgattaac ttttgcatat
     4681 tgttttcatg attatattca atttttatta atattttggt acaacgactc tccaaccatt
     4741 tttatcttct aaagtaccat tttgaatacc agtatagacg tcgtataatt tttgagtaat
     4801 ttcaccagtc tcattattat taataacgat ttcacgatct tcgtatctca atgtacccac
     4861 aggtgaaata actgctgcag taccactacc aaatacttct gttaactcac ctttatcata
     4921 tgattcgaat aattcatcga ttgaaacgcg gcgctcttcg acttcatatc ctaagttttt
     4981 agctaattcg ataatagatt tacgtgtaat accaggtaaa atactgccat tcaactctgg
     5041 tgtaattact ttgccatttt caacgaagaa aatgttcatg ctaccaactt cttcgatata
     5101 tttctgttca acaccatcaa gccataatac ttggtcataa cctaatttat ttgcattagt
     5161 ttgtgctaat aaacttgccg catagttacc tgcaactttt gcaaagccta caccgccacg
     5221 aacagcacgc acatattcat cttctacata gattttagtt ggttttaaag tttcaccacc
     5281 ataatatgca cctgaaggag ataaaataat taataattta tactgatgtg atgcaccaac
     5341 gccaagtgcc ccttctgttg caaaaacaaa tggacgaata tataatgatt gaccttcccc
     5401 ttcaggaatc caatctcttt caatatcaac taattgtttt agcccctcta acaattctgc
     5461 ttcgtctact tgaggcattt ctaatcgtgc taacgagtta ttaagacgct taaaattttc
     5521 ttcaggacgg aaaagtgcaa cttccccatc tcttttatat gcttttaatc cttcgaatac
     5581 cgattgacca taatgaacac cttgtgcagc aggtgaaatt tcaataggac cataaggtac
     5641 tatcttcaaa tcatgccatc ctttatctgc atcataatca taactcaaca tataatcagt
     5701 aaaatattta ccaaaaccta gttgagatgt atttggtttt tgttttaatg tttctcgtcg
     5761 ttcaacttta actgcttgtg acatggtgat tgcctcctaa taatattgta taagaatttg
     5821 tttaacttaa attataacaa tccatatttt gctgttcaac aaattttcta aaaattcaaa
     5881 attaattaac agatttctag aaagactata tcttttagta taaacgtatt aatttcacag
     5941 agacaagtaa tctgtgtttt actaatatac tttacataca aaaaactctt tactttaaaa
     6001 tgaactaagc tcgcgaattc aataagtata atgaataata ttagaattca tgcactagtt
     6061 tattaaaata aagagtaatt taaaatatca ttccgtgtat taaagtgaat ggaaatgatt
     6121 agttattatt tttaacagta tctttttgtt caatagcttc taacattaat ttagtcatgc
     6181 tcgctaaatc atatttagga tcaaatcccc attcgccacg tgaacagctt gtatcaatag
     6241 aatccggcca actattagcg ataccttgtc taataggatc aacatcgtaa tctaatgtaa
     6301 aattgggata gtattcttga attgcttctt ttaccatctc tggatcaaaa ctcattgcgc
     6361 tcaaattata accatttcta gtttctaatt tagcgtcgtc tgcttccata agtttaataa
     6421 ttgcttcaat tgcatcatcc atatacatca tatccatata cgtgccttta tctatgaagc
     6481 ttgtataatg accctctctt actgctttga agtatatttc aacagcatag tctgtagtac
     6541 cgccacctgg ctctttaaca tgcgagatta aacctgggaa tctaacactt cttgtatcta
     6601 caccaaaacg tttgaaatag tattgacaca ataattctcc agctacttta tttacaccat
     6661 acattgtcgt aggttgctga atcgttactt gtggcgtatt aactttagga gttgagtctc
     6721 caaatgcacc aattgaactt ggtgtgaaaa agtgcaaatt ataagttctt gcagcttcta
     6781 atgcattcat taatccaccc atatttaaat cccaagctag aattggattt ttctcagcag
     6841 ttgctgataa taatgctgcc atatgcatta gactatccgc ttcaaagtcc ctaactaact
     6901 caaacatacg gtcacgatct gttacgtcta agatttcaaa tggtccattt tgtacaggtg
     6961 agtctgcttc aggttcccta atatctgtag caagaacatt atctgtccca taaatttctc
     7021 tgcacttaac aactaattct gtaccaattt gtcctaatgc accagtaatc ataatttttt
     7081 tcatagaaat atctcctttg tctcttctat atagctatag tccatcacaa gcggacataa
     7141 tattcatttt cataataatt ataatataaa agcgctttct tgtatatatg acatgtacat
     7201 gttgctgata tttctgtaaa tggaaattct agttgtatta attgatttta gtaatttata
     7261 gcgtttatta ttgctaatta ctgtcaaatt aaatttttta tccctcaact cttaaactct
     7321 ggatatcttt cattatatta gcttttttat aaccatggat atcatgtaaa gccttataag
     7381 ctttaaataa tgtttcatac ctttgtactt tttccgcttc tggattaatt tcgtataaaa
     7441 ttggctcttt catagcatca actgcgtcat taattgaatt atgtgcgcca ccactgactg
     7501 cacctaatat tgctgcgcct attgctggtg catactcact atccataaca attagttttt
     7561 tattcagtac attcgcatag atatccatca taacagcatt ctttttaggt ataccgccac
     7621 atgcaaatac cttttctact tccatatgcc aatcttgata ctgttgcata atcatcttag
     7681 taccaaatgc tgtagcttct aaatatgctc tataaatatc ctcatgctta gtttgtaaag
     7741 ttaatccaaa gatacaacct gttaaattgc tatcacttaa tacacttcga tttccattat
     7801 gccaatcaag agcaatgagc ccactttcac ctggcatttg atgttttatc ttttcattca
     7861 ttaattcaaa tacagtcata tttctatttt ctgcttcatc tacatatgac tttggtgctt
     7921 gcttagcgac atactcaaac aaatcaccta ctgctgattg ccccgcttca taagcaaata
     7981 attctggaat aattgctcct tttacagaac ctgatatacc tggcacttga tgttgctttt
     8041 catttaacat aagatggcat gtgcttgttc ccatcaccat agtcatttct ttatcttttt
     8101 cagacccaat acctaataaa ctagcatggg catcaataat aaaaggactt accatagttt
     8161 cttttgataa tcctaatttc tgtgccattt tatcatccag tttccctact gcttcaccaa
     8221 tattaacaac cggtgcagat actttatctt gaattacttt tgataatttg gggtctattt
     8281 tatcaaataa atcataatga aaccctgttt cttcttccca aaatgctttg aaacctaatc
     8341 cacaattcga gcgtacattt ttattagtta atttatttac aatccaatcg cccgcttcca
     8401 taatataagc cgttttttcc ataatttctg gtgctcgatt catgacctcc attattttgg
     8461 gaatcatcca ttcactacta acattatatc cataatggcc taaccactta ttattatttt
     8521 caatagcagt ttgatataat ttctctgcct ctttatatgc accatgatgt ttccaaagtt
     8581 tcacatacgc atgtggattg tttttaaatt gttttaagtt atgtaccggg ttaaggtttt
     8641 cgtcagtaaa aataatagta gatgaagtaa agtctatacc aatacctact atattgtctg
     8701 gatctatttt tgattcacgt actatatatg atattccttc ttccataatt tctaaataat
     8761 cattactatt ttgaagtgca tatgtatgtg gtattttcaa accatttaat tcactctcaa
     8821 tgacaccatg tgtatatggt ttcacaaatt ttgatactac ttgaccgtta gttgtattaa
     8881 ttaaaaacac acggcctgaa gcagttccat aatcaattcc aatgctataa gacatcatgt
     8941 ttcctccata tcgctagttg cggttattag ttttgcaagc gttttcttaa atcatactca
     9001 taataggttc tattttacaa gtatcactta aattttgcca aaaaaaaaag agccctcata
     9061 catttacata tgaggctcct ctaattaata tttaattatt tattttgtat tgcatttaac
     9121 atttcattaa ctgctaattt tcctaactca tttgaagcta aaggactgtc acctgttaat
     9181 aatttacgat cttttaacgt tcttcctgtc atatcgtcgt taactacttt taatccttgt
     9241 ttagttaata aatcagcaac taaccatttc aagcgtccag gtaaataacc tatttcaata
     9301 tttgcacctt catctaatga gtcagggaag acacaaacag agtatccttc taatggagat
     9361 ttttctctgt taagccctgc tgaaagtagt gctgctggtc catgacataa tgtaactata
     9421 aagcggtcat tgtctaatgc ccaatctaat gtttgttgaa cgtcctcact ttcagaaata
     9481 ccaacaactg cagcatgtcc gcctgggata aagacagata aatagtctga atcaggtcct
     9541 aattcatttt taatcacatc tgctaatttt tttggctgtt ttaatttttc tttcaattta
     9601 ttataagtac ttataactgc ctcgtcttca gttggcatag cccataattc taatttaact
     9661 ggataaccag ataatgtcgc aacatcaacg tcaaaacctg cttccattaa atgatgtaaa
     9721 ggtaataaca tttcaacagg atgattaccc gtagagaaca tttttccatt ttccaataat
     9781 acatatcgct cttcagcagc aatcatcaat actttccatt taccatcttt ataggcacct
     9841 ttgtgttcaa caccatcaaa atctgtttta ggtgctgtat attgactaag ggaatatggt
     9901 gatgggaaaa atgcgttatc ttctgcttta tctggcgttg gttgcttact taattcattt
     9961 acatcttgtg acatagttta ttcctacttt ctctatatag ttactactag tatagacata
    10021 tactacacac tataaacatg tattactatt aaaatgctca ttatcaaaaa taatcaaaat
    10081 gaatttcttt gaaaaaaata caactaaatt ctacaagatg cttttaaaat tcaaacttca
    10141 attttgggga gcactaaaca ttgatatacg aacttactgt taataaacta aacattaaaa
    10201 aaagcccacg acaatatttg ccgtgggaat aaataaatat taaatcaact tcatttcttt
    10261 tcctactttt tcataagccg caattgcttc atctaacatg tcttttgtat gtgcagctgt
    10321 aggcatattt cttacacgtc ctgtacctct tggtactgtt gggaaaacga tagatttcac
    10381 atagacacct tcgtctttta aacgcttact aaattcttga gttgtttttt catcaccaat
    10441 aattactggt gtaattggag tttctgactc acctgtatca tatcctaatt ttgacaatcc
    10501 attttttaaa tattgtgcat tgttccataa tttatcatgt aattcagttg aatccattaa
    10561 ctttttaact gcttcagtta ttgctttggt atccccaggt gctaatgatg tagagaataa
    10621 gaatggtcgt gattgtgctt ttaaccaatc tattaactct tttgtacctg ctacataacc
    10681 gccaacgaca ccaattgctt tagaaagcgt acctatttgg aaatcgattt tatcttgtaa
    10741 accaaaatgt ttaaccgtac cagcgccttt acccataaca cctgaaccat gagcgtcgtc
    10801 aacataagtt aataaaccaa attcttctgc aatttctaca atttcaggta atttagccac
    10861 atcaccatcc atactaaaaa cgccatcagt gatatacatc actttattgt attgacctga
    10921 ttcaactgct tcttttgctt tcgcacgtaa atcatccatg tctgaatggt taactcgaat
    10981 aattttagct ttagataagc gacatccatc aataattgat gcatgattaa gctcatctga
    11041 taaaatagca tcatttttat tcatgacagc tgaaatagca gccatattac aattaaatcc
    11101 tgattgataa gctattgcag cttctgttcc tttaaatttt gctagtgttt cttctaattc
    11161 gtcgtgtaaa tctaatgtac cattgattgt acgaacagcg cctgcaccta caccatgtgt
    11221 atcaatagct gcttttgcag ctgatttcaa atcttcattt gttgctagtc ctaaataatt
    11281 atttgaagat aagttaatgt atgatttccc attgattttg atttctggtc cgtttgcacc
    11341 ttcaattgta tctatttcat tatacaaacc attttctttt agataattta tattttcctc
    11401 taaaaactca tgtaatgatt gaaccacagt atataatccc ccttaagtat tgtttggtaa
    11461 tacctttatc atacagtaat tctacataac ttgaaagcct tatcattcat agataaaata
    11521 tctcaaagca atcatttagt acgataattt cataaaatgt tatactataa acattatttt
    11581 agaaagtagt gataagggtg ttagattggt tccaactagc aaataataaa gaaaataaaa
    11641 caattcaact cagacgttat ttacaccaat atcctgagtt atcttttgaa gaatttcaaa
    11701 cacatgatta tattgttaac caattaagcc agttatcttg tgatattgaa actcctatcg
    11761 gccgcaatgg tatcaaagcc acatttaaag gcttgggcac aggacctacc attgcattaa
    11821 gagctgactt tgatgcgtta cctgtcgagg aattaaacga tgtgccttat aagtctaaaa
    11881 atccaggatg tatgcatgcg tgtgggcatg atggtcatac agcaatttta cttactgtag
    11941 cagagatttt agatgaacat aaacatttat tagaaggtaa tgttgtatta atattccagt
    12001 acggtgaaga aatcatgcca ggtggttctc aagaaatgat agatgctgga tgtctggaaa
    12061 atgtagatag aatttacggt acacatttat ggagtggtta tcctactgga actattcatt
    12121 cacgtgcggg ggcaatcatg gcctctccag atgaatttag cgtcacattt aaaggtcgtg
    12181 gtggtcatgg tgccaaacct catgaaacca ttgacccaat agtaatcatg gcagagttca
    12241 ttttaagtgc acaaaaaata atttcacgta cgatcgatcc agttaaacaa gctgtacttt
    12301 catttggaat gattcaagca ggcaccacag atagcgttat tcctgatcaa gcattttgta
    12361 aaggtacagt tagaactttc gattcagata tacagaatca tgtaatggac aaaatggata
    12421 agcttctaca aggtttagca atagcaaatg atattaacta tgatttgaat tatattaaag
    12481 ggtatttgcc tgtccataat aacgagaaag catatcaagt tattaaagaa gcaacaaatg
    12541 acttacatgt acgctttaat gaatcagatt taatgatgat tggagaagat ttttcacatt
    12601 atttgaaagt acgaccaggt gcattctttc tgactggttg tggtaatgaa agtaaaggta
    12661 ttacagcgcc acaccataat cccaaatttg atattgatga aaaatcatta aaatacgctg
    12721 tagctgtatt tttaaaaatc atagaattag agcaagtatt taaaacaaat taagcattta
    12781 aattaattga aagatatgaa tctgagacat aaactaatga aatgctccct tcaaagtaga
    12841 cattgaaaaa atgaaaactt tgaagggagc attttacaat gtccaagact caacaatttt
    12901 atttaaacag tattatcagt tataagtttt attagtcagt ctttcatcaa ttattgttct
    12961 tctaaatcga atagccatta caaaatttac acattaaaaa aagacccctc gattgaggga
    13021 tcttcttttt atataaaatc taagaaatta gaaattattt aatgatttca gtaacaacgc
    13081 ctgatcctac agtacgtcca ccttcacgga ttgagaaacg agtaccgtct tcaatcgcga
    13141 ttggagcgat taattctact gtcatttcaa cgttatcacc aggcattacc atttcagtac
    13201 cttctggtaa gtgaacaaca ccagttacgt cagtagtacg gaaatagaat tgtggacgat
    13261 agtttgagaa gaatggagtg tgacgtccac cttcgtcttt tgataatacg tatacttctg
    13321 ctttgaattc agtatgtggt gtaattgaac caggagcagc taatacttga ccacgttgta
    13381 cgtcttcacg agcaacacca cgtaataatg caccaatgtt gtcaccagct tcagcgtagt
    13441 ctaataattt acggaacatt tcaacacctg taacagttgt tttagatgtg tcatgtaaac
    13501 cgatgatttc aacttcttca ccaactttga tttgaccacg ttcaacacgg cctgtagcaa
    13561 cagtaccacg accagtgatt gagaatacgt cctcaactgg catcatgaat ggtttgtcag
    13621 aatcacgttc tggagttgga atgtaagtat ctacagcttc cattaattct aagatttttt
    13681 cttcgtattg agcatcgcct tctaaagctt ttaatgctga accagcgatt acaggtacat
    13741 cgtcacctgg gaagtcatat tcgcttaata agtcacgaac ttccatttct actaattcta
    13801 ataattcttc atcgtcaacc atgtcaactt tgtttaagaa tactactaat gctggtacac
    13861 caacgttacg tgataaaaga atgtgttcac gagtttgtgg cattggaccg tcagcagcag
    13921 atactactaa gataccgccg tccatttgag cagcaccagt gatcatgttt ttaacgtagt
    13981 cagcgtgtcc tgggcagtca acgtgagcgt agtgacgttt gtcagtttgg tactcaatgt
    14041 gagaagtatt gattgtgata ccacgttctt tttcttctgg agcgttgtca atcatgtcat
    14101 atgattgtgc aactgagtca ccattttttg ctaatacagt agcgattgct gctgttaatg
    14161 ttgttttacc atggtcaacg tgaccgatag taccgatatt ggcatgttct ttagaacgat
    14221 cgaatttttc ttttgccatt ataaaatctc tcctattcta attaaaaata atatttaaat
    14281 ttatctctca tgatagtttc tcaccatcat gagaaattat ttatagcgat gcaaaaagaa
    14341 ttttacaatt cttttataat gcattcaccc caaaaaatca aactataaag atgatagctt
    14401 acatttaagc tcaccttgta ttttaaccta ggctagctag tcaaaacaag ttatattatt
    14461 cacctttatt tttcttgata atatcttcag cgattgattt tggaacttca gcatagtgat
    14521 cgaagtacat agtgtaagta ccgcgacctt gagtgtttga acgtaatgat gttgcataac
    14581 cgaacatttc tgaaagtggt acataagcat taacaacttg tgcattacca cgaggttcca
    14641 taccatcaac acgtccacga cgagatgtta cgtcacccat gatatcaccc atgtactctt
    14701 caggcatttc aatagttact ttcatcattg gttctaagat tacaggatca cattttttag
    14761 cagcttcttt aagtgctaat gatgcagcaa ttttgaaggc catttcagat gaatcgacat
    14821 catggtatga accatcatat aatttagctt taacatcaat taaaggataa cctgctaaaa
    14881 caccattttc catagcatct ttaagaccag cttctactga tggaatgtat tcacgaggaa
    14941 ctacaccacc aacgatagcg ttttcgaatt cgaaacctgc gcctgtttcg tttggtgtga
    15001 attcaatgtg aacatcaccg tattgaccac gaccaccaga ttgacgagag aatttacctt
    15061 gaacttgtgc agatgatttg aatgtttcac gatatgaaac cattggagca cctacgttac
    15121 attcaacgtt gaattctttc ttcatacggt ctactaagat gtctaagtga agctcaccca
    15181 taccaccgat gataacttgt ccagtttctt cgtcagtgtg tgcatggaat gttgggtctt
    15241 cttcttgtaa tttaactaaa gcttgagtca ttttatcttg gtcagcttta gattttggct
    15301 ctactgataa gtgaataact ggctctggga attccattga ttccaagata atgtcatttt
    15361 tctcaccaca taaagtatca ccagtacctg tatctttaag acctaccgca gcagcgatat
    15421 ctccagagta tacagtatcg atttcttgac gtgagttagc gtgcatttgt aataaacgac
    15481 ctacacgttc acgtttgcct ttagtagagt tcttaacgta tgaaccagat gtcattgtac
    15541 ctgaatacac acggaagaat gttaatttac caacataagg gtcagtcata actttgaacg
    15601 ctaatgcagc gaattcagct gaatcgtctg ctttcgcgat tacttcttct tcagggttgc
    15661 tagcacggtg accaataatt ggtttaacgt ctagtggtga aggtaagtaa tcaattacag
    15721 cgtcaagcat taattgaaca cctttgtttt tgaaagctgt accacaaagt actgggtaga
    15781 attctacgtt agtagtagct tggcggatag cttcttttaa ttcagaaact gaaatttctt
    15841 cgtcaccaag atatttttcc attaattcgt cgctagtttc tgcaactgct tcgattaagc
    15901 tagcacgagc ttcttcagct ctatctaagt ggtcttcggg aatttcaatt tcttcaattt
    15961 cagtacctaa atcatttgta tatttgaaac atttcatttc aactaagtca atgattgctt
    16021 cgaattcgtc ttccgcacca attggtaatt ggattggagc agcgttagct tgtaaacgat
    16081 catgtaatgt acttacagag tattcgaagt tagcacctaa tttgtccatt ttgtttacaa
    16141 atacgatacg tggaacacca taagttgtag cctgacgcca aactgtttca gtttgaggtt
    16201 caacacctga ttgtgcatca agtactgtaa ctgctccgtc aagtacacgt aatgaacgtt
    16261 caacttctac agtgaagtct acgtgtccag gtgtatcgat aatgtttaca cggtgacctt
    16321 cccaagctgc tgttgttgca gcagatgtga tagtaatacc acggtcttgt tcttgctcca
    16381 tccagtccat ttgtgaagca ccttcgtgtg tttcaccaat tttgtggata cggccagtgt
    16441 aataaagaat acgttcagtc gtagtcgttt taccagcatc aatgtgagcc atgataccga
    16501 tattacgagt tttttctaat gaaaattctc tagccatgta ttttttctcc ttccagttta
    16561 tattactgaa taaatacgat agatatggcg atgccctaag catgaacgct taatgaaaat
    16621 tcattaatat agaacacact cagggtaaaa gcttttatct taccaacggt agtgagcaaa
    16681 tgctttgttt gcttcagcca ttttgtgagt gtcctcacgt ttcttaacgg caccacctgt
    16741 attatttgct gcatctaaaa tttcgttagc taaacgatct tccatcgttt tttcaccacg
    16801 aagacgtgca tagttaacta accaacgtaa acctaaagta gtacgacgct ctggacgaac
    16861 ttctactggt acttgatagt tagaaccacc tacgcgacga gctttaactt ctaatactgg
    16921 cataatgttg ttgattgctt cttcgaatac ttctaatgca tcacgaccac tgcgttgttc
    16981 aactaggtcg aatgctgaat aaagaattct ttgtgctgtt ccacgtttac catctaacat
    17041 aattttgtta attaatttag ttactaactt agagttatga attggatctg gtaatacgtc
    17101 tcttttaggt actgatcctt tacgaggcat aatgtaaatc ctcccttcct tatattaaat
    17161 attttaagtt taagatttaa ttaaaaacta aattcttagt ttttaggttt cttagttccg
    17221 tataatgaac gaccttgtct acgtccgtca acacctgaag tatcaagtgc tccacgtaca
    17281 atatggtaac gcacacctgg taagtctttt acacgtccac cacgtacaag tacaacactg
    17341 tgttcttgta agttatgtcc gataccaggg atgtatgcgt taatttcgat gttgtttgat
    17401 aaacgcacac gtgcatattt acgtaacgct gagttaggtt ttttaggtgt cattgtacct
    17461 acacgagtac atacaccacg tttttgtggt gagtttaagt cagtaaattt tttcttttta
    17521 ctgttgaaac ctttatttaa agctggagaa tctgattttt tgattttgct ttgtcttggt
    17581 ttacgtacta attggttaat agttggcatg tgatatgtcc tcctctcttc actttttaat
    17641 cccacacatc caggtggttc atttttaggt taaataaaat ttagtaagta aaacacttac
    17701 taattctcat ttaatcaatg ctactattgt cgcattgacg ttaataccta catgtttacc
    17761 caaagcatgt ttgcttttga aaaaagatac aggtatattt ttctgattga tttggcttaa
    17821 cacgcgagtc attaaatata cttcaacgtc ttcagcaata atcaaagatg taacttgatc
    17881 tttctttaac gctttaagcg tttctttaag accaactaca aaatgttgtt tgttaaagcg
    17941 tgcaactttt tccttagaca ataatttatc ctccaaagtt ctgcttgcat caacctttaa
    18001 tatattaaca ttgaacaaga gaattcgtca acattaaatc aaataattta cataaaaata
    18061 acaagaggct aaagcattag cctctgttat atacttgtta ttccgttact tcagtttgag
    18121 attcaacttc tgcaactggt ttagctgttt tttcgtattt tacgtcgcta taacgtctca
    18181 taccagtacc agctggaatt aacttaccaa taattacgtt ttctttaaga cctaataagt
    18241 catcacgctt acctttaatt gctgcatctg taagaactct tgttgtttct tggaatgatg
    18301 ctgcagataa gaaactttct gtttcaagtg atgctttagt aataccaagt aatactggtt
    18361 tagctgttgc aggacgctta cggtgtttaa atgcttctct atttgcatct gtaaagttat
    18421 gaatatcaac taatgaacct ggtaataact tcgtatcacc tgcttcgata attctaactt
    18481 tacgtaacat ttgtcgaacc ataacctcaa cgtgtttatc gtcgatttct acaccttgca
    18541 tacggtaaac tttttgtact tcttttaata agtagctttc agtcgcgttt aatccagcaa
    18601 cagataagta attcttaggt tcaatagaac cttcagttaa tacttcacca cgttgaactg
    18661 gttgaccgat ttctacaata attcttgaag tacctgaagc aaggtatgat cttgtttcat
    18721 tagcaccttt aacaacaatt tcttgttgtc tatcttttgc taatttaata tcttctacga
    18781 caccttcgat ttccgtaatt accgcttgac ctttagggtt acgtgcttcg aaaatctctt
    18841 gaatacgagg aagaccttgt gtgatatcgc tacctgctac cccacctgta tggaatgtac
    18901 gcattgtaag ctgtgtacct ggttcaccga tagattgggc tgcaattgta ccaactgctt
    18961 caccaacttc aactttttca ccagtagcaa ggtttttacc gtaacatttt tcacaaacac
    19021 catgtcgtgc gttacaagta aatgctgagc gaatatacat ttgttcaata ccagcatctg
    19081 taattttctt agcaatttca ggtgtaatta attcatcagg acgaatgatt atttcatcag
    19141 tttcaggatg atgaattgtt tctttagaat aacgaccttc aatacgttcg ataaatggtt
    19201 caatcatttc tgtaccttct ttaatatcag aaactaataa acctctatca gtaccacagt
    19261 cttcttcacg aacaataaca tcttgtgcca cgtcaacaag acgacgagta agatatcctg
    19321 agtcagctgt tttaagtgct gtatcggcaa gacctttacg tgcaccgtga gttgagatga
    19381 agtattcaag tactgttaaa ccttcacgga atgaagatgt gattggtaat tcgataatct
    19441 taccagatgg tgcggccatc aatccacgca tacctgctaa ctgtgtaaag ttagatgcgt
    19501 taccacgggc acctgaatca ctcatcatga agattgggtt agttttatca agtgattgca
    19561 tcaattcacc ttgaatttga tcttttgcat ctgtccaaat ttcaacaact gcattatatc
    19621 tttcttcttc agtgattaaa ccacggttga attgttttgt aattctgtcg actaattttt
    19681 catgctcatc aagtatttgt tgcttatcag gtaatactac gatatcagca acacctactg
    19741 taataccagc tttagatgag aatttgaatc ctaagtcttt catacggtct aacatcattg
    19801 atgtatcagt gatgctaaat ctgttgaata cttctgcaat aatattacct aagaattttt
    19861 tgttgaaagg ttcaattaat tcttcatttt caaagtattc ttttaatcca ccttcaccta
    19921 aagttgtagg atcgatgaaa tatctgtttg gtgtctttct ttctaagttt tcttgcgtag
    19981 gttcattaat ataagcaaat gaatctggaa tgatttcatt gaatataatt ttacctactg
    20041 acgtagcaag aatctttttg ttttgttctt cagtaaatgt tggattattg aacgaacttg
    20101 catgtacacc aattctagtg tgtaaatgta caaagccatt tgcatatgct tttaatactt
    20161 catttgtatt attaaagatt gcgcctgtat ttactgcatc ttttctttct aaagtaaggt
    20221 aatagttacc aagtaccata tcttgtgatg gtgtaactac aggtttacca tctttagggt
    20281 tcaagatgtt ttgtgctgct aacatcaaca ttcttgcttc agcttgtgcc tcttttgata
    20341 aaggaacgtg aaccgccatt tggtcaccgt caaagtcagc gttataagct gttgttacaa
    20401 gtggatgtag acgaatcgca cgaccttcaa ctaaagttgg ttcaaatgct tgaataccaa
    20461 gtctatgaag tgttggtgca cggttaagta atacaggatg ttctctaatt acttcttcca
    20521 atacgtccca aacttcatca tccatacgtt cgattttact cttcgcattt ttaatgttag
    20581 ttgcaatttc acgttgaact aattctttca ttacgaatgg tttaaatagt tcaagtgcca
    20641 tttcttttgg taaaccacat tggtacattt tcaagcttgg acctactgca ataactgaac
    20701 gtcctgaata gtcaacacgt ttaccaagta agttttgacg gaaacgacct tgtttacctt
    20761 ttaacatatg agataaagat tttaatggac ggttacctgg gccagtaact ggacgaccac
    20821 gacgaccatt atcaattaaa gcgtcaacgg cttcttgtaa catacgtttt tcgttttgaa
    20881 cgatgatacc aggtgcacct aaatctaata aacgtttcaa acgattattt cgattaatta
    20941 cacgacggta taagtcgttt aagtcacttg ttgcaaatcg tccaccatct aattgaacca
    21001 ttggacgaat ttctggtggg atgattggaa gtacatctaa aatcatccat gaaggtttgt
    21061 tacctgaatt acggaatgat tcaacaactt ctaaacgttt aattgcacga gtaagtcttt
    21121 gaccagtagc tgattccaac tcatcgcgta acaatttaag ttcttcgtca agatcaatct
    21181 cttcaagtaa atctttaata ccttctgcac ccatttttgc aacgaattga cctgggtatt
    21241 tatcataata atctctgaat tcagcttcag ataataaagt tttcttttct aaaccagttg
    21301 gacctggatc tacaacaaca taagaagcaa agtaaataac ttcttctaat gctcttggtg
    21361 acatgtcaag taataatccc atacgacttg gtataccttt gaaataccaa atgtgagaaa
    21421 ctggagcagc aagttcaatg tgacccattc tttcacgacg tactttagat ttagttactt
    21481 caactccaca tctgtcacag accatgcctt tgtagcgaac acgtttgtat ttaccacaac
    21541 tacattccca gtcttttgta ggtccgaaaa ttctttcaca gaatagacca tctttttcag
    21601 gttttaatgt acggtagttg attgtttcag gttttttaac ttcaccaaaa gaccaagaac
    21661 ggattttttc aggtgaagcc aatcctattt tcatataatg gaaattattt acatcaatca
    21721 aggagcctac ctccttcaat ttagattagc tgtgctattt gattgatttg catttattta
    21781 aaatggctta aagtactaaa ctgaatcatc aataaaattc agcttagtat ctttttgcct
    21841 gttttgtaaa ttgcgtatta atcagtaact tctttttgtg tttcaggagc atcattttgt
    21901 tgtaaatcta ctttgcgttc tacaacatca tcgtcatcaa cgtctgtcat ttcgatttca
    21961 ttatcttgct catccataac ttttacatct aaacctaaac tttgtaattc tttcatcaat
    22021 actcggaatg attctggaac acttggtcta gagatgtttt cacctttaac aatagcctcg
    22081 tatgttttca cacgtcctac tgtatcatcg gatttgtaag ttaagatttc ttgtaatgtg
    22141 tatgcagcac catatgcttc aagtgcccat acctccatct caccaaaacg ttgtccaccg
    22201 aattgcgctt taccgccaag tggttgttgt gtaacaagtg aatatggtcc tgttgaacgc
    22261 gcatgtaatt tatcatcaac catgtgcgca agtttcaaca tgtacattac acctactgaa
    22321 atacggttat cgaatggttc acctgtacgt ccatcataaa gtacagtttt accatcacga
    22381 gccataccag cttcttcaat tgttgaccat acatcgtcat cgtttgcacc gtcaaatact
    22441 ggtgatgcaa cgtgaatacc aagattttta gcagccatac ctaagtgtag ctctaatact
    22501 tgtccgatgt tcatacgaga tggtacacca agaggattta acatgatatc gatcggacgt
    22561 ccatctggta agtaaggcat atcttcttca ggaacaatct tagaaatgac acctttgtta
    22621 ccatgtcgac cacacatctt atcaccaaca tgaattttac gtttttgaac gatatataca
    22681 cgtactaatt ggtttacacc aggtgataat gtatcgtcgc cttcttcacg attgaatact
    22741 tttacatcaa gaacgatacc gccagcgccg tgaggtacac gtaatgaagt atctctaact
    22801 tcacgtgctt tttcaccaaa gattgcatgt aacaatcttt cttcggcagt taactcagtt
    22861 acacctttag gcgttacttt accaactaaa atatctccat cttttacttc tgcaccaata
    22921 taaacgatac cacgatcgtc taagttctta agtgcacttt cagaaacatt aggaatatct
    22981 cttgtgattt cttcaggtcc taacttagta tcacgtgctt ctgattcata ctcttcaata
    23041 tgaatagaag tatacacgtc atctttcaca agtctttcac tcatgataac ggcatcctca
    23101 tagttgtaac cgtcccaagt catgaaacca actactacgt ttctacctaa tgccatttct
    23161 cctaattcca tagatggtcc atctgctaaa atctcgttat actcaacaac atctccaact
    23221 gcaacgattg gacgttggtt gtaacatgta cctgagtttg aacgtttaaa tttagctaat
    23281 ggatagcgat ctaattcacc ttcatgctca acgccgttct cttcaactag acgacgaaca
    23341 agaatttcat tagattcaac atgttcaaca cgacctctgt gcttagctgt aatagccgca
    23401 ccagaatcac gtgctgcaac gtgttccata cctgtaccaa caaatggtgc ttctggattc
    23461 atcaaaggca ctgcttgacg ttgcatgttc gcacccatca atgcacggtt tgagtcatca
    23521 ttttctaaga atggaataca tgctgtcgct gctgaaacaa cttgcttcgg cgatacatcc
    23581 atataatcca ttttttcttt agccataact gtattgttac cacggaaacg acatacaact
    23641 tcatcatcca tgaaacgacc attttcatct aatttagagt ttgcttgtgc tacaacatag
    23701 ctatcttctt cgtcagctgt taaatagtca atttgatcag tgatagcatg tgtatctaaa
    23761 tcaactttac gatatggtgt ttcaataaag ccgaattcat ttacacgtgc ataacttgat
    23821 aatgagttaa tcaatccaat gtttggtccc tcaggtgttt caattggaca catacggcca
    23881 tagtgagagt agtgaacgtc acgtacttcc atttgagcac gttcacgtgt taaaccacca
    23941 ggtcctaatg ctgatagacg acgtttatgc gttaactcag ctaatgggtt tgcttggtcc
    24001 atgaattgtg ataattgaga gctaccaaag aattctttaa tagatgcaat aacaggtcga
    24061 atattaatta attgttgagg tgtgatagac tcagtatctt gaattgacat tctttcacgt
    24121 acaactcttt ccattcttga taaaccgata cggaattggt tttgtagtaa ttcacctaca
    24181 gaacgtaaac gacggttacc taaatggtca atatcatctg tatatccaat accgcttaat
    24241 aagttaaaga agtaactcat tgaagcaatg atatctgctg gtgtaatgca tttaacttct
    24301 gagtcaggga aagcattacc aattacagtt gtcgtacgac cttcatcatc gttaggaaca
    24361 tatactttaa ttgattgaat ttctactggc tcgtctataa cgctaccatg caattcaaac
    24421 acttcgctgt ttgcatttga ttcaagtaca tccatgattt cgtcgatttt acgacgatca
    24481 agcactgtac cttcttcaac tacaatttca ccagtttcag tatttacaat tggctcagct
    24541 aatttttgat taaataaacg atgttttaaa tgtaattttt tgtttgtttt ataacgaccc
    24601 acgcttgcta agtcatagcg ttttggatca aagaaacgtg aatacaatag acttttagca
    24661 ttttcaacag ttggtggttc acctggacgt aaacgttcat agatttctaa taacgcttgt
    24721 tcagtgtttt cagtgccgtc tttctctaaa gtattacgta aatattcatt gtcacctaaa
    24781 aggtcaacaa tttcttggtc gcttgagaaa cctaatgcac gtaacaatac tgttaatggt
    24841 agtttacgtg ttctatcaat acgtacgtat acaacatctt tagcatctgt ttcatattct
    24901 aaccatgcac cacggtttgg aataattgtt gcatcatagt tttcacgacc atttttgtcg
    24961 attttttcat tgaaataaac ggatggtgaa cgaactaatt gagatacgat tacacgttct
    25021 gcaccattga taacgaacgt acctgtatca gtcattaatg ggaaatcacc cataaagact
    25081 tcttgttctt taacttctcc tgtttcttta atgattagac gcacttttac acgaagaggt
    25141 gcagcataag tagcgtcacg gtttttagat tcttctaaat catattttgg ttctcctaaa
    25201 cggtaatcca caaactctaa tgacaaatta ccagtaaaat cttcaattgg agaaatgtct
    25261 ctaaacattt cgattaaacc ttctcttagg aaccactcgt aagatttagt ttgaatttct
    25321 attaagtttg gtaattctaa tacttctgaa attctcgcgt agtttctacg tttacgatgt
    25381 cttccatatt ggacaacttg acctgccaaa cagattcacc cctcaaaaat tatgtgatcc
    25441 gcttttagct aaataccata agcatattaa taaaaagaca aaaagaaaac ggcaacacta
    25501 atgatgacac cattttcgat tctatattta ttcttaatta acttatctat ttaacgtaaa
    25561 agtacacact tattgaacat aaatttttac attatataac tataacagaa tatccatttc
    25621 atttcaagct tttatacttc tcagaatgta atatccttta tctttattta ccacttctac
    25681 atttccaaaa agttcattca ttcttttctt tgcagatggc ataccttgct tcttctgaat
    25741 tacaacgaat agttcaccgt tcgagtctaa tctatgtaat gcttgctcga atatacgatg
    25801 cacggtttct ttccctgctc ttattggtgg attggttaaa ataaaatcaa aacttttgtc
    25861 ttccacagca gacaaagcat cactttcctt tacgatcaca ttatcaatac catttaattt
    25921 tttgtttttt tcaactaagg ctagcgctct gtgattaaca tctagcattg taattgaatg
    25981 atgtggtgat actttagcaa tcatcaaacc aattggtccg taaccacaac caacatcggc
    26041 aattcgctta cttggaccag gtggatgcgc ttttaaaaaa gtttgaacaa gaacatctga
    26101 accataatct actttatctt tcgaaaacac tccgttatca gttattaaat caattttatg
    26161 atggtttaat tgatattgaa tacgttgttc attgctaatt acacttggat cttcatcgta
    26221 ataatgactc attattccac ctcatcttca ctttgtaact cgtcattata attataacat
    26281 ttcactcagt ctttaaatga ataaaacccc gttatcaaaa taacggggtt ttaacttctc
    26341 aaagaatcat tatattatag caatacttgc tataaaactt tgattattaa gtttaagata
    26401 cttgaattat tttaattcta cagtagctcc aacttcttct aattgttctt taagtttttc
    26461 agcttcttct ttaggtaaag cttctttgat tactttagga gctccgtcta ctaattcttt
    26521 agcatctttt aatcctaaac cagttgcttc tttaacagct ttaacaactt tgattttaga
    26581 tgaaccagct gaagttaact caacgtcaaa ttcagttttt tctgctgcag cgtcagcgcc
    26641 accagctgca cctgctactg ctactggagc agctgcagtt acaccaaatt cttcttcaat
    26701 tgcttttact aagtcgttta attctaatac tgacatttct ttaatcgctt caatgatttg
    26761 ttcatgatta gccattttat aattcctcca ttatttttaa tttttttacg ctaaaaatta
    26821 ttcagcgttt tcttcttttt gttctccaat agctttaacc gcataagcga agttgcgtac
    26881 aggagcttgt aatactgata aaagcataga tacaagacca tcgtgtgaag gtaatgaacc
    26941 aacagtttta acttcttctg ctgtaataac attgccttcc ataacgcctg atttaatttc
    27001 taatgcttca tgatctttag caaatccaga aattactttc gctgcagcta cagcatcttc
    27061 acttgaagtt gcaatagcag taggacctgt taagaattca tctaagcctt cgataccagc
    27121 tttttcagct gcacgacgta ccatagtgtt tttgtatact ttatactcaa caccagcttc
    27181 acgtaattgt gaacgtaagt cagtaacttc agctactgtt aatccacggt agtcaacgat
    27241 tactgttgaa actgaatttg atagtacctc agcaatttca tcaactagtt gttttttagc
    27301 ttcaatgata gcagacattc agacacctcc atttaaattt ttggtgcttt ttttaaaatt
    27361 aataaaaaaa gcactttcta cccacggcaa aaagtgcttg aaagatcata gatcatcacg
    27421 ttcaagtcaa ttttagcctc ggcaggataa attttaagtt ataaataact cctactgtct
    27481 taggtaaaat aatcacaata agtaatataa cttggaaatt acttattgtc aatatatttt
    27541 tacagactga aagatatttc tttcagcctg taattgttta tatcatttat tatttgaaac
    27601 ttgcagtatc aattttaact ccaggaccca ttgttgtagt tacagcaaca gatttgaagt
    27661 atgtaccttt agcagatgat ggtttagctt tagctaatac atcttgtaaa gtattgaagt
    27721 tttcaattaa ttgttcatca gtaaatgaaa ctttaccaat tgatgcatgt acgataccag
    27781 ctttttcagc acgatattct actttaccag ctttgatttc ttcaacagct tttttaacat
    27841 ccattgttac agttccagtt ttagggtttg gcattaaacc ttttggtcct aatacacgac
    27901 ctaatttacc aacttcaccc atcatgtctg gtgtagcaac tactacatcg aagtcgaacc
    27961 aaccttgttg gattttttga acgtattctg cttcacctac atagtcagca cctgctgctt
    28021 cagcttcagc aattttgtca cctttagcga atactaatac actttgtgat ttaccagttc
    28081 cgtttggtaa tacaactgca ccacggattt gttggtcatt tttacgtgta tcaattccta
    28141 aacggaatgc aacttcaaca gaagcgtcaa agttagcaat gcttgtttct ttagctaatt
    28201 taattgcttc ttcaacactg tagtgctgag tacggtcaac tttactagct gcttcttgat
    28261 actttttacc ttttttagcc atttatagtt cctcctttag tggttttagc ggaatttcct
    28321 cccacattac ttgctatatg caagttaaga gcagacaaca gaagaattaa tcttatgaat
    28381 aaatcaatca ttaatctagt cattcagatt atatttataa ttcatcatta ttatctctgt
    28441 tatctgcttc atcgtttaaa tcatgtaatc aaatttaata ttcgtaaaat tattctacaa
    28501 cgatacccat actacgtgca gtaccttcga taatacgcat agctgcttct tcgtcagcag
    28561 cgtttaagtc ttgcattttg ctgttagcaa tttcgcgtac ttgatcttta gttactgtag
    28621 caactttagt tttgtttggt tcgcctgaac ctttttcaat accagctgct tttttaagta
    28681 atactggagc cggtggagtt tttgtaataa atgtaaatga acgatcttca taaacactga
    28741 tttctaccgg aataattaaa cctgcttgat cttgagtacg tgcattgaac tctttacaga
    28801 atcccatgat gttcacacct gcttgaccta atgctggacc aactggtggt gctggattcg
    28861 ctttacctgc aggaatttgt aatttaacaa ctttatctac ttttttagcc acgatgtgca
    28921 cctccttgat atcgtgatgt ggtcacaggg ctcagttttg ccctcccact cttaacattt
    28981 cgtgacgaaa tgggcgctct aaaagcgcga ccacataatt ttaacacttt tctaattaaa
    29041 aaatcaatac tcttttttat cttttggtta gtttaataac tttaattgtt aattaaagct
    29101 tttctatttg atcgaattca acttctactg gtgtttctcg gccaaacata tctactaata
    29161 ctgttagctt aaacttatct gtttcaattt cttgaacttc accaacttga ttcgcaaatg
    29221 gacctgattt aatacgaact tgctcgccaa cttcgagttc aacatcgata gtcttttctt
    29281 taagacccat ttgttttaag atgaagcgaa cttcttctgg taacaatgga tttggcttag
    29341 accctgcacc tgcagaacct acaaaaccag taacgcctgg tgtatttctt accacatacc
    29401 atgattcatc tgtcatgatt aattccacta aaacatatcc agggaatgtt tttttaacag
    29461 tcgttttagc tttgccatct tttacttgag tttcttcttc ttccggtatg actactctaa
    29521 agatttgttc agtcatattc atagattcta ctcttttttc taaattcttt ttaactttat
    29581 tttcatatcc agaatatgta tgcactgcat accaacgctt tgcgccaact tcttcagaca
    29641 tgtcttcact cctctaacga aataataaat ttttcaatgc tgtaattcct aaatctaagg
    29701 catagaaaaa gactaagaag aatataacag tagaaactac aattacagta tatttaaata
    29761 gctcttcttt cgtcggccaa cttgtttttt ccatttcaga cttaacgcct ttaaagaaac
    29821 tttctttttt agccatctgc tcaacctcca aatcctttga acatattatc aaagtattat
    29881 ttcgaatgta tttatttcga ttctttatga attgtgtgcg cgttacattt tggacaatat
    29941 ttctttaagg ttaatcttgt tgccgagcct tcttgcttag gaacattata atttctattg
    30001 ccacaagctt cacaatttaa aggtattttt ctcacaataa ttcaccttta ttcattataa
    30061 taccagtata ctatacataa aatttataac aaatgtcaaa ccatcagtaa gtaacacgtt
    30121 ataattcaat tcacgtccta aggcgctttt caaatcattt tgaaataacg ttttatttta
    30181 tttttacatc gttgtatggc attataaatc actttctctt ttacatgcat caattgagca
    30241 atttctctcg gcttatattg ttcacacatt aaatacatga catctttttc aaatgcactc
    30301 aaatacttaa actgacaaat caactcttta attgctaatt tattcaaata attgttttca
    30361 acatcaaaac gctttattgc acatggatac gtaactctat attcattaat caaattatcc
    30421 attcttttat ttgtagctaa atattttctc aaataatcat actttacaga cgtaattaaa
    30481 cattgaacat aatttgtgaa aggctgctct ccactaaaat caaatgtttg gaccgaccta
    30541 tacatccgta taagtatttc ttgatataag tcttctaaat cataatgatt aaatccaaat
    30601 gttttgatgc gtcgaataat taatggttgt agatccatta ctaacttttc gaagtcttta
    30661 tcatttattg cattgttacg tttgattgta ctgtcttgag ttgtcaaatc gtatttcaaa
    30721 tattaaacta accccttcta tctaaaattt aaggttagtt taatattgtt acattcaaaa
    30781 tttcaagatg acggaaatgt catttcttat gatgtcctct tcgtattttt tcaaattctg
    30841 caaggatttc agaagataac ggaattcgag ttcttggctt gttttcactt atatcatcta
    30901 atgatttact cacatcaatt tcattttctt ttaaatctct ccacatttcg cgagatgata
    30961 ttctatatgc acctgatcca aagatagcat gttgctcact catatcactt gttacaactg
    31021 taatatgctt agtatgcttg tcataaagtt cataaacata acgttctatg aaactatcag
    31081 ctgtttcttt ttccttggta aaaatcgttt taacgccatg atacatgtat tctctttcaa
    31141 cacccgattg gtcataagca tcgaaaacac aaataatttc atctgaaata actgcattat
    31201 aatttgcaat tgcatctatt aattgcattc tagcttcttc taaattctct tttgcaatgg
    31261 cgcttagcgt tggtgattgt cctatcatat tgtatccatc aatgattaag taacgttctt
    31321 tcattatatt tctccaacat catgtctttt tcgaaatact tcgtacatca ttaaacttgc
    31381 tgcaaccgaa gcattcaaac tgtttacatg tccaaccatt ggaatcttaa tataaaaatc
    31441 gcatttatca cttactaggc gactcatacc ctgtccttcg ctaccaatta caatagccaa
    31501 tgacatgtcc gcttctagat ttctataatc tgttgcatta ttagcttcag tgccagctac
    31561 ccaaaagcca ttatctttta gttcatcgat agttttagct aaatttgtca ctcgaataac
    31621 tggtacatgt tcaattgcac ctgttgaggc ttttgcaact gtttgcgtta gtgtaactga
    31681 acgacgttta ggaataataa caccatcaac tcccgttgca tcggctgttc ttaaaattga
    31741 tcccaagtta tgtgggtctt ctaagccgtc taatataagt actgtcaata aaccttcttt
    31801 ttctttttgc tgttttaaaa attgatcgaa gtcagcatat tcatatggtg caataagcgc
    31861 tgcaacaccc tgatgtggtg catttgctaa aaaatctaat ttagattttg gtacagtttg
    31921 aacaatgatt ttttgatctt ttgcattttt taaaatttca ttaatttgtt gctttttaat
    31981 accttcttga atcaatatct tatttatcgg atgcccagta ataatcgctt ctctaacagc
    32041 atgcctacca acaataaccg tatcttccac atcgtcacta cctttcgttt actattgtta
    32101 ttattttatt taataatgcc tctaatcgtt cttctctttt ttctaaataa agaaaaccta
    32161 tcactgcttc tatcgctgaa cttttacgat atgtttgaac atcagtgttt ttagctttag
    32221 tatgactttt cgcgttacgc cctcgcttca aaatatccat ttcttcgtct gtaaaccatt
    32281 cttgctccat taaatattct aacgtttgcg cctgactttt ggcagataca tattttttag
    32341 acatttgatg tagtttatta ggcttacttt taagctttaa aacgatatag gtacgtacat
    32401 attgatctaa gactgcgtct cccatatatg ctaaggtcaa tggattcaat aatttaatgt
    32461 gattatcttg ttgattatcc acgtttaaat ctaacccctt gaggtgtgtc ttctaatata
    32521 atgttttgtg atttcagcat gtctcgaatt tcatctgctc gtgcaaaatc tttgtttttc
    32581 cttgcttcat tacgctcttc gattaatttt tcaacatctt catccaataa ttcatctgca
    32641 tttttagatt ttaacggtac acctaaaaca tcgctgaaaa tttgataaac tgctttaaat
    32701 ttatcaatta cttctgttga tgttgtgttc tctagtacat atttattcgc aagttttgct
    32761 aaatcatacc aagctgtaat tgcattagct gtattaaaat catcattcat aactgtttca
    32821 aaacgattta aaatcgcatc aatttgatca atatatgtct gttgattttc aatattagta
    32881 gcaatttgtg cgcgctcttc aattaattga taactattgc gaatacgctc tagtccacta
    32941 cgtgctgatt ctaccaattc tagattatag ttaattgggc ttctataatg tacgctaatc
    33001 ataaagaatc ttagtacatc tggatcaact tctttaataa tatcgtgaac taaaataaag
    33061 ttgcctagtg atttactcat tttttcatta tcaatattaa tgaaaccatt atgcatccaa
    33121 taattagcaa atggcgcatg attatgtgct tctgattgtg ctatttcatt ttcatgatgt
    33181 ggaaattgta aatctgaacc acccgcatgt atatcaattg taggtcctag ctcatgaaat
    33241 gccattacag aacattctat atgccatcct ggtctacctt caccaaatgg gctatcccaa
    33301 ctaatctcgc caggcttcgc ttttttccac aatgtaaaat caagtgcatc ttctttatgc
    33361 tctcctgcat ctatacgagc acccactttt aagtcatcta tggattgatg acttaattta
    33421 ccataacctt caaatttacg tgttctaaag taaacatcgc caccactttc atatgcataa
    33481 ccttgatcca ccaaatcttt aataaattga ataatgtcat ccatatggtc cattaccctt
    33541 ggatttgaag tcgcttttct aacatttaac gcaccaacat cttcatgaaa agcagcgata
    33601 tatttttctg caatttcggg aacagactga tttaattctt gagaacgttt aattaattta
    33661 tcatctacgt ctgtaaaatt tgatacatat tctacattat atccttggta ttcaaagtaa
    33721 cgtctcacta cgtcataatt aattgctggt cttgcgttac caatatgaat gtagttatat
    33781 acagtaggac cacatacata catttttact ttccctggtt ctataggctt gaacacttct
    33841 ttttgacgtg taagcgtatt atataatgta atcatcttga atctctccat tcctagtctt
    33901 ttcaagttgt cgttctaaat gcttaatttg ttcataaatt ggatcaggta gatggcgatg
    33961 atcaaatgtt tttccaactc gaacaccatc ttgcttaaca atatgtcctg gtataccaac
    34021 aaccgttgaa taacttggaa ctgattgtaa aacaactgaa tttgcaccaa tatttacatt
    34081 tgaatttatt ttaatatttc ctaaaacttt cgcaccggct gctattaaaa cattgtctcc
    34141 tatatctggg tgtcttttcc ctctttcttt ccctgtccca ccaagtgtca cgccttgata
    34201 gattgtcaca ttatcaccaa ttgtacatgt ttctcctatt acaacgccca taccatgatc
    34261 tataaataga cgctttccaa ttttagcacc tggatggatt tctatacctg tgaaaaatct
    34321 tgaaatttga gatatcgcgc gtgctgcaac atattttttt tggttgtata acttatgtgc
    34381 aatcaaatga ctccaaactg catgtaaacc tgcatacgtt gtaatgactt ctaatgttga
    34441 acgtgccgct ggatcctgct caaataccat ttttatatcg tctctcattc tttttaacaa
    34501 gatcatttcc tcctcaatga ttgaactacg taaatacata attgaagtac ctgcgaaatt
    34561 aaatatcaaa aaagcaccac taacatacaa gttgtattgt tagaggcgct tccgcacggt
    34621 tccactctga atttagcgaa taacattaat aatattgcgg gcgcttccaa attatcaagg
    34681 aaactaagtc aacttaatgc tcatcactct cattatatat ttaattcatt ttacgaaggt
    34741 gcattcatta atttctacgt tgtactcaca gcaaccgtac actctctgca tcgtataaat
    34801 ttaattacta atccttcgtt ttatataaaa tactttattc atattgttgt aatttgttaa
    34861 tacaacttta tcatatgtgg ttacgttata aaattcaagt atatactacc ttgatcttgt
    34921 ctatttcatt acttatattg ttttaaacgg tttagcactt tttctttacc aagtacttca
    34981 attgtatttg gtaattcagg accatgcatt tggcctgtta cagcaacacg aataggcata
    35041 aataattgct tgccttttat tcctgtttct ttttgaactt ctttaattgt ctttttaatt
    35101 tcagccgctt caaatggttc aagtgcttct aatttactga ataagtgcgt cattaactct
    35161 ggtacttgct ctccattaat cacttgttgt tcttcttcac caagagctgg catttcttta
    35221 aagaacattt ctgataaagg tacaatttca ccggcataac tcatttcttt ttgataaagc
    35281 gcaattaatt tgcgtcccca agataaatcc tcttctgacg gcacctcagg aatcaaattt
    35341 gctttaatta aatgaggtaa tgctaattgg aatactgttt cagtatcttt ttgtttcata
    35401 tattggttat taacccatgc taatttttgc ttatcgaaaa atgctggtga ttttgacaaa
    35461 cgcttttcat caaagatttt gataaattct tctttagaaa agatttcttc ttcaccttca
    35521 ggagaccaac ctaataacgc aataaaatta aataacgctt caggtaaata acctaagtca
    35581 cgatattgct caataaattg taaaatttgc ccatcacgtt tacttaactt tttacgttct
    35641 tcattaacaa ttaatgacat atgaccaaaa cgaggtggct cccagccaaa tgcttcataa
    35701 atcataattt gtttaggcgt gtttgaaata tgatcatcac cacgaattac atctgaaatt
    35761 tgcatgtaat gatcatctat agctactgca aaattgtacg ttggaatgcc atcttttttt
    35821 acgataaccc agtcaccaat accatttgaa tcaaatgaaa tatttccttt taccatatca
    35881 tcaaatgaat acgtttggtt ttgaggtact cggaaacgaa ttgatggttg gcgtccttct
    35941 gcttcaaatt gttgacgttg ttcttcagtc aaatgcgcat gttgaccacc atagcgaggc
    36001 atttcaccac gagcgatttg cgcttcacgt tcagcttcta attcttcttc tgtcatatag
    36061 catttatatg ctttatcttc tgctagtaac tgatctatta atggttggta gatatgttga
    36121 cgttcagatt gacgatatgg tccgtagcca ttgtctttat ctacagactc atcccaatct
    36181 aatcctaacc atttaagatt atcaaattgt gatgtttctc catcttctaa attacgtttt
    36241 ttatcagtat cttcaattcg aatcacaaaa tctccgttgt aatgtttagc atacaagtaa
    36301 ttgaataatg ctgttcttgc attaccaata tgaagatacc cagttggact tggtgcatat
    36361 cttactctta tacgatcgct catttttttc actcctaaat taaatatcag attttcaagt
    36421 tagttcatat aaattgttca tttgctatct tcgaccgtca taacaaatgt ctaactcgtc
    36481 ttattgttaa aacgaaacaa tgctttttaa catgacctta aaataatttc attgtttaat
    36541 cataacataa ttccctgggt aatatgctta aattttaaat agaaagctgt tgttttttca
    36601 acactttaaa aaagctatcc ctaagaatca agactgcact acataaacat ttcaacgact
    36661 taaaaatgaa tattatgtac tgtgaaatta aacttcaaat taactattca aatacgttaa
    36721 aattgattct
//
LOCUS       DALAGD010000014        35679 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.014, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000014 DALAGD010000000
VERSION     DALAGD010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 35679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35679
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.014"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(142..1941)
                     /locus_tag="O2701_000506"
     CDS             complement(142..1941)
                     /locus_tag="O2701_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCZ4292416.1"
                     /translation="MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEK
                     TPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISFVEAVKELGDRVNVAVDIEATQS
                     NSNVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGF
                     APDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVG
                     YSGRTYTGQEPKYLNSPETPIFQKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDT
                     AGLKNVVATMGTQLSDEHITFIRKLTSNITLMFDGDFAGSEATLKTGQNLLQQGLNVF
                     VIQLPSGMDPDEYIGKYGNDAFTAFVKNDKKSFAHYKVSILKDEIAHNDLSYERYLKE
                     LSHDISLMKSSILQQKALNDVAPFFNVSPEQLANEIQFNQAPANYYPEDEYGGYIEPE
                     PIGMAQFDNLSRQEKAERAFLKHLMRDKDTFLNYYESVDKDNFTNQHFKYVFEVLHDF
                     YAENDQYNISDAVQYVNSNELRETLISLEQYNLNDEPYENEIDDYVNVINEKGQETIE
                     SLNHKLREATRIGDVELQKYYLQQIVAKNKERM"
     gene            complement(2002..2820)
                     /locus_tag="O2701_000507"
     CDS             complement(2002..2820)
                     /locus_tag="O2701_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HCZ4292417.1"
                     /translation="MEKIKIIVASDSIGETAELVARAGISQFNPKQCKNELLRYPYIE
                     SFEDVDEVIQVAKDTNAIIVYTLIKPEMKQYMSEKVAEFQLKSVDIMGPLMDLLSASV
                     EEKPYNEPGIVHRLDDAYFKKIDAIEFAVKYDDGKDPKGLPKADIVLLGISRTSKTPL
                     SQYLAHKSYKVMNVPIVPEVTPPDGLYDINPKKCIALKISEEKLNRIRKERLKQLGLG
                     DTARYATEARIQEELNYFEEIVSEIGCPVIDVSQKAIEETANDIIHYIEQNKSK"
     gene            complement(2831..3454)
                     /locus_tag="O2701_000508"
     CDS             complement(2831..3454)
                     /locus_tag="O2701_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ4292418.1"
                     /translation="MELSQRQERIIEIVKTKGPITGEQIADKLNLTRATLRPDLAILT
                     MSGFLEARPRVGYYYSGKSKGKFFNEKLRQFEVKDYMSQPVVLRENTTVYDAICTIFL
                     EDVSTLFIINEDNDFVGVCSRKDLLRASMIGADIHKVPISVNMTRMPNVTYLEESELV
                     IYAADRMIEKEIDSIPIVRKKDNQKYEVIGRISKTTIAKLLVALYKE"
     gene            3789..5180
                     /locus_tag="O2701_000509"
     CDS             3789..5180
                     /locus_tag="O2701_000509"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HCZ4292419.1"
                     /translation="MAKDMDTIVSLAKHRGFVFPGSDIYGGLSNTWDYGPLGVELKNN
                     VKKAWWQKFITQSPFNVGIDAAILMNPKVWEASGHLNNFNDPMIDNKDSKIRYRADKL
                     IEDYMQDVKGDENFIADGLSFEQMKKIIDDEGIVCPVSKTANWTEIRQFNLMFKTFQG
                     VTEDSTNEIFLRPETAQGIFVNYKNVQRSMRKKLPFGIGQIGKSFRNEITPGNFIFRT
                     REFEQMELEFFCKPGEEIEWQNYWKTFASDWLTSLNMSSENMRLRDHDEDELSHYSNA
                     TTDIEYKFPFGWGELWGIASRTDFDLRKHAEHSGEDFRYHDPETNEKYIPYCIEPSLG
                     ADRVTLAFLCDAYDEEGVEGSKDARTVLHFHPALAPYKAAILPLSKKLSGEAIKIFEQ
                     LSSKFSIDFDESQSIGKRYRRQDEIGTPYCVTFDFDSLEDNQVTVRDRDSMEQVRMPI
                     SELEAFLTEKTKF"
     gene            complement(5330..6076)
                     /gene="recO"
                     /locus_tag="O2701_000510"
     CDS             complement(5330..6076)
                     /gene="recO"
                     /locus_tag="O2701_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCZ4292420.1"
                     /translation="MRQKGIIIKAVDYGESDKIITILNEHGAKVPLMARRAKKVKTGL
                     QAQTQLFVYGLFIYNQWRGMGTLNSVDVISQHYKLQMDLYVSSYAALAAETIERSMDE
                     GDIAPYNYQLLQFVLEKIESGTSAQLMSVVVMLKCMKRFGFTASFNRCAVSGNDTQAD
                     LIGYSFKFDGAISRQEASKDVHAVILSNKTLYLLDVLQKLPIDKMNSLNIHQEIIDEM
                     SDIILMLYREYAGMFFKSQKLINQLKRLEQ"
     gene            complement(6104..7003)
                     /gene="era"
                     /locus_tag="O2701_000511"
     CDS             complement(6104..7003)
                     /gene="era"
                     /locus_tag="O2701_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCZ4292421.1"
                     /translation="MTEHKSGFVSIIGRPNVGKSTFVNRVIGHKIAIMSDKAQTTRNK
                     IQGVMTRDDAQIIFIDTPGIHKPKHKLGDYMMKVAKNTLSEIDAIMFMVNANEEIGRG
                     DEYIIEMLKNVKTPVFLVLNKIDLVHPDELMPKIEEYQSYMDFTEIVPISALEGLNVD
                     HFIDVLKTYLPEGPKYYPDDQISDHPEQFVVGEIIREKILHLTSEEIPHAIGVNVDRM
                     VKESEDRVHIEATIYVERDSQKGIVIGKGGKKLKEVGKRARRDIEMLLGSKVYLELWV
                     KVQRDWRNKVNFIRQIGYVEDQD"
     gene            complement(7004..7408)
                     /gene="cdd"
                     /locus_tag="O2701_000512"
     CDS             complement(7004..7408)
                     /gene="cdd"
                     /locus_tag="O2701_000512"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCZ4292422.1"
                     /translation="MSYQPHYFQEVRKAQQESYSPYSQFKVGAYLKTKDGRTFYGTNV
                     ENASYPLSICAERASLVSAISQGYRPGDFESITVTVDADKPSSPCGACRQVLKELCDD
                     DMPVYMTNHKGDMVMMTVAELLPFGFSGKDLE"
     gene            complement(7419..7763)
                     /locus_tag="O2701_000513"
     CDS             complement(7419..7763)
                     /locus_tag="O2701_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCZ4292423.1"
                     /translation="MKRFKYALDGLKILIQKDYKFLLHVFAMIVAIVFGLVLNINRIE
                     WIFILIAIALVLTVEALNTAIEYVVDLVTVEYHDLAKYAKDIAAFSVLIVSILAFIIG
                     LIVFLPHFIALF"
     gene            complement(7766..8233)
                     /gene="ybeY"
                     /locus_tag="O2701_000514"
     CDS             complement(7766..8233)
                     /gene="ybeY"
                     /locus_tag="O2701_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCZ4292424.1"
                     /translation="MFTIDFSDHTGLVKDAWYKQIEDLLEFAKKEEHIEDDAELSVTF
                     VDKQEIQEINRTYRDKDKVTDVISFALEEDEPEIDFSGLDIPRVLGDIIICTDVAQEQ
                     ANNYGHSFERELGFLALHGFLHLLGYDHMTEADEKEMFGRQDTILNAYGLTRD"
     gene            complement(8234..9181)
                     /locus_tag="O2701_000515"
     CDS             complement(8234..9181)
                     /locus_tag="O2701_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCZ4292425.1"
                     /translation="MPGIIQIDDMNQSQALIGNNDEHLKAIEESFDVVIHARGQEVAV
                     KGTKIENVEKAESVLINLLKVIDLGNNITIKDVEAAIKMAHNNTIQHLLDLYDEEITK
                     DAFGKTIRAKTMGQRIYVNAMKNNDLVFGIGPAGTGKTFLAVVYAAKQLRKGAVKRIV
                     LTRPAVEAGESLGFLPGDLKEKVDPYLRPLYDGLYTVLGREQTERFIERGIIEIAPLA
                     YMRGRTLEDAFVILDEAQNTTHAQMKMFLTRLGFGSKMVVTGDQTQIDLPKGVKSGLK
                     EAVSRLHNVKGISILKLDQSDVVRHPLVSKIIEHYEGEN"
     gene            complement(9485..10183)
                     /locus_tag="O2701_000516"
     CDS             complement(9485..10183)
                     /locus_tag="O2701_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HCZ4292426.1"
                     /translation="MSVGILIFVISVIISIITTMRENSHKDRQNQKPPQKTSTDNEPK
                     KGGFFEEIERTFKEISEELNEEEKKSSKRKYDDTLPPLFDELPKEEPKSKPVVEPMAP
                     KKQQETKPMTEKPITVPKAEPVEQKHRPSRQDNSDEIRRQLEKSLRDDIKTIRTDIDR
                     EKEKQIAKMEKRARDIIEDKYLSERTKRLKLKQLLNSQNVEKDLTKSAFQFDKDEVIN
                     GMIWSEILAKPKQL"
     gene            complement(10200..11189)
                     /gene="floA"
                     /locus_tag="O2701_000517"
     CDS             complement(10200..11189)
                     /gene="floA"
                     /locus_tag="O2701_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500186.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HCZ4292427.1"
                     /translation="MFSLSFIVIAVIIVVALLILFSFVPIGLWISALAAGVHVGIGTL
                     VGMRLRRVSPRKVIAPLIKAHKAGLALTTNQLESHYLAGGNVDRVVDANIAAQRADID
                     LPFERAAAIDLAGRDVLEAVQMSVNPKVIETPFIAGVAMNGIEVKAKARITVRANIAR
                     LVGGAGEETIIARVGEGIVSTIGSSKHHTEVLENPDNISKTVLSKGLDSGTAFEILSI
                     DIADVDISKNIGADLQTEQALADKNIAQAKAEERRAMAVATEQEMKARVQEMHAKVVE
                     AESEVPLAMAEALRSGNISVKDYYNLKNIEADTGMRNAINKRTDQSDDESPEH"
     gene            complement(11207..11890)
                     /locus_tag="O2701_000518"
     CDS             complement(11207..11890)
                     /locus_tag="O2701_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCZ4292428.1"
                     /translation="MTTILESTAGDTWVEQVSNIIVQPIFTLILTCLTFLGFVYQLYS
                     KKINAAGIIATLSLLILFLGFLIQGNVNMHSILIFSIGVILVVIELFVVGAVIGIIGM
                     ILITISITTLGDNLLFMLANVIVALILTIVEWVILVKIFNRKIPFLDKVILKDSTNSE
                     SGYNSHDNRSHLVGKTAQTVTDLRPAGIIFCENERIDAVSDGNFILRNKTVKILEVEG
                     TRVVVREVD"
     gene            complement(12134..12310)
                     /locus_tag="O2701_000519"
     CDS             complement(12134..12310)
                     /locus_tag="O2701_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCZ4292429.1"
                     /translation="MSKTVVRKNESLEDALRRFKRSVSKSGTIQEVRKREFYEKPSVK
                     RKKKSEAARKRKFK"
     gene            complement(12603..13949)
                     /gene="mtaB"
                     /locus_tag="O2701_000520"
     CDS             complement(12603..13949)
                     /gene="mtaB"
                     /locus_tag="O2701_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HCZ4292430.1"
                     /translation="MSTVAFHTLGCKVNHYETEAIWQLFKEANYERVDFEANADVFVI
                     NTCTVTNTGDKKSRQIIRRAIRQNPDAVICVTGCYAQTSSAEIMEIPGVDVVVGTQDR
                     HKLLGYIDEFRKERQPINGVGNIMKNRKYEELDVPYFTDRTRASLKIQEGCNNFCTFC
                     IIPWARGLMRSRDPEKVVEQATQLVNSGYKEIVLTGIHTGGYGQDLKDYNLAQLLRDL
                     ETINGLERIRISSIEASQLTDEVIDVLERSTKVVRHLHIPLQSGSDTVLKRMRRKYTM
                     DRFSERLTKLHKALPDLAVTSDVIVGFPGETEAEFQETYDFIVKHKFSELHVFPYSPR
                     IGTPAARMDDQIDEEIKNERVHKLITLSNQLGKLYASKFDQDVLEVIPEEQGDTEGTL
                     VGYADNYMKVQFEGDESLIGQIVKVKITQANYPLNEGQAIKVVDFATNKSDREVLV"
     gene            complement(13956..14708)
                     /locus_tag="O2701_000521"
     CDS             complement(13956..14708)
                     /locus_tag="O2701_000521"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCZ4292431.1"
                     /translation="MQRYFIDQNADVSQRFFITKKEDIHHITNVMRYDVGSKIILTFN
                     DQTVYLCEIADITSDKIEVDLKEKQNINTELPVDVTICSGLIKADKYEWLLQKATELG
                     ASSFIAVSMERSIVKLNEAKVAKKIERWQKIIKEAAEQSYRLVIPSIQFESNLKLICD
                     TIDNYDYILIAYEEEAKDGELSNFKQTLQQFNAQDKVLMIFGPEGGLSENEISLFSNT
                     STVVGLGPRILRAETAPLYALSAISYEKELMG"
     gene            complement(14710..15648)
                     /gene="prmA"
                     /locus_tag="O2701_000522"
     CDS             complement(14710..15648)
                     /gene="prmA"
                     /locus_tag="O2701_000522"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCZ4292432.1"
                     /translation="MNWTELSIIINHEAVELATNILENHGSNGVVIEDSDDLINQPED
                     KYGEIYALKKEDYPDKGVRLKAYFNEMTYDDKLRQQIKDELLNLDELDQHNVQFSEQI
                     IAETDWENEWKNYFHPFRASKKFTIVPSWETYAKEADEELCIELDPGMAFGTGDHPTT
                     SMCLKAIETYVLPQHSVIDVGTGSGILSIASHLIGVKRIKALDIDEMAVSVAKENFRR
                     NHCETLIEAVPGNLLKDETEKFDIVIANILAHIIDEMIEDAYNTLNEGGYFITSGIIK
                     EKYEGIQSHMERVGFKIISEQHDNGWVCLVGQKVSE"
     gene            complement(15652..16791)
                     /gene="dnaJ"
                     /locus_tag="O2701_000523"
     CDS             complement(15652..16791)
                     /gene="dnaJ"
                     /locus_tag="O2701_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCZ4292433.1"
                     /translation="MAKRDYYEVLGISKDASKDEIKKAYRKLSKKYHPDINKEEGADE
                     KFKEISEAYEVLSDDNKRASYDQFGHDGPQGFGGQGFNGSDFGGFSGFGGGGFEDIFS
                     SFFGGGRQRDPNAPQKGDDLQYTMTLTFEEAVFGTTKEISIRKDVTCETCHGDGAKPG
                     TSKKTCSYCNGAGHVAVEQNTILGRVRTEQVCPKCNGSGQEFEEACPTCHGKGTENKT
                     VKLEVKVPEGVDNEQQIRLAGEGSPGVNGGPAGDLYVVFRVKPSETFKRDGDDIYYKL
                     NVSFPQAALGDEIKIPTLNNEVMLTIPAGTQTGKQFRLKEKGIKNVHGYGYGDLYVDI
                     KVVTPTKLTDRQKELMKEFAQLNGEEINEQPSNFKDRAKRFFKGE"
     gene            complement(16927..18759)
                     /gene="dnaK"
                     /locus_tag="O2701_000524"
     CDS             complement(16927..18759)
                     /gene="dnaK"
                     /locus_tag="O2701_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCZ4292434.1"
                     /translation="MSKIIGIDLGTTNSCVTVLEGDEPKVIQNPEGSRTTPSVVAFKN
                     GETQVGEVAKRQAITNPNTVQSIKRHMGTDYKVDIEGKSYTPQEISAMILQNLKNTAE
                     SYLGEKVDKAVITVPAYFNDAERQATKDAGKIAGLEVERIINEPTAAALAYGLDKTDK
                     DEKVLVFDLGGGTFDVSILELGDGVFEVLSTAGDNKLGGDDFDQVIIDYLVAEFKKEN
                     GVDLSQDKMALQRLKDAAEKAKKDLSGVSQTQISLPFISAGENGPLHLEVNLTRSKFE
                     ELSDSLIRRTMEPTRQAMKDAGLTNSDIDEVILVGGSTRIPAVQEAVKKEIGKEPNKG
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIEILGGRMNTLIERNTTIPTSK
                     SQIYSTAVDNQPSVDVHVLQGERPMAADNKTLGRFQLTDIPPAERGKPQIEVTFDIDK
                     NGIVNVTAKDLGTNKEQRITIQSSSSLSDEEIDRMVKDAEVNAEADKKRREEVDLRNE
                     ADSLVFQVEKTLTDLGENIGEEDKKSAEEKKDALKTALEGQDIEDIKSKKEELEKVIQ
                     ELSAKVYEQAAQQQQQAQGANAGQNNDSTVEDAEFKEVKDDDKK"
     gene            complement(18828..19454)
                     /gene="grpE"
                     /locus_tag="O2701_000525"
     CDS             complement(18828..19454)
                     /gene="grpE"
                     /locus_tag="O2701_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCZ4292435.1"
                     /translation="MTNKDESVEKNTESTVEETNVKQNIDDSVEQAEESKGHLQDEAI
                     EETSDENVIEEIDPKDQKINELQQLADENEEKYLRLYAEFENYKRRIQKENEINKTYQ
                     AQRVLTDILPAIDNIERALQIEGDDETFKSLQKGVQMVHESLINALKDNGLEVIKTEG
                     EAFDPNIHQAVVQDDNPDFESGEITQELQKGYKLKDRVLRPSMVKVNQ"
     gene            complement(19486..20463)
                     /gene="hrcA"
                     /locus_tag="O2701_000526"
     CDS             complement(19486..20463)
                     /gene="hrcA"
                     /locus_tag="O2701_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HCZ4292436.1"
                     /translation="MITDRQLSILNAIVEDYVDFGQPVGSKTLIERHNLNVSPATIRN
                     EMKQLEDLNYIEKTHSSSGRSPSQLGFRYYVNRLLEQTSHQKTNKLRRLNQLLVENQY
                     DVSSALTYFADELSNISQYTTLVVHPNHKQDIINNVHLIRANPNLVIMVIVFSSGHVE
                     HVHLASDIPFNNDKLNTISNFVTNKLTEFNQNLQDDIVSFVQSEQEEIFINKLLNTMN
                     NHISNQSNSIYMGGKVKLIDALNESNVSSIQPILQYIESNRIAELLQDISSPNINVKI
                     GNEIDDSLSDISIVTSQYHFDETLKGQIAVIGPTAMHYQNVIQLLNRIW"
     gene            complement(20563..21687)
                     /locus_tag="O2701_000527"
     CDS             complement(20563..21687)
                     /locus_tag="O2701_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCZ4292437.1"
                     /translation="MTVQSAYIHIPFCVRICTYCDFNKYFIQNQPVDEYLDALITEMS
                     TAKYRILKTMYVGGGTPTALSINQLERLLKAIRDTFTITGEYTFEANPDELTKEKVQL
                     LEKYGVKRISMGVQTFKPELLSVLGRTHNTEDIYTSVLNAKNTGIKSISLDLMYHLPK
                     QTIEDFEQSLDLALDMDIQHISSYGLILEPKTQFYNMYRKGLLKLPNEDLGADMYQLL
                     MSKIEQSPFHQYEISNFALDGHESEHNKVYWFNEEYYGFGAGASGYVDGVRYTNINPV
                     NHYIKAINKESKAILVSNKPSLTERMEEEMFLGLRLNEGVSSSRFKKKFDQSIESVFG
                     QTINNLKEKELIVEKNDVIALTNRGKVIGNEVFEAFLIND"
     gene            complement(22250..24073)
                     /gene="lepA"
                     /locus_tag="O2701_000528"
     CDS             complement(22250..24073)
                     /gene="lepA"
                     /locus_tag="O2701_000528"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCZ4292438.1"
                     /translation="MDNEQRLKRRENIRNFSIIAHIDHGKSTLADRILENTKSVETRD
                     MQDQLLDSMDLERERGITIKLNAVRLKYEAKDGNTYTFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNELELLPVINKIDLPAAEPERVKQEIEDMI
                     GLDQDDVVLASAKSNIGIEEILEKIVEVVPAPDGDPEAPLKALIFDSEYDPYRGVISS
                     IRIVDGVVKAGDKIRMMATGKEFEVTEVGINTPKQLPVDELTVGDVGYIIASIKNVDD
                     SRVGDTITLASRPASEPLQGYKKMNPMVYCGLFPIDNKNYNDLREALEKLQLNDASLE
                     FEPESSQALGFGYRTGFLGMLHMEIIQERIEREFGIELIATAPSVIYQCVLRDGSEVT
                     VDNPAQMPDRDKIDKIFEPYVRATMMVPNDYVGAVMELCQRKRGQFINMDYLDDIRVN
                     IVYELPLAEVVFDFFDQLKSNTKGYASFDYEFIENKESNLVKMDILLNGDKVDALSFI
                     VHRDFAYERGKALVEKLKTLIPRQQFEVPVQAAIGQKIVARTNIKSMGKNVLAKCYGG
                     DISRKRKLLEKQKAGKAKMKAVGNVEIPQDAFLAVLKMDDE"
     gene            24419..24670
                     /locus_tag="O2701_000529"
     CDS             24419..24670
                     /locus_tag="O2701_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCZ4292439.1"
                     /translation="MANIKSAIKRVKTTEKAEARNISQKSAMRTAVKNAKTAVSNNAD
                     NKNELVSLAVKLVDKAAQSNLIHSNKADRIKSQLMTANK"
     gene            complement(24715..25689)
                     /gene="holA"
                     /locus_tag="O2701_000530"
     CDS             complement(24715..25689)
                     /gene="holA"
                     /locus_tag="O2701_000530"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCZ4292440.1"
                     /translation="MSDNIVAIYGDVPELVEKQSAEIISQFLKSDRDDFNFVKYNLYE
                     TEIAPIVEETLTLPFFSDKKAILVKNAYIFTGEKAPKDMAHNVDQLIEFIEKYDGENL
                     IVFEIYQNKLDERKKLTKTLKKHARLKKIEQMSEEEIKKWIQSKLNENFKDIKRDALD
                     LFIELTGINFNIVSQEIEKLILFLGDRPTINKQDVNQIINRSLEQNVFLLTEYIQKRK
                     KEQAIHLVKDLITMKEEPIKLLALITSNYRLFYQCKILSQKGYSGQQIAKTIGVHPYR
                     VKLALGQVRHYQLDELLNIIDACAETDYKLKSSYMDKQLILELFILSL"
     gene            complement(25746..27947)
                     /locus_tag="O2701_000531"
     CDS             complement(25746..27947)
                     /locus_tag="O2701_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HCZ4292441.1"
                     /translation="MLFVALSMIVGVLWNSSKVLSTFLFILLLYITYRKNKIVYAPIS
                     LFLIIFSAWYLHYSQQAIFNYINYIERNSQFNERAQVIQIQRQGSDTYKGRLSLKNEI
                     YPFFLTDKKNFDLKKIESRNCIVKGQFKVNDNKFVTLKLQSIVVQSCLESNRSNLIEK
                     HKQFIMNRIYDSGIKFPDRIMALITGDVKEVNEQFKERVKEIVIYHLLAVSGSHIAAI
                     VFLIYQPLKRLNLPLFVIKGITIIVLALFAQYTNYAPSAVRAIIMTTLVLVITKQIKI
                     KSIQLLAFAFIIMFILNPLVVYDIGFQFSFIISFFIMLLFPFLQQLSKLQSLFIITFI
                     AQLASFIVAIPSFHQLQWVGFLSNLIFVPYYSIILFPLSILFFITSHFIVGLTPLNYL
                     VDLSFNFHDWLLDLFTRIKQSHFSVPKFNDWIFIVFIISVYYIFWLLAKRKYILVTFW
                     TIIILTLLITFPTNSHHKITMLNVGQGDSILYEGGKNQNVLIDTGGKVIDDTKQPSYS
                     ISKYHILPTLNERGINELEYLILTHPHNDHIGEVEYIISHIKIKHIVIYNKGYSSNTL
                     MLLSKLSHKYNIKLMDVRQVSSFKLGDSSFLFFDSFIPNSRDKNEYSIITMITYQNKK
                     VLLMGDASKNNESLLLKKYNLPEIDILKVGHHGSKTSSSKEFIEMIKPKISLISSGKN
                     NMYHLPNIEVVKRLQRIRSRIYNSQQNGQVTIDLDDNLKVDSSSYGNASGL"
     gene            complement(27952..28413)
                     /locus_tag="O2701_000532"
     CDS             complement(27952..28413)
                     /locus_tag="O2701_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HCZ4292442.1"
                     /translation="MERIKWEEYFMAQSHLLALRSTCQRLSVGATIVKDNRIIAGGYN
                     GSVAGEVHCIDEGCLIEDGHCIRTIHAEMNALLQCAKQGVSTEGATIYVTHFPCLNCT
                     KSIIQAGIKRIYYAEDYHNHEYATKLLKQSGIEFKKIPFSPEYVAKYLTKG"
     gene            complement(28505..29191)
                     /locus_tag="O2701_000533"
     CDS             complement(28505..29191)
                     /locus_tag="O2701_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HCZ4292443.1"
                     /translation="MVLLYQFLLRYKDFLTQWKLYILSAVVLIMVLIGFIFWRQDDYT
                     SRNFENKDTALKQSTSENSSLSKVEDVQVKDGDNSKNKGPVYVDVKGAVKHPNVYKMT
                     SKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHEKN
                     GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKT
                     FDKLKSYFTT"
     gene            complement(29231..29947)
                     /locus_tag="O2701_000534"
     CDS             complement(29231..29947)
                     /locus_tag="O2701_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCZ4292444.1"
                     /translation="MSQYAEMSLVYDQLTQDQPYEKWFEIVKNHCKDESNILDIGCGT
                     GSLTVQLEALGNVTGMDLSVDMLTVAAQKSANVNWLEGDMTSFDLQQQFDIITIFCDS
                     LNYLQDETAVIETFINVYHHLTDSGVFIFDVHTVYKMMTLFNNQSYIDDKGDIFLAWD
                     AVQGDLPLSVYHDMTFFIRHEDETYSRFDESHFQRTFDEKTYLSWLAQVGFKHVETFT
                     DFNIDEHNEDAERLFFIAKK"
     gene            complement(29950..30303)
                     /gene="rsfS"
                     /locus_tag="O2701_000535"
     CDS             complement(29950..30303)
                     /gene="rsfS"
                     /locus_tag="O2701_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCZ4292445.1"
                     /translation="MNSQELLAIAVDAIDNKKGEDTISLEMKGISDMTDYFVVTHGNN
                     ERQVQAIARAVKEVANEQNIEVKRMEGYNEARWILIDLADVVVHVFHKDERNYYNIEK
                     LYQDAPLESYSQVAY"
     gene            complement(30304..30888)
                     /gene="yqeK"
                     /locus_tag="O2701_000536"
     CDS             complement(30304..30888)
                     /gene="yqeK"
                     /locus_tag="O2701_000536"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HCZ4292446.1"
                     /translation="MNIEKAKRLAKEKLPEKRYNHSLRVAETAIKLAEIYDGDTSKVE
                     LAGVLHDFCKYDDLGKMYQIVRQYELGNDLLSYGSEILHGPVCAAIMEHEYGINDEEV
                     LMAIKYHTTGRQQMTKTEKLIFIADYIEPGRTIPGVDDIRDMAYNQGSLDKTIYEISK
                     RTVLFLIQKDITVYNKTIDCLNYYNYSDERIKDD"
     gene            complement(30878..31447)
                     /gene="nadD"
                     /locus_tag="O2701_000537"
     CDS             complement(30878..31447)
                     /gene="nadD"
                     /locus_tag="O2701_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HCZ4292447.1"
                     /translation="MKKIVLYGGQFNPIHTAHMIVASEVFHELQPDEFYFLPSFMSPL
                     KKHHDFIDVQHRLTMIQMIIDELGFGDICDDEIKRGGQSYTYDTIKAFKEQHKDSELY
                     FVIGTDQYNQLEKWYQIEYLKEMVTFVVVNRDKNSQNVENAMIAIQIPRVDISSTMIR
                     QRVSEGKSIQVLVPKSVENYIKGEGLYEH"
     gene            complement(31450..31740)
                     /gene="yhbY"
                     /locus_tag="O2701_000538"
     CDS             complement(31450..31740)
                     /gene="yhbY"
                     /locus_tag="O2701_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCZ4292448.1"
                     /translation="MLTGKQKRYLRSLAHNIDPIFQIGKGGINENMIKQIDDTLENRE
                     LIKVHVLQNNFDDKKELAETLSEATHSELVQVIGSMIVIYRESKDNKEIELP"
     gene            complement(31744..32550)
                     /gene="aroE"
                     /locus_tag="O2701_000539"
     CDS             complement(31744..32550)
                     /gene="aroE"
                     /locus_tag="O2701_000539"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCZ4292449.1"
                     /translation="MKFAVIGNPISHSLSPVMHRANFNSLGLDDTYEALNIPIEDFHL
                     IKEIISKKELEGFNITIPHKERIIPYLDYVDEQAINAGAVNTVLIKDGKWIGYNTDGI
                     GYVKGLHSVYPDLENAYILILGAGGASKGIAYELAKFVKPKLTVANRTMARFESWNLN
                     INQISLADAEKYLAEFDIVINTTPAGMAGNNESIINLKHLSPNTLMSDIVYIPYKTPI
                     LEEAERKGNHIYNGLDMFVYQGAESFKIWTNKDADINSMKTAVLQQLKGE"
     gene            complement(32564..33664)
                     /gene="yqeH"
                     /locus_tag="O2701_000540"
     CDS             complement(32564..33664)
                     /gene="yqeH"
                     /locus_tag="O2701_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HCZ4292450.1"
                     /translation="MSDILKCIGCGAPLQSEDKNKPGFVPEHNMFRDDVICRRCFRLK
                     NYNEVQDVGLESEDFLKLLSGLADKKGIVVNVVDVFDFEGSFINAVKRIVGNKKIILA
                     ANKLDLLPKQINKRRVKEWLKRTARKYGLEADDVVLISAEKGWGIDDLLSSIANIREN
                     EDVYIVGTTNVGKSTLINKLIEASVGEKDVVTTSRFPGTTLDMIDIPLDETSFMYDTP
                     GIIQDHQMTHLVSEKELKIIMPKKEIKQRVYQLNEAQTLFFGGLARIDYVSGGKRPLV
                     CFFSNDLNIHRTKTEKANDLWRNQLGDLLTPPGNPQNFDLNEVKAVRLETGKEKRDVM
                     ISGLGFITIGPGAKVIVRVPKNVDVVLRNSIL"
     gene            complement(33665..34192)
                     /locus_tag="O2701_000541"
     CDS             complement(33665..34192)
                     /locus_tag="O2701_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HCZ4292451.1"
                     /translation="MGLVRKFFMPNSYVQSIFQIDLDKLVDKGVKGIITDLDNTLVGW
                     DVKEPTERVKAWFKEANEKGITITIVSNNNESRVASFSQHLDIDFIFKARKPMGKAFD
                     KAITKMNIRPDQTVVIGDQMLTDVFGGNRRGLYTIMVVPVKRTDGFITKFNRLIERRL
                     LRHFSKKGYITWEEN"
     gene            complement(34212..34898)
                     /gene="mtnN"
                     /locus_tag="O2701_000542"
     CDS             complement(34212..34898)
                     /gene="mtnN"
                     /locus_tag="O2701_000542"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCZ4292452.1"
                     /translation="MIGIIGAMEEEVTILKNKLTQLSEISVAHVKFYTGILKDREVVI
                     TQSGIGKVNAAISTTLLINKFKPDVIINTGSAGALDESLNVGDVLISDDVKYHDADAT
                     AFGYEYGQIPQMPVAFQSSKPLIEKVSQVVQQQQLTAKVGLIVSGDSFIGSVEQRQKI
                     KKAFPNAMAVEMEATAIAQTCYQFNVPFVVVRAVSDLANGEAEMSFEAFLEKAAVSSS
                     QTVEALVSQL"
     gene            35212..35481
                     /locus_tag="O2701_000543"
     CDS             35212..35481
                     /locus_tag="O2701_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292453.1"
                     /translation="MAENNQNSLVTKIATYGSFIAIASFVILFISIFLKFSFNIEFTA
                     TIMNICRYTLILGFILMSLPDVVDKNIKKIIFDAFIIIVFIFFLL"
BASE COUNT        11091 a         6661 c         4771 g        13156 t
ORIGIN      
        1 ccatattttt aattttattt tacaaattgg aactaatccc aatatttatt tttcaatagt
       61 gttgtttaaa tgcggtaact atcatttacc cttatacatt ttgacataat cattattcgt
      121 attattcttt aaaatcacat gctacatgcg ttctttattc ttagcaacaa tttgctgtaa
      181 atagtatttt tgtaattcta catcgccaat ccttgtagct tcccttaatt tatgattcaa
      241 tgactcaatt gtttcttgtc ctttttcatt aataacattg acataatcat caatttcatt
      301 ttcatatggt tcgtcattca aattatattg ttctaagcta attagtgttt ctctcaactc
      361 atttgaatta acatactgca cagcatcact gatattatat tgatcatttt ccgcataaaa
      421 atcatgtaag acttcgaata catatttaaa atgctgattt gtgaagttat ccttatcaac
      481 actttcataa taatttaaaa atgtatcttt atctctcatt aaatgtttta aaaatgctcg
      541 ctccgctttt tcttgacggc tcaaattgtc aaattgtgcc ataccaattg gctcaggttc
      601 aatgtaaccg ccatactcat cttctggata ataattggct ggtgcttgat tgaattgtat
      661 ttcgttagct aattgctcag gactaacatt gaaaaatggt gcaacatcat ttaaagcctt
      721 ttgttgcaaa atcgatgatt tcataagcga aatatcatga cttagttctt tcaaataacg
      781 ttcatatgaa aggtcattat gtgcaatttc atcttttaat atactcactt tataatgtgc
      841 aaatgacttt ttgtcatttt ttacaaaagc agtaaatgca tcgttgccat acttaccaat
      901 gtattcatcc ggatccatgc ctgatggcaa ttgtataaca aatacattta gcccttgctg
      961 taacaaattt tgacctgttt taagtgttgc ttcactaccc gcaaaatccc catcaaacat
     1021 taatgttata tttgatgtta actttcgtat aaaagtaata tgttcatctg acaactgtgt
     1081 acccattgtt gcaacaacgt ttttcaagcc agcagtatca gattttataa catccataaa
     1141 accttctagt aatacgattt catctaattt tctaattgat ttacgcgctt tatctaagtt
     1201 gtataacaac tttctttttt gaaagatagg tgtttcagga ctatttaagt attttggttc
     1261 ttgaccggta tatgttcgac ctgaatatcc aacaattctt ccttgcgcat ttttcaaagg
     1321 aaacataata cgatttcgaa atctatcgta ataactgaaa ttttcttcgt tacgtgataa
     1381 taatccggct tcatatgcta attcaatatc gtaacccttt ttttgaagaa aatcatgaca
     1441 aaaatgtgag ctatcgggtg caaagccaat gcctcgctct ttaataagcg catctgtaaa
     1501 accacgttct tgtaagtacg ttaatgcttg ttcgccttcg actgtctttg ttaaagcgta
     1561 ataataaaat tcttgtatta actcatgcat ttcaatcatt tgtaaatcat cagaagcaat
     1621 ttgaacattt gagttagatt gtgttgcctc aatatctaca gcaacattaa ctctatcacc
     1681 taattcttta accgcttcaa caaatgatat gtctttaatt tcttgagtaa attgaaaaac
     1741 attgccacct tttttacaac caaaacaatg acaaatttgt ttatcttcag aaactgtaaa
     1801 tgaaggtgtc ttttcatcat gaaaaggaca caaacctata taattgcgtc ctctcttttc
     1861 taattttaca tattcactta ccaagtctaa aatgtcggtt ttatctttta tttcattaat
     1921 gatcgattga tctattcgca aattaaatca cctattaagc attagttata ctattatacc
     1981 taattttcga caaaaatgaa atcatttcga tttattttgt tcaatataat ggattatatc
     2041 gtttgctgtt tcttcgattg ctttttgaga aacatcaatg acaggacatc caatttcact
     2101 tacgatttct tcaaagtaat tcaattcttc ttgaattcgt gcttctgttg catatcgagc
     2161 tgtgtcacct agtcctaatt gttttagtcg ctcttttcta atgcgattta atttttcttc
     2221 acttatttta agtgcgatac atttctttgg attaatatca tataagccat ctggcggtgt
     2281 cacttctggt acaatcggta cattcataac tttgtaactc ttatgcgcta aatactgaga
     2341 taatggtgtc tttgaagttc tcgaaatacc aagtaaaaca atatcagctt taggtaatcc
     2401 tttaggatct ttaccatcat catatttaac tgcaaactct atcgcatcaa ttttcttgaa
     2461 atatgcatca tctaatctat gaacgatacc tggctcatta taaggttttt cttcaaccga
     2521 agcagataat aaatccatta atggccccat gatatcgaca gacttcaatt ggaattctgc
     2581 tactttctca ctcatatatt gcttcatttc aggtttaata agtgtataaa caatgatagc
     2641 atttgtatct tttgcaactt gaatcacttc atcaacatct tcaaaagatt caatatatgg
     2701 atatcttaat aattcatttt tacattgctt aggattgaat tgtgaaatac ctgccctagc
     2761 aactaactct gccgtttcac ctatagaatc tgaagctacg ataattttaa ttttttccat
     2821 tacttctcac ctattcttta tataatgcta ctaataactt agctattgtt gttttggaaa
     2881 ttcttccaat tacttcatac ttttgattat ctttttttct tacaattgga atcgaatcaa
     2941 tttctttttc aatcattcta tctgctgcgt atatgactaa ttcgctttcc tctaaataag
     3001 tgacattagg catacgtgtc atatttacac tgataggtac tttatgaata tctgctccaa
     3061 tcattgaagc tcttaataaa tcttttcttg aacacacacc aacaaaatcg ttatcttcat
     3121 taataataaa taatgtactt acatcttcta aaaaaattgt acaaatagcg tcataaactg
     3181 ttgtattctc tcttagcaca acaggttgag acatatagtc cttaacctca aattgtcgaa
     3241 gtttttcatt aaaaaatttg ccttttgatt tacctgaata ataatatcca actcggggac
     3301 gcgcttctaa aaaacctgac attgttaata tcgctaaatc tggtctaagc gttgctcttg
     3361 ttaaattcaa cttatctgct atttgttcac cagtaatggg tcctttagtt ttaacaattt
     3421 cgatgattcg ttcttgtctt tgactgagtt ctataggtct tcaccccttt tttattcacc
     3481 tattattata ccgatatttc tttcatgtca caaacacatt aattttactt gcctttaaat
     3541 aatctatcaa ttagaataaa cgtacagcga gttaaggata gtgtaagctt aacaataaga
     3601 ttggcgcaac gaatcatttt aaaataaaag cgagtgacta cactaatttg ggtggaaccg
     3661 cgggttaaat acgtaactta ttttgtatta tatacaattt aaaatattaa ctcgtcccat
     3721 ggaagtaaag catatttact atgctgttat tttccatggg gcgttttcat gtatgaggag
     3781 aggtaattat ggcaaaagat atggatacaa ttgtttcatt agcaaaacac agaggttttg
     3841 tgttccctgg tagtgatatt tacggtggtt tatcaaacac atgggattat ggtcctttag
     3901 gtgttgaatt aaagaataat gttaaaaaag cttggtggca aaaattcatt acacaatcac
     3961 cgtttaacgt tggtatcgat gctgcaatct taatgaatcc aaaagtatgg gaagcttcag
     4021 gacacttaaa caacttcaac gacccaatga ttgataataa agatagtaaa attcgatatc
     4081 gcgctgataa attaattgaa gattatatgc aagatgttaa aggtgatgaa aacttcattg
     4141 ccgatggttt aagttttgaa caaatgaaaa aaattattga cgatgaaggt attgtttgtc
     4201 ctgtaagtaa aactgctaac tggactgaaa ttcgccaatt caatttaatg tttaaaacat
     4261 tccaaggtgt aactgaagat tctacaaatg aaattttctt acgtcctgaa acagcacaag
     4321 gtatttttgt aaactataaa aacgtgcaac gttcaatgcg taaaaaatta ccatttggta
     4381 tcggtcaaat tggtaaatca ttccgtaatg aaatcactcc aggtaacttc attttcagaa
     4441 caagagaatt tgaacaaatg gaacttgaat tcttctgtaa acctggagaa gaaatcgaat
     4501 ggcaaaatta ttggaaaact tttgcaagtg actggttaac aagcttaaat atgagcagtg
     4561 aaaatatgcg tttacgtgat catgatgaag atgaattatc tcattactca aatgcaacaa
     4621 ctgatattga atataaattc ccatttggtt ggggtgagtt atggggtatc gcaagtcgta
     4681 cagacttcga cttacgtaaa catgctgaac actctggtga agatttcaga taccatgatc
     4741 cagaaacgaa cgaaaaatat attccatatt gtatcgagcc atcacttggt gcagatcgtg
     4801 taacattagc tttcttatgt gatgcatatg atgaagaagg cgttgaaggt agtaaagatg
     4861 cacgtacagt tttacacttc catcctgcat tagcaccata taaagcagcg attttacctt
     4921 taagtaagaa attatctggc gaagcgatta agatttttga gcaattaagt tctaaattct
     4981 caatcgattt cgatgaatca caatctatcg gtaaaagata ccgtcgtcaa gatgaaatcg
     5041 gtacacctta ttgtgtaaca ttcgactttg attcattaga agataatcaa gttacagtac
     5101 gtgacagaga ttcaatggaa caagttcgta tgccaatctc agagttagaa gctttcttaa
     5161 ctgaaaaaac aaaattctaa ttaaaaagac tttccaccat atgtggtggc tatataattt
     5221 ataaaaagag atactggtta gtaaaattgc cagtatctct tttctattca aaaaggtatt
     5281 caaataaaca catctatgtg gctacttgaa taccttttat tattttatgt tattgttcca
     5341 atctttttaa ttggttgatt agtttctgac ttttaaaaaa catacctgca tattcacgat
     5401 ataacattaa aatgatatct gacatttcat caataatttc ttgatggata ttcaatgaat
     5461 tcattttatc tatcggtaat ttttgtaata catctaataa atatagtgtt ttattcgata
     5521 atataactgc atgtacatct ttagaagcct cttgccttga aatcgcaccg tcaaacttaa
     5581 aactataacc tattaaatct gcttgtgtgt cattaccact cacagcacag cgattaaatg
     5641 atgcagtaaa accaaatcgt ttcatgcact ttaacataac tacgactgac attaactgtg
     5701 cagatgtacc tgattctatt ttttcaagaa caaattgtaa taattgatag ttatatggtg
     5761 caatgtcacc ttcatccatt gagcgctcaa tagtttcagc tgccagagcg gcataactgc
     5821 ttacgtaaag gtccatttgt aatttataat gttgactaat aacatctaca gaatttaacg
     5881 ttcccatacc tcgccactga ttgtaaataa acaaaccata aacaaacaat tgcgtttgcg
     5941 cttgtaaacc cgtcttaact tttttagcac gccttgccat aagtggtact tttgcaccat
     6001 gctcatttaa aatcgtgata attttatcag attcaccata atcaactgct ttgatgataa
     6061 tccctttttg gcgcattaac aattatcttc accactttta agattaatct tggtcttcaa
     6121 cataaccaat ttggcgaata aagttaactt tgtttcgcca gtctctttga actttgaccc
     6181 ataattctaa ataaacttta gagcctagaa gcatttctat atcacgtctc gcacgttttc
     6241 ctacttcttt taacttttta ccgccttttc caatgacaat tcctttttgc gaatctcttt
     6301 caacatatat agttgcttcg atatgaacac gatcttcgct ttctttaacc atacggtcca
     6361 cattaacacc aatcgcatga gggatttctt cacttgtaag atgaaggatt ttttcacgaa
     6421 tgatttcacc cactacaaat tgttcaggat ggtctgaaat ttgatcatct ggataatatt
     6481 taggtccttc gggtaaatac gtctttaaaa catcaataaa atgatcgaca tttagccctt
     6541 ctaatgctga aataggtaca atctctgtaa agtccatata actttgatat tcttcaatct
     6601 ttggcattaa ttcatctgga tgcactaaat ctattttatt taatactaaa aatactggtg
     6661 tcttaacatt tttcaacatt tctataatat attcatcgcc tcgtccaatt tcctcattgg
     6721 cattaaccat aaacatgatt gcatctatct cagataatgt atttttagcg actttcatca
     6781 tatagtcacc taatttgtgt ttaggtttat gaatacctgg cgtatcaatg aatataattt
     6841 gcgcgtcatc tcttgtcata acaccttgaa ttttatttct agttgtttga gctttatcgg
     6901 acatgattgc tattttatgg ccgatcactc tattaacaaa tgttgacttt cctacatttg
     6961 gtctacctat aattgaaaca aatcctgatt tatgttctgt catttattct aaatcctttc
     7021 ctgaaaatcc aaatggtagt aactctgcga ctgtcatcat aaccatatct cctttatgat
     7081 ttgtcatata cacaggcata tcatcatcac ataattcctt caaaacttga cgacatgcac
     7141 cacaaggtga tgacggttta tctgcatcta cggttacagt tattgattca aaatcacctg
     7201 gtctgtatcc ttgagaaatt gccgatacca aactagctcg ttcagcacat atcgataatg
     7261 gataagaagc attttctaca ttggtaccat aaaaagttct accgtctttc gtttttaaat
     7321 aagcccctac tttaaattga ctgtatggcg aatatgattc ttgttgtgct tttctaactt
     7381 cttgaaaata atgaggttga taactcatat atgcctccct aaaataacgc tataaaatgt
     7441 ggtaaaaata ctattaaacc tataataaat gctaatattg aaactataag tacactaaaa
     7501 gccgcaatat ctttagcgta tttagctaaa tcatgatatt caacggtcac taaatcgaca
     7561 acatattcaa tagcagtgtt taaagcttca acagtgagaa ctaatgcaat agcaatgagt
     7621 ataaatatcc actcaatccg attaatattt agtacgagac caaagacaat agcaacaatc
     7681 attgcaaaca catgtaaaag aaatttatag tctttttgaa ttaagatttt cagcccatca
     7741 agtgcatatt taaacctttt cataattagt ctcgtgttaa tccatatgcg tttaatattg
     7801 tatcttgtcg accaaacatt tccttttcat ccgcttcagt catatgatca taacctaata
     7861 gatgcaaaaa tccatgtaat gctaaaaatc ctaattctcg ttcaaaagaa tgtccgtaat
     7921 tgtttgcttg ttcttgcgct acatccgtac agataattat atcccctaaa acacgtggta
     7981 tatcaagacc actaaaatca atctctggct catcttcttc taaagcaaat gagattacat
     8041 ctgtaacttt atctttatct ctatatgttc gattaatttc ttgtatttct tgtttatcta
     8101 caaatgtaac agaaagctca gcatcgtctt ctatatgctc ttctttttta gcaaattcta
     8161 ataaatcttc aatttgttta taccaagcat ctttaactaa gcctgtgtga tcgctaaaat
     8221 ctatcgtaaa catttaattc tctccttcat aatgttcaat gatcttactt accaatggat
     8281 gtctgactac atcgctctga tctaatttca atatacttat acctttaacg ttgtgtaacc
     8341 tactgaccgc ttccttaagt ccacttttaa cacctttagg taaatcgatt tgagtttggt
     8401 caccagtaac taccattttt gagccaaaac ctagtcttgt taaaaacatt ttcatttgcg
     8461 catgtgtcgt attctgcgcc tcatcaagaa ttacaaatgc atcttctaat gttcgtccgc
     8521 gcatatatgc aagtggcgct atttcgataa tgcctctttc aataaatcgc tctgtttgtt
     8581 cacgcccaag aacagtatat agaccatcat ataaaggtct taaatatgga tctacctttt
     8641 ctttcaaatc tcctggtaaa aatccaagtg actctcctgc ttcaacagca ggtcttgtta
     8701 atacaatacg tttaacagca cctttacgga gttgctttgc tgcataaact acagctaaga
     8761 atgtcttacc tgtaccagca ggacctatac caaatactaa atcattattt ttcatggcat
     8821 taacatatat acgttgcccc atcgttttcg cacgaatcgt cttaccaaat gcatctttag
     8881 ttatctcttc atcatataaa tctaacagat gttgaattgt gttattatgc gccattttaa
     8941 tagctgcttc aacatcttta attgtaatat tattacctaa atcaataacc ttcagcaaat
     9001 tgattaatac tgattccgct ttttctacgt tttctatttt tgtaccttta acggcaactt
     9061 cttgtcctct tgcatggatg acaacatcga aactctcttc aattgctttt aaatgttcat
     9121 cattatttcc aattaaagct tgagattggt tcatatcgtc tatttgtata attccaggca
     9181 tacacgcgct ccttttcata tatcatttat attcattcta tgatgcttta atatctactt
     9241 aattatatca tgcaattgat tgatttaaac atctttgcat tgacttactc atttttcttt
     9301 aatttaaaat aatcatttat acttttcacc ttgtcataaa agcagattca attttagact
     9361 aatcaatttt ttgctgtaat ttaaataact aatatttctc gaaaaattct ctttgttttt
     9421 taatttttct taacgtaaaa aaacaagcaa ctaccattac gatagtgctt gttttcaaaa
     9481 aattttataa ttgttttggt ttagctaaaa tttctgacca tatcatacca ttgattactt
     9541 catctttatc aaattggaac gctgatttag tcaaatcttt ttcgacattt tgagaattaa
     9601 gcagctgctt taatttcaaa cgttttgtac gttcagataa gtatttatcc tcaataatat
     9661 ctctagcacg tttttccatt ttagcaattt gcttttcttt ttctctatca atgtcagtac
     9721 gaatcgtttt aatatcatct ctaagtgatt tttctaattg acgtctaatt tcgtcagaat
     9781 tatcttgtct agaaggtcta tgtttctgct ccaccggttc tgctttaggc actgtgattg
     9841 gtttctctgt catcggtttt gtttcttgtt gttttttagg tgccataggt tctacaacag
     9901 gtttcgattt aggctcttcc tttggaagtt catcgaataa aggtggtaac gtatcatcat
     9961 attttcgttt cgatgatttc ttttcttctt catttaattc ttcacttatt tctttaaacg
    10021 ttcgctcaat ttcttcaaaa aagccacctt tttttggttc attatcggta gatgtttttt
    10081 gaggtggctt ttgattttgt ctatctttat gactattttc gcgcatagta gtaatgatag
    10141 aaatgatcac tgatatgaca aaaattagaa taccgacact catttaatca cctctcgact
    10201 taatgttcag gtgactcatc atcactttga tcagttcgtt tattaattgc atttctcatg
    10261 cctgtatcag cttcgatatt tttcaaatta taataatctt taacactgat attacctgaa
    10321 cgtaatgctt cagccatagc taatggtact tcagattcgg cttcaactac tttagcatgc
    10381 atttcttgta cacgcgcttt catttcttgc tcagttgcta cagccatagc tctacgttct
    10441 tcagcttttg cttgtgcaat atttttgtct gctaatgctt gttcagtttg taagtctgca
    10501 ccaatatttt tactaatatc aacgtcagca atatcaattg ataaaatttc aaatgcagta
    10561 cctgaatcta aacctttgct taaaactgtt ttagaaatat tatctgggtt ttcaagtact
    10621 tctgtatgat gcttactaga accaattgtt gaaacgatac cttcaccaac acgtgcgatg
    10681 attgtttctt caccagcacc accaacaagt cgagcaatat tagctctaac tgtgatacga
    10741 gctttggctt tcacttcaat accgttcatt gctacacctg cgataaatgg tgtttcaatg
    10801 actttaggat taacagacat ttgaaccgct tctaatacgt cacgtcctgc aaggtcaatt
    10861 gcagcagcac gttcgaaagg aagatcaatg tcagcacgtt gtgcagcaat attagcgtca
    10921 acaactctgt caacatttcc tcctgctaga taatgcgatt ctaattggtt tgttgttaat
    10981 gctagtcctg ctttgtgcgc tttaattaat ggcgctataa cttttcttgg agatacacga
    11041 cgtaaacgca taccaaccaa tgtacctata ccaacatgaa cgccagctgc taacgctgaa
    11101 atccataaac caatgggtac aaatgagaat aaaataagta atgcaactac tataataact
    11161 gctattacga taaaacttaa actaaacatg gtatcgctcc ttttaattaa tctacttccc
    11221 tcacaactac tcttgttcct tcaacttcaa ggatttttac cgttttattg cgcaaaataa
    11281 agttgccatc tgaaacagca tcaatacgtt cattttcaca aaaaataatc cctgcaggtc
    11341 gaagatctgt aactgtttga gcagtctttc ctacgaggtg cgagcggtta tcatgagaat
    11401 tgtaacctga ctcagaatta gttgaatctt ttaagataac tttatccaaa aacggaatct
    11461 ttctgttgaa aatcttcact aatatcaccc attctacaat cgttaaaatc aaggcaacga
    11521 taacattcgc aagcataaat agcaaattat caccgagcgt tgttatgctt atagttatca
    11581 gtatcatgcc aataatacca attactgcac caactacaaa taattcaatt acaactaata
    11641 taacgccaat tgagaatatt aagatagaat gcatattgac atttccttgg attagaaatc
    11701 ccaaaaataa aataagtaat gataatgtgg cgataatacc agctgcattg atttttttag
    11761 agtaaagttg atatacaaat cctaagaatg tcaaacaggt taatattaac gtaaaaatag
    11821 gttgaacaat tatattgcta acttgttcaa cccaagtatc tccagccgtt gattccaaga
    11881 tagttgtcat ttgtaaaaaa ttattataac tcacaatttc acctcgccct cacattaatt
    11941 atacatgaaa ttgaatatac tttatagtta taagaaccta tagaatatca atttgattat
    12001 ttataaacta tataactttt tttaattaaa aattgacaac aaaaaaacat cacatattag
    12061 tgtcagacac taacttgtga tgtttgtttt atataattaa tattcgtgtt gagggagtca
    12121 acagaggtat taattatttg aatttacgtt tacgtgcagc ttctgatttc tttttacgtt
    12181 ttacgcttgg tttttcgtaa aattcacgtt tacgtacttc ttggattgtt ccacttttag
    12241 aaactgaacg tttaaatcta cgtaacgcat cttcaagtga ttcattttta cgtactactg
    12301 ttttagacat ctgtatttcc ctccctccaa atatcaacgg aactttatag tcaattgcac
    12361 agtaaaacta tgcatttata agtataatat atctgtaatt tatggtcaat tagtaaattg
    12421 ttttttattt gaaacatatt tcacatcaaa atcacaaaga cttttagatt ttgttctaaa
    12481 aatctcttaa taatttattt aatgagaaga gttgcttata tagtaaattg tgaagccgtt
    12541 aaaacaacgt tacaaaacct atatctttaa tacggaacca tatggtatga atcaaggaat
    12601 acttaaacta aaacttctct atcagattta tttgttgcga aatcaacaac tttaattgct
    12661 tgcccttcat ttaatggata atttgcttgc gtaattttaa cttttacaat ttgacctatg
    12721 agtgattcgt caccttcaaa ttgtactttc atataattat ctgcatatcc aactaatgta
    12781 ccttctgtgt caccctgttc ctcaggaatt acttcaagca catcttgatc aaatttagac
    12841 gcatataact ttccgagttg attgcttagc gtaattaact tatgcacccg ttcattttta
    12901 atttcttcat caatttggtc atccattctt gcagctggcg tgccaattct aggagaataa
    12961 gggaaaacat gcagttcaga gaacttatgc tttacgataa aatcatatgt ttcttggaac
    13021 tcagcttcag tttcacctgg gaaaccaaca attacatcac tcgtaactgc caagtctggt
    13081 aaagctttat gcaattttgt taatcgttct gaaaatctat ccattgtata cttacgtctc
    13141 atacgtttta atactgtatc tgaaccagat tgtaatggaa tatgcaaatg acgcacaact
    13201 tttgttgaac gttctaaaac gtcaattact tcatctgtaa gttgacttgc ttcaattgaa
    13261 gaaattcgaa ttcgttctaa tccattaatc gtttcaagat cacgtaataa ttgggccaag
    13321 ttataatctt ttaaatcttg accatatcca cctgtatgaa ttcccgtcaa tacaatttcc
    13381 ttatatcctg aattcactag ttgcgtcgct tgttcaacta ctttttccgg atctcttgaa
    13441 cgcattaagc cacgagccca tggaataata cagaatgtgc agaagttgtt acaaccttct
    13501 tgaattttta atgacgcacg tgttctatct gtaaaataag ggacatctaa ttcttcatat
    13561 ttacgatttt tcatgatatt tccaacacca ttaattggtt ggcgttcttt acggaattcg
    13621 tcaatgtaac ctaatagttt atgtctatct tgtgtaccaa ctactacatc gacaccagga
    13681 atttccataa tttcagctga tgaagtttgc gcataacaac ctgttacaca gattacagca
    13741 tcaggatttt gtcttattgc acgtctaatt atttgacgac tttttttatc acccgtattc
    13801 gttactgtac aagtattaat aacaaataca tcagcattcg cttcaaagtc aacgcgctca
    13861 tagtttgctt ctttaaataa ttgccagatt gcttcagttt cataatggtt tactttacaa
    13921 cctaatgtgt gaaacgcaac tgttgacata aatattcacc ccattaattc tttttcataa
    13981 cttattgcac ttaacgcata caatggcgca gtttctgccc gtaaaattct cggcccaaga
    14041 ccaacaactg tactagtatt actaaataat gaaatttcat tttctgacaa accaccctca
    14101 ggaccaaaaa tcatcaacac tttatcctga gcattgaatt gttgtaaagt ttgcttgaaa
    14161 ttgcttaact caccatcttt tgcttcctct tcatatgcaa taagaatata gtcataatta
    14221 tcaatagtat cacaaattaa ttttaaattc gactcgaatt gaatagatgg aatcactaaa
    14281 cgatagcttt gttcagcagc ttctttaatt attttttgcc aacgctctat ctttttggca
    14341 acttttgcct cgtttaattt aacaattgaa cgttccatgc tcacagctat aaatgatgaa
    14401 gcacccaatt cagtagcttt ttgtagcaac cactcatatt tgtcagcttt gattagtcca
    14461 ctgcaaatcg taacatcaac tggcaattct gtattaatat tttgtttttc ttttaaatca
    14521 acttcaattt tatcacttgt tatgtcagca atttcacata aataaactgt ttgatcatta
    14581 aaagttaaaa taattttact accaacatca tatctcatta catttgttat atgatgaata
    14641 tcttcttttt ttgtaataaa aaaacgctga cttacatcag cgttttggtc tatgaaataa
    14701 cgttgcacat tattcactca ctttctggcc aacaagacaa acccaaccgt tgtcatgttg
    14761 ttctgaaata attttaaaac ctacacgctc catatgtgac tgtatacctt catacttctc
    14821 ttttataata ccagaagtaa taaaataacc gccttcattt agagtattat aagcatcttc
    14881 aatcatttca tcaataatat gcgctaaaat atttgctatt acaatatcaa atttttctgt
    14941 ttcgtctttc aataagttac ctggaacagc ttcaattaac gtttcacaat gatttcttct
    15001 gaagttttct ttagctacac tcactgccat ttcatcaata tccaacgctt taatacgttt
    15061 tacaccgatt agatgacttg caatacttaa tatacctgag ccagtaccaa catcaattac
    15121 tgaatgctgt ggcaatacat atgtttctat tgccttcaaa cacatacttg tagtcggatg
    15181 atcacctgtt ccaaaagcca tacctgggtc gagctcaatg caaagctctt catccgcttc
    15241 tttagcatat gtttcccaac taggaactat tgtgaacttc ttcgacgctc ggaatggatg
    15301 gaaatagttt ttccattcat tttcccaatc cgtctctgca ataatttgct cactgaattg
    15361 aacgttatgt tgatcaagtt catctaaatt taataactca tctttaattt gctgtcgcaa
    15421 cttatcatca taagtcattt cattaaaata ggctttcaat cttactccct tatctggata
    15481 atcctctttt ttcaaagcgt aaatttcacc gtatttatct tctggttggt taattaaatc
    15541 atctgaatct tctatcacga caccatttga tccatgattt tcaagtatat tggtagccaa
    15601 ttctactgct tcatgattaa taataattga aagctctgtc cagttcatac tttattctcc
    15661 cttaaagaat ctttttgctc tatctttaaa attcgaaggt tgttcattaa tttcttcacc
    15721 atttaattgg gcaaattctt tcattagttc tttttgtcta tctgttaatt tagtaggcgt
    15781 tactacttta atatcaacat ataaatctcc gtatccatag ccatgaacat tttttatacc
    15841 cttttctttt aagcggaatt gcttacctgt ttgtgtacca gcagggattg ttaacataac
    15901 ttcattattt aatgttggta tttttatttc atcgcctaaa gctgcttgtg ggaagctaac
    15961 atttaatttg taataaatat catcaccatc acgtttaaat gtttcagatg gtttaactct
    16021 aaatactacg tataaatcac cagcaggtcc tccattcacg cctggagagc cttcaccagc
    16081 taatctaatt tgttgttcat tgtcgacacc ttcaggtact ttcacttcta atttaactgt
    16141 tttattttca gtaccttttc cgtgacatgt tggacaagct tcttcaaatt cttgaccact
    16201 tccattacat ttaggacaaa cttgttcagt acgaactcta cctaaaattg tgttttgttc
    16261 tacagctaca tgaccagcgc cattacagta actacaagtc tttttacttg ttccaggctt
    16321 tgcaccatca ccatgacatg tttcgcatgt tacatcttta cggattgaaa tttcttttgt
    16381 tgtaccaaat accgcttctt caaatgttaa tgtcattgta tactgaagat catcaccttt
    16441 ttgcggtgca tttggatctc tttgtctgcc gccaccgaag aaagagctaa agatatcttc
    16501 aaaaccgccg ccaccgaagc cactaaaacc gccaaagtca gagccattga atccttgtcc
    16561 accaaaacct tgtggaccat catgtccaaa ttgatcatag cttgcgcgtt tattatcatc
    16621 acttaaaact tcataggctt cagaaatttc tttaaacttt tcatctgcac cttcttcttt
    16681 gttaatatct ggatgatatt ttttcgaaag ctttcgatac gcttttttga tttcatcttt
    16741 tgaagcatcc ttactaatgc ctaaaacttc ataataatct cttttggcca cagctatctc
    16801 tccttttctt aattaactca tatagtttaa cgtaatatgt catactatcc aaataaaaag
    16861 ccaaagccaa tgttctattg actttgactt ttcagatcat gacaacattc taattgtatt
    16921 gtttaattat tttttgtcgt cgtcttttac ttctttaaat tcagcatctt ctacagtact
    16981 atcattgttt tgaccagcat tagcaccttg tgcttgttgt tgctgttgag ccgcttgctc
    17041 atatactttt gctgataatt cttgaatcac tttttcaagt tcttcttttt tagatttaat
    17101 atcttctata tcttgacctt ctaaagcagt tttaagagcg tcttttttct cttcagcaga
    17161 ttttttatct tcttcaccga tattttcgcc taaatcagtt aaagtttttt caacttggaa
    17221 tactagactg tcagcttcgt ttcttaagtc tacttcttca cgacgttttt tatctgcttc
    17281 agcgttaact tcagcatctt ttaccatacg gtcgatttct tcgtctgata atgaagaact
    17341 tgattgaatt gtaattcttt gttctttatt tgtacctaag tcttttgcag ttacatttac
    17401 aataccgttt ttatcgatat caaacgttac ttcaatttga ggtttaccac gttcagctgg
    17461 tggaatatca gtcaattgga atctaccaag tgttttatta tccgcagcca ttggacgttc
    17521 accttgtaat acgtgtacat ctactgatgg ttgattatct actgctgttg aatagatttg
    17581 agatttagat gtaggaatcg tagtgttacg ttcaattaac gtattcatac gtccacctaa
    17641 aatttcaata cctaaagata gtggtgttac gtctaataat actacgtctt taacgtcacc
    17701 tgtgataacg ccaccttgga ttgcagctcc cattgccact acttcgtccg ggtttactcc
    17761 tttgttaggc tctttaccga tttctttttt gacagcttct tgtactgctg gaatacgagt
    17821 tgatccacca actaagataa cttcatcgat atctgagttt gttaagccag cgtctttcat
    17881 tgcttggcgt gtaggttcca ttgttcttct aattaatgaa tctgataatt cttcaaattt
    17941 agaacgagtt aagtttactt ctaagtgtaa tggaccgttt tcaccagctg agataaatgg
    18001 taatgagatt tgagtttgtg atacacctga taagtctttt ttagcttttt cagcagcatc
    18061 tttcaaacgt tgtaatgcca ttttatcttg agataagtct acgccatttt cttttttgaa
    18121 ttctgcaact aggtagtcaa taattacttg gtcaaaatca tcaccgccaa gtttgttgtc
    18181 accggctgtt gatagtactt cgaatacacc gtcacctaat tctaggatag atacgtcaaa
    18241 tgtaccgcca cctaagtcaa aaacaagaac tttttcatct ttatcagttt tgtctaaacc
    18301 atatgctaat gctgcagctg ttggttcatt aatgatacgc tcaacttcta aaccagcaat
    18361 tttaccagca tctttagttg cttgacgttc agcatcgtta aagtatgcag gtactgtaat
    18421 tacagctttg tcaactttct cacctaaata gctttcagct gtatttttta agttttgtaa
    18481 aatcatagct gagatttctt gtggtgtgta tgatttacct tcaatatcta ctttataatc
    18541 agtacccata tgacgtttaa tagattgaac agtgtttggg tttgtaatag cttgacgttt
    18601 tgctacttca ccaacttgag tttctccatt tttgaaagct acaacagatg gtgttgtacg
    18661 tgaaccttca gggttttgaa ttacttttgg ctcatcgcct tctaatactg ttacacatga
    18721 atttgttgta cctaagtcta taccaataat tttactcata ataaaattcc tccatttaat
    18781 cattaaatta atttaatttt aaacaatgtc ttttcgccaa atttaagtta ttggtttact
    18841 ttgaccattg atggtcttaa tactctatct ttaagcttgt atcctttttg tagttcttga
    18901 gtgatttcgc cagattcaaa atcagggtta tcatcttgaa ctacagcttg gtgaatattt
    18961 ggatcaaatg cttcaccttc agttttaata acttcaagac cattatcttt tagtgcgtta
    19021 atcaaacttt catgcaccat ttgtacacct ttttgaagag atttaaaagt ctcatcatca
    19081 ccttcaattt gaagtgcacg ttctatattg tctattgctg gtaaaatatc tgttaacaca
    19141 cgttgtgctt gatatgtttt gtttatttca ttttcttttt gaattctacg cttataattt
    19201 tcaaactcag cgtagagcct taaatatttc tcttcgtttt catctgctaa ttgttgaagt
    19261 tcattaattt tttgatcttt tggatctatt tcttcaataa cattctcgtc agacgtttct
    19321 tctattgctt catcttgtaa atgaccttta ctttcttcag cttgttcaac tgaatcatca
    19381 atattttgtt tgacgtttgt ttcttcaact gttgattcag tgtttttttc aactgattcg
    19441 tctttatttg tcattttctg tcctccaata ctttctaatc catcattacc aaattctatt
    19501 taataattga atgacatttt gataatgcat agctgtaggt ccaatcacag cgatttgacc
    19561 ttttaacgtt tcatcaaaat gatattgact tgttacaatt gaaatatcac ttaagctgtc
    19621 atcaatttca ttaccaattt ttacattaat atttggtgaa gatatatctt gtaataattc
    19681 tgcaattcta tttgattcta tatattgtag aatgggctga attgaagata cattactttc
    19741 attcaatgca tcaataagtt taacctttcc acccatataa atgctattac tttgattaga
    19801 aatatgatta ttcatcgtat ttaacaattt attgataaaa atttcttcct gctctgattg
    19861 aacaaaagag acaatatcat cttgtaaatt ctgattaaac tcagttagtt tgtttgtaac
    19921 aaaatttgat attgtattta gtttgtcatt attaaacggt atgtctgaag caagatgtac
    19981 atgctcaaca tgacctgatg aaaatacgat aaccattata actaaattag gattagcacg
    20041 aatcaagtgt acattattga taatatcttg tttatgatta ggatgaacaa ctaaagttgt
    20101 atattgagat atatttgata attcatctgc aaaatatgtc aatgctgatg atacatcata
    20161 ttgattctca actaacaatt gatttaatcg tcttaattta tttgtttttt gatgagatgt
    20221 ttgttcaagt aaacgattga cataatacct aaaacctaat tgtgatggcg aacgccctga
    20281 agaactatgt gtcttctcga tatagtttaa atcttcaagc tgtttcatct catttctaat
    20341 tgtagcagga ctaacattca agttatgtcg ctcaattagt gttttagaac caacgggttg
    20401 tccaaaatca acataatcct caacaattgc gtttaatata ctcaattgcc tatctgtaat
    20461 catgttttca cctcattagc actcacttat ctcaagtgct aattataatt tatcaaattg
    20521 gtcaaagtaa gtcaatgtta aagactcgaa atttcaattt ttttaatcat ttattaggaa
    20581 agcttcaaaa acctcattac ctatgacttt ccctctattt gtaagtgcaa tcacatcgtt
    20641 cttttctaca attaattcct tctcttttaa attatttatt gtttgaccaa agacactttc
    20701 aatagattgg tcaaacttct ttttgaacct actactactc acaccttcat ttaaacgcaa
    20761 cccaagaaac atttcttctt ccattctctc agtcaaagaa ggtttatttg atactaaaat
    20821 tgctttactt tctttattta tagctttgat ataatgattc actggattga tattcgtata
    20881 acgcacacca tctacataac cacttgcacc tgctccaaat ccataatatt cctcattaaa
    20941 ccagtaaacc ttattatgtt ctgattcatg gccatctaat gcaaaattag atatttcgta
    21001 ttgatggaaa ggagattgtt ctatcttaga catcagcaac tgatacatgt cagcacctaa
    21061 atcctcatta ggaagtttga gcaagccttt tctatacata ttataaaatt gggttttagg
    21121 ttcaagtatt aagccgtaac tcgaaatatg ttgaatatcc atatctaaag ctagatctaa
    21181 actttgttca aaatcttcaa tcgtctgttt cggtaaatga tacattaaat ctaaactgat
    21241 tgatttaata cctgtgtttt tagcatttaa caccgaagtg taaatatctt cagtattgtg
    21301 cgttctacct aaaacagaca ataactccgg cttgaatgtt tgaacgccca ttgaaatcct
    21361 ttttactcca tatttctcta atagttggac tttctcttta gttaactcat caggatttgc
    21421 ttcaaatgta tactcgcctg tgattgtaaa cgtatcacgt attgctttaa gtaatctttc
    21481 caactgatta atagaaaggg ccgttggtgt gccgccacct acatacatgg tctttaagat
    21541 cctatatttt gctgtagaca tttctgtgat tagtgcatct aagtactcat ctacaggttg
    21601 attctgtata aaatatttat tgaaatcaca atatgtacat attcttacac aaaatggaat
    21661 atgtatatat gcactttgta ccgtcatttt aatgccccgc ttctcagact ttttatccaa
    21721 tcggctatat cctgttggac tatctgcaat tattcttact tttaacttta acgtttaatc
    21781 aatgattgtc taatatatcg attcatttta atatttattc ttgtttagag ggagtgagat
    21841 agaaataata aagaaccact aatgatttat tatgtagtgg ctcttacaca ttagccagat
    21901 gtaatatgtc cttaaaaata aaattacatg agtaaaactc atgcataagg gagtgggaca
    21961 gaaatgatat tttcgcaaaa tttatttcgt cgtcccaccc caacttgcat tgcctgtaga
    22021 atttcttttc gaaattctct atgttggggc cccaccccaa cttgcacatt atagtaagct
    22081 gacttttcgt cagcttctgt gttggggccc cattgttata agctcatttt aactctgtcg
    22141 atggctgcca atataacatt gtaaagccca ggacgttaat ttatcctagg ctccctcttt
    22201 ttagttatta aactttattc attgtaaatt gttaattgat tttttaaaat tattcatcat
    22261 ccattttcaa tacagccaag aaagcatctt gtggaatttc aacattacca actgctttca
    22321 tcttagcttt acctgctttt tgtttttcaa gtaatttacg tttacggctt atgtcaccgc
    22381 cataacattt agctaaaacg tttttaccca ttgatttaat atttgtacgc gctacaattt
    22441 tttgtcctat tgcagcctgt acaggtactt caaattgctg tcttggaatt aacgttttaa
    22501 gtttttcaac taatgcttta ccacgttcat atgcaaaatc tctatgaact atgaagctta
    22561 gcgcatccac tttatcacca tttaataaaa tatccatctt gactaaatta ctttctttat
    22621 tttcgatgaa ttcataatca aatgatgcat atcctttagt attagattta agttgatcga
    22681 agaaatcaaa tacaacttca gctaaaggta attcataaac aatatttaca cgaatatcat
    22741 ctaaatagtc catatttata aattgtccac gtttacgttg acataattcc attactgcac
    22801 cgacatagtc atttggaacc atcatagttg cacgaacata tggctcaaat attttatcaa
    22861 ttttatcacg atctggcatt tgtgctgggt tatcaaccgt cacttctgaa ccgtccctta
    22921 aaacacattg ataaattaca gatggtgcag ttgcaattaa ttcaatacca aattctcttt
    22981 caattctttc ttgaattatt tccatgtgta acatacctaa gaaaccagtt ctataaccaa
    23041 aacctaatgc ttgtgacgat tcaggctcaa attctaatga tgcatcattc aattgtaatt
    23101 tttctaatgc ttctcttaaa tcattataat ttttgttatc tattgggaac agtccgcaat
    23161 ataccattgg attcattttc ttataacctt gcaatggttc tgatgcaggt ctactagcta
    23221 atgtgatggt gtcaccaacc ctagaatcat caacattttt aatacttgca ataatataac
    23281 caacatcacc aactgttaat tcatcaactg gaagctgctt aggtgtatta attccaactt
    23341 ctgttacttc gaactcttta ccagtggcca tcattcgaat tttatctccg gctttaacaa
    23401 caccgtccac aattcttatc gatgaaatta cccctctata tggatcatac tcagaatcaa
    23461 atattaacgc ttttagtggt gcttctgggt caccatctgg agctggcaca acttcaacta
    23521 ttttctctag tatctcttca attccaatgt tagatttagc acttgctaaa acaacatcgt
    23581 cttggtctaa acctatcata tcttcaattt cttgtttcac gcgttcaggt tctgcagcag
    23641 gtaaatcaat tttgttaata acaggcaata actctaactc attatctaat gctaaataaa
    23701 catttgctaa tgtttgtgct tcgatacctt gagccgcatc tactactaaa atcgcgccct
    23761 cacaagctgc caaagaacgt gacacttcat atgtaaaatc gacgtgtcca ggcgtatcga
    23821 ttaaatggaa tgtataagta tttccatctt tagcttcgta ctttaaacgt actgcgttta
    23881 atttgattgt aataccacgt tctctttcta aatccattga atctagtaac tgatcttgca
    23941 tatctcttgt ttcaactgat ttggtatttt ctaaaattct atcagccaat gtagattttc
    24001 cgtggtcaat atgtgctata atcgagaaat tccttatatt ctctcttctt tttaagcgtt
    24061 gctcattatc cattttatcc ttctcacttt cataaaataa acccgtatta tctttcgcat
    24121 ctttgatata ataacgtttt taataggtaa atgcaaacgc cataatcttg gatgaacttt
    24181 gaaattataa caatctttat ttaaatacat acttgcgata tgttaatatg ttaattacgt
    24241 acttataatt ataaaacgta ttgattgtgt ttccaacaca atttattaaa tctgcaaaat
    24301 tttaagctaa ccccatcaaa taaatgattg cacaacggtt agacttttgt taaaatattt
    24361 cttgttgtaa tcaaataaaa ttttgataag atgaactcac ttttaggagg tgacagaaat
    24421 ggcaaatatc aaatctgcaa ttaaacgtgt aaaaacaact gaaaaagctg aagcacgcaa
    24481 catttcacaa aagagtgcaa tgcgtacagc agttaaaaac gctaaaacag ctgtttcaaa
    24541 taacgctgat aataaaaatg aattagtaag cttagcagtt aagttagtag acaaagctgc
    24601 tcaaagtaat ttaatacatt caaacaaagc tgaccgtatt aaatcacaat taatgactgc
    24661 aaataaataa tctttttaaa taaaagttca agcgcatgct tgaactttta ttttttataa
    24721 agatagaatg aataattcca gtattaactg tttatccata tatgatgatt taagtttata
    24781 atcagtttcc gcacaagcat ctataatatt caataattca tcaagttgat aatgtcttac
    24841 ttgtcctaac gctaatttta ctctgtatgg atgtactcct attgttttag caatttgctg
    24901 tccactatat cctttttgac tcagaatctt acattgataa aataatcggt aattacttgt
    24961 aattagtgca agtaatttaa ttggttcttc tttcatagtt attaaatctt ttactaaatg
    25021 aattgcttgt tctttctttc ttttctgaat gtattcagtc agtaaaaata cattttgctc
    25081 taaacttcta ttaataattt ggttaacatc ctgcttatta attgttggtc tatcgcctaa
    25141 aaataaaatc aacttttcta tctcttgtga gacaatatta aagttaatac ctgtcaactc
    25201 aataaataaa tctaatgcat ctcttttgat atctttgaaa ttctcattta atttactttg
    25261 aatccatttt tttatttctt cttcagacat ttgctctatt tttttaagcc ttgcatgctt
    25321 ttttagagtt ttagttaact tttttctttc atcaagttta ttttgatata tctcaaagac
    25381 aatcaaattt tcgccatcat atttttcaat aaattctatt aattgatcta cattatgagc
    25441 catatctttt ggcgcttttt cacctgtaaa tatatatgca tttttaacca aaattgcttt
    25501 tttatctgaa aagaaaggca atgttaatgt ttcttcaaca attggtgcaa tctctgtttc
    25561 gtataaatta tatttcacaa agttaaagtc atctctatca cttttcaaaa attgtgatat
    25621 gatttctgca ctttgttttt caaccaattc aggcacatct ccataaatag ctacaatatt
    25681 gtcgctcatt cattacaccc tttcattcgt ttgacacatc aatagattat atcatgtttc
    25741 tgtatctata aaccacttgc atttccataa gagcttgaat caacttttaa attatcatct
    25801 aagtcaattg taacttgacc gttttgttga ctattgtaaa tgcgactgcg aatcctttgc
    25861 aatcgtttaa caacttctat attaggaaga tgatacatat tgttcttccc agaagaaatc
    25921 aaacttattt taggcttaat catctctata aattctttag aactacttgt cttgctccca
    25981 tgatgtccta cttttaaaat atcaatctcc ggcaagttat atttttttag tagtaaagat
    26041 tcattatttt tactagcatc gcccattaat aaaacttttt tattttgata tgtaatcata
    26101 gtaataatcg aatactcatt tttatctcgg ctatttggaa taaaactatc aaaaaataga
    26161 aaactactat ctccaagttt aaaactacta acttgtctta catccataag tttaatgttg
    26221 tacttatggc ttaatttcga taataacatc aatgtattac tactatatcc cttattgtat
    26281 atcactatat gtttaatttt aatatgacta ataatatatt ccacttcacc aatatggtca
    26341 ttgtgtggat gtgttaaaat tagatactct aattcattta tccctctttc atttagcgtt
    26401 ggtaaaatat gatatttaga aattgaataa ctaggttgtt tagtatcatc aatcactttc
    26461 ccacctgtat caatcaagac attttggttc ttaccgcctt catataaaat actgtctccc
    26521 tgccccacat ttaacattgt aattttgtga tgtgaatttg ttggaaacgt tattaataat
    26581 gtcagaataa ttatagtcca aaacgtaacc aatatatatt tacgtttagc caataaccaa
    26641 aatatgtaat aaacagaaat tataaatact ataaatatcc aatcattaaa cttgggaaca
    26701 gaaaaatgtg attgcttgat tcttgtgaat aggtctagta accagtcatg aaaattaaaa
    26761 cttaggtcaa ccaagtaatt tagcggcgtt aatcccacaa taaaatgact tgtaataaag
    26821 aataaaatag atagcggaaa caatataatc gaatagtacg gtacaaaaat caaattagat
    26881 aaaaatccca cccattgaag ttgatgaaag cttggaatgg caacgataaa tgaagctaat
    26941 tgtgcaataa acgtaattat gaataatgat tgtaactttg acaattgctg taaaaaagga
    27001 aaaagtagca taataaaaaa tgaaatgatg aatgaaaatt gaaatccaat atcataaaca
    27061 actagtggat ttaaaataaa cataattata aatgcaaatg ctaatagctg aatactcttt
    27121 attttaattt gcttagtaat aaccagtaca agagttgtca ttattatagc tcttacagca
    27181 ctaggtgcat aatttgtgta ttgagcaaat aaagctaata caatgattgt aattccttta
    27241 atgacaaata aaggtaaatt taatcgtttt aaaggttggt aaattaaaaa tacaattgca
    27301 gctatatgcg agccactaac tgccagcaaa tgatatataa ctatctcttt aacacgttcc
    27361 ttaaattgct cattaacttc ttttacgtca ccagtaatca atgccataat acgatccgga
    27421 aacttaatac ccgaatcata aattcgattc attataaact gtttatgttt ctcaattaaa
    27481 ttagaccggt tcgattctag gcagctttgt acaactatac tttgtaattt aagagttaca
    27541 aacttattgt cattaacttt gaattgtcct ttaacaatac aattacgact ttcaattttc
    27601 tttaaatcaa aattcttttt atctgttaaa aagaaaggat atatttcatt ttttaaactc
    27661 aacctacctt tatatgtgtc actaccttga cgttgaattt ggattacttg agcacgctca
    27721 ttaaactgag aattacgttc aatataattg atataattaa atattgcttg ttgtgaataa
    27781 tgtaaatacc atgcggagaa aatgattaaa aagagagaaa taggggcata aacgatttta
    27841 tttttacgat aagtaatata caaaagtaaa atgaaaagaa atgtagagag cactttgcta
    27901 gaattccaaa gcactcctac aatcattgat aacgcgacaa acagcaaaac attaaccttt
    27961 agtcagatat ttagcaacat attctggtga aaatggaatt tttttaaatt caataccaga
    28021 ttgtttgagt aatttagttg catattcatg gttatgataa tcttctgcat agtagatacg
    28081 ctttatacct gcttgaataa ttgactttgt acaatttagg catggaaaat gagtaacata
    28141 gattgttgca ccttcagtag atacaccttg ttttgcacat tgtaataaag cattcatttc
    28201 tgcatgtatc gttctgatac aatgtccatc ttcaattaaa catccttcat ctatacaatg
    28261 cacctcgcca gctacagagc cattataacc accagcaata atacgattat ccttaacaat
    28321 cgttgcacct acagataatc tttgacaagt tgaacgtaat gctagcaaat gactttgtgc
    28381 cataaaatat tcttcccatt tgattctttc caagatgttc accttccata cttaaaattt
    28441 agtaacattt tctaataata taagattaat cacaaaaaat aaaatttgca attaaaataa
    28501 tccattatgt cgtgaaataa gatttcagtt tatcaaaagt tttacttcca aaacctttta
    28561 cttttttcaa atcgtcaatt tcttgaaatg caccttgttg gttgcgatat tcaacaattg
    28621 cattagcttt agcttgccca actccaggaa cagacatcaa ttctgataca gatgccgtat
    28681 ttaaatttac tttagtatta tttgtgttcc catttttttc gtgcacactg tttacttcaa
    28741 tttgtggttc aacattcttt tgtcctttat gaggtatgaa aatcattttt tgatctgtta
    28801 atttttcaga caaattaatt tgacttacat ctgcatcctc caataattgt gctttatcaa
    28861 gtaaatcaac tactctatcc ttagatgtca ttttataaac attaggatgt ttaacagcac
    28921 cttttacatc gacatataca ggacccttat ttttggaatt atctccatct ttgacctgga
    28981 catcttctac tttggacaaa ctactatttt cactagtgct ttgtttcaga gcagtatctt
    29041 tattttcaaa atttcttgaa gtataatcat cttgtctcca gaatataaaa ccaattaata
    29101 ccataattaa aacaacagca cttagaatat ataacttcca ttgagttaaa aaatctttat
    29161 agcgtaataa aaattgatac aataaaacca ctcctcaact ttatacacgt ctgagcgagt
    29221 ggttttactt ttattttttc gcaatgaaaa acaatctttc tgcatcttca ttatgttcat
    29281 ctatattaaa atcagtaaat gtttcaacat gtttaaaacc aacttgagct aaccaagaca
    29341 aatatgtctt ttcatcaaat gttctctgaa aatgagactc atcaaatctt gaatatgttt
    29401 catcttcatg tcgaatgaaa aatgtcatat catgataaac acttaaaggt aaatctcctt
    29461 gcacagcatc ccatgctaaa aaaatgtccc ctttatcatc aatataactt tgattattaa
    29521 acaaagtcat cattttataa acagtatgta catcaaaaat aaatacacct gaatcagtca
    29581 gatgatgata aacattgatg aatgtttcaa tcactgcagt ttcatcttgc aaataattta
    29641 gagagtcaca aaaaatagtg atgatatcaa attgttgttg caaatcaaaa gatgtcatat
    29701 ctccttcaag ccaatttaca tttgctgatt tttgagctgc aacagtcaac atatcaacac
    29761 ttaaatccat accagtaaca ttacctaaag cttctaattg aactgttaaa ctaccagtac
    29821 cgcatccaat atctaaaata tttgattcat ctttgcagtg attttttaca atttcaaacc
    29881 atttttcata tggttgatct tgagtcaatt gatcgtacac taggctcatt tctgcatatt
    29941 gcgacataat taatacgcaa cctgactata tgattctaat ggtgcatctt gatataactt
    30001 ttcaatatta taataatttc tttcgtcttt atggaaaaca tgtaccacaa catcagctaa
    30061 gtcaattaat atccaacgcg cttcattgta tccttccata cgttttactt ctatattttg
    30121 ttcattggct acttctttca ccgctctagc aatcgcttga acttgtcgtt cattatttcc
    30181 gtgcgttaca acaaaataat ctgtcatatc gctgatacct ttcatttcta aagaaatcgt
    30241 atcttcgcct tttttattgt caattgcatc cacagcaatt gctaataatt cttgtgaatt
    30301 catttaatca tcctttattc tttcgtcact atagttataa taatttaaac agtcaatcgt
    30361 cttattatat accgtaatat ctttctgtat taaaaatagt actgtgcgtt tagaaatttc
    30421 ataaattgtc ttatctaaac taccttgatt gtatgccata tctcgaatat catcaactcc
    30481 tgggattgtt cttccaggtt cgatgtaatc tgcaataaaa atcagttttt cagtttttgt
    30541 catttgttga cgtccagtag tatggtattt gatagccatt aatacttcct catcattgat
    30601 accatattca tgttccatga ttgctgcaca cacagggcca tgcaatattt cactaccata
    30661 actcagtaga tcattaccta attcgtattg tcgaacaatt tgatacattt tacctaaatc
    30721 atcatattta cagaaatcat gtaatacacc tgctaattct actttactag tgtctccatc
    30781 ataaatttct gccaatttaa tcgctgtttc tgcaactctt aaagaatgat tataacgttt
    30841 ctctggcagt ttctcttttg caagccgttt tgctttttca atgttcatat aatccttccc
    30901 ccttaatata gttttcaacg gatttaggaa caagaacttg gatagatttc ccttcactaa
    30961 ctctttgtcg aatcattgtc gaacttatat ctaccctagg tatctgaatt gcaatcatag
    31021 cattttcaac attttgacta tttttgtctc gatttacaac tacaaaagta accatttctt
    31081 ttaagtattc aatttgatac catttctcta gttggttata ctgatccgtc ccaataacaa
    31141 agtacaactc actgtctttg tgttgctcct tgaatgcctt gatcgtgtca taggtataac
    31201 tttgaccacc acgtttaatt tcatcgtcac aaatatctcc aaaaccaagc tcgtcgataa
    31261 tcatctgtat cattgttaat ctgtgctgaa cgtctataaa atcatggtgc tttttcaatg
    31321 gagacataaa actaggtaaa aaataaaatt catctggctg taattcatga aatacttcgc
    31381 tagctactat catatgtgca gtatggatag ggttaaactg accgccgtaa agtactatct
    31441 ttttcattat tatggcaatt caatttcttt attatcttta gattctctat aaatcactat
    31501 catagatcca atcacttgca ctaattcact atgagtagct tcgcttaatg tttcagctaa
    31561 ttctttttta tcatcaaagt tattttgtag tacatgtact ttaatcaatt ctctgttttc
    31621 taacgtatca tctatttgtt taatcatatt ttcgttgata ccgccttttc caatttgaaa
    31681 aatcggatca atattgtgtg ctaaacttct taagtatctt ttttgtttgc cagtaagcat
    31741 atgttattct ccttttaatt gttgtaaaac tgctgttttc atagaattaa tatcagcatc
    31801 tttattagtc caaattttaa agctttccgc accctggtaa acaaacatat ctaagccatt
    31861 ataaatatgg tttcccttgc gctctgcttc ctctaaaata ggtgttttat acggtatata
    31921 aacaatatca ctcattaaag tattgggaga aagatgcttt aaattaataa tactttcgtt
    31981 atttccagcc atacccgctg gtgttgtatt aataacgata tcgaattcag ctaaatactt
    32041 ttcagcatct gctaatgaaa tttggtttat atttaaattc caagattcaa aacgagccat
    32101 cgttctattc gcaacagtta atttgggctt tacaaatttt gctaattcat aagcaatacc
    32161 tttacttgca ccacctgcgc ccaaaattaa aatgtatgca ttttctaaat ctggataaac
    32221 gctgtgcaat cctttaacat aaccaatacc atctgtatta taccctatcc acttgccatc
    32281 ttttatcaaa acagtgttaa ctgcacctgc attaatcgct tgttcatcaa cataatctaa
    32341 atacggtatg atacgttctt tatgaggaat tgtgatatta aagccttcta attctttttt
    32401 cgaaataatt tctttaatta aatgaaaatc ttcaattgga atatttaaag cttcataagt
    32461 atcatctaat cctaaagaat taaaatttgc tctatgcata acgggcgaca aggaatgtga
    32521 aataggattt cctataactg caaatttcat ttttttaatc accttataaa atagaatttc
    32581 ttaatacaac atcaacattt ttaggaacac gaacgattac tttagcccct ggtcctatag
    32641 ttataaagcc tagaccagag atcataacat cgcgtttctc tttgcctgtt tcaagtctaa
    32701 cagcctttac ctcattaaga tcaaaatttt gtggatttcc aggtggcgtt aataaatcgc
    32761 caagttgatt acgccataaa tcattagcct tctccgtttt agtacgatgt atattcaagt
    32821 cattagaaaa gaaacaaact aacggacgtt taccacctga tacataatct atgcgcgcta
    32881 gaccgccgaa gaataatgtc tgcgcctcat ttaattgata tacgcgttgt tttatttctt
    32941 tcttaggcat aataattttc aattcttttt cactaactaa atgcgtcatt tggtgatctt
    33001 gaataatacc tggtgtatca tacataaatg atgtttcatc taaaggaata tctatcatat
    33061 ctaaagttgt tccagggaat cttgaagttg ttactacatc tttttcacca acactagctt
    33121 caatcagttt attaatcaat gtagatttcc caacattcgt tgtccctaca atatacacat
    33181 cttcattttc tcgaatattc gcaattgatg ataataagtc gtctatgccc cagccttttt
    33241 cagctgaaat taatacgaca tcgtcagctt ccaaaccata ttttcttgct gttcgtttta
    33301 accattcttt aactcgacgt ttattaattt gtttcggcaa taaatccaat ttatttgctg
    33361 ctaaaatgat ttttttgttt ccgacaatac gtttaactgc attaataaat gatccttcaa
    33421 agtcaaatac atccacgaca ttgacgacaa tacccttttt atccgcaagt cctgataata
    33481 attttaaaaa gtcttcactt tctaatccta catcttgaac ttcgttataa tttttcaagc
    33541 ggaaacaacg tctgcaaatc acgtcatcac gaaacatatt atgctctggt acaaaaccag
    33601 gtttattttt atcttcagat tgaagtggcg caccacaacc gatacatttt aaaatgtcag
    33661 acaatcaatt ttcctcccat gtgatataac cttttttact gaaatgacgt aataatcgcc
    33721 tttcaattaa tctattaaac ttagtaataa agccatcagt tcgtttaact ggaacaacca
    33781 taattgtata tagacctcga cgattaccac caaatacatc agtaagcatt tggtcaccta
    33841 taacaacagt ttgatctggt ctgatattca tcttagttat tgctttatca aacgctttcc
    33901 ccattggctt tctcgcttta aaaataaaat cgatgtctaa atgctgacta aaactagcaa
    33961 cacgagactc attattatta gacacgattg taatagtgat tcctttttca ttagcttcct
    34021 taaaccatgc tttaacacgt tctgtaggtt ctttaacatc ccaacctact agcgtattat
    34081 ctaaatctgt aataatacct ttaacgcctt tgtccactaa cttgtctaaa tcaatttgaa
    34141 atattgattg aacatatgaa ttcggcataa aaaacttgcg aactaaaccc atttaactca
    34201 cctttacctt tttataattg agacactaat gcttcaacag tttgacttga tgatacagct
    34261 gctttttcta aaaatgcttc gaagctcatt tccgcttctc catttgctaa gtctgaaact
    34321 gcacgaacta caacaaatgg tacattaaat tgataacatg tttgtgcaat tgcagttgct
    34381 tccatttcaa ccgccatcgc atttggaaat gcttttttaa ttttttggcg ttgttcaaca
    34441 ctaccgataa agctatcacc acttacaatt aagcctactt tagctgttaa ttgttgttgt
    34501 tgtacaactt gagatacttt ttctattaaa ggtttacttg attgaaatgc taccggcatc
    34561 tgtggtattt gtccatattc ataaccaaat gctgttgcgt ctgcatcatg atattttaca
    34621 tcatcactta taagaacgtc acctacattt aaactttcat ctaaagctcc agcagaacct
    34681 gtattaataa tgacgtccgg tttaaactta ttaattaata atgtcgtaga aattgcagca
    34741 ttaacttttc caatgccact ttgggtaatc actacttctc tatcttttaa aatgccagta
    34801 taaaatttaa catgtgcaac tgaaatttcg cttaattgtg ttaatttatt ttttaatatt
    34861 gttacttctt cttccatggc accaattata ccaatcatct tttgattcac ctcttttaaa
    34921 aaatcctagc atttgttatt ttatcacatt ttaatcacta caacgacaag tatcatgatt
    34981 ttcattactg tataaaactt ttgctttatt tttataaaaa gctctttcaa taacttggta
    35041 tcataaataa ttatttttga actcataaca gtgaattcga tgcttctaat ttgtctattt
    35101 ttcaaaaact aatcatatca attacatcaa ccaatcgtgt caagaaactt tattaaataa
    35161 caggaaaatg atatgtttaa aataataatg atgaaataag aggattttac aatggctgaa
    35221 aataatcaaa atagtctcgt aacaaagata gctacatacg gcagttttat tgcaattgcg
    35281 tcgtttgtca ttttatttat aagtatattt ttaaagtttt catttaatat cgagtttaca
    35341 gcaacaatta tgaacatatg tcgctacact ctaattctag gatttatact tatgtcctta
    35401 cctgatgttg ttgataaaaa catcaagaaa attatattcg atgcatttat tatcatcgtc
    35461 tttatcttct ttttactata gtagttttat tttgtaatta cacagttaat catatttgtt
    35521 tcgtatcgaa ttcctttgtg cattaaagat aattctaata gaaaacagca gtaagacttt
    35581 tctaagtgtt agcatcctta ctgctgtttt tatttgtaac aacataatga caggtacttt
    35641 atatttcact gtcaaagatt attaatgatt aacttaatt
//
LOCUS       DALAGD010000015        35558 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.015, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000015 DALAGD010000000
VERSION     DALAGD010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 35558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35558
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.015"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(289..3135)
                     /gene="uvrA"
                     /locus_tag="O2701_000544"
     CDS             complement(289..3135)
                     /gene="uvrA"
                     /locus_tag="O2701_000544"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCZ4292454.1"
                     /translation="MKEPSIVVKGARAHNLKDIDIELPKNKLIVMTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMDKPDVDTIEGLSPAISIDQKTTSKNPRSTVAT
                     VTEIYDYIRLLYARVGKPYCPNHNIEIESQTVQQMVDRIMELEARTKIQLLAPVIAHR
                     KGSHEKLIEDIGKKGYVRLRIDGEIVDVNDVPTLDKNKNHTIEVVVDRLVVKDGIETR
                     LADSIETALELSEGQLTVDVIDGEDLKFSESHACPICGFSIGELEPRMFSFNSPFGAC
                     PTCDGLGQKLTVDVDLVVPDKDKTLNEGAIEPWIPTSSDFYPTLLKRVCEVYKINMDK
                     PFKKLTERQRDILLYGSGDKEIEFTFTQRQGGTRKRTMVFEGVVPNISRRFHESPSEY
                     TREMMSKYMTELPCETCHGKRLSREALSVYVGGLNIGEVVEYSISQALNYYKNIDLSE
                     HDQAIANQILKEIISRLTFLNNVGLEYLTLNRASGTLSGGEAQRIRLATQIGSRLTGV
                     LYVLDEPSIGLHQRDNDRLINTLKEMRDLGNTLIVVEHDDDTMRAADYLVDIGPGAGE
                     HGGQIVSSGTPQKVMKDKKSLTGQYLSGKKRIEVPEYRRPASDRKISIRGARSNNLKG
                     VDVDIPLSIMTVVTGVSGSGKSSLVNEVLYKSLAQKINKSKVKPGLYDKIEGIDQLDK
                     IIDIDQSPIGRTPRSNPATYTGVFDDIRDVFAQTNEAKIRGYQKGRFSFNVKGGRCEA
                     CKGDGIIKIEMHFLPDVYVPCEVCDGKRYNRETLEVTYKGKNIADILEMTVEEATQFF
                     ENIPKIKRKLQTLVDVGLGYVTLGQQATTLSGGEAQRVKLASELHKRSTGKSIYILDE
                     PTTGLHVDDISRLLKVLNRLVENGDTVVIIEHNLDVIKTADYIIDLGPEGGSGGGTIV
                     ATGTPEDIAQTKSSYTGKYLKEVLERDKQNTEDK"
     gene            complement(3143..5128)
                     /gene="uvrB"
                     /locus_tag="O2701_000545"
     CDS             complement(3143..5128)
                     /gene="uvrB"
                     /locus_tag="O2701_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002473941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HCZ4292455.1"
                     /translation="MVEHYPFKIHSDFEPQGDQPQAIKEIVEGIKAGKRHQTLLGATG
                     TGKTFTMSNVIKEVGKPTLIIAHNKTLAGQLYSEFKEFFPENRVEYFVSYYDYYQPEA
                     YVPSTDTFIEKDASINDEIDQLRHSATSALFERDDVIIIASVSCIYGLGNPEEYKDLV
                     VSVRVGMEMDRSELLRKLVDVQYTRNDIDFQRGTFRVRGDVVEIFPASKEELCIRVEF
                     FGDEIDRIREVNYLTGEVLKEREHFAIFPASHFVTREEKLKVAIERIEKELEERLKEL
                     RDENKLLEAQRLEQRTNYDLEMMREMGFCSGIENYSVHLTLRPLGSTPYTLLDYFGDD
                     WLVMIDESHVTLPQVRGMYNGDRARKQVLVDHGFRLPSALDNRPLKFEEFEEKTKQLV
                     YVSATPGPYEIEHTDKMVEQIIRPTGLLDPKIEVRPTENQIDDLLSEIQTRVERNERV
                     LVTTLTKKMSEDLTTYMKEAGIKVNYLHSEIKTLERIEIIRDLRMGTYDVIVGINLLR
                     EGIDIPEVSLVVILDADKEGFLRSNRSLIQTIGRAARNDKGEVIMYADKMTDSMKYAI
                     DETQRRREIQMKHNEKHGITPKTINKKIHDLISATVENDENNDKAQTVIPKKMTKKER
                     QKTIDNIEKEMKQAAKDLDFEKATELRDMLFELKAEG"
     gene            complement(5397..5633)
                     /locus_tag="O2701_000546"
     CDS             complement(5397..5633)
                     /locus_tag="O2701_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbA family protein"
                     /protein_id="HCZ4292456.1"
                     /translation="MIWYFSAAFFPCVLVVLFSVITRSKWVGTILTLILIGASIYKEY
                     FHNEWIIFIDVVSLLAGYLIIDQLEFHKHQDEDR"
     gene            complement(5630..6280)
                     /locus_tag="O2701_000547"
     CDS             complement(5630..6280)
                     /locus_tag="O2701_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCZ4292457.1"
                     /translation="MGVHQYFKRLSDMERLIRLPGKFKYFEHNVAAHSFKVTKIAQYL
                     ATVEEYHGRKINWKSLYEKALNHDFAEVFTGDIKTPVKYASSELKKLFSQVEEEMVET
                     FIEEEIPLQYRDVYKQRLQEGKDDSLEGQILSVADKIDLLYETFGEIQKRNPEELFFE
                     IYEMSLETIIQFDHLASVQDFINNIIPEMLTENFIPRTELRETTMNILNKRKEENE"
     gene            complement(6454..7293)
                     /locus_tag="O2701_000548"
     CDS             complement(6454..7293)
                     /locus_tag="O2701_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCZ4292458.1"
                     /translation="MKKSLTVTVSSVLAFLALNNAAHAQQHGTQVKTPVQHNYVSNVQ
                     AQTQSPTTYTVVAGDSLYKIALEHHLTLNQLYSYNPGVTPLIFPGDVISLVPQNKVKQ
                     TKAVKSPVRKASQAKKVVKQPVQQASKKVVVKQAPKQAVTKTVNVAYKPAQVQKSVPT
                     VPVAHNYNKSVANRGNLYAYGNCTYYAFDRRAQLGRSIGSLWGNANNWNYAAKVAGFK
                     VDKTPEVGAIFQTAAGPYGHVGVVESVNPNGTITVSEMNYAGFNVKSSRTILNPGKYN
                     YIH"
     gene            complement(7441..7548)
                     /locus_tag="O2701_000549"
     CDS             complement(7441..7548)
                     /locus_tag="O2701_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292459.1"
                     /translation="MCLLKQHMQCAFSNITYGDFQGIELIIENVLNYMV"
     gene            complement(7702..8812)
                     /gene="prfB"
                     /locus_tag="O2701_000550"
     CDS             complement(join(7702..8739,8741..8812))
                     /gene="prfB"
                     /locus_tag="O2701_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667688.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCZ4292460.1"
                     /translation="MELSEIKRNIDKYNQDLTQIRGSLDLENKETNIQEYEEMMAEPN
                     FWDNQTKAQDIIDKNNALKAIVNGYKTLQAEVDDMDATWDLLQEEFDEEMKEDLEQEV
                     INFKAKVDEYELQLLLDGPHDANNAILELHPGAGGTESQDWANMLFRMYQRYCEKKGF
                     KVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFAS
                     CDVIPDFNNDEIEIEINPDDITVDTFRASGAGGQHINKTESAIRITHHPSGIVVNNQN
                     ERSQIKNREAAMKMLKSKLYQLKLEEQAREMAEIRGEQKEIGWGSQIRSYVFHPYSMV
                     KDHRTNEETGKVDAVMDGDIGPFIESYLRQTMSHD"
     gene            complement(8981..9085)
                     /locus_tag="O2701_000551"
     CDS             complement(8981..9085)
                     /locus_tag="O2701_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292461.1"
                     /translation="MLWEESFYFTSVKYFKKCEEVVKRCYVLSFKKSV"
     gene            complement(9127..11658)
                     /gene="secA"
                     /locus_tag="O2701_000552"
     CDS             complement(9127..11658)
                     /gene="secA"
                     /locus_tag="O2701_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCZ4292462.1"
                     /translation="MGFLSKILDGNNKEIKQLGKLADKVIALEEKTAILTDEEIRNKT
                     KQFQTELADIDNVKKQNDYLDKILPEAYALVREGSKRVFNMTPYKVQIMGGIAIHKGD
                     IAEMRTGEGKTLTATMPTYLNALAGRGVHVITVNEYLSSVQSEEMAELYNFLGLTVGL
                     NLNSKTTEEKREAYAQDITYSTNNELGFDYLRDNMVNYSEDRVMRPLHFAIIDEVDSI
                     LIDEARTPLIISGEAEKSTSLYTQANVFAKMLKQDEDYKYDEKTKAVHLTEQGADKAE
                     RMFKVENLYDVQNVDVISHINTALRAHVTLQRDVDYMVVDGEVLIVDQFTGRTMPGRR
                     FSEGLHQAIEAKEGVQIQNESKTMASITFQNYFRMYNKLAGMTGTAKTEEEEFRNIYN
                     MTVTQIPTNKPVQRNDKSDLIYISQKGKFDAVVEDVVEKHKAGQPVLLGTVAVETSEY
                     ISNLLKKRGIRHDVLNAKNHEREAEIVAGAGQKGAVTIATNMAGRGTDIKLGEGVEEL
                     GGLAVIGTERHESRRIDDQLRGRSGRQGDKGDSRFYLSLQDELMIRFGSERLQKMMSR
                     LGLDDSTPIESKMVSRAVESAQKRVEGNNFDARKRILEYDEVLRKQREIIYNERNSII
                     DEEDSSQVVDAMLRSTLQRSINYYINTADDEPEYQPFIDYINDIFLQEGDITEDDIKG
                     KDAEDIFEVVWAKIEAAYQSQKDILEEQMNEFERMILLRSIDSHWTDHIDTMDQLRQG
                     IHLRSYAQQNPLRDYQNEGHELFDIMMQNIEEDTCKFILKSVVQVEDNIEREKTTEFG
                     EAKHVSAEDGKEKVKPKPIVKGDQVGRNDDCPCGSGKKFKNCHGK"
     gene            complement(12072..12644)
                     /gene="raiA"
                     /locus_tag="O2701_000553"
     CDS             complement(12072..12644)
                     /gene="raiA"
                     /locus_tag="O2701_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCZ4292463.1"
                     /translation="MIRFEIHGDNLTITDAIRNYIEEKIGKLERYFNDVPNAVAHVKV
                     KTYSNSATKIEVTIPLKNVTLRAEERNDDLYAGIDLINNKLERQVRKYKTRINRKSRD
                     RGDQEVFVAELQEMQETQVDNDAYDDNEIEIIRSKEFSLKPMDSEEAVLQMNLLGHDF
                     FVFTDRETDGTSIVYRRKDGKYGLIQTSEQ"
     gene            complement(12705..13379)
                     /locus_tag="O2701_000554"
     CDS             complement(12705..13379)
                     /locus_tag="O2701_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HCZ4292464.1"
                     /translation="MNNCLSCGAKLYENITIYNLFKKPNRLCDRCKENWDNIKLDIKA
                     RRCSRCLKHLNQDEAYCLDCKFLSAHFNLMEQLYCQFQYDGLMKEMIHQYKFLKDYYL
                     CELLAHLIEIPQTSYDYIVPIPSSPAHDLSRTFNPVEAVLKAKGIRFDKILKMSNRPK
                     QSHLTKKERLADENPFIIDTELDLNGKEILLVDDIYTTGLTIHRAGCKLYAKNIRKFK
                     VFAFAR"
     gene            complement(13372..14454)
                     /locus_tag="O2701_000555"
     CDS             complement(13372..14454)
                     /locus_tag="O2701_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCZ4292465.1"
                     /translation="MDNVTRYKITESSQSSSQAYYHLSFELSEQQSYASEHIVRAIRK
                     RQTILLYAVTGAGKTEMMFQGIQYARIQGDNIAIVSPRVDVVVEISKRIKDAFLNEDI
                     DILHQQSRQQFEGHFVVCTVHQLYRFKQHFDTIFIDEVDAFPLSMDKNLQQALKSSSK
                     VEHATIYMTATPPKQLLSEIPHENIIKLPARFHKKSLPVPKYRYFKLNNKKIQKMLYR
                     ILQDQINNQRYTLVFFNNIETMIKTFSVYKQKITKLTYVHSEDVFRFEKVEQLRNGHF
                     DVIFTTTILERGFTMANLDVVVIDAHQYTQEALIQIAGRVGRKLECPTGKVLFFHEGV
                     SMNMIQAKKEIQRMNKLALKRGWIDE"
     gene            complement(14818..15684)
                     /gene="fakB1"
                     /locus_tag="O2701_000556"
     CDS             complement(14818..15684)
                     /gene="fakB1"
                     /locus_tag="O2701_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB1"
                     /protein_id="HCZ4292466.1"
                     /translation="MKIAVMTDSTSYLSQDLIDKYNIQIAPLSVTFDDGKNFTESNEI
                     AIEEFYNKMASSQTIPTTSQPAIGEWITKYEMLRDQGYTDIIVICLSSGISGSYQSSY
                     QAGEMVEGVNVHAFDSKLAAMIEGCYVLRAIEMVEEGYEPQQIIDDLTNMREHTGAYL
                     IVDDLKNLQKSGRITGAQAWVGTLLKMKPVLKFEDGKIIPEEKVRTKKRAIQTLEKKV
                     LDIVKDFEEVTLFVINGDHFEDGQALYKKLQDDCPSAYQVAYSEFGPVVAAHLGSGGL
                     GLGYVGRKIRLT"
     gene            15828..16469
                     /locus_tag="O2701_000557"
     CDS             15828..16469
                     /locus_tag="O2701_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HCZ4292467.1"
                     /translation="MTQNIITVKKEHIIENVISKSRFIAHIKPVQNEDEAKAFIAAIK
                     KEHKDATHNCSAYTIGPEMNIQKANDDGEPTGTAGVPMLDILKKLDVHNACVVVTRYF
                     GGIKLGGGGLIRAYSGAVRDVIYDIGRVELREAVPCIVTLNYDQTGKFEYELASTHFM
                     LRNQTYTDKVSYYIDVVKSDYEAFINFLNQMTAGNFDLTEETPKQLPFDIPTE"
     gene            complement(16639..17694)
                     /locus_tag="O2701_000558"
     CDS             complement(16639..17694)
                     /locus_tag="O2701_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HCZ4292468.1"
                     /translation="MVTLLLVAVTMIVSLTITPIVIAISKRLNLVDKPNFRKVHTKPI
                     SVMGGTVILFSFLIGIWIGHPIETEIKPLIIGAIIMYVLGLVDDIYDLKPYIKLAGQI
                     AAALVVAFYGVTIDFISLPMGTTIHFGFLSIPITVIWIVAITNAINLIDGLDGLASGV
                     SAIGLITIGFIAILQANIFITMICCVLLGSLIGFLFYNFHPAKIFLGDSGALMIGFII
                     GFLSLLGFKNITIIALFFPIVILAVPFIDTLFAMIRRVKKGQHIMQADKSHLHHKLLA
                     LGYTHRQTVLLIYSISILFSLSSIILYVSPPLGVVLMFVLIIFSIELIVEFTGLIDNN
                     YRPILNLISRKSSHKEE"
     gene            18012..19082
                     /locus_tag="O2701_000559"
     CDS             18012..19082
                     /locus_tag="O2701_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCZ4292469.1"
                     /translation="MFEAFIYNISVIVAGIYLFHRLQYSENKRMVFSKAYVTVLMTIV
                     SLLLSVYPIPYREDYLIHLTFVPLLFLGRFTNMVYTLSATVIVAIVEIVVFNNSIMYG
                     VTLIVIAAVTSAIGPFLKQNDVLSLLILNVVTIIILFGVALVSPIYTLSEVIILIPIS
                     LIITLASAITFVDIWHFFSLVNRYENEDKYDYLTGLGNVKEFDRHLNEISRKAEKEHQ
                     SIALLLIDIDGFKDVNDTYSHKSGDAVLKQMSQLLKNYVPNQFKIFRNGGEEFSVVIH
                     NYSLDQSVKLAENIRSGVEKSSFHLPNKEVIKLSVSIGVGYLTDDDPKSQRKVFKDAD
                     DMVHVAKNQGRNKVMFNPIINL"
     gene            19278..20039
                     /locus_tag="O2701_000560"
     CDS             19278..20039
                     /locus_tag="O2701_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCZ4292470.1"
                     /translation="MPDSITIIDENKVIDVVLIAGRILLESGAETYRVEDTMNRIAHS
                     YGLHNTYSFVSSTAIIFSLNDRTSTRLIRVQERTTDLEKIALTNSLSRKISNKELTID
                     EAKSEFIHLQHASLQYSFLTNFFAAAIACGFFLFMFGGVASDCWIAVIAGGSAFLTFS
                     FVQRYIQIKFFSEFVAAAVVISIAATFTKLGIATNQDIITIASVMPLVPGILITNAIR
                     DLLAGELLAGMSRGVEAALTAFAIGAGVAIVLLII"
     gene            20057..20551
                     /locus_tag="O2701_000561"
     CDS             20057..20551
                     /locus_tag="O2701_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCZ4292471.1"
                     /translation="MLFYLFHFTISFISTVLFSIIFNAPKRLLVACGFVGAIAWTIYQ
                     LTVDLEFGKVGASFLGSLILGLMSHTMSRRYKRPVIIFIVPGIIPLVPGGAAYQATRF
                     LVSNDYTSAVNTFLEVTLISGAIAFGILVSEILYYLYTRIKQLYGKIKGKTYKKSYNM
                     NNRV"
     gene            20565..21791
                     /gene="pepT"
                     /locus_tag="O2701_000562"
     CDS             20565..21791
                     /gene="pepT"
                     /locus_tag="O2701_000562"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCZ4292472.1"
                     /translation="MKNQLIDRLTRYTTIDTQSDPKSTTTPSTEKQWDLLHLLEKELQ
                     QLGLPTDLDENGYLFATLESNIDVDVPTVGFLAHVDTSPDFNASNVKPQIIENYDGKP
                     YKLGNTKRVLDPKVFPELNSLVGHTLMVTDGTSLLGADDKAGIVEIMEAICYLQEHPE
                     IKHGTIRIGFTPDEEIGRGPHKFDVDRFNADFAYTMDGSQYGELQYESFNAAEAVITC
                     HGVNVHPGSAKNAMVNAIRLGEQFDSLLPDSEVPERTEGYEGFYHLMNFEGTVEKATL
                     QYIIRDHDKKQFELRKKRILEIRDDINAHFENYPVKVDISDQYFNMAEKILPLPHIID
                     IPKRVFAKLDIPANTEPIRGGTDGSQLSFMGLPTPNIFTGCGNFHGPYEYASIDVMEK
                     AVQVIIGIVEDIAENH"
     gene            complement(21980..23104)
                     /locus_tag="O2701_000563"
     CDS             complement(21980..23104)
                     /locus_tag="O2701_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCZ4292473.1"
                     /translation="MKVLVAMDEFHGIISSYQANRYVEEAVASQIETADVVQVPLFNG
                     RHELLDSVFLWQSGQKYRIPVHDADMNEVEGVYGQTDTGMTVIEGNLFLKGKKPIVER
                     TSYGLGEMIKHALDNDAKHVVISLGGIDSFDAGAGMLQALGAQFYDDEGRVVDMRQGA
                     GVIKYIRRMDMSNLHPKMETARIQVMSDFSSRLYGKQSEIMQTYDAYQLNHNQAAEID
                     NLIWYFSELFKSELKIAIGPVERGGAGGGIAAVLNGLYQAEILTSHALVDQLTHLENL
                     VEQADLIIFGEGLNENDQLLETTTLRIAELCHKHQKVAIAICATAEKFDLFESQGVTA
                     MFNTFIDMPETYTDFKMGLQIRHYTVQSLKLLKTHFNVEV"
     gene            complement(23529..23849)
                     /gene="ytxJ"
                     /locus_tag="O2701_000564"
     CDS             complement(23529..23849)
                     /gene="ytxJ"
                     /locus_tag="O2701_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HCZ4292474.1"
                     /translation="MAIKLSSIDQFEQVIEENKYVFVLKHSETCPISANAYDQFNKFL
                     YERDMDGYYLIVQQERDLSDYIAKKTNVKHESPQAFYFVNGEMVWNRDHGDINVSSLA
                     QAEE"
     gene            complement(24003..24881)
                     /locus_tag="O2701_000565"
     CDS             complement(24003..24881)
                     /locus_tag="O2701_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EMYY motif lipoprotein"
                     /protein_id="HCZ4292475.1"
                     /translation="MKKIVIIAVLAILFVVISACGNKEKEAQHQFTKQFKDVEQKQKE
                     LQHVMDNIHLKEIDHLSKTDTTDKNSKEFKALQEDVKNHLIPKFEAYYKSAKNLPDDT
                     MKVKKLKKEYMTLANEKKDAIYQLKKFIGLCNQSIKYNEDILDYTKQFEKNRYKVESE
                     IKLADNKSEATNLTTKLEHNNKALRDTAKKNLDDSKENEVKGAIKNHIMPMIEKQITD
                     INQTNISDKHVNNARKNAIEMYYSLQNYYNTRIETIKVSEKLSKVDVDKLPKKGIDIT
                     HGDKAFEKKLEKLEEK"
     gene            25001..25519
                     /locus_tag="O2701_000566"
     CDS             25001..25519
                     /locus_tag="O2701_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HCZ4292476.1"
                     /translation="MYSNVQPFINSDLTNCFQSQYTEIESLLFNLLDSPVKYTKHIGG
                     THHFKYSTEPILDILVGVENLHDITSLDEKRLNYVGFYRLHHKYTKKVMMAKFNNFHE
                     LKQQVRLHIIQMNTQTFESYLALDYLLSTNDDIALQFNTKKHQILQHSLTIRQYEMQK
                     QQYFEKLCKKYL"
     gene            25646..26569
                     /locus_tag="O2701_000567"
     CDS             25646..26569
                     /locus_tag="O2701_000567"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCZ4292477.1"
                     /translation="MINKDIYQALQQLIPNEKIKVDEPLKRYTYTKTGGNADFYITPT
                     KNEEVQAVVKYAYQNEIPVTYLGNGSNIIIREGGIRGIVISLLSLDHIEVSDDAIIAG
                     SGAAIIDVSRVARDYALTGLEFACGIPGSIGGAVYMNAGAYGGEVKDCIDYALCVNEQ
                     GSLIKLTTKELELDYRNSIIQKEHLVVLEAAFTLAPGKMTEIQAKMDDLTERRESKQP
                     LEYPSCGSVFQRPPGHFAGKLIQDSNLQGHRIGGVEVSTKHAGFMVNVDNGTATDYEN
                     LIHYVQKTVKEKFGIELNREVRIIGEHPKES"
     gene            26587..26901
                     /locus_tag="O2701_000568"
     CDS             26587..26901
                     /locus_tag="O2701_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292478.1"
                     /translation="MPKVYGSLIDTETRCRHYFTEEDIIAIKFKCCNKYYPCYKCHNE
                     FEKHAIKRWSEPSFNEKAILCGVCKHELTINEYMMVERCPNCQSRFNNRCKYHYHIYF
                     EI"
     gene            complement(27218..28246)
                     /locus_tag="O2701_000569"
     CDS             complement(27218..28246)
                     /locus_tag="O2701_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HCZ4292479.1"
                     /translation="MKKTVLYLVLAVMFLLAACGNNSDKEQSKSETKGSKDTVKIENN
                     YKMRGEKKDGSDAKKVKETVEVPKNPKNAVVLDYGALDVMKEMGLSDKVKALPKGEGG
                     KSLPNFLESFKDDKYTNVGNLKEVNFDKIAATKPEVIFISGRTANQKNLDEFKKAAPK
                     AKIVYVGADEKNLIGSMKQNTENIGKIYDKEDKAKELNKDLDNKIASMKDKTKNFNKT
                     VMYLLVNEGELSTFGPKGRFGGLVYDTLGFNAVDKKVSNSNHGQNVSNEYVNKENPDV
                     ILAMDRGQAISGKSTAKQALNNPVLKNVKAIKEDKVYNLDPKLWYFAAGSTTTTIKQI
                     EELDKVVK"
     gene            complement(28365..29126)
                     /locus_tag="O2701_000570"
     CDS             complement(28365..29126)
                     /locus_tag="O2701_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4292480.1"
                     /translation="MIQVENLTKTINNQMILEDISIDIEKGKLTSLIGPNGAGKSTLL
                     SAICRLIRFENGEVKIDGQLMSDYKNNDLSKKISILKQTNHTEMNITVEQLVNFGRFP
                     YSKGRLTKEDHDIVNDALDLLQLQDIRNRNIKSLSGGQRQRAYIAMTIAQDTEYILLD
                     EPLNNLDMKHAVQIMQTLKMLAHKMNKAIVIVLHDINFASCYSDQIVALKNGQLVKSD
                     LKDNVIQSSVLSDLYDMNIQIEHIRNQRICLYFKD"
     gene            complement(29123..30079)
                     /locus_tag="O2701_000571"
     CDS             complement(29123..30079)
                     /locus_tag="O2701_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCZ4292481.1"
                     /translation="MRNNNKKIMLLIAVTLLISMLYLFVGIDFEIFEYQFSSRLRKFI
                     LIILVGAAIATSVVIFQAITNNRLLTPSIMGLDAVYLFIKVLPVFLFGIQSVWVTNVY
                     LNFILTLITMVLFALILFQGIFKIGHFSIYFILLIGVLLGTFFRSITGFIQLIMDPES
                     FLAIQSSMFANFNASNSNLVTFSAVLLVILLVITILLLPYLDVLLLGRAEAINLGISY
                     EKLTRILLVIVSVLVSVSTALVGPITFLGLLTVNLAHELMKTYEHKYILIATICLSWI
                     SLFSAQWVVENVFEATTEMSILIDLIGGSYFIYLLVRRRNAQ"
     gene            complement(30066..31037)
                     /locus_tag="O2701_000572"
     CDS             complement(30066..31037)
                     /locus_tag="O2701_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4292482.1"
                     /translation="MKYLLKGNILLLLLILLTIISLFIGVSELSIKDLLHLTESQRNI
                     LFSSRIPRTMSILIAGSSLALAGLIMQQMMQNKFVSPTTAGTMEWAKLGILIALLFFP
                     TGHILLKLVFAVICSICGTFLFVKIIDFIKVKDVIFVPLLGIMMGGIVASFTTFISLR
                     TNAVQSIGNWLNGNFAIITSGRYEILYLSIPLLALTYLFANHFTIVGMGKDFTNNLGL
                     SYEKLINIALFITATITALVVVTVGTLPFLGLVIPNIISIYRGDHLKNAIPHTMMLGA
                     IFVLFSDIVGRIVVYPYEINIGLTIGVFGTIIFLILLMKGRKNYAQQ"
     gene            complement(31076..31231)
                     /locus_tag="O2701_000573"
     CDS             complement(31076..31231)
                     /locus_tag="O2701_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292483.1"
                     /translation="MFYFTLSKQNTHSEKLNFSLIANINKIDTKLFESLLKLWSKNAT
                     INEKYYQ"
     gene            complement(31745..32716)
                     /gene="nrdF"
                     /locus_tag="O2701_000574"
     CDS             complement(31745..32716)
                     /gene="nrdF"
                     /locus_tag="O2701_000574"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCZ4292484.1"
                     /translation="MIAVNWNTQEDMTNMFWRQNISQMWVETEFKVSKDIASWKTLSE
                     AEQDTFKKALAGLTGLDTHQADDGMPLVMLHTTDLRKKAVYSFMAMMEQIHAKSYSHI
                     FTTLLPSSETNYLLDEWVLEEPHLKYKSDKIVANYHKLWGKEASIYDQYMARVTSVFL
                     ETFLFFSGFYYPLYLAGQGKMTTSGEIIRKILLDESIHGVFTGLDAQHLRNELSESEK
                     QKADQEMYKLLNDLYLNEESYTKMLYDDLGITEDVLNYVKYNGNKALSNLGFEPYFEE
                     REFNPIIENALDTTTKNHDFFSVKGDGYVLALNVEALQDDDFVFDNK"
     gene            complement(32834..34939)
                     /gene="nrdE"
                     /locus_tag="O2701_000575"
     CDS             complement(32834..34939)
                     /gene="nrdE"
                     /locus_tag="O2701_000575"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCZ4292485.1"
                     /translation="MKTMEEKKYNHIELNNEVTKRREDGFFSLEKDQEALVAYLEEVK
                     DKTIFFDTEIERLRYLVDNDFYFNVFDIYSEADLIEITDYAKSIPFNFASYMSASKFF
                     KDYALKTNDKSQYLEDYNQHVAIVALYLANGNKAQAKQFISAMVEQRYQPATPTFLNA
                     GRARRGELVSCFLLEVDDSLNSINFIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIK
                     GVAKGVLPIAKSLEGGFSYADQLGQRPGAGAVYLNIFHYDVEEFLDTKKVNADEDLRL
                     STISTGLIVPSKFFDLAKEGKDFYMFAPHTVKEEYGVTLDDIDLEKYYDDMVANPNVE
                     KKKKNAREMLNLIAQTQLQSGYPYLMFKDNANRVHPNSNIGQIKMSNLCTEIFQLQET
                     SIINDYGIEDEIKRDISCNLGSLNIVNVMESGKFRDSVHSGMDALTVVSDVANIQNAP
                     GVRKANSELHSVGLGVMNLHGYLAKNKIGYESEEAKDFANIFFMMMNFYSIERSMEIA
                     KERGIKYQDFEKSDYANGKYFEFYTTQEFEPQFEKVRELFDGMAIPTSEDWKKLQQDV
                     EQYGLYHAYRLAIAPTQSISYVQNATSSVMPIVDQIERRTYGNAETFYPMPFLSPQTM
                     WYYKSAFNTDQMKLIDLIATIQTHIDQGISTILYVNSEISTRELARLYVYAHYKGLKS
                     LYYTRNKLLSVEECTSCSI"
     gene            complement(34902..35300)
                     /gene="nrdI"
                     /locus_tag="O2701_000576"
     CDS             complement(34902..35300)
                     /gene="nrdI"
                     /locus_tag="O2701_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCZ4292486.1"
                     /translation="MKIIYFSFTGNVRRFIKRTELENTLEITAENCMEPVHEPFIIVT
                     GTIGFGEVPEPVQSFLEVNHQYIRGVAASGNRNWGLNFAKAGRTISEEYNVPLLMKFE
                     LHGKNKDVIEFKNKVGNFNENHGREKVQSY"
BASE COUNT        11282 a         6462 c         5041 g        12773 t
ORIGIN      
        1 tactttttct ttagaaatta gtatttctta tgcatgagtt gcactcatgc attcctattt
       61 ttaagtacac aatagctgtg gctaatgaca aagaaccact acataataaa tcattagtga
      121 ttctttatca tttctgtccc actcccagtt cgatttatgt ttaactactc tagattgacc
      181 ctatcttaac ataattcttt ttacgcatct tttctttcta ctattcatta ctaaattaat
      241 aaaaagaagc gaaggataaa tttataacat ccttcacttc ttttaatctt atttatcttc
      301 agtattttgt ttatctcgtt caagtacttc ttttaaatac tttcctgtat atgatgactt
      361 tgtctgagca atatcttcgg gtgtgccagt cgcaacaata gtaccaccgc cactaccacc
      421 ttcaggacct aagtctataa tatagtctgc tgttttgata acatctaggt tatgttcaat
      481 aattacaaca gtatcaccat tttcaactaa tcggtttaat acttttaata atctactaat
      541 atcgtcaaca tgtaaccctg ttgtcggttc atctaggata taaatagatt taccagttga
      601 acgtttatga agttcagatg caagtttcac acgttgagcc tcaccacctg ataacgttgt
      661 agcttgttga cctaatgtga cgtatccaag accaacatca actagtgttt gtaacttgcg
      721 cttaatctta ggaatatttt caaaaaattg tgttgcttct tcaacagtca tttctaaaat
      781 gtcagcaata tttttacctt tgtaagtaac ctctagtgtc tcacgattat atcgtttacc
      841 atcacacact tcacaaggaa cataaacatc aggtaaaaaa tgcatttcaa ttttaataat
      901 accgtcacct ttacaagctt cacagcgtcc accttttaca ttaaaactaa aacgcccttt
      961 ttgatatcct cgaattttag cttcatttgt ttgcgcaaac acatcacgta tatcatcaaa
     1021 cacaccagta tatgttgctg gattagagcg tggcgttcta cctattggtg attgatcaat
     1081 atcaataatt ttatcaagtt gatcaatacc ttcaatctta tcgtacaatc ctggctttac
     1141 tttagattta ttaatttttt gagctaatga tttgtataat acttcattta ctaatgagct
     1201 tttaccagaa cctgatacac ctgtaacaac cgtcatgatt gatagtggta tgtccacatc
     1261 aaccccttta agattgttgc ttctagctcc acgtatagaa attttacgat ctgaagccgg
     1321 tctgcgatat tcaggtactt caatacgttt cttaccactc aagtattgtc ctgttaatga
     1381 ttttttatct ttcattacct tttgaggagt accactagac acaatctgcc ctccatgttc
     1441 accagcacca ggacctatat ccactaagta atcagccgca cgcattgtat catcatcgtg
     1501 ttcaactaca attaaagtat ttcctaaatc tctcatttct ttaagtgtat taattaatcg
     1561 atcattatct ctttgatgca gtccaattga tggctcatct aatacatata agacaccagt
     1621 caaacgcgac ccaatttgcg ttgctaatcg aatacgttgt gcttcaccac ctgaaagtgt
     1681 acctgaagct ctgtttaacg ttaaatattc aagtcccaca ttatttaaaa aagtgagtcg
     1741 ggaaataatt tctttcaata tttgatttgc aatcgcttga tcatgttctg acaaatcaat
     1801 gtttttataa tagttcagcg cttgactgat tgaatattcg actacttcac caatatttaa
     1861 accacctaca taaacagata acgcttcacg actcaatcgc tttccatgac aagtttcgca
     1921 aggtagttca gtcatatatt tactcatcat ttcacgtgta tattctgaag gagattcatg
     1981 gaatcgtcta cttatattag gaactacacc ctcgaaaacc attgttcgtt ttctagtacc
     2041 accttgacgt tgtgtaaatg taaattcaat ttctttgtca ccagaaccat acaataaaat
     2101 atcacgttga cgttctgtta actttttaaa aggtttatcc atattgattt tataaacttc
     2161 acaaacacgt tttaacaatg ttggataaaa atcagaactc gtcggtatcc aaggttctat
     2221 tgcaccttcg tttagcgtct tatctttgtc gggaacaacc aagtctacat cgactgttaa
     2281 cttttggcct aagccatcac atgtcggaca agcaccaaaa ggactgttaa agctaaacat
     2341 tcttggttct aactctccga ttgaaaatcc acatatagga caagcatggc tttctgaaaa
     2401 cttaaggtct tccccgtcaa tgacatcgac tgttaattgt ccttctgaaa gctctaaggc
     2461 agtttctata gagtcagcta gtcgtgtttc aattccatct ttaacaacta atcggtctac
     2521 aacaacttct attgtatgat tcttgttctt atctaaagta ggtacatcat ttacatcaac
     2581 aatttcgcca tcgattctta aacgtacata acctttttta ccaatatctt cgattagctt
     2641 ttcatgacta cctttacgat gagcgatgac aggtgctaat aattgaatct ttgtacgtgc
     2701 ctctaattcc ataatgcggt caaccatttg ttgtactgtt tgcgattcaa tttctatatt
     2761 gtgatttgga cagtaaggtt taccaacacg tgcatataac aaacgtatat aatcatatat
     2821 ttctgttact gttgctacag ttgatcttgg atttttactt gttgtttttt gatctattga
     2881 aattgctggc gataatcctt caattgtatc aacatctggt ttgtccattt ggcctaaaaa
     2941 ttgacgcgca taggcactta atgattcaac ataacgtcgt tgtccttcag catatatagt
     3001 atcgaatgct aatgacgatt tacctgaccc agataaacct gtcataacaa ttaatttatt
     3061 tttaggtagt tcgatatcaa tatctttcaa gttatgcgca cgagcacctt ttactactat
     3121 ggatggttct ttcatttact tgtcaccctt ctgcttttaa ttcaaataac atatctctta
     3181 attctgtagc tttctcgaaa tctaaatctt tcgctgcttg tttcatttct ttttctatat
     3241 tgtcgattgt cttttgacgt tcttttttcg tcatcttctt aggtatcaca gtttgtgctt
     3301 tgtcattatt ttcgtcattt tcaacagtag cactaattaa atcatgtatt tttttattaa
     3361 ttgttttagg tgtaatacca tgtttttcat tatgtttcat ctgtatttct cgacgacgtt
     3421 gtgtctcatc aattgcatac ttcatcgaat cagtcatttt atcggcatac ataatgactt
     3481 cacctttatc gttacgcgca gctctaccta ttgtttgaat taatgagcgg ttagaacgta
     3541 aaaacccttc tttatctgca tctaatatga caactagaga aacttctggt atatcaatac
     3601 cctctcttaa taaattaata cctacgataa catcatatgt acccattcgt aagtctctaa
     3661 ttatttcaat tcgttctaat gtcttgattt ctgaatgcag ataattaact ttaatacccg
     3721 cttctttcat gtatgtggtt aaatcttcac tcatcttttt agtgagcgtt gtaacaagta
     3781 cgcgttcatt acgctcaact cttgtttgaa tttcacttaa taaatcgtca atttgatttt
     3841 cagtaggtct aacctcaatc ttaggatcca gtaaaccagt aggacgaata atttgttcaa
     3901 ccatcttatc cgtatgttca atttcgtatg gtccaggcgt tgcagataca tacacaagtt
     3961 gttttgtctt ttcttcaaat tcttcaaatt taagtggacg gttatctaat gcactcggta
     4021 atctaaaccc atgatccacc aaaacttgtt tacgcgctct gtctccgtta tacatgcctc
     4081 gaacttgcgg taatgtcaca tgtgattcat caatcattac taaccaatca tcgccaaagt
     4141 aatccaataa agtatatggt gtcgaaccca gtggtcgcaa agttaaatgt acggaatagt
     4201 tttcaattcc tgaacagaat cccatctctc gcatcatttc taaatcatag ttggtacgct
     4261 gttctaacct ttgcgcttct agtaatttat tctcatctcg taattctttc aatcgttctt
     4321 ccaattcttt ttcaatacgt tcaatcgcaa ctttcaactt ttcttcacgt gttacgaagt
     4381 gagaagctgg gaatatcgca aaatgttctc tttctttcaa cacttcacct gttaggtagt
     4441 taacttctcg gatacggtca atctcatcgc cgaaaaactc aacccttata caaagttctt
     4501 ctttagaggc tgggaatatt tccactacat caccacgcac tcgaaacgtt cctcgttgga
     4561 aatcgatgtc atttcgtgta tattgcacat ctacaagttt tctaagtaat tcacttctat
     4621 ccatttccat accaactcga acacttacta ctaaatcttt atattcttca ggattaccta
     4681 aaccatatat acaacttaca ctagcaataa taattacatc atcgcgttca aataatgcac
     4741 ttgtagcaga atgtcgtagt tgatcaattt catcattgat tgaggcatct ttttcaataa
     4801 aagtgtcagt agacggtacg tatgcctctg gttgataata atcatagtaa cttacaaagt
     4861 attccaccct gttttcagga aaaaattctt taaactcact atataattgt cctgctaatg
     4921 ttttgttatg cgcgataatt aacgttggtt tcccaacttc tttaataaca ttactcatcg
     4981 taaatgtttt ccctgtgcca gtagcaccta ataaagtttg atgtcttttc cccgctttaa
     5041 taccttccac gatttcttta attgcttgcg gttggtcacc ttgaggctca aaatcagaat
     5101 gtattttaaa aggataatgt tcaaccattg tcacatatgc ctcctctttc aacgtattat
     5161 ttatatttcc gctcaactct ttgctttctc attacatatt ttagcaaagt agtcacacaa
     5221 aaagcaaacg tttgttcgta aaaatgtcga acaaagaaaa acaaacatag ttgtgctaac
     5281 tatgcttgcc ttaaatcaaa tcgtttatct tgttattttt agtatgtaaa tacacattat
     5341 attaatcatc attttgtttc aacaaatttg tttgaaacat tattttaaag ttaatcttag
     5401 cgatcttcat cttgatgttt atgaaattcg agttgatcta taattaaata accagctaat
     5461 aatgacacta catcaataaa aataatccac tcgttatgga aatactcttt atagattgag
     5521 gcaccaatta aaattaatgt cagaatagta ccgacccatt tacttcttgt tattacacta
     5581 aataatacta ccaagacaca tggaaagaat gctgcgctaa aataccatat cattcatttt
     5641 cctcttttct tttatttaaa atgttcatgg ttgtttctct taattctgtt ctaggtataa
     5701 agttttcagt caacatttct ggaatgatat tattaataaa atcttgtaca gatgctaaat
     5761 ggtcaaattg aataattgtt tctagactca tttcataaat ttcgaaaaat aattcttcgg
     5821 gattacgttt ttgtatttct ccaaatgttt cataaagcaa atcaatttta tcagcaactg
     5881 aaagtatttg gccttctaat gaatcatctt taccttcttg cagtcgttgc ttataaacat
     5941 ctctatattg taatggaatt tcttcttcaa taaaggtctc taccatttct tcttcaactt
     6001 gcgaaaataa tttttttaat tcactactcg catatttaac aggtgttttt atatcaccag
     6061 taaacacttc ggcgaaatca tgatttaatg ctttttcata taagcttttc caattaatct
     6121 ttcgtccatg atattcttca actgttgcta gatattgtgc aattttagtt actttaaagg
     6181 agtgtgctgc aacattgtgt tcaaaatatt taaattttcc aggtaatctt ataagtcttt
     6241 ccatatctga taatctttta aaatattgat gtacacccat ttcaattacc tcctccatta
     6301 attaatcata aattatactt tctttttaca tatcaatcaa ttaaatatca tttaaatatc
     6361 ttctttatat aactctgatt aaatgatacc aaaaaatcct ctcaacctgt tacttaaaca
     6421 ggctaagagg atagtcttgt cttgatatat tacttagtgg atgtaattat attttcctgg
     6481 atttaaaatt gttcttgaag atttaacatt aaatccagca tagttcattt cagaaacagt
     6541 aattgttcca ttagggttta cagattcaac aacaccaaca tgtccatatg gaccagcagc
     6601 tgtttggaaa atagcgccaa cttctggtgt tttatctact ttaaatcctg caacttttgc
     6661 tgcgtaattc cagttatttg cattgcccca taaacttcct atacttctac ctaattgtgc
     6721 acgacgatcg aaagcataat atgtgcagtt tccataagca tataagtttc ctctgttagc
     6781 aactgattta ttgtagttat gtgcaacagg tacagttggt actgattttt gtacttgagc
     6841 aggtttgtat gctacattaa ctgtcttagt tactgcttgc ttaggtgctt gcttaactac
     6901 tactttttta gatgcttgtt gtacaggttg ttttactacc tttttagctt ggcttgcttt
     6961 tcttactggt gatttaaccg ctttagtttg tttcacttta ttttgaggca caagtgaaat
     7021 cacgtcacca ggaaaaatta aaggtgttac accaggattg tatgaatata attgattcaa
     7081 cgttaagtga tgctctaaag caatcttata taatgaatcg ccagcaacta ctgtataagt
     7141 tgtcggtgat tgcgtttgtg cttgaacatt tgatacataa ttatgttgaa caggtgtttt
     7201 tacttgtgtg ccatgttgtt gtgcatgtgc tgcattattt aaagctaaaa aagctaacac
     7261 tgacgaaacc gtcactgtaa gagatttttt catcttgctg tcattccttt gctgttagta
     7321 ttttaagtat gcaaatacta tagcacaata cattttgtca aaagctattg ttataacgat
     7381 gtaatcaaat ggttaacaat ataaaaagaa tacaaccttt tatcatagtg taaaatgtat
     7441 tcataccatg taattgagaa cgttttcaat aattaattca ataccttgaa aatcgccata
     7501 ggtaatatta ctaaatgcac actgcatatg ttgttttaac aaacacaact tttaaaaaat
     7561 atattctaac tctatctacc gaattgtact taaatattca taaacaaatc atattccaaa
     7621 atctaattta caatttattt agctaccttt aaaaaaacca aaaaccgacg cccttttaga
     7681 gcctcggttt taaaatatat attaatcgtg cgacattgtc tgtcttaaat atgattcgat
     7741 aaatggtcca atgtctccat ccatcactgc atcaacctta cctgtttctt cgttcgtacg
     7801 atgatctttc accattgagt atggatggaa aacatatgat ctaatttggc ttccccagcc
     7861 gatttctttt tgttcgccac gaatttcagc catttcacgt gcctgctctt ccaattttaa
     7921 ttgatataat ttagacttta acattttcat agctgcttca cggtttttaa tttgagaacg
     7981 ttcattttgg ttattaacaa ctatacctga ggggtggtgg gtaattcgta ttgccgattc
     8041 agttttgtta atatgctgac cacctgcacc agaagctctg aatgtatcaa ctgtaatatc
     8101 atccggattg atttcaatct ctatttcatc attattaaaa tctggaataa cgtcgcatga
     8161 tgcaaatgat gtatgacgac gtcctgatga atcaaatgga gaaattcgta ctagtcggtg
     8221 tacacctttt tcagctttta aataaccata agcattatgc cctttgatga gcaatgttac
     8281 acttttaatc cccgcttcat ccccaggtag ataatcaaca gtttcaactt taaagccttt
     8341 cttctcacaa taacgttgat acattctaaa tagcatatta gcccaatctt gagactccgt
     8401 gccacctgca ccaggatgta actctagaat tgcgttattg gcatcgtgag gcccatctaa
     8461 taataattgc aattcgtatt catccacttt agccttaaaa ttaatgacct cttgctctaa
     8521 gtcttctttc atttcttcat caaattcttc ttgtaataaa tcccaagtag catccatgtc
     8581 atctacttct gcttgtagtg ttttataacc attaactatt gcttttaacg cattattttt
     8641 atctataata tcttgcgctt tcgtttggtt atcccaaaaa ttaggttctg ccatcatttc
     8701 ttcatattct tgaatattag tttctttgtt ctctaagtca aagagacccc ctaatttgtg
     8761 ttaaatcttg attatactta tctatatttc gtttgatttc tgataattcc atagcattcg
     8821 ctcctattta tatttcaatt caagtcattg atttgcatct tttataatgc taaattttaa
     8881 cataattttg ttaaataaca atgttaagaa atataagcac actgacaatt agtttatgca
     8941 tttattgtta aaaatcagta catttatcat cgacatatgc ctaaaccgat tttttaaaac
     9001 taagtacata acaacgttta acaacttctt cacatttttt aaagtattta acgcttgtaa
     9061 aataaaaaga ctcctcccat aacacaaact ataggtgttt aattggaagg agttatttta
     9121 tatcatttat tttccatggc aatttttgaa ttttttacca ctaccacatg gacaatcatc
     9181 gttacgacca acttgatcgc ctttaacgat tggtttcggt ttcacttttt ctttaccatc
     9241 ttcagctgaa acgtgcttcg cttcaccaaa ctctgttgtt ttttcacgtt caatattatc
     9301 ttcaacttgt actacagatt ttaaaatgaa tttacaagta tcttcttcaa tattttgcat
     9361 catgatatca aataattcat gaccttcatt ttgatagtca cgtaatggat tttgttgtgc
     9421 ataagaacgt aagtgaatac cttgacgtaa ttgatccatt gtgtcgatat gatcagtcca
     9481 atggctatca atagaacgaa gtaaaatcat acgctcaaac tcattcattt gttcttctaa
     9541 gatatctttt tgactttgat atgctgcttc aatcttagcc caaacgactt cgaaaatatc
     9601 ttcagcatct ttacctttga tatcatcctc tgtaatgtca ccttcttgta agaagatgtc
     9661 attaatgtag tcgatgaatg gttgatattc aggctcgtca tctgctgtat taatatagta
     9721 attgatacta cgttgtaacg ttgaacgtag cattgcatct acaacttgag agctgtcttc
     9781 ttcatcaata atactatttc tttcgttata gataatttca cgttgtttac gtaatacttc
     9841 atcgtattct aagatacgtt tacgcgcgtc gaagttatta ccttctacac gtttttgtgc
     9901 tgattctaca gctcttgata ccatttttga ttcaattggt gtagagtcat ctaaacctag
     9961 tcggctcatc attttctgta aacgttcaga accaaaacga atcattaatt catcttgtaa
    10021 tgataaatag aagcgactat cccctttatc accttgacgt ccagaacgac cacgtaactg
    10081 gtcatcaata cgacgagatt catgtcgctc tgtacctatt actgctaaac cgcctaattc
    10141 ctctacgcct tcacctaatt tgatatctgt accacgacca gccatgttag tggcaatagt
    10201 aacggcacct ttttgtccag cgcctgcaac aatttcagct tcacgttcat gatttttcgc
    10261 atttaacaca tcatgacgga taccacgttt tttaagtaaa tttgaaatat attcagaagt
    10321 ctcaactgca acagtaccta atagcactgg ttgccctgcc ttgtgttttt caacaacatc
    10381 ttctactact gcatcaaatt tacctttttg gctaatgtaa attaaatcag acttatcgtt
    10441 acgttgcaca ggtttatttg tcggaatttg agttactgtc atgttataaa tatttctaaa
    10501 ttcttcttct tcagttttag ctgtacctgt catacccgca agtttattgt acattctgaa
    10561 atagttttgg aatgtaatag acgccatagt tttagattca ttttgaattt gaacgccttc
    10621 cttcgcttca atagcttggt gtaaaccttc cgagaaacga cggcctggca ttgtacgtcc
    10681 tgtaaattga tcgacaatta atacttcgcc atcaacaacc atatagtcta cgtcacgttg
    10741 taatgtaacg tgcgcacgta aagctgtgtt gatatgacta ataacatcaa cattttgtac
    10801 atcatataag ttttcaactt tgaacatacg ttcagcttta tccgcacctt gttctgttaa
    10861 atgtacagct ttcgtttttt catcgtattt ataatcttcg tcctgtttta acattttcgc
    10921 aaaaacattt gcttgtgtat aaagtgacgt tgacttttca gcttcaccag aaataattaa
    10981 tggcgtacgt gcctcgtcga ttaaaattga gtcaacctca tcaatgattg caaaatgtaa
    11041 tggacgcatt actctatctt cagaataatt caccatgtta tctcgtaagt aatcaaaacc
    11101 tagctcatta ttagtactgt aagtaatgtc ttgtgcgtat gcttcacgtt tttcttctgt
    11161 cgtcttactg tttaagttta atccgacagt caaacctaag aagttatata actcagccat
    11221 ttcttcactt tgaacacttg ataagtattc attgactgta ataacgtgaa cacctctacc
    11281 agctaatgca tttaagtatg ttggcattgt cgctgttaat gttttacctt cacctgttct
    11341 catctcagcg atatcacctt tatgaattgc aataccaccc ataatttgaa ctttatatgg
    11401 tgtcatattg aatacacgtt tagagccttc tctaacaagt gcatatgctt ctggtaaaat
    11461 tttatctaaa taatcatttt gctttttgac attatcaatg tcagctaatt ctgtttggaa
    11521 ttgtttcgtt ttattacgaa tttcttcatc agttaaaatt gccgtttttt cttctaaagc
    11581 gattacttta tcagcaagtt tacctaactg tttaatttct ttattattgc catcaagaat
    11641 ttttgataaa aatcccattt cgttcgctcc tttagctaaa aaactgtttg gcctacaaca
    11701 atatatctta tcatttatag ttagaaaatt atacttattt actcatttgt agaatcaata
    11761 taaatatatt tatgacatac ttcattcaca ttctgttgtc aacaagttta tcactaataa
    11821 atatattctc aatacgcaat tatacttcct aataaattat attataaata ttttacgatt
    11881 ttcgactcgg actatacaat agactgacat actattatta acttaacatt caaatatata
    11941 catccgttaa cattagcata gtcactatgt ttcattcaac aaattacatt atcgaactat
    12001 gaaatagtca taatttgctt ttggagtata aaaaagcact tgtgcaaaaa cacaagtgct
    12061 ttaaacttaa tttattgttc actagtttga atcaagccat atttaccgtc tttacggcgg
    12121 taaacgatac ttgttccatc agtttctctg tctgtgaata caaagaagtc atgacctaat
    12181 agattcattt gtaataccgc ttcttctgaa tccattggtt ttaagctgaa ttcttttgaa
    12241 cgaataattt ctatctcgtt atcatcgtaa gcgtcattat caacttgtgt ttcttgcatt
    12301 tcttgtaatt cggcaacaaa cacttcttga tctcctcgat cacggctctt acgattaata
    12361 cgtgttttat attttcgaac ttgtctttca agtttattat taattaaatc aatacctgcg
    12421 tataaatcat cgtttcgctc ttcagctctt aacgtaacat ttttcaatgg aattgttact
    12481 tcaattttag tagctgaatt tgaataagtt ttaactttaa catgcgccac tgcatttggt
    12541 acgtcattaa aataacgttc caacttacca attttttcct caatatagtt gcgaatagca
    12601 tctgtgatag tgaggttatc tccatgaatt tcaaatctaa tcatagtaat ctctccttaa
    12661 acctctttat tggtaactct ttattatatt ttaacatttt tacgctatcg tgcaaacgca
    12721 aacactttga attttctgat atttttagca tataatttac accctgcacg atgaattgtt
    12781 aatccagttg tataaatatc gtcaacgagt aatatttcct taccatttaa atctaattcc
    12841 gtatcaataa taaatggatt ttcatctgcc agacgctctt tcttagttaa atgagactgt
    12901 tttggtctat ttgacatctt taaaatctta tcaaagcgaa tccctttagc ttttagtact
    12961 gcttctaccg ggttaaatgt tctagataaa tcatgtgccg gcgaagaagg aattggcaca
    13021 atatagtcat aagatgtttg tggtatttca atcaaatgtg ccaataattc acataaataa
    13081 tagtctttca aaaatttata ctgatgtatc atctctttca ttaaaccgtc atattgaaat
    13141 tgacaatata attgttccat taaattaaag tgtgccgata gaaacttgca gtctaaacaa
    13201 tacgcttcat cttgatttaa gtgttttaag caccttgaac atcgccttgc tttaatatca
    13261 agtttaatat tgtcccaatt ctctttgcat ctgtcacata atctattagg tttcttgaac
    13321 aaattataaa tggttatatt ttcatataac ttagcaccac aactcaaaca attattcatc
    13381 aatccaacct ctttttaatg ctaatttgtt catcctttga atctcttttt tagcttgaat
    13441 catattcata cttacccctt catgaaaaaa caatactttt ccagtaggac attctaattt
    13501 tcgtccaaca cgtccagcaa tttgtattaa agcctcttga gtatattgat gtgcatcgat
    13561 aacaacaaca tccaaatttg ccattgtaaa tccacgttct aatattgtcg tagtaaaaat
    13621 gacatcgaaa tgtccattcc ttaattgttc aactttttca aagcgaaaaa catcctcgct
    13681 atggacgtat gttaatttag taattttctg cttataaacc gaaaatgttt taatcattgt
    13741 ttctatattg ttaaaaaaca ccagtgtata acgttgatta ttaatttgat cttgtaaaat
    13801 tcggtataac attttctgaa tcttcttatt attaagtttg aaataacgat atttaggaac
    13861 tggaagtgat tttttatgaa agcgagctgg caatttaatt atattttcgt ggggaatctc
    13921 tgacagaagt tgtttcggtg gtgttgctgt catataaatt gttgcatgtt caactttaga
    13981 agatgacttc aatgcttgtt gtaaattttt atccattgat aaaggaaagg catcgacttc
    14041 atcaataaaa atagtatcaa agtgctgttt gaatcggtaa agttgatgca ctgtgcatac
    14101 aacaaaatgc ccttcaaatt gttgtcttga ttgctggtgt agtatgtcta tatcttcatt
    14161 aagaaatgcg tctttaatac gtttactaat ttctacaaca acatctacac gtggtgacac
    14221 aatagctata ttatctccct gtattcttgc atattgaatg ccttgaaaca tcatttctgt
    14281 cttacctgca cctgttacgg catataacaa aatcgtttgt ctctttctaa tggctcgaac
    14341 aatatgttct gaggcataag actgctgttc cgacaattca aatgagagat gataatatgc
    14401 ttgtgatgaa ctttgcgaac tctctgttat tttatatctt gttacattat ccattcgacc
    14461 cagtgatata caatttctgc aatatacaat tttcttggaa tgtactgagg aatcatactg
    14521 ataaaaatgc tttggatttg tactttcaca ttgaacacaa taatagtttg aattttgttt
    14581 gatgacacct gttacttctt ttttaatttt ttcattagta agtgaagtat gaggatgaat
    14641 taatttcccg taatgtttaa tgcttaaaac ctcatttcaa cgtaaaaaag agcctaatat
    14701 tataatgatt ttcttatatc tttagcattt tggctttcaa atgatgaaca tttaaagcca
    14761 tgtatacgag agataatttc caattacaat atagactctt ttattaaaat tttataatta
    14821 tgttaatctt atttttctgc caacatagcc taaacctaat ccaccagaac ctaaatgtgc
    14881 tgcaacaact ggaccaaact cagagtatgc tacttgataa gctgaaggac aatcatcttg
    14941 taactttttg tataacgctt gaccatcttc gaaatgatct ccatttatga caaataaagt
    15001 tacttcttca aagtctttta caatatctaa tactttcttt tctaatgttt gaatggcacg
    15061 ctttttagta cgaacttttt cttctggtat aatcttgcca tcttcaaact taagaactgg
    15121 cttcattttc aataatgttc caacccatgc ttgagcacca gtaattcgac cacttttttg
    15181 taaattcttt aagtcatcaa caattaaata tgcacctgtg tgttcacgca tattagttaa
    15241 atcatcaata atttgctgtg gctcgtatcc ttcttcaacc atttcaatag cacgtaatac
    15301 ataacatcct tcaatcattg ctgcaagctt actatcaaat gcatgtacat taacaccttc
    15361 aaccatttcc cctgcttgat aactagattg ataacttcca ctaatcccac ttgataagca
    15421 aatgacaatg atatctgtgt aaccttgatc tcttaacatt tcatatttag taatccattc
    15481 gccaattgct ggttggcttg ttgttggaat cgtttgagac gatgccattt tattataaaa
    15541 ttcttcaatt gctatttcat tactttctgt aaagttcttc ccatcatcaa aagtcacact
    15601 taatggcgct atttgaatat tatatttatc gattaagtcc tgcgacagat aacttgtaga
    15661 atcggtcatc acagcaattt tcatagtcgt cctcctaaaa tatactcata gcaatgatac
    15721 aatatttcag ttaaaaagaa aatggcaatc aaacgattta tagtcatgtc attttcagtg
    15781 tataatgaag attaaataat cttaagctta tgaattaagg agttattatg acacaaaata
    15841 tcattactgt taaaaaagaa cacattattg aaaatgtgat tagtaaatca agattcattg
    15901 ctcatatcaa acctgttcaa aatgaagatg aagctaaagc atttattgct gcaataaaaa
    15961 aagaacataa agatgccact cataattgtt ctgcatatac tattggacct gaaatgaata
    16021 ttcaaaaagc gaatgatgat ggtgaaccga caggaactgc cggtgtccca atgttagaca
    16081 tattaaaaaa gctcgatgtt cataatgcgt gtgtcgtagt aacaagatat tttggtggta
    16141 ttaaattagg tggtggcggt ttaattcgcg cctatagcgg tgctgttaga gacgtcattt
    16201 atgatatcgg gcgtgtcgaa ctacgagaag cagtaccatg tatagtaaca ctgaattatg
    16261 atcaaactgg taaatttgaa tatgaattag cttcaactca ttttatgtta agaaaccaaa
    16321 cttacactga taaggtaagc tactatatcg atgtcgttaa aagtgactat gaagcgttta
    16381 ttaacttttt aaatcaaatg actgcaggaa atttcgattt gactgaagaa actccaaagc
    16441 agttaccttt cgatatacca actgaataaa aataaaaagc actatcataa gacggtaaat
    16501 atcaattgcg tattattaaa cgacaattga agtacacgtt tatggcagtg ctttttatat
    16561 ttgaaattta atacaaaaaa attgtaaaga gtaaagccat ataatacaaa ttatcccata
    16621 cagctatgct ttcattccct attcctcttt atgagatgac ttacgactaa ttaaatttaa
    16681 tattggtcgg tagttgttat ctattaatcc tgtaaattca acaattaatt caatactaaa
    16741 tatgattaat acaaacatta atacaacacc taatggtggc gatacataca aaataatgct
    16801 cgaaagacta aataaaatag agattgaata gattaataat actgtttgtc tatgtgtgta
    16861 gcctaaagct aatagtttat gatgcaaatg cgatttatca gcttgcatta tatgctgccc
    16921 ttttttcaca cgtcgaatca ttgcgaacaa agtatcaatg aatggaactg ctaagataac
    16981 aattgggaag aacaatgcaa taattgtaat atttttgaat ccgagtaaag aaaggaatcc
    17041 gatgataaat ccaatcatta aagccccact atcacctaaa aatattttgg caggatggaa
    17101 attgtaaaat aaaaacccaa ttaaagagcc taataaaaca caacaaatca tcgttatgaa
    17161 aatattagct tgtaaaattg caatgaaccc tattgtaatg agtccgattg cagaaacacc
    17221 cgacgccaaa ccatcgagtc catcaattaa gttaattgca tttgtaatag caacaatcca
    17281 aatcacagta attggaatac taagaaatcc aaaatgaatc gttgtaccca ttggcaacga
    17341 aataaaatca atagtcacac cataaaaagc aactactaag gcagcggcaa tttgaccagc
    17401 caattttata tacggtttca aatcgtagat atcatctaca agcccaagta cgtacataat
    17461 aatcgcacca ataataagtg gtttgatttc tgtttcaata ggatgaccaa tccaaatacc
    17521 tattaaaaat gaaaagagaa tcactgtacc acccataact gaaataggtt tagtgtgtac
    17581 ttttctaaaa tttggtttat caactaaatt taatcttttc gatattgcaa taacaattgg
    17641 tgttatcgtc aaactgacaa tcattgttac tgcaactagt aataatgtaa ccatttattc
    17701 accttcatcg atattaattg tttttaacgt tatatttatt taatattgtc tttatagaat
    17761 gatatatcta ttttctacgt atatcttata gctttattat aaattaattc gttcaatcaa
    17821 tgatatcata tttacaacta tacgattcat ttcttatata tcgcctatta ttcatttttt
    17881 catcatatca tttaaggcaa cattttaaca tcaaagtcta tatcatacat taattttata
    17941 tgtaaactaa tgacaaattg taagttttca cttacaatag ctagtggtca attaaaggag
    18001 tcgattcaga catgttcgaa gcatttatat acaatatatc tgttatagtc gctggaatct
    18061 atttattcca tcgcttacag tattcagaaa acaaacgtat ggttttttct aaggcttatg
    18121 tcactgtttt aatgacaatc gtatcattat tattatctgt ctaccctatc ccttatcgtg
    18181 aagattattt aattcactta acattcgttc ctttattgtt tttaggtaga tttacaaata
    18241 tggtctatac attatctgcc acagtaattg tagcaattgt tgaaattgta gtatttaaca
    18301 actcgattat gtacggtgtt acgttgattg ttattgctgc agtaacaagt gctattggac
    18361 catttttaaa acaaaatgat gtgctatcat tattaatact taatgttgtt acaataatca
    18421 ttttatttgg tgtcgcgtta gtgagtccta tttacacact atcggaagtt atcattttaa
    18481 taccaatttc attaattatc actttagctt cagcgataac atttgtcgat atttggcatt
    18541 tcttctcact cgtaaatcgt tatgaaaacg aagataaata tgattactta acaggcttag
    18601 gtaatgtaaa agaatttgat agacatttaa atgaaatttc acgaaaagct gaaaaagaac
    18661 atcaaagtat cgcattatta ttaatcgata tcgatggatt taaagatgtc aatgatacct
    18721 actcacacaa atcaggtgat gctgtattaa aacaaatgtc tcaattactt aaaaactatg
    18781 tgccgaatca atttaaaatt tttagaaatg gtggcgaaga gttctcagtt gtcattcaca
    18841 attattcact tgatcaaagt gttaaattag ctgaaaatat tcgttcgggt gttgaaaaat
    18901 catcattcca tttaccaaac aaagaagtta ttaaattgtc agtgtctatc ggtgttggtt
    18961 atttaacaga tgacgatcca aaatcacagc gtaaagtgtt taaagatgca gacgatatgg
    19021 tgcatgtagc caaaaatcaa gggcgaaaca aagtaatgtt taaccctatt atcaatttat
    19081 aacaacgaca aaactgttaa tcctttgcta ttattaaatc atagtataat agcaaagatt
    19141 aacagttttt cttatttaaa tttgaaccat acaagttcat catactaaca caaagtctca
    19201 ctattgaaag ttgtaacaac aagttgtaaa cttcaattgt tcaaataaat aacacatatc
    19261 attggaggtg aattgatatg ccagattcaa tcacaattat agatgaaaac aaagtgattg
    19321 atgttgtatt aattgcaggt agaattttac ttgaatcagg tgctgaaaca tatcgagttg
    19381 aagatacaat gaaccgtatc gcacatagtt atggtcttca taatacatat agttttgtca
    19441 gttcaactgc aattattttt tcattaaacg atcgaacaag tacaagatta attcgtgtac
    19501 aagagcgtac aacagattta gagaaaatcg ctttaacgaa tagtctttca cgtaaaatat
    19561 caaataaaga actcacaatt gatgaagcta aatctgaatt tattcattta cagcatgcat
    19621 cattacaata ttctttttta acgaatttct ttgcagctgc cattgcatgt ggcttcttcc
    19681 tatttatgtt tggtggtgtc gcatcagatt gttggattgc agtcattgct ggcggatccg
    19741 catttttaac attcagcttt gtgcaacgct atatacaaat taaatttttc tcagagtttg
    19801 ttgcagccgc tgtggtcata tccattgcag ccacatttac taaattaggc attgcaacca
    19861 atcaagatat cattactata gccagtgtca tgccacttgt ccctggtata ttaattacca
    19921 atgccattcg agatttatta gcgggtgaat tacttgcagg tatgtctcgc ggtgttgaag
    19981 ctgcattaac agcattcgca atcggtgctg gtgtcgcaat cgttttatta atcatttaag
    20041 gaaggactgt taaagcatgc tattttattt atttcatttt acaatcagct ttatatcaac
    20101 agtacttttc tctatcattt tcaatgcacc caaacgcctc ttagtagcat gtggatttgt
    20161 gggtgccatt gcatggacga tttaccaatt aacggtagat ttagagtttg gaaaagttgg
    20221 cgcttcattt ttgggaagct taattttagg cttaatgagt catactatga gtcgcagata
    20281 taaacgaccg gtaattatat tcatagtgcc aggcattata ccattagtac ctggtggtgc
    20341 agcttatcaa gcgactcgtt ttttagtatc aaatgattat acaagtgctg taaatacatt
    20401 tttagaagtt acactgattt caggtgcgat tgctttcggt atattagttt ctgaaattct
    20461 atattaccta tacacacgta tcaaacaact gtatggtaaa atcaaaggta agacatataa
    20521 aaaatcttac aacatgaata atagagttta aggggtcgaa tataatgaag aaccaattga
    20581 tagatagatt aacaagatat acgacaattg atacgcaatc tgatccaaaa tctacaacaa
    20641 caccttcaac tgaaaagcaa tgggatttgt tacatttatt agaaaaagaa ttacaacaat
    20701 taggattgcc aactgattta gatgaaaatg gctacttatt tgctacatta gaaagtaaca
    20761 tcgatgtcga cgtcccaaca gttggatttt tggcgcatgt tgatacatca cctgatttca
    20821 atgcttcaaa tgttaaaccg caaattattg aaaactatga tggtaagcca tacaaactag
    20881 gcaatactaa acgtgtctta gatcctaaag tatttccaga acttaacagt ttagttggtc
    20941 atacattaat ggtaactgat ggtacatcgt tactaggtgc agacgataaa gcgggtattg
    21001 tggaaattat ggaagctatt tgttatttac aagaacatcc agaaattaaa catggtacca
    21061 ttcgcattgg atttacacca gacgaagaaa tcggtcgtgg tccacataaa tttgatgttg
    21121 accgcttcaa tgctgatttt gcttatacta tggatggtag tcaatatgga gaattacaat
    21181 atgaaagctt taacgctgct gaagcggtta ttacatgcca cggtgtaaat gttcatcctg
    21241 gttcagctaa aaatgcaatg gtaaacgcaa tacgtttagg tgaacaattc gatagtttgc
    21301 tacctgatag tgaagttccg gagcgaacag aaggatacga aggcttttat cacttaatga
    21361 actttgaagg aactgttgaa aaagcaactt tgcaatacat tattcgtgat catgataaaa
    21421 aacaattcga attgcgtaag aaacgtattt tagaaatacg tgacgatatc aatgcccatt
    21481 ttgaaaatta tccagttaaa gttgatatat cggatcaata tttcaatatg gcagaaaaaa
    21541 tattaccatt gcctcatatt attgatatac ctaaacgtgt ctttgccaaa ttagatattc
    21601 cagcaaacac tgaacctatt cgcggtggta cagatggttc acaattgtca tttatggggt
    21661 taccaactcc aaacatcttt acaggttgcg gtaacttcca cggcccatat gaatatgcat
    21721 ccattgatgt tatggaaaaa gcagtacaag taatcattgg aattgtagaa gatatcgctg
    21781 aaaatcacta aaaaccaacg attatatact agttcaactc aggcttaatt aatatataaa
    21841 aatcacgcta acattcagtg caatgatagc gtgatttttc atatttttta cgtatacttt
    21901 cgatcttcta attcgttttg ctaaccatca taaatgttat taaccatcat gacagcatca
    21961 ccaatttagt ccttctttac taaacctcaa cattaaaatg tgttttcaac agttttaaag
    22021 actgaaccgt ataatgccta atttgtaacc ccattttaaa gtcagtataa gtttctggca
    22081 tatcgataaa tgtattaaac attgctgtaa ccccttgtga ttcaaataaa tcaaactttt
    22141 cagcagttgc acaaattgca atggcaacct tttgatgttt atgacaaagt tctgcaatac
    22201 gcaatgttgt cgtttctagc aactgatcat tttcatttaa tccttctcca aaaataatta
    22261 aatccgcttg ttcaactaaa ttttctaaat gtgttagttg gtctactaat gcatgactgg
    22321 ttaatatttc agcttgatac agtccattca agactgctgc aattccacca ccagcaccac
    22381 cacgttcaac tggaccaatt gcaattttca attcactttt aaataactca ctaaaatacc
    22441 aaattaaatt atcgatttct gctgcttgat tatgattcaa ctgatacgca tcataagttt
    22501 gcatgatttc actttgctta ccatataatc gacttgaaaa atccgacatt acttgaattc
    22561 ttgctgtttc cattttaggg tgtaagttcg acatatccat acgacgaata tatttaatta
    22621 caccagcacc ttgtctcata tctacgacac gcccttcgtc atcatagaat tgagcaccta
    22681 atgcttgtaa catacctgca ccagcatcaa aactatcaat cccacctagt gaaattacaa
    22741 catgttttgc gtcgttatct aatgcatgtt taatcatttc tcctaaacca taacttgttc
    22801 gttcaacaat tggtttttta ccttttaaaa ataaattccc ctcgataacg gtcatccctg
    22861 tatcagtttg tccgtaaaca ccttcaactt cattcatatc tgcatcatgt actggtatac
    22921 gatacttttg cccagattgc cataaaaata cagaatctaa taattcatgt cttccattaa
    22981 acaatggtac ttgaactaca tctgcagttt caatttggct tgcaactgcc tcttcaacat
    23041 atctattagc ttgataactt gaaataattc catgaaactc atccatggct actaatactt
    23101 tcataacctt gaccacctta ctttattatt tgttgtgata cgttttttat tcataaaaga
    23161 ttaatcttaa attttagatt ttatctcgtg attatatgcg aacatttaac atgacatgat
    23221 tattaattat tccagtaaat aacaatatgc ttcttttggt ttagcatata tttcctatta
    23281 aactaactta caaatcatgt ttaaattttc ttattggatt cgtcgttttc caaaatattt
    23341 tcaataaaaa tactcgaata tattatacat atgtttgtaa aaaagtacta acttaactgc
    23401 ttctaaaggt gacataaaat aaaaggtcag aacatcatta tagccaaact actctgattc
    23461 acacgaattt aagttgtgct attcacgtct agtagtgtaa ggcaaaatgt tccaacccta
    23521 tagtttcatt attcttctgc ttgtgctaat gacgacacat tgatatcacc gtggtctcga
    23581 ttccaaacca tttcaccatt tacaaaataa aatgcttgag gtgattcatg tttaacgttc
    23641 gtttttttag caatataatc tgacaaatcg cgttcttgtt ggacaatcaa ataataaccg
    23701 tccatatcgc gttcatataa aaatttatta aattgatcgt acgcatttgc cgatattgga
    23761 caagtttcac tatgttttaa tacaaaaaca tatttatttt cctcaataac ctgttcaaat
    23821 tggtcaattg aacttagctt tatagccact ctattcacct ctaaaaatat ttatcacatt
    23881 taaatagcat ctgcgtgaca tttttataca tatagttaat atcaatgttt accattatac
    23941 acatagtaag ttgacatgtt aaccataaat tcaaaatttt taactttgaa aaatgattat
    24001 agttattttt cttctaattt ttcaagcttt ttttcaaagg ctttatcgcc gtgagttata
    24061 tctataccct ttttcggcaa cttatctaca tcgacttttg ataacttctc actaaccttt
    24121 attgtttcaa tacgtgtatt ataatagttc tgcagactgt aatacatttc tattgcgttt
    24181 ttccttgcat tattaacatg cttatcacta atattagttt ggttaatatc ggtaatttgc
    24241 ttttcaatca ttggcataat gtgattttta atcgcgcctt ttacttcatt ttctttacta
    24301 tcatctaggt tcttcttcgc agtatctctt aacgctttat tattatgttc taattttgtc
    24361 gtaagattag ttgcttcact tttattatca gctaatttaa tttctgattc aactttgtat
    24421 ctattttttt caaattgttt cgtataatct aaaatgtctt cgttatactt gatagattga
    24481 ttacataaac ctatgaattt ttttaattga tatatcgcat ccttcttctc atttgcaagc
    24541 gtcatatatt ctttttttaa tttcttaact ttcattgtat catcaggcaa attttttgct
    24601 gacttataat atgcttcaaa tttaggtatg agatggtttt taacatcttc ttgtagtgcc
    24661 ttaaattctt tactattttt atcagttgta tcagttttac ttagatgatc aatttctttc
    24721 aaatgtatat tatccatgac atgttgtaat tctttttgtt tttgctcaac atctttaaat
    24781 tgcttagtaa attgatgttg tgcctctttt tctttattac cacaagcact tattactaca
    24841 aataaaatcg ctaaaacagc gataataaca atttttttca tgattgcact ccttaacaga
    24901 ttcattatac aaaaacatgt ttattatgaa aatgaattat gttatagtat tccagacttt
    24961 atcgtgatta aattaatttt caatatcaaa ggagtttatc atgtattcaa atgttcaacc
    25021 attcattaat agtgatttaa ctaattgttt tcaatcacaa tacactgaaa ttgaatcatt
    25081 attattcaac ctcttagaca gcccagtaaa atatacaaaa catattggcg gtacgcatca
    25141 ttttaaatat tcaacagagc caattttaga tattttagta ggtgtagaaa atttgcatga
    25201 tattacatcc ttagatgaaa aacgcttaaa ttatgtaggg ttttatcgtt tgcatcataa
    25261 atatactaag aaagttatga tggcgaagtt taacaatttt catgaactaa aacaacaggt
    25321 gcgtctacat atcattcaaa tgaataccca aacatttgaa tcttatcttg cacttgatta
    25381 cctcttatca actaatgatg atattgcttt gcaattcaat acaaaaaaac accaaatatt
    25441 gcaacatagc ttaacaatta gacaatatga gatgcaaaag caacaatact ttgaaaaact
    25501 atgtaaaaaa tacttataac gacaataatg ccgtatttta taattatact aattctaata
    25561 ctttcttttc aattttcgca aatgaatttt aaaattggta taatactata tgatattaaa
    25621 gacatgagaa aggatgtact gagaagtgat aaataaagac atctatcaag ctttacaaca
    25681 acttatccca aatgaaaaaa ttaaagttga tgaaccttta aaacgataca cttatactaa
    25741 aacaggtggt aatgccgact tttacattac ccctactaaa aatgaagaag tacaagcagt
    25801 tgttaaatat gcctatcaaa atgagattcc tgttacatat ttaggaaatg gctcaaatat
    25861 tattatccgt gaaggtggta ttcgcggtat tgtaattagt ttattatcac tagatcatat
    25921 cgaagtatct gatgatgcga taatagccgg tagcggcgct gcaattattg atgtctcacg
    25981 tgttgctcgt gattacgcac ttactggcct tgaatttgca tgtggtattc caggttcaat
    26041 tggtggtgca gtgtatatga atgctggcgc ttatggtggc gaagttaaag attgtataga
    26101 ctatgcgctt tgcgtaaacg aacaaggctc gttaattaaa cttacaacaa aagaattaga
    26161 gttagattat cgtaatagca ttattcaaaa agaacactta gttgtattag aagctgcatt
    26221 tactttagct cctggtaaaa tgactgaaat acaagctaaa atggatgatt taacagaacg
    26281 tagagaatct aaacaacctt tagagtatcc ttcatgtggt agtgtattcc aaagaccgcc
    26341 tggtcatttt gcaggtaaat tgatacaaga ttctaatttg caaggtcacc gtattggcgg
    26401 cgttgaagtt tcaaccaaac acgctggttt tatggtaaat gtagacaatg gaactgctac
    26461 agattatgaa aaccttattc attatgtaca aaagaccgtc aaagaaaaat ttggcattga
    26521 attaaatcgt gaagttcgca ttattggtga acatccaaag gaatcgtaag ttaaggagct
    26581 ttgtctatgc ctaaagttta tggttcatta atcgatactg aaacacgctg tcgccattat
    26641 tttaccgaag aagatattat tgctattaaa tttaaatgtt gtaataaata ctatccatgc
    26701 tataagtgcc ataatgagtt tgaaaagcac gccattaagc gttggtctga gccttcattt
    26761 aacgaaaaag caatcttatg cggtgtatgc aaacacgaat taacaatcaa tgaatatatg
    26821 atggtagagc gttgtccaaa ttgccaatct cggtttaaca atcgctgtaa atatcactat
    26881 catatttatt ttgaaattta aacaacatag tcaaaacatt atagggagtg ggtcagacat
    26941 gataaagcac cacttctgta gaattccttt ttgaaattct ctatgtttgg gccccgccaa
    27001 cttgcattgt cagtagaaat tgaaaatcaa atttctctat gtttggagcc cactagtatt
    27061 gaaatgaact tgttacaagt acatgttcat tcaaccaacg actgacaaca gaacatttta
    27121 gagtctagga cattgattga cgtcccagat ttcctttttt aaagaaaaga ggggagcaat
    27181 gattaagacc tttaaccatt gttcccctct tttaaaatta ttttacaact ttatcaagtt
    27241 cctcaatttg tttaattgta gttgtagttg atccagctgc aaagtaccat aatttaggat
    27301 ctaaattata tactttgtct tctttaattg ctttaacatt ttttaataca ggattattta
    27361 atgcttgttt cgcagttgat ttaccactta tcgcttgacc tctatccatc gctaaaataa
    27421 catctggatt ttctttatta acatattcgt tagaaacatt ttgtccatga ttgctattac
    27481 ttactttttt atcaactgca ttgaatccta atgtatcgta aactaatcca ccaaaacgac
    27541 ctttaggtcc aaatgttgat aattcacctt cgttaactag taaatacata acagttttat
    27601 tgaagttttt cgttttatct ttcattgaag caattttgtt atctaaatct ttatttaatt
    27661 ctttagcttt atcttcttta tcgtaaattt ttccgatatt ttcagtgttt tgtttcattg
    27721 aaccaattaa gttcttttca tctgcaccaa cataaacaat tttcgcttta ggtgcagctt
    27781 ttttgaattc atctaaattc ttttgattag ctgtacgtcc agagataaag attacttcgg
    27841 gtttcgtcgc agcaatttta tcaaaattca cttcttttaa attaccaacg tttgtatatt
    27901 tatcatcttt aaatgattct aagaaattcg gtaatgactt accgccttcc cctttaggta
    27961 atgcttttac tttatctgat aagcccattt ctttcattac atctaatgcg ccatagtcta
    28021 acacaactgc atttttagga ttttttggta cttcaacagt ttctttaact tttttagcgt
    28081 cactaccatc ttttttctcg ccacgcattt tatagttatt ttcaattttt actgtatctt
    28141 tagaaccttt agtttctgat tttgattgtt ctttatcaga attgttaccg catgccgcta
    28201 ataaaaacat tactgctaat actaaatata agactgtttt cttcataata taatattctc
    28261 ctcttttggc ttaatctgtg tgcttgatgt gaaatataaa taaatgcttt acgtttaacc
    28321 cttctttaat tggcgcatca cccctttaaa gtgtctccaa attatcaatc cttaaaatat
    28381 aaacaaatcc tttgatttct tatatgttca atttgaatat tcatgtcata taaatcactt
    28441 aaaacactac tttgaatgac attatctttc aaatctgact taactagttg tccgtttttc
    28501 aatgctacaa tctgatctga ataacaggac gcaaagttaa tatcatgtaa cacaatgaca
    28561 atcgctttat tcattttatg cgctaacatt tttaacgttt gcataatttg aacagcatgc
    28621 ttcatatcta aattatttaa tggttcatct agcaaaatat attcagtatc ttgtgctatt
    28681 gtcattgcaa tgtatgcacg ctgacgttgt ccaccagata atgacttaat attacgattt
    28741 ctgatatctt gtagttgcaa caaatctagc gcatcattga caatatcatg atcctctttc
    28801 gtcaaacgac ctttagaata agggaatcgt ccaaagttta ccaactgctc taccgtaata
    28861 ttcatttcag tatggtttgt ttgttttaat atagatattt ttttcgacaa gtcattattt
    28921 ttataatcag acatgagctg tccatctatt ttcacttcac cgttctcaaa acgaattaac
    28981 ctacatatcg ctgaaagtaa agtactctta cccgcaccat taggtccaat taaagaagtc
    29041 aatttacctt tttcgatatc tatgctaata tcttccaata tcatttgatt atttatagtt
    29101 ttagttaaat tttcaacttg aatcattgcg catttctcct tctaactaat agataaatga
    29161 aataacttcc accaatcaaa tcaataagta tactcatttc tgtcgtagct tcaaacacat
    29221 tttcaactac ccattgcgca ctaaataaac taatccaact caagcaaatt gtcgcaatta
    29281 aaatatactt atgttcatac gtcttcatta gttcatgcgc tagatttaca gttaataaac
    29341 ctaaaaatgt aattggtcct actaatgcag ttgacacaga aactaaaact gagactatta
    29401 caagtagaat tcgcgttaat ttttcatacg atatcccaag attaattgct tcagcacgac
    29461 ctaaaagcaa tacatctaaa taaggcaata gtaaaattgt aatgactaat aagattacta
    29521 atagcactgc tgagaaagta actaaattcg aattagaagc attaaaatta gcaaacatac
    29581 tactttgtat tgctaaaaat gactcaggat ccataatcag ttgaataaaa cctgttatgc
    29641 ttctaaaaaa tgttcctaaa aggacaccaa taagtaagat aaaataaatt gaaaaatgtc
    29701 cgattttaaa gataccttgg aataggatta gtgcgaataa caccatcgtt ataagtgtta
    29761 atataaagtt caaatataca ttagtaaccc ataccgattg aattccaaat aaaaagactg
    29821 gcaatacttt gataaataaa taaactgcat ctaaccccat tattgatggt gtcaatagac
    29881 ggttatttgt aatcgcttga aaaatcacca ctgaagttgc aatggcagca cctactaaaa
    29941 taattaatat gaactttctt aaacgacttg aaaattgata ttcaaatatt tcaaaatcaa
    30001 tacctacaaa taagtacagc atactaatta ataacgttac tgcaattaaa agcattattt
    30061 ttttattatt gttgcgcata atttttccta cctttcataa gcaagataag gaaaatgatt
    30121 gttccaaata cacctattgt taaaccaata tttatttcat atggataaac aacaattctg
    30181 ccaactatat cagaaaataa tacaaagatg gcacctaaca tcatcgtatg agggatagca
    30241 tttttcaaat gatcacctcg ataaattgaa ataatatttg gtattactag tcctaagaac
    30301 ggtaatgttc caacagtcac cactaccaat gctgtaatag ttgcagtaat aaacaatgcg
    30361 atgttaatta atttttcgta actcaaacct aaattattag taaagtcttt acccattcct
    30421 acaatcgtga aatgattagc aaaaagatat gtcaatgcta aaagaggaat acttaaatat
    30481 aaaatttcat agcgtccact tgtgataatg gcaaagttcc cgttaagcca gttaccaatg
    30541 ctttgaacag cattcgtgcg caatgagatg aaggttgtga aacttgcaac aatcccaccc
    30601 atcataattc ctaaaagcgg tacaaaaatg acatctttca cttttataaa atcaatgatt
    30661 ttaacaaata aaaacgtacc gcaaatactg caaataacag caaatactag ttttaataaa
    30721 atatgaccgg ttggaaagaa caataaagca attaaaatac ctagtttagc ccattccatc
    30781 gttccagctg tagtcggact aacaaactta ttttgcatca tttgttgcat tatcaagcct
    30841 gctaaagcca acgaacttcc agcaattaaa atactcatcg tccttggtat tcggcttgag
    30901 aataaaatat tccgctgtga ctcagttaaa tgtagtaaat ctttaattga tagttcactc
    30961 acacctatga acaacgaaat aattgtcaac aatattagta atagaagcaa aatatttccc
    31021 tttaataaat acttcattac aaatttttct ccctttaaat taatgataat cattatcatt
    31081 gataatattt ctcattaata gtagcgtttt ttgaccataa tttcaataga ctttcaaata
    31141 atttagtgtc aattttattg atatttgcaa ttaaactgaa atttaatttt tcggaatgtg
    31201 tattttgttt acttaaagta aaatagaaca cttaagattt tgatgtctgg gaatagtgga
    31261 aatgataaaa actactaatg attgattaag tagtggttct taacattagc ctcagctaat
    31321 gtgtacttaa aaataggaat acatgagtaa aactcatgtg gaagtgataa tttctaaagg
    31381 aaaaagtttt tctttatgtt ggggccccgc ccgaacttgc acaatattgt tagctgactt
    31441 tccgcctgct tttatgttgg ggccccgcca acttgcacaa tattgttagc tgactttccg
    31501 cctgcttcta tgttggggcc ccgccaactt gcacaatatt gttagctgac tttccgcctg
    31561 cttctgtgtt ggggccccgc caactactgc caatataaat agcattgtac atcttagagc
    31621 attgatttct tactcagact cataatgagt tagatacaca taaaaaaaga ccttcaacat
    31681 gtaggagaca agacgaaacg aatcgctatt tccctttaca tgtgaaggtc ttttaattta
    31741 attgttattt gttgtcaaat acaaagtcat catcttgtaa tgcttctacg tttaatgcta
    31801 atacataacc atcacctttt actgagaaga agtcatggtt tttagttgtt gtatctaagg
    31861 cattctcaat gattgggtta aattcacgtt cctcaaaata aggttcaaag cctaagtttg
    31921 aaagtgcttt gtttccatta tatttaacat agtttagcac atcttcagtg attccaagat
    31981 catcgtataa catttttgtg tatgactctt catttaaata caagtcattt agcaatttat
    32041 acatttcttg atctgctttt tgtttctcac tttcagatag ttcatttcgt aaatgctgtg
    32101 catctaaacc ggtaaataca ccatgaatag attcatctaa aagaatttta cgaatgattt
    32161 cacctgatgt cgtcattttc ccttgaccag caagatatag tggataatag aaacctgaga
    32221 agaataagaa tgtttctaaa aatacactcg taactctggc catatattgg tcgtatatcg
    32281 aagcttcttt accccaaagt ttgtgataat tagcaacaat tttatcagat ttatatttta
    32341 aatggggttc ctctaaaacc cattcatcta ataggtagtt tgtttcacta gatggtaata
    32401 gtgttgtgaa aatatgtgaa tagcttttcg cgtgtatttg ctccatcatc gccataaatg
    32461 aataaactgc ttttttcctt aagtcagtcg tatgtagcat aactaaaggc atgccatcat
    32521 ctgcttgatg tgtatctaag cctgttaaac cagctaatgc ttttttaaat gtgtcttgtt
    32581 cagcttcaga taaagtcttc caacttgcaa tgtcttttga tactttaaat tctgtttcaa
    32641 cccacatttg agatatattt tgtctccaaa acatattcgt catatcttct tgtgtgttcc
    32701 aattaacagc tatcatgaat ataatttcct cctgtaatgt tactcaataa ttgacaaata
    32761 cagacatagc agtctaatca tctgctaatt cagagcagat gattagctgt ttgtcatttt
    32821 caacatttaa ttgttagata gaacaacttg tacattcttc tacacttaat aatttatttc
    32881 tagtatagta aagtgatttt aatcctttat agtgcgcata tacatataat cttgctaact
    32941 cacgtgtaga aatttcagaa ttaacataaa ggatcgttga gataccttgg tcaatatgcg
    33001 tttgaattgt cgcaattaaa tcgattaatt tcatctgatc agtattgaat gctgatttgt
    33061 agtaccacat tgtttgtggt gataagaatg gcatagggta aaatgtttcc gcattaccat
    33121 aagtacgacg ttcaatttgg tcaacgattg gcattacaga acttgttgca ttttgaacat
    33181 aagaaatact ttgtgttgga gcaattgcta atctatatgc atgatataaa ccatattgtt
    33241 caacatcttg ttgtagtttc ttccaatcct cagaagtagg aatagccata ccatcgaata
    33301 attcacgtac tttttcgaat tgaggttcaa attcttgagt tgtatagaac tcgaaatatt
    33361 tgccattagc ataatcagac ttttcaaagt cttgatattt gataccacgc tctttagcga
    33421 tttccattga acgttcgatt gagtagaaat tcatcatcat aaagaagata tttgcaaaat
    33481 cttttgcttc ttctgactca taaccaattt tattttttgc taggtaaccg tgtaaattca
    33541 tcacaccaag accaactgaa tgtaattcac tgttagcttt tctaactcct ggtgcatttt
    33601 gaatatttgc tacatcactc acaacagtta atgcgtccat accagagtga actgaatctc
    33661 tgaattttcc gctttccatt acattaacaa tatttaatga gcccaagtta caagaaatat
    33721 cacgtttaat ttcgtcttca ataccatagt cattaataat tgaagtttct tgtagttgga
    33781 aaatttccgt acataagtta ctcattttaa tttgtccaat gtttgaattc ggatgcactc
    33841 tgttagcatt atctttaaac attaaatatg gataacctga ttgtaattgt gtttgcgcaa
    33901 ttaaattcaa catttcacgc gcattctttt tctttttctc aacatttggg tttgcaacca
    33961 tgtcatcata atatttttct aaatcgatat cgtctaatgt cacaccatat tcttctttaa
    34021 ctgtatgagg tgcaaacata taaaagtcct taccttcttt agctaaatcg aagaatttag
    34081 atggaacaat taaaccagtt gatattgtag ataaacgtaa atcttcatcc gcatttactt
    34141 ttttagtatc taaaaattct tctacatcat aatggaagat atttaagtac acagcaccag
    34201 caccagggcg ttgaccaagt tgatctgcat agctaaagcc accttcaagt gacttagcaa
    34261 taggtaaaac gcctttcgct acgcctttaa ttcctttaat tgcttcacca cgtgcacgca
    34321 atttagataa gttaattgca acgccgcccc caattttact taattgtttt gcagttgaat
    34381 caataaagtt aattgaattt aagctgtcat ccacttctaa taagaaacat gacactagct
    34441 caccacgacg cgcacggcct gcgtttaaaa atgttggtgt cgctggttga tatctttgtt
    34501 caaccatagc agaaataaat tgtttagctt gtgctttatt accatttgct aggtataaag
    34561 caacaatggc aacgtgttga ttatagtctt ctaagtattg acttttatca tttgttttca
    34621 aagcgtaatc tttgaaaaat ttactagctg acatataact tgcaaaatta aacgggattg
    34681 attttgcata atcagtgatt tcaattagat ccgcttcact ataaatatca aacacattga
    34741 aataaaaatc gttgtctact aaataacgta aacgctcgat ttcagtgtcg aagaagattg
    34801 ttttgtcttt tacttcttct aaataagcta ctaaagcttc ttggtctttt tctaaactaa
    34861 agaatccatc ttctcttcgt ttagtgacct cattatttaa ttcaatatga ttgtactttt
    34921 tctcttccat ggttttcatt aaaattaccc accttgttct taaattcaat aacgtctttg
    34981 ttttttccat gtaactcaaa cttcattaat aaagggacat tatactcttc tgatatcgtg
    35041 cgacccgctt ttgcgaaatt tagtccccaa tttcgattac cgctagctgc cacacctctg
    35101 atgtattgat gattaacttc taaaaaagat tgaacgggtt ctggtacttc tccaaatcca
    35161 atagtgccag taacgataat aaacggttca tgaactggtt ccatacaatt ttctgctgta
    35221 atctcaagcg tattttcaag ttctgttctc ttaataaaac gacggacatt tccagtaaat
    35281 gaaaaatata ttattttcat tggatcacct tctttcttca acccttttaa ataaagtagt
    35341 cattccaatt aaaaaatgct ttaacaacat ggttactatg gttatctcag cattgtatta
    35401 actataatga agctacttat tttctcaaat gtaaaccaat tgcatagatt tatatttgag
    35461 aaaatagtag cttatatggg ttgaatgtat aaaaatcact atatgtagtg tttacatcat
    35521 tggatatctt ccatttgctc agtacatttt ctaaaaat
//
LOCUS       DALAGD010000016        34246 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.016, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000016 DALAGD010000000
VERSION     DALAGD010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 34246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34246
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.016"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            91..411
                     /locus_tag="O2701_000577"
     CDS             91..411
                     /locus_tag="O2701_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HCZ4292487.1"
                     /translation="MPHILNVTVPIPETHVLITKDEYDELIGYSLDPVWNMSDLKKKL
                     KIASDETIKDRLLFHPRFEKELRAQGIVHYPDESFNRWRFNARKMNKFVDEHFNEIYK
                     ERIK"
     gene            408..569
                     /locus_tag="O2701_000578"
     CDS             408..569
                     /locus_tag="O2701_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
                     /protein_id="HCZ4292488.1"
                     /translation="MSDTYKSYLLAVLCFTVLAIVLMPLLYFTTAWSIAGFASIGTFI
                     YYKEYFYEE"
     gene            664..924
                     /locus_tag="O2701_000579"
     CDS             664..924
                     /locus_tag="O2701_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001794769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2482 family protein"
                     /protein_id="HCZ4292489.1"
                     /translation="MTKNYKDMTQDELRGLLAEKNAELFDLASEIDEETEFDVLLFSN
                     VGISNGDFTPSSHCVIGNVVDIANLLKRRAVYRDIADVIKMR"
     gene            964..1224
                     /locus_tag="O2701_000580"
     CDS             964..1224
                     /locus_tag="O2701_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HCZ4292490.1"
                     /translation="MYYKTGDVCRKIINVDGFDFQLRVKKRAYSVEMVVLDHEGNSID
                     GLLVSDENDLYTALDILKQSVYEWIENNTDEQDKLMNLVMKW"
     gene            1233..1496
                     /locus_tag="O2701_000581"
     CDS             1233..1496
                     /locus_tag="O2701_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001638663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292491.1"
                     /translation="MRDTERNILNIFKTLFDEYTLSNQRALLEIERNHHGYLSINFLH
                     YHDSYKTNNKLVQIHEINPDSHERIKNLIIEVLRGHRKIKKGA"
     gene            1505..3448
                     /locus_tag="O2701_000582"
     CDS             1505..3448
                     /locus_tag="O2701_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCZ4292492.1"
                     /translation="MKINKLTISNFAGIKEEKFNFDGKDAKIYGNNATGKTTTATALQ
                     WLLFDKGLDGSTKSFNPVPLNEKNEENYELIPTVFAEFEIDGKITTFKKESHPKYTIN
                     QKTNRKEYSRSRTKKQYINDESIKVKDYKARIDELIDEDVFKLITNPQAFNLLDWKKR
                     RSLLFEIAKPINDEDVIKTNDDFKELNNILGDHEIETKKKILTDKIKQINKDIKDIPI
                     RINQTQQNKQDVPEFDNDRYAIIKQEIEQLENERIDIQNGKEEINLRNQLADKQSELK
                     RIEDNNSASNENKIHALTNELHVENGTVANLKTRLKQNKQQITHEENRRNQLLENHKG
                     LKSDLEKAKNQKFEYLDDNVCSCCGQKLPVEQVDMAREKALQKFNASKSKELETIQTS
                     INHIISEGKKIKLIIEKLEDDNNNLQIKINEAEERSARIQNKINKLKTTHVDVTQTDE
                     YKAVMLEINEINQKRSNIRKTIQDKVSGIDDKISELTQEKSEIEVSRSIEKSNKHLDD
                     VISELRNEEDRLLDEKEKYSHDLYILKEFTTTKVKMLTENINNEFEIAEFKLFNTLVN
                     GELEETCSTTVNGVEYDSGLNNASRINVGLDIINTLSKHFKVTAPIFIDNAESVTELI
                     KTESQQIQLIVNEQDKKLRMETI"
     gene            3450..4370
                     /locus_tag="O2701_000583"
     CDS             3450..4370
                     /locus_tag="O2701_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HCZ4292493.1"
                     /translation="MTENNKLQTIEQQLVQEKNVSDNVLNKVRVLESQGNLELPNDYS
                     PSNAMKQAWLQISQDNKLMSCNDTSKANALLDMVTQGLNPAKNQCYFIPYGNKMQLQR
                     SYHGNVMMLKRDAGAQDVVAQVIYKGDTFKQEMGETGRIKAIKHEQDFFNIDKENIIG
                     AYCTIVFNDGRDNYIEVMTIEQIKQAWMQSSMIKDEKALQNSKTHNNFKEEMAKKTVI
                     NRAAKRYINTSTDSNLFKYAQESEQRQRKEVLDAEVEENANQEQLDFEQPVLEEAQYT
                     ELENDKPIDVSDFEEIKEPATEKESEEEPF"
     gene            4451..5068
                     /locus_tag="O2701_000584"
     CDS             4451..5068
                     /locus_tag="O2701_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCZ4292494.1"
                     /translation="MKFERVQKHFKYKTRHIAGCLITHEHGDHAKYTKQFVDNGVISY
                     MTAGTQQAMNFESHRLCTIKAKQELRIGTWSILPFDIEHDANEPVAFLLQSTLGYKVL
                     YVTDTKYLKYKFNGITHMMLEVNYIYEQMQENIKNGSVHSTLANRIMESHFSLEHAIG
                     MLKANDLTRLEEIHLIHLSSQNSNAKYIKSEIQKVTGAPVYFGGL"
     gene            5069..5539
                     /gene="ssb"
                     /locus_tag="O2701_000585"
     CDS             5069..5539
                     /gene="ssb"
                     /locus_tag="O2701_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCZ4292495.1"
                     /translation="MLNRTILVGRLTRDPELRTTQNGVNVASFTLAVNRTFTNAQGER
                     EADFINIIVFKKQAENVNKYLSKGSLAGVDGRLQTRNYENKEGQRVYVTEVIADSIQF
                     LEPKNSNDTQQDLYQQQVQQTRGQSQYSNNKPVKDNPFANANGPIELNDDDLPF"
     gene            5569..6429
                     /locus_tag="O2701_000586"
     CDS             5569..6429
                     /locus_tag="O2701_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HCZ4292496.1"
                     /translation="MTGWISIDRSIQNHWLFKEKRTFSKFEAWIYLLMEANHSKAKVP
                     IGNQIVTVERGQRLTSILTLSDLFNWSRFKVKTFLDLLESDGMLEVKTTSKYTLITIV
                     NYDFYQSEQGRNQHQNDIKPTSKQHQTNTNNNDNKDNNEKNVNNEKKKTTAFDFFQDN
                     GFGFITSYNLDDLNYYLDSFENDSDEIVTASLKIAKDRNKVTWGYAKSILNTWLNANL
                     KSIEQVRAFEKQQLESKKQNYKPFVKQSKEKTPKWLTDSTRETKTPEVDENLEKDREA
                     FIKRLNSKWE"
     gene            6436..6654
                     /locus_tag="O2701_000587"
     CDS             6436..6654
                     /locus_tag="O2701_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292497.1"
                     /translation="MDAFDKYYLFDHDGNKMFSVTPHFKDGRHLVVGLKHTKFNGRRW
                     YLDDYELKTLIDNEQMELGHQTSLFEYI"
     gene            6663..7067
                     /locus_tag="O2701_000588"
     CDS             6663..7067
                     /locus_tag="O2701_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCZ4292498.1"
                     /translation="MEIEIKFNEVFNAPMGSPRPRFSTKGRYAHTYMPTKYTEHKKYL
                     QNQMPKLNLENALKIELEFYFTPPKSWSKKKKTQAIGQLKVTKPDIDNLMKTVLDACN
                     NYLWKDDNQITEITSSKRYGIEPKIIIRIEEI"
     gene            7080..7448
                     /locus_tag="O2701_000589"
     CDS             7080..7448
                     /locus_tag="O2701_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292499.1"
                     /translation="MARKARIVTINDKPYRFTKSEMELIESHGITAGMVSKRVKDGWE
                     LHEAMDAPEGTRLSEYREKKTIERLEQARLERKLERKRKKEAELRRKKPHLFNVPQKH
                     PRGRYACYLMENDIFVKVKK"
     gene            7452..7694
                     /locus_tag="O2701_000590"
     CDS             7452..7694
                     /locus_tag="O2701_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phi PVL orf 51-like protein"
                     /protein_id="HCZ4292500.1"
                     /translation="MTDNARKEYLNQFFGSKRYLYQDNERVAHIHVVNGTYYFHGHIV
                     PGWQGVKKTFDTAEELETYIKQQGLEYEEQKQLTLF"
     gene            7703..8074
                     /locus_tag="O2701_000591"
     CDS             7703..8074
                     /locus_tag="O2701_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCZ4292501.1"
                     /translation="MKVNYETGFQIGVMEARLKKMRKQRDEYKKQRDELIGDIAKLRE
                     RNEELENMWRTLKNELFGRYEFYRFRLSELQIESRANKEVAIYRRAEINLSVILCRMD
                     KLDGTNEFYEFLGQMEDDTNE"
     gene            8067..8303
                     /locus_tag="O2701_000592"
     CDS             8067..8303
                     /locus_tag="O2701_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HCZ4292502.1"
                     /translation="MNNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEIRE
                     AINAQWVEYPEDWASEVLREVRELEYEEEQENEN"
     gene            8293..8535
                     /locus_tag="O2701_000593"
     CDS             8293..8535
                     /locus_tag="O2701_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292503.1"
                     /translation="MKINESLKKLKEKGYKENEDKAIFNLADGTLEIYIDHDEKTIIT
                     EFHDLKVFVSEDLKDKSMESVMYELAGIDEEDKEND"
     gene            8528..9061
                     /locus_tag="O2701_000594"
     CDS             8528..9061
                     /locus_tag="O2701_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP pyrophosphatase"
                     /protein_id="HCZ4292504.1"
                     /translation="MTNILQVKLLSENARMPERNHKTDAGYDIFSAETVVLEPQEKAV
                     IKTDVAVSIPEGYVGLLTSRSGVSSKTHLVIETGKIDAGYHGNLGINIKNDNETLESE
                     DMSNFGRSPAGIDGKYARLPVTDKILCMNGSYVINKGDKLAQLVIVPIWTPELKQVEE
                     FESVSERGAKGFGSSGV"
     gene            9098..9343
                     /locus_tag="O2701_000595"
     CDS             9098..9343
                     /locus_tag="O2701_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292505.1"
                     /translation="MSDMLEIFLIGFGVYLFYRIAIIFLKSKKTIHTNIYEMLMLATI
                     FMISTFAYKHQKTHILIAFLVMFFMSKLKQVQGSYEE"
     gene            9340..9546
                     /locus_tag="O2701_000596"
     CDS             9340..9546
                     /locus_tag="O2701_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HCZ4292506.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITKAKSNQRFTVVEAESKEEAKEKY
                     EKQVKRDAVIKVGQLFENIRECGK"
     gene            9543..9929
                     /locus_tag="O2701_000597"
     CDS             9543..9929
                     /locus_tag="O2701_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292507.1"
                     /translation="MIKKLKNMDGFDIFIVGILSLFGITALLLVVALPIYTVASYQNK
                     EVHQGTITDKYNKRQDKEDKFYIVLDDKQVIENSDLFFKGKFDSADIQARLKVGDKVK
                     VKTIGYRIHFLNLYPVLYEVKKVDKK"
     gene            9926..10075
                     /locus_tag="O2701_000598"
     CDS             9926..10075
                     /locus_tag="O2701_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCZ4292508.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDYVFR
                     AEVSE"
     gene            10075..10275
                     /locus_tag="O2701_000599"
     CDS             10075..10275
                     /locus_tag="O2701_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HCZ4292509.1"
                     /translation="MWITMTIVFAILLLVCISINSDRAREIQALRYMNDYLLDEVVKT
                     KGYNGLKEYRIELKRMNNDIKK"
     gene            10303..10719
                     /locus_tag="O2701_000600"
     CDS             10303..10719
                     /locus_tag="O2701_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCZ4292510.1"
                     /translation="MIKIEKHDIKKLEEYIQHIDNYRRELKMREYELLESHEPDNAGA
                     GKSNLPGNPIERCAIKKFSDNRYNTLRNIVNGVDRLIDESDEDTLELLRFRYWDCPIG
                     CYEWEDIAHYFGTSKTSILRRRNALIDKLAKYIGYV"
     gene            10951..11250
                     /locus_tag="O2701_000601"
     CDS             10951..11250
                     /locus_tag="O2701_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCZ4292511.1"
                     /translation="MMTKDERIRFYKSKEWQTTRKRVLERDNYECQQCKRDGKLTTYD
                     KSKRKSLDVDHILSLEHHPEFAHDLNNLETLCIKCHNKKEKRFIKKENKWKDEKW"
     gene            11380..11724
                     /locus_tag="O2701_000602"
     CDS             11380..11724
                     /locus_tag="O2701_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292512.1"
                     /translation="MEIIVDENLVLKEKERLQVLYKDIPSNKLKVVDGLIIQAARLRV
                     MLDYMWEDIKEKGDYDLFTQSEKAPPYERERPVAKLFNARDAAYQKIIKQLSDLLPEE
                     KEDTETPSDDYL"
     gene            11721..13382
                     /locus_tag="O2701_000603"
     CDS             11721..13382
                     /locus_tag="O2701_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCZ4292513.1"
                     /translation="MISNKYVDEYINLWKQGKIILNKERIDLFNYLQKHIYSRDDVYF
                     DEQKIEDCIKFIEKWYFPTLPFQRFIIANIFLIDKNTDEAFFTEFAIFMGRGGGKNGL
                     ISAISDFLSTPLHGVKEYHISIVANSEDQAKTSFDEIRTVLMDNKRNKTGKTPKAPYE
                     VSKAKIINRATKSVIRYNTSNTKTKDGGREGCVIFDEIHYFFGPEMVNVKRGGLGKKK
                     NRRTFYISTDGFVREGYIDAMKHKIASVLSGKVKNSRLFAFYCKLDDPKEVDDRQTWE
                     KANPMLHKPLSEYAKTLLSTIEEEYNDLPFNRSNKPEFMTKRMNLPEVDLEKVIAPWK
                     EILATNREIPNLDNQMCIGGLDFANIRDFASVGLLFRKNDDYIWLGHSFVRQGFLDDV
                     KLEPPIKEWEKMGLLTIVDDDVIEIEYIVDWFLKAREKYGLEKVIADNYRTDIVRRAF
                     EDAGIKLEVLRNPKAIHGLLAPRIDTMFAKHNVIYGDNPLMRWFTNNVAVKVKPDGNK
                     EYIKKDENRRKTDGFMAFVHALYRADDIVDKDMSKALDALMSIDF"
     gene            13398..14585
                     /locus_tag="O2701_000604"
     CDS             13398..14585
                     /locus_tag="O2701_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCZ4292514.1"
                     /translation="MSILEKIFKTRKDITYMLDLDMIEDLSQQAYVKRLAIDSCIEFV
                     ARAVAQSHFKVLEGNRIQKNDVYYKLNIKPNTDLSSDSFWQQVIYKLIYDNEVLIVVS
                     DSKELLIADSFYREEYALYDDIFKDVTVKDYTYQRTFTMQEVIYLKYNNNKVTHFVES
                     LFEDYGKIFGRMIGAQLKNYQIRGILKSASSAYDEKNIEKLQAFTNKLFNTFNKNQLA
                     IAPLIEGFDYEELSNGGKNSNMPFSELSELMRDAIKNVALMIGIPPGLIYGETADLEK
                     NTLVFEKFCLTPLLKKIQNELNAKLITQSMYLKDTRIEIVGVNKKDPLQYAEAIDKLV
                     SSGSFTRNEVRIMLGEEPSDNPELDEYLITKNYEKANSGENDEKEKDENTLKGGDEDE
                     SGD"
     gene            14569..15306
                     /locus_tag="O2701_000605"
     CDS             14569..15306
                     /locus_tag="O2701_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCZ4292515.1"
                     /translation="MKAEIKGVIVSNEDKWVYEMLGMDSTCPKDVLTQLEFSDEDVDI
                     IINSNGGNLVAGSEIYTHLRAHKGKVNVRITAIAASAASLIAMAGDHIEMSPVARMMI
                     HNPSSIAQGEAKDLNHAAETLEHVGQIMAEAYAVRAGKNKQELVEMMARETWLNADEA
                     IEQGFADSKMFENDNMQIVASNTQVLSKDVLNRVTALVSKTPEVNIDIDAIANKVIEK
                     INMKEKESEIDVADSKVSANGFSRFLF"
     gene            15330..16475
                     /locus_tag="O2701_000606"
     CDS             15330..16475
                     /locus_tag="O2701_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCZ4292516.1"
                     /translation="MTINLSETFANAKNEFINAVNNGEPQERQNELYGDMINQLFEET
                     KLQAKAEAERVSSLPKSAQSLSANQRSFFMDINKNVNYKEEKLLPEETIDRIFEDLTT
                     NHPLLADLGIKNAGLRLKFLKSETSGVAVWGKIYGEIKGQLDAAFSEETAIQNKLTAF
                     VVLPKDLNDFGPAWIERFVRVQIEEAFAVALETAFLKGTGKDQPIGLNRQVQKGVSVT
                     EGAYPEKEEQGTLTFANPRATVNELTQVFKYHSTNEKGKSVAVKGNVTMVVNPSDAFE
                     VQAQYTHLNANGVYVTALPFNLNVIESTVQEAGKVLTYVKGLYDGYLAGGINVQKFKE
                     TLALDDMDLYTAKQFAYGKAKDNKVAAVWKLDLKGHKPALEGTEETL"
     gene            16495..16779
                     /locus_tag="O2701_000607"
     CDS             16495..16779
                     /locus_tag="O2701_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292517.1"
                     /translation="MVKFKVVRAFKDIEHNQHKYKVGELYPAEGYNNPRVELLTNQIK
                     NKYDKVYIVPLDKLTKQELLELCESLQKKASSSMVKSEIVDLLNGEDNDD"
     gene            16769..17053
                     /locus_tag="O2701_000608"
     CDS             16769..17053
                     /locus_tag="O2701_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HCZ4292518.1"
                     /translation="MTIDDLLVKFKSLEKIDHNSEDEYLKQLLKMSYERIKNQCGVFE
                     LENLIGQELILIRARYAYQDLLEHFNDNYRPEIIDFSLSLMEVSEDEESV"
     gene            17037..17399
                     /locus_tag="O2701_000609"
     CDS             17037..17399
                     /locus_tag="O2701_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HCZ4292519.1"
                     /translation="MKKVFKKPRITTKRLNTRVHFYKYTENNGPEAGEKEEKLLYSCW
                     ASIDGVWLRELEQAISNGTQNDIKLYIRDPQGDYLPSEEHYLEIESRYFKNRLNIKQV
                     SPDLDNKDFIMIRGGYSS"
     gene            17396..17800
                     /locus_tag="O2701_000610"
     CDS             17396..17800
                     /locus_tag="O2701_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HCZ4292520.1"
                     /translation="MSVKVIGDKALERELENRFGTQAMLKVQDRALIAGAKVIVEEVK
                     KQLKPSKDTGALINEVSFSKPEWINGKRTITVHWRGSKDRYKIVHLIENGHVQKGTGK
                     FIKPKAMGGVNRAIRQGQNKYFETLKRELKKL"
     gene            17797..18201
                     /locus_tag="O2701_000611"
     CDS             17797..18201
                     /locus_tag="O2701_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292521.1"
                     /translation="MIDILYKVHEVISQDRIIREHVNINNIKFNKYPNVKDTDVPFIV
                     IDDIDDPIPTTYTDGDERAYSYIVQIDVFVKFNEKYNARIIRNKISNRIQKLLWSELK
                     MGNVSNGKPEYIEEFKTYRSTRVYEGIFYEEE"
     gene            18205..18849
                     /locus_tag="O2701_000612"
     CDS             18205..18849
                     /locus_tag="O2701_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCZ4292522.1"
                     /translation="MVVKQASAPKAYFNITGLGFAKLTKEGAKLEYSDITKTRGLQKI
                     GVETGGDLKTAYADGGPIESGNTDGEGKISLQMHAFPKEIRKIVFNEEYDEYGVYKET
                     QGKQNNYVAIWFRQERRDGTFRTVLLPKVMFTNPKIDGETAEKDWDFSSEEVEGEALF
                     PLIDNKKSVRKYIFDSANMTNHSGNGEKGEEAFLKKILGEEYTGNVTEDNGETL"
     gene            18876..19115
                     /locus_tag="O2701_000613"
     CDS             18876..19115
                     /locus_tag="O2701_000613"
                     /inference="COORDINATES: protein motif:HMM:NF014427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCZ4292523.1"
                     /translation="MKSVNIPDSFKTLKVGNTYDLNVVVEPSNQSKLLKYTTDQTNIV
                     SINSDGQVTAEAQGIATVKAYVGNMSDTITINVEA"
     gene            19165..19515
                     /locus_tag="O2701_000614"
     CDS             19165..19515
                     /locus_tag="O2701_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292524.1"
                     /translation="MAKLKRNIIQLVEDPKANEIKLQTYLTPHFISFEIVYEAMDLID
                     DIEDENSTMKPREIADRLMDMVVKIYDNQFAVKDLKERMHAPDGMNALREQVVFITQG
                     QQTEETRNFIQNMK"
     gene            19566..19703
                     /locus_tag="O2701_000615"
     CDS             19566..19703
                     /locus_tag="O2701_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292525.1"
                     /translation="MMDLIENGKDANEVLKMPFHYVLSIYQNKNNDISEEKAEALIDA
                     F"
     gene            19760..24289
                     /locus_tag="O2701_000616"
     CDS             19760..24289
                     /locus_tag="O2701_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001799555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCZ4292526.1"
                     /translation="MGERIKGLSIGLDLDAANLNRSFAEIKRNFKTLNSDLKLTGNNF
                     KYTEKSTDSYKQRIKELDGTITGYKKNVDDLAKQYDKVSQEQGENSAGAQKLRQEYNK
                     QANELNYLERELQKTSAEFEEFKKAQVEAQRMAESGWGKTSKVFESMGPKLTKMGDGL
                     KSIGKGMMIGVTAPVLGIAAASGKAFAEVDKGLDTVTQATGATGSELKKLQNSFKDVY
                     GNFPADAETVGGVLGEVNTRLGFTGKELENATESFLKFSHITGSDGVQAVQLITRAMG
                     DAGIEASEYQSVLDMVAKAAQASGISVDTLADSITKYGAPMRAMGFEMKESIALFSQW
                     EKSGVNTEIAFSGLKKAISNWGKAGKNPREEFKKTLAEIEKTPDIASATILAIEAFGA
                     KAGPDLADAIKGGRFSYQEFLKTIEDSQGTVNQTFKDSESGSERFKVAMNKLKLVGAD
                     VWASIESAFAPVMEELIKKLSVAVDWFSNLSDGSKRSIVIFGGIAAAIGPVVFGLGAF
                     ISTIGNAVTVLAPLLAGIAKADGLISFLSTKVPILGTVFTALTGPIGIVLGVLAGLAV
                     AFTIAYKKSETFRNFVNDAIESVKQTFSNFIQFIQPFIDSVKNVFKQAVSAIVDFAKD
                     IWSQINGFFNENGISIVQALQNICNFIKVIFEFILNFVIKPIMFAIWQVMQFIWPAVK
                     ALIVSTWENIKGVIQGTLNIILGLIKFFSSLFTGDWRGVWDAIIMILKGVVQLIWNLI
                     QLWFVGKILGVVRYFGGLLKGLITGIWDVIKSIFSKSLSAIWNATKSIFGFLFNSVKS
                     IFTNMKNWLSNTWSSIRTNTIGKAQSLFSGVKSKFTSLWNATKDIFSNLRNWMANIWN
                     SIKDNTVGIASRLWSKVRGIFTNMRDGLQSIISKIKSHIGGMVDAIKKGLNKLIEGLN
                     WVGGKLGMDKIPKLHTGTEHTHTTTRLVKNGKIARDTFATVGDKGRGNGPNGFRNEII
                     EFPNGKRVITPNTDTTAYLPKGSKVYNGTQTYSMLNGTLPRFSLGTMWKDIKSGASSA
                     FNWTKDQIGKGTKWLGDKVGDVLDFMENPGKLLNYILEAFGIDFNSLTKGMGIAGDIT
                     KAAWSKIKKSATDWIKENLEAMGGGDLVGGILDPDKINYHYGRTAAYTAATGRPFHEG
                     VDFPFVYQEVRTPMGGRLTRMPFMSGGYGNYVKITSGVIDMLFAHLKNFSKSPPSGTM
                     VKPGDVVGLTGNTGFSTGPHLHFEMRRNGRHFDPEPYLRNAKKKGRLSIGGGGATSGS
                     GATYASRVIRQAQSILGGRYKGKWIHDQMMRVAKRESNYQSNAVNNWDINAQRGDPSR
                     GLFQIIGSTFRANAKRGYTNFNNPVHQGISAMQYIVRRYGWDGFKRAGDYAYATGGKV
                     FDGWYNLGEDGHPEWIIPTDPARRNDAMKMLHYAAAEVRGRKASKNKRPSQLSSVNGF
                     DDPSLLLKMIEQQQQQIALLLKIAQSNDVIADKDYQPIIDEYAFDKKVNASIEKRERQ
                     ESTKVKFRKGGIAIQ"
     gene            24286..25770
                     /locus_tag="O2701_000617"
     CDS             24286..25770
                     /locus_tag="O2701_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HCZ4292527.1"
                     /translation="MIDTIKVNNKTIPWLYVERGFEIPSFNYVLKTENVDGRSGSIYK
                     GRRLESYSFDIPLVVRNDYLSHNGFKTHDDVLNELVKFFNYEEQVKLQFKSKDWYWNA
                     YFEGPIKLHKEFAIPVKFTIKVVLTDPYKYSVTGYKNTAISDQVSVVNSGTADTPLIV
                     EARAIKPSSYFMITKNDEDYFMVGDDEVTKEVKDYMPPVYHSEFRDFKGWTKMITEDI
                     PSNDLGGKVGGDFVISNLGEGYKATNFSDAKGWVGAGTKRGLPKAMTDFQITYKCIVE
                     QKGKGAGRTAQHIYDSDGKLLASIGYENKYHDRKIGHIVVTLYNQKGDPKKIYDYQNK
                     PIMYNLDRIVVYMRLRRVGNKFSIKTWKFDHIKDPDRRKPIDMDEKEWIDGGKFYQRP
                     ASIIAIYSAKYNGYKWMEMNGLGSFNTEILPKPKGARDVIIQKGDLVKIDMQAKSVVI
                     NEEPMLSEKSFGSNYFNVDSGYSELIIQPENVFDTTVKWQDRYL"
     gene            25786..29571
                     /locus_tag="O2701_000618"
     CDS             25786..29571
                     /locus_tag="O2701_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292528.1"
                     /translation="MIHVLDFNDKIIDFLSTDDPSLVRAIHKRNVNDNSEMLELLISS
                     ERAEKFRERHRVIIRDSNKQWREFIINWVQDTMDGYTEIECIASYLADITTAKPYAPG
                     KFEKKTTSEALKDVLSDTGWEVSEQTEYDGLRTTSWTSYQTRYEVLKQLCTTYKMVLD
                     FYIELSSNTVKGRYVVLKKKNSLFKGKEIEYGKDLVGLTRKIDMSEIKTALIAVGPEN
                     DKGKRLELVVTDDEAQSQFNLPMRYIWGIYEPQSDDQNMNETRLSSLAKTELNKRKSA
                     VMSYEITSTDLEVTYPHEIISIGDTVRVKHRDFNPPLYVEAEVIAEEYNIISENSTYT
                     FGQPKEFKESELREEFNKRLNLIHQKLNDNISNINTIVKDVVDGELEYFERKIHKSDT
                     PPENPVNDTLWYDTSNPDVAVLRRYWNGRWIEATPNDVEKLGGITREKALFSELNNIF
                     INLSIQHASLLSEATELLNSEYLVDNDLKADLQASLDAVIDVYNQIKNNLESMTPETA
                     TIGRLVDTKTLFLEYRKKLQDVYTDVEDVKIAISDRFKLLQSQYTDEKYKEALEIIAT
                     KFGLTVNEDLQLVGEPNVVKSAIEAARESTKEQLRDYVKTSDYKTDKDGIVERLDTAE
                     AERTTLKGEIKDKVTLNEYRNGLEEQKQYTDDQLSDLSNNPEIKASIEQANQEAQEAL
                     KSYIDAQDNLKEKESQAYADGKISEEEQRAIQDAQAKLEEAKQNAELKARNAEKKANA
                     YTDNKVKESTDAQRRTLTRYGSQIIQNGKEIKLRTTKEEFNATNRTLSNILNEIVQNV
                     TDGTTIRYDDNGVAQALNVGPRGIRLNADKIDINGNREINLLIQNMRDKVDKTDIVNS
                     LNLSREGLDINVNRIGIKGGNNNRYVQIQNDSIELGGIVQRTWKGKRSTDDIFTRLKD
                     GHLRFRNNTAGGSLYMSHFGISTYIDGEGEDGGSSGTIQWWDKTYSDSGMNGITINSY
                     GGVVALTSDNNRVVLESYASSNIKSKQAPVYLYPNTDKVPGLNRFAFTLSNADNAYSS
                     DGYIMFGSDENYDYGAGIRFSKERNKGLVQIVNGRYATGGDTTIEAGYGKFNMLKRRD
                     GNRYIHIQSTDLLSVGSDDAGDRIASNSIYRRTYSAAANLHITSAGTIGRSTSARKYK
                     LSIENQYNDRDEQLEHSKAILNLPIRTWFDKAESEILARELREDRKLSEDTYKLDRYV
                     GLIAEEVENLGLKEFVTYDDKGEIEGIAYDRLWIHLIPVIKEQQLRIKKLEESKNAG"
     gene            29561..29713
                     /locus_tag="O2701_000619"
     CDS             29561..29713
                     /locus_tag="O2701_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292529.1"
                     /translation="MQDNKQGLQANPEYTIHYLSQEIMRLTQENAMLKAYIQENKENQ
                     QCAEEE"
     gene            29760..30047
                     /locus_tag="O2701_000620"
     CDS             29760..30047
                     /locus_tag="O2701_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292530.1"
                     /translation="MAKEIINNTERFILVQIDKEGTERVVYQDFTGSFTTSEMVNHAQ
                     DFKSEENAKKIAETLNLLYQLTNKKQRVKVVKEVVERSDLSPEVTVNTETV"
     gene            30105..30401
                     /locus_tag="O2701_000621"
     CDS             30105..30401
                     /locus_tag="O2701_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 domain-containing protein"
                     /protein_id="HCZ4292531.1"
                     /translation="MGWFKKHEHEWRIRRLEENDKTMLSTLNEIKLGQKTQEQVNIKL
                     DKTLDAIQKEREIDEKNKKENDKNIRDMKMWVLGLVGTIFGSLIIALLRMLMGI"
     gene            30551..30727
                     /locus_tag="O2701_000622"
     CDS             30551..30727
                     /locus_tag="O2701_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HCZ4292532.1"
                     /translation="MDRWWLSEYKEVVPMVALLKSLERRRLMITISTMLQFGLFLIAL
                     IGLVIKLIELSNKK"
     gene            complement(30780..30887)
                     /locus_tag="O2701_000623"
     CDS             complement(30780..30887)
                     /locus_tag="O2701_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292533.1"
                     /translation="MIFILWCVNLYIEKGQHAQTCYPNEPVKKTVAILD"
     gene            30939..31193
                     /locus_tag="O2701_000624"
     CDS             30939..31193
                     /locus_tag="O2701_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCZ4292534.1"
                     /translation="MINWKIRMKQKSFWVAILSAIFLFAQNIAKAIGYDIQVYTEQLT
                     DGLNAILGFLVLTGVIQDPTTKGIGDSHQALEYEEPRRKY"
     gene            31205..31960
                     /locus_tag="O2701_000625"
     CDS             31205..31960
                     /locus_tag="O2701_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCZ4292535.1"
                     /translation="MKTYSEARARLRWYQGRYIDFDGWYGYQCADLAVDYIYWLLEIR
                     MWGNAKDAINNDFKNMATVYENTPSFVPQIGDVAVFTKGIYKQYGHIGLVFNGGNTNQ
                     FLILEQNYDGNANTPAKLRWDNYYGCTHFIRPKYKSEGLMNKITNKVKPPAQKAVGKS
                     ASKITVGSKAPYNLKWSKGAYFNAKIDGLGATSATRYGDNRTNYRFDVGQAVYAPGTL
                     IYVFEIIDGWCRIYWNNHNEWIWHERLIVKEVF"
     gene            32151..32642
                     /gene="sak"
                     /locus_tag="O2701_000626"
     CDS             32151..32642
                     /gene="sak"
                     /locus_tag="O2701_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylokinase"
                     /protein_id="HCZ4292536.1"
                     /translation="MLKRGLLFLTVLLLLFSFSSITNEVSASSSFDKGKYKKGDDASY
                     FEPTGPYLMVNVTGVDGKGNELLSPHYVEFPIKPGTTLTKEKIEYYVEWALDATAYKE
                     FRVVELDPSAKIEVTYYDKNKKKEETKSFPITEKGFVVPDLSEHIKNPGFNLITKVII
                     EKK"
     gene            33292..33627
                     /locus_tag="O2701_000627"
                     /pseudo
     CDS             33292..33627
                     /locus_tag="O2701_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
     gene            complement(33722..34171)
                     /locus_tag="O2701_000628"
     CDS             complement(33722..34171)
                     /locus_tag="O2701_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-inhibiting protein CHIPS"
                     /protein_id="HCZ4292537.1"
                     /translation="MKKKLATTVLALSFLTAGISTHHHSAKAFTFEPFPTNEEIESNK
                     KMLEKEKAYKESFKNSGLPTTLGKLDERLRNYLKKGTKNSAQFEKMVILTENKGYYTV
                     YLNTPLAEDRKNVELLGKMYKTYFFKKGESKSSYVINGPGKTNEYAY"
BASE COUNT        13000 a         4777 c         6851 g         9618 t
ORIGIN      
        1 tctaaatatt gagtgagcaa tctcttatgc atacttatca cctcctttca ctaggagata
       61 acaacattat acacgaaagg aaagatagaa atgccacaca ttttaaacgt aacagttcca
      121 atacctgaaa cacatgtact tatcacaaaa gatgaatatg atgagctaat tggttattca
      181 ttagaccctg tatggaacat gagtgactta aagaagaaat taaaaattgc atctgatgag
      241 actatcaagg acagattact atttcatcct agatttgaaa aagaactaag agcgcaagga
      301 attgtgcatt acccagatga gagttttaat cgctggagat ttaacgcaag aaagatgaat
      361 aaattcgtcg atgagcattt caatgaaata tataaggaga gaataaaatg agcgacacat
      421 ataaaagcta cctattagca gtgctatgtt tcacagtctt agcgattgtg cttatgccat
      481 tgctgtactt cactacagcg tggtcaattg caggattcgc aagcattgga acattcatat
      541 actacaaaga atacttttat gaagaataaa aaaactgcta cttgcgtcaa caagtaacag
      601 tgacaaacga ttaacaaaat taattcattt tcaatataaa acgaaacaag gaggaagtca
      661 accatgacta aaaattataa agatatgact caagacgaat taaggggttt attggctgaa
      721 aagaatgcag aattgtttga tttagcgagc gaaatcgatg aagaaactga atttgacgtt
      781 ttactttttt caaatgtagg gattagcaac ggagatttta caccgtcgtc acattgtgtg
      841 attgggaatg ttgttgatat tgctaattta ttgaaacgta gagctgttta ccgagatatc
      901 gctgatgtta tcaaaatgcg ttaaaaattc tagctataga taatgacaag gaggacgaga
      961 acaatgtatt acaaaacggg tgacgtatgt cgaaaaataa ttaatgtaga tggctttgat
     1021 tttcaattaa gagttaagaa acgagcgtat agcgtcgaaa tggttgttct agaccatgaa
     1081 ggaaattcaa ttgacgggtt actagtttct gatgagaacg atctatacac agctttagat
     1141 attttgaaac aaagtgttta tgaatggatt gaaaataaca cagatgaaca ggacaaacta
     1201 atgaacttag tcatgaaatg gtaggtataa gcatgagaga tacagaaaga aatatattga
     1261 atatttttaa gacgttattc gacgaatata ctttgtcaaa ccaacgagca ttattggaaa
     1321 ttgaacgtaa tcatcacgga tacttatcga ttaatttctt gcactatcac gacagttaca
     1381 aaacaaacaa taagcttgtg cagatacatg aaatcaatcc agacagccat gaacgaataa
     1441 aaaatttaat tatcgaggta ctaagaggtc atcggaagat taaaaaagga gcatgaggaa
     1501 ggatatgaaa ataaataaat taacaatatc gaactttgcc ggaatcaaag aagaaaaatt
     1561 taattttgac ggtaaagatg caaaaatata cggcaataat gcgactggta agactacaac
     1621 agcaaccgca ttacaatggt tgcttttcga taaaggtttg gacggttcaa ccaaatcatt
     1681 taaccctgta cctttaaacg aaaaaaacga agaaaattat gagttaattc cgactgtttt
     1741 cgcagaattt gaaatcgacg gaaaaattac gacttttaaa aaagagtcac atcctaaata
     1801 cacaataaat caaaagacga atcgcaagga atactcacga agtcgaacga agaaacaata
     1861 tatcaatgat gaatcaataa aagtaaagga ttataaagct cgtattgatg aacttattga
     1921 tgaagatgta ttcaagttaa ttacgaaccc tcaagcattt aacttactag attggaagaa
     1981 gcgaagaagt ttgttgtttg aaattgctaa accaatcaat gatgaggatg tcattaaaac
     2041 aaatgatgat tttaaagaac taaataatat tcttggagat catgaaattg aaacaaagaa
     2101 aaagattctt acggacaaga taaaacagat taacaaagat atcaaagata ttccgatacg
     2161 tattaaccaa acacaacaaa ataagcagga tgtaccagaa ttcgataatg atagatacgc
     2221 aattatcaaa caagaaattg agcaacttga aaatgagcgt atagatattc aaaacggtaa
     2281 ggaagaaatt aatttacgta atcaattagc tgataaacaa tcagaattga aacgcataga
     2341 agacaataac agcgcaagta atgagaacaa aatccatgct ttaacaaatg aattacacgt
     2401 tgaaaatgga acggtagcaa accttaaaac gagattaaag caaaacaaac aacaaatcac
     2461 gcatgaagaa aatagacgta atcaattatt agaaaatcat aaaggattaa aaagtgattt
     2521 agaaaaagct aaaaatcaaa aatttgaata tcttgatgac aatgtatgta gttgttgtgg
     2581 tcaaaagtta ccagttgaac aagtggatat ggcaagagaa aaagcattgc agaaattcaa
     2641 tgctagcaaa tcgaaagaat tagaaacaat acaaacatct atcaatcaca ttatttcaga
     2701 aggcaagaaa ataaagctaa tcatcgagaa gttagaggat gacaataata atcttcaaat
     2761 taaaatcaac gaagcagaag agcgttcagc aagaatacaa aacaaaatta ataagttgaa
     2821 aacaactcac gttgacgtta cgcaaactga cgaatacaaa gcagtaatgt tagagataaa
     2881 tgagattaat caaaaacgct ctaacatcag gaaaactatt caagataaag tttcaggaat
     2941 agatgacaaa ataagcgaac ttactcaaga aaaatcagaa attgaagtgt caagatcaat
     3001 cgaaaaatca aataaacatc tagatgatgt tatttctgaa ttaagaaatg aagaagacag
     3061 attattggat gaaaaagaaa agtattcaca tgacctttat atcttaaaag aatttacaac
     3121 aacaaaagtc aaaatgctta ctgaaaacat caataatgaa tttgaaattg ctgaatttaa
     3181 gctattcaat accttagtta acggcgaatt agaagaaaca tgttccacaa cggttaacgg
     3241 cgtcgaatac gacagcggtt taaataacgc ctcaagaatt aatgttggct tagatatcat
     3301 caatacactg tcaaaacatt ttaaagttac agcgccaata tttattgata atgctgaatc
     3361 agtaacagag cttatcaaaa cagaatcaca acaaattcaa ttgatagtaa atgaacaaga
     3421 taaaaaatta agaatggaga ctatataaaa tgactgaaaa taataaatta caaactattg
     3481 aacaacaatt agtacaagaa aagaacgtat ctgacaacgt attaaacaaa gtgagagttt
     3541 tagagtcaca aggcaatttg gaattgccaa atgattattc accaagtaat gccatgaaac
     3601 aagcatggtt acaaatcagc caagataaca aattaatgag ttgtaacgat acaagcaaag
     3661 caaatgcctt attagacatg gtaacgcaag gtttaaatcc agctaaaaat caatgctact
     3721 ttattcctta cggcaacaaa atgcagttac aacgtagcta tcacggtaat gtaatgatgt
     3781 taaaacgtga tgcaggtgct caagatgttg ttgctcaagt gatttataaa ggcgatacat
     3841 tcaagcaaga aatgggagaa acaggacgta tcaaagcgat taaacacgaa caagacttct
     3901 ttaacatcga caaagaaaac attatcggtg cgtactgcac aatcgtattt aatgatggac
     3961 gagataacta tattgaagtc atgactattg aacaaattaa acaagcatgg atgcagtcat
     4021 caatgattaa agatgaaaaa gcattacaaa attctaaaac acataataat ttcaaagaag
     4081 aaatggctaa aaaaacagtt atcaatagag ctgctaaacg ttatatcaac acatcaacag
     4141 atagcaatct tttcaaatac gcacaagaat ccgaacaacg tcaacgcaaa gaagtgttgg
     4201 acgcagaagt tgaagaaaat gcaaatcaag aacaattgga ctttgaacaa ccagttcttg
     4261 aagaagcaca atacacagaa ttagaaaatg ataagcctat tgatgtatct gactttgaag
     4321 aaataaaaga acctgcaaca gaaaaagaaa gcgaagaaga gccattttaa ttgaaacaat
     4381 agcaactggt tcaagtggta actgctacgt cttaaatgat ggacgtacta cgttactact
     4441 tgaggcaggt ataaaatttg aacgtgttca aaagcatttc aaatataaaa caagacatat
     4501 agcagggtgt cttatcacac acgaacatgg tgatcatgca aagtatacaa agcagtttgt
     4561 cgacaatggt gtaatcagct atatgactgc tggaacacaa caagctatga attttgaaag
     4621 tcatcgctta tgcacgatta aggcaaagca agagctgcga ataggtacat ggtcaattct
     4681 accgtttgac atcgaacatg atgctaacga gcctgtggct ttcttattac aaagcacact
     4741 aggttataag gttctgtatg ttactgatac aaagtatttg aaatacaaat ttaatggcat
     4801 tacgcacatg atgctagaag ttaattatat ctatgaacaa atgcaagaaa acataaaaaa
     4861 cggcagtgtg cacagcacat tagcaaacag aattatggag tctcatttta gcttagaaca
     4921 tgctatcgga atgttaaaag caaatgattt aactagactc gaagaaatac atttaattca
     4981 tttaagtagt caaaattcaa atgcaaaata cattaaaagt gaaatacaaa aagtgacggg
     5041 cgcgcccgtt tattttggag gtttataaat gctaaacaga acaatattag ttggtcgttt
     5101 aactagagac ccagaattaa gaaccactca aaatggtgta aatgtagcat cattcacatt
     5161 agcagttaac cgtacattta caaatgcaca aggcgagcgc gaggcagact ttattaatat
     5221 catcgtattt aaaaaacaag cagagaacgt taataaatac ctatctaaag gatcgttggc
     5281 gggcgtagat ggtaggttac aaacgcggaa ctatgaaaat aaggaaggtc aacgtgtata
     5341 cgttacggaa gttattgctg atagtattca atttttagaa ccgaaaaact caaatgacac
     5401 tcaacaagat ttatatcaac aacaagtaca acaaacacgt ggacaatcgc aatattcaaa
     5461 taacaaacca gtaaaagata atccgtttgc gaatgcaaat ggtccgattg aactaaatga
     5521 tgatgattta ccattctaat ttaaccggtt tgaaagtgag gtgtgtatat gactggttgg
     5581 ataagtattg atcgctcaat tcaaaatcat tggctattta aagaaaagag aacattttca
     5641 aagtttgaag catggatata tttactcatg gaagcgaatc attcaaaggc aaaagtgcct
     5701 ataggaaacc aaattgtaac cgttgaaaga ggacaaagat taacatctat tttgaccttg
     5761 tctgaccttt ttaactggtc acgatttaaa gtgaaaacct tccttgactt actcgagagt
     5821 gatggaatgt tagaagtcaa aacaacatca aaatataccc ttataaccat tgtcaattat
     5881 gacttttatc aaagtgagca gggcaggaac caacatcaaa acgacatcaa accaacatca
     5941 aaacaacatc aaaccaacac aaacaataat gataataaag ataataatga aaagaatgtg
     6001 aataatgaga agaagaagac aaccgccttc gacttcttcc aagataacgg attcggtttc
     6061 ataacttctt acaatttaga cgatttaaat tattatcttg attcatttga aaatgattca
     6121 gatgaaatag ttaccgcatc acttaaaatc gctaaagaca gaaacaaagt tacttgggga
     6181 tatgctaaaa gcattttgaa tacatggctt aatgcaaact tgaaatctat tgaacaagta
     6241 cgtgcatttg aaaagcaaca acttgaaagc aaaaaacaaa attataaacc tttcgttaaa
     6301 caatcaaaag aaaaaacacc caaatggctc acagacagca cgagagaaac gaaaacgccg
     6361 gaagtagatg aaaaccttga gaaagacaga gaagctttta ttaagcgtct aaatagcaaa
     6421 tgggagtgat tgaaaatgga tgcatttgat aaatactatc tatttgatca tgacggcaac
     6481 aaaatgtttt cagttacacc acattttaaa gatggacggc atttagttgt tggattaaaa
     6541 cacacaaaat tcaatggtcg acgttggtac ttagatgatt atgaattaaa aacacttatt
     6601 gataatgaac aaatggagtt aggacaccaa acaagcttat ttgaatatat atgagggatt
     6661 acatggagat agaaattaaa tttaatgaag tgtttaatgc gccgatgggc tcgcctcgac
     6721 cgcgttttag cacaaaaggt agatatgcac acacatatat gcctacaaaa tatacagaac
     6781 ataaaaaata tttacaaaat caaatgccaa agctaaatct agaaaatgca ttaaaaattg
     6841 aattagagtt ttactttact ccacctaaat catggtcgaa gaaaaagaaa actcaagcga
     6901 ttggacaatt aaaagtaact aaaccagata tcgataattt gatgaagaca gttctagacg
     6961 cttgtaacaa ttatttgtgg aaagacgata accaaattac agaaataact agctcaaagc
     7021 gttatggaat tgagcccaaa ataatcatac gaatagaaga aatataagag gtggataaaa
     7081 tggcgagaaa agcaaggatt gtaacaataa atgataaacc atataggttt accaaatctg
     7141 aaatggaatt aatagaaagt cacggtataa ccgctggaat ggtttctaag agagtaaaag
     7201 acggttggga actacatgaa gcaatggacg caccagaagg tacgcgttta agcgagtaca
     7261 gagaaaagaa aacaatagaa agactggaac aagctagact cgaacgcaaa ttggaaagaa
     7321 agcgaaagaa agaggctgag ctaagaagaa agaagccaca tttgtttaat gtaccacaga
     7381 aacatccaag aggacgttat gcgtgctacc tgatggaaaa cgacatattc gtgaaagtta
     7441 agaagtagat catgacagat aacgcacgca aagaatacct aaatcaattc tttggatcta
     7501 agagatatct gtatcaggat aacgaacgag tggcacatat tcatgtagta aacggcactt
     7561 attactttca tgggcatatc gtaccaggtt ggcaaggcgt gaaaaagaca tttgatacag
     7621 ctgaagagct tgaaacatat ataaagcaac agggtttgga atatgaggaa cagaagcaac
     7681 taactttatt ttagaggagg ttatgaaagt gaactatgaa acaggattcc aaataggcgt
     7741 aatggaagct aggttgaaga agatgagaaa acaacgtgat gagtacaaga agcaacgtga
     7801 cgagcttatt ggggatatag ctaagttaag agagcgtaac gaagagctgg agaacatgtg
     7861 gcgcacgctt aaaaatgaat tgtttggaag atacgaattt taccgtttta gacttagcga
     7921 actacagatt gagagcagag cgaacaagga agtagctata tatagaagag ctgaaatcaa
     7981 cttaagtgtt atattgtgcc gaatggacaa actagacgga acaaatgagt tctacgaatt
     8041 tttagggcaa atggaggatg acacaaatga ataaccgcga acaaatcgaa caatcagtta
     8101 tcagtgctag cgcgtataac ggcaatgaca cagagggatt gctaaaagag attgaagacg
     8161 tgtataagaa agcgcaagcg tttgatgaaa ttcgcgaagc tattaatgcg caatgggttg
     8221 agtatccaga agactgggcg tcagaggttt tgagagaagt aagagagctt gaatatgagg
     8281 aggaacagga aaatgaaaat taatgaatct ttgaagaaat taaaagaaaa aggatacaaa
     8341 gaaaatgaag ataaagctat ttttaattta gctgatggta cgctagaaat ttacatcgac
     8401 catgacgaaa aaacaataat tacagaattc catgatttaa aggtattcgt gtcagaagat
     8461 ttaaaagata aaagtatgga gagcgttatg tatgaattgg ctggaattga tgaggaggac
     8521 aaggaaaatg actaacatcc tacaagtaaa gctattatca gaaaacgcta gaatgccaga
     8581 acgaaatcat aagacggatg caggttatga catattctca gctgaaactg tcgtacttga
     8641 gccacaagaa aaggcagtga tcaaaacaga tgtagctgta agcataccag agggctatgt
     8701 cggactatta actagccgta gtggtgtaag tagtaaaacg catttagtga ttgaaacagg
     8761 caagatagac gcgggatatc acggcaattt agggattaat atcaagaatg ataatgaaac
     8821 gttagagagt gaggatatga gtaactttgg tcggagtcct gctgggatag atggaaagta
     8881 tgcccgacta cctgtaacag ataaaatttt atgtatgaat ggtagttatg tcataaacaa
     8941 aggcgacaaa ctagctcaat tggttattgt gcctatatgg acacctgaac taaagcaagt
     9001 ggaggaattc gagagtgttt cagaacgtgg agcaaaaggc ttcggaagta gcggagtgta
     9061 aagacatatt agatcgagtc aaggaggttt tggggaagtg agcgacatgt tagaaatatt
     9121 tttaataggg tttggcgttt atctctttta tcgcatagca attatttttc ttaagagtaa
     9181 aaagactata cacacaaaca tatatgaaat gttaatgctt gctactatct ttatgatatc
     9241 tacatttgct tataaacatc aaaagacgca tatcttaata gcatttttag taatgttttt
     9301 tatgagtaag ctcaaacaag ttcaagggag ctatgaggaa tgacacaata cttagtcaca
     9361 acattcaaag attcaacagg acgtaaacat acacacataa ctaaagctaa gagtaatcaa
     9421 aggtttacag ttgttgaggc agagagtaaa gaagaagcga aagagaagta cgagaaacaa
     9481 gttaaaaggg atgcagttat taaagtgggt cagttgtttg aaaatataag ggagtgtggg
     9541 aaatgattaa aaaacttaaa aatatggatg ggttcgacat ctttattgtt ggaatactgt
     9601 cattattcgg tataaccgca ttgctacttg ttgtcgcatt gcctatctat acagtggcta
     9661 gttaccaaaa caaagaagta catcaaggga caattacaga taaatataac aaaagacaag
     9721 ataaagagga caaattctat attgtattag atgataaaca agtcatcgaa aactcagact
     9781 tattcttcaa aggaaagttt gatagcgcag acatacaagc taggttaaaa gtaggtgata
     9841 aagtaaaagt taagacgatt ggatatagaa tacacttttt aaatttatat ccggtcttat
     9901 acgaagtaaa gaaggtagat aaaaaatgat taagcaaata ctaagattat tattcttact
     9961 agcgatgtat gagttaggta agtatgtaac tgagcaagta tatattatga tgacggctaa
    10021 tgatgatgta gaggcgccga gtgattacgt ctttcgagcg gaggtaagtg agtgatgtgg
    10081 attactatga ctattgtatt tgctatattg ctattagttt gtatcagtat taatagtgat
    10141 cgtgcaaggg agatacaagc gctcagatat atgaatgatt atctacttga tgaagtagtt
    10201 aaaactaaag gatacaacgg gttaaaagaa tacaggattg aattaaagcg aatgaataac
    10261 gatattaaaa agtaatttat attatcggag gtattacatt gaatgataaa gattgagaaa
    10321 cacgatatca aaaagcttga agaatacatt cagcacatcg ataactatcg aagagagttg
    10381 aagatgcgag aatatgaatt acttgaaagt catgaaccag ataatgcggg agctggcaaa
    10441 agtaatttgc cgggtaaccc gattgaacga tgtgcaataa agaagtttag tgataacagg
    10501 tacaatacat taagaaatat agttaacggt gtagatagat tgatagatga aagtgatgag
    10561 gatacgcttg agttattaag gtttagatat tgggattgtc ctattggttg ttatgaatgg
    10621 gaagatatag cacattactt tggtacaagt aagacaagta tattacgtag aaggaatgca
    10681 ctgatcgata agttagcaaa gtatattggt tatgtgtagc ggacttttac cctatgtaag
    10741 tccgcattaa aacagtttat tatgttagta tcagattaat atttaaagtt attaaatgct
    10801 aatacgacgc atgaacaaga ggcgcatcac tatgtgatgt gtctttttat ttatgaggta
    10861 tgaacatgtt caaactaata gtaaatacat tactacacat caagtataga tgcgtcttga
    10921 tactacttaa gttatataag gtgaaacatt atgatgacta aagacgaacg catacgattc
    10981 tataagtcta aagaatggca aacaacaaga aaaagagtgc tagaaagaga taattatgaa
    11041 tgtcaacaat gtaagagaga cggcaagtta acgacatatg acaaaagcaa gcgtaagtcg
    11101 ttggatgtag atcatatatt atcgctagaa catcatccgg agtttgctca tgacttaaac
    11161 aatttagaaa cactgtgtat taaatgtcac aacaaaaaag aaaagagatt tataaaaaaa
    11221 gaaaataaat ggaaagacga aaaatggtaa atacccccgg gtcaaaaaaa tcaaaagcga
    11281 tcaaaatact tggggaacgg ggaggggctc gacttcgcga taattttaaa aatccatgta
    11341 taccccccct cttataacca ttttaaggca ggtgatgaaa tggagattat agttgatgaa
    11401 aacttagtgc ttaaagaaaa agaaaggctg caagtattat ataaagacat acctagcaat
    11461 aaattaaaag tagttgatgg tttaattatt caagcagcaa ggctacgtgt aatgcttgat
    11521 tacatgtggg aagacataaa agaaaaaggt gactatgatt tatttactca atctgaaaag
    11581 gcgccaccat atgaaaggga aagaccagta gccaaactat ttaatgctag agatgctgca
    11641 tatcaaaaaa taatcaaaca attatcggat ttattgcccg aagagaaaga agacacagaa
    11701 acgccatctg atgattacct atgattagta ataaatacgt tgatgaatat ataaatttgt
    11761 ggaaacaagg aaagataatt ttaaataaag aaagaattga tctctttaat tatctacaaa
    11821 aacatatata ttcacgagat gatgtatatt ttgatgaaca gaaaatcgag gattgtatca
    11881 aatttattga aaaatggtat tttccaacat taccatttca aaggtttatc atagctaata
    11941 tatttcttat agataaaaat acagatgaag ctttctttac agaatttgct attttcatgg
    12001 gacgtggagg cgggaaaaac ggtctaataa gtgctattag tgattttctt tctacgccct
    12061 tacacggagt taaagaatat cacatctcca ttgttgctaa tagtgaagat caagcaaaaa
    12121 catcgtttga tgaaatcaga accgttttaa tggataacaa acgaaataag acgggtaaaa
    12181 cgccaaaagc tccttatgaa gttagtaaag caaaaataat aaaccgtgca actaaatcgg
    12241 ttattcgata taacacatca aacacaaaaa ccaaagacgg tggacgtgag gggtgtgtta
    12301 tttttgatga aattcattat ttctttggtc ctgaaatggt aaacgtcaaa cgtggtggat
    12361 taggtaaaaa gaaaaataga agaacgtttt atataagtac tgatggtttt gttagagagg
    12421 gttatatcga tgcaatgaag cacaaaattg caagtgtatt aagtggcaag gttaaaaata
    12481 gtagattgtt tgctttttat tgtaagttag acgatccaaa agaagttgat gacagacaga
    12541 cgtgggaaaa ggcgaaccca atgttacata aaccgttatc agaatacgct aaaacactgc
    12601 taagtacgat tgaagaagaa tataacgatt taccattcaa ccgttcaaat aagcccgaat
    12661 tcatgactaa gcgaatgaat ttgcctgaag ttgaccttga aaaagtaata gcaccatgga
    12721 aagaaatact agcgactaat agagagatac caaatttaga taatcaaatg tgtattggtg
    12781 gtttagactt tgcaaacatt cgagattttg caagtgtagg gctattattc cgaaaaaacg
    12841 atgattacat ttggttagga cattcgtttg taagacaagg gtttttggat gatgtcaaat
    12901 tagaacctcc tattaaagaa tgggaaaaaa tgggattatt gaccattgtc gatgatgatg
    12961 tcattgaaat tgaatatata gttgattggt ttttaaaggc tagagaaaaa tatgggcttg
    13021 aaaaagtcat agctgataat tatagaactg atattgtaag acgtgcgttt gaggatgctg
    13081 gcataaaact tgaagtactt agaaatccaa aagcaataca tggattactt gcaccacgta
    13141 tcgatacaat gtttgcgaaa cataacgtaa tatatggaga caatcctttg atgcgttggt
    13201 ttactaataa tgttgcagta aaggttaaac ccgatggtaa taaagaatat attaaaaaag
    13261 atgaaaatag aagaaaaacc gatgggttca tggcttttgt tcacgcatta tatagagcag
    13321 acgatatagt agacaaagac atgtctaaag cgcttgatgc attaatgagt atagatttct
    13381 aatagaggag gtgagacatg agtattctag aaaagatatt taaaactagg aaagatataa
    13441 catatatgct tgatttagat atgatagaag atctatcaca acaagcgtat gtgaaacgtt
    13501 tagcgattga tagttgtatt gaatttgttg cgcgagctgt cgctcaaagt cattttaaag
    13561 tattggaagg taatagaatt caaaagaatg atgtttacta caagttaaat ataaaaccaa
    13621 atactgactt atcaagcgat agtttttggc aacaagttat atataaacta atttatgata
    13681 acgaggtttt aatcgtagta agtgacagca aagaattact tatcgcagat agcttttaca
    13741 gagaagagta cgctttgtat gatgatatat tcaaagatgt aacggttaaa gattatactt
    13801 atcaacgtac tttcacaatg caagaggtca tatatttaaa gtacaacaac aataaagtga
    13861 cacactttgt agaaagtcta ttcgaagatt acgggaaaat attcggaaga atgataggtg
    13921 cacaattaaa aaactatcaa ataagaggga ttttgaaatc tgcctctagc gcatatgacg
    13981 aaaagaatat agaaaaatta caagcgttca caaataaatt attcaatact tttaataaaa
    14041 atcaactagc aatcgcgcct ttgatagaag gttttgatta tgaggaatta tctaatggtg
    14101 gtaagaatag taacatgcct ttttctgaat tgagtgagct aatgagagat gcaataaaaa
    14161 atgttgcgtt gatgattggt atacctccag gtttgattta cggagaaaca gctgatttgg
    14221 aaaagaacac gcttgtattt gagaagttct gtttaacacc tttattaaaa aagattcaga
    14281 acgaattaaa cgcgaaactc ataacacaaa gcatgtattt gaaagataca agaatagaaa
    14341 ttgtcggtgt gaataaaaaa gacccacttc aatatgctga agcaattgac aaacttgtaa
    14401 gttctggttc atttacaagg aatgaggtgc ggattatgtt aggtgaagaa ccatcagaca
    14461 atcctgaatt agacgaatac ctgattacta aaaactacga aaaagctaac agtggtgaaa
    14521 atgatgaaaa agaaaaagat gaaaacactt tgaaaggtgg tgatgaagat gaaagcggag
    14581 attaaaggcg tcatcgtttc caacgaagat aaatgggttt acgaaatgct tggtatggat
    14641 tcgacttgtc ctaaagatgt tttaacacaa ctagaattta gtgatgaaga tgttgatatt
    14701 ataattaact caaatggtgg taacctagta gctggtagtg aaatatatac acatttaaga
    14761 gctcataaag gcaaagtgaa tgttcgcatc acagcaatag cagcaagtgc ggcatcgctt
    14821 atcgcaatgg ctggtgacca catcgaaatg agtccggttg ctagaatgat gattcacaat
    14881 ccttcaagta ttgcgcaagg agaagcgaaa gatctaaatc atgctgcaga aacattagaa
    14941 catgttggtc aaataatggc tgaggcatat gcggttagag ctggtaaaaa caaacaagaa
    15001 cttgtagaaa tgatggctag ggaaacgtgg ctaaatgctg atgaagccat tgaacaaggt
    15061 tttgcggata gtaaaatgtt tgaaaacgac aatatgcaaa ttgtagcaag caatacacaa
    15121 gtgttatcga aagatgtatt aaatcgtgta acagctttgg taagtaaaac gccagaggtt
    15181 aacattgata ttgacgcaat agcaaataaa gtaattgaaa aaataaatat gaaagaaaag
    15241 gaatcagaaa tcgatgttgc agatagtaaa gtatcagcaa atggattttc aagattcctt
    15301 ttttaataca aaaaatagga ggtcataaaa tgactataaa tttatcggaa acattcgcaa
    15361 atgcgaaaaa cgaatttatt aatgcagtaa acaacggtga accgcaagaa agacaaaatg
    15421 aattgtacgg tgacatgatt aaccaactat ttgaagaaac taaattacaa gcaaaagcag
    15481 aagctgaaag agtttctagt ttacctaaat cagcacaatc tttgagtgca aaccaaagaa
    15541 gtttcttcat ggatatcaat aaaaacgtta actataaaga agaaaaactt ttgccagaag
    15601 aaacaattga tagaattttt gaagatttga cgacgaatca tccgttatta gctgatttag
    15661 gtattaaaaa cgctggtttg cgtttgaagt tcttaaaatc tgaaacttct ggcgtagccg
    15721 tttggggtaa aatctatggt gaaattaaag gtcaattaga tgctgcgttc agtgaagaaa
    15781 cagcaattca aaataaattg acagcgtttg ttgttttacc aaaagattta aatgattttg
    15841 gtcctgcgtg gattgaaaga tttgttcgtg ttcaaatcga agaagcattt gcagtggcgc
    15901 ttgaaactgc gttcttaaaa ggtactggta aagaccaacc aatcggctta aaccgtcaag
    15961 tacaaaaagg tgtatcggta actgagggtg cttatccaga gaaagaagaa caaggtacgc
    16021 ttacatttgc taatccgcgc gctacagtta atgaattgac gcaagtgttt aaataccact
    16081 caactaacga gaaaggtaaa tcagtagcgg ttaaaggtaa tgtaacaatg gttgttaatc
    16141 cgtccgatgc ttttgaggtt caagcacagt atacacattt aaatgcaaat ggcgtatatg
    16201 ttactgcttt accatttaat ttgaatgtta tcgagtctac agtccaagaa gcaggtaagg
    16261 ttttaacgta cgttaaaggt ttatatgatg gttatttagc tggtggtatt aatgttcaga
    16321 aatttaaaga aacacttgcg ttagatgata tggatttata cactgcaaaa caatttgctt
    16381 acggcaaagc gaaagataat aaagttgctg ctgtttggaa attagattta aaaggacata
    16441 agccagcttt agaaggtacc gaagaaacac tataaaattt tatgaggtga taaaatggtg
    16501 aaatttaaag ttgttagagc ttttaaagac atagagcaca atcaacacaa gtacaaagta
    16561 ggggagttgt atccagctga agggtataac aatcctcgtg ttgaattgtt gacaaatcaa
    16621 atcaaaaata agtacgacaa agtttatatc gtacctttag ataagctgac aaaacaagaa
    16681 ttattagaac tatgcgaatc attacaaaaa aaagcgtcta gttcaatggt taaaagtgaa
    16741 atcgtcgact tattgaatgg tgaagacaat gacgattgat gatttgcttg tcaaatttaa
    16801 atcacttgaa aagattgacc ataattcaga ggatgagtac ttaaagcagt tgttaaaaat
    16861 gtcgtacgag cgtataaaaa atcagtgcgg agtttttgaa ttagagaatt taataggtca
    16921 agaattgata cttatacgcg ctagatatgc ttatcaagat ttattagaac acttcaacga
    16981 taattacaga cctgaaataa tagatttttc gttatctcta atggaggtat cagaagatga
    17041 agaaagtgtt taaaaaacct agaattacaa ctaaacgttt aaatactcgt gttcattttt
    17101 ataagtatac tgaaaataat ggtccagaag ctggagaaaa agaagaaaaa ttattatata
    17161 gctgttgggc gagtattgat ggtgtctggt tacgtgaatt agaacaagct atctcaaacg
    17221 gaacccaaaa tgacattaaa ttgtatattc gtgatccgca aggtgattat ttacccagtg
    17281 aagaacatta tcttgaaatt gaatcaagat atttcaaaaa tcgtttgaat ataaagcaag
    17341 tatcaccaga tttggataat aaagacttta ttatgattcg tggaggatat agttcatgag
    17401 tgtgaaagtg ataggtgata aagcattaga aagagaatta gaaaatcgtt tcggaactca
    17461 agcgatgtta aaagttcaag atagggcgct aattgctggt gctaaagtaa ttgttgaaga
    17521 agtaaaaaaa caactaaagc catcaaaaga tacgggcgca ttaattaatg aggtaagttt
    17581 tagtaaacct gaatggataa acggaaaacg tacaattact gttcattggc gaggttctaa
    17641 agaccgttat aaaatcgtac atttaattga aaatggacac gttcaaaaag ggacaggtaa
    17701 atttatcaaa cctaaagcta tgggtggcgt taatagagca ataagacaag ggcaaaataa
    17761 gtattttgag acgctaaaaa gggagttaaa aaaattgtga ttgatatttt gtacaaagtt
    17821 catgaagtga ttagtcaaga cagaattatt agagagcacg taaatatcaa taatattaag
    17881 ttcaataaat acccaaacgt aaaagatacg gatgtacctt tcatagttat tgacgatata
    17941 gacgacccaa tacctacaac ttatactgac ggagatgagc gcgcatatag ttacattgta
    18001 caaatagatg tatttgtgaa gtttaacgaa aagtataacg cgagaatcat aagaaataag
    18061 atatctaatc gtattcaaaa gttattatgg tctgaactga aaatgggaaa tgtatccaac
    18121 ggaaaacctg aatatataga agaatttaaa acatatagaa gcactcgtgt ctacgagggc
    18181 attttttacg aggaggaata ataaatggta gtaaaacaag caagtgcgcc gaaagcgtat
    18241 tttaacatta ctggtttagg tttcgctaaa ttaacaaaag aaggcgcaaa gctagaatat
    18301 agcgatatta caaaaactag aggattacaa aaaatcggtg ttgaaactgg aggagacttg
    18361 aaaacagcat atgctgatgg tggtccaatc gaatcaggga acacagacgg agaaggtaaa
    18421 atttcgttac aaatgcatgc tttccctaaa gagattcgca aaatagtatt caatgaagag
    18481 tatgatgaat acggtgttta taaagaaact caaggtaaac aaaacaacta tgtagcaatt
    18541 tggttcagac aagagcgtcg agacggcaca tttagaacgg ttttattacc taaagtcatg
    18601 tttacaaatc ctaaaatcga tggcgaaacg gctgagaaag attgggattt ctcaagcgaa
    18661 gaggttgaag gtgaggcact tttcccttta attgataata aaaagtcagt acgtaaatat
    18721 atctttgatt cagcaaacat gacaaatcat agtggaaatg gtgaaaaagg cgaagaggct
    18781 ttcttaaaga aaattttagg cgaagaatat actggaaacg tgacagagga taacggcgaa
    18841 actttgtaac gcaaccggct tcatcggaaa ctgagataaa gtcggttaat ataccagata
    18901 gttttaaaac actaaaagtt ggcaacacat atgatttaaa tgttgtagta gagccatcta
    18961 atcaaagtaa gttattgaaa tacacaacag atcaaacgaa tattgtatca atcaatagtg
    19021 atggtcaagt cactgcggaa gcacaaggta ttgccacggt taaagcatat gttggtaata
    19081 tgagtgacac tataacaata aatgtagaag cataagaggg gacaacccct ctattttatt
    19141 tgaaaataag gagagtatta taaaatggca aaattaaaac gtaacattat tcaattagta
    19201 gaagatccaa aagcaaatga aattaaatta caaacgtact taacaccaca cttcatttca
    19261 tttgaaattg tatacgaagc aatggattta atcgatgata ttgaggacga aaatagcacg
    19321 atgaagccaa gagaaatcgc tgacagattg atggatatgg ttgtaaaaat ttacgataac
    19381 caattcgcag ttaaagacct aaaagaacgt atgcatgcac ctgatggaat gaatgcactt
    19441 cgtgaacaag tggttttcat tacgcaaggt caacaaactg aggaaactag aaattttatc
    19501 cagaacatga aataaagcct gaagatttaa catataaagc aatgttgaaa aatatggata
    19561 ctctcatgat ggacttaatt gaaaatggta aagacgctaa cgaagtttta aaaatgccat
    19621 ttcattatgt gctttccata tatcaaaata aaaacaatga catttctgaa gaaaaagcag
    19681 aggctttaat tgatgcattt taaccttaac cgtttggtta gggttatttt tttgaacttt
    19741 tttagaaagg aggtaaaaaa tgggagaaag aataaaaggt ttatctatag gtttggattt
    19801 agatgcagca aatttaaata gatcatttgc agaaatcaaa cgaaacttta aaactttaaa
    19861 ttctgactta aaattaacag gcaacaactt caaatatacc gaaaaatcaa ctgatagtta
    19921 caaacaaagg attaaagaac ttgatggaac tatcacaggt tataagaaaa acgttgatga
    19981 tttagctaag caatatgaca aggtatctca agaacagggc gaaaacagtg caggagctca
    20041 aaagttacga caagaatata acaaacaagc aaatgaactg aattatttag aaagagaatt
    20101 acaaaaaaca tcagccgaat ttgaagagtt caaaaaagct caagttgaag ctcaaagaat
    20161 ggcagaaagt ggctggggaa aaaccagtaa agtttttgaa agtatgggac ctaaattaac
    20221 aaaaatgggt gatggtttaa aatccattgg taaaggcatg atgattggtg taactgcacc
    20281 tgttttaggt atcgcagcag catcaggaaa agcttttgca gaagttgata aaggtttaga
    20341 tactgttact caagcaacag gcgcaacagg cagtgaatta aaaaaattgc agaactcatt
    20401 taaagatgtt tatggcaatt ttccagcaga tgctgaaact gttggtggag ttttaggaga
    20461 agttaataca agattaggtt ttacaggtaa agaacttgaa aatgccacag agtcattctt
    20521 gaaattcagt catataacag gttctgacgg tgtgcaagcc gtacagttaa ttacccgtgc
    20581 aatgggcgat gcaggtatcg aagcaagtga atatcaaagt gttttggata tggtagcaaa
    20641 agcggcgcaa gctagtggga taagtgttga cacattagct gatagtatta ctaaatacgg
    20701 cgctccaatg agagctatgg gctttgagat gaaagaatca attgctttat tctctcaatg
    20761 ggaaaagtca ggcgttaata ctgaaatagc attcagtggt ttgaaaaaag ctatatcaaa
    20821 ttggggtaaa gctggtaaaa acccaagaga agaatttaag aagacattag cagaaattga
    20881 aaagacgccg gatatagcta gcgcaactat tttagcgatt gaagcatttg gtgcaaaggc
    20941 aggtcctgat ttagcagacg ctattaaagg tggtcgcttt agttatcaag aatttttaaa
    21001 aactattgaa gattcccaag gcacagtaaa ccaaacattt aaagattctg aaagtggctc
    21061 cgaaagattt aaagtagcaa tgaataaatt aaaattagta ggtgctgatg tatgggcttc
    21121 tattgaaagt gcgtttgctc cagtcatgga agaattaata aaaaagctat cagtcgctgt
    21181 cgattggttt tcgaatttaa gtgatggttc taaaagatca attgttattt tcggtggtat
    21241 tgctgctgca attggtcctg tagtttttgg gttaggtgca tttataagca caattggtaa
    21301 tgcagtaact gtattagccc cactattagc tggtattgca aaggctgatg gattaattag
    21361 ttttttatcg actaaagtac ctatattagg aactgtcttc acagctttaa ctggtccaat
    21421 tggcattgta ttaggtgttt tagctggcct agcagtcgca tttacaattg cttataagaa
    21481 atctgaaaca tttagaaatt ttgttaatga tgcaattgaa agtgttaaac aaacatttag
    21541 taattttatt caatttattc aacctttcat tgattccgtt aaaaacgtct ttaaacaagc
    21601 ggtttcagca atcgttgatt tcgctaaaga tatttggagt caaattaatg gattctttaa
    21661 tgaaaacgga atttctattg ttcaagcgct tcaaaatata tgcaatttta tcaaagttat
    21721 atttgaattt atcttaaatt ttgtaattaa accaataatg tttgcaatct ggcaagtgat
    21781 gcaatttatt tggccggcgg ttaaagcctt gattgtcagt acttgggaga atataaaagg
    21841 tgtgatacaa ggaactttaa atatcatact aggtttaatt aagttcttct caagtttatt
    21901 tactggagat tggcgaggag tttgggacgc gattattatg attcttaaag gagtcgttca
    21961 attaatatgg aatttaattc aattatggtt tgtaggcaaa atacttggcg ttgttaggta
    22021 ctttggcgga ttgctaaaag gattaataac aggtatttgg gacgtaataa aaagtatatt
    22081 cagtaaatct ttatcagcaa tttggaatgc gacaaaaagt atttttggat tcttatttaa
    22141 tagtgtcaaa tcaattttca cgaatatgaa aaattggtta tctaacacat ggagtagtat
    22201 ccgtacgaat acgataggaa aagcacagtc attatttagt ggtgtgaaat caaaattcac
    22261 tagtttgtgg aatgcgacaa aagatatttt tagtaatcta agaaattgga tggcaaatat
    22321 ttggaattcc attaaagata atacggttgg aattgcaagt cgtttatgga gtaaagtgcg
    22381 tggaattttt acaaatatgc gtgacggctt acaaagtatt atcagcaaaa ttaaaagtca
    22441 tatcggcggt atggtagatg ctattaaaaa aggacttaat aaattaattg aaggtttaaa
    22501 ctgggtcggt ggtaagctgg gaatggataa aatacctaag ttacacactg gtacagaaca
    22561 cacacatact actacaagat tagttaagaa cggtaagatt gcgcgggata cgttcgctac
    22621 ggttggggat aaaggacgtg gaaatggtcc gaatggtttc agaaatgaaa taattgaatt
    22681 ccctaatggt aaacgtgtaa tcacacctaa tacagatact accgcttatt tacctaaagg
    22741 ctcaaaagta tacaacggta cacaaactta ttcaatgtta aacggaacgc ttccaagatt
    22801 tagcttaggt actatgtgga aagatattaa atccggtgca tcatcggcat ttaactggac
    22861 aaaagatcaa ataggtaaag gtaccaaatg gcttggcgat aaagttggcg atgttttaga
    22921 ttttatggaa aatcctggca aacttttaaa ttatatactt gaagcttttg gaattgattt
    22981 caattcttta actaaaggta tgggaattgc aggcgacata acaaaagctg catggtctaa
    23041 gattaagaaa agtgctactg attggataaa agaaaattta gaagctatgg gcggtggcga
    23101 tttagtcggc ggaatattag accctgacaa aattaattat cattatggac gtaccgcagc
    23161 ttatactgct gcaactggaa gaccatttca tgaaggtgtc gattttccat ttgtatatca
    23221 agaagttaga acgccgatgg gtggcagact tacaagaatg ccatttatgt ctggtggtta
    23281 tggtaattat gtaaaaatta ctagtggagt tatcgatatg ctatttgcgc atttgaaaaa
    23341 ctttagcaaa tcaccaccta gtggcacgat ggtaaagccc ggcgatgttg ttggtttaac
    23401 tggtaatacc ggatttagta caggaccaca cttacatttt gaaatgagaa gaaatggacg
    23461 tcactttgac cctgaaccat atttaagaaa tgcaaagaaa aaaggtaggt tatcaattgg
    23521 tggcggtggt gctacttctg gaagtggtgc aacttatgca agccgagtaa tccgtcaagc
    23581 gcaaagtatt ttaggaggac gttataaagg taagtggatt catgaccaga tgatgcgagt
    23641 tgcaaagcgt gaaagtaact atcaatcaaa tgcagtgaat aattgggata ttaatgctca
    23701 aagaggagac ccatcaagag gattattcca aatcatcggc tcaactttta gagcaaacgc
    23761 taaacgtgga tatactaact ttaataatcc ggttcatcaa ggtatttcag caatgcagta
    23821 cattgttaga cgctacggtt gggatggatt taagcgtgct ggtgattacg catatgctac
    23881 aggtggaaaa gtttttgatg gttggtataa cttaggtgaa gacggtcatc cagaatggat
    23941 tattccaaca gatccagctc gtagaaatga tgcaatgaag atgttgcatt atgcagcagc
    24001 agaagtaaga gggagaaaag cgagtaaaaa taagcgtcct agtcaattgt ctagtgtaaa
    24061 tgggtttgat gacccaagct tattattgaa aatgattgaa caacagcaac aacaaatagc
    24121 tttattactg aaaatagcgc aatccaacga tgtgattgca gataaagatt atcagccgat
    24181 tattgacgaa tacgcttttg ataaaaaggt gaacgcgtct atagaaaagc gagaaaggca
    24241 agaatcaaca aaagtaaagt ttagaaaagg aggaattgct attcaatgat agacactatt
    24301 aaagtgaaca acaaaacaat tccttggttg tatgtcgaaa gagggtttga aataccctct
    24361 tttaattatg ttttaaaaac tgaaaatgta gatggacgtt cggggtctat atataaaggg
    24421 cgtaggcttg aatcttatag ttttgatata cctttggtgg tacgtaatga ctatttatct
    24481 cacaacggct ttaaaacaca tgatgacgtc ttgaatgaat tagtaaagtt ttttaactac
    24541 gaggaacaag ttaaattaca attcaaatct aaagattggt actggaacgc ttattttgaa
    24601 ggaccaataa agctgcacaa agaatttgca atacctgtta agttcactat caaagtagta
    24661 ctaacagacc cttacaaata ttcagtaaca ggatataaaa atactgcgat ttcagaccaa
    24721 gtttcagttg taaatagtgg gactgctgac actcctttaa ttgttgaagc ccgagcaatt
    24781 aaaccatcta gttactttat gatcactaaa aatgatgaag attattttat ggttggtgat
    24841 gatgaggtaa ccaaagaagt taaggattac atgcctcctg tttatcatag tgagtttcgt
    24901 gatttcaaag gttggactaa gatgattact gaagatattc caagtaatga tttaggtggt
    24961 aaagtcggcg gtgactttgt gatatccaat cttggcgaag gatataaagc aactaatttt
    25021 tctgatgcaa aaggttgggt tggtgctggc acgaaacgag ggctccctaa agcgatgaca
    25081 gattttcaaa ttacctataa atgtattgtt gaacaaaaag gtaaaggtgc cggaagaaca
    25141 gcacaacata tttatgatag tgatggtaag ttacttgctt ctattggtta tgaaaataaa
    25201 tatcatgata gaaaaatagg acatattgtt gttacgttgt ataaccaaaa aggagacccc
    25261 aaaaagatat acgactatca gaataaaccg ataatgtata acttggacag aatcgttgtt
    25321 tatatgcggc tcagaagagt aggtaataaa ttttctatta aaacttggaa atttgatcac
    25381 attaaagacc cagatagacg taaacctatt gatatggatg agaaagagtg gatagatggc
    25441 ggtaagtttt atcagcgtcc agcttctatc atagctatct atagtgcgaa gtataatggt
    25501 tataagtgga tggagatgaa tggattaggt tcattcaata cggagattct accaaaaccg
    25561 aaaggcgcaa gggatgtcat tatacaaaaa ggtgatttag taaaaataga tatgcaagca
    25621 aaaagtgttg tcatcaatga ggaaccaatg ttgagcgaga aatcgtttgg aagtaattat
    25681 ttcaatgttg attctgggta cagtgaatta atcatacaac ctgaaaacgt ctttgatacg
    25741 acggttaaat ggcaagatag atatttatag aaaggagatg agagtgtgat acatgtttta
    25801 gattttaacg acaagattat agatttcctt tctactgatg acccttcctt agttagagcg
    25861 attcataaac gtaatgttaa tgacaattca gaaatgcttg aactgctcat atcatcagaa
    25921 agagctgaaa agttccgtga acgacatcgt gttattataa gggattcaaa caaacaatgg
    25981 cgtgaattta ttattaactg ggttcaagat acgatggacg gctacacaga gatagaatgt
    26041 atagcgtctt atcttgctga tataacaaca gctaaaccgt atgcaccagg caaatttgag
    26101 aaaaagacaa cttcagaagc attgaaagat gtgttgagcg atacaggttg ggaagtttct
    26161 gaacaaaccg aatacgatgg cttacgtact acgtcatgga cttcttatca aactagatat
    26221 gaagttttaa agcaattatg tacaacctat aaaatggtat tggattttta tatagagctt
    26281 agttctaata ccgtcaaagg tagatatgtg gtactcaaaa agaaaaacag cttattcaaa
    26341 ggtaaagaaa ttgagtatgg taaagatttg gttgggttaa ctaggaagat tgatatgtca
    26401 gaaatcaaaa cagcattaat tgctgtggga cccgaaaatg acaaaggaaa gcgtttagag
    26461 ctagttgtga cagatgacga agcgcaaagt caattcaacc tacctatgcg ctatatttgg
    26521 gggatatatg aaccacaatc agatgatcaa aatatgaatg aaacacgatt aagttcttta
    26581 gccaaaacag agttaaataa acgtaagtcg gcagttatgt catatgagat tacttctact
    26641 gatttggaag ttacgtatcc gcacgagatt atatcaattg gcgatacagt cagagtaaaa
    26701 catagagatt ttaacccgcc attgtatgta gaggcagaag ttattgctga agaatataac
    26761 ataatttcag aaaatagcac atatacattc ggtcaaccta aagagttcaa agaatcagaa
    26821 ttacgagaag agtttaacaa gcgattaaac ctaatacacc aaaaattaaa cgacaatatt
    26881 agcaatatca atactatagt aaaagatgtt gtagatggtg aattagaata ctttgaacgc
    26941 aaaattcata aaagtgatac accgccagaa aatccagtca atgatacgct ttggtatgat
    27001 acaagtaacc ctgatgttgc tgtcttgcgt agatattgga atggtcgatg gattgaagca
    27061 acaccaaatg atgttgaaaa attaggtggt ataacaagag agaaagcgct attcagtgaa
    27121 ttaaacaata tttttattaa tttatctata caacacgcta gtcttttgtc agaagctaca
    27181 gaattactga atagcgagta cttagtagat aatgatttga aagcggactt acaagcaagt
    27241 ttagacgctg tgattgatgt ttataatcaa attaaaaata atttagaatc tatgacaccc
    27301 gaaactgcaa cgattggtcg gttggtagat acaaaaactt tatttcttga gtatagaaag
    27361 aaattacaag atgtttatac agatgtagaa gatgtcaaaa tcgccatttc agatagattt
    27421 aaattattac agtcacaata cactgatgaa aaatataaag aagcgttgga aataatagca
    27481 acaaaatttg gtttaacggt gaatgaagat ttgcagttag tcggagaacc taatgttgtt
    27541 aaatcagcta ttgaagcagc tagagaatcc acaaaagaac aattacgtga ctatgtaaaa
    27601 acatcggact ataaaacaga caaagacggt attgttgaac gtttagatac tgctgaagct
    27661 gagagaacga ctttaaaagg tgaaatcaaa gataaagtta cgttaaacga atatcgaaac
    27721 ggattggaag aacaaaaaca atatactgat gaccagttaa gtgatttgtc caataatcct
    27781 gagattaaag caagtattga acaagcaaat caagaagcgc aagaagcttt aaaatcatac
    27841 attgatgctc aagataatct taaagagaag gaatcgcaag cgtatgctga tggtaaaatt
    27901 tcggaagaag agcaacgcgc tatacaagat gctcaagcta aacttgaaga ggcaaaacaa
    27961 aacgcagaac taaaggctag aaacgctgaa aagaaagcta atgcttatac agacaacaag
    28021 gtcaaagaaa gcacagatgc acagaggaga acactgactc gctatggttc tcaaattata
    28081 caaaatggta aggaaatcaa attaagaact actaaagaag agtttaatgc aaccaatcgt
    28141 acactttcaa atatattaaa cgagattgtc caaaacgtta cagatggaac aacaatcaga
    28201 tatgatgata acggagtggc tcaagcttta aatgtggggc cacgtggtat tagattaaat
    28261 gctgataaaa ttgatattaa cggtaataga gaaataaacc ttcttatcca aaatatgcga
    28321 gataaagtag ataaaaccga tattgtcaac agccttaatt tatcaagaga gggtcttgat
    28381 atcaatgtta atagaattgg aattaaaggc ggtaacaata acagatatgt tcaaatacag
    28441 aatgattcta ttgaactagg tggtattgtg caacgaactt ggaaaggcaa acgatcaacc
    28501 gatgatatat tcacacgtct taaagatgga catctaaggt ttagaaataa taccgcaggc
    28561 ggttcacttt atatgtcaca ttttggtatt tcaacatata ttgatggaga aggcgaagac
    28621 ggaggttcat ccggtactat tcaatggtgg gataaaactt acagtgatag cggtatgaat
    28681 ggcataacaa tcaattccta tggtggtgtc gttgcactaa cgtcagataa taatcgggtt
    28741 gttctggagt cttacgcttc atcgaatatc aaaagcaaac aggcaccggt gtatttatat
    28801 ccaaacacag acaaagtgcc tggattaaac cgatttgcat tcacgctgtc taatgcagat
    28861 aatgcttatt cgagtgacgg ttatattatg tttggttctg atgagaacta tgattacggt
    28921 gcgggtatca ggttttctaa agaaagaaat aaaggtcttg ttcaaattgt taatggacga
    28981 tatgcaacag gtggagatac aacaatcgaa gcagggtatg gcaaatttaa tatgctgaaa
    29041 cgacgtgatg gtaataggta tattcatata cagagtacag acctactgtc tgtaggttca
    29101 gatgatgcag gagataggat agcttctaac tcaatttata gacgtactta ttcggccgca
    29161 gctaatttgc atattacttc tgctggcaca attgggcgtt cgacatcagc gcgtaaatac
    29221 aagttatcta tcgaaaatca atataacgat agagatgaac aactggaaca ttcaaaagct
    29281 attcttaact tacctattag aacgtggttt gataaagctg agtctgaaat tttagctaga
    29341 gagctgagag aagatagaaa attatcggaa gacacctata aacttgatag atacgtaggt
    29401 ttgattgctg aagaggtgga gaatttagga ttaaaagagt ttgtcacgta tgatgacaaa
    29461 ggagaaattg aaggtatagc gtatgatcgt ctatggattc atcttatccc tgttatcaaa
    29521 gaacaacaac taagaatcaa gaaattggag gagtcaaaga atgcaggata acaaacaagg
    29581 attacaagct aatcctgaat atacaattca ttatttatca caggaaatta tgaggttaac
    29641 acaagaaaac gcgatgttaa aagcgtatat acaagaaaat aaagaaaatc aacaatgtgc
    29701 tgaggaagag taatctttag cactattttt atacaaaaat ttaaggaggt catttaatta
    29761 tggcaaaaga aattatcaac aatacagaaa ggtttatttt agtacaaatc gacaaagaag
    29821 gtacagaacg tgtagtatat caagatttca caggaagttt tacaacttct gaaatggtta
    29881 accatgctca agattttaaa tctgaagaaa acgctaagaa aattgcggag acgttaaatt
    29941 tgttatatca attaactaac aaaaaacaac gtgtgaaagt agttaaagaa gtagttgaaa
    30001 gatcagattt atctccagag gtaacagtta acactgaaac agtatgaaaa gctatgagtt
    30061 agatactcat aatctttatt cttttagaaa gcgggtgtac tgaattgggg tggttcaaaa
    30121 aacacgaaca tgaatggcgc atcagaaggt tagaagagaa tgataaaaca atgctcagca
    30181 cactcaatga aattaaatta ggtcaaaaaa cccaagagca agttaacatt aaattagata
    30241 aaaccttaga tgctattcaa aaagaaagag aaatagatga aaagaataag aaagaaaatg
    30301 ataagaacat acgtgatatg aaaatgtggg tgcttggttt agttgggaca atatttggat
    30361 cgctaattat agcattattg cgtatgctta tgggcatata agagaggtga ataaaatgtt
    30421 taaactaatc tttggttata gtttctggac atgtttttgg ttcggtaaat gtaaataagt
    30481 tttagtcagt gcttcggtac tgacttttta tttattgttg taattatggt aatatgcaga
    30541 agtgagcaag ttggatagat ggtggctatc tgagtataag gaggtggtgc ctatggtggc
    30601 attactgaaa tctttagaaa ggagacgcct aatgattaca attagtacca tgttgcagtt
    30661 tggtttattc cttattgcat tgataggtct agtaatcaag cttattgaat taagcaataa
    30721 aaaataacca tcgctaactt tggctggttt cgatggttaa atggttatta atttaatctt
    30781 taatctaaaa tagccaccgt ctttttaacg ggctcattag ggtaacatgt ttgcgcatgt
    30841 tgcccttttt ctatatataa attaacacac cataatataa atatcaaata gacggcttat
    30901 tagtcgtctt tttattttgg gtaaaaggag ataagaatat gattaattgg aaaattagaa
    30961 tgaaacaaaa atcattttgg gtagcgatat tgtcagctat ctttttattt gctcaaaaca
    31021 tcgcaaaagc tattgggtat gatatccaag tttatacaga gcaattaaca gacggtttaa
    31081 acgctatatt aggattttta gtattaactg gtgtgattca agacccgact actaaaggta
    31141 taggtgatag ccaccaagct ttagaatatg aagaaccaag aagaaaatac taggaggtaa
    31201 aataatgaaa acatacagtg aagcaagagc aaggttacgt tggtatcaag gtagatatat
    31261 tgattttgac ggttggtatg gttaccaatg tgcagattta gcagttgatt acatttattg
    31321 gttgttagaa attagaatgt ggggaaatgc aaaagatgca atcaataacg attttaaaaa
    31381 catggcaaca gtatatgaaa acacaccatc gtttgttcca caaataggtg atgtggctgt
    31441 atttaccaaa ggaatatata aacaatacgg tcatattggt ttagtgttta atggtggtaa
    31501 tacaaaccaa tttttaattt tggaacagaa ctatgacggt aacgcaaata cgcctgcaaa
    31561 gttacgttgg gataattatt acggctgtac tcactttatt agacctaagt ataaaagtga
    31621 gggcttaatg aataagatca caaataaagt taaaccacct gctcaaaaag cagtcggtaa
    31681 atctgcaagt aaaataacag ttggaagtaa agcgccttat aaccttaaat ggtcaaaagg
    31741 tgcttatttt aatgcgaaaa tcgacggctt aggtgctact tcagccacta gatacggtga
    31801 taatcgtact aactatagat tcgatgttgg acaggctgta tacgcgcctg gaacattaat
    31861 atatgtgttt gaaattatag atggttggtg tcgcatttat tggaacaatc ataatgagtg
    31921 gatatggcat gagagattga ttgtgaaaga agtgttttaa ttcttaggtt aaaatgttaa
    31981 atatttgtta attatttttt aatgtaattt tagtttcttt taatatttta ttgattttta
    32041 atattttctc aatataaaat gaagttgttg atatttatca tcttaaataa gggtgttagc
    32101 tataaaaaga gataaataaa aacaaatata ttatatttgg aggaagcgcc atgctcaaaa
    32161 gaggtttatt atttttaact gttttattgt tattattctc attttcttca attactaatg
    32221 aggtaagtgc atcaagttca ttcgacaaag gaaaatataa aaaaggcgat gacgcgagtt
    32281 attttgaacc aacaggcccg tatttgatgg taaatgtgac tggagttgat ggtaaaggaa
    32341 atgaattgct atcccctcat tatgtcgagt ttcctattaa acctgggact acacttacaa
    32401 aagaaaaaat tgaatactat gtcgaatggg cattagatgc gacagcatat aaagagttta
    32461 gagtagttga attagatcca agcgcaaaga tcgaagtcac ttattatgat aagaataaga
    32521 aaaaagaaga aacgaagtct ttccctataa cagaaaaagg ttttgttgtc ccagatttat
    32581 cagagcatat taaaaaccct ggattcaact taattacaaa ggttattata gaaaagaaat
    32641 aaaacaaaat agttgtttat tatagaaagc aatgtcttga ttgaatatgt gtagtgaaaa
    32701 ttatctttca tcaaattctc attcatgcac gaatggttct tccccaccta atcagatatt
    32761 aggtgactta tggggagaaa tcagttagga tgaaaaagtg gataatcctt ttttaggcag
    32821 gtacttcggt acttgcctat ttttttatgt tataatcttt ctagacgtat tcaagggacg
    32881 tctttttaga ttgtatgtta tagctagctt tcgggctagt tttttgttat gatgtgttac
    32941 acatgcatca actatttaca tctatccttg ttcacccaag catgtcactg ggtgtttttt
    33001 cttatgatag agagcatagt tttcatacta ctccctcgta gtatatatga ctttagcatt
    33061 cccgtataat agtttacggg gtgcttttta tgttataatt aactgtatat agtaggagtg
    33121 aactatatag cctgttaagt ggcctagtaa cctaacactt atcctgcaat tgatatcctt
    33181 tttgcccttc actcgataca tatatctcaa caacatagaa atattacagt cgctacaccg
    33241 catcttaaat ggtgtggtta tttttattgg aagtgtgtat caggtatcag taatgttaaa
    33301 acaccagcta aaaatgaaaa gaattcacca gtgccagcag gttatacact cgataaaaac
    33361 aatgtaccgt ataaaaaaga gactggttat tacacagttg ccaatgttaa aggtaataac
    33421 gtgagggatg gctattcaac taattcaaga attacaggtg tattacccaa taacgcaact
    33481 atcaaatatg acggcgcata ttgcattaat ggctatagat ggattactta tattgctaat
    33541 agtggacaac gtcgttatat agcgacagga gaggtagaca aggcaggtaa tagaataagc
    33601 agttttggta agtttagtgc agtttgataa ttagatatat aaaggtttgg caagttatga
    33661 aatgtctgcc aaacctttat ataaaaaaga aatatctacc ttttaattta tgtaactact
    33721 attagtatgc atattcatta gtttttccag gaccattaat tacataagat gatttagact
    33781 ctcctttttt aaagaagtat gttttataca ttttacctag taactcaaca ttttttctat
    33841 cttcagcaag tggtgtattc agatatactg tatagtaacc tttattttca gttaaaataa
    33901 ccattttttc aaattgagca gaattttttg tgcctttctt taaataattt ctcaaacgtt
    33961 catctaattt tcctagcgtt gtaggaagac cactattttt aaatgattct ttataagctt
    34021 tttctttctc taacattttc ttatttgatt ctatttcttc atttgtagga aacggttcaa
    34081 aagtaaaagc tttcgctgaa tgatggtgtg tactgattcc tgccgttaaa aaacttaatg
    34141 ctaaaactgt tgttgctaat ttctttttca taatgatgtt aattctcctt aaattctata
    34201 tttaaattta tagttagttt tgcgaaattc ctaaaatgag tttaat
//
LOCUS       DALAGD010000017        33941 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.017, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000017 DALAGD010000000
VERSION     DALAGD010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 33941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33941
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.017"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            176..1027
                     /locus_tag="O2701_000629"
     CDS             176..1027
                     /locus_tag="O2701_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCZ4292538.1"
                     /translation="MGEYIVTKTLNNNVVVCTNNDQEVILIGKGIGFNKKEGMALNDQ
                     TITIEKIYKLESEQQKAHYKSLVEIADDNVLQVIIDSLNFISNTAMNVDSKQLVVSLT
                     DHIIFAYKRLKQNQVISNPFVMETMQLYSDAYHIAKQVIDQLNAALDVHFPEDEIGFI
                     ALHIASNTEDLSMHEMTLINNVIKKGIDIIESDLVTTVDKESLQYQRFIRHVQFLIRR
                     LRRKEYIHAQDDFVSMIKNHYPICYNTAYKILTMIQKQFDVNISESEIIYLTLHIHHF
                     EERINQS"
     gene            1002..1169
                     /locus_tag="O2701_000630"
     CDS             1002..1169
                     /locus_tag="O2701_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292539.1"
                     /translation="MKKGLINPKLSVLHGRFVTVKGICMVSLMLVILHKWTVFIQHLM
                     NILSGGESIIE"
     gene            1162..2370
                     /locus_tag="O2701_000631"
     CDS             1162..2370
                     /locus_tag="O2701_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCZ4292540.1"
                     /translation="MNENEKNIRKNFLKFTESRYMKFVGGNDLVFSLIALVLLGIVIF
                     IFEKVSYVFDPFIIVFKTIAAPIIVSLILFYLFNPIVNMMERYRIPRVAGISIIYLAV
                     VGVITLIVNLLIPIIGSQVDSLVKNSPQYLEKLINSIDKIANNTFFSSYYSQINDWLN
                     SLPKKIPSMLSEFTDGFGSKIATFAETIANIGVVIVTTPFVLFFMLKDGHHFKEFSTN
                     IMPPKFRKDFHDLLEKMSVQVGSYIQGQIIVSFCIGILLFIGYSVIGLKYSLVLASIA
                     AVTSVVPYLGPTIAISPAIVIAAITSPWMLLKLAVVWTLVQFVEGHFISPNIMGKTLK
                     IHPLTIIFILLCAGKLLGIVGVILGIPGYAILKVLVTHLFQLFKRRYNRFYGNDVGEY
                     DIKESNKIVE"
     gene            2851..5373
                     /gene="mprF"
                     /locus_tag="O2701_000632"
     CDS             2851..5373
                     /gene="mprF"
                     /locus_tag="O2701_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCZ4292541.1"
                     /translation="MNQEVKNKIFSILKITFATALFIFVAITLYRELSGINFKDTLVE
                     FSKINRMSLVLLFIGGGASLVILSMYDVILSRALKMDISLGKVLRVSYIINALNAIVG
                     FGGFIGAGVRAMVYKNYTHDKKKLVHFISLILISMLTGLSLLSLLIVFHVFDASLILD
                     KITWVRWVLYVVSFFLPLFIIYSMVRPPDKNNRFVGLYCTLVSCVEWLAAAVVLYFCG
                     VIVDAHVSFMSFIAIFIIAALSGLVSFIPGGFGAFDLVVLLGFKTLGVPEEKVLLMLL
                     LYRFAYYFVPVIIALILSSFEFGTSAKKYIEGSKYFIPAKDVTSFLMSYQKDIIAKIP
                     SLSLAILVFFTSMIFFVNNLTIVYDALYDGNHLTYYILLAIHTSACLLLLLNVVGIYK
                     QSRRAIIFAMISILLITVATFFTYASYILITWLAIIFVLLIVAFRRARRLKRPVRMRN
                     IVAMLLFSLFILYVNHIFIAGTLYALDIYTIEMHTSVLRYYFWLTILIIAIIIGMIAW
                     LFDYQFSKVRISSKIEDCEEIINQYGGNYLSHLIYSGDKQFFTNENKTAFLMYRYKAS
                     SLVVLGDPLGDENAFDELLEAFYNYAEYLGYDVIFYQVTDQHMPLYHNFGNQFFKLGE
                     EAIIDLTQFSTSGKKRRGFRATLNKFDELNISFEIIEPPFSTEFINELQHVSDLWLDN
                     RQEMHFSVGEFNEEYLSKAPIGVMRNEENEVIAFCSLMPTYFNDAISVDLIRWLPELD
                     LPLMDGLYLHMLLWSKEQGYTKFNMGMATLSNVGQLHYSYLRERLAGRVFEHFNGLYR
                     FQGLRRYKSKYNPNWEPRFLVYRKDNSLWESLSKVMRVIRHK"
     gene            complement(5576..6085)
                     /gene="msrA"
                     /locus_tag="O2701_000633"
     CDS             complement(5576..6085)
                     /gene="msrA"
                     /locus_tag="O2701_000633"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCZ4292542.1"
                     /translation="MNINTAYFAGGCFWCMTKPFDTFDGIEKVTSGYMGGHIENPTYE
                     QVKSGTSGHLETVEIQYDVALFSYNKLLEIFFSVIDPLDTGGQYQDRGPQYQTAIFYT
                     NDHQKELAETYIEQLKNTINADKAIATKILPASQFYKAEDYHQDFYKKNPERYAEEQK
                     IRQEYKNKQ"
     gene            6222..7205
                     /locus_tag="O2701_000634"
     CDS             6222..7205
                     /locus_tag="O2701_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCZ4292543.1"
                     /translation="MDKETNDNEYRRQSEHRTSAPKRKKKKKIRKLPIILLIVVILLI
                     ALVVYIVHSYNSGVEYAKKHAKDVKVHQFNGPVKNDGKISILVLGADKAQGGQSRTDS
                     IMVVQYDFINKKMKMMSVMRDIYADIPGYGKHKINSAYALGGPELLRKTLDKNLGINP
                     EYYAVVDFTGFEKMIDELMPEGVPINVEKDMSKNIGVSLKKGNHRLNGKELLGYARFR
                     HDPEGDFGRVRRQQQVMQTLKKEMVNFRTVVKLPKVAGILRGYVNTNIPDSGIFQTGL
                     SFGIRGEKDVKSLTVPIKNSYEDVNTNTDGSALQINKNTNKQAIKDFLDED"
     gene            complement(7293..7478)
                     /locus_tag="O2701_000635"
     CDS             complement(7293..7478)
                     /locus_tag="O2701_000635"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HCZ4292544.1"
                     /translation="MPIVNVKLLEGRSDEQLKNLVSEVTDAVEKTTGANRQAIHVVIE
                     EMKPNHYGVAGVRKSDQ"
     gene            7626..8888
                     /locus_tag="O2701_000636"
     CDS             7626..8888
                     /locus_tag="O2701_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCZ4292545.1"
                     /translation="MYNYHLLEDRDVLCIDQKSFFASVSCIEKGLDPLETKLAVVADT
                     KRQGSVILAATPKLKELGIKTGSRLFEIPHRNDIYIINPSMRKYLNVSVAISKIALRY
                     IPPEDLHQYSIDEFFMDVTDSYHRFSSTVHAFCERLKREIYEETGIYCTVGIGSNMLL
                     SKIAMDVEAKHSQNGIAEWRYQDVPTKLWPIQPLRDFWGINRRTEAKLNKRGIFTIGD
                     LAKYPYKFLKKEFGILGVDMHLHANGIDQSKVREKHKISNPSICKSQILMRDYHFDEA
                     KVVMQELIEDVASRVRARKKVARTIHFAFGYSDEGGVHKQYTLKDPTNLEKDIYKVVM
                     HFADKLCNKQALYRTLSISLSQFINEDERQLSLFEDEYQRKRDECLAKTIDQLHLKYG
                     KGIVSKAVSFTEAGTKHGRLGLMAGHKM"
     gene            complement(9025..10116)
                     /locus_tag="O2701_000637"
     CDS             complement(9025..10116)
                     /locus_tag="O2701_000637"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HCZ4292546.1"
                     /translation="MTTVLFVGLGLIGGSLASNIKYHNPNTNIIAYDADTSQLDKAKS
                     IGIINEKCLNYSEAIKKADVIIYATPVAITNKYLSELIDMPTKPGVIVSDTGSTKAMI
                     QQHECNLLKHNIHLVSGHPMAGSHKSGVLNAKKHLFENAYYILVYNEPRNEQAANTLK
                     ELLSPTLAKFIVTTAEEHDYVTSVVSHLPHIVASSLVHVSQKNGQEHHLVNKLAAGGF
                     RDITRIASSNAQMWKDITLSNKTYILEMIRQLKSQFQDLERLIESNDSEKLLSFFAQA
                     KSYRDALPAKQLGGLNTAYDLYVDIPDESGMISKVTYILSLHNISISNLRILEVREDI
                     YGALKISFKNPTDRERGMQALSDFDCYIQ"
     gene            10281..11312
                     /locus_tag="O2701_000638"
     CDS             10281..11312
                     /locus_tag="O2701_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCZ4292547.1"
                     /translation="MEPILEMIKTLTGINSPSGDTEEAIQFVEKYAKDLGYQTTLTNK
                     GALLITVPGKNDEVQRCITAHVDTLGAMVKEIKEDGRLAIELIGGFTYNAIEGEYCQI
                     KTDAGQIYTGTICLHETSVHVYRNNHEIPRDQKHMEIRIDEVTTSEEDTKSLGISVGN
                     FVSFDPRTVITSSGFIKSRHLDDKASVAMILQLLKKLKEEQIILPHTTQFYISNNEEI
                     GYGANASIDSKIKEYIALDMGALGDGQASDEYTVSICAKDASGPYHKQLKSHLVNLCK
                     INNIPYKVDIYPYYGSDASAALHAGADIRHGLFGAGIESSHAMERTHIDSIKATEKLL
                     YAYCLSPIE"
     gene            11804..13210
                     /locus_tag="O2701_000639"
     CDS             11804..13210
                     /locus_tag="O2701_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCZ4292548.1"
                     /translation="MDIFYKKIKANVTPEVLAQLHSKKIILESTNQQQTKGRYSVVIF
                     DIYGTLTLDNDVLSVSTLKESYQITERPYHYLTTKINEDYHNIQDEQLKSLPFISGYV
                     GTCSFDLVRHEFPKLQSIQLEDHKQHDVRLYMVEQVYVFDHYKDELYIIATNQFSNST
                     KSDLENRVIKSIEDLTKIQPFMPTQDFDFKTKEIQSNISEERFIEMIQYFKEKITEGD
                     MFQVVPSRIYKYAHHASQHLNQLSFQLYQNLKRQNPSPYMYYLNIDQPYIVGSSPESF
                     VSVKDQIVTTNPIAGTIQRGETTQIDNENMKQLLNDPKECSEHRMLVDLGRNDIHRVS
                     KIGTSKITELMVIEKYEHVMHIVSEVTGKINQNLSPMTVIANLLPTGTVSGAPKLRAI
                     ERIYEQYPHKRGVYSGGVGYINCNHNLDFALAIRTMMIDEQYINVEAGCGVVYDSIPE
                     KELNETKLKAKSLLEVSP"
     gene            13207..13773
                     /locus_tag="O2701_000640"
     CDS             13207..13773
                     /locus_tag="O2701_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HCZ4292549.1"
                     /translation="MILVVDNYDSFTYNLVDIVAQHTDVIVQYPDDDNVLNQSVDAVI
                     ISPGPGHPLDDQQLMKIISTYQHKPILGICLGAQALTCYYGGEVIKGDKVMHGKVDTL
                     KVISHHQHLLYQDIPEQFSIMRYHSLISNPDNFPEELKITGRTKDCIQSFEHKERPHY
                     GIQYHPESFATDYGVKIITNFINLVKEG"
     gene            13779..14777
                     /gene="trpD"
                     /locus_tag="O2701_000641"
     CDS             13779..14777
                     /gene="trpD"
                     /locus_tag="O2701_000641"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HCZ4292550.1"
                     /translation="MTLLTRIKTETILLESDIKELIDILISPSIGTDIKYELLSSYSE
                     REIQQQELTYIVRSLINTMYPHQPCYEGAMCVCGTGGDKSNSFNISTTVAFVVASAGV
                     KVIKHGNKSITSNSGSTDLLNQMNIQTTTVDDTPNQLNEKDLVFIGATESYPIMKYMQ
                     PVRKMIGKPTILNLVGPLINPYHLTYQMVGVFDPTKLKLVAKTIKDLGRKRAIVLHGA
                     NGMDEATLSGDNLIYELTEDGEIKNYTLNATDYGLKHAPNSDFKGGSPEENLAISLNI
                     LNGKDQSSRRDVVLLNAGLSLYVAEKVDTIAEGIELATTLIDNGEALEKYHQMRGE"
     gene            14779..15561
                     /gene="trpC"
                     /locus_tag="O2701_000642"
     CDS             14779..15561
                     /gene="trpC"
                     /locus_tag="O2701_000642"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HCZ4292551.1"
                     /translation="MTILSEIVKYKQSLLQNGYYQDKLNTLKSVKIQNKKSFINAIEK
                     EPKLAIIAEIKSKSPTVNDLPERDLSQQISDYDQYGANAVSILTDEKYFGGSFERLQA
                     LTTKTTLPVLCKDFIIDPLQIDVAKQAGASMILLIVNILSDKQLKDLYNYAISQNLEV
                     LVEVHDRHELERAYKVNAKLIGVNNRDLKRFVTNVEHTNTILENKKTNHYYISESGIH
                     DASDVRKILHSGIDGLLIGEALMRCDNLSEFLPQLKMQKVKS"
     gene            15561..16193
                     /locus_tag="O2701_000643"
     CDS             15561..16193
                     /locus_tag="O2701_000643"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HCZ4292552.1"
                     /translation="MKLKFCGFTSIKDVTAASQLPIDAIGFIHYEKSKRHQTITQIKK
                     LASAVPNHIDKVCVMVNPDLTTIEHVLSNTSINTIQLHGTESIDFIQEIKKKYSSIKI
                     TKALAADENIIQNINKYKGFVDLFIIDTPSVSYGGTGQTYDWTILKHIKDIPYLIAGG
                     INSENIQTVNQLKLSHQGYDLASGIEVNGRKDIEKMTAIVNIVKGDRDNE"
     gene            16186..17400
                     /gene="trpB"
                     /locus_tag="O2701_000644"
     CDS             16186..17400
                     /gene="trpB"
                     /locus_tag="O2701_000644"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCZ4292553.1"
                     /translation="MNKQIQTEADELGFFGEYGGQYVPETLMPAIIELKKAYKEAKAD
                     PEFQRELEYYLSEYVGRATPLTYAASYTESLGGAKIYLKREDLNHTGAHKINNALGQA
                     LLAKRMGKKKLVAETGAGQHGVASATVAALFDMELVVFMGSEDIKRQQLNVFRMELLG
                     AKVVAVEDGQGTLSDAVNKALQYWVSHVDDTHYLLGSALGPDPFPTIVRDFQSVIGKE
                     IKSQILKKEGRLPDAIVACIGGGSNAIGTFYPFIKDDVALYGVEAAGQGDDTDKHALA
                     IGKGSPGVLHGTKMYLIQDEDGQVQLAHSISAGLDYPGIGPEHSYYHDIGRVTFENAS
                     DTQAMNALINFTKHEGIIPAIESAHALSYVERLAPTMSKEDIIVVTISGRGDKDMETI
                     RQYMVERGLAND"
     gene            17393..18121
                     /locus_tag="O2701_000645"
     CDS             17393..18121
                     /locus_tag="O2701_000645"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCZ4292554.1"
                     /translation="MTKLFIPYIMGNKDLIENATLLSENGADIIEIGVPFSDPVADGP
                     VIMEAGQQAIKQGITIDYIFNQLEKHGDQIKCNYVLMTYYNIICHYGEQAFFEKCRDT
                     GVYGLIIPDLPYELSQRLKQQFSHYGVKIISLVAMTTDDKRIKDIVSHAEGFIYTVTM
                     NATTGQNGAFHPELKRKIESIKAIANVPVVAGFGIRTPQHVADIKEVADGIVIGSEIV
                     KRFKSNTREEIIKYLQSIQQTLNN"
     gene            18443..19705
                     /gene="femA"
                     /locus_tag="O2701_000646"
     CDS             18443..19705
                     /gene="femA"
                     /locus_tag="O2701_000646"
                     /EC_number="2.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemA"
                     /protein_id="HCZ4292555.1"
                     /translation="MKFTNLTAKEFGAFTDSMPYSHFTQTVGHYELKLAEGYETHLVG
                     IKNNNNEVIAACLLTAVPVMKVFKYFYSNRGPVIDYENQELVHFFFNELSKYVKKHRC
                     LYLHIDPYLPYQYLNHDGEITGNAGNDWFFDKMSNLGFEHTGFHKGFDPVLQIRYHSV
                     LDLKDKTADDIIKNMDGLRKRNTKKVKKNGVKVRFLSEEELPIFRSFMEDTSESKAFA
                     DRDDKFYYNRLKYYKDRVLVPLAYINFDEYIKELNEERDILNKDLNKALKDIEKRPEN
                     KKAHNKRDNLQQQLDANEQKIEEGKRLQEEHGNELPISAGFFFINPFEVVYYAGGTSN
                     AFRHFAGSYAVQWEMINYALNHGIDRYNFYGVSGKFTEDAEDAGVVKFKKGYNAEIIE
                     YVGDFIKPINKPVYAAYTALKKVKDRIF"
     gene            19724..20983
                     /gene="femB"
                     /locus_tag="O2701_000647"
     CDS             19724..20983
                     /gene="femB"
                     /locus_tag="O2701_000647"
                     /EC_number="2.3.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemB"
                     /protein_id="HCZ4292556.1"
                     /translation="MKFTELTVTEFDNFVQNPSLESHYFQVKENIVTRENDGFEVVLL
                     GIKDDNNKVIAASLFSKIPTMGSYVYYSNRGPVMDFSDLGLVDYYLKELDKYLQQHQC
                     LYVKLDPYWLYHLYDKDIVPFEGREKNDALVNLFKSHGYEHHGFTTEYDTSSQVRWMG
                     VLNLEGKTPETLKKTFDSQRKRNINKAINYGVKVRFLERDEFNLFLDLYRETEERAGF
                     VSKTDDYFYNFIDTYGDKVLVPLAYIDLDEYVLKLQQELNDKENRRDQMMAKENKSDK
                     QMKKIAELDKQIDHDQHELLNASELSKTDGPILNLASGVYFANAYEVNYFSGGSSEKY
                     NQFMGPYMMHWFMINYCFDNGYDRYNFYGLSGDFTENSEDYGVYRFKRGFNVQIEELI
                     GDFYKPIHKVKYWLFTTLDKLRKKLKK"
     gene            21672..22439
                     /locus_tag="O2701_000648"
     CDS             21672..22439
                     /locus_tag="O2701_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCZ4292557.1"
                     /translation="MRFVFDIDGTLCFDGRLIDQTIIDTLLQLQHDGHELIFASARPI
                     RDLLPVLPSVFHQHTLIGANGAMISQQSKISVIKPIHTDTYHHIFKIIQKYELDYIID
                     DDWNYAAQLDAENAIFERLDPHKLASCIDVANIDTPIKIILLNIDPAQITTILDELDK
                     YHQELEMIHHSNEYNIDITAQNINKYTALQYIFDADVKYIAFGNDHNDIVMLQHASSG
                     YIIGPSEAYTHAILKLDKIKHINNNAQAICKVLKSYK"
     gene            complement(22487..22960)
                     /locus_tag="O2701_000649"
     CDS             complement(22487..22960)
                     /locus_tag="O2701_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HCZ4292558.1"
                     /translation="MGLLDIASIRSIERGFNYYQSECVINLKSFSETQHEAEVKGSGN
                     KVYRCYIDMEHPRKSICNCPHADGRRVICKHMIALLFTASPEAANKHIMMLNEVEEDY
                     QLRRNMWIDSLKEMINDMSEEELRDAYLNMLIEHGEMAELFGLDEEEEMFEDEFY"
     gene            complement(23084..23785)
                     /locus_tag="O2701_000650"
     CDS             complement(23084..23785)
                     /locus_tag="O2701_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4292559.1"
                     /translation="MIELKHVTFGYNKKQMVLQDINITIPDGENVGILGESGCGKSTL
                     ASLVLGLFKPVKGEIYLSDNAVLPIFQHPLTSFNPDWTIETSLKEALYYYRGLTDNTA
                     QDQLLLQHLSTFELNAQLLTKLPSEVSGGQLQRFNVMRSLLAQPRVLICDEITSNLDV
                     IAEQNVINILKAQTITNLNHFIVISHDLSVLQRLVNRIIVLKDGMIVDDFAIEELFNV
                     DRHPYTKELVQAFSY"
     gene            complement(23778..24551)
                     /locus_tag="O2701_000651"
     CDS             complement(23778..24551)
                     /locus_tag="O2701_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4292560.1"
                     /translation="MSLIDIQNLTIKNTSEKSLIKGIDLKIFSQQINALIGESGAGKS
                     LIAKALLEYLPFDLSCTYDSYQFDGENVSRLSQYYGHTIGYISQNYAESFNDHTKLGK
                     QLTAIYRKHYKGSKEEALSKVDKALSWVNLQSKDILNKYSFQLSGGQLERVYIASVLM
                     LEPKLIIADEPVASLDALNGNQVMDLLQHIVLEHGQTLFIITHNLSHVLKYCQYIYVL
                     KEGQIIERGNINHFKYEHLHPYTERLIKYRTQLKRDYYD"
     gene            complement(24538..25368)
                     /locus_tag="O2701_000652"
     CDS             complement(24538..25368)
                     /locus_tag="O2701_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4292561.1"
                     /translation="MHKIFSKNNLIFFVFVAFIFVVIVLQFFVSSENATKVNLSQTFE
                     PISWLHLLGTDDYGRDLFTRIIIGARSTLFVTVLTLIAIVVIGVTLGLFAGYKKGWIE
                     RLVLRFIDVGLSIPEFIIMIALASFFQPSLWNLVISITLIKWMNYTRLTRSIVNSEMN
                     KPYIKMAQLFHVPTRTILIRHLTPKIIPAIIVLMVVDFGKIILYISSLSFIGLGAQPP
                     TPEWGAMLQQGRDFISSHPIMLIAPASVIAITILIFNLTGDALRDRLLKQRGEYDESH
                     "
     gene            complement(25361..26347)
                     /locus_tag="O2701_000653"
     CDS             complement(25361..26347)
                     /locus_tag="O2701_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4292562.1"
                     /translation="MFIIKSMLYRLMQMIVVLFVISTLTFILMKLSPGNPVDKILHLD
                     VAQVSTEQINATKDKLGLNDSLLVQWWHWMNHLLHFNLGKSFESKEPVTQILFNYAPI
                     TLLISFSTLVVSLCISIPLGIIAAKRFHKWTDKVIRVISTLSISLPAFFIGIILLFIV
                     TNLMNIDSVILSQFILPVITLSLGMCAYIIRLVRSNLLMLLQSNIVQASRLRGMNERY
                     ILIHDLLKPTILPIIPLLGISLGSLIGGTVVIENLFDIPGIGYLLMDSIKSRDYPVIQ
                     GCVLFIGFFVVIINTIADLLTLLLDPKQRLQLGNPKIKTNTPLISESSDRHA"
     gene            complement(26518..26703)
                     /locus_tag="O2701_000654"
     CDS             complement(26518..26703)
                     /locus_tag="O2701_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292563.1"
                     /translation="MKNTAMRALNFAVKIKNNIRQLYAQNHRLLSYTAVTIISKEMEY
                     SAIVVFVLTFLYFTWLT"
     gene            complement(26651..26995)
                     /locus_tag="O2701_000655"
     CDS             complement(26651..26995)
                     /locus_tag="O2701_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292564.1"
                     /translation="MTLFDMPNYLWITTLIMILLTIFCCLVLNKWFVSAVITFVILGV
                     LAFFIPNFQDIKYQPLLGYAAFLAIMSLLISFLLWYFTRNWRKERKARKLEKQIEKYG
                     YEGAELRRKDKK"
     gene            27200..29014
                     /gene="pepF"
                     /locus_tag="O2701_000656"
     CDS             27200..29014
                     /gene="pepF"
                     /locus_tag="O2701_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCZ4292565.1"
                     /translation="MSQGLPLREDVPVSETWDLVDLFKDDQQYYESIDALVQQANQFH
                     HTYATTLNSIEQINTALAELENILIALDRLSNYAELRLSVDTSNIEAQVLSAKLSTTY
                     GKIVSQLSFVESEILELPEEILQQLEESCPYQHYIKQLIKQKPFQLSASVEQVLATLS
                     PTLNSPYDLYGTTKMLDITFDSFEHDGTTYPVDYATFENDYEDNKDPEFRRKSFKSFS
                     DGIRKYQHTTAATYNMQVQQEKIEADLRGFESVIDYLLHSQEVTRDMFDRQIDMIMRD
                     LAPVMQKYAKLLQRIHGLDNMRFEDLKISVDPDYEPEISIEDSKNYIFGALSVLGDDY
                     TNMLREAYDQRWIDFAQNKGKDTGAFCASPYFTHSYVFISWTGKMAEAFVLAHELGHA
                     GHFTLAQKHQPYLESEASMYFVEAPSTMNEMLMANYLFNTSDNPRFKRWVIGSILSRT
                     YYHNMVTHLLEAAYQREVYHKVDQGESLNAPTLNEIMLNVYKQFFGDAVDMTEGAELT
                     WMRQPHYYMGLYSYTYSAGLTIGTVVSQKIKNEGQPAVDAWLETLKKGGSVSPVELAN
                     IAGVDITTEQPLKSTIQYISDLVDEVEKLTDEIEQANN"
     gene            complement(29154..29795)
                     /gene="phoU"
                     /locus_tag="O2701_000657"
     CDS             complement(29154..29795)
                     /gene="phoU"
                     /locus_tag="O2701_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCZ4292566.1"
                     /translation="MAIIRQRYQEQLDDLIKELRRLGANVYVSIENGIKSLSIDDRGF
                     ARQTVKNDKHINQLNYDINERVIMLITKQQPIASDLRMMISSLKIASDLERIGDNASS
                     IANIRLRTKITDDYVLTRLKTMGKLAMLMLKDLDQAFKKKDTVLIREIIERDEDIDDL
                     YSHIINATYLIDNDPFVAAQAHLAARHLERIGDHIINIAESVYFYLTGTHYEQ"
     gene            complement(29802..30653)
                     /locus_tag="O2701_000658"
     CDS             complement(29802..30653)
                     /locus_tag="O2701_000658"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCZ4292567.1"
                     /translation="MAQTLAQTKQISQSHTFDVSQSHHKTPDDTNSHSVIYSTQNLDL
                     WYGENHALQNINLDIYENQITAIIGPSGCGKSTYIKTLNRMVELVPSVKTAGKILYRD
                     QDIFDQKYSKEQLRTNVGMVFQQPNPFPKSIYDNITYGPKIHGIKNKKVLDEIVEKSL
                     RGAAIWDELKDRLHTNAYSLSGGQQQRVCIARCLAIEPEVILMDEPTSALDPISTLRV
                     EELVQELKEKYTIIMVTHNMQQAARVSDKTAFFLNGYVNEYDDTDKIFSNPSNKKTED
                     YISGRFG"
     gene            complement(30700..31617)
                     /gene="pstA"
                     /locus_tag="O2701_000659"
     CDS             complement(30700..31617)
                     /gene="pstA"
                     /locus_tag="O2701_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCZ4292568.1"
                     /translation="METTDNNRQSLVDQQLVQKHLSSRTVKNKVFKLIFLACTLLGLV
                     VLIALLTQTLIKGVSHLNLQFFTNFSSSTPSMAGVKGALIGSLWLMLSIIPLSIILGI
                     GTAIYLEEYAKNNKFTQFVKISISNLAGVPSVVFGLLGYTLFVGGAGIEALKMGNSIL
                     AAALTMTLLILPIIIVSSQEAIRAVPNSVREASYGLGANKWQTIRRVVLPAALPGILT
                     GFILSLSRALGETAPLVLIGIPTILLATPRSILDQFSALPIQIFTWAKMPQEEFQNVA
                     SAGIIVLLVILILMNGVAIILRNKFSKKF"
     gene            complement(31619..32545)
                     /gene="pstC"
                     /locus_tag="O2701_000660"
     CDS             complement(31619..32545)
                     /gene="pstC"
                     /locus_tag="O2701_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HCZ4292569.1"
                     /translation="MTSSTNVKALIEKNNNKKGKHNDKIIPVILAAISAISILTTLGI
                     LITLLLETITFFTRIPITEFLFSTTWNPTGSDPKFGIWALIIGTLKITVIATIFAVPV
                     GLGAAIYLSEYASDRARRIIKPILEILAGIPTIVFGFFALTFVTPVLRSFIPGLGEFN
                     AISPGLVVGIMIVPLITSLSEDAMASVPNKIREGAYGLGATKLEVATKVVLPAATSGI
                     VASIVLAISRAIGETMIVSLAAGSSPTASLSLTSSIQTMTGYIVEIATGDATFGSNIY
                     YSIYAVGFTLFIFTLIMNLLSQWISKRFREEY"
     gene            complement(32736..33719)
                     /locus_tag="O2701_000661"
     CDS             complement(32736..33719)
                     /locus_tag="O2701_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCZ4292570.1"
                     /translation="MKKWQFVGTTALGATLLLGACGGGNGGSGNSDLKGEAKGDGSST
                     VAPIVEKLNEKWAQDHSDAKISAGQAGTGAGFQKFIAGDIDFADASRPIKDEEKQKLQ
                     DKNIKYKEFKIAQDGVTVAVNKENDFVDELDKQQLKAIYSGKAKTWKDVNSKWPDKKI
                     NAVSPNSSHGTYDFFENEVMNKEDIKAEKNADTNAIVSSVTKNKEGIGYFGYNFYVQN
                     KDKLKEVKIKDENGKATEPTKKTIQDNSYALSRPLFIYVNEKALKDNKVMSEFIKFVL
                     EDKGKAAEEAGYVAAPEKTYKSQLDDLKAFIDKNQKSDDKKSDDKKSEDKK"
BASE COUNT        11801 a         5124 c         5692 g        11324 t
ORIGIN      
        1 atatgggagt cagatttttt tattatagtt attccacttt gagagacatc gaaattaaat
       61 taagatatat catataggat aggcgtgtaa tagaacacaa gtatttaaat tattatactt
      121 tcaagttaga catttccgat atataatctt aataatagaa aaataggtga tgataatggg
      181 agaatatatt gttactaaaa cattgaacaa caatgtcgta gtatgtacta ataatgatca
      241 agaagttatt ttaatcggta aaggtattgg ttttaacaaa aaagagggaa tggcgttaaa
      301 cgaccaaact attacaatag agaaaattta taaattagaa agtgagcaac aaaaagcaca
      361 ttataaaagt ttagttgaaa tcgctgatga taatgtatta caagtaatta ttgattcgtt
      421 gaattttatt tctaatactg cgatgaatgt tgattcaaaa caacttgtag tttcattaac
      481 ggatcatatt atatttgctt ataaacgctt aaaacaaaat caagttatta gcaatccatt
      541 tgttatggaa actatgcagt tatatagtga tgcatatcat attgctaaac aggtgattga
      601 tcagttaaat gcagcattag atgtacattt tcctgaagat gagataggat ttattgcatt
      661 acatattgca tctaatacag aagatttatc tatgcatgag atgaccttga tcaataatgt
      721 tattaaaaaa ggtatagata tcattgaatc agaccttgtg acaactgttg ataaggaatc
      781 attacaatac caacgtttta taaggcacgt acaattttta attcgccgat taagaagaaa
      841 agaatatata catgcacaag atgattttgt gtctatgatt aaaaatcact atccgatttg
      901 ctataacaca gcatataaaa ttttaactat gatacaaaaa caatttgatg ttaatatcag
      961 tgagtctgaa attatatatt taacattaca cattcatcat tttgaagaaa ggattaatca
     1021 atcctaaatt aagtgtatta cacgggcgtt tcgttactgt aaagggtatt tgtatggtat
     1081 cattgatgtt agtaatttta cataagtgga ctgtttttat acaacatctt atgaatattt
     1141 taagtggtgg tgaatctata attgaatgaa aatgaaaaga atataagaaa gaatttttta
     1201 aaatttaccg aatcacggta tatgaagttt gttggtggga atgatttagt cttctcatta
     1261 atagcgctag tattgttggg tattgttatt tttattttcg aaaaagtatc atatgttttt
     1321 gatcctttta tcatcgtttt taagacgata gcagcaccta tcatcgtctc tttaattcta
     1381 ttctatctat ttaacccaat cgtaaatatg atggaacgtt atagaatacc aagagttgca
     1441 ggtatttcta ttatttatct agctgtagta ggtgttatta cgttaattgt taatttattg
     1501 atacctatta ttggttcgca agtagatagt ttagttaaaa attcaccgca atatctagaa
     1561 aaattaatta attctattga taaaatagca aataatacgt ttttctcttc gtattatagt
     1621 caaattaatg attggttaaa ttctttacct aagaaaatac catctatgtt aagtgaattt
     1681 acagatggct ttgggtctaa aattgcaacg tttgcagaaa cgattgctaa tattggcgtt
     1741 gtgattgtca caacaccatt tgtactattc tttatgctta aagatggaca tcacttcaaa
     1801 gaattttcaa cgaatattat gccaccgaaa ttccgaaaag attttcatga tctacttgaa
     1861 aaaatgagtg ttcaagttgg ttcatacatt caaggacaaa ttatcgtttc attctgtatc
     1921 ggtatactgt tgtttatcgg ttattcggtt atcgggttga aatatagctt agtattagct
     1981 agtattgcgg cagttacaag tgttgtacca tatttagggc ctactatagc gatttctcca
     2041 gctattgtaa tagctgctat aacatcgccg tggatgctct taaaattagc agtagtatgg
     2101 actttagtac aatttgttga agggcacttc atttcaccaa atatcatggg taaaacactt
     2161 aagattcatc cacttacaat cattttcatt ttactgtgtg caggcaaatt gcttggtatt
     2221 gtaggcgtta ttttaggtat tccgggatat gctattttaa aagtattagt tactcattta
     2281 ttccaattat ttaaacgtcg atacaatcgt ttctatggta atgatgtagg tgaatatgat
     2341 attaaagaaa gtaataaaat agttgaataa gtaataaaaa ataccaatga ctatatgttt
     2401 gttggtattt tttatggtgc ttataatgtt gggcagttac atttatgatt atcagcacat
     2461 gtttagctta taaattttta cgaaagttta gccgtttata aagcacatgc ataatgaaac
     2521 gagtatttgc cacttgatta gtacttcatt attatgtcga aaataaaaat aagtggtatt
     2581 tttaatatat taagaagcac tcataatcgg ctgttaatta ataatatttt tcataagtat
     2641 tgattcatca ttttctttat gttaaatatg aatcagtata gataaccata ttgttctgtt
     2701 tgagatagaa atattttcta tttacttctt aatacagata tcaatatgac aaaagtgtta
     2761 tataaatcaa aggtaaatga tatgctattt ttatggttta tatgatatat tttgatttat
     2821 aacagaaata attagaattg atgtgaaaaa atgaatcagg aagttaaaaa caaaatattt
     2881 tcaatcttaa aaattacgtt tgctacagct ttatttattt ttgtagcaat cacattgtat
     2941 cgggagttat ctggtattaa ctttaaagat acgttggttg aatttagtaa gattaaccgt
     3001 atgtccttag tgttactatt tattggtggt ggggcatcgc ttgttattct atcaatgtat
     3061 gatgtgattt tatctagagc tttaaaaatg gatatatcct taggcaaagt tttaagagta
     3121 agttatatca tcaatgcatt gaatgcgatt gtaggtttcg gtggctttat tggtgcaggc
     3181 gttagagcaa tggtttataa aaactatacg catgataaaa agaaattagt tcactttata
     3241 tccttaatac ttatttcaat gttgacaggt ttaagcttat tatcattgct aattgtattc
     3301 catgttttcg atgcatcttt aatcttagat aagattacat gggtaagatg ggtattatat
     3361 gtagtgtcat ttttcttacc attattcatt atttattcaa tggttagacc acccgataaa
     3421 aacaatcgtt ttgtaggatt gtactgcact ttagtgtcgt gtgttgaatg gttagcagct
     3481 gcagttgtat tatatttctg tggtgtaatt gttgacgctc atgtatcatt catgtccttt
     3541 attgcaatat ttatcattgc tgcattatca ggtttagtca gctttattcc tggtggtttc
     3601 ggcgctttcg atttagttgt attactagga tttaaaactt taggtgtccc tgaggaaaaa
     3661 gtattattaa tgctacttct atatcgtttt gcgtactatt ttgtaccggt aattattgca
     3721 ttaattttat catcatttga atttggtaca tcagctaaga agtacattga gggatctaaa
     3781 tactttattc ctgctaaaga tgttacgtca tttttaatgt cttatcaaaa ggatattatt
     3841 gctaaaattc catcattatc attagcaatt ttagtattct ttacaagtat gatctttttt
     3901 gtaaataact taacgattgt ttacgatgct ttatatgatg gaaatcactt aacgtattat
     3961 attctattgg caattcatac tagtgcttgt ttattacttt tactgaatgt agttggtatt
     4021 tataagcaaa gtagacgtgc cattatcttt gctatgattt caattttatt aatcacagtg
     4081 gcgacattct tcacttacgc ttcatatatt ttaataacat ggttagctat tatttttgtt
     4141 ctgcttattg tagctttccg tagagcacgt aggttgaaac gcccagtaag aatgagaaat
     4201 atagttgcaa tgcttttatt cagtttattt attttatatg ttaaccatat atttattgct
     4261 ggaacgttat atgcattaga tatttatacg attgaaatgc atacatctgt attgcgctat
     4321 tacttctggc ttacgatttt aatcatcgct atcatcatag gtatgattgc atggttgttt
     4381 gattatcaat ttagcaaagt acgtatttct tctaaaattg aagattgcga ggagattatt
     4441 aatcagtacg gcggtaatta tttgagtcac ttgatatata gtggtgacaa gcagtttttc
     4501 actaatgaaa ataaaacagc atttttaatg tatcgttata aagcaagttc attagtggtt
     4561 cttggagatc cgttaggtga tgaaaatgcc tttgatgaat tgttagaagc attctataat
     4621 tacgctgagt atttaggcta tgatgttata ttctatcaag ttacagatca acacatgcct
     4681 ttatatcata atttcggtaa ccaatttttc aaattaggtg aagaagcaat tattgattta
     4741 acgcaatttt caacttcagg taaaaaacgc cgtggattta gagcgacttt aaataaattc
     4801 gatgaactta atatttcgtt cgaaattatt gaaccaccgt tttcaactga atttataaat
     4861 gaacttcaac atgtaagtga tttatggcta gataatcgtc aggaaatgca tttctctgtt
     4921 ggtgaattta atgaagaata cttatctaaa gcgccaattg gtgtaatgcg aaatgaagaa
     4981 aatgaagtaa ttgcattttg tagtttaatg ccaacatact ttaatgatgc catttcagtc
     5041 gatttaatta gatggttgcc agagttagat ttaccattaa tggatggtct atacttgcat
     5101 atgttacttt ggagtaaaga acaaggttat acaaaattta atatgggtat ggcaacgtta
     5161 tcgaacgttg gtcaattgca ttattcatat ttaagagaac gacttgcagg ccgtgtcttt
     5221 gaacatttca acggtctata tcgtttccaa ggattacgtc gttataaatc taaatataat
     5281 ccgaattggg aaccacgctt tttagtttat cgtaaagata attcgctttg ggaatcactt
     5341 tctaaagtaa tgcgtgtaat acgtcacaaa taattaaaat ccaagtgcta agaggtatac
     5401 agttatgcct gatgcgcttg gattttttgg tttctataaa gaatgattta taaaaaacgg
     5461 tacagataag aatatttaag tttcctatct gtaccgtatt ttaatattga aatgagctaa
     5521 agttaagtcg cctaactttt attatcagtc caatcagttt catcaagtat aaatgttatt
     5581 gcttattttt gtattcttgg cgtatttttt gttcttctgc atagcgctct ggatttttct
     5641 tataaaaatc ttggtgatag tcttcggctt tgtaaaattg tgacgctggt aatatttttg
     5701 ttgcaattgc cttatcagca ttaatcgtat ttttaagctg ctcgatataa gtctcagcga
     5761 gttctttttg atgatcatta gtgtagaaaa tagctgtttg atattgagga ccacggtctt
     5821 gatattgacc acctgtatct aatgggtcaa tgactgagaa aaatatttct aataacttat
     5881 tgtatgagaa taatgcaaca tcatattgaa tttcaacagt ttctaaatga ccactcgtac
     5941 ctgattttac ttgttcgtaa gtaggatttt caatatgtcc gcccatatat ccagaagtta
     6001 ctttttctat gccgtcaaag gtgtcaaatg gtttcgtcat acaccaaaag caacctccgg
     6061 caaaataagc tgtattaata ttcatttttg acatcctttc attagacctt agtacgattt
     6121 attaagaaat cacttgcttt ttgaattgtt tttatataac gttaatatgt gattattata
     6181 gtacacaaaa atctattttt gaagatataa ggtaggtaaa tatggataaa gaaactaatg
     6241 acaacgaata tagacgtcaa agtgaacatc gcacttcggc gcctaagcga aaaaagaaga
     6301 agaaaattag gaaattacct atcattcttc tgattgttgt aattttactt atcgcattag
     6361 ttgtatatat tgtacatagt tacaatagcg gtgtagaata tgccaagaaa catgcgaaag
     6421 atgttaaagt acatcaattt aatggaccag taaaaaatga tggtaaaatt tctattcttg
     6481 tactcggtgc agataaagca caaggtggac aatcaagaac agattctatc atggttgttc
     6541 aatatgactt tatcaataaa aagatgaaaa tgatgtctgt catgcgtgat atttatgcag
     6601 atattccagg atatggaaaa cacaaaatta attcagcata cgctttaggt ggtccagagc
     6661 tacttagaaa aacacttgat aaaaatttag gaattaatcc tgaatattat gcagtagttg
     6721 attttactgg atttgagaaa atgattgatg aattaatgcc tgaaggtgta ccaattaatg
     6781 tcgaaaaaga tatgtcgaaa aatattggtg tatctttgaa aaagggtaac cataggttga
     6841 atggtaaaga attacttggt tatgcaagat tccgtcacga ccctgaaggt gacttcggac
     6901 gcgtgcgacg tcagcaacaa gtgatgcaaa cattgaaaaa agaaatggtt aattttagaa
     6961 cagttgttaa attaccaaaa gttgcaggta ttttaagagg ctatgtgaat acaaacattc
     7021 ctgattcagg gattttccaa acaggtttga gttttggtat ccgaggtgaa aaagatgtta
     7081 agtcattgac tgtgccaatc aagaactcat acgaagatgt caatacaaat actgatggta
     7141 gtgcattaca gattaataaa aacacaaata aacaagctat taaagacttt ttagatgaag
     7201 attaaaaata aacaaggcga tttctatcat actcagatag aagtcgcctt gttcgttttc
     7261 aaagaattaa ttggcatgca tcttatgaag aattattgat ctgactttct tacgccagcc
     7321 acaccataat ggtttggttt catttcttct ataacaacgt gaattgcttg tctatttgcc
     7381 cccgttgttt tttctacggc gtcagttact tcgctaacta aattttttaa ttgttcatcc
     7441 gaacgacctt ctaataattt tacattgacg attggcatca tccaaacctc ctaatcatta
     7501 ttaatttgag tatatcatgt aatacttaaa aattcatttg caaaataaca gaacatttgt
     7561 tctaaaatag ttgaaataga acacgtgttc gtatataata agattcaagt aagggaggaa
     7621 taacagtgta taattatcat ttattagaag atagggatgt tctatgtatt gaccaaaaaa
     7681 gtttttttgc gagtgtttct tgtattgaaa aggggctaga tccattagaa acaaagctag
     7741 ctgttgttgc agatactaag cgtcagggtt ctgtaatatt ggctgcgaca cctaaattaa
     7801 aagaattagg catcaagaca gggtcgcgat tgtttgaaat accacataga aatgatattt
     7861 acattatcaa tccaagtatg cgtaaatatc ttaatgtttc agttgctatt tctaagattg
     7921 cattgcgtta tattccacct gaagatttac accaatatag tattgacgaa ttttttatgg
     7981 atgttactga tagctatcat agatttagtt ctacagtaca tgcattttgc gaaagactta
     8041 aacgtgaaat ttatgaagaa acaggcattt attgtactgt gggcattggt tctaatatgt
     8101 tattaagtaa aattgctatg gatgttgaag cgaagcatag tcaaaatggt atagctgaat
     8161 ggcgatatca agatgtacca acgaaattat ggccaattca gcccttgcga gatttttggg
     8221 gtattaatcg tcgaacagaa gccaaattga ataaaagagg aatttttact ataggagatt
     8281 tagcgaaata tccatataaa tttttaaaaa aagagttcgg tattttaggt gttgatatgc
     8341 atctacatgc gaatgggata gatcagagta aagtacgtga aaagcacaag atcagcaatc
     8401 catcgatatg caaaagtcaa atattaatga gagattatca ttttgatgaa gcaaaagtag
     8461 taatgcaaga gttaattgaa gatgttgcta gcagagttcg agcaagaaaa aaagtggcaa
     8521 gaacgataca ttttgccttt ggctatagtg atgaaggcgg tgtacataag caatatactt
     8581 tgaaagatcc aacaaactta gaaaaagata tttataaagt agtaatgcat ttcgcagata
     8641 aattatgtaa taaacaagca ctatatcgta cgctaagtat atctttgagt caatttatta
     8701 atgaggatga gcgacagtta agtctgtttg aagatgaata ccaacgcaaa cgtgacgaat
     8761 gtctagctaa aacgatagac caattacatt tgaaatacgg caaaggtatt gtgtccaaag
     8821 cagtatcgtt tacagaagca ggtacaaaac acggcagatt aggtttaatg gctggacata
     8881 aaatgtaatg actatacggt ttaagtaata tataactgtg attcgtataa aataagtctc
     8941 taaagataaa tatttcatat atcacaatag attttcacaa taatatctaa gaatacatgg
     9001 aatttatcaa aagagactta ataattattg gatataacaa tcaaaatcac tcaatgcttg
     9061 cataccgcgt tctcggtcag tagggttttt gaaactaatt tttaaagcac cgtatatatc
     9121 ttcgcgtact tctaagattc ttaagttgct tatagatatg ttatgtaaac tcaggatata
     9181 agtcacttta cttatcatac ctgattcatc cggaatgtct acatatagat catacgcagt
     9241 atttagtcca cctagttgtt tagcgggtag tgcgtcgcga tacgatttag cttgggcaaa
     9301 aaatgataac aatttttcag aatcattgct ttcaattagt ctttctaaat cttgaaactg
     9361 actttttagc tgtcgaatca tttctaaaat atacgtttta ttactcaagg tgatatcttt
     9421 ccacatttgt gcattactac tagctatacg agtgatatca cgaaaaccac cagctgcaag
     9481 tttattaact aaatgatgtt cttgaccgtt cttttgacta acatgaacta aactagatgc
     9541 aacgatatga ggtaaatgac ttacgacgct tgttacgtag tcgtgttctt cagcagtagt
     9601 tacaataaat ttagcaagag taggtgataa cagttctttt aacgtgtttg ctgcttgctc
     9661 atttcttggc tcattgtaga ctaaaatata ataagcgttt tcaaataagt gctttttagc
     9721 atttagtaca ccagatttat gactaccagc cattggatga ccactgacta aatgaatatt
     9781 atgctttaat aaattgcatt cgtgttgctg tatcattgct ttagtactac cagtatcaga
     9841 aacaataaca ccaggtttag ttggcatatc tataagctcg ctaagatatt tatttgtgat
     9901 agcaacaggt gttgcataaa taattacatc ggctttttta atagcttcac tataatttaa
     9961 acatttttca ttaataatgc cgattgattt agctttatct aactgagaag tatctgcatc
    10021 gtatgcaata atattagtat tagggttatg gtattttata ttgctagcaa gacttccacc
    10081 aattaatcca agcccaacaa ataaaactgt tgtcatataa atcaccttat ttcgaaattt
    10141 tcagaataat aacattgtaa atgagctgtt gacacagtgc aatagtaaat aaaaatcgat
    10201 aatagcatta atagataaac ggagataaat catctacaat aaagagtata gtaacacaat
    10261 ggcaacggag gggtaaatca atggaaccaa tattagaaat gattaaaaca ttaacaggta
    10321 ttaatagtcc ttcaggagac acagaagaag caattcaatt tgtcgaaaaa tatgcaaaag
    10381 acttgggtta tcaaacaaca ctaacaaata aaggtgcgtt attaataaca gtgccaggca
    10441 aaaatgatga agtacaacgc tgtattactg ctcatgttga tactttaggt gcaatggtta
    10501 aagaaattaa agaagatggt cgcttagcaa tagaattaat tggaggattc acgtataacg
    10561 cgattgaggg tgaatattgc caaattaaaa ctgatgctgg tcaaatatat acaggaacaa
    10621 tttgtctgca tgaaacaagt gttcatgtat atagaaataa tcatgaaata cctagagatc
    10681 aaaagcatat ggaaataaga attgatgaag taactacatc agaagaagat acaaagagtt
    10741 taggtatttc agtaggtaat tttgttagct ttgatccacg tacagttatc acgtcatcag
    10801 gttttattaa atctcgtcat ttagatgata aagctagcgt agcgatgata ctacaattac
    10861 taaagaaatt aaaagaagag caaataatat taccacatac aacgcaattt tatatttcta
    10921 ataacgaaga aataggttac ggtgcaaatg catcaattga ttcgaaaatc aaagaatata
    10981 ttgcattaga tatgggcgcg ttgggagacg gtcaagcatc ggatgaatat acagtttcta
    11041 tttgtgccaa agatgcttca ggtccatatc ataagcaatt gaaatcgcac ctagttaatc
    11101 tttgcaaaat aaataacatt ccatataaag tagacatata tccatattat ggttcagatg
    11161 cttcagcagc tttacatgct ggtgcggata tcagacatgg tttatttggc gctggcattg
    11221 aatcatctca tgcaatggaa cgaacacata ttgattctat taaagcgaca gagaaattac
    11281 tatatgcata ttgcttatca ccaattgagt aaacaattag tgttgacaaa tgtgaacgac
    11341 ctatgtaata taatgaacta taaaaataat tagaattttc taaagaaata gtagcagata
    11401 tgaaacgtag caaatagaaa gctaatgggt gatgggaatt agcacgccat atcttgtgaa
    11461 ttggactttg gaaaacaatt gaatgagttt tgaaagtgaa catgaattat gttaactaag
    11521 gtggcaccac ggtaacgcgt ccttacaggt atatgcgtta tgtggtgtct ttttatttag
    11581 acaaaatgta gtagttaatt aaaggtagca acagaaagtt agtggatgat gtgaactaac
    11641 accgagatta atgaaattgg gttttgtctg caacagaaaa attatatata gtaaagagtg
    11701 aactatgaat atttcgaata ttcggttaat ttaggtggta ccacgcgtca gcgtccttta
    11761 tattgataag gatgctggcg cttttttgaa aggagcgtat agaatggata tattttataa
    11821 aaaaataaaa gcaaatgtaa cgcccgaagt tttagcacaa cttcattcca agaagatcat
    11881 tttggaaagt acaaatcaac aacaaactaa aggtcgctat tcagttgtta tttttgatat
    11941 ttatggcact ttaactttag ataatgatgt attatcagta agtactttaa aagaatcgta
    12001 tcaaatcact gaaagaccgt accattattt aacgactaaa ataaatgaag actaccataa
    12061 tattcaagat gagcaactta agtcattacc atttatatct ggatatgttg ggacgtgtag
    12121 ctttgattta gtaagacatg aatttcctaa attgcaatca atacaattag aagatcacaa
    12181 gcagcacgat gtaaggttat atatggttga acaagtttat gtatttgacc attacaaaga
    12241 tgagttatat atcatcgcga cgaatcaatt ttcaaattca acaaaatcag atcttgagaa
    12301 tcgagttatt aagtctatcg aagacttaac taaaatccaa ccattcatgc ctacacaaga
    12361 ttttgatttt aaaactaaag aaattcaatc aaacatttct gaagaaagat ttatcgaaat
    12421 gattcagtat ttcaaagaga aaataacaga aggggatatg ttccaagttg tgccatcaag
    12481 aatttacaaa tatgcgcatc atgctagtca gcatttaaat caactttcgt ttcaactgta
    12541 tcaaaattta aaacgacaaa acccaagtcc atatatgtat tatcttaata tcgatcaacc
    12601 atatattgtc ggtagttctc ccgaaagttt cgtaagtgtc aaagatcaaa ttgtaacaac
    12661 taatcctatt gcaggtacga ttcaacgtgg tgagacgaca caaatagata atgagaatat
    12721 gaaacaacta cttaatgatc caaaagaatg cagcgaacat cgtatgctag ttgatttagg
    12781 acgtaatgat attcatagag taagtaaaat cggtacctca aaaattactg aattaatggt
    12841 tattgaaaaa tatgaacatg ttatgcatat cgtaagtgaa gtcacaggta aaataaatca
    12901 aaatttatcg ccaatgacag ttattgcgaa tttattacca acaggtaccg tttcaggtgc
    12961 accaaaatta cgtgcaattg aaagaatata tgaacaatat ccacataaac ggggcgttta
    13021 tagtggtggt gttggataca taaattgtaa tcataactta gattttgcat tagcaattcg
    13081 aacgatgatg atagatgagc agtatatcaa cgtagaagct ggttgtggcg ttgtatatga
    13141 ttctattcct gaaaaagaac tgaatgaaac gaaattgaaa gctaaaagct tattggaggt
    13201 gagcccatga tcttagttgt agataattat gattccttta catataacct agtggatatt
    13261 gttgctcaac atactgacgt cattgttcaa taccctgatg atgataatgt gctgaatcaa
    13321 tcggtggacg ctgttattat atctcctggt ccagggcatc cattagacga tcaacagtta
    13381 atgaaaatca tatcaaccta tcaacacaaa cccattttag gtatttgttt aggggctcag
    13441 gcactgactt gttactacgg tggagaagtc attaaaggcg acaaggttat gcacggcaaa
    13501 gttgatacac taaaggttat atcgcatcat caacatctgt tatatcaaga tataccagaa
    13561 cagttttcaa ttatgagata tcattcatta ataagtaacc ctgacaattt tccagaagaa
    13621 ttgaaaatta ctggacgtac caaagattgt atacagtcat tcgagcataa agaaagaccg
    13681 cattatggta ttcagtacca tcctgaatca tttgctacag actatggtgt caaaataatt
    13741 acaaatttca ttaatctagt gaaggaagga tgaaaaccat gacattacta acaagaataa
    13801 aaactgaaac tatattactt gaaagcgaca ttaaagagct aatcgatata cttatttctc
    13861 ctagtattgg aactgatatt aaatatgaat tacttagttc ctattcggag cgagaaatcc
    13921 aacaacaaga attaacatat attgtacgta gcttaattaa tacaatgtat ccacatcaac
    13981 catgttatga aggggctatg tgtgtgtgcg gcacaggtgg tgacaagtca aatagtttca
    14041 acatttcaac gactgttgct tttgttgtag caagtgctgg cgtaaaagtt ataaaacatg
    14101 gtaataaaag tattacctca aattcaggta gtacggattt gttaaatcaa atgaacatac
    14161 aaacaacaac tgttgatgat acacctaacc aattaaatga aaaagacctt gtattcattg
    14221 gtgcaactga atcatatcca atcatgaagt atatgcaacc agttagaaaa atgattggaa
    14281 agcctacaat attaaacctt gtgggtccat taattaatcc atatcactta acgtatcaaa
    14341 tggtaggcgt ctttgatcct acaaagttaa agttagttgc taaaacgatt aaagatttag
    14401 gtagaaaacg tgcaatcgtt ttacatggtg caaatggtat ggatgaagca acactatctg
    14461 gtgataattt gatatatgaa ttgactgaag atggagaaat caaaaattac acattaaatg
    14521 cgactgatta tggtttgaaa catgcgccga atagtgattt taaaggcggt tcacctgaag
    14581 aaaatttagc aatctccctt aatatcttga atggtaaaga tcagtcaagt cgacgtgatg
    14641 ttgtcttact aaatgcgggt ttaagccttt atgttgcaga gaaagtggat accatcgcag
    14701 aaggcataga acttgcaact acattgattg ataatggtga agcattggaa aaataccatc
    14761 aaatgagagg tgaataatat gacgatttta tcagaaattg ttaaatataa acagtcactt
    14821 ttacaaaatg gctattatca agacaaactt aataccttga aaagtgtgaa gattcagaat
    14881 aaaaaatctt ttataaacgc aattgagaaa gaaccaaagc tagcaattat tgcagaaatt
    14941 aaatcgaaga gtcctacagt taatgactta cctgaacgag atttatcgca acaaatctca
    15001 gattatgacc aatatggtgc aaatgccgtg tccattttaa ctgatgaaaa gtactttggt
    15061 ggtagttttg aaagattaca agcattgacg acaaaaacaa cattacccgt attatgcaaa
    15121 gactttatta tagacccgct tcaaattgat gttgctaaac aagctggtgc atctatgatt
    15181 ttattgatcg ttaacatctt atctgataaa caattgaaag atttatataa ctacgctata
    15241 tcgcaaaatc tagaagtgtt agttgaagta catgatcgcc atgaattaga acgtgcctat
    15301 aaggttaatg ctaaattgat tggtgtaaat aacagggact taaaacgatt tgttacaaat
    15361 gtggaacata caaatactat tttagaaaat aaaaaaacaa atcattatta tatttctgaa
    15421 agtggtattc acgatgcatc tgatgtaaga aaaatcttgc atagtggtat cgatggctta
    15481 ctaataggtg aggcgcttat gcgttgtgac aatctatctg aatttttacc acaactgaaa
    15541 atgcaaaagg tgaagtcatg atgaaattga aattttgtgg ctttacatca ataaaggatg
    15601 ttacagcggc cagtcaatta cctattgatg cgataggttt catccattat gaaaaaagta
    15661 aaaggcatca aacaattacc caaataaaaa agttagcgtc tgctgttcca aatcatatcg
    15721 ataaagtatg tgtcatggta aatcctgatt taacaacaat tgaacacgta ttaagcaata
    15781 cgtcaattaa cacaatacag ttacacggca cagaatctat tgattttata caggaaatta
    15841 aaaagaaata ttcaagcatt aaaatcacta aagctttagc tgcagatgaa aacataatcc
    15901 aaaacataaa taaatataaa gggttcgtag atttatttat tatcgacaca ccctcagtgt
    15961 cgtatggtgg taccggtcaa acatatgact ggactatttt gaagcacata aaagacatac
    16021 cttatttgat agcaggaggc attaactctg aaaatattca aacagttaat caacttaaat
    16081 tatcacatca aggttatgat cttgcatcag gtatagaagt aaatgggcga aaagatatag
    16141 aaaaaatgac agcaattgta aatattgtga aaggagatag agacaatgaa taaacaaata
    16201 caaacagaag cagatgaatt aggtttcttt ggtgaatatg gagggcaata tgttccagaa
    16261 acattaatgc cagcaattat tgagttgaaa aaagcttata aagaggcaaa agcagaccca
    16321 gagtttcaaa gagaactgga atactattta tcagagtatg taggacgcgc gacaccactt
    16381 acatatgctg catcatatac tgaaagccta ggtggcgcta aaatatattt gaaacgagag
    16441 gatctaaatc atacaggcgc ccataaaatt aataatgcgt taggtcaagc gttgcttgct
    16501 aaaagaatgg gcaagaagaa gcttgttgct gaaactggtg cgggtcaaca tggtgtagct
    16561 agtgctacgg ttgctgcatt atttgatatg gaacttgttg tctttatggg aagtgaagat
    16621 attaaaagac aacaacttaa tgtatttaga atggaattac ttggtgcaaa ggttgtggca
    16681 gttgaagatg gtcaagggac tttatcggat gcagttaata aagcattgca atattgggta
    16741 agtcatgtag atgatacaca ttatttatta ggttctgcat taggtccaga cccgttccca
    16801 acgattgtta gagattttca gagtgtgatt ggtaaagaaa taaaatcaca gatattgaag
    16861 aaagaaggtc gacttccgga tgcaattgta gcatgtatcg gtggtggctc aaatgcaatc
    16921 ggtacatttt atccatttat taaagatgat gttgcattat acggtgttga agccgcaggt
    16981 caaggcgatg atactgataa acatgcactt gcaattggca aaggatcacc tggcgtatta
    17041 catggtacta aaatgtattt aattcaagat gaagatgggc aagtgcaact agcacattct
    17101 atttcagcag gacttgatta tcctggtatt ggaccagaac attcttatta ccacgacatt
    17161 ggtagagtaa cttttgaaaa tgctagtgat acacaagcaa tgaatgcttt aatcaacttt
    17221 acaaaacatg aaggtattat acctgcaatt gaaagtgcac atgcactgag ttatgttgaa
    17281 agactagcgc ctacgatgtc gaaagaagat attattgtag taactatttc tggacgtggc
    17341 gataaagata tggaaacaat tagacaatat atggtagagc gaggtcttgc aaatgactaa
    17401 attatttata ccttatatta tgggcaataa agatttgatt gaaaatgcaa cattgttgag
    17461 tgaaaatggt gcagatataa ttgaaattgg agtacctttc tctgatccgg ttgctgatgg
    17521 tccagttatc atggaagcag gtcaacaagc gattaaacaa ggcatcacga tagattatat
    17581 tttcaatcaa ttagaaaaac atggtgatca aattaagtgt aactatgtat taatgacgta
    17641 ttataatatt atttgtcatt atggagaaca agcgtttttt gaaaaatgtc gagatactgg
    17701 tgtctacggc ttaattattc ctgatttacc atatgaatta tcgcagcgtt taaaacaaca
    17761 atttagtcac tatggcgtca aaatcatatc gttagttgcg atgactactg atgacaaacg
    17821 tataaaagat atcgtatccc atgcggaagg ctttatttat actgtgacga tgaatgcgac
    17881 aacagggcaa aacggtgcgt ttcatccaga attaaaacga aaaattgagt caattaaagc
    17941 gatagccaat gtgccagttg tcgcaggatt tggtataaga acaccacaac atgttgcaga
    18001 tataaaagag gttgcagatg gcattgtcat tggtagcgaa atcgttaagc gatttaaatc
    18061 taacacgcgt gaggaaatca ttaaatattt acaatctatc caacaaacat tgaataatta
    18121 agtttacttg atttaaaaaa attaggcgaa tactgtttga aaaagtgaaa aacggtgaat
    18181 tataaaattg aatagaaatg atacaaaagt aatatgagca aacccaaacg ttcatattac
    18241 tttttttgaa attgtattca aaaatctaaa tattactata aaagtatacg caattaaagc
    18301 gtttatgttt tagttttaac attaactatt gtatacttat ttagattaga tttattattt
    18361 ttgacatttg cagaggggaa atagaaaaac tgcaaatacg gaaatgaaat taattaacga
    18421 gagacaaata ggagtaatga taatgaagtt tacaaattta acagctaaag agtttggtgc
    18481 ctttacagat agcatgccat acagtcattt cacgcaaact gttggccact atgagttaaa
    18541 gcttgctgaa ggttatgaaa cacatttagt gggaataaaa aacaataata acgaggtcat
    18601 tgcagcttgc ttacttactg ctgtacctgt tatgaaagtg ttcaagtatt tttattcaaa
    18661 tcgcggtcca gtgattgatt atgaaaatca agaactcgta cactttttct ttaatgaatt
    18721 atcaaaatat gttaaaaaac atcgttgtct atacctacat atcgatccat atttaccata
    18781 tcaatacttg aatcatgatg gcgagattac aggtaatgct ggtaatgatt ggttctttga
    18841 taaaatgagt aacttaggat ttgaacatac tggattccat aaaggatttg atcctgtgct
    18901 acaaattcgt tatcactcag tgttagattt aaaagataaa acagcagatg acatcattaa
    18961 aaatatggat ggacttagaa aaagaaacac gaaaaaagtt aaaaagaatg gtgttaaagt
    19021 aagattttta tctgaagaag aactaccaat ttttagatca tttatggaag atacgtcaga
    19081 atcaaaagct tttgctgatc gtgatgacaa attttactac aatcgcttaa aatattacaa
    19141 agaccgtgtg ttagtacctt tagcgtatat caactttgat gaatatatta aagaactaaa
    19201 cgaagagcgt gatattttaa ataaagattt aaataaagcg ttaaaggata ttgaaaaacg
    19261 tcctgaaaat aaaaaagcac ataacaagcg agataactta caacaacaac ttgatgcaaa
    19321 tgagcaaaag attgaagaag gtaaacgtct acaagaagaa catggtaatg aattacctat
    19381 ctctgctggt ttcttcttta tcaatccatt tgaagttgtt tattatgctg gtggtacatc
    19441 aaatgcattc cgtcattttg ccggaagtta tgcagtgcaa tgggaaatga ttaattatgc
    19501 attaaatcat ggcattgacc gttataattt ctatggtgtt agtggtaaat ttacagaaga
    19561 tgctgaagat gctggtgtag ttaaattcaa aaaaggttac aatgctgaaa ttattgaata
    19621 tgttggtgac tttattaaac caattaataa acctgtttac gcagcatata ccgcacttaa
    19681 aaaagttaaa gacagaattt tttaggaagg gaattatcaa aacatgaaat ttacagagtt
    19741 aactgttacc gaatttgaca actttgtaca aaatccatca ttggaaagtc attatttcca
    19801 agtaaaagaa aatatagtta cccgtgagaa tgatggcttt gaagtagttt tattaggtat
    19861 taaagacgac aataacaaag taattgcagc aagccttttc tctaaaattc ctactatggg
    19921 aagttatgtt tactattcga atcgtggtcc agtaatggat ttttcagatt taggattagt
    19981 tgattattat ttaaaagagt tagataaata tttacagcaa catcaatgtt tatatgttaa
    20041 attagatccg tattggttat atcatctata tgataaagat atcgtgccat ttgaaggtcg
    20101 cgagaaaaat gatgccctag taaacttgtt taaatcacat ggttacgagc atcatggctt
    20161 tacaactgag tatgatacat cgagccaagt acgatggatg ggcgtattaa accttgaagg
    20221 taaaacaccc gaaacattga aaaagacatt tgatagtcaa cgtaaacgta atattaataa
    20281 agcgataaac tatggtgtta aagtcagatt ccttgaacgt gatgagttca atcttttctt
    20341 agatttatat cgtgaaactg aagagcgtgc tggatttgta tcaaaaacag atgattattt
    20401 ttataacttt attgacacat atggagataa agtattagta ccattagcat atattgacct
    20461 tgatgaatat gtgttaaagt tgcaacagga attgaatgac aaagaaaatc gtcgtgatca
    20521 aatgatggcg aaagaaaaca aatcagataa gcaaatgaag aaaattgcag aattagataa
    20581 gcaaattgat catgatcagc atgaattatt gaatgcaagt gaattgagca aaacggacgg
    20641 cccaattcta aaccttgctt ctggcgttta ttttgcaaat gcatatgaag tgaattattt
    20701 ctctggtggt tcatcagaaa aatataatca atttatggga ccatacatga tgcattggtt
    20761 tatgattaac tattgcttcg ataatggcta tgatcgttat aatttctatg gtttatcagg
    20821 tgattttacg gaaaacagtg aagattatgg cgtataccgc tttaaacgtg gatttaatgt
    20881 acaaatcgaa gaattaatag gggatttcta taaaccaatt cataaagtga aatattggtt
    20941 gttcacaaca ttggataaat tacgtaaaaa attaaagaaa tagatgtaaa tgatgttagg
    21001 gcttatagtt attgatacta taggctcttt tttatatgtt ttttaataaa gcctcgaata
    21061 atgatatatt aaatgacaag tttaacaagt gattgcaata aatataaata tgaattgcac
    21121 aacctatttg taaatgattg agttgagtat actttttaat tcattcaata tgactcaaaa
    21181 caagccaata tgtattatat ttttggtgaa taaaccttag cataacaatt tatgtctgat
    21241 tatatatgtg tgtgtttgca agtgtgtgat atcaaatgtc atatttgtct aattgtaatt
    21301 tataaaatgc atgagcaatg attgctgtaa caaattctag caaagtattc agtttgtatt
    21361 ttagaaataa aaatgatgat agatgtatga tcgatataaa agatttaata tgcaccaaaa
    21421 tgataacgtg agtaattggt ttaatgaaac ataacatgaa tgtaaattta gcttactgac
    21481 ataaacacta cacaaacata cagttatata agtcccctta tggaatttca cattctagtt
    21541 tacataatat atattatagg aagttatatg tgtgtaacgc aaaaggtacc ctacatcata
    21601 atcattatct aatatcgtca cataacttac ttatgctata atcatggtat tatattgttt
    21661 ggagtgattt gatgagattt gtctttgata ttgatggtac gctttgtttt gacggccgat
    21721 taattgacca gactattatt gatacattgt tacaattaca acatgatggt catgaactta
    21781 tatttgcatc agcacgtccg attcgtgatt tgttgccagt tttaccatca gtatttcatc
    21841 agcacacatt aattggcgca aatggtgcta tgatttcaca gcaatcaaag atttctgtta
    21901 tcaaaccaat tcatactgat acatatcatc atatcttcaa aataattcaa aagtatgagt
    21961 tagattatat tattgatgat gattggaatt atgctgcaca acttgacgct gagaacgcga
    22021 tttttgagcg tttagatcca cataagctgg ccagttgtat tgatgttgca aatatcgaca
    22081 caccaatcaa gattatttta ttaaatatag acccggcaca aattacaact atattagacg
    22141 agctagataa ataccatcaa gaattggaaa tgattcacca ttcaaatgag tataacattg
    22201 atataacagc gcaaaatatt aacaaatata ctgcattaca atatatattt gatgcagatg
    22261 ttaaatatat agcatttggt aatgaccaca atgatattgt catgttacaa catgctagta
    22321 gtggctatat tataggacca tcagaagcat acacacacgc aatattgaaa cttgataaaa
    22381 tcaaacacat caataataat gcacaagcta tttgcaaagt cttaaaatca tataaataaa
    22441 aacaccccta tcaaatgata atcattatca atcgataggg gctattttaa taaaattcgt
    22501 cctcgaacat ttcttcctct tcatctaatc caaataattc tgccatttct ccatgttcaa
    22561 ttaacatgtt taaatatgca tcgcggagtt cttcttcact catatcatta atcatttctt
    22621 taagactatc aatccacata tttctgcgta attgatagtc ttcttcaact tcgtttaaca
    22681 tcattatatg tttatttgct gcttctggac tagctgtaaa gagtaatgca atcatatgtt
    22741 tacatatcac tcgtcttcca tcagcatgag gacaattaca tatggatttt ctaggatgtt
    22801 ccatatcaat ataacaacga tatactttgt tgccactgcc ctttacttca gcctcatgct
    22861 gcgtttctga aaatgatttt aagttaatga cgcattcact ttgataataa ttaaagcctc
    22921 tttctataga acgaatactt gcaatatcaa gtaatcccat taatgatact cctttttatt
    22981 attattttta aataaagaaa ataaaataga taagtgtcta gattaaaata cttgatttat
    23041 ctatatttta taacaagtct agaattatcg cattcttaaa taactaatat gaaaatgctt
    23101 gcactaattc ttttgtataa gggtgtctat caacattaaa taattcctct attgcaaaat
    23161 catcgactat catgccatcc ttaagaacga taattctatt aactaagcgt tgtaacacgg
    23221 ataaatcatg agaaataacg ataaaatgat ttaagttcgt aatcgtttgc gcttttaata
    23281 tattgattac attttgttca gctataacat ctaaatttga agttatctca tcacatatta
    23341 aaacgcgagg ctgtgctaat aacgaacgca tgacattaaa tctttgtaat tgtccgccac
    23401 tcacttcgct tggtaattta gtcaataatt gcgcgtttaa ctcaaaagta gataaatgtt
    23461 gtaataataa ttgatcctga gcagtattat cagttagacc tctgtaataa tataacgctt
    23521 cttttaatga ggtctcaatc gtccaatcag ggttaaagct agttaaaggg tgttggaaaa
    23581 tcggtaacac agcattgtca cttaagtaaa tctctccttt aacaggttta aacaagccaa
    23641 gaaccaatga agcgagcgta cttttaccac agccactttc gcctaaaata ccaacatttt
    23701 ctccatcagg tatagtaata ttgatatctt gtagcaccat ctgcttttta ttataaccaa
    23761 aagtcacatg ttttaactca atcatagtaa tccctcttta attgtgttct atatttaatt
    23821 agacgttcag tatacggatg caaatgctca tacttgaaat gattaatatt acctcgttca
    23881 atgatttgac cttcttttaa aacataaatg tactgacaat atttcaatac atgacttaag
    23941 ttatgtgtga taataaataa tgtttgacca tgttctaata caatatgctg taataaatcc
    24001 atcacttgat taccgttcaa agcatccaat gatgcaactg gttcgtctgc aatgattaat
    24061 ttaggctcca acatgagaac gcttgctatg tatacgcgtt caagttggcc cccagaaagt
    24121 tggaaactat atttatttaa tatatctttg ctttgtaaat taacccacga caaagcctta
    24181 tcaactttgg acaaagcctc ttctttacta cctttataat gcttacgata aatcgcagtt
    24241 aactgtttac ctaatttagt atggtcgtta aaactttctg cataattttg agaaatatag
    24301 ccaattgtat gaccataata ttgactcaat ctactaacat tttccccatc aaattggtac
    24361 gaatcatacg tgcagcttaa atcaaatggt aaatattcaa gtaaagcttt agcaatcaaa
    24421 ctttttccag cgccgctctc tccaatcaag gcattaatct gttgactaaa aattttcaaa
    24481 tcaatccctt taataagaga tttctcacta gtattcttta ttgttaaatt ttgtatatca
    24541 atgagactca tcatattcac cccgttgttt cagcaatcta tctcttagtg catcaccggt
    24601 taaattaaaa attaaaatag ttatagcaat gactgaagca ggtgcaatca acataattgg
    24661 atgagacgaa ataaaatcac gaccttgttg caacatagcg ccccactctg gtgttggcgg
    24721 ttgtgcacct aacccaataa atgatagtga acttatatat agaatgattt taccgaaatc
    24781 aacgaccatc aaaacgataa tagccggtat aattttaggt gttaaatgac gtattaatat
    24841 tgttcttgtt ggtacatgaa ataattgtgc catttttata taaggcttat tcatttcgct
    24901 attaactata cttctagtca accttgtgta attcatccat tttattaatg taattgagat
    24961 aactaaattc cataaagatg gttgaaaaaa acttgctaaa gcaatcatga tgataaattc
    25021 tggaatactt agaccaacat caataaacct taacactaat cgttcaatcc accctttttt
    25081 gtatccggca aatagaccta gtgtaacacc tatgacaacg atagctatta atgttaaaac
    25141 agtaacaaac aatgttgaac gtgcaccgat aataattcgg gtaaataaat ctctcccata
    25201 atcatcagtt cctaataaat gcaaccaact aataggttca aaagtttgtg ataaattgac
    25261 tttggttgca ttttcactac tgacaaagaa ttgcagtaca attaccacaa aaataaatgc
    25321 aacgaataca aaaaatatca ggttattctt tgaaaatatt ttatgcatga cggtcactac
    25381 tttctgatat caatggtgta ttggttttga tttttggatt tcctaattgt aaacgctgct
    25441 tcggatcaag taataacgtt aataaatcag caatcgtatt gataataaca acgaagaagc
    25501 caataaataa cacgcatcct tgaataacag gataatctcg agatttaata ctatccatta
    25561 atagataacc aataccaggt atatcaaata aattttcaat cactacagta ccacctatta
    25621 gactgccaag tgaaatccct agtaatggga taatcggcaa aattgttggt tttagtaaat
    25681 catgaattaa aatataacgt tcattcatac cgcgtaatct tgatgcttgt acgatattac
    25741 tttgcaataa catcaataaa ttagaacgca ctaaacgaat gatgtatgca cacataccta
    25801 aagatagcgt gattacaggt aatataaact gacttagtat aacgctatct atattcatta
    25861 aatttgtgac aataaataat aaaataatac cgataaagaa cgctggtaaa ctaatcgata
    25921 gtgttgagat cactctaatc actttatccg tccacttatg aaatcgtttg gctgctataa
    25981 tgccgagcgg tatagatatg cataacgaca ctactaatgt tgaaaatgat atgagtaatg
    26041 ttatgggtgc atagttgaat aatatctgtg ttaccggttc ttttgattca aaactttttc
    26101 ctaaattaaa atgtaataaa tgattcatcc aatgccacca ctgtaccaat aaagaatcat
    26161 ttaatcccaa tttatctttg gttgcattta tttgttccgt cgacacttgt gctacatcaa
    26221 gatgtaatat tttatcaaca ggattgcctg gtgataattt cattaaaatg aatgtaagtg
    26281 tagaaataac aaataaaaca actatcattt gcatcagtct atacaacata gactttatta
    26341 tgaacataat agtccccctc cttgtgtaag ttactaacac tttcttttta catgagaatg
    26401 gcgcatgtat atgcaactta catattaaga actaacgttc attatagtat tatccataaa
    26461 gaaattgaag tatatttaat tttttaacaa aatcattata aaatataata ttttgaatca
    26521 agtcaaccat gtaaaatata aaaaagtcaa aacaaaaaca actatagcac tgtattccat
    26581 ctctttcgaa ataattgtta ctgcagtgta acttaaaagt cgatgatttt gtgcatatag
    26641 ttgtcgaata ttatttttta tctttacggc gaagttcagc gccctcatag ccgtattttt
    26701 caatttgctt ttctaattta cgcgcttttc tttctttacg ccaatttcta gtaaaatacc
    26761 ataatagaaa actaattaat aaactcataa tcgctaaaaa tgcagcgtat cctaataatg
    26821 gttgatattt tatatcttga aaatttggaa taaaaaatgc aagcacacct aatataacaa
    26881 atgtaattac tgcagataca aaccatttat ttaaaactaa gcaacagaat attgttaata
    26941 aaatcattat taatgttgtg atccataaat aattaggcat atcgaataat gtcatattca
    27001 ttctcctttt atttcattac tttccttgta tacattttat tataaatttt taaaaactta
    27061 aacaatagca gtcagtttca agcaatattc tatctactaa tagaaaaatc attgttcctt
    27121 gcgacatgga aatcgtaaca ttatcgttta ggagacaaaa ttatgtataa tgaatgtatt
    27181 ataccaaagg agtgattata tgtctcaagg tttaccttta agagaagatg ttcctgtttc
    27241 agaaacatgg gatttagtag acttatttaa agatgatcaa caatattatg aaagtattga
    27301 cgctctagta caacaagcaa atcaatttca tcatacatat gcaacaacat taaattcaat
    27361 cgaacaaatt aatactgctt tagctgaatt agaaaatatt ttaattgcct tagatcgctt
    27421 aagtaattat gcagaactac gtttaagtgt agatactagt aatatcgagg cacaagtatt
    27481 gagcgctaaa ttatctacta catacggtaa aattgttagc caattatcat ttgtagagtc
    27541 agaaatactt gaattaccag aagaaatact tcaacaatta gaagaatcat gtccatatca
    27601 acactatatt aaacagttaa taaaacaaaa gccattccaa ttatctgcgt cggtagaaca
    27661 agtattagca actttatcac ctacgctaaa cagtccttac gatttatacg gcacgacaaa
    27721 aatgctagat attacattcg attcatttga acatgatggt acaacgtacc ctgtcgacta
    27781 tgctacgttt gaaaatgatt atgaagataa taaagatcct gagtttagac gtaaaagttt
    27841 caaatcgttt agcgatggga ttcgaaaata tcagcatact accgcggcta catataatat
    27901 gcaagtacaa caagaaaaaa ttgaagctga tttacgtgga tttgaatcag tcatcgatta
    27961 tttattacat agtcaagaag taacgcgtga tatgtttgac cgtcaaatcg atatgattat
    28021 gcgtgacttg gcaccagtta tgcagaaata tgctaaactt ttacaacgta ttcacggatt
    28081 agataacatg cgttttgaag acttgaagat ttctgtagac cctgattatg aaccagagat
    28141 ttcaattgaa gactcaaaaa attatatttt cggtgcgtta agtgttttag gtgatgacta
    28201 tacaaacatg ttacgtgaag catacgatca gcgatggatt gattttgcac aaaataaagg
    28261 taaagataca ggcgcatttt gtgcaagtcc atactttaca cattcatatg tgtttatttc
    28321 ttggactggt aaaatggctg aagcatttgt cttagcacat gaattaggtc atgcaggtca
    28381 ttttacatta gctcaaaaac atcaaccata tcttgaatca gaagcatcaa tgtactttgt
    28441 tgaagcccct tctacaatga atgaaatgtt gatggccaat tatttattta acacaagtga
    28501 taatccaaga tttaagcgtt gggttattgg ctcaatttta tctagaacat attatcataa
    28561 tatggttacc catttattag aagctgctta tcaacgtgaa gtgtatcaca aagtagatca
    28621 aggtgaatct ttaaatgcgc cgacattaaa tgaaataatg ctaaatgttt ataaacaatt
    28681 ttttggagat gcagtagaca tgactgaggg tgctgaatta acatggatgc gtcaacctca
    28741 ttactatatg ggattatatt cgtatacgta ttctgctggc ttaacaatcg gaactgtcgt
    28801 ttctcaaaag attaaaaatg aaggccaacc agctgttgat gcttggttag aaacattgaa
    28861 aaaaggtggt agtgtatcac ctgtcgaact tgcaaacatt gcaggtgtag acattactac
    28921 agaacagcca cttaaatcta caattcaata tatttctgat ttagtcgatg aagttgaaaa
    28981 attaacagat gaaattgagc aagcaaataa ctaagatgcg agacaattta tagtagaaca
    29041 atataaacca agattttcac attactaatt gtgagaatct tggttttttt gagggtttat
    29101 gaataataga aatatatatt tatcgtaagg gattttatag taataacttt aagttattgt
    29161 tcgtaatgtg tacctgttaa ataaaaataa acactttcag cgatgttaat aatatgatca
    29221 ccaatacgtt ctaaatgtct tgctgctaaa tgagcttgtg cagcgacaaa tggatcgtta
    29281 tcaataagat acgttgcgtt aataatatga ctatataagt catcgatatc ttcatcacgc
    29341 tcaattattt ctcttattaa tacggtatct ttctttttaa atgcttgatc taagtccttt
    29401 aacattaaca tagctaattt acccattgtc tttaaacggg ttaacacata atcatctgta
    29461 atctttgtac gcaatcgaat attggcaata ctcgaggcat tatctcctat tctttctaaa
    29521 tcggaggcga tttttaatga agaaatcatc atacgcaaat cactcgcaat gggctgttgc
    29581 tttgtaatta acatgataac tcgctcatta atatcataat ttaattgatt gatatgttta
    29641 tcgtttttaa ctgtttgtcg tgcaaagcct ctatcgtcaa tacttaatga ttttatacca
    29701 ttttcaatac tcacatagac atttgcacct aaccgacgta attcttttat taaatcatca
    29761 agttgctcct gatatcgttg tctaattatt gccattatat atcaaccaaa ccttcctgaa
    29821 atataatctt ctgttttctt gtttgatggg ttagagaaaa ttttatcagt atcatcatat
    29881 tcattgacat aaccatttaa gaaaaatgca gttttatctg atacacgagc tgcttgttgc
    29941 atattatgtg taaccataat aattgtatac ttttctttta gttcttgaac caactcttct
    30001 actcttaatg ttgagattgg atctaatgct gatgtcggtt catccattaa aatgacttca
    30061 ggttcaattg ctaaacaacg cgcgatacaa acacgttgtt gttgcccacc ggataaacta
    30121 tatgcatttg tgtgcaacct atcctttaat tcatcccaaa ttgcagcgcc acgtaatgat
    30181 ttctcaacga tttcatcaag aactttttta tttttaatac cgtgaatctt tggaccgtaa
    30241 gtaatattat cgtatattga ttttggaaat ggattaggtt gttgaaagac catgcccaca
    30301 tttgtacgta attgttcttt agaatatttt tgatcaaaaa tgtcttgatc tcgatataat
    30361 attttaccag ctgttttcac agaaggtact aactcaacca ttcgattcaa agttttaata
    30421 tatgttgatt tgccacaacc agatggacct ataatggcag taatttggtt ttcataaata
    30481 tctaaattaa tattttgtaa tgcatgattt tcgccatacc ataagtctaa attttgtgtt
    30541 gaatatataa cagaatgtga gtttgtatca tctggtgttt tatgatgact ttgtgagaca
    30601 tcaaacgtat gactttgaga tatttgttta gtttgtgcaa gtgtttgcgc catatttaaa
    30661 actccctttt taatagataa atgagattga ttgtttaaat tagaattttt tactaaattt
    30721 gttacgtaaa ataatcgcaa cgccattcat taagattaag ataactagta aaacgataat
    30781 gcctgccgat gcaacattct ggaattcttc ttgaggcatt ttcgcccaag taaatatttg
    30841 gataggtaat gctgaaaatt gatccaatat acttctaggt gttgccaata atatagtcgg
    30901 tataccgatt agcacaagtg gcgctgtttc tcccagtgct cttgaaagag acaaaatgaa
    30961 tccagttaaa ataccaggta acgctgctgg taagacaaca cgtcttatcg tttgccattt
    31021 attagcacct aagccgtaag aagcttcgcg tactgagtta ggtacagctc taattgcttc
    31081 ctgacttgaa acaataataa ttggtaatat cagtaaggtc attgttagcg ctgctgccaa
    31141 tatactgtta cccattttca aggcttcaat ccctgcacca ccaacgaaca aagtataacc
    31201 taataaccca aatacaactg atggtacacc agctaaattg gaaatactga ttttaacaaa
    31261 ctgagtaaat ttgttgtttt tcgcatattc ttctaagtat atagctgtac ctattcctag
    31321 gatgattgat aatggaatga tacttaacat taaccaaagt gaaccgatta acgcgccttt
    31381 aacgccagcc atagatggtg ttgaagaaga aaaattagtg aaaaactgta aatttaaatg
    31441 acttacccct ttaatcaatg tttgagttaa caacgcaata agtacgacaa gtcctaataa
    31501 tgtacatgct aaaaatatga gtttgaacac tttattttta accgttctgg atgataaatg
    31561 tttttggaca agttgttgat cgacgagtga ttgtctatta ttatctgtcg tttccatatt
    31621 aatactcctc cctaaaacgc ttagaaatcc actgagaaag taaattcatg attaaggtaa
    31681 agataaatag tgtgaaccct acagcataaa tactgtaata aatatttgat ccaaatgttg
    31741 catcacctgt cgctatctca acaatatatc cagtcattgt ttgaatcgaa cttgttaaac
    31801 ttaatgaagc tgttggcgaa ctacctgccg ctaatgatac aatcatcgtt tctccaattg
    31861 ctcttgaaat cgcgagaacg attgaagcta caatacctga tgttgctgcg ggaagtacga
    31921 ctttagttgc tacttctaat ttagttgctc caagtccata ggcaccttct cgaattttat
    31981 ttggtacaga tgccattgca tcctcactca aacttgtgat gagagggaca atcataatac
    32041 cgacaactaa gccgggactt atagcattaa actctccaag acctggtatg aaagatctta
    32101 atactggtgt aacaaaggtt aatgcaaaga aaccaaacac aattgttggt attcctgcta
    32161 aaatttctaa tatcggttta attatgcgtc gtgcacggtc acttgcatat tcacttaaat
    32221 aaattgctgc accaagcccg actggaactg caaatatagt cgcaataact gtgattttta
    32281 aagtccctat tatcaatgcc cagataccaa acttagggtc tgaaccggta ggattccaag
    32341 tagtagaaaa tagaaattca gttattggaa ttctggtgaa aaaagtgatg gtttctaaaa
    32401 gcaatgtgat taatatacct agtgttgtta aaatggaaat cgctgaaatt gcggctaaaa
    32461 taactggtat aattttgtca ttatgctttc cttttttatt attatttttt tcgattaaag
    32521 ctttaacatt agtagatgaa gtcataatca aacctctttc tattactccc aaaataatta
    32581 gatttgtaaa ataatgatga accactattt taagttgata agcttgtaga ctaactgaat
    32641 gtcaaaatca gcgtctcgat aaatcaagct ttgtactcta aataatgatt caccatcaaa
    32701 atcatgtttc aagacacatt tgaaattgcg tcttattatt ttttgtcttc agacttttta
    32761 tcatcagatt tcttgtcgtc tgatttttga tttttatcaa taaatgcttt taaatcatct
    32821 aattgtgatt tgtatgtttt ctctggtgct gctacatatc cagcttcttc agctgcttta
    32881 cctttatctt ctaagacgaa tttgataaat tctgacatta ctttattatc tttcaatgct
    32941 ttttcattta cataaatgaa taatggtcta cttaatgcat aagagttatc ttgaattgtt
    33001 tttttcgtag gctctgttgc tttaccattt tcatctttga ttttaacttc ttttaattta
    33061 tctttatttt gtacgtagaa gttatatcca aagtatccga ttccctcttt gtttttcgtt
    33121 acagaagaaa cgatagcatt tgtatcagca tttttttctg ctttaatatc ttctttattc
    33181 attacttcat tttcaaagaa gtcataagta ccatgacttg agtttggtga tacagcattt
    33241 atttttttat ctggccattt actattaaca tctttccatg ttttagcttt tccagaataa
    33301 attgctttta attgctgttt gtctaattca tctacaaaat cattttcttt atttacagca
    33361 accgttacac catcttgcgc aattttgaat tctttgtatt tgatattctt atcttgtaat
    33421 ttttgcttct cttcatcttt aattggtcta gaagcatcag cgaagtcgat atctcctgca
    33481 atgaattttt ggaaaccagc acctgtacca gcttgtcctg ctgagatttt agcatccgag
    33541 tgatcttgag cccatttttc atttaatttc tccacaattg gtgctactgt tgatgagcca
    33601 tcacctttag cttccccttt taaatcacta ttaccactgc caccattacc gccaccacaa
    33661 gcacctaata atagtgttgc acctaaagct gtagtaccaa caaattgcca ttttttcatt
    33721 gaaatatcct ccctgtatga acaacaaata gtatttgtat tgattacatc tttatcttaa
    33781 ccttgaatta ttaacctcat ttttagaaac tgtaaatgtt acataaaaaa ttgttaagaa
    33841 aatgtaaata ttaatttttg ttaatctaaa aaatattgct tcattagtaa aattaaaatg
    33901 cttcctgaaa aatcatcgaa atttaaatat gaatacaaaa a
//
LOCUS       DALAGD010000018        33899 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.018, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000018 DALAGD010000000
VERSION     DALAGD010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 33899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33899
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.018"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            233..586
                     /locus_tag="O2701_000662"
     CDS             233..586
                     /locus_tag="O2701_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HCZ4292571.1"
                     /translation="MFSIGSAILHFVIGGIAVALASIIADKVGGKLGGIIATMPAVFL
                     AAIIALALDHRGTQLVEMSMNLSTGAIVGILSCILTVFLTSLYIKHKGYRKGAIFTVV
                     CWFVISLAIFSIRHL"
     gene            654..1049
                     /locus_tag="O2701_000663"
     CDS             654..1049
                     /locus_tag="O2701_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HCZ4292572.1"
                     /translation="MKLTLMKFFVGGFAVLLSYIVSVTLPWKEFGGIFATFPAVFLVS
                     MFITGMQYGDKVAVHVSRGAVFGMTGVLVCILVTWMMLHMTHMWLISIVVGFLSWFIS
                     AVCIFEAVEFIAQKRLEKHSWKAGKSNSK"
     gene            1309..1878
                     /locus_tag="O2701_000664"
     CDS             1309..1878
                     /locus_tag="O2701_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCZ4292573.1"
                     /translation="MNLSKQIKKYRERDGYSQEYLAEKLYVSRQSISNWENDKSLPDI
                     HNLLMMCELFNVTLDDLVKGTIPFVPDIKAQRSLNLWTYVMLIFMTLAAILMGPLVVY
                     WNWTWGVTVAIILGIGFYASMKIEDLKKVHKMDNYDRIVAFMNGKDPSEVQTTKARNT
                     MTNALSIISVIGILSLIIFLSVYLANKFL"
     gene            complement(2005..2205)
                     /locus_tag="O2701_000665"
     CDS             complement(2005..2205)
                     /locus_tag="O2701_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCZ4292574.1"
                     /translation="MNNGTVKWFNAEKGFGFIEQENGGDVFVHFSGIASDGYKTLEEG
                     QKVTFEITEGQRGDQAVNVQTV"
     gene            complement(2598..2789)
                     /gene="vraH"
                     /locus_tag="O2701_000666"
     CDS             complement(2598..2789)
                     /gene="vraH"
                     /locus_tag="O2701_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter activity
                     modulator VraH"
                     /protein_id="HCZ4292575.1"
                     /translation="MKFRELVKQSYEDLKNLTVTWYNVIALTILVIVLSSFTTPMIGI
                     PAGLLGGSYYLKRREEKGK"
     gene            complement(2818..3144)
                     /locus_tag="O2701_000667"
                     /pseudo
     CDS             complement(2818..3144)
                     /locus_tag="O2701_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012656145.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            3159..3245
                     /locus_tag="O2701_000668"
                     /pseudo
     CDS             3159..3245
                     /locus_tag="O2701_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790915.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3247..3744
                     /locus_tag="O2701_000669"
     CDS             3247..3744
                     /locus_tag="O2701_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCZ4292576.1"
                     /translation="MIINLETQQIHFDLEHEIPYFQAPEKNRIRLDIDVLNKKQISNI
                     INVIFNNQSKTKCTFLSEYLYPVKFREKTRIGRFFNITNWYEEIHSTDEKYVIATIKN
                     IKNKDLIDYSLYIKKGLVEPFIVFYNQENIFYISNAVIDIISVSKDTLDYIKSEFNDH
                     CASPY"
     gene            complement(3804..4304)
                     /locus_tag="O2701_000670"
                     /pseudo
     CDS             complement(3804..4304)
                     /locus_tag="O2701_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532440.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(4352..4543)
                     /gene="vraH"
                     /locus_tag="O2701_000671"
     CDS             complement(4352..4543)
                     /gene="vraH"
                     /locus_tag="O2701_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter activity
                     modulator VraH"
                     /protein_id="HCZ4292577.1"
                     /translation="MKFRELVKQSYEDLKNLTVTWYNVIALTILVIVLSSFTTPMIGI
                     PAGLLGGAYYLKRREEKGK"
     gene            complement(4636..6516)
                     /gene="vraE"
                     /locus_tag="O2701_000672"
     CDS             complement(4636..6516)
                     /gene="vraE"
                     /locus_tag="O2701_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter permease
                     subunit VraE"
                     /protein_id="HCZ4292578.1"
                     /translation="MTFNHIVFKNLRQNLKHYAMYLFSLFFSIVLYFSFTTLQFTKGV
                     NNDDSMAIIKKGALVGSIFLFIIIVIFLMYANHLFVKRRTREFALFQLIGLTRQNILK
                     MLALEQMIVFLITGVVGVLCGIAGAQLLLSIVSKLMSLSINLSIHFEPMALVLTIFML
                     IIAYVLILFKSALFLKRRSILSMMKDSIKTDATTAKVTTAEVISGVLGIAMIALGYYM
                     ATEMFGTFKALTMAMTSPFIILFLTVVGAYLFFRSSVSLIFKTLKKSKNGRVSITDVV
                     FTSSIMYRMKKNAMSLTIIAIISAVTVTVLCFAALSKSNTDQTLTSMAPNEFNVVATQ
                     DAKQFETKLSQQQITFSKNAYETITVDNVNDQVITLENGSDSGRTNSILSANNKLTGN
                     NAIITNTKSLPNIINIHLNKDLVVKGTKNETFRVTQEDKGKVYPLNLSFNSPVIEVSP
                     EKYQQLKTQNNVHTFYGYDIKQTSQKEKAQAIAKQFGDKVITYDEMKKEVDATNGILI
                     FVTSFLGLAFLVAAGCIIYIKQMDETEDELSNFRILKRIGFTHTDMLKGLLLKITFNF
                     GLPLLIAILHAVFAAIAFMKLMGNISFMPVIVVIVVYTLIYITFALIAFVHSNKLIKK
                     TI"
     gene            complement(6506..7264)
                     /gene="vraD"
                     /locus_tag="O2701_000673"
     CDS             complement(6506..7264)
                     /gene="vraD"
                     /locus_tag="O2701_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter ATP-binding
                     subunit VraD"
                     /protein_id="HCZ4292579.1"
                     /translation="MTILSVQHVSKTYGKKHTFQALKDINFDIQKGEFVAIMGPSGSG
                     KTTLLNVLSSIDQISSGSVIANGQELNKLNQKALAKFRKESLGFIFQDYSILPTLTVK
                     ENIMLPLSVQKMSKATMEENYKAITTALGIYDLGNKYPSELSGGQQQRTAAARAFVHK
                     PQIIFADEPTGALDSKSANDLLQRLEEMNKSFDTTIVMVTHDPVAASFAERVIMLKDG
                     QIHTQLYQEGRSKQAFYEDIVHLQSVLGGVSNDI"
     gene            7547..8059
                     /locus_tag="O2701_000674"
     CDS             7547..8059
                     /locus_tag="O2701_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HCZ4292580.1"
                     /translation="MEKGNQGIKWSSLIMGVLLLMLAVVIFTFPIENFYAITWLIGLF
                     VLINGVIQIVYRRKAKALVGGNQNWILFMGIVDILFGLLVIFNVGASSAFFIYMFAFW
                     FIFSSISGLFTFSGSGSLKLISVIFNLLGIVFGVILLFNPLMGIVFISTMIAIAFVFV
                     GVIYVVDALA"
     gene            complement(8193..8987)
                     /locus_tag="O2701_000675"
     CDS             complement(8193..8987)
                     /locus_tag="O2701_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HCZ4292581.1"
                     /translation="MNIAKLENYLQIDSSRYNRPSIEALNYYATRFMLTVPFENIDVQ
                     NSKPISINIDALFNKIVHDKRGGFCYELNTFFKAYLQQKGFNPELMSATIHTPGGGRS
                     LNGSHASLVVSINDVFYVTDVGFGDLPLHAIPITSSEHTQPITDISGTFRAIFNNEDK
                     DIFYVQKFENDHWHTKYEAEFKPKQIEDFNSNIEYNQTHPDSIFVQHLLITMPQSFGR
                     ATMSENHLTLTRNGSSEKFDVTKDNYKHFLEKYFGLNVTINRIEKQ"
     gene            complement(9230..10246)
                     /locus_tag="O2701_000676"
     CDS             complement(9230..10246)
                     /locus_tag="O2701_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HCZ4292582.1"
                     /translation="MTVFKNERLSWLPYIAIVILLHVIGFSFLWIAGKDHHILFGMGI
                     LAYTLGLRHAFDADHIAAIDNTVRKLLQQRKDPSGVGFYFSIGHSSVVFLMAVFLGVS
                     VKWAKDELPHFQDIGGTIGTLVSGFFLVLIGVLNLIILISLINLFAKLRREHIEEAEV
                     DALLESRGLVSRFVGPYFKLITRSWHVLPLGFLFGLGFDTASEIALLALSSGASQQAI
                     SFIGILSLPILFASGMSLLDTLDGVVMKYAYNWAFFNPIRKIYYNITITAISVMAALV
                     IGMIELLQILADKLDLHGAFWAFIGSIEFDYLGYILVALFLITWLISSLIWKFGRIEH
                     KWSR"
     gene            complement(10411..10986)
                     /locus_tag="O2701_000677"
     CDS             complement(10411..10986)
                     /locus_tag="O2701_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCZ4292583.1"
                     /translation="MTLPKIGKPATRALNSQGIYTLEAVSQYTKSSLMEMHGVGPKAI
                     SILEQALFQHQLHFKTEVQSSLPFKLTGDVSCNHAPKRQQMIDFIVVTAALDIELLRS
                     LVTTEFIWSVPGRFDIYGPQILIQELSNHYNQVASLNIHSSITHGCLGSMHGIEILKT
                     GKEIHFAHFFEFENHKKDAKLSKVTSYIVVG"
     gene            11211..12119
                     /gene="rarD"
                     /locus_tag="O2701_000678"
     CDS             11211..12119
                     /gene="rarD"
                     /locus_tag="O2701_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCZ4292584.1"
                     /translation="MNSEYKKGIFLALSAYILWGILPIYWQFVDAIGAFEILAFRIIF
                     SAIFMIFILAVGQKQRNAFQRDMNQLLGKPIQLLAIVVAGYVITLNWGTFIWAVTNGH
                     VLQTSLGYYINPLVSILLALIFLKERFNKFEWLAILFAFIGVLYMTLKIGEFPIVSII
                     LALSFGTYGLLKKVVHIDAISSITIECIVTAPAGLIYVIYLWQQHQMSFGLNMSSFWL
                     LFSGAITAIPLILFSAGAKRIPLSLIGFIQYVGPTIMFVLGIFVFKEPFSIDQLITFI
                     FIWTGIVLYSLSQYIKLKKHPVAKTL"
     gene            complement(12532..13950)
                     /locus_tag="O2701_000679"
     CDS             complement(12532..13950)
                     /locus_tag="O2701_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCZ4292585.1"
                     /translation="MENTVKYRKFILPIVVGLLIWALTPFKPDAVDPTAWYMFAIFVA
                     TIIACITQPMPIGAVSIIGFTIMVLVGIVDMKTAVAGFGNNSIWLIAMAFFISRGFVK
                     TGLGRRIALHFVKLFGKKTLGLAYSIVGVDLILAPATPSNTARAGGIMFPIIKSLSES
                     FGSKPKDGSARKMGAFLVFTEFQGNLITAAMFLTAMAGNPLAQNLASSTSNVHITWMN
                     WFLAALVPGLVSLIVVPFIIYKIYPPTVKETPNAKSWAENELATMGKIALAEKFMIGI
                     FVVALTLWIVGSFIHIDATLTAFIALALLLLTGVLTWQDILNETGAWNTLVWFSVLVL
                     MADQLNKLGFIPWLSKSIATSLGGLSWPIVLVILILFYFYSHYLFASSTAHISAMYAA
                     LLGVAIAAGAPPLFSALMLGFFGNLLASTTHYSSGPAPILFSSGYVTQKRWWTMNLIL
                     GFVYFIIWIGLGSLWMKVIGIF"
     gene            complement(14188..14652)
                     /gene="bstA"
                     /locus_tag="O2701_000680"
     CDS             complement(14188..14652)
                     /gene="bstA"
                     /locus_tag="O2701_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol transferase BstA"
                     /protein_id="HCZ4292586.1"
                     /translation="MTTKTVFDVIDMGLGYLVNVYDAWKVEKVLDDYHKPFSNTIHWQ
                     FGHVLTIFESALAVAGKENIDLNIYRPLFGNGSSPDEWKDEVPSIERILEGLQTLPER
                     ARNLTEDDLAIELKQPIVGCNNLEELLVLNAIHIPLHAGKIEEMSRILKNLK"
     gene            14819..15157
                     /locus_tag="O2701_000681"
     CDS             14819..15157
                     /locus_tag="O2701_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCZ4292587.1"
                     /translation="MSVSDQIMKGLLDGAILGLIAQGETYGYEIMTKLKNQEFPEISE
                     GSIYPVLMRLNKKGYVDTTIKKSSNGGPRRKYYTITDSGLKELKVFKSKWQVLNKGMI
                     NLFGGENDVK"
     gene            15147..15914
                     /locus_tag="O2701_000682"
     CDS             15147..15914
                     /locus_tag="O2701_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292588.1"
                     /translation="MLSKQSQDFLEKLRVELLFRGKTEAEVEELLEELEDHLTMAEKN
                     NEDTSSIINTPIKSMADQLSPEISLTTGLYKYITLYFAFILAVIIIPRFLDLGTFDVT
                     IAFLLYIASIVILGVVIGMLILRNTLVRFGDQKITYVINVTYGIIVFLWMILGGLIIK
                     KYPIYSFFELSDKQSFIVGLVLLIALIGTCILIKQKIYALIVLVISLPSLIARIAAIF
                     SKEPDTFNLVSVSILIILNIAFIIYTFVNFRKLKKED"
     gene            complement(16033..16671)
                     /gene="pcp"
                     /locus_tag="O2701_000683"
     CDS             complement(16033..16671)
                     /gene="pcp"
                     /locus_tag="O2701_000683"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HCZ4292589.1"
                     /translation="MHILVTGFAPFDNQNINPSWEAVTQLEDIIGTHTIDKLKLPTSF
                     KKVDNIINKTLASNHYDVVLAIGQAGGRNAITPERVAINIDDARIPDNDDFQPIDQAI
                     HLDGAPAYFSNLPVKAMTQSIINQGLPGALSNSAGTFVCNHTLYHLGYLQDKHYPHLR
                     FGFIHVPYIPEQVIGKPDTPSMPLEKIVAGLTAAIEAISNDEDLHLALGTTE"
     gene            complement(16823..17779)
                     /locus_tag="O2701_000684"
     CDS             complement(16823..17779)
                     /locus_tag="O2701_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCZ4292590.1"
                     /translation="MNYQVLLYYKYMTIDDPEQFAQDHLAFCKAHHLKGRILVSTEGI
                     NGTLSGTKEETEQYMAHMHADERFKDMVFKIDEAEGHAFKKMHVRPRKEIVALDLEDD
                     VDPRHTTGQYLSPVEFRKALEDDDTVIIDARNDYEFDLGHFRGAIRPNITRFRDLPDW
                     IKENKALFADKKVVTYCTGGIRCEKFSGWLLKEGFEDVAQLHGGIATYGKDPETKGEY
                     WDGKMYVFDDRISVDINQVEKTIIGKDWFDGKPCERYINCANPECNKQILVSEENETK
                     YLGACSYECAKHERNRYVQANNISDNEWQQRLTNFDDLHQHA"
     gene            18023..18994
                     /locus_tag="O2701_000685"
     CDS             18023..18994
                     /locus_tag="O2701_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HCZ4292591.1"
                     /translation="MSQQDLPTLFYSGKSNSAVPIISESELQTITAEPWLEISKKGLQ
                     LEGLNFDRQGQLFLLDVFEGNIFKINPETKEIKRPFVSHKANPAAIKIHKDGRLFVCY
                     LGDFKSTGGIFAATENGDNLQDIIEDLSTAYCIDDMVFDSKGGFYFTDFRGYSTNPLG
                     GVYYVSPDFRTVTPIIQNISVANGIALSTDEKVLWVTETTANRLHRIALEDDGVTIQP
                     FGATIPYYFTGHEGPDSCCIDSDDNLYVAMYGQGRVLVFNKRGYPIGQILIPGRDEGH
                     MLRSTHPQFIPGTNQLIICSNDIEMGGGSMLYTVNGFAKGHQSFQFQ"
     gene            complement(19143..19658)
                     /locus_tag="O2701_000686"
     CDS             complement(19143..19658)
                     /locus_tag="O2701_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HCZ4292592.1"
                     /translation="MTNFTFDGAHSSLEFQIKHLMVSKVKGSFDQFDVAVEGDINDFS
                     TLKATATIIPSSINTKNEARDNHLKSGDFFGTDEFDKITFVTKSVSESKVVGDLTIKG
                     ITNEETFDVEFNGVSKNPMDGSQVTGIIVTGTINREKYGINFNQALETGGVMLGKDVK
                     FEASAEFSISE"
     gene            20038..20886
                     /locus_tag="O2701_000687"
     CDS             20038..20886
                     /locus_tag="O2701_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HCZ4292593.1"
                     /translation="MGNYLVLQSDFGLSDGAVSAMYGVAYTVSDDIRVENLTHDIPPY
                     DIWVASYRLYQTVKYWPKGTVFVSVVDPGVGSDRRSIACLTYSGHYIITPDNGSLSHI
                     KHYEGIKKVIEIDEVKSRLPHSEESHTFHGRDVYAYNGARLAANKIAFERLGQAINVD
                     SIEALEIREVTKNEDSVTGSIDILDIRFGSLWTNIPLAFLKSLEIVHGNNLVVTIYHQ
                     DKKVYQNIIKFARSFADVNVGEPLVYVNSLVNIGVAVNQDSFSDLYHIGTGNDWTIHL
                     SKAPSI"
     gene            20915..21469
                     /locus_tag="O2701_000688"
     CDS             20915..21469
                     /locus_tag="O2701_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HCZ4292594.1"
                     /translation="MKKQDISVKTVVAIGIGAAVFVILGRFVVIPTGFPNTNIETSYA
                     FLALISAIFGPFAGLMTGLVGHAIKDFTTYGSAWWSWVICSGIIGCLYGWIGLKLNLS
                     SGRFSRKSMVYFNIGQIIANIICWALIAPTLDILIYNEPANKVYTQGVISAVLNIISV
                     GIIGTILLKAYASSQIKKGSLRKE"
     gene            21522..23234
                     /locus_tag="O2701_000689"
     CDS             21522..23234
                     /locus_tag="O2701_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4292595.1"
                     /translation="MTEPIISFKDFSFQYHSQATPTLQNINVDIYPGEKVLVVGASGS
                     GKSTFANCINGLIPFKTKGNITGELYINNQDATVSCLHDRSNVVGTVLKDTDGQFIGL
                     TAAEDMAFLLENNCVEQDDMKKNVSYWAEKVGMIEHLNHRPQDLSGGQKQRVSLGGIL
                     IHRTPILILDEPLANLDPATGHETLRLLNNIHEETKSTMIIVEHRLEESLDDTFDRVL
                     LFKDGKIIANTTPSDLLKSSKLKEAGIREPLYCTALKYAEVDVESIDNLANLRDVCMS
                     EHVKFKVKKWIDETSANNDNKYKSEPLLELNEVCVQYSDYSNSVLNNVQLNVYRREML
                     SIVGHNGAGKSTLAKAICGFLDITGNIQFCNRGFNQLSISERSEFVGYVMQNPNHMIS
                     EKMIYDEVALGLRARGMKESDIKIRVENVLKICGLYAFRNWPIAALSYGQKKRVTIAS
                     VLVLNPEIIILDEPTAGQDFYHYNEIMSFLIELNRQGKTIIMITHDMHLLSEYSSRTV
                     VLSKGQVVADTTPVLVLNDKKICEIASLRQTSLFEMAEYIGISEPQKLVQLFINHDRK
                     VRRQ"
     gene            23231..24064
                     /locus_tag="O2701_000690"
     CDS             23231..24064
                     /locus_tag="O2701_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCZ4292596.1"
                     /translation="MNQYNTIGFHPGNSRIHQLNATVKLLFLLVVSISAMVTYDTRYL
                     ILISASSILLVKYAHIEWKQVRFVVKFILFFTILNIIAVYIFDPEYGVKIYNQRTELV
                     NGIGRFTLTSQELFYLFNLILKYISTVPLALIFLFTTNPSHFAASLNQLGVNYKISYA
                     VSLALRYIPDIQETYFNISQAQQARGYDNSKKAKFTSRVKGIKRIVLPLIFSSIERID
                     TISTAMELRQFGQYKRRTWYVKKQLKKDDYVVLCLTLILLMLVVTLFFLNNSRYFNPW
                     H"
     gene            24111..24608
                     /locus_tag="O2701_000691"
     CDS             24111..24608
                     /locus_tag="O2701_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4292597.1"
                     /translation="MINTERLNLMIPSSSHLIELYNICSHPQANIYTPKGLHNSKLDT
                     QRWIEKWRNHWQQYQFGYFVLVKKIDCSVIGICGYEYRQLKQETVLNLFYKLHPSFEG
                     QGYACEAITAITNFVNYIDQETVKVIRTNKCNQRSINLAERLKFKRDDTMDDIINQGD
                     IVFYK"
     gene            24726..25850
                     /locus_tag="O2701_000692"
     CDS             24726..25850
                     /locus_tag="O2701_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCZ4292598.1"
                     /translation="MHKKYLLLFFLLLSVALLYSCSKQNEKAQNKSVNPNEYAKHKSK
                     MDEHWETYHGQVYHVFYHPLITDPKVAFTGDTHQAKGNNDWMITVSEFKKSLNELYKN
                     NYIIVNPHDVYDLSSHHVKAKKIKLPKGKKPLIISIDDMNYYSYMRHHGYADRLVLDK
                     NKHVVSETTDKKGHATYSDDNDIVPILNKFVKEHPDFSLNGEKGVVALTGYEGVLGYR
                     TNELTSKDYTKNKKAAEEVVRAMKRDGWSFASHSYGHINFEKTSLEGIKRDTKRWKDE
                     VEPIVGKTDMFVFPHGAQDRHTQAYDYLVDEAEFKFIAGVGPNNFTDISATNVYQDRV
                     AIDGLNLFEFKYKLKPFFNPENVYSKQDRRYFKGNRDYEE"
     gene            26155..26973
                     /locus_tag="O2701_000693"
     CDS             26155..26973
                     /locus_tag="O2701_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HCZ4292599.1"
                     /translation="MNNSEQLIALKESETAFLKYFNKADYELVDFSVVEKLDWKQLNH
                     EDLQQMGERNFWQHEHQIYALRNDFTDQLLRYYSMYPTAATKVAYTGLIIRNNEAAVQ
                     VGLENYAPSLANVQQSLKLFIQFIQQQLRDNVHFVVLGHYQLLDALLDKSLQTPDILS
                     MIEERNLSGLVTYLSTEHPIVQILKENTQQQLNVLEHYIPNDHPALVELKIWERWLHK
                     QGYKDIHLDITAQPPRSYYTGLFIQCHFAENESRVLTGGYYKGSIEGFGLGLTL"
     gene            26991..27605
                     /locus_tag="O2701_000694"
     CDS             26991..27605
                     /locus_tag="O2701_000694"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCZ4292600.1"
                     /translation="MLRIAIAKGRLMDSLINYLDVIEYTTLSETLKNRERQLLLSVDN
                     IECILVKGSDVPIYVEQGMADIGIVGSDILDERQYNVNNLLNMPFGACHFAVAAKPET
                     TNYRKIATSYVHTAETYFKSKGIDVELIKLNGSVELAGVVDMVDGIVDIVQTGTTLKA
                     NGLVEKQHISDINARLITNKAAYFKKSQLIEQFIRSLEVSIANA"
     gene            27598..28848
                     /locus_tag="O2701_000695"
     CDS             27598..28848
                     /locus_tag="O2701_000695"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCZ4292601.1"
                     /translation="MLNAQQFLNQFSLEAPLDESLYPIIRDICQEVKVHGDKALKMYN
                     LTFDHTKTDHLEISHEQIKAAFDTLDEKTKQALQQSYERIKAYQESIKQTNQQLEESV
                     ECYEIYHPLESVGIYVPGGKASYPSTVLMTATLAQVAGVENIVVVTPPQPNGVSQEVL
                     AACYITQVDQVFQVGGAQSIAALTYGTETIPKVDKIVGPGNQFVAYAKKYLFGQVGID
                     QIAGPTEIALIIDETADLDAIVYDVFAQAEHDELARTYAISEDAQVLKDLESRIAKAL
                     PNVDRYDIVSKSIANQHYLIHASNFDEACHVMNTIAPEHASIQTVNPQPYIEKVKYVG
                     ALFIGHYSPEVIGDYVAGPSHVLPTNRTARFTNGLSVNDFLTRNTVIHLSKDTFEQIA
                     DSAQHIAHVEALYNHQQSILIRQS"
     gene            28864..29877
                     /locus_tag="O2701_000696"
     CDS             28864..29877
                     /locus_tag="O2701_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HCZ4292602.1"
                     /translation="MIYIDKNESPVTPLDEKTMTSIISATPYNLYPDAAYEQFKEAYA
                     KFYGLSPEQIIAGNGSDELIQKLMLIMPEGPALTLNPDFFMYQAYAAQVNREIAFVDA
                     GSDLTFDLETILTKIDEVQPSFFIMSNPHNPSGKQFDTAFLTAIADKMKALNGYFVID
                     EAYLDYGTAYDVELAPHILRMRTLSKAFGIAGLRLGVLISTAGTIKHIQKIEHPYPLN
                     VFTLNIATYIFRHREETRQFLTMQRQLAEQLKQIFDTHVADKMSVFPSNANFVLTKGS
                     AAQQLGQYVYEQGFKPRFYDEPVMKGYVRYSIATASQLKQLEEIVKEWSAKYDLSKTT
                     KHS"
     gene            29846..30424
                     /gene="hisB"
                     /locus_tag="O2701_000697"
     CDS             29846..30424
                     /gene="hisB"
                     /locus_tag="O2701_000697"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HCZ4292603.1"
                     /translation="MIYQKQRNTAETQLNISISDDQSPSHINTGVGFLNHMLTLFTFH
                     SGLSLNIEAQGDIDVDDHHVTEDIGIVIGQLLLEMIKDKKHFVRYGTMYIPMDETLAR
                     VVVDISGRPYLSFNASLSKEKVGTFDTELVEEFFRAVVINARLTTHIDLIRGGNTHHE
                     IEAIFKAFSRALGIALTATDDQRVPSSKGVIE"
     gene            30421..30999
                     /gene="hisH"
                     /locus_tag="O2701_000698"
     CDS             30421..30999
                     /gene="hisH"
                     /locus_tag="O2701_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCZ4292604.1"
                     /translation="MIVIVDYGLGNISNVKRAIEHLGYEVVVSNTSKIIDQAETIILP
                     GVGHFKDAMSEIKRLNLNAILAKNTDKKMIGICLGMQLMYEHSDEGDASGLGFIPGNI
                     SRIQTEYPVPHLGWNNLVSKHPMLNQDVYFVHSYQAPMSENVIAYAQYGADIPAIVQF
                     NNYIGIQFHPEKSGTYGLQILRQAIQGGFIND"
     gene            30992..31696
                     /locus_tag="O2701_000699"
     CDS             30992..31696
                     /locus_tag="O2701_000699"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCZ4292605.1"
                     /translation="MIELWPAIDLIGSTSVRLTEGKYDSEEKMSRSAEESIAYYSQFE
                     CVNRIHIVDLIGAKAQHAREFDYIKSLRRLTTKDIEVGGGIRTKSQIMDYFAAGINYC
                     IVGTKGIQDTDWLKEMAHTFPGRIYLSVDAYGEDIKVNGWEEDTELNLFSFVRRLSDI
                     PLGGIIYTDIAKDGKMSGPNFELTGQLVKATTIPVIASGGIRHQQDIQRLASLNVHAA
                     IIGKAAHQASFWEGLK"
     gene            31693..32451
                     /gene="hisF"
                     /locus_tag="O2701_000700"
     CDS             31693..32451
                     /gene="hisF"
                     /locus_tag="O2701_000700"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCZ4292606.1"
                     /translation="MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAMYYNEAGA
                     DELVFLDISKTEEGHSLMLEVIEQTASRLFIPLTVGGGIQSLDDITQLLNHGADKVSL
                     NSSALKNPQLIKQASDKFGRQCICIAIDSYYDPERKAHYCCTTGGKKMTNIKVYDWVQ
                     QVEQLGAGELLVTSMGHDGMKQGFDIEHLANIKSLVNIPIIASGGGGNAQHFVELFDQ
                     TDVSAGLAASILHDRETTVQSIKEVIRQGGIAVR"
     gene            32448..33080
                     /locus_tag="O2701_000701"
     CDS             32448..33080
                     /locus_tag="O2701_000701"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCZ4292607.1"
                     /translation="MTKYKIDFSKGLVPAILQDNQTKQVLMLGYMNQEAFDKTIEDGV
                     VCFYSRSKQRLWTKGETSGHTQRVKDIHVDCDNDTILIDVIPNGPTCHTGSQSCFNTE
                     VPFSVQTLAQTVQDSAQSNNEKSYTKYLLTEGIEKITKKYGEEAFEVVIEAIKGDKKA
                     FVSEVADELYHLFVLMHALGVDFSEIEAELARRHHKRNNFKGERQNIEQW"
     gene            33273..33452
                     /locus_tag="O2701_000702"
     CDS             33273..33452
                     /locus_tag="O2701_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292608.1"
                     /translation="MKSKLSNSGIDVTVKDVEKYMNRYNEVMKGKNGEKAKELCLSLL
                     PINIIVVFTFFVFIL"
BASE COUNT        11661 a         5114 c         6114 g        11010 t
ORIGIN      
        1 ccttgctggg gtgggacgac gaaataaatt ttgcgaaaat atcatttctg tcccactccc
       61 taatttgagc tggatatact ttcatttgaa ccctttattg ctagtttatg aaagtatcat
      121 gaaagcttta tgaacatcgc ttgagttgcc tttacagtag aaaatttaag ttttacactt
      181 tgtgtgaatg atacgttttg tattgaatta attatagaaa ggtacgttga agatgttttc
      241 aattggaagt gcaattcttc attttgtcat tggtggtatc gctgttgcat tagcttcaat
      301 tattgctgat aaggtaggtg gtaagttagg aggtattata gctactatgc cggcagtctt
      361 tcttgcggct attatcgcat tagctttaga tcatcgtggt acgcaattag tggagatgtc
      421 gatgaatctt agtactggag caattgtcgg tattctgtct tgtatattaa ctgtattttt
      481 gacatctctc tacattaagc ataaaggtta tcggaaaggc gcaatattca cagttgtttg
      541 ttggtttgtc atttccctcg caatattcag tattagacat ttatagtttg gaaaatgcgt
      601 gataattagt tgtattcagt tattaagtaa taaattattg gaggcagaac atcatgaaat
      661 taacattaat gaaatttttt gtggggggat ttgcagtatt attaagttat attgtatctg
      721 taacactacc ttggaaagaa tttggcggta tatttgcaac gtttccggca gtatttttag
      781 tgtctatgtt tattacaggt atgcaatatg gtgataaagt cgctgtgcat gtaagtcgtg
      841 gcgcagtgtt tggtatgaca ggggtattag tttgtatttt agttacatgg atgatgttac
      901 atatgacgca catgtggttg attagcattg ttgttggttt cctaagctgg ttcatcagtg
      961 cagtatgtat ttttgaagcg gtagaattta tagcacaaaa aagattagaa aagcatagtt
     1021 ggaaagctgg aaaatcgaat agtaaatagt gtgaacgtaa tctcttaact aggactaact
     1081 ttgcaagcat tgaatagcat ggaaaagttg catcattaat aagtgaaatt caagttggca
     1141 ttgagaaaat tacaagcgcg taatcataca ggtctgtctt aagggagtct tcgaaccccg
     1201 atgttgtcgt atgtcaaaac atttagtcaa tcataaaggt gacttgattt aactttatct
     1261 gatagtctga ttgtaatgat tgtactaatt gactggaggc gtatgtaatt gaatctgagt
     1321 aaacaaatta aaaagtatag ggaacgagat ggttattcac aagaatatct tgctgaaaag
     1381 ttatatgtat ctaggcagag tatttctaat tgggaaaatg acaaaagctt accagacata
     1441 cataacttat taatgatgtg tgaattgttc aatgtaactt tagatgattt agtaaaaggg
     1501 accattccat ttgtacctga tattaaagcg caacgaagtc ttaacttatg gacatatgtg
     1561 atgcttattt tcatgacatt agctgcaatt ttaatgggac ctttagttgt ttattggaat
     1621 tggacttggg gtgtaacggt ggcaatcatt ttgggaatag gtttttatgc atctatgaaa
     1681 atagaagatt taaaaaaagt gcataaaatg gacaactacg atcgaattgt tgcttttatg
     1741 aatggaaaag atcctagtga agtacaaacg actaaagcta gaaatacgat gacaaatgcg
     1801 ctttctatta tatcagtaat tggtatactc agcctcataa ttttccttag tgtgtatttg
     1861 gcaaataagt ttttataaat catcgtggta tcgtctcata ttatttatat tatccaaaat
     1921 agcataaaaa aataccaaca agatttagaa ccttgttggt aatcaaagcg attcatttat
     1981 aatgagtcgt tttatgttgt aagattaaac agtttgtacg ttaactgctt ggtctccacg
     2041 ttgaccttca gtgatttcga aagtaacttt ttgaccttct tctaaagttt tgtagccatc
     2101 gctagcgata cctgagaaat gtacgaatac gtctccgcca ttttcttgtt cgatgaaacc
     2161 aaaacctttt tctgcgttaa accattttac tgtaccgtta ttcatataga aaacctccgt
     2221 gtgcttttgc acttaatatt tgtaacaaat tcataactaa aaaagaggat attctaaaca
     2281 aatacactac aatttaattc acgagctttt attacgtaag accaactata cgctcatatt
     2341 ggcataatgt acagtgtttt ttgaaaataa attaaaaaag atttttaaaa accttagaaa
     2401 cgttgattta aaggggttta taaaaataaa aaaattgtag tcttttatgg tgtttgctag
     2461 ttttcaaagt gacatatcgt ttaaacatga tgattttata agcaatccat aaaaaacaag
     2521 cagcgataaa cgctacttgt tgatattaaa atctgacttg aaaggtcata gcaatgttct
     2581 ataccgatgg aatgtgctta cttgcctttt tcttcacgac gttttaaata ataagagcca
     2641 cctaataaac cagctggaat gcctatcatt ggtgttgtga atgagcttaa tacaataaca
     2701 agtattgtta aagcaatgac gttataccaa gttacagtca aatttttcaa atcctcatat
     2761 gattgtttta ctaattctct aaatttcatg attcaatctc tcctttttta taaatcttta
     2821 gattgtcaaa ttaagctgga caagtttatc atcttcgatg aaactcataa atacttccaa
     2881 tggtgttcta taacctaaag attttctagg atgatgattg attttttgag caacactgat
     2941 gatatgatca tccgttacta aattgaaatc tagttcttta tctaatccac tgcgtctaat
     3001 tattccgtta gaatgttcat ttaaacctcg ctgtgatggc gtgcctggat cagcaaaaaa
     3061 tatagatata tcgtgacagt tacatattgt tttccagttt gagaactcct taccacaatc
     3121 aaaagtaatt gatttaaata tatgagaaga taaaatgaaa aggtgcaagc agtattaaga
     3181 atttaggtag caagaaaaca agaagtggta catacgatgc taacctaaaa agaattggag
     3241 attaaaatga ttattaacct tgaaacacaa caaatacatt ttgatttaga acatgaaatt
     3301 ccctattttc aagcacctga gaaaaatagg atacgcttag atattgatgt tctcaataaa
     3361 aagcaaattt ctaatattat taatgttata tttaataacc aatcaaagac gaaatgtaca
     3421 tttttgtctg aatacttata tcctgttaaa tttagagaaa aaacaagaat tggtcgtttt
     3481 tttaatatta ctaactggta cgaagaaatc cattctaccg atgagaagta tgttattgca
     3541 actattaaaa atataaaaaa caaagactta attgattatt ctttatatat taaaaaaggt
     3601 ttagtggagc catttattgt attctataat caagaaaata tattctacat tagcaatgct
     3661 gttatagata tcatcagtgt atccaaagat actcttgatt atataaagtc agaattcaat
     3721 gatcactgtg cttcgccata ctgattttat agcacagaga aaagccgtag tttcgaagag
     3781 accaacggct tttttataaa tctttagatt gtcaaattaa gctggacaag tttatcatct
     3841 tcgatgaaac tcataaatac ttccaatggt gttctataac ctaaagattt tctaggacga
     3901 tgattgattt tttgagcaac actgatgata tgatcgtctg ttactaaatt gaaatctagt
     3961 tctttatcta atccactgcg tctaagtatt ccgttagaat gttcatttaa acctcgctgt
     4021 gatggcgtgc ctggatcagc aaaaaatata gatatatcgt gacagttaca taatgttttc
     4081 cagtttgaga actccttacc acaatcaaaa gtaattgatt taaatatatg agaaggtaaa
     4141 gatgaaaaca ttgagtgtag agcgttttct atatcagttg ccttacgacc taagggtcgt
     4201 aaggtgataa tcatcttgga tactctttct accaaagtaa tgattgcact accatgacga
     4261 cgaccaacaa tagtatctcc ttcaagatgt ccaaactcct tatgttttct ttggtaggaa
     4321 atacatattg agtttagcac ataaggctta tttatttacc tttttcttca cgacgtttta
     4381 aataataggc gccacctaat aaaccagctg gaatgcctat cattggtgtt gtgaatgagc
     4441 ttaatacaat aacaagtatt gttaaagcaa tgacgttata ccaagttaca gtcaaatttt
     4501 tcaaatcctc atatgattgt tttactaatt ctctaaattt catgattcaa tctctccttt
     4561 tcttaaatca attaatagtt atactgcatt gcttaaaatc aaaatatcta atgaagcatc
     4621 ttttaatcgt aagtgttaaa tggttttctt aatcaatttg tttgaatgta caaaagcaat
     4681 taaagcaaaa gtaatgtaaa tgagggtgta tacaacaatt actacaataa ccggcatgaa
     4741 ggaaatatta cccatcaatt tcataaatgc tattgcggcg aatactgcat gtaatatagc
     4801 aatcaataat ggtaaaccga agttgaaagt aatttttaat aataatccct taagcatatc
     4861 tgtatgcgta aagcctatgc gttttaatat tctgaagtta cttagttcat cctcagtttc
     4921 atccatttgt ttaatataaa taatacatcc agctgctact aaaaatgcta atcctaaaaa
     4981 tgatgtaaca aatattagaa taccgttagt agcatcgacc tcttttttca tttcatcata
     5041 cgtgatgact ttgtctccaa actgttttgc aattgcttga gctttttcct tttgtgatgt
     5101 ttgtttaata tcatatccat aaaaagtatg aacgttattt tgtgttttca actgctgata
     5161 cttttcagga cttacttcga tgacaggtga gttgaagctt agatttaaag gataaacctt
     5221 acctttgtct tcttgtgtaa cacggaaagt ttcattctta gtacctttta ctactaaatc
     5281 tttgtttaaa tggatattaa taatgttagg cagcgatttt gtatttgtaa tgatggcatt
     5341 gttgcctgtt aacttgttat ttgcacttaa aatagaattc gtgcgacctg aatcactacc
     5401 attttccaaa gtaataacct gatcattaac attatctaca gtaatagttt cgtaagcatt
     5461 tttagaaaat gtaatttgtt gttggcttag tttagtttca aattgtttag catcttgagt
     5521 agcgaccacg ttaaattcat ttggtgccat agatgtaagg gtttgatctg tatttgattt
     5581 agataatgcc gcaaaacaca atacagttac tgtaactgca gaaataattg caatgatagt
     5641 taaagacatg gcatttttct tcattctgta cataatagac gatgtgaata caacatcggt
     5701 aatagatacg cgtccatttt ttgatttttt caatgtttta aaaataagtg acacggaact
     5761 tctgaagaat aaataggcgc ctacaaccgt taaaaataaa atgataaacg gtgatgtcat
     5821 agccatagtt agtgctttga acgtaccaaa catttctgtc gccatataat agcctagtgc
     5881 aatcatagca atacctaata cgcctgaaat aacctctgca gtcgttactt tagcagttgt
     5941 ggcatcagtt ttaattgaat ctttcatcat tgataagata ctacgtcttt ttagaaataa
     6001 agcactttta aataaaatca gtacatacgc aataattagc atgaaaatag ttaaaacaag
     6061 ggccataggt tcgaaatgta tcgataagtt aatcgataac gacatcaatt tagatactat
     6121 ggaaagcaat aattgtgcac ctgcaatgcc acataataca ccgacaacac ctgtgattaa
     6181 aaatacgatc atttgttcaa gtgctaacat tttcaaaatg ttttgtcgtg ttaaaccaat
     6241 caactgaaat agcgcaaatt cacgtgtacg gcgttttacg aataaatgat tggcatacat
     6301 taaaaagatg acaataatga taaataaaaa tattgatccg actaaagcac ctttcttaat
     6361 gatggccatc gagtcgtcat tatttacacc tttagtaaac tgtaaggttg taaaactgaa
     6421 atataagacg atgctaaaaa ataatgaaaa taaatacatt gcataatgtt ttaagttttg
     6481 tcgtaagttt ttgaaaacga tatggttaaa tgtcatttga gacaccacct aatactgatt
     6541 gaagatgtac aatgtcttca taaaaggcct gtttagaacg tccttcctga taaagttgtg
     6601 tatgaatttg gccatctttt agcatgatga cacgttctgc aaaacttgct gcaaccggat
     6661 catgtgtaac catgacaata gttgtatcaa acgatttatt catttcttcc aaacgttgta
     6721 ataggtcatt tgcacttttc gagtcgagtg cgcctgttgg ctcatctgca aatatgattt
     6781 gtggtttgtg aacaaatgct ctcgctgctg cagttctttg ttgttgacca ccagataatt
     6841 cgctagggta tttatttcct aggtcataaa tacctaatgc tgtcgtgatc gctttataat
     6901 tttcttccat tgttgccttc gacatttttt gaacagataa aggtaacata atgttttctt
     6961 taacggttaa tgtcggcaga atactgtaat cttggaagat gaaacctaat gattctttgc
     7021 ggaatttggc aagtgctttt tgattaagtt tattaagctc ttgtccgtta gcaatcacgc
     7081 taccgctaga aatttggtca attgaactta gtacatttaa taaggttgtc ttacctgatc
     7141 cagaaggccc cataatcgca acgaattcgc ctttttgtat gtcaaagtta atatctttaa
     7201 gtgcttgaaa tgtgtgcttt ttaccgtatg tttttgaaac atgttgcact gataatatcg
     7261 tcatagtctc actccttttg tatttaattt cattttaaat aatgtttgga gtagtagcct
     7321 ttatctaaac ttacaattca atgaatgaac cttacagagt tgaaagctat cgctacttag
     7381 tagatttttg agtgaggata cagattcatc gtacatatta gacaaaagca atggtgcttt
     7441 ctaagtgatg atgtttgtgt aaattgagaa aagggaattt aattattgta taataaattt
     7501 tttgtaaaaa ttaaaagagg gttttatttg aaaggaattg attgttatgg aaaaaggaaa
     7561 tcaaggtatt aaatggtcta gtttaataat gggtgtatta ttattaatgt tggcagtcgt
     7621 tatttttaca tttccaattg aaaattttta tgctattacc tggttgattg gactgtttgt
     7681 attaattaac ggtgtgattc aaatcgttta ccgtagaaaa gcaaaagctt tagtaggtgg
     7741 taaccaaaat tggattctgt ttatggggat tgtagatatt ctatttggtc tattagttat
     7801 ttttaatgtt ggcgcaagtt cagcattctt tatttatatg tttgcttttt ggtttatttt
     7861 tagttctatc tctggattat ttacgttttc gggtagtggt agcttaaaac taatttcagt
     7921 gatttttaat ttattaggta ttgttttcgg tgtcatttta ttatttaatc cattaatggg
     7981 tatcgtcttt atttcgacga tgattgctat tgcatttgta ttcgtaggtg tcatttatgt
     8041 tgtagatgca cttgcttaag taaaatgaag cggttcaaaa gaagggtgtg acatgaagtt
     8101 tgtgtcatat cctttttgtt gtgtttatga agcataaaaa aggggcgcta cctacaataa
     8161 gtaagatacg cccatatttt tatattttac tattattgtt tttcaatacg attaatagtt
     8221 acatttagtc caaaatattt ttctaaaaaa tgtttatagt tatctttagt gacatcaaat
     8281 ttttctgagc taccattcct tgttaaagtt aaatgatttt cagacattgt agcacggcca
     8341 aatgattgtg gcattgtaat taataaatgc tgtacaaata ttgaatctgg atgcgtttga
     8401 ttatattcga tgttgctatt aaaatcttct atttgtttag gtttgaattc agcttcatat
     8461 tttgtatgcc aatgatcatt ttcgaatttt tgaacataga aaatatcctt gtcttcgttg
     8521 ttaaaaatag cgcggaatgt accactgata tcagtaattg gttgtgtatg ctcagatgaa
     8581 gtaataggaa tggcatgtag aggtaagtct ccaaagccaa catcagttac atagaataca
     8641 tcatttatag aaacaacaag tgaagcatgt gaaccgttca gactacgacc gccaccgggt
     8701 gtgtgaatag tagctgacat taattcagga ttaaatcctt tttgttgtaa ataggctttg
     8761 aaaaatgtat ttaattcata acaaaaacca ccacgtttat catgaacaat tttattaaaa
     8821 agtgcatcga tatttataga tatcggctta ctattttgaa catcaatatt ttcaaaaggt
     8881 acagttaaca taaaacgtgt tgcataataa tttaatgctt caatactcgg tcgattataa
     8941 cgagatgaat caatttgtaa ataattctct aacttcgcaa tattcataag catagcgcct
     9001 cctgtattaa agattataat taaattttaa acagaaatac tgaaatttta aattcgaaag
     9061 cattgaattt tggataaata cattttaaat agaaaaatac gctctcaaaa tgaagtcatc
     9121 tctaaaagaa acgatttaaa gatgactact gagagcgtag cataatggaa gaagtgtgca
     9181 gggtgtctaa aaatgcaaca atacaaaggt agttgcaaga caagttgcct tatctagacc
     9241 atttgtgttc tatgcgacca aacttccaaa ttaaacttga aataagccaa gtaattaaaa
     9301 ataatgcaac taaaatatag cctaaataat caaattcgat cgaaccaatg aatgcccaaa
     9361 acgcaccatg taaatctaac ttatcagcaa gaatttgtag caattcaatc atcccaatca
     9421 ctaatgctgc catgactgat atcgcagtaa tcgttatatt gtaatagatt ttgcgaatag
     9481 gattgaagaa tgcccaatta taggcatact tcattacaac accatctaat gtatccaata
     9541 aactcatacc tgatgcgaat aaaattggta aagataagat tccgataaat gaaatggctt
     9601 gttgtgatgc gcctgaagaa agagcgagta acgcaatttc actagctgta tcaaaaccaa
     9661 gtccaaataa aaagccaagt ggcaatacgt gccaactacg cgtgattaat ttgaaataag
     9721 gtcctacaaa tcgagaaacc aatcctctag attcaagtaa tgcatcgact tcagcttctt
     9781 caatgtgttc acgacgtaat ttagcgaaca agttaattaa agagattaaa ataattagat
     9841 tcaacacacc gataagcact aaaaagaaac ctgaaactag tgtaccaatc gttccaccaa
     9901 tatcttggaa atgcggtaat tcatctttag cccattttac agatacccct aaaaaaacag
     9961 ccattaaaaa tacgacagat gaatgtccaa ttgaaaaata gaaacccaca ccagatggat
    10021 ctttgcgttg ctgtaataat ttgcgaaccg tattatctat tgcagcaatg tgatctgcat
    10081 caaatgcatg acgcaaacct aatgtatatg caagaatccc cataccaaat aagatatgat
    10141 ggtcttttcc agcaatccat aaaaaactaa acccaataac gtgtaacaaa atgacaatag
    10201 ctatgtatgg taaccagctt aagcgctcat ttttaaaaac agtcaaaata tatccttctt
    10261 tcataacatg atatttaatc atactgtatg ttcaatgggc actctagtaa taagtgttca
    10321 tataacaaaa atgttatgcc aaattatttg ttatataaaa atatacatgt aaccacaaaa
    10381 gattttttgc gatatatata atttgataaa ttaaccaaca acaatgtaag atgtcacttt
    10441 gcttaacttg gcatcctttt tatgattttc aaattcaaaa aaatgagcaa aatgaatctc
    10501 tttaccagtt tttaatattt caataccatg catggaacct aagcacccat gtgtgatgct
    10561 ggaatggata ttgagactag caacctgatt gtaatgatta gatagttctt gaattaatat
    10621 ttgaggtccg tatatgtcaa agcggccagg gacagaccaa ataaattctg ttgtaaccag
    10681 tgaacgtaat aattcaatat ctaatgctgc tgtaacaact ataaaatcta tcatttgttg
    10741 acgtttaggc gcatgattgc atgacacatc tcctgttaac ttaaaaggta atgatgactg
    10801 aacttccgtt ttaaaatgta gttggtgctg aaataaagct tgttccaata ttgatatagc
    10861 tttaggacca acgccatgca tctccattag agatgacttc gtatattgtg atactgcttc
    10921 taatgtgtat ataccttgtg aatttagcgc gcgtgttgca ggctttccaa ttttaggtaa
    10981 agtcatttgt atccatcctt tatgttgtat ggtagtcttt ctggcaatat accctgattt
    11041 gtacagaatt atttataaaa ttaatattgt ttagtggttg gtgaataaat gtgggaaata
    11101 taaacaaaat gatatcttta tcgaaatatt attagtaaaa tcgttatatt agcataacca
    11161 agtgagcgta tgcagtaaat taaaaattag gaaagtaagg aggtttgaag gtgaattcag
    11221 agtataaaaa aggcatattt ttagcactca gtgcatacat tctgtgggga atactaccta
    11281 tatattggca gttcgttgat gcaataggcg catttgaaat tttagccttt cgtattatat
    11341 tttcagcaat attcatgatt ttcatactcg cggttggaca aaaacaacgc aatgcatttc
    11401 aacgagatat gaatcaattg ttaggcaagc ccattcagct attagcgatt gtcgtagcag
    11461 gctatgtcat tacattaaat tggggtacat ttatttgggc tgtaacgaac ggtcacgtcc
    11521 tacaaacaag tttaggttat tatataaatc cacttgttag cattttgctc gcacttatct
    11581 ttttaaaaga aagattcaat aaatttgaat ggctagccat tttattcgca ttcatcggtg
    11641 tattatatat gacgctcaag attggagaat tcccaatcgt ctctattata ttagcgttat
    11701 cctttggtac atacggatta ttgaaaaaag tagtacatat tgatgccatc agcagtatta
    11761 cgattgaatg tattgttacc gcacctgctg gactaatata cgttatttat ttatggcagc
    11821 aacatcagat gtcatttgga ttgaacatgt catcattttg gttgttattt tctggtgcta
    11881 ttacggcaat accactaatc ctattctcag ccggggcaaa acgtattcca ctttcgctaa
    11941 taggatttat tcaatacgtt ggaccaacaa taatgtttgt actcggcata tttgttttca
    12001 aagagccttt tagtatagat caattaatta cgtttatatt tatttggaca ggtattgtgt
    12061 tatatagtct ttctcaatac attaaattga agaaacatcc ggtcgcaaaa accctataaa
    12121 atacgaggaa agataattat acgtgttttc aatatccttt gtgtcatgat gaggtgagac
    12181 tgaggtatgt gtgcagtaac agtgacagac gtataaattt gaaacattaa atatgaaata
    12241 aggcactttc gttgaattcg caattaaaat gaatttaaac gaaggtgttt tttgtatgat
    12301 gatttatgag ggcatacgta atgttaatca atgaagcgga atagtataga aatgtatttt
    12361 atgtgatgtt acgtaatgac ttaaatcttt atacaaattt tcagtgaagc acgcaatgat
    12421 ttaaaggata aacgtatttt aaaaagggag tcttgattta gcaaaggttg attttaaata
    12481 caaattaaaa agcgcccaaa tcaatgagat tcgagcgcta atttaaatat tttaaaatat
    12541 accaattact ttcatccaaa gtgatcctaa accaatccag ataataaagt agacgaaacc
    12601 taatattaag ttcattgtcc accaacgttt ttgagtcacg taacctgaag agaatagaat
    12661 cggcgctgga ccactactat agtgtgttgt tgaagctaat aggttaccga agaaacctaa
    12721 cattaatgca ctgaataatg gtggtgcacc ggctgcgatg gcaacgccta gtaatgctgc
    12781 atacatcgca ctgatatgtg ctgtagaact tgcaaataag taatgtgaat agaagtagaa
    12841 caatattaaa atgaccagga ctataggcca gcttaagcca ccaagacttg tagcaatgga
    12901 tttacttaac caaggaataa atccaagctt gtttaattgg tcggccatta acactaatac
    12961 tgagaaccat actaatgtgt tccaagcacc tgtttcgttt aaaatgtctt gccatgttaa
    13021 gacgcctgtc aataataaca atgctagcgc aataaaggcc gttaaagttg catcaatatg
    13081 aatgaaactt ccgacaatcc atagtgttaa cgcaacgaca aaaataccaa tcataaattt
    13141 ttcagctaaa gcgattttac ccatagtcgc taattcattt tcagcccaac tcttagcatt
    13201 tggtgtttct ttaacagttg gtggataaat tttataaata ataaaaggta caacaattaa
    13261 ggaaacaagt ccaggaacta aagcagctag aaaccaattc atccatgtaa tgtgaacatt
    13321 agatgtgcta gatgctaaat tttgtgcaag ggggttaccg gccattgcag ttaaaaacat
    13381 agccgcagta attaaattac cttggaattc tgtgaaaaca agaaatgcac ccattttgcg
    13441 tgctgatccg tctttcggtt tcgaaccaaa tgattcagaa agtgatttga taattgggaa
    13501 catgattcca ccagcacgcg cggtattact tggtgtagca ggcgctagaa ttaaatctac
    13561 accgacgata gaatatgcta atcctaatgt ttttttacca aataatttga cgaaatgaag
    13621 tgcgatacgt ctaccaagac ctgttttcac aaatcctctc gaaatgaaaa atgccatagc
    13681 aattaaccaa atgctattat taccaaaacc agcgacagcc gttttcatgt caacaatgcc
    13741 aacgagtacc atgattgtaa atccaattat agagacggcc ccaattggca tcggttgtgt
    13801 aatacaagca atgattgtcg cgacgaatat tgcgaacata taccatgctg ttggatccac
    13861 agcatccggt ttaaaaggtg taagtgccca aataaggaga cctacaacga tagggagtat
    13921 aaacttacga tatttaaccg tgttttccat gttaaaacgt ccttctttct atgttttata
    13981 catatttcaa tttaagaata aagctaacta caaaagatgt acagtaataa ttaaatataa
    14041 aattaaatta acgaaatcat taatataatt atttttcgag aatcggtgaa gaactggtat
    14101 agatggtgtt tattaaattc aaaaaagtga ccttttaaac aaataaaaaa cccgttaacg
    14161 tgttgttaac ggttaataag cacatattta ttttaaattt tttaatatac gagacatctc
    14221 ttcaatttta ccagcatgaa gtgggatgtg aatggcattt aatactaata actcttctaa
    14281 gttattacaa ccgacaattg gctgtttcaa ttcaattgct aaatcatctt cagttagatt
    14341 tcgtgcacgt tcaggtaaag tttggagacc ttctaaaatc ctttcaatac tcggtacttc
    14401 atccttccat tcatctggag acgaaccatt tccgaataaa ggtctataga tatttaaatc
    14461 aatattctct ttaccagcaa cagctaaggc cgattcaaaa attgttaata catgaccaaa
    14521 ttgccaatga atggtattag aaaaaggctt atgataatca tcaagtacct tttcaacttt
    14581 ccaagcatca tacacattta ctaaatatcc taaccccata tcaatgacat caaatactgt
    14641 ttttgtagtc attattaatt cctcccccat aaataaaata tacttccgta agtattaccc
    14701 tattaacaaa gttgttagtc atttaaggaa tgtgtacaac aactacctta caataaaaga
    14761 tagttgacga aaagttctca atgtactatc ttattatgta agatagtagg tgattaaaat
    14821 gtcagtgtca gatcagatta tgaaaggtct cttagatggg gcgatattag gtctcattgc
    14881 ccaaggcgaa acgtatggat atgaaattat gacgaaactt aagaatcaag agtttccaga
    14941 gataagtgag ggtagtattt atccagtttt aatgcgttta aacaaaaaag gatacgttga
    15001 tacgacaata aaaaaatcta gtaatggcgg cccgaggcgg aaatactata cgattaccga
    15061 ttcaggttta aaggaactaa aagtatttaa atcgaaatgg caagttttga ataaagggat
    15121 gattaacttg tttggagggg aaaacgatgt taagtaaaca atctcaagat tttttagaaa
    15181 aattacgtgt tgaattgtta tttagaggta agactgaagc agaagtagaa gagttattgg
    15241 aagagctcga agatcattta actatggctg aaaaaaataa cgaagataca agttcaatta
    15301 ttaatacacc gattaagtca atggctgatc aattatcacc tgagataagt cttactaccg
    15361 gtttgtataa atatattact ttgtacttcg cttttatttt agcagtcatt ataattccta
    15421 ggtttttaga tttgggtaca tttgatgtga caattgcatt tttactttat atagctagta
    15481 tcgttatttt aggtgttgtc attggtatgc tgattctacg aaatacatta gtccgatttg
    15541 gtgaccaaaa aataacgtat gttataaatg tgacttatgg aattatcgta tttttatgga
    15601 tgattttagg tggcttgatt attaaaaaat atcctatcta tagtttcttt gagttaagtg
    15661 acaaacagag tttcatcgtt gggttagtac ttttaattgc gctcattgga acttgtattt
    15721 taattaaaca gaaaatttat gcgctcatcg tgttggtaat ttcattgcca agtttgatag
    15781 caaggatagc agctatcttt agtaaagaac cagacacttt taatttagtt tcagtaagta
    15841 tactcatcat tctaaatata gcatttatta tttatacatt tgtaaatttt aggaagttaa
    15901 aaaaagaaga ctaaacaatg tcgtttagct aaaatacatc attatagtaa aactgtatac
    15961 caatcatcca aaagcaccct aaagttagac ttaaactaac aaattagggt gcttttgttt
    16021 gtgcacttat aactattccg ttgtgcccag ggctaggtgt aaatcctcat cattagaaat
    16081 agcttcaatg gctgcagtca aacctgcaac tatcttttct aatggcatag atggtgtatc
    16141 gggtttacca atgacctgct ctggtatgta tggcacatga ataaatccga atcgtaggtg
    16201 agggtaatgc ttatcttgta aataacctaa gtgataaagt gtgtgattac aaacaaatgt
    16261 acctgcgcta tttgaaagtg ctccaggaag tccttgatta ataatacttt gagtcattgc
    16321 tttaactggt aaatttgaaa aataagctgg cgcaccgtct aagtgaatgg cttgatcaat
    16381 aggttgaaaa tcatcattat ctggaatacg tgcatcatca atattaatgg cgacacgttc
    16441 tggggtaatg gcatttctac caccagcttg tcctatagct agtacaacat cataatgatt
    16501 agatgccaac gttttattta taatattatc tactttctta aaagaggttg gtagttttaa
    16561 tttatcgatt gtatgtgtgc caataatatc ttctagttga gtcacagctt cccatgaggg
    16621 attgatattt tgattgtcaa aaggcgcaaa ccctgttact aaaatgtgca ttttaagaca
    16681 ccccgcagta ttaaaattta atttagtata acacgcgcat aattaaaaac atatcaggtt
    16741 atgtatgcgc ataaaaaaca gccttgaaaa acctttataa atcggatttt caaagcgtgt
    16801 tttgaaaggt atttaattaa aactaagcat gttgatgtaa atcatcaaag tttgttaaac
    16861 gttgttgcca ctcattatca ctaatattat ttgcttgaac ataacgatta cgctcatgtt
    16921 tagcacattc ataagagcat gcacctaaat atttagtttc gttttcttca gaaactaata
    16981 tttgtttatt acattctggg ttagcgcaat taatataacg ttcacatggt ttgccatcaa
    17041 accaatcctt accaataatt gttttttcaa cttggttgat atcaacactg atacggtcat
    17101 caaatacgta cattttaccg tcccaatatt caccttttgt ttcaggatct ttaccatatg
    17161 tagcaatacc gccatgaagt tgagctacat cttcgaaacc ttcttttaaa agccatccag
    17221 aaaatttttc gcatcgaatg ccaccagtac agtacgtaac cactttttta tctgcaaata
    17281 acgctttatt ctctttaatc cagtcaggca aatctctaaa acgtgtgata tttggacgaa
    17341 ttgcacctcg gaaatgacct aaatcaaatt cataatcatt acgtgcatca ataatgactg
    17401 tgtcatcatc ttcaagagct tttctaaatt ctacaggtga taaatattgg ccagttgtgt
    17461 gtcttggatc gacgtcatct tctaaatcta aagcaacgat ttcttttcga ggacgtacat
    17521 gcattttctt aaaagcatgt ccttcagctt catcaatttt aaacaccata tctttgaatc
    17581 gttcatcggc atgcatatgt gccatatatt gttcggtttc ttcttttgta ccagataatg
    17641 tgccgttaat accttctgta gaaacaagaa ttctaccttt taaatggtgt gctttacaaa
    17701 aggctaagtg atcctgagca aactgttcag ggtcatcaat cgtcatatat ttataatata
    17761 aaagaacttg atagttcata ggttaatctc cttctataaa catgttataa gcatgttaat
    17821 ttttaaaata aagcgtcaat acgcatattt gtctacgtcc atcattttaa cagagttgta
    17881 ttgtcattca atatttctag cttgaaatca gccactagac cgataaaata gatagatgaa
    17941 accgttatca ttttatttga tagttatctt gtttaaaata aaattgatag ttaaaacgaa
    18001 tgaaatgaaa ggaatgatca tgatgtcaca acaagattta cctacattat tttatagcgg
    18061 gaagtccaat agtgctgttc caattatatc tgaaagtgaa ttacaaacaa ttacagctga
    18121 accatggctt gaaatttcca aaaaaggatt gcaactagaa ggattgaact ttgatcggca
    18181 gggacaactc tttttattgg atgtattcga aggcaatatt ttcaaaatca atcctgaaac
    18241 gaaggaaatc aaacgacctt ttgtaagtca caaagcgaat cctgcagcaa tcaaaataca
    18301 taaagatggc cgattattcg tttgttattt aggagatttt aaatctacag gaggcatttt
    18361 tgcagctaca gaaaatggtg acaacttaca agatattatt gaagatcttt caacagcata
    18421 ttgtattgat gacatggtat ttgattctaa aggtggattt tattttacag attttagagg
    18481 atactctacc aatccactag gaggcgttta ttatgtttcg ccggacttta gaacagtgac
    18541 gcctatcatt caaaatatta gcgtagcaaa tggtattgct ttaagtacag atgaaaaagt
    18601 actatgggta acagaaacaa cagccaatcg attacatcgc attgcacttg aagatgatgg
    18661 tgtgacgata caaccatttg gagctaccat accgtactat tttacaggtc atgaaggacc
    18721 agactcatgt tgtattgata gtgacgataa tttatacgta gcaatgtatg gtcaaggtcg
    18781 agtgttagtt tttaataaaa ggggttatcc aataggacaa atattgatac caggccgaga
    18841 tgaagggcat atgttacgtt ctactcatcc gcaatttata cctggaacaa atcaactcat
    18901 catttgttcc aatgatatag aaatgggcgg aggatctatg ctttatacag ttaatggctt
    18961 tgcaaaaggt catcaaagtt ttcagtttca ataagaccta cctgaatgaa aggcgtctaa
    19021 aattagggtg aacagttagt gcttgttaca catgagttaa acgaaaaatg ccaactatac
    19081 ctagggatga tatagttggc atttcgtata tatattaaaa caatgaggat tgtgtaatga
    19141 gattattctg agattgagaa ttcagctgat gcttcgaatt taacatcttt gcctagcatt
    19201 acgccaccag tttcaagtgc ttggttaaag ttaatgccat atttttctct attgattgta
    19261 ccagtaacaa taatacctgt tacttgagaa ccatccatag gattcttact tactccgttg
    19321 aattcaacat cgaatgtttc ttcgttagtg atgcctttaa ttgttaaatc accaacaact
    19381 ttgctttcag atactgattt tgtcacaaat gtaattttat caaattcgtc agtaccaaag
    19441 aaatcaccag attttaagtg gttatcacgt gcttcgtttt tagtgttaat tgagcttgga
    19501 ataattgttg cagtagcttt caaagtactg aagtcattaa tatctccttc aacagctaca
    19561 tcaaattgat caaatgaacc tttcacttta gaaaccatta aatgtttaat ttggaattct
    19621 aaactactgt gtgcaccatc aaaagtaaag ttagtcataa tataaaacct ccatattttt
    19681 atctcttaga tatatttttt atacttgata tacatacaat accacctaaa aaacaaagat
    19741 gcaagcattt tatttcgaat tcgaatattt tctgtgatgt atttaaagaa tgacctataa
    19801 aacaccacac tattgttata ccctagtcaa ttaattcaaa cataaagaag tgattacaag
    19861 tccgtgaaat ttagttaatg agataaatct gactattatt atcattttta tagtcactac
    19921 tattgacttt gcatcaagtg actcgtacta ttttaatata attctgaaaa ttcaaaatat
    19981 agaacattaa atatgtagat aatatttttt tgaagtttta aaaaaaggag gcattttatg
    20041 ggaaattatt tggtattgca atcagatttt ggacttagtg atggcgctgt aagtgcaatg
    20101 tatggagttg catatactgt aagtgatgat atacgtgttg aaaacttaac gcatgatata
    20161 ccaccgtatg acatttgggt agcgtcatat cgtttatatc aaactgtaaa atactggcct
    20221 aaaggcacag tttttgtatc agtggtagac ccgggtgtag gtagtgatag gcggagcatt
    20281 gcttgcttaa catattcagg tcattacatt attacacctg ataatggttc tttaagtcat
    20341 attaaacatt acgaaggtat taaaaaagtt atcgaaattg atgaagtgaa aagccgattg
    20401 cctcattcgg aagaaagtca tacttttcat ggtagagatg tttatgcata caatggtgcg
    20461 agattagctg caaataagat agcgtttgaa cgtctcggtc aggcaattaa tgttgatagt
    20521 attgaggcat tagaaataag agaagtgaca aaaaatgagg attctgttac aggaagcatt
    20581 gatattttag atatcagatt tgggtcatta tggacaaata ttccattggc attcttaaaa
    20641 tccttggaaa ttgttcatgg taataatttg gtggtaacaa tttatcatca agataagaaa
    20701 gtctatcaaa atattattaa atttgcgcga tcgtttgcag atgttaatgt gggtgaacct
    20761 cttgtgtatg ttaactcgct agtaaatatt ggtgtagcag tcaatcaaga ctcattttca
    20821 gatttatatc atattggtac tggaaatgat tggacgattc atttatctaa agcaccatca
    20881 atataatgaa ttttttatgt aaggagtaat gaagatgaaa aaacaagata tttcagttaa
    20941 aactgttgtt gcaattggta taggagcagc ggtatttgta attttagggc gttttgttgt
    21001 aataccaaca ggttttccta atacgaatat agaaacatca tatgcatttt tagcattaat
    21061 atctgcaata tttggacctt ttgctggttt aatgactgga ttagttggac atgctattaa
    21121 agattttacg acatacggta gtgcctggtg gagttgggtt atttgctcgg gaattatagg
    21181 ctgtttatat ggctggattg gactaaaatt aaatctttct tcaggccgat tttctaggaa
    21241 atcgatggtt tattttaata tcggtcaaat tattgcgaat attatttgct gggcacttat
    21301 tgcaccaaca ttagatattt tgatttataa cgaaccagct aacaaggttt atacacaagg
    21361 tgttatctct gcagtattaa atattatttc agttggcatt attgggacaa tattattaaa
    21421 agcatatgct tcatctcaaa ttaaaaaagg tagtttacgt aaggaatagt tattttgttg
    21481 aatcagatat gtaaatgaat atagaaaggt aatgatatat catgactgaa ccaattatct
    21541 cgttcaaaga ctttagtttt caatatcata gtcaagcaac acctacatta cagaatataa
    21601 atgttgatat ttatccagga gaaaaagtat tagtagttgg tgcttcgggt agtggtaaat
    21661 cgacttttgc aaattgcata aacggattaa ttccatttaa aactaaaggt aacataactg
    21721 gggaactata tataaataat caagatgcaa ccgttagttg tttacatgat agatctaatg
    21781 ttgttggtac agttttaaaa gatacagatg gacagttcat aggcttaaca gcagctgaag
    21841 atatggcctt tttattagaa aataattgtg ttgaacaaga tgatatgaag aaaaatgtaa
    21901 gttattgggc tgaaaaagtt ggcatgatag aacatttaaa tcaccgaccg caagatttat
    21961 ctggaggtca aaaacaacgc gtttcattag gtggtatatt aatccatcgt acgcctattt
    22021 taatattgga tgagccactg gccaatttag atcctgcgac aggacatgaa acgctgagat
    22081 tgttaaacaa tattcatgaa gaaacaaagt caacgatgat tattgtcgaa catcgattag
    22141 aagaatcatt agatgatacg tttgatagag ttcttctttt taaagatgga aaaatcatcg
    22201 ctaatacaac gcctagtgat ttgctaaaat catctaagct taaagaagca ggcatacgtg
    22261 aaccattata ctgtacagcg cttaaatatg cagaagtaga tgtcgaatcc attgataact
    22321 tagcgaattt acgtgacgtt tgtatgagtg aacatgttaa atttaaagtg aaaaaatgga
    22381 tagatgaaac atctgcaaat aatgataata agtacaaatc tgaaccgcta ttagaattaa
    22441 atgaggtatg tgttcaatat agtgactata gtaatagtgt acttaataat gttcaattaa
    22501 atgtttatag aagagaaatg cttagtatag ttggtcacaa tggtgccggc aaatcaacac
    22561 ttgctaaagc aatttgtgga tttttagata taacaggtaa tatccaattt tgtaataggg
    22621 gatttaatca attgtcaatt agcgaacgat ctgaattcgt aggttatgtg atgcaaaatc
    22681 caaatcatat gatttctgaa aaaatgattt acgatgaagt agcattaggg ttaagagcac
    22741 gaggtatgaa agaatcagat ataaagatac gagtagagaa tgtgctcaaa atatgcggac
    22801 tttatgcatt tcggaattgg ccaattgcag ctttaagcta tggtcaaaaa aagagggtca
    22861 ctatagcatc tgttttagtc ttaaatccgg aaataatcat attggatgaa ccgactgctg
    22921 gtcaagattt ctatcattat aatgagataa tgtcattttt aattgaacta aacagacagg
    22981 ggaagacgat tattatgatt acgcatgata tgcatttatt gtctgagtat agttcaagaa
    23041 cagttgtatt atcaaaaggt caagtcgttg ctgataccac gccagtattg gttttaaatg
    23101 ataaaaaaat ctgtgagatt gcatcattga gacaaacatc gctatttgaa atggccgaat
    23161 atatagggat tagcgagcca cagaaattag tacaattatt tattaaccat gataggaagg
    23221 tgagacgcca atgaatcaat ataatactat aggttttcac ccgggaaata gtcgtattca
    23281 tcaattaaat gcgactgtta aacttttatt cttattagtt gtttctattt ctgcaatggt
    23341 gacttatgac acaagatatt taattttaat tagtgcttca tctattttat tggtcaaata
    23401 tgctcatatt gaatggaaac aagttcgctt tgttgttaaa ttcattctgt ttttcacaat
    23461 attaaatatt attgccgtgt acatatttga ccctgaatat ggtgtgaaga tttataatca
    23521 gcgtacagag ttagtcaatg gtattggtcg atttacgcta acatcacagg aattattcta
    23581 tctttttaat ctaatattaa aatatattag tacagttcct ttagcgttaa tatttttatt
    23641 cacaacgaat ccgagtcatt ttgctgcaag tttaaatcag ctaggtgtga attataaaat
    23701 cagttacgca gtctcactag cattaaggta tattccagat attcaagaaa catattttaa
    23761 tatttcacaa gcgcaacaag caagaggata tgataattcg aaaaaagcaa aatttactag
    23821 ccgtgttaaa ggtatcaaac gcattgtgtt acctttaata ttttctagta tcgaaagaat
    23881 tgacactatt agtactgcta tggagttaag acaattcgga cagtataaaa ggagaacctg
    23941 gtacgtcaaa aaacaattaa aaaaagatga ttatgttgtt ttgtgtttga cgttaatact
    24001 tctgatgtta gtagttacat tattcttttt aaataatagt cgatatttca acccgtggca
    24061 ttagtattca tataaatagt ctttaaatag aaataggagg gagacattta atgataaata
    24121 ctgaaagatt aaatttaatg attccaagtt cctcgcattt aattgaactt tataatattt
    24181 gtagtcatcc acaagcaaat atatacactc ccaaaggttt acataattcc aaattagaca
    24241 cacaacggtg gattgaaaaa tggcgaaacc attggcaaca atatcaattt ggttactttg
    24301 tattggtaaa aaaaatagat tgtagtgtta ttggtatttg tggatatgaa tatcgacaat
    24361 taaagcaaga aacagtatta aatttatttt ataaattaca tccaagtttt gaaggacaag
    24421 ggtacgcatg tgaggctatt acagcaatca caaattttgt gaattatatc gatcaagaaa
    24481 cagtaaaagt tatcaggaca aataagtgta accaacgttc aataaattta gcagaaaggc
    24541 ttaaattcaa gcgagacgat actatggacg acattatcaa tcaaggagat attgtgtttt
    24601 ataaataaaa tactatgaca ttatctaaaa aataaaatta aaatcccaac agtttcttaa
    24661 aaagttatga gccacataat aaaaatgtta taattgtgca taataaaaca tagggagaat
    24721 gtttaatgca taaaaaatat ttattgctat tttttctgtt attatcagtt gcattgttat
    24781 atagctgttc caagcaaaat gagaaagctc aaaataaatc tgttaatcca aatgagtatg
    24841 caaagcataa atcgaaaatg gatgaacatt gggaaacata tcacggccaa gtttatcatg
    24901 tgttttatca cccattaatt acagacccaa aagttgcgtt tacaggagat acacaccaag
    24961 ctaagggcaa caatgattgg atgattactg taagtgaatt taaaaaatca ttgaacgaat
    25021 tatataaaaa taattacatc atcgttaatc cacatgatgt atacgactta tcatctcatc
    25081 atgtaaaagc caaaaaaata aaattgccga aaggtaaaaa gccactaatc atctcaattg
    25141 atgatatgaa ttactatagt tacatgagac accatggtta tgctgaccgt ttagtactcg
    25201 ataaaaataa acatgttgtt tctgaaacta cagacaaaaa aggccatgct acttattctg
    25261 atgacaatga tatcgtgcca attttaaata aatttgtaaa agagcatcca gatttttctt
    25321 taaatggtga aaaaggtgtt gttgctttaa ccggttatga gggcgtatta ggttatcgca
    25381 cgaatgaatt aacgagtaaa gattatacaa agaataaaaa ggcagctgag gaagttgttc
    25441 gcgcaatgaa acgggatgga tggtcatttg cgagtcattc atatggtcac attaattttg
    25501 aaaaaacatc tttagaaggt attaaacgtg atactaaaag atggaaagat gaagttgaac
    25561 caattgtagg taaaacagat atgtttgtat ttccacatgg cgcacaggat agacatacac
    25621 aagcgtatga ttatttggta gatgaagcag agtttaagtt tattgcaggt gtcggtccaa
    25681 acaattttac agatatatca gcaacgaacg tttaccaaga tagggttgcg attgatggtc
    25741 taaacttatt tgaatttaaa tataaactca aaccgttttt taatcctgaa aatgtatata
    25801 gtaaacaaga tagacgttac tttaaaggga atcgggatta tgaagaatag ttaaatttaa
    25861 taaaaagata gaaagaaaat aactttacat aataaggcaa ggacaatatg tgcaagaagt
    25921 acaacttcaa tataaaaatc ttggttaagc atttttatag gcacaaatgt cttgcctttt
    25981 ttattttaaa aaattttaaa atcaatacac cgaattttct gaaaaaggtt gtaatgtatt
    26041 gctaaaaatg ataagttatt aatcatatta cactttagcg tggtagagaa gtaaagcaaa
    26101 caacgaaaat accaagtgat tttaaataca gtattgaaag taaagggtgg tcgagtgaat
    26161 aattcagaac aattaattgc gttaaaagaa tcggaaacag catttttaaa atattttaat
    26221 aaagcggatt atgagttagt tgattttagt gtggttgaaa agttggactg gaaacaattg
    26281 aaccatgagg atttgcagca gatgggtgag cgaaattttt ggcaacatga gcatcaaatt
    26341 tatgcacttc gaaatgattt tacagatcaa ttattgcgtt attactcaat gtatccaact
    26401 gcagcaacaa aagtggcata cacaggttta atcattcgga ataatgaagc ggctgtacaa
    26461 gttggtttgg aaaactatgc accatcatta gcgaatgtgc aacagagctt aaagctcttt
    26521 attcagttta ttcaacagca gttgagagac aacgtgcatt ttgttgtatt gggacattat
    26581 caattgcttg atgccttact cgataaatcg ttacaaacac cagacatact atcaatgatt
    26641 gaggaacgta atttatcggg actggttaca tatttgtcga ctgaacatcc aatcgtgcaa
    26701 atcttgaaag agaatacgca acaacaatta aatgtattgg aacactatat tccaaatgac
    26761 catccagcac tcgttgaatt aaaaatatgg gaacgttggt tacataaaca aggttacaaa
    26821 gacatccatt tagatattac tgcgcagcca cctagatcct attacacagg tttatttatt
    26881 caatgtcatt ttgctgaaaa tgaatctcga gttttaacag gtggttatta caaaggaagc
    26941 atcgaagggt ttggattagg attaacactt taagtaaggg agtatgcaca atgttaagaa
    27001 tcgccatagc caaaggacgt ctaatggata gtttaattaa ctatttagat gtaattgaat
    27061 atacgacatt atcagaaaca ttaaaaaata gagaacgcca attattatta agtgtagata
    27121 atattgaatg cattttagta aaaggaagtg acgtgccaat ctatgtggaa caaggaatgg
    27181 cagacatagg cattgttggt agcgacatat tagatgagcg ccaatataat gttaataatt
    27241 tgttgaatat gccttttgga gcatgtcatt ttgcggttgc agcgaaacct gaaacgacca
    27301 attatcgtaa aatcgcaacg agttatgttc atactgctga aacatatttt aaatcaaaag
    27361 gtattgatgt cgaattgatt aaattgaatg gctctgttga attggccggt gttgtagata
    27421 tggtagacgg aattgtcgac atcgttcaaa caggtactac gctaaaagcg aacggactgg
    27481 ttgaaaagca acatattagt gatatcaatg caagattaat aactaataaa gcagcttatt
    27541 ttaaaaaatc acaattaata gagcaattta ttcgctcttt ggaggtgtct attgccaatg
    27601 cttaatgcac aacaattttt aaatcagttt tcattagaag caccattaga tgagtcattg
    27661 tatccaatta ttcgcgatat ttgtcaggaa gttaaagttc atggagataa ggctttgaaa
    27721 atgtataatc taacattcga tcatacgaaa acagatcatt tagaaattag tcatgaacaa
    27781 attaaagcag catttgacac attagatgaa aaaacaaaac aagcattaca acaaagttat
    27841 gaaagaatta aagcatatca agaaagtatt aaacagacga atcaacagtt agaagaatca
    27901 gtggagtgtt atgaaatata ccatccacta gaaagtgtcg gtatttatgt gcctggtggt
    27961 aaagcaagtt atccttcaac ggtattaatg acagcgacgt tagcacaagt agcaggtgtg
    28021 gaaaatattg tcgttgtgac accacctcaa cctaatggtg tgtctcaaga agtattagca
    28081 gcatgctata ttacgcaagt tgatcaagta tttcaagttg gtggtgctca aagtattgct
    28141 gcgttaactt atggcacaga aacgatacct aaagttgata agattgtagg tccaggcaat
    28201 caatttgttg catatgccaa gaaatattta tttggacaag taggtattga ccaaatagct
    28261 ggaccaacag aaatagcact tattattgat gaaacagcag atttagatgc catcgtatat
    28321 gatgtttttg cacaagcaga acatgatgaa ttagcacgta catatgccat tagtgaagat
    28381 gcgcaagtcc ttaaagattt agaatcacgt attgctaaag cattgcctaa tgtggacaga
    28441 tacgacattg tttctaaaag tatcgctaat caacactacc ttatccatgc tagtaatttt
    28501 gatgaagcat gccatgtcat gaatacaatc gcgcctgaac atgcgtcgat tcaaacagta
    28561 aatcctcaac catatattga aaaagtgaaa tatgtgggtg cattgtttat tggacattat
    28621 tcgccagagg tcataggaga ttacgttgca ggtccaagtc atgtattacc tacaaataga
    28681 acagctagat ttaccaatgg gttatcggtc aatgatttct taacacggaa cacggtcatc
    28741 catttatcaa aagatacgtt tgaacaaatt gctgattcag cacaacatat tgctcatgtt
    28801 gaagcattat acaatcacca gcagtctatt ttaatacgtc agtcttaggg gagtgtaatt
    28861 gaaatgattt atattgataa aaatgaaagt ccagttacgc cgttggatga aaaaacaatg
    28921 acgtctatta ttagtgcaac gccatataat ttatatcctg atgcagcata tgaacaattc
    28981 aaggaagctt atgctaagtt ttacggatta tcgcctgaac aaattattgc aggaaatgga
    29041 tctgatgaat tgattcaaaa gttaatgctg atcatgccag aaggtccggc attaacgcta
    29101 aatcctgatt tttttatgta tcaagcatat gcggcacaag taaatcgtga aattgcattt
    29161 gtagatgcag gatcagattt aacgtttgat ttggaaacca ttttaacgaa aatcgatgaa
    29221 gtacaaccat cattttttat tatgagtaat ccacataacc cttcaggcaa gcaatttgat
    29281 acggcatttt taacagctat tgcagataag atgaaagcat taaacggata ctttgtcatt
    29341 gatgaagcat atttagatta tggtacggca tatgacgtgg aactggcacc acacatctta
    29401 agaatgcgta cattatcaaa ggcgtttgga attgccggct taagattagg tgtcttaatt
    29461 agtactgctg gaacgataaa gcatattcaa aaaatagaac atccatatcc attaaatgta
    29521 tttacgctaa atattgcgac ttatattttt agacatagag aagagacaag acaattttta
    29581 acgatgcaac gacagttagc tgagcagtta aaacaaatat ttgatacaca tgttgcagat
    29641 aaaatgtcag tgttcccatc aaatgctaat tttgtactta ctaaaggctc agcagcgcaa
    29701 caattaggac aatacgtata tgaacaagga tttaaacctc gcttttatga tgagccggtg
    29761 atgaagggct atgtaagata ctcaattgca acagcatcac agttaaagca attagaagaa
    29821 attgttaaag aatggagtgc aaaatatgat ttatcaaaaa caacgaaaca cagctgaaac
    29881 gcaactaaat atttcaatat ctgatgatca gtcaccatcg catattaata caggtgtggg
    29941 ctttttaaat catatgttaa ccttgtttac atttcatagc ggtctgtcat taaacattga
    30001 ggcacaaggt gatattgacg tagatgatca ccacgtaact gaagatatcg gcattgtcat
    30061 tggccaattg ttacttgaaa tgattaaaga taaaaagcat ttcgttcgtt atggaacgat
    30121 gtacattcca atggatgaaa cattagcacg tgtcgttgtg gatataagtg ggcgcccata
    30181 cctatcattc aatgcatcat taagtaaaga aaaagttggt acgtttgata cggagttagt
    30241 agaagaattt tttagagcgg tcgtaatcaa tgcaagatta acaacgcata ttgatttaat
    30301 tcgtggaggt aatacacacc atgaaattga agctatattc aaagcgtttt cccgtgcatt
    30361 aggcatagcg ctaactgcaa ctgatgatca gcgtgtgccg tcatcgaaag gtgtgattga
    30421 atgattgtca tcgttgatta tggattaggg aatattagta atgtaaaacg cgctattgaa
    30481 catttagggt atgaggtggt tgtctcaaat acctcaaaaa taatcgatca agcagaaaca
    30541 atcatattgc ccggtgtcgg ccattttaaa gatgcgatgt cagagataaa acgattaaat
    30601 ctcaatgcaa tattggctaa gaatactgat aagaagatga ttggtatttg tttaggcatg
    30661 caattaatgt atgagcatag tgatgaaggc gatgcatctg gattagggtt tatcccagga
    30721 aatatttcgc gtatccaaac agaataccca gtgccacact taggctggaa taatttagtg
    30781 agtaagcacc ctatgttaaa tcaagatgtt tatttcgtac attcttatca agcgccgatg
    30841 tcagaaaatg taattgcata tgcgcagtat ggggctgata ttccggcaat tgttcaattt
    30901 aacaattata ttggtattca attccatcct gaaaaaagcg gtacatatgg gttacaaatt
    30961 ttgcgtcagg caatacaagg gggatttata aatgattgaa ttatggccag cgattgattt
    31021 gattgggtca acaagtgtga ggttaacaga gggtaaatat gatagtgaag aaaaaatgtc
    31081 acgctcggct gaagaaagta ttgcttacta tagtcaattt gaatgtgtga atcgtattca
    31141 tatcgtcgac ttgataggtg ctaaggcaca gcatgcccga gagtttgatt atattaagtc
    31201 attgaggaga ttaacaacca aagatattga agtaggtggt ggcattcgta cgaagtcaca
    31261 aatcatggac tactttgccg cagggattaa ttattgcata gttggaacga aaggtattca
    31321 agatactgat tggcttaaag agatggcaca tacatttcca ggtcgcattt atttatctgt
    31381 tgatgcctat ggagaagata ttaaagtgaa cggatgggaa gaggacacag agttaaattt
    31441 atttagtttt gtgagacggt tatcggatat acctcttggc ggcattatat atactgatat
    31501 tgctaaagat ggcaaaatgt ccggacctaa ctttgaatta actggtcaat tagtaaaggc
    31561 aacgacgatt cctgtcattg cttccggtgg tattagacat cagcaagata ttcaacgatt
    31621 agcgtcgcta aatgttcacg ctgctattat aggaaaggct gcacatcaag catctttttg
    31681 ggaggggcta aaatgattaa aaaacgtatc attccatgtt tagatgtcaa agatggtcgt
    31741 gtcgttaaag ggattcaatt taaaggatta agggatattg ggaatcctgt tgatttagca
    31801 atgtattaca atgaagcggg tgctgatgaa ttagtatttt tagacatctc taagacggaa
    31861 gagggtcata gcttaatgct agaagtgatt gaacagacag cgtcacgctt gtttatccct
    31921 cttactgtag ggggtgggat tcaaagtctc gatgatatta cccaattgct aaatcatggt
    31981 gcagataaag tatcattaaa ttcaagtgct ttaaaaaatc cacagctcat taaacaagcg
    32041 agtgataaat tcggtagaca atgcatctgc atagcaattg atagctatta tgatcctgaa
    32101 agaaaagcac attattgttg tacgactggt ggtaaaaaaa tgacaaatat taaagtatat
    32161 gactgggtac agcaagtaga acagttaggt gcaggtgagc tcctcgttac aagtatggga
    32221 catgatggta tgaaacaagg ctttgatatt gaacacctag caaatattaa gtctcttgta
    32281 aatattccaa tcattgcttc tggtggtggt ggcaatgcac aacactttgt agaattattt
    32341 gatcagacgg atgtttctgc aggtttagct gcaagtatat tacatgatcg agaaacgacg
    32401 gttcaatcta ttaaagaagt gatacggcaa gggggtatag cagtaagatg accaaatata
    32461 aaattgattt tagcaaaggt ttagtgccag caattttaca agataatcaa acaaaacaag
    32521 tattgatgtt gggttatatg aaccaagaag cttttgataa aacgatagaa gatggtgtgg
    32581 tatgtttcta ttcgcggtcg aaacaacggc tatggacaaa aggtgaaaca tctggtcata
    32641 cgcaacgtgt taaagatatt catgtagatt gcgacaatga cactatttta attgatgtca
    32701 taccaaatgg accaacatgt catacaggca gtcaaagttg tttcaacaca gaagttccat
    32761 tttcagtgca aacattagcg cagacagttc aagatagtgc ccaatccaat aatgaaaagt
    32821 catatacaaa atatttatta acagaaggta tagaaaagat tacaaaaaaa tacggtgaag
    32881 aagcttttga agtcgtaatt gaagcaatta aaggtgacaa aaaagcattt gtaagtgaag
    32941 tagcagatga actttatcat ttatttgtct tgatgcatgc gcttggcgtc gatttttcag
    33001 aaattgaggc ggaattagcg cgtagacatc ataagcgcaa taactttaaa ggtgaacgac
    33061 aaaatatcga acagtggtaa agcaagtatg gactaagata taaggaaaag gatcatggct
    33121 tatacactta caaatattgt ggaaaacgtg acattttcaa gtttaaaata cgacaccaac
    33181 atattttaac tatgaatgct gtgatggtac taaagttgcg aactcgttat agataagtag
    33241 tggataatca caatacgaaa tcaaaaataa ttataaaaag taaattgagc aactcaggaa
    33301 tagatgtcac tgttaaagat gtcgaaaagt atatgaatcg atataatgaa gttatgaagg
    33361 gaaaaaatgg cgaaaaagct aaagagttat gtttgtcgtt actacctatt aatatcatag
    33421 ttgtctttac attctttgta tttatactat aaatacaaat atatctagcc tgaaatagaa
    33481 atgtcatagc ctatttaaaa gacaatctcc attagaacta agatatgcat cccgaaagtt
    33541 agactaaaaa actaacttta tgggatgtat ttttatgcta atcatcataa attcgagatt
    33601 aagttaaagg tagttcaaga gtaattaaac aataaattaa aaatagtagg atacttactt
    33661 tgagggaaga aaattaactg tatatattta gtttaggaac aagtattacg gtttatcctg
    33721 atacaattat tgtggatggg atgatatttt taggtttaaa atacgacacc agcaaacata
    33781 ataactgtaa tagctcataa atctccccat atagctaatc taaaaaaata atacatcatt
    33841 ggaattaagc cccaagcatg taaatattaa aaatcaaaaa agatatatgt aaaaaagtt
//
LOCUS       DALAGD010000019        33416 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.019, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000019 DALAGD010000000
VERSION     DALAGD010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 33416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33416
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.019"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(50..1462)
                     /gene="atpD"
                     /locus_tag="O2701_000703"
     CDS             complement(50..1462)
                     /gene="atpD"
                     /locus_tag="O2701_000703"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCZ4292609.1"
                     /translation="MGIGRVTQVMGPVIDVRFEHNEVPKINNALVIDVPKEEGTIQLT
                     LEVALQLGDDVVRTIAMDSTDGVQRGMDVKDTGKEISVPVGDETLGRVFNVLGETIDL
                     KEEISDSVRRDPIHRQAPAFDELSTEVQILETGIKVVDLLAPYIKGGKIGLFGGAGVG
                     KTVLIQELINNIAQEHGGISVFAGVGERTREGNDLYFEMSDSGVIKKTAMVFGQMNEP
                     PGARMRVALSGLTMAEYFRDEQGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTTKGSVTSIQAVFVPADDYTDPAPATAFAHLDATTNLERKLTE
                     MGIYPAVDPLASTSRALEPSIVGQEHYEVARDVQSTLQKYRELQDIIAILGMDELSDE
                     DKQTVERARRIQFFLSQNFHVAEQFTGQKGSYVPVKTTVANFKDILDGKYDHIPEDAF
                     RLVGSMDDVIAKAKDMGVEV"
     gene            complement(1484..2350)
                     /locus_tag="O2701_000704"
     CDS             complement(1484..2350)
                     /locus_tag="O2701_000704"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCZ4292610.1"
                     /translation="MASLKEIDTRIKSTKKMKQITKAMNMVSSSKLRRAEKNTKQFTP
                     YMDKMQDAITAVAGASSNTNHPMLRPRKITRSGYLVITSDKGLAGAYSANVLKKLITD
                     IEAKHQDSSEYSIVVLGQQGVDFLKNRGYDIEYSQVDVPDQPSFKSVQALANHAIDLY
                     SEEEIDELNIYYSHYVSVLENKPTSRQVLPLSQEDSSKGHGHLSSYEFEPDKESILSV
                     ILPQYVESLIYGTILDAKASEHATRMTAMKNATDNATELIDDLSLEYNRARQAEITQQ
                     ITEIVGGSAALE"
     gene            complement(2381..3889)
                     /locus_tag="O2701_000705"
     CDS             complement(2381..3889)
                     /locus_tag="O2701_000705"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCZ4292611.1"
                     /translation="MAIKAEEISALLRSQIENYESEMSVTDVGTVLQIGDGIALIHGL
                     NDVMAGELVEFHNGVLGLAQNLEESNVGVVILGPYTGITEGDEVKRTGRIMEVPVGEE
                     LIGRVVNPLGQPIDGQGPINTTKTRPVEKKATGVMDRKSVDEPLQTGIKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQGTICIYVAIGQKDSTVRANVEKLRQAGAL
                     DYTIVVAASASEPSPLLYIAPYSGVTMGEEFMFNGKHVLIVYDDLTKQAAAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLNDDLGGGSITALPIIETQAGDISAYVPTNV
                     ISITDGQIFLQSDLFFSGVRPAINAGQSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     ESFAQFGSDLDEFTASKLERGKRTVEVLKQDQNKPLPVEHQVLIIYALTKGYLDDIPV
                     VDITRFEDELNHWAESNATELLNEIRETGGLPDAEKFDTAINEFKKSFSKSE"
     gene            complement(3911..4450)
                     /locus_tag="O2701_000706"
     CDS             complement(3911..4450)
                     /locus_tag="O2701_000706"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCZ4292612.1"
                     /translation="MVKVANKYAKALFDVSLDTNNLETINEELTVINEAVKDKIEQLR
                     MVDSNPTQTAEQRRELINGVFTDINPYIKNMMYVLADNRHISLIADVFKAFQSLYNGH
                     YNQDFATIESTYELSQEELDKIVKLVTQQTKLSKVIVDTKINPDLIGGFRVKVGTTVL
                     DGSVRNDLVQLQRKFRRVN"
     gene            complement(4450..4971)
                     /locus_tag="O2701_000707"
     CDS             complement(4450..4971)
                     /locus_tag="O2701_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCZ4292613.1"
                     /translation="MTETANLFVLGAAGGVEWGTVIVQVLTFIVLLALLKKFAWGPLK
                     DVMDKRERDINRDIDDAEQAKLNAQKLEEENKQKLKETQEEVQKILEDAKVQARQQQE
                     QIIHEANVRANGMIETAQSEINSQKERAIADINNQVSELSVLIASKVLRKEISEQDQK
                     ALVDKYLKEAGDK"
     gene            complement(5169..5381)
                     /gene="atpE"
                     /locus_tag="O2701_000708"
     CDS             complement(5169..5381)
                     /gene="atpE"
                     /locus_tag="O2701_000708"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCZ4292614.1"
                     /translation="MNLIAAAIAIGLSALGAGIGNGLIVSRTVEGVARQPEARGQLMG
                     IMFIGVGLVEALPIIGVVIAFMTFAG"
     gene            complement(5424..6152)
                     /gene="atpB"
                     /locus_tag="O2701_000709"
     CDS             complement(5424..6152)
                     /gene="atpB"
                     /locus_tag="O2701_000709"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCZ4292615.1"
                     /translation="MDHKSPLVSWNLFGFDIVFNLSSILMILVTAFLVFLLAIICTRN
                     LKKRPTGKQNFVEWIFDFVRGIIEGNMAWKKGGQFHFLAVTLILYIFIANMLGLPFSI
                     VTKDHTLWWKSPTADATVTLTLSTTIILLTHFYGIKMRGTKQYLKGYVQPFWPLAIIN
                     VFEEFTSTLTLGLRLYGNIFAGEILLTLLAGLFFNEPAWGWIISIPGLIVWQAFSIFV
                     GTIQAYIFIMLSMVYMSHKVADEH"
     gene            complement(6173..6625)
                     /locus_tag="O2701_000710"
     CDS             complement(6173..6625)
                     /locus_tag="O2701_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HCZ4292616.1"
                     /translation="MNRIDTTNRLYYHLVCMSGTVFMIDISHTEAYKMSRFYNIFKQF
                     IQYYFYLIIILGGLYLYTHHAFILGLIIGVTGSAINTCIFECYLAKAKRPDTMHISTG
                     NMWRYLVAIIACMIWYLNKAHVSIIGIIIGLMISYVVVIIRPLLKVSK"
     gene            complement(6693..7820)
                     /gene="wecB"
                     /locus_tag="O2701_000711"
     CDS             complement(6693..7820)
                     /gene="wecB"
                     /locus_tag="O2701_000711"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCZ4292617.1"
                     /translation="MKKIMTIFGTRPEAIKMAPLVKALEQEKMLEPIVVVTAQHREML
                     DSVLSTFEIKPKYDLNIMKSGQTLSEITSKSITQLEQVIQLEKPDMVLVHGDTMTTFA
                     GGLAAFYNQVPIGHVEAGLRSYDKYSPFPEEVNRQLVGVLADLHFAPTKNAASHLLSE
                     GKYSESVVVTGNTAIDAMKYTVDDNYKSNIMDKYHDKKFILMTAHRRENIGKPMENIF
                     KAVRRLIDEYTDLALVYPMHKNPKVREVAQKILGSHDRIELIEPLDVVDFHNFAKKSY
                     FILTDSGGIQEEAPSFNKPVLVLRSVTERPEGVEAGTLKVIGTNKQNVYQAAKELIDD
                     ERLYHQMSEASNPYGDGFASERIVNHIKYYLNLITEKPSDF"
     gene            complement(7844..8473)
                     /gene="upp"
                     /locus_tag="O2701_000712"
     CDS             complement(7844..8473)
                     /gene="upp"
                     /locus_tag="O2701_000712"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCZ4292618.1"
                     /translation="MSKVHVFDHPLIQHKLSYIRDVNTGTKEFRELVDEVGMLMAYEV
                     TRDLELQDVDIETPVTKMTAKRLAGKKLAIVPILRAGLGMTDGILSLVPAARVGHIGL
                     YRDPETLKAVEYFAKLPQDITERQIIVVDPMLATGASAIEAITSLKKRGAKNIRFMCL
                     IAAPEGVEKMHEAHPDVDIYIAALDEKLNDKAYITPGLGDAGDRLFGTK"
     gene            complement(8501..9739)
                     /locus_tag="O2701_000713"
     CDS             complement(8501..9739)
                     /locus_tag="O2701_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCZ4292619.1"
                     /translation="MSYITKQDKVIAEAIEREFQRQNSNIELIASENFVSEAVMEAQG
                     SVLTNKYAEGYPGRRYYGGCEFVDVTESIAIDRAKALFGAEHVNVQPHSGSQANMAVY
                     LVALGMGDTVLGMNLSHGGHLTHGAPVNFSGKFYNFVEYGVDKDTERINYDEVRKLAL
                     EHKPKLIVAGASAYSRTIDFKKFKEIADEVNAKLMVDMAHIAGLVAAGLHPNPVEYAD
                     FVTTTTHKTLRGPRGGMILCKEEYKKDIDKTIFPGIQGGPLEHVIAAKAVAFGEALEN
                     NFKTYQQQVVKNAKVLAEALINEGFRIVSGGTDNHLVAVDVKGSIGLTGKEAEETLDS
                     VGITCNKNTIPFDQEKPFVTSGIRLGTPAATTRGFDEKAFEEVAKIISLALKNSKDEE
                     KLQQAKERVAKLTAEYPLYQ"
     gene            complement(9766..10290)
                     /locus_tag="O2701_000714"
     CDS             complement(9766..10290)
                     /locus_tag="O2701_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HCZ4292620.1"
                     /translation="MKDLTMLLDELKDMSFFNKGDICLIGCSTSEVIGEKIGTVGSME
                     VAETIFNALDVVSKETGVTFAFQGCEHINRAITIEKSQYNPLTMEEVSVVPDVHAGGS
                     LATYAFQHMKDPIVVEHITVPCGIDIGQTLIGMHIKHVCVPVRTSVKQVGQAIVTIAT
                     SRPKKIGGERAKYQ"
     gene            complement(10397..10816)
                     /locus_tag="O2701_000715"
     CDS             complement(10397..10816)
                     /locus_tag="O2701_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HCZ4292621.1"
                     /translation="MKILFVCTGNTCRSPLAESIAKEVMPNHQFESRGIFAVNNQGVS
                     NYVEDLVEEHHLAETTLSQQFTEADLKADIILTMSYSHKELIEAHFGLQNHVFTLHEY
                     VKEAGEVIDPYGGTKEMYVHTYEELVSLILKLKDIIC"
     gene            complement(10813..11859)
                     /locus_tag="O2701_000716"
     CDS             complement(10813..11859)
                     /locus_tag="O2701_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCZ4292622.1"
                     /translation="MDTKIWDVREYNEDLQQYPKINEIKDIVLNGGLIGLPTETVYGL
                     AANATDEEAVAKIYEAKGRPSDNPLIVHIHSKGQLKDFTYTLDPRVEKLMQAFWPGPI
                     SFILPLKLGYLCRKVSGGLSSVAVRMPSHSVGRQLLQIINEPLAAPSANLSGRPSPTT
                     FNHVYQDLNGRIDGIVQAEQSEEGLESTVLDCTSFPYKIARPGSITAAMITEILPNSI
                     AHADYNDTEQPIAPGMKYKHYSPNTPLTIITDIESKIGNDGKDWSSIAFIVPSNKVAF
                     IPSEAQFIQLCQDDNDVKQASHNLYDVLHSLDENENISAAYIYGFELNDNTEAIMNRM
                     LKAAGNHIIKGCEL"
     gene            complement(11943..12779)
                     /gene="prmC"
                     /locus_tag="O2701_000717"
     CDS             complement(11943..12779)
                     /gene="prmC"
                     /locus_tag="O2701_000717"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCZ4292623.1"
                     /translation="MVNYKEKLDEAIHLTQQKGFEQTRAEWLMLDVFQWTRTDFVVHM
                     HDDMPKAMIMKFDLALQRMLLGEPIQYIVGFASFYGRTFDVNSNCLIPRPETEEVMLH
                     FLQQLEDDATIVDIGTGSGVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQI
                     QFLTGDALKPLINEGIKLNGLISNPPYIDEKDMVTMSPTVTRFEPHQALFADNHGYAI
                     YESIIEDLPHVMEKGSPVVFEIGYNQGEALKSIILNKFPDKKIDIIKDINGHDRIVSF
                     KW"
     gene            complement(12766..13842)
                     /gene="prfA"
                     /locus_tag="O2701_000718"
     CDS             complement(12766..13842)
                     /gene="prfA"
                     /locus_tag="O2701_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCZ4292624.1"
                     /translation="MFDQLDIVEERYEQLNELLSDPDVVNDSDKLRKYSKEQADLQKT
                     VDVYRNYKAKKEELADIEEMLSETDDKEEVEMLKEESNGIKAELPNLEEELKILLIPK
                     DPNDDKDVIVEIRAAAGGDEAAIFAGDLMRMYSKYAESQGFKTEIVEASESDHGGYKE
                     ISFSVSGNGAYSKLKFENGAHRVQRVPETESGGRIHTSTATVAVLPEVEDVEIEIRNE
                     DLKIDTYRSSGAGGQHVNTTDSAVRITHLPTGVIATSSEKSQIQNREKAMKVLKARLY
                     DMKVQEEQQKYASQRKSAVGTGDRSERIRTYNYPQSRVTDHRIGLTLQKLGQIMEGHL
                     EEIIDALTLSEQTDKLKELNNGEL"
     gene            complement(13843..14442)
                     /locus_tag="O2701_000719"
     CDS             complement(13843..14442)
                     /locus_tag="O2701_000719"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCZ4292625.1"
                     /translation="MYETYHSGWIECITGSMFSGKSEELIRRLRRGIYAKQKVVVFKP
                     AIDDRYHKEKVVSHNGNAIEAINISKASEIMTHDLTNVDVIGIDEVQFFDDEIVSIVE
                     KLSADGHRVIVAGLDMDFRGEPFEPMPKLMAVSEQVTKLQAVCAVCGSSSSRTQRLIN
                     GKPAKIDDPIILVGANESYEPRCRAHHIVAPSDNNKEEL"
     gene            complement(14789..15043)
                     /locus_tag="O2701_000720"
     CDS             complement(14789..15043)
                     /locus_tag="O2701_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCZ4292626.1"
                     /translation="MKQGIHPEYHQVIFLDTTTNFKFLSGSTKTSSEMMEWEDGKEYP
                     VIRLDISSDSHPFYTGRQKFAAADGRVERFNKKFGLKSNN"
     gene            complement(15161..16477)
                     /gene="rho"
                     /locus_tag="O2701_000721"
     CDS             complement(15161..16477)
                     /gene="rho"
                     /locus_tag="O2701_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCZ4292627.1"
                     /translation="MPERERTSPQYESFHELYKNYTTKELTQKAKTLKLTNYSKLNKK
                     ELVLAIMEAQMEKDGNYYMEGILDDIQPDGYGFLRTVNYSKGEKDIYISASQIRRFEI
                     KRGDKVTGKVRKPKDNEKYYGLLQVDFVNDHNAEEVKKRPHFQALTPLYPDERIKLET
                     ETQNYSTRIMDLVTPIGLGQRGLIVAPPKAGKTSLLKEIANAISTNKPDAKLFILLVG
                     ERPEEVTDLERSVEAAEVVHSTFDEPPEHHVKVAELLLERAKRLVEIGEDVIILMDSI
                     TRLARAYNLVIPPSGRTLSGGLDPASLHKPKAFFGAARNIEAGGSLTILATALVDTGS
                     RMDDMIYEEFKGTGNMELHLDRKLSERRIFPAIDIGRSSTRKEELLISKSELDTLWQL
                     RNLFTDSTDFTERFIRKLKRSKNNEDFFKQLQKSAEESTKTGRPII"
     gene            complement(16724..18151)
                     /locus_tag="O2701_000722"
     CDS             complement(16724..18151)
                     /locus_tag="O2701_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCZ4292628.1"
                     /translation="MRDYTKQYINGEWVESNSNETIEVINPATEEVIGKVAKGNKADV
                     DKAVEAADDVYLEFRHTSVKERQALLDKIVKEYENRKDDIVQAITDELGAPLSLSERV
                     HYQMGLNHFVAARDALDNYEFEERRGDDLVVKEAIGVSGLITPWNFPTNQTSLKLAAA
                     FAAGSPVVLKPSEETPFAAVILAEIFDKVGVPKGVFNLVNGDGAGVGNPLSEHPKVRM
                     MSFTGSGPTGSKIMEKAAKDFKKVSLELGGKSPYIVLDDVDIKEAAKATTGKVVNNTG
                     QVCTAGTRVLVPNKIKDAFLAELKEQFSQVRVGNPREDGTQVGPIISKKQFDQVQNYI
                     NKGIEEGAELFYGGPGKPEGLEKGYFARPTIFINVDNQMTIAQEEIFGPVMSVITYND
                     LDEAIQIANDTKYGLAGYVIGKDKETLHKVARSIEAGTVEINEAGRKPDLPFGGYKQS
                     GLGREWGDYGIEEFLEVKSIAGYFK"
     gene            complement(18389..18724)
                     /locus_tag="O2701_000723"
     CDS             complement(18389..18724)
                     /locus_tag="O2701_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ4292629.1"
                     /translation="MEVCPYLEETFKILGRSWNGLIINYLSRCNDCSAHFSDMKRDLK
                     TITPRALSLKLSELAQWELVEKQIISTSPVQIIYVLTEKGKALAEALHPIEAWAQSYV
                     DLTDQRTAK"
     gene            complement(18813..20072)
                     /locus_tag="O2701_000724"
     CDS             complement(18813..20072)
                     /locus_tag="O2701_000724"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCZ4292630.1"
                     /translation="MAQEVIKIRGGRTLNGEVNISGAKNSAVAIIPATLLAQGHVKLE
                     GLPQISDVKTLVSLLEDLNIKASLNGTELEVDTTEIQNAALPNNKVESLRASYYMMGA
                     MLGRFKKCVIGLPGGCPLGPRPIDQHIKGFKALGAEIDESSTTSMKIEAKELKGAHIF
                     LDMVSVGATINIMLAAVYATGQTVIENAAKEPEVVDVANFLTSMGANIKGAGTSTIKI
                     NGVKELHGSEYQVIPDRIEAGTYMCIAAACGENVILNNIVPKHVETLTAKFSELGVNV
                     DVRDERIRINNNAPYQFVDIKTLVYPGFATDLQQPITPLLFMANGPSFVTDTIYPERF
                     KHVEELKRMGANIEVDEGTATIKPSTLHGAEVYASDLRAGACLIIAGLIAEGVTTIYN
                     VKHIYRGYTDIVEHLKALGADIWTETV"
     gene            complement(20538..21398)
                     /locus_tag="O2701_000725"
     CDS             complement(20538..21398)
                     /locus_tag="O2701_000725"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002468309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HCZ4292631.1"
                     /translation="MPLVSMKEMLIDAKENGYAVGQYNINNLEFTQAILEASQEENAP
                     VILGVSEGAARYMSGFYTIVKMVEGLMHDLNITIPVAIHLDHGSSFEKCKEAIDAGFT
                     SVMIDASHSPFEENVATTKKVVEYAHEKGVSVEAELGTVGGQEDDVVADGIIYADPKE
                     CQELVEKTGIDALAPALGSVHGPYKGEPKLGFKEMEEIGLSTGLPLVLHGGTGIPTKD
                     IQKAIPFGTAKINVNTENQIASAKAVRDVLNNDKEVYDPRKYLGPAREAIKETVKGKI
                     KEFGTSNRAK"
     gene            21616..22137
                     /locus_tag="O2701_000726"
     CDS             21616..22137
                     /locus_tag="O2701_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2529 domain-containing protein"
                     /protein_id="HCZ4292632.1"
                     /translation="MSKILNTQLIGIFNRLEKQSLEIQMAAQCLIQAIGGEGYVYVKG
                     YDDLQFFESFILHSDERLKSSRKLDAIKDFKEIDSTDRVLLFAPFYNDQVALDIQKLI
                     DLDIDVVLISNKPKTDDFPDHLVHFIDLSTPRPIVYTEDYDKIVQPHAMALNYVYYDI
                     YTQMIEMTRDLEL"
     gene            complement(22246..23856)
                     /locus_tag="O2701_000727"
     CDS             complement(22246..23856)
                     /locus_tag="O2701_000727"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HCZ4292633.1"
                     /translation="MTKFIFVTGGVVSSLGKGITASSLGRLLKDRGLNVTIQKFDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKFSNVTAGKVYSHVLKKE
                     RRGDYLGGTVQVIPHITNEIKERLLLAGESTNADVVITEIGGTTGDIESLPFIEAIRQ
                     IRSDLGRENVMYVHCTLLPYIKAAGEMKTKPTQHSVKELRGLGIQPDLIVVRTEYEMT
                     QDLKDKIALFCDINKESVIECRDADSLYEIPLQLSQQNMDDIVIKRLQLNAKYETQLD
                     EWKQLLDIVNNLDGKITIGLVGKYVSLQDAYLSVVESLKHAGYPFAKDIDIRWIDSSE
                     VTDENAAEYLADVDGILVPGGFGFRASEGKISAIKYARENNVPFFGICLGMQLATVEF
                     SRNVLGLEGAHSAELDPATPYPIIDLLPEQKDIEDLGGTLRLGLYPCSIKEGTLAQDV
                     YGKAEIEERHRHRYEFNNDYREQLEANGMVISGTSPDGRLVEMVEIPTNDFFIACQFH
                     PEFLSRPNRPHPIFKSFIEASLKYQQNK"
     gene            complement(24192..24722)
                     /locus_tag="O2701_000728"
     CDS             complement(24192..24722)
                     /locus_tag="O2701_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HCZ4292634.1"
                     /translation="MKIQDYTKQMVDEKSFIDMAYTLLNDKGETMNLYDIIDEFRALG
                     DYEYEEIENRVVQFYTDLNTDGRFLNVGENLWGLRDWYSVDDIEEKIAPTIQKFDILD
                     ADDEEDQNLKLLGEDEMDDDDDIPAQTDDQEELNDPEDEQVEEEINHSDIVIEEDEDE
                     LDEDEEVFEDEEDFND"
     gene            complement(24834..25694)
                     /locus_tag="O2701_000729"
     CDS             complement(24834..25694)
                     /locus_tag="O2701_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4292635.1"
                     /translation="MAKELCFEGITLKAFDEQYRSAINDFDLNERQQIYSSLPKEVID
                     DAINDADRIANVAINDKNEVVGFFVLHRYYQHEGYDTPENVVYIRSLSINEKYQGFGY
                     GTKIMMSLPQYVQGVFPDFNHLYLVVDAENDNAWNLYERAGFMHTATKEEGPIGKERL
                     YYLDLDSKHVSSLKLEEESRSEVTNVHIINLMIDGQKVGFIALEQIGERMNIAAIEVD
                     KSYRFNGIGSSALRQLPTYLRKNYDNLNVITMILFGENNDFKPLCLNSNFVEIEQTDD
                     YVVFEKYLNY"
     gene            26118..26921
                     /gene="coaW"
                     /locus_tag="O2701_000730"
     CDS             26118..26921
                     /gene="coaW"
                     /locus_tag="O2701_000730"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II pantothenate kinase"
                     /protein_id="HCZ4292636.1"
                     /translation="MKVGIDAGGTLIKIVQEQDNQRTFKTELTKNIDQVVEWLNQQQI
                     EKLCLTGGNAGVIAENINIPAQIFVEFDAASQGLGILLKEQGHDLADYIFANVGTGTS
                     LHYFDGQSQRRVGGIGTGGGMIQGLGYLLSQITDYKQLTDMAQHGDRNTIDLKVRHIY
                     KDTEPPIPGDLTAANFGHVLHHLDADFTPSNKLAAVIGVVGEVVTTMAITVAREFKTE
                     NIVYIGSSFHNNALLRKVVEDYTVLRGCKPYYVENGAFSGAIGALYLEK"
     gene            complement(27051..27722)
                     /locus_tag="O2701_000731"
     CDS             complement(27051..27722)
                     /locus_tag="O2701_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2750 domain-containing protein"
                     /protein_id="HCZ4292637.1"
                     /translation="MSYKAYPFFRDILINECIYFASKNKKLVRLNYKSEAYVGVWTEE
                     SVAVSFLTSRDIPFDKVVKMDVDRFATYELDELFDEQDHIIMNQTMEEEGHLLNVVAV
                     TQEVMTELDKIRIKEFVQDVAKYDEVYGLTKKGSKQFILISENDSDEKKPHIMPVWSI
                     KNRALKVRDEDFEECDLITIEGSVFGEWLDELRDDHKAVAIDLKTGVVGTIVSAQKLS
                     NELTF"
     gene            complement(28121..29314)
                     /locus_tag="O2701_000732"
     CDS             complement(28121..29314)
                     /locus_tag="O2701_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HCZ4292638.1"
                     /translation="MTNNGNEPNLTLSDLYDKDVVYTSRPSYISNPWLKPDEHQSNFL
                     TGRELLIANQLPVIVHEASATDKLHQLFQVIGKEVPNSIYTFNNQQSYENLIKQLAHK
                     ENKKIYFQYIHDETILNQQYYALDKTLFVALNNKARIPEWTNGKFLPKRKVVKIEQFE
                     NEIKNWEFPLVIKPGDDLPTAGGYGVMICYHDADLQKAITRIKEATAETNSLIIEQKI
                     EEKANYCVQFAYSESLGIQYLGAATQLTDKYGFYNGNENTTNVPEHVIEAGRQIMENG
                     VNQGFFGVAGFDLLVDEDDNVYAIDLNFRQNGSTSMLLLANELNSGYQKFYSYHSKGD
                     NTHFFNTILKYVKEGSLYPLSYYDGDWYGEDKVKSRFGCIWHGDSKETVLENERAFLA
                     ELEHY"
     gene            complement(29314..30498)
                     /locus_tag="O2701_000733"
     CDS             complement(29314..30498)
                     /locus_tag="O2701_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HCZ4292639.1"
                     /translation="MSEKQQILDYIETNKYSYIEISHRIHERPELGNEEIFASRTLID
                     RLKEHDFEIETEIAGHATGFIATYDSGLDGPAIGFLAEYDALPGLGHACGHNIIGTAS
                     VLGAIGLKQVIDQIGGKVVVLGCPAEEGGENGSAKASYVKAGVIDQIDIALMIHPGNE
                     TYKTIDTLAVDVLDVKFYGKSAHASENADEALNALDAMISYFNGVAQLRQHIKKDQRV
                     HGVILDGGKAANIIPDYTHARFYTRAMTRKELDILTEKVNQIARGAAIQTGCDYEFGR
                     IQNGVNEFIKTPKLDDLFAKYAEEVGEAVIDDDFGYGSTDTGNVSHVVPTIHPHIKIG
                     SRNLVGHTHRFREAAASVHGDEALIKGAKIMALMGLELITNQDVYQDIIEEHAHLKGN
                     GK"
     gene            30809..31279
                     /locus_tag="O2701_000734"
     CDS             30809..31279
                     /locus_tag="O2701_000734"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCZ4292640.1"
                     /translation="MTKMNVESFNLDHTKVVAPFIRLAGTMEGLNGDVIHKYDIRFKQ
                     PNKEHMDMPGLHSLEHLMAENIRNHSDKVVDLSPMGCQTGFYVSFINHDNYDDVLNIV
                     EATLNDVLNATEVPACNEVQCGWAASHSLEGAKTIAQAFLDKRNEWHDVFGTGK"
     gene            complement(31431..31781)
                     /locus_tag="O2701_000735"
     CDS             complement(31431..31781)
                     /locus_tag="O2701_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292641.1"
                     /translation="MNILRYLLGIGFSVIGVLHFTRERQFRNIIPKCLPLRKTAVLVT
                     GIFEILFGLALLIKRPSQCLKNTINLFLLAVFPANIYMAVKKLPLGDKQLPTWQLYLR
                     LPMQFVLMGLIKKL"
     gene            complement(31795..33096)
                     /locus_tag="O2701_000736"
     CDS             complement(31795..33096)
                     /locus_tag="O2701_000736"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HCZ4292642.1"
                     /translation="MRMIDIIEKKRDGHTLTTEEINFFIGGYVKGDIPDYQASSLAMA
                     IYFQDMNDDERAALTMAMVNSGDMIDLSDIKGVKVDKHSTGGVGDTTTLVLAPLVAAV
                     DVPVAKMSGRGLGHTGGTIDKLEAIDGFHVEIDEATFVKLVNENKVAVVGQSGNLTPA
                     DKKLYALRDVTGTVNSIPLIASSIMSKKIAAGADAIVLDVKTGSGAFMKTLEDAEALA
                     HAMVRIGNNVGRNTMAIISDMNQPLGRAIGNALELQEAIDTLKGQGPKDLTELVLTLG
                     SQMVVLANKAETLEEARALLIEAINSGAALEKFKTFIKNQGGDETVIDHPERLPQAQY
                     QIEYKAKKSGYVTELVSNDIGVASMMLGAGRLTKEDDIDLAVGIVLNKKIGDKVEEGE
                     SLLTIHSNRQDVDDVVKKLDSSITIADHVVSPTLIHKIITE"
BASE COUNT        10157 a         6614 c         4804 g        11841 t
ORIGIN      
        1 agtgacaata tctaggttta atgtattcat tatccattcc tcctaattgt tatacttcaa
       61 cacccatatc tttagctttt gcaataacat catccatgct accaactaaa cggaatgcat
      121 cttctggaat atggtcatat ttaccatcta agatatcttt aaagtttgca actgttgtct
      181 taacaggtac ataagaacct ttttgaccag taaattgttc cgctacgtgg aagttttgag
      241 ataagaagaa ttgaattcta cgtgcgcgtt caactgtttg tttatcttca tcagataatt
      301 cgtccatacc taagatagca atgatatctt gtaattcacg gtatttttga agtgttgatt
      361 gtacatcacg agctacttca taatgttctt gacctacaat tgatggttcc aatgctcttg
      421 atgtagacgc taatggatcc acggctggat aaatacccat ttcagttaat ttacgttcta
      481 agtttgtagt tgcatctaaa tgggcaaacg ctgtcgcagg cgctgggtca gtatagtcat
      541 cggcaggtac gaataccgct tgaatagaag taactgatcc ttttgttgta gacgtaatac
      601 gttcttgtaa ttgtcccatt tcagtagcaa gtgttggttg gtaacctact gcagaaggca
      661 tacgacctaa taatgcagat acctcagaac cagcttgtgt aaatctgaaa atgttatcga
      721 tgaataataa tacgtcttga ccttgttcgt cacggaaata ttcagccatt gttaaaccag
      781 ataatgcaac acgcatacgt gcaccaggtg gctcattcat ttgcccgaat accatggctg
      841 ttttcttaat tacaccactg tcactcattt cgaagtataa atcgttacct tcacgagtac
      901 gttcacctac accggcgaat acagaaatac caccgtgctc ttgagcgatg ttgttaatta
      961 attcttggat taatactgtt ttacctacac cggcaccacc gaacaatccg attttaccac
     1021 ctttaatata aggtgctagt aaatctacta ctttaatacc tgtttctaaa atttgaactt
     1081 ctgttgaaag ttcatcgaat gctggtgctt gacgatggat aggatcgcgg cgaacagaat
     1141 cactaatttc ttctttaagg tcaattgttt cacctagtac attaaataca cgacctaatg
     1201 tttcgtcacc aacaggtaca ctaatttctt tgcctgtatc ttttacatcc atgcctcttt
     1261 ggacaccatc agttgaatcc atcgcaattg tacgaacaac gtcgtcacct aattgcagcg
     1321 caacttctaa tgttagttgt attgtacctt cttctttagg cacatcaata accaaggcgt
     1381 tattaatttt aggaacttcg ttatgttcaa atcgaacatc aattacagga cccataactt
     1441 gagttacacg gccaattccc atgctatttt cctcctttaa atattattca agcgctgcgg
     1501 aaccaccaac aatttcagta atttgttgcg taatttctgc ttgtctcgct ctgttatatt
     1561 ctaatgataa gtcatcaata agttcagttg cattatcagt ggcatttttc atcgcagtca
     1621 tacgtgttgc atgctcactt gcttttgcgt ctaatattgt tccgtaaatc aaactctcaa
     1681 catattgagg caagattaca cttaagatag attctttatc tggctcaaat tcataagaag
     1741 acaaatgacc atgcccctta ctagaatcct cttgagataa tggtaatact tgtctagatg
     1801 taggcttgtt ttcaagaacg ctgacataat gactatagta tatatttaat tcatcaattt
     1861 cttcttcact gtataagtct atagcatggt tagctagtgc ttgaacagat ttgaaagaag
     1921 gttgatcagg tacgtctact tgagaatact caatgtcata acctctattt tttaggaaat
     1981 caacaccttg ttgccctaaa actacaatac tgtattcgct actatcttga tgtttcgctt
     2041 caatatcagt aatcaatttt ttaagcacgt ttgcactata tgcacctgct aaacctttat
     2101 cactcgtgat aactaaatat ccacttctag taattttacg aggtcttagc attggatggt
     2161 ttgtattgct acttgcccct gcaactgcag taattgcatc ttgcatttta tccatatatg
     2221 gtgtgaattg ttttgtattt ttttcagctc tacgaagttt tgaacttgat accatgttca
     2281 tcgctttcgt aatctgcttc atttttttgg ttgattttat tcgagtatct atttctttaa
     2341 gagaagccac tatctcacca ccttatacta aacttgttta ttattcagat ttgctaaagc
     2401 tttttttgaa ttcgttaatt gctgtgtcaa acttctcagc atctggtaag ccaccagttt
     2461 ctctgatttc atttaacagt tcagtagcat ttgattctgc ccagtggttt aactcgtctt
     2521 caaaacgtgt gatatctaca actggaatat catctaaata tccttttgtt aatgcataaa
     2581 taatcaacac ttggtgttcg actggtaatg gtttgttttg atcttgtttt aagacttcaa
     2641 cagtacgttt accacgttct aatttacttg cagtaaattc atcaaggtct gaaccgaatt
     2701 gtgcaaatga ttcaagttct ctgtatgacg ctaagtcaag acgtaacgta ccagcaactt
     2761 tcttcattgc tttaatttgt gcagatccac caacacgaga tacagattgt ccggcattaa
     2821 tcgctggtct tacacctgag aagaataaat cagattgtaa gaagatttgt ccatctgtaa
     2881 ttgaaataac gtttgttggt acataagctg aaatatcacc agcttgtgtt tcgataattg
     2941 gtaatgcagt aattgaaccg ccacctaagt catcgtttaa ttttgctgct ctttctaata
     3001 atctactatg taagtagaat acgtcacctg ggtatgcttc acgacctgga ggtctacgta
     3061 ataataatga taactcacga taagctgctg cttgtttagt taaatcatca taaacgatta
     3121 aaacatgttt accgttaaac atgaattctt cacccattgt tacacctgaa tatggtgcaa
     3181 tatataataa tggagaaggt tcagaagctg atgctgctac aacaatagtg tagtctaaag
     3241 cgcctgcttg tcttaacttt tcaacatttg ctcttactgt tgaatctttt tgaccaatag
     3301 caacatagat acaaatcgta ccttgatctt tttggttcaa aattgtgtca attgcaattg
     3361 ttgttttacc tgtttgacgg tcaccgatga ttaactcacg ttgacctcta ccaattggta
     3421 ctaaagcatc aattgctttg atacctgttt gtaatggctc atctactgat ttacgatcca
     3481 ttacaccagt agcttttttc tctactggac gtgttttagt tgtgttaatc ggtccttgtc
     3541 catcaatagg ttgtcctaat ggattaacaa ctcttccgat tagttcttca cctactggta
     3601 cttccatgat acgaccagta cgtttaactt cgtcaccttc agtaatacct gtgtatggtc
     3661 ctaaaataac cacacccacg tttgactctt caaggttttg ggctaaacca agtacgccgt
     3721 tatggaattc tactagctca ccagccataa cgtcatttaa tccgtgaatt aatgcaatac
     3781 catcaccaat ttgtaataca gtacctacat cagttacgga catttctgac tcataatttt
     3841 caatttgtga gcgaagtaat gcactgattt cttcagcttt tatggccatc tatgtcactc
     3901 ctctttataa ttaattaact cttctaaatt ttctttgtaa ttggacaaga tcatttctaa
     3961 cactaccatc taatacagtt gtgccgactt taactctaaa tccaccaatt aaatctggat
     4021 taatttttgt atctacaata actttagata acttcgtttg ttgagttact agtttgacaa
     4081 tcttatctaa ctcttcttga ctcaattcat atgttgactc aattgttgca aaatcttgat
     4141 tgtagtgtcc gttatataag ctttggaacg ccttgaatac gtcagctatt aatgagatat
     4201 gtctattatc tgctaacacg tacatcatat ttttaatgta tggattaatg tcagtaaata
     4261 caccattaat caactcacga cgttgttctg ctgtttgagt tggattactg tcaaccattc
     4321 tcaattgctc aattttatct tttactgctt catttataac tgttaattct tcattaatag
     4381 tctctaaatt atttgtatct aatgacacgt caaataatgc tttagcatac ttgttagcta
     4441 cttttaccat tatttatcgc ctgcctcttt tagatacttg tcaaccaatg ctttttggtc
     4501 ttgttcagaa atttcttttc taagaacttt agaagcaatt aacactgata gttcagatac
     4561 ttgattatta atatctgcaa tggcacgttc tttttggcta ttgatttcac tttgtgctgt
     4621 ttcaatcata ccgtttgcac gtacgtttgc ttcatgaata atttgttctt gctgttgacg
     4681 tgcttgaacc ttagcatctt ctaaaatctt ttgaacttct tcttgtgttt ctttaagttt
     4741 ttgtttattt tcttcttcaa gtttctgtgc atttaactta gcttgttctg cgtcatcgat
     4801 atctctgtta atatctcttt cacgtttatc cattacatct ttcaatggac cccatgcgaa
     4861 ctttttaagt aacgcaagta acacgatgaa agttaggacc tgtacaatca cagtacccca
     4921 ctcaacgcct ccagctgcac caagaacgaa taagttagct gtttcagtca cgggttcatg
     4981 acactccttt cattaaaata atcgctagtg ttatatagaa aaaaacgacg aggttaggac
     5041 ctactgaatt tatgcgatag gcatggtgcg ttcatatctc aactatttaa taaagttcaa
     5101 agatattact atatatgcat catgaattat gtcctatgcg ctttgtcccg acctctttta
     5161 tctgttaatt atccagcaaa tgtcatgaat gcaattacta caccgatgat aggtaatgcc
     5221 tcaactaaac ctacaccaat gaacatgata cccattaatt gaccacgtgc ttctggttga
     5281 cgtgctacac cttcaactgt tcttgaaacg attaaaccgt taccgatacc tgctcctaat
     5341 gctgataaac caattgcgat tgctgctgcg attaaattca taatttaatt tcctcctgtg
     5401 attgtatata attattgaaa tttttagtgt tcatctgcca ctttatgtga catataaacc
     5461 atcgaaagca taataaagat atatgcttgg attgttccta caaatattga aaatgcttgc
     5521 caaacaatta atcctgggat actaataatc caaccccatg ctggttcgtt aaagaataag
     5581 ccagcaagta atgttaatag tatctcacct gcaaatatgt taccgtacaa acgcagacca
     5641 agcgttaatg ttgaagtgaa ctcttcaaaa acattaataa ttgccaatgg ccaaaacggc
     5701 tgtacataac ctttaagata ttgtttcgta ccacgcattt taattccata aaagtgagtt
     5761 aacagtatta tcgttgtaga caacgttaaa gtcactgttg catcagctgt cggtgatttc
     5821 caccacaatg tgtgatcttt cgttactata gaaaacggaa gacctaacat attagctata
     5881 aaaatgtaca gaatcagcgt tactgctaag aagtggaatt gaccaccttt tttccaagcc
     5941 atgttacctt caatgattcc cctcacgaaa tcaaaaatcc attcaacgaa attttgtttg
     6001 ccagttggtc tttttttcaa attacgcgta caaatgatag caagtagaaa aacaagaaac
     6061 gccgtaacaa gtatcatcaa tatacttgat aaattgaaaa cgatatcaaa accgaataaa
     6121 ttccaactca cgagcgggga tttgtgatcc ataatctcac ctctttctta atttatttgc
     6181 tcacctttag taaaggacgt atgataacta caacatatga aatcattaaa ccaataatta
     6241 taccgatgat acttacatgc gctttattaa ggtaccaaat catacaggca ataattgcaa
     6301 ctaagtatcg ccacatattt ccagttgaaa tatgcatagt gtctggtctt ttagctttag
     6361 ctaaatagca ttcaaaaata catgtgttga ttgcagaacc agtaacacca atgattaatc
     6421 ctaagataaa tgcatggtgt gtataaaggt ataatcctcc caatattatt attagataaa
     6481 aataatattg aatgaactgt ttaaaaatgt tgtagaaacg actcatttta tatgcctccg
     6541 tgtgactgat atcaatcatg aaaaccgttc cactcataca aactaaatga taatataggc
     6601 gatttgtggt gtcaattcta ttcaaattgt tgtaaaaaaa taagcatagt tagacacaat
     6661 ttagacacaa ttagacaaaa agtctatttt cctcagaaat cgcttggttt ttcagtgatt
     6721 aaattcaaat aatacttaat atgattaact attctttctg aagcaaatcc atcaccgtaa
     6781 ggattagatg cttcagacat ttgatgatat aatctctcat catcgattaa ttcttttgca
     6841 gcttgataca cattttgctt atttgttcca atgactttta atgtgccagc ttcaacacct
     6901 tcaggacgtt ctgtaacact tcgcaaaact aaaactggct tattaaatga tggcgcttct
     6961 tcctgaattc cacctgaatc tgtcaaaata aaataagatt ttttagcaaa attatggaaa
     7021 tctactacgt ccaaaggttc aatcaattca attctgtcat gactacctaa aatcttttga
     7081 gccacctctc gaactttcgg gtttttatgc attggatata ccagtgctaa atcagtatac
     7141 tcatctatta agcgtctaac cgctttaaat atattttcca tgggtttccc gatattttct
     7201 cgtcggtgtg ctgtcataag aatgaatttt ttgtcatggt atttatccat gatgttagat
     7261 ttataattgt catcaactgt atatttcata gcatcaatcg cagtattacc agtgacaaca
     7321 acactttctg aatatttccc ttcacttaac aaatgcgatg cagcattttt agtaggtgca
     7381 aaatgtaagt cagctaatac accaactaat tgtctattca cctcttctgg aaaaggtgaa
     7441 tatttatcat aacttctaag ccctgcttca acgtgtccaa tcggcacttg gttataaaat
     7501 gccgctaaac cacctgcaaa tgtcgtcatc gtatcaccat gtacaagtac catgtctggt
     7561 ttttctaatt gaatcacttg ttctaattga gtgattgatt tagaagttat ctcagaaagt
     7621 gtctgtcctg atttcataat attcaaatcg tattttggtt tgatttcaaa ggtacttaat
     7681 actgaatcaa gcatttctct atgctgtgct gtaacaacaa caattggctc gagcattttt
     7741 tcttgttcca aagctttaac taaaggagcc atctttatgg cttcgggtct tgttccaaat
     7801 atggtcataa tctttttcat caaactactt atctccgatt cttctattta gtaccaaaca
     7861 atctatctcc agcgtcgcct aaccctggtg tgatatatgc tttgtcattt agcttttcat
     7921 caagtgcagc aatataaata tctacatctg gatgtgcttc atgcatcttt tctacgcctt
     7981 ctggtgctgc aattaaacac atgaagcgaa tatttttagc gccacgtttc ttcaatgaag
     8041 taatagcttc aattgctgat gcgcctgttg ctaacatagg atcaacaaca atgatttgtc
     8101 tttcagtaat atcttgaggt aacttagcaa aatactctac agcctttaat gtttcgggat
     8161 ctcgatataa accgatatgt ccaactctgg ctgcaggtac taaacttaaa ataccatcag
     8221 tcatacctaa accagctctt aaaattggaa cgatagctaa ttttttacca gctaatcgtt
     8281 tagccgtcat tttagttaca ggcgtttcaa tatcaacatc ctgaagctct aagtctctag
     8341 ttacttcata tgccatcaac ataccaactt cgtctacaag ttctctaaat tctttagtac
     8401 ctgtatttac atctctaata tagcttagtt tgtgttgaat taatggatga tcgaaaacgt
     8461 gtactttact cataaaaatt actcctatct ttgtgtatgt ttattgatat agaggatatt
     8521 cagctgttaa tttcgcaacg cgttctttag cttgttgtaa tttttcttca tctttactat
     8581 ttttcaatgc taaactgatg atttttgcaa cttcctcaaa agctttttca tcaaatccac
     8641 gcgttgttgc agcaggtgta cctaaacgta taccactcgt tacaaaaggt ttttcttgat
     8701 cgaacggaat ggtatttttg ttacatgtga taccaactga atctaaagtc tcttcagctt
     8761 ctttaccagt aagtcctata gaccctttta catcaacagc tactaagtga ttatctgtac
     8821 cgccagaaac aattctaaat ccttcattaa ttaatgcttc tgcaagaact tttgcgtttt
     8881 taaccacttg ttgttgatac gttttgaaat tattttctaa cgcttctcca aaagcaactg
     8941 cttttgctgc aataacatgc tcaagaggtc caccttgaat accagggaaa attgttttat
     9001 ctatgtcttt tttatattct tccttacata aaatcatacc accacgtggt ccgcgtaatg
     9061 ttttgtgtgt tgtagttgtt acaaaatcag catattctac tggatttgga tgtaaacctg
     9121 ccgctactaa tcctgcaata tgtgccatgt ctaccattaa cttagcgttt acttcatctg
     9181 cgatttcttt aaactttttg aagtcaattg ttcttgaata tgctgatgct cctgccacaa
     9241 taagcttagg cttatgctct aacgctaatt tacgaacttc atcataattg attcgttctg
     9301 tgtctttatc tactccatat tcaacgaaat tgtagaattt accactaaaa ttaacaggcg
     9361 ctccatgtgt caagtgacca ccatgactca aattcatacc taaaactgtg tcgcccattc
     9421 ctaatgcaac taagtaaaca gccatgttcg cttgtgaacc tgaatgtggt tgaacattga
     9481 catgttcagc tccaaacaat gctttagcac gatcaattgc gatgctttca gtaacatcta
     9541 caaactcaca gccaccataa tatcggcgtc ctggatagcc ttcagcatac ttattagtca
     9601 acactgaacc ttgtgcttcc ataaccgctt ccgatacaaa attttccgat gcgattaact
     9661 ctatgttgct attttgtctc tgaaattctc tctcgattgc ttctgcgata actttatctt
     9721 gcttggtgat ataagacata aaatctcccc ttctttcaaa aaaacttatt ggtatttagc
     9781 acgttcgcca ccaatctttt tcggcctaga tgtggcaata gttacaattg cctgtcctac
     9841 ttgctttact gaggtcctta caggtacaca tacatgctta atatgcatgc ctattaacgt
     9901 ttgaccaata tcaattccac aaggaacagt aatatgttcg accacgatcg gatccttcat
     9961 atgctgaaaa gcgtatgttg ccaaactccc tccagcatgt acatctggaa cgacggaaac
    10021 ttcttccatt gttaatggat tatactgaga tttttctatt gttatcgctc tgttgatatg
    10081 ttcacatcct tgaaaagcaa aagtaacgcc tgtctcttta ctcacaacat ctaatgcatt
    10141 aaaaatagtt tctgcaactt ccatcgaacc gacagtccct attttttcgc caatgacttc
    10201 cgatgttgaa catccaatta aacatatatc tcctttatta aaaaaggaca tatcttttaa
    10261 ttcgtctaat aacattgtca aatctttcat aaaagcccac ccttcctaaa aataaaaaag
    10321 gaatatagca aagtgctaca ctcctctatt ataacttatt taactgttaa catatactaa
    10381 ttatacagaa ttcctactag caaataatat cttttaattt taaaattaaa cttacaagtt
    10441 cttcataggt atgtacatac atttcttttg ttccaccgta tggatctata acttctcctg
    10501 cttcttttac atattcatgc aatgtgaaaa catgattttg caaaccaaag tgtgcctcta
    10561 ttaattcttt gtgcgaatac gacatcgtca aaataatatc tgctttcaaa tctgcttcag
    10621 taaattgttg cgataaggtc gtttcagcta aatgatgttc ttcaactaag tcttcaacat
    10681 aattcgaaac accttgattg ttcacagcga atatacctct tgattcaaat tgatgatttg
    10741 gcataacctc ttttgcaata ctttccgcta atgggctacg acatgtgtta cctgtacaaa
    10801 cgaataaaat cttcatagtt cacatccttt aataatgtga ttacctgcag cttttaacat
    10861 gcgattcata attgcttctg tattatcatt cagctcaaag ccgtatatat acgccgctga
    10921 aatattttca ttttcatcaa gtgaatgtaa cacatcataa agattatgac ttgcttgttt
    10981 aacatcattg tcatcctgac ataattgaat gaattgcgct tcacttggta taaacgccac
    11041 cttattactc ggcacaataa aagctataga agaccaatct ttaccgtcat ttccaatttt
    11101 gctctcaata tctgtaataa ttgtaagtgg tgtattgggt gagtaatgct tatacttcat
    11161 acctggtgca attggctgtt cagtatcatt ataatcagca tgggcgatac tattcggaag
    11221 tatttctgta atcattgctg ctgttataga accaggtctt gcaattttat aaggaaaaga
    11281 tgtgcaatct aaaaccgtac tttctaatcc ttcttcactt tgttcagctt gaacaatacc
    11341 atcgatacgg ccattcaaat cttgatatac atgattgaaa gttgttggtg aaggtctacc
    11401 acttaaatta gcacttggag cagctagagg ttcatttatg atttgtaata attgtctacc
    11461 tacagaatgg cttggcattc taacagcaac tgatgataaa cctccagaaa cttttcgaca
    11521 tagatagcct agctttaacg gcaatataaa cgaaataggg cccggccaga atgcctgcat
    11581 taacttttct acgcgtggat ccaaagtata tgtaaaatct tttaattgac ctttactgtg
    11641 tatatgaaca ataagcggat tgtcagatgg acggccttta gcttcatata ttttagctac
    11701 agcttcttca tctgtcgcat ttgctgcaag tccataaact gtttcagttg gtaaacctat
    11761 taaaccaccg tttaaaacaa tgtcttttat ttcattaatt ttaggatatt gctgtaaatc
    11821 ttcattatat tctctaacat cccaaatttt agtatccaac ttaatcacgc ctttcttatt
    11881 tatcataata taaagcaaaa agctatgcac ttaactaatc atagcaaagg cataacttct
    11941 aattaccatt taaatgagac gattcgatcg tggccattta tatctttaat aatgtcgatt
    12001 tttttgtcag gaaatttatt taaaattatt gatttaagtg cctcaccttg attgtaacca
    12061 atttcaaaaa caactgggct gcctttttcc ataacgtgag gtaaatcttc aatgattgat
    12121 tcataaatag catatccatg gttatctgca aacaatgcct gatgtggttc gaatctcgta
    12181 accgttggag acatcgtaac catatctttt tcatctatat atggtggatt agatatcaag
    12241 ccgttcaact tgataccttc attaattaag ggctttaatg catcccctgt taaaaattgt
    12301 atttgtgatt gatgcttctc agcattatta cgagccatat tcattgcttc aagtgaaata
    12361 tcagtagcaa taacatttaa atccggcttt tcacatttca aagtaattgc aagtacacca
    12421 ctacccgttc cgatatctac gattgttgca tcatcttcta actgttgtaa gaaatgcaac
    12481 attacttctt cagtttcagg tcttggtatc aaacaatttg agtttacatc aaacgttcta
    12541 ccataaaatg aggcaaagcc aactatatac tgtataggct ctcctaataa catacgttgt
    12601 aatgctaagt cgaacttcat aatcatcgct ttcggcatat catcatgcat gtggactaca
    12661 aagtccgtac gcgtccattg aaatacatct aacattaacc attcagctcg tgtttgttca
    12721 aacccttttt gttgtgttaa atgaattgct tcatctaact tttctttata attcaccatt
    12781 attaagttct ttcaatttat ctgtctgctc tgataaagtc agtgcatcta taatttcttc
    12841 taaatggcct tccataattt gccctaattt ttgaagcgtt agacctatac gatggtctgt
    12901 tacacggctt tgtggataat tataagttcg aatacgttct gaacgatcac cagtaccgac
    12961 tgctgattta cgttgtgacg catacttttg ttgttcttct tgaactttca tatcgtataa
    13021 acgtgctttt aacactttca ttgctttttc acggttttga atttgagact tctcagaaga
    13081 tgttgcaatg acaccagttg gtaaatgggt aatacgtact gcagagtcag ttgtgtttac
    13141 gtgctgacca cctgcaccac ttgaacgata cgtgtcgatt tttaaatctt catttctaat
    13201 ttcaatttct acatcttcaa cttctggtaa aactgccact gtagctgttg aagtatgaat
    13261 acgtccacct gattctgttt caggcacacg ttgaacgcgg tgcgcaccat tttcaaattt
    13321 caatttacta tacgcgccat taccagaaac tgagaaacta atttctttgt aaccaccatg
    13381 gtcactttca gacgcttcta ctatttcagt tttgaatcct tgtgattcag catactttga
    13441 atacatacgc attaaatcac cagcaaaaat cgcagcctca tcaccacctg ctgctgctct
    13501 tatttctaca ataacgtctt tgtcatcatt aggatcttta ggaatcaata atattttaag
    13561 ctcttcttca agatttggaa gttcagcttt aataccatta ctctcctctt ttaacatttc
    13621 tacttcttct ttatcatcag tctcacttaa catttcttca atatcagcta attcttcttt
    13681 tttagcttta tagttacgat aaacatctac agttttttgt aaatcagctt gctctttaga
    13741 atatttacgt aatttatctg aatcatttac aacatctggg tcacttaaca gttcatttaa
    13801 ctgttcgtat ctttcttcta caatatctaa ttgatcaaac acttataatt cctccttatt
    13861 attatcacta ggtgctacga tatggtgcgc gcgacaacgt ggctcataac tttcattggc
    13921 acctactaag ataatcggat catcgatttt agctggttta ccatttatta atcgttgcgt
    13981 tctactagat gaagaaccac aaacagcaca aactgcttga agtttcgtta cttgttcact
    14041 gacagccatc aatttaggca ttggttcgaa cggttcgccc ctaaaatcca tatctaatcc
    14101 agcaacaata acacggtgtc catctgctga tagtttttct actatactta caatttcatc
    14161 gtcaaaaaat tgcacttcgt ctattcctat aacatcaaca ttagttaagt cgtgcgtcat
    14221 aatttcactt gctttagaaa tattaatcgc ttcaatggca ttaccattat gagagaccac
    14281 tttttcttta tgatatcgat catcaatcgc cggtttaaat acaacgactt tttgtttagc
    14341 gtatataccc cttcttagac gtcttattag ttcttcggat ttaccgctaa acatactacc
    14401 tgtaatacat tctatccaac cggaatggta agtttcatac attgagagtt ccaccttttt
    14461 caaaacataa tcgctttatt atatcatatt tcaaatattc ataaatgtct ttttcataat
    14521 tatatcgata ttgtacatga acaattattt tataaatttg tcttgcatat ttatatacag
    14581 ctattctaat ttgttgcttc ttattcgtgc gatgtttcta ctcaatacaa actttaataa
    14641 agggggaaat ttatcattat gtattaacaa catggtattg agtcacttct ttgaacacag
    14701 tcgtaagtat atacccttac tacgcttata aataaaaaag ctgtctacaa ttccattata
    14761 ggatgcagac agcttaacga ttactatatt agttgtttga tttaagacca aactttttgt
    14821 tgaaacgttc cacacgtcca tccgcagcag cgaatttttg acgtccagtg tagaatggat
    14881 gtgaatcaga tgaaatatct aaacgaataa ctgggtattc ttttccatct tcccactcca
    14941 tcatttctga agatgttttt gtagaaccgc ttaagaattt gaagtttgta gtagtatcta
    15001 aaaagattac ttggtgatac tcaggatgaa ttccttgttt cattattttc agctcctttg
    15061 ccctgaacca tctggaacag agttatttgt gagtttttac ccaatactgt gtaattataa
    15121 aggtaattaa ttcaaaacgc aagcccctat ataatgttta ttaaattata ggtcgacccg
    15181 ttttagtact ttcttctgca gacttttgta gctgcttgaa gaaatcttca ttattcttag
    15241 accttttaag tttgcgaata aatctttcag taaagtcagt tgagtcagtg aatagatttc
    15301 ttaattgcca taatgtgtct aattcagatt tacttatcaa caattcttct ttacgcgttg
    15361 aacttctgcc aatatcaatt gcagggaaga tacgacgttc agacaattta cgatctaaat
    15421 gtaactccat gttacctgtt cctttaaatt cttcgtaaat catatcgtcc atacgtgaac
    15481 ccgtatcaac taatgcagtt gcaagtattg ttaaacttcc acccgcttca atatttctcg
    15541 ctgcaccgaa gaatgctttt ggtttgtgta aagatgcagg atctaaacca cctgataatg
    15601 tacgaccact tggtggaata actaagttat aagcgcgtgc taatctcgtt atagaatcca
    15661 ttaaaataat gacatcttcc ccaatttcta ctaaacgctt tgcacgttca agtaataatt
    15721 cagctacttt aacatggtgt tctggtggtt cgtcaaacgt tgaatgaacg acttcagcag
    15781 cttctactga gcgttctaaa tctgttacct cttcaggacg ctcgccaact aacaaaataa
    15841 atagctttgc atctggtttg ttcgtactga ttgcattcgc tatttctttt aataacgatg
    15901 ttttacctgc tttaggtggc gccactatta aaccacgttg acctaaacca atcggtgtta
    15961 ctaaatccat gatgcgcgtt gaataatttt gtgtttctgt ctctaattta atacgctcat
    16021 ctggataaag tggtgtcaaa gcttggaaat gcggacgttt cttcacttct tctgcgttat
    16081 ggtcattgac aaagtcaact tgtaataagc cataatattt ttcgttatct ttaggttttc
    16141 taactttccc agttacttta tccccacgtt taatttcaaa acgacgaatt tggctagcag
    16201 atatataaat atctttttcc cctttagaat agttcactgt tcttaaaaaa ccataaccat
    16261 ctggttgtat atcatctaag ataccttcca tataatagtt accatctttt tccatttgtg
    16321 cttccataat agctagaaca agttcttttt tatttaattt actatagttc gtcaacttaa
    16381 gagttttagc tttttgagtg agttccttgg tagtatagtt cttgtacaat tcgtggaatg
    16441 attcatactg aggagatgta cgttctcttt caggcatata gtttacaccc atttcattat
    16501 tttttagtta gtaagtaata gataggcatg acaatgcata aataactgtg ctagcacaat
    16561 cccattatgc taatccccaa ccttttaaat acatgaataa ctatagcaaa tttttatttt
    16621 aattacaaaa tctatttata aaaattgcaa aattaaaaca gtacacaaaa cttaattagt
    16681 catgtgcact gttttattgc gatttttaat tcaaaaatca aatttattta aaatatccag
    16741 ctatagattt cacttctaag aactcttcaa taccataatc gccccattca cgacctaaac
    16801 cagattgttt atagccacca aatggtaaat ctggctttct acctgcttcg tttatttcta
    16861 ctgtacctgc ttcaatagaa cgagctactt tatgcaatgt ttctttgtcc ttaccaataa
    16921 catatcctgc caaaccatat tttgtatcat ttgcaatttg aatcgcttca tctaaatcgt
    16981 tataagtgat aactgacatt actggcccaa aaatttcttc ttgtgctatc gtcatttgat
    17041 tatctacatt aataaaaatt gtcggacgtg caaagtatcc tttttcaagt ccttctggtt
    17101 taccaggacc accataaaat aattcagcac cttcttcaat acctttatta atataatttt
    17161 gtacttgatc aaattgtttt ttactaatga tagggcctac ttgtgtacca tcttctcttg
    17221 gattaccgac acgcacttgg ctaaattgtt cttttaattc agctaagaat gcatctttaa
    17281 ttttgttagg cactaaaaca cgtgtaccag ctgtacatac ttgaccagta ttattaacaa
    17341 ctttgcctgt tgttgcttta gccgcttctt taatatctac gtcatctagg acgatatatg
    17401 gtgatttgcc accaagctct aatgatacct ttttaaaatc tttagcggct ttttccataa
    17461 ttttagaacc agtagggcct gatcctgtaa atgacatcat gcgtacttta ggatgttcag
    17521 ataaaggatt cccaacacca gcaccatcac cattaacaag gttaaataca cctttaggaa
    17581 caccgacttt atcaaaaatc tcagctaaaa taacagctgc aaatggtgtt tcttcagatg
    17641 gtttaagtac aactggacta ccagccgcaa atgctgctgc taattttaat gatgtttggt
    17701 ttgtagggaa gttccacggt gtaattaatc cagatacacc gattgcttct ttaacaacta
    17761 aatcatctcc gcggcgttct tcaaattcgt agttatctaa tgcgtctctc gctgcaacaa
    17821 aatggtttag tcccatttga taatggacac gctcagataa tgataaagga gcacctaatt
    17881 catccgtaat agcttgtaca atatcgtctt ttctgttttc atattctttt acaattttat
    17941 ctaataacgc ttgtctttct ttcacagatg tatgacggaa ctctaaataa acatcgtctg
    18001 ccgcctcgac ggctttatca acatcagctt tattaccttt agcaactttc ccgattactt
    18061 cttcggttgc tggatttata acttctatcg tttcattact attactttct acccattcgc
    18121 cattaatgta ttgctttgtg tagtctctca ttattctttc actcctcaag aatgatttat
    18181 atattattta tccgtaatgc atacgatata aacattacat tccattatga tacgctccct
    18241 tttaatatgt tcaaaataaa atgctttaaa attttgctgc atctattttt ttatattttt
    18301 aaataaaaat aactattccc acatcatcgc aaataatgcg aagacttaga aatagttata
    18361 atcaatgcta tgtttaaacg tcatgtaatt atttagcagt acgttgatct gttaaatcga
    18421 catatgattg cgcccatgct tcaattggat gtaaagcctc tgctaacgct ttaccttttt
    18481 cagttagcac ataaataatt tgtactggac tcgtagaaat gatttgcttc tcaactaact
    18541 cccattgtgc aagctctgac aacttaagac ttaaagcacg tggtgttatt gttttcaaat
    18601 ctcttttcat atcggaaaag tgtgctgaac agtcattaca tcttgagaga taattaatga
    18661 ttaatccatt ccaacttcta ccaagtattt taaaagtttc ttcgagatac ggacatactt
    18721 ccatcatctt cacctctcat caataaattt agaatgtaaa cgaacaccat tttttaatca
    18781 gtataattat tataccacaa aacaagataa atctatacag tttccgtcca aatatctgca
    18841 cctaaagctt ttaagtgttc tacaatatct gtataacctc tataaatatg tttaacattg
    18901 taaattgtag ttacaccctc agcaattaaa ccagcaataa ttaaacaagc tcctgctctt
    18961 aaatcgctag cataaacttc agcaccatgt aatgttgatg gttttatcgt tgctgtgcct
    19021 tcgtcaactt caatatttgc acccatgcgc tttaattctt caacatgttt aaaacgctcc
    19081 ggataaatcg tatcagttac aaatgaagga ccatttgcca taaataataa tggtgtaata
    19141 ggctgttgca aatcagtagc aaaacctgga tatactagtg ttttaatatc aacaaattga
    19201 tatggcgcat tattattgat gcgaattctt tcgtctctta catcaacatt cacacctaat
    19261 tcactaaatt tagcagttaa tgtttctaca tgtttcggaa caatattatt taatataaca
    19321 ttttctccac atgctgcagc gatacacata tatgtgcctg cttcaattct atcaggtata
    19381 acttgatact cagaaccatg taattctttg acgccattga ttttaattgt tgatgtaccc
    19441 gctcccttaa tattagctcc catacttgtt aagaagttag caacatcaac tacttccggt
    19501 tctttagcag cattttcaat tacagtttgt cctgttgcat aaactgcagc tagcataatg
    19561 ttaattgttg cacctacgct aaccatatct agaaaaatat gtgcaccttt taactcttta
    19621 gcttcgattt tcattgatgt cgtacttgat tcatcaattt cagcacctaa agctttaaat
    19681 cctttaatat gttgatcaat tggacgtggc ccaagtggac atcctcccgg taaaccaatc
    19741 acacattttt taaatctacc taacatggca cccatcatat agtatgaagc acgtaatgat
    19801 tcaactttat tatttggtaa tgcagcattt tgtatttcag ttgtatcaac ttctaattcc
    19861 gtaccattta gtgatgcctt aatatttaaa tcttctaata aactcactaa agttttaaca
    19921 tcagagattt gcggtaaccc ttctaatttc acatgtcctt gagctaataa tgttgcagga
    19981 atgatagcta cagcactgtt tttcgcgcca ctgatattaa cttccccatt tagtgtgcgt
    20041 ccacctctta tttttattac ctcttgagcc atatcattta tctcctttgt ccattacttt
    20101 ttaatttttt gcattattag cattataaat taattttact ttatttgtaa aatagtttat
    20161 tttaatcgtt aaaatcttgt ctttctattt tcgattgatt aataacaaat cattcacctt
    20221 tactaatata tgctgttctt ccccacttga ccatggtcta aagtctgatt attcaactaa
    20281 atattgacgt tgttttgaca attatttagt tttaattcta cggttttcac gtaaactttt
    20341 ttcataaatt gctcattttc cttataatta tatcacaatc aattttgata tattttacca
    20401 tttttacgct ttcattttaa aatcaaactg ttttatttat tacttatacg acataaaaaa
    20461 gaagcaaaca ccattttgtc tgcttctttt aaaattaata tccctacgtt attaataact
    20521 taaagactaa atattaatta tttagcgcgg ttagaagtac cgaactcttt aattttacct
    20581 ttaactgttt ctttgatggc ttcacgtgca ggtcctaagt atttacgagg atcgtaaact
    20641 tctttgtcgt tatttaaaac gtcacgaact gcttttgctg aagcgatttg gttttcagtg
    20701 tttacgttaa ttttagctgt accaaatgga attgcttttt ggatatcttt agtcgggata
    20761 ccagtaccac cgtgtaatac taatggtaaa cctgtagata aaccgatttc ttccatttct
    20821 ttaaatccta attttggttc acctttgtat ggaccatgaa ctgaacctaa tgctggcgct
    20881 aatgcatcaa taccagtttt ttcaactagt tcttgacatt ctttaggatc agcataaatg
    20941 atgccgtctg ctacaacatc atcttcttgt ccaccaacag tacctaattc agcttctaca
    21001 gaaacacctt tttcatgagc gtattcaaca acttttttag ttgttgctac gttttcttcg
    21061 aatgggctgt gtgaagcatc gatcattact gatgtgaaac cagcatcgat agcttcttta
    21121 catttttcaa agcttgaacc atggtctaaa tggattgcta caggaatagt gatgtttaag
    21181 tcatgcatta acccttcaac cattttaaca attgtgtaga aaccgctcat gtaacgagca
    21241 gcaccttcag aaacacctaa aattacaggt gcattttctt cttgtgacgc ttctaaaatt
    21301 gcttgagtga attctaggtt attaatattg tattgaccta ccgcataacc attttctttt
    21361 gcatcaatta acatttcttt cattgaaact aaaggcatga aagttcctcc ttgagtgctt
    21421 acgcacgtat ttttcaaagt aattatatca aaaaaatagc gtcaatgcat tgaaatactg
    21481 cagattaaat gtgtattttt tgaaaaatgc gatagttata ctaaatgcaa acgtatacat
    21541 ttttatacaa tttaagccgt tgcataatat gtagtcatta attacaatat tttataagta
    21601 gattggagga taaagatgtc aaaaatctta aacacacaac taataggtat ctttaatagg
    21661 ctagaaaaac aatctttaga aattcaaatg gccgcccaat gtttaattca agcaattggt
    21721 ggcgaaggtt atgtttatgt aaaaggttat gatgatttac agtttttcga aagtttcatt
    21781 ctacatagtg atgagcgttt aaaatctagc cgaaaacttg acgctattaa agactttaaa
    21841 gaaattgact caactgatcg tgtattatta ttcgcaccat tttataatga tcaagttgca
    21901 ttagatattc aaaaacttat cgacttagat atcgatgtgg tcttaataag caacaaacct
    21961 aaaacagatg atttccctga tcatctcgta cattttatcg atttatctac accaagacct
    22021 atagtctaca ctgaagatta cgataagatt gtccaaccac atgcgatggc attgaattat
    22081 gtatactatg acatttatac acaaatgatt gaaatgacaa gagacttaga attataaaag
    22141 tcactattgt tatttcatac cttgtcacta acataatatt aaaattatac gaaaaaccgg
    22201 tactttcaat gtttaacaat gaaagtaccg gttttattag caaatttatt tattttgttg
    22261 atattttaat gaagcttcaa taaatgattt aaaaatcggg tgcggacgat ttggtctaga
    22321 taagaattct gggtggaatt gacaagcaat aaagaaatca tttgtcggaa tctctaccat
    22381 ttctactaaa cgtccatctg gacttgtacc agaaatcacc ataccatttg cttctaattg
    22441 ttctctatag tcattattaa attcataacg atgacgatgt ctttcttcaa tttccgcttt
    22501 accataaaca tcttgtgcca atgtgccttc tttaattgaa catggatata agcctaagcg
    22561 taatgtacca cctaaatctt cgatatcttt ttgttctggt aataaatcta taattgggta
    22621 tggtgttgct gggtctaatt cagctgaatg tgcgccttca aggcctaata cgtttcttga
    22681 aaattcaact gttgcaagtt gcattcctaa acaaatacca aagaatggta cattgttttc
    22741 tctagcatac ttaattgcac taattttacc ttcacttgca cggaaaccaa atccacctgg
    22801 tactaaaata ccgtcgacat ctgcaaggta ttcggctgca ttttcatctg ttacttcact
    22861 tgaatcaatc catctaatgt caatatcttt ggcaaaagga tatccagcat gtttcaatga
    22921 ttcaacaact gataaatatg catcttgtaa gctaacatat ttacctacta aaccaattgt
    22981 aattttacca tctaaattat taacgatatc taacaactgt ttccattcat caagctgtgt
    23041 ttcatatttc gcgtttaatt gtaaacgttt aataacgata tcatccatat tttgttggct
    23101 taattgtaat ggaatttcgt ataaagagtc tgcatcacga cattcaataa cactttcttt
    23161 attaatgtca cagaataatg caattttatc ttttaaatct tgtgtcattt catattcagt
    23221 tcttacaacg attaagtctg gttgaatacc taagcctcgt aattctttaa cactatgttg
    23281 tgttggcttc gttttcattt ctccagcagc tttaatataa ggcagtaatg tacagtgaac
    23341 atacataaca ttttctctac ctaaatcgct acgaatttga cgaatcgctt caataaacgg
    23401 taatgactca atatcacctg ttgtaccgcc aatttcagtg ataacaacgt ctgcattcgt
    23461 actttcccct gcaagtaata aacgttcttt aatttcattt gtaatatgcg gaataacttg
    23521 aactgttccg cctaagtaat caccacgacg ttcttttttc aatacgtgtg aatacacttt
    23581 accggctgtc acatttgaaa acttgtttaa attaatatca ataaatcttt cgtaatgtcc
    23641 taagtctagg tcagtttctg caccatcatc cgttacgaat acttcaccat gttgataagg
    23701 actcattgta cctgggtcaa catttaagta tggatcgaat ttttgaattg ttacatttag
    23761 acctctatct tttaataatc tacctagaga agatgctgta atacccttcc ctaatgatga
    23821 aactacgcca cctgttacaa aaataaattt tgtcatgtct tgacctccta attattaaag
    23881 ggaatgcttc ctcatcaatt gtaaaaatta cgtaaaacat aacattaaag caagctcatt
    23941 caagttgttt atttgtcgaa ttcgacaaaa ttaaattctg tttacttacc gtttccatac
    24001 tgctatgtca ctatgtatat catttacaac tttgattttt gcgttatcat ttcttgattt
    24061 actcaattaa ataaaaaaac gctccctctc acaaattctg taaggggagc gtataaattt
    24121 tatacacgtg tcctatttaa aggagcccga ataaaatttt atcatctttc aactaaaagt
    24181 caaacaaaaa attaatcgtt gaagtcttct tcgtcttcaa acacttcttc gtcttcgtct
    24241 agttcatctt catcttcttc aatgactata tccgaatgat tgatttcttc ttcaacctgc
    24301 tcatcttctg gatcatttag ttcttcttga tcatctgttt gagctggaat atcatcgtcg
    24361 tcatccattt catcttcgcc caataatttt aagttttgat cttcttcatc atctgcatcc
    24421 agaatatcga atttttgaat agttggtgcg attttctctt caatatcatc taccgaatac
    24481 caatcacgta atccccataa attttctcca acatttaaaa aacgaccatc tgtgtttaaa
    24541 tccgtgtaaa attgtacaac acgattttca atttcttcgt actcataatc acctaacgct
    24601 ctaaattcat cgatgatatc atataagttc attgtttcgc ctttatcatt caataatgta
    24661 taagccatat caataaatga tttttcatca accatttgtt ttgtataatc ttgaattttc
    24721 attgcccaat acttcctttc gaaggatata atctaaattt atacgtctga attcatttat
    24781 atatagtaac aaaactaaat tataaatgac aatatcatat ttcgcaatca ctgttagtaa
    24841 tttaaatatt tttcgaaaac gacataatca tcagtttgtt cgatttcaac gaaattacta
    24901 tttaaacata atggtttaaa atcattattc tctccaaaca gaatcatcgt aatcacatta
    24961 aggttgtcat agttttttct taagtaagtt ggcaattgtc gcagagcact tgaaccaata
    25021 ccattaaagc gatatgattt atccacttca atagcagcaa tgttcatgcg ttcaccaatc
    25081 tgctccaatg cgataaagcc aaccttttgg ccatcaatca ttaaattaat gatatgtaca
    25141 ttggtcactt ctgaacgact ttcttcttca agctttaatg atgaaacatg ttttgaatct
    25201 aagtccaagt aatatagtcg ttctttgcca attggtcctt cttctttagt cgctgtatgc
    25261 atgaatccag cacgttcgta taggttccaa gcattgtcat tttccgcatc tactactaga
    25321 tatagatgat taaaatcagg aaatacacct tgaacatatt gcggcaatga catcattatt
    25381 ttcgtgccat atccaaaacc ttgatatttt tcattaatcg ataatgaacg aatataaacg
    25441 acattttcag gtgtatcata accttcatgc tgatagtaac gatgtaatac aaaaaagccc
    25501 accacttcat ttttatcgtt tattgctacg ttagcaatcc tatcagcatc attaattgca
    25561 tcatcaataa cttctttagg taaagatgaa tatatttgtt gtctttcatt caagtcaaaa
    25621 tcattaattg ctgaacgata ttgttcatca aatgctttta aagtgatacc ttcaaaacaa
    25681 agttctttag ccatatattt aacccctatc tttttatata tgaagcatga atccccatat
    25741 tattttacct attatttcac tacgatacca ttatttgctc taagttaaat aaattactta
    25801 tttatatttt ttaaatttca aaattaatgt actattcctc attacttatc attttaatca
    25861 gagacttaca aatacactgc gtacgaacta cttttaatat tgcgtcacta actaacaatc
    25921 ctatacaact tcaaaattcc tagaaaataa tatatataac ttaacgtcca cgtttgtaaa
    25981 acattaattg cgatacgctt acaatgttga aatgcaatgg catcaaattt gttaataatt
    26041 actattatta cttgtttttt agtcatttta ctatactatt atttaaggat agacattaat
    26101 aatgaggtgg gaatcttatg aaagttggca ttgacgctgg cggtacacta attaaaatcg
    26161 ttcaagagca agataaccaa cgtactttta aaactgaatt aactaaaaat attgatcaag
    26221 ttgtggaatg gttaaaccaa cagcaaattg aaaaattatg cttaactgga ggtaatgcag
    26281 gtgtcattgc tgaaaacatc aacattcctg cacaaatttt tgtagagttt gatgctgcat
    26341 ctcaaggact aggtattttg ttaaaagagc aaggccatga cttagcagat tatatttttg
    26401 caaatgttgg taccggtacg tcactacatt atttcgacgg ccaatcacaa cgtcgtgtag
    26461 gcggtatcgg tacaggtgga ggcatgattc aaggactcgg ttatttacta agtcaaatca
    26521 ctgattataa acagttaact gacatggcac aacacggtga tcgtaacact atcgatttaa
    26581 aggttagaca tatttataaa gataccgaac cacccattcc cggtgattta acagcagcta
    26641 attttggtca tgtactacat catttagatg ctgattttac acctagcaat aaattagcag
    26701 ccgttattgg tgtcgttggc gaagttgtta cgacaatggc cattactgtt gcacgtgaat
    26761 ttaaaactga aaatatcgtt tatatcggtt cttcatttca taataacgct ttattacgca
    26821 aagttgtgga agattatact gtgttacgcg gttgcaaacc gtactatgtt gaaaacggcg
    26881 cattttcagg tgctattggt gcattatatt tagaaaaata gaaaaagacc atttatatgc
    26941 gcgttgtttt aaataaacgc tatataaatg gccttttatt ttatggtcta agtgttttga
    27001 atacgtattg ttccatttat tatcacttaa ttacttacaa ctcaattcaa ttaaaatgta
    27061 agttcattag acaatttttg cgctgaaaca attgtaccaa ccacgccagt ttttaaatct
    27121 atcgcaacgg ctttatgatc atctctaagt tcatctagcc attctccgaa aacagaacct
    27181 tcaatcgtaa ttaaatcaca ctcttcaaaa tcttcatctc gaacttttaa cgctctgttt
    27241 ttaatactcc atacaggcat aatatgcggc tttttttcgt cgctatcatt ttcactaatg
    27301 agaataaact gcttactacc ttttttagtt aagccgtata cttcatcata tttcgctaca
    27361 tcttggacaa attctttgat tctaatttta tctaattccg tcatcacttc ttgtgtaaca
    27421 gctacaacgt ttagtagatg cccttcttct tccattgttt gattcataat aatatggtct
    27481 tgttcatcaa acaattcatc taattcataa gtagcaaaac gatcaacgtc catttttaca
    27541 actttatcaa atggaatatc acgacttgtt aaaaatgata cggccacact ttcttctgtc
    27601 caaacgccta catacgcttc acttttataa tttaggcgta ctagtttttt atttttagag
    27661 gcgaaataaa tacattcatt tattaatata tctctaaaga atggatatgc tttataagac
    27721 atgcttgacc ctcacttttt aaaatatgtc atacataaaa tagtaaatga ctccaacaag
    27781 ttagtcaatt agacatatta tgaattcaat ttaatgtcat gtttttcatt ttaaaaattc
    27841 aatagcatta aattttacgt atacacaaat tcggtctata acacgaaaca tatctagtta
    27901 ctaaaaattt caaaacgata acctttagta tacctttatc aacaaaatgg caactcttat
    27961 aacatagtga aataaaagtc gccatttaat attagtatta caaagtattg ccatatcata
    28021 aatttaatta ctgttgacta gaatcgaaaa aagcttgtta caaacgcata ttcagtcaac
    28081 tactggcaac acaacattgt agcgccttat gttccgaact ctaatagtgt tcaagttcag
    28141 ctaaaaatgc gcgttcattc tccagtactg tttcttttga atcaccatgc caaatacagc
    28201 caaaccttga tttaacttta tcttcaccgt accaatcacc atcataataa gataacgggt
    28261 ataaactacc ttctttgaca tatttcaaaa tcgtattgaa gaaatgtgtg ttatcacctt
    28321 ttgaatgata actataaaac ttttgatatc ctgaattcaa ctcgttagca agtaataaca
    28381 tgctcgttga accattttgt ctaaagttta aatcaatcgc ataaacgtta tcatcctcat
    28441 cgacgagtag gtcaaaacca gctacaccaa agaatccttg attcacaccg ttttccataa
    28501 tttgtctacc tgcttctata acgtgctctg gtacattagt agtattttca tttccgttat
    28561 aaaatccgta cttatccgtt aattgcgttg ccgcaccaag atattgaatg cctagcgatt
    28621 ccgagtatgc aaactgaacg caataattcg ctttttcttc aatcttctgt tcgataataa
    28681 gcgaattcgt ttcagcagtt gcctctttaa tacgcgttat cgctttttgt aaatctgcat
    28741 catgataaca aatcataacg ccatagccac ctgctgtcgg aagatcatct cccggcttaa
    28801 ttactaacgg gaattcccaa ttcttaatct cgttttcgaa ttgctcaatt tttacaactt
    28861 ttctttttgg taaaaacttc ccatttgtcc attcaggtat tcttgcttta ttatttaaag
    28921 caacaaataa cgttttatct aatgcataat attgctgatt caagattgtt tcatcatgaa
    28981 tatattgaaa ataaatcttt ttattttcct tatgtgccaa ttgtttgatc aagttttcgt
    29041 aagattgctg attgttaaat gtataaattg agttcggtac ttccttacca ataacttgaa
    29101 atagctgatg caatttgtct gtcgcactag cttcgtgaac aataacaggt aattgatttg
    29161 ctattaataa ctccctacca gttaaaaaat tagattgatg ttcgtccggt ttcaaccatg
    29221 gattcgatat atacgaaggt cttgacgtat agacaacatc tttgtcatat aaatcactta
    29281 acgttaagtt cggctcatta ccattatttg tcattacttc ccattccctt tcaaatgcgc
    29341 atgctcttca ataatgtctt gataaacgtc ttgatttgta attaactcta accccatcaa
    29401 cgccattatt ttagcgcctt taattaatgc ttcatcacca tgtacactcg cagccgcttc
    29461 tctaaatcta tgcgtatgtc ctactaaatt acgtgatcct attttaatat gaggatgtat
    29521 tgttggcaca acatgactta cgttccctgt atccgtagag ccataaccaa aatcatcatc
    29581 aataactgct tcaccaactt cttcagcata tttagcaaat aaatcatcta atttcggcgt
    29641 tttaatgaat tcattcacac cgttttgaat tcgaccaaat tcataatcac aaccagtctg
    29701 tatcgcagct ccacgtgcga tttgatttac tttttctgtt aatatatcca attctttacg
    29761 cgtcattgct ctagtataaa aacgagcatg tgtatagtct ggaataatat tagctgcttt
    29821 cccgccatct aaaatcacac catgcacacg ttgatctttt ttaatatgtt gtcgtagttg
    29881 tgctacacca ttaaaataac taatcatagc gtctaatgca tttaacgctt catctgcatt
    29941 ttcagaggca tgagcacttt ttccgtaaaa tttaacatct aaaacatcca ctgccaaagt
    30001 atcaatcgtt ttataagttt catttcccgg atgaatcatt aaggcaatgt ctatttgatc
    30061 aatcacacca gccttgacat aagaagcttt agcgctacca ttttccccac cttcttcagc
    30121 tggacatcca agaacgacta ctttaccacc aatttggtca atcacttgct tcaaaccaat
    30181 tgcaccaaga acacttgcag ttccaatgat attatgacca caagcatgac ccaatcctgg
    30241 caaagcatcg tattctgcta aaaaacctat agctggcccg tcaagtcctg aatcatatgt
    30301 cgctataaac ccagttgcat gcccagcaat ctcggtttca atttcaaaat catgctcttt
    30361 caaacgatct attaaagttc gagacgcaaa tatttcttca ttaccaagtt caggacgttc
    30421 atgaattcta tgactgattt cgatataact atatttattt gtttctatat aatcgagaat
    30481 ttgttgtttt tcactcattt tttactatct cccttttacc ctacactcat tttaatcatc
    30541 cacaatattt tgttctttca aaatgaatta ttacttattc tatcggtttt atctcatgat
    30601 gtcatctagt ttttctttat ttacaaaatt ttctaataac taaaagcctt tcataaattt
    30661 ataaaacagt ttcaaattgt aaaacataag ccctattgtt acaatttaag tattgttaga
    30721 aacacaactt acaacaaact taagttttat aatgcaacaa atcataagcg ttttatagtt
    30781 ttgagtagtt aataggagga aattcaaaat gacaaaaatg aatgttgaaa gtttcaattt
    30841 agatcatact aaggtggttg ccccatttat tagattagcg ggaacgatgg aaggattaaa
    30901 cggagatgtc attcacaaat acgacattcg tttcaaacaa ccaaacaaag aacatatgga
    30961 tatgcccgga ctacattcat tagaacattt aatggctgaa aatattagaa atcatagtga
    31021 caaagttgtt gatttaagtc ctatgggttg ccaaactggt ttctatgtat catttattaa
    31081 tcatgataat tatgatgatg tattaaatat tgttgaagca actttaaatg atgtgctaaa
    31141 tgctactgaa gtgcctgctt gtaatgaagt acaatgtggc tgggcagcaa gtcattcatt
    31201 agaaggtgct aaaactatcg ctcaagcatt tctagacaaa cgaaacgaat ggcatgatgt
    31261 tttcggtaca ggaaaataaa tcttagtcaa tcaagttaat cagaaaagca gtcgaacaat
    31321 gattttacaa tcgccattgt ccaactgctt tttattatgc ttcaaagtca aaaaatcgaa
    31381 caaatgaaaa agtaaaatct ttaacatttg tccgatttat ttgagaacca ctataatttc
    31441 ttaattagtc ccattaacac gaactgcata ggtaacctta aatatagttg ccatgttggc
    31501 aattgtttat ctcctaaagg taacttttta actgccatat agatattagc tgggaataca
    31561 gctagcaaga atagattgat tgtatttttc aagcattgag atggtctttt aattaaaagt
    31621 gcaagtccaa ataatatctc aaagattcct gtaacaagaa ccgctgtttt tcgaagtggc
    31681 aaacatttcg gtatgatatt tctaaattgt cgttctcgtg taaaatgcaa tacacctatt
    31741 acactaaaac ctattcctaa taaatatctt agtatgttca atcagcttca actcctattc
    31801 tgtaatgatt ttatgaatta atgtaggcga tacaacatga tcagcaattg ttatgcttga
    31861 atctaatttt ttaacaacat cgtctacatc ttggcgatta ctgtgaatcg ttaataatga
    31921 ttctccttct tctactttat caccaatttt tttatttaaa acaataccaa ccgctaaatc
    31981 aatatcatcc tcttttgtta aacgtcccgc tcctaacatc atcgaagcga cacctatatc
    32041 gttagagact aattcagtca cataacctga ttttttagct ttatattcaa tttgatattg
    32101 agcttgtggc aaacgctctg gatggtcaat aacagtttcg tcgccacctt ggtttttaat
    32161 aaatgttttg aatttttcta atgctgcacc tgaattaatt gcctcaatta gcaacgctct
    32221 cgcttcttca agcgtttcag ctttgtttgc aagtacaacc atttgagaac ctaatgttaa
    32281 tacaagttct gttaaatctt tcggaccttg tcctttcaac gtatcaattg cttcttgtaa
    32341 ctcaagcgca ttgccaatcg cacgtccaag tggctgattc atatcagaaa taatcgccat
    32401 cgtattacgt cccacattat taccaatacg taccattgcg tgcgctaatg cttcagcatc
    32461 ttctaatgtt ttcataaatg caccgctacc agtttttaca tctaatacaa ttgcatctgc
    32521 accagcagca atctttttac tcataattga agaggcaatt aatggtattg aattgacagt
    32581 accagtaaca tcccttaagg catataattt tttgtctgca ggagttaaat ttcctgattg
    32641 tcctacaact gccactttat tttcattaac caatttcaca aatgttgctt catctatttc
    32701 aacatgaaaa ccatcaattg cttctaattt atcaatcgta ccacctgtat gacctaatcc
    32761 acgcccactc atttttgcaa caggaacatc tacagctgct actaatggtg ctaaaaccaa
    32821 tgtagttgta tctcctacac cacctgttga gtgcttatct actttgacac ctttaatatc
    32881 actcaaatct atcatatcac cagaattaac catagccatc gttaatgctg cacgctcatc
    32941 atcattcata tcttggaaat aaatcgccat tgctaaactt gatgcttggt aatcaggaat
    33001 atccccttta acatagccgc caataaagaa attaatttct tccgttgtta gtgtatgacc
    33061 gtcacgcttt ttctcaataa tgtctatcat tctcattttt atcatccttt tcttaaaaag
    33121 cttaggacaa agcatctgcg ctttctctag tccattttta aaagcacaag cgaaaattat
    33181 tatagcaagc tatcgatttc atttttaata tcacaatttc aatgcgatgt tattattctt
    33241 aaatagattg gttataacgt taaagtccct attaaattat cttagaatca tcatggcatt
    33301 tatgatgtct taaagctgat atcgacatac ttatatatgg ttacgatgtc ccatgcttac
    33361 atatttttat aaaattagta atctgaatct gcttctaaac cttgcataat ttgaac
//
LOCUS       DALAGD010000020        33203 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.020, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000020 DALAGD010000000
VERSION     DALAGD010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 33203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33203
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.020"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            108..1394
                     /gene="rseP"
                     /locus_tag="O2701_000737"
     CDS             108..1394
                     /gene="rseP"
                     /locus_tag="O2701_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HCZ4292643.1"
                     /translation="MSYLVTIIAFIIVFGVLVTVHEYGHMFFAKRAGIMCPEFAIGMG
                     PKIFSFRKNETLYTIRLLPVGGYVRMAGDGLEEPPVEPGMNVKIKLNEENEITHIILD
                     DHHKFQQIEAIEVKKCDFKDDLFIEGITAYDNERHHFKIARKSFFVENGSLVQIAPRD
                     RQFAHKKPWPKFLTLFAGPLFNFILALVLFIGLAYYQGTPTSTVEQVADKYPAQQAGL
                     QKGDKIVQIGKYKISEFDDVDKALDKVKDNKTTVKFERDGKTKSVELTPKKTEKKLTK
                     VSSETKYVLGFQPASEHTLFKPIVFGFKSFLIGSTYIFTAVVGMLASIFTGGFSFDML
                     NGPVGIYHNVDSVVKAGIISLIGYTALLSVNLGIMNLIPIPALDGGRILFVIYEAIFR
                     KPVNKKAETTIIAIGAIFMVVIMILVTWNDIRRYFL"
     gene            1414..3117
                     /locus_tag="O2701_000738"
     CDS             1414..3117
                     /locus_tag="O2701_000738"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCZ4292644.1"
                     /translation="MKQSKVFIPTMRDVPSEAEAQSHRLLLKSGLIKQSTSGIYSYLP
                     LATRVLNNITAIVRQEMERIDSVEILMPALQQAELWEESGRWGAYGPELMRLQDRHGR
                     QFALGPTHEELVTSIVRNELKSYKQLPMTLFQIQSKFRDEKRPRFGLLRGREFIMKDA
                     YSFHADEASLDQTYQDMYQAYSRIFERVGINARPVVADSGAIGGSHTHEFMALSAIGE
                     DTIVYSKESDYAANIEKAEVVYEPNHKHTTVQPLEKIETPNVKTAQELADFLGRPVDE
                     IVKTMIFKVDGEYIMVLVRGHHEINDIKLKSYFGTDNIELATQDEIVNLVGANPGSLG
                     PVIDKEIKIYADNFVQDLNNLVVGANEDGYHLINVNVGRDFNVDEYGDFRFILEGEKL
                     SDGSGVAHFAEGIEVGQVFKLGTKYSESMNATFLDNQGKAQSLIMGCYGIGISRTLSA
                     IVEQNHDDNGIVWPKSVTPFDLHLISINPKKDDQRELADALYAEFNTKFDVLYDDRQE
                     RAGVKFNDADLIGLPLRIVVGKRASEGIVEVKERLTGDSEEVHIDDLMTVITNKYDNL
                     K"
     gene            3381..7691
                     /locus_tag="O2701_000739"
     CDS             3381..7691
                     /locus_tag="O2701_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCZ4292645.1"
                     /translation="MTEQQKFKVLADQIKISNQLDAEILNSGELTRIDVSNKNRTWEF
                     HITLPQFLAHEDYLLFINAIEQEFKDIANVTCRFTVTNGTNQDEHAIKYFGHCIDQTA
                     LSPKVKGQLKQKKLIMSGKVLKVMVSNDIERNHFDKACNGSLIKAFRNCGFDIDKIIF
                     ETNDNDQEQNLASLEAHIQEEDEQSARLATEKLEKMKAEKAKQQDNNESAVDKCQIGK
                     PIQIENIKPIESIIEEEFKVAIEGVIFDINLKELKSGRHIVEIKVTDYTDSLVLKMFT
                     RKNKDDLEHFKALSVGKWVRAQGRIEEDTFIRDLVMMMSDIEEIKKATKKDKAEEKRV
                     EFHLHTAMSQMDGIPNIGAYVKQAADWGHPAIAVTDHNVVQAFPDAHAAAEKHGIKMI
                     YGMEGMLVDDGVPIAYKPQDVVLKDATYVVFDVETTGLSNQYDKIIELAAVKVHNGEI
                     IDKFERFSNPHERLSETIINLTHITDDMLVDAPEIEEVLTEFKEWVGDAIFVAHNASF
                     DMGFIDTGYERLGFGPSTNGVIDTLELSRTINTEYGKHGLNFLAKKYGVELTQHHRAI
                     YDTEATAYIFIKMVQQMKELGVLNHNEINKKLSNEDAYKRARPSHVTLIVQNQQGLKN
                     LFKIVSASLVKYFYRTPRIPRSLLDEYREGLLVGTACDEGELFTAVMQKDQSQVEKIA
                     KYYDFIEIQPPALYQDLIDRELIRDTETLHEIYQRLIHAGDTAGIPVIATGNAHYLFE
                     HDGIARKILIASQPGNPLNRSTLPEAHFRTTDEMLNEFHFLGEEKAHEIVVKNTNELA
                     DRIERVVPIKDELYTPRMEGANEEIRELSYANARKLYGEDLPQIVIDRLEKELKSIIG
                     NGFAVIYLISQRLVKKSLDDGYLVGSRGSVGSSFVATMTEITEVNPLPPHYICPNCKT
                     SEFFNDGSVGSGFDLPDKTCETCGAPLIKEGQDIPFETFLGFKGDKVPDIDLNFSGEY
                     QPNAHNYTKVLFGEDKVFRAGTIGTVAEKTAFGYVKGYLNDQGIHKRGAEIDRLVKGC
                     TGVKRTTGQHPGGIIVVPDYMDIYDFTPIQYPADDQNSAWMTTHFDFHSIHDNVLKLD
                     ILGHDDPTMIRMLQDLSGIDPKTIPVDDKEVMQIFSTPESLGVTEDEILCKTGTFGVP
                     EFGTGFVRQMLEDTKPTTFSELVQISGLSHGTDVWLGNAQELIKTGICDLSSVIGCRD
                     DIMVYLMYAGLEPSMAFKIMESVRKGKGLTEEMIETMKENEVPDWYLDSCLKIKYMFP
                     KAHAAAYVLMAVRIAYFKVHHPLYYYASYFTIRASDFDLITMIKDKTSIRNTVKDMYS
                     RYMDLGKKEKDVLTVLEIMNEMAHRGYRMQPISLEKSQAFEFIIEGDTLIPPFISVPG
                     LGENVAKRIVEARDDGPFLSKEDLNKKAGLSQKIIEYLDELGSLPNLPDKAQLSIFDM
                     "
     gene            7981..8448
                     /gene="rimP"
                     /locus_tag="O2701_000740"
     CDS             7981..8448
                     /gene="rimP"
                     /locus_tag="O2701_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCZ4292646.1"
                     /translation="MSKITEQVEVIVKPIMEDLNFELVDVEYVKEGRDHFLRISIDKE
                     GGVDLNDCTLASEKISEAMDANDPIPEMYYLDVASPGAERPIKKEQDFQNAITKPVFV
                     SLYVPIEGEKEWLGILQEVNNETIVVQVKIKARTKDIEIPRDKIAKARHAVMI"
     gene            8469..9644
                     /gene="nusA"
                     /locus_tag="O2701_000741"
     CDS             8469..9644
                     /gene="nusA"
                     /locus_tag="O2701_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCZ4292647.1"
                     /translation="MSSNELLLATEYLEKEKKIPRAVLIDAIEAALITAYKKNYDSAR
                     NVRVELNMDQGTFKVIARKDVVEEVFDDRDEVDLSTALVKNPAYEIGDIYEEDVTPKD
                     FGRVGAQAAKQAVMQRLRDAEREILFEEFIDKEEDILTGIIDRVDHRYVYVNLGRIEA
                     VLSEAERSPNEKYIPNERIKVYVNKVEQTTKGPQIYVSRSHPGLLKRLFEQEVPEIYD
                     GTVIVKSVAREAGDRSKISVFSENNDIDAVGACVGAKGARVEAVVEELGGEKIDIVQW
                     NEDPKVFVKNALSPSQVLEVIVDETNQSTVVVVPDYQLSLAIGKRGQNARLAAKLTGW
                     KIDIKSETDAREAGIYPVVEAEKVTEEDVALEDADTTESTEEVNDVSVETNVEKESE"
     gene            9665..9949
                     /locus_tag="O2701_000742"
     CDS             9665..9949
                     /locus_tag="O2701_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HCZ4292648.1"
                     /translation="MKKKKIPMRKCILSNEMHPKKDMIRVVVNKEGEIFADVTGKKQG
                     RGAYVSKDVAMVEKAQQKEILEKYFKASKEQLDPVYKEIIRLIYREEIPK"
     gene            9946..10263
                     /locus_tag="O2701_000743"
     CDS             9946..10263
                     /locus_tag="O2701_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ family RNA-binding protein"
                     /protein_id="HCZ4292649.1"
                     /translation="MSIDQILNFLGLAMRAGKVKTGESVIVNEIKKGNLKLVIVANDA
                     SDNTAKLITDKCKSYKVPFRKFGNRNELGIALGKGERVNVGITDPGFAKKLLSMIDEY
                     HKE"
     gene            10268..12385
                     /gene="infB"
                     /locus_tag="O2701_000744"
     CDS             10268..12385
                     /gene="infB"
                     /locus_tag="O2701_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCZ4292650.1"
                     /translation="MSKQRIYEYAKELNLKSKEIIDELKSMNIEVSNHMQALEDDQIK
                     ALDKKFKKEQKNDNKQSTQNNHQKSNNQNQNKGQQKDNKKNQQQNNKGNKGNKKNNRN
                     NKKNNKNNKPQNQPAAPKEIPSKVTYQEGITVGEFADKLNVESSEIIKKLFLLGIVAN
                     INQSLNQETIELIADDYGVEVEEEVVINEEDLSIYFEDEKDDPEAIERPAVVTIMGHV
                     DHGKTTLLDSIRHTKVTAGEAGGITQHIGAYQIENDGKKITFLDTPGHAAFTTMRARG
                     AQVTDITILVVAADDGVMPQTIEAINHAKEAEVPIIVAVNKIDKPTSNPDRVMQELTE
                     YGLIPEDWGGETIFVPLSALSGDGIDDLLEMIGLVAEVQELKANPKNRAVGTVIEAEL
                     DKSRGPSASLLVQNGTLNVGDAIVVGNTYGRIRAMVNDLGQRIKTAGPSTPVEITGIN
                     DVPQAGDRFVVFSDEKQARRIGESRHEASIIQQRQESKNVSLDNLFEQMKQGEMKDLN
                     VIIKGDVQGSVEALAASLMKIDVEGVNVRIIHTAVGAINESDVTLANASNGIIIGFNV
                     RPDSGAKRAAEAENVDMRLHRVIYNVIEEIESAMKGLLDPEFEEQVIGQAEVRQTFKV
                     SKVGTIAGCYVTEGKITRNAGVRIIRDGIVQYEGELDTLKRFKDDAKEVAKGYECGIT
                     IENYNDLKEGDVIEAFEMVEIKR"
     gene            12771..13121
                     /gene="rbfA"
                     /locus_tag="O2701_000745"
     CDS             12771..13121
                     /gene="rbfA"
                     /locus_tag="O2701_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCZ4292651.1"
                     /translation="MSSMRAERVGEQMKKELMDIINNKVKDPRVGFITITDVVLTNDL
                     SQAKVFLTVLGNDKEVENTFKALDKAKGFIKSELGSRMRLRIMPELMYEYDQSIEYGN
                     KIERMIQDLHKQDR"
     gene            13290..14207
                     /gene="truB"
                     /locus_tag="O2701_000746"
     CDS             13290..14207
                     /gene="truB"
                     /locus_tag="O2701_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCZ4292652.1"
                     /translation="MYNGILPVYKERGLTSHDVVFKLRKILKTKKIGHTGTLDPEVAG
                     VLPVCIGNATRVSDYVMDMGKAYEATVSIGRSTTTEDQTGDTLETKGVHSADFNKDDI
                     DRLLESFKGIIEQIPPMYSSVKVNGKKLYEYARNNETVERPKRKVNIKDIGRISELDF
                     KENECHFKIRVICGKGTYIRTLATDIGVKLGFPAHMSKLTRIESGGFVLKDSLTLEQI
                     KELHEQDSLQNKLFPLEYGLKGLPSIKIKDSHIKKRILNGQKFNKNEFDNKIKDQIVF
                     IDDDSEKVLAIYMVHPTKESEIKPKKVFN"
     gene            14222..15193
                     /gene="ribF"
                     /locus_tag="O2701_000747"
     CDS             14222..15193
                     /gene="ribF"
                     /locus_tag="O2701_000747"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HCZ4292653.1"
                     /translation="MKVIEVTHPIQSKQYITEDVAMAFGFFDGMHKGHDKVFDILNEI
                     AEARSLKKAVMTFDPHPSVVLNPKRKRTTYLTPLSDKIEKISQHDIDYCIVVNFSSRF
                     ANVSVEDFVENYIIKNNVKEVIAGFDFTFGKFGKGNMTVLQEYDAFNTTIVSKQEIEN
                     EKISTTSIRQDLINGELQKANDALGYIYSIKGTVVQGEKRGRTIGFPTANIQPSDDYL
                     LPRKGVYAVSIEIGTENKLYRGVANIGVKPTFHDPNKAEVVIEVNIFDFEDNIYGERV
                     TVNWHHFLRPEIKFDGIDPLVKQMNDDKSRAKYLLAVDFGDEVAYNI"
     gene            15308..15577
                     /gene="rpsO"
                     /locus_tag="O2701_000748"
     CDS             15308..15577
                     /gene="rpsO"
                     /locus_tag="O2701_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCZ4292654.1"
                     /translation="MAISQERKNEIIKEYRVHETDTGSPEVQIAVLTAEINAVNEHLR
                     THKKDHHSRRGLLKMVGRRRHLLNYLRSKDIQRYRELIKSLGIRR"
     gene            15947..18043
                     /gene="pnp"
                     /locus_tag="O2701_000749"
     CDS             15947..18043
                     /gene="pnp"
                     /locus_tag="O2701_000749"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCZ4292655.1"
                     /translation="MSQEKKVFKTEWAGRSLTIETGQLAKQANGAVLVRYGDTVVLST
                     ATASKEPRDGDFFPLTVNYEEKMYAAGKIPGGFKKREGRPGDDATLTARLIDRPIRPL
                     FPKGYKHDVQIMNMVLSADPDCSPQMAAMIGSSMALSVSDIPFQGPIAGVNVGYIDGK
                     YIINPTVEEKEVSRLDLEVAGHKDAVNMVEAGASEITEQEMLEAIFFGHEEIQRLVDF
                     QQQIVDHIQPVKQEFIPAERDEALVERVKSLTEEKGLKETVLTFDKQQRDENLDNLKE
                     EIVNEFIDEEDPENELLIKEVYAILNELVKEEVRRLIADEKIRPDGRKPDEIRPLDSE
                     VGILPRTHGSGLFTRGQTQALSVLTLGALGDYQLIDGLGPEEEKRFMHHYNFPNFSVG
                     ETGPVRAPGRREIGHGALGERALKYIIPDTADFPYTIRIVSEVLESNGSSSQASICGS
                     TLALMDAGVPIKAPVAGIAMGLVTREDSYTILTDIQGMEDALGDMDFKVAGTKEGITA
                     IQMDIKIDGLTREIIEEALEQARRGRLEIMNHMLQTIDQPRTELSAYAPKVVTMTIKP
                     DKIRDVIGPGGKKINEIIDETGVKLDIEQDGTIFIGAVDQAMINRAREIIEEITREAE
                     VGQTYQATVKRIEKYGAFVGLFPGKDALLHISQISKNRIEKVEDVLKIGDTIEVKITE
                     IDKQGRVNASHRALEE"
     gene            18279..19952
                     /locus_tag="O2701_000750"
     CDS             18279..19952
                     /locus_tag="O2701_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCZ4292656.1"
                     /translation="MSLIKKKNKDIRIIPLGGVGEIAKNMYIVEVDDEMFMLDAGLMF
                     PEDEMLGIDIVIPDISYVLENKDKLKGIFLTHGHEHAIGAVSYVLEQLDAPVYGSKLT
                     IALIKENMKARNIDKKVRYYTVNNDSIMRFKNVNISFFNTTHSIPDSLGVCIHTSYGA
                     IVYTGEFKFDQSLHGHYAPDIKRMAEIGEEGVFVLISDSTEAEKPGYNTPENVIEHHM
                     YDAFAKVRGRLIVSCYASNFIRIQQVLNIASKLNRKVSFLGRSLESSFNIARKMGYFD
                     IPKDLLIPITEVDNYPKNEVIIIATGMQGEPVEALSQMAQHKHKIMNIEEGDSVFLAI
                     TASANMEVIIANTLNELVRAGAHIIPNNKKIHASSHGCMEELKMMINIMKPEYFIPVQ
                     GEFKMQIAHAKLAAEAGVAPEKIFLVEKGDVINYNGKDMILNEKVNSGNILIDGIGIG
                     DVGNIVLRDRHLLAEDGIFIAVVTLDPKNRRIAAGPEIQSRGFVYVRESEDLLREAEE
                     KVREIVEAGLQEKRIEWSEIKQNMRDQISKLLFESTKRRPMIIPVISEI"
     gene            20208..22577
                     /locus_tag="O2701_000751"
     CDS             20208..22577
                     /locus_tag="O2701_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCZ4292657.1"
                     /translation="MAQAKKKSTAKKKTTSKKRTNSRKKKNDNPIRYVIAILVVVLMV
                     LGVFQLGIIGRLIDSFFNYLFGYSRYLTYILVLLATGFITYSKRIPKTRRTAGSIVLQ
                     IALLFVSQLVFHFNSGIKAEREPVLSYVYQSYQHSHFPNFGGGVLGFYLLELSVPLIS
                     LFGVCIITILLLCSSVILLTNHQHREVAKVALENIKAWFGSFNEKMSERNQEKQLKRE
                     EKARLKEEQKARQNEQPQIKDVSDFTEVPQERDIPIYGHTENESKSQSQPSRKKRVFD
                     AENSSNNIVNHHQADQQEQLTEQTHNSVESENTIEEAGEVTNVSYVVPPLTLLNQPAK
                     QKATSKAEVQRKGQVLENTLKDFGVNAKVTQIKIGPAVTQYEIQPAQGVKVSKIVNLH
                     NDIALALAAKDVRIEAPIPGRSAVGIEVPNEKISLVSLKEVLDEKFPSNNKLEVGLGR
                     DISGDPITVPLNEMPHLLVAGSTGSGKSVCINGIITSILLNAKPHEVKLMLIDPKMVE
                     LNVYNGIPHLLIPVVTNPHKAAQALEKIVAEMERRYDLFQHSSTRNIKGYNELIRKQN
                     QELDEKQPELPYIVVIVDELADLMMVAGKEVENAIQRITQMARAAGIHLIVATQRPSV
                     DVITGIIKNNIPSRIAFAVSSQTDSRTIIGTGGAEKLLGKGDMLYVGNGDSSQTRIQG
                     AFLSDQEVQDVVNYVVEQQQANYVKEMEPDAPVDKSEMKSEDALYDEAYLFVVEQQKA
                     STSLLQRQFRIGYNRASRLMDDLERNQVIGPQKGSKPRQVLIDLNNDEV"
     gene            22582..23295
                     /locus_tag="O2701_000752"
     CDS             22582..23295
                     /locus_tag="O2701_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCZ4292658.1"
                     /translation="MSEMNAVYNVKQYILNLIKQNKLEYGDQLPSNLSIARELNVKTD
                     DVYEAIQALITEQVIKDNFEEGTSVKSLPPFFYPLNELISIGQMIKNAGFECGTEYLN
                     FDEQPATMLDANLLSVEEGYPVTIIERLRTADGEPVVYCLDKIAKKELTCTEYQMSNG
                     SILSAIKEQSNHNICYADTEIEAVNYEPRISEVLNASPHEGLILLKITHYNESDEPIL
                     YSLNYMKNSLVQFKITRKI"
     gene            23326..24591
                     /locus_tag="O2701_000753"
     CDS             23326..24591
                     /locus_tag="O2701_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCZ4292659.1"
                     /translation="MSRQSQPNIHIKVSPTTKFKTTTIVFKFMAPLEYDTITARSLLS
                     KLLVRATKKWPTDKSFNNHLADLYGAYVNSTISKFKDQHVITFSLEIVNERYLRNGES
                     LFNQGLDLLQEIIWNPLIENKAFNDNFVNQEKTLLAKKIEAMVDNKAQYSFLKLLDHM
                     FENEAYKYLSTGQLEQIPHITAETLYHTYQSMINNDQCSVYVVGNVEPESVEKQIREK
                     FALKPFDKHQFQHSTHHLHDEEVDYIVEYDDVDQAKLNMGYRFPTQYGQSGYAAFVVF
                     NMMFGGDPSSVLFNEVREKQSLAYSIHSQIDGKNGYLFVLSGVSSDKYETAKDTIISE
                     FEKIKAGDFTEEKLELAKKVIISHRYESEDRPKSIIEIMHNQILLEQPQSKETFINDI
                     QKVSREDIVSVAEKAFLDTIYVLTKGGDK"
     gene            24591..25877
                     /locus_tag="O2701_000754"
     CDS             24591..25877
                     /locus_tag="O2701_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCZ4292660.1"
                     /translation="MKERYYELIDERVFEQELENGLRLFIIPKPGFQKTFVTYTTQFG
                     SLDNQFKPLGQDQFVTVPDGVAHFLEHKLFEKEEEDLFTAFAEDNAQANAFTSFDRTS
                     YLFSATDNIENNIKRLLTMVETPYFTKETVDKEKGIIAEEIKMYQEQPGYKLMFDTLR
                     AMYQQHPIRVDIAGSVESIYDITKDDLYLCYETFYHPSNMVLFVVGDVDPEAICRIVK
                     QHEDARNKVNQPKIERGLVDEPEDVKEAFVTESMKIQSPRLMLGFKNKPLQEAPQKYV
                     QRDLEMSLFFELIFGEETDFYQNLLNEGLIDDTFGYQFVLEPTYSFSIVTSATEEPDK
                     LKKLLLDELRDKKGNFQDAEAFELLKKQFIGEFISSLNSPEYIANQYTKLYFEGVSVF
                     DMLDIVENITLDSINETSSLYLNLDQQVDSRLEIKK"
     gene            25877..26581
                     /locus_tag="O2701_000755"
     CDS             25877..26581
                     /locus_tag="O2701_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCZ4292661.1"
                     /translation="MKALVLGGSGSIGSEIVKQLLTDGFEVYVQYYRTDINELTSKFN
                     DDKVRFIQADLSQTIDIDKTFGDIKSLDCLIYASGQSLYGVLQDMKDHDIDACYQLNV
                     LQLIRLCRYFVDVLRQSANGRIIVISSIWGETGASMETIYSAMKSAQLGFVKALSQEL
                     ALTSVTVNAIAPGFVAGNMASEWQEDELQAMITELPQQRLVLPSEVAHTCAYLYHPNA
                     RSVTGTIQKVNGAWYI"
     gene            26686..27513
                     /locus_tag="O2701_000756"
     CDS             26686..27513
                     /locus_tag="O2701_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfK family protein"
                     /protein_id="HCZ4292662.1"
                     /translation="MTVAEKKEWYLEYEIEINRPGLLGDVSSLLGMLGISIVTINGVD
                     QGKRGLLIKTDNLEKVERFEQIARGINEIEITKLKKPELRDRLAVRHGRYIEQDAKDK
                     KTFRFEREDLGLLVDFLAELFKEEGHKLIGIRGMPRVGKTESIVAGSVCAHKRWLFIS
                     STLIKQTVRSSLIKGEYDANHVYIIDGAVTARESNPKHQELVNEVMTLPSIKVVEHPD
                     LFVETSTCTMEDFDYIIELRENENQEIHYEEMKKQTVQSKNNLDFGDPFGGGFGFFE"
     gene            27532..27924
                     /locus_tag="O2701_000757"
     CDS             27532..27924
                     /locus_tag="O2701_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCZ4292663.1"
                     /translation="MKTVGEALKGRRERLGMILTELEQRTGIKREMLVHIENNEFDQL
                     PNKNYSEGFIRKYASVVNIEPNQLIQAHQDEIPSNQAEWDEVITVFNNNKDLDYKSKS
                     KEPIQLLVIMGITVLITLLLWIMLVLIF"
     gene            27958..28536
                     /gene="pgsA"
                     /locus_tag="O2701_000758"
     CDS             27958..28536
                     /gene="pgsA"
                     /locus_tag="O2701_000758"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCZ4292664.1"
                     /translation="MNIPNQITVFRVVLIPVFILFALVDFGFGNVSFLGGYEIRIELL
                     ISGFIFILASLSDFVDGYLARKWNLVTNMGKFLDPLADKLLVASALIVLVQLGLTNSV
                     VAIIIIAREFAVTGLRLLQIEQGFVSAAGQLGKIKTAVTMVAITWLLLGDPLATLIGL
                     SLGQILLYIGVIFTILSGIEYFYKGRDVFKQK"
     gene            28763..29914
                     /locus_tag="O2701_000759"
     CDS             28763..29914
                     /locus_tag="O2701_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HCZ4292665.1"
                     /translation="MSIAIIAVGSELLLGQIANTNGQFLSKVFNEIGQNVLEHKVIGD
                     NKKRLESSVRHALEKYDTVILTGGLGPTKDDLTKHTVAQIVGKDLVIDEPSLKYIESY
                     FEEQGQEMTPNNKQQALVIEGSTVLTNHHGMAPGMMVNFENKQIILLPGPPKEMQPMV
                     KNELLSHFINHNRIIHSELLRFAGIGESKVETILIDLIDKQTNPTIAPLAGSHEVYIR
                     LTANADSKEQAQSLIQPVKQEILDRIGEYYYGSDDTLIEQAVIKKIHEPFVIYDGITN
                     GALYHRLKEVDLNDVLKGMINHNENFVDINKPIEQQLKDAVQFVNKLFNVSSAIILLE
                     YDGVVHIGYDNNFEFKTEQFKMSKSRNLLKNRSQNYALIRLLNWLRTTN"
     gene            30079..31122
                     /gene="recA"
                     /locus_tag="O2701_000760"
     CDS             30079..31122
                     /gene="recA"
                     /locus_tag="O2701_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCZ4292666.1"
                     /translation="MDNDRQKALDTVIKNMEKSFGKGAVMKLGDNIGRRVSTTSTGSV
                     TLDNALGVGGYPKGRIIEIYGPESSGKTTVALHAIAEVQSNGGVAAFIDAEHALDPEY
                     AQALGVDIDNLYLSQPDHGEQGLEIAEAFVRSGAVDIVVVDSVAALTPKAEIEGEMGD
                     THVGLQARLMSQALRKLSGAISKSNTTAIFINQIREKVGVMFGNPETTPGGRALKFYS
                     SVRLEVRRAEQLKQGQEIVGNRTKIKVVKNKVAPPFRVAEVDIMYGQGISKEGELIDL
                     GVENDIVDKSGAWYSYNGERMGQGKENVKMYLKENPQIKEEIDRKLREKLGISDGDVE
                     ETEDAPKSLFDEE"
     gene            31476..33035
                     /gene="rny"
                     /locus_tag="O2701_000761"
     CDS             31476..33035
                     /gene="rny"
                     /locus_tag="O2701_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HCZ4292667.1"
                     /translation="MNLLSLLLILLGIILGVVGGYVVARNLLLQKQSQARQTAEDIVN
                     QAHKEADNIKKEKLLEAKEENQILREQTEAELRERRSELQRQETRLLQKEENLERKSD
                     LLDKKDEILEQKESKIEEKQQQVDAKESSVQTLIMKHEQELERISGLTQEEAINEQLQ
                     RVEEELSQDIAVLVKEKEKEAKEKVDKTAKELLATAVQRLAADHTSESTVSVVNLPND
                     EMKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARTALVNLVSDG
                     RIHPGRIEDMVEKARKEVDDIIREAGEQATFEVNAHNMHPDLVKIVGRLNYRTSYGQN
                     VLKHSIEVAHLASMLAAELGEDETLAKRAGLLHDVGKAIDHEVEGSHVEIGVELAKKY
                     GENETVINAIHSHHGDVEPTSIISILVAAADALSAARPGARKETLENYIRRLERLETL
                     SESYDGVEKAFAIQAGREIRVIVSPEEIDDLKSYRLARDIKNQIEDELQYPGHIKVTV
                     VRETRAVEYAK"
BASE COUNT        12154 a         4482 c         6490 g        10077 t
ORIGIN      
        1 acgttatatt ttgtgatttg tcagtatcat attataatga cttatgttac gtatacagca
       61 atcattttta aaataaaaga aatttataaa caatcgaggt gtagcgagtg agctatttag
      121 ttacaataat tgcatttatt attgtttttg gtgtactagt aactgttcat gaatatggcc
      181 atatgttttt tgcgaaaaga gcaggcatta tgtgtccaga atttgcgatc ggtatggggc
      241 caaaaatttt tagttttaga aaaaatgaaa cactttacac tattaggtta ttgcctgttg
      301 gtggatatgt tcgtatggca ggagatggct tagaagagcc accagtcgag cccggtatga
      361 acgttaaaat taaacttaat gaagaaaatg aaataacaca tatcatatta gatgatcatc
      421 ataagtttca acaaattgaa gcgatcgaag ttaaaaaatg tgattttaag gatgacttat
      481 tcatagaagg tatcactgct tatgataatg aaagacatca ttttaaaatt gctagaaagt
      541 ctttctttgt tgaaaatggt agcttagttc aaattgctcc gagagacaga caatttgcac
      601 ataaaaagcc atggccgaaa tttttaacat tatttgcggg accgttattt aactttatat
      661 tagctttagt cctatttatt ggtcttgcat attatcaagg cacgcctacg tctactgtag
      721 aacaagtcgc agataagtat ccagctcaac aagcaggatt acaaaaaggt gataagatcg
      781 tccaaattgg caaatataaa atatctgaat ttgatgatgt tgataaggcg ttagataaag
      841 ttaaagataa taagacgact gttaaatttg aacgtgatgg taaaacaaag tcagttgaat
      901 taacacctaa aaagactgaa aaaaaactga ctaaagtaag ttcagagacg aagtatgttc
      961 tcggattcca accagcgagt gaacatacac tttttaaacc aattgtattc ggatttaaaa
     1021 gctttttaat cggtagtact tatattttta cagctgtagt aggtatgttg gctagtatat
     1081 ttacgggcgg attctcattt gatatgttaa atggtccggt tggtatttat cataacgtcg
     1141 actcagttgt taaagcgggt atcattagct taattggtta cactgcgtta ttaagtgtaa
     1201 acttaggtat tatgaattta attcctattc ctgcactaga cggtggtcgt attttatttg
     1261 ttatatatga agcgattttc agaaaaccag ttaataaaaa agcggaaaca acgattattg
     1321 ctattggtgc cattttcatg gtcgttataa tgatattagt aacgtggaat gatattcgac
     1381 gatatttctt ataatttagg aggataaata attatgaagc aatccaaagt ttttatacca
     1441 acgatgcgtg acgtgccatc agaagcagaa gcacaaagtc atcgtttatt attgaaatcg
     1501 ggtttgataa aacaaagtac aagtgggatt tatagttatt taccgctagc aacacgtgtg
     1561 ttaaataata ttactgcaat tgtgcgacaa gaaatggaac gtatcgattc tgttgaaatt
     1621 ttaatgccag cgttacaaca agctgaatta tgggaagaat caggacgttg gggtgcatat
     1681 ggcccagaat taatgcgttt acaagataga catggaagac aatttgcatt aggtccaaca
     1741 catgaagaat tagttacatc aatagtaaga aatgaattga aatcatacaa acaattaccg
     1801 atgacattat tccaaattca atctaaattc cgtgatgaaa agagaccacg ttttggttta
     1861 cttcgtgggc gtgaatttat tatgaaagat gcatattcat tccatgctga cgaggcatca
     1921 ttagatcaaa cgtatcaaga tatgtatcaa gcgtatagcc gtatttttga gagagttggc
     1981 attaacgcaa gaccagtagt tgcagattca ggtgctatag gcggtagcca tacacatgaa
     2041 tttatggcat taagtgctat cggtgaggat acaatcgttt acagtaaaga aagtgattat
     2101 gctgctaaca tcgaaaaagc agaagtcgtt tacgaaccaa atcataagca tactactgtg
     2161 caacctttag aaaaaattga aacaccaaat gttaagactg cgcaagaatt ggcagacttc
     2221 ttaggtagac cagtagatga aatcgttaaa acgatgattt tcaaagttga tggcgaatat
     2281 attatggttt tagtgcgtgg ccatcatgaa attaatgaca ttaaattaaa atcttatttc
     2341 ggcacagata atattgaatt agcaacacaa gacgaaattg ttaatttagt tggtgcaaat
     2401 cctggttcac taggtcctgt aattgataaa gaaatcaaaa tttatgcaga taattttgtg
     2461 caagatttaa ataatttagt tgtcggtgct aacgaagatg gttatcactt aattaatgta
     2521 aatgtaggta gagacttcaa cgttgatgaa tatggcgatt tccgttttat tttagaaggc
     2581 gaaaagttaa gtgatggttc aggcgttgca cattttgctg aaggtattga agttggtcaa
     2641 gtattcaaat tgggtactaa gtattcagaa tcaatgaatg ctacattctt agataaccaa
     2701 ggaaaagctc aatctttaat tatgggttgt tacggaattg gaatttctag aacgctaagt
     2761 gcgattgttg aacaaaatca cgatgataat ggaattgttt ggcctaaatc agttactccg
     2821 tttgatttac atttaatttc tattaatcct aagaaagatg atcaacgaga actagcagat
     2881 gcactatatg ctgaatttaa tactaaattt gatgtgttgt acgatgatcg tcaggaacgt
     2941 gcaggtgtta aatttaatga tgccgattta attggtttac cactgcgaat tgttgttggt
     3001 aaacgtgcat cggaaggtat tgtagaagtt aaagaacgtt taacaggtga tagcgaagaa
     3061 gttcacattg atgacttaat gactgtcatt acaaataaat atgataactt aaaataatta
     3121 agatcgaatg aattataaga gtaggaaaaa gctgaaagaa atctgatgct tatgtcctgc
     3181 tcttattatt tttgatataa tgattattcg atgaaaaatg actgaagaca tagtataatt
     3241 aaagataaat ttgttttaac aatataatga ttagccaaat ataaagcatt taattttcta
     3301 tcattactat gctcacataa tctaaatatt gttcgaacac gtaaaagtaa tttctattta
     3361 aggtggtaat tgtcttggca atgacagagc aacaaaaatt taaagtgctt gctgatcaaa
     3421 ttaaaatttc aaatcaatta gatgctgaaa ttttaaattc aggtgaactg acacgtatag
     3481 atgtttctaa caaaaacaga acatgggaat ttcatattac attaccacaa ttcttagctc
     3541 atgaagatta tttattattt ataaatgcaa tagagcaaga gtttaaagat atcgccaacg
     3601 ttacatgtcg ttttacggta acaaatggca cgaatcaaga tgaacatgca attaaatact
     3661 ttgggcactg tattgaccaa acagctttat ctccaaaagt taaaggtcaa ttgaaacaga
     3721 aaaagcttat tatgtctgga aaagtattaa aagtaatggt atcaaatgac attgaacgta
     3781 atcattttga taaggcatgt aatggaagtc ttatcaaagc gtttagaaat tgtggttttg
     3841 atatcgataa aatcatattc gaaacaaatg ataatgatca agaacaaaac ttagcttctt
     3901 tagaagcaca tattcaagaa gaagacgaac aaagtgcacg attggcaaca gagaaacttg
     3961 aaaaaatgaa agctgaaaaa gcgaaacaac aagataacaa cgaaagtgct gtcgataagt
     4021 gtcaaattgg taagccgatt caaattgaaa atattaaacc aattgaatct attattgagg
     4081 aagagtttaa agttgcaata gagggtgtca tttttgatat aaacttaaaa gaacttaaaa
     4141 gtggtcgcca tatcgtagaa attaaagtga ctgactatac ggactcttta gttttaaaaa
     4201 tgtttactcg taaaaacaaa gatgatttag aacattttaa agcgctaagt gttggtaaat
     4261 gggttagggc tcaaggtcgt attgaagaag atacatttat tagagattta gttatgatga
     4321 tgtctgatat tgaagagatt aaaaaagcga caaaaaaaga taaggctgaa gaaaagcgtg
     4381 tagaattcca cttgcatact gcaatgagcc aaatggatgg tatacccaat attggtgcgt
     4441 atgttaaaca ggcagcagac tggggacatc cagccattgc ggttacagac cataatgttg
     4501 tgcaagcatt tccagatgct cacgcagcag cggaaaaaca tggcattaaa atgatatacg
     4561 gtatggaagg tatgttagtt gatgatggtg ttccgattgc atacaaacca caagatgtcg
     4621 tattaaaaga tgctacttat gttgtgttcg acgttgagac aactggttta tcaaatcagt
     4681 atgataaaat catcgagctt gcagctgtga aagttcataa cggtgaaatc atcgataagt
     4741 ttgaaaggtt tagtaatccg catgaacgat tatcggaaac gattatcaat ttgacgcata
     4801 ttactgatga tatgttagta gatgcccctg agattgaaga agtacttaca gagtttaaag
     4861 aatgggttgg cgatgcgata ttcgtagcgc ataatgcttc gtttgatatg ggcttcatcg
     4921 atacgggata tgaacgtctt gggtttggac catcaacgaa tggtgttatc gatactttag
     4981 aattatctcg tacgattaat actgaatatg gtaaacatgg tttgaatttc ttggctaaaa
     5041 aatatggcgt agaattaacg caacatcacc gtgccattta tgatacagaa gcaacagctt
     5101 acattttcat aaaaatggtt caacaaatga aagaattagg cgtattaaat cataacgaaa
     5161 tcaacaaaaa actcagtaat gaagatgcat ataaacgtgc aagacctagt catgtcacat
     5221 taattgtaca aaaccaacaa ggtcttaaaa atctatttaa aattgtaagt gcatcattgg
     5281 tgaagtattt ctaccgtaca cctcgaattc cacgttcatt gttagatgaa tatcgtgagg
     5341 gattattggt aggtacagcg tgtgatgaag gtgaattatt tacggcagtt atgcagaagg
     5401 accagagtca agttgaaaaa attgccaaat attatgattt tattgaaatt caaccaccgg
     5461 cactttatca agatttaatt gatagagagc ttattagaga tactgaaaca ttacatgaaa
     5521 tttatcaacg tttaatacat gcaggtgaca cagcgggtat acctgttatt gcgacaggaa
     5581 atgcacacta tttgtttgaa catgatggta tcgcacgtaa aattttaata gcatcacaac
     5641 ccggcaatcc acttaatcgc tcaactttac cggaagcaca ttttagaact acagatgaaa
     5701 tgttaaacga gtttcatttt ttaggtgaag aaaaagcgca tgaaattgtt gtgaaaaata
     5761 caaacgaatt agcagatcga attgaacgtg ttgttcctat taaagatgaa ttatacacac
     5821 cgcgtatgga aggtgctaac gaagaaatta gagaactaag ttatgcaaat gcgcgtaaac
     5881 tgtatggtga agacctgcct caaatcgtaa ttgatcgatt agaaaaagaa ttaaaaagta
     5941 ttatcggtaa tggatttgcg gtaatttact taatttcgca acgtttagtt aaaaaatcat
     6001 tagatgatgg atacttagtt ggttcccgtg gttcagtagg ttctagtttt gtagcgacaa
     6061 tgactgagat tactgaagta aacccgttac cgccacacta tatttgtccg aactgtaaaa
     6121 cgagtgaatt tttcaatgat ggttcagtag gatcaggatt tgatttacct gataagacgt
     6181 gtgaaacttg tggagcgcca cttattaaag aaggacaaga tattccgttt gaaacatttt
     6241 taggatttaa gggagataaa gttcctgata tcgacttaaa ctttagtggt gaatatcaac
     6301 cgaatgccca taactacaca aaagtattat ttggtgagga taaagtattc cgtgcaggta
     6361 caattggtac tgttgctgaa aagactgctt ttggttatgt taaaggttat ttgaatgatc
     6421 aaggtatcca caaaagaggt gctgaaatag atcgactcgt taaaggatgt acaggtgtta
     6481 aacgtacaac tggacagcat ccagggggta ttattgtagt acctgattac atggatattt
     6541 atgattttac gccgatacaa tatcctgccg atgatcaaaa ttcagcatgg atgacgacac
     6601 attttgattt ccattctatt catgataatg tattaaaact tgatatactt ggacacgatg
     6661 atccaacaat gattcgtatg cttcaagatt tatcaggaat tgatccaaaa acaatacctg
     6721 tagatgataa agaagttatg cagatattta gtacacctga aagtttgggt gttactgaag
     6781 atgaaatttt atgtaaaaca ggtacatttg gggtaccaga attcggtaca ggattcgtgc
     6841 gtcaaatgtt agaagataca aagccaacaa cattttctga attagttcaa atctcaggat
     6901 tatctcatgg tacagatgtg tggttaggca atgctcaaga attaattaaa accggtatat
     6961 gtgatttatc aagtgtaatt ggttgtcgtg atgatatcat ggtttattta atgtatgctg
     7021 gtttagaacc atcaatggct tttaaaataa tggagtcagt acgtaaaggt aaaggtttaa
     7081 ctgaagaaat gattgaaacg atgaaagaaa atgaagtgcc agattggtat ttagattcat
     7141 gtcttaaaat taagtacatg ttccctaaag cccatgcagc agcatacgtt ttaatggcag
     7201 tacgtatcgc atatttcaaa gtacatcatc cactttatta ctatgcatct tactttacaa
     7261 ttcgtgcgtc agactttgat ttaatcacga tgattaaaga taaaacaagc attcgaaata
     7321 ctgtaaaaga catgtattct cgctatatgg atctaggtaa aaaagaaaaa gacgtattaa
     7381 cagtcttgga aattatgaat gaaatggcgc atcgaggtta tcgaatgcaa ccgattagtt
     7441 tagaaaagag tcaggcgttc gaatttatca ttgaaggcga tacacttatt ccgccgttca
     7501 tatcagtgcc tgggcttggc gaaaacgttg cgaaacgaat tgttgaagct cgtgacgatg
     7561 gcccattttt atcaaaagaa gatttaaaca aaaaagctgg attatctcag aaaattattg
     7621 agtatttaga tgagttaggc tcattaccga atttaccaga taaagctcaa ctttcgatat
     7681 ttgatatgta aaatgaaata atcaaggtat ttatttaatg cgtatggcgt agtcaaagaa
     7741 atacaaaatt gttgctggac acaaaattat gcccgtattt cttttcaatg tcttacgagt
     7801 ctattcaaat gtaatggtga aataaaggaa caaactttta caagaatctc tgattaatag
     7861 tgaagtcatt tgtttcaagc ataaacttat gctataatta agttgcttaa aaattagtga
     7921 actcaggcag aagagtggga gattcccgct cttttctatt tgccaaaaag ggaggcctgt
     7981 atgagtaaaa ttacagaaca agtagaagtg attgttaaac caattatgga agacttgaat
     8041 tttgaacttg tagacgttga atatgtcaaa gagggtagag atcattttct tagaatctct
     8101 attgataaag aaggtggcgt agatttaaat gattgtacgc tagcttctga aaaaataagt
     8161 gaagctatgg atgcaaatga tcctattcct gaaatgtatt atttagacgt agcgtcacct
     8221 ggtgcagaac gtccaattaa aaaagaacaa gatttccaaa atgcaataac taaacctgta
     8281 tttgtttctt tatatgtacc aattgaaggt gaaaaggaat ggttaggcat tttacaagaa
     8341 gtcaataatg aaacaattgt agtacaagtt aaaatcaaag caagaacgaa agatatagag
     8401 ataccgagag acaaaatagc aaaagcacgt cacgcagtta tgatttaacg tgatgaggag
     8461 gaaaaaacgt gtcaagtaat gaattattat tagctactga gtatttagaa aaagaaaaga
     8521 agattcctag agcagtatta attgatgcta ttgaagcagc tttaattact gcatacaaaa
     8581 agaattatga tagtgcaaga aatgtccgtg tggaattaaa tatggatcaa ggtactttca
     8641 aagttatcgc tcgtaaagat gttgttgaag aagtatttga cgacagagat gaagtggatt
     8701 taagtacagc gcttgttaaa aaccctgcat atgaaattgg tgatatatac gaagaagatg
     8761 taacacctaa agattttggt cgtgtaggtg ctcaagcagc gaaacaagca gtaatgcaac
     8821 gtcttcgtga tgctgaacgt gaaattttat ttgaagaatt tatagacaaa gaagaagaca
     8881 tacttactgg aattattgac cgtgttgacc atcgttatgt atatgtgaat ttaggtcgta
     8941 tcgaagctgt tttatctgaa gcagaaagaa gtcctaacga aaaatatatt cctaacgaac
     9001 gtatcaaagt atatgttaac aaagtggaac aaacgacaaa aggtcctcaa atctatgttt
     9061 ctcgtagcca tccaggttta ttaaaacgtt tatttgaaca agaagttcca gaaatttacg
     9121 atggtactgt aattgttaaa tcagtagcac gtgaagctgg cgatcgctct aaaattagtg
     9181 tcttctctga aaacaatgat atagatgctg ttggtgcatg tgttggtgct aaaggcgcac
     9241 gtgttgaagc tgttgttgaa gagctaggtg gtgaaaaaat cgacatcgtt caatggaatg
     9301 aagatccaaa agtatttgta aaaaatgctt taagcccttc tcaagtttta gaagttattg
     9361 ttgatgaaac aaatcaatct acagtagttg ttgttcctga ttatcaattg tcattagcga
     9421 ttggtaaaag aggacaaaac gcacgtctag ctgctaaatt aaccggctgg aaaattgata
     9481 ttaaatcaga aacagatgcg cgtgaagcgg gtatctatcc agtagttgaa gctgaaaaag
     9541 taactgaaga agatgttgct ttagaagatg ctgacacaac agaatcaacc gaagaggtaa
     9601 atgatgtttc agttgaaaca aatgtagaga aagaatctga ataataggtt ggagtgaagt
     9661 atctatgaaa aagaaaaaaa ttccgatgcg aaaatgtatt ctttcaaatg aaatgcatcc
     9721 caaaaaagat atgattcgtg ttgttgttaa taaagaaggc gaaatctttg cggatgttac
     9781 tggaaagaaa caaggccgtg gcgcatatgt ttctaaagat gttgctatgg ttgaaaaagc
     9841 acaacaaaaa gaaattttag aaaaatattt taaagcatct aaagagcaat tggatcctgt
     9901 ttacaaagaa attattagat taatttatag agaagagatc ccaaaatgag tatagatcaa
     9961 atattaaact ttttaggatt agcaatgaga gctggtaaag taaaaacagg tgaatcagtc
    10021 attgttaatg agattaaaaa aggaaatttg aagctcgtta ttgttgcaaa tgatgcgtct
    10081 gataatacag ctaaattaat tacagataaa tgtaagagtt acaaagttcc attcagaaag
    10141 tttggaaatc gaaatgaatt gggaatagca cttggaaaag gtgagcgtgt taatgtaggg
    10201 attactgacc caggctttgc taaaaagttg ctatcaatga tagatgaata tcataaggag
    10261 tgattatatg agtaaacaaa gaatttacga atatgcgaaa gaattaaatc taaagagtaa
    10321 agagattata gatgagttaa aaagcatgaa tattgaggtt tcaaatcata tgcaagcttt
    10381 ggaagatgac caaattaaag cattagataa aaagttcaaa aaagaacaaa agaacgacaa
    10441 taaacaaagc actcaaaata atcaccaaaa atcaaacaat caaaaccaaa ataaagggca
    10501 acaaaaagat aacaaaaaga atcaacaaca aaataataaa ggcaacaaag gcaataaaaa
    10561 gaataataga aataataaga aaaataacaa gaataataaa ccacaaaatc aaccagctgc
    10621 tccaaaagaa ataccatcaa aagtgacata tcaagaaggt attacagtag gcgaatttgc
    10681 ggataaatta aatgttgaat catcagaaat tatcaaaaaa ttattcttac ttggtattgt
    10741 tgctaatatc aatcaatcat taaatcaaga aacaatcgaa ttaattgccg atgattatgg
    10801 cgttgaggtt gaagaagaag ttgtgattaa tgaagaagac ttatcaatct atttcgaaga
    10861 cgaaaaagat gatccagagg caattgagag accagcagtt gtaacaatta tgggacatgt
    10921 tgaccatggt aaaacgactt tattagattc aattcgtcat acaaaagtta cagcaggtga
    10981 agcaggcgga atcactcaac atattggtgc atatcaaatt gaaaacgatg gcaaaaaaat
    11041 cactttctta gatacaccgg gacatgctgc atttacaacg atgcgtgcgc gtggtgcaca
    11101 agtaacagat attactattt tagtagtagc agctgacgat ggtgttatgc cacaaacaat
    11161 tgaagcaatt aaccatgcta aagaagcaga agtaccaatt attgttgcag taaataaaat
    11221 tgataaacca acttcaaatc ctgatcgagt tatgcaagaa ttaactgaat atggtttaat
    11281 tcctgaagat tggggcggcg aaacaatttt cgttccactt tctgcattaa gtggtgatgg
    11341 tatcgacgat ttattagaaa tgataggatt agttgcagaa gttcaagaac ttaaagcaaa
    11401 tcctaaaaac cgtgctgttg gtacagttat cgaagctgaa ttagataaat cacgtggtcc
    11461 ttctgcatca ttattagtac aaaacggtac attaaatgtt ggtgatgcga ttgtagttgg
    11521 taatacttac ggccgtatcc gtgcaatggt taatgactta ggtcaaagaa tcaaaacggc
    11581 tggtccatca acgcctgttg aaattacagg tattaatgat gtgccacaag ctggggatcg
    11641 ctttgttgta tttagtgatg aaaaacaagc tcgtcgtatt ggtgaatcaa gacacgaagc
    11701 tagcattata caacaacgtc aagaaagtaa aaatgtttca ttagataacc tgtttgaaca
    11761 aatgaaacaa ggtgaaatga aagatttaaa cgttattatt aaaggtgatg ttcaaggttc
    11821 tgttgaagct ttagctgcat cattaatgaa aattgatgtt gaaggcgtaa atgttcgtat
    11881 cattcataca gcggttggtg caattaatga gtcagacgtg acacttgcta atgcctcaaa
    11941 tggtattatc attggtttca atgttcgtcc agacagtggt gcaaaacgtg ctgcagaagc
    12001 tgaaaatgtt gatatgcgtt tacacagagt tatttataat gttatcgaag aaattgaatc
    12061 agcgatgaaa ggtttacttg atccagaatt tgaagaacaa gttatcggac aagctgaagt
    12121 tcgtcaaaca ttcaaagttt ctaaagttgg tactattgct ggatgttatg ttactgaagg
    12181 taaaattacg cgaaatgctg gtgtacgtat tattcgtgat ggtattgttc aatatgaagg
    12241 cgaattagat acacttaaac gtttcaaaga tgatgctaag gaagttgcaa aaggttatga
    12301 atgtggtatt acaattgaaa actacaatga ccttaaagaa ggcgatgtta tcgaagcatt
    12361 tgaaatggtt gaaattaagc gttaattaaa taaattacaa gctaaaagta tagttaagat
    12421 tgatatgctc cctataaata ttgcactttt taagtgtcta ctttataggg agcatatttg
    12481 atactagctt ttggtttttt attagaatag attacctatt aaaagttacg ttatatggac
    12541 atgattttgt ataaaatttt gtggtggcct agaatgattt ttaatgacaa aatataatgt
    12601 cgactattat tggaaaattt tctgttgaaa tgcctatctt acggcaaact ttatttgatt
    12661 ttataggctt aatttattaa aataacgtgt gagctaaaat aattgtttaa gcattgttac
    12721 actaaaaaat gcaaataaca attgaactta aagataaaga ggtgacaaga atgagcagta
    12781 tgagagcaga gcgtgttggt gaacaaatga agaaggaatt aatggatatc atcaacaata
    12841 aagtcaaaga tcctcgagtt ggttttatta caattacaga tgttgtttta acaaatgatt
    12901 tatcgcaggc taaagtattt ttaactgtat taggtaacga taaagaagta gaaaatacat
    12961 ttaaagcact tgataaagca aaaggcttca ttaagtctga attaggttct agaatgcgat
    13021 tacgtattat gccggaatta atgtatgaat atgatcaatc aatcgaatat ggtaataaaa
    13081 ttgaacgaat gattcaagat ttacacaaac aagatagata atttagtgtt aggtatctgg
    13141 aaaatgtttg ataatttctt aatatcggta tattaacatt aaacagttaa tacatagatg
    13201 tgtagaaata gttaacattt tccagttttt ttatgaataa atttagttga tacgctatta
    13261 aaatatattt taaaaaagaa ggtgactata tgtataatgg gatattacca gtatataaag
    13321 agcgcggttt aacaagtcat gacgttgtat tcaaattgcg taaaatatta aaaactaaaa
    13381 aaataggtca cacgggtacg cttgatcccg aagttgcagg cgtgttaccg gtatgtatag
    13441 gtaatgcaac gagagttagt gattatgtta tggatatggg caaagcttat gaagcaactg
    13501 tatcgatagg aagaagtaca acgactgaag atcaaacggg tgatacattg gaaacaaaag
    13561 gtgtacactc agcagatttt aataaggacg atattgaccg attgttagaa agttttaaag
    13621 gtatcattga acaaattccg ccgatgtact catccgtcaa agtaaatggt aaaaaattat
    13681 atgaatatgc gcgtaataat gaaacagttg aaagaccaaa gcgtaaagtt aatattaaag
    13741 acattgggcg tatatctgaa ttagatttta aagaaaatga gtgtcatttt aaaatacgcg
    13801 tcatctgtgg taaaggtaca tatattagaa cgctagcaac tgatattggt gtgaaattag
    13861 gctttccggc acatatgtcg aaattaacac gaatcgagtc tggtggattt gtgttgaaag
    13921 atagccttac attagaacaa ataaaagaac ttcatgagca ggattcattg caaaataaat
    13981 tgtttccttt agaatatgga ttaaagggtt tgccaagcat taaaattaaa gattcgcaca
    14041 taaaaaaacg tattttaaat gggcagaaat ttaataaaaa tgaatttgat aacaaaatta
    14101 aagaccaaat tgtatttatt gatgatgatt cagaaaaagt attagcaatt tatatggtac
    14161 accctacaaa agaatcagaa attaaaccta aaaaagtctt taattaaagg agatagaatt
    14221 tatgaaagtc atagaagtga cacatcctat acaatctaaa cagtatatta cagaggatgt
    14281 tgcaatggca ttcggatttt tcgatggcat gcataaaggt catgacaaag tctttgatat
    14341 attaaacgaa atagctgagg cacgcagttt aaaaaaagcg gtgatgacat ttgatccgca
    14401 tccgtctgtc gtgttgaatc ctaaaagaaa acgaacaacg tatttaacgc cactttcaga
    14461 taaaatcgaa aaaattagcc aacatgatat tgattattgt atagtggtta atttttcatc
    14521 taggtttgct aatgtgagcg tagaagattt tgttgaaaat tatataatta aaaataatgt
    14581 aaaagaagtc attgctggtt ttgattttac ttttggtaaa tttggaaaag gtaatatgac
    14641 tgtacttcaa gaatatgatg cgtttaatac gacaattgtg agtaaacaag aaattgaaaa
    14701 tgaaaaaatt tctacaactt ctattcgtca agatttaatc aatggtgagt tgcaaaaagc
    14761 gaatgatgct ttaggctata tatattctat taaaggcact gtagtgcaag gtgaaaaaag
    14821 gggaagaact attggcttcc caacagctaa cattcaacct agtgatgatt atttgttacc
    14881 tcgtaaaggt gtttatgctg ttagtattga aatcggcact gaaaataaat tatatcgagg
    14941 ggtagctaac ataggtgtaa agccaacatt tcatgatcct aacaaagcag aagttgtcat
    15001 cgaagtgaat atctttgact ttgaggataa tatttatggt gaacgagtga ccgtgaattg
    15061 gcatcatttc ttacgtcctg agattaaatt tgatggtatc gacccattag ttaaacaaat
    15121 gaacgatgat aaatcgcgtg ctaaatattt attagcagtt gattttggtg atgaagtagc
    15181 ttataatatc tagagttgcg tatagttata taaacaatct ataccacacc tttttcttag
    15241 taggtcgaat ctccaacgcc taactcggat taaggagtat tcaaacattt taaggaggaa
    15301 attgattatg gcaatttcac aagaacgtaa aaacgaaatc attaaagaat accgtgtaca
    15361 cgaaactgat actggttcac cagaagtaca aatcgctgta cttactgcag aaatcaacgc
    15421 agtaaacgaa cacttacgta cacacaaaaa agaccaccat tcacgtcgtg gattattaaa
    15481 aatggtaggt cgtcgtagac atttattaaa ctacttacgt agtaaagata ttcaacgtta
    15541 ccgtgaatta attaaatcac ttggtatccg tcgttaatct taatataacg tctttgaggt
    15601 tggggcatat ttatgttcca accttaattt atattaaaaa agctttttac aaatattaac
    15661 atttattata tgttaagcta atattgagtg aataataagg ttacaatgag ataaagatga
    15721 tataagtaca cctagagtaa taatcaagat attaaaaata aagtatgttt ttttaaaaaa
    15781 tataacttat atttatactg ataagggtgg gacgataagt ctattttgta aataatagat
    15841 ggatatcccg ctctcttttt ttccaattca atattttata actaatatta aaatacgata
    15901 ataaatgata tgatataact attagattca agagaggaga tttataatgt ctcaagaaaa
    15961 gaaagttttt aaaactgaat gggcaggaag atctttaacg attgaaacag ggcaattagc
    16021 taaacaagca aatggcgctg tattggttcg ttatggagat acagtcgtgt tatcgacggc
    16081 aactgcatca aaagaacctc gtgatggaga tttcttccca ttaacagtga actatgaaga
    16141 aaaaatgtac gctgcgggta aaattcctgg tggatttaaa aagagagaag gacgtcctgg
    16201 tgacgatgca acattaactg cgcgattaat tgatagacca attagacctt tattccctaa
    16261 aggatataag catgatgttc aaattatgaa catggtatta agtgcagatc ctgattgttc
    16321 accacaaatg gctgcaatga ttggttcatc tatggcgctt agtgtgtcgg atattccatt
    16381 ccaagggcca atcgccggtg taaatgtggg ttatattgac ggtaaatata tcattaaccc
    16441 aacagtagaa gaaaaagaag tttctcgttt agaccttgaa gtagctggtc ataaagatgc
    16501 ggtaaacatg gtagaggcag gcgctagtga gattactgaa caagaaatgt tagaggcgat
    16561 tttctttggt catgaagaga ttcaacgttt agttgatttc caacaacaaa tcgtcgacca
    16621 cattcaacct gttaaacaag aatttattcc agcagagcgt gatgaagcgc tagttgaacg
    16681 tgtaaaatct ttaaccgaag aaaaaggact taaagaaaca gttttaacat ttgataaaca
    16741 acaacgagat gaaaatcttg ataacttaaa agaagaaatc gtcaatgaat ttatcgatga
    16801 agaagatcca gagaatgaat tacttattaa agaagtttat gcaattttaa atgaattagt
    16861 gaaagaagaa gttcgacgtt taattgcaga tgaaaaaatt agaccagacg gccgtaaacc
    16921 tgatgaaatc cgtccattag attctgaagt tggtatttta cctagaacgc atggttcagg
    16981 tctatttaca cgtggtcaga ctcaagcact ttcagtttta acattaggtg ctttaggcga
    17041 ttatcaatta attgatggtt taggacctga agaagaaaaa agattcatgc atcattacaa
    17101 cttcccgaat ttttcagtag gtgaaactgg tccagtacgt gcgccaggtc gtcgtgaaat
    17161 tggacatggt gcgttaggtg aaagagcatt aaaatatatt attcctgata ctgctgattt
    17221 cccatataca attcgtattg taagtgaggt acttgaatca aatggttcat catctcaagc
    17281 gtcaatttgt ggatcaacat tagcattaat ggatgcgggc gtaccgatta aagcaccagt
    17341 tgctggtatt gctatgggcc ttgttacacg tgaagatagc tatacgattt taactgatat
    17401 ccaaggtatg gaagatgcat taggtgatat ggactttaaa gtcgctggta ctaaagaagg
    17461 tattacagca atccaaatgg atattaaaat tgacggttta acgcgtgaaa ttatcgaaga
    17521 ggctctagaa caagcgagac gtggtcgttt agaaataatg aatcatatgt tacaaacaat
    17581 tgatcaacca cgtactgaat taagtgctta cgcgccaaaa gttgtaacta tgacaattaa
    17641 accagataag attagagatg ttatcggacc tggtggtaaa aaaattaacg aaattattga
    17701 tgaaacaggt gttaaattag atattgaaca agatggtact atctttattg gtgctgttga
    17761 tcaagctatg ataaatcgtg ctcgtgaaat cattgaggaa attacacgtg aagcggaagt
    17821 aggtcaaact tatcaagcca ctgttaaacg tattgaaaaa tacggtgcgt ttgtaggcct
    17881 attcccaggt aaagatgcgt tgcttcacat ttcacaaatt tcaaaaaata gaattgaaaa
    17941 agtggaagat gtattaaaaa tcggtgacac aattgaagtt aagattactg aaattgataa
    18001 acaaggtcga gtaaatgctt cacatagagc attagaagaa taatatttaa agtcatatga
    18061 cgacaatgta tcgtcatgtg atttttttat gccacttttt acgaagtgac ccgttttgaa
    18121 tttgttgtat tgaacatttt aaaacgcttt attattttgt gtgcaactgt taattatcct
    18181 gtatgtatag tgattaatag tgtacatcaa gtgtttttta acttataatg aatagtgagt
    18241 ttatatatgg acgggtaaca aatttaggag gtaagatttt gagtttaata aagaaaaaga
    18301 ataaagatat tcgcattata ccattaggcg gtgttggcga aattgctaaa aatatgtata
    18361 tcgttgaagt agacgatgaa atgtttatgt tagatgctgg acttatgttt ccagaagacg
    18421 aaatgctagg tattgatatt gttataccag acatttcata cgtacttgaa aataaagata
    18481 aattgaaggg tatattcctt acacacggac atgagcacgc gattggtgca gtgagttatg
    18541 ttttagaaca attagatgca ccagtatatg gatctaaatt gacaatagcg ttaattaaag
    18601 aaaatatgaa agcccgtaat attgataaaa aagttcgcta ctatacagtt aataatgatt
    18661 caattatgag attcaaaaac gtgaatatta gtttctttaa tacgacacac agtattcctg
    18721 atagtttagg tgtttgtatt cacacttcat atggtgccat tgtgtataca ggtgaattta
    18781 agtttgacca aagtttacat ggacattatg caccagatat taaacgtatg gcagagattg
    18841 gtgaagaagg cgtatttgtc ttaatcagtg attctactga ggcagagaaa cctggatata
    18901 atactccgga aaatgtgatt gaacatcata tgtatgatgc ttttgcaaaa gtgcgaggtc
    18961 gcttgatagt ttcatgttat gcttcgaact ttatacgtat tcagcaagtt ttaaatattg
    19021 ctagcaagct aaatcgtaaa gtgtcatttt taggaagatc acttgaaagt tcatttaata
    19081 ttgctcgtaa aatggggtat ttcgacattc ctaaagattt gctaattcct ataacagaag
    19141 ttgataatta tcctaaaaat gaagtgataa ttatagctac tggtatgcaa ggagaacctg
    19201 tagaagcctt aagtcaaatg gcgcaacata agcataaaat tatgaatatc gaagaaggcg
    19261 attctgtatt tttagcaatt acggcttctg ctaatatgga agttatcatt gcgaatacat
    19321 taaatgagct tgtacgtgct ggcgcacata ttattccaaa taacaaaaag attcatgctt
    19381 caagtcatgg ttgcatggaa gaattaaaaa tgatgattaa tattatgaaa cctgaatact
    19441 ttattcctgt acaaggtgaa tttaaaatgc agatagcaca tgcgaagcta gcagctgaag
    19501 caggtgttgc accagaaaag attttccttg tggaaaaagg agatgtcatt aattacaacg
    19561 gtaaagatat gatattaaat gaaaaggtaa attcaggaaa tattttaata gatggcattg
    19621 gtattgggga tgtaggaaat atcgtgttga gagaccgtca tcttttagca gaagatggta
    19681 tctttattgc tgttgtaacg ttagatccta aaaatagacg tatagctgcg ggacctgaaa
    19741 ttcaatctcg tgggtttgta tatgtacgtg aaagtgaaga cttattacgt gaagcagaag
    19801 agaaagtacg tgaaatagta gaggctggtt tacaagaaaa acgcatagaa tggtctgaaa
    19861 ttaaacaaaa tatgcgtgat caaattagta aactattatt cgaaagtaca aaacgtcgtc
    19921 ctatgattat tccagtaatt tctgaaattt aatcaaaaag tcattaacat aaaagaggtc
    19981 agaacaagtc actgaaatat aatggttgtc atggacaatt tacttatatt ttatgatggt
    20041 caattgaagg ggtaacgatt aatctgttat cttaagtaaa ttgatacata gatgatattg
    20101 ttctaacctc tttcatcgtc tgtttggact acatattcta aacatcaaat aggaaattat
    20161 atataataac gtcgttttaa ctaaggcaac ataaggaggt gcgtcaattg gcacaagcaa
    20221 aaaagaaatc gacagctaag aaaaaaacaa catcaaaaaa aagaacaaat tcgaggaaaa
    20281 agaagaatga taatccgata cgttatgtca tagctatttt agtagttgta ttaatggtgt
    20341 tgggtgtttt ccaattagga ataataggtc gtctaattga cagcttcttt aattatttat
    20401 ttgggtacag tagatattta acatatattt tagtactctt agcaactggt tttattacat
    20461 actctaaacg tattcctaaa actagacgaa cggctggttc gattgtattg caaattgcat
    20521 tgctatttgt atcacagtta gtttttcatt ttaatagtgg tatcaaagct gaaagagaac
    20581 ctgtactttc ttatgtgtat cagtcatacc aacacagtca tttcccaaat tttggtggcg
    20641 gtgtattagg cttttattta ttagagttaa gcgtaccttt aatttcatta tttggtgtat
    20701 gtattattac tattttatta ttatgctcaa gtgttatttt attaacaaac catcaacatc
    20761 gtgaagttgc aaaagttgca ctggaaaata taaaagcttg gtttggttca tttaatgaaa
    20821 aaatgtcgga aagaaaccaa gaaaaacaat tgaagcgtga agaaaaagca agacttaaag
    20881 aagaacaaaa ggcacgtcaa aatgaacagc cacaaataaa agatgtgagt gattttacgg
    20941 aagtgcctca agaaagagat attccaattt atgggcatac tgaaaatgaa agtaaaagcc
    21001 agagtcaacc aagtcgaaaa aaacgagtgt ttgatgcaga gaatagttcg aataacatcg
    21061 taaatcatca tcaagcagat cagcaagaac aattaacaga acaaactcat aacagtgttg
    21121 aaagtgaaaa cactattgaa gaagctggtg aagttacgaa tgtatcgtat gttgttccac
    21181 cgttaacttt acttaatcaa cctgcaaaac aaaaagcaac atctaaagct gaagtgcaac
    21241 gtaaaggaca agtactagag aatacattaa aagattttgg ggtaaatgca aaagtgacac
    21301 aaattaaaat tggtcctgca gtaactcaat atgaaattca accagctcaa ggggttaaag
    21361 tgagtaaaat tgtaaacttg cataatgata ttgcattagc tttagcagca aaagatgtta
    21421 gaatcgaagc gccaatacct ggtcgttctg cagtaggtat tgaagtgcca aatgagaaaa
    21481 tttcattagt ttcactaaaa gaagttttag atgaaaaatt cccgtctaat aataaactag
    21541 aagttggatt aggaagagat atatcaggtg atccaattac tgttccacta aatgaaatgc
    21601 cacacttatt ggtggcagga tcgacgggta gtggtaaatc tgtttgtata aatggtatta
    21661 ttacaagtat tttattaaat gctaagccgc atgaagttaa acttatgtta atcgatccga
    21721 aaatggttga actaaatgtt tataacggaa ttccacactt attaattccg gttgttacaa
    21781 atcctcataa agctgctcaa gctttagaaa aaattgtagc tgagatggaa agacgttatg
    21841 atttattcca acattcatca actagaaaca ttaaaggtta taacgaatta atccgtaagc
    21901 aaaatcaaga attagatgag aagcaaccag aattacctta tatcgttgtt attgtagatg
    21961 agcttgcaga tttaatgatg gtagctggta aagaagttga aaatgcgatt caacgtatta
    22021 cacaaatggc acgtgcagca ggtatacatt taattgtagc gacacaaaga ccttctgtgg
    22081 atgtaattac aggtatcatt aaaaataata ttccatctag aatagctttt gctgtgagtt
    22141 ctcaaacaga ttcaagaact attattggta ctggcggcgc agaaaagtta cttggtaaag
    22201 gtgacatgtt atacgttgga aatggtgact catcacaaac acgtattcaa ggggcgtttt
    22261 taagtgacca agaggtgcaa gatgttgtaa attatgtagt agaacaacaa caggcaaatt
    22321 atgtaaaaga aatggaacca gatgcaccag tggataaatc ggaaatgaaa agtgaagatg
    22381 ctttatatga tgaagcgtat ttgtttgttg ttgaacaaca aaaggcaagt acatcattgt
    22441 tacaacgcca atttagaatt ggttataata gagcatctag gttgatggat gatttagaac
    22501 gcaatcaggt aatcggtcca caaaaaggaa gcaagcctag acaagtttta atagatctta
    22561 ataatgacga ggtgtaaaaa aatgtcagaa atgaatgcgg tatataacgt taaacaatac
    22621 attttaaatt tgattaagca aaataaattg gaatatggtg accaacttcc aagtaattta
    22681 tcaattgcca gagaattaaa tgtaaaaacc gacgatgttt atgaagcaat tcaggcattg
    22741 attactgaac aagtcattaa agataatttt gaagagggca caagtgttaa gtcactgccc
    22801 cctttctttt atccattgaa tgaacttata agtattgggc aaatgattaa aaatgcagga
    22861 tttgaatgcg gaactgaata cttaaatttt gatgagcaac cagcaactat gttagatgca
    22921 aatttgttga gcgttgaaga aggatatcca gtaaccatta tagaacgatt acgaactgcc
    22981 gatggagaac cggtcgtcta ttgtttagat aaaattgcta aaaaagaatt aacatgtaca
    23041 gagtatcaaa tgagcaatgg atcgattcta agtgcgataa aagaacaaag taatcataat
    23101 atttgttatg ctgatacaga aattgaagcg gtaaattatg aacctcgaat atccgaagta
    23161 ctgaatgctt cgccacacga aggtttgatt ttattaaaaa ttacgcacta taatgaatca
    23221 gatgaaccta ttttgtattc attaaattat atgaaaaata gcttagttca atttaaaatc
    23281 actagaaaaa tatagatgta agaagatata gaggaggcta tatatttgag tagacaatct
    23341 caaccaaata tacatatcaa agtttcacca acaactaaat ttaaaacaac tactatagtt
    23401 tttaaattta tggcaccttt agaatatgac acaataacag ctagatcatt attaagcaaa
    23461 ttattagttc gagcaactaa gaaatggcca accgataagt cgtttaataa tcacttagcc
    23521 gatttatatg gtgcgtatgt gaatagtaca atttcaaaat tcaaagatca gcatgtcatt
    23581 acattttcat tagaaattgt taatgaacgt tatttaagaa acggtgaatc attatttaat
    23641 caaggattag atttattaca agaaatcatt tggaatccat taattgaaaa taaagcattc
    23701 aatgataatt ttgttaacca agagaaaaca ttattagcca aaaaaataga agcaatggta
    23761 gataataaag cacaatattc gtttttaaaa ttactcgacc atatgtttga aaatgaagca
    23821 tataaatact tatctacagg acaactagaa caaatcccac atattactgc tgaaacacta
    23881 tatcatacat atcaatcaat gattaataat gatcaatgtt ctgtttatgt tgtcggcaat
    23941 gtagaacctg aaagtgttga gaaacaaata cgtgaaaaat ttgcacttaa accattcgat
    24001 aaacatcaat tccaacattc tactcatcat ttacacgatg aagaagttga ttatattgtt
    24061 gaatatgatg acgtggatca agctaaatta aatatgggat accgttttcc aacacaatat
    24121 ggacaaagtg gatatgctgc ctttgttgta tttaacatga tgtttggagg agatccttca
    24181 tctgttttat ttaatgaagt gcgagaaaag caaagtttag cgtactctat acattcacaa
    24241 attgatggca aaaatggcta tttatttgtt ttgagtgggg tttcaagtga taagtacgaa
    24301 actgcaaaag acactattat aagtgaattt gaaaaaataa aagcaggaga tttcactgaa
    24361 gaaaaattag agttagctaa aaaagtaatc atttctcatc gatatgaatc tgaagatcgt
    24421 ccgaaaagta ttatagagat tatgcataac caaatattat tagagcaacc acaaagcaaa
    24481 gaaacattta taaatgatat acagaaggta agtcgcgaag atattgtttc tgttgctgaa
    24541 aaagcatttt tagatacaat ctatgtgttg acaaaaggag gggataaata atgaaagagc
    24601 gttattatga attaatagac gaaagagtat tcgaacaaga attagaaaat ggtttacgat
    24661 tatttattat ccccaaacca ggttttcaaa agacatttgt cacttacact acacaatttg
    24721 gttcattaga taatcaattc aaaccccttg gacaagacca atttgttact gtacctgatg
    24781 gagttgctca ctttttagaa cataaattat ttgaaaaaga agaagaagac ttatttactg
    24841 cgtttgctga agataacgca caagcaaatg cgtttacaag ctttgatcgt acaagctact
    24901 tgttcagtgc aactgataat attgaaaaca acattaaacg tttacttaca atggttgaaa
    24961 cgccttattt tacaaaagaa actgttgata aagaaaaagg tattattgca gaagaaataa
    25021 aaatgtatca agagcaacct ggatataaat taatgtttga tacattgcgt gcaatgtatc
    25081 aacaacatcc aatacgtgtt gatattgccg gtagtgtaga aagtatatac gatattacaa
    25141 aagatgattt gtatctatgt tatgaaacgt tttatcatcc atcaaatatg gttttatttg
    25201 ttgttggcga tgtggatcct gaagcaatat gtcgaatagt aaaacaacac gaggatgctc
    25261 gtaataaagt taaccaaccc aaaatcgaac gaggacttgt tgatgaaccg gaggatgtta
    25321 aagaagcatt tgttactgaa tctatgaaaa ttcaatcacc aagactaatg cttggtttta
    25381 aaaataaacc attacaagaa gcgcctcaaa aatatgtaca acgtgattta gaaatgtcat
    25441 tattctttga gttaattttt ggggaagaaa cagattttta tcagaattta ttaaacgaag
    25501 gacttatcga tgatacattt ggttatcaat ttgtactaga gccgacgtat agtttttcaa
    25561 tcgtgacaag tgctactgaa gaaccagata aattaaaaaa attattatta gatgagttgc
    25621 gtgataaaaa aggcaatttc caagatgcag aagcatttga acttttgaaa aagcaattta
    25681 taggtgaatt catatcaagt ttaaactcac ctgaatatat tgctaatcaa tatactaaat
    25741 tgtattttga aggtgttagc gtgtttgaca tgttagacat tgttgaaaat atcacgttag
    25801 atagtattaa cgaaacgtca tcgttatatt taaatttaga tcagcaagtc gatagtcgtt
    25861 tggagattaa aaagtaatga aagcattagt attaggtggt tctggttcaa ttggttctga
    25921 gatagtcaaa caattattaa ctgatggatt tgaagtttat gtgcaatatt atcgtactga
    25981 tataaatgaa ttaactagca aatttaatga tgataaagtt cgttttatac aagcggattt
    26041 atctcaaaca attgatattg acaaaacatt tggtgacatt aaatcattag actgtttaat
    26101 atatgcaagt ggtcagtctt tatatggtgt tttacaagat atgaaagacc atgatattga
    26161 tgcatgttat cagttaaatg tcttgcaatt aattcgatta tgtagatatt tcgttgatgt
    26221 tttacgtcaa agtgccaatg gaagaattat tgtaatttca tcaatttggg gtgagacagg
    26281 agctagtatg gaaactattt attcggcgat gaaaagtgca caattaggtt tcgttaaggc
    26341 gcttagtcaa gagcttgcac taacatcagt gacagtaaat gctatcgcac ctggatttgt
    26401 agccggtaat atggcaagtg agtggcaaga agatgaactt caagcaatga taactgaatt
    26461 accacaacag cgattggttt taccgagtga ggttgctcat acatgcgcct atttatatca
    26521 cccaaatgct agaagtgtta ctggaactat acagaaagtt aatggtgctt ggtatattta
    26581 aaatatagaa ctatttttat gagcaaatgt ttttatgatt gatgttataa tatgaatatt
    26641 acaatgaatc ataatttgtt aattaaacaa ggggtgaggt tcaccatgac agttgcagag
    26701 aaaaaagaat ggtacctaga atacgaaatt gaaattaata gaccgggtct tttaggtgat
    26761 gtatctagtt tattaggtat gttaggtata agtattgtta caattaatgg tgttgatcaa
    26821 ggtaaacgag gccttttaat taaaacagac aatcttgaaa aagttgaacg ttttgagcaa
    26881 atagctcgtg gtataaatga aattgaaata acaaagctta aaaaaccaga attaagagac
    26941 cgtcttgcag taagacatgg aagatatatt gagcaagatg caaaagataa gaaaaccttt
    27001 cgatttgagc gtgaagattt aggcttgtta gtagactttt tagctgaatt gttcaaagaa
    27061 gaaggtcata agttgattgg cattagaggt atgccacggg ttggtaaaac tgaatcaatt
    27121 gttgcgggaa gtgtttgtgc acataagaga tggttattta ttagttctac tttaataaaa
    27181 caaactgtac gtagctctct aattaaaggg gaatatgatg ccaatcatgt atacattatt
    27241 gatggtgcag ttactgccag agaatctaat ccaaaacatc aagagcttgt taacgaagtt
    27301 atgacgttac catcaatcaa agtcgttgaa catccagatt tatttgttga aacaagtact
    27361 tgtacaatgg aagattttga ctatattatc gaattgagag aaaatgaaaa tcaagaaata
    27421 cattacgaag aaatgaagaa acagacagtc caaagtaaga ataatttaga ttttggagat
    27481 ccgtttggtg gtggttttgg tttcttcgag taagtgtaag gaggctatga attgaaaacg
    27541 gtcggtgaag cgctaaaagg tagacgtgaa aggttaggaa tgattttaac agaattagag
    27601 caacgtactg gaattaaacg tgaaatgcta gtgcatattg aaaataatga attcgatcaa
    27661 ctaccgaata aaaattacag cgaaggattt attagaaaat atgcaagcgt agtaaatatt
    27721 gaacctaacc aattaattca agctcatcaa gatgaaattc catcgaacca agccgaatgg
    27781 gacgaagtaa ttacagtttt caataataat aaagacttag attataagag taaatcaaaa
    27841 gagccaatac aattattagt aatcatgggt attacagttt taataacttt attgttatgg
    27901 atcatgttag ttttaatatt ttaacagaaa taaattagtg agaaatgagg atgtataatg
    27961 aatattccga accagattac ggtttttaga gtagtgttaa taccagtttt tatattgttt
    28021 gcgttagttg attttggatt tggcaatgtg tcatttctag gaggatatga aataagaatt
    28081 gagttattaa tcagtggttt tatttttata ttggcttccc ttagcgattt tgttgatggt
    28141 tatttagcta gaaaatggaa tttagttaca aatatgggga aatttttgga tccattagcg
    28201 gataaattat tagttgcaag tgctttaatt gtacttgtgc aactaggact aacaaattct
    28261 gtagtagcaa tcattattat tgccagagaa tttgccgtaa ctggtttacg tttactacaa
    28321 attgaacaag gattcgtaag tgcagctggt caattaggta aaattaaaac agcagttact
    28381 atggtagcaa ttacttggtt gttattaggt gatccattgg caacattgat tggtttgtca
    28441 ttaggacaaa ttttattata cattggcgtt atttttacta tcttatctgg tattgaatac
    28501 ttttataaag gtagagatgt ttttaaacaa aaataaatat ttgtttatac tagatttcat
    28561 tttcatatgg aatctagttt ttttaatccc aattttagaa attagccacg caattgttta
    28621 taatgatata ttgtaaaaca atatttgttc atttttttag ggaaaatctg tagtagcatc
    28681 tgatacattg aatctaaaat tgatgtgaat ttttaaatga aatacatgaa aaaatgaatt
    28741 aaacgataca agggggatat aaatgtcaat tgccattatt gctgtaggct cagaactatt
    28801 gctaggtcaa atcgctaata ccaacggaca atttctatct aaagtattta atgaaattgg
    28861 acaaaatgta ttagaacata aagttattgg agataataaa aaacgtttag aatcaagtgt
    28921 acgtcatgcg ctagaaaaat atgatactgt tattttaaca ggtggcttag gtcctacgaa
    28981 agatgactta acgaagcata cagtggccca gattgttggt aaagatttag ttattgatga
    29041 gccttcttta aaatatattg aaagctattt tgaggaacaa ggacaagaaa tgacacctaa
    29101 taataaacaa caggctttag taattgaagg ttcaactgta ttaacaaatc atcatggcat
    29161 ggctccagga atgatggtga attttgaaaa caaacaaatt attttattac caggtccacc
    29221 gaaagaaatg caaccaatgg tgaaaaatga attgttgtca cattttataa accataatcg
    29281 aattatacat tctgaactat taagatttgc gggaataggt gaatctaaag tagaaacaat
    29341 attaatagat cttatcgata aacagactaa tcctacgatt gcgcctttgg cgggaagtca
    29401 tgaagtatat attagattga ctgcaaatgc cgactcaaaa gaacaagcac aatcattgat
    29461 tcaacctgtt aaacaagaaa ttcttgatcg tattggagaa tattattatg gttcagatga
    29521 cacattaatt gagcaagctg taataaagaa aattcatgaa ccttttgtaa tatatgatgg
    29581 tattactaat ggtgctttat atcatcgatt gaaagaagtg gatttaaacg atgttctaaa
    29641 gggtatgatt aatcacaatg aaaactttgt tgatattaat aaacctattg agcagcaatt
    29701 aaaagatgca gtgcaatttg ttaataaatt gtttaatgtg tcatcagcaa ttattctatt
    29761 agagtatgat ggtgtagttc atataggcta tgataataac tttgaattta aaactgagca
    29821 atttaaaatg tctaaatcta gaaatttatt aaagaacaga agtcaaaatt atgcgctcat
    29881 aagattatta aattggctta gaacaacaaa ttaattgtat tatcgataaa aatataagca
    29941 cgtttgttcg tttttgcgtt ttgatttcaa gattttatac gaacaaatat tcgcaaaaca
    30001 cttgtatttt attttgaatc cttgtatagt attggtaaga taattaaaag atagcaattt
    30061 caattaggag gtctcgcttt ggataacgat cgtcaaaaag ctttagatac agtaattaaa
    30121 aatatggaga aatctttcgg taaaggtgcc gtaatgaagt tgggtgacaa tataggtcgc
    30181 cgagtttcaa ctacatcaac tggttcagtt acattagata atgcgctagg tgtaggtggc
    30241 tatcctaaag gacgaattat tgaaatttat ggtcctgaaa gttctggtaa gacaacagta
    30301 gcgcttcacg ctattgctga agtacaaagt aatggcgggg tggcagcatt tatcgatgct
    30361 gaacatgctt tagatccaga atatgctcaa gcattaggcg tagatatcga taatttatat
    30421 ttatcgcaac cggatcatgg tgaacaaggt cttgaaatcg ccgaagcatt tgttagaagt
    30481 ggtgcagttg atattgtagt tgtagactca gttgctgctt taacacctaa agctgaaatt
    30541 gaaggagaaa tgggagacac tcacgttggt ttacaagctc gtttaatgtc acaagcgtta
    30601 cgtaaacttt caggtgctat ttctaaatca aatacaactg ctattttcat caaccaaatt
    30661 cgtgaaaaag ttggtgttat gttcggtaat ccagagacta caccaggtgg acgtgcatta
    30721 aaattctata gttcagtaag actagaagta cgtcgtgcag aacagcttaa acaaggacaa
    30781 gaaattgtag gtaatagaac taaaattaaa gtcgttaaaa ataaagtggc accaccattt
    30841 agagtagctg aagttgatat tatgtatgga caaggtattt ctaaagaggg tgaacttatt
    30901 gatttaggtg ttgaaaacga catcgttgat aaatcaggag catggtattc ttacaatggc
    30961 gaacgaatgg gtcaaggtaa ggaaaatgtt aaaatgtact tgaaagaaaa tccacaaatt
    31021 aaagaagaaa ttgatcgtaa attgagagaa aaattaggta tatctgatgg tgatgttgaa
    31081 gaaacagaag atgcaccaaa gtcattattt gacgaagaat agtacacaaa tttatatcta
    31141 tagttaaact tagcaaatat ccttatagga ttgattgaaa gtgatattca tctcataaag
    31201 ctagaataat atctaacttt atgggataca ctacaaatcg agactataag gttttttatt
    31261 ttatttatta ttacattatc aatagtttta taatcgagct tcaaaacttt agaaaatagt
    31321 agaaatagca ttcaatatag tgcaaaagtg caaattgata acttgacact tatctcctat
    31381 aaaccgtaca attaatttgt atgatttata tataatttca taaagtcata ttgaatttca
    31441 tataaagagc aaaccctaga aaaggaggtg tttgtgtgaa tttattaagc ctcctactca
    31501 ttttgctggg gatcattcta ggagttgttg gagggtatgt tgttgcccga aatttgttgc
    31561 ttcaaaagca atcacaagct agacaaactg ccgaagatat tgtaaatcaa gcacataaag
    31621 aagctgacaa tatcaaaaaa gagaaattac ttgaggcaaa agaagaaaac caaatcctaa
    31681 gagaacaaac tgaagcagaa ctacgagaaa gacgtagcga acttcaaaga caagaaaccc
    31741 gacttcttca aaaagaagaa aacttagagc gcaaatctga tctattagat aaaaaagatg
    31801 agattttaga gcaaaaagaa tcaaaaattg aagaaaaaca acaacaagta gatgcaaaag
    31861 agagtagtgt tcaaacgtta ataatgaagc atgaacaaga attagaacgc atctccggtc
    31921 tcactcaaga agaagctatt aatgagcaac ttcaaagagt agaggaagaa ctgtcacaag
    31981 atattgcagt acttgttaaa gaaaaagaaa aagaagctaa agaaaaagtt gataaaacag
    32041 caaaagaatt attagctaca gcagtacaaa gattagcagc agatcacaca agtgaatcaa
    32101 cggtatcagt agttaactta cctaatgatg agatgaaagg tcgaatcatt ggacgagaag
    32161 gacgaaacat ccgcacactt gaaactttaa ctggcattga tttaattatt gatgacacac
    32221 cagaagcggt tatattatct ggttttgatc caataagaag agaaattgct agaacagcac
    32281 ttgttaactt agtatctgat ggacgtattc atccaggtag aattgaagat atggtcgaaa
    32341 aagctagaaa agaagtagac gatattatta gagaagcagg tgaacaagct acatttgaag
    32401 tgaacgcaca taatatgcat cctgacttag taaaaattgt agggcgttta aactatcgta
    32461 cgagttacgg tcaaaatgta cttaaacatt caattgaagt tgcgcatctt gctagtatgt
    32521 tagctgctga gctaggcgaa gatgagacat tagcgaaacg agctggactt ttacatgatg
    32581 ttggtaaagc aattgatcat gaagtagaag gtagtcatgt tgaaatcggt gtagaattag
    32641 cgaaaaaata tggtgaaaat gaaacagtta ttaatgcaat ccattctcat catggtgatg
    32701 ttgaacctac atctattata tctatccttg ttgctgctgc agatgcattg tctgcggctc
    32761 gtccaggtgc aagaaaagaa acattagaga attatattcg tcgattagaa cgtttagaaa
    32821 cgttatcaga aagttatgat ggtgtagaaa aagcatttgc gattcaggca ggtagagaaa
    32881 tccgagtgat tgtatctcct gaagaaattg atgatttaaa atcttatcga ttggctagag
    32941 atattaaaaa tcagattgaa gatgaattac aatatcctgg tcatatcaag gtgacagttg
    33001 ttcgagagac tagagcagta gaatatgcga aataattttt gtctccctca caaattagtg
    33061 agggagcttt tttaagttgt agtcttaatc tagttagaca gcactttatc ggtaataact
    33121 atattaaaca gtagttattt gaaagtaaga cggaccttat attaaataag aagttattgc
    33181 ttttaataaa aatgttttag gct
//
LOCUS       DALAGD010000021        32582 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.021, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000021 DALAGD010000000
VERSION     DALAGD010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 32582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32582
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.021"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(64..234)
                     /locus_tag="O2701_000762"
     CDS             complement(64..234)
                     /locus_tag="O2701_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292668.1"
                     /translation="MLRLSIIFIAFIINTTITYGYTTEGTWVNLLFKSLSLSMIIVFM
                     FYYIRFVIEKKR"
     gene            complement(296..394)
                     /locus_tag="O2701_000763"
     CDS             complement(296..394)
                     /locus_tag="O2701_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292669.1"
                     /translation="MIKKKHLSGSLVPDRCFLIISEEVERFMENAY"
     gene            complement(413..1312)
                     /gene="sdaAA"
                     /locus_tag="O2701_000764"
     CDS             complement(413..1312)
                     /gene="sdaAA"
                     /locus_tag="O2701_000764"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HCZ4292670.1"
                     /translation="MFDSIRETIDYAVENNMSFADIMVKEEMELSGKSRDEVRAQMKQ
                     NLDVMRDAVIKGTTGDGVESVTGYTGHDAAKLRDYNETHHALSGYEMIDAVKGAIATN
                     EVNAAMGIICATPTAGSSGTIPGALFKLEKTHDLTEEQMIDFLFTSALFGRVVANNAS
                     VAGATGGCQAEVGSASAMAAAAAVAIFGGSPEASGHAMALAISNLLGLVCDPVAGLVE
                     IPCVMRNAIGSGNALISADLALAGIESRIPVDEVIEAMDKVGRNLPASLRETGLGGLA
                     GTPTGEAIKRKIFGTAEDMVKNN"
     gene            complement(1326..2006)
                     /gene="sdaAB"
                     /locus_tag="O2701_000765"
     CDS             complement(1326..2006)
                     /gene="sdaAB"
                     /locus_tag="O2701_000765"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HCZ4292671.1"
                     /translation="MAKSYDYQSAFDIIGPVMMGPSSSHTAGAVKIGNSARAILGDIP
                     KNIEIHYYESFAQTHQGHGTDVAIVGGAMGYSTFDNRIKSSLEIAADDGIKIDIIEEE
                     GDSIGQHPNCAYIKSSRNDGRYIEIIGISIGGGTIKIKGIHINGLEVDLNHGLPILVV
                     DGNMTKAQVNHFINDINDMKLDCKDEFIKIDEDKCLVVIPLNKAISESTLNTIKEKYS
                     DVNVSYIN"
     gene            complement(2009..3052)
                     /locus_tag="O2701_000766"
     CDS             complement(2009..3052)
                     /locus_tag="O2701_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCZ4292672.1"
                     /translation="MDILLGVGTLVLVLVIMTLFLKYAPYGKQGLQALSGAACATFLP
                     QAFLSYAIGGVFDIKFLQDIGDLAGSLSGIAVGILTCLNLGVAPVFAVIVGLVLHDFK
                     LLPAFIAAYGVAFLIKWIEKKVPEGLDLIVVILFAPAIAFGLASIITPGVLATLKQIG
                     SAVTAVGDNNPYALAVILGLVIPVTGMTPLSSMVLTSLLGLTGVPMAIGALTCTGSSF
                     VNLILFRKLNIGGPSKAFAVCIEPLTQIDLIAQYPVQLYGTNALIGVVNACIVTFSGL
                     VIGVKGMATPIAGAIVLFGFNNAVTSIVTIATVIIVSIVLAYIIGTLINKFNLMNINF
                     KMPSKKNHIKESV"
     gene            3603..3806
                     /locus_tag="O2701_000767"
     CDS             3603..3806
                     /locus_tag="O2701_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292673.1"
                     /translation="MQLSHSVKVAISIYLALIFITFTSYLVIILYTSMTGHDVSHFVL
                     DSQHSHHGSLTQKHLSLPEISFK"
     gene            3989..4924
                     /locus_tag="O2701_000768"
     CDS             3989..4924
                     /locus_tag="O2701_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HCZ4292674.1"
                     /translation="MRYLANNHSALRLKGIILAIVGACLWGLGGTVSDFLFKYKNINV
                     DWYVTARLVVSGVFLLIMYKMMQPKRSIFSVFQDRRMLGKLLIFSILGMLVVQYAYMA
                     SINTGNAAIATLLQYIAPVYIIIWFVIRGVAKLTLFDVLAIIMTLLGTFLLLTNGSFS
                     NLVVNPASLFWGILAGVALAFYTIYPSDLLNRFGSILIVGWAMLISGVAMNLRHPIWH
                     IDITKWDISIILFLIFGIIGGTALAFYFFIDSLQYISAKETTLFGTVEPVVAVIASSL
                     WLHVAFKPFQIVGIILIMILILLLSLKRQPETLDE"
     gene            complement(5006..5974)
                     /locus_tag="O2701_000769"
     CDS             complement(5006..5974)
                     /locus_tag="O2701_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCZ4292675.1"
                     /translation="MRKKWSTLAFGFLVAAYAHIRIKEKRSVKSYMLEQGIRLSRAKR
                     RFMYKEEAMKALEKMAPQTAGEYEGTNYQFKMPVKVDKHFGSTVYTVNDKQDKHQRVV
                     LYAHGGAWFQDPLKIHFEFIDELAETLNAKVIMPVYPKIPHQDYQATYVLFEKLYHDL
                     LNQVADSKQIVVMGDSAGGQIALSFAQLLKEKHIVQPGHIVLISPVLDATMQHPEIPD
                     YLKKDPMVGVDGSVFLAEQWAGDTPLDNYKVSPINGDLDGLGRITLTVGTKEVLYPDA
                     LNLSQLLSAKGIEHDFIPGYYQFHIYPVFPIPERRRFLYQVKNIIN"
     gene            complement(6107..6451)
                     /locus_tag="O2701_000770"
     CDS             complement(6107..6451)
                     /locus_tag="O2701_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCZ4292676.1"
                     /translation="MVKQLNSVEAFREFIHQYPLAVVHVMRDQCSVCHAVLPQIEDLM
                     QSYPNVPLAVINQSQVEAIAGELNIFTVPVDLIFMNGKEMHRQGRFIDMQRFEHHLKQ
                     MNDSVNNDVDEH"
     gene            complement(6493..6903)
                     /locus_tag="O2701_000771"
     CDS             complement(6493..6903)
                     /locus_tag="O2701_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCZ4292677.1"
                     /translation="MTTEGLLVAEKEIEVNGYDIDAMGVVSNIVYIRWFEDLRTAFIN
                     QHMNYSTMINQGISPILMKTEAEYKVPVTIHDKPVGRIYLVKASKMKWVFQFEIVSAH
                     GVHCIGTQTGGFYRLSDKKITSVPQVFQDILATK"
     gene            complement(7283..9349)
                     /locus_tag="O2701_000772"
     CDS             complement(7283..9349)
                     /locus_tag="O2701_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HCZ4292678.1"
                     /translation="MFKKLFGQLQRIGKALMLPVAILPAAGILLAFGNAMHNEQLVEI
                     APWLKNDIIVMISSVMEAAGQVVFDNLPLLFAVGTALGLAGGDGVAALAALVGYLIMN
                     ATMGKVLHITIDDIFSYAKGAKELSQAAKEPAHALVLGIPTLQTGVFGGIIMGALAAW
                     CYNKFYNITLPPFLGFFAGKRFVPIVTSVVAIATGVLLSFAWPPIQDGLNSLSNFLLN
                     KNLTLTTFIFGIIERSLIPFGLHHIFYSPFWFEFGSYTNHAGELVRGDQRIWMAQLKD
                     GVPFTAGAFTTGKYPFMMFGLPAAAFAIYKNARPERKKVVGGLMLSAGLTAFLTGITE
                     PLEFSFLFVAPVLYGIHVLLAGTSFLVMHLLGVKIGMTFSGGFIDYILYGLLNWDRSH
                     ALLVIPVGIVYAIVYYFLFDFAIRKFKLKTPGREDEETEIRNSSVAKLPFDVLDAMGG
                     KENIKHLDACITRLRVEVVDKSKVDVAGIKALGASGVLEVGNNMQAIFGPKSDQIKHD
                     MAKIMSGEITKPSETTVTEEMSDEPVHVEALGTTDIYAPGIGQIIPLSEVPDQVFAGK
                     MMGDGVGFIPEKGEIVAPFDGTVKTIFPTKHAIGLESESGVEVLIHIGIDTVKLNGEG
                     FESLINVDEKVTQGQPLMKVNLAYLKAHAPSIVTPMIITNLENKELVIEDVQDADPGK
                     LIMTVK"
     gene            complement(9650..11389)
                     /locus_tag="O2701_000773"
     CDS             complement(9650..11389)
                     /locus_tag="O2701_000773"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HCZ4292679.1"
                     /translation="MAKIKANEALVKALQAWDIDHLYGIPGDSIDAVVDSLRTVRDQF
                     KFYHVRHEEVASLAAAGYTKLTGKIGVALSIGGPGLIHLLNGMYDAKMDNVPQLILSG
                     QTNSTALGTKAFQETNLQKLCEDVAVYNHQIEKGDNVFEIVNEAIRTAYEQKGVAVVI
                     CPNDLLTEKIKDTTNKPVDTSRPTVVSPKYKDIKKAVKLINKSKKPVMLIGVGAKHAK
                     DELREFIEMAKIPVIHSLPAKTILPDDHPYSIGNLGKIGTKTSYQTMQEADLLIMVGT
                     NYPYVDYLPKKNIKAIQIDTNPKNIGHRFNINVGIVGDSKIALHQLTENIKHVAERPF
                     LNKTLERKAVWDKWMEQDKNNNSKPLRPERLMASINKFIKDDAVISADVGTATVWSTR
                     YLNLGVNNKFIISSWLGTMGCGLPGAIASKIAYPNRQAIAIAGDGAFQMVMQDFATAV
                     QYDLPLTVFVLNNKQLAFIKYEQQAAGELEYAVDFSDMDHAKFAEAAGGKGYTIKSAS
                     EVDAIVEEALAQDVPTIVDVYVDPNAAPLPGKIVNEEALGYGKWAFRSITEDKHLDLD
                     QIPPISVAAKRFL"
     gene            complement(11436..12125)
                     /locus_tag="O2701_000774"
     CDS             complement(11436..12125)
                     /locus_tag="O2701_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HCZ4292680.1"
                     /translation="MNDYVQALLMILLTVVLYYFAKRLQQKYPNPFLNPALIASLGII
                     FVLLIFGISYNGYMKGGSWINHILNATVVCLAYPLYKNREKIKDNVSIIFASVLTGVM
                     LNFMLVFLTLKAFGYSKDVIVTLLPRSITAAVGIEVSHELGGTDTMTVLFIITTGLIG
                     SILGSMLLRFGRFESSIAKGLTYGNASHAFGTAKALEMDIESGAFSSIGMILTAVISS
                     VLIPVLILLFY"
     gene            complement(12118..12513)
                     /gene="cidA"
                     /locus_tag="O2701_000775"
     CDS             complement(12118..12513)
                     /gene="cidA"
                     /locus_tag="O2701_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like murein hydrolase modulator CidA"
                     /protein_id="HCZ4292681.1"
                     /translation="MHKVQLIIKLLLQLGIIIVITYIGTEIQKIFHLPLAGSIVGLFL
                     FYLLLQFKIVPLTWVEDGANFLLKTMVFFFIPSVVGIMDVASEITLNYILFFAVIIIG
                     TCIVALSSGYIAEKMSVKHKHRKGVDAYE"
     gene            complement(12726..13604)
                     /locus_tag="O2701_000776"
     CDS             complement(12726..13604)
                     /locus_tag="O2701_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCZ4292682.1"
                     /translation="MDIKHMKYFIEVVKQGGMTNASKSLYIAQPTISKAIKDIENEMG
                     TPLFDRSKRHLILTDAGQIFYEKSKEIVALYDYLPSEMERLNGLETGHINMGMSAVMN
                     MKILINILGAFHQQYPNVTYNLIENGGKTIEQQIINDEVDIGVTTLPVDHHIFDYTTL
                     DKEDLRLIVSREHRLAKYETVKLEDLAGEDFILFNKDFYLNDKIIENAKNVGFVPNTV
                     AQISQWHVIEDLVTNELGISILPTSISEQLNGDVKLLRIEDAHVHWELGVVWKKDKQL
                     SHATTKWIEFLKDRLG"
     gene            13771..13989
                     /locus_tag="O2701_000777"
     CDS             13771..13989
                     /locus_tag="O2701_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HCZ4292683.1"
                     /translation="MSFYEFMQSFLGDDTPLGELVDWINQDSNFPREVKSHSEILSYF
                     RTNPCPESISVPVIKRALSVFNQFTNVQ"
     gene            14378..14809
                     /locus_tag="O2701_000778"
     CDS             14378..14809
                     /locus_tag="O2701_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCZ4292684.1"
                     /translation="MEYKKILIRLLIAFAVLFSADFTYQSVEQTHQSHAAVNYYSKNQ
                     CTWWAFKRRAQVGKPVSNRWGNAKNWYYNARKSKYATGRTPRKFAVMQSTAGYYGHVA
                     VVEQVYKNGSIKVSEYNFYRPLKYNTRVLSKKAARNFNYIY"
     gene            complement(14947..16224)
                     /locus_tag="O2701_000779"
     CDS             complement(14947..16224)
                     /locus_tag="O2701_000779"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HCZ4292685.1"
                     /translation="MQNLDKNFRHLSRKEKLQQLVDKQWLSEEQFDILLNHPLIDEEV
                     ANSLIENVIAQGALPVGLLPNIIVDDKAYVVPMMVEEPSVVAAASYGAKLVNQTGGFK
                     TVSSERIMIGQIVFDGVDDTEKLSADIKALEKQIHKIADEAYPSIKARGGGYQRIAID
                     TFPEQQLLSLKVFVDTKDAMGANMLNTILEAITAFLKNEFPQSDILMSILSNHATASV
                     VKVQGEIDVKDLARGERTGEEVAKRMERASVLAQVDIHRAATHNKGVMNGIHAVVLAT
                     GNDTRGAEASAHAYASRDGQYRGIATWRYDQDRQRLIGTIEVPMTLAIVGGGTKVLPI
                     AKASLELLNVESAQELGHVVAAVGLAQNFAACRALVSEGIQQGHMSLQYKSLAIVVGA
                     KGDEIAKVAEALKKEPRANTQAAEHILQEIRQQ"
     gene            16478..17644
                     /locus_tag="O2701_000780"
     CDS             16478..17644
                     /locus_tag="O2701_000780"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HCZ4292686.1"
                     /translation="MTIGIDKINFYVPKYYVDMAKLAEARQVDPNKFLIGIGQTEMAV
                     SPVNQDIVSMGANAAKDIITDEDKKKIGMVIVATESAVDAAKAAAVQIHNLLGIQPFA
                     RCFEMKEACYAATPAIQLAKDYLATRPNEKVLVIATDTARYGLNSGGEPTQGAGAVAM
                     VIAHNPSILALNEDAVAYTEDVYDFWRPTGHKYPLVDGALSKDAYIRSFQQSWNEYAK
                     RQGKSLADFASLCFHVPFTKMGKKALESIIDNADETTQERLRSGYEDAVDYNRYVGNI
                     YTGSLYLSLISLLENRDLQAGETIGLFSYGSGSVGEFYSATLVEGYKDHLDQAAHKAL
                     LNNRTEVSVDAYETFFKRFDDVEFDEEQDAVHEDRHIFYLSNIENNVREYHRPE"
     gene            complement(17814..18335)
                     /locus_tag="O2701_000781"
     CDS             complement(17814..18335)
                     /locus_tag="O2701_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCZ4292687.1"
                     /translation="MEYKSYYDSPVGRLELLSDGVSLTAVLFENQQGDGTREENTSLA
                     IFREATQWLDAYFKGDNPEITIPLKPTGSHFQQCVWNELRQVPYGTLTTYGAIAKKVG
                     KLLNKPKMSAQAVGGAVGSNPLSIIVPCHRVVGKTGSLTGFGGTINNKIKLLELENID
                     MSKLYVPKHSTKP"
     gene            18744..20849
                     /gene="clpL"
                     /locus_tag="O2701_000782"
     CDS             18744..20849
                     /gene="clpL"
                     /locus_tag="O2701_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpL"
                     /protein_id="HCZ4292688.1"
                     /translation="MNNGFFNSDFDSIFRRMMKDMQGSNQVGNKKYYINGKEVSPEEL
                     AQLTQQGGNHSAEQSAQAFQQAAQRQQGQQGGNGNYLEQIGRNLTQEARDGLLDPVIG
                     RDKEIQETAEVLSRRTKNNPILVGEAGVGKTAIVEGLAQAIVEGNVPAAIKDKEIISV
                     DISSLEAGTQYRGAFEENIQKLIEGVKSSQNAVLFFDEIHQIIGSGATGSDSGSKGLS
                     DILKPALSRGEISIIGATTQDEYRNNILKDAALTRRFNEVLVNEPSAKDTVEILKGIR
                     EKFEEHHQVKLPDDVLKACVDLSIQYIPQRLLPDKAIDVLDITAAHLSAQSPAVDKVE
                     TEKRISELENDKRKAVSAEEYKKADDIQNEIKSLQDKLENSNGEHTAVATVHDISDTI
                     QRLTGIPVSQMDDNDIERLKNISNRLRSKIIGQDQAVEMVSRAIRRNRAGFDDGNRPI
                     GSFLFVGPTGVGKTELAKQLAIDLFGNKDALIRLDMSEYSDTTAVSKMIGTTAGYVGY
                     DDNSNTLTEKVRRNPYSVILFDEIEKANPQILTLLLQVMDDGNLTDGQGNVINFKNTI
                     IICTSNAGFGNGNDAEEKDIMHEMKKFFRPEFLNRFNGIVEFLHLDKDALQDIVNLLL
                     DDVQVTLDKKGITMDVSQDAKDWLIEEGYDEELGARPLRRIVEQQVRDKITDYYLDHT
                     DVKHVDIDVEDNELVVKGK"
     gene            complement(20918..21088)
                     /locus_tag="O2701_000783"
     CDS             complement(20918..21088)
                     /locus_tag="O2701_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HCZ4292689.1"
                     /translation="MSVIINILIFLAIFGYALYTLVKFFKRSKQGKCGTCDINRDCCG
                     TEQHTANHFPGK"
     gene            complement(21101..23095)
                     /gene="feoB"
                     /locus_tag="O2701_000784"
     CDS             complement(21101..23095)
                     /gene="feoB"
                     /locus_tag="O2701_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HCZ4292690.1"
                     /translation="MENYCILGNPNVGKTSLFNALTGSYEYIGNWSGVTVEKKVGKLK
                     ENVGQLIDLPGTYDLSPISKDETVVTDYLLNDSFSGIINIVDASQLKRNMQLTVQLLE
                     LNQPIYIGLNMIDVATKRGIKIDYHKLMKKLKTPIFPVVARTGKGTKYLLGEIKHLGE
                     GYQPHFKINYGEKIEETIKNMCQIIMTETSHDKYQARFIAIQFLLNNMQIANELNSEV
                     VNKLSSLRDQVAEQVGAVSVRREMERIRNHYIETLLQDVVTYPDEDKQYFSSRIDKIL
                     THKYIGMPIFLAIMWLIFQTTFTWIGTPLSDQLDAFIGGTFTDSVKTIMNYLGVIPFL
                     QDLITDGIIAGVGSVLVFVPQIVVLFFFISLLEDSGYMARIAVLMDRIMESFGLSGKS
                     FIPMIIGFGCNVPSIMAARSIENEKERLTTILIAPFMSCSARLPVYALFVGIFFKENQ
                     SLVVLSLYVLGIIMAFLVSTVLTKTILKNDNAIFIVELPTYRVPSIKTLWRSTWEKAM
                     GFVRKAGTFIFGGSVVIWLLSYVGPHGINVNINQSFLHMVGSFFGMLVQPLGFGTWQA
                     GATLVPGFLAKEVIVSSMAIIYSSGDAGLVNVIQNQFTPLSAYAFMIFILLYIPCVST
                     VAAIRKETYSWKWTALAVAYPLVTAYVLTFIFYQVGHLFV"
     gene            complement(23107..23334)
                     /locus_tag="O2701_000785"
     CDS             complement(23107..23334)
                     /locus_tag="O2701_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HCZ4292691.1"
                     /translation="MLNIKNGEMNKAYKIKRMDIANENMLYRLSAFGLTDDAIITIKQ
                     KCLFKGPCIIEVNGQQLSIRHCDACSIALEE"
     gene            complement(23550..26039)
                     /gene="farE"
                     /locus_tag="O2701_000786"
     CDS             complement(23550..26039)
                     /gene="farE"
                     /locus_tag="O2701_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux MMPL transporter FarE"
                     /protein_id="HCZ4292692.1"
                     /translation="MAKFLYKMGTFIAKHKWSAVIAWIVIVAAILIPLATNAPKFDND
                     IKMTGLESLDTNKKIEKHFNQDSEKAQIRVVFKTTKDDGIVQPNITKDIKKTLDDIKK
                     DDKHIDKISDPYENKQISKDKTTAFADITYDVSQTSLKDGSRDNVKSHLKDLRDNHNV
                     QTELTGTGMTSTEVGGNSELVGIIVAFVVLLITFGSVIAAGLPIISALIGLASGVGII
                     SLLTYAFDIPNVTLTLAVMIGLAVGIDYALFILFRYRQVMKTETDYIKGIGLAIGTAG
                     SAVVFAGVTVVIAVCGLSLVGIDFLAVMGFASAISVVFAVLSALTLLPALISIFHKRI
                     KVNKLQSKFKKDIDTPWSKFITGNALAAVLLGLIILVAAAIPVSHMRLGIPDDGVKPA
                     DSTQKKAYDIISDKFGEGFNGQIPMLINVKDKKDDPQGLQQDLQSVYKDIKDKKNVDI
                     VTPPQMSKDNDYALMVVIPKQGPNAESTNDLVHDLRDYHKDAQDKYGFKTEISGQSVI
                     NIDMSKKLNEAIPLFASVIVVLAFFLLMIVFRSILIPLKAVLGFVLSLMATLGFTTLV
                     MQDGFMKGLFGIETTGPMLAFLPVITIGILFGLAMDYEVFLMSRIHEEYSKTGDNDYS
                     IKVGLKESGPVIVAAALIMFSVFFAFVFQEDVMIKSMGMALAFGVLFDAFVVRMMLIP
                     ALTKLFGKGSWYLPAWLNRIIPRVDIEGHALEKYKTVESQESEAKDSKETYDTTFKVY
                     PQGATNVSKHQDAHGQDDAHSIVLDDKTMALYQEVKQQSASSLFLYDALIDYQNKHQL
                     NSKKQATNIEQLNKNIEKLNQLLEKNLRNKS"
     gene            26184..26732
                     /gene="farR"
                     /locus_tag="O2701_000787"
     CDS             26184..26732
                     /gene="farR"
                     /locus_tag="O2701_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux pump transcriptional regulator
                     FarR"
                     /protein_id="HCZ4292693.1"
                     /translation="MKETDLRVIKTKKALSSSLLQLLEQQLFQTITVNQICDNALVHR
                     TTFYKHFYDKYDLLEYLFNQLTKDYFARDISDRLNHPFQTMSDTINNKEDLREIAEFQ
                     EEDAEFNKVLKNVCIKIMHNDIKNNRDRIDIDSDIPDNLIFYIYDSLIEGFIHWIKDE
                     KIDWPGEDIDNIFHRLINIKIK"
     gene            complement(26809..26976)
                     /locus_tag="O2701_000788"
     CDS             complement(26809..26976)
                     /locus_tag="O2701_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292694.1"
                     /translation="MRYVISIIMGIVLAIWSFKQLSQSHLDSGFIFFFIVYVLCISCF
                     NSDKHDKNKKR"
     gene            complement(27088..28632)
                     /gene="pruA"
                     /locus_tag="O2701_000789"
     CDS             complement(27088..28632)
                     /gene="pruA"
                     /locus_tag="O2701_000789"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HCZ4292695.1"
                     /translation="MVVEFKNEPGYDFSVQENVDMFKKALKDVEKELGQDIPLVINGE
                     KIFKDDKIKSINPADTSQVIANASKATKQDVEDAFKAANEAYKSWKTWSANDRAELML
                     RVSAIIRRRKAEIAAIMVYEAGKPWDEAVGDAAEGIDFIEYYARSMMDLAQGKPVLDR
                     EGEHNKYFYKSIGTGVTIPPWNFPFAIMAGTTLAPVVAGNTVLLKPAEDTPYIAYKLM
                     EILEEAGLPKGVVNFVPGDPKEIGDYLVDHKDTHFVTFTGSRATGTRIYERSAVVQEG
                     QNFLKRVIAEMGGKDAIVVDENIDTDMAAEAIVTSAFGFSGQKCSACSRAIVHKDVYD
                     EVLEKSIKLTKELTLGNTVDNTYMGPVINKKQFDKIKNYIEIGKEEGKLEQGGGTDDS
                     KGYFVEPTIISGLKSKDRIMQEEIFGPVVGFVKVNDFDEAIEVANDTDYGLTGAVITN
                     NREHWIKAVNEFDVGNLYLNRGCTSAVVGYHPFGGFKMSGTDAKTGSPDYLLHFLEQK
                     VVSEMF"
     gene            complement(28822..29421)
                     /locus_tag="O2701_000790"
     CDS             complement(28822..29421)
                     /locus_tag="O2701_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCZ4292696.1"
                     /translation="MTEKEKMLAEKWYDANFDQYLINERARAKDICFELNHTRPSATN
                     KRKELIDQLFQTTTDNVSISIPFDTDYGWNVKLGKNVYVNTNCYFMDGGQITIGDNVF
                     IGPNCGFYTATHPLNFHHRNEGFEKAGPIHIGSNTWFGGHVAVLPGVTIGEGSVIGAG
                     SVVTKDIPPHSLAVGNPCKVVRKIDNDLPSETLNDETIK"
     gene            29660..29851
                     /gene="cwrA"
                     /locus_tag="O2701_000791"
     CDS             29660..29851
                     /gene="cwrA"
                     /locus_tag="O2701_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall inhibition responsive protein CwrA"
                     /protein_id="HCZ4292697.1"
                     /translation="MRILITSTVAILIILGLVKTIQDYEMTNDTSRQLSDNKDDDKVI
                     HLNNFKNLHAKEFNPSDFF"
     gene            30091..32499
                     /locus_tag="O2701_000792"
     CDS             30091..32499
                     /locus_tag="O2701_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HCZ4292698.1"
                     /translation="MANTKKTTLDITGMTCAACSNRIEKKLNKLDDVNAQVNLTTEKA
                     TVEYNPDQHDVQEFINTIQHLGYGVAVETVELDITGMTCAACSSRIEKVLNKMDGVQN
                     ATVNLTTEQAKVDYYPEETDADKLVTRIQKLGYDASIKDNNKDQTSRKAEALQHKLIK
                     LIISAVLSLPLLMLMFVHLFNMHIPALFTNPWFQFILATPVQFIIGWQFYVGAYKNLR
                     NGGANMDVLVAVGTSAAYFYSIYEMVRWLNGSTTQPHLYFETSAVLITLILFGKYLEA
                     RAKSQTTNALGELLSLQAKEARILKDGNEVMIPLNEVHVGDTLIVKPGEKIPVDGKII
                     KGMTAIDESMLTGESIPVEKNVDDTVIGSTMNKNGTITMTATKVGGDTALANIIKVVE
                     EAQSSKAPIQRLADIISGYFVPIVVGIALLTFIVWITLVTPGTFEPALVASISVLVIA
                     CPCALGLATPTSIMVGTGRAAENGILFKGGEFVERTHQIDTIVLDKTGTITNGRPVVT
                     DYHGDNQTLQLLATAEKDSEHPLAEAIVNYAKEKQLILTETTTFKAVPGHGIEATIDH
                     HYILVGNRKLMADNDISLPKHISDDLTHYERDGKTAMLIAVNYSLTGIIAVADTVKDH
                     AKDAIKQLHDMGIEVAMLTGDNKNTAQAIAKQVGIDTVIADILPEEKAAQIAKLQQQG
                     KKVAMVGDGVNDAPALVKADIGIAIGTGTEVAIEAADITILGGDLMLIPKAIYASKAT
                     IRNIRQNLFWAFGYNIAGIPIAALGLLAPWVAGAAMALSSVSVVTNALRLKKMRLEPR
                     RKDA"
BASE COUNT        10399 a         6175 c         5056 g        10952 t
ORIGIN      
        1 ttacattgat ttacattgtc ccttttattt attcttttca aatactatcc ccatagcttt
       61 gatttaacgc tttttctcaa taacaaaacg aatatagtag aacatgaaaa cgataatcat
      121 gctgagcgat aaagatttaa ataatagatt gacccacgtt ccctcagtcg tatatccata
      181 tgtaatcgtt gtgttaatga tgaatgctat aaagatgatt gatagtctta gcatatcatc
      241 actcctttta agttatttta gatatacggg ggcgcttttg caatcactat tttgattagt
      301 atgcattttc cataaatctt tcaacttctt cagagataat taagaagcat ctatctggta
      361 ctaatgatcc agacagatgc ttctttttta tcaatatttt attgttatct cattaattat
      421 ttttaaccat atcttcagct gtgccaaaga ttttacgttt aattgcttcg ccagttggtg
      481 tgcctgctag tccacccaat ccagtttcac gtaatgatgc aggaaggtta cgaccaacct
      541 tatccattgc ttcaataact tcatcaacag ggattctact ttcaatacct gctaatgcta
      601 aatctgctga aattaaagcg ttacccgaac caattgcatt tctcataaca caaggaattt
      661 caacaagtcc ggctactgga tcacaaacta aacctaataa attacttatc gctaatgcca
      721 tagcgtgccc ggatgcttct ggtgatcctc cgaatatagc tactgctgca gctgcggcca
      781 ttgcagatgc tgaaccaact tcagcttggc agccacctgt tgcaccagct acacttgcat
      841 tgtttgctac gacacgccca aacaatgctg aagtgaataa gaaatcaatc atttgctctt
      901 ctgttaaatc atgtgttttt tctaatttaa aaagtgcacc gggaatggta cccgaggaac
      961 cagctgttgg cgttgcacaa ataataccca tcgcagcatt gacttcattt gttgcaatgg
     1021 cacctttgac tgcgtcaatc atttcatatc cagacaaagc atgatgtgtt tcattataat
     1081 cacgtagttt agcagcatca tgaccagtgt agcccgttac actttcaacc ccatcacctg
     1141 tcgtcccttt gattactgcg tctcgcatga catctaaatt ttgtttcatt tgcgctcgca
     1201 cttcatcacg tgatttaccg cttaattcca tttcttcttt aaccatgata tccgcaaatg
     1261 acatattatt ttctacggca taatctatag tctctctaat tgaatcaaac atgtttattc
     1321 cccctctaat ttatatagga aacgtttacg tcactgtatt tctctttaat tgtatttaat
     1381 gttgattctg agattgcttt atttaatggt attacaacca agcatttatc ttcatctatc
     1441 ttaataaatt catctttaca gtctaatttc atatcgttga tatcattgat gaaatgattt
     1501 acttgtgctt tagtcatatt tccgtcaaca actaaaattg gtaatccatg atttaaatct
     1561 acttctagtc catttatatg aataccttta attttaattg taccaccacc gattgaaata
     1621 ccgataattt caatgtagcg accatcatta cgagatgatt tgatataagc acagtttgga
     1681 tgttgaccaa tactatcgcc ttcttcttcg atgatatcta ttttaatacc atcatcagct
     1741 gcaatttcta atgaagattt aattcggtta tcaaatgttg aatatcccat tgctccaccc
     1801 acaatagcga catctgtacc atgtccttgg tgtgtttgag caaatgattc ataataatgt
     1861 atttcaatat ttttaggaat atctcccaat attgcgcgtg ctgaattccc aatctttact
     1921 gcaccagccg tatgagaact tgaagggccc atcataactg gtccgataat atcgaaagca
     1981 ctttgataat catagctctt tgccataatt aaacactctc cttaatatga ttctttttgc
     2041 tcggcatttt aaagttgata ttcattaaat taaacttatt aattagtgtt ccaataatat
     2101 aggctaagac aatgctgaca ataatcactg ttgcgattgt tacaattgat gtcactgcat
     2161 tattaaagcc aaacaatacg atggcgcctg caattggtgt tgccatacct ttgacaccta
     2221 ttactagtcc gctaaatgtc acgatacatg cgttgacgac cccaatcagt gcatttgtac
     2281 catatagttg tactggatat tgcgctatta aatcaatttg cgtcaatggc tcaatacaaa
     2341 ctgcaaatgc ttttgacggt ccaccaatgt ttaattttcg gaataaaata aggttaacaa
     2401 atgagctacc tgtacatgtt agtgctccaa tagccatagg aacacctgtc agtcctaata
     2461 aacttgttaa taccattgaa cttagcggtg tcatacctgt aacaggaatc actagtccta
     2521 aaatgaccgc taatgcatat ggattgttat cacctaccgc agtgacagca ctacctattt
     2581 gttttaatgt tgctagcaca ccaggtgtaa tgattgatgc aagtccgaaa gcaattgctg
     2641 gtgcaaataa gatcaccaca attaagtcca agccttctgg aactttcttt tcaatccatt
     2701 taattaaaaa agctacgcca taagctgcga tgaatgctgg taataattta aagtcatgta
     2761 atactaaacc aacaatgacc gcaaatactg gtgcaacgcc taagtttaag cacgttagaa
     2821 tacctactgc gataccgctt aaacttcctg ctaaatcccc aatatcttgt agaaatttaa
     2881 tatcaaatac gccaccaata gcataactta agaatgcttg tggtagaaat gtcgcacaag
     2941 ctgcaccgga taatgcttgt agtccttgtt taccgtacgg tgcatacttt aaaaatagcg
     3001 tcatgatcac taaaaccaag actaatgtgc ctacacctaa cagaatatcc atttcccaaa
     3061 aacctctctc tatgtttatt ttattttcag accataaaca tcgtacaccc ttaagaaaac
     3121 gttttcaact tttatctgtt atcaaatcaa atatttaagt gaaatatttg atatttgtga
     3181 aagattttta aaatggattg tttcaaaaac tacttatgtt gtcgttaata tttactaatt
     3241 aactttttac tctatatttc aaacagttgt gtgacagttt tttgataact tttttacatc
     3301 tgaaagtaag taatatttct aaaaactttt aatatttata cactttatct ttcgagctat
     3361 ttattttttg tttttgtgct gtgtttataa aaccgcacac tatattcaaa aataacttat
     3421 tacacatata tttgcaataa gataattaaa gtaggatatt atttttagtt ttctgatagg
     3481 aatgatgata gtctataggt tgaatcttta ctttttttta aagctaaatt tacatcaact
     3541 taacaatggt tggttatact gaagatgaaa atatttagta taacttagtg gaggcgataa
     3601 aggtgcaatt gagtcattcc gttaaagttg caatttctat ctatttagca cttatcttta
     3661 taacgttcac ttcttattta gtcattattt tatatacgag tatgactgga catgatgtat
     3721 cacatttcgt gttagatagt cagcattctc atcatggatc tcttacgcaa aaacatttga
     3781 gtcttcctga aatctcattt aaatagttta tcctctgtgt ttcaacattc atttcccata
     3841 tcgattcatt tatctatcat ctagaccact acatcttaga tgattttttt attttctcat
     3901 ttcactcttt cttaaaagtc gatataatga attaaatcat tatcatacac cgacatattt
     3961 tatgttgttg gtgttaagtt ttaaaggggt gagatacttg gcgaataatc attcagcttt
     4021 gcgtttaaaa ggaattatac ttgccattgt cggtgcttgt ttatggggat taggtggtac
     4081 tgtttctgat ttcttgttca aatataagaa tattaatgtc gattggtacg tcactgctcg
     4141 acttgtagtc agtggtgttt tcttacttat tatgtacaaa atgatgcaac ccaaacgttc
     4201 aatatttagc gtattccaag atcgacgtat gttaggcaaa ttacttatct tcagtatact
     4261 gggcatgtta gtagtacaat atgcttatat ggcatctatt aatacaggta atgctgcgat
     4321 tgcaacatta ctacaataca ttgcgccagt ttatattatt atttggtttg tcataagagg
     4381 cgttgcaaaa ctaacattat ttgatgtgct tgctattatc atgacactat taggaacatt
     4441 tttattatta acaaatggtt cattttctaa tttagtcgtc aatcctgcaa gtttattctg
     4501 gggtatttta gctggtgtag cactcgcttt ttacacaatt tatccttcag acctacttaa
     4561 ccgcttcggt tcgattctaa ttgtcgggtg ggcaatgctt atttctggtg ttgcgatgaa
     4621 tttacgccat ccaatttggc acattgatat cactaaatgg gacatatcaa ttatattatt
     4681 tttaatcttt ggtattatcg gtggtaccgc actcgcattt tatttcttta tcgacagttt
     4741 acaatacata tcagcgaaag aaacaacatt attcggaact gttgaacctg tcgtagccgt
     4801 tatcgcaagc agtctatggt tacatgtggc attcaaacca tttcaaatcg taggcatcat
     4861 tcttattatg attttaattt tattactatc acttaaaaga caacctgaaa cattagatga
     4921 ataagaaaac tctgataatc actttagcaa gtaactatta tttaacaacg tagttacctt
     4981 ataggtgata tcagagtttt ttattttagt taataatatt tttcacttgg tataaaaagc
     5041 gtcgtcgctc tggtatcgga aatactggat aaatatggaa ttggtaatat ccaggtataa
     5101 agtcatgttc gatacctttc gcactcaaca attgcgataa gttcaaagca tctgggtaca
     5161 gtacttcttt tgtaccaaca gttaatgtaa tacgacctaa accatctaag tcaccattaa
     5221 ttggtgatac tttgtagtta tctaaaggtg tgtcccctgc ccattgttca gctaagaaca
     5281 cactgccatc cacacctacc attgggtctt tctttaagta gtcaggaatt tcaggatgct
     5341 gcatcgttgc atctaaaact ggtgaaatta atacaatatg tcctggttgc acaatatgtt
     5401 tttcttttaa caattgagca aatgataaag caatttgacc gcccgcagag tcacccatta
     5461 caacgatttg tttagaatct gctacttgat tcaataaatc atggtacaac ttttcaaaaa
     5521 gcacatacgt cgcttgataa tcttgatgcg gaatcttcgg atatactggc atgatgactt
     5581 tagcattgag tgtttctgca agttcatcaa taaattcgaa atgaattttg agtgggtctt
     5641 ggaaccatgc gcctccatgt gcatataata caacgcgttg atgcttatct tgtttatcgt
     5701 taacggtata aacggttgaa ccgaagtgct tatccacttt tactggcatc ttaaactgat
     5761 aattggttcc ctcatattcg cctgctgtct gtggcgccat tttttctaat gctttcatcg
     5821 cttcttcttt atacataaaa cgacgcttag ctctagataa tcgtatacct tgttctaaca
     5881 tataactttt cacactgcgt ttttctttaa ttctaatatg tgcgtatgct gcaactaaaa
     5941 atccaaacgc aagtgtagac cattttttac gcatttgtcg tcactcctct ttgatgattt
     6001 ctactatagt tctactgtac catgctgatt ctaattacta ccaatattta ctgggaaata
     6061 agttatgatg tctacataat cttagcaatg ctaatcattt gcgatattaa tgctcatcga
     6121 catcgttatt tacactatca ttcatttgct taagatgatg ttcaaaacgt tgcatatcga
     6181 taaaacgccc ttgacgatgc atttcttttc cattcataaa aattaaatcc acaggtacag
     6241 tgaaaatatt taattctcca gcaatagctt ccacctgact ttgattaatc acagctaatg
     6301 gcacattggg atatgattgc atcaagtctt caatttgtgg taaaacggca tgacacacgc
     6361 tacactgatc gcgcatgaca tgtacaactg ctaacggata ttgatgaata aattcacgga
     6421 atgcttcgac actatttaat tgcttaacca tttcgaacac cttcttactt tttatatttt
     6481 aaaatgaagt cattattttg ttgctaaaat gtcttgaaac acttgtggca cagaggttat
     6541 cttcttatca ctcaatctgt aaaaaccgcc tgtctgtgta ccaatacaat gcacgccatg
     6601 tgcggacaca atttcaaact gaaacaccca tttcatcttg cttgctttaa ctaagtaaat
     6661 acgacctact ggtttgtcat gtattgtgac aggtacttta tactctgctt ccgttttcat
     6721 aagtataggt gaaatgcctt gattgatcat tgttgagtaa ttcatgtgct gattaataaa
     6781 cgctgttctc aaatcttcga accatctaat ataaacgata ttactaacga cacccatcgc
     6841 atcaatgtcg taaccattca cttcgatttc tttctctgca actaatagac cttctgttgt
     6901 cataatgaca aatctcctta tcttacaaat agatttaaaa tcatgttaat ttatttttat
     6961 tactttcatg ataaaaatac aagcaattga actttattac ggaaaatcaa acttttcaat
     7021 gagactatta atttggctag tcgcgaagat gcatttttct aaaatgtgac aaaaacataa
     7081 gcaatgtttt ctatatctaa cactaatatt taacttatta tcatcaacac acctgctata
     7141 catcattgcg cactaaaaag gcagtagctt cattttaagc tactgccttt tgtaacgtat
     7201 tctctttcaa ctcgctactt cgcatacgca ctacgaatga ctaaaagttc attcatttga
     7261 tatgctgttt catttttaat cattatttga ctgtcataat tagcttacct ggatcagcat
     7321 cttgtacatc ttcaatgaca agttctttat tttcaagatt tgtaataatc attggtgtaa
     7381 cgatgcttgg tgcgtgtgct ttcaagtatg ctaaattcac tttcattaat ggttgacctt
     7441 gtgttacttt ttcatcaacg ttaatcagac tttcgaatcc ttcaccattc agtttcactg
     7501 tatcgatacc aatatgaata agtacttcga cgccactttc agattctaat cctatcgcat
     7561 gtttcgtagg gaagattgtt ttcactgtac catcaaacgg tgctacaatt tcaccttttt
     7621 cagggataaa gccaacacca tcacccatca ttttaccagc gaacacttga tcaggtactt
     7681 ctgataatgg aatgatttga ccgatacctg gtgcatagat gtctgttgtt ccaagtgctt
     7741 ctacgtgaac tggttcatct gacatttctt cagtcactgt cgtttcactt ggtttcgtaa
     7801 tttcaccact cataatcttg gccatatcat gtttaatttg atctgatttt ggaccaaaga
     7861 tagcttgcat attgtttcca acttctaata cacctgatgc gcctaaagct ttaatacctg
     7921 ctacatctac ttttgattta tcaaccactt ctacgcgtag acgtgtaata catgcatcta
     7981 aatgtttaat gttttctttt ccacccattg catctaagac atcaaatggt aattttgcga
     8041 cactagagtt acgaatttca gtttcttcat cttcacgacc tggtgttttc aatttaaact
     8101 tacgaattgc aaagtcgaat aagaagtaat acacgatagc atatacaata ccgactggaa
     8161 taactaataa tgcgtgtgaa cgatcccagt ttaataaacc atataaaata taatctatga
     8221 aaccacctga gaatgtcata ccaattttaa cgcctaataa atgcattact aagaatgatg
     8281 taccagctaa taatacgtga attccataaa gtactggtgc tacaaataag aatgaaaatt
     8341 ctaatggctc agtgatacca gttaaaaatg cagttaatcc tgctgataac attaaaccac
     8401 ccacgacttt tttacgttct ggtcgtgcat ttttataaat agcaaatgcc gccgctggta
     8461 aaccaaacat cataaatgga tatttaccag tagtaaatgc accagcagta aatggtacgc
     8521 catctttcaa ttgtgccatc caaatacgtt ggtcaccacg aaccaattca cctgcgtgat
     8581 ttgtataact tccgaattca aaccagaacg gtgaatagaa aatatgatgt aaaccaaatg
     8641 gaattaatga gcgttcaata ataccgaata taaacgttgt taatgttaaa tttttattta
     8701 ataagaaatt cgataaacta tttaatccat cttgaattgg tggccacgca aagcttaaaa
     8761 gcacacctgt tgcgattgct acgaccgatg tcacaatcgg tacaaatcgt ttacctgcaa
     8821 agaatcctaa aaatggtggt agtgtaatat tataaaattt gttgtaacac catgcggcta
     8881 aagcacccat gataatacca ccaaacacac ccgtttgtaa cgttggaata cctaatacta
     8941 aagcatgtgc tggttctttc gctgcttgac ttaattcttt tgcccctttg gcatatgaga
     9001 aaatgtcatc aattgtaatg tgcaacactt tccccattgt tgcattcata attaagtaac
     9061 ctactagcgc tgctaatgct gcaacaccgt ctcctcctgc taatccaagt gctgtaccaa
     9121 ctgcaaataa taatggcaag ttatcaaata caacttgtcc tgctgcttcc atgaccgacg
     9181 aaatcattac aatgatatcg ttttttaacc atggtgcaat ttctactaat tgttcgttgt
     9241 gcattgcgtt accaaacgct aataaaatac cagctgctgg taaaatcgca acaggtaaca
     9301 ttaatgcttt accgatacgt tgcaattgtc caaacaattt cttaaacata taaacatccc
     9361 ctatctcaat ttccgcttcc atgtacaaaa aaagcaggca tgaacaaaca agactattct
     9421 catagtgcgg ttgtttgctc atgcctgcat taccagtaac acgcaagtga tatctaattc
     9481 atagctatta tacatatttg taaaagcgtt ttcaacaact ttttcacaat tttatcatca
     9541 attcagtacg atcatttgtg ataaataaac aaaacggcaa ttcccatttt gttgaaatag
     9601 aaattaccgt ttttatagtt caattcaatt gtgataacct ttaaatcagt tataagaaac
     9661 gttttgctgc cactgaaatt ggtggaattt gatctaaatc taaatgttta tcttcagtaa
     9721 ttgatctaaa tgcccactta ccataaccaa gcgcttcttc atttacaatt ttacctggta
     9781 atggcgcagc attaggatca acatatacat ctacaatcgt tggtacatct tgtgctaatg
     9841 cctcttcgac tatagcatct acttcgctag cactcttaat tgtataacct ttaccacctg
     9901 ctgcctcagc aaattttgca tgatccatat cagaaaaatc aactgcatat tctaattcac
     9961 cagctgcttg ttgttcatat ttaataaatg ctaactgttt gttattaagt acaaatacag
    10021 ttaaaggtaa atcatattgt actgctgtag cgaagtcttg cattaccatt tggaatgcac
    10081 cgtcaccagc aattgcgatg gcttgtctat ttggatatgc aatttttgat gcaattgcac
    10141 ctggaagacc gcaacccatt gtacctaacc aacttgaaat gatgaacttg ttatttacac
    10201 caaggtttaa gtatcgagtt gaccaaactg ttgctgtacc tacatctgct gaaatcactg
    10261 catcatcttt aataaattta ttgattgatg ccattaatcg ttctggacgt aatggtttac
    10321 tattattatt tttatcttgt tccatccatt tatcccaaac cgctttacgt tctaacgttt
    10381 tgtttaagaa tggtctttca gcaacatgtt taatattttc agttaactga tgcaacgcaa
    10441 ttttactatc tccaacaatt cctacattaa tattgaaacg atgtccgata tttttaggat
    10501 ttgtgtcaat ttgaatggct ttaatatttt tcttaggtaa gtaatccaca tatggatagt
    10561 ttgtaccaac cataattaat aaatccgctt cctgcattgt ttgataagat gttttggtac
    10621 cgattttacc taagttaccg atactatatg gatgatcatc cggcaagatt gttttagctg
    10681 gtaatgaatg aatgacagga attttagcca tttcaataaa ttcacgtagc tcatctttcg
    10741 catgtttcgc acctacacca attaacatga caggcttttt acttttatta attagtttaa
    10801 ccgctttttt gatgtcttta tattttggtg atactactgt tggtcttgat gtatctactg
    10861 gtttattcgt tgtatcttta attttttcag ttaataagtc gttaggacaa ataacaacag
    10921 ctacaccttt ttgttcatat gccgtacgaa ttgcttcgtt aacgatttca aacacattgt
    10981 cacctttttc aatttggtga ttataaacgg ctacatcttc acataatttt tgtaaatttg
    11041 tttcttggaa tgctttcgtt ccaagtgctg tactattcgt ttgtccagat aatattaatt
    11101 gcggtacatt atccattttg gcatcataca taccatttaa taaatgaatt aaaccagggc
    11161 caccgatact taatgccaca ccgattttac cagttaattt tgtgtaacca gcagccgcta
    11221 agcttgctac ttcttcatga cgtacatgat aaaatttaaa ttgatctctc actgtacgta
    11281 aactatcgac tactgcgtcg attgagtctc ctggaatacc atacaagtga tctatatccc
    11341 atgcttgtaa tgctttaact aatgcttcat ttgcttttat ttttgccatt attactaata
    11401 gcctcccttt ctgtctatca ttttaaatat ctaaattaat agaataataa aattagaaca
    11461 ggtattaaca ctgaactaat aactgcagtt aaaatcatcc caattgaact aaatgcaccg
    11521 gattcaatat ccatttctag tgctttagct gtgccaaatg catgtgacgc attcccatac
    11581 gttaatcctt tggcgataga agattcaaat cttccaaatc ttaataacat cgaacctaaa
    11641 atactaccga ttaaacccgt tgtgataata aaaagtacgg tcatcgtatc tgtaccacct
    11701 agttcatgtg acacttcgat acctactgcg gctgttatag atcggggcaa taacgttaca
    11761 atgacgtctt tagaatagcc aaatgcttta agtgttaaga acactaacat gaaattcagc
    11821 atgacgccag ttaatacact tgcaaaaatg atagagacat tgtctttaat tttctctcta
    11881 tttttataaa gtgggtacgc taaacatacg accgttgcgt ttaaaatatg gttgatccaa
    11941 ctgccacctt tcatataccc gttataacta attccaaaga taagtaagac aaaaataatt
    12001 cctaaagatg caattaatgc tggattcaaa aatgggttcg gatatttttg ttgtaacctt
    12061 ttagcgaaat aatataaaac gacagtcaac aaaatcatta ataaggcttg cacgtaatca
    12121 ttcataagcg tctacacctt tacgatgttt atgtttaaca gacatttttt cagcaatata
    12181 acctgaagat aatgcaacga tacatgttcc tatgataatg actgcgaaaa agagtatata
    12241 atttagcgta atttcggaag caacatccat aatacctaca actgacggta tgaagaaaaa
    12301 gaccatcgtc tttaataaaa agtttgcacc gtcttctacc caagttagcg gtacaatctt
    12361 aaattgtaat agtaaataaa ataaaaatag accaacaata ctgccggcta agggaagatg
    12421 aaaaatcttt tgaatttctg tgccaatata agtaatcaca atgatgattc ctagttgtag
    12481 tagtagtttg attattaatt ggactttgtg catggcgcca tccctttcta aatatgtcta
    12541 aattgttaca ataactatta taaagatggc gtacacaata taaaaatact ctttttttat
    12601 agtttgtatt actattttct atggaaacgc tctctaaatt tcacttcatt ttcacaaaaa
    12661 aacactcatt atttcaatga cccacgtaat gatgcgcctt aaataatgag tgctttatta
    12721 atatgttagc ctaaacggtc tttcaaaaat tctatccatt tcgttgtggc atgacttaat
    12781 tgtttatcct tcttccaaac aacacctaat tcccaatgta catgagcatc ttcaatgcgt
    12841 agcagcttca catctccatt tagttgctct gaaattgatg ttggtaaaat actaatacct
    12901 aattcattcg taactaaatc ttctataaca tgccattgtg aaatttgcgc tacagtattc
    12961 ggaacaaagc caacgttttt agcattttca ataattttat cattcaagta aaagtcttta
    13021 ttaaataaaa tgaagtcttc acctgctaaa tcttcgagtt taacagtttc atattttgcg
    13081 agtcgatgct ctctgctcac gataagtcgc aaatcttcct tatctagggt agtataatcg
    13141 aaaatatgat gatcgactgg caaagtggtc acgcctatat ctacttcatc attgataatt
    13201 tgctgttcaa ttgttttacc gccattttct atcaaattat atgtaacatt tggatattgt
    13261 tgatggaatg caccaagaat attgataaga atcttcatat tcatgactgc cgacatgccc
    13321 atgtttatat gtcctgtttc cagtccattc aagcgttcca tttcagatgg taaataatca
    13381 tacagtgcaa caatttcttt acttttctca taaaaaattt gacctgcatc ggtaagaatt
    13441 aaatgtcttt tacttctatc aaataagggc gtgcccattt cattttcaat atctttaatt
    13501 gctttactaa ttgtaggttg tgcaatatat aatgatttgg aagcattagt catgcctcct
    13561 tgcttaacga cttcaataaa atatttcata tgtttgatat ccactaacat catcctaact
    13621 atttcaacac gtttatttca ttatacacaa cttcttaaac attcaataaa tttagattta
    13681 taaaattgct ttataaaaag ttttatgtaa aatacacatt cttttatggt attatattaa
    13741 tggctcaagt atattaatcg aggtgaggta ttgagctttt acgaatttat gcaaagtttt
    13801 cttggtgatg acacaccatt aggtgaattg gttgattgga ttaatcaaga tagcaatttc
    13861 cctagagaag tgaagagcca tagtgaaata ttgtcatatt ttcgaacaaa tccatgtcct
    13921 gaaagcatct cagtacctgt aattaaacgg gcactatcag ttttcaacca attcacaaat
    13981 gtacaatgat acatgaatac atggagtcac atcatctcat gttagcgctc tgattgcagg
    14041 gcgctatttt taatgttatt gctacattta gtatattgtg attgctattt tctttcagtt
    14101 atagaaccat gtaccttttt tttaaatcat tttatatttt cccactaata tccgctgtta
    14161 atcaatcctg acatccttgt atcactatga caattaattg ttaaatacat gaatttctac
    14221 attttatgaa aaaatccatt tttattacaa ttcaacactt tatatgacaa cttcattaca
    14281 gttactttta ttgttgattg cttacattgt tttctaaaaa aaatttgtta tcataattaa
    14341 cgttgaataa agaaaaaaat taacttggga gataaaaatg gaatataaaa agatactaat
    14401 tcgtttatta attgcttttg cagtactttt ctcagcagat ttcacttatc aatccgtcga
    14461 acaaacgcat caatcgcatg ccgcagttaa ttattatagt aaaaaccaat gtacatggtg
    14521 ggcatttaaa cgtcgcgcac aagtcggtaa acctgtttct aatagatggg gcaatgctaa
    14581 aaattggtat tacaatgcac gtaaatcaaa atatgcgact ggtcgtacac caagaaaatt
    14641 tgctgtcatg caatcaactg caggatatta tggacatgtc gcagttgttg aacaagtata
    14701 taaaaacggt agtattaaag tttcagaata caacttttat cgcccattaa aatacaatac
    14761 acgtgtacta agcaaaaagg cagcacgtaa ctttaactat atttactaat caaaaaactt
    14821 ctatcacgaa cgcttcaatt tcctgtatgc gtgtgataga agtttttatt tttgttcata
    14881 ttaatttatc taagcgctac gatggactga cttaaacttt tctttaaaat cgctattcgc
    14941 catttactat tgttgtctaa tttcttgtaa aatatgttcc gctgcttgtg tatttgcacg
    15001 gggttctttt ttcaaagctt cagctacttt agcaatttca tcaccttttg cccctacaac
    15061 gatagctaat gatttatatt gtaaactcat atgaccttgt tgaatacctt ctgacacaag
    15121 cgcgcgacat gctgcaaagt tttgcgctaa accaacggca gcaactacat gacctaattc
    15181 ttgtgctgac tctacattta gtagctctaa tgaagcttta gcaattggta atacttttgt
    15241 accaccgcca acgattgcca atgtcatagg cacttcaatt gtaccaatca atcgttgacg
    15301 atcttgatcg taacgccatg tagcaatacc acgatactgt ccgtcacgac tcgcgtatgc
    15361 atgcgcactt gcttctgcac cacgcgtatc atttcctgtt gctaaaacaa cagcatgtat
    15421 gccattcata acacctttat tatgtgttgc tgcacgatga atatctactt gggccaatac
    15481 agaagcacgt tccattcgtt tggcaacctc ttctccagtt ctctcgcccc ttgctaaatc
    15541 tttaacatca atttcgcctt gaactttaac aacggacgct gttgcatgat tggataaaat
    15601 actcattaaa atgtcgcttt gcggaaattc attttttaaa aatgcagtta tggcctctaa
    15661 aatcgtatta agcatattag cgcccatagc atctttcgta tcaacaaata cttttaaaga
    15721 tagtaactgt tgctcaggaa atgtatcaat cgctatacgt tggtaaccac caccacgcgc
    15781 tttaatagaa ggatatgcct catccgcaat tttatgaatt tgcttttcta aagctttaat
    15841 gtctgctgat aatttttcag tatcgtcaac gccatcaaag acgatttgac ctatcataat
    15901 acgttcagaa gataccgttt taaatccgcc agtctgattc actagctttg caccataact
    15961 agctgcagcg acaactgaag gctcttccac catcataggt acaacatatg ccttatcgtc
    16021 cacaatgata ttcggtaata atccaacggg taatgcacct tgcgcgatga cattttcaat
    16081 taaactattg gctacttctt catcgattaa tggatgattc agtaaaatgt cgaattgttc
    16141 ttctgataac cattgcttat caaccaattg ttgtaacttt tctttacgag ataaatgtcg
    16201 aaaattctta tctaaatttt gcatggacgt actcctttta cttcacataa tttttaacat
    16261 ttcaatcact actattttta ccacaaaata acgtcattcg tcttaaaatt caattgaata
    16321 attgtcgttt tgactttaaa ataaaacaag gtaaattaaa acgcttacaa gaaacgacaa
    16381 atcattttta aatttagtat atttctttgt ataaaattag catattctga tatgatacaa
    16441 gtgttgcttt tataaatttg aaaggatgta aaaccttatg acaataggta tcgataaaat
    16501 aaacttttac gttccaaagt actatgtaga catggctaaa ttagcagaag cacgccaagt
    16561 agacccaaac aaatttttaa ttggaattgg tcaaactgaa atggctgtta gtcctgtaaa
    16621 ccaagacatc gtttcaatgg gcgctaacgc tgctaaggac attataacag acgaagacaa
    16681 aaagaaaatt ggtatggtaa ttgtggcaac tgaatcagca gttgatgctg ctaaagcagc
    16741 cgctgttcaa attcacaact tattaggtat tcaacctttt gcacgctgct ttgaaatgaa
    16801 agaagcttgt tatgctgcaa caccagcaat tcaattagct aaagattatt tagcaactag
    16861 accgaatgaa aaagtattag ttattgctac agatacagca cgttatggat tgaattcagg
    16921 cggcgagcca acacaaggtg ctggcgcagt tgcgatggtt attgcacata atccaagcat
    16981 tttggcatta aatgaagatg ctgttgctta cactgaagac gtttatgatt tctggcgtcc
    17041 aactggacat aaatatccat tagttgatgg tgcattatct aaagatgctt atatccgctc
    17101 attccaacaa agctggaatg aatacgcaaa acgtcaaggt aagtcgctag ctgacttcgc
    17161 atctctatgc ttccatgttc catttacaaa aatgggtaaa aaggcattag agtcaatcat
    17221 tgataacgct gatgaaacaa ctcaagagcg tttacgttca ggatatgaag atgctgtaga
    17281 ttataaccgt tatgtcggta atatttatac tggatcatta tatttaagcc taatatcatt
    17341 acttgaaaat cgagatttac aagctggtga aacaatcggt ttattcagtt atggctcagg
    17401 ttcagttggt gaattttata gtgcgacatt agttgaaggc tacaaagatc atttagatca
    17461 agctgcacat aaagcattat taaataaccg tactgaagta tctgttgatg catatgaaac
    17521 attcttcaaa cgttttgatg acgttgaatt tgacgaagaa caagatgctg ttcatgaaga
    17581 tcgtcatatt ttctacttat caaatattga aaataacgtt cgtgaatatc acagaccaga
    17641 gtagtcggtg tatttaaaac acatataata aaacctaaaa gcagcagtaa gaccacttct
    17701 aattgaaatc gtcttactgc tgttctctat ttataacact tcgtattgaa tgaattcatt
    17761 atgcctattt gacacattat tgaagttttc ctaatgcctg gatcctttat acgttacggc
    17821 ttcgtgctat gttttggtac ataaagcttt gacatatcga tattctccaa ctctaacagc
    17881 ttaattttat tattaatcgt tccaccgaac cctgttaagc tacccgtttt accgacaaca
    17941 cgatgacatg gcacgataat agataatgga ttacttccga ctgcacctcc aaccgcttgg
    18001 gctgacattt ttggcttgtt aagcagcttg cctacttttt tggcaatagc accatacgtt
    18061 gttagagtcc cataaggaac ctgtcttaat tcattccaaa cacactgttg aaaatgacta
    18121 cctgttggct ttaaaggtat tgtgatttca ggattgtcac ctttaaaata cgcgtctaac
    18181 cactgtgtcg cctctctaaa tatcgctaaa gacgtatttt cttccctagt accatcacct
    18241 tgttgatttt caaacaaaac agcggtcaga cttaccccat cactcaaaag ttccaatcgt
    18301 cctacaggcg aatcatagta actcttatac tccataaaaa tccccccttt ttctaatcat
    18361 acgccttaat tgttaaatta gaaaatattc atgttcacat tttttaaaaa taaataagtc
    18421 gtcaattacg tatataaaca cgtaatacca gctatcactt tgctgcaata tacagttaca
    18481 tatcttacta cacgtgctaa cctcttactt tgtaaaccaa atcttaaatt aaaatattga
    18541 aaatgcaatg aatccttaat attttattaa acctataatt acttattaaa aataacacac
    18601 aatattcata aagttttaaa aatattctgt tttatcacct actattagtg gaaaagtaca
    18661 attgcaattg tatatagttt gcataacgct tcaaaagtaa tttctttttt gtttagttca
    18721 aaaaaattta gaggtgatgt tatatgaata acggtttttt caatagcgac tttgattcaa
    18781 tttttcgaag aatgatgaaa gatatgcaag gttcaaatca agtcggaaac aaaaagtact
    18841 atattaatgg taaagaagtt tcacctgaag aactagcgca actcacacaa caaggtggca
    18901 atcactctgc tgaacaaagt gcgcaagctt ttcaacaagc agcacaaaga caacaagggc
    18961 aacaaggtgg caacggcaat tatttagaac aaattggtcg taaccttacg caagaagcac
    19021 gtgacggttt attagatcca gtcattggtc gtgataaaga aattcaagaa actgctgaag
    19081 ttttaagtag acgaactaaa aacaatccta tattagttgg agaagctggt gttggtaaaa
    19141 ctgcgattgt tgaaggttta gcacaggcaa tcgttgaagg aaatgtacca gcagcaatca
    19201 aagacaaaga aattatttct gtagacattt catcattaga agctggaacg caatatcgtg
    19261 gtgcttttga agaaaatatt caaaaattaa tcgaaggtgt taaatcttca caaaatgccg
    19321 tactattctt tgatgaaatc catcaaatta tcggttcagg tgccacagga agtgattcag
    19381 gtagcaaagg gttatctgat attttgaaac ctgcattaag tcgtggtgag atttctatta
    19441 ttggtgcaac aacacaagat gaatatcgaa acaatattct taaagatgct gcattaacgc
    19501 gcagatttaa tgaagtgctt gttaatgaac caagcgctaa agatactgtt gaaattttaa
    19561 aaggtattcg cgaaaaattc gaagaacacc atcaagtaaa attaccagat gacgtattaa
    19621 aagcatgtgt tgacttatca attcaatata ttccacaacg attattacca gataaagcaa
    19681 tcgatgtgtt agatattaca gcagcacatt tatctgcgca aagtccagct gtcgataaag
    19741 ttgaaactga aaaacgaatt tctgaattag aaaatgataa acgtaaagca gtaagtgctg
    19801 aagaatataa aaaagctgac gacattcaaa atgaaatcaa atcattacaa gataaattag
    19861 aaaatagtaa tggtgaacat actgctgttg ctacagttca tgatatttca gatactattc
    19921 aacgattaac tggtattcca gtttctcaaa tggatgataa cgatattgaa cgtttaaaaa
    19981 atatttctaa tcgtttaaga agtaaaatca taggtcaaga tcaagctgta gaaatggttt
    20041 cacgtgcaat tcgccgtaat cgtgctgggt ttgatgacgg caaccgtcca attggcagtt
    20101 tcctatttgt tggccctact ggtgttggta aaacagagct tgctaaacaa ttagcaattg
    20161 atttatttgg taataaagat gcactgattc gacttgatat gagtgaatat agtgacacaa
    20221 cagctgtttc aaaaatgatt ggtacaactg ctggttatgt tggttatgat gacaattcaa
    20281 atacgttaac tgaaaaagta cgccgtaatc catactcagt cattctattt gatgaaatcg
    20341 aaaaagcaaa tccacaaatt ttaacattgt tattacaagt aatggatgat ggtaatttga
    20401 ctgatggtca aggtaatgtc atcaacttta aaaatacaat tattatttgt acatcaaatg
    20461 ctggctttgg caatggcaat gacgctgaag aaaaagatat tatgcacgaa atgaaaaaat
    20521 tcttccgccc tgaattcctt aaccgcttca acggcatcgt tgaattctta catttagata
    20581 aagatgcatt gcaagatatc gtcaacttat tattagacga tgtacaagtt acattagaca
    20641 aaaaaggtat tacgatggac gtttctcaag atgcgaaaga ttggttaatt gaagaaggct
    20701 atgatgaaga attaggtgca cgtccattaa gacgtattgt tgaacagcaa gtacgtgaca
    20761 aaattacaga ttactattta gatcatacag acgttaaaca tgtggatata gatgttgagg
    20821 ataacgaatt agtcgtaaaa ggtaaataac gacactttaa catatcgcgc atcaaaaatg
    20881 agcatcaggt cgcccttgcc tgtgctcatt tttttaatta tttccctgga aaatgattcg
    20941 ctgtgtgctg ttctgttcca caacaatcac gattaatgtc acatgtacca cattttcctt
    21001 gttttgaacg cttgaaaaat tttactagtg tatataaggc atatccgaaa attgctaaaa
    21061 aaattaaaat gttaataatg actgacactt taaccactcc ttaaacaaat aaatgtccga
    21121 cttgataaaa aatgaatgtt aagacatatg cagtgactag aggataggca actgcaagtg
    21181 ccgtccattt ccatgaataa gtctctttac ggattgctgc tactgtagaa acacaaggaa
    21241 tatacaatag tataaatatc ataaatgcat acgcagatag cggtgtgaat tgattttgaa
    21301 tcacattaac aaggcctgca tcacctgatg aatagataat cgccatcgaa cttacgataa
    21361 cttcttttgc taaaaatcct ggcactaacg tagcacctgc ttgccatgtt ccaaatccga
    21421 gcggttgcac taacatacca aagaaactac caaccatatg taaaaaactt tgattgatat
    21481 tcacattgat accatgtggt cctacataac ttaatagcca aatgactact gagccgccaa
    21541 aaataaatgt acctgcttta cgaacaaagc ccatagcctt ttcccaagta ctacgccaca
    21601 acgttttaat ggaaggcaca cggtatgttg gcaattccac aataaagatt gcattatcat
    21661 tttttaaaat cgtcttagta agtactgtac tgactaaaaa tgccataata atacctaaaa
    21721 catacaggct taatactact aaagattgat tctctttgaa aaagatacct acgaacaacg
    21781 catacactgg cagtctagca gagcatgaca tgaatggtgc aattaatatc gttgttaaac
    21841 gctctttttc attttcaata ctgcgcgcag ccataatact cggtacatta caaccaaatc
    21901 cgataatcat tggtataaaa gacttcccgc ttaaaccgaa cgattccata atacgatcca
    21961 ttaaaaccgc aatacgtgcc atataacccg aatcttctaa taaagatata aagaaaaaga
    22021 gcacaacaat ttgtggtaca aagactaata ctgatcctac accagcaata atgccatctg
    22081 taattaaatc ttgtaaaaat ggtataacac caagataatt cataatcgtc ttcacactat
    22141 ctgtaaatgt accacctata aatgcatcga gttgatccga caaaggtgtg ccaatccatg
    22201 taaatgtagt ttgaaagatc aaccacataa ttgctagaaa gataggcatc cctatatatt
    22261 tatgtgttaa tatcttgtct attctagagc tgaaatattg cttatcttca tctggatacg
    22321 ttaccacgtc ttgcaataac gtctcaatat aatgattgcg tatacgctcc atctctcgac
    22381 gaacagatac agcccctact tgttcagcaa cttgatcacg taaactcgac aatttattta
    22441 caacctctga attaagttcg tttgcaattt gcatgttatt taataagaat tgaatcgcaa
    22501 taaacctagc ttgatactta tcatgagatg tctctgtcat tattatttga cacatatttt
    22561 taattgtctc ttcaatcttc tcaccataat tgattttaaa atgcggttga tacccttccc
    22621 ctagatgctt tatttcgcca agtaaatatt ttgttccttt gcctgtacgt gccacaactg
    22681 gaaaaatagg tgtttttaac tttttcatca atttatgata atcgattttt atcccgcgct
    22741 ttgtagctac atcaatcata tttaatccga tgtatattgg ttgattaagt tctaacaatt
    22801 gtactgttaa ttgcatattt ctttttagtt gactcgcatc aacaatgtta atgattcctg
    22861 aaaatgaatc gtttaataaa tagtctgtca ctacagtttc atctttagaa atcggcgata
    22921 aatcatatgt acctggtaaa tcaattaatt gtcctacatt ttctttaagt ttccctactt
    22981 ttttctctac cgttacgcca ctccagttgc ctatatattc atacgaacca gttaaagcgt
    23041 taaacaaaga tgttttacca acattaggat ttcctaaaat acaataattt tccattcgtc
    23101 cggctcctat tcttctaatg caatagaaca agcatcgcaa tgtctaatac ttaactgttg
    23161 tccgtttact tcaataatac atggcccttt aaataaacat ttttgtttaa tcgttatgat
    23221 agcgtcatct gttaacccaa aggcacttag acgatacaac atattctcat tagcaatatc
    23281 cattcgcttt attttataag ccttattcat ttcaccattt ttaatgttta acatactatt
    23341 tcgctctcct attagaaata ataatcatta tcacttaaaa atcataaccc ttaaaattgt
    23401 agctcgcaat actttattta aataattttc atttttcatg taaaatttgt gacattgcaa
    23461 aaatgttttg tataatcact tccatgcaaa aaacctccgc ttcaaatgtc attgatagac
    23521 atttaaagcg gaggcatgta catgttattt tatgatttat ttcgtagatt tttttctaac
    23581 aattgattga gcttttcaat atttttattc aactgttcta tattcgttgc ttgttttttt
    23641 gaatttagtt gatgcttgtt ttgataatct attaaagcat catataagaa caatgaactt
    23701 gctgattgct gtttcacttc ttggtacaat gccatcgttt tatcatctag tactattgaa
    23761 tgtgcatcat cttgcccatg tgcatcttga tgtttcgata catttgttgc accttgtgga
    23821 tatactttaa aggttgtgtc gtatgtttcc ttactatctt tagcttcaga ttcctgtgat
    23881 tcaaccgttt tatatttttc aagtgcatgt ccttcaatat caactcgtgg aataatgcga
    23941 ttcaaccatg ctggtaaata ccacgaacct tttccaaaca atttcgttaa tgcaggaatt
    24001 aacatcattc tgactacgaa ggcatcaaag agtacaccaa acgctaatgc catacccatt
    24061 gatttaatca tgacatcttc ttggaataca aacgcaaaga agacactaaa cataattaat
    24121 gcagctgcta caataacagg accgctttct ttcaatccta ctttgataga ataatcatta
    24181 tcccctgttt tactatattc ttcatgaatt cgcgacataa ggaagacttc ataatccatc
    24241 gctaatccaa ataagatacc tatagtaata accggtaaaa atgctagcat tggtcctgtc
    24301 gtttcaatac caaacagacc tttcataaaa ccatcttgca ttactaatgt tgtaaatcct
    24361 aatgttgcca ttaatgacaa gacgaatcct aaaactgctt ttaatggtat tagaattgaa
    24421 cggaagacaa tcattaataa gaaaaatgct aatacaacaa tgactgaggc aaataaaggt
    24481 atcgcctcat ttaacttttt agacatatca atattaatga cactttgtcc cgaaatctcc
    24541 gttttgaacc catatttatc ttgtgcatct ttatgataat ctcgtaaatc atgcactaaa
    24601 tcatttgtac tctctgcatt aggcccttgc ttaggtatca cgaccatcaa agcgtaatca
    24661 ttatctttac tcatttgtgg tggcgtaacg atatctacat ttttcttatc tttaatatct
    24721 ttatatacag actgtaaatc ttgttgtaat ccttgtggat catccttttt atctttcaca
    24781 tttatcaaca tcggtatttg gccattaaat ccttcaccaa atttatccga gataatatcg
    24841 taagcttttt tctgtgtaga atctgctggt ttaacaccgt catctggaat accaagtcgc
    24901 atatgactaa ctggtattgc agctgctact aatatgatta aacctagtaa tactgccgca
    24961 agtgcatttc ctgtaataaa tttagaccat ggcgtatcaa tatctttttt gaatttagac
    25021 tgtaatttat tcactttaat gcgtttatgg aaaatgctta ttaatgcagg taataaagtt
    25081 aaagcgctaa gtactgcaaa aacaacacta attgccgaag caaatcccat taccgctaag
    25141 aagtcaatgc ctactaatga taaaccacat actgcaatta caactgttac accagcaaaa
    25201 acaactgcac tacctgctgt tcctattgca agaccaatgc ctttaatgta atctgtttca
    25261 gttttcataa cttgtcgata tctgaataaa ataaataatg cataatcgat accaactgct
    25321 agtccaatca ttacggctaa tgtcagtgtg acatttggta tatcgaatgc ataagttaac
    25381 aaactgataa tacctacacc agaggctaga ccaatcaatg cacttataat tggtaatcct
    25441 gcagcaatga ctgaaccgaa tgtgattaac agtacaacaa atgcaacaat aataccaact
    25501 agttcagaat taccgcctac ttctgtagat gtcattcctg taccagttaa ctctgtttgt
    25561 acattatgat tgtcacgtaa gtctttcaaa tgacttttaa cattatctct agagccatct
    25621 tttaaagatg tttgactaac gtcatatgtg atatctgcaa atgcagttgt tttatcttta
    25681 ctaatttgct tattttcata aggatctgat attttatcaa tgtgcttgtc atctttttta
    25741 atatcatcta acgttttctt aatatcttta gtaatgttcg gttgcacaat accatcatct
    25801 ttagtcgtct taaagacaac acgtatttgt gccttttcac tatcttgatt aaaatgtttt
    25861 tcaatctttt tattcgtatc taacgactct aatcctgtca ttttaatatc attgtcaaat
    25921 ttcggtgcat ttgtagcaag tggtatcaat attgcagcta caatcactat ccatgcaatg
    25981 accgcggacc atttatgttt tgcgatgaat gtccccatct tatataaaaa ttttgccaaa
    26041 gtatattgcc tccttttaaa atcaacgtta tagtttaaat atacagtgta gattattgtt
    26101 cgattatagt atctatcccc gacctcttaa agaatcaatt ggaaaatttt gtatattaaa
    26161 ctacacacaa aggagaaatg tagatgaaag agactgattt acgagttata aagacaaaaa
    26221 aagcattgtc gagtagcttg ctacaattgt tagaacagca attattccaa acgattactg
    26281 tcaatcaaat ttgcgacaac gcactcgtac accgtacaac attttataaa catttttatg
    26341 ataaatatga tcttctagag tacttgttca atcaattgac taaagactac tttgctagag
    26401 atatcagtga ccgtcttaat catccattcc aaacgatgag tgatacgatt aataataaag
    26461 aggatttgag agaaatcgca gaattccaag aagaagacgc tgaatttaat aaagtattaa
    26521 aaaatgtctg cattaaaatt atgcataacg atatcaaaaa taatagagac cgtatcgata
    26581 ttgacagcga catcccagat aatctcatat tttatattta tgactcgttg attgaaggtt
    26641 ttatacattg gataaaagat gaaaaaattg attggcctgg cgaagatatt gataacattt
    26701 tccatagatt aatcaatatt aagattaaat agtagatgag aaactcatga gcgttaccaa
    26761 cattcataat aaaaacgata gtgtacacgt taatgaattc gtgtactact atcgtttttt
    26821 atttttatcg tgcttatcgc tattaaaaca actgatacac aacacataaa ctatgaagaa
    26881 aaaaataaat ccgctatcta aatgactttg actcagttgt ttaaatgacc aaattgctaa
    26941 tacaattccc attattattg aaataacgta tctcacattc ttatacctat aatccttttc
    27001 taaaaatatg gttgctatta cttaattttt aaagttataa ataaaaagag ccaaccgcaa
    27061 tggatggccc ttgttcatta tgaagcatta gaacatttct gaaacaacct tttgttctaa
    27121 gaagtgtaat aagtagtctg gactacctgt tttagcgtcc gtacctgaca ttttgaaacc
    27181 accaaatgga tggtatccaa caactgctga agtacagcct ctgttaaggt ataaattgcc
    27241 tacatcaaat tcgtttaccg ctttaatcca atgctcgcga ttatttgtaa tcactgcacc
    27301 agttaaaccg taatctgtat catttgcaac ctcaattgct tcatcaaaat cgttaacttt
    27361 cacaaagcca acaactggac caaaaatttc ttcttgcatg attctatctt tagatttaag
    27421 tcctgaaatg attgttggtt ctacaaagta accttttgaa tcatcagtgc cgccaccttg
    27481 ttctaattta ccttcttctt taccaatctc aatataattt ttaatcttat caaattgttt
    27541 tttattaata actgggccca tatacgtatt gtctacagta ttgcccaacg ttaattcttt
    27601 tgttaatttg attgatttct ctaatacttc gtcataaacg tctttatgca caattgcacg
    27661 tgaacatgct gaacattttt gaccagaaaa accaaatgct gacgttacaa tagcttctgc
    27721 tgccatatct gtatcaatat tttcatcaac tacaatggca tctttaccac ccatttcagc
    27781 gataacacgt ttcaagaagt tttgaccttc ttgaacaacg gcactacgtt cataaattct
    27841 agtacctgtc gcacgtgatc ctgtaaatgt aacgaaatgc gtatctttat gatcaactaa
    27901 gtaatcacca atttctttcg gatcaccagg aacaaagtta actacgcctt ttggtaatcc
    27961 tgcttcttct aaaatttcca ttaatttata agcgatataa ggtgtatcct cagcaggttt
    28021 caataacact gtattacctg ccacaactgg tgctaaagtt gtaccagcca taatcgcaaa
    28081 cgggaagttc cacggcggaa ttgtaacacc tgtaccaatt gatttataga aatatttatt
    28141 gtgttcacct tcacgatcaa gtactggctt accttgagcc aagtccatca ttgaacgtgc
    28201 atagtattca ataaaatcaa taccttcagc tgcatcacca actgcttcat cccatggctt
    28261 acctgcttca taaaccataa ttgctgcaat ttccgctttt cgacgacgaa taattgccga
    28321 aacacgtaac ataagctctg cacgatcatt tgctgaccat gttttccaag atttataagc
    28381 ttcgtttgct gctttaaacg catcttcaac atcttgtttt gttgcctttg atgcatttgc
    28441 aatcacttgt gatgtgtctg caggattgat tgatttaatt ttgtcatctt tgaaaatctt
    28501 ctctccatta atcactaatg gtatgtcttg acctaattct ttttccacgt ctttcaatgc
    28561 tttcttaaac atatccacat tttcttggac tgaaaaatcg taaccaggtt catttttaaa
    28621 ttctactacc atgtacactt accccctata aattttgaaa gtggtttaac cctttgattt
    28681 aatgatataa catcatttaa actcatttta ctatgattaa ggttagtttt gcaatcgctt
    28741 tcatttttat gttttatcac ttattctcaa gtattttgaa attgattggt tactttttaa
    28801 aatttatatg ggtcgcaact gctactttat cgtttcgtca tttaatgttt cggatggtag
    28861 gtcattatca attttacgaa cgactttaca agggtttcca accgctaagc tgtgtggcgg
    28921 aatatcttta gtgacaacac taccagcacc aatcacactg ccttctccaa tcgtcacccc
    28981 tggtaacacg gctacatgac cgccaaacca agtattactg ccaatatgaa tgggtccggc
    29041 tttttcaaaa ccttcatttc tatgatggaa attaagtgga tgtgtcgctg tgtagaatcc
    29101 acaattaggt cctataaaaa cattatcgcc aattgtaatc tgtccaccat ccataaagta
    29161 acaattggtg tttacataga cattttttcc tagtttaacg ttccaaccat aatctgtatc
    29221 aaaaggaatc gaaatactta cattgtctgt tgttgtttga aataattgat caattaattc
    29281 ctttctttta tttgtagcac tcggtcttgt atgatttaat tcaaagcaaa tatctttcgc
    29341 tcgtgcacgt tcattgatta agtattgatc aaagtttgca tcgtaccatt tttctgctaa
    29401 cattttttct ttttcagtca ttacaccttt caactcctaa taacttattt acttgtttaa
    29461 aagttaatca aataaacctt cgcctatgca actaatacgc tataacatta tgaaatcatg
    29521 accttatcac ccttatctat acaattctcg catcaaatac tgctaaagta gtagataaat
    29581 tcaatactac agacgcattc attttttaat ctattaacgt acaatgtgag taagagaaat
    29641 ataaaggagt atgatagcga tgagaatatt aattacaagc acagttgcta tcttaatcat
    29701 tctaggtttg gtcaaaacga tacaagatta cgaaatgaca aacgacacga gtcgtcagtt
    29761 gtcagacaac aaagatgatg ataaagtcat ccatcttaat aattttaaaa atttacatgc
    29821 gaaagaattt aacccatctg atttctttta agtcacctaa gaattgcaaa tccagaagtc
    29881 atttaagttt tacctttcat tcatacatcc tttaatatta attacgactt cttttatata
    29941 gatgctaagt agagagattg ttgtgcaatg tttgcacggc aatctctctt tttcttttta
    30001 aaattggtaa aagtaaaacg caacgattga cttatatacc tatagggggt acattagacg
    30061 tgtaacaatg aatcacaggg aggcaataat gtggctaata cgaaaaaaac aacattagat
    30121 atcactggta tgacttgtgc cgcatgttca aatcgtatcg aaaagaaact gaataaactt
    30181 gatgacgtta atgcccaagt gaatttaact acagagaaag caactgttga gtataaccct
    30241 gatcaacatg atgtccaaga atttattaat acgattcaac atttaggtta cggtgtcgct
    30301 gtagaaactg tcgaattaga cattacaggt atgacttgtg ctgcatgctc aagccgtatt
    30361 gaaaaagtgt taaataaaat ggacggcgtt caaaatgcaa cggtcaattt aacaacagag
    30421 caagctaaag ttgactatta tcctgaagaa acagatgctg ataaacttgt cactcgcatt
    30481 caaaaattag gttatgacgc gtctattaaa gataacaata aagatcaaac gtcacgcaaa
    30541 gctgaagcgc tacaacataa attgattaag cttatcatat cagcagtatt atctttacca
    30601 ctattaatgt taatgtttgt acatcttttc aatatgcata taccagcact atttacgaat
    30661 ccatggttcc aatttatttt agctacacct gtacaattta ttattggatg gcaattttat
    30721 gtaggtgctt ataaaaactt aagaaatggt ggcgccaata tggatgtact tgttgctgtt
    30781 ggtacaagtg cagcatattt ttacagtatt tatgaaatgg ttcgttggct aaatggctca
    30841 acaacgcaac cgcatttata ctttgaaaca agcgccgtac taattacctt aatcttattc
    30901 ggtaagtatt tagaagctag agcgaagtct caaacaacca atgcgcttgg cgaattatta
    30961 agtttacaag ctaaagaagc acgcatttta aaagatggta atgaagtgat gattcctcta
    31021 aatgaagtac atgttggaga tacacttatc gttaaaccag gtgaaaagat acctgttgat
    31081 ggcaaaatta ttaaaggtat gactgccatc gacgaatcta tgttaacagg tgaatctatc
    31141 cctgttgaga agaatgttga tgatactgta attggttcaa cgatgaacaa aaacggtact
    31201 attactatga cagcaacaaa agttggcggg gacactgcgt tggcaaatat tattaaagtt
    31261 gtcgaagaag ctcaaagttc taaagcgccg attcaacgat tggcagatat tatttctggt
    31321 tatttcgttc ctatcgttgt tggtatcgca ctattaacat ttatcgtgtg gattacttta
    31381 gttacaccag gtacatttga acctgcactt gttgcgagta tttccgttct cgtcattgct
    31441 tgtccatgcg cattgggact tgctacacca acttctatta tggtaggtac tggtcgcgct
    31501 gctgaaaatg gtattttatt taaaggtggc gagtttgttg aacgcacaca tcaaattgat
    31561 accatcgttt tagataagac gggtaccatt acaaatggtc gtccagtcgt gacagattat
    31621 catggtgaca atcaaacgct acaactactt gctactgctg aaaaagattc tgaacaccca
    31681 ttggcagaag ccattgtcaa ttatgcaaaa gaaaagcaat taatattaac tgagacaaca
    31741 acatttaaag cagtacctgg ccatggtatt gaagcaacga ttgatcatca ctatatattg
    31801 gttggtaacc gtaaattaat ggctgacaat gatattagct tgcctaagca tatttctgat
    31861 gatttaacac attatgaacg agatggtaaa actgctatgc tcattgctgt taattattca
    31921 ttaactggta tcatcgcagt ggcagatact gtcaaagatc atgccaaaga tgctataaaa
    31981 caattgcatg atatgggcat tgaagttgcc atgttaactg gcgataataa aaacactgct
    32041 caagccattg caaaacaagt aggcatagat actgttattg cagatatttt accagaagaa
    32101 aaagctgcac aaattgcgaa actacagcaa caaggtaaga aggttgcgat ggttggtgac
    32161 ggtgtaaatg atgcacctgc attagttaaa gctgatatcg gtatcgccat tggtacaggt
    32221 acagaagttg ccattgaagc agctgatatt actattcttg gtggcgactt gatgcttatt
    32281 cctaaagcca tttatgcaag taaagcaacc attcgtaata ttcgtcaaaa tctattttgg
    32341 gcattcggct ataatattgc cggtatccct atagctgcat tgggcttact tgcgccatgg
    32401 gttgctggtg ctgcaatggc actaagttca gtaagtgttg tcacaaacgc acttagattg
    32461 aaaaagatgc gattagaacc acgccgtaaa gatgcctaga ttccttaata atgaaggatt
    32521 cgttggtgat tctgagatag gctagtgatt ggctctataa tgtcgcggtt tacagttgga
    32581 tc
//
LOCUS       DALAGD010000022        32496 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.022, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000022 DALAGD010000000
VERSION     DALAGD010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 32496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32496
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.022"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(200..1129)
                     /locus_tag="O2701_000793"
     CDS             complement(200..1129)
                     /locus_tag="O2701_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCZ4292699.1"
                     /translation="MKKLVPLLLALLLLVAACGTGGKQSSDKSNGKLKVVTTNSILYD
                     MAKNVGGDNVDIHSIVPVGQDPHEYEVKPKDIKKLTDADVILYNGLNLETGNGWFEKA
                     LEQAGKSLKDKKVIAVSKDVKPIYLNGEEGNKDKQDPHAWLSLDNGIKYVKTIQQTFI
                     DNDKKHKADYEKQGNKYIAQLEKLNNDSKDKFNDIPKEQRAMITSEGAFKYFSKQYGI
                     TPGYIWEINTEKQGTPEQMRQAIEFVKKHKLKHLLVETSVDKKAMESLSEETKKDIFG
                     EVYTDSIGKEGTKGDSYYKMMKSNIETVHGSMK"
     gene            complement(1126..1962)
                     /locus_tag="O2701_000794"
     CDS             complement(1126..1962)
                     /locus_tag="O2701_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCZ4292700.1"
                     /translation="MLEFVEHLFTYQFLNRALITSIIVGIVCGTVGSLIVLRGLSLMG
                     DAMSHAVLPGVALSFLFGIPMFVGALITGMIASIFIGYITSSSKTKPDAAIGISFTAF
                     LASGIIIISLINTTTDLYHILFGNLLAITNSAFLTTIVIGSIVLILIIIFYRPLMIST
                     FDPTFSRMSGLNTTLLHYFVMLLLSLVTVASIQTVGIILVVALLITPASTAFLISKKL
                     YSMMIIASLISVISSIVGLYYSYIYNIPSGATIVLCTFVIYIITLFFTKFTNRKKRGS
                     LT"
     gene            complement(1956..2699)
                     /locus_tag="O2701_000795"
     CDS             complement(1956..2699)
                     /locus_tag="O2701_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCZ4292701.1"
                     /translation="MLETKDLNLFLGNKHVLKNISLSIPVRGEIIGIMGPNGAGKSSL
                     IKSLIGEFNATGTKLLYNKPIQQQLQHITYIPQKAHIDLDFPISVEQVILSGCYKEIG
                     WFRRPNKSARDKLKQLLSDLELESLRHRQISELSGGQLQRVLVARALMSESEVYFLDE
                     PFVGIDFSSEKLIMTKIENLKQQGKLILIIHHDLSKAKQYFDRIILLNQTLRYFGDSE
                     EAMSVTRLNETFMSSTDCSDPSQRSNITC"
     gene            2821..3465
                     /locus_tag="O2701_000796"
     CDS             2821..3465
                     /locus_tag="O2701_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HCZ4292702.1"
                     /translation="MLTEEKEDYLKAILTNNGDKNFVTNKILSQFLNIKPPSVSEMVG
                     RLEKAGYVETKPYKGVRLTEDGLTHTLDIIKRHRLLELFLIEILKYNWEEVHQEAEIL
                     EHRISDLFVERLDSLLNFPETCPHGGVIPRNNEYKEKYITTILNYEPGDIVTIKRVRD
                     KTDLLIYLSSKDISIGNEVEIVSKDEMNKVIIIKRNDNVIIVSYENAMNMFAEK"
     gene            complement(3545..4297)
                     /locus_tag="O2701_000797"
     CDS             complement(3545..4297)
                     /locus_tag="O2701_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HCZ4292703.1"
                     /translation="MNYLSNFFNTTIQLNIYIIVIVAACYIAIHQYRHKPVLNYLDVI
                     LNYIPVLTHEFGHVLFNKLAGGRAKDLVIVTSPRERQQTLQQGFAITQSRHLAGQWLT
                     TIGGYFMPPIMLLIGLASSHYQIPSFFIFTYLLIFIYFLILTSRKGSPIVVITLISIM
                     LYFILKDENIVEIQLLVTMSYQYILGVLLGEVLQSSWTIAKLTFQRPSPQWDGSALKE
                     LSHVPIFIYSTIWIIFNLYAVNILLKYTHLIN"
     gene            4533..5297
                     /locus_tag="O2701_000798"
     CDS             4533..5297
                     /locus_tag="O2701_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HCZ4292704.1"
                     /translation="MTVEERSNTAKVDILGVDFDNTTMLQMVENIKTFFANQSTNNLF
                     IVTANPEIVNYATTHQAYLELINQASYIVADGTGVVKASHRLKQPLAHRIPGIELMDE
                     CLKIAHVNHQKVFLLGATNEVVEAAQYALQQRYPNISFAHHHGYIDLEDETVVKRIKL
                     FKPDYIFVGMGFPKQEEWIMTHENQFESTVMMGVGGSLEVFAGAKKRAPYIFRKLNIE
                     WIYRALIDWKRIGRLKSIPIFMYKIAKAKRKIKKAK"
     gene            complement(5358..6152)
                     /gene="tagH"
                     /locus_tag="O2701_000799"
     CDS             complement(5358..6152)
                     /gene="tagH"
                     /locus_tag="O2701_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter
                     ATP-binding subunit TagH"
                     /protein_id="HCZ4292705.1"
                     /translation="MNVSVNIKNVTKEYRIYRTNKERMKDALIPKHKNKTFFALDDIS
                     LKAYEGDVIGLVGINGSGKSTLSNIIGGSLSPTVGKVDRNGEVSVIAISAGLSGQLTG
                     IENIEFKMLCMGFKRKEIKAMTPKIIEFSELGEFIYQPVKKYSSGMRAKLGFSINITV
                     NPDILVIDEALSVGDQTFAQKCLDKIYEFKEQNKTIFFVSHNLGQVRQFCTKIAWIEG
                     GKLKDYGELDDVLPKYEAFLNDFKKKSKAEQKEFRNKLDESRFVIK"
     gene            6499..7311
                     /gene="tagG"
                     /locus_tag="O2701_000800"
     CDS             6499..7311
                     /gene="tagG"
                     /locus_tag="O2701_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter permease
                     subunit TagG"
                     /protein_id="HCZ4292706.1"
                     /translation="MSAIGTVFKEHVKNFYLIQRLAQFQVKIINHSNYLGVAWELINP
                     VMQIMVYWMVFGLGIRSNAPIHGVPFVYWLLVGISMWFFINQGILEGTKAITQKFNQV
                     SKMNFPLSIIPTYIVTSRFYGHLGLLLLVIIACMFTGIYPSIHIIQLLIYVPFCFFLT
                     ASVTLLTSTLGVLVRDTQMLMQAILRILFYFSPILWLPKNHGISGLIHEMMKYNPVYF
                     IAESYRAAILYHEWYFMDHWKLMLYNFGIVAIFFAIGAYLHMKYRDQFADFL"
     gene            7410..8513
                     /locus_tag="O2701_000801"
     CDS             7410..8513
                     /locus_tag="O2701_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCZ4292707.1"
                     /translation="MNVLIKKFYHLVVRILSKMITPQVIDKPHIVFMMTFPEDIKPII
                     KALNNSSYQKTVLTTPKQAPYLSELSDDVDVIEMTNRTLVKQIKALKSAQMIIIDNYY
                     LLLGGYNKTSNQHIVQTWHASGALKNFGLTDHQVDVSDKAMVQQYRKVYQATDFYLVG
                     CEQMSQCFKQSLGATEEQMLYFGLPRINKYYTADRATVKAELKDKYGITNKLVLYVPT
                     YREDKADNRAIDKAYFEKCLPGYTLINKLHPSIEDSDIDDVSSIDTSTLMLMSDIIIS
                     DYSSLPIEASLLDIPTIFYVYDEGTYDQVRGLNQFYKAIPDSYKVYTEEDLIMTIQEK
                     EHLLSPLFKDWHKYNTDKSLHQLTEYIDKMVTK"
     gene            8510..9571
                     /locus_tag="O2701_000802"
     CDS             8510..9571
                     /locus_tag="O2701_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCZ4292708.1"
                     /translation="MRFTIIIPTCNNEATIRQLLISIESKEHYRILCIDGGSTDQIIP
                     MIERLQRELKHISLIQLQNASIATCINKGLMDIKMTDPHDSDAFMVIKPTSIVLPGKL
                     DRLTAAFKNNDNIDMVIGQRAYNYHGEWKLKSADEFIKDNRIVTLTEQPDLLSMMSFD
                     GKLFSAKFAELQCDETLANTYNHAILVKAMQKATDIHLVSQMIVGDNDIDTHATSNEE
                     DFNRYITEIMKIRQRVMEMLLLPEQRLLYSDMVDRILFNNSLKYYMNEHPAVTHTTIQ
                     LVKDYIMSMQHSDYVSQNMFDIINTVEFIGENWDREIYELWRQTLIQVGINRPTYKKF
                     LIQLKGRKFAHRTKSMLKR"
     gene            9634..10032
                     /gene="tagD"
                     /locus_tag="O2701_000803"
     CDS             9634..10032
                     /gene="tagD"
                     /locus_tag="O2701_000803"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HCZ4292709.1"
                     /translation="MKRVITYGTYDLLHYGHIELLRRAREMGDYLIVALSTDEFNQIK
                     HKKSYYDYEQRKMMLESIRYVDLVIPEKGWGQKEDDVEKFDVDVFVMGHDWEGEFDFL
                     KDKCEVIYLKRTEGISTTKIKQELYGKDAK"
     gene            complement(10149..11444)
                     /gene="pbp4"
                     /locus_tag="O2701_000804"
     CDS             complement(10149..11444)
                     /gene="pbp4"
                     /locus_tag="O2701_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP4"
                     /protein_id="HCZ4292710.1"
                     /translation="MKNLISIIIILCLTLSIMTPYAQATNSDVTPVQAANQYGYAGLS
                     AAYEPTSAVNVSQTGQLLYQYNIDTKWNPASMTKLMTMYLTLEAVNKGQLSLDDTVTM
                     TNKEYIMSTLPELSNTKLYPGQVWTIADLLQITVSNSSNAAALILAKKVSKNTSDFVD
                     LMNNKAKAIGMKNTHFVNPTGAENSRLRTFAPTKYKDQERTVTTARDYAILDLHVIKE
                     TPKILDFTKQLAPTTHAVTYYTFNFSLEGAKMSLPGTDGLKTGSSDTANYNHTITTKR
                     GKFRINQVIMGAGDYKNLGGEKQRNMMGNALMERSFDQYKYVKILSKGEQRINGKKYY
                     VENDLYDVLPSDFSKKDYKLVVEDGKVHADYPREFINKDYGPPTVEVHQPIIQKANTV
                     AKSMWEEHPLFTIIGGTCLVAGLALIVHMIINRLFRKRK"
     gene            11865..13592
                     /locus_tag="O2701_000805"
     CDS             11865..13592
                     /locus_tag="O2701_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4292711.1"
                     /translation="MKRENPLFFLFKKLSWPVGLIVAAITISSLGSLSGLLVPLFTGR
                     IVDKFSVSHINWNLIALFGGIFVINALLSGLGLYLLSKIGEKIIYAIRSVLWEHIIQL
                     KMPFFDKNESGQLMSRLTDDTKVINEFISQKLPNLLPSIVTLVGSLIMLFILDWKMTL
                     LTFITIPIFVLIMIPLGRIMQKISTSTQSEIANFSGLLGRVLTEMRLVKISNTERLEL
                     DNAHKNLNEIYKLGLKQAKIAAVVQPISGIVMLLTIAIILGFGALEIATGAITAGTLI
                     AMIFYVIQLSMPLINLSTLVTDYKKAVGASSRIYEIMQEPIEPTEALEDSENVLIDDG
                     VLSFEHVDFKYDVKKILDDVSFQIPQGQVSAFVGPSGSGKSTIFNLIERMYEIESGDI
                     KYGLESVYDIPLSKWRRKIGYVMQSNSMMSGTIRDNILYGINRHVSDEELINYAKLAN
                     CHDFIMQFDEGYDTLVGERGLKLSGGQRQRIDIARSFVKNPDILLLDEATANLDSESE
                     LKIQEALETLMEGRTTIVIAHRLSTIKKAGQIIFLDKGQVTGKGTHSELMASHAKYKN
                     FVVSQKLTD"
     gene            13951..15180
                     /locus_tag="O2701_000806"
     CDS             13951..15180
                     /locus_tag="O2701_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCZ4292712.1"
                     /translation="MFLLINIIGLIVFLGIAVLFSRDRKNIQWQSIGILVVLNLFLAW
                     FFIYFDWGQKAVRGAANGIAWVVQSAHAGTGFAFASLTNVKMMDMAVAALFPILLIVP
                     LFDILMYFNILPKIIGGIGWLLAKVTRQPKFESFFGIEMMFLGNTEALAVSSEQLKRM
                     NEMRVLTIAMMSMSSVSGAIVGAYVQMVPGELVLTAIPLNIVNAIIVSCLLNPVSVEE
                     KEDIIYSLKNNEVERQPFFSFLGDSVLAAGKLVLIIIAFVISFVALADLFDRFINLIT
                     GLIAGWIGIKGSFGLNQILGVFMYPFALLLGLPYDEAWLVAQQMAKKIVTNEFVVMGE
                     ISKDIASYTPHHRAVITTFLISFANFSTIGMIIGTLKGIVDKKTSDFVSKYVPMMLLS
                     GILVSLLTAAFVGLFAW"
     gene            15435..15527
                     /locus_tag="O2701_000807"
     CDS             15435..15527
                     /locus_tag="O2701_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292713.1"
                     /translation="MFCYYKQHKGDNFSIEEVKNIIADNEMKVN"
     gene            15705..16538
                     /locus_tag="O2701_000808"
     CDS             15705..16538
                     /locus_tag="O2701_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCZ4292714.1"
                     /translation="MNKTVKDLILVVLGSFIFAAGVNAFIISGNLGEGGVTGLAIILY
                     YAFHISPAITNFLVNAVLIAIGYKFLSKRSMYLTILVTILISIFLSLTESWQVETGNS
                     IVNAIFGGVSVGLGIGVIILAGGTTAGTTILARIATKYLDVSTPYALLFFDMIVVAIS
                     LTVIPLDKVLVTVISLYIGTKVMEYVIEGLNTKKAMTIISTNPDKLAKAIDEQIGRGL
                     TILNGHGYYTREEKDVLYVVISKTQVSKAKRLIKQIDKDAFLVIHDVRDVYGNGFLAD
                     E"
     gene            16820..17617
                     /locus_tag="O2701_000809"
     CDS             16820..17617
                     /locus_tag="O2701_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4292715.1"
                     /translation="MNRLHGQQVKIGYGDNTIINKLDVEIPDGKVTSIIGPNGCGKST
                     LLKALSRLLAVKEGEVFLDGENIHTQSTKEIAKKIAILPQSPEVADGLTVGELVSYGR
                     FPHQKGFGRLTAEDKKEIDWAMEVTGTDTFRHRSINDLSGGQRQRVWIAMALAQRTDI
                     IFLDEPTTYLDICHQLEILELVQKLNQEQGCTIVMVLHDINQAIRFSDHLIAMKEGDI
                     IATGSTEDVLTQEILEKVFNIDVVLSKDPKTGKPLLVTYDLCRRAYS"
     gene            17653..18657
                     /locus_tag="O2701_000810"
     CDS             17653..18657
                     /locus_tag="O2701_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCZ4292716.1"
                     /translation="MTNRENPTPLKFLSYIIGLSMILLITLFISTLIGDAKIQASTII
                     EAIFNYNPSNQQQNIINEIRIPRNIAAVIVGMALAVSGAIIQGVTRNGLADPALIGLN
                     SGASFALALTYAVLPNTSFLILMFAGFLGAILGGAIVLMIGRSRRDGFNPMRIILAGA
                     AVSAMLTALSQGIALAFRLNQTVTFWTAGGVSGTTWSHLKWAIPLIGIALFIILTISK
                     QLTILNLGESLAKGLGQNVTMIRGICLIIAMILAGIAVAIAGQVAFVGLMVPHIARFL
                     IGTDYAKILPLTALLGGILVLVADVIARYLGEAPVGAIISFIGVPYFLYLVKKGGRSI
                     "
     gene            18654..19670
                     /locus_tag="O2701_000811"
     CDS             18654..19670
                     /locus_tag="O2701_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCZ4292717.1"
                     /translation="MISSNNKRRQLIALAVFSILLFLGCTWSITSGEYNIPVERFFKT
                     LIGQGDAIDKLILLDFRLPRMMITILAGAALSISGAIVQSVTKNPIAEPGILGINAGG
                     GFAIALFIAIGKINADNFVYVLPLISILGGITTALIIFIFSFNKNEGVTPASMVLIGV
                     GLQTALYGGSITIMSKFDDKQSDFIAAWFAGNIWGDEWPFVIAFLPWVLIIIPYLLFK
                     SNTLNIIHTGDNIARGLGVRLSRERLILFFIAVMLSSAAVAVAGSISFIGLMGPHIAK
                     RIVGPRHQLFLPIAILVGACLLVIADTIGKIVLQPGGVPAGIVVAIIGAPYFLYLMYK
                     TKNV"
     gene            19904..20872
                     /gene="dhaK"
                     /locus_tag="O2701_000812"
     CDS             19904..20872
                     /gene="dhaK"
                     /locus_tag="O2701_000812"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCZ4292718.1"
                     /translation="MMKKLINKKETFLTDMLEGLLIAHPELDLIANTVIVKKAKKEHG
                     VAIVSGGGSGHEPAHAGFVAEGMLDAAVCGEVFTSPTPDKILEAIKAVDTGDGVLLVV
                     KNYAGDVMNFEMAQELAEMEGINVQTVIVRDDIAVTNEVQRRGVAGTVFVHKLAGYLA
                     EKGYSLTEIKSRVEALLPEIKSIGMAIEPPLVPTTGKYGFDIEDDKMEIGIGIHGEKG
                     IHREEVKDIDHIVGTLLDELYKEVTANDVILMVNGMGGTPLSELNIVTKYIQQNLAAR
                     TVNVAKWFVGDYMTSLDMQGFSITIVPNKPEYLEAFLAPTTSQYFK"
     gene            20914..21498
                     /gene="dhaL"
                     /locus_tag="O2701_000813"
     CDS             20914..21498
                     /gene="dhaL"
                     /locus_tag="O2701_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HCZ4292719.1"
                     /translation="MKVNDMKARLLNLEETFKKHESELTELDRAIGDGDHGVNMVRGF
                     SSLKDKLDDSSMQSLFKSTGMALMSNVGGASGPLYGFSFVKMSAVTKDDMDNQDFITL
                     IQAFAEAVESRGKVTLNEKTMYDVVARAAEKLKNGETLTFNDLQQLADNTKDMVATKG
                     RAAYFGEESKGYIDPGAQSMVYILNALIGDEDNA"
     gene            21491..21853
                     /locus_tag="O2701_000814"
     CDS             21491..21853
                     /locus_tag="O2701_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HCZ4292720.1"
                     /translation="MPKIILVSHSKEIASGTKSLLKQMAGDVDIIPIGGLPDGSIGTS
                     FDIIQEVLTKLEDDALCFYDIGSSEMNVDMAIEMYDGNHRVLKVDAPIVEGSFIAAVK
                     LSIGGSIDDALAEIKQSF"
     gene            21969..22466
                     /locus_tag="O2701_000815"
     CDS             21969..22466
                     /locus_tag="O2701_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292721.1"
                     /translation="MQHLIKKHVLNGEFDLVRQLMSETDFMEFEEAYISSAHEVESMM
                     FYTCILDMIKYEESSEMHDLAFLLLVYPLSEYEGALDSAYYHADASIKLTDGKEVKSL
                     LQMLLLHAIPTPVISDKKAFDIAKQILKLDPNNNVARNVLKDTAKRMDNVVVDINELH
                     QRNAR"
     gene            22922..23989
                     /locus_tag="O2701_000816"
     CDS             22922..23989
                     /locus_tag="O2701_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292722.1"
                     /translation="MMMNKEATKIGFAYVGIVVGAGFSTGQEVMQFFTKYGLWAYLGV
                     IISGFILAFIGRQVAKIGTAFEATNHESTLQYVFGEKFSKVFDYILIFFLFGIAVTMI
                     AGAGATFEESYNIPTWLGALIMTLAIYITLLLDFNKIVRALGIVTPFLIVLVVLIAGV
                     YLFKGHVSLAEVNQVVPEASIWKGIWFGTIYGGLAFSVGFSTIVAIGGDTEKRTVSGA
                     GAMYGGIIYTVLLALINFALQSEYPTIKNASIPTLTLANNIHPLIATVLSVIMLAVMY
                     NTILGLMYSFAARFTEPYSKNYHIFIIIMMVAGYLLSFVGFAELINKLYTIMGYVGLF
                     IVVAVIIKYFKRKNADKKHIA"
     gene            24140..25183
                     /locus_tag="O2701_000817"
     CDS             24140..25183
                     /locus_tag="O2701_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCZ4292723.1"
                     /translation="MNKDNKWTMITALFITVISVLLAFHLKQHYDQITNENHANKDKI
                     NIKNKNVRIYQNLTYNRVFPNSKLDIITPVDMSSNAKLPVIFWMHGGGYIAGDKQYKN
                     PLLAKIAEQGYIVVNVNYALAPQYKYPTPLIQMNQATQFIKENKMNLPIDFNQVIIGG
                     DSAGAQLASQFTAIQTNDRLREAMKFDQSFKPSQIKGAILFGGFYNMQTVRETEFPRI
                     QLFMKSYTGEEDWEKSFKNISQMSTVKQSTKNYPPTFLSVGDSDPFESQNIEFSKKLQ
                     ELNVPVDTLFYDGTHHLHHQYQFHLNKPESIDNIKKVLLFLSRNTSSSGIQTEEKPQI
                     ENPSNELPLNPLN"
     gene            25512..25940
                     /locus_tag="O2701_000818"
     CDS             25512..25940
                     /locus_tag="O2701_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292724.1"
                     /translation="MTRSALKPFKNKRVMVTGRIQRVLFKNYLDRHSTFKPNVRILLK
                     DVFVSGVSIDHLWLYETNKYYALAMELIHQRVKFSANVVPYYKINRNNNLFVQDYGIK
                     RKGRLITEEAYNQNNQYQDKIYEKLPDIDFRLEDFYSKEN"
     gene            complement(26149..26655)
                     /locus_tag="O2701_000819"
     CDS             complement(26149..26655)
                     /locus_tag="O2701_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4292725.1"
                     /translation="MAHIIRRVSIKDVENFISMLANIYDESPYMFYTPGEYDPSVTSA
                     SKQLEEYITSPHKVIFVAESDEQLVGFAFVNTTPFQRIKHVAKIDLGVKKLYQHRGIG
                     QALLDAIMAWCLNNQIHRIEANVPLNNQPALELFKSADFQIEGVLKDKLFIDGKYYDD
                     YMMAKILN"
     gene            26768..27691
                     /gene="graX"
                     /locus_tag="O2701_000820"
                     /gene_synonym="apsX"
     CDS             26768..27691
                     /gene="graX"
                     /locus_tag="O2701_000820"
                     /gene_synonym="apsX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="auxiliary protein GraX/ApsX"
                     /protein_id="HCZ4292726.1"
                     /translation="MKPKVLLAGGTGYIGKYLSEVIENDAELFAISKYPDNKKTDDVE
                     MTWIQCDIFHYEQVVAAMNQIDIAVFFIDPTKNSAKITQSSARDLTLIAADNFGRAAA
                     INQVKKVIYIPGSRYDNETIERLGAYGTPVETTNLVFKRSLVNVELQVSKYDDVRSTM
                     KVVLPKGWTLKNVVNHFIAWMGYTKGTFVKTEKSHDQFKIYIKNKVRPLAVFKIEETA
                     DGIITLILLSGSLVKKYTVNQGKLEFRLIKESAVVYIHLYDYIPRLFWPIYYFIQAPM
                     QKMMIHGFEVDCRIKDFQSRLKSGENMKYTK"
     gene            27707..28381
                     /gene="graR"
                     /locus_tag="O2701_000821"
                     /gene_synonym="apsR"
     CDS             27707..28381
                     /gene="graR"
                     /locus_tag="O2701_000821"
                     /gene_synonym="apsR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor
                     GraR/ApsR"
                     /protein_id="HCZ4292727.1"
                     /translation="MQILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFN
                     PEIVILDVQLPKYDGFYWCRKMREVSNVPILFLSSRDNPMDQVMSMELGADDYMQKPF
                     YTNVLIAKLQAIYRRVYEFTAEEKRTLTWQDAVVDLSKDSIQKGDQTIFLSKTEMIIL
                     EILITKKNQIVSRDTIITALWDDEAFVSDNTLTVNVNRLRKKLSEISMDSAIETKVGK
                     GYMAHE"
     gene            28374..29414
                     /gene="graS"
                     /locus_tag="O2701_000822"
                     /gene_synonym="apsS"
     CDS             28374..29414
                     /gene="graS"
                     /locus_tag="O2701_000822"
                     /gene_synonym="apsS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase GraS/ApsS"
                     /protein_id="HCZ4292728.1"
                     /translation="MNNLKWVAYFLKSRMNWIFWILFLNFLMLGISLIDYDFPIDSLF
                     YIVSLNLSLTMIFLLLTYFKEVKLYKHFDKDKEIEEIKHKDLAETPFQRHTVDYLYRQ
                     ISAHKEKVVEQQLQLNMHEQTITEFVHDIKTPVTAMKLLIDQEKNQERKQALLYEWSR
                     INSMLDTQLYITRLESQRKDMYFDYVSLKRMVIDEIQLTRHISQVKGIGFDVDFKVDD
                     YVYTDIKWCRMIIRQILSNALKYSENFNIEIGTELNDQHVSLYIKDYGRGISKKDMPR
                     IFERGFTSTANRNETTSSGMGLYLVNSVKDQLGIHLQVTSTVGKGTTVRLIFPLQNEI
                     VERMSEVTNLSF"
     gene            29558..30319
                     /gene="vraF"
                     /locus_tag="O2701_000823"
     CDS             29558..30319
                     /gene="vraF"
                     /locus_tag="O2701_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein VraF"
                     /protein_id="HCZ4292729.1"
                     /translation="MAILEVKQLTKIYGTKKMAQEVLRDINMSIEEGEFIAIMGPSGS
                     GKTTLLNVLSSIDYISQGSITLKGKKLEKLSNKELSDIRKHDIGFIFQEYNLLHTLTV
                     KENIMLPLTVQKLDKEHMLNRYEKVAEALNILDISDKYPSELSGGQRQRTSAARAFIT
                     LPSIIFADEPTGALDSKSTQDLLKRLTRMNEAFKSTIIMVTHDPVAASYANRVVMLKD
                     GQIFTELYQGDDDKHTFFKEIIRVQSVLGGVNYDL"
     gene            30309..32198
                     /gene="vraG"
                     /locus_tag="O2701_000824"
     CDS             30309..32198
                     /gene="vraG"
                     /locus_tag="O2701_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease VraG"
                     /protein_id="HCZ4292730.1"
                     /translation="MTFNEIIFKNFRQNLSHYAIYLFSLITSVVLYFSFVALKYAHKL
                     NMTESYPIIKEGSQVGSYFLFFIIIAFLLYANVLFIKRRSYELALYQTLGLSKFNIIY
                     ILMLEQLLIFIITAILGIIIGIFGSKLLLMIVFTLLGIKEKVPIIFSLRAVFETLMLI
                     GVAYFLTSAQNFILVFKQSISQMSKNNQVKETNHNKITFEEVVLGILGIVLITTGYYL
                     SLNIVQYYDSIGTLMFILLSTVIGAYLFFKSSVSLVFKMVKKFRKGVISVNDVMFSSS
                     IMYRIKKNAFSLTVMAIISAITVSVLCFAAISRASLSSEIKYTAPHDVTIKDQQKANQ
                     LASELNNQKIPHFYNYKEVIHTKLYKDNLFDVKAKEPYNVTITSDKYIPNTDLKRGQA
                     DLFVAEGSIKDLVKHKKHGKAIIGTKKHHVNIKLRKDINKIYFMTDVDLGGPTFVLND
                     KDYQEIRKYTKAKHIVSQFGFDLKHKKDALALEKAKNKVDKSIETRSEAISSISSLTG
                     ILLFVTSFLGITFLIAVCCIIYIKQIDETEDELENYSILRKLGFTQKDMARGLKFKIM
                     FNFGLPLVIALSHAYFTSLAYMKLMGTTNQIPVFIVMGLYICMYAVFAVTAYNHSKRT
                     IRHSI"
BASE COUNT        11301 a         4539 c         5782 g        10874 t
ORIGIN      
        1 agagaagttc taataccacc atatcgtgcg atcgggaacg gtatatatat taataggagg
       61 gtaatatata tttaacgcac gatatgggac tattagcctt cgactttgtt atgttgatgt
      121 gtggcctaaa atattggaga taccaatatt ttaggttgca tcaacatcaa ttcatcttac
      181 ttcattaaaa cacagcgtgt tatttcatgc ttccgtgtac agtttcaata tttgatttca
      241 tcattttgta gtaagagtca cctttagtgc cttctttacc gattgaatct gtgtacactt
      301 caccaaagat atctttcttc gtttcttcag ataaactttc cattgctttc ttatcaacac
      361 ttgtttctac taataagtgt tttaatttgt gctttttaac aaactcaata gcttgtctca
      421 tttgttcagg tgtaccttgt ttttcagtgt taatttccca aatataacct ggtgtaatac
      481 cgtattgttt tgagaagtac ttgaaggcac cttcacttgt aatcatggca cgttgttctt
      541 ttggaatgtc attaaatttg tctttactgt cattatttaa tttttccaat tgagcaatgt
      601 atttgttacc ttgcttttca taatctgctt tatgtttttt gtcgttatcg ataaatgttt
      661 gttgaattgt ttttacgtat ttaataccgt tatctaaact taaccatgcg tgtggatctt
      721 gtttatcttt gttgccttct tcaccgttta aatagatagg tttaacatct tttgatactg
      781 cgataacttt tttatctttt aatgatttac cagcctgttc taaggctttt tcaaaccaac
      841 cgttaccagt ctctaaattt aatccgttgt ataaaataac gtcagcgtca gttaactttt
      901 taatatcttt aggtttaact tcatattcat gaggatcttg accaacaggt acaatactat
      961 gaatatcgac gttgtctcca ccaacatttt tagccatatc atataaaatt gaattcgtcg
     1021 ttactacttt taatttgcca tttgacttat cactgctttg tttaccacca gtaccacatg
     1081 cagcaactag aagtaataag gctaataata aaggtactaa ttttttcatg ttaaacttcc
     1141 tcgtttcttt ctattcgtaa attttgtgaa aaataatgtg atgatataaa ttacaaacgt
     1201 acaaagtacg attgtcgcac cactaggaat gttgtaaata tagctgtaat aaagtccgac
     1261 aattgaactt atgacactta ttaaacttgc tataatcatc attgagtata gttttttact
     1321 aattaaaaat gctgtagatg caggtgtaat taataatgca actacaagaa taatacctac
     1381 cgtttgaata cttgctactg ttactaatga gagtaacaac atcacaaagt aatgtaataa
     1441 cgtcgtattt agaccactca ttctactaaa cgttggatcg aatgtagaaa tcattaatgg
     1501 acgatagaaa ataatgatta gaataaggac gattgaacca atcacaatag ttgttaaaaa
     1561 tgcactattt gtgattgcca gtaaattacc aaacagaata tggtacaaat ctgtcgtagt
     1621 gtttattaag ctaataataa taatccccga agctaagaaa gcggtaaaac taattccaat
     1681 agcggcgtca ggtttcgttt tactactaga tgtgatataa ccgataaaaa tacttgcgat
     1741 cataccagtt ataagtgcgc ctacaaacat tggaatacca aataagaatg atagggcaac
     1801 accaggtaat actgcgtgac tcattgcatc tcccattaat gaaagaccac gtaatacaat
     1861 taaactacca actgtaccac aaactatccc tacaataatt gaagttatca atgctcgatt
     1921 caagaattga tatgtaaata aatgttcgac aaactctaac atgttatatt gctcctttga
     1981 ctagggtcac tacagtcagt gctactcata aatgtttcgt ttaagcgagt gacactcata
     2041 gcctcttcac tatcaccaaa gtatcgtaat gtttgattta atagaataat gcgatcaaag
     2101 tattgctttg cttttgatag atcatggtgg atgataagaa taagttttcc ttgttgtttt
     2161 aagttctcga tttttgtcat gattaatttt tcgctactaa aatcaattcc gacaaacggc
     2221 tcatctagaa aataaacttc actttcggac atcaatgctc ttgctactag cacacgttgt
     2281 aattgtccac cacttaattc tgaaatttgt cgatgacgta aagattctaa ttctaaatcg
     2341 cttaataact gtttgagttt atcccttgct gatttattag gtcgtctaaa ccatccaatt
     2401 tctttgtagc aacctgataa aatcacttgt tccacactta taggaaaatc taaatcaata
     2461 tgtgcttttt gtggaatata tgtaatatgt tgcagttgtt gttgtatagg tttgttatat
     2521 aacaatttag taccggtagc attaaattca ccaattaaag acttgataag ggaagattta
     2581 ccagcaccat tcgggcccat gataccaatt atttcgccgc gtactggtat cgataaggaa
     2641 atgtttttaa gtacatgctt attacctaaa aacagattta aatcttttgt ttctaacaaa
     2701 cgtttatacc tcctaattaa aagtttaggc taacctaatt aattgtataa taaactgaga
     2761 atatttatca tgtcaagtaa attcgtgata taatatagac aatgtatgtg aggtgaaagt
     2821 atgttaactg aagaaaaaga ggactattta aaggcaatcc ttacgaataa tggcgataaa
     2881 aactttgtga caaataaaat cttatctcaa tttttaaata ttaagcctcc atctgtaagt
     2941 gaaatggtag gacgtcttga aaaagcaggc tatgttgaaa caaaaccata caaaggtgtt
     3001 agattaacag aggatggttt aacgcatacg cttgatatca ttaagagaca tcgactatta
     3061 gaattatttt taatagaaat attgaaatat aattgggaag aagtacatca agaagcagaa
     3121 attttagaac atcgaatttc agatttattt gttgaaaggc tggatagcct gttaaatttc
     3181 ccagaaactt gcccgcacgg cggtgtgatt cctagaaata atgaatataa agagaaatat
     3241 ataacaacga ttttgaatta tgaacctggt gatatcgtta caatcaaacg tgtgagagat
     3301 aagaccgatt tgctaatata tttgtctagt aaagatattt ctattggtaa tgaagtggaa
     3361 attgtatcga aagatgaaat gaataaagta attatcatta aacgtaatga taatgtaatt
     3421 attgtcagtt acgaaaatgc aatgaacatg tttgctgaaa aataaaataa agaagccata
     3481 aagatatcca tgattgaact gataaagaca tatggataat tgctttaggc ttctttttta
     3541 ttagttaatt tatcaagtga gtatatttga gtaaaatatt cactgcataa agattgaaga
     3601 taatccagat tgtactataa atgaagatag gtacatgact gagttcttta agtgcactac
     3661 catcccactg tggactcgga cgctggaaag tcaatttagc aatcgtccaa ctagattgta
     3721 gaacttcgcc taataataca cctaaaatat attgataact cattgtgaca agtagttgaa
     3781 tttctactat attttcatct tttaatataa aatacaacat gatagaaatt aaagttataa
     3841 caacaatggg tgagcctttt ctagatgtta aaattaaaaa ataaataaat atcaataaat
     3901 aggtaaatat aaagaaacta ggtatctgat aatggctcga cgctaaacct atcaataaca
     3961 taataggtgg cataaaataa ccaccaatcg ttgtaagcca ttggcctgct agatgtctag
     4021 attgtgtaat tgcgaatcct tgttgtaatg tctgttgtcg ctctcgtgga cttgttacaa
     4081 tgactaaatc ttttgcacgg ccaccagcga gtttattaaa cagtacatga ccaaattcat
     4141 gtgttaaaac agggatatag tttaaaatga catctaaata gttcaaaaca ggcttatgtc
     4201 tatattgatg aatagcaata taacaagctg caacaataac gataatgtat atattaagtt
     4261 gaattgtcgt attaaaaaag tttgataaat aattcattgt taacctcata taagatatta
     4321 atttaaagtt tgcttatcac ttattataaa tgatattggc atcaatagcg ttagacttta
     4381 gacttacctt agttaaacta attttaattt ttgaaaaggt gaatatgtgt taaaataaag
     4441 caaaatcatt tcgatataaa taggatgaat ataaatactg ttaatattga ttacactaac
     4501 ataataatga aataagatag gagattcctg ttatgactgt tgaagaaaga tccaatacag
     4561 ccaaagttga cattttaggg gtcgattttg ataatacaac aatgttgcaa atggttgaaa
     4621 atattaaaac cttttttgca aatcaatcaa cgaataatct ttttatagta acagccaacc
     4681 ctgaaatagt gaattacgcg acgacacatc aagcgtattt agagttaata aatcaagcga
     4741 gctatattgt tgctgatggg acaggagtag tcaaagcttc gcatcgttta aagcaacctc
     4801 tagcgcatcg tatacctggt attgagttga tggatgaatg tttgaaaatt gctcatgtaa
     4861 atcatcaaaa agtatttttg ctaggggcaa ctaatgaagt tgtagaagcg gcacaatatg
     4921 cattgcaaca aagatatcca aacatatcgt ttgcacatca tcacggttat attgatttag
     4981 aagatgagac agtagtgaaa cgaattaaac tgtttaaacc tgattacata tttgtaggta
     5041 tgggattccc taaacaagaa gaatggatta tgacacatga aaaccaattt gaatctacag
     5101 tgatgatggg cgtaggtggt tctcttgaag tatttgctgg ggctaaaaag agagcgcctt
     5161 atatctttag aaaattaaac attgaatgga tatatagagc attaatagat tggaaacgta
     5221 ttggtagatt aaagagtatt ccaatattta tgtataaaat agccaaagca aaaagaaaaa
     5281 taaaaaaggc gaaataatca tgatgacaaa aataaaaccg aggaaatcct taaatggaga
     5341 ttctcggttt tttcggttta tttaataacg aagcgggact catcgagttt gtttctaaat
     5401 tctttttgtt cggctttgga tttcttttta aaatcgttaa ggaaagcttc atatttaggt
     5461 aatacatcat caagttcacc gtaatctttt aactttccgc cttcaatcca agcaatctta
     5521 gtacaaaatt gtctcacttg tcctaagtta tgactaacga aaaagatggt tttgttttgc
     5581 tctttaaact cgtaaatttt atctaaacat ttttgtgcaa aagtttggtc acctacagat
     5641 aaagcttcgt caatgactaa gatatctgga ttaactgtga tattaattga aaaaccaagt
     5701 tttgcacgca taccacttga atacttttta actggttgat aaataaactc accaagttca
     5761 ctaaattcaa taatcttagg tgtcatcgct ttaatttctt ttcgcttaaa gcccatacat
     5821 aacattttaa attcgatatt ttcaatccct gtaagttgtc cactcaagcc agcactaatt
     5881 gcgataacgc tgacttcacc attacgatcc actttgccaa cagtaggcga caaagaaccg
     5941 ccaatgatat tgctcaacgt tgatttgccg gaaccattga tgccaacaag ccctatgacg
     6001 tcaccttcat atgcttttaa actaatgtca tctaaagcga aaaatgtttt gtttttatgt
     6061 ttgggaatga gcgcatcttt catacgttct ttatttgtac gataaatacg atattctttt
     6121 gttacatttt taatgtttac cgaaacgttc atttgtagac cttccttatt cacatttatc
     6181 tagattataa tatactactc aacagttgtt aaattttaaa acctgttgta aagtgtatag
     6241 aagattttgt tattatcaga gtgggtgttt tgacacaaaa tgttaatcat caatgataac
     6301 aatgatattt aaaaactaaa cttatttcaa cttacatgat tgtatactat aatgtatttg
     6361 taataaacta atattttaaa gaactagaca ataattttga tagcatccat gtatagtgat
     6421 agtatttaca acaattatta taatactatt tagttaagta gagaaatagt taaacatttg
     6481 aaagtgtggt ttaatggaat gtcagcaata ggaacagttt ttaaagaaca tgtaaagaac
     6541 ttttatttaa ttcaaagact ggctcagttt caagttaaaa ttatcaatca tagtaactat
     6601 ttaggtgtgg cttgggaatt aattaaccct gttatgcaaa ttatggttta ctggatggtt
     6661 tttggattag gaataagaag taatgcacca attcatggtg taccttttgt ttattggtta
     6721 ttggttggta tcagtatgtg gttcttcatc aaccaaggta ttttagaagg tactaaagca
     6781 attacacaaa agtttaatca agtatcgaaa atgaacttcc cgttatcgat aataccgaca
     6841 tatattgtga caagtagatt ttatggacat ttaggcttac ttttacttgt gataattgca
     6901 tgtatgttta ctggtattta tccatcaata catatcattc aattattgat atatgtaccg
     6961 ttttgttttt tcttaactgc ctcggtgacg ttattaacat caacactcgg tgtgttagtt
     7021 agagatacac aaatgttaat gcaagcaata ttaagaatat tattttactt ttcaccaatt
     7081 ttgtggctac caaagaacca tggtatcagt ggtttaattc atgaaatgat gaaatataat
     7141 ccagtttact ttattgctga atcataccgt gcagcaattt tatatcacga atggtatttc
     7201 atggatcatt ggaaattaat gttatacaat ttcggtattg ttgccatttt ctttgcaatt
     7261 ggtgcgtact tacacatgaa atatagagat caatttgcag acttcttgta atatatttat
     7321 atgacgaaac cccgctaacc attaataaat ggaagtgggg ttcatttttg tttataattt
     7381 aagtaaataa catattaagt tggtgtatta tgaacgtttt aataaagaaa ttttatcatt
     7441 tggtagttcg aatactttct aaaatgatta cgcctcaagt gattgataaa ccgcatatcg
     7501 tatttatgat gacttttcca gaagatatta agcctatcat caaagcatta aataattcgt
     7561 cgtatcagaa aactgtttta acaacaccaa aacaagcgcc ttatttatct gaacttagcg
     7621 acgatgttga tgtgatagaa atgactaatc gaacattggt aaaacaaatt aaggctttga
     7681 aaagcgcgca gatgattatt atcgataatt attacctatt actaggtgga tataataaga
     7741 cttctaatca acacattgtt caaacgtggc atgcaagtgg tgcattaaaa aactttggct
     7801 taacagatca tcaagtcgat gtgtctgaca aggcaatggt tcagcagtac cgtaaagttt
     7861 atcaagcgac ggatttttac ttagtgggtt gtgaacaaat gtcacaatgt tttaaacagt
     7921 ctttaggtgc aacagaagag caaatgctgt attttgggct tccgagaatt aataaatatt
     7981 acacagctga tagagcaacg gttaaggcag agttaaagga taaatatgga attacaaata
     8041 agttggtatt atatgtacca acatatagag aagataaagc agataatagg gctattgata
     8101 aagcttattt tgaaaaatgt ttaccaggat atacactgat taataaatta catccatcaa
     8161 ttgaagattc agacattgat gacgtatctt caatcgacac gtctacatta atgctaatgt
     8221 cagatataat tattagcgac tatagttcgc tgccaataga agctagcttg ttagatattc
     8281 caactatatt ttatgtgtat gatgaaggaa catatgatca ggtgagaggc ctgaatcaat
     8341 tttacaaagc aataccggat agctacaaag tgtatactga agaagattta ataatgacga
     8401 tacaagaaaa agaacatcta ttaagtccgt tatttaaaga ttggcataag tataatactg
     8461 ataaaagttt acatcagctc acagaatata tagataagat ggtgacaaaa tgaggtttac
     8521 gataatcata cctacatgta ataatgaggc aacaattcga caattgttaa tatctattga
     8581 gagtaaagaa cactatagaa tcctttgtat tgatggtggt tctactgatc aaataattcc
     8641 tatgattgaa cggttacaaa gagaactcaa gcatatttca ttaatacaat tacaaaatgc
     8701 ttcgatagct acgtgtatta ataaaggttt gatggatatc aaaatgacag atccacatga
     8761 tagtgacgca tttatggtca taaaaccaac atcaatcgta ttgccaggta aattagatag
     8821 gttaactgct gctttcaaaa ataatgataa tattgatatg gtaatagggc agcgagctta
     8881 caattaccat ggtgaatgga aattgaaaag tgctgatgag tttattaaag acaatcgaat
     8941 cgttacatta acggaacaac cagatttgtt atcaatgatg tcttttgacg gaaagttatt
     9001 cagtgctaaa tttgctgaat tacagtgtga cgaaacttta gctaacacat acaatcacgc
     9061 aatacttgtc aaggcgatgc aaaaagctac ggatatacat ttagtttcac agatgattgt
     9121 cggagataac gatatagata cacatgctac aagtaacgaa gaagatttta atagatatat
     9181 cacagaaatt atgaaaataa gacaacgagt catggaaatg ttactattac ctgaacaaag
     9241 gctattatat agtgatatgg ttgatcgtat tttattcaat aattcattaa aatattatat
     9301 gaacgaacac ccagcagtaa cgcacacgac aattcaactc gtaaaagact atattatgtc
     9361 tatgcagcat tctgattatg tatcgcaaaa catgtttgac attataaata cagttgaatt
     9421 tattggtgag aattgggata gagaaatata cgaattgtgg cgacaaacat taattcaagt
     9481 gggcattaat aggccgactt ataaaaaatt cttgatacaa cttaaaggga gaaagtttgc
     9541 acatcgaaca aaatcaatgt taaaacgata acgtgtacat tgatgaccat aaactgcaat
     9601 cctatgatgt gacaatatga ggaggataac ttaatgaaac gtgtaataac atatggcaca
     9661 tatgacttac ttcactatgg tcatatcgaa ttgcttcgtc gtgcaagaga gatgggcgat
     9721 tatttaatag tagcattatc aacagatgaa tttaatcaaa ttaaacataa aaaatcttat
     9781 tatgattatg aacaacgaaa aatgatgctt gaatcaatac gctatgtcga tttagtcatt
     9841 ccagaaaagg gctggggaca aaaagaagac gatgtcgaaa aatttgatgt agatgttttt
     9901 gttatgggac atgactggga aggtgaattc gacttcttaa aggataaatg tgaagtcatt
     9961 tatttaaaac gtacagaagg catttcgacg actaaaatca aacaagaatt atatggtaaa
    10021 gatgctaaat aaattatata gaactatcga tactaaacga taaattaact taggttatta
    10081 taaaataaat ataaaacgga caagtttcgc agctttataa tgtgcaactt gtccgttttt
    10141 agtatgtttt attttctttt tctaaataaa cgattgatta tcatatgaac aataagtgct
    10201 aatccagcga caaggcatgt accaccaatg atagtgaata atggatgttc ttcccacata
    10261 cttttagcaa cagtatttgc cttttgaata attggctgat gaacttctac agttggaggt
    10321 ccataatctt tattaataaa ttctcttgga tagtccgcgt gtactttacc atcttcgact
    10381 acaagtttat aatctttttt actaaaatca cttggtaaaa catcgtaaag atcattttca
    10441 acataatatt tcttaccatt tatcctttgc tcacctttag acaatatttt tacatattta
    10501 tactgatcaa atgagcgttc cattaatgca ttccccatca tattacgttg cttctcgcca
    10561 ccaaggtttt tatagtctcc tgcacccatg ataacttgat taattctaaa tttacctcgt
    10621 ttggtagtaa tcgtatggtt gtaatttgct gtatcacttg atccagtttt taaaccatct
    10681 gtacccggca aactcatttt tgcaccttcc aatgaaaagt tgaatgtgta atacgtaact
    10741 gcatgcgttg ttggtgctaa ctgctttgta aagtctaata ttttaggtgt ctctttaatc
    10801 acgtgtaaat ctaaaatggc atagtctcta gcagtcgtta cagtacgttc ttggtcttta
    10861 tactttgttg gtgcaaatgt acgtaatctt gaattttcag cacccgttgg attgacgaaa
    10921 tgtgtatttt tcattccgat agctttagct ttgttattca ttaaatcaac gaaatcgctg
    10981 gtgttttttg aaaccttctt agctaaaatt aatgccgcgg cattactaga attagatact
    11041 gtaatttgta ataggtctgc gattgtccat acttgtccag gatatagttt cgtattactc
    11101 aactcaggta gtgtagacat aatatattct ttgttcgtca ttgtgactgt gtcatcaagt
    11161 gaaagctgcc ccttatttac agcttccaat gttaagtaca ttgtcattaa tttagtcata
    11221 gacgctggat tccacttagt atcgatattg tattgataca gtaattgtcc agtttgactt
    11281 acattaacag cactcgtcgg ttcgtatgca gccgacaaac ctgcataacc atattgattt
    11341 gctgcttgta caggggttac gtcactgtta gtagcttgtg catatggtgt cataatactt
    11401 aatgttaaac ataaaatgat gataatagat attaaatttt tcataaagcg ttaatcttcc
    11461 cttttccaat tcttaaatat tccctaaaag caatggttat tcctacttac ggaaatcatt
    11521 gctaattcac ttcaccttaa ttaaattgtt gaaaataaag ttttctgcag ttaatttgaa
    11581 aaataatgca aatatattac gtgtgtagct aaaggtgtta taatgtttgt acgaagagca
    11641 aacttactca aaagcgatta attttcatgt tttaatataa agactttgag aagttattac
    11701 aaaaaatgca atagaaatat tctatcatat aaatgttatg agcggtattt tggggcaaca
    11761 ctttatttga tttttaaagt tttgttggga gaaagtatat gatagaaatg catgtatcta
    11821 tctaaatgaa ttaactataa atttcaaaca gaagaggtaa aactatgaaa cgagaaaatc
    11881 cattgttttt cttatttaaa aaactatcat ggccagtggg tcttatcgtt gcagctatca
    11941 ctatttcatc actagggagc ttaagtggac tattagtgcc actgtttact ggacgaattg
    12001 tagataaatt ttccgtgagc catatcaatt ggaatctaat cgcattattt ggtggtatct
    12061 ttgtcatcaa tgctttatta agcggattag gtttatattt attaagtaaa attggtgaaa
    12121 agattattta tgcgatacgc tcagttttat gggagcatat catacaatta aaaatgccat
    12181 tctttgacaa aaatgaaagt ggtcaattaa tgagtcgatt aactgacgat acgaaagtga
    12241 taaatgaatt tatttcacaa aagctaccta acttattacc atcaatcgtt acattagttg
    12301 ggtcactaat catgttattt attttagatt ggaaaatgac attattaaca tttataacga
    12361 taccgatatt cgttttaatt atgattcctc taggtcgtat tatgcaaaag atatcgacaa
    12421 gtacacaatc tgaaattgca aacttcagtg gtttgttagg gcgtgtccta actgaaatgc
    12481 gtcttgttaa aatatcaaat acagagcgtc ttgaattaga taatgcacat aaaaatttga
    12541 atgaaatata taaattaggt ttaaaacagg ctaaaattgc ggcagttgta caaccaattt
    12601 caggtatagt tatgttgcta acaattgcaa ttattttagg ttttggtgca ttagaaattg
    12661 cgactggtgc aatcactgca ggtacattaa ttgcaatgat attttatgtt attcagttat
    12721 ctatgccttt aatcaatctt tccacgttag ttacagatta taaaaaggca gtcggtgcaa
    12781 gtagtagaat atacgaaatc atgcaagaac ctattgaacc gacagaagct cttgaagatt
    12841 ctgaaaatgt attaattgat gacggtgtat tgtcatttga acatgtagac tttaaatatg
    12901 atgtgaagaa aatattagat gatgtgtcgt tccaaatccc acaaggtcaa gtgagtgctt
    12961 ttgtaggccc ttctgggtct ggtaaaagta cgatatttaa tctgatagaa cgtatgtatg
    13021 aaattgagtc aggtgatatt aaatatggcc ttgaaagtgt ctatgatatc ccgttatcta
    13081 agtggcgacg caaaattgga tatgttatgc aatcaaattc gatgatgagt ggtacaatta
    13141 gagacaatat tttatacgga attaatcgtc atgtttcaga tgaagaactt attaattatg
    13201 ctaaattagc gaactgtcat gattttatca tgcaatttga tgaaggatat gacacgcttg
    13261 taggtgaacg aggattgaaa ctgtctggcg gacaacgtca acgtattgat attgctagaa
    13321 gttttgttaa aaatcctgat attttgttac ttgatgaagc aacagctaat ctcgatagtg
    13381 aaagtgaatt gaaaattcaa gaagctttag aaacattgat ggaaggtaga acaacgattg
    13441 tcattgcgca tcgtttgtct acaattaaaa aagccggtca aattatattc ttagacaaag
    13501 gacaggtaac aggtaaaggt acgcattcag aactgatggc atcacatgcg aagtataaaa
    13561 actttgtagt gtctcaaaaa ttaacagatt aattttatat atataagtaa gcttggagca
    13621 aatacacata taccatcgag gaaattaaag tgtggcacat tgatggatat agatgttaat
    13681 aaattgcttc aagcttttgt ctattttaaa tcatttgaga agttacgaca taataattct
    13741 taaattaatg aaatcgatat tttaagaaaa aaatgctcat ggtataatac aagttataag
    13801 caaacataca tatattaaat actgtagcca cgagtcataa ttcttcgtat tttacatagc
    13861 aatttaactg attttagagt ccacggtaca gaagtttgat atttcaatgt ttctaaattt
    13921 ttaaaaaatt aaatcatagg tgggtgccaa atgtttttat taatcaacat tattggtcta
    13981 attgtatttc ttggtattgc ggtattattt tcaagagatc gcaaaaatat ccaatggcaa
    14041 tcaattggga tcttagttgt tttaaacctg tttttagcat ggttctttat ttattttgat
    14101 tggggtcaaa aagcagtaag aggagcagcc aatggtatcg cttgggtagt tcagtcagcg
    14161 catgctggta caggttttgc atttgcaagt ttgacaaatg ttaaaatgat ggatatggct
    14221 gttgcagcct tattcccaat attattaata gtgccattat ttgatatctt aatgtacttt
    14281 aatattttac cgaaaattat tggaggtatt ggttggttac tagctaaagt aacaagacaa
    14341 cctaaattcg agtcattctt tgggatagaa atgatgttct taggaaatac tgaagcatta
    14401 gccgtatcaa gtgagcaact aaaacgtatg aatgaaatgc gtgtattaac aatcgcaatg
    14461 atgtcaatga gctctgtatc gggagctatt gtaggtgcgt atgtacaaat ggtaccagga
    14521 gaactggtac taacggcaat tccactaaat atcgttaacg cgattattgt gtcatgcttg
    14581 ttgaatccag taagtgttga agagaaagaa gatattattt acagtcttaa aaacaatgaa
    14641 gttgaacgtc aaccattctt ctcattcctt ggagattctg tattagcagc aggtaaatta
    14701 gtattaatca tcatcgcatt tgttattagt tttgtagcgt tagctgatct atttgatcgt
    14761 tttatcaatt tgattacagg attgatagca ggatggatag gcataaaagg tagtttcggt
    14821 ttaaaccaaa ttttaggtgt gtttatgtat ccatttgcgc tattactcgg tttaccttat
    14881 gatgaagcgt ggttggtagc acaacaaatg gctaagaaaa ttgttacaaa tgaatttgtt
    14941 gttatgggtg aaatttctaa agatattgca tcttatacac cacaccatcg tgcggttatt
    15001 acaacattct taatttcatt tgcaaacttc tcaacgattg gtatgattat cggtacattg
    15061 aaaggcattg ttgataaaaa gacatcagac tttgtatcta aatatgtacc tatgatgcta
    15121 ttatcaggta tcctagtttc attattaaca gcagctttcg ttggtttatt tgcatggtaa
    15181 tatgtcgaag agtgactatg ataatacatt ttaactaata aatatgtcca ggcatgtcgt
    15241 ctattgatat aggtgagatg cttggacttt tttattattg atataaaggt atttaaatat
    15301 ttttaaagtt accgaaattg aagcattata aaaaccaagt gcacatggta atacacttgg
    15361 cttttatggg aaatgaatat tattgtacat atgacagtaa ggactaggta cagtcatagt
    15421 acttcgagca aaatttgttt tgttattata aacaacacaa aggagataac ttctctattg
    15481 aagaagttaa aaacattata gcagacaatg aaatgaaagt aaattaaaaa ttcagaatat
    15541 ttttaattat tatattgtga gtgatattta ttagggaaag ctattcttca tataaattag
    15601 ttaaatagta aatctttgtt agaactcttt ccatagagtt gtgacagcat ttttgttgtg
    15661 ctactatttt tatagtctaa taaatatata aaggggatgg tttcgtgaat aaaacggtta
    15721 aagatttaat actagttgtc ttaggttcat ttatctttgc tgcaggtgta aatgcattta
    15781 ttatttctgg taacttaggt gaaggcgggg ttacaggttt agcaattatt ttatattatg
    15841 cgtttcatat ttcaccagcc atcactaact tcttggtcaa cgcagtattg attgccatag
    15901 gttataaatt tttgagtaag agaagtatgt acttaactat tcttgtaaca attcttattt
    15961 caatattttt gagtttaaca gaatcatggc aagtagaaac tggaaacagc attgtgaatg
    16021 ccatttttgg tggtgtaagc gttggactag gaatcggagt aattatcctt gcaggcggta
    16081 caacagcagg tacaacaatt ttggcgagaa ttgcaacgaa atacctcgat gtaagcacgc
    16141 catatgcttt gcttttcttc gatatgatcg ttgttgcaat ttcacttaca gttattccac
    16201 ttgataaagt attagtaaca gtaatatcac tttatatagg aacaaaagtg atggaatatg
    16261 tcatagaagg tttaaacact aaaaaagcta tgacgattat ttcaactaat cccgacaaac
    16321 ttgccaaagc aatagacgag caaattggaa gaggtttaac cattttaaac ggacatggct
    16381 attatacgcg tgaagaaaaa gatgtcttat acgttgttat ttctaaaaca caagtttcaa
    16441 aagcaaagcg attaattaaa caaatcgata aagatgcatt cctcgtaatt catgatgtaa
    16501 gagatgtcta tggtaatggc tttcttgcag atgaataaat aaatggtatg agcacacata
    16561 cttaaataga agtccacgga caagtttttg aactatgaag acttatctgt gggcgttttt
    16621 tattttataa aagtaatata caagacatga caaatcgagc tatccaattt aaaaagtaat
    16681 gttagtcaat aagattgaaa aatgttataa tgatgttcat gataatcatt atcaattggg
    16741 atgtctttga aaattgataa tttaaaaata gaaattattt tttataaaca gaaagaattt
    16801 tattgaaagt agggaaatta tgaatcgttt gcatggacaa caagttaaaa ttggttacgg
    16861 ggataacacg attataaata aattagatgt tgaaatacca gatggcaaag tgacgtcaat
    16921 cattggtcct aacggctgcg ggaaatctac tttgctaaag gcattgtcac gtttattggc
    16981 agttaaagaa ggcgaagtat ttttagatgg tgaaaatatt catacacaat ctacgaaaga
    17041 gattgcaaaa aaaatagcca ttttacctca atcacctgaa gtagcagatg gcttaactgt
    17101 tggggaatta gtttcatatg gtcgttttcc acatcaaaaa ggatttggta gattaactgc
    17161 tgaggataag aaagaaattg attgggcaat ggaagttaca ggaactgata cattccgaca
    17221 ccgttcaatc aatgatttaa gtggtggtca aagacaacgt gtttggattg caatggcatt
    17281 agcacaaaga actgatatta tctttttaga cgaaccaaca acatatttag atatctgtca
    17341 tcaattagaa atactagaat tagttcagaa gctaaatcag gaacaaggtt gtacaattgt
    17401 catggttctt catgatatca accaagcgat tcgtttctca gatcatctta ttgcgatgaa
    17461 agaaggggat atcatcgcta caggttcaac agaagacgta ttaacacagg aaatattaga
    17521 aaaagttttt aatattgatg ttgttttaag taaagatcct aaaactggaa aacctttact
    17581 ggtaacttat gacttatgtc gcagagctta ttcttaatta agtaagttaa tatgataaaa
    17641 aggacaatta acatgacaaa tagagagaac ccaacgccat tgaagttttt atcctatatt
    17701 ataggtttaa gtatgatact actaatcaca ctatttattt ctacattaat aggtgacgcc
    17761 aaaattcaag cctctacaat tatagaggct atttttaatt ataatcctag caatcaacag
    17821 caaaacatca tcaatgagat taggattccc agaaatatag cagcagtaat tgtaggtatg
    17881 gcgcttgcag tttctggtgc gattatacaa ggtgttactc gtaatggtct tgctgatccg
    17941 gcgctcatag gtttaaattc aggtgcttca tttgctttag cattaacata tgcagtttta
    18001 ccaaacactt catttttaat attgatgttt gctggatttt taggtgctat tctaggaggt
    18061 gctattgtat taatgatagg ccgatctaga cgtgatggat ttaatccgat gcgtattatt
    18121 ttagcgggtg cagcagtaag tgctatgtta acagcgctaa gtcaaggtat tgcattagct
    18181 tttagactaa atcaaacagt aacattttgg actgctggag gcgtttcagg cacaacatgg
    18241 tcacacctta agtgggcaat tccattaatt ggtattgcgt tattcattat attaacaatt
    18301 agtaaacaac ttaccatttt aaatcttggt gaatcattag ctaaaggttt aggtcaaaat
    18361 gtaacaatga tcagaggcat atgtttaatt attgctatga ttctagcagg tattgcagtt
    18421 gctatcgctg gacaagttgc atttgtaggt ttgatggtac ctcatatagc aagattttta
    18481 attggaactg attatgctaa aattctacca ttaacagcct tgttaggtgg gatactcgtg
    18541 cttgttgccg atgtgatagc acgatattta ggagaagcgc ctgttggtgc aatcatttca
    18601 tttatcggtg ttccttactt tttatattta gttaaaaaag gaggacgctc aatatgatta
    18661 gttcaaataa taaacgcaga caattgatag cactggctgt ttttagcatt ctactatttc
    18721 taggttgtac ttggagtatt acctcaggtg aatacaacat acctgttgaa agatttttca
    18781 aaactttaat tggacaaggt gatgccattg ataagttaat cttattagat ttcaggttac
    18841 ctcggatgat gattactatt ttggctggcg cagcgcttag tattagtggt gcaatagtgc
    18901 aaagtgtcac aaaaaatcca atagctgaac caggtatatt aggtattaac gcaggtggcg
    18961 gatttgcaat cgcattattt attgcaattg gtaaaattaa tgctgacaac tttgtttatg
    19021 tactgccgtt aataagtata ctaggtggta tcaccactgc attgattatt tttattttca
    19081 gttttaataa aaatgaaggt gttacacctg cgagtatggt attaataggt gtaggtttac
    19141 aaacagcatt atatggtggc tcaattacaa ttatgtcaaa atttgatgat aagcaatctg
    19201 atttcatcgc tgcttggttt gcaggtaata tttggggtga cgaatggcca tttgtcattg
    19261 catttttacc gtgggtgttg attattattc cttacttact atttaaatcg aatacactaa
    19321 atattattca tacgggtgat aatattgcac gaggtctagg tgtaaggtta agcagagaac
    19381 gtttaatatt attctttatc gcagtgatgt tatcatctgc tgctgtagca gtagcaggtt
    19441 caatttcgtt tatcggatta atgggtccgc atattgccaa acgtatcgtt ggaccacgtc
    19501 accagttgtt tttaccaatt gccattttag taggggcatg tttacttgtt atagctgata
    19561 caattggcaa aattgtatta caaccaggtg gggttccagc aggtattgtc gtagcaatta
    19621 ttggtgcacc gtatttctta tatttaatgt acaaaacgaa aaatgtatag tgtcaatgga
    19681 cacaacttat tgctatgaaa ggcactttat tataaggctt ttcatagcat tttttattta
    19741 atgagccact caagactatt tattttttca ataatgaacc attaagttat caagaggatc
    19801 ttatcaaaaa tatatttgat aacggtatca ggttaattct ttatgatagc gcattcattt
    19861 attctgtttt atactatgac tgataatacc aaggaggtac aacatgatga aaaagttaat
    19921 caataaaaaa gaaacatttt taactgatat gcttgaagga ttgttaattg cgcacccaga
    19981 gttagatctg attgctaata cagttattgt aaaaaaagct aagaaagaac atggtgtagc
    20041 aatagtctct ggaggtggaa gcggacatga acctgcgcat gccggttttg ttgcagaagg
    20101 tatgctagat gcagcggttt gtggcgaagt atttacatca cctacacctg ataaaatatt
    20161 agaagctatt aaagcagtag atactggtga tggtgtatta ctagttgtaa aaaactatgc
    20221 aggtgacgtg atgaatttcg aaatggcaca agagcttgca gaaatggaag gtataaatgt
    20281 tcaaactgtt attgttcgtg acgacattgc tgtgacaaac gaagtacaac gtcgtggtgt
    20341 tgcaggaaca gtgtttgttc ataagcttgc cggttatctt gctgaaaaag gttattcatt
    20401 aacagagata aaatcgcgtg tagaagcgtt gttacctgaa attaaaagta ttggtatggc
    20461 aattgagcca ccgcttgttc caactactgg aaaatatggc tttgatattg aagacgacaa
    20521 aatggaaatc ggtattggta tacatggtga aaaaggtatt catagggaag aagtaaagga
    20581 tattgatcat attgttggaa cattgttaga cgaattgtat aaagaagtta ctgccaatga
    20641 tgtcatatta atggtaaatg gtatgggtgg tacgccgtta tctgaattaa atatcgtaac
    20701 taaatatatt caacaaaatt tagctgcaag aacggttaat gttgctaaat ggtttgttgg
    20761 tgattatatg acatctttag acatgcaagg tttttctata actatcgtgc ctaataaacc
    20821 agaatatttg gaagcatttt tagcaccaac aacaagtcaa tactttaaat aagaagtgaa
    20881 tatgaataaa tatacattta tgaggtggca caaatgaaag tgaatgatat gaaagcacgt
    20941 ttattaaatt tagaagaaac gtttaaaaaa catgaatctg aattaactga attagatcga
    21001 gcaattggtg atggtgacca cggggttaac atggttcgtg ggtttagtag tcttaaagac
    21061 aaacttgatg atagctcaat gcaatcattg ttcaaatcaa ctggtatggc attgatgtca
    21121 aatgttgggg gtgcatcagg accactgtat ggctttagct ttgttaaaat gtctgcagtc
    21181 accaaagatg atatggataa tcaagatttc attacactaa ttcaggcatt tgccgaagcg
    21241 gttgaatcac gtggtaaagt tactttaaat gaaaagacaa tgtatgatgt agtagcgcga
    21301 gcagcagaga agcttaaaaa tggtgaaact ttaacattca atgatttaca gcaattagca
    21361 gataatacaa aagatatggt agcaacgaaa ggtagagctg catattttgg agaagaatca
    21421 aaaggttata ttgatccagg tgctcaaagt atggtttata ttttaaacgc tttgattgga
    21481 gatgaagata atgcctaaaa ttatacttgt tagccacagt aaagaaattg caagtggtac
    21541 aaaatctttg ttaaagcaaa tggcaggtga cgttgatatt ataccaatcg ggggattacc
    21601 agatggttca attggaactt catttgatat catccaagaa gttttgacta aattagagga
    21661 tgatgcattg tgtttttacg atattggatc ttcagaaatg aatgtagata tggcaattga
    21721 aatgtatgat ggtaatcatc gtgtgttaaa agttgatgca ccaattgttg aaggcagttt
    21781 tatcgcagca gtaaagctat caatcggcgg ttcaattgat gatgcattag cagaaatcaa
    21841 acaatcattt tagttaaaat ttactaataa tgaaaaatgt aaaccttttt caaatgaaac
    21901 tttataaaaa atatgatagt atatatgtaa atgtttaata aaatctggag aaataggagg
    21961 acattgccat gcaacacctt ataaaaaaac atgtattgaa tggcgagttt gatttagtac
    22021 gacaattgat gtccgaaaca gattttatgg aatttgaaga agcatatatt tcaagtgcgc
    22081 atgaagtaga aagtatgatg ttttatacat gtattttaga tatgattaag tacgaagaat
    22141 catctgaaat gcatgactta gcatttttat tgcttgtgta tccactaagt gaatatgaag
    22201 gtgctttgga ttctgcttat tatcatgcag acgcttccat aaaacttact gacggcaaag
    22261 aagttaaaag tttgttacaa atgttattat tgcatgcgat accaacacct gttatttcag
    22321 ataagaaggc ttttgatatc gccaagcaaa ttttaaaatt agatcctaat aataatgttg
    22381 ctcgtaacgt cttaaaagac actgccaaac gtatggacaa cgttgttgtt gatataaatg
    22441 aattacacca acgtaatgca cgttaattac atttcaatta tattagctta ataatagttt
    22501 taacatttgg ttgggttggg catatgttcc agcctttttt aatacttaaa aactaacgaa
    22561 gtatacttgt gtgcacaaat ggtttttata caacatttta taaatttata cattttaata
    22621 aagaacatac gatagatggt ttaaaccttg ttaactgaga aattttgata tgtattcttc
    22681 gaaatttaac taaatatacg aaattcaaga agcacaataa ttaatcattt ttcctataca
    22741 aaagttcgta tgactgcatt ataaaagcat aaatttataa tttttttaaa tgtcattgaa
    22801 cgtgataatg tgaatggatt gagcaatttt gaaaaagtga aaaataacct atgcggcttg
    22861 caattaattt tcagtacgtt ataatgcaca ctgtgcaaaa ttaaggaggt ctattattca
    22921 catgatgatg aataaagaag caacaaaaat tggatttgcc tacgtcggca ttgtagtggg
    22981 cgcaggattt tcaactggac aagaagttat gcaatttttc actaaatatg gcttgtgggc
    23041 ttatttaggt gttattatat ctggttttat tttagctttt attgggcgcc aagtagcaaa
    23101 aattggtact gcctttgaag cgacaaatca tgaatcaaca ttacaatacg tattcggtga
    23161 aaagtttagt aaagtctttg attatatttt aatcttcttc ttatttggta tagctgtaac
    23221 catgatagct ggtgcaggcg caacatttga agaaagttat aacataccta catggctagg
    23281 tgctttaatt atgacattag cgatttatat tacgttgcta ttagacttta ataaaatagt
    23341 acgtgcacta ggtatcgtta caccattttt aattgtttta gttgtattaa tcgctggcgt
    23401 ttatttattt aaaggtcatg tttcattagc agaagttaac caagtagtgc ctgaagcaag
    23461 tatttggaag ggaatctggt ttggtacaat atatggtgga ttagcttttt ctgtaggttt
    23521 tagtaccatc gtagcaatcg gtggggatac tgaaaagcgt acagtgtcag gtgcaggcgc
    23581 gatgtatggt ggtattatct atactgtatt actagcattg atcaactttg cattgcaaag
    23641 tgaatatcca actattaaaa atgcctcaat tcctacattg acgttagcaa ataatatcca
    23701 tcctttaata gcaacagtgt tatctgttat tatgctggcg gttatgtata atactattct
    23761 aggactaatg tattcatttg cagcacgttt tacagaacca tacagtaaaa attatcatat
    23821 ctttattatt ataatgatgg tagcaggtta tttattaagt ttcgtaggat ttgctgaatt
    23881 aattaataag ttatatacaa ttatgggata tgtaggctta tttattgtag tagctgtaat
    23941 tattaaatat ttcaaacgta aaaatgcgga taaaaaacat attgcttaat atcatatgag
    24001 ggatatccga aactttacaa ttgaatcact ttgttttaac cttaaaagca attcgtctct
    24061 actcttatcg ggcgaattgc tttttatatt tattcagtct attaatatga gcgtctaaca
    24121 aatagagagg tacgatgtaa tgaataaaga taataaatgg acgatgataa ctgcgctttt
    24181 tataactgta atcagtgtat tgttagcatt tcatctgaaa caacattatg accaaattac
    24241 aaatgagaac catgctaata aagacaaaat taatattaaa aataaaaatg tgcgcattta
    24301 tcaaaacctt acatacaata gagttttccc taacagtaaa ttagatatta ttacacctgt
    24361 tgatatgtct tctaatgcca aactgccagt tattttttgg atgcacggtg gtggttatat
    24421 tgcgggtgat aagcagtata aaaacccatt attagcgaaa attgctgaac aagggtacat
    24481 tgttgtgaat gtaaattatg cattggcgcc acaatataaa tatcccacac cattaattca
    24541 aatgaatcaa gcaactcaat tcattaaaga aaataaaatg aatttaccta ttgattttaa
    24601 tcaagtaatt attggcggtg attctgcagg tgctcaatta gctagccaat ttacggcaat
    24661 acagacgaat gatcgcttaa gagaagccat gaaatttgat cagtcattca aaccatcgca
    24721 aattaaaggt gctatactat ttggtggttt ttataatatg caaacagtta gagaaactga
    24781 gtttccaaga atacagttat ttatgaaaag ttatactggc gaagaagatt gggaaaagag
    24841 ttttaaaaac atttcacaaa tgtcgacagt aaaacaatcg acaaaaaatt atccaccaac
    24901 atttttatct gttggagata gcgatccatt cgaaagtcaa aatatagaat tcagtaagaa
    24961 attacaagaa ttgaatgtac cagtagatac tttgttttat gatggtacgc atcatttaca
    25021 tcatcagtat caatttcacc ttaataaacc tgaatcgata gataatatca aaaaagtgtt
    25081 acttttctta agtcgtaata catcctctag tggtattcaa actgaagaga aaccacaaat
    25141 agaaaatccg agtaatgaat taccgttaaa tcctttaaac taatgataaa cagtagtaat
    25201 ttattgctta agcaacattt aagattttca aattaaaaac gaagaattta aaacatgtgg
    25261 tgctaatgtg taagagtctg agatataata aattgttagc ggttctttat catttctatc
    25321 tcactctatt atgatgtgac atttatttta caaactaatt tgttttgaac ctgaaaataa
    25381 ctttttacaa ctaaattgtc gaaaaacagt gtgtacatga tataataaat ttataaattg
    25441 aaaagaattc aaaagaaaaa ttaaaatata gattgagcac gaagtgattt gaaataaggt
    25501 tgtgaaaggg aatgacaagg tcagcattaa aaccatttaa aaataaacgc gttatggtta
    25561 ctggacgtat acaacgtgtt ttgtttaaaa attatttaga tagacatagc acatttaagc
    25621 cgaatgtaag gatattatta aaagatgtat ttgtttcagg tgtatcaata gatcatttat
    25681 ggttatatga gacaaataaa tactatgcat tggcaatgga acttattcat caacgagtaa
    25741 aatttagtgc gaatgttgta ccgtattaca aaataaatag aaataataat ttattcgtac
    25801 aagattatgg aattaagcgt aaaggtaggt taattactga agaagcttac aatcaaaaca
    25861 atcagtatca ggataagata tatgaaaaat taccggatat agattttaga ctcgaagatt
    25921 tttatagtaa ggaaaactaa acaacatata taaagcacca ctaatcgtag ttgatagtta
    25981 atcaattaca cattagtggt gttttatttt gaatttaaat ttgataaatg aataagagtg
    26041 tacgtattta tttgagatta gataacattg ataagtatga gtaatgtatt aggtaaccat
    26101 taaattcgtt gttatacgat tctgatacaa gattatgata aaatagcttt aattaagaat
    26161 tttagccatc atatagtcat cataatattt accatcgata aataacttat cttttaaaac
    26221 gccttcgatt tgaaaatcgg cacttttaaa aagctcgagg gcaggttggt tattgagtgg
    26281 tacatttgct tcaattcggt gtatttgatt gtttaaacac caagccataa tggcatcaag
    26341 aagtgcttgg ccaattccac gatgttgata taatttcttt acacctaaat caattttagc
    26401 aacatgttta atgcgttgaa atggtgtcgt attaacaaag gcaaagccaa cgagttgttc
    26461 atcactttca gcaacgaaga tgactttatg cggagaagtg atatattctt ctaattgttt
    26521 actagccgat gtgacgctag gatcatattc tcctggtgtg tagaacatat acggagattc
    26581 gtcgtatatg ttcgctaaca ttgaaatgaa attttctaca tctttgatac taactctacg
    26641 tataatatgg gccataaaaa gcctccagta ttttgaaatt ttaaaacatt tgctactatt
    26701 ataatatata tgtaactaaa aggtggagta atatgttttt tggttacagt ttaaaggagt
    26761 attttagatg aaacctaaag ttttattagc aggtggaaca ggatatattg gtaagtattt
    26821 aagtgaagtg attgaaaatg atgctgaact ttttgctata tcaaaatatc cagacaataa
    26881 aaaaacagat gatgttgaaa tgacttggat tcagtgtgat atatttcatt acgaacaggt
    26941 tgttgcagca atgaatcaaa tagatattgc tgtattcttt atcgacccaa caaagaattc
    27001 tgccaaaata acacaatcat cagcaagaga tttaacatta atcgcagcag ataattttgg
    27061 tcgagcagcg gctatcaatc aagtaaaaaa agtaatctac atacctggga gtcgttatga
    27121 taatgaaaca attgaacgcc taggtgcata tggtacacct gtagaaacaa caaatttagt
    27181 ttttaaacgt tctttagtta atgtagaatt acaagtttca aagtatgatg atgttagatc
    27241 aacgatgaag gtagttttac caaagggatg gacattaaag aacgttgtaa accattttat
    27301 tgcatggatg ggttacacta aaggaacttt tgtgaaaaca gaaaaatcac atgatcaatt
    27361 taagatatat attaagaata aggtgcgacc gctcgcagta tttaaaatag aagaaacagc
    27421 tgatggaata ataactttaa ttttattgag tggaagttta gtgaaaaaat atacagttaa
    27481 tcaagggaag ttagaattta gattaatcaa agagtcggca gtcgtttata tacatctata
    27541 cgattatatc cctcgattat tttggccgat ttattacttt atacaagcac caatgcaaaa
    27601 aatgatgatt catggctttg aagttgactg ccggattaaa gattttcaaa gtcgattaaa
    27661 atcaggagaa aatatgaaat atactaaatg atattgggtg atatggatgc aaatactact
    27721 agtagaagat gacaatactt tgtttcaaga attgaaaaaa gaattagaac aatgggattt
    27781 taatgttgct ggtattgaag atttcggcaa agtaatggat acatttgaaa gttttaatcc
    27841 tgaaattgtt atattggatg ttcaattacc taaatatgat gggttttatt ggtgcagaaa
    27901 aatgagagaa gtttccaacg taccaatatt atttttatca tctcgtgata atccaatgga
    27961 tcaagtgatg agtatggaac ttggcgcaga tgattatatg caaaaaccgt tctataccaa
    28021 tgtattaatt gctaaattac aagcgattta tcgtcgtgtc tatgagttta cagctgaaga
    28081 aaaacgtaca ttgacttggc aagatgctgt cgttgatcta tcaaaagata gtatacaaaa
    28141 aggtgatcag acgattttcc tgtccaaaac agaaatgatt atattagaaa ttcttattac
    28201 caaaaaaaat caaatcgttt cgagagatac aattatcact gcattatggg atgatgaagc
    28261 atttgttagt gataatacgt taacagtaaa tgtgaatcgt ttacgaaaaa aattatctga
    28321 aattagtatg gatagtgcaa tcgaaacaaa agtaggaaaa ggatatatgg ctcatgaata
    28381 atttgaaatg ggtagcttat tttttgaaat ctcgcatgaa ctggatattt tggatattgt
    28441 ttttaaactt ccttatgtta ggcattagtc taatcgatta tgattttcca atagacagtt
    28501 tattttatat tgtttctttg aatttaagtt taacaatgat ttttctttta ttgacatatt
    28561 ttaaagaagt aaaattatat aagcattttg acaaagataa agaaatagaa gaaattaaac
    28621 ataaagattt agcggaaacg ccatttcaac gtcatacagt tgattattta tatcgtcaaa
    28681 tctcagcgca caaagaaaag gttgttgagc aacagttgca attgaacatg catgaacaaa
    28741 ccattacaga atttgtgcac gacataaaaa cacctgtgac agctatgaaa ttattaattg
    28801 atcaagaaaa aaatcaagaa agaaaacaag cattactata tgaatggtct cgtataaact
    28861 cgatgctaga tacacagctg tatattacta gattagaatc tcaacgtaaa gatatgtatt
    28921 ttgattacgt gtcacttaaa cgcatggtca ttgatgaaat acaattaaca agacatatta
    28981 gtcaggttaa aggtattggt tttgatgttg actttaaagt ggatgattat gtttatacag
    29041 atataaaatg gtgtcgtatg attattagac aaattttgtc aaacgcattg aaatatagtg
    29101 agaattttaa tattgaaatt gggacagaat taaatgatca acatgtttcg ttatatatta
    29161 aagactatgg cagaggtatt agtaaaaaag atatgccgcg aatatttgaa cgaggattta
    29221 cgtcaacggc taacagaaat gaaacgacgt cttcaggtat gggtctatat ttagtaaata
    29281 gtgtaaagga tcaattaggt attcacctgc aagtcacgtc gactgttggt aaggggacaa
    29341 ctgtcagatt gattttccca ttacaaaatg aaattgttga acgcatgtcg gaagtgacaa
    29401 atttgtcatt ttaaacatgc gttttgttac ttagaattga tacatcaatg cagcttcaac
    29461 gttataataa gatagatgtt agtcatatgt taaattgaag atacaagtgc caaagcctaa
    29521 aggaaatgaa gttaagataa attataggag tgttaaagtg gcaattttag aagtaaaaca
    29581 attaacaaaa atatatggaa ctaaaaaaat ggcacaagaa gtgttgcgag atatcaatat
    29641 gtctattgaa gaaggcgagt ttattgctat tatgggtccc tctggatctg ggaaaacgac
    29701 attattaaat gttttaagtt caattgatta tatttcacaa ggttctatta cattaaaagg
    29761 aaaaaaatta gaaaagcttt caaacaagga attatctgat atacgcaagc atgatattgg
    29821 ttttattttt caagagtata atttactgca tacattgact gttaaagaaa acataatgtt
    29881 accactaacg gtacagaagt tagataaaga acatatgtta aatcgttatg aaaaagtagc
    29941 agaagcatta aatatattgg atattagtga taaatatccc tctgaattgt ctggtggaca
    30001 aaggcaacga acatcagctg ccagagcatt tataacattg ccttctatta tatttgctga
    30061 cgaaccaaca ggtgcattgg attctaaaag tactcaagat ttattaaaac gattaacaag
    30121 aatgaatgaa gcatttaagt ctacaattat tatggtaacg catgatcctg ttgcagcaag
    30181 ctatgcaaat cgagtagtga tgctaaaaga tggtcaaatt ttcactgaat tataccaagg
    30241 ggatgacgat aaacatacct ttttcaaaga aataatacgt gtacaaagtg ttttaggtgg
    30301 cgttaattat gacctttaac gagataatat ttaaaaattt ccgtcaaaat ttatcacatt
    30361 atgccatcta tcttttttcg ttaattacga gtgtagtatt gtattttagc tttgtagcat
    30421 taaaatacgc tcataaacta aacatgacag agtcatatcc aattataaag gaaggctcac
    30481 aagtcggaag ctactttcta tttttcatca taattgcatt tttgttatat gccaatgtgt
    30541 tatttattaa acgacgaagt tatgagcttg cattatatca aacattaggt ttatctaaat
    30601 tcaacattat ttatatacta atgctcgaac aattactaat atttataatt acggcaatat
    30661 taggtattat tattggtatt tttggttcga aactgttatt aatgattgtc tttacattat
    30721 taggaattaa agaaaaggtt ccaattattt ttagtttgag ggcggtattt gaaacattaa
    30781 tgttaatcgg tgtcgcttat tttttaacat ctgctcaaaa ttttatatta gtgttcaaac
    30841 aatctatttc acagatgtca aagaataacc aggttaaaga aacaaatcat aataaaatta
    30901 catttgaaga ggttgtttta ggcatcttag gtatagtatt gattaccaca ggatactatc
    30961 tatctttgaa cattgttcaa tattatgatt ctatcggtac acttatgttt attttattgt
    31021 caactgtgat tggggcatac ttatttttta aaagctctgt ttctctagtt tttaaaatgg
    31081 tgaagaagtt tagaaaaggt gttataagtg taaatgatgt catgttctca tcatctatta
    31141 tgtatcgtat taagaaaaat gctttttcac ttacggtcat ggcaatcatt tcagcgatta
    31201 ctgtttcagt tctttgcttt gctgctataa gtagagcgtc cttatcaagt gaaataaaat
    31261 atactgcacc acacgacgtt acaattaaag accaacaaaa agctaatcaa ttagcaagtg
    31321 aattaaacaa tcaaaaaatt cctcattttt ataattataa agaagtaatt catacgaaat
    31381 tgtataaaga taatttattt gatgtaaaag cgaaagaacc atacaatgta acaattacta
    31441 gtgataaata catccctaat actgatttga aacgtgggca agctgattta tttgtagcgg
    31501 aaggttctat caaagattta gtgaaacata agaagcatgg taaggcaatt ataggaacga
    31561 aaaaacatca tgttaatatt aagttacgta aagatattaa taaaatctat tttatgacag
    31621 atgttgattt aggtggacca acgtttgtct taaatgacaa agactatcaa gaaataagaa
    31681 agtatacaaa ggcaaagcat atcgtctctc aatttggatt cgatttgaaa cataaaaaag
    31741 atgctttagc attagaaaaa gcgaaaaata aagttgataa atctattgaa acaagaagtg
    31801 aagcgataag ctcaatatca agtttaaccg gaatattatt atttgtaaca tcatttttag
    31861 gtattacatt cttgattgct gtatgttgca ttatatacat aaagcaaata gatgaaaccg
    31921 aagatgagtt agagaattat agtattttga gaaagcttgg atttacacaa aaagatatgg
    31981 caaggggact aaagtttaaa attatgttta attttgggtt acctttagtt attgcactat
    32041 cacatgcata ttttacatca ttagcatata tgaaattaat gggtacaacg aatcaaatac
    32101 cggttttcat agtaatggga ttatacattt gtatgtatgc tgtttttgca gtgacggctt
    32161 ataatcattc caagcgaaca attagacatt ccatataaaa tatacagatg gctttcagta
    32221 gagtagtgga ttcggattca cgaactatac tggaagcttt ttattataaa tgaagagaag
    32281 ttatattttt agcatgtata gttgaatact gggttaaaat accatattaa taatgaagta
    32341 aaggtatgag tgattatgaa agtgttttga atgaaatata tttaattggt gatgctttta
    32401 attgaaaaga ttaacaggat tcaactttgt aaattgtatt aaatgtgaga aaataaaagt
    32461 atattcattg agagatatat gagtcaatga tcgttt
//
LOCUS       DALAGD010000023        30987 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.023, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000023 DALAGD010000000
VERSION     DALAGD010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 30987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30987
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.023"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            82..1563
                     /locus_tag="O2701_000825"
     CDS             82..1563
                     /locus_tag="O2701_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCZ4292731.1"
                     /translation="MKKIFMMVHELDVNKGGMTSSMFNRSKEFYDADIPADIVTFDYK
                     GNYDEIIKALKKQGKMDRRTKMYNVFEYFKQISNNKHFKSNKLLYKHISERLKNTIEI
                     EESKGISRYFDITTGTYIAYIRKSKSEKVIDFFKDNKRIERFSFIDNKVHMKETFNVD
                     NKVCYQVFYDEKGYPYISRNINANNGAVGKTYVLVNKKEFKNNLALCVYYLEKLIKDS
                     KDSIMICDGPGSFPKMFNTNHKNAQKYGVIHVNHHENFDDTGAFKKSEKYIIENANKI
                     NGVIVLTEAQRLDILNQFDVENIFTISNFVKIHNAPKHFQTEKIVGHISRMVPTKRID
                     LLIEVAELVVKKDNAVKFHIYGEGSVKDKIAKMIEDKNLERNVFLKGYTTTPQKCLED
                     FKLVVSTSQYEGQGLSMIEAMISKRPVVAFDIKYGPSDFIEDNKNGYLIENHNINDMA
                     DKILQLVNNDVLAAEFGSKARENIIEKYSTESILEKWLNLFNS"
     gene            complement(1652..2008)
                     /locus_tag="O2701_000826"
     CDS             complement(1652..2008)
                     /locus_tag="O2701_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCZ4292732.1"
                     /translation="MKLGMLLIRWMLATSFLVHGTLKFVDLDGTIHFLGSLGLPPTIA
                     VLMSIGEVVGGLALIIGFLTPYVNVFYICVMLGSIFTLKLTRGYVSGYEFEIIHIVMN
                     LACISSYSWKKWLQFY"
     gene            2485..3444
                     /locus_tag="O2701_000827"
     CDS             2485..3444
                     /locus_tag="O2701_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCZ4292733.1"
                     /translation="MNRNIVKLVVFMLILVVAVAGCGQKDTEEKTEMTTIKDELGTEK
                     IKKNPKRVVVLEYSFADYLAALDMKPVGIADDGSTKNITKSVRDKIGAYESVGSRPQP
                     NMEVISKLKPDLIIADVSRHKKIKSELSKIAPTIMLVSGTGDYNANIEAFKTVAKAVG
                     KEKEGEKRLEKHDKILAEIRKKIEQSTLKSAFAFGISRAGMFINNEDTFMGQFLIKMG
                     IQPEVTKDKTTHVGERKGGPYIYLNNEELANINPKVMILATDGKTDKNRTKFIDPAVW
                     KSLKAVKDNKVYDVDRNKWLKSRGIIASESMAEDLEKIAEKAK"
     gene            complement(3490..3621)
                     /locus_tag="O2701_000828"
     CDS             complement(3490..3621)
                     /locus_tag="O2701_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292734.1"
                     /translation="MRYVTMRQFIKRTVKTILVGYVIKFIRNKLSGKSSHPTDNKHN"
     gene            complement(3685..3900)
                     /locus_tag="O2701_000829"
     CDS             complement(3685..3900)
                     /locus_tag="O2701_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HCZ4292735.1"
                     /translation="MKVNKAIYVIVALFLGGLGIHKFYADKFGQGLFHLLFFWTGIPT
                     IISIIHAIFVIFTKKADKDGYIIFPKN"
     gene            4065..4616
                     /locus_tag="O2701_000830"
     CDS             4065..4616
                     /locus_tag="O2701_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4292736.1"
                     /translation="MIRQARPEDRFDIAKLVYMVWDDMELELVKHLPKDMVLDAIEKS
                     CVDATYRTFYQHILVYEVENKVAGCIISYSGENELKYEKAWELLDLPEKIKQYGTPLP
                     VKEAKDDEYYIETIATFAAYRGRGIATKLLTSLLESNTHVKWSLNCDINNEAALKLYK
                     KVGFISDGQIELYKHMYHHLIVK"
     gene            complement(4668..5606)
                     /locus_tag="O2701_000831"
     CDS             complement(4668..5606)
                     /locus_tag="O2701_000831"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCZ4292737.1"
                     /translation="MSNQYQQYSTVKKYWHLMRPHTLTASVVPVLVGTAASKIYFLGS
                     EDHIKISLFIAMLLACLLIQAATNMFNEYYDYKKGLDDHESVGIGGAIVRNGMSPELV
                     LRLAIAFYILAAILGLFLAANSSFWLLPVGLVCMAVGYLYTGGPFPISWTPFGELFSG
                     VFMGMFIIVIAFFIQTGNIQSYVIWLSVPIVITIGLINMANNIRDRVKDKASGRKTLP
                     ILLGKNASLTFMAIMYFIAYAFIVLTIIIKPGGSLFYLLALLSFPMPVKVIRRFKKND
                     TPPTMMPAMAAAGKTNTFFGLLYALGIYISALFAGI"
     gene            5788..7149
                     /locus_tag="O2701_000832"
     CDS             5788..7149
                     /locus_tag="O2701_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCZ4292738.1"
                     /translation="MATGVLEDDIVKEIYGSSKEWVSVEVKLSQSLDPSTLFHLTDNE
                     AGDRFYMRLNDNRTSYFGYKAIQLFKNNSKNKQSIFKDWEKLKHNITFIHPQSEKHHL
                     RVVGGFQFSSHKSDDEWREFGLNHFVLPEVLISTDNNGTFLTYTVKRESFTVEALNDL
                     MDLFNNISDIDVDEQIGGITRNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFD
                     KDISIPYILKQAYSKEKNSYIFLLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKR
                     SQDEDEDTKNVEAFLKDNKNLIEHRFVVDSILHDIKPYITELHYDKTPKILKNDHLYH
                     LYTEIKAPLKDDSYISLIDNLHPTPALGGYPKEFAMDFIEQKEFGTRGLYGAPVGYID
                     IYDDCEFIVAIRSMLIKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMN
                     GKS"
     gene            7136..8809
                     /gene="menD"
                     /locus_tag="O2701_000833"
     CDS             7136..8809
                     /gene="menD"
                     /locus_tag="O2701_000833"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCZ4292739.1"
                     /translation="MGNHKAALTKQVFTFASELYAYGVREVVISPGSRSTPLALAFEA
                     HPNIKTWIHPDERSAAFFAVGLIKGSERPVAILCTSGTAAANYTPAIAESQISRIPLI
                     VLTSDRPHELRSVGAPQAINQVNMFNNYVSYEFDMPIADDSKETIDAIYYQMQIASQY
                     LYGPHKGPIHFNLPFRDPLTPDLNATELLTSEMKILPHYQKSIDASALRHILNKKKGL
                     IIVGDMQHQEVDQILTYSTIYDLPILADPLSHLRKFDHPNVICTYDLLFRSGLDLNVD
                     FVIRVGKPVISKKLNQWLKKTDAFQILVQNNDKIDVFPIAPDISYEISANDFFRSLME
                     DTTVNRVSWLEKWQCLEKKGRKEIKCYLEQATDESAFVGELIKKTSEKDALFISNSMP
                     IRDVDNLLLNKNIDVYANRGANGIDGIVSTALGMAVHKRITLLIGDLSFYHDMNGLLM
                     SKLNNIQMNIVLLNNDGGGIFSYLPQKESATDYFERLFGTPTGLDFEYTAKLYQFDFK
                     RFNSVSEFKNATLLSETSTIYELITNREDNFKQHQILYQKLSEMIHDTL"
     gene            8796..9599
                     /gene="menH"
                     /locus_tag="O2701_000834"
     CDS             8796..9599
                     /gene="menH"
                     /locus_tag="O2701_000834"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCZ4292740.1"
                     /translation="MTHYKFYEANVETNQVLVFLHGFLSDSRTYHNHIEKFTDNYHVI
                     TIDLPGHGEDQSSMDETWNFDYITTLLDRILDKYKDKSITLFGYSMGGRVALYYAING
                     HIPISNLILESTSPGIKEEANQLERRLVDDARAKVLDIAGIELFVNDWEKLPLFQSQL
                     ELPVEIQHQIRQQRLSQSPHKMAKALRDYGTGQMPNLWPRLKEIKVPTLILAGEYDEK
                     FVQIAKKMANLIPNSKCKLISATGHTIHVEDSDEFDTMILGFLKEEQND"
     gene            9592..10413
                     /gene="menB"
                     /locus_tag="O2701_000835"
     CDS             9592..10413
                     /gene="menB"
                     /locus_tag="O2701_000835"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCZ4292741.1"
                     /translation="MTNRQWETLREYDEIKYEFYEGIAKVTINRPEVRNAFTPKTVAE
                     MIDAFSRARDDQNVSVIVLTGEGDLAFCSGGDQKKRGHGGYVGEDQIPRLNVLDLQRL
                     IRIIPKPVIAMVKGYAVGGGNVLNVVCDLTIAADNAIFGQTGPKVGSFDAGYGSGYLA
                     RIVGHKKAREIWYLCRQYNAQEALDMGLVNTVVPLEKVEDETVQWCKEIMKHSPTALR
                     FLKAAMNADTDGLAGLQQMAGDATLLYYTTDEAKEGRDAFKEKRDPDFDQFPKFP"
     gene            complement(10652..10981)
                     /gene="sspC"
                     /locus_tag="O2701_000836"
     CDS             complement(10652..10981)
                     /gene="sspC"
                     /locus_tag="O2701_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin B"
                     /protein_id="HCZ4292742.1"
                     /translation="MYQLQFINLVYDTTKLTHLEQTNINLFIGNWSNHQLQKSICIRH
                     GDDTSHNQYHILFIDTAHQRIKFSSIDNEEIIYILDYDDTQHILMQTSSKQGIGTSRP
                     IVYERLV"
     gene            complement(11019..12200)
                     /gene="sspB"
                     /locus_tag="O2701_000837"
     CDS             complement(11019..12200)
                     /gene="sspB"
                     /locus_tag="O2701_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain B"
                     /protein_id="HCZ4292743.1"
                     /translation="MNSSCKSRVFNIISIIMVSMLILSLGAFANNNKAKADSHSKQLE
                     INVKSDKVPQKVKDLAQQQFAGYAKALDKQSNAKTGKYELGEAFKIYKFNGEEDNSYY
                     YPVIKDGKIVYTLTLSPKNKDDLNKSKEDMNYSVKISNFIAKDLDQIKDKNSNITVLT
                     DEKGFYFEEDGKVRLVKATPLPGNVKEKESAKTVSAKLKQELKNTVTPTKVEENEAIQ
                     EDQVQYENTLKNFKIREQQFDNSWCAGFSMAALLNATKNTDTYNAHDIMRTLYPEVSE
                     QDLPNCATFPNQMIEYGKSQGRDIHYQEGVPSYEQVDQLTKDNVGIMILAQSVSQNPN
                     DPHLGHALAVVGNAKINDQEKLIYWNPWDTELSIQDADSSLLHLSFNRDYNWYGSMIG
                     Y"
     gene            complement(12282..13292)
                     /gene="sspA"
                     /locus_tag="O2701_000838"
     CDS             complement(12282..13292)
                     /gene="sspA"
                     /locus_tag="O2701_000838"
                     /EC_number="3.4.21.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-specific serine endopeptidase SspA"
                     /protein_id="HCZ4292744.1"
                     /translation="MKGKFLKVSSLFVATLTTATLVSSPAANALSSKAMDNHPQQTQS
                     SKQQTPKIQKGGNLKPLEQREHANVILPNNDRHQITDTTNGHYAPVTYIQVEAPTGTF
                     IASGVVVGKDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEG
                     DLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGKI
                     TYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHWGGVPNEFNGAVFINENVRNFLKQ
                     NIEDIHFANDDQPNNPDNPDNPNNPDNPNNPDEPNNPDNPNNPDNPDNGDNNNSDNPD
                     AA"
     gene            complement(13820..14974)
                     /locus_tag="O2701_000839"
     CDS             complement(13820..14974)
                     /locus_tag="O2701_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCZ4292745.1"
                     /translation="MKLSLNSNSKYLRAPSIRQFSNRMNNLDDCVNLTIGQPDFPMPD
                     VVKKAYIDAINNDKTSYSHNKGLLETREAISQYFKNRYHFSYDPEEIIVTNGASEAID
                     TTLRSIIEPGDEIIIPGPIYAGYIPLIEVLGGKPIYIDTTATQFKITPDALESHISPK
                     TKAVLLNYPTNPTGVVLKRNEVLNIVNVLKKYPLFIISDEIYAENTFSGKHVSFAEFE
                     DIRDQLILIGGLSKSHSATGIRIGFLLGPQYLIDKLTFMHAYNCICANVPAQIACITA
                     LNEGLEAPKYMNEAYVERRNYLVSELTKLGFEITAQPEGAFYIFPSIKHITDDDFEFC
                     VDLLESTHLAIVPGSSFTEFGKGFVRISYAYEMDVLKEGMKRLAKYLNTK"
     gene            complement(15170..16180)
                     /locus_tag="O2701_000840"
     CDS             complement(15170..16180)
                     /locus_tag="O2701_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCZ4292746.1"
                     /translation="MTSLKERDYFFDNARAILILLVVFGHMLQPYTSGDKYLSALYLV
                     IYSFHMPTFLFISGYFAKNIDKPYYLEKISKRLIVPYMIFFAFFSIYYFLTGKSDELQ
                     LDPFNPVFALWFLITLFFFHVILVIVRRFNPYKVLSVSIIISIGAGFSDNIDSYLSIS
                     RTIVFFPIFYLGYIFTKKHTAIFKNKKLIPVSIITFILFFIVYVIHPINADWLLGSSP
                     YTSLENEGQSIFSPFKRLILYGIILIAMTAFLNLMSTKKKLYTYIGSRTLYVYLLHGL
                     IIGIVRGFEWYPFDNPISLMTYLYLISISVIIVYVLSTNFVCKWTNPIINLQRPSQFK
                     DS"
     gene            16333..16752
                     /locus_tag="O2701_000841"
     CDS             16333..16752
                     /locus_tag="O2701_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCZ4292747.1"
                     /translation="MYKQLEKLITLTNNDLNLVNRRFGQRTDIKSEQLELLRILFNYD
                     RLSQYDLTMKISREQSIVSRWIKKLVLKGYITSQQSSEDLRCKELILTDQARTLISQI
                     NNARCELIEARCQCLSEVELDNLNQLLDKLNQRRISL"
     gene            16779..16889
                     /locus_tag="O2701_000842"
     CDS             16779..16889
                     /locus_tag="O2701_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292748.1"
                     /translation="MSLCTQQKLNHELQAKVAVIVKIDGKQSPLFLLVYV"
     gene            complement(16959..20729)
                     /locus_tag="O2701_000843"
     CDS             complement(16959..20729)
                     /locus_tag="O2701_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCZ4292749.1"
                     /translation="MAKKFNYKLPSMVALTLVGSAVTAHQVQAAETTQDQTTNKNVLD
                     SNKVKATTEQAKAEVKNPTQNISGTQVYQDPAIVQPKTANNKTGNAQVSQKVDTAQVN
                     GDTRANQSATTNNTQPVAKSTSTTAPKTNTNVTNAGYSLVDDEDDNSENQINPELIKS
                     AAKPAALETQYKTAAPKAATTSAPKAKTEATPKVTTFSASAQPRSVAATPKTSLPKYK
                     PQVNSSINDYIRKNNLKAPKIEEDYTSYFPKYAYRNGVGRPEGIVVHDTANDRSTING
                     EISYMKNNYQNAFVHAFVDGDRIIETAPTDYLSWGVGAVGNPRFINVEIVHTHDYASF
                     ARSMNNYADYAATQLQYYGLKPDSAEYDGNGTVWTHYAVSKYLGGTDHADPHGYLRSH
                     NYSYDQLYDLINEKYLIKMGKVAPWGTQSTTTPTTPSKPTTPSKPSTGKLTVAANNGV
                     AQIKPTNSGLYTTVYDKTGKATNEVQKTFAVSKTATLGNQKFYLVQDYNSGNKFGWVK
                     EGDVVYNTAKSPVNVNQSYSIKPGTKLYTVPWGTSKQVAGSVSGSGNQTFKASKQQQI
                     DKSIYLYGSVNGKSGWVSKAYLVDTAKPTPTPTPKPSTPTTNNKLTVSSLNGVAQINA
                     KNNGLFTTVYDKTGKPTKEVQKTFAVTKEASLGGNKFYLVKDYNSPTLIGWVKQGDVI
                     YNNAKSPVNVMQTYTVKPGTKLYSVPWGTYKQEAGAVSGTGNQTFKATKQQQIDKSIY
                     LFGTVNGKSGWVSKAYLAVPAAPKKAVAQPKTAVKAYTVTKPQTTQTVSKIAQVKPNN
                     TGIRASVYEKTAKNGAKYADRTFYVTKERAHGNETYVLLNNTSHNIPLGWFNVKDLNV
                     QNLGKEVKTTQKYTVNKSNNGLSMVPWGTKNQVILTGNNIAQGTFNATKQVSVGKDVY
                     LYGTINNRTGWVNAKDLTAPTAVKPTTSAAKDYNYTYVIKNGNGYYYVTPNSDTAKYS
                     LKAFNEQPFAVVKEQVINGQTWYYGKLSNGKLAWIKSTDLAKELIKYNQTGMTLNQVA
                     QIQAGLQYKPQVQRVPGKWTDANFNDVKHAMDTKRLAQDPALKYQFLRLDQPQNISID
                     KINQFLKGKGVLENQGAAFNKAAQMYGINEVYLISHALLETGNGTSQLAKGADVVNNK
                     VVTNSNTKYHNVFGIAAYDNDPLREGIKYAKQAGWDTVSKAIVGGAKFIGNSYVKAGQ
                     NTLYKMRWNPAHPGTHQYATDVDWANINAKIIKGYYDKIGEVGKYFDIPQYK"
     gene            complement(20957..21391)
                     /locus_tag="O2701_000844"
     CDS             complement(20957..21391)
                     /locus_tag="O2701_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4292750.1"
                     /translation="MFSKVNNQKMLEDCFYIRKKVFVEEQGVPEESEIDEYESESIHL
                     IGYDNGQPVATARIRPINETTVKIERVAVMKSHRGQGMGRMLMQAVESLAKDEGFYVA
                     TMNAQCHAIPFYESLNFKMRGNIFLEEGIEHIEMTKKLTSLN"
     gene            complement(21546..22016)
                     /locus_tag="O2701_000845"
     CDS             complement(21546..22016)
                     /locus_tag="O2701_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499548.1"
                     /note="Essential protein for resistance to osmotic stress
                     from salt; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic stress response protein"
                     /protein_id="HCZ4292751.1"
                     /translation="MSRKTYEKIANINGMFNMLEQQIIHSQDMAHFRSEFFYVNHEHR
                     ENYEALLIYYKNSIDNPIVDGACYILALPEIFNSVDVFESELPFSWVYDENGITETMK
                     SLSIPLQYLVAAALEVTDVNIFKPSGFTMGMNNWNIAQMRIFWQYTAIIRKEAL"
     gene            complement(22064..23281)
                     /locus_tag="O2701_000846"
     CDS             complement(22064..23281)
                     /locus_tag="O2701_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase"
                     /protein_id="HCZ4292752.1"
                     /translation="MNKFLKYFLILLALVLIVVPIVFATLLFKTSQDAFESSQDSKNA
                     NRQSNLRDNKVNPEEQPISILFLGIDDNDGRRKKGQDAEHSRSDAMILTTFNQSKHQI
                     RMLSIPRDTISYIPKVGYYDKITHAHAYGGPIAAMDSVEATMNVPVDYYVRVNMKAFV
                     EAVNELGGIYYDVPYDLNEPNTDDTGKIKIKKGYQKLNGDEALAVARTRHHDSDLKRG
                     QRQMELIKILFQKAQEVDSIDKLDNVIQIVGKNAKHNLTNSEIKALAKMYLTNDVEIK
                     TAQLKGKDDMLNGIYYYHPSVESIQKYANLLRKDLELSPINDKNDFLDQRVINHYGSL
                     IPLTPLDNSLLRKEQNDTTDKDKTSNENSDSTNNSDSSNQQQPATDQNSNQNQGGTQQ
                     APQASNNQNGVVN"
     gene            23732..24925
                     /gene="fmtA"
                     /locus_tag="O2701_000847"
     CDS             23732..24925
                     /gene="fmtA"
                     /locus_tag="O2701_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala esterase FmtA"
                     /protein_id="HCZ4292753.1"
                     /translation="MKFNKVKLVIHACVLLFIIISIALIFHRLQTKTHSIDPIHKETK
                     LSDNEKYLVDRNKEKVAPSKLKEVYNSKDPKYKKIDKYLQSSLFNGSVAIYENGKLKM
                     SKGYGYQDFEKGIKNTPNTMFLIGSAQKFSTGLLLKQLEEEHKININDPVSKYLPWFK
                     TSKPIPLKDLMLHQSGLYKYKSSKDYKNLDQAVKAIQKRGIDPKKYKKHMYNDGNYLV
                     LAKVIEEVTGKSYAENYYTKIGDPLKLQHTAFYDEQPFKKYLAKGYAYNSTGLSFLRP
                     NILDQYYGAGNLYMTPTDMGKLITQIQQYKLFSPKITNPLLHEFGTKKYPDEYRYGFY
                     AKPTLNRLNGGFFGQVFTVYYNDKYVVVLALNVKGNNEVRIKHIYNDILKQNKPYNTK
                     GVIVQ"
     gene            complement(25462..25740)
                     /gene="qoxD"
                     /locus_tag="O2701_000848"
     CDS             complement(25462..25740)
                     /gene="qoxD"
                     /locus_tag="O2701_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit IV"
                     /protein_id="HCZ4292754.1"
                     /translation="MKHTVGFIASIVLTLLAVYVTLYTSLTFHAKLTIIFGFAFVQAG
                     LQLLMFMHLTEGKDGRLQTFKVIFALVITLCFVVGTYWVMQGGHSSHL"
     gene            complement(25749..26354)
                     /gene="qoxC"
                     /locus_tag="O2701_000849"
     CDS             complement(25749..26354)
                     /gene="qoxC"
                     /locus_tag="O2701_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit III"
                     /protein_id="HCZ4292755.1"
                     /translation="MSHDTNTIDSRTHEGELNKLGFWIFITAEFALFGTLFATLLTLQ
                     HGGDYAGKMTTELFELPLVLIMTFALLFSSYTCGIAIYYMRQEKQKLMMFWMIITLLL
                     GLVFVGFEIYEFAHYASEGVNPTIGSYWSSFFILLGTHGCHVSLGIVWAICLLIQIQR
                     RGLDKYNAPKLFIVSLYWHFLDVVWVFIFTAVYMIGMVYSG"
     gene            complement(26344..28332)
                     /gene="qoxB"
                     /locus_tag="O2701_000850"
     CDS             complement(26344..28332)
                     /gene="qoxB"
                     /locus_tag="O2701_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit I"
                     /protein_id="HCZ4292756.1"
                     /translation="MNFPWDQLLVKGNWMITMAQIGAPFLVIGLIAVITYFKLWKYLY
                     KEWFTSVDHKKIGIMYLICAVLMFVRGGIDALLIRAQLTVPDNKFLESNHYNEIFSTH
                     GVIMIIFMAMPFIFGLWNIVVPLQIGARDVAFPVLNNVSFWLFFAGMILFNLSFIIGG
                     SPAAGWTNYAPLAGEFSPGPGVNYYLIAIQISGLGTLATGINFFVTILRCKTPTMKFM
                     QMPMFTVTTFITTLIVILAFPPLTVALALMTTDRIFDTAFFTVAHGGMPMLWANFFWV
                     WGHPEVYIVILPAFGIYSEIIPTFARKRLFGHQSMVWATAGIAFLSFLVWVHHFFTMG
                     NGALINSFFSISTMLIGIPTGVKLFNWLLTLYKGRITFESPMLFSLAFIPNFLLGGVT
                     GVMLAMASADYQYHNTYFLVAHFHYTLVTGVVFACLAGLIFWYPKMMGYKLNETLNKW
                     CFWFFMIGFNVCFLPQFILGLDGMPRRLYTYMPSDGWFLLNLISTIGALLMAIGFLFL
                     VVSIVYSHFKSPREATGDNWDGLGRTLEWTTASAIPPKYNFAITPDWNDYDTFVDMKE
                     HGRHYLDNHNYKDIHMPNNTPVGFWIGIFMTIGGFFLIFETVIPALICLFGIFGTMIY
                     RSFQIDHGYHIPAAEVAETEARLREARIKEREAVSHES"
     gene            complement(28332..29432)
                     /gene="qoxA"
                     /locus_tag="O2701_000851"
     CDS             complement(28332..29432)
                     /gene="qoxA"
                     /locus_tag="O2701_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit II"
                     /protein_id="HCZ4292757.1"
                     /translation="MSKFKSLLLLFGTLILLSGCSNIEIFNAKGPVASSQKFLILYSI
                     VFMLVICFVVLGMFAIFIYKYSYNKNAESGKMHHNAIIETIWFVIPIIIVAALAIPTV
                     KTLYDYEKPPKSEKDPMVVYAVSAGYKWFFAYPDEHIETVNTLTIPKDRPVVFKLQAM
                     DTMTSFWIPQLGGQKYAMTGMTMNWTLEASQTGTFRGRNSNFNGEGFSRQTFKVNAVS
                     QKDYDKWVKEVKGKKTLDQDTFDKQLLPSTPNKALEFNGTHMAFVDPAADPEYIFYAY
                     KRFNFELKDPNFTSEENMFKDVSDKPLIPARKAQITNANYKRHGMKLMILGNDEPYNN
                     EFKKDESKNAKEMKKISKDAQDQDNDDHGGGH"
     gene            29734..29856
                     /locus_tag="O2701_000852"
     CDS             29734..29856
                     /locus_tag="O2701_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292758.1"
                     /translation="MIERKRKHFNKEFAKSNLSLNNEKWKFVCSKSKHFFKKSS"
     gene            complement(30004..30321)
                     /locus_tag="O2701_000853"
     CDS             complement(30004..30321)
                     /locus_tag="O2701_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HCZ4292759.1"
                     /translation="MNKLLQSLSALGVSATLVTPNLNADATTNTTPQIKGANDIVIKK
                     GQDYNLLNGISAFDKEDGDLTDKIKVDGQIDTSKSGKYQIKYHVTDSDGAIKISTRYI
                     EVK"
BASE COUNT        10645 a         4556 c         5310 g        10476 t
ORIGIN      
        1 taatttatat gttgaagaag aattttatca tacatataac gaattaattg ttaaatatac
       61 tgaggtaaag gaataattat aatgaaaaaa atatttatga tggtacatga gttagatgtg
      121 aataaaggtg gtatgacctc ttcgatgttc aatagaagta aagagtttta tgatgcggac
      181 atacctgctg atattgttac tttcgattac aaaggaaact atgatgaaat tattaaagct
      241 ttgaaaaaac aaggtaaaat ggatcgaaga acgaaaatgt ataatgtatt tgagtatttt
      301 aaacaaattt caaataataa acattttaag tctaataaat tgttatataa acatatttca
      361 gaaagactaa aaaatacgat tgaaattgaa gagagtaaag gtatttcaag atattttgat
      421 ataacgactg gtacatatat tgcctacatt agaaaaagta aatctgaaaa agtgattgat
      481 ttctttaaag ataataaacg aattgaacgg tttagtttta tagataataa agtgcatatg
      541 aaggaaacat ttaatgtaga taataaagtt tgttatcaag tattttatga tgaaaaggga
      601 tacccatata tttcaaggaa tattaatgct aataatggtg ctgtaggtaa aacttatgtg
      661 ttagttaata aaaaagaatt taaaaacaat ttagcactgt gtgtttacta tttagaaaaa
      721 ctaataaaag attctaaaga tagtattatg atttgtgatg gaccagggag ttttccaaaa
      781 atgtttaata caaatcataa aaatgctcag aaatatggcg ttattcatgt taatcatcat
      841 gaaaatttcg atgatacggg tgcatttaaa aaaagtgaga aatatattat tgagaatgcg
      901 aataaaatta acggtgtaat tgtattaaca gaggcacaaa gattagatat tcttaatcaa
      961 tttgatgtag aaaatatttt cactattagc aattttgtta agatacataa tgctccaaaa
     1021 cattttcaaa ctgaaaaaat cgtaggtcat atttctagaa tggtaccaac gaagcgaatt
     1081 gatttgctta ttgaagtggc tgagttagtc gtaaaaaaag ataatgctgt taaatttcat
     1141 atatatggag aaggatctgt caaagataaa atagctaaaa tgattgaaga taaaaattta
     1201 gaaagaaatg tttttcttaa aggatataca acaactccac aaaaatgctt ggaagatttt
     1261 aaattagtcg tttctacatc tcaatatgaa ggtcaagggt taagtatgat agaagcaatg
     1321 atttctaaaa ggcctgttgt tgcctttgac atcaaatacg gaccaagtga ttttatagaa
     1381 gataataaaa atggttattt aatagaaaac cataatatta acgacatggc tgataaaata
     1441 cttcagcttg ttaataatga tgtattagca gcggagtttg gttcgaaagc gagagaaaac
     1501 attatagaaa aatattcaac ggaatcaata ttagaaaaat ggttaaatct tttcaatagc
     1561 taaatgaaaa aatcctaggt attcatgagt acctaggatt tttacgcatg ataaatacaa
     1621 aaaaagagag gatagtcctc tcaaacaata gttaataaaa ttgtagccat ttcttccagc
     1681 tgtaactact aatacaagct aagttcatta cgatatgtat gatttcaaat tcataaccac
     1741 ttacatatcc tcttgtaagt ttaagtgtga agattgagcc taacataaca caaatataaa
     1801 aaacattaac ataaggcgtt aaaaagccga taataagtgc taaacctcca acaacttcac
     1861 caattgacat caatactgcg atggtaggtg gtaaacctaa agagcctaag aagtgaattg
     1921 ttccatctaa atcaacaaat ttcaaagtac catgcactaa gaatgatgtt gctaacatcc
     1981 atctaattaa aagcatacct aatttcatca tgtcacctcc aacattcata tttttaaaac
     2041 atagtccaaa ttatatttgt aaacatgtgg aaagtaaaga ttttttataa gattaattat
     2101 taaatattct attgtattga acaattgttg tagaaagatg tttaagtcat cagttacttt
     2161 ttgtatttat aaataaagat tagtacagca tagtgcaaag attataggga aatgaattaa
     2221 ctattgcatc gctatttatg aatcgtaaac actattatga aattgcgaga gtagtatgga
     2281 caattagaat gatggtttga gataaatgag tgttattaac cagtgcgtca ttttcaaaat
     2341 tagatgccat ttgctgttta taataagaag atttgataat taactatgat aagaaaaaat
     2401 aataatcccc taaatacatt ataaacaaat taaatgataa aataataata tatatttcta
     2461 ctaaagttgt ttggaggtaa atgtgtgaat aggaatatcg ttaaactagt tgtgttcatg
     2521 ctaattttag ttgtagcagt agcgggttgt ggtcaaaaag atactgaaga gaaaactgaa
     2581 atgacgacaa taaaagatga attaggaact gaaaaaatta agaaaaatcc taaacgtgtt
     2641 gttgtattag aatatagttt tgctgattat ttagcagcat tagatatgaa acctgttggt
     2701 attgcagatg atggcagcac taaaaatata acaaagtcag taagagataa gattggggca
     2761 tatgaatcgg ttggatctag accgcaaccg aatatggaag tgataagtaa attaaaaccg
     2821 gatttgatca ttgcagatgt tagcagacat aagaaaatca aatcagaatt gagcaaaatt
     2881 gctccgacaa tcatgttagt tagcggtacg ggagattata atgcaaatat tgaagcattt
     2941 aaaacagtcg ctaaagcagt aggcaaagag aaagaaggcg agaagcgtct ggaaaagcat
     3001 gataaaatat tagcggagat tagaaagaaa attgaacaga gtacgttaaa atctgcattt
     3061 gcattcggta tctcaagagc aggtatgttt attaataatg aagatacatt tatgggacaa
     3121 ttcttaatta aaatgggtat tcaacctgaa gtcacaaaag acaaaactac gcatgttggt
     3181 gaacgcaagg gtggtcctta tatatattta aataatgaag aacttgccaa tatcaatcca
     3241 aaagttatga ttttagccac tgacggaaaa acggacaaaa atagaacgaa attcattgat
     3301 cctgcagttt ggaaatcatt aaaagctgtg aaagataaca aagtttatga cgttgaccga
     3361 aataagtggt tgaaatcaag ggggattatc gcaagtgaaa gtatggcaga agatttagaa
     3421 aaaattgcag aaaaagcaaa ataaaaatac agcgctactc gtaaatcata taagagtggc
     3481 gctgtatttt taattatgtt tattatctgt cggatgtgat gatttacctg aaagtttatt
     3541 tcgaataaat ttaattacat aaccgacaag gattgtttta acagttcttt taatgaattg
     3601 gcgcatcgtt acatacctca tttctctata tcttacgaac tatataccca ttcatatatg
     3661 ctttttaaac gtcattgtca caatttaatt tttagggaat ataatataac catctttatc
     3721 tgctttttta gtaaaaatga caaaaattgc atgtattatt gagatgatgg tagggatacc
     3781 tgtccagaaa aataataagt gaaaaagacc ttgtccaaat ttatcagcat aaaatttatg
     3841 aatacctaaa cctccaagaa ataatgcaac aataacataa atggctttat tgactttcat
     3901 ttgtaaatcc tccttaacta taattctact taaattcgtt gtgaaaacca atatttctaa
     3961 ctttagaatt ttcaaacttt ctaaaattat aagtatatct ttttaaaata agctagaatt
     4021 tctatataat aaatgttaat aacgtaaaag ggaatgatga catagtgata cgtcaagcac
     4081 gtccagagga ccgatttgat attgcgaagt tagtttatat ggtttgggat gatatggaat
     4141 tagaattggt aaagcatcta cctaaagaca tggtattaga tgcaattgaa aaaagctgtg
     4201 ttgatgcaac atatcgaact ttttatcagc atattttagt ttatgaagta gaaaataaag
     4261 tagcaggttg tattattagc tatagtggtg aaaatgaatt gaaatacgaa aaagcatggg
     4321 aactacttga cttgccagaa aaaataaaac aatatggcac gccattacct gtaaaagaag
     4381 ctaaagacga tgagtattat atagaaacaa ttgcgacatt tgcagcatat agaggtagag
     4441 gcatcgcgac aaagttatta acgtcattac ttgaatcaaa tacacatgtt aaatggagtt
     4501 tgaattgcga tattaataat gaagcagcat taaagttata taaaaaagta ggctttatat
     4561 ctgatggaca gattgaatta tacaagcaca tgtatcatca tttaattgtt aaataaaaaa
     4621 actcgacagt tcgatgtaag tcgattgccg agtagtatca ttttctatta aatgcctgca
     4681 aataatgcac taatataaat acctaatgca tataataaac cgaaaaatgt atttgtttta
     4741 ccagcagcag ccattgctgg catcattgta ggcggtgtat cattcttctt gaaacgtctg
     4801 ataactttaa caggcattgg gaatgataac aacgcaagta agtaaaataa tgagccacca
     4861 ggtttaataa tgatcgtaag tacaataaag gcataagcga taaagtacat gattgccata
     4921 aatgttaaag aagcattttt acctaataga atgggtaaag ttttgcgacc acttgcttta
     4981 tctttgacac ggtcgcgaat attgttagcc atattaatta aaccgatagt gattactata
     5041 ggtacactta accaaattac ataactttga atattgccag tttgaataaa gaatgcaata
     5101 acgataataa acatacccat aaatacgcct gagaataatt caccgaaagg cgtccatgaa
     5161 atagggaaag ggccacctgt atataggtaa ccaacagcca tacatactaa tccaactggt
     5221 aataaccaaa atgaagagtt agcagctaaa aacaaaccta atattgctgc taagatgtaa
     5281 aatgcaatgg ctaatcgtag cacaagctct gggctcatac cgttgcgaac aatggcacca
     5341 ccaatgccta cagattcatg atcatcaagg ccttttttat aatcatagta ttcattaaac
     5401 atattagttg ctgcttgaat aagtaagcat gctagtaaca tggcaatgaa taggctgatt
     5461 ttaatatgat cttcgctacc aagaaaatat attttagatg ctgctgtacc aactaaaacg
     5521 ggtactacgg aagcagttaa tgtatgagga cgcattaaat gccaatattt cttaactgta
     5581 gaatattgct gatattgatt actcattgaa tacaaaacct ctttaaatca tattttttat
     5641 aacaaagata ttttaataga aagtagcata aaggtcaatt gacgtgaaaa attgcaagat
     5701 aagtttaagc aattcttgta acacaattat ccaaaaatga tacaatataa taatgagtga
     5761 aaaaagacga agaaagaagt gaaatggatg gctacgggcg tattagagga cgatattgtc
     5821 aaagagatat atggcagctc aaaggaatgg gtttcagttg aagtgaaatt atcacagtca
     5881 ctagacccga gcacattatt tcatctcact gacaatgagg caggagatcg cttttatatg
     5941 cgtttgaatg ataatcgaac gtcatatttt ggctacaaag caattcaatt attcaaaaat
     6001 aattctaaaa ataaacaatc tatttttaaa gactgggaaa aattaaaaca taacatcaca
     6061 tttatacatc cgcaatctga gaaacatcat cttcgagttg ttggagggtt ccaattttca
     6121 agtcataaat cagatgatga atggcgagag tttggactaa atcattttgt attacctgaa
     6181 gttttaattt caactgataa taatgggaca tttttaactt atacagttaa aagggaaagt
     6241 tttactgttg aggcattgaa cgatttaatg gatttgttca acaatatatc ggacatagat
     6301 gtggacgagc aaattggggg aattactaga aatgaagata tttataaaga tgactggcgt
     6361 caacttgtag tagaagctat agaatctatt aataatgaag aaaaaattgt actagcacgt
     6421 agacggttaa taaagttcga taaagatatc agtattccat atattctaaa gcaagcatat
     6481 tctaaagaaa aaaacagtta tatattcttg ttagaatcac aagattctat attcttttca
     6541 caaacacctg aacaattaat aaaggtcaat aataaaatac tatcgactaa agctgtagca
     6601 ggtacaatta aacgttcaca agatgaggac gaagatacaa aaaatgttga agcattttta
     6661 aaagataata aaaacttaat cgaacatcga tttgttgttg acagtatttt acatgatatt
     6721 aaaccttata tcactgaatt acattatgat aagacgccta aaattctaaa aaatgatcat
     6781 ttatatcact tgtacactga aataaaggcg ccactgaagg atgattcgta tattagttta
     6841 attgataatt tacatccaac acctgcttta ggtggctatc caaaagaatt tgcaatggat
     6901 tttattgagc agaaagaatt tggtacgcga ggattatatg gtgcaccggt tggctatata
     6961 gatatatacg atgattgtga atttattgtt gcaattcgtt cgatgcttat taagaaagca
     7021 caagcaactt tatttgctgg gtgtggcatt gttaaagatt ctgatccaga tagtgaattg
     7081 gcagaaacga accttaagtt cacacctatg atgaatgcat taggagtcga tatgaatggg
     7141 aaatcataaa gcagctttaa cgaagcaagt ttttacattt gcatctgagt tatatgcgta
     7201 cggcgtaagg gaagtagtta tcagtccggg atcacgctca acgccacttg cacttgcatt
     7261 tgaagcacat ccaaatatta aaacatggat acaccccgat gagcgaagtg cagcgttttt
     7321 tgcagttggg ttaattaaag gcagtgaaag acctgtcgct atattatgta cgtcaggtac
     7381 agcagcagcg aattatacgc ctgcaattgc tgaaagccaa attagtagaa ttcctttaat
     7441 cgttttaaca agtgaccgtc cgcatgaatt aagaagtgta ggcgcaccac aagcgattaa
     7501 tcaagtaaat atgtttaata attatgtaag ttatgagttc gatatgccta ttgcggatga
     7561 tagtaaagag accattgatg caatttacta tcaaatgcaa attgctagtc aatatttata
     7621 tggaccacat aaagggccaa ttcattttaa cttgccattt agagatccgt taacacctga
     7681 tttgaatgca acagaattgt taacttctga gatgaagatt ttaccgcact atcaaaaaag
     7741 tatagatgca tcggcattaa gacacatttt aaataagaaa aaaggtttaa ttattgtagg
     7801 ggatatgcag caccaagaag ttgatcaaat actaacgtat tcaacgatat atgatttgcc
     7861 tattttagct gatcctttaa gtcatttaag aaaatttgat catccgaatg ttatctgtac
     7921 atatgatttg ctgtttagaa gcggcttaga cttaaatgtg gatttcgtaa ttcgtgttgg
     7981 gaaaccagtg atttctaaaa agttaaatca atggttaaag aaaactgatg catttcaaat
     8041 attagtgcaa aacaatgata agattgatgt ctttccgata gcgccagata tttcatatga
     8101 gatttctgcg aatgatttct ttaggtcatt aatggaagac acgacagtta atcgcgtaag
     8161 ttggttagaa aaatggcaat gcttagagaa aaaagggcgt aaagaaatta aatgttattt
     8221 ggaacaagct acagatgaga gtgcattcgt tggtgaattg attaagaaaa catctgaaaa
     8281 agatgcatta tttattagta atagtatgcc tatcagagat gtagataact tgttattgaa
     8341 taaaaatata gatgtctatg cgaatcgtgg tgcgaatggt attgatggta tcgtttcaac
     8401 tgcactgggt atggctgtgc ataaacgaat aacattattg ataggtgatt tatcatttta
     8461 tcatgatatg aatggactat taatgtcaaa attaaataat attcagatga atattgtatt
     8521 attgaacaac gatggtggcg gtattttttc atatttacca caaaaagaaa gtgcaactga
     8581 ctattttgaa cggttgtttg gcacaccgac gggattggat ttcgagtata cagctaagtt
     8641 atatcaattc gattttaaac gttttaacag tgtttcagaa tttaaaaatg ccacattgtt
     8701 atctgaaact tcgacgattt atgaattgat aacgaatcgc gaagataact ttaaacagca
     8761 tcaaatttta taccagaaat tgagtgaaat gattcatgac acattataaa ttttatgaag
     8821 caaacgttga gaccaatcaa gttttagtat ttctgcatgg atttcttagc gacagccgta
     8881 cttatcataa tcacatcgaa aaatttactg ataactatca tgtcatcact atagacttac
     8941 caggccatgg cgaagatcag tcttcaatgg atgaaacgtg gaattttgat tatattacga
     9001 cgttgttaga ccgaatttta gataaatata aagataaatc aataacattg tttggatatt
     9061 caatgggtgg gcgtgttgca ttatattatg caattaatgg tcacatccct atatctaatt
     9121 tgatattaga aagtacgtca ccaggtatta aagaagaagc aaatcaattg gaacgccgtc
     9181 ttgttgatga tgcacgtgct aaagtattag acatagcagg tattgaatta tttgttaatg
     9241 attgggaaaa gttgccatta tttcaatcgc aactagaatt accagttgaa atacaacatc
     9301 aaataagaca acaacgattg tctcaatcgc cacataaaat ggccaaagca ttaagagatt
     9361 atggtacagg tcaaatgcca aacttatggc cgcgcctgaa agaaattaaa gtaccaacat
     9421 taatattagc tggagaatat gatgaaaaat ttgtacagat tgcgaaaaaa atggcaaatt
     9481 taattcctaa tagtaaatgt aaattaattt ctgctacagg tcatacaatt catgtggaag
     9541 atagtgatga atttgataca atgatattag gatttttaaa ggaggagcaa aatgactaac
     9601 agacaatggg aaacacttag agaatatgat gaaatcaaat atgaatttta cgaagggatt
     9661 gctaaggtaa caataaatcg ccctgaagta cgcaatgcgt ttacacctaa aacagttgct
     9721 gaaatgattg acgcattttc acgtgcacgt gatgatcaaa acgtttcagt tatcgtatta
     9781 actggtgaag gtgatttagc attctgttct ggtggtgacc agaagaaacg tggacatggt
     9841 ggttatgtag gtgaagacca aatccctcgc ttaaatgtat tagatttaca gcgtttaatt
     9901 cgtattattc caaaaccggt tatcgcgatg gtaaaaggtt atgctgtagg tggcggtaat
     9961 gtactaaatg ttgtttgtga cttaacgatt gctgctgata atgctatttt tggacaaact
    10021 ggtcctaaag taggttcatt tgatgcgggt tatggttcag gatatttagc acgtatcgtt
    10081 ggacataaga aagcacgtga aatttggtac ttatgtcgtc aatacaatgc acaagaagct
    10141 ttagatatgg gtctagtaaa tacagtggta cctttagaga aagttgaaga tgaaactgtg
    10201 caatggtgta aagagattat gaaacactca ccaacagcgt tacgattcct taaagcagct
    10261 atgaatgctg acacagatgg tttagctggt ttacaacaaa tggctgggga tgcaacattg
    10321 ctttattaca caactgatga agcgaaagaa ggccgtgatg cgtttaaaga aaaacgtgat
    10381 cctgacttcg atcaattccc taaattccca taagttattt tgaatatgat attaagtcac
    10441 ttgcgtcgtt tattagcgac gataggtggc ttttttattt tataagaatt taaaagggca
    10501 ttttaattaa ttatcataaa gataagatag tttgagaaaa attaatagag aaagaataaa
    10561 tgttaaagat tattattaca atgaaaaaac gagctggtga gaaattgaat tctcgccaac
    10621 ccgctttatg aagtgaaatc atttaaatta gttatactaa gcgctcataa acgattggtc
    10681 gcgaagtgcc aataccttgt ttggatgacg tttgcatgag gatatgctgt gtatcatcat
    10741 aatcaagaat ataaatgatt tcttcattat caatagatga aaatttaatg cgttgatgtg
    10801 ccgtatctat aaaaagaata tgatattgat tgtgacttgt atcatcgcca tgacgtatac
    10861 aaattgattt ttgtaattga tgattactcc aattaccaat gaataaattg atattggttt
    10921 gttctagatg tgtgagtttg gttgtgtcgt aaactaaatt tataaattgt agttgataca
    10981 tagttttacc tgcctttaat caatatctat attacttttt agtaacctat cattgaacca
    11041 taccagttat aatcacgatt gaatgataaa tgtaataggc ttgaatctgc atcttggatt
    11101 gataattctg tatcccaagg attccagtaa ataagttttt cttggtcatt aattttagca
    11161 ttaccaacaa ctgctagcgc atgtcctaaa tgtgggtcat tagggttttg agatacactt
    11221 tgtgcaagga tcataattcc tacattatct tttgtaagtt gatcaacttg ttcatatgat
    11281 ggtacgcctt cttgataatg aatatctctg ccttgtgatt taccgtattc aatcatttga
    11341 ttagggaatg ttgcacaatt aggaaggtct tgctcactta cttcagggta taatgtacgc
    11401 ataatatcat gtgcattata agtgtctgta tttttagttg catttaataa tgctgccata
    11461 ctgaatcctg cacaccatga gttatcgaat tgttgttctc taattttgaa gttttttaat
    11521 gtattttcat attgaacttg atcttcttgt atcgcttcgt tttcttcaac tttagtaggt
    11581 gttactgtat tttttaactc ttgtttcaat tttgctgaaa ctgttttagc actttctttt
    11641 tcttttacat taccaggtag aggcgtagct tttactaatc taactttgcc atcttcttca
    11701 aaataaaacc ctttttcatc agtaagaaca gtgatatttg aatttttatc tttaatttgg
    11761 tctaaatctt tagcgatgaa gtttgaaatt ttaacactgt aattcatgtc ttcttttgat
    11821 ttgtttaaat catctttatt tttaggacta agtgttaaag tataaacaat tttaccgtct
    11881 tttataactg gataatagta actattatct tcttcaccat taaatttata aattttaaaa
    11941 gcttcgccaa gttcatattt accagttttt gcattacttt gtttatctaa tgctttagca
    12001 taaccagcaa attgttgttg tgctagatct tttacttttt gaggtacttt gtcactctta
    12061 acattaattt ctagctgttt agagtgtgaa tcggctttgg ctttattgtt attagcaaat
    12121 gcgcctagtg ataaaataag cattgaaacc attatgatgc ttataatatt gaatactcta
    12181 gatttacatg aactattcat ataataccct ccatttaaat caaaatattt tacgttgcta
    12241 agactttgct tctattttat ctagacgaac ttaattgtta attaagctgc atctggattg
    12301 tctgaattat tgttatcgcc attgtctgga ttatcagggt tgttagggtt gtcagggtta
    12361 tttggttcat ctgggttgtt agggttatca ggattgttag ggttatcagg attatctggg
    12421 ttattaggtt ggtcatcgtt ggcaaaatgg atatcttcaa tattttgttt taagaagttg
    12481 cgtacatttt cattaataaa taccgcacca ttaaattcat ttggtacacc gccccaatga
    12541 attccgatca cttcattttt ttcattaaat acaggtgaac ctgaattacc accagttgta
    12601 cttaaatcat attgcatagc ttcgcctttg aggtaagtga tttttccttt actttcccac
    12661 attgttgcta caggtttatc accaggatat cctgttacag taatattttg gttaacttgt
    12721 gtttcagcat tattactcat tgttgctggt ttaactactt caccaatatg tttgttttgc
    12781 tcattagggg agaatttaac tattgctaaa tcaccttcgc ctgaatattt agtgatttgt
    12841 tcagcagtga aaccaccatt tggataattg tcttggttaa ttgcagaagg gaatgctttt
    12901 aaagcatgag gatcaccgtg cgtagcatct acgacgtgtt tatttgttaa aagagtatct
    12961 ttacctacaa ctacaccgga agcaataaat gtaccagtag gtgcttcaac ttgaatataa
    13021 gttacgggtg cataatgacc attcgttgta tctgtgattt ggtgacgatc gttatttggt
    13081 aatataacat ttgcgtgttc acgttgttct aatggtttaa ggttaccgcc tttttgaatc
    13141 ttaggtgttt gctgtttgct tgactgcgtt tgttgtggat gattgtccat agcctttgaa
    13201 gataacgcgt ttgctgctgg agaactcaca agtgtcgctg ttgtcaaagt tgcaacgaat
    13261 aaagaactaa cttttaaaaa tttacctttc atctaaaaac ctccaaaaaa tttatttaca
    13321 agttaaatat aacactaaaa atttttaagt caataagaat atataaataa attaaaaagt
    13381 ttttgtaaag attagagttt acaaaaacat taagggaatt aaaattgaat tgtattatac
    13441 tttattcaat tgagttaaat tgataagatc gtcaattgtt tgcatttatc taaaagaata
    13501 aataagaaag gaattgagag cgaatggaaa gtatttgttt ataataataa atagtaactt
    13561 ttgaataata attgtttggc caaaggtaga ttaatatgaa tggtgatata ttatttgagt
    13621 gcttaagttt cgaagaacaa ttataagtta gcatattaaa accttttgat agaatcattt
    13681 tgagtgaggt gtttaaagga gaggaatata actcggtagt atttacagtc tctatagagt
    13741 attatttaaa acttatagaa tataaaaaac tgaacgtact atgaatttag ttcacgacgt
    13801 ccagttcaac atatcatttt tatttagtat ttaaatattt agcaagtctc ttcatacctt
    13861 cttttaaaac atccatttca taagcatagg aaatccttac aaatccttta ccgaattctg
    13921 taaaggatga gcctggaacg attgctaaat gtgttgattc aagtaaatcg acacaaaatt
    13981 cgaaatcgtc atcggtgata tgtttaatac ttgggaaaat ataaaacgcg ccttcaggtt
    14041 gagcggtaat ctcaaaacct aatttagtta attcagatac taaataattt cttcgttcta
    14101 cataagcttc gttcatatat ttaggagctt ctaatccttc gttaagtgct gttatacatg
    14161 ctatttgagc tggaacattg gcacaaatac aattataggc gtgcataaat gttaatttat
    14221 caatcaaata ttgaggtcct aatagaaaac caattcttat tccggttgct gagtgtgatt
    14281 tacttaaacc accaattaat attaattgat cacgaatgtc ttcaaattca gcgaaggata
    14341 catgtttacc actaaatgta ttttcagcat aaatctcatc gctaataata aataacggat
    14401 atttttttaa tacatttacg atatttaaaa cttcatttct ttttaaaact acgccagttg
    14461 gattagtcgg ataatttaac aagacagctt ttgtctttgg agaaatatga ctttctaatg
    14521 catcaggtgt aattttaaat tgtgttgctg ttgtatcaat ataaattggt ttaccaccta
    14581 gtacttcgat gagtggtatg tagcctgcat aaattggtcc cggtattata atttcatctc
    14641 caggctctat gatagaacgt aacgttgtgt ctattgcttc acttgctcca tttgtcacaa
    14701 taatttcttc aggatcatag gaaaaatgat aacgattttt gaagtattga ctaattgctt
    14761 cgcgagtttc taataaccct ttattgtgag agtatgatgt cttgtcattg ttaatagcat
    14821 caatatatgc ctttttcaca acatcaggca tcgggaagtc tggttggcca atagttaaat
    14881 taacacaatc atccaaatta ttcatacgat ttgaaaattg gcgaatactt ggtgctctta
    14941 aatatttaga attagaattt aaagaaagtt tcatttgtga cacctcaaat ataaatcaaa
    15001 tattgtctaa aaatttagaa attaataata tcataacata cttttaaagc aaaaaagggt
    15061 aaattactta agctttattt gaaaaacgaa aaatgtctaa atacactgta gtaactactt
    15121 ttcaaaatga ataaagtggt tacagttaat gtacttagac agtataaaat tatgaatctt
    15181 taaactgtga tggtctttgt aaattaatta ttggatttgt ccatttacaa acaaagtttg
    15241 tagataatac atatacgatg attacagata tacttattaa ataaagataa gtcattaatg
    15301 atataggatt atcgaatggg taccattcaa atcctctaac aatgccaata attaaaccat
    15361 gtaataaata tacgtatagc gtacgactac caatataagt atataatttt ttctttgttg
    15421 acattaaatt tagaaacgca gtcattgcga ttaatataat tccatataat ataagtcgtt
    15481 taaaaggact gaatatactc tgtccttcat tttcaagtga agtatatggt gaacttccca
    15541 ataaccaatc tgcattgata ggatgaatca cgtaaacgat aaaaaacaaa ataaaggtaa
    15601 tgatagatac tggtattagt tttttatttt taaaaatagc cgtatgtttt ttggtgaaaa
    15661 tgtaacctag ataaaatatt gggaaaaata cgattgtcct tgaaatgctt aagtagctat
    15721 cgatgttatc tgaaaaacct gctccaatag atataataat tgaaactgat agcactttat
    15781 atggattaaa tcttctaact attactaaaa tgacatgaaa gaaaaatagc gtgatcaaaa
    15841 accataacgc aaatactggg ttaaaaggat caagttgtaa ttcgtcactt ttacctgtta
    15901 agaaataata aattgaaaag aatgcaaaaa atatcatata aggtactatc aaacgttttg
    15961 aaattttttc taaatagtat ggtttatcaa tatttttcgc gaaataacca gatataaaca
    16021 aaaatgttgg catatggaaa ctataaataa ctaggtataa tgctgataaa tatttatcgc
    16081 cactagtgta gggttgtaac atatgtccaa atacgactaa taatattaaa attgctcttg
    16141 cgttgtcaaa aaaataatct ctctctttta atgaagtcat attttatctc ctttatgtcc
    16201 ataagactct tactaattta ttatattttg tattagttat gcaacaaatt tgtctattgt
    16261 gtcgaatagt attgtaaaaa ttataaaaaa agcatataat attataaata atatgtagtg
    16321 gagtagaaca acatgtataa acaacttgaa aaacttatta cactgactaa caatgactta
    16381 aacttagtga atagaagatt tggacaacgc acggatatca aatctgaaca gctagaactt
    16441 ctccgtattt tatttaatta cgatcgctta tcacagtatg atttgacgat gaagattagc
    16501 agggaacaat ctatagtttc aaggtggatt aagaaattag ttttgaaagg gtacatcaca
    16561 agtcaacaat ctagcgaaga tttaagatgt aaagaattaa ttttgactga tcaagcacgt
    16621 acattaattt cacaaataaa taatgcacgt tgcgaattga ttgaagcaag atgtcaatgt
    16681 ttatcggaag tcgaattaga caatttaaat caattacttg ataagttaaa tcaacgacgc
    16741 atatcgttgt aacacgacaa tagcaaacac ataatttagt gtcgttgtgc acacaacaaa
    16801 aattgaatca tgaattacaa gcaaaagtag cggtgattgt taaaattgat ggtaaacaat
    16861 caccgctatt tttgcttgtg tatgtataaa aaagggatca aaggtcatcc cccatgattg
    16921 atagtggggg atgacttttg atcctatgtt catgttgctt atttatattg tgggatgtcg
    16981 aagtatttgc cgacttcgcc aattttatca tagtagcctt tgatgatttt agcattgatg
    17041 ttagcccaat ctacatctgt agcatattgg tgtgttcctg gatgtgcagg attccatctc
    17101 attttgtaaa gtgtattttg accagctttt acatatgagt tgccgatgaa tttagcacca
    17161 ccaacgattg cttttgatac tgtgtcccaa ccagcttgtt tagcatattt aataccttca
    17221 cgtaaaggat cgttatcata tgcagcaata ccaaatacgt tatggtattt cgtgtttgag
    17281 ttagttacaa ctttgttgtt cactacatct gcacctttcg ctaattgaga agtaccgtta
    17341 cctgtttcta atagggcatg tgagataaga taaacttcat taatgccata catttgagca
    17401 gctttgttaa atgcagcacc ttggttttct aatacacctt taccttttaa gaattgatta
    17461 attttatcaa tagaaatatt ttgtggttgg tctaagcgta agaattgata ttttaatgct
    17521 ggatcttgag ctaaacgctt cgtatccatt gcatgcttaa catcattaaa gttagcatct
    17581 gtccacttac ctggtacacg ttgtacttgt ggtttatatt gtaaaccagc ttgtatttga
    17641 gcaacttggt ttaatgtcat acctgtttga ttatacttaa ttaattcttt agctaaatca
    17701 gttgatttaa tccatgctaa tttaccgtta gataatttac catagtacca agtttgtcca
    17761 ttaatgactt gttctttaac aactgcgaat ggttgttcat taaatgcttt taatgagtat
    17821 ttagctgtat cagaatttgg tgttacatag taataaccat taccattttt aattacataa
    17881 gtgtagttat aatctttggc agctgatgta gttggtttca cagcggttgg tgcagttaaa
    17941 tcttttgcat ttacccaacc agtgcggtta ttaatagtac cgtataaata aacatctttg
    18001 cctacagata cttgtttcgt tgcattaaat gtaccttgag caatgttatt gcctgttaaa
    18061 atgacttggt ttttagtacc ccaaggaacc attgataagc cgttatttga tttattaaca
    18121 gtatattttt gagtcgtttt aacttctttg cctaagtttt gaacatttaa gtcttttaca
    18181 ttgaaccaac ctaatgggat gttatggctt gtattgttta ataatacata cgtttcatta
    18241 ccatgagcac gctcttttgt tacatagaac gtacggtctg catatttcgc accgtttttc
    18301 gctgtttttt cataaacaga agcacgaata ccagtgttgt ttggtttaac ttgagcaatc
    18361 ttgctaactg tttgagtcgt ttgtggttta gtaacagtat aagcttttac agctgttttt
    18421 ggttgtgcta ctgctttttt aggtgcagca ggtacagcta aatatgcttt acttacccaa
    18481 ccagatttac catttacagt tccaaataaa tagatagatt tatcaatttg ttgttgctta
    18541 gtcgctttaa aagtttggtt acctgtacca gaaactgcac cagcttcttg tttataagtg
    18601 ccccaaggta ctgaatataa tttagtgcct ggttttactg tatatgtttg cattacattt
    18661 acaggtgatt ttgcattgtt ataaataacg tcaccttgtt taacccaacc aattaaagtt
    18721 ggactattgt aatctttaac taagtagaat ttgtttccac ctaaacttgc ttcttttgtt
    18781 acagcaaatg ttttttgaac ttctttcgtt ggcttaccag ttttgtcata aactgtagtg
    18841 aataagccat tgtttttagc attaatttga gcaacaccgt ttaatgatga aactgttaat
    18901 ttattatttg ttgtaggtgt tgatggctta ggtgttggtg taggcgtagg tttagcagta
    18961 tcaactaaat atgctttact tacccaacca gatttaccat tcacagagcc atataaataa
    19021 attgatttat caatttgttg ttgctttgaa gccttaaatg tttggtttcc agagccagac
    19081 acactaccag caacttgttt agatgtaccc caaggtactg tataaagttt cgtaccaggt
    19141 ttgattgaat atgattgatt tacatttaca ggtgatttag ctgtgttgta aaccacatcg
    19201 ccttctttaa cccaaccaaa tttattacca gaattgtaat cttgaacaag atagaatttt
    19261 tgattaccta atgtagctgt tttagataca gcaaatgttt tttgaacttc attagttgct
    19321 ttaccagttt tgtcgtatac agtagtatat aaaccactat ttgttggttt gatttgtgcg
    19381 acaccattgt ttgcagcaac tgttaattta ccagttgatg gtttcgacgg tgttgttggt
    19441 tttgatggtg tagtaggggt agttgtagat tgcgtacccc atggcgccac tttacccatt
    19501 tttattaaat atttttcatt aattaagtca tataattgat cataactata attatgactt
    19561 cttaaatatc catgtggatc ggcatggtca gtaccaccta aatatttact tacagcgtag
    19621 tgagtccata ctgtaccatt tccatcatac tcagcactgt ctggttttaa accataatat
    19681 tgtaattgtg tagctgcata gtcagcatag ttattcattg aacgtgcaaa tgaagcatag
    19741 tcgtgtgtgt gtacgatttc aacattgatg aatctagggt taccgactgc accgacaccc
    19801 caagataagt aatccgttgg tgctgtttcg attatacgat ccccatcaac aaatgcatgt
    19861 acgaatgcgt tttgatagtt atttttcata taactaattt caccatttat cgtcgaacga
    19921 tcattagctg tatcatgaac tacgatacct tcaggacgac ctacgccgtt acggtatgcg
    19981 tatttaggga agtaagatgt ataatcttct tcaattttag gtgcttttaa gttattttta
    20041 cgaatgtaat cgttaattga agagtttact tgtggtttat attttggcaa actcgttttt
    20101 ggtgttgcag caactgatct tggttgtgct gaagcgctaa aagtagttac tttaggtgtc
    20161 gcttcagttt tagctttagg tgctgatgta gttgcagctt taggtgctgc ggttttatat
    20221 tgcgtttcaa gagctgcagg tttagcagct gatttaatta attctggatt aatttgattt
    20281 tctgaattat catcttcatc atcaactaaa ctataaccag catttgtaac attagtgtta
    20341 gttttaggtg ctgtagtgct tgttgacttt gcaacaggct gcgtattatt tgtagtcgct
    20401 gattgattag cacgagtgtc accatttact tgtgcagtat caactttttg acttacttga
    20461 gcattgcctg ttttgttatt tgctgttttt ggttggacaa tagcagggtc ttgatatact
    20521 tgagtgccag aaatgttttg cgttggattt tttacctcag cttttgcttg ttcagtagtt
    20581 gctttaactt tattactatc taaaacgttt ttattagtag tttgatcttg tgtcgtctca
    20641 gctgcttgaa cttgatgtgc agtgactgct gaacctacaa gcgttaatgc aaccattgat
    20701 ggtagtttgt aattgaattt tttcgccatt ctatttatta ctcctaacat ttattaatta
    20761 ttactaacat tatagtacct gttttatata cctgtgcgta tttaaccaat ttgtaatata
    20821 ctcttaattt acattttcac tgtaaaaaaa tgaaaaaaat tttgaatctc aatggtgacg
    20881 tgacaaatca aatagaattg atgtcaattt aagaattggt ttattacaaa tgtttctaaa
    20941 atagatacaa ctttttttaa ttaagcgagg ttaacttttt tgtcatttca atatgctcga
    21001 tgccttcctc aagaaatata ttacctctca ttttaaagtt taaactttca taaaatggga
    21061 tagcatgaca ttgggcattc atagtagcta cgtaaaaacc ttcatcttta gctaatgatt
    21121 ctacagcttg cataagcatt ctacccattc cttgtccacg atgtgatttc atcacagcta
    21181 ctcgttctat tttgacagtt gtttcattaa tagggcgtat tcgagcagtg gcaactggct
    21241 gtccattatc atatccaatg aggtgaatag attcagattc atattcatca atttcacttt
    21301 cctcagggac gccttgttct tctacaaaca ctttctttct tatatagaag caatcttcta
    21361 acatcttttg attgtttact tttgaaaaca taaaattcct ccacatccta acaaaatcat
    21421 ttacatttaa tttactaata ataacataga ataacgattg ttattttcct caaaaataat
    21481 tagacataaa aaagctataa agatgattgt tgtgaatcat ctttatagct aattaattat
    21541 taatgttata gtgcttcttt tctaataatt gctgtatatt gccaaaagat tcgcatttga
    21601 gcaatattcc aattattcat tcccattgta aatcctgaag gcttaaatat attcacatca
    21661 gttacttcta aagctgctgc aactaaatat tgtaatggaa tgctaagtga tttcattgtt
    21721 tcggtaatgc cattttcatc atatacccat gaaaatggta actctgattc gaaaacatca
    21781 acactattga aaatttcagg tagggctaaa atataacatg caccatctac aataggattg
    21841 tcgatactat ttttgtaata aattaggagt gcttcatagt tttctcgatg ctcatgattg
    21901 acgtaaaaaa attcacttct aaaatgagcc atatcttggc tatgaatgat ttgttgttct
    21961 aacatattaa acatgccatt tatatttgca atcttttcgt atgtttttct actcatgtat
    22021 gatgctcctt tcataaatag taaatgttaa tcgtcatata atattaattt acaacaccat
    22081 tttggttatt tgaagcttgt ggcgcttgtt gtgtgccacc ttgattttga tttgagtttt
    22141 gatctgtagc aggttgttgt tgattgctgg aatcactgtt attagttgaa tcactgttct
    22201 cgttagatgt cttatcttta tctgtcgtat cattttgttc ttttctcaat aaactattat
    22261 ctaaaggcgt taatggtatt aatgaaccat aatgattaat gacacgttga tctaagaaat
    22321 catttttatc attaataggt gataattcta agtctttacg aagtaagttt gcatattttt
    22381 gaatgctttc aacacttgga tgataatagt aaataccatt taacatatca tctttacctt
    22441 ttaattgcgc agttttaatt tcaacatcat ttgttaagta catttttgct aaagctttaa
    22501 tttcagagtt agttaaatta tgctttgcat ttttacctac aatttgaatc acgttatcaa
    22561 gtttatcaat agaatcaact tcctgtgctt tttggaataa aatcttaatt aattccattt
    22621 gacgttgtcc acgttttaag tctgaatcat gatgtctagt tctagcaact gctaaagcct
    22681 catcaccatt taatttttgg tacccttttt taattttaat cttaccagta tcatctgtgt
    22741 taggttcatt taagtcgtat ggcacatcat agtatatgcc accaagctca tttacagcct
    22801 cgacaaatgc tttcatattg actctcacat aataatcaac aggtacattc atggtagctt
    22861 ctaccgaatc cattgcggca attggaccac catatgcatg tgcatgggta atcttatcgt
    22921 aatagccaac tttaggaatg tagctgatag tatcacgtgg aatactaagc attctaattt
    22981 gatgttttga ttgattaaaa gtagttaaaa tcatagcgtc tgatctagag tgttcagcat
    23041 cctgtccttt ttttcttctt ccatcgttat catcgatacc taagaaaaga atagagatag
    23101 gttgttcttc gggattgact ttattatctc ttaagttgga ttgacgatta gcatttttgc
    23161 tgtcttgaga agattcgaat gcatcttggg acgttttaaa aagtaacgta gcgaagacta
    23221 ttggaacaac aatgagaacc aatgctagaa ggatcaaaaa gtattttaaa aatttattca
    23281 tgattgatgc tcctatatta aatttttgtt aaatcataat cataatttta aaatataatt
    23341 agttgaacga tatttataca acttcattac taatacatta tagccttaaa ttgtagtact
    23401 ttatagtaaa aaatacaaat gttatgtcaa agttccttgt aatttgaatg ttttgttaaa
    23461 atcaaaaagc atttttaaaa atataatgcg actttagtat gatgtcttaa aaaatttaaa
    23521 ggcgtaatat aaattcgagt gatttataca aagtgataag gtataattaa ttttgttgta
    23581 tagataacat attagaaaaa cttattacta tgtagcgtaa agcataaatg aatgataagt
    23641 taatttttgt aagttgtatt attaggcatc taggttcaaa tttttaacga tttaaatata
    23701 taaaatgaat acattgtgag gcaattatca aatgaaattt aataaagtaa aactagttat
    23761 acatgcgtgt gtactattat ttatcattat ttctatagcg ttaatttttc atcgattaca
    23821 gacgaagaca cattctatag acccaataca taaggaaaca aaattatcag acaatgaaaa
    23881 atatttagtg gatcgtaata aggaaaaggt tgcgccgtct aaactaaaag aggtatataa
    23941 tagcaaggat cctaaatata agaaaattga caagtattta caaagttcat tatttaacgg
    24001 ttcagtagct atatatgaaa atggcaaatt gaaaatgagt aaaggttatg gatatcaaga
    24061 ttttgaaaaa ggtattaaaa acacaccgaa tacgatgttt ttaataggtt cagctcaaaa
    24121 attttcaaca gggttactgt taaaacagtt agaagaagaa cataaaataa atatcaatga
    24181 tccagtaagt aaataccttc catggtttaa aacatctaag cctatcccat tgaaagattt
    24241 aatgttgcat caaagtggat tatataaata taaatcctca aaagattata aaaatttaga
    24301 tcaagcagtt aaagcgattc aaaaacgtgg tattgatcct aagaaataca aaaagcatat
    24361 gtataacgat gggaattatt tagtacttgc gaaagtaatt gaagaagtta caggtaaatc
    24421 ttatgctgag aattattata caaaaatagg agatccttta aaacttcagc acacagcatt
    24481 ttatgatgaa caacctttta aaaaatatct agcaaaaggt tatgcttata atagtacagg
    24541 actttcattc ttaagaccta atattttgga ccaatactat ggtgcaggta atttatatat
    24601 gacaccaaca gatatgggta aattaattac tcaaatacaa caatataaat tattcagtcc
    24661 taaaataacc aatccattat tacatgagtt tggtacgaaa aagtatccag atgaatatcg
    24721 atatggtttc tatgctaagc caacattgaa tagacttaac gggggattct ttggacaagt
    24781 ctttactgtt tactataatg ataagtatgt agttgtactt gcattaaatg taaaaggaaa
    24841 caatgaagtt cgaatcaaac atatttataa tgatatttta aaacaaaata aaccttacaa
    24901 tacgaagggt gttattgttc aataattaat atagaagata taacatgtat atggcattaa
    24961 ggcatcgacc ttatctgacc agtatacgag ttatatcttc ttttttatag tggtaaaaag
    25021 tttaaagtat aaggttgaag aaggatgagt ttaaaaatat gtgttaactg ataaaagggg
    25081 aaatcatttg gtgagttggc atcagactaa aatgaatgaa gacgaatacg ttggtccatg
    25141 cgtggtgtga atgttttcta aatctaactg gaaatgataa tttaataatg tacacttttg
    25201 attgtttaaa catgtacaat ttttaaaaac ctagggataa ttttattgac gtgaacaaca
    25261 ttgagattat ctttagacat aaatatggat tttgaagtta ctatatttat tagttgtggt
    25321 tgttaatgta ttaattgaat tgattataat gattgtctta taagatatag aaggggactt
    25381 aaccatttaa aaattgaagg aaaataaaaa ccagctaaca aaagtgttag ctggttaaac
    25441 tttacattat taaaatgttg tttataagtg tgaagagtga ccgccttgca taacccaata
    25501 tgttccgaca acgaaacaaa gtgtaattac aagagcaaag ataactttga atgtttgtaa
    25561 acgtccatct ttaccttcag ttaaatgcat gaacattaat aattgaagtc ctgcttggac
    25621 gaatgcaaag ccaaagataa ttgtcaactt cgcgtggaat gttaatgacg tgtatagtgt
    25681 tacgtaaact gctaaaagcg ttaatacgat agatgcgata aatcctacag tatgtttcat
    25741 tattgtactc atccgctata caccatccct atcatatata cggcagtaaa gatgaaaacc
    25801 caaacaacat ctaagaagtg ccagtataaa cttactataa ataattttgg cgcattatat
    25861 ttgtctaatc cgcgtcgttg gatttggatt aataaacaaa tggcccaaac gatacctagc
    25921 gatacgtgac aaccatgcgt tcctaatagg ataaagaaac tagaccagta agaaccaatt
    25981 gttgggttaa cgccttctga tgcatagtgt gcgaattcat aaatttcgaa tccaacaaag
    26041 actaaaccta aaagtaacgt aatgatcatc caaaacatca ttaacttttg tttttcttgg
    26101 cgcatgtagt aaatagcaat accacatgtg taagaactga ataataatgc aaacgtcatt
    26161 attaaaacaa gaggcaattc aaataactca gtagtcattt tacctgcata atcgccacca
    26221 tgttgcaaag ttaatagtgt cgcaaatagg gtaccgaata acgcaaattc ggctgtaatg
    26281 aaaatccaaa agccaagctt atttaattcg ccttcatgtg tgcgtgaatc aatagtgttt
    26341 gtatcatgac tcatgactta cagcctccct ttctttaata cgcgcttctc ttaatctagc
    26401 ttcagtttct gcaacttcag cagcagggat atgatatccg tgatcgattt ggaaactgcg
    26461 ataaatcata gtaccaaaaa taccgaataa acaaattaat gctggaatta cagtttcgaa
    26521 aattaagaag aaaccgccga tagtcataaa gataccaatc cagaatccaa caggagtatt
    26581 gtttggcata tgaatgtctt tgtaattatg gttgtctaag taatgacgac catgttcttt
    26641 catatcaaca aatgtgtcgt agtcattcca atctggtgta atggcaaagt tgtatttagg
    26701 tggaattgct gatgcagtag tccactctag agtacgacca aggccatccc agttatctcc
    26761 agttgcttca cgtggagatt tgaagtgact gtatacgata ctaacaacaa ggaataagaa
    26821 tccgattgcc attaataatg caccgatagt tgaaattaag tttaataaga accaaccatc
    26881 tgatggcata taagtgtata aacgacgtgg cataccatct aatccaagaa tgaattgtgg
    26941 taagaaacaa acgttaaatc cgatcatgaa gaaccagaag caccatttgt ttaatgtttc
    27001 gtttaatttg taacccatca tctttggata ccagaagatt aaaccagcta agcaggcaaa
    27061 tacaacacca gtaaccaatg tatagtggaa gtgagctact aagaagtacg tattgtgata
    27121 ttgatagtca gctgatgcca ttgctaacat tacacccgta acaccaccta aaaggaagtt
    27181 agggataaat gctaatgaga atagcattgg tgactcaaat gtaatacgtc ctttatataa
    27241 tgttaataac cagttaaaca atttcacacc agttggaata ccaatcagca ttgttgaaat
    27301 tgagaagaat gagttgatta acgcaccatt acccattgtg aagaaatggt gaacccaaac
    27361 taagaaacta aggaacgcga tgccggcagt tgcccatacc atactttgat gtccgaataa
    27421 acgcttacga gcgaatgtcg ggataatttc tgagtaaata ccaaatgctg gaaggataac
    27481 gatataaact tcagggtgcc cccataccca gaagaagtta gcccaaagca ttggcatacc
    27541 gccatgtgca actgtgaaga atgctgtgtc aaatattcta tcagttgtca ttaatgctaa
    27601 cgctactgtt aaaggaggga aagcaagaat aacaattaat gtagtaataa atgttgttac
    27661 tgtaaacatt ggcatttgca taaacttcat agttggtgtt ttacatctta aaattgttac
    27721 aaagaagttg atacctgtag ctaaggtacc aagccctgaa atttgtatag ctattaagta
    27781 atagttaaca cccggaccag gactgaattc acctgctagt ggcgcatagt ttgtccaacc
    27841 agctgctggt gaaccaccaa taataaatga caggttgaat aaaatcatac ctgcaaagaa
    27901 tagccagaaa cttacgttgt ttaatactgg gaatgcaaca tcacgtgctc caatttgtaa
    27961 tggaacaacg atattccata aaccaaagat aaatggcatt gccatgaaga taatcatgat
    28021 tacaccatgt gtactaaaaa tttcgttata gtggttagat tctaaaaatt tgttatcagg
    28081 tactgttaat tgcgcacgaa taagtaacgc atcaatacca ccacggacga acattaatac
    28141 ggcacagatt aaatacataa taccgatttt cttatggtct acagatgtga accattcttt
    28201 gtaaagatat ttccataatt taaagtaagt aattactgcg attaaaccaa taactaagaa
    28261 tggggcacca atttgtgcca ttgtaatcat ccagttacct ttaactagta attgatccca
    28321 tggaaaattc attaatgtcc acctccatga tcatcattgt cttgatcttg cgcatctttt
    28381 gaaattttct tcatttcttt cgcatttttc gattcatctt tcttgaactc attgttatat
    28441 ggttcgtcat ttccaagaat catcaacttc ataccatgtc gtttatagtt cgcatttgta
    28501 atttgagctt tacgagcagg tattaatggt ttgtctgata catctttaaa catattttct
    28561 tcactagtga agtttggatc ttttaattcg aaattgaaac gtttatatgc atagaagatg
    28621 tattctggat cggctgctgg atcaacaaac gccatatgtg taccattaaa ttctaaagct
    28681 ttattaggtg tgcttggtaa taattgttta tcaaatgtat cttgatctaa cgttttctta
    28741 cctttaactt ctttcaccca tttgtcgtag tctttttgac taacggcatt tactttaaat
    28801 gtttgacgtg agaatccttc accattgaag ttagagttac gacctctgaa cgtaccagtt
    28861 tgagatgctt ctaacgtcca attcattgtc atgccagtca tggcatattt ttgaccacct
    28921 aattgtggaa tccagaaact tgtcattgta tccatagctt gaagcttaaa tacaacagga
    28981 cgatctttag ggattgttaa tgtattaaca gtctctatat gttcatctgg ataagcaaag
    29041 aaccatttgt atcctgcact tactgcatat acaaccattg gatctttctc actcttcggt
    29101 ggtttttcgt aatcgtataa agttttaact gtaggaatag ctaaagcagc aacgattatg
    29161 ataggtatta caaaccatat tgtttcaatg atggcattat ggtgcatctt accagattcg
    29221 gcattcttat tataactata cttgtaaata aaaatggcga acatgccaag tacaacgaaa
    29281 caaataacaa gcatgaagac gattgaataa agaatcaaga acttctgact acttgctact
    29341 ggcccttttg cgttgaaaat ttctatattt gaacaaccac taagtaaaat tagtgtgcca
    29401 aataatagaa gcaaagactt aaattttgac acttttttga cctcctaata ctacaaatgt
    29461 agggcttaac attaatttta agttattaca caatatttac aagggcttat gggaaaaaaa
    29521 ttaataaaat tgtatcaaaa atgttgataa atcaaggtgt gacgtgggtt cacacatttg
    29581 ttaaaattat gtgtacattt tgtgactaat agcgtttttg aaaaaattgc ttcatattta
    29641 aaaggaaagt tgagaatagc tttcaattaa aggttaattg aatgaaaata aaaaaagttt
    29701 gaaattgtct aataaattga aaatgatggc tagttgattg aaaggaagcg caaacatttt
    29761 aataaggaat ttgccaaaag taatttatcg ttaaacaatg aaaagtggaa atttgtttgt
    29821 tcaaaatcaa aacatttttt taaaaagagc agttaagttt taataatttg ataggtagtc
    29881 ctttggtata cagaaaaaat acaatatttg ggttaaagtt gcagatgaag ttagaataac
    29941 aaataaaatg gtagcaaaac atgttcgttt gctaccattt tatttgcagt atagtgatga
    30001 gggctattta acctcaatat acctagtgga aattttaatt gcaccatctg aatcagtgac
    30061 atgatattta atttgatatt taccagattt agatgtatca atttggccat cgactttaat
    30121 tttatcggtt aaatctccat cttctttatc aaatgcactt atgccgttta gaaggttata
    30181 atcttgacct ttcttaataa cgatatcatt agcgccttta atttgtggtg tagtattcgt
    30241 cgttgcatct gcatttaaat ttggtgttac tagtgtagca gaaacaccga gggctgataa
    30301 tgactgtaat agtttattca taatgtaacc tccgaatgat aagtcatttc tgagttagct
    30361 ttactatacc ttaatttata ttatttacac atcatactat agtcctaatc ttaccctata
    30421 aagaatgaaa tttaaatata aatttataaa taatgaggaa ataattatta ttttgaaaat
    30481 ctgccacaat tgtaaaatta ataatttagg gataatttga aatcaaatat aactttattc
    30541 gacagtttga acttaaattt ttgtgtatca aaacaataag aaaagggagg cgaaaatgat
    30601 aaagaaccac taatgattaa ctatgtagta gtactttatc atagtcacag cttctgtgtt
    30661 ggggcccatc cccaacttgc acattattgt aagctgactt ttcgtcagct tctttgttgg
    30721 ggccctgact agaattgaaa acttgttaca agcttatttt cattcaatta actactgtct
    30781 atataaaatt gttgagccaa gtacaaagtg aaatgctccc ttcaaagcct tcatttattc
    30841 aatgtctact ttgaagggag catttgatcg attaatgtcc cagatttttc ttgttattca
    30901 ttgcaattgc aaaataatat ttaatgtggg ttaaaagact ttattaaaac tcagttatta
    30961 gggagtggga cagaaatgat attttcg
//
LOCUS       DALAGD010000024        29917 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.024, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000024 DALAGD010000000
VERSION     DALAGD010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 29917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29917
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.024"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            223..861
                     /locus_tag="O2701_000854"
     CDS             223..861
                     /locus_tag="O2701_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HCZ4292760.1"
                     /translation="MYKINIIRSDSVYNNILKAPINDRDSIFTKEILVPFKKKFEVQH
                     MPIYNDDKQTMSAIQFLDAFQISPKDLRMSDQMSIQYLNNDFWSNCEKYLKVAIDQFS
                     NYSISSQVSNYHFTVLLGDRQKPLMYLNKNRGGDGGIPGYIMIYLVPSTSTINSMKSL
                     IAHEVNHNMRYQYIDWDGGSLIELIIAEGLAENYVESLYGKAHIGPWVTNTN"
     gene            880..1137
                     /locus_tag="O2701_000855"
     CDS             880..1137
                     /locus_tag="O2701_000855"
                     /inference="COORDINATES: protein motif:HMM:NF021537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292761.1"
                     /translation="MKNTIYNHLHLKHIFESMPYLYGDDINKLQGRPIVGLSHAAGYA
                     CGYHLVKYFLQKTNIPIEVATTLPAQKIINEVTEFWHTHTL"
     gene            1116..1856
                     /locus_tag="O2701_000856"
     CDS             1116..1856
                     /locus_tag="O2701_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCZ4292762.1"
                     /translation="MAYTYTLKDIIEITGVTKRTLHYYDEIGLLVPDKNDKNYRVYKQ
                     QDLEKLQKILILKSFDFDIAKIKQYISYDNEQLRKLLSEQISKLDKKISDLQLIRRSV
                     CEFIKGLSLIDTSILNKTLQSQYDKEASIKYGHTKAYQSFIRRKDSLQSQDIRHKLTT
                     IFNKFNHMSLSHYPIQDCSDLVFEWKAFMNTIADFDDETLCCIAKTYEDDTRFKDYFN
                     SYDNQNLASYISEAVNYFLSNVNKSDNF"
     gene            complement(2114..2767)
                     /gene="nreC"
                     /locus_tag="O2701_000857"
     CDS             complement(2114..2767)
                     /gene="nreC"
                     /locus_tag="O2701_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002464689.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation response
                     regulator NreC"
                     /protein_id="HCZ4292763.1"
                     /translation="MKIVIADDHAVVRTGFSMILNYQNDMEVVATAADGVEAYQKVME
                     YKPDVLLMDLSMPPGESGLIATSKIADSFPETKILILTMFDDEEYLFHVLRNGAKGYI
                     LKNAPDEQLLLAIRTVYKGETYVDMKLTTSLVNEFVSNSNQDTANTTDPFKILSKREL
                     EILPLIAKGYGNKEIAEKLFVSVKTVEAHKTHIMTKLGLKSKPELVEYALKKKLLEF"
     gene            complement(2790..3824)
                     /gene="nreB"
                     /locus_tag="O2701_000858"
     CDS             complement(2790..3824)
                     /gene="nreB"
                     /locus_tag="O2701_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501138.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation sensor histidine
                     kinase NreB"
                     /protein_id="HCZ4292764.1"
                     /translation="MINEDSIQLDTLLKKYYEHSIEKIVFADDNGKIIAMNDAAKDIL
                     SEEDNYSAVANAICHRCEGYTNAYDVQSCKDCFLESMQVQATNFQVFMKTKDQKVMPF
                     TATYQLIDQDRGIHAFTLQNVSSQIEQQEKLHQQRMMRKTISAQENERKRISRELHDS
                     VIQEMLNVDVQLRLLKYQEDTTKLLEDAENIEYIVAKLIDDIRNMSVELRPASLDDLG
                     LEAAFKSYFKQFEENYGIKIIYTSNIKNTRFDSDIETVVYRVVQEAILNALKYADVNE
                     INVGIRQTGRHLVAEVIDAGNGFDPSSKPKGSGLGLYGMNERAELVSGSVNIETKIGE
                     GTNVTLNIPI"
     gene            complement(3848..4300)
                     /gene="nreA"
                     /locus_tag="O2701_000859"
     CDS             complement(3848..4300)
                     /gene="nreA"
                     /locus_tag="O2701_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation accessory nitrate
                     sensor NreA"
                     /protein_id="HCZ4292765.1"
                     /translation="MTPEAIIEDRRFQETLDKIRKEEGYDFAAIAFYESNKPSSPIKW
                     HYVSGNKNNRFKLIILRKGRGLAGTVMKTGKRMVIANVGLALGPEEKIDYPILLSESL
                     TAVLAVPLWYKNQVYGVLLFGQRDGRPLPKIFDNDDIQRKFGIFNDDK"
     gene            complement(4320..4997)
                     /gene="narI"
                     /locus_tag="O2701_000860"
     CDS             complement(4320..4997)
                     /gene="narI"
                     /locus_tag="O2701_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCZ4292766.1"
                     /translation="MLNQFLWVIFPYLCLVVFVAGHIARYRYDKFSWTAKSSELIERK
                     RLMWGSLLFHLGIIPVFFGHVVGLLIPKPWMDAVGVSEHLYHIGAVYIGSIFGIITLI
                     GMFLLTARRVTTKSIRRLSSASDIFVNFLLLLIVFMGCYATLVTNIQHPDFNYRTSLS
                     IWFRQLFMLKPDASLMSGVPIAFKMHILLGFTIMACWPFTRLVHVWSVPLTYINRRYI
                     IYRKNKV"
     gene            complement(4990..5565)
                     /gene="narJ"
                     /locus_tag="O2701_000861"
     CDS             complement(4990..5565)
                     /gene="narJ"
                     /locus_tag="O2701_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCZ4292767.1"
                     /translation="MINFDNFKKYQESFGYMAQQLCFPEKLTFHPKTFEETISKDHPA
                     YEDLVAFRNVMMTFSLSEIKAIYTDTFDFTKKAPLYMTYNKFDTQKERGQMLAKLKVL
                     YEMFGLEMVDNELSDYLPLMLQFLQVAEWRNDPRAEENIQLIIMIIEDGTYVMANALG
                     EDNNPYAYVIQALRKTLKLCLTSPKGVKHHA"
     gene            complement(5558..7117)
                     /gene="narH"
                     /locus_tag="O2701_000862"
     CDS             complement(5558..7117)
                     /gene="narH"
                     /locus_tag="O2701_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCZ4292768.1"
                     /translation="MKIKAQVAMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNV
                     ETKPGVGYPKRWEDQEHYKGGWVLNRKGKLELKSGSRISKIALGKIFYNPDMPLIKDY
                     YEPWNYNYEHLTTAKSGKHSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPN
                     IQKIEEDIKFQFDETFMMYLPRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACR
                     GWRYCMTGCPYKKVYFNWKTNKAEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYD
                     ADRVHEAASAVDEKDLYEKQLDIFLNPFDEEVIAQAEKDGIGYDWIEAAQNSPIYKLA
                     IEYKLAFPLHPEFRTMPMVWYCPPLSPIMSYFEGKNTTQNPDAIFPAIEEMRLPIEYL
                     ANIFTAGDTEPVKGALQRMAMMRSYMRSQVTQQPFDTSRLERLGITERQTKDMYRLLG
                     LAKYEDRFVIPTSHKETYLDTYHAQGSTGYNYGGEHFGDNCEGCGVAVGSGKTGQEIY
                     NENFYGGIFRD"
     gene            complement(7107..10796)
                     /locus_tag="O2701_000863"
     CDS             complement(7107..10796)
                     /locus_tag="O2701_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCZ4292769.1"
                     /translation="MGKFGLNFFKPTEKFNGNWSILESKSREWEKMYRERWSHDKEVR
                     TTHGVNCTGSCSWKVFVKNGVITWENQQTDYPSCGPDMPEYEPRGCPRGASFSWYEYS
                     PLRIKYPYIRGKLWDLWTEALEENNGNRVAAWASIVENEDKAKQYKQARGMGGHVRSN
                     WKDVTEIIAAQLLYTIKKYGPDRIAGFTPIPAMSMISYAAGARFINLLGGEMLSFYDW
                     YADLPPASPQIWGEQTDVPESSDWYNASYIIMWGSNVPLTRTPDAHFMTEVRYKGTKV
                     ISVAPDYAENVKFADNWLAPNPGSDAAIAQAMTHVILQEHYVNQPNERFINYAKQYTD
                     MPFLIMLDEDENGYKAGRFLRASDLGQTTEQGEWKPVIHDAISDSLVVPNGTMGQRWE
                     EGKKWNLKLETEDGSKINPTLSMTEGGYELETIQFPYFDSDGDGIFNRPIPTRQVTLA
                     NGDKVRIATIFDLMASQYGVRRFDHKLESKGYDDAESKYTPAWQEAISGVKQSVVIQV
                     AKEFAQNAIDTEGRSMIIMGAGINHWFNSDTIYRSILNLVMLCGCQGVNGGGWAHYVG
                     QEKCRPIEGWSTVAFAKDWQGPPRLQNGTSWFYFATDQWKYEESNVDRLKSPLAKTEE
                     LKHQHPADYNVLAARLGWLPSYPQFNKNSLLFAEEAKDEGIDSNEAILQRAIDEVKSK
                     QTQFAIEDPDLKKNHPKSLFIWRSNLISSSAKGQEYFMKHLLGTKSGLLATPNEDEKP
                     EEITWREETTGKLDLVVSLDFRMTATPLYSDIVLPAATWYEKHDLSSTDMHPYVHPFN
                     PAIDPLWESRSDWDIYKTLAKAFSEMAKDYLPGTFKDVVTTPLSHDTKQEISTPYGVV
                     KDWSKGEIEAVPGRTMPNFAIVERDYTKIYDKYVTLGPVLEKGKVGAHGVSFGVSEQY
                     EELKSMLGTWSDTNDDSVRANRPRIDTARNVADAILSISSATNGKLSQKSYEDLEEQT
                     GMPLKDISSERAAEKISFLNITSQPREVIPTAVFPGSNKQGRRYSPFTTNIERLVPFR
                     TLTGRQSYYVDHEVFQQFGESLPVYKPTLPPMVFGNRDKKIKGGTDALVLRYLTPHGK
                     WNIHSMYQDNKHMLTLFRGGPTVWISNEDAEKHDIQDNDWLEVYNRNGVVTARAVISH
                     RMPKGTMFMYHAQDKHIQTPGSEITDTRGGSHNAPTRIHLKPTQLVGGYAQISYHFNY
                     YGPIGNQRDLYVAVRKMKEVNWLED"
     gene            complement(11105..12082)
                     /gene="cobA"
                     /locus_tag="O2701_000864"
     CDS             complement(11105..12082)
                     /gene="cobA"
                     /locus_tag="O2701_000864"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCZ4292770.1"
                     /translation="MSVEEYGKVYLIGAGPGNPNYLTKKAERLIREADVILYDRLVNP
                     LILQYANLTTEIIDVGKKPYAKHIQQEKINDCIVEAARRYNKVVRLKGGDPAIFGRVQ
                     EEVDTLNEHHIAFEIVPGVTSASAAVATMQTGLTMRTVAKSVTFSTGHFKDSEENEVD
                     VNSLVNGGTLAIYMGVKRLGKIITQIQQYTDIDYPIAIVFQASCFNEFVVKGRLSNII
                     SKLQHYSIEAKPGICIIGEVVDYTENTPKSYDPMKQFYVVSGSKHDALMLCEHLYDEG
                     YGCLLNPNDTSNGTYHSSQYDYYDAFIKQQENVTYISTDRADANTVLCH"
     gene            complement(12073..12387)
                     /gene="nirD"
                     /locus_tag="O2701_000865"
     CDS             complement(12073..12387)
                     /gene="nirD"
                     /locus_tag="O2701_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCZ4292771.1"
                     /translation="METKEKIKVTTIDELTPLIGKKVIVKGKEVGLFLTESGKIHAIH
                     NICPHKQGPLSEGTVSGEYVFCPLHDQKIDLNTGIVQEPDEGCVDVYEVEVTDGNVYI
                     CL"
     gene            complement(12391..14796)
                     /locus_tag="O2701_000866"
     CDS             complement(12391..14796)
                     /locus_tag="O2701_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCZ4292772.1"
                     /translation="MAKQKLVMIGNGMAGIRTIEEILERANDLYDITVIGKEPYPNYN
                     RIMLSNILQNKMTVEETIMNPYEWYEEHGIELITNDPVIEVDRANQSVTTANGIEVSY
                     DKLIFATGSKAFVIPVPGSTLPSVIGWRTLDDTEQMMNIAKTKKKAIVIGGGLLGLEC
                     ARGLLDQGMEVTVLHLAEWLMEMQLDRKAGNMLKADLEKQGMKFEMQANTTEILGEDD
                     VEGVKLADGREIPADLVVMAVGIRPYTEVAKESGLDVNRGIVVNDVMQTSDSNVYAVG
                     ECAEHNGKVYGLVAPLYEQGKVLADHLTNKETNGYKGSTTFTSLKVSGCDLYSAGQIV
                     ENAEIKGIEIFNSVDNNYKKIFLKDGNVVGAVLYGDIDDGSRFYNMMKKGESTEDYTL
                     VSLLTKGGEEASLSIADMADDETICGCNGVDKGTIVNAITENGFTTVEEVTAKTKAGN
                     SCGKCKPQIAQILQHTLGDDFVAAKPAGICGCTDLTRDQIVTQIRAKGLKTSKEVRHV
                     LNFKNKGGCPKCRPAINYYLNMVYPHDHEDERESRFANERYHANIQNDGTFSVIPQMR
                     GGVTDADQLIRLGEVAKKYHVPLVKVTGSQRVGLYGVKKEELPNIWEDLGMRSASAYG
                     KKTRSVKSCVGKEFCRFGTQYTTRLGIRLEKTFEYIDTPHKFKMGVSGCPRSCVESGV
                     KDFGIISVENGFQIYIGGNGGTEVEKAEFLTTVETEDEVIKLCGALMQYYRETGIYAE
                     RTAPWLRRLGFENVKEVLLDPERQNELFERIMDAKKAVEAEPWEAITSNAQARRIFEV
                     EKV"
     gene            complement(14862..15593)
                     /locus_tag="O2701_000867"
     CDS             complement(14862..15593)
                     /locus_tag="O2701_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HCZ4292773.1"
                     /translation="MNGNIIVAHGMRHGRQNQALEAFISELVKDDIHHYDIAFLESEH
                     QDLETVMTTLIQSGVDHFKIVPLLIFSAMHYLKDIPNIVHEMKRRYPDIKVEVSEPLG
                     THPLMRRLIEQRICDALAGDEQQVGVMVVAHGNINGKFTKAHEELQICAQNLQISKPT
                     YARTLYGEISFTHDLESISKRYQKLIVVPLFLYDGRLVNKVKQQMNDMVINTDIHFTP
                     SINFDPILKQIINDRLESLMIPMKI"
     gene            complement(15823..16359)
                     /locus_tag="O2701_000868"
     CDS             complement(15823..16359)
                     /locus_tag="O2701_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4292774.1"
                     /translation="MTKTMIRLASEQDAEKLQQLMHEAFTPLRELGIDWPSVNANLDA
                     VKENIDKNTTFVMTIDDEIISTITVRYPWGSVKSISGYPFVWWFATNPEYEGKGYGSQ
                     LLTYVEEAFLRDTLKSAAVTLGTSARLHPWLLKIYEKRGYEIYSEHENDDGDLGVIMR
                     KVLIPERFDETILGQPPF"
     gene            complement(16592..17416)
                     /locus_tag="O2701_000869"
     CDS             complement(16592..17416)
                     /locus_tag="O2701_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCZ4292775.1"
                     /translation="MIENKKTVEDTYSTGAIVDSISSSVQMKQVMVQQTPGRYMLKAM
                     MAGFLLSIVTVFMFGIKTQFASTHVDGLINLMGAIAFSLGLILVVLTNSELLTSNFMY
                     FTVGWYYKVVSVKKMTWILLYCFLGNILGGFVLFFLMKFAHVMTPEMTQALTALVQKK
                     TVDSTWLNIFTKGIFCNFFINIGIFISMQFKGGLTKAFFIACGVVVFVYMGYEHVVFN
                     AGLYAGMVFFNLDAVSWLHVLKNIVFAFLGNFVGGGIFVGLVYAFLNGKRNRLEQQ"
     gene            complement(17633..17815)
                     /locus_tag="O2701_000870"
     CDS             complement(17633..17815)
                     /locus_tag="O2701_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292776.1"
                     /translation="MVERYIKVLILYIFTTLLSSISVTSKCVPNKVIRFILRTAIGYS
                     VFAYGLHYFSNLKKNK"
     gene            complement(17899..18366)
                     /locus_tag="O2701_000871"
     CDS             complement(17899..18366)
                     /locus_tag="O2701_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HCZ4292777.1"
                     /translation="MTIKVEDNKIIFSRTIEAPIEKVFDAYTTKALFEKWFHPKDAST
                     KVFRFNAVSGGDAFYAIKTPTMTSYTLAEYKTVKRPYLIEYIDSFATPQGAKDTKMPS
                     MKITLSFSKSNTTKTTVTSTSVFPTKEAAQQVINMGVEQGMNQTLDQLDALLK"
     gene            complement(18552..20102)
                     /gene="adcA"
                     /locus_tag="O2701_000872"
     CDS             complement(18552..20102)
                     /gene="adcA"
                     /locus_tag="O2701_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HCZ4292778.1"
                     /translation="MKKKLGMLLLVPAVTLSLAACGNDDGKDKDGKVTIKTTVYPLQS
                     FAEQIGGKHVKVSSIYPAGTDLHSYEPTQKDILSASKSDLFMYTGDNLDPVAKKVAST
                     IKDKDKKLSLEDKLDKAKLLTDQHEHGEEHEHEGHDHEKEEHHHHHGGYDPHVWLDPK
                     INQTFAKEIKDELVKKDPKHKDDYEKNYKKLNDDLKKIDNDMKQVTKDKQGNAVFISH
                     ESIGYLADCYGFVQKGIQNMNAEDPSQKELTKIVKEIRDSNAKYILYEDNVANKVTET
                     IRKETDAKPLKFYNMESLNKEQQKKDNITYQSLMKSNIENIGKALDSGVKVKDDKAES
                     KHDKAISDGYFKDEQVKDRELSDYAGEWQSVYPYLKDGTLDEVMEHKAENDPKKSAKD
                     LKAYYDKGYKTDITNIDIKGNEITFTKDGKKHTGKYEYNGKKTLKYPKGNRGVRFMFK
                     LVDGNDKDLPKFIQFSDHNIAPKKAEHFHIFMGNDNDALLKEMDNWPTYYPSKLNKDQ
                     IKEEMLAH"
     gene            complement(20291..20362)
                     /locus_tag="O2701_000873"
                     /pseudo
     CDS             complement(20291..20362)
                     /locus_tag="O2701_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20397..20663)
                     /locus_tag="O2701_000874"
     CDS             complement(20397..20663)
                     /locus_tag="O2701_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCZ4292779.1"
                     /translation="MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALE
                     GIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIKIASCKYHY"
     gene            complement(20663..20914)
                     /locus_tag="O2701_000875"
     CDS             complement(20663..20914)
                     /locus_tag="O2701_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCZ4292780.1"
                     /translation="MIIKNYSYARQNLKALMTKVNDDSVMVTVTSTDDKNVVIMSESD
                     YNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKDIDV"
     gene            complement(21079..21180)
                     /locus_tag="O2701_000876"
     CDS             complement(21079..21180)
                     /locus_tag="O2701_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292781.1"
                     /translation="MLRSVSDTNLLGQYEKAISISDMIEIVFLMYEV"
     gene            complement(21187..21786)
                     /locus_tag="O2701_000877"
     CDS             complement(21187..21786)
                     /locus_tag="O2701_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCZ4292782.1"
                     /translation="MTKKLLTLFIVSMLILTACGKKESATTSSKNGKPLVVVYGDYKC
                     PYCKELDEKVMPKLRKNYIDNHKVEYQFVNLAFLGKDSIVGSRASHAVLMYAPKSFLD
                     FQKQLFAAQQDENKEWLTKELLDKHIKQLHLDKETENKIIKDYKTKDSKSWKAAEKDK
                     KIAKDNHIKTTPTAFINGEKVEDPYDYESYEKLLKDKIK"
     gene            complement(21805..22164)
                     /locus_tag="O2701_000878"
     CDS             complement(21805..22164)
                     /locus_tag="O2701_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HCZ4292783.1"
                     /translation="MKRFVATVLLLLVFISGCGNDKYVKEIDEAVKIQNQKQEQLAKK
                     GNGDRVDHFERKDANIYVYDKDKIIILAYKPLSNDEVHYYAYDFSDKRVSYKQDFDSR
                     RYYQQHDADYHEENMTN"
     gene            22419..23669
                     /gene="fmhA"
                     /locus_tag="O2701_000879"
     CDS             22419..23669
                     /gene="fmhA"
                     /locus_tag="O2701_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhA"
                     /protein_id="HCZ4292784.1"
                     /translation="MNFVTLTSDEFNAFTTKHFSHYTQSAIHYNHRVDLKGDVHLVGV
                     KDDNGQVIAGCLLTEARTLKFFKYFYTHRGPVMDYTNQSLVAFFFKALTSYLKKHNCL
                     YVLVDPYLIENLRNADGEIVKSYDNRAFVRTMDKLGYKHQGFPVGYDSMSQIRWLSVL
                     DLKDKTEDQLLKEMDYQTRRNIKKTYDIGVKTKTLTIDETQTFFDLFHMAEEKHGFKF
                     RELPYFEEMQKLYDDHAMLKLAYIDLNEYLKTLQLKQQQLTAELSGVEEALEESPNSK
                     KNKTKRTQLEQQLNSNKRKIDNTIEQIEQDGAVLNLASALFIYNEHEVYYLSSGSNPK
                     YNAYMGAYHLQWEMIKFAKAHHIDRYNFYGITGDFSESSEDYGVQQFKKGFNAHVEEY
                     IGDFIKPIKLLLYKVQTYLNHKRR"
     gene            complement(23766..24497)
                     /locus_tag="O2701_000880"
     CDS             complement(23766..24497)
                     /locus_tag="O2701_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCZ4292785.1"
                     /translation="MIQLNNIHKSFNDVEVIKGIDLSVEQGEVVTLIGRSGSGKTTLL
                     RMINALEIPTEGTVYVNGKTYTSKDKKSQIEVRKQSGMVFQSYNLFPHKTALENVMEG
                     LITVKKLKKDEARGKSLELLEKVGLTHVKDQRPHALSGGQQQRVAIARALAMNPKVML
                     FDEPTSALDPELVNDVLKVIKDLANEGMTMVIVTHEMRFAKEVSNNIVFIHEGMIGEQ
                     GAPEEMFNRPKTEELRRFLNVINEE"
     gene            complement(24494..25213)
                     /locus_tag="O2701_000881"
     CDS             complement(24494..25213)
                     /locus_tag="O2701_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCZ4292786.1"
                     /translation="MFLNLNSEQQHALDAAKQAFGPMLEGLVKYSIPITLVTFVLGLI
                     IALFTALMRISTSKILRSISRVYVSIIRGTPMIVQLFIIFYGIPELGRLLTNDADNQW
                     TLAPVVAAIIGLSLNVGAYASEIIRGGIISIPKGQTEAAYSIGMTYGQTIQRIILPQA
                     IRVSIPALGNTFLSLIKDTSLLGFILVAEMFRKAQEVASTTYEYLTIYVLVALMYWVV
                     CFIISIIQGIYESYIERGYRS"
     gene            complement(25194..25973)
                     /locus_tag="O2701_000882"
     CDS             complement(25194..25973)
                     /locus_tag="O2701_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCZ4292787.1"
                     /translation="MKRLLFVMIAFVFILAACGNNSSKDKEASKDSKTINVGTEGTYA
                     PFSFHDKDGKLTGYDIDVIKAVAKEEGLKLKFNETSWDSMFAGLDAGRFDVIANQVGI
                     NPDREKKYKFSKPYTFSSAVLVIRENEKDIKDFDDVKGKKLAQTFTSNYGKLAKDKGA
                     DITKVDGFNQSMDLLLSKRVDGTFNDSLSYLDYKKQKPNAKIKAIKGNAEQSRSAFAF
                     SKKADDETVQKFNDGLKKIEENGELAKIGKKWFGQDVSKSK"
     gene            complement(26094..27533)
                     /gene="mdeA"
                     /locus_tag="O2701_000883"
     CDS             complement(26094..27533)
                     /gene="mdeA"
                     /locus_tag="O2701_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdeA"
                     /protein_id="HCZ4292788.1"
                     /translation="MSKKQKLTMIITMLMGGFFGLLNETLLVTALPSIMKDFEISYTQ
                     VQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGTLLGGLSPHFATLLVA
                     RIIQALGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYFN
                     WRSLFHVVAPIAAVTFLIGFKTIKNVGTTIKVPIDFISVIFSVLGFGGLLYGTSSISE
                     KGFDNPIVLVSMIGGVVLVALFVLRQYRLSTPLLNFAVFKNKQFTVGIIIMGVTMVSM
                     IGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYEKFGPRNLALVGMA
                     IVVITTAYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSM
                     TNTVQQIAGSIGTAALITILSHASKNFSPTMSDYNGMNKIDMMNQIKVDTMLHGYHAG
                     FLFALLITVVSFFCSFMLQGKKKEVDSRQ"
     gene            27707..27811
                     /locus_tag="O2701_000884"
     CDS             27707..27811
                     /locus_tag="O2701_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCZ4292789.1"
                     /translation="MKLDLQTARRNLNSPNIKTRKRALKIIKQHKRAK"
     gene            complement(27965..28651)
                     /locus_tag="O2701_000885"
     CDS             complement(27965..28651)
                     /locus_tag="O2701_000885"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCZ4292790.1"
                     /translation="MPKLILCRHGQSEWNAKNLFTGWEDVNLSEQGINEATRAGEKVR
                     ENNIAIDVAFTSLLTRALDTTHYILTESKQQWIPVYKSWRLNERHYGGLQGLNKDDAR
                     KEFGEEQVHIWRRSYDVKPPAETEEQREAYLADRRYNHLDKRMMPYSESLKDTLVRVI
                     PFWTDHISQYLLDGQTVLVSAHGNSIRALIKYLEDVSDEDIINYEIKTGAPLVYELTD
                     DLEVIDKYYL"
     gene            complement(28978..29844)
                     /locus_tag="O2701_000886"
     CDS             complement(28978..29844)
                     /locus_tag="O2701_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCZ4292791.1"
                     /translation="MSHNENLKLAQRGAYLSLIVYIILSIVKYVTGFVFNSAAVRADA
                     LNNMTDIIVSLAVIIGLKISIKPADRNHPYGHLKSENISSLLVSFVIMFVGIQVVIQN
                     APRLFKEDDVVPNAITIIVSLISGLVMLIVFAVNQRLAKRTKSSSLNSAAKDNLSDSL
                     VSIGTAIGLIFTQIGFPIVDIILATLLGLLIVYTGFGIFKEAIFMLSDGFNETELEAY
                     RNDILEVDEVQEVKSIKGRYHGSSVFIDVTIVVDANLSLVEAHQICDNVEHHLHKKGI
                     SSVYVHPEPDHL"
BASE COUNT         9410 a         5810 c         4243 g        10454 t
ORIGIN      
        1 tgattgtgaa aaagtgaaca taaggatttt cccttattta tgaagtgatt taccctatac
       61 aatgtccctt taagatgctt ttagacactt attcgccatc attttaatca tattatttag
      121 gaacactcac atacttttct taattttttt caattagtta caattactgc ttgaaaatca
      181 cgttacgtta tcctttatgc tgacaacata aggaggttta tcatgtataa aatcaatatt
      241 attagatcag attccgtcta taataatata ctcaaggcac caataaacga ccgtgattca
      301 attttcacta aagaaatatt agtacctttt aaaaagaaat ttgaggtaca acatatgcct
      361 atttataatg atgacaaaca gacaatgagt gcgattcaat ttttagatgc ctttcaaatt
      421 tctcccaaag atttacgcat gtctgaccaa atgtcaatac aatacctgaa taatgatttc
      481 tggagtaatt gcgaaaaata tttaaaagtt gctatagatc aattttcaaa ttatagtatc
      541 tcatctcaag tttctaacta tcatttcaca gtattacttg gagatcgcca aaaaccactt
      601 atgtatctaa ataaaaatcg cggtggtgat ggtggcatac caggttatat tatgatttat
      661 ttagtgccga gtacaagtac aattaattct atgaaaagct taattgcaca tgaagtaaat
      721 cataatatgc gctatcaata tattgattgg gatggcggaa gtttgattga attgattatt
      781 gcagaaggat tagctgaaaa ctatgtagaa tcattgtatg gtaaagcgca tattggaccg
      841 tgggtaacaa atactaattg aagtcgtgat aacgtaaaaa ttaaaaatac tatttataat
      901 catttacatt taaagcatat atttgaatcg atgccttatc tctatggtga tgatattaat
      961 aaacttcaag gtaggcctat cgttggctta tctcatgctg ccgggtatgc atgtggctat
     1021 cacttggtaa aatacttttt acaaaaaaca aacataccta ttgaagttgc tacaacactt
     1081 ccagcacaaa aaataataaa tgaggtgact gaattttggc atacacatac actttaaaag
     1141 atattattga aattacaggt gtaactaaaa gaactttaca ttattacgat gaaataggat
     1201 tattagttcc agataaaaat gataaaaatt atcgcgttta taaacagcaa gacttagaaa
     1261 aattacaaaa gattttaata ctcaagtctt ttgattttga tatcgctaaa ataaaacaat
     1321 acatttcgta tgataatgaa caattgcgaa agttattatc agagcaaata agcaagttag
     1381 ataaaaagat ttctgactta caattaatta gacgctctgt atgtgaattt attaaaggac
     1441 tctctctaat agataccagc attttaaaca agacactaca gtcacaatat gataaagaag
     1501 catctataaa atatggtcat acgaaagcat atcaatcatt tattagacgt aaagacagct
     1561 tacaatcgca ggatatcaga cataaattga caactatctt caataaattt aatcatatgt
     1621 ctttgagtca ttatccaatc caagattgta gtgatctcgt atttgagtgg aaggcattta
     1681 tgaacactat cgctgatttt gatgatgaaa cattatgctg tattgctaaa acatatgaag
     1741 atgatacgcg tttcaaagat tactttaatt catatgataa tcaaaattta gcatcataca
     1801 tttcagaagc tgttaattat tttttgagca atgtgaataa gagcgacaat ttttaatcct
     1861 catacagatg ctacaacact gaatacacca actaaaataa ttgttcaaag tagaaaacta
     1921 atcatttatt ctctaaatca ataactatat taaaagttat acctttgcaa agcgaattag
     1981 tataggttac cgaaaggaga aaggattagg ttccattcga tttataaaaa aatatatttc
     2041 gaaacataca aaagcgccag aagataatca tttcctctcg cgcttttata ctgaattata
     2101 ttaatttcaa actctaaaac tctaataatt tctttttcaa tgcatattca actagctcag
     2161 gtttactctt taagccaagc tttgtcataa tatgcgtctt atgtgcttct actgttttca
     2221 cagatacaaa taatttctct gcaatttctt tattcccgta acctttggca ataagtggca
     2281 atatttctag ttctcgtttt gataagattt taaaaggatc tgttgtgttt gcagtgtcct
     2341 gatttgaatt agatacaaat tcattcacta aagatgttgt aagtttcata tctacatatg
     2401 tttcaccttt atatacagtt cgaatagcta acaataattg ttcatcaggt gcatttttca
     2461 atatgtaacc tttcgcacca ttacgcaaca catggaacaa atactcctca tcatcaaaca
     2521 ttgttaatat tagtatttta gtttcaggaa aactgtcagc aattttactc gtagcgataa
     2581 gacctgactc acctggtggc atacttaaat ccattagtaa cacatcaggt ttatattcca
     2641 ttactttttg gtaagcttcg acgccatctg cagccgttgc aacaacttcc atatcatttt
     2701 gataatttaa aatcatagag aaccccgtac ggacaacagc gtgatcatcg gcaatgacta
     2761 ttttcaattt tattccccca atgtatgttt caaattggaa tgttcaatgt aacattggta
     2821 ccctcaccaa ttttcgtttc aatattgacg ctaccgctga ctaactcagc tcgctcattc
     2881 attccatata aaccgagtcc agaaccttta ggcttagaac ttggatcaaa accatttccc
     2941 gcatctatca cttctgctac caaatggcgc ccagtttgac ggatacctac atttatttca
     3001 tttacatcag cgtatttcaa cgcatttaaa atagcttctt gcactactcg ataaacaacc
     3061 gtttcaatat cactatcaaa gcgagtattt ttaatatttg atgtatatat gatttttatt
     3121 ccataatttt cttcaaactg tttaaaatat gatttaaaag ctgcttcaag gcctagatca
     3181 tccaaagaag cgggtcttaa ttcaaccgac atattacgta tatcatcaat taatttagcg
     3241 acaatatatt caatattttc tgcgtcttcc aaaagcttag ttgtatcttc ttgatatttt
     3301 aataatctca attgaacatc tacattgagc atttcttgaa tcacactatc atgtaactct
     3361 ctagaaattc gctttctttc attttcttgg gctgagattg ttttacgcat catacgttgt
     3421 tgatgcaatt tctcttgctg ttcaatttgt gatgaaacat tttgaagcgt aaatgcatga
     3481 attcccctgt cttgatcaat caactgatat gttgctgtaa atggcatcac tttttgatct
     3541 ttcgtcttca taaatacttg gaaattcgta gcttgtactt gcatcgattc taagaaacag
     3601 tctttacatg attgtacatc ataggcattt gtataccctt cacatctgtg acaaatcgca
     3661 ttcgccacag cactgtaatt atcttcttca gataatatat ctttagcagc atcattcatt
     3721 gcaataattt taccgttatc atcagcaaaa actatctttt cgattgaatg ctcataatat
     3781 tttttcaata aagtatctaa ctgtatactg tcctcattaa tcatgactta caccctaatt
     3841 catctcatta tttatcatca ttgaaaatac caaacttacg ttgaatatca tcattatcaa
     3901 atatttttgg taaaggacga ccatctcttt gaccaaataa tagtacgcca tacacttgat
     3961 tcttatacca aagcggcact gctaaaactg ctgttaatga ttcgctcaat aaaattggat
     4021 agtcaatctt ttcttcaggc cctaaagcta aaccaacatt ggctattacc atacgctttc
     4081 ctgttttcat aacagttcca gctaatccac gaccttttct taaaataatc aatttaaatc
     4141 gattattttt attacctgaa acatagtgcc attttattgg agatgatggt ttgttagatt
     4201 catagaaagc gattgccgca aaatcataac cctcttcttt gcgtatttta tctaatgtct
     4261 cttgaaatct acgatcttca attattgctt ctggtgtcaa atcctttcac ctcttatgct
     4321 tacactttat tcttacggta aataatatat ctgcgattta tatatgtcaa aggtacactc
     4381 caaacatgca ccaaacgtgt aaatggccaa caagccataa tagtgaaacc taacaatata
     4441 tgcattttaa atgcaatcgg cacaccactc atcaatgacg catctggttt taacataaat
     4501 aattgtctaa accaaattga taatgaagtt ctgtagttaa agtctggatg ttgtatattt
     4561 gttactaatg ttgcgtaaca tcccataaat acgataagta ataataagaa atttacaaat
     4621 atatccgacg ctgaacttaa tcttcgaata cttttcgtag taacacgtct cgctgttaat
     4681 aaaaacatcc ctatcaaagt tattatacca aagatgctac caatataaac agcgcctata
     4741 tgatataaat gctcagacac acccactgca tccatccatg gtttcggtat taacaatcca
     4801 actacgtgtc caaaaaacac tggaataata cctaagtgaa ataataaact tccccacatc
     4861 aacctttttc tttctattaa ttcactagat ttagctgtcc aagaaaattt atcataacga
     4921 taacgtgcaa tatgacctgc gacaaagaca actaaacata aatacggaaa tataacccat
     4981 aaaaactgat taagcatgat gtttcactcc ttttggtgat gtcaaacata atttcaatgt
     5041 ttttctaagt gcttgaatca cataggcata tggattgtta tcttcaccaa gtgcattcgc
     5101 catcacatat gttccatcct caataatcat aatgattaat tgaatattct cttcagctct
     5161 tggatcattt cgccattctg ccacttgcaa aaattgaagc atcaacggta gataatcaga
     5221 aagttcatta tctaccattt ctagtccaaa catttcatat aataccttta atttagctaa
     5281 catttgccca cgttcttttt gcgtatcaaa tttgttatac gtcatatata atggtgcttt
     5341 tttcgtaaaa tcaaatgtat ctgtataaat cgctttgatt tctgataatg aaaatgtcat
     5401 cattacattt ctaaatgcta ctaaatcttc atatgctgga tgatctttcg aaattgtttc
     5461 ttcaaaagtt tttggatgaa aagttaattt ttctggaaaa cataactgtt gtgccatata
     5521 tccaaaactt tcttgatatt ttttaaaatt atcgaaatta atcacggaaa atccctccat
     5581 agaaattctc attataaatt tcttgaccag ttttccctga acctactgca acgccacagc
     5641 cttcacagtt atctccaaaa tgctcgccgc cgtaattgta tcctgtacta ccttgtgcgt
     5701 gatacgtatc taaataggtt tctttgtgtg atgttggaat aacaaatcga tcttcatatt
     5761 tggctagtcc taataaacga tacatgtctt tagtttggcg ctcggttata cctaatcgct
     5821 ctaatcgaga cgtgtcaaat ggctgttgag taacttgaga tctcatataa cttctcatca
     5881 ttgccatacg ttgtagggct ccttttactg gctctgtatc tcctgcagtg aaaatattag
     5941 ctaagtattc aataggtaaa cgcatttctt caatggctgg gaaaatcgca tctggatttt
     6001 gagttgtatt tttaccttca aaatagctca taattgggct aagtggtggg caataccaaa
     6061 ccatcggcat cgttctaaat tcaggatgta acggaaatgc aagtttatat tcaattgcta
     6121 acttataaat tggagagttt tgtgcagctt caatccaatc gtaaccaata ccatcttttt
     6181 cagcttgagc aatgacttct tcgtcaaatg ggtttaagaa tatatctaat tgtttttcat
     6241 ataaatcttt ctcgtctact gctgaagctg cttcatgaac tcgatctgca tcatataata
     6301 aaacacctaa gtaacgcata cgtcctgtac aagtttcaga gcataccgta ggcatacccg
     6361 cctcgattct cgggaaacag aaagtacact tttcagcttt gttcgttttc caattgaagt
     6421 aaactttctt atatggacaa cctgtcatac agtaacgcca tccacgacat gcgtcttggt
     6481 caactaatac aatgccatct tcatcacgtt tatacatagc acctgaagga cacgatgcaa
     6541 cgcaacttgg attcaagcaa tgttcacata aacgtggtaa atacatcata aaagtttcgt
     6601 caaattggaa tttaatatct tcttctattt tttggatgtt aggatctttt ggacctgtaa
     6661 catgaccacc tgctaagtca tcttcccagt taggtcccca ttcaatttca atgttatccc
     6721 ccgtaatttc tgaatacgct ctagcaactg gcgaatgctt ccctgatttc gcagttgtta
     6781 aatgttcata attatagttc catggctcat aataatcttt aattaatggc atatctgggt
     6841 tataaaaaat tttacctaaa gcaatttttg aaattctact tccagatttt aattcaagtt
     6901 tccctttacg atttagtacc caaccacctt tgtagtgttc ttggtcttcc caacgtttcg
     6961 gataccctac acctggcttc gtttctacgt tgttgaacca catgtactca gcacctggac
     7021 gatttgtcca agtgttttta catgtcacac tacacgtatg gcatcctatg catttatcta
     7081 aatttaatac catcgcaact tgcgctttaa tcttcaagcc aattaacctc cttcatcttt
     7141 ctaactgcta catataaatc cctttggttc ccaattggtc cataataatt aaagtgataa
     7201 ctaatttgtg cgtatcctcc gactagttgt gttggtttca aatggattct agtcggcgcg
     7261 ttgtgtgaac caccacgtgt atctgtaatt tctgacccag gcgtttgaat atgtttatct
     7321 tgtgcatgat acataaacat tgtaccttta ggcatacgat gcgaaataac tgctcttgcc
     7381 gttacaacac cattacggtt atacacttct agccaatcat tatcttggat atcgtgtttt
     7441 tcagcatctt catttgatat ccaaaccgtt ggaccacctc taaatagtgt caacatatgc
     7501 ttattatctt gatacattga gtgtatattc cattttccat gaggcgttaa ataacgcagt
     7561 accaaagcat ctgtaccacc tttaattttc ttatctctat tcccaaatac cattggcggc
     7621 aatgtcggtt tatatactgg taagctctcc ccaaattgtt ggaaaacttc gtgatccaca
     7681 taataacttt gacgtcctgt taatgttcta aaaggtacta gacgttctat attcgttgta
     7741 aatggtgaat atcgtcgacc ttgtttattt gaacctggga atactgctgt cggtattact
     7801 tctcgtggtt gtgaagttat atttaaaaac gaaattttct cagcagcgcg ttcgctagaa
     7861 atatctttta acggcattcc agtttgttct tcgagatctt catatgattt ttgtgataat
     7921 ttaccattcg tagcagatga aatacttagt attgcatcag ctacattacg tgctgtatca
     7981 atacgtggac gattcgctct cacagaatca tcatttgtat cactccacgt acctaacata
     8041 ctttttaatt cttcatattg ttcactgaca ccgaaactta caccatgtgc tccaactttc
     8101 cctttttcaa gtacaggacc aagcgtgaca tatttgtcgt aaattttagt gtagtcgcgt
     8161 tctacaattg caaagttagg cattgtacgt ccaggtaccg cttcaatttc acccttcgac
     8221 caatctttca ctacgccgta tggtgttgaa atttcttgct ttgtatcatg actaagtgga
     8281 gttgtcacaa catctttaaa cgttccaggt aaatagtctt ttgccatttc tgaaaatgct
     8341 tttgccaacg ttttataaat atcccagtct gaacgcgatt cccataacgg atcaatggca
     8401 ggattgaaag gatgtacata tggatgcata tccgttgatg ataaatcatg tttttcatac
     8461 caagtcgctg ccggcaaaac aatgtcagaa tataacggtg ttgccgtcat tctgaagtct
     8521 aaagagacca ctaaatctaa cttacctgtt gtttcttcac gccacgtaat ttcttctggc
     8581 ttttcatctt catttggtgt agctaataac cctgattttg tgccaagtaa atgcttcata
     8641 aagtattctt gaccttttgc agaacttgaa attaagtttg aacgccatat aaataatgat
     8701 tttggatgat tctttttcaa atcaggatct tctattgcaa attgtgtttg ttttgatttc
     8761 acttcatcaa ttgcacgttg caaaatcgct tcatttgaat ctataccttc atctttagct
     8821 tcttctgcaa acaacaaact atttttatta aattgtggat atgatggtaa ccaaccaagt
     8881 ctagctgcta aaacattata atcagctgga tgttgatgct ttaactcctc tgttttagct
     8941 aatggagatt ttaaacgatc tacatttgac tcttcatatt tccattggtc tgttgcaaaa
     9001 tagaaccaac ttgttccgtt ttgcaaacgt ggtggtcctt gccagtcttt cgcaaatgcg
     9061 acagtactcc atccttcaat cggacgacat ttttcttgtc ccacatagtg agcccaaccg
     9121 ccaccgttca caccttgaca gccacataac ataactaagt ttaagattga acgataaatc
     9181 gtatctgagt taaaccaatg gttaataccc gcacccatga taatcattga acgcccttca
     9241 gtatcgatag cgttttgcgc aaattctttc gctacttgaa tgacaacact ttgttttacg
     9301 cctgaaatgg cttcttgcca agcaggtgta tattttgatt ctgcatcgtc gtatcctttt
     9361 gattctaatt tatgatcaaa acgacgcacg ccatattgac ttgccattaa gtcaaaaatt
     9421 gtagcaatac ggactttgtc accatttgct aaagtgactt gtcgagttgg aattggacga
     9481 ttgaatatcc catctccatc actatcaaag tatgggaatt gaattgtttc taattcgtat
     9541 ccaccttctg tcattgataa tgtagggtta attttagaac catcttctgt ttctagtttt
     9601 aagttccact tcttaccttc ttcccaacgt tgacccattg tgccattagg tactactaaa
     9661 ctatcgctga ttgcatcatg aataactggc ttccattcgc cttgctctgt tgtttgacct
     9721 aagtcactcg ctcttaaaaa tcgacccgct ttatatccat tttcatcttc atccagcatg
     9781 ataagaaacg gcatatctgt atattgttta gcgtaattta taaagcgttc attaggttga
     9841 ttaacataat gttcttgtaa aataacatgc gtcattgctt gtgcaattgc agcatctgaa
     9901 ccaggattcg gtgctagcca gttatctgca aatttcacat tttctgcgta atctggtgct
     9961 actgaaatga cttttgtacc tttatagcgg acttcagtca taaaatgtgc atccggagta
    10021 cgtgttaaag gtacattaga gccccacata ataatgtatg atgcgttata ccagtcactt
    10081 gattcaggca catctgtttg ctctccccaa atttgtggag aggcaggtgg taaatctgca
    10141 taccagtcat aaaaactaag catttcacca ccaagcaaat tgatgaatcg agcacctgct
    10201 gcataactaa tcattgacat cgctggaata ggtgtaaatc ctgcgattcg atctggacca
    10261 tattttttta ttgtatacag taattgtgct gcgattatct ctgtaacgtc tttccaattt
    10321 gaacgcacgt gccctcccat acctcgggct tgcttatatt gtttggcttt gtcttcattt
    10381 tcaacaatag acgcccatgc agcaacgcga ttaccattgt tttcttctaa tgcttcagtc
    10441 cataaatccc agagttttcc acgaatatat ggatatttga ttcgaagcgg actgtattca
    10501 taccaagaga atgacgcacc tcgtggacat cctctcggtt catattcagg catatccgga
    10561 ccacaacttg gatagtcagt ttgttgattt tcccaggtaa tcacaccatt tttcacaaat
    10621 actttccaag aacatgagcc tgtacagtta acaccatgtg ttgttcttac ttctttatcg
    10681 tggctccaac gttctctgta cattttttcc cattctctac ttttactttc taggatcgac
    10741 caattcccat taaatttttc tgttggctta aagaaattca atccaaattt tcccatattt
    10801 atatcctcct acgtataaaa atacgatgtg tagatgtcgt gtttttaaat actttaaaat
    10861 gcccaagact attgctttaa ttagattgta cattttttca caaacataaa atattaggga
    10921 atcacctaat tacttaagga atttccctat caataacggg atttcattga aataatacac
    10981 aatcatgtat ggtcatgctt attgccaatc taaatcgttc aaatttggca caacgacaaa
    11041 taaggcttca acacgaatat attctctcgg ttgaaacctt acttattcat ttatttttta
    11101 taaattagtg acataacact gtattagcat ctgcacgatc ggttgaaata tatgttacat
    11161 tttcttgctg cttaataaat gcatcatagt aatcatattg cgacgaatga tatgtgccat
    11221 tcgatgtatc atttgggttt agcaaacagc cataaccttc gtcatataaa tgttcacaga
    11281 gcataagggc gtcatgttta gaaccactta ctacataaaa ttgcttcata ggatcatatg
    11341 atttaggagt gttttcagta taatcaacaa cttcccctat aatacatata cctggtttcg
    11401 cctcaattga atagtgttgc aattttgaaa taatattact taaacgcccc ttaacaacaa
    11461 actcgttaaa acacgatgct tgaaagacaa tcgctatcgg gtaatcaata tctgtgtatt
    11521 gttgtatctg tgtgataatt ttccctaaac gttttacccc catataaatt gctaacgtgc
    11581 caccattcac taaggaattg acatccactt cattttcttc tgaatcttta aagtgacctg
    11641 tagaaaatgt cacactttta gcaactgtac gcattgtcaa acctgtctgc atagtagcaa
    11701 ctgctgcgct cgctgatgtc acccctggta caatttcaaa cgcaatatga tgttcattta
    11761 gtgtgtcgac ttcttcttgc acacgaccaa atatcgctgg atcgccacct ttaagtctaa
    11821 caaccttgtt atatcgacgc gctgcttcca cgatacagtc atttattttt tcttgctgaa
    11881 tatgttttgc atacggcttt ttaccaacat cgataatttc agtagttaaa ttcgcatatt
    11941 gtaaaattaa cggattcact aatcgatcat atagaatgac atccgcttca cgtattaaac
    12001 gctcagcctt tttcgtcaaa taattcggat tacctggacc cgcacctatc aagtaaacct
    12061 tgccatattc ctctacagac atatatatac gttcccgtct gtaacttcta cctcataaac
    12121 atctacacaa ccttcatcag gttcttgaac aatacctgta tttaaatcaa ttttttgatc
    12181 gtggagcggg caaaatacat attccccact cactgtccct tcagacaatg gtccttgttt
    12241 gtgtggacag atattgtgaa tcgcatgaat tttgccactt tctgttaaaa acaaccctac
    12301 ctctttgcct ttgacaataa ccttttttcc aattaggggt gttaattcat ctatagttgt
    12361 cactttaatt ttttcttttg tttccatgta ttacaccttc tccacttcaa aaattctacg
    12421 tgcttgcgca ttgctagtta ttgcttccca aggttcagct tcgactgctt ttttagcatc
    12481 cataatgcgt tcaaatagtt cattttgtct ttctgggtca agtaagactt cttttacatt
    12541 ttcaaatcca agtcttctta accatggcgc tgttctttca gcatatatac ctgtttcgcg
    12601 ataatattgc atcaaagcac cacataattt gattacttca tcttctgttt ctacagttgt
    12661 taaaaattca gctttttcaa cttctgtacc accattacca ccgatataga tttggaatcc
    12721 attttcaact gagataatac caaaatcttt aacacctgat tcaacacaac ttcttgggca
    12781 gcctgataca cccattttga atttatgagg tgtatcgatg tattcaaatg ttttttctaa
    12841 acgaatgcca agtcgtgtcg tgtattgcgt accaaatcga caaaactctt taccaacaca
    12901 gcttttaact gagcgtgttt tcttaccata agctgatgct gaacgcatac ctaggtcttc
    12961 ccatatattt ggtaattctt cttttttaac tccatacaaa ccaacacgtt gtgaacctgt
    13021 cactttaact agtggcacat gatatttctt agccacttct cctagacgaa tcagttggtc
    13081 tgcatctgta acacccccac gcatttgagg tataacagaa aatgtaccat cattttgaat
    13141 attcgcatgg taacgttcgt tagcaaatct tgattctctt tcatcttcat gatcatgtgg
    13201 ataaaccatg tttaaataat agttgattgc tggtcgacat tttggacatc cacctttatt
    13261 tttaaagttt aaaacatgtc gaacttcttt agatgttttt aaacctttcg ctcttatttg
    13321 cgttactatt tgatcgcgtg tcaaatcagt acaaccacat ataccagcag gttttgcggc
    13381 aacaaagtca tctcctaagg tgtgctgcaa tatttgagca atttgcggtt tacatttacc
    13441 acatgaattc cccgcttttg ttttagccgt tacttcttca actgttgtaa agccattttc
    13501 cgtaatcgca tttactatag tacctttatc aacaccatta caaccacaaa ttgtttcatc
    13561 atcagccata tcagcaattg atagcgatgc ctcttctcca cctttagtaa gcaatgatac
    13621 aagtgtgtaa tcttcagtgg attcaccttt tttcatcatg ttataaaagc gtgaaccatc
    13681 atcgatatca ccatatagta ctgcaccaac tacattaccg tcttttaaaa agattttttt
    13741 atagttatta tcaacactat taaatatttc aataccttta atttctgcat tttctacaat
    13801 ttgaccagca ctatacaagt cacacccaga aacttttaat gacgtaaatg ttgttgatcc
    13861 cttgtatccg ttcgtttctt tatttgttaa atgatcagct aatactttac cttgttcata
    13921 tagtggtgca acgagtccat aaactttgcc gttatgttct gcacattcac caactgcata
    13981 tacattgcta tcacttgttt gcatcacatc attgacaaca ataccacgat taacatctag
    14041 acctgattct ttggctactt ctgtgtatgg tcgtatacct actgccataa caactaagtc
    14101 tgccggaatc tcgcgtccat cagccaattt aacaccctca acatcatctt ctcctaagat
    14161 ttcagttgtg ttggcttgca tttcaaactt cataccttgc ttttctagat ctgctttaag
    14221 catatttcca gctttacggt ctagttgcat ttccatcaac cattcagcta aatgtaacac
    14281 cgttacttcc ataccttgat ctaataaacc acgtgcacac tctaaaccta gtaatcctcc
    14341 accaattaca attgctttct ttttagtctt agcaatgttc atcatttgtt cagtatcatc
    14401 aagcgttctc caaccaatta cactcggtaa tgtcgaacct ggaactggaa tgacgaatgc
    14461 tttagaacct gtcgcaaaaa tcaatttatc gtatgatact tcaataccat ttgcagtagt
    14521 aactgattga tttgctctat ctacttcaat tacaggatca tttgtaatta actcgatacc
    14581 atgttcctca taccactcat atggattcat aattgtttct tcaactgtca ttttattttg
    14641 taaaatattt gaaagcatga tgcggttata gtttggataa ggttctttac ctattaccgt
    14701 aatatcatat aaatcgttgg cgcgctctaa tatttcttcg attgttcgaa tgcccgccat
    14761 accgttacca atcattacta gtttttgctt tgccataaaa tatgcccctt tactccataa
    14821 tatttatttc aaaaaaaggt attaattttt cgttagtgct tttatatttt cattggaatc
    14881 attaagcttt ctaatctatc gttaatgatt tgctttaaaa ttgggtcgaa gttaattgaa
    14941 ggtgtgaagt gtatatctgt attaataacc atgtcattca tttgctgctt cactttgtta
    15001 acaagtcttc cgtcatataa aaataatggt acgacaatca atttttgata ccgtttcgag
    15061 atgctttcta aatcatgtgt aaaactaatc tctccatata gcgttctcgc atatgtcggc
    15121 ttgctaattt gcaaattttg agcgcatatt tgtaactctt cgtgtgcctt agtaaacttt
    15181 ccattaatat tgccgtgtgc aacaaccata actccaactt gttgttcgtc acctgctaat
    15241 gcgtcacaaa tacgttgttc aattaatcgt ctcattaaag gatgtgtgcc aagtggctcg
    15301 cttacttcta cctttatgtc tggataccgt cgtttcattt catgaacgat attcggtata
    15361 tccttgagat aatgcattgc actaaagatt agcaatggta caattttaaa atggtcaacc
    15421 ccactttgaa tcaacgtcgt cattaccgtc tctaaatcct gatgctcact ttctaaaaac
    15481 gcaatatcat agtgatgtat atcatctttt actaattcag aaataaatgc ttctaacgct
    15541 tgattctgtc gtccgtgcct catgccatgt gcaacaatga tattcccatt cacatttacc
    15601 aaccctttca cacgtattgt ataccaaatc attttgtttt tgtgaaaaga atcacattat
    15661 aatgtaaaat cagggaattc cctgatgcct gtagtcatgc atattcctta tacattttcc
    15721 ctttttgtta aatcaaaaaa agcgaccgat atatgaatcc ctactcaaca tttatttgag
    15781 caagcattaa tatatcggtc gcttgtagtg tatattatta tcttaaaatg gtggttggcc
    15841 taatattgtt tcgtcaaagc gctcgggtat caatactttg cgcatgatca cacctaaatc
    15901 gccatcatca ttttcatgtt cgctgtatat ttcataacct cttttttcat aaattttaag
    15961 taaccacgga tgcaatcttg cagatgtacc taaagtaact gccgctgact ttaacgtatc
    16021 tcgcaaaaat gcttcttcaa cataagtaag taattggcta ccatagcctt tcccttcata
    16081 ctcaggattt gtcgcaaacc accagacaaa aggataaccc gaaatacttt tcacacttcc
    16141 ccaaggatat ctaaccgtaa tcgtagatat aatttcatca tcaattgtca tgacaaatgt
    16201 agtattttta tctatatttt ctttaacagc atctaaatta gcattaaccg aaggccaatc
    16261 aatacctagt tctcttagag gcgtaaatgc ttcatgcatg agttgttgca atttttctgc
    16321 atcttgttca cttgcgagtc gaatcatcgt ttttgtcata ttaatcccca ctctttttta
    16381 aatgatttaa ccatatttta tttttaaaat aaatatccat caaagtgtat caataaattt
    16441 atcacatgtc agaaagtatg cttcatctga atacaccaat actctcatga aacttattaa
    16501 aaattactct ctcaacgtaa aaaaccattc aaattcatga atggtttgga agaatgattc
    16561 attgttacgc tatttaatca ctacatctta attattgttg ctctaaacga ttacgcttac
    16621 catttaagaa agcataaacg agacctacaa aaataccgcc accgacaaag ttacctaaga
    16681 aagcaaaaac gatatttttt aaaacgtgta accatgaaac tgcatcaagg ttaaagaata
    16741 ccatacctgc atatagacct gcattgaaca caacgtgctc atatcccatg tatacaaaga
    16801 ccacgacacc acaagctatg aagaatgcct ttgttaagcc gcctttgaat tgcatagaga
    16861 tgaaaatacc aatattaata aagaagttac agaaaatacc ttttgtaaaa atattcaacc
    16921 atgttgaatc aacagtcttt ttctgaacta aagctgttaa agcttgtgtc atttcaggtg
    16981 tcatgacatg cgcaaatttc attaagaaaa ataacacaaa cccacctaaa atatttccta
    17041 agaaacaata taataaaatc caagtcatct tcttaacaga aacgacttta taataccagc
    17101 ctactgtaaa gtacatgaag ttacttgtta ataattcaga gttagttaat acaactaaaa
    17161 tcaaacctaa actaaacgca atggctccca ttaaattgat aagtccatct acgtgagtac
    17221 tcgcaaactg cgtctttatt ccgaacataa atacagtaac gattgagagt aaaaatccgg
    17281 ccatcattgc tttaagcata tatcggccag gtgtttgctg taccattact tgcttcattt
    17341 gtactgaaga tgatattgaa tcaacaattg cgcctgttga atatgtatct tcaactgttt
    17401 tcttattttc tatcacagta cttcccccat ttactttgat caaataaaat ttactttcta
    17461 attatattag taaatcaatg taattgatag attaaatttc agaaaaatct ttttattctc
    17521 tcgaaaatgt agttttcttt cataaaaaca gcgagataaa tcgcgtgatt ggctaatatc
    17581 tttatgattt aagatattac gacgctcatt ttatctcgct ttatgtaaag tattacttat
    17641 tcttttttaa atttgaaaag taatgtaaac catatgcaaa tacactgtaa ccaattgcag
    17701 tacgtaaaat aaaccttata actttattag gtacacattt tgacgttact gaaatagaag
    17761 atagtagtgt tgtgaaaata tataatatta aaactttgat ataacgttct accatcgaat
    17821 catccctcat ttttataata ttacaagatt attataaacc gaatgaaaca aaaaagtttt
    17881 tacatatttt caacaacttt atttcaataa agcatcaagt tgatctaaag tttgattcat
    17941 accttgttca acacccatgt ttatgacttg ttgagcggct tcttttgttg gaaataccga
    18001 tgttgatgtc actgtcgttt tcgttgtatt gctcttggaa aatgacaaag taattttcat
    18061 gcttggcatt tttgtatctt ttgcaccttg aggtgtcgca aatgagtcaa tatattcaat
    18121 taaatatgga cgcttaaccg ttttatattc tgctaatgta tagctggtca ttgtaggtgt
    18181 tttaatcgca taaaatgcat caccgcctga aacagcatta aagcgaaaca ctttggtact
    18241 agcgtctttt ggatgaaacc atttttcaaa taatgctttt gtcgtatagg catcaaacac
    18301 cttttcaatc ggtgcttcta tcgttcttga aaaaataatt ttattatctt caactttaat
    18361 cgtcatcttc ccactcccta tcttttatat ttactcatct taacatgcat tgagtaacat
    18421 atgttaccaa atcatttgtt ataaactata gtaatattaa tcattccctc tgttgaaatt
    18481 gataactacg aaataaaaaa cacactctat tcagctatta acagttgagt agagtgtgca
    18541 aacctgttga attaatgcgc taacatttct tctttgattt ggtctttatt taattttgaa
    18601 ggataatatg ttggccagtt atccatttct tttaataacg cgtcattatc attacccata
    18661 aagatatgga agtgttctgc ctttttaggt gcaatgttgt gatcgctaaa ttggatgaat
    18721 ttcggtaagt ctttatcatt accatcgacc aatttaaaca taaatctcac gccacggtta
    18781 cctttaggat atttcaatgt tttcttacca ttgtattcat atttaccagt gtgtttctta
    18841 ccatctttag taaatgtaat ttcatttcct tttatatcaa tgttagtaat atcagtttta
    18901 tatcctttgt cataataagc ttttaaatct ttagcagatt tcttcggatc attttcagct
    18961 ttatgttcca tcacttcatc aagcgtaccg tcttttaagt aagggtaaac agattgccat
    19021 tcaccagcat aatcgcttaa ttcacggtct ttaacttgct catctttaaa atacccatca
    19081 gaaattgctt tgtcatgttt actttcagct ttgtcgtctt tcactttaac accactgtct
    19141 aaagctttac cgatattttc aatattagat ttcattaatg attgatacgt aatattatct
    19201 tttttctgtt gttctttatt taaagactcc atgttgtaga attttaaagg cttcgcatct
    19261 gtttctttac gaattgtttc agtcacttta ttcgcaacat tatcttcgta aagaatatat
    19321 ttagcattgc tatctctaat ttctttaaca attttagtta attctttttg tgatggatct
    19381 tcagcattca tgttttgaat acctttttga acaaaaccat aacaatcagc taagtatcca
    19441 attgattcat gtgaaatgaa tactgcatta ccttgcttat cttttgtaac ttgcttcata
    19501 tcgttatcaa ttttcttaag atcgtcgttt aattttttgt agtttttctc atagtcatct
    19561 ttatgttttg gatctttttt cactaattca tctttaattt ctttagcgaa agtttggtta
    19621 attttaggat ctaaccatac gtgtggatca tatccaccat gatgatgatg atgttcttct
    19681 ttctcatgat catgtccctc atgttcatgc tcttcaccat gctcgtgttg atcagttaaa
    19741 agctttgctt tatctaattt atcctctaaa gacagttttt tatctttatc tttgatagta
    19801 gatgcaactt tcttagcaac cggatctaaa ttatcccctg tatacataaa caagtctgac
    19861 ttgcttgcac ttaatatatc tttttgtgtt ggttcatagc tatgtaaatc tgtccctgct
    19921 ggatagattg atgatacctt cacgtgtttt ccaccaattt gctctgcaaa tgattgcaat
    19981 ggataaactg tcgttttaat tgttaccttg ccatctttat cttttccatc atcattccca
    20041 catgcggcta atgataaagt tacggctggt acaagaagta acatacctaa tttctttttc
    20101 atagtcaccc tcctaaatag taatctttac gttttaaaat tatatactca tgctacttaa
    20161 aatacaagca ctgtttttat aaataatata aagtcgaatc atactttagg tatttaccac
    20221 ttttcgtttt atctaataca tgactattgt acaaatatga tacagaaaac tttatagtat
    20281 ttacgaacgt cataatataa gaaaaaaaga caatctctat catattgcta atagaaattg
    20341 ccttttcata ttgtcctaaa ataacttacc ctatttttat agtgtgactt ttagatttaa
    20401 taatggtatt tacatgatgc tatttttata tgattgtcat ctactgtata aaccaatcta
    20461 tgttcgtgat tgatacgtct actataatac ccagtcagat tcgattttaa cttttcaggc
    20521 ttacctatac cttccaatgc accatttctg tcaatacttt taagtaactc attaatcttc
    20581 ttcaccattt ttttatcgtt ctgctgaaaa tacttataat cttcaaaggc ttgaggcgaa
    20641 aacgtaatat ttaacctagc cattatacat ctatatcttt cgttatagtt ttcccacgtt
    20701 ctagatctgc aattgattga gctaagtgtt cagcattatt tgggttctgt tggaggtaaa
    20761 gtgtttccat catggagtta taatctgatt ctgacatgat tactacgttt ttatcatcag
    20821 tagatgttac agttaccata acactatcat catttacttt tgtcataagt gcctttaaat
    20881 tctgtcgagc gtatgaataa tttttaataa tcatatttat cgcctccttt taacacacct
    20941 caattgtaca aatatctgta caataaaaca agcgacacag aaaaaaacta ctaatcaaga
    21001 tttcgcacgg ggaagaatca atacctatcc tgtactgatt ccctactaaa caatgtttac
    21061 atgataattt ccacctattt agacttcata cattaaaaag acaatctcta tcatatcgct
    21121 aatagaaatt gccttttcgt attgtcctaa taaattggta tcacttactg atctcaacaa
    21181 ttaagactat ttgattttat cttttaataa cttctcataa ctttcataat catatggatc
    21241 ttcaactttc tcgccattaa taaatgcagt tggtgtcgtt tttatatgat tatctttcgc
    21301 tattttttta tctttctctg cagctttcca agacttgcta tcttttgtct tgtaatcttt
    21361 tataatttta ttttccgtct ctttatctaa atgcagttgt ttaatatgtt tatctaatag
    21421 ttcttttgtt aaccattctt tattttcatc ttgctgggca gcaaataatt gcttttgaaa
    21481 atctaaaaat gatttaggtg catacatcaa tactgcatga ctcgcacgcg aaccaacaat
    21541 tgagtcttta cctaagaaag ctaaattgac aaattggtat tccactttgt gattatctat
    21601 ataattttta cgcaactttg gcatgacttt ttcatctaat tctttacaat aaggacattt
    21661 atagtcgcca tatacgacaa ctaatggttt gccgtttttc gaagatgtcg ttgctgattc
    21721 ttttttaccg caagctgtta aaattaacat gctcactata aataatgtta gtaatttttt
    21781 agtcataata actcctattc atatctagtt cgtcatattt tcttcatgat aatccgcatc
    21841 atgttgttga taatatcgtc tcgaatcaaa atcttgctta tatgatacac gtttatcact
    21901 aaaatcatat gcataataat gcacttcatc attactcaaa ggtttataag ctaaaatgat
    21961 aattttatcc ttatcataga cataaatatt agcatctttg cgttcaaaat gatcaacacg
    22021 atcaccgttg ccttttttgg caagttgttc ttgtttttga ttttgaattt taactgcttc
    22081 atctatttct ttcacatatt tatcattacc acatcctgat ataaagacta gtaataataa
    22141 taccgtcgcc acaaatcgct tcaatgctcc cactcctact atcaacttcc aactaacttt
    22201 caatataatt aatactatac accgtcaaac attaatgcgc tacaactact accaatgcat
    22261 ttaatataaa tatccaaaga aataacagtt atgtatagtc aaagaaaaat atatttcatt
    22321 ttctatgtgt atttgtaatc agtttttgat aaaataaaaa aatagcatta ttaataaatt
    22381 caaaaattat agttcaattc agagagggat gggataaaat gaactttgta acgttgactt
    22441 cggatgagtt caatgcgttt acaacaaagc atttttcaca ttacacacaa tcagctattc
    22501 attacaatca tagagttgat ttaaaaggcg atgtgcatct tgtaggggtt aaagatgaca
    22561 atggtcaagt gattgcagga tgcttattga cagaagcacg cacacttaaa tttttcaaat
    22621 atttttatac acatcgcggg ccagtgatgg attatacaaa tcaatcatta gtagcatttt
    22681 tctttaaagc attaacgtca tatttaaaga aacacaattg tttatatgtc cttgtagatc
    22741 catatttaat tgaaaattta cgcaatgcag acggtgaaat tgttaaatct tatgataacc
    22801 gagcatttgt tagaacaatg gataaattag gttataaaca ccaaggtttc cctgtaggtt
    22861 atgattcaat gagccaaatc cgttggctgt cagtgttaga tttaaaagat aagactgaag
    22921 accaactttt aaaagaaatg gattatcaaa cgagacgtaa tattaaaaaa acatatgata
    22981 ttggtgtcaa aactaaaacg ttaacgattg atgaaacgca aacttttttc gacttattcc
    23041 atatggctga ggaaaagcac ggtttcaaat tccgtgagtt accatacttt gaagaaatgc
    23101 aaaagttata cgatgaccac gccatgttaa agttggcgta tattgattta aacgagtatt
    23161 taaaaacgtt acaattaaag caacaacaat taacagctga actctcaggt gttgaagaag
    23221 cattggaaga aagtcctaat tcaaagaaaa ataaaacgaa acgcacacag ctagaacaac
    23281 agctaaatag caataagcgt aaaatcgaca atacaataga acaaattgaa caagatggtg
    23341 cagtcttaaa cttagcttct gctttattta tctacaatga gcatgaagtt tattacttat
    23401 ctagtggttc aaaccctaaa tataatgctt atatgggtgc ctaccatttg cagtgggaaa
    23461 tgattaagtt tgctaaagcg catcatattg atcgctataa tttttatggt attaccggtg
    23521 acttttcaga gtcatcagaa gactatggcg tccaacaatt caaaaaaggt tttaacgcac
    23581 atgtagaaga gtatatcggt gactttatta agcctatcaa actattacta tataaggtac
    23641 aaacgtactt aaatcataaa cgtcgttaaa atatcgctcc aagattatga caacataaca
    23701 aagaaccacg catcatgtat atggcttcat cgccatacta aaacatgata cgtggttcta
    23761 tttgattatt cttcatttat aacatttaag aaacgtctta attcttctgt tttcggacga
    23821 ttgaacatct cttctggagc cccttgttct ccgatcatgc cttcatgaat aaatacaatg
    23881 ttattagata cttctttagc aaaacgcatt tcatgtgtca caatgaccat tgtcatgcct
    23941 tcattagcca aatctttaat aacctttaaa acatcattca caagttcagg atcaagtgca
    24001 gatgttggtt catcaaacaa catcacttta gggttcattg ctagtgctct tgcaatagca
    24061 acacgttgtt gttgaccacc tgataatgca tgtggacgtt gatctttgac atgtgttaaa
    24121 ccaactttct caagtaactc aagtgatttc ccacgtgcct catccttttt caacttttta
    24181 actgtgataa gaccttccat tacattttct aatgccgtct tatgcggaaa aaggttataa
    24241 ctttgaaata ccataccaga ctgtttacga acttctattt gtgatttttt atctttagat
    24301 gtatatgttt tgccgttaac ataaactgta ccttcagttg gaatttctaa tgcattaatc
    24361 atacgtaaca atgttgtttt acctgaacca gatcgaccga ttaaggttac aacctcacct
    24421 tgttcaacag ataaatcaat acctttgatg acttcaacat cattaaatga tttatggata
    24481 ttgttcaatt gaatcatgag cgataccctc tttcaatata agattcatag ataccttgga
    24541 taattgaaat aataaagcat accacccagt acattagcgc aactaacaca taaattgtta
    24601 aatattcata tgttgtagac gcaacttctt gagcttttct aaacatttca gccactaaaa
    24661 taaatcctaa taatgatgta tctttgatta aacttaaaaa tgtattacct agtgcaggaa
    24721 tcgacactcg aattgcctgc ggtaaaatga tacgttgtat cgtttgacca tacgtcatac
    24781 cgatggaata tgcagcttct gtttgtcctt tcggtataga aataataccg ccacgaataa
    24841 tttccgaagc atacgcacct acatttaatg ataaaccaat aatagcagcc actacaggtg
    24901 ccaatgtcca ttggttgtca gcgtcatttg ttaataatct acctaattct ggaataccat
    24961 aaaatatgat aaatagttgt actatcattg gtgttcctcg aataatagat acatagacac
    25021 gtgaaatact tcttaaaatt ttactcgttg aaattcgcat taatgctgta aataatgcaa
    25081 taatcaatcc taaaacaaat gtaactaatg taataggaat tgaatatttg accaaacctt
    25141 ctagcatagg tccgaaagct tgttttgcag catctagcgc atgttgttgt tcgctattta
    25201 gatttagaaa catcttgacc aaaccatttc ttacctattt tagctaattc accgttttcc
    25261 tcgatttttt tcaagccatc attgaatttt tgaactgttt catcatctgc ttttttagaa
    25321 aatgcaaatg cagatctact ttgttcagca ttacctttga ttgctttgat cttagcatta
    25381 ggtttttgtt ttttataatc caagtatgac agactatcat taaatgtacc atcaacacgc
    25441 ttagacaata ataaatccat tgattggtta aagccatcaa cttttgtaat atcagcacct
    25501 ttatccttag ctaatttacc ataattagat gtgaatgttt gtgctaactt cttaccttta
    25561 acatcatcaa aatctttaat atctttttca ttttcacgaa taactaaaac agcacttgag
    25621 aatgtgtaag gcttagaaaa tttatatttc ttttctctat caggattaat acctacttgg
    25681 ttcgcgatta catcaaaacg ccctgcgtct aaacctgcaa acatagaatc ccaagaagtt
    25741 tcattaaatt taagttttaa accttcttct ttagccactg ctttaataac atcaatatcg
    25801 taaccagtta atttaccatc tttatcgtgg aaactaaatg gtgcataagt cccctcagtc
    25861 ccaacattaa ttgtcttgct atctttacta gcttccttgt ctttcgacga attgtttccg
    25921 catgcagcca atatgaaaac gaaagctatc atcacaaata aaagtctttt catagaatat
    25981 atctccttat tcttattatt ctaatcggaa taaaaatatt ttaatctgaa atgccggata
    26041 agtcaatatg ttgaactttt gctaaaaagc tttatatagc tagcaactta tatttattga
    26101 cgagaatcaa cttctttttt cttaccttgt aacataaatg aacaaaagaa actaacaact
    26161 gtaatcaata atgcaaataa aaatccagcg tgataaccat gcaacatcgt atcaacttta
    26221 atttgattca tcatatcaat tttgttcatg ccattatagt ctgacatagt tggagaaaag
    26281 tttttgcttg catgggataa aatagtaatt aatgcagctg ttccgataga accagcaatt
    26341 tgttgtactg tgtttgtcat agatgaacca tgtgcattca tttctggctt taattgattc
    26401 atcgtatggg tcattactgg aattaatcct aacgcgatac caaccattcg aatcgcataa
    26461 actgttgcca acatgattgt tgatgtttgt tcatccatta caacaaaata agccgtagta
    26521 ataacaacaa tcgccatacc tactaaagca agatttctag gaccaaactt ttcatataaa
    26581 gcacccgaag tcatcgacat aaatgccata acaattgctc ctggtaataa agttaatcca
    26641 gaatctaaag ctgaacgatg caataaattt tgcacaaaga taggtaaaat cgtttccgaa
    26701 ccaatcatcg ataccattgt gacacccata ataatgatac caactgtaaa ttgtttattt
    26761 ttaaatacag caaaatttaa taatggtgtt gatagccgat attgacgtaa tacaaataat
    26821 gcgactaaaa caacgcctcc aatcatagat actaatacga taggattatc aaaacctttt
    26881 tctgaaattg aactcgttcc atacaataac ccgccgaaac ctagtacaga aaaaatgaca
    26941 gaaataaaat caataggtac tttaattgta gttccaacat tttttatcgt tttaaatcca
    27001 attaaaaatg tcacagctgc aattggagcg acaacatgga aaagcgatct ccagttaaaa
    27061 tattcaacaa ggtaacctga aagagtaggt ccaatagctg gtgctaaacc aattaccaaa
    27121 ccaaatatcc ccatatattt accgcgttca tgtggttgga aaacatccaa aatcgttgtc
    27181 atcatcaatg gcatcataat acctgcgcct aacgcctgaa taattctagc aactaataat
    27241 gttgcaaagt gcggactcaa gccgccgagt aatgtaccta agaaaaagat agaaataccc
    27301 actaaaaaca cttgtcttgt tgtataacgt tgtataacaa gcgcggacaa aggaataaca
    27361 atcccattag tcaataaaaa agctgttgtc agccattgaa cttgtgtata tgaaatttca
    27421 aaatctttca taatacttgg taaagccgtc actaatagtg tttcatttaa taatccaaaa
    27481 aatccaccca tcagcatagt aataatcatc gttaattttt gtttcttcga catttaaagc
    27541 ttccccctca ccatctaaaa aacgacacac atttttagat atccggtaat tatacccatt
    27601 ttccaaaaaa ttagcacgac tttattacaa tttctttaaa acgtaatcat tactatttac
    27661 atatgagtag ttatcggtca taatatattt acaaggaggg atagagatga aattagattt
    27721 acagactgct cgtcgtaatt taaatagccc taacattaaa acaagaaagc gtgctttaaa
    27781 gattattaag caacataaaa gagcgaaata attgttttaa tttgactata ttgtatgtct
    27841 aactatattt tcgagtatcc ctagctcgtt aatataaact ctatacttaa ctatttcctt
    27901 acattacttc acatatttaa gagctgcata tacactcact ccttgtgtac atgcagctct
    27961 ttttttataa gtagtattta tctataactt ctaaatcatc cgttaattca taaacaagcg
    28021 gtgcacctgt tttaatttca taattaatga tatcttcatc tgacacatct tcaagatatt
    28081 taatcaatgc gcgaattgaa tttccgtgtg cagaaactaa taccgtttga ccatctagca
    28141 aatattgtga aatatgatct gtccaaaatg gtatcactcg aactaaagta tctttcagac
    28201 tttcagaata aggcatcata cgtttatcta aatgattata tcgacgatca gctaagtaag
    28261 cttcacgttg ttcttcggtt tcagcaggtg gtttcacatc ataagaacga cgccaaatat
    28321 gtacttgttc ttctccaaat tcttttctag catcatcttt atttaagcct tgcaatccac
    28381 catagtggcg ttcatttaaa cgccagcttt tatatacagg aatccattgt tgtttagatt
    28441 cagttaaaat ataatgcgtt gtatctaaag cacgtgttaa taacgatgta aaagctacat
    28501 cgatggcaat gttattttct cttacttttt cacctgctct agtcgcttca ttaatacctt
    28561 gttcagataa attaacatct tcccatccag taaataagtt tttagcattc cactcgcttt
    28621 gtccatgacg acataaaatt aattttggca taacttataa cctctttcct ttaatcttta
    28681 ggacgttatt tcagggatat tttaagatga tttaatctta gtaattttct tttcaatata
    28741 ttttaaaatt caatgattaa atcaattatc actagacatt aaatacataa atcctattcc
    28801 ccattttcat tttttaattc ataaatgaat caataaccac ctaataacaa atcatattat
    28861 acacctttgt tcgctatttt tctaaggttt aaaaaatatt tttaggtaaa cctaaaaata
    28921 gatgtaataa aaacgcctcc tcagatattt atatatctat gaagacgttt aaatacatta
    28981 tagatggtct ggttctgggt gaacgtatac tgaggaaata ccttttttgt gcaaatgatg
    29041 ttcgacattg tcacaaattt gatgcgcttc tactaaggaa aggttagcat ctacaacaat
    29101 tgtgacatca ataaacacac tacttccatg gtaacgccct ttaatacttt taacttcttg
    29161 tacttcatca acttctaaaa tatcattgcg atacgcttct aattcagttt cattgaaacc
    29221 atcactcaac ataaaaattg cttctttaaa aataccaaaa ccagtataaa cgattagtaa
    29281 gcctagtaat gttgctaaaa taatatcgac aattgggaaa ccgatttgcg taaaaattaa
    29341 tcctatcgct gttccaatgc tgactaaact atccgataaa ttatctttgg cagccgaatt
    29401 taaagaacta cttttcgttc ttttcgctag tctttgattg actgcaaata caatcaacat
    29461 tacaagacca ctgattaagc tgacgataat tgttattgcg ttaggtacaa cgtcatcttc
    29521 tttgaacaaa cgaggtgcat tttgaataac tacttggata cctacaaaca taatgacaaa
    29581 tgacaccaat aatgaagaaa tattttcaga cttcaaatgg ccataaggat gatttcgatc
    29641 ggcaggttta attgaaattt tcaatccaat aataacagct aaagaaacga taatatctgt
    29701 catattgttt aatgcatcgg ctcttacagc tgcagagtta aagacaaaac ccgtgacata
    29761 cttaacaata gataagatta tatatacaat taaactcaaa taagcaccgc gttgcgccaa
    29821 tttaagattt tcattatgag acatgcgttg aaccaccttg aattagtata gtaacaatat
    29881 tatgaatgat tcattttaat tttacaacgt ttttaat
//
LOCUS       DALAGD010000025        29811 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.025, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000025 DALAGD010000000
VERSION     DALAGD010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 29811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29811
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.025"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(102..1166)
                     /locus_tag="O2701_000887"
     CDS             complement(102..1166)
                     /locus_tag="O2701_000887"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCZ4292792.1"
                     /translation="MYKLIKPFLFKIEPEKAHGLTIDALKTLQKFPVLFPVVDKLFTY
                     KNPTLSQTIQGNTYDNPIGLAAGFDKSCEVPKALEHLGFGALELGGITPKPQPGNPQP
                     RMFRLLEDDALINRMGFNNIGMNKALSHLRKNAYQVPVGINVGVNKMTPYEARYQDYI
                     KVIDTFKHDVSFFTVNISSPNTENLQNFHDKDEFSMLCQALTAFKKQHDVTVPIYLKL
                     TSDMDFDGLKALLPAITETFDGIILANTTRQRDGLTSANKVEEGGLSGRPLFERNLKL
                     IKYAYQQTNGEFLIIGTGGVFSTEDAIKMMRHGASLIQIYSSLVIEGPGLTKKMNKGI
                     ARYLKDHHFDNVSDIIGLDA"
     gene            1384..2250
                     /locus_tag="O2701_000888"
     CDS             1384..2250
                     /locus_tag="O2701_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCZ4292793.1"
                     /translation="MNEQWLEHLPLKDIKEISPVSGGDVNEAYRVETDTDTFFLLVQR
                     GRKESFYAAEIAGLNEFERAGITAPRVIASGEVNGDAYLVMTYLEEGASGSQRQLGQL
                     VAQLHSQQQEEGKFGFSLPYEGGDISFDNHWQDDWCTIFVDKRLDHLKDELLNRGLWD
                     ANDIKVYDKVRRQIVAELEKHQSKPSLLHGDLWGGNYMFLQDGRPALFDPAPLYGDRE
                     FDIGITTVFGGFTSEFYDAYNKHYPLAKGASYRLEFYRLYLLMVHLLKFGEMYRDSVA
                     HSMDKILQDTTS"
     gene            2592..2870
                     /locus_tag="O2701_000889"
     CDS             2592..2870
                     /locus_tag="O2701_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04197 family type VII secretion effector"
                     /protein_id="HCZ4292794.1"
                     /translation="MMSTVQSDIFKTNSASSSIKSAVETCNNVSKPDKDESTTVSGNN
                     NAHSVIDDLMSKNQSVAEAIRTASDNIQKVGEAFDQTDVMIGNEIGKN"
     gene            2885..3253
                     /locus_tag="O2701_000890"
     CDS             2885..3253
                     /locus_tag="O2701_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292795.1"
                     /translation="MSNKLDEINKIITAKHEQMDDLYDEKREVKALIDESDALNHSID
                     QLYQHLGERYYSSNMASRMEQFRDEFHFAKRRSTEALYEQQQQIQHGIRKVEEEMIDL
                     EMRRNVEIETVTKEENKWKQ"
     gene            3241..3537
                     /locus_tag="O2701_000891"
     CDS             3241..3537
                     /locus_tag="O2701_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HCZ4292796.1"
                     /translation="METIGSIIYLKEGSQKLMIINRGPIVEIENQKYMFDYSACKYPI
                     GVVEDEIYYFNEENIDSVIFKGYSDQDEVRFQELFENMKQNLDSEIQRGEVTQQ"
     gene            3871..4977
                     /locus_tag="O2701_000892"
     CDS             3871..4977
                     /locus_tag="O2701_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HCZ4292797.1"
                     /translation="MQHHKVAIIGAGAAGIGMAITLKDFGITDVIILEKGTVGHSFKH
                     WPKSTRTITPSFTSNGFGMPDMNAISMDTSPAFTFNEEHISGETYAEYLQVVANHYEL
                     NIFENTVVTNISVDDAYYTIATTTEIYHADYIFVATGDYNFPKKPFKYGIHYSEIEDF
                     DNFNKGQYVVIGGNESGFDAAYQLAKNGSDIALYTSTTGLNDPDADPSVRLSPYTRQR
                     LGNVIKQGARIEMNVHYTVKDIDFNNGQYHISFDSGQSVLTPHEPILATGFDATKNPI
                     VQQLFVTTNQDIKLTTHDESTRYPNIFMIGATVENDNAKLCYIYKFRARFAVLAHLLT
                     QREGLPAKQDVIENYQKNQMYLDDYSCCEVSCTC"
     gene            4971..6311
                     /locus_tag="O2701_000893"
     CDS             4971..6311
                     /locus_tag="O2701_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HCZ4292798.1"
                     /translation="MLEVKYDMRTGHYTPIPNEPHYLVISHADKLTATEKAKLRLLII
                     KQKLDISLAESVVSSPIASEHVIEQLTLFQHERRHLRPKISATFLAWLLIFLMFALPI
                     GIAYQFSDWFQNQYVSAWIEYLTQTTLLNHDILQHILFGDYGVLSLGTYSLVWALPVV
                     ILISLSTAIIDQTGLKSWMIWAIEPSMLWIGLQGNDIVPLLEGFGCNAAAISQAAHQC
                     HTCTKTQCMSLISFGSSCSYQIGATLSIFSVAGKSWLFMPYLILVLLGGILHNRIWLK
                     KNDQQLSVPLPYDRQLHMPNIRQMLLQMWQNIQMFIVQALPIFITICLIVSILSLTPI
                     LNVLSQIFTPILSLLGISSELSPGILFSMIRKDGMLLFNLHQGALLQGMTATQLLLLV
                     FFSSTFTACSVTMTMLLKHLGGQSALKLIGKQMVTSLSLVIGVGIIVKIVMLII"
     gene            6409..7299
                     /locus_tag="O2701_000894"
     CDS             6409..7299
                     /locus_tag="O2701_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCZ4292799.1"
                     /translation="MKIVIIGGFLGGGKTTVLNHLLAESLKESLKPAVIMNEFGKMSV
                     DGALVSEDIPLSELTEGCICCAMKADVSEQLHQLYLKEQPDIVFIECSGIAEPVSVLD
                     ACLTPILAPFTTITHMIGVIDASMYKHIKSFPKDIQGLFYEQLAYCSVLFVNKIDSAD
                     VETTSKLLKDLEVINPEADIQVGMHGSVTLPISVRQMTATSDNKHKSLHQMINHQFVQ
                     SPVKCTKAEFIKRLACLPSHIYRLKGFMTFEDTAHTYLIQFTQGQYELTPVAFSKKVP
                     EYLVLIGKGISKEDYQCLEQ"
     gene            complement(7446..8276)
                     /locus_tag="O2701_000895"
     CDS             complement(7446..8276)
                     /locus_tag="O2701_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCZ4292800.1"
                     /translation="METLELQGAKLRYHQVGQGPVLIFIPGANGTGDIFLPLAEQLKD
                     HFTVVAVDRRDYGESELTEPLPDSASNPDSDYRVKRDAQDIAELAKSLSDEPVYILGS
                     SSGSIVAMHVLKDYPEVVKKIAFHEPPINTFLPDSTYWKDKNDDIVHQILTEGLEKGM
                     KTFGETLNIAPIDAKMMSQPADTEEGRIEQYKRTMFWLEFEIRQYTHSNITLDDFTKY
                     SDKITLLNGTDSRGSFPQDVNFYINKETGIPIVDIPGGHLGYIQKPEGFADVLLNMWG
                     "
     gene            complement(8296..9306)
                     /locus_tag="O2701_000896"
     CDS             complement(8296..9306)
                     /locus_tag="O2701_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCZ4292801.1"
                     /translation="MKSITFEEHYVIEDIQKETMNAISADPKGVPMKVMLEGLEKKTG
                     FTNADELSHHDERIQFMNNQDVQIQVLSYGNGSPSNLVGQKAIELCQKANDQLANYIA
                     QYPNRFVGFATLPINEPEAAAREFERCINDLGFKGALIMGRAQDGFLDQDKYDIIFKT
                     AENLDVPIYLHPAPVNSDIYQSYYKGNYPEVTAATFACFGYGWHIDVGIHAIHLVLSG
                     IFDRYPKLNMIIGHWGEFIPFFLERMDEALFAEHLNHPVSYYFKNNFYITPSGMLTKP
                     QFDLVKKEVGIDRILYAADYPYIEPEKLGVFLDELGLTDEEKEKISYTNGAKLLGLSP
                     NN"
     gene            complement(9404..10108)
                     /locus_tag="O2701_000897"
     CDS             complement(9404..10108)
                     /locus_tag="O2701_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCZ4292802.1"
                     /translation="MTKIVLITGGNKGLGYASAEALKALGYKVYIGSRNDVRGQQASQ
                     KLGVHYVQLDVTSDYSVKNAYNMIAEKEGRLDILINNAGISGQFSAPSKLTPRDVEEV
                     YQTNVFGIVRMMNTFVPLLEKSEQPVVVNVSSGLGSFGMVTNPETAESKVNSLAYCSS
                     KSAVTMLTLQYAKGLPNMQINAADPGATNTDLVGDFSNNSKHVSEGIKPIIQLATIGA
                     DGPTGTFINGDGEMPW"
     gene            complement(10101..10658)
                     /locus_tag="O2701_000898"
     CDS             complement(10101..10658)
                     /locus_tag="O2701_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCZ4292803.1"
                     /translation="MRKDAKENRQRIEEIAHKLFDEEGVENISMNRIAKELGIGMGTL
                     YRHFKDKSDLCYYVIQRDLDIFITHFKQIKDDYHSNYEVMQVSLDYLLQFKIDNKALL
                     QCIEAGNNKLRFYQSAFYQELFDFYYDLFKSDDDTYTKFKTDMLLQSLSTSVFAFQIE
                     HRHISIEAYRNYLLNIYLDEVGRND"
     gene            10789..11088
                     /locus_tag="O2701_000899"
     CDS             10789..11088
                     /locus_tag="O2701_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HCZ4292804.1"
                     /translation="MSLNKEQRRITAEELQAHFEASTLSVQMIAEKLNVTTEDVEKVL
                     AMTAPLGIFSHQLQRFIHLVWDVRDVINDNIKGNGQTPEPYTYLKGEKEDYWFLR"
     gene            11108..11929
                     /locus_tag="O2701_000900"
     CDS             11108..11929
                     /locus_tag="O2701_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HCZ4292805.1"
                     /translation="MKDILVIGATGKQGNAVVKQLLEDGWYVSALTRNKNNRKLSDIG
                     HPHLSIVEGDLSDNVSLQSAMKGKYGLYSIQPIVKDDVSEELRQGMKIIEIAEQENIQ
                     HIVYSTAGGVNRNRTGPHFEVLAKIENRLMESNINATVIKPSFFMDNFLRIAKVEDER
                     ITLPEFINPNIKFTMISSIDIAKIASYIFAHPQSFTHQSIEIGSDEVTLSEAATIFSE
                     VTGKSTVIEGEFVSGVAEKQWLEEKGYEVDFELMAEINPTRLSLSDWLKVQNYNK"
     gene            11946..12323
                     /locus_tag="O2701_000901"
     CDS             11946..12323
                     /locus_tag="O2701_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCZ4292806.1"
                     /translation="MIQSMWFNLHVQDLEKSAQFYKALGFKINRNPQMLDKMVGIQIG
                     QTTVILIENKHFQNVSQQSLNTEPNEVMISLGVNTNEEVDQLVNKVKEAGGTVVQEPT
                     VSQGFYGAMFKDLDGHHFNFLVC"
     gene            complement(12473..12703)
                     /locus_tag="O2701_000902"
     CDS             complement(12473..12703)
                     /locus_tag="O2701_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HCZ4292807.1"
                     /translation="MKILDRINELANKEKVQPLTVAEKQEQHALRQDYLSMIRGQVLT
                     TFSTIKVVDPIGQDVTPDKVYDLRQQYGYIQN"
     gene            complement(12923..13423)
                     /locus_tag="O2701_000903"
     CDS             complement(12923..13423)
                     /locus_tag="O2701_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292808.1"
                     /translation="MTSIFVFAYLNNTSGNMLFRQQMICACLLANVRHSSIVCLNNIC
                     CIVDMRCLNTKIGGIDVSETDEPQGFERTHNILNINQSSLGVVTYITNKLKSTLKQHI
                     IIARGKKRIDYRLSYNFYTTYYDNVEIKKIDCEYTYAMPIAILIRHTMSFDNAHFFAQ
                     LRHPVL"
     gene            complement(13621..14190)
                     /locus_tag="O2701_000904"
     CDS             complement(13621..14190)
                     /locus_tag="O2701_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCZ4292809.1"
                     /translation="MMESKSIDPRIVRTKQLLVDAFLKISREKKLSQITVKDITDIAT
                     LNRATFYAHFTDKEDLLDYTLSVTILKDLNDNLSISNVINEKVLRNIFISIASYIKDA
                     AKSCELNSEAFCNKAHQRINNELEDIFAIMLENSYPEHQRDIIVNSASFLAAGISGLA
                     LHWFNTSQETADVFIDRNLPFLIHHIAHF"
     gene            14345..14473
                     /locus_tag="O2701_000905"
     CDS             14345..14473
                     /locus_tag="O2701_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292810.1"
                     /translation="MDFKNEDKIKVYCNNMFEKLPISLSENSEFCDILFFIVLSYR"
     gene            14600..15655
                     /locus_tag="O2701_000906"
     CDS             14600..15655
                     /locus_tag="O2701_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HCZ4292811.1"
                     /translation="MTSIKTITPKDFIFRVLSGVAIGIVAGLVPNAILGEIFKYFMDY
                     HPIFKTLLGVVVAIQFTVPALIGALVAMKFDLSPLAIAVVASAAYVGSGAAQFKNGAW
                     MITGIGDLINTMITAAIAVLFILLIQHRVGSMALIVFPTVVGGISGAIGVLILPYTKM
                     ITTAIGNMVNGFTELQPIVMSILISMVFSLIIISPLSTVAIAFAIGITGLAAGSASIG
                     ISATEAVLIIGTSKVNRLGVPLSVFFGGVKMMIPNMVKYPILMLPILTTAIVSGLVSA
                     LVGIHGTKESAGFGFIGMVGPINAFKFMEVDSAWLSVLLIVVAFFVVPFVTAWLADII
                     YRKVFRLYTNDIFKFMG"
     gene            16263..16964
                     /gene="isaA"
                     /locus_tag="O2701_000907"
     CDS             16263..16964
                     /gene="isaA"
                     /locus_tag="O2701_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase IsaA"
                     /protein_id="HCZ4292812.1"
                     /translation="MKKTIMASSLAVALGVTGYAAGTGHQAHAAEVNVDQAHLVDLAH
                     NHQDQLNAAPIKDGAYDIHFVKDGFQYNFTSNGTTWSWSYEAANGQTAGFSNVAGADY
                     TTSYNQGSNVQSVSYNAQSSNSNVEAVSAPTYHNYSTSTTSSSVRLSNGNTAGATGSS
                     AAQIMAQRTGVSASTWAAIIARESNGQVNAYNPSGASGLFQTMPGWGPTNTVDQQINA
                     AVKAYKAQGLGAWGF"
     gene            17240..17728
                     /locus_tag="O2701_000908"
     CDS             17240..17728
                     /locus_tag="O2701_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4292813.1"
                     /translation="MAINIIEYNRSYKEELIEFILSIQKNEFNIKIDRDDQPDLENIE
                     HNYLNNGGQFWLAINNHQNIVGTIGLIRLDNNMSALKKMFVDKGYRNLKIGKKLLDKV
                     IMTCKEQNIDGIYLGTIDKFISAQYFYSNNGFREIKRGDLPSSFPKLDVDNRFYYRNL
                     KD"
     gene            18239..20050
                     /locus_tag="O2701_000909"
     CDS             18239..20050
                     /locus_tag="O2701_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCZ4292814.1"
                     /translation="MDTKDFKRLEKMYSPRYLPGLDGLRAFAVIGIIIYHLNAQWLSG
                     GFLGVDTFFVISGYLITSLLISEYYRTQKIDLLEFWKRRLKRLIPAVLFLICVVLTFT
                     LIFKPELIIQMKRDAIAAIFYVSNWWYISQNVDYFNQFAIEPLKHLWSLAIEEQFYLL
                     FPLVITFLLHRFKPRNIIQTLFIVSLISLGLMIVIHFITGDNSRVYFGTDTRLQTLLL
                     GCILAFIWPPFALKKDISKKIVVSLDIIGISGFAVLMTLFFIVGDQDQWIYNGGFYII
                     SFATLFIIAIAVHPSSLFAKFLSMKPLLIIGKRSYSLYLWHYPIIVFVNSYYVQGQIP
                     VYVYIIEILLTALMAEISYRFIETPIRKKGFKAFAFLPKKKGQFARTVLVILLLVPSI
                     VVLSGQFDALGKQHEAEKKEKKTEFKTTKKKVVKKDKQEDKQTANSKEDIKKSSPLLI
                     GDSVMVDIGNVFTKKIPNAQIDGKVGRQLVDATPIVKSQYKDYAKKGQKVVVELGTNG
                     AFTKDQLNELLDSFGKADIYLVSIRVPRDYEGRINKLIYEAAEKRSNVHLVDWYKASA
                     GHPEYFAYDGIHLEYAGSKALTDLIVKTMETHATNKK"
     gene            20411..21178
                     /locus_tag="O2701_000910"
     CDS             20411..21178
                     /locus_tag="O2701_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCZ4292815.1"
                     /translation="MKKIVTATIATAGLATIAFAGHDAQAAEQNNNGYNSNDAQSYSY
                     TYTIDAQGNYHYTWTGNWNPSQLTQNNTYYYNNYNTYSYNNASYNNYYNHSYQYNNYT
                     NNSQTATNNYYTGGSGASYSTTSNNVHVTTTAAPSSNGRSISNGYASGSNLYTSGQCT
                     YYVFDRVGGKIGSTWGNASNWANAAASSGYTVNNTPKVGAIMQTTQGYYGHVAYVEGV
                     NSNGSVRVSEMNYGHGAGVVTSRTISANQAGSYNFIH"
     gene            21353..21841
                     /gene="crtO"
                     /locus_tag="O2701_000911"
     CDS             21353..21841
                     /gene="crtO"
                     /locus_tag="O2701_000911"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl-4,4'-diaponeurosporenoate
                     acyltransferase"
                     /protein_id="HCZ4292816.1"
                     /translation="MKKYIKTAFFCSMYWLIVQLNIANLGTRIPDKYFRQKYIIFKSF
                     NFEKHGKFWNKWFYVRKWKHKILDGHQLNQNIYDQRHLMTINTDEIEKMIIETKRAEL
                     IHWISILPVIIFNKGPRLVKYINIFYAMIANVPIIIVQRYNRPRLTQLLRILKRRGER
                     HD"
     gene            21834..23327
                     /locus_tag="O2701_000912"
     CDS             21834..23327
                     /locus_tag="O2701_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCZ4292817.1"
                     /translation="MTKHIIVIGGGLGGISAAIRMAQSGYSVSLYEQNNHIGGKVNRH
                     ESDGFGFDLGPSILTMPYIFEKLFEYSKKQMSDYVTIKRLPHQWRSFFPDGTTIDLYE
                     GIKETGQHNAILSKQDIEELQNYLNYTRRIDRITEKGYFNYGLDTLSQIIKFHGPLNA
                     LINYDYVHTMQQAIDKRISNPYLRQMLGYFIKYVGSSSYDAPAVLSMLFHMQQEQGLW
                     YVEGGIHHLANALEKLAREEGVTIHTGARVDNIKTYQRRVTGVRLDTGEFVKADYIIS
                     NMEVIPTYKYLIHLDTQRLNKLEREFEPASSGYVMHLGVACQYPQLAHHNFFFTENAY
                     LNYQQVFHEKVLPDDPTIYLVNTNKTDHTQAPVGYENIKVLPHIPYIQDQPFTTEDYA
                     KFRDKILDKLEKMGLTDLRKHIIYEDVWTPEDIEKNYRSNRGAIYGVVADKKKNKGFK
                     FPKESQYFENLYFVGGSVNPGGGMPMVTLSGQQVADKINAREAKNRK"
     gene            23333..24460
                     /gene="crtQ"
                     /locus_tag="O2701_000913"
     CDS             23333..24460
                     /gene="crtQ"
                     /locus_tag="O2701_000913"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,4'-diaponeurosporenoate glycosyltransferase"
                     /protein_id="HCZ4292818.1"
                     /translation="MKWLSRILTVIVTMSMACGALIFNRRHQLKAKTLNFNHKALTII
                     IPARNEEKRIGHLLHSIIQQQVPVDVIVMNDGSTDETARVARSYGATVVDVVDDTDGK
                     WYGKSHACYQGVTHACTNRIAFVDADVTFLRKDAVETLINQYQLQGEKGLLSVQPYHI
                     TKRFYEGFSAIFNLMTVVGMNVFSTLDDGRTNQHAFGPVTLTNKEDYYATGGHKSANR
                     HIIEGFALGSAYTSQSLPVTVYEGFPFVAFRMYQEGFQSLQEGWTKHLSTGAGGTKPK
                     IMTAIVLWLFGSIASILGLCLSLKYRQMSVRKMVALYLSYTTQFIYLHRRVGQFSNLL
                     MVCHPLLFMFFTKIFIQSWKQTHRYGVVEWKGRQYSISKEQ"
     gene            24497..25360
                     /locus_tag="O2701_000914"
     CDS             24497..25360
                     /locus_tag="O2701_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HCZ4292819.1"
                     /translation="MTMMDMNFKYCHKIMKKHSKSFSYAFDLLPEDQRKAVWAIYAVC
                     RKIDDSIDVYGDIQFLNQIKEDIQSIEKYPYEHHHFQSDRRIMMALQHVAQHKNIAFQ
                     SFYNLIDTVYKDQHFTMFETDAELFGYCYGVAGTVGEVLTPILSDHETHQTYDVARRL
                     GESLQLINILRDVGEDFDNERIYFSKQRLKQYEVDIAEVYQNGVNNHYIDLWEYYAAI
                     AEKDFQDVMDQIKVFSIEAQPIIELAARIYIEILDEVRQANYTLHERVFVDKRKKAKL
                     FHEINSKYHRI"
     gene            25372..26880
                     /locus_tag="O2701_000915"
     CDS             25372..26880
                     /locus_tag="O2701_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCZ4292820.1"
                     /translation="MKIAVIGAGVTGLAAAARIASQGHEVTIFEKNNNVGGRMNQLKK
                     DGFTFDMGPTIVMMPDVYKDVFTACGKNYEDYIELRQLRYIYDVYFDHDDRITVPTDL
                     AELQQMLESIEPGSTHGFMSFLTDVYKKYEIARRYFLERTYRKPSDFYNMTSLVQGAK
                     LKTLNHADQLIEHYIDNEKIQKLLAFQTLYIGIDPKRGPSLYSIIPMIEMMFGVHFIK
                     GGMYGMAQGLAQLNKDLGVNIELNAEIEQIIIDPKFKRADAIKVNGDIRKFDKILCTA
                     DFPSVAESLMPDFAPIKKYPPHKIADLDYSCSAFLMYIGIDIDVTDQVRLHNVIFSDD
                     FRGNIEEIFEGRLSYDPSIYVYVPAVADKSLAPEGKTGIYVLMPTPELKTGSGIDWSD
                     EALTQQIKEIIYRKLATIEVFEDIKSHIVSETIFTPNDFEQTYHAKFGSAFGLMPTLA
                     QSNYYRPQNVSRDYKDLYFAGASTHPGAGVPIVLTSAKITVDEMIKDIERGV"
     gene            27307..28461
                     /locus_tag="O2701_000916"
     CDS             27307..28461
                     /locus_tag="O2701_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCZ4292821.1"
                     /translation="MISNKLANIPDSYFGKTMGRKIEHGPLPLINMAVGIPDGPTPQG
                     IIDHFQKALTIPENQKYGAFHGKEAFKQAIVDFYQRQYNVTLDKEDEVCILYGTKNGL
                     VAVPTCVINPGDYVLLPDPGYTDYLAGVLLADGKPVPLNLEPPHYLPDWSKVDSQIID
                     KTKLIYLTYPNNPTGSTATKEVFDEAIAKFKGTDTKIVHDFAYGAFGFDAKNPSILAS
                     ENGKDVAIEIYSLSKGYNMSGFRVGFAVGNKDMIQALKKYQTHTNAGMFGALQDAAIY
                     ALNHYDDFLEEQSNVFKTRRDRFEAMLAKADLPFVHAKGGIYVWLETPPGYDSEQFEQ
                     FLVQEKSILVAPGKPFGENGNRYVRISLALDDQKLDEAAIRLTELAYLYE"
     gene            28484..29482
                     /locus_tag="O2701_000917"
     CDS             28484..29482
                     /locus_tag="O2701_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCZ4292822.1"
                     /translation="MTKIKIMSVRDEDMPYIKAWAEKHHVEVDITKEALTDDNVEGVA
                     GYDGLSLSQQIPLSEHVYKRLNELGIKQIAQRSAGFDTYDLELANKYNFIVSNVPSYS
                     PNSIAEFAVNQAINVVRHFNQIQTKVREHDFRWEPTILSKSIKDLKVAVIGTGRIGRV
                     VADIFANGYQSDVVAYDPFPNAKIATYVDYKDTIEEAVEGADIVTLHVPATKYNHYLF
                     NAELFKHFKKGAVFVNCARGSLVDTKALLDALDNGVIKGAALDTYEFERKLFPSDQRG
                     KTLNDPLLESLIDREDVILTPHIAFYTEAAVKNLIVDALDATLDVLQTGDTRLRVN"
     gene            complement(29572..29778)
                     /gene="copZ"
                     /locus_tag="O2701_000918"
     CDS             complement(29572..29778)
                     /gene="copZ"
                     /locus_tag="O2701_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HCZ4292823.1"
                     /translation="MSQEILNVEGMSCGHCKSAVESALNNIDGVTSADVNLENGQVSV
                     QYDDSKVAVSQMKDAIEDQGYDVV"
BASE COUNT        10089 a         4489 c         5584 g         9649 t
ORIGIN      
        1 cattattttg aaaaatggtg ataaatatat cttcttgagt tatatcataa ttttaataag
       61 acgattcagc aagttttaaa gtattatttg actatgttgg attaggcatc tagtcctata
      121 atatcactga cattgtcaaa atgatgatct tttaagtaac gtgcgatgcc tttgttcatt
      181 ttcttagtta aacctgggcc ttcaataaca agtgatgaat aaatttgaat aagtgacgca
      241 ccgtgacgca tcattttgat tgcatcttca gtactgaata cgccgcctgt acctataatt
      301 aaaaattcac catttgtttg ctgataagca tacttaatca attttaaatt acgttcaaat
      361 aatggacgac cactcaaacc gccttcttcg actttattag cagaagttaa accatctcgt
      421 tgtcgcgttg tgtttgctaa gatgataccg tcaaatgtct cagtaatcgc tggtaatagt
      481 gcttttaagc catcgaaatc catatcagac gttagtttta aataaattgg cactgttaca
      541 tcatgttgtt ttttaaatgc tgttaaagct tggcataaca ttgaaaattc atctttatca
      601 tggaagtttt gaagattttc agtatttgga gaactgatgt tgactgtgaa aaatgaaacg
      661 tcgtgtttaa acgtatcaat aacctttata taatcttgat aacgcgcttc ataaggtgtc
      721 attttattca caccaacatt gataccaaca ggtacttgat aagcattttt acgcaaatga
      781 cttagtgctt tgttcatacc aatattattg aagcccattc gatttatcaa ggcgtcatct
      841 tctaataatc taaacatgcg tggttgaggg ttacccggtt gaggtttagg tgtgatacca
      901 cctaattcta aagcaccgaa tccaaggtgt tccaatgctt ttggtacttc gcaagatttg
      961 tcgaaaccag ctgctaagcc aattggattg tcgtacgtat taccttgtat cgtttgtgat
     1021 aacgttggat tcttataagt aaatagttta tcgacgactg ggaataaaac cggaaacttt
     1081 tgtaacgttt ttaatgcatc gatagttagt ccgtgtgctt tttcgggttc gattttgaat
     1141 aagaaaggtt taattaattt gtacatgagt atgctcctat ttcattatat ttgaggctta
     1201 ctatcctcaa cttaatatat gtgaaatata ttcttttaat agactagcat ttccatacat
     1261 aatttcctag ttaaaactaa aaagttttga aaattgacgc aagtttgaat aacgttttta
     1321 agattaaatc atcctaatta ggcaatatta tagtataaag taagtagatt ggaaggtgtt
     1381 tgtatgaatg aacaatggtt agagcattta cctttaaaag atattaaaga gatttcacca
     1441 gtgagtggtg gtgatgtaaa cgaagcatat cgagtcgaaa cagatacgga tacatttttc
     1501 ttacttgtcc aacgtggacg taaagaatca ttttatgctg cagaaattgc aggtttaaat
     1561 gaatttgaac gtgcaggtat cacggcacct agagtaattg caagtggcga ggttaacggt
     1621 gatgcgtatt tagtgatgac gtatttagaa gaaggggctt cagggagtca acgccaatta
     1681 gggcaactcg tagctcaatt acacagtcag caacaagaag aaggcaaatt tggcttctca
     1741 ttaccttatg aaggtggcga tatttctttt gataatcatt ggcaagacga ttggtgtacc
     1801 atttttgtcg acaaacgctt ggaccatttg aaagatgagt tgctaaatag agggctatgg
     1861 gatgccaacg atatcaaagt atatgacaaa gtgcgacgtc aaattgtggc ggaattagaa
     1921 aagcatcaaa gtaaaccgtc tttattacat ggtgacctat ggggtggtaa ttatatgttc
     1981 ttacaagatg gtcgtccggc gttatttgat ccagcgccat tatatggtga cagagaattc
     2041 gatatcggta ttacaacggt atttggtggt tttacgagcg aattttatga tgcgtataat
     2101 aaacattatc cactcgcaaa aggtgcatcc tatagacttg aattttatcg tttatattta
     2161 ttgatggtcc atttattgaa atttggtgag atgtaccgtg atagtgttgc gcattctatg
     2221 gataagattt tacaagatac aacaagttag ttaagacgtt agattgagat aaatagataa
     2281 tatgcacaga tatttttaca atgagaagcg atacagctgc ctcaataaaa atatttgtgc
     2341 gtttttattg ttggaaaata aaattttaat cgctattgtt aatttctgta atgtaaaaca
     2401 aggttgagtt acaataaaag tgattttata actttttgtt caataaaatt ctaggaatga
     2461 tacatattta ttgatacaat aattttgaat ataatcataa aacaatattt aagtataatt
     2521 gaatgtttga atatcatata ttgatacagt ttctaataat tttaaaataa tttaaatgga
     2581 gagaggtgta aatgatgagt acagttcaaa gtgatatttt taagaccaat agtgcatcat
     2641 catctattaa aagcgctgtt gaaacatgta ataatgtgtc gaaaccggat aaagatgaaa
     2701 gtacaacagt aagtggaaat aataatgctc atagtgtgat agatgatttg atgagtaaga
     2761 atcaatctgt tgctgaagca atacgaactg cgagcgataa tatacaaaaa gttggtgagg
     2821 cttttgacca aactgacgta atgattggta atgaaattgg taaaaattaa aacgtggtga
     2881 aatgatgtcg aataaactgg atgaaatcaa taaaataatc acagcgaaac atgagcaaat
     2941 ggatgactta tatgatgaaa agcgagaggt taaagcattg atagatgaaa gtgatgcgct
     3001 taatcattcg atagatcaat tatatcaaca tttaggtgag cgttattata gtagcaatat
     3061 ggctagtcgt atggaacagt tccgcgatga atttcatttt gcgaaacgac gttcaacgga
     3121 agcgttatac gagcagcaac agcaaattca acatggcatt cgtaaagtgg aagaagagat
     3181 gattgacttg gaaatgcgaa ggaatgttga aattgagacg gtgacaaagg aggaaaataa
     3241 atggaaacaa taggaagcat tatttattta aaagaaggtt cgcaaaagtt aatgattatt
     3301 aatagaggac caattgtaga aattgaaaat caaaagtata tgtttgacta ttctgcatgt
     3361 aaatatccga ttggtgttgt agaagatgaa atttattatt ttaacgagga aaatatagat
     3421 tcagttattt ttaaaggtta ttctgatcaa gatgaggtta gatttcaaga gttgtttgaa
     3481 aatatgaaac aaaatttgga tagtgaaata caacgtggag aagttacaca acaataaaga
     3541 aatacttttt ctttattggg gtgggacgac gaaataaatt ttgtaaaaat atgatttctg
     3601 tctcaccgcc aaaatgttat ctccaagtac tgaggctgga tgataccatg aaatgtcatt
     3661 cataatcatt tgaactaaac gtagcagcct taaattttaa aaaaagacac ataccaactt
     3721 ccgaaatgta gatgaattct ctacaataac ggaagttttt cttttaatat tgaaatttct
     3781 caaggatagg tctatacttt ataaatcgta attattacga tttataatca aaaacaataa
     3841 cttgaaatag atcattgagg gagtgttaat atgcaacatc ataaagtggc tattatcggt
     3901 gccggtgctg caggtatagg tatggccatt accttaaaag atttcggtat aacagatgtc
     3961 attattttag aaaaaggaac agtaggacat tcatttaaac attggccgaa atcgacccgt
     4021 acgatcacgc catcatttac gtctaatgga tttggcatgc ctgatatgaa tgcaatttcc
     4081 atggatactt caccagcatt tacatttaat gaagaacata tttccggaga aacatatgct
     4141 gaatatttac aagtggttgc caaccattac gagctgaata tctttgaaaa tacagttgtc
     4201 acaaatatat ctgtagatga tgcatattat acgattgcaa cgacaacaga gatatatcac
     4261 gcggattata tctttgtcgc aacaggtgat tataatttcc ctaaaaagcc atttaaatat
     4321 ggtattcatt atagtgaaat tgaagacttt gataacttta ataaggggca atatgtggtt
     4381 atcggaggta atgaaagtgg ctttgatgct gcatatcaac ttgcaaaaaa tggctctgac
     4441 atcgcacttt atactagcac aaccggttta aatgatccgg atgctgatcc tagtgttaga
     4501 ttgtcacctt atacacgtca gcgactaggt aatgtcatta agcaaggtgc tcgcatcgaa
     4561 atgaatgtac attatacagt taaagatatt gattttaaca atggacagta tcatatcagt
     4621 tttgatagcg gacaaagtgt gcttacacct catgaaccaa tactagcaac tggctttgat
     4681 gcaacaaaaa atccaatcgt tcaacaatta tttgtgacaa caaatcaaga tattaaatta
     4741 acaacacatg atgaatcgac acgttatccg aatattttta tgattggtgc aacagttgaa
     4801 aatgataatg ccaaattatg ctatatctat aaatttagag cgcgatttgc agtacttgca
     4861 catcttttaa cacagcggga aggcttacca gctaaacaag atgtcattga aaattatcaa
     4921 aaaaatcaaa tgtatttaga tgattattca tgttgtgaag tgtcatgcac atgttagaag
     4981 tgaaatatga tatgagaact gggcattata cgcccatacc taatgaacct cattatttgg
     5041 ttattagtca tgcggataaa cttaccgcaa cagaaaaagc gaaattaaga ttattaatca
     5101 taaaacagaa attagatatt tcattggcag aaagtgtagt ttcttcgcct atagcgagtg
     5161 aacatgtgat agaacaattg acactatttc aacatgagcg acgacattta agacctaaaa
     5221 taagtgcgac atttttagcc tggttgttga tatttttaat gtttgcattg ccaatcggta
     5281 tcgcttatca attttcagat tggtttcaaa atcagtatgt gtcagcatgg atagaatatt
     5341 taactcaaac aacattgctc aatcacgata tattacagca tatattattt ggtgattatg
     5401 gtgtgctatc acttggaaca tattcgctcg tatgggcatt gccggttgta atattgatta
     5461 gtttatcaac tgctataatt gatcaaacag gactcaaatc atggatgata tgggcaattg
     5521 aaccgtcaat gttatggata ggattacaag gtaatgatat cgtgccacta ttagaagggt
     5581 ttggatgtaa tgcagcagct atttcacaag cagcacacca atgccatacc tgcacgaaga
     5641 cacagtgtat gagtttaata agctttggta gttcttgtag ttatcaaata ggtgcgacat
     5701 tatctatttt tagtgtagct ggaaagtcat ggctatttat gccgtactta atattagtac
     5761 ttttaggtgg catcttacat aataggatat ggttgaaaaa gaatgatcaa caacttagcg
     5821 ttccgctacc ttatgatagg caattacata tgccaaatat acgtcaaatg ttgctacaaa
     5881 tgtggcaaaa tatacaaatg tttatcgttc aagcgctacc tatttttatc acaatctgtc
     5941 ttattgttag tattttatca ctaacgccaa ttttgaatgt tttatcacaa atatttacac
     6001 ctatattatc gttattaggc atctcgtcag aattgtcacc agggatttta ttttcaatga
     6061 ttcgaaaaga cggcatgctc ttgtttaatt tgcatcaggg cgccttatta caaggaatga
     6121 cagcaacaca gttactacta cttgtgtttt ttagttcaac atttacagcg tgctcggtca
     6181 caatgacgat gcttttgaaa catttaggtg gtcagtcagc actaaaatta attggaaagc
     6241 aaatggtgac atcattgtct ttagttattg gtgtaggcat cattgttaaa atagtaatgc
     6301 tgattattta aaaaaaatga actataactg aatatagagt catgtcagtc aataggagat
     6361 ctatcttgga atatgctatt catatgaagt ataagaggag agtcgcagat gaaaatagtt
     6421 attataggtg ggtttttagg tggcggtaaa acgactgtct taaatcattt gctcgctgaa
     6481 tcattaaagg aatcgctgaa accagcagtc atcatgaatg aatttgggaa aatgagtgtt
     6541 gatggtgcct tagtatctga agacatacct ttaagtgaac tgacagaggg gtgtatctgt
     6601 tgtgcaatga aagcagatgt atcagaacag ttacatcaat tatatttaaa agagcaacca
     6661 gacattgtat ttattgaatg tagtgggatt gcagaaccgg tctctgtctt agatgcttgt
     6721 ttaacgccta ttttagctcc gtttacaaca attacacata tgattggtgt aatagacgca
     6781 agcatgtata aacacattaa atcattccct aaagacatcc aaggcttatt ttatgagcaa
     6841 ttagcatatt gttctgtctt atttgttaat aaaatagatt cagcagatgt tgaaacaacg
     6901 agcaaactat tgaaagattt agaagttatt aacccagagg ccgatataca agtcggtatg
     6961 catggcagcg tcactttgcc aatatcagtt agacaaatga cagcaacttc tgacaataaa
     7021 cataagtctt tacatcaaat gattaatcat caatttgtgc aatcaccagt caaatgtact
     7081 aaagcagagt ttataaaacg tttagcatgc cttccgtctc atatttatag gttgaaaggg
     7141 tttatgacat ttgaagacac cgcacatacg tatctcattc aatttacaca aggacaatat
     7201 gaattaacac ctgtagcatt ttcaaaaaaa gtgccagaat atttggtgct tatcggaaag
     7261 ggtatttcaa aagaagacta tcaatgtttg gaacagtagt gttttcagtg gaagagaatg
     7321 gttaacatgc cttcatgtat aataacgagt tgatttgaac gtttaagcgt aaataaaaat
     7381 aagcttggtc agccatcaaa tataatttga aaactgtcca agctgtttta ttagagaaca
     7441 atcaattaac cccacatatt taataataca tcagcaaagc cttcaggttt ttgaatataa
     7501 cctaagtgac cgcctggaat atctacaata ggtatgccag tttctttatt tatataaaag
     7561 ttaacatctt gtgggaagga gcctctagaa tctgtcccat ttagtagggt gattttatcg
     7621 ctgtattttg tgaaatcatc caaagtaata tttgaatgcg tatattgtct aatttcaaat
     7681 tctaaccaga acatcgtacg tttgtactgt tctatacgtc cttcttcagt atcagcaggt
     7741 tgagacatca tttttgcatc aattggtgcg atatttaatg tttcgccaaa tgttttcatg
     7801 cctttttcta agccttctgt taaaatttga tgcacaatgt catcattttt atctttccaa
     7861 taagtactgt ctggtaaaaa tgtattaatt ggtggttcgt gaaatgcaat ctttttaacg
     7921 acttcagggt aatcttttaa cacatgcatc gcaacgattg aacctgaact tgaacctaat
     7981 atatagacag gttcatcact taatgacttt gcaagttcgg caatgtcctg tgcgtcgcgt
     8041 ttgacacgat aatcactgtc agggtttgaa gcggaatcag ggagtggttc agttaactcg
     8101 ctttctccat aatcacgacg atcaacggct acaacagtaa aatggtcttt taactgttct
     8161 gcaagaggca gaaaaatgtc tccggtaccg tttgcaccag gaataaagat gagcacgggt
     8221 ccttgtccga cttggtggta tcgtaattta gcgccttgta attctaaagt ttccatattc
     8281 aatgacctcc atttgttaat tgttaggtga taaacctaat aatttagcac catttgtata
     8341 acttattttc tctttttctt catctgttaa acccagttca tctaaaaata cacctaattt
     8401 ttcaggctca atatatggat aatcagcagc ataaagaatt ctatcaatac ctacttcttt
     8461 cttgactaaa tcaaactgtg gcttcgttaa catgccactc ggtgtgatat aaaaattatt
     8521 tttaaagtaa tagcttacag ggtggttcaa atgttcagcg aataaagctt catccatacg
     8581 ttctaagaag aatgggataa actcacccca atgtccaata atcatattta actttggata
     8641 acgatcaaaa ataccagata atactagatg tattgcatga atgccgacat caatgtgcca
     8701 accataacca aaacaagcaa atgttgccgc agttacttca ggataatttc ctttatagta
     8761 tgattgataa atgtcactgt taactggcgc gggatgtaga taaatcggta cgtctaaatt
     8821 ttcagctgtt ttgaaaataa tgtcatattt gtcttgatca agaaaaccat cttgtgcacg
     8881 tcccataatg agcgcacctt tgaatcctaa atcattgatg caacgttcga attctcgcgc
     8941 tgcggcttca ggctcattga taggtaaagt tgcaaagcct acaaagcgat tgggatattg
     9001 tgcaatatag tttgccaatt gatcatttgc tttttgacat aattcaatgg ctttctgacc
     9061 aaccaaattt gaaggagaac catttccata agataagact tgaatttgaa cgtcttgatt
     9121 attcataaat tggatacgtt catcatgatg tgataattcg tcggcatttg taaaacctgt
     9181 ctttttttca aggccttcta acattacttt catcggtaca cctttaggat ctgctgatat
     9241 cgcattcatc gtttcttttt gaatatcttc aatgacataa tgttcttcaa acgtaatact
     9301 tttcatttac ttcgcctcca tattgtattg catgtttatt gcatctattg cagaagcatt
     9361 ttttatatac ctctaatttc aatgtttgta acataaaatt gatctaccaa ggcatctctc
     9421 catcgccatt aataaatgta cctgttgggc catctgcacc aatcgttgct aattgaatga
     9481 ttggcttgat tccttcagaa acgtgtttgg aattattact aaaatcacca actaaatcag
     9541 tatttgtagc gcctggatca gcagcattga tttgcatgtt aggtaatcct ttagcgtatt
     9601 gtagcgttag cattgttact gccgatttag acgaacaata agctaatgaa ttcactttag
     9661 attcagctgt ttcggggttt gtaaccattc caaatgaacc taaaccactt gatacgttga
     9721 cgacaacagg ttgttcagat ttttctaaga gagggacgaa tgtattcatc attcgtacga
     9781 taccgaatac attcgtttga tatacttctt caacgtcacg aggtgtcaat ttggaaggtg
     9841 ctgaaaattg accagatata cctgcattgt taatgaggat atcaagacgg ccttcttttt
     9901 cagcaatcat gttataagca tttttgactg agtagtcact tgtaacatct aattgtacat
     9961 aatgaacacc taatttttgt gatgcttgtt gtcctcttac atcattccga gaacctatat
    10021 aaactttgta acccaatgct ttaagtgcct ctgcacttgc atagcctaac cctttattgc
    10081 ctcctgtgat taacacaatt ttagtcatta cgtcccacct catctaaata aatgtttaat
    10141 aaataatttc tgtacgcttc aattgaaata tggcgatgct ctatttggaa ggcaaataca
    10201 ctagttgata atgattgcaa cagcatatct gttttgaact tcgtgtaagt gtcgtcatcg
    10261 cttttaaata agtcataata aaaatcaaat aattcttgat aaaatgcgct ttggtaaaaa
    10321 cgtaatttat tgttgcctgc ttcaatacat tgcagtagtg ccttattatc gattttaaat
    10381 tgtaaaagat aatctaacga cacttgcata acctcataat tagaatgata gtcatcttta
    10441 atttgcttaa aatgagtgat aaaaatatca aggtctcttt gtatgacgta gtagcataaa
    10501 tcgcttttat ctttgaaatg tcgatacaat gtccccatac cgatacctag ttctttagca
    10561 atacgattca tactaatgtt ttcaacgcct tcttcatcaa aaagtttgtg cgctatttct
    10621 tcaattcgtt gcctattctc ttttgcatct tttcgcatga ttacacctac ttaaaattct
    10681 ctaaaattga caaacggata actctccgtt tattataaaa cgtgttaaga aagttagcaa
    10741 tgaatttgca ataactatta aatatcataa aagaaaagag tgttgataat gtctttaaat
    10801 aaagagcaaa gacgcatcac agctgaagag ttgcaagcac atttcgaagc atctacctta
    10861 tcggttcaaa tgattgctga aaaactgaat gtcactacag aagatgtgga aaaagtatta
    10921 gctatgacag cgccactagg catttttagt catcaattac aacgatttat tcatttagta
    10981 tgggatgtca gagatgtaat aaacgacaat attaaaggaa atggacaaac accagaacca
    11041 tatacgtatt taaaaggtga aaaagaggac tattggtttt taagataaaa agaggagtgg
    11101 atctaaaatg aaagatattt tagtaatagg cgcaacaggg aagcaaggta atgcagttgt
    11161 aaaacagttg ttagaggatg gatggtatgt aagtgcatta actagaaata agaataatcg
    11221 taaactttca gacataggac atccgcattt atcaatcgtt gaaggtgatc tgagtgataa
    11281 tgttagtttg caatcagcga tgaaaggtaa gtatggctta tacagtattc agccaatcgt
    11341 taaagatgat gttagcgaag aattaagaca aggtatgaag ataattgaaa tagctgagca
    11401 agaaaatatt caacatattg tgtatagtac tgcgggaggg gttaatcgaa atcgaactgg
    11461 cccacacttt gaagtactag caaaaataga aaatagatta atggaatcta atataaatgc
    11521 aacagttatc aaaccatcat tctttatgga taatttttta cgcattgcta aagtcgaaga
    11581 tgaacgtatt acattaccag aatttattaa tccgaatata aaattcacaa tgatttcttc
    11641 tattgatata gctaaaattg catcgtatat ttttgcacat ccacaaagct ttacgcatca
    11701 atcaatagag attggttccg atgaagttac attaagtgaa gcagcaacaa tttttagtga
    11761 agtgacaggt aaatctactg ttatagaagg agaatttgtt agtggtgttg cagaaaagca
    11821 atggttggaa gaaaaaggtt atgaagtaga ctttgaatta atggctgaaa taaatccaac
    11881 aagattatca ttaagtgatt ggcttaaagt ccaaaactat aataagtaat gaatggggtg
    11941 caatcatgat tcaatcaatg tggtttaatt tgcatgtgca agatttagaa aagagcgcac
    12001 agttttataa agcgttagga tttaaaataa atagaaaccc acaaatgtta gataaaatgg
    12061 tcggcattca aataggtcaa acaaccgtaa ttttaataga aaacaagcat tttcaaaatg
    12121 taagtcagca aagccttaat actgaaccaa atgaagtgat gatttctcta ggtgtgaaca
    12181 caaatgaaga agttgaccag ttggtgaata aagtgaaaga agcgggtggc acagtcgttc
    12241 aagaaccaac agtaagccaa gggttttatg gagcaatgtt caaagatctt gatggacacc
    12301 attttaattt tttggtctgc taaaatgaaa ttctcaaaag aataaataaa aagcccatac
    12361 agagatgaag cgtaatgtgc accatggtgc gacacattag tgtcttcaat cagtatgggc
    12421 tttgtttatg aggtcagttg gcaccttaag tgcaatacct cgtgagcaaa tattaatttt
    12481 gaatataacc gtattgttgg cgaagatcat aaactttatc tggtgtgaca tcctgaccga
    12541 ttggatcaac cacttttatt gtggaaaatg ttgttaatac ttgtcctcgg atcatgctta
    12601 agtagtcttg acgcaatgca tgttgttctt gtttttcagc tacagtaagt ggttgtactt
    12661 tttctttatt tgcaagttca ttaattctat ctaaaatttt catattgtca tatcctcctt
    12721 agaatcaagt gttgatatat tttgagatta tttttatatg gtaatttatc aacacattaa
    12781 gtaatgtttt tagtttctcg gtcttttgac cagcagattt ttggaattaa aataagggat
    12841 ttgagacacg attttactag tagtacgatg gcaaagtgat gttattgaag tgtcgtttga
    12901 tatacatgtc tgcaatgttg ttttataaga caggatgacg taactgagca aagaaatgag
    12961 cattgtcgaa tgacatcgtg tgtcttatta aaattgcaat gggcatagca taggtatatt
    13021 cacagtcgat tttcttgatt tctacattat cataatacgt agtgtaaaag ttatacgaca
    13081 gtcgatagtc gattcgcttt ttaccacgag caattattat gtgttgcttc aacgtcgact
    13141 ttaatttatt tgtaatgtat gtcactacac ccagactact ctgattaata tttaatatat
    13201 tatgcgtgcg ttcaaacccc tgaggttcat ctgtctcgct cacgtcaatc cctcctatct
    13261 tagtatttaa acaacgcata tcaactatgc aacatatgtt gtttagacat actatactac
    13321 tatgtcgtac gtttgccaat aaacacgcac atatcatttg ttgcctaaac aacatattgc
    13381 ctgaagtgtt gtttaagtac gcaaaaacaa aaatgcttgt cactaaaatc tatatgtttt
    13441 aatgacaagc ttattttcat tcagttaacg actgctaata tgacattgta gagtatagga
    13501 cgttgattta tgtcctagac acggctaatc aatcgtaata cgttaaattt tattgtgtgt
    13561 acaagtatga acctaagtca catagtgatt tcaagatgca tgactaaata ccaagtttta
    13621 ttaaaaatgt gctatatgat gaattaaaaa tggaaggttg cgatcgataa acacatcggc
    13681 tgtctcttga ctcgtgttaa accaatgtaa tgctaagcct gagataccag ctgctaaaaa
    13741 actcgcacta tttacaatga tatctcgttg atgctccgga tagctgtttt ctaacataat
    13801 cgcaaaaata tcttctaatt cattattaat acgttgatgt gctttgttgc aaaatgcttc
    13861 actatttaat tcgcaagact ttgcagcatc tttgatataa ctcgcaattg aaatgaaaat
    13921 attacgcaga accttttcat taatgacatt agaaatgctc aaattatcat tcaagtcttt
    13981 taaaatggtt acagataatg tgtagtctag gaggtcttct ttatcagtga aatgagcgta
    14041 aaatgttgca cgatttagcg tagcaatatc agtgatatct ttaactgtaa tttgagataa
    14101 tttcttttct ctagaaattt taagaaaagc atcgacaagc aattgtttgg ttctaacgat
    14161 tcttggatct atggatttac tttccatcat ttgttcacta cctaacatct gttgttttaa
    14221 cacttattat cagtatcttc tattatattg tcaattgtgt tgatgatatg taagtgctga
    14281 tacgctccta acaaataatg tacctttttg gtaagcattt gaatgtcttt cttctattgt
    14341 attaatggat tttaaaaatg aagataaaat aaaagtgtat tgtaacaata tgtttgaaaa
    14401 attgccaatt tctttatctg aaaattcaga attttgtgat atactatttt tcatagtctt
    14461 gagttataga tgatactgct agcctaaata taacaacatc ggcatgcaca tcttagaatt
    14521 taagctagtg gcagcttata acttaaggct ctttattatt tgcatacaga tgtatataca
    14581 taaatggaag gatgatatta tgacatcaat taaaacaatt acacccaaag attttatatt
    14641 tagagtttta tcaggtgtcg ccataggtat tgttgcgggt ttagttccta atgcgatact
    14701 tggagaaatt tttaaatact ttatggatta tcatccgatt tttaaaacgt tattaggcgt
    14761 tgtcgttgca attcaattta cagtacctgc tttgattggt gcattggttg ccatgaaatt
    14821 tgacctatcg ccattagcaa ttgctgttgt ggcaagtgca gcatatgtag gaagcggtgc
    14881 agcgcagttt aaaaatggtg cttggatgat tacgggtatt ggtgatttaa ttaatacaat
    14941 gattacagca gcgattgcag tgttattcat tttacttatt caacatcgcg taggtagtat
    15001 ggcattgatt gtattcccaa cagttgttgg cggtatttca ggtgcaattg gtgttttaat
    15061 attgccgtat acgaaaatga ttacaacagc catcggtaat atggttaatg gttttacaga
    15121 attacaaccg attgtcatga gtattttgat ttcaatggta tttagtctta ttattatttc
    15181 gccactatca actgtggcca ttgcctttgc gataggtatt acaggtttag cagctggatc
    15241 agcgtcaatt ggtatctccg cgactgaagc agtattgata attggtacaa gtaaagtgaa
    15301 tcgtttaggg gtgccgttat ccgtcttctt tggaggcgtt aaaatgatga tacctaatat
    15361 ggtgaaatat ccaattctta tgttaccaat attaacgaca gccattgtat caggtttggt
    15421 atcagcatta gtaggtattc atggaacgaa agagtcagct ggatttggtt ttataggaat
    15481 ggtaggaccg attaatgcat ttaaatttat ggaggttgat tctgcttggt tgagtgtctt
    15541 actaattgta gtagcgttct ttgttgtacc atttgtaaca gcttggcttg cagatatcat
    15601 ttatcgtaaa gtgtttcgtt tatatactaa tgatattttt aaattcatgg gataataaca
    15661 gtatgttttt tgaaaatatg agaccaaaaa ttatttaatg ttattgtgaa ttgcgagaat
    15721 gaagttgaac aatataattg agctgcagtt tatttgcgct aataaaagat aagacaattc
    15781 acaaaagatg tgtgttttaa aactttgaat atacaacaat aatagcaatc aatacatgtg
    15841 tagaaacata tttgttgatt gctttttaat tgcgtttaat gtacgaaaat aagagaagtt
    15901 acatgtcaga tatattacag ctatgtaaca aaaatacaat ctgtaatatt acgaaagctg
    15961 taatagttga gctaaatata gtaaaaatct tatgtgttaa agactatttc aagggttgat
    16021 tggattctaa agggcacata tttcacatta cagtataaaa cagccatatt tcaacattac
    16081 aaataacact tgatattgta atgttttgta aagaaagtgt aatttactgg ctggtttttt
    16141 gtgatatagt gagtaccgtt gaaagaaata aaagacaatt taaacttgca ctacgacatt
    16201 caaattcaaa atataattac taaaaacata aacttacaat tattactgga ggatttttta
    16261 ctatgaaaaa gacaattatg gcatcatcat tagcagtggc attaggtgta acaggttacg
    16321 cagcaggtac aggacatcaa gcacacgctg ctgaagtaaa cgttgatcaa gcacacttag
    16381 ttgacttagc gcataatcac caagatcaat taaatgcagc tccaatcaaa gatggtgcat
    16441 atgacatcca ctttgtaaaa gatggtttcc aatataactt cacttcaaat ggtactacat
    16501 ggtcatggag ctatgaagca gctaatggtc aaactgctgg tttctcaaac gttgcaggtg
    16561 cagactacac tacttcatac aaccaaggtt caaatgtaca atcagtaagc tacaatgcac
    16621 aatcaagtaa ctcaaacgtt gaagctgttt cagctccaac ttaccataac tacagcactt
    16681 caactacttc aagttcagtg agattaagca atggtaatac tgcaggtgct actggttcat
    16741 cagcagctca aatcatggct caacgtactg gtgtttcagc ttctacatgg gctgcaatca
    16801 tcgctcgtga atcaaatggt caagtaaatg cttacaaccc atcaggtgct tcaggtttat
    16861 tccaaactat gccaggttgg ggtccaacaa acactgttga ccaacaaatc aacgcagctg
    16921 ttaaagcata caaagcacaa ggtttaggtg cttggggatt ctaattcctt cataataaat
    16981 aaaagtaatg tttagacatt aaatatatag ctcttcccat agaaattggg gagagttttt
    17041 ttattaatta aaaatacatt ttttaaaaag tggatattga agttagggaa atttattgga
    17101 ctttattgta ttactgctaa aatacttgtc tttattaagt gccaagtgga atgaaacttc
    17161 atttgaggct gtactgagta cactagatac aaactaaacg cacattttat aattaaatta
    17221 aatgaaaaag gagataaaaa tggctataaa cataattgaa tataataggt cttataagga
    17281 agagctaatc gagtttattt tatcgattca aaaaaatgaa tttaatataa aaattgatag
    17341 agatgatcaa cctgatttgg agaacatcga acataactat ttgaataatg gagggcaatt
    17401 ttggcttgca ataaacaatc atcaaaatat tgttggtact ataggtttga ttagactaga
    17461 taataatatg tctgcactta aaaaaatgtt tgttgataaa ggttatagga atttaaaaat
    17521 aggtaaaaaa cttttagata aagttataat gacatgtaaa gaacaaaata tcgatggtat
    17581 ttatttagga acaattgata aatttattag tgcacagtat ttttattcta ataatggctt
    17641 ccgtgaaatt aagcgaggag atttaccaag tagttttcct aaattagatg tagataatag
    17701 gttttattac agaaatttaa aagattaatt cgatgcaata ataattgata tgacaatctt
    17761 agtaatattc attttaaaag tgacacatag cgtttaaata taaagtctga cgagatatat
    17821 cgtttggttt acattagagg ataggctgca tttcgctttt ttttcaatac atgttaattt
    17881 attcattata taaattttga aaaagtatat tcgaatagca tatcttaatg attctgttac
    17941 agtaatatgc ataaaatata aaacgaacct tttttgtaaa aatataatga aaataacagt
    18001 tattttgcta aaaattcaag aatctatgat aatctatgtt atgtagtaaa atcgacaaat
    18061 ttatatgtgg tttgcagtaa tgaaatcaac tgtaataatt gttttctact taataaaaaa
    18121 tttaaaacta tcttaattaa gggttaaatc aattaatgaa aatcagtgca atattttatt
    18181 ttcaaaaata ttgtgatatg ctacaaaata gagaaaaata aatggggcgt ttattaacat
    18241 ggatacaaaa gactttaaac gtttagaaaa aatgtattcc ccgcgatact tacctggatt
    18301 agatggattg agggcattcg cagttatagg aatcattatt tatcacttga atgcacaatg
    18361 gttaagtggg ggctttttag gagtagatac attcttcgtt atttcaggtt atttaataac
    18421 aagtttgttg ataagtgagt attatcggac gcaaaaaatc gatttgctag agttttggaa
    18481 gcgacgattg aaacgactca ttccggcagt gttattttta atctgtgtcg tgcttacgtt
    18541 cacactgata tttaaaccgg aattgattat acaaatgaaa cgagatgcta ttgcagctat
    18601 attctatgtt tcaaactggt ggtacatttc acagaatgta gattatttta accaatttgc
    18661 tattgaacca ctcaaacatt tatggtcttt agccattgaa gaacaatttt acttgctttt
    18721 cccattggtt atcacgttct tattacatag atttaaaccg agaaatatta ttcaaacgct
    18781 atttattgta tcgttgattt ctttaggact tatgatagtg attcatttca tcactggaga
    18841 taattcacgt gtgtattttg ggacagatac acgactgcaa actttattgc ttggttgtat
    18901 attagcattt atttggcctc cgtttgcttt gaaaaaagat atttctaaaa agattgtcgt
    18961 atcattagat attataggga tatctggttt tgcggttcta atgactttgt tctttatagt
    19021 tggagaccaa gatcaatgga tctataatgg aggattttac attatatcat ttgcaacttt
    19081 attcattatt gcaattgcgg tacatccttc tagtttattt gctaaatttt taagtatgaa
    19141 acctttacta attataggta aacgatcata tagcttatac ttatggcatt atcctatcat
    19201 tgtttttgtg aacagttatt acgtacaagg acaaataccg gtatacgttt atattataga
    19261 aattttgtta acagcgttaa tggctgaaat ttcgtatcgc tttattgaaa cacctatacg
    19321 taaaaaagga tttaaagctt ttgcattttt acctaaaaag aaggggcaat ttgctagaac
    19381 agtgttagtt atcctattat tggttccgtc tatcgttgtg ctcagtggac agtttgatgc
    19441 acttggcaaa caacatgaag ccgagaagaa agagaagaag acggaattta aaacaacgaa
    19501 gaaaaaagtc gttaaaaaag ataagcaaga ggataagcag acagcgaata gcaaagagga
    19561 tattaaaaag tcatcaccac tattaattgg tgactcggtc atggtggata ttggtaatgt
    19621 ctttactaag aaaataccaa atgcacaaat tgatggtaaa gttggacggc aactcgttga
    19681 tgctacacca attgtgaaat cgcaatataa agactatgct aaaaaaggtc aaaaagttgt
    19741 agtagagctt ggtacaaatg gggcatttac gaaagatcaa ttaaatgaac tattggatag
    19801 ttttggaaaa gcagacatat atttagtttc tattagagta cctagagatt atgaaggtag
    19861 aataaataaa ttaatttatg aggcagctga aaagcgctct aatgtacatc tagtcgattg
    19921 gtataaagct tctgcaggtc atccggaata ctttgcatat gacggtattc acttagaata
    19981 tgcaggtagt aaagcgctga ctgatttgat tgtaaaaacg atggaaacac atgctacaaa
    20041 taagaaataa tttgatgcac taaacttttg aaatattaca ttacttctga tatttattat
    20101 caaaaatgat gtatttcatt aaaagtttag tgctttttta ttttcaaatc ccatagtaac
    20161 ggtgcagaaa aagtgttgta aacattctaa ttggtatatt acattcaatg aagctttatt
    20221 aggaacagat tacattatga taacaaagcc cgcaagacac ctaatctctg ttatagtttg
    20281 ttttgtcgca aaactataaa agttataatt gtttgcatac taaaaaaata aaaaatataa
    20341 aatttaaaat aattgagtcg ctaatgacta tatgtataga gtgttttgat tattgggagg
    20401 atatttaatt atgaaaaaaa tcgttacagc tacaatcgct acagcaggac ttgccactat
    20461 cgcatttgca ggacatgatg cacaagccgc agaacaaaat aacaatggat ataattctaa
    20521 tgacgctcaa tcatacagct atacgtatac aattgatgca caaggtaatt atcattacac
    20581 ttggacagga aattggaatc caagtcaatt aacgcaaaac aacacatact actacaacaa
    20641 ctacaatact tatagttata acaatgcatc ttacaataac tactataatc attcatatca
    20701 atacaataac tatacaaaca atagtcaaac agcaacaaat aactattata ctggtggttc
    20761 aggtgcaagt tatagcacaa caagtaataa tgttcatgtg actacaactg cagcgccatc
    20821 ttcaaatggt cgttcaattt ctaatggtta tgcatcagga agtaacttat atacttcagg
    20881 acaatgtact tattatgtat ttgatcgtgt tggtgggaaa attggttcaa catggggtaa
    20941 cgcaagtaat tgggctaacg cagctgcatc atctggctat acagtgaaca atacaccaaa
    21001 agttggtgct atcatgcaaa caacacaagg ctattacggt catgttgctt acgttgaagg
    21061 cgttaacagc aacggttctg ttcgtgtttc agaaatgaac tatggacatg gtgctggtgt
    21121 ggttacgtct cgtacaattt cagcaaacca agcaggttca tataatttca ttcattaatc
    21181 aaatgtaaat caaatgacgt caatattctc taacatgaga gtattggcgt ttttgtttta
    21241 tataaatata aatgagagcg gtttattcac tgatctttag ggaactaagt aataaagtga
    21301 taatttatac tatgtcagta tgattgagag tgattcaatt tagatgaaaa ccatgaaaaa
    21361 atatattaaa acagcatttt tttgtagtat gtattggtta attgttcaac taaatatagc
    21421 aaatttaggt acaagaattc ctgataagta ttttcgtcag aagtacataa tatttaaatc
    21481 atttaacttt gagaagcatg gaaaattttg gaacaaatgg ttttacgtaa gaaaatggaa
    21541 acataagatt ttagatggtc atcagcttaa tcaaaatata tatgatcagc gtcatttaat
    21601 gacaatcaat actgatgaaa ttgaaaaaat gattatagag acaaagaggg cagagttgat
    21661 tcattggata tcgatacttc cagtcatcat attcaataaa ggccctcgtt tagtaaagta
    21721 tataaatatt ttctatgcaa tgatagctaa tgttccaatc attattgtgc aacgctataa
    21781 tcgaccgaga ttaacgcagt tactacgcat attaaaacga agaggtgaac gtcatgacta
    21841 aacatatcat cgttattggt ggtggcttag gtgggatttc tgcagcaatt cgaatggcac
    21901 aaagtggcta ttcggtctca ttatatgaac aaaataatca tataggaggc aaagtgaatc
    21961 gtcatgaatc agatggcttt ggctttgatt taggtccatc tattttaacg atgccttata
    22021 tttttgaaaa attattcgaa tatagcaaga agcaaatgtc agactacgtt acaatcaagc
    22081 gattgccaca tcaatggcgt agcttttttc cagatggaac gactatcgat ttgtatgaag
    22141 gtattaaaga aacaggtcag cataatgcga tattgtcgaa acaggatata gaggaactgc
    22201 aaaattattt gaattataca agacgaatcg atcgtattac tgaaaaaggg tatttcaact
    22261 atggtttaga tacactatct caaattatta aatttcatgg gccattaaat gctcttatta
    22321 attatgatta tgtacatact atgcaacagg ccatagacaa gcgtatctcg aatccatact
    22381 tgcgacaaat gttaggctat tttatcaaat atgtaggttc ttcatcatac gatgcgccag
    22441 ctgtattatc tatgttattc catatgcaac aagagcaagg cctttggtat gtagaaggtg
    22501 gaatccatca tttagccaat gccttggaaa agctagcgcg tgaagaaggt gtcacaattc
    22561 atacaggtgc acgtgtggac aatattaaaa catatcaaag acgtgtgacg ggtgtcagat
    22621 tagatacagg tgagtttgta aaggcagatt atattatttc aaatatggaa gtcataccta
    22681 cttataaata tttaattcac cttgatactc aacgattaaa caaattagag agggaatttg
    22741 agccggcaag ctcaggatat gtgatgcatt taggtgttgc ttgccaatac ccgcaattag
    22801 cacatcataa tttctttttt acggaaaatg cttatctcaa ttatcaacaa gtttttcatg
    22861 aaaaggtatt gccagatgat ccgaccattt atctagtaaa tacgaataaa actgatcaca
    22921 cacaagcgcc agtaggttat gaaaatatca aagtcttacc acatattcca tatattcaag
    22981 atcagccttt taccactgaa gattatgcga agtttaggga taaaattttg gataaattag
    23041 aaaaaatggg acttactgat ttaagaaaac acattattta tgaagatgtt tggacaccgg
    23101 aggatattga aaaaaattat cgttctaatc gtggtgcaat atatggtgtt gtagcagata
    23161 aaaagaaaaa caaaggattt aaatttccta aagaaagtca gtattttgaa aacttgtact
    23221 ttgtaggtgg atcagtaaat cctggtggtg gcatgccaat ggttacatta agtgggcaac
    23281 aagtcgcaga caaaataaac gcgcgagaag cgaagaatag gaagtgatat ctatgaaatg
    23341 gttatcacga atattaacag taatagtgac catgtctatg gcgtgtggtg cattgatatt
    23401 taatcgtaga catcagctaa aggcgaaaac gctgaacttc aatcataaag cattaacaat
    23461 tattattccg gctagaaacg aagaaaaaag aataggtcat ttactacatt cgataataca
    23521 acagcaagtt ccagtagatg tcattgttat gaatgacgga tcgacagatg aaacagcacg
    23581 tgtagcacgt tcatatggtg cgactgtagt agatgttgtt gatgatactg acggcaaatg
    23641 gtatgggaaa tcacatgctt gttatcaagg tgtgacgcat gcatgtacga atcgcattgc
    23701 ctttgtagat gctgatgtaa ctttcttaag gaaagatgct gttgaaacgt tgattaatca
    23761 gtatcaatta caaggtgaaa aaggattgtt aagcgtacag ccttatcata taacaaagcg
    23821 tttctacgaa gggttttcag cgatatttaa tttaatgaca gtcgttggta tgaatgtatt
    23881 ttctacctta gacgacggtc ggactaacca gcatgcattt ggaccggtga cattaacaaa
    23941 taaagaagat tattatgcaa ctggaggtca taaaagtgca aaccgtcata ttattgaagg
    24001 atttgcttta ggaagtgcat atacttcaca atcattgccc gtaacagttt atgaagggtt
    24061 tccatttgtt gcatttcgca tgtatcaaga aggatttcag tcattacaag aaggatggac
    24121 aaagcatttg tcaactgggg caggtggcac aaagcctaag atcatgacag caattgtgtt
    24181 gtggttgttt ggttctatag cgagtatttt agggctatgt cttagtttaa aatatcgcca
    24241 aatgtctgta agaaaaatgg tagcacttta cttgagctat actacacaat ttatttatct
    24301 gcatcgaagg gtcggccaat tttctaattt attaatggta tgtcatccat tgttatttat
    24361 gttttttact aaaattttca tccaatcttg gaaacaaacg catcgttatg gtgtagttga
    24421 atggaaaggt cgtcaatatt ctatatctaa agaacaataa atcaaggtaa tggcatttca
    24481 atataggagg actagtatga caatgatgga tatgaatttt aaatattgtc ataaaatcat
    24541 gaagaaacat tcaaaaagct tttcttacgc ttttgacttg ttaccagaag atcaaagaaa
    24601 agcggtttgg gcaatttatg ctgtgtgtcg taaaattgat gacagtatag atgtttatgg
    24661 cgatattcaa tttttaaatc aaataaaaga agatatacaa tctattgaaa aatacccata
    24721 tgaacatcat cactttcaaa gtgatcgtag aatcatgatg gcgcttcagc atgttgcaca
    24781 acataaaaat atcgcctttc aatcttttta taatctcatt gatactgtat ataaagatca
    24841 acattttaca atgtttgaaa cggacgctga attattcgga tattgttatg gtgttgctgg
    24901 tacagtaggt gaagtattga cgccgatttt aagtgatcat gaaacacatc agacatacga
    24961 tgtcgcaaga agacttggtg aatcgttgca attgattaat atattaagag atgtcggtga
    25021 agattttgac aatgaacgga tatattttag taagcaacga ttaaagcaat atgaagttga
    25081 tattgctgaa gtgtaccaaa atggtgttaa taatcattat attgacttat gggaatatta
    25141 tgcagctatc gcagaaaaag attttcaaga tgttatggat caaatcaaag tatttagtat
    25201 tgaagcacaa ccaatcatag aattagcagc acgtatatat attgaaatac tggacgaagt
    25261 gagacaggct aactatacat tacatgaacg tgtttttgtg gataagagga aaaaggcaaa
    25321 gttgtttcat gaaataaata gtaaatatca tagaatatag gtggttgaat aatgaagatt
    25381 gcagtaattg gtgcaggtgt cacaggatta gcagcggcag cccgtattgc ttctcaaggt
    25441 catgaagtga cgatatttga aaaaaataat aatgtaggcg ggcgtatgaa tcaattaaag
    25501 aaagacggct ttacatttga tatgggtccc acaattgtca tgatgccaga tgtttataaa
    25561 gatgttttta cagcgtgtgg taaaaattat gaagattata ttgaattgag acaattacgt
    25621 tatatttacg atgtgtattt tgaccacgat gatcgtataa cggtgcctac agatttagct
    25681 gaattacagc aaatgctaga aagtatagaa cctggttcaa cgcatggttt tatgtccttt
    25741 ttaacggatg tttataaaaa atatgaaatt gcacgtcgct atttcttaga aagaacgtat
    25801 cgcaaaccga gtgactttta taatatgacg tcacttgtgc aaggtgctaa gttaaaaacg
    25861 ttaaatcatg cagatcagct aattgaacat tatattgata acgaaaagat acaaaagctt
    25921 ttagcgtttc aaacgttata cataggaatt gatccaaaac gaggcccgtc actatattca
    25981 attattccta tgattgaaat gatgtttggt gtgcatttta ttaaaggcgg tatgtatggc
    26041 atggctcaag ggctagcgca attaaataaa gacttaggcg ttaatattga actaaatgct
    26101 gaaattgagc aaattattat tgatcctaaa ttcaaacggg ccgatgcgat aaaagtgaat
    26161 ggtgacataa gaaaatttga taaaatttta tgtacggctg atttccctag tgttgcggaa
    26221 tcattaatgc cagattttgc acctattaaa aagtatccac cacataaaat tgcagactta
    26281 gattactctt gttcagcatt tttaatgtat atcggtatag atattgatgt gacagatcaa
    26341 gtgagacttc ataatgttat tttttcagat gactttagag gcaatattga agaaatattt
    26401 gagggacgtt tatcatatga tccttctatt tatgtgtatg taccagcggt cgctgataaa
    26461 tcacttgcgc cagaaggcaa aactggtatt tatgtgctaa tgccgacgcc ggaacttaaa
    26521 acaggtagcg gaatcgattg gtcagatgaa gctttgacgc aacaaataaa ggaaattatt
    26581 tatcgtaaat tagcaacgat tgaagtattt gaagatataa aatcgcatat tgtttcagaa
    26641 acaatcttta cgccaaatga ttttgagcaa acgtatcatg cgaaatttgg ttcggcattc
    26701 ggtttaatgc caactttagc gcaaagtaat tattatcgtc cacaaaatgt atcgcgagat
    26761 tataaagatt tatattttgc aggtgcaagt acgcatccag gtgcaggcgt tcctattgtc
    26821 ttaacgagtg cgaaaataac tgtagatgaa atgattaaag atattgagcg gggcgtataa
    26881 gggagtagtc taagagaaag atgtgagaaa agtataaggg gaacgtaaaa ggcatagtga
    26941 aataagtatg tgcttaagaa gttttattta acaatgaatg acgcctgtta aggttcacgt
    27001 ttgaatacgt accttgatgg gcgttatact ttatataagc atatctaact ggttgcgcat
    27061 gcggatatgc atgacagtta aatcataacg attgagtgca tattaatctg aaaattccta
    27121 atattgacaa taatattata cctacctaaa atgaaaataa attcattcaa tacaacgtgt
    27181 gtcataagtt ctgttgaaca taacggtgaa tggcgatgtc attgtaacca tctaaatgag
    27241 agtgatacaa gttgttagga ttatgatagt cgcccaggct atgtttaatg atagaggagg
    27301 aatttgatga tctctaacaa attagcaaat atccctgata gttatttcgg gaaaacgatg
    27361 ggacgtaaaa tcgaacatgg accattgcca ttaattaata tggcagttgg aatccctgat
    27421 gggcccacgc cacaaggtat tattgatcat tttcaaaaag cactaactat tcctgaaaat
    27481 caaaaatatg gtgcttttca tggtaaagaa gcgttcaagc aagctattgt agatttttat
    27541 caaagacaat ataatgtgac gttagataaa gaagatgaag tatgcatttt atatggtaca
    27601 aaaaatggat tagtggcagt accaacatgt gttatcaatc caggagacta tgtattacta
    27661 cctgatccag gctacacgga ttatttagca ggtgtacttt tagctgatgg caagccagtc
    27721 ccgcttaatt tagaaccgcc acattatttg ccagattggt ccaaagttga ttcacaaata
    27781 atagataaaa caaaactcat ttatttaacg tatccaaata atccaactgg atcgacagct
    27841 acgaaagaag tttttgatga agcgatagct aaatttaaag gtacagacac taaaatcgtg
    27901 catgattttg cttatggtgc tttcggtttc gacgctaaaa atcctagtat acttgcttcg
    27961 gaaaatggga aagatgttgc gattgaaatt tattccttgt ctaaaggtta taacatgtca
    28021 ggttttagag tcggttttgc agttggtaat aaagatatga ttcaagcttt gaaaaagtac
    28081 caaacgcaca caaatgctgg gatgtttggt gcattacaag atgccgcaat atatgctttg
    28141 aatcattatg atgatttttt agaagaacaa agcaatgtat ttaaaacacg tcgtgaccga
    28201 ttcgaagcca tgcttgcaaa agctgatttg ccatttgttc atgctaaagg tggcatatat
    28261 gtatggttgg aaacaccgcc tggttatgac agcgagcaat ttgagcaatt tttagtgcaa
    28321 gagaagtcaa tacttgttgc tccagggaaa ccatttggag agaatggtaa tcgttatgtg
    28381 agaatttcat tggcgttaga tgaccagaaa ttagacgaag ctgcaataag attaacagaa
    28441 ctagcatatt tatatgaata ataaagttaa gggtgatgtt aaaatgacaa aaattaaaat
    28501 catgagtgta cgtgatgaag atatgcctta tatcaaggcg tgggcagaga aacatcatgt
    28561 tgaggtagat attactaaag aagcattaac tgatgacaat gttgaaggtg tcgctggtta
    28621 tgatggctta tcgctatcac aacaaatacc attatcagag catgtttata aacgattaaa
    28681 tgaactaggt attaaacaaa tcgcacaacg tagtgctgga tttgatacat atgatttgga
    28741 gttagcaaac aagtataact ttattgtgtc aaatgtgccg tcatactctc cgaattcaat
    28801 tgcggagttt gctgtgaacc aggctattaa tgtggttcgt cattttaatc aaattcaaac
    28861 gaaagtgcgg gagcatgatt ttagatggga acctactatt ttatcgaaat ccattaaaga
    28921 tttaaaagta gctgttattg gtacaggtcg aattggccgt gtagtagccg atatatttgc
    28981 caatggttat caaagtgatg tggtcgcata cgacccgttt cctaatgcta aaattgcaac
    29041 gtatgtcgat tacaaagata cgattgagga agcggttgaa ggtgctgata tcgtgacatt
    29101 acatgtacct gcaacgaaat ataatcatta tctatttaat gctgaattat ttaaacattt
    29161 taaaaagggc gcagtatttg tcaattgtgc gagaggttct ttagtagata ctaaggcgtt
    29221 attagacgca ttagacaatg gtgtgattaa aggtgcagca cttgatacgt atgaatttga
    29281 acgcaaactt ttcccaagtg atcaaagggg caaaacactg aacgatccat tgttagaatc
    29341 gttgattgac agggaagatg tcatattaac accacatatt gcgttttata ctgaagctgc
    29401 agttaaaaat ctaattgtcg atgcattaga tgcaacatta gatgtattgc agactggaga
    29461 tactaggtta cgagtaaatt aaaaatcgaa ctgatgagat aatttggatt gttgggattc
    29521 tcatcagttc gattttttaa tttggtgttg atgacgttga atattgccta attaaacgac
    29581 atcgtaacct tgatcttcaa ttgcgtcttt catttgagat acagcaactt tactgtcatc
    29641 atattgaaca cttacttgac cattttcaag gttaacgtca gctgaagtga caccgtcaat
    29701 attatttaat gcagattcaa cagcactttt gcagtgacca cagctcatac cttcaacatt
    29761 taaaatttct tgtgacatag cattcacctc cttttagagc cacttattat t
//
LOCUS       DALAGD010000026        28892 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.026, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000026 DALAGD010000000
VERSION     DALAGD010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 28892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28892
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.026"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(109..699)
                     /locus_tag="O2701_000919"
     CDS             complement(109..699)
                     /locus_tag="O2701_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCZ4292824.1"
                     /translation="MKVNPNNIELIISAVKEEQYPETELSEVALSGRSNVGKSTFINS
                     MIGRKNMARTSQQPGKTQTLNFYNIDEQLIFVDVPGYGYAKVSKTQREKFGKMIEEYI
                     TKRENLQLVIQLVDLRHDPTQDDILMYNYLKHFDIPTLVICTKEDKIPKGKVQKHIKN
                     IKTQLDMDPDDTIVSYSSIQNNKQQQIWNLIEPYIS"
     gene            complement(853..2115)
                     /gene="clpX"
                     /locus_tag="O2701_000920"
     CDS             complement(853..2115)
                     /gene="clpX"
                     /locus_tag="O2701_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HCZ4292825.1"
                     /translation="MFKFNEDEENLKCSFCGKDQDQVKKLVAGSGVYICNECIELCSE
                     IVEEELAQNTSEAMTELPTPKEIMDHLNEYVIGQEKAKKSLAVAVYNHYKRIQQLGPK
                     EDDVELQKSNIALIGPTGSGKTLLAQTLAKTLNVPFAIADATSLTEAGYVGDDVENIL
                     LRLIQAADFDIDKAEKGIIYVDEIDKIARKSENTSITRDVSGEGVQQALLKILEGTTA
                     SVPPQGGRKHPNQEMIQIDTTNILFILGGAFDGIEEVIKRRLGEKVIGFSSNEADKYD
                     EQALLAQIRPEDLQAYGLIPEFIGRVPIVANLETLDVTALKNILTQPKNALVKQYTKM
                     LELDDVDLEFTEEALSAISEKAIERKTGARGLRSIIEESLIDIMFDVPSNENVTKVVI
                     TAQTINEETEPELYDAEGNLINNSKTSA"
     gene            complement(2266..3567)
                     /locus_tag="O2701_000921"
     CDS             complement(2266..3567)
                     /locus_tag="O2701_000921"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCZ4292826.1"
                     /translation="MTATWEKKEGNEGLLTVTVPAEKVNKALDQAFKKVVKQINVPGF
                     RKGKVPRPIFEQRFGVEALYQDAIDILLPDAYGEAIDETDIKPVAQPEVSVTQIEKGK
                     DFIFEATVTVEPEVKLGDYKGLEIEKQETELSDDELQEAIDHSLGHLAEMVVKEDGVV
                     ENGDTVNIDFSGSVDGEEFEGGQAEGYDLEIGSGSFIPGFEEQLEGMKVDEEKDVVVT
                     FPEEYHAEELAGKEATFKTKVNEIKFKEVPELTDEIANELDAEANTVDEYKENLRKRL
                     AEQKATDAENVEKEEAITKATDNTTIDIPEAMVNTELDRMVSEFAQRIQQQGLDLQTY
                     FQISGQDETQLREQMKDDAEQRVKTNLTLTAIAEAEKIEATDEDIDKELEKMSKQFNI
                     SVEDIKNTLGNTDIIKNDVRIQKVIDLLRDNAKFVEGTKED"
     gene            complement(3615..3725)
                     /locus_tag="O2701_000922"
     CDS             complement(3615..3725)
                     /locus_tag="O2701_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292827.1"
                     /translation="MQNNTQHFNIVLTKKVDLQIYKRCDFNVCIGNRPMI"
     gene            complement(3730..4659)
                     /locus_tag="O2701_000923"
     CDS             complement(3730..4659)
                     /locus_tag="O2701_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292828.1"
                     /translation="MSDIIQFPNSSKKLYKDIKRAEQDQNYDLMYEYIVQYERQFELT
                     EEIAMMKCRMLYETGSFLELREETIVLLKTGIQQYDALMIYYVKSLIGLGQYFEAVEV
                     IHQIIDEVKDHKTRMALHPLKEFAKSKLIEDEKRLTQSLADFDTLSMREQTHLILKLI
                     DNGHFQFQETVLYILKSNTYSYNLISLMIEYLRFANCTQELTIEKYGMDVTFVPANLK
                     GLEHTTLKEKVIPNVMETLNDGALHIAEEAHHIMNNHSIMIYPIDIETLFETNKWINA
                     YECYFKNMLGLKCELQSIDAFNFIQQLDLNNNS"
     gene            complement(4678..5286)
                     /locus_tag="O2701_000924"
     CDS             complement(4678..5286)
                     /locus_tag="O2701_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCZ4292829.1"
                     /translation="MSKFDEQIIVVPREIIFNNEKNTFNGFLNKNKPEGQNIFDALSQ
                     YEVKRRGDMEEDPSYKQLISYCLLENEHGEILVYERLSGGGEARLHGQSSIGVGGHMN
                     DVPGAESINEVLRVNAQRELEEEVGLSEQDSQNMEYIGFINDDNNEVGKVHIGVVFKI
                     TVSTNDVEAKETDTLRIKWVEKGNIESYDDFETWSALILQDL"
     gene            complement(5428..5784)
                     /gene="rplT"
                     /locus_tag="O2701_000925"
     CDS             complement(5428..5784)
                     /gene="rplT"
                     /locus_tag="O2701_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCZ4292830.1"
                     /translation="MPRVKGGTVTRARRKKTIKLAKGYFGSKHTLYKVAKQQVMKSGQ
                     YAFRDRRQRKRDFRKLWITRINAAARQHEMSYSRLMNGLKKAGIDINRKMLSEIAISD
                     EKAFAQLVTKAKDALK"
     gene            complement(5831..6031)
                     /gene="rpmI"
                     /locus_tag="O2701_000926"
     CDS             complement(5831..6031)
                     /gene="rpmI"
                     /locus_tag="O2701_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCZ4292831.1"
                     /translation="MPKMKTHRGAAKRVKRTASGQLKRSRAFTSHLFANKSTKQKRQL
                     RKARLVSKSDMKRVKQLLAYKK"
     gene            complement(6060..6587)
                     /gene="infC"
                     /locus_tag="O2701_000927"
     CDS             complement(6060..6587)
                     /gene="infC"
                     /locus_tag="O2701_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCZ4292832.1"
                     /translation="MSTIAKDQTQINDKIRAKELRLIGQDGEQIGVKSKREALEMAER
                     VDLDLVVVAPNAKPPVARIMDYGKFKFEQQKKEKEMKKKQKIINVKEIRLSPTIEEHD
                     FQTKLKNGRKFLTKGDKCKVSIRFRGRAITHKEIGQRVLEKYADECKDIATVEQKPKM
                     DGRQMFIMLAPTAEK"
     misc_feature    complement(6616..6741)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            complement(6816..8309)
                     /locus_tag="O2701_000928"
     CDS             complement(6816..8309)
                     /locus_tag="O2701_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCZ4292833.1"
                     /translation="MSKVQNESNNVVKRGLKDRHISMIAIGGCIGTGLFVTSGGAIHD
                     AGALGALIGYAIIGIMVFFLMTSLGEMATYLPVSGSFSTYATRFVDPSLGFALGWNYW
                     FNWVVTVAADITIAAQVIQYWTPLQGIPAWAWSALFLVIIFSLNSLSVRVYGESEYWL
                     ALIKVVTVIVFIAIGLLTIVGIMGGHVVGFEIFNKGEGPILGGNLGGSLLSILGVFLI
                     AGFSFQGTELIGITAGESENPERAVPKAIKQVFWRILLFYILAIFVIGMLIPYDSSAL
                     MGGSDNVATSPFTLVFKNAGFAFAASFMNAVILTSVLSAGNSGMYASTRMLYSMSKDK
                     LAFEAFGKTNKNGVPYMSLLVTAIIVVIIFVLQSLTKGAYEYIVAASGLTGFIAWVGI
                     AVSHYRFRRAFDKQNYDKSKLKYTAKLFPFGPLFAGFLCIVVIIGQDVDFIKTGDFNF
                     SRFLITYMGIPVFLAFFIYHKLRYKTKKIPLEQVDLRQDVSMDEIKG"
     gene            complement(8735..10672)
                     /gene="thrS"
                     /locus_tag="O2701_000929"
     CDS             complement(8735..10672)
                     /gene="thrS"
                     /locus_tag="O2701_000929"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCZ4292834.1"
                     /translation="MEQINIQFPDGNKKAFDKGTTTEDIAQSISPGLRKKAVAGKFNG
                     QLVDLTKPLETDGSIEIVTPGSEEALEVLRHSTAHLMAHAIKRLYGNVKFGVGPVIEG
                     GFYYDFDIDQNISSDDFEQIEKTMKQIVNENMKIERKVVSRDEAKELFSNDEYKLELI
                     DAIPEDENVTLYSQGDFTDLCRGVHVPSTAKIKEFKLLSTAGAYWRGDSNNKMLQRIY
                     GTAFFDKKELKAHLQMLEERKERDHRKIGKELELFTNSQLVGAGLPLWLPNGATIRRE
                     IERYIVDKEVSMGYDHVYTPVLANVDLYKTSGHWDHYQEDMFPPMQLDETESMVLRPM
                     NCPHHMMIYANKPHSYRELPIRIAELGTMHRYEASGAVSGLQRVRGMTLNDSHIFVRP
                     DQIKEEFKRVVNMIIDVYKDFGFEDYSFRLSYRDPEDKEKYFDDDDMWNKAENMLKEA
                     ADELGLSYEEAIGEAAFYGPKLDVQVKTAMGKEETLSTAQLDFLLPERFDLTYIGQDG
                     EHHRPVVIHRGVVSTMERFVAFLTEETKGAFPTWLAPKQVQIIPVNVDLHYDYARQLQ
                     DELKSQGVRVSIDDRNEKMGYKIREAQMQKIPYQIVVGDKEVENNQVNVRQYGSQDQE
                     TVEKDEFIWNLVDEIRLKKHR"
     gene            complement(10870..10992)
                     /locus_tag="O2701_000930"
     CDS             complement(10870..10992)
                     /locus_tag="O2701_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292835.1"
                     /translation="MFDENMVNKLDDYYFIYGICNEYPDQDRYLKQRYLIHKLY"
     gene            complement(11085..12005)
                     /gene="dnaI"
                     /locus_tag="O2701_000931"
     CDS             complement(11085..12005)
                     /gene="dnaI"
                     /locus_tag="O2701_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HCZ4292836.1"
                     /translation="MKQFKSIINTSQDFEKRIEKIKKEVINDPDVKQFLEAHRAELTN
                     AMIDEDLNVLQEYKDQQKHYDGHKFADCPNFVKGHVPELYVDNNRIKIRYLQCPCKIK
                     YDEERFEAELITSHHMQRDTLNAKLKDIYMNHRDRLDVAMAADDICTAITNGEQVKGL
                     YLYGPFGTGKSFILGAIANQLKSKKVRSTIIYLPEFIRTLKGGFKDGSFEKKLHRVRE
                     ANILMLDDIGAEEVTPWVRDEVIGPLLHYRMVHELPTFFSSNFDYSELEHHLAMTRDG
                     EEKTKAARIIERVKSLSTPYFLSGENFRNN"
     gene            complement(12005..13405)
                     /locus_tag="O2701_000932"
     CDS             complement(12005..13405)
                     /locus_tag="O2701_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HCZ4292837.1"
                     /translation="MGRQAFEFGLRPKDQFKVMQHFDLNTNHLEVLNRLYTPLIGTQA
                     VGLYHFMTQFVKESHNETLILSHYIFMNELKINLLEFRQQMDLLEAIGLLKVFVKHDE
                     QETQFVYQLIQPPSAHLFFNDPMLSIFLYSEVEHRRFHELKKYFEYQQIDLSEFKQVT
                     RQFTDVFKVPSTKIDIDTSDIPINEPYQGIDLSNESFDFEMLRQMLGKHFISQDIVTK
                     DAKRLITQLATLYGLTADGMKHVILNSITSGQQLSFEEMRKQARSYYLMEHENQMPKL
                     QVKSPATSSSAGKSSEVNPKPQSDEWFELLEQTSPIDMLASWSESEPTISQKTMVEEL
                     IEREKMSFGVINILLQFVMLKEDMKLPKAYILEIASNWKKKGIKTAKEAYNYAKKVNQ
                     PKNEGSSGNYQKRGSYYGQRNRISKEKTPKWLENRDKPSEEDSAKDNSVDDQQLEQDR
                     QAFLDKLSKKWEEDSQ"
     gene            complement(13406..13876)
                     /gene="nrdR"
                     /locus_tag="O2701_000933"
     CDS             complement(13406..13876)
                     /gene="nrdR"
                     /locus_tag="O2701_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HCZ4292838.1"
                     /translation="MKCPKCNSTQSKVVDSRHADELNAIRRRRECENCGTRFTTFEHI
                     EVSQLIVVKKDGTREQFSREKILNGLVRSCEKRPVRYQQLEDITNKVEWQLRDEGHTE
                     VSSRDIGEHVMNLLMHVDQVSYVRFASVYKEFKDVDQLLASMQGILSENKRSDA"
     gene            complement(14086..15111)
                     /gene="gap"
                     /locus_tag="O2701_000934"
     CDS             complement(14086..15111)
                     /gene="gap"
                     /locus_tag="O2701_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCZ4292839.1"
                     /translation="MSTNIAINGMGRIGRMVLRIALQNKNLNVVAINASYPPETIAHL
                     INYDTTHGKYNLKVEPIENGLQVGDHKIKLVADRNPENLPWKELDIDIAIDATGKFNH
                     GDKAIAHIKAGAKKVLLTGPSKGGHVQMVVKGVNDNQLDIEAFDIFSNASCTTNCIGP
                     VAKVLNNQFGIVNGLMTTVHAITNDQKNIDNPHKDLRRARSCNESIIPTSTGAAKALK
                     EVLPELEGKLHGMALRVPTKNVSLVDLVVDLEKEVTAEEVNQAFENAGLEGIIEVEHQ
                     PLVSVDFNTNPNSAIIDAKSTMVMSGNKVKVIAWYDNEWGYSNRVVDVAEQIGALLTS
                     KETVSAS"
     gene            complement(15281..15904)
                     /locus_tag="O2701_000935"
     CDS             complement(15281..15904)
                     /locus_tag="O2701_000935"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCZ4292840.1"
                     /translation="MPKVIGLTGGIASGKSTVSELLSVFGFKVVDADKAAREAVKKGS
                     KGLAQVREVFGDEAIDENGEMNRRYMGDLVFNHPEKRLELNAIIHPIVRDIMEEEKQE
                     YLKQGYNVIMDIPLLFENELENTVDEVWVVYTSESIQMDRLMQRNNLSLEDAKARVYS
                     QISIDKKSRMADHVIDNLGDKLELKQNLERLLEEEGYIEKPNYGEED"
     gene            complement(15920..16792)
                     /gene="mutM"
                     /locus_tag="O2701_000936"
     CDS             complement(15920..16792)
                     /gene="mutM"
                     /locus_tag="O2701_000936"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCZ4292841.1"
                     /translation="MPELPEVEHVKRGIEPYVINQKIEHVIFSDKVIEGKAQGKETII
                     KGIELDTFKTLSEGYTITNVERRSKYIVFQLDNKREQRTLISHLGMAGGFFIVDELED
                     IMIPNYRKHWHVIFELSNDKKLIYSDIRRFGEIRNVASVASYPSFLEIAPEPFSNEAL
                     TYYLNRIHQQSNKNKPIKQVILDHKVIAGCGNIYACEALFRAGVLPDKKVKDLTHQQQ
                     EMVFYYVREVLEEGIKYGGTSISDYRHADGKTGEMQLHLNVYKQPVCKVCGSQIETKI
                     IATRNSHYCPVCQK"
     gene            complement(16808..19438)
                     /gene="polA"
                     /locus_tag="O2701_000937"
     CDS             complement(16808..19438)
                     /gene="polA"
                     /locus_tag="O2701_000937"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCZ4292842.1"
                     /translation="MNKLVLIDGNSLSFRAFYALPLLSNKAGIHTNAVYGFAMLLEKI
                     LKEEKPNHFLVAFDAGKTTFRHEKYSEYKGGRQKTPPELSEQFPYIRQLLDAYHIKRY
                     ELDNYEADDIIGTLSKEADKAGFQTIIITGDRDLTQLATDNVTIYYTKKGVTDVDHYT
                     PDFIAEKYNGLTPNQIIDMKGLMGDTSDNIPGVAGVGEKTAIKLLNQFDTVEGVYEHL
                     DEISGKKLKEKLQNSKEDALMSKELATINVDSPIEVKLEDTLMTHQDEQQEKIELFKK
                     LEFKQLLADIDQSASVEDAIEKTFEIETSFDNIDFTSLKEAAIHFELDGGNYLRNNIL
                     KFSLFTGEKHIVINADDINNYVELVSWLENPNSKKVVYDAKKTYVASHRLGIDIQNIS
                     FDIMLASYIIDPSRTISDVQSVVSLYGQSFVKDDVSIYGKGKKFKVPEDDVLNPYVAS
                     ITDAIYFAKPNMDKQLEEYNQVELLADLELPLAKILSEMEEIGIFTDVHDLEEMEKEI
                     QEKLDVLIRNIHDAAGEDFNINSPKQLGVVLFETLQLPVIKKTKTGYSTAVDVLEQLQ
                     GEHPIIDYILEYRQLSKLQSTYVEGLQKVISDDQRIHTRFNQTLAQTGRLSSVDPNLQ
                     NIPVRLEEGRKIRKAFKPTSKDSVILSADYSQIELRVLAHITQDESMKEAFINGDDIH
                     TATAMKVFGVEADQVDSLMRRQAKAVNFGIVYGISDYGLSQSLGITRKKAKAFIDDYL
                     ASFPGVKQYMSDIVKDAKALGYVETLLHRRRYIPDITSRNFNLRGFAERTAMNTPIQG
                     SAADIIKLAMVKFAQKMKETTYQAKLLLQVHDELIFEVPKSEVDSFSEFVEEIMENAL
                     QLDVPLKVDSSYGATWYDAK"
     gene            complement(19732..21219)
                     /locus_tag="O2701_000938"
     CDS             complement(19732..21219)
                     /locus_tag="O2701_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292843.1"
                     /translation="MKTIQLHKTTLILIAILLFYTFMGILLPLMHDDLQWFSNYNTDI
                     LKVGFASLNGRYIGNIFEIIAVHVSWLRWLSYGLISMGIIWMIMHITRCKAWTSYYLL
                     AFSLMLILPSAIYADTYGWFAGFYNYATSTLISLFIIYYCINAIIYKEKQPVSVTVLF
                     YVLCFFGQLFMENVTLFHCIILILAFLYEFTANRTLNYKLLFSFMIATIGTIIMFSNP
                     NYRKILFEGSEYQQVSNNQGIFSKFAEMISTSLPYGVIFSQIIILSMIAALIIYLLLR
                     SDRYVHLTILKRRIIMIGFITLPLYYLLFYNQFLLNKNTDIGLVGFVNVIVCGYFAFS
                     IFVGIYLSINDRKTQITLYSLLIAICVSASTLVIVTPIEPGNFLIVYTIHVIILIILL
                     KEFRKYKSINVNFIKGASLVLAVIYLSAFIYVHYEHEKRVQLLKQQIKDHPRQEVYTV
                     ESLPFEHYMHHPSPINKNYQEWFNNYEELPKMTKVKYIPFGSDDS"
     gene            complement(21718..23379)
                     /locus_tag="O2701_000939"
     CDS             complement(21718..23379)
                     /locus_tag="O2701_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HCZ4292844.1"
                     /translation="MKFHHRLMLLISSILIISFVTLGIIIHASITYVLSEKHEQALAT
                     DARSYIYLVQDNEIDKVKKIVKQQNIDLLITKHDKKVFSSGKLKAIPNSDSYIQKRDV
                     FIFNKKVNGYHVWIKGYNNDITEMHWTLWKYLILTCLVVLICLYFASRSFKRTLIRPI
                     QEVTYATQLLANGYYHIRVPESNVVETKALFVSTNDLARRLQKLNNEQKIQSNRLKTT
                     IENIPSAILMIDRNGKIVVANKAYYEQFNISHNIEQVGYHGYVNTEIEQLILESFKVE
                     KPIYEQLEVAINQVHAKYFDISCVPILTRSQKSLQGILVVMHDITNLKQLENLRREFV
                     ANVSHELKTPITSIKGFAETLIDGAKNDAESLDMFLNIILKESNRIESLVTDLLDLSH
                     IEQHTELDTDYMNLSDLTRRIIDNMMTQANQKNISIHTDIEKDVIVKAQESKIAQVIT
                     NLLTNAINYSYEDGDINVRVYRDDFRVIFEVQDFGIGIKLEDQQRIFERFYRVDKARS
                     RDSGGTGLGLSITKHIVEAHQGNIEVNSQVGKGSTFKVILKDYKE"
     gene            complement(23379..24083)
                     /locus_tag="O2701_000940"
     CDS             complement(23379..24083)
                     /locus_tag="O2701_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCZ4292845.1"
                     /translation="MSQKVLVVDDEHSIVTLLKYNLETAGYEVVVAFDGDEALEKVES
                     EQPDLIILDVMLPKKDGIDVCKTVRTNKNLVPILMLTAKNDEFDRVLGLELGADDYMT
                     KPFSPREVVARVKAILRRSQFVKEVEEIDDDITIGSIRIRPDYFEVYKHNELLELTPK
                     EFELLLYLIERQGRVITREHMLNTVWNYEFAGDSRIVDVHISHLRDKLEDNPKKPQLI
                     KTVRGLGYKLERPKEQ"
     gene            complement(24473..25741)
                     /gene="icd"
                     /locus_tag="O2701_000941"
     CDS             complement(24473..25741)
                     /gene="icd"
                     /locus_tag="O2701_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCZ4292846.1"
                     /translation="MTAEKITQGTEGLNVPNEPIIPFIIGDGIGPDIWKAASRVIDAA
                     VEKAYNGEKRIEWKEVLAGQKAFDTTGEWLPQETLDTIKEYLIAVKGPLTTPIGGGIR
                     SLNVALRQELDLFTCLRPVRWFKGVPSPVKRPQDVDMVIFRENTEDIYAGIEFKEGTT
                     EVKKVIDFLQNEMGATNIRFPETSGIGIKPVSKEGTERLVRAAIQYAIDNNRKSVTLV
                     HKGNIMKFTEGSFKQWGYDLALSEFGDQVFTWQQYDEIVENEGRDAANAAQEKAEKEG
                     KIIIKDSIADIFLQQILTRPAEHDVVATMNLNGDYISDALAAQVGGIGIAPGANINYE
                     TGHAIFEATHGTAPKYAGLNKVNPSSVILSSVLMLEHLGWQEAADKITDSIEDTIASK
                     VVTYDFARLMDGAEEVSTSAFADELIKNLK"
     gene            complement(25790..26911)
                     /locus_tag="O2701_000942"
     CDS             complement(25790..26911)
                     /locus_tag="O2701_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001662816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HCZ4292847.1"
                     /translation="MAELQRGLEGVIAAETKISSIIESQLTYAGYDIDDLAENAQFEE
                     VIFLLWNYRLPNEEELAHLKGKLNQYMTLNPRVYTHFEEYVTDHVHPMTALRTSLSYI
                     AHFDPDAENESDENRYERAMRIQAKVASLVTAFARVRQDKEPLKPNPDLSYAANFLYM
                     LRGELPTDIEVEAFNKALILHADHELNASAFTARCAVSSLSDMYSGIVAAVGSLKGPL
                     HGGANEQVMTMLSEIGSIENVDAYLDEKFANKDKVMGFGHRVYKDGDPRAKYLREMSR
                     QITKDAGREELFEMSVKMEKRMAEEKGLIPNVDFYSASVYHCMEIPHDLFTPIFAVSR
                     SAGWIAHILEQYKDNRIMRPRAKYIGETNRKYIPLEERK"
     gene            27259..28620
                     /locus_tag="O2701_000943"
     CDS             27259..28620
                     /locus_tag="O2701_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCZ4292848.1"
                     /translation="MAEKLQRELSNRHIQLIAIGGAIGTGLFLGAGQTIALTGPSILL
                     TYIIIGFMLFMFMRGLGEIIIQNTEFKSFADVTNTYIGPFAGFVTGWTYWFCWIITGM
                     AEVTAVAKYVSFWFPEIPNWISALFCVLLLMSFNLLSARLFGELEFWFSIIKIATIIG
                     LIVVGFVMILFAFKTQFGHASFTNLYEHGIFAKGASGFFMSFQMALFSFVGIEMIGVT
                     AGETKDPVKTIPKAINSVPIRILIFYVGALAVIMSIIPWQQVDPDNSPFVKLFALIGI
                     PFAAGLINFVVLTAAASSCNSGIFSNSRMLFGLSSQQQAPPNFSKTNKYGVPHVAIFA
                     SSALLLVAALLNYIFPDATKVFTYVTTISTVLFLVVWGLIIIAYINYSRKNPDLHKNA
                     TYKLLGGKYMGYLIFVFFIFVFGLLFINVDTRRAIYFIPIWFILLAFMYLRYKRIAAK
                     SNK"
     gene            complement(28653..>28892)
                     /locus_tag="O2701_000944"
     CDS             complement(28653..>28892)
                     /locus_tag="O2701_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCZ4292849.1"
                     /translation="IYSQRKIDVEPAFGFMKAILGFTRMSVRGINKVKRELGFVLMAL
                     NIRKIAAQRAVHYKIHIKKADFYQIINRNQLFYIA"
BASE COUNT         8925 a         5438 c         3950 g        10579 t
ORIGIN      
        1 ttttgacaaa atgatgaacg ttgaaaaatg ttatcaattg tgtactaatt aaaagtttcg
       61 tgcttacatt atttttaaaa tttttgtata agttttgacg tacaaaaact atgaaatata
      121 cggttcaatt aaattccata tttgttgttg tttattattt tgaattgatg aataacttac
      181 aattgtatcg tctgggtcca tatctaattg tgtcttaata tttttaatat gcttttgaac
      241 cttacctttt ggaattttgt cttctttagt gcatataact aaagtaggaa tatcaaaatg
      301 tttcaaataa ttgtacatta agatatcatc ttgtgttgga tcatgtctta aatcaactaa
      361 ttgaataact aattgcaaat tctctctctt agttatatat tcctcaatca ttttcccaaa
      421 tttttcacgt tgtgttttac ttactttagc atatccatac cctggaacat ccacaaaaat
      481 aagttgttca tctatattat aaaaatttaa cgtttgcgtt ttgccgggtt gctgtgatgt
      541 acgtgccata ttttttctgc caatcatact attgataaat gtagacttac ctacattaga
      601 tcgaccgctc agtgcaactt cagacaattc tgtttctgga tattgttctt cttttactgc
      661 actaatgatt aattctatat tattaggatt aactttcatt ttatatcctc acttttttat
      721 attctctttc aattctacca ttatataata tttgaacctt tcggtataca ttataaaaaa
      781 gctccgatca aagttaaact atttgagcgt caactttgat tggagctttt cacttttata
      841 acacatcaat gattaagctg atgttttact attattaatt aaattgcctt ctgcgtcgta
      901 tagttctggt tcagtttctt cattaattgt ttgtgctgta ataactacct tcgttacatt
      961 ttcgttagaa ggcacatcaa acataatatc gattaacgat tcttctatga ttgaacgtaa
     1021 accacgcgca cctgtttttc tttcaattgc tttttcacta attgctgata aagcttcttc
     1081 agtgaactct aaatccacat catctaattc cagcatttta gtatattgtt tcacaagtgc
     1141 atttttaggt tgcgttaaga tgtttttcaa cgcagttaca tctaatgttt ctaaattagc
     1201 tacaattggc acacgtccga taaattcagg aatcaaacca taggcttgca aatcttctgg
     1261 gcgaatttgt gctaataatg cttgttcgtc atatttatca gcttcattgc ttgagaaacc
     1321 aataactttt tcaccaagac ggcgcttaat cacttcttca ataccatcaa aggcaccacc
     1381 aagaataaat aagatatttg ttgtatcaat ttgaatcatt tcttggtttg gatgtttgcg
     1441 tccaccttgt ggcggaacac ttgcagtcgt accttctaag attttaagca atgcttgttg
     1501 aacaccttca cctgaaacgt cacgtgttat agatgtgttt tcagatttac gtgcaatttt
     1561 atcaatttca tctacataaa taataccttt ttcggcttta tcaatgtcaa agtcagctgc
     1621 ttgaattaat ctcaacaaga tattttcaac atcatcgcct acataaccag cttcagttaa
     1681 acttgtcgca tctgcaattg caaatggtac attcaacgtc ttggctaagg tttgagctaa
     1741 taatgtttta ccactacctg ttggcccaat taatgcaatg ttactttttt gtaattcaac
     1801 atcatcttct tttggtccta attgttgaat acgcttatag tggttataaa cagctacagc
     1861 taaagatttt ttagcttttt cttgaccaat aacatattcg tttaaatgat ccataatttc
     1921 tttaggagta ggtaattctg tcatcgcttc agaagtgttt tgagctaatt cttcttcgac
     1981 gatttctgag cataattcaa tacactcatt acaaatatat acaccacttc ctgctacaag
     2041 tttttttact tgatcttggt cttttccgca gaaagagcat ttcaaatttt cttcatcttc
     2101 attgaattta aacattcttt ttacacccct attcgttaaa gactatacta gattggatgt
     2161 tacaatgcaa catattaaca tacaaacttt ttgcttaaag aatagtagca gatacataag
     2221 ctatgcatct gctactcatt tttgtaattt aatatttaat gaagattaat cttctttagt
     2281 tccttcaacg aactttgcgt tatctcttaa taaatcgata actttttgga tacgaacatc
     2341 atttttaatg atatcagtat tacctaaagt atttttgata tcttcaactg agatattaaa
     2401 ttgtttactc attttttcta attctttatc gatatcttca tcagtagctt cgattttttc
     2461 agcttcagcg atcgcagtta aagttaagtt agttttaaca cgttgttctg catcgtcttt
     2521 catttgctct cttaattgag tttcatcttg acctgagatt tggaagtacg tttgtaaatc
     2581 taaaccttgt tgttgaattc tttgtgcaaa ttcagacacc atacgatcta attcagtatt
     2641 aaccattgct tcaggaatat cgattgttgt attatcagta gcttttgtaa tcgcttcttc
     2701 tttttcaaca ttttcagcat ctgtagcttt ttgttcagct aaacgtttac gtaagttttc
     2761 tttgtactcg tctactgtat ttgcttctgc atctaattca ttagcaattt catctgttaa
     2821 ttctgggact tctttaaatt taatttcgtt aacttttgtt ttgaaagttg cttctttacc
     2881 ggctaattct tcagcatggt attcttctgg gaatgttacg acaacatctt tttcttcgtc
     2941 aactttcata ccttctaatt gctcttcgaa accaggtatg aatgaacctg aaccgatttc
     3001 taaatcgtaa ccttcagctt gtccaccttc gaattcttct ccgtcaactg aaccactaaa
     3061 gtcgatgtta actgtgtcgc cattttcaac aacaccatct tctttaacga ccatttcagc
     3121 taaatgtcct aagctgtggt caatcgcttc ttgtaactca tcatcagata attcagtttc
     3181 ttgtttttca atttcaagac ctttatagtc tcctaattta acttctggct caactgtaac
     3241 tgttgcttca aaaatgaaat ctttaccttt ttcaatttga gtaacactta cttctggttg
     3301 tgcaactggt ttaatatcag tttcgtcaat tgcttcacca taagcatctg gtaataaaat
     3361 gtcgatagca tcttgatata atgcttctac accaaagcgt tgttcaaaaa ttggacgtgg
     3421 cactttacct ttacggaatc caggtacgtt aatttgttta accacttttt tgaatgcttg
     3481 atctaacgct ttgtttactt tttctgcagg aacagtaaca gttaataaac cttcgttacc
     3541 ttcctttttt tcccaagttg ctgtcatgta tatatacctc catgattaac taatttattt
     3601 tttcaacttc cctattatat cataggtcta ttccctatac aaacattgaa atcacaacgt
     3661 ttatatattt gtaaatcaac ttttttcgtc aaaactatat taaaatgctg agtattattt
     3721 tgcaacaatt taactattat tattcaaatc taattgctga ataaaattaa atgcatcgat
     3781 actttgtaat tcacatttaa gtcctaacat atttttgaaa taacattcgt atgcattaat
     3841 ccatttattc gtttcaaaca aagtttcaat atcaattgga taaatcatga tagagtgatt
     3901 attcataata tggtgtgctt cttcagcgat atgtaaagca ccatcattta atgtttccat
     3961 aacgttaggt ataacttttt ctttaagtgt tgtatgttct agccctttta aattagctgg
     4021 tacaaaagtt acatccatac catacttttc aattgtcagt tcttgtgtac aatttgcgaa
     4081 ccttaaatac tcaatcatta aactaatgag gttataactg tacgtattag attttaatat
     4141 atataatacc gtttcttgaa attgaaaatg accattgtct attaacttca aaatcaagtg
     4201 cgtctgttcc ctcattgata acgtatcaaa atcagctaat gactgagtca atcgtttttc
     4261 atcttcaatt aattttgact tagcaaattc tttaagagga tgtaatgcca ttcttgtttt
     4321 gtgatctttg acttcatcaa taatttgatg aattacttct accgcttcaa aatattgtcc
     4381 caaaccaatc aaacttttca cgtaataaat catcaatgca tcatattgtt gtatgccagt
     4441 tttcaataag acaattgttt cttctcttaa ttcaagaaat gaccccgtct catataacat
     4501 acggcatttc atcattgcta tttcttcagt gagttcaaat tgacgttcat attgcacaat
     4561 atattcatac atcaggtcat agttttggtc ttgttcagca cgttttatat ctttatataa
     4621 ctttttcgaa gagttaggaa actgaataat atctgacata tatgtcacct cgtttgatta
     4681 taaatcttga aggattaatg cactccacgt ttcgaaatca tcatatgact ctatgttgcc
     4741 tttttcaacc cattttattc gtaaagtatc tgtttcttta gcttctacat cattcgtact
     4801 tacagtgatt ttaaatacaa caccaatatg taccttgccc acttcattat tatcgtcatt
     4861 aataaaaccg atatattcca tattttgtga atcttgctca cttaaaccta cttcttcttc
     4921 taattctctc tgtgcattaa ctctcaatac ttcgttaata gattctgctc ctggaacatc
     4981 attcatatga ccgcctacac ctattgaaga ttgtccatgc aatcgagctt ctccaccgcc
     5041 agataatcgt tcatacacta atatctcgcc atgctcattt tcaagtaaac aataagaaat
     5101 gagttgttta tatgatggat cctcttccat atcgccgcgt cgcttaactt catattgact
     5161 tagcgcatca aaaatatttt gaccttctgg tttattctta tttaaaaacc cattgaaagt
     5221 attcttttca ttattaaaaa taatttctct aggtactaca atgatttgtt catcaaattt
     5281 agacatcgta taaaactcct cactttaatg tttgttctct taaatgcatt ttagcaaata
     5341 tgtcatattt tagcgattaa atgtggcatt ttgctattta aaaaataaaa aagagatgac
     5401 aatgccatct cttttttata agtgttatta ttttaaagca tctttagctt tagttactaa
     5461 ttgagcaaat gctttttcgt cagaaattgc gatttctgat aacattttac ggttaatgtc
     5521 gataccagct tttttcaaac cgttcattaa acgtgagtag ctcatttcat gttgacgagc
     5581 tgctgcgttg atacgtgtaa tccataattt acggaagtca cgtttacgtt gacgacggtc
     5641 acggaaagca tattgacctg atttcattac ttgttgctta gctactttgt ataatgtatg
     5701 ttttgaaccg aagtaacctt tagctaattt aatcgttttt ttacgacgcg ctcttgttac
     5761 tgttccacct ttaactcgtg gcataaaaaa ttcctcctta ggtaattcct accgatttct
     5821 gtatttgttc ttattttttg tatgctaata attgttttac acgtttcata tcgctcttag
     5881 acactaatct agctttacgt aattgacgtt tttgtttagt gctcttgttt gcgaataagt
     5941 gagatgtgaa agctcttgaa cgttttaatt gacctgaagc agttctttta acacgtttag
     6001 ctgctccgcg gtgagttttc atttttggca taatataagt cctcctgtaa tagctttaat
     6061 tatttttcag ctgttggcgc taacatgata aacatttgac gcccgtccat tttaggtttt
     6121 tgttcaactg ttgctatatc tttgcattca tctgcatatt tttctagcac acgttgacca
     6181 atttccttat gcgtaatggc acgccctctg aaacgaatag atactttaca tttatcgcct
     6241 ttagttaaga atttacgtcc gtttttcaac ttcgtttgga aatcatgttc ctcaattgtt
     6301 ggacttaaac gaatttcttt aacattgata attttttgtt tctttttcat ttctttttct
     6361 tttttctgtt gttcgaattt gaatttaccg taatccataa ttcttgcaac tggtggtttc
     6421 gcattcggtg caacgaccac taagtctaaa tctacacgtt cagccatttc taaagcttca
     6481 cgctttgatt taacaccaat ttgttcacca tcttgaccga ttaaacgtaa ttcttttgca
     6541 cgaattttgt cattgatttg agtttgatct tttgctatgg ttgacacctc caaaattttt
     6601 acgaaatttg caccaagcaa aaaggaagag caggtataaa atacccgctc ttccttatac
     6661 acagttatgt gtaatgtgat taacctgcca actgctttat gcgtcgctac aggtgagaag
     6721 cgggtgcttc tacttggttc gtttcgtatt caacgttatt aatcatatca acaattcaca
     6781 tttaagtcaa cactataact gtaattattt ttattttaac cttttatttc atccattgac
     6841 acgtcttgac gtaaatctac ttgttctaat ggaatttttt tcgttttata tcgaagctta
     6901 tgataaataa agaatgctaa aaatactgga attcccatat acgtaattaa gaagcgacta
     6961 aaattaaaat ctcctgtttt aataaagtca acatcttgac caataattac tacaatacat
     7021 aaaaagccag caaataatgg tccgaatggg aataatttag cagtatattt taatttagat
     7081 ttgtcataat tttgtttatc aaatgctctt ctaaatcgat aatgacttac tgctatacct
     7141 acccaagcaa taaaaccagt taaaccactt gctgcaacga tatattcgta tgcacctttt
     7201 gtaaggcttt gtaatacgaa aattatcact acaatgattg ctgtaacgag taacgacata
     7261 tatggcacac cgtttttatt tgttttacca aatgcttcaa acgctaattt atctttactc
     7321 attgaatata gcattcgagt tgaagcatac atacctgagt tacctgctga taacacagac
     7381 gttaaaatga ctgcattcat aaatgatgct gcaaacgcaa atccagcatt tttaaacact
     7441 aatgtgaatg gagacgttgc tacattatca ctacccccca ttaatgcact actatcataa
     7501 ggaattaaca taccgataac aaaaatggct aaaatgtaaa ataataaaat tctccagaat
     7561 acttgtttaa ttgctttcgg cacagcacgt tcaggatttt ctgattcacc agccgtaata
     7621 ccaattaact cagtaccttg gaatgagaaa ccagcgatta agaatacacc tagaattgat
     7681 aacaaacttc ctcctaagtt gccaccaaga attggacctt cacctttatt aaatatttcg
     7741 aatcctacaa catgaccacc catgattccg acaatcgtta ataaaccaat tgcaatgaaa
     7801 acaataactg taaccacttt tatcaatgcc aaccagtatt cactttcacc atagacgcga
     7861 actgataacg aattcagact aaaaattata actaagaaca acgcactcca tgcccaagcg
     7921 ggtatgcctt gcaatggtgt ccaatattga atgacttgtg ctgcaatcgt aatatctgct
     7981 gctacagtca ctacccagtt aaaccaatag ttccaaccaa gcgcaaaccc taaagatgga
     8041 tcaacaaatc ttgtagcata tgtactaaat gaacctgata ctggcaaata cgtagccatt
     8101 tcgccaagtg acgtcattaa gaaaaatacc attattccga taattgcgta tcctattaat
     8161 gcacccaaag cacctgcatc atgaattgct ccaccagaag ttacaaataa acctgtacca
     8221 atacaacccc caatcgcaat catagaaata tgacgatctt taagtcccct tttgacaaca
     8281 ttgttacttt cattttgaac ttttgacata taagctccct ttccatgttt tagaggccaa
     8341 gaaataaata acctttattc actttcatac ccttttttac agaacatagt gatattaaaa
     8401 gtaaaaaagt ggctacatac ctttaagata tgcaaccact ttggcaatta tctatataag
     8461 gtagcacatc acattctgtg acagtacagt tcctattcga aaactgtccc aatagatata
     8521 atttatggtt tatatctatt tcggcatctt tacctttcac ttgttcaact tatgtaccat
     8581 aaatacttct gacaagttac taattaaaca tgcaacctct aactcaattt aatattttaa
     8641 ctaacttgta atatacagga ttcatcacgc ataatcaacc ctgtaaaact tgatacgcaa
     8701 taaaagtttt aaagcatttt attgcgacaa ctgtctatct atgttttttc aaacgaattt
     8761 catcaactag attccagata aattcatctt tttcaactgt ttcttggtct tgcgatccat
     8821 attgacgcac attcacttga ttattttcaa cttccttatc cccaactacg atttgataag
     8881 gtattttttg catttgagct tctctgattt tataacccat tttttcatta cggtcatcaa
     8941 tacttacacg aacgccttga gatttcaatt catcttgtaa ttggcgcgca taatcataat
     9001 gtaaatcaac gttaactgga atgatttgaa cttgttttgg cgctaaccaa gttgggaatg
     9061 cacctttagt ttcttcagtt aaaaatgcta caaatcgttc cattgttgat acaacaccac
     9121 gatgaataac aactggacga tgatgttcac catcttgacc aatataagtt aaatcaaaac
     9181 gttctggtaa taagaaatca agttgtgctg ttgataatgt ctcttcttta cccatcgctg
     9241 ttttaacttg aacatctagt ttcggaccat agaatgccgc ttcaccaatc gcttcttcgt
     9301 acgataagcc aagctcatcc gctgcctctt taagcatatt ttcagcttta ttccacatat
     9361 catcatcatc aaagtacttt tctttatctt cagggtctct ataacttaat ctaaagctat
     9421 aatcctcgaa accaaagtct ttatacacat caataatcat gtttacaacg cgtttgaatt
     9481 cttctttaat ttgatcaggt cgaacaaaga tatgtgaatc atttaaagtc ataccacgaa
     9541 cacgttgtaa tcctgataca gcaccacttg cttcatatct atgcatcgtt cctagctcag
     9601 cgatacggat aggtaattca cgatatgaat gtggtttatt cgcataaatc atcatatgat
     9661 gtggacagtt cattggacgt aataccatag attcagtttc atctaactgc attggtggga
     9721 acatatcttc ttgatagtga tcccagtgac cagatgtttt gtataaatca acattagcaa
     9781 gtactggtgt ataaacgtgg tcatatccca tgctaacttc tttatcaaca atgtaacgtt
     9841 caatttcacg tctaattgtt gcaccgttag gtaaccataa tggcaaacca gcaccaacta
     9901 attggctatt tgtgaatagt tctaactctt taccaatttt acgatgatca cgttctttac
     9961 gctcttctaa catttgtaaa tgtgctttca attctttttt atcaaagaaa gcagtaccgt
    10021 atatacgttg taacattttg ttgttactat ctccacgcca gtatgcacct gctgtagata
    10081 atagtttaaa ctctttaatt ttagctgttg atggaacgtg aactccacga cataaatcag
    10141 taaaatcacc ttgactatat aatgttacat tttcatcttc aggaatcgcg tcgattaatt
    10201 ctaatttgta ttcatcattg ctgaataact ctttcgcttc atctcgtgaa accacttttc
    10261 gttcgatttt catattttcg ttaacgattt gtttcattgt tttttcaatt tgttcaaagt
    10321 catcagatga gatgttttgg tcaatgtcga agtcatagta gaatccacct tctattacag
    10381 gacctacacc aaatttaaca ttaccatata accttttaat cgcgtgtgcc attaaatgtg
    10441 cagtagaatg acgtaatacc tctaacgctt cttcactacc tggtgtcaca atttcaattg
    10501 atccatcagt ttcaagcggt ttagttaaat ctacaagttg cccgttaaat ttgccggcaa
    10561 cagctttttt acgtaatcca ggactaattg attgtgctat atcttcagta gtagtacctt
    10621 tatcaaacgc ctttttatta ccatctggaa attgaatatt aatttgttcc atgttaaacc
    10681 ctcctatttg ctttataaat acttttatct gacctcgtta aaaaattgaa caacaatcat
    10741 cgttttaaca ttaaacctat taacaataaa cagggaattt atgacaccaa caaacattta
    10801 tgtctgaaaa aaacaaaaaa tcccgcccct atacaaggga cgagatcgtc gtggtaccac
    10861 cctagttatc taatacaatt tatgtattaa atatctctgc ttaagataac ggtcttgatc
    10921 cgggtattca ttacaaatac cataaatgaa gtagtaatca tctaatttat taaccatatt
    10981 ctcatcaaac aatggctttc tgtgtaatat tgattagatc atcttgtctt caatcattta
    11041 aacgatttag atttgtattc attatacacc aatcatttta aaattcaatt gtttctgaaa
    11101 ttttctcctg ataaaaagta tggtgttgac aaagatttga cacgttcaat aatacgtgct
    11161 gctttagtct tctcttcacc atcacgagtc atcgctaaat gatgttccaa ttcactatag
    11221 tcaaaattag aactaaagaa tgttggtaat tcatgaacca ttcgataatg tagcaaaggt
    11281 ccaattacct catctctcac ccatggagtc acttcttcag ccccaatatc atcaagcatt
    11341 aaaatgtttg cttctcttac gcgatgtaat ttcttttcaa aagaaccatc tttaaagcca
    11401 ccttttaatg ttctaataaa ttccggtaaa taaataattg tcgaacgtac cttcttagat
    11461 ttgagctgat tcgcaattgc acctagaata aaagatttac ctgtcccaaa tggaccataa
    11521 aggtaaaggc ctttcacttg ttccccatta gttattgctg tacaaatatc atctgctgcc
    11581 atagctacat caagacggtc tcgatgattc atataaatat ctttcaattt ggcatttaaa
    11641 gtatctcgtt gcatatgatg agatgtaatt agctcagctt caaagcgttc ttcgtcgtac
    11701 ttgattttac atgggcattg taaatagcgt attttaattc ggttattatc aacatataac
    11761 tcaggcacat gcccctttac gaaatttgga caatcagcaa atttatgacc gtcataatgt
    11821 ttttgttgat ctttatactc ttgtaacaca tttaagtctt catcaatcat agcattcgtt
    11881 aattcagctc gatgcgcttc caaaaattgc ttaacatctg ggtcattgat tacttctttt
    11941 tttatctttt ctattctttt ttcaaagtcc tgcgacgtgt taattatact tttaaattgc
    12001 ttcattattg actgtcctcc tcccattttt tagataattt atctagaaat gcttgtcgat
    12061 cttgctctaa ttgttgatca tctacgctat tatctttagc cgaatcttct tcactaggtt
    12121 tatctctatt ttctaaccat ttaggtgttt tttcttttga aatacgatta cgctgcccat
    12181 agtatgaacc acgcttttgg taatttccgc tagaaccctc atttttaggt tgattaactt
    12241 ttttagcgta attatatgct tctttagctg tcttaatacc ttttttcttc caatttgatg
    12301 ctatttccaa aatatacgct ttaggaagtt tcatatcttc ttttaacatg acaaattgca
    12361 acaaaatatt aatgacgcca aaagacattt tttcacgttc aattaattct tcaaccattg
    12421 tcttttgcga tatagttggt tctgattcag accaagaagc taacatatca attggactcg
    12481 tttgttcaag taactcaaac cattcatcac tttgtggctt tggattcact tctgaagatt
    12541 tgcccgccga agatgatgta gcaggagatt tcacctgtaa tttaggcatt tgattttcgt
    12601 gttccattaa gtaatacgag cgtgcttgtt tacgcatttc ttcaaaggat aactgttgtc
    12661 cacttgtaat tgaatttaaa ataacatgct tcatgccatc tgctgttaaa ccatataaag
    12721 tcgcgagttg tgtaattaaa cgctttgcat ctttggtaac aatgtcttga ctaataaaat
    12781 gtttacctaa catttgtctc aacatttcaa agtcaaaaga ttcatttgat aaatcgatac
    12841 cttggtacgg ttcattaatc ggaatatcac ttgtatcgat atctattttt gtagacggca
    12901 ctttaaaaac atcagtaaat tgtcttgtta cctgtttaaa ttcactcaaa tcaatttgtt
    12961 gatactcaaa gtatttcttc aactcatgaa atcgacgatg ctcgacttca ctatataaaa
    13021 agattgacaa cattggatca ttaaaaaata aatgtgctga aggcggttga attaattggt
    13081 aaacaaattg tgtttcttgt tcatcatgtt tgacaaacac ctttaacaat ccaatcgctt
    13141 caagtaagtc catttgttgt ctaaactcta gtaaattaat tttaagttca ttcataaaaa
    13201 tataatgaga aagaatcaat gtttcattat gactttcttt aacgaattga gtcataaaat
    13261 gatataaacc cactgcttgc gttccaatta gcggtgtata cagtcgattc aatacctcta
    13321 aatgattcgt atttaaatca aagtgttgca taactttgaa ttgatccttt ggtcttaagc
    13381 cgaattcgaa ggcttgtcgt cccatttaag catcactccg tttgttttcg cttaaaatcc
    13441 cttgcatcga tgctaacaat tgatcaacat ctttaaattc tttatagact gatgcaaatc
    13501 taacatatga aacttgatca acatgcatta acaagttcat aacgtgttca cctatatctc
    13561 gtgaagacac ttccgtatga ccttcatctc gtaattgcca ttcaaccttg ttagttatgt
    13621 cttcaagttg ttgatatcta actggtcgtt tctcacaaga acgcacaagt ccattaagta
    13681 tcttttctct tgaaaactgc tctcttgtgc catctttttt cacaactata agctgactaa
    13741 cttcgatatg ttcaaatgta gtgaaacgtg ttccacaatt ttcacattct cttcgtcttc
    13801 gaatggcatt taattcatcg gcatgccttg aatctacaac tttagattgt gtagaattac
    13861 atttcgggca tttcattaca tcaccctctt tattttgatt atgcctaatt atactataaa
    13921 tctagagatg aaaaaagaat ccctcaattt aattcattta accaaataat gaaacaataa
    13981 aaaacattat atcgttactt attaagtaat ttgcatgaca atattattgt attaaaaata
    14041 aaaaacctaa ctccgaagtc agagttaggc tataaattaa ttgtattaac ttgcacttac
    14101 agtttctttt gatgtcaaaa gtgctccaat ttgctcagca acatctacaa ctctatttga
    14161 ataaccccat tcattatcat accaagcaat aacttttact ttattccctg acatgaccat
    14221 tgttgatttt gcatcaataa tagctgaatt tggattagta ttaaaatcaa cagacactag
    14281 tggttgatgt tcgacttcta tgataccttc taaacctgca ttttcaaaag cttggtttac
    14341 ttcttctgca gttacttctt tttctaaatc aacaactaaa tcaacgagcg atacattctt
    14401 tgttggtaca cgtaatgcca tgccgtgtaa tttaccttct aattctggta atacttcttt
    14461 taaagctttc gccgcaccag tagaagtagg aataatgctt tcattacatg aacgtgcacg
    14521 tcttaaatct ttatgtggat tatcaatatt tttttggtca tttgtaatag cgtgaacagt
    14581 agtcattaaa ccattaacta ttccaaactg attatttaaa acttttgcaa ctggaccaat
    14641 gcaattagta gtacatgaag cattactaaa aatgtcaaat gcttctatat ctaattggtt
    14701 atcatttacg cctttaacta ccatttgaac atgtccacct tttgaaggac cagttaacaa
    14761 aacttttttg gcacctgctt taatatgtgc gatggcttta tcaccatgat taaatttacc
    14821 agttgcatct atagcaatat cgatatctaa ttctttccat ggcaagtttt caggattgcg
    14881 atcagcaacc aatttaattt tatgatctcc aacttgcaat ccattttcaa tcggttcaac
    14941 ttttagatta tattttccat gtgtcgtatc gtaattgatt aaatgtgcaa ttgtttcggg
    15001 tggataacta gcatttatcg ctactacatt taaattttta ttttgtaatg caatacgtaa
    15061 taccattctt ccaattctac ccataccatt aattgcaata ttcgttgaca ttaaaagcac
    15121 cccttgatct ttatgtgtta tactttatgg aattagtata gcatattata tgaaatttga
    15181 aagcgttttc cgttgattta tttatctttt ttataaaaaa tagtcattac ttacatacat
    15241 caacgcgtac gtaaagtaat gacttattta tagtgtaata ttaatcttct tctccgtaat
    15301 tcggcttttc aatataacct tcttcttcta acaatctctc aaggttttgt tttaattcaa
    15361 gtttatcccc taaattatcg ataacatgat cggccattcg gcttttttta tcaatagaaa
    15421 tttggctata gacacgtgct ttcgcatctt ctaatgacaa attattacgt tgcattaaac
    15481 gatccatttg tatactttca gaagtgtata caacccacac ttcgtctact gtattttcca
    15541 attcattttc aaataataat ggaatatcca tgattacatt atatccttgt tttaaatatt
    15601 cttgcttttc ttcttccata atatctcgca cgataggatg tatgatagca tttaattcta
    15661 agcgtttttc tggatgatta aacactagat cacccatata acgacgattc atctcaccat
    15721 tttcatcaat tgcttcatca ccaaagactt ctcgtacttg agctaaacct ttactcccct
    15781 ttttaacagc ttccctggct gctttatcag catctactac tttaaaaccg aatacggata
    15841 agagttctga tactgttgat tttcctgagg cgattccacc tgttagacca ataactttcg
    15901 gcataatttc actctttctt tatttttgac atactggaca ataatgacta tttcttgtcg
    15961 cgatgatttt tgtttcaatt tgacttccac acactttgca taccggctgc ttatatacat
    16021 taagatgcaa ttgcatctca ccagtttttc catcagcatg acgataatct gaaatacttg
    16081 taccgccata tttaatacct tcttctagta cttctctaac ataataaaaa accatttctt
    16141 gttgttggtg tgttaagtct tttacttttt tatctggtaa aacacctgca cgaaacaacg
    16201 cttcacatgc gtaaatattt ccacaacctg cgattacttt atgatccaaa atcacttgtt
    16261 tgattggttt attcttatta gactgttgat gaattcgatt taaataatac gtcaatgctt
    16321 catttgaaaa aggttcaggc gctatttcta aaaatgaagg ataagatgct acagacgcaa
    16381 catttctaat ttctccaaaa cgacgtatat ctgaataaat taactttttg tcatttgaca
    16441 actcaaaaat aacatgccaa tgcttacgat aattaggtat cataatatct tcaagttcat
    16501 ctacaatgaa aaaaccgccc gccataccta aatgactaat taatgtacgt tgttctcgtt
    16561 tattatctag ctgaaaaacg atatatttac ttcttcgttc tacatttgta atggtatagc
    16621 cttccgataa agttttaaaa gtatctaatt caattccttt tataattgtt tccttgcctt
    16681 gagctttacc ttcgattact ttatccgaaa atataacgtg ttcaattttt tgatttataa
    16741 cgtagggttc aattcctctt tttacatgtt ctacttctgg taattcgggc ataccattaa
    16801 cctcacttta ttttgcatca taccaggttg caccataact tgagtctact tttaatggaa
    16861 catctaattg caatgcattt tccattatct cttctacaaa ttcactaaat gaatctactt
    16921 ctgacttagg tacttcaaaa attaattcat cgtgtacttg taataatagt ttagcttgat
    16981 atgttgtctc tttcattttt tgagcaaatt taaccattgc cagtttaatg atatctgcag
    17041 cactgccctg tattggcgta ttcatagcag tacgttcagc aaagccgcgt aaattaaagt
    17101 tacgactcgt aatatcagga atatagcgtc gacgatgtag caatgtttcc acgtaaccta
    17161 aagctttggc atcttttaca atatcagaca tatattgttt tacacctggg aaactagcta
    17221 aataatcatc aatgaatgct tttgcttttt tacgagtaat acctaaactt tgacttaaac
    17281 cataatcact tatcccataa acaattccaa agttaactgc ttttgcttga cgacgcatta
    17341 aactatcgac ttgatcagct tctacaccaa atactttcat agcagttgct gtatgaatat
    17401 catcgccgtt gataaatgct tctttcatac tctcatcttg tgtaatgtgt gctaatacac
    17461 gcaattcaat ttgagaataa tctgctgata atataacgct atcttttgaa gttggtttaa
    17521 aggcttttct tattttacgc ccttcttcaa gtctaaccgg aatattttgt aaattaggat
    17581 ctacacttga taaacgtcca gtttgcgcta aagtttgatt aaaacgtgta tggatacgtt
    17641 gatcatcact aattaccttt tgcaatcctt caacataagt agactgtaac ttagataatt
    17701 gacgatattc taaaatataa tcaatgatag gatgttcacc ttgcaattgc tctaagacat
    17761 ctacagcagt agaatatcct gttttcgtct ttttaataac aggtaattgt aatgtctcaa
    17821 acaatacaac acctaattgc ttaggagaat ttatattaaa atcttcacca gctgcatcat
    17881 ggatatttcg aatcaagacg tctaattttt cttgaatttc tttttccatt tcttctaaat
    17941 catgaacatc tgtaaatata ccaatttctt ccatttcact taaaatctta gctagcggta
    18001 gctctaaatc agccaagagt tctacctgat tgtattcttc taattgttta tccatatttg
    18061 gtttcgcaaa gtaaattgca tcagtaatag aagcaacata tggatttaaa acatcatctt
    18121 caggtacctt aaatttctta ccttttccat atatactcac atcgtctttc acaaaacttt
    18181 gaccgtacaa tgaaacaact gattgaacat cactaatcgt acgagatgga tcaattatat
    18241 aactggccaa cataatatcg aaagaaatat tttgaatatc aatccccaat ctatgtgatg
    18301 ctacatatgt ttttttagca tcatatacga cttttttcga attcggattt tctaaccatg
    18361 aaactagttc gacataatta tttatgtcat ccgcattaat tacaatatgt ttctcacctg
    18421 taaataaaga gaattttaaa atattatttc gcaaatagtt accaccgtct aattcgaaat
    18481 ggatggccgc ttctttcaat gaagtaaaat caatattatc aaaagacgtt tcaatttcaa
    18541 atgtcttttc tattgcatct tcaacgcttg ctgattgatc aatgtcagcc aacaattgtt
    18601 tgaattctaa cttcttaaac aattcgattt tttcttgttg ttcatcttga tgagtcatta
    18661 acgtatcttc aagttttact tcaatcggac tatctacatt aatcgttgct aattctttac
    18721 tcattaatgc atcttctttg ctattttgaa gtttttcttt taacttttta cctgaaattt
    18781 catctaaatg ttcatagaca ccttctactg tgtcaaattg gtttagcaat tttattgctg
    18841 ttttctctcc aacacctgca acacctggta tattatcaga agtatctccc attaatcctt
    18901 tcatatcaat aatttgatta ggtgttaacc cgttgtattt ttccgcaata aagtcaggtg
    18961 tataatgatc aacatcagta acaccttttt tagtgtaata aatggttaca ttatccgttg
    19021 caagttgtgt taaatctcgg tctcccgtaa taataattgt ctgaaagccc gctttatctg
    19081 cttctttact taaagttccg ataatatcat ctgcctcata gttatctaat tcataacgtt
    19141 taatatgata agcatctaat aattggcgaa tataaggaaa ttgctcactt agttcaggcg
    19201 gcgttttctg gcgtccacct ttatattcac tatatttttc atgtctgaaa gtcgttttac
    19261 ctgcatcaaa cgctactaaa aaatgatttg gcttttcttc ttttaaaatc ttctctagta
    19321 acattgcaaa accatatact gcattggtat gaatgcctgc tttgtttgat aacaaaggta
    19381 atgcataaaa agctctaaaa cttaagctat taccatcgat taatactaat ttattcacaa
    19441 ttttaacctc cagaactaat ttatatatat tttagcatat tcgactgtga tgtcgtaatt
    19501 aaagaaacta tctcatagag tatttataca cgacctaatt tcaaataaaa caagtgccta
    19561 tatagactta gctaaaaaat aaaaaagaca taatcatgat tgttcttgat gagcactcca
    19621 taaagtcagg ttaaaagtcc aacttttgag atgcacatta tcatttcatg attatgtcta
    19681 ctttttaata tttttatgct gctattttaa atttttatag gtaagaataa gttatgaatc
    19741 gtctgaacca aatggaatat atttaacttt ggtcattttc ggtaattctt cataattatt
    19801 aaaccactct tggtaatttt tattgattgg tgacggatga tgcatataat gttcaaacgg
    19861 caatgattcc actgtataaa cctcttgtcg tggatgatct ttaatttgtt gttttaataa
    19921 ctgaactctt ttttcatgtt cataatgaac ataaataaat gcactaagat atatcacagc
    19981 taaaactagt gatgcacctt taataaaatt aacattgatt gacttatatt tccgaaattc
    20041 ttttaataaa ataattaaaa tgattacatg tatcgtataa acaatcaaaa aattacctgg
    20101 ttctattgga gtaacaatga ctagtgtcga cgccgaaaca catattgcaa taagtaagga
    20161 atataaagtg atttgtgttt tacgatcatt aatagacaaa taaataccta caaatatcga
    20221 aaacgcaaag taaccacata caattacgtt cacaaaacca accaatccaa tatctgtatt
    20281 tttatttaat aaaaactgat tgtaaaatag taaataataa agcggtaaag tgataaatcc
    20341 tatcataatg atacgacgct ttaatattgt taaatgtaca taccgatcac ttctaagcag
    20401 tagataaata ataagcgctg caatcatact taaaattata atctgactga aaatgacacc
    20461 atatggaagt gacgttgata tcatttctgc aaattttgaa aagatacctt gattatttga
    20521 aacttgttga tattctgatc cttcaaataa aattttgcga taatttggat tggaaaacat
    20581 aatgattgta cctatagtag caatcataaa tgaaaacaat aatttataat taagtgttcg
    20641 atttgcagta aattcataca gaaaagctaa aattaaaatg atacagtgga ataatgtcac
    20701 attctccata aataattgac cgaagaagca taaaacatag aatagtacag taacacttac
    20761 cggctgcttt tctttatata taattgcatt aatacaataa taaataataa agagtgaaat
    20821 tagtgttgat gtcgcataat tataaaatcc tgcaaaccag ccatatgtat ctgcataaat
    20881 agcacttggt aaaattaaca ttaaagaaaa tgctaacaaa taatagcttg tccacgcttt
    20941 acaacgtgta atgtgcataa tcatccaaat gatgcccata ctaatgaggc catatgaaag
    21001 ccaacgtaac cagcttacat gtacagctat aatttcaaag atatttccga tatagcgacc
    21061 attaagtgat gcaaatccta cttttaaaat atcagtatta taattgctaa accattgtaa
    21121 atcatcgtgc atgagtggta gtaagatacc cataaaagta taaaacaata atatcgcaat
    21181 taatatcaaa gttgtcttgt gtaattgaat tgttttcact ttgctaatcc tcaaatctag
    21241 ttaaattttc ctcaacttgt aggtcgaaaa attaattcaa tatttttaat gtatttctaa
    21301 ttttcaccta tgcatgtttc ctcaatcaaa ttagataaac aaggtattta atattacttt
    21361 caacaattta tctaaatcgc ccctcgtctt tttctatgac gaatgattac acttgtctat
    21421 tcaagtgttt aaattaagat ttatttccgt tcaaataaaa cttattactt tttaattaag
    21481 catctatttc aagcgcgtta ctaaaatttc tttattataa taaatatatt ataaaattgc
    21541 ttgattcaca gcaatctatt acttataact tacaaatcga catatacaaa tgacaaaatt
    21601 gacaaccgaa tttattccac ttcattaact ctaatttaat accaatagag atttgaacga
    21661 aataaaaaag cagcaacaac ttcgtgaaaa atacacgcgc tgttaccact ttaattttta
    21721 ttctttataa tcttttagaa taactttgaa cgttgagcct ttgccaactt gactattcac
    21781 ttcaatattg ccttggtggg cctctactat atgctttgta attgataatc caagacctgt
    21841 cccaccagaa tctctacttc gtgctttatc tactctataa aatctttcaa aaatacgttg
    21901 ttggtcttct aatttaatac ctataccaaa atcttgtact tcgaaaatga cacgaaagtc
    21961 atctcgatac acacgaacgt taatatctcc atcttcataa gaatagttaa ttgcattcgt
    22021 taacaaattc gtgataactt gagcaatttt actttcttgt gctttaacaa tgacatcttt
    22081 ttcaatatca gtatgaatgg aaatattttt ttgattggct tgagtcatca tattatcaat
    22141 aatacgccgc gttaaatctg ataaattcat atagtctgta tctaactctg tatgttgctc
    22201 aatatgcgat aaatctaaca gatctgttac taaagattct attcgatttg attcttttaa
    22261 aattatgttt aaaaacatgt ctaatgattc cgcatcattt tttgcaccat caataagcgt
    22321 ttcagcaaac cccttaattg aagtaatagg tgtttttaat tcatgtgaaa catttgctac
    22381 aaattcacgt cttagatttt caagttgttt cagatttgtt atatcatgca tcacaactaa
    22441 aatcccttgc aaactttttt gagacctagt taaaatcgga acgcatgaaa tatcaaagta
    22501 cttggcatgg acttggttta ttgcaacttc caattgttca taaataggtt tttcaacttt
    22561 aaaactttct aaaattaatt gctcaatttc agtattaaca tagccgtgat agcctacttg
    22621 ttcaatatta tgcgagatgt tgaactgttc ataatacgct ttatttgcaa caacgatttt
    22681 tccatttcga tctatcatta aaatagcact tggaatattt tcaatcgttg tttttaaacg
    22741 gttggattga attttttgct cattattaag cttttgaagg cgtcgtgcta aatcattggt
    22801 agacacaaaa agcgctttag tttctacaac attactttca ggtacacgta tgtgataata
    22861 accatttgcc aacaattgtg ttgcataagt aacttcttga atgggacgga ttaatgtacg
    22921 cttaaaacta cggcttgcaa aatacagaca aatgagtaca actaaacatg tcaaaataag
    22981 atatttccac aacgtccaat gcatttctgt aatatcgtta ttgtaacctt taatccatac
    23041 atgataaccg ttaaccttct tattaaaaat aaaaacgtcc cttttttgaa tatagctatc
    23101 actatttggg atagctttca gtttgccact actaaacacc tttttatcat gcttagttat
    23161 caataaatct atattttgct gttttacaat ttttttaact ttatcaatct cattatcttg
    23221 gactaaataa atatatgatc ttgcatctgt tgctagagct tgttcgtgtt tttctgataa
    23281 aacatatgtg atggaagcgt gaataataat gcctaatgta acaaaactga taattaatat
    23341 actgcttatc aataacatta agcggtggtg aaacttcatc attgttcttt aggtctttcc
    23401 aatttatagc ctaagccacg cacagtttta ataagttgtg gcttcttagg attatcttct
    23461 aatttatctc ttaaatgact gatatgtaca tcaacaattc ttgagtctcc tgcaaattca
    23521 taattccata ccgtatttaa catatgctct ctcgtaatga ctctgccttg tctttctatc
    23581 aaataaagca agagttcaaa ttctttcggt gttagctcca ataactcatt atgcttatat
    23641 acttcaaaat aatcgggtct aatacgtatc gatccgatgg taatatcatc gtcaatctct
    23701 tctacttctt ttacaaattg agaacgtctt aaaatggctt tcacacgggc aacaacttct
    23761 ctaggtgaaa aaggcttagt catatagtca tcggcaccta attctaaacc taatacccga
    23821 tcaaattcat catttttcgc cgttaacatt aatataggga ctaaattttt atttgttctt
    23881 acagtcttac atacgtcaat gccatctttt ttaggtagca taacatctaa aataattaaa
    23941 tctggctgtt cactttctac cttttctaaa gcctcatcac catcaaatgc gacaacaact
    24001 tcataaccag ctgtttctaa gttatattta agtaatgtta cgattgaatg ttcgtcatct
    24061 actaccaaca ctttttgcga catggtatgc ctccctaact tataattata tttacattat
    24121 aaccgaacta tttataaaaa taacatccta cacattatct ttacacattt tttacattac
    24181 tttacatata aataaaatac ttcttatatt tccttctatc atttgcatga cttactctgg
    24241 gacaacgaaa taaattttgt aaaaatagca tttctatccc actacctatg catgagtttt
    24301 actcatttat tcctaagctt atgtacatat tcgctttgtc taatgtgtaa gaaacactac
    24361 ataatcaatc attggtgact ctttattatt tctatcctgt tgccaacttc aattcattta
    24421 aaaaggcgaa cctagcaatt aaagttgcta gactcaccta attttatttt gcttatttta
    24481 aatttttaat caattcatct gcaaatgctg atgtagaaac ttcttcagca ccatccatta
    24541 aacgggcaaa gtcataagta acaacttttg aagcaattgt atcttcaatt gaatctgtaa
    24601 tcttatctgc cgcttcttgc catcctaaat gttctaacat taatacagaa cttaaaatta
    24661 ctgaagatgg attcacttta tttaaacctg catattttgg agctgtacca tgtgttgctt
    24721 caaaaatagc atgacctgtt tcataattaa tgtttgcacc tggcgcaata ccaataccac
    24781 caacttgtgc agctaaagca tctgaaatat agtcaccatt caagttcata gttgctacaa
    24841 catcatgctc agctggacga gttaaaattt gttgtaagaa aatgtcagca atagaatctt
    24901 taatgataat cttgccttct ttttcagctt tttcttgagc agcattagca gcatctctgc
    24961 cttcattttc aacaatttcg tcatattgtt gccaagtgaa tacttgatca ccaaattcag
    25021 ataatgctaa atcgtaaccc cactgcttaa atgagccttc tgtaaatttc ataatattac
    25081 ctttatgaac taaagtaact gatttacggt tattatcgat agcatattgt atagctgctc
    25141 taactaatcg ctcagttcct tctttagaaa ctggtttaat accaatacct gaagtttctg
    25201 ggaatcgaat gtttgtcgca cccatttcgt tttgtaagaa gtcaattacc tttttaactt
    25261 ctgttgtacc ttctttaaat tcaataccag cataaatgtc ttcagtattt tcacggaaaa
    25321 taaccatatc aacatcttgt ggacgtttaa caggtgatgg tactccttta aaccaacgta
    25381 ccggtcttaa gcaagtaaat aaatctaatt cttggcgtaa agccacattt aatgatctaa
    25441 taccaccacc aattggtgtt gttaaaggtc ctttaacagc aattaaatat tctttaattg
    25501 tatcaagtgt ttcttgaggt aaccattcac cagttgtatc aaatgctttt tggccagcta
    25561 gcacttcttt ccattcaatg cgtttttcgc cattataggc tttctcaaca gcagcatcta
    25621 taactcggct tgctgccttc caaatatccg gtccaattcc atcaccgata ataaatggga
    25681 taattggttc attaggtacg tttaatcctt cagttccttg agtaattttt tctgcagtca
    25741 tagttatttt acctccaaat tttacatctt catttttaat ttgtattgat tattttcttt
    25801 cttcaagcgg gatatactta cgattcgttt cgccaatata tttcgctcta ggacgcataa
    25861 ttctattatc tttatattgt tctaaaatat gagcaatcca tcctgcagaa cgacttacag
    25921 caaagattgg cgtgaataag tcatgaggta tttccataca gtgataaaca ctcgcactat
    25981 aaaaatcaac attaggaatt aatccttttt cttctgccat acgtttttcc attttcactg
    26041 acatttcaaa taattcttca cgaccagcgt ctttcgtaat ttgacggctc atttctctta
    26101 aatatttcgc tctaggatca ccatctttat atacacgatg accgaagccc attactttat
    26161 ctttattagc aaatttttca tctaagtaag catcaacatt ttcaattgac ccaatctcag
    26221 ataacatcgt cataacttgt tcgtttgcac caccatgtaa tggccctttc agagaaccta
    26281 cggctgctac aatacctgag tacatatctg acaatgatga taccgcacaa cgtgccgtaa
    26341 atgcagatgc gttcaactca tgatcagcgt gtaaaataag tgctttattg aaggcttcta
    26401 cttctatatc tgttggtaat tccccacgta acatatatag gaagtttgcc gcataactta
    26461 agtcaggatt aggcttaagt ggttctttat cttgtcttac tcgagcaaac gctgtaacta
    26521 atgatgctac tttagcctgt atacgcattg ctctttcata acgattttca tctgattcat
    26581 tttcagcatc aggatcgaaa tgtgcaatat atgataatga cgtacgtaat gctgtcattg
    26641 gatgcacgtg atctgtaaca tactcctcaa aatgtgtata cacacgagga tttaatgtca
    26701 tgtattgatt taatttccct tttagatgag caagctcttc ttcgtttggc aatctatagt
    26761 tccataatag gaaaataact tcttcaaatt gcgcattttc agctagatca tcaatatcat
    26821 agccggcata agtcaattga ctttcaataa ttgaacttat tttagtctcc gctgcgataa
    26881 ccccttctaa acctctttgt aattctgcca tgataaattt cccctttact gtttctttat
    26941 gaaatggctt tcaactaata ttatagctaa acgcatttcg tttgtgaaat ataaaaactg
    27001 gcattatatg tgcaacaatt tttttgtaaa tctatgtaaa tgtcacagaa tagccacaaa
    27061 tagcaccatt aaagcttttt caagatgtga ataatccatg aattttacaa tagaattaaa
    27121 ttttcaattg gttattatat atgattataa aaaccaaagt atattaacat ggaattttac
    27181 acatttttac cgaaaataat tgtatatatc tcaatataag tgcataataa acatatctat
    27241 atttaaggga gatgtattat ggctgaaaaa ttacaaaggg aactgagcaa tcgccacata
    27301 caattaattg caattggcgg tgcaattggt acaggcttat tcttaggtgc tggtcaaacg
    27361 attgcattaa ccggcccttc aattctatta acatacatca ttataggatt tatgttattt
    27421 atgtttatgc gaggtttagg agaaatcatt atacagaata ctgaatttaa atctttcgca
    27481 gatgtaacca atacatatat tgggcctttt gcaggatttg ttaccggatg gacatactgg
    27541 ttctgttgga ttattacagg tatggctgaa gtaacggctg tggcaaaata tgttagcttt
    27601 tggttcccag aaattccaaa ctggataagt gcactatttt gtgtactgtt attaatgtca
    27661 ttcaacctac ttagcgcaag acttttcgga gaattagaat tttggttctc tatcattaaa
    27721 atagcgacaa ttattggttt aatagtagtt ggtttcgtca tgattctatt tgcatttaaa
    27781 actcaattcg ggcatgccag tttcacaaat ctatatgaac acggcatatt cgctaaaggt
    27841 gcttctggat tctttatgtc tttccaaatg gcactattct catttgtagg aattgaaatg
    27901 attggtgtta cagctgggga aacaaaagat ccagttaaaa caattccaaa agcaattaac
    27961 agtgtaccca ttagaatttt aatattttac gttggggcgt tagcggttat catgtctatt
    28021 atcccttggc agcaagttga tcctgataac agtccattcg taaaattatt cgcattgatc
    28081 ggaattccgt ttgctgcggg cttgattaat tttgtagtat taaccgctgc tgcttcatca
    28141 tgtaacagtg gtatattctc aaatagccgt atgcttttcg gtttatcaag tcaacaacaa
    28201 gcacctccga acttttctaa gacgaataaa tatggcgttc cacatgttgc aatctttgct
    28261 tcatcagcat tattacttgt ggcagcatta ctaaactata ttttcccaga tgcgacaaaa
    28321 gtatttacgt atgtgactac catctctaca gtgttatttt tagttgtatg gggtctgatt
    28381 atcattgcat atatcaatta tagtcgtaaa aacccagatc tacataaaaa tgctacgtac
    28441 aaactattag gtggcaaata tatgggctac ttaatatttg tattcttcat ttttgtgttc
    28501 gggttattat ttattaatgt tgatacaaga cgtgcaattt attttattcc gatttggttt
    28561 atacttttag catttatgta cttaagatat aaacgtatcg ctgctaaatc aaataaataa
    28621 caacaagttt tagggcttgg gacattaagt tcttaggcaa tgtaaaaaag ctgatttcta
    28681 ttaattattt gatagaaatc agcttttttg atatgtattt tataatgtac agctcgttga
    28741 gctgctattt tccttatatt aagtgccatc aatacaaaac ctagctctcg tttaacttta
    28801 tttattcctc gaactgacat tcgagtgaaa cccaaaatag ccttcataaa tccaaaagca
    28861 ggctctacat caatttttct ttgactatag at
//
LOCUS       DALAGD010000027        28615 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.027, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000027 DALAGD010000000
VERSION     DALAGD010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 28615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28615
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.027"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            86..829
                     /gene="sarU"
                     /locus_tag="O2701_000945"
     CDS             86..829
                     /gene="sarU"
                     /locus_tag="O2701_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarU"
                     /protein_id="HCZ4292850.1"
                     /translation="MDYQTFEKVNKFINVEAYIFFLTQELKQQYKLSLKELLILAYFY
                     YKNEHSISLKEIIGDILYKQSDVVKNIKSLSKKGFINKSRNEADERRIFVSVTPIQRK
                     KIACVINELDKIIKGFNKERDYIKYQWAPKYSKEFFILFMNIMYSKDFLKYRFNLTFL
                     DLSILYVISSRKNEILNLKDLFESIRFMYPQIVRSVNRLNNKGMLIKERSHADERIVL
                     IKINKIQYNTIKSIFTDTSKILKPRKFFF"
     gene            complement(1040..1906)
                     /gene="galU"
                     /locus_tag="O2701_000946"
     CDS             complement(1040..1906)
                     /gene="galU"
                     /locus_tag="O2701_000946"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCZ4292851.1"
                     /translation="MKKIKKAIIPAAGLGTRFLPATKAMPKEMLPILDKPTIQYIVEE
                     AARAGIEDIIIVTGRHKRAIEDHFDSQKELEMVLKEKGKSELLEKVQYSTELANIFYV
                     RQKEQKGLGHAISSARQFIGNEPFAVLLGDDIVESEVPAVKQLIDVYEETGHSVIGVQ
                     EVPEADTHRYGIIDPLTKNGRQYEVKKFVEKPAQGTAPSNLAIMGRYVLTPEIFDYLK
                     TQKEGAGNEIQLTDAIERMNNDNQVYAYDFEGERYDVGEKLGFVKTTIEYALKDDSMR
                     EELTRFIKALGL"
     gene            complement(2086..4908)
                     /gene="fnbB"
                     /locus_tag="O2701_000947"
     CDS             complement(2086..4908)
                     /gene="fnbB"
                     /locus_tag="O2701_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbB"
                     /protein_id="HCZ4292852.1"
                     /translation="MKSNLRYGIRKHKLGAASVFLGTMIVVGMGQEKEAAASEQNNTT
                     VEESGSSATESKASETQTTTNNVNTIDETQSYSATSTEQPSQSTQVTTEEAPKTVQAP
                     KVETSRVDLPSEKVADKETTGTQVDIAQPSNVSEIKPRMKRSTDVTAVAEKEVVEETK
                     ATGTDVTNKVEVEEGSEIVGHKQDTNVVNPHNAERVTLKYKWKFGEGIKAGDYFDFTL
                     SDNVETHGISTLRKVPEIKSTDGQVMATGEIIGERKVRYTFKEYVQEKKDLTAELSLN
                     LFIDPTTVTQKGNQNVEVKLGETTVSKIFNIQYLGGVRDNWGVTANGRIDTLNKVDGK
                     FSHFAYMKPNNQSLSSVTVTGQVTKGNKPGVNNPTVKVYKHIGSDDLAESVYAKLDDV
                     SKFEDVTDNMSLDFDTNGGYSLNFNNLDQSKNYVIKYEGYYDSNASNLEFQTHLFGYY
                     NYYYTSNLTWKNGVAFYSNNAQGDGKDKLKEPIIEHSTPIELEFKSEPPVEKHELTGT
                     IEESNDSKPIDFEYHTAVEGAEGHAEGTIETEEDSIHVDFEESTHENSKHHADVVEYE
                     EDTNPGGGQVTTESNLVEFDEDSTKGIVTGAVSDHTTIEDTKEYTTESNLIELVDELP
                     EEHGQAQGPIEEITENNHHISHSGLGTENGHGNYGVIEEIEENSHVDIKSELGYEGGQ
                     NSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNNGNQSFEEDTEKDKPKYEQ
                     GGNIIDIDFDSVPHIHGFNKHTEIIEEDTNKDKPNYQFGGHNSVDFEEDTLPQVSGHN
                     EGQQTIEEDTTPPIVPPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEVPTEPGK
                     PIPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVVPTKKAQSKKSELPETGGEESTN
                     NGMLFGGLFSILGLALLRRNKKNHKA"
     gene            complement(5590..8646)
                     /gene="fnbA"
                     /locus_tag="O2701_000948"
     CDS             complement(5590..8646)
                     /gene="fnbA"
                     /locus_tag="O2701_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbA"
                     /protein_id="HCZ4292853.1"
                     /translation="MKNNLRYGIRKHKLGAASVFLGTMIVVGMGQDKEAAASEQKTTT
                     VEENGNSATDNKTSETQTTATNVNHIEETQSYNATVTEQPSNATQVTTEEAPKAVQAP
                     QTAQPANIETVKEEVVKEEAKPQVKETTQSQDNSGDQRQVDLTPKKATQNQVAETQVE
                     VAQPRTASESKPRVTRSADVAEAKEASNAKVETGTDVTSKVTVEIGSIEGHNNTNKVE
                     PHAGQRAVLKYKLKFENGLHQGDYFDFTLSNNVNTHGVSTARKVPEIKNGSVVMATGE
                     VLEGGKIRYTFTNDIEDKVDVTAELEINLFIDPKTVQTNGNQTITSTLNEEQTSKELD
                     VKYKDGIGNYYANLNGSIETFNKANNRFSHVAFIKPNNGKTTSVTVTGTLMKGSNQNG
                     NQPKVRIFEYLGNNEDIAKSVYANTTDTSKFKEVTSNMSGNLNLQNNGSYSLNIENLD
                     KTYVVHYDGEYLNGTDEVDFRTQMVGHPEQLYKYYYDRGYTLTWDNGLVLYSNKANGN
                     GKNGPIIQNNKFEYKEDTIKETLTGQYDKNLVTTVEEEYDSSTLDIDYHTAIDGGGGY
                     VDGYIETIEETDSSAIDIDYHTAVDSEAGHVGGYTESSEESNPIDFEESTHENSKHHA
                     DVVEYEEDTNPGGGQVTTESNLVEFDEESTKGIVTGAVSDHTTVEDTKEYTTESNLIE
                     LVDELPEEHGQAQGPVEEITENNHHISHSGLGTENGHGNYDVIEEIEENSHVDIKSEL
                     GYEGGQNSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNKGNQSFEEDTEKD
                     KPKYEHGGNIIDIDFDSVPHIHGFNKHTEIIEEDTNKDKPSYQFGGHNSVDFEEDTLP
                     KVSGQNEGQQTIEEDTTPPIVPPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEV
                     PSEPETPTPPTPEVPAEPGKPVPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVAPT
                     KKPQSKKSELPETGGEESTNKGMLFGGLFSILGLALLRRNKKNHKA"
     gene            complement(8630..8731)
                     /locus_tag="O2701_000949"
     CDS             complement(8630..8731)
                     /locus_tag="O2701_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292854.1"
                     /translation="MLLKTEQYRLAFIKFKKLMNFAFKGRYYSEKQS"
     gene            complement(9115..10473)
                     /locus_tag="O2701_000950"
     CDS             complement(9115..10473)
                     /locus_tag="O2701_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease"
                     /protein_id="HCZ4292855.1"
                     /translation="MFNEIWPLISVVLGIVILLVLIIGFKLNTFISLIITSMITALML
                     GIPLTKIMETIEKGMGSTLGHIALIFGLGAILGKLLADGGGATRIADTLIQKFGQKHV
                     QWAMLVAAFIVGIALFFEVGLVLLIPLVFTVAKRANVSVLKLGLPMVTALSVTHGFLP
                     PHPGPVVIAKELKANVGDVLLYGMIIAIPVTLIAGPIFNKVAQKMIPSAYTREGDISA
                     LGAQKEFTDQEMPGFGMSLLTATLPVILMLVSTITQLVTGHDKPTNLFESIIYMIGTA
                     GTAMLIAVLFAIVTMGLMRKRKMNHIMESVTNAIYPIGMMLLIIGGGGTFKQVLIDGG
                     VGNTIAKMFEGTEMSPILLAWIVAAVLRIALGSATVAAISTTGIVLPLLQSSDVNVAL
                     VVLAIGAGSVILSHVNDAGFWMFKEYFGLTVKETFLTWSLLETIISVSGIIFILFISL
                     FV"
     gene            complement(10590..12143)
                     /gene="gntK"
                     /locus_tag="O2701_000951"
     CDS             complement(10590..12143)
                     /gene="gntK"
                     /locus_tag="O2701_000951"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCZ4292856.1"
                     /translation="MKYMIGVDIGTTSTKSVLYDENGTFIMKHQIGYDLHTPNVDVSE
                     ENPDELFDAVLMTIKYVMRESKVNKDDIKFVSFSAQMHSLIAMDQQHQRLTNNITWAD
                     NRAAKYATVINEVHDGNAIYQRTGTPIHPMSPLAKIFWMKHEWQDVFQRTAKFADIKT
                     YIFYHLFDTYIIDYSMASATGMFNLETLDWDVEALELLGISKEMLPELVPTTYVMKGM
                     KERYATLMGLNKDTPFVIGASDGVLSNLGVNSVGKGEVAVTIGTSGAIRTVIDKPRTD
                     YKGRIFCYVLTEDHYVIGGPVNNGGVVLRWLRDELLASEVETAKRLGVDPYDVLTQIA
                     KRVKPGADGLIFHPYLAGERAPLWNANARGSFFGLTLSHKKEHMIRAALEGVLYNLYT
                     VYLALIEVMNETPKMIKATGGFAKSEVWRQMMSDIFDTELVVPESYESSCLGACVLGL
                     KAVGDIEDFSIVSSMVGATNNHTPIEENVTVYQEIVSIFINLSRSLTENYEQIADFQR
                     QHIAENKTQ"
     gene            complement(12167..12847)
                     /locus_tag="O2701_000952"
     CDS             complement(12167..12847)
                     /locus_tag="O2701_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCZ4292857.1"
                     /translation="MMYGYPEKWLEGMTTGEGIAAELRLGIVNGHIAEGTLLTENQMA
                     KQFNVSRSPIRDAFKLLQQNQLIQLERMGAHVLPFGEQEKKEMYDLRLMLESFAFSRV
                     KNQERLPIVKEMKKQLEMMKVAVKFEDAESFTKHDFEFHETLIKASNHQYLNSFWSHL
                     KPVMMALVLTSMRQRMQQNPQDFERIHHNHQVFIDAVEQYDSQILKEAFHLNFDDVGK
                     DIEGFWLN"
     gene            complement(13001..13765)
                     /locus_tag="O2701_000953"
     CDS             complement(13001..13765)
                     /locus_tag="O2701_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCZ4292858.1"
                     /translation="MSNYSTGELAKLCNVTTRTIQYYDRKGILKPQGFTEGKRRVYTE
                     QQRQTLELILLLKDLGCALSDIDMLLKGEGTLKTLNTLLTMKQQEINQQVKQQQAVLN
                     KIKNVQYYVNEASTSPITHLKDIEHVMSKSAEMKSIRRNIWISAGIIGIIQYSSIISA
                     ILMKNKWPFLIALPFMIGYGIGVTFYYQQKVAYLCPNCQHIFSPSLWAVIKAKHTATT
                     RRFECPNCHETHYCIEVPKAHMSTEQLEISHIQHNN"
     gene            complement(13930..14622)
                     /locus_tag="O2701_000954"
     CDS             complement(13930..14622)
                     /locus_tag="O2701_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCZ4292859.1"
                     /translation="MYVDRKPSLYLEDLRHDFKNSLSKFENGDEAFDTLLGFVELDHI
                     YSSALKEISTKLSILDDNFNHIYKHNPIHHMERRVKEMRSLIEKLNRKGLQISAETAK
                     EHILDIAGIRVVCNYLDDIYLIEEMLLKQEDVQLIKRKDYIQHPKENGYRSLHIVVSI
                     PVFLAERVEVLPVEIQIRTIGMDMWASLEHKIRYKNNAETEKYRDLLKECATEITEVE
                     DKLQQIHSEITE"
     gene            complement(14853..15269)
                     /locus_tag="O2701_000955"
     CDS             complement(14853..15269)
                     /locus_tag="O2701_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HCZ4292860.1"
                     /translation="MPFTMKGYPEKWKEFEVIKRQKAIEIANAMLKEGYLEKDVVPIA
                     AKKAKDWYRALSKEEIKALEEENFVQFHTATMEKTEVDSEYATTDNEEHIQEQSNQDK
                     SYFDSESIGNQSGGVRQREPLMDGPHPKVVESFKDV"
     gene            complement(15408..17231)
                     /locus_tag="O2701_000956"
     CDS             complement(15408..17231)
                     /locus_tag="O2701_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCZ4292861.1"
                     /translation="MIIPAIIGYLCGNFQFGLLVATGTLAHIYVFKGPSRSKLRTVII
                     CNLAFAICMMLGTLTAKTPLVFGMTLLIVTVIPFYIFTALKIAGPSSTFFIVTFSLPI
                     NLPIAPEEALYRGFAILVGGILATMMVLITIVFSKNKAEEQAIQNDFKLISKLLHTYN
                     DKSAFLQVAKTAVDSFKASDKLLITSTSSNDKLSRRFQKLLLLHTSAQGIYSELLELN
                     AKQIRPLPDELIEMMDHIIAQLDNSEENVRYWRKEVTVTEEFQNLFNHILKIDEMVHA
                     NEARIAYEADMRKPLYSKRIYQNLTLDSIVFRNTLRYTAIMMIAIFIALMFDFEKAYW
                     IPLSAHTILLGTSTIHAIERGMARGLGTILGVLVLSVILLFSIPTPVAVILMGIAALF
                     TEALVGANYAIAVVFITIQVILMNGLASQNLTINIAFPRVIDVAMGIVIAIIGLFVLG
                     QRTASALLPNVMAEVVRKEATLFHYLFSENQYKDDVYQKNTAMNLSVKLNNMTQVYNA
                     ANGELFSDKTLIQNYYPSLFALEEISFMLNRAMANEDRLTINEQLMGEYLAIFENIAK
                     HFELNTELEIKILPDLPQYNYIQSAMMNIQHNGFRERDKNV"
     gene            complement(17709..18986)
                     /locus_tag="O2701_000957"
     CDS             complement(17709..18986)
                     /locus_tag="O2701_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ4292862.1"
                     /translation="MKEKRTNVRWMFALAFFFIGVIAYMDRANISYIAKQMMDDLGMT
                     KPQFGLLASFFSLGYALMQVPSGMLAEKFGPRKMITIALVWWSAFTILTGMIKNHGLI
                     YLVRFLFGVGEAPMYPSNAVFNSFWFSKNEKGRASSALLAGSYFGPVLAPIVTIAIVN
                     AFNWQAVFYIFGAVGILMAVLWAIIAKDLPEQHRMVNEAEKRFIMENRDIVATEKSSP
                     PWNDFFKRFSFYAIAIQYFVVQFIITLFLIWLPTYLTEVFHVNFKEMSISSLPWLLMF
                     FLILSAGAISDRVLGLGRSKFVARGVIAIAGFIVFAVSIIFAVRTGNLYVSIFWLSLG
                     LGGIGISMGMSWAAATDLGRNFSGTVSGWMNLWGNIGALISPLLAGLFVEHLGWTMTF
                     QLLIVPAVIAVIMWFYVKPDQPLIVSDDKAIEK"
     gene            19533..20144
                     /locus_tag="O2701_000958"
     CDS             19533..20144
                     /locus_tag="O2701_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCZ4292863.1"
                     /translation="MEQIITEFISRFGYAAIFILILLENVLPIVPSEIILTFAGLMSV
                     KSHLSILTLFIIATIASFIGLLILYYICRLISEERLYRFIDRHGKWIKLKSKDLKRAN
                     DWFKKYGVWAVFICRFIPVLRVLITIPAGVNRMNVVTFTVISLIGTTIWNFGLILLGR
                     TLSDSFGMLMTGLHTYSRIMYVVIIIAVIYFAIRYIGKRKRVK"
     gene            complement(20153..20251)
                     /locus_tag="O2701_000959"
     CDS             complement(20153..20251)
                     /locus_tag="O2701_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292864.1"
                     /translation="MPNKLKKAVNHFNRWNDSLLFVYRNLKFMPLC"
     gene            complement(20241..21404)
                     /locus_tag="O2701_000960"
     CDS             complement(20241..21404)
                     /locus_tag="O2701_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ4292865.1"
                     /translation="MINSNIRLYILGFLAFFASLIQNIYTPIIPRLYDDFQVPLFWIN
                     ATVGGFIFIVAVMQIVLGRSIDSRDSKKVLLTGLGIVIISSFICAVTHNFILFAISRL
                     FQAIGCGIIPLVTLTLLAKLSTDNGRAQAMANYQIFLSCAPALAPILGSTLGARWDYI
                     GIFSFLLVISIVLFLIIFFIDIPNVEKGIVKLTEKIEEKYLTDKVFITLVTLGFLIFF
                     TYFSILVYLPTLLNNTYDIGVGISGVLFLPITVSVILGSLFYKRFSKKYNEIMILRVT
                     ITGFAIFTLLFGWLNESNVIILSVIIFILGTCVGIVPALLSTIISKRFEHIKGKVLGV
                     FNFVRYIGMTVGALLIGIISQPLVAFYFTTITIMLIVIFLYIKIVDFQLKYAK"
     gene            complement(21397..21855)
                     /locus_tag="O2701_000961"
     CDS             complement(21397..21855)
                     /locus_tag="O2701_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCZ4292866.1"
                     /translation="MKREVILKLQQFIIERENADKRRQYKKENEELDLSLTQFHIIEL
                     IDNNDKVNNKFLSEMLNVSKPAITKSIKKLLAKDLVVESHNEFNKREVNYSLTQKGKK
                     LSYIHDELHEKSVKKYEEVLKVFDDDEMAVIIEFLNRSIEELKKEDGLND"
     gene            22289..22984
                     /locus_tag="O2701_000962"
     CDS             22289..22984
                     /locus_tag="O2701_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCZ4292867.1"
                     /translation="MDVLTIEHLTKKIGNKTILEDVSFKLKRGQIVGLVGANGAGKTT
                     LMKVILGYSSFQSGNFNVINSKDSKSNIGALIENPGIYPFMSGYENLKLLNESKNTQD
                     IDKIVSQLHMDEYIHKKAKTYSLGMKQKLGIAIAFLNKPQFIILDEPMNGLDPKAVRD
                     VRELIVQKAQEGVTFLISSHILSELVKITNSILIINKGKIVTETSEEELKQFKDNDLE
                     NVLLEIIEREDQA"
     gene            22986..23756
                     /locus_tag="O2701_000963"
     CDS             22986..23756
                     /locus_tag="O2701_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4292868.1"
                     /translation="MGTLIKQECFKLFKKKSTFIAPIVFILLMVAQGYIATKYNEIFT
                     PQESFTSAYNGFSWFAFLLIIQASTIISMEFHYGTIKNLLYREYSRTTMIVSKIITLF
                     IISLIYFVITIIASIVIGSLFFNDLNIFESSGNQLSLLNQLLLVSLGTFVGVWLVLSL
                     TLLLSSATNSTGVAIAVGIVFYFASSILAVIQTALLEKIDWLKWNPINMMNIMLQTVE
                     KGFSKSTKLELHELFIGNIAYISIFLILVVFIFKKKNI"
     gene            24129..26093
                     /locus_tag="O2701_000964"
     CDS             24129..26093
                     /locus_tag="O2701_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HCZ4292869.1"
                     /translation="MTQITEKELKKKYLDLLSQNFDTPEKLATEIINLESILELPKGT
                     EHFVSDLHGEYEAFQHVLRNGSGNVRAKINDIFKERLSTKELNDLTALVYYPEDKLKL
                     IKSDFQNCGQLNVWYITTIEHLIELIKYCSSKYTRSKLRKALPKQYVYIIEELLYKSN
                     EYQNKKSYYETLVNQVIELKQADDLIIGLAYSVQRLVVDHLHVVGDIYDRGPQPDKIM
                     DTLINYHSLDIQWGNHDVLWVGAYAGSKVCLANLLRICARYDNLDIIEDAYGINLRPL
                     LTLAEKYYDADNPAFKPKKRPDKHERLTQREESQITKIHQAIAMIQFKLEIPIIKRRP
                     NFEMEERLVLEKVNYDTNEITVYGNTYPLKDTCFQTVNRDNPAELLPEEEEVMNKLLL
                     SFQQSEKLRRHMSFLMRKGSLYLPYNGNLLIHGCIPVDENGEMESFEIDGHTYSGQEL
                     LDVFEYHVRKSFDEKENTDDLSTDLVWYLWTGKYSSLFGKRAMTTFERYFIADKASHK
                     EEKNPYYHLREDVNMVRKMLSDFGLNPDEGRIINGHTPVKEINGEDPIKADGKMLVID
                     GGFSKAYQSTTGIAGYTLLYNSFGMQLVAHQQFNAKEKILSEGIDELSIKRVVDKELQ
                     RKKIRDTNIGKDLQAQIDILKMLMHDRYLD"
     gene            26437..27516
                     /locus_tag="O2701_000965"
     CDS             26437..27516
                     /locus_tag="O2701_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292870.1"
                     /translation="MRILKESIIVAFAFVGVVVGAGFATGQEIFQFFTSHGAYSISGI
                     IVTGLLITLGGMVVMHTGHHLKSRNHSDSINYFLYPSIARGFDIILTMFMLSLAIIMT
                     AGGASTIHQSFNLPYWLSALILVAFILATLFLKFDRLIAVLGGVTPFLIAIVIMIAVY
                     YFTTSHLDFTAANNDAQIHKQKSLSPGWWFDAINYASLQIAAAFSFLSVMGSKVKYRD
                     STLYGGLIGGLIITFLLMMINLGLISQFDKIKHVDLPTLKLATQMSPSIGIIMSVIMI
                     LVIYNTVVGLMYAFASRFSVPFSRRYFIIIITMAVITYISTFIGFISLIGKVFPIMGL
                     FGFILLIPVLYKGLIKRITGKSHID"
     gene            complement(27621..28214)
                     /locus_tag="O2701_000966"
     CDS             complement(27621..28214)
                     /locus_tag="O2701_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCZ4292871.1"
                     /translation="MEHIFREGQNGAPTLILLHGTGGDEFDLLPLGEALNENYHLLSI
                     RGQVSENGMNRYFKRLGEGVYDEEDLAFRGQELLTFIKEAAERYDFDIEKAVLVGFSN
                     GSNIAINLMLRSEAPFKKALLYAPLYPVEVTSTKDLSDVSVLLSMGKHDPIVPLAASE
                     QVINLFNTRGAQVEEVWVKGHEITETGLTAGQQILGK"
     gene            complement(28236..>28615)
                     /locus_tag="O2701_000967"
     CDS             complement(28236..>28615)
                     /locus_tag="O2701_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCZ4292872.1"
                     /translation="VGEGGNGGQVILIKDDKGPAARQGYGEVHHVSFRVKDHDAIEAW
                     ATKYKEVGINNSGIVNRFYFEALYARVGHILIEISTDGPGFMEDEPYETLGEGLSLPP
                     FLENKREYIESEVRPFNTKRQHG"
BASE COUNT         8838 a         5301 c         4176 g        10300 t
ORIGIN      
        1 aacatgtctt taaaagagat tgcttttata ttactttaaa taatataaag tattgggaca
       61 tcagaaaata gtaggagaat gtaaggtgga ctatcaaact ttcgaaaagg tcaataaatt
      121 cataaatgta gaagcgtaca tattttttct cactcaagag ttaaagcaac aatataaatt
      181 atcattaaaa gaattgttga tattagcata tttttattac aaaaatgaac acagtatttc
      241 actaaaagaa atcattggtg acatacttta caaacaatct gatgttgtaa agaacattaa
      301 gtcactatct aaaaaaggat ttataaataa gtctagaaac gaagcagatg aacgccgtat
      361 ttttgtttca gttactccaa tacaacgtaa aaagattgct tgtgttatta atgagttaga
      421 taaaataatt aaaggattta ataaggaaag agactacata aaatatcaat gggctccaaa
      481 atatagcaaa gaatttttta tactttttat gaacattatg tactcaaaag attttttaaa
      541 atatcgattt aatttaacat ttcttgattt atctatctta tatgtaatat catctcgaaa
      601 aaatgagata ctaaatttaa aagatttgtt tgaaagtatt agatttatgt atcctcaaat
      661 tgttaggtca gttaatagat taaataataa aggtatgcta atcaaagaac gatcccatgc
      721 agatgaaagg attgtgttaa tcaaaataaa taaaatacaa tataacacta ttaaaagcat
      781 attcacagat acttccaaga ttctcaaacc aagaaaattt ttcttttaaa tttaaacaga
      841 tttacctctt gataaaataa ataagcaatc atactacttc tcaatttagt ataaataaaa
      901 atacataatt aactttcttt tgtttttata ttatttcaat accctactat atatcacaac
      961 acataaatta agcatgacac tcattcaatt tagttcacca tttcgtgttc caattttact
     1021 gagtatcatg cttttaatgt tataaaccta atgctttaat aaatcgtgtt aattcttctc
     1081 gcatactgtc atctttcaat gcatattcta tggtagtttt aacgaagcct aatttttctc
     1141 caacgtcata acgttcgcct tcgaagtcat atgcatacac ttggttatca ttattcatac
     1201 gttcaatcgc atctgttaac tgaatttcgt tacctgcgcc ttctttttgc gtttttaaat
     1261 aatcgaaaat ttcaggcgtt aatacataac gtcccataat agctaggttt gatggtgccg
     1321 taccttgtgc tggcttttca acaaactttt tcacttcata ctgacgtccg tttttagtta
     1381 atgggtcaat aattccataa cgatgagtat ctgcttccgg aacttcttgg acacctataa
     1441 ctgagtgccc tgtttcttca taaacgtcaa tcaactgttt cactgctggc acttcagatt
     1501 caacaatatc gtcacctaat aataccgcaa atggttcatt gccgataaat tgacgcgcag
     1561 aactaatcgc atgccctaaa cctttttgtt ctttctgcct tacataaaaa atattcgcaa
     1621 gttccgttga atactgaact ttctctagta attcagattt acctttttct tttaacacca
     1681 tttctaattc tttttgacta tcaaaatgat cttcaatcgc gcgtttgtgg cgacctgtca
     1741 ctataataat atcttcaatt ccagctcttg cagcttcttc aacgatatat tgtattgtgg
     1801 gtttatctaa gataggaagc atttcctttg gcatcgcttt agttgctggt aaaaatctag
     1861 tccctaaacc agcagcggga atgattgcct tttttatttt tttcaaagtt aatgtgctcc
     1921 ttttcctaag tattaaatct atgtatcaac gtcattttaa cactaattag aacgccttca
     1981 tagtgtcatt gagtatgtaa ttatttcttg ggaaatttgt tttaatttta aaaaacaggc
     2041 ttacttcata taatttatga aataaacctg tcaattttgg attgattatg ctttgtgatt
     2101 ctttttattt ctgcgtaata acgctaaacc taaaatgcta aataatccgc cgaacaacat
     2161 gccgttgttt gttgattctt ctccacctgt ttcaggtagt tcagatttct tagattgtgc
     2221 ttttttagtt ggtaccactg ctttaacctt ttcattgatt tcaataacag gtgttactac
     2281 tttaccttgt tccactggtt tagaaggttt tttaggttct tctttagcag gtggtattgg
     2341 tttaccaggt tcagttggta cctctggcgt tggcggtgtt ggtgtttccg gctcgcttgg
     2401 tacttctggt gtcggtggtg ttggtgtttc cggctcgctt ggtacttctg gtgtcggtgg
     2461 cgttggtggc acgattggag gtgttgtatc ttcttcaatc gtttgttgac cttcattatg
     2521 accacttact tgtggaagtg tatcttcttc aaagtcaaca ctattgtgtc caccgaattg
     2581 ataatttggt ttatctttat ttgtatcttc ttcaataatt tcagtgtgct tattgaatcc
     2641 gtgaatatgt ggcacactgt cgaagtcgat atcaatgata ttaccacctt gttcatactt
     2701 aggtttgtct ttctctgtat cttcttcgaa tgattggtta ccattatttt gaccatgaat
     2761 ttgaggtaca ctatcgaaat cgatatctac gatattgcca ccttgttcat atttcggttt
     2821 atcttcttct gtgtcttcct caaatgactg attaccgcta ttttggccac cttcgtaacc
     2881 taattcactc ttaatatcca cgtggctatt ttcttcgatt tcttcaatca cgccataatt
     2941 accgtgacca ttttcagttc ctaaaccaga atgagaaata tgatgattgt tttcagtaat
     3001 ttcctcgatt ggtccttgcg cttgaccatg ttcttcaggt agttcatcta ctagttcaat
     3061 cagattactt tcagtcgtat attctttcgt atcttcaatt gttgtatgat cgctaacagc
     3121 accagttaca ataccttttg tagaatcttc gtcaaattca actaggttag actcagtagt
     3181 aacctgacca ccacctgggt ttgtatcttc ttcatattca acaacatcag catgatgttt
     3241 tgaattttca tgtgtcgatt cttcaaagtc tacatgaata gaatcttctt cagtttcaat
     3301 ggtaccttct gcatgacctt ctgcaccttc aacagctgta tgatattcaa aatcaattgg
     3361 cttagaatca ttactttctt cgattgtacc agtcaattca tgcttctcca ctggcggctc
     3421 tgatttaaat tcaagttcga taggagtact atgttctata ataggttcct ttagtttatc
     3481 tttgccgtcg ccttgagcgt tattagagta aaatgcaacg ccatttttcc aagttaaatt
     3541 acttgtataa taatagttat aatatccaaa aaggtgtgtt tgaaattcta agttgctagc
     3601 atttgaatca taataccctt catattttat tacataattt ttactttggt ctaaattatt
     3661 aaagtttaaa gaataaccac cattagtatc aaaatctaaa ctcatattat cagtcacatc
     3721 ttcaaatttg ctgacatcat caagctttgc atatacgctt tcagctaaat cgtctgaacc
     3781 aatgtgttta tataccttaa ctgttggatt attaacccct ggtttatttc ctttagttac
     3841 ttgaccagtt actgtcacag agcttaacga ctggttgtta ggtttcatgt acgcaaaatg
     3901 actaaatttc ccatctactt tatttaaagt atcaattcga ccattagctg ttactcccca
     3961 attatctcta actccaccta aatattgaat attaaatatt ttgctaaccg tagtctcacc
     4021 caatttaact tcaacatttt ggttaccttt ttgcgtcact gttgtaggat caataaatag
     4081 atttaaagat aattcagcag ttaaatcttt cttttcttgt acatattctt taaacgtata
     4141 tctaactttt ctttctccaa ttatttctcc tgtcgccata acttgaccat ctgtactttt
     4201 tatctccgga actttacgca gtgttgagat accatgagtt tcaacattat cgcttaatgt
     4261 gaaatcaaaa taatctcccg ccttaattcc ttctccaaat ttccatttat atttcaaggt
     4321 tactctttct gcgttatgag gatttacaac attcgtatct tgtttatgtc ctacaatttc
     4381 actaccttct tctacttcca ctttatttgt tacatctgta cctgtcgctt tagtttcttc
     4441 cactacttct ttctctgcaa ctgctgtaac gtcagttgat cttttcattc ttggtttaat
     4501 ttctgagacg ttacttggtt gagctatgtc aacttgagtt cctgtagttt ccttatcagc
     4561 aactttttcc gatggcaaat caactcgcga agtttctact tttggtgctt gcacagtttt
     4621 cggtgcttct tctgttgtta cttgtgttga ttgtgatggt tgctcagttg atgtcgcgct
     4681 gtatgattgt gtttcatcta ttgtattaac gttatttgta gttgtttgtg tttcgcttgc
     4741 tttactttca gtagctgaac tcccactttc ctctactgta gtattgtttt gttccgatgc
     4801 tgcagcttct ttttcttgtc ccattccaac aacgatcatt gttcctaaga atactgaggc
     4861 cgctcccaat ttgtgttttc ttatgccgta tctaagattg cttttcacta taatattctc
     4921 ccttaaatgc aaaattcatt tatttttaaa actcaataaa tgcaattcta tattgttcgg
     4981 tttttaaaag caatgaaaaa aagcgagtta ataaaaagtt aagattgttg ttaactttat
     5041 gtataatgag ttttttatta tttgaaactc acatatatat tgcatacaaa gctcttgaac
     5101 accttgatat aacaggcttg tattttttat acttactttt taaattaaat tcaaattcat
     5161 ctaatttaaa acaatatact aaaccataca taataatcgc ctgtacaatg catcattaac
     5221 aagtcactga aacgcctttc attgtattaa taacgtcact ataattttta tatcgttcgg
     5281 tttttgtttg attttaatga ttatttatac aaaaacagcc gtatttcaag ccgacatttt
     5341 aaatttaact aaatttgcat ctagttaata attgcattta tcaaatttgt cttattgatc
     5401 caatctaatt tgtactcaca aactagttta aaattctaac tttatctctc agttcgttat
     5461 caatcatcag acataaacca atgaagcaat cagaaaacac tctaattttc tattagaaat
     5521 ttgatttaat ataaaaaaac aggcttactt catataattt atgaaataaa cccgtcaatt
     5581 tttgtttaat tatgctttgt gattcttttt atttctgcgt aataatgcta aacctagaat
     5641 gctgaataat ccgccgaaca acataccttt gtttgttgat tcttctccac ctgtttcagg
     5701 tagttcagat ttcttagatt gtggtttttt agttggtgcc actgctttaa ccttttcatt
     5761 gatttcaata acaggtgtta ctactttacc ttgttccact ggtttagaag gctttttagg
     5821 ttcttctttg gcaggtggta ctggtttacc aggttcagct ggtacctctg gtgttggcgg
     5881 tgttggagtt tctggctcac tcggcacttc tggtgtcggt ggtgttggtg tttccggctc
     5941 acttggtact tctggtgttg gtggcgttgg tgtttccggc tcacttggta cttctggtgt
     6001 cggtggcgtt ggtggcacga ttggaggtgt tgtatcttct tcaatcgttt gttgaccttc
     6061 attttggccg cttacttttg gaagtgtatc ttcttcaaag tcaacactat tgtgtccacc
     6121 gaattgataa cttggtttat ctttatttgt atcttcttca ataatttcag tgtgcttatt
     6181 gaatccgtga atatgtggca cactgtcgaa gtcgatatca atgatgttac cgccatgttc
     6241 atacttaggt ttgtcttttt ctgtatcttc ctcgaatgac tgattacctt tattttgacc
     6301 atgaatttga ggtacactat caaaatcgat atctacgata ttgccacctt gttcatattt
     6361 aggtttgtct tcttctgtgt cttcctcgaa tgactggtta ccgctatttt ggccaccttc
     6421 ataacctaat tcactcttaa tatcaacgtg gctattttct tcgatttctt caatcacgtc
     6481 ataattcccg tgaccatttt cagttcctaa accagaatga gaaatatgat gattgttttc
     6541 agtaatttcc tcgactggtc cttgtgcttg accatgctct tcaggtaatt catccactaa
     6601 ttcaatcaga ttactttcag ttgtatattc tttcgtatct tcaactgttg tatgatcgct
     6661 cactgcgcca gttacaatac cttttgtaga ctcttcgtca aattcaacta agttagactc
     6721 agtagtaacc tgaccaccac ctgggtttgt atcttcttca tattcaacaa catcagcgtg
     6781 atgttttgaa ttttcatgtg tagattcttc aaagtcaatt ggatttgatt cctcagagga
     6841 ctcagtgtat cctccaacgt gacctgcttc gctatccaca gcagtatggt aatcgatatc
     6901 aatagctgat gaatccgttt cttctattgt ttcaatgtat ccatcaacat atccacctcc
     6961 accatctata gctgtgtggt aatcaatgtc aagagttgat gaatcatatt cctcttcaac
     7021 agtagttact aaattcttat catattgacc tgtaagagtt tctttaattg tatcttcttt
     7081 atattcaaat ttattatttt gaataatcgg accatttttc ccatttccgt tcgctttatt
     7141 actgtataaa actaaaccat tatcccaagt taaggtatat cctctatcat aataatactt
     7201 ataaagttgc tctggatgtc ctaccatttg tgttctaaaa tcaacttcat cagtaccatt
     7261 taaatactct ccatcatagt gaacaacata agttttatct agattttcta tattcaatga
     7321 atagcttcca ttattttgta aattcaaatt cccactcata ttacttgtga cttctttaaa
     7381 tttagaagta tctgtcgtat ttgcatatac actcttcgct atgtcttcat tattacccaa
     7441 gtattcaaat atcctaactt ttggttgatt tccattctga ttactacctt tcattaaagt
     7501 tccagtaaca gtcacacttg tcgttttacc attattaggt ttaataaatg caacatgcga
     7561 aaatctatta ttcgctttat taaatgtctc aatcgatcca tttaaattgg cataataatt
     7621 cccaatacca tctttatatt taacatctaa ttcctttgaa gtttgttctt catttagtgt
     7681 tgaagttata gtttgatttc cattagtttg tacagtttta ggatcaataa ataaattaat
     7741 ttctagttca gccgttacat caaccttatc ttcaatatca tttgtaaatg tatatctaat
     7801 ctttccacct tctaaaactt cacctgtcgc cattacgact gaaccatttt taatttctgg
     7861 tacttttcta gcagttgata cgccatgcgt atttacatta tttgataaag taaagtcaaa
     7921 gtagtcacct tgatgtaaac cattctcaaa tttcaactta tattttagta ccgctcgttg
     7981 tcctgcatga ggttctactt tatttgtatt gttatgcccc tcaatagaac caatttctac
     8041 tgtaacttta cttgttacat ctgtacccgt ttccactttc gcgttactag cttccttagc
     8101 ttccgctaca tctgctgatc ttgtcacacg tggcttactt tctgatgccg ttcttggctg
     8161 tgccacttca acttgtgttt ctgcgacttg attttgtgta gcctttttag gtgttaaatc
     8221 tacttgtctt tgatctccgc tattgtcttg agattgtgtt gtttccttaa cttgaggttt
     8281 cgcttcttcc ttaactacct cttctttaac tgtttctata tttgctggtt gtgcagtttg
     8341 tggtgcttgt actgcttttg gtgcttcttc agttgttact tgtgttgcgt ttgacggttg
     8401 ttctgttact gttgcgttat atgattgagt ttcttctata tgattaacgt tagttgcagt
     8461 tgtttgtgtt tcacttgttt tattatcagt agctgaattc ccattttctt ctactgtagt
     8521 tgtcttttgt tctgatgctg cagcttcttt gtcttgtccc atcccaacaa cgatcattgt
     8581 tcctaagaat actgatgctg ctcccaattt atgttttcta atgccgtacc taagattgtt
     8641 tttcactata atatctccct ttaaatgcaa aattcattaa ttttttaaac ttaataaatg
     8701 caagtctata ttgttcggtt tttaaaagca atacaaaaaa gcgagttaat gaaaagttaa
     8761 gattgtcatt aattttatgt taattgataa ttttattatt tgaaatatac ctataaattg
     8821 tattcaagtc atcagaaacc cttgtcacac aaggcttgta ttttttatac ttattttttt
     8881 aaattaaatt catcattatc taatttaaaa caatatacta aacgtttcat aattatcgcc
     8941 tgtacaatac gcacaaaaac atgtcttgaa acgcctttca tcactctaaa atacccaata
     9001 tactttttat atcgttcgga ttctgagtat ttcagacgat tttctgcata aaaataaacg
     9061 tgtttcaagg caatatattg caattaccta aaaacacgtt tacttaatat ttagttaaac
     9121 aaataagcta atgaataaaa tgaagatgat acctgaaacg gaaataatcg tttctaataa
     9181 tgaccatgtt aagaatgttt cttttacagt taaaccaaaa tattctttaa acatccaaaa
     9241 tcctgcgtca tttacatgag acaaaatcac actacctgca cctatcgcaa gtacaactaa
     9301 tgcaacattt acatctgatg attgtaataa tggtaagaca atacctgtag ttgaaatcgc
     9361 agctactgta gccgaaccta atgcgatacg tagcacagct gcaacaatcc atgctagtaa
     9421 aatcggagac atctctgtac cttcaaacat tttagcaatt gtatttccga caccgccgtc
     9481 aattaatact tgtttaaatg taccgccacc gccaataatc aataacatca ttccgattgg
     9541 ataaatcgca ttcgtcactg attccataat atgattcatc ttacgctttc tcattaatcc
     9601 catcgtaacg attgcaaata atactgctat tagcatggct gtccctgctg ttcctatcat
     9661 ataaatgata gattcaaata gatttgtagg tttgtcatgc ccagttacaa gttgcgttat
     9721 cgtagacact aacattaata tgactggtaa tgttgctgtt aataaactca taccaaatcc
     9781 tggcatctct tgatccgtaa attctttttg tgcacctaac gctgaaatat cgccttctcg
     9841 tgtatacgca gacggaatca ttttttgtgc aactttgtta aatataggcc ctgcaatgag
     9901 tgtaactgga atggcaataa tcataccata cagtaataca tctccaacat ttgcctttaa
     9961 ttcttttgcg atgactaccg gtcctggatg tggtggtaaa aagccatgtg tcactgataa
    10021 agctgttacc ataggtagtc ctagttttaa cactgaaaca tttgcgcgtt ttgctactgt
    10081 aaatactaat ggaatcagta agactaaacc tacttcaaag aacaatgcaa taccgacgat
    10141 aaatgctgca acaagcattg cccattgtac atgtttttga ccaaattttt gaatcaacgt
    10201 gtctgcgatt cgagttgcac caccaccatc agcaagcaat ttcccaagta tggcacctaa
    10261 accgaatatc agtgcaatgt ggccgagcgt actgcccatt cctttctcaa tcgtctccat
    10321 aattttagtc aatggtatac ctagcattaa cgctgtaatc atcgatgtga taattaatga
    10381 aataaatgta tttaatttaa acccaataat taatactaat aaaataacga tacctaaaac
    10441 aacactgatt aacggccata tttcgttaaa catgacattc ccctctttct cttttcaata
    10501 gaatgtaaca ccgtcgtcgc gcacgtattc cacataatat atagtagtca tggcacaaca
    10561 actttatcta agatgcttga gtgacgtatt tattgtgttt tattttcagc gatatgttgg
    10621 cgttgaaaat ctgcaatttg ttcataattc tctgttaaag aacgacttaa attgataaaa
    10681 atggatacga tctcttggta aacagtgaca ttttcttcaa tcggcgtatg attgtttgtg
    10741 gcaccgacca tcgatgaaac gattgaaaaa tcttcaatgt cacctacagc tttaagtccg
    10801 agcacgcagg cacctaagca tgaactttca taactttcag gaaccactaa ctctgtgtca
    10861 aatatatctg acatcatttg acgccatact tcacttttcg caaaaccacc tgttgctttt
    10921 atcatcttag gtgtttcatt cattacttca ataagcgcaa gatagacggt atacaaattg
    10981 taaagaacac cttctaatgc agcgcgaatc atatgttctt ttttatgaga taaagttaaa
    11041 ccgaagaatg aacctcttgc atttgcgttc caaagcggcg cacgttctcc tgctaaatag
    11101 ggatggaata ttaaaccatc tgcacctggt ttaacacgct ttgcaatttg agttaagaca
    11161 tcataaggat caacaccgag acgtttcgca gtttcgactt cactcgctag caactcgtcg
    11221 cgcaaccatc tcaatacgac accaccatta tttacaggac ctccgatgac gtagtggtcc
    11281 tctgttaaga cataacaaaa tattctacct ttgtaatcag tacgcggttt atctatcaca
    11341 gtacgaatcg ccccagatgt accgattgtg acagcaactt ctcctttacc aacactattg
    11401 acacctaaat tagaaaggac cccatcactc gcaccaataa caaacggtgt atctttatta
    11461 agccccatta atgttgcata acgttctttc atacctttca tcacatacgt tgttggaact
    11521 aattccggca acatttcctt ggaaataccc agcagttcta atgcctcaac atcccaatct
    11581 aatgtttcta aattaaacat ccctgttgcg gaagccattg aataatcaat gatatatgta
    11641 tcaaataaat gatagaaaat gtatgtttta atatctgcaa acttagcagt acgttgaaat
    11701 acatcttgcc attcatgttt catccaaaaa atcttcgcta atggcgacat aggatgaatc
    11761 ggtgtgcctg ttcgctggta aatcgcattg ccatcatgca cttcatttat tactgttgca
    11821 tattttgcag cgcggttatc tgcccaagta atattatttg ttaatctttg atgttgctga
    11881 tccatcgcaa tcaagctatg catttgcgca ctaaatgaca caaacttaat gtcgtcttta
    11941 ttaactttgg attctctcat aacatattta atagtcatta gtactgcatc aaataattca
    12001 tctgggtttt cttctgagac atcaacgttt ggtgtgtgta aatcatagcc tatttgatgt
    12061 ttcatgataa aagttccatt ttcatcatat aagactgact tggtactcgt cgttccaatg
    12121 tcgacaccaa tcatatattt catgataaat ccttctttct ttcattttaa ttcaaccaaa
    12181 atccttcaat atctttacca acatcgtcga aatttaaatg aaacgcttct ttcaaaattt
    12241 gactgtcgta ttgttccact gcatcaataa acacttgatg attatgatgt atgcgttcaa
    12301 aatcttgcgg gttctgttgc atacgttgtc gcatggatgt taaaacgagt gccatcatta
    12361 ctggttttaa atgactccaa aatgagttta aatattgatg attagatgct ttgattaatg
    12421 tttcatgaaa ttcaaagtca tgcttcgtaa atgattctgc atcctcaaat tttactgcca
    12481 ctttcatcat ttcaagttgt ttcttcattt cttttacgat gggtagtcgc tcttgatttt
    12541 taactcttga aaatgcaaat gactctaaca tcagtcgcaa atcatacatt tctttctttt
    12601 cttgttcccc aaacggcaac acatgtgcac ccattctttc taattggatg agttgatttt
    12661 gttgcaataa tttaaatgca tctcgaattg gcgaacgact cacattaaat tgctttgcca
    12721 tttgattttc agtgagtaac gtaccttcag ctatgtgacc attcacaatg cctaagcgta
    12781 attctgccgc gataccttct ccagttgtca taccttccaa ccatttctct ggatatccat
    12841 acatcatcaa agtcactcct tcattacacg acatacttgt atacaagtat gttaatatag
    12901 ttattatgag tttgcaagcg ctttctttac gagcactaaa atagtgacca ccccttttcg
    12961 atttaaattt aaaggaaatg gtcactatca cacgaatgat ttaattgtta tgttgtatgt
    13021 gggatatttc taattgttct gtactcatat gcgctttagg tacttcaatg caataatgcg
    13081 tttcatgaca gtttggacat tcgaatcgac gtgttgtcgc tgtatgtttc gctttgataa
    13141 ctgcccacaa agatggtgag aatatatgct ggcagttagg acataaatag gcaacctttt
    13201 gttggtaata aaaagtaaca ccaatgccat aaccaatcat aaatggtaaa gcaattaaaa
    13261 acggccattt atttttcatc aaaattgcac ttataatgct agaatattga attattccta
    13321 taataccagc actaatccaa atgttacgac gaatactttt catttcagct gatttactca
    13381 tgacatgctc tatgtctttt aagtgtgtga ttggagacgt cgacgcttca tttacgtaat
    13441 attgaacatt tttaattttg tttaataccg cttgttgctg tttaacttgt tggttaattt
    13501 cttgttgttt catagttagt aaagtattga gcgtcttcaa agtaccttca ccttttagca
    13561 acatatctat atcgcttaac gcacaaccta aatctttaag caataagatt aactctaatg
    13621 tttgtcgctg ttgttctgta tacacacgac gctttccttc tgtaaatcct tgtggtttca
    13681 aaataccttt gcgatcataa tattgaatcg ttcgtgttgt cacattgcat aattttgcga
    13741 gttctccagt cgaatagtta gacatagatt ccacctccta taattaccat agttgatgac
    13801 ccgacgtcac gagcaagtac aatttccaca ttttaaagaa atttattata ctaggcgtct
    13861 tatttttatg atttcgtacc atgttgattt acaaactcac tcaaactaag taacacacct
    13921 actaaacatc tactctgtta tttcagaatg aatttgttgt aatttatctt caacttcagt
    13981 aatctctgtc gcacattctt tcagtaaatc tcgatacttt tccgtctctg cattgttttt
    14041 ataacgtatt ttatgttcta aacttgccca catatccata cctatcgttc taatttgaat
    14101 ttcaacaggc aatacctcaa cgcgttctgc taaaaagact ggaatagata caacgatatg
    14161 taaactgcgg taaccatttt ctttagggtg ctgaatataa tctttacgtt ttatcaattg
    14221 tacgtcttct tgtttaagca acatctcttc aatcaaataa atatcatcta agtaattaca
    14281 tactacgcga attccggcaa tatccagtat atgttctttg gcagtttctg cgctaatctg
    14341 taatccttta cgattaagct tttctattaa actacgcatt tctttcacac gtcgctccat
    14401 atgatgtata ggattgtgtt tataaatgtg attgaaattg tcatctaaaa tactcagttt
    14461 agtgcttatt tcctttagtg ctgacgaata aatatgatct aactctacga aacctaataa
    14521 cgtatcaaat gcttcatcac cattttcaaa tttacttaag ctatttttaa aatcatgtcg
    14581 caaatcctct aaatatagtg atggttttcg atctacatac atttttatac taacctccga
    14641 tatataatca tctttattgt acctaacatt ttattaagat aatactaata cactatcgaa
    14701 ctttgggctg atactgaaac agcacaaaga aactcaaaca attcgaatta tgtatcaaaa
    14761 ccttcaatta caggaagtga ttttaaaatg atttaacgca aaaaaacacc tgttaccgtt
    14821 atataggtac aagtgcttaa ttgatagaga tgttatacgt ctttaaatga ttccacaact
    14881 tttggatgtg gaccatccat aagcggttct ctttgtcgaa cgccaccact ttggttgcca
    14941 attgattcgc tatcaaagta cgatttatct tgatttgatt gttcttgaat atgttcttca
    15001 ttatcagttg ttgcatattc actatccacc tctgtttttt ccattgttgc tgtatggaat
    15061 tgtacgaagt tctcttcttc taatgctttg atttcttctt tcgataacgc tcgataccaa
    15121 tctttcgctt ttttcgccgc aataggaaca acatcttttt caagatatcc ttcttttaac
    15181 attgcattcg caatttctat cgctttttgc cgtttaatta cttcaaattc tttccatttt
    15241 tcggggtaac ctttcattgt aaagggcatt tccttaacct ccaatatgtt ataattcatg
    15301 ttatatacca ccataattat aaaatgaaac atattaaaca cattttttta gactcccgtc
    15361 ataataactt cagacaaacg ttagtctgac ctgataaata tttaatttta aacgttcttg
    15421 tctctctcac gaaaaccatt gtgttgaata ttcatcattg ctgattgaat ataattatac
    15481 tgtggtaaat ctggtaatat ctttatttct aattctgtat ttaattcgaa atgcttagcg
    15541 atgttttcaa atattgctaa atactcgccc attaattgtt catttattgt aagtctatct
    15601 tcattagcca tggctctatt taacataaag ctaatttctt ctaatgcgaa taagctagga
    15661 taataatttt gaatcaatgt cttatcacta aataattcac cattagctgc attataaact
    15721 tgcgtcatgt tatttaattt cacagatagg ttcatagccg tgtttttctg atacacatca
    15781 tctttatatt gattttcaga aaataaataa tgaaagagcg ttgcttcttt acgaacaact
    15841 tcagccatta cattaggaag caatgcggat gcggtacgtt gtccaaggac aaataaacct
    15901 atgattgcaa tcacaatacc cattgcaacg tcaataactc ttggaaacgc aatgttaatt
    15961 gttaaattct gtgatgctaa tccgttcatt aaaataactt gtattgtaat aaaaactact
    16021 gcaatcgcat aatttgctcc caccaatgct tcagtaaaca atgctgcaat gcccattaaa
    16081 attactgcaa caggtgttgg tattgaaaac aacaatatga ctgaaagtac taacacacct
    16141 aaaatagtac ctaaacctcg tgccatacct ctctcgattg catgtatagt tgatgttcct
    16201 agtaatattg tatgtgcaga taacggtatc cagtatgctt tttcaaaatc aaacattaac
    16261 gcaataaata tcgctatcat cataatcgct gtatatctca atgtatttct aaaaacaata
    16321 gagtctaatg ttaaattttg ataaatgcgt ttactatata aaggttttcg catgtctgct
    16381 tcatacgcaa tacgcgcttc atttgcatgc accatttcat caattttcaa tatatggttg
    16441 aataaatttt gaaattcctc tgttactgtc acttcttttc gccaatatct tacattttcc
    16501 tcactattat ctagttgtgc aatgatatga tccatcattt caattaactc atctggcaat
    16561 ggtcgaattt gtttagcgtt caactctaac agttcagaat aaatcccttg ggcagatgtg
    16621 tgtaataata ataatttttg gaaacgtcta cttaatttat cgttacttga agtagaagtg
    16681 attaataatt tatcagatgc tttaaaacta tccaccgctg tttttgctac ttgtaaaaaa
    16741 gcagatttat cattataagt gtgtaacaac ttagatatga gtttaaaatc attttgaatt
    16801 gcttgttctt cagctttgtt tttagaaaat acgatcgtga ttaacaccat catagtggca
    16861 agtataccgc ctactaaaat cgcaaagcct ctatataatg cttcttcggg agctataggt
    16921 aagttaatgg gtagactgaa tgtcacaatg aagaatgtcg atgacggtcc agctattttt
    16981 aaggcagtaa atatataaaa tggtataacc gtaacaatta ataatgtcat tccaaaaacg
    17041 agtggcgttt tggctgttag cgtaccaagc atcatacata ttgcaaacgc taaattacaa
    17101 attattacag ttcgcagctt agatcgcgac ggacctttaa aaacataaat atgggctagt
    17161 gttccggttg caactaataa tccaaattgg aaattaccac ataagtaacc gattattgca
    17221 ggaataatca ttaacagtcc ctgtcttaca cctttataaa agtctaagtt aagtcgattg
    17281 atttttatga gtgatgtcaa aaagatgtca tttcgacttc tcctttcctt gtcaaaatat
    17341 ttccataatc tttttattag catagttaaa atgaatttgt tgctataaat tttaattacg
    17401 caacatatgt cattataaca ccttatgctc aattatctaa atttaaaaac ttttaaagtc
    17461 caattaaaat taatttctaa tgttatttaa catcgttgaa cctttggtcg cccacctttg
    17521 cgctagttaa aatttatatg tacttttaca agcgttacct gactaatgtt ttaaaacctt
    17581 atttctttac atatatcatt tcaataactt cattttcatc ttaaaccata tctgttttaa
    17641 aaagtttcaa tttcaacgca aaaaaagcaa taagccccaa ctatgtgaaa gcttactgct
    17701 tgtttaaatt atttttctat tgctttatca tcactaacaa ttaaaggttg atctggtttc
    17761 acatagaacc acataatcac agcgattact gctggaacga ttaacaattg gaatgtcatt
    17821 gtccaaccca aatgttctac gaatagacct gctaatagcg gactgattaa tgcacctata
    17881 ttaccccata agttcatcca ccctgatact gtaccagaga agttacgtcc taagtcagtt
    17941 gctgcagccc aactcatacc cattgaaata ccgataccac caagacctag tgataaccag
    18001 aaaatactta catataaatt tcctgtgcgt acagcaaaga taattgaaac tgcaaacaca
    18061 ataaatcctg caattgcaat tacacctcta gctacgaatt ttgaacgacc taatcctaat
    18121 acacggtcag aaattgcacc tgctgataag attaagaaga acattaataa ccaaggtaat
    18181 gaactaatgc tcatttcttt aaagttaacg tggaatactt ctgttaaata cgtcggtaac
    18241 caaattaaga ataatgtaat gataaattgt acaacaaagt attgaattgc aattgcatag
    18301 aagctaaaac gtttaaagaa atcattccat ggtggtgatg acttttcagt agctacgata
    18361 tcacgatttt ccataatgaa acgtttctcc gcttcattaa ccattctatg ttgctcaggt
    18421 aagtctttgg caataatcgc ccataataca gccattaaaa tacctactgc accaaaaatg
    18481 taaaatactg cttgccagtt aaatgcgtta acaatagcaa ttgtaactat tggtgctaat
    18541 acaggtccga aatatgatcc tgctaataat gcacttgatg ctctaccttt ttcattttta
    18601 gagaaccaaa atgaattaaa cacagcatta gaagggtaca ttggcgcctc acctacacca
    18661 aataagaatc tcactaaata aattaaaccg tggttcttaa tcatacccgt taagattgta
    18721 aatgcactcc accaaactaa tgcaattgta atcatcttac gtggaccgaa tttttcagcc
    18781 aacatccccg atggtacttg cattaatgca taacctagag agaagaatga tgccaataaa
    18841 ccaaattgtg gttttgtcat ccctaaatca tccatcattt gcttagcgat atatgaaatg
    18901 tttgctctat ccatatacgc aataacccca ataaagaaga acgcaagcgc gaacatccac
    18961 cttacgttag ttcgtttttc cttcataact attcctactt ccctaattaa ttttttaaaa
    19021 cttaccctga aacgttaaaa ttttcggaca tctggcatgt ccacccctac tttgtcacaa
    19081 aattgtacca aagttatttt ttataatatt atcagacatg gaaagcgttt acaatagaat
    19141 ctataaaaat tttattaaat tccgcttaac ttttgctatt aaaacgatat aaaggtactt
    19201 ttagttacat agtagcctat cgtttgttaa ttaaaaatac tgcaatccta gtgttttaaa
    19261 acgtatattg caggctttct tacatattcg acattcattt taaattgcat ataactacat
    19321 taaccattca ttgtgcagcg cctgttgttt agatgatcat ttattgactt tttgctctta
    19381 tagcaagcaa ttttttgatt tattgcttat aatacatttt aagacaaatg tttgtttcaa
    19441 catcgcatgc gacatcatta attattcatt tgaatttatc cagtttctat ttgtctgcaa
    19501 ttgctgaaaa ttaaaggagc ttatttttaa taatggaaca aattatcact gaatttatta
    19561 gccgttttgg gtatgcagcc atttttatat taattttatt agaaaatgta ctacctatcg
    19621 taccatcgga aattattctg acatttgctg gtcttatgtc tgttaaatca catttatcaa
    19681 ttttaacttt atttattatt gcaactatcg catcgtttat agggctgtta attttatatt
    19741 atatctgccg tttgatttca gaagaacgtc tatatcgttt tattgatcga cacggtaagt
    19801 ggattaaatt gaaaagtaag gatttaaagc gagcaaatga ttggtttaaa aagtatggcg
    19861 tatgggctgt atttatctgt cgtttcatac ctgtattacg tgtattgatt accattccag
    19921 ctggtgtaaa ccgcatgaat gttgtgacat ttaccgttat ttcattaata ggtactacaa
    19981 tctggaattt cggtttaatt ttactaggac gcactttgag cgatagcttt ggtatgttga
    20041 tgactggtct tcatacatat tcacgtatta tgtatgttgt catcattatc gcagtcattt
    20101 actttgctat acgttacatt ggcaaacgta agagagtaaa ataatattgt tgttagcata
    20161 aaggcatgaa ttttaaattt ctataaacaa aaagcagtga atcattccat ctattgaaat
    20221 gattcactgc ttttttaagt ttatttggca tactttagct gaaagtcaac tatctttata
    20281 taaagaaata ttactattaa catgatagtt atagttgtga agtaaaaggc taccaacggc
    20341 tgagaaatga taccaattaa taatgcaccg acagtcattc caatatatct cacaaaatta
    20401 aatacaccta gcactttccc ttttatatgt tcaaaccttt tagaaattat agtagacagc
    20461 aaagctggca caataccaac acaagtacct aatataaata taattactga taatataatt
    20521 acatttgatt cgtttaacca tccaaacaat aacgtaaata tagcaaatcc agttatagta
    20581 acacgaagta tcattatttc attatatttt tttgaaaatc gcttataaaa taaacttcct
    20641 aatataacac taacagtaat aggtaaaaac aaaactcctg atattccaac gcctatatca
    20701 taggtattat ttaataatgt tggtaaataa actaatattg aaaagtacgt aaaaaatatt
    20761 aaaaatccca atgtcactaa agtaataaac actttatcgg tcaaatattt ttcttcaatt
    20821 ttttcagtta attttacaat ccctttttca acatttggaa tatcaataaa aaagataatc
    20881 aaaaataata cgatagatat aacgagcaaa aaactaaaaa taccaatata atcccatctt
    20941 gcacctaaag tactccctaa gattggtgcc aaagctggtg cacatgataa aaatatttga
    21001 tagttcgcca tagcttgagc tctaccatta tctgtactta gtttggctaa taaagttaaa
    21061 gtcacgagag gaataattcc gcaaccaatt gcttgaaata accttgatat agcaaataat
    21121 ataaagttat gtgtcactgc acaaataaag ctagatatga ttactattcc caatcccgtt
    21181 aaaagtactt ttttcgaatc acgagaatct atacttctac caagtacaat ttgcataact
    21241 gcaacgataa atataaaacc gccaactgtc gcatttatcc aaaatagtgg tacttgaaaa
    21301 tcgtcatata gtctcggaat aataggcgtg tatatgtttt gaatcaatga tgcgaaaaat
    21361 gctaaaaatc ctagaatata taatctgata ttactattaa tcatttaaac catcttcttt
    21421 ttttaattct tctatactac ggtttaaaaa ctctataata actgccattt catcatcatc
    21481 aaagacttta agtacctctt catatttttt taccgatttt tcatgtaatt catcatgtat
    21541 ataagataat ttttttcctt tttgtgttaa tgaataatta acttctcttt tgttaaattc
    21601 attgtgcgat tcaactacta agtctttcgc taaaagtttt tttatcgatt tagtaattgc
    21661 tggttttgat acatttaaca tttcagacaa aaatttatta ttcactttat cattattatc
    21721 aatcaactct atgatatgaa attgtgttag agataaatct agttcctcat tttccttttt
    21781 atactgtctt cttttatcgg cattttctct ttctataata aattgttgta attttaaaat
    21841 aacttctctc ttcatatcca ctattattcc tcctcttcat tagttaaccg gttaactata
    21901 ttattgttgt cactcccatt tttgtcaaat attgttgtaa ttaacatata tttacttagt
    21961 tatgcaccat ttattccgca tttcacaaat ccacctataa tacaaactat cattttaaat
    22021 aagcagaatt taccctgtct tccacacaaa ttgttttcga cactttaatg tgcctactat
    22081 ccaaaaaagt tactcataac aaattgcgca ttttaataaa tgtagcaatg ccatttgcat
    22141 gctgcaaaaa tactcgtatt catatttatg ttttatatta ttaaagtttt ttaaatccat
    22201 atttttataa cacttgctat gtgataaaat taattttata tataaaatct taagattcag
    22261 attatttaat agcaaaggag atagtgatat ggatgtttta acaatagaac atttaacaaa
    22321 gaagataggc aacaaaacga ttctcgaaga tgtatcattt aagctgaaac gcggacaaat
    22381 agttggtctc gttggagcga atggtgcagg taaaacaact ttaatgaaag ttatattagg
    22441 ttactctagt ttccaaagcg ggaattttaa tgttattaac agcaaggaca gcaaaagcaa
    22501 tatcggcgca ttgattgaaa atccaggaat atatcctttt atgtctggat atgaaaactt
    22561 gaagttattg aatgaatcaa aaaacactca agatatcgat aaaattgtct cacaacttca
    22621 tatggatgaa tacattcata aaaaagctaa aacgtattct cttggtatga aacaaaaatt
    22681 aggaattgct atagcatttt taaataaacc tcaattcatt atcttagatg aaccaatgaa
    22741 tggcttagat ccaaaagctg tgcgagatgt acgtgaattg attgtccaaa aagcgcaaga
    22801 aggtgttact ttcttaattt cgagtcatat tttaagtgaa ttagttaaaa tcacaaactc
    22861 tatccttatt attaacaaag gtaaaattgt tacagaaaca tcggaagaag aacttaaaca
    22921 atttaaagat aatgatttag aaaatgtatt actagaaatc atagaaaggg aggaccaagc
    22981 ataaaatggg aactttaatt aaacaagaat gtttcaaatt atttaaaaag aaatcaactt
    23041 ttatcgcacc tattgtcttt attctactaa tggttgctca aggttatatt gctacaaaat
    23101 acaatgaaat ttttacgcca caggaatctt tcacatctgc ttataatggt ttttcatggt
    23161 ttgcattttt attaattatt caagcaagta caatcatttc aatggaattt cattacggta
    23221 cgattaaaaa tttactctat cgtgaatatt caagaacaac tatgattgtt agcaaaatca
    23281 tcacattatt tattatttct ttaatttatt ttgttattac aattattgct tcaattgtta
    23341 ttgggtcttt attctttaat gatttaaata tatttgaaag tagcggtaat caattatctt
    23401 tattgaatca attattatta gttagtttag gcacatttgt tggcgtttgg ttagttttaa
    23461 gcttaacgtt gctattatca tctgcaacaa attcaacggg agtagccatt gctgtaggta
    23521 ttgtttttta ttttgcaagt tctattttag cagttattca aacggcactt ttagaaaaaa
    23581 tagactggct aaagtggaat cctattaata tgatgaatat tatgcttcaa acagttgaaa
    23641 aaggctttag taagtcgaca aaattagaac ttcatgaatt gtttattggt aatattgctt
    23701 atatttctat tttcttaata cttgtagtat ttattttcaa gaagaaaaat atttagtaac
    23761 ttaaagtatt aaatgtctaa atacacacat attccatcgt aattcaaaat cattttcaaa
    23821 tcccttcacc caaataatgg tgcggggatt ttttcatcca aattttggaa attccacatt
    23881 ttacatatcg taatttttta ggaaactagt gaatataaca aatccctcct ctcattttta
    23941 aaatagatat atcacttccc cacttttact taactaaact gcaacggttc ctaataccaa
    24001 aatcctgccc tctattttta tcaattcaag catacttatt gaaaaatgtt aacgttttct
    24061 tgataatcat tgtaagcgca tttattttat aaactaacgt ttgaaatata ctacaggagt
    24121 gacacgtaat gactcaaatt actgaaaaag aattaaaaaa gaagtattta gatttactat
    24181 cccaaaattt tgatactcca gaaaaacttg caactgaaat tatcaattta gaatcaattt
    24241 tagaattacc taaaggtacg gaacatttcg tcagcgattt acatggtgaa tatgaagctt
    24301 tccaacacgt attacgcaac ggttctggga acgtgcgagc gaaaatcaat gatattttca
    24361 aagagagact ttcaactaag gagcttaatg acttaactgc tcttgtctac tatccagaag
    24421 acaaattaaa attgattaaa agtgatttcc aaaattgcgg tcaacttaat gtctggtata
    24481 tcacaacaat cgaacattta attgagttaa ttaaatattg ttcttcaaaa tatacgcgtt
    24541 caaaactgcg taaagcattg ccaaaacaat acgtttatat tattgaggaa ctactgtata
    24601 aaagtaatga atatcaaaat aaaaaatcat attacgaaac acttgttaac caagtaattg
    24661 aacttaaaca ggcagatgat ttaattattg gactcgctta ttccgtacaa cgcttagtcg
    24721 tcgatcattt acacgttgtc ggtgatattt atgatcgtgg accacaacca gataaaatta
    24781 tggatacact gattaattat cattccctag atattcaatg gggtaatcat gatgtgcttt
    24841 gggttggagc ctatgctggg tcaaaagtat gcttagcaaa cttacttcga atttgtgcac
    24901 gttatgataa tttagatatt atcgaagacg cttatggcat taatttaaga ccactgctta
    24961 ctttagctga aaaatactat gacgcagata atcctgcttt taagcctaaa aaaagacctg
    25021 acaaacacga acgtttaact caacgtgaag aaagtcaaat tactaaaatt catcaagcta
    25081 ttgcgatgat tcaattcaag ttagaaatac caattattaa acgtcgtcca aatttcgaaa
    25141 tggaagaacg tcttgtgctt gaaaaggtta attatgatac aaatgaaatt acagtttatg
    25201 gtaatacata cccattgaaa gacacatgtt tccaaactgt caatcgtgat aatccagcag
    25261 aattactacc tgaagaagaa gaagtcatga ataaactatt attgtcattc caacaatctg
    25321 aaaaattacg tcgtcatatg tctttcttga tgcgtaaagg ctctctttac ttaccatata
    25381 atggcaattt actcattcat ggttgtattc cagttgatga aaatggtgag atggaatcat
    25441 ttgaaattga tggtcatact tacagcggcc aagaattatt agatgtgttt gagtatcatg
    25501 tccgtaaatc atttgatgaa aaagaaaata ctgatgactt atcgacggat ttagtttggt
    25561 atttatggac tgggaaatat tcgtcactat ttggtaaacg tgccatgact acgtttgagc
    25621 gatactttat tgcagataaa gcttctcata aagaagaaaa gaatccgtac tatcatcttc
    25681 gtgaagatgt gaatatggtt cgtaaaatgc tcagtgattt cggattaaat ccagatgaag
    25741 gacgcattat taatggtcac acaccagtga aagaaatcaa tggcgaagat cctatcaagg
    25801 ctgatggaaa gatgcttgtc attgatggtg gcttttcaaa agcttatcag tcaacaactg
    25861 gcattgcagg ctatacacta ttgtataatt cattcggtat gcaactcgtt gcacaccaac
    25921 aatttaacgc taaagaaaaa atactttccg aaggtatcga tgaactttct ataaagcgcg
    25981 tcgtagataa agaattacaa cgtaagaaga ttcgtgatac caatattggt aaagatcttc
    26041 aagctcaaat agatattttg aaaatgttaa tgcatgatcg ttacttagac taaagacgac
    26101 atttgatgca tgtatcgtgc acttatttag tggtactgtg tttacgcatt ggctacgcaa
    26161 acacgatgat aaataggcga cctagctaca ccgaaaaaaa tcccctcact attgcgagta
    26221 gtgaggggat tggtgtatct atgctaattc ctgttaactg tattataaca actttacagc
    26281 aaaaaaatgc aaaatcaact aataaattaa tgaaatgacc gacaccctcg cttctactcc
    26341 aatttaacaa aatactatat tagtactata tttttcatta acatgtatgt tacattttct
    26401 atattactta aaattacgaa gggacatttt taaatcatgc gtatcttaaa agagtccatt
    26461 attgtggcat ttgcctttgt tggtgttgtc gttggtgccg gctttgctac tggtcaagaa
    26521 attttccagt ttttcacaag tcatggcgca tatagcattt caggcattat tgtaacagga
    26581 ctattgatta ctttaggtgg aatggttgtc atgcatacag gtcatcatct aaagtccaga
    26641 aatcattctg attcaattaa ctatttctta tacccctcta ttgcaagagg ttttgatatt
    26701 attttaacaa tgtttatgtt gtctttagct attattatga ctgcaggtgg tgcgtcaacc
    26761 attcatcaaa gtttcaactt accgtattgg ctgagcgcac tcatattagt cgcctttatt
    26821 ttagcaacac tgtttctaaa attcgatcgt ttaattgctg tgcttggcgg tgttacccca
    26881 tttttaattg cgattgtcat tatgattgcg gtctactatt tcacaacaag tcatcttgat
    26941 tttactgccg ctaataatga tgctcagatt cataagcaga aatcattatc acctggatgg
    27001 tggtttgatg cgattaacta tgcaagcttg caaattgctg ctgccttcag cttcttatca
    27061 gtgatgggta gtaaagttaa atatcgtgac tcaacgttat acgggggctt gattggcggt
    27121 ttaatcatta catttttact catgatgatt aatctaggtt taatttctca attcgataaa
    27181 attaaacacg tagatctacc tacattaaaa ttagcgacac aaatgtctcc gtcaattggt
    27241 attattatgt ctgtcattat gatacttgtc atctacaata ctgttgttgg attaatgtat
    27301 gcatttgcgt cacgtttcag cgttccattc agcagacgtt acttcatcat tattattaca
    27361 atggctgtca tcacttatat tagtacattt atcggtttca tttcattaat tggaaaagta
    27421 ttccctatta tgggattgtt cggtttcatc ttactcatac ctgtactcta taaaggttta
    27481 attaagcgta ttaccggcaa atctcatatc gattaaaata tctatactat tgtaaaattt
    27541 agtacagcac ttcaaaatca aaaaagcggg cagatgaaaa gtaaaaatct tttccatctg
    27601 tccgcttctt aatagaactg ttatttccca agtatttgtt gaccagccgt taatccagtt
    27661 tctgtaattt catggccctt cacccaaact tcttcgactt gtgccccacg tgtattaaac
    27721 aagttaatga cttgttcact tgcagctaat ggcacaattg gatcatgttt ccccatagaa
    27781 agcaacacac tgacatctga taaatccttt gttgacgtta cttcaactgg gtataacggt
    27841 gcatataaca atgctttttt aaatggtgct tctgaacgca acattaagtt aatcgctata
    27901 tttgatccat ttgaaaatcc aacaagtact gctttttcaa tatcaaaatc ataacgttca
    27961 gcagcttctt taatgaacgt caacaattct tgtccacgaa atgccaaatc ttcttcatca
    28021 taaacacctt caccaagacg tttgaaataa cggttcatcc cattttctga aacttgtcct
    28081 ctaatactta acaagtgata attttcattc aatgcttcgc ctaacggtaa taaatcgaac
    28141 tcatcaccac ctgtaccatg caataatatt agtgttggcg caccattttg tccttctcta
    28201 aaaatatgtt ccatcacaaa tcctcctcat tccaattaac catgttgacg cttcgtatta
    28261 aaaggtctaa cttccgattc aatatattct cttttatttt ctaaaaatgg tggtaaggat
    28321 aacccttcgc ctaatgtttc ataaggttca tcttccataa atcctggtcc atctgttgaa
    28381 atttctatta aaatatgccc cacacgtgca tataatgctt caaaatagaa acgattaacg
    28441 atgcctgagt tattaatacc tacctcttta tatttcgttg cccacgcttc tattgcatca
    28501 tgatctttca cacgaaatga cacatgatgt acctcaccat aaccttgacg tgctgctggc
    28561 cctttatcat cttttattaa gattacctgg ccaccattgc ctccttcgcc aactt
//
LOCUS       DALAGD010000028        28041 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.028, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000028 DALAGD010000000
VERSION     DALAGD010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 28041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28041
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.028"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            83..1009
                     /gene="hemC"
                     /locus_tag="O2701_000968"
     CDS             83..1009
                     /gene="hemC"
                     /locus_tag="O2701_000968"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCZ4292873.1"
                     /translation="MRKLVVGSRRSKLALTQSQQFIDKLKAVEPNLEIEIKEIVTKGD
                     RIVDKQLSKVGGKGLFVKEIQHELFEKNIDMAIHSLKDVPSVIPEGLTLGCIPDRELP
                     FDAYISKTHTPLSQLPEGSIIGTSSLRRGAQILSKYPNLEIKWIRGNIDTRLEKLQTE
                     DYDAIILAAAGLRRMGWSDDIVTSYLDRDTLLPAIGQGALGIECRSDDEELLTLLSKV
                     HNDEVAKCVTAERTFLAEMDGSCQVPIAGYATISDQNEIEFTGLIMTPDGKERFEYTM
                     NGTDPVELGKTVSNKLKEQGAYEIIKRLNEQH"
     gene            1031..1699
                     /locus_tag="O2701_000969"
     CDS             1031..1699
                     /locus_tag="O2701_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCZ4292874.1"
                     /translation="MKPVVVMTQTNDMQSDLVSIIHKPFIDIKPLNFDIHLLNQRYDW
                     LIFSSKNAVKFFYKYLKGINVDNIAVIGSKTAQYCESLGIRVDFMPNDFSQEGFLKSF
                     NQTNQKILLPSSELARPLLLAALSKDNEVVKIDLYTSVPNKQNIQDVKEMIEHQQIDA
                     LTFSSSSAVRYYFNEGFVPKFKSYFAIGEQTARTIKSYQQPVTIAEIQTLESLIEKIL
                     ESRG"
     gene            1702..2676
                     /gene="hemB"
                     /locus_tag="O2701_000970"
     CDS             1702..2676
                     /gene="hemB"
                     /locus_tag="O2701_000970"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001633925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCZ4292875.1"
                     /translation="MKFDRHRRLRSSATMRDMVRENHVRKEDLIYPIFVVEKDDVKKE
                     IKSLPGVYQISLNLLESELKEAYDLGIRAIMFFGVPNSKDDIGTGAYIHDGVIQQATR
                     IAKKMYDDLLIVADTCLCEYTDHGHCGVIDDHTHDVDNDKSLPLLVKTAISQVEAGAD
                     IIAPSNMMDGFVAEIRRGLDEAGYYNIPIMSYGVKYASSFFGPFRDAADSAPSFGDRK
                     TYQMDPANRLEALRELESDLKEGCDMMIVKPALSYLDIVRDVKNHTNVPVVAYNVSGE
                     YSMTKAAAQNGWIDEERVVMEQMVSMKRAGADMIITYFAKDICRYLDK"
     gene            2724..4010
                     /gene="hemL"
                     /locus_tag="O2701_000971"
     CDS             2724..4010
                     /gene="hemL"
                     /locus_tag="O2701_000971"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCZ4292876.1"
                     /translation="MRYTKSEEAMKVAETLMPGGVNSPVRAFKSVDTPAIFMDHGKGS
                     KIYDIDGNEYIDYVLSWGPLILGHRDPQVISHLHEAIDKGTSFGASTLLENKLAQLVI
                     DRVPSIEKVRMVSSGTEATLDTLRLARGYTGRNKIVKFEGCYHGHSDSLLIKAGSGVA
                     TLGLPDSPGVPEGIAKNTITVPYNDLDALKIAFEKFGNDIAGVIVEPVAGNMGVVPPI
                     EGFLQGLRDITTEYGALLIFDEVMTGFRVGYHCAQGYFGVTPDLTCLGKVIGGGLPVG
                     AFGGKKEIMDHIAPLGNIYQAGTLSGNPLAMTSGYETLSQLTPETYEYFNMLGDILED
                     GLKRVFAKHNVPITVNRAGSMIGYFLNEGPVTNFEQANKSDLKLFAEMYREMAKEGVF
                     LPPSQFEGTFLSTAHTKEDIEKTIQAFDTALSRIVK"
     gene            4071..4172
                     /locus_tag="O2701_000972"
     CDS             4071..4172
                     /locus_tag="O2701_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292877.1"
                     /translation="MGGDLIVKVHICFVVKTASGYCYLNKREAQAAI"
     gene            4206..5279
                     /locus_tag="O2701_000973"
     CDS             4206..5279
                     /locus_tag="O2701_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCZ4292878.1"
                     /translation="MAMIYRNNFIVFVLSFFISILLYSSHVLLPFMFGPIIASIICVK
                     VFKLDIKWPFLLSELGIVLLGVQIGSTFTKNVVMDIKNNWLSIIVVSISILLIAIVMA
                     FVFKKIARINTETAILSVIPGALTQMLVMAEQDKRANLLVVSLTQTSRIIFVVVLVPF
                     ISYFFHDGNMHANGKLTKVLPLSQVLNIGQIVILAIAIFIVYLIMSKIKFPTFQLLAP
                     LIVLIVWNFSTGLTFTLDHWLLNMAQLIYMIRIGVQIAHLLSDLKGRLAIAITIQNIM
                     LIIGALIMVYIIHFFDNNPINELFLGAAPGGVNQIVLVALATGADVAMITSYHIFRIF
                     FILFIIAPGINYFLKYRSNKRED"
     gene            complement(5502..6062)
                     /locus_tag="O2701_000974"
     CDS             complement(5502..6062)
                     /locus_tag="O2701_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCZ4292879.1"
                     /translation="MNECAFGTKDPVYLDYHDHVWGQPLYDSKALFKLLALESQHAGL
                     SWLTILKKKEAYEEAFYDFEPEKVAQMTAQDIDRLMTFPNIVHHRKKLEAIVNQAQGY
                     LKIEQAYGSFSKFLWSYVNGKPKDLQYEHASDRITVDDTATQLSKDLKQYGFKFLGPV
                     TVFSFLEAAGLYDAHLQDCPSKPKHN"
     misc_binding    6120..6421
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6465..9095
                     /locus_tag="O2701_000975"
     CDS             6465..9095
                     /locus_tag="O2701_000975"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCZ4292880.1"
                     /translation="MEMKPKYDPREVEAGRYEEWVKNGYFKPSEDKSKETYTIVIPPP
                     NVTGKLHLGHAWDTTLQDIITRMKRMQGYDTLYLPGMDHAGIATQAKVEAKLNEQGIT
                     RYDLGREKFLEQAWDWKEEYASFIRAQWAKLGLGLDYSRERFTLDEGLSKAVKKVFVD
                     LYNKGIIYRGERIINWDPKARTALSDIEVIHEDVQGAFYHFKYPYADGEGFIEIATTR
                     PETMLGDTAIVVNPNDERYKDVIGKTVILPIVGRELPILADEYVDIDFGSGAMKVTPA
                     HDPNDFEIGQRHQLENIIVMDENGKMNDKAGKYEGMDRFDCRKQLVKDLKEQDLVIKI
                     EDHVHSVGHSERSGAVVEPYLSTQWFVRMEDLAKRSLDNQKTDDRIDFYPQRFEHTFN
                     QWMENIRDWTISRQLWWGHQIPAWYHKETGEIYVGEEAPTDIENWQQDEDVLDTWFSS
                     ALWPFSTLGWPDLESEDFKRYYPTNALVTGYDIIFFWVARMIFQGLEFTDRRPFNDVL
                     LHGLVRAEDGRKMSKSLGNGVDPMDVIDEYGADSLRYFLATGSSPGHDLRYSTEKVES
                     VWNFINKIWNGARFSLMNIGEDFKVEDIDLSGNLSLADKWILTRLNETIATVTDLSDK
                     YEFGEVGRALYNFIWDDFCDWYIEMSKIPMNSNDEEQKQVTRSVLSYTLDNIMRMLHP
                     FMPFVTEKIWQSLPHEGDTIVKASWPEVRESLIFEESKQTMQQLVEIIKSVRQSRVEV
                     NTPLSKEIPILIQAKDKEIETTLSQNKDYLIKFCNPSTLNISTDVEIPEKAMTSVVIA
                     GKVVLPLEGLIDMDKEISRLEKELAKLQSELDRVDKKLSNENFVSKAPEKVINEEKRK
                     KQDYQEKYDGVKARIEQLKA"
     gene            9108..10379
                     /locus_tag="O2701_000976"
     CDS             9108..10379
                     /locus_tag="O2701_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCZ4292881.1"
                     /translation="MNYLESLYWIHERTKFGIKPGVKRMEWMLAQFNNPQNNIKGIHV
                     GGTNGKGSTVAYLRTALVENGYEVGTFTSPFIETFNERISLNGVPISNDAIVELVSRI
                     KPVSEMMERETDLGVATEFEIITAMMFLYFGEIHPVDFVIVEAGLGIKNDSTNVFTPV
                     LSILTSIGLDHTDILGGTYLDIARDKGAIIKPNVPVIYAVKNEDALKYVRERAIEQHA
                     KPIELDREIVVVSQNDEFTYRYKDYELETIILSMLGEHQKQNAALAITALIELNEQGL
                     IELDFNKMIDGIESVRWTGRIEQVHDKPLIILDGAHNSESIDALIDTIKQYHDKEKVD
                     ILFSAINGKPINEMVKHLSLIAHTFYATEFDFPKALRKEEIVGSIENDEIQLVDDYVE
                     FIKNYQGDTLVITGSLYFISEVKSKLDFEAL"
     gene            10649..11356
                     /locus_tag="O2701_000977"
     CDS             10649..11356
                     /locus_tag="O2701_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCZ4292882.1"
                     /translation="MVVLLSYSCSCIFSFLYQFISIEETSFDYLHRRSKCDYCNSSLK
                     WYELMPIISFLLLKGRCRNCRKRISLTHFLGETFALIPIVFIKYDFTYVNATLFITTY
                     VFLLIFTMTDITSLMLDCRLIIIYCIVSLSLSMIYPVAFIIISMTTHIFYFLFRAYIG
                     YGDVLLISALSLFFPLQFTIYVILFTFVIAGLVALITMIFKPIKLLPLVPFIFISFFI
                     NSLFYNDIHQFLGGVYF"
     gene            11353..12039
                     /gene="radC"
                     /locus_tag="O2701_000978"
     CDS             11353..12039
                     /gene="radC"
                     /locus_tag="O2701_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCZ4292883.1"
                     /translation="MKIKEMVTSEMPRERLLSHGAKSLSNTELLAILINTGRKGFSSI
                     DISNELLKSASNLNELKKSSINDLIKVKGIGLQKAITLKAAFELGERMGRRAENNRIK
                     ITQPSDVADYMIPTMKDLTQEHFVILLLNSKNVVIKETCVFKGTLNSSIVHPREIFSI
                     AVRENANAIIAVHNHPSGDVTPSQEDIITTMRLKECGLILGIDLLDHIIIGDNRFTSL
                     VEAGYFDEND"
     gene            12107..12391
                     /locus_tag="O2701_000979"
     CDS             12107..12391
                     /locus_tag="O2701_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292884.1"
                     /translation="MTLFIIIGVLVPMVYTMQLNIKNEPVTKRNLLITLALSTLGILV
                     TALAGVIVTKQAFPLLSVAIGSIFTGIVWGLLLSGSYALIRFLSNAFGRK"
     gene            complement(12508..12705)
                     /locus_tag="O2701_000980"
     CDS             complement(12508..12705)
                     /locus_tag="O2701_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292885.1"
                     /translation="MSILTIILIALLVILLFRVGLSILRFLIYVGLVLLCIYLGYQGL
                     IWLLDFFQINSGFLPHFQFNN"
     gene            12865..13338
                     /locus_tag="O2701_000981"
     CDS             12865..13338
                     /locus_tag="O2701_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4930 family protein"
                     /protein_id="HCZ4292886.1"
                     /translation="MRLIFSMIKNIIAVIAIVIIVYIALKYAPFLKEQDWNPVNHDND
                     QMNQVTQPTNDAQHVYVSGEKYSLKENDLIKNVPLSQIKNVFKMIDKQEFMAVSGMNR
                     MAYNDQYIIGQRGDEFILYKFGDESMRVYNTEFEMQQDLNELGQNLQLKSENAYQ"
     gene            13412..13531
                     /locus_tag="O2701_000982"
     CDS             13412..13531
                     /locus_tag="O2701_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292887.1"
                     /translation="MAEQSKQKQANEQQKAQNLFARWRQLQNSNSESSNDTNK"
     gene            13731..14573
                     /gene="mreC"
                     /locus_tag="O2701_000983"
     CDS             13731..14573
                     /gene="mreC"
                     /locus_tag="O2701_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCZ4292888.1"
                     /translation="MLKFFKNNKLIVVLCAIIVFIALIGLSIRSQSQSPPEQYIGDSV
                     SFGQRVVSYPVNFVAGTIGDFFKKGDSKESKNKISQLESKNQQLEAENEKLKKELDLK
                     DISKFDPISTTVLARNPDQWMNTIVIDKGSKSGITSNMAVMTSQGFVGRVTKVNKFSS
                     QVDLISTNTRAGKLSVNIQHGSKNIFGLIDRYDEKNSELVISDINNRDNISKGDKVVT
                     SGLADQLPSNLYIGEVTKVQNDQYGLAKEVRVKTGADLTDLSHVYVAKRDPKTIPDDE
                     SRDK"
     gene            14573..15103
                     /gene="mreD"
                     /locus_tag="O2701_000984"
     CDS             14573..15103
                     /gene="mreD"
                     /locus_tag="O2701_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCZ4292889.1"
                     /translation="MRTLYYFLIGILLFYIDTAIGLLIPMHIGKFELVFVPHLTFMYI
                     LMMVVYRGFGVSLLLSIFLGVMTDVYFGSIYGVYLFGYILFLALIDRFFKIFYKDHSM
                     LFIIILASTLLLEVYVALIYGMLGFIQFDIIHFVVFRLLPTLIMNFVLLIMLYPLIIK
                     FLKKTNNDIDMKRRQW"
     misc_feature    15130..15257
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            15270..15578
                     /gene="rplU"
                     /locus_tag="O2701_000985"
     CDS             15270..15578
                     /gene="rplU"
                     /locus_tag="O2701_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCZ4292890.1"
                     /translation="MFAIIETGGKQIKVEEGQEIFVEKLDVNEGDTFTFDKVLFVGGD
                     SVKVGAPTVEGATVTATVNKQGRGKKITVFTYKRRKNSKRKKGHRQPYTKLTIDKINA
                     "
     gene            15584..15904
                     /locus_tag="O2701_000986"
     CDS             15584..15904
                     /locus_tag="O2701_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HCZ4292891.1"
                     /translation="MITVDITVNDEGKVTDVIMDGHADHGEYGHDIVCAGASAVLFGS
                     VNAIIGLTSERPDINYDDNGGHFHIRSVDTNNDEAQLILQTMLVSLQTIEEEYNENIR
                     LNYK"
     gene            15916..16200
                     /gene="rpmA"
                     /locus_tag="O2701_000987"
     CDS             15916..16200
                     /gene="rpmA"
                     /locus_tag="O2701_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCZ4292892.1"
                     /translation="MLKLNLQFFASKKGVSSTKNGRDSESKRLGAKRADGQFVTGGSI
                     LYRQRGTKIYPGENVGRGGDDTLFAKIDGVVKFERKGRDKKQVSVYAVAE"
     gene            16555..17847
                     /gene="obgE"
                     /locus_tag="O2701_000988"
     CDS             16555..17847
                     /gene="obgE"
                     /locus_tag="O2701_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HCZ4292893.1"
                     /translation="MFVDQVKISLKAGDGGNGITAYRREKYVPFGGPAGGDGGKGASV
                     VFEVDEGLRTLLDFRYQRHFKASKGENGQSSNMHGKNAEDLVLKVPPGTIIKNVETDE
                     VLADLVEDGQRAVVAKGGRGGRGNSRFATPRNPAPDFSEKGEPGEELDVSLELKLLAD
                     VGLVGFPSVGKSTLLSIVSKAKPKIGAYHFTTIKPNLGVVSTPDQRSFVMADLPGLIE
                     GASDGVGLGHQFLRHVERTKVIVHMIDMSGSEGREPIEDYKVINQELAAYEQRLEDRP
                     QIVVANKMDLPESQDNLNLFKEEIGEDVPVIPVSTITRDNIDQLLYAIADKLEEYKDV
                     DFTVEEEESVGINRVLYKHTPSQDKFTISRDDDGAYVVSGNAIERMFKMTDFNSDPAV
                     RRFARQMRSMGIDDALRERGCKNGDIVRILGGEFEFVE"
     gene            17860..18315
                     /locus_tag="O2701_000989"
     CDS             17860..18315
                     /locus_tag="O2701_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HCZ4292894.1"
                     /translation="MDNKDYKKFYLIREDVLPESVVKTLKIKDALKSDPTLSIYDAVK
                     QFDLSRSAFYKYRETIFPVDDKMLDHREFTLILYVTDIVGMLARVLDVISKLELSVLT
                     IHQSIPMEEKATITLSLNAKSKETSVEDVIGALRNLDYVSKVELISMSM"
     gene            18329..18931
                     /gene="ruvA"
                     /locus_tag="O2701_000990"
     CDS             18329..18931
                     /gene="ruvA"
                     /locus_tag="O2701_000990"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCZ4292895.1"
                     /translation="MYAYVKGKLTHLYPTHVVVETAGVGYEIQTPNSYRFQKHLDHEV
                     LIHTSLIVREDAQLLYGFSSEEEKDMFLSLIKVTGIGPKSALAILATSTPNEVKRAIE
                     NENDTYLTKFPGIGKKTARQIVLDLKGKVKITEEDSDSLLQVDATSTVQDQFVQEAML
                     ALEALGYSKRELAKVEKTLNKNKYDSVDEAVKAGLQLVVS"
     gene            18963..19967
                     /gene="ruvB"
                     /locus_tag="O2701_000991"
     CDS             18963..19967
                     /gene="ruvB"
                     /locus_tag="O2701_000991"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCZ4292896.1"
                     /translation="MNERMVDQSMHSEETDFELSLRPTRLRQYIGQNSIKSNLEVFIK
                     AAKLRHEPLDHVLLFGPPGLGKTTLSNIIANEMEVNIRTVSGPSLERPGDLAAILSGL
                     QPGDVLFIDEIHRLSSVVEEVLYPAMEDFFLDIIIGKGDEARSIRIDLPPFTLVGATT
                     RAGSLTGPLRDRFGVHLRLEYYNESDLKEIIIRTAEVLGTGIDEESAIELAKRSRGTP
                     RVANRLLKRVRDFQQVNEDEQIYIETTKHALGLLQVDQHGLDYIDHKMMNCIIKQYNG
                     GPVGLDTIAVTIGEERITIEDVYEPFLIQKGFLERTPRGRKATPLAYEHFAKSNEERE
                     "
     gene            19969..20994
                     /gene="queA"
                     /locus_tag="O2701_000992"
     CDS             19969..20994
                     /gene="queA"
                     /locus_tag="O2701_000992"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCZ4292897.1"
                     /translation="MNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFK
                     DIIEYFRPGDTLVLNDTRVMPARLFGLKEETGAKVEMLMLTQIEGNDWEVLLKPAKRI
                     KVGNKLNFGNGKIIAECIKEMDQGGRIMRLHYEGILQERLDELGEMPLPPYIKERLDD
                     PDRYQTVYAKESGSAAAPTAGLHFTDELLIEIKNKGVNIAFVTLHVGLGTFRPVSVDD
                     VNDHEMHSEYYQMTQETADLLNDTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGW
                     TNIFIYPGFDFKAIDGQITNFHLPKSTLVMLVSAFSSRENVLNAYKTAVNLEYRFFSF
                     GDAMLII"
     gene            21017..22156
                     /gene="tgt"
                     /locus_tag="O2701_000993"
     CDS             21017..22156
                     /gene="tgt"
                     /locus_tag="O2701_000993"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016425271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCZ4292898.1"
                     /translation="MPAVTYEHIKTCKQSGARLGIVHTPHGSFETPMFMPVGTKATVK
                     TMSPEELRQIEAKIILGNTYHLWLQPGNDIIKHAGGLHKFMNWDGPILTDSGGFQVFS
                     LSNLRKITEEGVEFRHHTNGSKLFLSPEKSMQIQNDLGSDIMMAFDECPPMPAEYDYV
                     KKSIERTTRWAKRCLDAHQRPEDQALFGIIQGGEYEDLREQSAKDLVELDFPGYAIGG
                     LSVGEPKPVMYKMVEHTEQFMPKDKPRYLMGVGSPDALIECSIRGMDMFDCVLPTRIA
                     RNGTCMTSQGRLVIKNAKFADDLRPLDENCDCYTCQNYSRAYIRHLIKAEETFGIRLT
                     TIHNLHFLLKLMEDIRQAIREDRLLDFKEEFFEQYGLNVENPKNF"
     gene            22175..22435
                     /gene="yajC"
                     /locus_tag="O2701_000994"
     CDS             22175..22435
                     /gene="yajC"
                     /locus_tag="O2701_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCZ4292899.1"
                     /translation="MQFSLLIYIVVIFAVMYFLMIRPQQKRAKQHRELINNIQSGQRI
                     TTIGGIKGTVKAVDETTVVITVNGHGTELTFEKPAIKQVDPS"
     gene            22710..24989
                     /gene="secDF"
                     /locus_tag="O2701_000995"
     CDS             22710..24989
                     /gene="secDF"
                     /locus_tag="O2701_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HCZ4292900.1"
                     /translation="MKKSSRIIAFLLLVVLLFAGMAATYKSVIKNVNLGLDLQGGFEV
                     LYQVDPLNKGDKIDKKALQSTAQTLENRVNVLGVSEPKIQVEEPNRIRVQLAGVTDQN
                     EARKILSSQANLTIRDAEDKVKLSGSDIKQGSAKQEFKQETNQPTVTFKVKDKNKFKK
                     VTEEISKKRDNVMVVWLDFKKGDSYKKEAQKKNPKFISAASVDQPINSDSVEISGGFK
                     GQEGVKKAKQIAELLNAGSLPVDLKEIYSNSVGAQFGQDALDKTVFASFIGVALIYLF
                     MLGFYRLPGLVAIIALTTYIYLTLVAFNFISGVLTLPGLAALVLGVGMAVDANIIMYE
                     RIKDELRIGRTIKQAFSKANKSSFLTIFDSNLTTVIAAAVLFFFGESSVKGFATMLLL
                     GILMIFVTAVFLSRFLLSLLVSSNIFKNQFWLFGVKKNKRHDINEGVDVHDLKTSFEK
                     WNFVKLAKPLIGVSILIVVVGLVILYIFKLNLGIDFSSGTRVDFQSKQAITQQKVEQV
                     VKDSGLKADQIQINGKDNKVATVQFKDDLTRAQDNKLSDNIKSKFGDTPQINTVSPII
                     GQELAKNAMLALIYASIGIIIYVSLRFEWRMGLSSVLALLHDVFIIVAIFSLFRIEVD
                     LTFIAAVLTIVGYSINDTIVTFDRVRENLQKVKVITTTEQIDDIVNRSIRQTMTRSIN
                     TVLTVIVVVVAILFFGAPTIFNFTLALFIGLISGVFSSIFIAVPLWGIMKKRQLKKSP
                     KHKLVVYKEKKSNDEKILV"
     gene            25192..27465
                     /gene="recJ"
                     /locus_tag="O2701_000996"
     CDS             25192..27465
                     /gene="recJ"
                     /locus_tag="O2701_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCZ4292901.1"
                     /translation="MIKPKYKWKLTKPAEYISDELTSKLKLTPIVKKILESKSIIDEQ
                     AIESIISDTDINHDALQLSDMTKTIERIKRAIANDEKILVYGDYDADGVTATTILVIT
                     LQLLGAQVGWHIPNRFTEGYGPNELAFRNAHDEGITLIITVDNGIQGHNEIKMVQDLG
                     VDVIVTDHHEIGSTLPEAYAIVHSMHPSFNYPFQQLCGAGVAYKLAQALIENVPDYFK
                     ALVAIGTIADLVSLTDENRSLVKQGLKVLNDQCPTSVKALLKEAGYNDNIDEETIGFI
                     IGPRLNAVGRLDDASLACELLMTDVEEEAAFLAEQVEHFNRERKDIVATITEEAMAMA
                     ETKVKKGDLFLLLAKENWHEGVLGIVASKIVETFALPTLILNIDREQNHAKGSARSID
                     QVSMFEILSAHQELIAKFGGHHMAAGMTMDIENIESLAEGLNKWMKELSKTTSLDPVK
                     QVDVLLTENDITIKNIRDMNRLRPFGTDFSRPIFEMDDLSVSSVKAIGQQKNHLKLTL
                     GESNIAALFWQNGHLEPELQDEQPINILGSVQINEWNGNQSPQIIIQDIAMNEQQILD
                     YRSKRKSLPFTENDENIVVLIHPKSDKVNANEYYYGEEIKQQTDKVVLRDLPTSMEDL
                     SNSLQQLQFSQLYIVLQHNHSIYFDGIPNMDIFKKCYKALITKQETNIQKEGMLLCQH
                     LSVKPDTLKFMLKVFLDLKFVTQEDGLIRINQQPDKRSIDSSKVYQLRQQRMDVEKQL
                     LYQDFSEIKNWIKSQLS"
     gene            27487..28005
                     /locus_tag="O2701_000997"
     CDS             27487..28005
                     /locus_tag="O2701_000997"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009559512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCZ4292902.1"
                     /translation="MDLKQYVSEVQDWPKPGVSFKDITTIMDNGEAYGYATDKIVEYA
                     KDRDVDIVVGPEARGFIIGCPVAYSMGIGFAPVRKEGKLPREVIRYEYDLEYGTNVLT
                     MHKDAIKPGQRVLITDDLLATGGTIEAAIKLVEKLGGIVVGIAFIIELKYLNGIEKIK
                     DYDVMSLISYDE"
BASE COUNT         9906 a         3837 c         5366 g         8932 t
ORIGIN      
        1 atatgattaa tttatttttt gcaacacatt ttgtgaattg agaaaataat ctagagcaat
       61 ccgttcctag gaggaattga acatgcgtaa attagtcgtt ggctccagaa gaagtaaatt
      121 agctttaaca caaagccagc aatttattga taaattaaaa gctgtcgagc caaatctaga
      181 aattgaaatt aaagaaattg tcacgaaagg cgatcgtata gtagataaac aattgtctaa
      241 agtcggaggc aaaggcttat ttgttaaaga aatacaacat gaactttttg aaaaaaatat
      301 cgatatggca atacactcgc ttaaagacgt accaagtgta attccggaag gtttaacatt
      361 aggttgtatc cctgatagag aattaccttt tgatgcgtat atttctaaaa cacatacacc
      421 actatcccaa ttgccagaag gcagtattat tggtactagt tcattacgtc gtggtgcaca
      481 aatattatct aagtatccta atttagagat taaatggatt agaggtaata tagatacacg
      541 attagaaaag ttacaaactg aagattatga tgcgattatt ttagctgcag ctggtttaag
      601 aagaatgggc tggtcagatg atattgtaac atcttatctt gatagagata cattgttacc
      661 tgcaatcgga caaggtgctt tagggataga atgtcgtagt gacgatgaag aactattaac
      721 attattaagc aaagtacata atgatgaggt tgcaaaatgt gtgactgctg aacgaacgtt
      781 tttagcagaa atggatggta gttgtcaggt gccaattgca ggatatgcta caatctcaga
      841 tcaaaacgaa atcgaattta caggtttaat tatgacccca gatggtaaag aacgatttga
      901 atatacaatg aacggaacag atccggttga gttaggcaaa acagtgagta acaaattaaa
      961 agagcaaggt gcttatgaaa ttataaaacg cttaaatgaa caacattaaa taatggagtg
     1021 acgtttaaat atgaagccag ttgtagttat gacacaaaca aatgacatgc aaagtgattt
     1081 agtatcaatt attcacaaac cctttattga tataaagcca ctaaattttg atattcattt
     1141 gttaaatcaa cgctatgact ggcttatttt ttcatctaaa aatgctgtga aattctttta
     1201 taaatatcta aaaggaatta atgttgataa cattgctgtg ataggaagta agacagcgca
     1261 atattgtgaa tcacttggca ttcgagttga ttttatgcca aacgactttt ctcaagaagg
     1321 atttttaaaa tcatttaatc aaactaacca aaaaatactt ttgccttcga gtgaattggc
     1381 gagaccattg ttattagcag cgttatctaa agataatgaa gttgttaaaa tagatttata
     1441 tacttcagtg cctaacaaac aaaatataca agatgttaaa gaaatgatag aacatcaaca
     1501 aatcgatgca ttaacatttt caagttcgtc ggcagtacgt tattatttta atgaaggatt
     1561 tgtaccaaaa ttcaagtcgt attttgctat tggagaacaa acagcacgga ccattaaatc
     1621 atatcaacaa ccagtaacaa ttgcagaaat tcaaacactc gaatcactaa ttgaaaagat
     1681 tttagaaagt aggggctaaa aatgaaattt gatagacata gaagattgag atcatcagcg
     1741 acaatgagag atatggttag agagaatcat gtaagaaaag aagatttaat atatccaatt
     1801 tttgtagttg aaaaagacga tgtgaaaaaa gaaattaagt cattgccagg tgtataccaa
     1861 atcagtttga atttacttga aagtgaatta aaagaagctt atgacttagg catacgtgcc
     1921 attatgtttt tcggtgttcc aaactcaaaa gatgatatag gtactggtgc atacattcac
     1981 gatggtgtta ttcaacaggc aacacgtatt gctaaaaaaa tgtatgatga cttattaatt
     2041 gttgcagaca cttgtttatg tgaatatact gatcatggtc attgtggcgt gattgatgac
     2101 catacacatg acgttgacaa tgataaatca ttgccactac ttgttaaaac agcaatttct
     2161 caagtggaag ctggtgctga tattattgcg ccaagtaata tgatggatgg ttttgttgct
     2221 gaaattcgtc gtggattaga tgaagccggc tattacaata ttcctataat gagttatggt
     2281 gtcaagtatg catcaagttt ctttggacct tttagagatg cagcagattc agcgccatca
     2341 tttggggata gaaaaacgta tcagatggac cctgctaacc gtttggaagc acttcgtgaa
     2401 ttagaaagtg atcttaaaga agggtgcgac atgatgattg ttaaacctgc tctaagttat
     2461 ttagatatag ttcgagatgt taaaaatcat acgaatgttc cagttgttgc atataatgtg
     2521 agtggagaat atagtatgac taaagcagcg gcacaaaatg gttggataga tgaagaacgt
     2581 gtcgttatgg aacaaatggt ttcaatgaaa cgtgcaggtg ctgatatgat tattacgtat
     2641 tttgcaaagg acatttgtcg ctatttagat aaataaggtt ttatatttat gattttccat
     2701 aaactgtagg aggaatttac tttatgagat atacgaaatc agaagaagca atgaaggttg
     2761 ctgaaacttt aatgcctggt ggtgtaaata gtccagtacg cgcatttaaa tcagtagata
     2821 caccagcaat ttttatggat cacggtaaag gttcaaaaat ttatgatatc gatggtaacg
     2881 agtatatcga ctatgtacta agttggggac cacttatttt aggacataga gaccctcaag
     2941 ttattagtca tttacatgaa gcaattgata aaggtacaag ttttggtgca tcaacattac
     3001 ttgaaaataa attggcgcag ctcgttattg accgagtacc ttcaatagaa aaagtgcgta
     3061 tggtgtcatc tggtacagaa gctacattgg atactttaag attagcacgt ggttatactg
     3121 gcagaaataa aattgtgaaa tttgaaggtt gctatcatgg tcatagtgat tcgttattaa
     3181 tcaaagctgg ttctggggtg gcaacattag gattgccgga ttctcctggt gtgcctgaag
     3241 gtattgctaa aaatacaatt acagttccat acaatgattt agatgcactt aaaatcgctt
     3301 tcgaaaaatt tggaaacgat attgctggtg taatcgtaga acctgttgct ggtaatatgg
     3361 gtgtcgtacc gccgattgaa ggttttttac agggattaag agatattacg actgaatacg
     3421 gcgcattgct aattttcgat gaagtaatga ctggtttcag agtcggttat cattgtgcac
     3481 aaggttactt tggtgtgaca ccagatttaa cttgcttagg aaaagttatc ggtggaggac
     3541 tacctgtagg tgcatttggt ggtaaaaaag aaatcatgga tcatatagca ccattaggaa
     3601 atatttatca agcgggtacg ttatcaggaa atcctcttgc aatgacaagt ggttatgaaa
     3661 cgttaagcca attaacgcca gagacatatg agtattttaa tatgttaggc gatatacttg
     3721 aagacggttt aaaacgtgta tttgctaaac acaatgtacc aataactgta aatagagcag
     3781 gttcaatgat tggttatttc ttaaatgaag gacctgtaac taattttgaa caagcgaata
     3841 aaagtgattt gaaattattt gcagaaatgt atcgagaaat ggcaaaagaa ggtgtgtttt
     3901 taccaccatc tcaatttgaa ggtacattct tatctacggc acacacgaaa gaagatattg
     3961 aaaaaacgat tcaagcattt gatacggctt taagtcgtat tgtaaaataa atatacggac
     4021 aaattgagag cctgaacttt gttcaggctc tttttaaatg tatataaggc atgggcggcg
     4081 acttgatagt gaaagtccat atatgctttg tagtcaaaac tgctagcgga tattgttatc
     4141 ttaacaaacg tgaagctcaa gcagcaattt agtcatttta ttttttattg aaagaagtgg
     4201 aaaacatggc aatgatatat agaaataatt tcattgtgtt cgttttatca ttttttatta
     4261 gtattctact gtattcatcg cacgtattac ttccatttat gtttggtcct attatcgcat
     4321 caatcatttg tgtaaaagtt ttcaaacttg atattaaatg gccattctta cttagtgaat
     4381 taggtattgt actattaggt gtgcaaatcg gatcaacgtt tacgaaaaat gtcgttatgg
     4441 atattaaaaa caattggctt tcgattattg ttgtatctat ttcgatatta ttaattgcaa
     4501 tagtaatggc atttgttttt aaaaaaattg cacgtattaa tacagaaaca gctattttaa
     4561 gtgttatacc aggagcacta acacaaatgc tggtcatggc tgaacaagac aaacgtgcta
     4621 atttgttagt tgttagctta acgcaaacat cacgaattat atttgttgtt gttttagtac
     4681 cgttcatttc atattttttt catgatggta acatgcatgc gaatggaaag ttaacaaaag
     4741 tcttgccttt atcacaagta ttaaacatag ggcaaatagt tattttagcg atagctatct
     4801 ttatagttta tctaattatg tctaaaataa agtttccaac atttcaatta ttagcaccac
     4861 tcattgtatt aattgtttgg aatttttcta caggtttaac atttacacta gatcattggt
     4921 tgttgaacat ggcacaacta atatatatga ttagaattgg agttcaaatt gcgcatttat
     4981 tgtcagattt aaaaggtaga ctagcaatcg caattacaat tcaaaatatt atgttgataa
     5041 ttggtgcgct aatcatggtt tatatcatac atttctttga taataatcct attaatgaat
     5101 tgtttcttgg tgcagcacca ggaggggtta atcagatagt tttagttgca ttagctactg
     5161 gtgccgatgt tgctatgatt acgagttatc atattttccg aatctttttc atcctattta
     5221 ttattgcacc aggaattaat tactttttaa aatatcgttc gaataagcgt gaagattaga
     5281 aactatcatc catctaagtt gaattatgct taggatagaa ttcattatta aggtttaagc
     5341 aaaggttgta catctatttt tagattaaga tatctaatat tagcagagca ttaacttgga
     5401 actttaactt atgatttgaa taaattttca cattaaaaaa cgagacagtc tattagttaa
     5461 aaatagatga tgtctcgttt actttatgat gctatagcaa attaattatg tttaggcttt
     5521 gatggacaat cttgtaaatg tgcatcatat aaaccggctg cttctaaaaa cgaaaatact
     5581 gttactggac ctaaaaattt aaacccgtat tgttttaaat ctttagatag ttgtgttgca
     5641 gtatcatcaa ctgtgatacg atcagaagca tgttcatact gcaaatcttt aggcttacca
     5701 tttacatatg accataaaaa tttactaaaa ctaccatatg cttgttcaat ttttaaatac
     5761 ccttgagctt gattaacaat tgcttctaat tttttacgat gatgaacgat atttggaaaa
     5821 gtcattaagc ggtcgatatc ttgagcggtc atttgtgcta ccttttctgg ttcgaaatca
     5881 taaaatgctt cttcataggc ttctttcttt tttaaaatag ttaaccaaga tagcccagca
     5941 tgttgtgatt ctaatgctaa aagtttaaac aatgccttgc tatcatagag cggttgtccc
     6001 catacatgat catgatagtc taagtagact ggatctttag taccaaatgc gcattcattc
     6061 attttattcc cacctataca ttgacttatt ttgtcaatca ttatactata agttaagagt
     6121 ttaatatagg atttgggaag agtaagttca aattatttta aagagagcat atgatttggt
     6181 gtgagtatgt aaatgatttt tactgaaggt agcccacaga gtatcttagc tgaacgtgat
     6241 taatagtagg ctaagacgtg tttgagcgtt aatcataatc tagagtgctt gaagagatga
     6301 agcatttcaa atttcacgat gatagtattg gtatgattat tccaattatg ttaaaagagt
     6361 gaatttgata ttaatcatcc ttcttgaata taggtggtac cacggaatat ccgtcctatt
     6421 tgtatatagg atggatattt ttattttttt aggaggtata gcaaatggaa atgaaaccaa
     6481 aatatgatcc tcgtgaagtt gaagcgggac gttatgaaga atgggtaaag aatggttatt
     6541 ttaaaccgtc agaagataaa tcaaaagaaa catatacaat tgttatcccg ccaccaaatg
     6601 taactggtaa attacattta ggacatgcat gggatacgac tttacaagat atcattacac
     6661 gtatgaaacg tatgcaagga tacgatacgt tatacttacc aggtatggat catgctggta
     6721 ttgcgacaca ggcaaaggta gaagctaaat taaatgaaca aggaataact agatatgatc
     6781 ttggtcgtga aaagttttta gaacaggcat gggattggaa agaagagtat gcgtcattta
     6841 ttcgtgcgca atgggctaaa ttaggtctag gtttagatta tagtagagaa cgttttactt
     6901 tagatgaagg tttaagtaaa gcagttaaaa aagtttttgt tgatttatac aataaaggaa
     6961 ttatttatcg tggcgaacgt attataaatt gggatcctaa agcacgtaca gctttatctg
     7021 atattgaagt aatacatgaa gatgttcaag gtgcgtttta tcattttaaa tatccttacg
     7081 ctgatggtga aggttttatt gaaattgcaa caacaagacc agaaacgatg ttaggtgata
     7141 cagcgattgt tgttaaccct aatgacgaac gatacaaaga tgtaatcggt aaaactgtta
     7201 tattaccaat cgtaggacgc gaactgccta ttttagcaga tgagtatgtt gatatagact
     7261 tcggttctgg tgctatgaaa gtgacaccag cacatgaccc taatgatttt gaaattggtc
     7321 aaagacatca attagaaaat attatcgtta tggatgaaaa tggtaaaatg aacgacaaag
     7381 cgggtaaata tgaaggtatg gaccgttttg attgtcgtaa acagctagtt aaagatttaa
     7441 aagaacaaga tttagttatc aagattgaag atcatgttca ttctgtaggt cattcagaac
     7501 gatctggcgc tgttgttgaa ccatatttat caacacaatg gtttgtgcgc atggaagact
     7561 tagcgaaacg ttcattagat aaccaaaaaa cagatgatcg tattgatttt tatccgcaac
     7621 gtttcgaaca tacatttaac caatggatgg aaaatattag agattggacg atttcaagac
     7681 aattatggtg gggtcatcaa attccggctt ggtatcataa agaaacaggc gaaatatatg
     7741 ttggagaaga agcgccaact gatattgaaa attggcaaca agatgaagat gtattagata
     7801 cgtggttctc aagtgcttta tggcctttct ctacgttagg ttggcctgat ttagaaagtg
     7861 aagactttaa acgatactac ccaacaaatg ccttagttac aggttacgat attatctttt
     7921 tctgggtagc acgcatgata ttccaaggct tagaatttac agatcgtcgt ccatttaatg
     7981 atgtattatt acacggttta gttcgtgctg aagacgggcg taagatgagt aaatcattag
     8041 gtaatggtgt ggatccaatg gatgttattg acgaatacgg tgctgatagc ttgcgttact
     8101 tcttagcaac aggttcatct ccaggacatg atttaagata ctcaactgaa aaagttgagt
     8161 cagtgtggaa ctttatcaat aaaatctgga atggggcacg tttcagttta atgaatatcg
     8221 gtgaagactt taaagttgaa gatatcgatt taagtggtaa cttatcatta gcagataaat
     8281 ggattctaac acgtttaaat gaaacgattg caacagttac tgatttaagt gacaaatatg
     8341 aattcggcga agttggacgt gcattatata atttcatttg ggatgatttc tgtgattggt
     8401 acattgaaat gagtaaaatt ccaatgaata gtaatgatga agaacaaaaa caagttacac
     8461 gttcagtatt gagttatact ttagacaata ttatgagaat gctacatcca ttcatgccat
     8521 ttgtaacaga gaaaatatgg caaagtttac cacatgaagg tgacacaatt gttaaagctt
     8581 catggccaga agtgcgtgaa tcattgattt ttgaagaaag taaacaaaca atgcaacaac
     8641 ttgttgaaat cattaaatct gtaagacaat cacgtgtaga agtaaatacg ccattgtcta
     8701 aagaaatacc tattttaatt caagctaaag ataaagaaat tgaaacaact ttatcacaaa
     8761 acaaagatta tttaatcaaa ttctgtaatc ctagtacctt aaatattagc actgacgtgg
     8821 aaattcctga gaaagcaatg acatcagttg taattgcagg taaagtggta ttaccattag
     8881 aagggctaat tgatatggat aaggaaatca gccgtttgga aaaagaatta gctaaacttc
     8941 aaagcgaatt agatagagta gataaaaagc tctctaatga aaactttgta agtaaagcac
     9001 ctgaaaaggt tataaatgaa gaaaaacgta aaaaacaaga ttatcaagaa aaatatgatg
     9061 gtgtgaaggc aagaattgaa caattaaaag cataggagtt agtaacaatg aattacctag
     9121 agagcttgta ttggatacac gaaagaacta aatttggcat caaaccaggt gttaaacgta
     9181 tggaatggat gctagcacaa tttaataatc ctcaaaataa cattaagggt attcatgtag
     9241 gtggcacaaa tggtaaaggc tctacagttg cttaccttag aacagcttta gttgaaaatg
     9301 gttatgaagt aggtacattt acgtcgccgt ttattgaaac atttaatgaa cgaattagtc
     9361 taaatggtgt gccaatatca aatgacgcta ttgtagaatt agtatcacgt attaaaccag
     9421 taagtgaaat gatggaacgt gaaacagatt taggtgttgc aactgaattc gaaataatca
     9481 cagcgatgat gtttttatat tttggtgaaa tacatcctgt tgattttgtc attgttgagg
     9541 ctggattggg tataaagaac gattcgacaa atgtctttac accggtttta tcaatcttaa
     9601 ctagtatcgg tctagaccat acagatattt taggtggtac ttatctagat attgctaggg
     9661 ataaaggcgc gattataaag cctaacgttc cagtgatata tgctgttaaa aatgaagatg
     9721 cattaaaata tgttcgtgaa cgcgcaattg aacaacatgc aaagccaatt gaattagata
     9781 gagaaattgt tgttgtatcg caaaatgatg aatttactta ccgttataaa gattatgaat
     9841 tagaaacaat cattttaagc atgttaggtg aacatcagaa acaaaatgct gcattagcca
     9901 taacagctct tattgaatta aatgaacaag gattaattga attagatttc aataagatga
     9961 tagacggtat tgaatcagtt cgttggactg gacgtattga gcaggtgcat gacaaacctt
    10021 taatcatttt ggatggcgca cataattcag agagtataga tgctctaatt gatacaatta
    10081 aacagtacca tgataaagaa aaagtagata ttttgttctc agcaataaac ggaaaaccga
    10141 ttaacgagat ggtcaaacat ttaagtttaa ttgcgcatac gttttatgca actgaatttg
    10201 attttccgaa agcgttacgc aaagaagaaa ttgtaggtag tattgaaaat gatgaaatac
    10261 aattagtaga tgactacgtt gaatttataa aaaattatca aggtgataca ttagtaatta
    10321 ccggtagtct gtatttcata agtgaagtta aatcaaaatt agactttgaa gcattataaa
    10381 actaaaacta aaatagccat gaagaatcta tttaatcaat aggtagagct tcatggttat
    10441 tttttatgct ctaaatcgta tgtgtttata aggttcaatt tcttgccatt attaattttg
    10501 ccgagcagta atgggttgtt aaagtatcca tcaattatgc ttagcaatat tgaaaaattt
    10561 aaatgaaaaa gcggttcaat tcttgaaaaa tagtgtatat ttacattttg gtaataacta
    10621 actataattt acataaggag gttgtcattt ggtagtattg ttatcttata gttgcagttg
    10681 tatttttagt tttctatatc aatttatatc tatagaagaa acgtcatttg attatttaca
    10741 tagaagatcg aaatgtgatt attgtaattc gtcactcaaa tggtatgaat taatgccgat
    10801 tattagtttt ttattattaa aagggcgatg tcgaaactgt cgaaagcgta tttccctaac
    10861 acatttctta ggggaaacct ttgctttaat acctatcgtc tttattaagt atgatttcac
    10921 atacgtaaat gctacgctat ttataactac atatgttttt ctgcttattt ttactatgac
    10981 cgatatcact tctttaatgt tagattgtcg cttaattata atttattgta tcgtttctct
    11041 ctcgttaagt atgatttatc cagtagcttt tatcattatt agtatgacca cgcatatatt
    11101 ctacttttta tttcgggcat atattggtta tggtgacgtt ttactaatat ctgcactttc
    11161 tttgtttttc cctctccaat tcactattta tgtcatttta tttacatttg tcattgctgg
    11221 tttagttgct ttaattacca tgatatttaa gccgattaaa ctattacccc ttgttccatt
    11281 tatatttatt tcctttttta tcaattcact tttttataat gatatccatc aatttttagg
    11341 aggcgtatat ttttgaaaat taaagaaatg gtaacttcag aaatgccaag agaacgtttg
    11401 ttaagccatg gtgcaaaaag tctttcgaat acagaattat tagctatatt aattaacacc
    11461 ggaagaaaag gattctcgag catagacatt agtaatgaac tgcttaaatc tgcttcgaat
    11521 ctgaatgaat tgaaaaaatc ttcaattaac gatttgataa aagttaaagg gattggatta
    11581 caaaaagcaa ttactttaaa agcagcattt gagttagggg aaaggatggg gagaagagct
    11641 gaaaataatc gtataaaaat aacgcaacca agtgatgttg ctgattatat gattccaaca
    11701 atgaaagatt taacacaaga acattttgtc attttattgt taaattcaaa aaatgtagtg
    11761 attaaagaaa cctgtgtttt taaaggtaca ttaaatagtt cgattgtaca tccacgtgaa
    11821 atttttagta ttgcggtgag agaaaatgcc aatgcaatca tcgcagttca taatcatcca
    11881 tccggtgatg taacgccctc acaagaagat atcataacaa caatgaggtt gaaggagtgt
    11941 ggtttgattt tagggataga tttattggat catattataa tcggtgataa tagatttacc
    12001 agtcttgtag aagcgggtta ctttgatgaa aatgattgaa gttgatagaa ttaatgacgt
    12061 atcttgtgta taatacctac gaagtacttt cattggagga aaaatagtga ctctatttat
    12121 tattatcggg gttctcgtgc caatggttta taccatgcag ttaaatatta aaaatgaacc
    12181 tgtaacaaag cgcaatcttt taataacatt agctttatct acgttaggta ttttagtaac
    12241 cgcgttagca ggtgtaatcg ttacgaaaca agcttttcct ttattaagtg tagcaattgg
    12301 ctcaattttt actggaatcg tttggggcct tttactaagt ggtagctacg cgctgatacg
    12361 atttttatct aacgcatttg ggcgtaagta atagtaatct gttcatcaag tagtatccgt
    12421 gcttgaaaac aaactaaaac tcctaatgtg gaactagtta aaaaattcta aactagtaca
    12481 ttaggagttt tgttatgcag aataaattta attgttaaat tgaaagtgcg gtaaaaatcc
    12541 actatttatt tgaaaaaaat cgagcaacca aattaaacct tgatatccta agtaaataca
    12601 taataaaaca agtccgacat aaattaaaaa tcgcaaaata gaaagtccaa ctctaaaaag
    12661 gaggatgact aatagtgcta ttaaaataat tgttagtata ctcaatgcta caaacctcct
    12721 aatacgcttt taaatcataa caaaattata agctatttta aatgaacgtg catcagtcct
    12781 aagatatatc atgattaagg ctgaagactg aggaaaatat acataataaa atatataatt
    12841 agatgaagac attggaggcg aactatgaga ttgatattta gtatgattaa aaacattata
    12901 gcagtaatag caattgtgat aattgtttat atcgccttga aatacgcacc ttttttaaaa
    12961 gaacaagatt ggaatcctgt aaatcacgat aatgatcaaa tgaatcaagt tacgcaaccc
    13021 actaatgatg cccaacatgt ttatgtaagt ggtgagaaat attcattaaa agaaaatgat
    13081 ttaataaaga atgtaccatt aagccaaatt aaaaatgtat ttaaaatgat agataaacaa
    13141 gagtttatgg ctgtgtctgg aatgaatcgc atggcttata atgatcaata tattataggt
    13201 caaagaggag acgaatttat tctttataaa tttggagatg agtcaatgcg tgtttacaat
    13261 actgaatttg aaatgcaaca agacttaaat gaattagggc aaaatttaca actaaaatct
    13321 gaaaatgctt atcaatagga aattattgca taagaaatat attttagtta tcctgtcact
    13381 agaataaatt taaaaggttg gtgtagttca aatggcagaa caatcaaaac agaaacaagc
    13441 taatgaacaa caaaaggccc agaacttatt cgcacgttgg agacaacttc agaattcgaa
    13501 cagtgaatcc tcgaacgata caaacaaata attttaaaat ttaaatcgat tatttaaact
    13561 actagtgact ggaatgtttg ctagtagttt ttttgttttt aaaatgccac aaatttttta
    13621 cggagttgtt cattttcaaa gtaattcgta gttagtgctt tatttcataa tgggagtaat
    13681 ataaaataga aaaggatata caatattttg acataataga ggtgttctgg gtgcttaagt
    13741 tttttaaaaa taacaaatta attgttgttt tatgtgcaat tatcgttttt attgcattaa
    13801 ttgggctgtc catacgttca caatctcaat cacctcctga acaatatata ggtgattctg
    13861 tgtcttttgg acaacgagtt gtgagttatc cagttaattt tgttgctggt acgattgggg
    13921 acttttttaa aaaaggagat tctaaagaat ctaaaaataa gattagccag ttagaatcta
    13981 agaaccaaca attagaagcg gaaaatgaaa aattaaaaaa agagcttgat ttaaaagata
    14041 tttcaaaatt tgatcctatt tctactacgg ttttggcaag aaatccggat cagtggatga
    14101 atacaattgt aattgataag ggatctaaat caggtataac ttcaaatatg gctgtgatga
    14161 catcacaagg ttttgttgga agagttacta aagttaataa attttcttca caagttgatt
    14221 taatctcaac taatacacgt gcgggtaaat tatctgtaaa tatacaacac ggttctaaaa
    14281 atatatttgg tttaattgat cgttatgatg aaaagaactc agaacttgta attagtgaca
    14341 ttaataatag agataatatc tcaaaaggtg ataaagtcgt tacaagtgga ttagctgatc
    14401 aactaccaag taatttatat ataggagaag tgactaaggt tcaaaatgat caatacggct
    14461 tagctaaaga agttagggtt aagactggtg cggacttaac agatttgagt catgtttatg
    14521 ttgcaaaaag agatcctaaa acaattcctg atgatgaaag cagggataaa taatgcgtac
    14581 actgtattat tttttgatag gtattttact attttatata gatactgcaa ttgggcttct
    14641 tattccaatg cacataggta agtttgaact tgtatttgta ccacacctta catttatgta
    14701 tattttaatg atggtagtgt atcgtggctt tggcgtatca ttattgctca gcatattttt
    14761 aggcgtaatg actgatgtat actttggtag tatttatgga gtgtacttat ttggctatat
    14821 attgttttta gcacttatag atcgattctt taaaattttt tacaaagatc attcgatgtt
    14881 attcattatt atattagcca gcaccttatt attagaagtc tatgtggcat taatatacgg
    14941 tatgttagga ttcattcaat ttgatattat tcattttgta gtctttagat tattgccaac
    15001 attaattatg aattttgtac tgttgattat gctttatccg ttgattataa agttccttaa
    15061 aaaaacaaac aatgacattg acatgaaacg tcgtcaatgg taagatgcaa tagttgagta
    15121 gttataagta gctacataca accgctcaaa tataggttca agaacatttt taatgaagaa
    15181 agcgattttc ccacagagtg caaacgcttg actcaataac gaaatgtcac ctatgtatgg
    15241 cgtgacttat tatacaggag gtgcaaagta tgtttgctat tattgaaaca ggtggaaaac
    15301 aaatcaaagt agaagaaggt caagaaatct tcgttgaaaa attagacgta aacgaaggag
    15361 atacttttac atttgataaa gtattatttg taggtggaga ttcagttaaa gttggagcgc
    15421 caacagttga aggtgcaaca gttactgcta ctgttaataa acaaggtcgc ggtaaaaaaa
    15481 tcactgtatt cacatacaaa cgtcgtaaaa attcaaaacg taaaaaaggc catcgtcaac
    15541 catacactaa attaacaatc gataaaatca acgcgtaatt attatgatta ctgttgatat
    15601 tacagttaat gatgaaggca aagtaacaga cgttattatg gatggccatg ctgaccatgg
    15661 tgaatatggt catgatatcg tttgtgctgg agcttcagct gtattgtttg gtagtgttaa
    15721 tgcgattata ggattgacat ctgagagacc agatatcaat tatgacgaca atggtggtca
    15781 ttttcatata agaagcgttg atacaaacaa cgatgaagcg caactaattc ttcaaacaat
    15841 gcttgtgtct ttacaaacta ttgaagaaga atataatgag aatattagat taaattataa
    15901 gtgaggtgca ttccgatgtt aaaattaaac ttacaattct tcgcatctaa aaaaggggta
    15961 agttctacaa aaaacggacg tgactctgaa tcaaaacgct taggtgctaa acgtgctgac
    16021 ggtcaattcg taacaggtgg ttcaatttta tatcgccaac gtggtactaa aatttaccct
    16081 ggtgaaaatg taggtcgtgg tggcgatgat acattattcg ctaaaatcga cggcgttgtt
    16141 aaattcgaac gtaaaggtcg cgacaaaaaa caagtttctg tatatgcagt agctgaataa
    16201 ttttgtctag ttaacaccag aagtgaatct tctggtgttt tttacttttt ataaaatgtt
    16261 tttcattatt attttcatta tgttattaaa aaatgggaat tttagacgtt atactaaatg
    16321 tgcaactgta taagagcccc taatcactaa actataaggg ggacaaagga atacagttgc
    16381 agcgtttaaa gaataaactg taccacaatt ggtgctgaga aatataagta ttttaaagca
    16441 aagatttata aaagtaactg cataagagcc cctaattatt tacaatatat aaggggctct
    16501 aatatgctat aattattggg aaaatgaaaa ttatatgtaa aagaggtgag atatatgttt
    16561 gtcgatcaag tcaaaatatc tcttaaagcc ggtgatggtg gtaatggtat taccgcatac
    16621 agaagagaaa aatatgtacc atttggtgga ccagctggcg gtgacggtgg taaaggtgct
    16681 tcagtcgtat ttgaagtgga tgaaggttta agaacgttat tagattttag atatcaacgt
    16741 cattttaaag caagcaaagg tgaaaatggc caaagtagta atatgcatgg taaaaatgcg
    16801 gaagatttag tattaaaagt tccacctggt acaattatta aaaatgttga aacagacgaa
    16861 gtgttagcag atcttgttga agatggtcaa agagctgtag tagcgaaggg cggtcgaggt
    16921 ggccgaggta attcacgttt tgcaacacct agaaaccctg cacctgactt cagtgaaaaa
    16981 ggtgaaccag gtgaggaatt agatgtatct ttagaattga aattattagc tgatgtagga
    17041 ttagtaggtt tccctagtgt gggtaaatcg actttattat ctatcgtttc aaaagctaag
    17101 cctaaaattg gggcatatca ttttacaacg attaaaccaa atctaggtgt tgtttcaacg
    17161 cctgatcaac gtagttttgt tatggcagat ttaccaggtt taattgaagg tgcatctgat
    17221 ggcgttggat taggacatca atttttaaga catgtagaga gaacaaaagt tattgttcac
    17281 atgattgata tgagcggttc tgaaggtaga gaacctattg aagattataa agtcattaat
    17341 caagaattag ctgcgtacga gcaacgttta gaagatagac ctcaaatcgt agtagctaac
    17401 aagatggatt tacctgaatc acaagataat ttaaacttgt ttaaagaaga aattggcgaa
    17461 gatgtgccag ttattccagt ttcaacaata acgcgtgata atattgatca attattatat
    17521 gcaatagcag ataaattaga agaatataaa gatgttgact tcacagttga agaagaggag
    17581 tcagttggca ttaaccgagt attatataaa catacaccgt cacaagataa atttacaatt
    17641 tcaagagatg atgatggtgc ttatgtggta agtggtaatg ctattgaaag aatgtttaaa
    17701 atgactgact ttaacagtga tccagcagta cgtcgatttg ctcgtcaaat gcgttcgatg
    17761 ggtattgatg atgcgcttag agaacgtggt tgtaaaaatg gtgatatcgt tagaattctt
    17821 ggcggagaat ttgaattcgt tgaataggag cgaaacatga tggacaataa agattataaa
    17881 aagttttatt taattagaga agatgtcttg cctgaatccg tggttaaaac attgaagatt
    17941 aaagatgcct taaaaagtga tccgacattg tccatttatg atgccgttaa acagtttgat
    18001 ctatctagaa gtgcttttta taaatataga gaaacgatat ttccagtaga cgataaaatg
    18061 cttgaccatc gagaatttac attaatttta tatgtaactg atattgttgg tatgttggca
    18121 cgtgtactag atgttatatc aaagttagaa ctatctgtat taacgattca tcaaagtatt
    18181 ccaatggaag aaaaagcaac aataacatta tcactgaatg ctaaatctaa agaaacttca
    18241 gtagaagatg ttattggcgc tttgagaaat ttagattatg tatcaaaagt agaattaatt
    18301 agtatgagta tgtaaggatg tgcctataat gtacgcgtat gtcaaaggta agttaacaca
    18361 tttatatcct acacacgtag ttgttgaaac tgctggtgtt ggttatgaaa ttcaaacacc
    18421 aaattcttat cgttttcaaa agcatctaga tcatgaagtt ttaattcata catctttaat
    18481 tgttcgtgaa gatgcacaat tattgtatgg atttagtagt gaagaagaga aagatatgtt
    18541 cttgagttta attaaagtta ctggtattgg tccgaaatca gctttagcta ttttagcgac
    18601 aagtacgcct aatgaagtaa aacgtgccat tgaaaatgaa aatgatacgt atttaactaa
    18661 attcccagga attggtaaga aaacggcaag acagattgtc ttagatttaa aaggtaaagt
    18721 gaaaattact gaagaagata gcgattcatt attacaagta gacgctactt cgacggtgca
    18781 agatcaattc gtgcaagaag caatgttagc gttagaagca ttaggttatt ctaaacgaga
    18841 gcttgcaaaa gttgagaaaa cgttaaataa aaataaatat gactcagttg atgaagctgt
    18901 taaggcaggt cttcaattag ttgtatctta attttaaata gattaatagg ggaagtgttg
    18961 tcatgaatga gcgtatggtt gatcaatcaa tgcatagtga agaaactgat ttcgaattgt
    19021 cgcttagacc tacgagatta cgacaatata ttggtcaaaa ttcaataaaa agtaatttag
    19081 aagtatttat taaagcggct aaacttcgtc atgaaccatt agatcatgta ttgctttttg
    19141 gcccccctgg attaggtaag acaacattat ctaatatcat tgccaatgaa atggaagtta
    19201 atatacgtac agtatcaggg ccttcattag aaagacctgg tgatttggct gcaattttat
    19261 caggacttca acctggagat gttttgttta ttgatgaaat acacagactg agtagtgttg
    19321 ttgaagaagt gttataccct gcaatggaag atttcttttt agatattatc attggtaaag
    19381 gcgatgaggc tagaagtatc cgtatcgact tacctccatt cactttggta ggtgcaacaa
    19441 cgcgagctgg cagcttaaca ggtccactaa gggatcgatt tggtgtgcac ttaagattag
    19501 aatattataa cgaatcagat ttaaaagaaa tcattattag aacagctgag gttttaggca
    19561 caggtattga tgaagaaagt gccattgaac ttgctaaacg ttctagaggg actccaagag
    19621 tagcaaatcg actattgaag cgggtaagag acttccagca agtgaatgaa gatgaacaaa
    19681 tatacattga aacaacgaag cacgcattag gtttacttca agttgatcaa cacggactag
    19741 attacattga tcataaaatg atgaactgta ttattaagca gtataatggc ggacctgttg
    19801 gtttagatac gattgccgta acaattggtg aagaacgtat tacaattgag gacgtttatg
    19861 agccatttct tattcagaaa ggctttttag aacgtacgcc acgtggcaga aaagcaacac
    19921 cattagctta tgaacatttt gcaaagtcga atgaggagag agaataacgt gaatattgaa
    19981 gaatttgact atgacttacc agaatcatta attgctcaaa cgcctttaaa agatcgtgat
    20041 catagtcgtt tattagtcat ggatagagaa actggtgaaa tgaaacattt acatttcaaa
    20101 gatatcattg agtattttag acctggtgat acattagtgc ttaacgatac gcgagtaatg
    20161 ccagctagac tttttggttt aaaagaagaa actggtgcaa aagttgaaat gttaatgtta
    20221 actcaaattg aaggtaatga ttgggaagtc ttactgaaac cagctaagcg tattaaagtt
    20281 ggtaataaat tgaattttgg taatggcaaa attatagctg aatgcataaa agaaatggat
    20341 caaggtggac gcatcatgcg tttacattat gaaggtattt tacaagaaag attagatgaa
    20401 ttaggggaaa tgccactgcc accatacatc aaagaacgtt tagatgatcc agatcgttat
    20461 caaacagttt acgctaaaga aagtggttca gcggcagcac caacagcagg attacatttt
    20521 actgatgagt tattaattga aattaaaaat aaaggtgtta atatcgcatt tgttacatta
    20581 catgttgggt taggtacgtt tagaccggtg agcgtagacg atgtgaatga ccacgaaatg
    20641 catagtgaat attatcaaat gacacaagaa acagctgatt tattaaatga tactaagtcc
    20701 aaaggacatc gcattatatc agttggtaca acttcaacac gtacacttga aacaattcga
    20761 cgcgatcatg ataaatttgt tgaaacgagt ggctggacta atatatttat ttatccagga
    20821 tttgatttta aagcaattga tggccagatt actaattttc atttaccaaa atcaacatta
    20881 gttatgctag tatcagcgtt tagtagtcgt gaaaatgttc tgaatgctta taaaacggca
    20941 gtaaatttag aatatagatt ctttagtttt ggcgatgcaa tgttaattat ataaaaagaa
    21001 tgtgaggatt ttgaatatgc ctgcagtaac atacgaacac attaaaactt gtaaacaatc
    21061 aggtgcgcgt ttaggtatcg tgcacacacc acacggttca tttgaaacac ctatgtttat
    21121 gccagttggt actaaagcaa ccgttaaaac aatgagtcca gaagagttaa gacaaattga
    21181 agcaaaaatc attttgggca acacatatca tttgtggtta caacccggaa atgatattat
    21241 caaacacgct gggggattac ataaattcat gaattgggat ggtccgattc ttacagattc
    21301 aggcggtttc caagtgttta gtttaagtaa tttacgtaaa attacagaag aaggcgtgga
    21361 atttagacat catactaatg ggtctaaatt atttttgagt cctgagaaat caatgcaaat
    21421 tcaaaatgat ttaggatctg atattatgat ggcatttgat gaatgtccac cgatgcctgc
    21481 tgaatatgat tatgtaaaaa aatctattga acgtacaaca cgttgggcga aaagatgtct
    21541 agatgcacac caaagacctg aagatcaagc attgttcggc attatacaag gtggcgaata
    21601 tgaagattta agagaacaaa gtgcaaagga tttagtagaa ttagattttc ctggttatgc
    21661 aatcggcggt ttgtcagttg gtgaacctaa accggttatg tataaaatgg ttgaacatac
    21721 agagcagttt atgcctaaag ataaaccaag atatttaatg ggtgtaggat ctccagatgc
    21781 gttaatcgaa tgtagtattc gcggcatgga tatgtttgat tgtgtcttac cgacacgtat
    21841 tgccagaaat ggtacttgta tgacatcgca aggtcgttta gttattaaaa atgcaaaatt
    21901 tgcagatgat ttaagaccgt tagatgagaa ttgtgactgt tatacatgtc aaaactattc
    21961 aagagcgtat atacgtcatt taatcaaggc agaggaaact tttggtattc gtcttactac
    22021 tattcataat ttacattttc tgctaaaatt aatggaagat ataagacaag ccattcgaga
    22081 agatcgtctt ttagatttca aagaagaatt cttcgagcaa tatggattaa atgttgagaa
    22141 cccaaaaaac ttttaagcaa gaggagcgta taaaatgcaa ttttcattac taatatatat
    22201 agtcgtaatt tttgcggtta tgtatttctt gatgatcaga ccacaacaaa aacgtgcgaa
    22261 acagcatcgt gagttgatta ataacattca atctggtcaa agaattacaa ctattggtgg
    22321 tattaaaggt actgttaaag cagtagatga aacaactgtt gttattacag ttaatggtca
    22381 tggtactgaa ttaactttcg aaaaacctgc tattaaacaa gttgaccctt cataatgaga
    22441 agtggtatta aaaaggatga aacgaatagc gtttcatcct ttttaagtta taagtagtat
    22501 gtagaatagc ttatttttat tccaaaaaaa gaaattgtga tacataataa ttgttaaatt
    22561 taaattaaat atatctatga ctatttttac tccctgctct agtatgttaa tatgtataag
    22621 gctaataaat tttgtaagaa agatttaact tgcgttataa aatagtattc aagtattcta
    22681 aatacatacg taaataatga ggtgttcatg tgaagaaaag tagtagaata attgcgttct
    22741 tgcttttagt cgtattgttg tttgccggaa tggctgcaac ttataaaagt gttataaaga
    22801 atgtcaattt aggacttgat ttgcaaggtg gatttgaggt gctttatcaa gtcgatcctt
    22861 taaataaagg cgacaaaata gataaaaaag ccttacaatc aacagcgcaa acattagaaa
    22921 atcgtgttaa cgttttaggt gtttcggaac ctaaaattca agttgaagaa cctaatagaa
    22981 ttagagtaca acttgctggt gtaacagacc agaatgaagc acgtaaaatt ttatcttctc
    23041 aagctaattt aacaattcgc gatgcagaag ataaagtaaa attaagcggt tctgatatta
    23101 aacagggatc tgccaaacaa gaatttaaac aggaaacaaa ccagcctaca gttactttta
    23161 aagtaaaaga caaaaacaaa tttaaaaaag taacggaaga aatctctaag aaacgtgata
    23221 atgtcatggt tgtttggtta gatttcaaaa aaggtgacag ctataaaaaa gaagcacaaa
    23281 agaaaaaccc taaatttatt tctgcagcaa gtgttgatca accaatcaat tctgatagcg
    23341 tagaaatctc gggtggattt aaaggacaag aaggcgttaa aaaagcaaaa caaatcgccg
    23401 aattgttaaa tgcaggttct ttaccagttg acttgaaaga aatttattca aactcagtag
    23461 gtgcccaatt tggtcaagat gcattagata aaactgtgtt tgcatcgttt attggtgtag
    23521 cattaattta tttattcatg cttggattct accgattacc tggtttagta gcgattattg
    23581 cattgacaac ttatatctat ctaacgttag tagcatttaa tttcatttcc ggggttctaa
    23641 ctttaccagg attagcggcg ttggtattag gtgtaggtat ggctgtagat gccaatatta
    23701 tcatgtatga gcgtattaag gatgaacttc gaataggtag aacgataaag caagcctttt
    23761 ctaaagcaaa caaaagttca ttcctaacaa tttttgattc taacttaaca acagttatcg
    23821 ccgcagcagt attattcttc ttcggtgaaa gttcagttaa aggtttcgcg acaatgttat
    23881 tattaggtat tctaatgatc tttgttacag ccgtgttctt atcaagattc ttattatcat
    23941 tacttgtttc atcaaatata ttcaaaaatc aattttggtt atttggtgtt aaaaagaata
    24001 aacgacatga tattaatgaa ggtgtagatg ttcatgacct taaaacttca ttcgagaaat
    24061 ggaattttgt taaattagct aagccattaa ttggagtaag tattttaatt gtagttgtcg
    24121 gtttagttat tctttatatc ttcaagttaa acttaggaat tgatttctca agtggtacac
    24181 gcgtagattt ccaatctaaa caagcaatta ctcaacaaaa ggtagagcaa gttgtaaaag
    24241 atagtggact taaagcggat caaattcaga tcaatggtaa agacaataaa gtagcaactg
    24301 ttcaatttaa agacgattta acgcgtgctc aagataataa attgagtgat aatattaaat
    24361 cgaaattcgg tgacacacct caaattaaca ctgtatcacc aattattgga caggaattag
    24421 caaaaaatgc aatgcttgca ttaatctatg catcgatagg tatcatcatc tatgtatcat
    24481 tacgatttga atggcgcatg ggtctttcat ctgtattggc attattacat gatgtattca
    24541 ttatagtagc gattttcagt ttatttagaa ttgaagtaga tttaacattt atcgccgctg
    24601 tattaacaat tgtcggttat tcaattaatg atacaatcgt aacgtttgac cgtgtacgtg
    24661 aaaacttaca aaaggttaaa gtgattacga caacagaaca aattgatgat atcgttaata
    24721 gatcaattag acagacaatg acacgttcaa ttaatacagt attaacagtt attgtagtag
    24781 tagttgctat actattcttc ggtgctccta cgatattcaa ctttacttta gcattattta
    24841 tcggattgat ttctggtgta ttctcttcaa tcttcattgc cgttccgcta tggggaataa
    24901 tgaaaaaacg tcagttgaaa aaatcgccga aacacaaatt agttgtatat aaagaaaaga
    24961 aatcgaacga tgaaaagatt ttagtttaaa atgaattaag cggtatgtga aacaataaag
    25021 agttcacata ccgcttttta ctttatttaa atttaacaaa atatatataa ataagttccc
    25081 gcattcattt ttaaaaaatg gtataattaa agtcttgatt atttagataa gtgcttttta
    25141 tttaaagtaa ttttagatat aatgtctttt ggaaatgagg cgtaaatata tatgattaaa
    25201 ccgaagtata agtggaaatt aactaaacca gctgaataca taagtgatga attaacatca
    25261 aaattaaagt taacgccaat cgtaaaaaaa atattagaaa gtaaatcgat tattgatgaa
    25321 caagcaatag aatccatcat ttcagataca gatatcaatc atgatgcact gcaattgagc
    25381 gatatgacta agactattga aagaattaaa agagcgatag cgaatgatga gaaaatccta
    25441 gtttatggtg attacgatgc cgacggtgtt accgcaacta caattttagt gataacatta
    25501 caacttttag gtgcacaagt aggatggcat attcctaacc gctttaccga aggatatgga
    25561 ccaaacgaac tagcatttag aaatgctcat gatgaaggca taacattaat cataactgtt
    25621 gataatggca tacaaggtca taatgaaatt aaaatggttc aagatttagg agttgatgtc
    25681 attgtaactg atcatcatga aattggtagt acattaccag aagcatatgc aatcgtacat
    25741 tcaatgcatc catcgtttaa ttatccgttc caacaattgt gtggtgcagg tgttgcatac
    25801 aaattagcac aagcattgat tgaaaatgta cctgattatt ttaaagcatt agttgcgata
    25861 ggtaccattg cggatttagt atccttaact gatgaaaatc gatcattagt gaaacaagga
    25921 ttaaaagtct taaatgatca atgtccgacc tctgttaaag cattgctaaa agaagcggga
    25981 tacaatgaca atattgatga agaaacaatt ggttttatta ttggacctag acttaatgct
    26041 gtaggtcgtt tagatgacgc atcattagct tgcgaattgt tgatgactga cgtcgaagaa
    26101 gaagctgcat ttttagcaga acaagttgaa cattttaacc gtgaaagaaa agatattgta
    26161 gcaactatca cagaagaagc aatggctatg gcagaaacga aagttaaaaa gggagattta
    26221 tttttacttt tagcaaaaga aaattggcat gaaggtgtct taggtattgt tgcttcgaaa
    26281 attgtcgaaa catttgcttt gccaacatta attttaaata ttgataggga gcaaaatcat
    26341 gcaaaaggct ccgccagatc aattgatcaa gtgtcgatgt ttgaaatact atcagcacat
    26401 caagagttaa tcgctaaatt tggcggccat catatggctg caggtatgac gatggatatt
    26461 gaaaatatag aatcgttagc tgaaggttta aataaatgga tgaaagaatt atctaaaaca
    26521 acttcgttag atcccgtaaa gcaagtagat gtattattaa cagaaaatga tataacgatt
    26581 aaaaatattc gtgatatgaa tcgtttaaga cccttcggaa cagatttttc tagacctatt
    26641 tttgaaatgg atgatttatc agtttcttca gttaaggcga taggtcaaca aaaaaatcat
    26701 ctaaaattga cacttggtga aagtaatatc gccgcgctat tttggcaaaa tggacactta
    26761 gaacctgagt tacaagatga acagccaatt aatatattag gatctgttca aatcaacgaa
    26821 tggaatggta atcaatcacc gcaaataatt attcaagata ttgcgatgaa tgaacagcaa
    26881 atattagatt atagaagtaa gcgaaaaagt ttacctttta cagaaaatga tgaaaatatt
    26941 gtcgtgctta ttcatcctaa aagtgataaa gtaaatgcga atgaatatta ttatggtgaa
    27001 gaaattaaac aacaaactga taaagtagta ttaagagatt taccaacgtc aatggaagac
    27061 ttgtctaatt ccttgcaaca actgcaattt tctcaacttt atatagtttt gcaacataat
    27121 cattcgattt acttcgatgg tatacctaat atggatattt ttaaaaagtg ttataaagca
    27181 ttaataacta aacaagaaac aaatatccag aaagagggta tgttattgtg tcaacattta
    27241 agtgtgaaac cagatacact taaattcatg ttgaaagttt tcttagactt aaaatttgta
    27301 acacaagaag atggtttaat tcgaataaat caacaacctg ataaaagatc gattgattcc
    27361 agcaaagtat atcaattaag acaacaacgt atggatgttg aaaagcaatt attatatcaa
    27421 gatttttcag aaataaaaaa ttggataaag tcacaattgt cgtgagcaat ttaggaggaa
    27481 atattaatgg atttaaagca atacgtatca gaagttcaag attggccgaa accaggtgtt
    27541 agtttcaagg atattactac aattatggat aatggtgaag catatggcta tgcaacagat
    27601 aaaattgtag aatacgcaaa agacagagat gttgatatcg ttgtaggacc tgaagcgcgt
    27661 ggctttatca ttggctgtcc tgtagcttat tcaatgggga ttggctttgc acctgttaga
    27721 aaagaaggga aattacctcg tgaagtcatt cgttatgagt atgacctaga atatggtaca
    27781 aatgttttaa caatgcacaa agatgcaatt aaaccaggtc aacgtgtgtt aattacagat
    27841 gatttattag ctactggtgg tacgattgaa gcagcaataa aattagttga aaaattaggc
    27901 ggtatcgtag taggtattgc atttataatt gaattgaaat atttaaatgg tattgaaaaa
    27961 attaaagatt acgatgttat gagtttaatc tcatacgacg aataataaat aatataattt
    28021 tatcaaatga aatccttcat c
//
LOCUS       DALAGD010000029        27952 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.029, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000029 DALAGD010000000
VERSION     DALAGD010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 27952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27952
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.029"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(458..904)
                     /gene="perR"
                     /locus_tag="O2701_000998"
     CDS             complement(458..904)
                     /gene="perR"
                     /locus_tag="O2701_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HCZ4292903.1"
                     /translation="MSVEIESIEHELEESIASLRQAGVRITPQRQAILRYLISSHTHP
                     TADEIYQALSPDFPNISVATIYNNLRVFKDIGIVKELTYGDSSSRFDFNTHNHYHIIC
                     EQCGKIVDFQYPQLNEIERLAQHMTDFDVTHHRMEIYGVCKECQDK"
     gene            complement(1001..1951)
                     /locus_tag="O2701_000999"
     CDS             complement(1001..1951)
                     /locus_tag="O2701_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCZ4292904.1"
                     /translation="MKVVGLNRMREVETELQQRFSDLDFKFYKKASEIPESDLADLDI
                     LVGYDGGINEAFLRRCPNLKWIAWFATGVNTLPLDYIADHGILLTNGKGVQAKQLSEY
                     ILAFILDDYKKMKLSYDNQRQHIYDSKITGKRLSGQTVLFLGTGAIATRTAKLAKAFN
                     MNLIGLSKSGQNKDEFDEIYTIESLESTLPNADIIINALPETQETIHLLKKKHFELMK
                     DEALFINIGRGSIVKEALLIEVLKSKVIRHAYLDVFENEPLKPNHELYELDNVTITAH
                     ITGNDYEAKYDLLDIFKNNLVNFLNKNGLIENEVDAKKGY"
     gene            complement(1957..2412)
                     /gene="bcp"
                     /locus_tag="O2701_001000"
     CDS             complement(1957..2412)
                     /gene="bcp"
                     /locus_tag="O2701_001000"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCZ4292905.1"
                     /translation="MLQKGEQFPIFKLENQDGTVITNDTLKGKKAIIYFYPRDNTPTC
                     TTEACDFRDNLEMFNDLDVAVYGISGDSKKKHQNFIEKHGLNFDLLVDEDFKLAKETG
                     VYQLKKSFGKESMGIVRTTFIIDEQGKVLDVIEKVKVKTQIEELKNILG"
     gene            2493..3782
                     /locus_tag="O2701_001001"
     CDS             2493..3782
                     /locus_tag="O2701_001001"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCZ4292906.1"
                     /translation="MNFSESERLQQLSNEYILGGVNSPSRSYKAVGGGAPVVMKEGHG
                     AYLYDVDGNKFIDYLQAYGPIITGHAHPHITKAIQEQAAKGVLFGTPTELEIEFSKKL
                     RDAIPSLEKIRFVNSGTEAVMTTIRVARAYTKRNKIIKFAGSYHGHSDLVLVAAGSGP
                     SQLGSPDSAGVPESVAREVITVPFNDINAYKEAIEFWGDEIAAVLVEPIVGNFGMVMP
                     QPGFLEEVNEISHNNGTLVIYDEVITAFRFHYGAAQDLLGVIPDLTAFGKIVGGGLPI
                     GGYGGRQDIMEQVAPLGPAYQAGTMAGNPLSMKAGIALLEVLEQDGVYEKLDSLGQQL
                     EEGLLKLIEKHNITATINRIYGSLTLYFTDEKVTHYDQVEHSDGEAFGKFFKLMLNQG
                     INLAPSKFEAWFLTTEHTEEDIKQTLKAADYAFSQMK"
     gene            4075..5169
                     /locus_tag="O2701_001002"
     CDS             4075..5169
                     /locus_tag="O2701_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCZ4292907.1"
                     /translation="MRLGARIFKTGIAIILAMSIASLLPDDVGLKALAGVSAVVAMQP
                     SIYRSFKTVSDQALGNIIGAILSVTMVTIFSDNFIIMGVTVIVLIAILFKFNLAHVAT
                     LASVTALIIMGQHTGSFYITAFYRFVLVMIGVISSSLVNFVFLPPKFETKIYYNSLNI
                     SSDIFMWFKLVLNDTTEFNNIKQDSHNLKQRVEKLEKIYDYYSEERPITKKHIHQQNR
                     KKILFREVVQTTRQAYEVLNKLSRYQNDLYLLNNNFLLQIKLDLDSLTAFHEQILASL
                     SKKARYNVTHVDYELDNPQKKDLLSTFQHELINHPYQTEYSFANVMQIVAAIEEYRHH
                     LEHLDRIRISFFTYHRSDADIEIVEEDFDL"
     gene            complement(5259..5351)
                     /locus_tag="O2701_001003"
     CDS             complement(5259..5351)
                     /locus_tag="O2701_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292908.1"
                     /translation="MFDFSTILKRKFVTGWWGHNIEKAVRDFLN"
     gene            complement(5360..7096)
                     /locus_tag="O2701_001004"
     CDS             complement(5360..7096)
                     /locus_tag="O2701_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAV1866 family putative multidrug efflux ABC
                     transporter"
                     /protein_id="HCZ4292909.1"
                     /translation="MIKRYLQFVKPYKYRIFATIIVGIIKFGIPMLIPLLIKYAIDGV
                     INNHALTTDEKVHHLTIAIGIALFIFVIVRPPIEFIRQYLAQWTSNKILYDIRKKLYN
                     HLQALSARFYANNQVGQVISRVINDVEQTKDFILTGLMNIWLDCITIIIALSIMFFLD
                     VKLTLAALFIFPFYILTVYVFFGRLRKLTRERSQALAEVQGFLHERVQGISVVKSFAI
                     EDNEAKNFDKKNTNFLTRALKHTRWNAYSFAAINTVTDIGPIIVIGVGAYLAISGSIT
                     VGTLAAFVGYLELLFGPLRRLVASFTTLTQSFASMDRVFQLIDEDYDIKNGVGAQPIE
                     IKQGRIDIDHVSFQYNDNEAPILKDINLSIEKGETVAFVGMSGGGKSTLINLIPRFYD
                     VTSGQILIDGHNIKDFLTGSLRNQIGLVQQDNILFSDTVKENILLGRPTATDEEVVEA
                     AKMANAHDFIMNLPQGYDTEVGERGVKLSGGQKQRLSIARIFLNNPPILILDEATSAL
                     DLESESIIQEALDVLSKDRTTLIVAHRLSTITHADKIVVIENGHIVETGTHRELIAKQ
                     GAYEHLYSIQNL"
     gene            complement(7387..7929)
                     /locus_tag="O2701_001005"
     CDS             complement(7387..7929)
                     /locus_tag="O2701_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCZ4292910.1"
                     /translation="MVRESIPKEGENIKIQSYKHDGKIHRVWSETTILKGTDHVVIGG
                     NDHTLVTESDGRTWITREPAIVYFHSEYWFNVICMFREDGIYYYCNLSSPFVCDEEAL
                     KYIDYDLDIKVYPNGKYHLLDEDEYEQHMNQMNYPHDIDIILRRNVDILQQWIEQKKG
                     PFAPDFIKVWKERYKKIRQY"
     gene            complement(8231..9268)
                     /gene="mutY"
                     /locus_tag="O2701_001006"
     CDS             complement(8231..9268)
                     /gene="mutY"
                     /locus_tag="O2701_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCZ4292911.1"
                     /translation="MYQQSSFKENLIHWFDENQREMPWRQTTNPYYIWLSEVMLQQTQ
                     VKTVIDYYHRFVERFPTVEVLSQASEDEVLKYWEGLGYYSRARNFHTAIKEVHDKYEG
                     LVPKDPDQFKALKGVGPYTQAAVMSIAYNVPLATVDGNVFRVWSRLNDDYRDIKLQST
                     RKSYEQELLPYVTTEAGTFNQAMMELGALICTPKNPLCLFCPVQENCEAFDKGTFEKL
                     PVKSKNVSKKVIEQSVFLIRNNQGQYLLQKRSEKLLHGMWQFPMFESEHARRKMTEKI
                     GHDIQPVETPIFELKHQFTHLTWKIKVYAVSGAINIETLPDDMIWFDLSDRDQYTFPV
                     PMSKIYQFING"
     gene            9420..10397
                     /locus_tag="O2701_001007"
     CDS             9420..10397
                     /locus_tag="O2701_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCZ4292912.1"
                     /translation="MDTATHIAIGVGLTALATQDPAMASTFGATATTLIVGSLIPDGD
                     TVLKLKDNATYISHHRGITHSIPFTILWPILITFLIFTFFSGTNPFHVWMWAQLAVFL
                     HVFVDIFNSYGTQALRPITNKWIQLSVINTFDPIIFTVLCIGIVLWVIGLHPFAVFFP
                     IIALLIIYYMIRFKMRAVIKQQALKAIQQEHHPVKVFVAPTIKFMEWRVAIQTDAHDY
                     VGKAYGRNVVFSDKVERQTLSTDSILWKVKGNKDIRTFLNFSSIYRWQTTTLADGSTE
                     IRLIDLRYLKNDHYSFVAIAHVTNDNVIDHSYIGWVFTEDKLQRKLYAK"
     gene            complement(10658..11494)
                     /locus_tag="O2701_001008"
     CDS             complement(10658..11494)
                     /locus_tag="O2701_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292913.1"
                     /translation="MLDNIILYFKNLPHTKRYVTERLKQSWKSFLIVLAACLILIIAS
                     ETLFSFSHLTDVKEVRWLFRIIVLIVFAVVMFTIYISYHHYMNDFLVTKLFNISAATP
                     VVIMSILSFIMLVILTMISALVKPVNFETSYIALFYFIVMATIFVGLISVTFGLIRLL
                     TEKINIIFYGVCVLCFLLLPIIFIPNPNHVFINHILMLNPMYYIVNGIAQSIIFGISS
                     MENIPYHFYFILFLCLIAAVNFVLARYTTHAIYNKTSKVTQTDNQQDVSNDSTDEADT
                     SS"
     gene            complement(11503..13020)
                     /locus_tag="O2701_001009"
     CDS             complement(11503..13020)
                     /locus_tag="O2701_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4292914.1"
                     /translation="MGSSIVLKLLKVTHYYRNKQNKKWYLPFGYDAEDIDLNNISLHI
                     YQGEALGIIGEPESSKALVGQLLAGAIKPDKGKVVCTEDLFYGYIEDQSLIHQTVEAY
                     TAQLVQLFPYEINDHKAEQIIQYAHLGDYKTKPVNHISKAAYAQLLLSIARSSKSNII
                     ILNHVIDYLTPQFMERAIELTNDYIENNLTIVSIGDDIDKISQVSNYIAWFSHGQLRM
                     EGSLKQVIPSFKEHERDRLSLNSKEEIENFDLDWKKNRTRIPEMTYNFKRVERYNHAK
                     PPKFLVRFWTLASGTILGLALMMLLIFNNIGIISITDFTNRATMQNENKDPYGEKLAY
                     GIAFNGSVDMQGDKQVTIPKYSVVTITGENSKNYRVTADNKTYYVSKDKLEYFNPAGL
                     YQTHSFKKLAPYMKSNYSNYYAYFNSQLHKKHSSVIKTLVPDDDNRFVASVTQQPIQL
                     LFNDNNQLYGFVYPIVDKKELKDKFNINNNIWITKVGNGYCIANLKEDKWIYIEL"
     gene            complement(13035..13349)
                     /locus_tag="O2701_001010"
     CDS             complement(13035..13349)
                     /locus_tag="O2701_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhH family protein"
                     /protein_id="HCZ4292915.1"
                     /translation="MEQKKLSEMSEPELRHEIQLYKEKMRKAEMNGILNEYDVYQSKV
                     IVAESYLVDRKKIEIGKIYKLTDGSNQYFKVERLKGIFAWGFRFNSDEPEEGLPIALL
                     QL"
     gene            complement(13327..14145)
                     /gene="recX"
                     /locus_tag="O2701_001011"
     CDS             complement(13327..14145)
                     /gene="recX"
                     /locus_tag="O2701_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCZ4292916.1"
                     /translation="MPKITKIEVQKKNKERFNLFLDEQFEMGIDIDTLVKFNLKKGQQ
                     LEAADMAEIQKYDHYRIGLNKAIQYLSYKKRTEKEVIQYLQKEEISEQAISEVIEYCY
                     REKLIDHQDYAESLKNTMIRTTDKGPKIYQQKLYQLGIEPNIIEIFTELYREQQELDD
                     IIQIAEKISKTKKGPQNKVKEKVMQSLIQKGFEMETIHAVLNEMDFTQDEAVLDDLLQ
                     RDLEKIYNKNRKKYTQQKLISKTIEGLMRKGYKYDKIKAKLEESGIADGTEEIE"
     gene            complement(14406..15215)
                     /gene="sgtB"
                     /locus_tag="O2701_001012"
     CDS             complement(14406..15215)
                     /gene="sgtB"
                     /locus_tag="O2701_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional peptidoglycan glycosyltransferase
                     SgtB"
                     /protein_id="HCZ4292917.1"
                     /translation="MKRSDRYSNSNEHFEHMKHEPHYNTYYQPVGKPPKKKKSKRILL
                     KILLTILIIIALFIGIMYFLSTRDNVDELRKIENKSSFVSADNMPEYVKGAFISMEDE
                     RFYNHHGFDLKGTTRALFSTISDRDVQGGSTITQQVVKNYFYDNDRSFTRKVKELFVA
                     HRVEKQYNKNEILSFYLNNIYFGDNQYTLEGAANHYFGTTVNKNSTTMSHITVLQSAI
                     LASKVNAPSVYNINNMSENFTQRVSTNLEKMKQQNYINETQYQQAMSQLNR"
     gene            complement(15556..16071)
                     /locus_tag="O2701_001013"
     CDS             complement(15556..16071)
                     /locus_tag="O2701_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCZ4292918.1"
                     /translation="MTKKVAIILANEFEDIEYSSPKEALENAGFNTVVIGDTANSEVV
                     GKHGEKVTVDVGIAEAKPEDYDALLIPGGFSPDHLRGDTEGRYGTFAKYFTKNDVPTF
                     AICHGPQILIDTDDLKGRTLTAVLNVRKDLSNAGAHVVDESVVVDNNIVTSRVPDDLD
                     DFNREIVKQLQ"
     gene            complement(16202..16363)
                     /locus_tag="O2701_001014"
     CDS             complement(16202..16363)
                     /locus_tag="O2701_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292919.1"
                     /translation="MNEQQTIEQIKARLNKFIEDIDHVNPDEVRVEDIDEWIGLLDQL
                     EEKVKLVSK"
     gene            16587..17738
                     /gene="yfkAB"
                     /locus_tag="O2701_001015"
     CDS             16587..17738
                     /gene="yfkAB"
                     /locus_tag="O2701_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/CxCxxxxC motif protein YfkAB"
                     /protein_id="HCZ4292920.1"
                     /translation="MNSVKLKQPVSIYNDPWEAYNDVKEHGQLTLSNIEFTTTNLCNM
                     RCSHCAVGYTLQTVDPEPLDMDLIYRRLDEIPNLRTMSITGGEPMFSKKSIRNVVKPL
                     LKYAHHRGIYTQMNSNLTLPQDRYLDIAEYIDVMHISHNWGTTDEFANVGFGAMKKQP
                     PLKAKLKLYEQMISNARTLSEQGMFVSAETMLNQSTLPHLRKIHQEVVHDMKCSRHEI
                     HPMYPADFASQLNVLTLAEMKKTIHDILDFRDEDIWMLFGTLPVFPCLKDDEDQKLLS
                     RLRNAKNVTTRNDPDGRSRLNVNVFTGNVIVTDFGDETGTISNIQKDKLTDVFDKWLS
                     SDLAKSLNCHCSEFSCLGPNVLVKNMYYPNMDFKDNERHMHKQPQIIQF"
     gene            complement(17998..18528)
                     /locus_tag="O2701_001016"
     CDS             complement(17998..18528)
                     /locus_tag="O2701_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCZ4292921.1"
                     /translation="MTNQDRPMKSMSESKCYKNRQVFPQDTNHHHTMFGGTLMANIDE
                     IAAITAMKHAGAQVVTASTDSVDFLKPIKTGDILQYVAMVSYAGTSSMEVVVQIRIDD
                     VFNNKHDLAALSYLTFVALDDEGKPKHVPGVYPEDDVEKWFYDTAPQRVERRKARRIE
                     SKQTIEYLAQVQHIRD"
     gene            complement(18618..19874)
                     /locus_tag="O2701_001017"
     CDS             complement(18618..19874)
                     /locus_tag="O2701_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCZ4292922.1"
                     /translation="MGILTNYKEKLQQYAELLVKVGMNVQPKQPVFIRSSVETLELTH
                     LIVEEAYHCGASDVRVVYSDPTLKRLKFENESVEHFANHEIKSYDVEARMDYVKRGAA
                     NLALISEDPDLMDGIDSQKLQAFQQQNARAFKGYMESVQKNQFPWVVAAFPSKAWAKR
                     VYPELSVEEAYIKFIDEVFDIVRIDGNDPVENWRQHIANLSVYAQKLQQKNYHALHYV
                     SEGTDLTVGLAKNHIWEDATSYVNGKEQAFIANIPTEEVFTAPDRNRVDGYVTNKLPL
                     SYNGTIIDQFKLMFKDGEIIDFSAGKGEAVLKDLINTDEGSRRLGEVALVPDDSPISN
                     RNTIFYNTLFDENAACHLAIGSAYAFNIQGGTEMTVEEKIASGLNDSNVHVDFMIGSS
                     DLTIYGIFEDGSKELVFENGNWASTF"
     gene            complement(19877..20077)
                     /locus_tag="O2701_001018"
     CDS             complement(19877..20077)
                     /locus_tag="O2701_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1128 family protein"
                     /protein_id="HCZ4292923.1"
                     /translation="MTNEEKVLAIREKLNIVNQGLLDPEKYKNANEEELTDIYDFVQS
                     RERLSPSEVTAIADALGQLRHE"
     gene            20224..20688
                     /locus_tag="O2701_001019"
     CDS             20224..20688
                     /locus_tag="O2701_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HCZ4292924.1"
                     /translation="MVDVAFVCLGNICRSPMAEAIMRQRLKDRNIHDIKVHSRGTGSW
                     NLGEPPHEGTQKILNKHNIPFDGMISELFEATDDFDYIVAMDQSNVDNIKSINPNLKG
                     QLFKLLEFSNMEESDVPDPYYTNNFEGVYDMVLSSCDNLIDYIVKDANLKEG"
     gene            20695..20970
                     /locus_tag="O2701_001020"
     CDS             20695..20970
                     /locus_tag="O2701_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCZ4292925.1"
                     /translation="MENKFVPGILIGAVIGGAISLADKSTRQALVQSVKDAKNGNRTR
                     KPSKVSKIKDEVLYWKDVVEEIRRNNPELERSLKDAKETFVNRKNQR"
     gene            21248..22465
                     /locus_tag="O2701_001021"
     CDS             21248..22465
                     /locus_tag="O2701_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HCZ4292926.1"
                     /translation="MSKKDHSSSKYLNSVKEAQEESKKKNKSNPKIDVDRTYIEPQQF
                     QSKKPKKDDQVFFLSRLNKPAKYKKDSNFLSYLIYRIGKDDASGLAAQMTYHFVLAMF
                     PMLLFLLTLLPFFNIKQSQITNMLSNAPAETSTLIKSVIGDITQNSSGGLLSIGLILA
                     IWSASNGMTAIMNSFNVAYDVEDSRNGIVLKLLSVVFTVVMGVVFVVALALPTLGSVI
                     SHFLFGPLGFDEQVKWIFNLIRIVLPIIIIFIIFIVLYSVAPNVKTKLKSVLPGAVFT
                     SIIWLAGSFGFGWYISNFGNYSKTYGSIAGIIILLLWLYITSFIIIVGAEINAIIHQR
                     SVIKGKTPEEAALEHDDNNKNHYNENTRYEYDEDSNATHQRTYHVDEHPSDTYPEDDK
                     NIKDKIVDKLKKD"
     gene            complement(22581..23210)
                     /gene="vraR"
                     /locus_tag="O2701_001022"
     CDS             complement(22581..23210)
                     /gene="vraR"
                     /locus_tag="O2701_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator VraR"
                     /protein_id="HCZ4292927.1"
                     /translation="MTIKVLFVDDHEMVRIGISSYLSTQSDIEVVGEGASGKEAIAKA
                     HELKPDLILMDLLMDDMDGVEATTQIKKDLPQIKVLMLTSFIEDKEVYRALDAGVDSY
                     ILKTTSAKDIADAVRKTSRGESVFEPEVLVKMRNRMKKRAELYEMLTEREMEILLLIA
                     KGYSNQEIASASHITIKTVKTHVSNILSKLEVQDRTQAVIYAFQHNLIQ"
     gene            complement(23200..24243)
                     /locus_tag="O2701_001023"
     CDS             complement(23200..24243)
                     /locus_tag="O2701_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCZ4292928.1"
                     /translation="MNHYIRTIGSMLILVYSMLAAFLFIDKVFVNIIYFQGMFYTQIF
                     GIPVFLFLNLIIILLCIIVGSVLAYKINQQNDWIKTQIERSMEGETVGINDQNIEIYS
                     ETLDLYHTLVPLNQELHKLRLKTQNLTNENYNINDVKVKKIIEDERQRLARELHDSVS
                     QQLFAASMMLSAIKETKLEPPLDQQIPILEKMVQDSQLEMRALLLHLRPLGLKDKSLG
                     EGIKDLVIDLQKKVPMKVVHEIQDFKVPKGIEDHLFRITQEAISNTLRHSNGTKVTVE
                     LFNKDDYLLLRIQDNGKGFNVDEKLEQSYGLKNMRERALEIGATFHIVSLPDSGTRIE
                     VKAPLNKEDSYDD"
     gene            complement(24240..24941)
                     /locus_tag="O2701_001024"
     CDS             complement(24240..24941)
                     /locus_tag="O2701_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HCZ4292929.1"
                     /translation="MTHKYISTQMLIIFTALMIIANFYYIFFEKIGFLLVLLLGCVLV
                     YVGYLYFHKIRGLLAFWIGALLIAFTLLSNKYTIIILFVFLLLLIVRYLIHKFKPKKV
                     VATDEVMTSPSFIKQKWFGEQRTPVYVYKWEDVQIQHGIGDLHIDLTKAANIKENNTI
                     VVRHILGKVQVILPVNYNINLHVAAFYGSTYVNEKSYKVENNNIHIEEMMKPDNYTVN
                     IYVSTFIGDVEVIYR"
     gene            complement(24956..25342)
                     /locus_tag="O2701_001025"
     CDS             complement(24956..25342)
                     /locus_tag="O2701_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292930.1"
                     /translation="MNYVERYIEQFLRATVRNNIKHYLLMLDEKMKNLDDYMRYLITK
                     KEQLSKLIDSLMLTLENKYIDIAEAFQIQCAREINNQEIENIKSELNKVEAYYAQIET
                     QIQQTSTEKIATEKTSYLINYMNAVA"
     gene            complement(25559..26317)
                     /gene="map"
                     /locus_tag="O2701_001026"
     CDS             complement(25559..26317)
                     /gene="map"
                     /locus_tag="O2701_001026"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCZ4292931.1"
                     /translation="MIVKTEEELQALKEIGYICAKVRNTMQAATKPGITTKELDNIAK
                     ELFEEYGAISAPIHDENFPGQTCISVNEEVAHGIPSKRVIREGDLVNIDVSALKNGYY
                     ADTGISFVVGESDDPMKQKVCDVATMAFENAIAKVKPGTKLSNIGKAVHNTARQNDLK
                     VIKNLTGHGVGLSLHEAPAHVLNYFDPKDKTLLTEGMVLAIEPFISSNASFVTEGKNE
                     WAFETSDKSFVAQIEHTVIVTKDGPILTTKIEEE"
     gene            26845..27831
                     /locus_tag="O2701_001027"
     CDS             26845..27831
                     /locus_tag="O2701_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCZ4292932.1"
                     /translation="MNDQWYKHLIGARTIKTGIAIFLTAVFCMALDLTPIYAILTAVV
                     TIEPTAKASLIKGYRRLPATVIGAGFAVLFTYLFGDQSPFTYALSATFTILFCTKLKL
                     QVGTNVAVLTSLAMIPGIHDAYIFNFLSRTLTAIIGLVTSGLINFMVFPPKYYGQVEE
                     KLSKTDALMYKLFYNRCQELILSRLQSDKSEKAYKNIFNLNNQVETLISYQRDELSYH
                     KKKECDWKLLNQLTKRAYTNRLFITHLSNIIYLPKNTRVNFSGDEKMALLKISSSIKD
                     IFYDGSFKREDDSVETLRSTIKALEISGENQIKSHILYEVLMIYRLLDSRYA"
BASE COUNT         9096 a         4809 c         3975 g        10072 t
ORIGIN      
        1 aacaacttaa aacatttgtt ttttaaatat tagtgccgtt tgacgacttt tatataatat
       61 caagttattc tagtagagtc aacataaaac aacacttatt tttgtttaaa aattactgtt
      121 gaagggatta ttttctattc gtaagtacta tatgtattta aagtgtaatt ccttatgctt
      181 tttacttcag ttttacaaac aaacttatac tcactttatg gatagttatt tagtagacct
      241 tcgtttgaat atttttatgt ttatttataa aggatactag ttattagtta ataccacttc
      301 cgtagttaga atattctttt cttcaattta aatatttttc agaataagca taaaaaaaag
      361 ttaaacaact ttcatgtcgt ttaacttctt aaatattacg cttacttata tgaagtaagc
      421 taacgctttt ttaatttgtc atactaccaa agttaaatta tttatcttgg cattctttac
      481 aaactccata aatttccatt cgatgatgtg ttacgtcaaa gtcagtcata tgctgagcta
      541 atctttcaat ttcatttaac tgtggatatt gaaaatcaac aatcttacca cattgttcac
      601 atataatatg ataatgatta tgtgtattaa agtcgaatcg acttgatgag tctccatatg
      661 ttaattcttt tacaattcca atatctttaa acactcttaa gttattatat attgtcgcaa
      721 cacttatatt tggaaaatca ggtgaaagtg cttgataaat ttcatcagct gttggatgag
      781 tatgtgaaga aattaaataa cgtaatattg cttgtctttg aggtgtaatt cttacgcctg
      841 cttgtcgcaa tgatgcaatt gattcttcta gttcatgttc aattgattct atttcaacac
      901 tcatctatat caccatcttt cttatataat aattattatt acttactata aatataactg
      961 tagtattgcg cgcgtgtcaa tatttacatg atgatttcat ttaatagcct tttttagcat
     1021 caacttcatt ctcaattaga ccattcttat tgagaaaatt aactagattg tttttaaaaa
     1081 tatctaataa gtcatacttt gcttcataat cattaccagt tatatgcgct gttatagtta
     1141 cattatccaa ttcatataat tcatgattag gtttcaaagg ttcattttca aacacatcta
     1201 aatatgcatg tcgaataact ttacttttta atacttctat taagagcgct tctttaacta
     1261 tgctacctcg tcctatattt ataaaaagtg cttcatcttt cattaattca aaatgttttt
     1321 tctttagtaa atgaatcgtt tcttgcgttt ctggtaaagc atttataata atgtcagcat
     1381 ttggtaatgt gctttcaagt gattcgatag tatatatctc gtcaaactca tctttgttct
     1441 gacctgactt gctcagacca attaaattca tattaaaagc ctttgctaac ttcgcagttc
     1501 tagtagcaat tgcacctgta cctaaaaata aaactgtttg tcctgatagg cgtttaccag
     1561 ttattttcga atcatatata tgttgtcgtt ggttatcata tgatagtttc atctttttat
     1621 aatcatctaa aatgaaagct aaaatgtatt cagataattg tttagcttga acaccttttc
     1681 cattagttaa aagtatgccg tgatctgcaa tataatctaa cggcaatgta tttacacccg
     1741 ttgcaaacca tgcaatccat tttaaattcg ggcaacgtcg taaaaatgcc tcattgatac
     1801 cgccatcata accaactaat atatctaaat cagccaagtc gctctcaggt atttctgatg
     1861 cttttttata aaatttaaaa tctaaatctg aaaagcgttg ttgtaattca gtttcaactt
     1921 cacgcatacg atttaaccca acaactttca tatatgtcac cccaaaatgt ttttaagttc
     1981 ttctatttgt gttttaacct taaccttctc gataacatct aatactttac cttgttcatc
     2041 tattataaaa gtcgttctta caatgcccat actttctttg ccaaatgatt tttttaactg
     2101 atatacgcca gtttctttag ctaatttaaa atcttcatct actaataaat cgaaattcaa
     2161 tccgtgtttc tcaataaaat tttggtgttt tttctttgaa tcaccgctta taccatatac
     2221 tgcaacatct aaatcattga acatttctaa attgtctcta aagtcacaag cttctgtggt
     2281 acaagtaggt gtattatctc taggataaaa atatataatc gcctttttac cttttaatgt
     2341 atcatttgta atgacagttc cgtcttgatt ttctaattta aatattggaa attgttctcc
     2401 tttttgcaac atataatcac ctttttcttc attataataa ttttatgata cgatattaga
     2461 tgaaaatatt aaattaaaag aggttgatat aaatgaattt tagtgaaagt gaacgtttac
     2521 aacaactttc aaacgaatat attctaggcg gtgtcaattc cccttctcgt tcttataaag
     2581 ctgtaggagg cggtgcacct gttgttatga aagaaggaca cggtgcatat ttatatgatg
     2641 tcgatggcaa taaatttatt gattaccttc aagcatacgg tccaattatt acggggcatg
     2701 cacatcctca tattactaaa gcaattcaag aacaagctgc taaaggtgtt ttatttggta
     2761 caccgactga attagaaatt gaattcagca aaaaattacg tgatgcaatt ccatctcttg
     2821 agaaaattcg ctttgtaaat tctggaacag aagcagtcat gacaacaatt cgtgttgcac
     2881 gtgcatatac taaaagaaat aaaattataa aatttgctgg atcttatcat ggccattctg
     2941 atttagtatt ggttgcagca ggtagcggcc catctcagct cggttctcca gactcagctg
     3001 gtgttccaga aagcgtcgca cgtgaagtca ttactgtacc tttcaatgat attaacgcct
     3061 ataaagaagc aattgaattt tggggtgatg aaattgccgc agtattagta gaaccaattg
     3121 ttggtaactt tggaatggta atgcctcaac ctggattttt agaagaggtt aatgaaattt
     3181 cacataacaa tgggacccta gtgatttatg atgaagtaat tactgcattc cgtttccatt
     3241 acggtgccgc tcaagattta ttaggtgtta tccctgattt aactgcattt ggtaaaattg
     3301 ttggcggtgg tttaccaatt ggaggctatg gtggacgtca agatattatg gaacaagtag
     3361 cacctctagg acctgcatat caagctggta caatggctgg taacccgtta tctatgaaag
     3421 caggtattgc attactcgaa gtactagaac aagacggtgt ttatgaaaaa ttagacagct
     3481 taggccaaca actagaagaa ggtttactta aattaatcga aaaacataat atcacagcta
     3541 caattaatcg tatttatgga tctttaacat tgtactttac agatgaaaaa gtcacacatt
     3601 atgatcaagt tgaacattct gacggcgaag cgttcggtaa atttttcaaa ttaatgttaa
     3661 atcaaggtat caatttagca ccttctaagt ttgaagcttg gttcttaaca actgaacata
     3721 cagaagaaga tattaaacaa actttaaaag ctgcagacta tgcttttagt caaatgaaat
     3781 aacttttaag gaagtatatt tttaattgaa ttaaaataaa gcaaatgctc acatagtaga
     3841 ttcattttat atcgttaact attaacgttt aacttgatat gtcactatgt gagtttttat
     3901 tttacaaatt tatactattt taatctctaa ctaaagtttt acatcgctat cagtattatg
     3961 tatgatttat tttaacaact ataaataagc ttgaattttg taactagtaa gtgtataaca
     4021 gtaatgaata cgaatattag atattcaata aacttaaaag gggcgtcatt atttttgaga
     4081 ctaggagctc ggatttttaa aactggtata gccattattt tagctatgtc tatcgcttct
     4141 ttactaccgg atgatgttgg tctgaaagcc ttagctggtg tcagtgctgt tgttgcaatg
     4201 caacctagta tttatcgttc atttaaaaca gtttctgatc aagcattggg taacattata
     4261 ggtgccatac tatctgttac aatggtaaca atttttagtg ataattttat cattatgggt
     4321 gttaccgtca tagtacttat tgctatttta tttaaattta atttagcaca tgttgctact
     4381 ttggcgagtg taacagcatt aatcattatg ggacaacata ccggttcttt ctatattacc
     4441 gccttttaca ggtttgtact tgtaatgatt ggtgtcataa gttcatctct agttaacttt
     4501 gtgtttctac cacctaaatt tgaaacaaaa atttactata attcattaaa tatatcctca
     4561 gatattttta tgtggtttaa attagtatta aatgatacga cggagtttaa taatattaaa
     4621 caagatagtc ataatttaaa acaacgtgtc gaaaagttag agaaaattta tgattactat
     4681 agcgaagaac gtcctattac aaaaaaacat attcaccaac agaatagaaa gaaaatactt
     4741 ttcagagaag tagttcagac gactagacaa gcttatgaag tattaaacaa attgtcgcgt
     4801 tatcaaaacg acttatattt acttaataac aatttcctat tacaaatcaa attagattta
     4861 gattcattaa ctgcttttca cgagcaaatt ttagctagcc tttctaaaaa agcacgttat
     4921 aatgtgacac atgttgatta tgagttagat aatccgcaga aaaaagattt attgtccaca
     4981 tttcagcatg agttaataaa tcatccttat caaacggaat attcgtttgc caatgttatg
     5041 caaattgttg cagccatcga agagtatcga caccatttag agcatttaga ccgcataaga
     5101 attagctttt tcacatatca tagatctgat gcagacattg aaattgttga agaagacttt
     5161 gatttataac attaaaacaa ttttatcaat taatgccaca atgagataaa ctaatattga
     5221 atctcgaaat taaatttaaa aaagcagtaa gattattttc aattaagaaa atctcttact
     5281 gctttttcta tgttatgacc ccaccatcct gttacaaatt ttctcttcaa gattgtactg
     5341 aagtcaaaca actaatactt tataagtttt gaatgctata taaatgctcg taagcacctt
     5401 gttttgcaat caattcacga tgcgtacctg tttcaacaat atgtccattt tcaattacga
     5461 caattttgtc agcatgtgta atagtggaca agcgatgcgc tacgataagt gtcgttcgat
     5521 ctttactcaa cacatctaat gcttcttgaa taatggattc actttctaaa tcaagtgcac
     5581 ttgttgcttc atccaagata agaattggcg gattatttaa aaatattcta gcaatcgata
     5641 atctttgttt ttgaccacct gataatttaa cacctcgttc acctacttca gtgtcatatc
     5701 cctgtggcaa gttcataata aagtcatgtg cattagccat tttcgccgct tcaactactt
     5761 cttcatctgt tgctgttgga cgaccaagta aaatattttc cttaactgta tcagagaata
     5821 aaatattatc ctgttgcacc aatcctattt gatttcttaa acttcccgtt aaaaaatctt
     5881 taatgttgtg accatctatt aaaatttgcc cagaagttac atcgtaaaat ctcggtatta
     5941 agttaattaa tgttgattta ccaccaccac tcatacctac gaaagcaact gtttctcctt
     6001 tttcaatact caaattaata tcttttaaaa ttggagcttc gttatcgtta tattgaaaac
     6061 taacatgatc aatatcaata cgaccttgtt taatttcaat aggttgagca ccaacaccat
     6121 ttttgatgtc ataatcttca tcaattaatt ggaatacacg gtccattgaa gcaaaacttt
     6181 gcgttaaagt tgtaaatgat gcgactaaac gacgtaaagg cccgaacaat aactctaagt
     6241 atccaacaaa tgctgcaagt gtacctactg tgattgatcc agaaatagca agatatgcac
     6301 caacaccgat gacaataatt ggtccaatat ctgtaactgt attaattgcg gcaaaggaat
     6361 aggcattcca tcttgtatgt ttcaacgcac gtgttaggaa attagtattc tttttatcaa
     6421 agtttttcgc ttcattgtct tcaatcgcaa aacttttaac gactgaaata ccttgaacac
     6481 gttcatgcaa gaatccttga acctcagcta atgcttgaga tctttcacgt gtcaattttc
     6541 ttaatcttcc aaagaaaacg tacaccgtta aaatgtaaaa tgggaagata aacagtgctg
     6601 ctaaagtcaa tttcacatct aaaaagaaca ttatggatag tgcaataata attgttatac
     6661 aatctaacca aatattcatt aacccggtta aaatgaaatc ttttgtttgt tcaacatcat
     6721 taatcactct agatattact tgacctactt gattattagc ataaaatctc gcacttaaag
     6781 cttgtaaatg gttgtataac tttttacgta tatcatacaa tattttatta cttgtccatt
     6841 gcgccaaata ttgacgtata aattcaattg gtggtctcac tattacaaaa ataaataatg
     6901 cgataccaat ggcaatagtt aaatgatgaa ctttttcatc agtcgttagt gcgtggttat
     6961 taatcacgcc atctattgca tatttaatta atagtggtat aagcattggt ataccaaact
     7021 taattatccc aacaataatc gttgcaaaaa tacgatattt gtatggctta acaaattgca
     7081 aatatcgttt aatcatacaa tcccccctaa tctattgccc tatcctattc ataagcataa
     7141 aaatgaatag aggttggata cataatttgt agatgtaaat tcttcttaca atttacattt
     7201 ttaaaattaa gatatctcaa cctctgtcat attcatgtga ttaaatacgt tgtcttattc
     7261 gtatgttata ttctattcaa tttaatctat ggatactgtg tccccacacg acagcaaaag
     7321 ttatcatact tctttacatc actaagtcaa tataaatgat ttaatcagta tttacacttt
     7381 atttgcttaa tactgtctaa tttttttgta acgttctttc caaactttga taaaatctgg
     7441 cgcgaatggg cccttcttct gttctatcca ttgttgaaga atgtccacgt tgcgtcttaa
     7501 aataatatca atatcatgcg gataattcat ttgattcata tgttgctcat attcatcttc
     7561 atctaataaa tgatactttc cgtttggata tactttaata tctaaatcat agtctatata
     7621 ttttaatgcc tcttcatcac aaacaaatgg tgatgacaaa ttgcaatagt aataaattcc
     7681 atcttctcta aacatgcaga taacattaaa ccaatattct gagtgaaagt aaacaattgc
     7741 cggttcacgt gttatccaag ttcttccgtc actttcagtc actaacgtat gatcatttcc
     7801 accaatgaca acatgatcag taccctttaa tattgttgtt tcagaccaaa cgcgatgaat
     7861 cttaccatca tgtttataac tctgaatttt aatgttttcc ccttctttag gtatggattc
     7921 tctgaccata ctccacacca ccttctgtta atttaaccat tataaattat agcatatttc
     7981 agaaatagta ttatataaat acatattttt acgaaataag attttactac ttaataatta
     8041 aactcggtaa tattgctaag tactacaaca gagatttaca tgtcccattt aaagtatata
     8101 aaatcatcac ttttatatat caacacttta actttttgac attgttattc tatgagattt
     8161 aaagatatca tttatacttt ttaaaattaa tgtcactatg ttttccgata atattaccaa
     8221 tcatcgaatg ttacccattt ataaattgat aaatctttga cataggtaca gggaatgtat
     8281 attgatctcg atcacttaaa tcaaaccaaa tcatgtcatc tggtaatgtt tcaatgttaa
     8341 ttgctcctga aacggcgtat actttaatct tccatgttaa atgagtaaat tgatgcttca
     8401 actcaaaaat aggtgtttct actggttgaa tgtcatgacc gattttttca gtcattttac
     8461 gtctagcatg ctcactttca aacataggaa attgccacat accatgcaat aatttttcgc
     8521 tacgcttttg caacagatat tgaccttgat tatttctaat taaaaagacg gattgctcaa
     8581 ttactttttt acttacattt ttagatttaa caggtaactt ttcaaatgta cctttatcaa
     8641 atgcctcaca gttttcttga actggacaaa ataagcataa tggatttttt ggtgtacaaa
     8701 ttaacgcccc taattccatc atagcttgat taaacgttcc agcttctgta gtaacatacg
     8761 gtaataattc ttgttcgtac gatttcctcg tcgattgtaa tttaatatct cgatagtcat
     8821 cattcaatct agaccatact cgaaaaacat ttccgtctac agttgctagt ggtacattat
     8881 atgcaatgct cattactgca gcttgtgtgt atgggccaac accttttaac gctttaaatt
     8941 gatcaggatc tttgggaact aagccttcat atttatcatg aacttcttta atcgccgtat
     9001 gaaaatttcg agctctacta taatatccta agccttccca atactttaac acttcatctt
     9061 ccgaagcttg actcaaaact tccacagttg gaaatcgttc aacaaaacga tgataatagt
     9121 caataactgt tttaacttgt gtctgttgta acatgacctc acttaaccaa atatagtacg
     9181 gattggtcgt ttgtcgccat ggcatttctc tttgattttc atcaaaccag tgtatcaaat
     9241 tttctttaaa actagactgc tgatacattt ataaaaccct ttcctcacca aaattaattg
     9301 tctttactca taatgttttt attgtacatt aaaatcatgg ttagtatgta agttaattta
     9361 gttatttgcg aaattggatt ataatagtat atataatatt atgaaatgag tgaactgata
     9421 tggacactgc aacacatatc gcaattgggg tgggccttac agcacttgca actcaagatc
     9481 cagcaatggc ttctacgttt ggtgcaacag ctacaaccct tatcgttggt tcattaattc
     9541 ctgatgggga tactgttctt aaattaaagg acaatgcaac atatatttcg catcatagag
     9601 gtatcacgca ttccatccct ttcacaatac tatggccaat tttaattaca tttttaatat
     9661 tcacgttctt tagtggaacc aacccatttc atgtatggat gtgggctcag ctcgcagtat
     9721 ttttacatgt ctttgtagat atattcaatt cttatggtac acaagcgctt agacctatca
     9781 caaacaaatg gattcaatta agtgtgatta acacatttga ccctattatt ttcacagttc
     9841 tttgtattgg tattgtatta tgggttatag gcttgcatcc atttgcagtc ttctttccta
     9901 taatcgcttt actaatcatt tattacatga ttcgttttaa aatgagagcc gtaattaagc
     9961 aacaagcttt aaaagcaatt caacaagagc atcaccctgt taaagtattt gttgcgccaa
    10021 caataaaatt tatggaatgg cgtgtcgcga tacaaactga tgcacatgac tatgttggaa
    10081 aagcatatgg taggaatgtg gtgtttagtg ataaagtgga acgtcaaaca ttatcaacag
    10141 actccatttt atggaaagtc aaaggtaata aagatatacg tactttttta aacttttcat
    10201 caatctatcg ttggcaaaca acaacgttag cagatggttc tactgaaatt cgtttgattg
    10261 atttgcgtta tttaaaaaat gatcattatt catttgtggc aattgcacat gtaacaaacg
    10321 ataatgtcat agaccactct tatattggct gggtatttac agaagataag ttacaacgta
    10381 aactgtatgc taaataattt caagttatta ttcactaaag ttaatctata aaaaatgaac
    10441 aaccgggcag aatgaaaatc aaaacgattt ttactctgtc cggtttttta atgtaaaact
    10501 atgaatgctt ttacaaaatc taaaatttat attgttgcta acaaactacc tttaatgact
    10561 cgaaatatca aaatcagtat aggaaaacaa tatctagatg atattctaat tgtttctgat
    10621 tctcacagat taatttacac aacaggtcag ctaaacatca tgaagaagta tccgcctcgt
    10681 ctgtactatc atttgaaaca tcctgttgat tatcagtttg tgtcacttta gatgttttat
    10741 tataaattgc gtgtgtcgta taccttgcta atacgaaatt tactgcagct attaaacata
    10801 agaataaaat aaagtaaaaa tgatatggaa tgttttccat acttgatata ccaaatatga
    10861 tagattgtgc tataccatta acaatgtaat acattggatt tagcattagg atgtgattga
    10921 taaatacatg atttggattt ggtatgaaaa taattggtaa caataagaaa cacaatacac
    10981 aaaccccata aaatatgata tttatttttt cagttaacag tcgaataaga ccaaaagtaa
    11041 cggatattaa tcctacaaaa atagttgcca tcacaataaa atagaatagc gctatatatg
    11101 atgtttcgaa gtttactggt ttaaccaatg cactaatcat cgtcaaaatg actagcataa
    11161 taaaacttaa aatagacata ataactactg gcgtcgctgc ggaaatatta aataacttag
    11221 taactaaaaa gtcattcata taatgatgat aagagatata aattgtgaac atcactaccg
    11281 caaaaacaat caatacaatg attctaaata accatctcac ctctttaaca tcggtcaaat
    11341 gactaaagga aaatagcgtt tcacttgcaa taataagtat taaacatgca gctaatacga
    11401 ttagaaaact tttccaactt tgttttaaac gttctgttac atacctttta gtatgcggta
    11461 agtttttaaa atataaaata atattatcta gcatctttac acctacaatt caatataaat
    11521 ccatttgtct tctttcaaat tggcaataca atatccattc ccaactttag taatccaaat
    11581 gttattgtta atattaaact tatcttttaa ttctttttta tctacaattg gataaacaaa
    11641 accgtataac tgattattat cattgaaaag taattgtatc ggttgttgtg taacggatgc
    11701 aacgaaacgg ttatcatcat caggaactaa agtttttata actgaactat gctttttatg
    11761 taattgacta ttaaagtatg catagtaatt actataattt gatttcatat atggtgctaa
    11821 ttttttaaaa ctatgcgttt gatataaacc tgccgggtta aaatattcta atttatcttt
    11881 actaacatag taagtcttat tatcggcggt aacacgataa tttttactat tttcgccagt
    11941 aattgtaact acactatatt ttggaattgt gacttgttta tccccttgca tatccacact
    12001 gccattaaaa gcaattccat aagctaactt ttcgccatat ggatctttat tttcattttg
    12061 catcgtagca cgattcgtaa aatctgttat cgaaataatt cctatattat tgaaaatgag
    12121 caacatcatc aatgctaagc ctaaaatagt acctgaggcc aaagtccaaa aacgcactaa
    12181 aaacttaggc ggttttgcat gattatagcg ctcaacacgt ttgaaattat aggtcatctc
    12241 tggtattctt gtacgattct ttttccaatc taaatcaaag ttttcaattt cttcttttga
    12301 gtttagactt aatcgatcac gttcatgttc tttaaaagat ggaataactt gtttaagtga
    12361 cccttccatt cttaattgac catgtgaaaa ccaagctatg tagttactca cttgtgaaat
    12421 tttatcaata tcatcaccaa ttgacacaat cgttaaatta ttttcaatat aatcatttgt
    12481 taattcaatc gcacgttcca taaattgtgg tgttaaatag tcaataacat gatttaaaat
    12541 aataatattt gattttgatg agcgtgcaat acttaatagt aattgagcgt atgccgcttt
    12601 cgaaatatgg ttaaccggct tcgttttata atcacctaaa tgtgcatatt gaataatctg
    12661 ttcagcttta tgatcattaa tttcatatgg aaatagttga actaactgcg ctgtataagc
    12721 ttcaacagtt tgatgaatta acgattgatc ttcaatatat ccgtagaaca aatcttcagt
    12781 acaaactact ttacctttat caggtttaat tgcacctgcc aacaattgac ctaccaacgc
    12841 tttggaagat tcaggttcac caattatacc taatgcttct ccttgataaa tatgtaaact
    12901 aatattgttt aaatcgatat cttcagcatc atatccaaaa ggtaaatacc atttcttatt
    12961 ctgtttattc ctatagtagt gtgttacttt tagtaacttt aaaacaattg aacttcccat
    13021 ctattttcat ccttctataa ttgtaataac gctataggta atccttcttc gggttcatcg
    13081 ctattaaatc taaaccccca cgcaaaaata ccttttaatc tttctacttt aaaatattga
    13141 ttactgccat cagtcagttt ataaatttta ccaatttcta tcttttttct gtctactaag
    13201 taactttcgg caacaattac tttactttga taaacgtcat attcatttaa aataccattc
    13261 atctcagctt ttctcatttt ctctttataa agttgtattt cgtgtcttaa ttctggctca
    13321 gacatctcac tcaatttctt ctgttccatc ggcaatacca ctttcttcta atttagcttt
    13381 aattttatca tatttatatc cttttctcat aaggccttcg atagtttttg aaattaattt
    13441 ctgttgcgtg tacttctttc gatttttatt ataaattttt tctaaatctc gttgtaataa
    13501 atcgtctaaa accgcttcat cttgtgtaaa atccatttca ttcaatacag catgaatggt
    13561 ttccatttca aacccttttt gaattaaaga ttgcattact ttttctttaa ctttattttg
    13621 tggccctttt tttgttttag atattttttc agcaatttgg ataatatcat caagttcctg
    13681 ttgttctcta taaagttctg taaatatttc aatgatattt ggttctatac caagttgata
    13741 tagtttttgt tgataaattt taggtccttt atctgtcgtg cgaatcattg tattttttaa
    13801 actttccgca taatcttgat ggtcgattaa cttttcgcga taacaatatt caatcacttc
    13861 agaaatcgct tgctctgata tctcttcttt ttgtaaatat tgtataactt ctttttcagt
    13921 tctcttttta tatgataaat attggattgc tttatttaaa cctatgcgat aatgatcata
    13981 cttttgaatc tctgccatgt cagcagcttc aagttgttgc ccttttttta aattaaattt
    14041 gactaatgta tcaatatcta tacccatttc aaattgttcg tctaaaaaaa gattaaaccg
    14101 ttctttgttt ttcttttgaa cttctatttt agtaatcttc ggcactggat caccccgtta
    14161 ctttcttaag ttttaataca atactctttt attattcaat aagccacttc ctatagcaaa
    14221 tgtttaactt taaatatttt tcgatgctaa caaaaaatca cactatcatc ttttaaaatg
    14281 aaagtgtgat tacaagcaaa tctgtaaaat ttataaagca gaaacaattc aactttatca
    14341 ttatgacatt tcaattaaac cttctacatt atagttccaa gcatcttaca catgaatgca
    14401 agtatttaac gatttaattg tgacatagcc tgttgatatt gtgtttcatt gatataattt
    14461 tgttgcttca ttttttctaa gttcgtgctt acacgttgcg taaaattctc tgacatatta
    14521 ttgatattat atacgctagg tgcattgact ttactagcta aaatagcgct ttgtaaaact
    14581 gttatgtgag acattgttgt actattttta ttcacggttg ttccaaagta atggtttgct
    14641 gcgccctcaa gcgtatattg attatcccca aagtaaatat tatttaaata aaagcttaaa
    14701 atttcgttct tattatattg tttttcaact cgatgagcta caaataattc ttttactttt
    14761 ctagtaaatg aacgatcatt atcataaaaa taatttttga caacttgttg tgtaatggta
    14821 ctaccacctt gcacatctct gtcgctaatc gttgaaaata aagctctagt tgtacctttc
    14881 aaatcgaatc catgatgatt gtagaatcgt tcatcttcca ttgaaataaa ggcaccttta
    14941 acatactctg gcatgttatc agctgacaca aaactacttt tattttcaat ttttcttagt
    15001 tcatccacat tatcgcgtgt agataaaaaa tacatgatac caataaacaa tgcgataatg
    15061 attagaatgg ttaataatat ttttaatagt attcgtttac tttttttctt tttcggcggt
    15121 ttgccaactg gttgataata cgtattatag tgaggttcgt gtttcatatg ctcaaaatgt
    15181 tcatttgagt ttgagtacct atcgcttctt ttcatgcgtt tgctccttct tttaaaactc
    15241 acttagtata taccttgagt ttaccagtac tatcacaaat aggctacact ttttgggaaa
    15301 atcagtccaa gggcttacaa tcgtatacgc catcatactt acttttttgt tttttgaaaa
    15361 aattatagat aaatcattgc aattttaaat attaatcatg tcaaatattg ttatatttta
    15421 taaaaataaa agaccatccc tattaaatgc caatagagac gaccttttat ttgttattca
    15481 tttattaaaa ctaaaatcca tatttcattt caaacgaaaa tatataaatt ttaacaatcg
    15541 ataaccacaa tacttctatt gtaattgttt aacgatttct cgattaaaat catctaaatc
    15601 gtctggtact cgacttgtta caatattgtt gtctacaact actgactcat caactacatg
    15661 tgcgcctgca tttgataaat ctttgcgtac atttaatact gctgttaacg tacgaccttt
    15721 taaatcgtct gtatctatta gtatttgtgg cccatgacaa atggcaaatg ttggtacatc
    15781 atttttagta aagtatttag caaatgtgcc atatcgacct tctgtatctc cacgtaaatg
    15841 atctggtgaa aatcctccag gaattaataa tgcatcataa tcttctggtt tagcttctgc
    15901 aatgcctaca tcgacagtaa ctttttcacc gtgtttacca acaacttcac tatttgcagt
    15961 atctccaatc actacagtat taaagcctgc attctctaat gcctctttag ggcttgaata
    16021 ttctatatct tcaaattcgt ttgctagaat aattgctact tttttagtca ttgcaaatca
    16081 cctttctata tatcattgat ataattacta tagacaagta aatcagtgat taaacataca
    16141 agatataaaa aatattaagc gactgtcgcg atatctaacc ctaacacatc ttatgtggca
    16201 tttacttaga tactaattta accttttctt caagctgatc taacaatcca atccattcat
    16261 ctatatcttc aacacgtact tcatcaggat ttacatgatc gatatcctca ataaacttat
    16321 ttaaacgcgc ttttatctgt tcgattgttt gttgttcatt cataaaaagt taactccttt
    16381 tattttgttt tctttttcat tattatccta acagaaattg cgttaaagcg atataatctt
    16441 agctatattt atgacattca aattattttg acttttaaaa atcccctttt caattaacta
    16501 aaattaagag ataatttgtt acgagtgata atacgaagtg gtatcatacc gatatgaacc
    16561 aaatagaaag aaggaagttt aagacgatga atagcgtcaa attgaagcaa cctgttagca
    16621 tttacaatga tccatgggaa gcatataacg atgttaaaga acatggccaa ttaactttaa
    16681 gtaacatcga atttacaact acaaatcttt gtaatatgcg ttgtagccac tgtgcagttg
    16741 gttatacttt acaaactgtc gaccccgagc ctttagatat ggacttaatt tatcgtagac
    16801 ttgatgaaat tccaaatctg cgaacgatgt ctattacagg tggcgaacca atgttttcta
    16861 aaaagtctat tagaaatgtt gttaaacctc tattaaagta tgcacatcat cgaggtatat
    16921 atacacaaat gaattcaaac ctaacattgc ctcaagatcg ttatttagat attgctgaat
    16981 atatcgatgt tatgcatatc tcacacaact ggggaacaac tgatgaattc gcaaatgttg
    17041 gctttggcgc aatgaagaag caaccaccgt taaaagctaa gttaaaatta tatgaacaaa
    17101 tgatttcgaa tgcacgtaca ttatcagaac aaggaatgtt tgtatctgcg gaaacaatgc
    17161 tcaatcaaag tacgctacca catttacgaa aaatacatca agaagtcgtt catgatatga
    17221 aatgtagcag acacgagatt caccctatgt atccagctga ctttgcaagt caattaaatg
    17281 tgttaactct agcggaaatg aaaaagacaa ttcatgatat attggatttc agagatgaag
    17341 atatttggat gttatttggt actttgcctg tgtttccatg cttaaaggat gatgaagatc
    17401 aaaagttact atcacgttta agaaatgcta aaaatgtaac gactagaaat gacccggatg
    17461 gccgtagtcg tttaaatgtc aatgtattta caggtaatgt aatcgtaact gatttcggag
    17521 atgaaacagg tacaatttcg aatatacaaa aagataaatt aacagatgta tttgataaat
    17581 ggttatcctc tgatcttgct aaatcattaa attgtcattg ttccgagttt agttgtttag
    17641 gaccaaatgt tcttgttaaa aatatgtact atccgaatat ggattttaaa gataatgagc
    17701 gtcatatgca caaacaacca caaattatac aattttaaaa actcttaatt atgcggagaa
    17761 gcactttatc gataagtagt ctccgcatat tttaatgcta ttataaaata aaaaacaatt
    17821 aattgctggc agtactctac ttaaataata aagggcattt aataggacta atagtctata
    17881 ataaaagggg taaattttaa ctaaaagcat aaacgtgcat aatcaaaaag acagattgta
    17941 ggtggaatat tcgaacataa cagttcaatt catccttaac aatctgtctt tatattttta
    18001 gtctctaata tgttgcactt gagctaaata ttcaattgtt tgtttacttt caatgcgacg
    18061 tgcttttctt cgttcaacac gttgtggtgc tgtatcataa aaccattttt caacatcatc
    18121 ttctggatat acaccaggta catgtttagg tttgccttca tcatctaacg caacaaatgt
    18181 taagtaactt aatgcagcta aatcatgctt gttattaaat acatcgtcaa ttctaatttg
    18241 aacgaccact tccattgaac tagtcccagc gtatgaaacc atcgctacgt attgtaatat
    18301 gtcccctgtt ttaatcggct ttaagaaatc tactgagtct gtagatgcgg taactacttg
    18361 cgcaccagca tgtttcatag ctgtgattgc tgcaatttca tcaatattag ccatcaatgt
    18421 accaccaaac attgtatgat ggtgattcgt atcttgaggg aaaacttgtc tatttttata
    18481 acattttgat tctgacatag atttcattgg tctgtcctga tttgtcatta ttttatctcc
    18541 caatctatac tttaatcacg tatttgacct ttaaatacat gtttcatttc ctactcagca
    18601 cctcaaaata acatttatta aaatgttgat gcccaatttc cattttcaaa tactagttct
    18661 tttgaaccat cttcgaatat gccataaata gtcaaatcac tactaccaat catgaaatcg
    18721 acatgtacat ttgaatcatt taatccgctt gcaatctttt cctcaacagt catttccgtt
    18781 ccaccttgaa tattaaaggc gtaagcagat ccaatcgcta aatgacatgc tgcattttca
    18841 tcaaataaag tattgtaaaa aatggtatta cgatttgata tcggtgaatc atcaggtact
    18901 aatgctactt cacctaatct tcttgaacct tcatcagtat tgattaaatc tttcaatacc
    18961 gcttcacctt ttccagctga aaaatcaata atctctccat ctttaaacat caatttaaat
    19021 tgatcgataa ttgtgccatt ataacttaaa ggcaatttat tagtaacata tccatctact
    19081 ctatttcgat ctggtgctgt aaatacttct tcagttggta tattagcaat aaaagcttgt
    19141 tccttgccgt taacataact tgtagcatct tcccagatat gatttttcgc taaacctact
    19201 gttaaatcag taccttcaga tacataatgc aacgcatggt aatttttttg ttgtaacttt
    19261 tgagcgtaaa cacttaaatt agcgatgtgt tgacgccaat tttcaactgg atcgttacca
    19321 tcaattcgca caatatcgaa tacctcgtca ataaacttaa tatatgcttc ttctacagat
    19381 agttcaggat acactctctt cgcccaagct ttagatggaa aagcagctac gacccatgga
    19441 aattgatttt tttgtacact ttccatatat cctttaaatg cgcgtgcgtt ttgttgttga
    19501 aatgcttgta atttttggct atcgatacca tccattaaat caggatcttc actaataagt
    19561 gctaaattag cagcgccacg tttaacgtaa tccattctcg cttcaacatc atatgatttt
    19621 atctcatgat tagcaaaatg ttccacagat tcattttcaa attttaagcg ttttaaggtt
    19681 ggatcgctat atacaacacg tacatctgaa gcgccacaat gatatgcttc ttctacaatt
    19741 aaatgcgtta attctaatgt ttctactgat gatctaataa atactggctg ttttggttga
    19801 acattcatac caaccttaac taatagttca gcgtattgtt gtaacttttc tttgtaatta
    19861 gtcaaaattc ccactcctat tcgtgtcgca attgtcctaa agcgtcagca atagctgtca
    19921 cttcacttgg cgacaatctt tctcttgatt gaacaaaatc atatatatct gttaattctt
    19981 cttcatttgc atttttatat ttttcaggat ctaataatcc ttgattaaca atatttaact
    20041 tctctctaat agctaatact ttctcttcat ttgtcatagc cacaatgttc acctctaata
    20101 atagttatgt tatcaaagta tcacaattta ataaacttta atagaatttt atataatctg
    20161 ctttcttatt gtttaaagtc gctataaaat ggggatataa ctacatagtt tggaggaatc
    20221 aaaatggtag atgtagcatt tgtctgtctt ggcaatatat gtcgttctcc aatggcagaa
    20281 gcaatcatgc gacaaagact taaagacaga aatattcatg atattaaagt acattcaaga
    20341 ggtactggta gctggaattt aggagagcca cctcatgaag gtacacaaaa aattctcaac
    20401 aaacacaata ttccatttga tggcatgatt agtgaattat tcgaagcgac agatgatttt
    20461 gattacattg tggctatgga tcaaagtaac gttgataata ttaaatctat caatcctaat
    20521 cttaagggac aattgttcaa actgttagaa tttagtaata tggaagagag tgatgtacca
    20581 gatccatact acacgaataa ttttgaaggt gtatacgaca tggtattatc atcttgtgat
    20641 aatttaatag actacatcgt aaaagatgca aatttgaaag aggggtagtt ttttatggaa
    20701 aataaatttg ttcctggtat tttaattggt gccgtaattg gtggtgcaat tagtttagct
    20761 gataaatcta cacgtcaagc tttagttcaa tcagttaaag atgcaaaaaa tggtaaccgc
    20821 actcgtaagc cttctaaagt cagcaagatt aaagacgaag ttttatactg gaaagatgtt
    20881 gttgaagaaa ttcgtcgtaa taatcctgaa ttagaacgtt cattaaagga tgcgaaagaa
    20941 acatttgtta atagaaaaaa tcaacgctaa gatgattttg tttttataag gcaatactta
    21001 acaatttggt gtacgccttt catagttatc attattaaat gataatacaa tgattggcca
    21061 aactgcattg tttcagtatt gcctttgttt taattttaaa tcaaaatagc ctatgaaaga
    21121 tttaaatcaa ttaatttcta taatattatc atttttaaag catatcattg tttagttttt
    21181 ttataattgg ataaatacta atagttactt tataaaacat tacatagaga aaggttaagg
    21241 agtgcacatg tcgaaaaagg atcactcttc ttcaaaatac cttaattctg ttaaggaagc
    21301 gcaagaggag tcaaaaaaga aaaataaaag taatcccaaa attgatgttg atcgtacata
    21361 tattgaacct caacaattcc aatctaagaa acctaaaaaa gatgatcagg ttttcttctt
    21421 atcaagatta aataaacctg caaaatataa gaaagactct aatttcttat catatctcat
    21481 ctatcgcata ggaaaagatg atgcctcagg actagcagca caaatgactt accatttcgt
    21541 acttgctatg ttccctatgt tgcttttcct attaacatta ttaccatttt tcaatattaa
    21601 gcagagtcaa attactaata tgttaagcaa tgcacccgct gaaacatcta ctctaattaa
    21661 gagtgtaatt ggtgatataa ctcaaaactc cagtggtggc ttattatcta tcggtttgat
    21721 tttagcaatt tggtcagctt caaatggaat gactgcaatt atgaattctt tcaatgttgc
    21781 ttacgatgta gaagatagcc gtaatggaat cgtattaaaa ctactaagtg ttgtcttcac
    21841 tgtagttatg ggcgttgtgt ttgtagttgc tctagcatta ccaacgcttg gttctgtaat
    21901 tagtcatttc ctattcggtc cacttggatt tgacgaacaa gtgaaatgga tttttaacct
    21961 tattagaatt gtgttaccaa tcattattat atttatcata tttatcgtgt tatattcggt
    22021 tgcacctaac gttaaaacga agcttaagtc agtattacca ggtgcagtat ttacttcaat
    22081 tatttggtta gctggttcat ttggttttgg ttggtatatt tcaaattttg gtaactattc
    22141 taaaacatat ggcagtatcg cgggtatcat cattttgtta ctatggttat atatcacaag
    22201 ttttattata attgtcggtg ctgaaatcaa tgcaatcatt catcagcgta gtgtaattaa
    22261 aggtaaaaca cctgaagaag cagcattaga acatgatgac aataataaaa atcattataa
    22321 tgagaacact agatacgaat atgatgaaga tagtaatgca acacatcaac gtacgtatca
    22381 tgttgatgaa catcctagtg atacctatcc agaagatgat aaaaatataa aagacaaaat
    22441 cgttgataag cttaaaaaag actaaacacc aacaaaacag aggttttcgc taatgattat
    22501 tgcgaaaacc tctgtttttt ggtgtctatt tgatgttata ttgtgattgg cgtaagtaac
    22561 ttttcttaat tcgatatgaa ctattgaatt aaattatgtt ggaatgcata gataacagct
    22621 tgtgttctat cttgcacttc taacttactt aaaatgttac tcacatgtgt cttaaccgtt
    22681 ttaatagtaa tatgcgatgc actagcaatt tcttgatttg agtaaccttt cgcaatcaat
    22741 aataatattt ccatttctcg ttctgtaagc atttcatata actctgcgcg ctttttcata
    22801 cggttacgca ttttcactaa aacttccggt tcaaaaacag attctcctct agaagtttta
    22861 cgaactgcat cggcgatatc ttttgcactt gttgttttta aaatgtaact atcgacacct
    22921 gcatctaatg cacgatatac ctctttatct tcaataaaac tagttaacat taatacttta
    22981 atttgcggta aatctttttt aatctgagtc gtcgcttcta caccatccat gtcatccata
    23041 agtaaatcca ttaaaattaa atctggcttc aactcatggg ctttggcaat tgcttcttta
    23101 ccagaagcgc cttcaccaac tacttcaata tcactttgcg ttgatagata acttgaaatt
    23161 cctatacgta ccatttcatg atcatccaca aacaatactt taatcgtcat acgaatcctc
    23221 cttatttaaa ggtgctttca cctcgatacg tgtacctgaa tctggcaatg atacaatatg
    23281 gaacgttgca ccaatttcca aagctctttc acgcatattt ttaagtccat aactttgttc
    23341 taatttttca tcaacattaa aacctttacc attatcttga attctcaaca ataaataatc
    23401 gtctttatta aacaattcta ctgtcacttt tgtaccgttt gaatgacgca atgtattcga
    23461 aattgcttcc tgtgtaattc tgaacaaatg atcttcaata cctttaggca ctttaaaatc
    23521 ttgtatttca tgcacaactt tcattggcac ttttttttgt aaatcaataa ctaaatcttt
    23581 aataccctca cctaaagatt tgtcttttaa accaagcggt cttaaatgta acagcaaagc
    23641 acgcatttct aactgcgaat cttgaaccat tttctctaaa ataggaattt gttggtctaa
    23701 tggtggttct aacttcgttt ctttgatagc agatagcatc atacttgccg caaaaagttg
    23761 ctgactaaca gaatcgtgaa gttctcgtgc tagtctttga cgttcatctt caataatctt
    23821 tttaactttc acatcattaa tattataatt ttcattggtt aagttttgag ttttaagtcg
    23881 caacttatgc aattcttgat ttaaaggtac gagtgtatgg tataaatcta acgtttcact
    23941 atatatttct atattttgat cattaatgcc aactgtttcg ccttccattg aacgctcaat
    24001 ttgcgtctta atccaatcat tttgctgatt gattttgtaa gcgagtaccg aaccaacaat
    24061 aatacacaat aatatgatga tgagatttaa aaataaaaaa actggtattc cgaatatttg
    24121 tgtataaaac ataccttgaa aatagatgat atttacaaaa actttatcga tgaacagaaa
    24181 tgcagctagc atgctatata ctaagatgag cattgaacca attgttctaa tgtagtggtt
    24241 catcgataaa tcacctctac gtctccgata aacgttgata cgtagatatt aactgtatag
    24301 ttatccggtt tcatcatttc ttcaatatga atattgttat tttcaacttt atatgatttt
    24361 tcattcacgt aagtacttcc ataaaaagca gctacatgta aattaatatt gtaattaacc
    24421 ggcaatataa cctgcacttt acctaaaatg tgtctaacaa caatggtatt attttcctta
    24481 atatttgcag cttttgttaa gtcaatatgt aggtcgccaa ttccatgttg aatttgtaca
    24541 tcttcccact tatatacata aactggtgta cgttgctcac caaaccactt ttgtttaata
    24601 aaagatggtg aagtcataac ctcatccgtc gcaactactt tttttggttt aaacttgtgt
    24661 attaaataac gcacaataag taataataaa aagacgaaca agatgatgat tgtatactta
    24721 ttagacaata atgtgaatgc aattaatagc gcgcctatcc aaaacgccaa aaggccacgt
    24781 attttatgaa aataaagata tcctacataa actaatacac atcccaataa tagaacgagt
    24841 aaaaagccaa ttttttcaaa aaatatgtag taaaaattgg caataatcat taatgcagta
    24901 aaaatgatca acatttgcgt tgatatatat ttgtgtgtca tgtttcgccg cctttctatg
    24961 ccacagcgtt catataattt attagatacg atgttttttc tgttgctatt ttttcagttg
    25021 aagtttgttg aatttgagtt tcaatttgtg cataatatgc ttcaacttta ttcaactctg
    25081 atttaatatt ttcaatttct tgattattga tttctcttgc acattgaatt tgaaatgctt
    25141 cagcaatatc aatatattta ttttctaatg ttagcattag actgtcaatt aacttgctaa
    25201 gttgttcttt tttagtaatt aaataacgca tataatcatc taaatttttc attttttcat
    25261 ctagcattaa aaggtagtgc ttgatattat ttcttactgt tgctctcaaa aactgttcaa
    25321 tataacgttc aacatagttc ataactatca ccttttataa taagttttaa aataccaaat
    25381 gcgctatgta tcttcgttga tgtcgatgat atgtttgctt aaaaactact ttgttacaac
    25441 atatttttga aatgacgcat tgattgtgtt cttaattatt ataaaacagt tccgaaacca
    25501 atatgaatag gctccggaac taaatgatgt tcatacttta gtcttagtat atgttgaact
    25561 attcttcttc aatctttgtc gttaaaatcg gaccatcctt agtcacgata accgtatgct
    25621 caatttgagc aacaaaactt ttatcgctcg tttcaaaagc ccattcattt ttaccttctg
    25681 taacaaatga tgcatttgat gagataaacg gttcaatagc taataccata ccttcagtta
    25741 ataatgtttt gtcttttgga tcaaagtaat taagtacatg tgctggtgct tcatgtaatg
    25801 ataaaccaac accatgacct gttaagtttt taatgacttt caaatcattt tgtctagctg
    25861 tattatgcac cgctttacca atgttactta acttagtacc cggttttact tttgcaattg
    25921 cattctcaaa tgccatcgtt gctacgtcac atactttttg tttcattgga tcatctgatt
    25981 ctccaacgac aaatgaaatg cctgtatctg cataatagcc attcttcaaa gccgatacat
    26041 caatatttac taaatctcct tcacgaatga cacgcttact tggaatccca tgtgccacct
    26101 cttcattgac actaatacac gtttgaccag gaaaattttc atcatgaatt ggcgcagaaa
    26161 tagcaccgta ttcttcaaat aactctttcg caatattatc aagctctttc gtagtgatac
    26221 ctggtttggt tgcagcttgc attgtattgc gcactttagc gcatatgtat ccaatttctt
    26281 ttaacgcttg taattcttct tctgttttta caatcatttt atcccgttcc ttttttatat
    26341 aattataact tattatagca aaaattcttt gatatactaa agatgttact ttagtatttt
    26401 ggaaagtgag tgagacatag tgtgataaag aaaaaatatt tctttatcgt tgtaccccaa
    26461 cttgcattgc ctgtagaaat tgtagatcca atttctctat gttggggccc cgccaacctg
    26521 cacattatcg taagctgact tttcgtcagc ttctgtgttg gggccccgcc aacctgcatt
    26581 gcctgtagaa tttctttttt gaaattctct atgttggggc cccggactat aattgaaaaa
    26641 tgcttgttac aagtgcattt tatttcagtc aactactaac aatataacat tgtggagccc
    26701 agaactttga ttaatgtata tgaaaatcaa agtaatgctt atgtatgatt atttcaaata
    26761 ttttacatac atgaactttt ccaatgtacg atactattat tataaagcgc tcgctaagat
    26821 tttacgatga ttagaggtta aaaaatgaac gatcaatggt ataaacattt aattggtgcc
    26881 agaacaatta aaactggtat tgccattttt ttaacagctg tcttttgtat ggcactagat
    26941 ttaacaccca tctatgccat tttaacagct gtagtcacaa ttgaaccaac tgccaaggca
    27001 tcacttatta aaggttatcg tagattacct gctacggtaa ttggtgcagg ctttgcagta
    27061 ttatttacat atttattcgg cgatcaatca ccttttacat atgcattgag tgcaacgttc
    27121 acgattttat tctgtacaaa actcaaattg caagttggta cgaatgtcgc tgtactaaca
    27181 tcattagcta tgattccagg tattcatgat gcctacatat tcaacttttt gtctcgaaca
    27241 ttgacagcaa ttattggact cgtgacatca ggtttaatta acttcatggt gtttccacct
    27301 aagtattatg gtcaggtcga agaaaaatta agtaagacag atgccttgat gtataaatta
    27361 ttttacaatc gctgccaaga acttatctta tcaagactgc aatctgataa aagcgaaaaa
    27421 gcatataaaa atattttcaa tttaaataat caagttgaaa cgttaataag ttatcaacgt
    27481 gatgaattaa gctatcataa gaaaaaagaa tgtgattgga aattactcaa tcaattaact
    27541 aaacgtgcat atacgaatcg tttgtttatc actcatttat caaatatcat ttacttgcct
    27601 aaaaatactc gtgttaactt ttcaggtgac gaaaaaatgg cattgctaaa aattagtagt
    27661 agcatcaaag atatatttta tgatggttct tttaagagag aagatgattc tgtagaaact
    27721 ttacgttcga ctataaaagc acttgaaatt agtggtgaaa atcaaattaa aagtcatata
    27781 ctatacgaag ttctaatgat ttatcgactg ctagacagca gatatgctta atttaagtac
    27841 cttatgctaa ttggcaccat gggagtggga cagaaatgat attttcataa aatttatttc
    27901 gtcgtcccac cccaactcgc tttgcctgta gaatttcttt tcgaaattct ct
//
LOCUS       DALAGD010000030        27264 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.030, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000030 DALAGD010000000
VERSION     DALAGD010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 27264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27264
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.030"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(114..1118)
                     /locus_tag="O2701_001028"
     CDS             complement(114..1118)
                     /locus_tag="O2701_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HCZ4292933.1"
                     /translation="MSIIIEKSGLFSSFQDFGRRGYEHDGVIPCGALDTLAHEIANRL
                     VANDKNEATLEMTNKMATIRFTEPTLIALAGGNVKAYTEHMTISPYKLYLLDKGDVLK
                     FRETSYTSRVYLAVGGGFELDAWLGSNSTDFNVKIGGFKGRTLQDGDEIKLKRDYTAR
                     HHKLFENLAHTKQTDWGIDGYALSFNYMSDVFHVVKNKGTEDFKEDAIQRFVKHDYKV
                     TSKANRMGMMLEGEKIKAFYEDMPPYQTVKKGTIQIKRDGTPIILLNDHYTLGSYPQI
                     GTIASYHLTKLAQKPQGSRLKFQFIDILTAEKNLVKYSNWLNQLFHGIEYRMQLEMMK
                     "
     gene            complement(1102..1812)
                     /locus_tag="O2701_001029"
     CDS             complement(1102..1812)
                     /locus_tag="O2701_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase subunit 1"
                     /protein_id="HCZ4292934.1"
                     /translation="MKIYSQGDQAIVVAIEKEVSKSLTEDLLTLRSYLIEQNYPFIIE
                     IVPSESDMMIVYDARDMIKHHNIQSPFLYMKALIESIHLNIKHDFNQQDLIEIPIVYG
                     SKYGPDLESLLKHYKIKLETFIELHSKAQYFVSMMGYSPGFPYLTGLNKKLYINHTSK
                     QKKFIPAGSVVLEGKKCGIVTTDTINDWLVIGYTPLSLFNPKESDFARLKLGDNIKFR
                     PINENELEVGAFKDVNHN"
     gene            complement(1900..2106)
                     /locus_tag="O2701_001030"
     CDS             complement(1900..2106)
                     /locus_tag="O2701_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292935.1"
                     /translation="MIIVYIVLLLILVYVNYRLVNRLLSENRIYVVRLIATITTVISF
                     ILVYALIHELMPFVVRAMDLMYHQ"
     gene            complement(2172..2780)
                     /locus_tag="O2701_001031"
     CDS             complement(2172..2780)
                     /locus_tag="O2701_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HCZ4292936.1"
                     /translation="MQLFETMKIDNGHIPRLTYHTNRIKCSSERLNFKFDEHAWRNEL
                     NDVTTKYHSGQYRLKIVLNAESKFETIVSPLPEKSSFTAKFQVLPKVVNPTFIKNKTT
                     ERKHLAHNHETDLILLTSEDGKVLEFDIGNIVIEEDGKWYTPSYKDDFLKGCMRDYLI
                     DSDKLVEKDFNKNELIYKYHNNEIRLFLINSLREVADVHLCL"
     gene            complement(2780..3931)
                     /locus_tag="O2701_001032"
     CDS             complement(2780..3931)
                     /locus_tag="O2701_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HCZ4292937.1"
                     /translation="MRIEYNYRYYLTENEYKQYHIQLKGFIKKYVATKLADVGEVIHF
                     AQAQQRQGRYVSLYLSYEAAKYFNHAMCTHSLAKDDIYAAAYSFEKAESINSTYEHQT
                     SYVSKYHFSFVESSEVMMTNIKRVQQAIVEGETYQVNYTARLTDNIYYPISTLYERLT
                     QFSNGNYTALLQTDEIQVASISPELFFQKGQFNNVDNVIISKPMKGTMPRGKTEAEDQ
                     QYYKTLQTSSKDRAENVMIVDLLRNDIGRISQSGSIKVYKLFFIEAYKTVFQMTSMVS
                     GTLKNNTDLTQILTSLFPCGSITGAPKLNTMKYIKQLESSPRGIYCGAIGLLLPTEDD
                     KMIFNIPIRTIEYKYGQAIYGVGAGITIDSKPKDEVNEFYAKTKILEML"
     gene            complement(3915..4508)
                     /locus_tag="O2701_001033"
     CDS             complement(3915..4508)
                     /locus_tag="O2701_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HCZ4292938.1"
                     /translation="MILVIDNNDSFTYNLIDYIKTQTKLTVQVVGIDNLLIEDVINMK
                     PKAIVISPGPGNPDDYPILNEVLEQFYQRVPILGVCLGFQCIVSYFGGNIIHGYHPVH
                     GHTTQLRHTNEGIFQGLPQNFNVMRYHSLIADGATFPNCLKITAKNDEAIIMAFEHIR
                     FPVFGVQYHPESILSEYGYRQVELFLSKVGDYCENRI"
     gene            4859..5527
                     /gene="queC"
                     /locus_tag="O2701_001034"
     CDS             4859..5527
                     /gene="queC"
                     /locus_tag="O2701_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCZ4292939.1"
                     /translation="MESVLNNEKAIVVFSGGQDSTTCLFYAKKHFKEVELVTFNYGQR
                     HDTEIEVAKQIAQDQGMKHHVLDMSLLSQLTPNALTQHDMEITNNEDGIPNTFVPARN
                     LLFLSFAGALAYQIGAKHIITGVCETDFSGYPDCRDSFIKSMNVTLSLAMDKDFVIHT
                     PLMWLNKAETWKLSDELEVLDYIRTKTLTCYNGIIGDGCGECPACHLRQRGLNQYLES
                     KGAL"
     gene            5529..5948
                     /gene="queD"
                     /locus_tag="O2701_001035"
     CDS             5529..5948
                     /gene="queD"
                     /locus_tag="O2701_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCZ4292940.1"
                     /translation="MLQQIYPSTTHPYQFELNKDFNFSAAHHIPCEEAGICQNVHGHT
                     YFVNLTIVGDKLDDTGFLVNFSHLKKMIHGKFDHQLLNNLPAFKNKIPSTEIVAETIY
                     QIVKENLASLEHQPKCIQVFVRETPTSYVVFRPKEQV"
     gene            5952..6665
                     /gene="queE"
                     /locus_tag="O2701_001036"
     CDS             5952..6665
                     /gene="queE"
                     /locus_tag="O2701_001036"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCZ4292941.1"
                     /translation="MAKIPVLEIFGPTIQGEGRVIGRKTMFVRTAGCDYRCSWCDSAF
                     TWDGSAKGDIKLMTAEEIYDELKRIGGDLFNHVTISGGNPALIKGIQELVDLFQDKGI
                     FSALETQGSKFQPWMTQIDDLTISPKPPSSTMTPDLKKLDEVITQCVPSSLNLKVVVF
                     DDKDYDFAKMIHHRYPDIPFYLQVGNPYLSDSVDNHTEKLLERYEQLVDLVMQSNDMN
                     HVYVLPQLHTLLWSNKKGV"
     gene            6764..7348
                     /locus_tag="O2701_001037"
     CDS             6764..7348
                     /locus_tag="O2701_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292942.1"
                     /translation="MIVYILINIAIVVLITGFNLYRHQMQHLSLSAMLLSITINAFIN
                     TFIIDKYNFITLCTITMFIIWTILQFYIDKKLKPVYITDQKFIAIILTIVVSLTQRVT
                     DFSSTQSIYMSIPFLAPAIFIIGGIMLFISTFNSLDETAENNNKIKKLMIKGLIIINI
                     SFIVMMVLTPYWYLYLIVYLIFLLFLLWQKVYKF"
     gene            7628..8068
                     /locus_tag="O2701_001038"
     CDS             7628..8068
                     /locus_tag="O2701_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HCZ4292943.1"
                     /translation="MNTKYFLAAGAVITTLALGACGNSNSQDQGNKTEQKTKSEDSNV
                     KTDKTKHLTGTFSSKNGETVEGKAEIKNGKLMLTNYKSSKGPDLYVYLTKNGDIKNGK
                     EIAMVDYDKEKQTFDLKNVDLSKYDEVTIYCKKAHVIFGGAKLK"
     gene            8410..8883
                     /locus_tag="O2701_001039"
     CDS             8410..8883
                     /locus_tag="O2701_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCZ4292944.1"
                     /translation="MNKLLLLVTFIIRVGSGIVMLMQGYEKLTGGFTLKGLVPVIANN
                     TDSPEWYKWFFANIVAHTTSLFDIVVPLGEIAIGLGLIFGVFAYAASFFGAFVMINYI
                     LADMIFTYPLQLTFFILLLMSHSLLKQISLKEIINYFRGRKNRGEKIDDPLTDRG"
     gene            8858..9544
                     /locus_tag="O2701_001040"
     CDS             8858..9544
                     /locus_tag="O2701_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCZ4292945.1"
                     /translation="MTHLLIVDDEQDIVDICQTYFEYEGYKVTTTTSGKEAISLLSND
                     IDIMVLDIMMPEVNGYDIVKEMKRQKLDIPFIYLTAKTQEHDTIYALTLGADDYVKKP
                     FSPRELVLRINNLLTRMKKYHHQPVEQLSFDELTLINLSKVVTVNGHEVPMRIKEFEL
                     LWYLASRENEVISKSELLEKVWGYDYYEDANTVNVHIHRIREKLEKESFTTYTITTVW
                     GLGYKFERSR"
     gene            9544..10599
                     /gene="saeS"
                     /locus_tag="O2701_001041"
     CDS             9544..10599
                     /gene="saeS"
                     /locus_tag="O2701_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SaeS"
                     /protein_id="HCZ4292946.1"
                     /translation="MVLSIRSQIIIGVVSSILLTSTILAIAYILMWFNGHMTLTLTLT
                     TIITSCLTLLICSIFINPLIQKIKQFNIKTKQFANGNYASNDKTFNSPKEIYELNQSF
                     NKMASEITQQMNQIKSEQQEKTELIQNLAHDLKTPLASIISYSEGLRDGIITKDHEIK
                     ESYDILIKQANRLSTLFDDMTHIITLNTGKTYPPELIQLDQLLVSILQPYEQRIKHEN
                     RTLEVNFCNEIDAFYQYRTPLERILTNLLDNALKFSNVGSRIDINISENEDQDTIDIA
                     ISDEGIGIIPELQERIFERTFRVENSRNTKTGGSGLGLYIANELAQQNNAKISVSSDI
                     DVGTTMTVTLHKLDITS"
     gene            complement(10671..11654)
                     /gene="csbB"
                     /locus_tag="O2701_001042"
     CDS             complement(10671..11654)
                     /gene="csbB"
                     /locus_tag="O2701_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosylation protein
                     CsbB"
                     /protein_id="HCZ4292947.1"
                     /translation="MKIRVVIPCFNEGEVITQTHQQLTEILSQDSSVKGYDYNMLFID
                     DGSTDTTIDEMQHLATIDRHVSFISFSRNFGKEAAMIAGYQHSTEFDAVIMIDCDLQH
                     PPEYIPKMVEGFMEGYDQVIAKRDRSGENFSRKTLSHLYYKLVNCFVEEVQFDDGVGD
                     FRLLSQRAVKSIASLEEYNRFSKGLFEWIGYNTKVFTYQNVERQKGESKWSFKKLFNY
                     GIDGLISFNSKPLRMMIYLGLFIFSISVLYIIYLFINIMISGVNIPGYFSTIAAILLL
                     GGIQLISIGVVGEYIGRIYYEVKARPKYIIQATNLSSLENDEKDTHKVYSK"
     gene            complement(11786..12625)
                     /locus_tag="O2701_001043"
     CDS             complement(11786..12625)
                     /locus_tag="O2701_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCZ4292948.1"
                     /translation="MLNEIQILNNGYPMPSVGLGVYKISDEDMTKVVNAAIDAGYRAF
                     DTAYFYDNEASLGRALKDNGVDREDLFITTKLWNDYQGYEKTFEYFNKSIENLQTDYL
                     DLFLIHWPCEADGLFLETYKAMEELYEQGKVKAIGVCNFNVHHLEKLMAQSSIKPMVN
                     QIEVHPYFNQQELQEFCDRHDIKVTAWMPLMRNRGLLDDPVIVKIAEKYHKTPAQVVL
                     RWHLAHNRIIIPKSQTPKRIQENIDILDFNLELTEVAEIDALNRNARQGKNPDDVKIG
                     DLK"
     gene            complement(12847..14196)
                     /locus_tag="O2701_001044"
     CDS             complement(12847..14196)
                     /locus_tag="O2701_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCZ4292949.1"
                     /translation="METSTIISLIIFILLIALTTVFVGSEFALVKIRATRIEQLADEG
                     NKPAKIVKKMIANLDYYLSACQLGITVTSLGLGWLGEPTFEKLLHPIFEAINLPTALT
                     TTISFAVSFIIVTYLHVVLGELAPKSIAIQHTEKLALVYARPLFYFGNIMKPLIWLMN
                     GSARVIIRMFGVNPDAQTDAMSEEEIKIIINNSYNGGEINQTELAYMQNIFSFDERHA
                     KDIMVPRTQMITLNEPFNVDELLETIKEHQFTRYPITDDGDKDHIKGFINVKEFLTEY
                     ASGKTIKIANYIHELPMISETTRISDALIRMQREHVHMSLIIDEYGGTAGILTMEDIL
                     EEIVGEIRDEFDDDEVNDIVKIDNKTFQVNGRVLLDDLTEEFGIEFDDSEDIDTIGGW
                     LQSRNTNLQKDDYVDTTYDRWVVSEIDNHQIIWVILNYEFNEARPTIGQSDEDEKSE"
     gene            complement(14417..15598)
                     /gene="nagA"
                     /locus_tag="O2701_001045"
     CDS             complement(14417..15598)
                     /gene="nagA"
                     /locus_tag="O2701_001045"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCZ4292950.1"
                     /translation="MSELIIYNGKVYTEDGKIDNGYIHVKDGQIVAIGEVDDKAAIDN
                     DTTNKIQVIDAKGHHVLPGFIDIHIHGGYGQDAMDGSYDGLKYLSENLLSEGTTSYLA
                     TTMTQSTDKIDNALTNIAKYEAEQDVHNAAEIVGIHLEGPFISENKVGAQHPQYVVRP
                     FIDKIKHFQETANGLIKIMTFAPEVEGAKEALETYKDDIIFSIGHTVATYEEAVEAVE
                     RGAKHVTHLYNAATPFQHREPGVFGAAWLNDALHTEMIVDGTHSHPASVAIAYRMKGN
                     ERFYLITDAMRAKGMPEGEYDLGGQKVTVQSQQARLANGALAGSILKMNHGLRNLISF
                     TGDTLDHLWRVTSLNQAIALGIDDRKGSIKVNKDADLVILDDDMNVKSTIKQGKVHTF
                     S"
     gene            complement(15906..17864)
                     /locus_tag="O2701_001046"
     CDS             complement(15906..17864)
                     /locus_tag="O2701_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCZ4292951.1"
                     /translation="MRVTELLTKDTIAMDLMANDKNGVIDELVNQLDKAGKLSDVASF
                     KEAIHNRESQSTTGIGEGIAIPHAKVAAVKSPAIAFGKSKAGVDYQSLDMQPAHLFFM
                     IAAPEGGAQTHLDALAKLSGILMDENVREKLLHASSPEEVLAIIDEADDEVTKEEEAE
                     AEAQQVATAEQSSKQSNEPYVLAVTACPTGIAHTYMARDALKKQADKMGIKIKVETNG
                     SSGIKNHLTEQDIENATGIIVAADVHVETDRFDGKNVVEVPVADGIKRPEELINKALD
                     TSRKPFVARDGQRKGNSNDSQEKLSPGKAFYKHLMNGVSNMLPLVISGGILMAIVFLF
                     GANSFNPKSSEYNAFAEQLWNIGSKSAFALIIPILSGFIARSIADKPGFASGLVGGML
                     AISGGSGFIGGIIAGFLAGYLTQGVKAMTRKLPQALEGLKPTLIYPLLTVTATGLLMI
                     YAFNPPASWLNHLLLDGLNNLSGSNIVLLGLVIGAMMAIDMGGPFNKAAYVFATGALI
                     EGNAAPITAAMIGGMIPPLAIATAMLIFRRKFTKEQRGSIIPNYVMGMSFITEGAIPF
                     AAADPLRVIPSMMIGSGIGGAIALGLGSRITAPHGGIIVIVGTDGAHLLQTLIALLVG
                     TLVSALIYGLIKPKLTETEIEASKSMDE"
     gene            complement(17870..18790)
                     /gene="pfkB"
                     /locus_tag="O2701_001047"
     CDS             complement(17870..18790)
                     /gene="pfkB"
                     /locus_tag="O2701_001047"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCZ4292952.1"
                     /translation="MIYTVTFNPSIDYVIFTNDFKIDGLNRATATYKFAGGKGINVSR
                     VLKTLDVESTALGFAGGFPGKFIIDTLNNSAIQSNFIEVDEDTRINVKLKTGQETEIN
                     APGPHITSTQFEQLLQQIKNTTSEDIVIVAGSVPSSIPSDAYAQIAQITAQTGAKLVV
                     DAEKELAESVLPYHPLFIKPNKDELEVMFNTTVNSDTDVIKYGRLLVDKGAQSVIVSL
                     GGDGAIYIDKEISIKAVNPQGKVVNTVGSGDSTVAGMVAGIASGLTIEKAFQQAVACG
                     TATAFDEDLATRDAIEKIKSQVTISVLDGE"
     gene            complement(18787..19551)
                     /locus_tag="O2701_001048"
     CDS             complement(18787..19551)
                     /locus_tag="O2701_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCZ4292953.1"
                     /translation="MMIITEKRHELILEELSHKDFLTLQELIDRTGCSASTIRRDLSK
                     LQQLGKLQRVHGGAMLKENRMVEANLTEKLATNLDEKKMIAKIAANQINDNECLFIDA
                     GSSTLELIKYIQAKDIIVVTNGLTHVEALLKKGIKTIMLGGQVKENTLATIGSSAMEI
                     LRRYCFDKAFIGMNGLDIELGLTTPDEQEALVKQTAMSLANQSFVLIDHSKFNKVYFA
                     RVPLLESTTIITSEKALNQESLKEYQQKYHFIGGTL"
     gene            complement(19801..20283)
                     /gene="ybaK"
                     /locus_tag="O2701_001049"
     CDS             complement(19801..20283)
                     /gene="ybaK"
                     /locus_tag="O2701_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HCZ4292954.1"
                     /translation="MAKNKKTNAMRMLDRAKIKYEVHSFEVPEEHLSGQEVAELIQAN
                     VKTVFKTLVLENTKHEHFVFVIPVSETLDMKKAAALVGEKKLQLMPLDNLKNVTGYIR
                     GGCSPVGMKTLFPTVVDKSCENYSHISVSGGLRTMQITIAVEDLITITKGKIGAVIHE
                     "
     gene            complement(20457..20909)
                     /locus_tag="O2701_001050"
     CDS             complement(20457..20909)
                     /locus_tag="O2701_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292955.1"
                     /translation="MTVKNLFLGFVAVILTVCLIGLLILATNEDALAKVHKTINTLNA
                     INVSTEDTYKKKMDILNIHTAKASEVNENVKKQNHFKHRVNANKSNSFNEQECQVIAD
                     RYADKHINDNYGLERISKTNHGYNYVYSNDNSTSKQHVSISNQGIITK"
     gene            complement(21202..22368)
                     /gene="norA"
                     /locus_tag="O2701_001051"
     CDS             complement(21202..22368)
                     /gene="norA"
                     /locus_tag="O2701_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorA"
                     /protein_id="HCZ4292956.1"
                     /translation="MNKQIFVLYFNIFLIFLGIGLVIPVLPVYLKDLGLTGSDLGLLV
                     AAFALSQMIISPFGGTLADKLGKKLIICIGLILFSVSEFMFAVGHNFSVLMLSRVIGG
                     MSAGMVMPGVTGLIADISPSHQKAKNFGYMSAIINSGFILGPGIGGFMAEVSHRMPFY
                     FAGALGILAFIMSIVLIHDPKKSTTSGFQKLEPQLLTKINWKVFITPVILTLVLSFGL
                     SAFETLYSLYTADKVNYSPKDISIAITGGGIFGALFQIYFFDKFMKYFSELTFIAWSL
                     LYSVVVLILLVFANDYWSIMLISFVVFIGFDMIRPAITNYFSNIAGERQGFAGGLNST
                     FTSMGNFIGPLIAGALFDVHIEAPIYMAIGVSLAGVVIVLIEKQHRAKLKEQNM"
     gene            22577..22999
                     /locus_tag="O2701_001052"
     CDS             22577..22999
                     /locus_tag="O2701_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCZ4292957.1"
                     /translation="MKLQKSNHTILLVLEKGEDIVECITTFADDQDLTFTSVSGIGAC
                     DDVVLKFFNLTTKQYEEKHITEPLELTSLLGNISRLDNGHFAHLHATFGTQSYETFSG
                     HLAKAIVSATAEIILTVTDLDIQRSFKDAVGLNLLDPQ"
     gene            23185..23802
                     /locus_tag="O2701_001053"
     CDS             23185..23802
                     /locus_tag="O2701_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HCZ4292958.1"
                     /translation="MQSRYIARIYFLILFIVSLFETKIFQFMTLNLFLAYIPFELSLL
                     LRLFKPNKKYEWPLFIIYSFIFVLLLPNTFYMVTDLIHLNQFQFNFYAGLNLSEWKYF
                     TYLLLGVFLAVYVMILIYFEILTLTSHRWLNRLIVVVLMFLNGFGIYIGRFLRLHSVY
                     FFNEPLHVLNHILESLTLKTAMFVCFMVVMQAAILLFGKGVRLNK"
     gene            23799..24083
                     /locus_tag="O2701_001054"
     CDS             23799..24083
                     /locus_tag="O2701_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292959.1"
                     /translation="MTINIIVLIVSIIVFQLIIGHIWHDIGLSYLRSILLMMLPFGLG
                     VFIQQVSYYERQYPKWQVPQNIKVRLKYIYLATFLEYVVLYLTLFTDILR"
     gene            complement(24238..25611)
                     /locus_tag="O2701_001055"
     CDS             complement(24238..25611)
                     /locus_tag="O2701_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCZ4292960.1"
                     /translation="MAIAVLLNRMFRMEHNPLFEYIYQQKEDIDACYFIIPEEDMSSA
                     SDLKAQFYRGTLQRFYQSLHAEKLTPYVMSYDDIISFCKENNISEVVTAGDIMSYHLE
                     EYDILHQRSLFNEARIAVTLIRGNHYFKASKTMNQQGEPYNVFTSFYKKWRPYLRHRD
                     VYHYDLKSFEDFVIASPDDLVFDDIAFGSSQIIEQNKWQHFLDQDIQNYESGRDYLPE
                     VLTSQLSVALAYGLLDIIEIFNDLLARYDEDEANYEAFIRELIFREFYYVLMTQYPET
                     SYQAFKPKYRQIKWSQNEADFNAWCEGQTGFPIIDAAIMELTQTGFMHNRMRMVVSQF
                     LTKDLFIDWTWGEKFFRKHLIDYDAASNIHGWQWSASTGTDAVPYFRMFNPIRQSERF
                     DAKALYIKTYLPIFNQIDAKYLHDTQRNESNLFEQGIELGSHYPRQMVDHQEKRTQVL
                     ATFKALD"
     gene            complement(25697..27250)
                     /locus_tag="O2701_001056"
     CDS             complement(25697..27250)
                     /locus_tag="O2701_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HCZ4292961.1"
                     /translation="MSEEKHVVEHEQQKKEKTKKQYKPFWIVMSFIILIVVLLLPAPS
                     SLPIMAKAVLAILAFAVIMWVTEAVSYPVSATLIIGLMILLLGFSPVQNLGEKLGNPK
                     SGSAILAGSDLLGTNHALSLAFSGFATSAVALVAAALFLAAAMQETNLHKRLALLVLS
                     IVGNKTRNIVIGAIIVSIVLAFFVPSATARAGAVVPILLGMIAAFKVSKDSKLASLLI
                     ITSVQAVSIWNIGIKTAAAQNIVAINFINHQLGFDVSWGEWFLYAAPWSIVMSVALYF
                     IMIKVMPPEINTIEGGKDLIKEELHKLGPVSPREWRLIVISMLLLLFWSTEKVLHPID
                     SASITIIALGVMLMPKIGVMTWKHVENKIPWGTIIVFGVGISLGNVLLKTGAAQWLSD
                     QTFGVLGLKHLPIIATIALITLFNILIHLGFASATSLSSALIPVFISLTSTLHLGDQS
                     IGFVLIQQFVISFGFLLPVSAPQNMLAYGTGTFTVKDFLKAGIPLTIVGYILVIVFSM
                     TYWKWLGLL"
BASE COUNT         9013 a         4939 c         3872 g         9440 t
ORIGIN      
        1 tgtgaaacga attagaaaat gatacatttc gcagataaaa cacagtatta tttcattata
       61 cattaggagg aaattatttg catcggactc gagtatgagc tacgtaccaa atattatttc
      121 atcatttcta attgcattct atattctatt ccatggaata attggtttaa ccagttacta
      181 tacttaacaa ggttcttttc agccgttaaa atatctataa attgaaattt caaacgtgat
      241 ccttgcggtt tttgtgctaa tttcgttaaa tgataacttg cgattgtacc gatttgcggg
      301 tagctaccta gcgtataatg atcatttaat aggataatag gtgtgccatc acgcttaatt
      361 tgtatcgttc cttttttgac agtctgatac ggtggcatat cttcataaaa agctttgatt
      421 ttttcacctt caagcatcat ccccatgcga tttgctttgc tcgttacttt ataatcatgt
      481 ttcacaaatc tttgaatggc atcttcttta aaatcttccg tacctttatt tttaacgaca
      541 tgaaatacat cagacatata attaaatgac aaggcgtatc catcaatacc ccaatctgtt
      601 tgtttcgtgt gagcaaggtt ttcaaataac ttatgatgac gagctgtata atctctctta
      661 agctttattt catcgccatc ttgtaatgtt ctacctttaa aaccaccaat ttttacatta
      721 aagtcggttg agttagatcc taaccatgca tctaattcaa atccgcctcc cacagctaaa
      781 tacactcgcg atgtataact tgtttctcta aactttaaaa catcgccttt atctaacaaa
      841 tacaatttat atggagatat agtcatatgc tcagtgtaag ctttgacatt accccctgct
      901 aatgcaatca gcgtaggttc tgtaaaacga atcgttgcca ttttattagt catttccaaa
      961 gttgcttcat tcttgtcatt tgcaactaat cgattagcaa tttcatgtgc taaagtatca
     1021 agtgcaccac atggaattac accatcatgt tcatatcccc ttctgccaaa gtcctgaaag
     1081 ctactgaata agccactttt ttcaattatg attgacatct ttaaacgctc ctacttctaa
     1141 ttcattttca ttgataggtc taaatttaat attatcgcct aactttaagc gtgcgaaatc
     1201 tgattctttc ggattaaaaa gtgataatgg tgtataacca ataactaacc aatcattaat
     1261 tgtatccgta gttacaatac cgcatttttt cccttcaagt actacagaac cagctggaat
     1321 gaattttttc tgtttactcg tgtgattaat atacaatttc ttatttaatc cagttaaata
     1381 aggaaaccca ggtgaatatc ccatcatcga aacaaaatat tgcgccttag aatgtaattc
     1441 aataaaagtt tctagcttga ttttgtaatg ttttaaaagt gattctaaat ccggaccata
     1501 tttcgaacca tacacaattg gtatttcaat caaatcttgc tggttaaaat catgttttat
     1561 gtttaaatga atcgattcta ttagtgcttt catgtataaa aaaggtgatt gtatattatg
     1621 gtgtttaatc atatcccttg cgtcatagac aatcatcatg tctgattctg atggcacaat
     1681 ttctataata aatggataat tttgttcaat taaatatgag cgaagtgtta ataaatcttc
     1741 ggttaaactt ttagatactt ctttttcaat tgcgactaca atggcttggt caccttgact
     1801 atatattttc atttgtacac ctcttcaata tttggttact ttactttcat ttttaattat
     1861 agaatcgatt gaatagttca ttttacctac tcactaaatt tactggtggt acattaaatc
     1921 cattgcccgc acaacaaaag gcataagttc gtgaattaat gcgtatacaa ggataaagct
     1981 tataacagta gtaattgttg ctatcaaacg aacaacatat attctatttt cagatagcaa
     2041 tcgattcact aatcgataat ttacgtatac aagaattaac aacagcacaa tataaacaat
     2101 aatcatattt ggccccattt aatttttaaa tttgttttca catcattttc tactttatta
     2161 tcatactaat tttaaaggca aaggtggaca tcggcaacct ctcgtaaact atttatcaaa
     2221 aataaacgta tctcattgtt atgatattta taaatcaatt cgtttttatt aaagtctttt
     2281 tcaacaagtt tgtcactatc tattaaataa tcacgcatgc atccttttaa gaaatcatct
     2341 ttataacttg gtgtgtacca ttttccatcc tcttcaatga caatgttgcc aatatcaaat
     2401 tcaaggacct tgccgtcctc tgaagttagc aatattaaat ctgtttcatg attgtgtgct
     2461 aaatgctttc gttctgtcgt tttatttttt ataaaagttg gattaactac tttgggcaac
     2521 acttgaaatt ttgctgtaaa actacttttc tcaggtaaag gtgacactat cgtttcaaat
     2581 ttgctttcag catttaatac gattttaagt ctatattgac cactgtgata ctttgttgtt
     2641 acatcgttta attcatttcg ccatgcatgt tcatcaaatt taaagtttaa tcgctcagaa
     2701 gaacatttta tgcgattagt atgataagta agtctaggga tatgtccatt atcaattttc
     2761 attgtttcaa ataattgcat tataacatct ccaaaatctt ggtttttgcg taaaattcat
     2821 tcacttcatc ttttggctta gaatcaattg taatacctgc tccgactcca taaatcgctt
     2881 gtccatattt atactcaata gtgcgaatcg gaatattaaa aatcatttta tcatcttcag
     2941 ttggaagtaa tagtccaatt gctccgcagt atataccacg aggtgaactt tctaattgtt
     3001 taatatattt cattgtattc agtttcggtg cacctgtaat cgaaccacaa ggaaataacg
     3061 atgttaaaat ttgagttaag tctgtattat tttttaaagt tccacttacc atcgaagtca
     3121 tttgaaatac agttttatat gcctcaataa aaaatagttt atacacctta attgagccac
     3181 tctgtgatat tctccctata tcgtttctta gtaaatcaac aatcatgaca ttttctgcac
     3241 gatctttcga agaagtttgc aatgttttat aatactgttg atcttcagct tccgttttac
     3301 ctctaggcat tgtccctttc atcggtttgc ttatgataac gttatcgaca ttgttaaatt
     3361 gtcctttttg aaaaaataat tctggtgaga tagacgctac ttggatttca tcagtttgta
     3421 ataacgcagt ataattacca ttactaaatt gagttaatcg ttcatataaa gtactaatag
     3481 gataataaat gttatccgtt aagcgcgccg tatagttcac ttgatacgtt tcgccttcaa
     3541 caattgcttg ttggacacgt ttaatattag tcatcataac ctcagaagat tcaacaaatg
     3601 aaaaatgata ctttgataca taagaagttt gatgttcata tgttgaattt atgctttccg
     3661 ctttttcaaa actataagct gctgcataaa tatcatcttt agctaatgaa tgtgtacaca
     3721 tagcatgatt aaaatacttt gccgcttcgt aacttaaata taacgagaca tatctacctt
     3781 gtcgttgctg cgcttgtgca aagtgtatca cttctcccac atcagccaac ttagtagcaa
     3841 catacttctt tataaatccc tttaattgaa tatggtattg cttatattca ttttcagtta
     3901 aatagtagcg ataattatat tctattctca cagtaatcac ctaccttcga taaaaataat
     3961 tcaacttgtc gataaccgta ttcactcaaa atagattcag gatgatattg cacaccaaaa
     4021 accggaaatc taatatgctc aaatgccata ataatcgctt catcgttttt tgctgtaatc
     4081 tttaagcaat ttggaaaagt cgctccgtca gcaattaatg aatgataacg cattacattg
     4141 aaattttgag gcagtccttg aaaaatacct tcattggtat ggcgtaactg tgtagtatgt
     4201 ccgtgtacag gatgatagcc gtgaatgata tttccaccaa aataagacac gatacattga
     4261 aatcctaaac atacacctag tataggtaca cgctgataaa attgttctaa cacttcattc
     4321 aagataggat aatcatccgg attacccggc ccaggcgaaa taacaattgc ttttggcttc
     4381 atattaatga cgtcttctat cagcagatta tcaataccaa caacttgaac tgttagtttc
     4441 gtttgagtct taatatagtc tattaaatta tatgtaaatg aatcattatt atctatgact
     4501 agaatcattg tatactccgt tctaaatgtg ttttattttt ataatatgta ttggatgtag
     4561 ctaaaacttt aaaagcattg tcattatcct gaccttgatt ttaactaata tatggtatat
     4621 tctattcatc gtacataaat gaatatcaga ggttcctagc tgaaaccctc tataaaaaac
     4681 tagacattga aatttcaaac attcgttggt aaacctttaa tatatattat aggagcaacc
     4741 catttctttc gtctatcttt tatggagata ggcttttttt attttataaa ttaaaaacgg
     4801 gcttatcaat gccattaacc tctttattaa tattattaat gataggagca attacaaaat
     4861 ggaaagtgta ttaaataatg aaaaagccat cgttgtcttt agtggtggtc aagacagtac
     4921 aacatgtctc ttttatgcaa aaaaacattt caaagaagtt gaactcgtaa cgtttaatta
     4981 tggccaaaga catgatactg aaattgaagt cgcaaaacaa attgcacaag atcaaggaat
     5041 gaaacatcac gttttagata tgtcattatt atcacaactt actccaaacg cattaacaca
     5101 acatgatatg gaaattacta ataatgaaga tggtatacct aatacatttg ttccagctag
     5161 aaatttactt ttcttgtcgt ttgcaggcgc tctagcttat caaattgggg ctaagcatat
     5221 tattacaggc gtatgtgaaa cagacttttc aggctaccca gactgtcgcg atagttttat
     5281 aaaatcaatg aacgtaacat taagcctagc tatggacaaa gattttgtca ttcatactcc
     5341 tttaatgtgg ttaaacaaag cagaaacgtg gaaattaagt gatgaactcg aagttttaga
     5401 ttatattcgt acaaaaacat taacatgcta taacggtatc attggggatg gctgtggtga
     5461 atgtccagct tgtcatttac gtcaacgtgg actaaatcaa tatcttgaaa gtaaaggagc
     5521 gctttaaaat gttacaacaa atctatccta gtacaacgca tccatatcaa ttcgaattaa
     5581 ataaagattt taatttttcg gctgcacatc acattccatg tgaagaagca ggtatttgtc
     5641 aaaatgtcca tggtcatact tactttgtta atttaacaat tgtcggtgat aaactagatg
     5701 acactggctt cttagtgaac tttagccatt tgaaaaagat gatacacggt aaatttgacc
     5761 atcaactgtt aaataactta cctgctttta aaaacaaaat cccttcaact gaaatcgtag
     5821 cggaaacaat ttatcaaatt gttaaagaaa atttggcatc gctcgaacac caaccaaaat
     5881 gtattcaagt atttgtaaga gaaacaccaa caagttatgt cgtatttaga ccaaaggaac
     5941 aggtgtaact aatggctaaa attcctgtac tcgaaatatt cggtccaacc attcagggtg
     6001 aaggtcgagt aatcggtaga aaaacgatgt ttgtgagaac agctggatgt gattatcgct
     6061 gtagctggtg tgattctgca tttacttggg atggtagtgc taaaggcgat ataaaactca
     6121 tgaccgctga agaaatttat gatgaattaa aacgaattgg tggcgattta tttaaccacg
     6181 ttacaatttc tggtggtaac ccagcattaa ttaaaggtat ccaagagtta gttgacttat
     6241 ttcaagataa aggtattttc agcgcactgg agacacaagg cagtaaattc caaccttgga
     6301 tgacacaaat tgatgattta acgattagtc caaaaccacc aagttcaact atgacacctg
     6361 atttaaaaaa gttagatgaa gtgattacac aatgcgtacc ttcatcatta aacttaaaag
     6421 tagttgtatt cgacgataaa gattatgatt ttgccaaaat gatacaccac cgttatccag
     6481 atattccctt ctatttacaa gttggtaacc catatttatc agacagcgta gataatcata
     6541 ccgaaaagtt gttagaacgc tacgagcagt tggtggattt agttatgcaa agtaatgata
     6601 tgaatcacgt ttatgtgtta ccacaacttc atacattact ttggagcaat aaaaaaggtg
     6661 tataaaggat aacttatttt actttttgca agtcatatct aatgaataac aatatattaa
     6721 ttgatataat agttgattga agtataacga acaggagtcc atcatgattg tatatatatt
     6781 aattaatatt gctatcgtcg tacttataac tggattcaat ttatataggc atcaaatgca
     6841 acatttatca ttaagtgcaa tgttgttatc tattacgata aacgccttta taaatacatt
     6901 cataattgat aaatataact ttattactct gtgtactata acgatgttta tcatttggac
     6961 gatactacag ttttacattg ataaaaaatt aaaacctgta tacattacag accaaaaatt
     7021 tattgctatc atattaacga ttgttgtcag tttaacgcaa cgtgttaccg acttttcaag
     7081 tacacaatca atatatatgt ctattccatt tttagcccct gccattttca tcattggtgg
     7141 tattatgttg tttatttcaa catttaatag cttagatgaa actgctgaaa ataataacaa
     7201 aataaagaaa ctaatgatta aaggacttat cattattaac atttcattta tcgttatgat
     7261 ggttttaaca ccatattggt acttgtattt aatcgtctat cttattttct tgttgttttt
     7321 attgtggcaa aaggtttata aattttaata ccaaaactat taaacacttc tgatattctt
     7381 agttcaaaat atcagaagtg ttttatagtg ttatctagtt cagataaata tttccttact
     7441 taaaaaaacg ccctcctctt attttgaccc ctatttattt aaatcagaca attattttca
     7501 ttttcaaatt attctttctt caatattagt taagcgatat ttaaacgaag ttaagaatta
     7561 gttaatggca tattatttgc cttcatttta aacttaactt atcaaattga agaaatgagg
     7621 agttagcatg aatacaaaat attttttagc agctggtgct gttatcacaa cattagcttt
     7681 aggtgcttgt ggtaattcta attcacaaga tcaaggtaac aaaactgaac aaaaaacaaa
     7741 gtcagaagat agcaatgtta aaactgataa aacaaaacac ctaacaggta cattcagttc
     7801 taaaaacggt gaaactgttg aaggtaaagc tgagattaaa aatggtaaat taatgcttac
     7861 taactacaaa tcatcaaaag gtccagattt atacgtctac ctaacaaaaa atggcgacat
     7921 taaaaacggt aaagaaatcg caatggttga ctacgataaa gaaaaacaaa catttgatct
     7981 taaaaatgta gatttaagca aatatgatga agtaacaatc tattgtaaaa aagctcacgt
     8041 catcttcggc ggcgctaaat taaaataatc aatttctgag ttaaactttt atttacaaca
     8101 tactattact atacattaca aatttttaaa atatacatta cactcattac tcaatggaaa
     8161 gcgtatgatt tcacagcccc cctagcttgt agaaatcata ctttcctttt ttcaatatat
     8221 atacaactat taaatcccat aagattgcag agcacataag taaatttttt tagagcttga
     8281 ggtttgttta gcttaagcaa cccatgagct caaacacttc ctgttcacat aacactacaa
     8341 atcgcattat gttgcttaat cttatgttta tataaattac acacaataaa tagaaagaat
     8401 gtgaacatca tgaataaatt attgctactc gttacattta tcattcgtgt gggttcaggt
     8461 attgttatgt taatgcaagg ctacgaaaaa ttaacgggcg gatttacgct gaaaggttta
     8521 gtaccagtca tcgctaacaa tactgattca ccagagtggt ataagtggtt tttcgcaaat
     8581 atagttgcac atacgacgtc attatttgat attgttgtcc cactcggaga gattgcaatt
     8641 ggattaggtt taatttttgg agtttttgca tatgctgcta gtttctttgg agcctttgtt
     8701 atgataaatt atatcttagc agatatgata tttacgtatc ctcttcaatt aactttcttt
     8761 atccttttac taatgagtca ctcattgtta aaacagattt cacttaaaga aatcattaat
     8821 tactttagag gtcgtaagaa cagaggtgaa aaaatagatg acccacttac tgatcgtgga
     8881 tgatgaacaa gacattgtag acatttgtca aacctatttt gaatatgaag gttacaaagt
     8941 aacaacgaca actagcggta aagaagcaat ttctttacta tcaaatgata ttgatatcat
     9001 ggtacttgat atcatgatgc cagaagttaa tggttacgac attgtcaaag aaatgaaaag
     9061 gcaaaaatta gatatcccct ttatctattt aactgccaaa acacaagaac atgataccat
     9121 ttacgcctta actttaggtg cagatgacta tgtcaaaaaa ccatttagtc caagggaact
     9181 cgttttacgt attaataatt tacttacaag aatgaagaaa taccatcatc aaccagttga
     9241 acaactgtcg tttgatgaat taacacttat taacttaagt aaagttgtga ctgtaaatgg
     9301 tcacgaagtc cctatgcgta ttaaggaatt tgagttattg tggtatttag cttctagaga
     9361 aaatgaagtt atttctaaat cagaattact tgaaaaagtt tggggatatg actattacga
     9421 agatgctaat accgtgaatg tccatataca ccgtattaga gaaaaattag aaaaagagag
     9481 ctttacaaca tataccatca caactgtatg gggattagga tataaatttg aaaggagccg
     9541 ataatggtgt tatcaattag aagtcaaatc attattggcg tcgtatcgag tatactatta
     9601 acttcaacta ttttagcaat tgcatatatt ttaatgtggt ttaacggcca tatgacacta
     9661 actttgacct taacgacaat aattacaagc tgtttaacct tattaatatg tagtattttt
     9721 attaatccac ttatacaaaa aattaagcag tttaatataa aaactaagca atttgctaac
     9781 ggaaattacg caagcaatga taaaacgttt aattcaccaa aagaaattta tgaattaaat
     9841 caatctttta ataaaatggc ttctgaaatt acgcaacaaa tgaatcaaat taaatccgaa
     9901 caacaagaaa aaacagaact gattcaaaac ttagcccatg atttaaaaac acctttagca
     9961 agcattattt catattctga aggactacgt gatggtataa tcactaagga tcatgagatt
    10021 aaagagtcat acgacatatt aattaaacaa gcaaacagat tatcaacatt atttgatgat
    10081 atgactcata ttatcacttt aaatacaggt aaaacatatc ccccagaatt aatacaacta
    10141 gaccaattac ttgtatcaat attgcaacca tatgagcaac gtatcaaaca tgaaaaccgc
    10201 acattagaag tgaatttctg taacgaaatt gatgcatttt atcaatatcg aacgccactt
    10261 gagcgtattt taacaaactt acttgataat gcgctaaaat tttcaaatgt tggtagtcgc
    10321 attgatatta atattagtga aaacgaagat caagatacta tcgacattgc tattagcgat
    10381 gaaggtattg gcattatacc agaactacaa gaacgtatat tcgaacgtac attcagagta
    10441 gaaaactctc gtaatacaaa aacgggtggt tctggattag gcttatatat agctaatgaa
    10501 ctcgcgcaac aaaataacgc aaaaatcagt gtaagcagtg atatagatgt aggaactacg
    10561 atgactgtaa cattacacaa attagacatt acgtcataat ccgatttatt tataaaataa
    10621 aatgcaaaga ctaaaaagaa gctcccatta atgagggctt ctttttttgt ttatttagaa
    10681 taaactttat gggtatcctt ctcatcattt tcaagacttg aaagatttgt agcttgaata
    10741 atatatttag gacgtgcctt aacttcataa tatatcctgc caatatattc acctacaaca
    10801 ccaattgaaa ttaactgtat gccgcctaat aataaaatag ctgcaatcgt tgaaaaatat
    10861 cccggaatat taacaccaga tatcataata ttgatgaata aatagataat atataacacg
    10921 cttattgaaa agataaacaa gccaagataa atcatcattc tcaaaggttt actattaaag
    10981 gaaatcaatc catcaatacc ataattaaat aactttttaa aggaccactt agattcccct
    11041 ttttgtctct caacattttg atacgtaaac actttagtat tatagcctat ccattcaaat
    11101 aacccttttg aaaatcgatt atattcttca agtgatgcaa tggatttaac agctctttgg
    11161 cttaaaagtc taaaatcacc aacaccatca tcaaattgta cttcttctac aaagcaatta
    11221 actaacttat aatacaaatg gcttaatgtt ttgcgactaa aattttcacc acttctatca
    11281 cgctttgcaa tcacttgatc atagccttcc ataaaacctt caaccatttt cggaatatat
    11341 tcaggtggat gttgcaaatc acaatctatc atgatgactg catcaaattc agtactatgc
    11401 tggtaacctg caatcatagc tgcttctttt ccaaaatttc tactaaaaga aataaagctg
    11461 acatgcctat ctattgtggc aagatgttgc atttcatcta tagtggtatc cgtactacca
    11521 tcatctatga aaagcatatt ataatcatag cctttcacac tactatcttg tgaaagtatt
    11581 tcagttaatt gttgatgtgt ttgtgtaatg acttcccctt cattaaaaca aggaatgacg
    11641 actctaattt tcatctatgt ccatcctttt tatttaaaag attttgaatg tacctgagta
    11701 ttgttttatc aaatagtaaa aaccaactca tttattttaa tgtacacatt aaaaggcatt
    11761 cataaacgta aaatttaaca tccagttatt ttaaatcccc aattttcaca tcatctggat
    11821 ttttaccttg tcttgcattt ctatttaaag catcaatttc agctacttct gttaattcta
    11881 aattaaaatc taaaatatct atattttctt gaatgcgttt aggtgtctga gattttggaa
    11941 taataattct attgtgtgct aaatgccaac gtaatacaac ttgtgctggt gttttatgat
    12001 atttttcagc aattttaaca atgacagggt cgtctagtag tcctctattt ctcatcaaag
    12061 gcatccatgc agtcacttta atatcgtgac gatcacaaaa ttcttgtaat tcttgttggt
    12121 taaaatatgg atgtacctca atttgattca ccattggttt gatacttgat tgagccatta
    12181 atttttctag atgatgaaca ttaaaattac atacacctat tgcttttacc ttaccttgct
    12241 cgtaaagttc ttccatagct ttatatgttt ctaaaaatag accatctgct tcacaaggcc
    12301 aatgtattag aaataaatca agataatcag tttgtaaatt ttcaatcgat ttgttgaaat
    12361 attcgaatgt tttctcataa ccttgatagt cattccataa cttcgttgtt ataaacaaat
    12421 cttctctatc gacgccatta tcctttaatg ctcgtcctag tgaagcctca ttatcataaa
    12481 agtatgctgt atcaaacgct ctatagcctg cgtcaattgc agcatttaca actttagtca
    12541 tatcttcgtc agagatttta taaacaccta acccaactga aggcatcggg tatccattat
    12601 ttaatatttg tatctcattc aacatcttta tatctctcca atctatgtat cttttatatc
    12661 tttacattac cctaaatttt tcaacaaact caattaatac gaattatcgc tttcaataaa
    12721 aattattcat taaatcatta aagatattga gttccaatac tattttcact tttcattaat
    12781 tttagtcaaa aaaataacca accaaaaatg aattaaatca ttcttagttg gtttatatat
    12841 taatatctat tctgattttt catcttcatc agactgtccg atagtaggtc tcgcttcatt
    12901 aaattcatag tttaatatca cccaaataat ttggtggtta tcgatttctg aaacaaccca
    12961 gcgatcataa gttgtatcca cgtaatcatc tttttgtaaa ttggtattac gagattgtaa
    13021 ccatccacct atcgtatcaa tatcctcaga gtcatcaaat tctataccga actcttcagt
    13081 taaatcatcc aatagtactc tgccatttac ttggaatgtc ttattatcaa ttttaacgat
    13141 atcattcact tcatcatcat caaattcatc acgaatttct ccaacgattt cttctaaaat
    13201 atcttccatc gttaaaatac ctgccgttcc accatattca tctataataa gactcatatg
    13261 tacatgttca cgttgcattc taattaatgc atcactgata cgtgttgtct ctgaaatcat
    13321 tggcaactca tgtatatagt ttgctatttt aatcgttttt ccagaagcgt attcagttaa
    13381 aaattctttg acgttaataa atcctttaat gtggtcttta tcaccatcat cagtaattgg
    13441 ataacgcgta aattgatgtt cttttattgt ttctagtaat tcgtctacat taaaaggttc
    13501 atttagtgta atcatttgag ttctaggtac cattatatct tttgcatgtc tttcatcgaa
    13561 tgaaaagata ttttgcatat atgccaattc agtttggttg atttctccac cattataact
    13621 attgttaata ataattttga tttcttcttc tgacattgca tcagtttggg catcaggatt
    13681 tacaccaaac attctaataa taacacgtgc agaaccattc atcagccaaa tcaatggttt
    13741 cataatgtta ccgaaataga acaatggtct tgcatatact aaagcaagct tttcagtatg
    13801 ttgaatagct atagatttag gcgctaattc accaagtact acatgcaaat acgtaacgat
    13861 tataaatgac actgcaaacg aaatcgtcgt cgttaatgca gttggtaaat tgattgcttc
    13921 aaatattggg tgtaatagct tttcaaacgt tggttcacca agccaaccta accctaaaga
    13981 tgttactgtt atacctaact gacaagcaga aagataataa tctagattag caatcatctt
    14041 ttttactatt ttagcaggtt tatttccttc atctgctagc tgttcaattc ttgttgctct
    14101 aatttttact aatgcaaatt ctgaaccaac aaatacagtg gttaatgcaa ttaatagaat
    14161 aaatataatc aaactaatta tggtcgaagt ttccaattaa ttccctattt ctagggattc
    14221 acctccatgt tatatgtgtc actcatgggt aacacgcatt caaatttatc actatgactt
    14281 aaattacaac tacatgttat cgccttccca tttagacacc cccagaaaaa aatgttattg
    14341 cttctatttt atcataatat aaagtgctta tgttaacaga ttaaatctat tgcatacatt
    14401 taattatgat tatttattag ctaaatgtgt gaaccttgcc ttgttttatt gtagatttta
    14461 cattcatatc atcatctaga ataacaagat ctgcatcctt atttacttta atactacctt
    14521 ttctatcatc gatacctaat gcaatggctt gatttaaact tgttactcgc cataaatgat
    14581 ctaatgtatc acctgtaaat gatattaagt tacgtaaccc atgattcatt tttaaaatac
    14641 taccagcaag cgcaccattt gcaagacgtg cttgttgcga ttgaacagtt actttttgtc
    14701 cacccaaatc atattctcct tcaggcatac cttttgcacg cattgcatcg gtaattaaat
    14761 aaaaacgttc attacctttc atacggtaag caattgcaac cgatgccgga tgagaatgag
    14821 tgccatcaac aatcatttcg gtatgtagag catcattcaa ccatgctgct ccaaaaacac
    14881 ctggttctct atgttggaat ggcgtcgctg cattatataa atgcgtgaca tgtttagctc
    14941 ctcgctcaac agcttcaact gcttcttcgt atgttgctac tgtatgacca attgaaaaaa
    15001 taatgtcatc tttatacgtt tcaagcgctt cttttgcacc ttcaacttca ggtgcaaacg
    15061 tcataatctt tattaatccg ttagcagtct cttgaaaatg tttaatttta tcgataaatg
    15121 ggcgtacaac gtattgcgga tgttgagcac caactttatt ttcagatata aatggtcctt
    15181 ctaaatgtat acctacaatt tccgctgcat tgtgaacatc ttgctccgct tcatatttag
    15241 caatatttgt aagtgcatta tctattttat cagtcgattg cgtcattgta gtggccaagt
    15301 atgatgtcgt cccttcagac aacaaatttt cggatagata ttttaagcca tcgtatgacc
    15361 catccattgc atcttgacca taaccaccat gaatatgtat atcaataaaa cctggtaata
    15421 catgatgacc tttagcatca atcacttgaa ttttatttgt cgtatcatta tcaattgctg
    15481 ctttatcatc cacttctcca attgcaacaa tctgtccatc tttcacatga atgtaaccat
    15541 tatcgatttt gccatcttca gtataaactt tgccgttata tataattaat tctgacacgt
    15601 ataaaccctt ctttatttat aaattttcac tttaagacac tcatatttta ctatatctgc
    15661 ttcgtttggc ataacttttt gcaaaatatt aaaaaagtct atacatttgt ggtgtctatt
    15721 tcttcattca tttatctaaa aataaaaaca ctttcatatc aattcataaa aactgaatta
    15781 atagaaagtg tttttacagc tttgtataag ctaccgttaa cacgatatat aaaactaaca
    15841 tatattcatt gaacatacaa cttaatatga agctctttgc taacaatcat tttacatcat
    15901 taaaactact cgtccattga ttttgaagct tcgatttctg tttcagttaa ctttggtttg
    15961 attaaaccgt aaattaatgc tgaaactaat gtgccaacta gaagtgcaat aagagtttga
    16021 agtaagtgtg caccatcagt accaacaatt acaataatac caccatgtgg cgcagtaatt
    16081 cgtgaaccta agcctaaagc aattgcgcca cctatacctg aaccaatcat cattgaagga
    16141 ataacacgta atggatcggc agctgcaaat ggaatcgcac cttctgtaat aaatgacata
    16201 cccatcacat agttagggat aattgaacca cgttgttctt ttgtaaattt acgtctaaaa
    16261 attaacatcg ctgtcgcaat tgctaacggt ggaatcatac caccaatcat tgcagctgta
    16321 attggtgctg cattaccttc aatcaacgca cctgttgcaa aaacatatgc cgctttgttg
    16381 aatggaccgc ccatatcaat cgccatcata gcgccaataa ctaaacctaa taatacaata
    16441 ttagaacctg ataaattgtt taatccatct aataacaaat gatttaacca agatgctggt
    16501 ggattaaagg cataaatcat caataagcct gtagccgtca ctgttaatag tggataaatt
    16561 aatgttggct ttaatccctc taatgcttgt ggtaacttac gtgtcatggc tttaacacct
    16621 tgtgttaagt aacctgctaa gaaacctgca ataataccac caataaatcc tgaaccacct
    16681 gaaattgcta acataccacc tacaagacct gaagcgaaac caggtttatc cgcaatacta
    16741 cgtgcaatga atccagataa aattggaatg attaacgcga atgcactttt actaccaatg
    16801 ttccaaagct gctctgcaaa cgcattgtac tctgagcttt ttggattaaa tgaatttgct
    16861 ccaaataaaa atacaattgc cattaaaata ccaccagata ttacaagtgg caacatgtta
    16921 gaaacaccgt tcattaagtg tttatagaat gctttacctg ggcttaattt ttcttgactg
    16981 tcatttgagt taccttttct ttgaccatca cgggcaacaa aaggtttacg acttgtatct
    17041 aatgctttat taattaattc ttctgggcgt ttaataccat ctgctactgg tacttctacg
    17101 acatttttac catcgaagcg atccgtctca acatgaacat cagcagcaac aatgatacct
    17161 gttgcatttt caatatcttg ttcagttaaa tggtttttaa tgccgcttga accattcgtt
    17221 tctactttaa ttttaatacc cattttatcc gcttgctttt tcaatgcatc acgtgccata
    17281 tatgtgtgtg caatacctgt tggacaagca gttactgcta acacatatgg ctcattagat
    17341 tgtttagatg attgttctgc agttgcaact tgttgtgctt cagcttctgc ctcttcttct
    17401 tttgtcactt catcatcagc ctcatctatg atcgctagta cttcttcagg tgatgaagca
    17461 tgtaataatt tctcacgtac attttcatcc attaaaatac cagacaactt agctaaagca
    17521 tctagatgtg tttgggcgcc accttctggc gctgcaatca taaagaataa gtgtgctggt
    17581 tgcatatcca aactttgata atctacgcct gctttagatt taccaaacgc aatagctggt
    17641 gacttaactg cggccacttt ggcatgtgga atggcaatac cttcgccgat accagttgta
    17701 ctttgtgatt ctcgattgtg aatcgcttcc ttaaatgacg cgacatcact taatttacct
    17761 gctttgtcta attgatttac taactcatca ataacaccat ttttgtcatt tgccattaaa
    17821 tccattgcta ttgtatcttt tgttaataac tctgttactc tcattatttt cactccccat
    17881 caagtacgct aatcgtaact tgtgatttta ttttttctat agcgtcccgt gttgctaagt
    17941 cctcatcaaa tgccgtggca gtaccgcatg cgactgcttg ttggaatgct ttttcaatcg
    18001 ttaaacctga agcaattcca gccaccatgc ctgcaactgt actatcacca gagccaactg
    18061 tattaaccac tttcccttgt ggattaactg ctttaatact gatttcttta tcaatataaa
    18121 tagcaccatc accgccaagc gagacaataa cagattgcgc acctttatca actaacaaac
    18181 gaccatattt aataacatct gtgtctgagt tcactgttgt attaaacatc acttctaatt
    18241 catctttatt aggtttaata aatagtggat gatatggtaa aacgctttca gccaattctt
    18301 tttcagcgtc gactactaat ttagcacctg tctgtgctgt aatttgtgca atttgcgcat
    18361 acgcatcgct tggaatacta cttggtacac ttccagcaac aataactata tcttcgcttg
    18421 ttgtattttt aatttgttgt aacagttgtt caaattgtgt tgacgttata tgaggacccg
    18481 gtgcattgat ttctgtttct tgtcctgttt ttaatttcac attaatacgt gtatcttcat
    18541 caacttcaat aaaattcgat tgaattgcac tgttatttaa tgtatctata atgaatttcc
    18601 caggaaatcc acctgcaaat cccaaggcag ttgactcaac atccaatgtc tttaagacgc
    18661 gcgagacatt aatacctttc cccccagcga atttatatgt tgctgttgct ctgttcaaac
    18721 catcaatttt aaaatcattc gtaaaaatga catagtcaat tgaaggattg aaagtcactg
    18781 tataaatcat aaagtccctc ctataaagtg atacttttgt tggtattctt ttaacgattc
    18841 ttgatttaat gctttttcag atgtgatgat tgtcgtactt tctagcaaag gtacacgagc
    18901 aaaatatact ttattaaact tagaatgatc tataagtaca aatgattgat tggctaatga
    18961 cattgctgtt tgtttaacta atgcctcttg ctcatcggga gtagttaatc caagttcaat
    19021 atctaatcca ttcatcccga taaaagcttt atcgaaacaa tatcgtctta atatctccat
    19081 agcactagaa ccaatcgtag caagtgtatt ttctttaact tgaccaccta gcataattgt
    19141 tttaatacct tttttaagta aagcttctac atgtgttaaa ccattagtta ccacaatgat
    19201 atctttcgct tgaatatatt taattagctc caatgtagat gaaccagcat cgataaataa
    19261 gcattcatta tcgttgattt gattagctgc tattttagca atcattttct tttcatcaag
    19321 attcgttgct aatttttcag ttaaattcgc ctcaaccata cgattttctt ttaacattgc
    19381 accaccatgc acacgttgca gtttccctaa ttgttgtagt ttagataaat ctcttcgtat
    19441 tgttgaagca ctgcaaccag ttcgatctat taattcttgt aaagtcaaaa aatctttgtg
    19501 cgaaagttct tctaatatta actcgtgtct tttttcagta attatcatca tcaccccatc
    19561 tcactttgta ttataatgag tacgctttca cttttcaatc ataaattacc aaaaacaatc
    19621 acaaacactc aaattgtgac gataaaatta catttttatg ttcaaatact caattataat
    19681 gactgtttat gatcgtaaaa tgctaattca atcaaaaagg agtgtggcag aaatgaattt
    19741 tataaaaatt tcatctctgt cacactccta atcttgcccc atactttttg ttgttattaa
    19801 tcattcatgg ataactgctc caattttgcc tttagttatt gtaatcaaat cctcaacagc
    19861 tattgtgatt tgcattgttc gaagcccacc actcacactg atatgactat aattttcaca
    19921 cgatttgtca acgactgttg gaaacaatgt tttcatacca acaggcgaac acccaccacg
    19981 aatgtatccc gttacatttt tcaaattatc taaaggcata agctgcaatt tcttctctcc
    20041 aaccaaagca gctgcctttt tcatatctaa agtttcactt actgggataa caaatacaaa
    20101 atgttcatgt tttgtatttt ctagaacaag cgttttaaat actgttttaa catttgcttg
    20161 tatgagttct gcgacttctt gaccagataa atgttcttct ggtacctcaa agctatgaac
    20221 ttcgtattta atttttgcac gatcaagcat acgcatcgcg ttcgttttct tatttttagc
    20281 catttcaaca cctcatttat acttcttcag aactatatta acattttaaa atacattacg
    20341 gattggttga ttttgacata acatttacat cattttataa gttgaattca cctttttaaa
    20401 ttaaattatt gcaaaataaa aacagtatat ccaattagaa tacactgttc catctattat
    20461 ttcgttatta tgccttgatt tgaaatactt acatgttgct tactagttga attatcattg
    20521 gaatacacat aattatatcc atgatttgtc ttagaaattc tttctaaacc ataattatca
    20581 ttgatatgct tatctgcata acgatcagca ataacttggc actcttgttc gttaaaagaa
    20641 tttgatttat ttgcattcac acgatgttta aaatgatttt gctttttcac attttcattc
    20701 acttcagatg ctttagcagt atgaatattg agaatatcca ttttcttttt ataagtatct
    20761 tcagttgata catttatcgc gttaagcgta ttaattgttt tatgtacctt agcaagcgca
    20821 tcttcatttg ttgctaatat taataaacca attaaacaaa cggttaatat tacagcaaca
    20881 aagcctaaaa ataaattttt tactgtcatc cccttaccca catttcctta ctcatttttt
    20941 atcatcttaa cacattaacg ttaatgaatt aggtatgtgg attgcaattt aaaaagaatg
    21001 taatatttgt aacatgttta ttactcaaaa tcattttttg ttacatataa catttaaata
    21061 taaagagtaa aacatttagt caatcgaatc tttttaaatg taaagcatcg aattttctta
    21121 ataaaattca taagaaaaac gatgctaatc attcataata ttacgcttta ctttatatca
    21181 atatacacca aaatacttat gctacatatt ttgttctttc aattttgctc tatgttgctt
    21241 ttcaattaaa acaataacaa cacctgctaa tgaaacacct atagccatat aaattggtgc
    21301 ttcaatgtgt acatcaaata acgcacctgc gattaaagga cctatgaaat tacccatact
    21361 agtgaatgtc gagttcaatc cgcctgcaaa gccttgcctt tctccagcaa tattagaaaa
    21421 ataatttgta atggctggtc gtatcatatc aaaacctatg aagacaacaa aactgattaa
    21481 cattattgac caatagtcat tagcaaaaac taataatatt aagacaacaa ctgaatataa
    21541 taatgaccaa gctataaatg ttaactctga gaaatacttc ataaatttat cgaagaaata
    21601 gatttggaaa agtgccccaa atataccgcc acccgtaata gcaatcgaaa tatctttagg
    21661 tgaataattt accttgtcag ctgtgtatag tgaatacaat gtttcaaatg cagataaacc
    21721 aaacgataat acaagtgtta aaataactgg tgtaataaac actttccagt taattttcgt
    21781 tagcaattgt ggctctaact tttggaaacc acttgtcgta gactttttcg gatcgtgaat
    21841 caatacaatt gacattataa atgctagaat acctaatgct cctgcaaagt aaaatggcat
    21901 acgatgtgaa acttctgcca taaatccacc aatccctggt cctaaaatga atccagaatt
    21961 gataatcgct gacatgtagc caaagttttt tgctttttga tggcttggtg aaatgtcagc
    22021 tattaaacct gtcacaccag gcattaccat accagcactc ataccaccaa tcactctcga
    22081 taacatcaat accgaaaaat tgtggccaac tgcaaacata aattctgaca ctgaaaacaa
    22141 aattaatcct atacatataa ttaatttctt ccctaatttg tcagctagcg taccaccaaa
    22201 cggcgatata atcatttgag ataacgcaaa agcagcaact agtaatccta aatcactacc
    22261 agttaatccc aaatctttta aataaacagg caagactggt attactaaac cgatacctaa
    22321 aaaaatcaag aaaatattaa aatataagac aaaaatctgt ttattcatat gctcacctct
    22381 tttttctcca tgtcatgctt aaagctgtta ttcttctttt ccacgacaga ttgcaattca
    22441 tgctaaatat tcgtaaaaag tttctatatt gtattacaac attgctacat ttgacaatat
    22501 tttttctttg taattatcac tattttccat ttaattgtat aattaatgac atattaaaat
    22561 aaaggggatt gatcatatga aattgcaaaa aagtaatcat actatactac tagtgttgga
    22621 aaaaggagaa gatatcgttg aatgtataac aacctttgca gatgaccaag acttaacatt
    22681 tacatctgtc agcggcattg gtgcatgtga tgacgttgta ttaaaatttt tcaatttaac
    22741 aacgaaacaa tacgaagaaa aacatattac tgagccactg gaattgacga gcttattagg
    22801 taatatttct cgtctagaca atggtcattt tgcacatttg catgctactt tcggtactca
    22861 atcatatgaa acttttagcg gacatctcgc taaagcaatt gtttctgcaa ctgctgaaat
    22921 cattcttact gtaactgatt tggacattca acgttcattc aaagatgcag tcggtttaaa
    22981 tttacttgat cctcaatagc actttacttt ttagttgaat caaattttat tacaagttat
    23041 cataagtgct taatcataaa tgcattctcg tttattaata tacttcaacg agttaaatta
    23101 aatttttaat taggaattga atgcgtttat aactatcaca tttgaatgaa taactttttc
    23161 attagaaagt agtgtttatt attgatgcaa tctcgataca tcgctagaat ttacttttta
    23221 attttattta tcgtctccct ttttgagact aaaatttttc aatttatgac tttaaatttg
    23281 tttttagcat atataccttt tgagcttagt ctattattac gattattcaa accgaataaa
    23341 aagtatgaat ggccactctt tatcatatat agttttatat ttgtgttact tttaccaaat
    23401 acattttata tggtaactga tttaatccat ttgaaccaat ttcaattcaa cttttacgca
    23461 ggactaaatt taagtgagtg gaaatacttt acctatttac tgcttggtgt cttcttagcg
    23521 gtttatgtga tgattttaat ctattttgaa atactaacat tgacttctca ccgttggctt
    23581 aatagattaa ttgttgtcgt gctaatgttt ttaaatggat tcggaattta tattggtcgt
    23641 ttcttacgct tacattccgt ttatttcttt aatgagccct tacatgttct aaatcacatt
    23701 ctagaaagtc taacgcttaa aactgctatg tttgtatgtt ttatggttgt catgcaagca
    23761 gcaattctat tatttggaaa aggagtgcgt ttgaacaagt gacaataaat ataatcgttt
    23821 taatagtatc tatcattgtc tttcaattga ttattggaca catatggcat gatattggtt
    23881 taagttattt aagaagtatc ttactcatga tgctgccatt tggactaggt gtatttatac
    23941 aacaagtgtc gtattacgaa cgacaatatc ctaaatggca agtgcctcaa aacataaaag
    24001 ttagattgaa atatatttat ctcgctacgt ttttagagta tgtcgtacta tacctcactt
    24061 tatttacgga catactaaga taacatcaaa ttaataagaa aactaaatac aacttatata
    24121 tttgaggaaa gccaatttct atcacatcaa taatagaaat tggcttattt atattgctta
    24181 agaactttat attctgctcc agattttact catgccagat ctatcaattg agccgaatta
    24241 gtctagcgct ttaaatgtag ctaaaacttg tgtacgtttt tcttgatgat ctaccatttg
    24301 tcttggataa tgactaccta attcaatccc ctgttcaaaa aggttggact cattgcgttg
    24361 tgtatcatgc aaatattttg catcaatttg attaaaaatc ggaagatatg ttttgatata
    24421 caaagcttta gcatcaaagc gttcactctg tcttattgga ttaaacattc taaaatacgg
    24481 cactgcatcc gtacctgtag aagcagacca ttgccatcca tgaatatttg atgctgcatc
    24541 atagtcaata aggtgctttc taaagaattt ttctccccat gtccaatcta taaataaatc
    24601 tttggttaaa aattgcgaca caaccattct cattcgatta tgcataaaac cagtttgtgt
    24661 caattccatt attgctgcat caatgattgg aaatcctgtt tgcccttcgc accatgcatt
    24721 aaaatccgct tcattttgcg accattttat ctgtcgatat ttaggtttga aagcttggta
    24781 tgaggtttca ggatactgtg tcattaacac ataataaaat tctctaaaaa tgagttcacg
    24841 tataaatgct tcatagtttg cctcatcttc atcataacgc gccaataaat cattaaaaat
    24901 ttcaataata tctaataatc catatgctaa agcaacactt agctgacttg ttaatacttc
    24961 aggtaaatag tctcttccgc tttcgtaatt ctgtatatct tgatctaaaa aatgttgcca
    25021 tttattctgt tcaattattt gtgaggatcc aaatgctatg tcatcaaaca ctaaatcatc
    25081 aggtgatgca atgacaaagt cttcgaatga ttttaaatca tagtgatata cgtctctatg
    25141 cctcaagtaa ggtcgccatt ttttatagaa actagtaaaa acattgtatg gctccccttg
    25201 ttgattcatt gttttactcg ctttaaagta atgattccca cgtatcaaag taacggcaat
    25261 gcgtgcttca ttgaataaag aacgttgatg taaaatatca tattcttcaa gatgataact
    25321 cataatatca cccgcagtca ctacttcaga gatattgttt tctttacaaa atgaaatgat
    25381 atcgtcataa gacataacat aaggtgtaag cttttctgcg tgcaacgatt ggtaaaagcg
    25441 ctgcaaagta ccgcgataaa actgtgcttt caaatcagaa gctgaagaca tgtcctcttc
    25501 cggaatgata aaataacatg catcaatgtc ttctttttgt tgataaatat attcaaataa
    25561 tggattgtgt tccattcgaa acattcgatt taataacaca gcaattgcca taaacaaaac
    25621 acccttcaat tatcttttta ttttattaag ctttttgtca ctttaaaata acctagattc
    25681 ttatttatat ttttaattaa agcaaaccta accatttcca ataagtcatg ctaaaaacta
    25741 tcactagaat ataccctaca attgtcaatg gtatacctgc cttcaagaaa tctttaaccg
    25801 taaaagtacc agtgccataa gccaacatat tttgaggtgc actaacaggt aataagaaac
    25861 caaaactaat aacaaattgt tgaattaaaa caaatcctat agactggtct cctaagtgta
    25921 acgtagaggt tagcgaaata aaaacaggta ttaacgctga tgataaactt gttgcactcg
    25981 caaagcccaa atgaatcaat atattaaaaa gcgtgataag cgcaattgtc gcgataatag
    26041 gtaaatgttt taaacctaaa acaccaaaag tttgatcact taaccattga gctgcacctg
    26101 ttttcaaaag aacgttacct agtgaaatac ctacaccaaa cacgataatt gttccccatg
    26161 gtattttatt ttcaacatgt ttccatgtca tgacaccaat tttcggcatt aacataacac
    26221 ctaaagcaat aatagtaatg gatgcagagt caatcggatg taatactttt tcagttgacc
    26281 aaaacagtaa taataacatc gatataacaa ttaaacgcca ttcacgtggg ctaacggggc
    26341 caagtttatg caattcttct tttattaaat ctttaccacc ttctattgta ttaatttctg
    26401 gaggcatcac tttaatcatg atgaaatata aagctacgga cataactatg gaccaaggcg
    26461 ctgcatataa gaaccactcg ccccatgaaa catcaaatcc taattgatgg tttataaaat
    26521 taatcgctac gatattttgt gctgccgccg ttttgatacc aatattccaa attgacacag
    26581 cttgtactga agtaattatt aataaagacg ctaacttgct atctttggaa actttaaatg
    26641 ccgcaatcat acccagcaag attggtacaa ctgcccctgc tctagctgtt gcagaaggaa
    26701 cgaaaaatgc aagtacaatt gaaacgataa ttgctccaat aactatattt ctagttttat
    26761 taccaacaat tgataacact aaaagagcta gtcttttatg caaattcgtt tcttgcatag
    26821 cagcagccaa aaataatgca gctgcaacga gagctacagc tgaagttgca aatccactaa
    26881 acgctaatga taatgcatga ttagttccta gaaggtcact tccagctaaa atagcactgc
    26941 cacttttcgg attacctagc ttctccccta aattttgaac agggctaaat cctaaaagta
    27001 atatcattaa gccaataatt aaagttgctg acaccggata tgatacagct tccgttaccc
    27061 acataataac tgcaaaagct aaaatagcta gtactgcctt agccattatc ggcagacttg
    27121 aaggtgccgg gagtaatagt acaactataa gtattataaa actcatgaca atccaaaatg
    27181 gcttgtattg cttttttgtc ttttctttct tttgttgttc atgttcaact acatgttttt
    27241 cttctgacat aacagtcacc cctt
//
LOCUS       DALAGD010000031        25920 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.031, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000031 DALAGD010000000
VERSION     DALAGD010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 25920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25920
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.031"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(<1..860)
                     /gene="folD"
                     /locus_tag="O2701_001057"
     CDS             complement(<1..860)
                     /gene="folD"
                     /locus_tag="O2701_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCZ4292962.1"
                     /translation="MVAKILDGKQIAKDYRQGLQDQVEALKEKGFTPKLSVILVGNDG
                     ASQSYVRSKKKAAEKIGMISEIVHLEETATEEEVLNELNRLNNDDSVSGILVQVPLPK
                     QVSEQKILEAINPEKDVDGFHPINIGKLYIDEQTFVPCTPLGIMEILKHADIDLEGKN
                     AVVIGRSHIVGQPVSKLLLQKNASVTILHSRSKDMASYLKDADVIVSAVGKPGLVTKD
                     VVKEGAVIIDVGNTPDENGKLKGDVDYDAVKEIAGAITPVPGGVGPLTITMVLNNTLL
                     AEKMRRGIDS"
     gene            1061..1543
                     /gene="purE"
                     /locus_tag="O2701_001058"
     CDS             1061..1543
                     /gene="purE"
                     /locus_tag="O2701_001058"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCZ4292963.1"
                     /translation="MKVAVIMGSSSDWKIMQESCNMLDYFEIPYEKQVVSAHRTPKMM
                     VQFASEARERGINIIIAGAGGAAHLPGMVASLTTLPVIGVPIETKSLKGIDSLLSIVQ
                     MPGGIPVATTAIGAAGAKNAGILAARMLSIQNPSLVEKLNQYESSLIQKVEDMQNELQ
                     "
     gene            1530..2654
                     /gene="purK"
                     /locus_tag="O2701_001059"
     CDS             1530..2654
                     /gene="purK"
                     /locus_tag="O2701_001059"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCZ4292964.1"
                     /translation="MNFNKLKFGATIGIIGGGQLGKMMAQSAQKMGYKVVVLDPSEDC
                     PCRYVAHEFIQAKYDDEKALNQLGQKCDVITYEFENISAQQLKLLCEKYNIPQGYQAI
                     QLLQDRLTEKETLKSAGTKVVPFISVKESTDIDKAIETLGYPFIVKTRFGGYDGKGQV
                     LINNEKDLQEGFKLIETSECVAEKYLNIKKEVSLTVTRGNNNQITFFPLQENEHRNQI
                     LFKTIVPARIDKTAEAKEQVNKIIQSIHFIGTFTVEFFIDSNNQLYVNEIAPRPHNSG
                     HYSIEACDYSQFDTHILAVTGQSLPNSIELLKPAVMMNLLGKDLDLLENEFNEHPEWH
                     LHIYGKSERKDSRKMGHMTVLTNDVNQTEQDMYAKFEGSN"
     gene            2658..3362
                     /locus_tag="O2701_001060"
     CDS             2658..3362
                     /locus_tag="O2701_001060"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCZ4292965.1"
                     /translation="MTLLYEGKAKRIFSTNQENELRVEYKDEVTAGNGAKKDTMAGKG
                     RLNNQITSIIFKYLQENGIESHFIKQLSETEQLVKPVKIIPLEVVVRNIASGSITKRL
                     GFENGEVFREPLVEFFYKNDALNDPLITDDHVKLLNIASDEDIEILKSKALKINNVLK
                     QLMDAMNLKLVDFKIEFGKTETGQILLADEISPDTCRIWDKATNANFDKDVYRNNTGS
                     LIETYQIFLNKLEDLK"
     gene            3362..3625
                     /gene="purS"
                     /locus_tag="O2701_001061"
     CDS             3362..3625
                     /gene="purS"
                     /locus_tag="O2701_001061"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HCZ4292966.1"
                     /translation="MKTIELHITLQPQVLDTQGQTLTRAVHDLGYAQVNDIRVGKVLY
                     MTVDEVSDEKVHNIITTLSEKLFANTVIEEYSYKVLDDEKENA"
     gene            3627..4298
                     /gene="purQ"
                     /locus_tag="O2701_001062"
     CDS             3627..4298
                     /gene="purQ"
                     /locus_tag="O2701_001062"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HCZ4292967.1"
                     /translation="MKFAVLVFPGSNCDRDMFNAAIKSGVEAEYVDYRETSLSGFDGV
                     LIPGGFSFGDYLRSGAMASVAPIISEVKRLAAEGKPVLGVCNGFQILTEIGLLPGALL
                     HNDSHLFISRNEELEIVNNQTAFTNLYEQGEKVIYPVAHGEGHYYCTDEIYQQLKANN
                     QIILKYVNNPNGSYDDIAGIVNEKGNVCGMMPHPERALETLLGTDSGVKLFEAMVKSW
                     REQHV"
     gene            4291..6480
                     /gene="purL"
                     /locus_tag="O2701_001063"
     CDS             4291..6480
                     /gene="purL"
                     /locus_tag="O2701_001063"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HCZ4292968.1"
                     /translation="MSKFIEPSVEEIKLEKVYQDMGLSDQEYEKVCDILGRQPNFTET
                     GIFSVMWSEHCSYKHSKPFLKQFPTSGDHVLMGPGEGAGVVDIGDNQAVVFKVESHNH
                     PSAIEPYQGAATGVGGIIRDIVSIGARPINLLNSLRFGELDNKQNQRLLKGVVKGIGG
                     YGNCIGIPTTAGEIEFDERYDGNPLVNAMCVGVINHDMIQKGTAKGVGNSVIYVGLKT
                     GRDGIHGATFASEELTEESESKRPSVQIGDPFVGKKLMEATLEAITFDELVGIQDMGA
                     AGLTSSSSEMAAKGGSGLHLRLEQVPTREPGISPYEMMLSETQERMLLVVEKGTEQKF
                     LDLFDKHELDSAVIGEVTDTNRFVLTYDDEVYADIPVEPLADEAPVYILEGEEKDYNT
                     SKNDYTHIDVKDTFFKLLKHPTIASKHYLYDQYDQQVGANTIIKPGLQASVVRVEGTN
                     KAIASTIDGEARYVYNNPYEGGKMVVAEAYRNLIAVGATPLAMTDCLNYGSPEKKEIY
                     QQLIDSTKGMAEACDILKTPVVSGNVSLYNETKGTSIFPTPVVGMVGLIENVNYLNDF
                     EPQVGDKLYLIGDTKDDFGGSQLEKLIYGKVNHEFESLDLSSEVEKGESIKTAIREGL
                     LSHVQTVGKGGLLITLAKLSAHYGLGLKSSIDITNAQLFSETQGRYVVSVKSGKTLNI
                     DNAIEIGLLTDSDNFKVTTPYTEISENVSDIKQIWEGAIAQCLTTQD"
     gene            6459..7943
                     /locus_tag="O2701_001064"
     CDS             6459..7943
                     /locus_tag="O2701_001064"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCZ4292969.1"
                     /translation="MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIV
                     VSDQNELKGERGLGLLTEAIKDDQMERLKGYQHAIGHVRYATSGNKGIENIQPFLYHF
                     YDMSVGICHNGNLINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKES
                     LRKVKGGFTFAILTKDALYGAVDPNAIRPLVVGKMKDGTYILASETCAIDVLGAEFVQ
                     DIHAGEYVVINDKGITVKSYTHHTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKK
                     LAQESPVNADMVIGVPNSSLSAASGYAEEIGLPYEMGLVKNQYVARTFIQPTQELREQ
                     GVRVKLSAVKDIVDGKNIILVDDSIVRGTTIRRIVKMLKDSGANKVHVRIASPEFMFP
                     SFYGIDVSTTAELISASKSPEEIKDYIGADSLAYLSVDGLIESIGLDYDAPYSGLCVE
                     SFTGDYPAGLYDYEANYKAHLSHRQKQYISKNKHFFDSEGNLNV"
     gene            7936..8964
                     /locus_tag="O2701_001065"
     CDS             7936..8964
                     /locus_tag="O2701_001065"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCZ4292970.1"
                     /translation="MSKAYEQSGVNIHAGYEAVERMSSHVKRTMRKEVIGGLGGFGAT
                     FDLSQLNMTAPVLVSGTDGVGTKLKLAIDYGKHDSIGIDAVAMCVNDILTTGAEPLYF
                     LDYIATNKVVPEVIEQIVKGISDACVETNTALIGGETAEMGEMYHEGEYDVAGFAVGA
                     VEKDDYVDGSEVKEGQVVIGLASSGIHSNGYSLVRKLINESGIDLASNFDNRPFIDVF
                     LEPTKLYVKPVLALKKEVSIKAMNHITGGGFYENIPRALPAGYAARIDTTSFPTPKIF
                     DWLQQQGNIDTNEMYNIFNMGIGYTVIVDEKDVSRALKILAEQNVEAYQIGHIVKNES
                     TAIELLGV"
     gene            8967..9533
                     /gene="purN"
                     /locus_tag="O2701_001066"
     CDS             8967..9533
                     /gene="purN"
                     /locus_tag="O2701_001066"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCZ4292971.1"
                     /translation="MVKIAIFASGSGSNFENIVEHVESGKLENIEVTALYTDHQNAFC
                     IDRAKKHDIPVYINEPKQFDSKAAYEQHLVTLLNEDKVEWIILAGYMRLIGPDLLASF
                     EGKILNIHPSLLPKYKGIDAIGQAYHSGDTITGSTVHYVDSGMDTGEIIEQRKCDIRP
                     DDSKEQLEEKVKKLEYELYPSVIAKIVK"
     gene            9548..11026
                     /gene="purH"
                     /locus_tag="O2701_001067"
     CDS             9548..11026
                     /gene="purH"
                     /locus_tag="O2701_001067"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCZ4292972.1"
                     /translation="MKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVP
                     VRSVSDLTHFPEIMDGRVKTLHPAVHGGILADRNKPQHLNELSEQHIDLIDMVVVNLY
                     PFQQTVANPDVTMDEAIENIDIGGPTMLRAAAKNYKHVTTIVHPADYQEVLTRLRNDS
                     LDESYRQSLMIKVFEHTAEYDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIA
                     GAKQLHGKQLSYNNIKDADATLALVKKFDTPATVAVKHMNPCGVGIGDTIEQAFQHAY
                     EADSQSIFGGIVALNRAVTPELAEQLHSIFLEVIIAPKFTDEALDILKQKKNVRLLEI
                     DMTIDSNEEEFVSVSGGYLVQDKDNYVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPS
                     VKSNAIILSNNKQTVGIGAGQMNRVGAAKIALERAIEINDHVALVSDGFFPMGDTVEL
                     AAQHGIKAIIQPSGSIKDQDSIDMANKHGIAMVVTGTRHFKH"
     gene            11048..12295
                     /locus_tag="O2701_001068"
     CDS             11048..12295
                     /locus_tag="O2701_001068"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCZ4292973.1"
                     /translation="MNVLVIGAGGREHALAYKLNQSNLVKQVFVIPGNEAMTPIAEVH
                     TEISESDHQAILDFAKRQNVDWVVIGPEQPLIDGLADILRANGFKVFGPNKQAAQIEG
                     SKLFAKKIMEKYNIPTADYKEVERKKDALTYIENCELPVVVKKDGLAAGKGVIIADTI
                     EAARSAIEIMYGDEEEGTVVFETFLEGEEFSLMTFVNGDLAVPFDCIAQDHKRAFDHD
                     EGPNTGGMGAYCPVPHISDDVLKLTNETIAQPIAKAMLNEGYQFFGVLYIGAILTKDG
                     PKVIEFNARFGDPEAQVLLSRMESDLMQHIIDLDEGKRTEFKWKNESIVGVMLASKGY
                     PDAYEKGHKVSGFDLNENYFVSGLKKQGDTFVTSGGRVILAIGKGDNVQDAQRDAYKK
                     VSQIQSDHLFYRHDIANKALQLK"
     gene            complement(12560..13366)
                     /locus_tag="O2701_001069"
     CDS             complement(12560..13366)
                     /locus_tag="O2701_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCZ4292974.1"
                     /translation="MYEQWKVRQSFVDDVNIITKLFLGIILFFFIIFVHNFDFMLYIT
                     ILMLVFLLMFNGTQFKITAIFITISIGFALLSSLFMILYGNGSHTILKFGFIHITTES
                     LVRGLHVSLRTIAISFFGILIALTSQVVMIFYSLMQHLKVKSKVAYAFMAAIRMIPLM
                     ISSLIQLRRSLKMRYQMIDAANYRGLKRFKHLIIPLLSQNIRKAHQLSVAMESKGFRD
                     GPRTYYYNVPFSYKDIIFIIVLIAIITMAYYCSITLPITGIDDVRLGRVG"
     gene            complement(13359..14759)
                     /locus_tag="O2701_001070"
     CDS             complement(13359..14759)
                     /locus_tag="O2701_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4292975.1"
                     /translation="MLKVSDLRLKYPSGQRKIFDHLNITIQDKEKVLLLGPSGCGKST
                     LLNVLSGIVPNLIELPMKYDELIVDPLSGVIFQDPDSQFCMPKVYEELAFVLENRQLP
                     REDMDALIINALNMVNLNVTPETYIKDLSGGMKQKLAIVETILQQSKTLFLDEPTAML
                     DVQATEDLWTKLIELWEDQTVVIVEHKVKHIWNHVDRVILMDYNGNIIADECPEIILQ
                     KYVHLLSEYGVWHPRAWEFAPSRVDFPTTNSHLLQFKNGRIIRGKSTLLSFSDLEIGL
                     GEWITITGANGSGKTTLLESIMQLIKYQGDVYFENQRLTKIKHAAKHMYLVYQNPELQ
                     FITNSVYDEINIHFNHLSKDQSDDETIQLLKLLDLQNVKDQHPYELSIGQKRRLSVAT
                     ALSSKADIIFLDEPTFGLDSHNTFQLIKLFQKRINLGQSIVMVTHDDEIIERYPSRRL
                     KISDGALLDCDGDTNV"
     gene            complement(14774..15349)
                     /locus_tag="O2701_001071"
     CDS             complement(14774..15349)
                     /locus_tag="O2701_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCZ4292976.1"
                     /translation="MSKGLKLSEILVTVLISVVFAVIYNLWWFVYNGVQATGLHLEQL
                     TNGVWFMAAIVCYLIIPKPGIALLAEFAAGAGETIIMGRFDIPTIVYAFIQGLACELV
                     FAIFKYQSRSVMVAMLAGFCTAIAAFPIDYFYGYLNEVAGWNLTLFIVFRLISGAVVA
                     GVLSYLLVKALDKTGVTKLFRPAAKEDYDNL"
     regulatory      complement(15426..15526)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15933..16067)
                     /gene="graF"
                     /locus_tag="O2701_001072"
     CDS             complement(15933..16067)
                     /gene="graF"
                     /locus_tag="O2701_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance-associated protein GraF"
                     /protein_id="HCZ4292977.1"
                     /translation="MSNENQNKKAAEKAKEVEEKLKDKKEEKTEDINQTKQDIQDTLN
                     "
     gene            16219..17514
                     /locus_tag="O2701_001073"
     CDS             16219..17514
                     /locus_tag="O2701_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4292978.1"
                     /translation="MSFLRKHAEIIFSYLIGIVSLFTGLIILINLPLIKQLNGGKKVD
                     THVHNVWEFLNAFFSEIIKVMSRFIGDFPIVSAIVIIIFGILVMLIGHTLLRTIKYDY
                     DISIFFLVIGIMYFIITLILMTQVYGFFAVIFIIPFTIHIGYIVYKDELNQENVKNHF
                     MWIIVSYGISYLITQIALYGRIDANEIESIDILSVNAFFIIMWLLGQMAIWNFLFLRR
                     ALPLTKQELGEEEPELSRTSKGNVTNQTKIHLKQLQDKTTEYARKTRRSVDLDKIRAK
                     RDKFKKKVNDIIDIQEDDIPDWMRKPKWVKPMYVELFCGVVIFLFTFLEFNNRNALFV
                     SGDWKLSQTQYVIEWVTLLILLFIIIAYIATTLTFHLKGKFYYLQLFMGSILFFKLLT
                     EFINIMIHGLLLSVFITPTLLLMLLAIIISYSLQLRERP"
     gene            17939..19111
                     /locus_tag="O2701_001074"
     CDS             17939..19111
                     /locus_tag="O2701_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HCZ4292979.1"
                     /translation="MKIATLNKGKETKYFNGYPLIEEEDIYSQDHLKEGDIFQIVTDK
                     SQYVATAYVGRQHKGLGWVLTYDKAQQINTAFFVKLFNTALAERDYYFNIDGTNAFRL
                     FNAEGDGVGGLTIDNYDGHLLIQWYSKGIYKFKYAILEAVRKVFDYKSIYEKVRFKDS
                     EYSGGFVEGDAPEFPIVIEENFTFYNVDLEDGLMTGIFLDQKEVRKKLRDQYAKERHV
                     LNLFSYTGAFSAIAASEASSTTSVDLANRSRSLTEENFGLNAIDPKSQYIYVMDTFDF
                     YKYAARHGHSYDTIVIDPPSFARNKKRTFSVQKDYDKLINGALNILSSEGTLLLCTNA
                     SVYPLKQFKNTIKKTLEESGVDYELTEVMGLPKDFKTHPHYKPSKYLKAVFVNIRH"
     gene            19165..19707
                     /locus_tag="O2701_001075"
     CDS             19165..19707
                     /locus_tag="O2701_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HCZ4292980.1"
                     /translation="MGFKNNLTSNLTNKIGNSVFKIENVDGKGAMPTTIQELRERRQR
                     AEAIVKRKSLMSSTMSVVPIPGLDFGVDLKLMKDIIEDVNKIYGLDHKQVNSLGDDVK
                     ERIMSAAAIQGSQFIGKRISNAFLKIVIRDVAKRTAAKQTKWFPVVGQAVSASISYYF
                     MNKIGKDHIQKCENVIKNVM"
     gene            19861..20127
                     /locus_tag="O2701_001076"
     CDS             19861..20127
                     /locus_tag="O2701_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HCZ4292981.1"
                     /translation="MEQNSYVIIDETGIHARPATMLVQTASKFDSDIQLEYNGKKVNL
                     KSIMGVMSLGVGKDAEITIYADGSDESDAIQAISDVLSKEGLTK"
     gene            20130..21848
                     /gene="ptsP"
                     /locus_tag="O2701_001077"
     CDS             20130..21848
                     /gene="ptsP"
                     /locus_tag="O2701_001077"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCZ4292982.1"
                     /translation="MSKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAK
                     FNSAIEASKVELTKIRNNAEVQLGADKAAIFDAHLLVLDDPELIQPIQDKIKNENANA
                     ATALTDVTTQFVTIFESMDNEYMKERAADIRDVSKRVLSHILGVELPNPSMIDESVVI
                     VGNDLTPSDTAQLNKEFVQGFATNIGGRTSHSAIMSRSLEIPAIVGTKSITQEVKQGD
                     MIIVDGLNGDVIVNPTEDELIAYQDKRERYFADKKELQKLRDADTVTVDGVHAELAAN
                     IGTPNDLPGVIENGAQGIGLYRTEFLYMGRDQMPTEEEQFEAYKEVLEAMGGKRVVVR
                     TLDIGGDKELSYLNLPEEMNPFLGYRAIRLCLAQQDIFRPQLRALLRASVYGKLNIMF
                     PMVATINEFREAKAILLEEKENLKNEGHDISDDIELGIMVEIPATAALADVFAKEVDF
                     FSIGTNDLIQYTLAADRMSERVSYLYQPYNPSILRLVKQVIEASHKEGKWTGMCGEMA
                     GDETAIPLLLGLGLDEFSMSATSILKARRQINGLSKNEMTELANRAVDCATQEEVIEL
                     VNNYVK"
     gene            complement(22085..22318)
                     /gene="nrdH"
                     /locus_tag="O2701_001078"
     CDS             complement(22085..22318)
                     /gene="nrdH"
                     /locus_tag="O2701_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCZ4292983.1"
                     /translation="MSEIIVYTQNDCPPCTFVKNYLNEHHIDFEERNINNQQYRNEMI
                     DFDAFSTPFILLNGNPMYHVDLDEINKVLNIQD"
     gene            22516..23877
                     /locus_tag="O2701_001079"
     CDS             22516..23877
                     /locus_tag="O2701_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCZ4292984.1"
                     /translation="MDTVEISRFLTAMTLAVHIIFATIGVGMPLMFAIAEFLGIRKND
                     LQYIAMAKRWAKAYTITVAVGVVTGTIIGLQLSLIWPTFMEMGGHVIALPLFMETFAF
                     FFEAIFLSIYLYTWDRFKNKWTHFLISIPVIIGGSFSAFFITSVNSFMNTPAGFELKN
                     GKMVNVQPIEAMFNPSFIVRSFHVITTAGMTMAFVIASIAAFKLLRNRQPKDTVYHKK
                     ALKMSMIVGFFSTLLSMLAGDLSAKFLHKFQPEKLAAYEWHFDTSSHAKLLLFGVLDE
                     KTQQVKGAIELPGLLSFLADNSVKTKVQGLNDFPKSLHPPMIVHYFFDLMVTMGILCF
                     VISGVYVLTLMFKKLRKFSTHKWMLYGILLTGPASMLAIEFGWFLTEMGRQPWIVRGY
                     MRVAEAATQAGGITFVTILFGILYIILMYTCAYVLIRMFKNKPAYEDVNRLAKKQGGE
                     IEK"
     gene            23874..24893
                     /locus_tag="O2701_001080"
     CDS             23874..24893
                     /locus_tag="O2701_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCZ4292985.1"
                     /translation="MIYAFIGITVLWLFLFCYIIIASIDFGAGFFALHSKLTGDEKKI
                     NHLISRYLNPVWEVTNVFFVFFFVGFVGFFPESIKYLGTVLLIPGSIALIMISLRNSF
                     YAFENYGQDTKLAWMIMYGVSGLLIPASLSTALTITEGGYINVRNNVIDLDWVQLLLS
                     PFAWSVVFLAIISVLYISSGFLTYYAKKANDEPAYNLTRQWHIFLGPPMIIICLFVFL
                     SLRIQNSEHFYSAVFDYWWMFGISFLFFALASLLTFFKKKHGLAFVFVILQMMFAFFG
                     YGISKLPYLLYPFVKITDAYVNPEMGWTLVIVFILGLLLLLPSLILLLRLFVFDKEYV
                     EGKKS"
     gene            25026..25688
                     /locus_tag="O2701_001081"
     CDS             25026..25688
                     /locus_tag="O2701_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HCZ4292986.1"
                     /translation="MGKEYVVIGLGRFGGSIVRELNALDMDVMAIDHDENRVNEYSDI
                     ATHAVVADTTDEAVMKSLGIRNFDHVIVAIGENIQSSTLTTLILKELGVKKVTAKAQN
                     DYHAKILNKIGADTVVHPERDMGRRIAHNVASASVLDYLELADEHSIVELKATEKMAG
                     QSIIDLDIRAQYGINIIAIKRGKEFIISPNPNINLEIGDILIMIGHDNDLNRFEKNIA
                     TR"
BASE COUNT         8964 a         3717 c         4875 g         8364 t
ORIGIN      
        1 tacgaatcaa tacctcgacg cattttttct gcaagcaaag tattatttaa taccatagta
       61 attgttaatg gaccaacgcc accaggaact ggtgtaatag ctccagcaat ttctttaacc
      121 gcatcataat caacgtcacc ttttaatttg ccattttcat ctggcgtatt gccaacatcg
      181 ataattactg ctccttcttt gaccacatct tttgttacta aaccaggctt accaactgca
      241 ctgacaatga catcagcatc ttttaaatat gatgccatat cttttgaacg agaatgtaag
      301 attgttactg atgcattttt ttgaagtagt aacttagaaa ctggttgtcc gacaatatga
      361 cttcgtccaa ttacaactgc atttttacct tctaaatcaa tatcagcatg ttttaatatt
      421 tccatgatgc cgagcggtgt gcaaggtaca aaagtttgtt catcgatata taatttccct
      481 atatttattg gatgaaaacc gtccacatct ttttcaggat tgattgcttc taatattttc
      541 tgttcgctaa cttgttttgg taatggtact tgtaccaaaa taccacttac agaatcatca
      601 ttatttagtc tatttagttc gtttaatact tcttcttcag tagctgtttc ttccaaatgt
      661 acgatttctg aaatcatacc aattttttca gctgctttct tttttgatct aacataactt
      721 tgactagcgc catcattacc aactaatata acggataatt taggtgtaaa acccttttct
      781 tttagcgctt caacttgatc ttgtaacccc tgtctgtagt ctttggcaat ttgtttacca
      841 tctaaaattt tagcaaccat aaaaacttct cctcctattg tcaatatcct aactttgtta
      901 gtttataaaa ctaatttaca tagcaaaaga cgtaaattca aacaaaaaat caaaataaag
      961 ttcgattttt gattgaaaaa gcagaaattg cttgttatgc tatatctata atatacaact
     1021 aaagtaattc acaattcaaa cttttgaaag ggtgtacaaa gtgaaagtag cagtcattat
     1081 gggcagttct tccgattgga aaattatgca agagagttgt aacatgttgg attattttga
     1141 aattccgtac gaaaaacaag tagtatccgc acatcgtacg ccaaaaatga tggttcaatt
     1201 tgcttctgaa gcgagagaaa gaggtataaa cattatcatt gcaggcgctg gcggtgcggc
     1261 acatttacca ggtatggttg catcattaac gacgctacca gttattggag tgccgattga
     1321 aacaaaaagt ttaaagggta tagattcttt attatcaatt gttcaaatgc caggaggtat
     1381 tccggttgca acgactgcaa ttggtgcagc aggtgctaaa aacgcaggta tacttgcagc
     1441 aagaatgtta agtattcaaa atccttcttt agttgaaaaa ctaaatcagt atgaatcttc
     1501 gttaattcaa aaagtggagg acatgcaaaa tgaacttcaa taaattaaag tttggtgcga
     1561 ctattggcat tattggtggt ggtcagcttg gaaagatgat ggcacaatca gctcaaaaaa
     1621 tgggttataa agtggttgta ttggatcctt ctgaagattg tccatgtaga tacgttgcac
     1681 acgaatttat acaagccaag tatgacgatg aaaaggcact caatcaatta ggacaaaaat
     1741 gtgatgtgat tacttatgaa tttgaaaaca tttcagccca acaattaaaa ctattatgtg
     1801 aaaagtacaa tattccgcaa ggttaccaag ctatacagtt attacaagat cgcttaactg
     1861 aaaaagaaac attaaaaagt gctggtacca aagttgtccc gttcatttca gtaaaagaat
     1921 ctacagatat tgacaaagca attgaaacat taggatatcc ttttattgta aaaactagat
     1981 ttggtggcta cgatggcaaa ggtcaagttt taattaacaa cgaaaaagac ttacaagaag
     2041 gttttaaatt aattgaaact agtgaatgcg tagctgaaaa atatttgaat atcaagaaag
     2101 aagtatctct tactgttaca agaggaaaca acaatcaaat cacttttttc ccattacaag
     2161 aaaatgagca tagaaatcaa atacttttca aaacaattgt tccagcgaga atagataaaa
     2221 cagctgaggc gaaagagcaa gttaataaaa ttatccaatc gattcatttc attggaacat
     2281 ttacagttga attttttata gatagtaaca accaattgta tgtgaacgag atagcaccaa
     2341 ggcctcacaa ttccggacat tattcaattg aagcatgtga ttattcacaa tttgatactc
     2401 atattttagc agttaccgga caatcattac caaattcaat tgaattatta aagccagcag
     2461 tcatgatgaa cttactaggt aaagatttag atttattgga aaatgaattt aatgaacatc
     2521 cagagtggca cttacatatt tatggtaagt ctgagcgtaa agatagcaga aaaatggggc
     2581 atatgactgt actaacgaat gatgtaaacc aaactgaaca agatatgtac gctaaatttg
     2641 aggggagtaa ttaaagcatg acattattat atgaaggaaa agcgaagcgc attttctcaa
     2701 caaatcaaga aaatgaatta agagttgaat ataaagatga agttactgct ggaaacgggg
     2761 ctaagaaaga cacaatggca ggtaaggggc gattaaataa tcaaattact tctattatat
     2821 ttaaatattt acaagaaaat ggaatagaaa gtcactttat taaacaatta tctgaaacag
     2881 aacaattagt taagcctgtg aaaataattc cattagaagt agttgttcgt aatattgcta
     2941 gtggatctat tacaaagcgt ttaggttttg aaaatggtga agtttttaga gaaccacttg
     3001 tagaattttt ctataaaaat gatgcgttaa atgatccgtt gataacggat gaccatgtta
     3061 aattgctcaa tatagcatca gatgaagata ttgaaatact aaaatccaaa gcattaaaga
     3121 ttaataatgt gttgaaacaa ttaatggatg ctatgaattt aaaattagta gattttaaaa
     3181 tcgaatttgg aaagactgag actggtcaaa ttttgttagc ggatgaaata tctccagata
     3241 catgtcgaat ttgggataaa gctaccaatg caaactttga taaagatgta tatagaaata
     3301 acactggatc actgattgaa acatatcaaa tatttttaaa caaattggag gatttaaaat
     3361 aatgaaaaca attgaactac atatcacatt acaaccacaa gtattagata cgcaaggaca
     3421 aacgcttact cgagctgtac atgacttagg ttatgcacaa gtgaatgata ttcgtgtagg
     3481 aaaagtatta tatatgacag tggatgaggt tagtgatgaa aaggtacaca acattattac
     3541 aactctaagt gaaaaattgt ttgcaaatac agtgattgaa gaatatagct ataaagtgtt
     3601 agatgatgaa aaggagaatg cataaaatga aatttgcggt tcttgttttt ccaggttcga
     3661 attgtgatag agacatgttt aatgctgcta ttaaaagtgg tgttgaagcg gaatatgtag
     3721 attatagaga aacatcacta agtggatttg atggcgtact tattcctggt ggattttcat
     3781 tcggggatta cttaagatct ggggcaatgg ctagtgtagc gccgattatt tcggaagtta
     3841 aacgtcttgc agctgaaggt aagccagtat taggtgtttg taatgggttt caaattttaa
     3901 ctgaaatagg cttattacct ggtgcattat tgcataacga ttcacattta tttattagta
     3961 gaaatgaaga gttagaaata gtgaataatc aaacggcatt tacaaatctt tatgaacaag
     4021 gtgaaaaagt tatatatcct gtagctcacg gtgaaggtca ttattattgt actgatgaaa
     4081 tatatcaaca attaaaagct aacaatcaaa ttattctgaa atatgtgaat aatccgaacg
     4141 gttcatatga tgatattgca ggaattgtta acgaaaaagg caatgtatgt ggcatgatgc
     4201 cacatcctga aagagcttta gaaacgttgt taggtactga tagtggtgtg aaattatttg
     4261 aagcgatggt aaaaagttgg agggaacaac atgtctaaat ttatcgaacc aagcgttgaa
     4321 gaaattaaac ttgaaaaagt atatcaagat atgggattaa gtgatcaaga atatgaaaaa
     4381 gtttgcgata ttttaggcag acaacctaac tttacagaaa caggtatctt ttctgttatg
     4441 tggagtgaac attgctctta taaacattct aaaccgtttt taaagcaatt tcctacgtca
     4501 ggtgaccatg tgcttatggg gcctggtgaa ggtgcagggg tagtcgatat aggtgataat
     4561 caagccgtag tatttaaagt agagtctcac aatcatccat cagcaattga accatatcaa
     4621 ggggctgcta caggcgttgg tggaatcatt cgtgacattg tctctattgg ggctagacct
     4681 attaatttgt taaacagtct tagatttgga gaattagata ataaacaaaa ccaaagatta
     4741 cttaaaggtg ttgtaaaggg tatcggaggt tatggtaact gcattggtat tccaacaact
     4801 gctggtgaaa tcgaatttga tgaacgttat gatggcaatc cacttgttaa tgcaatgtgt
     4861 gttggtgtta tcaatcacga catgattcaa aaaggcacag caaaaggtgt aggtaattcg
     4921 gtcatttatg ttggtttgaa aactggtcga gatggtattc atggtgctac ttttgcatct
     4981 gaagaattga cggaagaaag cgaaagtaaa cgaccttctg tacaaatcgg tgatccattt
     5041 gtaggtaaaa aattaatgga agcaacactt gaagcaatta catttgatga attagttggt
     5101 attcaagata tgggtgctgc tggtttaaca tcttcatcgt ctgaaatggc ggcaaaaggt
     5161 ggtagtgggt tacatttgag attagaacaa gtgccaacac gtgagccagg tatttctcct
     5221 tatgaaatga tgctttcaga aactcaagaa cgtatgttac tagttgttga aaaaggtact
     5281 gaacaaaaat tcttagattt atttgataag cacgaattgg atagtgctgt tataggtgaa
     5341 gttacagata caaatcgttt tgttttaaca tatgatgacg aagtttatgc tgacattcca
     5401 gttgaaccac tagctgatga agcacctgta tatattttag aaggagaaga aaaagattat
     5461 aatacttcta aaaatgatta tacacacatc gatgttaaag atactttctt taaattactt
     5521 aagcatccga ctatagcatc taaacactat ttatatgatc aatacgacca acaagttggt
     5581 gccaatacga taattaagcc aggacttcaa gcatcggtag tacgtgtgga aggcacaaat
     5641 aaggcaattg cttcaacaat tgatggtgaa gcgcgttatg tatataacaa tccatatgaa
     5701 ggtggaaaga tggtagtagc tgaagcttat cgaaatttaa ttgccgtggg tgcaacacca
     5761 ttagcaatga cagattgttt aaattatggt tctcctgaaa agaaagaaat ctatcaacag
     5821 ttgatagatt caacgaaagg tatggcagaa gcatgcgaca ttcttaagac accagtagtt
     5881 tctggtaatg tatctttata taacgaaacg aaaggtactt ctattttccc aacaccagtt
     5941 gttggaatgg taggtttgat tgaaaatgta aattatttaa atgattttga acctcaagtt
     6001 ggagataaat tatatttaat cggtgatact aaggacgact ttggtggtag tcaacttgaa
     6061 aagttaattt atggcaaagt taatcatgaa tttgagtcat tagatttgag ttcagaagtt
     6121 gaaaaaggtg aatcaatcaa gaccgctatt cgtgaaggac tattatcaca tgttcaaaca
     6181 gttggtaaag gtggcttact gattacctta gctaaactaa gtgcgcatta cggtttagga
     6241 ttaaaatctt caatagatat aacaaatgca caattgttta gtgagacgca aggccgatat
     6301 gttgtttctg ttaaatcagg taaaacttta aatattgata atgcaataga aattggactt
     6361 ttaacagata gtgataattt caaggtaaca acaccatata cagagattag tgaaaatgtt
     6421 tcagatatta aacaaatatg ggaaggggca attgctcaat gtttaactac tcaggattaa
     6481 acgaagaatg tggcgtgttt ggtatttgga atcatcctga agcagcgcaa ctaacatata
     6541 tgggacttca tagtttgcaa catcgtggtc aagaaggtgc aggtatagtt gtttctgatc
     6601 aaaatgaatt aaaaggcgag cgaggattag gcttactaac tgaagcgatt aaagatgatc
     6661 aaatggaacg attaaaagga tatcaacatg caattggtca cgtccgttat gctacttcag
     6721 gtaataaagg tattgaaaat attcaaccgt ttctgtatca cttttatgat atgagtgtag
     6781 gtatttgtca taatggtaac ctcattaatg ctaaatcatt gcgtcagaat ttagaaaaac
     6841 aaggtgctat cttccattcg tcttctgata ctgaagtcat tatgcatttg atacgtcgaa
     6901 gtaaagctcc tacttttgag gaagcgttga aagaaagttt gcgaaaagtt aaaggcggtt
     6961 ttacatttgc gattttaact aaagatgcgt tatatggcgc agtagatcca aatgctatca
     7021 gaccacttgt tgtaggtaaa atgaaagatg ggacatacat ccttgcaagt gaaacatgtg
     7081 caatagatgt gttaggtgca gaatttgttc aagatattca tgcaggtgaa tatgtcgtga
     7141 ttaacgataa aggtattaca gttaaatctt atacacatca tacgacaact gcaatttctg
     7201 cgatggaata tatttatttt gctagaccag actcaacaat agctggtaaa aatgtccatg
     7261 cagtacgtaa agcttctggt aaaaaattag cccaagaaag ccctgtaaat gctgatatgg
     7321 tcatcggtgt acccaattca tcgctatcag ctgcgagtgg ttatgctgaa gaaataggtt
     7381 tgccatatga aatgggacta gttaaaaatc aatatgttgc aagaacattt attcaaccaa
     7441 ctcaagaatt acgtgagcaa ggtgtgagag tgaagttatc tgcggtaaaa gatatagtag
     7501 atgggaaaaa tatcattctt gttgatgatt ccattgttcg cggtacgaca attcgacgca
     7561 ttgtgaaaat gttaaaagat tctggtgcaa ataaagtgca tgtgcgtata gcatcaccgg
     7621 aatttatgtt tccaagtttt tacggaatcg atgtttcaac tacggcagaa ttaatttctg
     7681 caagcaaatc acctgaagaa attaaagatt atattggcgc tgattcatta gcatatctat
     7741 ctgtagatgg gttaattgaa tcaattggtt tagattatga cgcgccatat agtggcttat
     7801 gtgtagaaag tttcactgga gattatcctg cagggttata tgattatgaa gcaaattata
     7861 aagcgcattt aagtcatcga caaaagcaat atatttctaa aaacaaacac ttttttgata
     7921 gcgagggaaa tttaaatgtc taaagcatat gaacaatctg gtgtaaatat tcatgctggt
     7981 tatgaagctg tagaaagaat gtctagtcat gttaaacgta cgatgcgtaa agaagttatc
     8041 ggtggtttag gtggattcgg tgctacattt gatttatcac aattaaatat gacagcgcca
     8101 gttttagttt ctggaacaga cggagtaggt acgaaattaa aactagctat cgactatggt
     8161 aaacatgatt cgataggtat cgatgcagtc gcaatgtgtg ttaatgatat tttaacgaca
     8221 ggtgcagaac cattatattt tttagattac attgctacaa acaaagttgt tcctgaagtt
     8281 attgaacaaa ttgttaaagg tattagtgat gcatgtgttg aaacgaatac tgcacttatc
     8341 ggtggagaga ctgctgaaat gggtgaaatg tatcacgaag gcgaatatga tgtagctgga
     8401 tttgctgttg gagcagttga aaaggatgac tatgtagatg gttcagaagt gaaagaggga
     8461 caagttgtta tagggcttgc gtcaagtggc attcattcaa atggatacag tttagtgcgc
     8521 aaattaatta atgaatcagg cattgatttg gcatcaaact tcgataatcg tccatttatc
     8581 gatgtctttt tagaaccaac taaattatat gtcaaacctg tacttgcttt gaaaaaagaa
     8641 gtttctatta aggcaatgaa tcatattact ggtggaggtt tttatgaaaa tattccacgt
     8701 gcattgccag ccggatatgc tgctagaatt gatactacat catttccaac accaaaaata
     8761 tttgattggt tacaacaaca aggcaatata gacacaaatg aaatgtataa catttttaat
     8821 atgggtattg gctatacggt tatcgttgat gaaaaagatg tatcacgcgc tttgaagatt
     8881 ttagcagaac aaaatgtgga agcctatcaa attggtcata ttgtgaaaaa tgagtcaact
     8941 gcaattgaat tgttgggggt ataacaatgg ttaaaattgc gatttttgca tcaggttcag
     9001 gaagtaactt tgaaaatata gttgagcatg ttgaatcagg aaaacttgaa aatattgaag
     9061 ttacggcgct atatacggat catcaaaatg cgttttgtat agatagagca aaaaagcacg
     9121 atattcctgt ttatattaat gaaccaaaac aatttgattc aaaagcagcg tatgaacaac
     9181 atttagtaac attattaaat gaagataagg tagagtggat tattctagct ggctacatgc
     9241 gtctaattgg tccagactta ttagcttcat ttgaaggtaa aatattgaat atacatccat
     9301 ctctattgcc gaaatataag gggattgacg caataggcca agcatatcat agtggcgata
     9361 ctattactgg ttcgacagta cattatgttg atagtggtat ggatacggga gaaattattg
     9421 aacagagaaa atgtgatatt agaccggacg attcaaaaga acaattagaa gagaaagtaa
     9481 aaaaattgga atatgagtta tatccaagtg ttattgctaa aattgtaaaa taaggagtat
     9541 gtatgtgatg aagaaagcta ttttgagcgt atcaaataaa acaggtattg tagagtttgc
     9601 taaagcgtta acgcaattaa attatgaatt atattcaaca ggtggtacta aacgtatatt
     9661 agatgaagca aatgtaccag ttcgttctgt ttcagactta acacattttc cagaaataat
     9721 ggatggccgt gttaaaacat tacatccggc tgttcatggt ggcattttag ctgatcgaaa
     9781 taaaccgcag catttaaatg aattatcaga acaacatata gatttaattg atatggtagt
     9841 agttaattta tatccattcc aacaaactgt tgcaaaccct gatgtgacga tggacgaagc
     9901 aattgaaaat attgatattg gtggtccaac aatgttacgt gctgcagcta aaaactataa
     9961 acatgtaaca acaattgtac atccggcaga ttatcaagaa gtattgacgc gattaagaaa
    10021 cgattcgtta gatgagtcat atagacaatc attaatgatt aaagtttttg agcatactgc
    10081 agaatatgat gaagcgattg tacgtttctt taaaggggat aaagaaactt taagatatgg
    10141 agaaaatcca caacaatcag cgtattttgt gagaacttcg aatgctaagc acacgattgc
    10201 aggcgctaaa caattacatg ggaaacaatt aagctataac aatattaaag atgcagatgc
    10261 tacactagct ttagttaaaa agtttgatac acctgctaca gttgcggtta aacacatgaa
    10321 tccatgtggt gttggtatcg gtgacacgat agaacaagca tttcaacatg cgtatgaagc
    10381 ggatagtcaa tcaatatttg gtggaattgt tgcattaaac cgagctgtaa cacctgagtt
    10441 agcagagcaa ttgcatagta tctttttgga agtcattatt gcaccaaaat ttacagatga
    10501 agcattagat attttaaaac aaaagaagaa tgtaagatta ttagaaattg atatgactat
    10561 agacagtaac gaagaagagt ttgtttcagt atctggcgga tatttagttc aagataaaga
    10621 caattatgtc gtgccaaaag aagaaatgaa agttgttaca gaagtagcac ctacagatga
    10681 acaatgggaa gcaatgttat taggatggaa agttgtacca tcagtaaaaa gtaatgcaat
    10741 tattttaagt aataataaac aaactgtagg tataggtgct ggacaaatga atcgtgtcgg
    10801 tgctgctaaa attgcgttag agagagctat tgaaatcaat gatcatgtag cgttagtatc
    10861 tgatggattt ttccctatgg gagatacagt tgaacttgca gcacaacatg gtataaaggc
    10921 aattatccaa ccgagtggtt cgattaaaga tcaagattca attgatatgg ctaataaaca
    10981 tggtattgca atggtagtca caggcactcg acattttaaa cactaatttt aaaggggcgt
    11041 aacataaatg aatgtattag taattggtgc tggtggacga gaacatgcac ttgcatataa
    11101 acttaatcaa tcgaatctag ttaaacaagt gtttgtcatt ccaggtaatg aggcaatgac
    11161 acctatagct gaagtacaca ctgaaatttc agaatctgat catcaagcga tactagattt
    11221 tgctaaacgg caaaatgttg attgggtagt tataggtcca gaacagccgc taattgatgg
    11281 attagcagac attttacgag cgaatggttt caaagtgttt ggtccaaata agcaagcagc
    11341 tcaaatcgaa ggctcaaaat tatttgctaa aaagataatg gaaaaatata atattccaac
    11401 tgctgattat aaagaagttg agcgaaaaaa ggatgcttta acatatattg aaaactgtga
    11461 attgcccgtt gttgtcaaga aagatgggtt agctgctggg aaaggcgtta ttattgcaga
    11521 tactattgaa gcagccagaa gtgctattga gattatgtat ggtgatgaag aagaaggtac
    11581 tgttgtattt gaaacgtttt tagaaggtga agagttctcg ctaatgacat ttgttaatgg
    11641 tgatttagca gtacctttcg actgtattgc acaagatcat aaacgcgcat ttgatcatga
    11701 tgaaggacca aatactggtg gtatgggggc ttattgtcca gtaccacata ttagtgacga
    11761 tgttttaaaa cttacaaatg aaacaattgc acaacccatt gcaaaggcaa tgcttaatga
    11821 aggttatcaa ttcttcggtg tattatacat tggtgctatt ttaactaaag atggtccaaa
    11881 agtaatagaa tttaatgccc gttttggtga tcctgaagct caagtattat taagtcgcat
    11941 ggaaagtgat ttaatgcagc atattattga tttagatgaa ggaaaacgta ctgaattcaa
    12001 atggaaaaat gaatctattg taggggtcat gttggcatca aaaggatatc ctgatgcata
    12061 tgaaaaaggg cataaagtaa gtggctttga tttaaatgaa aactattttg ttagtggatt
    12121 aaagaagcaa ggtgatacct ttgttacttc aggtggtaga gttatacttg ccatcggaaa
    12181 aggtgacaat gtacaagatg cacagcgaga cgcatacaaa aaagtatcac aaatacaaag
    12241 tgaccattta ttctatcgtc atgacattgc gaataaagca ctacaactta aataagtaaa
    12301 tttaaaatac taagattagc tatgaacgaa tctataacga tagatttttt catagctttt
    12361 ttagttgtag agtctaggac attgatttct gtaccaaatt tgtgattatg catatgtaat
    12421 acaaaagagg cgccacaaca tgtttggatg aacaaaataa catgtttgtg gcacctcttt
    12481 tgtttagtat ggaataaatg gttttctttt tctatacaat gaatttctaa tttagtatct
    12541 atacaattat ggataaaatt taacctacac gaccaagacg aacatcatct atgcccgtga
    12601 tgggtaaggt gattgaacaa taatatgcca tagtaataat ggcaattaaa actataataa
    12661 agattatatc tttatatgag aaaggtacgt tgtaatagta agtacgagga ccatctctaa
    12721 atcctttcga ctccatcgca actgataatt gatgtgcctt tctaatattt tggcttaata
    12781 gaggtataat taaatgctta aatcgcttta accctctata atttgccgcg tctatcatct
    12841 gatagcgcat ttttaaagat ctgcgaagct gtattaaaga actaatcatt aaaggtatca
    12901 tacgaatggc agccatgaat gcataagcaa cttttgattt aacctttaaa tgttgcatta
    12961 aactataaaa tatcatgaca acctgagatg taagtgcgat taagataccg aaaaatgaaa
    13021 tagcaatggt tcttaatgat acatgtaaac cacgaactaa actttctgtt gtaatatgga
    13081 taaatccgaa tttcaaaatt gtatggctac cattcccgta taaaatcatg aacagggaag
    13141 agagtaatgc aaagccaata cttatagtta taaaaattgc tgtaatttta aactgagtac
    13201 cattaaacat caataagaaa actaacatta agatagtgat ataaagcata aaatcgaaat
    13261 tatgcacaaa tataataaag aaaaatagta taattccaag aaatagtttc gttataatgt
    13321 tgacatcatc aacaaatgat tgccgaactt tccattgctc atacattcgt atcaccatca
    13381 caatctagta acgcaccatc tgaaatttta agtcttcttg atggataacg ttcaattatt
    13441 tcatcgtcat gtgtaaccat gacaatactt tgtcccaaat taattcgctt ttggaaaagt
    13501 ttgatcaact ggaatgtatt atggctatca agtccaaatg tcggttcatc taaaaagata
    13561 atatcagctt tagaacttag tgcggtagct acgctaaggc gtcgtttttg accaatagac
    13621 aactcataag gatgttgatc ttttacattt tgtaaatcta aaagttttaa aagttgtatc
    13681 gtttcatcat cactttgatc tttagaaagg tgattaaaat gaatgttaat ttcatcataa
    13741 accgaatttg ttataaattg taattctggg ttttgataaa ctaggtacat gtgttttgct
    13801 gcatgtttaa tttttgttaa acgctgattt tcaaaataaa catcaccttg atatttaatc
    13861 aattgcataa ttgattcaag caaggttgtt ttaccactac catttgcccc tgtaattgta
    13921 atccactcac ctagaccaat ttctaaatct gagaatgaga gcaatgttga tttaccgcga
    13981 ataatacgtc catttttaaa ttgtaataag tgtgagtttg ttgttggaaa gtcaacacga
    14041 cttggtgcga attcccatgc acgtggatgc cacacaccat attcactgag taaatgaaca
    14101 tacttctgta atatgatttc aggacattca tcggcaatga tatttccgtt ataatccatc
    14161 aaaatgacgc ggtcgacatg attccagatg tgtttaactt tatgttcaac gattacaacc
    14221 gtttgatctt cccaaagttc aattagttta gtccataaat cttctgttgc ttgaacatct
    14281 aacattgctg tcggttcatc taaaaacaat gtttttgatt gttgaagaat ggtttcaaca
    14341 attgccaatt tctgtttcat cccgccactt aaatctttga tatacgtttc aggggtaaca
    14401 tttaaattga ccatatttaa agcattgata attaacgcat ccatgtcttc acgtggtaat
    14461 tgtctatttt ctaaaacgaa tgcaagttct tcgtatactt ttggcataca aaactggcta
    14521 tcagggtctt ggaaaataac gccacttaat gggtcaacga ttagttcatc atatttcata
    14581 ggtaattcaa ttaaattagg aacaatacca cttaatacat tcagaagtgt acttttaccg
    14641 caaccagaag gaccgagtaa aagtactttt tctttgtctt gaatagtgat atttaaatga
    14701 tcgaaaattt tacgttgacc acttggatat tttaatcgta aatcacttac ttttaacact
    14761 ctgaatgctc ctcttataag ttgtcgtaat cttctttagc agctggtcta aataattttg
    14821 ttacgcctgt cttatctaaa gcttttacta aaaggtaaga taggacgccg gcgactactg
    14881 caccactaat taatctaaat acgatgaata atgttaagtt ccaacctgca acttcattta
    14941 aataaccata gaaataatct atcgggaaag ccgcgattgc tgtacaaaaa cctgctaaca
    15001 tagctaccat aactgaacgt gattgatatt taaaaattgc aaagacaagt tcacacgcta
    15061 aaccttgtat aaaagcgtaa acgattgtcg gaatatcgaa acgacccata atgatagttt
    15121 cgccggcacc tgcagcaaat tcagccagta aagcaatacc tggttttgga ataattagat
    15181 agcagacaat cgctgccatg aaccaaaccc cgtttgttaa ttgttcgagg tgaaggcctg
    15241 tagcttgcac accattgtaa acaaaccacc ataaattgta aataactgcg aatactactg
    15301 aaataagtac ggttactagt atttcagata gctttaaacc ttttgacatt tttacatcct
    15361 cctaataaaa aaacgcacaa ccatccatag gaaagttatg cgttcacaat atatattagt
    15421 aaaacatatg tatagtaaca ctttcctacg ctagttcaag ctagatcagg ttcaaagggt
    15481 ttgagggcaa gcctcatctc agtataaaac acccctagtg tgtgcgattt atttaattaa
    15541 ttatactgta agacgtttgt aaacttatgt caataggttg tcttcatgaa atttcgttta
    15601 attcgattta aaatttataa tattagcatt ggatttaaat tgaagatgta gtaggaatgt
    15661 tagtaattaa agatataaaa atatgtgaca tgtaataata ttgagctgat aaatgaagag
    15721 ggatacttat caatcatacc tctttaacaa cagtgaagaa cccgtgcata atggcttacg
    15781 aattatagtt tataaggaag aagagggata catgcgccga gcacatgcat aaaagcccct
    15841 aacaactaaa agttgtaagg aaggagaggg atacatgcgc cgagcacatg cataaaatcc
    15901 cctaacaact aaaagttgta aggggattta aattaattta gtgtatcttg gatatcttgt
    15961 tttgtttgat taatatcttc tgttttttct tcttttttat cttttaattt ttcttcaact
    16021 tctttagctt tttctgctgc ttttttattt tgattttcat tagacatgat taattcctcc
    16081 caaattggat aattatttat atataaatct tacccggttg tactttcgtt aaacttttct
    16141 aagtctatag cactatttat tcatttatct aaagacaaca acattagatt aatatataat
    16201 gattttgagg tgaacataat gtcttttctt aggaaacacg ccgaaattat ttttagctat
    16261 ttaatcggta tcgtttcact cttcactggt ctcattattt taattaactt gccattaatt
    16321 aaacaattaa atggtggtaa aaaagttgat acacatgttc ataatgtgtg ggaatttctg
    16381 aatgcatttt tcagtgaaat tattaaagta atgagtcgat ttataggtga tttccctata
    16441 gttagtgcaa ttgtgataat tatattcggt attttagtta tgttgattgg tcatacatta
    16501 cttagaacta ttaagtatga ctatgatatt tctatctttt tcttagttat cggtatcatg
    16561 tactttatta ttactcttat attaatgact caagtttatg gattctttgc agtgattttc
    16621 attattccat ttacaattca tataggatat atcgtctata aagatgaatt gaatcaggaa
    16681 aatgtaaaaa atcatttcat gtggataatt gtgagttatg gtataagtta cttaattaca
    16741 caaattgcat tgtatggcag aattgatgct aatgaaatag agtcaattga tatcttaagt
    16801 gtcaatgctt tctttataat tatgtggtta cttggtcaaa tggctatttg gaatttcttg
    16861 ttcttgcgcc gagctttacc tttaacaaag caagaattag gtgaagagga gccagaatta
    16921 tcaagaacaa gtaaagggaa tgtcacgaat caaactaaaa ttcacttgaa acaactccaa
    16981 gataagacta cagaatatgc acgtaagaca agaagaagtg tcgatttaga taaaattaga
    17041 gctaaaagag ataaattcaa aaagaaagtt aatgatatta tcgatattca agaagacgat
    17101 attcctgatt ggatgagaaa accgaaatgg gttaaaccaa tgtatgtcga actattttgt
    17161 ggtgtcgtca tctttttatt cacattttta gaatttaata atcgtaatgc attatttgta
    17221 tctggtgatt ggaaattatc acagacacaa tatgttattg aatgggttac attattaatt
    17281 ctgttattca ttattatcgc atatatcgct acaacgttaa ctttccactt gaaaggtaag
    17341 ttttattatt tacaattatt tatggggagc attttattct ttaaattgtt aacggaattt
    17401 ataaatataa tgattcatgg actattactt tcagtgttca ttacgccaac attactatta
    17461 atgttattgg caatcatcat ttcttattcg ttacaattac gagagcgacc ataattaaaa
    17521 gcattataaa agtactatct attaaacatt ttgatgtgta cgctataagt tagatatatc
    17581 tctaacttac ttagatacag gtcaatgaag tttatggata gtactttttt tgtaactaga
    17641 tttgattgat tcaggtgatg tgaattaagt attgaaaatt gtatacaaag ttttagtgca
    17701 aataaaatag ttgaaaagtt atccatttgt aaaatcaaga aaactagtaa atagttgaag
    17761 cgacttatgg aatttgcgaa acgatatata gtatttcctt tgtagaaatt taacatatat
    17821 cattcgaatt actaatttgt taaaatcaac agtaagatta gaagtagatg atattgaaat
    17881 ttggcaaaca atttaatcta tataaaacta caacgaaaca cagaaaggaa gttgtcagat
    17941 gaaaatagca actctgaaca aaggcaaaga aacaaaatat tttaatggat atcctttaat
    18001 tgaagaagag gatatctatt cacaagatca tttaaaagaa ggagatattt ttcaaattgt
    18061 gactgataaa tcacaatatg tcgcaacggc ttatgttggt cgccaacata aaggattagg
    18121 ttgggttcta acatacgata aagctcaaca aatcaacaca gctttctttg taaaattgtt
    18181 taatactgca ttagcagaac gtgattatta ttttaatata gatggaacaa atgcttttag
    18241 attatttaat gctgaaggtg atggtgttgg gggattaaca atcgacaatt acgatggtca
    18301 tttgttgatt caatggtact caaaaggtat ttataaattt aaatatgcca ttcttgaagc
    18361 ggttagaaaa gtatttgatt ataaatctat ttacgaaaaa gtaagattta aagacagcga
    18421 atatagtggt ggttttgttg aaggagatgc acctgagttt ccaattgtta tcgaagaaaa
    18481 cttcacattt tataatgtag accttgaaga tggtttgatg acaggtatct ttttagatca
    18541 aaaagaagtg cgcaagaaat taagggatca atatgccaaa gaacgccatg ttttaaactt
    18601 atttagttat acaggtgctt tttctgcaat agcagcaagt gaggcatctt caacaacaag
    18661 tgtagatttg gctaatcgtt ctcgtagttt aactgaagaa aattttggat taaatgctat
    18721 tgatcctaaa tcccaatata tttatgtcat ggacactttt gatttctata aatatgctgc
    18781 acgacatgga catagttatg acacgatcgt gattgatcca cctagctttg cgcgtaacaa
    18841 aaaacgtaca ttttcagtgc aaaaagatta tgacaaatta attaatggcg ccttaaatat
    18901 cttatcatct gaaggaacat tattgttatg tacaaacgca agtgtatatc cattaaagca
    18961 atttaaaaat actattaaaa agacgcttga agagagtggc gttgattatg aattaactga
    19021 agttatggga ttaccaaaag attttaaaac gcatccacat tataagccat ctaaatattt
    19081 aaaagctgtt tttgtaaata ttagacatta atttgataaa aaggggtata gtaacttatt
    19141 gagaaaaaga agggtgataa tattatggga ttcaaaaaca atttaacatc aaatttaaca
    19201 aataaaatcg gtaattcagt ctttaaaata gaaaatgttg acggaaaagg tgcaatgcca
    19261 acgacgattc aagaattgag agaaagacga caacgtgctg aagcaattgt aaagagaaag
    19321 tctttaatgt catcaacaat gagcgttgtt ccaattccgg gtttagattt tggtgttgat
    19381 ttaaaattaa tgaaagatat tatcgaagat gttaataaaa tttatggttt agatcataag
    19441 caagttaata gccttgggga tgatgtgaaa gaaagaatta tgtctgcagc agcaattcaa
    19501 ggtagtcaat ttattggtaa aagaatttca aatgcatttt taaaaattgt aattagagat
    19561 gtagctaaac gtactgctgc aaaacaaaca aaatggtttc ctgttgtagg acaagctgtg
    19621 tctgcatcta ttagttacta ttttatgaat aaaattggaa aagatcacat tcaaaaatgc
    19681 gaaaatgtta ttaaaaatgt catgtaggtg ctataatagt tttgcaattt gcaaatttta
    19741 ctgaaaccgg ttttaaacga attgaattta aagcatggtt ttggtaaagt taatgtataa
    19801 aactaagtta gtattgtaat aatattgaag attctaacta tacgaaggag aaatgtaatt
    19861 atggaacaaa attcatatgt aatcatcgac gagactggta ttcacgctag accagcaaca
    19921 atgttagtac aaacagcttc aaaattcgat tctgatattc aattagaata taacggtaag
    19981 aaagtaaact taaaatcaat catgggtgtt atgagccttg gtgttggtaa agatgctgaa
    20041 attacaattt atgctgacgg tagtgatgaa tctgacgcca ttcaagcaat cagtgacgtc
    20101 ttatcaaaag aaggattgac taaataatca tgtctaaatt aattaaaggt attgccgcat
    20161 ctgatggtgt cgcaattgct aaagcttatt tattagttga gccagactta acattcgaca
    20221 aaaatgaaaa agtcactgat gttgaaggag aagttgcaaa gttcaatagc gctatcgaag
    20281 cttctaaagt tgagttaact aaaattagaa ataatgcaga ggttcaacta ggtgctgata
    20341 aagctgctat ctttgatgca catttattag ttttagatga ccctgaatta attcaaccaa
    20401 tccaagataa gattaaaaat gaaaacgcta atgctgctac agcattaacg gatgtaacaa
    20461 cacaatttgt tacaattttt gaatctatgg ataacgaata catgaaagaa cgtgcggctg
    20521 atattcgcga cgtttctaaa cgtgtgttat cacatatttt aggtgtagaa ttaccgaatc
    20581 cgagtatgat tgatgaaagc gttgttattg tagggaatga cttaacgcca tctgatactg
    20641 ctcaattaaa taaagaattc gtacaaggtt ttgctacaaa cattggcgga agaacaagtc
    20701 actctgcaat tatgagtcgt tctttagaaa ttccagcaat tgttggtaca aaatcaatta
    20761 ctcaagaagt taaacaaggc gacatgatta tcgtagatgg attaaatggt gatgtaatcg
    20821 ttaatccaac tgaagatgag ttaatcgctt atcaagataa acgtgagcgt tattttgctg
    20881 acaagaaaga attacaaaaa ctacgtgatg ctgatactgt tacagttgat ggtgttcacg
    20941 cagagcttgc tgcaaatatt ggtacaccta atgatttgcc aggtgttatt gaaaatggtg
    21001 cacaaggtat cggcttatat agaactgagt ttttatatat gggtcgtgac caaatgccta
    21061 cagaagaaga acaatttgaa gcttataaag aagtattaga agcaatgggc ggtaaacgtg
    21121 ttgttgtacg tactttagat ataggtggag ataaagaatt atcatactta aacttgcctg
    21181 aagaaatgaa tccattctta ggttaccgtg cgattcgttt atgccttgcg caacaagata
    21241 ttttcagacc acagctacgt gcattattac gtgcatcagt ttatggtaag ttaaatatca
    21301 tgttcccaat ggttgcaaca attaacgaat ttagagaagc taaagctata ttattagaag
    21361 aaaaagaaaa ccttaaaaat gaaggtcatg acatttcgga tgatatagaa ttaggaatca
    21421 tggtagagat acctgcaaca gcagcattag ctgatgtctt tgctaaagaa gtagatttct
    21481 tcagtatcgg tacaaatgat ttaattcaat acacattagc tgctgaccgt atgtcagagc
    21541 gtgtatcata tctataccaa ccatataacc cttcaatctt acgtttagtt aaacaagtta
    21601 ttgaagcgtc acataaagaa ggtaaatgga caggtatgtg tggtgaaatg gctggagatg
    21661 aaacagctat tccattattg cttggtttag gtttagatga gttctctatg agtgcaacgt
    21721 ctattctgaa agcaagaaga caaattaatg gtttaagtaa aaatgaaatg actgaacttg
    21781 ctaaccgtgc agtcgactgt gcaacgcaag aagaagttat tgaattagtt aacaactacg
    21841 taaaataatt aattctttcg aaagacagaa ttataaataa acccgaagta ttcattgtaa
    21901 gtaattatga atacttcggg ttctttttta tgatttatgt taatttcaat ggaatgttgt
    21961 tgctaactaa ttaaacaact atgtgtcatt aacaaaatgt agttctttaa ttaatgttag
    22021 ttaatttaaa aatcgatatt taatttatga tagctataaa tgatgaagtt gatttagatg
    22081 aaaattaatc ttggatattt aatactttgt tgatttcatc aagatcaaca tggtacattg
    22141 gattgccatt caacaaaata aacggagttg aaaaagcatc aaaatctatc atttcgtttc
    22201 gatattgttg attgttgata tttctctctt caaaatcaat gtgatgctca tttagataat
    22261 tttttacaaa tgtacaaggt ggacaatcat tctgcgtata aacgattatt tctgacatgt
    22321 atcatgctcc ttcgtgcttt attctaatgt aatatacaca attatacatt gaaattcaaa
    22381 tttgtgaaca cattgtgaac tgacataaat tatacacaat ttcatagcgc ataatgtttt
    22441 aaaaatacac attatagcaa ttataattaa gttcattgtg ttgtcatcaa aaaaagaaaa
    22501 aggtgatgtt tttaaatgga tacagttgaa atcagtcggt ttttgacagc tatgacttta
    22561 gcagttcata tcatttttgc aacgattggt gttggtatgc ctttaatgtt cgcaattgca
    22621 gaatttttag gtattcgcaa aaatgatctt caatatatag ctatggccaa aagatgggct
    22681 aaagcttata caattactgt agcagtggga gttgttacag gtacaattat aggacttcaa
    22741 ttatcattga tttggcctac atttatggaa atgggtggac acgttattgc acttccatta
    22801 tttatggaaa catttgcgtt cttctttgaa gctattttct taagtatata tttatatact
    22861 tgggatcgtt ttaaaaataa atggacacat ttcttaatta gtataccagt aattattggt
    22921 ggctctttct cagcattctt cattacttca gtgaattcat ttatgaatac gcctgcaggt
    22981 tttgagttga agaatggaaa gatggtcaat gttcaaccta tagaagcgat gtttaaccca
    23041 tcgtttatag ttcgatcatt tcacgtaatt actacagccg gtatgacgat ggcatttgtt
    23101 attgcatcaa tagcagcttt taaattattg cgtaatcgtc aacctaaaga tactgtctac
    23161 cataagaaag ctttgaaaat gtctatgata gttggattct tttcaacatt actttctatg
    23221 ttggcagggg atttatctgc aaaatttttg cataaattcc aacctgaaaa attagcagct
    23281 tatgaatggc atttcgatac atcttcccat gccaaattat tattatttgg tgtgttagat
    23341 gaaaagactc agcaagttaa aggtgcgatt gaattacctg gactattgag tttcttagca
    23401 gataatagtg tcaaaactaa agtacaaggg ttaaatgatt ttccaaaaag tttacatccg
    23461 cctatgatcg tccattattt ctttgattta atggtaacga tgggaatttt atgttttgtc
    23521 atttcaggtg tttatgtctt aactttaatg tttaaaaagc ttagaaagtt ttctactcat
    23581 aaatggatgc tttatggaat attattaaca ggcccagctt caatgctagc tatagaattt
    23641 ggatggttct taacagagat gggtagacag ccttggattg ttcgtggtta tatgcgcgtg
    23701 gcagaagcag caacacaagc aggcggaata accttcgtta caattttatt tggcatattg
    23761 tacatcattt taatgtatac atgtgcatac gtattaattc gtatgtttaa aaataaaccg
    23821 gcgtatgaag atgtaaatcg tttagccaag aagcaaggag gagaaataga aaaatgattt
    23881 atgcatttat aggtataaca gtgttatggt tgtttttatt ttgctatatc attattgctt
    23941 ctatagattt tggggcaggt ttcttcgcat tgcattcaaa gttaactggt gatgaaaaga
    24001 aaattaatca cttaatttca cgttatttaa acccagtttg ggaagttacg aatgtattct
    24061 ttgttttctt cttcgtagga ttcgtaggtt tctttccaga gtcaatcaaa tatttaggca
    24121 cggtattgtt aataccaggt tcaatagcac tgattatgat atcgttgaga aacagttttt
    24181 atgcatttga aaattatggt caagatacaa aattagcatg gatgatcatg tatggggtaa
    24241 gtggattatt aattccagct tcattatcta ctgctttaac tattacagaa ggtggctata
    24301 ttaatgttcg aaacaatgtt atcgatctag attgggtgca gttattatta agcccatttg
    24361 cttggtctgt agtattcttg gcaattattt cagttttata tatttcatca ggatttttga
    24421 catattatgc taaaaaagca aatgacgaac cagcatataa tttaacaaga caatggcaca
    24481 tatttttagg gccgccgatg attatcattt gtttattcgt atttctatca ttacgcattc
    24541 aaaattctga acatttttat tcagctgttt ttgactattg gtggatgttt ggtataagtt
    24601 tcttattctt tgcattagct tcattgttaa cattctttaa gaaaaaacat ggtttggctt
    24661 ttgtatttgt tattttacaa atgatgttcg cgttctttgg ctatggaatt agtaaattgc
    24721 catacctttt atatccgttt gtaaaaatta cagatgcata tgttaatcca gaaatgggct
    24781 ggacattagt gattgtcttt attttaggtt tacttttatt acttccatcg ttaatattat
    24841 tattaagatt atttgttttc gacaaagaat atgttgaagg aaagaaatca taagttgaga
    24901 cgatacccca actttcattt acttgcatta ctgcatttag ataatttagt tggggtactc
    24961 atttataata attttaaatt tatattttca atgaattgat gaaaatcatt taatggaggg
    25021 cgagtatggg taaagaatat gtagtcatcg gtctaggtcg ttttggaggt agtatcgttc
    25081 gtgagttgaa tgcattagac atggatgtaa tggccatcga ccatgatgaa aatagagtga
    25141 atgaatatag tgatatcgct actcatgcgg ttgttgcaga cactacagat gaagcagtta
    25201 tgaaaagttt aggtatccgt aactttgatc atgtcattgt ggcaattggt gaaaatattc
    25261 aatcaagtac gttgacgact ttaattttaa aagagttagg tgtaaaaaaa gtaactgcta
    25321 aagcacaaaa tgattatcat gcaaagattt taaataaaat tggagcagat acggttgtgc
    25381 accctgagcg tgatatgggt agacgtattg cgcataatgt tgcgagtgca agtgtacttg
    25441 attatcttga gttggcagac gagcattcta ttgtagaatt gaaagcaact gaaaagatgg
    25501 cggggcagtc tatcattgat ttagatataa gagcacaata tggaattaac attattgcaa
    25561 ttaaacgagg caaagagttc atcatttcac caaatccaaa tattaattta gaaataggtg
    25621 atattttaat catgattgga catgataatg atttaaatcg ctttgaaaaa aatattgcga
    25681 cgagataatc gggagtggga cagaaatgat attttctcaa aatttatttc gtcgtcccac
    25741 cccaacttac attgtctgta gaaattggga atccaatttc tctttgttgg ggcccacacc
    25801 ccaactcgca ttgcctgtag aatttctttt cgaaattctc tatgttgggg cccctgacta
    25861 gaattgaaaa aagcttgtta caagcgcatt ttcgttcagt caactactgc caatataact
//
LOCUS       DALAGD010000032        25601 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.032, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000032 DALAGD010000000
VERSION     DALAGD010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 25601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25601
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.032"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            29..697
                     /gene="capA"
                     /locus_tag="O2701_001082"
     CDS             29..697
                     /gene="capA"
                     /locus_tag="O2701_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     protein CapA"
                     /protein_id="HCZ4292987.1"
                     /translation="MESTLELTKIKEVLQKNLKILIILPLLFLIISAIVTFFVLSPKY
                     QANTQILVNQTKGDNPQFMAQEVQSNIQLVNTYKEIVKSPRILDEVSKDLNDKYSPSK
                     LSSMLTITNQENTQLINIQVKSGHKQDSEKIANSFAKVTSKQIPKIMSVDNVSILSKA
                     DGTAVKVAPKTVVNLIGAFFLGLVVALIYIFFKVIFDKRIKDEEDVEKELGLPVLGSI
                     QKFN"
     gene            713..1399
                     /gene="cap8B"
                     /locus_tag="O2701_001083"
     CDS             713..1399
                     /gene="cap8B"
                     /locus_tag="O2701_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8B"
                     /protein_id="HCZ4292988.1"
                     /translation="MSKKENTTTTLFVYEKPKSTISEKFRGIRSNIMFSKANGEVKRL
                     LVTSEKPGAGKSTVVSNVAITYAQAGYKTLVIDGDMRKPTQNYIFNEQNNNGLSSLII
                     GRTTMSEAITSTEIENLDLLTAGPVPPNPSELIGSERFKELVDLFNKRYDIIIVDTPP
                     VNTVTDAQLYARAIKDSLLVIDSEKNDKNEVKKAKALMEKAGSNILGVILNKTKVDKS
                     SSYYHYYGDE"
     gene            1402..2166
                     /gene="cap8C"
                     /locus_tag="O2701_001084"
     CDS             1402..2166
                     /gene="cap8C"
                     /locus_tag="O2701_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8C"
                     /protein_id="HCZ4292989.1"
                     /translation="MIDIHNHILPNIDDGPTNETEMMDLLKQATTQGVTEIIVTSHHL
                     HPRYTTPIEKVKSCLNHIESLEEVQALNLKFYYGQEIRITDQILNDIDRKVINGINDS
                     RYLLIEFPSNEVPHYTDQLFFELQSKGFVPIIAHPERNKAISQNLDILYDLINKGALS
                     QVTTASLAGISGKKIRKLAIQMIENNLTHFIGSDAHNTEIRPFLMKDLFNDKKLRDYY
                     EDMNGFISNAKLVVDDKKIPKRMPQQDYKQKRWFGL"
     gene            2186..4010
                     /gene="cap8D"
                     /locus_tag="O2701_001085"
                     /pseudo
     CDS             2186..4010
                     /gene="cap8D"
                     /locus_tag="O2701_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498717.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8D"
     gene            4000..5028
                     /gene="cap8E"
                     /locus_tag="O2701_001086"
     CDS             4000..5028
                     /gene="cap8E"
                     /locus_tag="O2701_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8E"
                     /protein_id="HCZ4292990.1"
                     /translation="MFDDKILLITGGTGSFGNAVMKQFLDSNIKEIRIFSRDEKKQDD
                     IRKKYNNSKLKFYIGDVRDSQSVETAMRDVYYVFHAAALKQVPSCEFFPVEAVKTNII
                     GTENVLQSAIHQNVKKVICLSTDKAAYPINAMGISKAMMEKVFVAKSRNIRSEQTLIC
                     GTRYGNVMASRGSVIPLFIDKIKAGEPLTITDPDMTRFLMSLEDAVELVVHAFKHAET
                     GDIMVQKAPSSTVGDLATALLELFEADNAIEIIGTRHGEKKAETLLTREEYAQCEDMG
                     DYFRVPADSRDLNYSNYVETGNEKITQSYEYNSDNTHILTVEEIKEKLLTLEYVRNEL
                     NDYKASMR"
     gene            5041..6150
                     /gene="cap8F"
                     /locus_tag="O2701_001087"
     CDS             5041..6150
                     /gene="cap8F"
                     /locus_tag="O2701_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8F"
                     /protein_id="HCZ4292991.1"
                     /translation="MNIVITGAKGFVGKNLKADLTSTTDHHIFEVHRQTKEEELESAL
                     LKADFVVHLAGVNRPEHDKEFSLGNVSYLDHVLDILTRNTKKPAILLSSSIQATQDNP
                     YGESKLQGEQLLREYAEEYGNTVYIYRWPNLFGKWCKPNYNSVIATFCYKIARNEEIQ
                     VNDRNVELTLNYVDDIVAEIKRAIEGTPTIENGVPTVPNVFKVTLGEIVDLLYKFKQS
                     RLDRTLPKLDNLFEKDLYSTYLSYLPSTDFSYPLLMNVDDRGSFTEFIKTPDRGQVSV
                     NISKPGITKGNHWHHTKNEKFLVVSGKGVIRFRHVNDDEIIEYYVSGDKLEVVDIPVG
                     YTHNIENLGDTDMVTIMWVNEMFDPNQPDTYFLEV"
     gene            6154..7278
                     /gene="cap8G"
                     /locus_tag="O2701_001088"
     CDS             6154..7278
                     /gene="cap8G"
                     /locus_tag="O2701_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8G"
                     /protein_id="HCZ4292992.1"
                     /translation="MEKLKLMTIVGTRPEIIRLSSTIKACDQYFNQILVHTGQNYDYT
                     LNQIFFDDLELRQPDHYLEAVGSNLGETMGNIIAKTYDVLLREQPDALLILGDTNSCL
                     AAVSAKRLKIPVFHMEAGNRCFDQNVPEEINRKIVDHVSDVNLPYTEHSRRYLLDEGL
                     NKANIFVTGSPMTEVIEAHRDKINHSDVLNKLGLEPQQYILVSAHREENIDNEKNFKS
                     LMNAINDIAKKYKMPVIYSTHPRSWKKIEESKFEFDPLVKQLKPFGFFDYNALQKDAF
                     VVLSDSGTLSEESSILKFPGVLIRTSTERPEVLDKGTVIVGGITYNNLIQSVELAREM
                     QNNNEPMIDAIDYKDTNVSTKVVKIIQSYKDIINRNTWRK"
     gene            7281..7907
                     /locus_tag="O2701_001089"
     CDS             7281..7907
                     /locus_tag="O2701_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HCZ4292993.1"
                     /translation="MRIAIEKIIGLLKNQSSKESNVKIHRLAYITNSKFDGNNYIDRW
                     CKIRNSHIGEYSYIGFGSDFNNVEVGRYCSISSDVKIGLGKHPTHFFSSSPIFYSNNN
                     PFNIKQKFIDFNDQPSRTTIKNDVWIGANVIIMDGLTINTGAVIAAGSVVTKNVGAYE
                     VVGGVPAKVIKKRFDNKTIEKLLESKWWEKTPDKLKGFSVEYLNKKDT"
     gene            7912..9021
                     /locus_tag="O2701_001090"
     CDS             7912..9021
                     /locus_tag="O2701_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCZ4292994.1"
                     /translation="MRILNIVSSNIVQDPRVLKQIETIKGVTDDYKIVGMNNSQATNK
                     RLENLDCNYRLLGSKVDPKNILSKLIKRIRFATGVIREIKAYKPDVIHANDFDVLLMV
                     YLSNYKKANIVYDAHEIYAKNAFINKVPLISKFVESIEKHIVKHRVNAFVTVSHAAKE
                     YYQSKGYKKEANVITNAPILNDSREFKEIENFKEIVYQGQIVMDRGYEEFIIASSAFK
                     QNAPSFIIRGFGPHEEVIKELISYNPENIRLDKPVEVKELVDKLAESNVGVVLTKPVS
                     INFEYTVSNKIFECIHAGLPVILSPVKEHIYLNEKYKFGIVLKEVTPLEIEKAVRKLR
                     DNHDLFNHLRQNAIKASKILNWQIESERLVELYKF"
     gene            9035..10201
                     /locus_tag="O2701_001091"
     CDS             9035..10201
                     /locus_tag="O2701_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCZ4292995.1"
                     /translation="MKFFVLCAIISMNIFIVISTFTKEVLGFPIEPVYYSTMVGIALI
                     TTVFAIYKIIVTQEIPRGLILLIAICLLYLAFYYFSPDKEEKLAKNNILFFLTWAVPA
                     AISGIYIKYINKATVERFFKLVFFIFSVSFIFVILIPKLTGEIPSYINFGLMNYQNAS
                     YLSAFTAGLGIYFIMKGSVKHKWIYVLFTIIDIPIVFIPGGRGGAILLILYGLFAFIL
                     ITFKRGIPIAVKSIMYIFALSISSVLIYFLFTKGSNTRTFSYLQGGTLNLEGTSGRGP
                     IYEKGIYFIQQSSLLGYGPFNYYKLIGNIPHNIIIELILSFGLLGFFIIMICILLLVY
                     KMIRNYDPNTIDLLVMFIAIYPITLLMFSSNYLVVSEFWFVLFYFITKGRRHHG"
     gene            10194..11399
                     /locus_tag="O2701_001092"
     CDS             10194..11399
                     /locus_tag="O2701_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HCZ4292996.1"
                     /translation="MAKKVFIMDSVKTIIGTLLIALGLQFLAYPIINQRVGNEAFGSI
                     LTIYTIITITSVVLGNTLNNIRLINMNLYKSNHYYWKFASILLISILIESIALIIVFL
                     YFFNLNIIDIIFLILLNILMCLRIYLNVFFRMTLKYNQILYIALIQFLGLLIGLFLYY
                     LTQNWIVCFITSELFATIYTLVKLRGLTIGEYQSEDNNVVKDYVMLLSTNSLNNLNLY
                     LDRLILLPIIGGTAVTISFLSTFIGKMLATFLYPINNVVLSYISVNESDNIKKQYLKT
                     NLIAIAALCLVMIICYPITIIIVSLLYNIDSSLYSKFIILGNIGVLFNAVSIMIQTLN
                     TKHASITLQANYMTLHTITFIFITILMTIAFGLNGFFWTTLFSNIIKYVILNIIGLKS
                     KFINKKDVD"
     gene            11400..12605
                     /gene="cap8L"
                     /locus_tag="O2701_001093"
     CDS             11400..12605
                     /gene="cap8L"
                     /locus_tag="O2701_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8L"
                     /protein_id="HCZ4292997.1"
                     /translation="MSEKKILILCQYFYPEYVSSATLPTQLAEDLIANHINVDVMCGW
                     PYEYSNHKQVSKTEMHRGIRIRRLKYSRFNNKSKVGRIINFFSLFSKFVINIPKMLKY
                     DQILVYSNPPILPLIPDVLHRLLKKKYSFVVYDIAPDNAIKTGATRPGSMIDKLMRYI
                     NRHVYKNAENVIVLGTEMKNYLLNHQISKNADNIHVIPNWYDMRQLQDNRIYNDTFKA
                     YREQYDKILLYSGNMGQLQDMETLISFLKLNKDQSQTLTILCGHGKKFADVKTAIEDH
                     RIENVKMFEFLTGTDYADVLKIADVCIASLIKEGVGLGVPSKNYGYLAAKKALVLIMD
                     KQSDIVQHVEQYDAGIQIDNGDAHAIYNFINTHSSKELHEMGERAHQLFKDKYTREIN
                     TMKYYNLLK"
     gene            12616..13173
                     /gene="cap8M"
                     /locus_tag="O2701_001094"
     CDS             12616..13173
                     /gene="cap8M"
                     /locus_tag="O2701_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8M"
                     /protein_id="HCZ4292998.1"
                     /translation="MKRLFDVVSSIYGLVVLSPILLITALLIKMESPGPAIFKQKRPT
                     INNELFNIYKFRSMKIDTPNVATDLMDSTSYITKTGKVIRKTSIDELPQLLNVLKGEM
                     SIVGPRPALYNQYELIEKRTKANVHTIRPGVTGLAQVMGRDDITDDQKVAYDHYYLTH
                     QSMMLDMYIIYKTIKNIVTSEGVHH"
     gene            13173..14060
                     /gene="capN"
                     /locus_tag="O2701_001095"
     CDS             13173..14060
                     /gene="capN"
                     /locus_tag="O2701_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     epimerase CapN"
                     /protein_id="HCZ4292999.1"
                     /translation="MRKNILITGVHGYIGNALKDKLIEQGHQVDQINVRNQLWKSTSF
                     KDYDVLIHTAALVHNNSPQARLSDYMQVNMLLTKQLAQKAKAEDVKQFIFMSTMAVYG
                     KEGHVGKSDQVDTQTPMNPTTNYGISKKFAEQALQELISDSFKVAIVRPPMIYGAHCP
                     GNFQRLMQLSKRLPIIPNINNQRSALYIKHLTAFIDQLISLEVTGVYHPQDSFYFDTS
                     SVMYEIRRQSHRKTVLINMPSMLNKYFNKLSVFRKLFGNLIYSNTLYENNNALEIIPG
                     KMSLVIADIMDETTTKDKA"
     gene            14114..15376
                     /gene="cap8O"
                     /locus_tag="O2701_001096"
     CDS             14114..15376
                     /gene="cap8O"
                     /locus_tag="O2701_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8O"
                     /protein_id="HCZ4293000.1"
                     /translation="MKLTVVGLGYIGLPTSIMFAKHGVDVLGVDINQQTIDKLQSGQI
                     SIEEPGLQEVYEEVLSSGKLKVSTTPDASDVFIIAVPTPNNDDQYRSCDISLVMRALD
                     SILSFLEKGNTIIVESTIAPKTMDDFVKPVIENLGFTIGEDIYLVHCPERVLPGKILE
                     ELVHNNRIIGGVTEACIEAGKRVYRTFVQGEMIETDARTAEMSKLMENTYRDVNIALA
                     NELTKICNNLNINVLDVIEMANKHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKL
                     IQTGREINNSMPAYVVDTTKQIIKVLSGNKVTVFGLTYKGDVDDIRESPAFDIYELLN
                     QEPDIEVCAYDPHVELDFVEHDMSHAVKDASLVLILSDHSEFKNLSDSHFDKMKHKVI
                     FDTKNVVKSSFEDVSYYNYGNIFNFIDK"
     gene            15453..16598
                     /gene="cap8P"
                     /locus_tag="O2701_001097"
     CDS             15453..16598
                     /gene="cap8P"
                     /locus_tag="O2701_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8P"
                     /protein_id="HCZ4293001.1"
                     /translation="MKKIMVIFGTRPEAIKMAPLVKEIDHNGNFEANIVITAQHRDML
                     DSVLSIFDIQADHDLNIMQDQQTLAGLTANALAKLDSIINEEQPDMILVHGDTTTTFV
                     GSLAAFYHQIPVGHVEAGLRTHQKYSPFPEELNRVMVSNIAELNFAPTVIAAKNLLFE
                     NKDKERIFITGNTVIDALSTTVQNDFVSTIINKHKGKKVVLLTAHRRENIGEPMHQIF
                     KAVRDLADEYKDVVFIYPMHRNPKVRAIAEKYLSGRNRIELIEPLDAIEFHNFTNQSY
                     LVLTDSGGIQEEAPTFGKPVLVLRNHTERPEGVEAGTSRVIGTDYDNIVRNVKQLIED
                     DEAYQRMSQANNPYGDGQASRRICEAIEYYFGLRTDKPDEFVPLRHK"
     gene            complement(16663..16989)
                     /gene="isdI"
                     /locus_tag="O2701_001098"
     CDS             complement(16663..16989)
                     /gene="isdI"
                     /locus_tag="O2701_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdI"
                     /protein_id="HCZ4293002.1"
                     /translation="MFMAENRLQLQKGSAEETIERFYNRQGIETIEGFQQMFVTKTLN
                     TEDTDEVKILTIWESEDSFNNWLNSDVFKEAHKNVRLKSDDDGQQSPILSNKVFKYDI
                     GYHYQK"
     gene            complement(16996..17379)
                     /locus_tag="O2701_001099"
     CDS             complement(16996..17379)
                     /locus_tag="O2701_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HCZ4293003.1"
                     /translation="MRLILIVIGLIFTALGIAGAVLPLLPTTPFLLVAVFCFARSSDR
                     FYNWLINQKIYKEYVENFYLHRGYTLQQKIKILISLYIVIGFSIYMVDVLAVRVGLII
                     MVIIQTAVLFTFVKTLPKSNHKIEE"
     gene            17806..19293
                     /locus_tag="O2701_001100"
     CDS             17806..19293
                     /locus_tag="O2701_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCZ4293004.1"
                     /translation="MAVNVRDYIAENYGLFINGEFVKGSSDETIEVTNPATGETLSHI
                     TRAKDKDVDHAVKVAQEAFESWSLTSKSERAQMLRDIGDKLMAQKDKIAMIETLNNGK
                     PIRETTAIDIPFAARHFHYFASVIETEEGTVNDIDKDTMSIVRHEPIGVVGAVVAWNF
                     PMLLAAWKIAPAIAAGNTIVIQPSSSTPLSLLEVAKIFQEVLPKGVVNILTGKGSESG
                     NAIFNHDGVDKLSFTGSTDVGYQVAEAAAKHLVPATLELGGKSANIILDDANLDLAVE
                     GIQLGILFNQGEVCSAGSRLLVHEKIYDQLVPRLQEAFSNIKVGNPQDEATQMGSQTG
                     KDQLDKIQSYIDAAKESDAQILAGGHRLTENGLDKGFFFEPTLIAVPDNHHKLAQEEI
                     FGPVLTVIKVKDDQEAIDIANDSEYGLAGGVFSQNITRALNIAKAVRTGRIWINTYNQ
                     VPEGAPFGGYKKSGIGRETYKGALSNYQQVKNIYIDTSNALKGLY"
     gene            19939..20898
                     /locus_tag="O2701_001101"
     CDS             19939..20898
                     /locus_tag="O2701_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCZ4293005.1"
                     /translation="MNGKKANTINRYKYFHHVNHQKIQQSSKKTLWASLIITLLFTVI
                     EFVGGLVSNSLALLSDSFHMLSDVLALGLSMLAIYFASKKPTARYTFGYLRFEILAAF
                     LNGLALIVISIWILYEAIVRIIYPQPIESGIMFMIASIGLLVNIILTVILVRSLKQED
                     NINIQSALWHFMGDLLNSIGVIVAVVLIYFTGWRIIDPIISIVISLIILRGGYKITRN
                     AWLILMESVPQHLDTDQIMADIKNIDGILDVHEFHLWSITTEHYSLSAHVVLDKKYEG
                     DDYQAIDQVSSLLKEKYGIAHSTLQIENLQLNPLDEPYFDKLT"
     gene            complement(20960..21163)
                     /locus_tag="O2701_001102"
     CDS             complement(20960..21163)
                     /locus_tag="O2701_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293006.1"
                     /translation="MDKESVVASLARNKKIAVETMAGQRYIIERILHTNDEKHIHILK
                     PKDVVLDVDSIKEIDENHLNDAT"
     gene            21342..21854
                     /locus_tag="O2701_001103"
     CDS             21342..21854
                     /locus_tag="O2701_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HCZ4293007.1"
                     /translation="MTLFLLEANNLDFASTKEELEAKAASLSTKTIPTLIEVQATENL
                     THGYFIVEANDEAEAKQFLTEADISIQLVKEVRLVGKDLDEVKNGDAHVDYLVTWNIP
                     EGITMDQYLARKKKNSVHYEEVPEVEFKRTYVCEDMSKCICLYNAPDEEAVRRARKAV
                     DTPIDGIEKL"
     gene            22196..22936
                     /locus_tag="O2701_001104"
     CDS             22196..22936
                     /locus_tag="O2701_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4293008.1"
                     /translation="MIKIQQLQHHFGSHKVIHNFNLDISKGEIVTFIGKSGCGKSTLL
                     NIIGGFIHPSSGRVIIDNEIKQQPSPDCLMLFQHHNLLPWKTINDNIRIGLQQKISDE
                     EINAQLKLVDLEDRGKHFPEQLSGGMKQRVALCRAHVHKPNVISMDEPLGALDAFTRY
                     KLQDQLVQLKHKTQSTIILVTHDIDEAIYLSDRIVLLGEGCNIISQYEITASHPRSRN
                     DSHLLKIRNEIMETFALNHHQVEPEYYL"
     gene            22950..23924
                     /locus_tag="O2701_001105"
     CDS             22950..23924
                     /locus_tag="O2701_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCZ4293009.1"
                     /translation="MKRLSIIVIIGIFIITGCDWQRTSKERSKNAQNQQVIKIGYLPI
                     THSANLMMTKKLLSQYNHPKYKLELVKFNNWPDLMDALNSGRIDGASTLIELAMKSKQ
                     KGSNIKAVALGHHEGNVIMGQKGMHLNEFNNNGDDYHFGIPHRYSTHYLLLEELRKQL
                     KIKPGHFSYHEMSPAEMPAALSEHRITGYSVAEPFGALGEKLGKGKTLKHGDDVIPDA
                     YCCVLVLRGELLDQHKDVAQAFVQDYKKSGFKMNDRKQSVDIMTHHFKQSRDVLTQSA
                     AWTSYGDLTIKPSGYQEITTLVKQHHLFNPPAYDDFVEPSLYKEASRS"
     gene            23921..24682
                     /locus_tag="O2701_001106"
     CDS             23921..24682
                     /locus_tag="O2701_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4293010.1"
                     /translation="MTRPTNNKFILPIITFIIFLGIWEMVIIIGHYQPVLLPGPALVG
                     KSIWSFIVTGEIFQHLAISLWRFVAGFVVALLVAIPLGFLLGRNRWLYNAIEPLFQLI
                     RPISPIAWAPFVVLWFGIGSLPAIAIIFIAAFFPIVFNTIKGVRDIEPQYLKIAANLN
                     LTGWSLYRNILFPGAFKQIMAGIHMAVGTSWIFLVSGEMIGAQSGLGFLIVDARNMLN
                     LEDVLAAIFFIGLFGFIIDRFISYIEQFILRRFGE"
     gene            24695..>25601
                     /locus_tag="O2701_001107"
     CDS             24695..>25601
                     /locus_tag="O2701_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HCZ4293011.1"
                     /translation="MTLETLIKEQLDPHLVEVDEGTYYPRTFIQQLFVDGYFGEAALR
                     KNAEVIEAVSQSCLTTGFCLWCQLAFSTYLENATQPHLNNDLQQQLLSGEILGATGLS
                     NPMKSFNDLEKLNLEHTYVDGQLVVSGRMPAVSNIQEDHYFGAISKHESSDEFVMFIL
                     RANQDGITLVEKTNFLGVNGSATYQITLNQVVVPQSQIITHDAKQFAATIRPQFIAYQ
                     IPIGLGSIKSSLELIDAFSNVQNGINQYLEYDVEAFKKRYRQLREEYYAILDDGNLTS
                     HLNELISLKKDIGYLLLDVNQASVVN"
BASE COUNT         9001 a         3644 c         4817 g         8139 t
ORIGIN      
        1 taatgaaaat ttttaaggga ggtaaataat ggaaagtaca ttagaattaa caaaaattaa
       61 agaagtatta caaaaaaact tgaagatttt aattatttta ccgctattat ttttaattat
      121 tagcgctatt gttacatttt tcgtcttatc acctaaatat caagctaata ctcaaatttt
      181 agtgaatcaa actaagggtg acaatcctca gtttatggcg caagaggttc aaagtaatat
      241 tcaacttgta aatacgtata aagaaattgt taaaagtcct agaattttag atgaggtgtc
      301 aaaggactta aatgataagt attcaccatc taaattgtcg agtatgttga caattacaaa
      361 ccaagaaaat acgcaactta tcaacatcca agttaaaagt ggtcataaac aagattcgga
      421 aaaaattgcg aatagcttcg ctaaagttac aagtaaacaa attccgaaga ttatgagtgt
      481 ggataacgta tcaattttat ctaaagcaga cggtacagca gttaaagtcg caccaaaaac
      541 tgtagtgaat ctaatcggtg cattcttttt aggattagtt gtcgcgctta tatatatctt
      601 cttcaaagta attttcgata agcgaattaa agatgaagaa gatgtagaga aagaattagg
      661 attgcctgta ttgggttcaa ttcaaaaatt taattaagga tggttgctac ttatgtcaaa
      721 aaaggaaaat acgacaacaa cactatttgt atatgaaaaa ccaaaatcaa caattagtga
      781 aaagtttcga ggtatacgtt caaacatcat gttttcaaaa gcaaatggtg aagtaaagcg
      841 cttattggtt acttctgaaa agcctggtgc aggtaaaagt acagttgtat cgaatgtagc
      901 gattacttat gcacaagcag gctataagac attagttatt gatggcgata tgcgtaagcc
      961 aacacaaaac tatattttta atgagcaaaa taataatgga ctatcaagct taatcattgg
     1021 tcgaacgact atgtcagaag caattacgtc gacagaaatt gaaaatttag atttgctaac
     1081 agctggccct gtacctccaa atccatctga gttaattggg tctgaaaggt tcaaagaatt
     1141 agttgatctg tttaataaac gttacgacat tattattgtc gatacaccgc cagttaatac
     1201 tgtgactgat gcacaactat atgcgcgtgc tattaaagat agtctgttag taattgatag
     1261 tgaaaaaaat gataaaaatg aagttaaaaa agcaaaagca cttatggaaa aagcaggcag
     1321 taacattcta ggtgtcattt tgaacaagac aaaggtcgat aaatcttcta gttattatca
     1381 ctattatgga gatgaataag tatgattgat attcataacc atatattgcc taatatcgat
     1441 gacggtccga caaatgaaac agagatgatg gatcttttaa aacaagcgac aacacaaggt
     1501 gttacagaaa tcattgtaac atcacatcac ttacatcctc gatataccac acctatagaa
     1561 aaagtgaaat catgtttaaa ccatattgaa agcttagagg aagtacaagc actaaatcta
     1621 aagttttatt atggtcagga aataagaatt accgatcaaa tccttaatga tattgatcga
     1681 aaagttatta acggtattaa tgattcacgc tatttactaa tagaatttcc atcaaatgaa
     1741 gttccacact atactgatca attatttttc gaattacaga gtaaaggctt tgtaccgatt
     1801 attgcacatc cagagcggaa taaagcaata agtcaaaacc ttgacatact atacgattta
     1861 attaacaaag gtgctttaag tcaagtgaca acggcgtcat tagcgggtat ttccggtaaa
     1921 aaaattagaa aattagcaat tcaaatgatt gaaaacaatc tgacacattt catcggttca
     1981 gatgcgcata acacagaaat cagaccgttc ttaatgaaag acttatttaa tgataagaaa
     2041 ttacgtgatt attatgaaga tatgaacgga tttattagta atgcgaagtt agttgttgat
     2101 gataaaaaaa ttcctaaacg aatgccacaa caagattata aacagaaaag atggtttggg
     2161 ttataaacag caaatgaggg gttttatggc acatttatct gtgaaattgc ggcttttaat
     2221 actagcatta atcgattcac tgatagtgac attttcagta ttcgtaagtt attacatttt
     2281 agaaccgtat ttcaaaacat attctgtcaa attattaata ttggcagcta tatcactatt
     2341 catatcgcat catatttcag catttatttt taatatgtat catcgagcgt gggaatatgc
     2401 cagtgtgagt gaattgattt taattgttaa agctgtgacg acatctatcg ttattacgat
     2461 ggtggtcgtg acaattgtta caggcaatag accgtttttt agattgtatt taattacttg
     2521 gatgatgcac ttgattttaa taggtggctc aaggttattt tggcgtattt atcggaaata
     2581 ccttggaggt aagtcattta ataagaagcc aactttagtt gttggtgctg gtcaagcagg
     2641 ttcaatgctg attagacaaa tgttgaaaag tgacgaaatg aaacttgaac cggtattagc
     2701 agtcgatgat gacgaacata aacgcaatat cacaattact gagggtgtaa aagtccaagg
     2761 taaaattgcg gatattccag aactagtgag gaaatataag attaaaaaaa tcatcattgc
     2821 aattccaact attggtcaag agcgtttgaa agaaattaat aatatttgcc atatggatgg
     2881 cgttgagtta ttgaaaatgc caaatataga agacgtcatg tctggtgagt tagaagtgaa
     2941 ccaacttaaa aaagttgaag tagaagattt actaggcaga gatcctgttg aattagatat
     3001 ggatatgata tcaaatgaat tgacgaataa aactatttta gttacgggtg caggtggttc
     3061 aataggatca gaaatttgta gacaagtttg taatttctat ccagaacgta ttattctact
     3121 tggccatggt gaaaacagta tttatttaat caatcgtgaa ttgcgaaatc gcttcggaaa
     3181 aaaatgttga tatcgttcct attatagcgg atgtgcaaaa tagagcgcgt atgtttgaaa
     3241 ttatggaaac gtataaacca tacgcagttt atcatgcagc agcacacaag cacgtgccgt
     3301 taatggaaga caaccctgaa gaagcagtac gtaataatat tttaggtacg aaaaatactg
     3361 ctgaagctgc taaaaatgca gaggtaaaga aattcgttat gatttctacg gataaagccg
     3421 ttaatccgcc taatgtcatg ggagcttcaa agcgaattgc agaaatgatt attcaaagtt
     3481 taaatgatga aacgcatcga acaaattttg ttgcagtgag atttggtaat gtacttggat
     3541 cgagaggatc tgtgattcca cttttcaaaa gtcaaattga agaaggtggg ccagttactg
     3601 tgacacatcc tgaaatgaca cgttacttta tgacaattcc tgaagcttct agactagttt
     3661 tgcaggcagg ggcattagca gaaggtggcg aagtatttgt gctagatatg ggagaaccag
     3721 tgaaaattgt agatttggca cgtaatttaa tttagctaag tggtaaaaaa gaagacgaca
     3781 tacgcattac ttatacaggg attagacccg gcgaaaaaat gtttgaagag cttatgaata
     3841 aagatgaggt tcatcctgaa caagtatttg aaaaaattta tcgtggcaaa gtacaacata
     3901 tgaaatgtaa tgaagttgaa gcgattattc aagacatcgt caatgacttt agtaaagaaa
     3961 aaattattaa ctatgccaat ggcaaaaagg gagataatta tgttcgatga caaaatttta
     4021 ttaattactg ggggcacagg atcattcggt aatgctgtta tgaaacagtt tttagattct
     4081 aatattaaag aaattcgtat tttttcacgc gatgagaaaa aacaagatga cattcgaaaa
     4141 aaatataata attcaaaatt aaagttctac attggtgatg tgcgtgatag tcaaagtgta
     4201 gaaacagcaa tgcgagatgt ttattacgta ttccatgcag cagctttaaa acaagtgccg
     4261 tcatgtgaat tctttccagt tgaggcagtg aagacaaata ttattggtac agaaaatgtc
     4321 ttacaaagtg ctattcatca aaatgttaaa aaagtcatat gtttatctac agataaggca
     4381 gcgtatccta ttaatgctat gggtatttca aaagcaatga tggaaaaagt attcgtagcc
     4441 aaatcaagaa atattcgtag tgaacaaacg cttatttgtg gtacaagata cggtaatgtg
     4501 atggcttcaa gaggatcagt aatacctttg tttatcgaca aaatcaaagc tggagaacct
     4561 ttaacgatta cagatcctga tatgacaaga tttttaatga gcttagaaga tgcggtagaa
     4621 ctagttgttc atgcatttaa gcatgcagag acaggagata ttatggttca aaaagcacca
     4681 agctcaacgg taggggatct tgcgaccgca ttattagaat tgtttgaagc tgataatgca
     4741 attgaaatca ttggtacgcg acatggagag aaaaaagcag aaacattgtt gacgagagaa
     4801 gaatacgcac aatgtgaaga tatgggtgat tattttagag tgccggcaga ctccagagat
     4861 ttaaattata gtaattatgt tgaaaccggt aacgaaaaga ttacgcaatc ttatgaatat
     4921 aactccgata atacacatat tttaacggtg gaagagataa aagaaaaact tttaacacta
     4981 gaatatgtta gaaacgaatt gaatgattat aaagcttcaa tgagatagga gagattgacg
     5041 ttgaatattg taattacagg agcaaaaggt tttgtaggaa aaaacttgaa agcagattta
     5101 acttcaacga cagatcatca tattttcgaa gtacatcgac aaactaaaga ggaagaatta
     5161 gagtcagcat tgttgaaagc agactttgtc gtgcatttag cgggtgttaa tcgacctgaa
     5221 catgacaaag aattcagctt aggaaacgtg agttatttag atcatgtact tgatatatta
     5281 actagaaata cgaaaaagcc agcgatatta ttatcgtctt caatacaagc aacacaagat
     5341 aatccttatg gtgagagtaa gttgcaaggg gaacagctat taagagagta tgccgaagag
     5401 tatggcaata cggtttatat ttatcgctgg ccaaatttat tcggcaagtg gtgtaagccg
     5461 aattataact cagtgatagc aacattttgt tacaaaattg cacgtaacga agagattcaa
     5521 gttaatgatc ggaatgttga actaacgcta aactacgtgg atgatatcgt cgctgaaata
     5581 aagcgtgcta ttgaaggaac tccaacgatt gaaaatggtg tacctacagt accaaacgta
     5641 tttaaagtga cattgggaga aattgtagat ttattataca agttcaaaca gtcacgtctc
     5701 gatcgaacat tgccgaaatt agataacttg tttgaaaaag atttgtatag tacgtattta
     5761 agctatctac ctagtacaga ctttagttat cccttactta tgaatgtgga tgataggggt
     5821 tcttttacag aatttataaa aacaccggat cgtggtcaag tttctgtaaa tatttctaaa
     5881 ccaggtatta ctaaaggtaa tcactggcat catactaaaa acgaaaaatt tctagtcgta
     5941 tcaggtaaag gggtaattcg ttttagacat gttaatgatg atgaaatcat tgaatattat
     6001 gtttctggcg acaaattaga agttgtagac ataccagtag gatacacaca taatattgaa
     6061 aatttaggcg acacagatat ggtaactatt atgtgggtga atgaaatgtt tgatccaaat
     6121 cagccagata cgtatttctt ggaggtatag cgcatggaaa aactgaaatt aatgacaata
     6181 gttggtacaa ggcctgaaat cattcgttta tcatcaacga ttaaagcatg tgatcaatat
     6241 tttaatcaga tattagtaca cactggtcaa aattatgatt atacattgaa tcaaattttc
     6301 tttgatgatt tggaattaag acaaccggac cactacttag aggcagttgg aagtaacctt
     6361 ggagaaacga tggggaatat tattgcgaag acatatgatg ttttattacg cgaacaacca
     6421 gatgcacttt taattcttgg tgatacaaat agttgtttag cagcagtatc tgctaaacga
     6481 ttaaagattc ctgtgttcca catggaagcg ggtaatagat gctttgatca gaatgtacct
     6541 gaagaaatca atcgtaaaat tgttgaccat gtcagtgatg tgaatctacc ttatacggaa
     6601 catagcagac gttatttatt agatgaaggc ctcaataaag cgaatatctt tgtgacagga
     6661 tcaccgatga cagaagtgat agaagcgcat cgagataaaa ttaatcacag tgacgtttta
     6721 aataaactag gattagaacc gcaacaatac attttagtat ctgcgcatag agaagagaat
     6781 atcgataatg aaaagaattt taaatcatta atgaatgcga taaatgatat tgccaaaaag
     6841 tataaaatgc ctgtgattta ttcaacgcat ccaagaagtt ggaagaaaat tgaagaaagt
     6901 aaatttgaat ttgatccatt agttaaacag ttaaagccat ttggtttctt tgattataat
     6961 gcattgcaaa aagatgcatt tgttgtgcta tcagatagtg gaacattgtc agaagagtcg
     7021 tctattttga agttccctgg tgtccttatt cgaacttcca cagaaagacc ggaagtacta
     7081 gataaaggta cggttattgt aggtggtatt acctataaca atctaatcca atccgttgaa
     7141 ctagcaagag agatgcaaaa caataacgaa ccgatgattg atgctattga ttataaagac
     7201 actaacgttt cgacaaaggt agttaaaatt attcaaagct ataaagatat tatcaatcga
     7261 aatacttgga ggaaatgacg atgaggatag cgattgaaaa gataattggt ttgctgaaaa
     7321 accagtcctc taaagaatcg aatgttaaga ttcatcgctt ggcgtatatt acaaactcaa
     7381 aatttgatgg caataactat atagatagat ggtgtaaaat caggaattct cacattggtg
     7441 aatacagtta tattggattt ggtagtgatt ttaataatgt agaagtagga agatattgtt
     7501 cgatatcttc ggatgtaaaa attgggttag gaaaacatcc tacacacttt tttagctcat
     7561 caccgatttt ttattctaat aataatccat ttaacataaa gcaaaagttt atagacttta
     7621 atgaccaacc aagccgtaca acaattaaaa atgatgtgtg gattggtgca aatgtaatta
     7681 ttatggatgg tttaacaata aatactggtg cagtcatagc agccggctca gttgttacta
     7741 aaaatgtagg agcatatgag gttgttggtg gtgttcctgc aaaagtgatt aagaagcgat
     7801 ttgacaataa aacaattgaa aaacttttgg aaagcaagtg gtgggagaaa acgcctgaca
     7861 aactaaaagg attttcggtt gaatatttaa ataaaaagga tacttaatga tatgagaatt
     7921 ttaaatattg tatcgagtaa tattgttcaa gacccaaggg tacttaaaca aatagaaaca
     7981 attaaaggcg ttacggatga ttataaaatt gttggaatga ataattcaca agctactaat
     8041 aagcgattgg aaaatttaga ttgtaattat cgtttgttag gtagcaaggt agatccaaaa
     8101 aatattcttt ctaaattaat taagcgtata agatttgcaa caggtgttat ccgagaaatt
     8161 aaagcttata aacctgacgt gattcatgca aatgatttcg acgtattatt aatggtctat
     8221 ttaagcaatt ataaaaaagc taatattgtt tatgatgcgc atgaaatata tgcgaaaaat
     8281 gcctttatta ataaagttcc acttatttca aagtttgtag aaagtataga aaaacacata
     8341 gtaaaacatc gtgttaatgc cttcgtaaca gtaagtcatg cagcaaaaga atattatcaa
     8401 tctaaaggat ataagaagga agcgaatgtt attacgaatg cacctatttt aaatgatagc
     8461 agagaattta aagaaatcga aaactttaaa gaaattgtat atcaaggtca aattgtaatg
     8521 gacagaggat atgaagagtt tattattgct tcatcagctt ttaaacaaaa tgctccttca
     8581 ttcataattc gagggtttgg tccgcatgaa gaagtgataa aagaactgat tagttataac
     8641 ccggaaaata ttaggttgga taaaccagtt gaagtaaaag aattggttga taagttagca
     8701 gaaagtaatg ttggtgttgt cttgacgaaa ccagtatcta ttaattttga atatacagta
     8761 tctaataaaa tttttgaatg tatacatgct ggtttaccag taattttatc tcctgtcaaa
     8821 gagcatattt atctcaatga aaaatataaa tttggcattg ttctaaagga agttacgccg
     8881 ttagaaattg aaaaggcggt tagaaaatta agagataatc acgatttgtt taatcattta
     8941 cgtcaaaatg caattaaggc gtctaaaatt ttgaattggc aaatagagag tgaacggtta
     9001 gtagaattat ataaatttta aagagaggta gactatgaaa ttttttgtac tttgtgcaat
     9061 tatcagcatg aacatattta tagtaatctc tacatttact aaagaagtat tagggttccc
     9121 tatagagccg gtgtattact caaccatggt tggtatagca ttaattacta cggtgtttgc
     9181 tatttataag ataattgtca cccaagaaat tccgcgaggg ttaatattat taattgctat
     9241 atgtttgctt tatctagctt tttattattt ttcaccagat aaggaagaga aactagctaa
     9301 aaataatatt ctattctttt taacatgggc agttccagcg gcaattagtg gtatttatat
     9361 taaatatata aacaaggcta cggtagaaag attttttaaa ttagtatttt tcatattttc
     9421 tgtttcattt atttttgtaa ttttaatacc aaaacttaca ggtgagatac ctagctatat
     9481 caattttgga cttatgaact atcaaaacgc ttcgtacctt tcagcattta ctgccggatt
     9541 aggcatttat ttcattatga aaggttcagt taaacataag tggatatatg ttctatttac
     9601 aataattgat atccctattg tgtttatacc aggagggcgt ggaggtgcta ttttattaat
     9661 tctttacggc ttatttgcat ttatacttat tacgtttaaa agaggaatac ctatcgcagt
     9721 aaaaagcatt atgtatattt ttgcattaag catatctagt gtattgattt actttctttt
     9781 tacaaaaggt tcgaatacta gaacattttc atatctacaa ggtggaacac ttaatttaga
     9841 aggtacttct ggaagaggac cgatttatga aaaaggtatt tactttattc aacaaagttc
     9901 gttattaggc tatgggccat ttaactatta taaactaatc ggaaatatac cacataacat
     9961 cattattgag ttgattctat catttggctt attagggttt tttatcataa tgatttgcat
    10021 tttgctacta gtttataaaa tgattaggaa ctatgatcca aacactatag atttactcgt
    10081 tatgtttata gcaatctatc caatcacatt attaatgttt agttcaaatt atttagttgt
    10141 aagtgaattt tggtttgtgt tgttctattt tattacaaaa ggacggcgtc atcatggcta
    10201 agaaagtttt tattatggat agcgtaaaga caataattgg tacgttgctt atagctttag
    10261 gattacaatt tttagcttat ccaattatta atcaacgagt aggtaatgaa gcgttcggtt
    10321 ctattttaac gatttataca ataataacaa tcacgagtgt tgtattaggc aatacgctta
    10381 acaatatacg attgattaat atgaatctat acaaatccaa tcattactac tggaaatttg
    10441 catcgatact tttaatctca attctgattg agagtatagc tttaattatt gtatttcttt
    10501 acttttttaa tttgaacatc atcgatatta tctttttaat tctacttaat attttaatgt
    10561 gtttaaggat ttatctgaat gtatttttta ggatgacttt aaaatataat cagattttgt
    10621 atattgctct tattcaattt ttaggtttgc tgataggact atttctatat tatttaaccc
    10681 aaaactggat tgtttgtttt attaccagtg aattgtttgc aacgatatat acattggtta
    10741 aattacgggg attaactata ggcgagtatc aaagtgaaga taataatgtg gtaaaagatt
    10801 atgtgatgct actgagtaca aatagcctta ataatttgaa tctctattta gatagattaa
    10861 tcttattacc aattataggt ggaacagctg taactatatc atttctttca acatttattg
    10921 ggaaaatgtt agctacattt ctatatccga ttaataatgt agtactttca tatatttctg
    10981 taaatgaaag tgacaatata aagaagcaat atttgaaaac taatctaatt gctatagctg
    11041 ccctatgttt agtcatgatt atatgttatc caattacaat aattattgtc tctttactgt
    11101 ataacattga ttcaagttta tattcgaagt ttattatttt aggtaatata ggtgttttat
    11161 tcaatgcagt gagtattatg atccaaactt taaatacaaa acacgcatca ataacattac
    11221 aagcgaatta tatgacgctt cacacgatta catttatatt cataactatt ttaatgacaa
    11281 ttgcgtttgg tctaaatgga ttcttttgga caacgctgtt cagcaacatt attaagtatg
    11341 tgattttaaa tattataggt ttaaagtcta aattcattaa taaaaaggac gtcgattaga
    11401 tgagtgaaaa aaagattttg attttatgtc agtattttta tccggaatat gtatcttctg
    11461 cgacgttacc aactcaattg gcggaagatt taattgcgaa tcacattaat gtcgatgtca
    11521 tgtgtggatg gccatatgaa tatagtaatc ataaacaggt ttctaaaacc gagatgcatc
    11581 gtggtattcg cattcgacgt ctcaagtatt cgaggtttaa taacaaaagt aaggttggaa
    11641 ggatcatcaa tttctttagt ttattttcaa aattcgtgat taatatacct aaaatgttga
    11701 aatatgatca gattcttgtt tactctaatc caccaatctt gccattaata ccagacgttt
    11761 tacacagact gcttaagaaa aaatattctt ttgtggtgta tgatatagca cctgataatg
    11821 cgattaagac aggtgcaact cgtccaggta gcatgattga taagctgatg cgttacatta
    11881 atagacatgt ctacaagaat gctgaaaatg tcattgtcct tggtacggaa atgaaaaact
    11941 acttactaaa tcatcaaatt tctaaaaatg ctgacaatat ccatgtgatt cctaactggt
    12001 atgacatgcg tcaattacaa gacaatcgta tctataatga cacatttaaa gcttaccgtg
    12061 agcaatacga caaaatttta ttgtatagcg gtaatatggg gcagttacag gatatggaga
    12121 cacttatctc atttttaaaa ttaaataagg atcagtctca aacgttaaca atactttgtg
    12181 gtcatggtaa gaaatttgca gatgtcaaaa cggcaataga agaccatcgt attgaaaatg
    12241 ttaaaatgtt tgagttttta acaggtacag actatgctga cgtattaaaa attgcggatg
    12301 tatgtattgc atcgctgatt aaagaaggcg tcggtttagg cgtgccgagc aagaattatg
    12361 gctatcttgc agctaagaaa gcgttggtac tcatcatgga taagcaatct gatatcgttc
    12421 aacatgttga acaatatgat gcgggtatcc aaattgataa tggcgatgca catgccattt
    12481 ataacttcat caacactcac tcgagtaagg aattgcacga gatgggtgag cgcgcacatc
    12541 aactgtttaa agataaatat acgagagaaa ttaatactat gaagtattac aatctgttga
    12601 agtgaggaga taattatgaa gcgattattc gatgtagtga gttcaatata tggtttagta
    12661 gttttaagtc cgattctgtt aattacagca ttactaatta aaatggaatc acctggacca
    12721 gccattttca aacaaaaaag accgacgatt aataatgaat tgtttaatat ttataagttt
    12781 agatcaatga aaatagacac acctaatgtt gcaactgatt taatggattc aacatcgtat
    12841 ataacaaaga cagggaaggt cattcgtaag acctctattg atgaattgcc acaattattg
    12901 aatgttttaa aaggagaaat gtcaattgta ggtcctagac cagcgcttta taatcaatac
    12961 gaattaatcg aaaaacgtac aaaagcgaac gtgcatacga ttagaccagg tgtgacagga
    13021 ctagctcaag tgatggggag agatgatatc actgatgatc aaaaagtagc gtatgatcat
    13081 tattacttaa cacatcaatc tatgatgctt gatatgtata tcatatataa aacaattaaa
    13141 aatatcgtta cttcagaagg tgtgcatcac taatgagaaa aaatatttta attacaggcg
    13201 tacatggata tatcggtaat gctttaaaag ataagcttat tgaacaagga catcaagtag
    13261 atcaaattaa tgttaggaat caattatgga agtcgacctc gttcaaagat tatgatgttt
    13321 taattcatac agcagctttg gttcacaaca attcacctca agcaaggcta tctgattata
    13381 tgcaagtgaa tatgttgctg acgaaacaat tggcacaaaa ggctaaagct gaagacgtta
    13441 aacaatttat ttttatgagt actatggcag tttatggaaa agaaggtcat gttggtaaat
    13501 cagatcaagt tgatacacaa acaccaatga accctacgac caactatggt atttccaaaa
    13561 agttcgctga acaagcatta caagaattga ttagtgattc gtttaaagta gcaattgtga
    13621 gaccaccaat gatttatggt gcacattgcc caggaaattt ccaacggtta atgcaattgt
    13681 caaagcgatt gccaatcatt cccaatatta acaatcagcg cagtgcatta tatattaaac
    13741 atctgacagc atttattgat caattaatat cattagaagt gacaggtgtg taccatcctc
    13801 aagatagttt ttactttgat acatcgtcag taatgtatga aatacgtcgc caatcacatc
    13861 gtaaaacggt attgatcaac atgccttcaa tgctaaataa gtattttaat aagttgtcgg
    13921 tctttagaaa attattcggc aatttaatat acagcaatac gttatatgaa aataataatg
    13981 cacttgaaat tattcctgga aaaatgtcac ttgttattgc ggacatcatg gatgaaacga
    14041 caaccaaaga taaggcataa gtcatctatt aaataaaatc aacatacaaa tcgttttatt
    14101 tggaggttat agtatgaagt taacagtagt tggcttaggt tatattggtt taccaacatc
    14161 aattatgttt gcaaaacatg gcgtcgatgt gcttggtgtt gatattaatc agcaaacgat
    14221 tgataagtta caaagtggtc aaattagtat tgaagaacct ggattacaag aggtttatga
    14281 agaggtactg tcatcgggaa aattgaaggt atctacaacg ccagatgcat ctgatgtttt
    14341 tatcattgcc gttccgacgc cgaataatga tgatcagtac cggtcatgtg acatttcgct
    14401 agttatgcgt gcattagata gtattttatc atttttagaa aaaggaaata ccattattgt
    14461 agagtcgaca attgcgccta aaacgatgga tgattttgta aaaccagtca ttgaaaattt
    14521 agggtttaca ataggtgaag atatttattt agtgcattgt ccagaacgtg tactgccagg
    14581 aaaaatttta gaagaattag ttcataacaa tcgtatcatt ggcggtgtga ctgaagcttg
    14641 tattgaagcg ggtaaacgtg tctatcgcac attcgttcag ggagaaatga ttgaaacaga
    14701 tgcacgtact gctgaaatga gtaagctaat ggaaaacaca tatagagacg tgaacattgc
    14761 tttagctaat gaattaacaa aaatttgcaa taacttaaat attaatgtat tagatgtgat
    14821 tgaaatggca aacaaacatc cgcgtgttaa catccatcag cctggtccag gtgtaggcgg
    14881 tcattgttta gctgttgatc cgtactttat tattgctaaa gaccctgaaa atgcaaagtt
    14941 aattcaaact ggacgtgaaa ttaataattc aatgccggcc tatgttgttg atacaacgaa
    15001 gcaaatcatc aaagtgttga gcgggaataa agtcacagta tttggtttaa cttataaagg
    15061 tgatgttgat gatataagag aatcaccagc atttgatatt tatgagctat taaatcaaga
    15121 accagacata gaagtatgtg cttatgatcc acatgttgaa ttagattttg tggaacatga
    15181 tatgtcacat gctgtcaaag acgcatcgct agtattgatt ttaagtgacc actcagaatt
    15241 taaaaattta tcggacagtc attttgataa aatgaagcat aaagtgattt ttgatacaaa
    15301 aaatgttgtg aaatcatcat ttgaagatgt atcgtattat aattatggca atatatttaa
    15361 ttttatcgac aaataaaatg tgtcaaacta gggcatacat gattaaggaa agataagctg
    15421 tcatgtgttt gaacttcaga gaggataatg ttatgaaaaa aattatggtt attttcggta
    15481 cgagacccga agcaataaaa atggcaccat tagtaaaaga aattgatcat aatgggaact
    15541 ttgaagcgaa cattgtgatt acagcacaac atagagatat gttagatagt gtgttaagta
    15601 tatttgatat tcaagctgat catgatttaa atattatgca agatcaacaa acattagcag
    15661 gccttacggc gaatgcactt gctaaacttg atagcatcat taatgaggaa caaccggata
    15721 tgattttagt acatggtgat actacaacga cttttgtagg aagtttggca gcattttatc
    15781 atcaaattcc ggtcggacat gtagaagctg gacttcgaac acatcagaaa tactcaccat
    15841 ttcctgaaga gttaaatcga gtcatggtaa gtaatattgc tgaattgaat tttgcgccaa
    15901 cagtaattgc agctaaaaat ttactttttg aaaacaaaga caaagagcgt atctttatta
    15961 ctggaaatac agttattgac gcattgtcaa caacagttca aaatgatttt gtttcaacga
    16021 ttattaataa acataaaggc aagaaagttg ttttactaac agcgcatcgt cgtgaaaata
    16081 ttggggaacc gatgcatcag atttttaaag cagtaagaga tttggcagat gaatataaag
    16141 atgttgtctt catttatcca atgcatcgta atccaaaggt aagagcgatt gccgaaaaat
    16201 atttatctgg gagaaatcgg attgaattaa ttgagccatt agatgcgatt gagttccata
    16261 attttacaaa tcaatcgtac ctcgtgctga cagattctgg tggtattcaa gaggaggctc
    16321 ctacatttgg aaaacctgtg ttggtattaa ggaatcatac agagcgtccc gaaggcgttg
    16381 aggcgggaac atcgagagta attggcacag attatgacaa tattgttcga aatgtgaaac
    16441 aattgattga ggatgatgaa gcgtatcaac gtatgagtca agcgaataat ccatatggtg
    16501 atggacaagc atcacgacgt atttgtgaag caatagaata ttattttgga ttgcgcacag
    16561 acaagccgga tgaattcgta cctttacgtc acaaataata aaaaacccct aatcatgaag
    16621 ttggtttaga caaccagcgg tgactagggg tttttaatat atttattttt gatagtggta
    16681 gccaatatca tatttgaata ctttatttga taatattgga ctttgctgtc catcgtcatc
    16741 actttttaaa cgtacatttt tatgagcttc tttaaataca tcggaattca accaattatt
    16801 aaagctatct tcagattccc aaatagttaa gattttaact tcgtctgtat cctcggtatt
    16861 taatgtttta gtgacaaaca tttgttggaa gccttcaata gtttcaatac cttgtctatt
    16921 gtaaaaacgt tcaatcgttt cttccgcact gcctttttgt aattgtaatc tattttctgc
    16981 cataaacatg ggcaatcact cctctatttt atgatttgat ttgggtaatg tttttacaaa
    17041 tgtaaagagt acagcggttt gtatgataac cattatgatt aatcctacac ggactgcaag
    17101 aacatccacc atataaattg aaaaacctat tacaatgtat aagctaatta aaattttaat
    17161 tttctgttgt agcgtgtagc ctcgatgtaa ataaaagttt tctacatatt ctttataaat
    17221 tttttgatta ataagccaat tgtaaaagcg atctgaactt cgagcaaaac aaaaaactgc
    17281 tacgagtaaa aaaggggtcg ttggcagtaa aggtaatacg gcacctgcaa taccaagcgc
    17341 tgtaaatatt aagccaatga cgattaaaat aagtcgcatt gaaaaaactc cattctagta
    17401 ctaatgcgca tgtaatattg ttttagtaat ataactcatg ctaaatataa tgtgtatgat
    17461 aagtgcaatg actcagtaaa atgaaacgat gttgaattat ccttgtcaca ttaacgcatt
    17521 ttaagcgcga ctttcataac aaccaaacta tttaatgaga attattctca agtattatag
    17581 ttatattatg tgttttattt ttgaaaagtg caatatgttt tcgaaaataa gattattttt
    17641 atgtgcaaaa acgacgcaaa agttttaaaa atgagacttc tgtgagctga ttattttata
    17701 aaatgtaaac gcttactata taatgtgaat catatcgttt aaaagcatta ttaaatatga
    17761 tgctaagaga tttatattat agccaataaa caaaggagag ataatatggc agtaaacgtt
    17821 cgagattata ttgcagagaa ttatggttta tttatcaatg gggaatttgt taaaggtagc
    17881 agtgacgaaa caatcgaagt gactaatcca gcaactggag aaacactatc acatattaca
    17941 agagcaaaag ataaagatgt cgatcatgca gtcaaagtgg cgcaagaggc atttgaatca
    18001 tggtcattaa cttctaaatc agaacgtgca caaatgttgc gtgatattgg tgataaatta
    18061 atggcacaaa aagataaaat tgcaatgatt gaaacattaa ataatggtaa accgattcgt
    18121 gagacaacag caattgatat tccatttgct gcaagacatt tccattattt cgcaagtgtt
    18181 attgaaacag aagaaggtac agtgaatgat atcgataaag acacaatgag tatcgtacga
    18241 catgagccga ttggcgtcgt aggtgctgtt gttgcttgga acttcccaat gctattagct
    18301 gcatggaaga ttgcgccagc cattgctgca ggtaatacaa ttgtgattca accttcgtct
    18361 tcaacaccat taagtttatt ggaagttgct aaaattttcc aagaggtatt acctaaaggt
    18421 gttgtcaata tactaacggg taaaggttca gaatcaggta atgcaatttt caatcatgat
    18481 ggtgtagata aattatcatt tacgggctca actgatgtag gttatcaagt tgccgaagct
    18541 gcagcaaaac atctagtacc cgctacatta gagcttggtg gtaaaagcgc caatatcata
    18601 ttagatgatg ctaatttaga ccttgcagtt gaaggtattc agttaggtat tttattcaac
    18661 caaggtgaag tatgtagtgc aggttctcga ttattagttc atgaaaaaat ttatgatcaa
    18721 ttggtgccac gtttacaaga ggcattttca aatattaaag ttggaaatcc acaagatgaa
    18781 gctacacaaa tgggtagtca aactggtaag gatcaattag ataaaattca atcatatatt
    18841 gatgcagcaa aagaatcaga tgcacaaatt ttagcaggcg gtcatcgctt aactgaaaat
    18901 ggattagata aagggttctt ctttgagccg acattaattg ctgtgccaga caatcatcac
    18961 aaattagcac aagaagaaat atttggacca gtgttaacag tgattaaagt gaaggacgat
    19021 caagaagcaa ttgatatagc taatgattct gagtatggtt tagcaggcgg tgtattttct
    19081 caaaatatca cacgtgcatt aaatattgct aaagctgtac gtacaggacg tatttggatt
    19141 aacacttaca accaagtacc agaaggcgca ccatttggtg gttataaaaa atcaggtatc
    19201 ggtcgagaaa cttataaagg tgcgttaagt aactatcaac aagttaaaaa tatttatatt
    19261 gatacaagca atgctttaaa aggtttgtac tagaataaat atcgtttctg aagcgtgttt
    19321 gtaggtcagt ctagcggtaa gtcttaacat ttaacggcgt tgtttagatt ttaagcaaaa
    19381 caaaatatat aggaacacgt atcatgatat taggatataa tgactaaaat aatagcagta
    19441 ggatggtttt taattgcaaa tcatcttact gctgttttta attatgctaa tttgcgatgc
    19501 ggctattata aggacagagt tgtttattaa ttatggtgat ttagaaatat gaagttcaat
    19561 atgcaaagtc atcgtttgtt ttaatatgcg gaacaatcat taaagttatt gcgatttttt
    19621 gaacttaatg aaactaaaca ataaatttga gatacttttt tgtcattttt atgtaactaa
    19681 cacaataatc tcgtacatta ttaaaatttt ctatatgata ggaataaagc aaagcgcgag
    19741 tgtgctgtaa aagttttcca aggtgatatt acataaagct ataaagggta aagattaatg
    19801 agttgtcatg taaatgacga tgatgtataa atcatggtta attacggaag cattaatatt
    19861 aacctgagaa gctataaaga attattttta aaagcgacaa tattaaatac gacgcattta
    19921 tttaggagtg gcaaacgtat gaatgggaaa aaggcgaata cgataaacag atacaaatat
    19981 tttcatcatg tcaatcatca aaaaattcaa caaagttcta aaaagacgct gtgggcatca
    20041 ctaatcatca cattgttatt tacagtgatt gaatttgtcg gaggtttagt atctaattca
    20101 ttggcattac tgtcagattc atttcatatg cttagtgatg tattagcact tggtttatct
    20161 atgttggcca tttattttgc aagtaaaaag ccgactgcac gatacacatt tggatattta
    20221 agatttgaga tattagctgc atttttaaat ggtttagcat taattgtaat ttcaatctgg
    20281 attttatatg aagctattgt acgtattatt tatccgcaac caattgaaag tggcattatg
    20341 tttatgattg ctagtattgg tttactcgtc aatattattt tgactgttat ccttgtaagg
    20401 tctttaaaac aagaagacaa tatcaatatt caaagtgcat tatggcattt catgggagac
    20461 ttattgaact ctattggtgt catcgttgca gttgtattga tttactttac aggatggcgc
    20521 atcatcgacc caatcattag tattgtaatt tcactcatca ttttacgtgg tggttataaa
    20581 attacgcgta atgcgtggtt aattttaatg gaaagtgtgc ctcaacattt ggatactgat
    20641 caaattatgg cagatattaa aaacatagat ggcatattag atgtacatga atttcatttg
    20701 tggagtatta caacagagca ttattcatta agtgcccatg ttgtgttaga taaaaaatat
    20761 gagggtgatg attatcaagc gattgatcaa gtatcatcat tgttgaaaga aaaatatggc
    20821 attgcacatt caacgttgca aattgaaaac ttgcaattga atccattaga tgagccatac
    20881 ttcgacaaat taacataaat aaaacattgt agcgcctaaa acattaatct atgtcatagg
    20941 cgcacgtttc gttttatact tatgttgcat catttaaatg attttcgtca atttctttga
    21001 tgctatctac atctaacacg acatctttag gtttcaaaat atgaatatgt ttttcatcat
    21061 ttgtatgtaa aatgcgttct atgatgtacc tttgaccggc cattgtttct acagcaatct
    21121 ttttgtttct agctaaactt gctacgacag attctttatc cataatgata gccccctata
    21181 tatatgttta tttacttata ccctaacatg atttttatac tctttgaaaa tatattttac
    21241 agaattttat ctaaatattt aaaaaaatat cttaatatcc ttgtaatccg ataagaatta
    21301 tagtaatatt ttttcaacca ttgttatagg aggtcttatt aatgacatta tttttattag
    21361 aagctaacaa tcttgatttt gcatcaacga aagaagaact agaagcaaag gcagcatcac
    21421 tatctacgaa gacaattcca acattaattg aagtacaagc tactgaaaat ttaactcatg
    21481 gttattttat tgtggaagca aatgacgaag cagaagctaa acaattttta acagaagcag
    21541 atattagtat tcaattagtc aaagaagtac gcttagttgg taaagattta gatgaagtta
    21601 aaaatggtga tgcacatgtt gattaccttg taacttggaa cattccggaa ggcattacga
    21661 tggatcaata tttagcacgt aaaaagaaaa attctgttca ttatgaagaa gtgccagaag
    21721 ttgaatttaa acgcacatat gtatgtgaag atatgtctaa atgtatttgt ttatacaacg
    21781 cacctgatga agaagcggta cgtcgcgcgc gcaaagcagt tgatacaccg attgatggca
    21841 tcgaaaaact ttaataagac aacaagttga tgagatatat gtatataggt ttggcatgga
    21901 tttcgattgc agttaattag aatagctcaa tgctataaat gtaagtagtt gatatgaaga
    21961 aactaatgaa ctaaatgcaa gtattgtcta aaacaatcat tttattgaaa tttagtagag
    22021 ctgaaattaa tataacgtcg ttaattgaat aacgcttatg ttataagagc actcatacca
    22081 aaccataatc atctatagat ataacaattc acgatataag ggctgtgttt ggcatagccc
    22141 tttagatata cacttaattc ctattaaaat agtaggggtt aaaagggggc ttgtcatgat
    22201 taaaattcaa caattacaac atcactttgg atcacataaa gtaattcata actttaattt
    22261 ggacattagc aagggagaaa tagtcacttt catagggaaa agtggttgcg gaaagtctac
    22321 tttactcaat attatcggtg gatttattca tccatcgtct ggtcgtgtca ttattgataa
    22381 cgaaattaaa caacagccat ctccagattg tttaatgcta tttcaacatc ataatttgct
    22441 gccatggaaa acgattaatg acaacattag gattggatta caacagaaaa ttagtgatga
    22501 agagattaac gcacagctta aattagttga tttagaagac aggggaaagc attttcccga
    22561 gcaactgtcc gggggtatga aacaacgtgt ggcactatgt cgagcgcatg tgcataagcc
    22621 taacgttata tcgatggatg agccattagg tgcattagat gcatttacac gttataaact
    22681 tcaggatcaa ctagtgcaac taaaacataa aacgcaatca actattattt tagtgacgca
    22741 tgacattgat gaagctattt atctttccga ccgcattgtt ctgttaggtg aagggtgcaa
    22801 tattatttct caatatgaaa ttacagcatc acatccacgc agtcgtaatg atagccacct
    22861 acttaagatt cgtaatgaaa ttatggaaac atttgcattg aatcatcatc aagttgaacc
    22921 tgaatattat ttataaggag tgagtgacga tgaaaaggtt aagcataatc gtcatcattg
    22981 gaatctttat aattacagga tgtgattggc aaaggacgtc taaagaacgg tctaaaaatg
    23041 cccaaaatca gcaagtgatt aaaattggat atttgccgat tacacattca gctaatttga
    23101 tgatgactaa aaaattatta tcacaataca atcatccgaa atataaacta gaattagtta
    23161 aattcaataa ttggccagat ttaatggacg cattaaacag tggtcgtatt gatggtgcat
    23221 caactttaat agagctagcg atgaaatcaa aacagaaggg ctcaaatata aaggctgtgg
    23281 cattgggcca tcatgaaggc aatgtcatta tgggacaaaa aggtatgcac ttaaatgaat
    23341 ttaataataa tggcgatgat taccattttg gtataccaca tcgttattca acacattatc
    23401 ttttacttga ggaattacgt aaacaattaa agattaaacc ggggcatttt agctatcatg
    23461 aaatgtcgcc agcagaaatg ccagccgcat tgagtgaaca cagaattaca gggtattctg
    23521 tagccgaacc attcggtgca ctgggtgaaa agttaggcaa aggtaagact ttgaaacatg
    23581 gtgatgacgt tatacctgat gcgtattgct gtgtgctagt actgagaggg gaattgcttg
    23641 atcaacacaa ggatgtagcg caagcatttg tacaagatta taaaaagtct ggctttaaaa
    23701 tgaatgatcg caagcaaagt gtagacatta tgacgcatca ttttaaacaa agtcgtgacg
    23761 ttttaacaca gtcagcggca tggacatcct atggtgattt aacaattaag ccatccggct
    23821 atcaagaaat tacgacattg gtaaaacaac atcatttgtt taatccacct gcatatgatg
    23881 actttgttga accgtcattg tataaggagg catcgcgttc atgacacgtc ccacaaataa
    23941 caaatttata ttacctatta tcacatttat tattttctta ggcatttggg aaatggtcat
    24001 tattattggg cattaccaac ctgtattgtt accgggtcct gctcttgtag gaaaaagtat
    24061 atggtctttc attgttactg gagaaatttt ccaacattta gcaattagtt tatggagatt
    24121 tgtagcgggc tttgttgtcg cattgttggt tgctattcca ttgggcttct tgcttggaag
    24181 gaatcgttgg ctatacaacg ctatcgaacc gctatttcaa ttgattaggc cgatatctcc
    24241 gatagcatgg gcaccatttg ttgttctatg gtttggtatt ggtagtttgc cagcgattgc
    24301 gattattttt atcgctgctt ttttcccaat tgtgttcaat actattaaag gcgttagaga
    24361 cattgaacct caatatttaa aaatagcagc aaatttaaat ttaactgggt ggtcattgta
    24421 tcgcaatata ttatttcccg gggcatttaa acaaatcatg gctgggatac atatggcggt
    24481 aggaacaagt tggatatttt tagtttctgg tgaaatgatt ggtgcacaat cgggattagg
    24541 ttttttaatc gttgatgcac gaaatatgtt gaacttagaa gatgttttag cagcaatatt
    24601 ctttatcgga ttatttggtt ttattattga tcgattcatt agttatattg agcagtttat
    24661 acttagaaga tttggtgaat aaggagagat gatgatgact ttagaaacgc ttatcaaaga
    24721 acaattagat cctcatttag tagaagttga tgaagggacg tattatccga gaacatttat
    24781 tcagcaatta tttgtagatg gttatttcgg tgaggcggca ttgagaaaaa atgctgaagt
    24841 aatcgaagct gtatcgcagt cttgtttgac aacaggattt tgtttatggt gccaattagc
    24901 tttttcaacg tatttagaaa atgccacgca gccacattta aataatgact tacaacagca
    24961 attgttatct ggagaaatat taggtgctac cggattgtct aatccgatga agtcatttaa
    25021 tgatttagaa aagttgaacc ttgaacacac ttatgttgat ggacaattgg ttgtcagtgg
    25081 acgtatgcca gctgtaagta atattcaaga agaccattat tttggtgcga tttcgaaaca
    25141 tgaatcatca gatgaatttg tcatgttcat tctacgtgcc aatcaagatg gtatcactct
    25201 tgttgaaaaa accaattttt taggagtcaa cgggtcagca acgtatcaaa tcacattgaa
    25261 tcaagtcgta gtgccacaat cacaaattat cacgcatgat gcgaagcagt ttgcggcaac
    25321 tattcgcccg caatttattg cttaccaaat tccaatagga ttaggctcaa ttaaaagttc
    25381 tttagagtta attgatgcat tttcaaatgt gcaaaacgga ataaatcaat atttagagta
    25441 tgatgttgaa gcttttaaaa aacgttatcg tcaacttaga gaggaatatt atgcaatatt
    25501 agatgacggt aacttaactt cacatttaaa tgaattaata tcattgaaga aggacatcgg
    25561 ctatttattg ttagatgtaa atcaagcttc tgttgtcaat g
//
LOCUS       DALAGD010000033        25412 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.033, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000033 DALAGD010000000
VERSION     DALAGD010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 25412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25412
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.033"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            896..1090
                     /locus_tag="O2701_001108"
     CDS             896..1090
                     /locus_tag="O2701_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCZ4293012.1"
                     /translation="MADESKFEQAKGNVKETVGNVTDNKNLENEGKEDKASGKAKEFV
                     ENAKEKATDFIDKVKGNKGE"
     gene            complement(1323..2174)
                     /locus_tag="O2701_001109"
     CDS             complement(1323..2174)
                     /locus_tag="O2701_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HCZ4293013.1"
                     /translation="MQQFKWINILKGFAMGTSDLVPGVSGGTIALLLGIYNQFIASIS
                     GIFSRRFWPSFTFLIPIIIGMLLAMGSLSNLFNYLLSQHHIPTMFFFGGLIIGIVPYL
                     LKISNYKTSFTTKHYMMVIAGIAILIVITLMNNGDKHAGETLTLSTSLIIKYFIAGMC
                     ASSAMLLPGISGSFMLLVFGVYGTVMLAISEVVKLNFAGLPILLAVGFGVLAGFIISS
                     KIIQYFLTHHKLMTFALIIGFVVGSLFAVFPGLPTNIVMWFVSLVVFIIGFIVSLTLG
                     RITAENE"
     gene            2502..3263
                     /gene="sufC"
                     /locus_tag="O2701_001110"
     CDS             2502..3263
                     /gene="sufC"
                     /locus_tag="O2701_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCZ4293014.1"
                     /translation="MASTLEIKDLHVSIEDKEILKGVNLTINTDEIHAIMGPNGTGKS
                     TLSSAIMGHPSYEVTKGEVLLDGVNILELEVDERAKAGLFLAMQYPSEITGVTNADFM
                     RSAINAKREEGQEINLMQFIKKLDKNMDFLDIDKDMAQRYLNEGFSGGEKKRNEILQL
                     MMLEPKFAILDEIDSGLDIDALKVVSKGINQMRGENFGALMITHYQRLLNYITPDKVH
                     VMYAGKVVKSGGPELAKRLEEEGYEWVKEEFGSAE"
     gene            3361..4668
                     /gene="sufD"
                     /locus_tag="O2701_001111"
     CDS             3361..4668
                     /gene="sufD"
                     /locus_tag="O2701_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCZ4293015.1"
                     /translation="MTTDILNISEEQLVDYSKAHNEPSWMTELRKKALKLTETLEMPK
                     PDKTKLRKWDFDSFKQHDVKGDVYQSLSQLPESVREIIDVDHSKNLVIQHNNTIAYTQ
                     VDDNASKDGVIVEGLADALMNHSDLVQKYFMKDAVTVDEHRITALHTALVNGGVFVYV
                     PKNVVVEHPVQYVVLHDDENASFYNHVIIVTEESAEVTYVENYLSNASGEGNQLNIIS
                     EVIAGANSNITYGSVDYMDKGFTGHIIRRGITEADASINWALGLMNEGSQIIDNTTNL
                     FGDRSTSSLKSVVVGTGEQKINLTSKIVQYGKETDGYILKHGVMKEHASSVFNGIGYI
                     KHGGTKSIANQESRVLMLSEHARGDANPILLIDEDDVQAGHAASVGRVDPDQLYYLMS
                     RGISQREAERLVIHGFLDPVVRELPIEDVKRQLREVIERKVSK"
     gene            4774..6024
                     /locus_tag="O2701_001112"
     CDS             4774..6024
                     /locus_tag="O2701_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCZ4293016.1"
                     /translation="MNEVAEHSFDVNEVIKDFPILDQKVNGKRLAYLDSTATSQTPVQ
                     VLNVLEDYYKRYNSNVHRGVHTLGSLATDGYENARETVRRFINAKYFEEIIFTRGTTA
                     SINLVAHSYGDANVEEGDEIVVTEMEHHANIVPWQQLAKRKNATLKFIPMTADGELNI
                     EDIKQTINDKTKIVAIAHISNVLGTINDVKTIAEIAHQHGAIISVDGAQAAPHMKLDM
                     QEMNADFYSFSGHKMLGPTGIGVLFGKRELLQKMEPIEFGGDMIDFVSKYDATWADLP
                     TKFEAGTPLIAQAIGLAEAIRYLERIGFDAIHKYEQELTIYAYEQMSAIEGIEIYGPP
                     KDRRAGVITFNLQDVHPHDVATAVDTEGVAVRAGHHCAQPLMKWLNVSSTARASFYIY
                     NTKEDVDQLINALKQTKEFFSYEF"
     gene            6014..6478
                     /locus_tag="O2701_001113"
     CDS             6014..6478
                     /locus_tag="O2701_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HCZ4293017.1"
                     /translation="MNFNNLDQLYRSVIMDHYKNPRNKGVLDNGSMTVDMNNPTCGDR
                     IRLTFDIEDGIIKDAKFEGEGCSISMASASMMTQAVKGHSLGEAMQMSQEFTKMMLGE
                     DYVITEEMGDIEALQGVSQFPARIKCATLAWKALEKGTVAKEGKAEGTTEEE"
     gene            6629..8026
                     /gene="sufB"
                     /locus_tag="O2701_001114"
     CDS             6629..8026
                     /gene="sufB"
                     /locus_tag="O2701_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCZ4293018.1"
                     /translation="MAKKAPDVGDYKYGFHDDDVSIFRSERGLTENIVREISNMKNEP
                     EWMLDFRLKSLKLFYKMPMPQWGGDLSELNFDDITYYVKPSEQAERSWDEVPEEIKRT
                     FDKLGIPEAEQKYLAGVSAQYESEVVYHNMEKELEEKGIIFKDTDSALQENEELFKKY
                     FASVVPAADNKFAALNSAVWSGGSFIYVPKNIKLDTPLQAYFRINSENMGQFERTLII
                     ADEGASVHYVEGCTAPVYTTSSLHSAVVEIIVHKDAHVRYTTIQNWANNVYNLVTKRT
                     FVYENGNMEWVDGNLGSKLTMKYPNCVLLGEGAKGSTLSIAFAGKGQVQDAGAKMIHK
                     APNTSSTIVSKSISKNGGKVIYRGIVHFGRKAKGARSNIECDTLILDNESTSDTIPYN
                     EVFNDQISLEHEAKVSKVSEEQLFYLMSRGISEEEATEMIVMGFIEPFTKELPMEYAV
                     EMNRLIKFEMEGSIG"
     gene            8213..8350
                     /locus_tag="O2701_001115"
     CDS             8213..8350
                     /locus_tag="O2701_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HCZ4293019.1"
                     /translation="MMVDKATTQIQHDCGIRVLVFIKLIESYIKYSLDNSILVRLRFV
                     V"
     gene            complement(8356..8670)
                     /locus_tag="O2701_001116"
     CDS             complement(8356..8670)
                     /locus_tag="O2701_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293020.1"
                     /translation="MYFVLAIFTIISASVSLGYSIQACASSHNINAYYALSRSLPLFL
                     LAIFSLVIHSAIFLITISIAMILVQFLDAIVGYKSEDVFKTYGPLATSVVNLILLIVF
                     LF"
     gene            9035..10075
                     /locus_tag="O2701_001117"
     CDS             9035..10075
                     /locus_tag="O2701_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCZ4293021.1"
                     /translation="MIIAIIILIFISFFFSGSETALTAANKTKFKTEADKGDKKAKGI
                     VKLLEKPSEFITTILIGNNVANILLPTLVTIMALRWGISVGIASAVLTVVIILISEVI
                     PKSVAATFPDKITRLVYPIINICVIVFRPITLLLNKLTDSINRSLSKGQPQEHQFSKE
                     EFKTMLAIAGHEGALNEIETSRLEGVINFENLKVKDVDTTPRINVTAFASNATYEEVY
                     ETVMNKPYTRYPVYEGDIDNIIGVFHSKYLLAWSNKKENQITNYSAKPLFVNEHNKAE
                     WVLRKMTISRKHLAIVLDEFGGTEAIVSHEDLIEELLGMEIEDEMDKKEKEKLSQQQI
                     QFQQRKNRNVSI"
     gene            10089..11156
                     /locus_tag="O2701_001118"
     CDS             10089..11156
                     /locus_tag="O2701_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HCZ4293022.1"
                     /translation="MWNKNRLTQMLSIEYPIIQAGMAGSTTPKLVASVSNSGGLGTIG
                     AGYFNTQQLEDEIDYVRQLTSNSFGVNVFVPSQQSYTSSQIENMNAWLKPYRRALHLE
                     EPVVKITEEQQFKCHIDTIIKKQVPVCCFTFGIPSEQIISRLKAANVKLIGTATSVDE
                     AIANEKAGMDAIVAQGSEAGGHRGSFLKPKNQLPMVGTISLVPQIVDVVSIPVIAAGG
                     IMDGRGVLASIVLGAEGVQMGTAFLTSQDSNASELLRDAIINSKETDTVITKAFSGKL
                     ARGINNRFIEEMSQYEGDIPDYPIQNELTSSIRKAAANIGDKELIHMWSGQSPRLATT
                     HPANTIMSNIINQINQIMQYK"
     gene            11223..11321
                     /locus_tag="O2701_001119"
     CDS             11223..11321
                     /locus_tag="O2701_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293023.1"
                     /translation="MRFYFTNLIKRKHQAEDRRKILIKQKHQTKVR"
     gene            11369..12217
                     /locus_tag="O2701_001120"
     CDS             11369..12217
                     /locus_tag="O2701_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCZ4293024.1"
                     /translation="MINIGLTGWGDHYSLYEDLERQTDKLKTYAGHFPVVELDATYYA
                     IQPERNILKWIKETPDTFEFVVKIHQALTLHADYKTFADTRQELFDQFKNMLEPLHTQ
                     KKLAMVLVQFPPWFDCNAQNIKYILYVRQQLQAFPMCVEFRHQSWFSDAFKEQTLAFL
                     TEHQIIHAVVDEPQVKDGSVPLVNRITNEIAFVRYHGRNHYGWTKKDMSDQEWRDVRY
                     LYDYNEQELIDLAQKAQILAQKAKKVYVIFNNNSGGHAANNAKTYQRLLNIEYEGLAP
                     QQLKLF"
     gene            12230..13057
                     /locus_tag="O2701_001121"
     CDS             12230..13057
                     /locus_tag="O2701_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCZ4293025.1"
                     /translation="MLLTITLLVLIGGLSAIIGSIVGIGGGIIIVPTMVYLGVEHGLL
                     HNITTQVAIGTSSVILIVTGLSSSLGYLKTKQVDIKNGSIFLFGLLPGSLLGSFISRY
                     LTFESFNLYFGIFLIFVAILLMVRNKIKPFKIFDKPKYEKTYVDAKGKTYHYSVPPLF
                     AFITTFLIGILTGLFGIGGGALMTPLMLIVFRFPPHVAVGTSMMMIFFSSVMSSIGHI
                     AQGHVAWGYAIILIISSYFGAKIGVKVNQSIKSDTVVTLLRTVMLLMGIYLIIRALI"
     gene            13084..14403
                     /locus_tag="O2701_001122"
     CDS             13084..14403
                     /locus_tag="O2701_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCZ4293026.1"
                     /translation="MRLTIYHTNDIHSHLHEYERIKAYMAEHRPRLNHPSLYVDLGDH
                     VDLSAPITEATLGKKNVALLNEAKCDVATIGNNEGMTISHEALNHLYDEAKFIVTCSN
                     VIDESGHLPNNIVSSYIKDIDGVKILFVAATAPFTPFYRALNWIVTDPLESIKEEIEL
                     QRGKFDVLIVLSHCGIFFDETLCQELPEIDVIFGSHTHHYFEHGEINNGVLMAAAGKY
                     GNYLGEVNLTFEAHKVVHKTAKIIPLETLPEVETSFEEEGKTLMSNSVIQHPVVLKRS
                     MNHITEAAYLLAQSVCEYTHAQCAIINAGLLVKDIVKDEVTEYDIHQMLPHPINMVRV
                     RLFGVKLKEIIAKSNKQEYMYEHAQGLGFRGNIFGGYILYNLGYIHSTGRYYLNGEEI
                     EDDKEYVLGTIDMYTFGRYFPTLKELPKEYLMPEFLRDIFKEKLLEY"
     gene            14487..15404
                     /gene="lipA"
                     /locus_tag="O2701_001123"
     CDS             14487..15404
                     /gene="lipA"
                     /locus_tag="O2701_001123"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCZ4293027.1"
                     /translation="MATKNEEILRKPDWLKIKLNTNENYTGLKKMMREKNLNTVCEEA
                     KCPNIHECWGARRTATFMILGAVCTRACRFCAVKTGLPNELDLNEPERVAESVELMNL
                     KHVVITAVARDDLRDAGSNVYAETVRKVRERNPFTTIEILPSDMGGDYDALETLMASR
                     PDILNHNIETVRRLTPRVRARATYDRTLEFLRRSKELQPDIPTKSSIMVGLGETIEEI
                     YETMDDLRANDVDILTIGQYLQPSRKHLKVQKYYTPLEFGKLRKVAMDKGFKHCQAGP
                     LVRSSYHADEQVNEAAKEKQRQGEAQLNS"
     gene            15534..15917
                     /locus_tag="O2701_001124"
     CDS             15534..15917
                     /locus_tag="O2701_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HCZ4293028.1"
                     /translation="MIKVDQHYFELIENYRECFNEEQFIARYSDILDKYDYIVGDYGY
                     DQLRLKGFYKDSNKKAEMSKRFSNIQDYIFEYCNFGCPYFVLRHLSKQEVKKLIEEVH
                     PSDVIDDDNKLQDVKIKPTIQDTEH"
     gene            complement(15996..16262)
                     /locus_tag="O2701_001125"
     CDS             complement(15996..16262)
                     /locus_tag="O2701_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3055 domain-containing protein"
                     /protein_id="HCZ4293029.1"
                     /translation="MIDMYLYDDNEESQVQFVGFVGEHSRYDLMLVHTNRHYGKTLVL
                     NMQTNKFGIIGTDDLKEEGYIAHILGVNAEEGDEITEYLNEVIH"
     gene            16360..16794
                     /locus_tag="O2701_001126"
     CDS             16360..16794
                     /locus_tag="O2701_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HCZ4293030.1"
                     /translation="MYFVDKDKLTQKLAYLQALTDDYHESKHNHYAFERIAQMLIESS
                     VDIGNMIIDAFILRDPGNYKDVIDILELENVITKETQQAINKTVGIRKQFTYDYTALD
                     IKIIMPMFDDALPYYKQFITEVTTFLHQENVPVTAFGKGENQ"
     gene            16794..17573
                     /locus_tag="O2701_001127"
     CDS             16794..17573
                     /locus_tag="O2701_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HCZ4293031.1"
                     /translation="MKQYKAYLIDLDGTMYMGTDEIDGAKQFIDYLNVKGIPHLYVTN
                     NSTKTPEQVTEKLREMHIDAKPEEVVTSALATADYISEQSPGASVYMLGGSGLNTALT
                     EAGLVIKNDEHVDYVVIGLDEQVTYEKLAIATLGVRNGATFISTNPDVSIPKERGLLP
                     GNGAITSVVSVSTGVSPQFIGKPEPIIMVKALEILGLDKSEVAMVGDLYDTDIMSGIN
                     VGMDTIHVQTGVSTLEDVQNKNVPPTYSFKDLNEAIAELEK"
     gene            17606..18565
                     /locus_tag="O2701_001128"
     CDS             17606..18565
                     /locus_tag="O2701_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HCZ4293032.1"
                     /translation="MVKIVVSRKIPDKFYQQLSKLGDVVMWQKSLVPMPKDQFVTALR
                     DADACFITLSEQIDAEILAQSPNLKVIANMAVGYDNIDVESATANNVVVTNTPNVLTE
                     TTAELGFTLMLAIARRIVEAEKYVEADAWQSWGPYLLSGKDVFNSTIGIYGMGDIGKA
                     FARRLQGFNTNILYHNRSRHKDAEADFNATYVSFETLLAESDFIICTAPLTKETHHKF
                     NAEAFEQMKNDAIFINIGRGQIVDETALIDALDNKEILACGLDVLANEPIDHTHPLMG
                     RDNVLITPHIGSASVTTRDNMIQLCINNIEAVMTNQVPHTPVN"
     gene            18819..18929
                     /locus_tag="O2701_001129"
     CDS             18819..18929
                     /locus_tag="O2701_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293033.1"
                     /translation="MMTTNYYVESIKLKLNFIMNIDIMNCKKQILKRILY"
     gene            18977..19129
                     /locus_tag="O2701_001130"
     CDS             18977..19129
                     /locus_tag="O2701_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HCZ4293034.1"
                     /translation="MKSKSKQPPNKYVEAFKPYLLTLLYLAIFITLYLIYGSGDTHNN
                     FIYNEF"
     gene            19145..20602
                     /gene="dltA"
                     /locus_tag="O2701_001131"
     CDS             19145..20602
                     /gene="dltA"
                     /locus_tag="O2701_001131"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HCZ4293035.1"
                     /translation="MTDIINKLQAFADANPQSIAVRHTTDELTYQQLMDESSKLAHRL
                     QGSKKPMILFGHMSPYMIVGMIGAIKAGCGYVPVDTSIPEDRIKMIINKVQPEFVFNT
                     TDESFESLEGEVFTIEDIKTSQDPVIFDSQIKDNDTVYTIFTSGSTGEPKGVQIEYAS
                     LVQFTEWMLELNKSGNEQQWLNQAPFSFDLSVMAIYPCLASGGTLNLVDKNMINKPKL
                     LNEMLTATPINIWVSTPSFMEMCLLLPTLNEEQYGSLNEFFFCGEILPHRAAKALVNR
                     FPSATIYNTYGPTEATVAVTSIQITQEILDQYPTLPVGVERPGARLSTTDEGELVIEG
                     QSVSLGYLKNDQKTAEVFNFDDGIRTYHTGDKAKFENGQWFIQGRIDFQIKLNGYRME
                     LEEIETQLRQSEFVKEAIVVPVYKNDKVIHLIGAIVPTTEVTDNAEMTKNIKNDLKSR
                     LPEYMIPRKFEWMEQLPLTSNGKIDRKKIAEVING"
     gene            20599..21813
                     /gene="dltB"
                     /locus_tag="O2701_001132"
     CDS             20599..21813
                     /gene="dltB"
                     /locus_tag="O2701_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PG:teichoic acid D-alanyltransferase DltB"
                     /protein_id="HCZ4293036.1"
                     /translation="MIPYGDFTFFLIALIALLPVIILGFLGKRSYIYNGVVTAFMIVL
                     IFSSDKHNLFDQKYLSVQLISFIIYVVWQVLLIMFYYHSKPKNNSFSKFVTVMVLSIL
                     PLALVKVLQSTWLGGHQIHFHESKLIEFVGFLGISYVTFKSVQLIMEIRDGSIKEIKV
                     WKLIQFISFFPTISSGPIDRYKRFVKDDKKVPTGNEYRELVLKAIHMIMLGFLYKYIV
                     AYFINTYAIMPLQLDLHGFVNLWLYMYAYSLYLFFDFAGYSLFAIAFSYLFGIKTPPN
                     FDKPFKAKNIKDFWNRWHMTLSFWFRDCIYMRSLFYMSRKKLLKSQFAMSNVAFLINF
                     FIMGIWHGIEVYYIVYGLYHAALFIGYGYYERWRKKHPPRWQNGFTTALSIVITFHFV
                     TFGFLIFSGKLI"
     gene            21831..22067
                     /gene="dltC"
                     /locus_tag="O2701_001133"
     CDS             21831..22067
                     /gene="dltC"
                     /locus_tag="O2701_001133"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     2"
                     /protein_id="HCZ4293037.1"
                     /translation="MEFREQVLNLLAEVAENDIVKENPDVEIFEEGIIDSFQTVGLLL
                     EIQNKLDIEVSIMDFDRDEWATPNKIVEALEELR"
     gene            22064..23239
                     /gene="dltD"
                     /locus_tag="O2701_001134"
     CDS             22064..23239
                     /gene="dltD"
                     /locus_tag="O2701_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HCZ4293038.1"
                     /translation="MKLKPFLPILISGAVFIVFLLLPASWFTGLVNEKTVEDNRTSLT
                     DQVLKGTLIQDKLYESNKYYPIYGSSELGKDDPFNPAIALNKHNANKKAFLLGAGGST
                     DLINAVELASQYDKLKGKKLTFIISPQWFTNHGLTNQNFDARMSQTQINQMFQQKNMS
                     TELKRRYAQRLLQFPHVHNKEYLKSYAKNPKETKDSYISGFKENQLIKIEAIKSLFAM
                     DKSPLEHVKPATKPDASWDEMKQKAVEIGKADTTSNKFGIRDQYWKLIQESKRKVRRD
                     YEFNVNSPEFQDLELLVKTMRAAGADVQYVSIPSNGVWYDHIGIDKERRQAVYKKIHS
                     TVVDNGGKIYDMTDKEYEKYVISDAVHIGWKGWVYMDEQIAKHMKGEPQPEVDKPKN"
     gene            complement(23502..23744)
                     /locus_tag="O2701_001135"
     CDS             complement(23502..23744)
                     /locus_tag="O2701_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HCZ4293039.1"
                     /translation="MPTEDTTMFDQVAEVIERLRPFLLRDGGDCSLIDVEDGIVKLQL
                     HGACGTCPSSTITLKAGIERALHEEVPGVIEVEQVF"
     gene            23845..24168
                     /locus_tag="O2701_001136"
     CDS             23845..24168
                     /locus_tag="O2701_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzD family protein"
                     /protein_id="HCZ4293040.1"
                     /translation="MEHVSVVVYGADVICASCVNAPTSKDIYDWLQPLLKRKYPNISF
                     KYTYIDITKDNDNLTDHDLQFIERIEQDELFYPLITMNDEYVADGYIQTKQITRFIDQ
                     KLVNE"
     gene            complement(24227..25291)
                     /locus_tag="O2701_001137"
     CDS             complement(24227..25291)
                     /locus_tag="O2701_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCZ4293041.1"
                     /translation="MKNLVLLGGGYGNMRIMSRILTTSLPQDYTVTLVDRMPFHGLKP
                     EFYALAAGTKSDKDVRMKFPNHPQVNTVYGEINDIDLDAQIVSVGNSKIDYDELIIGL
                     GCEDKYHNVPGAEEYTHSIQTLSKARDTFHSISELPEGAKVGIVGAGLSGIELASELR
                     ESRSDLEIYLYDRGPRILRNFPEKLSKYVAKWFAKNNVTVVPNSNINKVEPGKIYNCD
                     EPKDIDLVVWTAGIQPVEVVRNLPIDINSNGRVIVNQYHQVPTYRNVYVVGDCADLPH
                     APSAQLAEVQGDQIADVLKKQWLNEPLPDKMPELKVQGIVGSLGDKQGFAYIMDRTVT
                     GRLASILKSGVLWLYKYHNG"
BASE COUNT         9119 a         3714 c         4609 g         7970 t
ORIGIN      
        1 tcgttattta tgctataatc attttagaca cagcaatgtg ttcaaatttt catctattcg
       61 taagttagcc ttcgggctga ctttttattt ccattattca catgttaatc ttgttgttat
      121 ttaggcaggt acttcggtac ttgtctattt ttttatgtta taattacatg tgtatatagt
      181 aggagtgaac tatatagccc ggcagaggcc atatatctgg ctgttggtct cacaggagac
      241 atcttccttg tcatcactca tatacatata tcttgataac atagagttgt tatagtcgct
      301 acgccactca tactagttac tgggtggttg tttttatgtt atattataaa tgatcaaacc
      361 acaccaccta ttaatttagg agtgtggttg ttttcaaggg caaaaaaagg gcataatttt
      421 gaaacaaagg gcaagggtat gaataataaa ttatcttgaa aattttataa atgcatatat
      481 aacaatgttt tgattgccct tgaacttcct tgataatctg gctaattatt cctgctaaat
      541 aaatcatatt aatggctcag taacgtgatg ttgctgggct ttttaattta aacaggtatt
      601 tatatgatat ttaggaatga gatgctatta cggataaagt aaaatcctat atgcatgcgt
      661 gctactcatt tattcttatt tttaatttca catggctagt gactaataca tcataaatca
      721 ttagtgattc tttattattt cagtcccact ccctcaagtt aatgatgaat ctatttcgct
      781 agttataaaa tgacgtcaaa tcaatatcaa aaattattta agtaaaatgt ttagataatt
      841 tttcagtggg taagtattat atataacatt taattattcc gaggaggcat ttattatggc
      901 agacgaaagt aaatttgaac aagcaaaagg taatgttaaa gaaacagtag gtaatgttac
      961 tgataataaa aatttagaaa acgaaggtaa agaagataaa gcttctggta aagcgaaaga
     1021 attcgttgaa aatgcaaaag aaaaagcaac tgattttatt gataaagtaa aaggtaacaa
     1081 aggcgagtaa ttttactttt tattcctaat acactgcaca tgccacttag tcggtataac
     1141 catcggctag gtggtttttg tattaaaaaa gtggatacca aaatttatta ataattattt
     1201 taatgttaga aaaaaactaa ataaaaactc gctaatgata tccaataata tgtatacaaa
     1261 acgagacata tattgcatat gattaacgag atactgaaaa tattttatca cccctaaaat
     1321 gattattcat tttcagcggt aattcgacct aaagtcaaac ttacaataaa accgatgata
     1381 aatactacta atgaaacgaa ccacatcacg atattagttg gtaaacctgg aaatactgca
     1441 aagagggagc caacaacaaa accaatgatt aatgcaaaag tcattagttt atgatgtgtt
     1501 aggaaatact ggataatttt gcttgaaata atgaatccag caagcacgcc aaatccgact
     1561 gcaagtaata taggaagacc tgcaaagtta agtttaacaa cttcagatat tgctagcatg
     1621 accgtaccat agacgccaaa tactaataac ataaatgacc ctgaaatacc tgggagtaac
     1681 atagcactag atgcacacat acctgcaata aaatatttaa taataagact agttgataga
     1741 gtaagtgttt ctccagcatg tttatcacca ttattcatta atgtaataac aattaagata
     1801 gcgataccag ctataaccat catgtaatgt ttagttgtaa atgacgtttt atagttagaa
     1861 attttcaata aatatggaac gataccaatg attaatccac caaagaaaaa catagttgga
     1921 atatggtgtt ggcttaataa ataattaaaa agattactta gtgatcccat tgccagtaac
     1981 attccaatta taatggggat taaaaatgta aaacttggcc aaaaacgtcg tgagaatatg
     2041 ccgctaattg aagcgataaa ttgattgtaa atacctaaca ataatgcgat agtcccaccg
     2101 ctaacaccag gtaccaagtc actcgttccc atagcaaaac cttttagaat attaatccat
     2161 ttaaattgtt gcatgaataa ctcctttcaa acgattggaa taaaatcata aatagcatca
     2221 taccatatta caaatgtcct agtgaaatga taacatattt taaattcata aaatccattg
     2281 agaaattatg tgcacttatt atcatttata tttttaaaga gagcggatta gagttgtact
     2341 ttagagttat taaaaataat aaaaagggtt taagttgttt atattaaggt ataagtaagt
     2401 tataattaac tgaacgcatt attacaaagt ctttttgact acaaattaaa attattataa
     2461 actagttaag aaaactttat attttacgga gggaatataa aatggcatca acattagaaa
     2521 tcaaagacct acatgtgtct attgaggata aagaaatctt aaaaggtgtt aacttgacaa
     2581 ttaacactga tgaaatacat gcgattatgg gaccaaacgg gacaggtaaa tcaactttat
     2641 catctgcaat tatgggacac ccaagctatg aagtaactaa aggagaagta cttttagacg
     2701 gtgtaaatat tttagaatta gaagttgatg aaagagcaaa agcaggatta ttcttggcaa
     2761 tgcaatatcc atcagaaatt acaggtgtta caaatgctga tttcatgcgt tcagcaatca
     2821 atgcgaaacg tgaagaagga caagaaatca acttaatgca atttattaag aaattagata
     2881 aaaacatgga ttttctagac atagataaag acatggcaca acgttattta aatgaaggtt
     2941 tctcaggtgg agagaagaaa cgtaacgaaa tcttacaatt aatgatgtta gaacctaagt
     3001 ttgcaatctt agatgaaatc gattcagggt tagacatcga tgcattaaaa gttgtatcta
     3061 aaggtattaa ccaaatgcgt ggggaaaact ttggtgcatt aatgattaca cactatcaac
     3121 gattattaaa ttacattact cctgataaag tacatgtaat gtatgctggt aaagtcgtta
     3181 aatctggtgg tccagaatta gcaaaacgtc ttgaagaaga aggatatgaa tgggttaaag
     3241 aagagttcgg ttcagctgaa taatcttatt aatacagtat ccatgagatg ttcatctata
     3301 tatgatgaaa atgaacattt atacgaaata gtaaatttca tcaagtagga ggaaaaagtt
     3361 atgacaactg atattttgaa catttctgaa gaacaacttg ttgattattc taaagcccac
     3421 aatgaacctt cttggatgac agaattacgt aaaaaagctt tgaaattaac agaaacttta
     3481 gaaatgccaa aacctgataa aacaaaatta agaaaatggg attttgattc ttttaaacaa
     3541 cacgatgtaa aaggtgatgt ttatcaatct ttatcacaat tacctgagtc agtaagagaa
     3601 attattgacg tagatcattc taaaaactta gtaattcaac ataataatac gattgcgtac
     3661 acacaagttg atgataatgc atcgaaagat ggcgttatcg ttgaaggttt agcagacgct
     3721 cttatgaacc atagtgattt agtacaaaag tactttatga aagatgcagt aacagtagat
     3781 gaacatcgta tcacagcgct acacacggca ttagttaatg gtggcgtatt tgtttatgtt
     3841 cctaaaaatg tagttgtaga acatccagta caatacgttg tgttgcacga cgacgaaaat
     3901 gcaagctttt ataaccatgt tatcatcgtt actgaagaaa gcgccgaagt cacatatgtt
     3961 gaaaattact tatcaaatgc atctggtgaa ggaaatcaat taaatattat ttctgaagtg
     4021 attgctggtg caaattcaaa tatcacatat ggctcagtgg actatatgga taaaggcttt
     4081 acaggtcata tcattcgacg tggtattact gaagcggatg cctcaattaa ttgggcacta
     4141 ggtttaatga atgagggtag ccaaattatt gataatacaa caaatttatt tggtgatcgt
     4201 tcaacaagtt cacttaaatc agtagttgta ggtacaggcg aacaaaaaat taatctaaca
     4261 tctaaaatcg tacaatatgg taaagaaaca gatggttata tccttaaaca tggtgttatg
     4321 aaagaacatg catcgtctgt atttaatggt atcggctaca ttaagcatgg tggaactaaa
     4381 tcaattgcta atcaggaatc acgtgtatta atgttatctg aacatgctcg tggtgacgcg
     4441 aatcctattt tattaattga tgaagatgat gtacaagctg gtcatgctgc atcagtaggt
     4501 cgtgttgatc cagatcaact ttactattta atgagtcgtg gtatttctca aagagaagcg
     4561 gaacgtcttg ttatacatgg tttcttagat ccagtagtac gtgaattacc tatcgaagac
     4621 gttaaacgtc aattgagaga agtaattgaa cgcaaagttt ctaaataata ttttgaaaat
     4681 aaaagtttgt aatagatata gactgtcgat attggtataa gactaataca acgtcagtat
     4741 ttaaatgatt aggattttta tttaagaaag gtcgtgaatg aagtggccga acactcattt
     4801 gacgttaatg aagtaatcaa ggattttccg atattagatc aaaaagtcaa tggcaaacgt
     4861 ttagcatatc ttgattcaac agcgacaagt caaacgcctg tgcaagtgtt aaatgtttta
     4921 gaagattact acaagcgtta taattcaaac gttcatcgtg gtgttcatac attaggatca
     4981 ttggcaactg atggttatga aaatgcccgt gaaaccgttc gtcgttttat taatgcgaag
     5041 tattttgaag aaatcatttt tacacgcgga acaactgcgt cgattaacct tgtagcacat
     5101 agctatggtg atgcaaatgt tgaagagggc gatgaaattg ttgtcactga aatggaacat
     5161 catgccaata ttgttccttg gcaacagtta gcaaagcgta aaaatgcgac attgaaattt
     5221 ataccaatga cagctgacgg tgaattaaac atcgaggata ttaagcaaac gattaatgat
     5281 aaaacaaaga tcgttgctat tgcacatata tctaatgtgc tcggtacaat taatgatgtt
     5341 aaaaccattg cagaaatagc tcatcaacat ggtgcaatta tcagtgttga tggggcgcaa
     5401 gcagcaccac atatgaaact tgatatgcaa gaaatgaatg ctgattttta tagttttagt
     5461 ggtcataaaa tgcttggacc aacaggtatt ggcgtattat ttggtaaacg tgagttacta
     5521 caaaaaatgg aaccgattga gttcggtggc gacatgattg attttgtaag taagtatgat
     5581 gcaacatggg ctgatttacc tactaaattt gaggcgggta ctccattaat tgctcaagca
     5641 attgggcttg cagaagctat tcgctattta gaacgcatag gttttgatgc aattcataaa
     5701 tatgaacaag aattaacgat atatgcttat gagcaaatgt ctgcaattga aggaattgaa
     5761 atttatggcc cgccaaagga tcgtcgtgca ggtgtaataa cgtttaattt acaagatgta
     5821 catccacacg atgttgctac agccgtagat acagaaggtg tagcggttag agctgggcat
     5881 cattgtgcgc aaccgttaat gaaatggtta aatgtgtctt caacagctag agcgagtttt
     5941 tatatataca acacgaaaga agacgttgat cagttaataa atgccttgaa acaaacgaag
     6001 gagtttttct cttatgaatt ttaataatct agatcaatta tatagatctg tcattatgga
     6061 tcattataaa aatcctagaa ataaaggtgt attagataac gggtctatga cagtagatat
     6121 gaataacccg acatgcggtg accgtatacg actaacattt gatatagaag acggcattat
     6181 aaaagatgct aagtttgaag gtgaaggttg ttcgatttca atggcaagtg catcgatgat
     6241 gacacaagct gttaaaggtc attcacttgg agaagcaatg caaatgagcc aagaatttac
     6301 gaaaatgatg cttggtgaag actatgtgat tacagaagaa atgggagata ttgaagcatt
     6361 gcaaggtgta tctcaattcc cagctcgtat taaatgtgcc acattagctt ggaaagcatt
     6421 ggaaaaaggt actgttgcta aagaaggtaa agcagaaggt acgactgaag aagaatagaa
     6481 tgctgttaat catagataat tttgatatta gacatataaa agtataaaaa tttttataag
     6541 atgtcatgtc attgttataa tatggtttac atcatgaatt aaaaacttac gcacgccgtt
     6601 gtaaatatat ttttaaggag tgattgaaat ggctaaaaaa gcacctgatg ttggggatta
     6661 taaatatgga ttccacgacg atgatgtgtc cattttcaga tcagaacgtg gtttaactga
     6721 gaatatcgtt agagaaattt ctaacatgaa aaatgagccg gaatggatgt tagatttccg
     6781 tcttaaatca ttaaaattgt tttataaaat gccaatgcct caatggggtg gcgacttatc
     6841 agaattgaat ttcgatgaca ttacttacta tgtaaagcct tcagaacaag ctgaacgttc
     6901 atgggatgaa gtgccagaag aaattaaaag aactttcgat aaattaggaa ttcctgaagc
     6961 tgaacaaaaa tatttagctg gtgtttctgc tcaatatgaa tctgaagttg tttaccataa
     7021 tatggaaaaa gaacttgaag aaaaaggtat tatctttaaa gatacagata gtgctttaca
     7081 agaaaatgaa gaattattca aaaaatactt tgcttctgta gtacctgcag cagataacaa
     7141 atttgcggcg ttaaactcag cagtatggtc aggtggttcg ttcatttatg tacctaaaaa
     7201 tatcaaacta gatacgccac tacaagctta tttccgtatt aactctgaga acatgggtca
     7261 atttgaacgt acattaatca ttgctgatga aggtgcttct gtacattacg tagaaggttg
     7321 tactgcacca gtttatacaa ctagttcttt acactctgct gttgtggaaa tcattgtgca
     7381 taaagatgcg cacgttcgtt atactacgat tcaaaactgg gcgaacaatg tatacaattt
     7441 agttacaaaa cgtacttttg tttatgaaaa cggaaatatg gaatgggtag atggtaactt
     7501 aggttctaag ttaacgatga aatatccaaa ctgtgttctt ttaggtgaag gtgcaaaagg
     7561 tagtacatta tctattgcat ttgctggtaa aggacaagtt caagatgccg gtgctaaaat
     7621 gattcataaa gcacctaata catcttctac aattgtttct aaatctattt ctaaaaatgg
     7681 tggtaaagtt atttatcgcg gtattgttca ttttggacgt aaagcaaaag gtgctcgttc
     7741 aaatattgaa tgtgatacat taatcttaga taacgaatca acatcagata caattccata
     7801 taacgaagta ttcaacgatc aaatatcatt agaacatgaa gccaaggttt caaaagtttc
     7861 tgaagaacaa ttattctatc taatgagtcg tggtatttct gaagaagaag cgacagaaat
     7921 gattgttatg ggattcatcg aaccatttac aaaagaactt ccaatggaat acgcggtcga
     7981 aatgaaccgt ttaatcaagt tcgaaatgga aggtagtatt ggatagcttt aaaccgcgtt
     8041 gttaagccat tcttgacttc cgaaaatggc tattgatacc attttgatac tgaatataac
     8101 aaaaagccac attactgtgg ctttttttgt tttataacta aatcggattg atagataagc
     8161 tttgtactta tttatatcag ttcgattttt tgattggtgt aaaaaataat cattgatggt
     8221 ggataaagcg acaacacaaa tacaacatga ttgtggcatt agagtgctgg tctttattaa
     8281 attaattgaa agctacatca aatattcttt agataattcg atattagttc gattaagatt
     8341 cgttgtataa gtgagttaaa ataagaaaac tattaataat attaagttca ctacagatgt
     8401 tgctaatgga ccataagttt taaagacatc ttcactttta taaccaacaa tcgcatctaa
     8461 aaattgaact aagatcattg caatggatat agttatcaaa aatatagcac tatgaatgac
     8521 taaagaaaaa atagctaata aaaataaagg taagcttcga ctaagtgcat aatatgcatt
     8581 tatattatgg ctagatgcac atgcttgaat tgaataacct aaacttacac tggcactgat
     8641 tattgtaaat attgctaaaa caaaatacat gttaatcctt ctttctatat ttggatataa
     8701 acaagtactt gtctaaagtt atttaaaaga taattagaat aaatttatga gaaactggtt
     8761 gttatcatta taatggtttc aaatgattat aactatgtca taaactgaat ttgttgaaat
     8821 ttttcattat gcaaatttat taataacaaa cagctcgaac tatagcatca ttttactaat
     8881 gaatgcatta aagtaactat gactaaaaat gcatattaat tatcattatt aagactatta
     8941 tatataatga attttaactg gtttattaaa cgagaacgtc gggaattaag taactacaat
     9001 aaaaataaga tatgacaata aggagactac acgcgtgatc attgccataa ttatattgat
     9061 atttatttcg tttttctttt caggaagcga gacggcatta acggctgcca ataaaacaaa
     9121 atttaaaact gaagctgaca aaggtgataa aaaagcaaaa ggcattgtaa agttacttga
     9181 aaaaccaagt gagtttatta caacgattct aattgggaat aatgtcgcga atattttatt
     9241 accaacactt gttacaatta tggctttacg ttgggggatt agcgttggta ttgcatcagc
     9301 tgttttaaca gttgttatca ttttgatctc cgaagtgatt cccaagtctg tcgctgcaac
     9361 atttccagat aaaataacaa ggcttgtata tccaattatt aatatttgtg tcattgtgtt
     9421 ccgtcctatc acattacttt taaataagtt gacggacagt attaatcgaa gtttatctaa
     9481 gggccaacct caagaacatc aattttcaaa agaagaattt aaaacaatgt tagcaattgc
     9541 tggacatgaa ggtgctttaa atgaaattga gacgagtagg ttggaaggtg tcattaattt
     9601 tgaaaattta aaagtaaaag atgtagatac aacacctaga attaatgtga cggcatttgc
     9661 ttcaaatgcg acatacgaag aagtttatga aacggttatg aataagccat acactagata
     9721 tccagtgtac gagggagata ttgataacat tattggggtg tttcattcta aatatctgtt
     9781 ggcttggagt aataaaaaag aaaatcaaat tacaaactat tcagctaagc cattatttgt
     9841 gaatgaacac aataaagctg aatgggtatt acgtaagatg actatttcta gaaaacattt
     9901 agcaattgtg ttggacgaat ttggtggtac tgaagcgata gtgtcacatg aagacttaat
     9961 tgaagaatta ttaggtatgg aaattgaaga tgagatggat aaaaaggaaa aagaaaaact
    10021 ttctcaacag caaattcaat ttcaacaacg gaaaaatcgc aacgtatcta tataaggagc
    10081 gaacagctat gtggaataag aatcgactta ctcaaatgtt aagtattgaa tatccaatta
    10141 tacaagcagg tatggcagga agtacgacac cgaaattagt tgcatcagta agtaacagtg
    10201 gtgggttagg cacaataggc gcaggttact ttaatacgca gcaattggaa gatgaaatag
    10261 attatgtacg ccaattaacg tcaaattctt ttggcgtaaa tgtctttgta ccaagtcaac
    10321 aatcatatac cagtagtcaa attgaaaata tgaatgcatg gttaaaacct tatcgacgcg
    10381 cattacattt agaagagccg gttgtaaaaa ttaccgaaga acaacaattt aagtgtcata
    10441 ttgatacgat aattaaaaag caagtgcctg tatgttgttt tacttttgga attccaagcg
    10501 aacagattat aagcaggttg aaagcagcga atgtcaaact tataggtaca gcaacaagtg
    10561 ttgatgaagc tattgcgaat gaaaaagcgg gtatggatgc tatcgttgct caaggtagtg
    10621 aagcaggtgg acatcgtggt tcatttttaa aacctaaaaa tcaattacct atggttggaa
    10681 caatatcttt agtgccacaa attgtagatg tcgtttcaat tccggtcatt gccgctggtg
    10741 gaattatgga tggtagagga gttttggcaa gtattgtctt aggtgcagaa ggggtacaaa
    10801 tgggcaccgc atttttaaca tcacaagaca gtaatgcatc agaactactg cgagatgcaa
    10861 ttataaatag taaagaaaca gatacagtca ttacaaaagc gtttagtgga aagcttgcac
    10921 gcggtatcaa caataggttt atcgaagaaa tgtcccaata cgaaggcgat atcccagatt
    10981 atccaataca aaatgagcta acaagtagca taagaaaagc cgcagcaaac atcggcgaca
    11041 aagagttaat acatatgtgg agtggacaaa gcccgcgact agcaacaacg catcccgcca
    11101 acaccatcat gtccaatata atcaatcaaa ttaatcaaat catgcaatat aaataatcga
    11161 ccgcaatcca caaaagcaca agcaccccca aacattattt tagtgcttgc catttttgtg
    11221 gattgcgttt ctattttaca aatttaatca aacgaaaaca tcaagctgaa gatcgccgaa
    11281 agattttaat caagcaaaaa catcaaacta aagttcgctg aaatgattat gataaaagtt
    11341 atatggtatg atgacattgg tgatatatat gataaacatc ggattaacag gttggggtga
    11401 tcactattca ttatatgaag atttagaacg ccaaaccgat aaacttaaaa catatgctgg
    11461 acattttccg gttgtcgaat tagatgcgac atactatgcg atacaaccgg aaagaaatat
    11521 attgaaatgg ataaaagaaa cgcctgatac atttgaattt gtggtcaaaa ttcatcaagc
    11581 actcacattg catgcagact acaaaacatt tgcagataca aggcaagaac tatttgatca
    11641 atttaagaat atgttagagc ccttacatac acagaaaaaa ttagcaatgg tattggttca
    11701 atttccgcca tggtttgact gcaatgcaca aaatatcaaa tatattttgt atgtaagaca
    11761 gcaattacaa gcatttccaa tgtgtgtaga atttaggcat caatcatggt ttagtgatgc
    11821 atttaaagaa caaacattgg catttttaac agaacatcaa atcattcatg cagtagttga
    11881 tgaaccacaa gtgaaagatg gcagtgtacc tttagtcaat cgaatcacaa atgaaattgc
    11941 gtttgtacgt tatcatggac gtaatcatta cggttggact aagaaagata tgtcagatca
    12001 agaatggcgc gatgtacgct atttatatga ttataatgag caagaattaa tagacttggc
    12061 acaaaaggca caaatattag cacaaaaagc taagaaagtt tacgtcatat ttaacaataa
    12121 ttctggtggt catgcagcaa ataatgccaa aacatatcag cgattattga atatagaata
    12181 tgaagggtta gcaccacaac aattaaaatt attttaagag gcgacgacta tgttattaac
    12241 aattacatta ttagttttaa tcggaggttt gtcagcgatt atagggtcta tcgtaggcat
    12301 tggaggcggt attattatcg ttccaacaat ggtttacctc ggtgttgaac atggattact
    12361 acataatatt acaacacaag tagcgatagg gacgtcttca gtcattctaa ttgtgacagg
    12421 actttcttca tcacttggat atttaaaaac aaaacaagtt gatattaaaa atggttccat
    12481 ctttttattt ggactattac caggttcatt gcttgggtcc ttcattagta gatatttaac
    12541 atttgagtca tttaatttat attttggtat ctttttaatt ttcgtagcca ttttattaat
    12601 ggtaagaaat aagattaaac cgtttaaaat tttcgataaa cccaagtatg aaaagactta
    12661 tgtagacgct aaaggtaaaa catatcatta tagtgttcca ccattgtttg cttttattac
    12721 aacgttttta attggtatat tgacaggttt atttggtatt ggaggtggcg cactaatgac
    12781 gccactaatg cttattgtat ttagatttcc acctcatgta gctgttggaa caagtatgat
    12841 gatgattttc ttttcaagtg tcatgagttc tatagggcac attgctcaag gtcacgtagc
    12901 ttggggttat gcaatcattt taattatttc tagttatttt ggtgcgaaaa tcggtgtcaa
    12961 agtgaatcaa tcaattaagt cagatacggt agtaacatta ttgagaacag taatgttgtt
    13021 aatgggtata tatttaatta ttcgtgcgtt gatttaatac aactttaaaa ggaggacgtc
    13081 aatttgaggc ttacaattta tcatacgaac gatattcata gtcatttaca tgaatacgaa
    13141 cgcattaaag catatatggc agaacatcgg ccacgactta atcatccttc tttatatgtt
    13201 gatctaggtg atcatgtaga tttatccgca cctataactg aagcaacttt aggtaaaaag
    13261 aatgtggcat tactaaatga agcaaaatgt gatgttgcaa caatcggtaa taatgaaggg
    13321 atgaccattt cacacgaagc tttaaatcac ctttacgacg aagcaaaatt tatagtgaca
    13381 tgtagcaatg ttatagatga atcaggtcat ttaccaaata atatcgtttc ttcttatatt
    13441 aaggacatag acggtgtgaa aatactattc gttgcagcga cagcaccttt taccccattt
    13501 tatcgtgcac taaattggat tgttaccgat ccacttgaat ctataaaaga agaaattgaa
    13561 cttcaacgag gtaaatttga tgtattaatc gtgctaagtc attgtggcat tttcttcgat
    13621 gaaacattat gccaagaatt gcctgaaatt gatgtcattt ttggtagtca tacgcatcat
    13681 tattttgaac atggtgaaat caataatggt gtactgatgg cggcagctgg aaagtatggt
    13741 aattatcttg gagaggttaa tttaactttt gaggcacata aagtagtaca taaaactgca
    13801 aagattattc ctttagaaac attacctgaa gttgaaactt catttgaaga agaaggaaaa
    13861 acgttaatgt ccaattcagt aattcaacat ccagtagtgc ttaagcgtag tatgaatcac
    13921 ataactgaag ctgcatactt attagctcaa agtgtttgtg agtatacaca tgcacaatgt
    13981 gccatcatca atgctggctt actcgttaaa gatattgtaa aagatgaagt gacagaatat
    14041 gacattcatc aaatgttacc gcatccgatt aatatggtaa gggttagact ttttggtgtg
    14101 aaattaaaag agattatagc taaaagtaat aaacaagaat atatgtatga acatgcacaa
    14161 ggtttgggtt tcagagggaa tatatttgga ggatatattc tttataattt agggtacatt
    14221 cattctacag ggcgttacta tctgaatgga gaagaaatcg aagacgacaa agaatatgta
    14281 ctaggtacga tagatatgta tacgttcggt cgttatttcc caacattgaa agaattacca
    14341 aaagagtatt taatgccaga gtttttaaga gatatattta aagaaaaatt attggaatat
    14401 taaaaagtaa gattattgga ttttcatttg tcatgaattt cgatataatg tttaaagata
    14461 cacttaacag gagggtatgt gttgttatgg cgacaaaaaa cgaggaaata ttacgtaaac
    14521 cggattggtt gaaaataaaa ttaaatacca acgaaaacta tacaggactt aagaagatga
    14581 tgagggaaaa aaatcttaat actgtatgtg aagaagctaa atgtcctaat atacatgaat
    14641 gttggggtgc acgtcgtaca gcgacattta tgattttagg tgccgtatgt acaagagctt
    14701 gtcgtttttg tgcggttaag acaggtttac ctaatgaact tgatttaaat gagcctgaac
    14761 gtgtagctga atcagttgaa ttaatgaatt tgaaacacgt tgttatcact gctgttgcgc
    14821 gtgatgattt aagagatgct ggttcaaatg tttatgctga gacagtacgt aaagttagag
    14881 aaagaaatcc atttacaacg attgaaattt taccatcaga tatgggcggg gactatgatg
    14941 cgttagaaac attaatggcg tcaagacctg acattttaaa ccataatatt gaaactgttc
    15001 gtcgcttaac accgagagtt cgtgcgcgtg cgacttacga cagaacatta gagtttttac
    15061 gtcgttcaaa agaattacaa ccggatatcc caactaaatc aagtattatg gttggattag
    15121 gtgaaactat agaagaaatt tatgaaacga tggatgattt acgtgcgaat gatgtagata
    15181 ttttaacgat tggtcaatat ttacaacctt cacgtaaaca tttaaaggtt caaaaatatt
    15241 acacgccttt agagtttggt aaattaagaa aagtggcaat ggataaaggg tttaaacatt
    15301 gccaagctgg acctttagta cgtagttctt atcatgcgga tgagcaagta aatgaagctg
    15361 ctaaagaaaa gcaacgccaa ggtgaggcac agttaaatag ttaatattta accattaata
    15421 aggcataaag gcttagtttg tacaaaacga acgtgtcata gaagtaatca ttatttttat
    15481 gaaacactaa aagtataaac tgagcctttc attgtcatag ataggtgaag aatttgataa
    15541 aagtagatca acattacttt gaattaatag aaaattatcg cgaatgtttt aatgaagaac
    15601 aatttattgc taggtattca gatattttag ataaatatga ttacatagtt ggtgactatg
    15661 gttacgatca attacgatta aaaggttttt acaaagattc taataaaaaa gcagagatga
    15721 gtaaacgttt ttcaaatatt caagattaca tatttgaata ttgtaacttt ggttgtcctt
    15781 actttgtatt aagacatttg tctaaacaag aggttaaaaa gttaatcgaa gaagttcatc
    15841 cgtctgatgt gatagatgac gacaataaac ttcaagatgt gaagattaag ccaaccattc
    15901 aagatactga acattaataa aacccttagc tagattgaaa atgggaatca tgcaattcaa
    15961 gcatggacct gtaatctagt taggggtttt tatctttaat gaatgacttc atttaaatac
    16021 tcagtaattt catcgccttc ttcagcattt acacctaaaa tatgagcgat atagccttct
    16081 tcttttaaat catcagtacc gataataccg aatttatttg tttgcatatt aagtacgagt
    16141 gtcttaccat aatgtctatt tgtatggact aacatcaaat catatcgact atgctcgcca
    16201 acaaaaccaa caaactgaac ttgactctct tcgttgtcat catataaata catatcaatc
    16261 attttgtagc gactcctttt aaaagtagta aagttagtat aacgacaaat gaagtatact
    16321 gcaaaattat gataatatat aagtgagagg tgacaaggaa tgtattttgt agacaaagat
    16381 aaactaactc agaaattagc ctatttacaa gcattaactg atgattatca tgagagcaag
    16441 cacaatcatt atgcatttga acgcattgct caaatgttga tagaatcatc ggtagatata
    16501 gggaatatga ttatcgatgc atttatttta agggatcctg gtaattataa agatgtgatt
    16561 gatatattag aactagaaaa tgttattact aaagaaacac agcaggcgat taataaaact
    16621 gtcggtattc gtaaacaatt tacatatgat tacacagcct tagatattaa gattatcatg
    16681 ccaatgtttg atgacgcatt accttattac aaacaattta ttacagaagt aacgacattt
    16741 ttacatcaag aaaatgtacc agtaacagct tttggtaaag gagaaaatca ataatgaaac
    16801 agtataaagc gtatttaatc gatttagatg gcacaatgta tatgggaaca gatgagattg
    16861 atggagcaaa acaattcatc gattatttaa atgtaaaagg cattcctcat ttatacgtaa
    16921 ctaataattc aacaaaaaca cctgagcaag taactgaaaa attacgtgaa atgcacattg
    16981 atgctaaacc agaagaggtt gtaacgtcag cgttagccac tgctgattat atttcagaac
    17041 aatcaccagg agcatcagta tatatgttag gtgggagtgg tttaaatact gcgttaaccg
    17101 aagcgggact tgtcattaaa aatgacgagc atgttgatta tgtagttatt ggacttgacg
    17161 aacaagttac atatgaaaag cttgcgattg caacgttagg tgtaagaaat ggtgcaacat
    17221 ttatttctac aaatcctgat gtatcaattc ctaaagagcg tggtttatta cctggtaatg
    17281 gtgctattac aagtgttgta agtgtatcga caggtgtatc gccacaattt attggtaaac
    17341 cagaaccgat tattatggtt aaagcattag aaattttagg attagataaa tccgaagttg
    17401 ctatggtagg cgatttgtac gataccgata ttatgtctgg tattaacgta ggtatggata
    17461 cgattcatgt acaaacaggt gtatctacgt tagaagatgt gcaaaataaa aatgtgccac
    17521 caacgtattc ttttaaagat ttaaatgaag caatagctga attagaaaaa tagatatagt
    17581 cattttataa agtaggtgaa ttgatttggt aaaaatagtt gtttcgagga aaattccaga
    17641 taaattttat caacaattaa gtaaacttgg tgacgttgtt atgtggcaaa aatcattagt
    17701 gcctatgcct aaagatcaat ttgtgacagc acttcgtgac gcagatgctt gttttattac
    17761 attaagtgaa cagatcgatg cagaaatttt agcgcaatca ccaaatttaa aagtaattgc
    17821 gaatatggct gtaggatatg acaacatcga tgttgaaagt gcaacagcga ataacgtggt
    17881 tgtcacgaat acaccaaatg tacttactga aacaactgca gaattaggat ttacattaat
    17941 gcttgctata gcacgccgta ttgtagaagc tgaaaaatat gtagaagcag atgcatggca
    18001 aagctggggt ccttatttat tgtcaggtaa agatgtcttc aattcaacta ttggaatata
    18061 tggtatggga gatattggta aagcttttgc aagaaggttg caagggttta atactaatat
    18121 tctttatcat aatcgatcaa gacataaaga tgcagaggcg gactttaatg caacatatgt
    18181 ttcttttgaa acgttgttag cagaaagtga ttttatcatc tgtacagcgc cacttacaaa
    18241 agaaacacat cataaattta atgctgaagc atttgaacaa atgaaaaatg atgcaatttt
    18301 tattaatatc ggtagaggac aaattgtaga tgaaacagca ttaatcgatg cactagacaa
    18361 taaagaaatt ttagcatgtg gtttagatgt attagcaaat gaaccgattg atcatacaca
    18421 tccattaatg ggacgtgata atgttctgat tacaccacac attggtagcg catcagtaac
    18481 aacacgggac aatatgattc aattatgtat taataatata gaagcggtta tgacaaatca
    18541 ggtaccacat actccagtaa attgaaaata agtatggttt ttaatcattg gtaaaacaag
    18601 caaagcaatt gttaaatatg ttatgcttga cttgcgaatg tatatatttg cgctgatgat
    18661 aattcaataa cattataaac tcgtcatatt aatgtgatga gtttatttga ttttgacgta
    18721 tataacaaaa ttgttatata gactgtaatt gtttattgtt taaataacct ttaaaatgaa
    18781 tgatgttctc aacaaagtgt atcatttaca ataattgagt gatgacaacc aattactatg
    18841 ttgaatctat aaaattaaaa ttgaatttca ttatgaatat cgatataatg aattgtaaaa
    18901 aacaaatttt aaaacgtatt ttatattgaa cggtctttat aaaggcgttt ttactaagaa
    18961 ctttaagagg tgcaatatga aatctaaaag taaacagcca cctaataaat atgttgaagc
    19021 attcaaacca tatttattaa cactattgta tttggcaata tttattactt tatatttaat
    19081 ttatggcagt ggcgacacac acaataactt catttataat gagttctaat gagggagact
    19141 taatatgaca gatattatta acaagctgca agcgtttgcg gatgcaaatc cacaaagcat
    19201 tgctgttaga cacacaactg atgaattaac ttatcaacag ttaatggatg agtctagtaa
    19261 attagcacat cgattacaag gtagtaagaa accgatgatt ttattcggtc acatgtcacc
    19321 atatatgatt gttgggatga ttggtgccat taaagcagga tgtggatatg tacctgtaga
    19381 cacttcaatt cctgaagacc gtattaaaat gattattaac aaggttcaac cagagtttgt
    19441 atttaatacg actgatgaat catttgaaag tttagaaggc gaagtattta caatagaaga
    19501 tattaaaaca tctcaagacc cagtaatttt tgatagtcag attaaagata acgacacagt
    19561 atacacaatc tttacatctg gttctactgg ggaacctaaa ggtgttcaaa ttgaatatgc
    19621 aagtttagtt caatttactg agtggatgtt agaacttaat aaatcaggaa atgaacaaca
    19681 atggcttaac caagcgccat tttcatttga tttatctgta atggctattt atccatgttt
    19741 agcatcaggc ggtacattaa atcttgtaga taaaaacatg attaataaac ctaaattatt
    19801 aaatgaaatg ctaacagcaa caccgattaa catttgggta tcaacaccat catttatgga
    19861 aatgtgttta ttattaccaa cgcttaatga agaacaatat ggtagtctta acgaattctt
    19921 cttctgtggt gaaattctac ctcacagagc agcaaaagcg ttagtaaacc gtttcccaag
    19981 tgcgacgatt tacaacacat atggtccaac tgaagctacg gtagcagtta caagtattca
    20041 aattacacaa gaaatcttag atcaatatcc gacattacct gttggcgttg aaagaccagg
    20101 cgcaagatta tctactacag atgaaggtga acttgttatc gaaggtcaaa gtgtaagttt
    20161 aggatactta aaaaatgacc aaaaaacagc tgaagtattt aatttcgatg acggtattcg
    20221 tacatatcac actggtgata aagcgaagtt tgaaaatggt caatggttca ttcaaggtcg
    20281 tattgatttc caaatcaaat tgaatggcta cagaatggaa ttagaagaaa ttgaaacaca
    20341 attacgccag tctgagttcg taaaagaagc gattgttgta cctgtatata aaaatgataa
    20401 agttattcat ttaattggtg caattgtgcc aacgactgaa gttacggaca atgcagaaat
    20461 gactaaaaat attaaaaatg acttgaaatc acgcttacca gagtatatga ttcctagaaa
    20521 gtttgaatgg atggaacaat tgccattgac ttcaaatggt aaaattgaca gaaagaaaat
    20581 tgcagaggta attaacggat gattccatat ggtgatttta cattcttctt aattgcttta
    20641 attgcattat taccagtcat tatacttgga tttttaggta agcgaagtta catttataat
    20701 ggcgtagtta cagcatttat gattgtgtta atcttttctt ctgataaaca taatctgttt
    20761 gaccaaaagt atttaagtgt tcaattaatt agttttatta tttacgtcgt atggcaagtt
    20821 ttattgataa tgttttatta tcattcaaaa ccaaaaaata attcattttc aaaatttgta
    20881 actgtaatgg ttttatcaat attgccatta gcacttgtga aagtgttaca aagtacatgg
    20941 ttaggtggac atcagattca cttccatgaa agtaaattaa ttgaatttgt tggtttctta
    21001 ggaatttctt atgttacatt caaaagtgtg cagttaatta tggaaattcg tgatggttct
    21061 atcaaagaaa ttaaagtatg gaaattaatt caatttattt cattcttccc aacgatttca
    21121 tctggaccaa tcgatcgtta caaacgtttc gttaaagacg ataaaaaagt accaacaggc
    21181 aatgaatatc gtgaattagt attaaaagca attcacatga ttatgcttgg tttcttgtat
    21241 aaatatattg ttgcttactt tattaacaca tatgcaatca tgccgttaca attagactta
    21301 catggctttg tcaatttgtg gttatatatg tacgcataca gcttatattt attctttgac
    21361 tttgcaggtt atagtttatt tgcgatagca tttagttatt tattcggtat taaaacacca
    21421 ccaaacttcg ataaaccttt caaagcgaaa aatattaaag atttctggaa tagatggcat
    21481 atgacattat cattctggtt cagagattgt atttacatga gatctttatt ctacatgtct
    21541 cgtaaaaaat tattgaagag tcaatttgca atgtctaacg tggcattctt aatcaacttc
    21601 ttcataatgg gaatttggca tggtatcgaa gtgtattaca ttgtttatgg tttataccat
    21661 gcagcattgt ttataggtta tggctattat gaacgttggc gtaagaaaca tccgccacgt
    21721 tggcaaaatg gtttcacaac agcacttagc attgtgatta cattccactt tgtaacattt
    21781 ggctttttaa tcttctcagg taaacttata taataaagga gaatttaatt atggaattta
    21841 gagaacaagt attaaattta ttagcagaag tagcagaaaa tgatattgta aaagaaaatc
    21901 cagacgtaga aatttttgaa gaaggtatta ttgattcttt ccaaacagtt ggattattat
    21961 tagagattca aaataaactt gatatcgaag tatctattat ggactttgat agagatgagt
    22021 gggcaacacc aaataaaatc gttgaagcat tagaagagtt acgatgaaat taaaaccttt
    22081 tttacccatt ttaattagtg gagcggtatt cattgtcttt ctattattac ctgctagttg
    22141 gtttacagga ttagtaaatg aaaagactgt agaagataat agaacttcat tgacagatca
    22201 agtactaaaa ggcacactca ttcaagataa gttatacgaa tcaaacaagt attatcctat
    22261 atacggctct agtgaattag gtaaagatga cccatttaat cctgcaattg cattaaataa
    22321 gcataacgcc aacaaaaaag cattcttatt aggtgctggt ggttctacag acttaattaa
    22381 cgcagttgaa cttgcatcac agtatgataa attaaaaggt aagaaattaa catttattat
    22441 ttcaccacaa tggtttacaa accatggttt aacgaatcaa aactttgatg ctcgtatgtc
    22501 tcaaactcaa attaatcaaa tgttccagca gaaaaacatg tctactgaat taaaacgtcg
    22561 ttatgcacaa cgtttattac agtttccaca tgtacacaat aaagaatact tgaaatctta
    22621 tgctaaaaac cctaaagaaa ctaaagatag ttatatttct ggttttaaag agaatcaatt
    22681 gattaaaata gaagcgatta aatcattgtt tgcaatggat aaatctccat tagaacatgt
    22741 taaacctgct acaaaaccag acgcttcttg ggatgagatg aaacaaaaag cagttgaaat
    22801 tggtaaagct gatactacat cgaataaatt tggtattaga gatcaatact ggaaattaat
    22861 tcaagaaagt aagcgtaaag ttagacgtga ctacgaattc aatgttaatt ctccagaatt
    22921 ccaagattta gaattacttg taaaaacaat gcgtgctgct ggtgcagatg ttcaatatgt
    22981 aagtattcca tcaaacggtg tatggtatga ccacattggt atcgataaag aacgtcgtca
    23041 agcagtttat aaaaaaatcc attctactgt tgtagataat ggtggtaaaa tttacgatat
    23101 gactgataaa gaatatgaaa aatatgttat cagtgatgcc gtacacatcg gttggaaagg
    23161 ttgggtttat atggatgagc aaattgcgaa acatatgaaa ggtgaaccac aacctgaagt
    23221 agataaacct aaaaattaaa atacaaatag cacataactc aacgattttg attgagcgta
    23281 tgtgctattt ttatatttta aatttcatag aatagaatag taatatgtgc ttggatatgt
    23341 ggcaataata aaataattaa tcagataaat agtataaaat aactttccca tcagtccaat
    23401 ttgacagcga aaaaagacag gtaataactg attataaata attcagtatt cctgtctttg
    23461 ttgttattca taatatgttc tgttaactta atatctttat attagaatac ttgttctact
    23521 tctattacac caggcacttc ttcgtgtaat gcacgctcaa taccagcttt aagagtgatt
    23581 gtagaacttg ggcatgtacc acatgcacca tgtaattgta atttaacaat accgtcttcc
    23641 acgtcaatca atgagcagtc gccaccatca cgtaataaaa atggacgaag acgttcaata
    23701 acttctgcta cttgatcaaa catcgttgta tcttcagtag gcatcaggca agtctccttt
    23761 cgagagatga atttgataat ctttattagt atattataat agatagtgta ataaaaatct
    23821 atcaaaccaa tgggggataa atatatggag cacgtgagtg tggtagttta tggggcagat
    23881 gttatatgtg caagttgcgt taatgcgcca acatcgaaag atatttatga ctggctacag
    23941 ccgctattaa aaagaaaata cccaaatata tcatttaaat atacgtatat agatattaca
    24001 aaagataatg acaacttaac agatcatgat ttacaattta ttgaaagaat agaacaagat
    24061 gaactatttt atccattaat tacaatgaac gatgaatatg tagcagatgg ttatatacaa
    24121 acaaagcaaa tcactcgatt tatagaccaa aagcttgtga atgaataata tacacataac
    24181 taaaagcaat gtcagtaata gtgtgaactg acattgcttt ttaattttaa ccattgtgat
    24241 atttatataa ccaaagtacg ccggatttta aaatagaagc gagtcgtcct gttacagtgc
    24301 ggtccatgat atatgcaaaa ccttgcttat ctcctaatga cccaacgata ccttgtacct
    24361 ttagttccgg cattttgtca ggtaatggtt catttagcca ttgcttttta agcacatcgg
    24421 caatttgatc accttgaact tcggctaact gagcacttgg cgcatgtggt aaatcagcac
    24481 aatcaccaac tacatagacg ttacgatatg ttggtacttg atgatactgg ttaactatca
    24541 cgcgtccatt actatttata tcaatcggca agttacgaac aacttcaaca ggttgaattc
    24601 ctgctgtcca tacaactaaa tcaatatctt taggttcatc acagttatat attttaccag
    24661 gttcaacttt attaatattt gaatttggaa caacggtaac attatttttg gcgaaccatt
    24721 tcgcaacata cttacttaat ttttctggaa aatttcttaa aattcgcggc ccacggtcat
    24781 aaagatatat ttccaagtct gatctacttt ctcttaattc gctggcaagt tctatgccgc
    24841 ttaatccagc accaacgata ccgactttag caccttctgg tagttcacta atactatgga
    24901 aagtatcccg agcctttgag agtgtttgaa tactatgtgt atattcttcg gctcctggaa
    24961 cgttatgata tttatcttca catcctaaac caatgattag ctcatcataa tcaattttag
    25021 aattaccgac tgagacaatt tgagcatcta aatctatgtc gttaatttca ccataaactg
    25081 tattcacttg tggatgatta gggaatttca tacgaacatc tttatctgat ttcgtgcccg
    25141 cagctaaagc ataaaattct ggtttcaatc catgaaatgg catacgatca actaatgtga
    25201 ctgtataatc ttgtggtaaa gaagtagtta aaatgcgtga catgatacgc atattaccat
    25261 atccgccgcc taacaaaact aagtttttca taatgaagta ccccttttat atgttaatat
    25321 ttactttaat tttgaaaaga tcaaagtgca tagatatatt tgttaatgaa cagttataaa
    25381 atgcaagtgt ctttgattca tcaataaccc ct
//
LOCUS       DALAGD010000034        25361 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.034, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000034 DALAGD010000000
VERSION     DALAGD010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 25361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25361
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.034"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            120..1253
                     /locus_tag="O2701_001138"
     CDS             120..1253
                     /locus_tag="O2701_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HCZ4293042.1"
                     /translation="MINEQRLLNTFFELVQIDSETGNESTIQPILKEKFIALGLDVKE
                     DEAAKHPKLGANNLVCTMNSTIEEGEVPKLYLTSHMDTVVPAINVKPIVKDDGYIYSD
                     GTTILGADDKAGLAAMLEVLQVIKEQQIPHGQIQFVITVGEESGLIGAKELNSELLDA
                     DFGYAIDASADVGTTVVGAPTQMLISAKIFGKTAHASTPKEGVSAINIAAKAISRMKL
                     GQVDEITTANIGKFHGGSATNIVADEVILEAEARSHDPERIKTQVKHMTDVFETTASE
                     LGGKAVVTVEQSYPGFKINDNEAVVKIAQESARNLGLSANTIISGGGSDGSIINTFGI
                     PSVILGVGYEKIHTTNERMPIKSLNLLASQVLEIIKIVARHSK"
     gene            1321..2727
                     /gene="gndA"
                     /locus_tag="O2701_001139"
     CDS             1321..2727
                     /gene="gndA"
                     /locus_tag="O2701_001139"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCZ4293043.1"
                     /translation="MTQQIGVIGLAVMGKNLAWNIESRGYSVSVFNRSSEKTDLMVEE
                     SKGKNIHPTYSLEEFVNSLEKPRKILLMVQAGKATDATIDSLLPLLDDGDILIDGGNT
                     NYQDTIRRNKALAQSAINFIGMGVSGGEIGALTGPSLMPGGQEEAYNKVADILDAIAA
                     KAKDGASCVTYIGPNGAGHYVKMVHNGIEYADMQLIAESYAMMKELLGMSHEDIAQTF
                     KDWNAGELESYLIEITGDIFMKLDENKEALVEKILDTAGQKGTGKWTSINALELGIPL
                     TIITESVFARFISSIKEERVNASKELNGPKASFDGDKKDFLEKIRKALYMSKICSYAQ
                     GFAQMRKASEDNEWNLKLGDLAMIWREGCIIRAQFLQKIKDAYDNNPGLQNLLLDPYF
                     KNIVTEYQDALRDVVATGVQNGVPTPGFSSSINYYDSYRAADLPANLIQAQRDYFGAH
                     TYERKDKEGVFHTQWIEE"
     gene            2946..3317
                     /locus_tag="O2701_001140"
     CDS             2946..3317
                     /locus_tag="O2701_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCZ4293044.1"
                     /translation="MEKLNQVMLYVDDVEKAKAFWTETLEFVVVSETPLAEDYVAVEV
                     SPTKDAETSLTIMAKEFIEKHSPEVNLGTPSLMFKEKNFDALYSKLNDLGLTGHDIVE
                     MNGQRVFNFQDGQGNYFAVSD"
     gene            3256..3510
                     /locus_tag="O2701_001141"
     CDS             3256..3510
                     /locus_tag="O2701_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293045.1"
                     /translation="MVNVYLIFKMAKVTILQLVTKQSILIFIQTFILIFGLNFSMKVL
                     FLSFYAIVCTNLLTFLESVTKLVLYSGWKRFPMQKLTGGD"
     gene            3511..4530
                     /locus_tag="O2701_001142"
     CDS             3511..4530
                     /locus_tag="O2701_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCZ4293046.1"
                     /translation="MVTIKDVALKAGVSPSTVSRVIKGNQRISEATISKVKKVMEELN
                     YFPNTAARTLITNQTYKIGLVLKGSEEPIRLNPFYINVLLGISETCNQHGYGTQTTVS
                     NNMNDLMDEVYKMIKQRMVDAFILLYSKENDPIKQMLIDESMPFIVIGKPTSDIDHQF
                     THIDNDNILASENLTRHVIEQGVDELIFITEKGNFEVSKDRIQGFETIASQNKINYQI
                     IETSNEREVIFNYMQNLHTRLKDPNIKQAIISLDAMLHLAILSVLYELNIEIPKDVMT
                     ATFNDSYLTEIASPPQTCIDIKPRMLGQQAGAAILNILKNKAQDVIELVIIDTELKIR
                     KSTQR"
     gene            4546..6195
                     /locus_tag="O2701_001143"
     CDS             4546..6195
                     /locus_tag="O2701_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCZ4293047.1"
                     /translation="MNKQWWKEAVAYQVYPRSFNDSNHDGIGDLPGMIDKLDYLKDFG
                     IDVIWLSPMFKSPNDDNGYDISDYQEIMDEFGTMEDFDRLLKGVHDRGMKLILDLVVN
                     HTSDEHPWFIESKSSKDNPKRDWYIWQDPKPDGSEPNNWESIFNGSTWEYDANTEQYY
                     FHLFSKKQPDLNWGNPEVRDAVFEMMNWWFDKGIDGFRVDAITHIKKTFEAGDLPVPE
                     GKTYAPAFDVDMNQPGIQTWLQEMKDRSLSKYDIMTVGEANGVSPDDADDWVGEENGK
                     FNMIFQFEHLGLWNSGDSHFDVNSYKSVLNRWQKQLENKGWNALFIENHDQPRRVSTW
                     GDDDKYWYESATSHAAVYFLQQGTPFIYQGQEIGMTNYPFESIETFNDVAVKNDYQIV
                     KAQGGDVDALLAKYKDENRDNSRTPMQWDDTLNGGFTNGEPWFPVNPNYKTINVAQQL
                     EDEHSVLQFYKDLIQLRKSNDVYVYGQFDLVDAENSQVFAYTRTLNEKQVLIVGNLTN
                     HEAELTVPFDLSHGEVKLFNYDAKVNLKQLRPYEACVIELN"
     gene            complement(6278..7144)
                     /locus_tag="O2701_001144"
     CDS             complement(6278..7144)
                     /locus_tag="O2701_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCZ4293048.1"
                     /translation="MDVIKQIQQAIVYIEDRLLEPFNLQELSDYVGLSPYHLDQSFKM
                     IVGLSPEAYARARKMTLAANDVINGATRLVDIAKKYHYANSNDFANDFSDFHGVSPIQ
                     ASTKKDELQIQERLYIKLSTTERAPYPYRLEETDDISLVGYARFIDTKYLSHPFNVPD
                     FLEDLLIDGKIKELRRYNDVSPFELFVISCPLENGLEIFVGVPSERYPAHLESRFLPG
                     KHCAKFNLQGEIDYATNEAWYYIESSLQLTLPYERNDLYVEVYPLDISFNDPFTKIQL
                     WIPVKQSPYDED"
     gene            complement(7374..8858)
                     /locus_tag="O2701_001145"
     CDS             complement(7374..8858)
                     /locus_tag="O2701_001145"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCZ4293049.1"
                     /translation="MSTKNKHIPCLITIFGATGDLSHRKLFPSIFHLYQQDNLDEHIA
                     IIGIGRRDITNDDFRNQVKSSIQKHVKDTNKIDAFMEHVFYHRHDVSNEESYQELLDF
                     SNELDSQFELKGNRLFYLAMAPQFFGVISDYLKSSGLTDTKGFKRLVIEKPFGSDLKS
                     AEALNNQIRKSFKEEEIYRIDHYLGKDMVQNIEVLRFANAMFEPLWNNKYISNIQVTS
                     SEILGVEDRGGYYESSGALKDMVQNHMLQMVALLAMEAPISLNSEDIRAEKVKVLKSL
                     RHFQSEDVKKNFVRGQYGEGYIDGKQVKAYRDEDRVADDSNTPTFVSGKLTIDNFRWA
                     GVPFYIRTGKRMKSKTIQVVVEFKEVPMNLYYETDKLLDSNLLVINIQPNEGVSLHLN
                     AKKNTQGIETEPVQLSYSMSAQDKMNTVDAYENLLFDCLKGDATNFTHWEELKSTWKF
                     VDAIQDEWNMVDPEFPNYESGTNGPLESDLLLARDGNHWWDDIQ"
     gene            9171..10091
                     /gene="rnz"
                     /locus_tag="O2701_001146"
     CDS             9171..10091
                     /gene="rnz"
                     /locus_tag="O2701_001146"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HCZ4293050.1"
                     /translation="MEVTFFGTSAGLPTKERNTQAIALNLEPYSNSIWLFDVGEGTQH
                     QILHHAIKLGKVTHIFITHMHGDHIFGLPGLLSSRSFQGGEQKPLTLVGPKGIKAYVE
                     MSMNLSESHLNYPITYIEIDDHLTYHHDGFTVEAHLLNHGIPSYGYRVMAPETTGTIN
                     VEALKNIGLEPGPKYQEVKSHDTFEHNGQVYQSKDFRGESKQGPVVAIFGDTKPCSNE
                     RVISRDADVMVHEATYIDGEKHLANNYHHSHIEDVFALIKEANVKRTLITHLSNRYNT
                     EDINEIYQTLIQNEDTPNFNFVKDFDSFKI"
     gene            complement(10196..11011)
                     /gene="proC"
                     /locus_tag="O2701_001147"
     CDS             complement(10196..11011)
                     /gene="proC"
                     /locus_tag="O2701_001147"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCZ4293051.1"
                     /translation="MKLVFYGAGNMAQAIFTGIINSSNLDANDIYLTNKSNEQALKAF
                     AEKLGVNYSYDDATLLKDADYVFLGTKPHDFDALATRIKPHITKDNCFISIMAGIPID
                     YIKQQLECQNPVARIMPNTNAQVGHSVTGISFSNNFDPKSKDEINDLVKAFGSVIEVS
                     EDHLHQVTAITGSGPAFLYHVFEQYVKAGTKLGLEKEQVEESIRNLIIGTSKMIERSD
                     LSMAQLRKNITSKGGTTQAGLDTLSQYDLVSIFEDCLNAAVDRSIELSNIEDQ"
     gene            11153..11908
                     /locus_tag="O2701_001148"
     CDS             11153..11908
                     /locus_tag="O2701_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCZ4293052.1"
                     /translation="MKGQHFIVTGGTSGLGLSIVRKLLENKVHVTLLVRDVDKATRIF
                     EQELGKTINVIPCDLNDLKSIQALQFEDNTSFDGFIYSAGLGYFKSISDHSFSEMIET
                     YQLNLISFNVLYTVLRPYLTSNAHIVGISSQAAFSTQANAAHYGASKAGFYALMNALR
                     LESPNLHIMTVNVGPIDTPFHQKADPSMKYAKKMGEIMLDANQLAEDIIYGIKTKQLE
                     INRPKWMHHALKMYQIAPRFFERCFPKLFKNKA"
     gene            11924..12172
                     /locus_tag="O2701_001149"
     CDS             11924..12172
                     /locus_tag="O2701_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293053.1"
                     /translation="MNCQSESVVRLCVRYAEQLSVFEEFTVLDILGDISVDQISDGTL
                     YYTCEKFKLLVLQGNVLGVQIITNNDESTCEVKYRKMF"
     gene            complement(12388..13296)
                     /locus_tag="O2701_001150"
     CDS             complement(12388..13296)
                     /locus_tag="O2701_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCZ4293054.1"
                     /translation="MQKNILKSGISLSELGLGCMSLGTDLKKAEQIIDCAVENGITYF
                     DTADMYDKGINESVVGKALLKYQQRDDIFIGTKVGNRLTKDGSTTWDPSKSYIKEAVK
                     GSLKRLGIDHIDLYQLHGGTIDDPLDETISAFDELKQEGIIRAYGISSIRPNVIDYYL
                     KHSQIETIMSQFNLIDNRPESLLDAIHNNDVKVLARGPVSKGLLTSNSVNVLDNKFKD
                     GIFDYSHDELGETIASIKEIESNLSALTFSYLTSHDVLGSIIVGASSVDQLKENIENY
                     HTKVSLDQIKTARARVKDLEYTNHLV"
     gene            13378..13920
                     /locus_tag="O2701_001151"
     CDS             13378..13920
                     /locus_tag="O2701_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCZ4293055.1"
                     /translation="MDLNEKTIDRTVIYNGKIVDVEIHTVTLPNGETSTRELVYHNGA
                     VAVCAVTPKKEVVLVKQYRKPVEKPLLEIPAGKLEDDEDRVEAAKRELEEETGYIAKE
                     LTHVVDMYGSPGFCDEQLSIYFTDNLEEGTVHLDEDEFVEVIKVPIENVKSMLMNKEI
                     EDAKTIIALQHLLLNYNHSK"
     gene            14025..14474
                     /locus_tag="O2701_001152"
     CDS             14025..14474
                     /locus_tag="O2701_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCZ4293056.1"
                     /translation="MEERLNRVKQQLQQSSYKLTPQREATVRVLIENEKDHLSAEDVY
                     LKVKDKAPEIGLATVYRTLELLAELKVVDKINFGDGVARFDLRKEGAKHFHHHLVCME
                     CGRVDEIDEDLLPEVENRVENEFNFKILDHRLTFHGVCETCQAKGKG"
     gene            14523..15410
                     /gene="xerD"
                     /locus_tag="O2701_001153"
     CDS             14523..15410
                     /gene="xerD"
                     /locus_tag="O2701_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCZ4293057.1"
                     /translation="METIIEEYLRFIQIEKGLSSNTIGAYRRDLKKYQDYMTEHHISH
                     IDFIDRQLIQECLGHLIDQGQSAKSIARFISTIRSFHQFAIREKYAAKDPTVLLDSPK
                     YDKKLPDVLNVDEVLALLETPDLNKINGYRDRTMLELLYATGMRVSELIHLELENVNL
                     IMGFVRVFGKGDKERIVPLGDAVIEYLTTYIETIRPQLLKKTVTEVLFLNMHGKPLSR
                     QAIWKMIKQNGVKANIKKTLTPHTLRHSFATHLLENGADLRAVQEMLGHSDISTTQLY
                     THVSKSQIRKMYNQFHPRA"
     gene            complement(15488..15994)
                     /locus_tag="O2701_001154"
     CDS             complement(15488..15994)
                     /locus_tag="O2701_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HCZ4293058.1"
                     /translation="MQQALINFYYQFHTKQHYFLCHDILEDAWKAENNYSKQDAVVSL
                     ILFATACYHYRRNNLKGAYKSFNKSKEIIQNAKDRDALYLNLNDYQLLIEQQIAKLNA
                     AKPFSSVILPITPVFERIIKANYPDYDYNQETATDPFIVDHHMRRDRSEVIAAKEEAI
                     QLRKHRRN"
     gene            16086..16817
                     /locus_tag="O2701_001155"
     CDS             16086..16817
                     /locus_tag="O2701_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HCZ4293059.1"
                     /translation="MYEVKLDAFNGPLDLLLHLIQKFEIDIYDIPMQALTEQYMQYVH
                     AMKQLEINIASEYLVLASELLMIKSKMLLPQSTSDMDVDDDPREDLVGRLIEYQNYKE
                     YTAILNDMKEERDFYFTKRPTDLSHLETDESWDPNHTIDLTELIVAYQRVKNRVELNT
                     PKSVEIRKETFTIQQATEQVTSRLKDKDHFNFFSLFTFSEPIEQVVTHFLAILEMSKA
                     GIINIEQQRNFEDINIIRGVNYHFG"
     gene            16810..17352
                     /gene="scpB"
                     /locus_tag="O2701_001156"
     CDS             16810..17352
                     /gene="scpB"
                     /locus_tag="O2701_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HCZ4293060.1"
                     /translation="MDNHGILESLLFTAGDEGLDEKQLLEILDMSKDQLVELIENYSS
                     HGLMIQRFGMTYVLTTKKEAATYIEQLIEQKSQMKLSQAAMEVLSIIAYNQPLSRSDI
                     ELIRSINSDGAVKTLIAKGLVEAKVVNEQRSQQLITTDLFLNVFGISNIEDLPTTEED
                     DEEMDAFFSNLVNQKGENND"
     gene            17345..18082
                     /locus_tag="O2701_001157"
     CDS             17345..18082
                     /locus_tag="O2701_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCZ4293061.1"
                     /translation="MTKELERLQKRIANSGYTSRRKAETLISEGKVKVNGTTVTELGT
                     KVKPSDTIEVEGIKIELEDKIYILFHKPTQVITSVSDDRGRTVVTDYFKDIEARIYPV
                     GRLDYDTSGLLLLTNDGEFTNLMTHPRYQIKKKYVAKLKGYLMREEVKALEKGIELED
                     GMTQPAEVKVKKQDKDKNTTLVEITITEGRNRQVRRMFEHFGHQVTKLSRIEYGPLNV
                     VGLNAGEGRVLTPHEVKVMRHLAEHGK"
     gene            18215..18940
                     /gene="srrA"
                     /locus_tag="O2701_001158"
     CDS             18215..18940
                     /gene="srrA"
                     /locus_tag="O2701_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator SrrA"
                     /protein_id="HCZ4293062.1"
                     /translation="MSNEILIVDDEDRIRRLLKMYLERESFEIHEASNGQEAYELAME
                     NNYACILLDLMLPEMDGIQVATKLREHKQTPIIMLTAKGEETNRVEGFESGADDYIVK
                     PFSPREVVLRVKALLRRTQSTTVEQSEPHARDVIEFKHLEIDNDAHRVLADNQEVNLT
                     PKEYELLIYLAKTPNKVFDREQLLKEVWHYEFYGDLRTVDTHVKRLREKLNRVSSEAA
                     HMIQTVWGVGYKFEVKSNDEPAK"
     gene            18921..20672
                     /gene="srrB"
                     /locus_tag="O2701_001159"
     CDS             18921..20672
                     /gene="srrB"
                     /locus_tag="O2701_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SrrB"
                     /protein_id="HCZ4293063.1"
                     /translation="MMSRLNSVVIKLWLTIILIVTTVLILLSIALITFMQYYFTQETE
                     NAIREDARRISSLVEQSHNKEEAIKYSQTLIENPGGLMIINNKHRQSTASLSNIKKQM
                     LNEVVNNDHFDDVFDKGKSVTRNVTIKEKGSSQTYILLGYPTKAQKNSHSKYSGVFIY
                     KDLKSIEDTNNAITIITIITAVIFLTITTVFAFFLSSRITKPLRRLRDQATRVSEGDY
                     SYKPSVTTKDEIGQLSQAFNQMSTEIEEHVDALSTSKNIRDSLINSMVEGVLGINESR
                     QIILSNKMANDIMDNIDEDAKAFLLRQIEDTFKSKQTEMRDLEMNARFFVVTTSYIDK
                     IEQGGKSGVVVTVRDMTNEHNLDQMKKDFIANVSHELRTPISLLQGYTESIVDGIVTE
                     PDEIKESLAIVLDESKRLNRLVNELLNVARMDAEGLSVNKEVQPIAALLDKMKIKYRQ
                     QADDLGLNMTFNYCKKRVWSYDMDRMDQVLTNLIDNASRYTKPGDEIAITCDENESED
                     ILYIKDTGTGIAPEHLQQVFDRFYKVDAARTRGKQGTGLGLFICKMIIEEHGGSIDVK
                     SELGKGTTFIIKLPKPE"
     gene            20917..21846
                     /locus_tag="O2701_001160"
     CDS             20917..21846
                     /locus_tag="O2701_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HCZ4293064.1"
                     /translation="MKKFIGSVLATTLILGGCSTMENESKKDTKTETKSVPEEMEASK
                     YVGQGFQPPAEKNAIEFAKKHRKEFEKVGEQFFKDNFGLKVKATNVVGKDDGVEVYVH
                     CEDHGIVFNASLPLYKDAIHQKGSMRSNDNGDDMSMMVGTVLSGFEYRAQKEKYDNLY
                     KFFKENEKKYQYTGFTKEAINKTQNVGYKNEYFYITYSSRSLKEYRKYYEPLIRKNDK
                     EFKEGMERARKEVNYAANTDAVATLFSTKKNFTKDNTVDDVIELSDKLYNLKNKPDKS
                     TITIQIGKPTINTKKAFYDDNRPIEYGVHSKDE"
     gene            21839..23865
                     /locus_tag="O2701_001161"
                     /pseudo
     CDS             21839..23865
                     /locus_tag="O2701_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500098.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23908..25113)
                     /locus_tag="O2701_001162"
     CDS             complement(23908..25113)
                     /locus_tag="O2701_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCZ4293065.1"
                     /translation="MWIEKFKNKNNETKYRYYEKYKDPYTDKWKRVSVVLNKNTKQSQ
                     KEAMFRLEEKIKEKLNNKSSSELKTLTFHALLDEWLEYHIKTSGSKLTTLNNIKIRIR
                     NIKRYSSENLLLNKLDTKYMQIFINKLSDIYSQNQVTRQLGDMKGAIKYAVKFYNYPN
                     EYLLTNVKIPKRRKTIEDIEKDESKMYNYLEMNQVLQIRDHILNDNKLHKRNRILIAS
                     ILEVQALTGMRIGELQALQEKDIDLLNKTINITGTIHRIKYEEGFGYKDTTKTISSKR
                     SISINSRTVEIFKKIILENKMLKRWNSSYVDRGFIFTTKKGNPLCNNQIAGVLKKTTK
                     ALNMNKKVTTHTFRHTHITLLVEMNVSLKAIMKRVGHVDEKTTIRIYTHVTEKMDREL
                     TQKLENIPS"
     gene            25239..>25361
                     /locus_tag="O2701_001163"
     CDS             25239..>25361
                     /locus_tag="O2701_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCZ4293066.1"
                     /translation="MTQFLGALLLTGVLGYIPYKYLTMIGLVSEKNKVINTPVLL"
BASE COUNT         9030 a         3563 c         4599 g         8169 t
ORIGIN      
        1 ataatatgac acaatttaca aacctgataa atctgtgtaa cgtcaattac tgtaacattt
       61 tatttcatca atttttaccg tttggcgtac atgaaaattt tgataaaagg ggttccatta
      121 tgattaatga gcaaagatta cttaatacgt tttttgaact tgtacaaatc gattctgaga
      181 cagggaatga atcaacaatc caacctattt taaaagaaaa attcatagca ttagggttag
      241 atgttaaaga agatgaagcg gctaaacatc ctaaattagg tgccaataat ctagtgtgta
      301 caatgaatag tactatcgaa gaaggcgaag tacctaaatt atatttgacg agccatatgg
      361 atactgttgt acccgcaatt aatgtaaagc caattgtaaa agatgacggc tacatatact
      421 ctgatggtac gactattcta ggtgcagatg ataaagcagg attagcagcg atgcttgaag
      481 ttttgcaagt gataaaggaa caacaaatcc cacatggaca aattcaattt gtgattactg
      541 ttggagaaga atcaggttta attggtgcta aagaattaaa ttcagagttg ttggacgcgg
      601 actttggtta tgctattgat gcaagtgctg atgtcggtac tacggttgta ggtgcaccga
      661 cgcaaatgtt aatttcagct aaaatttttg gcaaaacggc tcatgcaagt acgccaaaag
      721 agggtgttag tgctattaat attgcggcga aagcaattag tcgaatgaaa ttaggacaag
      781 ttgatgaaat aactactgcc aatattggta agtttcatgg cggttcagct acgaatattg
      841 ttgcagatga agtcatttta gaagcagagg cacgttctca cgatccagaa agaataaaaa
      901 cacaagtcaa acatatgact gatgtgtttg aaactactgc atcagaatta ggtgggaaag
      961 cagtagttac agtggagcaa agttatccag gatttaaaat taatgataat gaagcggttg
     1021 ttaaaattgc acaagaaagt gctcgtaatt taggattatc tgcaaataca attatttctg
     1081 gtggcggttc agacggcagt attattaata catttggtat tccttcagtc attttaggag
     1141 tgggttatga aaaaatccat acaacaaatg agagaatgcc gattaaatca ttgaacttgc
     1201 ttgcaagtca agtgttagaa ataatcaaaa tagttgcgcg acattcgaaa tgatttagtg
     1261 tagtgtatgt ctattttgtg atacaatact aatgaaaaat caacaagtga ggtaagtaat
     1321 atgacacaac aaattggagt tattggttta gccgttatgg gtaaaaacct agcttggaat
     1381 attgaatcac gcggatatag tgtatctgta tttaaccgct caagtgaaaa aactgattta
     1441 atggttgaag aatcaaaagg gaaaaatatt catccaacgt attcattgga agaatttgtt
     1501 aattctttag aaaaaccacg taaaatttta ttaatggttc aagcaggtaa agctacggac
     1561 gcaacgattg atagtttgtt acctttatta gatgatggag atattttaat tgatggtggt
     1621 aatacaaact atcaagatac tatcagacgt aataaagcat tagcacaaag tgcaatcaac
     1681 tttattggta tgggcgtttc tggtggtgaa attggagcgt taactggtcc gtcattaatg
     1741 ccgggtggac aagaagaagc ttataacaaa gttgcagata ttcttgatgc aattgctgca
     1801 aaggcaaaag acggtgcttc atgtgttact tatattggac ctaatggtgc tggacactat
     1861 gtgaaaatgg tacacaatgg tatcgaatat gctgacatgc aattaattgc agaaagttat
     1921 gctatgatga aagaattatt aggcatgtct catgaagaca ttgctcaaac gttcaaagac
     1981 tggaatgctg gtgaattaga aagttattta attgaaatca ctggtgatat tttcatgaaa
     2041 ttagatgaaa ataaagaagc attagttgaa aaaattcttg atactgcagg tcaaaaaggt
     2101 acaggaaaat ggacttcaat taacgcatta gaattaggta ttccattaac aatcattact
     2161 gaatctgtat ttgcacgttt tatctcttca atcaaagaag agcgcgtaaa tgcttctaaa
     2221 gaattaaatg gtccaaaagc atcatttgat ggtgacaaga aagacttttt agaaaaaatt
     2281 cgtaaagcat tatatatgag taaaatttgt tcttatgcac aaggtttcgc gcaaatgcgt
     2341 aaagcaagtg aagataatga atggaattta aaacttggtg atttagctat gatttggaga
     2401 gaaggttgca ttatccgtgc acaattctta caaaagatca aagatgctta cgataataat
     2461 ccaggattac aaaacttatt gctagatcct tactttaaaa acatcgttac tgaatatcaa
     2521 gatgcgttac gtgatgttgt cgcaactggt gttcaaaatg gtgtaccaac acctggtttc
     2581 tcatcaagta ttaattacta tgatagttac cgtgctgctg atttacctgc aaacttaatt
     2641 caagcacaac gtgactactt cggtgctcat acgtacgaaa gaaaagataa agaaggcgta
     2701 ttccatacac aatggattga agaataatat tgattaaagg atagttgaaa gatactatcc
     2761 ttttttatat cttttgataa atataaaaga gagtgtgata gagtagctac aacctataaa
     2821 aaattacaca agtattatcc ttgaccttaa aagcatcccg ccatattttt aagcattttg
     2881 tgaagtaata aaatatttac tgtgttatga tagcatgtac tatagagaaa tagaggtact
     2941 attttatgga aaagttaaat caagttatgt tatatgttga tgatgtcgaa aaagcgaaag
     3001 cattttggac agaaacttta gaatttgttg ttgtaagtga aacaccgtta gctgaggact
     3061 atgttgcagt tgaagtatca cctaccaaag atgctgaaac gtcattgacg attatggcaa
     3121 aagaatttat tgaaaagcat agcccggaag tgaacctagg aacaccatct ttaatgttta
     3181 aagagaaaaa ctttgatgca ttatactcaa aattaaatga tttaggttta actgggcatg
     3241 atatagtaga aatgaatggt caacgtgtat ttaattttca agatggccaa ggtaactatt
     3301 ttgcagttag tgactaaaca atcgatttta atatttatac aaacctttat attgattttc
     3361 ggattgaatt tcagtatgaa ggttttattt ttatcgtttt atgcaatcgt ttgcacaaat
     3421 cttttgactt ttttagaaag tgtgactaaa ctggtattat acagtggttg gaagcgtttt
     3481 ccaatgcaga aattaacagg aggtgattaa atggttacga ttaaagatgt tgcactaaaa
     3541 gccggtgttt ctccttcgac agtttcaaga gttataaaag gaaatcaacg tattagcgaa
     3601 gcgacaattt caaaagtgaa gaaagttatg gaagaattga attattttcc taataccgct
     3661 gctagaactt taattacaaa ccaaacatat aaaattggtt tagtgttaaa agggtctgag
     3721 gagcctattc gactgaatcc attctacatc aatgtattgc taggaatttc tgaaacgtgt
     3781 aaccagcatg gctatggtac acaaacgaca gtctcaaata atatgaatga tttaatggat
     3841 gaagtttata aaatgattaa acaacgaatg gttgatgcgt ttatactgct ctattcaaaa
     3901 gaaaatgatc cgattaaaca aatgttaatt gatgaaagca tgccatttat tgtgattggt
     3961 aagcctacat cggatataga tcatcaattt acacatatag ataatgataa tatattagct
     4021 tctgaaaatt tgacacgaca tgttattgaa caaggtgtag atgaattaat atttattaca
     4081 gaaaaaggaa attttgaagt ttcaaaagat agaattcaag gatttgaaac gatagcttct
     4141 caaaataaaa ttaattatca aattattgag actagtaatg aaagagaagt tatttttaat
     4201 tacatgcaaa atctacatac gcgtttgaag gacccaaata ttaaacaggc aatcatttcg
     4261 ttagatgcta tgttacattt agcgatttta agtgtcctat atgaacttaa tattgaaatt
     4321 ccgaaagatg taatgacagc aacgttcaat gattcttatt taaccgaaat tgcgtcacca
     4381 ccacaaacct gtattgacat caagcctcga atgttaggtc aacaggctgg tgcagcaatt
     4441 ttaaatatat tgaaaaacaa agcacaggat gttattgaac tcgtcattat agatacagaa
     4501 ttaaaaataa gaaaatcaac acagcgatag caaaggagtt ttaaaatgaa taagcaatgg
     4561 tggaaagaag cagtagcata tcaagtatat ccaagaagtt ttaatgatag taatcacgat
     4621 ggtattgggg atttacctgg aatgattgat aaattggact acttaaaaga tttcggtatc
     4681 gatgtcattt ggctcagtcc aatgtttaaa tcacctaatg atgacaatgg ttatgatatt
     4741 agtgactacc aagagattat ggatgaattt ggaacgatgg aagactttga tcgtttatta
     4801 aaaggtgttc atgatagagg catgaagctt attttagatt tagttgtaaa tcatacatct
     4861 gatgaacatc cttggtttat agaatccaaa tctagtaaag acaatcccaa acgtgattgg
     4921 tacatttggc aagatccaaa gccagatggc tctgaaccta acaactggga aagtatattt
     4981 aatggatcta catgggaata tgatgctaat actgagcaat attatttcca tttattcagt
     5041 aaaaaacaac ctgatttgaa ttggggtaat ccggaagtta gagatgctgt atttgaaatg
     5101 atgaactggt ggtttgataa aggcattgat ggatttagag tagatgcaat tacgcatatt
     5161 aagaagacgt ttgaagcggg tgacttacct gtacctgagg gtaaaacata tgccccagca
     5221 tttgatgtag atatgaatca gccaggtata caaacttggt tacaagagat gaaagatcgc
     5281 tcattaagta agtatgacat tatgactgtt ggtgaagcga atggtgtaag ccctgatgat
     5341 gctgatgact gggtcgggga agaaaatggt aaatttaata tgatattcca atttgaacat
     5401 ttgggactgt ggaatagtgg tgattctcac tttgatgtaa attcgtataa atctgtatta
     5461 aatagatggc aaaaacaact tgaaaataaa ggttggaatg cgttgtttat tgaaaatcac
     5521 gaccaaccac gacgtgtatc gacgtggggt gacgatgaca agtattggta tgaatcagca
     5581 acaagtcatg cagctgttta tttcttgcaa caaggtacgc cattcattta tcaaggtcaa
     5641 gaaattggta tgacgaatta tccatttgaa agtattgaaa cgtttaacga tgttgctgtt
     5701 aaaaatgact atcaaatagt gaaagctcaa ggtggagatg tagacgcttt acttgcgaaa
     5761 tataaagatg agaaccgaga taattctcgc acaccaatgc aatgggatga tacgttaaat
     5821 ggaggattta caaatggtga accgtggttc ccagtgaatc cgaattataa aactatcaat
     5881 gttgcacaac aattagaaga tgagcattca gtattacaat tttataaaga tttaattcaa
     5941 ttaagaaagt ctaatgatgt atacgtatat ggtcaatttg atttagtaga tgctgaaaat
     6001 tcacaagttt ttgcgtacac gagaacatta aatgaaaagc aagttcttat tgtaggtaat
     6061 cttactaacc acgaagcaga attaactgta ccatttgatt taagccatgg agaagtaaag
     6121 ctatttaatt atgatgccaa agttaattta aaacagttac gtccatatga agcatgtgtt
     6181 atcgaactaa attaacatat taatatgccc cctaatagaa tctaaggcca tttgtagacc
     6241 tatagatata ttagggggct tctttgtttt ttattattta atcttcgtca taaggactct
     6301 gtttaacagg aatccaaagc tgaattttag tgaatgggtc attaaatgaa atatcgagag
     6361 ggtacacttc aacatataaa tcatttcgtt catatggcaa tgttaactgc aaacttgatt
     6421 caatatagta ccaagcttca ttagttgcat aatcaatttc accttgtaaa ttgaatttcg
     6481 cacaatgttt gccaggtaaa aatcgacttt ctaagtgtgc aggataacgt tcacttggta
     6541 cacctacaaa tatttctaaa ccattttcaa gaggacaact aataacaaat agttcaaatg
     6601 gactaacgtc attatatcgt cgtaactctt taattttacc atcaatgagc aagtcttcta
     6661 aaaaatccgg aacattaaaa ggatgtgaca aatacttagt gtctataaat cgtgcatatc
     6721 caaccaatga aatatcatct gtctcttcta atctgtatgg ataaggtgct ctctcagtag
     6781 ttgataattt gatatataat cgctcttgaa tttgtaattc atctttttta gtagaggctt
     6841 gaataggtga tacgccgtga aaatcactaa aatcatttgc aaaatcattt gaatttgcat
     6901 agtgatattt tttagcgata tctacaagtc gtgtagcacc attaatcaca tcatttgcag
     6961 cgagtgtcat tttacgcgcg cgtgcataag cttctggaga taagccgaca atcattttaa
     7021 atgattgatc aagatggtat ggcgaaagac caacgtaatc acttaattct tgcaaattga
     7081 aaggctctaa taaacgatct tcaatataaa caattgcctg ttgtatttgc ttgataacgt
     7141 ccaaaacttt cactccaatt aattcaatca tttattttta ttctacatta tttctataaa
     7201 ttatacaccc atttgttcaa tgattattaa aatagttttg ggcattgtaa aatataattt
     7261 cataatatag tctagaaaaa aagcgaatga tagaacaatt gatttacttg attcgtaatc
     7321 aatccttgtc attcgctcat ttatttttgt ttaacatgtg cgttttaatt caattattga
     7381 atatcgtccc accaatggtt accatcacga gcaagtagta aatcactttc taatggacca
     7441 ttagtacctg attcatagtt agggaattct ggatcaacca tattccattc atcttgaatt
     7501 gcatcaacaa atttccatgt tgattttaat tcttcccagt gcgtgaagtt agtggcatca
     7561 cctttaagac aatcaaataa tagattttca tatgcatcta cagtattcat tttatcttga
     7621 gcgctcattg agtaagacaa ttggacaggt tctgtttcga taccttgtgt atttttctta
     7681 gcatttagat gtaaagatac accttcatta ggttggatat tgattactaa taggtttgaa
     7741 tctaacagtt tatcagtttc atagtataag ttcattggta cttctttaaa ttcaacgaca
     7801 acttgaattg ttttagattt catacgttta ccagtacgaa tatagaatgg tacaccagcc
     7861 catctaaagt tatcaattgt taatttacct gaaacaaagg taggtgtgtt agagtcatct
     7921 gcaacgcgat cttcatcacg gtatgcttta acttgtttac catcgatata gccttcgcca
     7981 tattgaccac gaacaaagtt ctttttaaca tcttcagatt ggaaatgacg cagtgattta
     8041 agtactttta ctttctcagc acggatatct tcactattta aactaatagg tgcttccata
     8101 gctaataatg caaccatttg taacatgtgg ttttgcacca tatcttttag cgcgccactt
     8161 gattcataat aaccaccacg atcttcaaca cctagtattt cagaagatgt aacttggatg
     8221 tttgaaatat atttgttatt ccataatggt tcaaacatcg cattcgcaaa acgtaatacc
     8281 tcgatatttt gaaccatgtc ttttcctaaa tagtggtcaa tacgataaat ttcttcttct
     8341 ttaaatgatt tacgaatttg attgtttaat gcttcggctg attttaaatc actaccgaat
     8401 ggtttttcga taacaaggcg tttaaatcct tttgtatcag taagaccaga agattttaga
     8461 taatcagaaa taacgccaaa gaattgtggt gccattgcta aatagaatag tcgattacct
     8521 tttaattcaa attggctatc taattcatta ctaaaatcta gtaattcttg atagctttct
     8581 tcattactaa catcatgtct atgatagaag acatgttcca taaacgcgtc aattttgttt
     8641 gtatctttta cgtgcttttg aattgatgat tttacttgat tacggaaatc atcattagta
     8701 atgtcacgac gtccaatacc gatgatggca atatgttcat ctaaattgtc ttgttggtag
     8761 agatggaata ttgatggaaa caacttacga tggcttaagt caccagttgc accaaagatt
     8821 gtgattaaac atgggatgtg tttgttttta gtactcaaga ttaaaacctc aattcttaat
     8881 tagatatatg atttattatt ataagataat ccattcagta ggtcatacaa tatgctcgac
     8941 tgtatgcagt gtcttaaatg aaatatcgat tcatgtatca tgtttaatgt gatacttatt
     9001 aatgataagt ataacgtaat tatcaaaatt tatatagtta tgtctacgtt aaagttagaa
     9061 aaattaacta gcaaagacga atttttaaca gattttgatt caagtataaa ttaaaactaa
     9121 attgatacaa attttatgat aaaatgaatt gaagaaaagg aggggcatat atggaagtta
     9181 cattttttgg aacgagtgca ggtttgccta caaaagagag aaatacacaa gcaatcgcct
     9241 taaatttaga accatattcc aattccatat ggcttttcga cgttggtgaa ggtacacagc
     9301 accaaatttt acatcatgca attaaattag gaaaagtgac acatatattt attactcata
     9361 tgcatggcga tcatattttt ggtttgccag gattactttc tagtcgttct tttcagggcg
     9421 gtgaacagaa gccgcttaca ttggttggac caaaaggaat taaagcatat gtggaaatgt
     9481 ctatgaattt atcagagtca catttaaact atccaattac atatattgaa atcgatgatc
     9541 atctgacata tcatcatgat ggttttactg tagaggcgca tttacttaat catggtattc
     9601 catcatatgg atatagagta atggcacctg aaactacagg tacaatcaat gttgaagcgt
     9661 taaaaaacat tggtcttgaa ccaggtccta aatatcaaga agtaaaatct catgatacat
     9721 ttgaacataa tggtcaagtt tatcaatcaa aagattttag aggtgagtcc aaacagggtc
     9781 cagttgtagc aatttttggt gatacaaaac catgttctaa tgaacgtgtg atttctagag
     9841 atgctgatgt gatggtacat gaagcgacct atatagatgg tgaaaagcat ttagccaaca
     9901 attatcatca tagccatatt gaagacgtat ttgcattgat aaaagaagcc aatgttaaac
     9961 gtacgttgat tacacatttg agcaatcgct ataatacaga agatataaat gaaatttatc
    10021 aaacattgat acaaaatgaa gatacaccta attttaattt tgtaaaagat tttgatagtt
    10081 ttaaaatcta atgacaaagt aataaacaat gcttgccaaa taacaataaa ttataaaaat
    10141 aacccgtcaa acacagtgct tgcgatgatg catacatgtg ttggcgggtt tgtttttatt
    10201 ggtcttctat attagaaagt tcaatactac ggtcgacggc agcgtttaga caatcttcga
    10261 aaatagatac taaatcatat tgtgacaatg tatcaaggcc agcttgtgtc gtaccacctt
    10321 tagaggtaat attttttctt aattgagcca tgctcaaatc tgaacgttca atcatcttac
    10381 ttgtacctat aataaggttg cgtatagatt cttcaacttg ttctttttct agaccaagtt
    10441 tcgtaccagc tttaacatat tgctcgaata catgatataa aaatgctggg ccgcttccgg
    10501 tgatagctgt tacttgatgt aaatgatctt ctgatacttc aattacagaa ccaaatgctt
    10561 taactaaatc gttaatttca tctttagatt tagggtcaaa gttgtttgaa aaactaatgc
    10621 cagtaacaga gtgtccaact tgcgcatttg tgtttggcat aattctagca actggatttt
    10681 ggcattctaa ttgttgttta atataatcaa tcggaatacc tgccataatt gaaatgaagc
    10741 aattgtcttt tgtaatatgt ggtttgatgc gtgttgctag agcatcaaaa tcatgtggtt
    10801 tggtacctaa aaatacataa tctgcatctt ttaataatgt cgcatcatca taactatagt
    10861 taacacctag tttttcagcg aatgctttta aagcttgttc attagattta tttgttaaat
    10921 atatatcatt ggcatctaag ttgcttgagt taataattcc tgtaaatata gcttgtgcca
    10981 tattaccagc tccataaaat acgagtttca ttaacgtcac atccctttag tttttgattt
    11041 gtatgttata ttttcttatt attgtatata tattaggaaa gataatcaac ttgtctcaat
    11101 tatgtaaagt tgataaattg cattctatat aattgaaaag gaaggttaat tgatgaaagg
    11161 acaacatttt atagtgactg gtggtacgag tggtttaggt ttatcgattg tccgaaagtt
    11221 actagaaaat aaagtgcatg taacgttgtt ggtgcgtgat gttgataaag caactagaat
    11281 ctttgaacag gaattgggga aaactataaa tgttattcct tgtgacttaa atgatttgaa
    11341 gtcgatacag gcactgcaat ttgaagacaa tacatcattc gatggattca tttatagtgc
    11401 aggtttaggt tatttcaaat ctatttcaga ccatagtttt tcagaaatga ttgaaacgta
    11461 tcagcttaat ttaattagtt ttaatgtttt atatactgtg ttgagaccat atttaacatc
    11521 taacgcacat attgtaggta tttcaagtca agctgcattt tccacacaag caaatgctgc
    11581 acattatggg gcatcgaaag cagggtttta tgcattaatg aatgctttaa gactagagtc
    11641 accaaactta catatcatga cagttaatgt tggcccgatt gatacacctt ttcatcaaaa
    11701 agccgaccca tcaatgaagt atgctaaaaa gatgggggaa attatgcttg atgctaatca
    11761 gcttgcagaa gatatcattt atggcattaa aacaaaacaa ttggaaatta atcggcctaa
    11821 atggatgcat catgcgttga aaatgtatca aattgcgcct agattttttg aacgatgttt
    11881 ccctaaatta tttaaaaata aagcataaat aggtgggatg gcaatgaact gtcaatcgga
    11941 gagtgtggta agactttgtg tacgatatgc ggaacaacta tcagtatttg aagaatttac
    12001 agtgctggat attttaggcg atatttctgt agaccaaatt tcagacggta cattatatta
    12061 tacttgtgaa aaattcaaac tacttgtgtt gcaaggaaat gtgctgggtg tacaaataat
    12121 aactaataat gatgaatcaa catgcgaagt gaaatataga aagatgtttt aataaaatta
    12181 ttaagagatg aagtggcaga agtttgctta aggcaatgcc gcaaggcgaa attactaaat
    12241 actgatgcgt gaggacaacg ctttaatttc tatcataagt gagtaatcat aatcataaat
    12301 tatagcgaat tgtacataaa caacagcagt aaggcatttt tataatgaaa cgcctcatgc
    12361 tgctgttttt attactgaaa atgacttcta cactaaatga ttggtatatt ccaaatcctt
    12421 tacacgagct cttgctgttt taatctgatc taaactaact ttagtatgat agttttcaat
    12481 attttctttt aattggtcga cgctacttgc acctacaatg atggaaccaa gcacgtcatg
    12541 tgatgttaaa taactaaatg tcaatgcaga taaattactt tcaatttctt taatagaggc
    12601 tattgtttca cccaattcat catgagaata atcaaaaata ccatctttaa atttattgtc
    12661 gagcacatta acactgtttg aagttaataa tcctttagac acaggtcctc ttgccaatac
    12721 tttaacatca ttgttgtgaa ttgcatctaa taatgattct ggacgattat caatcaaatt
    12781 gaattgagac attatcgttt cgatttgact atgttttaaa taataatcaa ttacatttgg
    12841 gcgaatagaa gaaataccgt aagcacgtat aattccttct tgtttcaatt catcaaatgc
    12901 gcttattgtt tcgtctaatg ggtcatcaat ggttccgcca tgaagttgat ataaatcgat
    12961 atgatcgata cctaaacgct ttagtgaacc tttaactgcc tctttaatat aggatttact
    13021 cggatcccat gttgtactgc catcttttgt taaacgattg cctacttttg taccaataaa
    13081 aatatcatcg cgttgttgat atttaagaag tgccttacca acaactgatt catttatacc
    13141 tttatcgtac atatctgctg tatcaaaata agtgatacca ttttcaacag cacaatctat
    13201 aatttgttcg gcttttttta aatctgtacc taaactcatg caacctagac ctaattcaga
    13261 taatgaaata ccacttttta atatattttt ttgcatgtct gttctccttt cgatacactt
    13321 tagctaacaa gcatagtgta tcaatatata atatgaattt aaaggaacgt gattataatg
    13381 gatttaaatg aaaaaacaat tgatcgaaca gttatttata atggcaaaat tgtagatgta
    13441 gaaattcata cagtgacatt accaaacggt gaaacgtcaa caagagaatt agtttatcat
    13501 aatggtgcag ttgctgtttg tgcagtgaca cctaaaaaag aagtcgtatt agtgaaacag
    13561 tatcgtaaac cagtagaaaa accattgcta gaaattccag ctggtaaatt agaagatgat
    13621 gaagatagag tcgaagcggc aaaacgtgaa ttagaagaag aaacaggata tattgccaaa
    13681 gagttaacac atgttgtaga tatgtatggt tcaccaggtt tttgcgatga acaattatca
    13741 atatatttta cggataattt agaagaaggt acagttcact tagatgaaga tgaatttgtc
    13801 gaagtcatta aagttccgat tgagaatgtt aaatcgatgc taatgaataa agaaatagaa
    13861 gatgcgaaga ctatcattgc attgcaacac ctattattaa attataatca ttctaaataa
    13921 caaatgagaa aagcttgcat tttattgaga aaactgttag ttttaattgt gaagtttgaa
    13981 ataatttgta atgattttaa ttattagtag gggagtggac atcgttggaa gaacgattaa
    14041 atcgcgttaa gcaacaatta caacaatcat catataagct aacgccacaa cgcgaagcta
    14101 ctgttagagt tctaattgaa aatgaaaaag atcatctaag tgctgaagac gtatatctga
    14161 aagtaaaaga taaagcgcct gaaattggct tggcgacagt atacagaacg ttagagttgt
    14221 tagctgaact aaaagttgtc gacaaaatta actttggtga tggcgtcgct cgttttgatt
    14281 taagaaaaga aggcgcaaaa catttccacc atcatttagt atgtatggaa tgtggtcgtg
    14341 tagatgaaat cgatgaagat ttgttaccag aagttgaaaa tcgagttgaa aatgagttca
    14401 attttaaaat tttagatcat cgtttaactt tccatggtgt gtgtgaaacg tgccaagcta
    14461 aaggtaaagg atagtaaatt gcgtaggtta aattaacctt cgcttttttt agaggtgtgg
    14521 ttatggaaac aattattgaa gaatatttgc gttttataca aattgaaaaa ggactaagtt
    14581 ctaacacaat tggtgcttat agacgtgatt tgaaaaagta tcaagattat atgactgaac
    14641 atcatatctc gcatattgat tttatagatc gacaattaat tcaagagtgt ttggggcatt
    14701 taatagacca agggcaatct gctaaatcta ttgcgcgatt tatttcaaca atccgtagtt
    14761 ttcatcaatt tgctataaga gaaaaatatg cggcgaaaga tccaacggta ttattagatt
    14821 caccaaaata tgacaaaaaa ttgcctgacg ttttaaatgt tgacgaagtg ttggctttat
    14881 tagaaacgcc agatttaaat aaaattaatg gatatcgtga tcgtacgatg ttagaacttc
    14941 tgtacgcaac gggaatgcgt gtatctgaat tgatacattt agagttagaa aacgtgaact
    15001 taataatggg atttgtacgc gtatttggta aaggcgataa agaaagaatt gtaccattag
    15061 gcgacgcagt cattgagtac ttaactactt atattgaaac gattagaccg caacttttaa
    15121 aaaagactgt tactgaagtc ttatttttaa atatgcatgg taaaccttta tcacgacaag
    15181 caatatggaa aatgattaaa caaaatggtg taaaggcaaa cattaaaaag acgttaacgc
    15241 cacatacgtt acgccactct tttgcgacac atttattgga aaatggcgca gatttaagag
    15301 cagtgcaaga gatgttaggt cactctgaca tatctactac ccaactctat acacatgttt
    15361 cgaaatctca aattagaaaa atgtataacc aatttcatcc tagagcataa agtgaacaat
    15421 aactcaaaag tcacaataca catgactaaa aatgtctgtg ctattgtggc ttttttaaat
    15481 tggttgatta attacgtcta tgttttctta attgaatcgc ttcttctttt gctgcaatca
    15541 cttctgaacg atcacggcgc atgtgatggt ctacaataaa aggatctgtt gctgtttcct
    15601 gattataatc atagtctgga tagttggcct tgatgatgcg ttcaaagact ggagttattg
    15661 gtaatataac agatgaaaaa ggctttgctg cattcaattt tgcaatctgt tgctcaatta
    15721 acaactgata atcatttaaa ttaaggtata acgcatctct atctttagca ttttgtatta
    15781 tttctttaga tttattaaaa gacttatagg cgccttttaa attattgcgg cgataatggt
    15841 aacaagcagt tgcaaacaag attaaactaa caactgcatc ttgcttactg tagttatttt
    15901 cagctttcca tgcatcttct aaaatgtcat gacataggaa ataatgttgc ttagtatgaa
    15961 attgataata gaaatttatc agtgcctgtt gcattttgtt atcaccccaa tttaaaagta
    16021 agttattttc atgctataat attttagaga attatgcaca tatgacgcaa tacgaggtag
    16081 atattatgta tgaagttaaa ttagatgctt tcaatggacc attagattta ttgctgcatc
    16141 ttatccaaaa atttgaaata gatatttatg atattcctat gcaagcatta acagagcagt
    16201 atatgcagta cgttcatgca atgaaacagc ttgaaattaa tattgcaagt gaatacctag
    16261 tattagcgtc agaactctta atgattaaaa gtaagatgct attaccacaa tcaacatcag
    16321 atatggatgt tgatgatgac ccacgggaag atttagttgg gcgtttaata gaatatcaaa
    16381 attataaaga atatactgct attttaaatg acatgaaaga agaaagagat ttttatttta
    16441 caaaaagacc gacagattta tctcatttgg aaacagatga atcttgggat ccaaatcata
    16501 cgattgattt aactgaatta attgtagctt atcaaagagt taaaaataga gttgagttaa
    16561 atacacctaa atctgttgaa atccgaaaag agacatttac cattcaacaa gctacagaac
    16621 aagtgacatc gagattgaaa gataaagatc attttaactt ctttagtctg tttacgtttt
    16681 ctgagccaat tgaacaagta gtcactcact ttttagctat tttagagatg tcaaaagcag
    16741 gaataattaa tattgagcag caacgtaatt ttgaagatat taacattatt agaggagtga
    16801 actaccattt tggataatca tggtatatta gagtcgcttt tatttacagc tggcgatgaa
    16861 ggtttagatg aaaaacaact attagaaata ttagatatgt cgaaagacca actcgttgaa
    16921 ttaattgaaa attattcatc acatggatta atgatacaac gatttggaat gacgtatgtt
    16981 ttaacgacta aaaaagaagc ggcaacgtat attgaacaat taattgaaca aaagtcacaa
    17041 atgaaattat cacaagcagc aatggaagta ctatcaatta ttgcttataa ccagccatta
    17101 tcaagaagtg atattgaatt aattcgtagt atcaattcag atggtgcagt taagacattg
    17161 attgccaaag gactagttga ggctaaagtg gttaatgaac agcgtagcca acagttaatt
    17221 actactgatt tatttttaaa tgtatttggt atttccaata tagaagattt gccgacaact
    17281 gaagaagacg atgaagaaat ggatgctttt ttcagtaatc tagtcaatca aaaaggagaa
    17341 aataatgact aaagaattag aacgattaca aaaacgtata gctaatagcg gttatacttc
    17401 aagacgtaag gcagaaactt taatttctga aggaaaagtg aaagtgaatg gtacgactgt
    17461 cactgaatta ggaacaaaag tgaagccatc agatacgatt gaagttgaag gtattaaaat
    17521 agaactagaa gacaaaattt acattttatt ccataaacca actcaagtga taacaagtgt
    17581 atctgatgat agaggacgta cagtggttac agattacttt aaagatatag aagcacgtat
    17641 ttatcctgtt ggtcgtttag actatgatac ttctggattg ttattactca caaatgatgg
    17701 tgaatttact aatttaatga cacatccaag atatcaaatt aagaaaaaat atgttgcgaa
    17761 attaaaaggt tatttaatga gagaagaagt gaaagcgcta gaaaaaggta ttgaattaga
    17821 agatggtatg acgcaaccgg ctgaagtgaa agttaagaaa caagataaag ataaaaacac
    17881 aacattggtt gaaattacaa ttacagaagg tcgtaatcgc caagtcagaa gaatgttcga
    17941 acattttggt catcaagtga cgaaactgtc tcgtattgaa tatgggcctt taaatgttgt
    18001 cggtttgaat gctggtgaag gacgtgtatt gacgcctcat gaagtaaaag tgatgcgtca
    18061 tttagcagaa catgggaaat aattaaataa aatatgtatt tatcacaaag tttgagaatt
    18121 ttttcacaaa attttaggtt tttaataact gtatgcgctt tcctgtgata taatactttt
    18181 gtaaagaaaa gcatgtgtgg gaggtatgac ctgtatgtcg aacgaaatac ttatcgtaga
    18241 tgatgaggat agaatcagaa gattacttaa aatgtattta gaaagagaat cttttgaaat
    18301 ccatgaagca agtaatggcc aagaggctta tgaacttgca atggagaata attatgcttg
    18361 catactacta gatttaatgt tgcctgaaat ggatggtatc caggtggcaa ctaaattgcg
    18421 tgaacataaa caaacaccga ttattatgtt gactgctaaa ggtgaagaaa caaaccgtgt
    18481 tgaaggtttt gaatctggtg cagatgatta tatcgtcaaa ccattttcac caagagaagt
    18541 agtcttaaga gttaaagcac ttctaagaag aacgcaatct acaactgtag aacaaagcga
    18601 acctcacgca cgtgatgtga ttgaatttaa acatttagaa atagataatg atgcacatcg
    18661 cgtacttgct gataatcaag aagttaattt gactcctaaa gagtacgaat tattaatata
    18721 tttagctaaa acaccaaata aagtatttga ccgtgaacaa ttattaaaag aagtttggca
    18781 ttatgaattc tatggtgatt taagaacagt tgatactcat gttaaacgac ttagagaaaa
    18841 gttaaatcgt gtgtctagtg aagctgcgca tatgattcaa acagtctggg gcgttgggta
    18901 taaatttgag gttaaatcta atgatgagcc ggctaaatag tgtcgtaatt aaactgtggt
    18961 taactattat tttaatagtg acgacagttt taattttatt aagtattgct ttaattacct
    19021 ttatgcaata ctatttcaca caagaaaccg aaaatgccat aagagaagat gctagacgta
    19081 taagttcact ggtcgaacaa tcacataata aagaagaagc aataaaatat agtcaaacat
    19141 taattgaaaa tcctggtggg ttgatgatta taaataataa acatcgtcaa tcaacggctt
    19201 cactttctaa tattaaaaag caaatgttga atgaagtagt caacaacgac cattttgacg
    19261 atgtgtttga taaaggtaaa tctgttactc gaaatgtaac gattaaagaa aagggctcat
    19321 ctcaaacata tattttgtta ggctatccaa caaaagcaca gaagaatagt catagcaaat
    19381 atagtggagt ctttatatat aaagacttga aatcaatcga agatacaaat aatgctatta
    19441 cgattatcac cataattacg gctgttattt tcttaacaat tacaacagtc tttgcgtttt
    19501 tcttatcgtc aagaattaca aaacctttaa gacgtttaag agaccaagct acacgtgtat
    19561 ctgaagggga ttactcttat aaaccttctg tcacaacgaa agatgaaatt ggtcaattat
    19621 cgcaggcatt taatcagatg agtacagaaa tcgaagagca tgtcgacgca ttatccacat
    19681 ctaaaaatat tagagacagc ttaattaact ctatggtaga aggtgtccta ggtattaatg
    19741 agagtcgaca aattatctta tctaataaga tggcgaatga tattatggac aatattgatg
    19801 aagatgctaa agctttctta ttaagacaaa tagaagatac ttttaaatca aaacaaactg
    19861 aaatgcgcga tttagaaatg aatgcacgat tctttgttgt gaccacaagc tatatcgaca
    19921 agattgaaca gggaggtaaa agtggtgttg ttgtgacagt tcgtgatatg actaatgagc
    19981 acaatctaga tcaaatgaag aaagatttca ttgctaatgt atcacatgaa ttacgtacac
    20041 cgatatcatt acttcaaggt tatactgaat caattgtaga tggtattgtt acagaaccgg
    20101 atgaaataaa agaatcgctt gccattgtcc ttgatgaatc gaaacgttta aatcgtttag
    20161 ttaatgaatt gttaaatgtc gcacgcatgg atgctgaagg gttatccgta aataaagaag
    20221 ttcagcctat tgcagcgtta ctagataaga tgaaaattaa gtatcgccaa caagctgatg
    20281 atttaggtct aaatatgact tttaattatt gtaagaagcg tgtttggagt tatgatatgg
    20341 atcgcatgga ccaagtacta acgaacttaa ttgataatgc atcacgttat acgaaacctg
    20401 gagatgaaat tgcaattact tgtgatgaaa atgaaagcga agatatttta tacattaaag
    20461 atacaggtac aggcattgca ccagaacatt tacaacaagt atttgatcgt ttttataaag
    20521 ttgatgcagc gagaacgcga ggtaaacaag gtaccggttt aggtttgttc atttgtaaaa
    20581 tgattatcga agagcatggt ggttccatag atgttaaaag cgaattaggg aaaggcacaa
    20641 catttattat taaactacca aaaccagaat aaaattgaat atagttattt cagaacgcat
    20701 gttattgatt agagactcta atttatagca tgcgtttttt gattgatgtg gggaattttg
    20761 tatgtggatt agaacttagg gtttttgcga atatcaacta ttaaatatat tactaattta
    20821 tacaaaaata taaagtttga taaagttatt tatttgatta taaaaatagg gtaaaatata
    20881 gatatattgt attaattaaa ttattcgagg tgtcatatga aaaaattcat tggatcagtt
    20941 ttagctacga cattaatttt agggggatgt tccacgatgg aaaatgaatc aaaaaaagac
    21001 acaaaaacag aaacaaaatc tgtaccagaa gaaatggaag cttcaaaata tgtaggccaa
    21061 ggctttcaac cgcctgcaga aaaaaatgcg attgaatttg cgaagaagca tcgtaaagaa
    21121 tttgaaaaag taggtgaaca attctttaaa gataactttg gactaaaagt taaagctaca
    21181 aatgttgtag gtaaagatga tggtgtagaa gtttatgtgc attgtgaaga tcatggcatt
    21241 gtatttaatg caagtctacc tttgtacaaa gatgccatcc atcaaaaagg atcaatgcgc
    21301 agtaatgaca atggtgatga tatgagtatg atggtgggta cagtgctgag tggctttgaa
    21361 tatcgagcgc aaaaagaaaa gtatgataac ttatataaat tcttcaaaga aaatgaaaag
    21421 aaatatcaat atacaggctt tacaaaagag gcaattaaca agacacaaaa tgtcggatat
    21481 aaaaatgaat atttttatat tacatactct tctagaagtt taaaagaata tcgaaagtat
    21541 tatgaaccac tgattcgaaa aaatgataaa gaatttaaag aaggaatgga acgagcaaga
    21601 aaagaagtga attacgctgc aaatacagat gctgttgcta cacttttttc tactaagaaa
    21661 aactttacta aagacaatac agtagatgat gtaatcgaac taagtgataa attatataat
    21721 ttaaaaaata aaccagataa atctacaatc acaatacaaa tagggaaacc cactattaat
    21781 actaagaaag ccttttatga tgataatcgt ccaatagaat atggggtgca cagtaaagat
    21841 gaataaaatt aatgataggg atttaacaga attaagtagt tactgggttt atcaaaatat
    21901 tgatataaaa aaagaattta aagttaatgg aaaaaggttt aaacaagtag acagttataa
    21961 tgatgataag aatagtaatt tgaatggtgc tgctgatatt aaaatatatg agttattaga
    22021 tgataaaagt aaaccaactg gtcaacagac aataatttat caaggaacat ctaatgaggc
    22081 aattaatcca aataatccat taaaatcatc ggggtttgga gatgattggc tccaaaatgc
    22141 taaattaatg aataatgata atgaaagcac agattattta aagcaaacag atcaattatc
    22201 aaatcaatat aaaataaagt tagaagatgc agatagatta tcaaatagtg attttttaaa
    22261 aaaatataga atggaatcaa gtaacttcaa aaacaaaacc attgtggcgg atggcggtaa
    22321 ttcggaaggc ggtgcaggag caaaatatca aggagcgaaa catccgaatg aaaaagttgt
    22381 tgctactgac tcagcaatga ttccttatgc tgcttggcag aaatttgcta gaccacgctt
    22441 tgataatatg attagtttta atagtaccaa cgatttatta acatggttac aagatccatt
    22501 catcaaagat atgccaggaa aacgcgttaa cattaatgat ggtgtgccca ggttagatac
    22561 tttaatagac agccatgtag gttataaaag gaagttaaat agaaaagata acacatacga
    22621 tactgtacca ctaatcaaaa taaagtcggt aaaagataca gaaattaaaa atggaaaaaa
    22681 gtaaaaaaga ctattaacat aacattagat atggatgggc gaattccaat aaatgtttgg
    22741 acaggagatt cgattgcacg ttctggaaga ggaactttaa ttaaacttaa tttagaaaat
    22801 cttgatgcgt tgagtaaact gattactggt gaaacaagtg gtatgttagc agaatgcgta
    22861 atctttttaa atgaaagttt taacatctca gaaaatgaaa ataaaaattt tgcagataga
    22921 aagaaacaat tatcagaagg atttaaggat aagattaact tatttcagtt agaagaaatg
    22981 gaaagaactt taattagtaa aataaactca cttgaagaag ttgcagatga aacaatagaa
    23041 agtattagtg ctgttaaaca cttattacct gattttgcat tggatgcatt aaaagaaaga
    23101 attaatgagt tgtttaaagg tataaaatct tttatagaaa aagtgtatga tagtatagat
    23161 aatgaaattt tagaaatttt caaaaatata gatcacgact tcagagatgg agtatctgaa
    23221 gaaatgatga aacatttgaa agtagtgaaa cagaatatag accaaataaa aaatcaaaat
    23281 gatatttatg gtaggcaaat tgcagatatt agaagtatta tgaaacaaca agatgcaaca
    23341 attttagatg gaaattttca aattaattgt agcggcgaaa atatggtaca gggtctagtt
    23401 ataccttcta attatttagg aagaaaaatg aaaatattaa aagaccatat cgatgatggt
    23461 attaaaaaaa tagcagacta tgttcaaggt atatatgatg aatatgcatc gaaaattgtc
    23521 gatgtaataa aatatttgat taatacaatt cccaaaatac gtaagaattt aagacatgca
    23581 attgaaatgt taaatgtaaa aaagaaagaa tttttgtccc tgattcctaa tgtaacttgt
    23641 aattatatta aaactaaatt agaagaatta gataatactt taggcaaatg ggagcctttt
    23701 cttaatgatt taaaagcagt gtcaccaatt ttagataacc atttagatga tattgttaag
    23761 aacatgaagc ctttgattgt acaaatgata tttgaaccat cacattatga tgatatgttt
    23821 atttcaagaa aagctttaac gccagtgttc tcaagcgttt tataaagctt gtaaaaaata
    23881 taagggcaaa aaaagggcgg atttaagcta acttggaatg ttttcgagtt tttgagttag
    23941 ttctctatcc attttttcag ttacatgagt atatatgcga atggttgttt tttcatctac
    24001 atgtcctacc cttttcataa ttgcttttaa agaaacattc atttctacta ataaagttat
    24061 gtgtgtatgt ctaaatgtgt gcgtggtaac tttcttattc atatttaaag cttttgtagt
    24121 tttcttaagc acaccggcga tttgattatt acataaagga ttcccttttt ttgttgtgaa
    24181 tatgaaccct ctgtcaacat agctcgaatt ccatcttttc aacattttgt tttccagtat
    24241 tatcttttta aaaatttcta cggttctaga attgatgctg atacttcttt ttgaacttat
    24301 agtctttgta gtgtctttgt atccgaatcc ttcctcgtat ttaatgcggt gaattgtacc
    24361 tgttatattg atagttttgt ttaataaatc tatatctttt tcctgcagtg cttgtagttc
    24421 tcctatgcgc ataccagtta aagcctgtac ttctaagatg ctggcaatta aaatgcgatt
    24481 tcgcttgtgt aacttattat catttagtat atgatcacgt atctgtagga cttggttcat
    24541 ttctaaatag ttgtacattt tagattcatc tttttcgata tcctctattg tttttcttct
    24601 tttaggaatt ttgacattag ttaacaaata ttcatttgga taattgtaaa atttaactgc
    24661 atatttaata gctcctttca tatctccgag ttgacgggtt acttgatttt gagaatagat
    24721 atctgataat ttattaataa atatctgcat atattttgta tctagtttgt ttaaaagcaa
    24781 gttctcagag ctgtatcgtt taatgtttct aattcttatt tttatattat taagagtagt
    24841 caactttgaa cctgatgttt ttatatgata ttcaagccat tcatctaata gcgcgtgaaa
    24901 agtcaaagtt tttaattcgc ttgacgactt gttgttcagt ttttctttta ttttttcttc
    24961 taaacgaaac attgcttctt tttgagattg ttttgtattc ttgttcaaca caacacttac
    25021 gcgcttccat ttatctgtgt atggatcttt atacttctcg tagtatctgt atttagtttc
    25081 gttatttttg tttttaaatt tttcaatcca catgtttata cctcctgcaa gaacgtatgt
    25141 tctataaaat attaaaaaat aataagggta gtcgggctac ccgtaattta gtactaggta
    25201 ctaaatgtgt tataataaaa taaaaagtag gtgataagat gactcaattt ctaggggcgc
    25261 ttcttcttac aggagtttta ggttacatac catataaata tctaacaatg ataggtttag
    25321 ttagtgaaaa aaacaaggtt atcaatactc ctgtattatt g
//
LOCUS       DALAGD010000035        25026 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.035, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000035 DALAGD010000000
VERSION     DALAGD010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 25026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25026
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.035"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(51..404)
                     /gene="rnpA"
                     /locus_tag="O2701_001164"
     CDS             complement(51..404)
                     /gene="rnpA"
                     /locus_tag="O2701_001164"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCZ4293067.1"
                     /translation="MLLEKAYRIKKNADFQRIYKKGHSVANRQFVVYTCNNKEIDHFR
                     LGISVSKKLGNAVLRNKIKRAIRENFKVHKSHILAKDIIVIARQPAKDMTTLQIQNSL
                     EHVLKIAKVFNKKIK"
     gene            complement(526..663)
                     /gene="rpmH"
                     /locus_tag="O2701_001165"
     CDS             complement(526..663)
                     /gene="rpmH"
                     /locus_tag="O2701_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCZ4293068.1"
                     /translation="MVKRTYQPNKRKHSKVHGFRKRMSTKNGRKVLARRRRKGRKVLS
                     A"
     gene            1394..2755
                     /gene="dnaA"
                     /locus_tag="O2701_001166"
     CDS             1394..2755
                     /gene="dnaA"
                     /locus_tag="O2701_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCZ4293069.1"
                     /translation="MSEKEIWEKVLEIAQEKLSAVSYSTFLKDTELYTIKDGEAIVLS
                     SIPFNANWLNQQYAEIIQAILFDVVGYEVKPHFITTEELANYSNNETATPKETTKPST
                     ETTEDNHVLGREQFNAHNTFDTFVIGPGNRFPHAASLAVAEAPAKAYNPLFIYGGVGL
                     GKTHLMHAIGHHVLDNNPDAKVIYTSSEKFTNEFIKSIRDNEGEAFRERYRNIDVLLI
                     DDIQFIQNKVQTQEEFFYTFNELHQNNKQIVISSDRPPKEIAQLEDRLRSRFEWGLIV
                     DITPPDYETRMAILQKKIEEEKLDIPPEALNYIANQIQSNIRELEGALTRLLAYSQLL
                     GKPITTELTAEALKDIIQAPKSKKITIQDIQKIVGQYYNVRIEDFSAKKRTKSIAYPR
                     QIAMYLSRELTDFSLPKIGEEFGGRDHTTVIHAHEKISKDLKEDPIFKQEVENLEKEI
                     RNV"
     gene            3033..4166
                     /gene="dnaN"
                     /locus_tag="O2701_001167"
     CDS             3033..4166
                     /gene="dnaN"
                     /locus_tag="O2701_001167"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCZ4293070.1"
                     /translation="MMEFTIKRDYFITQLNDTLKAISPRTTLPILTGIKIDAKEHEVI
                     LTGSDSEISIEITIPKTVDGEDIVNISETGSVVLPGRFFVDIIKKLPGKDVKLSTNEQ
                     FQTLITSGHSEFNLSGLDPDQYPLLPQVSRDDAIQLSVKVLKNVIAQTNFAVSTSETR
                     PVLTGVNWLIQENELICTATDSHRLAVRKLQLEDVSENKNVIIPGKALAELNKIMSDN
                     EEDIDIFFASNQVLFKVGNVNFISRLLEGHYPDTTRLFPENYEIKLSIDNGEFYHAID
                     RASLLAREGGNNVIKLSTGDDVVELSSTSPEIGTVKEEVDANDVEGGSLKISFNSKYM
                     MDALKAIDNDEVEVEFFGTMKPFILKPKGDDSVTQLILPIRTY"
     gene            4547..4792
                     /gene="yaaA"
                     /locus_tag="O2701_001168"
     CDS             4547..4792
                     /gene="yaaA"
                     /locus_tag="O2701_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HCZ4293071.1"
                     /translation="MIILVQEVVVEGDINLGQFLKTEGIIESGGQAKWFLQDVEVLIN
                     GVRETRRGKKLEHQDRIDIPELPEDAGSFLIIHQGEQ"
     gene            4789..5901
                     /gene="recF"
                     /locus_tag="O2701_001169"
     CDS             4789..5901
                     /gene="recF"
                     /locus_tag="O2701_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCZ4293072.1"
                     /translation="MKLNTLQLENYRNYDEVTLKCHPDVNILIGENAQGKTNLLESIY
                     TLALAKSHRTSNDKELIRFNADYAKIEGELSYRHGTMPLTMFITKKGKQVKVNHLEQS
                     RLTQYIGHLNVVLFAPEDLNIVKGSPQIRRRFIDMELGQISAVYLNDLAQYQRILKQK
                     NNYLKQLQLGQKKDLTMLEVLNQQFAEYAMKVTDKRAHFIQELESLAKPIHAGITNDK
                     EALSLNYLPSLKFDYAQNEAARLEEIMSILSDNMQREKERGISLFGPHRDDISFDVNG
                     MDAQTYGSQGQQRTTALSIKLAEIELMNIEVGEYPILLLDDVLSELDDSRQTHLLSTI
                     QHKVQTFVTTTSVDGIDHEIMNNAKLYRINQGEIIK"
     gene            5911..7845
                     /gene="gyrB"
                     /locus_tag="O2701_001170"
     CDS             5911..7845
                     /gene="gyrB"
                     /locus_tag="O2701_001170"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCZ4293073.1"
                     /translation="MVTALSDVNNTDNYGAGQIQVLEGLEAVRKRPGMYIGSTSERGL
                     HHLVWEIVDNSIDEALAGYANQIEVVIEKDNWIKVTDNGRGIPVDIQEKMGRPAVEVI
                     LTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSQDLEVYVHRNETIYHQAYKKGVPQF
                     DLKEVGTTDKTGTVIRFKADGEIFTETTVYNYETLQQRIRELAFLNKGIQITLRDERD
                     EENVREDSYHYEGGIKSYVELLNENKEPIHDEPIYIHQSKDDIEVEIAIQYNSGYATN
                     LLTYANNIHTYEGGTHEDGFKRALTRVLNSYGLSSKIMKEEKDRLSGEDTREGMTAII
                     SIKHGDPQFEGQTKTKLGNSEVRQVVDKLFSEHFERFLYENPQVARTVVEKGIMAARA
                     RVAAKKAREVTRRKSALDVASLPGKLADCSSKSPEECEIFLVEGDSAGGSTKSGRDSR
                     TQAILPLRGKILNVEKARLDRILNNNEIRQMITAFGTGIGGDFDLAKARYHKIVIMTD
                     ADVDGAHIRTLLLTFFYRFMRPLIEAGYVYIAQPPLYKLTQGKQKYYVYNDRELDKLK
                     SELNPTPKWSIARYKGLGEMNADQLWETTMNPEHRALLQVKLEDAIEADQTFEMLMGD
                     VVENRRQFIEDNAVYANLDF"
     gene            7882..10545
                     /gene="gyrA"
                     /locus_tag="O2701_001171"
     CDS             7882..10545
                     /gene="gyrA"
                     /locus_tag="O2701_001171"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HCZ4293074.1"
                     /translation="MAELPQSRINERNITSEMRESFLDYAMSVIVARALPDVRDGLKP
                     VHRRILYGLNEQGMTPDKSYKKSARIVGDVMGKYHPHGDLSIYEAMVRMAQDFSYRYP
                     LVDGQGNFGSMDGDGAAAMRYTEARMTKITLELLRDINKDTIDFIDNYDGNEREPSVL
                     PARFPNLLANGASGIAVGMATNIPPHNLTELINGVLSLSKNPDISIAELMEDIEGPDF
                     PTAGLILGKSGIRRAYETGRGSIQMRSRAVIEERGGGRQRIVVTEIPFQVNKARMIEK
                     IAELVRDKKIDGITDLRDETSLRTGVRVVIDVRKDANASVILNNLYKQTPLQTSFGVN
                     MIALVNGRPKLINLKEALVHYLEHQKTVVRRRTQYNLRKAKDRAHILEGLRIALDHID
                     EIISTIRESDTDKVAMESLQQRFKLSEKQAQAILDMRLRRLTGLERDKIEAEYNELLN
                     YISELEAILADEEVLLQLVRDELTEIRDRFGDDRRTEIQLGGFENLEDEDLIPEEQIV
                     ITLSHNNYIKRLPVSTYRAQNRGGRGVQGMNTLEEDFVSQLVTLSTHDHVLFFTNKGR
                     VYKLKGYEVPELSRQSKGIPVVNAIELENDEVISTMIAVKDLESEDNFLVFATKRGVV
                     KRSALSNFSRINRNGKIAISFREDDELIAVRLTSGQEDILIGTSHASLIRFPESTLRP
                     LGRTATGVKGITLREGDEVVGLDVAHANSVDEVLVVTENGYGKRTPVNDYRLSNRGGK
                     GIKTATITERNGNVVCITTVTGEEDLMIVTNAGVIIRLDVADISQNGRAAQGVRLIRL
                     GDDQFVSTVAKVKEDAEDETNEDEQSTSTVSEDGTEQQREAVVNDETPGNAIHTEVID
                     SEENDEDGRIEVRQDFMDRVEEDIQQSSDEE"
     gene            complement(10632..11444)
                     /locus_tag="O2701_001172"
     CDS             complement(10632..11444)
                     /locus_tag="O2701_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HCZ4293075.1"
                     /translation="METLNSINIPKRKEDSHKGDYGKILLIGGSANLGGAIMLAARAC
                     VFSGSGLITVATHPTNHSALHSRCPEAMVIDINDTKMLTKMIEMTDSILIGPGLGVDF
                     KGNNAITFLLQNIQPHQNLIVDGDAITIFSKLKPQLPTCRVIFTPHLKEWERLSGIPI
                     EEQTYERNREAVDRLGATVVLKKHGTEIFFKDEDFKLTIGSPAMATGGMGDTLAGMIT
                     SFVGQFDNLKEAVMSATYTHSFIGENLAKDMYVVPPSRLINEIPYAMKQLES"
     gene            11770..13284
                     /gene="hutH"
                     /locus_tag="O2701_001173"
     CDS             11770..13284
                     /gene="hutH"
                     /locus_tag="O2701_001173"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HCZ4293076.1"
                     /translation="MTLYLDGETLTIEDIKSFLQQQSKIEIIDDALERVKKSRAVVER
                     IIENEETVYGITTGFGLFSDVRIDPTQYNELQVNLIRSHACGLGEPFSKEVALVMMIL
                     RLNTLLKGHSGATLELVRQLQFFINERIIPIIPQQGSLGASGDLAPLSHLALALIGEG
                     KVLYRGEEKDSDDVLRELNRQPLNLQAKEGLALINGTQAMTAQGVISYIEAEDLGYQS
                     EWIAALTHQSLNGIIDAYRHDVHAVRNFQEQINVAARMRDWLEGSTLTTRQSEIRVQD
                     AYTLRCIPQIHGASFQVFNYVKQQLEFEMNAANDNPLIFEEANETFVISGGNFHGQPI
                     AFALDHLKLGVSELANVSERRLERLVNPQLNGDLPAFLSPEPGLQSGAMIMQYAAASL
                     VSENKTLAHPASVDSITSSANQEDHVSMGTTAARHGYQIIENARRVLAIECVIALQAA
                     ELKGVEGLSPKTRRKYDEFRSIVPSITHDRQFHKDIEAVAQYLKQSIYQTTACH"
     misc_binding    13357..13573
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13663..14949
                     /gene="serS"
                     /locus_tag="O2701_001174"
     CDS             13663..14949
                     /gene="serS"
                     /locus_tag="O2701_001174"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCZ4293077.1"
                     /translation="MLDIRLFRNEPDTVKSKIELRGDDPKVVDEILELDEQRRKLISA
                     TEEMKARRNKVSEEIALKKRNKENADDVIAEMRTLGDDIKEKDSQLNEIDNKMTGILC
                     RIPNLISDDVPQGESDEDNVEVKKWGTPREFSFEPKAHWDIVEELKMADFDRAAKVSG
                     ARFVYLTNEGAQLERALMNYMITKHTTQHGYTEMMVPQLVNADTMYGTGQLPKFEEDL
                     FKVEKEGLYTIPTAEVPLTNFYRNEIIQPGVLPEKFTGQSACFRSEAGSAGRDTRGLI
                     RLHQFDKVEMVRFEQPEDSWNALEEMTTNAEAILEELGLPYRRVILCTGDIGFSASKT
                     YDLEVWLPSYNDYKEISSCSNCTDFQARRANIRFKRDKAAKPELAHTLNGSGLAVGRT
                     FAAIVENYQNEDGTVTIPEALVPFMGGKTQISKPVK"
     gene            15599..16294
                     /locus_tag="O2701_001175"
     CDS             15599..16294
                     /locus_tag="O2701_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCZ4293078.1"
                     /translation="MTTHLSFRQGVQECIPTLLGYAGVGISFGIVASSQNFSILEIVL
                     LCLVIYAGAAQFIMCALFIAGTPISAIVLTVFIVNSRMFLLSMSLAPNFKTYGFWNRV
                     GLGSLVTDETFGVAITPYLKGEAINDRWMHGLNITAYLFWAISCVAGALFGEYISNPQ
                     TLGLDFAITAMFIFLAIAQFESITKSRLRIYIVLIIAVIVMMLSLSMFMPSYLAILIA
                     ATISAALGVMMEQ"
     gene            16291..16620
                     /locus_tag="O2701_001176"
     CDS             16291..16620
                     /locus_tag="O2701_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCZ4293079.1"
                     /translation="MITHMNMLILILLCGIVTLLIRIIPFIMISKVQLPDVVVRWLSF
                     IPITLFTALVIDSIIQQTPHGEGYTLNIPYIIALIPTVILSIITRSLTITIISGIVIM
                     AALRFFF"
     regulatory      16825..16923
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            16983..17951
                     /locus_tag="O2701_001177"
     CDS             16983..17951
                     /locus_tag="O2701_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCZ4293080.1"
                     /translation="MTNYTVDTLNLGEFITESGEVIDNLRLRYEHVGYHGQPLVVVCH
                     ALTGNHLTYGTDDYPGWWREIIDGGYIPIHDYQFLTFDVIGSPFGSSSPLNDPHFPKK
                     LTLRDIVRANERGIQALGYDKINILIGGSLGGMQAMELLYNQQFEVDKAIILAATSRT
                     SSYSRAFNEIARQAIHLGGKEGLSIARQLGFLTYRSSKSYDERFTPDEVVAYQQHQGN
                     KFKEHFDLNCYLTLLDVLDSHNIDRGRTDVTHVFKNLETKVLTMGFIDDLLYPDDQVR
                     ALGERFKYHRHFFVPDNVGHDGFLLNFSTWAPNLYHFLNLKHFKRK"
     gene            18242..19180
                     /locus_tag="O2701_001178"
     CDS             18242..19180
                     /locus_tag="O2701_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HCZ4293081.1"
                     /translation="MGVAYVFSKIQPKATIIATIALVFVALALYLVPGLGLIFALFAT
                     IPGIVLWNKSIQSFGISALITVIITTVLGNTFVLSAIILVLIASLIIGQLLKERTSKE
                     RILYVTTVAMSLISLIAFMLLQTFGRIPPSASIVKPFKQTLHEAITMSGADANMTQIL
                     EEGFRQATVQLPGFIIIITFLIVLINLIVTFPILRKFKIATPVFKPLFAWQMSGILLW
                     IYIIVIICLLFTGQPSVFQSILLNFQLVLSLVMYIQGLSVIHFFGKAKGLPNAVTILL
                     LVIGTILTPTTHIVGLLGVIDLSLNLKRIMKNNSKK"
     gene            19195..21162
                     /gene="gdpP"
                     /locus_tag="O2701_001179"
     CDS             19195..21162
                     /gene="gdpP"
                     /locus_tag="O2701_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP phosphodiesterase GdpP"
                     /protein_id="HCZ4293082.1"
                     /translation="MNRQSTKKALLIPFVIMIITAIVLMGVWFIFNSLIALIASIVLV
                     VMIIVSIILFRQALMKMDSYVDGLSAQISTTNNKAIKHLPIGIIVLDENDHIEWVNQF
                     MTDHMEANVISESVNEVFPNILKQLDRVKSVEIEYNQYHFQVRYSENDHCLYFFDITE
                     QVQTNELYENSKPIIATLFLDNYDEITQNMNDTQRSEINSMVTRVISRWATEYNIFFK
                     RYSSDQFVAYLNQKILADLEESKFDILSQLREKSVGYRAQLTLSIGVGEGTENLIDLG
                     ELSQSGLDLALGRGGDQVAIKSINGNVRFYGGKTDPMEKRTRVRARVISHALKDILAE
                     GDKVIIMGHKRPDLDAIGAAIGVSRFAMMNNLEAYIVLNETDIDPTLRRVMNEIDKKP
                     ELRERFITSDDAWDMMTSKTTVVIVDTHKPELVLDENVLNKANRKVVIDHHRRGESFI
                     SNPLLIYMEPYASSTAELVTELLEYQPTEQRLTRLESTVMYAGIIVDTRNFTLRTGSR
                     TFDAASYLRAHGADTILTQHFLKDDVDTYINRSELIRTVKVEDNGIAIAHGSDDKIYH
                     PVTVAQAADELLSLEGIEASYVVARREDNLIGISARSLGSVNVQLTMEALGGGGHLTN
                     AATQLKGVTVEEAIAQLQQAITEQLSRSEDA"
     gene            21159..21605
                     /gene="rplI"
                     /locus_tag="O2701_001180"
     CDS             21159..21605
                     /gene="rplI"
                     /locus_tag="O2701_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCZ4293083.1"
                     /translation="MKVIFTQDVKGKGKKGEVKEVPVGYANNFLLKKNYAVEATPGNL
                     KQLELQKKRAKQERQQEIEDAKALKETLSNIEVEVSAKTGEGGKLFGSVSTKQIAEAL
                     KAQHDIKIDKRKMDLPNGIHSLGYTNVPVKLDKEVEGTIRVHTVEQ"
     gene            21637..23037
                     /gene="dnaB"
                     /locus_tag="O2701_001181"
     CDS             21637..23037
                     /gene="dnaB"
                     /locus_tag="O2701_001181"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCZ4293084.1"
                     /translation="MDRMYEQNQMPHNNEAEQSVLGSIIIDPELINTTQEVLLPESFY
                     RGAHQHIFRAMMHLNEDNKEIDVVTLMDQLSTEGTLNEAGGPQYLAELSTNVPTTRNV
                     QYYTDIVSKHALKRRLIQTADSIANDGYNDELELDAILSDAERRILELSSSRESDGFK
                     DIRDVLGQVYETAEELDQNSGQTPGIPTGYRDLDQMTAGFNRNDLIILAARPSVGKTA
                     FALNIAQKVATHEDMYTVGIFSLEMGADQLATRMICSSGNVDSNRLRTGTMTEEDWSR
                     FTIAVGKLSRTKIFIDDTPGIRINDLRSKCRRLKQEHGLDMIVIDYLQLIQGSGSRAS
                     DNRQQEVSEISRTLKALARELECPVIALSQLSRGVEQRQDKRPMMSDIRESGSIEQDA
                     DIVAFLYRDDYYNRGGDEDDDDDGGFEPQTNDENGEIEIIIAKQRNGPTGTVKLHFMK
                     QYNKFTDIDYAHADMM"
     gene            23315..24598
                     /locus_tag="O2701_001182"
     CDS             23315..24598
                     /locus_tag="O2701_001182"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCZ4293085.1"
                     /translation="MSSIVVVGTQWGDEGKGKITDFLAEQSDVIARFSGGNNAGHTIQ
                     FGGETYKLHLVPSGIFYKDKLAVIGNGVVVDPVALLKELDGLNERGIPTSNLRISNRA
                     QVILPYHLAQDEYEERLRGDNKIGTTKKGIGPAYVDKVQRIGIRMADLLEKETFERLL
                     KSNIEYKQAYFKGMFNETCPSFDDIFEEYYAAGQRLKEFVTDTSKILDDAFVADEKVL
                     FEGAQGVMLDIDHGTYPFVTSSNPIAGNVTVGTGVGPTFVSKVIGVCKAYTSRVGDGP
                     FPTELFDEDGHHIREVGREYGTTTGRPRRVGWFDSVVLRHSRRVSGITDLSINSIDVL
                     TGLDTVKICTAYELDGKEITEYPANLDQLKRCKPIFEELPGWTEDVTNVRTLEELPEN
                     ARKYLERISELCNVQISIFSVGPDREQTNLLKELW"
BASE COUNT         8737 a         3803 c         4795 g         7691 t
ORIGIN      
        1 gacttcggga tgtgttgagt ggttaatgtt ttccttcccc taccctatcc ttacttaatc
       61 tttttattaa aaactttggc aattttaagt acgtgctcaa gactattctg tatttgtaaa
      121 gtcgtcatat ctttagctgg ctgtcttgct attacaataa tatctttggc caatatatgc
      181 gacttatgta ctttgaaatt ttcacgtatt gctcttttaa tcttgtttct taacactgca
      241 ttacctagtt ttttagaaac actaatacct aagcgaaaat ggtctatttc tttattatta
      301 caagtgtata caacaaattg tctgttggct acagaatgac cttttttata tattctctga
      361 aaatctgcat tctttttaat tcggtaagct ttttccaata acatcactcg cttatttatc
      421 gtttttattt gaagctatat ttaaacttct attgagctta taacataaat ttctatttat
      481 tcttaattta aacgaaaaaa aaagatcact gatgagtcag tgatcttatg cagataaaac
      541 tttacggcct ttacgacgac ggcgcgctaa aactttacgg ccattttttg tgctcatgcg
      601 ttttctgaaa ccatgaactt tactatgttt acgtttattt ggttgataag tacgttttac
      661 catgcaaaac acctccatct ttatacagtt atctaatata ccagattatg cagactctga
      721 tcactttaat agataagatt ttgcgtttct tagattaaaa ttctcgtatt agctcattga
      781 ttatctagtc ataattcaag caactactac aatataacaa aatcctattt ataacgcaag
      841 ttcattttat actactgctc aattttttta cttttatcga ttaaagatag aaatacacga
      901 tgcgagcaat caaatttcat aacatcacca tgagtttggt ccgaagcatg agtgtttaca
      961 atgttcgaac accttataca gttcttatac atactttata aattatttcc caaactgttt
     1021 tgatacactc actaacagat actctataga aggaaaagtt atccacttat gcacatttat
     1081 agttttcaga attgtggata attagaaatt acacacaaag ttatactatt tttagcaaca
     1141 tattcacagg tatttgacat atagaggact gaaaaagtat aattgtgtgg ataagtcgtc
     1201 caactcatga ttttataagg atttatttat tgatttttac ataaaaatac tgtgcataac
     1261 taataagcaa gataaagtta tccaccgatt gttattaact tgtggataat tattaacatg
     1321 gtgtgtttag aagttatcca cggctgttat ttttgtgtat aacttaaaaa tttaagaaag
     1381 atggagtaaa tttatgtcgg aaaaagaaat ttgggaaaaa gtgcttgaaa ttgctcaaga
     1441 aaaattatca gctgtaagtt actcaacttt cctaaaagat actgagcttt acacgattaa
     1501 agatggtgaa gctatcgtat tatcgagtat tccttttaat gcaaattggt taaatcaaca
     1561 atatgctgaa attatccaag caatcttatt tgatgttgta ggctatgaag ttaaacctca
     1621 ctttattact actgaagaat tagcaaatta tagtaataat gaaactgcta ctccaaaaga
     1681 aacaacaaaa ccttctactg aaacaactga ggataatcat gtgcttggta gagagcaatt
     1741 caatgcccat aacacatttg acacttttgt aatcggaccc ggtaaccgct ttccacatgc
     1801 agcgagttta gctgtggccg aagcaccagc caaagcgtac aatccattat ttatctatgg
     1861 aggtgttggt ttaggaaaaa cccatttaat gcatgccatt ggtcatcatg ttttagataa
     1921 taatccagat gccaaagtga tttacacatc aagtgaaaaa ttcacaaatg aatttattaa
     1981 atcaattcgt gataacgaag gtgaagcttt cagagaaaga tatcgtaata tcgacgtctt
     2041 attaatcgat gatattcagt tcatacaaaa caaggtacaa acacaagaag aatttttcta
     2101 tacttttaat gaattgcatc agaataacaa gcaaatagtt atttcgagtg atcgaccacc
     2161 aaaggaaatt gcacaattag aagaccgatt acgttcacgc tttgaatggg ggctaattgt
     2221 tgatattacg ccaccagatt atgaaactcg aatggcaatt ttgcagaaga aaattgaaga
     2281 agaaaaatta gatattccac cagaagcttt aaattatata gcaaatcaaa ttcaatctaa
     2341 tattcgtgaa ttagaaggtg cattaacacg tttacttgca tattcacaat tattaggaaa
     2401 accaattaca actgaattaa ctgctgaagc tttaaaagat atcattcaag caccaaaatc
     2461 taaaaagatt accatccaag atattcaaaa aattgtaggc cagtactata atgttagaat
     2521 tgaagatttc agtgcaaaaa aacgtacaaa gtcaattgca tatccgcgtc aaatagctat
     2581 gtacttgtct agagagctta cagatttctc attacctaaa attggtgaag aatttggtgg
     2641 gcgtgatcat acgaccgtca ttcatgctca tgaaaaaata tctaaagatt taaaagaaga
     2701 tcctattttt aaacaagaag tagagaatct tgaaaaagaa ataagaaatg tataagtagg
     2761 aaactttggg aaatgtaatc tgttatataa cagcactaat gataacaatc attttttaca
     2821 tttctatatg ctaatgtggc aagatgagca aaactcattt tgtggataat gtttaaaagt
     2881 catacacacc atacacaagt tatcaacatg tgtataactt cgccaaatct atgtttttaa
     2941 gacttatcca ccaatccaca gcacctacta ctattactaa gaacttaaaa cctatataat
     3001 tatatataaa cgactggaag gagttttaat taatgatgga attcactatt aaaagagatt
     3061 attttattac acaattaaat gacacattaa aagctatttc accaagaaca acattaccta
     3121 tattaactgg tatcaaaatc gatgcgaaag aacatgaagt tatattaact ggttcagact
     3181 ctgaaatttc aatagaaatc actattccta aaactgtaga tggcgaagat attgtcaata
     3241 tttcagaaac aggctcagta gtacttcctg gacgattctt tgttgatatt ataaaaaaat
     3301 tacctggtaa agatgttaaa ttatctacaa atgaacaatt ccagacatta attacatcag
     3361 gtcattctga atttaattta agtggcttag atccagatca atatccttta ttacctcaag
     3421 tttctagaga tgacgcaatt caattgtcgg taaaagtgct taaaaacgtg attgcacaaa
     3481 caaattttgc agtgtccacc tcagaaacac gcccagtact aactggtgtg aactggctta
     3541 tacaagaaaa tgaattaata tgcacagcga ctgactcaca ccgcttggct gtaagaaagt
     3601 tgcagttaga agatgtttct gaaaacaaaa atgtcatcat tccaggtaag gctttagctg
     3661 aattaaataa aattatgtct gacaatgaag aagacattga tatcttcttt gcttcaaacc
     3721 aagttttatt taaagttgga aatgtgaact ttatttctcg attattagaa ggacattatc
     3781 ctgatacaac acgtttattc cctgaaaact atgaaattaa attaagtata gacaatgggg
     3841 agttttatca tgcgattgat cgtgcctctt tattagcgcg tgaaggtggt aataacgtta
     3901 ttaaattaag tacaggtgat gacgttgttg aattgtcttc tacatcacca gaaattggta
     3961 ctgtaaaaga agaagttgat gcaaacgatg ttgaaggtgg tagcctgaaa atttcattca
     4021 actctaaata tatgatggat gctttaaaag caatcgataa tgatgaggtt gaagttgaat
     4081 tcttcggtac aatgaaacca tttattctaa aaccaaaagg tgacgactcg gtaacgcaat
     4141 taattttacc aatcagaact tactaaaaat aaatataaat aaaggatgac gtgattaatt
     4201 aaaacgtcat cctttatttt ttggcaaaaa taattctagg tgcgtatgta aaataaattt
     4261 ggcagcattt taaacagcaa ataaaagacg ccaattaaat ttatgacaaa tgtatccaaa
     4321 atttaataag tgtgcttata tgccctttaa atttaaaatt ttaatagtca ataacaagtt
     4381 gaatataaaa gttaaacgcc gttaaatagc gttaaaaaat tgaaaatgac agtattgcca
     4441 aaaaataaga attaattatt tatatgtaaa cggtttctac ctctatttta aatgaaattt
     4501 gtgacaaaaa aaggtataat atattaatga catacaaaga aatggagtga ttattttggt
     4561 tcaagaagtt gtagtagaag gagacattaa tttaggtcaa tttctaaaaa cagaagggat
     4621 tattgaatct ggtggtcaag caaaatggtt cttgcaagac gttgaagtat taattaatgg
     4681 agtgcgtgaa acacgtcgcg gtaaaaagtt agaacatcaa gatcgtatag atatcccaga
     4741 attacctgaa gatgctggtt ctttcttaat cattcatcaa ggtgaacaat gaagttaaat
     4801 acactccaat tagaaaatta tcgtaactat gatgaggtta cgttgaaatg tcatcctgac
     4861 gtgaatatcc tcattggaga aaatgcacaa ggaaagacaa atttacttga atcaatttat
     4921 accttagctt tagcaaaaag tcatagaacg agtaatgata aggaactcat acgttttaat
     4981 gctgattatg ctaaaataga aggtgagctt agttatagac acggcacgat gccattaaca
     5041 atgtttataa ctaaaaaagg taaacaagtc aaagtgaatc acttagagca aagtcgtcta
     5101 actcaatata ttggacacct caatgtggtt ctatttgcgc cagaagattt gaatattgta
     5161 aaaggctctc ctcaaataag acgacgcttt atagatatgg agttgggcca aatttctgct
     5221 gtttacttaa atgatttagc tcaataccaa cgtattttaa agcaaaagaa taattactta
     5281 aagcagttac aattaggcca aaaaaaggac ttaacaatgt tggaagtatt aaatcagcag
     5341 tttgctgaat atgcaatgaa agtaactgat aaacgtgcac attttattca agagctagag
     5401 tcgttagcta aaccgattca tgctggtatc acaaatgata aagaagcgtt gtcgctgaat
     5461 tatttaccta gtcttaaatt tgattatgct caaaatgaag cggcacgact tgaagaaatt
     5521 atgtctattc ttagcgataa tatgcaaaga gaaaaagaac gaggcattag cttattcgga
     5581 ccacatcgag atgatataag ttttgatgtg aatggcatgg atgctcaaac atatggttct
     5641 caaggacagc aacgtacaac ggctttgtcc attaaattag ctgaaattga gttaatgaat
     5701 atcgaagttg gggaatatcc catcttatta ttagacgatg tactcagtga attagatgat
     5761 tcgcgtcaaa cgcatttatt aagtacgatt cagcataaag tacaaacatt tgtcactacg
     5821 acatctgtag atggtattga tcatgaaatc atgaataacg ctaaattgta tcgtattaat
     5881 caaggtgaaa ttataaagta acagaaagcg atggtgactg cattgtcaga tgtaaacaac
     5941 acggataatt atggtgctgg gcaaatacaa gtattagaag gtttagaagc agtacgtaaa
     6001 agaccaggta tgtatatagg atcgacttca gagagaggtt tgcaccattt agtgtgggaa
     6061 attgtcgata atagtatcga tgaagcatta gctggttatg caaatcaaat tgaagttgtt
     6121 attgaaaaag ataactggat taaagtaacg gataacggac gtggtatccc agttgatatt
     6181 caagaaaaaa tgggacgtcc agctgtcgaa gttattttaa ctgttttaca tgctggtggt
     6241 aaatttggcg gtggcggata caaagtatct ggtggtttac atggtgttgg ttcatcagtt
     6301 gtaaacgcat tgtcacaaga cttagaagta tatgtacaca gaaatgagac tatatatcat
     6361 caagcatata aaaaaggtgt acctcaattt gacttaaaag aagttggcac aactgataag
     6421 acaggtactg tcattcgttt taaagcagat ggagaaatct tcacagagac aactgtatac
     6481 aactatgaaa cattacagca gcgtattaga gagcttgctt tcttaaacaa aggaattcaa
     6541 atcacattaa gagatgaacg tgatgaagaa aacgttagag aagactccta tcactatgag
     6601 ggcggtatta aatcgtacgt tgagttattg aacgaaaata aagaacctat tcatgatgag
     6661 ccaatttata ttcatcaatc taaagatgat attgaagtag aaattgcgat tcaatataac
     6721 tcaggatatg ccacaaatct tttaacttac gcaaataaca ttcatacgta tgaaggtggt
     6781 acgcatgaag acggattcaa acgtgcatta acgcgtgtct taaatagtta tggtttaagt
     6841 agcaagatta tgaaagaaga aaaagataga ctttctggtg aagatacacg tgaaggtatg
     6901 acagcaatta tatctatcaa acatggtgat cctcaattcg aaggtcaaac gaagacaaaa
     6961 ttaggtaatt ctgaagtgcg tcaagttgta gataaattat tctcagagca ctttgaacga
     7021 tttttatatg aaaatccaca agtcgcacgt acagtggttg aaaaaggtat tatggcggca
     7081 cgtgcacgtg ttgctgcgaa aaaagcgcgt gaagtaacac gtcgtaaatc agcgttagat
     7141 gtagcaagcc ttccaggtaa attagccgat tgctctagta aaagtcctga agaatgtgag
     7201 attttcttag tcgaagggga ctctgccggg gggtctacaa aatctggtcg tgactctaga
     7261 acgcaggcga ttttaccatt acgaggtaag atattaaatg ttgaaaaagc acgattagat
     7321 agaattttga ataacaatga aattcgtcaa atgatcacag catttggtac aggaatcggt
     7381 ggcgactttg atctagcgaa agcaagatat cacaaaatcg tcattatgac tgatgccgat
     7441 gtggatggag cgcatattag aacattgtta ttaacattct tctatcgatt tatgagaccg
     7501 ttaattgaag caggctatgt gtatattgca cagccaccgt tgtataaact gacacaaggt
     7561 aaacaaaagt attatgtata caatgatagg gaacttgata aacttaaatc tgaattgaat
     7621 ccaacaccaa aatggtctat tgcacgatac aaaggtcttg gagaaatgaa tgcagatcaa
     7681 ttatgggaaa caacaatgaa ccctgagcac cgcgctcttt tacaagtaaa acttgaagat
     7741 gcgattgaag cggaccaaac atttgaaatg ttaatgggtg acgttgtaga aaaccgtaga
     7801 caatttatag aagataatgc agtttatgca aacttagact tctaagcgct gtgaactgaa
     7861 cttttgaagg aggaactctt gatggctgaa ttacctcaat caagaataaa tgaacgaaat
     7921 attaccagtg aaatgcgtga atcattttta gattatgcga tgagtgttat cgttgctcgt
     7981 gcattgccag atgttcgtga cggtttaaaa ccagtacatc gtcgtatact atatggatta
     8041 aatgaacaag gtatgacacc ggataaatca tataaaaaat cagcacgtat cgttggtgac
     8101 gtaatgggta aatatcaccc tcatggtgac ttatctattt atgaagcaat ggtacgtatg
     8161 gctcaagatt tcagttatcg ttatccgctt gttgatggcc aaggtaactt tggttcaatg
     8221 gatggagatg gcgcagcagc aatgcgttat actgaagcgc gtatgactaa aatcacactt
     8281 gaactgttac gtgatattaa taaagataca atagatttta tcgataacta tgatggtaat
     8341 gaaagagagc cgtcagtctt acctgctcga ttccctaact tattagccaa tggtgcatca
     8401 ggtatcgcgg taggtatggc aacgaatatt ccaccacata acttaacaga attaatcaat
     8461 ggtgtactta gcttaagtaa gaaccctgat atttcaattg ctgagttaat ggaggatatt
     8521 gaaggtcctg atttcccaac tgctggactt attttaggta agagtggtat tagacgtgca
     8581 tatgaaacag gtcgtggttc aattcaaatg cgttctcgtg cagttattga agaacgtgga
     8641 ggcggacgtc aacgtattgt tgtcactgaa attcctttcc aagtgaataa ggctcgtatg
     8701 attgaaaaaa ttgcagagct cgttcgtgac aagaaaattg acggtatcac tgatttacgt
     8761 gatgaaacaa gtttacgtac tggtgtgcgt gtcgttattg atgtgcgtaa ggatgcaaat
     8821 gctagtgtca ttttaaataa cttatacaaa caaacacctc ttcaaacatc atttggtgtg
     8881 aatatgattg cacttgtaaa tggtagaccg aagcttatta atttaaaaga agcgttggta
     8941 cattatttag agcatcaaaa gacagttgtt agaagacgta cgcaatacaa cttacgtaaa
     9001 gctaaagatc gtgcccacat tttagaagga ttacgtatcg cacttgacca tatcgatgaa
     9061 attatttcaa cgattcgtga gtcagataca gataaagttg caatggaaag cttgcaacaa
     9121 cgcttcaaac tttctgaaaa acaagctcaa gctattttag acatgcgttt aagacgtcta
     9181 acaggtttag agagagacaa aattgaagct gaatataatg agttattaaa ttatattagt
     9241 gaattagaag caatcttagc tgatgaagaa gtgttattac agttagttag agatgaattg
     9301 actgaaatta gagatcgttt cggtgatgat cgtcgtacag aaattcaatt aggtggattt
     9361 gaaaacttag aggacgaaga cttaattcca gaagaacaaa tagtaattac actaagccat
     9421 aataactaca ttaaacgttt gccggtatct acatatcgtg ctcaaaaccg tggtggtcgt
     9481 ggtgttcaag gtatgaatac attggaagaa gattttgtca gtcaattggt aactttaagt
     9541 acacatgacc atgtattgtt ctttactaac aaaggtcgtg tatacaaact taaaggttac
     9601 gaagtgcctg agttatcaag acagtctaaa ggtattcctg tagtgaatgc tattgaactt
     9661 gaaaatgatg aagtcattag tacaatgatt gctgttaaag accttgaaag tgaagacaac
     9721 ttcttagtgt ttgcaactaa acgtggtgtc gttaaacgtt cagcattaag taacttctca
     9781 agaataaata gaaatggtaa gattgcgatt tcgttcagag aagatgatga gttaattgca
     9841 gttcgcttaa caagtggtca agaagatatc ttgattggta catcacatgc atcattaatt
     9901 cgattccctg aatcaacatt acgtccttta ggccgtacag caacgggtgt gaaaggtatt
     9961 acacttcgtg aaggtgacga agttgtaggg cttgatgtag ctcatgcaaa cagtgttgat
    10021 gaagtattag tagttactga aaatggttat ggtaaacgta cgccagttaa tgactatcgc
    10081 ttatcaaatc gtggtggtaa aggtattaaa acagctacga ttactgagcg taatggtaat
    10141 gttgtatgta tcactacagt aactggtgaa gaagatttaa tgattgttac taatgcaggt
    10201 gtcattattc gactagatgt tgcagatatt tctcaaaatg gtcgtgcagc acaaggtgtt
    10261 cgcttaattc gcttaggtga tgatcaattt gtttcaacgg ttgctaaagt aaaagaagat
    10321 gcagaagatg aaacgaatga agatgagcaa tctacttcaa ctgtatctga agatggtact
    10381 gaacaacaac gtgaagcggt tgtaaatgat gaaacaccag gaaatgcaat tcatactgaa
    10441 gtgattgatt cagaagaaaa tgatgaagat ggacgtattg aagtaagaca agatttcatg
    10501 gatcgtgttg aagaagatat acaacaatca tcagatgaag aataataaaa aataagactt
    10561 ccctatatgt aggggagtct tatttttatg ctagaaagta atgctttact atattcaatg
    10621 attagtaatg actaactttc taattgtttc attgcgtaag gtatttcatt gataagtctt
    10681 gatggtggca ccacatacat atcttttgca aggttttcgc caataaaact atgtgtatat
    10741 gtggcactca taaccgcttc ttttaagtta tcaaattgac cgacaaagct tgtaatcata
    10801 ccagcaagtg tatcgcccat accaccagtc gccattgctg ggctaccgat tgtcaattta
    10861 aagtcttcat ctttaaagaa aatttcagta ccatgttttt taagtacaac agttgcacct
    10921 aaacgatcaa ctgcttcacg attacgctca tatgtctgtt cctcaatagg aataccactt
    10981 aatcgttccc attctttgag gtgtggtgta aagatcacac gacatgtagg taattgcggt
    11041 ttcagtttac taaagattgt aatcgcatcg ccgtctacga ttaaattttg atgcggttgt
    11101 atattttgta gtaggaatgt aatggcatta tttcctttga aatcaacgcc aagacctgga
    11161 ccaattagta tactgtcagt catttcaatc attttcgtca acattttcgt atcattaata
    11221 tcaataacca tcgcttctgg gcaacgagaa tgtaatgctg aatgatttgt tggatgtgta
    11281 gctacagtga ttaaaccact accgctaaat acacatgcac gagccgctaa cataatggca
    11341 ccacctaagt tagcagatcc accaattaat aaaattttgc cataatcacc tttatgtgaa
    11401 tcttctttac gcttaggaat gttaatagaa tttaacgttt ccatagtgat ataacctccc
    11461 atgtaaaagc cttttccgaa tttattcaat tttaaaaata tatagtaact tttaacaaaa
    11521 tgtattataa atttctgaat tcattattat ttgtcgttaa atacaataga aaatactata
    11581 cctgtatatg caattcgtca atagataaat tattaaatat gcttacaaca atcttaatat
    11641 cctttaacgc actacaatag tgctctgata ataggttata aatgtacgta aaaccattgt
    11701 ttcaataaaa atgaaaacgt atacttcaag aaggatgggt tacttaatat aaacaagggg
    11761 gtaacatata tgactttata tttagatggt gaaacactaa caattgagga tattaaatca
    11821 tttttacaac aacaatcaaa gattgaaatt attgatgatg cgttagaacg tgtcaaaaaa
    11881 agtagagcgg tagttgaacg tattattgaa aatgaggaaa cggtttacgg tattactaca
    11941 ggttttgggt tatttagtga tgtacgtata gacccgacgc aatataatga attacaagtg
    12001 aatctgatac gctcacatgc ctgtggacta ggtgagccat tttcaaaaga agtagcatta
    12061 gtcatgatga ttttacgatt gaatacatta ttaaaaggtc attcaggtgc cactttagaa
    12121 ttagtgagac aattacaatt ttttataaat gaacgtatta taccgataat cccacagcaa
    12181 ggctctctcg gtgcatcagg agatttagcg ccattatcac atttagcatt agcattaatt
    12241 ggtgaaggga aagtattgta cagaggggaa gaaaaggata gtgacgatgt attaagagaa
    12301 ttaaatagac aacctttgaa ccttcaggct aaagaaggtt tagcattgat taatggtacg
    12361 caagctatga cagctcaagg tgtcattagt tatatagaag cagaagattt aggttaccaa
    12421 tctgaatgga ttgctgcatt aacgcatcag tctcttaatg gcattataga tgcatatcga
    12481 catgatgtgc acgcagttcg taattttcaa gaacagatta atgtggcagc gcgtatgcgt
    12541 gattggttag aaggatcaac attaacgacg cgacaatcag aaatacgtgt acaagatgca
    12601 tatacgttgc gttgtatacc acaaatccat ggcgcgagtt ttcaagtatt caattatgtt
    12661 aaacagcaat tagaatttga aatgaatgcg gctaatgata atccacttat atttgaggaa
    12721 gcaaatgaaa cgtttgttat ttcaggtggt aacttccatg gacaacctat tgcttttgca
    12781 ttagatcatc ttaaattagg tgtaagtgaa ttagcaaacg tatcggaacg tcgtctagag
    12841 cgactagtaa atcctcaatt aaatggtgat ttaccagcat ttcttagtcc agagccagga
    12901 ttgcaaagtg gcgcgatgat tatgcaatat gctgctgcaa gtctcgtttc tgaaaataaa
    12961 actttagcgc atccagcgag tgttgattct atcacttcat ctgcgaacca agaagatcac
    13021 gtatctatgg gaactacagc tgctagacat ggttatcaaa ttattgaaaa tgcaagacgt
    13081 gtgctggcaa tcgaatgtgt tattgcatta caagcagcag agttaaaagg tgttgaagga
    13141 ttatcaccaa aaacacgtcg caagtatgat gagtttcgaa gtatcgtgcc atccattaca
    13201 catgatcgtc aatttcataa agatattgaa gcggttgcac agtatttaaa gcaatcaatt
    13261 tatcaaacga ctgcatgtca ctaaatcgac atgagtgatg tttgaaatcg ttacttgaaa
    13321 aagcaaatat agtttgctat attaaaaata acttaataag acattgttcg taggacaagt
    13381 aatatatagt gttcgatatc agagagcttg tggttagtgt gaacaagaat caacatatat
    13441 atgaatctac ctacttaatt taaaagaaca atcggtgata accgttattt tagtgaagtg
    13501 caatttaggt ttagtgtatc tttataactt aaattgttaa atagggtggc aacgcgtaga
    13561 ccacgtccct tgtagggatg tggtcttttt ttattttcta aaaaataaag acgtacgtca
    13621 ttccataata aatgataatt atttgggaaa ggatgaaggt taatgttaga cattagatta
    13681 ttcagaaatg agcctgacac agttaagagc aaaattgaat tacgtggaga tgatccaaaa
    13741 gttgtagatg aaattttaga attggatgag caacgacgta aattaattag tgcaacagaa
    13801 gaaatgaaag cacgtcgtaa taaagtaagc gaagaaatcg cattaaaaaa acgtaataaa
    13861 gaaaatgctg atgatgtgat tgctgaaatg cgcacattag gtgacgatat taaagaaaaa
    13921 gatagtcaat taaatgaaat tgataataaa atgacaggta tcctttgtcg tattccaaat
    13981 ttaataagtg atgatgtacc tcaaggtgaa tctgatgaag ataacgttga agttaaaaag
    14041 tggggtacac cacgtgagtt ttcatttgaa cccaaagcac attgggatat tgtagaagaa
    14101 ttgaaaatgg ctgattttga tcgtgcagca aaagtttcag gtgcgcgttt tgtatattta
    14161 acaaatgaag gtgcgcaatt agagcgtgct ttaatgaact atatgattac aaaacataca
    14221 acacaacatg gttatacaga aatgatggta ccacagcttg tgaacgcaga tacaatgtat
    14281 ggtacaggtc aattacctaa atttgaagaa gatttattta aagtagaaaa agaaggatta
    14341 tatacaattc caactgctga agtaccatta acgaatttct accgtaatga aattattcaa
    14401 ccaggtgtac ttcctgaaaa attcactggt caatctgcat gtttccgtag tgaagcagga
    14461 tcagcaggta gagatacaag aggattaatt cgtttacatc aattcgataa agtggaaatg
    14521 gtacgttttg aacaacctga agattcatgg aatgctttag aagaaatgac aacaaacgca
    14581 gaagcaattc tagaagagtt aggtttacca taccgtcgtg ttattttatg tacaggtgat
    14641 attggattta gtgcaagcaa aacatatgat ttagaagttt ggttaccaag ctacaatgat
    14701 tataaagaaa ttagttcatg ctcaaactgt acggatttcc aagcgcgtcg tgctaacatc
    14761 cgcttcaagc gtgacaaagc agctaaacca gaattagcac atacattaaa tggtagtggt
    14821 ttagcagttg gacgtacatt tgctgctatt gttgaaaatt accaaaatga agatggaaca
    14881 gtaacaattc cagaagcatt agtaccattt atgggtggta aaacacaaat ttcaaaacca
    14941 gttaaataaa ggctttagct acaagcttta aaaagtatat atctacgtat acttaaagca
    15001 agggcaagat actttaaata atattttaaa aagtggtgac gaagctgtcg ccactttttt
    15061 tgtgctgtaa aagtataata gtgagccaat gcacataaca acaataaatt aagtttgtgg
    15121 tttaatgggg tgaacgcatt tcattatagc aacaatacgg gataattatg atgaactaaa
    15181 acaatctaaa acgtaacaag tttgagcatc accaatatag gaaaggaagc gataaaatac
    15241 tgatttcgtt gatatgtagt atgagttata tcgatggagt agggtagggg gaggggatga
    15301 ttataaggga gtggtacatg aatcaatatc ccagactcat catcagatat aaaaatttat
    15361 aaaattgata cttaaaaaca actacaaatc catagaaaat atggaggtag tcttaaataa
    15421 aaaattgaaa attctcaaaa ataaaaagtt aatatgaagc tgactaaaga ctccggtatg
    15481 tctaacctca gacaaactga tgtctaatgt tattgcttag ggtatagaac tgtattagac
    15541 taggtatatt attttttcgt aattatataa atataaagtg gcaaaggagg taattgagat
    15601 gacaacacat ttaagtttta gacaaggcgt gcaagagtgt atcccaacat tattgggtta
    15661 tgccggtgtt ggtatttcat ttggtattgt ggcttcgtct caaaacttta gtattttaga
    15721 aattgtcttg ttatgtcttg ttatatatgc cggtgctgcg caatttatta tgtgcgcgtt
    15781 gtttatagca ggtacaccta tatcagcgat tgtactaact gtatttatcg taaattcaag
    15841 aatgttcctt ttaagtatgt cgcttgcacc aaacttcaag acatatgggt tttggaaccg
    15901 tgttggatta ggttcattag taactgacga aacgtttggc gtcgccatta caccttattt
    15961 aaaaggagaa gctatcaatg atcgttggat gcatggtctt aacatcacag catatttatt
    16021 ttgggcaatt tcatgtgtag ctggggcttt atttggcgaa tatatctcaa atccgcaaac
    16081 gctagggtta gattttgcta tcacggctat gtttatcttt ttggccattg cgcaatttga
    16141 atcaattact aaatcgcgat taagaattta catagtactc attattgccg tcatagtaat
    16201 gatgttatcg ctaagtatgt ttatgccttc atatctagca atattaattg cagccacaat
    16261 ttcagcagcg ttaggagtga tgatggaaca atgataactc atatgaacat gttaatactt
    16321 attttattgt gtggtatcgt aacgctatta attcgaatta taccttttat catgatttca
    16381 aaagtgcaat tgcctgatgt cgtggttcga tggctatcat ttatcccaat cacactattt
    16441 acggcacttg tcattgacag cattattcaa cagacgcctc atggtgaggg gtatacatta
    16501 aacatccctt acattatcgc gctcattccg acggttattt tatctataat cacgcgtagt
    16561 ttaactatta caattattag tgggattgtt atcatggcag cattacgatt tttcttttaa
    16621 aaataactga aaatcattga acagatattt aaacttaggt aaactgttag taatcagaaa
    16681 attctgatat acaaatcgtc tatgttaaga ttgggaatcg tgtatacgta agcgatttgg
    16741 ctaacgcatg acaatgataa caacatttta aatgataaaa gtaattcatc actgaatctc
    16801 aactaacaca taacaatttc atatttctta ttgtgagaag ttgagggact tggccctgtg
    16861 atacttcagc aaccgacttt atagcacggt gctaaaacca acgagttact cgaatgataa
    16921 gtataaagac ttcttacttt tcaatagggt gagaagtttt tttgtttaag gaggaaagaa
    16981 caatgacaaa ttacacagta gatactttaa atttagggga atttattaca gaatctgggg
    17041 aagtcataga taacttgcgt ttgagatatg agcatgtcgg ttatcatgga caaccattag
    17101 ttgtagtttg tcatgcatta actggcaatc atttaacata tggaacagat gattatccgg
    17161 gttggtggcg agaaattatt gatgggggat atatacccat tcacgattat caatttttaa
    17221 catttgatgt tattggtagt cctttcggtt caagttcacc tttaaacgac cctcattttc
    17281 ctaaaaaatt aacattaaga gatatagtca gagcgaatga acgaggtata caagcccttg
    17341 gttatgataa gattaatatt ttaatagggg gaagtcttgg aggtatgcaa gcaatggaac
    17401 tactctacaa tcaacagttt gaagtagata aagccattat ccttgctgca acaagtcgaa
    17461 catcatctta tagtagagct ttcaatgaaa ttgcaaggca agccattcat cttggtggta
    17521 aggaaggtct aagtattgca cgccaattag gttttttgac atatcgatca tcaaaaagtt
    17581 atgatgaacg tttcacgccg gatgaagtag tcgcatacca acaacatcaa ggtaataaat
    17641 ttaaagaaca ttttgatttg aattgttatc tgacactgct agatgtattg gatagtcaca
    17701 acattgaccg aggtcgcaca gacgtaacgc atgtttttaa aaatttagaa acaaaagtgt
    17761 taacgatggg gttcatagat gatttgctat atccggatga tcaagttcgt gcattaggtg
    17821 aacgttttaa atatcatcgt catttcttcg tgcctgataa tgttgggcat gatggattcc
    17881 tactaaactt tagtacctgg gcacctaact tatatcattt cttaaattta aagcatttta
    17941 agcgtaagta ataatatata ttttcaaaga atgaagccac taaatcacag ttatcatatt
    18001 caaaaacatc tagagtacat gagagtggaa atgtattgta aatctaaatc aagcttgatt
    18061 gctatgtagt gggattaaat aatcatgttg aaaatgaaag cacacaaaag cgattcaaat
    18121 gagtatgtta attacttata aaacatgaaa gttattattc aattaaatga aatagaagca
    18181 tacaatctat aaattgttaa ttttcattaa agaggttaaa ataatagcta tagttaaaaa
    18241 tatgggagtg gcttatgtgt tttcaaaaat acaacctaaa gcaacaataa ttgcaacgat
    18301 tgcgttggta tttgtcgctt tagctttata tctagtgcct ggtttaggac taatatttgc
    18361 attatttgca accataccag gtatcgtttt atggaataaa tcaatacaat ctttcgggat
    18421 tagtgcactt attacagtaa ttataacaac tgttttaggt aatactttcg ttttaagtgc
    18481 catcatatta gtcttaattg caagtttaat tattggtcaa ttgctcaaag aaagaacgtc
    18541 taaagaaaga atattatacg taacaacagt agcgatgagc ttaatttcat taatcgcttt
    18601 tatgttacta caaacattcg gaaggattcc accatcagcg agcatagtaa aacctttcaa
    18661 gcaaacatta catgaagcga ttacgatgag cggtgccgat gcgaatatga cccaaatatt
    18721 agaagaaggg tttagacaag cgaccgttca attaccaggt ttcatcatta tcattacatt
    18781 tttaatcgtc ttaattaact taatcgttac atttccgatt ttacgaaaat ttaaaatcgc
    18841 tacacctgta tttaagccac ttttcgcgtg gcaaatgagc ggtattttat tatggatata
    18901 cattattgtt atcatatgtt tattatttac aggtcaaccg agtgtgttcc agagcattct
    18961 tttaaacttc caacttgtgt tatcattagt aatgtatatt caaggtttaa gtgttattca
    19021 tttctttggt aaagcgaaag gtttgccgaa tgcagtaacg attttactat tggttatcgg
    19081 tacaatactg acacctacga cacatattgt aggactactt ggtgttatcg atttaagttt
    19141 gaatttgaag cgaatcatga aaaataattc taaaaagtga atagaggtgg aataatgaat
    19201 cggcagtcca ctaagaaagc tttactaata ccatttgtca tcatgatcat cacagcaatt
    19261 gttttaatgg gtgtatggtt tatctttaat agtcttatag cactaattgc atctatcgtt
    19321 cttgtcgtga tgattattgt tagcatcatt ttattcagac aagctttaat gaaaatggat
    19381 agttatgtag atggtttgag tgctcaaatt tcaacaacaa ataataaagc aatcaaacat
    19441 ttaccaattg gtatcattgt tttagatgaa aatgatcaca tcgaatgggt taaccaattt
    19501 atgacagatc atatggaagc gaatgtcatt tctgaatctg taaatgaagt atttccaaac
    19561 attttaaagc aattagatag agtgaaatcc gttgaaatag aatataatca gtatcatttc
    19621 caagtacgtt attctgagaa tgatcactgc ctctatttct ttgatataac tgaacaagta
    19681 caaacaaatg aactatatga aaattctaaa ccaatcattg cgacattatt tttagataac
    19741 tacgatgaga ttacgcaaaa tatgaatgat acgcagcgtt cggaaatcaa ctcaatggta
    19801 acgcgtgtca ttagtcgatg ggcaactgag tataatatat ttttcaaaag atacagttcc
    19861 gatcaattcg tagcctattt aaatcaaaaa atattagctg acttagaaga atctaaattt
    19921 gatatcttga gtcaattacg tgaaaaaagt gttggttatc gtgcccaatt aacattaagt
    19981 atcggtgttg gtgaaggtac tgaaaattta atcgacttag gtgaattatc acaatcaggc
    20041 ctagacttag cattaggacg cggtggcgac caagttgcaa ttaaaagtat taatggtaat
    20101 gtgcgtttct atggcggtaa gactgacccg atggagaaac gtactcgtgt aagagcacgt
    20161 gtgatctcac atgcgttaaa agatatcctt gcagagggtg acaaagtcat tatcatggga
    20221 cataaacgtc ctgacttaga tgcaattggt gcagcaatcg gtgtgtctag atttgcaatg
    20281 atgaataatt tagaagcata catcgtatta aatgagactg acattgatcc aacattacga
    20341 cgcgtgatga acgaaataga taaaaagcca gagttaagag agcgatttat tacatcagat
    20401 gatgcttggg atatgatgac atctaagaca accgtagtga ttgttgatac gcataaaccg
    20461 gaactggttt tagatgaaaa tgtcttaaat aaagcaaacc gtaaagttgt tatcgatcat
    20521 catagacgtg gtgaaagctt catctctaat ccattgttga tatatatgga accatacgca
    20581 agttcgacag ctgaattggt aacagagtta ctggaatatc aaccaacaga acaacgttta
    20641 acacgtcttg aatcaacagt gatgtatgca ggtattattg tagatacaag aaactttaca
    20701 ttacgaacag gatcaagaac attcgatgca gcgagttatt tacgtgcaca tggtgcagat
    20761 acgattttaa cgcaacattt cttaaaagat gatgtggata cttacattaa tcgatctgaa
    20821 ttaattcgaa ctgtaaaagt tgaagataat ggcatagcca ttgcgcatgg ttcagacgat
    20881 aaaatttatc atccagtaac agttgcacaa gcagcagatg aactgttaag tttagaaggt
    20941 attgaagcat catatgttgt tgcgagacgt gaagataatc tgattggtat atctgcgcgt
    21001 tcactcggtt cagtaaatgt ccagttaaca atggaagcac ttggtggcgg tggacattta
    21061 accaatgcgg caacacaact taaaggtgtg acagtcgaag aggcgatagc acaattacaa
    21121 caagcaatta cagaacaatt aagtaggagt gaagatgcat gaaagtaatt tttacacaag
    21181 atgttaaagg taaaggtaaa aaaggtgaag ttaaagaagt accagtaggt tatgcaaata
    21241 acttcttatt gaaaaagaat tatgctgtag aagcaacacc aggtaacctt aaacaattag
    21301 agttacagaa aaaacgtgca aaacaagaac gccaacaaga aattgaagat gctaaagcat
    21361 taaaagaaac gttatcaaac attgaagttg aagtatcagc aaaaactggt gaaggtggta
    21421 aattgtttgg gtcagtaagt acaaaacaaa ttgccgaagc actaaaagca caacatgata
    21481 ttaaaattga taaacgtaaa atggatttac caaatggaat tcattcccta ggatatacga
    21541 atgtacctgt taaattagat aaagaagttg aaggtacaat tcgcgtacac acagttgaac
    21601 aataaagttg gattgaaata agaggtgtaa ccattcatgg atagaatgta tgagcaaaat
    21661 caaatgccgc ataacaatga agctgaacag tctgtcttag gttcaattat tatagatcca
    21721 gaattgatta atactactca ggaagttttg cttcctgagt cgttttatag gggtgcccat
    21781 caacatattt tccgtgcaat gatgcactta aatgaagata ataaagaaat tgatgttgta
    21841 acattgatgg atcaattatc gacggaaggt acgttgaatg aagcgggtgg cccgcaatat
    21901 cttgcagagt tatctacaaa tgtaccaacg acgcgaaatg ttcagtatta tactgatatc
    21961 gtttctaagc atgcattaaa acgtagattg attcaaactg cagatagtat tgccaatgat
    22021 ggatataatg atgaacttga actagatgcg attttaagtg atgcagaacg tcgaatttta
    22081 gagctatcat cttctcgtga aagcgatggc tttaaagaca ttcgagacgt cttaggacaa
    22141 gtgtatgaaa cagctgaaga gcttgatcaa aatagtggtc aaacaccagg tatacctaca
    22201 ggatatcgag atttagacca aatgacagca gggttcaacc gaaatgattt aattatcctt
    22261 gcagcgcgtc catctgtagg taagactgcg ttcgcactta atattgcaca aaaagttgca
    22321 acgcatgaag atatgtatac agttggtatt ttctcgctag agatgggtgc tgatcagtta
    22381 gccacacgta tgatttgtag ttctggaaat gttgactcaa accgcttaag aacgggtact
    22441 atgactgagg aagattggag tcgttttact atagcggtag gtaaattatc acgtacgaag
    22501 atttttattg atgatacacc gggtattcga attaatgatt tacgttctaa atgtcgtcga
    22561 ttaaagcaag aacatggctt agacatgatt gtgattgact acttacagtt gattcaaggt
    22621 agtggttcac gtgcgtccga taacagacaa caggaagttt ctgaaatctc tcgtacatta
    22681 aaagcattag cccgtgaatt agaatgtcca gttatcgcat taagtcagtt atctcgtggt
    22741 gttgaacaac gacaagataa acgtccaatg atgagtgata ttcgtgaatc tggttcgatt
    22801 gagcaagatg ccgatatcgt tgcattctta taccgtgatg attactataa ccgtggcggc
    22861 gatgaagatg atgacgatga tggtggtttc gagccacaaa cgaatgatga aaacggtgaa
    22921 attgaaatta tcattgctaa gcaacgtaac ggtccaacag gcacagttaa gttacatttt
    22981 atgaaacaat ataataaatt taccgatatc gattatgcac atgcagatat gatgtaaaaa
    23041 agtttttccg tacaataatc attaagatga taaaattgta cggtttttat tttgttctga
    23101 acgggttgat atatgttaag tttgtgtatt gaaagtgata aattagtact gtcaacgcct
    23161 ctgttaaagg gtttttagga cgttgaaaac gatttgttaa aatgattttt cttttaaaaa
    23221 ggccgaaaat caatgttcga tttttatttg cattatggtc tcgatattgg tagaatatca
    23281 aatggttaaa tgagaaaaac ttggaggtgc tcacatgtca tcaatcgtag tagttgggac
    23341 acaatgggga gacgaaggaa aaggaaaaat aacggatttc ttggcagaac agtcagatgt
    23401 tatcgcgcgt ttttcaggtg gtaataatgc aggccatacc attcaatttg gcggagaaac
    23461 atataaatta catttagtac catctggtat cttttacaaa gacaaattag cggtaatcgg
    23521 taacggagtc gttgttgatc cagttgcact attgaaagaa ttagacggat taaatgaacg
    23581 tggcattcct acaagtaatt tacgtatatc taatcgtgcg caagtgattt taccatatca
    23641 cttagcacaa gatgaatatg aagaacgttt acgtggtgac aataagattg gtacaactaa
    23701 aaaaggtatc ggtccagcat atgtagacaa agttcaacgt atcggtattc gtatggcaga
    23761 tttacttgaa aaagaaacat tcgaaagatt attaaaatca aacattgaat ataaacaagc
    23821 atatttcaaa ggtatgttta acgaaacatg tccatcattt gatgatatct ttgaagaata
    23881 ttatgcagca ggtcaacgtc taaaagaatt tgtaacagac acatcaaaaa tcttagacga
    23941 tgcatttgta gcagatgaaa aggtactttt cgaaggtgcg caaggtgtaa tgttagatat
    24001 cgaccatggt acatatccat tcgttacatc aagtaatcca attgcaggta acgttactgt
    24061 tggtacaggt gtaggtccta cattcgtttc aaaggtaatt ggtgtatgta aagcttatac
    24121 atcacgtgtt ggtgatggtc cattccctac tgaattattc gatgaagatg gacatcatat
    24181 tagagaggtt ggtcgtgaat acggtacaac aacaggacgt ccacgtcgtg taggttggtt
    24241 tgattcagtt gtattacgtc actctcgtcg tgtaagtggt attacagatt tatctattaa
    24301 ctcaatcgat gttttaacag gcctagacac agtgaaaatc tgtacagctt atgaattaga
    24361 cggtaaagaa attactgagt acccagcaaa cttagatcaa ttaaaacgtt gtaaaccaat
    24421 ctttgaagag ttaccaggtt ggacagaaga cgtaacaaat gtgcgtactt tagaagaatt
    24481 acctgaaaat gcacgtaaat atttagagcg tatttcagaa ttatgtaatg tacaaatttc
    24541 tatcttctca gttggtccag atagagaaca aacaaaccta ttaaaagaat tgtggtagaa
    24601 ctttatataa gtcatacaca atgattataa atacatgagc cttctatctt tattggtagg
    24661 aggcttttgt tatgcttgct tctgtatcga ttcgattatt tagataaaaa atactaacgt
    24721 aaaggcgata tttgctagtc ataatttaga agattagatg atatttaacg aaaattaaga
    24781 tgaaatactt gaatgtaaga agtctgatgt cgaaaatagc tattaaaata gagtagacgt
    24841 aagtgtaaat gaaagcacct aaaatagaaa aatttcaaaa atagcgtaat tattataatg
    24901 aatagactgc caataaaatg caatttttca cttataacat tcttcaaaaa ataatagcaa
    24961 aattatgtaa aaaatatctt gtcatggcaa gattggctgt gctataatct atcttgtgct
    25021 taagaa
//
LOCUS       DALAGD010000036        24848 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.036, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000036 DALAGD010000000
VERSION     DALAGD010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 24848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24848
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.036"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(<1..173)
                     /locus_tag="O2701_001183"
     CDS             complement(<1..173)
                     /locus_tag="O2701_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCZ4293086.1"
                     /translation="MTFEEKLSKIYNEIANEISSMIPVEWEKVYTMAYIDDGGGEVFF
                     NYTKPGSDDLNYYT"
     gene            complement(184..2028)
                     /locus_tag="O2701_001184"
     CDS             complement(184..2028)
                     /locus_tag="O2701_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease YeeF family protein"
                     /protein_id="HCZ4293087.1"
                     /translation="MTKDIEYLTADYDNEKSSIQSVIDAIEGQDFLDVDTTMDDAVSD
                     VSSLDEDGAISLTSSVVGPQGSKLMGYYQNELYDYASQLDSKMKEIIDTPFIEDIDKA
                     FKGITNVKLENILIKNGGGHGRDTYGASGKIAKGDAKKSDSDVYSIDEILKSDQEFVK
                     VIDQHYKEMKKEDKKLSKSDFEKMMTQGASCDYMTVAEAEELEEQKKKEEAIEIAALA
                     GMVVLSCINPVAGAVAIGAYSAYSAANAATGKNIVTGRKLSKEERIMEGLSLIPLPGM
                     GFLKGAGKSLMKLGFKGGEKFAVKTGLQKTMQQAVSRISPKMGMMKNSVLNQSRNFAQ
                     NTHVGQMLSNMRGQATHTVQQSRNWIGQQAQNVKRIVNNGLDKEIAHPFKQQLAPAGM
                     GGIKFAETTTLRNMGQNIKRAVTPQNHVTHGPKDSMVRSEGKHSISSHEMNSSKYVES
                     PNYTKVEFGEHYARLRPKKLKANIEYTTPTGHIYRTDHKGRIKEVYVDNLSLKDGDRN
                     SHAQRTVGGEDRLPDDDGGHLIARMFGGSKDIDNLVAQSKFINRPFKEKGHWYNLEKE
                     WQEFLNSGKEVKNIKMEVKYSGNSQRPTIFKVEYEINGERNIRRILNK"
     gene            complement(2038..2355)
                     /locus_tag="O2701_001185"
     CDS             complement(2038..2355)
                     /locus_tag="O2701_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293088.1"
                     /translation="MTLSGKISVKAETIAHVVKELESISQKYDEIAQNFGKIAQLNYY
                     SSEKAAHSMENGYSSAATVISGLKGPLSTLGGGVMNSAQKFFEADEHWGTEFAKLYYN
                     IEG"
     gene            complement(2355..3029)
                     /locus_tag="O2701_001186"
     CDS             complement(2355..3029)
                     /locus_tag="O2701_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5081 family protein"
                     /protein_id="HCZ4293089.1"
                     /translation="MKDVKRIDYFSYEELTILGGSKLPLVNFELFDPSNFEEAKAALI
                     EKELVTENDKLTDAGFKVATLVREYISAIVNIRINDMYFAPFSYEKDEYILLSRFKNN
                     GFQIRIINKDIAWWSIVQSYPLLMRQEKSNDWDFKQIDDETLENLNNESIDTIGRVLE
                     IEIYNHQGDPQQSLYNIYEQNDLLFIRYPLKDKVLNVHIGVINTFIRELFGFDTDENH
                     INKAEE"
     gene            complement(3029..3343)
                     /gene="esxB"
                     /locus_tag="O2701_001187"
     CDS             complement(3029..3343)
                     /gene="esxB"
                     /locus_tag="O2701_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxB"
                     /protein_id="HCZ4293090.1"
                     /translation="MGGYKGIKADGGKVDQAKQLAAKTAKDIEACQKQTQQLAEYIEG
                     SDWEGQFANKVKDVLLIMAKFQEELVQPMADHQKAIDNLSQNLAKYDTLSIKQGLDRV
                     NP"
     gene            complement(3359..3751)
                     /gene="esaC"
                     /locus_tag="O2701_001188"
     CDS             complement(3359..3751)
                     /gene="esaC"
                     /locus_tag="O2701_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion substrate EsaC"
                     /protein_id="HCZ4293091.1"
                     /translation="MNFNDIETMVKSKFKDIKKHAEEIAHEIEVRSGYLRKAEQYKRL
                     EFNLSFALDDIESTAKDVQTAKSSANKDSVTVKGKAPNTLYIEKRNLMKQKLEMLGED
                     IDKNKESLQKAKEIAGEKASEYFNKAMN"
     gene            complement(3781..8220)
                     /gene="essC"
                     /locus_tag="O2701_001189"
     CDS             complement(3781..8220)
                     /gene="essC"
                     /locus_tag="O2701_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HCZ4293092.1"
                     /translation="MHKLIIKYNKQLKMLNLRDGKTYTISEDERADITLKSLGEVIHL
                     EQNNQGTWQANHTSINKVLVRKGDLDDITLQLYTEADYASFAYPSIQDTMTIGPNAYD
                     DMVIQSLMNAIIIKDFQSIQESQYVRIVHDKNTDVYINYELQEQLTNKAYIGDHIYVE
                     GIWLEVQADGLNVLSQNTVASSLIRLTQEMPHAQADDYNTYHRSPRIIHREPTDDIKI
                     ERPPQPIQKNNTVIWRSIIPPLVMIALTVVIFLVRPIGIYILMMIGMSTVTIVFGITT
                     YFSEKKKYNKDVEKREKDYKAYLDNKSKEINKAIKAQRFSLNYHYPTVAEIKDIVETK
                     APRIYEKTSHHHDFLHYKLGIANVEKSFKLDYQEEEFNQRRDELFDDAKELYEFYTDV
                     EQAPLINDLNHGPIAYIGARHLILEELEKMLIQLSTFHSYHDLEFLFVTREDEVETLK
                     WARWLPHMTLRGQNIRGFVYNQRTRDQILTSIYSMIKERIQAVRERSRSNEQIIFTPQ
                     LVFVITDMSLIIDHVILEYVNQDLSEYGISLIFVEDVIESLPEHVDTIIDIKSRTEGE
                     LITKEKELVQLKFTPENIDNVDKEYIARRLANLIHVEHLKNAIPDSITFLEMYNVKEV
                     DQLDVVNRWRQNETYKTMAVPLGVRGKDDILSLNLHEKAHGPHGLVAGTTGSGKSEII
                     QSYILSLAINFHPHEVAFLLIDYKGGGMANLFKDLVHLVGTITNLDGDEAMRALTSIK
                     AELRKRQRLFGEHDVNHINQYHKLFKEGIATEPMPHLFIISDEFAELKSEQPDFMKEL
                     VSTARIGRSLGIHLILATQKPSGVVDDQIWSNSKFKLALKVQDRQDSNEILKTPDAAD
                     ITLPGRAYLQVGNNEIYELFQSAWSGATYDIEGDKLEVEDKTIYMINDYGQLQAINKD
                     LSGLEDEETKENQTELEAVIDHIESITTRLEIEEVKRPWLPPLPENVYQEDLVETDFR
                     KLWSDDAKEVELTLGLKDVPEEQYQGPMVLQLKKAGHIALIGSPGYGRTTFLHNIIFD
                     VARHHRPDQAHMYLFDFGTNGLMPVTDIPHVADYFTVDQEDKIAKAIRIFNDEIDRRK
                     KILSQYRVTSISEYRKLTGETIPHVFILIDNFDAVKDSPFQEVFENMMIKMTREGLAL
                     DMQVTLTASRANAMKTPMYINMKTRIAMFLYDKSEVSNVVGQQKFAVKDVVGRALLSS
                     DDNVSFHIGQPFKHDETKSYNDQINDEVSAMTEFYKGETPNDIPMMPDEIKYEDYRES
                     LNLPDIVANGALPIGLDYEGVTLQKIKLTEPAMISSENPREIAHIAEIMMKEIDILNE
                     KYAICIADSSGEFKAYRHQVANFAEEREDIKAIHQLMIEDLKQREMDGPFEKDSLYII
                     NDFKTFIDCTYIPEDDVKKLITKGPELGLNILFVGIHKELIDAYDKQIDVARKMINQF
                     SIGIRISDQQFFKFRFIQREPVIKENEAYMVANQAYQKIRWFK"
     gene            complement(8242..9576)
                     /gene="essB"
                     /locus_tag="O2701_001190"
     CDS             complement(8242..9576)
                     /gene="essB"
                     /locus_tag="O2701_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
                     /protein_id="HCZ4293093.1"
                     /translation="MVKNHNPKNEMQDMLTPLDAEEAAKTKLRLDMREIPKSSIKPEH
                     FHLMYLLEQHSPYFIDAELTELRDSFQIHYDINDNHTPFDNIKSFTKNEKLRYLLNIK
                     NLEEVNRTRYTFVLAPDELFFTRDGLPIAKTRGLQNVVDPLPVSEAEFLTRYKALVIC
                     AFNEKQSFDALVEGNLELHKGTPFETKVIEAATLDLLTAFLDEQYQKQEQDYSQNYAY
                     VRKVGHTVFKWVAIGMTTLSVLLIAFLAFLYFSVMKHNERIEKGYQAFVKDDYTQVLN
                     TYDDLDGKKLDKEALYIYAKSYIQTNKQGLEKDKKENLLNNVTPNSNKDYLLYWMELG
                     QGHLDEAINIATYLDDNDITKLALINKLNEIKNNGDLSNDKRSEETKKYNDKLQDILD
                     KEKQVKDEKAKSEEEKAKAKDEKLKQQEENEKKQKEQAQKDKEKRQEAERKK"
     gene            complement(9589..9831)
                     /gene="esaB"
                     /locus_tag="O2701_001191"
     CDS             complement(9589..9831)
                     /gene="esaB"
                     /locus_tag="O2701_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaB"
                     /protein_id="HCZ4293094.1"
                     /translation="MNQHVKVTFDFTNYNYGTYDLAVPAYLPIKNLIALVLDSLDISI
                     FDVNTQIKVMTKGQLLVENDRLIDYQIADGDILKLL"
     gene            complement(9803..10261)
                     /gene="essA"
                     /locus_tag="O2701_001192"
     CDS             complement(9803..10261)
                     /gene="essA"
                     /locus_tag="O2701_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HCZ4293095.1"
                     /translation="MLMNSVIALTFLTASSNNGGLNIDVQQEEEKRINNDLNQYDTTL
                     FNKDSKAVNDAIAKQKKERQQQIKNDMFQNQASHSTRLNETKKVLFSKSNLEKTSESD
                     KSPYIQNKQEKKIFPYILMSVGVFLTLGFVIFSIHKGRRTKNESARKSNI"
     gene            complement(10261..13290)
                     /gene="esaA"
                     /locus_tag="O2701_001193"
     CDS             complement(10261..13290)
                     /gene="esaA"
                     /locus_tag="O2701_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaA"
                     /protein_id="HCZ4293096.1"
                     /translation="MKKKNWIYALIVTLIIIIAIVSMIFFVQTKYGDQSEKGSQSVSN
                     KNNKIHIAIVNEDQPTTYNGKKVELGQAFIKRLANEKNYKFETVTRNVAESGLKNGGY
                     QVMIVIPENFSKLAMQLDAKTPSKISLQYKTAVGQKEEVAKNTEKVVSNVLNDFNKNL
                     VEIYLTSIIDNLHNAQKNVGAIMTREHGVNSKFSNYLLNPINDFPELFTDTLVNSISA
                     NKDITKWFQTYNKSLLSANSDTFRVNTDYNVSTLIEKQNSLFDEHNTAMDKMLQDYKS
                     QKDSVELDNYINALKQMDSQIDQQSSMQDTGKEEYKQTVKENLDKLREIIQSQESPFS
                     KGMIEDYRKQLTESLQDELANNKDLQDALNSIKMNNAQFAENLEKQLHDDIVKEPDTD
                     TTFIYNMSKQDFIAAGLNEDEANKYEAIVKEAKRYKNEYNLKKPLAEHINLTDYDNQV
                     AQDTSSLINDGVKVQRTETIKSNDINQLTVATDPHFNFEGDIKINGKKYDIKDQSVQL
                     DTSNKEYKVEVNGVAKLKKDAEKDFLKDKTMHLQLLFGQANRQDEPNDKKATSVVDVT
                     LNHNLDGRLSKDALSQQLSALSRFDAHYKMYTDTKGREDKPFDNKRLIDMMVDQVIND
                     MESFKDDKVAVLHQIDSMEENSDKLIDDILNNKKNTTKNKEDISKLIDQLENVKKTFA
                     EEPQEPKIDKGKNDEFNTMSSNLDKEISRISEKSTQLLSDTQESKSIADSVSGQLNQV
                     DNNVNKLHATGRALGVRANDLNRQMAKNDKDNELFAKEFKKVLQNSKDGDRQNQALKA
                     FMSNPVQKKNLENVLANNGNTDVISPTLFVLLMYLLSMITAYIFYSYERAKGQMNFIK
                     DDYSSKNHLWNNVITSGVIGTTGLVEGLIVGLIAMNKFHVLAGYRAKFILMVILTMMV
                     FVLINTYLLRQVKSIGMFLMIAALGLYFVAMNNLKAAGQGVTNKISPLSYIDNMFFNY
                     LNAEHPIGLVLVILTVLVIIGFVLNMFIKHFKKERLI"
     gene            complement(13374..13667)
                     /gene="esxA"
                     /locus_tag="O2701_001194"
     CDS             complement(13374..13667)
                     /gene="esxA"
                     /locus_tag="O2701_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxA"
                     /protein_id="HCZ4293097.1"
                     /translation="MAMIKMSPEEIRAKSQSYGQGSDQIRQILSDLTRAQGEIAANWE
                     GQAFSRFEEQFQQLSPKVEKFAQLLEEIKQQLNSTADAVQEQDQQLSNNFGLQ"
     gene            13915..14808
                     /locus_tag="O2701_001195"
     CDS             13915..14808
                     /locus_tag="O2701_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCZ4293098.1"
                     /translation="MKKTILLTMTTLTLFSMSPNSAQAYTNDSKTLEEAKKAHPNAQF
                     KVNKDTGAYTYTYDKNNTPNNNHQNQSRTNDNHQHANQRDLNNNQYHSSLSGQYTHIN
                     DAIDSHTPPQTSPSNPLTPAIPNVEDNDDELNNAFSKDNKGLITGIDLDELYDELQIA
                     EFNDKAKTADGKPLALGNGKIIDQPLITSKNNLYTAGQCTWYVFDKRAKDGHTISTFW
                     GDAKNWAGQASSNGFKVDRHPTRGSILQTVNGPFGHVAYVEKVNIDGSILISEMNWIG
                     EYIVSSRTISASEVSSYNYIH"
     gene            complement(14951..15538)
                     /locus_tag="O2701_001196"
     CDS             complement(14951..15538)
                     /locus_tag="O2701_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 family protein"
                     /protein_id="HCZ4293099.1"
                     /translation="MIIFILITIFAIYYIAMIASLFKSEGFSIIGLILDVVIMGTLIF
                     YYFIGARFVDHDLSNFLMFMDTGSYIFMYFAIKCLWVKPKVVNYLIAKELGESKEVIE
                     EQELDLQTSKIRGIYFFIISVVMLIITKLRMQPELQADAISMNPVFIFVGVIIILIWL
                     VLDIYRKKKYGIFLFKTIVPLVVTTWIIIATIILS"
     gene            complement(15572..16225)
                     /locus_tag="O2701_001197"
     CDS             complement(15572..16225)
                     /locus_tag="O2701_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HCZ4293100.1"
                     /translation="MELKVDDFVKNIKRPYLTVLGVFVVAVSLFFDAIMFFYAKLYDK
                     LPMYLLVFMAFTAVILIMMYIQEKNENYKVEKRYVVRYLTLNVIVGYTLPLLFVSIYV
                     FGVVGFGFDVFNYCLGIILMLFISWLGLFLFYKNEFDSENPNPAVNAIAIIIKLFAFG
                     GIFYISLIVPVTQQEEIFIGLSIFINIIVDALLVRSYFNYALYKSIKKDIENEGKTM"
     gene            16496..18019
                     /locus_tag="O2701_001198"
     CDS             16496..18019
                     /locus_tag="O2701_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293101.1"
                     /translation="MSKKLKIIIPIIIVLLLIGGIAWGVYAFFANTPKNTYLKSEQQT
                     AKMYKDYFNDRFENEVKFQEKMKDNSFLSSLELSADASDEIVKGLGIPKSVVNASKIK
                     MSYGHDPKKEKSMINLEPTIADSALGKFQLAADKDKHYFESPLFKGKYSVNNSDLLST
                     YSKLTGEDEETAKENGITNQQLNLNTLFNNAQAQQSDYSKIAEKYSELIVDKLDDDNF
                     DKGKKEEIKVNGEKYKVRPVTLTLSRADTKKITLAVLEEAKKDKDLKKLMEEQGATKD
                     FEKDIKKAIDDVKETKKDEFAKIQSKIYTEKHTIVKREITITDKENNKTKIKGTNTLE
                     DDKLKLDYALDFDQDKYTYAEAKYTIKGVSSKEKDNKYNDKYEFGKKTEYDESKIKLD
                     NQEKVDGTKRQDKGKITVALDKYSDENEFTFENNIDSDVKNNTQKSTLNIGIKYAEEP
                     INFILKSSTKLKADIDFDDSGAKDFNSLSSKDREKLEKEIEKNGGKMFESILKKASK"
     gene            18087..19253
                     /locus_tag="O2701_001199"
     CDS             18087..19253
                     /locus_tag="O2701_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4293102.1"
                     /translation="MKTIHLFRIYHSFLLKKWYLIIYLLFILAALLITLTTIQHVTED
                     DNHFNIGVVDKDQSSETKLILNSIGKGSNLGKNVSIKAYDDKQAHTLLKKHKLQGYFV
                     FDKGMTKAFYKQGELPISVYTYDQQSMKSVVLSQLTDSVYQRLMRSMGGILAFQDLAP
                     KASHSDSINVMTDLLITGLNRSGAFNLEPIHLYDTGSYYAITGFLTTVFIFALSLFTV
                     LKMNQDTVLKARLKMFHFSKERLLIIRTLITWFYTMLWSIVGVVWIVFSIPNIFELYN
                     WPTLAIHLSYYVTFLILWLLLIELLTTGLLNSISKVILAIVILVLSGLTIPTIFLQHI
                     ANGVFNIQPFAVVTNQLLEIILNNYILELHPSFYLSFIALLIINLAVLVWRYRQ"
     gene            19250..20170
                     /locus_tag="O2701_001200"
     CDS             19250..20170
                     /locus_tag="O2701_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4293103.1"
                     /translation="MKPYIQLVVFKQWLQYILLVTTIVIALVLIGIGYRVAHDNFKIP
                     ITIQDLDQTTASKSFVNKIKQSDYVTIKKVDEDESYIEDDVTKKEAILSMQIPKGFSQ
                     KLKENRLKETIQLYGRDDFIGGIAVEIVSSSLYEQQIPNIIYEHLEDMKQHQSIDAIN
                     KSYHKHTPESKIKFVSLTKQAQHSISISLIFAVILFVSAVQVVLHYRLNQQAALQRLS
                     QYHLSRFKLYSTYVMTHTILLLLVLLAVSLYLSQPLSLIFYLKSLLLILIYEIGIVFI
                     LFHIQTISHRLFMTFIYALAMGIVYLIIFM"
     gene            20184..20843
                     /locus_tag="O2701_001201"
     CDS             20184..20843
                     /locus_tag="O2701_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4293104.1"
                     /translation="MIEINNLSKRYRNKQIFNHLTMSFDSNRLTVLLGDNGAGKSTLL
                     RMIAGIEKANDGTINYFGEKWNQRQIQNHIGYVPQDIALFEHMTVAENIKFFKSLCKN
                     PINDTTINEYLQQLNFDDTSAKVSTLSGGNKRKINILVGLLGQPRILILDEPTVGIDL
                     KSRHDIHQLLNIMKSKCLIILTTHHLDEVEALADDIKLIGQDPFYQHVLEDKQWTYTY
                     Y"
     gene            complement(20895..21845)
                     /gene="lytM"
                     /locus_tag="O2701_001202"
     CDS             complement(20895..21845)
                     /gene="lytM"
                     /locus_tag="O2701_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-glycine endopeptidase LytM"
                     /protein_id="HCZ4293105.1"
                     /translation="MKKLTAAAIATMGFATFTMAHQADAAETTNTQQAHTQMSTQSQD
                     VSYGTYYTIDSNGDYHHTPDGNWNQAMFDNKEYSYTFVDAQGHTHYFYNCYPKNANAN
                     GSGQTYVNPATAGDNNDYTASQSQQHINQYGYQSNVGPDASYYSHSNNNQAYNSHDGN
                     GKVNYPNGTSNQNGGSASKATASGHAKDASWLTSRKQLQPYGQYHGGGAHYGVDYAMP
                     ENSPVYSLTDGTVVQAGWSNYGGGNQVTIKEANSNNYQWYMHNNRLTVSAGDKVKAGD
                     QIAYSGSTGNSTAPHVHFQRMSGGIGNQYAVDPTSYLQSR"
     gene            complement(22171..23163)
                     /locus_tag="O2701_001203"
     CDS             complement(22171..23163)
                     /locus_tag="O2701_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HCZ4293106.1"
                     /translation="MCTGFTIQTLNNQVLLGRTMDYDYPLDGSPAVTPRNYRWKSRTG
                     TTGQTQYGFIGTGTDMEGFIYGDGVNEHGVAISTQYFRGYSSYGSTHKADAMNITQNE
                     IVTWILGYTTSIEDMKQQASQIHVVAVYLNDIGEVPPLHYHVSDATGHTVEVSFKEGE
                     VVIKDNPIGVLTNHPDLNWHYSNLRQYINISPYPATANLLEGVTIEPLGNEAGTFGLP
                     GGFTSTERFVRMAFMKANIAQNNDKEMDLMNAFYLLDAVNIPIGIVRPHDADNHYTMY
                     QTVINLTTRTLYIKYYGSNELVALKLTDDLINRKDMTIFKPEKHITIRKLNDNQ"
     gene            23397..24773
                     /locus_tag="O2701_001204"
     CDS             23397..24773
                     /locus_tag="O2701_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ4293107.1"
                     /translation="MNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTS
                     PAVLNLSISLTSFATGIFMVAAGDIADKIGQLRMTYMGLIISMFASLLLIISDITALL
                     IIGRILQGLSAAILLPSTVGVLNNQFKGEHLRRAISYLMISTVGGIGLAGVIGGLIAS
                     NFGWQTNFIISIVIAFIAILLLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLT
                     QGFEQGWFSTFSIICLSIFIITTLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVL
                     NSGLGVTVVFFIYAQTHLGLSAAQSGLVTLPYAIVAVAMIRLGEKATLRFGGKLMLII
                     GPLFPVIGITIISMTQLLASQYVIAVIIGFVICAIGNGLVATPGLTIAIFSMPNEKVG
                     LATGLYKMSGTLGGAFGIALSTTVFSMLQLNYAPSVAATVTFIVSIVLMILGSLSAYM
                     IIPKTVKS"
BASE COUNT         7733 a         4528 c         3592 g         8995 t
ORIGIN      
        1 gtgtaataat tcaagtcatc actacctggt ttagtataat taaagaatac ttcacctcct
       61 ccatcatcta tataagccat tgtatatact ttttcccact ctaccggtat catactgcta
      121 atctcattcg caatttcatt gtatattttg ctaagcttct cttcaaatgt catgttggca
      181 cctctactta tttaatattc ttctaatatt tctttcacca ttaatttcat attcaacttt
      241 aaatatagtc ggtctttgac tattaccgct atattttact tccattttaa tatttttcac
      301 ctctttccca gagtttaaga actcttgcca ctctttctca agattatacc aatgaccttt
      361 ctccttaaat ggacggttga taaatttact ttgtgccaca aggttatcaa tgtcttttga
      421 accaccaaac attctagcga ttaagtgacc tccatcatcg tctggtaatc tatcctctcc
      481 ccccacagtt ctttgtgcat ggctattacg atccccatct tttagagaga gattgtctac
      541 ataaacttct tttatgcgac ctttatgatc ggttcgatat atgtgaccag taggtgttgt
      601 gtattcaata ttcgccttta gtttcttagg tctaagtctt gcatagtgtt ctccgaattc
      661 aaccttggtg tagtttggtg attcaacata ctttgatgaa ttcatttcat ggctacttat
      721 actatgtttg ccttcacttc tcaccatact atcttttgga ccatgtgtca cgtgattttg
      781 tggtgtaaca gcacgcttta tgttttgacc catgtttctc aaagtagttg tttcagcaaa
      841 ttttatacca cccattcccg ctggtgcaag ttgttgttta aatggatgtg ctatttcttt
      901 atcaagtcca ttattcacta ttcgtttgac attttgtgct tgttgtccaa tccaatttct
      961 actttgttga acagtatgag ttgcttgacc acgcatgtta ctcagcattt gtccaacatg
     1021 agtattttga gcaaagttac gagattgatt caacacactg tttttcatca ttcccatttt
     1081 aggtgaaata cgactaactg cttgttgcat tgtcttttgc aatcctgttt taactgcaaa
     1141 tttttctccg cctttgaagc ctaatttcat taaacttttc ccagcacctt tgaggaagcc
     1201 catacctggc aatggaataa gcgaaagtcc ttccatgatt cgttcttctt tagatagctt
     1261 tcttccagtt acaatatttt ttcctgtggc tgcatttgct gctgaataag cggaataagc
     1321 accaatagct actgctccag caacaggatt aatacaagat aaaactacca taccagctag
     1381 tgctgcaatc tctatagctt cttctttttt cttttgctcc tctagctctt ccgcttcagc
     1441 tactgtcatg taatcacaag aagcgccctg agtcatcatt ttttcaaaat cacttttaga
     1501 taatttctta tcttcttttt tcatttcttt gtaatgctga tcaattactt ttacaaattc
     1561 ttgatccgat tttaatattt catcgatgct ataaacatcg ctgtcacttt tcttggcatc
     1621 tccctttgca attttcccag aagccccata ggtatctcta ccatgtccac caccattttt
     1681 aattagtata ttttccaatt taacattcgt tatacctttg aatgctttat ctatatcttc
     1741 tataaatggc gtgtcaataa tttctttcat tttcgaatct aattgagatg cataatcata
     1801 taactcattt tgataatacc ccattaattt agatccttgt ggacctacta cactacttgt
     1861 taatgatatt gcgccatctt cgtctaaaga actgacatcg cttaccgcat catccattgt
     1921 tgtatctaca tctaagaagt cttgaccctc tattgcatct attacacttt ggatagatga
     1981 cttttcattg tcataatcag ctgttagata ttcaatatct tttgtcatgt catgcaccta
     2041 tccctcaata ttatagtaaa gcttggcaaa ttccgtaccc caatgttcat ctgcttcaaa
     2101 gaacttttgt gctgaattca tgacgccacc accgagtgta ctcaatggac ctttgagacc
     2161 actaatgact gttgcagcac tactatagcc attttccata gaatgtgcag ctttttcact
     2221 actgtagtaa tttaattgcg ctatttttcc aaagttttga gctatttcat catacttttg
     2281 acttatgctt tccaattctt ttacaacatg tgcaatcgtt tcagctttaa cactaatttt
     2341 tccactcaac gtcattactc ctctgcttta ttaatatgat tttcatcagt atcgaatcca
     2401 aataattctc gtataaatgt attaatgaca ccaatatgaa cattcagcac tttatctttt
     2461 aatgggtatc gaatgaataa caaatcattt tgttcataaa tgttatataa actttgttga
     2521 gggtcacctt gatgattgta tatttcaatt tctaaaacac gcccaatcgt atcgatactt
     2581 tcattattta agttctccaa tgtttcatcg tcaatttgtt taaagtccca atcattggac
     2641 ttttcttgtc tcatcaataa aggatatgat tgtacaatcg accaccatgc aatgtcttta
     2701 ttgataattc gtatttgaaa cccattattt ttaaaccggc ttaacaaaat atattcatct
     2761 ttttcatagc taaatggtgc aaaatacata tcattaattc gaatatttac aatggcgcta
     2821 atatactctc tgactaatgt agccacttta aaacctgcat cagttaactt gtcattctct
     2881 gttactaatt ccttttcaat taaagcagct ttagcttctt caaaatttga tggatcaaac
     2941 aattcaaaat ttacgagagg caatttacta ccacctaaaa ttgttaattc ttcgtaagaa
     3001 aaataatcta ttcgcttaac atctttcatc atgggttcac cctatcaagc ccttgcttaa
     3061 ttgataatgt atcgtatttc gctagatttt gacttaagtt atcaattgct ttttgatggt
     3121 cagccatcgg ttgtactaat tcttcttgaa actttgccat aatgagtaac acatctttca
     3181 ccttattggc gaactgtcct tcccaatcac taccttcgat atactcagcg agctgttgcg
     3241 tttgcttttg acatgcttca atatctttag ctgtttttgc cgctaattgt ttcgcttgat
     3301 caaccttgcc accatctgct ttaatacctt tatatccacc catatcttca cctcaatatt
     3361 aattcattgc tttattaaaa tattcacttg ccttttcgcc agcaatttcc ttagcttttt
     3421 ggagggattc tttattttta tcgatatctt cacccaacat ttcaagcttt tgtttcatca
     3481 aatttctttt ttcaatatat aacgtattgg gcgcctttcc cttaacagtt acactgtcct
     3541 tattagcact agattttgca gtttgtacgt cctttgctgt gctttcaata tcatctagtg
     3601 caaaactcaa attaaattct aatcgcttat attgttcagc ttttcttaaa tatccagaac
     3661 gaacttcaat ttcatgcgca atctcttcag catgcttttt aatatcttta aatttcgact
     3721 taaccattgt ttcaatatca ttaaaattca taacatacct ccctcctatt taattcattg
     3781 ctatttaaac catctaatct tttgataagc ttggtttgcg accatatatg cttcattttc
     3841 tttaataaca ggttctcgtt gaataaatct aaatttaaag aattgttggt ctgaaatacg
     3901 aatacctata ctaaattggt taatcatttt acgtgcaaca tcaatctgtt tatcataagc
     3961 atctattaat tctttatgaa tgccgacaaa taaaatgttc aagccaagtt ctggtccttt
     4021 tgtaataagc tttttaacat catcttccgg aatatacgtg caatcaataa atgttttaaa
     4081 atcattgata atataaagtg aatctttttc aaatgggccg tccatttctc tttgctttaa
     4141 gtcttcaatc attagttgat gaatcgcttt aatgtcttct ctttcttcgg caaagttagc
     4201 cacttgatgc ctataagctt taaactctcc acttgagtct gcgatacaaa tcgcatattt
     4261 ttcatttaat atgtcaattt ctttcatcat aatttcagca atatgcgcaa tttctctcgg
     4321 attttctgat gaaatcattg ctggttcagt taatttaatt ttttgtagtg taacaccttc
     4381 ataatctaat ccaattggta aagcaccatt tgcaactata tctggtaagt ttaatgattc
     4441 tctgtaatct tcatatttaa tttcatctgg catcatagga atatcatttg gtgtttcacc
     4501 tttataaaat tctgtcatcg ccgatacttc atcattaatt tgatcattat atgatttggt
     4561 ctcatcatgt ttaaatggtt ggccaatatg gaatgatacg ttgtcatcac tacttaacaa
     4621 tgctcgaccc acaacatctt taaccgcaaa tttttgctgt cctactacgt tcgacacctc
     4681 tgatttatca tataaaaaca ttgcgatacg cgttttcata ttaatgtaca ttggtgtttt
     4741 catagcgtta gctcttgaag cagttaaggt tacttgcatg tctaatgcta gcccttcacg
     4801 cgtcatttta atcatcatat tttcaaaaac ttcttggaaa ggtgaatctt ttactgcgtc
     4861 aaagttatca ataagaataa agacatgcgg aattgtttca ccagttaatt ttcgatattc
     4921 agaaatacta gtgacacgat actgacttaa aatcttctta cgacgatcaa tttcatcatt
     4981 aaatatacgt atcgccttag caatcttgtc ttcttgatct actgtaaagt aatcagcgac
     5041 atgtggtatg tctgtaactg gcatcaaacc attggtaccg aaatcgaaca agtacatgtg
     5101 tgcttgatca ggacgatggt gtcttgcaac atcgaaaata atgttgtgta agaacgttgt
     5161 tctaccatat cctggacttc cgattaacgc gatgtgccca gcttttttca attgcaatac
     5221 catcggtcct tgatattgtt cttctggtac gtctttaagt cctaatgtta attccacttc
     5281 ttttgcatca tctgaccata attttctgaa atctgtttct actaaatctt cttgatatac
     5341 attttctggc aatggtggta gccatggacg cttaacttct tcgatttcta atcgtgttgt
     5401 aatagattcg atatgatcta tgaccgcttc taactcagtt tgattttctt tcgtttcttc
     5461 atcttcaagt ccactcaagt ctttgttgat tgcttgaagt tgaccatagt cattaatcat
     5521 gtaaatcgtc ttatcttcaa cttctaattt atcgccttcg atgtcatatg ttgcaccact
     5581 ccatgcagat tggaataatt cataaatttc attattacca acttgtaaat acgcacgacc
     5641 tggtaatgta atgtctgctg catctggtgt ttttaaaatt tcattactgt cttgtctatc
     5701 ttgtactttt aatgccaact taaatttaga gttagaccaa atttggtcat caacaacacc
     5761 cgatggtttt tgtgtcgcaa gtattaaatg aatacctaac gaacgtccaa tacgtgccgt
     5821 tgatacaagt tctttcataa aatcaggttg ttctgatttt aattcggcaa actcatcgga
     5881 aataatgaat aaatgtggca ttggttctgt cgcaatacct tctttaaata acttatggta
     5941 ttgattaata tggttaacat catgctctcc gaataaacgt tgacgttttc tcaattcggc
     6001 tttgattgat gttaaggcac gcatcgcttc atcgccatct aagtttgtaa tcgtaccaac
     6061 taaatggact aaatctttaa ataagttcgc cataccccca cctttatagt caatcaatag
     6121 gaatgcaact tcatgagggt gaaaattaat agctaaagat aaaatgtatg attggataat
     6181 ctcagatttc cctgaaccag tggtaccagc aactaaacca tgtggcccgt gtgctttttc
     6241 atgtaagttc aatgataaaa tatcatcttt acctcttaca cctaaaggta ctgccatcgt
     6301 tttgtatgtt tcgttttgtc tccatcgatt aaccacatca agctgatcta cttctttcac
     6361 gttatacatc tctaaaaatg taatactatc aggaattgca tttttcaaat gttcgacgtg
     6421 tatcaaattc gccaaacgtc gcgcgatata ttctttatcg acgttatcaa tattttcagg
     6481 tgtaaatttc aattgaacta attctttttc tttcgtaatc agttcgcctt cagtacgaga
     6541 cttgatatca ataatggtat ctacatgctc tggcaaactt tcaatcacat cttcaacaaa
     6601 gattaatgaa ataccatatt ctgataaatc ttggtttaca tattctaaaa tgacatgatc
     6661 aataattaat gacatatctg taatgacaaa cactaattgc ggtgtgaaaa taatttgctc
     6721 attacttctg ctgcgttcac gcacagcttg gatacgttct ttaatcatgc tataaattga
     6781 cgttaaaatt tggtcacgcg ttcgttgatt gtaaacaaat cctctaatgt tttgccctct
     6841 caatgtcata tgtggcaacc aacgtgccca tttcaatgtt tcaacttcat cttcacgtgt
     6901 cacaaataga aactctaaat catgataact atggaatgtt gacaattgaa ttagcatttt
     6961 ctccaattct tctaaaatga gatgtcgtgc accaatatat gcaattggcc catgatttaa
     7021 atcattgatt aatggtgctt gttctacatc tgtgtaaaat tcatacaatt ctttagcatc
     7081 gtcgaatagt tcatcacgac gttggttaaa ttcttcttct tggtaatcta atttgaatga
     7141 cttttctaca ttcgcaatac ctaacttata atgtaagaaa tcgtgatgat gcgatgtttt
     7201 ttcatatatt cttggtgctt tcgtttcaac gatatcttta atttcagcaa ccgttggata
     7261 atggtaattc aaactaaaac gttgtgcttt aatcgctttg ttaatttctt tagatttatt
     7321 atccaaataa gctttgtaat ctttctctcg tttttcaaca tctttgttat actttttctt
     7381 ttcagagaaa tacgttgtaa taccaaatac tatcgttact gtactcatac caatcatcat
     7441 taaaatataa ataccaattg gtctcactaa aaagatgaca acagttaaag caatcattac
     7501 taatggcggt ataatggaac gccatatcac tgtattgttc ttctgtattg gctgtggcgg
     7561 tctttcaatc ttaatatcat ccgtcggttc acggtgaata atccttggcg aacgatggta
     7621 cgtattgtaa tcatctgcct gtgcatgtgg catctcttgt gttaagcgaa ttaatgacga
     7681 tgccactgta ttctgactca atacatttaa accatcagct tgtacttcga gccatatccc
     7741 ttcaacataa atatgatcac caatgtaagc tttgttcgtt agttgctctt gtagttcata
     7801 gttaatatac acatctgtat ttttatcgtg cacaatgcgt acgtattgtg attcttgtat
     7861 tgattgaaaa tctttaataa tgatggcatt catcaagctt tgaataacca tatcatcata
     7921 cgcatttggt ccaattgtca tcgtatcttg aattgaagga tacgcaaatg atgcataatc
     7981 agcttctgta taaagctgta atgtaatgtc atcaaggtca ccttttctaa caagcacctt
     8041 attaatagaa gtatgattcg cttgccaagt accttgatta ttttgttcta aatgaatgac
     8101 ttcgcctaac gatttcaacg taatatctgc acgctcgtct tcgctaatag tatatgtctt
     8161 accatctcgc aaattgagca tcttcaattg tttgttatat tttataatca atttatgcat
     8221 tgtctttgcc tcagtcctat actatttttt tctttcagct tcttggcgtt tttctttatc
     8281 tttttgtgct tgttcttttt gtttcttttc gttctcttct tgttgcttta atttctcatc
     8341 tttcgctttt gctttctctt cttcagattt cgctttttca tctttaactt gtttttcttt
     8401 gtctaaaata tcttgcaatt tatcgttata ctttttcgtt tcttcagaac gtttatcatt
     8461 cgataaatct ccgttatttt taatctcatt taatttatta atcaacgcta actttgtaat
     8521 atcgttatca tctaaataag tggcaatatt aatcgcttca tcaagatgtc cttgtcctaa
     8581 ttccatccaa tataataagt agtctttgtt tgaatttggt gtcacattat taagtaaatt
     8641 ttctttctta tctttttcta aaccttgttt atttgtttgg atataacttt tggcataaat
     8701 gtaaagtgcc tctttatcta attttttacc atctaaatca tcatacgtat ttagtacttg
     8761 cgtataatca tcctttacaa atgcttggta tcctttttca atgcgctcat tatgcttcat
     8821 tactgaaaaa tataaaaagg ctaagaatgc aattaataaa acacttaacg ttgtcatacc
     8881 gatagcaacc catttgaaaa cggtatgtcc tactttgcgt acatatgcat aattttgact
     8941 ataatcttgt tcttgtttct gatactgttc atctaaaaat gccgttagta aatctaacgt
     9001 tgccgcttca ataactttag tttcaaatgg cgttccttta tgtagttcta agtttccttc
     9061 aactaaagca tcaaatgatt gtttctcatt gaatgcacag ataaccagcg ctttatatct
     9121 tgttaaaaat tcagcttctg acacaggtaa tggatcaaca acattttgta accctcttgt
     9181 tttagcaatg ggtaatccat ctcttgtgaa aaataattca tctggtgcca acacaaatgt
     9241 gtagcgtgta cgatttactt cttctaaatt tttgatattg agtaagtaac gtaatttttc
     9301 atttttagta aatgatttaa tattatcaaa aggtgtatga ttgtcattaa tgtcataatg
     9361 tatttggaaa ctgtcacgta gttcagttaa ttcagcatct ataaaatatg gagaatgttg
     9421 ttctaataag tacattaaat gaaaatgttc tggtttaatt gaagacttag gaatctctct
     9481 catatctaag cgtaattttg ttttagctgc ttcttcagca tctaaaggcg ttaacatatc
     9541 ttgcatttca tttttagggt tatgattttt aaccatctat ttttcctcct atagtaactt
     9601 caaaatatct ccatcagcga tttgataatc aatgagtcga tcattttcaa caagtaattg
     9661 acctttcgtc atcactttaa tttgtgtatt gacatcaaat attgaaatgt ccaaactatc
     9721 caatactaaa gctattaagt tttttatcgg taaatatgct ggtactgcta agtcatatgt
     9781 gccgtaatta taattagtaa aatcaaatgt tacttttacg tgctgattca tttttcgttc
     9841 gtctcccttt atgaattgaa aaaatgacaa atcctaaagt caaaaaaacc cctacagaca
     9901 tcaaaatgta cgggaatatt tttttctcct gcttgttttg aatatagggg cttttatcac
     9961 tctccgaagt cttttctaag ttagatttgg aaaataacac ttttttagtt tcattcaagc
    10021 gagtcgagtg actcgcttga ttttgaaaca tatcattttt tatttgttgt tgtcgttctt
    10081 ttttctgctt agcaatcgca tcattaaccg ctttgctgtc tttattaaat agcgttgtat
    10141 catattgatt taaatcatta ttgattcgct tttcctcttc ttgttgcaca tcaatattaa
    10201 gtccgccatt attgctagat gctgttaaaa aagttaaagc aatcacgcta ttcatcaaca
    10261 ttagattaat ctctctttct taaagtgttt tataaacatg ttcagtacaa agccaataat
    10321 cacaagtact gttaatatta ctagcaccaa gcctatagga tgctctgcat ttaaataatt
    10381 gaagaacatg ttatcgatat aagacaatgg tgaaatttta ttagtcacac cttgtccagc
    10441 tgctttcaaa ttattcatag ctacaaagta tagacccaat gcagcaatca ttaagaacat
    10501 accgatagat tttacctgtc ttagtaaata cgtattaata agtacgaaga ccatcatagt
    10561 taaaatcacc attaagatga atttcgctct atagccagct aatacatgga acttattcat
    10621 tgcaattaaa ccgacaatta acccttctac caaaccagtt gtaccaataa cacctgacgt
    10681 aatgacatta ttccaaagat ggtttttact actataatca tctttaatga aattcatttg
    10741 tcctttggca cgttcataac tatagaaaat atatgctgta atcattgata gtaaatacat
    10801 caataatacg aataatgtcg gtgaaatcac gtctgtatta ccattattag ctaaaacatt
    10861 ttctaagttt ttcttttgaa ccggattact cataaatgct tttaatgctt ggttttgtct
    10921 gtcgccatct ttagaatttt gtaatacttt tttaaattct ttagcgaaca actcattatc
    10981 tttatcgttt ttagccattt gacgattcaa atcgttagct cttacgccta atgctcgacc
    11041 tgtcgcatgt agcttattca cattattgtc gacttgattt aattggccac taacagaatc
    11101 tgcaattgat tttgattctt gtgtatctga tagcaattgc gtactctttt cagaaattct
    11161 actaatttct ttatctaaat ttgaagacat cgtattaaat tcatcatttt tgcctttatc
    11221 aatttttggt tcttgtggct cttcagcaaa agtcttttta acgttttcta actgatcaat
    11281 cagcttggaa atatcttctt tattttttgt tgtattcttt ttgttattta aaatgtcatc
    11341 aatcagtttg tctgagtttt cttccattga atcaatttga tgtaacacag ctactttatc
    11401 gtctttgaaa ctttccatgt cattgataac ttggtcaacc atcatatcaa ttaaacgttt
    11461 gttgtcgaat ggtttatctt ctctgccttt tgtatctgtg tacattttat aatgcgcatc
    11521 aaacctagat aatgcactca attgctggct taatgcatct ttcgataagc gaccatcaag
    11581 gttatgattc aatgttacat ccacaacact cgttgctttc ttatcatttg gttcatcttg
    11641 acgatttgct tgtccaaata acaattgtaa atgcattgtt ttatctttta agaaatcttt
    11701 ctcagcatcc tttttcaatt tagcaacgcc attgacttca actttatatt ccttgttaga
    11761 tgtatcgagt tgaacacttt gatccttaat gtcatatttt ttaccattaa ttttaatgtc
    11821 gccttcaaaa ttaaaatgag gatctgttgc aacagttaat tgattaatat cattactttt
    11881 aatcgtttca gtacgttgca ctttgacacc atcattaatc aaactacttg tgtcttgcgc
    11941 aacttggtta tcgtaatctg ttaaattaat gtgttctgct aacggttttt tcaaattata
    12001 ttcattttta taacgttttg cttctttgac aattgcttcg tatttattag cttcatcctc
    12061 atttaaacct gcagctataa agtcttgttt agacatgtta tagataaatg ttgtatctgt
    12121 atcaggttct ttgacaatat catcatgaag ttgtttctct aagttttcag cgaattgagc
    12181 attgttcatt ttaatgctat ttagcgcatc ttgtaagtct ttgttatttg caagctcatc
    12241 ttgcagtgat tctgttaatt gcttacgata gtcttcaatc ataccttttg aaaatggtga
    12301 ctcttgtgat tgaatgattt ctcttaattt atctaagttt tctttaacag tttgtttata
    12361 ttcttcttta cctgtatctt gcatacttga ttgttgatca atttggctgt ccatctgttt
    12421 taatgcattg atatagttat caagttccac gctatctttt tgcgatttat aatcttgtaa
    12481 cattttatcc atcgctgtat tgtgctcgtc aaataatgaa ttttgttttt caattaaagt
    12541 cgaaacatta taatctgtgt tcactctgaa cgtatctgaa ttcgcactca ataatgattt
    12601 attgtatgtt tggaaccatt ttgtaatgtc tttgtttgca gaaattgaat ttacaagcgt
    12661 atctgtaaat aattccggga agtcgttaat tggatttaat aagtaattcg agaatttact
    12721 attcacacca tgttcacgcg tcataatagc gccaacattt ttttgtgcat tatgtaaatt
    12781 atcaatgatg cttgttaaat aaatttcgac taagtttttg ttaaagtcgt taagtacatt
    12841 acttacaact ttttctgtgt ttttagctac ttcttctttt tgtcctacag ctgttttata
    12901 ctgtagcgat attttcgatg gtgttttagc gtctaattgc attgccaatt ttgaaaagtt
    12961 ttctgggata acaatcatga cttggtatcc accatttttc aaaccagact cagcaacgtt
    13021 tcttgttact gtttcaaatt tatagttttt ctcatttgct aaccttttaa taaatgcttg
    13081 acccagctca acttttttac cgttatatgt cgttggttga tcctcgttaa caattgcgat
    13141 atgtatttta ttatttttat tacttacact ttgggatcct ttttctgatt gatctccata
    13201 ttttgtttga acaaaaaata tcatactaac tatggcaatt ataataatta aagtgacaat
    13261 taatgcataa atccaatttt tctttttcat gcttatttcc tttcagttgt tttcttaaaa
    13321 aaatgataag caaagccaca ttagaaaatg tgactttgcc aatttcagaa tgcttattgc
    13381 aaaccgaaat tattagaaag ttgttggtct tgttcttgaa cggcatcagc agtgctattc
    13441 aattgttgtt taatttcttc taataattgt gcaaattttt ctactttagg actaagttgt
    13501 tggaattgct cttcgaaacg gctgaaagct tgaccttccc agttcgctgc aatttcacct
    13561 tgtgcacgtg ttaaatcaga taaaatttga cggatttggt ctgaaccttg cccgtaagat
    13621 tgcgattttg ctctgatttc ctctggactc atcttaatca ttgccataac tagaaacctc
    13681 ctgaatattt taagtttatc aaaacttttt agggacacta ttttttgaaa aagtgctcct
    13741 tactcaaata atatataaat tattagtata tgtatatagt tttttaagta tttttagctt
    13801 tttttaaata aatatattga atatacccat atatttttaa ttaaccattc atttttgtaa
    13861 tataaatgtg tatactaaaa ttaaattaaa tacataaagg attaaatggt tattatgaag
    13921 aaaacaattt tactgacgat gacaactctt actttattta gtatgtcgcc taactcggct
    13981 caagcatata cgaatgatag caaaacatta gaagaagcaa agaaagcaca cccaaacgca
    14041 cagttcaaag tgaataaaga caccggcgcg tatacttata catatgacaa aaacaacacg
    14101 ccaaacaaca atcatcaaaa ccagtcacgt acaaacgaca atcatcaaca cgcaaatcaa
    14161 cgtgatctta acaacaatca gtaccattct tcattaagtg gtcagtatac gcacattaat
    14221 gacgcaattg attcacacac accgcctcaa acgtcaccaa gcaatccttt gacaccagca
    14281 ataccgaatg tcgaagacaa tgacgatgaa ttaaataacg ctttttcaaa agataacaaa
    14341 gggcttatta caggcatcga tttagacgaa ttgtatgacg aattacaaat cgccgaattt
    14401 aatgacaaag caaagaccgc tgacggtaaa cctttagcat taggtaacgg taaaatcatt
    14461 gatcagcctc ttatcacaag taagaacaac ttatatactg ctggacaatg tacatggtat
    14521 gtctttgata aacgtgccaa agatggacac acgattagta cattttgggg agatgctaaa
    14581 aactgggcag gccaagcttc aagcaatggc ttcaaagtag atagacaccc aacacgagga
    14641 tcaattttac aaacagtaaa tggtccattt ggtcatgtag cctacgttga aaaagttaat
    14701 attgatggaa gtattctaat ttcagaaatg aactggattg gtgaatatat cgtttcatca
    14761 agaaccatct ctgcttcaga agtttcatca tataattaca tccattaaat taatcatgac
    14821 atcaataaaa agcgaccagt tcgcagttta caattcgtaa cactgcaaaa ttggtcgctt
    14881 tattttgtat gttattcgat tataaaatta caaagaaatg ttctctacat tccccattaa
    14941 tcaaaatcgt ttacgaaagt ataattgtag ctataataat ccaagtcgta acaactagtg
    15001 gcactatcgt cttgaataag aatataccgt attttttctt gcgatatata tccagtacta
    15061 gccaaattaa aatgattata acaccaacaa aaataaatac aggattcatc gatatagcat
    15121 ctgcctgtaa ctcaggttgc attcttaatt tagtgataat taacatcact actgaaataa
    15181 tgaaaaagta gatacctctt atctttgatg tctgtaaatc taattcctgc tcttcaatga
    15241 cctctttaga ttcacccaat tcttttgcaa tcaaataatt tactacctta ggtttcaccc
    15301 ataaacactt aattgcaaag tacataaaaa tatatgatcc agtatccata aacattaaaa
    15361 agttgcttaa atcatgatca acaaagcgag cgcctataaa atagtaaaaa atcaatgtcc
    15421 ccattataac aacatctaat attaaaccta ttattgaaaa accttcactt ttaaacaaac
    15481 tagcaatcat agctatataa taaatagcaa atattgttat taaaataaaa attatcatgg
    15541 ttagactcct attatttttt atgtctttcc tttacattgt cttaccttca ttttcgatat
    15601 ctttcttaat actcttatat aacgcgtaat taaaatatga cctaacaaga agagcatcaa
    15661 caattatatt gatgaatata cttaacccta taaaaatctc ttcttgctgt gttacaggaa
    15721 ctattagact tatataaaat attcctccaa aagcaaataa ttttataata attgctatgg
    15781 cattaactgc aggattagga ttttcactat caaattcgtt tttataaaat aaaaataaac
    15841 ctaaccaaga aataaataac atcaagataa tacctagaca ataattgaaa acatcaaatc
    15901 caaaaccgac tacaccaaaa acgtaaatag atacaaaaag caatggcaaa gtatatccca
    15961 caataacgtt aagtgtgaga tatctaacca catatctttt ttcaactttg taattttcat
    16021 ttttctcttg tatgtacatc ataatcaaaa ttacagctgt aaaggccata aacaccagta
    16081 aatacatagg caatttatca tatagtttag cgtagaaaaa cataatcgcg tcaaaaaata
    16141 atgaaactgc aactacaaat actcccaata cagtcaagta tggtcttttt atattcttta
    16201 caaaatcatc gactttcaat tccataaatt cataatctcc ccaatcttac ttcaatattt
    16261 ttataagtta aataaatctt cttcgccaaa aaaatgcata ttattaagtt aaccaacatt
    16321 tttcaagaat cattatgtaa tcaaactatt ttttgtagtt tattagattt ccaataactt
    16381 attaaacatt attataacta aaattctatt aatttaaaca aaatttaaaa taaaccattt
    16441 aataaaatgt attgttctga tatgattaac tcataaataa ttgggaggaa gcaaaatgtc
    16501 taaaaagtta aaaattataa ttcctattat tattgtctta ctattaatag gtggaatcgc
    16561 atggggagtt tatgcatttt ttgctaatac accgaaaaat acatacctaa aaagtgaaca
    16621 acaaacagca aaaatgtata aagattattt taatgaccgt tttgaaaatg aagtgaagtt
    16681 ccaagaaaag atgaaagata attcgttttt atcttcatta gaattaagcg cagatgcatc
    16741 agatgaaatt gttaaagggc ttggcattcc taaatctgtt gttaatgcat cgaaaattaa
    16801 aatgtcatat ggacatgatc ctaaaaaaga gaaatcaatg attaatcttg aaccaacaat
    16861 cgcagactct gcattaggga aattccagtt agctgcagat aaagataagc attatttcga
    16921 atcaccatta tttaaaggga aatatagtgt taataattct gatttattat caacttattc
    16981 aaaacttaca ggtgaagatg aagaaacagc aaaagaaaat ggtattacaa accaacaact
    17041 aaatttaaat actcttttta ataatgctca agcacaacaa agtgactaca gcaaaattgc
    17101 cgaaaaatat tccgaactta ttgtcgacaa attagatgac gataattttg ataaaggtaa
    17161 aaaagaagaa attaaggtta atggtgaaaa gtacaaagtt agacctgtca cgttaacact
    17221 tagcagagct gacactaaaa aaattacatt agctgtatta gaagaagcta aaaaggataa
    17281 agaccttaaa aaattaatgg aagaacaagg tgctacaaaa gactttgaaa aagacatcaa
    17341 aaaagcaatt gacgatgtca aagaaactaa aaaggatgaa tttgctaaaa ttcaatctaa
    17401 aatttatacc gaaaaacata cgattgtaaa acgagaaatt actattacag acaaagaaaa
    17461 taataaaact aaaatcaaag gtactaatac tttagaagac gataagttaa aactagatta
    17521 cgcacttgat ttcgatcaag ataaatacac gtatgctgaa gcgaaatata caattaaagg
    17581 cgtatcttct aaagaaaaag ataataaata taatgataaa tacgaatttg gtaaaaagac
    17641 agaatatgat gaatcaaaaa tcaaattaga taaccaagaa aaagtagatg gcacaaaacg
    17701 tcaagataaa ggtaaaatca ctgtcgcatt agataaatat agcgacgaaa atgaattcac
    17761 ttttgaaaat aatatagatt ctgacgtaaa aaataacact cagaaatcta cgttaaatat
    17821 cggcatcaaa tatgctgaag aaccaattaa tttcatttta aaatctagca caaaattgaa
    17881 agcagatatt gattttgatg atagtggtgc aaaagatttc aatagtctat cttcaaaaga
    17941 ccgtgaaaaa cttgaaaaag aaatcgaaaa aaatggcggc aaaatgtttg aatcaatttt
    18001 aaaaaaggca tctaaataac taaaatataa aaataagatt ggaaggtcat cttgcctttc
    18061 aatcttatca ttggaggagt ggttaaatga aaacaataca tttgtttcgc atctaccact
    18121 cttttttatt gaaaaagtgg tatttgatta tctatttact atttatatta gcggcactcc
    18181 ttattacatt aacgacaatc caacatgtaa cagaagatga caatcatttt aatataggtg
    18241 tcgtagataa agatcaatca agtgaaacga aattaatctt aaactctatt ggtaaaggga
    18301 gtaacctagg aaaaaacgtg agcattaaag catatgatga taagcaagca catactttgt
    18361 taaaaaaaca taaacttcaa ggctattttg tttttgataa aggtatgacc aaggcatttt
    18421 ataaacaagg cgaactacca atttcagtat atacatatga tcaacaatcc atgaaaagtg
    18481 tcgtgctatc tcagctaaca gattctgttt accaacgtct tatgcgatca atgggtggca
    18541 tcttagcttt tcaagactta gcaccgaaag catcacattc tgacagtatc aatgttatga
    18601 ctgatttgct gattacagga ttaaaccgtt caggtgcatt taacttagaa ccgattcatt
    18661 tatacgatac gggcagttat tatgcaatta caggattttt aacaacggta ttcatctttg
    18721 cactatcttt atttacagtt ttgaaaatga atcaagatac tgtattgaaa gcgcgattga
    18781 aaatgtttca tttttctaaa gagcgtttat taatcattcg tacgttgatt acatggtttt
    18841 atactatgtt atggagtatc gttggtgtag tttggattgt gttcagtatt ccgaatatct
    18901 ttgaattata taattggcca acgttagcca ttcatttaag ctattatgtt acctttttaa
    18961 tactgtggtt attactcatc gaacttttaa caacaggctt gttgaatagt attagtaaag
    19021 tcatattagc tattgtcatc ctggtgttat ctggtttaac aatacctacg atttttttac
    19081 aacatatagc aaatggtgtt ttcaatattc aaccgttcgc agttgtaaca aatcaattgt
    19141 tagaaattat tttaaacaac tacattttag aactgcaccc tagtttctat cttagcttca
    19201 tcgcactttt gataattaat ttagctgtat tggtttggag gtatcgtcaa tgaagcctta
    19261 tatacaactt gttgtgttca agcaatggtt acaatacatc ttgctcgtaa caaccattgt
    19321 catcgcactc gtacttattg gtatcggtta ccgtgtagca catgacaact tcaaaatacc
    19381 gattaccatt caagatttag accaaaccac tgcatcaaaa tcattcgtca ataaaattaa
    19441 acaatctgac tatgtaacta ttaaaaaagt cgatgaagat gaaagctata ttgaagatga
    19501 tgttactaaa aaggaagcta ttttaagtat gcaaattcct aaaggtttct ctcaaaaatt
    19561 aaaagagaac cgtttaaaag aaacgataca gttatatggt agagatgact ttataggtgg
    19621 tattgctgta gaaattgtta gtagttcatt atatgagcag caaattccta acattattta
    19681 tgaacacctt gaggatatga aacagcatca atccatcgat gctatcaaca agtcctatca
    19741 taaacataca cctgaatcta aaatcaaatt tgtgtcgctt actaaacaag cacaacactc
    19801 tatttcaatt agcttaatct ttgcggtgat tttatttgtt agcgctgttc aagtagtact
    19861 tcattatcgt ttaaaccaac aagcagcatt gcaacgatta tcacaatatc atttaagccg
    19921 tttcaaacta tatagtactt atgtaatgac acatacgatt ttgttattgt tggtactatt
    19981 ggcagttagt ctatatttgt ctcaaccact cagcttaata ttttacttaa aatcactgtt
    20041 acttatattg atttatgaga taggtatcgt ctttatctta ttccatattc aaacaataag
    20101 tcatcgatta ttcatgacat ttatatatgc acttgctatg ggtatcgtat acttgattat
    20161 tttcatgtaa aggagcgtaa ctgatgatag aaattaataa cctttcaaag cgttaccgta
    20221 acaaacagat tttcaatcat ttaactatgt cctttgatag taatcgttta accgtattac
    20281 ttggtgataa tggtgctgga aaatcaacat tacttcgtat gattgctggt attgaaaaag
    20341 ctaatgatgg aactatcaac tatttcggcg aaaaatggaa tcaaagacaa atacaaaatc
    20401 acatcggtta tgtgccacaa gacattgcgt tatttgaaca catgacagtg gctgaaaaca
    20461 ttaaattttt taaatcactt tgtaaaaatc caattaacga tacaactatc aacgaatatt
    20521 tacagcaatt aaactttgat gatacgtctg ccaaagtatc tacattgtcc ggtgggaata
    20581 aacgtaaaat taatatatta gtaggtttac taggtcaacc tcgaattctc attttagatg
    20641 aaccgacagt tggtattgat ttaaaatcta gacatgacat ccaccaacta cttaacatca
    20701 tgaaatctaa atgtttaatt atattaacta cccatcattt agatgaagtt gaagcacttg
    20761 cagatgatat caagttaatt ggccaagatc ctttttatca acatgtttta gaggacaaac
    20821 aatggactta tacctattat taaaacgaaa aaatcccaag ctgcgtatga tatcgcaact
    20881 tgggattttc tgtattatct actttgcaag tatgacgttg ggtctactgc atattgatta
    20941 ccgatgccac cagacatacg ttggaagtgt acgtgaggcg ctgttgaatt acccgtacta
    21001 cctgaatatg caatttggtc accagctttg actttatcac cagctgaaac agttaaacga
    21061 ttattatgca tataccattg gtagttatta ctgttcgctt ctttaatcgt tacttgattg
    21121 ccgccaccat agttactcca acctgcttgt actactgtac catcagttaa tgagtaaact
    21181 ggtgaatttt caggcattgc atagtcgaca ccgtaatgcg caccaccacc gtgatattgt
    21241 ccatatggtt gtagttgttt acgacttgtt aaccagcttg cgtctttcgc atgaccacta
    21301 gctgtcgctt tacttgctga tccaccattt tgattagatg tgccattagg ataattgacc
    21361 tttccattac catcatggct gttatacgct tggttgttgt tactatgtga ataatagctc
    21421 gcgtctggac ctacatttga ttgataacca tattgattaa tatgctgttg gctttgactc
    21481 gctgtgtagt cattgttatc tcctgctgtt gctggattca catatgtttg gccgcttcca
    21541 ttggcatttg cattttttgg ataacagtta taaaaataat gcgtatgtcc ttgagcatct
    21601 acgaatgtat agctatattc tttattatca aacattgctt gattccagtt accatcaggt
    21661 gtgtgatgat aatccccatt agaatcaatt gtataataag taccataaga tacgtcttgt
    21721 gattgtgttg acatttgtgt atgtgcttgt tgggtgtttg tcgtttctgc tgcatctgct
    21781 tgatgcgcca ttgtaaatgt agcgaagccc atcgttgcaa tcgctgctgc tgttaatttt
    21841 ttcatgtata aaacatcctc cattaaagtt aaagttagtt ttcaattaaa ctgtactgca
    21901 catactaaaa gaattagaca actgagtaaa ggatttaatt ctcattttcc aactatttaa
    21961 tattcccgaa atgttttact aaactcatta cattgtcatt acaaaatagc catacattga
    22021 tattaaaatg acatctctca ctgcattcgt ttaacctttt ataaattttc aaaattaaca
    22081 actaatcgtt cgtccatgtt cgcgattcaa cgctaatgca tgatagtaat catccatcaa
    22141 atcatatcaa ccaaattcca ttatcaatcg ctattgattg tcattcaact ttctaatagt
    22201 gatatgcttc tcaggcttaa aaatcgtcat atcttttcta ttaattaaat catctgtgag
    22261 ctttaatgct actaattcat tgctgccata atacttaata tataacgttc ttgtagttaa
    22321 atttattacg gtctgataca tcgtatagtg attgtcagca tcatgcggac gtacaattcc
    22381 aatcggtata tttaccgcat ctaataaata aaatgcattc attaaatcca tttctttatc
    22441 attgttttga gcaatgtttg ctttcataaa tgccattctc acaaagcgct cagttgaagt
    22501 aaatccacct ggcaatccaa atgtacctgc ttcattgcct aaaggttcaa tcgttacacc
    22561 ttccaataaa tttgctgttg ctggataagg agaaatattg atatattgtc ttaaattact
    22621 ataatgccaa tttaagtctg gatgatttgt taagacacca ataggattat cttttataac
    22681 cacttcaccc tctttaaatg aaacttcgac tgtatgtcca gttgcatcgg aaacatgata
    22741 atgcaatggc ggaacttcac cgatgtcatt taaatataca gctacaacat gtatttggga
    22801 tgcttgttgt ttcatatctt caatgcttgt tgtatatccc aaaatccatg tcacaatttc
    22861 attttgcgta atattcatcg cgtccgcttt gtgtgttgat ccatatgaac tataacctcg
    22921 gaaatattgt gttgaaatgg caacgccatg ttcattaaca ccatcaccat aaataaaacc
    22981 ttccatatct gttcctgtgc caataaagcc atattgcgtt tggcctgtcg tgccagtgcg
    23041 agatttccaa cgataatttc taggcgtcac tgctggcgaa ccatctaatg gataatcata
    23101 atccatcgtg cgtccaagaa gtacttgatt atttaaagtt tgtattgtga atcctgtgca
    23161 cattgttctc actcctctgt accttcattt actttaatca ctttcaaata aagctgtttc
    23221 acttaaacat actataaaaa atcaattata caagcaatta attgatattc attctcaata
    23281 actgtggtat gatatgtaag gaaatcatga cttatgtgtg agtgaacgat catctataca
    23341 tccgttcact tcatctcatg actttctata tttaattttt acaaggggtg acatctgtga
    23401 ataacacaca atcttcacca cgcagtaata ttattattgc gattatgttg tctgcattaa
    23461 catattggtt gtttgcacaa tcatttatta atataggacc tctcgttggt caaacgtatc
    23521 aaacctctcc tgccgtgtta aatttatcta ttagtttaac ttccttcgcc acaggtatct
    23581 tcatggtggc tgcaggtgat attgctgata aaataggaca actgagaatg acatacatgg
    23641 gtctcataat cagtatgttt gcatctcttc tattaattat atcggacatc actgcactgc
    23701 tcatcatcgg tagaatttta caaggtctat cagcagctat cttgttacct tcaacagttg
    23761 gcgtgttaaa taatcaattt aaaggagaac atttaagacg agcgattagt tatctaatga
    23821 ttagtactgt tggtggcatc ggcctagctg gtgttatcgg cggtttaatt gcctcaaatt
    23881 tcggatggca aacgaatttc atcattagta tagtcattgc tttcattgcc atattgcttc
    23941 taaaaggcac acctgaaaaa gtaagtcaac atagccaccg tcatccattc gattacaaag
    24001 gtatgtcgat tttcgctgtt atgattggta gctttacatt attgttaaca caaggattcg
    24061 aacaaggttg gtttagtaca ttttcaatca tttgtctgag catttttatc atcactacgt
    24121 tgatattcat catcatcgaa cgtcgacatg aagtaccttt tattgatttc tcagtattac
    24181 gcaaccgtcc gttcattggt gcatttttaa ataactttgt tttaaatagc ggtctaggcg
    24241 taacagtggt ctttttcata tatgctcaaa cacaccttgg tttatcagct gcgcaatctg
    24301 gacttgttac attgccatat gccattgtgg cagttgcgat gattcgttta ggtgaaaaag
    24361 caacattacg tttcggtggc aaattgatgc tcatcattgg tccgttgttc cctgtcatcg
    24421 gcatcactat tattagcatg actcaattat tggcatcaca atatgtcatt gcagttatca
    24481 ttggtttcgt catatgtgcg ataggtaatg gtttagtcgc aacacctgga cttacgattg
    24541 caattttcag tatgcctaat gaaaaagttg gtttagctac aggattatat aaaatgagtg
    24601 gtacattagg tggcgccttt ggtatagcac taagtactac agttttcagt atgttacaac
    24661 taaactatgc accaagtgta gctgcaaccg taacatttat agtcagcatt gtattgatga
    24721 tccttggctc attgtctgca tacatgatca ttccaaaaac agttaaatct taaatataat
    24781 agaagaatta tgtttcgaaa tatctttatc actttaaaat gatatacaag aaatccaaga
    24841 aaaataag
//
LOCUS       DALAGD010000037        24222 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.037, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000037 DALAGD010000000
VERSION     DALAGD010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 24222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24222
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.037"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(187..1413)
                     /gene="ftsW"
                     /locus_tag="O2701_001205"
     CDS             complement(187..1413)
                     /gene="ftsW"
                     /locus_tag="O2701_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCZ4293108.1"
                     /translation="MKNFRSILRYIGKTSKFIDYPLLVTYIVLSLIGLVMVYSASMVP
                     ATKGTLTGGIDVPGTYFYNRQLAYVIMSFIIVFFIAFLMNVKLLSNIKVQKGMIITIV
                     SLLLLTLVIGKDINGSKSWINLGFMNLQASELLKIAIILYIPFMISKKMPRVLSKPKL
                     ILSPIVLALGCTFLVFLQKDVGQTLLILIILVAIIFYSGIGVNKVLRFGIPAVLGFLV
                     VFVIALMAGWLPSYLTARFSTLTDPFQFESGTGYHISNSLLAIGNGGVFGKGLGNSAM
                     KLGYLPEPHTDFIFAIICEELGLIGGLLVITLEFFIVYRAFQFANKTSSYFYKLVCVG
                     IATYFGSQTFVNIGGISATIPLTGVPLPFISFGGSSMISLSIAMGLLLIVGKQIKVDQ
                     QRKKQQQKVDIRRQFN"
     gene            complement(1727..2002)
                     /locus_tag="O2701_001206"
     CDS             complement(1727..2002)
                     /locus_tag="O2701_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HCZ4293109.1"
                     /translation="MAKQATMKNAALKQLTKDADEILHLIKVQLDNLTLPSCPLYEEV
                     LDTQMFGLQKEVDFAVKLGLVDREDGKQIMLRLEKELSKLHEAFTLV"
     gene            2143..2625
                     /locus_tag="O2701_001207"
     CDS             2143..2625
                     /locus_tag="O2701_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293110.1"
                     /translation="MIQIKGAVKFPITLDSTTWIFDDRKVSIEDLEKGVFDGSKPIDF
                     EDNHEWNRAILEGQTNPPTLNSEIKYKKRAMLEGSFVINMTPFFKHSEPHDSATTIRL
                     SNEHDHLDIPIDLLPYLFFQFAKDGKRLYSDNGVDSFIYNQEDGYSYRINHVTHIEVI
                     "
     gene            2627..2794
                     /locus_tag="O2701_001208"
     CDS             2627..2794
                     /locus_tag="O2701_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaI family protein"
                     /protein_id="HCZ4293111.1"
                     /translation="MRQVQCIICDAKVFIDERTTESKRLKNNPIRTFMCDDCKSRLDT
                     PKQRAQHYPLD"
     gene            complement(2934..4781)
                     /gene="typA"
                     /locus_tag="O2701_001209"
     CDS             complement(2934..4781)
                     /gene="typA"
                     /locus_tag="O2701_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HCZ4293112.1"
                     /translation="MTNKREDVRNIAIIAHVDHGKTTLVDELLKQSGIFRENEHVDER
                     AMDSNDIERERGITILAKNTAVDYKGTRINILDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLKPVVVVNKIDKPSARPEGVVDEVLDLFIELEANDEQ
                     LEFPVVYASAVNGTASLDPEKQDDNLQSLYETIIDYVPAPIDNSDEPLQFQVALLDYN
                     DYVGRIGIGRVFRGKMRVGDNVSLIKLDGTVKNFRVTKIFGYFGLKRLEIEEAQAGDL
                     IAVSGMEDINVGETVTPHDHQEALPVLRIDEPTLEMTFKVNNSPFAGREGDFVTARQI
                     QERLNQQLETDVSLKVSNTDSPDTWVVAGRGELHLSILIENMRREGYELQVSKPQVII
                     KEIDGVMCEPFERVQCEVPQENAGAVIESLGARKGEMVDMTTTDNGLTRLIFNVPARG
                     MIGYTTEFMSMTRGYGIINHTFEEFRPRIKAQIGGRRNGALISMDQGSASTYAILGLE
                     DRGVNFMEPGTEVYEGMIVGEHNRENDLTVNITKTKHQTNVRSATKDQTQTMNRPRIL
                     TLEEALQFINDDELVEVTPESIRLRKKILNKNVREKEAKRIKQMMQENE"
     gene            4883..5074
                     /locus_tag="O2701_001210"
     CDS             4883..5074
                     /locus_tag="O2701_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaF family protein"
                     /protein_id="HCZ4293113.1"
                     /translation="MKQKKSKNIFWVFSILAVVFLVLFSFAVGASNVPMMILTFILLV
                     ATFGIGFTTKKKYRENDWL"
     gene            complement(5228..6055)
                     /locus_tag="O2701_001211"
     CDS             complement(5228..6055)
                     /locus_tag="O2701_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HCZ4293114.1"
                     /translation="MALYGFAQGLIQEAGIRIKQLMEQNLTIETKSNPNDLVTNVDKA
                     TEDFIFDTILETYPNHQVLGEEGHGHDIDTSKGTVWIVDPIDGTLNFVHQQENFAISI
                     GIYIDGKPYAGFVYDVMADVLYHAKVGEGAYRGSQPLKPLNDSNLRQSIIGINPNWLT
                     KPILGEIFKEIVNDSRSARAYGSAALEIVSVATGNLEAYMTPRLQPWDFAGGLVILYE
                     VNGQASNLLGEPLTISGPNSILVGNRGLHQEISNDYLEPHHDALIQLHEQRFKRKSK"
     gene            6212..6826
                     /locus_tag="O2701_001212"
     CDS             6212..6826
                     /locus_tag="O2701_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YktB family protein"
                     /protein_id="HCZ4293115.1"
                     /translation="MTKYTFKPKDFKAFNVEGLDARMEALNEYIRPQLRELGEYFSDF
                     FTSQTGETFYPHVAKHARRSVNPPKDTWVAFATNKRGYKMLPHFQIGMFEDQLFVMFG
                     IMHEAKDKATRAKVFERKFKAIQQLPDDYRVCLDHMKPDKPFIKDLTDDDLIEAIQRA
                     INVKKGEFFIARAITPQDKRLKSDKAFIAFLEETFDQFLPFYSA"
     gene            7010..8362
                     /locus_tag="O2701_001213"
     CDS             7010..8362
                     /locus_tag="O2701_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCZ4293116.1"
                     /translation="MNNKRHSTNEQLSLDEINNTIKFDHRSSNKQKFLSFLGPGLLVA
                     VGYMDPGNWITSMQGGAQYGYTLLFVILISSLSAMLLQSMTVRLGIATGMDLAQMTRH
                     YLSRPIAIIFWIIAELAIIATDIAEVIGSAIALNLLFNIPLIVGALITVLDVFLLLFI
                     MKYGFRKIEAIVGTLIFTVLFIFIFEVYISSPQLNAVLNGFIPHSEIITNNGILYIAL
                     GIIGATIMPHNLYLHSSIVQSRTYSRHNNEEKAQAIKFATIDSNIQLSIAFVVNCLLL
                     VLGASLFFNSNADDLGGFYDLYHALKTEPVLGATMGAIMSTLFAVALLASGQNSTITG
                     TLAGQIVMEGFLRLHIPNWLRRLITRSLAVIPVIVCLIIFKGNAAKIEQLLVFSQVFL
                     SIALPFCLIPLQLATSNKDLMGPFYNKTWVNIISWTLIIILSILNVYLIVQTFQELQS
                     "
     gene            complement(8444..8863)
                     /locus_tag="O2701_001214"
     CDS             complement(8444..8863)
                     /locus_tag="O2701_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCZ4293117.1"
                     /translation="MNRKPELIMAWIANSISIIYLLVMGLSYFSLKSGNASQREELAK
                     QLSQNGGKVSLDMLQTTMGALAIILLISTLYGIFATICIKGRRKLSIILFVIAIIVSL
                     MALNLIAIVLWVIVMIMLISKKESKETTHKDDEYIYH"
     gene            complement(9146..10165)
                     /locus_tag="O2701_001215"
     CDS             complement(9146..10165)
                     /locus_tag="O2701_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCZ4293118.1"
                     /translation="MTGEQFTQIKRPVSRLTEKVLGWLCWVMLLVLTVITMFIALVSF
                     SNNTSIANLENTLNNNAFIQQLLAGNGYNTTQFVIWLQNGIWAIIVYFIVCLLISFLA
                     LISMNIRILSGFLFLISAIVTIPLVLLIVTLIIPILFFIIAMMLFIRKDKVEMVAPQY
                     YEEYNGPIYDYREPVYERPQPKDDYYDVPKYEKELDKSNTVYDQEQERDKYDQFPKRA
                     VESEYNHDERTEEEPSVLSRQAKYKQKSTEELGIEDDGYYAEPEVDPKELKAQQKREK
                     AEIKAKKKEKRKAYNQRMKERRKNQPSAVSQRRMNFEERRQIYNNDISEERNSSEVKD
                     KKEQE"
     gene            complement(10239..11312)
                     /locus_tag="O2701_001216"
     CDS             complement(10239..11312)
                     /locus_tag="O2701_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HCZ4293119.1"
                     /translation="MKRFLQLIIGALVVGMLCLTLSHWFKSKEQVHTNQKIYVYNWGE
                     YIDPELIKKFEKETGIQVVYETFDSNEAMEAKIRNGGTHYDVAFPSEYTVQKLKRDHL
                     LLPIDHNKVPNIKNLDSDYMNMSFDRGNKYSLPYFFGTVGILYNKEKYPNESFDSWKS
                     LYNPKFKNQILLVDGAREIIGMSLNKLGYNLNDRNSHHLKEAERDLTKLAPQVRGVVG
                     DEITMMLQQNEGNIAVVWSGVAAPLVQEGDKYNYVIPKEGSNLWFDNMVIPKTAQNKE
                     GAYKFMNFLLDAKNNKQNTEFVGYATPNKAARQLLPKEIKDDHRFYPTKKEQERLEVY
                     KDLGPEVLSEYNENFLNFKMSLK"
     gene            complement(11312..12121)
                     /locus_tag="O2701_001217"
     CDS             complement(11312..12121)
                     /locus_tag="O2701_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4293120.1"
                     /translation="MKWYGKLYIGILLAILYIPIFFLMFYSFNSAGNMIHFEHFTLEH
                     YQSLFQNDHLMSVIFNTIAVALLAASISTVIGTFGAIAIYYLRNKKFKVTLLTLNNVL
                     MVSSDVVIGASFLIMFTTIGHFTGLGLGFWTVLISHIAFCIPIVVIIVLPQLYEMNNN
                     MLNAARDLGATEPQLLSNIIIPNILPSIIGGFFMALTYSLDDFTVSFFVTGNGFSVLS
                     VEVYAMARKGISMEINAISTLLFAVIVLGILGYYLIQYVINKKKLIKRGVK"
     gene            complement(12127..12924)
                     /locus_tag="O2701_001218"
     CDS             complement(12127..12924)
                     /locus_tag="O2701_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4293121.1"
                     /translation="MRNTNKFLLIPYLLWMVIFIIVPVVLLIYFSFLDINGHFSFTNY
                     QQIFTTKYLKMFAYSILYAASITIITLTISYPAAYYITRSKFQNILLMIMIIPTWINL
                     LLKTYAFIGLLSHDGVINQFFHLFNLPSFNLLFTTGAFLVVASYIYIPFMILPIFNSM
                     KAIPNNLLQASSDLGASPFYTFRKVIMPLTKEGVMTGIQVTFIPSLSLFMITRLIAGN
                     KVINIGTAIEEQFLTIQNYGMGSTIAIFLIVFMAFILIITKSSNGRG"
     gene            complement(12917..14011)
                     /locus_tag="O2701_001219"
     CDS             complement(12917..14011)
                     /locus_tag="O2701_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4293122.1"
                     /translation="MEPLLSLKSVSKSYDDLNILDDIDIDIESGYFYTLLGPSGCGKT
                     TILKLIAGFEYPDSGEVIYQNKPIGNLPPNKRKVNTVFQDYALFPHLNVYDNIAFGLK
                     LKKLSKTEIDQKVTEALKLVKLSGYEKRNINEMSGGQKQRVAIARAIVNEPEILLLDE
                     SLSALDLKLRTEMQYELRELQSRLGITFIFVTHDQEEALALSDFLFVLKDGKIQQFGT
                     PTDIYDEPVNRFVADFIGESNIVEGRMVRDYVVNIYGQDFECVDMGIPENKKVEVVIR
                     PEDISLIKAEEGLFKATVDSMLFRGVHYEICCIDNKGYEWVIQTTKKAEVGSEVGLYF
                     DPEAIHIMVPGETEEEFDKRIESYEEVDNA"
     gene            complement(14024..14563)
                     /locus_tag="O2701_001220"
     CDS             complement(14024..14563)
                     /locus_tag="O2701_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ4293123.1"
                     /translation="MNIGNKIKNLRRIKNLTQEELAERTDLSKGYISQIESEHASPSM
                     ETFLNIIEVLGTTPSEFFKDSENEKVLYKKEEQVIYDEYDEGYILNWLVSKSNEYDME
                     PLILTLKPGASYKNFNPSESDTFIYCMSGQITLNLGKEIYQAQEEDVLYFKARDNHRL
                     SNESNNETRILIVATASYL"
     gene            complement(14707..14982)
                     /locus_tag="O2701_001221"
     CDS             complement(14707..14982)
                     /locus_tag="O2701_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HCZ4293124.1"
                     /translation="MEYEYPIDLDWSNEEMISVINFFNHVEKYYESGVTAGDFMGAYK
                     RFKEIVPAKAEEKQIFNTFEKSSGYNSYKAVQDVKTHSEEQRVTAKK"
     gene            complement(15150..16556)
                     /gene="lpdA"
                     /locus_tag="O2701_001222"
     CDS             complement(15150..16556)
                     /gene="lpdA"
                     /locus_tag="O2701_001222"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCZ4293125.1"
                     /translation="MVVGDFPIETDTIVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLG
                     GVCLNVGCIPSKALLHASHRFVEAQHSENLGVIAESVSLNFQKVQEFKSSVVNKLTGG
                     VEGLLKGNKVNIVKGEAYFVDNNSLRVMDEKSAQTYNFKNGIIATGSRPIEIPNFKFG
                     KRVIDSTGALNLQEVPGKLVVVGGGYIGSELGTAFANFGSEVTILEGAKDILGGFEKQ
                     MTQPVKKGMKEKGVEIVTEAMAKSAEETDNGVKVTYEAKGEEKTIEADYVLVTVGRRP
                     NTDELGLEELGVKFADRGLLEVDKQSRTSISNIYAIGDIVPGLPLAHKASYEAKVAAE
                     AIDGQAAEVDYIGMPAVCFTEPELATVGYSEAQAKEEGLAIKASKFPYAANGRALSLD
                     DTNGFVKLITLKEDDTLIGAQVVGTGASDIISELGLAIEAGMNAEDIALTIHAHPTLG
                     EMTMEAAEKAIGYPIHTM"
     gene            complement(16560..17852)
                     /locus_tag="O2701_001223"
     CDS             complement(16560..17852)
                     /locus_tag="O2701_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HCZ4293126.1"
                     /translation="MAFEFRLPDIGEGIHEGEIVKWFVKAGDTIEEDDVLAEVQNDKS
                     VVEIPSPVSGTVEEVMVEEGTVAVVGDVIVKIDAPDAEDMQFKGHDDDSSSKEEPAKE
                     EAPAEQAPVATQTEEVDENRTVKAMPSVRKYAREKGVNIKAVSGSGKNGRITKEDVDA
                     YLNGGAPTASNESAASATSEEVAETPAAPAAVTLEGDFPETTEKIPAMRRAIAKAMVN
                     SKHTAPHVTLMDEIDVQALWDHRKKFKEIAAEQGTKLTFLPYVVKALVSALKKYPALN
                     TSFNEEAGEIVHKHYWNIGIAADTDRGLLVPVVKHADRKSIFQISDEINELAVKARDG
                     KLTADEMKGATCTISNIGSAGGQWFTPVINHPEVAILGIGRIAQKPIVKDGEIVAAPV
                     LALSLSFDHRQIDGATGQNAMNHIKRLLNNPELLLMEG"
     gene            complement(17943..18920)
                     /locus_tag="O2701_001224"
     CDS             complement(17943..18920)
                     /locus_tag="O2701_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017637695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCZ4293127.1"
                     /translation="MAQMTMVQAINDALKTELKNDQDVLIFGEDVGVNGGVFRVTEGL
                     QKEFGEDRVFDTPLAESGIGGLAMGLAVEGFRPVMEVQFLGFVFEVFDAIAGQIARTR
                     FRSGGTKTAPVTIRSPFGGGVHTPELHADNLEGILAQSPGLKVVIPSGPYDAKGLLIS
                     SIRSNDPVVYLEHMKLYRSFREEVPEEEYTIDIGKANVKKEGNDISIITYGAMVQESM
                     KAAEELEKDGYSVEVIDLRTVQPIDVDTIVASVEKTGRAVVVQEAQRQAGVGAAVVAE
                     LSERAILSLEAPIGRVAAADTIYPFTQAENVWLPNKNDIIEKAKETLEF"
     gene            complement(18924..20036)
                     /gene="pdhA"
                     /locus_tag="O2701_001225"
     CDS             complement(18924..20036)
                     /gene="pdhA"
                     /locus_tag="O2701_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002467471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HCZ4293128.1"
                     /translation="MAPKLQAQFDAVKVLNDTQSKFEMVQILDENGNVVNEDLVPDLT
                     DEQLVELMERMVWTRILDQRSISLNRQGRLGFYAPTAGQEASQLASQYALEKEDYILP
                     GYRDVPQIIWHGLPLTEAFLFSRGHFKGNQFPEGVNALSPQIIIGAQYIQAAGVAFAL
                     KKRGKNAVAITYTGDGGSSQGDFYEGINFAAAYKAPAIFVIQNNNYAISTPRSKQTAA
                     ETLAQKAIAVGIPGIQVDGMDALAVYQATKEARDRAVAGEGPTLIETMTYRYGPHTMA
                     GDDPTRYRTSDEDAEWEKKDPLVRFRKFLENKGLWNEDKENEVIERAKADIKAAIKEA
                     DNTEKQTVTSLMEIMYEDMPQNLAEQYEIYKEKESK"
     gene            complement(20207..20833)
                     /locus_tag="O2701_001226"
     CDS             complement(20207..20833)
                     /locus_tag="O2701_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkyA family protein"
                     /protein_id="HCZ4293129.1"
                     /translation="MKFGKTIAVVLASSVLLAGCTTDKKEIKAYLKQVDKIKDDEEPI
                     KTVGKKIAELDEKKKKLTEDVNSKDTAVRGKAVKDLIKNADDRLKEFEKEEDAIKKSE
                     QDFKKAKSHVDNIDNDVKRKEVKQLDDVLKEKYKLHSDYAKAYKKAVNSEKTLFKYLN
                     QNDATQQGVNEKSKAIEQNYKKLKEVSDKYTKVLNKVGKEKQDVDQFK"
     gene            21198..21749
                     /locus_tag="O2701_001227"
     CDS             21198..21749
                     /locus_tag="O2701_001227"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCZ4293130.1"
                     /translation="MLTMKDIIRDGHPTLRQKAAELELPLTKEEKETLIAMREFLVNS
                     QDEEIAKRYGLRSGVGLAAPQINISKRMIAVLIPDDGSGKSYDYMLVNPKIVSHSVQE
                     AYLPTGEGCLSVDDNVAGLVHRHNRITIKAKDIEGNDIQLRLKGYPAIVFQHEIDHLN
                     GVMFYDHIDKDHPLQPHTDAVEV"
     gene            22079..22201
                     /locus_tag="O2701_001228"
     CDS             22079..22201
                     /locus_tag="O2701_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293131.1"
                     /translation="MNDMMNLVINQIVIKLHYRDDWNEFIDIYSNTILLGLTIN"
     gene            22230..22448
                     /locus_tag="O2701_001229"
     CDS             22230..22448
                     /locus_tag="O2701_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HCZ4293132.1"
                     /translation="MAVFKVFYQHNRDEVIVRENTQSLYVEAQTEEQVRRYLKDRNFN
                     IEFITKLEGAHLDYEKENSEHFNVEIAK"
     gene            22448..24145
                     /locus_tag="O2701_001230"
     CDS             22448..24145
                     /locus_tag="O2701_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCZ4293133.1"
                     /translation="MKQLHPNEVGVYALGGLGEIGKNTYAVEYKDEIVIIDAGIKFPD
                     DNLLGIDYVIPDYTYLVQNQDKIVGLFITHGHEDHIGGVPFLLKQLNIPIYGGPLALG
                     LIRNKLEEHHLLRTAKLNEINEDSVIKSKHFTISFYLTTHSIPETYGVIVDTPEGKVV
                     HTGDFKFDFTPVGKPANIAKMAQLGEEGVLCLLSDSTNSLVPDFTLSEREVGQNVDKI
                     FRNCKGRIIFATFASNIYRVQQAVEAAIKNNRKIVTFGRSMENNIKIGMELGYIKAPP
                     ETFIEPNKINTVPKHELLILCTGSQGEPMAALSRIANGTHKQIKIIPEDTVVFSSSPI
                     PGNTKSINRTINSLYKAGADVIHSKISNIHTSGHGSQGDQQLMLRLIKPKYFLPIHGE
                     YRMLKAHGETGVECGVEEDNVFIFDIGDVLALTHDSARKAGRIPSGNVLVDGSGIGDI
                     GNVVIRDRKLLSEEGLVIVVVSIDFNTNKLLSGPDIISRGFVYMRESGQLIYDAQRKI
                     KTDVISKLNQNKDIQWHQIKSSIIETLQPYLFEKTARKPMILPVIMKVNEQKESNNK"
BASE COUNT         7872 a         4339 c         3500 g         8511 t
ORIGIN      
        1 ataaaatttg ataatcatga gtaatctcgt aatatcaaaa caaaaaggaa gttaagcgtt
       61 gtttggttgc ctaacttccg ttattgaact catcagtcca atttgacaaa gagtatataa
      121 atagccaatg tttagcattg cttaatgaca gaagtcatct cgtactaaat attggctagt
      181 atttttttaa ttaaattgtc ttcttatatc aactttttgt tgttgtttct ttcgttgctg
      241 gtctactttg atttgtttac ctacaatcag aagtaaaccc atagcaatac ttaaactaat
      301 cattgatgat ccaccaaagc tgataaatgg caatggcaca ccagttaatg gaattgttgc
      361 cgaaataccg ccaatgttta caaacgtttg acttccaaag tatgtggcaa tcccaacaca
      421 cacaagttta taaaaatatg atgatgtttt atttgcaaac tggaaggcac gatatacaat
      481 aaagaactct aaagtaataa ctagcaatcc tccgattaaa cctaattctt cgcaaataat
      541 tgcaaaaata aaatctgtat gtggttctgg taaatagccc aatttcattg cactatttcc
      601 taatcctttt ccaaatacgc caccgttacc tatcgcaagc aatgaattgg aaatatggta
      661 tccagttcct gattcgaatt ggaatggatc tgttagcgta ctaaatctgg cagttaaata
      721 acttggtaac caaccagcca ttaatgcaat gacaaatact actaagaatc ctagcactgc
      781 tggtatacca aatcttagga ctttgtttac cccaattcct gaataaaaaa tgatcgcaac
      841 taaaataatt aatattagta atgtttgccc tacgtctttt tgtaggaaaa ctaaaaacgt
      901 acaacctaat gctaatacaa taggacttaa aattaatttt ggtttactta atactctagg
      961 cattttttta ctgatcataa atgggatata taatatgatt gcaattttta ataactcaga
     1021 tgcctgtaag ttcataaatc ctaagtttat ccaactttta gaaccattaa tatctttacc
     1081 tattactaac gtcagtaata atagtgagac gatagttata atcatacctt tttgcacttt
     1141 aatattactc agtaatttaa cattcattaa aaatgcaata aaaaatacaa ttataaaact
     1201 cattatgaca tatgctaatt gtcggttgta aaaatacgtt cctggaacat cgataccacc
     1261 agtcaatgtg cctttagttg ctggaaccat acttgcacta tataccatca ctaatccaat
     1321 taaactcaat acaatatatg taactaataa cggataatca ataaactttg aggttttacc
     1381 aatataccgt aaaatacttc taaaattctt catatattca gtcatccaat tctacaattt
     1441 atacttcaaa tgatttcgtt ttatacttta attttaaaaa tccatgttac ttattatata
     1501 catgacacag tcataatcac aaatattaac aggtctcttg aggtaattta tagaatattt
     1561 gtttacttaa tatcgtgaat tgctaaaacc caataaaagt gactgacatg tcatcaagaa
     1621 gacaacataa cagccacttt aatcagtaat taaactataa atataaaatc ggcgcagctc
     1681 aattgtcgat atctcacgcc attttaatat gcttatatat taaaccttaa acaagtgtaa
     1741 aagcttcatg taattttgaa agttctttct caagacgtaa cataatttgt ttgccatctt
     1801 cgcggtcaac taaacctaat ttaacagcaa aatcaacttc tttttgaagt ccaaacattt
     1861 gtgtatctag tacttcttca tataatgggc atgaaggtaa tgttaaatta tctagttgaa
     1921 ctttaatcag atgcaagatt tcatcagcat ctttagtcaa ttgtttcaaa gctgcatttt
     1981 tcattgttgc ttgtttcgcc atgacacgct ccccctctat ataaattctc aagtcttact
     2041 aaaagtttat cttactaaat attaaaaagc aagtagattt aaaatgtgtc gttagcaatt
     2101 tgtgttaaaa taatgctata tacattgata gggagtggga aaatgataca aattaaagga
     2161 gccgtaaagt ttcctataac attagatagt acgacttgga ttttcgatga tagaaaagtt
     2221 agcatcgaag atttagaaaa aggtgtattt gatggttcaa aaccaatcga ctttgaagat
     2281 aatcatgaat ggaatcgtgc tatcctagaa ggacaaacta atccgcctac tttaaattca
     2341 gaaataaaat ataaaaaacg tgccatgtta gaaggttcat ttgtcattaa tatgacacca
     2401 tttttcaaac attccgaacc acatgactct gcaactacta ttcgattatc aaatgagcat
     2461 gatcatttag atataccgat agatctatta ccctatttat ttttccaatt tgcaaaagac
     2521 ggtaaacgac tttacagcga taatggtgta gatagtttta tttacaatca agaagatggt
     2581 tattcatatc gaatcaacca tgtaactcat attgaggtga tttaatttga gacaagtgca
     2641 atgcattatc tgtgatgcaa aagtatttat agatgagcgc acaactgagt ctaaacgact
     2701 taaaaacaat cctattcgaa catttatgtg tgatgattgt aaaagtcgat tagacacacc
     2761 taaacaacgt gcgcaacact atccgctcga ttaatatctg aataaacaag ttagaaattt
     2821 gaaaatgtga aagtaacagg aaataatagc atttaaacac tttttcaaat attatatgcc
     2881 aaaagagaag ctaagcaact ttcatcgctc agcttctctt ttattatgtt aaattattcg
     2941 ttttcttgca tcatttgttt gatacgcttt gcttcttttt cacgaacatt tttgtttaaa
     3001 attttctttc ttaaacgtat actttctggt gtaacctcaa caagttcatc atcattaatg
     3061 aattgtaacg cttcttccaa tgttagaata cgcggtctat tcattgtttg tgtttggtct
     3121 ttcgttgcag aacgtacgtt agtttgatgt tttgttttag tgatgttaac agttaaatca
     3181 ttttcacgat tatgttcacc aacaatcata ccttcataaa cttcagtacc aggttccatg
     3241 aagtttacac ctctatcttc aagtcccaaa atggcataag tacttgcaga accttgatcc
     3301 attgaaatta atgcaccatt acgacgaccg ccaatttgtg ctttaatacg tggtctaaat
     3361 tcttcaaatg tatggttaat aataccgtaa cctcttgtca ttgacataaa ttcagtcgta
     3421 taaccaatca taccacgagc cggtacatta aagattaaac gtgtaagtcc attatcagtt
     3481 gtagtcatat caaccatttc acctttacgt gcacctaatg attcaataac agcacctgca
     3541 ttttcttgtg gcacttcaca ttgcacacgt tcaaatggtt cacacattac accatctatt
     3601 tctttaataa ttacttgtgg ttttgaaact tgtaattcat aaccttcacg acgcatattt
     3661 tcaataagga ttgataaatg caattcaccg cgaccagcaa ctacccatgt atctggagaa
     3721 tctgtgttag aaactttcaa agatacatct gtttctaatt gttgatttaa acgttcttga
     3781 atttgacgtg ctgttacaaa gtcaccttca cggccagcaa atggagaatt gttaacttta
     3841 aatgtcattt caagagtagg ctcatcaata cgtagaactg gcaatgcttc ttgatggtca
     3901 tgtggtgtta cagtttcacc aacattaatg tcttccatac ctgaaacagc aattaaatct
     3961 ccagcttgtg cttcttcaat ttctaaacgt tttaatccaa agtaaccaaa gattttagtt
     4021 acacggaagt ttttcactgt accgtctaat ttaattagtg atacattatc tccgacacgc
     4081 attttacctc tgaatacacg accaatacca atacgtccaa cataatcatt gtagtccaac
     4141 aatgctactt ggaattgtaa tggctcatca ctgttatcaa ttggagctgg tacataatca
     4201 ataattgttt catataatga ttgtaaatta tcatcttgct tttcaggatc taagctagct
     4261 gtaccattta ctgctgaagc ataaacaaca gggaattcta attgttcatc gtttgcttct
     4321 aattcaataa ataaatctaa aacttcatct acaacaccct ctggacgtgc tgatggttta
     4381 tcaattttat taacaacaac aacaggtttc aggttttgtt ctagcgcttt tttaagtaca
     4441 aaacgtgttt gaggcattgt accttcatac gcatctacta ctaagacaac cccatcaacc
     4501 attttcataa tacgttctac ttctccacca aagtctgcat gtcctggtgt atccaaaata
     4561 ttaatacgtg tacctttata atcaacagcc gtatttttgg ctagaatcgt aattccacgc
     4621 tctctttcga tatcgttaga gtccattgca cgttcatcga catgttcatt ttctctgaat
     4681 ataccagatt gttttaacaa ctcatctact aaagttgttt taccatggtc aacgtgagca
     4741 ataattgcta tattgcggac atcttctctt ttattagtca tataaatagt ttccttcttt
     4801 cttaagtaaa atcccacttt taattacaac ttttttatta tatcatataa ttgagaaaag
     4861 ataagcaaaa ggtggggata ggatgaaaca aaaaaaatct aaaaatatct tttgggtatt
     4921 ttctatatta gctgttgttt tcttagtatt atttagtttt gctgttggtg catcaaatgt
     4981 accaatgatg attttaacat ttatattact cgttgcaacc tttggaattg gatttactac
     5041 aaagaaaaaa tatcgagaaa acgattggct ataagttata tcgacaataa cacgcgttga
     5101 tttaagacca cacatgttca tgatgaacat ttaactggtt ttatattggc gcgtgttttt
     5161 attatttaag gtataaaagc gtaatatgtt acctaattgt taggactaca tattacgctt
     5221 ttggtaatta ttttgatttt cttttaaatc gttgttcatg taattgtatt aacgcatcat
     5281 ggtggggctc taaataatca ttgctaattt cttgatggag accacgattt ccaactaaga
     5341 ttgaatttgg accactaatt gttaatggtt ctcctagtaa attggaagct tgtccattta
     5401 cttcatataa aataaccaat ccgccagcaa aatcccatgg ttgaagtctt ggcgtcatat
     5461 atgcttctaa attacctgta gcaactgaaa cgatttcaag cgctgcacta ccatatgccc
     5521 ttgcacttct agaatcatta acaatttctt taaagatttc tcctaaaatt ggtttagtta
     5581 accagttcgg attgatccca ataatgcttt gtcttagatt agaatcattc aatggtttca
     5641 agggttggct accacgatat gcaccttccc ctactttagc atgatataag acatcagcca
     5701 taacatcata tacaaaacct gcataaggtt taccatcgat ataaatacca attgaaattg
     5761 cgaaattttc ttgttgatga acaaaattca atgtaccgtc tattgggtca acaatccata
     5821 ccgtaccttt ggaagtatcg atgtcatgac catgcccttc ttcacctaat acttgatgat
     5881 tgggatatgt ttctaaaatt gtatcaaaaa tgaaatcttc tgttgcttta tctacatttg
     5941 taacaaggtc attcggattt gactttgttt caattgttaa attttgctcc atcaattgtt
     6001 taattctaat tcctgcttct tgaataagtc cttgggcaaa tccataaagt gccataacat
     6061 catctcctaa catctttatt atacatcaac attttataaa aataacttct tatgataaaa
     6121 atgaaaatac taattttaat gagagcgtct taagccgcaa ttgataaaac atatgctaca
     6181 atatttttaa acactataca ggaggtactc tatgacaaaa tatacattta aacctaaaga
     6241 tttcaaagcg ttcaacgtag aaggcttaga cgcacgaatg gaagctttaa acgaatacat
     6301 acgaccacaa ctccgtgaat taggagaata ttttagtgac ttctttacaa gtcaaactgg
     6361 tgaaacattt tatcctcacg tagcaaagca tgctagaaga agtgtgaatc ctcctaaaga
     6421 tacatgggtt gcttttgcaa caaacaaaag aggctataaa atgttacctc atttccaaat
     6481 tggtatgttt gaagatcaac tgtttgttat gtttggaatc atgcatgaag caaaagataa
     6541 agcaacacgt gcaaaagttt ttgaaagaaa atttaaagct attcaacaat tacctgatga
     6601 ttatcgtgtt tgcttagatc atatgaaacc tgataaacca tttattaaag atttaacgga
     6661 tgatgattta atagaagcga tacaaagagc catcaatgtg aaaaaaggtg aattctttat
     6721 agcgcgtgca atcacaccac aagataaaag attaaaaagt gacaaagcat ttattgcatt
     6781 tttagaagaa accttcgatc agttcttacc attttattct gcataaataa ctttgtttaa
     6841 ataatagagc acttaatcac atccatgatt tcgtgctctt ttttcttaat attaaatcga
     6901 acgttcaaca taataattca tacttttaaa aaaattaaaa taaatttagg ttgacctaaa
     6961 cattttatta ggttattata ttgtccataa gaagtagagg tgagtcaaaa tgaataataa
     7021 acgacattca acaaatgaac aattaagttt agacgaaata aacaatacaa ttaaattcga
     7081 tcatcgcagt tcaaataaac agaaattttt atcatttctt ggacctgggt tattagtcgc
     7141 tgttggttac atggatcccg gaaactggat aacatcaatg caaggtggcg cccaatatgg
     7201 ctatactttg ctattcgtaa ttcttatttc aagtttatca gcaatgttac ttcaaagcat
     7261 gacagtgaga ttaggaatag caacaggtat ggacttagct caaatgacac gacattattt
     7321 atcaagacct attgctataa tcttttggat cattgcagaa ctagcaatta tcgctacaga
     7381 tattgctgag gttattggta gtgctattgc tcttaatctc ctatttaaca tacctttaat
     7441 cgtcggtgca ctaataactg tacttgatgt atttttacta ctttttataa tgaaatatgg
     7501 ttttagaaaa attgaagcta ttgttggtac attaattttc acagtgttat tcatctttat
     7561 atttgaagtc tatatttcat caccacagtt gaatgctgtg ttaaatggat ttataccaca
     7621 tagtgaaatc attacaaata acggcattct ctatattgca ttaggtatta ttggcgctac
     7681 aattatgcct cataatttgt acttacattc atcaattgta caatctagaa catactcaag
     7741 acataacaat gaagaaaaag cgcaagcgat taaatttgct acgatagatt cgaacattca
     7801 gttatcaatc gcatttgtag tcaattgctt attattagtg ttaggagcat cactattttt
     7861 caactcaaat gctgacgatt taggtggttt ctatgattta tatcacgcct taaaaactga
     7921 acctgtacta ggtgcaacaa tgggtgcaat catgagtaca ttatttgcag ttgcattatt
     7981 agcgtcaggt caaaattcaa cgattactgg tactttagca ggacaaattg taatggaagg
     8041 atttttaaga ttacacatac caaattggtt aagacgttta attacacgtt ctcttgctgt
     8101 cattcctgtt atcgtatgct taatcatttt taaaggtaat gcagctaaaa ttgagcaact
     8161 acttgtattc tctcaagtat ttttaagtat tgctctgcca ttctgtttaa taccattaca
     8221 attggctact agtaataaag acttgatggg acctttctat aataaaacat gggttaatat
     8281 catttcatgg acacttatta ttattctaag tattttaaat gtctatttaa tcgttcaaac
     8341 attccaagaa ctacaaagtt aaaaataaaa ccattaaaat gttggttgta tctaataatt
     8401 aacaacttaa cattttaatg gtttttcttt tgctatgaac atattaatga taaatatact
     8461 catcgtcctt atgtgttgtt tcttttgatt cttttttaga aatcaacata atcatcacga
     8521 taacccataa gacaattgca attaaattaa gagccatcaa acttacaatt atcgcgataa
     8581 caaaaagtat aatcgataat tttctacgtc ctttaataca aattgtcgca aatataccat
     8641 aaagtgttga aattaataaa ataattgcta atgcacccat tgttgtctga agcatatcta
     8701 aagaaacctt gccaccgttc tgagataatt gcttcgctaa ttcttcacgt tgtgatgcat
     8761 taccactttt taaagaaaaa taggatagcc ccataactaa taaataaata atactaatac
     8821 tatttgcaat ccaagccatg attaattcag gttttctatt catcttaaaa tcctttcttt
     8881 gtttaatatt ttaataagta tactacgagc ctatagatat acgcacatta aattacttgt
     8941 atattaacat atttttcatc taatttttat aataaacatc tgaattcaga ttgatgataa
     9001 tttaggtgta tagtaatgag aaatcgtcat ttaaaattat gacacctaaa cactttaatt
     9061 aaacgttatc gataattaac ctcatataaa aacccagatt aataagcaaa agacctctgc
     9121 ttactaatct gggtatcatc aatatttatt cttgctcttt tttgtcctta acttcacttg
     9181 aattgcgttc ttcagaaata tcattgttgt aaatttgacg tcgctcttca aaattcattc
     9241 gacgttgact aactgcgcta ggttgatttt tcctacgttc tttcattcgt tggttataag
     9301 ctttacgttt ttcttttttc ttagctttaa tttcagcttt ttctcgcttt tgttgtgctt
     9361 tcaattcttt tggatcaact tcaggttctg cataataacc atcatcttca atacctagtt
     9421 cttcagtact tttttgtttg tatttagcct gtctggataa tactgatggt tcttcctcag
     9481 tgcgctcatc atgattatat tcactttcaa ctgcacgttt aggaaattga tcatatttat
     9541 ctctttcctg ttcttgatca tatacagtat ttgatttatc caattctttt tcatatttag
     9601 gcacatcata ataatcatct ttcggttggg ggcgctcata cacaggttca cgataatcat
     9661 aaatcggtcc gttatactct tcataatatt gtggcgcaac catttcaact ttatcttttc
     9721 ttataaatag catcatcgca ataataaaga ataatatcgg aatgattaaa gtaacgataa
     9781 gtaaaactaa aggaatcgtt acaattgctg atattaagaa taagaaacct gacaagattc
     9841 ttatattcat agatatgaga gctaaaaatg aaatcaacaa acaaacaata aagtaaacga
     9901 taatagccca tataccattt tgtaaccata ttacaaattg tgttgtatta tagccatttc
     9961 cagctaataa ttgctggata aatgcattat tgtttaatgt attttcaaga ttagcaatcg
    10021 atgtgttatt actgaatgaa acaagtgcta taaacatcgt aatgacagta agcactaata
    10081 acatcaccca acataaccaa cctagaactt tttcagttaa tctacttact ggacgtttaa
    10141 tttgagtaaa ttgttctcca gtcattcgtt acaactcctt atagtactta tcccgttatt
    10201 ataactaaat atacagtaaa taactactat ttatgatttt attttaatga cattttgaaa
    10261 ttcaaaaagt tttcattgta ttcacttaaa acttcaggtc ctaaatcttt ataaacttca
    10321 aggcgttctt gctctttctt agtcggataa aaacgatggt cgtctttaat ctctttaggc
    10381 aacaattgtc gagcagcctt gtttggcgtt gcatagccta cgaattctgt attttgcttg
    10441 ttatttttag catctaataa aaaattcata aatttatatg caccctcttt attttgtgcc
    10501 gtttttggaa ttaccatatt gtcgaaccat aaattcgatc cttctttagg aataacataa
    10561 ttatatttat ccccttcttg cactagaggt gctgcaacac cactccaaac aaccgctatg
    10621 ttaccttcat tttgttgaag catcatggta atttcatcac ctacgacacc tcttacttgt
    10681 ggtgctagtt tggttaaatc tcgctctgct tcttttaaat ggtgcgaatt acggtcatta
    10741 agattatacc caagtttatt caaactcatg cctataatct ctctagcacc gtcaactagt
    10801 aaaatttggt ttttaaattt aggattatac aatgacttcc aactatcaaa tgattcattt
    10861 ggatactttt ctttattata taaaatacct acagttccaa agaaataagg taaagaatat
    10921 ttattgcctc tatcaaatga catattcata taatctgaat ctaaattttt aatattaggt
    10981 accttattat gatctattgg taacaataaa tgatctcttt tcaatttttg aactgtatat
    11041 tcactaggaa aagcaacatc ataatgtgta ccgccattgc gaattttggc ttccatcgct
    11101 tcatttgaat cgaaagtttc ataaacgact tgaatgccag tttctttttc aaatttctta
    11161 attaactctg gatcaatata ttcgccccaa ttgtatacgt aaattttttg atttgtatgc
    11221 acttgttctt tagatttaaa ccaatgactt aaagtaagac aaagcatacc cacaactaat
    11281 gcacctataa tgagttgtaa aaatcgtttc attattttac acctcgcttg attagttttt
    11341 tcttatttat cacgtattga atcaaataat atcctagtat tcctaataca ataacagcaa
    11401 acaataatgt tgaaatcgca ttaatttcca tactaattcc ttttctcgcc atagcataaa
    11461 cttcaactga taacacacta aagccattac cagtaacgaa gaaacttact gtgaaatcgt
    11521 ctagtgaata agttaaagcc ataaagaatc ctcctataat agaaggtaaa atattaggaa
    11581 taataatgtt gcttaataat tgtggttcag tcgctcctaa atctcttgca gcatttaaca
    11641 tattattatt catttcatac agttgtggta agacgataat cacaactata ggtatgcaaa
    11701 atgcaatatg agatattaga actgtccaaa atcctaaacc aagaccagta aaatggccaa
    11761 tcgttgtaaa cataattaag aatgatgcac ctatgacaac gtcggatgat accatcaaga
    11821 cattattcaa tgttagtaaa gttactttaa actttttatt tcttaaataa taaatagcaa
    11881 tggcaccaaa tgtaccaata actgtagaaa ttgaggctgc taaaagtgct acagctatcg
    11941 tattaaaaat gaccgacatt aaatgatcat tttgaaataa tgattgataa tgctctaatg
    12001 taaaatgttc aaagtgaatc atattaccag ccgaattgaa tgaatagaac attaaaaaga
    12061 atattgggat gtataaaatc gctaaaagta tcccgatata cagctttcca taccatttca
    12121 tatgattcac cctctcccat tagatgattt tgtaatgatt aaaatgaatg ccataaatac
    12181 aattaagaat atagctatag ttgatcccat accataattt tgaattgtta aaaattgttc
    12241 ctctattgcc gtacctatat ttatgacttt attacctgca attaatcttg taatcataaa
    12301 taatgaaagt gatggaataa aggttacttg aatcccagtc ataacacctt cttttgttaa
    12361 cggcatgatt acttttctaa aagtatagaa aggactggca cctaaatcac ttgaggcctg
    12421 caataaatta ttaggaattg ctttcatgct attaaatata ggtaaaatca taaatggtat
    12481 ataaatgtaa cttgccacta ctaaaaacgc accagttgta aataacaaat tgaatgatgg
    12541 taaattaaat aagtggaaaa attgattaat cacgccatca tgacttaata aacctataaa
    12601 agcatatgtc tttaacaata aatttatcca tgttggaata atcattatca ttaataagat
    12661 attttgaaat ttcgaacgag taatataata ggcagctgga taactgatag tcaaggtaat
    12721 aattgttatt gaagcggcat ataaaattga atatgcaaac attttcaaat attttgtagt
    12781 aaaaatttgt tgataattcg tgaaactaaa atgtccattg atatctaaaa atgaaaaata
    12841 aatgagtaat acaactggta caataataaa tataaccatc catagtaaat acgggattaa
    12901 gagaaattta ttagtattac gcattgtcta cttcctcata gctttcaata cgtttatcaa
    12961 attcttcttc tgtttctcca ggaaccataa tatgaatggc ttcaggatca aaataaagac
    13021 caacttcact gcctacttca gcttttttag tcgtttgtat tacccattca taacctttat
    13081 tgtctataca acatatttca tagtggaccc ctctaaataa catagaatca acagttgctt
    13141 taaataatcc ttcttcagct ttgattaatg atatatcttc tggtcgaata acgacttcta
    13201 cttttttatt ttcaggaata cccatatcga cacattcgaa atcttgccca taaatattca
    13261 cgacataatc tctaaccatg cgcccttcaa caatattaga ttctccaata aaatcagcta
    13321 caaatcgatt cactggttcg tcatatatat ctgttggtgt gccaaattgt tgaattttcc
    13381 catcttttaa tacaaaaaga aagtcactta atgctaacgc ttcttcttga tcatgtgtta
    13441 caaatataaa tgtaatacct aatctagatt gcaattctcg taattcatat tgcatttcag
    13501 tacgcaattt caaatctaat gcggataaag attcatctaa caataatatt tctggttcat
    13561 ttacgatagc acgtgcaatt gcaacacgtt gcttttgtcc gccactcatt tcattaatat
    13621 ttcttttttc ataacctgaa agttttacta attttaatgc ctcagttact ttttgatcaa
    13681 tttcggtttt tgataatttt tttaatttca aaccaaaagc gatattatca tagacgttta
    13741 agtgtggaaa taatgcataa tcttgaaaga ctgtattcac tttacgttta tttggtggta
    13801 aattaccaat tggtttgttt tgataaatca cttcaccact gtcaggatat tcaaaccctg
    13861 caattaattt taaaattgtt gttttaccac aacctgaagg acctaataat gtataaaagt
    13921 atcctgattc aatatcaata tctatgtcat ctaagatatt aagatcatca tagcttttac
    13981 taactgattt taatgataat aacggttcca aataagatcc cccctataaa tatgaagctg
    14041 tcgctacaat aagtattcgt gtttcattgt ttgattcgtt tgacaaacga tgattatctc
    14101 gtgctttaaa atacaaaacg tcttcttctt gtgcttgata tatctctttg cctaaattaa
    14161 gtgttatctg acctgacata caataaataa acgtatcaga ctctgatgga ttaaaatttt
    14221 tatatgatgc tccaggcttt aaagttaata ttaatggctc catatcatat tcatttgact
    14281 ttgaaactaa ccaatttaat atataacctt catcatactc atcataaata acttgttctt
    14341 ccttcttgta taatactttt tcattttcac tgtctttaaa aaattcactt ggcgtcgttc
    14401 ctaacacctc tataatattt aagaaagttt ccatacttgg tgaggcatgt tcactttcta
    14461 tttgtgaaat gtagcctttc gataagtctg tacgttcagc aagttcttct tgcgttaaat
    14521 ttttaattct tctaagattt ttaattttat tacctatgtt catttatttt tccacctaaa
    14581 aaagctatat taagtttact tttatcaaac attttgttta acacttgata aaggtatcaa
    14641 aatatagctt tattagcaat agttttaaag aaaaattcct attaaattgt gttaatttcg
    14701 aacgaattat tttttagctg ttactctttg ttcttcagag tgagttttta catcttgaac
    14761 tgctttgtaa ctattatagc cactactttt ttcgaaagta ttaaaaattt gtttttcctc
    14821 tgctttagca ggcacaattt ctttaaatct tttatatgca cccataaagt cgcctgccgt
    14881 cacgccggat tcataatact tctctacatg attaaagaaa tttatcactg aaatcatctc
    14941 ttcattactc cagtctaaat caattggata ctcatattcc attgttgaac cctccatttc
    15001 aattcttttg ttagcatcgc tagtataaca tgcatgtcac atgttaacga tatctcaaat
    15061 caatttcctt acattacttt acctaaaaca aaaaagccgt agcatatgct acagctttta
    15121 atgactgaat ctttatagat aatcagttat tacattgtat ggattgggta tccgatagct
    15181 ttttctgctg cttccatagt catctcacct aatgttggat gtgcatggat tgttaatgcg
    15241 atatcttcag cattcatacc agcttcaatt gctaaaccta attcagagat aatatctgat
    15301 gcaccagtac caactacttg agcaccgatt aaagtatcat cttctttaag tgtaataagt
    15361 ttaacaaatc cgttagtatc atctaatgat aatgcacgac catttgctgc atatgggaat
    15421 ttagaagctt taattgctaa accttcttct ttagcttgcg cttctgaata accaactgta
    15481 gctaattctg gttcagtaaa gcatactgct ggcataccaa tgtaatcaac ttcagcagct
    15541 tgaccatcaa ttgcttcagc agcaacttta gcttcatagc tagctttgtg agcaagtggt
    15601 aaacctggaa cgatatcacc aattgcatag atattgctga tagacgtacg gctttgttta
    15661 tcaacttcta ataatccacg gtcagcgaat ttaacaccta attcttctag gcctaattcg
    15721 tctgtgtttg gacgacgacc tacagttact aatacataat cagcttcgat tgttttctct
    15781 tcgcctttag cttcataagt aactttaact ccgttatctg tttcttcagc tgatttagcc
    15841 atagcttcag taacgatttc aacacctttt tctttcatac cttttttaac aggttgtgtc
    15901 atttgttttt cgaagccacc taagatatct ttagcacctt caaggatggt tacttctgaa
    15961 ccaaagttag caaatgctgt acctaattct gatccaatgt atcctccacc aactacaact
    16021 aatttacctg gtacttcttg taagtttaaa gcacctgttg agtcgataac acgtttaccg
    16081 aatttgaaat taggaatttc aattggtctt gaacctgttg caatgattcc atttttaaag
    16141 ttgtatgttt gtgcgctctt ttcgtccata acacgtaagc tattgttatc tacgaaatat
    16201 gcttcacctt taacgatgtt aactttgtta cctttaagta agccttcaac accaccagtt
    16261 aatttattaa caactgatga tttgaattct tgaacttttt ggaagtttaa agaaacactt
    16321 tcagcaataa cacctaagtt ttcagaatgt tgtgcttcaa caaaacggtg agaagcatgt
    16381 agtaatgctt ttgaaggaat acatcctacg tttaagcaaa caccaccaag attacctttc
    16441 tcaacgattg ttactttttg tcctaattga gctgcacgaa ttgctgcaac gtatccacca
    16501 ggacctgctc cgattactat agtatctgtt tcaattggga aatctccaac taccatgttt
    16561 tacccctcca ttaataataa ttctggatta tttaataaac gtttaatgtg attcattgca
    16621 ttttggccag ttgcaccatc aatttgtctg tggtcaaagc ttaatgataa tgctaatact
    16681 ggtgctgcaa caatttctcc atctttaacg ataggttttt gagcaatacg gccaattcct
    16741 aagattgcta cttctgggtg attgataact ggagtgaacc attgtccacc agctgaaccg
    16801 atattactga ttgtgcatgt agcacctttc atttcatcgg ctgttaattt accatcacgt
    16861 gctttaacag ctaattcatt aatttcatct gaaatttgga aaatagactt acgatcagca
    16921 tgtttaacaa caggtactaa taatcctcta tcagtgtctg ctgcaatacc gatattccag
    16981 taatgtttat gaacgatttc accagcttct tcattgaatg aagtgttaag tgctgggtat
    17041 tttttcaatg cagaaacaag tgctttaaca acataaggta agaatgttaa cttagtacct
    17101 tgttcagctg cgatttcttt aaatttctta cggtgatccc ataatgcttg aacatcaatt
    17161 tcatccatta atgttacatg aggtgcagta tgcttagagt taaccattgc tttcgcaatt
    17221 gctctacgca tagcagggat tttttcagtt gtttctggga agtcgccttc taatgttact
    17281 gctgcaggtg ctgcaggagt ttcagcaact tcttcacttg tagctgaagc agctgattca
    17341 tttgaagctg ttggtgcacc accatttaag tatgcatcta catcttcttt tgtaatacga
    17401 ccatttttac cagatccaga aactgcttta atgttaacac ctttttcacg tgcgtattta
    17461 cgtactgaag gcattgcttt aacagttctg ttttcatcta cttcttcagt ttgagtagct
    17521 acaggtgctt gctctgctgg cgcttcctct ttcgcaggtt cttctttaga tgatgaatca
    17581 tcatcatgac ctttaaattg catatcttct gcatcaggtg catcgatttt aacaataacg
    17641 tcaccaacta cagctactgt accttcttct accataactt cttctacagt accagatact
    17701 ggtgatggga tttctactac tgatttatcg ttttgtacct cagctaaaac atcgtcttct
    17761 tcaatagtat ctccagcttt aacaaaccat tttacaattt caccttcgtg gataccttcc
    17821 ccgatatcgg gtaatctaaa ttcaaatgcc acgtttttgc cctcctaaga tttcgtaaat
    17881 taaacatttc attttaatca atgagactaa aatacgctaa cttcgttaac ttttaaaatg
    17941 tattaaaatt ctaaagtttc ttttgctttt tcgatgatgt catttttgtt tggtaaccaa
    18001 acattttcag cttgagtgaa tggataaatt gtatctgctg ctgcaactct tccaataggt
    18061 gcttctaatg aaaggattgc acgttcactt aattcagcta caactgctgc accaacacca
    18121 gcttgacgtt gtgcttcttg aactacaact gcacgaccag ttttttcaac tgaagctaca
    18181 attgtgtcaa catcgattgg ttgaacagta cgtaagtcaa ttacttcaac agaataacca
    18241 tctttttcaa gttcttctgc agctttcatt gattcttgaa ccatcgcacc gtatgtgatg
    18301 attgaaatgt cattaccttc ttttttcaca ttagccttac caatgtcaat tgtatattct
    18361 tcttcaggta cttcttcacg gaatgaacga tacaatttca tatgctctaa gtatacgact
    18421 gggtcattac ttctaataga agaaattaat aaacctttcg catcgtatgg gcctgaagga
    18481 ataacaacct ttagacctgg agattgagct aaaatacctt ctaagttatc tgcgtgtaat
    18541 tctggtgtgt gtacgccacc accaaatggg ctacgaattg ttacaggtgc agttttagta
    18601 ccgcctgaac ggaaacgagt acgtgcaatt tgtccagcaa tcgcatcaaa tacttcgaat
    18661 acgaaaccta agaattgtac ttccataacc ggacggaatc cttcaactgc aagacccatc
    18721 gctaaaccac caatacctga ttcagctaaa ggtgtatcga atactctatc ttcaccaaat
    18781 tctttttgta gtccttcagt aacacggaaa acaccgccgt taacaccaac gtcttcacca
    18841 aaaattaaaa catcttggtc atttttaagt tcagttttaa gcgcatcatt aatcgcttga
    18901 accattgtca tttgtgccat ggcttacttc gactccttct ctttgtaaat ttcatattgt
    18961 tctgctaagt tttgaggcat atcttcatac ataatttcca ttagagaagt aacagtttgt
    19021 ttttcagtgt tatcagcctc tttaattgct gctttaatat cagcttttgc acgttcaata
    19081 acttcatttt ctttgtcttc attccataaa cctttgtttt caaggaattt acggaaacgt
    19141 actaatgggt cttttttctc ccattcagca tcttcgtctg aagttctgta acgagttgga
    19201 tcgtcaccag ccattgtatg aggaccataa cgatatgtca tagtttcaat taatgttgga
    19261 ccttcacctg caactgcgcg gtcacgtgct tctttagttg cttgatatac agctaacgca
    19321 tccataccat caacttggat accaggaata cctacagcaa ttgctttttg agctaatgtt
    19381 tcagcagcag tttgcttgct tcttggtgtt gaaattgcat agttattgtt ttgaataacg
    19441 aaaattgcag gtgctttata agctgctgca aagttaatac cttcgtagaa atcaccttgt
    19501 gaagaaccac cgtcaccagt gtaagtgatt gcaactgcat ttttaccacg ttttttaagt
    19561 gcaaatgcaa caccagcagc ttgaatgtat tgtgcaccga taataatttg tgggcttaat
    19621 gcattaacgc cttcagggaa ttgatttcct ttgaagtgac ctcttgagaa taagaaagct
    19681 tcagttaatg gtaaaccatg ccaaataatt tgaggaacat ctctgtatcc cggtaaaatg
    19741 taatcttctt tttctaaagc gtattgtgac gctaattgtg atgcttcttg accagcagtt
    19801 ggtgcataga aacctaaacg tccttgtctg tttaatgaga tagaacgttg atcaaggata
    19861 cgagtccata ccattctttc cattaattcc actaattgtt catccgtaag atcaggtact
    19921 aagtcttcat ttacgacgtt accattctca tccaaaattt gaaccatttc aaatttcgat
    19981 tgagtatcat ttaaaacttt tactgcatcg aattgggctt gtaacttagg agccattcaa
    20041 ttcaccatac ctttcccata attgtataga aattcatttt atccttacta attgtaccac
    20101 aaataaatgg gactgttaaa ccatttgaaa aagttctgtt ttaataaaaa aagatacaga
    20161 taataggaac tgtattagtt gtttcctacc atctgtatta tattaattat ttaaattgat
    20221 caacgtcttg cttttcttta ccaaccttat ttagtacttt tgtatactta tctgatactt
    20281 cttttaactt tttatagttc tgttctattg cttttgattt ttcgttaaca ccttgttgtg
    20341 tcgcgtcatt ttgatttaaa tatttaaata atgttttctc tgagtttaca gcctttttat
    20401 atgctttcgc gtaatcactg tgtaacttat atttttcttt taatacatca tctaattgtt
    20461 ttacttcttt acgtttaaca tcattatcaa tgttatcaac gtgacttttt gctttcttaa
    20521 agtcttgttc agacttctta attgcgtctt cttctttttc aaattccttt agacgatcat
    20581 cggcattttt aattaaatcc tttactgctt taccgcgaac tgctgtatct ttactattga
    20641 catcttcagt taattttttc tttttctcat ctaattcagc aattttctta ccaacagttt
    20701 taattggttc ttcatcatct ttaattttat ccacttgctt taaatatgcc ttaatttctt
    20761 ttttatccgt agtacatcct gcaagcaaga cactagatgc taatactact gcgattgttt
    20821 ttccaaattt cattacttgt acctccactt gctatttaaa ttaaatgata aataaaatct
    20881 gcttggaaat caattttaaa caattacatt acacatcttc attactaagt atatctgttg
    20941 acagttacgt catttataat ataattttta aagtcaagat gataaataaa tttctaaagt
    21001 atcattgctt gaaaccaaca ctttaatcat tttttaagca attagcatat ctgaaaattt
    21061 acatatacta tctaaaataa acattggcag tagttggtta actttgtaga tatttacaca
    21121 taaaaatgta tgagtatctt aacaaccttt ttactgtcat tcaactaaaa caaattaaaa
    21181 taaggaaggt gcaatatatg ttaacaatga aagacatcat tagagatggt catccaactt
    21241 tgcgtcaaaa agcagctgag ttagaattac cattaactaa agaagaaaaa gaaacattaa
    21301 tcgccatgag agagttttta gtaaatagtc aagatgagga aatcgcgaaa cgatatggtt
    21361 tacgttcagg cgttggtttg gctgcacctc aaattaatat ttctaaacgt atgattgctg
    21421 ttttaatacc agatgatggc agtggcaaat cttatgacta tatgcttgtg aacccaaaaa
    21481 ttgtaagtca tagcgttcaa gaagcttatt taccaactgg tgaaggttgc cttagtgtcg
    21541 atgataatgt tgctggtcta gttcaccgtc ataatagaat tacaattaaa gccaaagaca
    21601 tcgaaggtaa tgatatacaa ttacgactaa aaggatatcc agcaattgtt ttccaacatg
    21661 aaattgacca tttaaatggt gtaatgttct atgatcacat tgacaaagat cacccattac
    21721 aaccacatac agatgcagta gaagtttaaa acacattttt ctaattatca aagcttagga
    21781 taaatatgat gtcctaagct tttcctttta caactttttc gaataaacaa cagttaaata
    21841 tattcacctt tctaccaaac tttttatccc ctcatttaaa ttttaccgtt tcatataaaa
    21901 tcctttaatt ctttcttaac attaatttat aatctctaca tttattttaa taaatagaac
    21961 tgcacattta ttcgaaatac ttagatttct agtgagataa actgctttat ttattatcat
    22021 tcatcatgta aaataagatt taactgaaat tttagtgtta tttcactaat tttttaaaat
    22081 gaacgacatg atgaacctag ttattaacca aatcgttatt aagttacatt atagagatga
    22141 ttggaatgaa tttatcgata tatactccaa tacgatttta ctagggttaa caataaatta
    22201 aacaaacatt cttaggagga atttttaaca tggcagtatt taaagttttt tatcaacata
    22261 acagagacga ggtaattgtg cgtgaaaata cacaatcact ttatgttgaa gctcaaacag
    22321 aagaacaagt acgtcgttac ttgaaagatc gtaattttaa tatcgaattt atcactaaat
    22381 tagagggcgc acatttagat tacgaaaaag aaaactcaga acactttaat gtggagattg
    22441 ctaaataatg aaacaattac atccaaatga agtaggtgta tatgcacttg gaggtctagg
    22501 tgaaatcggt aaaaatactt atgcagttga gtataaagac gaaattgtca ttatcgatgc
    22561 aggtatcaaa ttccctgatg ataacttatt agggattgat tatgttatac ctgactacac
    22621 atatctagtt caaaaccaag ataaaattgt tggcctattt ataacacatg gtcacgaaga
    22681 ccatataggc ggtgtgccct tcctattaaa acaacttaat atacctattt atggtggtcc
    22741 tttagcatta ggtttaatcc gtaataaact tgaagaacat catttattac gtactgctaa
    22801 actaaatgaa atcaatgagg acagtgtgat taaatctaag cactttacga tttctttcta
    22861 cttaactaca catagtattc ctgaaactta tggcgtcatc gtagatacac ctgaaggaaa
    22921 agtagttcat accggtgact ttaaatttga ttttacacct gtaggcaaac cagcaaacat
    22981 tgctaaaatg gctcaattag gcgaagaagg cgttctatgt ttactttcag actcaacaaa
    23041 ttcacttgtg cctgatttta ctttaagcga acgtgaagtt ggtcaaaacg tagataagat
    23101 cttccgtaat tgtaaaggtc gtattatatt tgctaccttc gcttctaata tttaccgagt
    23161 tcaacaagca gttgaagctg ctatcaaaaa taaccgtaaa attgttacgt tcggtcgttc
    23221 gatggaaaac aatattaaaa taggtatgga acttggttat attaaagcac cacctgaaac
    23281 atttattgaa cctaataaaa ttaataccgt accgaagcat gagttattga tactatgtac
    23341 tggttcacaa ggtgaaccaa tggcagcatt atctagaatt gctaatggta ctcataagca
    23401 aattaaaatt atacctgaag ataccgttgt atttagttca tcacctatcc caggtaatac
    23461 aaaaagtatt aacagaacta ttaattcctt gtataaagct ggtgcagatg ttatccatag
    23521 caagatttct aacatccata cttcagggca tggttctcaa ggtgatcaac aattaatgct
    23581 tcgattaatc aagccgaaat atttcttacc tattcatggt gaataccgta tgttaaaagc
    23641 acatggtgag actggtgttg aatgcggcgt tgaagaagat aatgtcttca tctttgatat
    23701 tggagatgtc ttagctttaa cacacgattc agcacgtaaa gctggtcgca ttccatctgg
    23761 taatgtactt gttgatggta gtggtatcgg tgatatcggt aatgttgtaa taagagaccg
    23821 taagctatta tctgaagaag gtttagttat cgttgttgtt agtattgatt ttaatacaaa
    23881 taaattactt tctggtccag acattatttc tcgaggattt gtatatatga gggaatcagg
    23941 tcaattaatt tatgatgcac aacgcaaaat caaaactgat gttattagta agttaaatca
    24001 aaataaagat attcaatggc atcagattaa atcttctatc attgaaacat tacaacctta
    24061 tttatttgaa aaaacagcta gaaaaccaat gattttacca gtcattatga aggtaaacga
    24121 acaaaaagaa tcaaacaata aataatcaaa aagctactaa ctttgaagtg aagttttaat
    24181 taaactcacc acccattgtt agtagctttt tctttatata tg
//
LOCUS       DALAGD010000038        24009 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.038, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000038 DALAGD010000000
VERSION     DALAGD010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 24009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24009
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.038"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            141..596
                     /locus_tag="O2701_001231"
     CDS             141..596
                     /locus_tag="O2701_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCZ4293134.1"
                     /translation="MVKHLQDHIQFLEQFINNVNALTAKMLKDLQNEYEISLEQSNVL
                     GMLNKEPLTISEITQRQGVNKAAVSRRIKKLIDAKLVKLDKPNLNIDQRLKFITLTDK
                     GRAYLKERNAIMTDIAQDITNDLNSEDIENVRQVLEVINHRIKTYSNHK"
     gene            836..2218
                     /locus_tag="O2701_001232"
     CDS             836..2218
                     /locus_tag="O2701_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293135.1"
                     /translation="MKSCPKCGQQAQDDVQICTQCGHKFDSRQALYRKSTDEDIQTNN
                     IKMRKMVPWAIGFFILILIIILFFLLRNFNSPEAQTKILVNAIENNDKQKVATLLSTK
                     DNKVDSEEAKVYINYIKDEVGLKQFVSDLKNTVHKLNKSKTSVASYIQTRSGQNILRV
                     SKNGTRYIFFDNMSFTAPTKQPIVKPKEKTKYEFKSGGKKKMVIAEANKVTPIGNFIP
                     GTYRIPAMKSTENGDFAGHLKFDFRQSNSETVDVTEDFEEANISVTLKGDTKLNDSSK
                     KVTINDHEMAFSSSKTYGPYPQNKDITISASGKAKDKTFTTQTKTIKASDLKYNTEIT
                     LNFDSEDIEDYVEKKEKEENSLKNKLIEFFAGYSLANNAAFNQSDFDFVSSYIKKGSS
                     FYDDVKKRVSKGSLMMISSPQIIDAEKHGDKITATVRLINENGKQVDKEYELEQGSQD
                     RLQLIKTSEK"
     gene            2249..2377
                     /locus_tag="O2701_001233"
     CDS             2249..2377
                     /locus_tag="O2701_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293136.1"
                     /translation="MEYKQHPFKVFTISVNYFEGIVFCGKIYEVLRFISFYTFYKL"
     gene            2487..3695
                     /locus_tag="O2701_001234"
     CDS             2487..3695
                     /locus_tag="O2701_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HCZ4293137.1"
                     /translation="MNDLTHFNKRSPIFIIILGSLTAIGALSIDMFLPGLPDIRHDFQ
                     TTTSNAQLTLSMFMIGLAFGNLFAGPISDSTGRRKPLIIAMIIFTLASLGIVFVHNIW
                     LMVALRFLQGVTGGAAAVISRAIASDMYSGNELTKFMALLMLVNGIAPVVAPTIGGII
                     LNYSVWRMVFVILTIFGFVMVIGSLLKVPESLTVTNRESSSGLKTMFKNFKILLKTPR
                     FVLPMLIQGMTFVILFTYISASPFIIQKIYGMTAIQFSWMFAGIGITLIISSQLTGYL
                     VDFIDSQKLMRGMTMIQIIGVILVTIVLLNHWNFWILAIGFIILIAPVTGVATLGFTI
                     AMDESSSGRGSSSSLLGLVQFLFGGVASPLVGVKGEDNPIPYIIIIIATAVILIILQI
                     YNMKVFKTNR"
     gene            complement(3925..4476)
                     /locus_tag="O2701_001235"
     CDS             complement(3925..4476)
                     /locus_tag="O2701_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCZ4293138.1"
                     /translation="MKRQAKIEIQNALVDLMAEYPFQEISTKMICAYCNINRSTFYDY
                     YKDKFDLLDTINSKHKEKFQFLLSALHHNFENIKQDKLKLYKFFIIIAKYIKHNEQFF
                     KDILVTYPMKTLFIDYINLARDYYQQIMNDYSTTVTNKQLYVTYIIGGQAGVFINWLS
                     NGCNESPEEVADILLANTIKLQN"
     gene            4597..5244
                     /locus_tag="O2701_001236"
     CDS             4597..5244
                     /locus_tag="O2701_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCZ4293139.1"
                     /translation="MKKMILINVITVVVLLAIGIAGFYFWNKTTSYVTTDNAKVNGDQ
                     IKIASPASGQIKSLNVKQGDKLDKGDKVAIVTVQGQDGETKDMDLKMPQKGTIAKLDG
                     MEGSMVQAGNPIAYAYNLDDLYVTANIDEKDIKDVEVGKDVDVTIDGQKASIKGKVDS
                     IGKATAASFSLMPSSNSDGNYTKVSQVIPVKITLESEPSKQVVPGMNAEVKIHKN"
     gene            5257..7188
                     /locus_tag="O2701_001237"
     CDS             5257..7188
                     /locus_tag="O2701_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCZ4293140.1"
                     /translation="MTTTFIISYIILALIIVGVINLFLIRSRKKGKRQQKEQQFTTRQ
                     SNQSKFKASDLDKTTDQSTQRMTHEELRVDNQDDHSQVSLNGYTKGSEKDQEAFTNNK
                     DEEAVAAKNPESEEYKVNEKIKKEHKNFIFGEGVSRGKILAALLFGMFIAILNQTLLN
                     VALPKINTEFNISASTGQWLMTGFMLVNGILIPITAYLFNKYSYRKLFLVALVLFTIG
                     SLICAISMNFPIMMVGRVLQAIGAGVLMPLGSIVIITIYPPEKRGAAMGTMGIAMILA
                     PAIGPTLSGYIVQNYHWNVMFYGMFIIGIIAILIGFVWFKLYQYTTNPKADIPGIIFS
                     TIGFGALLYGFSEAGNKGWGSVEIETMFAIGIIFIILFVIRELRMKSPMLNLEVLKFP
                     TFTLTTIINMVVMLSLYGGMILLPIYLQNLRGFSALDSGLLLLPGSLIMGLLGPFAGK
                     LLDTIGLKPLAIFGIAVMTYATWELTKLNMDTPYMTIMGIYVLRSFGMAFIMMPMVTA
                     AINALPGRLASHGNAFLNTMRQLAGSIGTAILVTVMTTQTTQHLSAFGEELDKTNPVV
                     QDHMHELASQYGGQEGAMKVLLQFVNKLATVEGINDAFIVATIFSIIALILCLFLQSN
                     KKAKATAQKLDADNSINHE"
     gene            complement(7434..8072)
                     /locus_tag="O2701_001238"
     CDS             complement(7434..8072)
                     /locus_tag="O2701_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293141.1"
                     /translation="MTNPNQRLEPFDETFQQPNIHRGKRYGKKKRSLVSMIIQIIVVI
                     LTAIAGYSMWKQPIFNIVFTKESVDFDGIKNFNETIGKMHQLNINLGNINDLQQGIDR
                     LILVFNGFFILCIVSLVITILTIVFNRTVLKVVNILVLTIMLVITLYFSYIIKALAQE
                     IADVFKKYYLNVPPDQVITEADAIHNGIILLGCSIGLLVISLFFRNRMPRLK"
     gene            8280..9200
                     /locus_tag="O2701_001239"
     CDS             8280..9200
                     /locus_tag="O2701_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCZ4293142.1"
                     /translation="MIRNRVMNSVVNKYLLHNRSIMFKNDQDVERFFYKREIENRKKH
                     KQPSTLNVKANLEKLSLDDMQVFRFNFRHQIDKKILYIHGGFNALQPSPFHWRLLDKI
                     TLSTLYEVVLPIYPKTPEFHIDDTFQAIQRVYDQLVSEVGHQNVVVMGDGSGGALALS
                     FVQSLLDNQQPLPNKLYLISPILDATLSNKDISDALIEQDAVLSQFGVNEIMKKWANG
                     LPLTDKRISPINGTIEGLPPVYMFGGGREMTHPDMKLFEQMMLQHHQYIEFYDYPKMV
                     HDFPIYPIRQSHKAIKQIAKSIDEDVTQNN"
     gene            complement(9308..9727)
                     /locus_tag="O2701_001240"
     CDS             complement(9308..9727)
                     /locus_tag="O2701_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293143.1"
                     /translation="MTDALQQKIHIELLDLLDDVKFELTELNAQKGLYINGPANQLLK
                     RGVHMAYVQGQKQAIDNIMTIVEQQLEDQHFLEHYDKFQNEVAHRNYDKTANFAELSD
                     IPRQFDNFLDQFYQIKGQYFIITHINTLIGDFHSEAH"
     gene            complement(9724..10215)
                     /locus_tag="O2701_001241"
     CDS             complement(9724..10215)
                     /locus_tag="O2701_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCZ4293144.1"
                     /translation="MVIIKQLFTHTQTVTSEFIDHNNHMHDANYNIIFSDVVNRFNYS
                     HGLSLKERENLAYTLFTLEEHTTYLSELSLGDVFTVTLYIYDYDYKRLHLFLTLTKED
                     GTLASTNEVMMMGINQHTRRSDAFPESFSTQIAHYYKNQSTITWPEQLGHKIAIPHKG
                     ALK"
     gene            10312..11259
                     /gene="corA"
                     /locus_tag="O2701_001242"
     CDS             10312..11259
                     /gene="corA"
                     /locus_tag="O2701_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCZ4293145.1"
                     /translation="MALKIRYQTTYEPFKVVDDIKEIPKDATIVWYDFDEPNEQENEW
                     FKAHFNFNDLEVDDAINGMPRAKYKSYKDYQYLVFHSIMGSNYSPIALNIFIQDNVLV
                     TYHHQTLESLNKVVYKYMNTLDAELDCADVVILILDMMVDKYFNFVYALEDSVYHFED
                     RHVDDRFNKMVMDSVFKLRSDLIKVKRVLFPMQELIDTMKQNGDLIIDNKHSLYIQHI
                     DDHLIKQRNIIRTAQEMTNEIRENFESYTSFRMNSIMQVLTLVSVIFSPLTFIAGIYG
                     MNFVNMPALHLHYGYYICLAVMFVIAVVLIIFFRRKKWF"
     gene            11290..12339
                     /locus_tag="O2701_001243"
     CDS             11290..12339
                     /locus_tag="O2701_001243"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCZ4293146.1"
                     /translation="MSDFQREQRKNEHVEIAMAQSDAMHSDFDKMRFVHHSIPSINVN
                     DIDLTSQTPDLTMAYPVYINAMTGGSEWTKNINEKLAVVARETGLAMAVGSTHAALRN
                     PRMAETFTIARKMNPEGMIFSNVGADVPVEKALEAVELLEAQALQIHVNSPQELVMPE
                     GNREFVTWLDNIASIVSRVSVPVIIKEVGFGMSKELMHDLQQIGVKYVDVSGKGGTNF
                     VDIENERRANKDMDYLSSWGQSTVESLLETTAYQSEISVFASGGLRTPLDAIKSLALG
                     AKATGMSRPFLNQVENNGIAHTVAYVESFIEHMKSIMTMLDAKNIDDLTQKQIVFSPE
                     ILSWIEQRNLNIHRG"
     gene            complement(12387..13595)
                     /gene="gltS"
                     /locus_tag="O2701_001244"
     CDS             complement(12387..13595)
                     /gene="gltS"
                     /locus_tag="O2701_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCZ4293147.1"
                     /translation="MIELNAITTLCLACILYLLGKAIVNHVNFLKRICIPAPVIGGLI
                     FAILVAALDSFGMVKIKLDASFIQDFFMLAFFTTIGLGASLKLFKLGGKVLLLYFMFC
                     AIISVIQNIVGVSLAKVLNIKPLLGLTAGSMSMEGGHGNAAAYGKTIQDLGIDSALTA
                     ALAAATLGLVFGGLIGGPVVKFLIKRYNLKPQHSDDTFKDYSQVAYNEHLHSKFNATE
                     VFFIQFTIVVFCMAVGSYFSHLFTAQTGINVPIYVGSLFVAVIVRNISESFNFNIVDL
                     KITNQIGDVALGIFLSLALMSIQLIEIYKLAIPLIIIVLVQVVVMILFAVLILFRGLG
                     KDYDAAVMVGGFIGHGLGATPNAMANLDVITKKYGNSPKAYLVVPIVGAFLIDLIGVI
                     VIMGFIQWFS"
     gene            complement(13932..14540)
                     /locus_tag="O2701_001245"
     CDS             complement(13932..14540)
                     /locus_tag="O2701_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HCZ4293148.1"
                     /translation="MDFVNNDTRQIAKNLLGVKVIYQDTTQTYTGYIVETEAYLGLND
                     RAAHGYGGKITPKVTSLYKRGGTIYAHVMHTHLLINFVTKSEGIPEGVLIRAIEPEEG
                     LSAMFRNRGKKGYEVTNGPGKWTKAFNIPRAIDGATLNDCRLSIDTKNRKYPKDIIAS
                     PRIGIPNKGDWTHKSLRYTVKGNPFVSRMRKSDCMFPEDTWK"
     gene            14939..15586
                     /locus_tag="O2701_001246"
     CDS             14939..15586
                     /locus_tag="O2701_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCZ4293149.1"
                     /translation="MEQLPQVGFMKSFVLFWKNYVNFKGRSRRSEYWYMALWHLILMV
                     PAVCVLIINLFLFFISIAADNETLIVMSILMIILTSVYTFVYSLATFIPNLALCVRRF
                     HDISRTMLFPMIMTVFSIIFYIVLQIIDSYYDSDFTLMPMGLNILLVLLYFVYLGLTI
                     VMIVFLCFDSKPANKYGESPKYPSTIKNQPVTSETPEKACTSETPKTPEETDNQL"
     gene            15817..16716
                     /locus_tag="O2701_001247"
     CDS             15817..16716
                     /locus_tag="O2701_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4293150.1"
                     /translation="MGLVIEHVTKRFGKMTAVNDISLKLESGKMLGFLGRNGAGKTTT
                     FRMILGLSEPTEGHITYNGKKLDKTMYNRIGYLPEERGLHGKLTVEEELKYLATLKGM
                     SKTEIQQQISYWLERFDITENRKKRIDSLSKGNQQKIQLLASMLHKPELLILDEPFSG
                     LDPVNVELLKEAVKDLNDWGSTIVYSSHRMEHVEELCDDVCILDKGQLVVSGDINHVR
                     ASNGNKKVVIESETTLPDLTNIRGIIHSENMKQGLQLTIENEDVAKDIYQVVAHQGYV
                     KRFQVVEPSLQDIFIEKVGGKDA"
     gene            16709..17941
                     /locus_tag="O2701_001248"
     CDS             16709..17941
                     /locus_tag="O2701_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4293151.1"
                     /translation="MHKFWATFTLTYIKKIKAKSFVIFMIIIAALMIGLSNIDKIINM
                     FDDGPDKIGVAAPNEQIYKVFKQQANTFHSDAKFTKVSIEDAEKEVKKHKLDKAYIIK
                     VNQNRTLQGTIISEKRVSHEDSQKVQALLTAIQTNMVAGELNINKEDLQKLQAQSKVD
                     NKVISNDEVDKVSEGQKIFNYALAYGIIFLMFFIVLNYASQIAMEIASEKTSRVIEMI
                     ITSISPIQHIFAKILGVIAVAVTQIILIVLMAVICIFAFDLKDLLQGFNVEMNQLSWQ
                     IIIVGIISSIVGITAYVLLAAILGSLTSRIEDLNQSLMPLTLLGMIAFYIAIFTINNP
                     DGQLALITSYIPFLAPFQLVVRAQTSGLQIYEVILSSLISIVMVAVLIWIAIKTYKDS
                     VLTFERGLFNSMKRVFKK"
     gene            17925..18110
                     /locus_tag="O2701_001249"
     CDS             17925..18110
                     /locus_tag="O2701_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293152.1"
                     /translation="MYLKNKIDESDTHLSYKMMMKACYSYFFIFSYRIMSKKVYNMNV
                     KTNFLKWECEVRRNVNK"
     gene            18159..18485
                     /locus_tag="O2701_001250"
     CDS             18159..18485
                     /locus_tag="O2701_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCZ4293153.1"
                     /translation="MLYPIFIFILAGLCEIGGGYLIWLWLREGQSSLVGLIGGAILML
                     YGVIATFQSFPSFGRVYAAYGGVFIIMSLIFAMVVDKQMPDKYDVIGAIICIVGVLVM
                     LLPSRA"
     gene            18515..19534
                     /locus_tag="O2701_001251"
     CDS             18515..19534
                     /locus_tag="O2701_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCZ4293154.1"
                     /translation="MIVEVEHQRQGVDLIKIDNDETKIIFTNYGARVVSWKYHDNNIV
                     LGNVVEADEFYHSNPFKFGASIGRYSGRIDNAKFKLKGKEYQLEKNNGEHHLHGGCHG
                     LDNKLFDYEIRNEIAQIKVIFKTVLKSADDHFPGDIDVTITHIYDADHQWSIEYEAVA
                     SEDTLFSPTNHVYFNLNRDNNVVDNHRISSNQLDMYVLDERNIVTGDILDLHEVFEDN
                     KIKLSDIFTSQHAQLSQQMTRFGGLDHPFTVGEHKMYVENHEFMLEVDTDMPHVVFFT
                     FNQPDEWDSPFNIYKPHSGFTLETQFLPNDINLYGEKAPSILKAGEVFNSKTSFRIEE
                     KVEND"
     gene            complement(19770..20423)
                     /locus_tag="O2701_001252"
     CDS             complement(19770..20423)
                     /locus_tag="O2701_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HCZ4293155.1"
                     /translation="MISIHAISTGKIQDLPYSSKRPMRSALDKTKISQTTWLSSTGFT
                     GDEQAYKDHGGPHKAVCGFSKHNYALYQDDLPTLPTHAMFGENLTFDYLDESDVYFGN
                     QYRLGEALIEVSEIREPCWKIQAKYNIPDLVKRMSTSGKTGFYFRVLKQGYVSPNDQL
                     YLIQEAPIEHRLSVQQLNDLYYNDRQNQDMLRYALNNPFLSPTRRDKLQKMYNRTLK"
     gene            20597..21283
                     /locus_tag="O2701_001253"
     CDS             20597..21283
                     /locus_tag="O2701_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HCZ4293156.1"
                     /translation="MKDVKALKLMTLNDVLSQINGDMTLGIGTGSTMELLLPQMAQLI
                     KERGYNITGVCTSNKIAFLAKELGIKICEINDVDHIDLAIDGADEVDPSLNIIKGGGG
                     ALFREKVIDEMASRFVVVVDETKIVQYLGETFKLPVEVDKFNWYHILRKIESYADIKV
                     ERRVNEDVAFITDNGNYILDCKLPKGIDPYKFHEYLIHLTGVFETGYFLDMADQVIVG
                     TQEGVKILEK"
     gene            complement(21349..22608)
                     /gene="sdpC"
                     /locus_tag="O2701_001254"
     CDS             complement(21349..22608)
                     /gene="sdpC"
                     /locus_tag="O2701_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpC"
                     /protein_id="HCZ4293157.1"
                     /translation="MKNNKISGFQWAMTIFVFFVITMALSIMLRDFQSIIGVKHFIFE
                     VTDLAPLIAAIICILVFKYKKVQLAGLKFSISLKVIERLLLALILPLIILIIGMYSFN
                     TFADSFILLQSTGLSVPITHILIGHILMAFVVEFGFRSYLQNIVETKMNTFFASIVVG
                     LMYSVFSANTTYGTEFAAYNFLYTFSFSMILGELIRATKGRTIYIATTFHASMTFGLI
                     FLFSEEIGDLFSIKVIAISTAIVAVGYIGLSLIIRGIAYLTTRRNLEELEPNNYLDHV
                     NDDEETNHTEAEKSSSNIKDAEKTGVATASTVGVAKNDTENTVADEPSIHEGTEKTEP
                     QHHIGNQTESNHDEDHDITSESVESAESVKQAPQSDDLTNDSNEDEIEQSLKEPATYK
                     EDRRSSVVIDAEKHIEKTEEQSSDKNK"
     gene            22898..23833
                     /locus_tag="O2701_001255"
     CDS             22898..23833
                     /locus_tag="O2701_001255"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HCZ4293158.1"
                     /translation="MYKQGEPNLWTGRLDSETDPKKFRHFQTVTFEDLSKLEKSSMPS
                     GVGILGYAVDKGVALNKGRIGAKEGPDAIKQAFAGLPDLNQCETLVDYGNVYHDHEEL
                     IDTQKEFAMLAAKSIANHRQTFLLGGGHDIAYAQYLATRKVYPTQSIGVINIDAHFDT
                     RAEQQSTSGTSFRQILEEDENTDYLVLGIAQGGNTQSLFDYAKEKKIDYVFADELLSH
                     VSPTIKDMIERFVHEHDVIMFTICMDVIDSAFAPGVSAPAVLGLYPHTVLELAKRIIP
                     SDKVSSVSIAEMNPTYDADNRTAKLVANLVHHFLK"
BASE COUNT         8433 a         3338 c         4316 g         7922 t
ORIGIN      
        1 ttaaagcaag gagaaagcag caacatgtaa atcttaattt gttatattat atatgggtca
       61 atatttttgt gttttttaga tgtgcgatac aatgattgct gaggatactt tataaaaata
      121 gtaattaggg agaaataatt atggtaaaac atttacaaga ccatattcaa tttttagagc
      181 agtttataaa taacgttaac gcattaactg caaaaatgtt gaaagattta caaaatgaat
      241 atgaaatttc attagagcag tctaacgtat taggtatgtt aaataaagaa cctttgacaa
      301 ttagtgaaat cacgcaaaga caaggtgtaa ataaggccgc agtaagccga cgaattaaaa
      361 agttaatcga tgctaaatta gttaagttag ataaaccaaa tttaaatatt gatcaacgtt
      421 tgaaattcat aaccttaact gacaaaggta gagcatattt gaaagaacgt aatgcgatta
      481 tgacagatat tgcgcaagat attactaatg atttaaattc tgaagatatt gaaaatgtaa
      541 ggcaagtatt agaagtcatc aatcatcgca ttaaaacata tagcaatcat aaataggttt
      601 ttgaaggtgt tcaaatataa gactaaatat gtatgtattg aacaccttct ttttagttat
      661 aattgagaga gtactttcat aaataatgta ataaggtaca attctaaagt aaagttaatc
      721 atttcttgaa ttgtattctt gggataatat ttgtaaaata ataatggtaa aataatgaaa
      781 taatttttgt gatatacata taacaattga agaggaacag aattgaggtg aaagtatgaa
      841 atcttgcccg aagtgcggac aacaagcaca agatgatgta cagatatgta cacaatgtgg
      901 acataaattt gatagtcgtc aagcattata tagaaagtca accgatgaag atatacaaac
      961 taataacatc aagatgagaa aaatggtgcc ttgggcaatt ggttttttca ttttaatctt
     1021 aattattata ttgttctttt tactaagaaa cttcaattca cctgaagcgc aaactaaaat
     1081 attagtgaat gcgattgaga ataatgataa acaaaaagta gcaacattat taagtactaa
     1141 agataataaa gtagattctg aagaagcaaa agtatacatt aactatatca aagatgaagt
     1201 tgggcttaag caatttgtca gcgaccttaa aaatacggta cataaattga ataagagtaa
     1261 gaccagcgta gcttcttata ttcaaaccag atctggtcaa aatatattac gtgtaagtaa
     1321 aaatggcaca cgttatatct ttttcgataa tatgagcttt actgcaccta ccaagcaacc
     1381 aattgttaaa ccgaaagaaa aaacaaaata tgagtttaaa tctggtggta agaaaaagat
     1441 ggttatagct gaagcaaata aagtgacgcc aataggtaat tttataccgg ggacatatag
     1501 aattccagct atgaaatcaa ctgagaacgg tgattttgca ggccatttaa aatttgattt
     1561 tagacaaagt aattctgaaa cggtagatgt tactgaagat tttgaagaag caaatatatc
     1621 tgtaacttta aaaggcgata caaaattaaa tgatagttct aaaaaagtaa ctataaatga
     1681 ccatgaaatg gcattttcaa gttccaaaac gtatggtcca tatccacaaa ataaagatat
     1741 taccatttca gcttcaggta aagcgaaaga taaaacattt acaacacaga cgaagacgat
     1801 taaagctagc gatttaaaat acaatacaga gataactttg aattttgaca gtgaagatat
     1861 cgaagactat gttgaaaaga aagaaaaaga agaaaacagc ttgaagaaca aattgataga
     1921 attctttgct ggatattctt tagcgaataa tgctgcgttt aatcagtcgg attttgattt
     1981 tgtatcatca tatataaaaa aaggatcatc tttttatgat gatgtaaaga agcgtgtatc
     2041 taaaggaagt ttaatgatga ttagttcacc acaaattata gatgctgaaa aacatggtga
     2101 taagattacc gcaaccgtaa gattaataaa tgaaaacggt aagcaagtag ataaagaata
     2161 tgagcttgaa caaggctcgc aagaccgctt gcaattaatc aagacatcag aaaaatagag
     2221 tataaaactt aaataagatg catgtgtcat ggaatacaaa caacatcctt tcaaagtatt
     2281 tacaattagt gtgaattact ttgaagggat tgttttttgt ggaaaaattt atgaagtgct
     2341 gaggtttata tcattttata ccttttataa gttgtaatga tgttcatgaa acttgtgact
     2401 aatgctaata tataaaagag catatttaaa tttagtttct gttcgtgata gttataaact
     2461 aatcattata taaaggagat agtcatatga atgatttaac gcattttaac aagcgttcac
     2521 cgatatttat tattatttta ggatctttga cggccattgg agcattatcg atagatatgt
     2581 ttttgcctgg attaccagat attagacatg attttcaaac aacgacgtct aacgcacaat
     2641 taacattatc gatgtttatg attggtttag cgtttggtaa tttgtttgct ggtccaattt
     2701 cagattcaac tgggcgcaga aagccgctta ttatagctat gattattttt acattagcaa
     2761 gtttaggtat tgtttttgta cataatattt ggctgatggt tgcattaaga tttcttcaag
     2821 gagtgacagg tggcgcagca gcagttattt caagagctat agcaagtgat atgtatagtg
     2881 gtaatgaact aactaagttt atggcattat tgatgcttgt taatggtatt gcaccagttg
     2941 ttgcgccaac aatagggggc ataattttaa attattctgt ctggcgtatg gtctttgtca
     3001 ttctgactat ctttggcttc gtcatggtca taggttcttt attaaaagta cctgaatcat
     3061 taacagtgac aaatcgtgag tcaagtagtg gattaaagac aatgtttaaa aacttcaaaa
     3121 tattattgaa gacgccacgt tttgtattac caatgctcat tcaaggtatg acatttgtaa
     3181 tattatttac ttatatttct gcgtctccgt ttattattca aaaaatttat ggcatgactg
     3241 caatacaatt tagttggatg tttgcaggaa ttggtattac gctcattata tcaagtcaac
     3301 ttactgggta ccttgttgat tttatagact cacaaaaatt gatgagaggc atgacgatga
     3361 ttcagattat cggtgtaatt ttagtgacga ttgtattgct taatcattgg aacttttgga
     3421 ttttagcgat aggatttatc attttaatag caccagtgac aggtgttgcg acacttggtt
     3481 ttacaatagc tatggatgaa agtagtagtg gtagagggag ttcatcaagt ttattaggtt
     3541 tagtacagtt tttatttggt ggtgttgcat cgccattagt gggggtaaaa ggtgaagata
     3601 atcctatacc ttacataatt attattatag ctacagcagt tatattaatt attttacaga
     3661 tttataatat gaaggtattt aaaacaaatc gatgataaac atagtttctt ctattatatt
     3721 gaattgaaaa attaaaaaac aaaacttgag ctattcatag acctatgaat ttagcatcaa
     3781 aaataaaaat gccgagatta ttatgtggaa atctcggcat ttttatattg atagcaccgt
     3841 tacatatgta ctattatcta tccatttggt ttaactttat ctagttgttt tatagcattt
     3901 ctttattaga atgaaaccgt attattagtt ttgaagttta atcgtattag ccaataaaat
     3961 gtcagcaact tcttcaggtg attcattaca accattcgaa agccaattaa taaacacccc
     4021 agcttgaccg cctatgatat atgtaacata aagctgttta ttggttactg tagtactata
     4081 gtcattcata atttgttgat agtaatctct cgctaaattt atatagtcta tgaataatgt
     4141 tttcattgga taggtaacta aaatgtcttt gaaaaactgc tcattgtgct tgatatactt
     4201 agcaataata atgaagaatt tataaagttt aagcttatcc tgtttaatat tttcgaaatt
     4261 atgatgtagc gcgcttagaa gaaattggaa tttttcctta tgctttgagt tgatggtatc
     4321 caataaatca aatttatctt tgtaataatc ataaaaagta gaacgattaa tattacaata
     4381 cgcgcaaatc attttagtgg atatttcttg aaaaggatat tcagccatca aatcaacaag
     4441 tgcgttttga atttctattt tggcttgtcg tttcatattg ttacaccttt gtgaatttta
     4501 accaacactc ttataaaaat tgttggtgtt tttttagtta tcatgtctct ataataaata
     4561 aagtgaattt taaaagcaaa taggaggaaa tgtttcatga aaaagatgat attaattaat
     4621 gtcattacgg ttgttgtact gttagcaatt gggattgctg gcttttattt ttggaataaa
     4681 acaacaagct atgtaacaac tgataatgca aaagtaaatg gcgatcaaat taaaatagca
     4741 agtcctgcat ctggacaaat taaatcactt aatgttaaac aaggtgacaa actcgataaa
     4801 ggtgacaaag tagcaattgt tactgtacaa ggtcaagatg gcgaaacgaa agatatggat
     4861 cttaaaatgc ctcaaaaagg tacaattgcc aaattagatg gtatggaagg ttcaatggtg
     4921 caagctggta acccaatcgc ttatgcatac aatttagatg atttatatgt aacagcaaat
     4981 attgacgaaa aagatattaa agatgttgaa gtaggtaaag atgttgatgt gactattgac
     5041 ggtcaaaaag cttctattaa aggtaaagtt gatagtattg gaaaagcaac tgctgcaagc
     5101 ttttcattaa tgccatcatc aaatagtgat ggtaactaca ctaaagtttc tcaagtaata
     5161 cctgttaaaa tcactctaga atcagaaccg tctaaacaag ttgttcctgg aatgaacgct
     5221 gaagtaaaaa tccataaaaa ttaagggggt cattaaatga ctacgacctt cattattagc
     5281 tacattattt tagcgctcat tattgttggg gttatcaatt tatttttaat aagatcaaga
     5341 aaaaaaggca aacgccaaca aaaggaacaa caatttacga cacgtcaatc aaatcaatct
     5401 aaatttaaag ctagtgattt agacaaaaca actgatcaat cgacacaacg tatgacgcat
     5461 gaagagttgc gtgttgacaa tcaagatgat catagccaag ttagtctaaa tggttacaca
     5521 aaggggtctg agaaagatca agaagcattc actaataata aggatgagga agcagttgct
     5581 gctaaaaatc ctgaatcaga agaatataaa gtgaatgaaa aaataaaaaa agaacataaa
     5641 aactttattt ttggtgaagg tgtttcacgc ggtaaaatat tagcggcatt attattcggt
     5701 atgtttattg cgattttaaa ccaaacatta ttaaatgttg ctttaccaaa aattaatacg
     5761 gaatttaata tttctgcgtc aacagggcaa tggttgatga cgggattcat gttagtaaac
     5821 ggtattttaa taccaattac ggcgtatcta tttaataaat attcatatcg aaaattattt
     5881 ttagttgcgt tagtactctt tacaattggt tcattaatct gtgcgatttc aatgaatttc
     5941 ccaatcatga tggttggacg tgtattacaa gccattggtg caggtgtact aatgccatta
     6001 ggttcaattg ttattattac aatttatcca cctgaaaaac gtggtgctgc catgggtaca
     6061 atgggtatcg caatgatttt agcacctgca attggtccaa cattatcagg ttatattgta
     6121 caaaattacc attggaatgt gatgttttac ggtatgttca ttatcgggat tatcgctatt
     6181 ttaattggtt ttgtatggtt taagttatat caatatacaa caaaccctaa agctgatatc
     6241 ccaggtatca tttttagtac gattggtttt ggtgctttgt tatatggttt ctcagaagct
     6301 ggcaacaaag gttggggttc agtagagata gaaacaatgt ttgcgattgg tattatcttt
     6361 attattctat tcgttattag agaattaaga atgaaatcac caatgttgaa tttagaagta
     6421 ttgaaattcc caacatttac attaacaaca attattaata tggttgtaat gttaagttta
     6481 tatggtggta tgattttatt accgatttat ttacaaaatt tacgcggatt ctcagcatta
     6541 gattccggat tgttattatt acctggttct ctaattatgg gtctactagg accatttgca
     6601 ggtaaattgt tagatactat tggtttaaaa ccattagcta tctttggtat tgctgtaatg
     6661 acttatgcaa catgggaatt aactaaatta aatatggata caccatatat gacaatcatg
     6721 ggtatctatg tacttcgttc atttggtatg gcatttataa tgatgccaat ggtaactgca
     6781 gctattaatg cgttaccggg acgacttgcc tctcatggta atgctttctt aaatacgatg
     6841 cgtcaattag caggctctat aggtacagca atcttagtta ctgtaatgac aacacaaact
     6901 acacaacact tatcagcttt tggggaagag ttagataaaa cgaatcctgt tgtacaagat
     6961 catatgcatg aattagcatc acaatatggc ggacaagaag gcgcaatgaa agtgttacta
     7021 caatttgtaa ataaactagc aacggttgaa ggtattaatg atgcatttat agttgcaacg
     7081 atatttagca tcatcgcctt aattttatgt ttatttttac aaagtaataa aaaagcaaaa
     7141 gctacagctc aaaagttaga tgcagataat agtatcaatc atgaataaat aaaataaatt
     7201 aattgaagtg tgactaatca aaaattatgt tgtggggaca tgatttttaa agtatcggtg
     7261 ccaaatatgg ttatcgatac tttttttatt tgttgattta tagaatgtta gaggaattat
     7321 attaaaattt ggcattgacg tagtaggtca ttaataaaga aaaagcagga agtgggtcaa
     7381 cgaaatgaat tttgtgaaaa taacatttct gtcccaatcc ctactatata acattatttt
     7441 aaacgaggca tgcgattacg gaagaataag cttataacaa gtaaaccgat gctacagcca
     7501 agtaaaatga tgccgttatg aatagcgtca gcttctgtaa tcacttgatc tggtggtaca
     7561 tttaaataat attttttgaa aacatctgca atttcttgtg cgagtgcttt aataatataa
     7621 ctgaaataca atgtaatgac taacataatt gttaacacta aaatatttac tacttttaga
     7681 actgtgcgat taaatacaat tgtcagtatt gttatgacta ggctaacgat acataaaata
     7741 aagaaaccgt taaagactaa aattaagcga tcgattcctt gttgtaaatc attgatatta
     7801 cccaaattaa tatttaattg atgcattttt ccaattgtct cgttaaaatt tttaatgcca
     7861 tcaaaatcta cagattcttt ggtgaagaca atattaaaaa tgggttgttt ccacatgctg
     7921 tatccagcga ttgcggttaa tataacaaca atgatttgaa taatcatgct taccaatgaa
     7981 cgttttttct taccatatcg cttaccacga tgaatattcg gttgttgaaa tgtctcatca
     8041 aatggttcta atcgttgatt tggatttgtc atgcaaggta cctccgtata aagttctttt
     8101 ctttacttat caatatgtct agttgtctgt tataatgatg aaaaatcact gttcaattgt
     8161 agtcttaaat ttgaaagtga aaaagaaaat atcttctaca ttatatactt tttttggcaa
     8221 agaatgtagg ggatagacaa tcattggata cataaaatat ataaaggtag gttaagacat
     8281 tgattagaaa ccgtgttatg aatagcgttg tcaataaata tttgcttcac aatcgctcga
     8341 ttatgtttaa aaatgatcaa gatgttgaaa gattttttta taaaagagaa attgaaaata
     8401 gaaaaaaaca taagcagcct tcaacattaa atgttaaagc aaatttagaa aaattgtcat
     8461 tagatgatat gcaagtcttt cgctttaatt tcagacatca aattgataag aaaattttat
     8521 atattcacgg tggattcaat gcactacaac catcaccgtt ccattggaga ttgttggata
     8581 aaatcacttt aagtacatta tatgaggttg tactgcctat ctatccaaag acaccagagt
     8641 ttcatattga cgatactttc caagcgatac aacgtgttta tgatcaatta gtatctgaag
     8701 taggacatca aaatgtcgta gtcatgggtg atggttcagg tggtgcactg gcattatcct
     8761 ttgtacaatc tcttttagat aatcaacagc cattaccgaa taaattgtat ttaatctcac
     8821 caattttaga tgcaacacta tctaataaag atatttcgga cgccttaatc gaacaagatg
     8881 cggttctaag tcagtttggt gtcaatgaga ttatgaaaaa atgggcgaat ggcctaccat
     8941 taacagataa gcgcatatcg ccaattaatg gcacaataga aggattgcca ccagtttata
     9001 tgtttggtgg tggacgtgaa atgacacatc ctgatatgaa gctatttgaa caaatgatgt
     9061 tgcaacatca tcaatacatt gagttttatg attatcctaa gatggtacat gattttccaa
     9121 tttatccaat tagacaatca cataaagcta ttaaacaaat tgccaaatcg atagatgagg
     9181 atgtaacaca aaataactaa taacatataa atgacccagt tacgatttca tgcaagatga
     9241 tatttgacat gaaaacgtaa ttgggttttg tttttgaaaa tatagcaagg gttatataga
     9301 ctaattgtta atgagcttct gagtgaaaat caccaataag tgtattgata tgtgtgatga
     9361 taaagtattg ccctttaatt tgataaaatt gatctaaaaa attatcgaat tgacgtggaa
     9421 tatcactaag ttctgcaaag ttagctgttt tatcataatt gcgatgagca acctcatttt
     9481 gaaatttatc ataatgttct aggaaatgtt gatcttcaag ctgttgttcc acaatagtca
     9541 taatattatc gatggcttgc ttttgtcctt gaacataagc catatgcacg ccacgcttaa
     9601 gtagctgatt tgctggtccg ttaatgtata accctttttg tgcatttaat tctgttaatt
     9661 caaacttaac atcatctaat aaatctagta attcgatatg aatcttttgt tgtaatgcat
     9721 ctgtcatttt aatgctcctt tgtgtggaat tgctatttta tgtcctaatt gttcaggcca
     9781 agtgatagtt gattgatttt tataatagtg tgctatttgt gttgaaaatg attcaggaaa
     9841 agcatcagaa cgacgtgtgt gctgattaat tcccatcatc attacttcat ttgttgatgc
     9901 tagtgtacca tcttctttag ttaatgttaa aaataaatgc aaccgcttat aatcgtaatc
     9961 ataaatatat aaagtaacag taaatacatc gccaagagac aattctgaga ggtatgtcgt
    10021 atgttcttct agtgtaaata gcgtatatgc taaattttcg cgttctttta aagaaagacc
    10081 gtggctgtaa ttaaaacgat tcacgacgtc actaaaaatg atattataat ttgcatcatg
    10141 catatgattg ttatggtcaa tgaattcaga tgttacggtt tgagtatgtg taaaaagttg
    10201 tttgataata accatctcct tagtgactat catactaaaa taaattaaga catacacgtt
    10261 atttaattca tgataaaata atagagaatt cgttatttag ggggagtatc gatggcttta
    10321 aagatacggt atcaaactac atatgaaccg tttaaagtag ttgatgatat caaagagata
    10381 ccaaaagatg cgactattgt ttggtatgat tttgatgaac cgaatgaaca agaaaatgaa
    10441 tggtttaaag cacattttaa ttttaatgat ttagaagtgg atgatgcaat taacggtatg
    10501 ccacgagcta aatataaatc ttataaagat tatcaatact tagtattcca tagtattatg
    10561 gggtcaaact attcaccaat agcactaaat atttttatac aagacaatgt attagtaaca
    10621 tatcatcatc aaacgttgga atcattaaat aaagtagtct ataagtatat gaatacatta
    10681 gatgcagaat tagattgtgc agatgtcgtc atattaattt tagatatgat ggttgataaa
    10741 tatttcaact ttgtatatgc acttgaagat agtgtttatc attttgaaga ccgccatgta
    10801 gatgatcgct ttaataaaat ggtcatggat agtgtcttta agttacgttc ggatttgatt
    10861 aaagtaaaac gtgtactgtt tcctatgcaa gagctaattg atacaatgaa acaaaatggt
    10921 gatttgatta tagataataa acattcgcta tacattcaac atatcgatga ccacttgatt
    10981 aagcaacgta atatcattcg tactgctcaa gaaatgacaa acgaaatacg agaaaatttt
    11041 gagtcataca cgtcatttag aatgaatagt attatgcaag tactgacatt agtatcagtt
    11101 atattttcac cgttaacatt catagctggg atttatggta tgaactttgt aaatatgccg
    11161 gcattacatt tacattatgg atattatatt tgcttagctg taatgtttgt tatagctgtc
    11221 gtattaatca tctttttcag acgtaaaaaa tggttttaaa ttagaaatga cgaaaaggta
    11281 ggtttaatca tgagtgattt tcaaagagaa cagagaaaaa atgaacatgt tgaaatagca
    11341 atggcccaat ctgacgcaat gcattcagat tttgataaga tgcgttttgt gcatcattct
    11401 atcccatcaa ttaatgtaaa tgatatcgat ttgacatcac agacgcctga cttaacgatg
    11461 gcatatccgg tttatattaa tgcaatgacg ggtggtagcg agtggacgaa aaacatcaat
    11521 gaaaagctag ctgtagttgc aagagaaact ggcttagcga tggcagttgg atcaacacat
    11581 gcggcattga gaaatccacg catggctgag acgtttacga ttgcgcgaaa aatgaatcct
    11641 gaaggcatga tttttagcaa tgttggtgcg gacgtaccag tagaaaaggc tttggaagca
    11701 gttgaattac ttgaggcaca agcgttacaa atccatgtta attctcctca agaattagtt
    11761 atgcctgaag ggaatcgtga atttgtgact tggttagata atatagcgtc gattgtatca
    11821 cgagtgtctg ttccagtcat tataaaagaa gttggatttg gtatgagcaa agaattaatg
    11881 catgacttac aacaaatagg cgtcaagtat gtcgatgtta gtggcaaagg tggtactaac
    11941 tttgtagata ttgaaaatga acgtcgtgca aataaagata tggattactt atcatcatgg
    12001 ggacagtcta cagttgagtc attacttgaa acaacggctt atcaaagcga aatttcagtt
    12061 ttcgcgagtg gtggtttacg tacaccactc gatgcaatta aaagtttagc acttggtgca
    12121 aaggcaactg gtatgtcacg tccgttttta aatcaagttg aaaataatgg cattgcacat
    12181 acagtagctt atgtagaatc atttattgaa cacatgaaat caataatgac gatgttagat
    12241 gctaaaaata ttgacgactt aacacaaaaa caaatcgtat ttagtccgga aatattgtca
    12301 tggatagaac aacgtaactt gaatatacat cgaggataaa taaaagagga ggcgaaggcc
    12361 tcctctttta tttatgagtt tggtgtttaa ctaaaccatt gtatgaatcc cataatgact
    12421 ataacaccaa ttaaatcgat taagaatgca ccaacaatag gtacaactaa atatgcttta
    12481 ggtgagtttc catatttttt agtaataaca tctaaatttg ccatggcatt tggcgttgca
    12541 ccaagcccat gaccgataaa accacctacc attactgcag catcataatc ttttcctaaa
    12601 cctctaaata aaattaaaac agcaaataaa atcataacga caacttgaac taaaacgata
    12661 ataataagag gtatagcaag tttataaatt tcgattaatt gaatgctcat tagcgcaaga
    12721 gataagaaaa tacctaatgc gacatcgcca atttgattag taatttttaa atctacaata
    12781 ttaaaattaa aactttcaga gatatttcgg acaataacag ctacaaataa tgagccaacg
    12841 taaattggaa cattaatccc tgtttgagct gtaaacaaat gactgaaata acttccaact
    12901 gccatacaga atacaacgat tgtaaattga atgaagaata cttcagtggc attaaattta
    12961 ctatgtaaat gttcgttata tgctacttgg ctataatctt taaatgtatc gtcactatgt
    13021 tgaggcttta agttataacg tttgattaaa aattttacaa ctggaccacc gataagccct
    13081 ccaaatacaa gacctaaagt tgcagctgca agagccgctg tcagtgccga atcaatacct
    13141 aaatcttgaa ttgtcttacc ataagcagca gcattaccat gaccgccttc catagacatg
    13201 gaacctgctg ttaatcctaa caaaggttta atatttaata cttttgctag tgatacacca
    13261 actatgtttt gaatgactga aatgatagca caaaacataa agtataatag caagactttg
    13321 ccacctaatt taaataattt caatgatgca ccaagaccga ttgtcgtaaa gaatgctaac
    13381 atgaagaaat cttgaatgaa tgaagcatct aatttaatct taaccatgcc aaatgaatcc
    13441 aaagccgcaa ctaaaatagc aaagattaag ccgccaatca ctggtgctgg tatacaaata
    13501 cgttttaaaa aattaacgtg attaacgata gccttaccaa gtaaataaag gatacaagct
    13561 aaacataatg ttgtaattgc attaagttct atcattttca aatccccctt gttgaatatc
    13621 cctctcccta aataagcttt tatatgtaag cgttataaaa gggcgctttt aacattatac
    13681 atgcaacaaa tcaatatttc tagtttttga aaataatatc aataatatta atgcatctaa
    13741 atttaataat tataaataat tctattgtta tattttataa ttaagaacaa gttcaataag
    13801 aggagatgaa tcaaaaaata tgatggatac aaagaaaaac aatcaaccca tccgcatgtt
    13861 gacggcacga ttgattgact tataaggaga tagatttcat tttcatgata gtaatcaatg
    13921 aaagatggca tttatttcca agtatcttcg ggaaacatac aatctgattt acgcatgcga
    13981 gacacaaatg gattaccttt cactgtgtaa cgtaaagatt tatgtgtcca atcaccttta
    14041 tttggaatac cgattcgtgg actagcaata atatctttag gatatttacg attcttagta
    14101 tcaatagaca atctacagtc atttaacgta gcgccatcga tagcccgtgg aatgttaaat
    14161 gccttagtcc attttcctgg gccattcgtt acctcgtagc ctttcttacc tctgttacgg
    14221 aacatagcgg ataaaccttc ttctggttca attgcgcgga taagtacgcc ttcaggtata
    14281 ccttcagatt ttgttacaaa attaatgagt aaatgcgtat gcatgacatg tgcataaatt
    14341 gtaccaccac gtttatataa tgacgtgact ttaggtgtta ttttaccgcc atagccatga
    14401 gccgcacgat cattcaaacc taagtaagct tccgtttcca cgatgtagcc tgtatacgtt
    14461 tgagtggtat cctgataaat cactttgaca cctaataagt ttttagcaat ttgtcttgta
    14521 tcattattaa cgaaatccat aatattctcc tcgcaattat atgattaatt taaataaaaa
    14581 tgaaaaagat atttttattg tggtaaatgt ttgataaaat agtattgtta agaattataa
    14641 aaatgtaaag tatatacttt gttatggaat attttatttg catagattga gactctttta
    14701 aacgtgaaca ttgcagtatg acatattttt agtatagtta acgaagcgtt gactttcaaa
    14761 tgagtctcca tatagatgtg tacttgtagc gggttttcat tttactagtg atgaaatata
    14821 aagttcattt tccaaagtga ttgacgattg aaatgaagtg gcttgataat gaatcttgta
    14881 aattataacg gtaacaataa ttaaaaatat aaatatgatt aacgaaaggg agattacaat
    14941 ggaacaatta cctcaagtag gttttatgaa atcattcgtt ttattttgga aaaattatgt
    15001 aaattttaag gggagatcaa gacgtagtga atattggtat atggcactat ggcatttgat
    15061 acttatggtt ccagctgtgt gtgtgctaat aataaaccta tttctattct ttatttctat
    15121 agcggcggat aacgaaacat taatagttat gagtatattg atgattatcc ttacatctgt
    15181 gtatactttt gtctatagtt tggcaacgtt tattccaaat ttagcgttgt gcgttcgaag
    15241 atttcacgat atatcaagaa caatgttatt tccaatgatt atgacagtat tcagtattat
    15301 attttatata gtattacaga ttatcgatag ttattatgac agtgatttta cattaatgcc
    15361 gatgggtctt aatattttgt tagtacttct ttattttgtt tatttaggcc taacaattgt
    15421 tatgatagta ttcctatgct ttgacagtaa gccggcaaat aaatatggcg aaagcccaaa
    15481 atacccgtct acaattaaaa atcaaccagt gacatctgaa acaccggaaa aagcgtgtac
    15541 atctgaaaca ccgaaaacac cggaagaaac agataatcaa ttgtaatgat ataaatatct
    15601 cggcacaatg atgctgggat atttttttac aaataaggca ataaaggcat ggaaaaaaca
    15661 tatctagtgc taatgatact taacatgcaa aaatggcact atttaaattt ttcaatttca
    15721 gaaattttaa aaataaattc attttaaaaa tattgcattt atagagaatg gaaatgttat
    15781 attaagataa atacatattt ttttaggagg caaagtatgg gacttgtaat tgaacatgtt
    15841 acgaaacgtt ttggcaagat gacggcagta aatgatattt cattaaagtt agaatcaggt
    15901 aaaatgttag gttttctagg aagaaatggt gcaggtaaaa caacgacatt tcgtatgatt
    15961 ttaggcttaa gtgaaccaac tgaaggacac attacgtata acggtaaaaa actagataaa
    16021 acaatgtaca atcgtattgg ttatttaccg gaagaacgcg gtttacatgg gaaattgaca
    16081 gttgaagaag aattgaaata tttggcaact ttaaagggaa tgtcaaaaac agaaatacaa
    16141 cagcaaatat cgtattggct tgagcgtttt gatattactg aaaaccgcaa aaaacgaatt
    16201 gatagtctat caaaagggaa tcaacaaaaa attcagctgt tagcaagtat gttacataaa
    16261 ccagaattac taatattaga tgagcctttc agtggtctag acccagttaa tgttgagtta
    16321 ttaaaagaag cagttaaaga cttaaatgat tggggcagta caatcgttta tagttctcac
    16381 cgtatggagc atgttgaaga attatgtgat gatgtttgta ttttagataa aggtcaactt
    16441 gttgtttctg gtgatatcaa tcatgtacgt gcatctaatg gtaacaagaa agttgtcatt
    16501 gaatcagaaa caacactacc agatttaaca aacattagag gcatcattca tagtgaaaac
    16561 atgaagcaag gtcttcagtt gacgattgag aatgaagacg tagccaaaga tatttatcaa
    16621 gttgtggcac atcaaggtta tgttaaacga ttccaagttg tagaaccatc attacaagat
    16681 atattcattg agaaggttgg tggcaaggat gcataagttt tgggcgacat ttactttgac
    16741 atatatcaaa aaaattaaag ctaaatcatt tgtaattttt atgattatta ttgctgcctt
    16801 aatgattggc ctatctaaca ttgataagat tattaacatg tttgatgatg gtccagataa
    16861 aattggtgta gcagcaccga atgaacaaat ctataaggtt ttcaagcaac aggcgaatac
    16921 atttcatagt gatgctaagt ttactaaagt ttctatagaa gatgctgaaa aagaagtaaa
    16981 aaaacataaa cttgataaag cttatattat caaggtgaat caaaatcgca cgttacaagg
    17041 gacaatcatt agtgagaagc gagtaagtca tgaagattca caaaaagtac aagcactgct
    17101 tacagctatt caaacaaata tggtagccgg ggaattaaat atcaacaaag aagacttaca
    17161 aaaattacaa gcacaaagta aagttgataa taaggtgatt tctaatgatg aagtcgataa
    17221 agtatcagaa ggacaaaaga ttttcaacta tgctttagct tatggcatta tctttctaat
    17281 gttcttcatt gttttaaatt atgcatctca aatagctatg gaaattgcta gtgagaaaac
    17341 atcgcgtgtc attgaaatga ttattacaag tatttcacca atccaacata tttttgctaa
    17401 aattttaggc gttatagcag ttgccgtgac tcagattatt ttaattgtat tgatggcagt
    17461 tatatgcatt tttgcctttg acttaaaaga cttgctacaa ggttttaacg tggaaatgaa
    17521 tcaattgtct tggcaaatta ttattgtcgg aattataagt agtattgttg gaattacggc
    17581 gtatgtatta cttgcagcca tcctcggatc attaacatct agaatagaag atttaaatca
    17641 atctttgatg ccactgacat tacttggtat gattgcattt tatatcgcga tattcacgat
    17701 aaataatcct gatggacaac ttgctttaat cacaagttat attccgtttt tagcgccatt
    17761 tcaattagtt gtacgcgctc aaacttcagg gcttcaaata tatgaggtca tcttaagttc
    17821 tttaatctca attgtaatgg tagctgtctt gatatggatt gcaattaaaa catataaaga
    17881 ttcagtactt acatttgaac gtggcttatt taattcaatg aaacgtgtat ttaaaaaata
    17941 aaattgatga gagtgacaca catttatcat ataaaatgat gatgaaagcg tgttactctt
    18001 atttttttat atttagctat agaataatgt ctaaaaaagt gtacaatatg aatgtgaaaa
    18061 cgaacttttt aaaatgggaa tgtgaagtaa gaagaaatgt aaacaaatga tatgtagccg
    18121 atagattatt gatagcaaat gtgtgatgga gtgatttcat gttatatccg atttttatat
    18181 ttatattagc tggcttatgt gaaataggtg ggggatacct gatttggctg tggcttaggg
    18241 aaggacagtc ttcacttgtt gggctaatag gcggtgcgat actcatgtta tatggtgtca
    18301 ttgcgacatt tcaatcattt ccatcattcg gaagagtata tgcagcatat ggtggcgtat
    18361 ttatcatcat gagtttgatt tttgcgatgg tcgtagataa acaaatgcca gataaatatg
    18421 atgttatcgg tgcaatcatt tgtattgtag gtgttttagt aatgttgcta cccagcagag
    18481 cttgaacact tgtggttaga agggagaagt aaaaatgata gttgaagttg agcaccaaag
    18541 acaaggtgta gatttaatca aaattgataa tgatgaaact aaaattattt ttacgaatta
    18601 tggagcaaga gtagtgtctt ggaaatatca tgataataat attgttcttg gaaatgttgt
    18661 tgaagcggat gaattttatc atagtaatcc ttttaaattt ggtgcttcta taggaagata
    18721 tagcggtaga attgataatg ctaaatttaa gttgaaaggt aaagaatatc agttagaaaa
    18781 aaacaacggt gaacatcatt tacatggtgg atgtcatggt ttggacaata agttatttga
    18841 ttatgaaatt agaaatgaaa ttgctcaaat taaagttata tttaagacgg tgttgaaatc
    18901 agctgatgat catttcccgg gtgatatcga tgtgacgatt acacatattt atgatgcgga
    18961 tcaccaatgg tcgattgaat acgaagcagt tgcatcagaa gacacattgt ttagtcctac
    19021 gaatcatgtg tacttcaatt tgaatcgaga taataatgtc gttgataatc atcgcatttc
    19081 aagtaatcaa ttagatatgt atgtattaga tgagcgaaat attgttacag gtgatattct
    19141 agatttacat gaagtgtttg aagataacaa gataaaattg agtgatattt ttacatcaca
    19201 acatgcgcaa ttaagccaac aaatgacacg ttttggtggt ctcgatcacc catttacagt
    19261 tggtgaacat aaaatgtatg ttgaaaatca tgaatttatg ttagaggtcg atacagatat
    19321 gcctcatgtt gtgttcttta cttttaacca gcctgatgag tgggatagtc catttaatat
    19381 ttataaacca cattctggct ttacattaga aacacaattt ttaccaaatg atattaattt
    19441 atatggtgaa aaagcaccgt caattttaaa agctggtgaa gtgttcaatt cgaaaacaag
    19501 tttccgtatc gaagaaaaag tagaaaatga ttaaaagtat cggcatttat ataatattta
    19561 tgttatttgg cggaattttg aataggttat caagaatcgt atcaaatgaa gtgacatcat
    19621 taatatacag tttgaaataa agaaaatata taacataaaa aaggaatcaa tagcatcagt
    19681 ggttatagat aagactatta tacaaacgat gtgtaagttg attccttttt gttttgaaaa
    19741 gttcatttaa cacttataat gaaaggtaat tatttcaatg ttctgttata cattttttga
    19801 agtttatcgc gtcttgttgg tgacagaaat ggattgttta gtgcatatcg taacatatct
    19861 tgattttgtc tatcattata ataaaggtca ttaagctgtt gtacagataa acgatgttcg
    19921 attggtgctt cttgtattaa gtaaagctga tcatttggag atacatagcc ttgttttaat
    19981 acccggaaat agaaacctgt tttaccagat gtagacatgc gcttcactaa atcaggaata
    20041 ttatattttg cttgaatttt ccagcatggt tctctaattt cagaaacctc aattaacgct
    20101 tcacctaaac gatattgatt accaaagtaa acatcagatt cgtctaaata atcaaatgtt
    20161 aaattctctc caaacatcgc atgagtaggt agtgtaggta aatcatcttg atacagtgca
    20221 taattatgct tactaaaccc acaaactgct ttatgtggtc caccatgatc tttataagcc
    20281 tgttcatcac cagtgaaacc agttgaagat aaccatgttg tttgtgaaat cttagtttta
    20341 tctaaagcag agcgcatcgg tcttttcgag ctataaggca aatcttggat ttttcctgtc
    20401 gaaattgcgt gaattgatat catatcattc tccttaacga gtattattta aaatatattg
    20461 tactataaaa catttttaga attgtaaatt ctgattgata tgtttaaaaa aatgttagaa
    20521 ataagggtat gttaatttat acgagtcatg taaaatatga attgaataga ttacataaat
    20581 atgggaggct accttcatga aagatgtcaa agcactcaag ttaatgacat taaatgatgt
    20641 tttaagtcaa atcaatggtg acatgacgct gggaattggt acaggaagta caatggaatt
    20701 actcttacct caaatggcgc aactaattaa agaacgtggt tacaatatta caggtgtatg
    20761 cacttctaat aaaattgcat ttttagcaaa agaattaggt attaaaatat gtgaaatcaa
    20821 tgatgttgat catatcgact tagcaattga tggtgctgat gaagtagatc catctttaaa
    20881 tataattaaa ggtggcggtg gtgcgctgtt cagagagaaa gttatagatg aaatggcatc
    20941 gcgttttgtt gtggttgtcg atgaaacgaa aatagttcaa tacttaggtg agacgtttaa
    21001 gttaccagta gaggtggata aatttaactg gtatcacata ttacgtaaaa ttgaatcata
    21061 cgctgatata aaagtagaac gtcgtgtaaa cgaagatgtc gcatttataa ctgataatgg
    21121 caattatatt ttagattgta agttaccaaa aggaattgac ccatataagt tccatgagta
    21181 tttaattcat ctgacaggtg tgtttgagac aggatacttc ttagatatgg cagatcaagt
    21241 tattgtaggc actcaagagg gtgttaaaat tttagaaaaa taatatatag tatagaaaag
    21301 cgcttgctag catcatcttt ttaagaggta ctagcaagcg ctttgttatt atttgttttt
    21361 atctgaagat tgttcttcag ttttttcgat atgtttttct gcatcaatta caactgatga
    21421 acgtctgtct tctttataag tcgcaggttc ttttaatgat tgctctattt catcttcatt
    21481 tgaatcgttt gttaaatcgt cactttgtgg tgcttgttta actgattctg ctgattctac
    21541 tgactccgaa gtgatgtcat gatcttcatc atgattagat tcagtttgat tacctatgtg
    21601 atgttgaggt tctgtttttt cagtaccttc atgaatgctt ggttcgtcag ccactgtatt
    21661 ttcagtatca tttttagcaa caccaaccgt tgatgcagta gctacacctg ttttttcagc
    21721 atctttaata tttgaagaag atttttcagc ctcagtatga ttagtttctt catcgtcatt
    21781 gacatggtct aaataattat taggctcaag ttcttcaagg tttcgtcttg ttgttaaata
    21841 tgcaatacct cggataatta agcttaaacc aatgtatcct actgcaacga ttgctgttga
    21901 aatggcgatg actttgattg aaaatagatc gccgatttct tcgctaaaca agaaaataag
    21961 tccgaatgtc attgaagcat ggaatgtcgt tgcaatataa attgtacgtc ctttagtcgc
    22021 tctaattaat tcaccaagaa tcatagagaa tgagaatgta taaaggaagt tataagcagc
    22081 aaattctgta ccataagttg tatttgctga gaaaactgaa tacatcaaac caacaacaat
    22141 acttgcaaag aacgtgttca ttttcgtttc aacaatattt tgtaagtatg aacggaatcc
    22201 gaattctact acgaacgcca tcagaatatg tccaatcaga atgtgtgtaa taggtactga
    22261 taagcctgtt gattgtaata aaataaagct atctgcaaat gtattaaagc tgtacatacc
    22321 aataattaga ataattaaag gtaaaattaa agctagcaat agacgttcaa ttactttcag
    22381 gctgattgag aattttaaac ctgcaagttg gaccttttta tatttgaaaa cgagtataca
    22441 aatgattgca gcaattaatg gtgctagatc tgtaacttca aatataaagt gtttgacacc
    22501 aattatagac tggaaatctc tgagcataat ggataacgcc attgtaatga caaagaagac
    22561 gaaaatcgtc attgcccatt gaaaaccaga aattttattg ttcttcatta tatgtaacct
    22621 ccattaggta acaagcaaaa tatcttttag taacaattat acatattaca agccgagaga
    22681 taaattgtct atgtcaatat taatgaaaat gtaatagtct tgacgtattt tattaacata
    22741 aatcagctaa gtattcgtta ttacatagaa aataggggta aaagtaatgt agataagtat
    22801 ttttaagata cggatttaac tatgaattga attcaaaatt tcctatacgt tatactttaa
    22861 ttgttaataa gccacataaa taagaggggg aaatgctgtg tacaagcaag gtgaaccaaa
    22921 tttatggact ggaaggttag atagtgaaac agacccgaaa aaatttagac attttcaaac
    22981 agtaacattt gaagatttgt ctaagctgga aaagagtagt atgccatcag gggtcggtat
    23041 attaggctat gctgttgaca aaggtgttgc tttaaacaag gggcgcattg gtgcaaaaga
    23101 aggaccagat gcgattaaac aagcatttgc aggtttgccg gatttgaatc aatgtgaaac
    23161 tttagtcgat tacggaaatg tttatcatga tcatgaggaa ttaattgata ctcaaaaaga
    23221 atttgctatg cttgcagcga agtcaattgc taatcataga caaacatttt tattaggtgg
    23281 tggacatgat attgcgtatg ctcaatattt agcaacacgt aaagtctatc caacacaatc
    23341 tattggtgtg ataaatattg atgcgcattt tgatacacgt gctgaacaac aatctacatc
    23401 tggaacgagc tttagacaaa ttttagaaga agatgaaaac acagattatt tagtgcttgg
    23461 tattgctcaa ggtggtaata cgcaaagttt atttgattat gctaaagaga aaaagattga
    23521 ttatgtcttt gcagatgaat tattgagtca cgtatcacca acaattaaag atatgattga
    23581 acgttttgta catgaacatg atgtcattat gtttacgatt tgtatggatg tcattgatag
    23641 tgcgtttgcg cctggggtaa gtgcgccggc agtgttaggt ttgtatccac atactgttct
    23701 tgaattagca aaacgtatta ttccaagtga taaggtgtct tcagttagta ttgctgagat
    23761 gaatccaaca tatgacgctg acaatagaac tgctaagctc gttgctaatt tagtgcatca
    23821 ttttttaaaa taattaaaaa tatttatatg tgacgagatt tgcattgcag ggcagaatgt
    23881 gagtatttct taatagaagt tatattaacg ttgatatatt tgaaaaataa ttgcagtaag
    23941 aagactctta agttgtagtc atcttactgc ttatttatgt ttgttgaaat agcaaaaata
    24001 acaactgta
//
LOCUS       DALAGD010000039        23920 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.039, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000039 DALAGD010000000
VERSION     DALAGD010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 23920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23920
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.039"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(<1..1143)
                     /locus_tag="O2701_001256"
     CDS             complement(<1..1143)
                     /locus_tag="O2701_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCZ4293159.1"
                     /translation="MKNAFKLFKMDLKKVAKTPAVWIILAGLAILPSFYAWFNLWAMW
                     DPYGNTGHIKVAVVNEDKGDTIRGKKVNVGNTMVNTLKKNKSFDWQFVSREKADHEIK
                     MGKYFAGIYIPSKFTHEITGTLRKQPQKADVEFKVNQKINAVASKLTDTGSSVVVEKA
                     NEQFNKTVTRALLEEANKAGLTIEENVPTINKIKNAVYSADKALPKINDFANKIVYLN
                     NHQADLDKYANDFRKLGNYKGDILDAQKKLNEVNGAIPQLNEKAKLILALNNYMPKIE
                     KALNFAADDVPAQFPKINQGLNIASQGIDQANGQLNDAKGFVTQVRSRVGDYQDAIRR
                     AQDLNRRNQQQIPQNSAANNETSNSAPAAGNGVASTPPSAPSGDTAP"
     gene            1354..3213
                     /locus_tag="O2701_001257"
     CDS             1354..3213
                     /locus_tag="O2701_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HCZ4293160.1"
                     /translation="MPKNKILIYLLSTTLVLPTLVSPTAYADTPQKDTTAKTTSHDSK
                     KSNDDETSKDTTSKDIDKADKNNTSNQDNNDKKFKTIDDSTSDSNNIIDFIYKNLPQT
                     NINQLLTKNKYDDNYSLTTLIQNLFNLNSDISDYEQPRNGEKSTNDSNKNSDNSIKND
                     TDTQSSKQDKADNQKAPKSNNTKPSTSNKQPNSPKPTQPNQSNSQPASDDKANQKSSS
                     KDNQSMSDSALDSILDQYSEDAKKAQKDYASQSKKDKNEKSNTKNPQLPTQDELKHKS
                     KPAQSFNNDVNQKDTRATSLFETDPSISNNDDSGQFNVVDSKDTRQFVKSIAKDAHRI
                     GQDNDIYASVMIAQAILESDSGRSALAKSPNHNLFGIKGAFEGNSVPFNTLEADGNQL
                     YSINAGFRKYPSTKESLKDYSDLIKNGIDGNRTIYKPTWKSEADSYKDATSHLSKTYA
                     TDPNYAKKLNSIIKHYQLTQFDDERMPDLDKYERSIKDYDDSSDEFKPFREVSDSMPY
                     PHGQCTWYVYNRMKQFGTSISGDLGDAHNWNNRAQYRDYQVSHTPKRHAAVVFEAGQF
                     GADQHYGHVAFVEKVNSDGSIVISESNVKGLGIISHRTINAAAAEELSYITGK"
     gene            complement(3480..4040)
                     /locus_tag="O2701_001258"
     CDS             complement(3480..4040)
                     /locus_tag="O2701_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCZ4293161.1"
                     /translation="MSRKTALLVLDMQEGIASSVPRIKNIIKANQRAIEAARQHRIPV
                     IFIRLVLDKHFNDVSSSNKVFSTIKAQGYAITEADASTRILEDLAPLEDEPIISKRRF
                     SAFTGSYLEVYLRANDINHLVLTGVSTSGAVLSTALESVDKDYYITVLEDAVGDRSDD
                     KHDFIIEQILSRSCDIESVESWKSSL"
     gene            complement(4208..6115)
                     /gene="sasF"
                     /locus_tag="O2701_001259"
     CDS             complement(4208..6115)
                     /gene="sasF"
                     /locus_tag="O2701_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasF"
                     /protein_id="HCZ4293162.1"
                     /translation="MAKYRGKPFQLYVKLSCSTMMATSIILTNILPYDAQAASEKDTE
                     ITKEILSKQDLLDKVDKAIRQIEQLKQLSASSKEHYKAQLNEAKTASQIDEIIKRANE
                     LDSKDNKSSHTEMNGQSDIDSKLDQLLKDLNEVSSNVDRGQQSGEDDLNAMKNDMSQT
                     ATTKHGEKDDKNDEAMVNKALEDLDHLNQQIHKSKDASKDTSEDPAVSTTDNNHEVAK
                     TPNNDGSGHVVLNKFLSNEENQSHSNRLTDKLQGSDKINHAMIEKLAKSNASTQHYTY
                     HKLNTLQSLDQRIANTQLPKNQKSDLMSEVNKTKERIKSQRNIILEELARTDDKKYAT
                     QSILESIFNKDEAVKILKDIRVDGKTDQQIADQITRHIDQLSLTTSDDLLTSLIDQSQ
                     DKSLLISQILQTKLGKAEADKLAKDWTNKGLSNRQIVDQLKKHFASTGDTSSDDILKA
                     ILNNAKDKKQAIETILATRIERQKAKLLADLITKIETDQNKIFNLVKSALNGKADDLL
                     NLQKRLNQTKKDIDYILSPIVNRPSLLDRLNKNGKTTDLNKLANLMNQGSDLLDSIPD
                     IPTPKPEKTLTLGKGNGLLSGLLNADGNVSLPKAGETIKEHWLPISVIVGAMGVLMIW
                     LSRRNKLKNKA"
     gene            complement(6357..7715)
                     /gene="gtfB"
                     /locus_tag="O2701_001260"
     CDS             complement(6357..7715)
                     /gene="gtfB"
                     /locus_tag="O2701_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HCZ4293163.1"
                     /translation="MIKLFDYFNDHSRKLYESFKASKLEEDLTIVLNDNGFLPDDVIS
                     PYQFFADNHNTENMKPRFFNQVTVPAFWEIKGNNNSATINDMGRLRGKIFYQSGERPR
                     IVSRVEWFDDQQRVRFVDYYSKNGIKFAQTVYDLNRKAILKKYMTAEGKEVIYENFVT
                     SDVILDWQGKSYFFPSKLAFALFFIKQLEITEHHFVINSLALPFSVLYNLPSKGSDVL
                     VWQEHCNGNVPGNMQLMCKGDMKRHCNIIIPDKNEYETMLNIADAKVQSRILQGGYLY
                     NYRSRNRYTKEIVILTNSDQLRNIKVLVETLPDFKFHIAAITEMSDKLMQLDQYANVH
                     LYPSINIDRVNELYQLCDIYLDINEGNEILNAVEQAFDYELLILGYRQTAHHAKVTLS
                     EHLFEHNDEITMESKDQLIQMLESLKDQQQFRDALLAQKAHAHEISREQFEQVFKQAL
                     ES"
     gene            complement(7708..9216)
                     /gene="gtfA"
                     /locus_tag="O2701_001261"
     CDS             complement(7708..9216)
                     /gene="gtfA"
                     /locus_tag="O2701_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HCZ4293164.1"
                     /translation="MTIYNINFGIGWASSGVEYAQAYRAKLLRRTNQDTKFVFLDFIQ
                     SENIQTLTQNIGFKDQEVIWLYQYFSDISLAPTTYTLDDIERELGHDISFRERNGKIV
                     RLYFNGKSSFVTCYLQNEQKDIVDRAEFVINSMLVRKDFYSYTRIFSEYYAPADNKAK
                     LYMRQFYNQDGSIAYNEYIDGDSSIYVFEDAVLYNKTEFIAYFLQRLNLTRDDIVILD
                     RASDIGQAVLQHKGDSKVGVVIHADHYSNNMMSEQHILWNNYYEYQFSKAKYIDFFIT
                     ATDIQNHMVCRQFEQYQGYRPRVYTIPVGSIDALSYPTLSRKPYAMISASRLANEKHI
                     DWLVKAVIVAKRQVPELTFDIYGEGSEKTRLRKIIDTHRAQDYIRLLGHVKLDEIYND
                     YELFLSASTSEGFGLTLMEAVGSGLGMIGLNVNYGNPTFIRDGENGYLVPFDTDEDSV
                     DDVIAKLAHAIVMYFNNGPQTPHDISYEVAQQFMTQDIILKWETLVQEVLHD"
     gene            complement(9234..11624)
                     /gene="secA2"
                     /locus_tag="O2701_001262"
     CDS             complement(9234..11624)
                     /gene="secA2"
                     /locus_tag="O2701_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HCZ4293165.1"
                     /translation="MKHKLDVTINELRLKSIRKIVKRINTWSDEVKSYSDDALKQKTI
                     EFKERLASGVDTLDTLLPEAYAVAREASWRVLGMYPKEVQLIGAIVLHEGNIAEMQTG
                     EGKTLTATMPLYLNALSGKGTYLITTNDYLAKRDFEEMQPLYEWLGLTASLGFVDIVD
                     YEYQKGEKRNIYEHDIIYTTNGRLGFDYLIDNLADSAEGKFLPQLNYGIIDEVDSIIL
                     DAAQTPLVISGAPRLQSNLFHIVKEFVDTLIEDVHFKMKKTKKEIWLLNQGIEAAQSY
                     FNVEDLYSEQAMVLVRNINLALRAQYLFESNVDYFVYNGDIVLIDRITGRMLPGTKLQ
                     AGLHQAIEAKEGMEVSTDKSVMATITFQNLFKLFESFSGMTATGKLGESEFFDLYSKI
                     VVQVPTDKAIQRIDEPDKVFRSVDEKNIAMIHDIVELHETGRPVLLITRTAEAAEYFS
                     KVLFQMDIPNNLLIAQNVAKEAQMIAEAGQIGSMTVATSMAGRGTDIKLGEGVEALGG
                     LAVIIHEHMENSRVDRQLRGRSGRQGDPGSSCIYISLDDYLVKRWSDSNLAENNQLYS
                     LDAQRLSQSNLFNRKVKQIVVKAQRISEEQGVKAREMANEFEKSISIQRDLVYEERNR
                     VLEIDDAENQDFKALAKDVFEMFVNEEKVLTKSRVVEYIYQNLSFQFNKDVACVNFKD
                     KQAVVTFLLEQFEKQLALNRKNMQSAYYYNIFVQKVFLKAIDSCWLEQVDYLQQLKAS
                     VNQRQNGQRNAIFEYHRVALDSFEVMTRNIKKRMVKNICQSMITFDKEGMPVIHFP"
     gene            complement(11614..12573)
                     /gene="asp3"
                     /locus_tag="O2701_001263"
     CDS             complement(11614..12573)
                     /gene="asp3"
                     /locus_tag="O2701_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HCZ4293166.1"
                     /translation="MLKNKTFKVTWNYISQTTFMYGSKVSFSNGEVTFINPLMPSGLP
                     IHEWLMLKQFSKYKSAPSLPILRRGQHYKLHFDFDATPAGSVYFIIIFYNKNGTKLST
                     EIVKSNSITIQYPDEAYAYKIKMMNAASTSLIFRCLTITEMTHQYDLEYKSMRVTKIG
                     DDQYGNDMINVIIAEPSDTYPTISNDFLKLFGHVWLVERWMDDNIEGNIKQLKNDLQS
                     QDTLKAINLISYGSKSNVFATYVAQHLGCKVYRTSHEDDDLKGWLTEHVPGNHELKDT
                     NVEVYFKEEQDKHLNYMSRLMNPVRFLDYLDVSKLNGGEVNET"
     gene            complement(12551..14119)
                     /gene="asp2"
                     /locus_tag="O2701_001264"
     CDS             complement(12551..14119)
                     /gene="asp2"
                     /locus_tag="O2701_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HCZ4293167.1"
                     /translation="MPRKFRVLQIGGDDLEPIFQHKKGVSWDYFDIGLFEFDSGYVEA
                     IEAIVEAEGRFDFIYIQAPYSETLTNLLQMISEPYNTYVDESFWSVEYEQDENVQKYV
                     VQPLHYRNIEERNNKLEAVSFSGQYGDKVSPKLALVHPNFKGDVVYQGNSELTLSGEF
                     GKEFKPIASWQNNLVYDKDKVIQIWPEFDIDGAVELQYTFRLIQTGADGALIEQIVLT
                     DDMLDSPLEIPAKPFDAYISVTVKARGNGTVHLGPIHKRWSRLDMGQFLLGGSRFVDS
                     QRQEFIYYFHPGDMKPPLNVYFSGYRTAEGFEGYYMMKRMNAPFLLIGDPRVEGGSFY
                     IGSSEYEQGIINVIDETLEKLNFKSHELILSGLSMGSFGALYYGAQLNPQAIIVGKPL
                     VNIGTIAEHMRLLRPEEFGTALDVLVSNEGDTSQASIQALNQKFWQTFQKKSLSQTVF
                     AIAYMQHDDYDPHAFQELLPVLTAHQARVMNRSIPGRHNDDSPTIASWFVNFYNIILE
                     DKFGRVQHAEKQNI"
     gene            complement(14109..15662)
                     /gene="asp1"
                     /locus_tag="O2701_001265"
     CDS             complement(14109..15662)
                     /gene="asp1"
                     /locus_tag="O2701_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HCZ4293168.1"
                     /translation="MKYFIPAWYDDQRWWQDTTVPYYQLQNKTEFDDMISLMGMHLEN
                     DLDYQLIVLNHAPNLRTFLHRYDLYETKYSSVFDEIQGFSHHAPQAINYHHLKWPDDV
                     EFVYTPYLLKCVTSEQTYTNIYFSQEGYSIWFEEFERDQLQRRYIFDDRGYLSAIRYF
                     DDQGEASYQEYLTINGDCVLHEDLKNGRVTVSKRYQHHYQQTEYNNMAQLIEEKFQAM
                     IAQQIHEDDHVIVASDARHNRQIANHIPAKSLSYSFFKNRNETVSDEEYQSIVKNAHL
                     IVDSVQLERDLISHQEKYQRENTMIRITPFETRQSPNISSQLMETFIGVWIDGMSDAD
                     LQQMMQRLVDYIAQEDYYRLILLSRHQNDIPMWLRECITSVNEEYQAKQNADVNVSAL
                     MTPEDQDDIIAVKTIHAEHDVVEALRTLRLVIDMSKEPDLYLQISAISAGIPQINGQQ
                     TDYVSDYDNGRIINTVDELDDALNYYLFYLKNWNYAYAYSLKLIDAYASKNIINQLDE
                     LIEGENDAT"
     gene            complement(15673..16884)
                     /gene="secY2"
                     /locus_tag="O2701_001266"
     CDS             complement(15673..16884)
                     /gene="secY2"
                     /locus_tag="O2701_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HCZ4293169.1"
                     /translation="MLKLLQQYEYKIIYKRMLYTCFILFIYILGTNISIVSYNDMQVK
                     HESFFKIAISNMGGDVNTLNIFTLGLGPWLTSMIILMLISYRNMDKYMKQTSLEKHYK
                     ERILTLILSVIQSYFVIHEYVSKERVHQDNIYLTILILVTGTMLLVWLADKNSRYGIA
                     GPMPIVMVSIIKSMMHQKMEYIDASHIVIALLIILVIITLFILLFIELVEVRIPYIDL
                     MNVSATNMKSYLSWKVNPAGSITLMMSISAFVFLKSGIHFILSMFNKSISDDMPMLTF
                     DSPVGISVYLVIQMLLGYFLSRFLINTKQKSKDFLKSGNYFSGVKPGKDTERYLNYQA
                     RRVCWFGLALVTVIIGIPLYFTLFVPHLSTEIYFSVQLIVLVYISINIAETIRTYLYF
                     DKYKPFLNQYW"
     gene            complement(17014..23829)
                     /gene="sasA"
                     /locus_tag="O2701_001267"
     CDS             complement(17014..23829)
                     /gene="sasA"
                     /locus_tag="O2701_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-rich repeat glycoprotein adhesin SasA"
                     /protein_id="HCZ4293170.1"
                     /translation="MSKRQKAFHDSLANEKTRVRLYKSGKNWVKSGIKEIEMFKIMGL
                     PFISHSLVSQDNQSISKKMTGYGLKTTAVIGGAFTVNMLHDQQAFAASDAPLTSELNT
                     QSETVGNQNSTTIEASTSTADSTSVTKNSSSVQTSNSDTVSSEKSEKVTSTTNSTSNQ
                     QEKLTSTSESTSSKNTTSSSDTKSVASTSSTEQPINTSTNQSTASNNTSQSTTPSSVN
                     LNKTSTTSTSTAPVKLRTFSRLAMSTFASAATTTAVTANTITVNKDNLKQYMTTSGNA
                     TYDQSTGIVTLTQDAYSQKGAITLGTRIDSNKSFHFSGKVNLGNKYEGHGNGGDGIGF
                     AFSPGVLGETGLNGAAVGIGGLSNAFGFKLDTYHNTSKPNSAAKANADPSNVAGGGAF
                     GAFVTTDSYGVATTYTSSSTADNAAKLNVQPTNNTFQDFDINYNGDTKVMTVKYAGQT
                     WTRNISDWIAKSGTTNFSLSMTASTGGATNLQQVQFGTFEYTESAVTQVRYVDVTTGK
                     DIIPPKTYSGNVDQVVTIDNQQSALTAKGYNYTSVDSSYASTYNDTNKTVKMTNAGQS
                     VTYYFTDVKAPTVTVGNQTIEVGKTMNPIVLTTTDNGTGTVTNTVTGLPSGLSYDSAT
                     NSIIGTPTKIGQSTVTVVSTDQANNKSTTTFTINVVDTTAPTVTPIGDQSSEVYSPIS
                     PIKIATQDNSGNAVTNTVTGLPSGLTFDSTNNTISGTPTNIGTSTISIVSTDASGNKT
                     TTTFKYEVTRNSMSDSVSTSGSTQQSQSVSTSKADSQSASTSTSGSIVVSTSASTSKS
                     TSVSLSDSVSASKSLSTSESNSVSSSTSTSLVNSQSVSSSMSDSASKSTSLSDSISNS
                     SSTEKSESLSTSTSDSLRTSTSLSDSLSMSTSGSLSKSQSLSTSISGSSSTSASLSDS
                     TSNAISTSTSLSESASTSDSISISNSIANSQSASTSKSDSQSTSISLSTSDSKSMSTS
                     ESLSDSTSTSGSVSGSLSIAASQSVSTSTSDSMSTSEIVSDSISTSGSLSASDSKSMS
                     VSSSMSTSQSGSTSESLSDSQSTSDSDSKSLSQSTSQSGSTSTSTSTSASVRTSESQS
                     TSGSMSASQSDSMSISTSFSDSTSDSKSASTASSESISQSAFTSTSGSVSTSTSLSTS
                     NSERTSTSMSDSTSLSTSESDSISESTSTSDSISEAISASESTFISLSESNSTSDSES
                     QSASAFLSESLSESTSESTSESVSSSTSESTSLSDSTSESGSTSTSLSNSTSGSTSIS
                     TSTSISESTSTFKSESVSTSLSMSTSTSLSDSTSLSTSLSDSTSDSKSDSLSTSMSTS
                     DSISTSKSDSISTSTSLSGSTSESESDSTSSSESKSDSTSMSISMSQSTSGSTSTSTS
                     TSLSDSTSTSLSLSASMNQSGVDSNSASQSASNSTSTSTSESDSQSTSSYTSQSTSQS
                     ESTSTSTSLSDSTSISKSTSQSGSVSTSASLSGSESESDSQSISTSASESTSESASTS
                     LSDSTSTSNSGSASTSTSLSNSASASESDLSSTSLSDSTSASMQSSESDSQSTSASLS
                     DSLSTSTSNRMSTIASLSTSVSTSESGSTSESTSESDSTSTSLSDSQSTSRSTSASGS
                     ASTSTSTSDSRSTSASTSTSMRTSTSDSQSMSLSTSTSTSMSDSTSLSDSVSDSTSDS
                     TSASTSGSMSVSISLSDSTSTSTSASEVMSASISDSQSMSESVNDSESVSESNSESDS
                     KSMSGSTSVSDSGSLSVSTSLRKSESVSESSSLSCSQSMSDSVSTSDSSSLSVSTSLR
                     SSESVSESDSLSDSKSTSGSTSTSTSGSLSTSTSLSGSESVSESTSLSDSISMSDSTS
                     TSDSDSLSGSISLSGSTSLSTSDSLSDSKSLSSSQSMSGSESTSTSVSDSQSSSTSNS
                     QFDSMSISASESDSMSTSDSSSISGSNSTSTSLSTSDSMSGSVSVSTSTSLSDSISGS
                     TSVSDSSSTSTSTSLSDSMSQSQSTSTSASGSLSTSISTSMSMSASTSSSQSTSVSTS
                     LSTSDSISDSTSISISGSQSTVESESTSDSTSISDSESLSTSDSDSTSTSTSDSTSGS
                     TSTSISESLSTSGSGSTSVSDSTSMSESNSSSVSMSQDKSDSTSISDSESVSTSTSTS
                     LSTSDSTSTSESLSTSMSGSQSISDSTSTSMSGSTSTSESNSMHPSDSMSMHHTHSTS
                     TSRLSSEATTSTSESQSTLSATSEVTKHNGTPAQSEKRLPDTGDSIKQNGLLGGVMTL
                     LVGLGLMKRKKKKDENDQDDSQA"
BASE COUNT         7177 a         4549 c         3763 g         8431 t
ORIGIN      
        1 tggtgcagta tcgccacttg gtgcacttgg tggcgttgat gctacaccat taccagctgc
       61 aggtgcacta tttgatgttt cgttgttcgc cgcgctattt tgaggaatct gttgctggtt
      121 tcttcgattt aaatcttgcg cgcgtcgaat tgcatcttga taatcaccga ctctacttct
      181 aacttgtgtg acgaagcctt tggcatcatt taactgtcca ttagcttgat caataccttg
      241 actcgcaatg ttaagtcctt gattaatttt agggaactgc gctggcacgt catcagctgc
      301 aaaatttaac gctttttcaa ttttcggcat ataattattt aaagctaata tcaacttagc
      361 cttttcatta agttgcggaa tagcaccatt gacttcgttt aattttttct gagcatctaa
      421 aatatcacct ttataatttc ctagttttct aaaatcattg gcatatttat ctaaatccgc
      481 ttggtggtta ttcaaatata caattttatt cgcaaagtca ttaatcttag gtaaagcttt
      541 atctgctgaa tataccgcat tttttatctt gttaattgtc ggcacatttt cttcaatagt
      601 taaacctgct ttgttagctt cttctaataa tgctcgagtt actgttttat taaattgttc
      661 attcgctttt tcaacgacaa ctgacgaacc agtatctgtt agcttagacg caacagcgtt
      721 aatcttctga ttcaccttaa attctacatc tgctttttga ggctgcttac gtagtgtccc
      781 tgtaatttca tgtgtaaact tagatgggat gtaaatacct gcaaaatatt tacccatttt
      841 tatctcatga tcagctttct ctctacttac aaactgccaa tcaaaacttt tatttttctt
      901 gagtgtatta accatcgtat taccgacatt aacttttttc cctctgattg tgtcgccttt
      961 atcttcatta acgactgcga ccttgatgtg tcccgtgttg ccatatggat cccacattgc
     1021 ccataagtta aaccaagcgt agaacgatgg caaaatagct aagcctgcta agataatcca
     1081 cacagctggc gtcttagcta ctttcttcag atccatttta aataatttaa atgcgttctt
     1141 cattgtcaca ctcctatgta ggaattattc atatttttta tatatttttt gtaaattaat
     1201 ttatttttgc gttgtgaatt agtataatca atttactgga agatatttag tcgattgata
     1261 cctatcaact attttcagca tacgataaat tataacaaat catagtttat tatcacactt
     1321 aattattata tttttcaagg gagaatacga aatatgccta aaaataaaat tttaatttat
     1381 ttgctatcaa ctacgctcgt attacctact ttagtttcac ctaccgctta tgctgataca
     1441 cctcaaaaag atactacagc taagacaaca tctcatgatt caaaaaaatc taatgacgat
     1501 gaaacttcta aggatactac aagtaaagat attgataaag cagacaaaaa taatacaagt
     1561 aaccaagaca ataacgacaa aaaatttaaa actatagacg acagcacttc agactctaac
     1621 aatatcattg attttattta taagaattta ccacaaacca atataaacca attgctaacc
     1681 aaaaataaat acgatgataa ttactcatta acaactttaa tccaaaactt attcaattta
     1741 aattcggata tttctgatta cgaacaacct cgtaatggcg aaaagtcaac aaatgattcg
     1801 aataaaaaca gtgacaatag catcaaaaat gacactgata cgcaatcatc taaacaagat
     1861 aaagcagaca atcaaaaagc acctaaatca aacaatacaa aaccaagtac atctaataag
     1921 caaccaaatt cgccaaagcc aacacaacct aatcaatcaa atagtcaacc agcaagtgac
     1981 gataaagcaa atcaaaaatc ttcatcgaaa gataatcaat caatgtcaga ttcggcttta
     2041 gactctattt tggatcaata cagtgaagat gcaaagaaag cacaaaaaga ttatgcatct
     2101 caatctaaaa aagacaaaaa tgaaaaatct aatacaaaga atccacagtt accaacacaa
     2161 gatgaattga aacataaatc taaacctgct caatcattca ataacgatgt taatcaaaag
     2221 gatacacgtg caacatcatt attcgaaaca gatcctagta tatctaacaa tgatgatagc
     2281 ggacaattta acgttgttga ctcaaaagat acacgtcaat ttgtcaaatc aattgctaaa
     2341 gatgcacatc gcattggtca agataacgat atttatgcgt ctgtcatgat tgcccaagca
     2401 atcttagaat ctgactcagg tcgtagtgct ttagctaagt caccaaacca taatttattc
     2461 ggtatcaaag gtgcttttga agggaattct gttcctttta acacattaga agctgatggt
     2521 aatcaattgt atagtattaa tgctggattc cgaaaatatc caagcacgaa agaatcacta
     2581 aaagattact ctgaccttat taaaaatggt attgatggca atcgaacaat ttataaacca
     2641 acatggaaat cggaagccga ttcttataaa gatgcaacat cacacttatc taaaacatat
     2701 gctacagatc caaactatgc taagaaatta aacagtatta ttaaacacta tcaattaact
     2761 cagtttgacg atgaacgcat gccagattta gataaatatg aacgttctat caaggattat
     2821 gatgattcat cagatgaatt caaacctttc cgtgaggtat ctgatagtat gccatatcca
     2881 catggccaat gtacttggta cgtatataac cgtatgaaac aatttggtac atctatctca
     2941 ggtgatttag gtgatgcaca taattggaat aatcgagctc aataccgtga ttatcaagta
     3001 agtcatacac caaaacgtca tgctgctgtt gtatttgagg ctggacaatt tggtgcagat
     3061 caacattacg gtcatgtagc atttgttgaa aaagttaaca gtgatggttc tatcgttatt
     3121 tcagaatcca atgttaaagg attaggtatc atttctcata gaactatcaa cgcagctgcc
     3181 gctgaagaat tatcatatat tacaggtaaa taagtattat attaaacccg taaaatttat
     3241 aagtataaac aaggagttcg gacttaaaca tatttctgtt cataagtctg atttcttatt
     3301 caattaaacc cgaggcattc agttcgaacg cctcgggtcg ttttatataa atatattatt
     3361 ttatgttcaa atgttcctca tcatatccgt ttcaattgcc atctcacaca ttttataaat
     3421 atgagcaaat gtacttattc tcaaacatta ctgccgagct ttaattgacg ttatattaac
     3481 tataaactac ttttccatga ctctacggat tcaatgtcac atgagcgtga taaaatttgt
     3541 tcaataataa agtcatgttt atcatctgat ctatcaccaa cagcatcttc taaaacagta
     3601 atataatagt ctttatctac actttctaat gccgtgctca atacagctcc acttgtagag
     3661 acacccgtta atactaaatg attaatatca tttgcacgta aataaacttc caagtaacta
     3721 cctgtaaatg cgctaaagcg tcgcttagaa ataatcggct catcttctag tggtgctaaa
     3781 tcttcaagta ttcgtgtaga tgcatctgct tcagtaatcg catatccttg agctttaatt
     3841 gttgaaaaca ctttattact cgaggagaca tcattaaaat gcttatctaa cactaaacgt
     3901 atgaaaatga ctggtattcg atgttgtctt gctgcttcaa ttgctctctg attcgcttta
     3961 ataatatttt ttattctagg tacactactc gctatacctt cttgcatatc caaaactaat
     4021 agcgccgttt ttcgagacat cttcattctc ctttacttgt gtagttctaa gtcgttaaat
     4081 tcattataac gttaaaatga tggacaatct attcattgca ttttgcatat acttcacaat
     4141 aaattaaggg gaaataagac gtcttatata cttaaaaaaa tatatagatg ctcttccccc
     4201 aatataatta tgctttattt ttcaacttat tgcgtcgtga taaccaaatc attagtacac
     4261 ccattgcacc aacaattaca gatatcggca accaatgttc ttttatcgtt tcccccgctt
     4321 taggcaaaga tacattacca tcagcattta ataatccact taacaatcca ttacctttac
     4381 caagtgttaa cgtcttttct ggctttggtg tgggtatatc tggaatactg tctaataaat
     4441 ctgatccttg attcattaaa tttgctaact tatttaaatc tgtcgttttc ccatttttat
     4501 tcaatcgatc tagtaaactt ggacgattta ctattggtga taaaatataa tctatatctt
     4561 ttttcgtttg attgagtctc ttttgtaaat tcaataaatc atccgcttta ccattcaatg
     4621 ccgatttaac taaattaaaa attttatttt gatctgtttc tattttagta attaaatctg
     4681 ccagtaattt tgccttttgt ctttctatac gtgttgctaa aatcgtttca attgcttgtt
     4741 ttttatcttt ggcattattc aaaattgctt ttaatatatc atctgaagac gtgtcgccag
     4801 ttgatgcaaa atgtttcttc aattggtcaa cgatttggcg atttgataat cctttattcg
     4861 tccaatcttt agccaattta tctgcttcag cttttcctaa tttcgtttgt aaaatttgag
     4921 aaatcaatag cgacttatct tgtgattgat caatcaatga cgttaataaa tcatcactcg
     4981 ttgtcagaga taattgatca atatgacgag taatttgatc tgcaatttgt tgatctgttt
     5041 taccatcaac acgtatatct tttagaattt taactgcctc gtctttatta aatatacttt
     5101 ctaaaatgct ttgtgtagca tactttttat catcagtacg tgcaagttct tccaaaataa
     5161 tatttcgttg actttttata cgctctttcg tcttatttac ttcgctcatt aagtctgatt
     5221 tttgattttt aggaagttgc gtatttgcaa tacgttgatc taaagattgt aacgtattca
     5281 gtttatgata tgtgtaatgt tgcgttgagg cattactttt agctaatttt tcaatcatag
     5341 catgattaat tttatcgctt ccttgtaatt tatcagtgag tcgattacta tggctttgat
     5401 tctcttcatt tgaaaggaat ttatttaaca caacatgtcc agaaccatca ttatttggcg
     5461 ttttagctac ttcatgatta ttatctgttg tagacactgc tggatcttcc gatgtatctt
     5521 tcgatgcatc tttcgatttg tgtatttgct gattcaaatg gtctaggtct tctaacgcct
     5581 tatttaccat tgcttcatca tttttatcat ctttttctcc atgttttgtt gtagccgttt
     5641 gtgacatatc atttttcatt gcattaagat cgtcctcgcc actttgttga cccctatcaa
     5701 catttgaaga aacctcattt aaatctttaa gcaattgatc taatttactg tctatatcac
     5761 tttgaccgtt catttcagtg tgagaacttt tattgtcttt gctatccaac tcattagctc
     5821 gttttatgat ttcatctatt tgcgatgctg ttttcgcttc atttagttgt gctttataat
     5881 gttctttaga tgaagccgat aactgtttta attgctcaat ttgacgaatt gccttgtcaa
     5941 ctttgtctaa taaatcttgc ttagataata tctcttttgt aatttcagta tccttttcag
     6001 atgcagcttg ggcatcgtac ggcaagatat tcgttaaaat gatacttgtc gccatcattg
     6061 tcgaacacga taactttaca tataattgaa acggtttccc tcgatattta gccatcaaca
     6121 tactccttcc tcacttactt ccttcaaaga attacatact attatatacc tgtttacaag
     6181 aaatttacac ttatctatct agttattgtt gttagtaatt atctacttat tacttagctt
     6241 atatttaagt aaacaaaaca agcatgacgt aatatcatat tgtccatgtc gctaacatca
     6301 tattacgtca aatcttttat attaaatgat gttttatttt agactgcttt ttccttttag
     6361 ctttcgagcg cctgtttaaa aacttgctcg aattgttcac gcgagatttc gtgtgcatgt
     6421 gctttttgtg ctaataaagc atctcgaaac tgttgttgat ctttcaaact ttctaacatt
     6481 tgtattaatt ggtctttact ttccattgtt atctcatcat tatgctcaaa taagtgctct
     6541 gataatgtta ctttagcatg gtgtgcggtt tgacgataac ctaaaatcaa caactcatag
     6601 tcaaacgctt gttccaccgc atttaaaatt tcattaccct cattgatatc aagataaata
     6661 tcacataact ggtatagttc atttaccctg tcaatattaa tagatgggta taaatgcaca
     6721 ttagcatatt gatcaagttg cattagctta tcagacatct ctgtaatagc agcgatgtga
     6781 aacttaaaat ctggtaaagt ttcaaccaat accttgatgt tacgaagttg atccgagtta
     6841 gttaatatta caatttcttt agtatatcta ttacgactac gatagttata tagatatccg
     6901 ccttgtaaaa tacgagattg aacctttgcg tctgctatat tgagcatcgt ttcatattcg
     6961 tttttatctg gaataataat attacaatgt cgtttcatat cacctttaca catcaattgc
     7021 atatttcccg ggacattacc attacagtgt tcttgccata ccaaaacatc actacctttt
     7081 gatggcaaat tatataacac tgaaaatggt agggctagtg agttaataac gaaatgatgt
     7141 tccgtaattt caagttgctt gataaaaaat aatgcgaatg cgagctttga agggaaaaag
     7201 taagacttcc cttgccaatc caatatgaca tcagatgtta caaaattttc ataaatcact
     7261 tctttacctt ctgctgtcat atatttcttc aagatcgctt tacgatttaa atcgtaaaca
     7321 gtttgtgcaa atttaatacc attcttagaa taataatcga caaatcggac acgttgttgg
     7381 tcatcaaacc attcgacacg actaacaatt ctagggcgct ctccactttg ataaaatatt
     7441 ttacctcgta gacgtcccat atcattaatt gtagccgaat tgttgttacc tttaatttcc
     7501 caaaaagctg gtacagtaac ctgattaaaa aatcgtggtt tcatattttc tgtattatga
     7561 ttatctgcaa aaaattgata cggtgatata acatcgtccg gtaaaaagcc attgtcattg
     7621 agtacaattg ttaaatcttc ttccaactta ctggctttaa aagactcata taactttcgt
     7681 gaatgatcgt taaagtaatc aaataattta atcatgtagc acctcttgaa ctaatgtttc
     7741 ccattttaaa ataatatctt gagtcataaa ttgctgtgcc acttcataag agatgtcatg
     7801 tggtgtctgg ggaccattgt taaaatacat tacaatggca tgagctagtt ttgcgataac
     7861 atcatccaca ctatcttcgt cggtatcaaa aggtaccaag tagccatttt ccccatctcg
     7921 aataaaggtt gggttaccat aattcacatt taatccaatc atacctagtc ctgagcctac
     7981 cgcttccatt agtgttaacc caaaaccttc gctagttgat gcagaaagaa ataactcata
     8041 atcattataa atttcatcaa gtttaacatg ccctagtaaa cgaatataat cttgtgcgcg
     8101 gtgtgtatca ataattttac gcagtcgcgt cttctcgcta ccttctccat aaatatcaaa
     8161 tgttaattct ggcacttgtc gtttagccac gataaccgct ttgacaagcc aatcaatatg
     8221 tttctcattc gctaaacgag atgcactaat catcgcatat ggctttcttg ataatgttgg
     8281 atatgataat gcatcaatgc ttcccacagg aatagtataa acacgtgggc gataaccttg
     8341 atattgctca aattgtcgac aaaccatatg attttgaata tctgttgctg taataaagaa
     8401 atcaatgtat ttagcttttg aaaattgata ttcataataa ttgttccata gtatatgctg
     8461 ctcactcatc atattattac tataatgatc agcatgaatc acaacaccaa ctttactatc
     8521 acctttatgc tgcaaaacag cctgaccaat atcagaagcg cggtctaata tgacaatatc
     8581 gtctcgggtt aaattcaatc gttgtaaaaa gtatgcaata aattccgttt tgttatacaa
     8641 caccgcatct tcaaacacat atatagagct gtctccatca atatattcgt tataagcgat
     8701 ggaaccatct tgattataaa attgtcgcat atataatttc gctttattat cagctggtgc
     8761 ataatactca gaaaatatgc gcgtataact ataaaaatct ttacgtacta acatactatt
     8821 aattacaaat tctgcacgat ccacaatatc tttttgttca ttttgcagat aacatgttac
     8881 aaatgatgat ttcccattaa aatataggcg gactatctta ccatttcttt ctctaaaact
     8941 aatgtcatga ccaagctcac gttcaatgtc atctaacgtg tacgttgttg gtgctaaaga
     9001 aatatcacta aaatactgat acaaccaaat aacttcttga tctttaaacc caatgttttg
     9061 cgttaatgtc tgtatgttct ctgactgtat aaaatctaaa aacacaaatt tagtgtcttg
     9121 atttgtacgt ctcaataatt tagcacggta agcttgtgca tattcaacac cgctactcgc
     9181 ccagcctata ccaaagttta tattatatat tgtcatgcgc tacccctttt catttatgga
     9241 aaatgtataa ctggcatacc ctctttatca aatgtaatca tgctttgaca aatatttttc
     9301 accattcttt ttttgatatt tcgtgtcata acttcaaatg aatctaaggc aactctatgg
     9361 tattcaaaaa tagcgttacg ttgcccattt tgtcgttgat taacgcttgc ttttaattgt
     9421 tgtaaatagt cgacttgttc taaccaacat gaatcaattg ctttcaaaaa gactttttga
     9481 acgaaaatat tataataata tgcactttgc atgtttttac gattcaaagc taattgcttt
     9541 tcaaattgct ctaataaaaa tgtcactact gcttgcttat ctttaaaatt aacacaagcc
     9601 acatctttat taaattggaa acttaaattt tgataaatat actcgacaac acgcgatttt
     9661 gttagcacct tttcctcatt tacaaacatt tcaaatacat ctttagctaa cgctttaaaa
     9721 tcttgattct cagcatcatc tatttctaaa actcgattgc gttcctcgta tacaagatct
     9781 cgctgtatac taatgctttt ttcaaattca ttagccattt cacgagcttt aaccccttgt
     9841 tcttccgaga tacgctgcgc tttaactaca atttgcttaa ctttgcgatt aaacaaatta
     9901 ctttgcgata atcgttgtgc atctaatgaa tataattgat tattttccgc taaattacta
     9961 tcgctccatc gcttaactaa ataatcatct agtgaaatat atatacaaga tgatcccgga
    10021 tccccttgtc taccagaacg accacgtaat tgcctgtcta cacggctatt ttccatatgt
    10081 tcatgaataa taacagctaa tccacctaat gcttcgacac cttcaccaag tttaatatct
    10141 gtgcctcgac ctgccatact agtcgcaaca gtcatggaac caatttgccc tgcttcagct
    10201 atcatctgcg cttcttttgc aacattttgc gcaatgagta aattattagg aatatccatt
    10261 tggaataata ctttcgaaaa gtattcagcc gcttcagcag ttctcgttat gagtaaaacc
    10321 ggtcgccccg tttcatgaag ttcaactata tcatgaatca tcgcgatgtt tttctcatca
    10381 actgaacgaa acactttatc tggttcatcg atacgttgaa tcgctttatc agttggtact
    10441 tgtacgacta tttttgaata caaatcaaag aactctgatt cgcctaattt tcctgtagct
    10501 gtcatacctg aaaatgattc aaaaagttta aataaattct ggaaggtaat tgttgccata
    10561 acacttttat ctgttgaaac ctccatacct tctttcgctt caatagcttg gtgaagtcca
    10621 gcttgcaact tagttcccgg taacatacga cctgtaatac ggtcaattaa aacaatatca
    10681 ccattatata caaagtaatc gacattagat tcaaacaaat attgtgcgcg cagtgctaaa
    10741 ttaatattac gcactaggac catcgcttgt tcgctatata aatcttcaac attaaagtat
    10801 gattgtgccg cttcaatacc ttgatttaac agccatattt cttttttggt cttcttcatt
    10861 ttaaaatgca cgtcttcaat caatgtatct acaaactctt tcacaatatg aaatagattt
    10921 gattgtaatc ttggtgcacc cgaaataact aatggtgttt gagcagcatc taaaatgatt
    10981 gaatccactt catcaataat accgtaattt aattgtggta aaaatttccc ttccgcacta
    11041 tcagccaaat tatcaattaa ataatcaaaa ccgagacgtc cattagttgt atatataata
    11101 tcatgttcat atatattacg tttttcccct ttttgatact cataatccac aatatcaaca
    11161 aaacctaatg aagcagttaa acctaaccat tcatataatg gttgcatctc ttcaaaatct
    11221 cgcttcgcta aataatcatt cgttgtaatt aaatatgttc cttttcccga aagagcattt
    11281 aaatataaag gcatcgttgc cgttaatgtt ttaccttcgc ctgtttgcat ctccgcaatg
    11341 ttaccttcat gcaatacaat cgctccgatt aactgaactt ctttaggata catacctaat
    11401 actctccagc tcgcttcacg tgccactgca taagcttcag gtaacaatgt atctagtgta
    11461 tcaactcctg atgctaaacg ttctttaaat tctattgtct tttgttttaa cgcatcatca
    11521 gaatatgatt taacttcatc gctccatgta ttgatgcgtt tcactatttt tctaatcgac
    11581 tttagtctta attcgtttat cgtaacatct agtttatgtt tcatttactt ccccaccatt
    11641 cagtttcgat acatctaagt aatctaaaaa tcgtactgga ttcattaaac gtgacatata
    11701 atttagatgt ttgtcttgct cttctttaaa ataaacctcg acatttgtat cttttagttc
    11761 atgatttcct gggacatgtt ctgtaagcca tccttttaaa tcatcatctt catggcttgt
    11821 acgatacact ttgcaaccca aatgctgagc gacataagtt gcaaaaacat ttgactttga
    11881 cccataacta atcaaattaa tagcctttag ggtatcttga ctttgcaaat cattctttag
    11941 ttgcttaata tttccctcga tattgtcgtc catccaacgt tcaacgagcc aaacatgacc
    12001 aaacagtttc aaaaaatcat tcgaaatagt tggataggtg tcagatggtt ctgcaataat
    12061 gacattgatc atatcatttc catattggtc atcgcctatc ttcgtcaccc gcatgctttt
    12121 atactctaaa tcatattgat gcgtcatctc tgtgattgtt aaacatctaa atataagact
    12181 cgtcgatgct gcattcatca tttttatttt ataagcatag gcttcatcag gatattgaat
    12241 cgtaatacta tttgacttta caatctcagt acttagtttt gtgccatttt tattataaaa
    12301 aatgatgata aaatacactg aaccagcagg cgttgcatca aaatcaaaat gcaatttata
    12361 atgctgtcct ctacgcaaaa ttggtaaact tggcgcactt ttatattttg aaaattgctt
    12421 taacatcaac cactcatgaa tcggtaatcc agagggcatc aaaggattta taaaagtcac
    12481 ttcaccattt gaaaatgata ctttagagcc atacataaat gtagtttgtg aaatataatt
    12541 ccaagtaact ttaaatgttt tgtttttcag catgttgaac tctcccaaac ttgtcttcca
    12601 aaataatgtt gtaaaaatta acaaaccaac ttgcaatggt aggtgaatca tcattatgtc
    12661 gcccaggaat actgcgattc atcactcttg cttggtgtgc tgtcaataca ggtaatagct
    12721 cttgaaatgc atgtggatca taatcatcat gttgcatata tgctatggca aaaacagttt
    12781 gtgacaatga tttcttttga aatgtttgcc aaaatttttg atttaatgcc tgtatcgacg
    12841 cttgagatgt atcaccttca ttagacacca ggacgtctaa tgctgtaccg aactcttctg
    12901 gtctaagtaa tcgcatatgt tcagcaatcg ttccaatatt aacaagtggt ttaccaacaa
    12961 taattgcctg aggatttaac tgagcaccat aatacaaggc accaaatgaa cccatagata
    13021 aaccagataa tattaattca tgtgatttaa aattcagctt ttctaatgtc tcgtcaataa
    13081 cattgataat accttgttca tattcagatg aaccgatata aaaactacca ccttcaacac
    13141 gaggatcgcc gataagtaaa aacggtgcat tcatacgttt catcatataa tatccttcga
    13201 aaccttccgc tgttcgataa ccactaaaat atacgtttag tggcggtttc atatcaccag
    13261 ggtggaaata ataaataaat tcctgtcgtt gactatctac gaaacgacta ccaccaagta
    13321 aaaattgacc catgtctaat ctagaccatc gtttgtgtat aggtcctaaa tgtaccgtcc
    13381 cgttcccacg cgccttaaca gttacactta tataagcatc aaatggtttc gcaggtatct
    13441 ctaaaggact gtctaacata tcatcagtca atacgatttg ttcaattaat gcaccatcag
    13501 cgccagtctg aatcaatcta aatgtatatt gcaactcgac cgcaccatca atatcaaatt
    13561 ctggccatat ttgaatgact ttatctttat cgtaaacgag attattttgc caagatgcga
    13621 taggtttaaa ttctttccca aattctccac tcaatgtgag ctctgaatta ccttggtaaa
    13681 cgacatctcc tttaaaattc ggatgcacaa gtgctaactt aggagaaacc ttatctccat
    13741 actgtcctga gaagctaact gcctctaatt tattattacg ttcttcaata ttccggtaat
    13801 gtaatggttg aacaacgtat ttttggacat tttcgtcttg ttcatattca actgaccaaa
    13861 atgattcatc aacatacgta ttgtatggtt cgcttatcat ttgtaataaa ttcgttaatg
    13921 tctccgagta tggtgcttga atatagataa aatcaaagcg cccttctgct tcaacaatcg
    13981 cttcaatagc ctctacataa ccactatcaa attcaaacaa tccaatatcg aagtaatccc
    14041 aactcacacc ttttttgtgt tgaaaaatag gttctaaatc gtctcctcca atttgcaaaa
    14101 ctctaaattt acgtggcatc attttcacct tctattaact catcgagctg attaataata
    14161 ttcttagaag catatgcatc tattaatttt aaagaatagg cgtacgcata attccaattt
    14221 ttcaaataaa ataaataata atttaacgca tcatctaatt catcaactgt atttataata
    14281 cggccattgt cataatcaga gacgtaatct gtttgttgac cattaatttg tggaatccca
    14341 gcgctaattg cactaatttg taaatacaag tcaggttctt ttgacatatc tatcacaagt
    14401 cgcaacgtcc gcaatgcttc tacaacatca tgttcagcat gtatcgtctt aacagcaatg
    14461 atgtcatctt gatcttcagg tgtcattaat gctgaaacat taacatccgc attctgttta
    14521 gcttggtatt cctcatttac cgacgtaata cattcacgaa gccacatcgg tatgtcattt
    14581 tgatggcgcg ataataaaat taaacggtaa taatcttcct gtgcgatata atccacaagt
    14641 cgttgcatca tttgttgcaa atcagcgtca ctcataccat ctatccatac acctataaat
    14701 gtttccatca attgactact tatattaggt gattgtctcg tttcaaatgg tgtgattcga
    14761 atcattgtat tctcccgctg atacttctct tgatgactaa ttaaatcacg ttctagttgc
    14821 acactatcaa caattaaatg ggcattcttt acgatagatt gatattcctc atctgacaca
    14881 gtttcatttc tatttttaaa aaatgaataa cttaatgatt tcgctggaat atgattggct
    14941 atttgtcgat tgtgcctagc atctgaagcc acaatcacat gatcatcttc atgtatttgt
    15001 tgtgcaatca ttgcttgaaa tttttcttca attagttgag ccatattgtt atattctgtt
    15061 tgttgatagt gatgttgata tctttttgaa acagtgactc tgccattttt caaatcttca
    15121 tgaagtacac aatctccatt aatcgttaaa tattcttggt aagaagcctc tccctgatca
    15181 tcaaaataac gtatcgctga taaataacct ctgtcatcaa aaatataacg ccgttgtaac
    15241 tgatctcttt caaattcttc aaaccaaatt gaataccctt cttgactaaa ataaatattt
    15301 gtataggtct gttcactcgt cacacatttt aataaatacg gtgtgtacac aaactcaaca
    15361 tcatccggcc attttaagtg atgataatta atcgcttgtg gcgcatggtg actgaatcct
    15421 tgaatttcat caaacacaga cgaatacttt gtctcatata agtcatatcg atgtaaaaat
    15481 gttcttaaat ttggtgcatg attgagaaca atcagttgat aatctaagtc attttcaagg
    15541 tgcattccca ttaaactaat catatcgtca aattccgtct tattttgtag ttgataatac
    15601 ggcacagtcg tgtcttgcca ccatcgttgg tcatcgtacc aagctggaat aaagtatttc
    15661 ataattacct ccttaccaat actggtttaa aaatggctta tatttatcaa aatataaata
    15721 tgtacgaatt gtttctgcaa tattaatact gatgtaaact aatacaatca gttgtactga
    15781 gaaataaatt tcagtagata aatgcggtac aaacaatgtg aaataaagcg gtataccaat
    15841 aatgactgta actaatgcca atccaaacca acatacgcgt cgtgcttgat aatttaaata
    15901 acgttctgta tccttaccag gtttaactcc tgaaaaataa ttgccactct ttaagaaatc
    15961 tttggatttt tgtttagtat tgattaaaaa tctcgataaa aaataaccca ataacatttg
    16021 aatcactaaa tatactgaaa tacctactgg actatcaaat gtcagcattg gcatgtcatc
    16081 tgatatgctt ttattaaaca tagataaaat aaaatgaatg ccacttttta agaaaacaaa
    16141 agctgaaata ctcatcatta aagtaatact gcctgcaggg ttaactttcc aagataaata
    16201 agatttcata tttgttgcgg aaacgttcat taaatcgata tatggtattc tcacttctac
    16261 taattcaata aataataaga taaacaatgt gattatcaca aggatgatta acaacgcaat
    16321 cacaatatga cttgcatcta tatattccat tttttgatgc atcattgatt taataatact
    16381 aaccattaca atcggcattg gtcctgcgat gccgtagcga ctatttttgt cagctaacca
    16441 aactaataac atcgttccag taaccaaaat caatattgtt aagtaaatat tgtcttgatg
    16501 aacacgttct ttcgaaacat attcatgaat cacaaaataa ctttgaataa cacttaaaat
    16561 taatgttaag atgcgctctt tataatgctt ctcaagactt gtttgtttca tgtatttatc
    16621 catattccga taagaaatca gcatcaagat aatcattgat gttaaccacg gacctaatcc
    16681 taaagtgaaa atgtttaaag tattaacgtc tccacccata ttagaaatag ctattttaaa
    16741 aaatgactca tgttttactt gcatatcgtt ataggaaacg atggaaatgt ttgtgcctaa
    16801 tatataaata aacaagataa aacatgtgta tagcatacgt ttatatataa ttttatattc
    16861 gtattgttgt aaaagtttta acatgttgca cctcttttat atcaaaaaca ttaaaaagac
    16921 taagggttca tcactaatta ttaaaatcct atatcgattt ttctagtgat tggtgcctca
    16981 gtctttttaa ttttagccag ctataaattc aatttatgct tgagaatcat cttgatcatt
    17041 ttcatctttc tttttctttc tcttcattaa acctaaacca actaataatg tcataacgcc
    17101 acctagtaat ccattttgtt ttattgagtc acctgtatct ggcaatcttt tttcactttg
    17161 tgctggtgtg ccattatgtt tagtcacttc agatgttgca cttaatgtag actgagattc
    17221 actcgtgctc gttgttgctt cacttgataa gcgagatgtg ctcgtgctgt gagtatgatg
    17281 catactcatt gagtctgacg gatgcattga gttagattcg gatgtacttg ttgagccgga
    17341 catacttgtt gatgttgagt cagaaatgct ttgtgaacca gacatagatg tactcagtga
    17401 ttcggatgtg cttgtcgaat cggatgtgct caatgacgtt gatgtgcttg ttgacactga
    17461 ttctgagtca ctaattgatg ttgagtcgga tttgtcttgt gacattgaaa cactcgatga
    17521 attagattca ctcattgatg ttgagtcaga tacgctcgtt gaacctgaac cagacgtact
    17581 taatgattca gatatgcttg ttgaagttga accacttgtt gagtccgatg tacttgtcga
    17641 tgtcgagtct gaatctgatg tactcaatga ttctgagtca ctgatagaag ttgaatcact
    17701 tgtagattct gattctactg tactttgtga accactgata cttattgaag tagaatcact
    17761 gatactgtct gatgttgata atgatgtcga caccgatgtg ctttgtgatg acgatgtact
    17821 agcactcatt gacattgatg ttgatatcga tgtacttaag gaaccagatg cacttgtact
    17881 tgttgactgg ctttgtgaca ttgaatcact taatgatgta gatgtgcttg ttgagctcga
    17941 gtcacttaca cttgttgaac ctgatattga gtcacttaaa cttgtcgatg ttgaaactga
    18001 tacgcttccg ctcattgagt cagatgttga aagtgatgta ctcgttgaat ttgatccact
    18061 gatgctagac gaatcacttg tagacattga gtcgctttct gatgcactga tgctcataga
    18121 gtcaaattga ctattacttg ttgagcttga ctgcgaatcg ctcacacttg ttgacgttga
    18181 ttctgatcca ctcatacttt gcgagctact caatgatttt gaatcactta atgaatccga
    18241 agtgctaaga cttgtggaac cacttaaaga tattgatcca cttaatgagt cggagtcact
    18301 tgtactagta gaatcactca ttgatattga atcacttagc gaggtagact cgcttacgct
    18361 ttctgaacca cttaatgatg ttgaggtact caatgaacca gatgtacttg ttgaagtcga
    18421 accacttgtt gattttgaat cacttaatga atcagattca ctcacgcttt ctgaacttct
    18481 tagtgacgtc gatacactta atgatgacga atcgcttgtg cttactgaat cgctcatcga
    18541 ttgtgagcaa ctcaatgaac ttgactcgct tacactttct gattttctta atgacgttga
    18601 gacgctcaat gagccagaat cactgacact tgttgagcca ctcatcgatt tagagtcact
    18661 ttcagaatta gattcactta cactttctga atcatttaca gattctgaca tactttgtga
    18721 atcagatatg cttgcgctca ttacttcact agccgatgtt gatgtacttg tcgaatcact
    18781 taacgatata gacacactca tcgaaccaga tgtactcgca cttgttgagt ctgatgttga
    18841 atcactaaca ctatcagata atgacgttga atcactcata cttgttgatg tacttgtcga
    18901 aagcgacata ctttgtgaat cactagtact tgtacgcatc gaagtactag ttgaagctga
    18961 tgtactacga gagtcacttg ttgatgttga tgtacttgct gatcctgatg cacttgtact
    19021 tcttgatgtg ctttgtgaat cgcttaatga tgttgatgtt gaatcggatt cacttgtact
    19081 ttctgatgtt gagccagact ctgatgtact taccgatgta gataaacttg caatggtcga
    19141 catgcggttt gaagttgatg tacttagcga atcacttaat gatgctgatg tgctttgtga
    19201 atcggattca ctgctttgca ttgacgcaga tgttgaatca cttaaagatg ttgacgacaa
    19261 atcggattca cttgcgcttg ctgagttact gagcgatgtt gacgtacttg ctgatcctga
    19321 gttacttgta cttgttgagt cactgagtga tgttgacgca ctttctgatg ttgactcact
    19381 tgcacttgtt gagatacttt gtgaatcaga ttcactctct gaaccactta atgacgctga
    19441 tgtgcttacc gaacctgatt gactcgtact tttagatata cttgttgaat cgcttagtga
    19501 cgttgatgtc gatgtggatt cactttggct tgttgactga cttgtatatg atgatgtgct
    19561 ttgtgaatcg gattcgctcg tgcttgtact tgttgagttt gaggcacttt ggcttgctga
    19621 gtttgagtct actccgcttt gattcattga ggcacttagt gacaatgatg tactcgttga
    19681 gtcagacaaa cttgtactcg ttgacgtact tgtacttcct gatgttgatt gagacatact
    19741 tatgctcatt gatgttgaat cggatttact ttcacttgat gatgttgagt cggattcact
    19801 ttcacttgta gaaccactta atgatgtgga tgtactaatg gaatcagatt tacttgtact
    19861 gattgaatca cttgtcgaca ttgatgtact taatgaatca gacttactat cacttgtgga
    19921 atcacttaat gatgttgaca aacttgtaga gtcagacaaa cttgtactcg ttgacatact
    19981 cagtgatgtt gaaacactct cgctcttaaa cgttgacgtt gattcactga tacttgtcga
    20041 tgttgaaatg gacgtactac cacttgttga attacttaat gatgttgatg tgctaccaga
    20101 ttctgatgta ctgtctgata atgacgtact ctcacttgtc gaactactca ctgactctga
    20161 tgttgattca gacgtacttt cacttaatga ttcacttaaa aaggcagatg cactttgtga
    20221 ttctgaatcg ctagtactat ttgattcact taatgatata aacgtgctct ctgaagcaga
    20281 tattgcttca cttatagagt cgctcgttga cgttgattca cttattgaat cagactctga
    20341 tgtacttaag cttgtggaat cactcataga tgttgatgta cgttctgaat tacttgtact
    20401 taacgatgtc gaagtactta ccgaaccaga tgtgctcgta aaagcacttt gtgatattga
    20461 ttcacttgat gcagttgatg ctgatttgct atcactcgtt gaatcactaa acgacgttga
    20521 tatgctcatt gaatcggatt gacttgcact cattgaacca gacgtacttt gtgattccga
    20581 agtacgtact gaagcacttg tcgacgttga tgtacttgtt gaacctgatt gactagtact
    20641 ttgtgataat gacttactat cagaatcaga tgtactttgt gaatcactta atgattctga
    20701 tgtactacct gactgagacg tgctcattga actacttacg gacattgatt tactgtctga
    20761 tgcagataat gacccacttg tactgataga gtcacttact atctctgaag tactcatcga
    20821 gtctgatgta cttgttgaga cactttgtga tgctgctatg cttagtgatc cagaaacaga
    20881 accacttgtg ctcgtcgaat cgctcaatga ttctgatgta ctcatcgatt ttgaatcact
    20941 tgtacttaat gatattgatg tactttgtga atctgatttg cttgttgacg cactttgaga
    21001 gttggctatg ctatttgaaa tactgataga gtccgaggtg ctagctgact cgctcaatga
    21061 tgttgatgta ctaattgcat tcgatgtact gtcacttaat gatgctgatg tactagacga
    21121 ccctgatata ctcgttgata agctttgtga cttagacaag cttcctgatg tactcatact
    21181 taatgagtca ctgagtgatg ttgatgtacg caatgaatca gatgtacttg ttgatagact
    21241 ttcggatttt tcagtactgc tagagtttga aatagaatcg cttaatgatg ttgatttact
    21301 agctgaatcc gacatgcttg atgatacact ttgtgaattc actaaacttg tgcttgttga
    21361 gcttgataca ctattacttt cagatgtgct taatgactta gatgcactca cagaatcaga
    21421 taggcttaca cttgtcgatt tcgaggtact agctgatgta gataccacaa tcgatcctga
    21481 tgtactcgtt gatgcacttt gtgagtcagc tttacttgtt gacacacttt gagattgttg
    21541 tgtacttcct gatgttgata cggaatcact catgctattt cttgttactt catatttaaa
    21601 agttgtcgtc gttttgttac cgctcgcatc tgtagaaacg attgatatag tacttgtacc
    21661 aatgtttgtt ggtgtaccac taatagtatt atttgtacta tcaaatgtta gtccggatgg
    21721 caatccagtc actgtattcg tcaccgcatt tccactgtta tcttgcgtag caattttaat
    21781 cggggatatt ggtgaataca cttctgatga ttgatctcct attggtgtca ctgttggtgc
    21841 tgtcgtatcc acaacattta ttgtaaaagt tgtcgtcgat ttgttatttg cttggtcagt
    21901 agacacaact gtcactgttg attgaccaat ttttgttggt gtcccaatga ttgaattcgt
    21961 tgcactatcg taacttaatc cgcttggtaa tcctgtaact gtatttgtca cagtcccagt
    22021 accattatcc gttgtagtca atacaatagg attcattgtt ttacccactt ctatggtttg
    22081 attgcctaca gttacagttg gtgcttttac atcagtaaaa taatatgtca ctgattgtcc
    22141 agcattcgtc atttttacag ttttatttgt atcattataa gttgacgcat atgaactatc
    22201 gacggacgtg tagttatatc ctttagcagt caatgcagat tgctgattat cgattgtcac
    22261 gacttgatca acatttcctg aatatgtttt tggtggaata atatctttac ctgttgttac
    22321 atcaacgtat ctcacttgtg taacagcaga ctctgtatat tcgaatgttc caaattgtac
    22381 ttgttgtaaa tttgtcgcgc cacctgttga ggctgtcatt gataatgaaa agttggtcgt
    22441 accacttttc gcaatccaat ctgaaatatt acgtgtccat gtttgacctg catatttgac
    22501 agtcataacc tttgtatcac cattatagtt aatatcaaaa tcttggaacg tgttatttgt
    22561 aggttgaaca tttaacttcg cagcattatc agctgttgaa cttgatgtat acgttgtcgc
    22621 aacaccataa ctatctgttg ttacaaatgc accaaacgca cctccaccag ctacattaga
    22681 tgggtcagca ttcgcctttg cagctgaatt tggtttagat gtattgtgat acgtatccaa
    22741 tttgaagcca aatgcgttac ttaagccacc aatacctact gcggcaccgt ttaaccctgt
    22801 ttcacctaat acacctggtg aaaaggcaaa accgatacca tctccaccat ttccatgccc
    22861 ttcatatttg ttacctaaat ttacttttcc agaaaaatga aaactcttat tagagtcaat
    22921 acgtgttcct aatgtaatag cacctttttg gctgtatgca tcctgtgtta acgtcacaat
    22981 accggtactt tgatcatagg tagcattacc tgacgttgtc atatattgtt ttaagttatc
    23041 tttattaact gtaattgtat tagcagttac tgcggttgtc gtcgctgctg acgcaaatgt
    23101 tgacatagct aagcgactga aagttcgaag ttttactggt gcggtgctag ttgacgttgt
    23161 gctagttttg tttaagttga ccgaagatgg cgttgtgctt tgtgaagtgt tatttgatgc
    23221 agtactttga tttgttgatg tattaattgg ttgttctgta cttgaagttg aagctacaga
    23281 tttagtatca gaacttgatg tagtattctt tgaggatgtt gattctgatg tagatgtcaa
    23341 tttctcttgt tgattgcttg tactattagt tgtcgaagtg accttttcag acttttcact
    23401 tgagactgtg tcactatttg atgtttgtac cgaactacta tttttcgtta cacttgtgga
    23461 atcggctgtt gatgttgatg cttcgattgt cgttgagttt tgattaccta ctgtttcact
    23521 ttgtgtgttt aattcagaag ttaatggtgc atcagaagcc gcaaaagctt gctggtcatg
    23581 caacatattt accgtgaatg caccaccaat aaccgccgta gttttcagtc cgtatcccgt
    23641 cattttttta ctaatgcttt gattatcttg actcactaaa ctatgactaa taaatggtag
    23701 ccccataatt ttgaacattt ctatttcttt aattccggat tttacccaat tttttccaga
    23761 tttataaagt cttactcttg ttttttcgtt tgctaagctg tcatgaaatg ctttctgtct
    23821 tttactcatg taataactcc ttgtattatc tttacattca ttagattata atatatgcca
    23881 ctattcaatt taatacaact cttttttgat acaaaaatac
//
LOCUS       DALAGD010000040        23730 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.040, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000040 DALAGD010000000
VERSION     DALAGD010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 23730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23730
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.040"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            95..208
                     /locus_tag="O2701_001268"
     CDS             95..208
                     /locus_tag="O2701_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293171.1"
                     /translation="MKKVYIKLKYIVRYHLAFVFISSFSLNFSKQKYVTVN"
     gene            281..1270
                     /gene="ccpA"
                     /locus_tag="O2701_001269"
     CDS             281..1270
                     /gene="ccpA"
                     /locus_tag="O2701_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HCZ4293172.1"
                     /translation="MTVTIYDVAREARVSMATVSRVVNGNQNVKAETKNKVNEVIKRL
                     NYRPNAVARGLASKKTTTVGVIIPDISNIYYSQLARGLEDIATMYKYHSIISNSDNDP
                     EKEKEIFNNLLSKQVDGIIFLGGTITEEMKELINQSSVPVVVSGTNGKDAHIASVNID
                     FTEAAKEITGELIEKGAKSFALVGGEHSKKAQEDVLEGLTEVLNKNGLQLGDTLNCSG
                     AESYKEGVKAFAKMKGNLPDAILCISDEEAIGIMHSAMDAGIKVPEELQIISFNNTRL
                     VEMVRPQLSSVIQPLYDIGAVGMRLLTKYMNDEKIEEPNVVLPHRIEYRGTTK"
     gene            complement(1368..2537)
                     /locus_tag="O2701_001270"
     CDS             complement(1368..2537)
                     /locus_tag="O2701_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HCZ4293173.1"
                     /translation="MQQHSSKTAYVYSDKLLQYRFHDQHPFNQMRLKLTTELLLNANL
                     LSPEQIVQPRIATDDELMLIHKYDYVEAIKHASHGIISEDEAKKYGLNDEENGQFKHM
                     HRHSATIVGGALTLADLIMSGKVLNGCHLGGGLHHAQPGRASGFCIYNDIAITAQYLA
                     KEYNQRVLIIDTDAHHGDGTQWSFYADNHVTTYSIHETGKFLFPGSGHYTERGEDIGY
                     GHTVNVPLEPYTEDASFLECFKLTVEPVVKSFKPDIILSVNGVDIHYRDPLTHLNCTL
                     HSLYEIPYFVKYLADSYTNGKVIMFGGGGYNIWRVVPRAWSHVFLSLIDQPIQSGYLP
                     LEWINKWKHYSSELLPKRWEDRLNDYTYVPRTKEISEKNKKLALHIASWYESTRQ"
     gene            complement(2562..3194)
                     /locus_tag="O2701_001271"
     CDS             complement(2562..3194)
                     /locus_tag="O2701_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCZ4293174.1"
                     /translation="MNHLKTYQSEDYYIHDKQFVIEGPLTHEDLKALTFDAHLTAFRD
                     AEDQYKALLEITTLPEGRIYVARQDQLIVGYVTFHYPDEIERWSTGKLPYLIELGAIE
                     VSINFRQLQLAEKLIQLSLSTPEFENYIVITTEYYWHWDLKNSKLDVFDYKKLMQRLM
                     ATGGLEIFATDDPEITSHPANCLMARIGKNITLEQQQAFDDIRYMNRFFF"
     gene            3363..5069
                     /gene="acsA"
                     /locus_tag="O2701_001272"
     CDS             3363..5069
                     /gene="acsA"
                     /locus_tag="O2701_001272"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCZ4293175.1"
                     /translation="MKVEVYKGAQGKHNLKDYEETYNTFDWKDVEQAFSWSETGKMNM
                     AYECIDRHVDQGLGDKIALNYKDEHRKESYTYKDMQRLSNKAANVLSEHAEVDKGDRV
                     FIFMSRTPELYFALLGVLKIGAIVGPLFEAFMEKAVADRLENSEAKVLITNKALLPRV
                     PVDKLPNLKKIVVVDEDVEDNYIDFISLMETASDEFDIEWLKSDDGLILHYTSGSTGQ
                     PKGVLHVQQAMLVHYISGKYVLDLQEDDVYWCTADPGWVTGTSYGIFAPWLNGATNCI
                     AGGRFSPEQWYSMIEDFKVTIWYTAPTALRMLMSAGDDIVEKYDLSSLRSILSVGEPL
                     NPEVIKWAKKVYGLTVLDTWWMTETGGHMIVNYPTMDVKLGSMGKPLPGIQAAIIDDA
                     GNELPPNRMGNLAIKKGWPSMMYRIWKNPEKYKSYFIGDWYVSGDSAYKDEDGYFWFQ
                     GRVDDVIMTAGERVGPFEVESKLVEHEAVAEAGIIGKPDPVRGEIIKAFVALRKGYEP
                     TDELKEEIRIFVKEGLSAHAAPREIEFKDKLPKTRSGKIMRRVLKAWELNLDAGDLST
                     ME"
     gene            5509..7176
                     /locus_tag="O2701_001273"
     CDS             5509..7176
                     /locus_tag="O2701_001273"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HCZ4293176.1"
                     /translation="MTHLSDLDIANQSTLQPIKDIAASVGISEDALEPYGHYKAKIDI
                     NKITPRENKGKVVLVTAMSPTPAGEGKSTVTVGLADAFHELNKNVMVALREPALGPTF
                     GIKGGATGGGYAQVLPMEDINLHFNGDFHAITTANNALSAFIDNHIHQGNELGIDQRR
                     IEWKRVLDMNDRALRHVNVGLGGPTNGVPREDGFNITVASEIMAILCLSRSIKDLKDK
                     ISRITIGYTRDRKPVTVADLKVEGALAMILKDAIKPNLVQSIEGTPALVHGGPFANIA
                     HGCNSILATETARDLADIVVTEAGFGSDLGAEKFMDIKVREAGFDPAAVVVVATIRAL
                     KMHGGVAKDNLKEENVEAVKAGIVNLERHVNNIKKFGVEPVVAINAFIHDTDAEVEYV
                     KSWAKENNVRIALTEVWKKGGKGGVDLANEVLEVIDQPNSFKPLYELELPLEQKIEKI
                     VTEIYGGSKVTFSSKAQKQLKQFKENGWDNYPVCMAKTQYSFSDDQTLLGAPSGFEIT
                     IRELEAKTGAGFIVALTGAIMTMPGLPKKPAALNMDVTDDGHAIGLF"
     gene            7472..10159
                     /gene="harA"
                     /locus_tag="O2701_001274"
     CDS             7472..10159
                     /gene="harA"
                     /locus_tag="O2701_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haptoglobin-binding heme uptake protein HarA"
                     /protein_id="HCZ4293177.1"
                     /translation="MNKHHPKLRSFYSIRKSTLGVASVIVSTLFLITSQHQAQAAENT
                     NTSDKISENQNNNATTTQPPKDTNQTQPATQPANTAKNYPAADESLKDAIKDPALENK
                     EHDIGPREQVNFQLLDKNNETQYYHFFSIKDPADVYYTKKKAEVELDINTASTWKKFE
                     VYENNQKLPVRLVSYSPVPEDHAYIRFPVSDGTQELKIVSSTQIDDGEETNYDYTKLV
                     FAKPIYNDPSLVKSDTNDAVVTNDQSSSVASNQTNTNTSNQNTSTINNANNQPQATTN
                     MSQPAQPKSSTNADQASSQPAHETNSNGNTNDKTNESSNQSDVNQQYPPADESLQDAI
                     KNPAIIDKEHTADNWRPIDFQMKNDKGERQFYHYASTVEPATVIFTKTGPIIELGLKT
                     ASTWKKFEVYEGDKKLPVELVSYDSDKDYAYIRFPVSNGTREVKIVSSIEYGENIHED
                     YDYTLMVFAQPITNNPDDYVDEETYNLQKLLAPYHKAKTLERQVYELEKLQEKLPEKY
                     KAEYKKKLDQTRVELADQVKSAVTEFENVTPTNDQLTDLQEAHFVVFESEENSESVMD
                     GFVEHPFYTATLNGQKYVVMKTKDDSYWKDLIVEGKRVTTVSKDPKNNSRTLIFPYIP
                     DKAVYNAIVKVVVANIGYEGQYHVRIINQDINTKDDDTSQNNTSEPLNVQTGQEGKVA
                     DTDVAENSSTATNPKDASDKADVIEPESDVVKDADNNIDKDVQHDVDHLSDMSDNNHF
                     DKYDLKEMDTQIAKDTDRNVDKDADNSVGMSSNVDTDKDSNKNKDKVIQLNHIADKNN
                     HTGKAAKLDVVKQNYNNTDKVTDKKTTEHLPSDIHKTVDKTVKTKEKAGTPSKENKLS
                     QSKMLPKTGETTSSQSWWGLYALLGMLALFIPKFRKESK"
     gene            10290..10382
                     /locus_tag="O2701_001275"
     CDS             10290..10382
                     /locus_tag="O2701_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293178.1"
                     /translation="MKIILLLFLIFGFIVVVTLKSEHQLTLFSI"
     gene            complement(10362..11267)
                     /locus_tag="O2701_001276"
     CDS             complement(10362..11267)
                     /locus_tag="O2701_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCZ4293179.1"
                     /translation="MTNQDNNHQLNHRIYHFEKIYKAIKHVIVFIFMIFIAIVAIAVI
                     AMSLYFHHLTKTSDSLSDDALIKKVRQIPGDELLDHNNKNLLYEYNHSQNSLIIGPKT
                     SSPNVIKALTSSEDTLFYKHDGILPKAILRAMIQDIFNTDQSSGGSTITQQLVKNQVL
                     TNEKTYSRKANELRLAIRLEHLLSKDEIIYTYLNIVPFGRDYNGANISGIASASYSLF
                     GIPPKDLSIAQSAYLIGLLQSPYGYTPYEKDGTLKSDKDLKYSIQRQHYVLKRMLIED
                     QITEKEYNDALKYDIKSHLLNRKKR"
     gene            11649..12911
                     /locus_tag="O2701_001277"
     CDS             11649..12911
                     /locus_tag="O2701_001277"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCZ4293180.1"
                     /translation="MTNVLIEDLKWRGLIYQQTDEQGIEDLLNKEQVTLYCGADPTAD
                     SLHIGHLLPFLTLRRFQEHGHRPIVLIGGGTGMIGDPSGKSEERVLQTEEQVDKNIEG
                     ISKQMHNIFEFGTDHGAVLVNNRDWLGQISLISFLRDYGKHVGVNYMLGKDSIQSRLE
                     HGISYTEFTYTILQAIDFGHLNRELNCKIQVGGSDQWGNITSGIELMRRMYGQTDAYG
                     LTIPLVTKSDGKKFGKSESGAVWLDAEKTSPYEFYQFWINQSDEDVIKFLKYFTFLGK
                     EEIDRLEQSKNEAPHLREAQKTLAEEVTKFIHGEDALNDAIRISQALFSGDLKSLSAK
                     ELKDGFKDVPQVTLSNDTTNIVEVLIETGISPSKRQAREDVNNGAIYINGERQQDVNY
                     ALAPEDKIDGEFTIIRRGKKKYFMVNYQ"
     gene            complement(13005..14279)
                     /locus_tag="O2701_001278"
     CDS             complement(13005..14279)
                     /locus_tag="O2701_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCZ4293181.1"
                     /translation="MSDFNHTDHSTTNHSQTPRYRRPKFPWFKTVIVALIAGIIGALL
                     VLGIGKVLNSTILNKDGSTVQTTNNKGGNQLDGQSKKFGTVHEMIKSVSPTIVGVINM
                     QKASSVDDLLKGKSSKPSEAGVGSGVIYQINNNSAYIVTNNHVIDGANEIRVQLHNKK
                     QVKAKLVGKDAVTDIAVLKIENTKGIKAIQFANSSKVQTGDSVFAMGNPLGLQFANSV
                     TSGIISASERTIDAETTGGNTKVSVLQTDAAINPGNSGGALVDINGNLVGINSMKIAA
                     TQVEGIGFAIPSNEVKVTIEQLVKHGKIDRPSIGIGLINLKDIPEEEREQLHTDREDG
                     IYVAKADSDIDLKKGDIITEIDGKKIKDDVDLRSYLYENKKPGESVTVTVIRDGKTKE
                     VKVKLKQQKEQPKRQSRSERQSPGQGDRDFFR"
     gene            14451..15068
                     /locus_tag="O2701_001279"
     CDS             14451..15068
                     /locus_tag="O2701_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HCZ4293182.1"
                     /translation="MYSVISKILNFILVKMSKSLYVIGKDNIPKDSKYVVTCTHESYN
                     EVIMLGMALYPNQIHYMAKKELFKNKWIGKFLTSLNAFPVDRENPGPSTLKRPINLLK
                     DNKTVGIFPTGSRTSQEGAPLKRGASTIAMLSKSPILPVAYVGPTKIHGLLTGQAYIN
                     IGKPIDINDLPKDLKRNERIDYITKEIETRTAKLQQELHEIVKSL"
     gene            15196..16662
                     /locus_tag="O2701_001280"
     CDS             15196..16662
                     /locus_tag="O2701_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIBC"
                     /protein_id="HCZ4293183.1"
                     /translation="MYKFFQNLGRSLMLPVAILPAAAIIAGIGNTLNALHATPKIAMF
                     FTTVGTTILEQLGILFAIGVAIGMAKKNDGVVALAATLGYFLVTVVLSPMKLAPLLGM
                     KASEINSAFEKMNNGNVFVGIVIGLIAAYAYNKFSETELPLALSFFSGKRLVPIMTAF
                     YCTFLVVILLFLWPLLYSWIVKFGESIVGLGSFGAFIYGVANRLLIPTGLHHALNSVF
                     WFDTIGINDIGKFQSGKDAIKGITGRYQAGFFPIMMFGIPAAALAMYHTAKTTQKKQV
                     YGWFLASSVAAFFVGVTEPIEFAFMFVAPILYVVHALLTGLSLFIAATFHWTAGFSFS
                     AGLIDYVLSLINPVSNHPLMLLVQGVVFFILYYVIFRVVIQVFNLNTIGRGENELVDP
                     TVVKDNIAPGENDIKQSKYHQHAIQILEGLGGQENIVNLTNCATRLRLELKDTSIIDK
                     QKIKNAGAVGVTVNGKHSTQVIVGTHVQQVADEIEKHL"
     gene            16769..17899
                     /locus_tag="O2701_001281"
     CDS             16769..17899
                     /locus_tag="O2701_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCZ4293184.1"
                     /translation="MKKILFDVDGVFLSEERCFDVSALTVYELLMDKCYLGLHSHIDW
                     ETLTDNDIQDIRNRIFQKDKILNKLKSLGLNSNWDMLFIVFSIHLIDILKKLSHDEIE
                     AFMYQDEPVELKLQNISTNLADCFNLNEQLPLQFLDNVKVGKNNIYAALEEFATTELH
                     VSDATLFSLKGALWTLAQEVYQEWYLGSKLYEDVEKKIARTTFKTGYIYQEIILRPVD
                     EVKVLLNDLKGAGFELGIATGRPYTETVVPFENLGLLPYFEADFIATASDVLEAENMY
                     PQARPLGKPNPFSYIAALYGNNRDKYESYINKQDNIVNKDDVFIVGDSLADLLSAQKI
                     GATFIGTLTGLKGKDAAGELEAHHADYVINHLGELRGVLDNL"
     gene            complement(17860..17955)
                     /locus_tag="O2701_001282"
     CDS             complement(17860..17955)
                     /locus_tag="O2701_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293185.1"
                     /translation="MKPLVQSFTTSYAVKQQSNYKLSSTPLSSPK"
     gene            complement(18049..19653)
                     /locus_tag="O2701_001283"
     CDS             complement(18049..19653)
                     /locus_tag="O2701_001283"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCZ4293186.1"
                     /translation="MKQFNVLVADPISKDGIKALLDHEQFNVDIQTGLSEEALIKIIP
                     SYHALIVRSQTTVTENIINAADSLKVIARAGVGVDNINIDAATLKGILVINAPDGNTI
                     SATEHTLAMLLSMARNIPQAHQSLTNKEWNRNAFKGTELYHKTLGVIGAGRIGLGVAK
                     RAQSFGMKILAFDPYLTDEKAKSLSITKATVDEIAQHSDFVTLHTPLTPKTKGLINAV
                     FFAKAKPSLQIINVARGGIIDEKALIKALDEGQISRAAIDVFEHEPATDSPLVAHDKI
                     IVTPHLGASTVEAQEKVAISVSNEIIEILIDGTVTHAVNAPKMDLSNIDDTVKSFINL
                     SQTVGELAIQLMYNAPSSIKITYGGDLASIDSSLLTRTIITHILKDDLGPEVNIINAL
                     MLLNQQQVTLNIENNKAETGFSNYLEVELSNDSDSVKVGASVFTGFGPRIVRINNFSV
                     DLKPNQYQIVSYHNDTPGMVGKTGALLGKYNINIASMTLGRTEAGGDALMILSVDQPV
                     SNNIIDELKQVGEYNQIFTTELTVQS"
     gene            complement(19640..20800)
                     /locus_tag="O2701_001284"
     CDS             complement(19640..20800)
                     /locus_tag="O2701_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HCZ4293187.1"
                     /translation="MYYHQPLLLTPGPTPVPDAIMREIQAPMVGHRSKDFEDIAQQAF
                     QGLKPIFGSQNDVLILTSSGTSVLEASMLNIVNPEDHFVVIVSGAFGNRFKQIAQTYY
                     KNVHIYDVTWGEAVDVKDFINFLSTLNVEVKAVFSQYCETSTTVLHPIHELGNAINQF
                     NSNIYFVVDGVSCIGAVDVDINKDKIDVLVSGSQKAIMLPPGLAFVAYSHRAKEHFKE
                     VTTPKFYLDLNKYISSQADNSTPFTPNVSLFRGVNAYVETVKAEGFNHVIARHYAIRN
                     ALRSALKALDLTLLVNDKDASPTVTAFKPNTNDEVKIIKDELKNRFKITIAGGQGHLK
                     GQILRIGHMGKISPFDILSVVSALEIILTEHRKVNYIGKGISKYMEVIHEAI"
     gene            complement(20914..21360)
                     /locus_tag="O2701_001285"
     CDS             complement(20914..21360)
                     /locus_tag="O2701_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCZ4293188.1"
                     /translation="MHQHDFKVQTSWQGGRNNVGNVQGDILSENISIPASLGGVGIGT
                     NPDEMLVSAASSCYIISLAATLERAKFTDISIEQQSIGTACLNNGKFSMSKIVHHPQI
                     QIPSDQIAQLEKRLPKLITIADNNCMISNAVRNNVDIKIYPIIQAK"
     gene            21440..22183
                     /locus_tag="O2701_001286"
     CDS             21440..22183
                     /locus_tag="O2701_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCZ4293189.1"
                     /translation="MTLNKLKDELQIVSHRGLPSDFPENTMVGYREVMGLNVAMLEID
                     VHLTKDQHFVVIHDETIDRTSDGKGRIADYTLSQLKSFDFGSYKDVAFKGERIPTLDE
                     VLSLCLKYDKKLLIELKSPNLYPEIECKLLAFLEEKKVDATQVVIQSFDIECIEKLNT
                     LGSIYELGVLCSKRNYWYKKPNFSRIAQIASYVNPNYALVTRKFVDKAHHHQLQVMPY
                     TVNKLKTGEKLRQMGVDGLITDNPKLFIK"
     gene            complement(22399..23001)
                     /gene="rpsD"
                     /locus_tag="O2701_001287"
     CDS             complement(22399..23001)
                     /gene="rpsD"
                     /locus_tag="O2701_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019165370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCZ4293190.1"
                     /translation="MARFRGSNWKKSRRLGISLSGTGKELEKRPYAPGQHGPNQRKKL
                     SEYGLQLREKQKLRYLYGMTERQFRNTFDIAGKKFGVHGENFMILLASRLDAVVYSLG
                     LARTRRQARQLVNHGHILVDGKRVDIPSYSVKPGQTISVREKSQKLNIIVESVEINNF
                     VPEYLNFDADSLTGTFVRLPERSELPAEINEQLIVEYYSR"
     gene            complement(23245..23709)
                     /locus_tag="O2701_001288"
     CDS             complement(23245..23709)
                     /locus_tag="O2701_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HCZ4293191.1"
                     /translation="MTTINPTNYTLLKKQAASLIEDEHHMIAILSNMSALLNDNLDQI
                     NWVGFYLLEQNELILGPFQGHPACVHIPIGKGVCGTAVSERRTQIVADVHQFEGHIAC
                     DANSKSEIVVPIFKDDKIIGVLDIDAPITDRFDDNDKEHLEAIVKIIEKQLA"
BASE COUNT         8126 a         3562 c         4303 g         7739 t
ORIGIN      
        1 taatagattc acacatttgt tgctgaaatg tgaacatttt tcaacaaatg caattgatat
       61 ttgaaaaggc tttctcaaaa cattacaatt aaaaatgaaa aaagtttata taaaattaaa
      121 atatatcgtt cgttatcatt tagcgtttgt ttttatttca agcttttcgc taaatttttc
      181 caaacaaaaa tatgttactg taaattaaaa tatggtaaac tatgaaaatg aaatgaaaac
      241 atgttattat aatgaataaa acgtttacaa ggaggaaatt atgacagtta ctatatatga
      301 tgtagcaaga gaagcgcgtg tctctatggc cacagtgtcg cgtgttgtta atgggaacca
      361 aaatgttaaa gcagaaacta aaaataaagt taacgaagtc attaagcgtt tgaattatcg
      421 tccaaatgct gttgctagag gtttagctag taaaaagaca acaacagtag gtgtgatcat
      481 tccagatata tctaatatct attattcaca acttgctcgt ggacttgaag atattgcaac
      541 aatgtataaa tatcactcaa ttatttcaaa ttcagataac gatcctgaaa aggaaaaaga
      601 aatttttaat aacttattaa gtaaacaggt tgatggtatt attttccttg gtggtacaat
      661 tactgaagaa atgaaagaat tgataaatca atcatctgta cctgtagtag tatcaggaac
      721 aaatggtaag gatgcacata tagcatcagt taatattgat tttactgaag ctgcgaaaga
      781 aattacggga gaattaattg aaaaaggcgc taaatcattt gctttagtag gtggagaaca
      841 ttctaaaaaa gctcaagaag atgttttaga aggtttaact gaagtgttaa ataaaaatgg
      901 ccttcaatta ggtgatacat tgaattgttc tggtgctgaa agttataaag aaggcgtaaa
      961 agcttttgcc aaaatgaaag gcaatttgcc agatgccatt ttatgtatca gcgacgaaga
     1021 agcaattggt attatgcata gtgcaatgga tgctggtatt aaagttccag aggaattaca
     1081 aattattagt ttcaataata cacgattagt tgagatggtt agaccacaac tttctagtgt
     1141 tattcaacca ttatatgata tcggtgcagt agggatgcgc ttattaacaa aatatatgaa
     1201 cgatgaaaag atagaagaac caaatgtagt tttacctcac agaattgaat accgaggaac
     1261 tacaaaataa attcacaaaa ttaggcattc atctaacgac ccaaattata tgggtgttgg
     1321 aagaatgcct tttatttatc ttttaaaatc gttgcagatt aggttactta ttgacgagta
     1381 gattcgtacc aactcgctat atgtaaagct aattttttat ttttttcact aatttctttt
     1441 gtgcggggga cataggtata atcatttaaa cgatcttccc atcttttagg taataattca
     1501 gatgaataat gtttccattt attaatccat tctaacggta aataaccact ttgaattggt
     1561 tgatcaatta aacttaagaa tacatgactc catgcacgtg gtacgactct ccaaatattg
     1621 tagcctccgc caccaaacat aattaccttt ccattcgtat aagaatcagc taaatatttt
     1681 acaaaatatg gaatttcata taatgaatgt aacgtacaat ttagatgagt tagtggatca
     1741 cgataatgta tatcgacacc atttacgctt agaataatat caggtttaaa actctttacg
     1801 acaggctcaa ctgttaattt aaaacactcc aaaaatgatg catcttctgt atacggttca
     1861 agtgggacat ttacagtgtg tccatagccg atatcttcac cgcgctcagt atagtgacca
     1921 gagcctggga aaagaaattt tccggtttca tggatagaat aagtagtaac atggttatcg
     1981 gcatagaaac tccattgtgt accatctcca tgatgtgcat cggtatctat gattaaaacg
     2041 cgttgattgt attctttagc taagtattgt gcggtaattg caatatcatt gtatatacaa
     2101 aaaccacttg ctcgaccagg ttgagcgtga tgcaaaccac cacctaagtg acaaccattt
     2161 aatactttgc ctgacataat aagatctgct aaagttaaag cgcctccaac aattgtggca
     2221 ctatggcggt gcatatgctt aaattgacca ttctcttcat catttaatcc atatttctta
     2281 gcctcatctt cactgataat gccatgtgaa gcatgcttaa tagcttcgac gtaatcatat
     2341 ttatgaatta acattaattc gtcatctgtt gcaattctag gttgtactat ttgttctgga
     2401 gacaataaat ttgcattcaa aagtagctct gttgttaatt ttaaacgcat ttgattgaag
     2461 ggatgttggt catgaaatcg atattgtaat aacttatctg aataaacata tgcagttttt
     2521 gatgaatgtt gttgcatata atccctccga tattccaaaa attaaaagaa aaaccgattc
     2581 atataacgaa tatcatcaaa cgcttgttgc tgttctaatg taatgttttt gccaattctt
     2641 gccattaaac aattagctgg atgacttgtt atttctggat catctgtagc gaatatttca
     2701 agtccaccag ttgccattaa ccgctgcatt aattttttat agtcaaatac atctaacttt
     2761 gaatttttta aatcccaatg ccagtaatat tctgtagtta taacgatata attctcgaat
     2821 tctggtgtag aaaggctaag ttgtatcagc ttttctgcaa gttgtaattg cctaaaattg
     2881 atgctgactt caattgcccc caattcgatt aaatatggaa gcttacctgt agaccagcgc
     2941 tcaatttcat caggatagtg gaaagtgaca taacccacaa tgagttgatc ttggcgagca
     3001 acataaattc taccttctgg taatgttgta atttctaaca aagctttata ctgatcttca
     3061 gcatctctaa atgcggttaa atgcgcatcg aaagtaagcg ctttcaaatc ttcgtgtgtt
     3121 aaaggacctt caataacaaa ttgcttgtca tgaatgtaat aatcttcgga ttgatacgtc
     3181 tttaaatgat tcatattttc aactcctcaa tcgacgtcgt gattgtatta aattcattat
     3241 atagaaaatt tacaataatt aatactagaa aaaggataaa agtaaaaatt ttgaataatt
     3301 agaaatgtta tgtataatat tgagaaagaa agcgttttca cataacaaag ggggagtttc
     3361 aaatgaaagt cgaagtttat aaaggagcgc aaggtaaaca taaccttaaa gattatgaag
     3421 aaacatataa tacttttgat tggaaagacg tagaacaagc attttcttgg agtgaaactg
     3481 gaaaaatgaa catggcatat gaatgcatag atcgccatgt agatcaagga ttaggggata
     3541 aaatagcgtt aaattacaaa gatgagcaca gaaaagaatc gtatacttat aaagatatgc
     3601 aacggttatc taataaagca gcgaatgttt tgtctgaaca tgcagaagtt gacaaaggtg
     3661 acagagtatt tatatttatg tcgcgtacac ctgaactata ttttgcgttg ttaggtgttt
     3721 taaaaattgg tgcaattgtt gggccgttat ttgaagcatt tatggaaaag gcagttgcgg
     3781 atagattaga gaacagtgaa gctaaagtgt taattactaa taaggcattg ttacctcgag
     3841 tacctgtaga taaattacca aacttgaaaa aaattgttgt cgtagatgag gatgtagaag
     3901 acaattacat agacttcatt agtttgatgg aaactgctag cgatgaattt gacattgaat
     3961 ggttaaagtc ggatgatggt ttgattttac attatacatc aggttctact gggcaaccta
     4021 aaggtgtatt gcatgttcaa caagcaatgt tagtgcacta tatttctgga aaatatgtat
     4081 tagatttaca agaagatgat gtttattggt gtacagcaga tccaggttgg gttacaggaa
     4141 catcttatgg tatttttgca ccatggttaa atggcgctac aaattgtata gctggtggtc
     4201 gcttttcgcc agaacagtgg tatagtatga ttgaagattt taaagtgacg atttggtata
     4261 cggcaccaac agctttaaga atgttaatga gtgctggtga cgatattgtt gagaaatatg
     4321 acttgtcatc gttacgttcg attctatcag taggtgagcc tttaaatcct gaagttataa
     4381 aatgggcgaa aaaagtatac ggtttaacgg tgttagatac ttggtggatg acagaaacag
     4441 gtggacatat gattgttaac tatccaacga tggacgtcaa gcttggctca atgggcaaac
     4501 cattacctgg tattcaagct gcaattatcg atgatgcagg gaatgaatta ccaccaaatc
     4561 gaatgggcaa ccttgctata aaaaaaggct ggccatcaat gatgtatcgt atctggaaga
     4621 atccagaaaa atataaatca tattttattg gagactggta tgtatctggt gattcggcat
     4681 ataaagatga agatggttac ttctggttcc aaggacgtgt tgatgatgta attatgacag
     4741 ctggtgaacg agttggacca tttgaggttg agtctaaatt ggttgaacac gaagcagttg
     4801 ccgaagcagg aattattggt aaacctgatc cggttcgcgg tgaaataatt aaggcgtttg
     4861 ttgcactgag aaaaggatat gaaccaacag acgaattaaa agaagaaatt cgtatatttg
     4921 ttaaagaagg tttgtcggca catgcagcac cacgtgaaat cgaatttaaa gataaattac
     4981 ctaaaacacg gtcaggtaaa attatgagac gtgtattaaa agcttgggaa ttaaatttag
     5041 atgctgggga tttaagtaca atggaataat gacatgaatg ttattgaaga tttttttcga
     5101 agaataaagg gtgacaacat atttcatgtc aatgtttaaa taatcgttta ctttacgata
     5161 agcaatataa agaactgtta acttgtgtca tatcatttcg tagaaagcat ttgaaaatga
     5221 tgacataaca ataatggcat atctttatat tgctttttat ttttaatatg atctttggaa
     5281 gatgattatt ttaaataata gaaaaatata gttatcaata gtatcaagcg ctaaaagttg
     5341 tataatacaa aactttaata agtgaattta ttgcaaaaat gaaagcgcta acccgattta
     5401 gtcgacaagt ttttaacagt tcgttattat atgaatgtaa gtaaaaattt cttagctaca
     5461 acttacatat tataaatgca taaattaaac aaaaaggggc gaaaaaagtt gactcattta
     5521 tcagatttag atattgcgaa tcaatcaaca ctacaaccaa ttaaggatat tgctgcatca
     5581 gtaggtattt cagaggatgc attagaacct tatggtcatt acaaagctaa aatcgacatt
     5641 aataaaatta cgccaagaga aaacaaaggg aaagttgttt tagtaactgc gatgagccca
     5701 acaccagctg gtgaaggtaa atcaacggtt acagttggtt tagctgatgc attccatgag
     5761 ttaaataaaa acgttatggt tgcattaaga gagcctgctt taggaccaac atttggtatc
     5821 aaaggtggtg cgactggtgg tggttatgcg caagtcttac ctatggaaga tatcaactta
     5881 catttcaacg gagatttcca tgcgattaca actgcaaata atgcattgtc tgcgtttatc
     5941 gataatcata ttcaccaagg taacgaatta ggaatcgatc aaagacgtat tgagtggaaa
     6001 cgtgtattag atatgaatga tcgtgcactt agacatgtaa acgttgggtt aggtggacct
     6061 acaaatggtg taccacgtga agatggcttt aatattacag tagcgtctga aattatggcg
     6121 attttatgtt taagtagaag tattaaagac ttaaaagata aaattagtcg tattactatt
     6181 ggttacacta gagatcgcaa gccagttaca gttgcagatt taaaagtgga aggtgcactt
     6241 gcaatgattt taaaagatgc aataaaacca aacttagtac aatcaattga agggacacct
     6301 gcattagttc atggtggacc atttgcgaat atcgcacacg gttgtaactc aattttagca
     6361 actgaaacag cacgtgattt agctgatatc gttgtaacgg aagctggatt tggttcagac
     6421 ttaggcgctg aaaaattcat ggacattaaa gtgcgtgaag caggatttga tccggcagct
     6481 gtcgttgttg ttgcgacaat tcgtgcgtta aaaatgcatg gtggtgtagc gaaagataat
     6541 ttaaaagaag aaaatgtaga agcagtaaaa gcaggaattg ttaatttaga gcgtcatgtt
     6601 aataatatta aaaaattcgg tgtagaaccg gttgttgcaa ttaatgcatt tatacatgat
     6661 accgatgcag aagtagaata tgtaaaatct tgggctaaag aaaataacgt acgaattgcc
     6721 ttaactgaag tttggaaaaa aggtggtaaa ggtggcgttg acttagcaaa tgaagtatta
     6781 gaagtcattg atcaacctaa ttcatttaaa cctttatatg aattagaatt accattagag
     6841 caaaagattg aaaagattgt gactgaaatc tatggcggtt caaaagtaac gtttagcagt
     6901 aaagcgcaaa aacaattaaa acaatttaaa gaaaatggtt gggataatta cccagtatgt
     6961 atggcgaaaa cacaatattc attctcagat gatcaaacgt tgttaggtgc accatcagga
     7021 tttgaaatta caattcgtga attagaagcg aaaacaggtg caggatttat cgtagcgttg
     7081 acaggtgcaa tcatgactat gcctggttta cctaaaaaac cagcagcatt aaacatggat
     7141 gttactgatg atggtcatgc aattgggtta ttctaataaa tcatgtcaat tgtttaataa
     7201 agataagtaa atagtttaat agaccggact gttggagatg cattatttca gcagttcggt
     7261 tttttgctgt gctaaaaata gattcaattt ggcgaatcta acgacaatgt ttgaaggtgg
     7321 ttaattaatg tatatgaaga taaaaagtgg gcttgaagaa taggaaagcg atgcaatgaa
     7381 tattccatat taaaaaaaaa taataaaata ggttgcaata tttaattggg atgcgctaca
     7441 attaacacta ataataggag ttgttaacaa catgaacaaa catcacccaa aattaaggtc
     7501 tttctattct attagaaaat caactctagg cgttgcatcg gtcattgtca gtacactatt
     7561 tttaattact tctcaacatc aagcacaagc agcagaaaat acaaatactt cagataaaat
     7621 ctcggaaaat caaaataata atgcaactac aactcagcca cctaaggata caaatcaaac
     7681 acaacctgct acgcaaccag caaacactgc gaaaaactat cctgcagcgg atgaatcact
     7741 taaagatgca attaaagatc ctgcattaga aaataaagaa catgatatag gtccaagaga
     7801 acaagtcaat ttccagttat tagataaaaa caatgaaacg cagtactatc actttttcag
     7861 catcaaagat ccagcagatg tgtattacac taaaaagaaa gcagaagttg aattagacat
     7921 caatactgct tcaacatgga agaagtttga agtctatgaa aacaatcaaa aattgccagt
     7981 gagacttgta tcatatagtc ctgtaccaga agaccatgcc tatattcgtt tcccagtttc
     8041 agatggcaca caagaattga aaattgtttc ttcgactcaa attgatgatg gagaagaaac
     8101 aaattatgat tatactaaat tagtatttgc taaacctatt tataacgatc cttcacttgt
     8161 aaaatcagat acaaatgatg cagtagtaac gaatgatcaa tcaagttcag tcgcaagtaa
     8221 tcaaacaaac acgaatacat ctaatcaaaa tacatcaacg atcaacaatg ctaataatca
     8281 accgcaggca acgaccaata tgagtcaacc tgcacaacca aaatcgtcaa cgaatgcaga
     8341 tcaagcgtca agccaaccag ctcatgaaac aaattctaat ggtaatacta acgataaaac
     8401 gaatgagtca agtaatcagt cggatgttaa tcaacagtat ccaccagcag atgaatcact
     8461 acaagatgca attaaaaacc cggctatcat cgataaagaa catacagctg ataattggcg
     8521 accaattgat tttcaaatga aaaatgataa aggtgaaaga cagttctatc attatgctag
     8581 tactgttgaa ccagcaactg tcatttttac aaaaacagga ccaataattg aattaggttt
     8641 aaagacagct tcaacatgga agaaatttga agtttatgaa ggtgacaaaa agttaccagt
     8701 cgaattagta tcatatgatt ctgataaaga ttatgcctat attcgtttcc cagtatctaa
     8761 tggtacgaga gaagttaaaa ttgtgtcatc tattgaatat ggtgagaaca tccatgaaga
     8821 ctatgattat acgctaatgg tctttgcaca gcctattact aataacccag acgactatgt
     8881 ggatgaagaa acatacaatt tacaaaaatt attagctccg tatcacaaag ctaaaacgtt
     8941 agaaagacaa gtttatgaat tagaaaaatt acaagagaaa ttgccagaaa aatataaggc
     9001 ggaatataaa aagaaattag atcaaactag agtagagtta gctgatcaag ttaaatcagc
     9061 agtgacggaa tttgaaaatg ttacacctac aaatgatcaa ttaacagatt tacaagaagc
     9121 gcattttgtt gtttttgaaa gtgaagaaaa tagtgagtca gttatggacg gctttgttga
     9181 acatccattc tatacagcaa ctttaaatgg tcaaaaatat gtagtgatga aaacaaagga
     9241 tgacagttac tggaaagatt taattgtaga aggtaaacgt gtcactactg tttctaaaga
     9301 tcctaaaaat aattctagaa cgctgatttt cccatatata cctgacaaag cagtttacaa
     9361 tgcgattgtt aaagtcgttg tggcaaacat tggttatgaa ggtcaatatc atgtcagaat
     9421 tataaatcag gatatcaata caaaagatga tgatacatca caaaataaca cgagtgaacc
     9481 gctaaatgta caaacaggac aagaaggtaa ggttgctgat acagatgtag ctgaaaatag
     9541 cagcactgca acaaatccta aagatgcgtc tgataaagca gatgtgatag aaccagagtc
     9601 tgacgtggtt aaagatgctg ataataatat tgataaagat gtgcaacatg atgttgatca
     9661 tttatccgat atgtcggata ataatcactt cgataaatat gatttaaaag aaatggatac
     9721 tcaaattgcc aaagatactg atagaaatgt ggataaagat gccgataata gcgttggtat
     9781 gtcatctaat gtcgatactg ataaagactc taataaaaat aaagacaaag tcatacagct
     9841 gaatcatatt gccgataaaa ataatcatac tggaaaagca gcaaagcttg acgtagtgaa
     9901 acaaaattat aataatacag acaaagttac tgacaaaaaa acaactgaac atctgccgag
     9961 tgatattcat aaaactgtag ataaaacagt gaaaacaaaa gaaaaagccg gcacaccatc
    10021 gaaagaaaac aaacttagtc aatctaaaat gctaccaaaa actggagaaa caacttcaag
    10081 ccaatcatgg tggggcttat atgcgttatt aggtatgtta gctttattca ttcctaaatt
    10141 cagaaaagaa tctaaataat taactaaata tagcatatgt atgattaact ttgtagacaa
    10201 tgtgaaagca attaatttat aaactattga ttggtttaat ggctttcctt tagagtaaat
    10261 aaaaagaaca gcagtgagaa attttctaat tgaaaataat cttactgctg tttttaatat
    10321 ttggattcat tgttgtggtt actttaaaaa gtgagcatca attaacgctt ttttcgattt
    10381 aacaaatgtg atttaatatc atattttaat gcgtcgttgt attctttttc agtgatttga
    10441 tcttcgatta acatacgctt taatacataa tgttgtcttt gaatactata tttcaaatct
    10501 ttatccgatt ttaacgttcc atctttttcg tagggtgtat agccatatgg gctttgcaac
    10561 aaaccgataa ggtatgcaga ttgtgcaatt gataaatctt ttggtggaat accaaataga
    10621 ctatatgaag cggatgcaat tccggaaata ttagcgccat tataatctct accgaaggga
    10681 actatattta aatatgtata tataatttca tcttttgaga gtaggtgttc taatctaatt
    10741 gctaggcgaa gttcatttgc ttttctacta tatgtttttt cgttggtaag aacttgattt
    10801 ttaacaagtt gttgtgtaat tgtgctacca cctgaacttt gatcagtatt aaaaatatct
    10861 tgtatcattg ctcttaaaat cgcctttggt aagatgccat catgtttata aaataaagtg
    10921 tcttcagatg acgttaatgc tttaatgaca tttggacttg atgttttagg gcctataatg
    10981 agtgagtttt gagaatggtt atactcatat aataaatttt tgttattatg atctaataat
    11041 tcatcgccag gtatttgtcg aacttttttt attaaagcat catctgataa tgagtcggac
    11101 gttttagtta aatgatgaaa atataaagac atcgcaatca cagcgatagc aacgatggca
    11161 atgaaaatca taaatataaa aacaatgaca tgtttgatag ctttatatat cttttcaaaa
    11221 tgatatatac gatgattcaa ttgatgattg ttgtcttgat tcgtcatctt taacctccta
    11281 aattacgtat agtataccat atttaagcaa tttgactttt attttaacgt taattataat
    11341 tagggaaaat gcataaagaa taggataaaa ggacaagtaa tattatttga aatcatcaga
    11401 gagctagtgg tcgctgtgaa ctagtgtttt taataatatg aatacacctt tataactaac
    11461 atgtcctacg tttattaatg aacgtatcat taatagagtg aacattatct tatgattatg
    11521 tttaagtttg gtggtaacgt gcaatcgcgc ccttacatat ttgttgtaag ggtttttttt
    11581 acgttcaaaa aactttaaag tattttaaca agtattcaat aaataattat aaataggagg
    11641 aaataataat gacgaatgta ttaattgaag atttaaaatg gagaggtctt atttatcaac
    11701 aaactgatga acaaggtatt gaagatttat taaataaaga acaagtgacg ttatactgcg
    11761 gtgccgatcc aacggcagat agtttacata ttggtcactt actaccattc ttaacattaa
    11821 gacgttttca agaacatgga catcgtccta tcgttttaat tggcggtggt acaggtatga
    11881 ttggtgatcc atcaggtaaa tcagaagaac gtgtgctaca aacagaagaa caagtagata
    11941 aaaatatcga aggtattagt aagcaaatgc acaatatttt tgaatttgga acagaccatg
    12001 gtgcagtgct tgttaataat agagactggt taggacaaat ctcattaatt agttttttac
    12061 gtgactatgg taaacacgtc ggcgttaatt acatgttagg taaagattca atccaaagtc
    12121 gtttagaaca tggtatttca tatacagaat tcacatacac gattttacaa gctattgatt
    12181 tcggtcattt gaatagagaa ttgaattgta agattcaagt aggtggatca gatcaatggg
    12241 gtaatatcac aagtggtatt gaattaatgc gtcgtatgta tggtcaaaca gacgcatacg
    12301 gtttaactat tccgcttgta actaaatcag atggtaagaa atttggtaag tctgagtcag
    12361 gtgctgtttg gttagatgct gaaaaaacaa gtccttatga attttatcaa ttctggatta
    12421 atcaatcaga cgaagatgta attaaattct taaaatactt tactttctta ggaaaagaag
    12481 aaattgatcg cttagaacaa tctaaaaatg aagcaccgca tttacgtgaa gctcaaaaaa
    12541 cattagctga agaagtaact aaatttattc atggtgaaga tgcattaaat gatgcaatcc
    12601 gtatttcaca agcattattt agtggtgatt taaaatcatt atcagcgaaa gaattaaaag
    12661 atggatttaa agatgtgcct caagtgacat tatcaaatga cacaacaaat atcgttgaag
    12721 tccttattga aacaggcatt tctccttcta aacgacaagc acgtgaagat gttaacaatg
    12781 gtgcgattta tattaatggt gagagacaac aagatgttaa ttatgcttta gcaccagaag
    12841 ataaaattga tggcgaattt acgattattc gtcgcggtaa gaaaaaatac ttcatggtta
    12901 actatcaata aatataattg catagctaaa taaattagag cctactcata ttcattccta
    12961 agaatgtaat gagtaggctc ttaatgtact tttctgtctg taaattatct aaagaaatct
    13021 ctatcgcctt ggccaggtga ttgacgttct gatcggcttt gacgttttgg ttgttctttt
    13081 tgttgtttta atttcacttt aacttctttt gttttaccat cacggataac ggtaacagtg
    13141 actgattcac caggtttttt attttcatat aaatagcttc ttaaatcaac atcatcttta
    13201 attttcttgc catcaatttc tgtaataata tcaccttttt taagatcaat atcactatca
    13261 gctttggcga cataaatacc gtcttctcta tcagtatgaa gttgctcgcg ctcttcttca
    13321 ggaatatctt tcaaattaat taaaccaata ccaatcgaag ggcggtcaat tttaccatgt
    13381 tttacaagtt gttcaattgt tactttaact tcattacttg gaatagcaaa cccgatacct
    13441 tcaacttgtg tcgcagcaat tttcattgag ttaataccaa ctaaattacc attaatatct
    13501 actaatgcgc cacctgagtt acctgggtta atagcagcat ctgtttgaag aacgctaact
    13561 tttgtattgc caccagttgt ctcagcgtca atcgtacgtt cgcttgctga aatgatacca
    13621 gatgttacag agttagcaaa ttgtaatcct aatgggttac ccattgcgaa tacgctatcg
    13681 ccagtttgta cttttgaaga gttggcaaat tgaatcgctt taataccttt tgtattttca
    13741 attttaagta cagcaatatc agttactgca tctttaccaa ctaatttcgc tttaacttgt
    13801 tttttattat gtaattggac tctaatttca tttgcgccat caataacatg attgtttgta
    13861 acgatataag ctgaattgtt gtttatttga tagataacac ctgaacctac tccagcttca
    13921 gatggtttag atgatttgcc ttttaataag tcgtctacac ttgatgcttt ttgcatgtta
    13981 ataactccaa caattgtagg ggagacagat tttatcattt catgaacggt accgaatttc
    14041 ttgctttgac cgtctaattg attgccacct ttattatttg ttgtctgaac agttgaacca
    14101 tctttattta aaattgtact atttaatact ttgcctatac caagtactag aagtgcacca
    14161 ataattccag caatcaatgc aacgatgact gttttaaacc atggaaattt aggtcttctg
    14221 tatctaggtg tttggctatg gtttgttgta gaatgatctg tatgattaaa atctgacata
    14281 cttaacctcc attatatgat ttatatatgc tttaattatg tctttttttt ataattattg
    14341 ctagtaatac actatacaac tttagatgta ttttcagact atcttgttct agtaaaaata
    14401 tagtgataaa atctagttgt tataaacaaa taagttaagt aggtggcaca atgtattcag
    14461 tgattagtaa gattttgaat ttcattttag ttaaaatgtc aaaatcatta tacgtaatcg
    14521 gtaaagataa tattcctaag gatagtaaat atgtcgtaac ttgtacgcat gaaagttata
    14581 acgaagtcat tatgctaggt atggcattat atcctaatca aattcattat atggctaaaa
    14641 aagaattatt caaaaataaa tggattggta aatttttaac gtctttaaat gcatttcctg
    14701 tagatcgtga aaatccagga cctagtactt taaaacgccc aatcaatttg ttgaaagata
    14761 ataagacagt tggtatcttc ccaacaggtt ctcgcacatc gcaagaaggt gcacctttga
    14821 aacgtggtgc ttcaacaatt gcaatgttaa gtaagtcacc aatattacct gttgcttatg
    14881 ttggtcctac taagattcac ggtcttttaa caggacaagc atacataaat attggcaaac
    14941 caatcgatat taacgattta cctaaagatt taaaacgaaa cgagcgtatc gattatatta
    15001 caaaagagat tgaaacacga acagcaaagt tacaacaaga attgcatgaa attgtaaaaa
    15061 gtttataaaa taacttgagg tagaaatata gctggttagt aagcctaggt gactataacg
    15121 gagtatcgaa gtagttattc tagttacatt agtaatcttt atatttctac attattttaa
    15181 gaaggatgac attcaatgta taaatttttc caaaatttag ggcgttcgtt aatgttacca
    15241 gttgccattt taccggctgc agcaattatt gccggaattg gtaatacgtt aaatgcactt
    15301 catgcgacgc cgaaaatcgc aatgttcttt actacggtag gtactacgat tttagagcaa
    15361 ttaggtattt tatttgcaat tggtgtagct ataggaatgg caaagaaaaa tgatggtgta
    15421 gtcgcgcttg cagctacgtt aggatatttt ttagttacag ttgtattatc accaatgaaa
    15481 ttagcgcctt tattaggaat gaaagcttcg gaaataaatt cagcttttga aaagatgaat
    15541 aatggcaatg tctttgttgg tattgtaata ggtttaatag cagcttatgc atacaataag
    15601 tttagtgaaa cagaattacc attagcatta tcatttttta gtggtaaacg tctagttcca
    15661 attatgactg cattttactg tacattttta gttgtcatat tgttattctt atggccacta
    15721 ctttattcat ggattgtaaa atttggtgaa tctatagtcg gtttaggatc gtttggagct
    15781 ttcatatatg gtgttgccaa tagattatta attcctacag gattacatca tgcactgaat
    15841 agtgtgttct ggtttgatac gattggtatt aatgatattg gtaaattcca aagtggcaaa
    15901 gatgctataa aaggtattac aggacgctat caagcgggat tcttcccaat catgatgttc
    15961 ggtattccag cggctgcgtt agcaatgtat catacagcta aaactactca gaaaaaacaa
    16021 gtttacggtt ggttcctagc gagttcagtt gcagcatttt tcgttggtgt tacagagccg
    16081 attgagtttg catttatgtt tgttgcgcca atcttatatg ttgttcatgc gttattaaca
    16141 ggattatctt tatttatcgc agctacattc cattggacag ctggattttc atttagtgca
    16201 ggtttgatag attatgtgtt atcactaatt aatccagtat caaatcatcc attgatgttg
    16261 cttgttcaag gtgttgtatt tttcatctta tactatgtga ttttccgtgt agtaatccaa
    16321 gtatttaact tgaatacgat tggtagaggt gaaaatgaat tagttgaccc aacagttgta
    16381 aaagataata ttgctcctgg tgaaaatgat attaaacaaa gtaaatatca tcaacatgct
    16441 atacaaatat tagaaggttt aggcggtcaa gagaatattg ttaatttaac caattgtgca
    16501 acaaggttgc gtctagagtt aaaagacaca tctattattg ataaacagaa gattaaaaac
    16561 gccggtgcag ttggtgttac tgttaatggc aaacattcaa ctcaagttat tgtcggtaca
    16621 cacgtacagc aggttgctga tgaaattgaa aaacatttat aaaatttaaa cagctaaagc
    16681 gcaaatctaa cttcaatagg gtttgcgctt tttttagtca aatagtagaa tgatttatat
    16741 ctgaattttc agaaatggag tggggattgt gaagaaaatt ttatttgatg ttgatggcgt
    16801 ttttttaagt gaagaacgtt gtttcgatgt ctctgcatta acggtatatg aattattaat
    16861 ggataagtgt tatttaggtc ttcattcaca tatagattgg gaaactttga ctgataacga
    16921 tatacaagac attaggaatc gaatttttca aaaagataag attttaaaca aattaaaatc
    16981 gctagggctg aattcaaatt gggatatgtt atttatcgtt tttagtattc atttaattga
    17041 tattttgaaa aagttatcac atgatgaaat tgaggcattc atgtatcaag atgagcctgt
    17101 cgaattgaag ttgcaaaata taagcacaaa cttagcagac tgttttaatt taaatgaaca
    17161 actaccttta caatttttgg ataatgtaaa agttggtaaa aataatattt atgctgcttt
    17221 ggaagagttt gcaacaacag agttacatgt ttcggatgct actttattta gtttgaaggg
    17281 tgcattatgg acgttagcgc aagaagttta tcaagaatgg tatttaggat cgaagttgta
    17341 tgaagatgtt gaaaagaaaa tagcacgaac tacttttaag acaggttata tttatcaaga
    17401 aattattttg agaccagtag atgaagttaa ggtacttctg aatgatttaa aaggtgctgg
    17461 tttcgaatta ggtattgcaa caggtcgtcc ttatactgag actgttgtgc catttgaaaa
    17521 tttaggattg ttaccatatt ttgaagctga ttttattgca acagcaagtg atgttttaga
    17581 agcagagaat atgtatccgc aagcacgacc attaggaaag ccgaatcctt ttagttatat
    17641 cgcagcttta tatggtaata atcgcgataa atatgaatct tatatcaata agcaagataa
    17701 cattgtaaat aaagatgacg tatttatagt aggcgattcg ttagctgact tattaagtgc
    17761 tcaaaaaata ggtgcaacgt ttattggaac attaacaggt ttaaaaggta aggatgctgc
    17821 aggtgagtta gaagcgcatc atgccgacta tgttattaat catttaggtg aacttagagg
    17881 tgtactagat aatttgtaat ttgattgttg tttgacagca taacttgtag tgaatgattg
    17941 aaccaaaggt ttcatattga gttacaatga aattaataat gaaaaaatgc caagaagcaa
    18001 tggaagtaat ccaatgtctt cttggcattt tgaatttaca taaattgttt atgactgtac
    18061 cgtcaattca gttgtgaaaa tttgattgta ttcaccaact tgtttaagtt catcaattat
    18121 attgtttgaa acaggttgat caacggataa aatcattagc gcatctccgc ccgcttcagt
    18181 tctacctaaa gtcatagatg caatgttgat attgtattta cctaacaatg cgccagtttt
    18241 tcctaccata cctggagtat cattatgata tgacacaatt tgatattgat ttggctttaa
    18301 gtctacagaa aaattattaa ttctaacaat tcttggaccg aaacctgtaa agacagaagc
    18361 gccaacttta acggaatcgc tatcgtttga tagttctacc tctaagtagt tactaaaacc
    18421 tgtctctgct ttattatttt caatatttaa tgtcacttgt tgttgattta acaacattag
    18481 agcattgata atattgactt caggaccaag atcatctttt aaaatatgag taataattgt
    18541 acgtgttaat aaactactat caatagaggc taagtcgcca ccgtacgtaa ttttaataga
    18601 gcttggtgca ttgtacatta attgaatagc taattcacca actgtttggc ttaaattgat
    18661 gaatgatttt acagtatcat ctatattgct taagtccatt ttaggtgcat tcactgcatg
    18721 cgttacagta ccatcaatta aaatttcgat gatttcattt gaaacagaaa ttgccacttt
    18781 ttcttgagct tcgactgttg aagcacccaa atgaggtgta acaataattt tatcatgtgc
    18841 aacaagaggc gagtcagttg caggttcatg ttcaaacaca tcgatagctg cccgactaat
    18901 ttgtccttcg tctaatgctt ttattagcgc cttttcatca ataataccac cacgtgccac
    18961 attgattatt tgcaaactag gttttgcttt ggcaaaaaag acagcattaa ttaagccttt
    19021 tgttttaggt gttagtggtg tatgtaatgt aacgaaatca gaatgttggg caatctcatc
    19081 aactgttgcc ttcgtaatgc ttaaagattt tgctttttca tccgttaagt aagggtcaaa
    19141 agctagtatt ttcattccga aactttgcgc acgtttagca acacctaaac caattctacc
    19201 agcaccaatg acacctaatg ttttatgata aagctcagta cctttaaatg catttcgatt
    19261 ccattcttta tttgtaagtg attggtgtgc ttgcggaata tttcgtgcca ttgataataa
    19321 cattgccagt gtatgttcag tagctgaaat cgtattacca tctggggcat taataactaa
    19381 aatacctttt aatgttgcag catcaatatt aatattatct acaccaacac cggcgcgtgc
    19441 gattactttt aaagaatcag cagcatttat gatattttca gtaaccgtag tttgactacg
    19501 aacgattaaa gcatggtatg aaggtataat tttgattaat gcttcttcgg acaagccagt
    19561 ttgaatatct acattgaatt gttcgtgatc taataatgct ttgataccat cttttgatat
    19621 gggatctgca acgagtacat taaattgctt catgaataac ctccatatat tttgatatac
    19681 ctttaccgat atagttaact ttacggtgtt cagttaaaat aatttctaaa gcagatacta
    19741 ccgataaaat atcgaaagga ctaattttcc ccatatgacc aattcttaaa atttgacctt
    19801 taagatggcc ttgaccacca gcaattgtta ttttaaagcg atttttaagt tcatctttga
    19861 ttattttcac ttcatcattt gtattaggtt tgaatgctgt aaccgttgga gatgcatctt
    19921 tatcattgac taataaagtt aaatctaatg cttttaaggc gcttcttaat gcatttctaa
    19981 ttgcatagtg tcgtgctatt acgtgattga aaccttctgc ttttacggtt tcaacgtatg
    20041 catttacacc tctaaataaa gacacatttg gtgtgaacgg tgtagaattg tcagcttgtg
    20101 acgaaatgta tttatttaag tctagataaa attttggcgt agttacttct ttgaaatgtt
    20161 cttttgcacg gtggctataa gctacaaaag ctaatcctgg aggtaacata attgcttttt
    20221 gactaccaga aacaagtaca tcaattttat ctttgttaat gtcaacatca acagcaccaa
    20281 tgcaacttac gccgtcaact acaaaataaa tattactatt aaattgatta atggcatttc
    20341 ctaactcgtg aatagggtgt agcactgtcg tagatgtttc gcaatattga ctaaatactg
    20401 ctttaacttc aacatttaaa gttgaaagga aattgatgaa atctttgaca tctacagctt
    20461 ctccccatgt tacgtcataa atatgcacat ttttgtaata agtttgtgca atttgtttaa
    20521 atcggttacc aaaggcacct gaaacaatga caacgaagtg atcttcaggg tttacaatgt
    20581 tcaacatact agcctccaag acgcttgtac cgctagatgt taaaataagt acatcatttt
    20641 gactcccaaa tattggcttt agaccttgaa atgcttgttg tgcgatgtct tcaaaatctt
    20701 tagaacgatg accaaccata ggtgcttgaa tttctctcat aatggcatca ggtacagggg
    20761 ttgggccagg tgttaataac aacggttgat gataatacat ttatatgcct cctataaata
    20821 agatttcact attttagcaa attttctgaa aatttaaaag cctaaaaaag tgataacgct
    20881 atattgtcga acaaacgtta tcacttaacg attttatttg gcctgaatga tgggatagat
    20941 ttttatgtcc acattatttc ttacagcatt tgaaatcatg caattattat ctgcaattgt
    21001 tatcaatttt ggtaatcgct tttctaattg tgctatttga tcacttggaa tttgaatttg
    21061 aggatggtgc acaatttttg acatactgaa ttttccgtta tttaaacaag ctgttccaat
    21121 cgattgttgt tcaattgaaa tatctgtgaa ctttgcacgt tcaagagtag ctgctaatga
    21181 gatgatataa catgatgaag cggctgatac taacatttca tcgggatttg ttcctatacc
    21241 aacaccacct aaagaagcag gtatagaaat attctctgaa agtatgtcgc cttgaacgtt
    21301 tccgacattg ttacgaccac cttgccaaga agtttggact ttaaagtcat gttgatgcaa
    21361 ttgctttaac ctccaatata attgtgatag tttaatttta gaatagttta tcaaagttta
    21421 acagaaaggt gacttatcaa tgactctgaa taaactgaaa gatgaattac aaattgtttc
    21481 gcaccgtgga ttgccgagtg attttcctga aaatacaatg gtcggttatc gagaggtaat
    21541 ggggctcaat gttgctatgt tagaaataga tgttcatttg accaaagacc aacattttgt
    21601 tgtgatacat gatgaaacaa ttgatagaac atcggatggt aaggggcgta ttgctgatta
    21661 cacattatcg caattaaaat catttgattt tggtagttat aaagatgttg cttttaaggg
    21721 agaacgaata cccacgttag atgaagtact ttcattatgc ttaaagtatg ataaaaagct
    21781 attgattgaa ttgaaaagtc cgaatttata tccagaaata gaatgtaaat tgctggcatt
    21841 tttggaagaa aagaaagtcg atgcgacaca agtagttata caatcttttg atatcgaatg
    21901 catcgaaaag ttaaacacat tgggcagtat atatgaatta ggtgtgcttt gtagtaaacg
    21961 taattattgg tataaaaagc caaacttttc aagaattgct caaattgcta gttatgttaa
    22021 tcctaattat gcattagtga ccagaaaatt tgttgataaa gcacatcatc atcaattaca
    22081 ggttatgcca tacacagtta ataaattgaa aactggagaa aaattaagac aaatgggtgt
    22141 agatggatta attacagata atccgaaatt atttattaag tgagacgatg aatggtaatt
    22201 tgatagaggg atgaggattc gtatatcgca attgataatt ttaaaaacta atgatgaaat
    22261 actaaagata attaaacaaa taagacttgt aaattctaag ttgattgtaa aatagggtag
    22321 ctccatataa ttttgaatct atcattttta aattaataaa aaaaacaccc acaattgtgg
    22381 gtgtttgtat tgaacgtatt atcttgagta gtactcaacg attaattgtt cgttaatttc
    22441 agcaggtaat tcgctacgtt ctggtaaacg tacgaaagta ccagttaagc tgtcagcatc
    22501 aaagtttaag tactcaggta cgaaattgtt gatttcaact gattcaacga tgatgtttaa
    22561 tttttgagat ttttcacgaa ctgaaattgt ttgaccaggt ttaacagaat aagatggaat
    22621 atcaacacgt ttaccatcta ctaagatatg accgtggtta actaattgac gtgcttgacg
    22681 acgagtacga gctaaaccta atgaataaac aacagcgtct aaacgacttg ctaataagat
    22741 catgaagttt tcaccgtgta caccgaattt tttaccagcg atgtcaaatg tgttacggaa
    22801 ttgtctttca gtcattccat ataagtaacg taatttttgt ttttcacgta attgtaaacc
    22861 atattctgat aattttttac gttggtttgg accatgttgt cctggtgcgt aaggacgttt
    22921 ttctaattct ttaccagtac cgcttaaaga gatacctaaa cgacgagatt ttttccagtt
    22981 tgaacctctg aatcgagcca taataagact cctcctttaa ttcttttttg ttgttatgaa
    23041 taaacaaaaa agagtgttgc atgcttacta agatatattg ttttatgtgt cttcacctta
    23101 tagcgacagt tacgtgacac gtccgcattg ggaacaacat agcgctcaat aatatacaac
    23161 tgctactttc gttaattcac acaaagatta ttgtaacatt ttcctgctaa ctgtcaacac
    23221 tttattgaaa atgctgatgt ccttttatgc gagttgcttt tcaataattt taacaattgc
    23281 ttcaagatgt tctttgtcat tgtcatcaaa tcgatccgtt ataggggcat cgatatctaa
    23341 gacgccgata attttatcat ctttgaaaat tggaacgaca atctcagact tactattagc
    23401 atcacaagcg atatgtcctt cgaattgatg aacatcagct acaatttgtg tacgacgttc
    23461 tgaaacggct gtaccacata caccttttcc aattggaatg tggacacaag cggggtgtcc
    23521 ttggaaaggt ccaagtataa gttcattttg ttccaataag taaaagccaa cccaattaat
    23581 ttgatctaga ttatcattta ataaggcaga catgttactt aaaatagcaa tcatgtgatg
    23641 ttcatcttca ataaggcttg ctgcttgttt ctttaataat gtgtagtttg ttgggttaat
    23701 tgttgtcata tttaaaacct cttattttca
//
LOCUS       DALAGD010000041        23069 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.041, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000041 DALAGD010000000
VERSION     DALAGD010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 23069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23069
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.041"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(140..553)
                     /locus_tag="O2701_001289"
     CDS             complement(140..553)
                     /locus_tag="O2701_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293192.1"
                     /translation="MKPTKVILKDASYLHSKTSITFILKDVVIEEDNKIYYFDTSATF
                     EDQEVKFEFALFDSDMDNLKHMDCDNPVTEIYFIEPDLHFTIIDFNQELLCIYIDFDS
                     GLRHSNMATESGISLRINVAKSDFTKFINELASLH"
     gene            complement(582..692)
                     /locus_tag="O2701_001290"
     CDS             complement(582..692)
                     /locus_tag="O2701_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293193.1"
                     /translation="MDGNNQIDVCFFVLVNNYYTMSIYPRIINSNGDYAT"
     gene            complement(778..1509)
                     /locus_tag="O2701_001291"
     CDS             complement(778..1509)
                     /locus_tag="O2701_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCZ4293194.1"
                     /translation="MHELTIYHFMSDKLNLYSDIGNIIALRQRAKKRNIKVNVVEINE
                     TEGITFDECDIFFIGGGSDREQALATKELSKIKTPLKEAIEDGMPGLTICGGYQFLGK
                     KYITPDGTELEGLGILDFYTESKTNRLTGDIVIESDTFGTIVGFENHGGRTYHDFGTL
                     GHVTFGYGNNDEDKKEGIHYKNLLGTYLHGPILPKNYEITDYLLEKACERKGIPFEPK
                     EIDNEAEIQAKQVLIDRANRQKKSR"
     gene            complement(1511..2824)
                     /gene="murT"
                     /locus_tag="O2701_001292"
     CDS             complement(1511..2824)
                     /gene="murT"
                     /locus_tag="O2701_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit MurT"
                     /protein_id="HCZ4293195.1"
                     /translation="MRQWTAIHLAKLARKASRAVGKRGTDLPGQIARKVDTDILRKLA
                     EQVDDIVFISGTNGKTTTSNLIGHTLKANNIQIIHNNEGANMAAGITSAFIMQSTPKT
                     KIAVIEIDEGSIPRVLKEVTPSMMVFTNFFRDQMDRFGEIDIMVNNIAETISNKGIKL
                     LLNADDPFVSRLKIASDTIVYYGMKAHAHEFEQSTMNESRYCPNCGRLLQYDYIHYNQ
                     IGHYHCQCGFKREQAKYEISSFDVAPFLYLNINDEKYDMKIAGDFNAYNALAAYTVLR
                     ELGLNEQTIKNGFETYTSDNGRMQYFKKERKEAMINLAKNPAGMNASLSVGEQLEGEK
                     VYVISLNDNAADGRDTSWIYDADFEKLSKQQIEAIIVTGTRAEELQLRLKLAEVEVPI
                     IVERDIYKATAKTMDYKGFTVAIPNYTSLAPMLEQLNRSFEGGQS"
     gene            3116..3616
                     /gene="ftnA"
                     /locus_tag="O2701_001293"
     CDS             3116..3616
                     /gene="ftnA"
                     /locus_tag="O2701_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-type ferritin FtnA"
                     /protein_id="HCZ4293196.1"
                     /translation="MLSKNLLEALNDQMNHEYFAAHAYMAMAAYCDKESYEGFANFFI
                     QQAKEERFHGQKIYNYINDRGAHAEFRAVSAPKIDFSSILETFKDSLSQEQEVTRRFY
                     NLSEIARQDKDYATISFLNWFLDEQVEEESMFETHINYLTRIGDDSNALYLYEKELGA
                     RTFDEE"
     gene            complement(3698..3790)
                     /locus_tag="O2701_001294"
     CDS             complement(3698..3790)
                     /locus_tag="O2701_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293197.1"
                     /translation="MTKNTIISLENEKTQINDSENESSDLRKAK"
     gene            4005..4559
                     /locus_tag="O2701_001295"
     CDS             4005..4559
                     /locus_tag="O2701_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HCZ4293198.1"
                     /translation="MIQDAFVALDFETANGKRTSICSVGMVKVIDSQITETFHTLVNP
                     QDYFSQQNIKIHGIQPEDVENAPTFDYVFPYMMQFIADLPVVAHNAAFDMNVLHQSIQ
                     NIGLPTPNLTYFCSYQLAKRTVDSYRYGLKHMMEFYQLDFHGHHDALNDAKACAMITF
                     RLLKNYENLTYVTNIYGKNLKDKG"
     gene            complement(4627..5697)
                     /gene="dinB"
                     /locus_tag="O2701_001296"
     CDS             complement(4627..5697)
                     /gene="dinB"
                     /locus_tag="O2701_001296"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCZ4293199.1"
                     /translation="MTERRIIHIDMDYFFAQVEMRDNPKLKGKPVIVGGKASSRGVVS
                     TASYEARKYGVHSAMPMSQAHKLCPNGYFVTSNFGAYRETSAQIMSIFRSYTDKVEPM
                     SLDEAYLDITELVRPDLPASKIAQYIRKDILEQTHLTASAGVSYNKFLAKLASGMNKP
                     DGMTVIDYQNVHDILMTLDIGDFPGVGKASKKVMHDNGIFNGRDLYEKTEFELIRLFG
                     KRGRGLYNKARGIDHSEVKSSRVRKSVGTERTFATDVNDDEEILRKVWELSGKTAERL
                     NKLQKSAKTVTVKIKTYQFETLSKQMSLRDSVSSEEDIYNIAYLLYNDLKDPDVPIRL
                     IGVTVGNLEQSTYKNMTIYDFI"
     gene            complement(5944..6474)
                     /locus_tag="O2701_001297"
     CDS             complement(5944..6474)
                     /locus_tag="O2701_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HCZ4293200.1"
                     /translation="MHKIDLTTNNFQMRRFIILQLVIALFVILFTYKWALGVTAVVDQ
                     NIIINLVYGFAGFIILLILHELIHRALFLLFKKDSKPMFNIKKDRILFQTADACFNKW
                     QFSIIMLSPLILLSTGLLILIKVFGYSSLIFMFSMHTAYCFIDILLVALTISSSFKYV
                     QQDEDSIYLYHQKPTQ"
     gene            complement(6644..8005)
                     /gene="rlmD"
                     /locus_tag="O2701_001298"
     CDS             complement(6644..8005)
                     /gene="rlmD"
                     /locus_tag="O2701_001298"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCZ4293201.1"
                     /translation="MQAIAKNDIKTGTVVDLTHEGHGVVKIDRFPIFIPQALINEQIE
                     YKIIKVKKNFAIGKLLNINTRSENRVAPPCIYYERCGGCQLQHLSYEAQLEMKKEQVI
                     NLFQRKAHFDNSKINDTVGMTDPWRYRNKSQIPVGKNEQNEVIMGFYRQRSHDIIDME
                     SCLIQDSQHQEVMNEVKSILKDLNVSIYQEQLKKGLMRHLVVRTGYHTDEMMIIFVTN
                     GKKWPQKNAVVEKILDAFPNVTSIKQNINDSHSNVIMGRQSITLYGKDTIIDQLTDST
                     FKISDQSFYQINSEQTEKLYNKAIEYAQLTGNEVVLDTYCGIGTIGLYMAPLAKHVYG
                     VEVVPSAIEDAQQNATINQCNNTTFVCGKAEEVILQWKAQGIKPDVVMVDPPRKGCDE
                     TFIQTLLTLEPKRIVYISCNPATQQRDALLLAEKYQLEEVTPVDMFPQTTHVETVALF
                     NLK"
     gene            complement(8086..9033)
                     /locus_tag="O2701_001299"
     CDS             complement(8086..9033)
                     /locus_tag="O2701_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCZ4293202.1"
                     /translation="MRKRARIIYNPTSGKELFKRELPDALIKLEKAGYETSAYATEKI
                     GDATLEAERAMHENYDVLIAAGGDGTLNEVVNGIAEKPNRPKLGVIPMGTVNDFGRAL
                     HIPNDIMGALDVIIEGHSTKVDIGKMNNRYFINLAAGGQLTQVSYETPSKLKSIVGPF
                     AYYIKGFEMLPQMKAVDLRIEYDGNVFQGEALLFFLGLTNSMAGFEKLVPDAKLDDGY
                     FTLIIVEKSNLAELGHIMTLASRGEHTKHPKVIYEKAKAINISSFTDLQLNVDGEYGG
                     KLPANFLNLERHIDVFAPNDIVNEELINNDHVDDNLIEE"
     gene            complement(9616..9762)
                     /locus_tag="O2701_001300"
     CDS             complement(9616..9762)
                     /locus_tag="O2701_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293203.1"
                     /translation="MIADLDAIEGLFISIEVKLLLTLFIVHVNGKNINFINALALIIL
                     RQTV"
     gene            complement(9802..11229)
                     /gene="gatB"
                     /locus_tag="O2701_001301"
     CDS             complement(9802..11229)
                     /gene="gatB"
                     /locus_tag="O2701_001301"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HCZ4293204.1"
                     /translation="MHFETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAY
                     PGVLPVVNKRAVDWAMRAAMALNMEIATESKFDRKNYFYPDNPKAYQISQFDQPIGEN
                     GYIDIEVDGETKRIGITRLHMEEDAGKSTHKGEYSLVDLNRQGTPLIEIVSEPDIRSP
                     KEAYAYLEKLRSIIQYTGVSDVKMEEGSLRCDANISLRPYGQEKFGTKAELKNLNSFN
                     YVRKGLEYEEKRQEEELLNGGEIGQETRRFDESTGKTILMRVKEGSDDYRYFPEPDIV
                     PLYIDDAWKERVRQTIPELPDERKAKYVNELGLPAYDAHVLTLTKEMSDFFESTIEHG
                     ADVKLTSNWLMGGVNEYLNKNQVELLDTKLTPENLAGMIKLIEDGTMSSKIAKKVFPE
                     LAAKGGNAKQIMEDNGLVQISDEATLLKFVNEALDNNEQSVEDYKNGKGKAMGFLVGQ
                     IMKASKGQANPQLVNQLLKQELDKR"
     gene            complement(11242..12699)
                     /gene="gatA"
                     /locus_tag="O2701_001302"
     CDS             complement(11242..12699)
                     /gene="gatA"
                     /locus_tag="O2701_001302"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HCZ4293205.1"
                     /translation="MSIRYESVENLLTLIKDKKIKPSDVVKDIYDAIEETDPTIKSFL
                     ALDKENAIKKAQELDELQAKDQMDGKLFGIPMGIKDNIITNGLETTCASKMLEGFVPI
                     YESTVMEKLHNENAVLIGKLNMDEFAMGGSTETSYFKKTVNPFDHKAVPGGSSGGSAA
                     AVAAGLVPFSLGSDTGGSIRQPAAYCGVVGMKPTYGRVSRFGLVAFASSLDQIGPLTR
                     NVKDNAIVLEAISGADVNDSTSAPVDDVDFTSEIGKDIKGLKVALPKEYLGEGVADDV
                     KEAVQNAVETLKSLGAVVEEVSLPNTKFGIPSYYVIASSEASSNLSRFDGIRYGYHSK
                     EAHSLEELYKMSRSEGFGKEVKRRIFLGTFALSSGYYDAYYKKSQKVRTLIKNDFDKV
                     FENYDVVVGPTAPTTAFNLGEEIDDPLTMYANDLLTTPVNLAGLPGISVPCGQSNGRP
                     IGLQFIGKPFDEKTLYRVAYQYETQYNLHDVYEKL"
     gene            complement(12701..13003)
                     /gene="gatC"
                     /locus_tag="O2701_001303"
     CDS             complement(12701..13003)
                     /gene="gatC"
                     /locus_tag="O2701_001303"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HCZ4293206.1"
                     /translation="MTKVTREEVEHIANLARLQISPEETEEMANTLESILDFAKQNDS
                     ADTEGVEPTYHVLDLQNVLREDKAIKGIPQELALKNAKETEDGQFKVPTIMNEEDA"
     gene            13370..14908
                     /gene="putP"
                     /locus_tag="O2701_001304"
     CDS             13370..14908
                     /gene="putP"
                     /locus_tag="O2701_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCZ4293207.1"
                     /translation="MLTMGTALSQQVDANWQTYIMIAVYFLILIVIGFYGYKQATGNL
                     SEYMLGGRSIGPYITALSAGASDMSGWMIMGLPGSVYSTGLSAMWITIGLTLGAYINY
                     FVVAPRLRVYTELAGDAITLPDFFKNRLNDKNNVLKIISGLIIVVFFTLYTHSGFVSG
                     GKLFESAFGLDYHFGLILVAFIVIFYTFFGGYLAVSITDFFQGVIMLIAMVMVPIVAM
                     MNLNGWGTFHDVAAMKPTNLNLFKGLSFIGIISLFSWGLGYFGQPHIIIRFMSIKSHK
                     MLPKARRLGISWMAVGLLGAVAVGLTGIAFVPAYHIKLEDPETLFIVMSQVLFHPLVG
                     GFLLAAILAAIMSTISSQLLVTSSSLTEDFYKLIRGEEKAKTHQKEFVMIGRLSVLVV
                     AIVAIAIAWNPNDTILNLVGNAWAGFGASFSPLVLFALYWKGLTRAGAVSGMVSGALV
                     VIVWIAWIKPLAHINEIFGLYEIIPGFIVSVIVTYVVSKLTKKPGAFVETDLNKVRDI
                     VREK"
     gene            complement(14997..16196)
                     /locus_tag="O2701_001305"
     CDS             complement(14997..16196)
                     /locus_tag="O2701_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CamS family sex pheromone protein"
                     /protein_id="HCZ4293208.1"
                     /translation="MKRTLVLLITAIFILAACGNHKDDQAGKDNQKHNNSSNQVKEIA
                     TDKNVQGDNYRTLLPFKESQARGLLQDNMANSYNGGDFEDGLLNLSKEVFPTDKYLYQ
                     DGQFLDKKTINAYLNPKYTKREIDKMSEKDKKDKKANENLGLNPSHEGETDPEKIAEK
                     SPAYLSNILEQDFYGGGDTKGKNIKGMTIGLAMNSVYYYKKEKDGSTFSKKLDDSEVK
                     KQGKQMASEILSRLRENDDLKDIPIHFAIYKQSSEDSITPGEFITQATAEKSQTKLNE
                     WHNINEKSALLPSSTAADYDENLNNNFKQFNDNLQSYFSNFTQAVGKVKFVDKKPQRL
                     VVDLPIDYYGQAETIGITQYVTEQANKYFDKIDNYEIRIKDGNQPRALISKTKDDKEP
                     QVHIYSN"
     gene            complement(16209..18212)
                     /gene="ligA"
                     /locus_tag="O2701_001306"
     CDS             complement(16209..18212)
                     /gene="ligA"
                     /locus_tag="O2701_001306"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCZ4293209.1"
                     /translation="MADLSSRVNELHDLLNQYSYEYYVEDNPSVPDSEYDKLLHELIK
                     IEEEHPEYKTVDSPTVRVGGEAQASFNKVNHDTPMLSLGNAFNEDDLRKFDQRIREQI
                     GNVEYMCELKIDGLAVSLKYVDGYFVQGLTRGDGTTGEDITENLKTIHAIPLKMKEPL
                     NVEVRGEAYMPRRSFLRLNEEKEKNDEQLFANPRNAAAGSLRQLDSKLTAKRKLSVFI
                     YSVNDFTDFNARSQSEALDELDKLGFTTNKNRARVNNIDGVLEYIEKWTSQRESLPYD
                     IDGIVIKVNDLDQQDEMGFTQKSPRWAIAYKFPAEEVVTKLLDIELSIGRTGVVTPTA
                     ILEPVKVAGTTVSSASLHNEDLIHDRDIRIGDSVVVKKAGDIIPEVVRSIPERRPEDA
                     VTYHMPTHCPSCGHELVRIEGEVALRCINPKCQAQLVEGLIHFVSRQAMNIDGLGTKI
                     IQQLYQSELIKDVADIFYLTEEDLLPLDRMGQKKVDNLLAAIQQAKDNSLENLLFGLG
                     IRHLGVKASQVLAEKYETIDRLLTVTEAELVEIHDIGDKVAQSVVTYLENEDIRALIQ
                     KLKDKHVNMIYKGIKTSDIEGHPEFSGKTIVLTGKLHQMTRNEASKWLASQGAKVTSS
                     VTKNTDVVIAGEDAGSKLTKAQSLGIEIWTEQQFVDKQNELNS"
     gene            complement(18216..20408)
                     /gene="pcrA"
                     /locus_tag="O2701_001307"
     CDS             complement(18216..20408)
                     /gene="pcrA"
                     /locus_tag="O2701_001307"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HCZ4293210.1"
                     /translation="MNALLNHMNTEQSEAVKTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     LDEKDVSPYNVLAITFTNKAAREMKERVQKLVGDQAEVIWMSTFHSMCVRILRRDADR
                     IGIERNFTIIDPTDQKSVIKDVLKNENIDSKKFEPRMFIGAISNLKNELKTPADAQKE
                     ATDYHSQMVATVYSGYQRQLSRNEALDFDDLIMTTINLFERVPEVLEYYQNKFQYIHV
                     DEYQDTNKAQYTLVKLLASKFKNLCVVGDSDQSIYGWRGADIQNILSFEKDYPEANTI
                     FLEQNYRSTKTILNAANEVIKNNSERKPKGLWTANTNGEKIHYYEAMTERDEAEFVIR
                     EIMKHQRNGKKYQDMAILYRTNAQSRVLEETFMKSNMPYTMVGGQKFYDRKEIKDLLS
                     YLRIIANSNDDISLQRIINVPKRGVGPSSVEKVQNYALQNNISMFDALGEADFIGLSK
                     KVTQECLNFYELIQSLIKEQEFLEIHEIVDEVLQKSGYREMLERENTLESRSRLENID
                     EFMSVPKDYEENTPLEEQSLINFLTDLSLVADIDEADTENGVTLMTMHSAKGLEFPIV
                     FIMGMEESLFPHIRAIKSEDDHEMQEERRICYVAITRAEEVLYITHATSRMLFGRPQS
                     NMPSRFLKEIPESLLENHSSGKRQTIQPKAKPFAKRGFSQRTTSTKKQVLSSDWNVGD
                     KVMHKAWGEGMVSNVNEKNGSIELDIIFKSQGPKRLLAQFAPIEKKED"
     gene            complement(20405..21097)
                     /locus_tag="O2701_001308"
     CDS             complement(20405..21097)
                     /locus_tag="O2701_001308"
                     /EC_number="2.5.1.n9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenylglyceryl phosphate synthase"
                     /protein_id="HCZ4293211.1"
                     /translation="MYDIKKWRHIFKLDPAKHISDDDLDAICMSQTDAIMIGGTDDVT
                     EDNVIHLMSRVRRYPLPLVLEISNIESVMPGFDFYFVPTVLNSTDVVFHNGTLLEALK
                     TYGHSIDFEEVIFEGYVVCNADSKMAKHTKANTDLTTEDLEAYAQMVNHMYRLPVMYI
                     EYSGIYGDVSKVQAVSEHLTETQLFYGGGISSEQQATEMAAIADTIIVGDIIYKDIKK
                     ALKTVKIKESSK"
     gene            complement(21269..21571)
                     /locus_tag="O2701_001309"
     CDS             complement(21269..21571)
                     /locus_tag="O2701_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293212.1"
                     /translation="MQIEKLRGAALDELFDAILTLENREECYQFFDDLCTVNEIQSLS
                     QRLQVAKMIKQGYTYATIEQESGASTATISRVKRSLQWGNDAYTMILDRMNIETNE"
     gene            complement(21680..22975)
                     /gene="purB"
                     /locus_tag="O2701_001310"
     CDS             complement(21680..22975)
                     /gene="purB"
                     /locus_tag="O2701_001310"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCZ4293213.1"
                     /translation="MIERYSREEMSNIWTDQNRYEAWLEVEILACEAWSELGHIPKAD
                     VQKIRQNAKVNVERAQEIEQETRHDVVAFTRQVSETLGEERKWVHYGLTSTDVVDTAL
                     SFVIKQANDIIEKDLERFIDVLAEKAKNYKYTLMMGRTHGVHAEPTTFGVKMALWYTE
                     MQRNLQRFKQVREEIEVGKMSGAVGTFANIPPEIESYVCKHLGIGTAPVSTQTLQRDR
                     HAYYIATLALIATSLEKFAVEIRNLQKTETREVEEAFAKGQKGSSAMPHKRNPIGSEN
                     ITGISRVIRGYITTAYENVPLWHERDISHSSAERIMLPDVTIALDYALNRFTNIVDRL
                     TVFEDNMRNNIDKTFGLIFSQRVLLALINKGMVREEAYDKVQPKAMISWETKTPFREL
                     IEQDESITSVLTKEELDECFDPKHHLNQVDTIFERAGLA"
BASE COUNT         7008 a         4260 c         3295 g         8506 t
ORIGIN      
        1 gcctcttttt atcgtatata gcatgtccct ttttaaaatt acaagcataa atattcagac
       61 tcaaatatag ccacacgcct aaactacgga gggatgtggc tgtctttttt gattgtaatt
      121 acatatcaga tttaaatcat taatgtaaag aggctaattc attaataaat ttagtaaaat
      181 ctgatttagc aacatttatc cttaatgaaa taccagattc tgttgccatg tttgaatgcc
      241 ttaaaccaga atcaaaatca atataaatac aaagcagttc ttgattaaaa tcaataattg
      301 taaaatgtaa atcaggttct ataaaatata tttcagtaac aggattgtcg caatccatat
      361 gtttcaaatt gtccatgtca ctatcaaaga gtgcaaattc aaatttaact tcttgatctt
      421 cgaaagttgc actagtgtcg aaataataaa ttttattatc ttcttcgata actacatctt
      481 ttaaaataaa tgttatcgat gttttgctat gtaaataaga tgcatctttt aatatcactt
      541 tagtaggctt catcgtcaaa ttcctttcat cattcatttt ttcatgttgc ataatcacca
      601 ttactattga taattctagg atagatactc attgtataat aattattcac tagaacaaaa
      661 aaacacacat caatttggtt gtttccatcc aaaataatgt gtgtgtttat acatatatga
      721 ttaattacta aacttcatcg tttatcaata ttaaatccat tgatgcgatg ttcagagtta
      781 acgagatttc ttctgtctat ttgctctgtc tattaatact tgtttcgctt gtatttccgc
      841 ttcattatct atttctttag gctcaaacgg aataccctta cgttcacaag ctttttctaa
      901 cagataatca gtgatttcgt aatttttagg taaaattggt ccatgtaaat aagtacctaa
      961 taaattttta taatgaatgc cttctttttt atcttcatca ttattaccat aaccaaaagt
     1021 aacatgacca agtgtaccga aatcatgata tgttctacca ccgtgatttt caaaacctac
     1081 aatagttcca aaagtatcac tttcgataac aatatctcct gttaatcggt ttgtctttga
     1141 ttcagtataa aaatctaaaa tacctaaccc ttctaattct gtaccatcag gcgtgatata
     1201 ttttttccct aaaaattgat agcctccaca aatcgttaat cccggcatac catcttcaat
     1261 cgcttcttta agtggtgtct taattttact taattctttt gttgctaatg cttgttctct
     1321 atcacttcca ccaccgataa agaaaatatc acattcatca aaggtaatac cttctgtttc
     1381 attgatttct acgacattaa ctttaatatt tcgtttttta gcacgttgtc ttaaagcaat
     1441 aatatttcct atatcactgt ataaattcaa tttatctgac ataaaatgat aaatagtcaa
     1501 ttcatgcata ttatgattga cctccttcaa acgaacggtt taattgttca agcataggcg
     1561 ctaatgatgt atagtttggt attgcaactg tgaaaccttt ataatccata gtctttgccg
     1621 ttgctttata aatatcacgc tcaactataa ttggtacttc aacctctgct aacttcaatc
     1681 gcaattgaag ttcttctgct cgtgtacctg tcacgatgat agcttcaatt tgttgcttag
     1741 ataatttttc aaaatctgca tcataaatcc atgaagtatc tcgaccatct gcagcgttat
     1801 catttagcga aataacatac actttttcgc cttctaattg ttcaccaact gataaacttg
     1861 cattcattcc tgcaggattt ttagctaaat tgatcatcgc ttcttttcgt tcttttttaa
     1921 agtactgcat acgaccattg tctgatgtat acgtttcaaa gccattttta attgtttgtt
     1981 catttaaccc tagctctctt aaaacagtat atgctgctaa cgcgttataa gcgttaaagt
     2041 cacctgcaat tttcatatca tatttttcat cattgatatt taaatataaa aacggtgcca
     2101 catcaaaact tgatatttca tattttgctt gctctcgttt gaaaccacac tgacagtgat
     2161 aatgaccaat ttgattataa tgaatataat cgtattgcaa taagcgacca cagtttggac
     2221 aatatctact ttcattcatc gtactttgtt caaattcatg ggcatgtgct ttcataccat
     2281 agtacacaat cgtatcactt gcgattttca aacgactcac aaatggatca tcagcattta
     2341 gcaataattt gatgccttta ttactaattg tctctgcaat gttattaacc ataatatcaa
     2401 tttcaccgaa gcgatccatt tgatctctaa agaaattagt aaataccatc attgaaggtg
     2461 taacttcttt taacacacgt ggaatcgaac cttcatcaat ttcgattacc gcaattttag
     2521 tcttaggtgt tgattgcatg atgaatgcag aagttatacc tgcagccata ttagcacctt
     2581 cattattgtg tataatttga atattatttg cttttaaagt atgtccaatt aagtttgaag
     2641 tcgttgtttt accatttgtt ccactgataa atacaatatc atcaacttgc tctgctaatt
     2701 ttcttaatat atctgtatcc acttttctag cgatttgtcc aggtaaatct gttcctcttt
     2761 tacctactgc tctacttgct ttacgcgcca atttcgctag atggattgcc gtccactgtc
     2821 tcatgtgttt cctcctcaaa tttccactcg catcattata acatgacaag gcaacttcaa
     2881 aaaagtttct caatcacaaa ttgatacagt gtttctaaat tataatcatt ctcatttaga
     2941 taattaaaaa tgatatttat tctcaattaa atatcgctaa atgatacatt ttctcaatta
     3001 gagtgctaaa ataaggcatt aaattataat tattattatt tacaattgag aatgagtttg
     3061 ttatactaag tttaattcat agtaatttta atttacaaat aagaggtgta tcaaaatgtt
     3121 aagtaaaaat ttattagaag cattaaatga tcaaatgaac catgagtact ttgcagcaca
     3181 cgcatatatg gcaatggcag catactgtga taaagaatcg tacgaaggat ttgcaaactt
     3241 cttcattcaa caagctaaag aagaacgttt ccatggacaa aagatttata actatattaa
     3301 cgacagaggt gcacatgcag aattcagagc agtttcagca ccaaaaattg acttttcaag
     3361 catactagaa actttcaaag acagcttatc tcaagaacaa gaagtaacaa gacgtttcta
     3421 taacttatct gaaatcgctc gtcaagataa agattatgca actatctcat tcttaaactg
     3481 gttcttagat gaacaagtcg aagaagaatc aatgtttgaa actcacatca attatttaac
     3541 tcgtatcggc gatgacagca atgcattata tctttacgaa aaagaacttg gcgctcgtac
     3601 attcgacgaa gaataattaa acatcactac aatagacaga taaatatcat acgacatgat
     3661 aggcatttgg gtcacttaca ataacccaat gtctatatta ttttgcttta cggagatcac
     3721 tagattcatt ttctgaatca ttgatctgcg ttttttcatt ttcaaggcta attattgtat
     3781 ttttagtcat ttatttttta aactactaat gttaataact ctaaatttga tgttgaatta
     3841 atttgacgat tttaaagcat atcatcattt actttttaat cagagttaca tccaaatgat
     3901 agatttcacg ttataccttc acgtataata ttatgtatcg tttgtaagca aatgactaaa
     3961 agtctattaa tatatacatt taattaattg aaaggattga ctacatgata caagatgcgt
     4021 ttgttgcact tgattttgaa acagcaaatg gtaaacgtac aagtatttgt tctgtcggaa
     4081 tggttaaagt cattgatagt caaataacag aaacatttca tactcttgtg aatccgcaag
     4141 actatttttc acaacaaaat attaaaattc atggcataca accagaagat gttgaaaatg
     4201 cacctacgtt tgactacgta tttccatata tgatgcaatt tattgcagat ttacctgttg
     4261 tcgcacataa cgcggcattt gatatgaacg tcttacatca aagcattcaa aatattggtt
     4321 taccaactcc aaatttaact tacttttgta gttatcaact tgctaaaaga accgttgatt
     4381 cgtatcgata cggtttaaaa catatgatgg agttttatca attagatttt catggtcatc
     4441 atgatgcatt gaatgatgcc aaagcatgcg caatgattac ttttaggcta ctgaaaaatt
     4501 atgaaaattt aacatatgta actaatattt atggtaaaaa tctaaaagat aaaggctagg
     4561 actaaataaa atactccctt caaaagtaag cattgtaaaa atgtaaactt tgcagggagc
     4621 tttattttat ataaagtcat atatcgtcat atttttataa gttgattgtt ctaaattacc
     4681 tacagtgaca ccaataagtc gaattggtac atcagggtct tttaaatcgt tataaagtaa
     4741 atatgcaata ttataaatat cttcttcaga actaaccgaa tctcttaaac tcatctgttt
     4801 agatagcgtt tcaaattgat aagttttaat tttaaccgtt acagttttag ctgacttctg
     4861 taatttattt agacgttcag ctgttttacc tgacaattcc catacttttc ttaaaatctc
     4921 ttcatcatca ttcacgtctg ttgcaaatgt gcgttcagtc cctactgatt ttcttactct
     4981 tgatgatttc acttcactat ggtcaatacc gcgtgccttg ttatataaac cccgacctct
     5041 ttttccaaac aaacgtatta attcaaattc cgttttctca tataaatctc taccgttaaa
     5101 aataccatta tcatgcatta cttttttgga agctttacct acgcctggaa aatctccaat
     5161 atccaatgtc atcaaaatat catggacatt ttgataatca atcacagtca taccatcagg
     5221 tttattcata ccactcgcta atttagctaa aaatttgtta taagaaacac ctgcagatgc
     5281 tgttaaatgt gtctgctcta gaatatcttt tctaatatac tgagcaattt tcgaagcagg
     5341 aaggtctggt ctcactaatt ctgtaatatc taaatacgct tcatccaatg acatcggttc
     5401 taccttatct gtataacttc ggaaaataga cataatctgc gcagatgttt ctcggtaagc
     5461 accaaaatta cttgtgacaa agtatccatt tggacataat ttatgcgctt gtgacatagg
     5521 cattgctgaa tggacgccgt attttcgtgc ttcataggat gccgtagaga caacacccct
     5581 actgcttgct ttaccaccaa caatgactgg tttccctttc aatttggggt tatctctcat
     5641 ttcgacttgt gcaaaaaaat agtccatatc tatatgaata attcgtctct cagtcaagtg
     5701 ctcacctccc tactaatttt tacttttata acgcacaaaa atatctcaac ataattatac
     5761 gctgtgtacg atttttttac ataaatcttg cacttagcga taactatatt gagataacta
     5821 caagttgtta taaaatcaat tgctatttaa gcatgatgat gaagacgatt gagtaagaaa
     5881 acataggtaa tctgaaataa ttcaagcaaa ttcattttgt tggtatcatc atattaaaat
     5941 ttattattga gtcggctttt gatgatacaa ataaatacta tcttcatctt gttgaacata
     6001 tttaaaacta ctagatattg ttaatgcaac aagcagaata tctataaagc aataagcggt
     6061 atgcatacta aacataaaaa taagtgatga ataaccaaat accttaatta aaataagcaa
     6121 gccagtactt aataggatta gtggtgacag cataataatt gagaattgcc atttgttgaa
     6181 gcaagcatct gctgtttgga ataagattct gtcttttttt atattaaaca taggtttgct
     6241 atctttttta aataaaagaa ataatgctct atggataagt tcatgtaaaa tcaataaaat
     6301 aatgaatcca gcaaacccat atacaagatt gatgatgata ttttgatcga caaccgctgt
     6361 gacacctaac gcccacttat acgtaaataa aatcacgaat aacgcaataa caagttgcaa
     6421 gataataaac cttcgcattt gaaaattatt tgtcgttaaa tcaattttat gcattaccaa
     6481 ccctcccgat catgacattc ttattcttct ttaaatatag tatacaatgt cacatttaat
     6541 ttaaaaagtt catatcaaga aagtaaattg gctgtaataa aattttaata tacgacttct
     6601 ttcttcactt attaaggcga aattttatct caaatcatgt gcgctatttc aaattgaata
     6661 atgccactgt ctcaacatgt gttgtttgtg gaaacatatc taccggtgtt acctcttcaa
     6721 gttgatattt ttcagctaat aataatgcat cacgttgctg tgttgcggga ttacatgaaa
     6781 tatagacaat acgcttaggt tctaatgtaa gcaaagtctg aataaacgtt tcgtcacagc
     6841 cctttcttgg cggatcaacc attacaacat ctggtttaat cccttgtgct ttccattgta
     6901 aaataacttc ttcagctttc ccacagacaa aagttgtatt attgcattgg tttatagtcg
     6961 cattttgttg tgcgtcttca attgcagaag gtactacttc aacaccgtat acatgttttg
     7021 caagtggtgc catatatagc cctattgttc caataccaca ataggtatct aatacaactt
     7081 cattacctgt caattgcgca tactcaattg ctttattata taatttctct gtttgttcag
     7141 aattaatttg gtagaatgac tgatcactta ttttaaatgt actatctgtt aattgatcaa
     7201 taattgtatc tttaccatat agcgttatag attgacgtcc cataataaca ttagagtggc
     7261 tatcattaat gttttgttta atgcttgtca cattaggaaa tgcatctaat atcttctcaa
     7321 caacagcatt tttttgtggc cactttttac cattagttac aaaaataatc atcatttcgt
     7381 ctgtatgata tcctgttctt acaaccaaat gtctcattaa accttttttc aattgttctt
     7441 gataaatact tacatttaaa tcttttaaaa tagatttaac ttcattcatc acttcttgat
     7501 gttgtgaatc ttgtattaaa caactttcca tgtcaataat gtcatggctt ctttgacgat
     7561 aaaagcccat aataacttca ttctgttcat tcttaccaac tggaatctgg gacttgtttc
     7621 gatatctcca aggatctgtc atgccaactg tatcgttaat cttagaatta tcaaaatgcg
     7681 cttttcgctg aaacaaatta atcacttgtt cctttttcat ttcaagttgt gcttcgtatg
     7741 ataagtgttg aagttggcac ccaccacaac gttcataata tatacatggc ggtgcaacac
     7801 gattctcact tctcgtattt atattaagca acttacctat cgcaaagttc ttttttactt
     7861 tgataatttt atattcaatt tgttcattaa ttaaagcttg tggtatgaaa ataggaaagc
     7921 gatctatttt tacgacacca tggccttcat gcgttaaatc aacaactgtt cccgttttta
     7981 tgtcattttt agctattgct tgcaaaattt tacctccaaa atgaacaggt taggaacaaa
     8041 attatgcgct tcctaacctg ccattatata tttcactatt tctgtttatt cttcgattaa
     8101 attgtcatca acatgatcat tatttattaa ctcttcattt acaatatcat taggtgcaaa
     8161 gacatctata tgacgttcta gatttaagaa attcgctggt aatttaccac catattctcc
     8221 atctacattt agttgtaagt ctgtgaatga tgaaatatta attgcctttg ctttttcata
     8281 aataacttta ggatgcttag tatgttctcc tcttgaagct aaagtcataa tatgaccaag
     8341 ttctgcaagg tttgattttt caactataat taacgtaaaa tagccgtcat ctaacttagc
     8401 gtccggcact aatttttcaa atcctgccat tgaatttgtt aaacctaaaa agaataataa
     8461 tgcttctcct tggaaaacat taccatcata ttcaattctt aaatctacag ctttcatttg
     8521 aggtaacatt tcgaaacctt tgatgtaata agcaaatgga ccaacaatag atttcaattt
     8581 actcggtgtt tcataagaga cttgcgtcaa ttgtccgcct gcagctaaat taataaagta
     8641 tcgattattc attttaccaa tatctacttt agtagaatga ccttcaatga tgacatcaag
     8701 tgcccccatg atgtcattag gtatatgcaa tgcacgtcca aagtcattaa cagtacccat
     8761 aggaatgaca cctagcttag gacgattagg cttttctgcg ataccattaa ctacttcatt
     8821 taatgttcca tcaccacctg cagcgattaa tacatcataa ttttcatgca tagctctttc
     8881 tgcttcaagt gtggcatcac ctattttctc ggttgcatat gcactcgttt catatcccgc
     8941 tttttctaat tttattaagg catcaggtaa ttctctttta aatagctctt tacctgatgt
     9001 cgggttataa atgattctag cacgtttcct catatcttat ccctctactt aaaattcata
     9061 tattttaact tcatctttgt ttcgtctaat agggagtggg acagaaataa tatttaacaa
     9121 aatttatttc gttctacccc aacttgcatt gtctgtagaa tttcctttcg aaattctcta
     9181 tgttggggcc ccaccccaac ttgcacatta ttgtaagctg acagaaagtc agcttctttg
     9241 tttgggggcc ccgccaactt gcacattatt gtaagctgac agaaagtcag cttctatgtt
     9301 ggggccccac tagaattgaa aaaagcttgt tacaagcgta ttttctttca gtcaactaca
     9361 gccaatataa cattgtagtg cctaggacat tgaatttatg acccaggctc agtcttattt
     9421 catcattctt aatatcgtta aagaccaact tgtatcttaa acaaatacta tctcaatatg
     9481 tacaaagctt gttatttatt cagcattttt tgccgttctt cattatatag acttcgtcag
     9541 ttatgctatt ttacctttaa aatgatgttg taaatataat gttgtcttta tacaaaaatg
     9601 ttttatacct ttttattata cagtttgcct taatataatt aaagctaacg cattaataaa
     9661 attaatattt ttaccattaa catgtacaat gaataaagtt aaaagtaatt tgacttctat
     9721 agatataaat aaaccctcga ttgcatctaa gtcagcaatc aagggtttat tttttaaatc
     9781 ttcatagttt gatgatttaa attatctttt atctaattct tgttttaata gttgatttac
     9841 taattgtgga ttagcttgac ctttagacgc tttcataatt tgaccaacta agaagcccat
     9901 agctttgcct ttaccatttt tgtaatcttc aactgattgt tcgttattgt ctaatgcttc
     9961 atttacaaat tttagaagtg ttgcttcatc agaaatttga actaagccat tatcttccat
    10021 aatctgttta gcattaccac ctttagctgc taactctggg aagactttct tcgcaatttt
    10081 actgctcatt gttccgtctt cgataagttt aatcatacct gctaaatttt ctggtgttaa
    10141 tttagtatct aataattcta cttgattttt atttaaatat tcgtttacgc cacccattaa
    10201 ccagttagat gttaatttaa catctgcacc gtgttcaatt gttgattcaa agaaatctga
    10261 catttcttta gtcaatgtta atacgtgtgc atcgtatgca ggtaaaccta attcatttac
    10321 atacttagct ttacgttcat ctggtaattc aggaattgtc tgacgaacac gctctttcca
    10381 agcatcatca atatataaag gtacaatgtc aggctctggg aagtaacggt aatcatcaga
    10441 accttcttta acacgcatta aaattgtttt acctgtagat tcatcaaatc gacgtgtttc
    10501 ttgtccgatt tctccaccat ttaacaattc ttcttcttgg cgtttttctt catattctaa
    10561 acctttacgt acatagttaa atgagtttaa gtttttcaat tcggctttag taccaaattt
    10621 ttcttgacca tatggacgta aagagatgtt agcatcacaa cgtaaagatc cctcttccat
    10681 cttaacgtct gatacaccag tgtattgaat aattgaacgc aatttttcta aatatgcata
    10741 tgcttcttta ggtgaacgaa tatctggttc agatacgatt tcaattagcg gtgtaccttg
    10801 acggttcaag tcaactaatg aatactcacc tttatgtgtt gacttaccag catcttcttc
    10861 catgtgaaga cgagtaatac cgattcgttt tgtttcaccg tcgacttcga tatcgatata
    10921 tccattttca ccaattggtt gatcaaattg agaaatttga tatgcttttg gattatctgg
    10981 atagaaatag ttcttacggt caaacttaga ttctgttgcg atttccatat ttagtgccat
    11041 tgcagcacgc attgcccagt ctactgcacg cttattaaca actggtaaga cacctggata
    11101 tgctaagtcg ataacatttg tatttgagtt aggttctgct ccaaaatgcg ctggtgatgg
    11161 agaaaacatt tttgagtccg tttttaactc tacgtgaact tcaagtccta taactgtttc
    11221 aaaatgcatg atttccactc cttataattt ttcataaacg tcatgtaaat tgtattgtgt
    11281 ttcatattga taagcgacac gatataacgt tttttcatcg aatggtttac caatgaactg
    11341 taaaccgatt ggtcggccat ttgattgtcc acaaggaaca gaaataccag gtaatccagc
    11401 taagtttact ggtgttgtta ataaatcatt ggcatacatt gttaatggat catcaatttc
    11461 ttcacctaaa ttaaacgcag ttgtaggcgc tgttggacca actactacat cataattttc
    11521 gaatacttta tcaaagtcat ttttaatcaa tgttctaact ttttgagatt ttttatagta
    11581 agcatcatag taacctgaac ttaatgcaaa tgtacctaag aaaatacgac gttttacttc
    11641 tttaccgaaa ccttcagatc ttgacatttt atataattct tctaatgaat gagcttcttt
    11701 agaatgataa ccataacgaa ttccgtcaaa acgagaaagg tttgacgaag cttctgatga
    11761 tgcaatcacg taatatgatg gaataccaaa tttagtattt ggcaatgata cttcctcaac
    11821 gacagcacct aaagatttta aagtttctac agcgttttga actgcttctt ttacgtcatc
    11881 agctacacct tcacctaagt attctttagg taatgcaact tttaatcctt taatatcttt
    11941 accaatttca gatgtaaagt ctacatcatc aactggtgca cttgtagagt cattaacatc
    12001 tgcaccagaa atagcttcta atacgattgc attatctttt acatttcgag tcaatggacc
    12061 aatttggtct aatgaagatg caaaagcaac taatccaaat cgagatacac gaccgtatgt
    12121 tggtttcata ccgacaacgc cacaatatgc agccggttgt ctaattgaac cacctgtgtc
    12181 tgaacctaag ctaaatggta ctaagccagc tgcaactgct gctgcagatc cacctgatga
    12241 accacctggc actgctttat ggtcaaatgg gttaactgtt tttttgaaat aagatgtttc
    12301 tgttgaacca cccattgcaa actcatccat atttaattta ccgattaaaa cggcattttc
    12361 attatgtagt ttttccatta cagtagattc gtaaattggc acaaaacctt ctaacatttt
    12421 acttgcacat gttgtttcta atccgtttgt aataatgtta tcttttatac ccattggaat
    12481 accaaataat ttgccatcca tttgatcttt tgcttgtaat tcatccaatt cttgcgcttt
    12541 tttgattgca ttttctttat ccagcgctag aaaagactta attgttggat cagtctcttc
    12601 aattgcatca tatatatctt taacaacatc agatggtttg atttttttgt cttttattaa
    12661 agttaataaa ttctcaaccg attcgtagcg aatgctcatc ttacgcgtcc tcctcattca
    12721 tgattgtagg cactttaaat tgtccatctt ctgtttcttt ggcatttttc aaagctaatt
    12781 cttgtggaat acctttaatt gctttatctt cacgtaaaac gttttgtaaa tctaaaacgt
    12841 gatatgtagg ttcaacgcct tctgtatcag cgctatcatt ttgttttgca aaatctaaaa
    12901 tgctttctaa tgtgttggcc atttcttccg tttcttcagg agaaatttga agtcttgcaa
    12961 gattcgcgat atgctcaact tcttcacgtg ttacttttgt cattaataaa agcctccttt
    13021 aagtcattca tcactaaatt gtatcaaatt tccaattaaa aatctaagta tttatgaggt
    13081 gctactttaa tttcatataa actgtataaa cattatcatt cgtttatcaa atcatttttt
    13141 atgaaaacaa cactctttta atattagaca acccaattca atattatgat tatgcaattt
    13201 taattataaa aatttgctca tatccaaaaa atacgaaagc gctttctata ttggtatgca
    13261 agtatttcaa aaagaataaa tttaattttc ctacttttct aaacatttat ctttatgtat
    13321 aatgttttca agtaactaaa ttataaatta aataaaggga gtgtttatca tgcttacaat
    13381 ggggacagca ttaagtcaac aagtagatgc caattggcaa acttatatta tgattgccgt
    13441 ctacttcttg atactaatcg ttattggctt ttacggttac aagcaagcaa ctggtaacct
    13501 aagcgagtac atgttaggtg gacgtagtat tggaccgtat attactgcat tatcagctgg
    13561 agcttcagat atgagtggat ggatgattat ggggctacct ggttctgtct atagcactgg
    13621 tctatcagct atgtggatta caatcggttt aacattaggt gcttatataa attactttgt
    13681 tgttgctcct agacttcgtg tttataccga attagctgga gatgcaatta cattaccaga
    13741 tttctttaaa aatcgtttaa acgataaaaa taatgtgtta aagattattt ctggattgat
    13801 tatcgtagta ttctttacat tatatacaca ttctggtttc gtatctggtg gtaaactatt
    13861 tgaaagtgct tttggattag attatcattt cggtttaata ttagttgctt tcattgtcat
    13921 tttctatact ttctttggtg gatatttagc tgtatcaatt acagatttct tccaaggtgt
    13981 cattatgtta attgcgatgg ttatggtccc tattgttgct atgatgaatt taaacggctg
    14041 gggaacgttt catgatgtag cagctatgaa acctacaaat ttaaatttat ttaaagggtt
    14101 atcatttata ggaattatct ctctattttc atggggatta ggttatttcg gtcaacctca
    14161 tatcattata aggtttatgt ctattaaatc acacaagatg ctacctaaag ctagacgttt
    14221 aggtattagc tggatggctg ttggtttatt aggcgctgtg gctgttggtt taacaggtat
    14281 tgcattcgta cctgcttatc atattaaact agaagatcct gagacattat tcatcgtgat
    14341 gagtcaagta ctcttccatc ctcttgtagg tggtttctta cttgctgcga ttctagctgc
    14401 aattatgagc acgatttctt cacaattact tgtaacatct agttcactaa cggaagactt
    14461 ttataaatta attcgtggtg aagaaaaagc taaaacgcac caaaaagaat ttgttatgat
    14521 tggaagatta tctgtattag ttgtagcaat tgttgccatc gcgattgcat ggaatccaaa
    14581 cgacacaatt ctaaacttag taggtaacgc ttgggccgga tttggtgcat cgttcagtcc
    14641 acttgtgcta tttgcacttt actggaaagg tttgacacgt gccggtgctg taagtggaat
    14701 ggtttcaggt gccttagtcg ttatcgtttg gattgcgtgg attaaaccat tggcacatat
    14761 caacgaaata ttcggcttat atgaaattat tcctggattt attgtaagtg taatcgttac
    14821 atatgttgta agtaaactta ctaaaaaacc tggtgcattt gttgaaactg acttaaacaa
    14881 agttcgtgac atcgttagag aaaaataatt cataagtctt aacaaattaa aaaggtacta
    14941 atgttaatca aaattatgac taacattggt acctttttat tatctttaat ttacaattaa
    15001 ttactgtaaa tatgaacttg cggttctttg tcatcttttg tcttactaat taaagcacgt
    15061 ggttggttac catctttaat acgaatttca tagttatcga ttttatcgaa atatttattc
    15121 gcttgttcag taacgtactg tgtaatacca attgtttcag cttgtccata gtaatcgatt
    15181 ggtaaatcta ctactaatcg ttgtggcttt ttatcaacaa atttaacttt ccctactgct
    15241 tgtgtgaaat tagaaaaata tgattgcaaa ttatcattaa attgcttgaa attattattt
    15301 aaattttcat cataatctgc tgctgttgaa gaaggtaata aagctgattt ttcattgata
    15361 ttatgccatt cattaagctt tgtttgactc ttttctgcag tcgcttgagt gataaattca
    15421 cctggtgtga ttgaatcttc acttgattgc ttataaattg caaaatgaat tggtatatct
    15481 tttaaatcat cattttcacg taaccttgat aatatctcac tagccatttg tttaccttgc
    15541 tttttaactt cgctatcatc tagtttttta ctaaaagtcg atccatcttt ttctttttta
    15601 tagtaataaa cactattcat agctaaacca atcgtcatac ctttaatatt cttacctttt
    15661 gtatctccac caccataaaa atcttgctct aaaatgttag ataaataggc tggtgatttt
    15721 tctgcaatct tttcaggatc tgtttcacct tcgtgtgatg gattaagtcc taaattttca
    15781 ttcgctttct tgtctttttt atctttttca gacattttat cgatttcacg ttttgtatac
    15841 ttaggattta aataggcatt aattgttttc ttgtccaaaa attgaccatc ttgatacaaa
    15901 tatttatctg ttggaaatac ttctttactt aagttcaata aaccatcttc aaagtcgccg
    15961 ccattataac tatttgccat gttatcttgt aaaagtcctc ttgcctggct ttctttaaat
    16021 ggtaacaatg tacgatagtt atcaccttgt acatttttat ccgttgcaat ttcttttact
    16081 tgatttgaac tattgttatg tttttgatta tcttttccag cctggtcatc cttatggtta
    16141 ccacaagcag cgagtataaa gatagctgta atcaataata ctaatgtacg cttcatcgac
    16201 atacccctct aactatttaa ttcattttgc ttatctacaa attgttgctc tgtccaaatt
    16261 tcaataccta aactttgtgc ttttgttaat tttgaacctg catcttcacc agcaataacg
    16321 acatctgtat ttttagtaac gctacttgta actttagcac cttgtgatgc aagccattta
    16381 gatgcttcat tgcgtgtcat ttgatgtagc ttaccagtca gtactatcgt tttaccacta
    16441 aattcaggat gtccttcaat atctgatgtt ttgatacctt tataaatcat attaacatgt
    16501 ttatctttta atttttgaat taaagcacga atatcttcat tttctaaata agtaactaca
    16561 gattgtgcta ctttatcacc tatatcatga atttctacta attccgcttc agttaccgtt
    16621 agtaatcgat ctatcgtttc atatttttct gctaacactt ggctcgcttt aacacctaaa
    16681 tgcctaatac ctagaccaaa taataaattt tctaaagagt tgtccttagc ttgttgaatg
    16741 gcagctaata aattatcaac ttttttctgc cccattctgt ctaaaggtaa taaatcttct
    16801 tctgttaaat agaaaatatc agcaacatct ttaattaatt cgctttgata aagctgttga
    16861 ataattttag tgcctaaacc atcaatattc atggcttgtc ttgatacaaa gtgaatcaat
    16921 ccttcaacaa gttgtgcttg gcattttgga ttaatgcaac gaagtgctac ttcgccttca
    16981 atacgtacta attcatgtcc acaacttgga caatgggttg gcatatgata tgtgacagca
    17041 tcctcaggtc tacgttctgg aatactacgt acaacttcag gtatgatgtc acctgctttt
    17101 ttcactacaa cactatcacc aattcgaata tctctgtcat gaattaaatc ctcattgtgc
    17161 aaagatgcac ttgatacagt tgtaccagct acttttactg gttctaaaat agcagtaggt
    17221 gtgactacac ctgttcgtcc aatacttaat tcaatatcta ataatttagt tactacttcc
    17281 tcagctggaa atttataagc aatggcccat ctaggagatt tttgtgtgaa tcccatctca
    17341 tcctgttgat ctaaatcatt aaccttaata acaatcccat caatatcata aggtaatgac
    17401 tctctttggc ttgtccattt ttcaatatac tctaaaacac catcgatatt atttacacgc
    17461 gctctatttt tattcgttgt aaaacctaat ttatctaact catctaatgc ttcactttgc
    17521 gaacgcgcat tgaaatcagt gaaatcattg acactatata taaatacgct tagctttcgt
    17581 tttgccgtta atttagaatc taactgtctt aatgatcccg cagcagcgtt tcttggattt
    17641 gcaaataact gctcatcatt tttttctttt tcttcattta atcgtaaaaa tgaacgtctc
    17701 ggcatatatg cttcaccacg aacttctaca tttaatggtt ctttcatttt caaaggtatc
    17761 gcatgaattg tttttaaatt ttcggtaata tcttcacctg ttgttccatc accacgtgtt
    17821 aaaccttgaa cgaagtatcc atcaacatat ttcaatgata ctgctaagcc atcaattttt
    17881 aattcgcaca tatattcaac gttgccaatt tgttcacgta tgcgttggtc gaattttctc
    17941 aaatcatcct cattaaatgc attccctaaa cttaacattg gcgtgtcatg gttgacttta
    18001 ttgaaagagg cttgggcttc accgccaact ctaactgttg gagaatctac agtcttatac
    18061 tcaggatgct cctcttctat tttaatcagt tcatgaagta atttgtcata ttcactatct
    18121 ggtacagatg gattatcctc tacatagtat tcataactgt attgatttaa taaatcatgt
    18181 aactcgttca cacgagacga taaatcagcc atcccttaat cctccttttt ttcaattggt
    18241 gcaaattgcg ctaacaaacg ttttggccct tgtgatttaa agataatatc tagttcgatt
    18301 gagccatttt tctcgtttac attactcacc atgccttctc cccaggcttt atgcatcact
    18361 ttgtcaccta cattccaatc agatgacaat acttgttttt tcgttgacgt tgttcgttga
    18421 ctaaatccgc gtttagcaaa aggttttgcc ttaggttgta tcgtttgtcg tttgccactt
    18481 gaatgatttt ctaatagtga ttctggaatt tcctttaaaa atctggatgg catatttgac
    18541 tgagggcgac caaataacat tcttgatgtc gcatgagtga tatataacac ctcttcagcc
    18601 cttgtaattg ctacataaca aatacgacgt tcttcttgca tttcatgatc atcttcactc
    18661 ttaatcgctc taatatgtgg gaataaagat tcttccatcc ccattataaa gacaattgga
    18721 aattcaaggc ccttagccga gtgcatcgtc attagtgtta cgccattttc agtatctgcc
    18781 tcatcaatat cagctactaa cgataaatcc gttaaaaagt taattaatga ctgttcttct
    18841 aatggggtat tttcctcata gtcttttggt actgacataa attcatcgat gttttctaat
    18901 ctacttcgag attctaatgt attttcacgt tcaagcattt ctcgatagcc agacttttgt
    18961 aatacttcat caactatttc atgaatttct aaaaattctt gttcttttat caagctttgg
    19021 attaactcgt aaaaattaag acactcttgt gtcacctttt ttgacaatcc gataaaatca
    19081 gcttctccaa gtgcatcaaa catactgata ttgttttgaa gtgcatagtt ttgaactttt
    19141 tcaacagatg aaggacctac acctcttttt ggaacattaa taatacgttg caaactaatg
    19201 tcatcattac tattggcaat tatacgcaaa taacttaata aatctttgat ttctttacgg
    19261 tcatagaact tttggccacc aaccattgtg tatggcatat tagatttcat gaatgtttcc
    19321 tcaagtacac gtgattgtgc attcgttcta tataaaattg ccatatcttg atatttctta
    19381 ccattacgtt gatgcttcat aatttctcgt attacaaatt ccgcttcatc acgttccgtc
    19441 attgcttcat agtaatgaat tttctcacca ttcgtatttg cagtccacag tccttttggc
    19501 ttacgttcag aattattttt aatcacttcg ttagccgcat ttaaaatcgt tttcgtcgaa
    19561 cgataatttt gctctaaaaa gattgtattc gcttctggat agtctttttc aaatgataag
    19621 atattttgaa tatcagcacc acgccaacca taaattgact gatctgagtc acctacaaca
    19681 cataagtttt taaacttact tgctaataat ttaactaatg tgtattgtgc tttattagta
    19741 tcttgatact catctacatg aatatattgg aatttgttct gataatattc tagaacttct
    19801 ggtacacgct caaataagtt aatcgttgtc ataataaggt catcaaaatc taacgcttca
    19861 ttacgtgaca attggcgttg atatccacta taaaccgttg ctaccatttg cgagtgataa
    19921 tctgtggctt ctttttgagc atctgcaggt gttttaagtt catttttcaa attactgatc
    19981 gcaccgataa acatacgagg ttcaaacttt ttactatcaa tattttcatt ttttaagacg
    20041 tctttaataa cagatttttg gtctgtagga tcaattatcg taaaattgcg ttctatacca
    20101 attcgatctg catcacgacg taaaatacga acacacattg agtggaatgt tgacatccaa
    20161 ataacttctg cttgatcacc tactaatttt tgaacacgtt ctttcatttc tcttgcagct
    20221 ttatttgtaa aagtaatagc caaaacattg tatggtgaga catctttttc gtctaataaa
    20281 taagcaattc tatgtgttaa aacacgtgtc ttccctgaac cagcacctgc cataattaac
    20341 aatggtcctt ctgttgtctt tacagcttca ctttgctctg tattcatatg atttaataac
    20401 gcattcattt actagactcc tttattttta ctgtttttaa agctttttta atatctttat
    20461 aaataatatc accgacgata attgtatctg caatagctgc catctctgtc gcttgttgtt
    20521 ctgaggaaat accgccacca taaaaaagtt gcgtttctgt tagatgttct gagacagctt
    20581 gaacctttga tacgtcgcca taaatgccac tatactctat atacataacc ggtaatcgat
    20641 acatatgatt gaccatttgg gcatatgctt ctaaatcttc tgttgttaaa tctgtatttg
    20701 ctttggtatg ttttgccatt ttgctatcag cattgcacac gacataccct tcaaatatta
    20761 cttcctcaaa atctatacta tgtccatatg ttttaagcgc ttctaataat gtaccattgt
    20821 gaaatacaac atctgtactg ttcagtactg taggtacaaa ataaaaatca aaaccaggca
    20881 ttacactttc gatgtttgat atttcaagta ctaaaggcaa tgggtatctt cttactctgc
    20941 tcattaaatg aatgacatta tcttcagtaa cgtcatcagt tccaccaatc ataattgcat
    21001 ctgtttgaga catacaaatc gcatctaaat catcatctga aatatgttta gctgggtcta
    21061 atttaaaaat atggcgccat tttttaatgt catacatttt ccgaaactcc ttttttaaca
    21121 tacatatgat tatagcattt ttaagtgtcg agttctaaag acttaatatg aaatttaagt
    21181 tcttttactc tgtaaatttt ttcgtgaaaa aacaatgatt atctacctta ttagtgcaga
    21241 tagataacca ttgtttatcg ttcatttatt attcatttgt ttcaatattc atacgatcta
    21301 aaatcattgt ataagcatca ttaccccatt gtaatgaacg cttcactcta gaaatcgttg
    21361 cagtcgatgc tccagattct tgttcaatcg ttgcataggt ataaccttgc ttaatcattt
    21421 tagcaacttg taatctttga gacagtgatt gaatttcatt tacagtacac aaatcatcga
    21481 aaaattggta acattcttct ctattttcta acgttagtat tgcatcaaac aattcatcta
    21541 acgctgcacc tcgtaatttt tcaatttgca tatcgaaaca acccctataa ttttattctt
    21601 tatgtcattt taacgcatta atctattaaa gtgtagcgat ttatatttta ttaaatctga
    21661 atcgttactt tatttaattt tatgctaatc cagcgcgttc gaaaatagtg tcaacttgat
    21721 tcaaatgatg tttaggatcg aaacattcat ccaattcttc ttttgttaaa acacttgtaa
    21781 tagactcatc ttgttcgatt aattcacgga acggtgtttt cgtttcccaa gatatcatcg
    21841 cttttggttg tactttgtcg tatgcttctt cacgaaccat acctttatta attaatgcta
    21901 ataagacacg ttgtgagaaa atcagaccaa atgttttatc tatgttatta cgcatattat
    21961 cttcaaatac agttaaacgg tccacaatat ttgtgaaacg attcaatgca taatctagtg
    22021 ctattgtaac atctggtaac ataatacgct cagcagaaga atgagaaata tctctttcat
    22081 gccataatgg cacattctca taagctgtag taatataacc acgaatgact cttgaaatac
    22141 ctgtgatatt ttcagaacca attggatttc gtttatgagg cattgcagat gaaccttttt
    22201 ggccttttgc aaatgcttct tcaacttctc tcgtttcggt tttttgaagg ttacgtattt
    22261 caacggcaaa tttttctagt gatgtcgcga ttaatgctaa tgtcgcaata tagtatgcat
    22321 gtcgatcgcg ttgcaatgtt tgcgttgata caggcgctgt gccaatacct aaatgtttac
    22381 acacataact ttctatttca ggaggaatgt tagcaaaagt acctactgca ccactcattt
    22441 tccctacttc aatttcttct cttacttgtt tgaaacgttg taagttacgt tgcatttccg
    22501 tgtaccacaa tgccattttg acaccaaatg tagttggttc tgcatgcact ccatgtgtac
    22561 gtcccatcat caatgtatat ttataatttt ttgctttttc agctaaaacg tcgataaatc
    22621 tttctaaatc tttttcaata atgtcatttg cttgtttaat aacgaaactt aaagctgtat
    22681 ctacaacatc agtagaagtt aaaccataat gtacccactt acgttcttca cctagcgttt
    22741 cagaaacttg tctagtaaag gctacaacat catggcgcgt ttcttgttca atttcttgtg
    22801 cacgttcgac atttaccttt gcgttttgac gaattttttg tacgtcagct ttcggtatat
    22861 gtcctaattc actccatgct tcacatgcta aaatttccac ttctaaccat gcttcatagc
    22921 gattttgatc agtccaaata ttagacattt cttctctaga atagcgttca atcatttttt
    22981 taactcctat cgtatgtata ctcgaatttt caaaaacaga acatttttta ataaaaataa
    23041 acaaaaagtt cgtttttaac tctactctt
//
LOCUS       DALAGD010000042        23042 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.042, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000042 DALAGD010000000
VERSION     DALAGD010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 23042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23042
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.042"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(219..1217)
                     /gene="sirC"
                     /locus_tag="O2701_001311"
     CDS             complement(219..1217)
                     /gene="sirC"
                     /locus_tag="O2701_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirC"
                     /protein_id="HCZ4293214.1"
                     /translation="MTEKINKKDNYHLIFALIFLAIVSVVSMMIGSSFIPLQRVLMYF
                     INPNDSMDQFTLEVLRLPRITLAILAGAALGMSGLMLQNVLKNPIASPDIIGITGGAS
                     LSAVVFIAFFSHLTIHLLPLFAVLGGAVAMMILLVFQTKGQIRPTTLIIIGISMQTLF
                     IALVQGLLITTKQLSAAKAYTWLVGSLYGATFKDTIILGMVILAVVPLLFLVIPKMKI
                     SILDDPVAIGLGLHVQRMKLIQLITSTILVSMAISLVGNIGFVGLIAPHIAKTIVRGS
                     YAKKLLMSAMIGAISIVIADLIGRTLFLPKEVPAGVFIAAFGAPFFIYLLLTVKKL"
     gene            complement(1214..2209)
                     /gene="sirB"
                     /locus_tag="O2701_001312"
     CDS             complement(1214..2209)
                     /gene="sirB"
                     /locus_tag="O2701_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirB"
                     /protein_id="HCZ4293215.1"
                     /translation="MLLKPKYQIVIAGLCLAIVAILSLMIGNTLVSPGTVIQALFNFD
                     SENDLHDVVTGARASRTIIALLTGAALAVSGLLMQALTRNPIASPGLFGVNAGAVFFV
                     IFSITFIQIQSFKMIVVIAFLGAIVVTVLVVALGMFRQTLFSPHRVILAGAAIAMLFT
                     AFTQGILIMNETDLQGLLFWLSGSVSLRNIWDIPWIIPLVLILILIAFSMAAHINILM
                     TSDDIATGLGQNIKLIKWMIIMLISMLAGISVAVAGSIVFVGLIVPNISKRLLPPNYK
                     YLIPFTALAGAILMIISDIVARIIIKPLELPIGVVTAVIGAIVLIYIMKKGRQRL"
     gene            complement(2225..3217)
                     /gene="sirA"
                     /locus_tag="O2701_001313"
     CDS             complement(2225..3217)
                     /gene="sirA"
                     /locus_tag="O2701_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter
                     substrate-binding protein SirA"
                     /protein_id="HCZ4293216.1"
                     /translation="MNKVIKMLVVTLAFLLVLAGCSGNSNKQSSDNKDKETTSIKHAM
                     GTTEIKGKPKRVVTLYQGATDVAVSLGVKPVGAVESWTQKPKFEYIKNDLKDTKIVGQ
                     EPAPNLEEISKLKPDLIVASKVRNEKVYDQLSKIAPTVSTDTVFKFKDTTKLMGKALG
                     KEKEAEDLLKKYDDKVAAFQKDAKAKYKDAWPLKASVVNFRADHTRIYAGGYAGEILN
                     DLGFKRNKDLQKQVDNGKDIIQLTSKESIPLMNADHIFVVKSDPNAKDAALVKKTESE
                     WTSSKEWKNLDAVKNNQVSDDLDEITWNLAGGYKSSLKLIDDLYEKLNIEKQSK"
     gene            3448..4428
                     /gene="sbnA"
                     /locus_tag="O2701_001314"
     CDS             3448..4428
                     /gene="sbnA"
                     /locus_tag="O2701_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diaminopropionate biosynthesis protein SbnA"
                     /protein_id="HCZ4293217.1"
                     /translation="MIEKSQACHDSLLDSVGQTPMVQLHQLFPKHEVFAKLEYMNPGG
                     SMKDRPAKYIIEHGIKHGLITENTHLIESTSGNLGIALAMIAKIKGLKLTCVVDPKIS
                     PTNLKIIKSYGANVEMVEEPDAHGGYLMTRIAKVQELLATIDDAYWINQYANELNWQS
                     HYHGAGTEIVETIKQPIDYFVAPVSTTGSIMGMSRKIKEVHPNAQIVAVDAKGSVIFG
                     DKPINRELPGIGASRVPEILNRSEINQVIHVDDYQSALGCRKLIDYEGIFAGGSTGSI
                     IAAIEQLITSIEEGATIVTILPDRGDRYLDLVYSDTWLEKMKSRQGVKSE"
     gene            4425..5435
                     /gene="sbnB"
                     /locus_tag="O2701_001315"
     CDS             4425..5435
                     /gene="sbnB"
                     /locus_tag="O2701_001315"
                     /EC_number="1.5.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate
                     dehydrogenase SbnB"
                     /protein_id="HCZ4293218.1"
                     /translation="MNREMLYLNRSDIEQAGGNHSQVYVDALTEALTAHAHNDFVQPL
                     KPYLRQDPENGHIADRIIAMPSHIGGEHAISGIKWIGSKHDNPSKRNMERASGVIILN
                     DPETNYPIAVMEASLISSMRTAAVSVIAAKHLAKKGFKDLTIIGCGLIGDKQLQSMLE
                     QFDHIERVFVYDQFSEACARFVDRWQQQRPEINFIATENAKEAVSNGEVVITCTVTDQ
                     PYIEYDWLQKGAFISNISIMDVHKEVFIKADKVVVDDWSQCNREKKTINQLVLEGKFS
                     KEALHAELGQLVTGDIPGREDDDEIILLNPMGMAIEDISSAYFIYQQAQQQNIGTTLN
                     LY"
     gene            5456..7210
                     /gene="sbnC"
                     /locus_tag="O2701_001316"
     CDS             5456..7210
                     /gene="sbnC"
                     /locus_tag="O2701_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis protein SbnC"
                     /protein_id="HCZ4293219.1"
                     /translation="MQNHTAVNTAQAIILRDLVDALLFEDIAGIVSNSEITKENGQTL
                     LIYERETQQIKIPVYFSALNMFRYESSQPITIEGRVSKQPLTAAEFWQTIANMNCDLS
                     HEWEVARVEEGLTTAATQLAKQLSELDLASHPFVMSEQFASLKDRPFHPLAKEKRGLR
                     EADYQVYQAELNQSFPLMVAAVKKTHMIHGDTANIDELENLTVPIKEQATDMLNDQGL
                     SIDDYVLFPVHPWQYQHILPNVFAKEISEKLVVLLPLKFGDYLSSSSMRSLIDIGAPY
                     NHVKVPFAMQSLGALRLTPTRYMKNGEQAEQLLRQLIEKDEALAKYVMVCDETAWWSY
                     MGQDNDIFKDQLGHLTVQLRKYPEVLAKNDTQQLVSMAALAANDRTLYQMICGKDNIS
                     KNDVMTLFEDIAQVFLKVTLSFMQYGALPELHGQNILLSFEDGRVQKCVLRDHDTVRI
                     YKPWLTAHQLSLPKYVVREDTPNTLINEDLETFFAYFQTLAVSVNLYAIIDAIQDLFG
                     VSEHELMSLLKQILKNEVATISWVTTDQLAVRHILFDKQTWPFKQILLPLLYQRDSGG
                     GSMPSGLTTVPNPMVTYD"
     gene            7203..8459
                     /gene="sbnD"
                     /locus_tag="O2701_001317"
     CDS             7203..8459
                     /gene="sbnD"
                     /locus_tag="O2701_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B export MFS transporter"
                     /protein_id="HCZ4293220.1"
                     /translation="MINQSIWRSNFRILWLSQFIAIAGLTVLVPLLPIYMASLQNLSV
                     VEIQLWSGIAIAAPAVTTMIASPIWGKLGDKISRKWMVLRALLGLAVCLFLMALCTTP
                     LQFVLVRLLQGLFGGVVDASSAFASAEAPAEDRGKVLGRLQSSVSAGSLVGPLIGGVT
                     ASILGFSALLMSIAVITFIVCIFGALKLIETTHMPKSQTPNINKGIRRSFQCLLCTQQ
                     TCRFIIVGVLANFAMYGMLTALSPLASSVNHTAIDDRSVIGFLQSAFWTASILSAPLW
                     GRFNDKSYVKSVYIFATIACGCSAILQGLATNIEFLMAARILQGLTYSALIQSVMFVV
                     VNACHQQLKGTFVGTTNSMLVVGQIIGSLSGAAITSYTTPATTFIVMGVVFAVSSLFL
                     ICSTITNQINDHTLMKLWELKQKSAK"
     gene            8449..10185
                     /gene="sbnE"
                     /locus_tag="O2701_001318"
     CDS             8449..10185
                     /gene="sbnE"
                     /locus_tag="O2701_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2,3-diaminopropanoate--citrate ligase SbnE"
                     /protein_id="HCZ4293221.1"
                     /translation="MQNKELIQHAAYAAIERILNEYFREENLYQVPPQNHQWSIQLSE
                     LETLTGEFRYWSAMGHHMYHPEVWLIDGKSKKITTYKEAIARILQHMAQSADNQTAVQ
                     QHMAQIMSDIDNSIHRTARYLQSNTIDYVEDRYIVSEQSLYLGHPFHPTPKSASGFSE
                     ADLEKYAPECHTSFQLHYLAVHQDVLLTRYVEGKEDQVEKVLYQLADIDISEIPKDFI
                     LLPTHPYQINVLRQHPQYMQYSEQGLIKDLGVSGDSVYPTSSVRTVFSKALNIYLKLP
                     IHVKITNFIRTNDLEQIERTIDAAQVIASVKDEVETPHFKLMFEEGYRALLPNPLGQT
                     VEPEMDLLTNSAMIVREGIPNYHADKDIHVLASLFETMPDSPMSKLSQVIEQSGLAPE
                     AWLECYLNRTLLPILKLFSNTGISLEAHVQNTLIELKDGIPDVCFVRDLEGICLSRTI
                     ATEKQLVPNVVAASSPVVYAHDEAWHRLKYYVVVNHLGHLVSTIGKATRNEVVLWQLV
                     AHRLMTWKKEYANNAVFVDCVEDLYQTPTIAAKANLMSKLNDCGANPIYTHIPNPICH
                     NKEVSYCESNNS"
     gene            10094..11944
                     /gene="sbnF"
                     /locus_tag="O2701_001319"
     CDS             10094..11944
                     /gene="sbnF"
                     /locus_tag="O2701_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(L-alanin-3-ylcarbamoyl)-2-[(2-
                     aminoethylcarbamoyl)methyl]-2-hydroxypropanoate synthase
                     SbnF"
                     /protein_id="HCZ4293222.1"
                     /translation="MIVVQTLFIHIYQIQFVITRRYRIVNQTILNRVKTRVMHQLVSS
                     LIYENIVVYKASYQDGVGHFTIEGHDSEYRFTAEKTHSFDRIRITSPIERVVGDEADT
                     TTDYTQLLREVVFTFPKNDEKLEQFIVELLQTELKDTQSMQYRESNPPATPETFNDYE
                     FYAMEGHQYHPSYKSRLGFTLSDNLKFGPDFVPNVKLQWLAIDKDKVETTVSRNVVVN
                     EMLRQQVGDKTYEHFVQQIEASGKHVNDVEMIPVHPWQFEHVIQVDLAEERLNGTVLW
                     LGESDELYHPQQSIRTMSPIDTTKYYLKVPISITNTSTKRVLAPHTIENAAQITDWLK
                     QIQQQDMYLKDELKTVFLGEVLGQSYLNTQLSPYKQTQVYGALGVIWRENIYHMLIDE
                     EDAIPFNALYASDKDGVPFIENWIKQYGSEAWTKQFLAVAIRPMIHMLYYHGIAFESH
                     AQNMMLIHENGWPTRIALKDFHDGVRFKREHLSEAASHLTLKPMPEAHKKVNSNSFIE
                     TDDERLVRDFLHDAFFFINIAEIILFIEKQYGIDEELQWQWVKGIIEAYQEAFPELNN
                     YQHFDLFEPTIQVEKLTTRRLLSDSELRIHHVTNPLGVGGINDATTISET"
     gene            11919..12695
                     /gene="sbnG"
                     /locus_tag="O2701_001320"
     CDS             11919..12695
                     /gene="sbnG"
                     /locus_tag="O2701_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis citrate synthase
                     SbnG"
                     /protein_id="HCZ4293223.1"
                     /translation="MQQLSLKHRLNNGDSVYGIFNSIPDPLMIEVIAASGYDFVVIDT
                     EHVAINDETLAHLIRAAEAAHIIPIVRVTAVIDRDIIKVLDMGARGIIVPHVKDRETV
                     EHIVKLSRYYPQGLRSLNGGRMARFGRTPLLDAMEMANEHIMVIAMIEDVEGVMAIDD
                     IAQVEGLDMIVEGAADLSQSLGIPWQTRDDQVTSHVQHIFEVVNAHGKHFCALPREDE
                     DIAKWQAQGVQTFILGDDRGKIYRHLSASLATSKQKGDEG"
     gene            12695..13897
                     /gene="sbnH"
                     /locus_tag="O2701_001321"
     CDS             12695..13897
                     /gene="sbnH"
                     /locus_tag="O2701_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis decarboxylase
                     SbnH"
                     /protein_id="HCZ4293224.1"
                     /translation="MRIVQPVIEQLKAQSHPVCHYIYDLVGLEHHLQHITSSLPSNCQ
                     MYYAMKANSERKILDTISQYVEGFEVASQGEIAKGLAFKPANHIIFGGPGKTDEELRY
                     AVSEGVQRIHVESMHELQRLNAILEDEDKTQHILLRVNLAGPFPNATLHMAGRPTQFG
                     ISEDEVDDVIEAALAMPKIHLDGFHFHSISNNLDSNLHVDVVKLYFKKAKAWSEKHRF
                     PLKHINLGGGIGVNYADLTNQFEWDNFVERFKTLIVEQEMEDVTLNFECGRFIVAHIG
                     YYVTEVLDIKKVHGAWYAILRGGTQQFRLPVSWQHNHPFDIYRYKDNPYSFEKVSISR
                     QDTTLVGQLCTPKDVFAREVQIDAISTGDVIVFKYAGAYGWSISHHDFLSHPHPEFIY
                     LTQTKEDE"
     gene            13901..14665
                     /gene="sbnI"
                     /locus_tag="O2701_001322"
     CDS             13901..14665
                     /gene="sbnI"
                     /locus_tag="O2701_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional
                     regulator/O-phospho-L-serine synthase SbnI"
                     /protein_id="HCZ4293225.1"
                     /translation="MNHIHEHLKLVPVDKIDLHETFEPLRLEKTKSSIEADDFIRHPI
                     LVTAMQHGRYMVIDGVHRYTSLKALGCKKVPVQEIHETQYSISTWQHKVPFGVWWETL
                     QQEHRLPWTTETRQEAPFITMCHGDTEQYLYTKDLGEAHFQVWEKVVASYSGCCSVER
                     IAQGTYPCLSQQDVLMKYQPLSYKEIEAVVHKGETVPAGVTRFNISGRCLNLQVPLAL
                     LKQDDDVEQLRNWKQFLADKFANMRCYTEKVYLVEQ"
     gene            14861..15268
                     /locus_tag="O2701_001323"
     CDS             14861..15268
                     /locus_tag="O2701_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293226.1"
                     /translation="MSSIIGKIAIWIGIVAQIYFSVVFVRMISINIAGGSDYETIFLL
                     GLILALFTVLPTIFTAIYMESYSVIGGALFIVYAIIALCLYNFLSSILWLIGGILLIW
                     NKYSKDESTDENEKVDIESTENQFESKDKITKE"
     gene            15318..15944
                     /locus_tag="O2701_001324"
     CDS             15318..15944
                     /locus_tag="O2701_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ4293227.1"
                     /translation="MEKNVEKSFIKIGLYFQIAYIVLMAITLCGFVICYGLIFGLFYL
                     LSGSRADYLIVTIVISAIISIFVIILSIVPVIVLASDLFKERISKGVILIVLAIIALV
                     LCNFVSAILWFVSAISILGRKKLVAAADTTTIQKSKGNANQASHKDTCKKELDSQDMM
                     EHPEVKNPTTKNLEGFNEEIHKDEATTKVVSDNTEPPIESKDHVSKKD"
     gene            16156..16932
                     /locus_tag="O2701_001325"
     CDS             16156..16932
                     /locus_tag="O2701_001325"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HCZ4293228.1"
                     /translation="MTNNKVALVTGGAQGIGFKIAERLVEDGFKVAVVDFNEEGAKAA
                     ALKLSSDGTKAIAIKADVSNRDDVFNAVRQTAAQFGDFHVMVNNAGLGPTTPIDTITE
                     EQFKTVYGVNVAGVLWGIQAAHEQFKKFNHGGKIINATSQAGVEGNPGLSLYCSTKFA
                     VRGLTQVAAQDLASEGITVNAFAPGIVQTPMMESIAVATAEEAGKPEAWGWEQFTSQI
                     ALGRVSQPEDVSNVVSFLAGKDSDYITGQTIIVDGGMRFR"
     gene            17076..17198
                     /locus_tag="O2701_001326"
     CDS             17076..17198
                     /locus_tag="O2701_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293229.1"
                     /translation="MHQLKALLVLTHPRYYKTSQKHHYLIYLNKNSQSYLILFL"
     gene            17268..18248
                     /locus_tag="O2701_001327"
     CDS             17268..18248
                     /locus_tag="O2701_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCZ4293230.1"
                     /translation="MEDLERVLITGGAGFIGSHLVDDLQQDYDVYVLDNYRTGKRENI
                     KSLADDHVFELDIREYDAVEQIMKTYQFDYVIHLAALVSVAESVEKPILSQEINVVAT
                     LRLLEIIKKYNNHIKRFIFASSAAVYGDLPDLPKSDQSLILPLSPYAIDKYYGERTTL
                     NYCSLYNIPTAVVKFFNVFGPRQDPKSQYSGVISKMFDSFEHNKPFTFFGDGLQTRDF
                     VYVYDVVQSVRLIMEHKDAIGHGYNIGTGTFTNLLEVYRIIGELYGKSVEHEFKEARK
                     GDIKHSYADISNLKALGFVPKYTVETGLKDYFNFEVDNIEEVTAKEVEMS"
     gene            18211..18903
                     /locus_tag="O2701_001328"
     CDS             18211..18903
                     /locus_tag="O2701_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCZ4293231.1"
                     /translation="MKKLQLKKWKCRENDIEAVHNNKGYAYQRKLDKLEEVRKSYYPI
                     KRAIDLILSIVLLFLTLPIMVIFAIAIVIDSPGNPIYSQVRVGKMGKLIKIYKLRSMC
                     KNAEKNGAQWADKDDDRITNVGKFIRKTRIDELPQLINVVKGEMSFIGPRPERPEFVE
                     LFSSEVIGFEQRCLVTPGLTGLAQIQGGYDLTPQQKLKYDMKYIHKGSLMMELYISIR
                     TLMVVITGEGSR"
     gene            19113..20279
                     /locus_tag="O2701_001329"
     CDS             19113..20279
                     /locus_tag="O2701_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCZ4293232.1"
                     /translation="MKIIYCITKADNGGAQTHLIQLANHFCVHNDVYVIVGNHGPMIE
                     QLDARVNVIIIEHLVGPIDFKQDILAVKVLAQLFSKIKPDVIHLHSSKAGTVGRIAKF
                     ISKSKDTRIVFTAHGWAFTEGVKPAKKFLYLVIEKLMSLITDSIICVSDFDKQLALKY
                     RFNRLKLTTIHNGIADVPAVKQTLKSQSHNNIGEVVGMLPNKQDLQINAPTKHQFVMI
                     ARFAYPKLPQNLIAAIEILKLHNSNHAHFTFIGDGPTLNDCQQQVVQAGLENEVTFLG
                     NVINASHLLSQYDTFILISKHEGLPISIIEAMATGLPVIASHVGGISELVADNGICMM
                     NNQPETIAKVLEKYLIDSDYIKMSNQSRKRYLECFTEEKMIKEVEDVYNGKSTQ"
     gene            20260..21498
                     /locus_tag="O2701_001330"
     CDS             20260..21498
                     /locus_tag="O2701_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCZ4293233.1"
                     /translation="MENQHNSKLLTLLLIGLAVFIQQSSVIAGVNVSIADFITLLILV
                     YLLFFANHLLKANHFLQFFIILYTYRMIITLCLLFFDDLIFITVKEVLASTVKYAFVV
                     IYFYLGMIIFKLGNSKKVIVTSYIISSVTIGLFCIIAGLNKSPLLMKLLYFDEIRSKG
                     LMNDPNYFAMTQIITLVLAYKYIHNYIFKVLACGILLWSLTTTGSKTAFIILIVLAIY
                     FFIKKLFSRNAVSVVSMSVIMLILLCFTFYNINYYLFQLSDLDALPSLDRMASIFEEG
                     FASLNDSGSERSVVWINAISVIKYTLGFGVGLVDYVHIGSQINGILLVAHNTYLQIFA
                     EWGILFGALFIIFMLYLLFELFRFNISGKNVTAIVVMLTMLIYFLTVSFNNSRYVAFI
                     LGIIVFIVQYEKMERDRNEE"
     gene            21488..22918
                     /locus_tag="O2701_001331"
     CDS             21488..22918
                     /locus_tag="O2701_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCZ4293234.1"
                     /translation="MKSDSLKENIIYQGLYQLIRTMTPLITIPIISRAFGPSGVGIVS
                     FSFNIVQYFLMIASVGVQLYFNRVIAKSVNDKRQLSQQFWDIFVSKLFLALTVFAMYM
                     VVITIFIDDYYLIFLLQGIYIIGAALDISWFYAGTEKFKIPSLSNIVASGIVLSVVVI
                     FVKDQSDLSLYVFTIAIVTVLNQLPLFIYLKRYISFVSVNWIHVWQLFRSSLAYLLPN
                     GQLNLYTSISCVVLGLVGTYQQVGIFSNAFNILTVAIIMINTFDLVMIPRITKMSIQQ
                     SHSLTKTLANNMNIQLILTIPMVFGLIAIMPSFYLWFFGEEFASTVPLMTILAILVLI
                     IPLNMLISRQYLLIVNKIRLYNASITIGAVINLVLCIILIYFYGIYGAAIARLITEFF
                     LLIWRFIDITKINVKLNIVSTIQCVIAAVMMFIVLGVVNHYLPPTMYATLLLIAIGIV
                     VYLLLMMTMKNQYVWQILRHLRHKTI"
BASE COUNT         7691 a         3565 c         4469 g         7317 t
ORIGIN      
        1 agaaatagtt atcgatattt gaaaaatcga agaatattga ttgatattat agaatatgag
       61 tcggtgttat ttgtatataa agtattaata tgtaattaag aaagtcgacg attgttaaca
      121 ataacaacaa ctatatgtat aaaaaaacct cgcaacggtt agttaactga atcatttagc
      181 taacttcgtt gtgaggtcat tttgttttaa taatatcgtt ataacttttt cacggttaat
      241 aataagtata tgaagaatgg ggcaccaaaa gcagcaataa atacacctgc tggcacttct
      301 ttaggcaaga ataaggtacg cccaattaag tctgcaataa caattgatat ggcaccaatc
      361 attgctgaca ttagtaactt tttagcataa cttccgcgaa cgattgtttt cgcgatatgt
      421 ggtgcgatta aaccgacaaa cccaatgtta cctactaaac tgattgccat agatacgagt
      481 atagtagaag tgattaattg gattagtttc atacgttgta catgtaagcc taagccaatc
      541 gctacagggt catcaagtat agatattttc atttttggta taacaagaaa taacaacggc
      601 acaacagcta aaataaccat acccaaaatg attgtatctt taaacgtagc accgtaaaga
      661 cttccgacta gccatgtata agctttggca gcagataatt gcttcgttgt aatgagtaat
      721 ccttggacaa gcgcaataaa caacgtttgc atcgaaatac cgatgattat gagtgttgtc
      781 gggcgtattt gtcctttcgt ttgaaacact aatagtatca tcattgcaac tgcgccacct
      841 aatactgcaa atagtggaag taaatgtatt gttaaatggc tgaaaaatgc aataaagaca
      901 acagcactta agctagcacc acctgtgata ccgataatat caggtgaggc aattggattt
      961 tttaatacat tttgcaacat taaaccactc attcctagtg cggcacctgc taaaatcgca
     1021 agtgtaatgc gaggtaagcg taatacttct aaagtgaatt gatccatact gtcatttgga
     1081 tttataaagt acatcagtac gcgttgtaat ggtataaagc ttgaaccaat catcatactt
     1141 accactgaaa cgatggctaa aaagattaac gcgaagatga gatggtaatt gtctttttta
     1201 ttaatctttt cggtcataag cgttgacgtc ctttcttcat aatatagatt aagacaatag
     1261 cgccaatgac agcggtaacg acaccgatag gcaactctag tggcttaatt attatacgag
     1321 caacaatgtc tgaaatgatc attaggattg ctccagctaa tgcagtaaaa ggaattaaat
     1381 acttatagtt tggtggtaat aatcgtttgc taatattcgg tacgataaga cccacaaaga
     1441 cgattgatcc agctacggct accgaaatac cggctaacat actgatgagc ataataatca
     1501 tccatttgat taattttatg ttttgaccga ggccggttgc aatgtcgtca cttgtcatca
     1561 agatgttgat gtgtgcagcc atgctaaatg caattaaaat aagtatcaat acaagcggaa
     1621 taatccatgg gatatcccaa atattacgta atgaaacgga gccacttaac caaaataata
     1681 ggccttgtaa gtctgtttcg ttcataataa gtatgccttg agtaaaggct gtaaatagca
     1741 tcgcaatcgc agcacctgcc aaaatgacac ggtgaggtga gaatagtgtt tgtctaaaca
     1801 tacctagtgc aacaactaat acagtaacaa caatagcccc caaaaatgca ataactacaa
     1861 tcattttaaa agattgaatt tggataaatg taatactaaa aatgacaaaa aatactgcgc
     1921 ctgcattgac accgaaaagc cctggtgagg ctattgggtt tcgtgtaagt gcttgcatca
     1981 acaaacctga gacagcaagg gcagcaccag tcaataacgc aatgattgtt ctcgacgccc
     2041 gtgcaccagt gacaacatca tgtaaatcgt tttcactatc aaagttgaat aacgcctgta
     2101 tcaccgtacc tggtgacaca agcgtatttc caatcattaa acttaagata gctactattg
     2161 caagacataa accagcaata acgatttggt attttggttt aagtagcatc gtaaaactcc
     2221 ttaattattt tgattgtttt tcaatattta acttttcata taaatcgtca ataagtttta
     2281 atgaagattt atatccgcca gctaagttcc aagtgatttc atctaaatca tcagatactt
     2341 ggttgttttt aactgcgtct aaatttttcc actctttact tgaagtccat tcgctttcag
     2401 tctttttaac taatgcagca tctttcgcat ttggatctga ttttactaca aaaatatgat
     2461 cagcgttcat taatggaatg ctttctttag atgtaagttg gataatatct ttaccattat
     2521 caacttgttt ttgtaagtct ttattacgtt tgaatcctaa atcatttaag atttcaccag
     2581 catatccacc agcataaatt cttgtatgat cagcacggaa gttaacaact gaagctttca
     2641 atggccatgc atctttatac tttgcttttg catctttttg gaatgcagct actttatcat
     2701 cgtacttttt aagtaaatct tcagcttctt tttctttccc taaagctttc cccattaact
     2761 tagttgtatc tttgaatttg aaaactgtat cagtagaaac tgttggtgcg attttagata
     2821 attgatcgta aactttttca tttctaactt ttgacgcgac aattaagtcc ggttttaatt
     2881 tagagatttc ctctaagtta ggtgcaggtt cttgacctac aatcttagta tcttttaaat
     2941 cattttttat gtattcgaat ttcggttttt gtgtccatga ttctacagca cctacaggtt
     3001 taacacctaa agatacagcg acgtcagtgg caccttgata tagcgtaaca acacgctttg
     3061 gtttcccttt aatttcagtt gtacccattg catgtttaat tgaagttgtt tccttatctt
     3121 tgttatcaga tgattgttta tttgaattcc cactacatcc tgctaaaaca agtaggaaag
     3181 caagcgtaac aacaagcatt ttaattactt tattcattga ctaattagcc tccttcgtga
     3241 tgtatgacaa tgagaatcat tatcacgatt tagtatgaat taaatttttt cctaagtcaa
     3301 taaaatattt atgatttaca tgcaacttat aattatttga catataaatg cataaaaaat
     3361 ataatcctaa ttacttgata gtgagaatca ttatcaatta ggtaacacac aatattatag
     3421 aattttaaat ttgaggagga agcgcttttg attgaaaaaa gtcaagcatg tcacgattca
     3481 ttgttagatt ctgtagggca aacacctatg gttcaacttc atcaactatt tccgaaacat
     3541 gaagtgtttg caaagttaga gtatatgaat cctggaggca gcatgaaaga tcgacctgcc
     3601 aagtacatca ttgaacatgg tattaaacat ggtttaatca ctgagaatac acatttaatt
     3661 gaaagtactt ctggtaattt aggcattgcg ttggcaatga tagctaaaat caagggatta
     3721 aaactcacgt gtgttgttga tcctaaaata tcaccaacaa atttgaaaat tattaaaagt
     3781 tatggtgcca atgtagaaat ggttgaagaa cctgatgcac atgggggtta tttaatgact
     3841 cgtattgcaa aggtgcaaga actgttagcc actattgacg atgcatattg gattaatcaa
     3901 tatgcgaatg agttaaattg gcaatcccat tatcatggtg caggcacaga gattgttgaa
     3961 acaattaagc aacctataga ttattttgtc gcgccagtca gcacgacagg tagcattatg
     4021 ggtatgagta gaaaaataaa agaagtgcat ccaaacgcac aaattgttgc tgttgatgcg
     4081 aaagggtcag tcatttttgg tgacaaacct attaatagag aattacctgg tatcggtgct
     4141 agtcgtgtac ccgaaatatt gaatagatca gaaattaatc aagtgatcca tgtagatgat
     4201 tatcaatctg ctttgggctg tcgaaaactg attgattatg aaggcatatt tgccggaggt
     4261 tcaacaggtt cgattattgc agcgattgag cagttgataa cgtcaattga agaaggtgca
     4321 acaattgtca cgattttacc agatcgaggc gatcgttact tagatttagt ttattcagat
     4381 acatggttag aaaaaatgaa atcaagacaa ggagttaaat cagaatgaat agagagatgt
     4441 tgtatttaaa tagatcagat attgaacaag cgggaggtaa tcattcacaa gtttatgtgg
     4501 acgcattaac agaagcatta acagcccatg cgcacaatga ttttgtacaa ccgcttaagc
     4561 cgtatttaag acaggatcct gaaaatggac acatcgcaga tcgaattatt gcaatgccaa
     4621 gtcatatcgg tggtgaacac gcaatttcag gtattaagtg gataggtagt aagcacgaca
     4681 atccatcgaa acgtaatatg gagcgtgcaa gtggcgtcat tattttgaat gatccagaaa
     4741 cgaattatcc aattgcagtt atggaagcaa gtttaattag tagtatgcgt actgcagcag
     4801 tttcagtgat tgcagcaaag catttggcta aaaaaggatt taaagactta acaatcattg
     4861 gatgcgggct aatcggagac aagcaattac aaagtatgtt agagcaattc gatcatattg
     4921 aacgcgtgtt tgtttacgat caattctctg aagcatgtgc acgctttgtt gatagatggc
     4981 aacaacagcg tccggaaatt aattttattg cgacagaaaa tgctaaagaa gcagtatcaa
     5041 atggtgaagt agtcattaca tgtaccgtaa cggatcaacc atacattgaa tatgattggt
     5101 tacaaaaggg tgcatttatt agcaacattt ctatcatgga tgtgcataaa gaagtcttta
     5161 ttaaagctga caaagtcgta gtagatgact ggtcacaatg taatcgagaa aagaaaacta
     5221 ttaaccaatt ggtgttagaa ggtaaattca gcaaagaagc tcttcatgct gaactaggac
     5281 aacttgtgac aggtgacata ccaggacgtg aagacgatga tgagatcata ttacttaatc
     5341 cgatgggtat ggctatcgaa gatatttcaa gtgcttattt tatttatcaa caggcacaac
     5401 aacaaaatat tgggacaaca ttgaacctat attaagaatg cgaggtgtct gaacattgca
     5461 gaatcataca gcagtcaata cagcacaagc gataatatta agagatttag ttgatgcatt
     5521 attatttgaa gatatagccg gaattgtatc gaatagtgag attactaaag aaaacggaca
     5581 aacgcttttg atatacgaac gtgaaacaca acaaataaag atacctgttt attttagtgc
     5641 tttaaatatg tttcgttacg aaagttcaca accaattacg atagagggaa gggtgtctaa
     5701 gcaaccttta acggcagctg aattttggca aacaattgct aatatgaatt gtgatctaag
     5761 tcatgaatgg gaagtggctc gcgttgaaga aggactgact actgctgcca cacagcttgc
     5821 taaacaatta tcagaattag atttagcgtc acatcctttt gtgatgtcag agcagtttgc
     5881 aagtttaaaa gatcgtccat ttcatccatt agctaaagaa aaaagaggat taagagaagc
     5941 ggattatcaa gtgtatcaag ctgaattaaa tcaatcattt cctttaatgg ttgcagcagt
     6001 taaaaagaca catatgattc atggcgatac tgcaaatatc gatgaattag aaaatttgac
     6061 agtacctata aaagaacaag cgacagacat gttaaatgat caagggttat caatagatga
     6121 ctatgtacta tttccggtac atccttggca atatcagcat attctgccga acgtctttgc
     6181 gaaagagatt agtgaaaagt tggttgtact attaccgtta aaatttggag attatctgtc
     6241 gtcttcaagt atgcgttcat taattgatat tggcgcaccg tataaccatg tcaaagtacc
     6301 atttgcaatg cagtcattag gcgcattaag gctaacgcct acgcgttaca tgaaaaacgg
     6361 agaacaagca gaacaattat tacgtcagct tatagaaaaa gatgaagcac tagctaagta
     6421 tgtcatggtt tgtgatgaaa cagcttggtg gtcatatatg ggtcaagata atgatatttt
     6481 caaagatcaa ttaggtcatc taactgttca gctaagaaag tatcccgaag tgctagccaa
     6541 aaatgatacg caacagctag tgtcaatggc agcactcgcg gcaaatgatc gcactttata
     6601 tcaaatgatt tgtggaaaag ataatatttc taaaaatgat gtcatgacgt tatttgaaga
     6661 tatcgcgcaa gtctttttaa aggtaacact atcatttatg caatacggcg cattaccaga
     6721 gttgcatggt caaaatatat tgttgtcatt tgaagatgga cgtgtacaaa aatgcgtgtt
     6781 acgtgatcat gatactgtca gaatttataa accatggcta acagcacatc agctttcatt
     6841 gccgaagtat gtcgtcagag aagatacacc taatacgcta attaatgagg atttggaaac
     6901 attctttgct tattttcaaa cattagctgt atcggtaaat ctatatgcca ttattgatgc
     6961 aattcaagat ttatttggtg taagtgagca tgaacttatg tcgttgttaa aacaaatttt
     7021 aaaaaatgaa gtggcaacta tttcctgggt tacaactgat cagctagctg tcagacacat
     7081 tttatttgat aaacagacgt ggccattcaa acaaatttta ttaccattgc tatatcaacg
     7141 tgatagtggt ggaggtagta tgccttcagg tttaactacc gtaccaaatc caatggtgac
     7201 atatgattaa tcagtctata tggcgcagta actttcgcat tttatggctc agtcagttta
     7261 tagcgattgc tggactgaca gtacttgtgc cattattgcc aatttatatg gcatcactac
     7321 aaaatctatc agtcgtagaa atacagttgt ggagtggtat agcgattgct gctccagctg
     7381 taacgacgat gatagcttcg ccgatatggg ggaagctagg tgataagatc agccgaaaat
     7441 ggatggtgtt aagagcgtta cttggtttgg cggtatgctt atttttaatg gcattgtgta
     7501 cgacaccatt acagtttgta cttgtgaggt tattgcaggg actatttggt ggtgttgttg
     7561 atgcatcaag tgcgtttgcg agtgcagagg cgccagctga agatcgtgga aaggtattag
     7621 gaagactgca aagttcagtc agcgcagggt ctcttgtggg gccattaatt ggcggtgtta
     7681 cagcttcgat attaggtttt agtgcgttac tgatgagtat tgccgttatt acttttattg
     7741 tctgtatttt cggtgcatta aaattgattg aaacgacaca tatgccaaaa tcacaaacac
     7801 caaatattaa taaaggtatt cgccgttcat ttcaatgtct attatgcaca caacaaacat
     7861 gtcgatttat tatcgttggc gttttagcaa actttgctat gtatggcatg ctaactgcat
     7921 tatcaccact tgcttcatca gtgaatcata cagcgataga tgaccgtagt gtgattggat
     7981 ttttacagtc cgcattttgg acggcttcga tattaagcgc gcctttatgg ggacgcttta
     8041 atgataaatc atatgttaaa tcagtatata tatttgccac gattgcatgt ggttgtagtg
     8101 cgatactgca aggtttagcg acgaatatag agtttttaat ggctgcaaga atacttcaag
     8161 gattaacata tagtgcattg attcaaagtg tcatgtttgt tgtcgtgaat gcgtgtcatc
     8221 aacaacttaa aggcacattt gttggaacga cgaacagtat gttagttgtt ggtcaaatta
     8281 ttggcagtct tagtggcgct gccattacaa gttatactac accagctact acgtttatcg
     8341 ttatgggcgt agtatttgca gtaagtagtt tatttttaat ttgttcaacc atcactaatc
     8401 aaatcaacga tcacacatta atgaaattat gggagttgaa acaaaaaagt gcaaaataaa
     8461 gaattaatac aacatgcagc gtatgcggct atcgaacgca ttttaaatga atattttaga
     8521 gaagaaaatt tatatcaagt accacctcaa aatcatcaat ggtctataca attatcagag
     8581 ctcgaaactt taacgggtga atttcgctat tggtctgcga tggggcatca tatgtatcat
     8641 ccagaggtat ggcttatcga tggaaaaagt aaaaaaataa caacttataa agaagcaatt
     8701 gcgcgtattt tgcaacatat ggctcaaagt gcagataatc aaacggcagt gcaacaacat
     8761 atggcgcaaa ttatgtctga catcgataat agcattcatc gcacggcacg ttatttgcaa
     8821 agtaacacaa tagactacgt agaggatcgt tatatcgttt cagaacaatc tttatactta
     8881 ggtcatccat ttcatccgac tcctaagagt gcaagtgggt tttcagaagc agatttagag
     8941 aaatatgcac ccgaatgtca tacatcattc caattgcatt atttagctgt gcatcaagat
     9001 gttctgctca cgcgctatgt agaaggtaaa gaagatcagg ttgagaaagt gttgtatcaa
     9061 ttagcagaca tagatatatc agagataccc aaagatttta ttttattacc aacacatcct
     9121 tatcaaatca atgtgttgcg acagcatcca cagtatatgc aatatagtga acaaggttta
     9181 ataaaagacc ttggcgtttc cggtgattca gtgtacccga cgtcttcggt tagaactgta
     9241 ttttcaaaag cattaaacat ttatttaaaa ttaccgatac acgttaaaat cactaatttt
     9301 atacgtacga atgaccttga acagattgaa cggacaattg atgccgcgca agttatcgca
     9361 tcagtcaaag atgaggttga aacaccccat tttaaattga tgtttgaaga aggatatcgt
     9421 gcattgttac cgaatccatt agggcaaaca gttgaacctg aaatggattt attaacaaat
     9481 agtgccatga ttgttcgtga agggataccg aattaccatg ctgataaaga tattcatgta
     9541 ttggcgtcat tatttgaaac gatgcctgat tcaccgatgt ctaagttatc acaagtgatt
     9601 gagcaaagtg gtttagcacc agaagcatgg cttgaatgtt atttgaatcg tacattattg
     9661 ccgatattaa agctgtttag taacacaggc attagtctag aagcacatgt acaaaataca
     9721 ttaattgaat taaaagatgg catacccgac gtatgctttg tcagagatct tgaaggcatt
     9781 tgtctatcta gaacgattgc tactgaaaaa cagcttgtgc caaatgttgt ggcagcatca
     9841 agccctgttg tatatgcaca tgatgaagca tggcatcgtc ttaaatatta cgttgtagta
     9901 aatcacttag gacatttagt atcaactatt ggtaaagcga ctagaaatga agttgtgtta
     9961 tggcaacttg tagcgcatcg tcttatgact tggaaaaaag aatacgcgaa taacgcagta
    10021 tttgttgact gtgtagaaga tttatatcaa acgccgacca ttgcggctaa agcgaatttg
    10081 atgagtaaat tgaatgattg tggtgcaaac cctatttata cacatatacc aaatccaatt
    10141 tgtcataaca aggaggtatc gtattgtgaa tcaaacaatt cttaatcgtg taaagactag
    10201 agtgatgcac caactggtat catcacttat ttatgagaat attgttgtgt ataaagcgtc
    10261 atatcaagac ggtgtcggtc attttacaat agaaggacat gattcagagt atcgttttac
    10321 tgctgaaaag acacatagct ttgatcgtat acgtatcaca tcaccaattg agcgtgtcgt
    10381 aggagatgag gcagatacaa caacagacta tacacaatta ttgagagagg ttgtatttac
    10441 atttcctaaa aatgatgaaa agctagaaca atttattgtc gagttattac agacagaatt
    10501 aaaagatacg caaagtatgc agtatcgaga atcaaaccca ccagcaacac ctgagacatt
    10561 taacgactat gaattttatg cgatggaagg gcatcagtat catccaagtt acaaatcacg
    10621 tttaggattt acgttgagtg ataatttgaa atttggtcct gattttgtac caaacgttaa
    10681 actgcagtgg ttagctatcg acaaagataa agtagaaacg acggtatcaa gaaatgttgt
    10741 agttaacgaa atgttacgtc aacaagttgg cgataagact tatgaacatt ttgtacagca
    10801 gattgaagca tctggcaaac atgtaaatga tgttgagatg atacctgtac acccatggca
    10861 gtttgaacat gtcatccaag ttgatttggc tgaagaaagg cttaatggca cagtactatg
    10921 gttaggggaa agtgatgagc tatatcaccc tcaacaatcg attcgtacga tgtcgccaat
    10981 agacacgaca aaatattatt taaaggtacc aataagtata acgaacactt caacgaaacg
    11041 agtgttggcg cctcatacaa ttgaaaatgc agcgcaaatt acggattggt taaagcagat
    11101 acagcaacaa gatatgtatt taaaagatga attaaagaca gtttttctag gggaagtctt
    11161 aggacagtct tatttaaata cacaactttc gccttataaa caaactcaag tttatggtgc
    11221 gttaggtgtt atatggcgtg aaaatatata tcatatgtta atcgatgaag aggatgcgat
    11281 accatttaat gcactttatg caagtgataa ggatggtgta ccattcattg aaaattggat
    11341 taaacaatat ggttctgaag cttggacaaa gcaattttta gctgtagcga ttcgtccaat
    11401 gattcatatg ctttattatc acggtattgc ctttgaatcg catgcacaaa atatgatgct
    11461 cattcatgaa aatggttggc ctacacgtat tgccttaaaa gatttccatg atggtgttcg
    11521 ttttaagcgt gagcatttaa gtgaagctgc ttcacacctg acattaaagc caatgccaga
    11581 agcacataaa aaagtgaata gtaattcatt tattgaaaca gatgacgaac gtttagtacg
    11641 cgacttttta catgatgcat ttttctttat taatatcgcc gaaatcatct tatttattga
    11701 aaagcaatat ggtatcgatg aggagctgca atggcaatgg gttaaaggca tcatcgaggc
    11761 gtatcaagaa gcatttccag agttgaataa ctatcaacat ttcgatttgt ttgaacctac
    11821 gattcaagtt gaaaagttaa cgacacgtcg attattaagt gactccgagt taagaattca
    11881 tcatgttaca aatccattag gtgtaggagg tatcaatgat gcaacaacta tctctgaaac
    11941 atagattaaa caatggtgat tcagtttatg gcatttttaa ttctataccg gacccattga
    12001 tgatcgaggt tatcgcagca agcgggtatg actttgttgt gattgataca gaacacgtgg
    12061 cgattaatga tgagacacta gcgcatttaa ttcgtgcagc tgaagcagcg catattatac
    12121 caattgtacg tgtcactgca gtgatagata gagatatcat taaagtgttg gatatgggtg
    12181 cgagaggtat tattgtgcca cacgttaaag atcgtgagac agttgagcat attgtgaaat
    12241 taagtcgtta ttacccgcaa ggattaagaa gtttgaatgg tggtcgcatg gcaagatttg
    12301 gacgtacacc attacttgat gcaatggaga tggctaatga gcatattatg gtgattgcca
    12361 tgatagaaga tgttgaaggg gttatggcca ttgacgatat agcacaagtc gaaggtttag
    12421 acatgatagt cgaaggtgcc gcagatttat cgcagtcact tggcatacca tggcaaacgc
    12481 gtgatgatca agtaacatca catgttcaac atatttttga agttgtgaat gcacatggta
    12541 aacatttttg tgcattacca cgtgaagatg aagatattgc aaaatggcag gcacaaggtg
    12601 tacaaacatt tattttaggt gatgatcgcg gaaaaatata tcgccattta agtgcatctc
    12661 tagcgacgtc taaacagaaa ggggatgaag gctaatgcgt atagttcaac ctgttattga
    12721 acaattaaaa gcacaatctc atccagtttg tcattatatc tatgatttag tcggactgga
    12781 acatcatttg caacatatta catcgtcatt gccgagtaat tgtcaaatgt actatgcaat
    12841 gaaagcaaat agtgaacgaa aaatcctaga tacaattagt cagtatgttg aaggattcga
    12901 agttgcatct caaggtgaaa tagcaaaagg tcttgctttt aaaccagcaa atcatattat
    12961 ttttggtggc cctggtaaga cagacgagga actaagatat gcagtaagtg aaggtgttca
    13021 gcgtattcat gttgaaagta tgcatgaatt acaacggcta aatgccatct tagaagatga
    13081 agataagaca caacacattt tattgcgtgt taatttagca ggaccatttc ccaatgcaac
    13141 gttgcatatg gcaggacgcc caacacaatt tggtatttct gaagacgaag ttgatgatgt
    13201 cattgaagct gcgctcgcaa tgccaaagat tcatctagat ggatttcatt ttcattctat
    13261 ttctaacaat ttagactcga atttacatgt cgatgtagtg aaactttatt ttaaaaaagc
    13321 aaaggcatgg tctgaaaaac atcgatttcc actcaaacat atcaatcttg gtggtggcat
    13381 aggcgttaac tatgcagatt taactaacca atttgaatgg gataattttg tagaacgttt
    13441 taaaacactt atcgttgagc aagaaatgga agatgtgaca ttgaactttg aatgtgggcg
    13501 ctttattgtg gcacatattg gttactatgt gacagaagtg ctagacatta agaaagtaca
    13561 tggtgcttgg tatgccatat taagaggagg tacgcaacaa tttagactgc cggtatcttg
    13621 gcaacataac catccttttg acatttatcg ctataaggac aatccatatt catttgaaaa
    13681 agtttcaatt tcgagacagg acacaacgtt agtcggtcaa ttatgtacac cgaaagatgt
    13741 ctttgctaga gaagtacaga tagacgcaat cagtacaggc gacgttattg ttttcaaata
    13801 tgcaggtgca tacggatggt ctatttcaca tcacgatttc ttaagccatc cacatcctga
    13861 atttatttat ttaacgcaaa caaaggagga tgaataacta ttgaatcata ttcatgaaca
    13921 tttaaaattg gtaccagtag ataagattga tcttcacgaa acattcgaac ctttaagatt
    13981 ggaaaaaacg aaaagtagta ttgaagcaga tgattttata cgtcatccta ttttagtgac
    14041 agcgatgcaa catggtagat atatggttat agatggtgtg catcggtata caagtttgaa
    14101 agcgttagga tgtaagaaag ttccagtgca agaaatccat gaaacacaat attcaattag
    14161 tacatggcaa cataaagttc catttggtgt gtggtgggaa acgttacaac aagaacatcg
    14221 cttgccatgg actactgaga caagacaaga agcgccattt attacaatgt gtcatggtga
    14281 tacagaacaa tatttgtata caaaagattt aggcgaagca cattttcaag tatgggaaaa
    14341 ggttgtcgca agttatagtg gttgttgttc tgtagagaga attgcacaag gtacatatcc
    14401 ttgtctttct caacaagatg tactcatgaa gtatcagcca ttgagttata aggaaattga
    14461 agcggttgtt cataaagggg aaactgtgcc agcaggtgtg acacgcttta atatttcagg
    14521 acgatgtctt aatcttcaag taccactggc attacttaaa caagatgatg atgttgaaca
    14581 actgcgcaat tggaagcagt ttttagcaga taagtttgcc aatatgagat gctatactga
    14641 aaaagtatac ttggtggagc aatagtttta ctgtgatgtt gagggaaata tgatgattta
    14701 gcgtattgat agcgaaaata taataaaaca atatagtgtg gagaactttt gatattttat
    14761 aaatattgaa gttctccatt tttgtatttt gcatataaaa attaaataaa ataaggtata
    14821 ttaaggtaaa gtataaattt taaataaatg gggaatgagt atgagctcaa ttataggaaa
    14881 aatagcaatt tggataggca tcgtagctca aatatatttt agtgtcgttt ttgttaggat
    14941 gatatctatt aatattgctg gaggatctga ttacgaaaca atttttttat taggattaat
    15001 attggctctt ttcactgttt taccaaccat ctttactgcg atttatatgg aaagttactc
    15061 tgtaatcgga ggtgcacttt ttattgttta tgctattatt gcactgtgtt tatataattt
    15121 cctttcgtca attttatggc tgattggtgg tattttgctg atttggaata aatactcaaa
    15181 agatgaatcg acagacgaaa atgaaaaagt tgatattgaa agtacagaga atcaatttga
    15241 atctaaagat aaaatcacta aagaataaag agaatattta aggtaaagta taaattttaa
    15301 ataaatgggg aatagacatg gaaaaaaatg tagaaaaatc attcataaag ataggtttat
    15361 attttcaaat agcttatata gtactcatgg ctataacttt atgtgggttt gtaatttgct
    15421 atggactaat tttcggcctt ttctatttat tatcaggtag cagagctgat tatttaatag
    15481 taacaatagt tatatcggca ataatttcta tatttgtaat tatactttca atcgtacctg
    15541 tcatcgtatt ggcatctgac ttatttaaag aaaggatttc aaaaggtgtc atattaattg
    15601 tattggctat tatcgcttta gtattatgca actttgtatc tgcaatactc tggtttgttt
    15661 cagccatatc tattttaggt agaaaaaaat tagtagctgc agcagatact accactattc
    15721 aaaaaagtaa agggaacgca aatcaagcat cacataaaga cacgtgtaaa aaggaacttg
    15781 atagtcaaga catgatggaa catcctgagg ttaaaaatcc cacgactaaa aaccttgaag
    15841 gatttaacga agaaatacat aaagatgaag ctacaactaa agttgtcagt gataacacgg
    15901 aaccgcctat tgaatcaaaa gaccatgtct cgaaaaaaga ttgatgacaa actaatcgag
    15961 agacttaaaa aaataatatt caacataaga acttttaaaa cgacatttaa acgcattgcc
    16021 aatcactaat ggtagtgcgt ttaactatac cttaaatatc tgaatatttt gttaaatgga
    16081 gctacctttg ttgtactatt caaatgaaga ggagtaaaat gtaattaaag gaaagaaatt
    16141 tgaggagtga tctttatgac aaacaacaaa gtagcattag taactggcgg agcacaaggg
    16201 attggtttta aaattgcaga acgtttagtg gaagatggtt tcaaagtagc agttgttgat
    16261 ttcaatgaag aaggggcaaa agcagctgca cttaaattat caagtgatgg tacaaaagct
    16321 attgctatca aagcagatgt atcaaaccgt gatgatgtat ttaacgcagt aagacaaact
    16381 gccgcgcaat ttggcgattt ccatgtcatg gttaacaatg ccggccttgg accaacaaca
    16441 ccaatcgata caattactga agaacagttt aaaacagtat atggcgtgaa cgttgcaggt
    16501 gtgctatggg gtattcaagc cgcacatgaa caatttaaaa aattcaatca tggcggtaaa
    16561 attatcaatg caacatctca agcaggcgtt gagggtaacc caggcttgtc tttatattgc
    16621 agtacaaaat tcgcagtgcg aggtttaaca caagtagccg cacaagattt agcgtctgaa
    16681 ggtattactg tgaatgcatt cgcacctggt atcgttcaaa caccaatgat ggaaagtatc
    16741 gcagtggcaa cagccgaaga agcaggtaaa cctgaagcat ggggttggga acaatttaca
    16801 agtcagattg ctttgggcag agtttctcaa ccagaagatg tttcaaatgt agtgagcttc
    16861 ttagctggta aagactctga ttacattact ggacaaacaa ttattgtaga tggtggtatg
    16921 agattccgtt aataatcatc cactaatgat aaataaatcc ttattgttaa gtttaatcac
    16981 ttagcagtaa ggatttttta gtgcacttag aagggagtgt attggtagaa aattaataag
    17041 cgaagttctt aagtgagtta tgatgtcaca gtctaatgca tcagttgaaa gcattattag
    17101 tattaacaca cccaagatat tataaaacat cacaaaaaca ccactatcta atttatctca
    17161 ataaaaattc acaaagttat ctcattttat ttttataaat aaaaaatatc gataaaaagc
    17221 ttacaatact ttatgttttt atgatatatt tttaatgtat aaatgaggtg gaagatttgg
    17281 aaagagtttt gataactggt ggggctggtt ttattgggtc gcatttagta gatgatttac
    17341 aacaagatta tgatgtttat gttctagata actatagaac aggtaaacga gaaaatatta
    17401 aaagtttggc tgacgatcat gtgtttgaat tagatattcg tgaatatgat gcagttgaac
    17461 aaatcatgaa gacatatcaa tttgattatg ttattcattt agcagcatta gttagtgttg
    17521 ctgagtcggt tgagaaacct atcttatctc aagaaataaa cgtcgtagca acattaagat
    17581 tgttagaaat cattaaaaaa tataataatc atataaaacg ttttatcttt gcttcgtcag
    17641 cagctgttta tggtgatctt cctgatttgc ctaaaagtga tcaatcatta atcttaccat
    17701 tatcaccata tgcaatagat aaatattacg gcgaacggac gacattaaat tattgttcgt
    17761 tatataacat accaacagcg gttgttaaat tttttaatgt atttgggcca agacaggatc
    17821 ctaagtcaca atattcaggt gtgatttcaa agatgttcga ttcatttgag cataacaagc
    17881 catttacatt ttttggtgac ggactgcaaa ctagagattt tgtatatgta tatgatgttg
    17941 ttcaatctgt acgcttaatt atggaacaca aagatgcaat tggacacggt tataacattg
    18001 gtacaggcac ttttactaat ttattagagg tttatcgtat tattggtgaa ttatatggaa
    18061 aatcagtcga gcatgaattt aaagaagcac gaaaaggaga tattaagcat tcttatgcag
    18121 atatttctaa cttaaaggca ttaggatttg ttcctaaata tacagtagaa acaggtttaa
    18181 aggattactt taattttgag gtagataata ttgaagaagt tacagctaaa gaagtggaaa
    18241 tgtcgtgaaa atgacattga agctgtccat aataataagg gttatgccta tcaaagaaaa
    18301 ttagacaaac tagaagaagt gagaaaaagc tattacccaa ttaaacgtgc gattgactta
    18361 attttaagca ttgttttatt atttttaact ttaccgatta tggttatatt cgccattgct
    18421 atcgtcatag attcgccagg aaaccctatt tatagtcagg ttagagttgg gaagatgggt
    18481 aaattaatta aaatatacaa attacgttcg atgtgcaaaa acgcagagaa aaacggtgcg
    18541 caatgggctg ataaagatga tgatcgtata acaaatgtcg ggaagtttat tcgtaaaaca
    18601 cgcattgatg aattaccaca actaattaat gttgttaaag gggaaatgag ttttattgga
    18661 ccacgcccgg aacgtccgga atttgtagaa ttatttagtt cagaagtgat aggtttcgag
    18721 caaagatgtc ttgttacacc agggttaaca ggacttgcgc aaattcaagg tggatatgac
    18781 ttaacaccgc aacaaaaact gaaatatgac atgaaatata tacataaagg tagtttaatg
    18841 atggaactat atatatcaat tagaacattg atggttgtta ttacagggga aggctcaagg
    18901 tagtcttaat ttacttaata agttcaaata aaagttatat tttaaagatt gtgaccaatt
    18961 gttacagtat aacgaggaat cccttgagac agtatcaaat ggcattaaga aatatgtgcc
    19021 atcattgatt tgcatggcta taaatactat tcatctgatg agatagccat gttaagaaat
    19081 tgaaagtata gcattaaagg ggtttgtaac agttgaaaat tatatattgt attactaaag
    19141 cagacaatgg tggtgcacaa acacatctca ttcaactcgc caaccatttt tgcgtacaca
    19201 atgatgttta tgtcattgta ggcaatcatg gaccaatgat tgaacaacta gatgcaagag
    19261 ttaatgtaat tattatcgaa catttagtag gtccaattga ctttaaacaa gatattttag
    19321 ctgtcaaagt gttagcacag ttattctcga aaattaaacc tgatgttatc catttacatt
    19381 cttccaaagc tggaacggtc ggacgaattg cgaagttcat ttcgaaatcg aaagacacac
    19441 gtatagtttt tactgcacat ggatgggctt ttacagaggg tgttaaacca gctaaaaaat
    19501 ttctatattt agttatcgaa aaattaatgt cacttattac agatagcatt atttgtgttt
    19561 cagatttcga taaacagtta gcgttaaaat atcgatttaa tcgattgaaa ttaaccacaa
    19621 tacataatgg tattgcagat gttcccgctg ttaagcaaac gctaaaaagc caatcacata
    19681 acaatattgg cgaagtagtt ggaatgttgc ctaataaaca agatttacag attaatgccc
    19741 cgacaaagca tcaatttgtt atgattgcaa gatttgctta tccaaaattg ccacaaaatc
    19801 taatcgcggc aatagagata ttgaaattac ataacagtaa tcatgcgcat tttacattta
    19861 taggcgatgg acctacatta aatgattgtc agcaacaagt tgtacaagct gggttagaaa
    19921 atgaagtcac atttttgggc aatgtcatta atgcgagtca tttattatca caatacgata
    19981 cgtttatttt aataagtaag catgaaggtt tgccaattag cattatagaa gctatggcta
    20041 caggtttgcc tgttatagcc agtcatgttg gcggtatttc agaattagta gctgataatg
    20101 gtatatgtat gatgaacaac caacccgaaa ctattgctaa agtcctggaa aaatatttaa
    20161 tagacagtga ttacatcaaa atgagtaatc aatctagaaa acgttattta gaatgtttta
    20221 ctgaggagaa aatgattaaa gaagtggaag acgtttataa tggaaaatca acacaatagt
    20281 aaattactaa cattgttact tatcggttta gcggttttta ttcagcaatc ttcggttatt
    20341 gccggtgtga atgtttctat agctgacttt atcacattac taatattagt ttatttactg
    20401 tttttcgcta accatttatt aaaggcaaat cattttttac agtttttcat tattttgtat
    20461 acatatcgta tgattattac gctttgtttg ctattttttg atgatttgat atttattacg
    20521 gttaaggaag ttcttgcatc tacagttaaa tatgcatttg tagtcattta tttctattta
    20581 gggatgatca tctttaagtt aggtaatagc aaaaaagtga tcgttacctc ttatattata
    20641 agcagtgtga ctataggtct attttgtatt atagctggtt tgaacaagtc ccctttacta
    20701 atgaaattgt tatattttga tgaaatacgt tcaaaaggat taatgaatga ccctaactat
    20761 ttcgcgatga cacagattat tacattggta cttgcttaca agtatattca taattacata
    20821 ttcaaggtcc ttgcatgtgg tattttgcta tggtctttaa ctacaacggg gtctaagact
    20881 gcgtttatca tattaatcgt cttagccatt tatttcttta ttaaaaagtt atttagtaga
    20941 aatgcggtaa gtgttgtgag tatgtcagtg attatgctga tattactttg ttttaccttt
    21001 tataatatca actactattt attccaatta agcgaccttg atgccttacc gtcattagat
    21061 cgaatggcgt ctatttttga agagggcttt gcatcattaa atgatagtgg gtctgagcga
    21121 agtgttgtat ggataaatgc catttcagta attaaatata cactaggttt tggtgtcgga
    21181 ttagtggatt atgtacatat tggctcgcaa attaatggta ttttacttgt tgcccataat
    21241 acatatttgc agatctttgc ggaatggggc attttattcg gtgcattatt tatcatattt
    21301 atgctttatt tactgtttga attatttaga tttaacattt ctgggaaaaa tgtaacagca
    21361 attgttgtaa tgttgacgat gctgatttac tttttaacag tatcatttaa taactcaaga
    21421 tatgtcgctt ttattttagg aattatcgtc tttattgttc aatatgaaaa gatggaaagg
    21481 gatcgtaatg aagagtgatt cactaaaaga aaatattatt tatcaagggc tataccaatt
    21541 gattagaacg atgacaccac tgattacaat acccattatt tcacgtgcat ttggtcccag
    21601 tggtgtgggt attgtttcat tttctttcaa tatcgtgcaa tactttttga tgattgcaag
    21661 tgttggcgtt cagttatatt ttaatagagt tatcgcgaag tccgttaacg acaaacggca
    21721 attgtcacag cagttttggg atatctttgt cagtaaatta tttttagcgt taacagtttt
    21781 tgcgatgtat atggtcgtaa ttactatatt tattgatgat tactatctta ttttcctact
    21841 acaaggaatc tatattatag gtgcagcact cgatatttca tggttttatg ctggaactga
    21901 aaagtttaaa attcctagcc tcagtaatat tgttgcgtct ggtattgtat taagtgtagt
    21961 tgttattttt gtcaaagatc aatcagattt atcattgtat gtatttacta ttgctattgt
    22021 gacggtatta aaccaattac ctttgtttat ctatttaaaa cgatacatta gctttgtttc
    22081 ggttaattgg atacacgtct ggcaattgtt tcgttcgtca ttagcatact tattaccaaa
    22141 tggacagctc aacttatata ctagtatttc ttgcgttgtt cttggtttag taggtacata
    22201 ccaacaagtt ggtatctttt ctaacgcatt taatatttta acggtcgcaa tcataatgat
    22261 taatacattt gatcttgtaa tgattccgcg tattaccaaa atgtctatcc agcaatcaca
    22321 tagtttaact aaaacgttag ctaataatat gaatattcaa ttgatattaa caatacctat
    22381 ggtctttggt ttaattgcaa ttatgccatc attttattta tggttctttg gtgaggaatt
    22441 cgcatcaact gtcccattga tgaccatttt agcgatactt gtattaatca ttcctttaaa
    22501 tatgttgata agcaggcaat atttattaat agtgaataaa ataagattat ataatgcgtc
    22561 aattactatt ggtgcagtga taaacctagt attatgtatt attttgatat atttttatgg
    22621 aatttacggt gctgctattg cgcgtttaat tacagagttt ttcttgctca tttggcgatt
    22681 tattgatatt actaaaatca atgtgaagtt gaatattgta agtacgattc aatgtgtcat
    22741 tgctgctgtt atgatgttta ttgtgcttgg tgtggtcaat cattatttgc cccctacaat
    22801 gtacgctacg ctgctattaa ttgcgattgg tatagtagtt tatcttttat taatgatgac
    22861 tatgaaaaat caatacgtat ggcaaatatt gaggcatctt cgacataaaa caatttaagt
    22921 accggtaatg ctatacttta gaaaattaag attaagaaga aaaggcaatt tcttattgaa
    22981 aaatggaagt tgtctttttt aattctcttt aaaagcggga aacaaaagca gttaaatgcc
    23041 tt
//
LOCUS       DALAGD010000043        22792 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.043, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000043 DALAGD010000000
VERSION     DALAGD010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22792
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.043"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            472..1383
                     /gene="rapZ"
                     /locus_tag="O2701_001332"
     CDS             472..1383
                     /gene="rapZ"
                     /locus_tag="O2701_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCZ4293235.1"
                     /translation="MDNNEKEKSKSELLVVTGLSGAGKSLVIQCLEDMGYFCVDNLPP
                     VLLPKFVELMEQGNPSLRKVAIAIDLRGKELFNSLVAVVDKVKSESDVIIDVMFLEAS
                     TEKLISRYKETRRAHPLMEQGKRSLINAINDEREHLSQIRSIANFVIDTTKLSPKELK
                     ERIRRYYEDEEFETFTINVTSFGFKHGIQMDADLVFDVRFLPNPYYVVDLRPLTGLDK
                     DVYNYVMKWKETEIFFEKLTDLLDFMIPGYKKEGKSQLVIAIGCTGGQHRSVALAERL
                     GNYLNEVFEYNVYVHHRDAHIESGEKK"
     gene            1380..2375
                     /locus_tag="O2701_001333"
     CDS             1380..2375
                     /locus_tag="O2701_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HCZ4293236.1"
                     /translation="MRQIKVVLIGGGTGLSVMARGLREFPIDITAIVTVADNGGSTGK
                     IRDEMDIPAPGDIRNVIAALSDSESVLSQLFQYRFEENQISGHSLGNLLIAGMTNITN
                     DFGHAIKALSKILNIKGRVIPSTNTSVQLNAVMEDGEIVFGETNIPKKHKKIDRVFLE
                     PNDVQPMEEAIDALREADLIVLGPGSLYTSVISNLCVNGISDALIHSDAPKLYVSNVM
                     TQPGETDGYSVKDHIDAIHRQAGQPFIDYVICSTQTFNAQVLKKYEEKHSKPVEVNKA
                     ELEKESINVKTSSNLVEISENHLVRHNTKVLSTMIYDIALELISTIPFVPSDKRK"
     gene            2485..3429
                     /gene="whiA"
                     /locus_tag="O2701_001334"
     CDS             2485..3429
                     /gene="whiA"
                     /locus_tag="O2701_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HCZ4293237.1"
                     /translation="MSFASEMKNELTRIDVEEMNAKAELSALIRMNGALSLSNQQFVI
                     NVQTENATTARRIYSLIKRVFNVEVEILVRKKMKLKKNNIYICRTKMKAKEILDELGI
                     LKDGIFTHEIDHSMIQDDEMRRSYLRGAFLAGGSVNNPETSSYHLEIFSQNESHAEGL
                     TKLMNSYELNAKHLERKKGSITYLKEAEKISDFLSLIGGYQALLKFEDVRIVRDMRNS
                     VNRLVNCETANLNKTVSAAMKQVESIKLIDKEIGIENLPDRLREIARIRVEHQEISLK
                     ELGEMVSTGPISKSGVNHRLRKLNDLADKIRNGEQIEL"
     gene            4003..4590
                     /gene="clpP"
                     /locus_tag="O2701_001335"
     CDS             4003..4590
                     /gene="clpP"
                     /locus_tag="O2701_001335"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCZ4293238.1"
                     /translation="MNLIPTVIETTNRGERAYDIYSRLLKDRIIMLGSQIDDNVANSI
                     VSQLLFLQAQDSEKDIYLYINSPGGSVTAGFAIYDTIQHIKPDVQTICIGMAASMGSF
                     LLAAGAKGKRFALPNAEVMIHQPLGGAQGQATEIEIAANHILKTREKLNRILSERTGQ
                     SIEKIQKDTDRDNFLTAEEAKEYGLIDEVMVPETK"
     gene            complement(4833..5735)
                     /locus_tag="O2701_001336"
     CDS             complement(4833..5735)
                     /locus_tag="O2701_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCZ4293239.1"
                     /translation="MKQYLITGGTGMVGSQLVNEIKKSDSHITILTRHDQISNDKKIS
                     YVNWAKSGWEHKVPQNIDVVINLAGATLNKRWTPEYKQTLMLSRIQSTQALYELFKSR
                     NKAPKALFNASATGYYPPDLFMSYTEVYKTLPFDFLSDIVYQWERFAQQFEQLGTRVV
                     IGRFGIILSNEGGALQTMKLPYEYYIGGKLGSGQQWYSWIHINDLIQAILFLINNESA
                     SGPFNLTAPIPERQNLFGYTLARAMHKPHETWAPSLAMRLILGQMSTVVLDTQKVLPN
                     KIQALGFQFKYSNLKMALEDLIKE"
     gene            6437..7066
                     /locus_tag="O2701_001337"
     CDS             6437..7066
                     /locus_tag="O2701_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4887 domain-containing protein"
                     /protein_id="HCZ4293240.1"
                     /translation="MSNQNYDYNKNEDGSKKKMSTTAKVVSIATVLLLLGGLVFAIFA
                     YVDHSNKAKERMLNEQKQEQKEKRQKENAEKERKKKQQEEKEQNELDSQANQYQQLPQ
                     QNQYQYVPPQQQAPTKQRPAKEENDDKASKDESKDKDDKASQDKSDDNQKKTDDNKQP
                     AQPKPQPQQPTPKPNNNQQNNQSNQQAKPQAPQQNSQSTTNKQNNANDK"
     gene            7826..8839
                     /locus_tag="O2701_001338"
     CDS             7826..8839
                     /locus_tag="O2701_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCZ4293241.1"
                     /translation="MKDLLQAQQKLIPDLIDKMYKRFSILTTISKNQPVGRRSLSEHM
                     DMTERVLRSETDMLKKQDLIKVKPTGMEITAEGEQLISQLKGYFDIYADDNRLSEGIK
                     NKFQIKEVHVVPGDADNSQSVKTELGRQAGQLLEGILQEDAIVAVTGGSTMACVSEAI
                     HLLPYNVFFVPARGGLGENVVFQANTIAASMAQQAGGYYTTMYVPDNVSETTYNTLLL
                     EPSVINTLDKIKQANVILHGIGDALKMAHRRQSPEKVIEQLQHHQAVGEAFGYYFDTQ
                     GQIVHKVKTIGLQLEDLESKDFIFAVAGGKSKGEAIKAYLTIAPKNTVLITDEAAAKI
                     ILE"
     gene            8892..9902
                     /gene="gap"
                     /locus_tag="O2701_001339"
     CDS             8892..9902
                     /gene="gap"
                     /locus_tag="O2701_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCZ4293242.1"
                     /translation="MAVKVAINGFGRIGRLAFRRIQEVEGLEVVAVNDLTDDDMLAHL
                     LKYDTMQGRFTGEVEVVDGGFRVNGKEVKSFSEPDASKLPWKDLNIDVVLECTGFYTD
                     KDKAQAHIEAGAKKVLISAPATGDLKTIVFNTNHQELDGSETVVSGASCTTNSLAPVA
                     KVLNDDFGLVEGLMTTIHAYTGDQNTQDAPHRKGDKRRARAAAENIIPNSTGAAKAIG
                     KVIPEIDGKLDGGAQRVPVATGSLTELTVVLEKQDVTVEQVNEAMKNASNESFGYTED
                     EIVSSDVVGMTYGSLFDATQTRVMSVGDRQLVKVAAWYDNEMSYTAQLVRTLAYLAEL
                     SK"
     gene            10041..11231
                     /locus_tag="O2701_001340"
     CDS             10041..11231
                     /locus_tag="O2701_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCZ4293243.1"
                     /translation="MAKKIVSDLDLKGKTVLVRADFNVPLKDGEITNDNRIVQALPTI
                     QYIIEQGGKIVLFSHLGKVKEESDKAKLTLRPVAEDLSKKLDKEVVFVPETRGEKLEA
                     AIKDLKEGDVLLVENTRYEDLDGKKESKNDPELGKYWASLGDVFVNDAFGTAHREHAS
                     NVGISTHLETAAGFLMDKEIKFIGGVVNDPHKPVVAILGGAKVSDKINVIKNLVNIAD
                     KIIIGGGMAYTFLKAQGKEIGISLLEEDKIDFAKDLLEKHGDKIVLPVDTKVAKEFSN
                     DAKITVVPSDSIPADQEGMDIGPNTVKLFADELEGAHTVVWNGPMGVFEFSNFAQGTI
                     GVCKAIANLKDAITIIGGGDSAAAAISLGFENDFTHISTGGGASLEYLEGKELPGIKA
                     INNK"
     gene            11353..12114
                     /locus_tag="O2701_001341"
     CDS             11353..12114
                     /locus_tag="O2701_001341"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCZ4293244.1"
                     /translation="MRTPIIAGNWKMNKTVQEAKDFVNALPTLPDSKEVESVICAPAI
                     QLDALTTAVKEGKAQGLEIGAQNTYFEDNGAFTGETSPVALADLGVKYVVIGHSERRE
                     LFHETDEEINKKAHAIFKHGMTPIICVGETDEERESGKANDVVGEQVKKAVAGLSEDQ
                     LKSVVIAYEPIWAIGTGKSSTSEDANEMCAFVRQTIADLSSKEVSEATRIQYGGSVKP
                     NNIKEYMAQTDIDGALVGGASLKVEDFVQLLEGAK"
     gene            12117..13634
                     /locus_tag="O2701_001342"
     CDS             12117..13634
                     /locus_tag="O2701_001342"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCZ4293245.1"
                     /translation="MAKKPTALIILDGFANRESEHGNAVKLANKPNFDRYYNKYPTTQ
                     IEASGLDVGLPEGQMGNSEVGHMNIGAGRIVYQSLTRINKSIEDGDFFENDVLNNAIA
                     HVNSHDSALHIFGLLSDGGVHSHYKHLFALLELAKKQGVEKVYVHAFLDGRDVDQKSA
                     LKYIEETEAKFNELGIGQFASVSGRYYAMDRDKRWEREEKAYNAIRNFDAPTYATAKE
                     GVEASYNEGLTDEFVVPFIVENQNDGVNDGDAVIFYNFRPDRAAQLSEIFANRAFEGF
                     KVEQVKDLFYATFTKYNDNIDAAIVFEKVDLNNTIGEIAQNNNLTQLRIAETEKYPHV
                     TYFMSGGRNEEFKGERRRLIDSPKVATYDLKPEMSAYEVKDALLEELNKGDLDLIILN
                     FANPDMVGHSGMLEPTIKAIEAVDECLGEVVDKILDMDGYAIITADHGNSDQVLTDDD
                     QPMTTHTTNPVPVIVTKEGVTLRETGRLGDLAPTLLDLLNVEQPEDMTGESLIKH"
     gene            13764..15068
                     /gene="eno"
                     /locus_tag="O2701_001343"
     CDS             13764..15068
                     /gene="eno"
                     /locus_tag="O2701_001343"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCZ4293246.1"
                     /translation="MPIITDVYAREVLDSRGNPTVEVEVLTESGAFGRALVPSGASTG
                     EHEAVELRDGDKSRYLGKGVTKAVENVNEIIAPEIIEGEFSVLDQVSIDKMMIALDGT
                     PNKGKLGANAILGVSIAVARAAADLLGQPLYKYLGGFNGKQLPVPMMNIVNGGSHSDA
                     PIAFQEFMILPVGATTFKESLRWGTEIFHNLKSILSKRGLETAVGDEGGFAPKFEGTE
                     DAVETIIQAIEAAGYKPGEEVFLGFDCASSEFYENGVYDYSKFEGEHGAKRTAAEQVD
                     YLEQLVDKYPIITIEDGMDENDWDGWKQLTERIGDRVQLVGDDLFVTNTEILAKGIEN
                     GIGNSILIKVNQIGTLTETFDAIEMAQKAGYTAVVSHRSGETEDTTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDELFETAKYDGIKSFYNLDK"
     gene            15408..15866
                     /locus_tag="O2701_001344"
     CDS             15408..15866
                     /locus_tag="O2701_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293247.1"
                     /translation="MADRTNKEIKTGRFIATASIVFSILLIIHYFVSLDNATAKALLN
                     LTNQNTSDKAIDYILNSFRFTGIMYILAYLAGFITFWNRHTYVWWFMFAVYVSNSLFT
                     LINLSITIQAIKAAHGAYLTLPILIVIIGSVALAIYMLVVSIKRKSTFNR"
     gene            15933..16166
                     /gene="secG"
                     /locus_tag="O2701_001345"
     CDS             15933..16166
                     /gene="secG"
                     /locus_tag="O2701_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCZ4293248.1"
                     /translation="MHTFLIVLLIIDCIALITVVLLQEGKSSGLSGAISGGAEQLFGK
                     QKQRGVDLFLNRLTIILSILFFVLMICISYLGM"
     gene            16282..17022
                     /locus_tag="O2701_001346"
     CDS             16282..17022
                     /locus_tag="O2701_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HCZ4293249.1"
                     /translation="MQIKLPKPFFFEEGKRAVLLLHGFTGNSSDVRQLGRFLQKKGYT
                     SYAPQYEGHAAPPDEILKSSPFVWFKDALDGYDYLVEQGYDEIVVAGLSLGGDFALKL
                     SLNRDVKGIVTMCAPMGGKTEGAIYEGFLEYARNFKKYEGKDQETIDNEMDHFKPTET
                     LKELSEALDTIKEQVDEVLDPILVIQAENDNMIDPQSANYIYDHVDSDDKNIKWYSES
                     GHVITIDKEKEQVFEDIYQFLESLDWSE"
     gene            17056..19428
                     /gene="rnr"
                     /locus_tag="O2701_001347"
     CDS             17056..19428
                     /gene="rnr"
                     /locus_tag="O2701_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCZ4293250.1"
                     /translation="MNLKQSIEEIINQPEYEPMSVSDFQDALGLSSADSFRDLIKVLV
                     ELEQSGLIERTKTDRYQKKHSYRGQSKLIKGTLSQNKKGFAFLRPEDEDMEDIFIPPT
                     KINRALDGDTVIVEIHQSKGEHKGKIEGEVKSIEKHSVTQVVGTYSEARHFGFVIPDD
                     KRIMQDIFIPKGQSLGAVDGHKVLVQITKYADGSDNPEGHISAILGHKNDPGVDILSI
                     IYQHGIEIEFPDEVLQEAEAVPDHIENTEIKGRHDLRDELTITIDGADAKDLDDAISV
                     KKLANGNTQLTVSIADVSYYVTEGSALDKEAYDRATSVYLVDRVIPMIPHRLSNGICS
                     LNPNVDRLTLSCRMEIDASGRVVKHEIFDSVIHSDYRMTYDAVNQIITEKDPNIREQY
                     NEITPMLDLAQDLSNRLIQMRKRRGEIDFDISEAKVLVNEDGIPTDVQLRQRGEGERL
                     IESFMLIANETVAEHFSKLDVPFIYRVHEQPKSDRLRQFFDFITNFGIMIKGTGEDIH
                     PTTLQKVQEEVEGRPEQMVISTMMLRSMQQAHYDDVNLGHFGLSAEYYTHFTSPIRRY
                     PDLTVHRLIRKYLIEKSMDNKEVKRWEDKLPELAEHTSKRERRAIEAERDTDELKKAE
                     YMIQHIGDEFEGIVSSVANFGMFIELPNTIEGMVHIANMTDDYYRFEERQMALIGERQ
                     AKVFRIGDTVKVKVTHVDVDERLIDFQIVGMPLPKNDRSQRPARGKTIQAKTRGKSLD
                     KSKSDDKGRKKKGKQRKGKNQRNNDKSGNSKHKPFYKDKSVKKKARRKKK"
     gene            19450..19914
                     /gene="smpB"
                     /locus_tag="O2701_001348"
     CDS             19450..19914
                     /gene="smpB"
                     /locus_tag="O2701_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCZ4293251.1"
                     /translation="MAKKKSPGTLAENRKARHDYNIEDTIEAGIVLQGTEIKSIRRGS
                     ANLKDSYAQVKNGEMYLNNMHIAPYEEGNRFNHDPLRSRKLLLHKREIIKLGDQTREI
                     GYSIVPLKLYLKHGHCKVLLGVARGKKKYDKRQALKEKAVKRDVARDMKARY"
     gene            20005..20366
                     /gene="ssrA"
                     /locus_tag="O2701_001349"
     tmRNA           20005..20366
                     /gene="ssrA"
                     /locus_tag="O2701_001349"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(21236..21559)
                     /locus_tag="O2701_001350"
     CDS             complement(21236..21559)
                     /locus_tag="O2701_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293252.1"
                     /translation="MNNEELEMRLLLMKQSIEQLQEELAPNLKTRDLVLLRYMYSYKE
                     INMLDSYLFQLATNKEQITKKQFKTKLENIREVPEIPIRQVNDILEGYKNSELYVELI
                     NSILK"
     gene            complement(21575..21828)
                     /locus_tag="O2701_001351"
                     /pseudo
     CDS             complement(21575..21828)
                     /locus_tag="O2701_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015728980.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21913..22641)
                     /locus_tag="O2701_001352"
     CDS             complement(21913..22641)
                     /locus_tag="O2701_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5067 domain-containing protein"
                     /protein_id="HCZ4293253.1"
                     /translation="MKKVMGILLASTLILGACGHHQDSAKKESTSHKKKENDNEELNE
                     ELKEFKSKKNMDIKIKGDTIVSDKFEAKIKEPFIINEKDEKKKYIAFKMEITAKKDDK
                     DLNPSSISHDYINITQDDKNTVNKLRDGYLLSDKKYKDWTEHNQDQIKKGKTAQAMFI
                     YELRGDGNINLNVHKYSEDKTVDSKSFKFSKLKTEDFSHRAETREEVEKKEKEFEEEY
                     KKEQEREKEKEKQKDDDHSGLDEV"
BASE COUNT         7967 a         3382 c         4268 g         7175 t
ORIGIN      
        1 gttgtcccac cccaacttgc acattattgt aagctgactt tccgccagct tctgtgttgg
       61 ggccccgcca acttgcacat tattgtaagc tgacttttcg tcagcttctg tgttggggcc
      121 ccgccaactt gcattgtctg tagaaattgg gaatccaatt tctctatgtt ggggcccaca
      181 ccccaactcg cattgcctgt agaatttctt ttcgaaattc tctgtgttgg ggcccctgac
      241 tagagttgaa aaaagcttgt tgcaagcgca ttttcattca gtcaactact agcaatataa
      301 tattatagac cctaggacat tgatttatgt cccaagctcc ttttaaatga tgtatatttt
      361 tagaaattta atctagacat agttggaaat aaatataaaa catcgttgct taattttgtc
      421 atagaacatt taaattaaca tcatgaaatt cgttttggcg gtgaaaaaat aatggataat
      481 aatgaaaaag aaaaaagtaa aagtgaacta ttagttgtaa caggtttatc tggcgcaggt
      541 aaatctttgg ttattcaatg tttagaagac atgggatatt tttgtgtaga taatctacca
      601 ccagtgttat tgcctaaatt tgtagagttg atggaacaag gaaatccatc cttaagaaaa
      661 gtggcaattg caattgattt aagaggtaag gaactattta attcattagt tgcagtagtg
      721 gataaagtca aaagtgaaag tgacgtcatc attgatgtta tgtttttaga agcaagtact
      781 gaaaaattaa tttcaagata taaggaaacg cgtcgtgcac atcctttgat ggaacaaggt
      841 aaaagatcgt taatcaatgc aattaatgat gagcgagagc atttgtctca aattagaagt
      901 atagctaatt ttgttataga tactacaaag ttatcaccta aagaattaaa agaacgcatt
      961 cgtcgatact atgaagatga agagtttgaa acttttacaa ttaatgttac aagtttcggt
     1021 tttaaacatg ggattcagat ggatgcagat ttagtatttg atgtacgatt tttaccaaat
     1081 ccatattatg tagtagattt aagaccttta acaggattag ataaagacgt ttataattat
     1141 gttatgaaat ggaaagagac ggagattttc tttgaaaaat taactgattt gttagatttt
     1201 atgatacccg ggtataaaaa agaagggaaa tctcaattag taattgccat cggttgtacg
     1261 ggtggacaac atcgatctgt agcattagca gaacgactag gtaattatct aaatgaagta
     1321 tttgaatata atgtttatgt gcatcatagg gacgcacata ttgaaagtgg cgagaaaaaa
     1381 tgagacaaat aaaagttgta cttatcggtg gtggcactgg cttatcagtt atggctaggg
     1441 gattaagaga attcccaatt gatattacgg cgattgtaac agttgctgat aatggtggga
     1501 gtacagggaa aatcagagat gaaatggata taccagcacc aggagacatc agaaatgtga
     1561 ttgcagcttt aagtgattct gagtcagttt taagccaact ttttcagtat cgctttgaag
     1621 aaaatcaaat tagcggtcac tcattaggta atttattaat cgcaggtatg actaatatta
     1681 cgaatgattt cggacatgcc attaaagcat taagtaaaat tttaaatatt aaaggtagag
     1741 tcattccatc tacaaataca agtgtgcaat taaatgctgt tatggaagat ggagaaattg
     1801 tttttggaga aacaaatatt cctaaaaaac ataaaaaaat tgatcgtgtg tttttagaac
     1861 ctaacgatgt gcaaccaatg gaagaagcaa tcgatgcttt aagggaagca gatttaatcg
     1921 ttcttggacc agggtcatta tatacgagcg ttatttctaa cttatgtgtg aatggtattt
     1981 cagatgcgtt aattcattct gatgcgccta agctatatgt ttctaatgtg atgacgcaac
     2041 ctggggaaac agatggttat agcgtgaaag atcatatcga tgcgattcat agacaagctg
     2101 gacaaccgtt tattgattat gtcatttgta gtacacaaac tttcaatgct caagttttga
     2161 aaaaatatga agaaaaacat tctaaaccag ttgaagttaa taaggctgaa cttgaaaaag
     2221 aaagcataaa tgtaaaaaca tcttcaaatt tagttgaaat ttctgaaaat catttagtaa
     2281 gacataatac taaagtgtta tcgacaatga tttatgacat agctttagaa ttaattagta
     2341 ctattccttt cgtaccaagt gataaacgta aataatatag aacgtaatca tattatgata
     2401 tgataataga gctgtgaaaa aaatgaaaat agacagtggt tctaaggtga atcatgtttt
     2461 aaataagaaa ggaatgactg tacgatgagc tttgcatcag aaatgaaaaa tgaattaact
     2521 agaatagacg tcgaggaaat gaatgcaaaa gcagagctca gtgcactgat tcgaatgaat
     2581 ggtgcactta gtctttcaaa tcaacaattt gttataaatg ttcaaacgga aaatgcaaca
     2641 acggcaagac gtatttattc gttgattaaa cgtgtcttta atgtggaagt tgaaatatta
     2701 gtccgtaaaa aaatgaaact taaaaaaaat aatatttata tttgtcgtac aaagatgaaa
     2761 gcgaaagaaa ttcttgatga attaggaatt ttaaaagacg gcatttttac gcatgaaatt
     2821 gatcattcaa tgattcaaga tgacgaaatg agacgcagtt acttgagagg agcttttctg
     2881 gcaggtggct cagtgaataa ccctgaaaca tcttcgtacc atttggaaat tttttctcaa
     2941 aatgagagtc atgcagaagg cttaacgaaa ctaatgaata gttatgagtt gaatgccaaa
     3001 catttagagc gaaaaaaagg aagtattacg tatttaaaag aagcggaaaa gatttcggat
     3061 tttcttagtt tgataggtgg ctatcaagcg ttattaaaat ttgaagacgt acgtattgta
     3121 agagatatgc gtaattctgt taaccgactc gttaattgtg aaacggccaa tctaaataaa
     3181 acagttagtg ctgcgatgaa acaagttgag agcattaaat tgattgataa agaaattggt
     3241 attgaaaatt taccagacag gttgagagag attgctagaa ttcgagtaga acatcaagaa
     3301 atttcgttga aagagcttgg agaaatggta tcaactggtc caatttcaaa atcaggtgta
     3361 aatcaccgat taagaaaact gaatgattta gccgataaga ttagaaatgg tgaacaaata
     3421 gaattataag taagaaggtg tttttggtag tgaattatca aaaacacttt ttttatgatt
     3481 aaaaagtgtt taatgaataa tagtcgacta actattaaat tgagctggaa agtttaatga
     3541 aggaaattca ataaccaagt gatgcctaat tttctcatta aatagcatca tatgtatcaa
     3601 tttggtcata agaaaatatg aatgtgataa ttaattgagt ccctgaaagt ccctgatcat
     3661 agacaaaaac aaaatcagca taaataagaa tcccgacatt gcgggattct ctgtattgaa
     3721 aagtattgta ttttattaaa acagcctcct tgaagggaat tgaaccccta tcttaagaac
     3781 cggaatctta cgtgttatcc attacaccac aaggagcaaa tatgtaatca tatctctgta
     3841 ctatgagaag taaaataagt acaatctaaa atagatatta tcagtttaca aaggaaagag
     3901 aaaagcgtca aacaatgtaa ctatttaaag tcaaagtgtt tgaccaaatt tgacttaata
     3961 tgtaaaataa tgagtaacag ttattacaag gaggaaatat agatgaattt aattcctaca
     4021 gttattgaaa caacaaaccg cggtgaacgt gcatatgata tatactcacg tttattaaaa
     4081 gaccgtatta ttatgttagg ttcacaaatt gatgacaacg tagcaaattc aatcgtatca
     4141 cagttattat tcttacaagc gcaagactca gagaaagata tttatttata cattaattca
     4201 ccaggtggaa gtgtaacagc tggttttgcg atttatgata caattcaaca cattaaacct
     4261 gatgttcaaa caatttgtat cggtatggct gcatcaatgg gatcattctt attagcagct
     4321 ggtgcaaaag gtaaacgttt cgcgttacca aatgcagaag taatgattca ccaaccatta
     4381 ggtggtgctc aaggacaagc aactgaaatc gaaattgctg caaatcacat tttaaaaaca
     4441 cgtgaaaaat taaaccgcat tttatcagag cgtactggtc aaagtattga aaaaatacaa
     4501 aaagacacag atcgtgataa cttcttaact gcagaagaag ctaaagaata tggcttaatt
     4561 gatgaagtga tggtacctga aacaaaataa ttcaaagtaa agagtagact aagctgtctg
     4621 ctctttttgt atgagtaaac caaggtgtca ataatttgtt tactatactt tgagcggaaa
     4681 tatgattgaa tgaagctagt tgaaccgtaa ctatatgaaa tgttcccttc aaagtagaca
     4741 ttgaaaggaa catttcaatc ctttgtttgt aagtcgctct agacattaca tttagtacat
     4801 atgttgtttc taatgctcat taatggtatt gattattctt taattaaatc ttcaagtgcc
     4861 atttttaaat tactatattt aaattggaat cccaatgctt gaattttatt aggtaatact
     4921 ttttgagtat ccaatactac tgttgacatt tgaccaagta tgagacgcat tgcaagactt
     4981 ggtgcccaag tttcatgagg cttatgcata gctcttgcta aagtgtagcc aaataaattt
     5041 tgacgttcag gtataggtgc agttaaatta aacggaccac tagctgactc gttatttatt
     5101 aaaaataaaa tagcttgaat taaatcattg atatgaatcc atgaatacca ttgttgacca
     5161 gaacctaatt taccaccaat gtaatattcg tatggtagtt tcattgtttg taacgcaccg
     5221 ccttcattcg ataaaattat accgaaacga ccgatgacaa ctcgtgtacc taattgttca
     5281 aattgttgtg cgaaacgttc ccattgatac acaatatctg ataagaaatc aaaaggtaaa
     5341 gttttataaa cttctgtgta actcataaat aaatcaggag gatagtaacc agtggcacta
     5401 gcattaaata aagctttagg tgctttatta cgtgatttaa acaattcata taaagcttgc
     5461 gtagattgaa ttctacttag cattagcgtt tgtttatatt ccggtgtcca tcgtttattc
     5521 aatgtagcac ctgctaagtt gatgaccaca tcgatatttt gaggaacttt gtgttcccac
     5581 ccagatttag cccagttgac atatgaaatt ttcttatcat ttgaaatttg gtcgtgtcgc
     5641 gttaatatcg tgatatgtga atctgatttt ttaatttcat taactaattg agatccaacc
     5701 ataccagtcc caccagtaat taagtattgt ttcattatca ttcaccccat gtaattttgt
     5761 atttagttac tatttaataa tcattattgt tgttcaaagg ttatacatta tttagacaat
     5821 aatatgtcaa taactttttt tgaattgtat tttaataaaa atgatataag ttataataaa
     5881 ggtgtacctt cgataacgaa taaacatctc ttaaaagtat gtgtaaaacg ctgcatgata
     5941 caaacgaagg taaaaatttg actcccttta gtagtggacc cgtacgttaa tcgtgcgggt
     6001 cgtttttatt tatttcttat tcccattata catcaattta aagcattaat tttttaaaca
     6061 aatttaagaa tacatagtaa tataacaatc taaacataaa aacttttaac acaacactta
     6121 aaccaatgct ttaattttca atacgtagct ataatttgtt gtaaaatcaa aaaggttaaa
     6181 atgttaattt tcaaaaaagg ctcaaaatat gtttgattta gttattaaat gttaagatat
     6241 ataagactac tatttctttg taaaaatgaa tccgatttac gagtgagtaa tagtgaaggc
     6301 agttttaagt tgaagaaggc aaaaagagta aatgtttatt aatatttgta gaaactaggt
     6361 aagcaaatta gttgtgaaaa tgttaatggt tgcgtgataa tttctatatt taaattagtt
     6421 tgaagtgagg gagagtatgt cgaatcaaaa ttacgactac aataaaaatg aagatggaag
     6481 taagaagaaa atgagtacaa cagcgaaagt agttagcatt gcgacggtat tgctattact
     6541 cggaggatta gtatttgcaa tttttgcata tgtagatcat tcgaataaag ctaaagaacg
     6601 tatgttgaac gaacaaaagc aggaacaaaa agaaaagcgt caaaaagaaa atgcagaaaa
     6661 agagagaaag aaaaagcaac aagaggaaaa agagcagaat gagctagatt cacaagcaaa
     6721 ccaatatcag caattgccac agcagaatca atatcaatat gtgccacctc agcaacaagc
     6781 acctacaaag caacgtcctg ctaaagaaga gaatgatgat aaagcatcaa aggatgagtc
     6841 gaaagataag gatgacaaag catctcaaga taaatcagat gataatcaga agaaaactga
     6901 tgataataaa caaccagctc agcctaaacc acagccgcaa caaccaacac caaagccaaa
     6961 taataatcaa caaaacaatc aatcaaatca gcaagcaaaa ccacaagcac cacaacaaaa
     7021 tagccaatca acaacaaata aacaaaataa tgctaatgat aagtagtatt tagtcaaaca
     7081 aaaatgaacc agtatgacag acaacacaat taattaggtt gtctcgaata ttggttctta
     7141 tttttataat tgttaattag gggagagatg atacttaaat agttagttat ttattttacg
     7201 gatagtgaaa tttattttga gtgaggtggg acagaaatga tattttcgca aaatttattt
     7261 cgtcgtccca ccccaacttc cattgcctgt agaaattgga aatccaattt ctctatgttg
     7321 gggccccacc ccaacttgca ttgcctgtag aaattgggag gagccaattt cactatgttg
     7381 gggccccacc ccaactcgca ttgcctgtag aatttcattt cgaaattctc tatgttgggg
     7441 cccctgactt taattgaaaa aagcttgtta caagtgcatt ttcgttcggt taactactac
     7501 taatgtgact ttttggattc tagagcattg atttatgtcc tagtctcaaa taataagcaa
     7561 atgattatcg agtcagtata agggtataca tttgactaca gcgaataaaa taaaacgttt
     7621 tgttgaataa caaatcgaaa atatattgca agcgctttat caaattattt agaaaattta
     7681 agttttatgc ttgcaatttt tgaaatagaa tagtactatt gcaagtgtaa agaggttaat
     7741 ttttgtccca cgcgggactt aaaaaggcaa ccactggttg tgacatatcc ttatttacat
     7801 ttataaatat aaggaggagg tagtagtgaa agacttattg caagcacagc aaaagcttat
     7861 accggatctc atagataaaa tgtataaacg tttttctatt cttactacta tctcaaaaaa
     7921 tcagcctgtc ggacgtcgaa gtttaagcga acatatggat atgactgaac gtgtactgcg
     7981 ttctgaaaca gatatgctta agaaacaaga tttgataaaa gttaagccta ccggaatgga
     8041 aattacagct gaaggtgagc aactgatttc gcaattgaaa ggttactttg atatctatgc
     8101 agatgataat cgtctgtcag aaggtattaa gaataaattt caaattaagg aagttcatgt
     8161 tgttcctggt gatgctgata atagtcaatc tgttaaaaca gaattaggta gacaagcagg
     8221 tcaattactt gaaggcatat tacaagaaga cgcgatagtt gctgtaactg gcggatccac
     8281 gatggcatgt gttagtgaag caattcattt attaccatat aatgtattct tcgtaccagc
     8341 cagaggtgga ctaggcgaaa atgttgtctt tcaggcaaac acaattgcag ccagtatggc
     8401 acaacaagct ggcggttatt atacgacgat gtatgtacct gataatgtca gtgaaacaac
     8461 atataataca ttgttgttag agccatcagt cataaacact ttagacaaaa ttaaacaagc
     8521 aaacgttata ttacacggca ttggtgatgc gctgaagatg gcgcatcgac gtcaatcacc
     8581 tgaaaaggtc attgaacaac ttcaacatca tcaagctgtc ggagaggcat ttggttatta
     8641 ttttgataca caaggtcaaa ttgtccataa ggttaaaaca attggacttc aattagaaga
     8701 ccttgaatca aaagacttta tttttgcagt tgcaggaggc aaatcgaaag gtgaagcaat
     8761 taaagcatac ttgacgattg cacccaagaa tacagtgtta atcactgatg aagccgcagc
     8821 aaagataata cttgaataag agataaaaag tttaatactt tttaaatatc attttaaagg
     8881 aggccattat aatggcagta aaagtagcaa ttaatggttt tggtagaatt ggtcgtttag
     8941 cattcagaag aattcaagaa gtagaaggtc ttgaagttgt agcagtaaac gacttaacag
     9001 atgacgacat gttagcgcat ttattaaaat atgacactat gcaaggtcgt ttcacaggtg
     9061 aagtagaggt agttgatggt ggtttccgcg taaatggtaa agaagttaaa tcattcagtg
     9121 aaccagatgc aagcaaatta ccttggaaag acttaaatat cgatgtagta ttagaatgta
     9181 ctggtttcta cactgataaa gataaagcac aagctcatat tgaagcaggc gctaaaaaag
     9241 tattaatctc agcaccagct actggtgact taaaaacaat cgtattcaac actaaccacc
     9301 aagagttaga cggttctgaa acagttgttt caggtgcttc atgtactaca aactcattag
     9361 caccagttgc taaagtttta aacgatgact ttggtttagt tgaaggttta atgactacaa
     9421 ttcacgctta cacaggtgat caaaatacac aagacgcacc tcacagaaaa ggtgacaaac
     9481 gtcgtgctcg tgcagcggca gaaaacatca tccctaactc aacaggtgct gctaaagcta
     9541 tcggtaaagt tattcctgaa atcgatggta aattagatgg tggtgcacaa cgtgttcctg
     9601 tagctacagg ttcattaact gaattaacag tagtattaga aaaacaagac gtaacagttg
     9661 aacaagttaa cgaagctatg aaaaatgctt caaacgaatc attcggttac actgaagacg
     9721 aaatcgtttc ttcagacgtt gtaggtatga cttacggttc attattcgac gctacacaaa
     9781 ctcgtgtaat gtcagttggc gaccgtcaat tagttaaagt tgcagcttgg tatgataacg
     9841 aaatgtcata tactgcacaa ttagttcgta cattagcata cttagctgaa ctttctaaat
     9901 aattttagta tagtttttat tcaaatacgc tagtgctcag aactatttag cattaattaa
     9961 agcttatgag taagcgggga gcacaaacgc ttctccgctt atttttatat aaaatttcct
    10021 aattacaagg aggaaacacc atggctaaaa aaattgtttc tgatttagat cttaaaggta
    10081 aaacagtcct agtacgtgct gattttaacg tacctttaaa agacggtgaa attactaatg
    10141 acaaccgtat cgttcaagct ttacctacaa ttcaatacat catcgaacaa ggtggtaaaa
    10201 tcgtactatt ttcacattta ggtaaagtga aagaagaaag tgataaagca aaattaactt
    10261 tacgtccagt tgctgaagac ttatctaaga aattagataa agaagttgtt ttcgtaccag
    10321 aaacacgcgg cgaaaaactt gaagctgcta ttaaagacct taaagaaggc gacgtattat
    10381 tagttgaaaa tacacgttat gaagatttag acggtaaaaa agaatctaaa aatgatccag
    10441 aattaggtaa atactgggca tctttaggtg atgtgtttgt aaatgatgct tttggtactg
    10501 cgcatcgtga gcatgcatct aatgttggta tttctacaca tttagaaact gcagctggat
    10561 tcttaatgga taaagaaatt aagtttattg gcggcgtagt taacgatcca cataaaccag
    10621 ttgttgctat tttaggtgga gcaaaagtat ctgacaaaat taatgtcatc aaaaacttag
    10681 ttaacatagc tgataaaatt atcatcggcg gaggtatggc ttatactttc ttaaaagcgc
    10741 aaggtaaaga aattggtatt tcattactag aagaagataa aatcgacttc gcaaaagatt
    10801 tattagaaaa acatggtgat aaaattgtat taccagtaga cactaaagtt gctaaagaat
    10861 tttctaatga tgccaaaatc actgtagtac catctgattc aattccagca gaccaagaag
    10921 gtatggatat tggaccaaac actgtaaaat tatttgcaga tgaattagaa ggtgcgcaca
    10981 ctgttgtatg gaatggacct atgggtgtat tcgagttcag taactttgca caaggtacaa
    11041 ttggtgtatg taaagcaatt gcaaacctta aagatgcaat tacgattatc ggtggcggtg
    11101 attcagctgc agcagcaatc tctttaggtt ttgaaaatga cttcactcat atttcaactg
    11161 gtggcggcgc gtcattagag tacctagaag gtaaagaatt gcctggtatc aaagcaatca
    11221 ataataaata ataaagtgat agtttaaagt gatgtggcat gtttgtttaa cattgttacg
    11281 ggaaaacagt cacaagatga catcgtgttt catcactttt caaaaatatt tacaaaacaa
    11341 ggagtgtctt taatgagaac accaattata gctggtaact ggaaaatgaa caaaacagta
    11401 caagaagcaa aagacttcgt caatgcatta ccaacattac cagattcaaa agaagtagaa
    11461 tcagtaattt gtgcaccagc aattcaatta gatgcattaa ctactgcagt taaagaagga
    11521 aaagcacaag gtttagaaat cggtgctcaa aatacgtatt tcgaagataa tggtgcgttc
    11581 acaggtgaaa cgtctccagt tgcattagca gatttaggcg ttaaatacgt tgttatcggt
    11641 cattctgaac gtcgtgaatt attccacgaa acagatgaag aaattaacaa aaaagcgcac
    11701 gctattttca aacatggaat gactccaatt atatgtgttg gtgaaacaga cgaagagcgt
    11761 gaaagtggta aagctaacga tgttgtaggt gagcaagtta agaaagctgt tgcaggttta
    11821 tctgaagatc aacttaaatc agttgtaatt gcttatgaac caatctgggc aatcggaact
    11881 ggtaaatcat caacatctga agatgcaaat gaaatgtgtg catttgtacg tcaaactatt
    11941 gctgacttat caagcaaaga agtatcagaa gcaactcgta ttcaatatgg tggtagtgtt
    12001 aaacctaaca acattaaaga atacatggca caaactgata ttgatggggc attagtaggt
    12061 ggcgcatcac ttaaagttga agatttcgta caattgttag aaggtgcaaa ataatcatgg
    12121 ctaagaaacc aactgcgtta attattttag atggttttgc gaaccgcgaa agcgaacatg
    12181 gtaatgcggt aaaattagca aacaagccta attttgatcg ttattacaac aaatatccaa
    12241 cgactcaaat cgaagcgagt ggcttagatg ttggactacc tgaaggacaa atgggtaact
    12301 cagaagttgg tcatatgaat atcggtgcag gacgtatcgt ttatcaaagt ttaactcgaa
    12361 tcaataaatc aattgaagac ggtgatttct ttgaaaatga tgttttaaat aatgcaattg
    12421 cacacgtgaa ttcacatgat tcagcgttac acatctttgg tttattgtct gacggtggtg
    12481 tacacagtca ttacaaacat ttatttgctt tgttagaact tgctaaaaaa caaggtgttg
    12541 aaaaagttta cgtacacgca tttttagatg gccgtgacgt agatcaaaaa tccgctttga
    12601 aatacatcga agagactgaa gctaaattca atgaattagg cattggtcaa tttgcatctg
    12661 tgtctggtcg ttattatgca atggatcgtg acaaacgttg ggaacgtgaa gaaaaagctt
    12721 acaatgctat tcgtaatttt gatgccccaa cttatgcaac tgccaaagaa ggtgtagaag
    12781 caagctataa tgagggctta actgacgaat tcgtagtacc attcatcgtt gagaatcaaa
    12841 atgacggtgt taatgatgga gatgcagtga tcttctataa tttccgacct gatagagcag
    12901 cgcaattatc ggaaattttt gcgaacagag cattcgaagg ctttaaagtt gaacaagtta
    12961 aagacttatt ctatgcaaca ttcactaagt ataatgacaa tatcgatgcg gctatcgtct
    13021 tcgaaaaagt tgatttaaat aatacaattg gtgaaattgc acaaaataac aatttaactc
    13081 aattacgtat tgcagaaact gaaaaatacc ctcacgttac ttactttatg agtggtggac
    13141 gtaacgagga atttaaaggt gaacgccgtc gtttaattga ttcacctaaa gttgcaacgt
    13201 atgacttgaa accagaaatg agtgcttatg aagttaaaga tgcattatta gaagagttaa
    13261 ataaaggtga cttggactta attattttaa actttgctaa ccctgatatg gttggacata
    13321 gtggtatgct tgagccgaca atcaaagcaa tcgaagcggt tgatgaatgt ttaggagaag
    13381 tggttgataa gattttagac atggacggtt atgcaattat tactgctgac catggtaact
    13441 ctgatcaagt attgacggat gatgatcaac caatgactac gcatacaacg aacccagtac
    13501 cagtgattgt aacaaaagaa ggcgttacac ttagagaaac tggtcgctta ggtgacttag
    13561 cacctacatt attagattta ttaaatgtag aacaacctga agatatgaca ggtgaatctt
    13621 taattaaaca ctaatattgt aaaagatgtt aagtaaacgc ttaatgacac ttattttttg
    13681 aaaataatag taatatcatt ttgttaaatg aaagaataaa gctataataa ttatagaata
    13741 actatttaaa ggagattata aacatgccaa ttattacaga tgtttacgct cgcgaagtct
    13801 tagactctcg tggtaaccca actgttgaag tagaagtatt aactgaaagt ggcgcatttg
    13861 gtcgtgcatt agtaccatca ggtgcttcaa ctggtgaaca cgaagctgtt gaattacgtg
    13921 atggagacaa atcacgttat ttaggtaaag gtgttactaa agcagttgaa aacgttaatg
    13981 aaatcatcgc accagaaatt attgaaggtg aattttcagt attagatcaa gtatctattg
    14041 ataaaatgat gatcgcatta gacggtactc caaacaaagg taaattaggt gcaaatgcta
    14101 ttttaggtgt atctatcgca gtagcacgtg cagcagctga cttattaggt caaccacttt
    14161 acaaatattt aggtggattt aatggtaagc agttaccagt accaatgatg aacatcgtta
    14221 atggtggttc tcactcagat gctccaattg cattccaaga attcatgatt ttacctgtag
    14281 gtgctacaac gttcaaagaa tcattacgtt ggggtactga aattttccac aacttaaaat
    14341 caattttaag caaacgtggt ttagaaactg cagtaggtga cgaaggtggt ttcgctccta
    14401 aatttgaagg tactgaagat gctgttgaaa caattatcca agcaatcgaa gcagctggtt
    14461 acaaaccagg tgaagaagta ttcttaggat ttgactgtgc atcatcagaa ttctatgaaa
    14521 atggtgtata tgactacagt aagttcgaag gcgaacacgg tgcaaaacgt acagctgcag
    14581 aacaagttga ctacttagaa caattagtag acaaatatcc tatcattaca attgaagacg
    14641 gtatggacga aaacgactgg gatggttgga aacaacttac agaacgtatc ggtgaccgtg
    14701 tacaattagt aggtgacgat ttattcgtaa caaacactga aattttagca aaaggtattg
    14761 aaaacggaat tggtaactca atcttaatta aagttaacca aatcggtaca ttaactgaaa
    14821 catttgatgc aatcgaaatg gctcaaaaag ctggttacac agcagtagtt tctcaccgtt
    14881 caggtgaaac agaagataca acaattgctg atattgctgt tgctacaaac gctggtcaaa
    14941 ttaaaactgg ttcattatca cgtactgacc gtattgctaa atacaatcaa ttattacgta
    15001 tcgaagatga attatttgaa actgctaaat atgacggtat caaatcattc tataacttag
    15061 ataaataatt ttctttataa tcaaatgctg acataatttt agttgaggat tattatgacg
    15121 gtataaatta aataaagatt ttgagttcac gcttaaataa gttcacgctt aaatttatag
    15181 cctgccacag agttgagact gtggtaggtt ttttattttg aagtattaat cataacagac
    15241 taataatcat gaggtaacta ataacacata tttaacttgt attcttaaac tggtataata
    15301 aatttatgtt gaaatgaata ttgtatgaca gggtattcac ttttattaaa aggtaaaatt
    15361 aaataaaggt tttatagaac gtatttaaat atatgaggag taaacaaatg gctgatagaa
    15421 cgaataaaga aattaaaaca ggacgcttta ttgcaactgc atcaatcgta ttctcaatat
    15481 tattgattat tcattacttt gtttcgttgg ataatgcgac tgccaaagca ttacttaatt
    15541 taacgaatca aaacacttca gataaagcga ttgattacat tttaaacagc tttagattca
    15601 ctggtattat gtatattttg gcttatctag caggcttcat cactttttgg aatcgacata
    15661 cttatgtgtg gtggtttatg tttgcagttt atgtatcaaa tagtttgttt acgttgatta
    15721 atttatcaat cacaattcaa gcaataaaag ctgcacacgg tgcgtactta acattgccaa
    15781 ttttaattgt tattataggt tcggttgcat tagcgattta tatgcttgtt gtttctatca
    15841 aacgtaaaag tacatttaat cgctagaaaa ttgattttaa caataaaaat atgatatact
    15901 acttgtcgta tataaggaac ggaggacaat ttatgcatac atttttaatc gtattattaa
    15961 tcattgattg tattgcatta ataactgttg tactactcca agaaggtaaa agcagtggac
    16021 tttcaggtgc catcagtggt ggtgctgagc agttattcgg taaacaaaaa caacgtggcg
    16081 tcgatttatt cttaaataga ttaacaatta ttttatcaat attatttttt gtacttatga
    16141 tttgcataag ttatcttggt atgtaaggtc cggcgatgta aatgtcgggc ttttttattt
    16201 ataattaaga atgtaatagt ttaacaataa gctatgtaaa atatatagcc tagttaagta
    16261 tgcaaaggga gcgttagatt tatgcagata aaattaccaa aacctttctt ttttgaggaa
    16321 ggtaaacgtg ccgtgttatt actacatggt tttacaggca attcgtctga tgttcgtcaa
    16381 ttaggtcgat ttttacaaaa gaaaggttat acatcatatg caccgcaata tgaaggccac
    16441 gcggcaccac cagatgaaat actgaaatct agtcctttcg tttggtttaa agatgcgtta
    16501 gatggttatg attatcttgt tgaacaaggt tatgatgaaa ttgttgttgc tggtctatca
    16561 ttaggtgggg attttgcttt aaaattaagc ttaaatagag atgtaaaggg tattgtaacg
    16621 atgtgtgctc ctatgggtgg caaaactgaa ggtgccattt atgaaggctt tttagaatat
    16681 gcacgcaatt ttaaaaagta tgaaggtaaa gatcaagaga ctattgataa tgaaatggat
    16741 cattttaaac caactgaaac tttaaaagaa ctaagtgaag cattagatac gattaaagag
    16801 caagttgatg aagtgttgga tcctatttta gtgattcaag cagaaaacga caatatgatt
    16861 gatccacaat ccgcaaatta tatatatgac catgtagatt ctgatgacaa aaatatcaag
    16921 tggtacagtg aatctggaca tgttattacg attgataaag agaaagaaca agtatttgaa
    16981 gatatttatc aatttttaga gtcattagac tggtcagaat aaaaagagat tttaacatta
    17041 gaaaggaggg gcataatgaa tttaaagcaa tctatagaag agattattaa tcaacctgaa
    17101 tatgaaccta tgtcagtgtc agattttcaa gatgcattag gtttaagcag tgccgactcg
    17161 tttagagatt taattaaggt gcttgtggag ttagaacaat caggattaat cgaacgtaca
    17221 aaaacagaca gataccaaaa aaagcatagt tatagaggtc aatcaaaatt gataaaagga
    17281 acgttaagtc aaaataaaaa aggctttgca ttcttaagac ctgaagatga ggatatggaa
    17341 gatatattta ttcccccgac gaaaattaat cgtgccttgg atggagatac tgttattgta
    17401 gaaatccatc aatcaaaagg tgaacataaa ggtaaaatcg aaggggaagt taagtcgatt
    17461 gagaagcatt ctgtaactca agttgttggt acgtatagtg aagctagaca ttttggcttt
    17521 gttattccgg atgataaacg tattatgcaa gatattttca ttcctaaagg tcaaagttta
    17581 ggcgcagtcg atggtcataa ggtacttgta caaattacta agtatgctga tggttcagat
    17641 aatccagaag gacatatttc tgctatttta ggacataaaa atgatcctgg cgtagatatt
    17701 ttatctatta tctatcaaca tggcatagaa attgaatttc ctgatgaagt gttacaagaa
    17761 gctgaagcag tacctgatca tattgaaaat actgaaatta aaggccgtca tgatttacgt
    17821 gatgaattga caatcacaat tgatggtgct gatgctaaag acttagatga cgcaattagt
    17881 gttaaaaagt tagcgaacgg taatacgcaa ttaactgtaa gtattgctga tgtcagctat
    17941 tatgtaacag aaggttctgc attggataaa gaggcatatg atagagcgac aagtgtatat
    18001 cttgttgacc gtgtaattcc aatgattcca catcgattaa gtaatggtat ttgttcattg
    18061 aatcctaatg ttgatcgttt aactctaagc tgtcgcatgg aaatcgatgc tagtggtcgc
    18121 gttgttaaac atgaaatttt tgatagtgtt atacattctg attatcgaat gacgtatgat
    18181 gcggtaaatc agattattac tgaaaaggat cctaacattc gcgaacaata taatgaaatt
    18241 acgcctatgc tagatttagc acaagattta tctaatcgtt tgattcaaat gagaaaacga
    18301 cgtggtgaaa tcgattttga tattagtgaa gcaaaagtat tagttaacga agacggtata
    18361 ccaacagatg ttcaattaag acaacgtggc gagggtgaac gtctaattga atcatttatg
    18421 ttaattgcaa atgaaacagt tgctgaacat tttagtaagt tagatgtacc ttttatttac
    18481 cgagtgcatg agcaacctaa atcagatcgc ttaagacaat tctttgattt tattacaaac
    18541 tttggcatca tgattaaggg tactggcgaa gatattcatc caacaacact tcaaaaggtt
    18601 caagaagaag tagaaggtcg acctgaacaa atggtcattt caacaatgat gttgcgttca
    18661 atgcaacaag cgcattatga tgatgtgaac ttgggacatt ttggcttatc agctgaatat
    18721 tatacgcatt ttacatcacc aattagacgt tatcctgatt taacagttca tcgtttaatc
    18781 cgtaagtatt taattgagaa atcaatggat aacaaagaag tgaagcgttg ggaagacaaa
    18841 ttgcctgagt tagctgaaca tacttctaaa cgtgaacgtc gtgctattga ggcagaacgt
    18901 gatactgatg aattgaaaaa agcagaatat atgattcaac atattggtga tgaatttgaa
    18961 ggtattgtca gctcagtagc taacttcggt atgttcattg aattgccaaa tacgatagaa
    19021 ggtatggttc atattgcgaa tatgactgat gattattacc gttttgaaga gcgtcaaatg
    19081 gcattaattg gtgagcgtca agctaaagta tttagaattg gtgacacagt taaggttaaa
    19141 gtgacgcatg ttgatgtaga tgaacgatta attgattttc aaattgtagg tatgccttta
    19201 ccgaaaaatg atcgatcaca gcgcccagcg cgaggtaaga caattcaagc caaaacgcgt
    19261 ggtaaatcat tagataaatc aaaatctgat gataagggtc gtaagaaaaa aggtaagcaa
    19321 cgtaaaggta aaaaccaacg taataatgat aaatcaggta atagtaagca taagccattt
    19381 tataaagata aaagtgtgaa aaagaaagca cgtcgtaaga aaaaataagc agcaatgagg
    19441 tgagtatgaa tggctaagaa gaaatcacca ggtacattag cggaaaatcg taaggcaaga
    19501 catgattata atattgaaga tacgattgaa gcgggaattg tattgcaagg cacagaaata
    19561 aaatcaattc gccgaggtag tgctaacctt aaagatagtt atgcgcaagt taaaaacggt
    19621 gaaatgtatt tgaataatat gcatatagca ccatacgaag aagggaatcg ttttaatcac
    19681 gatcctcttc gttctcgaaa attattattg cacaagcgtg aaatcattaa attgggtgat
    19741 caaacacgtg agattggtta ttcgattgtg ccgttaaagc tttatttgaa gcatggacat
    19801 tgtaaagtat tacttggtgt tgcacgaggt aagaaaaaat atgataaacg tcaagctttg
    19861 aaagaaaaag cagtcaaacg agatgttgcg cgcgatatga aagcccgtta ttaagcgatt
    19921 tagttgctta atcgggctat atttgatata gttatatgtg cttttgtaaa ttacaaaagt
    19981 atgatttgtt tgatttatta tttcggggac gttcatggat tcgacagggg tcccccgagc
    20041 tcattaagcg tgtcggaggg ttgtcttcgt catcaacaca cacagtttat aataactggc
    20101 aaatcaaaca ataatttcgc agtagctgcc taatcgcact ctgcatcgcc taacagcatt
    20161 tcctatgtgc tgttaacgcg attcaacctt aataggatat gctaaacact gccgtttgaa
    20221 gtctgtttag aagaaactta atcaaactag catcatgttg gttgtttatc acttttcatg
    20281 atgcgaaacc tatcgataaa ctacacacgt agaaagatgt gtatcaggac ctttggacgc
    20341 gggttcaaat cccgccgtct ccatatttgt agcctacagc ctttgtggtt gtgggctttt
    20401 ttattttgtg tttttcaggg gataatgcat tgcagaattt gttgtgagta ttgatatagc
    20461 agtgtttgta taggtgttta tttgatggag gaaagagtaa taagtgatta tgaattagtt
    20521 tttgagatat aaggggacag tgatgtgtgt caaataagtg tcaaaaaagt tggattctga
    20581 gttttacatt caacattgtt catgaagaaa cttctttata cgcaaaaaat tctccatgtt
    20641 atatatgtca atataaaaat gtgaatcgtc tacacttaat tggataaatg gctactgaaa
    20701 aagaactttt catttttgtt acgtcactaa gtgggtgtag ttataaagag atgagccgag
    20761 ttttgatatt ttcattagaa tcaatatgcc tattaacaca atcagcaata gttgacgaga
    20821 cggaaataaa agaagtcgta gttaagaaat gcatttcaca acataccatt gtagccattt
    20881 ttattgtttt ggatgataaa ctctttttgg aatttttagt ttttataatt tgcaactaca
    20941 ctacttcttt tactaatatt aatgtctaag taatcgataa aaaattttcc attgaataaa
    21001 tgagaagtta aaaactttac ttaacctttc tcattgcatt ttcctattca cgattttaag
    21061 aacccaacat actacaaacg aattttaaaa ggcgagagta aagcttactt gtttattata
    21121 catatttaaa atccaagagt cagaacagac tactcctctt tataactata aaaaatagct
    21181 atgaaaaaat ctatcgtcat agattccttc atagctaatc ttagtatgtt tatttttatt
    21241 ttaggatgct atttatcaac tcaacatata actcactatt tttataacct tctaatatat
    21301 cattaacttg tctaataggt atttctggta cttctctaat gttttccaat tttgttttaa
    21361 attgtttttt tgttatttgc tctttatttg tagccaattg gaacaagtaa gaatctagca
    21421 tattaatttc tttatatgaa tacatatatc ttaataacac taaatctcta gtttttaagt
    21481 taggcgctag ttcttcttgt aattgttcta ttgattgttt cattaataac aatctcattt
    21541 ctaattcttc attattcatt ttatcacact ctttttatat taatgcttga ccaacttggg
    21601 aaacccaaaa ccctatgctt cttgcagtag aatctttaat accagttccc atcaatgctt
    21661 gtgaaacttg accttgtaca tttccccatg tagcctcttc ttgttttaat gcattattca
    21721 atgcgggatt tacaaattta tcccatcttt tttttatgat tttccggcac ggggactgat
    21781 ttctttaaca ccattaaaca cagatttttt atttttaatc atagctttat agtatcatgt
    21841 tggctaagct ataaataagt cagtttctct aaaaattaaa taactgaatg taagacaatc
    21901 aacaaaccaa atttatactt catctaaacc actgtggtcg tcatcttttt gcttttcttt
    21961 ttctttctct cgttcttgtt cttttttgta ctcttcttca aattcttttt ctttcttttc
    22021 tacttcttct cttgtttccg ctctatgaga aaaatcttcg gttttaagtt tactaaattt
    22081 gaatgattta gaatcaactg ttttatcttc tgagtattta tggacattta aattaatatt
    22141 tccatcacct cttaactcat agataaacat ggcttgtgca gttttgcctt ttttaatttg
    22201 atcttggtta tgttctgtcc aatctttata ttttttatca cttaaaagat aaccatctct
    22261 taatttattt actgtatttt tatcatcttg agtgatatta atatagtcat gagaaataga
    22321 agatggattt aaatctttat cgtctttttt agcagtaatt tccattttaa aagcgatata
    22381 tttctttttc tcatcttttt cattgatgat aaacggttct tttattttag cttcaaattt
    22441 gtcactaaca atagtatcgc ctttaatttt tatatccata ttttttttgc ttttaaattc
    22501 tttaagttct tcatttaatt cttcattgtc attttctttc tttttgtgac tagtgctctc
    22561 tttttttgca ctatcttgat gatgtccaca agcacctaag ataagtgtac ttgctaataa
    22621 tatccccatt acttttttca tttaacatgt ctcctttatt tcgcaaaaat ttattttaaa
    22681 aactctaaat gacttatcat tttgagtaat taaacaaagt tgatattttg tgagattcta
    22741 agatgatatt aaataattct tgtaataatg atcctatgta ttgttgcaat aa
//
LOCUS       DALAGD010000044        22777 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.044, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000044 DALAGD010000000
VERSION     DALAGD010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22777
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.044"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            220..2292
                     /gene="lip2"
                     /locus_tag="O2701_001353"
     CDS             220..2292
                     /gene="lip2"
                     /locus_tag="O2701_001353"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip2/Geh"
                     /protein_id="HCZ4293254.1"
                     /translation="MLRGQEERKYSIRKYSIGVVSVLAATMFVVSSHEAQASEKTSTN
                     AAAQKETLNQPGEQGNAITSHQMQSGKQLDDMHKENGKSGTVTEGKDTLQSSKHQSTQ
                     NSKTIRMQNDNQVKQDSERQGSKQSHQNNATNNTERQNDQVQNTHHAERNGSQSTTSQ
                     SNDVDKSQPSIPAQKVIPNHDKAAPTSTTPPSNDKTAPKSTKAQDATTDKHPNQQDTH
                     QPAHQIIDAKQDDTVRQSEQKPQVGDLSKHIDGQNSPEKPTDKNTDNKQLIKDALQAP
                     KTRSTTNAAADAKKVRPLKANQVQPLNKYPVVFVHGFLGLVGDNAPALYPNYWGGNKF
                     KVIEELRKQGYNVHQASVSAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKT
                     YKGIMPNWEPGKKVHLVGHSMGGQTIRLMEEFLRNGNKEEIAYHKAHGGEISPLFTGG
                     HNNMVASITTLATPHNGSQAADKFGNTEAVRKIMFALNRFMGNKYSNIDLGLTQWGFK
                     QLPNESYIDYIKRVSKSKIWTSDDNAAYDLTLDGSAKLNNMTSMNPNITYTTYTGVSS
                     HTGPLGYENPDLGTFFLMATTSRIIGHDAREEWRKNDGVVPVISSLHPSNQPFVNVTN
                     DEPATRRGIWQVKPIIQGWDHVDFIGVDFLDFKRKGAELANFYTGIINDLLRVEATES
                     KGTQLKAS"
     gene            complement(2534..3361)
                     /locus_tag="O2701_001354"
     CDS             complement(2534..3361)
                     /locus_tag="O2701_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCZ4293255.1"
                     /translation="MTEIKDKVITKDAFALPYTIIKAKNQPTKGAIVYIHGGGLMFGK
                     ANDLSPQYIDILTEHYDLIQLSYRLLPEVSLDCIIEDVYASFDAIQSQYSNCPIFTFG
                     RSSGAYLSLLIARDRDIDGVIDFYGYSRINTEPFKTTNSYYAKIAQSVNETMIAQLTS
                     PTPVVQDQIAQRFLIYVYARGTGKWINMINIADYTNSKYNIAPDELKTLPPVFIAHCN
                     GDYDVPVEESEHIMNHVPHSTFERVNKNEHDFDRRPNDEAITIYRKVVDFLNAITMM"
     gene            complement(3434..4633)
                     /locus_tag="O2701_001355"
     CDS             complement(3434..4633)
                     /locus_tag="O2701_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCZ4293256.1"
                     /translation="MYRYKPLLQSIHLPNGIKISNRFVLSPMTVNTSTKEGYITKEDL
                     AYAARRSNSAGMQVTGAAYIEPYGQLFEYGFNIDHDACIPGLTNMASTMKQHGSLAII
                     QLAHAGRFSNQAILNFGKVYGPSPMTLHSPIEHDVIAMSHEKINSIIQQYRDATLRAI
                     KAGFDGVEISIAQRLLIQTFFSTFSNKRTDHYGADSLKNRARLCLEVMRAVQEVIDKE
                     APDNFILGFRATPEETRGSDLGYTIDEFNQLINWVMDVSNIQYLAIASWGRHIYQNTS
                     RTPGDHFGRPVNQIVYEHLAGRIPLIASGGINSPESALDALQHADMVGMSSPFVTEPD
                     FVHKLAEQRPHDINLEFSMADLEDLAIPHAAFKDIVKMMDYGEGLKKHTRDALRQLEQ
                     NYHDSSS"
     gene            4729..4821
                     /locus_tag="O2701_001356"
     CDS             4729..4821
                     /locus_tag="O2701_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293257.1"
                     /translation="MESIFKIKLMNGICRSENMNMKKKNKGEKI"
     gene            4935..5936
                     /locus_tag="O2701_001357"
     CDS             4935..5936
                     /locus_tag="O2701_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCZ4293258.1"
                     /translation="MVKLSVLDYALIDEGKDAQKALQDSVTLAKLADRLGFKRIWFTE
                     HHNVPAFACSSPELLMMHTLAQTNHIRVGSGGVMLPHYRPYKIAEHFRMMAALYPNRI
                     DLGIGNNPGTTMVKQALDGINPTYDSYDESISLLRDYLTIKDKPSAHTLGVQPHIDHF
                     PEMWLLSSSATSAKIAAELGIGLSVGTFLLPDINAIHAAKDNIDIYKKHFQASTIKMD
                     AKVMASVFVIVADNEAEVAALQHALDVWLLGKLQFAEFEDFPSVDTAQKYKLNDRDKE
                     MIQAHQARIIAGTQEKVKAQLDDFIATFEVDEVLVAPLIPGIEQRCKTLKLLAEIYL"
     gene            5970..6302
                     /locus_tag="O2701_001358"
     CDS             5970..6302
                     /locus_tag="O2701_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HCZ4293259.1"
                     /translation="MKKLANYLWVEKVGDLYVFSMTPELQDDIGTVGYVEFVSPDEVK
                     VDDEIVSIEASKTVIDVQTPLSGTIIERNTKAEEEPTILNSEKPEENWLFKLDDVDKE
                     AFLALPEA"
     gene            6303..7103
                     /locus_tag="O2701_001359"
     CDS             6303..7103
                     /locus_tag="O2701_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HCZ4293260.1"
                     /translation="METLKSNKARLEYLINDMHRERNDNDVLVMPSSFEDLWELYRGL
                     ANVRPALPVSDEYLAVQDAMLSDLNRQHVTDLKDLKPIKGDNIFVWQGDITTLKIDAI
                     VNAANSRFLGCMQANHDCIDNIIHTKAGVQVRLDCAEIIRQQGRNEGVGKAKITRGYN
                     LSAKYIIHTVGPQIRRLPVSKMNQDLLAKCYLSCLKLADQHSLNHVAFCCISTGVFAF
                     PQDEAAEIAVRTVESYLKETNSTLKVVFNVFTDKDLQLYKEAFNRDAE"
     gene            7093..8037
                     /locus_tag="O2701_001360"
     CDS             7093..8037
                     /locus_tag="O2701_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HCZ4293261.1"
                     /translation="MQSSKWNAMSLLMDDKTKQAEVLRTAIDEADAIVIGIGAGMSAS
                     DGFTYVGERFTENFPDFIEKYRFFDMLQASLHPYGSWQEYWAFESRFITLNYLDQPVG
                     QSYLALKSLVEGKQYHIITTNADNAFDVADYDMTHVFHIQGEYILQQCSQHCHAQTYR
                     NDDLIRKMVVAQQDMLIPWEMIPRCPKCDAPMEVNKRKAEVGMVEDAEFHAQLHRYNA
                     FLEQHQDDKVLYLEIGIGYTTPQFVKHPFQRMTRKNENALYMTMNKKAYRIPNSIQER
                     TIHLTEDISTLITAALRNDSTTKNNNIGETEDVLNRTD"
     gene            8015..9037
                     /locus_tag="O2701_001361"
     CDS             8015..9037
                     /locus_tag="O2701_001361"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCZ4293262.1"
                     /translation="MYLIEPIRNGEYITDGAIALAMQVYVNQHIFLDEDILFPYYCDP
                     KVEIGRFQNTAIEVNQDYIDKHSIQVVRRDTGGGAVYVDKGAVNMCCILEQDTSIYGD
                     FQRFYQPAIKALHTLGATDVVQSGRNDLTLNGKKVSGAAMTLMNNRIYGGYSLLLDVN
                     YEAMDKVLKPNRKKIASKGIKSVRARVGHLREALDEKYRDITIEEFKNLMVTQILGID
                     DIKEAKRYELTDADWEAIDELADKKYKNWDWNYGKSPKYEYNRSERLSSGTVDITISV
                     EQNRIADCRIYGDFFGQGDIKDVEEALQGTKMTREDLTHQLKQLDIVYYFSNVTVEAL
                     VDMILS"
     gene            9352..10377
                     /locus_tag="O2701_001362"
     CDS             9352..10377
                     /locus_tag="O2701_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HCZ4293263.1"
                     /translation="MNNKVLATGGTGFVGMRIISRLLEQGYDVQTTIRDLSKADKVIK
                     TMQDNGISTERLMFVEADLSQDEHWDEAMKDCKYVLSVASPVFFGKTDDAEVMAKPAI
                     EGIQRILRAAEHAGVKRVVMTANFGAVGFSNKDKNSITNESHWTNEDEPGLSVYEKSK
                     LLAEKAAWDFVENENTTVEFATINPVAIFGPSLDAHVSGSFHLLENLLNGSMKRVPQI
                     PLNVVDVRDVAELHILAMTNEQANGKRFIATADGQINLLEIAKLIKEKRPEIAQKVST
                     KKLPDFILSLGAKFNHQAKEGKLLLDMNRNVSNERAKTLLGWEPIATQEEAILAAVDS
                     MAKYHLI"
     gene            complement(10470..11816)
                     /locus_tag="O2701_001363"
     CDS             complement(10470..11816)
                     /locus_tag="O2701_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCZ4293264.1"
                     /translation="MQAILNFIVDILSQPAILVALIAFIGLIVQKKPAATITSGTIKT
                     ILGFLILSAGADVVVRSLEPFGKIFQHAFGVQGIVPNNEAIVSLALKDFGTTAALIMV
                     CGMIVNILIARFTNLKYIFLTGHHTFYMAAFLAIILTVSHIKGWLTIVIGALVLGLIM
                     AVLPALLQPTMRKITGNDQVALGHFGSISYFAAGAVGQLFKGKSKSTEEIKFPKGLSF
                     LRESTISISITMALLYFIACLFAGVSYVHESISDGQNFIVFSLIQGVTFAAGVFIILT
                     GVRLILAEIVPAFKGISEKLVPNSKPALDCPIVFPYAQNAVLIGFFVSFITGVIGMFI
                     LFLFGGVVILPGVVAHFFLGATAAVFGNARGGIKGAIAGAALNGILITFLPLLFLPFL
                     GELGGAATTFSDTDFLAVGIVFGNAVKYMGLFGAILFIIIVGATTILLKGRQKEQQ"
     gene            complement(11831..12115)
                     /locus_tag="O2701_001364"
     CDS             complement(11831..12115)
                     /locus_tag="O2701_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCZ4293265.1"
                     /translation="MKILVVCGHGLGSSFMVEMNAQEALRQLNAPSDIEVEHSDIMTA
                     SPEMADLFICGRDLAENAERLGDVLVLDNILDKAELQQKLSEKLQQLNMI"
     gene            complement(12117..12560)
                     /locus_tag="O2701_001365"
     CDS             complement(12117..12560)
                     /locus_tag="O2701_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCZ4293266.1"
                     /translation="MSLDILSTTRIIVKEQVNDWTEAITIASQPLLQEQIIEQGYVQA
                     MIDSVNELGPYIVIAPEIAIAHARPNNDVHQVGLSLLKLNQHVAFCDEDHYASLIFVL
                     SAIDNHSHLSVLQNLATVLGDNQTVQQLLTATNAQDIKNILKEHD"
     gene            complement(12565..14520)
                     /locus_tag="O2701_001366"
     CDS             complement(12565..14520)
                     /locus_tag="O2701_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCZ4293267.1"
                     /translation="MLSYRQIEILYLLIHEQTFIPINTIANQLGVSPRTIQYDIAYIE
                     QYAETYHYQVSRNKAAGIKVTTAHATLLNELEHNLTNQIHFSKDERLTHIALKLFETT
                     DPVSTKQLAQDVNVSRRTIADDIKMIQAQLDQYHLKLNYVHNKGFNIIGEEDHYRKAY
                     AHFIHQYMKQAAPFIEADIFNSESIALVRRAIIKTLNSENYHLVQSAIDGLIYHILIA
                     IQRLNENFSFDIPINEIDKWRHTNQYAIASKMIENLERSCNVTFPESEIIFITLHLLG
                     SKMTEHTASSITFEYHDLSQNIHELITCVSQELGIDMSKDNKLHTSLITHIKPAIHRI
                     KYDMLQPNPLRQEVMRRYPQIIEAVSKHISPIEQDAAIRFNEDELTYITIHFASSIER
                     VATHKQSMIKVVLLCGSGIGTSQLLKSKLNHLYPEFHIWDAYSIYQLEESRLLQDNID
                     YVISTVPCEISAVPVIHVDPFINQQSRQKLNQIINDSREQRVMKMATDGKSLADLLPE
                     HRIIINKQPLSIESAIAVAVQPLINDGIVYSNYTAAILKQFEQFGSYMVISPHIALIH
                     AGTDYVQNGVGFALTYFTEGIIFGSKANDPVHLVITLATDHPNAHLKALGQLSECLSN
                     DLYRQDFLDGNIFKIKQHIALTMTKEA"
     gene            14732..15151
                     /gene="mepR"
                     /locus_tag="O2701_001367"
     CDS             14732..15151
                     /gene="mepR"
                     /locus_tag="O2701_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor MepR"
                     /protein_id="HCZ4293268.1"
                     /translation="MEFTYSYLFRMISHEMKQKADQKLEQFDITNEQGHTLGYLYAHQ
                     QDGLTQNDIAKALQRTGPTVSNLLRNLERKKLIYRYVDAQDTRRKNIGLTTSGIKLVE
                     AFTSIFDEMEQTLVSQLSEEENEQMKANLTKMLSSLQ"
     gene            15258..16613
                     /gene="mepA"
                     /locus_tag="O2701_001368"
     CDS             15258..16613
                     /gene="mepA"
                     /locus_tag="O2701_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498905.1"
                     /note="MepA (multidrug export protein A), as described
                     originally in Staphylococcus aureus, is a MATE transporter
                     shown to be over-expressed after culturing in the presence
                     of tigecycline, owing to mutation of its repressor MepR,
                     and then to be one of several factors contributing to
                     elevated tigecycline resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MepA"
                     /protein_id="HCZ4293269.1"
                     /translation="MKDEQLYYFEKSPVFKAMMHFSLPMMIGTLLSVIYGILNIYFIG
                     FLEDSHMISAISLTLPVFAILMGLGNLFGVGAGTYISRLLGAKDYSKSKFVSSFSIYG
                     GIALGLIVILVTLPFSDQIAAILGARGETLALTSNYLKVMFLSAPFVILFFILEQFAR
                     AIGAPMVSMIGMLASVGLNIILDPILIFGFDLNVVGAALGTAISNVAAALFFIIYFMK
                     NSDVVSVNIKLAKPNKEMLSEIFKIGIPAFLMSILMGFTGLVLNLFLAHYGNFAIASY
                     GISFRLVQFPELIIMGLCEGVVPLIAYNFMANKGRMKDVIKAVIMSIGVIFVVCMSAV
                     FTIGHHMVGLFTTDQAIVEMATFILKVTMASLLLNGIGFLFTGMLQATGQGRGATIMA
                     ILQGAIIIPVLFIMNALFGLTGVIWSLLIAESLCALAAMLIVYLLRDRLTVDTSELIE
                     G"
     gene            16717..17157
                     /locus_tag="O2701_001369"
     CDS             16717..17157
                     /locus_tag="O2701_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HCZ4293270.1"
                     /translation="MCKSKILLKNIFSEESEVKDLTEEKYNQDYEALTFSFKEETYQS
                     RLAKKTPTKSGYFVTCWTKDEDNYNRPYKIEEFADYLIVAVIDDELNGYFLFPRELLV
                     EKGILASSKYQGKMAFRVYPKWCNQLNKTAGQTQKWQCKYFFEY"
     gene            complement(17240..18598)
                     /gene="glpT"
                     /locus_tag="O2701_001370"
     CDS             complement(17240..18598)
                     /gene="glpT"
                     /locus_tag="O2701_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCZ4293271.1"
                     /translation="MNFLKPAKHIKPLPENQIDDTYKRLRLQVFLGIFIGYAGYYLLR
                     KNFSLAMPALQEQGFTKAELGFALSAVSIAYGFSKFFMGTVSDRSNARIFLVLGLALT
                     AIVNLLMGFVPFFTSGIGIMFVLLFLNGWFQGMGWPPSGRVLVHWFSVSERGSKTALW
                     NVAHNVGGGIMAPIAAWGITTTAFINFGYLKGFEGVFIYPALLALIIAAISYVLIRDT
                     PQSQGLPPIEIYKNDFATSDKKTLETELTTKEILFKYVLNNKWVWAIAFANIFVYFVR
                     YGVLDWAPVYLSEEKHFDLKASGWAYFLYEWAGIPGTLLCGYISDKLFKGRRGPAGFF
                     FMLGVTVFVLIYWLNPPGNAWLDNVSLIAIGFLIYGPVMLIGLQALDYVPKKAAGTAA
                     GLTGLFGYLFGAVMANIVLGAVVDKFGWDVGFILLTAISVFAMLSFILTWNKVGQETV
                     HH"
     gene            18906..19832
                     /locus_tag="O2701_001371"
     CDS             18906..19832
                     /locus_tag="O2701_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCZ4293272.1"
                     /translation="MNIVGHHHISMYTKDAKRNKDFYTNVLGLRLVEKSVNQDNPSMY
                     HLFYGDEVGTAGTILSFFEIPNAGHKQPGTETIYRFSLLVPNQAALHYFEKRLENNGI
                     KSERLYYLGQEGVVFKDEDDLEIILLVNDSFEVPHQWQHNAYSEIPQAYQILGIGPVE
                     LRVRNAARTVEFLENVLGYRKRDNKSFDVLTLAPQGLYSDFVVIEQQGQRERPGRGYI
                     HHIAVNTPQMSDLDAIYKKLQQQPQSNSGIIDRYFFKSLYYRHNSIMYEFATEAPGFT
                     IDTPVEQLGSQLNLPDFLEAEREQIESKLHEI"
     gene            19846..20907
                     /locus_tag="O2701_001372"
     CDS             19846..20907
                     /locus_tag="O2701_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCZ4293273.1"
                     /translation="MAKLEMNKNTPLEFGLYSLGDHLLNPLKGEKVSYEQRINEIIEA
                     SKLADEAGIDVFAVGESHQEHFTTQAHTVVLGAIAQATKHIKVSSSSTIISATDPVRV
                     FEDFATLDLISHGRAEIVAGRASRTGIFDLFGYDLKDYDELFEEKLGLLLELNKTERI
                     TWSGKYRPELRNMKIFPRPIDNILPIWRAVGGPPASAIKAGKQGVPMMITTLGGPAMN
                     FKGSIDAYRQAATEAGFDASPKSLPVSTASLFYTAETTQDAMREFYPHLNTGMSFIRG
                     VGYPKQQFANSSDYREALMVGSPQQIIEKILYQHELYGHQRFMAQLDFGGVPFENVMK
                     NIELIGNDIIPAIKKHLSK"
     gene            20921..21487
                     /locus_tag="O2701_001373"
     CDS             20921..21487
                     /locus_tag="O2701_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCZ4293274.1"
                     /translation="MNIVLLSGSTVGSKTRIAMDDLKNELEVINEGHQIELMDLRELE
                     LEFSVGKNYLDTTGDVYKLTTSLMQADVIFIGFPIFQASIPGALKNVFDLLPVNAFRD
                     KVIGLVATAGSSKHYLIPEMHLKPILSYMKAHTMQTYVFIEEKDFSNQQIVNDDVVFR
                     LKALAQSTMRTAKVQQQVFEEENNQYDF"
     gene            complement(21547..22542)
                     /locus_tag="O2701_001374"
     CDS             complement(21547..22542)
                     /locus_tag="O2701_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCZ4293275.1"
                     /translation="MASLKNKHFMIGLSLTFIVALFSFLAAKLPILDKVGALTIAILI
                     AILYRHFRGYPEQYSSGITFSSKYLLRFAIILYGLKLNIFDIIGQGSKLLAIDVGVVI
                     FSIVMMLFVNKLLHGDKNIALLLGVGTGVCGAAAIAAVAPIFKSREKDTAISIGIIAL
                     IGTIFSLIYTAIYAIFSMTTNVYGAWSGVSLHEIAHVVLAGGFGGSDALKIALLGKLG
                     RVFLLIPLTIVLILIMRFRSSESSSKGRISIPYFLIGFVIMALVNTYVTIPSALLNIL
                     NTVSTICLLMAMVALGLNVAFKDLKNRALKPLMTIIITSICLSSLAFIVVHWLYS"
BASE COUNT         7742 a         3565 c         4438 g         7032 t
ORIGIN      
        1 atgttgttgt gttgattcaa caccacaact aaaagtgttt ataaattatt tggaaataca
       61 catatttgta aatgattagt atcgatttaa tatcgtatta ttaaattttt attaattttg
      121 tagtcttaat caaaaaataa tatatgtcat gttatattga aggtgcagtt gtttttcatt
      181 ctcaagaggg ggtcaaaaaa atacttttga ggtgattata tgttaagagg acaagaagaa
      241 agaaagtata gtattagaaa gtattcaata ggcgtggtgt cagtgttagc ggctacaatg
      301 tttgttgtgt catcacatga agcacaagcc tcggaaaaaa catcaactaa tgcagcggca
      361 caaaaagaaa cactaaatca accgggagaa caagggaatg cgataacgtc acatcaaatg
      421 cagtcaggaa agcaattaga cgatatgcat aaagagaatg gtaaaagtgg aacagtgaca
      481 gaaggtaaag atacgcttca atcatcgaag catcaatcaa cacaaaatag taaaacaatc
      541 agaatgcaaa atgataatca agtaaagcaa gattctgaac gacaaggttc taaacagtca
      601 caccaaaata atgcgactaa taatactgaa cgtcaaaatg atcaggttca aaatacccat
      661 catgctgaac gtaatggatc acaatcgaca acgtcacaat cgaatgatgt tgataaatca
      721 caaccatcca ttccggcaca aaaggtaata cccaatcatg ataaagcagc accaacttca
      781 actacacccc cgtctaatga taaaactgca cctaaatcaa caaaagcaca agatgcaacc
      841 acggacaaac atccaaatca acaagataca catcaacctg cgcatcaaat catagatgca
      901 aagcaagatg atactgttcg ccaaagtgaa cagaaaccac aagttggcga tttaagtaaa
      961 catatcgatg gtcaaaattc cccagagaaa ccgacagata aaaatactga taataaacaa
     1021 ctaatcaaag atgcgcttca agcgcctaaa acacgttcga ctacaaatgc agcagcagat
     1081 gctaaaaagg ttcgaccact taaagcgaat caagtacaac cacttaacaa atatccagtt
     1141 gtttttgtac atggattttt aggattagta ggcgataatg cacctgcttt atatccaaat
     1201 tattggggtg gaaataaatt taaagttatc gaagaattga gaaagcaagg ctataatgta
     1261 catcaagcaa gtgtaagtgc atttggtagt aactatgatc gcgctgtaga actttattat
     1321 tacattaaag gtggtcgcgt agattatggc gcagcacatg cagctaaata cggacatgag
     1381 cgctatggta agacttataa aggaatcatg cctaattggg aacctggtaa aaaggtacat
     1441 cttgtagggc atagtatggg tggtcaaaca attcgtttaa tggaagagtt tttaagaaat
     1501 ggtaacaaag aagaaattgc ctatcataaa gcgcatggtg gagaaatatc accattattc
     1561 actggtggtc ataacaatat ggttgcatca atcacaacat tagcaacacc acataatggt
     1621 tcacaagcag ctgataagtt tggaaataca gaagctgtta gaaaaatcat gttcgcttta
     1681 aatcgattta tgggtaacaa gtattcgaat atcgatttag gattaacgca atggggcttt
     1741 aaacaattac caaatgagag ttacattgac tatataaaac gcgttagtaa aagcaaaatt
     1801 tggacatcag acgacaatgc tgcctatgat ttaacgttag atggctctgc aaaattgaac
     1861 aacatgacaa gtatgaatcc taatattacg tatacgactt atacaggtgt atcatctcat
     1921 actggtccat taggttatga aaatcctgat ttaggtacat ttttcttaat ggctacaacg
     1981 agtagaatta ttggtcatga tgcaagagaa gaatggcgta aaaatgatgg tgtcgtacca
     2041 gtgatttcgt cattacatcc gtccaatcaa ccatttgtta atgttacgaa tgatgaacct
     2101 gccacacgca gaggtatctg gcaagttaaa ccaatcatac aaggatggga tcatgtcgat
     2161 tttatcggtg tggacttcct ggatttcaaa cgtaaaggtg cagaacttgc caacttctat
     2221 acaggtatta taaatgactt gttgcgtgtt gaagcgactg aaagtaaagg aacacaattg
     2281 aaagcaagtt aaattcatct tctgaattta atatgctatg taaatcgtgc tgttatcacg
     2341 gcacatcaga tataagtagc atcacagtgt tgaatttaaa aatagtaaag tgaaataaag
     2401 cgcctgtctc attagcgaaa actaaaggga caggcgtatc tgtttatgag cttaataaat
     2461 tgtatgaata atatggttga tcgaataact gtttatcatg atgataaatt gagtttttta
     2521 aaataatgat atattacatc attgttatag cgtttaagaa atcaacaact ttacgataaa
     2581 tagtgattgc ttcgtcatta ggtctacgat caaaatcatg ctcgttttta ttcacgcgtt
     2641 caaatgttga atgtggaaca tgattcatga tatgttcgct ttcctcaacg ggaacatcat
     2701 aatcgccatt acaatgcgca atgaaaacag gtggaagtgt tttaagttca tctggtgcaa
     2761 tattatattt tgaattagta taatcagcaa tgttaatcat atttatccat ttacctgtgc
     2821 cacgtgcata aacgtagatt aaaaaacgtt gtgcgatttg atcttgaaca accggtgttg
     2881 gtgaagtgag ttgtgcaatc attgtttcgt ttacgctttg agctattttt gcgtaataac
     2941 tattagttgt tttaaaaggt tcagtgttga tgcgactata accataaaaa tcaataacac
     3001 catcaatatc tctgtctcgt gcaattaata gacttaaata tgcacctgat gatctgccaa
     3061 aggtaaaaat agggcaatta gaatattgtg attgaatcgc atcgaatgat gcgtagacat
     3121 cctcaataat gcaatcgaga cttacttctg gtaataaacg ataacttagt tgaattaaat
     3181 cgtaatgttc cgtaaggata tcgatatact gtggggataa atcgttagct ttaccgaaca
     3241 ttaatccacc accgtggatg tagacaatag cgccttttgt tggttgattt tttgctttaa
     3301 taattgtgta aggtaatgca aatgcatctt tagtaattac tttatcttta atttcagtca
     3361 cgatttaata ggctccttat ttttgatatt gatgtcatta taacactgtc ttaaatttcc
     3421 atgaaaaata gtcttaagac gatgagtcat gataattctg ttccaattga cgtaaagcgt
     3481 cacgggtatg cttctttaga ccttccccat aatccatcat tttaacaata tctttaaaag
     3541 cagcatgtgg aatggctaaa tcttctaaat ctgccataga aaattcaaga ttgatatcat
     3601 gtggtcgctg ttcagcaagt ttatgcacaa agtcaggttc tgtgacaaaa ggcgaagaca
     3661 tgccgaccat atctgcatgt tgtaaagcat ctaaagcaga ctctggagaa ttaatcccgc
     3721 cacttgcaat taaagggata cgacctgcta aatgttcata gacaatttgg ttaactggtc
     3781 gaccgaaatg atcacctggt gtacgagacg tattttgata aatatgtcga ccccagctag
     3841 cgattgctaa gtattggatg tttgaaacgt ccatgaccca attgattaat tggttgaact
     3901 cgtcaatggt atatcctaaa tcactgcctc tggtttcttc tggcgttgct cgaaatccta
     3961 aaataaaatt gtcaggtgct tctttatcaa tcacttcttg taccgcacgc ataacttcta
     4021 aacataatct tgcacgattt tttaatgagt cggcaccgta atggtctgta cgtttattcg
     4081 aaaaagttga gaaaaatgtt tgaatcagca aacgttgtgc aatcgaaatt tccacaccat
     4141 caaaacctgc tttaatcgcg cgtaatgtag catcgcgata ctgctgaatg atgctattga
     4201 ttttctcatg agacatggcg ataacatcgt gttcaatcgg tgaatgcaat gtcatagggc
     4261 ttggtccata cacctttcca aaatttaaaa tggcttgatt tgaaaaacga ccagcatgcg
     4321 ctagctggat aatagcgagg ctaccatgtt gtttcatcgt agatgccatg ttagttaatc
     4381 cagggataca agcatcatga tcaatattaa agccatattc aaacaattga ccataaggtt
     4441 caatgtaagc agcgccggtg acttgcattc cagctgaatt agagcgacgt gcagcataag
     4501 ccaagtcttc ttttgtaata tagccttctt ttgttgatgt gtttacggtc attggtgata
     4561 atacaaagcg attcgaaatt ttgatgccat taggtaagtg gattgattgt aaaagtggtt
     4621 tgtatcggta catactatga ttccttttct attcaatatt gttttcaaag taccatggaa
     4681 agaatgaata atcaatgatg aacagtcttg atagaataga attggtacat ggaaagtatt
     4741 tttaaaatta aactaatgaa tggcatttgt aggtctgaaa atatgaatat gaaaaagaaa
     4801 aataaaggcg aaaagatata aaagttaatt gaaaaacgtt atcatatacg tgggtatatg
     4861 aagagggaat ggtattaaga acgctaaaat gttatgtcgg tttgacatga caggataagt
     4921 ttggagatga cggattggtt aaattaagcg tattagacta tgccttaata gatgaaggta
     4981 aggatgcaca aaaggcattg caagattcag tgacacttgc aaaattagca gatcgacttg
     5041 gctttaagcg aatttggttt acggaacatc ataatgtacc agcgtttgcg tgtagtagtc
     5101 cagaactttt gatgatgcat acattggcgc agacaaatca catacgagtt ggctctggtg
     5161 gtgtgatgct gccgcactat cgaccttata aaattgctga gcattttaga atgatggcag
     5221 cgttatatcc aaatcgtatt gatttaggta ttggtaataa tccaggtact actatggtaa
     5281 agcaagcttt agatggaata aatcctacat atgatagtta cgatgaatcg atttcgttat
     5341 tacgtgatta tcttacaata aaggataaac caagtgcgca tacgttaggt gtccaaccac
     5401 acattgatca ttttccagaa atgtggttat taagtagtag cgcaacatct gccaaaatag
     5461 ctgccgaact aggtataggg ctttctgttg gaacattttt gctaccagat ataaatgcga
     5521 tacatgcagc gaaggataac attgatattt acaaaaaaca tttccaagca tcaacgatta
     5581 aaatggacgc aaaggtgatg gcatctgtat ttgtcattgt agctgataac gaagcggaag
     5641 tagcagcatt acaacatgcc ttagatgttt ggttattagg taaattacaa tttgcagaat
     5701 ttgaagattt tccttcagta gacacagcac aaaagtataa gcttaatgat cgagacaaag
     5761 agatgattca agcacatcaa gcacgcatca ttgcaggtac acaagaaaag gttaaagcac
     5821 aattagatga tttcattgct acgtttgaag ttgatgaggt gttagtagca ccgcttattc
     5881 caggtattga acagcgttgt aaaacattaa aattactcgc ggaaatttat ttgtagcatt
     5941 ttaaatagaa gagaaaggat gaagataaga tgaaaaagtt agccaattat ttatgggtag
     6001 aaaaagtagg agatttgtat gtgtttagta tgacacctga attgcaagat gatattggga
     6061 cagtaggtta tgttgaattc gtaagtccag atgaagttaa agtggatgat gaaattgtga
     6121 gtatcgaagc atcgaaaacg gtcattgatg tgcaaacgcc attgtcagga acgattattg
     6181 agcgaaatac aaaagcggaa gaagaaccga caattttaaa ctctgaaaaa ccagaagaaa
     6241 attggttgtt caaattggat gatgtcgata aagaagcatt cctagcatta ccggaggctt
     6301 aaatggaaac gttaaaatca aataaagcga gacttgaata tttaatcaat gatatgcatc
     6361 gagagagaaa tgacaatgac gtattggtaa tgccatcttc atttgaagat ttgtgggaat
     6421 tatatcgagg cttagcaaat gtcagaccgg cattacctgt aagtgatgaa tatttagctg
     6481 tacaagatgc tatgttaagt gatttgaatc gtcaacatgt tacggatttg aaggatttga
     6541 agccgataaa aggtgacaat atctttgttt ggcaaggtga tatcacgacg ttaaaaatcg
     6601 atgctattgt taatgctgca aatagtcgtt ttctaggatg tatgcaagct aatcatgact
     6661 gcattgataa tattattcat acaaaagcgg gtgttcaagt tcgacttgat tgtgcagaga
     6721 tcattcgaca acaagggcgc aatgaaggtg taggtaaagc caaaataaca cgtggatata
     6781 atttgtcagc aaagtatata attcatacgg ttggtccgca aatacgtcga ttgcctgttt
     6841 caaagatgaa tcaggacttg ttagctaaat gttatcttag ctgtcttaaa ttggctgatc
     6901 aacatagttt aaatcatgtc gctttttgct gtatatctac aggtgtattt gcttttcctc
     6961 aagatgaagc agcagaaatt gctgttcgaa cagtagaaag ctatctcaaa gaaacaaatt
     7021 caacattgaa agtcgtgttc aatgtattta cagataagga tttacaactg tataaggagg
     7081 catttaaccg tgatgcagag tagtaagtgg aatgcaatgt ctctgttaat ggatgacaag
     7141 acaaagcagg ctgaagtatt gcgtactgcg attgatgaag cagatgcgat agtgattgga
     7201 attggtgcag gcatgtctgc atctgacgga tttacatatg taggagagcg ttttacggaa
     7261 aatttcccag attttattga aaaatatcgc ttctttgata tgttgcaagc gagtttacat
     7321 ccttatggca gttggcaaga gtattgggca tttgagagtc gttttattac attaaactat
     7381 ttagatcaac ctgtaggtca gtcttacctc gctttaaaat ccttggtgga aggtaaacag
     7441 taccacatta taactacgaa tgcagataat gctttcgatg tagctgatta tgatatgact
     7501 catgtatttc atatacaagg ggagtatata ctgcaacagt gtagtcagca ttgtcatgct
     7561 caaacgtatc gcaatgatga tttaattcgt aaaatggttg ttgcgcaaca agatatgctt
     7621 ataccttggg agatgattcc aagatgtcca aaatgtgatg ccccaatgga agtgaataaa
     7681 cgtaaagcgg aagttgggat ggttgaagat gctgaatttc atgcgcaact acatcgttat
     7741 aatgcttttc tagagcaaca tcaagatgat aaagtgttgt atttggaaat tggaattggt
     7801 tatactacac cacaatttgt gaagcatcct tttcagcgta tgacacgtaa aaatgaaaat
     7861 gccctttata tgacgatgaa taaaaaggca tatcgcattc cgaattcaat tcaagaacgt
     7921 accatacatt taactgagga tatctcaaca ttgattacag cagcactccg gaacgacagc
     7981 acaacgaaaa ataacaacat tggagagaca gaagatgtac ttaatagaac cgattagaaa
     8041 tggagaatat attactgatg gtgcgattgc actcgctatg caagtttatg ttaaccagca
     8101 tatcttttta gatgaagata ttttattccc ttattattgt gatccaaaag tggaaattgg
     8161 acgttttcaa aatactgcta tagaagtgaa tcaagattat atagataaac acagtattca
     8221 agtagttcgc cgagatactg gtggtggcgc tgtgtatgtt gataaaggtg ccgttaatat
     8281 gtgttgtatt ttagaacaag acacttcaat ttatggtgat tttcaacgat tttatcaacc
     8341 agctataaag gcgttgcata cattaggtgc aacagatgtg gtacaaagcg gtagaaatga
     8401 tttaacattg aatggtaaaa aagtgtcagg cgccgcaatg acattaatga ataatcgtat
     8461 ttatggcggt tattcgctat tacttgatgt taattatgaa gcaatggata aagtgttaaa
     8521 gcctaatcgc aaaaagattg catcgaaagg gattaaatct gtgcgcgcac gtgttggtca
     8581 tcttagagaa gcactggatg aaaagtatcg tgatataacc attgaagaat ttaaaaattt
     8641 aatggtgacg cagattttgg gaatcgatga cattaaagag gcgaaacgat atgaattaac
     8701 ggatgcagat tgggaagcga ttgatgaatt agctgataaa aagtataaaa attgggattg
     8761 gaattatggc aagtcaccca aatatgaata caatcgaagt gaaagattat cttcaggtac
     8821 ggtagacata acaatttctg ttgaacaaaa tcgtatcgca gattgtcgta tttatgggga
     8881 tttctttgga caaggtgata taaaagatgt ggaagaagca ttacaaggaa caaaaatgac
     8941 aagagaagat ttaacgcatc agttaaagca attagacatc gtttattatt ttagcaatgt
     9001 tacggtagaa gcattagtgg atatgatttt aagttaatat tgttatttta tgtatgctga
     9061 atcattggaa gtgtttgctt gctcttgaaa aggtgacaat agtgtttggt gaaggttgaa
     9121 catatgagtg gaaattattg cctttaacta ttcaaagtat gatatatata tggtttttgt
     9181 ttctaaatga ttgggtattt gaaaatagat gagtttaata ttttaaggaa tataatgatg
     9241 tttactttta taattcatat agaatattaa gcaatataag tctgttgata tatacaaaat
     9301 ataatgactg ctataatgag taatcaatag acacaaagag gagattatgt gatgaataat
     9361 aaagtattag caaccggtgg tacagggttt gttggcatgc gaattatttc acgattatta
     9421 gaacaaggtt atgacgtaca aacgacgata cgtgatttaa gtaaagctga taaagtaatt
     9481 aaaacaatgc aagacaatgg catttccaca gagcgattaa tgtttgtcga agcggattta
     9541 tcacaagatg aacattggga tgaagcaatg aaagattgca agtatgtctt gagtgtagca
     9601 tctccggtgt ttttcggtaa aacagacgat gcagaagtga tggcgaagcc tgcaattgaa
     9661 ggtatacaac gtattttaag agctgcagaa catgcgggtg ttaaacgtgt ggtaatgact
     9721 gcaaactttg gtgcagttgg ttttagtaat aaagataaaa attcaatcac aaatgaaagt
     9781 cattggacaa atgaagatga accaggctta tcagtatatg aaaaatcaaa attgttagct
     9841 gaaaaggcag cgtgggattt tgttgagaat gaaaatacaa cagtagaatt tgccacaatc
     9901 aatccagttg caatttttgg gccatcatta gatgcacacg tttcaggaag ctttcattta
     9961 ttagaaaatt tattgaatgg ttcaatgaaa cgtgtaccgc aaattccgtt aaatgttgtt
    10021 gatgtgagag acgtagctga actgcacatt ttggcaatga caaatgaaca agctaatggc
    10081 aagcgattta ttgcgacggc tgatggacaa attaatttgt tggaaattgc aaaattaatt
    10141 aaagaaaaga gacctgaaat agctcaaaaa gtttctacta aaaaattacc agactttatt
    10201 ttgagtctag gtgctaaatt taatcatcaa gctaaagaag gtaaactttt attagatatg
    10261 aaccgcaatg taagtaacga acgtgcaaaa acattacttg gttgggaacc gattgcgaca
    10321 caagaagaag caattttagc agctgtcgat agtatggcta agtatcattt aatataatac
    10381 aaaacaccgt ccataagtaa gtggctttcc tcttgataaa aggggcattc actcaatatg
    10441 tgacggtgtt ttatatttct acgttaacac tattgctgtt ctttttgacg gccttttaat
    10501 aaaattgttg tcgcacctac gataataata aatagaatcg caccaaataa tcccatatat
    10561 tttactgcgt taccgaacac gataccgaca gctaaaaagt ctgtatctga gaatgttgtt
    10621 gcagcaccac ctaattcgcc taaaaatggc aagaataata atggtaaaaa cgtgattagg
    10681 ataccattta gagcggcgcc agcaatagca cctttaatac cgcctcttgc attaccgaat
    10741 acagcagccg ttgcacctaa gaagaagtgt gcaactacgc caggtaaaat gacgacgcca
    10801 ccaaataaga ataagataaa cataccgatg acacctgtaa taaagctgac aaagaatcca
    10861 attaatactg cattttgtgc ataagggaac acaatagggc agtctaatgc aggtttagaa
    10921 tttggtacaa gcttttcaga aattccttta aatgctggga cgatttcagc taagattaaa
    10981 cgaacgcccg ttaaaataat aaatacacca gcagcaaatg tcacaccttg aattaatgaa
    11041 aagacaataa agttttgacc atcactaata gattcgtgta cataactaac gcccgcaaat
    11101 aagcatgcga tgaagtaaag taatgccatc gtaatcgaga tactaattgt actttctcgt
    11161 aagaaactta agccttttgg aaatttaatc tcttccgttg atttagactt acctttgaat
    11221 aattgaccta cagcacctgc ggcaaagtaa ctgattgagc caaaatgacc taaagctact
    11281 tggtcattcc ctgtaatttt tcgcatcgta ggttggagta atgcaggtaa tactgccatg
    11341 attaatccta atacgagtgc gccgataaca atcgttagcc agcctttaat atgactgact
    11401 gttaaaatga ttgctaaaaa cgcagccatg taaaatgtat gatgacctgt taaaaagata
    11461 tattttaaat tagtgaagcg ggcaattaaa atattaacaa tcatgccaca gaccatgatg
    11521 agtgcagctg ttgttccaaa atcttttaag gctagtgaga cgatagcttc gttgttaggt
    11581 acgatacctt gcacaccaaa tgcgtgttgg aatattttgc cgaatggttc aagagatcga
    11641 acgacgacat cagcacctgc acttaaaatt aagaagccta atatcgtttt aatggttcct
    11701 gaagtgatcg ttgcggcagg ttttttctga acgattaaac ctataaaggc aatcagtgca
    11761 acaagaatgg ctggttgact taaaatatcg actataaaat taaggattgc ttgcataggt
    11821 cgtacctcct ttaaatcatg ttaagttgtt gtaatttttc tgagagcttt tgttgtaatt
    11881 cagctttatc taaaatatta tcaagaacta agacatcccc tagacgttcg gcattttcag
    11941 ctaaatctct accacaaata aacaagtcag ccatctctgg acttgctgtc ataatgtcac
    12001 tatgttcaac ttcgatatca gatggtgcat taagttgcct aagtgcttct tgtgcgttca
    12061 tttctaccat aaaactactt cctaaaccgt ggccacatac tactaaaatt ttcatattaa
    12121 tcatgctcct ttaaaatgtt tttaatgtct tgtgcatttg ttgcagttaa tagttgctgg
    12181 actgtttggt tatcgcccag tacggttgct aaattttgta atacagataa gtgtgaatga
    12241 ttgtcgatgg cactcaatac aaaaatgaga gatgcgtagt gatcttcatc acaaaatgcc
    12301 acatgttgat tcaactttaa tagacttaaa ccaacttgat gtacgtcatt gttcggtctt
    12361 gcatgtgcaa ttgcaatttc aggtgcgata acgatataag gtccaagttc attaacgcta
    12421 tcaatcattg cttgaacata gccttgttca ataatttgtt cttgtagtaa tggctgagaa
    12481 gctatagtta tagcttcagt ccaatcattt acttgttctt ttacaatgat gcgtgttgtt
    12541 gacaaaatgt ctaatgacac gttattaagc ctcctttgtc atagttaaag caatgtgttg
    12601 tttaatttta aaaatattcc catctaagaa atcttgtcga tataagtcgt tgcttaagca
    12661 ttcgcttaac tgtcccaatg cctttaaatg tgcattgggg tggtccgttg ctaatgtaat
    12721 tacaaggtga acgggatcgt tagctttact accaaagata atcccttcag tgaaatatgt
    12781 tagtgcgaaa cctacaccat tctgtacata atcagtacca gcgtgaataa gtgcaatatg
    12841 tggactaatg accatatatg acccgaattg ttcaaattgt tttaaaattg cagctgtata
    12901 atttgaatag acaatgccat cattgattaa aggttgcaca gccactgcaa ttgcggattc
    12961 aattgataat ggttgtttat ttataatgat gcgatgttca ggcaataaat ctgcgagtga
    13021 cttgccatca gttgccattt tcatgactcg ttgttctctt gagtcattga taatttgatt
    13081 caatttttga cgagattgtt gattgataaa tggatcgaca tgaataactg gtacagctga
    13141 tatttcacaa ggtactgttg aaatgacata atcaatgtta tcttgcaata atcgactttc
    13201 ttccaattga taaatggaat aggcatccca aatgtgaaac tcaggataca ggtgatttag
    13261 ttttgatttt aaaagttgtg acgtgcctat accagaacca catagtaaga caaccttaat
    13321 cattgattgt ttatgtgttg caacacgctc tatacttgat gcgaagtgaa ttgtaatgta
    13381 tgttaattca tcttcgttga agcgaatagc agcatcttgt tcaattggac taatatgctt
    13441 gctaacggct tcaatgattt gaggatagcg acgcataact tcttgcctca aaggattagg
    13501 ttgtagcata tcgtatttaa tacgatgtat agctggtttg atatgtgtga tcagactggt
    13561 atgtaacttg ttgtcttttg acatatcaat gcctaattct tggctaacac aagtgatcaa
    13621 ttcatgtata ttttgcgata aatcatggta ttcaaaggta attgaagatg ctgtatgttc
    13681 agtcatttta gagcctagta aatgtaacgt gataaagata atttcagact ctggaaatgt
    13741 gacattacaa ctgcgttcta agttttctat catttttgaa gcaatagcat actgattagt
    13801 atgtcgccat ttatcaattt cattgatagg tatatcgaac gaaaaatttt catttaaacg
    13861 ctgaatggca atgagtatat gatagattaa gccatcgata gccgactgaa ctaaatgata
    13921 attttcacta tttaatgtct taataatggc acggcgaacc aatgcgattg attctgaatt
    13981 aaagatatcc gcctctataa aaggtgcagc ttgtttcata tattgatgta taaagtgtgc
    14041 atacgcttta cgataatgat cttcctcacc aataatattg aatcctttat tgtggacata
    14101 atttaacttt aaatggtatt gatctagttg ggcttgaatc attttaatat catctgcaat
    14161 tgtccgacgc gaaacattaa catcttgcgc aagttgcttt gttgaaacag gatcggttgt
    14221 ttcgaataac tttaaagcga tatgtgtgag tcgttcatct tttgaaaaat gaatttgatt
    14281 tgttagattg tgctctaatt catttaatag tgtcgcgtga gctgttgtta ctttgatgcc
    14341 tgcagcttta ttacggctga cttggtaatg ataagtttca gcatattgct caatatatgc
    14401 tatatcatat tgaatggtac gaggtgatac accaagttga ttagcaatgg tattgattgg
    14461 aataaacgtt tgctcatgaa ttaaaagata caaaatttcg atttgtctat aacttaacaa
    14521 cgtaatatcc tcctatttgt aattgtaagc gatttcttaa aaacgtagat atgcaatctc
    14581 tttcatattt taatccgaaa aattgcatat caaaatgttt atggcgcaag attttatagg
    14641 aacttttaaa ataaattaga tattcatgtt gtcaatttaa aaatgtcgca gtatatttag
    14701 ttagacatct aacgaaatgg tggtgcaata aatggaattc acttattcgt atttatttag
    14761 aatgattagt catgagatga aacaaaaggc tgatcaaaag ttagagcaat ttgatattac
    14821 aaatgagcaa ggtcatacgt taggttatct ttatgcacat caacaagatg gactgacaca
    14881 aaatgatatt gctaaagcat tacaacgaac aggtccaact gtcagtaatt tattaaggaa
    14941 ccttgaacgt aaaaagctga tctatcgcta tgtcgatgca caagatacga gaagaaagaa
    15001 tatagggctg actacctctg ggattaaact cgtagaagca ttcacttcga tatttgatga
    15061 aatggaacaa acactcgtat cgcagttatc tgaagaagaa aatgaacaaa tgaaagcaaa
    15121 cttaactaaa atgttatcta gtttacaata aatgataagt gtgactggta gaaatcagtc
    15181 actttgtctt taatattata gttagatatc taattgttag taagctaatt attggaaaag
    15241 acaaggagta ttgaacaatg aaagacgaac aattatatta ttttgagaaa tcgccagtat
    15301 ttaaagcgat gatgcatttc tcattgccaa tgatgatagg gactttatta agcgttattt
    15361 atggcatatt aaatatttac tttataggat ttttagaaga tagccacatg atttctgcta
    15421 tctctctaac actgccagta tttgctatct taatggggtt aggtaattta tttggcgttg
    15481 gtgcaggaac ttatatttca cgtttattag gtgcgaaaga ctatagtaag agtaaatttg
    15541 taagtagttt ctctatttat ggtggtattg cactaggact tatcgtgatt ttagttactt
    15601 taccattcag tgatcaaatc gcagcaattt taggggcgag aggtgaaacg ttagctttaa
    15661 caagtaatta tttgaaagta atgtttttaa gtgcaccttt tgtaattttg ttcttcatat
    15721 tagaacaatt tgcacgtgca attggggcac caatggtttc tatgattggt atgttagcta
    15781 gtgtaggctt aaatattatt ttagatccaa ttttaatttt tggttttgat ttaaacgttg
    15841 ttggtgcagc tttgggtact gcaatcagta atgttgctgc tgctctgttc tttatcattt
    15901 attttatgaa aaatagtgac gttgtgtcag ttaatattaa acttgcgaaa cctaataaag
    15961 aaatgctttc tgaaatcttt aaaatcggta ttcctgcatt tttaatgagt atcttaatgg
    16021 gattcacagg attagtttta aatttatttt tagcacatta tggaaacttc gcgattgcaa
    16081 gttatggtat ctcatttaga cttgtgcaat ttccagaact tattatcatg ggattatgtg
    16141 aaggtgttgt accactaatt gcatataact ttatggcaaa taaaggccgt atgaaagacg
    16201 ttatcaaagc agttatcatg tctatcggcg ttatctttgt tgtatgtatg agtgctgtat
    16261 ttacaattgg acatcatatg gtcggactat ttactactga tcaagccatt gttgagatgg
    16321 cgacatttat tttgaaagta acaatggcat cattattatt aaatggtata ggtttcttgt
    16381 ttactggtat gcttcaagcg actgggcaag gtcgtggtgc tacaattatg gccattttac
    16441 aaggtgcaat tatcattcca gtattattta ttatgaatgc tttgtttgga ctaacaggtg
    16501 tcatttggtc attattaatt gctgagtcac tttgtgcttt agcagcaatg ttaatcgtct
    16561 atttattacg tgatcgtttg acagttgata catctgaatt aatagaaggt taaatatttc
    16621 gtccacttct ggctgagtat atttcggtcg gaagtgtatt tttcgaaaaa aataaatata
    16681 tgatacgatt atgaaaaaat aaagtgagat tggcatatgt gtaaatctaa aatactgttg
    16741 aaaaatattt ttagtgaaga atcagaagtt aaagatttaa ctgaagaaaa atataatcaa
    16801 gattacgaag cattaacatt tagctttaaa gaggaaacat atcaaagtag gttagctaag
    16861 aaaacaccga ctaaatcggg atatttcgtg acatgttgga caaaagacga agataattat
    16921 aatcggccat acaaaattga agagtttgct gattacctga ttgttgctgt tatcgatgat
    16981 gaattaaatg gctactttct atttcctagg gaattattgg tagaaaaagg tatcttagct
    17041 tcatctaagt atcaagggaa aatggctttt agagtttatc ctaagtggtg taatcaattg
    17101 aataaaacag cagggcaaac acaaaagtgg caatgtaaat atttttttga atactaataa
    17161 aaagtcatat ggttgaactt ataagagatg aatatatcat ctttcgataa gataaccata
    17221 tgactttatt ttttatcatt taatgatgaa cggtttcttg tcctacttta ttccaagtga
    17281 ggataaagct caacattgca aacacactaa ttgctgttaa taaaataaaa ccgacatccc
    17341 atccgaattt atcaactaca gcacctaaga cgatgttggc cattacagca ccaaacagat
    17401 aaccaaataa tcctgttaat ccagctgctg tgccagctgc ttttttaggt acataatcta
    17461 atgcttgtaa accaattaac ataactggtc catatattaa gaaaccaatg gcaattaatg
    17521 agacattgtc taaccaagca ttgcctggag gatttaacca ataaattaat acaaatactg
    17581 tgacacctaa cataaagaag aaacctgcag gtccacgacg acctttgaat aatttatcag
    17641 aaatgtaacc acataataat gtaccaggaa ttccagccca ttcgtataag aagtatgccc
    17701 aacctgatgc ttttaagtcg aaatgttttt cttcacttaa gtagactggc gcccaatcaa
    17761 gtacaccata acgcacgaaa taaacaaata tatttgcaaa ggcaattgcc catacccatt
    17821 tattgttcag tacatattta aataaaattt cttttgtagt taattctgtt tctaatgttt
    17881 tcttatcgct tgtagcaaag tcatttttat aaatttcgat tggaggtaaa ccttgagatt
    17941 gaggtgtgtc tctaatcaat acgtatgaaa ttgcggcaat gataagtgct aagagtgcag
    18001 ggtaaatgaa tacaccttcg aaacctttta aataaccaaa gttgataaat gcagttgttg
    18061 taatacccca agcagcaata ggtgccataa tacctccacc aacattatgc gcaacgttcc
    18121 aaagggcagt cttacttccg cgttcactta cactaaacca gtgaacgaga acacggcctg
    18181 aaggtggcca gcccatacct tgaaaccatc catttaagaa taataggaca aacataatac
    18241 cgatacctga tgtgaagaac ggtacaaatc ccattaacaa attgacgata gcagtgagtg
    18301 ctaatccaag aactaagaat atccgagcat tgctccgatc acttacagta cccataaaga
    18361 acttactaaa tccatatgcg atggaaacag cagaaagtgc aaaacctagt tccgcttttg
    18421 taaaaccttg ctcttgcaat gccggcatcg ctaacgaaaa gtttttacgt aataaatagt
    18481 acccagcata accgatgaaa ataccaagaa atacttggag acgtaatcgt ttataggtat
    18541 catctatctg attttctggc aaaggcttaa tatgctttgc aggtttaaga aaattcataa
    18601 aatcctcctt aatatgtatt tatatgcatt ttgtgcgata aacttatatt agacatgtat
    18661 gaaagcgttg tcaatatgct tttgtgaaat cttgcatatt tagttattga agtattttat
    18721 aaacatttag aaaaaataaa ttgctacaca taaatttata attatgcaat tattatgtcg
    18781 ttatacatac tagctgtgta tctacgaatg agtacaaaca tatttttatt tgcaaggggt
    18841 aaatggcata taactatctt ttttatgtaa gctggtataa aatttatact aataggaggg
    18901 atagtatgaa tatagtaggg catcatcaca tatccatgta tacaaaagat gcaaaacgta
    18961 ataaggattt ttacacaaat gtccttggat tacgattagt tgaaaagtcg gttaatcaag
    19021 acaatccttc aatgtatcat ttgttttatg gggacgaagt aggtacagcc ggaacaattt
    19081 taagcttttt tgaaattccc aatgcgggtc ataagcagcc aggtactgaa acgatttatc
    19141 gattttcatt attagtacca aatcaagcgg cacttcatta ttttgaaaaa cgtcttgaga
    19201 ataatggtat taagtctgaa cgtttgtact atcttggaca agaaggtgtt gtctttaaag
    19261 atgaagacga cttagaaatc atattgcttg ttaatgatag ttttgaagta ccacatcaat
    19321 ggcaacataa cgcttatagt gaaatacctc aagcatatca aattttagga atagggccag
    19381 tcgaattaag agttagaaat gcagcgcgta cggtagaatt tttggaaaat gtcttaggtt
    19441 atcgcaaaag agataataaa tcattcgatg tgctgacatt agcaccacaa ggtttatatt
    19501 cggattttgt agttattgag caacagggac aacgtgaaag acctggacga ggttatatcc
    19561 atcatattgc agttaataca ccacaaatga gtgacttaga tgcaatttac aagaaattac
    19621 aacaacaacc acaaagtaat tcaggtataa ttgatcgcta tttctttaaa tcattatact
    19681 atcgccataa ttcaattatg tatgaatttg cgactgaagc gcctggattt actattgata
    19741 cacctgttga acaattagga agtcaattga acttgcctga ctttttagaa gcagaacgtg
    19801 aacaaattga aagtaagtta cacgaaatat aaaggagaat gtttaatggc caaattagaa
    19861 atgaataaaa atacgcctct tgagtttggt ttgtattcct taggtgatca tttattgaat
    19921 ccattgaaag gtgaaaaagt tagttatgag caacgtatta atgaaattat tgaagcaagt
    19981 aaattagcag atgaagcagg tattgatgtt tttgcagttg gtgaaagtca tcaggagcat
    20041 tttacaacac aggcacatac ggttgtgtta ggtgcaattg cccaagcgac aaagcatatt
    20101 aaagtttcaa gttcttcaac gattattagt gcaacagatc ctgtaagagt atttgaagac
    20161 ttcgcgacat tagatttgat ttctcatggt agagccgaaa ttgtagctgg cagagcatca
    20221 agaacaggta tttttgactt gtttggctat gatttaaaag actatgatga attgtttgaa
    20281 gaaaaattag gtttactttt agagttaaat aaaactgagc gtattacttg gtctggaaaa
    20341 tatcgtccag aacttagaaa tatgaaaata ttcccaagac caatcgataa tatattgcca
    20401 atatggcgtg ctgttggtgg tccacctgca agtgctatta aagcgggaaa acaaggtgtg
    20461 ccaatgatga ttacaaccct tggtggccca gcaatgaact ttaaaggttc tatagatgct
    20521 tatcgtcaag cggcaactga agcaggtttc gatgcttcgc ctaagtcttt accagtaagt
    20581 acagcgagtc tgttttatac agctgaaaca actcaggatg ctatgagaga attttatcca
    20641 catttgaata cagggatgtc atttattcgt ggtgttggtt atccgaaaca gcaatttgct
    20701 aattcgtcag attatcgaga agcgctaatg gttggaagcc cgcaacaaat tattgaaaag
    20761 atattgtatc aacacgagtt gtatggtcat caacgtttta tggcacagct tgattttggc
    20821 ggtgtgccat ttgaaaatgt tatgaagaat attgagttaa ttggcaacga cattataccg
    20881 gcgattaaaa agcatttatc aaaataggag gggcgtcatc atgaatattg tattattgtc
    20941 aggttccaca gtaggttcta aaacgagaat tgctatggat gatttaaaaa atgaactaga
    21001 agtcatcaat gagggacatc aaatagagtt gatggattta cgagaacttg aattagaatt
    21061 tagcgttgga aagaattatc tagatactac aggagatgta tataaattaa cgacgtcgtt
    21121 aatgcaggct gatgtgattt ttattggttt tccaattttt caagcttcca tccctggtgc
    21181 tttgaaaaat gtgtttgatc tacttccagt caatgcgttt cgtgacaagg taataggact
    21241 tgtagcgaca gcaggttcta gtaaacatta tttaattcct gaaatgcatt taaaaccaat
    21301 attgagttac atgaaagcac atacgatgca aacgtatgta tttattgaag agaaagattt
    21361 ttcaaatcaa caaattgtca atgatgatgt tgtatttcgg ttaaaagcgt tggcacaatc
    21421 cacaatgcga actgccaaag tacaacaaca agtgtttgaa gaagaaaaca accaatacga
    21481 cttttaaagt ataaaaataa gacgctcggc acactaaatt tgtaagtgtt tgagcgtctt
    21541 ttcatattaa ctatatagcc aatgaacgac gataaaggca agtgatgaca agcatattga
    21601 ggtaataatg attgtcataa gcggtttaag tgcgcgattt ttaagatctt taaatgcaac
    21661 atttaaccct aaagcaacca tggccattaa taagcaaatt gttgatacag tatttaaaat
    21721 atttagcaat gctgacggaa tagttacata tgtattcact aaggccataa tgacaaatcc
    21781 aattaaaaag tatggaatgc ttattcgacc cttgctagat gattctgatg aacggaaacg
    21841 cataattaaa ataagtacga tggttaatgg aatcagtaag aatactctac caagtttacc
    21901 aagaagtgca attttaagtg catcactacc accaaagcca ccagctaaga caacgtgtgc
    21961 aatttcatga agactaacac cagaccaagc gccataaaca tttgtcgtca ttgaaaagat
    22021 agcgtagata gctgtatata taagtgaaaa tatcgtacca atcaatgcga tgataccgat
    22081 actaatagct gtatcctttt cacgtgattt gaatattgga gcgactgcgg caatagcagc
    22141 agcaccacaa acgcctgtgc cgacacctag taataatgcg atgtttttgt caccatgcaa
    22201 cagtttgttg acaaagagca tcattacaat actgaaaata acgacaccta catcgatggc
    22261 taatagttta ctaccttgac cgataatatc gaatatattg agtttaagtc catataggat
    22321 gattgcaaat cttaataaat atttagatga aaacgtaata cctgagctat attgttcagg
    22381 atatcctcta aagtgacgat atagaatagc gattaatatc gcgatagtta atgcgccaac
    22441 cttatctagg attggcaatt tagctgctaa aaagctaaat aatgcgacta taaatgttag
    22501 tgatagccca atcataaaat gcttattttt caatgatgcc atgagcagtg cctcctttaa
    22561 tagcatttta gcactgtttt gtcgtatttt taaatataaa tttggaatga ataataaagt
    22621 agtgattaaa ttaagttgtg tgataggaaa cttggacatc aatcaaagta ataggcacta
    22681 caacgcttat tggcggggcc ccaacaaaga agctgacgaa aagtcagctt acaataatgt
    22741 gcaagttggg gatgggcccc aacatagaga aattggg
//
LOCUS       DALAGD010000045        22721 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.045, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000045 DALAGD010000000
VERSION     DALAGD010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22721
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.045"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            132..926
                     /locus_tag="O2701_001375"
     CDS             132..926
                     /locus_tag="O2701_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCZ4293276.1"
                     /translation="MIHSKKLTLGICLVLLIILIVGYVIMTKTNGRNAQIKDTFNQTL
                     KLYPTKNLDDFYDKEGFRDQEFKKGDKGTWIVNSEMVIEPKGKDMETRGMVLYINRNT
                     RTTKGYYFISEMTDDSNGRPKDDEKRYPVKMEHNKIIPTKPLPNDKLKKEIENFKFFV
                     QYGNFKDINDYKDGDISYNPNVPSYSAKYQLNNDDYNVQQLRKRYDIPTKQAPKLLLK
                     GDGDLKGSSVGSRSLEFTFVENKEENIYFTDSVQYTPSEDTRYESN"
     gene            complement(930..1052)
                     /locus_tag="O2701_001376"
     CDS             complement(930..1052)
                     /locus_tag="O2701_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293277.1"
                     /translation="MSYIIKFTFKFFEMATYISQLTLDIELVSSELLVILPGFI"
     gene            1487..1678
                     /locus_tag="O2701_001377"
     CDS             1487..1678
                     /locus_tag="O2701_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293278.1"
                     /translation="MRKEIEALIFSDVSSYDIYVNTGVNQGLIGDIKDGYLTIDSMPY
                     IDAERLYHFAMERKSLVTN"
     gene            complement(1817..2002)
                     /locus_tag="O2701_001378"
     CDS             complement(1817..2002)
                     /locus_tag="O2701_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293279.1"
                     /translation="MLYILITALNVILNKLKMKDSNIISFIYSRHDRILYVNLVGNRF
                     KNNISMTKATSNIGRGF"
     gene            complement(1938..2219)
                     /locus_tag="O2701_001379"
     CDS             complement(1938..2219)
                     /locus_tag="O2701_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCZ4293280.1"
                     /translation="MTYHERVLALRAESKRTAFDFRFEDLFSKEEWLSMSLAERQKAE
                     KAFRHEVKNMDDVRMPFSSVHDAQVKLYNVVYSYNGIKRNFKQVENEGF"
     gene            2508..3203
                     /locus_tag="O2701_001380"
     CDS             2508..3203
                     /locus_tag="O2701_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCZ4293281.1"
                     /translation="MTVLTDKVAVVTGAGSGIGEAIATLLHEEGAKVVLAGRNKEKLQ
                     NVANQLSQDSVKVVPTDVTNKEEVDELIKIAQQTFGGLDIVINSAGQMLSSKITDYQV
                     DEWDSMIDVNIKGTLYTAQAALPTMLEQSSGHLINIASISGFEVTKSSTIYSATKAAV
                     HTITQGLEKELAKTGVKVTSISPGMVDTAITAAYNPTDRKKLEPQDIAEAVLYALTQP
                     KHVNVNEITVRPV"
     gene            3454..3876
                     /locus_tag="O2701_001381"
     CDS             3454..3876
                     /locus_tag="O2701_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCZ4293282.1"
                     /translation="MNKKHVFIIIGVILCICIVASVIYLKVKYDEKEKQKAIYYKEQQ
                     ERITLYLKHNTKEPNTIKTVHFTSLKRGPMGDAVIEGYINENKEDDFVAYGSPEHNYQ
                     FGGSLIKSKNLSTLLKPVHQTKSPDEIKKELESKKNDR"
     gene            4143..4562
                     /locus_tag="O2701_001382"
     CDS             4143..4562
                     /locus_tag="O2701_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCZ4293283.1"
                     /translation="MSKKKILILTSIMLIILISVAGIYLKVKYDEKEKQKAIYYKEQQ
                     ARITLYLKHNTKEPNTIKSVHFTNSETSPMGSAVIEGYINENKKDNFTAYATPEHNYQ
                     FGGAMIESEKLSELLKPANQLKSPDDIKKELNKKKSH"
     gene            4580..4999
                     /locus_tag="O2701_001383"
     CDS             4580..4999
                     /locus_tag="O2701_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCZ4293284.1"
                     /translation="MSKKHVFIIIGVILCICTVSTVMHFKMKYDEKEKQKAIYYKEQQ
                     ERITLYLKHNTKETNTIKSVHFTNLETSPMGSAVIEGYINENKEDDFTAYASPEHNYQ
                     FGGAMIKSEGVDKLLKPAHERKSPEKIKEELDKKEGH"
     gene            5451..5873
                     /locus_tag="O2701_001384"
     CDS             5451..5873
                     /locus_tag="O2701_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCZ4293285.1"
                     /translation="MPYNYKKQNGELMSVMSQGEKFIHQSPVNDELSALIKLLISKIN
                     GCHYCVDIHKKELKELGVTQMKIDEVLSFRHLDLFTDQEKVTLEFAEMLNSIKDFKKF
                     EIIDRLKSFYDEEQIIDLVFVVNQINGWNRLNIISDRL"
     gene            complement(5985..7523)
                     /locus_tag="O2701_001385"
     CDS             complement(5985..7523)
                     /locus_tag="O2701_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HCZ4293286.1"
                     /translation="MTSKHQQKGSIVNRFLNSVEKIGNKLPDPSVLFFLMCVGLAIMT
                     WVISLFNVSVKHPGTHQTIYIKNIISHDGFTMIMNDTIKNFSEFPALGLVLAVMIGIG
                     VAEKTGYFDKLMISVVNRAPRFLILPTIILIGILGSTAGDAATIILPPLAAMLFIKIG
                     YHPIAGLTMAYASAVGGFAANIVVGMQDALVYSFTEPATRIVSDSIKTNVAMNWYFIA
                     ASVVVLLPTILLVTTKLIIPRLGKYDDSLMHDDHEETSSHITDKEAHALKWANISFIV
                     TIILLIITAIPEHSFLRNAKTGSLLDDAPLINGVGLIILVVFLVPGLVYGILSKEIKN
                     TKDLGKMFGDAVGSMGTFIVIVFFAAQLLAYLKWSNLGIIAAVKGAKLLEHQNGIVLI
                     LGIIVLSAMVNMLIGSASAKWGILGPIFVPMLILIGFHPAFTQVIYRVGDSITNPITP
                     MMPYLPLLLTYAQKYDKRMKLGALLSSLMPYSIALSIVWTVFVIIWFLLGIPVGPGGP
                     IFVK"
     gene            7756..8574
                     /locus_tag="O2701_001386"
                     /pseudo
     CDS             7756..8574
                     /locus_tag="O2701_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002503368.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            8837..9202
                     /locus_tag="O2701_001387"
     CDS             8837..9202
                     /locus_tag="O2701_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293287.1"
                     /translation="MLKEKESFRLLYQAIREIADKIGDNQLETNSVSLLLLDFDFEHE
                     VFDELYLVILKYLNTVSIENISHSELLNLIENTIPEDREINTFVKNKIIIGFANNYFP
                     ELQVLANEIKSDMASSLKQ"
     gene            10069..10890
                     /gene="cntK"
                     /locus_tag="O2701_001388"
     CDS             10069..10890
                     /gene="cntK"
                     /locus_tag="O2701_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine racemase CntK"
                     /protein_id="HCZ4293288.1"
                     /translation="MNRQVIEFSKYNPSGNMTILVHSKHDASEYASIANQLMAATHVC
                     CEQVGFIESTQNDDGNDFHLVMSGNEFCGNATMSYIHHLQESHLLKDQQFKVKVSGCS
                     DLVQCAIHDCQYYEVQMPQAHRVVPTTINMGNHSWKAIEIIYETYVHYVIPVKQVTTE
                     IQHLVEAFVREQQWSHKYKTVGMMLFDEQRQFLQPLIYIPEIQSLIWENSCGSGTASI
                     GVFNNYQRNDACKDFTVHQPGGSILVTSKRCHQLGYQTSIKGQVTTVATGKAYIE"
     gene            10901..11719
                     /gene="cntL"
                     /locus_tag="O2701_001389"
     CDS             10901..11719
                     /gene="cntL"
                     /locus_tag="O2701_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-histidine (S)-2-aminobutanoyltransferase CntL"
                     /protein_id="HCZ4293289.1"
                     /translation="MNNFNNEIKLILQQYLEKFEAHYERVLQDDQYIEALETLMDDYS
                     EFILNPIYEQQFNAWRDVEEKAQLIKSLQYITAQCVKQVEVIRARRLLDGQASTTGYF
                     DNIEHCIDEEFGQCSIASNDKLLLVGSGAYPMTLIQVAKETGASVIGIDIDPQAVDLG
                     RRIVNVLAPNEDITITDQKVSELKDIKDVTHIIFSSTIPLKYSILEELYDLTNENVVV
                     AMRFGDGIKAIFNYPSQETAEDKWQCVNKHMRPQQIFDIALYKKAAIKVGITDV"
     gene            11712..13013
                     /gene="cntM"
                     /locus_tag="O2701_001390"
     CDS             11712..13013
                     /gene="cntM"
                     /locus_tag="O2701_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_096659686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine dehydrogenase CntM"
                     /protein_id="HCZ4293290.1"
                     /translation="MSKLLMIGTGPVAIQLANICYLKSDYEIDMVGRASTSEKSKRLY
                     QAYKKAKQFEVKIQNEAHQHLEGKFEINRLYKDVKNVKGEYETVVMACTADAYYDTLQ
                     QLSLETLQSVKHVILISPTFGSQMIVEQFMSKFNKDIEVISFSTYLGDTRIVDKEAPN
                     HVLTTGVKKKLYMGSTHSNSTMCQRISALAEQLKIQLEVVESPLHAETRNSSLYVHPP
                     LFMNDFSLKAIFEGTDVPVYVYKLFPEGPITMTLIREMRLMWKEMMVILQAFRVPSVN
                     LLQFMVKENYPVRPETLDEGDIEHFEILPDILQEYLLYVRYTAILIDPFSQPDENGHY
                     FDFSAVPFKQVYKNEQDVVQIPRMPSEDYYRTAMIQHIGKMLGIKTPMIDQFLTRYEA
                     SCQAYKDMHQDQHLSSQFNTNLFEGDKALVTKFLEINRTLS"
     gene            13156..14754
                     /gene="cntA"
                     /locus_tag="O2701_001391"
     CDS             13156..14754
                     /gene="cntA"
                     /locus_tag="O2701_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine-dependent metal ABC transporter
                     substrate-binding protein CntA"
                     /protein_id="HCZ4293291.1"
                     /translation="MRKLTKMSAMLLASGLILTGCGGNKGLEEKKENKQLTYTTVKDI
                     GDMNPHVYGGSMSAESMIYEPLVRNTKDGIKPLLAKKWDVSEDGKTYTFHLRDDVKFH
                     DGTPFDADAVKKNIDAVQENKKLHSWLKISTLIDNVKVKDKYTVELNLKEAYQPALAE
                     LAMPRPYVFVSPKDFKNGTTKDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSK
                     LNKVQAKVMPAGETAFLSMKKGETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQP
                     MNTKMLVVNSGKKDNAVSDKTVRQAIGHMVNRDKIAKEILDGQEKPATQLFAKNVTDI
                     NFDMPTRKYDLKKAESLLDEAGWKKGKDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEY
                     LQAEFKKMGIKLNINGETSDKIAERRTSGDYDLMFNQTWGLLYDPQSTIAAFKAKNGY
                     ESATSGIENKDKIYNSIDDAFKIQNGKERSDAYKNILKQIDDEGIFIPISHGSMTVVA
                     PKDLEKVSFTQSQYELPFNEMQYK"
     gene            14767..15702
                     /locus_tag="O2701_001392"
     CDS             14767..15702
                     /locus_tag="O2701_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4293292.1"
                     /translation="MFKFILKRIALMFPLMIVVSFMTFLLTYITNENPAVTILHAQGT
                     PNVTPELIAETNEKYGFNDPLLIQYKNWLLEAMQFNFGTSYITGDPVAERIGPAFMNT
                     LKLTIISSVMVMITSIILGVVSALKRGKFTDRAIRSVAFFLTALPSYWIASILIIYVS
                     VKLNILPTSGLTGPESYILPVIVITIAYAGIYFRNVRRSMVEQLNEDYVLYLRASGVK
                     SITLMLHVLRNALQVAVSIFCMSIPMIMGGLVVIEYIFAWPGLGQLSLKAILEHDFPV
                     IQAYVLIVAVLFIVFNTLADIINALLNPRLREGAR"
     gene            15699..16568
                     /locus_tag="O2701_001393"
     CDS             15699..16568
                     /locus_tag="O2701_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4293293.1"
                     /translation="MIILKRLLQDKGAVIALGIIVLYVFLGLAAPLVTFYDPNHIDTA
                     NKFAGMSFQHLLGTDHLGRDILTRLIYAIRPSLLYVFVALFVSVLIGSILGFLSGYFQ
                     GFVDALIMRACDVMLAFPSYVVTLALIALFGMGAENIIMAFILTRWAWFCRVIRTSVM
                     QYTASDHVRFAKTIGMNDMKIIHKHIMPLTLADIAIISSSSMCSMILQISGFSFLGLG
                     VKAPTAEWGMMLNEARKVMFTHPEMMFAPGIAIVIIVMAFNFLSDALQIAIDPRISSK
                     DKLRSVKKGVVQS"
     gene            16565..17380
                     /locus_tag="O2701_001394"
     CDS             16565..17380
                     /locus_tag="O2701_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4293294.1"
                     /translation="MTLLTVKHLTITDTWTDQPLVSDVNFTLTKGETLGVIGESGSGK
                     SITCKSIIGLNPERLGVTGEIIFDGTSMLSLSESQLKKYRGKDIAMVMQQGSRAFDPS
                     TTVGKQMFETMKVHTSMSTQEIEKTLIEYMDYLSLKDPKRILKSYPYMLSGGMLQRLM
                     IALALALKPKLIIADEPTTALDTITQYDVLEAFIDIKKHFDCAMIFISHDLTVINKIA
                     DRVVVMKNGQLIEQGTRESVLHHPEHVYTKYLLSTKKKINDHFKHVMRGDVHD"
     gene            17373..18122
                     /locus_tag="O2701_001395"
     CDS             17373..18122
                     /locus_tag="O2701_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4293295.1"
                     /translation="MIKIKDVEKSYQSAHVFKRRRTPIVKGVSFECPIGATIAIIGES
                     GSGKSTLSRMILGIEKPDKGCVTLNDQPMHKKKVRRHQIGAVFQDYTSSLHPFQTVRE
                     ILFEVMCQCDGQPKEVMEVQAITLLEEVGLSKAYMDKYPNMLSGGEAQRVAIARAICI
                     NPKYILFDEAISSLDMSIQTQILDLLIHLRETRQLSYIFITHDIQAATYLCDQLIIFK
                     NGKIEEQIPTSALHKSDNAYTRELIEKQLSF"
     gene            18134..19327
                     /locus_tag="O2701_001396"
     CDS             18134..19327
                     /locus_tag="O2701_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ4293296.1"
                     /translation="MKGAMAWPFLRLYILTLMFFSANAILNVFIPLRGHDLGATNTVI
                     GIVMGAYMLTAMVFRPWAGQIIARVGPIKVLRIILIINAIALIIYGFTGLEGYFVARV
                     MQGVCTAFFSMSLQLGIIDALPEEHRSEGVSLYSLFSTIPNLIGPLVAVGIWNANNIS
                     LFAIVIIFIALTTTFFGYRVTFAEQEPDTSDKIEKMPFNAVTVFAQFFKNKELLNSGI
                     IMIVASIVFGAVSTFVPLYTVSLGFANAGIFLTIQAIAVVAARFYLRKYIPSDGMWHP
                     KYMVSVLSLLVIASFVVAFGPQVGAIIFYGSAILIGMTQAMVYPTLTSYLSFVLPKVG
                     RNMLLGLFIACADLGISLGGALMGPISDLVGFKWMYLICGMLVIVIMIMSFLKKPTPR
                     PASSL"
     gene            complement(19551..20300)
                     /locus_tag="O2701_001397"
     CDS             complement(19551..20300)
                     /locus_tag="O2701_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293297.1"
                     /translation="MSNTNKHYIEEEYATEQSRFFKRDIGFIFLYIFLVNILPIIIVF
                     LTFGLVAVFTQQAPWGFSFPISMNISYLLAVIISLAIFRLMHEDAFIPMIRSQIVASA
                     KYKWHILISFTLSVVLFLLLNHFNIFALNVDSFTVFDIYPLIKGYGSISLITIFSLTI
                     ILNTLICQLIFRHILIQELSRLIPLKITIVLSIILETLFYYPYINTWWEFIPALILAG
                     SATYLYLKSYRNFMVSYFYQLAVILVLHIFM"
     gene            20495..22072
                     /locus_tag="O2701_001398"
     CDS             20495..22072
                     /locus_tag="O2701_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HCZ4293298.1"
                     /translation="MTFLTVMQFIVNIIVVGFMLTVIVIGLIWLIKDKRQSQHSVLRN
                     YPLLARIRYISEKMGPELRQYLFSGDNEGKPFSRNDYKNIVLAGKYNSRMTSFGTTKD
                     YQDGFYIQNTMFPMQRNEISVDNTTLLSTFIYKIANERLFSREEYRVPTKIDPYYLSD
                     DHAIKLGEHLKHPFILKRIVGQSGMSYGALGKNAITALSKGLAKAGTWMNTGEGGLSE
                     YHLKGNGDIIFQIGPGLFGVRDKEGNFSEGLFKEVAQLSNVRAFELKLAQGAKTRGGH
                     MEAEKVNEEIAKIRNVEPYKTINSPNRYEFIHNAEDLIRFVDQLQQLGQKPVGFKIVV
                     SKVSEIETLVRTMVELDKYPSFITIDGGEGGTGATFQELQDGVGLPLFTALPIVSGML
                     EKYGIRDKVKLAASGKLVTPDKIAIALGLGADFVNIARGMMISVGCIMSQQCHMNTCP
                     VGVATTDAKKEKALIVGEKQYRVTNYVTSLHEGLFNIAAAVGVSSPTEITADHIVYRK
                     VDGELQTIHDYKLKLIS"
     gene            complement(22240..22695)
                     /locus_tag="O2701_001399"
     CDS             complement(22240..22695)
                     /locus_tag="O2701_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1307 domain-containing protein"
                     /protein_id="HCZ4293299.1"
                     /translation="MKKLCSLIVVALVCIIALSACGKEQTKTYEGDVSGKHVLTSITY
                     KDDKVLKQSTINTIKYDDLGMDKDEAKKLFAKSESIFKDLKGVKYKVDYKDKKAIEHL
                     DIDYTEVDMKKLNKRLGVSTKENKDISFEKLEKQLKHRGLKEKDKMDDK"
BASE COUNT         7986 a         3336 c         4270 g         7129 t
ORIGIN      
        1 atttcaaacc tactgaatag ggttaataga agtttgaaat gaaaatttca taaagtgttc
       61 aaaataattt ttcgctttag ttgtgtaaat taaagattga gtcagtatta aaaatataag
      121 gtggagaaat gatgattcat tcaaagaaat taacgctcgg tatatgcttg gtattactca
      181 ttatattgat tgtaggttat gtcattatga caaaaacaaa tggtcgaaac gcccaaatta
      241 aagacacatt taatcaaaca ttaaaattat atccaaccaa aaatctcgat gatttttacg
      301 ataaagaagg ctttcgagat caagaattta aaaagggtga taaaggtact tggatagtta
      361 attctgaaat ggtaatcgag ccaaaaggta aggatatgga aacgagagga atggtgctct
      421 atatcaatcg caatactaga accacaaaag ggtattattt tataagtgaa atgacagatg
      481 acagtaacgg cagaccaaag gatgatgaaa aaaggtatcc ggtaaaaatg gaacataata
      541 aaatcatacc aacgaagcca ctaccgaatg acaagttaaa aaaagagatt gaaaacttta
      601 agttctttgt acaatatggc aactttaaag atattaatga ttataaagat ggtgatattt
      661 catataatcc taatgtacca agttattcgg caaaatatca attgaataat gatgattata
      721 atgtccaaca gttaagaaaa agatatgata ttccaaccaa acaagcgccg aaactattat
      781 tgaaaggcga tggagattta aaaggttcat ccgtaggttc tagaagtctt gaatttacct
      841 ttgtcgaaaa taaagaagaa aacatttatt ttacagattc tgtacaatat actccaagtg
      901 aggatacaag gtatgagtca aactgaatat caaataaaac ctggtaatat aacaagtaac
      961 tctgaagaaa caagttcgat atctaaagtg agctgtgaaa tataggtagc catttcaaaa
     1021 aatttaaagg tgaatttgat aatgtagctc aaggagattg ggttaaaaag gcgaagaatg
     1081 aactggatga tattagtaag aaattaaaaa atattcaaag aacggaagtt taatagctta
     1141 tatgattctt ggagctaaga cagcatgcgt tcattcatgc cattattaat ataagcaccg
     1201 caacaaaaaa gcttctaatg tgatacagga acctcatatt ccgtatcatg ttagaagctt
     1261 ttaatgtcta aagaacatct acattttatc atattttctg acttattaaa cttttatata
     1321 attaaatatt tcttaatttt ccaaaatagt gataaatttg tgaaatacat cacaaatccc
     1381 tttatttatt tggaaattca tgtaatatta gacttgtaag aagttaataa atagagagag
     1441 acgagagagt ttatataaat actatataaa cattggagtg atgattatga gaaaagagat
     1501 tgaagcgctt attttctcag acgtaagtag ctatgatatt tacgtgaaca ctggtgtaaa
     1561 tcaaggatta attggtgaca tcaaagatgg ttacctaact attgattcta tgccttacat
     1621 tgatgctgag cgtttgtatc actttgctat ggaacgtaaa tcgttagtca ctaactagtt
     1681 cttattgcca atgattacta cccctagtcg gcggcaattg aagtgtgatt gatgtaactt
     1741 gccctcgttg gtgagcaatt gagggcagac ccctttaatt aagtaaaccc taactcccca
     1801 caaatctgga acgatactaa aagccacgtc ctatattgga tgtggcttta gtcatactta
     1861 tattattttt aaaacgatta cctacaagat ttacatataa aattctatca tgtctgctat
     1921 atataaacga aatgatatta gaatccttca ttttcaactt gtttaaaatt acgtttaatg
     1981 ccgttataag aatatacaac attatataat tttacttggg cgtcatggac acttgagaag
     2041 ggcattctta catcgtccat atttttaact tcgtgtcgaa atgctttttc agctttttgt
     2101 ctttctgcaa gagacatact tagccattct tctttgctaa ataaatcttc gaatcgaaaa
     2161 tcaaatgcgg ttcttttact ttctgctctt aatgctaaaa cacgctcatg atatgtcacg
     2221 ttttattcct ccctctaatc attcaatcga atgataaatc acagtattaa ctttattact
     2281 aatataatgt ttttacaata aagaagtgac gatttttcaa catttttaaa aataaaaaat
     2341 ataagcaacg attaattgta tgtttcgaat ggcttgtaat gtaatttatc tatgaaaatg
     2401 aagcgagtta ttatgaccgt tatatgaatt gtgatacgct ttttaaaagt aatatgtatt
     2461 tatgatttac cctgaaaaaa taaaaagaat caaaggagtc gatcattatg acagtattaa
     2521 cagataaagt agcagtagtt acaggtgcag gtagtggtat tggagaagca attgcaacat
     2581 tactacatga agaaggggca aaagttgtct tagcaggtag aaataaagaa aaattacaaa
     2641 acgtagcgaa tcaattgtca caagatagtg tgaaggtagt gccaacagat gtaacgaata
     2701 aagaagaagt cgatgaattg ataaaaattg cacaacaaac attcggtggt ttggatattg
     2761 ttatcaatag tgcggggcaa atgttgtcgt ctaagattac tgattatcaa gtagatgagt
     2821 gggatagtat gattgatgtg aatatcaaag gcactttata tacggcacag gctgcattac
     2881 caactatgtt agaacaatca agtggccatc ttattaacat tgcatctatt tctggctttg
     2941 aagtaacgaa aagtagtacg atttatagtg cgacgaaagc agcagttcac actattactc
     3001 aaggattaga aaaagagttg gcaaagacag gcgttaaagt aacaagcatt tctccaggaa
     3061 tggtagatac agccataact gccgcataca atccaacaga tcgtaaaaaa cttgaaccac
     3121 aagatattgc agaagcagta ttatatgcat taacacaacc aaagcacgtt aacgtgaatg
     3181 aaattacagt gcgtcctgta taaaaaggtg tttgaggtct ggggtatagg catttaatgt
     3241 aatagctatc gattttgtta atgttagtaa ggcaataatt acatgagggc ataataggca
     3301 attattcctt gtacaattct cagtaattgc tgattttaac aatcaagtta atgaaggcga
     3361 cgatgttagt gttatggttt ataatctgta atgttcataa ttatagatta taaaaatttt
     3421 ttcattttcc accacatttt aggagttact ttaatgaaca aaaaacatgt ttttataatt
     3481 attggtgtca ttttatgtat atgtatagtt gcatcagtca tttatttaaa agtgaaatat
     3541 gacgaaaaag aaaaacaaaa agcaatatac tataaagaac aacaggagcg tattacactc
     3601 tatcttaagc ataatactaa agaacccaat accatcaaaa ctgtacattt cacaagttta
     3661 aaaagaggac ctatgggcga tgcagtaatt gaaggttaca taaatgaaaa taaagaagat
     3721 gattttgttg catatgggtc tccagaacat aattatcaat ttggtggaag tttaatcaaa
     3781 agtaaaaatt taagcacgtt attaaaacca gtacatcaaa ccaaatcacc tgatgagata
     3841 aaaaaggaat tagagtctaa aaagaacgac cgctaaaact atgcggccgt tttaattttg
     3901 ttttcaagtt aatcatactt acatataaaa atgaaaagtt gaatttttag tacaatttcc
     3961 actgtattta tctgttaacc actttatacc cgcaatattt cgttgatctt atataaacac
     4021 ttttggctgc ttttccttga cctagattaa aattatagat tttctttgta agagtgtgtt
     4081 ttgtagtatc ataaatatgt aaactgtata aaattccacc acattttagg agctggtgat
     4141 agatgtcgaa aaagaaaatt ttaatattaa ctagtattat gttaatcata ttaattagtg
     4201 ttgcaggtat ctatttaaaa gtgaaatatg acgaaaaaga aaaacaaaaa gcaatatact
     4261 ataaagaaca acaagcgcgc attacacttt atctcaagca taacactaaa gaaccgaata
     4321 caatcaaatc tgtacatttc acaaactcgg aaacaagtcc tatgggaagt gctgtgattg
     4381 aaggttacat aaatgaaaac aaaaaagata attttactgc ttatgctaca ccagaacata
     4441 attatcaatt tggtggtgct atgatagaaa gtgaaaaatt aagcgagtta ctaaagccag
     4501 ccaatcagtt aaaatcacca gatgatataa aaaaagaact aaataaaaag aagagtcact
     4561 aaagttagga gttactttaa tgtccaaaaa acatgttttt ataattattg gtgtcatatt
     4621 gtgtatatgt acagtttcta cggtcatgca ttttaaaatg aaatatgatg aaaaagaaaa
     4681 acaaaaagcg atttactaca aagaacaaca agaacgtatt acactctatc ttaagcataa
     4741 tactaaagaa acgaacacga ttaaatctgt acatttcaca aacttggaaa caagtcctat
     4801 gggaagtgct gtgattgaag gatacatcaa tgaaaataaa gaagatgatt ttactgctta
     4861 tgcatcgcct gaacataatt atcaatttgg tggcgctatg ataaaaagtg aaggagtaga
     4921 taaattatta aaaccagcac atgaaagaaa atcaccagaa aaaatcaaag aagaattaga
     4981 taaaaaagaa ggccactagg gtcttcttta tttttgattt aatcttccaa taatctatgt
     5041 cattgctatc gaaggtgttt cgcaattaat ataaatcact tcatcatcac caatacttcc
     5101 ccagttttgt acagtacatt aacacaaacg aaccacgtta atttaaatgg aataatagtt
     5161 tggccattat aagaacaata tatatcgaat aacaatgtcg atgacgtgat ttattgacat
     5221 tatcaaattt tctcaaatgt gtgtgatttt ttataaaaaa gggtagagca aggtataata
     5281 tcctttcaat ctgttttcat attttatata ttttttaaat ataagtgcta aatgttttaa
     5341 ctaaagcata gattgacaag atgttataca gaatttcaaa ttctatccaa tattgttcga
     5401 agtgtagtat cactggattg gtattaaaca atgtaaagga gagattgcaa atgccgtata
     5461 attacaagaa acaaaatgga gagttaatgt ctgtaatgag ccaaggtgaa aagtttattc
     5521 atcaatcacc cgttaatgat gaacttagtg cattgattaa gttattaatt tctaaaatta
     5581 acggttgtca ttattgtgtt gatatccata aaaaagaatt aaaggaattg ggtgtaacac
     5641 aaatgaaaat tgatgaagtc ttgagtttta gacatttaga tttatttact gatcaagaaa
     5701 aagtgacgct tgaatttgca gaaatgttaa attcaatcaa agactttaag aagtttgaaa
     5761 ttattgaccg gctaaaatca ttttatgatg aagaacaaat tattgatctt gtctttgttg
     5821 taaaccaaat taacggttgg aacagattaa atattattag tgatagacta taattgttca
     5881 tataaatgca gagtttcatc tcgaacgcta tatcataaca aatcatgcca ctatacaggt
     5941 caaatcttgt atagtggcat tttaatttat ccctttgaat actgttattt aacgaatatc
     6001 ggtccacctg gtccaacagg gatacctaat aggaaccaaa tgatgacaaa tactgtccat
     6061 acaatactta gcgcgattga atacggcatt aaactagaaa gtaaggctcc gagtttcatg
     6121 cgtttatcgt atttttgtgc ataagttaat aataaaggta agtacggcat catcggtgta
     6181 attggattgg taattgaatc gcctacacgg taaatgactt gtgtgaatgc gggatgaaag
     6241 ccgataagga ttaacattgg tacgaatatc ggtcctaaaa taccccattt agccgatgcg
     6301 cttccgatta acatgttgac cattgcactc agtacaataa tacctagtat caatacaata
     6361 ccgttttgat gttctaataa tttggcacct ttaacagcag cgataattcc taaattactc
     6421 cactttaaat acgcaagtag ctgtgctgca aaaaacacaa taacgataaa tgttcccatt
     6481 gatcctacag catcgccgaa cattttacct aagtcttttg tatttttaat ttctttgctt
     6541 aaaatcccat aaactaatcc aggtactaaa aatacgacaa gaataattaa tccgacaccg
     6601 ttaattaatg gcgcatcgtc tagtaagctg cctgttttag catttcttaa aaagctatgt
     6661 tcaggaatgg ctgtaataat taataaaata attgtgacta tgaaactgat atttgcccat
     6721 tttaaagcat gtgcttcttt gtcagtaata tgtgaagatg tttcttcatg gtcatcatgc
     6781 attaaactgt catcatattt tcctaatcta ggaataatga gcttagttgt aactagcaat
     6841 atcgtaggaa gtaatacaac gacactcgct gcgataaagt accagttcat agcaacgttt
     6901 gttttaatag aatctgaaac gatacgtgtt gccggttctg taaatgaata gaccaaagca
     6961 tcttgcatac caacaactat atttgctgca aatcctccaa cagcggaagc atatgccatc
     7021 gttagtccag cgatagggtg atagccaatt ttaataaaaa gcattgctgc aagcggcggc
     7081 aagataattg tcgcagcatc gccggctgta ctacctaaaa taccaattaa tataatagtc
     7141 ggtaaaatta agaagcgtgg tgcgcgattc acaacagaaa tcattaactt atcgaagtat
     7201 cctgttttct ctgcaacacc aataccaatc atcactgcta gtactaagcc taatgctggg
     7261 aactctgaga aatttttaat cgtatcattc attatcatcg taaatccatc atggctaatt
     7321 atatttttaa tataaatggt ttgatgcgta cctggatgct taacagatac attaaataat
     7381 gagataaccc atgtcataat ggctaagccc acacacatta aaaagaataa gacgctagga
     7441 tctggcaatt tatttccgat tttttcaaca ctattcaaga aacgattgac gatagacccc
     7501 ttttgttgat gttttgatgt catcaattat tccccccttt gttaaatatt taaagtgtaa
     7561 caaaaaatac tctcaaaagt aacaattttc agaaaataaa aaaactaata ttgttaaata
     7621 ttttgagtta ttcaatagaa agtgtatagc agagtagtta agactgcctg aagacttatc
     7681 tattaggttt atgaagcatc gaacagtgga aaataaggac tgtaagttta agatatgttg
     7741 tataggagtg actgaatgaa acgtttggaa aataaagtag cagtcgtaac aggagcaagt
     7801 acaggtatcg gtcaagcttc tgcaatcgct ttagctcaat aaggtgcgta tgtattggcg
     7861 gtagacatag ctgaagcggt atcggagact gtcgataaaa ttaaaagtaa tggtgacaat
     7921 gcgaaggcgt ataatgtgga taatgcaagc gaacaacaag tggtagactt tgtgtctgac
     7981 ataaaggaac agtttggaag aatcgatgtg ttgtttaata atgccggtgt ggataatgcg
     8041 gctgatagaa ttcatgagta tccaatagat gtgtatgaca agattatgaa tgtagatatg
     8101 cgtgggacat ttttaatgac gaaaatgatg ttacctttaa tgatgaatca aggtggctct
     8161 attgttaata cgtcttcatt ttccggacaa gcagcagact tgtatcgctc tggatataat
     8221 gctgcgaaag gtgcagtgat taattttaca aaatcaatcg caattgagta tggccgtgat
     8281 agcattcgag ccaatgcgat tgcaccaggt acaattgaaa ccccgttagt agataaactg
     8341 acaggtacga gtgaggatga tgcaggtaaa acatttagag aaaatcaaaa atggatgact
     8401 ccgctgggac gtttaggtaa accagaagaa gttgctaaat tagtagtctt cttagcatct
     8461 gacgacagtt cattcataac tggagagacg attcgaattg atggtggtgt gatggcttac
     8521 acatggcctg gcgagatgtt aagtgatgat tcgtggaagc ggacattgga ataattattt
     8581 aaaatcgata tccagtggaa aagaatttgg catgaagtag gcaatgtatg catattgtcg
     8641 caatgaaaga catttaaagc ggagattaat taatctttga aaatcacata ttgttcattt
     8701 gaagtgtatt gcttaaagct tgttttaacg tattgtaggt cgtgcatcta atttatcaga
     8761 atctcgagtt caaacttttg gagtatcttt aaaataagct gtattttatc tattctaata
     8821 aattaaggag aattttatgt taaaagaaaa agaaagtttt agattgctat atcaagctat
     8881 aagagagatt gcagataaaa ttggagataa tcagttagaa actaattccg ttagtttatt
     8941 attattggac tttgattttg aacatgaagt atttgatgaa ttgtatcttg tgattttaaa
     9001 atatttaaat acagtaagta tagagaacat aagtcatagt gagcttttga atttaataga
     9061 aaatacgatt ccagaagatc gagagattaa cacgtttgta aaaaataaaa taataattgg
     9121 ctttgctaat aattacttcc ctgaattaca agtattagca aacgaaataa aatctgatat
     9181 ggctagttca ttaaaacaat gatattttta tttaaatttt taaagctttg tacgaaattg
     9241 tacaaagctt ttttggtgcg tattgtatgg gcaacaactt gacgatgaaa atccgttaca
     9301 ggattggtaa taggaaatgt tagcgaaaga caagggtatc cattgtagat taacaaaagg
     9361 acgtttccac aagtgtgggt tattctcact aaagcaatac gcagagacaa cttacgtaaa
     9421 attttgaact gactagaacg gaacttctac tcaattattg ataaaaattt tcaaaaagac
     9481 ttgaatgtgc tgagaatacg aagtttatgg aaggattatc aaaatataaa tgtgcattca
     9541 tttacaacct ttattgacaa tgattctcaa ctaatatagt atataatcaa atcgtaatag
     9601 ttacgatttg ttttctgcaa cttttttgaa gttttagttg aggtgaaaac agtaaaagca
     9661 tctaagtgaa tgtagttaac ggacaactgc attcgcttgt agagccacaa gaagcaactt
     9721 taaataaggt ttacggttgc attttgatac aacaaccgat tactaagtca tgctttccac
     9781 tttgcgggtt agcatgactt acctaataga tagagctatt aggttcagct tctaaaaaat
     9841 tacagtttta gaggaataca gttgcttgct tcgcaacaac tgcataagag ccatggtttt
     9901 cgcttttgcg aattagcatg acttacctac tagatagagc tattaggttc atcttctaaa
     9961 aaattacagg tttagaggaa tacagttgtt tgcttcgcaa caactgcata agagcctcta
    10021 gtaattaaaa ttacagaggc tctaaaaata catctaaagg agtgtcgtat gaatcggcag
    10081 gttatagaat tttctaagta taatccttcg gggaatatga cgatacttgt tcattcaaaa
    10141 catgatgcta gtgaatatgc atctatcgcc aatcagttga tggccgcaac acatgtatgc
    10201 tgtgaacagg taggctttat agaatcaaca caaaatgatg atggtaatga ttttcactta
    10261 gttatgagcg gtaatgaatt ttgcggtaat gcgacgatgt catatataca tcatttgcag
    10321 gaaagtcatt tgcttaaaga ccaacagttt aaggtgaagg tgtctggctg ttcggattta
    10381 gtgcaatgcg caattcatga ttgccaatac tatgaagttc aaatgccaca agcccatcgt
    10441 gttgtgccaa caacaattaa tatgggtaat cattcatgga aagcaataga aattatttat
    10501 gaaacatatg tacattatgt gattccagtt aaacaagtaa caacagaaat tcaacatttg
    10561 gttgaagcgt ttgtgcgtga gcaacaatgg agtcacaaat ataaaacagt aggtatgatg
    10621 ctttttgatg aacaacgtca atttttacag ccattaatct atataccaga aattcaaagt
    10681 ttaatttggg aaaatagctg tggttctggt acagcatcaa ttggggtttt taataattat
    10741 caacgtaatg acgcatgcaa agattttaca gtacatcagc cagggggcag tattttagtg
    10801 acatcaaagc gatgtcatca attgggatat caaacttcaa ttaaaggaca ggttacaact
    10861 gtagctacag gaaaagcata tatagaataa ggagcctaca atgaataact ttaataatga
    10921 aatcaaattg atattacaac aatatttaga aaagtttgaa gcgcattacg agcgtgtatt
    10981 acaagacgat caatatatcg aagcattaga aacattgatg gatgactata gtgaatttat
    11041 tttaaatcct atttatgaac aacaatttaa tgcttggcgt gacgttgaag aaaaagcaca
    11101 attaataaaa tcactgcaat atattacagc gcagtgtgtt aaacaagtgg aagtcattag
    11161 agcgagacgt ctattagacg gacaggcgtc taccacaggt tactttgaca atatagaaca
    11221 ttgtattgat gaagagtttg gacaatgtag tatagctagc aatgacaaat tattgttagt
    11281 tggttcaggt gcatatccaa tgacgttaat tcaagtagca aaagaaacag gtgcttcagt
    11341 tatcggtatt gatattgatc cacaagccgt tgacctaggg cgcagaatcg ttaacgtctt
    11401 agcaccaaat gaagatataa caattacgga tcaaaaggta tctgaactta aagatatcaa
    11461 agatgtgacg catatcatat tcagctcgac aattccttta aagtacagca ttttagaaga
    11521 attatatgat ttaacaaatg aaaatgtcgt agttgcaatg cgctttggtg atggcatcaa
    11581 agcaatattt aattatccgt cacaagaaac agcggaagat aagtggcaat gtgtgaataa
    11641 acatatgaga ccacagcaaa tttttgatat agcactttat aaaaaagcag ctataaaggt
    11701 aggtattacg gatgtctaaa ttattaatga taggcactgg tccggtcgca atccaattag
    11761 cgaatatttg ctatttaaaa tcagattatg agattgatat ggttggacgt gcctcaacat
    11821 cagaaaaatc aaaacgctta tatcaagcgt ataaaaaagc gaaacaattt gaagtcaaaa
    11881 tacaaaacga ggcgcatcaa catctggaag gtaagtttga aattaatcgt ttgtataaag
    11941 atgttaaaaa cgttaagggt gaatacgaaa cggttgtcat ggcatgcaca gcagatgctt
    12001 attatgacac actacagcaa ttgtcgttag aaactttgca aagtgtcaaa catgtcattt
    12061 taatatcacc gacatttggt tcgcaaatga ttgtcgaaca atttatgtct aaatttaata
    12121 aagatatcga agtgatttca ttctcaactt atcttggcga tacacgtatt gttgataaag
    12181 aagcgcctaa tcatgtgttg acaacaggtg taaaaaagaa attgtacatg ggatcgacac
    12241 attcaaactc aacaatgtgt caacgaatct ctgctttagc tgagcaattg aagattcaat
    12301 tagaagtcgt tgaatcgcca ctgcatgctg aaacgcgaaa cagttcgctt tatgtgcacc
    12361 caccactatt tatgaatgac ttttcattga aagccatttt cgaaggaaca gatgtaccgg
    12421 tttatgtgta taagttattt cctgaaggac cgataacgat gacactaatc cgtgaaatgc
    12481 gtttaatgtg gaaggaaatg atggttattt tacaagcatt tagagtgccg tcagtcaacc
    12541 tgcttcaatt tatggtgaag gaaaattatc cagtacgtcc tgaaactttg gatgaaggtg
    12601 atattgagca tttcgaaatc ttgccagata tcttacaaga atatctgctt tatgtaagat
    12661 ataccgcaat cctcattgat ccattttcac agccagacga aaacggacat tactttgatt
    12721 tttcagctgt accatttaag caagtctata aaaatgaaca ggatgttgtt caaattccaa
    12781 gaatgccaag tgaagattat tacagaacgg cgatgattca gcatattggg aaaatgctag
    12841 gtatcaaaac gccaatgatt gatcagttcc taactcgcta tgaagcaagt tgccaggcgt
    12901 acaaggatat gcatcaagat caacacttat cttctcaatt taatacaaat ctatttgaag
    12961 gagataaagc actcgtcaca aaatttttgg aaatcaatag aacgctttca taataagggt
    13021 ttgaagtttt ataatagaaa aaaattattg aattatgttt gacatttaca taaaaataag
    13081 caaataattg agaaaaataa tcattacgat ttgattaagt aatgcaactt atcaatttag
    13141 aaagaggaaa agcaaatgag aaaactaact aaaatgagtg caatgttact tgcatcaggg
    13201 ctaattttaa ctggttgtgg cggtaataaa ggtttagagg agaaaaaaga aaacaagcaa
    13261 ttaacgtata cgacggttaa agatatcggt gatatgaatc cgcatgttta cggtggatca
    13321 atgtctgctg aaagtatgat atacgagccg cttgtacgta acacgaaaga tggtattaag
    13381 cctttactag ctaaaaagtg ggatgtgtct gaagatggga agacatacac gttccatttg
    13441 agagatgacg ttaaattcca tgatggtacg ccatttgatg ctgacgcagt taagaaaaat
    13501 attgacgcag ttcaagaaaa caaaaaattg cattcttggt taaagatttc gacattaatt
    13561 gacaatgtta aagttaaaga taagtacacg gttgaattga atttgaaaga agcatatcaa
    13621 cctgcattgg ctgaattagc gatgcctcgt ccatatgtat ttgtgtctcc aaaagacttt
    13681 aaaaacggta caacaaaaga tggcgttaaa aagttcgatg gtactggtcc atttaaatta
    13741 ggtgaacaca aaaaagatga gtctgcagac tttaacaaaa atgatcaata ctggggcgaa
    13801 aagtctaaac ttaacaaagt acaagcaaaa gtaatgcctg ctggtgaaac agcattccta
    13861 tcaatgaaaa aaggtgaaac gaactttgcc ttcacagatg atagaggtac agatagctta
    13921 gacaaagact ctttaaaaca attgaaagat acaggtgact atcaagttaa gcgtagtcaa
    13981 cctatgaata cgaaaatgtt agttgtcaat tctggtaaaa aagataacgc tgtgagtgac
    14041 aaaacagtca gacaagcgat tggtcatatg gtaaacagag ataaaattgc caaagaaatt
    14101 ttagatggtc aagaaaaacc agcaactcaa ttatttgcga aaaatgtaac agacattaat
    14161 ttcgatatgc caacacgtaa gtatgacctt aaaaaagcag aatcattatt agatgaagct
    14221 ggttggaaga aaggtaaaga cagcgatgtt cgtcaaaaag atggtaaaaa ccttgaaatg
    14281 gcaatgtact atgacaaagg ttcttcaagt caaaaagaac aagcagaata cttacaagca
    14341 gaatttaaga aaatgggtat taagttaaac atcaatggcg aaacatcaga taaaattgct
    14401 gaacgtcgta cttctggtga ttatgactta atgttcaacc aaacttgggg attattgtac
    14461 gatccacaaa gtactattgc agcatttaaa gcgaaaaatg gttatgaaag tgcaacatca
    14521 ggcattgaga acaaagataa aatatacaac agcattgatg acgcatttaa aatccaaaac
    14581 ggtaaagagc gttcagacgc ttataaaaac attttgaaac aaattgatga tgaaggtatc
    14641 tttatcccta tttcacacgg tagtatgaca gttgttgcac caaaagattt agaaaaagta
    14701 tcattcacac aatcacagta tgaattacca ttcaatgaaa tgcagtataa ataaaggagc
    14761 aattagatgt tcaaatttat cttaaaacgt attgcgctca tgtttccatt gatgattgta
    14821 gtaagtttta tgacatttct attgacgtat attacaaatg aaaatccagc tgtgacaatt
    14881 ttacatgcac aagggacgcc aaatgtaaca ccagagttga ttgcagaaac gaatgagaag
    14941 tacggtttca atgatccatt attaattcaa tataaaaatt ggttacttga agcgatgcaa
    15001 tttaattttg gtacaagcta cattacaggt gacccagttg ctgaacgtat tggtccagca
    15061 tttatgaata cattgaaatt aacaataatt tcaagtgtta tggtgatgat tacatcaatt
    15121 attttaggtg tagttagtgc attaaaaaga ggaaagttca ctgatcgtgc gatacgttca
    15181 gtggctttct ttctaactgc attaccatca tattggatag cttcaatact tattatttac
    15241 gtttcagtga agttaaacat attgccgact tctggattaa caggtccaga aagttacata
    15301 ttgccagtga tcgttattac gattgcctat gctggtattt actttagaaa tgttagacgc
    15361 tcgatggtgg aacaattaaa tgaagattat gtactttatt taagagcaag cggtgtgaaa
    15421 tctatcacat taatgttgca tgtgttgcgt aatgctttac aagttgcggt atcaatcttt
    15481 tgtatgtcta taccaatgat aatgggtgga ctagttgtta tcgagtatat ctttgcatgg
    15541 cctggactag gtcaattaag tttaaaagca atacttgaac acgattttcc agtcattcaa
    15601 gcatatgtat taattgtagc ggtattattt attgtattta atacattagc agatatcatt
    15661 aatgcgctat taaatccaag attaagggag ggcgcacgat gataatttta aaacgattat
    15721 tacaagataa aggtgcagta attgctttag gcattattgt attatatgtc tttttaggat
    15781 tagcagcacc acttgtgaca ttttatgatc ctaaccatat cgatacagca aacaaatttg
    15841 ctggcatgag ttttcaacat ctactaggta ctgaccattt aggtagagat attttaacta
    15901 ggttaattta tgcgattaga ccaagtttgt tatatgtctt tgttgcgcta tttgtttctg
    15961 tacttattgg atctatttta ggattcttat caggatattt ccaagggttt gttgacgcct
    16021 taatcatgcg tgcgtgtgat gttatgttgg cattcccaag ttatgttgta acgttagcat
    16081 taattgcatt gtttggaatg ggtgccgaaa atattatcat ggcatttatt ttgacgcgtt
    16141 gggcatggtt ctgtcgtgtt atacgtacaa gtgttatgca gtacactgct tctgaccatg
    16201 taagatttgc taaaacaatc ggtatgaatg atatgaaaat tattcacaaa catattatgc
    16261 cattaacatt agcagatatt gctatcatct ctagtagctc gatgtgttca atgatcttgc
    16321 aaatatctgg cttttcattt ttaggattag gtgtcaaagc gcctactgca gagtggggca
    16381 tgatgcttaa cgaagctaga aaagtgatgt ttacacatcc tgaaatgatg tttgcgccag
    16441 gtattgccat agtgattata gtgatggcat ttaacttctt atccgatgct ttacaaattg
    16501 ctattgatcc ccgcatctct tctaaagata aacttcgttc tgtgaaaaaa ggagtggtgc
    16561 aatcatgaca ttgttaacag ttaaacattt gacgattaca gatacctgga cagatcaacc
    16621 actcgtgagt gatgtgaatt ttacattaac taagggtgaa actttaggcg ttattggaga
    16681 aagtggtagt ggtaaatcaa tcacttgtaa atcgattatt ggtttgaatc ccgaacgact
    16741 cggggtgaca ggtgaaatta tctttgatgg tacatcaatg ttgtcattat ctgaatcgca
    16801 attgaaaaag taccgtggta aagacattgc gatggtcatg caacaaggta gtcgtgcctt
    16861 tgacccatca actactgtcg gtaaacaaat gtttgagact atgaaagtac atacgtcaat
    16921 gtctacacaa gaaattgaaa agacattgat tgaatatatg gattatttaa gtttgaaaga
    16981 tcctaaacgt atattaaaat cataccctta catgttatca ggaggaatgt tacagcgatt
    17041 gatgattgct ttagcgttag ctttgaaacc aaagttaatc attgctgatg agccgacaac
    17101 ggctttagat acaattacac aatatgatgt actggaagca tttatagata ttaaaaaaca
    17161 ctttgactgt gcgatgattt tcatttcaca tgatttaacg gttattaaca agattgcaga
    17221 ccgtgttgtt gtgatgaaaa atggtcagct tattgaacaa gggacacgtg aatcagtctt
    17281 gcatcatcca gaacatgttt atacgaagta tttattatca acgaagaaga agattaatga
    17341 tcattttaaa catgtgatga ggggtgatgt acatgattaa aattaaagat gttgaaaagt
    17401 catatcaaag cgcacatgtt tttaagcgtc gtcgaacacc tatcgtgaaa ggtgtgtcat
    17461 ttgagtgtcc aatcggtgcg acgattgcga ttatcggaga aagtggtagc ggtaaatcga
    17521 cgttgagtcg tatgatatta ggtattgaga aaccggataa aggttgtgta accttaaatg
    17581 atcaaccgat gcataagaag aaagtgagac gtcatcaaat tggtgctgta tttcaagatt
    17641 atacgtcatc attacatcca tttcagactg ttagagaaat cttatttgaa gtgatgtgtc
    17701 aatgtgatgg acaacctaaa gaagttatgg aagtccaagc aattacattg ttggaagaag
    17761 tcggtctatc taaggcatac atggataaat atcctaatat gttatcaggt ggagaggcgc
    17821 aacgtgttgc gattgcgcgt gcaatatgta ttaaccctaa atatattttg tttgatgaag
    17881 ccattagttc actcgacatg tcaattcaaa cacaaatatt agatttattg attcatttac
    17941 gtgaaacgcg tcagttgagt tatattttta tcacacatga tattcaagct gccacgtatt
    18001 tatgtgatca attaattatt tttaaaaacg gaaaaataga agaacaaatt ccgacaagcg
    18061 cattgcataa aagtgacaat gcttatacaa gagaattaat agaaaaacaa ctatcattct
    18121 aaggagtgag ataatgaaag gtgcaatggc ttggcccttt ttgagattat atatattaac
    18181 attgatgttc tttagtgcca atgcaatctt aaacgtgttt atacctttac gagggcatga
    18241 tttaggcgca acgaatacgg ttatcggtat cgttatgggg gcatacatgt taacagcaat
    18301 ggtatttcga ccatgggcag gacaaattat tgctcgtgtc ggtcccatta aagtattaag
    18361 aattattttg attatcaatg ccatagcttt aattatttat ggttttactg gcttagaagg
    18421 ttatttcgta gcacgtgtta tgcaaggtgt gtgtacggca ttcttttcta tgtctttaca
    18481 gctaggtatt attgatgcat taccagagga acatcgttct gaaggtgtat cattgtactc
    18541 gctattttca acgattccaa acttaatcgg accattagtt gccgtaggta tttggaatgc
    18601 aaataatatt tcactatttg caattgtcat tatctttatc gcattaacaa caacattctt
    18661 tggctatcgc gtgacctttg ctgaacagga acccgatacg tcagataaga ttgaaaaaat
    18721 gccgtttaac gctgtaactg tttttgcgca atttttcaaa aataaagagt tgttgaacag
    18781 tggtattatc atgattgttg catcgattgt atttggtgca gttagtacat ttgtaccgtt
    18841 atacacagtg agtttaggat tcgcgaatgc gggaatcttt ttgacaatac aggccatcgc
    18901 agttgttgcg gcaagatttt acttaaggaa atacattccg tcagatggta tgtggcatcc
    18961 taaatatatg gtatctgtac tatcattatt agtaatcgcg tcatttgtag tggcatttgg
    19021 tccgcaagta ggtgcaatta ttttctatgg tagtgcgata ttaataggaa tgacgcaagc
    19081 aatggtgtac ccaacattaa catcatactt aagcttcgtc ttaccaaaag taggtcgtaa
    19141 tatgttgtta ggtttattta ttgcctgtgc agacttaggt atatcgttag gtggcgcatt
    19201 gatgggacct atttccgatt tagtaggatt taaatggatg tatctaattt gtggtatgtt
    19261 agtcattgta ataatgatta tgagtttctt gaaaaagcca acaccacgtc cagcgagtag
    19321 tctttaatga agtgaattaa agcatattaa gttaatgaat atttaaattt taaaaggtat
    19381 attgagcatg gcgattcatg tgcttcatgc taggacatga aacattctat atggctcgtt
    19441 tttagaacga cagtatatct aaataaagca cgcttaaaag tgagttttga tactgacttt
    19501 taagtgtgct ttttgatgtg tatggataat ggataatact ctgaaaaatt ttacatgaaa
    19561 atatgcaaaa cgagtataac tgctaattga tagaaatagc tcaccataaa attacggtat
    19621 gattttaaat ataagtaagt cgcactacct gctagtatca atgctggaat gaattcccac
    19681 catgtattaa tgtatggata gtagaacaga gtttcaagga taatggacaa tactattgta
    19741 atctttaaag gtattaatct gcttaattct tgaattaaaa tatgacggaa aataagttga
    19801 caaatcaaag tatttaatat aatggttaac gaaaatatag ttattaaact gatggaacca
    19861 taccctttaa tgagcgggta aatgtcaaag acagtaaagg aatctacatt tagtgcgaaa
    19921 atattgaaat gatttaaaag taaaaagagt acgacactta gtgtaaatga tataagaata
    19981 tgccatttat atttagcact agcaacgatt tgcgaacgta tcattggaat aaacgcatct
    20041 tcatgcatca gacgaaaaat agctagtgaa ataataactg cgagtaaata gctaatgttc
    20101 attgaaatag gaaaagagaa accccacgga gcttgttgag tgaatacagc tactaaccca
    20161 aaagttaaaa agacgataat gatcggcaag atgttaacca aaaatatgta aaggaaaata
    20221 aatccaatat cacgtttgaa aaaacgcgat tgttcggtag cgtattcttc ttctatgtaa
    20281 tgtttatttg tatttgacat agtatacctc ttaaatagtt gtattatata gatactttag
    20341 cacatattac tttgtattgt atgttttata cattaaaatt taaaatgaaa aacatatcat
    20401 aaaattgttt tataaaatga agcgcttcca ttgtgttttg ttttgtaagg tgtatcataa
    20461 atattgaatt gaaattttgg ggggaggtat tgtaatgacg tttcttacag tcatgcaatt
    20521 tatagttaac attatcgttg taggattcat gcttacggtt attgttatcg ggcttatttg
    20581 gttaattaaa gataaaagac aatcacaaca tagtgtatta aggaattatc ctttactagc
    20641 acgtattaga tatatttcag aaaaaatggg accggaatta cgtcagtatt tattttctgg
    20701 ggataatgaa gggaaacctt tttcacgtaa tgattataaa aatatcgttt tggctggaaa
    20761 atataactct cgtatgacca gcttcggtac tactaaagat tatcaagacg gcttttacat
    20821 acagaacaca atgtttccga tgcaacgtaa tgagatttca gtagataata caacattgtt
    20881 atcaacattc atttataaaa tcgcgaatga gcgtttattt agtcgtgaag aatatcgtgt
    20941 gccgacaaag attgatccgt attacttaag tgatgaccat gcaataaaat taggtgaaca
    21001 tttaaaacat ccatttattt taaaacgtat cgtaggacaa tctggtatga gttatggcgc
    21061 tttaggaaaa aatgccatta cagctttatc taaaggtcta gctaaagcgg gcacttggat
    21121 gaatacaggt gaaggtggct tatcagaata tcatttaaaa ggtaatgggg atatcatttt
    21181 ccaaattggt cccggtttat ttggtgttcg tgataaagaa ggtaatttta gtgaaggttt
    21241 atttaaagag gttgcacagt tatctaacgt acgcgcattt gagctgaagt tggcacaagg
    21301 tgctaagact cgtggtggtc atatggaagc tgaaaaggta aacgaagaaa ttgctaaaat
    21361 ccgaaatgtt gaaccttata aaacaatcaa ttcacctaac cgttacgaat ttattcataa
    21421 tgctgaagat ttgattcgtt tcgtcgatca gttgcagcaa ttaggtcaaa aaccagtagg
    21481 attcaaaatt gtagtaagca aagtttcaga aattgaaaca cttgtacgta cgatggtgga
    21541 actagataag tatccaagct ttattacgat tgatggtggt gaaggtggta ctggtgcaac
    21601 attccaagaa ttacaagatg gtgttggctt accgctattt acagctctac ctattgtgtc
    21661 tggcatgtta gaaaaatatg gtattcgaga taaagtgaaa ttggcggcat ctggtaagtt
    21721 agtgacacca gataaaattg cgattgcact aggtttaggt gcagattttg taaatatcgc
    21781 acgtgggatg atgattagtg tcggttgtat aatgagtcaa caatgtcaca tgaatacgtg
    21841 tcctgtaggt gttgcaacga cagatgcgaa gaaagaaaaa gcattgattg ttggagaaaa
    21901 gcaatatcgt gtcacaaact atgtaacaag tttgcatgaa ggcttattca atattgcagc
    21961 agctgttggc gtatccagtc ctacagaaat tactgctgat catattgtat atcgaaaagt
    22021 cgatggtgag ttacaaacga tacatgatta taaattaaaa ctcattagtt aacttaatta
    22081 tttcgggaaa ttgaaagcag cggattttag cgttactgca aataatttta tattagtagt
    22141 ggatgctggt cacacaagaa cttcaaatat taaagccctc agaatatgaa ttaaggtttg
    22201 taaccttagt cttatctgag ggcattttta agttataaac tatttgtcgt ccattttatc
    22261 tttttctttt aaacctctgt gctttaattg cttttcaagt ttttcaaaac taatatcttt
    22321 attttcttta gtcgaaacac caagacgttt atttaatttt ttcatgtcaa cttctgtgta
    22381 atctatgtct aagtgttcaa ttgctttttt atctttatag tctactttgt attttacgcc
    22441 tttaaggtct ttgaaaatac tttcagattt ggcgaataac tttttggctt cgtctttatc
    22501 catacctaga tcgtcatatt taattgtgtt gattgtagac tgttttaaaa ctttatcatc
    22561 tttatatgtg atagaagtta gtacatgttt accactaaca tcaccttcat atgttttggt
    22621 ttgttcttta ccacaagctg ataatgcaat gatacaaact aatgctacta caattaatga
    22681 acataatttt ttcaaagtca gtcgccttct ttcgatattt g
//
LOCUS       DALAGD010000046        22596 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.046, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000046 DALAGD010000000
VERSION     DALAGD010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22596
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.046"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(80..388)
                     /locus_tag="O2701_001400"
     CDS             complement(80..388)
                     /locus_tag="O2701_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HCZ4293300.1"
                     /translation="MTEHNHDSQLEINNEEELLTLFDEEGNEVLYRKVLEFYHPEFKK
                     EYVILAEEGAQSDEDDMIELVPMINEPDESGDGGKLVPIETDEEWDMIEEVVNTEMEE
                     "
     gene            complement(403..831)
                     /gene="ruvX"
                     /locus_tag="O2701_001401"
     CDS             complement(403..831)
                     /gene="ruvX"
                     /locus_tag="O2701_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCZ4293301.1"
                     /translation="MLQHKILGLDVGSRTVGIAISDIMGWTAQGLDTLRINEENNELG
                     IDQLVDIIKKHNVGTVVIGLPKNMNNSIGFRGEASLTYKEKLLEAYPSIEIVMWDERL
                     STMAAERSLLEADVSRQKRKQVIDKMAAVFILQGYLDSLH"
     gene            complement(835..1095)
                     /locus_tag="O2701_001402"
     CDS             complement(835..1095)
                     /locus_tag="O2701_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HCZ4293302.1"
                     /translation="MENFDKTMKFDYEELPTQDVRDVLNNVYRTLDERGYNAVNQIVG
                     YLLSGDPAYIPRQNEARNQIRHIDRDVIMEELVSYYLKEQNK"
     gene            complement(1158..3788)
                     /gene="alaS"
                     /locus_tag="O2701_001403"
     CDS             complement(1158..3788)
                     /gene="alaS"
                     /locus_tag="O2701_001403"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCZ4293303.1"
                     /translation="MKKLKASEIRQKYLDFFVEKGHMVEPSAPLVPIDDDTLLWINSG
                     VATLKKYFDGRETPKKPRIVNSQKAIRTNDIENVGFTARHHTFFEMLGNFSIGDYFKQ
                     EAIEFAWEFLTSDKWMGMEPDKLYVTIHPEDMEAYNIWHKDIGLEESRIIRIEGNFWD
                     IGEGPSGPNTEIFYDRGEAYGQDDPAEEMYPGGENERYLEVWNLVFSEFNHNKDHSYT
                     PLPNKNIDTGMGLERMASVSQNVRTNYETDLFMPIMNEIEKVSGKQYLVNNEQDVAFK
                     VIADHIRTIAFAISDGALPANEGRGYVLRRLLRRAVRFSQTLGINEPFMYKLVDIVAD
                     IMEPYYPNVKEKADFIKRVIKSEEERFHETLEDGLAILNELIKKAKATTNEINGKDAF
                     KLYDTYGFPIELTEEIAVQAGLKVDMTTFESEMQQQRDRARQARQNSQSMQVQSEVLK
                     NITSASTFVGYDTATAQTTLTHLIYNGEEVSQVEAGETVYFMLTETPFYAISGGQVAD
                     TGIVYNDNFEIAVSEVTKAPNGQNLHKGVVQFGQVNVGATVSAEVNQNDRRDIQKNHS
                     ATHLLHAALKSVLGDHVNQAGSLVEADRLRFDFSHFGPMTNDEIDQVERLVNEEIWKG
                     IDVNIQEMDIASAKEMGAMALFGEKYGDVVRVVNMAPFSIELCGGIHVRNTSEIGLFK
                     IVSESGTGAGVRRIEALTGKAAFLYLEDIQEKFNTMKSQLKVKSDDQVVDKLTQLQDE
                     EKALLKQLEQRDKEITSLKMGNIEDQVEEINGYKVLVTEVDVPNAKAIRSTMDDFKSK
                     LQDTIIILASNVDDKVSMVATVPKSLTNNVKAGDLIKQMAPIVGGKGGGRPDMAQGGG
                     TQPENISKSLSFIKDYIKNL"
     gene            complement(4131..6608)
                     /locus_tag="O2701_001404"
     CDS             complement(4131..6608)
                     /locus_tag="O2701_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HCZ4293304.1"
                     /translation="MSDPTLFDYSMIKGTVEAILFQNSDNFYTVLKVDTIETNEDFDT
                     MPTVVGFLPNIVEGDVYTFKGQVVDHPRYGKQLKAETFEKEMPQTKEAIISYLSSDLF
                     KGVGKKTAQNIVNTLGDNAINDILDDHSVLEKVSGLSKKKQKQIAEQISANQESEKIM
                     IRLHDLGFGPKLSMAIYQFYLGDTLTILDRNPYQLIYDIKGIGFNKADQLARNIGIAY
                     NDNERLKAALLYTLEEECIKQGHTYLPINVVIDLTVDVLNYQDEEVIEPEKLDEMLQY
                     LNEEKRLIIDNEQVAIPSLYYSEIKSVQNLFRIKTHTNKLTEIEQSDLQMHIGEIEDA
                     NQVNYAASQKEALQTAINSKVMLLTGGPGTGKTTVIKGIVELYAEIHGLSLDYDDYVN
                     DDYPVVLAAPTGRASKRLQESTGLEAMTIHRLIGWNQDTKPEDILENEINARLIIIDE
                     MSMVDTWLFHQFLSAVPLDAQLIFVGDEDQLPSVGPGQVFKDLIESKAIPRVNLTEVY
                     RQQDGSSIIELAHRMKLGQKIDITQRFHDRSFINCQANQIPTVVEKVVTSAVNKGYTM
                     ADIQVLAPMYKGNAGIKRLNQVLQDILNPKKKDTREIEFGDVVFRKGDKVLQLVNRPN
                     DNIFNGDIGVIVGIFWAKENALNKDVLVVDFEGNEITFTKQDMMELTHAYCTSIHKSQ
                     GSEFPIVIMPIVKQYFRMLQRPILYTGLTRAKTSLVLLGDPEAFDIGLKTNGQARLTQ
                     LCTLLKNYFNSEDEEMLENTATNDTGASQTTIDDQVEAPMSSNDSEEVMSDSTKTDTN
                     VLTEATMFKIDPMINMGEITPYDFIER"
     gene            complement(6610..7278)
                     /locus_tag="O2701_001405"
     CDS             complement(6610..7278)
                     /locus_tag="O2701_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCZ4293305.1"
                     /translation="MIDQQTIYQYIQNGKIEEALQALFGNIEENPTIIENYINAGIVL
                     ADANEIEKAERFFQKALTIDPKNGVVFFNLANVYYNQQRYQEAIKLYQQALQTEIEQV
                     DCNYMIGMAFNQLESFKLALPYLMTAAELDKDKDAEVQFQYGLVLCQLEMFNEAITQL
                     KHVLTIDKNHVDARYNLGLALFMKNEDIDEAITHFKEAVTIDPKHLLSQHALKTFTKM
                     KEEE"
     gene            complement(7740..8858)
                     /gene="mnmA"
                     /locus_tag="O2701_001406"
     CDS             complement(7740..8858)
                     /gene="mnmA"
                     /locus_tag="O2701_001406"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCZ4293306.1"
                     /translation="MSNKDIRVVVGMSGGVDSSVTAHVLKEQGYDVIGIFMKNWDDTD
                     ENGVCTATEDYNDVIEVCNQIGIPYYAVNFEKEYWDKVFTYFLDEYKKGRTPNPDVMC
                     NKEIKFKAFLDHAMNLGADYVATGHYARIHRHEDGHVEMLRGVDNNKDQTYFLNQLSQ
                     QQLSKVMFPIGDIEKSEVRRIAEEQGLVTAKKKDSTGICFIGEKNFKTFLSQYLPAQP
                     GDMITLDGKKMGKHSGLMYYTIGQRHGLGIGGDGDPWFVVGKNLKDNVLYVEQGFHHD
                     ALYSDYLIASDYSFVNPEDNDLDQGFECTAKFRYRQKDTKVFVKRENDHALRVTFAEP
                     VRAITPGQAVVFYQGDVCLGGATIDDVFKNEGQLNYVV"
     gene            complement(8859..10001)
                     /locus_tag="O2701_001407"
     CDS             complement(8859..10001)
                     /locus_tag="O2701_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCZ4293307.1"
                     /translation="MEIYADYAATTPVKPEVVDAMMTIYNSHYGNPSSIHAKGRDARK
                     YLDESRRQIAQLLGADTHEIIFTSGATESNNTAIKGIVKANEQLGNHIITSKIEHHSV
                     LHVFEQLEREGFDVTYLDVDDTGAIDLDQLEETITDKTILVSIMFVNNEVGTVQQIYD
                     IQDIIAETNAYFHVDAVQAIGHLDVKFDEFEIDAMSITAHKFGGPKGVGALLVKDHVT
                     LDYPQLGGEQELKRRAGTENLAQIVGMAKALQLAEKNRDDNNIHLMNLKEQFLVKLQE
                     RAIPFELNGSMTDATGHIVNLYFPFVEVETMLTLLDMAQIYVSSGSACTAGSTQPSHV
                     LDAMFEDEERSNHSIRFSFNELTTENEINAIVAEIHKIYFKFKEES"
     gene            10313..11326
                     /locus_tag="O2701_001408"
     CDS             10313..11326
                     /locus_tag="O2701_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCZ4293308.1"
                     /translation="MAIKYSALNLVPIREGEDEQTAINDMVNLAQHLDELSYERYWIA
                     EHHNAPNLVSSATALLIQHTLEHTKHIRVGSGGIMLPNHAPLIVAEQFGTMATLFPNR
                     VDLGLGRAPGTDMMTASALRRDQHDGVYKFPEEVSLLQQYFGPAHQQAYVRAYPAVGK
                     NVPLYILGSSTDSAHLAARKGLPYVFAGHFAPQQMKEAIEIYKTLFEPSDVLDEPYVI
                     VCLNTIVAENDDEAQYLASSMAQVMVSITRGRMQPVQPPTHELQNILTPREYAMAMER
                     QKISLIGSENTVQQKIQDFMETYGEVNEIMAISYIYDKDMQLDSYRRFKNVINQINEK
                     NTL"
     gene            complement(11563..11709)
                     /locus_tag="O2701_001409"
     CDS             complement(11563..11709)
                     /locus_tag="O2701_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293309.1"
                     /translation="MSFMDKAKDAVEKFKNSDNEQVKNVKDKINEYTGSNNEEKKENE
                     DKEK"
     gene            complement(11749..11931)
                     /locus_tag="O2701_001410"
     CDS             complement(11749..11931)
                     /locus_tag="O2701_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCZ4293310.1"
                     /translation="MADESKFDQFKGNVKETVGNVTDNKELEKEGQQDKVIGKAKEVV
                     ENAKNKITDAIDKLKK"
     gene            complement(12031..12453)
                     /locus_tag="O2701_001411"
     CDS             complement(12031..12453)
                     /locus_tag="O2701_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCZ4293311.1"
                     /translation="MKISTKGRYGLTLMISLAKKEGQGCISLKSIAEENNLSDLYLEQ
                     LVGPLRNAGLIRSVRGAKGGYQLRVPAEEISAGDIIRLLEGPITFVESIESEPPAQKQ
                     LWIRMRDAVRDVLDNTTLKYLAEYVDTSEDLDGYMFYI"
     gene            12538..13812
                     /locus_tag="O2701_001412"
     CDS             12538..13812
                     /locus_tag="O2701_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HCZ4293312.1"
                     /translation="MSTEPLASRMRPKNIDEIISQQHLVGPRGIIRRMVDTKKLTSMI
                     FYGPPGIGKTSIAKAISGSTQYKFRQLNAVTNTKKDMQLVVEEAKMSGQVILLLDEIH
                     RLDKAKQDFLLPHLENGKIVLIGATTSNPYHAINPAIRSRAQIFELYPLNDEDVRQAL
                     TRAIEDDENGLKTYQPKIDEDAMTYFSTQSQGDVRSALNALELAVLSADNDKDGYRHV
                     TLQDAKDCLQKGAFVSDKDGDMHYDVMSAFQKSIRGSDVNAALHYLARLIEAGDLPTI
                     VRRLLVISYEDIGLASPNAGQRTLAAIESAERLGLPEARIPLSQAVIELCLSPKSNSA
                     MSAIDSALSDIRNGHVGQIPNHLKDGHYQGAKDLGRSIGYKYPHQYVNGYVSQQYLPD
                     KLKNKIYYEPKTTSKSEQQLKEIYNNLLKQRP"
     gene            complement(13973..14746)
                     /locus_tag="O2701_001413"
     CDS             complement(13973..14746)
                     /locus_tag="O2701_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HCZ4293313.1"
                     /translation="MKHQFSRNELAIGQQGLDLLKQQTVVILGVGGVGSFAAEALART
                     NIGHIILIDKDDVDITNVNRQLHALTTTVGQSKVSLMEERIKLINPDCKVTPLHMFYT
                     EETYEEIFDNYDIDYFVDASDTIMYKVHLMKECLDRGIKVISSMGAANKTDPTRFTIS
                     DISKTHTDPMAKIIRQKLKKLGIRKGIPVVFSDESPIVIREDVKDIVGDKNAINRKGQ
                     MPPSSNAFVPSVVGLICASYVVNDVLKDIPVRRIKDKGQ"
     gene            complement(14746..14874)
                     /locus_tag="O2701_001414"
     CDS             complement(14746..14874)
                     /locus_tag="O2701_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293314.1"
                     /translation="MGFIAFNKCFLVEFWENNQSDNAKYKILSYYVLMYKSVKEVK"
     gene            complement(14939..15129)
                     /gene="ssrS"
                     /locus_tag="O2701_001415"
     ncRNA           complement(14939..15129)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="O2701_001415"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(15207..16973)
                     /gene="aspS"
                     /locus_tag="O2701_001416"
     CDS             complement(15207..16973)
                     /gene="aspS"
                     /locus_tag="O2701_001416"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCZ4293315.1"
                     /translation="MSKRTTYCGLVTEAFLGQEITLKGWVNNRRDLGGLIFVDLRDRE
                     GIVQVVFNPAFSEEALKIAETVRSEYVVEVQGTVTKRDPETVNPKIKTGQVEVQVTNI
                     KVINKSETPPFSINEENVNVDENIRLKYRYLDLRRQELAQTFKMRHQITRSIRQYLDD
                     EGFFDIETPVLTKSTPEGARDYLVPSRVHDGEFYALPQSPQLFKQLLMISGFDKYYQI
                     VKCFRDEDLRADRQPEFTQVDIEMSFVDQEDVMQMGEEMLKKVVKEVKGVEINGAFPR
                     MTYKEAMRRYGSDKPDTRFEMELIDVSQLGRDMDFKVFKDTVENDGEIKAIVAKGAAE
                     QYTRKDMDALTEFVNIYGAKGLAWVKVVEDGLTGPIGRFFETENVETLLTLTGAEAGD
                     LVMFVADKPNVVAQSLGALRVKLAKELGLIDETKLNFLWVTDWPLLEYDEDAKRYVAA
                     HHPFTSPKEADIAKLGTAPEEAEANAYDIVLNGYELGGGSIRIHDGELQEKMFEVLGF
                     TKEQAQEQFGFLLDAFKYGAPPHGGIALGLDRLVMLLTNRTNLRDTIAFPKTASATCL
                     LTNAPGEVSDKQLEELSLRIRH"
     gene            complement(16989..18251)
                     /locus_tag="O2701_001417"
     CDS             complement(16989..18251)
                     /locus_tag="O2701_001417"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCZ4293316.1"
                     /translation="MIKIPRGTQDILPEDSKKWRYIENQLDELMTFYNYKEIRTPIFE
                     STDLFARGVGDSTDVVQKEMYTFKDKGDRSITLRPEGTAAVVRSYIEHKMQGNPNQPI
                     KLYYNGPMFRYERKQKGRYRQFNQFGVEAIGAENPSVDAEVLAMVMHIYQSFGLKHLK
                     LVINSVGDMASRKEYNEALVKHFEPVIHEFCSDCQSRLHTNPMRILDCKVDRDKEAIK
                     TAPRITDFLNEESKAYYEQVKAYLDDLGIPYIEDPNLVRGLDYYTHTAFELMMDNPNY
                     DGAITTLCGGGRYNGLLELLDGPSETGIGFALSIERLLLALEEEGIELDIEENLDLFI
                     VTMGDQADRYAVKLLNHLRHNGIKADKDYLQRKIKGQMKQADRLGAKFTIVIGDQELE
                     NNKIDVKNMTTGESETIELDALVEYFKK"
     gene            complement(18712..19587)
                     /locus_tag="O2701_001418"
     CDS             complement(18712..19587)
                     /locus_tag="O2701_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCZ4293317.1"
                     /translation="MKKIEAWLSKKGLKNKRTLIVVIAFVLFIIFLFLLLNSNSEDSG
                     NITITENAELRTGPNAAYPVIYKVEKGDHFKKIGKVGKWIEVEDTSSNEKGWIAGWHT
                     NLDIVADNTKEKNPLQGKTIVLDPGHGGSDQGASSNTKYKSLEKDYTLKTAKELQRTL
                     EKEGATVKMTRTDDTYVSLENRDIKGDAYLSIHNDALESSNANGMTVYWYHDNQRALA
                     DTLDATIQKKGLLSNRGSRQENYQVLRQTKVPAVLLELGYISNPTDETMIKDQLHRQI
                     LEQAIVDGLKIYFSA"
     gene            complement(19584..20036)
                     /locus_tag="O2701_001419"
     CDS             complement(19584..20036)
                     /locus_tag="O2701_001419"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCZ4293318.1"
                     /translation="MKVVVQRVKEASVTNDTLNNQIKKGYCLLVGIGQNSTEQDADVI
                     AKKIANARLFEDDNNKLNFNIQQMNGEILSVSQFTLYADVKKGNRPGFSNSKNPDQAV
                     KIYEYFNDALRAYGLTVKTGEFGTHMNVSINNDGPVTIIYESQDGKIQ"
     gene            complement(20048..22237)
                     /locus_tag="O2701_001420"
     CDS             complement(20048..22237)
                     /locus_tag="O2701_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HCZ4293319.1"
                     /translation="MNNEYPYSADEVLHKAKSYLSADEYEYVLKSYHIAYEAHKGQFR
                     KNGLPYIMHPIQVAGILTEMRLDGPTIVAGFLHDVIEDTPYTFEDVKEMFNEEVARIV
                     DGVTKLKKVKYRSKEEQQAENHRKLFIAIAKDVRVILVKLADRLHNMRTLKAMPREKQ
                     IRISRETLEIYAPLAHRLGINTIKWELEDTALRYIDNVQYFRIVNLMKKKRSEREAYI
                     ETAIDRIRTEMDRMNIEGDINGRPKHIYSIYRKMMKQKKQFDQIFDLLAIRVIVNSIN
                     DCYAILGLVHTLWKPMPGRFKDYIAMPKQNLYQSLHTTVVGPNGDPLEIQIRTFDMHE
                     IAEHGVAAHWAYKEGKKVSEKDQTYQNKLNWLKELAEADHTSSDAQEFMETLKYDLQS
                     DKVYAFTPASDVIELPYGAVPIDFAYAIHSEVGNKMIGAKVNGKIVPIDYILQTGDIV
                     EIRTSKHSYGPSRDWLKIVKSSSAKGKIKSFFKKQDRSSNIEKGRMMVEAEIKEQGFR
                     VEDILTEKNIQVVNEKYNFANEDDLFAAVGFGGVTSLQIVNKLTERQRILDKQRALNE
                     AQEVTKSLPIKDNIITDSGVYVEGLENVLIKLSKCCNPIPGDDIVGYITKGHGIKVHR
                     TDCPNIKNETERLINVEWVKSKDATQKYQVDLEVTAYDRNGLLNEVLQAVSSTAGNLI
                     KVSGRSDIDKNAIINISVMVKNVNDVYRVVEKIKQLGDVYTVTRVWN"
BASE COUNT         6903 a         4402 c         3145 g         8146 t
ORIGIN      
        1 aattatttat acattgcttg acttaaaaaa tactcttgcc tccccatctt taaggttagc
       61 aagagtaaaa tctttttaat tattcttcca tttcagtatt tacaacttct tcaatcatgt
      121 cccattcttc atcagtttcg attggtacta acttaccacc gtcacctgac tcatctggtt
      181 cattgatcat tggtacaagc tcaatcatat cgtcttcatc tgattgagca ccttcttcag
      241 ctaagataac atactctttt ttgaattcag gatgataaaa ttctaaaact tttcggtata
      301 aaacttcatt tccctcttca tcgaataaag ttaataattc ttcttcgtta ttaatttcta
      361 gttgtgaatc atgattatgt tcagtcatag taaaatctcc ttttaatgta gtgaatctaa
      421 atagccttgt aaaataaata ccgctgccat tttatcaatc acttgttttc ttttttgtct
      481 tgaaacatct gcttctaata atgatcgttc agcagccatt gtgcttaatc tttcatccca
      541 catcacaatc tcaatagaag gataagcttc taataatttt tctttatatg ttaacgaagc
      601 ttcgcctcga aatcctattg aattattcat gtttttaggt agtcctatta cgactgtacc
      661 cacattatgt tttttaataa tgtctactaa ttggtcaata cctaattcat tattttcttc
      721 attgattcgg agtgtgtcta atccttgtgc cgtccaaccc attatatcac taattgcaat
      781 tcctaccgtt ctactaccga catcgagtcc taaaatttta tgttgtaaca taaattattt
      841 attttgctct tttaaatagt aagaaacaag ctcttccata ataacatctc tatcaatatg
      901 acgaatttga tttcttgctt cattttggcg tggaatatac gcagggtcac ctgataataa
      961 ataacctaca atttggttta cggcattata tcctcgttca tctaatgttc gataaacatt
     1021 atttaaaaca tctcttacat cttgcgttgg aagttcttca tagtcgaatt tcattgtttt
     1081 atcaaagttt tccatttgcg acactccttt aattacaaat ataactcact atcatcatac
     1141 aatattatgg ctttaaatta tagattttta atgtaatctt taataaagct taatgatttt
     1201 gagatatttt caggttgtgt accgccacct tgagccatat ctggacgacc gccaccttta
     1261 ccaccaacga ttggtgccat ttgtttgata agatcaccgg ctttaacgtt atttgttaaa
     1321 gatttaggga cagttgcaac catcgatact ttatcatcaa cattacttgc aagaatgata
     1381 attgtatctt gtagtttaga tttaaaatcg tccattgtcg agcgaattgc tttcgcattt
     1441 ggtacatcca cttcagtaac caatacttta tagccattga tttcttcaac ttgatcttca
     1501 atattaccca ttttaagtga tgtgatttct ttgtcacgtt gctctaattg ttttaataat
     1561 gctttttctt catcttgtaa ttgtgttaac ttatcgacta cttgatcatc agatttcact
     1621 ttcagctgtg atttcatcgt attaaatttc tcttgaatat cttctaaata taagaaagct
     1681 gctttacctg ttaatgcttc aatacgacgc acaccagctc ctgtacctga ctcacttact
     1741 attttgaata agccaatttc agaagtattg cggacatgaa taccaccaca taattcaatt
     1801 gaaaatggtg ccatatttac tacacgcaca acatcaccat atttttcacc gaataatgcc
     1861 attgcgccca tttctttagc tgaagcaata tccatttctt gaatgttaac gtcaatacct
     1921 ttccaaattt cttcatttac taagcgttca acttgatcaa tttcatcatt agtcattgga
     1981 ccaaaatgag agaaatcaaa acgtaaacga tctgcttcta ctagtgaacc agcttggtta
     2041 acatgatcac ccagtactga tttcaacgct gcatgtaata aatgtgttgc actatggttc
     2101 ttttgaatgt cacgtcgatc attttggttc acttcagcag acactgtagc gccaacattt
     2161 acttggccaa attgtactac tcctttatgc aagttttgac catttggtgc tttggttact
     2221 tcactaacag caatttcaaa attgtcatta taaacaatac ctgtatccgc aacttgtcca
     2281 ccactgattg cataaaatgg tgtttccgtt aacatgaagt atactgtttc acccgcttca
     2341 acttgtgaaa cttcttcacc attgtatatc aagtgtgtta gtgttgtttg agctgtcgca
     2401 gtatcataac caacaaaagt acttgcagat gtaatatttt tcaatacttc actttgaact
     2461 tgcattgatt gagaattttg acgtgcttga cgtgcacgat cacgttgttg ttgcatttct
     2521 gactcgaatg ttgtcatatc aactttcaat cctgcttgca ctgctatttc ttcagttaat
     2581 tcaattggga acccatacgt atcatacaat ttaaatgcat ctttcccatt aatttcattt
     2641 gttgtcgctt tagctttttt aattaattca tttaaaatcg ctaaaccatc ttctaatgtt
     2701 tcatggaatc gttcttcttc agactttata acacgcttaa tgaaatctgc tttttcctta
     2761 acatttggat aatatggttc cataatgtct gcaacaatat caacaagttt gtacataaat
     2821 ggctcattga ttcctaacgt ttgactaaaa cgaacggcac gacgtaacaa tcgacgtaat
     2881 acataccctc taccttcatt ggcaggtaat gcaccatcag aaattgcaaa tgcaatcgta
     2941 cgaatgtggt cagcaattac tttaaatgcc acatcttgtt cgttgtttac taaatattgt
     3001 ttacctgata ctttttcgat ttcattcatt ataggcataa ataaatctgt ttcatagtta
     3061 gtacgtacat tttgagaaac tgaggccata cgctcaagcc ccatgccagt atcaatattt
     3121 ttattaggta atggtgtgta actatgatct ttattatgat tgaattcact aaatactaag
     3181 ttccatactt caagatagcg ttcattttct ccacctggat acatttcttc tgccggatcg
     3241 tcttgtccat atgcttctcc gcgatcatag aaaatctcag tgttcggtcc tgaaggccct
     3301 tcaccaatat cccagaagtt accttcaatg cgaataatac gactttcttc aagcccaata
     3361 tctttatgcc aaatgttgta tgcttccata tcttccggat gaatcgtaac gtacaattta
     3421 tctggctcca tacccatcca tttatcactc gttaaaaatt cccaagcaaa ttcaatcgct
     3481 tcttgtttaa aataatcacc aattgagaag ttacctaaca tttcaaagaa tgtatggtga
     3541 cgcgctgtga aaccaacatt ttcaatatca tttgtacgaa tagctttttg agagtttaca
     3601 attcttggct ttttaggtgt ttcacgtcca tcaaaatatt tctttaatgt tgctacacct
     3661 gaattaatcc ataataatgt atcatcatca attggcacta atggtgcaga aggttcaacc
     3721 atatgtcctt tttcaacaaa gaaatctaga tatttttgtc taatttcact cgcttttaac
     3781 tttttcatca tttacacatc ctatttactg tttttaaatt accattccat aaaaattgat
     3841 gacacagata gtcgatttgc aaaactagta taaatcaata tcatttttta ttattaaaaa
     3901 ataaaaaacg cccatcctca aaagggacga acgttatcgc ggtaccaccc tagttataaa
     3961 tgcaattcaa cacatttatc actttaattc gactatacag ttgtgcataa agtagcgttc
     4021 actaatgttt gttgtacttt tcaccaacca gtacatctct gataaacaaa tcattaacta
     4081 ctcatcttta tacgaattta attctatttt agttacattt acgcttgttg tcaacgttct
     4141 ataaagtcat acggcgtgat ttctcccata ttaatcattg ggtcaatttt aaacattgta
     4201 gcttccgtta atacatttgt atctgttttt gttgaatcag acataacttc ttcactatca
     4261 ttcgatgaca ttggcgcttc tacttgatca tctattgtcg tttgtgaagc tcctgtatca
     4321 ttagttgctg tgttttccag catttcttca tcttctgaat taaaataatt tttcaacaat
     4381 gtacataatt gtgttaaacg cgcttgacca tttgttttca atccaatatc aaatgcttcc
     4441 ggatcaccaa gtaaaactaa actcgttttc gctctagtta aaccagtata taatatcggt
     4501 ctttgtaaca ttctaaaata ctgtttaaca ataggcatga taacaatagg aaattctgaa
     4561 ccttgtgatt tatggattga tgtacaataa gcatgtgtta attccatcat atcttgtttc
     4621 gtaaatgtaa tttcattacc ttcaaaatcc acaacaagta catctttatt aagggcattt
     4681 tctttcgccc aaaaaatacc aacaataact cctatgtcac cattgaatat gttatcattt
     4741 ggcctattaa caagttgtaa tactttgtca ccttttctaa agactacatc accaaactca
     4801 atttctcgtg tgtctttctt tttagggttt aaaatatctt gtaaaacttg atttaaacgt
     4861 ttaataccgg catttccttt atacattggt gcaagcactt gaatatcagc catagtatac
     4921 cctttattaa cagcactagt aactaccttc tcaacaactg ttggtatttg gtttgcctga
     4981 cagttaataa aacttctatc atgaaaacgc tgtgtaatat caattttctg acccaacttc
     5041 attcgatgtg ctaattctat aatgcttgaa ccatcttgtt gacgatatac ttcagtcaga
     5101 tttactcgtg gtatagcttt cgattcaatt aaatctttaa atacttgacc aggacctaca
     5161 gaaggcaatt ggtcctcatc acctacaaat atcaattgtg catctaaagg aactgcactt
     5221 aaaaattggt ggaacaacca agtatctacc atagacatct catcaatgat tatgagtcgt
     5281 gcgtttattt cattttctaa tatatcctct ggctttgtgt cttgattcca acctattaaa
     5341 cgatgaatcg tcattgcttc taatccagtt gactcttgta gtctcttaga cgctcttcct
     5401 gttggcgctg ctaatacaac tggataatca tcattgacat aatcatcata atctaatgat
     5461 aagccatgaa tctcagcata taattcaaca atacctttaa ttactgtcgt ttttcctgtt
     5521 cccggtccac cggttaatag catcacctta gaattgatag ccgtttgcaa agcttctttt
     5581 tgtgaagctg catagttcac ttgattcgca tcttctattt caccaatatg catttgtaaa
     5641 tctgactgtt caatttctgt aagtttattt gtatgcgtct ttattctgaa taagttttga
     5701 acacttttga tttcagaata atacaaactt ggaattgcaa cttgttcatt gtcaataatt
     5761 agtcgttttt cctcatttaa gtattgcaac atttcgtcta atttttcagg ttcgatgacc
     5821 tcttcatctt gataatttaa tacatcaacc gttaaatcta taacaacatt gataggcaaa
     5881 tatgtatgtc cctgtttaat acattcttct tctaacgtat agagcaacgc agcttttaat
     5941 cgttcattat cgttataagc gataccaata tttctagcaa gttgatctgc tttattaaaa
     6001 ccaatacctt taatatcata aatcaattga tatggatttc gatctaaaat agtcagtgta
     6061 tcgccgagat aaaactgata aattgccatt gaaagtttag gaccaaaccc taaatcatgt
     6121 aaacgaatca ttattttttc agattcttga tttgctgaaa tttgttctgc aatttgtttc
     6181 tgtttctttt tagataatcc cgaaactttt tctagcactg aatggtcatc taatatatca
     6241 tttatcgcat tgtcacctaa tgtattaaca atattttgag ctgtcttttt acctacacct
     6301 ttaaacaaat cactagataa ataacttata attgcttctt tcgtttgtgg catttctttt
     6361 tcaaaagtct ctgcttttaa ttgtttacca taacgtggat gatcaacaac ttgcccttta
     6421 aatgtgtaga catcgccttc aacaatattc ggaagaaacc ctacaacagt tggcattgta
     6481 tcaaagtctt catttgtttc aatagtatct actttaagca ctgtataaaa attatcactg
     6541 ttttgaaaca atatcgcttc aacagtacct ttgatcattg aataatcaaa tagtgtaggg
     6601 tctgacatgt tactcctcct ctttcatttt agtgaatgtt ttcagcgcat gctgacttaa
     6661 taagtgttta gggtcgatag tcacagcttc tttaaaatga gttattgctt catcaatatc
     6721 ttcatttttc ataaataacg ctaagcccaa attgtatctt gcatcaacat gatttttatc
     6781 aatcgttaat acatgtttaa gttgagttat ggcttcatta aacatttcta attgacataa
     6841 tacaagacca tattgaaatt gaacttctgc atctttgtct ttatctagtt ccgcagcagt
     6901 cattaaatac ggcaatgcca gcttaaatga ttctaactga ttaaacgcca taccgatcat
     6961 ataattacaa tcaacttgtt caatctctgt ttgtaatgct tgttgatata atttaatagc
     7021 ttcttgataa cgttgctgat tataatatac atttgctaga ttaaaaaata cgacgccatt
     7081 cttcggatct attgttaaag ctttttggaa aaaacgctct gccttttcaa tctcattcgc
     7141 atcagcaagt acgataccag cattaatata attttcaata attgtaggat tttcttcgat
     7201 atttccgaac aatgcttgta acgcttcttc tatttttcca ttttgtatgt attgataaat
     7261 tgtttgttga tctatcattt acgaacctca tttctcatca attataacat cttgataaat
     7321 tgtatgtctc gaatcactta acaacgaata aaatataatc taatatcatc ttcattcatg
     7381 aaaaagcggg aatggaatag aaatgcttaa gaaccattaa cggtttatta tgtaatggtt
     7441 cttccacatt agccaccact attatgtact taaaaataag aatacataat tagattcatg
     7501 catagggagt gggacagaaa tgatatttta acaaaattaa attcgttatc cccaactggc
     7561 attgcctgta gaatttcttt acgaaattct ctatgttgtg gtcccgccaa tataacattg
     7621 tagagcctag gacattgtga tgtcccagac tctatcctca tgaattattc tcatcaaaaa
     7681 ctgtctttcg tcattttcaa cgttgaaact tcaaataagt aatttattgt tgccattgtt
     7741 tatacaacat aatttaattg accttcattt ttgaacacat cgtcaattgt tgcaccacca
     7801 agacacacat caccttgata aaaaacaact gcttgtccag gtgtgattgc tcttactggc
     7861 tcagcaaaag taacacgtag tgcatggtcg ttttcacgtt tcacaaaaac tttcgtatct
     7921 ttttggcgat atctaaattt agctgtacat tcaaaacctt gatctaagtc attatcttct
     7981 ggatttacaa atgaatagtc tgaagcaatt aagtaatcac tgtataatgc atcgtgatgg
     8041 aatccttgtt ctacatataa aacattatct tttaggtttt taccgacaac aaaccaagga
     8101 tcgccatctc cacctatacc taatccatgt ctttgtccta ttgtgtaata catcaaacca
     8161 ctatgtttac ccattttctt accatcaagt gttatcatat cacccggttg tgcaggtaaa
     8221 tattgtgata aaaatgtttt aaagtttttt tcgccgataa aacaaatgcc tgtagaatct
     8281 tttttcttag cagtaacaag tccttgttct tcagcaattc gacgcacttc actcttttcg
     8341 atgtcgccaa ttgggaacat cacttttgaa agttgttgtt gagataattg attcaagaag
     8401 tatgtttgat ctttattatt atctacacca cgtaacattt caacatgacc atcttcatga
     8461 cgatgtatgc gtgcgtaatg tcctgttgct acataatctg cacctaaatt catcgcatga
     8521 tctaaaaagg ctttaaactt aatttcttta ttacacataa cgtctggatt tggagtacga
     8581 ccttttttgt attcatctaa gaaatacgta aagactttat cccaatattc tttttcaaaa
     8641 ttaacagcgt aatacggaat gccaatttga ttacacactt caataacatc gttgtaatct
     8701 tcagttgcag tacatacgcc attttcgtca gtgtcatccc agtttttcat aaatatgcca
     8761 atgacatcat aaccttgttc ttttaagacg tgggctgtta cagaactatc tacaccgcct
     8821 gacataccaa cgacaacacg tatatcttta tttgacaatt atgactcctc cttaaattta
     8881 aaatatattt tatgaatttc agctacaatt gcattaattt cattttcagt agtcaattcg
     8941 ttaaaactaa atcgaatcga atgatttgat cgctcctcat cttcgaacat tgcatctaaa
     9001 acatgcgacg gttgtgtaga gcctgctgta catgcagatc cagacgacac atagatttgt
     9061 gccatatcca acaatgttaa catcgtttca acttcaacaa acggaaaata tagatttaca
     9121 atatggcctg tagcatccgt cattgaacca tttaattcaa atggaatcgc tctttcttgt
     9181 aatttaacta aaaattgttc ttttaaattc attaaatgaa tattgttatc gtctcgattc
     9241 ttttctgcta attgtaatgc tttagccatc ccaacaattt gcgcaagatt ttcagtgcct
     9301 gcacggcgtt tcaattcttg ttcaccgcca agttgaggat aatctagtgt aacatggtct
     9361 ttaactagta atgcaccgac accttttggt ccgccaaact tatgagcagt aatactcatt
     9421 gcgtcgatct caaattcgtc aaacttaaca tcaagatgtc caattgcttg aaccgcatca
     9481 acatggaaat atgcatttgt ctcagcaata atatcttgaa tatcataaat ttgttgcact
     9541 gtgccaactt cattatttac aaacataata gatactaaaa tcgtcttatc tgtaattgtt
     9601 tcttcaagtt gatctaaatc aatagcacct gtatcatcaa catctagata tgttacatca
     9661 aaaccttctc gctctaattg ttcaaaaaca tgtaacacag aatgatgttc aatcttcgat
     9721 gtgataatgt gattacccaa ttgttcattt gcttttacta tgcctttaat tgccgtatta
     9781 ttcgattctg ttgcgccact cgtaaatata atttcatgtg tatctgcacc aagtaattgt
     9841 gcaatttgac gtcttgactc atctaaatat ttacgcgcat ctcttccctt agcatgtatt
     9901 gatgatggat taccataatg cgaattgtaa atcgtcatca tcgcatctac tacttcaggt
     9961 tttactggtg tggtcgcagc ataatctgca taaatttcca tgtttggaca ctcctcacaa
    10021 ttttatcaat gttccaataa tagcacctta catactattt ttctactttt ctgtttaact
    10081 ttatttataa tgtttttaat tatattttac cattttctac acatgctttt cgataggctt
    10141 ttttaagttt atcgctttat tcttgtcttt tttataaatt ttagtatttg cagatatttt
    10201 tttatttgta aaatgtaacg tactattatt ttggttatga gcaatttaat atttatctgg
    10261 ttattcgatt ggtatacttc ttatatcata aaaaaggaag gacgatataa aaatggcgat
    10321 taaatattca gcattaaacc ttgtccctat tcgagaaggt gaagatgaac aaacagcaat
    10381 taatgatatg gttaatctcg cacaacattt agacgaatta tcatatgaaa gatattggat
    10441 tgctgaacac cataacgctc ccaacctagt aagttcagca actgctttat taattcaaca
    10501 tacgttagaa catacgaaac acatacgtgt aggttctgga ggcatcatgt tacctaatca
    10561 tgctccatta atcgttgcgg aacaatttgg cacgatggca acattatttc caaatcgtgt
    10621 cgatttagga ttaggacgtg cacctggaac agatatgatg accgcaagtg cattaagacg
    10681 agatcaacat gatggtgttt ataaatttcc agaagaggtt tcattattac aacaatattt
    10741 cggccctgct caccaacaag catatgttcg tgcttatcca gcagtaggta aaaatgtgcc
    10801 tttatacatt cttggttctt caacagattc tgcacattta gctgctcgca aagggcttcc
    10861 atatgtgttc gctggacatt ttgcacctca acaaatgaaa gaagctatcg aaatttacaa
    10921 aacgttattt gaaccttctg atgtattaga cgaaccttat gttattgtat gtttaaatac
    10981 aatcgttgct gaaaatgatg acgaagcaca atatttagct tcatctatgg cacaagtaat
    11041 ggttagtatc actcgtggca gaatgcagcc cgttcaaccg ccaacacatg aactacaaaa
    11101 tatattaacg ccgagagaat acgcgatggc tatggaaaga cagaaaatat cattaatagg
    11161 ttcagaaaat actgttcaac aaaaaattca agattttatg gaaacttatg gtgaagtcaa
    11221 cgaaattatg gcaataagtt atatttatga taaagatatg caattagact cttatcgtcg
    11281 gttcaagaat gttataaatc agataaatga aaaaaacact ttataatgtg ataaataaac
    11341 taagtgaaag tatgtatcca taatattaat aaaaatatac agtaacagca ttttgaatga
    11401 aagatgtctt tattgttcaa tcatttattt tagtaatgat tcaaattcac ttaaaattct
    11461 aatgcaaata tgaaagcgcc ccttcaactt tacactgtgt aagtgtttat ttgatggggc
    11521 gctttcaaaa tattgaaaag catatccaaa atttaaagaa atttatttct ctttatcttc
    11581 attttctttt ttctcttcgt tattcgatcc tgtatattca tttatcttat cttttacatt
    11641 tttaacttgt tcattatcgc tatttttaaa tttttctact gcgtctttag ctttatccat
    11701 aaaactcata ttaatcgctc ctcttatatt tgattagttt aattgaactt attttttaag
    11761 tttatcaatt gcatcagtta ttttgttttt agcattttca acaacttctt ttgctttacc
    11821 aatcacttta tcttgctgac cttctttttc taattctttg ttatcagtaa cgttacctac
    11881 tgtttcttta acatttcctt taaattgatc gaacttactt tcgtctgcca tagtgaaacc
    11941 tccttggatg tatatattta tataccacta aggaggttcg ctaaacatta taatatgaag
    12001 tttttatgtt atagtatagt atttatacga ttaaatataa aacatgtatc cgtctaaatc
    12061 ttcacttgta tctacatatt ccgctaaata tttcaatgtt gtattatcta aaacatctct
    12121 cactgcatct ctcatgcgaa tccatagttg tttttgcgca ggtggttctg attcaatact
    12181 ttcaacaaat gtaattggac cttctaacag tcttataata tcccctgctg agatttcttc
    12241 cgctggcact cttaattggt atccaccttt agcaccgcgt acacttcgaa ttaaccccgc
    12301 atttcttaaa ggacctacaa gctgttctaa atataaatca ctcaaattat tttcttcagc
    12361 aattgacttt aatgatatac atccttgccc ctctttttta gcaagagaaa tcatcaatgt
    12421 aagtccatat ctccctttag tagaaatttt cattgtataa cctcacttaa ttcgaatatt
    12481 gatattccca ttttagcatt ttttgagtta agatagtata agaaaggtgt gacaaatgtg
    12541 agtacagaac cattagcatc gagaatgcgc ccaaaaaata tagatgaaat catttcccaa
    12601 caacatttag ttggaccaag aggcattatc agaagaatgg ttgatacaaa aaaattaact
    12661 tcaatgattt tttatggtcc acctggtata ggcaaaacaa gtattgccaa agcaatttcg
    12721 ggcagtacgc aatataaatt cagacaattg aatgctgtaa ctaacactaa aaaagatatg
    12781 caacttgttg ttgaagaagc taaaatgtct ggtcaagtta tcttgttatt agatgaaata
    12841 catcgactag ataaagctaa acaagacttt ttattacctc atttagaaaa tggcaaaatc
    12901 gtcttgatcg gtgctacaac ttcaaatcct tatcatgcta tcaatccagc gattcgttca
    12961 agagcgcaaa ttttcgagtt atatccttta aatgacgaag atgtgcgcca agcgttaact
    13021 cgtgcaatag aagatgatga gaatggtttg aaaacatatc aacccaaaat tgatgaagat
    13081 gccatgacct acttttctac acaaagccaa ggtgatgttc gtagtgcgtt aaatgcattg
    13141 gaattagctg tattaagcgc agataatgac aaagacggtt atcgacatgt tacattgcaa
    13201 gatgctaaag actgtttaca aaaaggtgca tttgtaagtg acaaggatgg tgacatgcat
    13261 tacgatgtta tgagcgcttt ccaaaaatct atccgtggta gcgacgtcaa tgccgcttta
    13321 cattatttag cacgattaat tgaagctgga gatttaccta caatagttcg acgattactt
    13381 gtaattagct atgaggatat aggcttagcc tcacctaatg ctggtcagag aacacttgct
    13441 gctattgaat cagcagaacg tctaggttta ccagaagcta gaattccact aagccaagca
    13501 gtaatcgaac tatgcttatc acctaagtca aattcagcaa tgagtgccat tgatagtgca
    13561 ttgtccgata ttagaaacgg tcatgtgggc caaattccaa accatttaaa agatggacat
    13621 tatcaaggtg ctaaagatct aggccgatct attggttaca aatatccaca ccaatatgtt
    13681 aatggctatg tttcacagca atatttacct gataaactaa aaaacaaaat ttattacgaa
    13741 ccaaaaacga catctaaaag tgaacaacaa ctcaaagaaa tatataacaa cttacttaaa
    13801 caaaggccgt aaaaataaga ttaaatcgat tacctttgat attttagcta taatcaaatc
    13861 aaaactgact agtacacgag gcatattcta ctatgcttgc gaactagtca gttttagttt
    13921 tatggtgtgg ttacacttgc cttacgttga aaattatccc tttcaaaatg aattattgcc
    13981 ctttgtcttt aatgcgacga actggaatat cttttaatac gtcattcacc acataacttg
    14041 cacaaattaa tccaacaaca cttggcacaa aggcatttga agaaggtggc atttgtcctt
    14101 ttcgattgat agcattttta tctccaacta tatcttttac atcttctctt atgacaattg
    14161 gactttcatc tgaaaataca actggaatcc ctttacgaat tcctagtttt ttcaattttt
    14221 gacgaataat tttggccatt ggatcggtat gtgttttaga gatatctgaa attgtaaaac
    14281 gtgttggatc tgttttattt gcagcaccca tactggaaat cactttaatc cctcggtcaa
    14341 gacactcttt cattaagtgt actttgtaca ttattgtatc acttgcatct acaaaataat
    14401 ctatatcgta gttatcgaaa atttcttcat atgtctcttc tgtataaaac atatgtaagg
    14461 gcgtgacttt acaatctgga ttaattaatt taatacgttc ttccatcaaa gaaactttac
    14521 tttgtcctac cgttgtagtt aaagcgtgta attgtctgtt tacatttgta atatcaacat
    14581 catctttatc tattaatata atatgaccaa tattcgttct tgctaatgct tcagcagcaa
    14641 atgaaccaac acctccaacg ccaagtatga caacagtttg ttgcttcaat aaatctaaac
    14701 cttgttgtcc aatcgctagt tcatttcttg aaaattgatg tttcattatt ttacctcttt
    14761 cactgattta tacataagta catagtaact taaaatttta tatttagcat tatcactttg
    14821 attattttcc caaaattcaa cgaggaaaca tttattaaac gctataaaac ccaactaatt
    14881 ctttattaaa aacttaaaga aacgcataaa aatacgcaag acaaagtctt gcgtatcgat
    14941 agagtccgta ttgccgtagt tataatagct tgatcattcg gcctgttata tacaggtggg
    15001 tgccctgttt cttgttttgt acgtccttca tataaggcgt gtacgctgca agaaaaccca
    15061 ttgggctccc ttgatcaaag agtgttaggc ccaaattaaa aagcaaactt acgaacaact
    15121 cagatgacta tcttatgatg ttatattacc acataattaa aattaatgaa attataacaa
    15181 accaaagttt attgattttt taaaatttag tgacgaattc gcaaagaaag ttcttctaat
    15241 tgtttatcag aaacttcacc aggcgcattc gttaataaac atgtagcaga tgctgtttta
    15301 gggaatgcga ttgtatctct caagtttgtt ctattagtca ataacatgac taatcggtct
    15361 aatcctaatg caataccgcc atgtggtggt gcaccatatt taaatgcatc tagtaagaag
    15421 ccgaactgtt cttgtgcttg ttctttagta aatccaagaa cttcgaacat tttttcttgt
    15481 aactcaccat catgaattct gattgaaccg ccacctaatt cataaccatt taatactatg
    15541 tcataagcat ttgcctcagc ttcttctggc gcagtgccaa gcttagcaat atcagcttct
    15601 tttggagatg taaatggatg atgtgctgca acgtaacgtt tcgcatcttc atcatattct
    15661 aataatggcc aatctgtcac ccataagaag tttaattttg tttcatcgat taaacctaat
    15721 tctttagcta atttgacacg taatgcacct aaactttgtg caacgacatt tggtttgtct
    15781 gcaacaaaca ttactaagtc accagcttca gcaccagtta atgtaagtaa tgtttcaaca
    15841 ttttctgttt caaagaaacg tccaattgga cctgtcaaac catcttccac aactttaacc
    15901 cacgctaatc ctttagcacc atagatgttt acaaattctg ttaaagcatc catatcttta
    15961 cgagtatatt gttcagctgc acctttagcg acaattgctt taatttcacc atcattttca
    16021 acagtatctt taaatacttt aaagtccata tcacgtccta attgagaaac gtcaattaat
    16081 tccatttcaa aacgtgtatc tggtttatca gaaccatagc gacgcatcgc ttctttatat
    16141 gtcatgcgtg ggaaagcgcc attaatttca acgcctttaa cttctttaac aactttttta
    16201 agcatttctt cacccatttg catcacatct tcttggtcta caaaactcat ttcaatatcg
    16261 acttgtgtaa attcaggttg acgatctgca cgtaaatctt cgtcacggaa gcattttacg
    16321 atttggtagt atttgtcaaa tccactaatc atcaataatt gcttaaataa ttgtggtgat
    16381 tgtggtaatg cataaaattc accatcatga acacgagatg gtactaaata gtcacgtgca
    16441 ccctcaggtg ttgacttcgt tagtactggt gtttcgatgt caaagaaccc ttcatcatcc
    16501 aaatattgac gaatagaacg tgtaatttga tgtctcattt taaatgtttg cgctaactct
    16561 tgacgacgta aatctaaata acggtatttt aatcgaatat tttcatcaac gttaacattt
    16621 tcttcattta tagaaaatgg tggtgtctca gatttattaa tcactttaat atttgtaact
    16681 tgtacttcaa cttggccagt tttaatttta ggattaactg tttcagggtc acgcttcgta
    16741 actgtacctt gaacttctac aacatattca gaacgtactg tttcagcaat tttcaatgcc
    16801 tcttctgaaa atgcaggatt aaacacgact tgtacaattc cttctctatc tcttaaatca
    16861 acgaaaatca atccaccaag gtcacgacga ttgttaaccc atccttttaa tgtaatttct
    16921 tgtcctaaaa atgcctcagt aactaatcca caataagttg ttctcttact catattttaa
    16981 cgccctctct acttcttaaa atattcgact aatgcgtcta attcaattgt ttcagattca
    17041 ccagttgtca tatttttaac atcgatttta ttattttcta attcttgatc accaataacg
    17101 attgtaaact tggcacctaa acggtctgct tgtttcattt gtcctttaat tttacgctgt
    17161 aaatagtctt tatctgcttt aataccatta tgtctcaaat gatttaatag cttcacagca
    17221 tatcgatctg cttgatcacc cattgtaaca atgaataaat ctaagttttc ttcaatatct
    17281 aattcgatac cttcttcttc aagtgcaagc aataatcgtt ctatacttag cgcaaaacca
    17341 atacctgttt cacttggacc atctagcaat tctaataaac cattataacg gccaccacca
    17401 caaagcgttg taatggcacc atcatagtta gggttatcca tcattaattc aaatgctgta
    17461 tgtgtataat aatccaatcc acgaactaag ttaggatctt caatatatgg aatacctaaa
    17521 tcatctaaat aagcttttac ttgttcataa tatgccttag attcctcatt taagaaatca
    17581 gtgattctag gtgcagtctt aatcgcttct ttatcacggt ctactttaca atccaaaatt
    17641 cgcatcggat ttgtatgcaa acgtgattga caatctgaac aaaattcatg aattactggt
    17701 tcaaagtgtt tcactaacgc ttcgttatat tcttttcgag acgccatatc ccctacacta
    17761 ttaataacaa gctttaaatg ttttaatcca aatgattgat aaatatgcat aaccatagct
    17821 aatacttctg catctacgct aggattttca gcaccaatag cttctacacc aaattgatta
    17881 aattgacgat agcgtccttt ttgcttacgt tcatatctaa acatcggtcc attgtaataa
    17941 agtttaattg gttggtttgg attaccttgc attttatgtt caatatatga acgcacaact
    18001 gcagctgttc cctcaggtct taatgtaata cttctatcgc ctttatcttt aaatgtatac
    18061 atttcttttt gtacgacatc ggttgaatca ccaacacctc ttgcaaaaag atctgtactt
    18121 tcaaaaattg gtgttcttat ttctttataa ttataaaatg tcattaattc atctaattga
    18181 ttttcaatgt aacgccattt ctttgaatct tcaggtaaaa tatcctgcgt ccctctaggt
    18241 attttaatca ttttgacatc tccctcggca aactatttta tttaattacc tttttaaaac
    18301 taaaactata tacgaccact taactttgca cgataaatca taatcttaaa agcaaaagaa
    18361 aaagcccttg cacagcctga aactgcacaa gggacgaata tccgtgttgc caccctcatt
    18421 ggtttaaaaa acatgcctaa ttgtaatcat cattactttt tataagcgtg catttaataa
    18481 acccactcaa aacgtttaac gtacgtttaa cggctttact tttcataaag cacctcttct
    18541 tgtgttcaat gtatataaac tactcagcat aatctttcag tctatggatt atattcctac
    18601 acaatcttag ttagattgct acctatttca tttaccattt caagaatact aacggtttta
    18661 gttataaaat tcaatatcaa tgataactac tttcacatat ttttgcaagc cctacgcaga
    18721 aaaataaatt ttaaggccat caacaattgc ttgttctaaa atttgtctat gtaattgatc
    18781 tttaatcatc gtttcatcag ttgggttact aatataacct aattctaata aaacagcagg
    18841 aacttttgtt tgtcttaaca cttgataatt ttcttgtctt gaaccgcgat tagaaagtag
    18901 acctttcttc tgaatcgtag cgtctaacgt atctgctaaa gctctttgat tatcatgata
    18961 ccaataaact gtcattccat ttgcattaga tgattctaac gcatcattat gtatactcaa
    19021 ataggcatcg cctttgatat cacgattttc tagtgaaaca tatgtatcgt ctgttcttgt
    19081 catcttaaca gttgcgcctt ctttttctaa agtacgctgc aattcttttg ctgttttcaa
    19141 cgtataatct ttttctaaac ttttatattt agtattgctt gaagcaccct ggtcactacc
    19201 tccatgacca ggatcaagca ctattgtttt accttgcaaa ggatttttct ccttcgtatt
    19261 atccgcgaca atatctaaat ttgtgtgcca tccagctatc caaccttttt cattactgga
    19321 tgtatcttca acttcaatcc atttacctac tttaccaatc tttttaaaat ggtcaccttt
    19381 ttcaacttta tatatgactg gatacgcagc gtttggacct gtacgtaatt cagcattttc
    19441 agttatcgtg atgttcccac tatcttcact attgctattc agcaataaaa ataaaaagat
    19501 gataaataag acaaaggcaa tcactactat tagagtacgt ttatttttaa gacccttttt
    19561 agataaccat gcctctattt ttttcattga attttgccgt cctgactttc ataaataata
    19621 gtgactggac catcattatt tatgctaaca ttcatgtgtg ttccaaattc acctgttttc
    19681 acagtaagac catacgctcg tagcgcatca ttaaaatact cataaatttt taccgcttga
    19741 tcaggatttt tagaatttga gaaacctgga cggttacctt tttttacatc tgcatagaga
    19801 gtaaattgtg aaactgatag tatttcacca ttcatttgtt ggatattaaa gtttaattta
    19861 ttattgtcat cttcaaataa tcttgcatta gcaattttct ttgcaattac atctgcatct
    19921 tgctctgtag agttctgacc gataccgact aataaacaat atcctttttt gatttgatta
    19981 tttaatgtat cattcgtcac cgatgcttct ttaactcttt gtacaactac tttcatattt
    20041 tgcacctcta gttccaaact cttgttactg tataaacatc accaagttgt ttgatctttt
    20101 ctaccacacg ataaacatca ttcacgtttt tcaccatgac actaatattt attattgcat
    20161 ttttatcaat atctgaacgt cctgaaactt taattaaatt gccggctgtc gagctaacag
    20221 cttgtagtac ttcattcaac aagccatttc ggtcatacgc agttacctct aaatcaacct
    20281 gatatttttg agttgcgtct tttgatttta cccattcaac attaattagt cgttcagttt
    20341 cgttcttaat atttgggcaa tcagtgcgat gtactttaat accgtgacct ttggtgatat
    20401 aacctacaat atcatcacct ggtataggat tacaacattt tgacaacttg ataagtacat
    20461 tttctaaacc ttctacatag acaccactat cagtaatgat gttgtcttta ataggcaatg
    20521 atttcgtaac ttcttgtgct tcatttaaag cacgttgttt atctaaaata cgttgtcttt
    20581 cagttaattt attaacaatc tgtaaggatg tcacgccgcc aaatcctaca gctgcgaata
    20641 aatcatcttc atttgcaaag ttatattttt cattaacaac ctgaatattt ttctctgtca
    20701 aaatatcttc gactctaaat ccttgctctt ttatttcagc ttcaaccatc attcggcctt
    20761 tttcaatatt agatgaacga tcttgttttt tgaagaaact tttaatttta cctttggcac
    20821 tagacgattt aacaattttc aaccaatcac gacttggtcc atatgaatgt ttactagtac
    20881 gtatttcaac aatatcgcct gtttgtaaaa tatagtcaat tggtacaatt ttgccattca
    20941 ccttggcacc aatcatctta ttacctactt cactgtgaat cgcataagca aaatcaatcg
    21001 gcacagcacc atatggcaac tcaataacat cactcgctgg ggtaaatgcg tatactttgt
    21061 cactctgtaa gtcatatttt aaggtttcca taaattcttg agcgtcagac gatgtatgat
    21121 ccgcttcagc taattctttt aaccaattta acttattttg ataagtttga tctttttcac
    21181 ttactttttt accttctttg taagcccagt gtgctgcaac accatgctca gcaatttcgt
    21241 gcatatcaaa cgttcgtatt tggatttcga gcgggtctcc atttgggcct actactgtag
    21301 tatgcaatga ctgatacaaa ttttgtttag gcattgcaat ataatcttta aaacgtcctg
    21361 gcatcggttt ccataacgta tgcaccaacc caagtatcgc ataacaatca ttaatagaat
    21421 tgacaataac acgtatcgcc aacaaatcaa aaatttgatc aaattgtttt ttctgcttca
    21481 tcattttccg ataaatactg taaatatgtt taggtctacc atttatatcg ccttcgatat
    21541 tcattcggtc catttcagta cgtattctat caatagccgt ttcgatatac gcttcacgtt
    21601 cactacgttt cttcttcatt aaattgacta ttctaaaata ttgcacatta tcaatataac
    21661 gaagagccgt atcttctagt tcccatttga ttgtattaat accaagacga tgtgctaatg
    21721 gtgcataaat ttctaatgtt tctcgagaaa ttctaatttg tttttcgcgc ggcatggctt
    21781 tcaaggtacg catattatgt aatctgtctg ctaatttcac caaaattacg cgtacatctt
    21841 tggcaatcgc aataaataac ttgcgatgat tttcagcttg ttgttcttct tttgagcggt
    21901 attttacttt tttaagcttc gtcacaccat caacaattcg agcaacttct tcattgaaca
    21961 tttcttttac atcttcaaat gtatacggtg tatcttcaat tacatcatgc aaaaaacctg
    22021 cgacaatcgt cggtccgtct aatcgcattt ctgttaaaat acctgcaact tgtataggat
    22081 gcataatgta tggtaatccg ttttttcgga actgaccttt atgtgcttca taagcaatat
    22141 gatagctttt taaaacatac tcatattcat ctgctgacaa atatgatttt gctttgtgaa
    22201 gaacttcgtc tgcactatat ggatattcgt tgttcattat atgatacacc ccattcatat
    22261 ttattacttc gccttaaaca atgatttagg tactcttgtt gaatagtatt tgtcccacac
    22321 caatcatacg tccgtcgacg ataaatattt atcctgtcgt cattaatcgt aatattaatt
    22381 ttacttgagc gagtttaatt tgtatactat tcctactttt aaaactttta caaaaattcg
    22441 acctaaatct actgtttcat tttttaaata ttagttctat gatactacaa tttatgaaat
    22501 aaataaacga tgttattaag gtataatgct caatcatcta acattttcag taaataaaaa
    22561 atcctaacat ctcatgttaa gaaaacttaa acaact
//
LOCUS       DALAGD010000047        22434 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.047, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000047 DALAGD010000000
VERSION     DALAGD010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22434
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.047"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(240..905)
                     /locus_tag="O2701_001421"
     CDS             complement(240..905)
                     /locus_tag="O2701_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4293320.1"
                     /translation="MALVVEDIVKNFGEGLSETKVLKGINFEVEQGEFVILNGASGSG
                     KTTLLTILGGLLSQTSGTVLYNDAPLFDKQHRPSDLRLEDIGFIFQSSHLVPYLKVIE
                     QLTLVGQEAGMTKQQSSTRAIQLLKNIGLEDRLNVYPHQLSGGEKQRVAIMRAFMNNP
                     KIILADEPTASLDADRATKVVEMIRQQIKEQQMIGIMITHDRRLFEYADRVIELEDGK
                     ITD"
     gene            complement(905..1960)
                     /locus_tag="O2701_001422"
     CDS             complement(905..1960)
                     /locus_tag="O2701_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4293321.1"
                     /translation="MKLAIKEIMFYKFRYILITLIILLLSIMVLFISGLAQGLGRENI
                     SLFEHFDNDEYVVQKMKEPQIEKSQLSDTQQNQIKKVIHQEPYKMNIQTLKLSNKEQD
                     VITMNDVKQQRIQLKKGDYPKNAHEVAINDKLAADNIRVGDRLHFKNNSTSYRVSGIL
                     NDTMYAHSSIVLLNDNGFNALNKVNTAFYPVKNLTQQQRDELNKINDVQVVSEKDLTG
                     NIASYQAEQAPLNMMIVSLFAITAIVLSAFFYVMTIQKISQIGILKAIGIKTRHLLSA
                     LVLQILTLTIIGVGIAVIIIVGLSFMMPVTMPFYLTTQNILLMVGIFILVAILGASLS
                     FIKLFKVDPIEAIGGAE"
     gene            2096..2770
                     /gene="hssR"
                     /locus_tag="O2701_001423"
     CDS             2096..2770
                     /gene="hssR"
                     /locus_tag="O2701_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding heme response regulator HssR"
                     /protein_id="HCZ4293322.1"
                     /translation="MVQCLVVDDDPRILNYIASHLQTEHIDAYTQPSGEAALKLLEKQ
                     RVDIAVVDIMMDGMDGFQLCNTLKNDYDIPVIMLTARDALSDKERAFISGTDDYVTKP
                     FEVKELIFRIRAVLRRYNINSNSEMTIGNLTLNQSYLELQVSNKTMTLPNKEFQLLFM
                     LAARPKQIFTREQIIEKIWGYDYEGDERTVDVHIKRLRQRLKKLNATLTIETVRGQGY
                     KVENHV"
     gene            2763..4136
                     /gene="hssS"
                     /locus_tag="O2701_001424"
     CDS             2763..4136
                     /gene="hssS"
                     /locus_tag="O2701_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme sensor histidine kinase HssS"
                     /protein_id="HCZ4293323.1"
                     /translation="MFKTLYARIAIYSITVILFSALISFVLTNVYYHYNLKASNDAKI
                     MKTLKEARQYEQSAKPTHIQQYFKHLGQMNYQIMTIDQKGHKTFYGEPFREDTLSQNA
                     INNVLNNQDYHGIKDKPFALFVTGFFDNVTDNTVGINFKTKDGSIAVFMRPDIGETFS
                     EFRTFLAVLLMLLLFISISLVIASTYSIIRPVKKLKLATERLIDGDFETPIKQTRKDE
                     IGTLQYHFNKMRESLGQVDQMRQHFVQNVSHEIKTPLTHIHHLLSELQQTSDKTLRQQ
                     YINDIYTITTQLSGLTTELLLLSELDNHQHLLFDDKIQVNQLIKDIIRHEQFAADEKS
                     LIILADLESINFLGNQRLLHQALSNLLINAIKYTDVGGAIDIALQHSHNNIIFTISND
                     GSPISPQAEARLFERFYKVSKHDNSNGLGLAITKSIIELHHGTIQFTQSNEYVTTFTI
                     TLPNNSL"
     gene            4299..4742
                     /locus_tag="O2701_001425"
     CDS             4299..4742
                     /locus_tag="O2701_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCZ4293324.1"
                     /translation="MMKLNLFINANETESYIDIHAPKMNDNVQSIINAVNDLDKSHTL
                     VGYIDKEIHIINVSDVITFQVINKNVTAITSNQKFKLKLRLYELEKQLPQHFIRISKS
                     EIVNKYYIEKLLLEPNGLIRMYLKDAHYTYSSRRYLKSIKERLSI"
     gene            4739..5194
                     /locus_tag="O2701_001426"
     CDS             4739..5194
                     /locus_tag="O2701_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HCZ4293325.1"
                     /translation="MKNLKNSLFISLIIGLSLSLFFSMLFADGKYYPLNPQSTIGILY
                     YTHFTETTVMLISIILWLLIGVVFFLGDFIFKYTDWSITKATIMHFITTYVGFLPLAM
                     LAGWFPLTVHYLIIFTIIFIVVYVLIWIIQFFKNKNYVDTINKQLKQLK"
     gene            complement(5260..6738)
                     /gene="mqo"
                     /locus_tag="O2701_001427"
     CDS             complement(5260..6738)
                     /gene="mqo"
                     /locus_tag="O2701_001427"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCZ4293326.1"
                     /translation="MTTQHSKTDVILIGGGIMSATLGTLLKELSPEKNIKVFEKLAQP
                     GEESSNVWNNAGTGHSALCELNYTKEGKDGTVDCSKAIKINEQYQISKQFWAYLVKTG
                     QLDNPDRFIQAVPHMSFVIGEDNVAFIKSRVATLKKSILFEKMKLSQDEEEMKSWVPL
                     MIEGRKSDEPIALTYDETGTDVNFGALTAKLFDNLEQRGVGIQYKQNVLDIKKQKSGV
                     WLVKVKDLETNETTTYESDFVFIGAGGASLPLLQKTGIKQSKHIGGFPVSGLFLRCTN
                     QEVIDRHHAKVYGKAAVGAPPMSVPHLDTRFVDGKRSLLFGPFAGFSPKFLKTGSHMD
                     LIKSVKPNNIVTMLSAGIKEMSLTKYLVSQLMLSNDERMDDLRVFFPNAKNEDWEVIT
                     AGQRVQVIKDTEDSKGNLQFGTEVITSDDGTLAALLGASPGASTAVDIMFDVLQRCYR
                     DEFKGWEPKIKEMVPSFGYRLTDHEDLYHKINEEVTKYLQVK"
     gene            complement(7119..8717)
                     /locus_tag="O2701_001428"
     CDS             complement(7119..8717)
                     /locus_tag="O2701_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCZ4293327.1"
                     /translation="MLVNTFNPFDNLLLSSLIAAIPIVLFLLCLTVFKMKGIYAAITT
                     LVVTLLIAIPFFKLPVGIASGAVVEGFFQGIIPIGYIVMMAVLLYKITVESGQFLTIQ
                     DSITNISQDQRIQVLLIGFAFNAFLEGAAGFGVPIAICALLLTQLGFNPLKAAMLCLV
                     ANAASGAFGAIGIPVGVVETLKLPGDVSVLGVSQSATLTLAIINFIIPFLLIFIIDGF
                     RGVKETLPAILVVSITYTFTQGLLTVFSGPELADIIPPLLTMLALAVFSKKFQPKHIY
                     RVNKDEEIEPAKAHSAKAVLHAWSPFIVLTVIVMIWSAPFFKNLFLPNGALSSLVFKF
                     NLPGTISEVTHKPLVLTLNIIGQTGTAILLTIIITILMSKKVNFKDAGRLFGVTFKEL
                     WLPVLTICFILAISKITTYGGLSAAMGQGIAKAGNVFPVLSPILGWIGVFMTGSVVNN
                     NSLFAPIQASVAQQIGTSGSLLVSANTVGGVAAKLISPQSIAIATAAVKQVGKESELL
                     KMTLKYSVCLLIFICIWTFILSLL"
     gene            9034..10707
                     /locus_tag="O2701_001429"
     CDS             9034..10707
                     /locus_tag="O2701_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCZ4293328.1"
                     /translation="MIKQINVSNMQKFESQLMKAQSEGYTHVVPYANEIMIYQSMLDA
                     VQLYPKSIVVDYTVDGQYKNDCHYFGQSSINIADWAQNNNYYPNLIYAIQQTLDLIHY
                     YSVETIFDLALLTLLKGDLSIDGHVVFDFKAPIATSASIWETIKTIEDFDMMSQFYLN
                     KMAYIDHHPIPFRNLFIEDSEQLNSPDNWLYSTKFMLPKWLYKIAKQRADNKQLQNFG
                     LYTKQPNVLKDHIVFIGDHHQYIGNSKYLFTYFVKHNPMTACYFVTDDRRGPHFISPK
                     SEKADELINSARVVIVENDIPETLQPNGTLIQLHQGTPIMQLFLDSKEPIKNIETPFY
                     RAKRYNRWLQFDYVIHSADDISHFYQTAFPSHQANVLAYGNPKHQYLLQKSNESTTQQ
                     QYKKSFKINDQKPVLLYAPIGLVSAQQLPLSDALFKAYHVVVQGVDEAILPEEALVAP
                     KYLSAQDLILMSDVVITDYSNIIFDAMAIDKTVALYTPNHSQYIESQGVNEDIWRHLS
                     KIWYTDRQLLINNLISQAIPVIKYPQIQHKEQPLESISQLILSKMTSNQ"
     gene            complement(10860..11489)
                     /locus_tag="O2701_001430"
     CDS             complement(10860..11489)
                     /locus_tag="O2701_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293329.1"
                     /translation="MKRLVTGLLALSLFLAACGQDSDQQKDGNKEKDDKAKTEQQDKK
                     TNDSSKDKKDNKDDSKDVNKDNKDNSANDNQQQSNSNATNNDQNQTNNNQSSNNQANN
                     NQKSSYVAPYYGQNAAPVARQIYPFNGNKNQALQQLPNFQTALNAANNEANKFGSNNK
                     VYNDYSIEEHNGNYKYVFSFKDPNANGKYSIVTVDYTGQAMVTDPNYQQ"
     gene            complement(11803..12318)
                     /locus_tag="O2701_001431"
     CDS             complement(11803..12318)
                     /locus_tag="O2701_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4293330.1"
                     /translation="MAGIIKEISEQNASELVELATRTFYDTFGSYYDDKDFDQFFKDN
                     YTVEKFTQEINHVDSFHYFYQEDGANVGYIKMNINSAQTEEMGETYLEVQRIYFLKDF
                     QGGGRGSQLIELAEKIAQEHNKHKIWLGVWEHNPRAQAFYKRHGFKVVGEHHFQTGDV
                     TDTDLIMEKEL"
     gene            12509..13513
                     /locus_tag="O2701_001432"
     CDS             12509..13513
                     /locus_tag="O2701_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCZ4293331.1"
                     /translation="MIETFKAFVIDKDESGKVTPTFKQLSPTDLPKGDVLIKVHYSGI
                     NYKDALATQDHNAVVKSYPMIPGIDLAGTIVESEAPGFEKGEQVIVTSYDLGVSHYGG
                     FSEYARVKSEWIIKLPDTLTLEESMIYGTAGYTAGLAIERLEKVGMNIEDGPVLVRGA
                     SGGVGTLAVLMLNELGYKVIASTGKQDVSDQLLELGAKEVIDRLPVEDDHKKPLASST
                     WQACVDPVGGEGINYVTKRLNHSGSIAVIGMTAGNTYTNSVFPHILRGVNILGIDSVF
                     TAMKLRQRVWRRLAKDLMPENLHEIKQVITFDELPEQLNKVIKHENKGRIVIDFGVDK
                     "
     gene            complement(13559..13960)
                     /locus_tag="O2701_001433"
     CDS             complement(13559..13960)
                     /locus_tag="O2701_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4293332.1"
                     /translation="MVKVTYDIPTCEDYCALRINAGMSPKTREAAEKGLPNALFTVTL
                     YDKDRLIGMGRVIGDGGTVFQIVDIAVLKSYQGQGYGSLIMEHIMQYIKGVAVESTYV
                     SLIADYPADKLYTKFGFIPTEPDSGGMYIKY"
     gene            complement(13996..15030)
                     /locus_tag="O2701_001434"
     CDS             complement(13996..15030)
                     /locus_tag="O2701_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCZ4293333.1"
                     /translation="MKDVTIIGGGPSGLYASFYAGLRDMSVRLIDVQSELGGKMRIYP
                     EKIIWDIGGIAPKPCHEILKDTIKQGLYFKPEVHLNERVVDIRKKAERHFEVETEAGE
                     IYTSKAVIIAIGAGIINPKQLDVKGVERYQLTNLHYVVQSYRRFKDKDVLISGGGNTA
                     LDWAHDIAKIAKSVTVVYRKEDVSGHEAMKTLVTDLNVKLCPKTRIKYLVGNDDETHI
                     SEVVLEHVESGDRHTVKFDDVIISHGFDRCNTLLSETSSKLDMHDDCRVKGFGNTTTS
                     IPGIYACGDIVYHDAKSHLIASAFSDGANAANLAKTYIQPDANAEGYVSSHHEVFKEA
                     NKTIVNKHLY"
     gene            complement(15041..15157)
                     /locus_tag="O2701_001435"
     CDS             complement(15041..15157)
                     /locus_tag="O2701_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293334.1"
                     /translation="MANYAIYFNNININSIMALNDNNYQFEHVNERNEYRES"
     gene            complement(15262..15693)
                     /locus_tag="O2701_001436"
     CDS             complement(15262..15693)
                     /locus_tag="O2701_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2871 domain-containing protein"
                     /protein_id="HCZ4293335.1"
                     /translation="MRRLLYSFLFYMIIGLFSGFFYRELTKAYDFTGTTQLSLVHTHT
                     LILGMFMFLILLPLEKLFKLSSYYLFNWFFYVYNIGVIVTIGMMVTKGFFQVTGKSYS
                     PEAFAGFAGIGHTGMLAGLLLLFFLLRQAILKEPRDESFKK"
     gene            complement(15900..17177)
                     /locus_tag="O2701_001437"
     CDS             complement(15900..17177)
                     /locus_tag="O2701_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCZ4293336.1"
                     /translation="MNIFKNKLLWIAPIATMIILVIFSLAFYPAYNPKPKDLPIGILN
                     EDKGTTIQDKNVNIGKKLEDKLLDSDSNKIKWVKVDSEKDLEKDLKDQKIFGVAIIDK
                     DFSKDAMSKTQKVVMDSKKEEMQQKVASGEIPPQVVQQMKQKMGNQQVEVKQAKFKTI
                     VSEGSSLQGSQIASAVLTGMGDNINAQITKQSLETLTSQNVKVNAADINGLTNPVKVD
                     NEKLNKVKDHQAGGNAPFLMFMPIWIGSIVTSILLFFAFRTSNNIVVQHRIIASIGQM
                     IFAVVAAFAGSFVYIYFMQGVQRFDFDHPNRIAIFVAFAILGFVGLILGVMVWLGMKS
                     VPIFFILMFFSMQLVTLPKQMLPESYQKYVYDWNPFTHYATSVRELLYLNHHIELNST
                     MWMFIGFMIFGAVSSLVSAIVRKHSTKRTEVPS"
     gene            17338..17961
                     /locus_tag="O2701_001438"
     CDS             17338..17961
                     /locus_tag="O2701_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCZ4293337.1"
                     /translation="MKEDRRIRKTKSSIKQAFTKLLQEKDLEKITIRDITTRADINRG
                     TFYLHYEDKYMLLADMEDEYISELTTYTQFDLLRGSSIEDIANTFVNNILKNIFQHIH
                     DNLEFYHTILQLERTSQLELKINEHIKNNMQRYISINHSIGGVPEMYFYSYVSGATIS
                     IIKYWVMDKQPISVDELAKHVHNIIFNGPLRIMAENRLHKSNLDSLT"
     gene            18007..18951
                     /locus_tag="O2701_001439"
     CDS             18007..18951
                     /locus_tag="O2701_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCZ4293338.1"
                     /translation="MITSFRHSEDIDKHIIKTPLDHTASWINVVEPDREEIENLMEQY
                     NIPEDFIRDPLDSEESSRIEYDEDTGYSLIIIDLPIVNSTNRSVLSFVTIPLGIIIGN
                     GIIVTVCDAENEFLENLPKRDINLKFHSRFALEILTTIADHYNRNLRLLNKSRIRIEK
                     ELKNNITNKQLFKLMEVEKSLVYFLAALKGNDTIIKKLFRLPAIKRFEEDEELLEDLI
                     IENNQAIETTELHQRILESITTSYASLLSNDMNTIMKTLTLFTVLLTLPTLVFSFFGM
                     NVPLPIDDHSYISWIIVVGISLILVVIVSIFLWRKQKL"
     gene            complement(19037..20479)
                     /locus_tag="O2701_001440"
     CDS             complement(19037..20479)
                     /locus_tag="O2701_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HCZ4293339.1"
                     /translation="MNYKQSAEEILNAIGGEENLDAMAHCATRLRLVLNDESLVNEEA
                     LNNMDVVKGTFSTGGQYQIIIGSGTVNKVFSELEKLTGKEASTTSEVKAQSAKNMNPL
                     QRFVKMLSDIFVPIIPAIVAGGLLMGLNNILTAKDLFFSGKSLIDVYSQFAGLAEMIN
                     VFANAPFTLLPILIGFSAAKRFGGNPFLGAALGMILVHPSLMSAYDFPKAVEAGKAIP
                     YWDVFGLHINQVGYQGQVLPMLVAAYILASIEKGLRKVIPTVLDNLLTPLLSIFITAF
                     LTFSFVGPITRQLGYWLSDGLTWLYEFGGAIGGLIFGLLYAPIVITGMHHSFIAVETT
                     LIADATKTGGSFIFPIATMSNVAQGGAAIAAFFIIKQNKKLKGVASAAGISALLGITE
                     PAMFGVNLKLRYPFIGAIVGSGIGSAYIAFFKVKAIALGTAGLPGFISINPVHAGWLH
                     YFVGMTISFIIAITVTLILSKRKANKEVVE"
     gene            20662..21045
                     /locus_tag="O2701_001441"
     CDS             20662..21045
                     /locus_tag="O2701_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCZ4293340.1"
                     /translation="MKERGYIEQLWREEKYHVLLHSQQSYQMIRNALKTDLSLHQVQQ
                     MIDVALLIEPSIGSVCNAFDHMWGYLKKCANEEERQQSKLLKADFINGKIDTQTLLDF
                     LAELANKYDVQYLLQSRVLNTKRKR"
BASE COUNT         7544 a         3930 c         3226 g         7734 t
ORIGIN      
        1 taatgataga tatggtttta aacgtgactt caagctatat gaatgcaacg agctgtacat
       61 tataaaatac atatcaaaaa agctgatttc tatcaaataa ttaatagaaa tcagcttttt
      121 tacattgcct aagaacttaa tgtcccagcc tgttttttgt aaacccaaat cgtattctgt
      181 tatctaactt ttatactact atgtcttaaa tcattaacat ttagcgtctt tacaagccac
      241 taatcagtta ttttgccatc ttctaattca atcactcgat ctgcatattc aaataatctt
      301 cgatcgtgtg taatcataat accaatcatt tgttgttctt taatttgttg acgtatcatc
      361 tcaacaactt ttgttgctct atcggcatct aaacttgctg tgggctcatc tgctaaaatg
      421 attttcggat tattcataaa tgctctcata atcgcaacac gttgcttttc accgccagat
      481 aactgatgcg gatatacatt caagcgatct tctaaaccaa tatttttcaa aagttgtatt
      541 gctcttgttg aactttgttg tttggtcatt cccgcttctt gacctacgag tgtcaattgc
      601 tctatcactt ttaaataagg aactaaatgt gaagattgaa aaataaaacc aatatcttcc
      661 aatcgtaaat cactaggacg atgctgttta tcaaacaatg gcgcatcatt gtaaagcact
      721 gtaccactcg tttgacttaa caatccgcct aatatcgtta gcaatgttgt tttcccagaa
      781 ccagaggcac catttaaaat gacaaattcc ccttgttcca cttcaaaatt aatacctttt
      841 aaaacttttg tttcagacaa accttctccg aaatttttga cgatatcttc aacgactaat
      901 gccattattc tgcacctcca attgcttcga taggatccac tttaaataat ttgataaatg
      961 atagtgaggc acctaaaatc gctactaata taaatatccc caccattaat aaaatatttt
     1021 gcgttgttaa gtaaaaaggc atcgttaccg gcatcataaa tgatagtcct actatgatga
     1081 tcacagcaat acctacccca attattgtta gtgttaaaat ttgtaaaact aacgcactca
     1141 ataaatgtct tgtcttaata ccaattgctt tcaaaatgcc aatttgtgat attttttgaa
     1201 tcgtcataac atagaaaaat gcacttagaa cgattgctgt aatagcaaac aaactaacaa
     1261 tcatcatatt taacggtgct tgctctgctt gataactcgc aatattacct gttaaatctt
     1321 tttcactcac aacttgaacg tcatttattt tattaagctc atcacgttgt tgttgtgtta
     1381 aatttttcac tggataaaat gccgtattaa ccttattcaa tgcattaaat ccgttatcgt
     1441 tcaatagcac aatggaacta tgcgcataca ttgtgtcgtt taaaatacca gaaactctat
     1501 aactagttga attattttta aaatgtaatc tatccccgac tctaatgttg tctgcagcta
     1561 acttatcatt aatagcgacc tcatgagcat ttttaggata atcacctttt ttaagttgta
     1621 tgcgttgttg tttaacgtca ttcattgtaa tcacatcttg ttctttatta gataatttca
     1681 atgtttgaat attcatttta tatggttctt gatgaattac ctttttaatt tgattctgtt
     1741 gcgtatcact aagttgcgat ttctcaattt gcggctcttt cattttttga acaacatatt
     1801 catcattatc aaagtgttca aataacgaaa tattctccct accaagccct tgagctaaac
     1861 cactaataaa taacaccata atacttaata aaagaatgat taatgtgatt aaaatataac
     1921 gaaatttgta aaacataatc tcttttatcg ctaatttcat atcgattcac ttctccctat
     1981 ttcttcttta gcgtcagtat aaggttgaaa tatgaactca atatgaacaa ttgttttttt
     2041 acgcaaaaat attttacatg tataataaat gaaaaagata agggagttta tagctatggt
     2101 gcaatgtctt gttgtcgatg acgatcctcg aatcctaaat tatatagcta gccatttaca
     2161 aacagagcac attgatgcat acacacaacc aagtggtgaa gctgcattaa aacttttaga
     2221 aaaacagcgt gtcgatattg cagtggtaga tattatgatg gatggtatgg acggctttca
     2281 attatgtaat acattaaaaa atgattatga tataccagtt attatgttaa cagcgcggga
     2341 tgcacttagt gacaaagagc gtgcgtttat aagcggtact gacgattatg taaccaaacc
     2401 ctttgaggtt aaggaactta tttttagaat tcgtgctgta ttacgtcgat ataatatcaa
     2461 ttcaaattca gaaatgacta ttggcaactt aacgctaaac caatcctatt tggaactcca
     2521 agtatctaat aaaacgatga cgttaccgaa caaggaattt caattattat ttatgcttgc
     2581 agcacgtcct aagcaaatct ttactcgtga acaaataata gaaaaaattt ggggctatga
     2641 ttatgaagga gatgagcgaa cagttgacgt tcatattaag cgactacgcc aaagattaaa
     2701 aaaattaaat gccacactta caattgaaac agtaagagga caaggctata aggtggagaa
     2761 tcatgtttaa aacactctat gctagaattg cgatttattc cattacggtt attttattta
     2821 gtgcattaat aagttttgta ttaacaaatg tttactatca ttataattta aaagcatcta
     2881 atgacgcgaa aatcatgaag acgcttaaag aagcaagaca atatgaacaa tcagctaaac
     2941 caacgcacat tcaacaatat ttcaaacatt tagggcaaat gaattaccaa ataatgacca
     3001 ttgatcaaaa aggtcataag acattttatg gggagccatt tagagaagat actttatcac
     3061 aaaatgctat taacaacgta ttaaataatc aagattacca tggtattaaa gataaaccat
     3121 tcgcattatt cgtaactgga tttttcgaca atgtaacaga taatactgtt ggtattaact
     3181 ttaagacaaa agacggttca atagcagtat ttatgcgtcc agatattgga gaaacattta
     3241 gcgagtttag aacattctta gccgtcttgt taatgttgtt attatttatc tcgatttcat
     3301 tagttatcgc atcaacctat tcaatcattc gtccagtaaa aaagttaaag ctagcgaccg
     3361 aacgcttaat tgatggtgat tttgaaacac ctatcaaaca aacacgcaaa gatgaaattg
     3421 gaacattaca gtatcacttt aataagatga gagagtcatt gggacaagtt gatcaaatga
     3481 ggcagcattt tgtacaaaat gtatctcatg aaatcaaaac accattaaca catattcatc
     3541 atttattaag cgagttacaa caaacttctg ataaaacatt aagacaacaa tatataaatg
     3601 atatctatac cattacaacg cagctgagcg gattaacaac tgaattgttg ctcttatccg
     3661 aattagataa tcaccaacat ttattattcg atgataagat tcaagtcaat caactcatta
     3721 aagatatcat tcgacatgaa caatttgctg ctgatgaaaa gtcgttaatc attttagctg
     3781 acctagaatc aatcaatttc ctaggaaatc aacgtttgtt acatcaagca ttgagtaatt
     3841 tattaataaa tgctattaag tatacagatg ttggtggtgc cattgacatc gctttgcaac
     3901 atagtcacaa caatatcatt ttcacaataa gtaatgatgg ttcaccaatt agtccacaag
     3961 ctgaagcacg tttatttgaa cgtttttata aagtgagcaa acatgataat agcaatggtt
     4021 taggtcttgc aattacaaaa tccatcattg aattgcatca tggtacaatc caattcacac
     4081 aaagtaacga gtatgtaacc acctttacta ttacactgcc aaataattca ctttaatctc
     4141 tacctcctga aaaaacattt ctgtttttat ttcaggaggt atcttttata ttgattagcg
     4201 ctttctagcc acttaacatt taatttctac cattcaacaa aatttaaata tatcgatggt
     4261 catttatggt ttaataattt tgaaggaggc attaacctat gatgaaactc aatttattta
     4321 tcaatgcaaa tgagacggaa tcttatattg atatacatgc acctaaaatg aatgataacg
     4381 ttcaaagtat tattaatgca gtcaatgatt tagacaaatc acatacatta gtcggataca
     4441 tagacaaaga aatccatatc attaatgtat cagatgttat aacattccaa gttattaata
     4501 aaaatgtaac agccattaca agcaatcaaa aattcaaact aaaattacga ctttatgaac
     4561 tagaaaaaca gttaccacaa catttcattc gcatttccaa atcagaaatc gtcaacaagt
     4621 attacattga aaaattatta ttagagccaa atggacttat acgtatgtat cttaaagatg
     4681 cacactacac ctattcttct agacgttatt taaaatctat aaaggagcgc ttatcaatat
     4741 gaaaaatttg aaaaactcat tatttattag cttaatcatt ggtttgtccc tttcactgtt
     4801 ctttagtatg ttatttgcag acggcaaata ttatccactg aacccacaat caactattgg
     4861 catattgtat tatacgcatt tcacagaaac taccgtaatg ttgatttcta tcattttatg
     4921 gttactcata ggcgtcgtct ttttccttgg agattttatc tttaaataca cagattggag
     4981 cattactaaa gcaactataa tgcatttcat aacaacgtat gtcggattct tgcctttagc
     5041 aatgcttgcc ggttggtttc cactaacagt gcattatcta atcatattca caattatctt
     5101 cattgtggtt tatgtattaa tttggataat tcaattcttt aaaaacaaga actacgtaga
     5161 cactattaac aagcaactga aacaattaaa atgattttta ttaaccacta aaaaacggta
     5221 atgtctatca cattaaaaaa gacattaccg tttcgtttat tatttaactt gtaaatactt
     5281 agttacttct tcattaattt tatgatataa atcctcatga tctgttaagc gataaccaaa
     5341 tgacggcacc atttctttaa tctttggttc ccatcctttg aattcatcac gatagcatct
     5401 ctgtaaaaca tcaaacataa tatctacagc tgttgacgca ccaggtgatg caccaagtaa
     5461 tgcagctaat gtgccatcat ctgacgtaat aacttcagta ccaaattgta agttaccttt
     5521 agaatcctca gtatccttga ttacttggac acgttgccct gctgtaatca cttcccaatc
     5581 ttcattttta gcatttggga aaaagactct taaatcatcc atacgctcat cattagataa
     5641 catcaattgt gacactaaat acttcgtaag actcatttct ttgatacctg cagataacat
     5701 cgtcacgata ttatttggtt taaccgattt aattaaatcc atatgtgaac ctgtttttaa
     5761 aaatttaggt gagaaacctg caaatggacc aaataacaat gaacgcttgc cgtctacaaa
     5821 acgtgtatct aagtgcggca ctgacattgg tggcgcaccc actgctgctt ttccgtacac
     5881 tttagcatga tgacgatcaa tcacttcttg atttgtacag cgcaggaata atccacttac
     5941 cgggaaacca ccaatatgtt ttgattgttt aatcccagtc ttttggagta atggtaaact
     6001 cgcaccgcca gcaccaataa atacaaaatc agattcatat gttgtcgttt cattagtttc
     6061 taaatcttta actttaacta gccatacccc agatttctgt ttcttgatgt ctaatacatt
     6121 ctgcttatat tgaattccca caccacgttg ctctaaatta tcaaataact ttgcagttaa
     6181 cgcaccaaag ttaacatctg taccagtttc atcataagtt aaagcaattg gttcatcaga
     6241 cttacgacct tcaatcatta acggtaccca agatttcatt tcttcttcat cttgcgataa
     6301 tttcattttt tcgaataaaa tacttttctt taacgttgca acacgacttt ttataaaagc
     6361 tacattatct tcgccaatga caaaactcat gtgtggcacc gcttgaataa agcgatctgg
     6421 gttatctaat tgtcctgttt taactaaata tgcccaaaac tgttttgaaa tttggtactg
     6481 ctcatttatc ttaattgctt tactacaatc aactgtgcca tccttacctt cttttgtata
     6541 gttcaactcg caaagtgctg aatgccctgt accggcatta ttccatacat ttgaactctc
     6601 ttcgccaggt tgtgctaatt tttcaaacac tttaatattt ttctcaggtg ataattcttt
     6661 aagtaatgtt cctaatgttg cactcataat accgccacca attaagatga catctgtttt
     6721 gctatgttgt gttgtcataa caaatacagt cccccttttt aaaatgaata atggaacgtt
     6781 cgagaaatac gacatattaa taccacttta aatcaataag tgtctcaata ggttcccatg
     6841 gtgatgctct attactatct actatgtata ttaatatttt ataataacga aactacttta
     6901 attatatact ttatgtgcga tagcacaaag acaaaaccac aaaccttaac agctatcaac
     6961 caatttaatt tgattgctga ttactcacta ttcattttaa cataaagcgc taccattatt
     7021 ataatgtcaa acttctttca ttaatgatcc ataaaaaaaa gatgagatgt tagaaaacat
     7081 ctcaccttct gtatatttat attttgaaat acgttttttt ataataatga caagatgaaa
     7141 gtccaaatac agatgaatat tagtaaacat acactgtatt tcaatgtcat ttttaataat
     7201 tctgattcct taccaacttg ttttactgct gcagttgcaa ttgcaattga ttgtggtgaa
     7261 atcaattttg ccgctacacc accaactgta ttagcagata caagaagtga accacttgtt
     7321 ccaatttgtt gtgcaacaga agcttgaatt ggtgcaaata atgagttgtt atttacaact
     7381 gatcctgtca taaacacacc tatccaacct aaaattggtg atagaactgg gaagacatta
     7441 cctgctttag caataccttg acccattgct gcacttaaac caccataagt tgtgatttta
     7501 gaaattgcta agatgaaaca aattgtaaga actggtaacc acaactcttt aaatgtaacg
     7561 ccgaataatc tacctgcatc tttaaagtta acctttttag acattaaaat tgtaataata
     7621 atagttaata aaatagctgt acctgtttgt ccaataatat ttaaagtcaa tactaatggt
     7681 ttatgcgtaa cttcgctgat tgttccaggt aagttgaatt taaatactaa tgatgataaa
     7741 gcaccatttg gtaagaataa gtttttaaag aatggcgcac tccaaatcat tacaatgact
     7801 gttaatacaa tgaatgggct ccatgcatgt aatactgctt ttgcagaatg tgcttttgca
     7861 ggttcaattt cttcatcttt attaacacga taaatgtgtt ttggttggaa ttttttagaa
     7921 aatactgcta atgctaacat tgttaataac ggtggaataa tatctgctaa ttcaggtcca
     7981 ctgaatacag ttaataatcc ttgagtaaat gtataagtga ttgaaactac taaaattgct
     8041 ggtaatgttt ctttaacacc tctaaaacca tcaataataa agataagtaa gaatggaata
     8101 atgaaattta tgattgccaa tgttaaagtt gctgattgag aaacacctaa tactgaaaca
     8161 tctccaggta atttcaacgt ttctacaaca cctacaggga taccaatcgc accaaaagca
     8221 ccagacgctg catttgcgac taaacataac atcgcagctt ttaatggatt aaatcctaat
     8281 tgtgttaata aaagtgcaca aattgcaatt ggtacaccaa atcctgctgc accttctaaa
     8341 aatgcgttga atgcaaatcc aataagtaaa acttgaatac gttggtcttg tgaaatattt
     8401 gtaatactat cttgaattgt taaaaattgt ccagattcaa cagtaatttt gtataacaat
     8461 actgccatca taacgatata gccaatcgga atgatacctt ggaagaaacc ttcgactact
     8521 gcaccagaag cgataccaac tggcaatttg aaaaatggta ttgcaattag taatgttaca
     8581 acaagtgttg taatagctgc ataaatacct ttcattttaa aaacagttaa acatagtaaa
     8641 aacagtacaa tcggtattgc tgcaattaaa gacgataata ataaattatc aaatgggtta
     8701 aacgtattta ctaacatagg ctgactcttc tttccataaa gtagtagtgt aattgcttca
     8761 ttaaaatcat gctctggcta tagcattttg ttatcgataa cattttacag aacagtgaga
     8821 ttaatgtgat ttttttcaca attaaaatat accatttttt cagactatta acaatatcac
     8881 tttcctattt aattgtttta tttttcattt taaatctgtt catcaaaatg aattgtactt
     8941 aatgtaatga agtaacttat actattcatg aattttacat aagaccgtgc taaaataagg
     9001 aagatactac gatgaggaat ggaagttata gcaatgatta aacagataaa tgtttctaat
     9061 atgcaaaagt ttgaatctca attaatgaaa gctcagtctg aaggttatac acatgtcgtt
     9121 ccatatgcca atgagataat gatttatcaa tcgatgttag atgcggttca attatatcct
     9181 aagtctatcg tggttgatta tactgtggat ggacaataca aaaatgattg tcactatttc
     9241 ggtcaatcat cgatcaacat tgccgattgg gctcaaaaca ataattatta tcctaacctg
     9301 atatatgcga ttcaacagac tcttgattta atacattatt actctgtaga aacgattttc
     9361 gatttagctt tattgacact tctgaaaggt gatttatcaa tcgatggtca tgtcgttttt
     9421 gattttaaag cccctatagc aacaagtgct tcaatatggg aaactattaa aactattgaa
     9481 gattttgata tgatgtctca gttttatttg aataaaatgg cttatattga tcatcatcct
     9541 ataccttttc gcaacctttt tatcgaggat tcagaacaac ttaattcgcc agataattgg
     9601 ttgtattcta ccaagtttat gctacctaaa tggttatata aaatagcaaa gcaacgtgct
     9661 gataacaaac aattacaaaa cttcggcctg tatacgaagc aaccaaacgt tttaaaagat
     9721 cacatcgttt ttatcggtga tcatcatcaa tatataggta attcaaaata tttatttact
     9781 tacttcgtta aacataatcc aatgactgcc tgttattttg ttactgacga tcgtcgcgga
     9841 ccacatttca tatcacctaa gtctgaaaaa gctgacgaat tgattaacag cgcacgcgta
     9901 gttatagttg aaaatgatat acctgaaacg ttacaaccaa acggtacttt aattcaatta
     9961 catcaaggca caccaataat gcaattattt ttagatagta aagagcctat taaaaatata
    10021 gaaacacctt tttatcgtgc taaaaggtat aatcgctggt tgcaattcga ttatgttata
    10081 cattctgctg atgatattag ccacttttat caaacggctt ttcctagtca ccaagcaaat
    10141 gtattggctt atggtaatcc taaacaccaa tatttactac aaaagagtaa cgaaagtact
    10201 actcaacaac agtataagaa atcgtttaaa ataaatgatc aaaaaccagt tttattatac
    10261 gcacctattg gattagtgag tgcgcaacaa ttaccactat cagatgcttt attcaaagcc
    10321 tatcatgttg tggttcaggg tgttgatgaa gcgatcttac cagaagaggc gcttgttgca
    10381 ccaaagtatc ttagtgctca agacttaatt ctaatgtctg atgtcgttat cactgattac
    10441 tcaaatataa tatttgatgc gatggctata gacaaaacag tagcattata cactccaaat
    10501 cacagtcaat acattgagtc tcaaggtgtt aacgaagata tttggcgtca tctatcaaaa
    10561 atttggtata cagaccgaca attattaatt aataatttaa tttcccaggc aataccagtg
    10621 attaaatatc cacaaataca acataaagaa caaccattag aatctatttc acaacttata
    10681 ttgtctaaga tgacatctaa tcaatagtgt ttaaatttct cagtggctgt gaatgaggtt
    10741 taaaagtact ataaaacgta aactttgata ctttaaaata cgcaaaaaac ggtaaaccct
    10801 aattcatatt atagagttta ccgttttatt ttttaacttg catcatagtt atattaacat
    10861 tattgttggt agtttggatc agtaaccatt gcttgtccag tataatcaac cgttacaatt
    10921 gaatattttc catttgcatt tgggtcttta aaactaaaca catacttata gttgccatta
    10981 tgttcttcaa tagaataatc attatacact ttattattac taccaaattt atttgcttca
    11041 ttattagccg catttaaagc tgtttggaaa tttggcaatt gctgtaaagc ttgattttta
    11101 tttccattaa acggataaat ttgacgtgca accggcgcgg cattttgtcc ataatatggt
    11161 gcaacgtaac ttgatttttg attattattc gcttggttat tacttgattg gttattattt
    11221 gtttggtttt ggtcattgtt tgttgcattt gaattagatt gttgctggtt atcgtttgca
    11281 ctattatctt tattatcttt gtttacgtct ttactatcat ctttattatc tttcttatct
    11341 ttagatgaat catttgtttt tttatcttgt tgttcagttt tcgctttatc atctttttct
    11401 ttattaccgt ctttttgttg gtcactatct tgaccacatg cagctaaaaa taatgataat
    11461 gctagtaacc ctgtaactaa tcttttcata catatctcct cctataattc gatattcatt
    11521 gaataatctt gaaatacata tctaccatgt gtatcttttc atggcttgtt accaaagacg
    11581 tcaacattaa tatgatctga attatcacaa tatccataac tacaatctaa ggttcttttt
    11641 attataccct aatttttgtt cattattatt taatttttgt gaattttatg ttttctataa
    11701 atttaattat tttactttaa caattcatta cgcatttagc atttcaaggt atacacaata
    11761 tttattacta tgatttcatt ttatctgctg caaaaacaat cattataact ctttttccat
    11821 aattaaatct gtatccgtta catcacctgt ttgaaaatga tgttcaccaa ccactttaaa
    11881 tccatgacgt ttataaaatg cttgagcacg aggattatgc tcccaaactc ctagccaaat
    11941 tttatgttta ttatgttctt gagcaatttt ttcggccaat tctatcaatt gtgaacctct
    12001 tccgccacct tgaaagtctt tcaaaaaata tatgcgctgc acttctaaat aggtctcccc
    12061 catttcttca gtttgagcac tattaatatt catctttata taaccaacat tcgcaccatc
    12121 ttcttgataa aaataatgaa atgaatctac atggttaatc tcttgtgtaa atttctctac
    12181 agtataattg tctttaaaaa attgatcaaa atctttgtca tcatagtaag aaccaaacgt
    12241 gtcataaaat gttctagttg ctaattcaac taattcacta gcattttgtt ctgaaatttc
    12301 tttgattatc ccagccatat aaatcctcca ataaacagtg atcgaatcaa aatattactt
    12361 atgttatttt tcagccaaaa ctatttaaaa atacattaac acaaatcaat tacaaattgt
    12421 attgattgtg tgtaacatca ataaatgata catttattcc agtaaaatgg ccgtattttc
    12481 aaaagagaaa aagagaggat gtatcgttgt gatagaaaca tttaaagcgt ttgtaattga
    12541 taaagatgag agtggtaaag tgacaccaac tttcaaacaa ttatcgccta ctgatttacc
    12601 taaaggagat gtgctgatta aagtacatta ctctggtata aattataaag atgctttagc
    12661 gactcaagat cataatgcag tcgtaaaatc gtatcctatg attccaggaa tagatttagc
    12721 tggaacaatt gttgaatccg aagcaccagg ctttgaaaaa ggagaacaag taattgtaac
    12781 gagttatgac ctaggtgtca gccattatgg cggttttagt gaatatgcgc gtgtaaaatc
    12841 agaatggatt atcaagcttc ctgatacttt aacattagaa gaatcaatga tatatggcac
    12901 agctggttat actgccggtt tagcaattga aagacttgaa aaagttggaa tgaatattga
    12961 agatggtcct gtactcgttc gcggtgcttc aggtggtgtc ggtactttag cagtactcat
    13021 gcttaatgaa cttggttata aagttatcgc aagtacaggt aaacaagatg ttagcgatca
    13081 attacttgaa cttggtgcca aagaagttat cgatcgactt cctgttgaag atgatcataa
    13141 aaagccactc gcatcatcaa cttggcaagc ttgtgtagac cctgttggtg gcgaaggtat
    13201 taattatgtt acaaagcgtt taaatcatag tgggtcaatt gcagttattg gtatgactgc
    13261 cggtaatact tatactaatt ctgtattccc tcacatttta agaggtgtaa acattttagg
    13321 aattgactcg gtatttactg ctatgaaatt aagacagcgc gtttggcgtc gtctcgcaaa
    13381 agatttaatg cctgaaaatt tacatgagat caagcaagtt attacatttg atgaacttcc
    13441 agaacaactt aacaaagtaa ttaaacatga aaataaaggg cgcattgtta tcgatttcgg
    13501 tgtagataaa tagtattcat gaaaaagaca tcccgttatg cgagatgtct tttttaattt
    13561 agtatttgat atacataccg cctgaatctg gttcggtagg tataaatcca aattttgtat
    13621 ataatttatc cgctgggtag tctgcaatca gactaacgta tgtactctca acagccacac
    13681 ctttaatata ttgcataata tgctccataa ttagactgcc gtaaccttga ccttggtaac
    13741 ttttcaaaac tgcaatatca acaatttgaa aaacagttcc gccatcgcca atcactctac
    13801 ccataccaat taaccgatct ttatcataca aggttactgt aaataaggca ttaggtaatc
    13861 ctttttcagc tgcttcgcgc gtctttggac tcatacctgc gttaatcctt aatgcgcaat
    13921 aatcctcgca agtcggaata tcatatgtca ctttaaccat tatttacccc acttttcatc
    13981 acacaatata tcaacctagt ataaatgttt atttacaata gtcttattcg cttctttaaa
    14041 cacttcatga tgacttgaaa cataaccctc tgcattcgca tctggttgga tatatgtttt
    14101 agcaaggttc gctgcatttg caccatcact aaatgcactt gcaattagat gtgattttgc
    14161 atcatgataa acaatatctc cacacgcata gataccaggt atactagttg tcgtattacc
    14221 aaatccttta acacgacaat catcatgcat atctagcttt gaagatgttt cactcaataa
    14281 tgtattacaa cgatcaaacc catgactaat aatgacatcg tcaaatttaa ctgtatgcct
    14341 atcgccactt tcaacatgtt ccaaaacaac ttcacttata tgcgtttcat catcattgcc
    14401 gaccaagtat ttaatacgtg tttttgggca tagtttcaca tttaaatctg tcaccaacgt
    14461 tttcatcgct tcatgaccac ttacatcttc ttttcgataa acaactgtca cgcttttagc
    14521 aatcttggca atatcatgcg cccaatctaa tgctgtattt cctccacctg atattaatac
    14581 atctttatct ttgaaacgtc tgtaactttg tacaacataa tgtaaattag ttaattgata
    14641 tctctctaca cctttaacat ctaattgttt tggattaata atacccgcac caattgcaat
    14701 gataactgct ttcgatgtat atatttctcc cgcttctgtt tcaacttcga aatgacgttc
    14761 tgcctttttc ctaatatcta ccacacgttc attcaaatga acttccggtt taaaatataa
    14821 tccttgctta attgtatctt ttaaaatttc atgacaaggt tttggcgcaa tgccgccaat
    14881 atcccaaata attttttcag ggtaaattct catcttaccc cctaattcag attgaacatc
    14941 tatcaatctt acagacatat ctcgcaatcc agcataaaag cttgcataca aaccagacgg
    15001 accgccacca atgattgtaa catctttcat tatgtgcctc ctatgactct ctatattcat
    15061 ttctttcatt aacgtgctca aattgataat tattatcatt taaagccatt atactattaa
    15121 tatttatatt gttaaaataa atcgcatagt tagccatgaa ttatcaataa ctatcccatc
    15181 acaatataaa aacacacctt tcaaagtaag aaacaacaat gttcctttga aagatgtgta
    15241 tattttttag ttctagttat attatttttt aaaagactca tcacgtggtt ctttaagaat
    15301 tgcttgtctt aaaaggaaaa atagcaacaa taaacctgca agcatacctg tgtgcccaat
    15361 acctgcaaag cctgcaaatg cttctggaga gtatgattta ccagtgactt ggaagaatcc
    15421 ttttgtcacc atcataccta tagtaacgat aacacctatg ttatatacat agaagaacca
    15481 gttaaataag tagtaacttg ataatttaaa taacttctcc aatggtaata atattaaaaa
    15541 cataaacata cctaaaataa gtgtatgtgt gtgtacaagt gataattgtg ttgtaccagt
    15601 aaaatcatat gcttttgtta gctctctata gaaaaagcca ctaaataaac ctatgatcat
    15661 ataaaaaaga aatgaatata ataacctacg cattttgaat cctccctcac cttttcatta
    15721 tatccgatta tacctattat aacagtgaca aaaaagcgaa gatatgacac aactgaattt
    15781 ggtttagcac atagtatgac agctaaaata actttacatc aagcgctata caaataaaat
    15841 agctatacca tgacgctcgc tacaaacgtc ttgtatagct attatttcta tttattaaat
    15901 tatgacggca cttcagtacg ttttgtgcta tgttttctaa caatagctga aactaaactt
    15961 gatacagcgc caaaaatcat aaaccctata aacatccaca ttgtactatt taattcaata
    16021 tgatgattca agtataatag ttctcttaca cttgttgcat agtgtgtgaa tggattccaa
    16081 tcatatacat atttttgata actttcaggc aacatttgtt taggtaacgt tacaagttgc
    16141 atactaaaga acattaaaat gaagaaaatt ggaactgact tcatacctag ccataccata
    16201 acacctaaaa taaggcccac gaaaccaaga atcgcaaatg ctacaaaaat tgcgatacga
    16261 tttggatggt caaaatcaaa tctttgaacg ccttgcatga aataaatata aacaaagcta
    16321 cctgcaaatg ctgcaacaac tgcaaatatc atctgtccaa ttgaagcaat gatacgatgt
    16381 tgcacgacga tattgttact agttctaaat gcaaagaaca ataagataga cgttacgatt
    16441 gaacctatcc aaattggcat aaacattagg aatggtgcat taccacctgc ttggtgatct
    16501 ttaactttat taagtttttc attatccact tttactggat tcgtcaaacc attgatgtcc
    16561 gcggcattga ctttaacatt ttgactcgtt aatgtttcca aactttgctt cgtaatttga
    16621 gcattaatat tatcacccat accagttaac acagctgatg caatttgtga accttgtaag
    16681 cttgatcctt cacttacaat cgttttaaat ttagcctgct taacctctac ttgttgattc
    16741 cccatttttt gtttcatttg ttgaaccact tgtggcggaa tttcacctga agcaactttt
    16801 tgttgcattt cttctttttt actatccata actacttttt gtgttttact catagcatct
    16861 tttgaaaagt ctttatcaat aatagctact ccaaagattt tttgatcttt caaatctttt
    16921 tcaaggtctt tttcactatc aaccttaacc catttaattt tattagaatc actatctaat
    16981 aatttatcct ctaatttttt accaatgtta acatttttat cttgaatcgt tgtaccttta
    17041 tcctcgttta atataccaat tggtaaatct tttggtttag gattatatgc aggataaaaa
    17101 gctaaagaaa agattaccaa gataatcata gttgctattg gtgcaatcca taataattta
    17161 tttttaaata tattcatttt attaattacc tccttattag acaatgtgtt gaatatttcg
    17221 catgttgtct attataatat gtaagtggga ataaacaata cacaatgttc atcaatacgt
    17281 tcattaatag acaaatacac ccacattgtt caataacaca aggaggttga tttatttttg
    17341 aaagaagata ggcgaattag aaagacaaag tcctccatta aacaagcgtt cactaaatta
    17401 ctacaagaaa aagatttaga aaagattacc attcgcgaca taacaacacg cgctgatatc
    17461 aatagaggga cattttactt acattacgag gataaatata tgttactcgc agatatggaa
    17521 gatgagtata tttcagaact aacgacatat actcaatttg atttgttacg cggttcttca
    17581 attgaagaca ttgcgaatac ttttgtaaat aatatactca aaaatatttt ccaacatatt
    17641 catgataatt tagagtttta tcatactatt ttacaattgg aacgcacgag tcaattagaa
    17701 ttgaaaatca acgaacatat taaaaataat atgcaacgtt acattagtat caatcattct
    17761 atcggaggcg ttccagagat gtatttttac agttacgttt ccggagcaac aatttcaatt
    17821 attaaatact gggtaatgga caaacagccc atttcagttg atgaattagc taaacatgtg
    17881 cataatatta tttttaatgg tccattaaga ataatggcag aaaaccgttt gcataaatca
    17941 aaccttgatt cactaactta agtaatactc gcttttccta aatcctttag aaaggaacta
    18001 tatatcatga ttacttcatt cagacattct gaagatatag ataaacatat tataaaaaca
    18061 ccattagatc atacagcgtc atggattaat gtagtagaac cagaccgaga agaaattgaa
    18121 aatcttatgg aacaatataa tatacctgaa gactttatac gtgacccttt ggactcagaa
    18181 gaaagttccc gtattgaata tgacgaagat actggttact cattaatcat tattgattta
    18241 cctatcgtca attcaactaa tcgtagcgtt ctatcttttg taacgattcc attaggcatt
    18301 attattggca acggtattat cgtaacagtt tgtgacgctg aaaatgaatt tttagaaaat
    18361 ttacctaagc gtgatattaa tttaaaattt cacagcagat ttgcattaga aattttaact
    18421 actattgccg accattataa tcgtaactta cgattactta ataaaagtag aattcgtatt
    18481 gagaaagaac taaaaaataa cattactaac aaacaacttt tcaaattaat ggaagttgaa
    18541 aaaagtttag tatacttttt agctgcctta aaaggtaacg atacaattat taaaaagtta
    18601 ttccgtttac ctgcaattaa acgttttgaa gaagatgaag aattactaga agatttaatc
    18661 attgaaaata accaagccat cgaaacaaca gaattacatc aacgtatctt agaaagtatc
    18721 acaacatcat acgcttcctt attatctaat gatatgaata cgattatgaa gacattaaca
    18781 cttttcacgg tactattaac gttaccaaca ctcgtattta gtttcttcgg tatgaatgtg
    18841 ccgttgccaa ttgatgacca tagttatata tcttggatta ttgtggtagg tatttcatta
    18901 attcttgtcg taattgttag tattttctta tggcgtaaac agaaattata actaaattaa
    18961 aaggtatgac agcttaaagt ttcatcctat tgcatttgca atgactgata ctttgattac
    19021 tgtcatacct ctttttttat tctacaactt ctttatttgc ttttctttta gataaaatta
    19081 aagtaactgt tatagcaatg atgaatgata ttgtcatacc aacaaagtag tgtaaccatc
    19141 ctgcatgtac tggattgatt gaaataaatc ctggcaatcc agcagttcct aatgcgattg
    19201 ctttaacctt gaagaaagca atatatgctg aaccaatacc tgatccaacg atagcgccaa
    19261 taaatggata tcttagtttt aagttaacac caaacatagc cggttctgta ataccaagta
    19321 atgctgaaat acctgcggca gatgccacac cttttaactt cttattttgt ttaataataa
    19381 agaacgctgc aattgctgca ccaccttgtg caacattaga cattgtcgca atcgggaata
    19441 taaatgatcc acccgtttta gtggcatctg caattaatgt cgtttctaca gctataaagc
    19501 tatgatgcat acctgtaata acaatcggag catacaataa tccgaatatt aatccaccaa
    19561 ttgcaccacc aaattcataa agccatgtta aaccatctga taaccagtaa cctaattgtc
    19621 gagtgattgg acctacaaat gaaaatgtta gaaatgctgt tataaaaata gataacaatg
    19681 gtgttaacaa attatctaac accgttggaa taactttgcg taaccctttt tcaattgagg
    19741 ctaagatata agctgctaca agcataggta acacttgtcc ttgataacct acttgattaa
    19801 tatgcaaacc aaaaacatcc caatatggaa tagccttacc tgcttcaact gcttttggga
    19861 aatcgtatgc gctcattagc gatggatgaa ctagtatcat acctaatgca gcacctaaaa
    19921 atggattgcc accaaagcgt tttgctgcac taaatccaat taaaattggt aataatgtaa
    19981 atggtgcatt cgcaaaaaca tttatcattt cagctaatcc agcaaattga ctatatacat
    20041 ctatcaatga tttacctgaa aagaataaat ctttcgcagt caaaatgtta tttaacccca
    20101 ttaataaacc accagcaacg atggctggta taatcggaac aaagatatct gaaagcattt
    20161 ttacaaatcg ctgtaacgga ttcatatttt tagcagattg tgctttgact tccgaagtgg
    20221 ttgatgcttc ttttccagtt aatttttcca gttcactaaa tactttattg actgtaccag
    20281 acccaataat aatttggtat tgtcccccag tagaaaacgt ccctttaact acatccatat
    20341 tgtttagcgc ctcttcattt actaaacttt catcatttaa aactaatcgt agtctcgttg
    20401 cacaatgcgc cattgcatct aaattctctt ctccgcctat cgcgttcaaa atttcttctg
    20461 cggattgctt ataattcata tatttagcct cctaattaga accggttcca tttattccac
    20521 tttattgtaa ccggttccaa aaatatttgc aacactctat tatcatttta tataaacacc
    20581 tacaccattt ttgatagcgc attcattttt attgctatac tacatgcatg tatattaaca
    20641 ttaattatcg gaggtaattt tatgaaagaa cgtggctata tcgaacagct ttggcgtgag
    20701 gaaaaatatc atgtattgtt acacagtcaa cagtcttacc aaatgataag gaatgcttta
    20761 aaaacggatt tatcacttca tcaagttcaa caaatgattg atgttgcttt gttaattgaa
    20821 ccgagtatag gaagtgtatg taatgctttt gatcatatgt ggggatattt aaaaaaatgt
    20881 gcgaatgaag aagaaagaca acaatcaaaa ctacttaaag ctgattttat taatggtaaa
    20941 atcgacaccc aaactttatt agatttttta gcagaacttg ctaataaata tgatgttcaa
    21001 tatttactac aaagtcgtgt tttaaacaca aaaagaaaga gatagattga aactttagtt
    21061 gtaacggaca aatagtagtc attggagttc agcaattcat actttgtact aaagtttact
    21121 ataatctatc tctcaaggtt taaatgtaca tatctatatt aaaatgcgtt tcatgatgtt
    21181 agaagatacc atacacattt aattagttac agcataaatc aatttatacc cttaattatt
    21241 acattgttta tattttaaat tcgggggagt aattttaagt aatatcttgt tgctgctaat
    21301 tctattgtta ataaattgat attagatgta gaaattgagt gtgaaagtta ataatagatg
    21361 atttagctag taacataatt aattcatata tagtcaaaaa gttctgttag atactgttct
    21421 tgatgaatat tctttgatgt taggtgttag ctattctaga tgagtttctt agatgtggat
    21481 gcctgtcttg atgaagttat ctatgatgtt aatataatac ttgatgttga ttcttagatg
    21541 ttagtgcgat ttcaagatgc tgattctttg ttgttgcact atcttggtga cgtttctagt
    21601 tgtttatagt tatcgcatct cttgatgtaa aatctaagat gtttatgcat ttctagatgt
    21661 taattcttcg ctgcttaaga attatcttga tggtaaatct agttgtgcca tgcattttct
    21721 catgatgaat ctaaggtgtt aattcattga tgtgtgctgt ttcttgttgc ttcattatct
    21781 tgatggtgaa tttcgttgtc taatgcactt tttcaaatga ttattctaat tgttacagca
    21841 ataactagat ggattttctt agatgttcac ttttctagat gtttatactt atcttgatgt
    21901 tttctaaaca gaacttttaa acgctttatt ttttagtaga ttgtttaaaa tttcttagtt
    21961 gatggttatt gttgttgatt tgaataattt gtagcgaata ttttgtaaca gggctactaa
    22021 gaatttttat aacaagaagt gatttcaatc gtactaattt tcagcgtttg atgacaaaag
    22081 tgtatgtcca aacttaaata atggcgataa aatttaagtc atgggtacat ttaatgtttt
    22141 ttgattcaaa cttccatttt ggtcaatttg ctttttttat aacatagttg aatatcaata
    22201 aaagattagc aagttagttc aaaatggcta tgtattgttt tttgaaatac ataggtttga
    22261 aatctatatt aattatgcac caaataattt aattagactc tatcgaaaat ttccaaattt
    22321 tgcttactat ctttctaatt ttacttttat attgttcgca atgcataaaa acaagcgttt
    22381 tcctaggagt tttcattaat ttcttttatt tttcactatt accttttcaa aaat
//
LOCUS       DALAGD010000048        22063 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.048, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000048 DALAGD010000000
VERSION     DALAGD010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22063
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.048"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            132..479
                     /gene="sarR"
                     /locus_tag="O2701_001442"
     CDS             132..479
                     /gene="sarR"
                     /locus_tag="O2701_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarR"
                     /protein_id="HCZ4293341.1"
                     /translation="MSKINDINDLVNATFQVKKFFRDTKKKFNLNYEEIYILNHILRS
                     ESNEISSKEIAKCSEFKPYYLTKALQKLKDLKLLSKKRSLQDERTVIVYVTDTQKANI
                     QKLISELEEYIKN"
     gene            complement(668..1504)
                     /locus_tag="O2701_001443"
     CDS             complement(668..1504)
                     /locus_tag="O2701_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HCZ4293342.1"
                     /translation="MDEQQWTGQLDLTVFFDGNRSVSRDIFFEKALKVIRPVYLNQST
                     IPTFYIVNVGGGYLDGDRYRMNVNVEDNAKVTLTSQGATKIYKTPSNHVEQYQTFNLK
                     DNAYLEYVADPIIAYENAKFYQHNTFNLNNSSSLFYTDILTPGYSKTGEAFKYQYMHL
                     INEIYIEDELVTYDNLLLNPNKQSINEIGYMEHYSHYGSAYFIHEDVNQKLIDSVYET
                     ISSYSNTFDCRVAISQLPTHGFAVRIFAYRTQIIEKILGTIQSYIAENIYDRKLDFLR
                     KY"
     gene            complement(1504..2118)
                     /gene="ureG"
                     /locus_tag="O2701_001444"
     CDS             complement(1504..2118)
                     /gene="ureG"
                     /locus_tag="O2701_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HCZ4293343.1"
                     /translation="MANPIKIGIGGPVGAGKTQLIEKVVKRLSKEMSIGVITNDIYTK
                     EDEKILVNSGVLPESRIIGVETGGCPHTAIREDASMNFAAIDELLERHDDIELIFIES
                     GGDNLAATFSPELVDFSIYIIDVAQGEKIPRKGGQGMIKSDFFVINKTDLAPYVGASL
                     EQMAEDTKVFRGKRPFTFTNLKTDEGLDEVIDWIERDTLLKGLS"
     gene            complement(2131..2820)
                     /locus_tag="O2701_001445"
     CDS             complement(2131..2820)
                     /locus_tag="O2701_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HCZ4293344.1"
                     /translation="MIDHTHLRLFQFCDSQFPTGAFSHSFGLETYIQRNIIHDDHTFI
                     AWLKMFLQEQLTYSDGLAMRLVYDALENDDTQKVLHIDKLMFVQNLPKETRVGAKQMG
                     TRMVKLALELYNSPWIAWYHQQMQDKKAKLNPAICFTMLGHHLGVDIETIIDYYLYQN
                     VSSLTQNAVRAIPLGQTAGQKIVTHMIPYIEGTRKQIFELKEADFGMTAPGLELNQMA
                     HENVNVRIFIS"
     gene            complement(2813..3265)
                     /gene="ureE"
                     /locus_tag="O2701_001446"
     CDS             complement(2813..3265)
                     /gene="ureE"
                     /locus_tag="O2701_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HCZ4293345.1"
                     /translation="MIVEEIQGNIANLSNSEKQKHVEKVYLENSDLVKRIQRVVTDHG
                     TEIGIRLKQPIDLQYGDILYADDHNMIIVDVNSEDLLVIQPRTLQEMGDIAHQLGNRH
                     LPAQFTETEMLVQYDYLVEDLLKSLGIPYVREDRKVNKAFRHIGHSHD"
     gene            complement(3278..4993)
                     /gene="ureC"
                     /locus_tag="O2701_001447"
     CDS             complement(3278..4993)
                     /gene="ureC"
                     /locus_tag="O2701_001447"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HCZ4293346.1"
                     /translation="MSFKMTQNQYTSLYGPTVGDSIRLGDTNLFAQIEKDYAVYGEEA
                     TFGGGKSIRDGMAQNPRVTRDDVNVADLVISNAVIIDYDKVVKADIGIKNGYIFAIGN
                     AGNPDIMDNVDIIIGSTTDIIAAEGKIVTAGGIDTHVHFINPEQAEVALESGITTHIG
                     GGTGASEGSKATTVTPGPWHIHRMLEAAEGLPINVGFTGKGQATNPTALIEQINAGAI
                     GLKVHEDWGATPSALSHALDVADEFDVQIALHADTLNEAGFMEDTMAAVKDRVLHMYH
                     TEGAGGGHAPDLIKSAAFSNILPSSTNPTLPYTHNTVDEHLDMVMITHHLNAAIPEDI
                     AFADSRIRKETIAAEDVLQDMGVFSMISSDSQAMGRVGEVITRTWQVAHRMKEQRGPL
                     DGDFEHNDNNRIKRYIAKYTINPAITHGISEYVGSIEPGKLADIVLWDPIFFGVKPEL
                     VVKGGLINSAVNGDANGSIPTSEPMKYRKMYGQYGGNLTSTSMTFVSKTAYENGINRA
                     LNLKRMVRPVKNIRQLSKADMKNNSATPKLDVDPQTYEVYVDGEKITSNAATELPLTQ
                     RYFLF"
     gene            complement(4990..5400)
                     /locus_tag="O2701_001448"
     CDS             complement(4990..5400)
                     /locus_tag="O2701_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HCZ4293347.1"
                     /translation="MIPGEIITKSTEVEINNHHPETVIEVENTGDRPIQVGSHFHFYE
                     ANAALDFEREMAYGKHLDIPAGAAVRFEPGDKKEVQLVEYAGKRKIFGFRGMVNGPID
                     ESRVYRPTDENDEYAGVFGDNGAENVNKKGGKRS"
     gene            complement(5414..5716)
                     /locus_tag="O2701_001449"
     CDS             complement(5414..5716)
                     /locus_tag="O2701_001449"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HCZ4293348.1"
                     /translation="MHFTQREQDKLMIVVAAEVARRRKARGLKLNHPEALALISDELL
                     EGARDGKTVAELMSYGRQILNKEDVMDGVEHMITDIEIEATFPDGTKLITVHHPIV"
     gene            5976..6881
                     /gene="yut"
                     /locus_tag="O2701_001450"
     CDS             5976..6881
                     /gene="yut"
                     /locus_tag="O2701_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HCZ4293349.1"
                     /translation="MKIIDVLLKNISQVVLISNKWTGLFILIGLFVADWTIGLAAIVG
                     SIIAYTFARFINYSEAEINDGLAGFNPVLTAIALTIFLDKSGLDIVITMIATLLTLPV
                     AAAVREVLRPYKVPMLTMPFVIVTWFTILLSGQVKFVDTSLKLMPQNIETVNFSNNDR
                     IHFIQSLFEGFSQVFIEASVIGGVCILIGILIASRKATLLAVIASLLSFIIVALLGGN
                     YDDINQGLFGYNFVLMAIALGYTFKTAINPYISTFLGVLLTVVVQLGTTTLLEPFGLP
                     ALTLPFIIVTWILLFAGIKHDKVDA"
     gene            7098..8252
                     /locus_tag="O2701_001451"
     CDS             7098..8252
                     /locus_tag="O2701_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HCZ4293350.1"
                     /translation="MNDMDNSFLITTEIQRKWIEKFKVIRDTFKAKAEYNDQHSQFPY
                     KNIEWLIKEGYGKLTLPKAYGGEGATIEDMVILQSFLGELDGATALSIGWHVSVVGQI
                     YEQKLWSQDMLEQFAVEINNGALVNRAVSEAEMGSPTRGGRPSTHAVKADDGYILNGV
                     KTYTSMSKALTHIIVAAYIEELESVGFFLVDRNLPGVEIADNWDVLGMRATESHDLIL
                     NDVKVPLKHLVETEKSKAPNGWILHIPSCYLGIAQAARNYAVDFAIQHSPNSIEGTIA
                     TLPTVQQNLGKMETLLLCARQFLWSTAKGYQQYKDDSQIRNPTSASKVMVMNQGLEVI
                     DLAMRIVGAKSLEMNRPLQRYYRDMRAGLHNPPMEDAAYTNIAKSITDTF"
     gene            8466..9374
                     /locus_tag="O2701_001452"
     CDS             8466..9374
                     /locus_tag="O2701_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCZ4293351.1"
                     /translation="MKKLLLPLIIMLLVLAACGNQGEKNNKAETKSYKMDDGKTVDIP
                     KDPKRIAVVAPTYAGGLKKLGANIVAVNQQVDQSKVLKDKFKGVTKIGDGDVEKVAKE
                     KPDLIIVYSTDKDIKKYQKVAPTVVVDYNKHKYLEQQEMLGKIVGKEDKVKAWKKDWE
                     ETTAKDGKEIKKAIGQDATVSLFDEFDKKLYTYGDNWGRGGEVLYQAFGLKMQPEQQK
                     LTAKAGWAEVKQEEIEKYAGDYIVSTSEGKPTPGYESTNMWKNLKATKEGHIVKVDAG
                     TYWYNDPYTLDFMRKDLKEKLIKAAK"
     gene            9773..10714
                     /locus_tag="O2701_001453"
     CDS             9773..10714
                     /locus_tag="O2701_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCZ4293352.1"
                     /translation="MTKKVYFNHDGGVDDLVSLFLLLQMENVQLIGVSTIGADCYLEP
                     SLSASVKIINRFSNEDIQVAPSYERGKNPFPKEWRMHAFFMDALPILNEPVKHVASNV
                     SDKEAFEDIIQTLKRQSEKVTLLFTGPLTDLAKALQKDSSIVQYIEKLVWMGGTFLPK
                     GNVEEPEHDGSAEWNAYWDPEAVKIVFDSDIEIDMVALESTNQVPLTLDVRQRWANER
                     QYTGIDFLGVSYAAVPPLTHFITNSTYFLWDVLTTAYIGNKDLVHSIEKKVDVISYGP
                     SQGKTFECKDGRKINVINHVDNNAFFDYITALAKKVN"
     gene            10835..11389
                     /locus_tag="O2701_001454"
     CDS             10835..11389
                     /locus_tag="O2701_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HCZ4293353.1"
                     /translation="MTTKQLVYTALMTAIIAILGLVPVIPLPFSSVPIVLQNIGIFLA
                     GVILGRKYGTLSVIVFLLLVVAGLPLLSGGRGGIGVFAGPSAGFLLLYPVVAFMIGAI
                     RDRFINEINFWILFVGILVFGVIALDVIGTLIMGMIINIPFTKAISISLAYLPGDILK
                     AIVASLIGTALLNHSQFRQIMGIK"
     gene            11461..12231
                     /locus_tag="O2701_001455"
     CDS             11461..12231
                     /locus_tag="O2701_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4293354.1"
                     /translation="MASISMSDIYCNGTIFENDDEQLIYLTPSFPQRYTSNTWIYKKT
                     PTQERWLKDLERQHQLHTNQGSNHYAFSFPENEQLDNHWMAMFKDMNFELGIMELYAI
                     ESDALANLPRNSDVEIAIVDESHIDAYLKVAYQFSLPFGKDYADAHEEMVREHYQKDV
                     IKRLVAYLNNEPIGVVDVIESENYIELDGFGVLEQFRHQGIGSTIQSLIGEYAISKNH
                     KPIILVADGEDTAKDMYAKQGYVYQSFCYQILKEDIGN"
     gene            complement(12403..13200)
                     /gene="fdhD"
                     /locus_tag="O2701_001456"
     CDS             complement(12403..13200)
                     /gene="fdhD"
                     /locus_tag="O2701_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCZ4293355.1"
                     /translation="MNKDVSLGQPIVRYEDGKLFNTTDQYVTEFPLTIMVNGEEFATV
                     ICSPTNLEELVIGFLASEGAILKRDELKSVLIDDSKGFAHVELNKDLGDRFQYSTKRM
                     IASCCGKSREFYFQNDAAIAKTSMSKITLTPMQIINMMTRLQSASHIYQETGGLHNAA
                     ISDGLTFFVHRQDIGRHNALDKLYGFCIQRHITVRDKVLIFSGRISSEILIKAAKIGV
                     GVILSKSAPTTLAVTLANDLNITAVGFIRNGGFNIYSHPERIIDSEQ"
     gene            13490..14272
                     /gene="modA"
                     /locus_tag="O2701_001457"
     CDS             13490..14272
                     /gene="modA"
                     /locus_tag="O2701_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCZ4293356.1"
                     /translation="MKLKRLFAVVIAMLLVLAGCSNSNDNNESKKDDADNGKKQEIQV
                     AAAASLTDVTKKLASEFKKEHKNADIKFNYGGSGALRKQIESGAPVDVFMSANTKDVD
                     ALKDKNKAHDTYKYAKNSLVLIGDKDSNYTSVKDLKDNDKLALGEVKTVPAGKYAKQY
                     LDNNNLFKEVESKIVYAKDVKQVLNYVEKGNAKQGFVYKTDLYKQNKKIDTVKVIKEV
                     ELKKPITYEAGATSDSKLAKEWMEFLKSDKAKEILKEYHFAA"
     gene            14286..14957
                     /gene="modB"
                     /locus_tag="O2701_001458"
     CDS             14286..14957
                     /gene="modB"
                     /locus_tag="O2701_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCZ4293357.1"
                     /translation="MPDLTPFWISIRVAVISTIIVTVLGIFISKWLYRRKGSWVKLLE
                     SLLILPIVLPPTVLGFILLIIFSPRGPIGQFFANVLHLPVVFTLTGAVIASVIVSFPL
                     MYQHTVQGFRGIDTKMINTARTMGASETKIFLKLILPLAKRSILAGIMMSFARALGEF
                     GATLMVAGYIPNKTNTLPLEIYFLVEQGRENEAWLWVLVLVAFSIVVISTINLLNKDK
                     YKEVD"
     gene            14958..15563
                     /locus_tag="O2701_001459"
     CDS             14958..15563
                     /locus_tag="O2701_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCZ4293358.1"
                     /translation="MLKINVKYQLKNTLIRINIDDTEPKIYAVRGPSGIGKTTVLNMI
                     AGLRKADEAIIEVNGQLLTDTAKNVNVKIQQRRIGYLFQDYQLFPNMTVYKNITFMAE
                     PSEHIDQLIQTLNIDHLMKQYPMTLSGGEAQRVALARALSTKPDLILLDEPFSSLDDT
                     TKDESITLVKRIFNEWQIPIIFVTHSNYEAEQMAHEIITIG"
     gene            15733..16737
                     /locus_tag="O2701_001460"
     CDS             15733..16737
                     /locus_tag="O2701_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HCZ4293359.1"
                     /translation="MSQERYSRQILFKQIGEIGQSKINQKCALIIGMGALGTHVAEGL
                     VRAGIAKLIIVDRDYIEFSNLQRQTLFTEEDALKMMPKVVAAKKHLLALRSDVDIDDY
                     IAHVDYYFLETHGQDVDVIIDATDNFETRQLINDFAYKYRIPWIYGGVVQSTYTEAAF
                     IPGKTPCFNCLVPQLPALNLTCDTVGVIQPAVTMATSLQLRDAMKVLTEQPIDTKITY
                     GDIWEGSHYSFGFSKMQRSDCTTCGDVPSYPYLNKNEQRYATLCGRDTVQYENASITH
                     DILVQFLKQHQLNYRSNSYMVMFEFKGHRIVAFKGGRFLIHGMTRTSDATHLMNLLFG
                     "
     gene            16768..17274
                     /locus_tag="O2701_001461"
     CDS             16768..17274
                     /locus_tag="O2701_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaB"
                     /protein_id="HCZ4293360.1"
                     /translation="MGEHQNVKLNRTVKAAVLTVSDTRDFDTDKGGQCVRQLLQADDV
                     EVSDAHYTIVKDEKVAITTQVKKWLEEDIDVIITTGGTGIAQRDVTIEAVKPLLTKEI
                     DGFGELFRYLSYVEDVGTRALLSRAVAGTVNNKLIFSIPGSTGAVKLALEKLIKPELN
                     HLIHELTK"
     gene            complement(17276..17770)
                     /gene="moaC"
                     /locus_tag="O2701_001462"
     CDS             complement(17276..17770)
                     /gene="moaC"
                     /locus_tag="O2701_001462"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCZ4293361.1"
                     /translation="MTEFTHINQQGHAKMVDVSDKQITKRTAVAHSSITVNETIFKQI
                     SNNTNTKGNVLNTAQIAGIMAAKNTSTLIPMCHPLPLTGIDVHFSWDETNAPLYTLNI
                     QTTVSTTGKTGVEMEALTAASATALTIYDMTKAVDKGMIIGETYLESKSGGKSGDFQR
                     QSNQ"
     gene            17833..19092
                     /locus_tag="O2701_001463"
     CDS             17833..19092
                     /locus_tag="O2701_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCZ4293362.1"
                     /translation="MVVEKRNPIPVKEAIQRIVNQQSSMPAITVALEKSLNHILAEDI
                     VATYDIPRFDKSPYDGFAIRSVDSQGASGQNRIEFKVIDHIGAGSVSDKLVGDHEAVR
                     IMTGAQIPNGADAVVMFEQTIELEDTFTIRKPFSKNENISLKGEETKTGDVVLKKGQV
                     INPGAIAVLATYGYAEVKVIKQPSVAVIATGSELLDVNDVLEDGKIRNSNGPMIRALA
                     EKLGLEVGIYKTQKDDLDSGIQVVKEAMEKHDIVITTGGVSVGDFDYLPEIYKAVKAE
                     VLFNKVAMRPGSVTTVAFVDGKYLFGLSGNPSACFTGFELFVKPAVKHMCGALEVFPQ
                     IIKATLMEDFTKANPFTRFIRAKATLTSAGATVVPSGFNKSGAVVAIAHANCMVMLPG
                     GSRGFKAGHTVDIILTESDAAEEELLL"
     gene            19089..19574
                     /gene="mobB"
                     /locus_tag="O2701_001464"
     CDS             19089..19574
                     /gene="mobB"
                     /locus_tag="O2701_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCZ4293363.1"
                     /translation="MILQIVGYKKSGKTTLMRHIVSFLKSHGYTVATIKHHGHGKEDI
                     QLQDSDVDHMKHFEAGADQSIVQGFQYQQTVTRVDNQNLTQIIEKSVTIDTNIVLVEG
                     FKNADFEKVVVYRNEEELQVLQQLSNVCYSINVREHEDFTAFEQWLLNKIKNDCDTQL
                     T"
     gene            19588..20034
                     /locus_tag="O2701_001465"
     CDS             19588..20034
                     /locus_tag="O2701_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HCZ4293364.1"
                     /translation="MKQFEIVTEPIQTEQYREFTINEYQGAVVVFTGHVREWTKGVKT
                     EYLEYEAYIPMAEKKLAQIGDEINEKWPGTITSIVHRIGPLQISDIAVLIAVSSPHRK
                     DAYRANEYAIERIKEIVPIWKKEIWEDGSKWQGHQKGNYEEAKREE"
     gene            20040..20273
                     /gene="moaD"
                     /locus_tag="O2701_001466"
     CDS             20040..20273
                     /gene="moaD"
                     /locus_tag="O2701_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HCZ4293365.1"
                     /translation="MKVLYFAEIKDILQKAQEDIVLEQALTVQQFEDLLFERYPQINN
                     KKFQVAVNEEFVQKSDFIQPNDTVALIPPVSGG"
     gene            20280..20879
                     /gene="mobA"
                     /locus_tag="O2701_001467"
     CDS             20280..20879
                     /gene="mobA"
                     /locus_tag="O2701_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCZ4293366.1"
                     /translation="MKAIILAGGHSVRFGKPKAFAEVNGETFYSRVIKTLESTNMFNE
                     IIISTNAQLATQFKYPNVVIDDENHNDKGPLAGIYTIMKQHPEEELFFVVSVDTPMIT
                     GKAVSTLYQFLVSHLIENHLDVAAFKEDGRFIPTIAFYSPNALGAITKALHSDNYSFK
                     NVYHELSTDYLDVRDVDAPSYWYKNINYQHDLDALIQKL"
     gene            20899..21921
                     /gene="moaA"
                     /locus_tag="O2701_001468"
     CDS             20899..21921
                     /gene="moaA"
                     /locus_tag="O2701_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCZ4293367.1"
                     /translation="MVEQIKDKLGRPIRDLRLSVTDRCNFRCDYCMPKEVFGDDFVFL
                     PKNELLTFDEMARIAKVYAELGVKKIRITGGEPLMRRDLDVLIAKLNQIDGIEDIGLT
                     TNGLLLKKHGQKLYDAGLRRINVSLDAIDDTLFQSINNRNIKATTILEQIDYATSIGL
                     NVKVNVVIQKGINDDQIIPMLEYFKDKHIEIRFIEFMDVGNDNGWDFSKVVTKDEMLT
                     MIEQHFEIDPVEPKYFGEVAKYYRHKDNGVQFGLITSVSQSFCSTCTRARLSSDGKFY
                     GCLFATVDGFNVKAFIRSGVTDEELKEQFKALWQIRDDRYSDERTAQTVANRQRKKIN
                     MNYIGG"
BASE COUNT         7574 a         3260 c         4097 g         7132 t
ORIGIN      
        1 ataaaaatat tgaattttta tacttatttg tttagaatga actttataac atagttggat
       61 agagtttcga tttaataaat tacatgtgaa ccttgctaca acaagatgtg catcagagga
      121 gtggtttaat aatgagtaaa attaatgaca ttaatgattt agtcaacgca acatttcaag
      181 ttaagaagtt tttcagagat acaaaaaaga agttcaattt gaactatgaa gaaatttata
      241 ttttaaatca tattttaaga agtgagtcta acgaaatctc atctaaagag attgctaagt
      301 gctcagagtt caaaccttac tatttaacta aagctttaca aaagctaaaa gatttaaaat
      361 tgttatcaaa gaaaagaagt ttacaagacg aaagaacagt tattgtttat gttacagata
      421 cacaaaaagc aaatattcaa aaactgattt cagaattaga agaatacatt aaaaattaaa
      481 tcaaggttaa ttgcgtttaa taacattgaa cgataacaat ttattaaaac gaagttattt
      541 attcagcatt gggacataaa attaacttaa aatttaaata ttgaagatgc tttaattaaa
      601 gttaaagact agccatacct tatttcagct tattaagctt gaaacaaggt acactagtct
      661 ttttatttta atattttctt agaaaatcaa gtttacgatc ataaatattt tctgcgatat
      721 agctttggat ggttccaagt attttctcta taatttgtgt gcgataagca aaaattctaa
      781 ctgcaaaacc atgtgtaggc aattgagaaa tagcaacacg acaatcgaat gtattgctat
      841 aagaactaat ggtttcataa actgaatcga ttagtttttg gttaacatct tcgtgtatga
      901 agtaagcaga gccataatga gagtaatgtt ccatataacc tatttcattg atcgattgtt
      961 tattaggatt caataataaa ttatcatatg tgactaactc atcttcaata taaatttcat
     1021 ttattaaatg catatattga tacttgaagg cttcgccagt ttttgaataa ccaggagtta
     1081 aaatatcagt ataaaataat gaactagaat tattgagatt gaacgtattg tgttgataaa
     1141 atttagcatt ttcatatgcg atgattggat cagcgacata ttctaaatat gcgttatctt
     1201 tcaaattaaa agtttgatac tgctcaacat gattggaagg tgtcttgtat atttttgttg
     1261 caccttgaga tgtcaatgtc actttagcgt tatcttcgac attcacattc atacggtaac
     1321 gatctccatc taaatagcca ccacctacat ttactatata aaatgtagga atggtagatt
     1381 gatttagata aactggacgt atcactttaa gtgctttttc aaagaaaata tctcttgata
     1441 ctgatcgatt gccatcgaaa aacactgtta aatcaagttg cccagtccat tgttgttcat
     1501 ccattatgat aatcctttga gtaaagtgtc gcgttcaatc caatcgataa cttcatccaa
     1561 accttcgtcg gtttttaagt tagtaaaagt aaatggacgt ttaccacgaa atactttagt
     1621 atcttcagcc atttgttcta atgatgcacc tacataggga gctaaatcag ttttgttaat
     1681 tacaaagaaa tctgacttaa tcataccttg accaccttta cgtggaatct tttcaccttg
     1741 agcaacatcg ataatatata ttgaaaagtc aacaagttct ggactaaatg ttgctgctaa
     1801 gttatcgcca ccagattcta tgaaaattag ttctatatcg tcatgacgtt ctaataattc
     1861 gtctattgct gcaaagttca tagatgcatc ttcacgaatc gcagtatgag gacatccacc
     1921 agtttcaaca ccaatgatac gactttcagg tagaactcct gaatttacta atatcttttc
     1981 gtcttctttt gtatatatat catttgtaat aacgccgata ctcatttctt ttgaaagacg
     2041 ttttacaact ttttcaatta attgtgtttt acctgcacct acaggaccac caataccaat
     2101 tttaatcgga tttgccacaa ttataacctc ctatgatatg aaaattctaa cattgacgtt
     2161 ctcatgcgcc atttgattta gttctaaacc aggcgctgtc atgccaaaat ctgcttcttt
     2221 taattcgaaa atctgctttc ttgttccttc tatataagga atcatgtgag taactatctt
     2281 ttgaccagca gtttgtccaa gtggaatagc acgaacagca ttttgagtta aacttgaaac
     2341 attttgatat aaatagtaat caataatcgt ttcaatatct acacctaaat gatggcctag
     2401 catagtaaaa caaatagctg gatttaactt tgctttctta tcttgcattt gttgatgata
     2461 ccaagcaatc catgggctat tataaagttc taaagccaat ttaaccatgc gagtccccat
     2521 ttgttttgca ccaacacgtg tttctttagg taagttttga acaaacatca gtttatctat
     2581 gtgtaatact ttttgtgtat catcattttc caatgcatca taaactaaac gcatggctaa
     2641 accatcagaa taggtaagtt gctcttgtaa aaacattttt aaccaagcaa taaaagtatg
     2701 atcgtcatga attatatttc gttgaatata tgtttcaaga ccaaatgaat gactgaaagc
     2761 acctgttgga aactgtgaat cacagaactg aaataatctt aagtgtgtat gatcaatcat
     2821 gagaatgccc tatatgtctg aaagccttat taactttacg gtcttctcga acatatggga
     2881 tgcctaaact ttttaataaa tcttcaacta aataatcata ttgtactagc atttcagtct
     2941 ctgtaaattg tgctggcaaa tgacgatttc ctaattgatg ggctatatct cccatttctt
     3001 gcaatgttct tggttgaatc actaaaagat cttctgaatt aacatccaca ataatcatat
     3061 tatggtcatc tgcgtataaa atatctccat attgtaagtc aataggttgt tttaaacgaa
     3121 tgcctatttc agtgccatgg tctgtaacga ctctttgaat acgtttaaca agatctgaat
     3181 tttcaaggta tactttttcg acgtgctttt gtttttctga atttgacaaa ttggcaatat
     3241 tgccttggat ttcttcaaca atcattctat gttcctccta gaataagaag tatctttgag
     3301 ttaatggtaa ctcagttgct gcattacttg taattttttc tccatctaca tatacttcat
     3361 atgtttgtgg atcaacgtct aatttaggtg ttgcactgtt atttttcata tctgctttag
     3421 ataattgtct aatattttta actggacgca ccatgcgttt taaatttaat gcacgattga
     3481 taccattttc ataagcagtt ttagacacga atgtcattga cgtacttgta aggtttccgc
     3541 cgtattgacc atacatttta cggtacttca tcggttcaga tgtaggtata gaaccatttg
     3601 catcgccatt tacggcagag ttaattaatc cgccctttac aactaattca ggtttaaccc
     3661 caaagaaaat tgggtcccat aagacaatgt cagctagttt gcccggctcg atagatccta
     3721 catattcaga aataccatgt gtaattgctg ggttaattgt atatttagcg atataacgtt
     3781 tgatgcgatt attatcatta tgttcaaaat caccatctaa aggaccacgt tgttctttca
     3841 tgcgatgtgc tacttgccat gttcgtgtaa ttacttcacc tacacggccc attgcttgtg
     3901 aatcggaact aatcatactg aatacaccca tatcttgcag aacatcttct gctgcaatcg
     3961 tttctttacg aatacgtgaa tctgcgaatg cgatatcttc aggaatagcc gcatttaaat
     4021 ggtgagtaat cattaccata tctaaatgtt catctacagt attatgtgta taaggcaaag
     4081 ttggatttgt agatgaaggt aaaatatttg aaaatgcagc ggatttaatt aaatcaggcg
     4141 catgaccgcc accagcacct tcagtatggt acatatgaag tacacggtct ttaacagcag
     4201 ccattgtgtc ttccataaat cctgcttcat ttaaagtatc tgcatgtaat gcaatttgaa
     4261 catcaaattc atcagcaaca tctaatgcat gactcaaagc agatggtgtt gcaccccagt
     4321 cttcatgtac ttttaatcca attgctccgg cattgatttg ttcaatgagt gcagttggat
     4381 ttgttgcttg tcctttacct gtaaaaccga cattaatcgg taaaccttcg gcagcttcta
     4441 acattctatg aatatgccat ggacctggag ttacagttgt tgctttagaa ccttctgaag
     4501 caccagtacc accaccaata tgagtcgtaa taccactttc taatgcgacc tctgcttgtt
     4561 caggattaat aaaatgaaca tgagtatcaa taccaccagc agtgacgatt ttaccttcag
     4621 cggcaatgat atctgttgtt gaacctataa taatgtcgac attatccatt atatctgggt
     4681 tgccggcatt acctatggcg aaaatataac catttttaat gcctatatca gctttaacca
     4741 ctttatcgta atcgataata acggcattag aaatgacaag gtctgcaacg ttcacgtcat
     4801 cacgtgttac acgaggattt tgcgccatac cgtctctaat agatttacca ccaccaaaag
     4861 tagcttcttc accataaacc gcatagtctt tttctatttg agcaaataga ttcgtatcac
     4921 ctaaacgaat ggaatctcca acagttggac cgtataagct cgtatattga ttttgcgtca
     4981 ttttaaagct catgatcttt ttcctccttt tttattcacg ttttcagcac cgttatctcc
     5041 gaatacacct gcatattcat cattttcatc agttgggcga tagacacgtg actcatcgat
     5101 aggaccattg accataccac gaaaaccaaa aattttacgt ttgccagcat attcaactaa
     5161 ttgaacttct tttttatccc caggttcaaa tcgaacagct gctccagctg gaatatctaa
     5221 atgttttcca tatgccattt cacgttcgaa atctaatgct gcatttgctt cataaaaatg
     5281 aaaatgtgag cccacttgaa ttggtcgatc tcctgtattt tcaacttcga taactgtttc
     5341 aggatgatgg ttattaattt caacctctgt actttttgta ataatttctc ctggtatcat
     5401 ttgactgcct cctttaaaca atagggtgat gtactgtgat taacttagta ccatcgggga
     5461 acgtagcctc gatttcgata tctgtaatca tgtgttcgac accatccatg acatcttctt
     5521 tgtttagaat ttgtctacca taactcatta actctgcaac ggtcttacca tcgcgtgcac
     5581 cttctaataa ttcatcgctg attaaagcta atgcctcagg atgatttagt ttcaaaccac
     5641 gtgctttacg acgacgtgca acttccgccg ccactacaat cattaatttg tcttgctctc
     5701 gttgtgtaaa atgcaaatta aaacccccaa tttcatatta gatacaattt acaaaattta
     5761 tattaatcct aattgttgtg ataaacaagt aatatacaaa gttcaatgtg taattagaaa
     5821 attatatttt tagcatatcc gatattgaag caaacaatct aatcgaaaac aaatagtgga
     5881 atatatttat gtaaaaacca aaatagtttt taatataact tttcatagaa tagtagtata
     5941 ttaatgagta atgattcaaa ggaaaggtga aagatttgaa gataatagat gtgcttttga
     6001 aaaatatatc tcaggttgtg ttaattagta ataaatggac aggattattt atcttaatag
     6061 gattatttgt agccgattgg acaattggat tagcggctat tgtaggtagc atcatcgcct
     6121 atacttttgc gcgttttata aattatagtg aggcagagat taatgatggg ttagctggat
     6181 ttaatccagt gctaactgcc attgcgttaa caatcttttt agataagtca ggattagata
     6241 ttgttataac aatgatagca actttattaa cgttaccagt tgctgctgca gtgagagaag
     6301 ttttaagacc atataaagtt ccgatgctga cgatgccttt tgtcattgtg acttggttta
     6361 caattttact ttcaggacag gttaaatttg tagatacatc gttaaagtta atgcctcaaa
     6421 acattgaaac ggttaatttt agcaacaatg atagaataca tttcattcag tcattatttg
     6481 aaggattcag tcaagtattt atcgaagcga gtgtaattgg tggcgtatgt attttaatcg
     6541 gcatattgat agcatcaaga aaagcaacac tcttagctgt tatagctagt ttgttaagct
     6601 ttatcattgt agctctatta ggtggtaatt atgatgatat taatcaggga ttattcggtt
     6661 ataactttgt attaatggca atcgcactag gatatacatt taaaacagcg attaaccctt
     6721 atatttcgac ttttttaggt gtgttattaa cagtagtggt gcaactaggt acaacaacat
     6781 tgcttgaacc gtttggctta cctgcattaa cattgccatt tattatcgtg acatggattt
     6841 tattatttgc tggtattaaa catgacaaag tagatgcttg atagttaaat caaacctaat
     6901 attgtttgaa tatcacctta aactatacag cgaattgtat agtttaaggt gtatttttat
     6961 ggataaaatt aagtgcatac ttaatcgaat tatcagtttt cttttaattg ttcacagcac
     7021 gttccttttt atttttaaag tcgagtgtta aactaggaat aaataattta tattgtgtgt
     7081 tgtgtggggt gactaatatg aatgatatgg ataattcctt tttaataaca acggaaattc
     7141 aaagaaaatg gattgaaaaa ttcaaagtaa ttagagatac atttaaggct aaagctgaat
     7201 ataatgatca acatagccaa tttccatata aaaatattga atggttaatt aaagaaggtt
     7261 atggaaaatt aacgttacca aaagcatatg gtggtgaagg tgcgaccata gaagacatgg
     7321 ttattttgca atcattttta ggcgaacttg atggtgccac agcattatct attggttggc
     7381 atgtgagtgt cgtaggacaa atttatgaac agaaattatg gtctcaagat atgttggagc
     7441 aatttgctgt tgaaattaat aatggtgcat tagttaatag agcagttagt gaagctgaaa
     7501 tgggtagtcc aacaagaggg ggaagaccaa gtacacatgc tgttaaagct gatgatgggt
     7561 atattttaaa tggtgtgaag acatatacat caatgagtaa agcactaaca catattattg
     7621 ttgctgctta tatagaagaa ttagaaagtg ttggtttttt cttagtagac aggaatttgc
     7681 ctggtgttga aattgccgat aattgggacg tattaggtat gcgagcgaca gaaagtcatg
     7741 atttaatatt aaatgatgta aaagtaccat taaaacattt agtggaaaca gagaaaagta
     7801 aagcgccaaa tggttggata ttgcatatac caagctgtta tttaggtata gcacaagctg
     7861 ctagaaatta tgctgtagac tttgcaatcc aacatagccc taatagtatt gaaggaacga
     7921 ttgcaacatt gccaactgtg caacaaaatt tagggaaaat ggaaacgcta ttgttatgtg
     7981 cgagacaatt tttatggagt acagcaaaag ggtatcaaca atataaagat gacagtcaga
     8041 taagaaatcc aacaagtgcg agtaaagtga tggtaatgaa tcaagggctt gaagtgattg
     8101 atttagcgat gagaatagtt ggtgctaaaa gcttagaaat gaatcgacca ttacaacgtt
     8161 actatcgtga catgcgtgca ggattacaca atccacctat ggaagatgca gcatatacaa
     8221 atattgctaa gagtattaca gatacatttt aaattttaaa aggtaaatcg atcgttgatt
     8281 ggcataaaga gatgaaattt gacgttcgga tttacccttt ttttgtgggg atttataggg
     8341 atgttaagat attttgttaa attttttgcg aaaataaaaa ataaataaca ctagctattg
     8401 taaatgtata tgcaatcttg tataattttt gaatgattga taatcatttt caataggagg
     8461 aaattatgaa aaaactatta ttaccattaa taattatgtt attagtgtta gctgcgtgtg
     8521 ggaaccaagg tgaaaaaaat aacaaagctg aaactaaatc ttataaaatg gacgatggca
     8581 aaacggtaga tattccgaaa gaccctaaac gcattgcagt agttgcgcca acatatgctg
     8641 gtggacttaa aaaattaggt gcaaacattg tagctgtaaa tcaacaagtc gatcaaagca
     8701 aagtattaaa agataaattt aaaggtgtta caaaaattgg tgatggcgat gtagaaaaag
     8761 ttgctaaaga aaagccagat ttaattattg tatactctac tgacaaagat attaaaaaat
     8821 atcaaaaagt agcaccaaca gtagttgttg actataataa gcataaatat ttagaacaac
     8881 aagaaatgtt agggaaaatt gttggtaaag aagataaagt aaaagcttgg aagaaagatt
     8941 gggaagaaac aactgctaaa gacggtaaag aaattaaaaa agcaattgga caagatgcaa
     9001 cagtgtcatt gtttgatgaa tttgataaaa aattatacac ttacggcgat aactggggtc
     9061 gtggtggaga agtattatat caagcatttg gtttgaaaat gcaaccagaa caacaaaagt
     9121 taactgcaaa agcaggttgg gctgaagtga aacaagaaga aattgaaaaa tatgctggtg
     9181 attacattgt gagtacaagt gaaggtaaac ctacaccagg atacgaatca acaaacatgt
     9241 ggaagaattt gaaagctact aaagaaggac atattgttaa agttgatgct ggtacatact
     9301 ggtacaacga tccttataca ttagatttca tgcgtaaaga tttaaaagaa aaattaatta
     9361 aagctgcaaa ataattcagc tatataagtt agtgaaatga gagtctgaaa catatcaatc
     9421 ttttgatatt gtattaggct cttattttta tagctagaaa gttagatatt tgtatttttt
     9481 taaataataa gtgccgttgt tatcgttcaa tttaattaat gatagattag tattattata
     9541 gctaaagtag tatacctgag aaaatagctc aatgtatctc tttattaata agttatatca
     9601 taattatttt agtgcatact ttatggaagg gatatcaggg aatggctttc aattaaagaa
     9661 gaggtttaaa aggattacaa cagaatgtta tgattttgta gaaagatata taacaacgtt
     9721 ttataaaaac ataatattgt taatggaaaa tgaaatgtaa gggggatttc gagtgactaa
     9781 gaaagtttat tttaaccacg atggtggtgt agatgattta gtatctctat ttttattatt
     9841 acaaatggaa aacgttcaat tgataggggt cagtacaatt ggtgctgatt gttatttaga
     9901 gccatctttg agcgcatcag taaaaattat taatcgtttt tcaaatgaag atattcaagt
     9961 tgcgccatca tatgaacgag gaaaaaatcc atttcctaaa gaatggcgta tgcatgcctt
    10021 ttttatggac gcattgccaa ttttaaatga gccagtcaaa catgttgctt caaatgtgag
    10081 cgacaaagaa gcctttgaag acattattca aactttaaag agacaatcag aaaaagtaac
    10141 attattattt acaggcccgc ttacagattt agcaaaagca ctacaaaaag attcatctat
    10201 cgttcagtat atagaaaaat tagtttggat gggtggcacc tttttaccaa aaggaaatgt
    10261 tgaagaacct gagcatgatg gttctgcaga atggaatgca tattgggatc cagaagcggt
    10321 taaaattgtt tttgatagcg atatagagat tgatatggtt gctttagaaa gtacgaatca
    10381 agtaccgcta acgttagatg ttagacaaag atgggcgaat gaacgccaat atacgggcat
    10441 tgatttttta ggagtaagtt atgcagcggt accaccatta acacacttta taacaaattc
    10501 tacttacttt ttatgggatg ttttaacgac tgcttatatt ggtaacaagg acttggttca
    10561 ttcaattgag aaaaaagtcg atgtaataag ttatggacca agtcaaggta agacatttga
    10621 gtgtaaagat gggcgcaaaa ttaatgtcat aaatcatgta gataacaacg cattttttga
    10681 ttatataact gcacttgcta aaaaagtaaa ttaacagctg tgtagaataa ttaaggtttt
    10741 aatttatata gaacaactta ttgtaaactt ttcatttctt aaagtttaca atggtgctat
    10801 aataatggtc atgaaatacg aaaggaagta aaaaatgaca acaaaacagt tagtatatac
    10861 agctttaatg acagcgatta tcgctatttt aggattggta ccggtaattc cactaccatt
    10921 ttcttcagta ccaattgtac ttcaaaacat tggtattttc ttagcaggtg tgattttagg
    10981 acgtaaatat ggcacattaa gtgttatcgt ctttttatta ttagtagttg ctggcttgcc
    11041 attgttatca ggtggtcgcg gtggcatcgg tgtattcgca ggtccttcag cagggttttt
    11101 actattatat ccagttgtag cattcatgat tggggcgatt cgagatagat tcatcaatga
    11161 aattaatttc tggattttat tcgttggtat tttagttttt ggtgttatag cattagatgt
    11221 tattggtaca ttgattatgg gcatgattat taacatacca tttacgaaag ctatttcaat
    11281 ttcattagct tatttgcctg gtgatatatt aaaagcaatt gtagcaagtt tgattggtac
    11341 agctttactt aatcactcgc agtttcgtca aattatggga ataaaataat catatttaag
    11401 atagtaaagt aattgaataa gttgctttga aatttataaa agtgaaagga gtaggtgtca
    11461 atggctagta taagtatgtc agatatatat tgtaacggca ctatatttga aaatgacgac
    11521 gagcagttga tttatttaac gccttctttt ccacaacgat acacaagtaa cacatggata
    11581 tataaaaaga cgcctaccca agagcgatgg ctgaaagact tagaacgtca acatcaatta
    11641 catacaaatc aaggttcaaa tcattatgcg tttagtttcc cggaaaatga acaacttgat
    11701 aatcattgga tggctatgtt taaagatatg aattttgaac taggtattat ggaattgtat
    11761 gccatagaaa gtgatgcgct tgccaatttg ccgcgtaact ctgacgttga aattgccatc
    11821 gttgacgagt cgcatataga tgcctattta aaagttgcat atcagtttag tttgccattt
    11881 ggaaaagact atgcagatgc acatgaagaa atggtaaggg aacattatca aaaagatgtg
    11941 attaaacgct tagtagctta tttaaataat gaacctattg gcgttgtaga tgtcattgaa
    12001 agtgaaaatt acattgaatt agatggattt ggtgtattag aacaatttcg gcaccaagga
    12061 attggatcta caattcaatc gttgataggt gaatacgcca tatcaaaaaa tcacaaacca
    12121 atcatattag ttgcagatgg tgaagataca gcaaaagata tgtatgcaaa gcaaggttat
    12181 gtctatcaat cgttttgtta tcaaatatta aaagaagata ttggaaatta acgattgatg
    12241 ataggtttgt attagaataa aaaagcagta agctggtttc gagtagaaat caacttactg
    12301 ctttttaaat tgttttgagc tacttatact tataaaaata gtgcgtttaa attgttgatt
    12361 catgtagaat atcgttcatt atgacacact ataatgaata tgttattgtt cagaatcaat
    12421 gatacgttct ggatgactgt atatattaaa gccaccattt cgaataaatc caactgccgt
    12481 aatatttagg tcattagcta aggttacagc aagcgttgtc ggagctgatt tagataaaat
    12541 gacgccaaca ccaatttttg cggctttaat taaaatttct gatgaaatac gtccactaaa
    12601 aattaatact ttatctcgga cagtaatatg tcgctgaata caaaatccat ataatttatc
    12661 tagagcgtta tgtctaccaa tgtcttgtcg atgtacaaaa aatgtcaaac catcgcttat
    12721 agcagcatta tgtaagccac ctgtttcttg gtaaatatga cttgcacttt gtaatcgagt
    12781 catcatgtta ataatttgca ttggagttaa agtgatttta gacatagatg ttttagcgat
    12841 agcagcatca ttttgaaaat aaaactcacg actctttccg caacaagatg caatcattcg
    12901 ttttgtggaa tattgaaagc gatcgcctaa atctttatta agttcaacat gggcaaaacc
    12961 tttactatca tcaatcagta cagattttaa ttcatctcgc tttaaaatgg caccttccga
    13021 agccagaaat ccaatgacta actcctcaag gtttgttgga ctgcatataa cagtcgcaaa
    13081 ttcttcacca ttcaccataa ttgtaagtgg aaattctgtc acatattgat ctgttgtatt
    13141 gaataatttt ccatcttcat atctaacaat tggttgacct aaagatacat ctttgttcat
    13201 tatctaaccc ctttaattag cttaaacttt attttaaagc aatttgctta aaattttaac
    13261 atatttgctt aagtttgaaa tttgattgat aaaaattaat agcgagcaat ctgtttgatt
    13321 taaattgaat tcgagaatat acatactagg gcatcaatta ataaatatca atcttatgca
    13381 aatttgacaa ttgtttgaat caatatataa acaggcaacg gttcttttca aatataatag
    13441 taagtgtata atgaaaatgt aaatattatt aaaaatgggg gttcactcaa tgaaattgaa
    13501 acgtttattt gctgttgtga ttgcaatgct tttagtatta gctggttgct ctaattctaa
    13561 cgataataat gaaagtaaaa aagatgacgc agacaatggt aagaaacaag agattcaagt
    13621 tgcagcggca gcaagtttaa cagatgtaac caagaaatta gcttcagaat ttaaaaaaga
    13681 gcataaaaat gctgatatta aatttaacta tggtggatca ggggcattaa gaaaacaaat
    13741 tgaatcaggc gcacctgttg acgtatttat gtctgcaaat actaaagatg tagatgcatt
    13801 aaaagacaag aataaagcgc atgatacata taaatatgcg aaaaatagtc tagtattaat
    13861 tggtgataaa gattcaaatt acacttcagt aaaagactta aaagacaatg ataaattagc
    13921 attaggtgaa gtgaaaactg taccagcagg aaaatatgcg aaacagtatt tagataacaa
    13981 taacttattt aaagaagtcg aaagtaaaat cgtttatgct aaagatgtaa aacaagtatt
    14041 aaattatgtt gaaaagggta atgcgaaaca aggttttgtg tataaaactg acttatataa
    14101 acaaaataaa aaaattgata ctgtaaaagt aattaaagaa gtagaactta agaagccaat
    14161 cacatacgaa gctggtgcta catcagatag taaattagca aaagagtgga tggaattctt
    14221 aaaatcagat aaagctaaag aaatactaaa agaataccac tttgcagcat aaggagttgt
    14281 aatccatgcc tgacttaaca cctttttgga tatcaatacg agttgctgta atcagtacga
    14341 ttattgtaac ggttttaggt atttttatat ctaaatggtt gtatcgtcgt aagggttcgt
    14401 gggttaaatt attggaaagt ttattgatat tacctattgt tttgccgcca acggtattag
    14461 gttttattct attaatcatc ttctcgccaa gaggaccaat cggtcaattc tttgcgaatg
    14521 tactacattt acctgtagtg ttcactttga caggtgctgt gatagcatct gtcattgtta
    14581 gttttccact aatgtatcaa catactgtgc aaggcttcag aggtatagac acgaaaatga
    14641 ttaatacagc tagaacgatg ggagcaagtg aaacgaaaat tttcctcaaa ttaattttac
    14701 cattagctaa acgctctatt ttagcaggta taatgatgag ttttgctcgt gcattaggtg
    14761 agtttggtgc tacattaatg gttgcaggat atattccaaa taaaacgaat acactacctt
    14821 tagaaatata cttcttagtg gaacaaggta gagaaaatga agcgtggtta tgggtattag
    14881 tgctagtcgc attctctatt gtggttatat ctacaattaa tttattgaat aaagataaat
    14941 ataaggaggt cgactagatg cttaaaatca atgtgaaata tcaattaaag aacactttaa
    15001 ttcgcatcaa tatagatgat actgaaccaa aaatttatgc agttcgtggt ccatctggca
    15061 ttggtaaaac tactgtttta aatatgattg ccggattacg taaagcagat gaagctatta
    15121 tcgaagtgaa tgggcaatta cttactgata cggcaaaaaa cgtgaatgtt aaaattcaac
    15181 aacgacgtat tggatatctg tttcaagact accaattgtt tcctaatatg acggtctata
    15241 aaaatattac ttttatggct gaaccatctg aacacatcga tcaattaatt caaactttaa
    15301 acattgatca tttgatgaaa caatatccta tgacattgtc aggtggagag gcacaacgtg
    15361 tagcacttgc acgtgcactt agcacgaaac cagatttaat tttattagat gaaccttttt
    15421 ctagtttgga tgatactaca aaagatgaga gtattacatt agttaaacgt attttcaacg
    15481 aatggcaaat accaatcata tttgtgacac attcaaacta tgaagcagaa caaatggctc
    15541 atgaaattat tacaattggg taatcattta tttgccatta aatagtttag aacgtattta
    15601 aaattgtaga agtgaatgct tctatcagca ttttaatgat gttttaaact cttttttagg
    15661 ggcagttttt ttgagagaca ttgacgcgcg tcatataatg aaagtaatga taaaaagaaa
    15721 ggataactta atgtgagtca agaacgttat tcaaggcaaa ttttatttaa acaaataggt
    15781 gaaataggtc aaagcaaaat aaatcaaaaa tgtgcgttga ttattggtat gggcgcatta
    15841 ggtacacatg tggccgaagg acttgttaga gcaggcattg ccaaactaat cattgttgat
    15901 agagattata ttgaatttag taatttacaa agacaaacat tgtttactga agaagatgct
    15961 ttgaaaatga tgcctaaggt ggttgcagct aaaaagcatt tgctagcgtt acgtagtgat
    16021 gttgatattg atgattatat tgcccatgtg gattattatt ttttggaaac acatggacag
    16081 gacgttgacg ttattattga tgcaaccgat aactttgaaa cacgacaact gattaatgat
    16141 tttgcatata aatatcgtat accttggatt tatggtggtg ttgtacagag tacatataca
    16201 gaagctgcat ttatacctgg taaaacacct tgctttaact gtttggtacc acaattgcca
    16261 gcattaaatt taacatgtga tacagtaggg gtcattcaac ctgccgtgac gatggcaaca
    16321 agtttacaat taagagatgc gatgaaagta ttaacggaac aaccaattga cacaaaaata
    16381 acttatggcg atatttggga aggtagtcat tattcatttg gtttcagtaa aatgcaacgt
    16441 tcagactgta caacttgtgg agatgtacca agttatccgt atttaaacaa gaatgaacaa
    16501 cgttatgcaa cattgtgtgg tagagacact gtacagtatg aaaatgcatc aattacacac
    16561 gacattcttg ttcaattttt aaaacaacat cagttaaatt atcgcagtaa ttcgtatatg
    16621 gttatgtttg aatttaaagg acaccgcatt gttgctttta aaggtggaag gtttttaata
    16681 catggcatga cacgcacatc agatgccaca catctaatga atttattgtt tggataaaaa
    16741 aagataagac aaaaggagtg taatattatg ggcgaacatc aaaacgttaa attgaatcgt
    16801 acagttaaag cagccgtact aacggtatca gatactagag actttgatac agataaaggt
    16861 ggtcaatgcg tgcgccaact attacaagca gatgacgttg aagtgagtga cgcacattat
    16921 acaattgtga aagatgaaaa agtagccatc acgacgcagg tgaagaagtg gttagaagaa
    16981 gatattgatg tcatcattac gactggtgga acaggtattg cacaacgtga tgtgacgatt
    17041 gaagcagtaa aaccactttt aactaaagag atagatggct ttggggaatt gtttagatat
    17101 ttgagttatg ttgaagatgt tggcacgcgt gcattattgt ctcgtgctgt agcaggtaca
    17161 gttaataata aattgatatt ttcgattcca ggatcaacag gcgcagttaa attagcatta
    17221 gaaaagctca ttaaaccaga attaaatcat ctgattcatg agcttacaaa ataatttatt
    17281 gatttgattg gcgttgaaaa tctccagatt taccgccaga cttgctttca aggtaggttt
    17341 cgccaataat cataccttta tcaactgctt tcgtcatgtc gtaaatggtt aaagccgttg
    17401 ctgatgcagc ggttaaagct tccatttcaa caccggtttt gccagttgta gagacagttg
    17461 tttgaatgtt taaagtataa aggggtgcat ttgtttcatc ccagctgaag tgaacatcta
    17521 tgccagtcaa tggtaatgga tggcacatcg gaataagtgt tgatgtattt ttggcagcca
    17581 taataccagc gatttgagca gtgttcaata cattgccttt ggtattggtg ttattagaaa
    17641 tctgcttaaa aatagtttca tttacagtaa tgcttgaatg agcgacagca gttctttttg
    17701 taatttgttt gtctgataca tcgaccattt tggcgtggcc ttgttgatta atatgagtaa
    17761 actcagtcat tttacccctc ctagtgcatc tagtatatca tgaaaaaata aaagttttgg
    17821 agatgatttt taatggtagt agaaaaaaga aacccaatcc cagttaaaga agcaattcaa
    17881 cgtatcgtta atcagcagag ttcaatgccg gcaattacgg tagcacttga aaaaagtcta
    17941 aatcatatct tagcagaaga tattgtagct acttatgata taccaaggtt tgataaatca
    18001 ccttatgatg gttttgcaat tcgcagtgtt gattcacaag gggcaagtgg tcagaatcgc
    18061 attgagttta aagtgattga tcatattggt gcaggttcag tttctgataa attagttggg
    18121 gatcacgaag cggtgcgtat tatgactgga gcacaaatac ctaatggcgc agatgctgtt
    18181 gttatgtttg aacaaacgat tgaactagaa gatacattta caattcgtaa accattttca
    18241 aaaaatgaaa atatatcttt aaaaggtgaa gaaacaaaga caggcgatgt tgttctaaaa
    18301 aaaggacaag taattaatcc aggggctatc gcggtccttg caacatatgg ctatgcagag
    18361 gttaaagtta ttaagcaacc gagtgtcgct gttattgcaa caggaagcga attattagat
    18421 gttaatgatg tattagaaga tggaaaaatt cgtaactcta atggcccaat gattcgtgcc
    18481 ttagcagaaa aattaggtct tgaagttggt atttacaaaa cacaaaaaga tgatttagat
    18541 agtggcatcc aagtcgttaa agaagctatg gaaaaacatg atatcgttat tacaacgggc
    18601 ggagtttctg ttggagattt tgactattta cctgagattt ataaggctgt aaaggcggaa
    18661 gtgttattta ataaagtagc aatgcgtcct ggtagcgtaa caacggttgc atttgtagat
    18721 ggaaagtatt tgtttggatt atctggaaat ccatcagctt gttttacagg atttgaacta
    18781 tttgtgaaac cagctgttaa acatatgtgt ggcgcactag aagtcttccc gcaaataatt
    18841 aaagcaacat taatggaaga ttttaccaag gcaaacccat tcacacgatt tatacgtgct
    18901 aaagcaacgt taacaagtgc tggagctact gtagtacctt caggattcaa taaatcaggt
    18961 gcggttgtag cgattgcaca tgctaactgt atggtcatgt taccaggagg gtcacgtggt
    19021 tttaaagcgg ggcatacagt agatattata ttgactgaat ctgacgctgc tgaagaggaa
    19081 cttcttttat gattttacaa attgtaggtt acaaaaagtc tggtaagaca acattgatga
    19141 ggcatattgt ctctttctta aagtcacatg gttatacagt tgctactatt aaacatcatg
    19201 ggcatggtaa ggaagatatt caattacagg attcagacgt cgatcacatg aagcattttg
    19261 aagcgggggc agatcaaagt attgtacaag gttttcaata tcagcaaact gtaacacgtg
    19321 tagataatca aaatcttact caaattattg aaaaatctgt tacaattgac accaatatcg
    19381 tattagttga aggctttaaa aatgctgatt ttgaaaaagt cgtagtctat cgaaatgaag
    19441 aagagttgca agtattacaa caattgtcga atgtttgtta tagcattaat gtaagggagc
    19501 atgaagattt tacagcattt gagcaatggt tattaaataa aattaaaaat gattgtgata
    19561 cacaattaac atagaggatt gaaatgaatg aaacaatttg aaatcgtgac agaaccgata
    19621 caaacagaac aatatcgtga attcactata aatgaatatc aaggtgcagt agttgttttt
    19681 accggtcatg ttcgcgaatg gactaaaggc gtcaaaacgg aatatttaga atatgaagcg
    19741 tatattccaa tggctgaaaa gaaattggca caaattggag atgaaataaa tgaaaaatgg
    19801 cctggaacga taacgagtat tgttcataga atagggccat tacaaatttc agatatcgct
    19861 gtattaattg cggtttcttc accgcatcgt aaagatgcct atcgagcaaa tgaatatgca
    19921 attgagcgta taaaagaaat tgttccgatt tggaaaaaag aaatttggga agatggttca
    19981 aaatggcaag ggcatcaaaa agggaattat gaagaagcga agagggagga ataagagaga
    20041 tgaaggtact ttacttcgca gaaattaaag atatattaca aaaagcacag gaagatattg
    20101 tgcttgaaca agcattgact gtacaacaat ttgaagattt attgtttgaa cgttatccgc
    20161 aaatcaataa taaaaagttt caagttgctg taaatgagga atttgtacaa aaatcggatt
    20221 tcattcaacc taatgatact gttgcattaa ttccaccggt tagtggaggt taagggagca
    20281 tgaaagcaat aattcttgca ggtggtcatt cagtgcgatt tggtaagccc aaagcttttg
    20341 cggaagtgaa cggtgagacc ttttatagta gagtaattaa gacattagaa tcaacaaata
    20401 tgttcaatga aattattatt agtacaaatg cgcaattggc aacgcaattt aaatatccaa
    20461 atgttgttat agatgatgag aatcataatg ataaaggtcc attagcagga atttatacaa
    20521 tcatgaagca acatcctgaa gaagaattgt tttttgtcgt ttctgttgat acaccaatga
    20581 ttactggtaa agctgtaagc acgttgtatc agtttttagt ttctcatctt attgaaaatc
    20641 atttagatgt cgcagctttt aaagaagatg gacgttttat tccaacaatt gcattttata
    20701 gtccgaatgc attaggcgct ataactaaag cactacattc tgataattac agttttaaaa
    20761 atgtatatca tgaattatca acggattatt tggatgtaag ggatgtagat gcgccctcat
    20821 attggtacaa aaatataaat tatcagcatg atttggacgc tttaattcaa aaattgtaag
    20881 ctgttaggag gtccacaaat ggtagaacaa ataaaagata aactaggacg tcccatccgt
    20941 gacttacggt tatctgtgac agatcggtgt aactttaggt gtgattattg catgcctaaa
    21001 gaggtatttg gagatgattt cgtattttta cctaaaaatg aacttttaac gtttgatgaa
    21061 atggctagaa tcgctaaggt atatgcagaa ttaggtgtaa aaaaaatacg cattacaggt
    21121 ggagaaccat tgatgcgacg ggatttagat gtacttatag ctaaattaaa tcaaatcgat
    21181 ggtattgaag atattggttt gactacaaat ggtttgttat taaaaaagca tggacaaaag
    21241 ttatatgatg ctgggctgcg cagaattaat gtcagtttgg atgctattga tgatacgcta
    21301 tttcaatcaa tcaataatcg taatattaaa gcgactacga ttttagaaca aattgattac
    21361 gcgacgtcta ttggtttgaa tgtaaaagta aatgttgtta tacaaaaagg tattaacgat
    21421 gatcaaatca taccaatgct tgaatatttt aaagataaac atatagagat tcgatttata
    21481 gaatttatgg atgttggtaa tgataatgga tgggatttca gtaaagttgt aactaaagat
    21541 gaaatgctta caatgataga gcagcacttt gaaatcgatc ctgtagaacc aaaatatttt
    21601 ggggaagtag caaaatatta tcgccataag gataatggtg ttcaatttgg tttgattaca
    21661 agtgtttcac aatcattttg ttctacatgt acacgcgcaa ggctgtcatc agatgggaag
    21721 ttttacggat gtttatttgc aactgtcgat ggatttaacg ttaaagcgtt tattcgttct
    21781 ggcgtgaccg acgaagaatt aaaagaacaa tttaaagctt tatggcaaat aagagatgat
    21841 cgatattcag atgagagaac tgctcaaaca gttgccaatc gtcaacgtaa aaagataaac
    21901 atgaattata ttggtggtta atgtgtaggg accactacat attaaatcat tagagatgtt
    21961 ttaatatttc tgtcttactc cctaaaatac aatattattt attaaagtaa aaacggtcat
    22021 atctatgcca gatttaatag aaatgatcgt ttttaaagtt ttt
//
LOCUS       DALAGD010000049        21751 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.049, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000049 DALAGD010000000
VERSION     DALAGD010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 21751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21751
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.049"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            64..573
                     /locus_tag="O2701_001469"
     CDS             64..573
                     /locus_tag="O2701_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcG family protein"
                     /protein_id="HCZ4293368.1"
                     /translation="MILGLALIPSKSFQEAVDSYRKRYDKQYSRIKPHVTIKAPFEIK
                     DGDLDSVIEQVRARINGIPAVEVHATKASSFKPTNNVIYFKVAKTDDLEELFNRFNGE
                     DFYGEAEHVFVPHFTIAQGLSSQEFEDIFGQVALAGVDHKEIIDELTLLRFDDDEDKW
                     KVIETFKLA"
     gene            complement(686..1876)
                     /locus_tag="O2701_001470"
     CDS             complement(686..1876)
                     /locus_tag="O2701_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ4293369.1"
                     /translation="MQDSSLNNYANHKNFILMLIILFLMEFARGMYILSYINFLPTVT
                     SIAVAITSLAFSIHFIADASTNFVIGFLLKKFGTKIVLTTGFILAFTSLFLVIWFPAS
                     PFVIIFSAMMLGIAVSPIWVIMLSSVEEDKRGKQMGYVYFSWLLGLLVGMVFMNLLIK
                     VHPTRFAFMMSLVVLIAWILYYFVDVKLTNYNTRPVKAQLRQIVDVTKRHLLLFPGIL
                     LQGAAIAALVPILPTYATKVINVSTIEYTVAIIIGGIGCAVSMLFLSKLIDNRSRNFM
                     YGVILSGFILYMILIFTLSMIVNIHILWIIALAIGLMYGILLPAWNTFMARFIKSDEQ
                     EETWGVFNSIQGFGSMIGPLFGGLITQFTNNLNNTFYFSALIFLVLAVFYGSYFIVNR
                     EKAK"
     gene            complement(1854..3029)
                     /locus_tag="O2701_001471"
     CDS             complement(1854..3029)
                     /locus_tag="O2701_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diglucosyl diacylglycerol synthase"
                     /protein_id="HCZ4293370.1"
                     /translation="MVTQNKKILIITGSFGNGHMQVTQSIVNQLNDMNLDHLSVIEHD
                     LFMEAHPILTSICKKWYINSFKYFRNMYKGFYYSRPDKLDKCFYKYYGLNKLINLLIK
                     EKPDLILLTFPTPVMSVLTEQFNINIPVATVMTDYRLHKNWITPYSTRYYVATKETKQ
                     DFIDVGIDPSTVKVTGIPIDNKFETPINQKQWLIDNNLDPDKQTILMSAGAFGVSKGF
                     DTMITDILAKSANAQVVMICGKSKELKRSLTAKFKSNENVLILGYTKHMNEWMASSQL
                     MITKPGGITITEGFARCIPMIFLNPAPGQELENALYFEEKGFGKIADTPEEAIKIVAS
                     LTNGNEQLTNMISTMEQDKIKYATQTICRDLLDLIGHSSQPQEIYGKVPLYARFFVK"
     gene            3462..4943
                     /locus_tag="O2701_001472"
     CDS             3462..4943
                     /locus_tag="O2701_001472"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HCZ4293371.1"
                     /translation="MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVG
                     YTVDSHKFCQNVADQGCKLVVVNKEQSLPANVTQVVVPDTLRVASILAHTLYDYPSHQ
                     LVTFGVTGTNGKTSIATMIHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLT
                     KKIKEAVDAGAESMTLEVSSHGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHA
                     KSLLFSQLGEDLSKEKYVVLNNDDSFSEYLRTVTPYEVFSYGIDEEAQFMAKNIQESL
                     QGVSFDFVTPFGTYPVKSPYVGKFNISNIMAAMIAVWSKGTSLETIIKAVENLEPVEG
                     RLEVLDPSLPIDLIIDYAHTADGMNKLIDAVQPFVKQKLIFLVGMAGERDLTKTPEMG
                     RVACRADYVIFTPDNPANDDPKMLTAELAKGATHQNYIEFDDRAEGIKHAIDIAEPGD
                     TVVLASKGREPYQIMPGHIKVPHRDDLIGLEAAYKKFGGGPVD"
     gene            4927..5187
                     /locus_tag="O2701_001473"
     CDS             4927..5187
                     /locus_tag="O2701_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueH family protein"
                     /protein_id="HCZ4293372.1"
                     /translation="MALLINKRFIDEGKTIDVYLFEALNNQIIIAIPDWFWSYQMAMT
                     LDEETCFEAILMQLFVFKEEEEAESIASQLTDWIETYKKEKD"
     gene            5187..6749
                     /locus_tag="O2701_001474"
     CDS             5187..6749
                     /locus_tag="O2701_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCZ4293373.1"
                     /translation="MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVK
                     GKKTGKFATSDWMKVEQERGISVTSSVMQFDYDDYKINILDTPGHEDFSEDTYRTLMA
                     VDSAVMVIDCAKGIEPQTLKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLN
                     IETYPMNWPIGMGQSFFGIIDRKSKTIEPFRDEENILHLNDDFELEEDHAITNDSDFE
                     QAIEELMLVEEAGEAFDNDALLSGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQ
                     TKEDVEVSPFDDSFSGFIFKIQANMDPKHRDRIAFMRVVSGAFERGMDVTLQRTNKKQ
                     KITRSTSFMADDKETVNHAVAGDIIGLYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEI
                     FMKVSAKNVMKQKHFHKGIEQLVQEGAIQYYKTLHTNQIILGAVGQLQFEVFEHRMKN
                     EYNVDVVMEPVGRKIARWIENEDQITDKMNTSRSILVKDRYDDLVFLFENEFATRWFE
                     EKFPEIKLYSLL"
     gene            7051..7854
                     /locus_tag="O2701_001475"
     CDS             7051..7854
                     /locus_tag="O2701_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCZ4293374.1"
                     /translation="MLMDPSLILPYLWVLVVLVFLEGLLAADNAIVMAVMVKHLPPEQ
                     RKKALFYGLLGAFVFRFLALFLISIIANFWFIQAAGAVYLIYMSIKNLWQFFKHPEIE
                     SPEAGDDHHYDESGEEIKASNKSFWGTVLKIEFADIAFAIDSMLAALAIAVTLPKVGI
                     HFGGMDLGQFVVMFLGGMIGVILMRYAATWFVELLNKYPGLEGAAFAIVGWVGVKLVV
                     MVLAHPDIAVLPEHFPHGVLWQSIFWTVLIGLVIIGWLGSVVKNKKSHK"
     gene            8073..10397
                     /locus_tag="O2701_001476"
     CDS             8073..10397
                     /locus_tag="O2701_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCZ4293375.1"
                     /translation="MEWTLVDIGKKHVIPKSQYRRKRREFFHNEDREENLNQHQDKQN
                     IDNTTSKKADKQIHKDSIDKHERFKNSLSSHLEQRNRDVNENKAEESKSNQDSKSAYN
                     RDHYLTDDVSKKQNSLDSVDQDTVKSKYYEQNSEATLSTKSTDKVESTEMRKLSSDKN
                     KVGHEEQHVLSKPSEHDKETRIDSESSRTDSDSSMQTEKIKKDSSDGNKSSNLKSEVI
                     SDKSNTVPKLSESDDEVNNQKPLTLPEEQKLKRQQSQNEQTKTYTYGDSEQNDKSNHE
                     NDLSHHIPSISDDKDNVMRENHIVDDNPDNDINTPSLSKTDDDRKLDEKIHVEDKHKQ
                     NADSSETVGYQSQSTASHRSTEKRNISINDHDKLNGQKTNTKTSANNNQKKATSKLNK
                     GRATNNNYSDILKKFWMMYWPKLVILMGIIILIVILNAIFNNVNKNDRMNDNNDADAQ
                     KYTTTMKNANNTVKSVVTVENETSKDSSLPKDKASQDEVGSGVVYKKSGDTLYIVTNA
                     HVVGDKENQKITFSNNKSVVGKVLGKDKWSDLAVVKATSSDSSVKEIAIGDSNNLVLG
                     EPILVVGNPLGVDFKGTVTEGIISGLNRNVPIDFDKDNKYDMLMKAFQIDASVNPGNS
                     GGAVVNREGKLIGVVAAKISMPNVENMSFAIPVNEVQKIVKDLETKGKIDYPDVGVKM
                     KNIASLNSFERQAVKLPGKVKNGVVVDQVDNNGLADQSGLKKGDVITELDGKLLEDDL
                     RFRQIIFSHKDDLKSITAKIYRDGKEKEINIKLK"
     gene            10414..11772
                     /locus_tag="O2701_001477"
     CDS             10414..11772
                     /locus_tag="O2701_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCZ4293376.1"
                     /translation="MSIFSQFLKRSSPQQGIVLYYIVAIVIAFLLLNLPYVHKPGVEV
                     NPIDTLFVAVSGISVTGLSPISIVDTYSTFGQLIILVILNIGGIGVMAIGTMLWVVLG
                     KHIGIRERQLIMLDNNKNTMSGTVKLIIDIVKSIFVIELVGAMLLAFYFYRDNPDLKY
                     AIMQGVFVSISATTNGGLDITGKSLIPYAHDYFVQAIVIFLIILGSIGFPVLLEVKAY
                     IQNRVTNFRFSLFTKITTSTYLFLFIVGVLAILLFEHNHAFKGLSWHQSLFYSLFQSA
                     TTRSAGLQTIDVTTLSDPTNIIMGILMFIGSSPSSVGGGIRTTTFAILILFLINFSNN
                     ADKTSIKVYNREVHIMDIQRSFAVFTMATILTFLGMLIISATENGKLTFLQVFFEVMS
                     AFGTCGLSLGVTSDISDISKVVLMILMFIGRVGLISFIIMIAGRREPDKFHYPKERIQ
                     IG"
     gene            11911..13422
                     /locus_tag="O2701_001478"
     CDS             11911..13422
                     /locus_tag="O2701_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCZ4293377.1"
                     /translation="MEKNENINVEILTTSDMHSHFLNGDYGSNIYRAGTYVNQVRAQN
                     HRVILLDSGGSLAGSLAAYYYAIVAPYKRHPMIKLMNRMHYDASGVSPSEFKFGLSFL
                     TRSIALARFPWLSANIEYNVTKEPYFSTPYCIKHFGDLKIAIVGVTADGLMENEYSEM
                     EQDVSIEKTLVASKRWIRYIHEVEEPDFLIVIYHGGLNKISNSTKNKKASSNEAEKLM
                     EELGVIDLMITAHQHQTIVGQDHETYYVQAGQDAKELVHLSINFKKRTTTYDVESIDS
                     KVIDLNEYEEDQELLDLTFYDRKAVAYWSQEIISDKGLMLSVNGLQDLVCQTHPFSQL
                     LHDAIHLAFDNDITCVHVPMNGEKGLSGQIRNEDLYHAYPYPDKPMDMTISGQNIKDI
                     LEYSYSHLDFVNEQLSLTIIDETLCTMWQGFNYEIDMNQEPGQRVMLDQIDLTKSYRV
                     TMTDYCYRNYKNYLKNAIIHESYDETMSTLIAEKLRDPNYRISCSDNFIVKNR"
     gene            complement(13602..13690)
                     /locus_tag="O2701_001479"
     tRNA            complement(13602..13690)
                     /locus_tag="O2701_001479"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13653..13655),aa:Ser,seq:gct)
     gene            complement(13693..13767)
                     /locus_tag="O2701_001480"
     tRNA            complement(13693..13767)
                     /locus_tag="O2701_001480"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13733..13735),aa:Asn,seq:gtt)
     gene            complement(13909..14478)
                     /locus_tag="O2701_001481"
     CDS             complement(13909..14478)
                     /locus_tag="O2701_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HCZ4293378.1"
                     /translation="MYSQNIYVIRKGDMVIRPAFDDDDQRNGSEIIRFDKTRIQNPFK
                     VQKIIERSCKFYGNTYLGKKAETNRITGISSKPPILLTPLFPTYFFPTHSDRQNENIW
                     LNMHYIESIKELKNRKCKVTFINNESIILHVSYHSLWHQYNNSIFYYYMVDKQSRMIS
                     KNPDQPIDYNKATLNVFEALTRYSLFEDK"
     gene            14688..14906
                     /locus_tag="O2701_001482"
     CDS             14688..14906
                     /locus_tag="O2701_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HCZ4293379.1"
                     /translation="MKYNTNVKHTTLEAFVTTVNDLGIELIINEALREVRKRQLIELI
                     DDALVNKDEAAFNQYTAEYKNLEAFLGE"
     gene            complement(14987..15973)
                     /locus_tag="O2701_001483"
     CDS             complement(14987..15973)
                     /locus_tag="O2701_001483"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCZ4293380.1"
                     /translation="MKFISNNNITDPTLNLAMEEYVLKNLPAEESYFLFYINRPSIIV
                     GKNQNTIEEVNQTYIDAHNIDVVRRISGGGAVYHDTGNLNFSFITDDDGNSFHNFQKF
                     TEPIVQALQSLGVNAELTGRNDIQVGQAKISGNAMVKVKNRMFSHGTLMLNSDLDEVQ
                     NALKVNPAKIKSKGIKSVRKRVANIQEFLNDPLEIEEFKKIILKTIFGETEVEEYKLT
                     DEDWENIEKLSNDKYRTWEWNYGRNPKYNFEREEKFEKGFVQIKFDVKRGKIEHAKIF
                     GDFFGVGDVTDLENALVGCLHDFEHIEEALSEYDLYHYFGDIDRHELIRLMS"
     gene            16172..16348
                     /locus_tag="O2701_001484"
     CDS             16172..16348
                     /locus_tag="O2701_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkvS family protein"
                     /protein_id="HCZ4293381.1"
                     /translation="MTVAEVGNIVEFMDGLRGRVEKINDNSVIVDLTIMENFNDLDLP
                     EKTVINHKRYKIVE"
     gene            16363..16965
                     /locus_tag="O2701_001485"
     CDS             16363..16965
                     /locus_tag="O2701_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCZ4293382.1"
                     /translation="MNKISKALTWFIISFIIFHLILFIMWGEHQEYWYLYTGIMLIAG
                     ISYVFYQRDIESKRLLTSIGVGIITAIILIMLQLLFSLITSNLSYSSLIKELARTGVN
                     WKWQMLVTLLFVIPCHELYMRTVLQKELTHFSIPKWVAILITAICSSSLFIYLDNWWI
                     VTFIFVAQVILSLSYEYTRRIATTSVAQIVAIILLLIFNA"
     gene            17198..17343
                     /locus_tag="O2701_001486"
                     /pseudo
     CDS             17198..17343
                     /locus_tag="O2701_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500662.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
     gene            17882..17983
                     /locus_tag="O2701_001487"
     CDS             17882..17983
                     /locus_tag="O2701_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293383.1"
                     /translation="MKFAANKLTLTIFRKFCINNEKEIWIKYEEVFL"
     gene            17993..18265
                     /locus_tag="O2701_001488"
     CDS             17993..18265
                     /locus_tag="O2701_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HCZ4293384.1"
                     /translation="MFGTILYLTLALGLSTAAYASTEYAEGGTWSHGVGSKYVWSYYY
                     HGHKGHGATAIGKYRSFSGYTRAGVKAKASATKHNWWVNRAYYNIY"
     gene            18309..20273
                     /locus_tag="O2701_001489"
     CDS             18309..20273
                     /locus_tag="O2701_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HCZ4293385.1"
                     /translation="MKWFKLILDVTIFILIAILLFVYTYKENEEILPDTKYPIAVTDW
                     NKKYSKNEIYKRIDQFAKNENVAIYKSTSNYTNKNVDKDIYVFNKSKAATITPFNAKY
                     NIHYLSDDEVLKKDIKGSYFVKDKNFDVSKFINFLKEYGVTAESYKIDHMMIAVGVIK
                     QMNIVVPLSSLLIVYFIYYIFEKNINFKAYAIKYLNGFTLRKIIFENFSKKCTYWVTL
                     IITQILLTTSVLWILNYTGNLDLFILRIVLLSCLFILTISVINLWTFLMLLNLNIANM
                     IKGKQHFKTIRFINTVCKSILLVLIANVMIENTSVIKDLNKIKETEKYWNVLDDYYTI
                     EFAPYHETKQSLIDNMVRSEQLVKASEAENNAILFKPKGDSVDNDNFSPDEGNVILVN
                     NQFWSIYYKQFQPDIPIKNQKNNVEVIIPQKFHAMRNEINQAYHSWFEFVQNKNNKEN
                     KLSIQFINKNDCRIFSFDARDSRHLSFIEAPIIVNVQASDLSNDFYYAMISQGGYLFK
                     NYDALVKNIEKYHLDGEISGITNYKDSVMEMYHENNLKLTVLNFSQIIIAIILIIIIL
                     FDVKYYFEQHRKLLVIKKLYGYSTLRANYQYLLINNIVVIFIGILTNVILHSHYIMML
                     FATILVVQILLQICSLYYHGRRFNEVIKEF"
     gene            20276..20596
                     /locus_tag="O2701_001490"
     CDS             20276..20596
                     /locus_tag="O2701_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCZ4293386.1"
                     /translation="MKFIIAILLGLILSITIAFTIIHHPILDRFNPFLKTEYSYAKVP
                     KGTQQYVNITAYSERGEKLDYKLTFNGFSPSRTYVEIKHKGQYVISITYVEKEDTPKE
                     VRQE"
     gene            20593..21234
                     /locus_tag="O2701_001491"
     CDS             20593..21234
                     /locus_tag="O2701_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4293387.1"
                     /translation="MIELKDLTIQKGNTHILKKLNLKFQCGKSYALIGKSGCGKSTLL
                     NTIAGLEKTGKQYVYFNGQLEQFKSNFYRDKLGYLFQNYGLIDNLTVNENLDIGLAYK
                     KISKKEKEQIKIRYIEQFGLSNSLKRKVHTLSGGEQQRVALIRMMLKDPIVMLADEPT
                     GALDPKTGQMIIQSLFGLVDENKVLILATHDMAIANQCDEIIDLEQYRKVASM"
     gene            complement(21322..21612)
                     /locus_tag="O2701_001492"
     CDS             complement(21322..21612)
                     /locus_tag="O2701_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293388.1"
                     /translation="MFYACHFKVKIGGVILTRTYNIIGILSCLISFIIMALPMIWYTA
                     SALWFFPGAIMILLLSLVIVFCYIKTKNQLHLLLIVLNIIILLFFSLPLLLS"
BASE COUNT         7833 a         2981 c         3762 g         7175 t
ORIGIN      
        1 aatgtaacgg ttttcaaaga aatttgatat aatagcaata ggttaaacaa aggaggaatt
       61 cagatgattt taggattagc attaattcca tcaaagtcat ttcaagaagc ggtggattct
      121 taccgtaaaa gatatgataa acagtattca cgaattaaac cacatgtgac aattaaagcg
      181 ccatttgaaa ttaaagatgg tgatttagat tctgtcattg aacaggttag agctcgtatt
      241 aatggtatac cagcagtaga agttcatgct acaaaagctt ctagcttcaa accaacgaac
      301 aatgtgattt actttaaagt tgcgaagacg gacgacttag aagaattgtt taatcgcttt
      361 aatggagaag atttctatgg agaagctgaa catgtttttg tgccacactt tacaatagca
      421 caaggactat ctagccaaga attcgaagat atttttggtc aagtagcatt agctggggta
      481 gaccataaag aaattatcga tgaattaact ttgttacgtt ttgacgatga cgaagataaa
      541 tggaaagtta ttgaaacgtt taaattagct taagtaacat aatagtattg ttaatcgtag
      601 tatgtttgaa ttaataagaa aatggtcatt tttattgaat gtaataaaaa tgaccatttt
      661 ctttatttta aaatacgttt taaccttact tagctttttc tctatttact ataaagtagc
      721 ttccataaaa tacagctaag actaaaaaga ttaatgccga gaaataaaat gtattgttta
      781 aattgttggt aaattgtgta attaatccgc caaataatgg ccctatcatt gagccgaatc
      841 cttggatact attaaaaaca ccccaagttt cttcttgttc atctgatttg ataaatcgtg
      901 ccataaaggt attccatgct ggtaataaga tgccatacat tagaccgata gctaaagcga
      961 taatccacaa gatgtgaata ttaacaatca tagatagagt aaaaattaat atcatgtata
     1021 aaataaatcc gcttagaata acaccataca taaagtttct gctgcggtta tctattagtt
     1081 tcgataaaaa tagcatcgaa actgcacagc cgataccacc aataatgatt gcaacagtat
     1141 attcaattgt gcttacgtta ataaccttag tagcatatgt tggtaatata ggaactaggg
     1201 cagcaattgc ggcaccttgt aaaagaatac cagggaacaa caataaatgg cgctttgtca
     1261 catcaacaat ttgtctcaat tgagctttaa ctggacgagt attataattt gttaacttta
     1321 catcgacaaa ataatataat atccatgcaa ttaaaacgac taaagacatc atgaaggcaa
     1381 agcgtgttgg gtgcactttg ataagtagat tcataaaaac catacctacc aataggccta
     1441 acaaccatga aaaataaaca tagcccattt gtttgccacg tttatcttct tcaacactgg
     1501 ataacataat gacccaaata ggactaactg caataccgag catcatagca ctaaatatga
     1561 ttacaaaagg tgatgctgga aaccaaataa ctaaaaataa acttgtaaat gctaaaataa
     1621 atccagtcgt taaaacgatt tttgtgccga attttttcag taaaaatcct ataacaaagt
     1681 ttgtagatgc atcagcaata aaatgtattg aaaatgctag agacgttatt gctacagcaa
     1741 tggatgtaac tgttggcaag aaattaatat agcttaggat atacatgcct ctcgcaaatt
     1801 ccattaaaaa taagataata agcattaaaa tgaaattttt atgattagcg taattattta
     1861 acgaagaatc ttgcatataa aggaaccttt ccataaatct cttgtggttg tgatgaatga
     1921 ccgattaaat caagtaagtc tcgacatatt gtctgtgtag catacttaat tttatcttgt
     1981 tccattgtac taatcatgtt agttaattgc tcattaccgt tagttaaact tgctacaatt
     2041 tttattgctt cttctggagt atcagcgatt ttaccaaaac ctttttcttc aaagtaaagg
     2101 gcattttcaa gctcttgacc aggtgcagga tttaggaaaa tcattggaat acaacgggcg
     2161 aaaccttcag ttattgtgat accaccaggt ttcgtaatca taagttgact tgatgccatc
     2221 cattcattca tgtgtttggt ataacctaga atcaatacat tctcgttaga tttaaactta
     2281 gctgttaaag aacgctttag ctctttgctc ttaccacaaa tcataactac ttgtgcattt
     2341 gcacttttcg ctaatatatc agtaatcatc gtgtcaaaac ctttagatac accaaatgca
     2401 ccagctgaca ttaaaatagt ttgcttatct ggatctaagt tgttgtctat taaccactgc
     2461 ttttgattaa taggcgtttc aaatttgtta tcaataggaa tacctgtcac tttaactgtt
     2521 gaaggatcaa tacctacgtc tatgaagtct tgtttcgttt cttttgttgc cacataatat
     2581 cttgttgaat acggcgtaat ccagttttta tgtaagcgat agtctgtcat cactgtagca
     2641 actggaatat taatgttaaa ttgctcagtt agtaccgaca taactggtgt aggaaacgtt
     2701 aataatatta aatctggctt ttcttttatc aataaattaa ttaacttatt aagtccatag
     2761 tatttgtaaa aacatttgtc tagtttatct gggcggctgt aataaaaccc tttgtacata
     2821 tttctaaaat atttaaagct attgatatac cattttttac aaatagaagt caaaattgga
     2881 tgagcttcca taaataaatc gtgctcaatg acgcttaaat ggtctagatt catatcatta
     2941 agttgattaa cgatactctg tgtaacttgc atatgaccgt taccgaatga gccagtaata
     3001 atcaatatct ttttattttg agtaaccatt aatagccacc ctccgttagt ttgaaaattt
     3061 tatttaagtg taacttattt tacggcatta taaaagaaat aaagacgcaa agtcgttaca
     3121 tttatagcaa ttttaatcta tagatgaatt gatacaaaat aaaacgttat tttataaagc
     3181 aatttattgt tctatgtttt atttgtatat ttaaaattat ccagtataca attatagcat
     3241 atttttggaa acaattatga tattatacca tgttacaaga tggttttaat aatttaagat
     3301 gagccataat tgtaaaacta attcataata ccgtatgttt tatttttaat agtagaaatt
     3361 agaaaatgct gattagtagg atataacagt gaaattataa atttattaac atcaacaaaa
     3421 cgtgtataat aaacatattg tagaaaaagg agcggttcag tttggatgca agtacgttgt
     3481 ttaagaaagt aaaagtaaag cgtgtattgg gttctttaga acaacaaata gatgatatca
     3541 ctactgattc acgtacagcg agagaaggta gcatttttgt cgcttcagtt ggatatactg
     3601 tagacagtca taagttctgt caaaatgtag ctgatcaagg gtgtaagttg gtagtggtca
     3661 ataaagaaca atcattacca gctaacgtaa cacaagtggt tgtgccggac acattaagag
     3721 tagctagtat tctagcacac acattatatg attatccgag tcatcagtta gtgacatttg
     3781 gtgtaacggg tacaaatggt aaaacttcta ttgcgacgat gattcattta attcaaagaa
     3841 agttacaaaa aaatagtgca tatttaggaa ctaatggttt ccaaattaat gaaacaaaga
     3901 caaaaggtgc aaatacgaca ccagaaacag tttctttaac taagaaaatt aaagaagcag
     3961 ttgatgcagg cgctgaatct atgacattag aagtatcaag ccatggctta gtattaggac
     4021 gactgcgagg cgttgaattt gacgttgcaa tattttcaaa tttaacacaa gaccatttag
     4081 attttcatgg cacaatggaa gcatacggac acgcgaagtc tttattgttt agtcaattag
     4141 gtgaagattt gtcgaaagaa aagtatgtcg tgttaaacaa tgacgattca ttttctgagt
     4201 atttaagaac agtgacgcct tatgaagtat ttagttatgg aattgatgag gaagcccaat
     4261 ttatggctaa aaatattcaa gaatctttac aaggtgtcag ctttgatttt gtaacgcctt
     4321 ttggaactta cccagtaaaa tcgccttatg ttggtaagtt taatatttct aatattatgg
     4381 cggcaatgat tgcggtgtgg agtaaaggta catctttaga aacgattatt aaagctgttg
     4441 aaaatttaga acctgttgaa gggcgattag aagttttaga tccttcgtta cctattgatt
     4501 taattatcga ttatgcacat acagctgatg gtatgaacaa attaatcgat gcagtacagc
     4561 cttttgtaaa gcaaaagttg atatttttag ttggtatggc aggcgaacgt gatttaacta
     4621 aaacgcctga aatggggcga gttgcctgtc gtgcagatta tgtcattttc acaccggata
     4681 atccggcaaa tgatgacccg aaaatgttaa cggcagaatt agccaaaggt gcaacacatc
     4741 aaaactatat tgaatttgat gatcgtgcag aagggataaa acatgcaatt gacatagctg
     4801 agcctgggga tactgtcgtt ttagcatcaa aaggaagaga accatatcaa atcatgccag
     4861 ggcatattaa ggtgccacat cgagatgatt taattggcct tgaagcagct tacaaaaagt
     4921 tcggtggtgg ccctgttgat taataaaaga tttattgatg aaggtaaaac tattgatgtt
     4981 tatttattcg aagcattaaa taaccagata atcattgcta taccagattg gttttggtca
     5041 tatcagatgg caatgacatt agatgaagaa acttgttttg aagcaatact catgcaattg
     5101 tttgttttta aagaagagga agaggcagaa tcgattgcat cacaactaac agattggata
     5161 gaaacatata aaaaggagaa agactaatga acttaaagca agaagttgag tctagaaaga
     5221 cttttgcgat tatttcacat cccgatgcag ggaaaacaac gttaactgaa aaactattgt
     5281 acttcagtgg tgctattcgt gaagcgggta cagttaaagg gaagaagact ggtaaatttg
     5341 cgacaagtga ctggatgaaa gttgaacaag agcgtggtat ttctgtaact agttcagtaa
     5401 tgcaatttga ttacgatgat tataaaatca atatcttaga tacaccagga catgaagact
     5461 tttcagaaga tacgtataga acattaatgg cagttgacag tgctgtcatg gtcatagact
     5521 gtgcaaaagg tattgaacca caaacattga agttatttaa agtttgtaaa atgcgtggta
     5581 ttccaatctt tacattcatt aataaattag accgagtagg taaagaacca tttgaattat
     5641 tagatgaaat cgaagagaca ttaaatattg aaacataccc tatgaattgg ccaattggta
     5701 tgggacaaag tttctttggc atcattgata gaaagtctaa aacaattgaa ccatttagag
     5761 atgaagaaaa tatattacat ttgaatgatg attttgagtt ggaagaagat catgcaatta
     5821 caaatgatag tgattttgaa caagcgattg aagaattaat gttggttgaa gaagcgggtg
     5881 aagcctttga taatgacgcg ctgttgagtg gagacttaac acctgtattt ttcggttcag
     5941 ctttagctaa ctttggtgta caaaatttct taaatgcata tgttgatttt gcgccaatgc
     6001 caaatgcgag acaaacaaaa gaagacgttg aagtaagccc gtttgatgat tcattttcag
     6061 gatttatctt taaaattcaa gccaacatgg accctaaaca ccgtgataga attgccttta
     6121 tgcgtgtcgt tagtggtgca tttgaacgtg gtatggatgt tactttgcaa cgtactaata
     6181 aaaagcaaaa gatcacacgt tcaacgtcat ttatggcaga cgataaagaa actgtgaatc
     6241 atgctgtagc aggcgatatc attggactat atgatactgg taattatcaa attggagata
     6301 ctttagttgg tggaaaacaa acctacagtt tccaagattt accacaattt acgccagaaa
     6361 tttttatgaa agtttctgct aaaaacgtca tgaaacagaa gcatttccat aaaggtattg
     6421 aacaattagt acaagaaggt gcgattcaat actataaaac attacacaca aaccaaatta
     6481 ttttaggtgc tgttggtcag ttacaatttg aagttttcga acatagaatg aaaaacgaat
     6541 ataatgttga tgttgttatg gagccagtag gccgtaaaat tgcacgttgg attgaaaatg
     6601 aagaccaaat tacagataag atgaacacat caagatcgat tttagtgaaa gatagatatg
     6661 acgatttagt attcttattt gaaaatgaat ttgcaacaag atggtttgaa gagaaattcc
     6721 ctgaaattaa attgtatagt ttactttaac agctcaattg tataatcgaa tttgttacat
     6781 taaaaataat tgtttcgttg aagaaaaata aattgtatat tttaaaagaa aaaggtatac
     6841 tatgatgtat caaatgaata acctatggca ttttgtcaga ggggagtaac ttaagaatca
     6901 tgaccgtata aatgattcga cactttatcg tcattacgaa gatatcttcc ggtaaagtgg
     6961 gcaatttaaa ttgcttagtg agacctttgc tatttattta gcataggtct ttttgtttgt
     7021 acttaactta tttatttaaa ggagttgtac atgttaatgg atccaagttt gatcttacct
     7081 tatttatggg tacttgtcgt tttagtattt ttagaaggct tattagcagc agataacgcg
     7141 attgttatgg ctgtaatggt taagcactta ccacccgaac aacgtaaaaa agctttgttt
     7201 tacggtttgt taggtgcatt tgtatttaga tttttagcat tattcttaat tagtattatc
     7261 gcgaactttt ggtttattca agctgcagga gcggtttact taatttatat gtcaatcaaa
     7321 aatctgtggc agttctttaa acacccagaa attgaaagtc ctgaagctgg agatgatcat
     7381 cattatgatg aatctggtga agagattaaa gcaagtaaca aatcattctg gggaactgtg
     7441 ttgaaaatag aatttgcaga tatcgcattt gccattgatt ctatgcttgc tgctttagct
     7501 attgctgtaa cacttcctaa agttggtatt cactttggtg gtatggactt aggtcagttc
     7561 gtagtcatgt tcctaggtgg aatgattggt gttattctaa tgcgttatgc agcaacatgg
     7621 tttgtagagc tattaaacaa atatccagga cttgaaggtg cagccttcgc gatcgttggt
     7681 tgggtaggtg ttaaattagt tgtcatggta ttagcgcacc cagacatcgc tgtattgcct
     7741 gagcacttcc cacatggcgt attatggcaa tctattttct ggacagtact aattggatta
     7801 gtaattatcg gttggttagg ttcagttgtt aaaaataaaa aatcgcataa ataattgatg
     7861 tgaagcggac aatcttaatt tagtttaagg ttgtcctttt tcatttaatt gagtgattta
     7921 tgaaaaatgg attttgaaga atgtgaatca aaagatgcga tatagtatta agaaaatgtg
     7981 ccttttatat ttagcatttt ttcaatagaa attatataga ttttaaagca aattaggtgt
     8041 taatgtgtca taatgataag tgattttatt gaatggagtg gacattagtg gatattggta
     8101 aaaaacatgt aattcctaaa agtcagtacc gacgtaagcg tcgtgaattc ttccacaacg
     8161 aagacagaga agaaaattta aatcaacatc aagataaaca aaatatagat aatacaacat
     8221 caaaaaaagc agataagcaa atacataaag attcaattga taagcacgaa cgttttaaaa
     8281 atagtttatc atcgcattta gaacagagaa accgtgatgt taatgagaat aaagctgaag
     8341 aaagtaaaag taatcaggat agtaagtcag catataacag agatcattat ttaacagacg
     8401 atgtatctaa aaaacaaaat tcattagatt cagtggacca agatacagtg aaatcaaaat
     8461 attatgagca aaattctgaa gcgactttat caactaaatc aaccgataaa gtagaatcaa
     8521 ctgaaatgag aaagctaagt tcagataaaa acaaagttgg tcatgaagag caacatgtac
     8581 tttctaaacc ttcagaacat gataaagaga ctagaattga ttctgagtct tcaagaactg
     8641 attcagacag ctcgatgcag acagagaaaa taaaaaaaga cagttcagat ggaaataaaa
     8701 gtagtaatct gaaatctgaa gtaatatcag acaaatcaaa tacagtacca aaattgtcgg
     8761 aatctgatga tgaagtaaat aatcagaagc cattaacttt accggaagaa cagaaattga
     8821 aaagacagca aagtcaaaat gagcaaacaa aaacctatac atatggtgat agcgaacaaa
     8881 atgacaagtc taatcatgaa aatgatttaa gtcatcatat accatcgata agtgatgata
     8941 aagataacgt catgagagaa aatcatattg ttgacgataa tcctgataat gatatcaata
     9001 caccatcatt atcaaaaaca gatgacgatc gaaaacttga tgaaaaaatt catgttgaag
     9061 ataaacataa acaaaatgca gactcgtctg aaacggtggg atatcaaagt cagtcaactg
     9121 catctcatcg tagcactgaa aaaagaaata tttctattaa tgaccatgat aaattaaacg
     9181 gtcaaaaaac aaatacaaag acatcggcaa ataataatca aaaaaaggct acatcaaaat
     9241 tgaacaaagg gcgcgctacg aataataatt atagtgacat tttgaaaaag ttttggatga
     9301 tgtattggcc taaattagtt attctaatgg gtattattat tctaattgtt attttgaatg
     9361 ccatttttaa taatgtgaac aaaaatgatc gcatgaatga taataatgat gcagatgctc
     9421 aaaaatatac gacaacgatg aaaaatgcca ataacacagt taaatcggtc gttacagttg
     9481 aaaatgaaac atcaaaagat tcatcattac ctaaagataa agcatctcaa gacgaagtgg
     9541 gatcaggtgt tgtatataaa aaatctggag atacgttata tattgttacg aatgcacacg
     9601 ttgtcggtga taaagaaaat caaaaaataa ctttctcgaa taataaaagt gttgttggga
     9661 aagtgcttgg taaagataaa tggtcagatt tagctgttgt taaagcaact tcttcagaca
     9721 gttcagtgaa agagatagct attggagatt caaataattt agtgttagga gagccaatat
     9781 tagtcgtagg taatccactt ggtgtagact ttaaaggcac tgtgacagaa ggtattattt
     9841 caggtctgaa cagaaatgtt cctattgatt tcgataaaga taataaatat gatatgttga
     9901 tgaaagcttt ccaaattgat gcatcagtaa atccaggtaa ctcgggtggt gctgtcgtca
     9961 atagagaagg aaaattaata ggtgtagttg cagctaaaat tagtatgcca aacgttgaaa
    10021 atatgtcatt tgcaatacct gttaatgaag tacaaaagat tgtaaaagat ttagaaacaa
    10081 aaggtaaaat tgactatccc gatgtaggtg ttaaaatgaa gaatattgcc agtctaaata
    10141 gttttgaaag acaagcagtt aaattgccag gaaaagttaa gaacggtgtt gttgtagatc
    10201 aagttgacaa caatggttta gcagatcaat ctggtctgaa aaaaggtgat gtaattactg
    10261 aattagatgg caaactttta gaagatgatt tacgctttag gcagattata tttagtcata
    10321 aagatgactt gaaatcaatt acagcgaaga tttatagaga tggtaaagag aaagaaatta
    10381 atattaaact aaaataatgt tgaggtgaac aacgtgtcaa tttttagcca gtttttaaaa
    10441 agatcaagcc ctcaacaagg tattgtattg tactatatcg tcgcaattgt cattgcattt
    10501 ttattattaa acttaccgta tgttcataaa ccaggtgtag aagtaaatcc aattgacaca
    10561 ttatttgttg ccgtatccgg aattagtgtt actggattgt ctccgataag tattgtcgat
    10621 acctattcta catttggaca attaattatc ctcgtgatat taaatattgg tggaattggc
    10681 gtcatggcaa ttggtacgat gttatgggtg gtactaggta aacatattgg aattagagaa
    10741 cgtcagttaa ttatgttaga taataacaaa aacacaatga gtggtaccgt caaattgatt
    10801 attgatattg taaaatcaat atttgtaatc gaactcgtag gagccatgtt attagcattt
    10861 tacttttatc gagataatcc agatttaaaa tatgcaatca tgcaaggtgt ttttgtttct
    10921 atttctgcca ctaccaatgg tggattagat attacaggta agtcattaat tccttatgca
    10981 catgattatt ttgtacaagc gatagttata tttttaataa ttttaggatc aatcggcttc
    11041 ccagtattat tagaagttaa agcttatatt caaaataggg ttactaattt tagattttca
    11101 ttatttacta aaattacgac atcaacatat ttattcctat ttattgttgg ggtattagcc
    11161 attctattat ttgaacataa ccatgcgttc aaaggtttaa gttggcatca atcgttattc
    11221 tattcgctgt ttcaatcagc gactacaaga agtgcgggtc ttcaaacaat tgatgtgaca
    11281 acactaagtg accccactaa tattatcatg ggtattttaa tgtttatagg atcttcgcca
    11341 agttcggttg gtggcggtat tcgtacaaca actttcgcta ttttaatttt gtttttaatt
    11401 aactttagta ataatgccga taaaacatcc attaaagttt acaatagaga agtacacatt
    11461 atggatattc aacgttcatt tgcagtattt acaatggcga caattttaac atttttagga
    11521 atgctaatta tatcagctac tgaaaatggt aagcttacat ttttacaagt attttttgaa
    11581 gtcatgtctg catttggaac ttgtggacta tcgcttggtg tcacaagtga tattagtgat
    11641 atttctaagg tcgtactaat gatactcatg tttataggac gtgttggctt aatatcattt
    11701 atcattatga tagcaggacg tcgagaacca gataaattcc attatccaaa agaacgtatt
    11761 caaataggat aatataatag caatctaagt ttagttaatg tagattttaa ctggaactta
    11821 gattgctttt ttagtttgta tttttaactt attttataag acgattggtt tcgaaaatgg
    11881 taaactagta acaatgagag gtgtaacatg atggaaaaaa atgaaaacat taatgtagag
    11941 attttaacta cgtcagatat gcatagtcat ttcttaaatg gtgattatgg ttcaaatatt
    12001 tatagagctg gtacttatgt taaccaagta agagcacaaa atcatcgcgt cattttatta
    12061 gatagtggcg gaagtttagc tggctcgtta gcggcctatt attatgctat tgttgcacct
    12121 tataaacgac atccaatgat aaagttaatg aacagaatgc attatgatgc tagcggtgtg
    12181 agtccaagtg aattcaagtt tggtttatca tttttaactc gttcaattgc tttggcacgt
    12241 tttccatggt tatcagcaaa tattgaatac aatgttacta aggagcctta tttttcaact
    12301 ccatattgta ttaaacattt tggtgactta aaaattgcta tcgtaggcgt cacagcagat
    12361 ggtttaatgg aaaatgagta ttctgaaatg gagcaagatg tatctattga aaagacatta
    12421 gtggcatcaa aacgttggat tagatatatc catgaagttg aagagccaga ttttttgatt
    12481 gtaatttatc atggtggatt gaataaaatt agtaatagta cgaaaaataa aaaggcaagt
    12541 tcgaatgaag ctgaaaaatt aatggaagaa ctcggtgtta tagatttaat gattacagct
    12601 catcagcatc aaacaatagt aggtcaagat catgaaacgt attatgttca ggctggtcaa
    12661 gatgccaaag agcttgtaca tctttcgatt aattttaaaa agagaacaac aacttatgat
    12721 gttgaaagca ttgattctaa agtgattgac ttaaatgagt atgaagagga tcaagaatta
    12781 ttagatttaa cattctatga tagaaaagca gtggcttatt ggtcacagga aatcataagt
    12841 gataaaggtt tgatgttatc agtaaatggg ttacaagatt tagtctgtca aacacatcca
    12901 ttttcgcaat tattacatga tgcaattcac cttgcatttg ataatgatat aacatgtgtc
    12961 cacgtgccta tgaacggaga gaaggggttg agtggacaga ttcgaaatga agatttgtat
    13021 catgcatacc catatccaga taagccaatg gatatgacaa ttagtggtca aaatatcaaa
    13081 gatatattgg agtatagtta ttcacattta gattttgtta acgagcaatt aagcttaaca
    13141 attattgatg aaacgttatg tacaatgtgg caaggattca attatgagat tgatatgaat
    13201 caagaacctg ggcaacgagt aatgttagat caaattgatt tgactaagag ttatagagtt
    13261 acaatgactg actattgtta tcgtaactac aagaattatt taaaaaatgc tattatacat
    13321 gaatcatacg atgaaacaat gagtacatta attgcagaga agttaagaga tccgaattat
    13381 cgtatttcat gtagtgataa ttttatagtt aaaaacaggt aaatattgct atgtaataaa
    13441 ttaattataa gttcaacaag tataaaaacc tttcttagag aagattatct aggaaaggtt
    13501 tttagaattt taaaattgat ataaaagaac agtgaaattc tcattcatta tatataagta
    13561 aacaaaaaag ctaaaatggt atataccatt ttagcaagta acggaaacgg agggattcga
    13621 accctcgcgc cgctttcgcg acctacaccc ttagcagggg cgcctcttca gccaacttga
    13681 gtacgtttcc aatggctcca caggtaggac tcgaacctac gaccgatcgg ttaacagccg
    13741 atagctctac cactgagcta ctgtggatta atttgtatgt gtttgaatat caagcacaaa
    13801 tattattata acaagattaa gttaatttgc aatagatacc ttaaaaaaat atgcagaaca
    13861 tttacactat atttataaaa cattccgcat attttaaaaa taaacaattt atttatcttc
    13921 aaataaagaa tagcgtgtca atgcttcaaa cacattcaat gtggctttat tataatctat
    13981 tggttggtcg ggattttttg atatcatgcg agattgttta tctaccatgt aatagtaaaa
    14041 aatggaattg ttatattgat gccataaact gtggtatgaa acatgaagaa tgattgattc
    14101 attattaata aatgtcactt tacatttacg attttttaat tctttaatac tttcgatata
    14161 atgcatattt aaccaaatat tttcattttg tctgtcagaa tgtgttggga aaaaataagt
    14221 tggaaataat ggtgttagta aaataggtgg tttactagaa atgccagtaa tgcggtttgt
    14281 ctctgctttc ttgccaagat aagtattacc ataaaatttg caagagcgtt caatgatttt
    14341 ctggacttta aaaggatttt gaatacgcgt tttgtcaaac cgaattattt cactaccgtt
    14401 tctttgatcg tcatcatcaa atgctggtcg aataaccatg tctcctttgc gtatcacata
    14461 aatattttga gaatacataa gcaaaacctc gctttattaa gttttaaacc attaatataa
    14521 aaatacaatt aatgatataa agatatttta taataaaatt ctgagttgtc aagttattat
    14581 tttggtgaaa atgtacaata atttttaact aatagtcaga atattcattg atttcaaaat
    14641 tagaaaaatg tatacttatt ataaattaaa gaaaggtagt ggtttctatg aaatacaata
    14701 ctaatgttaa acatacaact ttagaagcgt ttgtcacaac tgtcaatgat ttgggtattg
    14761 aattaattat caatgaagca cttcgagagg taagaaaacg acagctcata gaacttatag
    14821 atgacgcact cgtcaataaa gatgaagcag catttaatca atatacggca gaatacaaaa
    14881 atttggaggc atttctcggt gaataacatt gattcgaatt ccataacgca atcataaaag
    14941 gactattctt aacatctcat acatgtaaga atagtccttt aatcgtttat gacattaatc
    15001 taattaattc atgtctatct atgtcaccga aataatgata taaatcatat tctgatagtg
    15061 cttcttctat gtgctcaaag tcgtgtagac agccaactaa tgcattttca agatcagtga
    15121 catctccgac accaaagaaa tcaccgaata tttttgcatg ttcgatttta cctcgtttaa
    15181 catcaaactt aatttgtaca aatccttttt caaatttttc ctcgcgttca aagttatatt
    15241 tagggtttct accataattc cattcccaag ttctatattt gtcgttactt aacttttcaa
    15301 tattttccca atcttcatcc gttaatttat attcttctac ttcagtttct ccaaagatag
    15361 ttttcaagat gattttttta aattcttcaa tttctaaagg gtcatttaaa aattcttgaa
    15421 tgtttgctac ccgtttacga acagatttaa taccttttga tttaatctta gctggattaa
    15481 cttttagtgc attctgaact tcgtctaaat cactatttaa catcaacgta ccatgactaa
    15541 acattctatt tttaacttta accatagcat tccctgagat tttcgcttgt ccaacttgaa
    15601 tatcgttacg accagttaat tcagcattta cgcctaaaga ttgtaatgct tgaacaattg
    15661 gttcagtgaa tttttggaaa ttgtggaaac tgttaccatc atcatctgtt ataaaactaa
    15721 agtttaaatt gccagtatca tgataaacag cgccaccacc agaaattctt cttactacat
    15781 cgatgttgtg agcatcgata tatgtctgat ttacttcctc tatcgtattt tgattctttc
    15841 caacaatgat agatggtcta tttatgtaaa ataaaaagta actttcttct gctggtaaat
    15901 tttttaaaac atattcttcc attgctaagt ttaaagttgg atctgtaata ttattattac
    15961 taatgaattt cattacaatc tctcccttat atctatatat attctctact tatttatgcc
    16021 ttaactttgc tcaaaatgcc aattatatac tcacaaatca ttctaaatat cggcttaaag
    16081 ttacatttta ttatttttga ttgttaatag tttatttgtt aagtgaattt tagtacaata
    16141 taatttagtt atgatttaag gaggacttaa aatgactgtt gcagaagtgg gtaacattgt
    16201 tgagtttatg gatggattaa gaggtcgtgt tgaaaaaatc aacgataact ctgttattgt
    16261 tgacttaaca attatggaaa attttaatga ccttgattta ccggaaaaaa ctgttatcaa
    16321 tcataaacga tataagattg ttgaataaga aggtaagtta taatgaataa aatctcgaag
    16381 gctttaactt ggtttattat aagtttcatt atatttcatc tcatattatt tattatgtgg
    16441 ggcgaacacc aagaatactg gtatttatat acaggtataa tgctaattgc tggtatcagt
    16501 tatgtatttt atcaaagaga tattgaatct aagcggttgc ttacatcaat tggtgttggt
    16561 attattacgg caattatttt aattatgctt caacttttat tctcacttat aacttctaat
    16621 ttaagttata gttcattaat taaagaatta gcaagaacag gtgtcaattg gaagtggcaa
    16681 atgttagtaa ctttactttt tgtcattcca tgtcacgagt tatatatgag aactgtttta
    16741 caaaaagaat taacacattt ttcaataccg aaatgggtag ctatattaat aactgcaatt
    16801 tgttctagtt cattatttat ttatttagat aattggtgga tagtaacatt tatatttgtt
    16861 gcacaagtga tattatcatt aagttatgag tatactcgac gtatagcaac aacatccgtt
    16921 gcacaaattg tagctattat attgttatta atttttaatg cttaaataag cttttcttag
    16981 acgtatgtgt tctaagaaaa gcttttttgc ttaagaatag caatacgaag attcgaggca
    17041 tgcttcatta tagtggtaat attttttaac agcaagcggt tattattgag attaggtttt
    17101 agtcagtttt tattactaaa tattttataa aaatggtata atatttttga gcaagttgaa
    17161 ttgatggtgg ctatctgagt aaaggggggt ggtgcctatg gcattactta acacttttga
    17221 aaggaaaagc ctattgtgat atctattgca aacgcattac atttaatgtt aagtttcggt
    17281 atgtttatcg tcactttcat tggtatagta gtagcaataa taaatttaag caataaaaaa
    17341 taaccatcat ttcaaaactt tgacatagaa tgatggttaa agaaaatctc atttagtcac
    17401 cgtcttttta acgggctcat taggcgacat gttcctgcat gtcgtctttt tacattttta
    17461 tagtaacata tcttttattt aattgtctat ttttattaga tgtgtaacaa aattttgaag
    17521 tattttccgt atattgcctg ctaaacatgt cagatttgaa aggaaaatca tactgtggta
    17581 gaaatcaatt atgattatat ggtagtactt ataaataagt gatttctatt gaatatgtaa
    17641 tgaagttcat atattaatat aactttcatt gatatagaag tcgcaatgat taatttaaac
    17701 aataaaaaac aaccatcatt tccaaacttt gacaacgaat gatggttaaa gaaaatctca
    17761 tttagtcacc gtctttttaa cgggctcaat aggcgacatg ttcctgcata tcgtctattt
    17821 atacactgcg caatttaata ataatacaat tatactttga tgtccattta acataaagtg
    17881 gttgaaattt gcagcaaata aattaacctt gactattttc agaaaattct gtataaataa
    17941 cgaaaaagag atatggatta aatatgaaga ggtgttttta tagaagaata aaatttttgg
    18001 cactatatta tatttaactt tagcacttgg attatcaaca gcagcttatg catctacaga
    18061 atacgcagaa ggaggcactt ggagtcatgg tgtcggcagt aagtatgttt ggtcgtatta
    18121 ttatcatggt cataaaggac atggtgcaac agctattgga aaatatagat catttagtgg
    18181 ttatacaaga gctggtgtaa aagcaaaagc atcagctact aaacataatt ggtgggtcaa
    18241 tagagcgtat tataacattt attaaatact aaaaaatcat agaaaataaa caacgcaaga
    18301 ggtacaacat gaaatggttc aaattaatac ttgatgttac aatcttcatt ctgattgcta
    18361 tattgttatt tgtttataca tataaagaaa acgaagaaat attgcctgat actaaatacc
    18421 ctatagcggt aactgactgg aataaaaaat atagtaaaaa tgagatttat aaacgtatag
    18481 atcaattcgc taaaaatgag aacgtagcaa tctataaatc aacttcaaat tatacaaaca
    18541 aaaacgtaga taaagatata tatgtattta ataaatcaaa agcagcaact atcactcctt
    18601 ttaatgctaa atataacatt cattatttaa gtgacgacga agtattaaaa aaagatatca
    18661 aagggagtta ttttgtaaaa gacaaaaatt ttgacgtgtc taaattcata aattttttaa
    18721 aagaatatgg tgttactgct gaatcataca aaatagatca tatgatgatt gccgttggtg
    18781 tcattaaaca aatgaatata gtagttccat tatcctcact tttaatcgtt tattttattt
    18841 attatatttt cgaaaagaac attaatttca aagcgtatgc gattaagtat ttaaatggtt
    18901 ttacattaag aaaaataatt ttcgaaaatt tttcaaagaa atgcacgtat tgggtaacgt
    18961 taatcataac tcaaattctt ttaactacaa gtgtactttg gatattaaat tacacgggta
    19021 atttagattt attcatatta agaatagttc tgctttcatg tcttttcatt ttaacgatta
    19081 gtgttattaa tttatggact ttcttaatgt tactaaattt aaatattgca aatatgatta
    19141 aaggtaagca gcactttaaa acaattcgtt ttattaatac agtttgtaaa agtattctct
    19201 tagtactaat agctaatgta atgatagaaa atactagtgt tattaaagat ttgaataaaa
    19261 taaaagaaac tgagaaatat tggaatgtat tagatgatta ttacacgatt gaatttgcac
    19321 cttatcacga aacaaaacaa agtttgattg ataatatggt gcgatcagaa caattagtaa
    19381 aggctagtga agcagaaaat aatgcgattt tattcaaacc aaagggtgac tccgttgaca
    19441 atgacaactt ttcgcctgat gaggggaatg taatattagt aaataatcag ttctggtcga
    19501 tttattacaa gcagtttcaa cctgatattc cgataaaaaa tcaaaaaaat aatgtcgaag
    19561 taattattcc acaaaagttt catgcaatgc gtaatgaaat caatcaagca tatcattcat
    19621 ggtttgaatt tgtacaaaat aaaaataata aagagaataa gttatctata cagtttatca
    19681 acaaaaatga ttgtcgaatt ttttcatttg atgcacgaga tagtcgccat ttgtcattta
    19741 tagaggcgcc aatcattgtg aatgttcagg catcagattt atcgaatgat ttttattatg
    19801 ccatgatcag tcaaggcggg tatttattca aaaattatga cgcgctagta aaaaatattg
    19861 aaaagtatca tcttgatggg gaaatcagtg gaataaccaa ttataaagat agcgtgatgg
    19921 aaatgtatca tgaaaacaat ttgaaattaa cagtactcaa cttttcacaa atcattatcg
    19981 caatcatttt aataattatt attttatttg atgtgaaata ttattttgaa cagcatcgaa
    20041 aattactcgt aatcaaaaag ctatatggtt attcaacatt aagagccaat taccaatact
    20101 tattaataaa taatatagtt gttattttta ttggaatatt gacgaatgta attttacatt
    20161 ctcactatat aatgatgtta tttgcaacga ttcttgtcgt tcaaatatta ttgcagattt
    20221 gcagcctata ctatcatggc cgacgtttta atgaagttat caaggagttt taactatgaa
    20281 atttatcatt gcaatattat taggattaat attatccatt actattgctt ttacaatcat
    20341 acatcatcct atacttgatc gttttaatcc tttcttaaaa acggagtata gttatgccaa
    20401 agtgccaaaa ggtacgcaac aatatgttaa tattacggct tatagtgaaa gaggggaaaa
    20461 gcttgattat aaattaacat ttaatggatt ttcacctagt agaacgtatg tcgaaataaa
    20521 gcataaaggg caatatgtca tatcgatcac atatgttgaa aaagaggata caccaaaaga
    20581 ggtaagacaa gaatgataga attaaaagat ttaaccatac aaaaaggtaa tacacatatt
    20641 ttgaagaaac ttaatttgaa atttcaatgt ggaaaatcat atgcacttat tggtaaaagt
    20701 ggatgcggta aatctacatt attaaatact attgctggac ttgaaaaaac aggaaaacaa
    20761 tatgtctatt ttaatggtca attagaacaa tttaaatcta atttttacag agataaatta
    20821 ggatatttat ttcaaaatta tggattaatc gataatttga cagtaaatga aaatttagat
    20881 attggattag catataaaaa aataagtaag aaagaaaaag aacaaataaa gatacgttat
    20941 atagaacagt ttggtctgtc aaacagttta aaaagaaaag ttcatacgct aagtggaggt
    21001 gaacaacaac gtgtcgcttt aattagaatg atgttaaaag atccgattgt tatgttagct
    21061 gatgaaccaa cgggtgcgtt agatcctaaa acaggacaga tgattattca atcattattt
    21121 ggtttggtcg atgaaaataa agtgctgatt ttagcaacac atgatatggc tattgcaaat
    21181 caatgtgacg aaataataga tttagaacag tatagaaaag tagcatctat gtgatatcca
    21241 aaagataaaa ttaaaagcca atcaatggga aatgttgact tcaacactcc gtttgattgg
    21301 cttttatact gtgttactaa gttatgaaag taacaacggc aaactgaaaa aaagcaatat
    21361 aataatattt aatacgatta gtaataaatg taattgattt ttcgttttaa tatagcaaaa
    21421 aacaatgact aaagataaca acaatatcat gattgcacct gggaagaacc ataatgctga
    21481 agctgtgtac caaatcattg gtaacgccat aataataaaa gatataagac aagaaaggat
    21541 accaataata ttataagttc tagtcaaaat tacacctcca attttcactt taaaatgaca
    21601 tgcgtaaaac aatcatttta aatacattat acaataaaat taaattgtgc ggtagaagaa
    21661 tttgaaatta aattgaattg tttttggtag ttggtatatt tataacactc tgcaaaagaa
    21721 tgtagcaaaa ttagataaat tagattattt a
//
LOCUS       DALAGD010000050        21518 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.050, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000050 DALAGD010000000
VERSION     DALAGD010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 21518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21518
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.050"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            198..1199
                     /gene="argF"
                     /locus_tag="O2701_001493"
     CDS             198..1199
                     /gene="argF"
                     /locus_tag="O2701_001493"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCZ4293389.1"
                     /translation="MKNLRNRSFLTLLDFSRQEVEFLLTLSEDLKRAKYIGTEKPMLK
                     NKNIALLFEKDSTRTRCAFEVAAHDQGANVTYLGPTGSQMGKKETTKDTARVLGGMYD
                     GIEYRGFSQRTVETLAEYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKDYADINFTY
                     VGDGRNNVANALMQGAAIMGMNFHLVCPKELNPTDELLNRCKNIAAENGGNILITDDI
                     DQGVKGSDVIYTDVWVSMGEPDEVWKERLELLKPYQVNKEIMDKTGNPNVIFEHCLPS
                     FHNADTKIGQQIFEKYGIREMEVTDEVFESKASVVFQEAENRMHTIKAVMVATLGEF"
     gene            1222..2154
                     /gene="arcC"
                     /locus_tag="O2701_001494"
     CDS             1222..2154
                     /gene="arcC"
                     /locus_tag="O2701_001494"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCZ4293390.1"
                     /translation="MAKIVVALGGNALGKSPQEQLELVKNTAKSLVGLITKGHEIVIS
                     HGNGPQVGSINLGLNYAAEHNQGPAFPFAECGAMSQAYIGYQLQESLQNELHSIGMDK
                     QVVTLVTQVEVDENDPAFNNPSKPIGLFYNKEEAEQIQKEKGFIFVEDAGRGYRRVVP
                     SPQPISIIELESIKTLIKNDTLVIAAGGGGIPVIREQHDGFKGIDAVIDKDKTSALLG
                     ANIQCDQLIILTAIDYVYINFNTENQQPLKTTNVDELKRYIDENQFAKGSMLPKIEAA
                     ISFIENNPKGSVLITSLNELDAALEGKVGTVIKK"
     gene            2326..3882
                     /locus_tag="O2701_001495"
     CDS             2326..3882
                     /locus_tag="O2701_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HCZ4293391.1"
                     /translation="MENTINESEKKKRFKLKMPGAFMILFILTVVAVIATWVIPAGAY
                     SKLSYEPSSQELKIVNPHNQVKKVPGTQQELDKMGVKIKIEQFKSGAINKPVSIPNTY
                     ERLKQHPAGPEQITSSMVEGTIEAVDIMVFILVLGGLIGVVQASGSFESGLLALTKKT
                     KGHEFMLIVFVSILMIIGGTLCGIEEEAVAFYPILVPIFIALGYDSIVSVGAIFLASS
                     VGSTFSTINPFSVVIASNAAGTTFTDGLYWRIGACIVGAIFVISYLYWYCKKIKNDPK
                     ASYSYEDKDAFEQQWSVLKDDDSAHFTLRKKIILTLFVLPFPIMVWGVMTQGWWFPVM
                     ASAFLIFTIIIMFIAGTGKSGLGEKGTVDAFVNGASSLVGVSLIIGLARGINLVLNEG
                     MISDTILHFSSSLVQHMSGPLFIIVLLFIFFCLGFIVPSSSGLAVLSMPIFAPLADTV
                     GIPRFVIVTTYQFGQYAMLFLAPTGLVMATLQMLNMRYSHWFRFVWPVVAFVLIFGGG
                     VLITQVLIYS"
     gene            4190..4417
                     /locus_tag="O2701_001496"
     CDS             4190..4417
                     /locus_tag="O2701_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293392.1"
                     /translation="MTHLTKVLDTLTGICVVLLFSKYFVAYANMVFDWNLRWYLLENI
                     PHLPIILFILMFIFGVPSEMIKDRQRKNNGV"
     gene            complement(4722..5669)
                     /locus_tag="O2701_001497"
     CDS             complement(4722..5669)
                     /locus_tag="O2701_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HCZ4293393.1"
                     /translation="MRLQKAPLVTSGLVLGLLGLGNLLKDLSLTLNAVCGIFAFLIWI
                     HLLCTMIKYFNNVKEQLNSPLVSSVFTTFFMSGFLGTTYLNTFFSNITFINSLITPIW
                     ILCLVGIMTHMIIFSIKYLKDFSLKNVYPSWTVLFIGIAIAGLTAPVSGYFFIGQLTV
                     IYGFVATCIVLPIVFKRLKAFPLQTSIKPNTSTICAPFSLVAAAYVIAFPKANAFIVI
                     IFLLLAQIFYFYIIIQLPKLLKEPFSPVFSAFTFPLVISATALKNSLPVLMFPDIWKG
                     LLFIEVLLATVIVLRVFIGYLHFFLKKEKQDKFLRNASQ"
     gene            5918..6106
                     /locus_tag="O2701_001498"
     CDS             5918..6106
                     /locus_tag="O2701_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCZ4293394.1"
                     /translation="MRNQIQKLLDSDLSSLHISKQTGVPQSTIHRMRKNERSLDNMSL
                     KNAELLYKFANSIFSNEN"
     gene            complement(6581..6654)
                     /locus_tag="O2701_001499"
     tRNA            complement(6581..6654)
                     /locus_tag="O2701_001499"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6618..6620),aa:Arg,seq:tct)
     gene            7018..7152
                     /locus_tag="O2701_001500"
     CDS             7018..7152
                     /locus_tag="O2701_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HCZ4293395.1"
                     /translation="MEGLFNAIKDTVTAAINNDGAKLGTSIVSIVENGVGLLGKLFGF
                     "
     gene            7209..7343
                     /locus_tag="O2701_001501"
     CDS             7209..7343
                     /locus_tag="O2701_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HCZ4293396.1"
                     /translation="MTGLAEAIANTVQAAQQHDSVKLGTSIVDIVANGVGLLGKLFGF
                     "
     gene            7467..8162
                     /locus_tag="O2701_001502"
     CDS             7467..8162
                     /locus_tag="O2701_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HCZ4293397.1"
                     /translation="MGKLGYKNILIDFDDTIVDFYDAEEWAFHYMANVFNHKATKDDF
                     LTFKKINHQHWEAFQQNKLTKSEVLSERFVNYFKHHQMEVDGHRADVLFRNGLAEAKV
                     KYFDQTLETIVELSKRHDLYIVTNGVTETQKRRLNQTPLHKYIKKIFISEETGYQKPN
                     PEFFNYVFNDIGEDERQHSIIVGDSLTSDILGGINAGIATCWFNFRGFDHNPGIIPDY
                     EINSWKQLNDIVR"
     gene            complement(8271..8711)
                     /locus_tag="O2701_001503"
     CDS             complement(8271..8711)
                     /locus_tag="O2701_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCZ4293398.1"
                     /translation="MSEIKRLEINYKTDELFENFRAFGNKDLYMVNELNGQMIDASSD
                     SPFYGIFVGDQLGARMALLKKGDVEEIYFPDFEDYILLWKLEVLPKYQNRGYASELID
                     FAKSFNMPIKAIGRNDSKDFFLHHGFTDVEAKNIEGHDVLLWKP"
     gene            8906..10519
                     /gene="bshC"
                     /locus_tag="O2701_001504"
     CDS             8906..10519
                     /gene="bshC"
                     /locus_tag="O2701_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HCZ4293399.1"
                     /translation="MDCKVVSLNEKDQFIPKIKSSDPVITGLFQYDAAQQTSFEKRMS
                     KENNGREAALANVIREYMSDLKLSSEQELNIQHLANGSKVVIGGQQAGLFGGPLYTFH
                     KIFSIITLSKELTDTHKQQVVPVFWIAGEDHDFDEVNHTFVYNENHGSLHKVKYHTME
                     MPETTVSRYYPDKAELKQTLKTMFIHMKETVHTQGLLEICDRIIDQYDSWTDMFKALL
                     HETFKAYGVLFIDAQFEPLRKMEAPMFKKILKKHQLLDDAFRATQQRTQNQGLNAMIQ
                     TDTNVHLFLHDENMRQLVSYDGKHFKLNKTDKTYIKEEIINIAENQPELFSNNVVTRP
                     LMEEWLFNTVAFVGGPSEIKYWAELKDVFELFDVEMPIVMPRLRITYLNDRIEKLLSK
                     YNIPLEKVLVDGVEGERSKFIREQASHQFIEKVEGMIEQQRRLNKDLLDEVAGNQNNI
                     NLVNKNNEIHIQQYDYLLKRYLLNIERENDISMKQFREIQETLHPMGGLQERIWNPLQ
                     ILNDFGTDVFKPSTYPPLSYTFDHIIIKP"
     gene            10663..11094
                     /gene="mraZ"
                     /locus_tag="O2701_001505"
     CDS             10663..11094
                     /gene="mraZ"
                     /locus_tag="O2701_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCZ4293400.1"
                     /translation="MFMGEYDHQLDTKGRMIIPSKFRYDLNERFIITRGLDKCLFGYT
                     LDEWQQIEEKMKTLPMTKKDARKFMRMFFSGAVEVELDKQGRINIPQNLRKYANLTKE
                     CTVIGVSNRIEIWDRETWNDFYEESEESFEDIAEDLIDFDF"
     gene            11110..12045
                     /gene="rsmH"
                     /locus_tag="O2701_001506"
     CDS             11110..12045
                     /gene="rsmH"
                     /locus_tag="O2701_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017724209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCZ4293401.1"
                     /translation="MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLND
                     DGRLIAIDQDQTAIDNAKEVLKDHLHKVTFVHSNFRELTQILKDLNIEKVDGIYYDLG
                     VSSPQLDIPERGFSYHHDATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFS
                     KQIARRIEAHREQQPITTTLELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELS
                     AFEDSIEQAIELVKVDGRISVITFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTP
                     KLKRVNRKPITATEEDLDDNNRARSAKLRVAEILK"
     gene            12059..12460
                     /gene="ftsL"
                     /locus_tag="O2701_001507"
     CDS             12059..12460
                     /gene="ftsL"
                     /locus_tag="O2701_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCZ4293402.1"
                     /translation="MAVEKVYQPYDEQVYNSIPKQQPQTKPEKKTVSRKVVVQLTKFE
                     KVLYITLITVIAMLSIYMLSLKMDAYDTRGKIADLDYKIDKQSSENSALQSEIKKNSS
                     YERIYEKAKKQGMSLENDNVKVVRSNGEAKN"
     gene            12441..14675
                     /locus_tag="O2701_001508"
     CDS             12441..14675
                     /locus_tag="O2701_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCZ4293403.1"
                     /translation="MAKQKIKIKKNKIGAVLLVGLFGLLFFILVLRISYIMITGHSNG
                     QDLVMKANEKYLVKNAQQPERGKIYDRNGKVLAEDVERYKLVAVIDKKASANSKKPRH
                     VVDKKETAKKLSTVINMKPEEIEKRLSQKKAFQIEFGRKGTNLTYQDKLKIEKMNLPG
                     ISLLPETERFYPNGNFASHLIGRAQKNPDTGELKGALGVEKIFDSYLSGSKGSLRYIH
                     DIWGYIAPNTKKEKQPKRGDDVHLTIDSNIQVFVEEALDGMVERYQPKDLFAVVMDAK
                     TGEILAYSQRPTFNPETGKDFGKKWANDLYQNTYEPGSTFKSYGLAAAIQEGAFDPDK
                     KYKSGHRDIMGSRISDWNRVGWGEIPMSLGFTYSSNTLMMHLQDLVGADKMKSWYERF
                     GFGKSTKGMFDGEAPGQIGWSNELQQKTSSFGQSTTVTPVQMLQAQSAFFNDGNMLKP
                     WFVNSVENPVSKRQFYKGQKQIAGKPITKDTAEKVEKQLDLVVNSKKSHAANYRIDGY
                     EVEGKTGTAQVAAPNGGGYVKGPNPYFVSFMGDAPKKNPKVIVYAGMSLAQKNDQEAY
                     ELGVSKAFKPIMENTLKYLNVGKSKDDTSNAEYSKVPDVEGQDKQKAIDNVSAKSLEP
                     VTIGSGTQIKAQSIKAGNKVLPHSKVLLLTDGDLTMPDMSGWTKEDVIAFENLTNIKV
                     NLKGSGFVSHQSISKGQKLTEKDKIDVEFSSENVDSNSTNNSDSNSDDKKKSDSKTDK
                     DKSD"
     gene            14967..15932
                     /locus_tag="O2701_001509"
     CDS             14967..15932
                     /locus_tag="O2701_001509"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCZ4293404.1"
                     /translation="MIFVYALLALVITFVLVPVLIPTLKRMKFGQSIREEGPQSHMKK
                     TGTPTMGGLTFLLSIVITSLVAIIFVDQANPIILLLFVTIGFGLIGFIDDYIIVVKKN
                     NQGLTSKQKFLAQIGIAIIFFVLSNVFHLVNFSTSIHIPFTNVAIPLSFAYVIFIVFW
                     QVGFSNAVNLTDGLDGLATGLSIIGFTMYAIMSFVLGETAIGIFCIIMLFALLGFLPY
                     NINPAKVFMGDTGSLALGGIFATISIMLNQELSLIFIGLVFVIETLSVMLQVASFKLT
                     GKRIFKMSPIHHHFELIGWSEWKVVTVFWAVGLISGLIGLWIGVH"
     gene            15934..17283
                     /locus_tag="O2701_001510"
     CDS             15934..17283
                     /locus_tag="O2701_001510"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCZ4293405.1"
                     /translation="MLNYTGLENKNVLVVGLAKSGYEAAKLLSKLGANVTVNDGKDLS
                     QDAHAKDLESMGISVVSGSHPLTLLDNNPIIVKNPGIPYTVSIIDEAVKRGLKILTEV
                     ELSYLISEAPIIAVTGTNGKTTVTSLIGDMFKKSRLTGRLSGNIGYVASKVAQEVKPT
                     DYLVTELSSFQLLGIEKYKPHIAIITNIYSAHLDYHENLENYQNAKKQIYKNQTEEDY
                     LICNYHQRQVIESEELKAKTLYFSTQQEVDGIYIKDGFIVYKGVRIINTEDLVLPGEH
                     NLENILAAVLACILAGVPIKAIIDSLTTFSGIEHRLQYVGTNRTNKYYNDSKATNTLA
                     TQFALNSFNQPIIWLCGGLDRGNEFDELIPYMENVRAMVVFGQTKAKFAKLGNSQGKS
                     VIEANNVEDAVDKVQDIIEPNDVVLLSPACASWDQYSTFEERGEKFIERFRAHLPSY"
     gene            17299..18618
                     /locus_tag="O2701_001511"
     CDS             17299..18618
                     /locus_tag="O2701_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HCZ4293406.1"
                     /translation="MDDKTKNDQQESNEDKDELELFTRNTSKKRRQRKRSKATHFSNQ
                     NKDDTSQQADFDEEIYLINKDFKKEESNDKNNDSASSHANDNNIDDSTDSNIENEDYR
                     YNQEIDDQNESNVISVDNEQPQSAPKEQNSDSIDEETVTKKERKSKVTQLKPLTLEEK
                     RKLRRKRQKRIQYSVITILVLLIAVILIYMFSPLSKIAHVNINGNNHVSTSKINKVLG
                     VKNDSRMYTFSKKNAINDLEENPLIKSVEIHKQLPNTLNVDITENEIIALVKYKGKYL
                     PLLENGKLLKGSNDVKINDAPVMDGFKGTKEDDMIKALSEMTPEVRRYIAEVTYAPSK
                     NKQSRIELFTTDGLQVIGDISTISKKMKYYPQMSQSLSRDSSGKLKTRGYIDLSVGAS
                     FIPYRGNTSSQSESDKNVTKSSQEENQAKEELQSVLNKINKQSSKNN"
     gene            18724..20136
                     /gene="ftsA"
                     /locus_tag="O2701_001512"
     CDS             18724..20136
                     /gene="ftsA"
                     /locus_tag="O2701_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCZ4293407.1"
                     /translation="MEEHYYVSIDIGSSSVKTIVGEKFHNGINVIGTGQTYTSGIKNG
                     LIDDFDIARQAIKDTIKKASIASGVDIKEVFLKLPIIGTEVYDESNEIDFYEDTEING
                     SHIEKVLEGIREKNDVQETEVINVFPIRFIVDKENEVSDPKELIARHSLKVEAGVIAI
                     QKSILINMIKCVEACGVDVLDVYSDAYNYGSILTATEKELGACVIDIGEDVTQVAFYE
                     RGELVDADSIEMAGRDITDDIAQGLNTSYETAEKVKHQYGHAFYDSASDQDIFTVEQV
                     DSDETVQYTQKDLSDFIEARVEEIFFEVFDVLQDLGLTKVNGGFIVTGGSANLLGVKE
                     LLSDMVSEKVRIHTPSQMGIRKPEFSSAISTISSSIAFDELLDYVTINYHDNEETEED
                     VIDVKDKDNESKLGGFDWFKRKTNKKDTHENEVESTDEEIYQSEDNHQEHKQNHEHVQ
                     DKDKDKEESKFKKLMKSLFE"
     gene            20169..21341
                     /gene="ftsZ"
                     /locus_tag="O2701_001513"
     CDS             20169..21341
                     /gene="ftsZ"
                     /locus_tag="O2701_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCZ4293408.1"
                     /translation="MLEFEQGFNHLATLKVIGVGGGGNNAVNRMIDHGMNNVEFIAIN
                     TDGQALNLSKAESKIQIGEKLTRGLGAGANPEIGKKAAEESREQIEDAIQGADMVFVT
                     SGMGGGTGTGAAPVVAKIAKEMGALTVGVVTRPFSFEGRKRQTQAAAGVEAMKAAVDT
                     LIVIPNDRLLDIVDKSTPMMEAFKEADNVLRQGVQGISDLIAVSGEVNLDFADVKTIM
                     SNQGSALMGIGVSSGENRAVEAAKKAISSPLLETSIVGAQGVLMNITGGESLSLFEAQ
                     EAADIVQDAADEDVNMIFGTVINPELQDEIVVTVIATGFDDKPTSHGRKSGSTGFGTS
                     VNTSSNATSKDESFTSNSSNAQATDSVSERTHTTKEDDIPSFIRNREERRSRRTRR"
BASE COUNT         7862 a         2915 c         4067 g         6674 t
ORIGIN      
        1 gaattttcta actacttgaa aaatagttat actttaaatg tagtacttat tttaattatt
       61 tcctactact taaatttaat attaataaaa tgttcattta attattgata aaatattaca
      121 aattttaata gtaggttgtg tttattttgt atgcgcttac aatttaggtg taactaaaat
      181 aaaaggagtt gttattaatg aaaaatttac gaaacagaag ttttttaact ttattagact
      241 tttcacgaca agaggtagaa ttcttattaa cactctccga ggatttaaaa cgtgctaaat
      301 atattggcac tgaaaagcct atgttaaaaa ataaaaatat tgcactgtta tttgaaaaag
      361 attctacaag aacgcgatgt gcatttgaag ttgcagcgca tgatcaaggt gcaaatgtaa
      421 cttatttagg cccaactgga tcacaaatgg gtaaaaaaga aacaactaaa gatactgcac
      481 gtgtgcttgg tggaatgtat gatggcattg aataccgtgg tttttcacaa agaacagtag
      541 aaactttagc tgagtattca ggcgtaccag tgtggaatgg tttaactgat gaagatcatc
      601 ctactcaagt tcttgctgat ttcttaacag caaaagaagt cttaaaaaaa gattatgcag
      661 atattaactt tacatatgtt ggagatggtc gtaataacgt tgcaaatgca ttaatgcaag
      721 gtgctgccat tatgggtatg aacttccatt tagtttgtcc aaaagaatta aatccaacag
      781 atgaattatt aaatcgctgt aaaaatattg ccgctgaaaa tggtggcaac atattaatca
      841 cagatgatat tgaccaaggt gtaaaaggtt cggatgtaat ttacactgat gtttgggtat
      901 caatgggtga acctgatgaa gtatggaaag aacgacttga attattgaaa ccatatcaag
      961 taaataaaga aataatggat aaaactggta atccaaatgt tatttttgag cattgcttac
     1021 catctttcca taatgctgat acgaaaatag gtcaacaaat ttttgaaaaa tatggtattc
     1081 gagaaatgga agttacagat gaagtattcg aaagtaaagc ttcagttgta ttccaagaag
     1141 ctgagaacag aatgcataca atcaaagcag tcatggttgc tacattgggt gaattttaaa
     1201 tgatataagg aagtgaatat gatggcgaaa atagtagtag cattaggtgg taatgcttta
     1261 ggaaaatcac ctcaagaaca actcgagctt gttaaaaata ctgcgaaatc attagtagga
     1321 ttaataacaa aaggacatga gattgttatt agtcatggta atggaccaca ggttggaagc
     1381 attaatttgg gacttaacta tgctgcagaa cataaccaag gtccggcatt tccatttgct
     1441 gaatgtggcg caatgagtca agcttacatc ggctatcaat tacaagaaag cttacaaaat
     1501 gaattgcatt ctattggaat ggataaacaa gtggtaacac tagtgacaca agttgaagtt
     1561 gatgaaaatg atccggcatt taacaatcct tcaaaaccaa ttgggttatt ttacaacaaa
     1621 gaagaagctg aacaaattca aaaagaaaaa ggatttatat ttgttgaaga tgctggaaga
     1681 ggatatagac gcgttgttcc ttcaccacaa cccatctcta ttattgaatt agagagtatt
     1741 aaaacactta ttaaaaatga tacactcgtt attgctgctg gtggtggagg tataccagta
     1801 attagagagc aacatgatgg ttttaaaggt attgatgcag ttatagacaa agataaaaca
     1861 agtgcgttgt tgggtgctaa tattcaatgc gatcaattga ttattttaac agcaattgat
     1921 tatgtatata ttaattttaa cactgaaaac caacagcctt tgaaaacaac aaatgttgat
     1981 gaattaaaac gatatataga cgaaaatcaa tttgcaaaag gaagtatgtt accaaaaatt
     2041 gaagcagcca tatcatttat tgaaaacaat ccaaaaggaa gtgtgcttat aacatcatta
     2101 aatgaattag atgctgcctt agagggtaaa gtaggtactg tgattaaaaa gtaattgaat
     2161 tgaaacgctt ttcaattact atatgtcaaa tgcatgattt ttattattta tgtgcacccc
     2221 ctgaaaataa tgcctctatt ttgatgcggg gtgcactttc ttaatttata tttataaaat
     2281 ctttaaggta gaaatgctag gttaaatgtc gaaggagatg aaaccgtgga aaatacaatt
     2341 aatgaaagtg aaaagaaaaa acgatttaaa ttaaaaatgc caggtgcatt tatgatttta
     2401 ttcattttaa cggttgttgc agttatagca acatgggtta ttcctgctgg tgcatattct
     2461 aaactttctt acgaaccttc atcccaagaa ctaaagatag ttaaccctca taaccaagtg
     2521 aaaaaggttc cgggtacgca acaggaacta gacaaaatgg gggttaaaat taagattgaa
     2581 caatttaaat caggtgcaat taataagccg gtatcaattc cgaatactta tgaaagatta
     2641 aagcaacatc cagctggacc agaacaaata acaagtagca tggttgaagg tacgatagaa
     2701 gcggtcgata tcatggtatt cattcttgta ctagggggac ttattggcgt agttcaagcc
     2761 agtggttctt ttgaatcggg attgttagct ttaacgaaga aaacaaaagg gcatgaattt
     2821 atgctaattg tgtttgtatc aatactaatg attatcggcg ggacattatg tggtattgaa
     2881 gaagaagctg tagcatttta tccgatttta gtccctatat ttatagcgtt aggatacgat
     2941 tctatcgttt cagttggtgc catattcctt gccagctctg tcggtagtac attttcaact
     3001 attaacccgt tctcggttgt aattgcctct aatgccgctg gtacaacttt tacggatggc
     3061 ttgtattgga gaataggtgc ttgtattgtc ggtgcgattt ttgttattag ttatttatat
     3121 tggtactgta aaaaaattaa aaacgatcct aaagcgtcat attcttatga agacaaagat
     3181 gcttttgaac agcaatggtc tgtattaaaa gatgacgata gtgcccattt tactttgcgt
     3241 aagaagataa tccttacatt atttgtacta ccatttccaa ttatggtatg gggagttatg
     3301 acgcaaggtt ggtggttccc agttatggct tcagcatttt taatatttac aattataata
     3361 atgtttattg ctgggacagg taaatctgga ttgggagaaa aaggaactgt agatgcattt
     3421 gtcaatggtg catcaagttt agtaggtgta tctttaatta ttggtttagc tcgaggtatt
     3481 aatttagtgt tgaatgaagg tatgatttca gatacaatct tacacttttc atcatcttta
     3541 gttcaacata tgagtggacc attatttatc atcgtattac tatttatttt cttctgttta
     3601 ggttttatcg tgccatcatc ttctggctta gcggtattat caatgcctat ctttgcacca
     3661 ctagctgata cagtaggtat accaagattc gtcatcgtta cgacatatca atttggtcaa
     3721 tatgcgatgt tattcttagc gccgactgga cttgttatgg ccacactaca aatgttaaac
     3781 atgcgatatt cacattggtt ccgatttgta tggccggtag ttgcttttgt attgattttc
     3841 ggtggcggag tactaattac gcaagtacta atttattcat aatttgaaat gctatattat
     3901 aaaaaatact aatggggttt tatgcatctc gtaggtttgt agaaatacta aactaagcga
     3961 ggtgcattat tatttttgat taagaaaata atgacggtaa tgataacact agtaagtaat
     4021 tgatacaatg ctctatttaa taatgatatt tttaaaattt gtttttaatg taatgttaga
     4081 tctatggtat attatattta tcgtggtaaa tatgtatttg ctgtaaacag tttttgtaac
     4141 acatgataca tatggttgtg aaaatagcat atataaagga tggctataaa tgacacattt
     4201 gacaaaggtt ttagatacac taactggaat atgcgtagta ttattattta gtaaatattt
     4261 tgtggcgtat gcaaatatgg tgtttgattg gaatttaaga tggtatttgc tagaaaacat
     4321 accacatttg ccaattatat tatttattct gatgtttatt ttcggagtac cttctgaaat
     4381 gataaaagat aggcaaagga aaaataacgg tgtttaattt atcgatattt agaggtgata
     4441 aaatttgtca actattacaa agactaaaat aaaaatcttt attaattatt ttattggtat
     4501 attagccttg gcgtttgctg cttatatatt atttaaataa agtttagagt gaaacgtgtt
     4561 tatgaactag acgttctagt atagttacta tagctttagt cagaaggtat cattgataag
     4621 atcatattaa atcaaagagg cattgatata cactaaaaag aggcaagatt acctgcctct
     4681 ttttagttat taaatatacg tgttaattct tggtaatagt gttactgaga cgcattacga
     4741 agaaatttat cttgtttttc cttttttaaa aagaagtgaa gatatcctat aaagactcta
     4801 agtactatta cagtggctaa taacacttcg ataaacaaaa gacctttcca aatgtctgga
     4861 aacataagta caggcaaact gttctttaaa gcagttgctg agattactaa agggaatgtg
     4921 aaagctgaaa atacgggcga aaaaggttct tttagtaatt taggcaattg tataatgata
     4981 taaaaataaa atatttgagc taatagtaaa aatataatta cgataaaagc attcgcctta
     5041 ggaaaagcta taacatatgc tgcagcgact aaagaaaatg gtgcacaaat tgtcgatgtg
     5101 ttcggtttga ttgacgtctg caatggaaat gcttttaatc gcttgaaaac tataggtaag
     5161 acaatacaag tagctacaaa gccatatatt actgttaatt gacctatgaa aaaatatccg
     5221 ctaacgggtg ccgtcaatcc tgcgatagca ataccaataa aaagtacagt ccacgaagga
     5281 taaacatttt taagtgaaaa atcttttaaa tattttattg aaaaaataat catatgcgtc
     5341 ataattccca caaggcataa aatccaaata ggcgttatta agctattgat aaaagttatg
     5401 ttactaaaaa atgtatttaa ataagtagta cctaaaaagc cagacatgaa aaatgttgtg
     5461 aacactgatg aaactagagg actgtttaat tgttctttca cattattaaa atatttgatc
     5521 atagtacata aaaggtgaat ccaaatcaag aaagcaaaga ttccgcaaac agcgtttaaa
     5581 gtaagagata agtcttttaa tagattaccc aggcctaata atcctaagac tagtcctgac
     5641 gttactagag gtgctttttg aagtctcatg atttacaacc tttcttatgt gattttcttc
     5701 actaattata tcatgatagc tatggccaat taataagaag agtgtgtact attacgttat
     5761 tagagtatgt atattggatt gatagacaca taattgacat ttaaatctca aaattaatga
     5821 tatttgaggt atgctttgca acctaaatat tggaatatgt ggaaaagtaa ttatttaatt
     5881 taaatgatga ttgaagattt acaagggggt gtacaaaatg agaaatcaaa ttcaaaaact
     5941 attagacagt gatttgagca gtttacatat atcgaaacaa acaggagttc cacaaagcac
     6001 aatacacaga atgagaaaaa atgaaagatc attagacaat atgtcattga aaaacgctga
     6061 actactttat aaatttgcca atagtatatt tagcaatgaa aattaagaag aataattagt
     6121 gagttaaata attaaaaaac cacgtctcga tggacgtggt tatttttttt agatgtatat
     6181 aattataatt ataggatttc gggcccctga aaagtcccta aaattttgaa tagtatcaaa
     6241 atctatagga ataatataat aataaaaatc tctacgcatg caaatggtga gtgttaaaaa
     6301 tcttggtgta gtgttggtgt agttcaggtg cagtatagat gaaatttgag cacaaaatta
     6361 cttaattaaa aatgtataaa caagtgtcag acgcgtgtca attgtgtgtc aaaaaattct
     6421 ataacgaata agcatgcttg ttttaattac tgtattttcg gggcttatgc gggggacgtt
     6481 ttggtgacgc atcatactat attactgaaa ttcaaaacaa aaagagcccc gtaatcacgg
     6541 aactcttttg tttggtaatg cgtataaaaa tacctataaa cgtcctggga gggattcgaa
     6601 cccccgaccg atggcttaga aggccattgc tctatccagc tgagctacca ggacacgttt
     6661 aacaacacaa gaattattat atctaaatga acttaaatta gcaatacctt gtaaataaaa
     6721 aatgtttata tttttcacta ttatagagct atttatctaa aaaggttcaa taagacttaa
     6781 atacgaattc aggcaactta attgtgttaa atacagtttt gaatgcctaa ctgtatttct
     6841 tttctcttta aaatacagtt aagtacatta taagatgttg tgcggataaa caaactaatt
     6901 gtatcaaatt tattttaaaa taacaacaac aaaacgataa gcgaataaca tttcggtgat
     6961 ttaaaagcta cgcacgtttt tgttatcttc aaatttaaat tttaaggagt gttttcaatg
     7021 gaaggtttat ttaacgcaat taaagatacc gtaactgcag caattaataa tgatggcgca
     7081 aaattaggca caagcattgt gagcatcgtt gaaaatggcg taggtttatt aggtaaatta
     7141 ttcggattct aatttcaata tgttatgtaa gtaatcagta ttatttcaaa ggtgagggag
     7201 agatttaaat gactggacta gcagaagcaa tcgcaaatac tgtgcaagct gcacaacaac
     7261 atgatagtgt gaaattaggc acaagtatcg tagacatcgt tgctaacggt gtgggtttac
     7321 taggtaaatt atttggattc taatataata actaatattc tttaaaataa actgggtgag
     7381 catactttaa tgttatgcac tcagtttatt ttatttgcag aaatttgagc ctctgttaag
     7441 atttagatac atagacaata taggagatgg ggaaattggg atataaaaat attttgatag
     7501 actttgatga tacaattgtt gatttttatg atgcagaaga atgggcgttt cactatatgg
     7561 cgaatgtttt taatcataaa gcaacaaagg atgatttttt aacatttaaa aaaatcaatc
     7621 accaacattg ggaagctttt caacaaaata aattaacgaa gtctgaagta ttatcagaac
     7681 gatttgtgaa ttacttcaaa catcatcaaa tggaagttga tgggcatcgt gcagatgtgt
     7741 tatttagaaa tggattagca gaagctaaag ttaaatactt tgatcaaaca ttagaaacaa
     7801 ttgtcgaatt atcgaaaaga catgatttat atattgttac taatggtgta accgaaacgc
     7861 aaaagagaag gttaaatcag acgccgttgc ataaatatat taaaaagata tttatatctg
     7921 aagaaacagg atatcaaaaa cctaatccgg aattttttaa ttatgttttt aatgatattg
     7981 gtgaggatga aagacagcac tcgattatag ttggagattc tttaacatct gacattctag
     8041 gtggaatcaa tgcgggtata gctacttgct ggtttaattt tagaggattt gatcataatc
     8101 caggaattat acctgattat gaaattaatt catggaaaca actaaatgat attgtacgtt
     8161 aagacaaata atttgaaaat gatttactta tattagctta agaaagtaat tgtaaaaaag
     8221 acactacgaa aagatgcatt ttcatgctcg tagtgtcgaa tattattatc ttatggtttc
     8281 cacaataaga catcatgtcc ctctatattt ttagcttcca catctgtaaa tccatgatgt
     8341 aaaaagaaat ccttagaatc atttctgcca atggctttaa ttggcatatt gaaactcttt
     8401 gcaaaatcaa tcaattctga agcgtaccct ctgttttgat attttggtaa tacttctaac
     8461 ttccataata atatataatc ttcaaaatct gggaagtaga tttcttcgac atcacctttt
     8521 tttagtaatg ccattctagc tcctaattga tctccgacaa atatgccata aaatggtgaa
     8581 tctgaacttg catcaatcat ttgaccgttt aactcattga ccatgtataa gtctttgttg
     8641 ccaaacgctc taaagttttc gaataattcg tcagttttgt aattaatttc aagacgtttg
     8701 atttcactca tatttatcta ccccttattt gtttactact attattatac attaaaccac
     8761 ttgtgttttt cattgatttg aaaatgtaaa acaatgaata ttataaattt tccatgatta
     8821 aattgatttt caagccgtaa aattgtcaat caccggtata gactttatac tatagtttgt
     8881 aatcgcgtac ttaaggagaa aaaacatgga ctgtaaagta gttagtttaa atgaaaaaga
     8941 tcagtttata ccaaaaataa agagcagtga ccctgtaata acaggattat ttcaatatga
     9001 tgcagctcaa caaactagtt ttgaaaaaag gatgtctaaa gaaaataatg gaagagaagc
     9061 ggcattagcg aatgttattc gtgaatatat gagtgattta aagctttcaa gtgaacaaga
     9121 attaaacata caacatttag ctaatggttc aaaagttgtg attggtggac aacaagcagg
     9181 gcttttcggg ggaccattgt atacattcca taaaatattt tcaatcatta ctttatctaa
     9241 ggaattaacg gatacacata agcaacaagt agtaccagtt ttttggattg caggagaaga
     9301 tcatgatttc gatgaagtga atcatacatt tgtttataac gaaaatcatg ggtcgctgca
     9361 taaggttaaa tatcatacaa tggagatgcc agagacgact gtctctagat attatcctga
     9421 taaggctgag ttgaaacaaa ctttaaaaac gatgttcatt catatgaaag aaactgttca
     9481 tacacaaggt ctactggaga tttgtgacag aattattgac caatatgact cgtggactga
     9541 tatgtttaaa gcactactgc atgaaacatt taaagcatat ggcgttctat ttatagatgc
     9601 gcagtttgag ccgttaagaa aaatggaagc gcctatgttt aaaaagattt tgaaaaaaca
     9661 tcagttgctt gatgatgctt ttagagcaac acaacaacgt actcaaaatc aaggcttgaa
     9721 tgcgatgata caaacagata caaatgttca tttattctta catgatgaaa atatgcgtca
     9781 attagtttcg tatgatggta agcattttaa attaaataaa acagataaga catatataaa
     9841 ggaagaaatt ataaatattg cggaaaatca acctgaatta ttttctaata atgtagtgac
     9901 aagaccatta atggaagaat ggttatttaa cacggtggca tttgttggag gaccgagtga
     9961 aattaagtac tgggctgaac taaaagatgt atttgaacta tttgatgttg aaatgcctat
    10021 cgtgatgcca aggcttagaa ttacttattt aaatgaccgt atagaaaaat tactttcgaa
    10081 atacaatatt ccattagaaa aagtgttagt cgatggtgtt gaaggagaaa gaagtaagtt
    10141 tattagagaa caagcatcac atcaatttat tgaaaaggta gaaggtatga ttgaacaaca
    10201 gcgtcgtcta aacaaagact tattagatga agtggcgggg aatcaaaata atattaacct
    10261 tgtgaataaa aataatgaaa ttcatataca acagtatgat tatttgttaa aacgttatct
    10321 tttaaacatt gaaagagaaa acgacatcag tatgaagcaa tttagagaaa ttcaagaaac
    10381 actccatcca atgggaggat tacaagaaag aatatggaat ccacttcaaa ttttgaatga
    10441 ttttgggaca gatgtgttca agccctccac ctatccacca ctttcttaca cttttgatca
    10501 tattattata aaaccttaat ataccaaggg tttagcccga tttatcttaa tgataaatcg
    10561 ggcatttttt tgttttttaa aataaatttc acaaattttg tataaatagt ggtggatagt
    10621 ggggagatgt ggtaaattat atataaggtg aggtgataaa aaatgttcat gggagaatac
    10681 gatcatcaat tagatacaaa aggacgtatg attataccgt ccaagtttcg ttatgactta
    10741 aatgagcgtt ttattatcac aagaggcctt gataaatgtt tattcggtta cactctagac
    10801 gaatggcaac agattgaaga gaaaatgaaa accttaccta tgacaaaaaa agacgcacgt
    10861 aagtttatgc gtatgttctt ctctggtgct gttgaagtag aacttgataa gcaagggcgt
    10921 attaacatcc ctcaaaactt gaggaaatac gctaatttaa ctaaagaatg tacagtaatc
    10981 ggtgtttcaa atcgtattga gatttgggat agagaaactt ggaatgattt ctatgaagaa
    11041 tctgaagaaa gtttcgaaga tattgctgaa gatttaatag attttgattt ttaaaatgga
    11101 ggaattgaag tgtttcatca tatcagcgtt atgttaaacg aaaccattga ttatttaaat
    11161 gtaaaagaaa atggtgtgta cattgactgt acgctaggtg gagcgggaca tgccctttat
    11221 ttactaaatc aattaaatga cgacggaaga ttaatagcaa tcgatcaaga ccaaactgca
    11281 attgataatg ctaaagaggt attaaaggat catttgcata aggtgacttt tgttcatagc
    11341 aacttccgtg aattaactca aatattaaaa gacttaaaca ttgaaaaagt agatggaatt
    11401 tattacgact tgggtgtttc aagcccacaa ctcgacattc cagaacgagg attcagttat
    11461 caccatgacg caacattaga catgcgtatg gaccaaacac aagaactaac agcatatgaa
    11521 attgttaaca attggtcata tgaagcgtta gtgaagattt tttatcgcta tggcgaggag
    11581 aaattttcaa aacagatagc tcgaagaatc gaagcacatc gcgaacaaca accaataaca
    11641 acaacattag aattagttga cattataaaa gaaggtattc ctgcaaaagc aagaagaaaa
    11701 ggcggacatc ctgcaaaacg agtatttcaa gcactacgaa ttgcagtaaa cgatgaattg
    11761 tcagcttttg aagattcaat agaacaagcg attgaattag tgaaagtaga tggcaggatt
    11821 tcggtaatca ctttccattc tttagaagat cgtttatgta aacaggtgtt ccaagaatat
    11881 gaaaaaggtc cagaggtacc aagaggatta ccagttatac cagaagcata tacacctaag
    11941 ttaaagcgtg ttaatcgtaa accgattacc gctacagaag aagatttaga tgacaataac
    12001 agagcacgaa gcgcgaaatt acgtgtagct gaaatactta aataaggagc aatttataat
    12061 ggctgtagaa aaagtgtacc aaccatatga cgaacaagtt tataatagta taccgaagca
    12121 acaaccacaa actaagcccg aaaagaagac tgtttcgaga aaagtggttg tacaattaac
    12181 taaatttgaa aaagttttat acataacttt gattactgta attgctatgt taagtattta
    12241 tatgctatct ttaaaaatgg atgcgtatga tacgcgagga aagattgcag atttagatta
    12301 taaaatagat aaacaatcaa gtgaaaacag tgctttacaa tctgaaatca aaaagaattc
    12361 ttcttatgaa cgcatatacg aaaaggctaa gaaacagggg atgagccttg agaacgataa
    12421 tgtaaaggta gtgcgtagta atggcgaagc aaaaaattaa aattaaaaaa aataaaatag
    12481 gggcagtcct acttgttggt ttattcggac tgctcttttt tatattggtt ttaagaattt
    12541 catatatcat gattactgga cattctaatg gtcaagattt agtcatgaag gcaaatgaaa
    12601 agtatttagt taagaatgca caacaaccag aacgaggaaa gatatatgat cgtaatggta
    12661 aagtgctagc agaagatgta gaaagatata aacttgttgc agtaatagat aaaaaggcga
    12721 gtgccaattc taaaaaacct aggcatgtag ttgataaaaa agagactgca aagaaattat
    12781 ctacagtcat taatatgaag ccagaggaaa ttgaaaagag acttagtcaa aagaaagctt
    12841 tccaaattga atttggacgc aaaggaacaa atttaacgta tcaggacaaa ttgaaaatag
    12901 agaaaatgaa tttgcctggt atttctttat tgcctgaaac agaacgcttt tatccaaatg
    12961 gcaattttgc atcacactta attggtagag ctcagaaaaa tccggatact ggtgaactta
    13021 aaggtgcact tggagttgaa aagatttttg atagttattt aagtggatct aaaggatcat
    13081 tgagatatat tcatgatatt tggggatata tcgcaccaaa tactaaaaaa gagaagcagc
    13141 ctaaacgtgg tgatgatgtc catttaacaa tcgattcaaa tattcaagta tttgttgaag
    13201 aagctttaga tggcatggtt gaaagatacc agccgaaaga tttatttgcg gttgtcatgg
    13261 atgccaaaac tggagaaatt ttagcataca gtcagcgacc aacatttaat cctgaaactg
    13321 gtaaagactt tggtaaaaag tgggcaaatg acctttatca aaacacatac gagcctggat
    13381 caacatttaa atcatatggg ttagcagctg ctattcaaga aggtgctttt gatcctgata
    13441 agaaatataa atctggacat agagatatta tgggttcacg tatttcagac tggaatagag
    13501 tcggttgggg tgaaatccca atgtcactcg gatttactta ttcatctaat acattgatga
    13561 tgcatttaca agatttagtt ggtgcagaca aaatgaaatc ttggtatgaa cgatttggat
    13621 ttggaaaatc aactaaaggt atgtttgatg gagaagcacc tggtcaaatt ggatggagta
    13681 atgagttgca acaaaaaacg tcatcatttg gtcaatcgac aacagtaaca cctgttcaaa
    13741 tgttacaagc gcaatcagcg ttctttaatg atggtaatat gttaaaacca tggtttgtga
    13801 atagcgttga aaatcctgtt agtaaaagac aattttataa agggcaaaaa caaatcgcag
    13861 gcaaaccaat aacaaaagat actgctgaaa aagttgaaaa gcaattggat ttagttgtga
    13921 atagtaagaa gagtcacgct gcaaactatc gtattgatgg ttatgaggtc gaaggtaaga
    13981 ctggtacagc acaagtcgct gcacctaatg gtggtggata cgttaaaggt ccaaacccat
    14041 attttgtaag ttttatgggt gacgcgccga agaaaaatcc taaagttatt gtatacgctg
    14101 gtatgagctt ggcacaaaaa aatgaccaag aagcttatga attaggtgtt agtaaagcgt
    14161 ttaaaccaat aatggaaaat actttgaaat atttaaatgt aggtaaatca aaagatgaca
    14221 catctaatgc agagtatagt aaagtgccag atgttgaagg tcaagacaaa caaaaagcta
    14281 ttgataatgt gagtgcaaaa tcattagaac cagttactat tggttctggc acacaaataa
    14341 aagcacaatc tataaaagca gggaataaag tcttacctca tagtaaagta ctgttattaa
    14401 cagatggaga cttaactatg cctgacatgt caggatggac gaaagaagat gtcattgctt
    14461 ttgaaaacct aacaaatatt aaagtaaatt taaaaggtag cggttttgtg tcccaccaat
    14521 caattagtaa gggacaaaaa cttactgaaa aagataaaat agacgtagaa ttttcatcag
    14581 agaatgtaga cagcaattcg acgaataatt ctgattcaaa ttcagatgat aagaagaaat
    14641 ctgacagtaa aactgacaag gataagtcgg actaacaaga atggctacat taaattagga
    14701 tgtattgtca ctatttgttt ttactaaagt aatgctgtga tagaaaacaa catcttcatt
    14761 taatctttat aaataagtgt tgatagagaa ttttaaggta gacgaggtca gattaacttg
    14821 tttgttatag gcaaactttt tatactggcc tcttatgccg taaaaaagaa aaattataaa
    14881 ttttgattac aactttgttt atttaattga aaaattgata atatattagt ggttaaaaaa
    14941 gtctggatta agaagaggag atagttatga tttttgtata tgcgttatta gcgctagtga
    15001 ttacatttgt tttggtacct gttttaatac ctacattaaa aaggatgaaa tttggtcaaa
    15061 gtattcgaga agaaggtcca caaagccata tgaagaagac tggtacacca acgatgggtg
    15121 gactaacatt tctattaagt attgtgataa cgtctttggt ggctattata tttgtagatc
    15181 aagctaatcc aatcatactg ttattatttg tgacgattgg ttttgggtta attggtttta
    15241 tagatgatta tattattgtt gttaaaaaga ataaccaagg tttaacaagt aaacagaagt
    15301 ttttggcgca aattggtatt gcgattatat tctttgtttt aagtaatgtg tttcatttgg
    15361 tgaatttttc tacgagcata catattccat ttacgaatgt agcaatccca ctatcatttg
    15421 catatgttat tttcattgtt ttttggcaag taggtttttc taatgcagta aatttaacag
    15481 atggtttaga tggattagca actggactgt caattatcgg atttacaatg tatgccatca
    15541 tgagctttgt gttaggagaa acggcaattg gtattttctg tatcattatg ttgtttgcac
    15601 ttttaggatt tttaccatat aacattaacc ctgctaaagt gtttatggga gatacaggta
    15661 gcttagcttt aggtggtata tttgctacca tttcaatcat gcttaatcag gaattatcat
    15721 taatttttat aggtttagta ttcgtaattg aaacattatc tgttatgtta caagtcgcta
    15781 gctttaaatt gactggaaag cgtatattta aaatgagtcc gattcatcat cattttgaat
    15841 tgataggatg gagcgaatgg aaagtagtta cagtattttg ggctgttggt ctgatttcag
    15901 gtttaatcgg tttatggatt ggagtgcatt aagatgctta attatacagg gttagaaaat
    15961 aaaaatgtat tagttgtcgg tttggcaaaa agtggttatg aagcagctaa attattaagt
    16021 aaattaggtg cgaatgtaac tgtcaatgat ggaaaagact tatcacaaga tgctcatgca
    16081 aaagatttag aatctatggg catttctgtt gtaagtggaa gtcatccatt aacgttgctt
    16141 gataataatc caataattgt taaaaatcct ggaatacctt atacagtatc tattattgat
    16201 gaagcagtga aacgaggttt gaaaatttta acagaagttg agttaagtta tctaatctct
    16261 gaagcaccaa tcatagctgt aacgggtaca aatggtaaaa cgacagttac ttctctaatt
    16321 ggagatatgt ttaaaaaaag tcgcttaact ggaagattat ccggcaatat tggttatgtt
    16381 gcatctaaag tagcacaaga agtaaagcct acagattatt tagttacaga gttgtcgtca
    16441 ttccagttac ttggaatcga aaagtataaa ccacacattg ctataattac taacatttat
    16501 tcggcgcatc tagattacca tgaaaattta gaaaactatc aaaatgctaa aaagcaaata
    16561 tataaaaatc aaacggaaga ggattatttg atttgtaatt atcatcaaag acaagtgata
    16621 gagtcggaag aattaaaagc taagacattg tatttctcaa ctcaacaaga agttgatggt
    16681 atttatatta aagatggttt tatcgtttat aaaggtgttc gtattattaa cactgaagat
    16741 ctagtattgc ctggtgaaca taatttagaa aatatattag cagctgtgct tgcttgtatt
    16801 ttagctggtg tacctattaa agcaattatt gatagtttaa ctacattttc aggaatagag
    16861 catagattgc aatatgttgg tactaataga actaataaat attataatga ttccaaagca
    16921 acaaacacgc tagcaacaca gtttgcctta aattcattta atcaaccaat catttggtta
    16981 tgtggtggtt tggatcgagg gaatgaattt gacgaactca ttccttatat ggaaaatgtt
    17041 cgcgcgatgg ttgtattcgg acaaacgaaa gctaagtttg ctaaactagg taatagtcaa
    17101 gggaaatcgg tcattgaagc gaacaatgtc gaagacgctg ttgataaagt acaagatatt
    17161 atagaaccaa atgatgttgt attattgtca cctgcttgtg cgagttggga tcaatatagt
    17221 acttttgaag agcgtggaga gaaatttatt gaaagattcc gtgcccattt accatcttat
    17281 taaagggtgt gagtattgat ggatgataaa acgaagaacg atcaacaaga atcaaatgaa
    17341 gataaagatg aattagaatt atttacgagg aatacatcta agaaaagacg gcaaagaaaa
    17401 agatcaaagg ctacacattt ttctaatcaa aataaagatg atacatctca acaagctgat
    17461 tttgatgaag aaatttactt gataaataaa gacttcaaaa aagaagaaag caatgataaa
    17521 aataatgatt ctgcttctag tcatgcgaat gataataata tcgatgattc tacagactct
    17581 aatattgaaa atgaggatta tagatataat caagaaattg acgaccaaaa tgaatcgaat
    17641 gtaatttcag tcgacaacga acaacctcaa tcagctccta aagaacaaaa tagcgactcg
    17701 attgatgagg aaacagtaac gaaaaaagaa cgaaaaagta aagtaacaca attaaagcca
    17761 ttaacacttg aagaaaagcg gaagttaaga cgtaagcgac aaaagcgaat ccaatacagt
    17821 gttattacaa tattggtatt gttgattgct gttatattaa tttacatgtt ttcaccactt
    17881 agtaaaattg cgcatgtaaa tataaatgga aataatcacg ttagtacttc aaagataaac
    17941 aaagttttag gtgttaaaaa tgattcaagg atgtatacgt ttagtaaaaa aaatgctatt
    18001 aatgatctcg aagagaatcc attaatcaaa agtgttgaga tacacaagca attaccaaac
    18061 acattaaacg tagatatcac agaaaatgaa attattgctt tagtgaaata taaaggtaaa
    18121 tatttacctt tattagaaaa tggtaaattg cttaaaggtt caaatgatgt caaaattaat
    18181 gatgcacctg tcatggatgg tttcaaaggt acaaaagaag atgatatgat taaggcgtta
    18241 tctgaaatga cacctgaagt tagacgatat attgccgaag tgacatacgc cccaagtaaa
    18301 aacaaacaaa gcagaattga attgtttacg acagatggac ttcaagtaat cggtgatatt
    18361 tcgacgatat ctaagaaaat gaaatattat ccgcagatgt cacaatcatt atcaagggat
    18421 agttcgggta aactaaaaac acgaggctat attgatttat cagtcggtgc ttcatttatc
    18481 ccataccgtg gaaacacgtc tagtcaatca gaaagcgata aaaatgtgac taaatcatct
    18541 caagaggaaa atcaagcaaa agaagaatta caaagcgttt taaacaaaat taacaaacaa
    18601 tcaagtaaga ataattaaaa aattttgata ttgtctatgt ttatagttca caagccattc
    18661 aacgtattgt aaactaagga tagtgtattt ttttaatagt aatttgtcag gaggtgccta
    18721 tctatggaag aacattacta cgtaagtatt gatattggat catcaagcgt aaaaacaata
    18781 gtaggcgaga aatttcacaa tggtataaat gtgataggta caggacaaac ctacacgagc
    18841 ggtataaaaa atggtttaat tgatgatttt gatattgcgc gacaagcaat caaagacaca
    18901 attaaaaagg catcaatcgc ttcgggtgtt gatattaaag aagttttcct gaaattacct
    18961 atcattggaa cggaagttta tgatgaatca aatgaaatcg acttttatga ggatacagaa
    19021 atcaacggtt cacatatcga aaaagtatta gaaggtatta gagaaaaaaa tgatgtgcaa
    19081 gaaacagaag taattaatgt gttcccgatt cgttttatag tcgataaaga aaatgaggtt
    19141 tcagacccta aagaattaat tgccagacat tcattaaagg ttgaagcagg cgtaattgct
    19201 attcaaaaat cgattttaat taatatgatt aaatgcgtag aagcatgtgg tgttgatgta
    19261 ttagatgttt actctgatgc atataactat ggttcaatcc taacagctac tgaaaaagag
    19321 ttaggtgcat gtgtcattga tattggtgaa gacgttacgc aagttgcttt ttatgaacgc
    19381 ggtgaattag tagatgctga ttctatcgaa atggcagggc gtgatattac agacgatatt
    19441 gcacaaggat taaacacttc ttatgaaact gctgaaaaag ttaaacacca atatggtcat
    19501 gcattctatg attctgcttc agatcaagat atcttcactg ttgaacaggt tgatagtgat
    19561 gaaacagtac agtatactca aaaagatttg agtgacttta ttgaagcgcg tgtagaagaa
    19621 atattcttcg aagtatttga tgttttacaa gatttaggat taacaaaagt aaatggtggg
    19681 tttattgtaa ctggtggatc tgcaaactta cttggcgtaa aagaattatt atcagatatg
    19741 gtaagtgaaa aagttagaat tcacacgcca tcacaaatgg gaattagaaa acctgaattt
    19801 tcttcagcaa tttctacaat ttctagtagt atcgcttttg atgagttatt agattatgtt
    19861 acaattaatt atcatgataa tgaagaaact gaagaagatg ttattgatgt gaaagacaaa
    19921 gataacgaat ctaaattagg cggatttgat tggtttaaac gtaaaacaaa caaaaaagat
    19981 actcatgaaa atgaagtaga gtcaacagat gaagaaattt atcaatcaga agataatcat
    20041 caggaacata aacagaatca tgaacatgtt caagacaaag ataaagataa agaagaaagt
    20101 aaattcaaaa aactaatgaa atctctattt gaatgattat tggccaataa aactaggagg
    20161 aaatttaaat gttagaattt gaacaaggat ttaatcattt agcgacttta aaggtcattg
    20221 gtgtaggtgg tggcggtaac aacgccgtaa accgaatgat tgaccacgga atgaataatg
    20281 ttgaatttat cgctatcaac acagacggtc aagctttaaa cttatctaaa gctgaatcta
    20341 aaatccaaat cggtgaaaaa ttaacacgtg gtttaggagc aggagctaat cctgaaatcg
    20401 gtaaaaaagc tgcagaggaa tctcgtgaac aaattgaaga tgcaatccaa ggtgcagaca
    20461 tggtatttgt tacttctggt atgggtggcg gaactggtac tggtgcagca ccagtcgttg
    20521 ctaaaattgc aaaagaaatg ggcgcattaa ctgttggtgt tgtaactcgt ccatttagtt
    20581 ttgaaggacg taaacgtcaa actcaagctg ctgctggagt agaagctatg aaagctgcag
    20641 tagatacatt aatcgttata ccaaatgacc gtttattaga tatcgttgac aaatctacgc
    20701 caatgatgga agcatttaaa gaagctgaca acgtgttacg ccaaggtgta caaggtatct
    20761 cagacttaat cgctgtttct ggtgaagtaa acttagactt tgcagacgtt aagacaatta
    20821 tgtctaacca aggttctgca ttaatgggta ttggtgtttc ttctggtgaa aatagagcgg
    20881 tagaagctgc taaaaaagca atctcttctc cattacttga aacatctatc gttggtgcac
    20941 aaggtgtgct tatgaatatt actggtggcg agtcattgtc attatttgaa gcacaagagg
    21001 ctgctgatat tgtccaagat gctgcagatg aagacgttaa tatgattttc ggtacagtta
    21061 ttaatcctga attacaagat gagattgttg taacagttat tgcaactggt tttgatgaca
    21121 aaccaacatc acatggtcgt aaatctggta gcactggatt cggaacaagc gtaaatactt
    21181 ctagcaatgc aacttctaaa gatgaatcat tcacttcaaa ttcatcaaat gcacaagcaa
    21241 ctgatagtgt aagtgaaaga acacatacaa ctaaagaaga tgatattcct agcttcatta
    21301 gaaatagaga agaaagacgt tcaagaagaa caagacgtta atcggttaat atatatacac
    21361 aaataattca acacaaatca tcagataaca tatctgatga tttttttact aatttttaga
    21421 acatgtagaa ggacatttaa gtttttcaaa gttattaaaa gtgtttaagt atcgtgtgaa
    21481 aattaagtca aaaattattt gcgcaacatt ttaacttt
//
LOCUS       DALAGD010000051        21399 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.051, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000051 DALAGD010000000
VERSION     DALAGD010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 21399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21399
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.051"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            280..957
                     /gene="recU"
                     /locus_tag="O2701_001514"
     CDS             280..957
                     /gene="recU"
                     /locus_tag="O2701_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HCZ4293409.1"
                     /translation="MLCIINNVYYQTCVVKFMNYPNGKPYRKNSAIDGGKKTAAFSNI
                     EYGGRGMSLEKDIEHSNTFYLKSDIAVIHKKPTPVQIVNVNYPKRSKAVINEAYFRTP
                     STTDYNGVYQGYYIDFEAKETKNKTSFPLNNIHDHQVEHMKNAYQQKGIVFLMIRFKT
                     LDEVYLLPYSKFEVFWKRYKDNIKKSITVDEIRKNGYHIPYQYQPRLDYLKAVDKLIL
                     DESEDRV"
     gene            954..3137
                     /locus_tag="O2701_001515"
     CDS             954..3137
                     /locus_tag="O2701_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCZ4293410.1"
                     /translation="MTENKGSSQPKKNGNNGGKSNSKKNRNVKRTIIKIIGFMIIAFF
                     VVLLLGILLFAYYAWKAPAFTEAKLQDPIPAKIYDKNGELVKTLDNGQRHEHVNLKDV
                     PKSMKDAVLATEDNRFYEHGALDYKRLFGAIGKNLTGGFGSEGASTLTQQVVKDAFLS
                     QHKSIGRKAQEAYLSYRLEQEYSKDDIFQVYLNKIYYSDGVTGIKAAAKYYFNKDLKD
                     LNLAEEAYLAGLPQVPNNYNIYDHPKAAEDRKNTVLYLMHYHKRITDKQWEDAKKIDL
                     KANLVNRTPEERQNIDTNQDSEYNSYVNFVKSELMNNKAFKDENLGNVLQSGIKIYTN
                     MDKDVQKTLQNDVDNGSFYKNKDQQVGATILDSKTGGLVAISGGRDFKDVVNRNQATD
                     PHPTGSSLKPFLAYGPAIENMKWATNHAIQDESSYQVDGSTFRNYDTKSHGTVSIYDA
                     LRQSFNIPALKAWQSVKQNAGNDAPKKFAAKLGLNYEGDIGPSEVLGGSASEFSPTQL
                     ASAFAAIANGGTYNNAHSIQKVVTRDGETIEYDHTSHKAMSDYTAYMLAEMLKGTFKP
                     YGSAYGHGVSGVNMGAKTGTGTYGAETYSQYNLPDNAAKDVWINGFTPQYTMSVWMGF
                     SKVKQYGENSFVGHSQQEYPQFLYENVMSKISSRDGEDFKRPSSVSGSIPSINVSGSQ
                     DNNTTNRSTHGGSDTSANSSGTAQSNNNTRSQQSRNSGGLTGIFN"
     gene            complement(3703..4044)
                     /locus_tag="O2701_001516"
     CDS             complement(3703..4044)
                     /locus_tag="O2701_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293411.1"
                     /translation="MTNITKEVFDNLEQEIDLFAKNKTLGSSEAKPYLDEYHSKIIDY
                     FKQVNDITGNIDFDKLNQYPVVPMNFKERYDYMIERKYHFMGYRQMKTFKTELIKMNA
                     SYQTRLKNKQV"
     gene            complement(4049..4708)
                     /gene="nth"
                     /locus_tag="O2701_001517"
     CDS             complement(4049..4708)
                     /gene="nth"
                     /locus_tag="O2701_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCZ4293412.1"
                     /translation="MVSKKKALEMIDVIANMFPDAECELKHDNPFELTIAVLLSAQCT
                     DVLVNRVTTELFKKYKTPEDYLAVSDEELMNDIRSIGLYRNKAKNIKKLCQSLIDQFN
                     GEIPQTHKELESLAGVGRKTANVVMSVAFDEPSLAVDTHVERVSKRLGINRWKDNVRQ
                     VEDRLCSVIPRDRWNRSHHQLIFFGRYHCLARKPKCDICPLLEDCREGQKRYKASLKE
                     A"
     gene            complement(4698..5384)
                     /locus_tag="O2701_001518"
     CDS             complement(4698..5384)
                     /locus_tag="O2701_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HCZ4293413.1"
                     /translation="MDKYQLKARPVVIRRELLDHYSDLGLDEQDLVILLKLIYASETS
                     NKQPSIELLQKGSTMQPRDITMVIQNLIQRELLELQVQKDEEGRFTEYMNLDPFFEKL
                     SHILKQQSMETKELNSKEKFKQLFRVLEDTFARPLSPYEIETLNQWIDVDKHDTAIIQ
                     AALDEANSLNKLSFKYIDRILLNWKKNNVKTIDDSRKIREKFNKPKMTHTVKTVPKFD
                     WLNGENLDGK"
     gene            complement(5712..7004)
                     /gene="asnS"
                     /locus_tag="O2701_001519"
     CDS             complement(5712..7004)
                     /gene="asnS"
                     /locus_tag="O2701_001519"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCZ4293414.1"
                     /translation="MKTTIKQAKDHLNQDVTIGAWLTNKRSSGKIAFLQLRDGTGFMQ
                     GVVVKSEVDEEVFKLAKEITQESSLYVTGTITEDNRSDLGYEMQVKSIEVISEAHDYP
                     ITPKNHGTEFLMDHRHLWLRSKKQHAVMKIRNEVIRATYEFFNKDGFTKVDPPILTAS
                     APEGTSELFHTKYFDQDAFLSQSGQLYLEAAAMAHGKVFSFGPTFRAEKSKTRRHLIE
                     FWMIEGEMAFTNHAESLEIQEQYVTHVVKSVLENCKLELKILERDTSKLEKVATPFPR
                     ISYDDAIEFLKAEGFDDIEWGEDFGAPHETAIANHYDLPVFITNYPTKIKPFYMQPNP
                     ENEETVLCADLIAPEGYGEIIGGSERVDDLELLEQRVKEHGLDEEAYSYYLDLRRYGS
                     VPHCGFGLGLERTVAWISGVEHVRETAPFPRLLNRLYP"
     gene            complement(7326..10019)
                     /locus_tag="O2701_001520"
     CDS             complement(7326..10019)
                     /locus_tag="O2701_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase DinG"
                     /protein_id="HCZ4293415.1"
                     /translation="MGMATYAVVDLETTGNQLDFDDIIQIGITFVRNNQIIDTYHSMI
                     RTNLEIPPFIQALTSIEENMLQQAPYFNQVAQEIYDKIKDCIFVAHNVDFDLNFIKKA
                     FKDCNIQYRPKKVIDTLEIFKIAFPTDKSYQLSELAEAHGITLANAHRADEDAATTAK
                     LMILAFEKFEKLPLDTLKQLYYLSKQLKYDLYDIFFEMVRQYDAKPLDKSYEKFEQII
                     YRKQVDFKKPTTNYNGSLKSLYSKAVDQLGLTYRPQQLYLAETILDQLMHSEKAMIEA
                     SLGSGKSLAYLLAALMYNIETGKHVMISTNTKLLQSQLLEKDIPAMNEALNFKINALL
                     IKSKSDYISLGLISQILKDDTSNYEVNILKMQLLIWITETPSGDIQELNLKGGQKMYF
                     DQKIETYVPARHDVHYYNFIKRNAQNIQIGITNHAHLIHSDVENSIYQLFDDCIVDEA
                     HRLPDYALNQVTNELSYADIKYQLGLIGKNENEKLLKAIDQLEKQRILEKLDIAPIDI
                     FGLKASMNEIHELNEQLFSTIFTIINDSDVYDDDIHRFHNVFTFETKDILKDLHAIID
                     KLNKTLEIFNGISHKTVKSLRKQLLYLKDKFKNIEQSLKAGHTSFISIKNLSQKSTIR
                     LYVKDYAVKDVLTKQVLEKFKSLIFISGTLKFNHSFEAFKQLFNKDVHFNTFEVNTSL
                     QSAKNTSVFIPSDVASYQYKNIDEYVASIVSYIIEYTTITSSKCLVLFTSYKMMHMVQ
                     DMLNELPEFEDYVVLTQQQNQNYKIVQQFNNFDKAILLGTSTFFEGFDFQANGIKCVM
                     IAKLPFMNKHNAKYWLMDSEFTSTFKEYVLPDAVTRFRQGLGRLIRNENDRGIIVSFD
                     DRLINSNYKNFFEQTLENYRQKKGDIQQFGKLLRQIQKKKK"
     gene            complement(10043..11014)
                     /locus_tag="O2701_001521"
     CDS             complement(10043..11014)
                     /locus_tag="O2701_001521"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HCZ4293416.1"
                     /translation="MSKYSQDVLQLLYKNKPNYISGQSIAESLNISRTAVKKVIDQLK
                     LEGCKIDSVNHKGHLLQQLPDIWYQGIIDQYTKSSALFDFSEVYDSIDSTQLAAKKSL
                     VGNQSSFFILSDEQTKGRGRFNRHWSSSKGQGLWMSVVLRPNVAFSMISKFNLFIALG
                     IRDAIQHFSQDEVKVKWPNDIYIDNGKVCGFLTEMVANNDGIEAIICGIGINLTQQLE
                     NFDESIRHRATSIQLHDKNKLDRYQFLEILLQEIEKRYNQFLTLPFSEIREEYTAASN
                     IWNRTLLFTENDKQFKGQAIDLDYDGYLIVRDEAGESHRLISADIDF"
     gene            complement(11001..12203)
                     /locus_tag="O2701_001522"
     CDS             complement(11001..12203)
                     /locus_tag="O2701_001522"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HCZ4293417.1"
                     /translation="MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDI
                     TTSATPDEIESIFSHTIPVGKEHGTINVVFNDENYEVTTFRAEEDYVDHRRPSGVTFV
                     RDLYEDLQRRDFTMNAIAMDTAYKLYDYFDGQQDINNRIIRTVGIAEERFQEDALRMI
                     RCLRFQSQLSFDIATETFEAMRIQMADIKFLSIERIVIELTKLMRGINVEKSFNHLKS
                     LKAFNYMPYFEHLDMNQINVTEAIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQ
                     YIQIMNALPSIITKEQLKMFVYDYDTHLIKNVMVAADVLKANDIQGHEPLIVNLQTID
                     ETLHRLPMHNRKDMMVNGGVLMAHLNAKSGPWLKDVLRQIEIAIVTGKVSNEETEILK
                     WVDNHVKI"
     gene            complement(12208..13350)
                     /gene="bshA"
                     /locus_tag="O2701_001523"
     CDS             complement(12208..13350)
                     /gene="bshA"
                     /locus_tag="O2701_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HCZ4293418.1"
                     /translation="MKIGITCYPSMGGSGIIATELGIKLAERGHEVHFITSNIPFRIR
                     KPLPNMIFHQVEVNQYAVFQYPPYDITLSTKIAEVIKEYDLDLLHMHYAVPHAICGIL
                     AREMSGKDIKIMTTLHGTDITVLGYDHSLQGAIKFGIEKSDIVTSVSKSLAQETHEII
                     ETNKEIIPIYNFVRENEFPTKHNTALKSQFGIAPDEKVLIHVSNFRQVKRIDTIIETF
                     AKVREKIPSKLILLGDGPELVPMRQLTKELNVEEDVLFLGKQDCVSEFYQLSDLVLLL
                     SEKESFGLTLLEAMKTGVVPIGSNAGGIKEVIKHGETGFVVDVGDCDSASDYAIRLLE
                     DKVLYNKLQKNMLADIAERFGSELITDQYEYYYQKMLNEHNKSKGE"
     gene            complement(13591..13908)
                     /locus_tag="O2701_001524"
     CDS             complement(13591..13908)
                     /locus_tag="O2701_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCZ4293419.1"
                     /translation="MKSMVEMQREVDEYIGQFKTGYFSPLANLARLTEEVGELAREIN
                     HTYGEKKKKDSEEANTIKAELGDNLFVLLCLANSMGIDMTESFNETMEKFNTRDKNRF
                     ERK"
     gene            complement(14245..14925)
                     /locus_tag="O2701_001525"
     CDS             complement(14245..14925)
                     /locus_tag="O2701_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HCZ4293420.1"
                     /translation="MIIYFVILMVLPLWAQHKVKSNYEKYSQVRSTSGKTGREVALEI
                     LHANGIYDVEVVKGEGFLTDHYDPKKKVVSLSPANYDRPSVAGTAIAAHEVGHAIQDH
                     QGYWFLRFRAALVPVANLGSSLSYMIIMLGIILTAIGSAFGSTALWIGAGLMSLAVLF
                     SIVTLPVEFDASSRAMKQITALNIVNEKEYKHARKVLSAAAMTYVAATAVAVAELVRI
                     ILIARSSD"
     gene            complement(14997..15584)
                     /locus_tag="O2701_001526"
     CDS             complement(14997..15584)
                     /locus_tag="O2701_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1405 domain-containing protein"
                     /protein_id="HCZ4293421.1"
                     /translation="MTIKAFWQYTLYQRSWLMMLLICNILGMIYGYIWYGEQLSHTPW
                     HFKIFVPDSPTAILYLVISISLILIQKQNSIIDALAFVTLFKYGIWAVIMNILFIIEQ
                     GDITVNGLVLMFSHSIMAVQAIYFYPRFKRSMIGISVAMIWVFLNDYIDYFHLQFPYY
                     DFITTHVWQIGVLSCCLSVFGLLLYIELNKLLKCK"
     gene            complement(15574..16149)
                     /locus_tag="O2701_001527"
     CDS             complement(15574..16149)
                     /locus_tag="O2701_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HCZ4293422.1"
                     /translation="MSETFNQIKESFIEYLLFQYRFKSRIAVWVLNYIKVNEAKLANI
                     HFVDTKINHHETLEIAEVGSHASAIQFTKRNIKLMNTNEIFDYIANHNCAFDIQIHFA
                     NVSKREQRLDDLIVAQLTESPSYQTYLHDLNSMAIDRHKHALLIDYLLHNIDLSLQMN
                     EKQRFYQLTQILNTLKLVNKHNQFEDLADDN"
     gene            complement(16163..17407)
                     /locus_tag="O2701_001528"
     CDS             complement(16163..17407)
                     /locus_tag="O2701_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293423.1"
                     /translation="MEDIYKLIDDINLQKLENLDSRVNEAITTDNDDALFILGETLYN
                     FGLMPQGLEVFRVLYHKYPDESELLIYFIEGLMSENQTDEALEYLSYVEPSPEKLMLE
                     ADLYQQINMMEVAIDKLQEALELEPNDPIIHFALAEMLYYDGQYLRATSEYETVLETG
                     EYQVNGVNLFSRMADCSLQSGNYSDAIRLYDEINEDEMTSEDYLKKAISYDKNDITQE
                     AIKIMTTLLSKDPDYIQGYLYLQSLYENEKNYPDAIETGKEGLRLSQFYKELMYTTGC
                     LEIEHGDANEGVLLLKQALEVDNAYQEPLLILSDLYRNEEDYEAIIELLTYVDEEDLD
                     PTFMWHLAFAYGQEERDKEAQHFFELAYPTMKTNIDFMSDYYFYLIEIGQKEQAITIL
                     NQLLELEPSNENWHDELSRLQS"
     gene            complement(17414..18712)
                     /gene="aroA"
                     /locus_tag="O2701_001529"
     CDS             complement(17414..18712)
                     /gene="aroA"
                     /locus_tag="O2701_001529"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCZ4293424.1"
                     /translation="MVNEQIIDISGPLKGEIEVPGDKSMTHRAIMLASLAEGVSTIYK
                     PLLGEDCRRTMDIFRLLGVEIKEDDEKLVVTSPGYQSFNTPHQVLYTGNSGTTTRLLA
                     GLLSGLGIESVLSGDVSIGKRPMDRVLRPLKLMDANIEGIEDNYTPLIIKPSVIKGIN
                     YQMEVASAQVKSAILFASLFSKEPTIIKELDVSRNHTETMFKHFNIPIEAEGLSINTT
                     PEAIRYIKPADFHVPGDISSAAFFIVAALITPGSDVTIHNVGINPTRSGIIDIVEKMG
                     GNIQLFNQTTGAEPTASIRIQYTPMLQPITIEGELVPKAIDELPVIALLCTQAVGTST
                     IKDAEELKVKETNRIDTTADMLNLLGFELQPTNDGLIIHPSEFKTNATVDSLTDHRIG
                     MMLAVASLLSSEPVKIKQFDAVNVSFPGFLPKLKLLENEG"
     gene            complement(18722..19786)
                     /locus_tag="O2701_001530"
     CDS             complement(18722..19786)
                     /locus_tag="O2701_001530"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCZ4293425.1"
                     /translation="MKLQTTYPSNNYPIYVEHGAIDHISTYIDQFDQSFILIDEHVNQ
                     YFADKFDDILSYENVHKVIIPAGEKTKTFEQYQETLEYILSHHVTRNTAIIAVGGGAT
                     GDFAGFIAATLLRGVHFIQVPTTILAHDSSVGGKVGINSKQGKNLIGAFYRPTAVIYD
                     LDFLKTLPFEQILSGYAEVYKHALLNGESATQDIEQHFKDREILQSLNGMDKYIAKGI
                     ETKLDIVIADEKEQGVRKFLNLGHTFGHAVEYYHKIPHGHAVMVGIIYQFIVANALFD
                     SKHDINHYIQYLIQLGYPLDMITDLDFETLYQYMLSDKKNDKQGVQMVLIRQFGDIVV
                     QHVDQLTLQHACEQLKTYFK"
     gene            complement(19812..20978)
                     /gene="aroC"
                     /locus_tag="O2701_001531"
     CDS             complement(19812..20978)
                     /gene="aroC"
                     /locus_tag="O2701_001531"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCZ4293426.1"
                     /translation="MRYLTSGESHGPQLTVIVEGVPANIEIKVEDINKEMFKRQGGYG
                     RGRRMQIEKDTVEIVSGVRNGYTLGSPITMVVTNDDFTHWRKIMGAAPISEEERENMK
                     RTITKPRPGHADLVGGMKYNHRDLRNVLERSSARETAARVAVGALCKVLLQQLDIDIY
                     SRVVEIGGIKDKDFYDSETFKANLDRNDVRVIDDSIAQAMRDKIDEAKNEGDSIGGVV
                     QVVVENMPVGVGSYVHYDRKLDGKIAQGVVSINAFKGVSFGEGFKAAEKPGSEIQDEI
                     LYNSEIGYYRGSNHLGGLEGGMSNGMPIIVNGVMKPIPTLYKPLNSVDINTKEDFKAT
                     IERSDSCAVPAASIVCEHVVAFEIAKALLEEFQSNHIEQLKQQIIERRQLNIEF"
BASE COUNT         6947 a         3834 c         2979 g         7639 t
ORIGIN      
        1 atacacctct gctgattaac aacacatact tgtacttgcc tcaaaaataa aaattactaa
       61 tcatgatttg acttttataa caaaattcaa aaatattgta atgagtattc atttttataa
      121 gcaaaacttt tcaattaatt aaaattgtgt gtaaaattcc aacattaacg gtaaatgtat
      181 tataatatta ttgtgttaat gctgaaatca cgttaaaata agctttggtg tattgtgtct
      241 ttcgcattta tactaattta agtttggtgt ttcactataa tgttgtgtat aataaacaac
      301 gtttactacc aaacttgtgt ggtgaaattt atgaattatc caaatggtaa accatatcgt
      361 aaaaatagtg ctatagacgg agggaaaaag accgctgcct ttagtaatat tgagtatggt
      421 ggacgtggta tgtcacttga aaaagatatc gaacattcaa atacgtttta tcttaaaagc
      481 gacattgcag ttattcacaa aaagcctacg ccagtacaaa tagttaatgt caactatcct
      541 aagcggagta aagctgtgat taacgaagct tattttcgta caccttcaac aactgattac
      601 aacggcgttt atcaaggtta ttatattgat tttgaagcaa aggaaactaa aaacaagacg
      661 tcctttcctt taaataatat tcatgaccat caagtcgaac atatgaaaaa tgcatatcaa
      721 caaaaaggta ttgtgttttt aatgattcgt tttaaaacgc tagatgaagt ttatctttta
      781 ccctattcaa aattcgaagt attttggaag agatataaag ataatattaa aaagtctata
      841 acagttgatg aaatacgaaa aaatggttac catattcctt atcagtatca accaagatta
      901 gactatctaa aagcagttga taagttgata ttagatgaaa gtgaggaccg cgtatgacgg
      961 aaaacaaagg atcttctcag cctaagaaaa acggtaataa tggtgggaaa tccaactcaa
     1021 aaaagaatag aaatgtgaag agaacgatta ttaagattat tggcttcatg attattgcat
     1081 ttttcgttgt tcttttacta ggtatcttat tgtttgctta ttatgcttgg aaagcacctg
     1141 cttttaccga agctaaatta caagatccga ttcctgcaaa gatatatgac aagaacggag
     1201 aacttgttaa aacattagat aatggccaaa gacatgagca tgtaaattta aaagacgtgc
     1261 cgaaatcaat gaaagacgca gtacttgcaa ctgaagacaa tcgtttctac gaacatggcg
     1321 cacttgatta taaacgttta ttcggtgcaa ttggtaagaa cttgactggt ggatttggtt
     1381 ctgaaggtgc ctcaacatta acacaacaag ttgttaaaga tgcattttta tcacaacata
     1441 aatctattgg acgtaaagct caagaagcat acttatcata tcgtttagaa caagagtata
     1501 gtaaagatga tatcttccaa gtatatctaa acaaaattta ctattctgat ggcgtaacag
     1561 gtattaaagc tgctgctaag tattacttta ataaagattt aaaagattta aacttagcgg
     1621 aagaagctta tttagccggt ttacctcagg ttccaaacaa ctataatatt tatgatcatc
     1681 caaaagctgc tgaagatcgt aaaaacactg ttttatactt aatgcattat cataaacgca
     1741 ttacagataa acagtgggaa gatgctaaga aaatcgattt aaaagcgaac ttagtaaatc
     1801 gtactcctga agaacgtcaa aacattgata caaatcaaga ttctgagtat aattcatacg
     1861 ttaactttgt aaaatctgaa ttaatgaata ataaagcatt caaagatgaa aatttaggta
     1921 atgtattaca aagtggtatt aaaatttata caaacatgga taaagatgtt caaaaaacat
     1981 tacaaaatga tgttgataat ggtagcttct acaagaataa agaccaacaa gttggtgcaa
     2041 caattcttga tagtaaaact ggtggtttag ttgctatatc tggtggacgt gatttcaaag
     2101 acgtcgttaa cagaaaccaa gcaacagatc ctcaccctac tggttcatct ttaaaacctt
     2161 tcttagcgta tggacctgcc attgaaaata tgaaatgggc aacaaaccat gcgattcaag
     2221 atgaatcttc atatcaagtt gatggttcta catttagaaa ctatgatacg aagagtcacg
     2281 gtactgtatc tatttatgat gctttacgac aaagtttcaa tatcccagct ttaaaagctt
     2341 ggcaatcagt taagcaaaat gctggtaatg atgcacctaa gaaattcgct gccaaacttg
     2401 gcttaaacta cgaaggcgat attggtccat ctgaagtact tggtggttct gcttcagaat
     2461 tctcaccaac acaattagca tcagcatttg ctgcaatcgc taacggtggt acttataaca
     2521 acgcgcattc aattcaaaaa gtagttactc gtgatggtga aacaatcgaa tacgatcata
     2581 ctagccataa agcgatgagt gattacactg catacatgtt agctgagatg ctaaaaggta
     2641 catttaaacc atatggttct gcatatggcc atggtgtatc tggagtaaat atgggtgcta
     2701 agacaggtac tggtacttac ggtgctgaaa cttattcaca atataattta cctgataatg
     2761 cagcgaaaga cgtgtggatt aacggcttta cacctcaata cactatgtca gtgtggatgg
     2821 gcttcagtaa agttaaacaa tatggtgaaa actcatttgt gggacatagc caacaagaat
     2881 atccacagtt cttatatgaa aatgtgatgt caaaaatttc atctagagat ggcgaagact
     2941 ttaaacgtcc tagctcagta agtggtagta tcccatcaat caatgtttct ggtagtcaag
     3001 ataacaacac tacaaatcgt agtacacacg gtggtagtga cacatcagca aacagcagtg
     3061 gtactgcaca atcaaataac aatactagat ctcaacaatc tagaaacagc ggtggattaa
     3121 caggtatatt caactaatcc actcaacata aaatcctcag ttataccata tttatggtgt
     3181 agccgaggat ttttttaggt tcttcatctt ttatggtggg aaggtaaaac ttcctgcttt
     3241 ttttataagt attcatacgc tattgctata ttagtagaaa ccgtaaaatc agtcattcac
     3301 atacattttg aaacatctaa atagtttttc atattcacat ctttagctat gtaaaattat
     3361 tgaatttgaa taagtggttg ttcccatttt taaaattatg attatatttt ctcatagtca
     3421 tcttagacgc tgttttaaat tgatatattt attaattccc tgtgtacgtt aatcagtgaa
     3481 atatcaacat gtttgcaaca tattttcaca atcatagata taagaaatcc acctacgatt
     3541 cgaaatagat atcttaaagc atatctcaag atatccatta tgatgtagat ggacagtttt
     3601 tatattaaga cgttgtaatg acacgacacc taacataatt ataaatatgt gtgcatgaat
     3661 tagttttata tattttcaac attaggaaat gatcaatctt gtctatacct gcttattctt
     3721 taaccttgtt tgataactag cattcatttt aatcaattca gttttaaagg tcttcatttg
     3781 tcgatagccc ataaaatgat atttacgttc aatcatataa tcatatcttt ctttaaaatt
     3841 cattggcaca acaggatatt gatttaattt atcaaaatca atattgccag ttatgtcatt
     3901 aacctgctta aaataatcaa taattttact atggtattca tccaagtagg gctttgcttc
     3961 actagaacct aatgtcttat ttttggcaaa taaatcaatc tcttgttcta aattatcaaa
     4021 tacctctttt gtaatatttg tcatgttatc acgcttcttt caaactagct ttataacgtt
     4081 tttgtccttc tctacaatct tctaatagtg gacaaatatc gcatttaggt tttcgggcta
     4141 aacagtggta tcttccaaag aaaatgagtt gatgatggct cctattccat ctatctctag
     4201 gtatgacaga acataaacgg tcttctacct gtctcacatt atctttccaa cgattaatac
     4261 ctaagcgttt agaaacacgt tctacatgcg tatcaacagc taatgaaggt tcatcaaatg
     4321 ctacactcat gactacatta gcagttttac gtcctacacc tgctaaactt tctaattcct
     4381 tatgtgtttg tggtatttct ccattaaatt gatcaatcaa agattgacaa agtttcttaa
     4441 tattcttagc tttgttacga tacagaccga tagaacgaat atcattcata agttcttcat
     4501 cactgactgc caaataatct tcaggcgttt tgtatttttt aaacagctca gttgttactc
     4561 tatttactag aacgtctgta cattgcgctg acaataatac agcaatagtt aattcgaacg
     4621 gattatcatg ttttaattca cattctgcat ccggaaacat atttgctata acatcaatca
     4681 tttctaatgc ttttttctta cttaccatca aggttctccc cgtttaacca atcaaattta
     4741 ggtaccgttt taactgtgtg cgtcattttc ggtttattga atttttctct tatttttcta
     4801 gaatcgtcaa ttgttttgac attgtttttc ttccaattaa gtaaaatacg atctatatat
     4861 ttaaagctaa gtttattcaa actattcgcc tcgtctaatg ccgcttgtat aattgcagta
     4921 tcgtgtttat caacatcaat ccattgattt aacgtttcta tttcatatgg agataacggc
     4981 cttgcaaatg tatcctctaa aactctaaat aattgtttaa atttttcttt actatttagc
     5041 tctttcgttt ccatactttg ttgcttcaat atatgactta atttttcgaa aaaaggatct
     5101 agattcatat attcggtaaa tctaccttct tcatcttttt gaacttgtaa ttctagcaat
     5161 tcacgttgta tcaaattttg aataaccatt gtaatatcac gtggttgcat agttgagccc
     5221 ttctgaagca attcaattga aggctgttta tttgatgttt cggaagcata aatcaattta
     5281 agcaaaatga ctaaatcttg ctcatctaaa cctaagtcac tgtaatggtc taataattct
     5341 cttcgtatca ctacaggtct tgcttttaat tgatatttat ccatgttcgt gccccctttt
     5401 aaatttaacc attacttaat agtataaatc attttcttta attttcgact aaaatgctta
     5461 atatatttaa ctagtagcaa acaaatttta aataacagta ttttttaaca ctagatttta
     5521 acaatgaact ttatggataa tcagatgaac tatttatttt ccaaatatag ttactaaaca
     5581 aaagtttgtt catgattaat ataagccgtt atcaatcttc attttccaag aataaatcgc
     5641 ccagctatta atataggctg gacgattctt tttattatca taatatatat tttaagtaac
     5701 gaccctaaaa cttatggata taaacggttt aataatcttg ggaatggcgc tgtttcacga
     5761 acgtgttcaa caccagaaat ccatgctact gtacgctcta aacctaaacc aaatccacag
     5821 tgtggcacac taccataacg acgtaagtct aagtagtaac tatatgcttc ttcgtctaat
     5881 ccatgttctt taacgcgttg ttctaacaat tctaagtcat ccacacgttc agatccacca
     5941 ataatttcac cgtatccttc aggtgcaatt aagtctgcac ataatacagt ttcttcattt
     6001 tcaggatttg gttgcatata gaaaggctta attttagttg gataattagt aataaacacc
     6061 ggtaaatcat aatgattagc aatggctgtt tcatgtggcg caccaaaatc ttcgccccat
     6121 tcaatatcat caaagccttc tgcttttaag aattcaattg catcatcata tgaaattcta
     6181 gggaatggtg tcgcaacttt ttcaagtttt gatgtatcac gctctaaaat tttcaactct
     6241 agtttacaat tttctaaaac tgattttaca acatgtgtta catattgttc ttgaatttct
     6301 aaactttcag catgatttgt gaaagccatt tctccttcaa tcatccagaa ctcgatcaag
     6361 tgtctacgtg tttttgattt ttcagctctg aaagttggac caaatgaaaa tacttttccg
     6421 tgtgccattg ctgcagcttc taagtataac tgaccacttt gagataaaaa cgcatcttga
     6481 tcaaagtatt tagtatggaa taattcactt gtaccttctg gcgcacttgc tgtcaaaatt
     6541 ggtggatcaa cctttgtaaa tccatctttg ttgaaaaatt catacgttgc acgaataact
     6601 tcatttctaa ttttcattac agcatgttgt tttttagaac gtaaccataa atgacggtga
     6661 tccattaaga attctgtacc atgattttta ggtgtaatcg gatagtcatg cgcttctgaa
     6721 ataacttcaa ttgatttcac ttgcatttcg tatcctaagt cagaacgatt atcttctgta
     6781 attgtgcctg taacgtatag agatgattct tgagtaattt ctttcgcaag tttgaatacc
     6841 tcttcatcaa cttctgattt aactactacg ccttgcataa agcctgttcc atcacgtaat
     6901 tgtaaaaagg cgattttacc acttgaacgt ttatttgtta accaagcacc aattgtaacg
     6961 tcttggttta aatgatcttt cgcttgttta atcgttgttt tcataaccat tctcctattt
     7021 attttttcgt tatacaatac tcattcattt taacaaaatc cgctttcaag ttctagaact
     7081 agactaaaag ataacgtgta aatggtaatg atttacgcac agatttgaac attaatttat
     7141 attaagacaa tacaatcatc agtttcagac aaaatatata ataaagcctc aatattatta
     7201 gtatgaaggt cgttttttgt gctaacattc aagtttaatt ataaaactca aattttgacg
     7261 accaaacaaa aattcttgta acagaacata gcacatgtca tgtcacaaga attttacttt
     7321 gtatttcact ttttcttttt ttgaatttgt cttaataatt ttccaaactg ttgaatgtcg
     7381 ccttttttct gacgataatt ttcaagtgtt tgttcaaaaa agtttttata attactgttt
     7441 atgagtcgat catcaaatga aactattatg ccgcgatcat tttcatttct aattaatctt
     7501 ccaagtcctt gtctaaaacg tgtaactgca tcaggtaata catattcctt gaaagttgaa
     7561 gtgaattcag aatccataag ccaatatttt gcattatgct tgttcataaa cggtaacttc
     7621 gctatcatca cacatttaat accatttgct tgaaaatcaa aaccttcaaa aaatgttgac
     7681 gtaccaagca gtatggcctt atcaaaatta ttaaactgtt gtactatttt ataattttgg
     7741 ttctgctgtt gtgttaatac aacataatct tcaaattctg gcaattcatt tagcatatct
     7801 tgtaccatat gcatcatttt ataactcgta aataagacta aacattttga tgacgttata
     7861 gtcgtatatt caataatata gcttacaatc gatgctacat actcatctat atttttatat
     7921 tgataagatg ctacatcact cggtataaat acacttgtat tttttgcact ttgtaacgac
     7981 gtgttaactt caaatgtatt aaagtgaaca tctttgttga ataactgttt aaaagcttca
     8041 aacgaatgat taaattttaa agtaccagat ataaaaatga gcgatttaaa tttttccaat
     8101 acttgtttcg ttaatacatc ttttacagcg taatctttca catataaacg tattgttgat
     8161 ttttgagata aatttttaat cgaaatgaaa ctagtatgtc cagcttttaa actttgttcg
     8221 atatttttaa atttatcttt taaatacaac aactgtttcc gtaatgattt aactgtttta
     8281 tgactaatgc cattgaatat ttctagcgtt ttatttaact tatcgataat cgcatgtaaa
     8341 tccttcaaaa tgtcttttgt ttcaaaagta aatacattat ggaagcgatg aatatcatca
     8401 tcataaacat cagaatcatt gataatcgta aatatcgttg agaacaattg ctcatttaac
     8461 tcatgaatct cattcatact agccttcaag ccaaaaatat caattggtgc aatatctaat
     8521 ttttccaaaa ttcgctgctt ttccagttga tcaattgcct ttaacaattt ttcattttcg
     8581 tttttaccaa tcaaaccaag ctgatattta atatcagcat aactcaactc atttgtcact
     8641 tgatttaagg catagtctgg taagcgatgt gcttcatcca ctatacaatc atcaaacaat
     8701 tgatatattg aattttcaac atcagaatga attaaatgtg catgatttgt aataccaatt
     8761 tgaatgttct gtgcatttcg cttaataaaa ttataataat gaacatcgtg acgtgccggt
     8821 acatatgttt caattttctg gtcaaaatac atcttttgac caccttttaa atttaattcc
     8881 tgtatatctc cggacggcgt ctctgtaatc caaatcagca attgcatttt caagatattc
     8941 acttcgtaat tacttgtgtc atcttttaaa atttgactaa taagccccaa tgaaatgtaa
     9001 tcacttttac ttttaatcaa tagtgcatta attttaaaat tcaacgcttc attcattgct
     9061 ggaatatctt tttctaacaa ttgactttgc agtaatttag tattggtaga aatcatgaca
     9121 tgcttcccag tttcaatatt atacatcaag gccgcaagta aatatgctaa tgatttacca
     9181 ctgcctagtg atgcttcaat cattgctttt tcactatgca tgagctgatc taatatagtt
     9241 tccgctaaat ataattgttg cggtcgatat gttaagccaa gttgatctac agctttgcta
     9301 tataaagact tcaagctgcc attataattt gttgtcggct ttttaaaatc aacttgctta
     9361 cgatagataa tctgttcgaa cttttcgtac gatttatcca atggctttgc atcatattgc
     9421 ctaaccatct caaagaaaat atcatacaaa tcgtatttca actgtttact taaataatat
     9481 aattgcttca aagtatctaa cggtaacttt tcaaattttt caaaagctaa tatcatcaat
     9541 ttagcagtag tagcggcatc ttcgtcagct cgatgggcat ttgctaaggt aataccatgt
     9601 gcctctgcta attcacttaa ttgatagctt ttatctgtag gaaaagctat tttaaagatt
     9661 tctagtgtat ctataacttt tttgggacga tattgaatat tacaatcttt aaatgccttt
     9721 ttaataaaat tcaaatcaaa atctacatta tgagctacaa aaatgcaatc ttttatctta
     9781 tcgtagattt cttgtgcaac ttgattaaaa tatggcgctt gttgtagcat attttcttca
     9841 atggatgtta acgcttgaat gaacggcgga atctctaaat ttgttctaat catagaatga
     9901 tatgtatcaa taatttggtt attgcgcaca aacgttatac caatttgaat gatatcgtca
     9961 aaatctaatt ggttgcctgt tgtttccaaa tccacaacgg cataggttgc catacccata
    10021 gctatctctc cttgctttag tgttaaaaat ctatatctgc actaattaaa cggtgtgatt
    10081 cacccgcttc atctctaaca attagatagc catcgtaatc taaatcaatt gcttgtcctt
    10141 taaactgttt atcattttct gtaaatagca acgttctatt ccaaatatta gaagctgcag
    10201 tatattcttc acgaatttca gaaaaaggta acgttaaaaa ttgattatat cttttttcaa
    10261 tttcttgaag taatatctct aaaaattgat atctatctaa tttattttta tcatgtaatt
    10321 gtatacttgt tgctctatgt ctaatacttt catcaaagtt ttctagttgt tgcgtcaaat
    10381 taatacctat accacatatt attgcttcta taccatcatt attagcaacc atttcagtta
    10441 agaaaccaca cactttacca ttatcaatat atatatcatt cggccatttc actttgactt
    10501 catcttgact aaaatgttga atcgcatctc ttatccctaa tgcaataaat aaattaaatt
    10561 tagatatcat tgagaatgca acgttaggtc ttaacacgac agacatccaa agtccttgcc
    10621 cttttgaaga actccaatgt ctattaaatc gcccacgacc tttcgtttgt tcatcactca
    10681 agataaaaaa tgaagattga tttccaacaa gtgacttttt cgcagcaagt tgtgtagaat
    10741 ctattgaatc gtatacttca ctaaaatcaa acaaagcaga actttttgta tattggtcta
    10801 ttataccttg ataccaaata tctgggagct gttgtaataa atgcccttta tgatttactg
    10861 aatctatttt acatccctct aactttaatt ggtcaatcac tttttttact gcagtgcgtg
    10921 aaatattaag tgattccgca atgctttgtc cagatatata attcggttta tttttataga
    10981 gtaattgaag tacatcttga ctatattttg acatgattat ccacccattt caaaatttca
    11041 gtttcttcgt tgcttacttt acctgttaca atcgctatct caatttgtct tagcacatct
    11101 tttaaccacg gaccactttt ggcatttaaa tgtgccataa gtacaccgcc attaaccatc
    11161 atgtcttttc tattatgcat aggtaaacga tgtaatgttt catcaatcgt ttgaaggtta
    11221 acgattaatg gttcatgtcc ttgaatatca tttgccttta acacgtctgc tgcaaccatt
    11281 acatttttaa tgagatgcgt atcataatca taaacaaaca tttttaattg ttcttttgta
    11341 ataatacttg gtaatgcatt cataatttga atatattgat tgatatcttt tacttgtcgg
    11401 ttacttagct ttaaaggctt caatgagtaa ttaatatcaa atttaactga tactatagca
    11461 atcaacaatt ctaaatcaat tgcttcagtt acattaattt gattcatatc aagatgttcg
    11521 aaatacggca tataattaaa tgctttcagc gattttaaat gattaaaact cttttcaaca
    11581 ttaatacctc gcattaattt agttagttca atcactatac gctcaattga taaaaattta
    11641 atatctgcca tttgtatacg catcgcttcg aatgtttccg ttgcaatatc aaatgataat
    11701 tgtgactgga accttaaaca tcgaatcata cgtaaagcat cttcttggaa tcgttcctca
    11761 gctataccta cagttcttat tattcgatta ttaatatctt gttgaccatc aaaataatca
    11821 tacaatttgt atgctgtatc cattgctatc gcattcatcg tgaaatctcg tcgttgcaaa
    11881 tcttcgtata aatcacgaac aaatgtaaca ccacttggtc tacggtgatc gacataatct
    11941 tcttcagccc ggaatgttgt cacttcataa ttttcatcat taaaaactac atttatcgtg
    12001 ccatgttctt tacctacagg tatcgtatga ctaaagatag attctatttc atccggcgtt
    12061 gcacttgttg tgatatctat atcatgaata tttcttccca tgacataatc tcttacagag
    12121 ccacctacat aatatgcttc aaaaccattg tcttgaattt gttctaatat aggccttgcc
    12181 tgttcaaata atgatttatc catattatta ctcgccttta cttttgttat gctcatttag
    12241 cattttttga taataatact catattgatc tgtaataagt tctgatccaa aacgttcagc
    12301 aatatctgct agcatgtttt tctgaagttt gttgtataac accttatctt caagtaatcg
    12361 gatagcatag tcactcgctg aatcacaatc acccacatct acgacaaatc cagtttcacc
    12421 atgtttaata acctctttaa ttccaccggc atttgaacca attggaacga cgcctgtttt
    12481 catagcctca agtaaagtta gtccaaagct ttctttttca cttaataata atactaagtc
    12541 agataattgg taaaattcac ttacgcaatc ttgtttccct aaaaataaaa catcctcttc
    12601 tacgtttaac tctttcgtca attgacgcat tggcactaat tcaggaccat ctccaagtaa
    12661 aattaattta ctaggtatct tttcacgtac ttttgcaaat gtttctataa tagtatctat
    12721 gcgttttact tgtctaaaat tcgatacatg tattaacact ttttcatctg gtgctatacc
    12781 aaattgtgat tttaatgctg tgttatgttt agttggaaac tcattttcac gtacaaaatt
    12841 ataaatcggt ataatttctt tgttagtttc aataatttca tgtgtttctt gtgctaaaga
    12901 tttactcaca cttgtcacaa tatcactttt ttcaatgcca aatttaattg caccttggag
    12961 tgaatgatca tagcccaaaa cagtaatatc agtaccgtgt agcgttgtca taatttttat
    13021 atctttacct gacatctcac gagctaaaat cccacaaatt gcatgaggta cagcatagtg
    13081 catatgcaac aaatcaagat catattcttt aataacttca gcgattttag tacttaacgt
    13141 aatatcatac ggtggatact gaaatactgc atattgattc acttcaactt gatgaaaaat
    13201 catattcggt aatggttttc ttattctaaa cgggatattt gaagtgataa aatgtacttc
    13261 gtgacctcgc tctgctaatt taattcctaa ttctgtggca ataattccag aaccacccat
    13321 ggacgggtaa catgttatac ctatcttcat tcgcttgccc atcctttctt tctatttctc
    13381 tctattattc tcgatgcgta gataattgtt taaatttaag tttatagtaa tgttgagttt
    13441 ataatttcat atatctaaaa acaggtgttg tatatataat cattcatcta gttatactta
    13501 ctttaaaaat aatataattt catgcgatgc aattcattga tggatgtttt taatcttaat
    13561 caaatccaaa taaagcatat atttttaaat tcactttctt tcgaatcgat ttttatctct
    13621 tgtattaaac ttttccattg tttcattaaa gctctctgtc atatctattc ccattgaatt
    13681 cgctaaacat aacaacacaa ataaattatc acctaattct gctttaatcg tatttgcttc
    13741 ctctgaatct ttcttctttt tttcaccata ggtatgattt atttcacgtg caagttcgcc
    13801 cacttcttca gtcaatctag ctaagttagc taatggtgaa aaatatcctg ttttaaattg
    13861 tccaatatat tcatcaactt cacgttgcat ttctaccatt gatttcattt ctacgttctc
    13921 cttatattgc atttctaata tagtatatat caatttgaag tctcatgcat gtttagtgca
    13981 atctactgac tctagctaaa ctagatgaca agttgtttat tttagaagtt taataattca
    14041 gtttatataa atgtaatgca ttcctaacta aattaaatca attgaaattg ggattataac
    14101 tttatgatac gtaccactac aataaaataa tatagtgaat aatctaccat tagaaaaata
    14161 agcacaaaaa aactagcaac cacacaaaaa tgtgattagc tagttaataa gtgtctaatt
    14221 taagttaatt gttaatctat aagattaatc acttgaacgc gcaatcaaaa taatacgtac
    14281 aagctctgct acagcgactg cagttgctgc aacataagtc attgctgctg cagataatac
    14341 tttacgcgca tgcttgtatt ctttttcatt tacaatgttc aatgccgtaa tttgtttcat
    14401 cgctcttgaa ctcgcatcaa actcaactgg taacgtaaca attgagaata ataccgctaa
    14461 tgacattaaa ccagcaccaa tccataaagc agttgaacca aatgcactac ctatcgctgt
    14521 taagataata cctaacatga tgatcatata acttaatgaa ctccctaggt ttgcaacagg
    14581 tactaatgct gctctgaatc ttaagaacca atatccttgg tgatcttgaa tggcatgacc
    14641 aacttcgtgg gctgcaattg cagttccagc aactgatggt ctgtcatagt ttgcaggaga
    14701 tagtgaaaca actttctttt taggatcgta atgatctgtt aagaatcctt cacctttaac
    14761 aacttcgaca tcataaatac cgtttgcatg taaaatttct aatgcaactt cacgacccgt
    14821 tttaccacta gttgatctaa cttgtgaata tttctcatag ttagatttaa ctttgtgttg
    14881 tgcccataaa ggaagcacca ttaatattac gaaataaatt atcatagtaa aaattgaaga
    14941 caataaactc actctccttt ataaatattt tactgtcatt tgccgttttt atcaaatcat
    15001 ttacacttta ataatttgtt taattcaata taaagcaaaa gtccaaaaac acttagacaa
    15061 catgataata caccaatttg ccacacatgt gtagttataa aatcataata tggaaattga
    15121 aggtgaaaat agtcaatata atcattcaaa aacacccaaa tcatcgctac actgattcca
    15181 atcatagaac gtttaaacct aggatagaag taaattgcct gaacagccat tatactgtgg
    15241 gaaaacatta ataccaaacc atttactgta atatcacctt gttcaataat aaataatata
    15301 ttcattataa ctgcccaaat cccatatttg aataatgtta caaatgccag tgcatcgata
    15361 atactatttt gtttttgaat taatatcaat gagatagaaa taactaagta taatattgca
    15421 gttgggctat ctggaacaaa aatcttaaaa tgccagggcg tatgacttaa ttgttcacca
    15481 taccatatat aaccataaat catccctaat atattacaaa tgagtagcat cattaaccaa
    15541 gaacgttgat aaagtgtata ttgccaaaat gctttaattg tcatctgcta agtcctcaaa
    15601 ttgattatgt ttatttacta gcttgagtgt atttaaaatt tgcgttagtt gataaaaacg
    15661 ttgcttttca ttcatctgta aacttaaatc aatattgtgt aacaagtaat ctattaataa
    15721 cgcatgttta tgccgatcta tagccatact atttaagtca tgaagataag tttgataact
    15781 gggcgattcc gttagttgag caactattaa atcatctagc ctttgctcac gctttgacac
    15841 gtttgcgaag tgaatttgaa tatcaaaagc acagttatga ttagcgatat aatcaaatat
    15901 ttcatttgta ttcattaact ttatattacg cttagtaaat tgaattgcag aagcgtgact
    15961 tcccacttct gcaatttcta atgtttcatg atgattaatt tttgtatcta caaaatgaat
    16021 gtttgccaat ttcgcctcat tcacttttat atagttaagc acccaaactg caatacgcga
    16081 cttaaatcga tattgaaaaa gtaaatattc aataaaactt tctttaattt gattgaatgt
    16141 ctctgacatc aaatacccca ttttaagatt gcaatcttga taattcgtca tgccaatttt
    16201 cgttacttgg ctctagttcc aacaattgat ttaaaatagt aattgcttgt tccttttgac
    16261 caatttcaat taaatagaaa taataatcac tcataaaatc aatatttgtt ttcatcgttg
    16321 gatatgctaa ttcaaagaaa tgttgagctt ctttatctcg ctcttcttga ccataggcga
    16381 acgctaaatg ccacatgaat gtaggatcca aatcttcttc atctacatat gttaataatt
    16441 caatgattgc ttcataatct tcttcattac gatataaatc gcttaaaatc aataaaggtt
    16501 cttggtaagc attatcaacc tctaatgctt gttttaacaa taatacacct tcattagcat
    16561 cgccgtgttc tatttccaaa catccagttg tatacattaa ctctttataa aattgactta
    16621 gtcgtaatcc ttctttaccc gtttcaatgg catctggata atttttttca ttttcatata
    16681 atgattgtaa atacaagtag ccttgaatat aatcaggatc tttagaaagt aatgtagtca
    16741 ttattttaat tgcttcttga gtgatgtcat ttttatcgta agaaatggct tttttgagat
    16801 aatcttctga agtcatttca tcttcattaa tttcatcgta taagcgaatc gcatcactat
    16861 agttaccact ttgtaaacta caatctgcca tacgagagaa taagtttaca ccattaactt
    16921 gatattcacc agtttctaaa acggtttcgt attcagaggt agcacgtaaa tattgaccat
    16981 cataatataa catttcagcc aatgcaaaat ggattattgg atcatttggc tctagttcaa
    17041 gtgcttcttg taatttatca atagcaactt ccatcatatt aatttgttga tataaatctg
    17101 cttctaacat caacttttca ggtgatggtt caacataact taaatattct aacgcttcgt
    17161 cagtttgatt ttcagacatt aaaccttcaa taaaataaat cagcaattca ctttcgtctg
    17221 gatatttgtg atataacacg cggaatactt ccaaaccttg tggcattaat ccaaaattgt
    17281 aaagtgtctc tcctagaata aataatgcgt catcgttgtc agtagttatt gcttcattaa
    17341 cacgagagtc taaattttct agtttttgta gattgatatc gtctattaat ttatagatat
    17401 cttccatttt atattatccc tcattttcta aaagctttag ttttggtaaa aatcctggaa
    17461 atgatacatt tacagcatca aattgtttga ttttgacagg ctcgcttgaa agtagagaag
    17521 caactgcaag catcattcct attcgatgat cagttaaact atcaactgtt gcatttgttt
    17581 taaattctga cggatgaata atcaatccat cattagttgg ttgtaattca aaccctaaca
    17641 agtttaacat atcagccgtt gtatcaattc tatttgtttc ttttactttt aattcctcgg
    17701 catctttaat tgtactcgtg ccaactgctt gtgtacaaag taatgctatt acaggcagtt
    17761 catcaattgc ttttggaact aattctcctt cgattgttat tggttgaagc attggtgtgt
    17821 attgaatacg aatagaagca gtaggttcag caccagttgt ttgattgaaa agttggatat
    17881 taccgcccat tttttcaaca atatcaataa tacctgaacg tgttggattg attccaacat
    17941 tatgaattgt tacatcactt cctggtgtga taagtgctgc aacaataaag aacgctgcag
    18001 atgaaatatc gccaggaaca tgaaaatctg caggtttaat gtatcgaatt gcttcagggg
    18061 ttgtattaat tgataaccct tctgcttcaa ttggaatatt aaaatgtttg aacatcgtct
    18121 cagtatgatt tcgacttaca tctaattctt taatgatggt cggttcctta gaaaacaaac
    18181 ttgcaaataa aatggcactt tttacttgtg cacttgcaac ttccatttga taatttatac
    18241 cttttatgac agatggctta ataattaatg gtgtataatt atcttcaata ccttcaatat
    18301 tcgcatccat aagtttcaat ggtctcaaga cacgatccat tggcctttta ccaattgaaa
    18361 catcgccaga caaaacactt tcaataccta aaccacttaa caaacctgcc aataatcgtg
    18421 ttgtcgtacc agaattacct gtatacaata cttgatgtgg cgtgttaaaa gattgatatc
    18481 ctggggaagt cacaactaat ttttcatcat cttctttgat ttctacacct aacagtcgga
    18541 aaatgtccat cgtacgacga caatcttcgc caagtagtgg cttatatata gtagatacac
    18601 cttcagctag cgacgccaac atgattgcac ggtgtgtcat tgacttatcg cccggcactt
    18661 ctatttcgcc ctttaacgga cctgaaatat caatgatttg ttcatttacc atttcattca
    18721 cctacttaaa atatgttttt aattgttcac atgcatgttg taatgttagt tgatcaacat
    18781 gttgtacaac gatatctcca aattgtctaa tcaagaccat ttgtacacct tgcttatcat
    18841 tctttttatc acttagcata tattggtata acgtttcaaa atccaagtca gttatcatgt
    18901 ctaaaggata gccgagttgt attaaatatt gaatataatg attaatatca tgcttagaat
    18961 caaacaaagc attcgcaact ataaattgat agataatgcc aaccatcact gcatgaccat
    19021 gaggtatttt atgatagtat tcaacagcat gaccaaatgt atgacctaaa tttaaaaatt
    19081 tacgtacacc ttgttctttt tcatctgcaa taacaatatc cagcttcgtt tcaatacctt
    19141 tagcaatata tttatccata ccatttaatg actgtaatat ctctctatct ttaaagtgct
    19201 gttcgatatc ttgcgtcgct gattcaccat tcaataacgc atgcttataa acttctgcat
    19261 agccacttaa tatttgctca aatggtaacg tctttaaaaa gtctaaatca taaatcacag
    19321 cagttggacg ataaaatgca ccgataaggt ttttaccttg ctttgagtta atacccactt
    19381 taccgccaac actagaatca tgcgctagta tagtcgttgg cacttgtata aagtgaacgc
    19441 ctcgtaaaag tgtcgccgca ataaacccag caaaatcacc agttgcacca ccaccaacag
    19501 caataattgc tgtattacga gttacatgat gggataaaat atactctaat gtttcttgat
    19561 attgctcaaa tgttttcgtc ttttcaccag ctggaataat aactttatgt acattttcat
    19621 atgataaaat atcatcaaat ttatcagcaa aatattgatt tacatgctcg tcaattaata
    19681 taaaactttg atcaaactga tcaatatacg tgctaatatg gtcaattgca ccgtgttcaa
    19741 catatattgg ataattattt gaagggtatg ttgtttgtaa tttcatgatt acacctcaat
    19801 tgttcttgtt gttaaaactc aatatttaat tgtctgcgct caataatttg ttgtttaagt
    19861 tgctcaatat gatttgattg gaattcttcc aataatgctt ttgctatttc aaatgctacg
    19921 acatgttcgc agacgatact tgctgcagga acagcacaac tatcagaacg ttcaattgtt
    19981 gctttaaagt cttctttagt attaatgtct actgaattta atggtttata taacgttgga
    20041 attggtttca ttacaccatt aacgataatt ggcattccat ttgacatacc gccttctaaa
    20101 ccacctaagt gattagatcc acgataataa ccaatttcac tattatatag aatttcatct
    20161 tgaatctcac tacctggctt ttcagctgct ttaaatcctt caccaaagct tacaccttta
    20221 aaagcattta tgctgacaac accttgtgca atcttaccat ctaacttacg atcataatgc
    20281 acataactac ctacaccaac aggcatattt tcaactacaa cttgaacgac accgccaatt
    20341 gaatctcctt catttttagc ttcgtcaatt ttatctcgca ttgcttgtgc gatactgtca
    20401 tcaattacac gaacatcatt acgatcaaga tttgctttaa atgtttctga atcataaaaa
    20461 tctttatctt taattccacc tatttcaaca acacgactgt atatatcgat atctaactgt
    20521 tgtaataaca ctttacataa ggcaccgact gcaactcgag ctgctgtttc tctagcagat
    20581 gatcgctcta gcacatttcg taaatcacga tgattatatt tcatacctcc aaccaaatct
    20641 gcatgaccag gtcttggttt tgtaatagta cgtttcatat tttcacgttc ttcttcactt
    20701 attggagctg ctcccataat ttttctccaa tgcgtaaagt catcattggt tacaaccata
    20761 gtaattggac tacctaatgt ataaccattt ctaacgcctg atactatttc tactgtatct
    20821 ttctcaattt gcatacgacg tccacgtccg taaccgcctt gacgcttaaa catttcttta
    20881 ttaatatcct caaccttaat ttctatattt gcaggtacac cttcaacaat aactgttaat
    20941 tgaggtccat gtgattctcc tgatgttagg tatctcatac acactcgctc ctttcatact
    21001 tccacgataa cattataata tgttgttaat cttatcatat tcattgtgca ttcataaatg
    21061 tttgttttca agctcaatta agaaaatgga tgacatctca ttttaaatgt cagaattatc
    21121 ttaatattaa acgaactaaa attaattata atatattcgt ctgtagaaaa gtatcatgaa
    21181 tttctaaaca gttgcatcat ataatcatta attaaaaatg attagccgtt ttaatataat
    21241 cgtatcgcaa ctttagcaaa taatatactg actacatttc ttaatacaaa agaacgaact
    21301 gttaattatt aataaagtta atcagtccgt ttaaaaaaat tatgatatta aaatatacat
    21361 atgttaagcg cttttgttat actttgactt caaaaatat
//
LOCUS       DALAGD010000052        21032 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.052, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000052 DALAGD010000000
VERSION     DALAGD010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 21032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21032
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.052"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..1586
                     /locus_tag="O2701_001532"
     CDS             <1..1586
                     /locus_tag="O2701_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR04141 family sporadically distributed
                     protein"
                     /protein_id="HCZ4293427.1"
                     /translation="KDKKLLDWQLLIKNVYLDTEEDDNLFSESGHHFDAILFLKEDTT
                     LQNNVYIIPFGQAYHDINNLIDYDFGIDFAERAIKNEDIVNKNVNFFQQNRLKEIVNY
                     RRNSVDYVRPSESYISVQGHPQNPQIFGKTMTCGTSISLRVPNRKQQFIDKISVIIKE
                     INAIINLPQKISEFPRIVTLKDLNKIEVLDTLLLKKLSNSSTTENISIDISRFLELSN
                     MILLLDDMLDVNIYIYINSFKNNTLETFDATDPEVDYITEIGDYLLKYDVNSINDVRI
                     EVIDNLGHSNNMLLKTILHAEVEMEDGKKYLLQNGKWGYFNREFFDLLNDHLNEIEIR
                     YNTLTPTGLVFKEGEEGYIKEIVGRLPEEYLMLHKKFIKPINKNFIVKGNGIELADLY
                     NIKNKELFTIKRGINTSLSLYSLEQNIIAINALKYPESYNFEELIEAIPDNSENIFND
                     IQRSTNFSIVWILPISSIDNMPIKDMVHTSNVINKNFQLTNLGSVLLKNKLVEWSLYL
                     KDQRINPIIYMETPTEDRN"
     gene            complement(1680..2132)
                     /locus_tag="O2701_001533"
                     /pseudo
     CDS             complement(1680..2132)
                     /locus_tag="O2701_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
     gene            complement(2148..2249)
                     /locus_tag="O2701_001534"
     CDS             complement(2148..2249)
                     /locus_tag="O2701_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293428.1"
                     /translation="MMKYPYMLININLSTITKNDKRFNATDDRINII"
     gene            complement(2347..3102)
                     /locus_tag="O2701_001535"
     CDS             complement(2347..3102)
                     /locus_tag="O2701_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCZ4293429.1"
                     /translation="MDIVNIVIMLLIGVFGGFISGLVGVGGAIIIYPAILLLPPLFGA
                     PAYSAYIASGLTSSQVFFSTLSGSLKARKKTEFSPQLVLYMGGGMIIGSMLGAFLANL
                     FDATFVNTVYIIIALLALTLMFIKVKPSSEKSSFNKYLLVIIGLFIGIISGIVGAGGA
                     FIIIPILLVLFKLPMNTVVANSIVIAFISSIGAFVIKLIQGYIPLYDALFLIIGSIIF
                     APIGLKLSKKVPNIIQKWIISILIVFAIVQLIL"
     gene            complement(3102..3362)
                     /gene="cstR"
                     /locus_tag="O2701_001536"
     CDS             complement(3102..3362)
                     /gene="cstR"
                     /locus_tag="O2701_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide-sensing transcriptional repressor
                     CstR"
                     /protein_id="HCZ4293430.1"
                     /translation="MNYDKKMINRINRIQGQLNGIIKMMEEGKDCKDVITQISASKSS
                     LQRLMGIIISENLIECVKAAADDEESSQELINEAVNLLVKSK"
     gene            3499..4566
                     /gene="cstA"
                     /locus_tag="O2701_001537"
     CDS             3499..4566
                     /gene="cstA"
                     /locus_tag="O2701_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide response sulfurtransferase CstA"
                     /protein_id="HCZ4293431.1"
                     /translation="MKQYGEKFIDEFSKAELEKLAKQGQLIDVRTEEEYALGHINGSI
                     LHPVDEIESFNKEKNKTYYVICRSGNRSANASKYLAKQGYNVINLDGGYKAYEEENDS
                     YDTQEEYKSIEIKADRKQFNYRGLQCPGPIVKISQEMKNIEVGDQIEVKVTDPGFPSD
                     IKSWVKQTRHTLVKLDENNNGINAIIQKEKAKDLDINYSAKGTTIVLFSGELDKAVAA
                     LIIANGARAAGKDVTIFFTFWGLNALKKVQTVNVKKQGIAKMFDLMLPKKNIRMPLSK
                     MNMFGLGNMMMRYVMKKKNVDSLPTLINQAIEQNIKLIACTMSMDVMGIQKEELRDEV
                     EYGGVGTYIGATENANHNLFI"
     gene            4597..5931
                     /gene="cstB"
                     /locus_tag="O2701_001538"
     CDS             4597..5931
                     /gene="cstB"
                     /locus_tag="O2701_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide dioxygenase-sulfurtransferase CstB"
                     /protein_id="HCZ4293432.1"
                     /translation="MFFKQFYDNHLSQASYLVGCQRTGEAIIIDPVRDLSKYIEVADS
                     EGLTITQATETHIHADFASGIRDVAKRLNANIYVSGEGEDALGYKNMPSKTQFVKHGD
                     IIQVGNVKLEVLHTPGHTPESISFLLTDLGGGSSVPMGLFSGDFIFVGDIGRPDLLEK
                     SVQIKGSTEISAKQMYESVQNIKNLPDYVQIWPGHGAGSPCGKALGAIPISTIGYEKI
                     NNWAFNEIDETKFIESLTSNQPAPPHHFAQMKQVNQFGMNLYQSYDVYPSLDNKRVAF
                     DLRSKEAFHGGHTKGTINIPYNKNFINQIGWYLDFEKDIDLIGDKSTVEKAKHTLQLI
                     GFDKVAGYRLPKSGISTQSVHSADMTGKEEHVLDVRNDEEWNNGHLDQAVNIPHGKLL
                     NENIPFNKEDKIYVHCQSGVRSSIAVGILESKGFENVVNIREGYQDFPESLK"
     gene            5950..7143
                     /gene="sqr"
                     /locus_tag="O2701_001539"
                     /pseudo
     CDS             5950..7143
                     /gene="sqr"
                     /locus_tag="O2701_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498643.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II sulfide:quinone oxidoreductase Sqr"
     gene            complement(7810..8796)
                     /locus_tag="O2701_001540"
     CDS             complement(7810..8796)
                     /locus_tag="O2701_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HCZ4293433.1"
                     /translation="MKENFWSELPRPFFILAPMEDVTDIVFRHVVSEAARPDVFFTEF
                     TNTESFCHPEGIHSVRGRLTFSEDEQPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLN
                     MGCPVANVAKKGKGSGLILRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKH
                     VFEQDIANLSIHLRTRKEMSKVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGL
                     ELAEKYGIDGVMIGRGIFHNPFAFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQF
                     KSLRRFFKIYVRGIRGASELRHQLMNTQSIAEARALLDEFEAQMDEDVKIEL"
     gene            9105..9308
                     /locus_tag="O2701_001541"
     CDS             9105..9308
                     /locus_tag="O2701_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293434.1"
                     /translation="MEGLKHSLKSLGWWDLFFAIPIFLLFAYLPNYNFITIFLNIVII
                     IFFSIGLILTTHIIIDKIKSNTK"
     gene            9357..9653
                     /locus_tag="O2701_001542"
     CDS             9357..9653
                     /locus_tag="O2701_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HCZ4293435.1"
                     /translation="MATKKDVHDLFLNHVNSNAVKRRKMMGEYIIYYDGVVIGGLYDN
                     RLLVKATKSAQQKLQDNTLVSPYPGSKEMILILDFTEATNLTDLFKTIKNDLKK"
     gene            complement(9725..10945)
                     /locus_tag="O2701_001543"
     CDS             complement(9725..10945)
                     /locus_tag="O2701_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ4293436.1"
                     /translation="MTDLLKIKNFRLFFLAEIISAFGVGISTVGANWYLIDKTNDSQL
                     LGIMLALNVLSGFLASPIIGGLADKYNRRNIILITYLLQVILYLLIVIALVMIGFETY
                     LVIGFAIVNGIGWTTYMATSRSLVKQILKPDQYTDANSLLEISLQTGMFIAGGLSGIL
                     YKINGFTLIIAMTIMMFLISIFMLFRLHVDKPTHSEEESTNSLLQEYLLGWKFLKDNM
                     MIFIFGVISIIPMVFTMIFNISLPGYVYNVLKLSSVQFGFSDMLYGIGGLCAGLISAI
                     LSKKISTKVLIFLLYFILVINSALFIWINSAFYLFIGSFILGYSISSIRIYMNTAIMN
                     TVSDKYVGRSFTIWTSISLLLQSLIAPFLGRWINEINDKFGFYIILILSLLIFVTLLL
                     VNKTGKIKYAHKEE"
     gene            complement(10938..11488)
                     /locus_tag="O2701_001544"
                     /pseudo
     CDS             complement(10938..11488)
                     /locus_tag="O2701_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498649.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
     gene            11601..12461
                     /locus_tag="O2701_001545"
     CDS             11601..12461
                     /locus_tag="O2701_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCZ4293437.1"
                     /translation="MNLNILKSFLVTSETKNLTKASELLNYSQSTVSTHIEKLERQLD
                     VKLFYRKKYGMELTEEGLAYVKYAKVILDSNSEYEREIKGLYNKKVNISINMQESQYL
                     YRYYNKISEWLAEHPYVNLKFKSAHSNFYIKEEIANFKSDISLITDEKIINSNLTAIP
                     ITEERLIFVTNKEMKDFKLNDIQNHTLLVTEKGCSYREQLEMILYKYSFSTKQMIEFL
                     GIESLKKHLKNSGGIALLPEFIVADELENKSLFQIDVDVDIPNLETTLIINPESNKQV
                     LESFVKDVFL"
     gene            complement(12816..13349)
                     /locus_tag="O2701_001546"
     CDS             complement(12816..13349)
                     /locus_tag="O2701_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4865 family protein"
                     /protein_id="HCZ4293438.1"
                     /translation="MHAMQYHVKLPSDYNMEIIRDRVRLNGYKTDGFKNLIIKAYLIS
                     QTTTNCITNTYSPLYLWRSSEGMTEFIFNGFYDNVISSFGWQNINIGVIYSMNITDSV
                     KHSLYALEEYIDIFPTLSLKEIEIKKLFRIFDNAVAEIIIYNPDKWKFVKYSFIENLP
                     KSLNPNIKIYDVLYISQ"
     gene            13440..14183
                     /locus_tag="O2701_001547"
     CDS             13440..14183
                     /locus_tag="O2701_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCZ4293439.1"
                     /translation="MIKLNFNDLEIFITVCEEASINKAAIKLRYAQSNISQRISKLEN
                     ELGVVLLFRNQKGAKATKAGEEFLAYSKKVLRDTETIKNKMKNNTMSILCSELLFNYL
                     SESEEIMMSNNSINFISSGNIRKAIEKNNYDKVISFIKINDSNYRLSNVDTMKVTLYS
                     NGSNYDKEALLINKDEFCPLRKITLDNKLDSQRVMEIDSLAAIINLVKQGKGKALLPM
                     TFENKRDIVQDISKIFEVNYYTYNHIMHH"
     gene            14617..15219
                     /locus_tag="O2701_001548"
     CDS             14617..15219
                     /locus_tag="O2701_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293440.1"
                     /translation="MLISLVIPVFALLVIGGIIWMIIEGIVHISKKNKAIDNFFNQVN
                     KVSETYKFATTFLFLILATAGISQFYLYYIVSAFLFWLVILTFGIAGIIFLMPYGLCF
                     LPFYKQKKKKQTFKKYMVYTTIGLSICLGLSLVLVHTTKIYMDEGGVRYYYGSFVMKQ
                     AGGYAYLALAVLSTLLIVAKKATNKNKEIETVDNTNITER"
     gene            15486..18638
                     /locus_tag="O2701_001549"
     CDS             15486..18638
                     /locus_tag="O2701_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA2/NAM7 family helicase"
                     /protein_id="HCZ4293441.1"
                     /translation="MSQLLNDTLSAWLLIESLSPGEVNFTAEDILSAEHFKNGAKQAQ
                     LQSFDEYFEIWNAERFIISEEKSETGELIFKFYRHCFRYNEINLKIQDIFDDYSEIHN
                     PNGTHCYGYTFNTDKHGKVIVDSIHIPMIMSALKEIEKNKNANIEEKFNDSVEKFVQK
                     VKEILADEPINEFKLKKMDKAYDEYFSVLNSKKDGLFGHYVAIEYVKDSDLPQPEFNS
                     FFISDIEKARKSPNQTLIDYIEGVEESQHIEVDENKEMFDKFLHPSRLPDGRWPSQTE
                     FRLSLMQQLAVNQITSGNERISSVNGPPGTGKTTLLKDIFAHLVVERGKELAKLNNPK
                     DAFVKTKIHETDDKYVYLLKESIAKYKMVVASSNNGAVENISKDLPKIEEIIRNPEKC
                     KFPKYEQNYANLAHELKDFAEIAEDLIGESAWGLFSGVFGKSTNINQVLSHMLKQDAN
                     DIGFAKLLQNENNRMSYNELMSEWQSHQRAFLEELRHVEMLKEESIRAYDVYKNCESF
                     SKIEQVINSEKTSIEEQVYHLDNETLRDNKEIEDLDNRINYIVKQIETLNELIKSIKE
                     SNKGFINKLKAMFNSEEDESYKDHNKEKQQLLTQQLELEKCKKNKHEDLVSKLKEKEK
                     LIKQLTKVQLQLDELNSQLQELEAYRIESKITIPEKDFWSDNNYDERQVTNLWTSDEL
                     QYRRAMLFLRAMILHKLLLIANNTTIYYAINDFKDRRKLIDANPDKVHNAWNVMHLIF
                     PVVSTTFASFKSMYGGIPKDFIDYLFIDEAGQAIPQAAVGALYRSKKVVAVGDPIQIE
                     PVVTLESHLIDNIRKNYHVPEYLVSKEASVQSVADNANQYGFWKSDATDSNQKTWIGI
                     PLWVHRRCLKPMFTIANQIAYNNKMVLPSNITKVGKTGWYDVKGNAVQKQFVKEHGEK
                     VVGLLADDWIEAIKEGKNEPSSFVISPFSAVQQQIKRMLKQQLPTRIDIERTKINQWV
                     DKSIGTVHTFQGKEAQKVYFVIGTDNTQDGAVNWSCEKPNLLNVAVTRAKKEFYVIGD
                     MQRIQMKPFYETIFKERNVK"
     gene            18695..19177
                     /locus_tag="O2701_001550"
     CDS             18695..19177
                     /locus_tag="O2701_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293442.1"
                     /translation="MEKFNNWILNAISGSQTDKNETTEELKGAKFIILYAYSMLVLLA
                     LVISNIFIHILEPKLSITTQIIIVLILIEALIGLRFLKAYDVKRGKDKENKKNSKDFV
                     KLKSILVAILFTSLALTAGTVADIYGFTDLGNTRSDLIVWSIGGIIFGLVCYTMEDKR
                     "
     gene            19382..20368
                     /locus_tag="O2701_001551"
     CDS             19382..20368
                     /locus_tag="O2701_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase C"
                     /protein_id="HCZ4293443.1"
                     /translation="MKKCIKTLFLSIILVVMSGWYHSAHASDSLSKSPENWMSKLDDG
                     KHLTEINIPGSHDSGSFTLKDPVKSVWAKTQDKDYLTQMKSGVRFFDIRGRASADNMI
                     SVHHGMVYLHHELGKFLDDAKYYLSAYPNETIVMSMKKDYDSDSKVTKTFEEIFREYY
                     YNNPQYQNLFYTGSNANPTLKETKGKIVLFNRMGGTYIKSGYGADTSGIQWADNATFE
                     TKINNGSLNLKVQDEYKDYYDKKVEAVKNLLAKAKTDSNKDNVYVNFLSVASGGSAFN
                     STYNYASHINPEIAKTIKANGKARTGWLIVDYAGYTWPGYDDIVSEIIDSNK"
     gene            20589..>21032
                     /locus_tag="O2701_001552"
     CDS             20589..>21032
                     /locus_tag="O2701_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCZ4293444.1"
                     /translation="MMKRLNKLVLGIIFLFLVISITAGCGIGKEAEVKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDQFDKNDKGTWIINSEMVIQPNNEDMVAKGMVLYMNRNTKTT
                     NGYYYVDVTKDEDEGKPHDNEKRYPVKMVDNKIIPTKEIKDEKIKK"
BASE COUNT         7849 a         2833 c         3521 g         6829 t
ORIGIN      
        1 aaaaagacaa aaaactatta gattggcaat tattaataaa gaatgtatac ctagatactg
       61 aagaagatga caatttattt tcagaatccg gtcatcattt tgatgcaata ttatttctca
      121 aagaagatac aacattacaa aataatgtat atattatacc ttttggacaa gcatatcatg
      181 atataaataa tttgattgat tatgacttcg gaattgattt tgcagaaaga gcaatcaaaa
      241 atgaagacat agttaataaa aatgttaatt tttttcaaca aaacaggctt aaagagattg
      301 ttaattatag aaggaatagt gtagattacg ttagaccttc agaatcttat atatcagtcc
      361 aaggacatcc acagaatcct caaatttttg gaaaaacaat gacttgtggt acaagtattt
      421 cattgcgtgt accgaataga aagcagcaat tcatagataa aattagtgtg ataatcaaag
      481 aaataaacgc tattattaat cttcctcaaa aaattagtga atttcctaga atagtaactt
      541 taaaagactt gaataaaata gaagtattag atactttatt gctaaaaaaa ctatcgaatt
      601 cttcaactac agaaaatata tctatagata tatcaagatt tttagaacta agtaatatga
      661 tactcttgtt agatgatatg ctcgacgtca atatatatat atatataaat tcttttaaaa
      721 ataatacatt agagacattt gatgcgactg atcctgaagt tgattacatc actgaaatag
      781 gagattactt attaaaatat gacgttaatt ctataaatga tgtcagaatt gaagtgatag
      841 ataatttggg gcattcaaat aatatgctac taaaaacgat actacatgca gaagttgaaa
      901 tggaagatgg aaagaaatat ttattacaaa atgggaaatg gggttatttt aatagagaat
      961 tttttgacct tttgaatgat catttgaatg agatagaaat taggtataac acactaactc
     1021 ccacaggttt agtatttaaa gaaggagaag aaggatatat aaaagaaata gtaggaagat
     1081 tgccagaaga atatttaatg ttacataaaa aattcataaa accaataaat aagaatttta
     1141 tagtaaaagg aaatggaata gagttggcag acttatacaa tattaaaaac aaagagcttt
     1201 tcacgattaa aagaggaatt aatacatctt tatctcttta tagtctagaa cagaatataa
     1261 tagcaattaa cgccttaaaa tatccagaat catataattt tgaagaatta atagaagcta
     1321 ttcctgataa ctcggaaaat atatttaatg atatacaacg gagtacaaat tttagtatag
     1381 tatggatttt accgatatca tctattgata atatgcccat aaaagatatg gttcatacta
     1441 gtaacgtaat aaataaaaac tttcaattaa ctaatttagg ttcagtatta cttaaaaata
     1501 aattagtaga gtggtctctc tatttaaaag atcaaagaat taatccaatt atttatatgg
     1561 aaacgccaac tgaagataga aattaaactt ttattttaaa tcaccctagt tcatacctaa
     1621 aagactttca cacaaacaaa ggaggaaact taaattcctc ctttccctta ttactcatac
     1681 tataattcaa ttttaacgtc ttcgtccatt tgggcttcaa attcatctag tagtgctcgt
     1741 acttctgcaa ttgattgtgt gttcatcaat tgatggcgaa gttcgctagc gcctcttatg
     1801 ccacgcacat agattttaaa gaatctacgc aaactcttga attgtcgtat ttcatctttc
     1861 tcatatttgt taaacaatga tagatgcaat ctcaacaaat ctaatagttc tttgcttgtg
     1921 tgttcgcgtg gttctttttc aaaagtgaat ggattgtgga aaatgcctct accaatcatg
     1981 atgccatcaa taccatattt ttctgcaagt tcaagtcctg tttttctatc gggaatatca
     2041 tcgttaattg ttaacaatgt gtttggtgca atttcgtcac gtaaattttt aatagcttcg
     2101 attaattccc aatgtgcatc tactttactc atgcgtttga taaaaactta aataatatta
     2161 attcggtcat cagtggcgtt aaatctttta tcatttttag ttatagttga taaatttata
     2221 tttataagca tatatggata tttcatcaaa aatttttatt tatataaatc cgaactgcat
     2281 acatatttgt ttaaataaga ggtattattt ttcgggaaat tgctgtctga gttaaaagga
     2341 ttagttttat aaaatgagtt gaactatagc aaaaacgatt aaaatactga taatccattt
     2401 ttgtattatg ttagggactt ttttacttaa ttttaaccct attggagcaa atataatact
     2461 ccctattata aggaataagg cgtcatataa agggatataa ccttgaataa gtttgatgac
     2521 aaaagcacca attgaagata taaaagcaat tactatacta ttagcgacta cagtattcat
     2581 tggtaatttg aataaaacca ataatatagg aataataatg aaggcaccac ctgcacctac
     2641 tatacctgaa ataataccaa tgaaaaggcc aatgataact aataaatatt tattaaatga
     2701 agacttttcg gaactaggtt tcactttaat aaacattaat gttaatgcaa gtaaagcaat
     2761 aatgatatat accgtattta caaatgtagc atcaaataaa tttgctagaa atgcacctaa
     2821 catactccct ataatcatgc cgccacccat ataaagaact aattgtggcg agaactctgt
     2881 tttttttcga gcttttaatg agccacttaa tgtactgaaa aagacttggc tagaagtaag
     2941 acctgatgcg atatatgcgc tatatgcagg ggctccgaat aatggtggta ataataaaat
     3001 agctggataa ataatgatag caccacctac gcctactaga ccagatatga acccaccgaa
     3061 taccccaatg agtaacatga taactatatt aacaatatcc attacttact tttcaccaat
     3121 aagtttacag cttcattaat taactcttgg gagctttctt catcatccgc agctgctttt
     3181 acacattcta ttaaattctc actaataatg atacccatca agcgttggag tgaactcttt
     3241 gatgcactta tttgtgtaat gacatcttta cagtcttttc cttcctccat cattttaata
     3301 attccattta gttgcccttg tattctatta atacgattaa tcattttttt atcataattc
     3361 atagtcatac ctccactttt aattgaataa aaatatatta atagataaac acaaatgtgt
     3421 caaatacccc tagaggtatt tgacaagttc catccaactg tttaaaatac ccctacaggt
     3481 atttttaggg aggttattat gaaacaatac ggagaaaagt ttatcgatga atttagtaaa
     3541 gcagaattgg aaaaactagc caagcaaggg caattaattg acgttagaac agaagaggag
     3601 tatgcattag gacatatcaa tggttccata cttcatcctg ttgatgagat tgagtcattc
     3661 aataaagaaa aaaataaaac ctattatgta atctgtagaa gtggtaacag aagtgctaat
     3721 gctagtaaat atttagctaa acaaggttat aacgttataa atcttgatgg tggttataaa
     3781 gcttatgaag aagaaaacga tagttatgat acacaagaag aatataaaag tatagaaatt
     3841 aaagcagatc gtaaacaatt taactatcgt ggtcttcaat gtccagggcc aattgtaaaa
     3901 attagtcaag aaatgaagaa tattgaagta ggtgaccaaa ttgaagtcaa agtcacagac
     3961 cctggattcc ctagtgacat taaaagttgg gtgaaacaaa caaggcatac tttagttaag
     4021 cttgatgaaa ataacaatgg aattaatgcg attattcaaa aagaaaaagc aaaagattta
     4081 gatataaatt attctgctaa aggtactaca attgtattat ttagtggaga attagacaaa
     4141 gctgtagcag cgttgattat tgcaaatggt gctagagctg ctggaaaaga tgtaactatc
     4201 ttctttactt tttgggggct taatgcatta aaaaaagtgc aaacagttaa tgttaaaaag
     4261 caaggtattg caaaaatgtt tgatttaatg ttgcccaaaa agaatatacg aatgcctctt
     4321 tccaaaatga atatgtttgg tttaggaaat atgatgatgc gctacgtaat gaaaaagaaa
     4381 aatgttgatt cattaccaac acttatcaat caagctattg agcaaaatat caaattaatc
     4441 gcttgtacga tgagtatgga tgtcatgggt attcagaaag aagaacttag agatgaagtt
     4501 gagtacggtg gtgtaggcac ttatattggt gctactgaaa atgcgaatca taatttattt
     4561 atctaattaa atctattaat aaaaggagtt gttatcatgt tttttaaaca gttttacgat
     4621 aatcatttat ctcaagcatc atatttagtg ggttgtcaac gtacaggaga ggcaataata
     4681 atagaccctg ttcgtgattt atcgaaatat atagaagttg cagattctga aggtttaaca
     4741 attacacaag ctacagaaac acatattcat gctgattttg cttcaggaat tcgtgatgtg
     4801 gctaaacgct taaatgcaaa tatatatgtg tctggcgaag gtgaagatgc attagggtat
     4861 aaaaatatgc catcaaaaac acaatttgtt aaacatggag atatcattca agtaggcaat
     4921 gttaaattag aagttctgca tactccagga cacacgcctg aaagtattag ctttttactc
     4981 actgatttag gtggtggttc aagtgttccg atgggattat ttagtggtga ctttattttt
     5041 gttggtgata taggtagacc tgatttatta gaaaaatctg ttcaaataaa gggttctaca
     5101 gaaattagcg cgaaacaaat gtatgagtcc gttcaaaata ttaaaaattt accagactat
     5161 gttcaaatct ggccgggtca tggtgctgga agcccttgtg gtaaagcatt aggtgccata
     5221 cctatatcta caataggtta tgagaaaatt aataactggg catttaatga aattgatgag
     5281 actaaattta ttgaatcatt aacatcaaat caaccagcac caccgcatca ttttgcacaa
     5341 atgaaacaag ttaatcagtt tggtatgaat ttatatcaat catatgatgt ttatcctagt
     5401 ttagataata agagagtagc atttgatctt cgtagcaaag aggcctttca cggtggccac
     5461 acaaaaggaa caatcaatat accatacaac aaaaacttta ttaatcaaat tggttggtac
     5521 ttagattttg aaaaagatat agatttaatt ggagataaat ctactgttga gaaagcgaaa
     5581 cacactttac aattaattgg gtttgataag gtagcaggct atcgtttgcc aaaatcaggc
     5641 atttcaaccc agtccgttca tagcgctgat atgacaggta aagaagaaca tgtattagac
     5701 gtacgtaatg atgaagagtg gaataatgga cacttagatc aagcagttaa tattccgcat
     5761 ggtaaattat taaatgaaaa tattcctttt aataaagagg ataaaatata tgtacattgt
     5821 cagtcaggtg ttagaagttc aattgcagtg ggtatattgg aaagcaaagg ttttgaaaat
     5881 gtggtgaata ttagagaagg ctatcaagat tttccagaat cattaaaata atttaaggat
     5941 gtggaaaaaa tgaataagca ttatcaaatt gttattattg gtggcggtac agcaggtgtt
     6001 accgtagcat caagactatt aagaaaaaat caaaacttaa aagagaaaat agcaattata
     6061 gatccagcag accatcatta ctatcaacca ttatggacgt tggttggtgc aggggtatct
     6121 agtttgaaaa gttctcgtaa agatatggaa agtgttatac ctgaaggtgc taactggata
     6181 aaacaggctg tttcaagttt ttaacctgaa aataatagcg ttattttagg agataataca
     6241 gtcgtttatt atgatttttt agtagtagct ccaggattac agattaattg gtcttcaatt
     6301 aaaggactaa aagaaaatat aggtaaaaat ggtgtttgct ctaactattc acctgactat
     6361 gttaacgaaa cttggaacca aatttctaat tttaaacaag gaaatgccat ttttacgcat
     6421 ccaaacactc ctataaagtg tggaggtgcg cctatgaaaa ttatgtattt agctgaagat
     6481 tattttagga aacataaaat ccgttctaac gctaatgtga tatatgcaac gccaaaagat
     6541 gctttatttg acgtaggaaa atataataaa gaattagaaa ggattgttga agaaagaaat
     6601 ataacagtca attataatta taaccttgtt gaaatcgacg gtgacaaaaa agtggctaca
     6661 ttcgaacata tcaaagcata cgatagaaaa acaataagtt atgatatgtt acatgtaaca
     6721 ccacctatgg gtcccttaga tgtagtaaaa gaaagtacac tttcagatag tgagggttgg
     6781 gtagatgtta acccaaccac attacagcat aaaagctact ctaatgtatt tgcacttggt
     6841 gatgcttcaa atgtacctac ttcaaaaaca ggcgcagcta ttcgtaagca agcacctatc
     6901 gtcgctaata atttattgca agtgatgaat aatcaaatgt taacgcatca ttatgatggt
     6961 tatacttcat gccctattgt tactggatat aataggttaa tacttgcaga gtttgattat
     7021 aataaaaata ctaaagaaac aatgccgttt aatcaggcca aagaacgtag aagtatgtat
     7081 atatttaaga aagatttatt acctaaaatg tattggtacg gcatgctaaa aggattaata
     7141 taataaagta cagaaaacaa taaattttta atgaaaaatc ttttactata aaagattaag
     7201 tatttaaatg acgtgtcagt gttgtgttta tatgtcgtga atttttagct ctaaatagta
     7261 taagattgaa aaagttgtta ctgttttaaa tgatcacgat gaagtcattc aataagaatg
     7321 attatgaaaa tagaaacagc agtaagatat tttctaattg aaaatcatct cactgctgtt
     7381 ttttaaaggt ttatacctca tcctctaaat tatttaaaaa taattaatgg tatttgagca
     7441 cgtttagcga ctttatgact gacattacca atttccattt cttgccagat attcaaacca
     7501 cgtgtactca aaatgatagc ttggtatgta cctccaatag taatttcaat aactttgtct
     7561 gttgaacact aagagcaatt ttaatttcat aatgtgttgt aaacattttt tttgattgga
     7621 gtttttttct gagttaaacg atatcctgat gtatttttaa ttttgcacca tttccaaaag
     7681 gataagtgac ataagtaaaa aggcatcatc gggagttatc ctatcaggaa aaccaagata
     7741 atacctaagt agaaagtgtt caatccgtgt taaattggga aatatcatcc ataaacttta
     7801 ttactcatac tataattcaa ttttaacgtc ttcgtccatt tgggcttcaa attcatcgag
     7861 tagtgctcgt gcttctgcaa ttgattgtgt gttcatcaat tgatgtcgaa gttcgctagc
     7921 gcctcttatg ccacgcacat agattttaaa gaatctacgc aagctcttga attgtcgtat
     7981 ttcatctttt tcatatttgt taaacaatga taaatgcaat ctcaatagat ctaatagttc
     8041 cttgcttgtg tgttcgcgtg gttctttttc aaaagcgaat ggattgtgga aaatgcctct
     8101 accaatcatg acgccatcaa tgccatattt ttctgccagt tcaagtcctg tttttctatc
     8161 gggaatatca ccgttaattg ttaacaatgt atttggtgca atttcgtcac gtaaattttt
     8221 aatagcttcg attaattccc aatgtgcatc tactttactc atttctttac gtgtacgaag
     8281 atgaatagat aaattggcaa tgtcttgttc gaagacgtgc ttcaaccaat ctttccattc
     8341 atcgatttca tagtagccaa ggcgtgtttt aacacttacc ggaagcccac ctgctttagt
     8401 cgcttgaata atttcggcag caacgtcagg tcttaagatt aagccggaac ccttaccctt
     8461 tttagcaaca tttgctacag gacatcccat atttaagtct atgcctttaa agcccatttt
     8521 agctaattga atactcgttt cacggaactg ttctggctta tctccccata tatgagcgac
     8581 catcggctgt tcatcttcac taaaagttaa gcgtccgcgc acactatgta tgccttcagg
     8641 gtggcaaaag ctttcagtat ttgtaaattc agtgaaaaac acatccggtc tagctgcttc
     8701 acttacaacg tgtcgaaaga cgatatctgt aacgtcttcc attggcgcca aaataaaaaa
     8761 tggacgtggt aattcactcc aaaaattttc tttcataata tatttatacc ctctttataa
     8821 ttagtatctc gattttttat gcatgatgat attaccacaa aagcctaact tatacaaaag
     8881 gaatttcaat agatgcaacc atttgaaagg gaagtctaag agtagtctaa aataaatgtt
     8941 gtggtaagtt gatcaataca aagatcaagg attatagtat taaattgttc attattaatg
     9001 atacactact tatgaatatg attcagaatt ttctttggct acttttacag taaagcgatt
     9061 ttttagttat cttataacaa agacaaattt ataaaggtga tattatggaa ggtttaaagc
     9121 attctttaaa aagtttaggt tggtgggatt tattttttgc gatacctatt tttctgctat
     9181 tcgcatacct tccaaactat aattttataa ctatatttct taacattgtt atcattattt
     9241 tcttttccat aggtttgatt ttaactacgc atataattat agataaaatt aagagcaaca
     9301 cgaaatgaat cattaatacg aatgtgatta aacataaaac tgaaggagcg attacaatgg
     9361 cgactaagaa agatgtacat gatttatttt taaatcatgt gaattcaaac gcggttaaga
     9421 gaagaaagat gatgggagaa tatattattt attatgatgg cgtggttata ggtggtttgt
     9481 atgataatag attattggtc aaggcgacta aaagtgccca gcagaaattg caagataata
     9541 cattagtttc gccatatcca ggttctaaag aaatgatatt aattctagac tttaccgaag
     9601 caacaaatct cactgattta tttaagacca taaaaaatga tttgaaaaag tgaagtagtg
     9661 aagtgtgggt gcagagagaa ctaagcccat cgataaatgg tcgcttgtta aagaagagtg
     9721 acggtcactc ttctttatgt gcatatttta ttttgcctgt tttgttaaca agcagcagtg
     9781 taacaaatat gagtaaggat aaaatgagta taatatagaa accgaattta tcattaattt
     9841 cattaatcca tcttcctaaa aatggagcaa ttaaactttg cagtaacaat gaaattgacg
     9901 tccatatcgt aaatgagcga ccgacatatt tatctgaaac agtgttcatt atagctgtat
     9961 tcatataaat tctgattgat gaaattgagt agcctagtat aaatgatcct atgaataagt
    10021 aaaatgctga gtttatccaa ataaatagtg ctgaatttat gactaatatg aaatataaca
    10081 aaaatatcaa tactttagtt gagattttct tcgaaagaat agctgaaatt aaacctgcac
    10141 ataatcctcc aatgccatat aacatatctg aaaaaccaaa ttgtacagac gaaagtttta
    10201 aaacattata aacatatcct ggtaatgata tgttaaagat cattgtaaac accattggta
    10261 tgattgaaat aactccaaaa ataaatatca tcatgttgtc ttttaaaaat ttccatccta
    10321 ataaatattc ttgcaataag ctatttgttg attcttcctc tgaatgagtt ggtttatcta
    10381 catgcaatct aaataacata aaaatgctga ttagaaacat cattatagtc atcgctataa
    10441 ttagagtgaa tccatttatt ttatataata ttcctgataa tccacctgca ataaacatac
    10501 ctgtttgcaa actaatctct aatagagaat ttgcatctgt atattgatct ggttttaata
    10561 tctgtttaac taaacttcta gatgttgcca tatacgtagt ccaacctatc ccattaacaa
    10621 tcgcaaatcc aatcactaaa taggtctcaa atcctatcat tacgagtgct atgacaatga
    10681 gtaaatataa tattacctga agaagatagg tgattaaaat aatatttcgt ctattatatt
    10741 tatcggctag tccacctatt ataggagaag ctaagaaacc ggataataca tttaaagcta
    10801 acataatacc taatagttgg gagtcgttag ttttgtcgat taaataccaa ttagccccaa
    10861 ctgtactaat tccaactccg aatgctgaaa ttatttctgc taaaaagaac aatctgaagt
    10921 ttttaatttt caacaaatca gtcataaata tcaccttttt tcaatgcaag atttatattt
    10981 gcgagctctt ctataaattg agagtcgttt aaaatatcga atggtttatt gccttcgccg
    11041 atcaaaaatc tatagttagt aatattcata aactcaaata ttaatttgaa ttggttgact
    11101 aaaggttgtg ctttggtatg cggattgtcg ccacctatga ttaaaatcaa atactttttt
    11161 tgtgacatga tttctttgaa gttgtctatt tgcgtatctc gcaatgactc ggtccatcta
    11221 tcaatgaaga gttttaaaga cgcactcatg gagtaccaat agagtggtgt tgaaaaaata
    11281 ataatatctg attctaaaac tttgattaaa atttgttcgt aatcatcatt atgaaagttg
    11341 gcacctttgc tatgacgatt atctgtaacc ttttcaatgt tgctttgata taaattcaca
    11401 aagttgactt ttaaattctt caatagattc tctactgcga tagctgaatt gccacctttc
    11461 tactacttcc aaataaacaa gtaatcatag tcataactcc tttcgattta cctttataat
    11521 aatatttaat atattattat taaatcagaa ttcttagaat tcaggattcg ataaaaggga
    11581 accctaaagg aggtactact ttgaacttaa acattttaaa atcattttta gtaactagtg
    11641 aaacaaagaa ccttactaaa gcatcagagt tacttaacta ttcacagtca actgtatcta
    11701 cacatattga aaaattagaa aggcaattag atgttaaatt attttataga aaaaaatatg
    11761 gtatggaact aacggaagaa ggcttagcat acgttaaata cgctaaagtg attttagata
    11821 gtaatagcga atacgagaga gaaataaaag gactttacaa taagaaggta aatataagta
    11881 ttaacatgca agaaagtcag tatttgtatc gctactataa taagattagt gaatggttag
    11941 ctgaacaccc atatgtaaac ttgaagttta aatccgcaca ttctaatttc tatattaaag
    12001 aagaaattgc taatttcaaa tcggatatta gccttatcac agacgaaaag attattaata
    12061 gtaacttaac tgctattcct ataactgaag aacgtttaat atttgttact aacaaagaga
    12121 tgaaagattt caaattaaac gatattcaaa accatacatt acttgttact gaaaaagggt
    12181 gtagttacag agaacagtta gaaatgattt tatataaata tagcttttca acaaaacaaa
    12241 tgattgaatt tctaggaatt gagtcattaa aaaaacactt gaaaaattca ggtgggattg
    12301 cgctattgcc ggaatttatt gttgcagatg aattagaaaa taaaagtttg tttcaaatag
    12361 acgtagatgt cgatattccg aatttagaaa caacattgat aattaatcct gaatcgaata
    12421 agcaagtact tgaatctttt gtaaaagatg tttttttata attattggtg aaaacgtgta
    12481 gttatggtga aactcaaaga taataattta aatgagatgt taatgaaaaa gtaattcaat
    12541 ataaaaacag gtgatttata tctttaataa ggattatttc tagttgaaat ttcaaattgc
    12601 gggcattcat tttcaaaata gtaaagaatt taatagatga aaacctttga atcatactag
    12661 ttagtatgga ttgattaata ttttaacctg ctaaatgagt agcttatgta ttttaaatag
    12721 ctgtcatcta gcaggttctt tattattaat aagttcagat aaattatttt agtctgtttt
    12781 ctaatgttga taataataac tttaaatctg tactttcatt gtgagatata tagtacatca
    12841 taaattttta tatttggatt gagactcttt ggaagatttt caataaatga atatttcaca
    12901 aacttccact tatcaggatt ataaattata atttctgcaa ctgcattatc gaagattcta
    12961 aacaattttt tgatttctat ttcttttaga gataaagtag ggaaaatgtc gatatattct
    13021 tcaagcgcat aaagactatg tttaacagaa tcggttatgt tcattgaata tatcacacca
    13081 atatttatat tttgccatcc aaaagaagaa ataacattat cataaaatcc attgaatata
    13141 aattcagtca tcccctctga acttctccat aaatataatg gtgaataagt atttgtaatg
    13201 caattagtag ttgtttgact tattaaataa gcttttataa ttaaattttt aaatccatca
    13261 gtcttataac catttaatcg aactctatct ctaattattt ccatattgta atcactaggc
    13321 aacttaacat gatattgcat tgcatgcatt tagcataccc ccttttataa aaaggatagc
    13381 aataataagt aaaatctcat attatccaat tgtgatatag ttatcataaa aagtgatagg
    13441 tgattaaatt gaactttaat gatttggaaa tttttataac tgtatgtgaa gaagcatcta
    13501 tcaataaagc tgcaattaaa cttagatatg cacaatctaa tatatctcaa agaattagca
    13561 agcttgaaaa tgaattaggt gtagttttgc tttttagaaa tcaaaaaggt gctaaggcaa
    13621 ctaaagcagg cgaagaattc ttagcgtata gcaaaaaagt attaagagat acagagacta
    13681 taaaaaataa aatgaaaaat aatactatgt ctattttatg ctcagaactg ttatttaatt
    13741 atttatctga gagcgaagaa attatgatgt cgaataactc aattaatttt atttctagtg
    13801 gaaatattag aaaagctata gaaaaaaata attatgataa ggttatttca ttcataaaaa
    13861 ttaacgactc aaattataga cttagtaatg ttgatactat gaaagtaacg ctttacagta
    13921 atggaagtaa ttatgataaa gaggctttac taataaataa agatgagttt tgtcctttaa
    13981 ggaaaataac tttagataat aagcttgatt cacaacgggt aatggaaatt gattcattgg
    14041 cagcaataat aaacttagtt aaacaaggaa aaggaaaggc tttattacct atgacttttg
    14101 agaataaaag agatatagta caggacattt ctaagatatt tgaagttaac tactatactt
    14161 ataatcatat tatgcatcat taattgaaag ttgattgagt ttaaattaca ggaacgattt
    14221 taaaagtcta gtagttagaa tgagcgatgt gacagaaaag aaattatgcc ttttattttg
    14281 taaattcgtt gttttaaagt aaaggtcctt gtagtatagg aaaaagtgga tgacttctcc
    14341 gcctttacca ccctttcaag cataatgata actgctaaat attgattata gcaccattgc
    14401 aaaactaatc attaattgtc atattcaact attttgaaaa gtatcaattt cacattatca
    14461 aattattgag tatctcaatt tattgttata cacaaacagt atttttgtta tcatagagtt
    14521 aataaatata ataaattaaa caagtaactt tacaaaaatt agaatgattc aattcaatca
    14581 taattgtttg aaaatagagg tgagcaggtg aacgatatgt taattagtct tgtaatccca
    14641 gtttttgctt tgttagttat tggtggtatt atttggatga ttatagaagg tatagtacat
    14701 atttcaaaaa agaataaagc aattgataac ttttttaacc aagttaataa agtaagtgag
    14761 acatataaat tcgctactac ttttttattt ctaatcttgg ctacggctgg tatttctcaa
    14821 ttttatctat attatatagt atcagcgttt cttttttggc ttgtcatact tacctttggc
    14881 attgcaggta tcattttttt aatgccatat ggattatgtt ttctaccgtt ttataagcaa
    14941 aaaaagaaaa aacagacatt taaaaaatac atggtttaca ctacgattgg tttgtcaatt
    15001 tgtctaggct tatctctagt tttggttcac actacgaaaa tttatatgga cgaaggtggc
    15061 gtaagatact attacggtag ttttgtaatg aaacaagcgg gcggttatgc ttatttagct
    15121 ttagcggtac tttcaacgtt gttaattgtt gcgaaaaaag ctacaaataa aaataaagaa
    15181 atcgaaaccg tcgacaatac aaatataacg gaaagataat taagggagtg ctcattcagg
    15241 agtgctcttt tttgatgtcc aaatttagtt gcaaatgaag gcatagaaga aatttttata
    15301 gtcatttatt ggataatgaa ttatggtcat atcgattaat tttattagtg aggattttac
    15361 aaacatttgt tgaataaata tggtaatgat aatatatgaa tatttttgaa aaaacactaa
    15421 tatgattaaa ttatttgtta aaatagcatt aagtaaaaaa caaataggga acaaggtggg
    15481 atttcatgag tcaattgcta aatgatacgt tatcggcttg gttgttaatt gaatctttaa
    15541 gtccaggaga agtaaatttt acagcggaag atatactctc agctgaacat tttaaaaatg
    15601 gtgcaaagca agcacaactt caaagttttg atgaatattt tgaaatatgg aatgctgaac
    15661 gctttattat atcagaagaa aaatcagaga ctggggaact tatatttaaa ttttatagac
    15721 attgcttccg ctataatgaa attaatttga aaattcagga tatttttgat gattattctg
    15781 agattcataa tccaaatggg acacactgtt atggttacac atttaacaca gataaacacg
    15841 gcaaagtgat agttgattct atacatattc cgatgattat gagtgcatta aaagaaattg
    15901 aaaagaacaa aaatgccaat atagaagaaa aatttaatga ttctgttgaa aaatttgttc
    15961 aaaaagtaaa agaaatttta gcagatgaac caattaatga atttaaattg aagaagatgg
    16021 acaaagctta tgatgagtac ttttctgtat taaattcaaa gaaagatgga ttatttggac
    16081 attatgtagc aatagaatat gtgaaagata gtgatttacc acagccggaa tttaacagct
    16141 tcttcataag tgatattgag aaagcaagaa aatctcccaa ccaaacttta attgattaca
    16201 ttgaaggtgt agaagaaagt cagcacatag aagtagatga aaataaagaa atgtttgaca
    16261 aatttttaca tccttcacgt ttgcctgatg gacgatggcc atcacagact gagtttagat
    16321 tgtctttaat gcaacaactt gctgtaaacc aaattacgag tggtaatgaa agaataagtt
    16381 cagttaatgg gccaccaggt acaggtaaga ctactttatt aaaagatata tttgctcatc
    16441 tagtagttga aagaggtaaa gagttagcta aactaaataa tcctaaagat gcatttgtca
    16501 aaacaaaaat tcatgaaacg gatgataaat acgtatactt actaaaggaa tctattgcca
    16561 aatataagat ggtagtcgca tctagtaata atggagctgt tgaaaatata tctaaagatt
    16621 taccgaaaat tgaagaaatt ataagaaatc ccgaaaaatg taaattccct aaatatgaac
    16681 agaattatgc aaatttagca catgaattaa aagattttgc tgaaatagct gaagatttga
    16741 ttggtgaaag tgcctggggc ttattttctg gagtttttgg taaaagtact aatattaacc
    16801 aagtattgag tcatatgtta aaacaagatg cgaatgatat tggctttgct aaattactac
    16861 aaaatgagaa taatcgtatg agttataacg agttaatgag tgaatggcaa tcacatcaac
    16921 gtgcattttt agaagagttg aggcatgttg aaatgttaaa agaagaatct attagagcat
    16981 atgatgttta taaaaattgt gagtctttct ctaagattga acaggttatt aatagtgaaa
    17041 aaacaagtat tgaagaacag gtatatcatt tagataatga aacgttacga gacaataaag
    17101 aaatagaaga tttggataat cgaattaatt atattgttaa gcaaatagaa actttaaatg
    17161 agttaattaa atccatcaaa gaaagcaaca aaggttttat taacaaactg aaagcgatgt
    17221 ttaattcaga agaagatgaa agctataaag atcataataa agagaagcaa caattattaa
    17281 cacaacagtt agagttagag aaatgtaaaa aaaacaaaca tgaagacctt gttagcaaac
    17341 taaaagaaaa agagaaatta attaaacaat taactaaagt acagttgcaa ttagacgagt
    17401 taaattcaca gttacaagag ttagaagcat atcgtattga gtcaaaaatt acaattccag
    17461 aaaaagattt ttggagtgac aacaattatg atgagcgcca agttactaat ctgtggacga
    17521 gtgacgaact tcaatacaga cgtgccatgc tctttttaag agcaatgata ttgcataaat
    17581 tattattgat tgctaataat acaactattt attatgcgat taatgatttt aaagatagaa
    17641 ggaaattaat tgatgcaaat ccagataaag tacacaacgc atggaatgtg atgcatttaa
    17701 tatttccagt agttagtacg acgtttgcaa gctttaaatc tatgtatggg ggcataccaa
    17761 aagatttcat agactactta tttattgatg aagcaggaca agcaatacct caagcagctg
    17821 tgggagcatt atatcgttca aaaaaagttg tagctgtagg tgatccgatt caaatagaac
    17881 cggttgtgac tttagaaagt catttaattg ataacattcg taaaaattat catgttccgg
    17941 aatatctagt ttctaaagaa gcttctgtgc agtctgttgc agacaacgcc aatcaatatg
    18001 gtttttggaa atctgatgct actgatagta atcaaaaaac ctggataggc atacctttat
    18061 gggtgcacag acgatgttta aaacctatgt tcacgatagc taaccaaatc gcttataata
    18121 ataaaatggt gttgccaagt aatattacaa aagtaggtaa aacaggttgg tatgacgtta
    18181 aaggaaacgc agttcaaaaa caatttgtga aagagcatgg tgaaaaagta gtgggattat
    18241 tagctgatga ttggattgaa gcaattaagg aaggtaaaaa tgaaccgagc tcatttgtaa
    18301 tatcgccttt ttcagcagta cagcaacaga ttaaacgtat gttaaagcaa caactaccga
    18361 ctagaattga tattgaacgt acaaaaatta atcaatgggt cgataaatcc attggtactg
    18421 ttcatacttt tcaaggtaaa gaggctcaga aggtgtattt tgtaataggt actgataata
    18481 cccaagatgg tgctgtgaac tggtcatgcg aaaaaccaaa cttgttaaac gttgcagtga
    18541 caagagctaa gaaagagttt tatgtaattg gcgacatgca aagaatacag atgaaaccat
    18601 tttatgagac gatttttaaa gaaagaaatg taaaataaca tacaaaaaag tatatagagg
    18661 aagttataca ttttaaaagg agcaaaattg aataatggag aaatttaaca actggatatt
    18721 aaatgcaata agtggatctc aaacagacaa gaatgaaaca actgaagaat taaaaggggc
    18781 aaaatttatc attttatatg catattcaat gctcgttttg cttgcgttag taatttctaa
    18841 catattcatt cacattttgg agcctaaact atcaatcacc actcaaatca tcatcgtttt
    18901 gattttaatt gaagcactaa ttggactgcg tttcttgaaa gcgtacgatg ttaagcgtgg
    18961 caaagataaa gaaaataaga aaaatagtaa ggatttcgtt aaactaaaat caattttagt
    19021 agcaatttta tttacatcat tggcgctgac agcaggtact gtagctgata tatacggttt
    19081 cactgactta ggaaatacta gaagtgattt aatcgtttgg agcataggtg gtattatatt
    19141 tggcctcgta tgttacacaa tggaagataa aagataacga taaggagctg gcgattataa
    19201 agctagctcc ttttttaact tatatatgta aagaactatc ctaagggttt ttaatcatat
    19261 gtcaataatt tctataatac attattaaaa catcaattaa ataagtttta aaattttaca
    19321 catattttta tttaaaaaag atgtataatt aatgtattaa atatagaaag aagttgatat
    19381 tatgaaaaag tgtattaaga ctttgttttt aagtatcatt ttagtagtga tgagtggttg
    19441 gtatcattca gcacatgcgt cagattcgtt gagtaaaagt ccagaaaatt ggatgagtaa
    19501 acttgatgat ggaaaacatt taactgagat taatataccg ggttcacatg atagtggctc
    19561 attcacttta aaggatccag taaaatcagt ttgggcaaag actcaagata aagattacct
    19621 tacccaaatg aagtcgggag tcaggttttt tgatattaga ggtagagcaa gtgctgataa
    19681 tatgatttca gttcatcacg gcatggttta tttgcatcat gaattaggaa aatttctcga
    19741 tgatgctaaa tattacttga gtgcttatcc aaacgaaaca attgtgatgt ctatgaaaaa
    19801 ggactacgat agcgattcta aagttacgaa gacatttgaa gaaattttta gagaatatta
    19861 ttataataac ccgcaatatc agaatctttt ttacacagga agtaatgcga atcctacttt
    19921 aaaagaaacg aaaggtaaaa ttgtcctatt caatagaatg gggggtacgt acataaaaag
    19981 tggttatggt gctgacacgt caggtattca atgggcagac aatgcgacat ttgaaacgaa
    20041 aattaataat ggtagcttaa atttaaaagt acaagatgag tataaagatt actatgataa
    20101 aaaagttgaa gctgttaaaa atttattggc taaagctaaa acggatagta acaaagacaa
    20161 tgtatatgtg aatttcttga gtgtagcgtc tggaggcagc gcatttaata gtacttataa
    20221 ctatgcatca catataaatc ctgaaattgc aaaaacgatt aaagcaaatg ggaaagctag
    20281 aacgggttgg ctgattgttg actatgcagg atatacgtgg cctggatatg atgatatcgt
    20341 aagtgaaatt atagatagta ataaataagg attcaataat gatattaaga cgagtatgaa
    20401 aatagttaga ttctaattat tttcactact cgtttttatt ttgaaaataa gtaataattc
    20461 aacaatatta taaattgaac agattgtttg tgaaattttt gataatatta aagtgaaaaa
    20521 gtgttataaa ttgataaata tatgtaatta acaaaaacaa atcattttaa aaagaagaga
    20581 gttgtaagat gatgaaacga ttaaacaaat tagtgttagg cattattttt ctgtttttag
    20641 tcattagtat cactgctggt tgtggcatag gtaaagaagc ggaagttaag aaaagctttg
    20701 aaaaaacatt gagtatgtac cctattaaaa atctagagga tttatacgat aaggaaggct
    20761 atcgtgatga tcagtttgat aaaaatgata aaggtacatg gattataaat tctgaaatgg
    20821 ttattcaacc taataatgaa gatatggtag ctaaaggcat ggttctatat atgaatagaa
    20881 ataccaaaac aacaaatggt tactactatg tcgatgtgac taaggacgag gatgaaggaa
    20941 aaccgcacga caatgaaaaa agatatccgg ttaaaatggt cgataataaa atcattccaa
    21001 caaaagaaat taaagatgaa aaaataaaaa aa
//
LOCUS       DALAGD010000053        20956 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.053, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000053 DALAGD010000000
VERSION     DALAGD010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 20956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20956
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.053"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            58..273
                     /locus_tag="O2701_001553"
     CDS             58..273
                     /locus_tag="O2701_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293445.1"
                     /translation="MNKKSEGLDIIIPRIFRRDHASVESLTENERRLRKEILGSIKKG
                     DYSYLEINKVFYALDRELQYRANNNKL"
     gene            298..561
                     /locus_tag="O2701_001554"
     CDS             298..561
                     /locus_tag="O2701_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCZ4293446.1"
                     /translation="MPKIIVPPTPENTYRGEEKFVKKLYATPTQIHQLFGVCRSTVYN
                     WLKYYRKDNLGVENLYIDYSPTGTLINISKLEEYLIRKHKKWY"
     gene            573..734
                     /locus_tag="O2701_001555"
                     /pseudo
     CDS             573..734
                     /locus_tag="O2701_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500084.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
     gene            827..1144
                     /locus_tag="O2701_001556"
     CDS             827..1144
                     /locus_tag="O2701_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293447.1"
                     /translation="MAENIKTEQHYYTKDFSGYRNEEDNFVANQELTVTITLNEYRKL
                     IEIKAVKDKEEDTYRGKYFAEERKNEKLEKENIKLKNKIYELQNEEDNEEDEENEEEV
                     NND"
     gene            1137..1439
                     /locus_tag="O2701_001557"
     CDS             1137..1439
                     /locus_tag="O2701_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2482 family protein"
                     /protein_id="HCZ4293448.1"
                     /translation="MTKKYKDMTQEEVKDLLSEKSGELYELAKEIKGESKFDILLFSS
                     IGVIDGDYLAGSSSVIGHTFDLASLLDSTKSYKDIVNVLQMCKSQKFHGIDDNKEG"
     gene            1444..1704
                     /locus_tag="O2701_001558"
     CDS             1444..1704
                     /locus_tag="O2701_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HCZ4293449.1"
                     /translation="MYYKFGEIKNKIISFNGFEFKVSAMKKHDGISIQIKDMNNIPLK
                     SFHVVDLSELYFATDAMRDVINEWIEENTDEQDRLINLVMKW"
     gene            1718..2080
                     /locus_tag="O2701_001559"
     CDS             1718..2080
                     /locus_tag="O2701_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293450.1"
                     /translation="MAILEDIFEELKLLNNNLRVLNTELSTVDSSIVQEKVKEAPMPK
                     EETAQLESIEEVKETSADLTKDYVLSVGKEFLKKADTSDKKEFRNKLNELGADKLSTI
                     KEEHYEKIVDFMNARINA"
     gene            2077..3243
                     /locus_tag="O2701_001560"
     CDS             2077..3243
                     /locus_tag="O2701_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HCZ4293451.1"
                     /translation="MKLDHSNRAHAKLSASGAKQWLNCPPSIKASEGIADKSTVFAEE
                     GTFAHELSELYFSLKYEGLTQFEFNKAFQNYKRNQYYSEELREYVEEYVANVEEKYNE
                     ALSRDNDVIALFETKLDLGKYVPESFGTGDVIIFSGGVLEIIDLKYGKGIEVSAIDNP
                     QLRLYGLGAYELLSLMYDIHTIRMTIIQPRIDNFSTEELPISRLLQWGADFVKPLARL
                     AYNGGGEFKAGSHCRFCKINHSCRTRAEYMQNVPQKPPHLLSDEEIAELLYKLPDIKK
                     WADEVEQYALNQAKENDKNYPGWKLVEGRSRRMITDTKAMLEKLVEAGYKPEDITETK
                     LLSITNLEKLIGKKAFSKITEGFIEKPQGKLTLATESDKRPAIKQSAEDDFDKL"
     gene            3269..3826
                     /locus_tag="O2701_001561"
     CDS             3269..3826
                     /locus_tag="O2701_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HCZ4293452.1"
                     /translation="MKAKVLNKTKVITGKVRASYAHIFEPHSMQEGQESKYSISLIIP
                     KSDTSTIKAIEQAIEAAKEEGKVSKFGGKVPANLKLPLRDGDTEREDDVNYQDAYFIN
                     ASSKQAPGIIDQNKIRLTDSGTVVSGDYIRASINLFPFNTNGNKGIAVGLNNIQLVEK
                     GEPLGGASAAEDDFDELDTDDEDFL"
     gene            3894..5846
                     /locus_tag="O2701_001562"
     CDS             3894..5846
                     /locus_tag="O2701_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HCZ4293453.1"
                     /translation="MNIDIETYSSNDISKCGAYKYTEAEDFEILIIAYSIDGGAISAI
                     DMTKVDNEPFHADFETFKIALFDPAVKKYAFNANFERTCLAKHFNKQMPPEEWICTMV
                     NSMRIGLPASLDKVGEVLRLQNQKDKAGKNLIRYFSIPCKPTKVNGGRTRNLPEHDLE
                     KWQQFIDYCIRDVEVEMTIAHKIKDFPVTAIEQAYWVFDQHINDRGIKLSKSLMLGAN
                     VLDKQSKEELLNQAKHITGLENPNSPTQLLAWLKDDQGLDIPNLQKKTVQEYLKEATG
                     KAKKMLEIRLQMSKTSVKKYNKMHDMMCSDERVRGLFQFYGAGTGRWAGRGVQLQNLT
                     KHYISDTELEIARDLIKEQRFDDLDLLLNVHPQDLLSQLVRTTFTAEEGNELAVSDFS
                     AIEARVIAWYAKEQWRLDVFNTHGKIYEASASQMFNVPVESITKGDPLRQKGKVSELA
                     LGYQGGAGALKAMGALEMGIEENELQGLVDSWRNANPNIVNFWKACQEAAINTVKSRK
                     THHTHGVRFYMKKGFLMIELPSGRALAYPKASVGENSWGSQVVEFMGLDLNRKWSKLK
                     TYGGKLVENIVQATARDLLAISIARLEASGFKIVGHVHDEVIVEIPRGSNGLKEIETI
                     MNKPVDWAKGLNLNSDGFTSPFYMKD"
     gene            5859..6044
                     /locus_tag="O2701_001563"
     CDS             5859..6044
                     /locus_tag="O2701_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3113 family protein"
                     /protein_id="HCZ4293454.1"
                     /translation="MQHQAYINASVDIRIPTEVESVNYNQIDKEKENLADYLFNNPGE
                     LLKYNVINIKVLDLEVE"
     gene            6044..6445
                     /locus_tag="O2701_001564"
     CDS             6044..6445
                     /locus_tag="O2701_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVL family protein"
                     /protein_id="HCZ4293455.1"
                     /translation="MARRKVIRVRIKGKLMTLREVSEKYHISPELLRYRYKHKMRGDE
                     LLCGRKDSKSKDEVEYMKSQIKDEEKEREKIRKKAILNLYQRNVRAEYEEERKRRLRP
                     WLYDGTPQKHSRDPYWFDVTYNQMFKKWSEA"
     gene            6445..6702
                     /locus_tag="O2701_001565"
     CDS             6445..6702
                     /locus_tag="O2701_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HCZ4293456.1"
                     /translation="MSIISNRKVDMNETQDNVKQPAHYTYGDIEIIDFIEQVTAQYPP
                     QLAFAIGNAIKYLSRAPLKNGHEDLAKAKFYVDRVFDLWEG"
     gene            6705..6905
                     /locus_tag="O2701_001566"
     CDS             6705..6905
                     /locus_tag="O2701_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293457.1"
                     /translation="MATQKQVDYVMSLQEQLELEDCEKYTDEQVKAMSHKEVSNVIEN
                     YKTSIRNEELYYECMSFGLPNC"
     gene            6920..7162
                     /locus_tag="O2701_001567"
     CDS             6920..7162
                     /locus_tag="O2701_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293458.1"
                     /translation="MTDSARKERLNQFFGSKRYLYQDNERVAHTHVVNGTYYFHGHIV
                     PGWQSVKKTFDTAEELEIYIKQHGLEYEEQKQLTLF"
     gene            7177..7425
                     /locus_tag="O2701_001568"
     CDS             7177..7425
                     /locus_tag="O2701_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HCZ4293459.1"
                     /translation="MNNREQIEQSIISASAYNGNDTEGLLKEIEDVYKKAQAFDEILE
                     GLPNAMQDALKEDIGLDEAVGIMTGQVVYKYEEDQEND"
     gene            7418..7954
                     /locus_tag="O2701_001569"
     CDS             7418..7954
                     /locus_tag="O2701_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTPase"
                     /protein_id="HCZ4293460.1"
                     /translation="MTNTLTIDQLQELLQIQKEFDDRIPTLNLRDSKIAYVVEFFEWF
                     NTLETFKNWKKKPGKPLDVQLDELADMLAFGLSIANQVGVSSEEIKEAIESSFKNSEF
                     HNMFNFKDKEFAQDAVVSTPQIIFKEFYPDQLAIVIAIDIAFNLYSIDQLIDAYKKKM
                     KRNHERQDGTADAGKGYV"
     gene            7991..8197
                     /locus_tag="O2701_001570"
     CDS             7991..8197
                     /locus_tag="O2701_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HCZ4293461.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITKAKSNQRFTVVEAESKEEAERKY
                     EAQVKRDAVIKVGQLFENIRECGK"
     gene            8194..8397
                     /locus_tag="O2701_001571"
     CDS             8194..8397
                     /locus_tag="O2701_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293462.1"
                     /translation="MTEVKIKTISDRVYYTTTDLASDDYINLVMNLVIEDFLPVKDVF
                     NNEVWVKRDEIESFTFIKEANDD"
     gene            8390..8626
                     /locus_tag="O2701_001572"
     CDS             8390..8626
                     /locus_tag="O2701_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293463.1"
                     /translation="MINIPKMKFPKKYTEIIKKYKNKAPEEKAKIEDDFIKEIKDKDS
                     EFYSPTMANMNEYELRAMLRMMPSLIDTGDDNDD"
     gene            8619..9005
                     /locus_tag="O2701_001573"
     CDS             8619..9005
                     /locus_tag="O2701_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293464.1"
                     /translation="MIKKLKNMDGFDIFIVGILSLFGIFALLLVITLPIYTVASYQHK
                     ELHQGTITDKYNKRQDKEDKFYIVLDNKQVIENSDLLFKKKFDSADIQARLKVGDKVE
                     VKTIGYRIHFLNLYPVLYEVKKVDKQ"
     gene            9002..9154
                     /locus_tag="O2701_001574"
     CDS             9002..9154
                     /locus_tag="O2701_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCZ4293465.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDYEKI
                     RAEVSW"
     gene            9222..9422
                     /locus_tag="O2701_001575"
     CDS             9222..9422
                     /locus_tag="O2701_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HCZ4293466.1"
                     /translation="MWIVISIVLAIFLLILLSSISHKMKTIEALEYMNSYLFKQLVKN
                     NGVEGLEDYENEVERIRKRFKS"
     gene            9710..12157
                     /locus_tag="O2701_001576"
     CDS             9710..12157
                     /locus_tag="O2701_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated E family protein"
                     /protein_id="HCZ4293467.1"
                     /translation="MLDKVTQIETIKYDRDVSYSYAASRLSTYWTNHNMAWSDFMQKL
                     AQTVRTKEDLTEYNKMSKSEQADIKDVGGFVGGYLKEGKRRAGQVMNRSMLTLDIDYA
                     AQDMTDILSMFYDFAYCLYSTHKHREISPRLRLVIPLKRNVNADEYEAIGRKVADIVG
                     MDYFDDTTYQPHRLMYWPSTSNDAEFFFTYEDLPLLDPDKILNEYVDWTDTLEWPTSS
                     KEESKTKRLADKQGDPEEKPGIVGAFCRAYTIEEAISTFIPDLYEKHSTNRYTYHEGS
                     TAGGLVLYENNKFAYSHHNTDPVSGMLVNSFDLVRIHLYGAQDEDAKTDTPVNRLPSY
                     KAMQQRAQNDEVVKKQLINDKMSDAMQDFDEIVNSDDAWSETLEITSKGTFKASIPNI
                     EIILRNDPNLKGKIAFNEFTKQIECLGKVPWNTNFKTRQWQDGDDSSLRSYIEKIYDI
                     HHSGKTKDAIISVAMQNAYHPVRDYLNKISWDGHKRLEKLFIKYLGVEDTEVNRTTTK
                     KALTAGIARVMEPGCKFDYMLTLYGPQGVGKSALLKKLGGAWFSDSLVSVTGKEAYEA
                     LQGVWLMEMAELAATRKAEVEAIKHFISKQVDRFRVAYGHYIEDFPRQCIFIGTTNKV
                     DFLRDETGGRRFWPMTVNPERVEVNWSKLTKEEIDQIWAEAKYYYEQGEELFLNPELE
                     EEMRSIQSKHTEESPYTGIIDEYLNTPIPSNWDDLTIFERRRFYQGDVDMLPTGNVDY
                     VERNKVCALEVFVECFGKDKGDSRGSMEIRKISNILRQLDNWSVYDGNKSGKIRFGKD
                     YGVQIAYVRDESLEDLI"
     gene            complement(12266..12364)
                     /locus_tag="O2701_001577"
     CDS             complement(12266..12364)
                     /locus_tag="O2701_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293468.1"
                     /translation="MNLMQHWKQTGLNLTARRERGVVSFVASMLHH"
     gene            12498..12788
                     /locus_tag="O2701_001578"
     CDS             12498..12788
                     /locus_tag="O2701_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HCZ4293469.1"
                     /translation="MKESTLEKYLVKEISKLNGLCLKWVAPGTRGVPDRIIIMPEGKT
                     YFVEMKQEKGKLHPLQKYVHRQFENRDHKVYVLWNKEQVNTFIRMVGGTFGD"
     gene            12769..14136
                     /locus_tag="O2701_001579"
     CDS             12769..14136
                     /locus_tag="O2701_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCZ4293470.1"
                     /translation="MEHLAIDFKPHSYQKYAIDKVIDNEKYGLFLDMGLGKTVSTLTA
                     FSELQLLDTKKMLVIAPKQVAKDTWVDEVDKWNHLNHLKVSLVLGTPKERNDALNTEA
                     DIYVTNKENTKWLCDQYKKEWPFDMVVIDELSTFKSPKSQRFKSIKKKLPLINRFIGL
                     TGTPSPNSLQDLWAQVYLIDRGERLESSFSRYRERYFKPTHQVSEHIFKWELRDGSEE
                     KIYKQIEDICLSMKAKDYLDMPDRVDTKQTVVLSEKERKVYEELEKNYILESEEEGTV
                     VAQNGASLSQKLLQLSNGAVYTDEEDVRLIHDKKLDKLEEIIEESQGQPILLFYNFKH
                     DKERILQRFKEATTLDDSNYKERWNSGDIKLLIAHPASAGHGLNLQQGGHIIVWFGLT
                     WSLELYQQANARLYRQGQNHTTIIHHIMTDNTIDQRVYKALQNKELTQEELMKAIKAR
                     IAKHK"
     gene            14149..14586
                     /locus_tag="O2701_001580"
     CDS             14149..14586
                     /locus_tag="O2701_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCZ4293471.1"
                     /translation="MGNTIYDIKPGTFKYIESEIYNLNENKKEIKRLRLEILNPTKEQ
                     DSNIVYGPLQKGEPVRTTELMATRLLTNKMLRNLEEMVEAVESEYLKLPEDHKKVIRL
                     KYWNKEKKLKMEQIGHECHMHRNTVTTIRKNFVKAVAYHAGIK"
     gene            14743..15057
                     /locus_tag="O2701_001581"
     CDS             14743..15057
                     /locus_tag="O2701_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCZ4293472.1"
                     /translation="MTKHNNIYKHGRKSYQYDWFYHSKAWKKLREIALDRDNHLCQMC
                     LREDIVTDANIVHHIIYVDEDFNKALELDNLMSVCYGCHNKIHANGNDKSNLKKIRVL
                     KI"
     gene            15184..15489
                     /locus_tag="O2701_001582"
     CDS             15184..15489
                     /locus_tag="O2701_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HCZ4293473.1"
                     /translation="MKLTKKQLKEYIEDYKKSDDILINLYIETYEFYCRLRDELKNSD
                     LMIEHTNKAGASNIVKNPLSIELTKTVQTLNNLLKSMGLTAAQRKKIVQEEGGFGDY"
     gene            15479..17170
                     /locus_tag="O2701_001583"
     CDS             15479..17170
                     /locus_tag="O2701_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCZ4293474.1"
                     /translation="MTIKVLNEPSPKLLTTWYAEQVTQGKIKTSKYVKKECERHLRYL
                     ENGGKWVFDEELAHRPIRFIEKFCKPSKGSKRQLVLQPWQHFIIGSLFGWVHKETKLR
                     RFKEALIFMGRKNGKTTTISGVANYAVSQDGENGAEIHLLANVMKQARILFDESKAMI
                     KASPKLDKNFRTLRDEIHYDATISKIMPQASDSDKLDGLNTHMGIFDEIHEFKDYKLI
                     SVIKNSRAARLQPLLIYITTAGYQLDGPLVDMVEAGRDTLDQIIEDERTFYYLASLDD
                     DDDINDSSNWIKANPNLGVSINLDEMKEEWEKAKRTPAERGDFITKRFNIFANNDEMS
                     FIDYPTLQKNNEIVSLEELEGRPCTIGYDLSETEDFTAACATFALDNGKVAVLSHSWI
                     PKHKVEYSNEKIPYREWEEDGLLTVQDKPYIDYQDVLNWIIKMNEHYVVEKITYDRAN
                     AFKLNQELKNYGFETEETRQGALTLSPALKDLKEMFLDGKIIFNNNPLMKWYINNVQL
                     KLDRNGNWLPSKQSRYRKIDGFAAFLNTYTDIMNKVVSDKGEGNIEFISIKDIMR"
     gene            17175..18413
                     /locus_tag="O2701_001584"
     CDS             17175..18413
                     /locus_tag="O2701_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCZ4293475.1"
                     /translation="MNVIAKENIVTRIKKKLIDNWIDQSASKLYDFSPWKNKSFWGVI
                     NNTLETNETIFSAITKLSNSMASLPLKMYEDYKVVNTEVSDLLTVSPNNSLSSFDFIN
                     QIETIRNEKGNAYVLIERDIYHQPSKLFLLNPDVVEMLIENQSRELYYSIHAATGNKL
                     IVHNMDMLHFKHIVASNMVQGISPIDVLKNTTDFDNAVRTFNLTEMQKPDSFMLKYGS
                     NVGKEKRQQVLEDFKQYYEENGGILFQEPGVEIEPLPKKYVSEDIVASENLTRERVAN
                     VFQLPSVFLNARSNTNFAKNEELNRFYLQHTLLPIVKQYEEEFNRKLLTKTDREKNRY
                     FKFNVKSYLRADSATQAEVYFKAVRSGYYTINDIREWEDLPPVEGGDKPLISGDLYPI
                     DTPLELRKSLKGGDKNVNES"
     gene            18397..19170
                     /locus_tag="O2701_001585"
     CDS             18397..19170
                     /locus_tag="O2701_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCZ4293476.1"
                     /translation="MSMKAKYFQMKRKSKSKGEIFIYGDIVSDKWFESDVTATDFKNK
                     LDELGDISEIDVHINSSGGSVFEGHAIYNMLKMHPAKINIYVDALAASIASVIAMSGD
                     TIFMHKNSFLMIHNSWVMTVGNAEELRKTADLLEKTDAVSNSAYLDKAKDLDQEHLKQ
                     MLDAETWLTAEEALSFGLIDEILGANEIAASISKEQYKRFENVPEDLKKDVDKITKID
                     DVDTSELVETPKESMSLEEKEKREKIKRECEILKMTMNY"
     gene            19182..20345
                     /locus_tag="O2701_001586"
     CDS             19182..20345
                     /locus_tag="O2701_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCZ4293477.1"
                     /translation="MPTLYELKQSLGMIGQQLKNKNDELSQKATDPNIDMEDIKQLET
                     EKAGLQQRFNIVERQVQDIEEKEKAKVKDKGEAYQSLSDNEKMVKAKAEFYRHAILPN
                     EFEKPSMEAQRLLHALPTGNDSGGDKLLPKTLSKEIVSEPFAKNQLREKARLTNIKGL
                     EIPRVSYTLDDDDFITDVETAKELKAKGDTVKFTTNKFKVFAAISDTVIHGSDVDLVN
                     WVENALQSGLAAKERKDALAVSPKSGLEHMSFYNGSVKEVEGADMYDAIINALADLHE
                     DYRDNATIYMRYADYVKIISVLSNGTTNFFDTPAEKVFGKPVVFTDAAVKPIVGDFNY
                     FGINYDGTTYDTDKDVKKGEYLFVLTAWYDQQRTLDSAFRIAKAKENTGPLPS"
     gene            20414..20692
                     /locus_tag="O2701_001587"
     CDS             20414..20692
                     /locus_tag="O2701_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCZ4293478.1"
                     /translation="MSLEEIKLWLRIDYNFENDLIEGLIQSAKSELLLSGVPDYDKDD
                     LEYPLFCTAIKYIIARDYESRGYSNDQSRSKVFNEKGLQKMILKLKKW"
     gene            20704..>20956
                     /locus_tag="O2701_001588"
     CDS             20704..>20956
                     /locus_tag="O2701_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293479.1"
                     /translation="MEFNEFKDRAYFFQYINKGPYPDEEEKMKLYSCFCKIYNPSMKD
                     REILKATESKSGLTIIVRSSKTEYLPQTNHLVKIDSALYS"
BASE COUNT         8106 a         2832 c         4137 g         5881 t
ORIGIN      
        1 atacttatca cctccttagg ttgataacaa cattatacac gaaaggtgga acaacaaatg
       61 aacaaaaaat cagaagggtt agacataatc ataccaagga ttttcagaag agatcatgcg
      121 tcagtagaat ctttaacaga aaatgaacgt cgactaagaa aggaaatatt aggaagtatt
      181 aaaaaaggtg attacagcta cttagaaata aacaaagttt tctatgcatt agatagagaa
      241 cttcaataca gagcgaataa taacaaactt taacatttat cgaaaggagt gatagagatg
      301 ccaaaaatca tagtaccacc aacaccagaa aacacatata gaggcgaaga aaaatttgtg
      361 aaaaagttat acgcaacacc tacacaaatc catcaattgt ttggagtatg tagaagtaca
      421 gtatacaact ggttgaaata ttaccgcaaa gataatttag gtgtagaaaa tttatacatt
      481 gattattcac caacaggcac tctgattaat atttctaaat tggaagagta tttgatcaga
      541 aagcataaaa aatggtatta ggaggatatt aaatgagcga cacatataaa agctaattaa
      601 tagcagtact gtgcttcaca gtcttagcga ttgtgcttat gccattgctg tacttcacta
      661 cagcatggtc aattgcggga ttcgcaagta tcgcaacatt catatactac aaagaatact
      721 tttatgaaga ataaaaaaac tgctacttgc gtcaacaagt aacagtgaca aacatttatc
      781 aaaatataca acttaattaa atcaaaatat acggaggtag tcaactatgg ctgaaaatat
      841 taaaactgaa caacattatt acactaaaga tttctcagga tacagaaatg aagaagataa
      901 ctttgtagca aatcaagaat tgacagtaac aatcacattg aacgagtaca gaaaacttat
      961 tgaaataaag gctgttaaag ataaagaaga agatacttac agaggtaagt attttgcgga
     1021 agaaagaaaa aacgaaaaat tggaaaaaga aaatataaaa ctaaaaaaca aaatttatga
     1081 attacaaaac gaagaagata acgaggagga cgaagaaaac gaggaggaag tcaacaatga
     1141 ctaaaaaata taaagacatg actcaggaag aagtaaaaga cttattatct gaaaaaagcg
     1201 gagaattgta tgaattagcg aaagaaatta agggagaaag taaatttgat attttgcttt
     1261 tctcatcaat aggagttatc gacggagatt atttagcagg ttcaagttct gtgattggtc
     1321 atactttcga tcttgcttcc ttattggata gcactaagag ttataaagac attgtcaatg
     1381 ttctccaaat gtgtaaatca caaaaatttc acggtattga tgataacaag gagggctaaa
     1441 acaatgtatt acaaatttgg tgagataaaa aataaaatta tcagctttaa cgggtttgaa
     1501 tttaaagtgt ctgcgatgaa aaaacatgac ggtatcagta tacaaatcaa ggatatgaat
     1561 aatattccac ttaaatcatt tcatgttgta gatttaagcg aactatattt tgcgacggat
     1621 gcaatgcgtg acgttataaa cgaatggatt gaagagaaca cagatgaaca ggacagacta
     1681 attaacttag tcatgaaatg gtaggaggat ttaatcaatg gcaatattag aagatatttt
     1741 tgaagaatta aaactattaa ataacaattt acgtgtgtta aacactgaac tatcaacagt
     1801 agattcatca attgtacaag agaaagttaa agaagcacca atgccaaaag aagaaacagc
     1861 tcaactggaa tcaattgaag aagttaagga aacttctgct gatttaacta aagattatgt
     1921 tttatcagta ggaaaagagt tccttaaaaa agcagatact tctgataaga aagaatttag
     1981 aaataaactt aacgaacttg gtgcggataa gctatctact atcaaagaag agcattatga
     2041 aaaaattgtt gattttatga atgcgagaat aaatgcatga agctagatca ctcaaataga
     2101 gctcatgcaa agcttagtgc aagtggagca aaacaatggc taaactgccc accgagtatt
     2161 aaggcaagtg aaggtattgc agataaaagt acagtttttg ccgaagaagg tacattcgcc
     2221 catgagttaa gtgagttata tttcagcctt aaatatgaag gcctaacaca gtttgagttt
     2281 aataaggctt ttcaaaatta taagagaaat caatattaca gcgaagaatt gcgtgaatat
     2341 gtggaagagt atgtagctaa tgtagaagaa aaatataacg aagctttgag tagggataat
     2401 gatgtaatag ctttatttga aacaaaatta gatttaggta aatacgtccc tgaatctttt
     2461 ggaactggtg atgtcattat attttcaggt ggtgtacttg aaattattga ccttaaatat
     2521 ggtaaaggca ttgaagtttc agctatagat aatcctcaac ttagattata tggcttgggc
     2581 gcatatgaac tgcttagttt aatgtatgac attcatacaa ttcgcatgac tatcatacaa
     2641 cctcgaatag ataacttttc tactgaagag ttaccaatat caagattact tcaatgggga
     2701 gccgattttg ttaaaccatt agccagactt gcttataacg gtggaggaga gtttaaagca
     2761 ggtagtcatt gtagattctg taagataaat cattcatgta gaacacgtgc agaatacatg
     2821 caaaatgtgc ctcaaaagcc accacatttg ttaagtgatg aagagattgc agaactttta
     2881 tataaactgc ctgatatcaa aaaatgggct gatgaagtag aacaatatgc actaaatcaa
     2941 gcgaaagaaa atgataaaaa ctatcctggt tggaagcttg tagaaggtcg ttcacgaaga
     3001 atgataactg atacaaaagc aatgcttgaa aagttagttg aagcgggtta taaacctgaa
     3061 gatattacag aaaccaagtt acttagcatt acgaatttag aaaaattaat tggtaaaaaa
     3121 gcattttcta aaattacaga gggttttata gaaaagccgc aaggtaaatt aacacttgct
     3181 accgagtcgg ataaacgacc agctataaag caatctgctg aagatgattt tgacaaacta
     3241 taaaaattta aaaggacggt atataaacat gaaagcaaaa gtattaaata aaactaaagt
     3301 gattacagga aaagtaagag catcatatgc acatattttt gaacctcaca gtatgcaaga
     3361 agggcaagaa tcaaagtatt caatcagttt aatcattcct aaatcagata caagtacgat
     3421 aaaagccatt gaacaagcta tagaagctgc taaagaagaa ggaaaagtta gtaagtttgg
     3481 aggcaaagtt cctgcaaatc tgaaacttcc attacgtgat ggagatactg aaagagaaga
     3541 tgatgtcaat tatcaagacg cttattttat taacgcatca agcaaacaag cacctggtat
     3601 tattgaccaa aacaaaatta gattaacgga ttctggaact gttgtaagtg gtgattatat
     3661 tagagcttca attaatctat ttcctttcaa cacaaatggt aataagggta tcgcagttgg
     3721 attgaacaac attcaacttg tagaaaaagg cgaacctctt ggcggtgcaa gtgcagcaga
     3781 agatgatttc gatgaattag acactgatga tgaggatttc ttataagtca ataggtgggg
     3841 ttttagcccc actttaattt taaagaaatt gaggtgtcaa gaatttgaga tttatgaata
     3901 tagatattga aacatatagc agtaatgata tttcgaaatg tggtgcctat aaatacacag
     3961 aagctgaaga tttcgaaatt ttaattatag cttattcaat agatggtgga gcgattagtg
     4021 cgattgacat gactaaagta gataatgagc ctttccacgc tgattttgag acgtttaaaa
     4081 ttgctctttt tgatcctgct gtaaaaaagt atgcattcaa tgctaatttc gaaagaactt
     4141 gtcttgctaa acattttaat aaacagatgc cacctgaaga atggatttgc acaatggtta
     4201 attcaatgcg tattggctta cctgcttcgc ttgataaagt tggagaagtt ttaagactac
     4261 aaaaccaaaa agataaagca ggtaaaaatt taattcgtta tttctctata ccttgtaaac
     4321 caacaaaagt taatggagga agaacaagaa acttgcctga acatgatctt gaaaaatggc
     4381 aacaatttat agattactgt attcgagatg tagaagtaga aatgacgatt gctcataaaa
     4441 ttaaagactt tccagtaact gcaattgaac aagcatattg ggtttttgac caacatataa
     4501 acgacagagg tattaagctt tctaaatcat tgatgttagg agctaatgtg ctcgataagc
     4561 agagtaaaga agaattgctt aatcaagcta aacatataac aggtttagaa aatcctaata
     4621 gtcctacaca attattggct tggttaaagg atgaccaagg attagatata cctaatttac
     4681 aaaagaaaac ggttcaggag tacttaaaag aagcaacagg aaaagctaaa aaaatgctag
     4741 aaattagatt gcaaatgtct aaaaccagtg tgaaaaaata caacaaaatg catgacatga
     4801 tgtgcagtga tgaacgggta agaggtctgt ttcaatttta cggtgccggt actggaagat
     4861 gggcaggtag aggtgtacaa cttcagaatt taacaaagca ttatatttca gatactgaat
     4921 tagaaatagc aagagatctt attaaagaac aacgttttga cgatttagat ttattactca
     4981 atgttcatcc tcaagactta ttaagtcaat tagttaggac gacatttact gctgaagaag
     5041 gtaatgaact agcagtaagt gatttttctg caatagaggc aagagtcata gcatggtatg
     5101 caaaagaaca atggcgttta gatgtgttca acacacacgg aaagatatat gaagcatcgg
     5161 cttctcaaat gtttaatgtc ccggtagaaa gcataactaa aggcgaccct ctcagacaaa
     5221 agggaaaagt gtccgaatta gctttaggtt atcaaggtgg cgctggagct ttaaaagcga
     5281 tgggcgcatt ggaaatgggc attgaagaaa atgaattaca aggtttagtt gatagttggc
     5341 gtaacgcaaa tcctaacata gttaattttt ggaaggcttg ccaagaggct gcaattaata
     5401 ctgtgaaatc ccgaaagacg catcatacgc atggagttag attttatatg aaaaaaggtt
     5461 ttctaatgat tgaactgcct agtggaagag ctttagctta tccaaaagct tcagttggtg
     5521 aaaatagttg gggtagtcaa gttgttgaat ttatgggctt agatcttaac cgtaaatggt
     5581 caaagttaaa aacgtatggt gggaagttag tcgagaatat tgttcaagca actgcaaggg
     5641 atttacttgc gatttctata gctaggcttg aagcatcagg ttttaaaata gttggacatg
     5701 tccatgatga agtaattgta gaaataccta gaggttcaaa tggacttaag gaaatcgaaa
     5761 ctatcatgaa taagcctgtc gattgggcaa aaggattgaa tttgaatagt gacggattta
     5821 cttctccgtt ttatatgaag gattaggagt gtgattgaat gcaacatcaa gcttatatca
     5881 atgcttctgt tgacattaga attcctacag aagtcgaaag tgttaattac aatcagattg
     5941 ataaagaaaa agagaatttg gcggactatt tatttaataa tccaggtgaa ctattaaaat
     6001 ataacgttat aaatatcaag gttttagatt tagaggtgga atgatggcta gaagaaaagt
     6061 tataagagtg cgtatcaaag gaaaactaat gacattgaga gaagtttcag aaaaatatca
     6121 tatatctcca gaacttctta gatacagata caaacataaa atgcgcggcg atgaattatt
     6181 gtgtggaaga aaagactcaa aatctaaaga tgaagttgaa tatatgaaga gtcaaataaa
     6241 agatgaagaa aaagagagag aaaaaatcag aaaaaaagcg attttgaacc tataccaacg
     6301 aaatgtgaga gcggaatatg aagaagaaag aaagagaaga ttgagaccat ggctttatga
     6361 tggaacgcct caaaaacatt cacgtgatcc gtactggttc gatgtcactt ataaccaaat
     6421 gttcaagaaa tggagtgaag cataatgagc ataatcagta acagaaaagt agatatgaat
     6481 gaaacacaag acaatgttaa acaacctgcg cattacacat acggcgacat tgaaattata
     6541 gatttcatcg aacaagttac ggcacagtat ccaccacaat tagcattcgc aataggtaat
     6601 gcaatcaaat acttgtctag agcaccgtta aagaatggtc atgaggattt agcaaaggcg
     6661 aagttttacg tcgatagagt gtttgacttg tgggaggggt aacgatggca acgcaaaaac
     6721 aagttgatta cgtaatgtca ttacaggagc aactggaatt agaagactgc gaaaaatata
     6781 cagacgaaca agttaaagca atgagtcata aagaagttag caatgtgatt gagaactata
     6841 agacaagcat aaggaatgaa gaactatatt acgaatgcat gtcgtttgga ctgcctaatt
     6901 gttaaaagga gtgacgacca tgacagatag cgcacgtaaa gaacgcttaa accaattttt
     6961 cggctctaaa agatatctgt atcaggataa cgaacgagtg gcacatactc atgtagtaaa
     7021 tggcacgtat tactttcatg ggcatatcgt gccaggttgg caaagcgtta aaaagacatt
     7081 tgatactgct gaagagctcg aaatatatat aaagcaacat ggtttggaat acgaggaaca
     7141 gaagcaacta actttatttt agaggagatg gaaatgatga ataaccgcga acaaattgaa
     7201 caatcaatta tcagtgctag tgcgtataac ggcaatgaca cagagggatt actaaaagag
     7261 attgaagacg tgtataagaa agcgcaagcg tttgatgaaa tacttgaagg tttacctaat
     7321 gctatgcaag atgcactcaa agaagatatt ggtcttgatg aagcagtagg gattatgacg
     7381 gggcaagtgg tctataaata tgaggaggat caggaaaatg actaatacat taacaattga
     7441 tcagttacaa gagttattac aaatacaaaa ggagttcgac gatagaatac caacgctgaa
     7501 cttacgagat agcaaaatag catatgtagt tgaattcttt gaatggttta atacattgga
     7561 aacgtttaag aactggaaga agaaaccagg taagccgtta gatgttcagt tagatgaatt
     7621 agcggacatg ttggcgtttg gattgagtat tgcgaatcaa gtaggagtgt catcagaaga
     7681 gataaaagaa gcgattgaat caagttttaa aaatagtgaa tttcacaaca tgtttaattt
     7741 taaagataaa gaatttgctc aagacgcagt tgttagcaca ccacagataa tattcaaaga
     7801 attttatcct gaccaattgg caattgtaat agcgatagac atagctttca acttatattc
     7861 tatcgaccag ctcattgacg catacaaaaa gaaaatgaaa aggaatcatg aaagacaaga
     7921 tggaacagca gacgcaggaa aaggatacgt gtaaagacat cttagatcga gtcaaggagg
     7981 ttttggggaa gtgacgcaat acttagtcac aacattcaaa gattcaacag gacgcaagca
     8041 tacacacata actaaagcta aaagcaatca aaggtttaca gttgttgagg cagagagtaa
     8101 agaagaagct gagcgcaaat acgaggcaca agttaaaaga gatgcagtta ttaaagtggg
     8161 tcagttattt gaaaatataa gggagtgtgg gaaatgacgg aggttaaaat taaaactatt
     8221 tcagatagag tttattacac aacaacagat ctagcttctg acgattatat taatcttgtt
     8281 atgaatctag tgattgagga ttttcttccg gtcaaagatg tgttcaacaa tgaagtatgg
     8341 gttaaaagag atgagattga atcatttaca tttattaagg aggcaaacga tgattaacat
     8401 acctaaaatg aaattcccga aaaagtacac tgaaataatc aaaaaatata aaaataaagc
     8461 acctgaagaa aaggctaaga ttgaagatga ttttattaaa gaaattaaag ataaagacag
     8521 tgaattttac agtcctacga tggctaatat gaatgaatat gaattaaggg ctatgttaag
     8581 aatgatgcct agtttaattg atactggaga tgacaatgat gattaaaaaa cttaaaaata
     8641 tggatgggtt cgacatcttt attgttggaa tactgtcatt attcggtata ttcgcattgc
     8701 tacttgttat cacattgcct atctatacag tggctagtta ccaacacaaa gaattacatc
     8761 aaggaactat tacagataaa tataacaaga gacaagataa agaagacaag ttctatattg
     8821 tattagacaa caaacaagtc attgaaaatt ccgacttatt attcaaaaag aaatttgata
     8881 gcgcagatat acaagctagg ttaaaagtag gcgataaggt agaagttaaa acaatcggtt
     8941 atagaataca ctttttaaat ttatatccgg tcttatacga agtaaagaag gtagataaac
     9001 aatgattaaa caaatactaa gactattatt cttactagca atgtatgagt taggtaagta
     9061 tgtaactgag caagtgtata ttatgatgac ggctaatgat gatgtagagg cgccgagtga
     9121 ttacgaaaaa atcagagctg aagtttcatg gtaatagcta ttatcatttt tgaattaatt
     9181 atattaatgt gtttagcaat agcactggag gtgttgtaaa tatgtggatt gtcatttcaa
     9241 tcgttttagc tatattttta ttgatcttgt taagtagcat ttctcataag atgaaaacca
     9301 tagaagcatt ggagtatatg aattcttatc ttttcaagca gttagtaaaa aataatggtg
     9361 ttgaaggttt agaagattat gaaaatgaag ttgaacgaat tagaaaaaga ttcaaaagct
     9421 aaagaggggg ctaaagccct ctccaggatt aaaagaagcg gatatttttt atttcgtttt
     9481 tgtaaattaa aactaattta tgtgaaattg tctttgaagt cttgataatt aagaaggttg
     9541 aattttctga gattatatta gttacgggaa aggcttttcc tgagtgcaat aaaattaaag
     9601 ttctcaagtt ttcattttcg tatttatcat aaatgatttc gcgtaaagtt ttcaaaattt
     9661 gtcacctctt taattttatt aaattaatta tacaagaaag gagccgaata tgttagacaa
     9721 agtcactcaa atagaaacaa ttaaatatga tcgtgatgtc tcatattctt atgctgctag
     9781 tcgtttatcc acatattgga ctaatcacaa tatggcttgg tctgacttta tgcagaagct
     9841 agcacaaaca gttagaacta aagaagattt aactgagtac aataaaatgt ctaagtctga
     9901 acaagcagat ataaaagatg ttggcggatt tgtcggtgga tatttaaaag aaggcaaacg
     9961 acgtgctggt caagtcatga atcgttcaat gctaacactt gatatcgatt atgctgctca
    10021 agatatgact gacatattat ctatgtttta tgattttgca tattgtttat attcaacaca
    10081 taagcataga gagataagtc caagactgcg tttagtgatt cctttaaaac gaaatgtaaa
    10141 tgcagatgag tatgaagcta ttgggcgtaa agtcgcagat atcgttggca tggattactt
    10201 cgatgataca acttatcaac cacataggtt aatgtattgg ccttcaacta gcaatgatgc
    10261 agaatttttc tttacctatg aagatttacc tttgttagat ccagataaaa tattaaatga
    10321 atatgttgat tggactgaca cattagaatg gccaacgtct tcaaaggaag agagtaagac
    10381 taaaagatta gcagataagc aaggtgaccc agaagaaaag ccgggaattg ttggcgcatt
    10441 ttgtagagcc tatacgatag aagaagctat atcaactttt attcctgact tatacgaaaa
    10501 acattctact aaccgttata cctatcatga aggttcaact gcaggtggat tggtgttata
    10561 cgaaaataac aagtttgcct attctcatca taatacggat cccgttagcg gtatgcttgt
    10621 gaacagtttt gatttagtac gcatacactt atatggtgct caagatgaag acgctaaaac
    10681 agatactccg gttaatcgac tacctagtta taaagcaatg cagcaaagag cgcaaaatga
    10741 tgaagttgtt aaaaagcaat taattaacga caaaatgtct gatgcaatgc aggatttcga
    10801 tgaaatagta aatagcgatg atgcatggtc tgagacgtta gaaattactt cgaaaggtac
    10861 tttcaaagct agtatcccaa atatagaaat tatattgcgt aatgatccaa atttaaaagg
    10921 aaaaatagca tttaatgaat ttacaaaaca aattgaatgc ttagggaaag tgccatggaa
    10981 tactaatttt aagacacgtc aatggcaaga cggtgatgat agcagtttaa gaagttatat
    11041 cgaaaagatt tatgacatac accattcagg taaaacaaaa gatgccatta taagcgtagc
    11101 aatgcaaaat gcttatcatc cagtaaggga ttatctaaat aaaatatcgt gggatggaca
    11161 taaacgtctt gaaaagttat ttatcaaata cttaggtgtt gaagatactg aagtgaatag
    11221 aacaactacc aaaaaagcat tgactgctgg aattgctcga gtaatggagc ctggatgtaa
    11281 atttgactat atgcttacac tttatggtcc tcaaggtgta ggtaaatctg ctttgctaaa
    11341 aaaattaggt ggtgcatggt tttctgacag tttagtttct gttacaggta aagaagccta
    11401 tgaggcctta caaggcgttt ggctaatgga aatggcagaa cttgcagcta caagaaaagc
    11461 tgaagttgaa gctattaagc atttcatatc taaacaagtt gaccggtttc gtgttgctta
    11521 tggacattat attgaagatt ttccaaggca atgtattttc attggtacaa ctaataaagt
    11581 tgatttctta agagatgaaa ctggtggaag acgtttttgg ccaatgactg taaatccaga
    11641 gagagttgaa gtgaactggt ctaaactaac caaagaagag atcgaccaaa tttgggcaga
    11701 agctaaatat tattatgaac aaggagaaga gttattcctc aaccctgaac tagaagaaga
    11761 aatgcgttca atacaaagca aacatactga ggaatctcca tatacaggca ttattgatga
    11821 atatcttaac acaccaattc ctagcaattg ggatgactta actatctttg aacgaagacg
    11881 attttatcaa ggtgatgttg atatgttacc aacaggaaat gtagattacg ttgaaagaaa
    11941 taaggtctgt gcgcttgaag tgtttgttga atgttttggt aaagataagg gagatagtag
    12001 aggatctatg gaaattagaa agatttcaaa catcttaaga caattagaca attggtctgt
    12061 atatgatggt aataaaagtg ggaaaattcg atttggaaaa gattatggtg tacagatagc
    12121 ttatgtaaga gatgaaagtt tagaagattt aatataataa atattgaata aatatacatt
    12181 ttagagtgtt gtatcagatg ttgcatcatt ttttgagtga tgcaacacgt gagtgtaaaa
    12241 agtaatcgta ggtgttgcat catttttagt gatgcaacat tgatgcaaca aatgatacaa
    12301 cacctctttc ccttctcgct gtaaggttca accctgtttg tttccaatgt tgcatcaaat
    12361 tcactataaa gtttaaaaag tagtgttagg gagtaaagag gtataggggt aaccctctaa
    12421 cagctatttt taaaagtttg gcaagaattg atgcaacatc ggaacacaaa tataaatttt
    12481 gtatacaagg tgaatatatg aaagaatcga cattagaaaa atatttagtg aaagagatat
    12541 caaagctaaa cggtttatgt ttaaaatggg ttgcacctgg aacaagaggt gtgccagata
    12601 gaattattat tatgccagaa ggaaaaacat attttgtaga aatgaagcaa gaaaaaggaa
    12661 agttgcatcc tttacaaaaa tatgtgcata gacaatttga aaatagagat cataaagtat
    12721 atgtgttatg gaataaagaa caagtaaata cttttatcag aatggtaggt ggaacatttg
    12781 gcgattgact tcaaaccaca tagctatcaa aagtatgcaa tagataaagt gatagataat
    12841 gagaaatacg gtctgttttt agatatgggt ctagggaaaa cagtatcaac acttacagca
    12901 tttagtgaat tgcagttgtt agacactaaa aaaatgttag ttatagcacc taaacaagtt
    12961 gctaaagata catgggttga tgaagttgat aagtggaacc atttaaatca tctgaaagtg
    13021 tctttagttt taggaacacc taaagaaaga aatgatgcat taaacacaga ggctgatatc
    13081 tatgtaacca ataaagaaaa tactaaatgg ttatgtgatc aatataaaaa agaatggcca
    13141 tttgacatgg ttgtgattga tgaactgtct acatttaaaa gtcctaagag tcaaaggttt
    13201 aaatctatta aaaagaaatt accactcatt aatagattta taggattaac aggaacacct
    13261 agtccaaata gtttacagga tttatgggct caagtttatt tgatagacag aggtgaaaga
    13321 cttgagtctt cattcagtcg ttatcgagaa aggtacttta aaccaactca tcaagttagc
    13381 gaacatattt ttaagtggga gctaagagac ggatctgaag aaaagatata taaacaaata
    13441 gaagatatat gtttaagcat gaaagcgaaa gattatctgg atatgcctga cagagttgat
    13501 actaaacaaa cagtagtctt atcagaaaaa gaaagaaaag tatatgaaga attagaaaaa
    13561 aactatattt tagaatcgga agaagaagga acagttgtag ctcaaaatgg ggcatcatta
    13621 agtcagaaac tacttcaact atctaacggc gcagtttata cagatgagga agatgtaaga
    13681 cttatacatg ataagaagtt agataagtta gaggaaatta tagaggagtc tcaaggccaa
    13741 ccaatactat tgttttataa cttcaaacac gataaagaaa gaatacttca aaggtttaag
    13801 gaagcaacca cattagacga ttcaaactat aaagaacgtt ggaacagtgg agacattaag
    13861 ttgcttatag cacatccagc aagtgcagga catggattaa acttacaaca aggtgggcac
    13921 attattgttt ggtttggact tacatggtcc ttggaattat accaacaagc aaatgctaga
    13981 ttatacagac aaggacaaaa tcatacgact attattcatc atatcatgac cgataacaca
    14041 atagatcaaa gagtatataa agctttacaa aataaagaac taacgcaaga agaattaatg
    14101 aaagctatta aagcaagaat agctaagcat aagtaatgga ggtctaacat ggggaacaca
    14161 atatatgata tcaagccagg cacatttaaa tatattgaat cagaaatata taatttaaat
    14221 gagaacaaga aagaaataaa aagattgagg ttggaaatac ttaatccaac gaaggaacaa
    14281 gattccaata ttgtatatgg accattacaa aaaggcgaac cagttagaac aactgaacta
    14341 atggcaacta gattattaac taataagatg ttacgaaacc ttgaagaaat ggtcgaagca
    14401 gttgagagtg aatacttaaa gttgcctgaa gatcataaga aagtaataag gctcaagtat
    14461 tggaataaag aaaagaagtt aaagatggaa cagataggac atgaatgcca tatgcatcgt
    14521 aatactgtta ctactatacg aaagaacttt gttaaagcgg tagcgtatca tgcaggtatc
    14581 aaataacatt gtgcaaagat tgtgcaaaag gcctacaaat ctgtagtaat atgatagtat
    14641 cggaaagatg tataaagtta tctaaaagtt atacgacaca agtacacgag gcacatcgct
    14701 atgcggtgtg tcttttgtta tgcaatcaaa gaggtgtaag agatgaccaa gcacaataac
    14761 atttataagc atggtcgtaa gtcatatcaa tacgattggt tctatcattc aaaagcatgg
    14821 aagaagttaa gggagatagc attagataga gataatcatc tttgtcaaat gtgtttacgc
    14881 gaagatattg taacagatgc aaacatagtg catcacatta tttatgttga tgaagatttt
    14941 aacaaagctt tagaattaga taatctaatg tcagtttgtt atggctgtca taacaaaatt
    15001 catgcaaatg gtaatgacaa aagtaatctt aagaaaatta gagttctaaa aatttaaata
    15061 aaaaaacatt taaataaaat tttatagccc cctgcccatt ggcttaaaat gttttttcgc
    15121 cgggtaccgg cgggggccct tcgcttgcaa cgcggataaa cttttatgaa agggggtctt
    15181 tatatgaaat taacaaaaaa acagctgaaa gaatatatag aggattataa aaaatctgat
    15241 gacatattaa ttaatttgta tatagaaacg tatgaatttt attgtcggtt aagagatgaa
    15301 cttaaaaata gtgatttgat gatagagcat acaaacaagg ctggtgcgag caatattgtt
    15361 aagaatccat taagcataga actgacaaaa acagttcaaa cactaaataa cttactcaag
    15421 tctatgggtt taactgcagc acaaagaaaa aagatagttc aagaagaagg tggattcggt
    15481 gactattaaa gttttaaatg aaccttcacc aaaactatta acaacatggt atgcagagca
    15541 agtcactcaa gggaaaataa aaacaagcaa atatgttaaa aaagaatgtg agagacacct
    15601 tagatatcta gaaaatggag gtaaatgggt atttgatgaa gaattagcgc accgtcctat
    15661 tcgattcata gaaaagtttt gtaaaccttc caaaggatct aaacgtcaac ttgtattaca
    15721 accatggcaa cattttatta ttggcagttt gtttggttgg gttcataaag aaacaaaact
    15781 gcgcaggttt aaagaagctt tgatatttat ggggcgaaaa aatggtaaaa caactactat
    15841 atctggtgtt gctaactatg ctgtttctca agatggagaa aacggcgctg aaatccatct
    15901 tttagcaaac gtaatgaaac aagctagaat attattcgat gaatctaagg cgatgataaa
    15961 agctagccca aagcttgata aaaatttcag aacattaaga gatgaaatcc attatgacgc
    16021 aacgatatca aaaattatgc cccaagcatc agatagcgat aagttagatg gattgaatac
    16081 acacatgggg atttttgatg aaattcatga atttaaagac tataaattga tttcagttat
    16141 aaaaaactca agagctgcaa ggttacaacc tcttctcatc tacattacga cagcagggta
    16201 tcaattagat ggtccacttg ttgatatggt agaagcggga agagacacct tagatcaaat
    16261 catagaagac gaaagaactt tttattattt agcatctttg gatgatgacg atgatattaa
    16321 tgattcgtcg aactggataa aagcaaatcc caacttaggt gtctctataa atttagatga
    16381 gatgaaagaa gagtgggaaa aagctaagag aacaccagct gaacgtggag attttataac
    16441 caaaaggttt aatatctttg ctaataatga cgagatgagt tttattgatt acccaacact
    16501 ccaaaaaaat aatgaaattg tttctttaga agagctggaa ggcagaccat gcacgattgg
    16561 ttatgattta tcagaaacag aggactttac agccgcgtgt gctacttttg cgttagataa
    16621 tggtaaagtt gcagttttat cgcattcatg gattcctaag cacaaagttg aatattctaa
    16681 cgaaaaaata ccctatagag aatgggaaga agatggctta ttaacagtgc aagataagcc
    16741 ttatattgac taccaagatg ttttaaattg gataattaag atgaatgagc attatgtagt
    16801 agaaaaaatt acttatgata gagcgaacgc attcaaacta aatcaagagt taaaaaatta
    16861 cgggtttgaa acggaagaaa caagacaagg agctttgacc ttgagccctg cattgaagga
    16921 tttaaaagaa atgtttttag atgggaaaat aatatttaat aataatcctt taatgaaatg
    16981 gtatatcaat aatgttcagt tgaaactaga cagaaacgga aactggttgc cgtctaagca
    17041 aagcagatat cgtaaaatag atggctttgc agcattttta aacacatata cagatattat
    17101 gaataaagtt gtttctgaca agggtgaagg aaacatagaa tttattagta ttaaagatat
    17161 aatgcgttaa ggaggtgaat gttatcgcaa aagagaatat tgtcacacgc ataaagaaaa
    17221 aattgataga caattggatt gatcagtcag cttctaagct ttatgacttt agcccatgga
    17281 aaaataaatc tttttggggt gtaatcaata atacgcttga aactaatgaa acgatatttt
    17341 cagctattac aaagttatct aattcgatgg ctagtttgcc cttgaaaatg tatgaagatt
    17401 ataaagtagt taatacagaa gtatctgatt tacttacagt gtcaccgaat aattctctga
    17461 gcagttttga ttttattaat caaattgaaa caatcagaaa tgaaaaaggt aatgcatatg
    17521 tgctaattga acgagacatc tatcatcaac catcaaagct tttcttatta aatccagatg
    17581 ttgttgaaat gttaattgaa aaccaatcac gtgaacttta ttattccatt catgctgcaa
    17641 ctggaaataa attgattgtt cataatatgg acatgttgca ttttaaacac atcgtggcat
    17701 ctaatatggt gcaaggcatt agtccgattg atgtgttgaa gaatacaact gattttgata
    17761 atgcagtaag aacctttaat cttacagaaa tgcaaaaacc tgattctttc atgcttaaat
    17821 atggttccaa tgtaggtaaa gaaaaaaggc agcaagtgtt agaagatttc aaacagtact
    17881 atgaagaaaa cggtggaata ttattccaag agcctggtgt tgaaatcgaa ccgttaccta
    17941 aaaaatatgt ctctgaagat atagtggcaa gcgagaattt aacaagagaa agagtagcta
    18001 acgtttttca attgccctca gtattcttaa atgcaagatc aaatacaaat ttcgcgaaaa
    18061 atgaagagtt aaacagattt tacttgcagc ataccttatt gccaatcgtc aaacagtatg
    18121 aagaagaatt taatcggaaa ctacttacta aaacagacag agaaaaaaat aggtatttta
    18181 aatttaacgt taaatcttat ttaagggctg atagtgcaac acaagcagaa gtgtacttta
    18241 aagcagttcg tagtggttac tacactataa atgacattag agagtgggaa gatttaccac
    18301 cagttgaagg tggagataag ccgctaataa gcggtgattt atacccaatt gacacgccac
    18361 ttgaattaag aaaatctttg aaaggtggtg ataaaaatgt caatgaaagc taagtatttt
    18421 caaatgaaaa gaaaatcaaa aagtaaaggt gaaatattta tttatggtga tattgtaagt
    18481 gataaatggt ttgaaagtga tgtaactgct acagatttca aaaataaact agatgaacta
    18541 ggagacatca gtgaaataga tgttcatata aattcatctg gaggcagtgt atttgaaggg
    18601 catgcaatat acaatatgct aaaaatgcat cctgcaaaaa ttaatatcta tgtcgatgcc
    18661 ttagcggcat caattgctag tgttatcgct atgagtggtg acactatttt tatgcacaaa
    18721 aatagttttt taatgattca taattcatgg gttatgactg taggtaatgc agaagaatta
    18781 agaaagacag cggatttact tgaaaaaaca gatgctgtta gtaattcagc ttatttagat
    18841 aaagcaaaag atttagatca agaacactta aaacagatgt tagatgcaga aacttggctt
    18901 actgcagaag aagccttgtc tttcggcttg atagatgaaa ttttaggagc taatgaaata
    18961 gctgctagta tctctaaaga gcaatataag cgtttcgaga acgtcccaga agatttaaag
    19021 aaagatgtag acaaaatcac taaaattgat gatgtagata catctgaatt ggttgaaaca
    19081 cctaaagaaa gtatgtcact agaagaaaaa gaaaaaagag aaaaaattaa acgcgaatgc
    19141 gaaattttaa aaatgacaat gaattattag gaggaaatga aatgccgaca ttatatgaat
    19201 taaaacaatc cttaggtatg attggacaac aattaaaaaa taaaaatgat gaattgagtc
    19261 agaaagcaac agatccaaat attgatatgg aagacatcaa acaactagaa acagaaaaag
    19321 caggtttaca acaaagattt aacattgttg aaagacaagt gcaagacatt gaagagaaag
    19381 aaaaagcgaa agttaaagat aaaggagaag cttatcaatc tttaagtgat aatgagaaga
    19441 tggttaaagc taaggcagag ttttatcgtc acgcgatttt accaaatgaa tttgaaaaac
    19501 cttcaatgga ggcacaacgt ttattacacg ctttaccaac aggaaatgat tcaggtggag
    19561 ataagctctt accaaaaaca ctttctaaag aaattgtttc agaaccattt gctaaaaacc
    19621 aattacgtga aaaagctcgt ctaactaaca ttaaaggttt agagattcca agagtttcat
    19681 acactttaga cgatgatgat ttcattacag acgtagaaac agcaaaagaa ttaaaagcaa
    19741 aaggtgatac agtcaagttc actactaata aattcaaagt atttgctgca atttcagata
    19801 ctgtaattca tggatcagat gtagatttag taaactgggt tgaaaacgca ctacaatcag
    19861 gattagcagc taaagagcgt aaagatgcct tagcagtaag tcctaaatct ggattagaac
    19921 acatgtcatt ttataatgga tctgttaaag aagttgaggg agcagacatg tatgatgcta
    19981 ttattaacgc tttagcagat ttacatgaag attatcgtga taacgcaaca atttatatgc
    20041 gatatgcaga ttatgtcaaa attattagtg ttctttcaaa tggaacaaca aatttctttg
    20101 acacaccagc agaaaaagta tttggcaaac cagtagtatt tacagatgca gcagttaaac
    20161 ctattgtggg agatttcaat tattttggaa ttaactatga tggaacaact tatgacactg
    20221 ataaagatgt taaaaaaggc gaatatttgt ttgtattaac agcatggtat gatcagcaac
    20281 gtacattaga cagtgcattc agaattgcaa aagcaaaaga aaatacaggt ccattaccca
    20341 gctaagcccc aaaaggttaa tgtaacagct aaggctaaat cagctgtaat atcagccgaa
    20401 taggggtgat gaaatgagtt tagaagaaat taaattgtgg ttgagaattg actataattt
    20461 cgaaaatgat ttaattgaag gtctcattca atcggctaag tctgaattac tattaagtgg
    20521 ggttccagat tatgacaaag atgacttgga atacccgctt ttttgtacag cgattaaata
    20581 tatcattgca agagattatg aaagtcgtgg atactcaaat gaccaatcta gaagcaaggt
    20641 gtttaatgaa aaaggattgc aaaaaatgat tttgaaatta aaaaagtggt aggtgatttt
    20701 taaatggaat ttaatgaatt taaagatcgc gcgtattttt ttcaatatat aaacaaagga
    20761 ccatatccag atgaagagga aaaaatgaaa ttgtatagtt gcttttgtaa aatttataat
    20821 ccttctatga aagatagaga aattttaaaa gcgactgaat caaaatcagg attaaccata
    20881 attgtcaggt cttctaaaac tgaatatcta ccacaaacaa atcacttagt taaaattgac
    20941 agtgcattat attccg
//
LOCUS       DALAGD010000054        20785 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.054, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000054 DALAGD010000000
VERSION     DALAGD010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 20785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20785
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.054"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            259..834
                     /locus_tag="O2701_001589"
     CDS             259..834
                     /locus_tag="O2701_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293480.1"
                     /translation="MNAKEQLVDNLMKTSSQLFKFHGEVAMQLFLNDELKLPSIVEIC
                     VERKRLSDIVKVIPQSYALLYIDKQDQAIAKEDLSLSKIAKVYVQYDDTTIMSIFVYD
                     VVNDEWIFRLDPNIRIPKSNIYFHSLNWDVDYIKPEIVLMYDLMQHHQYHHYSNYKRV
                     IDALSYYQFFILKFVVGEQRIKDAIQRTINN"
     gene            complement(888..2270)
                     /locus_tag="O2701_001590"
     CDS             complement(888..2270)
                     /locus_tag="O2701_001590"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCZ4293481.1"
                     /translation="MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDN
                     DIKTTDLLIRLYEKVINHLDYHDKKREIIQRYDDIVKELQMDESILRTIDVTLEHYIN
                     QLKNEPKRLLDALLSCGEDFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQIL
                     DSAYSEIKKLRDYDEKLIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENF
                     TDVSGIYKANPNIIKDPELIEEITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKN
                     TNRPQDKGTFIVHDREINAKNVISGISCDKDFTVINIKKYLMNRQVGFTRKILGVLED
                     NNISFDHMPSGIDTISIVMRSKQIQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGE
                     GMNKVIGTANKITHALAESKINLKMINQGASEISMMFGIHVDDAEKAVLSTYEFCYHG
                     VALKNLCDKN"
     gene            2461..3741
                     /locus_tag="O2701_001591"
     CDS             2461..3741
                     /locus_tag="O2701_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HCZ4293482.1"
                     /translation="MKKLNIALLGLGTVGSGVVKIIEENRQQIQDTLNKDIVIKHILV
                     RDKSKKRPLNISQYHLTEDVNEILNDDSLDIIVEVMGGIEPTVDWLRTALKNKKHVIT
                     ANKDLLAVHLKLLEDLAEENGVALKFEASVAGGIPIVNAINNGLNANNISKFMGILNG
                     TSNFILSKMTKEQTTFEEALDEAKRLGFAEADPTDDVEGVDAARKVVITSYLSFNQVI
                     KLNDVKRRGISGVTLTDINVADQLGYKIKLIGKGIYENGKVNASVEPTLIDKKHQLAA
                     VEDEYNAIYVIGDAVGDTMFYGKGAGSLATGSAVVSDLLNVALFFESDLHTLPPHFEL
                     KTDKTREMMDSDAEINIKEKSNFFVVVNHVKGSIENFENELKAILPFHRSLRVANYDN
                     QSYAAVIVGLESSPEELITKHGYEVDKVYPVEGV"
     gene            3747..4808
                     /locus_tag="O2701_001592"
     CDS             3747..4808
                     /locus_tag="O2701_001592"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCZ4293483.1"
                     /translation="MRRWQGLVEEFKAHLPVNENTPKLTLNEGNTPLIHCENMSKILG
                     IDLYVKYEGANPTGSFKDRGMVMAVTKAKEQGKKIVICASTGNTSASAAAYAARAGLK
                     AIVVIPEGKIALGKLSQAVMYGAEIVSIEGNFDEALEIVKEIAKSGEIELVNSVNPFR
                     IEGQKTGSFEIVQQLDGEAPDILAIPVGNAGNITAYWKGFKEYHEAKGSQLPKMFGFQ
                     AEGASPIVQNKVIKNPETIATAIRIGNPASWDKATNALKESNGLIDSVTDDEILEAYQ
                     LMTTKEGVFSEPASNASIAGLIKLHRQGKLPQGKKVVAILTGNGLKDPDTAISLLDNP
                     IKPLPNDKDSIIDYIKGAL"
     gene            4810..5724
                     /locus_tag="O2701_001593"
     CDS             4810..5724
                     /locus_tag="O2701_001593"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCZ4293484.1"
                     /translation="MSNVLELTIPASTANLGVGFDSIGMALDKFLHLSVKETSGTKWE
                     YIFHDDASKQLPTDETNFIYHVAQQVASKYSVDLPNLCIEMRSDIPLARGLGSSASAL
                     VGAIYIANYFGDIQLSKHEVLQLATEIEGHPDNVAPTIYGGLIAGYYNDVSKETSVAH
                     IDIPDVDVIVTIPTYELKTEASRRALPQKLTHSEAVKSSAISNTMICALAQHNYELAG
                     KLMQQDGFHEPYRQHLIAEFDEVKTIASQHNAYATVISGAGPTILIFSRKENSGELVR
                     SLNSQVVSCHSELVDINISGVKERIVYQ"
     gene            5782..6585
                     /locus_tag="O2701_001594"
     CDS             5782..6585
                     /locus_tag="O2701_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCZ4293485.1"
                     /translation="MTNYKVVVLDMDDTLLNSDNVISEETANYLTAIQDEGYYVVLAS
                     GRPTEGMIPTARDLKLPEHHSYIISYNGSKTINMTNEEVEVSKSIGKQDFDEIVDYCR
                     DRGFFVLTYHDGQIIYDSEHEYMNIEAELTGLPMKRVDDIKAYIQGDVPKVMGVDYVA
                     NITEARIDLNGVFNDNVDATTSKPFFLEFMAKDVSKGNAIKALCHKLGYSVDQVIAFG
                     DSMNDKSMFEVAGLAIAMGNASDELKQYANEVTLDHNENGIPHALKKLL"
     gene            complement(6878..7192)
                     /locus_tag="O2701_001595"
     CDS             complement(6878..7192)
                     /locus_tag="O2701_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293486.1"
                     /translation="MIGQTNLFDDVLKQDERFVIVVQALEEKNGQLLKRTLREYPGLN
                     HKQMNDLFMHLKELFSEESFAENQSAFSITVYTNLDYTADQIYAHVKRFRGKHDWTQT
                     AK"
     gene            complement(7167..7271)
                     /locus_tag="O2701_001596"
     CDS             complement(7167..7271)
                     /locus_tag="O2701_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293487.1"
                     /translation="MLPFFILDILHIVYNIDNNNFSGVKFNDWTNKFI"
     gene            complement(7410..8864)
                     /locus_tag="O2701_001597"
     CDS             complement(7410..8864)
                     /locus_tag="O2701_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCZ4293488.1"
                     /translation="MEDNKMNRSLNSRHISMIAIGGAIGTGLFVATGNIISQAGPGGA
                     ILAYLVIGVMLYFLMSSIGELATFYPVSGSFSSYSTRFIDSSLGFTMGWLYWALWSLV
                     TSVDVIVASNVLYFWDTFKFFHPITWSLIFITILLLLNIFSVKSFGETEFWLSLIKVL
                     TIIVFVIFGFLMIFGILGGHTYGFENYTKGQAPFVGGISGFLGVLLVAGFSVGGTEVV
                     AVTAGESDDPKKSMPKAIKQVFWRILLFYVLSIAVIGAIIPYTDPSLLRASSSISQSP
                     FTIVFDRVGIAFAASVINAVILTSLLSAANSGVYTTGRMLYSLSSDKKAPQFLSKLNK
                     TTKLPLRALLTTYAVVVIVIIYANFNSNAVFNLLEIIGSMIIVVWGSSIWSQIRLRQA
                     IKKQGQDPNKVLPYKAPFYPLGPIIVITTLLFLLFGGSVEYILKDQWLNAFKNFLPLI
                     ILALIYFIHKIIHKTKFVKLETINLKPHDYDNQK"
     gene            9068..10585
                     /locus_tag="O2701_001598"
     CDS             9068..10585
                     /locus_tag="O2701_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HCZ4293489.1"
                     /translation="MSQQDKKLTGVFGHPVSDRENSMTAGPRGPLLMQDIYFLEQMSQ
                     FDREVIPERRMHAKGSGAFGTFTVTKDITKYTNAKIFSEIGKQTEMFARFSTVAGERG
                     AADAERDIRGFALKFYTEEGNWDLVGNNTPVFFFRDPKLFVSLNRAVKRDPRTNMRDA
                     QNNWDFWTGLPEALHQVTILMSDRGIPKDLRHMHGFGSHTYSMYNDSGERVWVKFHFR
                     TQQGIENLTDEEAAEIIATDRDSSQRDLFEAIEKGDYPKWTMYIQVMTEEQAKNHKDN
                     PFDLTKVWYHDEYPLIEVGEFELNRNPDNYFMDVEQAAFAPTNIIPGLDFSPDKMLQG
                     RLFSYGDAQRYRLGVNHWQIPVNQPKGVGIENICPFSRDGQMRVVDNNQGGGTHYYPN
                     NHGKFDSQPEYKKPPFPTDGYGYEYNQRQDDDNYFEQPGKLFRLQSEDAKERIFTNTA
                     NAMEGVTDDVKRRHIRHCYKADPEYGKGVAKALGIDINSIDLETENDETYENFEK"
     gene            10676..10825
                     /gene="rpmG"
                     /locus_tag="O2701_001599"
     CDS             10676..10825
                     /gene="rpmG"
                     /locus_tag="O2701_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCZ4293490.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERIEMKKYCPRLNKYTL
                     HRETK"
     gene            11279..11548
                     /gene="rpsN"
                     /locus_tag="O2701_001600"
     CDS             11279..11548
                     /gene="rpsN"
                     /locus_tag="O2701_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCZ4293491.1"
                     /translation="MAKKSKIAKERKREELVNKYYELRKELKAKGDYEALRKLPRDSS
                     PTRLTRRCKVTGRPRGVLRKFEMSRIAFREHAHKGQIPGVKKSSW"
     gene            11705..12682
                     /gene="guaC"
                     /locus_tag="O2701_001601"
     CDS             11705..12682
                     /gene="guaC"
                     /locus_tag="O2701_001601"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCZ4293492.1"
                     /translation="MKIFDYEDIQLIPNKCIVESRSECDTTIQFGPKKFKLPVVPANM
                     QTVMNEKLAKWFAENDYFYIMHRFDEEARIPFIKHMQNSGLFASISVGVKKAEFDFIE
                     KLAQEKLIPEYITIDIAHGHSDSVINMIKHIKTHIPDSFVIAGNVGTPEGVRELENAG
                     ADATKVGIGPGRVCITKIKTGFGTGGWQLAALNICSKAARKPLIADGGIRTHGDIAKS
                     IRFGASMVMIGSLFAAHEESPGETVELDGKQYKEYFGSASEFQKGEHKNVEGKKMFVE
                     HKGSLMDTLKEMQQDLQSSISYAGGKDLKSLRTVDYVIVRNSIFNGDRD"
     gene            12704..13720
                     /locus_tag="O2701_001602"
     CDS             12704..13720
                     /locus_tag="O2701_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001789887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HCZ4293493.1"
                     /translation="MKKFIVTVVAFLSIIIIAPITEFKPFIHLQNEVRQYIDIHINKE
                     TISAENKLDTPKKQQFAFNNIQMNMSKSDVEKTLNKPKRVTFNEYGTKWYTYYDDDYN
                     NFIMISYMKDKVNALYTNQNIITSKSKIKYNTPKSVVRQRLGEPETEIVKGRVRYEQN
                     NKEYDVFHKNHIYTTVFYDKHRRNNVTAVLQVSDAMENRLKEQYGAPSKSLADSFELQ
                     NFDLVNAERKQHQLSTLKYSKQNSETARKHSKDMANNHYFDHTNLKGQSPFDRLKKDG
                     ITFNSAGENLAYGQVSSIYAHQGLMNSIGHRKNILNDTFKILGVGVDFNDEKQPFWTE
                     NYTG"
     gene            complement(14100..14723)
                     /gene="lexA"
                     /locus_tag="O2701_001603"
     CDS             complement(14100..14723)
                     /gene="lexA"
                     /locus_tag="O2701_001603"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HCZ4293494.1"
                     /translation="MRELTKRQSEIYNYIKQVVQTKGYPPSVREIGEAVGLASSSTVH
                     GHLSRLEEKGYIRRDPTKPRAIEIVSDQTNDNINMEETIHVPVIGKVTAGVPITAVEN
                     IEEYFPLPEHLTSTHNSDIFILNVVGDSMIEAGILDGDKVIVRSQTIAENGDIIVAMT
                     EEDEATVKRFYKEKNRYRLQPENSTMEPIYLDNVAVIGKVIGLYREM"
     gene            14866..15099
                     /locus_tag="O2701_001604"
     CDS             14866..15099
                     /locus_tag="O2701_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293495.1"
                     /translation="MFYNKYKNVSTYIIIFLVSSAAFAIFLLSANISAHSEQVYEMTD
                     HQIKNNTINKAYEHKDPTNNSEQRDGKVFALIN"
     gene            15235..15474
                     /locus_tag="O2701_001605"
     CDS             15235..15474
                     /locus_tag="O2701_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HCZ4293496.1"
                     /translation="MSNSDLNIERINELAKKKKEVGLTQEEAKEQTALRKAYLESFRK
                     GFKQQIENTKVIDPEGNDVTPEKIKEIQQKRDNKN"
     gene            15595..17583
                     /gene="tkt"
                     /locus_tag="O2701_001606"
     CDS             15595..17583
                     /gene="tkt"
                     /locus_tag="O2701_001606"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCZ4293497.1"
                     /translation="MFNEKDQLAVDTLRALSIDTIEKANSGHPGLPMGAAPMAYTLWT
                     RHLNFNPQSKDYFNRDRFVLSAGHGSALLYSLLHVSGSLELEELKQFRQWGSKTPGHP
                     EYRHTDGVEVTTGPLGQGFAMSVGLALAEDHLAGKFNKEGYNVVDHYTYVLASDGDLM
                     EGISHEAASFAGHNKLSKLVVLYDSNDISLDGELNKAFSENTKARFEAYGWNYLLVKD
                     GNDLEEIDKAITTAKSQEGPTIIEVKTTIGFGSPNKAGTNGVHGAPLGEVERKLTFEN
                     YGLDPEKRFNVSEEVYEIFQNTMLKRANEDESQWNSLLEKYAETYPELAEEFKLAISG
                     KLPKNYKDELPRFELGHNGASRADSGTVIQAISKTVPSFFGGSADLAGSNKSNVNDAT
                     DYSSETPEGKNVWFGVREFAMGAAVNGMAAHGGLHPYGATFFVFSDYLKPALRLSSIM
                     GLNATFIFTHDSIAVGEDGPTHEPIEQLAGLRAIPNMNVIRPADGNETRVAWEVALES
                     ESTPTSLVLTRQNLPVLDVPEDVVEEGVRKGAYTVYGSEETPEFLLLASGSEVSLAVE
                     AAKDLEKQGKSVRVVSMPNWNAFEQQSEEYKESVIPSSVTKRVAIEMASPLGWHKYVG
                     TAGKVIAIDGFGASAPGDLVVEKYGFTKENILNQVMSL"
     gene            17861..18103
                     /locus_tag="O2701_001607"
     CDS             17861..18103
                     /locus_tag="O2701_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HCZ4293498.1"
                     /translation="MATWLAIIFIVAALILGLIGGFLLARKYMMDYLKKNPPINEEML
                     RMMMMQMGQKPSQKKINQMMTMMNKNMDQNMKSAKK"
     gene            18090..18188
                     /locus_tag="O2701_001608"
     CDS             18090..18188
                     /locus_tag="O2701_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293499.1"
                     /translation="MRKSKFAIDRGYFPDMEMASFYNQINKNKYVY"
     gene            18282..18749
                     /locus_tag="O2701_001609"
     CDS             18282..18749
                     /locus_tag="O2701_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdC family protein"
                     /protein_id="HCZ4293500.1"
                     /translation="MLYLIFSIIVALFMGTIVIVIRMKAQNYPVNEKKIVLPPFFMAT
                     GALMYVVPYFRLTGSEMLEAFIIGLLFSTVLIWTSRFEVKGTEIYMKRSKAFPVILIS
                     LLIIRTVMKIFISNEIDPGELGGMFFLLAFCMIVPWRAAMLYKYKKLKKTLIN"
     gene            18873..19994
                     /locus_tag="O2701_001610"
     CDS             18873..19994
                     /locus_tag="O2701_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCZ4293501.1"
                     /translation="MKIIHTADWHLGKILNGKQLLEDQAYILDMFVEKMKEEEPDIIV
                     IAGDLYDTTYPSKDAIMLLEQAIGKLNLELRIPIIIISGNHDGKERLNYGASWFEHNQ
                     LFIRTDFTSINSPIEINGVNFYTLPYATVSEMKHYFEDDTIETHQQGITRCIETIAPE
                     IDEDAVNILISHLTVQGGKTSDSERPLTIGTVESVQKGVFDIFDYVMLGHLHHPFSIE
                     DDKIKYSGSLLQYSFSEAGQAKGYRRVTINDGIINDVFIPLKPLRQLEIISGEYNDVI
                     NEKVHVKNKDNYLHFKLKNMSHITDPMMSLKQIYPNTLALTNETFNYNEENNAIEISE
                     KDDMSIIEMFYKHITDKELSDIQSKKIKNILENELRKED"
     gene            19998..>20785
                     /locus_tag="O2701_001611"
     CDS             19998..>20785
                     /locus_tag="O2701_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCZ4293502.1"
                     /translation="MKPLHLKLNNFGPFLKEEIDFSKIDNNELFLISGKTGSGKTMIF
                     DAMTYALFGKASTEQREENDLRSHFADGKQPMSVTFEFQLNHRIYKVHRQGPYIKEGN
                     TTKTNAKFDVFEMVDGKYEIRESKVISGTQFIIELLGVNADQFRQLFILPQGEFKRFL
                     ISNSREKQGILRTLFDSEKFEAIREILKEEVKKEKAQIENRYQQIDLLWQEIESFDDD
                     NIKGLLEVATQQIDKLIENIPLLQARSKEILASVNESKETAIKE"
BASE COUNT         7566 a         2900 c         3723 g         6596 t
ORIGIN      
        1 atttcccaca tttttattag ttgattttag ttcatcattg ttataatcaa attataaact
       61 gacagatatt gatgttcaat gaatatgacg tgaaagattc gtgaattcaa gtttatgtcg
      121 aatttatgtt ataacggtca tttaaatgac agaattaggt cactcatagt attttgaaga
      181 ttgaattcat taattttaaa atgtataatg atatttgtga aagcgcttgc ttaggaggtg
      241 tatttgagag tgaatgaaat gaatgctaaa gaacaattag tggacaattt aatgaaaaca
      301 tcatcgcaat tatttaaatt tcacggtgaa gttgccatgc agcttttctt aaatgatgaa
      361 ttaaaattac cttctattgt tgaaatatgc gtggaacgta agcgtttaag tgatattgtg
      421 aaagttattc cgcaatcata tgcgttacta tacatagata agcaagatca agcaatagct
      481 aaagaagatt tatcactttc aaaaattgca aaagtttatg tgcaatatga tgatacaaca
      541 ataatgagta ttttcgttta tgatgtagta aacgatgaat ggatttttag attggatccg
      601 aatatacgta tacctaagag taacatatac ttccatagtt taaattggga tgtggattat
      661 attaaaccgg agatcgttct aatgtatgat ctaatgcaac accatcagta tcatcattat
      721 tccaattata aacgagtcat agatgcatta agctactatc aattttttat tttaaaattt
      781 gtagtaggtg agcaacgtat taaggatgca atccagagaa caataaataa ttaagaaaaa
      841 gcaattcata acgcagttga atacatgtgt tacgaattgc ttttatatta gtttttatca
      901 cacaagtttt ttaatgcaac cccgtgatag caaaactcat atgtagataa tacagctttt
      961 tcagcatcat ctacatgaat tccaaacatc atcgagattt ccgaagcacc ttggttaatc
     1021 atttttaagt taatttttga ttcagctaag gcatgtgtaa ttttatttgc agtaccgatg
     1081 actttattca taccttcgcc gacaatcatt aatattgcta aatcatgctc aatacttagc
     1141 tcatcaacat cacatttttg acgaatttca tttaatactt ttgtttcttt attttgaatt
     1201 tgttttgaac gcataacgat actgatagta tcaatacctg aaggcatatg atcaaatgaa
     1261 atattattat cctctaagac acctaatatc tttctagtaa agccgacttg tctattcatt
     1321 aaatactttt tgatattaat aacggtaaaa tctttatcac aacttatacc gctaatcaca
     1381 tttttcgcat ttatttctct atcatgcact ataaatgtac ccttatcttg agggcggttc
     1441 gtatttttaa tcaccacagg gatgcgatct ttataaagtg gttgtaaggc ttcatcatgg
     1501 aaaacactaa aaccagcata agataattca cgcatttctc gataagtgat ttcttcgatt
     1561 aattcaggat ctttgatgat atttggatta gctttataaa taccagacac atcggtgaaa
     1621 ttttcataaa ttgtggctct aacaccactt gatatgatgg cgccagttat atctgatccg
     1681 ccacgtggaa atgtaactat atatccttca tgagatacgc caaaaaatcc tgggataatt
     1741 agtttttcat cataatctct taatttttta atttcagagt aagcactatc taatatttgt
     1801 gcttcttgtg ggacgtcagt aacaaaaata cccgcttcct tcggtgatat atattttgtt
     1861 ggtatacctt gactattatt atataaagct atcaattgcg cattaaaatc ttcaccacaa
     1921 gaaagtaatg catctaatag tctctttggt tcatttttta attgattaat ataatgttcc
     1981 aaagtcacat ctatcgtccg taaaatactt tcatccattt gcaattcttt tacaatatca
     2041 tcataacgct gaataatttc tcttttttta tcatgataat caagatgatt aatgaccttt
     2101 tcatataatc tgattaacaa atcagttgtt ttaatatcat tatcatgtct tttacctgga
     2161 gcagaaacga taacaatctt tcgctctgga tcagaattaa caatatttaa aaccttttta
     2221 atttgagtag cattggagac ggagctacca ccgaatttgg aaactttcat agtgcatgcc
     2281 aacctttcta aaaaatcaga aaattgtatt cagttttgta agtaagaatg atattatgta
     2341 tttatatttt gaatattacg aactttacaa aaataaagaa taattctata ctatactatc
     2401 atctagtgtt tttcaataat acatttgttt ttctcaaaag cacaaacgga ggtacataaa
     2461 atgaaaaaat taaatatagc attattagga ttaggtactg tcggatctgg tgttgttaaa
     2521 atcatcgaag agaaccgaca gcaaattcaa gatacattaa ataaagatat tgtcataaag
     2581 catattcttg ttcgagataa atctaaaaag agaccgctaa atattagcca atatcattta
     2641 actgaagatg ttaatgaaat tttaaatgat gattcattag atattatcgt tgaagtcatg
     2701 ggaggaattg aaccaactgt agattggtta agaacagcac ttaaaaataa aaaacatgtt
     2761 attaccgcaa ataaagattt attagcagta catcttaaac ttttagaaga tttagcagaa
     2821 gaaaatggtg tagctttaaa gtttgaagcg agtgtagcag gtggtattcc gatcgtaaat
     2881 gccataaata atggtttgaa tgcgaataat atttcaaaat ttatgggaat tttaaatggt
     2941 acctctaatt ttattttatc taaaatgact aaagagcaaa cgacatttga ggaagcactt
     3001 gatgaagcga aaagacttgg ttttgctgaa gcggatccaa ctgatgatgt agaaggggta
     3061 gatgcagcgc gtaaagttgt cattacatca tatttatcat ttaaccaagt cattaaatta
     3121 aacgacgtta aacgaagagg aattagtggc gtaactttaa ctgatattaa tgtagccgat
     3181 caactggggt ataaaattaa attgattggt aagggaatat atgaaaatgg caaagttaat
     3241 gcatcggtag aaccaacgtt aattgataaa aagcatcaat tagcagctgt agaggatgaa
     3301 tataacgcga tttatgttat tggtgatgcc gttggtgaca cgatgtttta tggaaaagga
     3361 gcaggcagtt tagcaacagg tagtgccgtt gtcagtgatt tattgaatgt agcattattc
     3421 tttgaatcag atttacacac attgccacca cattttgaat taaagacaga taaaacacgg
     3481 gaaatgatgg attcagatgc agaaattaat attaaagaaa aatccaattt ctttgtagta
     3541 gtgaatcatg tcaaaggttc aattgaaaat tttgaaaatg agttaaaggc aatattacca
     3601 tttcaccgat cattaagagt tgcaaattac gataatcaat catatgccgc tgttatagtt
     3661 ggattggaat catcaccgga agaattaatc actaagcatg gatacgaagt tgacaaagta
     3721 tacccagtag aaggagttta attataatga gaagatggca aggattagta gaagagttta
     3781 aagcacattt accagtaaat gaaaatacac caaaattaac attgaacgag ggaaatacac
     3841 cactcattca ttgtgaaaat atgtctaaaa tactaggcat agatttatat gtgaagtatg
     3901 aaggtgccaa tccgacaggt tcatttaaag atcgcggtat ggtaatggct gtgacaaaag
     3961 caaaagagca aggtaagaaa attgtaatat gcgcttcgac tggaaataca tcagcgtctg
     4021 cagcagcata tgcagcgaga gcaggtttaa aagctatcgt cgtaatacca gaaggtaaaa
     4081 ttgcattagg taaattgtcg caagcagtaa tgtatggtgc agaaatcgtt tctattgaag
     4141 gaaactttga tgaagcttta gaaattgtaa aagaaattgc aaaaagtggc gaaatcgagc
     4201 ttgtaaactc tgtcaatcca tttagaatcg aaggacaaaa gacaggctca tttgaaattg
     4261 tacaacaatt agacggtgaa gcacctgata ttttagcgat tcctgtaggt aatgcaggta
     4321 atattactgc atattggaaa ggctttaaag aatatcatga agctaaagga tcacaattgc
     4381 cgaaaatgtt tggcttccaa gctgaaggcg catcaccaat tgttcaaaat aaagtcatta
     4441 aaaatcctga aacgattgca actgctattc gaattggtaa tcctgctagt tgggataagg
     4501 cgactaatgc tcttaaagaa tcaaatggat taatagatag tgttactgat gatgaaattc
     4561 tagaagcata tcagttaatg acaactaaag aaggtgtctt tagtgaacca gcgagtaatg
     4621 cttctattgc aggtttaatt aaattgcata gacaaggtaa attacctcaa ggtaaaaaag
     4681 tagttgctat tttaactggt aatggattaa aagatcctga tactgctatt tcactactag
     4741 ataatccgat aaagccattg ccaaatgata aagatagcat tatcgattat attaaaggag
     4801 ctttataaca tgtcgaatgt tttggagtta acaattcctg catcaacagc caaccttgga
     4861 gttggctttg attctatagg tatggcttta gataaatttt tgcatctgtc tgtaaaggaa
     4921 acatcaggga caaaatggga atatattttc catgatgatg catctaagca attgcctact
     4981 gacgaaacaa actttattta tcatgtagca caacaagttg cttctaaata tagtgttgac
     5041 ttgcctaatt tatgtatcga aatgagaagt gatattccat tggcaagagg gttaggttcg
     5101 tcagcttctg ctttagtagg agctatatat atcgcaaatt attttggtga tatccaactg
     5161 tctaaacatg aggtattaca attagcgact gaaatcgaag gacatcctga taatgttgcg
     5221 ccgaccattt atggtggttt aatcgctgga tattataatg atgtctcgaa agaaacgtca
     5281 gttgcacata tcgacatacc agacgtggat gtgattgtaa cgataccaac ttatgaacta
     5341 aaaacagaag catcaagacg tgctttacca caaaaattaa cacatagtga agcggttaaa
     5401 agtagtgcaa ttagtaatac aatgatttgt gcattagcac agcacaatta tgaattagca
     5461 ggtaaactca tgcaacaaga tggctttcat gaaccgtatc gtcagcattt aattgctgaa
     5521 tttgatgaag tgaaaacaat tgctagtcaa cataatgcct atgcaactgt aattagtggt
     5581 gctggaccaa ctattttaat atttagtcgt aaagaaaata gtggggaatt ggttcgctct
     5641 ttaaatagtc aggtagtatc atgccattct gaattggtcg atattaatat cagtggtgtt
     5701 aaagaacgaa ttgtatacca atagacgctt tatattgtaa aatagtatta aatgcagaat
     5761 agagaggaga tttaatgcga tatgacaaat tataaagttg tcgttttaga tatggatgac
     5821 acattgctaa attcagataa tgtgatatca gaagaaactg caaattattt aacagcaatt
     5881 caagatgaag gttattatgt tgttctagca tctggtagac ctactgaagg tatgattcca
     5941 actgctagag atttaaaatt acctgaacat catagctata ttattagtta taacggtagt
     6001 aaaacgatta acatgactaa tgaagaagta gaagtaagta aatcgattgg taagcaagat
     6061 ttcgatgaaa ttgtagatta ttgtcgagat agaggctttt tcgttcttac atatcatgat
     6121 ggtcaaatta tttacgacag cgaacatgag tatatgaata ttgaagcaga attaacaggt
     6181 ttaccgatga aacgtgttga tgatatcaaa gcgtatattc aaggcgatgt acccaaggtc
     6241 atgggtgtag attatgtagc gaatattaca gaagctagaa ttgatttgaa tggtgtgttc
     6301 aatgataatg tagatgctac gacaagtaag ccattcttct tagaatttat ggccaaagac
     6361 gtttcaaaag gtaatgcaat taaagcgtta tgtcacaaat tgggatattc ggtggatcaa
     6421 gtcattgctt ttggtgatag tatgaatgat aaatcaatgt ttgaagtcgc aggtctagct
     6481 attgctatgg ggaatgcatc agatgaactt aagcaatatg caaatgaagt tacgttggat
     6541 cataatgaaa atggtattcc acatgcgctc aaaaaattgt tataaatttt aaaataagcc
     6601 ttaacacatg atatttgaat aagatatctt gtggttaagg ctttttattt ttgtgaaaat
     6661 gacttcagtt atactatgga ggatttgaaa tacatatttt agattagtaa tgatatcaaa
     6721 cgaatagagt aaatgtatat tttttgaata aatcaagtat taactagtca cggaaggtag
     6781 ataaataaat ttgatcattc tgaataataa cgaatgcatt aaaaaatgca gtgacaatct
     6841 aacattaatt gttaaaatca ctgcaagcat cttgatttta tttagctgtt tgtgtccagt
     6901 catgcttacc tctgaaacgt tttacatgag catatatttg gtcagcagta taatctaaat
     6961 ttgtataaac tgtaatactg aacgctgatt ggttttcagc aaatgattct tcggaaaata
     7021 attcctttaa gtgcataaat aaatcattca tttgtttatg gtttaaaccg ggatattccc
     7081 ttaaagttct ctttaataat tgtccgttct tttcttctaa tgcttgaact acaataacaa
     7141 atctttcatc ttgctttagt acatcatcaa ataaatttgt ttgtccaatc attgaatttc
     7201 acccctgaaa agttattgtt atctatatta tacacgatat gcaaaatatc taaaataaag
     7261 aatggtaaca tggtaataat aaaatataaa cagaatacta gttaatacac tttatactag
     7321 aaaaagatat taaagtatat ctgctttaca ccattaaaaa gcggcaaatg ctatagataa
     7381 tctaaagcat ctgccgtatg agaatttatt tatttttgat tgtcataatc gtgtggtttt
     7441 aaattaattg tttctagctt tacaaatttt gttttgtgaa tgattttatg aataaagtaa
     7501 atcaacgcta gaatgattaa aggtaaaaag tttttaaaag catttaacca ttgatctttt
     7561 aaaatatatt caactgagcc accaaatagc aagaataata gtgtagtgat gacaatgatt
     7621 ggtcctaatg gataaaaagg tgctttatat ggtaggacct tattagggtc ttgaccttgt
     7681 tttttaatag cttgtcgcaa tcgtatttgt gaccaaatgc ttgatcccca aacaactata
     7741 atcattgaac caataatttc aagtaaatta aaaacggcat ttgaattaaa gtttgcataa
     7801 ataataacaa taacaacgac tgcataagta gttaataatg ctcttaaagg taacttagtt
     7861 gtcttgttta atttacttaa aaattggggt gcttttttgt ctgaacttaa ggaatacaac
     7921 attctgcctg ttgtataaac acctgaattt gcagcggata ataatgaagt taaaataacc
     7981 gcgttgatta ctgatgctgc aaaggctatg cctactctat cgaatacaat tgtaaatggg
     8041 ctttgactta ttgaactact tgctcttaat aatgatggat ctgtgtacgg aataattgca
     8101 ccaattactg caattgataa gacatagaat aaaagaatac gccaaaatac ttgtttaatt
     8161 gccttaggca tagacttttt agggtcatct gattcaccag cagttactgc tactacttct
     8221 gtaccaccaa ccgaaaatcc ggcgactaat aatacgccta agaaaccaga gataccacca
     8281 acaaacggtg cttggccttt tgtatagttt tcaaatccat atgtatgacc acctaagata
     8341 ccgaaaatca ttaaaaagcc aaaaataacg aatacgataa ttgttaacac tttaatcaat
     8401 gataaccaaa actcagtttc tccaaatgat tttacagaaa aaatgtttaa taatagtaaa
     8461 attgtaataa agattaagct ccaagtaatg gggtggaaaa atttaaatgt gtcccagaaa
     8521 taaagcacat ttgacgctac tatgacatca acacttgtaa ctaatgacca caatgcccaa
     8581 tacaaccatc ccatggtaaa gccaagagat gagtcaataa agcgtgttga ataagagctg
     8641 aatgaacctg atactggata aaatgttgcc aactctccaa ttgatgacat taagaaatat
     8701 agcatgacac caataacaag ataagcgagt atagcgcctc caggaccagc ttgagaaatg
     8761 atattaccag tagctacaaa tagaccagtc ccaattgcac cacctatagc aatcatggaa
     8821 atgtgtcttg agttaagact acggttcatt ttattatctt ccatatttag tctcccatct
     8881 atttaaatat acccattatt gtaagctttt taagtgtact attcaataac tatttagtac
     8941 tgtaaagcga aaaaattaaa attttctgat tttttaatca tcttgagcat gtttaattgt
     9001 aattttgatg gggttaaatt ataatatgta ttaaattata attattataa attgtggagg
     9061 gatgactatg tcacaacaag acaaaaagtt aactggtgtt tttgggcatc cagtatcaga
     9121 ccgagaaaat agtatgacag cagggcctag gggacctctt ttaatgcaag atatttactt
     9181 tttagagcaa atgtctcaat ttgatagaga agtaatacca gaacgtcgaa tgcatgccaa
     9241 aggttctggt gcatttggga catttactgt aactaaagat ataacaaaat atacgaatgc
     9301 taaaatattc tctgaaatag gtaagcaaac cgaaatgttt gcccgtttct ctactgtagc
     9361 aggagaacgt ggtgctgctg atgcggagcg tgacattcga ggatttgcgt taaagttcta
     9421 cactgaagaa gggaactggg atttagtagg gaataacaca ccagtattct tctttagaga
     9481 tccaaagtta tttgttagtt taaatcgtgc ggtgaaacga gatcctagaa caaatatgag
     9541 agatgcacaa aataactggg atttctggac gggtcttcca gaagcattgc accaagtaac
     9601 gatcttaatg tcagatagag ggattcctaa agatttacgt catatgcatg ggttcggttc
     9661 tcacacatac tctatgtata atgattctgg tgaacgtgtt tgggttaaat tccattttag
     9721 aacgcaacaa ggtattgaaa acttaactga tgaagaagct gctgaaatta tagctacaga
     9781 tcgtgattca tctcaacgcg atttattcga agccattgaa aaaggtgatt atccaaaatg
     9841 gacaatgtat attcaagtaa tgactgagga acaagctaaa aaccataaag ataatccatt
     9901 tgatttaaca aaagtatggt atcacgatga gtatcctcta attgaagttg gagagtttga
     9961 attaaataga aatccagata attactttat ggatgttgaa caagctgcgt ttgcaccaac
    10021 taatattatt ccaggattag atttttctcc agacaaaatg ctgcaagggc gtttattctc
    10081 atatggcgat gcgcaaagat atcgattagg agttaatcat tggcagattc ctgtaaacca
    10141 acctaaaggt gttggtattg aaaatatttg tccttttagt agagatggtc aaatgcgcgt
    10201 agttgacaat aaccaaggtg gaggaacaca ttattatcca aataaccatg gtaaatttga
    10261 ttctcaacct gaatataaaa agccaccatt cccaactgat ggatacggct atgaatataa
    10321 tcaacgtcaa gatgatgata attattttga acaaccaggt aaattgttta gattacaatc
    10381 agaggacgct aaagaaagaa tttttacaaa tacagcaaat gcaatggaag gcgtaacgga
    10441 tgatgttaaa cgacgtcata ttcgtcattg ttacaaagct gacccagaat atggtaaagg
    10501 tgttgcaaaa gcattaggta ttgatataaa ttctattgat cttgaaactg aaaatgatga
    10561 aacatacgaa aactttgaaa aataaatttg atatgtagtt tctatattgc gtagttgagc
    10621 agtttatgat atcataataa atcgtaaaga ttcctaacaa gagagggtgt ttaacgtgcg
    10681 cgtaaacgta acattagcat gcacagaatg tggcgatcgt aactatatca ctactaaaaa
    10741 taaacgtaat aatcctgagc gtattgaaat gaaaaaatat tgcccaagat taaacaaata
    10801 tacgttacat cgtgaaacta agtaattctt atcattcaaa tacgacgatt tgaaaataaa
    10861 gcgggcttac ctattatatt ggggagctcg cttttttatg aaatttttgt gaagagtgat
    10921 taatggattg agtttcatcg gtagaacaat atatgattat attagttgtt actttattaa
    10981 aatttgagaa tatttataga aggaaataga ttactgattt tataaagtca ctttgttagc
    11041 gaatgcttga aagagtattt aatatagtag aatttaaaat ttcaaagcgg aatttaataa
    11101 gtacgaagta attctgggta tgttttataa atgttcgata atacacttta atcttaaata
    11161 tgatggttta gaaaatgatt taacaaagaa atgaaacttt actgttgaat tatgtgagga
    11221 ttgtgttatt atataaatcg taataattac gatttgataa aaagtgaggt aactatatat
    11281 ggctaagaaa tctaaaatag caaaagagag aaaaagagaa gagttagtaa ataaatatta
    11341 cgaattacgt aaagagttaa aagcaaaagg tgattacgaa gcgttaagaa aattaccaag
    11401 agattcatca cctacacgtt taactagaag atgtaaagta actggaagac ctagaggtgt
    11461 attacgtaaa tttgaaatgt ctcgtattgc gtttagagaa catgcgcaca aaggacaaat
    11521 tccaggtgtt aaaaaatcaa gttggtaaaa tcaaatttcg tactttagcc catttacaat
    11581 atcaataaaa caatgtacag tatatacgaa tgctataaac tgaatgtttt ctcatattaa
    11641 taaggaaaca ttcggttttt aatttgcatt taaaaataat tagtttttaa aggggctatt
    11701 taaagtgaaa atatttgatt acgaagatat tcaattaata cctaataaat gcatagttga
    11761 aagtaggtct gaatgtgata caactatcca atttggtccg aaaaaattca agctacctgt
    11821 agttcctgca aatatgcaaa cagttatgaa tgagaaatta gcgaaatggt ttgctgaaaa
    11881 tgattacttt tatatcatgc atcgttttga tgaagaagca agaatacctt ttataaaaca
    11941 tatgcaaaat tcaggcttat ttgcatctat ttcagttggt gtaaagaaag cggaatttga
    12001 ttttattgaa aagttagctc aagaaaaatt aatccccgaa tatattacaa tagatattgc
    12061 gcatggtcac tcagattcag tgataaacat gattaaacat ataaaaaccc atatacctga
    12121 tagttttgtt attgctggta atgttggtac gccagaaggt gttagagaat tagaaaatgc
    12181 tggtgctgat gctaccaaag tcggtatagg tcctggtaga gtttgtatta caaagattaa
    12241 aacaggtttt ggtactggtg gttggcagtt agcggcatta aacatatgta gtaaagcagc
    12301 tcgtaaacct ttgattgccg atggtggtat aagaacgcat ggcgacattg ctaaatcaat
    12361 tagatttggt gcatcaatgg tcatgattgg ttcattattt gcggcacacg aagaatcacc
    12421 tggtgaaact gtagaacttg atggtaaaca gtataaagaa tattttggta gtgcatctga
    12481 atttcaaaaa ggcgaacata aaaatgtaga aggtaaaaaa atgtttgtag aacataaggg
    12541 ttcattaatg gataccttaa aagaaatgca acaagattta caaagctcaa tttcatatgc
    12601 cggtggaaaa gacttgaaat cattacgtac tgtagattat gttattgtta gaaactctat
    12661 tttcaacggt gatagagatt aatatttata gtaggtgatg taaattaaaa aattcatagt
    12721 aactgttgtt gcgtttttat caattattat cattgcgcca ataacagaat ttaaaccatt
    12781 cattcattta caaaatgaag taagacaata tattgacatt cacatcaata aagaaacaat
    12841 ttctgcggaa aataaattgg atacaccgaa gaaacaacaa tttgccttta ataatataca
    12901 aatgaacatg tcgaaatcag atgttgagaa aacattaaat aaaccaaaaa gagtgacatt
    12961 taatgaatat ggtacgaagt ggtatacgta ttatgatgac gattacaata attttataat
    13021 gataagttac atgaaagata aagttaatgc gttatataca aatcaaaata taatcacttc
    13081 aaaatcaaaa attaaatata atacacctaa atcggttgta aggcaaagat taggcgaacc
    13141 agaaacagag attgttaaag gtagagtgcg ttacgaacaa aataataaag aatatgatgt
    13201 tttccataaa aatcacattt atacgacggt attttatgat aagcatcgac gtaataatgt
    13261 aacagctgtt ttacaagtaa gtgatgctat ggaaaataga ttaaaagaac aatatggagc
    13321 accatcgaaa tcgcttgcag atagttttga actacaaaat tttgatttag ttaatgctga
    13381 aagaaaacaa catcaattat ctacattgaa gtattctaaa cagaattctg aaactgcacg
    13441 taagcatagt aaagatatgg ccaacaatca ttattttgat catacaaatt taaaaggtca
    13501 atcaccattt gatcgattga aaaaagatgg tattacattt aactcagccg gagagaattt
    13561 agcatatggt caagttagta gtatctatgc acatcaagga ttaatgaatt ctattggtca
    13621 cagaaaaaat attttaaatg atacgtttaa aatattaggt gttggtgttg attttaatga
    13681 tgaaaaacaa cctttttgga cagaaaatta tactggttaa tgtttttggc atgagattaa
    13741 agggtaatgt ttgtctgtaa ggaagtagaa aaacatgatg acaacagata aaccaaaaga
    13801 tgcggatata cttgaacgcg tcaaagatat acttaataaa aaagaaagaa aaaaataata
    13861 atggctcgct cctgtaaatt attacggggg cgagttttaa tttgtgtaac attttgcaga
    13921 aatctgtaat tcaaaattta tattaaagaa tgagattatc taacaattcg caatttctgt
    13981 ttgaactgaa tttagattag aataaataaa acctcttgaa aacgtaactt tttcaagagg
    14041 tccaaaattt ataaaaatga gggcttaaca attacgataa tatataacgg tttaaaatat
    14101 tacatttcgc ggtacaaacc aattactttc ccaattacag caacattgtc taggtaaatt
    14161 ggctccattg tactattttc aggttgtaat cgataacgat ttttttcttt atagaagcgt
    14221 ttgacagttg cttcatcttc ctcagtcata gcaacaataa tgtctccatt ttctgctatg
    14281 gtttgactgc gaacaattac tttgtctccg tctaatatac cagcctcaat catactgtcg
    14341 cctacgacgt ttaatatgaa tatgtcgcta ttgtgtgtcg atgttaagtg ttcaggtaat
    14401 ggaaaatatt cttcaatatt ttctactgcg gtaataggaa cacctgctgt gactttacca
    14461 ataactggca catgaatcgt ttcttccata ttaatattat catttgtttg atcacttaca
    14521 atttctatag cacgtggttt cgttggatct cttcttatat agcctttttc ttcaagacgt
    14581 gaaaggtgac catgaacagt tgaactggat gctaagccaa ctgcttcacc aatttcgcga
    14641 acactaggcg gataaccttt cgtttgaaca acttgtttaa tatagttata tatttcgctt
    14701 tgtcgttttg ttaattctct catatatagg cactccctaa attaatttaa atacagtata
    14761 tcattaattt gaaaccaaaa caaacatttg ttcgttaaaa tattgacaca gaacataagt
    14821 tctgataaat tactaataca aacaaatgtt ctaggagtga aaatgatgtt ttacaataaa
    14881 tataaaaacg tatcaacata tatcatcata tttttagttt caagtgcagc ctttgcaata
    14941 ttcttgttaa gtgcgaacat tagtgctcac tcggaacaag tgtacgaaat gactgaccat
    15001 caaattaaga acaatacgat aaataaagca tacgaacata aagaccctac aaacaatagc
    15061 gaacaaagag atgggaaagt gttcgcttta ataaattgat acattgtcac aacgttattt
    15121 tgcctatttt tgcgaaatag cgttttttat tacttttttg ctgatcttaa atttgttata
    15181 ttttgttaaa gtattataat gattgaataa acaaattgaa ggtaggtttt ttaattgagt
    15241 aattctgatt tgaatatcga aagaattaac gagttagcta aaaagaaaaa agaagtagga
    15301 ttaactcaag aagaagcaaa ggagcaaaca gccttaagaa aagcttatct tgagagtttt
    15361 agaaaagggt ttaaacaaca aattgaaaat actaaagtaa ttgatccaga aggtaatgat
    15421 gtaacacctg aaaaaattaa agagatacaa caaaaaagag ataataaaaa ttaaatcaca
    15481 aatctgtaaa gaattttctg acattataac ttgaaataag tattttactt atctttttat
    15541 tttaaaataa gttataatgt atttgataaa attgaagaag ggaagataca caagatgttt
    15601 aatgaaaaag atcaattagc tgttgatacg ctacgtgcac taagtatcga cacaatcgaa
    15661 aaagcgaatt ctggtcatcc aggattacct atgggagctg ccccaatggc ttacactttg
    15721 tggacacgtc atctgaattt taatccacaa tctaaagatt acttcaatag agaccgtttc
    15781 gtattatctg cagggcatgg ttcagcatta ttgtatagct tgttacatgt ttctggtagt
    15841 ttagaattag aagaattaaa gcaatttaga caatggggtt ctaaaacacc aggtcatcct
    15901 gaatacagac atacagatgg tgtagaagtt actaccggac cacttggaca aggttttgct
    15961 atgtcagtag gattagcttt agcagaagat cacctagcag ggaaatttaa taaagaagga
    16021 tataatgttg tagatcatta cacatatgta ttagcttctg acggtgattt aatggaaggt
    16081 atatcgcatg aagcagcttc atttgctgga cataataaat taagtaaatt agttgtttta
    16141 tacgattcaa atgatatttc attagatggc gaattaaaca aagctttttc tgaaaacaca
    16201 aaagctcgtt ttgaagcata tggttggaat tacttactag ttaaagatgg taatgattta
    16261 gaagaaattg ataaagcgat tactacagct aaatctcaag aaggaccaac gattattgaa
    16321 gttaaaacaa caatcggatt tggttcaccg aataaagcag gaactaatgg tgttcatggg
    16381 gcacctttag gtgaagttga aagaaaatta acattcgaaa attacggttt agatcctgaa
    16441 aaacgtttta atgtttcaga agaggtatac gaaattttcc aaaatactat gttaaaacgt
    16501 gctaatgaag atgaatctca atggaattca ttattagaaa aatatgcaga aacatatcct
    16561 gaattagcag aagaatttaa attagcgatt agtggtaaat tgcctaaaaa ttataaggat
    16621 gaattaccac gttttgaact gggtcataat ggtgcatctc gtgctgattc tggtactgtt
    16681 attcaagcaa tcagtaaaac tgtcccttca ttctttggtg gatcagcaga ccttgctggt
    16741 tcaaacaaat ccaatgtaaa tgatgcaact gattatagtt ctgaaacacc tgaaggtaaa
    16801 aatgtgtggt ttggtgtacg tgaatttgct atgggtgctg ctgtaaatgg tatggctgca
    16861 catggaggtt tacatccata tggtgcaaca ttcttcgtat ttagtgatta tttaaaacca
    16921 gcgttacgtt tatcatcaat tatgggatta aatgcaacgt tcatcttcac acatgattca
    16981 attgcagtag gtgaagatgg tcctactcat gaaccaattg agcaattagc tggattaaga
    17041 gccattccaa atatgaatgt tatccgtcct gctgatggta atgaaacaag agtagcatgg
    17101 gaagttgcct tagaatctga atctacacct acttcattag tattgacacg tcaaaactta
    17161 ccggtattag atgtaccaga agatgtagtt gaagaaggcg ttcgaaaagg tgcctataca
    17221 gtttatggct ctgaagagac accagaattc ctattattag cttcaggttc agaagttagt
    17281 cttgcagttg aagctgctaa agatcttgaa aaacaaggta aatcagtacg tgttgtttca
    17341 atgcctaact ggaatgcatt tgaacaacaa tctgaagaat ataaagaatc agttattcca
    17401 tcaagcgtaa caaaacgtgt tgcgattgaa atggcttcac cgcttggatg gcataaatat
    17461 gtaggtactg caggtaaagt tattgctatt gacggctttg gcgcaagtgc acctggcgat
    17521 ttagtagttg aaaaatatgg atttacaaaa gaaaatatct taaaccaagt tatgagctta
    17581 taagaataat ttataaagcg agtatgttta gaagtctagg atgcataatc ttaggcttct
    17641 tttaaagtgt tgaaatttag agtatagcac ttaaactaca tcataagtga taagttatga
    17701 aagtatacta tttcagatta atctttaaaa gctctgttat aacagcatga tttttgatat
    17761 tatttttagt atcgatatta aaatacttga ataaactagt tcttgaaata atgtgatgaa
    17821 tttagtaaaa ttcagtagga taaagaaagc gggtgaaaca atggcaactt ggttagcaat
    17881 tatttttata gtagctgcat taattttagg tttaattgga ggtttccttt tagctagaaa
    17941 atatatgatg gactacttga agaaaaaccc accaatcaac gaagaaatgc ttcgtatgat
    18001 gatgatgcaa atgggtcaaa aaccttctca gaagaaaatt aatcaaatga tgacgatgat
    18061 gaataaaaat atggatcaaa atatgaagag tgcgaaaaag taaattcgca attgatagag
    18121 gctattttcc agatatggaa atggcctctt tttataatca aattaataag aataaatatg
    18181 tttattaaaa ttaaagttaa caaaatgacg aatagactga gaaatgctat aattcatttt
    18241 gtatgattta cagagagttt atttaacgag aaggtgtctg cgtgctctat ttaatatttt
    18301 caatcattgt agctttattt atgggaacta tagttatagt tattcgtatg aaagctcaaa
    18361 attatccggt aaatgagaaa aaaatagttt tgccaccgtt ttttatggcg accggtgcat
    18421 tgatgtacgt cgttccatat tttaggctaa caggatcgga aatgctagaa gcctttataa
    18481 ttggtttgct tttttctaca gttctaattt ggacttctcg atttgaagtc aaaggtacag
    18541 aaatttatat gaaacgatct aaagcatttc cagttatttt gatttcatta cttatcattc
    18601 gtactgtgat gaaaatattc attagtaatg aaatagatcc tggagaatta ggcggcatgt
    18661 tctttttatt agcattctgt atgattgttc cttggagagc agcaatgcta tataaataca
    18721 aaaaactaaa gaaaacatta atcaattaat tacttttaaa accacttgtg atcgacttct
    18781 aaatcagtca atgagtggtt ttaattttac ttgaaaaggt gaaatgatta tattaaaata
    18841 aaagcgaaca aatgttctat atggaggcag taatgaaaat tatacataca gcagactggc
    18901 acttagggaa aatattaaat ggcaaacagc ttttagaaga tcaagcgtat attttagata
    18961 tgttcgtaga aaaaatgaaa gaagaagaac ctgatatcat tgtgatagct ggagatttat
    19021 atgacacaac atatccaagt aaagatgcaa tcatgttatt agaacaagcg attggaaagt
    19081 taaatttaga actgcgtata ccaataatta ttattagtgg aaatcacgat ggtaaagaga
    19141 ggttaaacta tggggcgagt tggtttgaac ataatcagtt atttataaga acagatttta
    19201 catcgattaa ttcaccaata gagataaatg gggttaattt ttatacactc ccttatgcta
    19261 ctgtgagcga aatgaaacac tactttgaag atgacaccat tgaaacacat caacagggaa
    19321 ttacgcgctg tattgaaaca atagcaccgg aaattgatga agatgccgtc aatattttaa
    19381 ttagtcatct gactgttcaa ggtggaaaga catctgattc tgaaagacca ttaactattg
    19441 gaacggttga atcagttcag aaaggtgttt ttgatatatt tgattatgtc atgctaggtc
    19501 acttgcatca tccatttagt atagaagacg acaaaattaa atatagtggc tccttattgc
    19561 agtattcatt ttcggaagcg ggtcaagcta aagggtatag acgtgtaaca attaatgatg
    19621 gcattattaa cgatgtattt attcctctta agccacttag acaattggaa attatctcag
    19681 gcgaatataa tgatgttatt aatgaaaaag ttcatgtgaa aaataaagat aattatttac
    19741 attttaaact taaaaatatg tctcatatta ctgatccaat gatgagttta aaacaaattt
    19801 atcctaatac tttagcgctg acgaatgaaa cttttaatta caatgaagaa aataatgcta
    19861 tagaaataag tgaaaaagat gacatgtcaa ttatcgaaat gttttataaa catataactg
    19921 ataaagaatt atcggatatc caatctaaaa agataaaaaa tattttagaa aacgaattga
    19981 gaaaggagga ttaatgaatg aaaccattac atttaaagtt gaataatttc ggcccctttt
    20041 taaaagaaga aattgatttt tctaaaattg ataataatga attgttttta ataagtggta
    20101 agactggatc gggtaaaaca atgatttttg atgcaatgac ttatgccttg tttggtaaag
    20161 catcaactga acaaagagaa gaaaatgatt tgagaagtca tttcgctgat ggtaaacagc
    20221 cgatgtcagt aacatttgaa tttcaattaa atcatcgaat ttataaagtg catagacaag
    20281 gcccttatat caaagaaggt aatacaacaa aaacgaacgc taaatttgat gtatttgaga
    20341 tggtggatgg caagtatgaa attagagaaa gtaaagtaat ttcaggtacc caattcatta
    20401 ttgaattatt aggagtaaat gcagatcaat tccgacaatt gtttattttg cctcaaggtg
    20461 aattcaaacg ctttttaata tcaaacagtc gtgaaaagca agggatatta agaacactgt
    20521 ttgacagtga aaaatttgaa gctatacgag aaatattaaa agaagaagta aaaaaagaaa
    20581 aagctcaaat cgagaataga tatcaacaaa ttgacctttt atggcaagaa attgaatcat
    20641 ttgatgatga caatataaaa ggcttattag aggttgccac tcaacagata gacaaattga
    20701 ttgaaaatat accactttta caagctaggt cgaaagaaat actagcatct gtaaatgaaa
    20761 gtaaagaaac tgctattaaa gaatt
//
LOCUS       DALAGD010000055        20466 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.055, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000055 DALAGD010000000
VERSION     DALAGD010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 20466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20466
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.055"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..387
                     /gene="lepB"
                     /locus_tag="O2701_001612"
     CDS             <1..387
                     /gene="lepB"
                     /locus_tag="O2701_001612"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCZ4293503.1"
                     /translation="VVVFHANKNDDYVKRVIGVPGDKVEYKNDTLYVNGKKQDEPYLN
                     YNLKHKQGDYITGTFQVKDLPNANPKSNVIPKGKYLVLGDNREVSKDSRAFGLIDEDQ
                     IVGKVSFRFWPFSEFKHNFNPENTKN"
     gene            547..4023
                     /gene="addB"
                     /locus_tag="O2701_001613"
     CDS             547..4023
                     /gene="addB"
                     /locus_tag="O2701_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HCZ4293504.1"
                     /translation="MTLHAYLGRAGTGKSTKMLTEIKQKMKADPLGDPIILIAPTQST
                     FQLEQAFVNDPELNGSLRTEVLHFERLSHRIFQEVGSYSEQKLSKAATEMMIYNIVQE
                     QQKYLKLYQSQAKYYGFSEKLTEQIQDFKKYAVTPEHLEHFIADKNMQTRTKNKLEDI
                     ALIYREFEQRIQNEFITGEDSLQYFIDCMPKSEWLKRADIYIDGFHNFSTIEYLIIKG
                     LIKYAKSVTIILTTDGNHDQFSLFRKPSEVLRHIEEIANELNISIERQYFNQLYRFNN
                     QDLKHLEQEFDALQINRVACQGHINILESATMREEINEIARRIIVDIRDKQLRYQDIA
                     ILYRDESYAYLFDSILPLYNIPYNIDTKRSMTHHPVMEMIRSLIEVIQSNWQVNPMLR
                     LLKTDVLTASYLKSAYLVDLLENFVLERGIYGKRWLDDELFNVEHFSKMGRKAHKLTE
                     DERNTFEQVVKLKKDVIDKILHFEKQMSQAETVKDFATAFYESMEYFELPNQLMTERD
                     ELDLNGNHEKAEEIDQIWNGLIQILDDLVLVFGDEPMSMERFLEVFDIGLEQLEFVMI
                     PQTLDQVSIGTMDLAKVDNKQHVYLVGMNDGTMPQPVTASSLITDEEKKYFEQQANVE
                     LSPTSDILQMDEAFVCYVAMTRAKGDVTFSYSLMGSSGDDKEISPFLNQIQSLFNQLE
                     ITNIPQYHEVNPLSLMQHAKQTKITLFEALRAWLYDEIVADSWLDAYQVIRDSDHLNQ
                     GLDYLMSALTFDNETVKLGETLSKDLYGKEINASVSRFEGYQQCPFKHYASHGLKLNE
                     RTKYELQNFDLGDIFHSVLKYISERINGDFKQLDLKKIRQLTNEALEEILPKVQFNLL
                     NSSAYYRYLSRRIGAIVETTLSALKYQGTYSKFMPKHFETSFRRKPRTNDELIAQTLT
                     TTQGIPINIRGQIDRIDTYTKNDTSFVNIIDYKSSEGSATLDLTKVYYGMQMQMMTYM
                     DIVLQNKQRLGLTDIVKPGGLLYFHVHEPRIKFKSWSDIDEDKLEQDLIKKFKLSGLV
                     NADQTVIDALDIRLEPKFTSDIVPVGLNKDGSLSKRGSQVADEATIYKFIQHNKENFI
                     ETASNIMDGHTEVAPLKYKQKLPCAFCSYQSVCHVDGMIDSKRYRTVDETINPIEAIQ
                     NININDEFGGEQ"
     gene            4024..7677
                     /gene="addA"
                     /locus_tag="O2701_001614"
     CDS             4024..7677
                     /gene="addA"
                     /locus_tag="O2701_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HCZ4293505.1"
                     /translation="MTIPEKPQGVIWTDAQWQSIYATGQDVLVAAAAGSGKTAVLVER
                     IIQKILRDGIDVDRLLVVTFTNLSAREMKHRVDQRIQEASIADPANAHLKNQRIKIHQ
                     AQISTLHSFCLKLIQQHYDVLNIDPNFRTSSEAENILLLEQTIDEVIEQHYDILDPAF
                     IELTEQLSSDRSDDQFRMIIKQLYFFSVANPNPKNWLDQLVTPYEEEAQQAQLIQLLT
                     DLSKVFITAAYDALNKAYDLFSMMDSVDKHLAVIEDERRLMGRVLEGGFIDIPYLTGH
                     EFGARLPNVTAKIKEANEMMVDALEDAKLQYKKYKSLIDKVKSDYFSREADDLKADMQ
                     QLAPRVKYLARIVKDVMSEFNRKKRSKNILDFSDYEHFALQILTNEDGSPSEIAESYR
                     QHFQEILVDEYQDTNRVQEKILSCIKTGDEHNGNLFMVGDVKQSIYKFRQADPSLFIE
                     KYQRFTIDGDGTGRRIDLSQNFRSRKEVLSTTNYIFKHMMDEQVGEVKYDEAAQLYYG
                     APYDESDHPVNLKVLVEADQEHSDLTGSEQEAHFIVEQVKDILEHQKVYDMKTGSYRS
                     ATYKDIVILERSFGQARNLQQAFKNEDIPFHVNSREGYFEQTEVRLVLSFLRAIDNPL
                     QDIYLVGLMRSVIYQFKEDELAQIRILSPNDDYFYQSIVNYINDEAADAILVDKLKMF
                     LSDIQSYQQYSKDHPVYQLIDKFYNDHYVIQYFSGLIGGRGRRANLYGLFNKAIEFEN
                     SSFRGLYQFIRFIDELIERGKDFGEENVVGPNDNVVRMMTIHSSKGLEFPFVIYSGLS
                     KDFNKRDLKQPVILNQQFGLGMDYFDVDKEMAFPSLASVAYRAVAEKELVSEEMRLVY
                     VALTRAKEQLYLIGRVKNDKSLLELEQLSISGEHIAVNERLTSPNPFHLIYSILSKHQ
                     SASIPDDLKFEKDIAQIEDSSRPNVNISIVYFEDVSTETILDNDEYRSVNQLETMQNG
                     NEDVKAQIKHQLDYRYPYVNDTKKPSKQSVSELKRQYETEESGTSYERVRQYRIGFST
                     YERPKFLSEQGKRKANEIGTLMHTVMQHLPFKKERISEVELHQYIDGLIDKHIIEADA
                     KKDIRMDEIMTFINSELYSIIAEAEQVYRELPFVVNQALVDQLPQGDEDVSIIQGMID
                     LIFVKDGVHYFVDYKTDAFNRRRGMTDEEIGTQLKNKYKIQMKYYQNTLQTILNKEVK
                     GYLYFFKFGTLQL"
     gene            7843..8745
                     /locus_tag="O2701_001615"
     CDS             7843..8745
                     /locus_tag="O2701_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCZ4293506.1"
                     /translation="MKFLSFKYNDKTSYGVKVKREDAVWDLTQVFADFAEGDFHPKTL
                     LAGLQQNHTLDFQEQVRKAVVAAEDSGKAEDYKISFNDIEFLPPVTPPNNVIAFGRNY
                     KDHANELNHEVEKLYVFTKAASSLTGDNATIPNHKDITDQLDYEGELGIVIGKSGEKI
                     PKALALDYVYGYTIINDITDRKAQSEQDQAFLSKSLTGGCPMGPYIVTKDELPLPENV
                     NIVTKVNNEIRQDGNTGEMILKIDELIEEISKYVALHPGDIIATGTPAGVGAGMQPPK
                     FLQPGDEVKVTIDNIGTLTTYIAK"
     gene            9074..9463
                     /locus_tag="O2701_001616"
     CDS             9074..9463
                     /locus_tag="O2701_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YisL family protein"
                     /protein_id="HCZ4293507.1"
                     /translation="MLHLHILSWVLAIILFIATYLNISKNQGRSPFFKPLHMILRLFM
                     LLTLISGFWILIQSFMNGGANHMLLTLKMLCGVAVVGLMEVSIAKRKRHEQSHTMFWI
                     TIALIIITMVLGVILPLGPISKLFGIG"
     gene            complement(9634..10950)
                     /locus_tag="O2701_001617"
     CDS             complement(9634..10950)
                     /locus_tag="O2701_001617"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HCZ4293508.1"
                     /translation="MPKIVVVGAVAGGATCASQIRRLDKESDIIIFEKDRDMSFANCA
                     LPYVIGEVVEDRRYALAYTPEKFYDRKQITVKTYHEVIAINDERQTVSVLNRKTNEQF
                     EESYDKLILSPGASANSLGFESDITFTLRNLEDTDAIDQFIKANQVDKVLVVGAGYVS
                     LEVLENLYERGLHPTLIHRSDKINKLMDADMNQPILDELDKREIPYRLNEEINAINGN
                     EITFKSGKVEHYDMIIEGVGTHPNSKFIESSNIKLDRKGFIPVNDKFETNVPNIYAIG
                     DIATSHYRHVDLPASVPLAWGAHRAASIVAEQIAGNDTIEFKGFLGNNIVKFFDYTFA
                     SVGVKPNELKQFDYKMVEVTQGAHANYYPGNSPLHLRVYYDTSNRQILRAAAVGKEGA
                     DKRIDVLSMAMMNQLTVDELTEFEVAYAPPYSHPKDLINMIGYKAK"
     gene            complement(11002..11826)
                     /locus_tag="O2701_001618"
     CDS             complement(11002..11826)
                     /locus_tag="O2701_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCZ4293509.1"
                     /translation="MQPHLICLDLDGTLLNDNKEISSYTKQVLNELQQRGHQIMIATG
                     RPYRASQMYYHELNLTTPIVNFNGAYVHHPKDKNFKTCHEILDLGIAQNIIQGLQQYQ
                     VSNIIAEVKDYVFINNHDPRLFEGFSMGNPRIQTGNLLVHLKESPTSILIEAEESKIP
                     EIKNMLTHFYADHIEHRRWGAPFPVIEIVKLGINKARGIEQVRQFLNIDRNNIIAFGD
                     EDNDIEMIEYARHGVAMENGLQELKDVANNITFNNNEDGIGRYLNDFFNLNIRYYC"
     gene            11940..12248
                     /locus_tag="O2701_001619"
     CDS             11940..12248
                     /locus_tag="O2701_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HCZ4293510.1"
                     /translation="MEEALKDSILGALEMVIDPELGIDIVNLGLVYKVNVDDEGVCTV
                     DMTLTSMGCPMGPQIIDQVKTVLAEIPEIQDTEVNIVWSPPWTKDMMSRYAKIALGVS
                     "
     gene            12795..14609
                     /locus_tag="O2701_001620"
     CDS             12795..14609
                     /locus_tag="O2701_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCZ4293511.1"
                     /translation="MNKTKGFTKYKKMRYIPGLDGLRAIAVLGIIIYHLNKQWLTGGF
                     LGVDTFFVISGYLITSLLLKEYDDTGIIKLKSFWIRRLKRLLPAVIVLLMVVGTATLL
                     LKSDNIIRVKHDIIAAIFYVSNWWYIAKDVNYFEQFSFMPLKHLWSLAIEEQFYIFFP
                     VILVTLLLTIKKRYKIGFIFWGVSIISLGLMMFIYSINGDHSRVYFGTDTRLQTLLLG
                     VILAFLWPPFKLKNDPPKVVKYVIDSIGSLSFIVLILLFFIINDETNWIYDGGFYLIS
                     ILTLFIIASVVHPSTWIAKIFSNPVLVFIGKRSYSLYLWHFAVISFVHSYYVDGQIPV
                     YVYFIDISLTIIFAELSYRFIETPFRKEGIKALNWRPSYIPQFIRMAIVVTLLIPFML
                     ILVGAFNKYGKDIIGEKANSFDTTIEDNYLMRIAPIDNIHIDGLVSEKKKESSDVYNN
                     IKPLLIGDSVMVDIGESFKSSVPKSRIDGKVGRQLYQTLPLVKANYSQYKKSSDQVVL
                     ELGTNGDFTVKQLDDLLNQFGKAKIYLVNTRVPRIYEANVNRLLADAAKRKSNVTLID
                     WYKRSQGHSEYFAPDGVHLEYKGVLALKDEILKALKKK"
     gene            14812..17421
                     /gene="clpB"
                     /locus_tag="O2701_001621"
     CDS             14812..17421
                     /gene="clpB"
                     /locus_tag="O2701_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCZ4293512.1"
                     /translation="MDINKMTYAVQSALQQAVELSQQHKLQNIEIEAILSAALNESES
                     LYKSILERANIEVDQLNKAYEDKLNTYASVEGDNIQYGQYISQQANQLITKAESYMKE
                     YEDEYISMEHILRSAMDIDQTTKHYINNKVEVIKEIIKKVRGGNHVTSQNPEVNYEAL
                     AKYGRDLVEEVRQGKMDPVIGRDEEIRNTIRILSRKTKNNPVLIGEPGVGKTAIVEGL
                     AQRIVKKDVPESLLDKTVFELDLSALVAGAKYRGEFEERLKAVLKEVKESDGRIILFI
                     DEIHMLVGAGKTDGAMDAGNMLKPMLARGELHCIGATTLNEYREYIEKDSALERRFQK
                     VAVSEPDVEDTISILRGLKERYEVYHGVRIQDRALVAAAELSDRYITDRFLPDKAIDL
                     VDQACATIRTEMGSNPTELDQVNRRVMQLEIEESALKNESDNASKQRLQELQEELANE
                     KEKQAALQSRVESEKEKIANLQEKRAQLDESRQALEDAQTNNNLEKAAELQYGTIPQL
                     EKELRELEDNFQDEQGEDTDRMIREVVTDEEIGDIVSQWTGIPVSKLVETEREKLLHL
                     SDILHKRVVGQDKAVDLVSDAVVRARAGIKDPNRPIGSFLFLGPTGVGKTELAKSLAA
                     SLFDSEKHMIRIDMSEYMEKHAVSRLIGAPPGYIGHDEGGQLTEAVRRNPYSVILLDE
                     VEKAHTDVFNVLLQILDEGRLTDSKGRSVDFKNTIIIMTSNIGSQVLLENVKETGEIT
                     ESTEKAVMTSLNAYFKPEILNRMDDIVLFKPLSIDDMSMIVDKILTQLNIRLLEQRIS
                     IEVSDDAKAWLGQEAYEPQYGARPLKRFVQRQIETPLARMMIKEGFPEGTTIKVNLNS
                     DNNLTFNVEKIHE"
     gene            complement(17480..18349)
                     /locus_tag="O2701_001622"
     CDS             complement(17480..18349)
                     /locus_tag="O2701_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCZ4293513.1"
                     /translation="MNLDWYYTFVILAKTLNYRLASEEINLTIPSIHKQIKNLEQHLN
                     VKLFETYKNQIILTEDGHTFLPIAQSFIEQYESGIKHIQLKKTMFQSKLNVVVSSYIA
                     TFIMPKFLKSFFNEHPFIDVSLHVKNENIEKDINNHTYDIGISRNQPKLREVHSEKVC
                     EGKIVLIAPNKENNHLLTEASLFEKYKIISDNHPEYWSSLKNNILNIYEKAQFLSIND
                     VHTSIKLIEMNQGISFLPLYITTNSDYNISVINTKILQAPISFTYIYSKKESPEILVF
                     IKSFKKYIANEQL"
     gene            18459..19604
                     /locus_tag="O2701_001623"
     CDS             18459..19604
                     /locus_tag="O2701_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCZ4293514.1"
                     /translation="MIRIQDNTIRDGMQQSNVRKSLIIKKEVLKQINKLNINSVEVGM
                     CTTIEDEFNIHQFRDILSPEKELVVLTRLNEKEIKKIVKLKIHNLVVKILLPISDLHI
                     KEKLNFSNKYYIQKIKDCLDILKKDKKGVDICFEDGTRTSREKLKEYMEIISKYQVRT
                     VTFADTVGCSTPLEYGDIFNYFVKKYSNIIFSAHCHNDLGLATANTLAAILNGAKQIE
                     TTFLRIGERAGNAPIEEIITILTKKQIESTEFTLPDVYKTSINISKILDFQISENKPI
                     IGENIFKHESGIHQDGTKKNINMYQYLVPSDLGLKNSQVVQFPISNISSKKILHNKFK
                     SIVNTEEIDENISFYKLVNKFSPEVAPEDTVDLLQIIKRRSKDGNFK"
     gene            19588..20229
                     /locus_tag="O2701_001624"
     CDS             19588..20229
                     /locus_tag="O2701_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HCZ4293515.1"
                     /translation="MEILNDSVKSFEKLYLQLQDGFPVIVPTDTNYNLCSLPNNDLCI
                     DKIFEYKKRSKDKPLSLFIDKPEDWKLYGDNQNTEIVDKLVEIFWPGPLNIILKNKTS
                     YNYMLNNSDSIAIGCVQNKTMRRFISYINSPIAITSANISGTADDILITENEAIKHMG
                     ENVRYMLRSQNKTNYKTSSTIIKVTDNKIELLREGDIKFEEIKERLGTGIIYE"
     gene            20222..>20466
                     /locus_tag="O2701_001625"
     CDS             20222..>20466
                     /locus_tag="O2701_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293516.1"
                     /translation="MNKLILGIYLYRIFSRAYFYLPFLLIYFLIQGYSIIQLEILMAS
                     YGIAAFLFSLYKEKCFKICNLKDSNKLVVSEIFKIIG"
BASE COUNT         7408 a         2799 c         3621 g         6638 t
ORIGIN      
        1 gtagttgtct tccatgcaaa caaaaatgat gactatgtta aacgtgtcat cggtgttcct
       61 ggtgataaag tagaatacaa aaatgataca ttatatgtca atggtaaaaa acaagatgaa
      121 ccatatttaa actacaattt aaaacataaa caaggtgatt acattactgg gactttccaa
      181 gttaaagatt taccgaatgc gaatcctaaa tcaaatgtca ttccaaaagg taaatattta
      241 gtgcttggag ataatcgtga agtaagtaaa gatagccgtg cgtttggcct cattgatgaa
      301 gaccaaattg ttggtaaagt ttcatttagg ttctggccat ttagtgaatt taaacataat
      361 ttcaatcctg aaaatactaa aaattaatat gaaacaaata caacatcgtt tgtcggtttt
      421 aatactgata aacgatgttt tattttgtta gtaccacaat aaaagctaag ttcgaaatga
      481 acttataata aatcaatcac aatcactttg tgttaaaata tgtgtcaaag gaagtgaggg
      541 tttgtcatga cattacatgc ttatttaggt agagcgggaa caggtaagtc tacgaaaatg
      601 ttgaccgaaa taaaacaaaa aatgaaagca gatccgcttg gagatccaat cattttaatt
      661 gcgccaactc aaagtacatt tcaattagaa caagcctttg tcaatgatcc ggaattaaat
      721 ggtagtttaa gaacagaagt gttgcatttt gaacgattaa gtcatcgtat tttccaagaa
      781 gttggtagtt atagcgaaca aaagttatct aaagctgcaa cggaaatgat gatttataac
      841 attgttcaag aacaacaaaa gtatttaaaa ctttatcaat cacaagcaaa atattatggg
      901 tttagtgaaa aattaacaga acaaattcaa gattttaaaa aatatgcagt aacgcctgaa
      961 catttagaac actttattgc tgataaaaat atgcaaactc gaactaaaaa taagttagag
     1021 gatattgctt taatataccg tgagttcgaa caacgcattc aaaacgagtt tattacgggt
     1081 gaggattcat tacaatattt tattgattgt atgccgaaat cagagtggct aaaacgtgct
     1141 gatatatata ttgatggttt tcacaacttt tcaacgattg agtatttaat aatcaaagga
     1201 ttaattaaat atgcgaagag tgtcacaatt atattgacga cagatggtaa ccacgatcaa
     1261 tttagtttat ttagaaaacc atcggaagtg ttacgacata ttgaagaaat agcaaatgaa
     1321 ctcaatattt ctattgaacg tcaatatttc aaccaattat atcgcttcaa taatcaagat
     1381 ttaaagcatc ttgaacaaga atttgatgca cttcaaatca atcgagtggc atgtcaaggt
     1441 catatcaata ttttagaatc tgcgactatg agagaggaaa taaatgaaat tgcgcgacgt
     1501 atcatcgttg atattcgtga taagcaatta cgatatcaag atattgctat tttatatcgt
     1561 gatgaatctt atgcttattt atttgattcc atattaccgc tttataatat tccttataat
     1621 attgatacaa agcgttcgat gacacatcat ccggtcatgg aaatgattcg ttcattgatt
     1681 gaagttattc aatctaattg gcaagtgaat ccaatgctac gcttattgaa gactgatgtg
     1741 ttaacggcat catatctaaa aagtgcatac ttagttgatt tacttgaaaa ttttgtactt
     1801 gaacgtggta tatacggtaa acgttggtta gatgatgagc tatttaatgt cgaacatttt
     1861 agcaaaatgg ggcgtaaagc gcataaactg accgaagatg aacgtaacac atttgaacaa
     1921 gtcgttaagt taaagaaaga tgtcattgat aaaattttac attttgaaaa gcaaatgtca
     1981 caagcggaaa ctgtaaaaga ttttgcaact gctttttatg aaagtatgga atatttcgaa
     2041 ctgccaaatc aattgatgac agagcgagat gaacttgatt taaatggtaa tcatgaaaag
     2101 gcggaggaaa ttgatcaaat atggaatggc ttaattcaaa tccttgatga cttagttcta
     2161 gtatttggag atgaaccaat gtcgatggaa cgtttcttag aagtatttga tattggttta
     2221 gaacaattag aatttgttat gattccgcaa acattggacc aagtaagtat tggtacgatg
     2281 gatttggcta aagtcgataa taagcaacat gtttacttag taggtatgaa tgatggaacg
     2341 atgccacaac cagtaactgc gtcaagcttg attacagatg aagaaaagaa atactttgaa
     2401 cagcaggcta atgtcgagtt aagtccaaca tcagatattt tacagatgga tgaagcattt
     2461 gtttgttatg ttgctatgac tagagctaag ggagatgtta cattttctta cagtctaatg
     2521 ggatcaagtg gtgatgataa ggagatcagc ccatttttaa atcaaattca atcattgttc
     2581 aaccaattgg aaattactaa cattcctcaa taccatgaag ttaacccatt gtcactaatg
     2641 caacatgcta agcaaaccaa aattacatta tttgaagcat tgcgtgcttg gttatatgat
     2701 gaaattgtgg ctgatagttg gttagatgct tatcaagtaa ttagagatag cgatcattta
     2761 aatcaaggtt tagattattt aatgtcagca ttaacgtttg acaatgaaac tgtaaaatta
     2821 ggtgaaacgt tgtctaaaga tttatatggt aaggaaatca atgccagtgt atcccgtttt
     2881 gaaggttatc aacaatgccc atttaaacac tatgcgtcac atggtctgaa actaaatgag
     2941 cgaacgaagt atgaacttca aaactttgat ttaggtgata ttttccattc tgttttaaaa
     3001 tatatatctg aacgtattaa tggcgatttt aaacaattag acctgaaaaa aataagacaa
     3061 ttaacgaatg aagcattgga agaaatttta cctaaagttc agtttaattt attaaattct
     3121 tcagcttact atcgttattt atcaagacgc attggcgcta ttgtagaaac aacactaagc
     3181 gcattaaaat atcaaggcac gtattcaaag tttatgccaa aacattttga gacaagtttt
     3241 agaaggaaac caagaacaaa tgacgaatta attgcacaaa cattaacgac aactcaaggt
     3301 attccaatta atattagagg gcaaattgac cgtatcgata cgtatacaaa gaatgataca
     3361 agttttgtta atatcattga ctataaatcc tctgaaggta gtgcgacact tgatttaacg
     3421 aaagtatatt atggtatgca aatgcaaatg atgacataca tggatatcgt tttacaaaat
     3481 aaacaacgcc ttggattaac agatattgtg aaaccaggtg gattattata cttccatgta
     3541 catgaaccta gaattaaatt taaatcatgg tctgatattg atgaagataa actagaacaa
     3601 gatttaatta aaaagtttaa gttgagtggt ttagttaatg cagaccaaac tgttattgat
     3661 gcattggata ttcgtttaga acctaaattc acttcagata ttgtaccagt tggtttgaat
     3721 aaagatggct ctttgagtaa acgaggcagc caagtggcag atgaagcaac gatttataaa
     3781 ttcatccaac ataacaaaga gaattttata gaaacagctt caaatattat ggatggacat
     3841 actgaagttg caccattaaa gtacaaacaa aaattgccat gtgctttttg tagttatcaa
     3901 tcggtatgtc atgtagatgg catgattgat agtaagcgat atcgaactgt agatgaaaca
     3961 ataaatccaa ttgaagcaat tcaaaatatt aacattaatg atgaatttgg gggtgagcaa
     4021 tagatgacaa ttccagagaa accacaaggc gtgatttgga ctgacgcgca atggcaaagt
     4081 atttacgcaa ctggacaaga tgtacttgtt gcagccgcgg caggttcagg taaaacagct
     4141 gtactagttg agcgtattat ccaaaagatt ttacgtgatg gcattgatgt cgatcgactt
     4201 ttagtcgtaa cgtttacaaa cttaagcgca cgtgaaatga agcatcgtgt agaccaacgt
     4261 attcaagagg catcgattgc tgatcctgca aatgcacact tgaaaaacca acgcatcaaa
     4321 attcatcaag cacaaatatc tacactccat agtttttgct tgaaattaat tcaacagcat
     4381 tatgatgtat taaatattga cccgaacttt agaacaagca gtgaagctga aaatatttta
     4441 ttattagaac aaacgataga tgaggtcata gaacaacatt acgatatcct tgatcctgct
     4501 tttattgaat taacagagca attgtcttca gatagaagtg atgatcagtt tcgaatgatt
     4561 attaaacaat tgtatttctt tagcgttgca aatccaaatc ctaaaaattg gttggatcaa
     4621 ttggtgacac catacgaaga agaagcacaa caagcgcaac ttattcaact actaacagac
     4681 ttatctaaag tatttatcac agctgcttat gatgctttaa ataaggcgta tgatttgttt
     4741 agtatgatgg atagcgtcga taaacattta gctgttatag aagatgaacg acgtttaatg
     4801 gggcgtgttt tagaaggtgg ctttattgat ataccttatt taactggtca cgaatttggc
     4861 gcgcgtttgc ctaatgtaac agcgaaaatt aaagaagcaa atgaaatgat ggtcgatgcc
     4921 ttagaagatg ctaaacttca gtataaaaaa tataaatcat taattgataa agtgaagagt
     4981 gattactttt caagagaagc tgatgatttg aaagctgata tgcaacaatt ggcgccacga
     5041 gtaaagtacc ttgcgcgtat tgtgaaagat gttatgtcag aattcaatcg aaaaaagcgt
     5101 agcaaaaata ttttggattt ttctgattat gaacattttg cattacaaat tttaactaat
     5161 gaggatggtt cgccttcaga aattgccgaa tcataccgtc aacacttcca agaaatattg
     5221 gtcgatgagt atcaagatac gaaccgagtt caagagaaaa tactatcttg catcaaaacg
     5281 ggtgatgaac ataatggtaa tttatttatg gttggagatg ttaagcaatc catttataaa
     5341 tttagacaag ctgatccaag tttatttatt gaaaagtatc aacgctttac tatagatgga
     5401 gatggcactg gacgtcgaat tgatttgtcg caaaacttcc gttctcgaaa agaagtactg
     5461 tcaacgacta actatatatt caaacatatg atggatgaac aagtcggtga agtaaaatat
     5521 gatgaagcgg cacagttgta ttatggtgca ccatatgatg aatcggacca tccagtaaac
     5581 ttaaaagtcc ttgttgaagc ggatcaagaa catagtgatt taactggtag tgaacaagaa
     5641 gcgcatttta tagtagaaca agttaaagat atcttagaac atcaaaaagt ttatgatatg
     5701 aaaacaggaa gctatagaag tgcgacatac aaggatatcg ttattctaga acgcagcttt
     5761 ggacaagctc gcaatttaca acaagccttt aaaaatgaag atattccatt ccatgtgaat
     5821 agtcgtgaag gttactttga acaaacagaa gtccgcttag tattatcatt tttaagagcg
     5881 atagataatc cattacaaga tatttattta gttgggttaa tgcgctccgt tatatatcag
     5941 ttcaaagaag acgaattagc tcaaattaga atattgagtc caaatgatga ctacttctat
     6001 caatcgattg taaattacat taatgacgaa gcagcagatg caattttagt tgataaatta
     6061 aaaatgtttt tatcagatat tcaaagttac caacaatata gtaaagatca tccggtgtat
     6121 cagttaattg ataaatttta taatgatcat tatgttattc aatactttag tggacttatt
     6181 ggtggacgtg gacgacgtgc aaatctttat ggtttattta ataaagctat cgagtttgag
     6241 aattcaagtt ttagaggttt atatcaattt attcgtttta tcgatgaatt gattgaaaga
     6301 ggcaaagatt ttggtgagga aaatgtagtt ggtccaaacg ataatgtcgt tagaatgatg
     6361 acaattcata gtagtaaagg tctagagttt ccatttgtca tttattctgg attgtcaaaa
     6421 gattttaata aacgtgattt gaaacaacca gttattttaa atcagcaatt tggtctcgga
     6481 atggattatt ttgatgtgga taaagaaatg gcatttccat ctttagcttc ggttgcatat
     6541 agagctgttg ccgaaaaaga acttgtgtca gaagaaatgc gattagtcta tgtagcatta
     6601 acaagagcga aagaacaact ttatttaatt ggtagagtga aaaatgataa atcattacta
     6661 gaactagagc aattgtctat ttctggtgag cacattgctg tcaatgaacg attaacttca
     6721 ccaaatccgt tccatcttat ttatagtatt ttatctaaac atcaatctgc gtcaattcca
     6781 gatgatttaa aatttgaaaa agatatagca caaattgaag atagtagtcg tccgaatgta
     6841 aatatttcaa ttgtgtactt tgaagatgtg tctacagaaa ccattttaga taatgatgaa
     6901 tatcgttcgg ttaatcaatt agaaactatg caaaatggta atgaagatgt taaagcacaa
     6961 attaaacacc aacttgatta tcgatatcca tatgtaaatg atactaaaaa gccctcaaaa
     7021 caatctgttt ctgaattgaa aagacaatat gaaacagaag aaagtggcac aagttacgaa
     7081 cgagtaaggc aatatcgtat cggtttttca acgtatgaac gacctaaatt tctaagtgaa
     7141 caaggtaaac gaaaagcgaa tgaaattggt acgttaatgc atacagtgat gcaacattta
     7201 ccattcaaaa aagaacgcat atctgaagtt gagttacatc agtatatcga tggattaatc
     7261 gataaacata ttatcgaagc agatgcgaaa aaagatatcc gtatggatga aataatgaca
     7321 tttatcaata gtgagttata ttcgattatt gctgaagcag agcaagttta tcgtgaatta
     7381 ccgtttgtag ttaaccaagc attagttgac caattgccac aaggagacga agacgtctca
     7441 attattcaag gtatgattga cttaatcttt gttaaagatg gtgtgcatta ttttgtagac
     7501 tataaaaccg atgcatttaa tcgtcgccgt gggatgacag atgaagaaat tggtacacaa
     7561 ttaaaaaata aatataagat acagatgaaa tattatcaaa atacgcttca aacgattctt
     7621 aataaagaag ttaaaggtta tttatacttc ttcaaatttg gtacattgca actatagtat
     7681 tttgattttc aaaagaataa aaaataattt cgattaagtg caaagtcctt gtagcagaat
     7741 gaacacaact cattttcaaa attgtcttac ttatttattt gttatttgat aacgaaaaaa
     7801 gttataatgt gaattaagat aaagatgagg agttgagaat gaatgaaatt cttatcattc
     7861 aagtataatg acaaaacttc atatggcgtt aaagtaaaac gcgaagatgc tgtatgggat
     7921 ttaacacaag tatttgctga ctttgcagaa ggagatttcc atcctaaaac attgttagct
     7981 ggtttacaac aaaatcatac tttagatttt caagaacaag tacgtaaagc agttgtagca
     8041 gcagaagata gcggcaaagc tgaagactat aaaatttcat ttaatgacat tgaattttta
     8101 ccaccagtaa cacctccgaa taatgtgatt gcttttggta gaaattacaa agatcatgcg
     8161 aacgaattaa atcatgaagt ggaaaaatta tatgtattta caaaagcagc gtcatcttta
     8221 acaggagata atgcaacaat tccaaatcat aaagatatta ctgatcaatt agattatgaa
     8281 ggtgaattag gtattgttat tggtaagtct ggtgaaaaga ttccaaaagc attagcttta
     8341 gattatgttt acggctatac aattattaac gatatcactg atcgcaaagc acaaagtgaa
     8401 caagatcaag catttttatc aaaaagttta actggcggtt gcccaatggg tccttatatc
     8461 gttactaaag acgaactacc attacctgaa aatgtaaata ttgttacaaa agttaacaat
     8521 gaaattagac aagatggtaa cactggcgaa atgattctta aaattgatga attaatagaa
     8581 gaaatttcaa aatatgttgc actacatccg ggagatatta ttgcaactgg tacaccagca
     8641 ggcgttggtg caggtatgca accacctaaa tttttacaac caggtgatga agttaaagtg
     8701 actattgata atattggaac gctgacaact tatatcgcta aataattatc atttaaaaag
     8761 ctaaccagtc tttatataga ttggttagtt ttttcttgct tttctaaaaa ggtgttaaag
     8821 ataaattatt tataatgtta ccattttgag atgaaagtga aatattgata ttaagaagta
     8881 gttgattatt ttacagcaga ttcacaatat tctaataagg aataaaatgt catgttcttc
     8941 ctctcaaata tagaagtgtg gtagaatata tattcgtgta taatcaaatc atgattaaat
     9001 tacaagcaag tgggtattaa tcccaagaag ctttaattta ataatataaa taattaaagt
     9061 ggggggaatc agtatgttac atttacatat attaagttgg gtattagcga ttattttatt
     9121 tatcgctaca tacttaaaca tttcaaaaaa tcaaggcaga tcaccatttt tcaaaccgtt
     9181 gcacatgatt ttacgcttat ttatgctgtt gacgttaatt tcaggatttt ggatattaat
     9241 tcagtcattt atgaatggcg gggcaaatca tatgttgctt acattgaaaa tgctgtgtgg
     9301 tgttgcagta gttggattga tggaagtgtc gattgctaaa agaaagagac atgaacaaag
     9361 tcacacaatg ttttggataa caattgcatt aattatcatc acaatggtat taggtgtcat
     9421 tctaccgtta gggcctatat caaaattatt cggtattggc taattgataa gacatatagt
     9481 atatgacata aggcatggac aattattagt ccaagacctt atgtcttttt tgattctaaa
     9541 aatgttaaac ataaaaaatg gactggatag tagtaccagt ccaacataat tctaacctag
     9601 acaattaaga ttagattgaa tcgcatgatt catttattta gctttgtaac caatcatatt
     9661 gattaaatct ttagggtggc tatatggtgg tgcataagcc acttcaaact cagttaactc
     9721 atctacagtt agctggttca tcattgccat cgatagtaca tcaatacgtt tatctgcacc
     9781 ttcttttcct actgcagctg ctcttaaaat ctgacggttt gaagtgtcat aatatactct
     9841 taagtgtaaa ggggaatttc ctgggtaata attcgcgtgt gcaccttgag tgacttctac
     9901 cattttatag tcaaattgct ttagttcgtt tggtttaacg ccgacactcg caaatgtata
     9961 atcaaagaac ttcacaatat tgttgcctaa gaagcctttg aattcaatag tgtcatttcc
    10021 agcaatttgt tcggcaacaa tacttgctgc acggtgagcg ccccaagcta aaggaacact
    10081 agccggtaga tcgacatgtc gataatgtga tgttgcaata tcgcctattg cataaatgtt
    10141 tggaacattt gtttcaaatt tatcgtttac cggtatgaaa ccttttcgat caagtttgat
    10201 atttgaactt tcgataaatt ttgaattggg gtgagtaccg acaccttcaa taatcatatc
    10261 gtaatgttca acttttcctg atttaaatgt aatttcattt ccattgatag cattaatttc
    10321 ctcatttaaa cggtatggaa tctcccgctt atctaattca tcaagtatag gttgattcat
    10381 gtcggcatcc attaatttat ttatcttatc agatcgatga attaaagtag ggtgtaaacc
    10441 acgttcataa agattttcaa gaacttctaa tgaaacataa cctgcaccta caaccaatac
    10501 tttatcaact tgatttgctt tgatgaattg atcgatagca tcagtgtctt ctaaatttct
    10561 aagtgtaaat gtaatatcac tttcaaagcc aaggctattt gcacttgcac cagggcttaa
    10621 aatgagttta tcgtaagatt cttcaaattg ttcgtttgtc tttctattta atacagatac
    10681 agtttgtctt tcatcattga ttgcaataac ttcatgataa gtttttactg taatttgctt
    10741 tctatcataa aatttttcag gtgtatacgc taaagcatat cttctatctt caacaacttc
    10801 gccaatgaca taaggcaatg cacaattagc aaagctcata tcacgatctt tttcaaaaat
    10861 aataatgtca ctttctttat ctaaacgtcg aatttggctg gcacatgttg caccgccagc
    10921 gactgctccg actacgacta ttttgggcat cgtaacttac ctcctattgt tttaaaatat
    10981 tataaaatga ttagttataa attaacagta atatctaata tttaaattaa agaaatcatt
    11041 caaatatcga ccaatgccat cttcattatt gttgaatgta atattgttcg ctacatcttt
    11101 aagttcttgc aaaccatttt ccatagcaac accgtgacgc gcgtactcaa tcatttcaat
    11161 atcattatct tcatcaccga atgcaataat attatttcgg tcaatattta aaaattgtct
    11221 aacttgctca atgcctcttg ctttattaat accaagtttt acaatttcaa tgacagggaa
    11281 tggtgcgccc cagcgtcgat gctcaatatg atcggcataa aaatgagtaa gcatattttt
    11341 gatttcaggt attttacttt cttcggcttc aattaaaatt gaggtagggg attctttcaa
    11401 gtggacaagt aaattaccag tttgaattct tggattaccc attgaaaaac cttcaaataa
    11461 tcttggatca tgattgttaa tgaaaacata atctttcact tctgctataa tattcgatac
    11521 ttgatattgt tgtaatcctt gaataatgtt ttgtgcgatg cctaaatcta aaatttcatg
    11581 gcaagttttg aagtttttat ctttagggtg atgtacgtaa gcgccattaa aattaacaat
    11641 tggtgtcgtt aaatttaatt catgataata catttgactt gcacgataag gtctgccagt
    11701 cgcaatcata atttggtgtc cacgttgttg taattcattt aatacttgtt tagtatatga
    11761 tgaaatttct ttgttatcgt ttaataatgt tccgtctaag tctagacata ttaaatgtgg
    11821 ttgcatatct ctctcctcat aagttgctta gttcatatag atacatcata gcattttatt
    11881 ggcgttttgt cggatatttg atatattatg aataatatga cattaaagag gtgatatgga
    11941 tggaagaggc attgaaagat agtatcttag gtgcattaga aatggtaatt gaccctgaat
    12001 taggaattga tatcgttaat ttgggtttag tatacaaagt gaatgttgat gatgaaggcg
    12061 tatgtacagt tgatatgact ttaacatcaa tgggatgtcc aatgggacct caaattattg
    12121 atcaagttaa aacagtatta gcagagattc ctgaaattca ggatactgaa gtgaatatcg
    12181 tatggagtcc accttggaca aaagatatga tgtcacgtta cgctaagatt gcacttggtg
    12241 tgagctaaca taaacttgca ttgcctgtag aatttctttt cgaaattctc tgtgtttggg
    12301 cccgcagtca atataacatt gtagagtata attcattgtg aataagctcc cttcaaagta
    12361 gacattgaaa aatgaaaatt ttgaagggag ccttatttat gttctagttt ctgattttat
    12421 tttgaaaatt tatatctaac ttgtttcaaa aaatatgaat taagacagtg acttgcactt
    12481 ccaagagtgt acaattcatt ttctagttga atattttctt gaaaattttt ataataaatt
    12541 taatttatat tacagttata ttacaataca taatacttca tagtttttaa gttgccgagt
    12601 tgcattacaa attggatttc gaagtaagaa tcatcattgg taattttttt aatttaacag
    12661 tattttagtc aatatgattt tgatgatgtc gtaattcaat aatatacata tgcatttcaa
    12721 tattctttta aacagcataa ctaaatttaa ttaagcatct aagaagaaca gttttaaaag
    12781 ggaagaatgc aaaaatgaac aaaacaaagg gttttacaaa gtataagaaa atgagatata
    12841 ttccagggct cgatggtttg agggcaatcg ctgttctagg aattattatt taccacttaa
    12901 ataagcaatg gttgacaggt ggctttttag gtgtggatac attttttgtg atctctggtt
    12961 atttaattac aagcttatta ctcaaagagt atgatgacac aggtatcatt aaattgaaaa
    13021 gcttttggat acgtcgttta aaacgtttat taccagcagt catagtttta ttaatggttg
    13081 tagggacagc aaccttatta ttaaaatcag ataatatcat tagggttaaa catgatatta
    13141 ttgctgcgat attttatgta tcaaactggt ggtatatagc aaaagatgtt aattattttg
    13201 agcaattttc atttatgcca ttaaagcatt tatggtcttt agcaattgaa gaacagtttt
    13261 acatattttt cccagttatt ttggttacat tattgttaac aattaaaaag cgatacaaaa
    13321 taggatttat tttttgggga gtatcaataa tttctttagg gttaatgatg tttatctata
    13381 gtattaatgg ggatcattca cgagtgtatt ttggtacaga tactagatta cagacattgt
    13441 tactgggtgt tattttagct tttttatggc caccgtttaa attgaaaaat gatccaccta
    13501 aagttgtaaa atatgttatt gatagcatag gtagtttatc atttatagta cttatattat
    13561 tatttttcat tattaatgat gagacgaatt ggatatatga tggtggtttc tatttaatat
    13621 ccatattaac gttatttatt attgctagtg tcgttcatcc atctacatgg atagcgaaga
    13681 tattttcaaa tccagtgtta gtatttatcg ggaaaaggtc ttatagttta tatttatggc
    13741 attttgcagt aattagtttc gtacatagtt actatgtaga cggacagata cctgtatatg
    13801 tgtactttat agatataagt ttaacaatta tatttgcaga gctatcatat cgctttatag
    13861 aaactccatt tagaaaagaa ggtattaaag ctttaaattg gcgaccttct tatataccac
    13921 aatttataag aatggcaatt gtagtaacct tgttaattcc atttatgttg attttagtag
    13981 gtgcattcaa taaatatggt aaagacatta ttggagaaaa agcgaatagc tttgatacca
    14041 caattgaaga taattattta atgcggatag caccaattga taacatacac attgatggct
    14101 tagtaagtga gaagaaaaag gaatcttccg acgtatataa taatattaaa cctcttttaa
    14161 tcggtgattc agtaatggtt gatatcggtg agtcatttaa gtcatcagtt cctaagtcta
    14221 gaattgatgg aaaagtaggg cgtcaattgt atcaaacctt acctttagtt aaagcgaatt
    14281 attcacaata taaaaaatca tctgatcaag tcgtattaga attaggtaca aatggcgact
    14341 ttactgtcaa acagctcgac gatttactta atcaatttgg aaaagccaag atttatttag
    14401 ttaatacacg tgttccaaga atttatgagg caaatgtaaa tcgattatta gctgacgcgg
    14461 cgaaacgaaa gtccaatgtc acattaattg attggtataa gcgatcacaa ggacatagtg
    14521 aatattttgc accagacggt gtacatttag agtacaaagg agtcctagct ttaaaagatg
    14581 aaatattaaa agcacttaaa aagaaataac atatgtttaa taagtctagt taatgtgtaa
    14641 cgtaacatta gctagatttt tttattcaaa aaaatattta caaatattag gaaatttaag
    14701 tgtaaaagag ttgataaatg attatattgg gactataata taattaaggt caaagaaagt
    14761 caaagtcaaa agtgcaactt gaccaactct acaaattaga ggtgaaatta tatggatata
    14821 aataaaatga catatgctgt tcaaagtgct ttacaacaag cagttgaact gagtcagcaa
    14881 cataaattac aaaatataga aattgaggca attttaagcg ctgccttaaa tgaaagtgaa
    14941 agcttatata aaagtatttt agaacgagca aatattgagg tagatcaatt aaacaaagct
    15001 tatgaagaca aactaaacac gtatgcatct gtagaaggtg acaatataca atatggtcaa
    15061 tatattagcc aacaagcaaa ccaattgata actaaggctg aatcatacat gaaagaatat
    15121 gaagatgaat atatttcaat ggagcatatt ttacgttcgg caatggacat tgatcaaaca
    15181 acaaaacatt atataaataa taaagtagaa gttatcaaag aaattattaa aaaagtaaga
    15241 gggggaaatc acgtgacatc acaaaatcca gaagttaatt acgaagcatt agctaaatat
    15301 ggccgcgact tagtagaaga agttagacaa ggtaaaatgg atcctgttat aggaagagat
    15361 gaagaaattc gaaatacgat tcgtatttta agtcgtaaaa ctaaaaacaa ccctgtgctc
    15421 attggtgaac caggtgttgg taaaactgca attgttgaag gattagcgca acgtatagtt
    15481 aagaaagatg tgccagaatc attattagat aaaactgttt ttgagttaga tttaagcgca
    15541 ttagtagcgg gcgctaaata tcgtggtgaa tttgaagaga gattaaaagc agtcctaaaa
    15601 gaagttaaag agtctgatgg tagaattata ttatttattg atgaaatcca tatgcttgta
    15661 ggtgctggta aaacagatgg tgccatggat gcaggcaaca tgctaaaacc aatgttagca
    15721 cgaggagagt tacattgtat tggtgcaaca actttaaatg aatatcgaga atatattgaa
    15781 aaagattcgg cattagagcg tcgtttccaa aaagtagcag ttagtgagcc tgatgttgaa
    15841 gatacaattt caattttacg tggtttaaaa gaacgatatg aagtgtatca tggtgtgcgt
    15901 attcaagata gagccttagt tgctgccgct gaattgtctg atcgttacat cactgatcgt
    15961 tttttaccag ataaagcgat tgatttagtt gaccaagcat gtgcaacaat tcgtacggaa
    16021 atgggatcaa atccaactga attggatcaa gttaatagac gtgtcatgca attagaaatt
    16081 gaagaaagcg cacttaaaaa tgaatctgac aatgcgagca aacagagatt acaagaacta
    16141 caagaagagc ttgccaatga aaaagagaaa caagcagcac ttcaatctcg tgtagaatca
    16201 gaaaaagaaa aaatagcaaa tttacaagaa aaacgtgcgc aactagatga aagtagacaa
    16261 gcgttggaag atgcacaaac aaataacaat ttagaaaaag ctgctgaact acaatatgga
    16321 acaattcctc aattggaaaa agaacttaga gaattagagg ataatttcca agatgagcaa
    16381 ggtgaagata cagatcgaat gattcgtgaa gttgtaacag acgaagaaat tggcgatatt
    16441 gtcagccaat ggacaggcat accagtttca aaattagttg aaacagaacg tgaaaaatta
    16501 cttcacttaa gtgacatctt gcataaacgt gttgtaggtc aagataaagc ggttgacctg
    16561 gtttcagatg cagtagttag agcaagagca ggtattaaag atccaaacag acctattggt
    16621 agtttcttat tcctaggtcc aactggagta ggtaaaactg aattagctaa atcattagct
    16681 gcatcattat ttgattctga aaaacatatg attcgtattg atatgagtga atatatggaa
    16741 aaacatgcag tatcaagatt gataggggca cctccaggat atattggaca tgatgaaggg
    16801 ggtcaattaa ctgaagcggt tcgtcgtaat ccatactcag ttattttatt agatgaggtt
    16861 gaaaaagcgc atactgacgt ctttaatgta ttattgcaaa ttttagatga aggccgttta
    16921 actgattcta aaggacgtag cgttgatttt aaaaatacta ttattattat gacaagtaat
    16981 attggatctc aagttttatt agaaaacgta aaagagactg gtgaaattac agaatcaaca
    17041 gaaaaagctg ttatgacaag tttaaatgca tatttcaaac cagaaatttt gaatcgtatg
    17101 gatgatatcg tattatttaa accattatct attgatgaca tgagtatgat tgtagataaa
    17161 atcttaacgc aattaaatat aagattatta gaacaacgaa tctcaattga agtttctgat
    17221 gatgctaaag cttggctagg tcaagaagct tatgaacctc aatacggtgc aagaccatta
    17281 aaacgttttg tacaacgcca aattgaaaca ccattagcac gtatgatgat taaagaggga
    17341 ttcccagaag gtacaacgat taaagttaat ttaaattcag acaataactt aacgtttaat
    17401 gttgaaaaaa ttcatgaata aaattgaacc aagaatgtga tgattaatcg cattcttggt
    17461 tttttagatt tgaaatttat tataattgtt cattggcaat atacttttta aatgatttaa
    17521 taaacaccaa tatttctggg ctttcttttt tgctgtatat atatgtaaat gaaataggtg
    17581 cttgtaaaat tttcgtgtta ataaccgaaa tattgtaatc actatttgtt gttatataaa
    17641 gaggcaggaa tgatatacct tgattcattt cgattaattt aattgaagta tgcacatcat
    17701 tgatagatag aaattgtgct ttttcataaa tatttaaaat attattttta agtgatgacc
    17761 aatattctgg atggttatca cttattattt tgtatttttc aaataaagat gcctcagtta
    17821 gaagatgatt attctcttta ttgggagcaa tcaatacaat tttaccttcg catacttttt
    17881 cagaatgaac ttctcttagt ttaggttgat ttctgctaat cccgatgtca tacgtatgat
    17941 tattaatatc tttttcaata ttttcatttt tgacatgaag agaaacatcg ataaaaggat
    18001 gctcgttaaa gaaagatttt aaaaatttgg gcataatgaa tgtcgcgata tatgaagaca
    18061 cgacaacatt taatttcgat tgaaacatcg tttttttaag ttggatatgt ttgatgccac
    18121 tttcatattg ttcaataaaa ctttgcgcaa ttggaagaaa tgtatgacca tcttcagtca
    18181 agataatttg atttttataa gtttcaaata gtttcacatt gagatgctgt tctaaatttt
    18241 taatttgctt atgtatagaa ggtatagtga gattaatttc ttcactagct aatcgatagt
    18301 ttaacgtctt cgctaaaatg acaaatgtat agtaccaatc taaattcatg aacttacctc
    18361 tttcaaaaaa gttaataatt cattagataa tatttaattt ttattgcata aaaataatat
    18421 taatattata tcagaggtta tcgagggggt gaagtaatat gattcgaatt caagataata
    18481 caataaggga tggtatgcaa caaagtaatg ttcgaaaaag tctaattata aaaaaagaag
    18541 tattgaaaca aattaacaag ttaaatataa attctgttga agtaggcatg tgtacaacta
    18601 tcgaggatga atttaatatt catcaattca gagacatttt aagtcctgaa aaagaattag
    18661 tagtattgac taggcttaat gaaaaagaaa taaaaaaaat agtcaaattg aaaattcata
    18721 atttagtggt aaaaatacta ttgccaatat ctgacttgca tataaaagaa aagcttaatt
    18781 tttcaaataa atattatatt cagaaaatca aagactgctt ggatatatta aagaaagata
    18841 aaaaaggagt agatatttgt tttgaagatg gaacaaggac ttctagagaa aaattgaaag
    18901 aatacatgga aattatttca aaatatcaag ttagaacagt tacatttgcg gacactgtag
    18961 gatgttcgac accattagaa tacggagata tttttaatta ctttgtaaaa aaatattcta
    19021 acataatttt ttctgctcat tgtcataacg atctagggtt ggctactgca aatacattag
    19081 ctgcaatttt aaatggtgca aagcaaatag aaactacatt tttgagaatt ggtgagagag
    19141 cgggtaatgc tcctattgag gaaataatta ctattttgac aaaaaaacaa atagaaagta
    19201 cggaattcac tttacccgac gtatataaaa ctagtattaa tatttctaaa attctcgatt
    19261 ttcaaatatc agaaaacaaa cctataattg gtgaaaatat atttaaacat gaatcaggaa
    19321 ttcatcaaga tggtactaaa aaaaatataa atatgtatca atatttagtt cctagtgatt
    19381 taggattgaa aaattcacaa gttgttcagt ttccaataag taatatttct agtaagaaaa
    19441 tcttgcacaa taaatttaaa tcaatagtta acactgaaga aattgatgaa aatatttctt
    19501 tctataaact tgtgaacaag ttttcacctg aagtagcacc tgaagataca gtggatttac
    19561 ttcagataat aaaaaggagg agtaaagatg gaaattttaa atgattctgt aaaaagtttt
    19621 gaaaaacttt atttacaatt acaagatggt tttccagtga ttgttcctac tgatactaat
    19681 tataatttat gtagtttacc taacaacgat ctttgtatcg ataaaatatt tgaatataaa
    19741 aagcgatcga aagataagcc attatcatta tttattgata agccagagga ttggaaattg
    19801 tatggagata atcaaaatac ggaaatagtt gataaactag ttgaaatatt ttggcctgga
    19861 ccattaaata ttattttaaa aaataaaaca agctataatt atatgctcaa taattcagat
    19921 agtatagcta taggatgtgt acaaaataaa acgatgagaa gatttatttc atatattaat
    19981 tcacctatag caattacttc agcaaatata tctggaactg ccgatgatat tttaataact
    20041 gaaaatgaag caattaagca catgggcgaa aatgtaagat atatgctaag aagtcaaaat
    20101 aaaactaact ataaaacatc tagtacgatt attaaagtga cagataataa aattgaatta
    20161 ttaagagaag gagatataaa gtttgaagaa ataaaagaaa gactaggtac aggtattatt
    20221 tatgaataag ttaatacttg ggatttattt ataccgaatt ttttcacgag catactttta
    20281 tttaccgttt ttattaattt actttttgat tcaaggttat tccataatac aattagaaat
    20341 attaatggcg tcttatggca ttgcagcatt tttattctct ctatacaaag agaagtgttt
    20401 taaaatttgt aacttaaaag attctaataa attagttgtt agtgaaatat tcaaaatcat
    20461 cggttt
//
LOCUS       DALAGD010000056        19736 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.056, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000056 DALAGD010000000
VERSION     DALAGD010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19736
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.056"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            46..159
                     /locus_tag="O2701_001626"
     CDS             46..159
                     /locus_tag="O2701_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293517.1"
                     /translation="MNGILIKCLNIGGQYYLQTRMLSNVDNAEKIISRNSI"
     gene            541..801
                     /locus_tag="O2701_001627"
     CDS             541..801
                     /locus_tag="O2701_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293518.1"
                     /translation="MIIMRGYNGRYCSWTNTAIQCDYFNKVRQVKKDGKSDRLSNNIN
                     SNIIYRVLEKCITLYNVCSRCKRALILSEHSKKNIFNIKKAV"
     gene            811..1308
                     /locus_tag="O2701_001628"
     CDS             811..1308
                     /locus_tag="O2701_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCZ4293519.1"
                     /translation="MNNRNVYDIEVSDYKGLTYKLEAFRGKVILVVNTATECIYSEQL
                     KKLETLFQKYKDRGFVVLSFPNNNFDNRQPGSNEEILKISREKFGVTFPVLAKISVNG
                     NDEHPLFTHLKNEQPGIFGSPIKWNFTKFIIDRQGNVVNRFLPMEDPLDISTNIEILL
                     QESSS"
     gene            complement(1416..1508)
                     /locus_tag="O2701_001629"
     CDS             complement(1416..1508)
                     /locus_tag="O2701_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293520.1"
                     /translation="MSVINAYSNASLLFFIYLLFIMYQISLSVK"
     gene            1703..3583
                     /locus_tag="O2701_001630"
     CDS             1703..3583
                     /locus_tag="O2701_001630"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HCZ4293521.1"
                     /translation="MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLANSNDT
                     TSDSNQQQLETEVWQQSQISEEQATSTTYMLQNKEPHIEANQRHVTVLYGSESGNAMR
                     LAEIFSERLSDIGHQVVLMSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFL
                     EDDNAPNLNHVRYSVLALGDQTYEFFCQAGKDVDVLLENLGAERICKRVDCDIDYEED
                     AEKWMADIINIIDTTSEGIQSESVISESIKSAKEKKYSKSNPYQAEVLANINLNGTDS
                     NKETRHIEFLLDDFSESYEPGDCIVALPQNDPELVEKLIFMLGWDPQSPVPINDHGDT
                     VPIVEALTSHFEFTKLTLPLLKNADIYFDNEELSERIQDESWAREYVINRDFIDLITD
                     FPTIELQPENMYQILRKLPPREYSISSSFMATPDEVHITVGTVRYQAHGRERKGVCSV
                     HFAERIKPGDIVPIYLKKNPNFKFPMKQDIPVIMIGPGTGIAPFRAYLQEREELGMTG
                     KTWLFFGDQHRSSDFLYEEEIEEWLENGNLTRVDLAFSRDQEHKEYVQHRIMEESKRF
                     NEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKERHISQEEAELLLRQMKQQQRYQRD
                     VY"
     gene            3646..4251
                     /locus_tag="O2701_001631"
     CDS             3646..4251
                     /locus_tag="O2701_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HCZ4293522.1"
                     /translation="MNMPLMIDLTNKNVVIVGGGVVASRRAQTLNQYVEHMTVISPTI
                     TEKLQNMVDNGVVIWKEKEFEPSDIVDAYLVIAATNEPRVNEAVKKALPEHALFNNVG
                     DASNGNVVFPSALHRDKLTISVSTDGASPKLTKSIMAELEALYPPSYSSYIDFLYTCR
                     QKIKVLDITYNEKQQLLSQIVSQEYLNHDKQAQFLAWLDVR"
     gene            4327..4422
                     /locus_tag="O2701_001632"
     CDS             4327..4422
                     /locus_tag="O2701_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293523.1"
                     /translation="MGGFVNSLIMKDLSDNDMRFKYEYYNREKDT"
     gene            4744..6114
                     /locus_tag="O2701_001633"
     CDS             4744..6114
                     /locus_tag="O2701_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HCZ4293524.1"
                     /translation="MNSDNMWLTVMGLIIIISIVGLLIAKKINPVVGMTIIPCLGAMI
                     LGYSVTDLVGFFAKGLDQVINVVIMFIFAIIFFGIMNDSGLFKPLVKRLILMTRGNVV
                     IVCAMTALIGTIAQLDGAGAVTFLLSIPALLPLYKALNMNKYLLILLLALSAAIMNMV
                     PWGGPMARVAAVLKAKSVNELWYGLIPIQIIGFILVMLFAVYLGFKEQKRIKKAIERN
                     ELPQTQDIDVHKLVEVYERDQDVRFPVKGRARTKSWIKWVNTALTLAVILSMLINIAP
                     PEFAFMIGVSLALVINFKSVDEQMERLRAHAPNALMMAAVIIAAGMFLGVLNETGMLK
                     AIATNLIKVIPAEVGPYLHIIVGLLGVPLDLLTSTDAYYFAVLPIVEQTAGQFGVPSV
                     STAYSMVIGNIIGTFVSPFSPALWLAIGLAEANMGTYIKYAFFWIWGFAIVMLVIAML
                     MGIVTI"
     gene            6361..8211
                     /gene="nrdD"
                     /locus_tag="O2701_001634"
     CDS             6361..8211
                     /gene="nrdD"
                     /locus_tag="O2701_001634"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCZ4293525.1"
                     /translation="MNQIEEALTGLISKDPAIVNENANKDSDTFSTMRDLTAGIVSKS
                     YALNHLLPKHVADAHQRGDIHFHDLDYHPFQPLTNCCLIDAKNMLHNGFEIGNANVTS
                     PKSIQTASAQLVQIIANVSSSQYGGCTVDRVDELLSTYARHNEEQHRNIAKQFVKESE
                     IDRYVDQQVTKDINDAIESLEYEINTLYTSNGQTPFVTLGFGLGTDHLSRKIQQAILN
                     TRIKGLGKDRTTAIFPKLVFSIKKGTNFSPQDPNYDIKQLALKCSTKRMYPDILNYDK
                     LVEILGDFKAPMGCRSFLPSWKDAEGHFENNGRCNLGVVTLNLPRMALESAGNMTKFW
                     EIFYERIDVLHDALLYRINRLKDAVPNNAPILYKSGAFNYKLKETDDVAELFKNKRAT
                     ISMGYIGLYETATVFYGPDWETSQEAKAFTLEILKEMKRYQTKWTELYDIWFSIYSTP
                     SESLTDRFCRLDQERFGDIKDITDKGYYQNSFHYDVRKDVTPFEKLDFEKDYPYYASG
                     GFIHYCEYPKLQHNLKALEAVWDYSYDKVGYLGTNIPIDHCYECDYDGDFEATEKGFK
                     CPNCGNDNPKTVDVVKRTCGYLGNPVQRPVIKGRHKEICARVKHMKAPKE"
     gene            8208..8744
                     /gene="nrdG"
                     /locus_tag="O2701_001635"
     CDS             8208..8744
                     /gene="nrdG"
                     /locus_tag="O2701_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HCZ4293526.1"
                     /translation="MILLDIKQGQGYIAKIESNSFVDGEGVRCSVYVSGCPFNCVGCY
                     NKASQKFRYGEKYTDEILAEILDDCDHDYISGLSLLGGEPFCNLDITLNLVKAFRARF
                     GNTKTIWVWTGFLYEYLANDCTERRELLSYIDVLVDGLFIQHLFKPDLPYKGSLNQRI
                     IDVQQSLSHARMIEYIVS"
     gene            8844..8954
                     /locus_tag="O2701_001636"
     CDS             8844..8954
                     /locus_tag="O2701_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293527.1"
                     /translation="MIEYDLTIILIPILKVAYPFFKKVEREKWYFSGKEV"
     gene            complement(8970..9131)
                     /locus_tag="O2701_001637"
     CDS             complement(8970..9131)
                     /locus_tag="O2701_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293528.1"
                     /translation="MAIIEYLYNFSRNKQNLNSAKITVLSYDLTNLTNFTRMAFKNIY
                     TLLTNIYLL"
     gene            9263..10885
                     /locus_tag="O2701_001638"
     CDS             9263..10885
                     /locus_tag="O2701_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCZ4293529.1"
                     /translation="MKKEKVMDWTTFIGTVAVLLFAVIPMMAFPKASEDIITGINSAI
                     SDSIGSIYLFMGLAIFCFVMYIAFGKYGNVTLGKASDKPEFNTFTWAAMLFCAGIGSD
                     ILYWGVIEWAFYYQVPPNGAKSMSDEALQYATQYGMFHWGPIAWAIYVLPALPIGYLV
                     FVKKQPVYKISQACRPILKGQTDKFVGKVVDILFIFGLLGGAATSLALGVPLISAGIE
                     RLTGLDGKNMILRSAILLTITVIFAISSYTGLKKGIQKLSDINVWLSFVLLAFIFIIG
                     PTVFIMETTVTGFGNMLRDFFHMATWLEPFGGIKGRKETNFPQDWTIFYWSWWLVYAP
                     FIGLFIARISKGRRLKEVVLGTIIYGTLGCVLFFGIFGNYAVYLQISGQFNVTQYLNT
                     HGTEATIIEVVHHLPFPSLMIVLFLVSAFLFLATTFDSGSYILAAASQKKVVGEPLRA
                     NRLFWAFALCLLPFSLMLVGGERALEVLKTASILASVPLIVIFIFMMISFLIILGRDR
                     IKLETRAEKLKEVERRSLRIVQVSEEEQDDNL"
     gene            11149..11835
                     /locus_tag="O2701_001639"
     CDS             11149..11835
                     /locus_tag="O2701_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCZ4293530.1"
                     /translation="MTNQFKNKQSKLHDSLESITKNLYATPTSELPFDNRFLFKSFIL
                     KRETGNIVIYHSGHLGDSQQDIASLGGVSKVLMNHDHESIGGSNQVEAPYFIHENDVA
                     ALKHKISVQKQFSNRVMLDKDLEVIPAPGHTPGTTLFLWDDGHHRYLFTGDFICFEGK
                     RWRTVILGSSDREKSIQSLEMVKELDFDVLVPWVTIKDEPLVYFVENEYEKREQIQNI
                     IDRVREGENS"
     gene            complement(12023..12586)
                     /locus_tag="O2701_001640"
     CDS             complement(12023..12586)
                     /locus_tag="O2701_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HCZ4293531.1"
                     /translation="MTRSQNKQQQLEQAKDIVINSIGQTMDLYGTNRSVGNLYGTMVF
                     EGSMTLDEMRHQLQMSKPSMSAGVKKLQEFDIVKQQFTRGSRKQHFIAEKDFFIFFRN
                     FFTKKFQREIDINVEAVKDAQAIINSLLESSDLTEAETQEAQKIKSQLDHTHVYYEWL
                     EQLTEAIESGEIFKYFPIPEQPSDSEN"
     gene            complement(12675..12770)
                     /locus_tag="O2701_001641"
     CDS             complement(12675..12770)
                     /locus_tag="O2701_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293532.1"
                     /translation="MKNVSIYKTTQELSFVSSLDFYLTKHLTLML"
     gene            12839..14329
                     /gene="betB"
                     /locus_tag="O2701_001642"
     CDS             12839..14329
                     /gene="betB"
                     /locus_tag="O2701_001642"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HCZ4293533.1"
                     /translation="MELLKHLSQRQYIDGEWVESANKNTRDIINPYNQEVIFTVSEGT
                     KEDAERAILAARRAFESGEWSQETAETRGKKVRAIADKIKEHREALARLETLDTGKTL
                     EESYADMDDIHNVFMYFAGLADKDGGEMIDSPIPDTESKIVKEPVGVVTQITPWNYPL
                     LQASWKIAPALATGCSLVMKPSEITPLTTIRVFELMEEVGFPKGTINLILGAGSEVGD
                     VMSGHKEVDLVSFTGGIETGKHIMKNAANNVTNIALELGGKNPNIIFDDADFELAVDQ
                     ALNGGYFHAGQVCSAGSRILVQNSIKDKFEQALIDRVKKIKLGNGFDADTEMGPVIST
                     EHRNKIESYMDVAKAEGATIAVGGKRPDRDDLKDGLFFEPTVITNCDTSMRIVQEEVF
                     GPVVTVEGFETEQEAIQLANDSIYGLAGAVFSKDIGKAQRVANKLKLGTVWINDFHPY
                     FAQAPWGGYKQSGIGRELGKEGLEEYLVSKHILTNTNPQLVNWFSK"
     gene            14590..16299
                     /gene="betA"
                     /locus_tag="O2701_001643"
     CDS             14590..16299
                     /gene="betA"
                     /locus_tag="O2701_001643"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HCZ4293534.1"
                     /translation="MSNKNKSYDYVIIGGGSAGSVLGNRLSEDKDKEVLVLEAGRSDY
                     FWDLFIQMPAALMFPSGNKFYDWIYSTDEEPHMGGRKVAHARGKVLGGSSSINGMIYQ
                     RGNPMDYEGWAEPEGMETWDFAHCLPYFKKLEKTYGAAPYDKFRGHDGPIKLKRGPAT
                     NPLFQSFFDAGVEAGYHKTPDVNGFRQEGFGPFDSQVHRGRRMSASRAYLHPAMKRKN
                     LTVETRAFVTEIHYEGRRATGVTYKKNGKLHTIDANEVILSGGAFNTPQLLQLSGIGD
                     SEFLKSKGIEPRVHLPGVGENFEDHLEVYIQHKCKEPVSLQPSLDIKRMPFIGLQWIF
                     TRTGAAASNHFEGGGFVRSNNEVDYPNLMFHFLPIAVRYDGQKAAVAHGYQVHVGPMY
                     SNSRGSLKIKSKDPFEKPSIRFNYLSTEEDKKEWVEAIRVARNILSQKAMDPFNGGEI
                     SPGPEVQTDEEILDWVRRDGETALHPSCSAKMGPASDPMAVVDPLTMKVHGMENLRVV
                     DASAMPRTTNGNIHAPVLMLAEKAADIIRGRKPLEPQYIDYYKHGVHDENEGAIEVKP
                     YAK"
     gene            16577..16789
                     /locus_tag="O2701_001644"
     CDS             16577..16789
                     /locus_tag="O2701_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HCZ4293535.1"
                     /translation="MSFYEYIQTFKDDKTPLGELAIWIKEDDSFPKQEKLTENILSYF
                     HQMSNIDHEFLEIVKRSLSLYDQLKS"
     gene            17070..17513
                     /locus_tag="O2701_001645"
     CDS             17070..17513
                     /locus_tag="O2701_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCZ4293536.1"
                     /translation="MKIYDEYRSYVIEELDDCLTIQKNNDTAYYDVLEAINDLSNDSL
                     CVLNHLYINEGQEETFEQKFLRRNKHLKNVDGFKALRFLRPRTAGRHYIIITLWENRQ
                     AFYHWQNSAEYKHTHKHRGTSKGADVKIINRELSYNIRIELADMV"
     gene            17707..19305
                     /locus_tag="O2701_001646"
     CDS             17707..19305
                     /locus_tag="O2701_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HCZ4293537.1"
                     /translation="MQKSALFAPEKYNIISEIEKYSHIPDKKAILYHNTEGEDISVTY
                     QQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRT
                     KDLQYRITHGEINAVIALEPFTVEFEKIKEYDALTKFVIAGQKDGWISLEAEKENASN
                     KLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWAT
                     AAPGWQKWVWSPFLSVLGSGATAFVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMA
                     KLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIGFLKDT
                     PQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDLPALFKGYYKEPERTAKAQ
                     TGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVA
                     KPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNS
                     GKIRRVELRDAEIEKWQQQKDSNQ"
     gene            complement(19365..19694)
                     /locus_tag="O2701_001647"
     CDS             complement(19365..19694)
                     /locus_tag="O2701_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293538.1"
                     /translation="MAYSILNLYSILIQYKCKINHKKDIQYLHPRKTLQLPLILKIIL
                     NLALIGIVIIVLTHTEFQLNAGDSNVFLLNWDIRGLTSYIFSFLILVALVSKLILIFI
                     TRRQRTS"
BASE COUNT         6930 a         2840 c         3809 g         6157 t
ORIGIN      
        1 atatgaatag aaatatcaat aagaaaaata atatgattaa aaatgatgaa tggcatactt
       61 ataaagtgtc taaatattgg cggtcaatat tacttacaaa cacgaatgtt aagtaatgta
      121 gacaatgctg aaaaaataat aagtagaaac tctatataga atgttcgttt ttatttttaa
      181 taattgaata atcattattg atgatttaat atgtattcgg tcgcacacga tacaaagtga
      241 cttgttttaa tgatatgcaa taatatgacg tttatcgtcg cgtgtaacaa tgtatgagtg
      301 tgtcagttta attttaaaag ggttataagg atatttaata agataaagga gggcttaata
      361 aacaatcaca tgtgaagttt ttaaggatta tatgagttgg gcagctcttt aatccatagt
      421 tataacgaat tttaaattgt tagatctaac gtatggtatc agtatgtatg agtttaactt
      481 aagtattaat ctatagttta aagagcggtc taattgtctt tttaaaaata gaaaataagg
      541 atgattataa tgaggggtta taacggaaga tattgtagct ggactaatac agctatacaa
      601 tgtgattatt ttaataaagt aagacaggtg aaaaaagacg gtaaatcgga taggttgagc
      661 aacaacataa actcgaacat tatttatcgt gtgctcgaaa aatgtatcac gctatataat
      721 gtgtgttcac ggtgtaaacg cgcacttatt ttatcagaac attctaaaaa gaacatattc
      781 aatataaaaa aggctgtgta ggggtgtctt atgaataata gaaatgtgta tgatatcgaa
      841 gtaagtgatt ataaaggctt aacttataaa ttagaagcat ttagaggtaa agtgatttta
      901 gttgttaata ctgcaacaga atgtatatat agcgaacaat tgaaaaaact agagacttta
      961 tttcaaaaat ataaggatcg tgggtttgta gtgttgagtt ttccgaataa taattttgac
     1021 aatcgacaac caggttctaa tgaagaaatc ttgaaaatta gtcgtgagaa atttggtgta
     1081 acatttccag tgttagctaa aatatctgtg aacgggaacg atgaacatcc gttatttacg
     1141 catttaaaga atgaacaacc aggaatcttt gggtccccaa taaaatggaa tttcacaaaa
     1201 tttataatcg atcgacaagg aaatgttgta aatcgattct tgccaatgga agatccattg
     1261 gatatatcga caaatataga aatattattg caagaatctt catcttaaat ttaaatttga
     1321 gcgcttagta tgcaaataac acaagagcct cttaatgatg tactaatagt atgttattag
     1381 gaggcatttt atatttagaa aatcagtaat ttgacctatt ttactgaaag gctgatttgg
     1441 tacataataa atagtaaata aataaaaaat aacaacgatg cattactgta tgcattaata
     1501 actgacacga cataatcaaa gttttgtctg aattactgat aatgtcattc aagcaacatg
     1561 aaggttgtaa atatttgcaa gtataactat cagacacaac tcaagtttgt tttaatttat
     1621 accatacata ttaaaaaatt gactaaacta tcgataaaaa aagagtcaat catctatttc
     1681 aagtttattg aggtgaaaac atttgaaatt aaatacatct aatagtccat ttacagaaaa
     1741 acaagtcaca gagattaaca atctgctgca aacattaaca gagagccaac aacagtggct
     1801 tagtggtttt ttactagcta atagtaacga tacgacaagt gatagtaatc aacaacaatt
     1861 agagacagaa gtgtggcaac aatcacaaat atcagaagaa caagcaactt caacaacgta
     1921 tatgttacaa aataaagagc cacatatcga agctaatcag cggcatgtta cagtgctata
     1981 tggttctgaa tcaggtaatg ccatgcgttt agctgaaatt ttttcagaac gtttaagtga
     2041 tatcggacat caagttgttt tgatgtcaat ggatgaatat gatacgacaa acatcgcgca
     2101 gttagaagat ttatttatta ttacgtctac tcatggtgaa ggagaaccgc ctgataatgc
     2161 atgggatttc tttgaatttt tagaagacga taacgcacct aatttaaatc atgtgagata
     2221 ttcagtacta gctttaggtg atcaaacata tgaatttttc tgtcaagccg gtaaagatgt
     2281 agatgtttta ctagaaaatc taggcgctga gcgtatatgt aagcgtgtag attgtgatat
     2341 tgattatgaa gaagacgcag aaaagtggat ggcagacatc attaatatta ttgataccac
     2401 atcagaaggt attcaaagtg aatcggtgat aagtgaatca attaagtctg ccaaagaaaa
     2461 gaaatattct aaatcaaatc cataccaagc agaagtatta gcgaatatca atttaaatgg
     2521 taccgattca aataaagaaa cacgacatat agaattttta cttgatgatt ttagtgaatc
     2581 atatgaacca ggagattgta tagtagcatt accgcaaaac gaccctgaat tggttgaaaa
     2641 actaatattc atgttaggtt gggatccgca atctccggtg ccaattaatg atcatggtga
     2701 tacagttcct attgttgaag cactaacatc acattttgaa tttactaaat taacattgcc
     2761 attattgaaa aatgcagata tctattttga caatgaagaa ttatctgaac gtattcaaga
     2821 tgagtcatgg gcgcgtgaat atgttataaa tcgggacttt atagatttaa taacagattt
     2881 tccaactata gaattacaac ctgagaatat gtatcaaatc cttagaaaat taccaccaag
     2941 agagtattcg atttctagta gttttatggc aacgccagat gaagtgcata ttaccgttgg
     3001 tacggttcgt tatcaagcac atggacgtga gagaaaaggt gtatgctcgg ttcattttgc
     3061 tgagcgaatt aaaccaggcg atatagtacc aatttatttg aagaaaaatc cgaacttcaa
     3121 atttccgatg aagcaagata taccggttat tatgattgga ccaggtactg gaattgctcc
     3181 ttttagagca tatttacaag aacgtgaaga acttggtatg actggaaaaa catggttgtt
     3241 ctttggtgat caacaccgta gttctgactt tttatatgaa gaagaaatag aagaatggct
     3301 tgaaaatgga aacttaacac gcgtagattt agcattttca agagaccaag aacacaaaga
     3361 atatgtacag catcgtataa tggaagaaag taaacgtttc aatgaatgga ttgagcaagg
     3421 cgcagcaatc tatatttgtg gcgatgaaaa atgtatggcg aaagatgtcc atcaagccat
     3481 taaagatgta ttggtaaaag aacgtcatat ttctcaagaa gaagcagagt tattattgcg
     3541 acaaatgaaa caacaacaac gctatcaacg tgatgtttat tagcgattgg tgttaaatat
     3601 tttaaggtgt aatgatgtaa aaagatataa aggatgttgc tcaacatgaa tatgccatta
     3661 atgatagatt taacaaataa aaatgtcgtc atagttggtg gaggcgtcgt tgcaagtcgt
     3721 cgggcacaaa cattaaatca atacgttgaa catatgacgg tcatcagtcc gacaatcact
     3781 gaaaaacttc aaaatatggt agataacggt gtcgtcatat ggaaagaaaa agaatttgaa
     3841 ccaagcgata ttgtagacgc gtatctagtt attgcagcaa ccaatgagcc acgtgtcaat
     3901 gaagcggtaa aaaaagcctt acctgagcat gcccttttta ataatgttgg agatgcatca
     3961 aatggcaatg ttgtatttcc aagtgcacta caccgcgaca agctaactat cagtgtatca
     4021 actgatggtg cgagtcctaa gttgacaaaa tcaattatgg cagagcttga ggcgttatat
     4081 ccaccatcat acagttcgta tatcgacttt ttatatactt gccgacagaa aataaaagta
     4141 cttgatataa catataacga aaagcaacag ttactgtcac aaattgtgtc acaagaatat
     4201 ttaaatcatg acaaacaagc tcaattttta gcgtggttgg atgtaagata ataatagcgg
     4261 accgtctaac cgtctaaggt aagtcttctt attttaactt taacgcttaa tcattgaaat
     4321 taagacatgg gcggctttgt gaatagtcta ataatgaagg atttaagcga taatgatatg
     4381 cgttttaaat atgaatatta caatagagaa aaagatacgt agaacaaact taataaaata
     4441 ggtggataaa ttgaaatctg gttgaagtcg ttactatcat agcgaccttt agccagattt
     4501 tttgtgcaat agaaagcaat aataaaaatg atagatcaaa atgaaataca ggacaggata
     4561 tacaaggatt agtcatgcca tgttatcaag taggaaaatc aaacttcact attgatagtt
     4621 acgcaaaaaa gatttttttg ataaaatgag ataacttaaa tataaaaaat tatattaatt
     4681 ataatattta agttaaagag ggggattatg taaattgtat taaaagtgga gggagaaaat
     4741 aatatgaata gtgataatat gtggttaaca gtaatggggc tcattattat tatttcaatt
     4801 gtaggtttac tcattgccaa aaagataaat ccagttgtag gtatgacaat cataccttgc
     4861 ttaggggcaa tgattttagg atatagtgtg acagatttgg ttggattttt tgctaaaggg
     4921 ttagatcaag tcatcaacgt tgttattatg tttatctttg ccattatttt ctttggcatc
     4981 atgaacgata gtggtttatt caagccgctt gtcaaacgct taatattaat gacacgaggc
     5041 aatgtcgtca ttgtctgtgc aatgacagct ttaattggca caatagccca attagatggg
     5101 gccggtgcgg taacattttt gctttctatt cctgcattat tacctttata taaagcgtta
     5161 aatatgaata aatatttatt gattttacta ttagcattaa gcgcggcgat tatgaacatg
     5221 gtaccttggg gaggtccaat ggctcgtgta gctgcagtgt taaaagccaa aagtgtcaat
     5281 gaattatggt atggattaat acctattcaa ataataggtt tcattcttgt tatgttgttt
     5341 gcggtatatc ttggatttaa agaacagaaa cgtatcaaaa aagcaataga gagaaatgaa
     5401 ttaccgcaaa cacaagatat agatgtacat aaattagttg aagtatatga acgagatcaa
     5461 gatgtaaggt ttcctgtaaa aggacgtgca agaacaaaat catggataaa atgggtgaat
     5521 acagctttaa ctttagctgt tattctatcg atgttaataa atattgcgcc acctgaattt
     5581 gcattcatga taggtgtttc gttggcactt gttattaatt ttaaatcagt ggatgaacaa
     5641 atggaacgat taagagcgca tgcgccgaat gcattaatga tggctgcagt gattattgca
     5701 gcaggtatgt ttttaggtgt actaaatgaa accggtatgc ttaaagcgat tgcgaccaat
     5761 ttaatcaaag tgattcctgc agaagtagga ccatacttgc atattattgt aggtttactt
     5821 ggcgtaccat tagatttact aactagcaca gacgcttatt attttgctgt gttaccgatt
     5881 gtcgaacaaa cagcagggca atttggtgta ccgtctgtat caacagctta ttcaatggtc
     5941 atagggaata ttataggtac atttgtcagc ccattttcac cagccttatg gttggcaatt
     6001 ggtttagcag aggcaaacat gggcacgtat attaagtatg cattcttttg gatttgggga
     6061 ttcgctatcg ttatgttagt aattgcaatg ttgatgggca ttgtgacgat ttaagtatga
     6121 aaaaatagaa actatggtca cgttgcaaaa tgaaataata gttgcataaa catgtcgaaa
     6181 tgacggacga atctttaaac aattttaaaa attaatgaaa taattgtgta gaaatatgaa
     6241 tttcactaaa tgttaataac tttgtgacgt tttagttaac agactaataa aaatttgaaa
     6301 atactatata tagtggtata acgtaatgag tagacacaat atataggaag aaggggtaaa
     6361 atgaatcaaa tcgaagaagc attaacgggt ttgatttcta aagatcctgc tattgttaac
     6421 gaaaatgcta acaaagatag tgatacattt tcaacaatga gagatttaac agcaggtatc
     6481 gtttctaaat cttacgcatt aaatcattta ttaccaaagc acgttgcaga tgcacatcaa
     6541 agaggggaca tacattttca cgacttagat tatcatccat tccaaccgtt aactaactgt
     6601 tgtttaatag atgctaaaaa tatgctacat aatggatttg aaataggcaa cgcgaatgta
     6661 acttcaccaa aatcaataca aactgcatca gcgcagcttg tacaaattat agccaatgtt
     6721 tctagcagtc aatatggtgg ctgtacggtt gaccgcgttg acgaattact tagtacatat
     6781 gcacgacata atgaagaaca acataggaat atcgcaaagc aatttgtcaa agaatctgaa
     6841 attgatcgtt atgttgatca acaagtcact aaagacatca atgatgcgat tgaaagttta
     6901 gaatatgaaa ttaatacctt atatacatct aatggacaga caccttttgt aacattagga
     6961 ttcggcttag gtacagatca tttaagtcgc aaaattcaac aagctatctt aaatactcgt
     7021 atcaaaggct taggaaaaga ccgcacgaca gcgattttcc cgaaacttgt attttcaatt
     7081 aaaaaaggaa ccaactttag tccgcaagat ccgaactatg acattaaaca actagcatta
     7141 aagtgttcaa cgaaacgtat gtatccagat attttaaatt atgacaaact cgtagaaata
     7201 ttaggtgatt tcaaagcgcc aatgggttgt cgttcatttt taccaagttg gaaagatgcg
     7261 gaaggtcatt ttgaaaataa tggtcgttgt aatcttggtg ttgttacact taatttacct
     7321 agaatggcat tagaatctgc cggtaatatg acgaaattct gggaaatctt ttatgaacgt
     7381 atcgatgtgt tacatgatgc attactttat cgtataaatc gtttgaaaga tgctgtaccg
     7441 aataacgcac cgattttata taaaagtggc gcatttaact ataaattaaa agaaacagat
     7501 gatgttgctg agttatttaa aaataaacgt gcaacgattt caatgggcta tatagggttg
     7561 tatgaaacag ctactgtttt ctatggtcca gactgggaaa catctcaaga agcaaaagca
     7621 tttacgcttg aaattcttaa agaaatgaaa cgttatcaaa cgaaatggac agaattatat
     7681 gacatttggt tcagtattta cagtacgccg agtgaatcgc taacggatcg tttttgtcgt
     7741 ttagaccaag agagatttgg agatattaaa gacattacag ataaaggata ttatcaaaac
     7801 tctttccatt atgatgtacg taaagatgtt acaccttttg aaaagttaga ttttgaaaaa
     7861 gattatcctt attatgcgag tggtggtttc attcactatt gtgagtatcc gaaattgcaa
     7921 cacaatttga aagcactaga agcggtatgg gactactctt atgacaaagt tggttactta
     7981 ggtacaaata ttccgattga tcattgttat gaatgtgatt acgatggaga ttttgaagca
     8041 actgaaaaag gatttaaatg cccgaactgt ggcaatgata atcctaaaac agttgatgtc
     8101 gttaaacgaa catgtggtta cctaggcaat ccagttcaac gtccagtaat taaaggccgt
     8161 cataaagaaa tttgcgcacg agtaaaacat atgaaagcgc ctaaagaatg atacttttag
     8221 acattaaaca aggacaaggt tatattgcta aaatagaatc aaatagcttt gttgacggtg
     8281 aaggagtaag atgcagtgtt tatgtatcag gatgtccatt taattgtgtt ggatgttata
     8341 acaaagcctc acaaaagttc agatatggcg agaaatacac tgatgaaata ttagcagaaa
     8401 tattagatga ttgcgatcat gattatatat ctgggctaag tctattaggt ggcgaaccat
     8461 tttgtaattt ggatattaca ttaaatcttg tcaaagcatt tcgagcacgt tttggaaata
     8521 caaagacaat ttgggtatgg actggatttt tatatgaata tttagcaaat gattgtacag
     8581 aacgtcgaga gttattatca tacattgacg ttttagtaga tggtctattt atacaacact
     8641 tattcaaacc tgatttacca tataaaggtt ctttaaatca acgcattata gatgtacaac
     8701 aatcactctc gcatgcgcgt atgattgaat atatagttag ttgaatatgt attagaagtc
     8761 aaggtaacat tcgttgcctt ggcttctttt taggttaggt acataattga aagttaataa
     8821 aagcaattct ttataaaaat atattgatag aatatgacct aacaatcatt ttgataccaa
     8881 tactaaaagt tgcatatccg ttttttaaaa aagttgaaag agaaaagtgg tattttagtg
     8941 ggaaggaagt ctaacttttt ggtagcgttt tacaataaat aaatattcgt taataacgta
     9001 taaatattct taaatgccat tctagtaaaa tttgttaaat tcgttaaatc gtaacttaac
     9061 actgttattt tagcgctatt aaggttttgt ttattacggg aaaaattata taaatattca
     9121 ataattgcca agtttcaaat tgtatgaaat ttgcattatt attaaatgtt agttattgtc
     9181 aattttgtga atcaatataa ttattacatt ttgagataaa tcgaaacagg attcataaaa
     9241 ttaataatta gggggagcac aattgaaaaa agagaaagtt atggactgga cgacctttat
     9301 agggacagta gctgtacttc tttttgcagt tatacctatg atggcttttc caaaagcaag
     9361 tgaagatatc atcactggta ttaatagtgc catttctgat tcaattggtt cgatatattt
     9421 atttatgggg ctggcgattt tttgctttgt aatgtatatc gcatttggta aatatggtaa
     9481 tgtcacactt ggtaaagcaa gtgataaacc agaatttaat acatttacat gggcggcaat
     9541 gctgttttgt gcaggcatag gctctgatat tttatactgg ggcgttattg aatgggcttt
     9601 ttactatcaa gttccaccaa atggcgcgaa aagtatgagt gatgaagcac tccaatatgc
     9661 gacgcaatat ggtatgttcc actgggggcc aattgcttgg gctatttatg ttctaccagc
     9721 attaccaatt ggttatttag tatttgttaa aaaacaaccg gtgtataaaa ttagtcaagc
     9781 ttgtcgtccg attttaaaag gtcaaacaga taaatttgta ggtaaagttg tagatatctt
     9841 atttatcttt ggattgctag gtggtgcggc aacatcacta gcgttaggtg tgccattaat
     9901 ttctgcaggc atagaaagat taactggttt agatggtaaa aatatgattt tacgttcggc
     9961 cattttatta acaatcacgg ttatatttgc cattagttca tatacaggat tgaaaaaagg
    10021 tattcaaaag ttaagtgata tcaacgtttg gctatccttt gtacttttag cctttatatt
    10081 tattattgga ccgactgttt ttattatgga aacgacagtg acagggttcg gaaatatgtt
    10141 gagagatttc tttcatatgg caacatggtt agaaccattc ggtggtatta aaggtcgaaa
    10201 agaaacgaat ttcccacaag actggacaat attctactgg tcatggtggt tagtatatgc
    10261 gccatttatc ggtttattta tcgctagaat ttcaaaaggt cgacgcctta aagaagtcgt
    10321 gctaggaaca attatttatg gaacgcttgg atgcgtatta ttctttggta tttttggtaa
    10381 ctatgctgtg tatttacaaa tttctggaca gtttaatgta acacaatatt taaatacaca
    10441 tggtacagag gcaaccatta ttgaagtggt gcatcattta ccattcccat cattgatgat
    10501 tgtactattc ttagtatctg ctttcttatt cttagcaaca acatttgatt cgggttcata
    10561 tattttagcg gcagcatctc agaaaaaagt ggtaggcgaa ccattacgtg ccaatcgttt
    10621 attctgggca tttgcattgt gcttattgcc attttcattg atgctagttg gtggtgaacg
    10681 tgcattagaa gtattgaaaa ctgcttcaat actggcaagt gtgccattaa ttgttatttt
    10741 tattttcatg atgatatcat ttttaatcat tttagggcgc gatagaatta aacttgaaac
    10801 gcgtgctgaa aaattaaaag aagttgaacg tcgttcattg cgaatcgttc aagtatcaga
    10861 agaagaacaa gacgataatt tataattcaa agcgggtctg ggacgacgaa atgaattttg
    10921 tgaaaatatc atttctgttc cattcccctt tttttagtag cattgtagga tgaactttta
    10981 ggttttcatt aatgttgtac taaaagattt aattttttag tgctccaagt acttatttat
    11041 tgtatgaagc atattctaaa tcgaagtttg aaagactctc attgattatt aaattaaata
    11101 aagggtactg cgtatgtaca attcaaatta atcgaaggat gaaataaaat gactaatcaa
    11161 tttaaaaata aacagtccaa attacatgac agtttagaat ccatcacaaa aaacttatat
    11221 gcgacaccta cttcggaact gccttttgat aatcgatttc tatttaaatc ttttatttta
    11281 aaacgtgaaa caggtaatat tgttatctat cattccggac acttaggtga ctcccaacaa
    11341 gacattgcat cattaggtgg tgtttcaaaa gtattgatga atcatgatca tgaatctata
    11401 ggaggttcta atcaagttga agccccttac tttatacatg aaaatgatgt ggctgcactg
    11461 aaacataaga tttctgttca aaaacaattt agtaatcgtg taatgttgga taaggattta
    11521 gaagttattc ccgcgcctgg acatacacca gggacgacac tatttttatg ggatgatggt
    11581 catcaccgtt acttatttac tggagatttt atatgttttg aagggaagag atggcgtaca
    11641 gttatattag gttcaagtga tagagaaaaa tctattcaaa gtttagagat ggttaaagaa
    11701 ttagattttg atgtacttgt accttgggtt actatcaaag atgaaccgtt agtttatttt
    11761 gtagaaaatg aatatgaaaa acgtgaacaa atacaaaata ttattgatag agtacgtgag
    11821 ggcgagaata gctaattgaa atatattggc gaagcaatgt aacgaatcta agaaagccct
    11881 agaaaatacc tccataattg attgtcatat aaaacaaaaa cggtaatttc tatttattga
    11941 gatagaaatt accgtttatt tcgtggacct attgcattgt ttttatcatg cataatcatc
    12001 attgtcgttg tttgagtcaa ttttaatttt cagaatcaga aggctgttct ggaattggga
    12061 aatatttgaa aatttcaccg ctttcaatcg cttcggttaa ctgttctaac cattcgtaat
    12121 aaacatgtgt atgatcaagc tgagatttaa ttttttgtgc ctcttgtgtt tcagcttcag
    12181 ttaaatcact gctttcaagt aatgaattga taatagcttg agcatctttt actgcttcga
    12241 cattgatgtc aatttcacgc tggaattttt tagtgaaaaa gtttcggaaa aagatgaaaa
    12301 agtctttctc ggcgataaaa tgttgtttgc ggcttcctct cgtaaattgt tgtttaacaa
    12361 tatcaaattc ctgcaatttc ttaacgccag cactcatact tggtttgctc atttgcaatt
    12421 gatgacgcat ttcatcaagc gtcatactgc cttcaaacac cattgtgcca tataagtttc
    12481 ctacacttct attagtgcca tacaaatcca ttgtctgtcc aattgaatta attacaatat
    12541 cttttgcttg ttctaattgt tgctgtttgt tctgagaacg agtcatcatt gcacctccgt
    12601 acatcatttt ggtcacgtta aaataaatac taatacatta taaaaccttt tctaaaaaaa
    12661 gacattaaaa atatttaaag cattaaagtt aaatgtttcg ttaaataaaa atctaacgaa
    12721 cttacaaaac ttaattcttg agttgttttg taaattgaca catttttcat ttctatgcta
    12781 acataagttt gtaaaattcg ttaaataaaa atttaacaaa cttaacggag gttgttgaat
    12841 ggaactttta aaacatttat ctcagcgtca atatattgat ggtgagtggg ttgaaagcgc
    12901 gaataaaaat acaagagata ttatcaatcc ttacaatcaa gaagtgatat ttacggtttc
    12961 tgaagggaca aaagaggatg cagaacgtgc aatcttagct gcaagacgtg cgtttgagtc
    13021 tggtgaatgg tcgcaagaaa ctgctgaaac aagaggtaaa aaagtacgtg ccatcgcgga
    13081 caagattaaa gaacatcgcg aagcgttagc acgattagaa acattagata ctggaaaaac
    13141 gttagaagaa tcatatgcag atatggatga tattcataat gtgtttatgt attttgctgg
    13201 attagcagat aaagacggtg gcgaaatgat tgattcacca attccagata cagaaagcaa
    13261 aattgttaaa gaaccagtag gtgtagttac acaaattaca ccttggaatt atccgttatt
    13321 acaagcatca tggaaaattg cgccagcgct tgctacgggt tgttcactag ttatgaaacc
    13381 aagtgaaatt acaccattaa caacaatacg tgtttttgaa ttaatggaag aagttggttt
    13441 ccctaaagga acaattaatc ttattctagg tgcaggttct gaagttggtg acgtaatgtc
    13501 aggtcataaa gaggttgacc ttgtatcatt tacaggtggc attgagactg gtaagcatat
    13561 tatgaaaaat gctgctaata atgttacgaa tattgccttg gaacttggcg gtaaaaatcc
    13621 aaacattatc tttgatgatg ctgattttga attggcagta gaccaagcgt taaatggtgg
    13681 atatttccat gcaggtcaag tttgttcagc aggatcaaga atattagtac aaaacagtat
    13741 taaagacaaa tttgagcaag cacttattga tcgcgtgaaa aaaatcaaat taggtaatgg
    13801 ttttgatgct gatactgaaa tgggaccagt gatttcaaca gaacatcgta ataagatcga
    13861 atcttatatg gatgtagcta aagcagaagg cgcaacaatt gctgttggtg gtaaacgtcc
    13921 agatagagat gatttaaaag atggtctatt cttcgagcca acagtcatta caaattgtga
    13981 tacgtcaatg cgtattgtac aagaagaggt tttcggacct gtcgttactg tagaaggctt
    14041 tgaaactgaa caagaagcga ttcaattagc gaatgattct atatatggtt tagcaggtgc
    14101 tgtattttct aaagatattg gaaaagcaca acgcgttgct aacaagttga aacttggaac
    14161 ggtgtggatt aatgatttcc atccatattt tgcacaagcg ccatggggtg gatacaaaca
    14221 atcaggtatc ggtagagaat taggcaaaga aggcttagaa gagtaccttg tttcaaaaca
    14281 cattttaaca aatacaaatc cacaattagt gaattggttt agcaaataaa aattagataa
    14341 ggtgagtgcc attgtaagaa cacaagacac tcactttgtt ttgtataagt ggcgaaatgt
    14401 tgattgataa tttggactaa acgcaaaatg aatcatagat tatttcatta ctgttagtaa
    14461 caatcgtaaa aggaaaagcg agtgttttgg ttagctaagt ttagcaattc aacgataacc
    14521 aatcagccac taacaaatat ttcatgcaat actcactttg aaatacaaca aactttggag
    14581 gtcataacga tgagtaacaa aaacaaatca tatgattatg tcatcattgg aggaggcagt
    14641 gcaggttctg tactaggtaa tcgtctgagt gaagataaag ataaagaagt cttagtatta
    14701 gaagcgggtc gcagtgatta tttttgggat ttatttatcc aaatgcctgc tgcgttaatg
    14761 ttcccttcag gcaataaatt ttacgattgg atttattcaa cagatgaaga accacatatg
    14821 ggcggtcgta aagtagcaca tgcccgaggt aaagttttag gtggatcaag ttcgattaat
    14881 ggcatgattt atcaacgtgg taatccaatg gactatgaag gctgggcaga accagaaggt
    14941 atggaaactt gggattttgc gcactgttta ccgtatttta aaaaattaga aaaaacatac
    15001 ggtgcagcgc cttatgataa atttagaggc catgatggac caattaagtt aaaacgaggg
    15061 ccagcaacga atcctttatt ccagtcattc tttgatgcag gtgttgaagc aggctatcat
    15121 aaaacacctg atgtgaatgg atttagacaa gaaggttttg gaccgttcga tagtcaagta
    15181 catcgtggtc gccgaatgtc agcttcaaga gcatatttac atccagcgat gaagcgtaaa
    15241 aacttaaccg ttgaaacacg tgcctttgta actgaaattc attatgaagg tagaagagca
    15301 actggtgtta cgtataagaa aaatggcaaa ctacatacca tcgatgctaa tgaagtcatt
    15361 ttgtctggtg gggcattcaa tacgccacaa ttactacaat tatctggtat cggtgattca
    15421 gagttcctaa aatcaaaagg cattgagcca cgtgttcatt tacctggtgt gggtgaaaac
    15481 tttgaagatc acttagaggt atacattcaa cataaatgta aggaacctgt atcattacag
    15541 ccaagcttag atatcaagcg catgccattt attggtttac aatggatttt cacacgtaca
    15601 ggtgcagcag catctaacca ttttgaaggt ggcggatttg tacgttcaaa taacgaagtt
    15661 gattatccta acttaatgtt ccatttctta ccaatagctg taagatatga tggtcaaaag
    15721 gcagcggtcg cacatggtta tcaagttcac gtaggaccaa tgtattcaaa ctctcgtggt
    15781 agcttgaaaa tcaaatctaa agatccattc gaaaaaccaa gtatccgctt taattattta
    15841 tcaacagaag aagataaaaa agaatgggta gaagcaattc gtgtagcaag aaatatctta
    15901 tctcaaaaag caatggatcc attcaatggt ggagaaattt caccaggacc agaagtgcaa
    15961 acagacgaag aaattcttga ttgggttcgt agagatggtg aaacagcatt acacccatct
    16021 tgtagtgcta aaatgggtcc tgcttcagat ccaatggcag tagttgaccc gttaacaatg
    16081 aaagtccacg gtatggaaaa tttacgtgta gttgatgcat cagctatgcc acgtacgaca
    16141 aatggcaata tacacgcacc tgttttaatg ttagctgaaa aagcagcaga cattatacgt
    16201 ggcagaaagc ctttagagcc acaatatatt gattattata agcatggtgt tcatgatgaa
    16261 aatgaaggtg caatcgaagt aaaaccatac gctaaataaa tttatatagt agactcttag
    16321 tctggcatct attgtattgg tgccaggctt ttttgcgttt tatctaaatg agaagttaat
    16381 gtgttgttca aaaacggtgt gtgagtatat gaactgaaaa tagagttaat tgtacattgt
    16441 gatgattaat tgacctaatt ggcagacatt aaaagagtgc acaaccttaa aaagttcact
    16501 aaatagacat ttaattgtgt tttcttgaaa gctttcgtgg taggatttat ataagtaaat
    16561 tgagagaagg aatactatga gcttttacga atatatacaa acatttaaag atgataaaac
    16621 accattaggc gaattagcga tttggattaa agaagatgat tcattcccaa aacaagagaa
    16681 gctaacagaa aacattttgt cttattttca tcaaatgtcc aatatagatc atgagttttt
    16741 agaaattgta aaaagatcac tttctctgta tgatcaatta aaatcgtaaa atcagaaata
    16801 aagttagcta aaataaaagg ccggtgattt cacagatgaa gttgtcggct attttcatga
    16861 ctaatgttaa ataatgatca tgaaattgaa gacacaaaaa tagtgggcga ctcctgtccc
    16921 ccatttgcat gaaatattaa tactttttac aaagtcttat cttttaaaaa gaagccaaac
    16981 tatgattgta attaataaat acgggaatga attagtaata gatataaagt aatatgacca
    17041 aaaatataaa tgaaatgaag gcatttatta tgaaaattta tgatgaatat agaagttatg
    17101 taatagaaga actggatgat tgtctcacaa tacaaaaaaa taatgacact gcatattacg
    17161 atgtgctaga agcgataaat gatttatcaa atgattcatt atgtgtgtta aatcatttat
    17221 atattaacga aggacaagaa gaaacattcg agcaaaaatt tttgcgacgt aataagcatt
    17281 taaaaaacgt tgatggattt aaagcattaa ggtttttaag acctagaaca gctggtagac
    17341 actatatcat tattacgctg tgggaaaata ggcaagcatt ttatcattgg caaaattcag
    17401 ctgagtacaa gcatacacac aagcatcgtg gcacttcgaa aggtgcagat gtaaaaataa
    17461 ttaatcgtga attatcatac aatattcgta ttgaattagc tgatatggtc tagcccgaga
    17521 tatcaaccct caatcatttg aaagtccttg aatgctacgt catgtcaaag tgtagtgtcg
    17581 aggactttgg tatttaaccc tatttaattg aaaattctgt aaagataggc gtatgatgta
    17641 tataattcat ttataaaatt acaatgttca tcacaggaaa agtggatgta tgaaaggaag
    17701 ttagttatgc aaaagtcagc tctattcgcg cctgaaaaat ataacattat ttcggaaatc
    17761 gaaaaatata gccacatacc agataaaaag gcaattttat atcataatac tgaaggcgaa
    17821 gatatatcag tcacttacca acagctcatt gaacagtcta ataaagtagg taatgtttta
    17881 gcatcgcacg gtttatctaa aggcgataaa gtactcatta tgatgccacg atctattgcg
    17941 acgtatgaat tatatattgc tgctttaaaa ttaggtgttg caatcattcc atgttctgaa
    18001 atgctaagaa ctaaagattt acaataccgt atcacacatg gtgaaattaa tgcagttatt
    18061 gcactagagc cattcacagt tgaatttgaa aaaattaaag aatatgatgc gttaactaaa
    18121 tttgttattg ccggtcaaaa agatggatgg atttcacttg aagctgaaaa agaaaacgca
    18181 agtaacaaac tagaaatggc agatacgacg cgtgatgata tggcgattct gtcatataca
    18241 tctggtacaa ctggcaatcc taaagccgtt acacattgtc atggatgggg atttgcacat
    18301 ttacaaatgg caccaaaaca ttggttatgt attaatgaag atgacttagt ttgggcaaca
    18361 gcagcaccag gttggcaaaa atgggtatgg agtcctttct tatctgtatt aggttcaggt
    18421 gcaactgctt ttgtatttaa tggaaggttc agtcctgaaa catacttaga attgcttcaa
    18481 aaatatcaaa ttaatgtttt atgttgtaca ccgacagaat atcgtatgat ggctaaatta
    18541 cagaacttaa atgattatga cttaacgcat ttacatagtg ccgtttctgc aggtgagcca
    18601 ctaaatcgag aagtagttga acaattcaag aaatacttta atttaactgt aagagatgga
    18661 tatggccaga ctgaaagtac cttgcttata ggctttttaa aagatacacc acagaggatt
    18721 ggatcaatgg gtaaaggcat tccgggtagt tcggtcactg tagtagatga tgaggggaat
    18781 agcgtacctg ctaatacgaa agggaatatt gcagtaccac ttgatttgcc agcacttttc
    18841 aaagggtatt ataaagagcc agaacgtacg gcgaaagcgc aaacaggtaa atactacatc
    18901 actggtgact tagctcatgt agatgatgat ggttatttct ggtttgaagg acgtcgagat
    18961 gacattatta ttagttcagg ctatacgatt gggccttttg aagttgaaga tgcgttaacg
    19021 aatcatccag ccgttaaaga atgtgctgta gtagcaaaac ctcatgatat acgaggaaat
    19081 attgttaaag cattcgttat tttacaggac catactgcgg gtgatgatac gttagttaaa
    19141 gaactacaac aatttgttaa aaatgaagtt gcaccatata aatatccgag agagattgaa
    19201 ttcgttgacg acttaccaaa aacaaactca ggtaaaattc gtcgtgttga actacgagat
    19261 gctgaaatag aaaaatggca acaacaaaaa gatagcaatc aataaacata gcaaagcagt
    19321 ccaatgcgta aggtatgatg atacgcactg gactgcttgt ttatttatga tgttctttgt
    19381 cgtctagtta taaatattaa aattagttta gaaacaagcg caaccagtat taagaatgag
    19441 aaaatataag atgttaaacc tcgaatatcc cagtttaata aaaacacatt actatcaccg
    19501 gcattaagtt gaaactcagt atgtgttaga acgataatta caatacctat aagtgcaaga
    19561 ttcaaaataa ttttcaatat taaaggtaat tgtaatgttt ttcttggatg cagatattga
    19621 atatcctttt tatgattgat tttacactta tattgtatca aaatagagta taggtttaaa
    19681 atgctataag ccaaatgtag attaaaacaa tcgttagcta tttgttgtca aataat
//
LOCUS       DALAGD010000057        19695 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.057, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000057 DALAGD010000000
VERSION     DALAGD010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19695
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.057"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(122..2098)
                     /gene="sfaD"
                     /locus_tag="O2701_001648"
                     /gene_synonym="sfnaD"
     CDS             complement(122..2098)
                     /gene="sfaD"
                     /locus_tag="O2701_001648"
                     /gene_synonym="sfnaD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine--citrate ligase SfaD"
                     /protein_id="HCZ4293539.1"
                     /translation="MNLNLIFKEQTLKFNKEEQETYLFLQQHNSDWANIFKEMILQGR
                     DKVTQRLVTSMHRENLVKARTQSKKILSRDLIMLDISTTHILEIQFPQAKQTLYAPIT
                     GEHAFDRIDVEGPFYIKDDITNTITRVHHPNEILECILIEAPDLKNAASDQFQQDLIN
                     SATNMTFAISYQALSMQHDSAPLFNIIENSEDSYLRSEQAVIEGHPLHPGAKLRKGLN
                     ALQTFLYSSEFNQPIKLKIVLIHSKLSRTMSLSKDYDTTVHQLFPDLIKQLENEFTPK
                     FNFNDYHIMIVHPWQLDDVLHSDYQAEVDKELIIEAKHTLDYYAGLSFRTLVPKYPAM
                     SPHIKLSTNVHITGEIRTLSEQTTHNGPLMTRILNDILEKDVIFKSYASTIIDEVAGI
                     HFYNEQDEADYQTERSEQLGTLFRKNIYQMIPQEVTPLIPSSLVATYPFNNESPIVTL
                     IKRYQSAASLSDFESSAKSWVETYSKALLGLVIPLVTKYGIALEAHLQNAIATFRKDG
                     LLDTMYIRDFEGLRIDKAQLNEMGYSTSHFHEKSRILTDSKTSVFNKAFYSTVQNHLG
                     ELILTISKASNDSNLERHMWYIVRDVLDNIFDQLVLSTHKSNQVNENRINEIKDTMFA
                     PFIDYKCVTTMRLEDEAHHYTYIKVNNPLYRENN"
     gene            2200..3393
                     /gene="sfaA"
                     /locus_tag="O2701_001649"
     CDS             2200..3393
                     /gene="sfaA"
                     /locus_tag="O2701_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A export MFS transporter"
                     /protein_id="HCZ4293540.1"
                     /translation="MTKYFFSSSFLLFLGNWIGQIGLNWFVLTTYHNAVYLGIVNFCR
                     LVPILLLSVWAGAIADKYDKGRLLRITISSSFLVTAILCVLTYSFTAIPISVIIIYAT
                     LRGILSAVETPLRQAILPDLSDKISTTQAVSFHSFIINICRSIGPAIAGVILAVYHAP
                     TTFLAQAICYFIAVLLCLPLHFKVTKIPEDASRYMPLKVIIDYFKLHMEGRQIFITSL
                     LIMATGFSYTTLLPVLTNKVFPGKSEIFGIAMTMCAIGGIIATLVLPKVLKYIGMVNM
                     YYLSSFLFGIALLGVVFHNIVIMFICITLIGLFSQWARTTNRVYFQNNVKDYERGKVL
                     SIIMMDRGMIPLGSLLMSICADVFGIVRTFSIMGISTICITMVFYFINRKLKLKLEES
                     NHGIS"
     gene            3380..5137
                     /gene="sfaB"
                     /locus_tag="O2701_001650"
     CDS             3380..5137
                     /gene="sfaB"
                     /locus_tag="O2701_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A synthetase SfaB"
                     /protein_id="HCZ4293541.1"
                     /translation="MVYLEWAKADRNIQYRVINAIIKERIYPEQTFISQKGSLIEIQY
                     HMHVLTIEVVRKSALERYEFTGDITYLNKGETSLIITLEGLLDVLNHDFDIPISERLR
                     EELIHSRDSLVETYKQMSHRQTLISQSFKFSRLPQDINFFSWLQHVKDSDKTDDLTYS
                     ESLVPEGHPTHPLTKTKLPLTMEEVRAYAPEFEKEIPLQIMMIEKDHVVCTAMDGNDQ
                     FIIDEIIPEYYNQIRVFLKSLGLKSEDYRAILVHPWQYDHTIGKYFEAWIAKKILIPT
                     PFTILSKATLSFRTMSLIDKPYHVKLPVDAQATSAVRTVSTVTTVDGPKLSYALQNML
                     NQYPGFKVAMEPFGEYANVDKDRARQLACIIRQKPEIDGKGATVVSASLVNKNPIDQK
                     VIVDSYLEWLNQGITKESITTFIERYAQALIPPLIAFIQNYGIALEAHMQNTVVNLGP
                     HFDIQFLVRDLGGSRIDLETLQHRVSDIKITNDSLIADSIDAVIAKFQHAVIQNQMAE
                     LIHHFNQYDCVEETELFNIVQQVVAHAINPTLPHANELKDILFGPTITVKALLNMRME
                     NKVKQYLNIELDNPIKKEV"
     gene            5144..6214
                     /gene="sfaC"
                     /locus_tag="O2701_001651"
     CDS             5144..6214
                     /gene="sfaC"
                     /locus_tag="O2701_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A biosynthesis protein SfaC"
                     /protein_id="HCZ4293542.1"
                     /translation="MAHVNINISKIKYNAKVLQTVFQSKNMQFTPVIKCIAGDRTIVE
                     SLKALGINHVAESRLDNIISIADQDLTYTLLRTPAKKEISDMIEKVDMSIQTELSTIH
                     QINEVAEVLGKKHKILLMVDWKDGREGVLTYDVLDYIKEIIHLKNIHFVGLAFNFMCF
                     KSDAPSDDDIFMINRFVSAVEREIGYRLKIISGGNSSMLPQLLYNDLGKINELRIGET
                     LFRGVDTTTNQAIAMLYQDAITLEAEILEIKPRVNTQTHESFLQAIVDIGYLDTKVDN
                     ISPMDQHINILGASSDHLMLDLNGQGHYQVGDHISFSLNYEALSHSMYMKNLHKVYID
                     DSKIDTLLQNFDVKSPAMVNHY"
     gene            complement(6315..6440)
                     /locus_tag="O2701_001652"
     CDS             complement(6315..6440)
                     /locus_tag="O2701_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293543.1"
                     /translation="MYHCYIIFALIQLTPFSMTVIPVIQLILSVAILCDELLHLI"
     gene            6606..7589
                     /locus_tag="O2701_001653"
     CDS             6606..7589
                     /locus_tag="O2701_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCZ4293544.1"
                     /translation="MRGLKTFSILGLIVALLLVAACGNTDNSSKKESSTKDTISVKDE
                     NGTVKVPKDAKRIVVLEYSFADALAALDVKPVGIADDGKKKRIIKPVREKIGDYTSVG
                     TRKQPNLEEISKLKPDLIIADSSRHKGINKELNKIAPTLSLKSFDGDYKQNINSFKTI
                     AKALNKEKEGEKRLAEHDKLINKYKDEIKFDRNQKVLPAVVAKAGLLAHPNYSYVGQF
                     LNELGFKNALSDDVTKGLSKYLKGPYLQLDTEHLADLNPERMIIMTDHAKKDSAEFKK
                     LQEDATWKKLNAVKNNRVDIVDRDVWARSRGLISSEEMAKELVELSKKEQK"
     gene            7601..8632
                     /locus_tag="O2701_001654"
     CDS             7601..8632
                     /locus_tag="O2701_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCZ4293545.1"
                     /translation="MAIKEISSQSAIDHKRKRRTTLTYIVSLCFLFICIYLNMAIGSS
                     KINFSDIIHYVTGHTDTKATFLLHNVRMPRMIAGLFIGGALAVSGLLMQAMTRNPLAS
                     PKIFGVSSGASFFIVFVTIIIPALEYYALYLGVIGAFIGGLTVYTLSGATKGMTPIKL
                     ALAGMAIHLFFSSMTEGIIILNENSNEQVMFWLVGSLSSMKWDEILTILPWIIGALIV
                     TIFIGRQLTIMELGDDIAKGLGQNINKVRIIIGLLVIILTGMSVSVAGPIGFVGLIVP
                     HIVKRYVSKNYLVMIPLTFIIGADLLLLSDVLSRLITYPYESPVGIVTSFVGALYFLF
                     ITIKGVKRI"
     gene            8629..9597
                     /locus_tag="O2701_001655"
     CDS             8629..9597
                     /locus_tag="O2701_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCZ4293546.1"
                     /translation="MMTTKIIRRYSLITILLIVSIFISLCVGSVMIHPIDAIKGIFTQ
                     DDFILNEYRIPRTLLGIIIGSSLAISGAIIQAVIRNPLASPDVIGISKGASLAAVIII
                     MTFPTAPLFVLPIGSFLGAFAVSLFLSFLISKYDVKGSKLALIGLAIGAICTAIVQFL
                     LIRNPLEANKALVWLTGSLYGHDMSNIYTILPWFLITIPIIILLSYQLDVLNLGDAVA
                     TALGLKVKTIKMLLLILAVILAGSAISVVGGISFLGLIAPHIARQLIGNKTLHVIIMS
                     GLIGAILLTFGDGLARGIHPPLDIPVGVIIAIIGAPYFLILLRRMK"
     gene            9833..11203
                     /locus_tag="O2701_001656"
     CDS             9833..11203
                     /locus_tag="O2701_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCZ4293547.1"
                     /translation="MDSFSRKEIVIGRLKFITMSLIGILLFLVPIPVEQDGQKQTTLP
                     VAFLAGVLKDVLGGVMPFLIVTIITLSGIITLICSTILKDKLKPDGLMNNAFNVRIGW
                     LILRILAVVFAWMTFLRIGSKVIYSDETGGLLFSSLLPTLVAVFLFAALFLPLLMEYG
                     LLEMLGPIFRPVMRPLFTLPGRSTVDNLASFIGDGTVGVLITSRQYGEGYYSRREATV
                     ISTTFSVVSITFAIVVAETVHMQNQFFAFYLSVIVSCLVAAVIMPRIWPLNKIPDEYA
                     KEVPESARTEALPEGKTALRHGFDTATEVGIKAPGVIDFFKSGLKTVIDMWFVILPVV
                     MSIGTIATIIANYTPFFVILGKPFVPFLELMQIPEAAQASQTIIIGFADMFLPSILIE
                     GVQNDITRFVIGALSISQLIYLSEVGGVILGSKIPVSIGKLFMIFLIRTIITLPIISL
                     MAHLLL"
     gene            complement(11335..11595)
                     /locus_tag="O2701_001657"
     CDS             complement(11335..11595)
                     /locus_tag="O2701_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293548.1"
                     /translation="MAMTVKKDNNEVRIQWRVADIKIPTSEIKNITQDQDIHAVPKLD
                     SKDVSRIGSTFGKTNRVIIDTEDHEYIIYTQNDQKVYNELTK"
     gene            11772..12287
                     /locus_tag="O2701_001658"
     CDS             11772..12287
                     /locus_tag="O2701_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCZ4293549.1"
                     /translation="MTGKTHASCGMLVGALTTQYFQTDIFSSVTVIILATLASLLPDI
                     CHTQSKIGRRFKVISFFVRLIFGHRTFTHSILFIAIIAFLLQIIQTPNYYMAAIIIGL
                     VSHVILDMITPKGVKLFYPLPFNVKTPIQFKTGGLVDLSLATALMVGTVYVLFQPFIN
                     DIISNWNTKFL"
     gene            12331..13518
                     /locus_tag="O2701_001659"
     CDS             12331..13518
                     /locus_tag="O2701_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HCZ4293550.1"
                     /translation="MLDKNQLAKYKQDHLCEYEKIMSNNEKEALEEKVASLDLDFIAK
                     LYNDLYINKKTIDDVSAVSEVKYDIKSQMSDDEIKRLEEQGLQAIKEGQFAVLLMAGG
                     QGTRLGYKGPKGSFEIEGVSLFELQANQLKTLNHQSGHTIQWYIMTSDINHEETLAYF
                     EAHSYFGYDQEAIHFFKQDNIVALSEEGKLILNQQGRIMETPNGNGGVFKSLDKAGYL
                     EEMSNNGVKYIFLNNIDNVLVRVLDPLFAGFTVEHDYDITSKTIQPKPGESVGRLVNV
                     DCKDTVLEYSELDPEVANQFNNANIGIHAFKLGFILNAVNRELPYHLAIKNLKQLDEN
                     FGVIEQPTLKFELFYFDIFTYGTSFVTLQVPREEEFSPLKNKEGKDSVATATEDLRRM
                     GLI"
     gene            13537..14220
                     /locus_tag="O2701_001660"
     CDS             13537..14220
                     /locus_tag="O2701_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCZ4293551.1"
                     /translation="MSKSTKKSNDTIVETFKDIIPLSFGEEIGNAASHGVAALLTLLV
                     LPYAAVHSYIAHGTLASVSMSIYVISIFMMFISSTIYHSMQNETPHKYILRIIDHSMI
                     YVAISGTYTPILLTVVGGWIGWTVFILLWGTTLWGILYKSIAVKVNQKLSLIVYLVMG
                     WVGIILLPIIFIRTSWIFILFIFLGGVSYTIGAWFYAQKNRPYFHMIWHIFIVIASLL
                     HLIAILYFM"
     gene            14397..15740
                     /locus_tag="O2701_001661"
     CDS             14397..15740
                     /locus_tag="O2701_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ4293552.1"
                     /translation="MRLKSIITVIALILIMFMSAIESSIISLALPTIKQDLNAGNLIS
                     LIFTAYFIALVIANPIVGELLSRFKIIYVAIAGLLLFSIGSFMCGLSTNFTMLIISRV
                     IQGFGSGVLMSLSQIVPKLAFEIPLRYKIMGIVGSVWGISSIIGPLLGGGILEFATWH
                     WLFYINIPIAIIAIILVIWTFHFPEEETVAKSKFDTKGLTLFYVFIGLIMFALLNQQL
                     LLLNFLSFILAIVVAMCLFKVEKHVSSPFLPVVEFNRSITLVFITDLLTAICLMGFNL
                     YIPVYLQEQLGLSPLQSGLVIFPLSVAWITLNFNLHRIEAKLSRKVIYLLSFTLLLVS
                     SIIISFGIKLPVLIAFVLILAGLSFGYIYTKDSVIVQEETSPLQMKKMMSFYGLTKNL
                     GASIGSTIMGYLYAIQSGIFGPNLHNVLSAVAVISIGLIVLWVVFFKEQSSQSKE"
     gene            15839..16312
                     /gene="sepA"
                     /locus_tag="O2701_001662"
     CDS             15839..16312
                     /gene="sepA"
                     /locus_tag="O2701_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter SepA"
                     /protein_id="HCZ4293553.1"
                     /translation="MIVNYLKHKFYNLLTTMIVLFIFVLSGAIFLTFLGFGLYGLSRI
                     LIYFRLGDFTYNRSMYDNLLYYGSYIIFGYFIIFAVEHLMDYFRKMLPENAYFRGATF
                     HLISYTVATTLFYFIIHLNYVYINIDFWVIMVIIGFLYVCKLQFYPESKNLNNRK"
     gene            16634..18076
                     /gene="lmrS"
                     /locus_tag="O2701_001663"
     CDS             16634..18076
                     /gene="lmrS"
                     /locus_tag="O2701_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter LmrS"
                     /protein_id="HCZ4293554.1"
                     /translation="MAKVELTTRRRNFIVAVMLISAFVAILNQTLLNTALPSIMRELN
                     INESTSQWLVTGFMLVNGVMIPLTAYLMDRIKTRPLYLAAMGTFLLGSIVAALAPNFG
                     VLMLARVIQAMGAGVLMPLMQFTLFTLFSKEHRGFAMGLAGLVIQFAPAIGPTATGLI
                     IDQASWRVPFIIILGIAILAFVFGLVSISSYNEVKYTKLDKRSVMYSTIGFGLMLYAF
                     SSAGDLGFTSPIVIGALILSMVIIYLFIRRQFNITNALLNLRVFKNRTFALCTISSMI
                     IMMSMVGPALLIPLYVQNSLSLSALLSGLVIMPGAIINGIMSVFTGKFYDKYGPRPLI
                     YTGFTILTITTIMLCFLHTDTSYTYLIVVYAIRMFSVSLLMMPINTTGINSLRNEEIS
                     HGTAIMNFGRVMAGSLGTALMVTLMSFGAKIFLSTSPSHLTATEIKQQSIAIGVDISF
                     AFVAVLVMAAYVIALFIREPKEIESNRRKF"
     gene            18224..19288
                     /locus_tag="O2701_001664"
     CDS             18224..19288
                     /locus_tag="O2701_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HCZ4293555.1"
                     /translation="MLTVDQVKELVGEIKDPIIDVPLKETEGIVEVSIKEEKEHVSVK
                     LAMAQLGGAPQLDLQMAVVNALKENGAKTVGIRFETLPEEKVNQFKPKEENKPKTIEG
                     LLSQNNPVEFIAIASGKGGVGKSTVAVNLAVALAREGKKVGLVDADIYGFSVPDMMGI
                     DEKPGIKGKEVIPVERHGVKVISMAFFVEENAPVIWRGPMLGKMLTNFFTEVKWGDIE
                     YLILDLPPGTGDVALDVHTMLPSSKEIIVTTPHPTAAFVAARAGAMAKHTDHSILGVI
                     ENMSYFESKETGNKEYVFGKGGGTKLADELNTQLLGELPLEQPSWNPKDFAPSIYQSD
                     DRLGKIYSSIAQKVIAATNK"
     gene            19377..19562
                     /locus_tag="O2701_001665"
     CDS             19377..19562
                     /locus_tag="O2701_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293556.1"
                     /translation="MLSNKRVEKEAGCEHKKFLKKLLTLNSKVDNMIKSSKTTQLFLK
                     KNFKSVKLTIEITIKTI"
BASE COUNT         6523 a         2678 c         3611 g         6883 t
ORIGIN      
        1 cctgtttttt gtgattttta ataatttgaa tatggaaaat gtattattct ctcatttgta
       61 tagattgtat ttaataagtt aatgtaatcc ttgagctcac gattaataaa attctataac
      121 cttaattatt ttctcgatac aaagggttat taactttaat ataagtataa tgatgtgcct
      181 catcttcaag acgcattgtt gtaacacact tataatctat aaatggcgcg aacatggtat
      241 ctttaatttc atttatgcga ttctcattta cttgattaga tttgtgtgtc gaaagtacaa
      301 gttgatcaaa aatgttatct agtacatcac gaacgatata ccacatatgt ctttctaagt
      361 ttgaatcatt tgatgcttta gagattgtaa gaattaattc gcctaaatgg ttttgaacgg
      421 tagaataaaa ggctttgtta aacacagacg tttttgaatc agtaagaatt cttgattttt
      481 catggaaatg agatgtactg tatcccattt catttagttg tgctttatca attcttaaac
      541 cttcaaagtc tctaatatac atcgtgtcca atagaccatc tttccgaaat gttgcaatag
      601 cattttgcaa gtgagcctct aatgcaatgc catatttagt aactagtggg attacgagac
      661 caagcaatgc tttactataa gtttcaaccc acgatttcgc cgatgattca aaatcagaca
      721 atgatgcagc tgattgataa cgtttaatca atgtcacgat aggtgattca ttgttaaatg
      781 ggtaggttgc aactaagctt gaaggaatca atggtgtgac ttcttgtgga atcatttggt
      841 atatattttt tctaaataat gtacctaatt gttcacttcg ttcagtttga tagtctgctt
      901 cgtcttgctc attataaaaa tgaataccag caacttcatc aataattgtt gatgcatagg
      961 acttaaatat gacatctttc tccaaaatat catttaaaat acgtgtcatt agtggaccat
     1021 tgtgcgtcgt ttgttctgat aatgtacgaa tctcacctgt aatatgaacg tttgtcgaca
     1081 atttgatgtg tggcgacata gctgggtatt taggaactaa tgttctgaaa gataaaccag
     1141 cataataatc caacgtatgt tttgcttcaa tgattaattc tttatctact tctgcttgat
     1201 aatcagaatg taatacgtca tctaattgcc atggatgaac aatcataatg tgataatcat
     1261 taaagttaaa ctttggcgta aattcatttt ctaattgttt aattaagtcc ggaaatagtt
     1321 gatgaacagt tgtatcataa tctttagaca gtgacatagt acggcttaat ttactgtgaa
     1381 tcagtactat tttcaactta ataggttgat tgaattctga agaatatagg aatgtttgta
     1441 atgcatttaa acctttacgt aatttagccc caggatgtag cggatgacct tcaataacgg
     1501 cttgctctga acgcaagtaa ctatcttcgc tattttcgat aatattaaat aaaggtgcag
     1561 aatcatgttg cattgacagt gcttgatagc taattgcaaa tgtcatatta gttgcactgt
     1621 ttattaaatc ttgctgaaat tgatcactag cagcattttt taaatctggt gcttcaatta
     1681 aaatacactc aagaatttca tttggatggt gtactcgtgt aatcgtattt gtaatgtcat
     1741 ctttaatgta gaaagggcct tcaacatcaa ttcgatcaaa ggcgtgttct ccagtgatag
     1801 gagcatataa tgtttgctta gcttgtggga actggatttc tagtatatga gtcgttgaga
     1861 tatctaacat aatcaaatca cgactcaata ttttcttact ttgagtgcgc gctttaacta
     1921 agttttcgcg atgcattgat gtgaccaatc tctgagtgac tttatctctt ccttgtaaaa
     1981 tcatctcttt aaaaatatta gcccaatcgc tattatgttg ttgtaaaaat aaatatgttt
     2041 cttgttcttc tttgttaaat tttaatgtct gttctttaaa aattaagttc aagttcataa
     2101 ttcacctcta tgaaatattt tacaaaagca agatagattt gtataatcca tattaatgat
     2161 aatgattctt attatcaaca gaatgcgggt gtaagtttta tgacaaaata tttttttagc
     2221 agttcttttc tactatttct aggtaattgg attggacaaa tagggctaaa ttggtttgta
     2281 cttaccactt atcataacgc agtttatctg gggattgtca atttttgcag acttgtacca
     2341 atattattac taagtgtgtg ggcaggggca attgccgata aatatgataa agggcgattg
     2401 ctgagaatta caatttcatc atcattttta gtaactgcaa ttttatgtgt gctcacgtat
     2461 agtttcactg caattccaat tagcgtcatt attatatatg cgacattaag agggatttta
     2521 agtgcggttg aaacaccttt aagacaagca atcttaccag atttatcaga taaaatatct
     2581 actacacaag ctgtatcatt tcattcattc atcattaata tttgtcgttc aatagggcct
     2641 gccattgctg gtgtcatatt agcagtctat catgcgccaa caacatttct tgcacaggca
     2701 atttgttatt ttatcgcagt tttattatgc ttaccattac attttaaagt aactaaaata
     2761 cctgaagatg catcaagata catgccgtta aaagttatta tagattactt caaattacat
     2821 atggaaggtc gacaaatatt tataacatca ttattgatta tggcgacagg tttttcatat
     2881 acgacacttt taccagtttt gacaaacaaa gtatttccgg ggaaatctga aatatttggt
     2941 atcgctatga cgatgtgtgc cattggtggt attattgcaa cgctagtttt acctaaagta
     3001 cttaaatata ttggtatggt aaatatgtat tatttaagtt catttttatt tggcattgct
     3061 ttgttaggtg tggtatttca caatattgtc atcatgttca tttgtattac attgattggg
     3121 ttatttagtc aatgggcacg tacgacaaat cgcgtttatt ttcaaaataa tgttaaagat
     3181 tatgaacgtg gtaaagtact gagtattatt atgatggata gaggtatgat tccattggga
     3241 agtctattaa tgagtatatg tgcagatgtg tttggcattg ttagaacttt ttcaataatg
     3301 ggaataagta ctatatgcat tacaatggta ttctatttta taaatagaaa gttgaagtta
     3361 aagttggagg aaagtaatca tggtatatct tgaatgggca aaggcagata gaaatattca
     3421 atatcgtgta attaacgcca ttattaaaga acgtatttac cccgagcaaa catttatttc
     3481 gcaaaaagga tctttaattg aaattcagta tcatatgcat gtgttgacta ttgaagttgt
     3541 tagaaaaagt gcattagaac gctatgagtt tacaggtgat attacttatt taaataaagg
     3601 tgaaacgtca ttaattataa ctttagaagg tttattagat gtgttgaatc atgactttga
     3661 tatccctatt tcagagcgac tacgcgaaga gttaatacac agtcgagata gtttagttga
     3721 aacatataag caaatgtctc acagacaaac gttaataagt caaagtttta aattttcaag
     3781 gttaccacaa gatattaact ttttttcatg gttacaacat gtaaaagata gtgataagac
     3841 agatgattta acttattctg agagtttggt accagagggg catccaacac accctttaac
     3901 caaaacgaaa ttgcccttaa ctatggaaga agtacgagca tatgcacctg agtttgaaaa
     3961 agaaatccct ttgcaaatta tgatgattga aaaagaccat gttgtgtgca cagctatgga
     4021 tggtaatgat caatttatta ttgatgaaat aattcccgaa tactacaatc agattcgtgt
     4081 gtttttaaag agtttaggtt tgaaaagtga agactataga gcgattttag tacatccttg
     4141 gcaatatgat catacgatag ggaaatattt tgaagcatgg attgctaaaa aaatattaat
     4201 tccaacgccg tttacaatac tttcaaaagc aactttatca tttaggacga tgtctttaat
     4261 tgataaacca taccatgtta agttgcccgt cgatgcacaa gcaacaagtg ccgttagaac
     4321 agtctcaact gtgactactg tagatggacc aaagttaagt tatgctttac aaaacatgtt
     4381 gaatcaatat ccaggattta aagttgctat ggaaccgttc ggtgaatatg caaatgttga
     4441 taaagatagg gcacgtcagc ttgcttgtat tataagacaa aagcctgaaa ttgatggaaa
     4501 aggtgcaaca gtagttagtg caagtctagt taataaaaat ccaatagatc aaaaagttat
     4561 cgtggatagt tacttagagt ggttaaatca aggaattact aaagaaagta ttacgacatt
     4621 tattgaacga tacgctcaag cattaatccc gcctttaatt gcttttattc aaaattatgg
     4681 aattgcttta gaagcacaca tgcaaaatac agtagtgaac ttggggccac attttgacat
     4741 tcaattttta gtgagagatt taggtggttc tagaattgat ttagaaacat tacaacatcg
     4801 tgtatcagat attaaaatta caaatgatag tttaatagct gattctatag atgcagtgat
     4861 tgcaaaattc caacatgctg ttattcaaaa tcaaatggca gaattaatcc atcattttaa
     4921 tcagtatgat tgtgttgaag aaaccgaatt atttaacata gtacagcaag tagtagcgca
     4981 tgccattaac ccaacactac cacatgcaaa tgagttaaaa gatattttgt ttggaccaac
     5041 aattactgtc aaagcgttgt taaatatgag aatggaaaat aaagtaaagc aatatttaaa
     5101 tattgagtta gataatccga taaaaaaaga ggtgtagtac tacatggcac acgttaacat
     5161 aaatatatcg aagattaaat ataacgccaa agtacttcaa acagtttttc aaagtaaaaa
     5221 tatgcaattc acaccagtaa ttaagtgcat agctggtgac cgtacaattg tagaaagctt
     5281 aaaagcgtta ggtatcaatc atgttgcaga atccagattg gataacataa ttagtattgc
     5341 agatcaggat ttaacatata cgttattgcg tacgcctgcc aaaaaagaga tttcagatat
     5401 gatagaaaaa gttgatatga gtatccaaac agaattatct accatacatc aaataaatga
     5461 agtagcagaa gttttaggaa aaaaacataa gattttatta atggtagatt ggaaagatgg
     5521 ccgtgaaggt gttctgactt atgatgtttt agattatatt aaagagatta ttcacttgaa
     5581 aaatatccat tttgtaggtt tagcattcaa ttttatgtgt tttaaatctg atgcgccaag
     5641 tgatgatgat atctttatga ttaatcgatt cgtctcagct gttgaaagag aaataggata
     5701 tcgattgaaa attatttcag gtggtaattc gagcatgtta ccacaacttt tatataatga
     5761 tttgggtaaa ataaatgaat tgcgaatagg tgaaacgttg tttagaggtg ttgatacaac
     5821 gacaaatcaa gcgatcgcta tgttatatca agatgcgatt acattggagg ctgagatttt
     5881 agaaattaaa cctcgagtta atacacaaac gcatgaatca ttcttacaag ctattgtaga
     5941 tattggttat ttagatacaa aagtcgataa tatttcacct atggatcagc atatcaatat
     6001 tttaggcgct tcgagtgatc atttaatgct tgatttaaat ggtcaaggac actatcaagt
     6061 tggagatcat atatcttttt cattaaatta tgaagcactt tctcattcga tgtatatgaa
     6121 aaatttacat aaggtatata ttgatgattc aaagattgat acgctacttc aaaattttga
     6181 tgtgaaatct cctgctatgg taaaccacta ttaatatatt tatcaataag tctaagttga
     6241 caagttatac tacatatcgt ttgcacggtt gtatcataat tgttcaactt agattttttg
     6301 tatttgttga tttatcaaat taagtgcaac agttcgtcac ataaaattgc aacagataat
     6361 atcagctgaa ttacagggat aacggtcatg ctaaatggtg tcaattgtat taatgcaaag
     6421 atgatatagc aatgatacat tatcagtatt ttgtctaagg aaatgtgcta attgtagtca
     6481 taattattag aggaaatata tagtcataca ttttagaaat acaaaaaaga ttgaacgcta
     6541 cttgacaatg ataattgtta tcaataaaat aataaatgaa gttatacata ttaaggagtg
     6601 gaacgatgag aggtctaaaa acttttagta tattgggatt aatagttgcc ttacttttag
     6661 ttgcagcttg tggtaatacg gataattcaa gtaaaaaaga atcatcaact aaagatacta
     6721 tttcggtaaa agatgaaaat ggtacagtaa aagtacctaa agatgcaaaa cgtatcgttg
     6781 tattagagta ctcatttgca gatgcattag cagcattaga cgttaaacca gttggtattg
     6841 ctgatgatgg taagaaaaaa cgtatcatta aaccagttag agaaaaaatt ggggattata
     6901 cttctgtagg tacacgtaaa cagccaaact tagaagaaat tagtaaatta aaaccggatt
     6961 taattatcgc tgatagcagt agacataaag gtattaataa agaattaaac aaaattgcac
     7021 caacattatc attaaagagt tttgatggag actacaaaca aaatattaat tcgttcaaaa
     7081 caattgctaa agctttaaat aaagaaaaag aaggcgaaaa acgtcttgct gagcatgata
     7141 aattaatcaa taagtataaa gatgaaatta aatttgatag aaatcaaaaa gtgcttccag
     7201 cagtagttgc taaagctggt ttattagcac atccaaacta ttcatatgtt ggacaatttt
     7261 taaacgaact aggatttaaa aatgcattaa gtgacgatgt aacaaaaggt ttaagtaaat
     7321 atttgaaagg accttactta caattagaca ctgaacattt agctgattta aatccagagc
     7381 gtatgatcat tatgacagat catgctaaaa aagattctgc tgaattcaag aagttacaag
     7441 aagatgcaac atggaaaaag ttgaatgcag ttaaaaataa tcgcgtggat attgttgacc
     7501 gtgatgtttg ggcaagatct cgtggcttaa tttcttctga agaaatggct aaagaacttg
     7561 ttgaattatc aaaaaaagaa caaaagtaag gtggaagtaa atggctataa aagaaataag
     7621 tagccaatct gccatagatc ataaaagaaa aagacgcaca acactcacgt atatagtgag
     7681 tttgtgcttt ctttttattt gtatatattt aaatatggcg attggttctt cgaaaattaa
     7741 ttttagcgat atcattcact atgttactgg tcatacagat acgaaagcaa cgtttttatt
     7801 gcataatgta cgtatgccaa ggatgattgc agggttattt attggcggtg cattagcggt
     7861 atctggtttg ttaatgcaag caatgacacg caatccgcta gcatcaccta aaatttttgg
     7921 tgtcagttct ggtgcatcgt ttttcattgt atttgtgacg attatcattc cagcattaga
     7981 atattatgca ttatatttag gtgtgatagg tgcatttata ggtggtttaa ctgtttatac
     8041 actttcaggt gctacgaaag gtatgacacc cattaaatta gcattggctg gtatggccat
     8101 ccatttgttt tttagtagta tgaccgaagg tattattatt ttgaatgaga attcaaacga
     8161 acaagtgatg ttttggcttg tcggttcatt atccagtatg aagtgggatg aaattttaac
     8221 cattctacca tggattattg gtgctttaat tgtaacgatt tttattggac gtcaactaac
     8281 gataatggaa ctaggcgatg acattgctaa gggattgggt caaaatataa ataaagttag
     8341 aattatcatt ggactattag tcattattct tacaggtatg tcggtgtcag tggcaggacc
     8401 aattggattt gttggcttaa tcgtaccgca tatagtcaaa cgctatgtga gtaagaatta
     8461 tttagtcatg attccattaa catttattat aggtgcagat ttgttactac tatcagatgt
     8521 attaagtcgt ttaattacat atccttatga atcaccagta gggattgtta catcattcgt
     8581 aggtgcactt tacttcttat ttataacgat taaaggggtg aaaagaatat gatgacaacg
     8641 aaaattatac gtcgttatag tttaatcaca atccttttaa ttgtgagtat attcataagt
     8701 ttatgtgtag ggtctgtcat gattcatccg attgatgcta ttaagggaat ctttacacaa
     8761 gatgatttta ttttaaatga gtatcgtata cctagaacat tacttggtat tattatcggt
     8821 agtagcttgg ctatttcagg tgcaattata caagctgtaa taagaaaccc gttagcctca
     8881 cctgatgtta taggtatttc taaaggtgcc agtttagctg ctgtgattat tattatgacc
     8941 tttccaacag caccattgtt tgtattacct ataggttcat tcttaggtgc ctttgcagta
     9001 agtttatttt tatcattttt aatttcaaaa tatgatgtta aaggttcaaa gcttgcctta
     9061 attggtttag caataggcgc aatatgtacg gcgattgtgc agttcttatt aatcagaaat
     9121 ccactggaag caaacaaagc gcttgtttgg ttgacaggta gtctatatgg acatgatatg
     9181 tcgaatattt atactatatt accttggttt ttaataacga taccaatcat cattttattg
     9241 agttatcaat tagatgtatt gaatctaggt gatgctgttg caaccgcact cggtttaaaa
     9301 gttaaaacta ttaaaatgct attgttaatt ttagcagtaa tattagcagg atccgcaatt
     9361 tcagttgtcg gtggtattag ctttttagga ttaattgcac ctcatattgc gagacaatta
     9421 ataggaaata aaacattaca cgttattatc atgtcggggt taattggtgc tatattgtta
     9481 acatttggag atggtttagc ccgtggcatt catccgccac tggatattcc tgttggggtt
     9541 atcattgcaa ttatcggtgc accatacttc ttaatattat tacgaagaat gaaataacta
     9601 acatatgatt agagtttaaa aaaggttaag tacgaaatca gtaattgtac ttaacctttt
     9661 ttgaattcat cttgaaaaat tacaagaaat agcgtatatt taataactgt aattattcat
     9721 tattttgcat acataaacat tttgaaagat agaatgataa ggaatatgtt tgttaactga
     9781 atagaattct gtatatactt aaaacgaata catagagaaa tggattgatt ttatggatag
     9841 cttttcaagg aaagagatag ttattgggag acttaagttt attacgatgt ctttaattgg
     9901 tatcttatta tttttagtac caattccagt cgaacaagat ggacagaagc aaacaacgtt
     9961 gcccgtggca tttttagcag gcgtgcttaa agatgtatta ggtggcgtaa tgccattttt
    10021 aattgtgacg ataattactt tatcgggtat cataactctg atttgttcaa ctattttaaa
    10081 agataaattg aaacctgacg ggctaatgaa caatgctttt aacgttagaa ttggttggtt
    10141 aattttacga atattagcag ttgtttttgc atggatgaca tttttacgaa ttggttcaaa
    10201 ggttatttac tcagatgaaa cgggtggatt attgttctca agtttattgc caacattagt
    10261 ggcagtattc ttatttgcag ctctattttt accgctatta atggaatacg gtttgttaga
    10321 gatgttgggt cctattttta gaccggttat gagaccttta tttacattgc caggacgttc
    10381 gacggtagat aatttagcat catttattgg agacggaact gttggtgtct taattacaag
    10441 tagacaatat ggtgaaggat attattctcg tcgtgaggca acagttattt caacgacatt
    10501 tagtgtagtt tctatcacct ttgcaattgt agttgctgaa acagtgcaca tgcaaaatca
    10561 attttttgct ttctatttat cagtgatagt atcctgcctc gttgcagcag tcattatgcc
    10621 aagaatttgg cctttaaata aaattcctga tgagtatgct aaggaggtac ctgaaagtgc
    10681 gcgtactgag gcattgccag aaggaaagac ggcattgaga catggctttg atactgcaac
    10741 agaagtaggt attaaagcac cgggcgttat tgactttttt aagtcaggat taaaaacagt
    10801 cattgatatg tggtttgtta ttttgcctgt agtaatgagt attggtacta ttgctaccat
    10861 tattgcgaac tacacgccct tttttgtcat attgggtaaa ccttttgttc catttttaga
    10921 gttaatgcaa atacctgaag cggcacaggc ttctcaaacg attattatcg gttttgccga
    10981 tatgttttta ccttctatat taattgaagg tgtacaaaat gatataactc gttttgttat
    11041 tggggcactt agtatatcgc agttaatata cttgtctgaa gtaggtggcg tcatacttgg
    11101 ttcgaagatt cctgtaagca tcggcaaatt gtttatgatt ttcttgatta gaacaatcat
    11161 tacactacca attatttctt tgatggcaca tcttttgtta taaatatatg tgaaaaagga
    11221 caactgttaa aattatggga acagttgtcc ttttgtattt gtttatgctg ggtgttgaat
    11281 caattaaaaa aatcattcat gaagccctca ccatgaatga tttttttata caatttattt
    11341 agttaattca ttgtaaactt tttgatcatt ttgagtataa ataatgtatt cgtggtcttc
    11401 agtatcgata ataacgcgat tcgttttacc aaacgttgag ccgattctag atacatcttt
    11461 gctgtctaat ttaggaactg catgaatatc ttggtcttgt gtaatatttt taatttcact
    11521 tgtaggaatt ttgatatcag caactctcca ttgaatacgc acttcattat tatccttttt
    11581 tactgtcatt gccattattc aaacacatcc tttaaatatt ttgtaaatac atcataaacg
    11641 attttgtaat cgttttcaag agttatgtga tactttttta aattgtgtca tactaatgaa
    11701 ttttaacccg aaaatatgtt cgtgtttttg ttagggatgt ggtaaaatgt gagtattaaa
    11761 ggagaacaaa tatgacaggt aaaacacatg cttcatgcgg catgctcgtt ggagcgctaa
    11821 caacacaata ttttcaaacg gatatttttt cgtcggttac ggttatcatt ctagcaactc
    11881 tagctagctt attacccgat atatgtcaca ctcaaagtaa gattggcaga aggtttaagg
    11941 taattagttt ttttgtgaga ttgatctttg gacatcgaac ctttacgcat tcaatattat
    12001 ttatcgcaat catagcattt ttattgcaaa ttattcagac gcctaattat tatatggcag
    12061 caattataat tggattagtg tcacatgtta tactagatat gataacacca aaaggcgtga
    12121 aattatttta tccgttaccg tttaatgtga agacaccgat tcaatttaaa acaggtggac
    12181 ttgtagactt atctttagct acagcgttaa tggtcggaac agtttatgtt ttatttcagc
    12241 ctttcatcaa tgatataata agcaactgga atactaagtt tttatagaaa ataaattatt
    12301 aaaggttaca aactcagcag gaggcataaa atgctagata aaaatcaatt agctaaatat
    12361 aaacaagatc atttgtgtga atatgaaaaa ataatgagta acaatgaaaa agaagcactt
    12421 gaagagaagg tagcgtcatt agatttagat tttattgcca aattatataa cgatttatac
    12481 atcaataaga aaacaataga tgatgtatct gcagtttctg aagtgaaata tgatattaaa
    12541 tcacaaatga gtgatgatga gattaagcgt ttagaggaac aaggacttca agctattaaa
    12601 gaaggacaat ttgcagtact tttgatggca ggtggtcaag gaacaagact tggttacaag
    12661 ggccctaaag gttcgtttga aattgaaggt gtaagtttat ttgaacttca agctaaccaa
    12721 ttaaaaacat tgaatcatca atctggacat acgattcaat ggtatattat gacaagtgat
    12781 atcaatcatg aagaaacttt agcttatttc gaagcacata gttattttgg atatgatcaa
    12841 gaagcaatcc atttctttaa acaagataat attgtggcgc ttagtgaaga agggaagtta
    12901 attttaaatc aacagggtcg tattatggaa acgccgaatg gtaatggtgg cgtgtttaaa
    12961 tctttagaca aggcaggata cttagaagaa atgtctaata atggcgttaa gtatattttc
    13021 ttgaataata tcgacaatgt tttagtaaga gtgttggatc cactatttgc aggatttacg
    13081 gtagaacatg attacgatat tacttctaaa acgattcaac caaaaccagg agaaagtgtt
    13141 ggacgtttag ttaatgtcga ttgtaaagat acagtactgg agtattcaga attagatcca
    13201 gaagttgcaa atcaatttaa caatgctaat atcggtattc atgcatttaa acttggtttt
    13261 attttaaacg ctgtaaatcg tgagttacct tatcatttag ctattaagaa tttgaagcaa
    13321 ttagatgaga actttggtgt tattgaacaa ccaactttaa aattcgaatt gttctatttt
    13381 gacatattta cttatggaac tagttttgtc acgttacaag ttcctagaga agaagaattt
    13441 tcacctctta aaaataaaga aggtaaagat agtgttgcaa ctgcaacaga agatttacgt
    13501 agaatgggtt taatttaatg ataggcggtg actatgatgt ccaaatcaac taaaaaaagt
    13561 aatgacacaa tcgtagaaac atttaaggat atcatccctt tatcatttgg agaagaaatt
    13621 ggtaatgcgg catctcatgg tgttgcagct ttgttaacat tattagtgtt accctatgcg
    13681 gctgtgcaca gttatattgc acatggtacg cttgcgtctg tgagtatgtc tatttatgtt
    13741 atttctatct ttatgatgtt tatttcatct actatttatc attctatgca aaatgaaaca
    13801 cctcataaat atattttaag gattatcgat cacagtatga tttacgtagc aatttctggt
    13861 acatatacgc ctattttgtt aacagttgta ggtggttgga taggttggac agtatttatt
    13921 ttattatggg gaacgacact ttgggggatt ttatataaat ccatagctgt caaagttaac
    13981 caaaagttaa gcttaattgt ctatttagtg atgggctggg taggtattat cttgttacca
    14041 attattttta ttcgcacatc atggatattc atattattca ttttcttagg tggtgtgtct
    14101 tatacaattg gtgcatggtt ttatgcacaa aagaatagac catattttca tatgatttgg
    14161 catattttta ttgtaatagc atcactattg catttaattg caatcttata ttttatgtag
    14221 tcgtttagaa aagctggggg aaactccgct ttttttatta cataaatcat tctaataggg
    14281 gattgtattg taatagaatt atcgttaaga tttgaaagtc aaaagtgttt atacatatga
    14341 aaaccaatta tttgtaaata aaatcttata aataaatata tagaaggagg tatttcatgc
    14401 gattaaagtc aatcataact gttatagctt taatattaat tatgtttatg tcagcgattg
    14461 aatcatctat tatttcgctt gcattaccta caattaaaca agatttaaat gcaggaaatt
    14521 taatttcatt aattttcaca gcgtatttta tcgcattagt aattgccaat cctatagtgg
    14581 gcgaattact ttcgagattt aaaattattt atgtggcaat tgcaggatta cttttattta
    14641 gtataggtag ttttatgtgt ggtttaagta ctaattttac tatgctaatt atatcacgcg
    14701 tgatacaagg ttttggatca ggtgtattga tgtcattatc gcaaattgtt ccaaagttag
    14761 catttgaaat tccgttacga tataaaatta tgggcatagt tggcagtgtt tggggtattt
    14821 cgagtatcat tgggccactt ctaggtggtg gtatattaga atttgcaaca tggcattggt
    14881 tattctacat aaatataccg attgcgatca ttgccattat attagttata tggacatttc
    14941 attttccaga ggaagaaaca gttgctaaat caaaatttga tactaaaggg ctcacgctat
    15001 tttatgtatt tattggattg attatgtttg ctttattaaa ccaacagctg ttacttttaa
    15061 atttcttgag ttttatctta gctatcgttg tggcaatgtg tttatttaaa gtagaaaaac
    15121 atgtttcttc accattttta cctgtggttg aatttaatcg ttcgattact ttagttttta
    15181 taactgacct tctaacagct atttgtttaa tgggattcaa tttatatatt ccagtctacc
    15241 ttcaagaaca actaggatta tctccattgc aaagtggatt ggttattttt cctttatctg
    15301 tagcttggat tacattgaat tttaatttac atcgaattga agcaaaacta tcaaggaaag
    15361 ttatttactt actatcattt acattgctac tagtaagtag tattatcatt tcatttggta
    15421 ttaaattgcc ggtacttata gcatttgtgt taattttggc aggattaagt tttggatata
    15481 tttatacgaa agatagtgtg attgtccaag aggaaactag cccattacaa atgaagaaaa
    15541 tgatgtcatt ttatggatta actaaaaatc ttggggcatc aataggttca acaataatgg
    15601 gatatcttta tgcgatacaa tcaggaatct ttggtccaaa cttacacaat gtgttaagtg
    15661 ctgttgctgt aattagcatt gggcttattg ttttatgggt cgtatttttt aaagaacagt
    15721 catctcaatc aaaagaatag aattatgatt aaaattgatg tagtgtaatt aaagtaagaa
    15781 ataataaatt tcctagatac tagataaatc gttcaatatt ttattaataa ggaggccaat
    15841 gattgtgaat tatcttaaac ataagtttta taacttgtta actacaatga ttgttctctt
    15901 tatttttgta ctttctggtg cgattttttt aacatttcta gggtttggtt tatatggatt
    15961 aagtagaata cttatttatt ttaggttggg tgactttaca tataatagaa gtatgtacga
    16021 taacctatta tattatggca gttatataat ctttgggtac ttcatcatat ttgcagtcga
    16081 gcatttaatg gattatttta gaaaaatgtt gcctgaaaat gcatatttta gaggcgcgac
    16141 ttttcatttg atttcttaca cagttgcaac aacgttattt tattttatta ttcatctgaa
    16201 ctatgtgtat atcaatatcg atttttgggt catcatggtt attatagggt tcttatatgt
    16261 ttgtaagctt caattttacc ctgaaagtaa aaatttaaat aatagaaaat agatatctga
    16321 agttgagggc acatatgtgt tccaacttct ttttgttcaa tagaagaatc gataatagtg
    16381 agtcttagtg ctcaatttgt ggtaaataag ttaaataacc attgtatgag tctgagacat
    16441 aaaataaatt attaggccct acaatgttat attgttagtt tttaagtaca cattagctgt
    16501 gcctaatgtg taagaaccaa taatgattta ttatgtagtg cttctttatt atttgagtac
    16561 tactcccatt atgttcatgg ctatacgtat taaagggaca agtgtatatc tgttttaaaa
    16621 aggaggaatt atgatggcta aagttgaatt aacaacccgg agacgaaact tcatagttgc
    16681 tgttatgttg attagtgcgt ttgtagctat tttaaatcaa acattattaa atacagcgtt
    16741 acctagtata atgagagaat taaatatcaa tgaaagtaca tcgcaatggc tagttactgg
    16801 gtttatgctt gttaatggcg tcatgatacc tctgacggca tatctaatgg atagaattaa
    16861 aactagacct ttatacttag cggcgatggg gacattttta ttaggttcta ttgttgcagc
    16921 cttagctccg aattttggag ttttaatgtt agctcgtgta attcaagcga tgggtgcagg
    16981 cgtacttatg cccttaatgc aatttacgtt atttacattg ttcagtaaag aacatcgagg
    17041 ttttgcaatg ggactagcag gtttagtaat tcaatttgca ccagcaatag gacctacagc
    17101 tacaggatta attattgatc aagcgagttg gcgagttcca tttattataa ttttaggaat
    17161 tgctatactt gcctttgttt tcggtttggt ttcaatctcg agttacaatg aagtgaaata
    17221 tacgaaatta gataagcgtt cagtaatgta ttcaactatt gggttcgggt taatgctata
    17281 cgcatttagt agtgcaggag atttaggatt tacaagtcca atagtaatag gtgcgttgat
    17341 attaagtatg gttattatct atttatttat acgtagacaa tttaatatta ctaatgcact
    17401 tttaaattta agggttttta aaaatagaac atttgcatta tgtacgatta gttcaatgat
    17461 tataatgatg tcaatggttg gacctgcgct gcttataccg ctatatgttc aaaacagttt
    17521 atctttatct gccttgttat caggacttgt tatcatgcct ggtgcaataa taaatggtat
    17581 tatgtcagtt tttacaggta aattttatga taagtatggt cctagaccat tgatttatac
    17641 tggttttaca attttaacaa ttactacaat tatgttgtgt ttcttgcata cagacacatc
    17701 ttatacgtat ttaatagtcg tatatgcaat tagaatgttt tcagtttctt tactcatgat
    17761 gccgataaat actacaggaa ttaattcttt gagaaatgaa gaaatctcac atggcacggc
    17821 tattatgaac tttggtcgtg taatggctgg ttcactaggc acagctttaa tggttacatt
    17881 aatgagtttt ggtgcaaaaa tatttttatc tacatcgcca tcgcatttaa ctgcaactga
    17941 aattaaacag caatccattg ctataggggt ggatatctca tttgcttttg tagctgtgct
    18001 tgttatggca gcttatgtga tagcactttt tataagagaa cctaaagaaa tagaaagtaa
    18061 tagaaggaaa ttttaaaata attatagtag ttggtctatt taaaataata ggctaactgc
    18121 tttttttatt taataaaaag ttttatactt ttagtgatag actaagcaaa aattgttatt
    18181 tgctatgatg tagatgtctt aaaatgatta agggggattt gctttgttaa cggtagatca
    18241 agtgaaagaa ttggtaggag aaattaaaga tcctattata gatgtgcctt taaaagaaac
    18301 agaaggtatt gttgaagttt ctattaagga agaaaaagaa catgtgagtg ttaaattagc
    18361 aatggcacaa ttaggtggtg caccgcaatt agatttacag atggctgttg ttaatgcatt
    18421 aaaagaaaac ggtgcgaaaa cggtcggtat acgatttgaa acattgccgg aagaaaaagt
    18481 aaatcaattt aaaccaaaag aagaaaataa acctaaaacg atagaaggcc tattatctca
    18541 aaataatcca gtcgaattta ttgcaatagc ctccggtaaa ggtggtgtcg gtaaatctac
    18601 tgttgcagta aatttagccg ttgccttagc tcgtgaaggg aaaaaagtcg gattagtaga
    18661 tgccgatata tatggattta gtgtaccaga tatgatgggt attgatgaaa agcctggaat
    18721 taaagggaag gaagtaattc cagttgaacg tcatggcgtt aaagttatat caatggcctt
    18781 ttttgtggaa gaaaatgcgc cagttatatg gagagggcca atgttaggta aaatgttgac
    18841 gaatttcttt acagaagtta aatggggaga cattgaatat ttaatactcg atcttccacc
    18901 tggaacagga gatgtagctt tagatgttca tacgatgtta ccttcaagta aggaaattat
    18961 tgtaacgaca cctcatccta cagcagcatt tgttgcagct cgcgcaggtg cgatggcaaa
    19021 acatacggat cattctattc ttggcgtaat tgaaaacatg agttattttg aaagtaaaga
    19081 gacgggtaat aaagaatatg tctttggcaa aggtggcggt actaagttag ctgatgaact
    19141 taatactcaa ttacttgggg aattaccttt agagcaacca tcttggaatc caaaagattt
    19201 tgcaccgtca atatatcaat cagatgatcg tctaggtaaa atttatagct cgattgcaca
    19261 aaaagttata gctgctacaa ataaataggt ataatatatg ctaaacgtac aaaattactt
    19321 ttattttgta cgtttttatg ttgatttgtt ttggaatgct ggtaaaataa gttcttgtgt
    19381 taagcaataa acgcgttgaa aaagaagcgg gttgtgaaca caaaaagttt ttaaaaaagt
    19441 tattgacttt aaattccaaa gttgataata tgataaagtc gtcaaaaacg acgcagttgt
    19501 ttttaaagaa gaatttcaaa agtgtaaaac ttaccattga aataactata aaaacaatat
    19561 agaataaata aagtaatcgt tcaaaaagtt attgacttca agaaataaat gaagtagaat
    19621 aaaagagatt gaacattaat gaacattgaa aactgaatga caatatgtca acgttaattc
    19681 caaaaacgta actat
//
LOCUS       DALAGD010000058        19642 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.058, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000058 DALAGD010000000
VERSION     DALAGD010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19642
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.058"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            76..540
                     /locus_tag="O2701_001666"
     CDS             76..540
                     /locus_tag="O2701_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ4293557.1"
                     /translation="MDRQSFTDLIQTKFKMVRIEAGYTQDTMAQTIGLSKKTLVQIEK
                     ERVLPNWTTCISICALFRDSEVLNSTFGCDPLEIVQTISRNHCAYPNHAPTSDIYWNN
                     IETRNGYILQSNKVSNIYRVLNPDNQPIFGTSKMREAETYFNRNAKEELVHI"
     gene            719..1843
                     /locus_tag="O2701_001667"
     CDS             719..1843
                     /locus_tag="O2701_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HCZ4293558.1"
                     /translation="MNALFIIIFMIVVGAIIGGITNVIAIRMLFHPFKPYYIFKFRVP
                     FTPGLIPKRREEIATKIGQVIEEHLLTETLINEKLKSEQSQQAIESMIQQQLQKLTKD
                     QLSIKQITSQIDIDLEQVLQTNGNQYIESQLNNYYTKHQNQTIASLLPNQLVTFLNQH
                     VDNATDLLCDRARNYLSSAKGTQDINDMLDTFFNEKGKLIGMLQMFMTKESIADRIQQ
                     ELIRLTSHPKARTIVTSLITNEYQTFKDKPLNELLDASQFNEIAENLSVYVTTYASKQ
                     ANKPVVTLMPQFVDYLEGQLSSKLANLIIEKLSIHLSTIMKKVDLRGLIEEQINTFDL
                     DYIEKLIIEIANKELKLIMSLGFILGGIIGFFQGLVAIFV"
     gene            1912..2256
                     /locus_tag="O2701_001668"
     CDS             1912..2256
                     /locus_tag="O2701_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HCZ4293559.1"
                     /translation="MAVNLYDYANQLEQALRESEEYKAIKEAFANVKANEESKKLFDE
                     FRETQINFQQKQMQGEEIAEEDLQKAQEQAQAIEKDENISALMNAEQKMSQVFQEINQ
                     IIVKPLDEIYAD"
     gene            2639..2737
                     /locus_tag="O2701_001669"
     CDS             2639..2737
                     /locus_tag="O2701_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293560.1"
                     /translation="MPFDSLAVLITTEIAFPTEIFEKYQRTLQIKF"
     gene            3028..4224
                     /locus_tag="O2701_001670"
     CDS             3028..4224
                     /locus_tag="O2701_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCZ4293561.1"
                     /translation="MVKFIHCSDLHLDSPFKSKSHISPKIFEDVQKSAYESFKNIVDI
                     ALQQDVDFVIIAGDLFDSENRTLRAEIFLKQQFERLQNEQIFVYVCHGNHDPLSSKIS
                     SNWPDNVSVFSNKVETYEAITKSGETIYIHGFSYENRASYENKIDEYPSSQGQKGIHI
                     GVLHGTYSKSSVNERYTEFILEDLNSKLYHYWALGHIHERQQLSDMPVINYSGNIQGR
                     HFNEQGEKGCLLIEGDHLKLKTKFYPTQYIRFEEATIETDKTSKQGLYEVIQNFKEQV
                     REEGKAFYRLTLVINSETLISPQDLLQVEEMITDYEENENQFVYIDELKIQYAQNDES
                     PLVNEFSAELLVDQTVFDKAMSDLYLNPRASKFLDDYGTFDHTALVNRAEEILKAEMR
                     GEQNDN"
     gene            4214..7150
                     /locus_tag="O2701_001671"
     CDS             4214..7150
                     /locus_tag="O2701_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCZ4293562.1"
                     /translation="MIIKSLEIYGYGQFVQRKIEFNKNFTEIFGENEAGKSTIQAFIH
                     SILFGFPTKKSKEPRLEPRLGNQYGGKLVLILDDGLEIEVERIKGSAQGDVKVYLPNG
                     AVRDDAWLQKKLNYISKKTYQGIFSFDVLGLQDIHRNLNEKQLQDYLLQAGALGSTEF
                     TSMREVINRKKDELYKKSGKNPIINQQIEQLKQLESQIREEEAKLETYHRLVDDRDKS
                     SRRLENLKHNLNQLSKMHEEKQKEVALHDHSQEWKSLEQQLNIEPITFPEKGVDRYEK
                     ARAHKQSLERDIGLRNERLAQLKEEATQLEPVKQSDIDAFISLNQQENEIKNKEFELT
                     AIEKDIANKQRDKDELQSNIGWSETHHDVDSSEAMKSYVSEQIKNKQEQAAYIKQLER
                     SLEENKIEDNAVHSELDSVEEKIVPEETFEKKKEYSQQVIELNEKENLYSKLKERFEI
                     EQQEKQKRQKLLRTTFILLTLVGIGLTAFSFISNNMLFGIIFAVLTLVFVIGIIMSKS
                     KEVDYSEAITDEIEEIKAQLAILDENYDLDFDLDEQYRIRDHWQQALKNKDILEEKRQ
                     YIEGRLNDAKGRHDELQSTVENVKDELYLSSKISNDLIVDSISTMANIKALDQHISDL
                     NQQRQQLVQELDTFYNHAEAVTKSQFVYFNKLSLFHDVQQWLKSAEDTNEKWRINAEN
                     TKLVTNELNHLNAQLEENNKEITALFDFINVGTEEDFYQHHEDYQTYTSNLSRFNDLT
                     KYLENQNYSYELSSSLSEKTTAQLEEEDHLLATQVDEYNEQYLEMQAQVSDLSAQINH
                     METDTTLANLRHEYHSLKNQLNDIAKDWASLSYLQSLVDEHIKQIKDKRLPQVINEAV
                     EILKHLTDGRYTMINYNEDSITVKHVNGQLYDPVELSQSTKELLYVALRISLIKVLRP
                     YYPFPLIVDDAFVHFDKKRTEKMLNYLRSLSEHYQVLYFTCVKDNIVPSKEVITLNKI
                     EEGGKR"
     gene            7147..8088
                     /gene="yhaM"
                     /locus_tag="O2701_001672"
     CDS             7147..8088
                     /gene="yhaM"
                     /locus_tag="O2701_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM"
                     /protein_id="HCZ4293563.1"
                     /translation="MRNIENLNPGDSVDHFFLVHKATQGVTAQGKDYMTLHLQDKSGE
                     IEAKFWTATKNDMATIKPEEIVHVKGDIINYRGNKQMKVNQIRLATTEDQLKTEQFVD
                     GAPLSPAEIQEEISHYLLDIENANLQRITRHLLKKYQERFYTYPAASSHHHNFASGLS
                     YHVLTMLRIAKSICDIYPLLNKSLLYSGIILHDIGKVRELSGPVATSYTVEGNLLGHI
                     SIASDEVVEAARELNIEGEEIMLLRHMILSHHGKLEYGSPKLPYLKEAEILCYIDNID
                     ARMNMFEKAYKKTDKGQFTDKIFGLENRRFYNPESLD"
     gene            complement(8209..9171)
                     /locus_tag="O2701_001673"
     CDS             complement(8209..9171)
                     /locus_tag="O2701_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HCZ4293564.1"
                     /translation="MKMINKLIVPVTASALLLGACGASATDSKENTLISSKAGDVTVA
                     DTMKKIGKDQIANASFTEMLNKILADKYKNKVNDKKIDEQIEKMQKQYGGKDKFEKAL
                     QQQGLTADKYKENLRTAAYHKELLSDKIKISDSEIKEDSKKASHILIKVKSKKSDKEG
                     LDDKEAKQKAEEIQKEVSKDPSKFGEIAKKESMDTGSAKKDGELGYVLKGQTDKDFEK
                     ALFKLKDGEVSEVVKSSFGYHIIKADKPTDFNSEKQSLKEKLVDQKVQKNPKLLTDAY
                     KDLLKEYDVDFKDRDIKSVVEDKILNPEKLKQGGAQGGQSGMSQ"
     gene            complement(9376..9933)
                     /locus_tag="O2701_001674"
     CDS             complement(9376..9933)
                     /locus_tag="O2701_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HCZ4293565.1"
                     /translation="MFLCKRQIDINARFGLPRIAFMSAVATIIMFLVSYEVMYFLSNT
                     PLSDRHFLIFLLLVFMTYPLHKSIHLLFFLPYRKSFKVHKLTKRKWLIFYNTYVNQPV
                     HKFYFCINLILPLIILSAMFVYLTISFPQYGHYFMFLLALNFGISITDLLYLKIIIFS
                     NYGQYIEEHSTGINILKKIKNPYHL"
     gene            complement(10666..11031)
                     /locus_tag="O2701_001675"
     CDS             complement(10666..11031)
                     /locus_tag="O2701_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCZ4293566.1"
                     /translation="MKASRILFGIGVGVAAGFVVALQGRDDKSVKNNTIDRTAPTGSK
                     SELQREFETIKQSFNDILNYGVQIKNESAEFGSSIGGEIKSLLGNFKSDINPNIERLQ
                     SHIENLQNRGEDIGNEISK"
     gene            complement(11173..11595)
                     /locus_tag="O2701_001676"
     CDS             complement(11173..11595)
                     /locus_tag="O2701_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HCZ4293567.1"
                     /translation="MSETIFGKILTGEIPSFKVYEDDYVYAFLDISQVTKGHTLLIPK
                     KASANIFETDEETMKHIGAALPKVANAIKRAFNPDGLNIIQNNGEFADQSVFHIHFHL
                     IPRYENDIDGFGYKWETHEDILDNDAKQQIAEQIQAQF"
     gene            11729..12469
                     /locus_tag="O2701_001677"
     CDS             11729..12469
                     /locus_tag="O2701_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4293568.1"
                     /translation="MTVKVEQLTGGYGKRPVIKDINFELNKGEIVGLIGLNGAGKSTT
                     IKHMLGLLTPMEGSLSISDININDDIEAYRRKLSYIPESPVIYEELTLEEHIEMTAMA
                     YDIDRDETMNRAMPLLKTFRLENELKVFPSHFSKGMKQKVMIICAFIVNPELYIIDEP
                     FLGLDPLGIQSMLDLMVEKKNEGRTVLMSTHILATAERYCDRFIILDEGEVVAFGDLE
                     ALRQQTGLHNQTLDDIYIHVTQGGDVHA"
     gene            12462..13685
                     /locus_tag="O2701_001678"
     CDS             12462..13685
                     /locus_tag="O2701_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4293569.1"
                     /translation="MRNQATTLFNKRLHALRKEKNYYNKFIFNGHFMVFLLILLGAFI
                     FGYGEWLKHIPTNINFSLIAAVIVALTSIFPMRPLLKEADKIFLLPFEKHMSQFMRHA
                     ILYSYFARILIQLIIVIVMFPLFYNINQHNVAFYICFGVSALIFPYVGLRLRWQWYQS
                     GLKTWQVNLISFITFALTYYLLLAPKWYIAFVMVALPVLIEFLVKKYKPGFLYPWEKM
                     IAIEHRHHMNYYKFVNMFTDVKHLKESAVRRSYLDILLPVPKGSKFNSNAMYLFLFIR
                     SFIRGRDAFNIIFRLVIIAVLLMVWLSYPLVTAIIGCLFVYIILLQMAQFYSQQAYGL
                     WPQVWPVPEEKVIKGYEQFLYRLMFVICTVFAVTFIIKHMTLFYVVLIFYIVGLLTIR
                     SIIKKLKYQETLLRD"
     gene            complement(13809..14312)
                     /gene="traP"
                     /locus_tag="O2701_001679"
     CDS             complement(13809..14312)
                     /gene="traP"
                     /locus_tag="O2701_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein TRAP"
                     /protein_id="HCZ4293570.1"
                     /translation="MKKLYTSYGTYGFLHQIKINNPTHQLFQFSASDTSVIFEETDGE
                     TVLKSPSIYEVIKEIGEFSEHHFYCAIFIPSTEDHAYQLEKKLISVDDNFRNFGGFKS
                     YRLLRPAKGTTYKIYFGFADRHAYEDFKQSDAFNDHFSKDALSHYFGSSGQHSSYFER
                     YLYPIKE"
     gene            14475..14585
                     /locus_tag="O2701_001680"
     CDS             14475..14585
                     /locus_tag="O2701_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293571.1"
                     /translation="MIKCQFYDIYLLKIRTNERYDNIDKNDFNLGGLYGA"
     gene            14575..15612
                     /gene="hemE"
                     /locus_tag="O2701_001681"
     CDS             14575..15612
                     /gene="hemE"
                     /locus_tag="O2701_001681"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCZ4293572.1"
                     /translation="MVHNKNNTILKMIKGEETSHTPVWFMRQAGRSQPEYRKLKEKYS
                     LFDITHQPELCAYVTHLPVDNYHTDAAILYKDIMTPLKPIGVDVEIKSGIGPVIHNPI
                     KTIQDVEKLSQIDPERDVPYVLDTIKLLTEEKLNVPLIGFTGAPFTLASYMIEGGPSK
                     NYNFTKAMMYRDEATWFALMNHLVDVSVKYVTAQVEAGAELIQIFDSWVGALNVEDYR
                     RYIKPHMIRLISEVKEKHDVPVILFGVGASHLINEWNDLPIDVLGLDWRTSINQAQQL
                     GVTKTLQGNLDPSILLAPWNVIEERLKPILDQGMENGKHIFNLGHGVFPEVQPETLRK
                     VSEFVHTYTQR"
     gene            15670..16593
                     /gene="hemH"
                     /locus_tag="O2701_001682"
     CDS             15670..16593
                     /gene="hemH"
                     /locus_tag="O2701_001682"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCZ4293573.1"
                     /translation="MTKKMGLLVMAYGTPYKESDIEPYYTDIRHGKRPSEEELQDLKD
                     RYEFIGGLSPLAGTTDDQADALVSALNKAYADVEFKLYLGLKHISPFIEDAVEQMHND
                     GITEAITVVLAPHYSSFSVGSYDKRADEEAAKYGIQLTHVKHYYEQPKFIEYWTNKVN
                     ETLAQIPEEEHKDTVLVVSAHSLPKGLIEKNNDPYPQELEHTALLIKEQSNIEHIAIG
                     WQSEGNTGTPWLGPDVQDLTRDLYEKHQYKNFIYTPVGFVCEHLEVLYDNDYECKVVC
                     DDIGANYYRPKMPNTHPLFIGAIVDEIKSIF"
     gene            16617..18017
                     /locus_tag="O2701_001683"
     CDS             16617..18017
                     /locus_tag="O2701_001683"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HCZ4293574.1"
                     /translation="MTKSVAIIGAGITGLSSAYFLKQQDPNIDVTIFEASNRPGGKIQ
                     SYRKDGYMIELGPESYLGRKTIMTELAKDIGLEQDIVTNTTGQSYIFAKNKLYPIPGG
                     SIMGIPTDIKPFVTTKLISPLGKLRAGFDLLKKPTQMQDGDISVGAFFRARLGNEVLE
                     NLIEPLMGGIYGTDIDKLSLMSTFPNFKEKEEAFGSLIKGMKDEKNKRLKQRQLYPGA
                     PKGQFKQFKHGLSSFIEALEQDVKNKGVTIRYNTSVDDIITSQKQYKIVYNDQLEEVY
                     DGVLVTTPHQVFLNWFGQDPAFDYFKTMDSTTVATVVLAFDEKDIENTHDGTGFVIAR
                     TSDTDITACTWTSKKWPFTTPEGKVLIRAYVGKPGDTVVDDHTDNELVSIVRRDLSQM
                     MTFKGDPEFTIVNRLPKSMPQYHVGHIQQIRQIQAHIKQTYPRLRVTGASFEAVGLPD
                     CITQGKVAAEEVIAEL"
     gene            18340..18894
                     /locus_tag="O2701_001684"
     CDS             18340..18894
                     /locus_tag="O2701_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase family protein"
                     /protein_id="HCZ4293575.1"
                     /translation="MTDVIIVHSMHGNSRNHWYQWLEHNLTLEGYDVTLFNFESPEAK
                     TVDQWIEAMTKQINVRKKDTYFVTHGLGSITALKYIEMIDQPIEGFFSIAGFKEDAEN
                     IDLDIDLSNVTIDYDNIKKKVDNFYGLSSKNDKYVSYKETQRLMNALEGNLRIVEDGG
                     HFLEEDGFETFTALQDRMQDYMTR"
     gene            19366..19439
                     /locus_tag="O2701_001685"
     tRNA            19366..19439
                     /locus_tag="O2701_001685"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19400..19402,aa:Met,seq:cat)
     gene            19461..19536
                     /locus_tag="O2701_001686"
     tRNA            19461..19536
                     /locus_tag="O2701_001686"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19494..19496,aa:Asp,seq:gtc)
     gene            19553..19625
                     /locus_tag="O2701_001687"
     tRNA            19553..19625
                     /locus_tag="O2701_001687"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19586..19588,aa:Phe,seq:gaa)
BASE COUNT         7078 a         2771 c         3460 g         6333 t
ORIGIN      
        1 tgtttcaaga aatgtgtatt attgcaattt cttgtgtccg gataaaattc cccaaacaga
       61 aaggtagata acagaatgga tagacagagt tttacagatt taattcaaac aaaatttaaa
      121 atggttcgta tagaggctgg ttatacgcaa gatactatgg cgcaaacaat tggactttct
      181 aaaaagactt tagtacaaat cgaaaaagag agagtattac caaactggac aacttgtatt
      241 tcaatttgtg cattattcag agactctgaa gtattaaaca gtacatttgg ctgtgatcca
      301 ttagaaatcg ttcaaacaat ttcaagaaat cactgtgcat atccaaacca tgcaccaaca
      361 agtgatattt attggaataa cattgagact cgtaatgggt acattttaca aagtaacaaa
      421 gtaagcaata tttatcgtgt attaaatcca gataaccaac caattttcgg tacttctaaa
      481 atgagagaag ctgaaacata ctttaacaga aatgctaaag aagaattagt acatatctag
      541 gcctatatac ttttaataaa taataaaagc gatgtaacac acctgtttcg agaggtgagg
      601 catcgctttt tgtatgtaaa ctaataataa attgagcaga aagtgaaatg attaatgaat
      661 taaaaaataa aaatagtggt ataatgaata actatgttgt cacaaaggag tcaaaattat
      721 gaatgcacta tttattatca tatttatgat tgtagtaggg gcgataattg gtggcattac
      781 taatgtaatt gcaattagaa tgctgtttca cccctttaaa ccatactata tatttaaatt
      841 tagagtccca tttacaccag gtttaatacc gaaaagacgc gaagaaattg caactaaaat
      901 tggccaagtg attgaagagc atttgcttac agaaacttta attaatgaaa aattgaaaag
      961 cgagcaatca cagcaagcaa tagaatctat gattcaacag cagttacaaa agttgacgaa
     1021 agatcaattg tcaataaaac aaattacttc tcaaatcgat attgatttag aacaagtatt
     1081 acaaactaat ggaaatcaat atattgaatc acaattgaat aattattata caaagcatca
     1141 aaaccaaaca atagcatctt tattgccaaa tcaacttgta acatttttaa atcagcatgt
     1201 agataacgca acagacttat tatgtgatcg tgcaaggaat tatttatcat ctgcaaaagg
     1261 cacacaagat attaatgata tgttggatac ttttttcaat gagaaaggta agttaattgg
     1321 tatgttgcaa atgtttatga caaaagagag tattgcagat cgcattcaac aagaacttat
     1381 acgtttaaca tctcatccta aagcaagaac aattgtgaca tcgttaatta ccaatgaata
     1441 tcaaactttt aaagataaac cattgaatga actgttagat gcatcgcagt ttaatgaaat
     1501 cgctgaaaat ctatcagtgt atgtaacaac atatgcaagc aaacaagcga ataagccagt
     1561 ggttacgtta atgcctcaat tcgttgatta tttagaaggt caactctcta gcaaattagc
     1621 aaatttaatc attgaaaaat tatcgattca cttatctaca attatgaaaa aagtggattt
     1681 acgaggatta attgaagaac aaattaatac gtttgactta gattatattg aaaaattaat
     1741 tatagaaatt gctaacaaag aactaaagtt gattatgtca ttaggtttca ttttaggtgg
     1801 tattattgga tttttccaag gtttggttgc aatctttgtc taaaaaagcg agatgtggta
     1861 aagtaatatc gaacgtttta ataaacatca caacaaagga gtgctataac catggcagta
     1921 aatttatatg attatgcaaa tcaattagaa caagctttaa gagaaagcga agaatacaaa
     1981 gcaatcaaag aagcattcgc taatgtaaaa gctaacgaag aatctaaaaa gttattcgac
     2041 gagttccgtg aaactcaaat taacttccaa caaaaacaaa tgcaaggtga agaaattgct
     2101 gaagaagatt tacaaaaagc gcaagaacaa gcgcaagcaa ttgaaaaaga tgaaaacatc
     2161 tctgcattaa tgaatgctga acaaaaaatg agtcaagtat tccaagaaat caaccaaatt
     2221 atcgttaaac cattagacga aatttacgct gactaatatt aaaataatat ttttcaatcc
     2281 tttaaataga ggaatagact gccgacagag tccgagactt tgtgggtagt ttttttagtt
     2341 ttgataacgg aagttagagg ctctctgtca aattgggcag atgcattcag taacggaagt
     2401 taggctagta aacgtgtcct aacttccttt ttgcattttg aagtgtaaag ttttcaacat
     2461 aatactatta gttcggtcat gtatcggact gatggaaaag cgtttcactt ttaatgactc
     2521 attaagaacg gcctgaaaat gtttggcgta ttaagtgcaa tgatagtttt gacatttagt
     2581 ttctaattgg tcattactgc cgagcaaatc tagtagagta atcatgtaaa tctttaatgt
     2641 gccatttgat tcactagcgg tgttaataac tacggaaatt gcatttccga ctgaaatttt
     2701 tgaaaaatat caacgtacgc tacaaataaa attttaaact gttataaatg tgtctcaatt
     2761 tcatatgttc atcgacgata tgaagcgtat tatggtaaaa tgaagaaata ataaacttgt
     2821 taataaataa aacatcacga tttgactaaa gcactttatt attgtgtaga taatagtttt
     2881 ttaacgaaat aaaaatggcg actggtttta ataaatcagc taatgaatca ctacacctat
     2941 aagtatgaat atagtgatta gaatgctttg tatagttgga ttttgcaaaa ttgatgttag
     3001 aacctttaat taaggagata tggatgtatg gttaaattta ttcattgttc ggatttacat
     3061 ttagatagtc ctttcaaatc taagagtcat ataagcccta aaatttttga ggatgttcaa
     3121 aaaagtgctt atgaaagttt taaaaatatt gtagatattg cattacaaca agatgttgat
     3181 tttgtaatta tcgcgggtga tttatttgat agtgaaaaca gaacattacg tgctgaaatt
     3241 ttcttaaaac agcaatttga acgtttacaa aatgaacaaa tctttgttta tgtttgtcat
     3301 ggtaatcacg atcctttatc atcaaagatt tcatcaaact ggccagataa tgtttctgta
     3361 ttttcaaata aagttgagac gtatgaagca attactaaat ctggtgaaac aatttatatt
     3421 cacggattta gttatgaaaa tagagcaagt tatgagaaca agattgatga atatccatca
     3481 agtcaaggcc aaaaaggcat acatattggt gtcttgcatg gtacgtatag taaatcttca
     3541 gttaacgaaa gatataccga gttcatttta gaagatttaa acagtaaatt gtatcattat
     3601 tgggctttag gtcatataca tgaacgtcaa caattaagtg atatgcctgt aattaactat
     3661 tcaggtaata ttcaaggtag acattttaat gagcaaggtg aaaaaggttg cttattaatc
     3721 gagggtgacc acttaaaatt aaagactaaa ttttatccta cacagtatat tagatttgaa
     3781 gaagcaacta ttgaaacgga taagacatct aagcaaggtt tatacgaggt cattcaaaac
     3841 tttaaagaac aagtgagaga agaaggaaaa gccttttatc gtttaacgct tgttattaat
     3901 agtgagacat taatttcacc tcaagattta ttacaagttg aagaaatgat tacagattat
     3961 gaagaaaacg aaaatcaatt tgtatatatt gatgagttaa aaatacaata tgcacaaaat
     4021 gatgagtcac ctttagttaa tgaattttca gcggaattat tagtcgatca aactgttttt
     4081 gataaagcga tgtcagattt atatttaaat ccaagggcat ctaagttcct agacgattat
     4141 ggaacattcg accatacagc attagttaat cgtgctgaag aaatattaaa agctgaaatg
     4201 agaggtgaac aaaatgataa ttaaatcact tgaaatttat ggttacggtc aatttgttca
     4261 acgtaaaatt gaatttaata aaaacttcac tgaaattttt ggtgaaaatg aagcgggtaa
     4321 atcgacgatt caagcattca tccattcgat attatttgga tttccaacta aaaagtctaa
     4381 agagccaaga ctagaaccac gtctaggtaa ccaatacggt ggtaaattag tacttattct
     4441 tgatgatggc ttagagattg aagttgaacg aattaaaggc agtgctcaag gtgatgtgaa
     4501 agtatattta cctaatggtg ctgtgcgtga tgatgcttgg ttacaaaaga aacttaatta
     4561 tatttctaaa aagacatatc aaggtatctt ttcatttgat gtactagggc ttcaagacat
     4621 tcatagaaat ctaaatgaaa aacaattgca agattattta ttacaagcag gggctttagg
     4681 atcaactgaa ttcacgtcaa tgcgcgaagt gattaatcgt aaaaaagatg aattatataa
     4741 aaaatcaggt aaaaatccga tcattaatca acaaattgag caattaaaac aactagaaag
     4801 tcaaattcgt gaagaagaag caaagctaga aacatatcat cgcttagtag atgatcgaga
     4861 taaatcatca cgtcgattag agaatttaaa gcataattta aatcaattat caaaaatgca
     4921 tgaagaaaaa caaaaagagg ttgctttaca tgatcattca caagaatgga agtctctaga
     4981 acaacagtta aatattgagc caatcacatt cccagaaaaa ggtgtggatc gttacgaaaa
     5041 agcacgagcg cataagcaat cgttagaaag agatattggt ttaagaaatg agcgtttagc
     5101 tcaacttaaa gaagaagcga ctcaattaga gccagttaaa caatctgata ttgacgcctt
     5161 cattagtttg aatcaacaag aaaatgaaat taaaaataaa gaatttgaac ttactgcaat
     5221 cgaaaaggat attgcgaata aacaacgtga taaagatgaa ttgcaatcaa atattggttg
     5281 gtctgaaacg catcatgacg tagatagttc agaggcaatg aaaagttatg tcagtgagca
     5341 aatcaagaat aaacaagaac aagctgcata cattaaacaa ttagaacgta gtttagaaga
     5401 aaataaaatc gaagataatg cggttcatag cgaactagat tctgttgaag aaaaaatagt
     5461 tcctgaagag acttttgaaa agaaaaaaga atactcacaa caagtcattg aattaaacga
     5521 aaaagagaac ttgtatagca aattgaaaga acgtttcgaa attgagcaac aagaaaaaca
     5581 aaaacgtcaa aaattgttac gaacaacgtt tattctttta acattagtag gtattggttt
     5641 aactgccttc tcatttattt caaacaacat gttgtttggt attattttcg cagtattaac
     5701 tttagtattt gttatcggta tcatcatgtc taaatcaaaa gaagtagatt atagcgaggc
     5761 catcacagat gaaatcgaag aaattaaagc acaactagcc attttagatg aaaattatga
     5821 cttagatttt gatcttgatg agcaatatcg cattcgtgat cactggcaac aagctttgaa
     5881 aaataaagat attttagaag agaaacgtca gtatattgaa ggtcgtttaa atgatgcgaa
     5941 agggcgtcat gatgaattac aatcgacagt tgagaacgtt aaggatgaat tatacttatc
     6001 atctaaaatt tcaaatgatt taattgttga tagtatttct acaatggcga atattaaagc
     6061 attagatcaa catatcagtg atttaaatca acaacgccag caattggttc aagaattaga
     6121 tacattctac aatcatgctg aagctgttac aaaatcacaa tttgtttatt tcaacaaatt
     6181 atctttattc cacgatgtgc aacaatggtt gaaatcagct gaagacacga atgaaaaatg
     6241 gcgtattaat gctgaaaata ccaaacttgt tacaaatgaa ttaaatcatt taaatgctca
     6301 attagaagaa aataataaag aaattacagc actattcgat tttatcaatg ttggtactga
     6361 agaagatttt taccaacatc atgaagatta ccaaacttat actagtaatt tgagtcgttt
     6421 taatgattta actaaatatc ttgagaacca aaactattct tatgaattaa gttctagttt
     6481 aagtgaaaaa acaactgcac aacttgaaga agaagatcat ttattggcta ctcaagttga
     6541 cgaatataat gagcaatatc ttgaaatgca agcacaagtc agtgatttaa gtgcacaaat
     6601 caatcacatg gaaactgata caacgcttgc taatttaaga catgaatatc acagtcttaa
     6661 aaatcaactt aatgatatcg caaaagattg ggcaagttta agttatttac aaagtttagt
     6721 tgatgaacac attaaacaaa ttaaagataa acgtttgcct caagttatta atgaagcggt
     6781 agaaatattg aagcatttaa cagatggcag atatacgatg attaactata atgaagattc
     6841 aattacggtt aaacatgtta atggtcaatt atatgatcct gttgaactaa gtcaatctac
     6901 aaaagaatta ctttatgtag ctttacgtat cagtttaatt aaggtactaa gaccatatta
     6961 tccgttccca ttaattgttg atgatgcatt tgttcatttt gataaaaaac gtactgaaaa
     7021 aatgttgaat tatttacgat cattatcaga acactatcaa gtactatact ttacatgtgt
     7081 aaaagataat attgttccat caaaagaagt gattacatta aacaaaatag aggaaggcgg
     7141 gaaacgatga gaaatataga gaatctaaat cccggagatt cagttgatca ctttttctta
     7201 gtgcataaag ctacacaggg tgtaacagca caaggtaaag attatatgac attacatttg
     7261 caagataaaa gtggtgaaat tgaagcgaaa ttttggacgg ctacaaaaaa tgatatggca
     7321 acaatcaagc ctgaagaaat tgtacatgtt aaaggtgaca tcataaacta tcgcggaaat
     7381 aaacagatga aagtcaacca aattagacta gcgacaactg aagatcaatt aaaaacagaa
     7441 caatttgtag atggtgcacc tttatcaccg gcagaaatac aagaagagat ttctcattat
     7501 ttgctagata ttgaaaatgc taatttacaa cgtatcacac gtcatttatt gaaaaaatat
     7561 caagaacgat tttacacata tccagctgct agttctcatc atcataactt tgcgagtggc
     7621 ttaagctatc atgtattaac gatgttacgt attgcaaaat caatttgtga catttatcca
     7681 ttgttaaaca aaagtttgtt atatagtggt attattttgc atgatattgg taaagttaga
     7741 gaattgagtg gtcctgttgc gacgtcgtat acagtcgaag gtaacttatt aggacacatc
     7801 tcgattgcga gtgatgaagt agttgaagca gctcgtgaat tgaacattga aggagaagaa
     7861 atcatgttgt taagacatat gattttatct catcatggta agttagagta tggttctcca
     7921 aaactgccat acttaaaaga agcagaaatt ttatgctata tcgataatat cgatgctaga
     7981 atgaatatgt ttgaaaaggc atataaaaaa actgacaagg gtcagtttac agataaaata
     8041 tttggtcttg aaaatcgtag attctacaat cctgaatcac tcgattaaga attaaaagat
     8101 atcggacaga tgagtatact gaaacatgag ctcatctgtc cgatttttaa tgcgaaaaaa
     8161 ccgagcggcc gtggtatgat ttttgaacca cggtcgctcg gttttgtgtt attggctcat
     8221 gccggattgt ccgccttgtg cgccaccttg tttaagtttt tcagggttta agattttatc
     8281 ttcgacaact gatttaatat cacgatcttt aaagtcaaca tcgtattctt ttaatagatc
     8341 tttgtatgca tcagtcaata attttggatt tttttgtact ttctgatcga ctaatttttc
     8401 tttcaggctt tgtttttcac tgttaaagtc tgttggttta tcagctttaa taatatgata
     8461 tccaaagctt gatttaacaa cctctgatac ttcaccatct ttaagcttaa atagtgcttt
     8521 ttcaaaatct ttatcagttt gtcctttaag aacataacct aattcgccat cttttttagc
     8581 tgaaccagta tccattgatt cttttttagc gatttcacca aatttacttg gatcttttga
     8641 aacttctttt tgaatttctt cagctttttg tttcgcttct ttatcatcta agccttcttt
     8701 gtcgcttttc ttagatttaa ctttaattaa aatgtgtgaa gctttcttgc tgtcttcttt
     8761 aatttcagaa tcagagattt taattttatc tgatagtaat tctttatgat aagcagcagt
     8821 acgtaaattt tctttatatt tatcggctgt taaaccttgc tgttgaaggg ccttttcaaa
     8881 tttatcttta ccgccgtatt gcttttgcat tttttcaatt tgttcgtcaa tcttcttatc
     8941 attaacttta tttttatatt tatcagctaa aattttattt aacatttcag taaatgatgc
     9001 atttgcaatt tgatctttac cgattttttt cattgtatct gcaactgtta cgtctccagc
     9061 tttagaagaa attaatgtat tttctttaga gtctgtggca ctagcgccac aagcgcctaa
     9121 taataaagca ctagctgtta ccggaacgat taatttgttt atcatcttca tagttgaaac
     9181 tcctttgtaa gttaatcatc gtgaatataa catagtttta tattatcacc aatcaataac
     9241 ccatgacatc agactagagc attaggttta ggggtagaaa ttgatgaaaa taaaaaaaca
     9301 aggtctatat acatttgatt gtaagctatt acatagccta aaaacaaata ataacacctt
     9361 gctataatta ttttgttata aatgatatgg atttttaatt tttttcaaaa tattaatacc
     9421 tgtactatgt tcttctatat attgtccata attagaaaat ataattattt ttaaatataa
     9481 taaatctgta atggaaatac cgaaattcaa tgccaataag aacataaaat aatgtccata
     9541 ttgcgggaat gaaattgtta gataaacgaa cattgcagat aagataatta acggcaatat
     9601 taagttaatg caaaaataaa atttgtgtac aggttgattg acgtaggtat tatagaatat
     9661 aagccatttt cttttagtta acttatgaac tttaaacgat tttctatatg gtaagaaaaa
     9721 taataaatgt atacttttat gtaatggata cgtcataaat acaagtaata aaaagatgag
     9781 aaaatgtcta tctgataatg gcgtattaga taaaaaatac attacttcat aactaactaa
     9841 aaacataatg atggttgcaa ctgcactcat aaatgcaatt ctaggcaaac caaatcgtgc
     9901 attgatatca atttgtcttt tacataaaaa catatcttac gcctcatctc ttttcaatat
     9961 aagttagatt ataactaact caggttcagt aattcaatag taacatatca tgttagaaga
    10021 attgttatat aagaagaaag gagcgacgta ttgtttgatt tgaaacgcga aagtggggat
    10081 tggtaaaatt gtctgattga ctagcgtaga agtagaatgc agaacaaatt tctgcgaaca
    10141 catattcttg ctaataaagc atcacttatg aaaaaggcgc gtgtgacatg agtatttgaa
    10201 aacttgaata atataaaaaa tggatatggc agggcatatg caaaagcatc ttttgaccaa
    10261 ctaagttata caaatgctaa aaatatagtg atatgtaatt ataataaaca ccagattaat
    10321 aatttgtgca agtaaggtat atgtaatgtg tatataaggt tcggctcaat gaaaatataa
    10381 gtatgttgtt tgtcgataat gatttaagta aagcgtaatc aaagtgcaag gataacacaa
    10441 tgaatcctaa gcaatgtttt caatcaattc ataaatatga tttaagcgaa aatcactaca
    10501 aacaatgtct tcctataatt tgtaaacata agttcatttg agttttaaaa aagagatgac
    10561 aagaaatatc taatttctaa tgtcatctcg tttatttggc atttaatttt aaaaaataaa
    10621 tatgctgtta ctaataaaaa tagttgtgat cgagaacgta acctgctact tagaaatttc
    10681 gtttccaata tcctcgccac gattttgtaa attttcgatg tgtgactgta aacgttcaat
    10741 attaggatta atgtcagatt tgaagtttcc aagtaatgac ttaatttcac caccaattga
    10801 actaccaaat tccgcacttt cgtttttaat ttgaacacca tagtttaaaa tgtcattaaa
    10861 actttgttta atcgtttcaa attcacgttg tagttctgat tttgaaccag taggggcagt
    10921 acgatcgatc gtgttgttct tgacactttt gtcgtcacgt ccttgaagtg caactacaaa
    10981 accagctgct acgccaacac cgataccgaa tagaatgcgt gatgctttca tttatattct
    11041 cctcactttt aacatctgtt atcttgataa ctcattttgt ttaaaattgc taaccttgat
    11101 gttatcactt caaacgcaat tgttataata ttaatcatat tatacccgtt cgagcttaga
    11161 ttaagcatac atttaaaatt gtgcttgaat ttgttcagca atttgttgtt ttgcatcgtt
    11221 atctaaaatg tcttcatgtg tttcccactt ataaccaaat ccatcaatat cattttcgta
    11281 tcgaggaatt aagtggaaat gaatatgaaa tacagattga tctgcaaact caccattatt
    11341 ttgaataatg tttaaaccat caggattaaa tgcacgctta atagcatttg ctactttagg
    11401 taatgctgca ccgatatgtt tcattgtttc ttcatcagtt tcaaagatat tagcagaagc
    11461 ttttttagga attaataacg tatgtccttt agtaacttgt gatatatcta aaaaggcata
    11521 gacataatcg tcttcatata ctttaaagct aggaatttct ccagttaaaa ttttgccgaa
    11581 aattgtttct gacatgatga ctacactcct tttcaactag tattaccatt cattataaca
    11641 ttgtcgctaa aaattcgata ttaggacatg tgtatttaat atgagcattt tataattttt
    11701 ggtacaatat aagtcatgga ggtgccttat gacagttaaa gtagaacagc ttacaggtgg
    11761 atatggaaaa cgccctgtaa ttaaagatat aaattttgaa ttaaacaaag gcgaaattgt
    11821 tggacttatt ggccttaatg gcgctggtaa gagtacaacg attaaacata tgctaggatt
    11881 gcttacacct atggaaggat ctctatcaat ttcagatatc aacattaatg atgacataga
    11941 ggcttataga agaaagttat cttatattcc ggaatcaccg gttatttatg aagaactcac
    12001 attagaggaa cacattgaga tgacagcaat ggcatatgat attgatcgtg atgaaacgat
    12061 gaatcgagca atgccattat taaagacatt ccgtttagaa aatgagttga aagtttttcc
    12121 gagtcatttt tctaagggga tgaaacaaaa agtcatgatt atttgtgcgt ttatcgttaa
    12181 tcccgaattg tatattatag acgaaccatt ccttggccta gatccattag gaattcaatc
    12241 catgttagat ttaatggttg aaaagaaaaa cgaaggtaga accgttctaa tgagtacaca
    12301 tattttagca acagctgaac gttactgtga tcgttttatc atactagacg aaggtgaagt
    12361 cgttgcattt ggagatttag aagcattgag acaacaaact ggcttacata atcaaacatt
    12421 agacgatata tatattcatg tgacgcaagg tggagatgta catgcgtaac caagctacca
    12481 cattatttaa taaaagatta catgcccttc gtaaagagaa aaattattat aataagttta
    12541 tatttaatgg tcatttcatg gtctttttac ttattttgct gggtgcattt atttttggat
    12601 atggtgaatg gttaaaacat attccaacga atatcaattt ttcattaata gcagcagtga
    12661 ttgttgcgtt gacatctatt tttccaatgc gaccactgtt aaaagaagcg gataaaatct
    12721 ttttattacc atttgaaaaa cacatgtctc aatttatgcg tcatgcgatt ttatatagtt
    12781 actttgcgcg catattaatc caactcatta ttgttattgt aatgttcccg ttgttttaca
    12841 atattaatca acataatgtg gcattttata tttgttttgg agtcagtgca ttaatttttc
    12901 catatgttgg tttacgctta agatggcaat ggtatcaatc gggacttaaa acatggcaag
    12961 ttaatttaat atcattcatc acctttgctc taacatatta cttactatta gctccaaaat
    13021 ggtacatcgc atttgtgatg gttgctttgc ctgtgttaat agaattttta gttaaaaaat
    13081 ataaaccagg ctttttgtat ccttgggaga aaatgattgc aatcgaacat cgacatcata
    13141 tgaattatta taaatttgtt aatatgttca ccgatgtaaa acatttgaaa gaatctgcag
    13201 tcagaagaag ttatctagat atacttttac cagttccaaa gggtagtaaa tttaactcta
    13261 atgcgatgta tttattctta tttattagaa gctttataag agggagagac gcatttaata
    13321 ttatctttag gttagtaatt attgcagttt tattaatggt ttggttatca tatccattag
    13381 tgacagcaat tattgggtgt ttatttgtat atattatatt gttacaaatg gcacaatttt
    13441 attcacagca agcatatggc ttatggcctc aagtgtggcc tgtacctgaa gagaaggtaa
    13501 tcaaagggta tgaacagttt ttatatagat taatgtttgt tatttgtact gtgtttgcag
    13561 tgacatttat tataaaacat atgacgttat tctatgttgt actcattttc tatattgtag
    13621 gtttattaac gattagaagt attattaaaa agttgaaata tcaggaaaca ttattacgag
    13681 actaaaaaac ttccattggc atgtatgttg taaaggtgca tgtaatgttg aacgccaaat
    13741 gatacggcgt tcagattaca ttagcatcta tacgttaaca gcataaccaa tggaagtttt
    13801 cttcgaatct attcttttat tgggtataga tatctttcaa aataacttga atgttgtccg
    13861 cttgaaccaa agtaatgact taatgcgtct tttgaaaaat ggtcattaaa ggcatcagat
    13921 tgcttaaagt cttcgtatgc atgtcgatca gcaaatccga aataaatttt gtatgttgta
    13981 cctttagcag gtcttaacaa acgatagctt ttaaagccac caaagtttct gaaattatcg
    14041 tctacactaa tcagtttctt ttcaagttga tatgcatgat cttctgttga tggaatgaag
    14101 attgcacaat agaaatgatg ttcactgaat tcaccaattt ctttaataac ttcatatatt
    14161 gaaggtgatt ttaaaacagt ctcaccatca gtttcttcaa aaataactga agtatctgat
    14221 gctgaaaatt ggaatagttg atgggtcggg ttattgattt ttatctgatg taaaaatcca
    14281 taagtgccat aagatgtata tagtttcttc atatgaaagg tcccccttat gtttttattt
    14341 attatacgat gataaactag tcattaccac tattaataat tgattaaaat tactataatg
    14401 aatcttgcga gatatttaga agtaatgata caaaataatg gactttagaa tttaaattta
    14461 caataaatag ttctatgatt aaatgtcagt tttatgacat ttatttattg aaaatacgaa
    14521 cgaatgagcg atatgataat atagataaga atgattttaa tttaggaggc ctttatggtg
    14581 cataataaaa acaatacaat tttaaaaatg atcaaaggtg aagaaacatc acatacacct
    14641 gtttggttta tgcgacaagc tggccgttcg caaccagaat atcgaaaatt gaaagaaaaa
    14701 tattcactat tcgatattac acatcagccg gagttgtgcg cttatgtaac acatttacca
    14761 gttgataatt atcatacaga tgcagcaatt ttatacaaag atattatgac accattaaag
    14821 ccaattggtg tcgatgtaga aattaaatcg ggtattggtc cagtgattca taatccaatc
    14881 aaaacaattc aagatgttga gaaactttct caaatagacc ccgaacgaga tgtaccatat
    14941 gtattagata caattaaact tttaacagaa gaaaagttaa atgtgccgct aataggattt
    15001 actggggcac catttacatt agcgtcatat atgattgaag gcggaccatc gaaaaattac
    15061 aattttacaa aagcgatgat gtatagagat gaagcaacat ggtttgcttt aatgaatcat
    15121 ttagttgatg tatctgttaa atatgtaaca gctcaagtcg aagcaggtgc cgaattgatt
    15181 caaattttcg attcatgggt aggtgcatta aatgtcgagg attacagacg ttacattaaa
    15241 ccacatatga ttcgattaat cagtgaggtt aaagaaaaac atgatgtgcc ggtaatttta
    15301 ttcggtgtag gtgccagtca tttaatcaat gaatggaatg atttaccgat tgatgtatta
    15361 ggcctagatt ggagaacgtc tattaatcag gctcaacaat taggcgttac taaaacatta
    15421 caagggaatt tagatccatc aattttatta gcaccatgga atgtcattga agagagattg
    15481 aaaccaatat tagatcaagg tatggagaac ggtaaacaca tctttaattt aggacacggt
    15541 gtattcccag aagtgcaacc agagacgtta cgtaaagtaa gcgaattcgt acacacatat
    15601 acacaaagat aatcatagtt ttacattgaa gcacatgaaa gataattcat ttaaaggaag
    15661 gtattatcaa tgactaaaaa aatgggatta ttagttatgg cttatggcac accttataaa
    15721 gaaagtgaca tagagccata ttatacagat attagacatg gtaaacgtcc atctgaagaa
    15781 gaacttcaag atttgaaaga tagatatgaa tttataggtg gtttatcacc attagcaggt
    15841 acaacagatg accaggctga tgcgctagtt tcagcattaa ataaagcata tgcagatgtt
    15901 gaatttaaac tatacttagg attaaaacac atttcaccat ttatcgaaga tgcggttgaa
    15961 caaatgcaca atgatggcat tactgaagca atcacggtag tactagcacc acattattct
    16021 tcattttcag taggatcata tgacaaacgt gctgatgaag aagctgcaaa atatggtatt
    16081 caacttacac atgtgaaaca ttattatgaa caacctaaat ttattgaata ttggacgaat
    16141 aaagtcaacg aaacattagc tcaaataccg gaagaggaac ataaagacac ggtattagtt
    16201 gtttcggcac atagtttgcc aaaaggttta atcgaaaaga ataatgatcc atatccacaa
    16261 gaactagaac atactgcgct tttaattaaa gaacaatcta atattgaaca tatcgcgatt
    16321 ggttggcaat ctgaaggtaa tacaggtaca ccttggttag ggccagatgt acaagattta
    16381 acacgtgatt tatatgaaaa acatcagtat aaaaacttta tatatacgcc agtaggtttt
    16441 gtatgtgagc atttagaggt gctttatgac aatgattatg aatgtaaagt agtttgcgat
    16501 gatattggtg cgaattatta tcgtccaaaa atgccgaata cacatccatt atttatcggt
    16561 gcaattgttg atgaaatcaa gtctatattt taatgacgaa agaagcgtga aacgttgtga
    16621 ctaaatcagt ggctattata ggagcgggga taacaggttt atcaagtgca tattttttaa
    16681 aacagcaaga tcctaatatt gatgtaacca tctttgaagc atcgaatcgt ccggggggaa
    16741 agattcaatc gtatcgtaaa gatggttata tgattgaact agggcctgaa tcttatttag
    16801 gtagaaaaac gattatgaca gaattagcga aagatattgg attagaacaa gatattgtta
    16861 caaatacgac tggacaatca tatatttttg cgaaaaataa attatatccg attccaggtg
    16921 gttcaattat gggtattcca acagatatta aaccatttgt tactacaaaa ttaatatcgc
    16981 cacttggtaa attaagagca gggtttgatt tattaaaaaa acctactcaa atgcaggatg
    17041 gtgacatttc tgttggtgca tttttcagag caagattagg taatgaggta cttgagaatt
    17101 taatagagcc tttaatgggt ggtatttatg gtaccgatat tgataaatta agtttgatga
    17161 gtacgtttcc taattttaaa gaaaaagaag aggcattcgg aagtctgata aaaggtatga
    17221 aggatgagaa aaataagcgt ctgaaacaaa gacaattata tcctggcgca ccgaaaggac
    17281 aattcaaaca atttaagcat ggtttaagct catttattga agcattagaa caagatgtta
    17341 aaaataaagg tgtgacaata cgctacaata cgtcagtgga tgatataatt acatctcaaa
    17401 aacaatataa aattgtttac aatgatcaac tagaagaagt gtatgatggc gtattagtaa
    17461 cgacaccaca tcaagtgttt ttaaattggt tcggacaaga tccagcattt gattacttta
    17521 aaacgatgga tagtacgact gttgcaactg ttgtattggc atttgatgaa aaagacattg
    17581 aaaataccca tgatggtact ggcttcgtaa ttgcgagaac gagtgataca gacattaccg
    17641 catgtacttg gacatcgaaa aaatggccat ttactacacc ggaaggtaag gttttgattc
    17701 gtgcgtatgt aggtaaacca ggtgatactg tggttgatga tcatacagat aatgaattag
    17761 tatcgattgt acgtagagat ttaagtcaaa tgatgacatt taaaggtgat cctgaattta
    17821 caattgtcaa tcgtttgccg aaaagtatgc cacagtacca tgtcggtcat attcaacaaa
    17881 ttagacagat tcaagcacat attaaacaaa catatccacg acttagagta actggtgcat
    17941 cttttgaagc ggttggacta cctgattgta ttacgcaagg taaagttgct gctgaagaag
    18001 taatcgcaga gttgtaagtt aattgttgga tggtgttggt gtaggtgcga cgcctttaga
    18061 taatgtttga tgaaatatag tcatttttaa gttatataat ggtggaagta atgtgtaaat
    18121 atagatactt tcagcgggaa ttatttttgt gaaattgaag cagagacaaa ggtttagctg
    18181 tttttgaatg atattgaaat gatgacaata cactttaatt agaaatgtca ctttgcgata
    18241 taacatttct aattaaggtg tttttatttt acaattatag gtaatcagtc atttttttaa
    18301 aaatagaaaa gaagtagtaa gttgaaagga gagaatgaga tgacagatgt aattattgta
    18361 cattctatgc atggaaatag tagaaatcat tggtatcagt ggttggagca taacttaact
    18421 ttagaaggat atgacgtaac attatttaat tttgaatccc ctgaagcgaa gacagtggat
    18481 cagtggattg aagctatgac gaaacaaatt aatgtacgca aaaaggatac atattttgta
    18541 acacatggtt taggttcaat cacagcttta aaatatattg aaatgattga tcagccgatt
    18601 gaaggctttt tcagtatagc gggctttaaa gaagatgcag aaaatataga tttagatatt
    18661 gatttaagta atgtaaccat tgattacgat aatattaaaa agaaagttga caatttttac
    18721 ggattgagtt ctaaaaatga caaatacgtt tcatataaag agacccagcg tttaatgaat
    18781 gcattagagg ggaacttgcg aatcgtggaa gatggagggc acttcttaga agaggatggc
    18841 ttcgaaacat ttacagcgtt gcaagatcgg atgcaagatt acatgacacg atagttagtg
    18901 tatgggttgt gttatgttgt gtcagtaggt agctgctgat tttgaaaaat tgttgtaatt
    18961 gaggcgtcaa gcaatgtctt agggctacaa tgttatattg gtagtcgctg actgaaagaa
    19021 tatgcggtgt aacaagcttt tataatttct ttttcagaag gtataggcgt tgtaaacttt
    19081 ataactccta taccttttta aatgctttaa cactatttat gacaactatt agacacttat
    19141 atgataaaag ggaataagaa gcgttttttt acacaaaaaa ctttgaaaat gtcgttgacg
    19201 tacactacaa aaatgaatat actaatattt gtgttcaaca gagaacatgt tgctacgata
    19261 tttaagtaat tatttaagag ataacattta aattaaattc aaaaagttct tgactttagg
    19321 tttttgatta tggtatatta attaggtaat tcaaaataat attagcgcgg gatagagcag
    19381 ttcggtagct cgtcgggctc ataacccgaa ggtcggtggt tcaaatccgc ctcccgcaat
    19441 acatagtttt taatttaata ggtctcgtag tgtagcggtt aacacgcctg cctgtcacgc
    19501 aggagatcgc gggttcgatt cccgtcgaga ccgccattat tattaccatt acggttcagt
    19561 agctcagttg gtagagcaat ggattgaagc tccatgtgtc ggcagttcga ctctgtcctg
    19621 aaccattctt aattcatggc gg
//
LOCUS       DALAGD010000059        19547 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.060, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000059 DALAGD010000000
VERSION     DALAGD010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19547
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.060"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            374..1246
                     /locus_tag="O2701_001688"
     CDS             374..1246
                     /locus_tag="O2701_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCZ4293576.1"
                     /translation="MSNVLTEIDSQYPYMTKNEKKIAKFILNSPQKVIKMRSQDLASL
                     LDISTSSVIRFSKKITDGGFHDLKINISKYVPKASSIYNVELMNNESTESLRTKLHTR
                     TTRALNHANNELNDKTIDQICHCLKRSETIFIYGFGASFVVATDLYQKLSRIGLNIQL
                     VQETHIFATLLATHNSNDSVILITNNGTQSEMQSMVKVIDDYHIPIITITSTRDNPVA
                     QASNIVLTYGKTDENEMHMGATTSLFAQMFTIDILYYRYVALNYHASLDFITQSKMAL
                     DNYRKHLSNINFKH"
     gene            complement(1633..3012)
                     /locus_tag="O2701_001689"
     CDS             complement(1633..3012)
                     /locus_tag="O2701_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCZ4293577.1"
                     /translation="MENNELQRGLSARQIQMIALGGTIGVGLFMGATSTIKWTGPSVI
                     LAYLIAGIFLFLIMRAMGEMIYLNPTTGSFATFASDYIHPAAGYMTAWSNIFQWIVVG
                     MSEVIAVGEYMKFWFPELPTWIPGVIAILLLMAANLFSVKAFGEFEFWFALIKVVTII
                     LMIIAGFGLIFFGFGNGGHAVGISNLWTNGGFMPNGIVGFFFALSIVIGSYQGVELIG
                     ITAGETKDPQKNIVKAVNGVIWRILIFYLGAIFVIVSVYPWNQLGDIGSPFVATFAKI
                     GITFAAGLINFVVLTAAMSGCNSGIFSASRMIYTLAHKGEMPKIFTKIMRNGVPLYTV
                     VAVSLGILIGALLNVILPLYIDGAKSIFVYVYSASILPGMIPWFMILFSHLRFRKLHP
                     EELEGHPFKMPGGAVTNYLTILFLILVLVGMVFNVETRISVLIGVIFLTIVTIYYFIR
                     YNKNNVKAK"
     gene            3292..3648
                     /locus_tag="O2701_001690"
     CDS             3292..3648
                     /locus_tag="O2701_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293578.1"
                     /translation="MLKQILLRATKISVLFAVAFFIINYIGMEKPDILYLVGRTIIAT
                     LAFILICLTVFTIINSPERKIKLGTTLPIALIIGIIFGAIFLTVQIGVITGLIIGVIA
                     TFIWELIEKNKGGRSS"
     gene            3645..3830
                     /locus_tag="O2701_001691"
     CDS             3645..3830
                     /locus_tag="O2701_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293579.1"
                     /translation="MTTALIGVIVLILLIISFVPNYRAMKSAKEQGSNATRATIMVGI
                     DIILIVLIIVTLLLKYM"
     gene            complement(3926..4117)
                     /locus_tag="O2701_001692"
     CDS             complement(3926..4117)
                     /locus_tag="O2701_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293580.1"
                     /translation="MMGYIILFFLAGPVILGVGNLVIGPIFNKQTPFRVQVRSFVVGS
                     MIYLILATIGYFLLLQGKL"
     gene            complement(4128..4763)
                     /locus_tag="O2701_001693"
     CDS             complement(4128..4763)
                     /locus_tag="O2701_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCZ4293581.1"
                     /translation="MYRAVIFDFDGTIIDTEQHLFNVINKHLEMHNADPISIDFYRSS
                     IGGAATDLHDHLIKAIGSENKDKLYEEHHLTSTTLPMIDTIKSLMAFLKQRHIPMAIA
                     TSSVKAEIMPTFKALGLDDYIEVVVGREDVEQVKPDPELYLSAVQQLNYMPTQCLAIE
                     DSVNGATAAIAAGLDVIVNTNKMTSAQDFSNVDYVAKDIDYDQIVARFFTK"
     gene            complement(4838..5755)
                     /locus_tag="O2701_001694"
     CDS             complement(4838..5755)
                     /locus_tag="O2701_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HCZ4293582.1"
                     /translation="MFQKLSIFATKSFLVWMLVAAVIGFIFPQHVATLGKWVPYLLGI
                     VMLGMGLTITPNDFKMVFKAPRAVIIGVCLQFSIMPTLAFIIAKSFHLPPDIAVGVIL
                     VGCCPGGTSSNVMSYLAKANVALSVSITTVSTLLAPFVTPALIYLFANEWLEVSFVSM
                     LWSVVQVVLIPIALGIVLQIINRKIAEKASTALPIISVVAISLILAIVVGGSKHQILT
                     TGLLIFLVVILHNVLGYTIGYWLARLLKLDRQDQKAVSIEVGMQNSGLAVSLAALHFN
                     PIAAVPGAVFSFIHNITGPILAKYWSKKL"
     gene            6023..6553
                     /locus_tag="O2701_001695"
     CDS             6023..6553
                     /locus_tag="O2701_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293583.1"
                     /translation="MLIDKFETYIINIAGLNDRTTRKKLSKLCKSVQFCDALQFSINK
                     QFNQYVLEISLPKQQLPYFISFLSFHQYSIFQVLSPKKINELLDSDNLYQSAKRFDIN
                     IDGLQDAFIKDKVIDIMNMFQNHTDITYTLNKSHAHIICTPEIFAKLLHTIATRNIDI
                     LSANYRSSSMSKARIS"
     gene            complement(6619..8223)
                     /locus_tag="O2701_001696"
     CDS             complement(6619..8223)
                     /locus_tag="O2701_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCZ4293584.1"
                     /translation="MNAIKRFGSAMIVPVLMFAFFGIVLGFATLFKNPTIMGSLADQH
                     TFWFKFWSVIESGGWVIFTHMEVVFVVGLPLSLAKKAPGHAALAALMGYLMFNTFINA
                     ILTQWPHTFGANLEKGVENVPGLKSIAGIATLDTNILGGIIISAIITWIHNRYYSKRL
                     PEMVGVFQGLTFVVTISFFVMLPLAAITCVIWPTVQHGIGSMQHFIIASGYIGVWLYH
                     FLERVLIPTGLHHFIYAPIEVGPVVVNHGLKAEWLQHLNEFAKSTKPLKEQFPYGFML
                     QGNGKVFGCLGIALAMYATTPKENRKKVAALLIPATLTAVVVGITEPLEFTFLFIAPY
                     LFVLHAVLAASMDTLMYAFGVVGNMGGGLLDFISTNWLPLGKEHWGTYVAQVIIGLIF
                     VAIYFFLFRFLILKFDIPLPGRKKTEEEVKLFSKQDYKNKKGDSVDSKRASSGNEYED
                     KAAYYLDGLGGKENIKDVTNCTTRLRLTVYDESKVADTEYFTHQQMAHGLVKSGKSIQ
                     VVVGMTVPQVREAFEQMVEDQSSEDK"
     gene            8553..9317
                     /locus_tag="O2701_001697"
     CDS             8553..9317
                     /locus_tag="O2701_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCZ4293585.1"
                     /translation="MFLDEHINRNFDKLNDNDLHIAHFINTHIDECKNMKIQDLAQFT
                     HASNATIHRFTRKLGFDGYSDFKSYLKFESNKTHQLPSNSIDSFKQEIDSTFSYLERV
                     DYQLLTKKMHTASTIYLYGTGRAQLNVASEAQRILLTLHNHVIVLHDEHELKMILNKS
                     EPNDLFFIISLSGETHELLEVTNLLQLRQKYFISVTTMKDNTLAQKANYNVYVSSNTF
                     YLTDGTDYSSFISYHIFFETLVRKYNEYLLHNDISN"
     gene            complement(9375..9881)
                     /locus_tag="O2701_001698"
     CDS             complement(9375..9881)
                     /locus_tag="O2701_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HCZ4293586.1"
                     /translation="MAKFNVENEHVEVEIEKLYKFSPELVYEAWTKKDLLKQWFMTSA
                     RTNKEIEADVKEGGKYRIVDQQRNGKVNVIEGIYESLVMDEYVKMTIGMPGLSETQDV
                     IEVEFFERETGGTQMLFYYRSLVEKERRFTNLEYKQKKKEYHDAMVHGFELMFDKMYH
                     VIETSTQQ"
     gene            complement(10048..11361)
                     /locus_tag="O2701_001699"
     CDS             complement(10048..11361)
                     /locus_tag="O2701_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HCZ4293587.1"
                     /translation="MEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLNVAITIT
                     VIVALLMNRKESFAKKVEVFTKGAGHSNIVLMMLIFILAGAFSNTAEKMGGVKSTVNL
                     GLSLIPENLIIVGLFVICMFVSISMGTSVGTVAAIAPVGYGFAQATDVPTALAMATVV
                     GGAMFGDNLSMISDTTIAAVRTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISL
                     NHAKNYDYNLIKVVPYILVLVLALLGVNVIIVLIGGTLLAGIIGLIDGSFGWMGLLDA
                     VSKGIISMEDIAMIALLIGGLVGIIQHNGGIEWLLQFVRSKVKSKRGAELGIASLVSV
                     ADIATANNTISIIMSGPLAKNIADEYDVDSRKSASILDIFGGCFQGLLPYSPQVISAA
                     GVAGISPLMLFPYSIYPILLGVCGLIAILFNFPKLKKNTSQEMKH"
     regulatory      complement(11438..11533)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(11586..12155)
                     /locus_tag="O2701_001700"
     CDS             complement(11586..12155)
                     /locus_tag="O2701_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293588.1"
                     /translation="MALIVSRAILFLILALAFGIATVFPEQKLPLYIVFTLFVAVAIV
                     SNVWFTHQNVKKHIIKFNKYVAYFLSFVSGLLYIGFVIYTLTIGSYDVLPIIIFAISI
                     VLVQILENILGISIKNTRLSNKQLKVISKQRVQQFKVISLILVSVSIVFIFIALMLKW
                     WLLLVISIIVLIIITVAMIVYEKYYTTPD"
     gene            complement(12642..13523)
                     /locus_tag="O2701_001701"
     CDS             complement(12642..13523)
                     /locus_tag="O2701_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCZ4293589.1"
                     /translation="MAAQDPRTKFKTTDYEKQEQEVPGLQSEMTPAPDCGETSYQGHQ
                     RLQGYKILVTGGDSAIGRAAAIAYAKEGADVAINYLPSEEQDAQEVRQVIEESGQKAV
                     LIPGDIRDEQFNYDLVEQAYQQLGGLDNVTLVAGHQQYHDDIHGFTTEAFTETFETNV
                     YPLFWTVQKALEYLKPGASITTTSSVQGYNPSPILHDYAASKAAIISLTKSFSEELGP
                     KGIRVNCVAPGPFWSPLQISGGQPQSKIPTFGQKTPLGRAGQPVELCGTYVLLASEES
                     SYTTGQVFGVSGGVQID"
     gene            complement(13634..14755)
                     /locus_tag="O2701_001702"
     CDS             complement(13634..14755)
                     /locus_tag="O2701_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HCZ4293590.1"
                     /translation="MGLKDQAVTWRRYFHQFPELSDKEFKTTQKIKDILTEHHIRILD
                     LPLATGLVAEVGQGLSCIAVRADIDALPIQELVEQDFKSENEGVMHACGHDIHMASIL
                     ATAVKLKEIEGTLTGRVKFIFQSAEELGHGAFKIIETHALKDVQAVLGFHNDPSRSVG
                     TFAIKTGAITSAVDRFEFHIKGVGGHAAKPEQCNDPVIVLAQLINSIQSIVSRNLSAF
                     DEAVVTIGQISCGNTWNVIADHAYVQGTVRSFDPVVRKLVETRLQDIADGLAQAYNMK
                     INLNYTHLPGAVMNDEALTHKAIAVAQHVGYKVEMMEQPLTIGEDFSGYSQHFPSVFA
                     LIGSHSEYDLHHPQYKPDERILEKVPEYFVEFVKRLLHE"
     gene            complement(15032..16270)
                     /locus_tag="O2701_001703"
     CDS             complement(15032..16270)
                     /locus_tag="O2701_001703"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HCZ4293591.1"
                     /translation="MNDLIINHIAELILPRSTDKPLKGKELDELNVVKNGTVVIKDGK
                     IVYAGTHTDDYDATETIDASGKVVSPALVDAHTHLTFGGSREHEMSLKRQGKSYLEIL
                     EMGGGILSTVNATRETSEDDLFKKAEHDLLTMIKHGVLAVESKSGYGLDRENELKQLK
                     VSNRLAEKYDLDMKHTFLGPHAVPKEASSNEAFLEEMIALLPEVKQYADFADIFCETG
                     VFTIEQSQHYMQKAKEAGFKVKIHADEIDPLGGLELAIDEQAISADHLVASSDKGKEK
                     LRNSDTVAVLLPATTFYLGKEDYADARGMLDNNGAIALATDYNPGSSVTNNLQLVMAI
                     AALKLKLSPNEVWNAVTVNAAKAIDINAGTINTGDKANLVIWDAPNHEYIPYHFGINH
                     AEKVIKDGKVIVDNTVSFKA"
     gene            complement(16270..17931)
                     /gene="hutU"
                     /locus_tag="O2701_001704"
     CDS             complement(16270..17931)
                     /gene="hutU"
                     /locus_tag="O2701_001704"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HCZ4293592.1"
                     /translation="MRKIQAKKGLSIECKGWEQEAVLRMLYNNLDPEVAERPEDLVVY
                     GGIGKAARNWEAFEAIEKTLRELESDETMLVQSGKPVAVFKTHEEAPRVLISNSVLVP
                     EWANWDHFNELDKKGLIMYGQMTAGSWIYIGSQGIVQGTYETFAELGNQHFNGDLAGT
                     VTLTAGLGGMGGAQPLAITMNHGVAICVDVDETRVDKRIDTKYCDVKTADLDEALKLA
                     EEAKERGEGLSIGLVGNAVDIHQAILEKGFKIDIITDQTSAHDPLNGYVPQGYSVEEA
                     KVLREKDPKKYVELSQASMAKHVELMLEFQKRGAVAFDYGNNIRQVAFNNGVKNAFDF
                     PGFVPAYIRPLFCEGKGPFRFAALSGDPKDIERADEEMRKLFPENEKLLRWLDLAEEK
                     ISYQGLPSRIAWLGYGERAKMGLALNRLVRDGEISAPIVIGRDHLDAGSVASPNRETE
                     SMKDGSDAVGDWAVLNALINTAAGGSWISFHHGGGVGMGYSLHAGMVVVADGSERAER
                     RLERVLTTDPGMGVARHVDAGYDIAIQTAKEKGIHIPMIDKAGDK"
     gene            18091..18975
                     /locus_tag="O2701_001705"
     CDS             18091..18975
                     /locus_tag="O2701_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCZ4293593.1"
                     /translation="MKIIQLEYFLAIVKYNSFTKAAQFLHISQPSLTATIKKMEADLG
                     YDLFTRSTKDIKITEKGIQFYRYASELVQQYRSTMEKMYDLSVTSEPRIKIGTLESTN
                     QWIANLIRKHHSDYPEQQYRLYEIHDKHQSIEQLLNFNIHLAITNEKITHEDIRSIPL
                     YEESYILLAPKETFKNQNWVDVENLPLILPNKNSQVRKHLDDYFNRRNIRPNVVVETD
                     RFESAVGFVHLGLGYAIIPRFYYQSFHTSNLEYKKIRPNLGRKIYINYHKKRKHSEQV
                     HTFVQQCQDYLYGLLEAL"
     gene            19097..19516
                     /locus_tag="O2701_001706"
     CDS             19097..19516
                     /locus_tag="O2701_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosB1/FosB3 family fosfomycin resistance
                     bacillithiol transferase"
                     /protein_id="HCZ4293594.1"
                     /translation="MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFELAG
                     LWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQ
                     SIYFTDPDGHKLELHTGTLENRLNYYKEAKPHMTFYK"
BASE COUNT         6558 a         3806 c         2741 g         6442 t
ORIGIN      
        1 taaaaagttt cttcaaattg taaagatgca ttaatcgagt tataatcata atgattaaga
       61 tggtagattt agtcttatat aactacattt actaagattt aaatagatga gtacataatg
      121 tataaattgg gaatatacag tatgacttac ggtaatatat caatatattg atatattatt
      181 tgcacatggg agtatgctat tttttatcta tacatcttaa aaaaatttac agttttacaa
      241 ttcaatatca ttaaacaaac aacatattca gcttattttt cattctctta cgttacaagt
      301 tgtaaatacc atcgcgttaa acaacaatag ccaacgctaa taaaatatat tgcgttaaag
      361 tggagtgatt aatatgtcaa acgtactaac agaaatagat agtcaatatc catatatgac
      421 taaaaatgaa aaaaagatag ctaaatttat actaaattca cctcaaaagg tgattaaaat
      481 gaggtctcaa gacttagcaa gcttattaga tattagtaca tcatcagtca ttcgattcag
      541 caagaaaatt actgatggcg gttttcacga cttgaaaata aatatatcta aatatgttcc
      601 taaagcatca tcaatttaca atgttgaatt aatgaataac gaaagcacag aatctttaag
      661 aactaaactc catacacgta ctacacgcgc acttaatcat gcaaataacg aattaaatga
      721 taaaactatt gatcaaatat gtcattgttt aaaacgttct gagacgattt ttatatatgg
      781 ttttggcgca tcttttgtag ttgctaccga cttataccaa aagttatcaa gaataggtct
      841 taatattcaa cttgttcaag aaacacatat ttttgctaca ttattagcaa ctcacaattc
      901 gaacgacagt gttattctta ttactaacaa tggtacgcaa agtgaaatgc aatcaatggt
      961 taaagttatt gatgactacc atatacctat aattacaatt actagtacaa gggataatcc
     1021 cgtagcacag gcatcaaata ttgttttaac ttatgggaaa actgatgaaa atgagatgca
     1081 tatgggagca acaacttcat tatttgctca aatgttcact attgatattt tatattatcg
     1141 ttatgttgca ttaaattatc acgcatcatt agatttcata acacaatcta aaatggcttt
     1201 agataactat cgtaaacatt tatcaaatat taactttaaa cattaacagt cttatataaa
     1261 ttctttaatc atgtcatata ataatttatg catcattttc aaagcttaca tgtcttttat
     1321 cttgtataac atacgcatat gaccaatgat ttgataaggc cattcaattt cttatattaa
     1381 aaacaaacgc agttgattaa cggattcatt ccatatatca attgcgtttg ttattttaat
     1441 aatgctattt tttattttaa agcccacagt aaaaactaac gataatattg ccacttataa
     1501 gtttttcaca ctacaacttc gatttaagct atatttataa aaaaatgcgc ctactcataa
     1561 cattctttgg ctatttaact ttaataagct agggccaagt ttgtcataag ataagcgcac
     1621 attataatca tactattttg cttttacatt atttttatta tatctaataa aataataaat
     1681 cgttacaatc gttaagaaga tgacaccaat aagcactgat attctagttt caacattaaa
     1741 taccatacca accaatacta atattaagaa taagattgtt aaatagttag ttactgcgcc
     1801 accaggcatt ttgaatgggt gaccttccaa ttcttcaggg tgtaattttc taaaacgtaa
     1861 atgactgaat aaaatcataa accaaggaat catacctggt aaaatagacg cactataaac
     1921 gtatacgaaa atactcttcg ctccatcgat atataaaggt aaaattacgt ttaataaagc
     1981 accaattaaa ataccaagag atactgctac aacagtgtac aatggtacac catttctcat
     2041 gattttagta aatattttcg gcatttcacc tttatgagca agtgtataaa tcatacgact
     2101 cgcactgaaa atacctgagt tacatcctga cattgctgca gttaatacaa caaagttaat
     2161 taatccagct gcaaatgtaa taccgatttt agcaaatgtt gcaacaaacg gacttccaat
     2221 gtctcctaat tgattccaag gatacactga aacaataaca aaaatagcgc ctaaatagaa
     2281 aattaaaatt ctccagataa caccattcac tgctttaacg atatttttct gaggatcttt
     2341 agtttcacct gccgtaatac ctatcagttc cacaccttgg tatgaaccaa ttacaattga
     2401 taatgcaaag aagaaaccaa caataccatt tggcataaat ccgccatttg tccatagatt
     2461 agaaatacct accgcatggc caccatttcc aaaaccaaag aaaataagac caaaaccagc
     2521 aataatcatt aaaataattg ttacaacttt aattaaagca aaccaaaatt caaattctcc
     2581 aaacgctttt accgagaata aattcgctgc cattaataat aaaatagcaa taacaccagg
     2641 aatccaagtt ggcaattccg ggaaccaaaa cttcatatat tctcctactg cgatgacctc
     2701 actcatacca actacaatcc attggaatat attactccat gctgtcatat aacctgctgc
     2761 aggatgtata taatcacttg caaatgttgc aaatgatcct gttgtagggt ttaaataaat
     2821 catttccccc attgctctca tgattaaaaa taaaaagata cccgcaatta aatatgcaag
     2881 gataactgat gggcctgtcc atttaattgt acttgtcgca cccatgaaaa gccccacgcc
     2941 aatcgtacca ccaagtgcaa tcatttgaat ttgacgggca ctcaatcccc tttgtagttc
     3001 attgttctcc ataacttctt accccatctc tgattgatac taaataaatc cataaaaaaa
     3061 cttattgccg taattatact ataaattatc gattttggta cattaatatt tgcataccat
     3121 gcaatcgttt acagaactac tatagcaggc ttttgcaact ttgcaaacaa ttattttgtg
     3181 atttttctaa aaaattaaaa aagttgctca agtaccgttg agtcacatac aacttcagct
     3241 attcttggta attatgatat aattttaata atattaaaaa ggagtatatc aatgttaaaa
     3301 caaatactgc tacgtgctac aaaaatatcg gtactctttg ctgtagcatt tttcatcata
     3361 aattatatcg gtatggaaaa acctgacatc ctatatttag ttggaagaac tattattgca
     3421 acacttgctt tcatactcat ttgtcttaca gtatttacta ttattaattc tccagagcgc
     3481 aagattaaac tcggtacgac tttaccgatt gcattaatta ttggtatcat tttcggcgcc
     3541 atatttttaa cggtacaaat cggtgtcatc actgggttaa tcatcggtgt gattgctacg
     3601 tttatttggg aattgattga aaaaaataaa ggaggtcgtt catcatgaca acagcattaa
     3661 ttggtgtaat tgtattaatc ttactaatta ttagctttgt gccaaactac cgtgctatga
     3721 aatcagcaaa agaacaagga agtaatgcaa caagagctac aattatggta ggcatagata
     3781 ttatcttaat tgtacttatc atcgtgacgt tattattaaa atatatgtaa ctatttaaac
     3841 caccctatct catacataaa catgaacgtt ctaccatttc acatacatat ggtcgtccat
     3901 ttaatgaggt aaggtggttt tctcgttaaa gtttaccttg taatagtaaa aaatagccaa
     3961 ttgttgcgag tattaagtaa atcattgaac caacaacaaa agatcttact tgcacgcgaa
     4021 atggtgtctg tttgttaaat ataggaccaa tcaccaaatt tccaacgcct aaaattactg
     4081 gaccagctag aaaaaacaat ataatgtaac ccatcatgat acgcctccta tttcgtaaag
     4141 aaacgcgcta caatttgatc gtaatcaata tcttttgcta cataatctac attagaaaag
     4201 tcctgtgcgc ttgtcatttt attcgtatta acaataacat ctaatccagc tgcaatcgcg
     4261 gctgttgcac catttacaga atcttcaata gccaaacatt gtgtcggcat ataatttaat
     4321 tgttgtactg cagataaata taattcaggg tcaggtttaa cttgttcaac atcttctcta
     4381 ccaacaacta cctctatata atcgtctaga cctaatgctt taaaggtggg cattatttcc
     4441 gctttcacac tacttgtggc aattgccata ggaatgtgac gttgctttaa aaatgccatc
     4501 aatgatttaa tcgtatcaat catcggtaat gttgtactag taagatgatg ttcttcataa
     4561 agtttatctt tattttccga accaatcgct ttaattaaat ggtcatgcaa atctgtagct
     4621 gctcctccaa tagaagaacg ataaaaatca atgcttatag gatcggcatt atgcatctct
     4681 aaatgtttat taataacatt aaataaatgt tgttccgtat ctattattgt tccatcgaaa
     4741 tcaaatataa ctgctctata catatattta ctcctctatt ttttctcttt atcaaagctt
     4801 aacatagtcc gatgaccact tcattctatt agtgcaatta taactttttt gaccaatact
     4861 ttgctaaaat aggccctgtt atattatgaa tgaaactaaa cactgcgcct ggtactgctg
     4921 caattggatt aaaatgcaat gctgctaatg acacagctaa accagagttc tgcattccaa
     4981 cttcaatact gactgctttt tgatcttgtc gatctaattt taaaagacga gctaaccaat
     5041 atccaatcgt ataccctaat acgttatgta aaataactac taaaaatatt aataatcctg
     5101 tagttaagat ttggtgctta ctgccaccta caactattgc taaaattaat gaaatagcaa
     5161 caactgatat aattggcaaa gctgtagaag ctttttcagc aattttacga ttaataattt
     5221 gcaaaacaat acctaaagca attggaatta atacaacttg aacaactgac cacaacatac
     5281 tcacgaaaga tacttccaac cattcatttg caaatagata tattaacgca ggtgtaacga
     5341 atggcgctag caacgtagag accgtcgtaa tagaaacaga aagtgctacg ttagctttgg
     5401 ctaaataact cattacatta cttgatgtcc cacccggaca acatccgact aatattacgc
     5461 caacagcaat atcaggtggt aaatgaaaag actttgcaat tataaatgct aatgtgggca
     5521 taatactgaa ttgtagacag acaccaataa ttactgctct aggtgcttta aagaccattt
     5581 tgaaatcatt aggtgtaatt gttaatccca tacctaacat aacaatacca agtaaataag
     5641 gtacccattt acctaatgtc gcaacatgtt gtgggaaaat aaatccaata acagctgcta
     5701 ctaacatcca taccaaaaaa ctcttcgtgg caaatatact caatttttga aacattgtta
     5761 acacatcctc gtcgataata ttgaaaaata ttaccataag accagtaagg ttacaattac
     5821 ttttctgaaa attaagatta tttagtcatt ctcttcttat tgcaaatatt ttatcactga
     5881 taaacaatta ttttaaactt cattcaaaag tcttagtgaa taaagaaaaa aagcgtggta
     5941 aaatcaactt gaacaaaatt aagtcacctt tataaatatt aaacgtgtat ttaatgccat
     6001 gtattcctcg ggaggtcctc acatgttaat tgataaattc gaaacataca tcatcaacat
     6061 cgccggttta aatgaccgta ctactagaaa aaaattaagt aagttatgca aaagtgtaca
     6121 attttgtgat gcgttgcaat tctcaataaa caaacagttt aatcagtatg tattagaaat
     6181 tagcttacca aagcaacaac ttccatactt catttcattt ttaagttttc atcagtatag
     6241 tattttccaa gtactttcac caaagaaaat aaatgaatta cttgattctg ataatttata
     6301 tcaatctgca aagcgtttcg atatcaatat tgatggcttg caagatgctt tcattaaaga
     6361 caaagtcatc gatattatga atatgttcca aaatcacact gatatcactt atacgttgaa
     6421 taagtctcat gcacatatta tatgtacgcc agaaatattt gctaaattgt tacatacgat
     6481 tgcaactaga aatatcgaca ttctatctgc caattataga tcgtcatcta tgagcaaagc
     6541 tcgtatatca taataaaaga cacatctata cgatgatcat ttacaatgat tgcgtatagt
     6601 gatgtgtctt tttttgtatt atttatcttc ggacgattga tcctcaacca tttgttcaaa
     6661 tgcttcacga acttgtggta ctgtcatacc tactacaact tggatactct taccactctt
     6721 tactaatccg tgtgccattt gttgatgcgt gaaatattcc gtatctgcaa ctttactttc
     6781 atcataaaca gttaagcgta atcttgttgt acagttagta acatctttga tattttcttt
     6841 gccgcctaaa ccgtcgaggt aatatgcagc tttgtcttcg tattcattac cagatgaagc
     6901 acgtttagaa tctacgctat cacctttttt atttttgtaa tcttgcttag aaaataattt
     6961 aacttcctct tctgttttct tacgtccagg taatggaata tcaaatttca aaattaagaa
     7021 tctgaataag aagaagtaaa tcgctacaaa tatcagaccg ataataactt gtgcaacata
     7081 agtaccccaa tgttctttac ctaatggcaa ccagtttgtt gaaataaagt ctaacaagcc
     7141 accacccatg ttacctacaa caccaaatgc gtacattaaa gtatccattg atgctgctaa
     7201 aactgcgtgt aaaacaaata agtatggtgc gatgaataag aatgtaaact ctaatggctc
     7261 agtaatacct acaacgactg ctgttaatgt ggcaggaata agtagtgccg ccaccttttt
     7321 acgattttct ttaggtgtcg tagcatacat tgctaaagca atacctaaac atccaaatac
     7381 tttcccattt ccttgaagca taaagccata agggaattgc tcttttaatg gcttagtact
     7441 cttagcaaac tcatttaaat gttgtaacca ttcagctttc aagccgtgat tgactactac
     7501 tggtccaact tcaattggtg cataaataaa gtgatgtaat cctgttggta ttaatacacg
     7561 ttctaagaag tgatacaacc atacaccgat atagcctgaa gcaatgataa agtgttgcat
     7621 tgaaccaata ccgtgctgaa cagttggcca aataacacaa gtaattgctg ctaatggtaa
     7681 catcacaaaa aatgaaatcg taactacaaa tgttaaacct tggaatacac caaccatttc
     7741 tggtaatcgt ttactatagt atctattgtg aatccaagta ataatagctg aaataataat
     7801 accacctaga atattcgtat ccaatgtggc aatacctgca attgatttta aaccaggtac
     7861 attttcaacg cctttttcta aattagcgcc aaacgtatgt ggccattgtg ttaaaatggc
     7921 atttataaat gtattaaaca ttaagtaacc catcaatgct gcaagtgctg catgacctgg
     7981 tgctttttta gctaaagaaa gtggtagacc tacaacaaat acaacttcca tatgcgtaaa
     8041 aataacccag ccaccagatt ctattactga ccaaaattta aaccaaaacg tatgttgatc
     8101 tgctaaactc cccatgattg taggattttt aaataatgtc gcaaagccaa gcactatccc
     8161 aaagaaagcg aacattaata ccggtacaat cattgcacta ccgaaacgct ttatcgcatt
     8221 catcttctat tccctccata tcatctttcc taacaataca tctaattaga ttcatttata
     8281 aatagatgtc ttactattta aatataatat atagtaaacg cttacacacc tacaacgaca
     8341 ttgacgtatt ttgaaagtat tttgtataat cagattatct tttcatatag tgaaaatttt
     8401 ttcacgacct tatatatgac atcgttgtat ttgtaataca ttcgttttaa acgcataatc
     8461 aaacctatat caatacacaa atatatataa tgacatacaa gattttaatg taataacgat
     8521 ctattacaca tttattttca aggaggttga atatgttttt agatgaacac attaatcgaa
     8581 actttgataa acttaatgat aatgatttac atatcgctca ctttatcaat acacatatag
     8641 atgaatgtaa aaatatgaaa atacaagatt tagcgcaatt cacacatgcc tcgaatgcaa
     8701 ccattcacag atttacacgc aaattaggtt ttgacggtta tagtgatttt aaatcgtacc
     8761 ttaaatttga aagtaataag acacaccaat taccttctaa ctctatcgat agtttcaaac
     8821 aagaaatcga tagtacattc agttatttag aacgcgttga ttaccagctt cttactaaaa
     8881 aaatgcatac agcttcaaca atatatttgt atggtacagg gcgtgcacag ttaaacgttg
     8941 cttcagaggc acagcgtata ttgttaacgt tacataatca tgtcattgta cttcatgatg
     9001 aacatgaact taaaatgatt ttgaacaaga gtgaacctaa tgatttattc tttattattt
     9061 cattatcagg tgaaacacat gaattattag aagtaacaaa cttgcttcaa cttagacaaa
     9121 aatactttat ttctgttact acaatgaaag ataataccct tgctcaaaaa gcaaattaca
     9181 acgtctatgt atccagtaac acattctact taactgatgg tacagactat tctagcttca
     9241 tcagttatca tatattcttt gaaacacttg taagaaaata taatgagtat ttactacata
     9301 atgatatttc aaattagaaa aaaggaagtt atgatttaat ggccttgagc ctatcataac
     9361 ttccttttat cattttattg ttgtgttgat gtttcgataa cgtggtacat cttatcaaac
     9421 atcaattcga aaccatgcac catggcatca tgatattctt ttttcttttg cttgtattct
     9481 aaattagtaa atcgtctttc tttttcaact aatgaacgat aataaaatag catttgggtg
     9541 ccacctgttt cacgttcaaa aaattctacc tcaatgacat cttgcgtttc acttagtcca
     9601 ggcataccga tagtcatctt aacgtattca tccataacta aagattcata aatgccttca
     9661 atcacattta ctttgccatt acgttgttga tctacaatac gatatttacc gccttcttta
     9721 acgtccgctt caatctcttt attcgttctg gctgatgtca taaaccattg tttcaacaaa
     9781 tctttctttg tccaagcttc gtatactaac tctggagaaa atttataaag cttttcaatt
     9841 tcaacttcga catgttcatt ctctacatta aattttgcca ctgttgtcca cccactttcg
     9901 ctcttacttt tattttaacg tatttttgct cagttccaaa catagatgat catcattttt
     9961 aaaagattag cgttatacgg tgagtacaac atgatctgtt aatataacaa gccaccttac
    10021 ttggctacat cgatatattg ttaagcatta atgtttcatt tcttgactag tgttcttttt
    10081 tagctttgga aaattaaata aaatcgcaat aagtccgcat acacctaata atataggata
    10141 aatgctgtat gggaataaca ttaacggtga aataccagct acaccagccg ctgaaatgac
    10201 ttgcgggcta tatggtaata aaccttggaa gcagcctcca aatatatcaa gaatacttgc
    10261 tgatttcctt gaatctacat catattcatc tgcaatattt ttagctaaag gacctgacat
    10321 aataatagag atggtgttgt ttgccgtggc aatatctgcg acacttacca aactagcaat
    10381 tcctaattct gcgccacgct ttgatttcac tttagagcga acaaattgca acaaccattc
    10441 aataccacca ttgtgttgaa taataccgac taaaccacca attagcaacg caatcatagc
    10501 aatatcttcc atgcttataa tacctttgga cactgcatct agtagcccca tccaaccgaa
    10561 tgaaccatct atgagaccaa tgattccggc taataatgtt ccgccaatca atacgataat
    10621 gacatttaca cctaataatg ctaataccaa tactaagata tacggtacaa ctttaattag
    10681 attataatca tagtttttag catgatttaa agaaatgcca ttcgttaaga aatacagaat
    10741 aataatcgtt aaaatagcac ctggcaatac aattttaaag tttactctga atttatcttt
    10801 cattttcgta tgttgtgttc taaccgcagc aattgttgta tctgaaatca ttgatagatt
    10861 atcgccgaac attgcacctc caacaactgt agccattgct agcgcagttg gtacatctgt
    10921 cgcttgtgca aatccgtaac caacaggtgc tattgcagca acagttccta cagacgtccc
    10981 catagatata gaaacaaaca tacaaatcac aaacaatcct acaataatta aattttctgg
    11041 gattaatgat agtcctaaat taactgtcga ctttacgcca cccatttttt cagctgtatt
    11101 tgaaaatgca cctgctaaaa taaaaatcaa catcattaaa acaatgtttg aatggcctgc
    11161 acctttcgtg aagacctcaa cttttttagc aaatgattct tttcgattca ttaataacgc
    11221 cacaattacc gttatcgtaa ttgcaacatt taatggcatt gaagtaaaat cacctgtgat
    11281 aatacctacg cctaaaaaca acgccacaaa taataacaag gggaataatg cccaagcatt
    11341 gctcttttta tgtacttcca tcctttttac ctgctttcca attaaaaata cctctttctc
    11401 acaaacgatg aagaaagagg ttttcatgtg ctttacctgc ttatcttcaa accattacgg
    11461 ttactggaat tggcacattc gagatgttgc cgaggcttca tagggccagt ccctccacct
    11521 ctctagataa gtgatgctta tttacgttta cgttacaaga taatccttag tacgtcaatc
    11581 ataaattaat caggagtcgt ataatatttt tcataaacaa tcattgctac tgtaataata
    11641 atcaaaacaa taatgctaat aacaagtaaa agccaccatt taagcattaa tgcaataaaa
    11701 atgaacacga tagacacact tactaatatt aatgatatga ctttaaattg ctgaacacgt
    11761 tgcttggaga tgactttcaa ctgtttgttt gatagacgcg tattttttat actgattccc
    11821 agtatatttt ctaatatttg aaccaatacg atacttattg caaatataat aattggtaaa
    11881 acatcatagc tccctatagt taatgtataa attacaaatc caatgtaaag taaccctgag
    11941 acaaaggata aaaagtatgc gacgtatttg ttaaacttaa tgatatgctt tttaacgttt
    12001 tgatgtgtaa accatacatt cgaaacgatc gcaactgcta caaataatgt gaatactata
    12061 tataatggta atttttgttc aggaaaaaca gtcgctattc caaaagctaa tgctaaaatc
    12121 aaaaataata tagctctaga tactattaat gccataataa caaccccttt gtttaatatc
    12181 gagtttgcaa atttacgttt atcagcgttt ctatgatcag tacttctacg ggtagcgttt
    12241 ctatgtaatt tacatcatct taacatataa atacttcgct atttaattga aaacatatcc
    12301 tattattctt tgtccgttct gacgtttaat atctagcctt aggcatttca cttgttaatg
    12361 aatttaactt tcttccacta accgtcccta aacccaatcc cgcaacagtt tttaactttt
    12421 tcgttgttgt cctgacatcc tcattaagaa agtttattct gcttaaaact tataatccac
    12481 accctgagca aacgctcctt atgacagagt attaaaataa gccgataaag atacacacct
    12541 ttaccgacta tttaaaatac acttcaccaa ttcattttaa tttaatggat tgaagtaact
    12601 aaattaatat tatgttgttc aattaaaagc ttcatacaaa cctaatctat ttgcactcca
    12661 ccgctaacac cgaacacttg tccggttgta taacttgatt cttctgacgc taatagcaca
    12721 taagttccac ataactcaac aggttgacct gcacgaccta aaggtgtttt ttgaccaaat
    12781 gttgggattt tactttgagg ttgtccacca gaaatttgta atggtgacca gaatggacca
    12841 ggcgctacac agttcactct aattcctttt ggtcctaatt cttctgaaaa acttttagtt
    12901 aatgaaataa ttgctgcttt tgaagcggca taatcatgaa gaataggact aggattataa
    12961 ccttgtacag atgatgtcgt tgtaattgac gcacccggtt ttaaatattc caatgctttt
    13021 tgaactgtcc aaaatagcgg atagacattc gtttcaaatg tttctgtaaa tgcctcagtt
    13081 gtaaatccat gaatatcatc atgatactgt tgatgtccag caactaaagt aacattatct
    13141 aagccaccta attgttgata tgcttgttca acaaggtcat agttgaactg ttcatctctt
    13201 atatcaccag gaattaacac tgccttttga ccactttctt caatcacttg gcgtacttct
    13261 tgtgcatctt gttcttcact cggaagatag ttaatcgcta catctgcacc ttctttagca
    13321 tacgcaattg ctgctgcacg ccctattgct gagtcaccac ctgtgactaa tattttatag
    13381 ccttgtaagc gttgatgacc ttggtaagac gtttcgccac aatcgggtgc tggcgtcatt
    13441 tcagattgta aacccggtac ctcttgttct tgtttttcat aatccgttgt tttaaatttt
    13501 gttctaggat cttgagctgc cattttttta catctcctta ttcgcttaat ggttattatt
    13561 tacccaatct tcctaggaac ttaatcatga ttacactaaa aattactttc ttctttataa
    13621 aaacaagctc gaattattca tgcaatagtc tctttacaaa ttcaacaaaa tactcaggta
    13681 ctttttccag aatcctttca tccggtttat attgaggatg atgtaaatca tattcactat
    13741 gagaaccaat taacgcaaat acacttggaa aatgttgact ataacctgaa aaatcttctc
    13801 caatcgtaag cggctgttcc atcatttcca ccttatatcc aacatgttgg gctactgcaa
    13861 ttgctttatg cgtcaatgcc tcatcattca tcacagcgcc aggtaaatgc gtataattta
    13921 aattgatttt catattatat gcttgagcca atccgtccgc aatatcttgt aatcgtgttt
    13981 ctacaagctt tcgtaccaca ggatcaaaac tacgcactgt gccttgtaca tacgcatgat
    14041 cagcaatgac attccaagta ttaccacatg atatttgtcc aattgttact accgcttcat
    14101 caaacgcaga tagatttcta ctaactatgg attgaatact attaatcaat tgcgccaaca
    14161 caataactgg atcgttgcat tgttctggct ttgcagcatg accacccacg cctttaatat
    14221 gaaactcaaa acgatctact gctgatgtaa ttgcccctgt tttgattgca aatgtaccta
    14281 ccgaacgcga tgggtcatta tgaaaaccca atactgcttg tacatctttt aatgcatgtg
    14341 tttcaataat tttaaaagcg ccatgtccta gttcttctgc tgattgaaaa atgaatttaa
    14401 cacgcccagt aagagtgccc tcaatttctt ttaattttac agctgtagcc aaaatactag
    14461 ccatgtgaat atcatgacca cacgcatgca taacaccttc attttcagat ttaaaatcct
    14521 gttcaacaag ctcttgaatc ggtaaagcat caatgtcagc ccgaacagct atacaactca
    14581 gaccttgtcc cacttcagca acaagcccag tcgcaagtgg taagtctaat attctaatat
    14641 gatgttctgt taaaatatct ttaatttttt gtgtagtctt aaattcttta tcggatagtt
    14701 ctggaaattg atgaaaatac cttctccagg taacagcttg atcttttaat cccatcggtc
    14761 attccccttc cttaagtcaa tgatatgttg tctaccctac gatgatcatc tttgactatt
    14821 aaacgatgat ttcacaacaa tgtactcttg ttaattgctt tcgttaatga tagacagttg
    14881 tttaataata tcgtaacact gttgtcaaac tattctaact tttataattg agactctata
    14941 caaaaacgtg ttctcgaata tacttgtttt tacaaaccac aaaaagctct aaacattagt
    15001 ttaaaccaat gcttagagct ttctaattat tttatgcttt aaaagatact gtgttatcta
    15061 cgatgacctt accgtcttta ataacttttt ctgcgtgatt gataccaaaa tgatatggaa
    15121 tatattcatg atttggtgca tcccaaatta ctaaattagc cttatcacct gtgttaattg
    15181 tacccgcgtt aatgtctatt gctttagcag cattgaccgt aacagcattc caaacttcat
    15241 taggtgatag ctttaatttc aaggctgcaa tcgccataac aagttgtaag ttgtttgtga
    15301 cactactacc agggttataa tcagttgcta atgcaatcgc accgttattg tcaagcatgc
    15361 ctcttgcatc tgcataatct tctttaccta aatagaacgt cgttgcaggt aagaggacag
    15421 ctacagtatc actatttcgc aacttttctt ttcctttatc actagaagct actaagtggt
    15481 ctgctgatat tgcttgttca tcaattgcta attccagtcc gcctaacgga tcaatttcat
    15541 ccgcatgtat tttcacttta aaacctgctt ctttggcttt ttgcatataa tgttgcgatt
    15601 gttctattgt aaatacacct gtttcacaga aaatatccgc aaagtctgca tattgtttta
    15661 cttccggaag taacgcaatc atttcttcta aaaatgcctc atttgaactt gcctctttag
    15721 gtacagcatg aggccctagg aaagtatgtt tcatgtctaa atcatatttc tcagctaaac
    15781 gattagacac tttcaattgc ttcagttcat tttctctatc taatccataa ccactcttac
    15841 tttcaactgc aagcacgccg tgtttaatca tagtaagcaa atcatgctct gcttttttaa
    15901 acaagtcatc ttcggatgtt tctctagtag cattaacggt agataatatg ccaccaccca
    15961 tttctaatat ttcaaggtaa gacttacctt gacgttttaa tgacatctca tgttctcgag
    16021 atccaccaaa tgttaaatgg gtatgtgcat ctactaatgc tggggacact accttcccac
    16081 tagcatcaat cgtctcagtc gcatcgtagt catctgtatg tgttccagca tatacaattt
    16141 tgccatcttt aatgacaact gtaccatttt tcacaacatt taattcatct aattccttac
    16201 ccttcaaagg tttatctgtt gatctcggta aaattaattc tgctatatga ttaattatta
    16261 aatcattcat tacttatcac ctgctttatc aatcattgga atatgaatac ctttttcttt
    16321 agctgtttga atagcgatgt catagccagc atcaacatgt cgggcaacac ccatacctgg
    16381 gtcagtcgtc aatacacgtt ccaatcttct ttcagcacgc tctgatccat ctgctacaac
    16441 aaccataccc gcatgaagtg aatatcccat gccaacaccg ccaccgtgat ggaatgaaat
    16501 ccatgaacca cctgcagctg tgttaatgag tgcattcaat acagcccaat caccaaccgc
    16561 gtcactacca tctttcatac tttctgtttc acggttagga ctagcaactg aaccagcatc
    16621 taaatggtct cgtccaataa caattggtgc tgaaatttca ccgtcacgta caagacgatt
    16681 taaagctaag cccattttcg ctctttctcc atagcctaac caagcaatac gtgatggtag
    16741 tccttgatat gaaatttttt cttcagctaa atcaagccat cttaataact tttcattttc
    16801 tgggaaaagt ttgcgcattt cttcatccgc acgctcgata tcttttggat caccactcaa
    16861 cgcagcaaag cggaatggcc ctttaccttc acagaataat ggtctaatgt aagctggtac
    16921 aaagcctggg aagtcaaaag catttttcac tccgttattg aaggctactt gacgaatatt
    16981 gttaccataa tcaaatgcta cagcgccacg tttttggaat tcaagcatta attcaacatg
    17041 ctttgccatt gaagcttgtg acagttcaac atattttttc ggatcttttt cacgcaatac
    17101 tttcgcttct tctacagagt atccttgtgg cacatatcca tttagcggat catgtgcact
    17161 tgtttggtca gtaataatgt caattttaaa tcctttttct agaatcgctt gatggatgtc
    17221 tacagcattt ccaactaacc cgattgataa tccttctcca cgttctttcg cctcttctgc
    17281 taattttaat gcttcatcta aatcagctgt tttaacatca cagtatttcg tatcaattcg
    17341 cttatcaaca cgtgtttcat caacatccac gcaaattgct accccatgat tcatagtaat
    17401 tgctaacggt tgcgcaccac ccataccacc taaacctgct gtcagtgtaa cagtgcctgc
    17461 taaatctcca ttaaagtgtt gattacctag ctcggcaaat gtctcataag taccttgcac
    17521 aataccttga gaaccaatat atatccaact accggctgtc atctgtccat acatgattaa
    17581 acctttttta tctaattcat taaaatgatc ccagtttgcc cattcaggca ctaatactga
    17641 atttgaaatt aatacacgtg gcgcttcttc atgtgtttta aatacagcaa ctggctttcc
    17701 tgattgtact aacattgtct catctgattc taattctcgt aacgttttct ctattgcttc
    17761 aaaagcttcc caattacgtg ctgcttttcc aataccacca taaacaacta aatcttctgg
    17821 tctttcagca acttctgggt ctaaattgtt gtataacatt ctaagtactg cttcttgttc
    17881 ccaaccttta cactcaatac tcaaaccttt ttttgcttga atttttctca taaaattcgc
    17941 tcctgttctt ttaagaagtt aattccacta aatttaaaac gcttacatta ttatcttcaa
    18001 tattcattat agtatgttaa aatatagcca acaaatataa ataaactaat tatccatagc
    18061 ttgaatctat aaataaaagg agcaaaacac atgaaaatta ttcagttaga atacttcttg
    18121 gctatcgtga aatataatag ttttactaaa gctgcacaat ttttacatat tagccagcca
    18181 tctttaactg ctacgattaa aaaaatggaa gcagatttag gttatgactt atttacacgt
    18241 tcaacaaaag acatcaagat taccgaaaaa ggaatacagt tttatcgtta tgcgagcgaa
    18301 ttagttcaac aatatcgatc cacgatggaa aaaatgtatg atttaagcgt tacatcagaa
    18361 ccaaggataa aaattgggac tcttgaatct acgaatcaat ggattgcgaa tttaattcga
    18421 aagcaccatt ccgactaccc tgaacagcaa tatcgtttat atgaaataca tgataaacat
    18481 caatctatag agcaattact gaattttaat attcatttag ctataacaaa tgaaaaaata
    18541 acccacgaag atataagatc cattccttta tatgaggaat cttacatttt attagcaccc
    18601 aaggaaacat ttaaaaatca aaattgggta gatgttgaaa atttgccact catattacca
    18661 aacaaaaatt ctcaagtgcg caaacactta gatgactatt ttaatagaag aaatattcgt
    18721 ccaaatgtcg ttgtagaaac agatcgattc gaatcagcag ttggatttgt tcatctcggc
    18781 ttaggttacg ctatcattcc gagattttat taccaatcat ttcacacgtc taatttagaa
    18841 tataaaaaaa ttcgtccaaa cttaggccga aaaatttata tcaattacca taaaaaacgc
    18901 aaacactccg aacaagtaca tacattcgta caacaatgcc aagattattt atatggactt
    18961 ttagaggctc tttaacttaa gttattagag cctcttatgc agttgcgcag atcatcgtat
    19021 aaaaattaat gacgtcattt caaaaatcga tacaaaaata atttattata aaaattctaa
    19081 gaaagaagtg aagcagatgt taaaatctat taatcatata tgcttttcag tcagaaattt
    19141 aaacgattca atacattttt atagagatat tttacttggg aaattgctat tgactggtaa
    19201 aaaaactgct tattttgagc ttgcaggcct atggattgct ttaaatgaag aaaaagatat
    19261 accacgtaat gaaattcact tttcatatac acatatagct ttcactatag atgacagcga
    19321 atttaaatat tggcatcaga ggttaaaaga taataacgtg aatattttag aaggaagagt
    19381 tagagatatt agagatagac aatcaattta ctttaccgac cctgatggtc ataagctaga
    19441 attacatact ggcacacttg agaacagatt aaattattat aaagaggcta aaccacatat
    19501 gacattttac aaataaggtg tcattataaa aaggcctctt gaactcc
//
LOCUS       DALAGD010000060        18808 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.061, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000060 DALAGD010000000
VERSION     DALAGD010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18808
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.061"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            66..1421
                     /gene="brnQ"
                     /locus_tag="O2701_001707"
     CDS             66..1421
                     /gene="brnQ"
                     /locus_tag="O2701_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCZ4293595.1"
                     /translation="MKKKLTFKENMFIGSMLFGLFFGAGNLIFPIHLGQAAGSNVFIA
                     NLGFLITAIGLPFLGIIAIGISKTSGLFEIASRVNKTYAYIFTIALYLVIGPFFALPR
                     LATTSFEIAFSPFLSPKQITLYLFIFSFVFFVIAWFFARKPSRILEYIGKFLNPVFLV
                     LLAIILLFAFIHPLGGISDAPISKQYQSHALFNGFLDGYNTLDALASLAFGIIIVATI
                     KKLGIENPTDIAKETIKSGTISIIMMGIIYTLLAIMGTLSIGHFKLSENGGIALAQIT
                     QYYLGNYGIVLLSLIVMVACLKTAIGLITAFSETFEHLFPKMNYLAIATVVSFISFLF
                     ANVGLTKIIMYSVPVLMFLYPLAIALIVLTLFSSKFHHSKLIYQCTIFFTMIAALVDG
                     LKASPEFISSTSFSQTLINFSQKYLPLSDIGMGWVVLSLIGFIIGFIIYKIKHRKIPQ
                     A"
     gene            1674..2858
                     /gene="rocD"
                     /locus_tag="O2701_001708"
     CDS             1674..2858
                     /gene="rocD"
                     /locus_tag="O2701_001708"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HCZ4293596.1"
                     /translation="MNSIIELTDYYSSNNYAPLKLVISKGKGVKVWDTDGKQYIDCIS
                     GFSVANQGHCHPTIVKAMTEQASKLSIISRVLYSDNLGKWEEKICHLAKKDKVLPLNS
                     GTEAVEAAIKIARKWGSEVKGITDGQVEIIAMNNNFHGRTLGSLSLSNHDAYKAGFHP
                     LLQGTTTVDFGDIEQLTQAISPNTAAIILEPIQGEGGVNIPPKGYIQAVRQLCDKHQI
                     LLIADEIQVGLGRTGKWFAMEWEQVVPDIYILGKALGGGLYPVSAVLANNDVMRVLTP
                     GTHGSTFGGNPLAIAISTAALDVLKDEQLVERSERLGSFLLKALLQLKHPSIKEIRGR
                     GLFIGIELNTDAAPFVDQLIQRGILCKDTHRTIIRLSPPLVIDKEEIHQIVAAFQDVF
                     KN"
     gene            2897..3928
                     /locus_tag="O2701_001709"
     CDS             2897..3928
                     /locus_tag="O2701_001709"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCZ4293597.1"
                     /translation="MIKVGIVGGSGYGAIELIRLLQTHPHVTIAHIYSHSKVDEPLKL
                     TFPHLQHIMQHFEALTVDNNDCDVIFFATPAPVSKTCIPPLVEKGIHVIDLSGAFRIK
                     NREIYEAYYKETAAAQDDLNHAIYSISEWQSFDNNGTKLISNPGCFPTATLLALHPLI
                     SEKIVDLSSIIIDAKTGVSGAGRSLSQRVHFSEMNENLSAYAIGNHKHKPEIEQYLSI
                     IAGQDVSVIFTPHLVPMTRGILSTIYVKLSSEYTTESLHKLMTSYYANQPFVRIRDIG
                     TFPTTKEVLGSNYCDIGIYVDETTQTAILVSVIDNLVKGASGQAIQNLNILYDFEVTT
                     GLNQSPVYP"
     gene            3940..5181
                     /gene="argJ"
                     /locus_tag="O2701_001710"
     CDS             3940..5181
                     /gene="argJ"
                     /locus_tag="O2701_001710"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HCZ4293598.1"
                     /translation="MKHQETTSQQYNFSIIKHGDISTPQGFTAGGMHIGLRANKKDFG
                     WIYSSSLASAAAVYTLNQFKAAPLIVTEDTLQKSKGKLQALVVNSANANSCTGQQGID
                     DARQTQTWVAQQLQIPSEHVAVASTGVIGEYLPMDKIKTGTEHIKDANFATPGAFNEA
                     ILTTDTCTKHIAVSLKIDGKTVTIGGSTKGSGMIHPNMATMLAFITTDASIESNTLHQ
                     LLKSSTDHTFNMITVDGDTSTNDMVLVMANHQVEHQILSQDHPQWETFVDAFNFVCTF
                     LAKAIARDGEGATKLISVNVSGAKSISDARKIGKTIVSSNLVKSAIFGEDANFGRIIT
                     AIGYSGCEIDPNCTYVQLNQIPVVDKGMAVLFDEQAMSNTLTHENVTIDVQLGLGNAA
                     ATAYGCDLSYDYVRINASYRT"
     gene            5197..5961
                     /gene="argB"
                     /locus_tag="O2701_001711"
     CDS             5197..5961
                     /gene="argB"
                     /locus_tag="O2701_001711"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCZ4293599.1"
                     /translation="MKFIVIKIGGSTLSDMHPSIINNIKHLRSNNIYPIIVHGGGPFI
                     NEALSNQQIEPHFVNGLRVTDKATMTITKHTLIADVNTALVAQFNQHQCSAIGLCGLD
                     AQLFEITSFDQQYGYVGVPTALNKDALQYLCTKFVPIINSIGFNNHDGEFYNINADTL
                     AYFIASSLKAPIYVLSNIAGVLINDVVIPQLPLVDIHQYIEHGDIYGGMIPKVLDAKN
                     AIENGCPKVIIASGNKPNIIESIYNNDFVGTTILNS"
     gene            6231..6710
                     /locus_tag="O2701_001712"
     CDS             6231..6710
                     /locus_tag="O2701_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HCZ4293600.1"
                     /translation="MGIFIYAGIIGGLLSGIVKLGWEVMFPPRTPERNATNPPQELLQ
                     QLGFSSEFTHQTYTFSNMELPWVSFIVHFSFSIVIAIIYCILVKKYAYLAMGQGAVFG
                     IAIWVLFHLIIMPIMHTVPAVWDQPFQEHLSEFFGHIVWMMTIELVRQHFVYRYKLN"
     gene            complement(7038..7682)
                     /locus_tag="O2701_001713"
     CDS             complement(7038..7682)
                     /locus_tag="O2701_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCZ4293601.1"
                     /translation="MTVFVMQLQSNLKSIEELISQSRWSYKKPRTVNYRYNQDKLMHR
                     LGDILVQYGIQHDTGLLPHEWHYHISPRGKADIVQHNRDGQPIYVSLSYSYPYIVCVV
                     DKEPVGIDIEKISQRLDWRTLVTCFSTNEAHQICSLNDFYQIWTQKESFTKLIGEGLI
                     KGLDIYDMTQSHFYQSREVKFKQFIFDQFMVQVCFLGQAPWGYKKVSVFQLLSS"
     gene            complement(7695..14870)
                     /locus_tag="O2701_001714"
     CDS             complement(7695..14870)
                     /locus_tag="O2701_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HCZ4293602.1"
                     /translation="MIMGNLRFQQEYFRIYKNNTESTTHRNAYWVKLAKNVEATKMMY
                     ALSTIVQQHASIRHFFDVTTDDNLTMILHEFLPFIEIKQVPSSSANYDLEAFFKQELS
                     TYHFNDSPLFKVKLFQFADAAYILLDFHVSIFDDSQIDIFLDDLCNAYRGNTVINNTR
                     QHAHINRNDDKDNQDASHIALDSNYFRLENNSDIHIDSYFPIKHPFEQALYQTYLIDD
                     MTSIDMASLAVSVYLANHIMSQQHDVTLGIHVPSHLPNDLHGNIVPLTLTIDAKDVCQ
                     RFTTDFNKCVLQNMSQLQCAKSSLSLETIFHCYHHMMSCCNDVIEDVHQIHDAHTSLA
                     DIEIFPHQHGFKIIYNSAAYDLLSIETLSDLVRNIYLQITEENGNKRTTVDELNLMTE
                     RDIQLYDDINLSLPEIDDAQTVVTLFEQQVEATPNHVAVQFDGVFITYQTLNARANDL
                     AHRLRNQYGVEPNDRVAVIAEKSIEMIIAMIGVLKAGGAYVPIDPNYPSDRQEYILKD
                     VTPKVVITYQALYENGKQNINHIDLNKIAWKNIDNLSKCNTLEDHAYVIYTSGTTGNP
                     KGTLIPHRGIVRLVHQNHYVPLNEETTILLSGTIAFDAATFEIYGALLNGGKLIVAKK
                     EQLLNPIAVEQLINENDVNTMWLTSSLFNQIASERIEVLVPLKYLLIGGEVLNAKWVD
                     LLNQKPKHPQIINGYGPTENTTFTTTYNIPNKVPNRIPIGKPILGTHVYIMQGERRCG
                     VGIPGELCTSGFGLAAGYLNQPELTADKFIKDSNINQLMYRSGDIVRLLPDGNIDYLY
                     RKDKQVKIRGFRIELSEVEHALERIQGINKAVVIVQNHDQDQYIVAYYEAMHTLSHNK
                     IKSQLRMTLPEYMIPVNFMHIEQIPITINGKLDKKALPIMDYVDTDAYVAPSTDTEHL
                     LCQIFADILHVNQVGIHDNFFELGGHSLKATLVVNRIEASTGKRLQIGDLLQKPTVFE
                     LAQAIAKVQEQNYEVIPETIVKDDYVLSSAQKRMYLLWKSNHKDTVYNVPFLWRLSSE
                     LNVAQLRQAVQRLIARHEILRTQYIVVDDEVRQRIVADVAVDFEEVNTHFTDEQEIMR
                     QFVAPFNLEKPSQIRVRYIRSPLHAYLFIDTHHIINDGMSNIQLMNDLNALYQHKLLL
                     PLKLQYKDYSEWMSHRDMTKHRQYWLSQFKDEVPILSLPTDYVRPNIKTTNGAMMSFT
                     MNQQMRQLLQKYVEKHQITDFMFFMSVVMTLLSRYARKDDVVVGSVMSARMHKGTEQM
                     LGMFANTLVYRGQPSPDKMWTQFLQEVKEMSLEAYEHQEYPFECLVNDLDQSHDASRN
                     PLFDVMLVLQNNETNHAHFGHSKLTHIQPKSVTAKFDLSFIIEEDRDDYTINIEYNTD
                     LYHSETVRHMGNQCMIMIDYILKHQDTLQICDIPNGTEELLNWVNTHVNDRMLNVPGN
                     KSIISYFNEVVSRQGNHVALVMNDLTMTYETLRNYVDAIAHMLLSNGVGNGQRVALFT
                     ERSFEMIAAMLATVKVGASYIPIDIDFPNKRQGAILEDAKVTAVMSYGVEIETTLPVI
                     QLENAKGFVESKENEQYDDLHGNQLENTAMLDNEMYAIYTSGTTGMPKGVAIRQRNLL
                     NLVHAWSTELQLGDNEVFLQHANIVFDASVMEIYCCLLNGHTLVIPDREERVNPEQLQ
                     QLINKHRVTVASIPLQMCSVMEDFYIEKLITGGATSTASFVKYIEKHCGTYFNAYGPS
                     ESTVITSYWSHHCGDLIPETIPIGKPLSNIQVYIMSDGLLCGIGMPGELCIAGDSLAI
                     GYINRPELMADKWQNNPFGKGKLYHSGDLARYTSDGQIEFLGRIDKQVKVNGYRIELD
                     EIENAILAIRGISDCVVTVSHFDTHDILNAYYVGEQQVEQDLKQYLNDQLPKYMIPKT
                     ITHIDCMPLTTNDKVDTTRLPNPSPIQQSNKVYSEPSNEIEQTFVDVFGEVLKQNDVG
                     VDDDFFELGGNSLEAMLVVSHLKRFGHHISMQTLYQYKTVRQIVNYMYQNQQSLVALP
                     DNLSELQKIVMSRYNLGILEDSLSHRPLGNTLLTGATGFLGAYLIEVLQGYSHRIYCF
                     IRADNEEIAWYKLMTNLNDYFSEETVEIMLSNIEVIVGDFECMDDVVLPENMDTIIHA
                     GARTDHFGDDDEFEKVNVQGTVDVIRLAQQHHARLIYVSTISVGTYFDIDTEDVTFSE
                     ADVYKGQLLTSPYTRSKFYSELKVLEAVNNGLDGRIVRVGNLTNPYNGRWHMRNIKTN
                     RFSMVMNDLLQLDCIGVSMAEMPVDFSFVDTTARQIVALAQVNTPQIIYHVLSPNKMP
                     VKSLLECVKRKEIELVSDESFNEILQKQDMYETIGLTSVDREQQLAMIDTTLTLKIMN
                     HISEKWPTITNNWLYHWAQYIKTIFNK"
     gene            complement(15317..16567)
                     /locus_tag="O2701_001715"
     CDS             complement(15317..16567)
                     /locus_tag="O2701_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ4293603.1"
                     /translation="MKRLSTTLKVRLISNFLQLIITTAFIPFIALYLTDMLSQSIVGI
                     YLVGLVVLKFPLSIISGYLIEIFPKKLLVLIYQATMVIMLVFMGVFGSHQLWQIIGFC
                     VAYAIFTIVWGLQFPVMDTLIMDAITEDVEHYIYKISYWMTNLSVAIGALLGGLMYGY
                     SMLLLFLIAACIFLIVLFILYIWLPQDRNQVKQSDDKRHASRYQKLQIMNIFRSYKLV
                     LKDRNYMLLISGFSIIMMGEFSISSYIAIRLKDQFETISIGSYDITGAKMLAILLMIN
                     TVVVILLTYSISKVVLKIDFKKALITGLLIYIVGYSGLTYLNQFGLLVVFMIIATVGE
                     IIYSPIVSEQRFKIIPKAKRGTYSAVNALGIHFSETLARLGIVLGVFLTSLQMGLYMF
                     IVLTIGASMLVAGVFGGQKQVNTN"
     gene            complement(16953..17978)
                     /locus_tag="O2701_001716"
     CDS             complement(16953..17978)
                     /locus_tag="O2701_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HCZ4293604.1"
                     /translation="MKIVALFPEAVEGQENQLLNTKKALGLKTFLEERGHEFIILADN
                     GEDLDKHLPDMDVIISAPFYPAYMTRERIEKAPNLKLAITAGVGSDHVDLAAASEHNI
                     GVVEVTGSNTVSVAEHAVMDLLILLRNYEEGHRQSVEGEWNLSQVGNHAHELQHKTIG
                     IFGFGRIGQLVAERLAPFNVTLQHYDPINQQDHKLSKFVSFDELVSTSDAITIHAPLT
                     PETDNLFDKDVLSRMKKHSYLVNTARGKIVNRDALVEALASEHLQGYAGDVWYPQPAP
                     ADHPWRTMPRNAMTVHYSGMTLEAQKRIEDGVKDILERFFNHEPFQDKDIIVASGRIA
                     SKSYTAK"
     gene            complement(18151..18510)
                     /locus_tag="O2701_001717"
     CDS             complement(18151..18510)
                     /locus_tag="O2701_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HCZ4293605.1"
                     /translation="MNTIDTHTKEQQFSNLVRSYRKEYVGKGPNSIRVSFKDNWAIAH
                     MTGVLSKVESFYLNDKRNESMLHYTRTEKIKQMYKEIDVNEMESLVGAKFVKLFTDID
                     LNDDEVISIFVFDKSIE"
BASE COUNT         6090 a         3564 c         2882 g         6272 t
ORIGIN      
        1 aatttttgat attatcttaa ttaagtagat ttttataagt tctaaaaagg agaacaaata
       61 catatatgaa gaagaaacta acatttaaag aaaacatgtt tataggttct atgttatttg
      121 gtttattctt tggtgccggc aatcttatct tcccaataca cttgggtcaa gctgctggtt
      181 ctaacgtttt tatcgctaac ttaggatttt taattacagc aattggctta ccatttctag
      241 gtatcattgc tattggcatt tcaaagacat ctggtttatt tgaaattgca tcgcgtgtta
      301 ataaaacata tgcttacatt ttcacgattg ccttatatct agttatcgga ccatttttcg
      361 ccttacctag actggcaacg acatcatttg aaattgcatt ttcgccattt ttatcaccaa
      421 agcaaatcac tttatattta tttattttta gcttcgtctt ctttgtgatt gcatggtttt
      481 ttgcgagaaa gccatcaaga attttagaat atatcggtaa atttttaaat ccggtattct
      541 tagtattatt agcaattatt ttattatttg cttttatcca tccattaggt ggcatatctg
      601 atgcacctat tagtaaacaa tatcaatcac atgccttatt taacggcttt ttagatggat
      661 acaatacctt agatgcgcta gcgtcattgg catttggtat tatcattgtt gcaacgatta
      721 aaaagttagg tatcgaaaat ccaactgata tcgctaaaga aacaattaag tctggtacta
      781 tcagtatcat tatgatgggg atcatttata ccctactagc aatcatgggt acattaagta
      841 ttggtcattt caaacttagt gaaaatggtg gtattgcctt agcgcaaatt actcaatact
      901 acttaggtaa ctacggtatc gtcctgttgt cacttatcgt tatggttgct tgtttaaaaa
      961 cagccatcgg tttgattacg gcattttcag aaacattcga acaccttttc cctaaaatga
     1021 attacctagc gattgcaaca gttgtaagct ttatttcgtt cttattcgcg aatgttggtt
     1081 taactaagat tattatgtac tcagtcccag tgttaatgtt cttatatcca ttagcaattg
     1141 ccttgattgt actaacatta tttagtagca aattccatca ttcaaaactt atttatcaat
     1201 gtaccatttt ctttacaatg attgctgcat tagtagatgg attaaaagct agtccagagt
     1261 tcatttcaag cacatcattc tcacaaactt tgattaattt cagccaaaaa tatttaccat
     1321 tatcagacat tggtatgggc tgggttgttc tcagtttgat tggtttcatt atcggcttca
     1381 ttatttataa aattaagcat cgtaaaattc cacaagcata atactatgcc acagtcatat
     1441 gttaaacata tgcttgtggc attttttatt catactacat taaactgcaa tcgtatacat
     1501 acatatcaat gattatccac aaaaaatatt agtactttca ttttacaaat cacattaata
     1561 caaacacaac cttatcttta tattattaaa tttacatttg acacttatat tgaacaactg
     1621 taatatatta atattaattc tttaaaatgt ataaatataa aggagggaga ccgatgaatt
     1681 caatcattga attaactgat tattatagct ctaataatta tgcaccactt aagcttgtca
     1741 tttctaaagg taaaggtgtc aaagtttggg atactgatgg caaacaatat atagattgca
     1801 tttcgggttt ttcagttgca aaccaaggcc attgtcatcc aacaattgtt aaagcgatga
     1861 cagaacaagc ttcaaagttg tctatcattt cacgtgtcct ttatagtgac aatctcggga
     1921 aatgggaaga aaaaatttgt catcttgcta agaaagacaa agtactcccc cttaactctg
     1981 gtactgaagc tgttgaagca gccattaaaa ttgctagaaa atggggctct gaagttaaag
     2041 gcattactga cggacaagtt gaaatcatcg ctatgaataa caattttcac ggtcgtacac
     2101 ttggctcatt atcactatct aaccacgacg catataaagc aggatttcac cccctacttc
     2161 aaggcactac aacagtagat tttggagaca ttgaacaatt aacacaagct atttcaccga
     2221 atacagcagc aattattttg gaaccaattc aaggtgaagg tggcgttaat ataccaccga
     2281 aaggatatat tcaagctgtg cgtcaactat gtgataaaca tcaaatatta ttgattgcag
     2341 atgaaattca agttggtctt ggtagaactg ggaaatggtt tgctatggaa tgggagcaag
     2401 tcgttccaga catttatatt ttaggtaagg cattgggtgg cggcttatac cctgtatctg
     2461 ctgtacttgc aaataatgat gtcatgcgtg ttctaacacc aggtacacat ggttcaacat
     2521 ttggtggtaa ccctttagcc attgcaatat cgacggcagc gcttgatgta cttaaagatg
     2581 aacaactggt tgaacgatca gaacgcttag gttcattttt attaaaagcg ttgctacaac
     2641 ttaaacatcc tagtattaaa gaaattagag gtcgtggttt atttataggc atagagctta
     2701 acacagatgc tgcacctttt gtggatcaac tgattcaacg tggaatctta tgcaaagaca
     2761 cgcatcgtac tatcattcga ttgtctccac ctctagtcat tgataaagag gaaatccatc
     2821 aaattgttgc agcttttcaa gacgttttta aaaattaaca attaatcatt tatatatgac
     2881 ataggaggga ttcatgatga ttaaagtagg tatcgttggc ggtagcggtt atggcgcaat
     2941 tgaattaatt cgattgttac aaacacatcc tcatgtaacg attgcacaca tctactcaca
     3001 ttcaaaagta gatgaaccgt tgaaattaac atttccacat ttacaacata ttatgcaaca
     3061 tttcgaagca cttacagtgg acaataatga ctgtgatgta attttctttg cgacaccagc
     3121 acccgtaagt aaaacatgta tccctccctt agtagaaaaa ggtattcatg ttatcgattt
     3181 atctggcgca tttagaatta agaatcgtga aatatatgaa gcatattaca aagaaactgc
     3241 tgcagcacaa gatgatttga atcatgctat ttacagcatt tcagaatggc aatcgtttga
     3301 taacaatgga acgaagctca tttctaatcc tggctgtttc cctacagcaa cattattagc
     3361 attacatcca cttattagcg aaaaaatagt agatttgtca tctattatta ttgatgctaa
     3421 gaccggcgtg tcaggtgctg gtcgttcatt atcacaacgt gttcattttt cagaaatgaa
     3481 tgaaaatcta agcgcttatg caatcggaaa ccataaacac aaaccggaaa tcgagcaata
     3541 tttatctatc attgcgggtc aagatgtatc agtcatattt acaccacatc tcgtaccaat
     3601 gacacgaggt attttatcaa caatatatgt caaattatca tctgaatata cgactgaatc
     3661 attacataaa ttaatgacct cttattatgc taatcagcca tttgtcagaa ttagagatat
     3721 tgggactttt ccaaccacaa aagaagtact cggtagtaac tactgcgata tcggcatcta
     3781 tgtagatgaa acaacgcaaa cagcaatttt agtatcagtg attgataacc ttgtcaaagg
     3841 cgcaagtggg caagccattc aaaatttaaa tatattatat gattttgaag tgacgactgg
     3901 cctaaatcaa tcaccagttt atccataagg ggtgttagaa tgaaacatca agaaacgaca
     3961 tcacaacaat ataacttttc aattattaaa catggcgata tcagtacacc tcaaggcttc
     4021 acggctggtg gtatgcacat cggtttacgc gctaacaaaa aagactttgg atggatttac
     4081 tcatcgtctt tggcaagtgc agctgccgta tatactttaa atcagtttaa agctgcacca
     4141 cttattgtca ctgaagacac tttacaaaag tctaaaggaa aattacaagc acttgttgtt
     4201 aattcagcta atgcaaattc ttgtaccggt caacaaggca tagatgatgc acgacaaaca
     4261 caaacatggg ttgctcaaca acttcaaata ccatctgagc atgttgctgt tgcttcaact
     4321 ggggtcattg gtgaatattt gcctatggat aaaattaaga ctgggaccga acatattaag
     4381 gatgctaatt ttgcaacgcc aggtgcgttt aacgaggcaa ttttaacaac tgatacctgt
     4441 acaaaacata tcgctgtatc actaaaaatc gatggtaaaa ccgttacaat tggtggtagc
     4501 accaaaggtt caggtatgat tcacccaaat atggctacca tgcttgcttt tataacaacc
     4561 gatgcatcga ttgaatcgaa tacacttcat caattattaa aatcttcgac tgaccataca
     4621 tttaatatga ttactgttga tggcgataca agtacaaatg acatggtatt agtcatggca
     4681 aatcaccaag ttgaacacca aatacttagt caagaccatc cacaatggga aacatttgtt
     4741 gatgcattca attttgtctg tacattttta gctaaagcta tagccagaga tggcgaaggc
     4801 gcaacaaagt taatcagcgt caatgtgtca ggcgctaaat caatatcaga tgcaagaaaa
     4861 atcggtaaaa ctatcgtaag ttcaaatcta gtaaaatcag ctatttttgg cgaagatgcc
     4921 aattttggtc gaatcattac agctattggc tacagcggat gtgaaattga tcctaactgc
     4981 acatatgttc aactgaacca aatacctgtc gttgataaag gtatggctgt actatttgat
     5041 gagcaagcta tgtcgaatac attaactcat gaaaatgtca caattgacgt tcagcttggt
     5101 ttaggtaacg ctgcagcgac tgcatacggt tgtgatttat cctatgatta tgtgcgtatc
     5161 aacgcatcat atcgaacata aggtggtgtt ggttagatga aatttattgt cattaaaatt
     5221 ggtggcagta cacttagtga catgcatcca tcaattatta acaacattaa gcatttacga
     5281 tcaaacaaca tctaccccat tatcgttcat ggcggtggcc catttattaa tgaagcatta
     5341 tcaaaccagc aaatcgagcc acactttgtt aatggcctaa gagtgactga taaagcaacc
     5401 atgaccatta ctaaacacac gctcattgca gacgttaaca ctgcattagt agctcaattt
     5461 aaccagcacc aatgttctgc aataggctta tgtggtttgg atgcacagct gtttgaaatt
     5521 acatcttttg atcaacaata tggatatgtc ggtgttccga ccgctttaaa taaggatgct
     5581 ttacagtatt tatgtactaa atttgtacct atcatcaatt cgattggttt caataaccat
     5641 gatggagaat tttacaatat taatgctgac acgcttgcct attttattgc atcatcatta
     5701 aaagcgccta tttatgtatt aagtaatatt gcaggtgtac tcatcaatga tgttgttata
     5761 cctcaattgc cattagtcga tattcatcaa tatattgaac atggtgatat ttatggaggt
     5821 atgattccca aagtgctaga tgccaaaaat gcgattgaaa atggctgtcc taaagttatc
     5881 attgcatcag gaaacaagcc aaatatcatt gaatctattt acaataatga ttttgttggc
     5941 acaacaatcc ttaattcata actatgaaat taaggcctaa caagttttga cacgcgagat
     6001 gattccagtt cgattatcca ttgcgctaaa acatttattt accgttcatc tcgttaacaa
     6061 ttttgaatac agtacgatac aatatgagat gtaaaaaact aataaccttt tacaaatttg
     6121 tttatcaaaa tattttaagt tttgcaaagc tttttattgt gattattttc acaaaatact
     6181 ataatgagga tagtaaatag agaggagtcc ttaagttgac gaaacgacaa atgggtatat
     6241 tcatttatgc tggaattatc ggtggcttgt tatctggaat tgtaaaatta ggttgggagg
     6301 tcatgtttcc acctcgcaca ccagaacgta atgcaacgaa cccacctcaa gagttattgc
     6361 aacaattagg atttagtagt gagtttacgc atcaaacata tacattttca aatatggaat
     6421 tgccttgggt aagctttatt gtccacttta gtttttctat cgtcattgca attatttact
     6481 gcatattagt taaaaaatac gcttacttag caatgggaca aggtgctgtt tttggtattg
     6541 ctatttgggt attattccac cttatcatta tgccaatcat gcatactgta cctgctgtgt
     6601 gggatcaacc attccaagag catctgtcag aattctttgg ccatatcgtc tggatgatga
     6661 caattgaatt agtgcgacaa cattttgtct atcgctataa attaaattaa tacactgact
     6721 aacattaacg tgagttttaa atcatcgttt gagtatgatg attgatgctc acgttatttt
     6781 attaactgac atgatatgat tccagccaac ttacgtgagc attaaagtct caaatgcgtc
     6841 gtaacaaact attattttcg gtaatttcaa tattgctcag tatattttta ccttatcact
     6901 tactttaatc tcgtcatgat tttgaatgat gccatcgtgt attcaccttt catttttcca
     6961 ataaaaaaac atctaacagt aaacatttag gcagtatagt ttaaaatcac tgcgcaatga
     7021 tactgtcaga cgtcatatta actactcaat aactgaaata cagacacttt tttataaccc
     7081 cagggtgcct gtcctaagaa acatacctgt accataaact gatcaaaaat aaattgtttg
     7141 aacttcactt cacgtgattg ataaaagtgt gattgtgtca tatcataaat gtccaatcct
     7201 ttgattaaac cttcaccaat caattttgta aaactttctt tttgtgtcca tatttgataa
     7261 aaatcattta aactacatat ttgatgtgct tcgtttgtag agaaacacgt cactaacgta
     7321 cgccagtcta aacgttgtga tatcttttcg atatcaatac caactggttc tttatcgaca
     7381 acacacacga tataaggata actatatgat aagctcacat agatgggctg tccatcacga
     7441 ttgtgttgaa caatatctgc cttacctcgt ggcgaaatgt gataatgcca ttcatgtggt
     7501 aataaacctg tgtcatgttg aattccatat tgcactaaaa tatctcccaa tctgtgcatg
     7561 agtttatctt gattgtatct atagttgact gtacgcggtt ttttatatga ccaacgactt
     7621 tgtgatatta attcttcaat acttttcaag ttactctgta attgcattac aaatactgtc
     7681 ataactttcc ctacttactt attgaatatt gttttgatat attgtgccca atgatacagc
     7741 caattgttag ttatcgttgg ccatttttca ctgatgtgat tcattatttt taatgttaat
     7801 gttgtatcta tcattgctag ttgttgttca cggtcaacac tagttaatcc aatcgtttcg
     7861 tacatgtctt gtttctgtaa aatttcatta aatgattcat cgctgacgag ttcaatttct
     7921 ttgcgcttaa cgcattctaa caaagatttc accggcattt tattaggtga tagcacatgg
     7981 taaatgattt gtggtgtgtt gacctgtgct aatgcgacaa tttgtcttgc agtcgtatcc
     8041 acaaaagaaa aatctacagg catttcagcc atgctaaccc cgatacaatc cagttgtaac
     8101 aaatcattca ttaccattga aaaacggtta gtctttatat ttctcatatg ccatcttcca
     8161 ttgtaaggat tcgtcaaatt accaacacgt acaatccgac catctaagcc attatttaca
     8221 gcttctaata cttttaattc actataaaat ttgctccgtg tatatggtga tgttagtagt
     8281 tgccctttat agacatccgc ttctgaaaat gtcacatctt ctgtgtctat atcaaaataa
     8341 gttcccacac ttatcgtaga cacatatatt aaccttgcat gatgttgttg tgccaaacgt
     8401 atgacatcaa cagtaccttg aacatttact ttttcaaatt catcatcatc accaaagtga
     8461 tctgtacgag cacctgcatg aataatcgta tccatgtttt ctggtaaaac aacatcatcc
     8521 atacactcga aatcaccaac aatgacttca atgtttgata acattatttc aaccgtctct
     8581 tctgaaaaat aatcatttaa attcgtcatc aacttatacc atgctatttc ctcattatca
     8641 gcacgtatga aacaataaat gcgatgactg tatccttgta gtacttcaat cagataagca
     8701 cctaaaaaac ctgtcgcgcc agtcaatagt gtatttccta gaggtcgatg acttagacta
     8761 tcctctaaaa tacccaagtt ataacgagac ataacaatct tttgtaattc cgaaagatta
     8821 tccggtaatg caactaatga ttgttgattt tggtacatat aattaacaat ctgtcgcacg
     8881 gttttatatt ggtataatgt ctgcattgaa atatgatggc caaatcgttt taaatgcgag
     8941 acaactaaca tcgcctctaa tgagttacca ccaagttcaa agaaatcatc gtcaacaccg
     9001 acatcatttt gtttcaatac ctctccaaat acatcaacaa atgtctgctc aatttcatta
     9061 gagggttcgc tatacacttt attagactgt tgtataggtg atggatttgg caaacgcgta
     9121 gtatccacct tgtcattcgt ggttaatggc atacaatcga tatgcgttat agtcttagga
     9181 atcatatact taggcagctg atcatttaaa tattgcttca aatcctgttc cacttgttgc
     9241 tctccgacat aataagcatt caatatatca tgcgtatcaa agtgacttac tgttacaaca
     9301 caatcagata taccacgaat agctaatatt gcattttcaa tttcatcaag ttcaatacgg
     9361 tacccgttaa ctttcacttg tttatctatt cttcctaaaa attcaatttg accatcagat
     9421 gtataacgtg ctaaatcacc actatgatac aactttcctt taccaaatgg attattttgc
     9481 catttatcag ccattaattc tggacgatta atatatccta tcgctaaact atcacctgca
     9541 atacacaact cgcctggcat accaataccg cataacaaac catctgacat aatatacact
     9601 tggatgttag ataagggttt gccaattgga atcgtctcag gtatcaaatc accacaatga
     9661 tgtgaccaat acgatgtgat gactgttgac tcagatggtc cataggcatt gaaatacgtg
     9721 ccacaatgct tctcaatata tttaacaaag gatgccgtac tagttgcccc gcctgtaatc
     9781 aacttttcaa tataaaagtc ttccataaca ctacacatct gtaacggaat cgacgcaacc
     9841 gtcacacgat gcttattaat gagttgttgt aactgttctg gattaacacg ttcctctcta
     9901 tctggaatca caagcgtatg accatttaac aaacaacaat aaatctccat aactgatgca
     9961 tcaaaaacaa tatttgcatg ttgcaaaaat acttcattgt cgcctaattg caattcagtt
    10021 gaccatgcat gcactaaatt caacaaattt cgttgtcgta tggcaacccc tttaggcatc
    10081 ccggtcgtac cagatgtgta aatagcatac atctcattat ctaacatcgc tgtgttttca
    10141 agttgattgc catgtaaatc atcatattgt tcattttcct ttgattcaac aaagccttta
    10201 gcattttcca attgaatgac tggtaatgtc gtttcaattt caacgccgta agacatgact
    10261 gcagttactt tagcatcctc caaaattgca ccttgtcgtt tattcggaaa atcaatatcg
    10321 ataggtatat aagatgcacc tactttaact gtcgccaaca tcgccgcaat catttcaaaa
    10381 ctacgttctg taaacaaggc aacccgttga ccattgccca caccatttga taggagcatg
    10441 tgcgcaatgg catccacata gttgcgtaat gtttcatacg tcattgtcaa atcattcatg
    10501 actagcgcaa catgattacc ttgtcgtgag acaacttcat taaagtaact tatgatagat
    10561 ttatttcccg ggacattaag cattcgatcg ttaacatgcg tattgaccca atttagaagt
    10621 tcctccgtgc cgtttggtat atcacaaatt tgtagtgtat cttgatgctt caaaatataa
    10681 tcaatcataa tcatacattg attacccatg tgacgaactg tttctgagtg atataaatcg
    10741 gtattatact cgatattgat tgtatagtca tcgcgatctt cttcaatgat gaaagataaa
    10801 tcaaatttcg ccgtcactga tttgggttga atgtgtgtta atttactatg cccaaaatga
    10861 gcatgattcg tttcattgtt ttgtagtact aacatgacat caaataatgg attccgtgag
    10921 gcatcatgtg attgatctaa gtcatttact aaacattcga atgggtattc ttgatgctcg
    10981 tatgcctcca aactcatttc cttaacctct tgtaaaaact gtgtccacat tttatcaggt
    11041 gacggttgcc ctctatatac caacgtatta gcaaacatgc ctagcatttg ctccgtgcct
    11101 ttatgcatac gcgcactcat cacactaccg acaacaacat catcttttcg agcatatcta
    11161 cttaacaacg tcatgaccac actcataaag aacataaaat cagtaatttg atgcttttct
    11221 acatactttt gaagtagctg tctcatttgt tgattcattg taaatgacat cattgctcca
    11281 tttgtcgttt taatatttgg tctaacatag tctgtcggta agcttaaaat aggtacttca
    11341 tctttgaatt gagataacca atattgtcta tgtttcgtca tatcacgatg cgacatccac
    11401 tcactatagt ctttatattg caatttaagt ggtaacaata atttatgttg ataaagtgcg
    11461 ttaagatcat tcattaattg tatattactc ataccgtcat taatgatatg atgcgtatct
    11521 ataaagaggt atgcatgtaa gggacttcta atgtatctca ctctaatttg acttggcttt
    11581 tccaaattaa aaggtgctac aaattggcgc atgatttctt gttcatccgt aaaatgcgtg
    11641 ttaacttctt caaagtcaac tgcaacatct gccacaatac gttgtcgaac ctcatcatct
    11701 acaacaatat attgtgttcg taaaatctca tgtcgcgcta tcaaacgctg cactgcttgt
    11761 cgcaattgag ctacattaag ttctgatgat aaccgccata aaaaaggtac gttatacacc
    11821 gtatctttat ggtttgattt ccataataaa tacatacgct tttgtgcaga gctcagcaca
    11881 taatcatctt taactatagt ttctggaatc acttcatagt tttgttcttg aaccttagca
    11941 atcgcttgtg ctagttcaaa tacagttggc ttttgtaata aatcaccaat ttgtaatcgt
    12001 ttcccagtag atgcctctat ccgattcacc actaacgttg cttttaatga atggccacct
    12061 aattcaaaga aattatcatg aatacctact tgattcacat gtaaaatatc tgcaaaaatt
    12121 tggcatagca agtgttcggt atctgtactc ggtgctacat aggcatccgt atcgacatag
    12181 tccatgatag gcaatgcctt cttatctaat ttcccattaa tagtaatagg aatttgctca
    12241 atatgcatga aattaactgg tatcatgtac tccggtaagg tcatacgtaa ttgtgattta
    12301 atcttattat gtgataatgt atgcatcgct tcataataag caacgatata ctgatcttga
    12361 tcatgatttt gaacaataac aactgcttta ttaatacctt gtatacgctc gagcgcatgc
    12421 tcaacctctg acaactcaat cctaaaccct cgaatcttaa cttgtttgtc ctttcgatat
    12481 aaataatcta tgttgccatc gggtaacaaa cgaacgatat caccacttct atacatcagc
    12541 tgatttatat ttgaatcttt gataaattta tctgctgtca attctggctg atttaaataa
    12601 cctgcagcta acccaaagcc acttgtacat aattctccag gaataccaac gccacaccga
    12661 cgctcgcctt gcatgatata aacatgagta cccagaatcg gtttaccaat aggaatacga
    12721 tttggaactt tgttaggtat attatacgtc gttgtaaatg ttgtattttc agttggtcca
    12781 taaccattaa taatttgagg atgcttcggt ttttgattaa gcaaatccac ccacttagca
    12841 ttcaatactt ctccaccaat taataaatac tttaacggta ccaatacttc tattcgttca
    12901 ctagcaatct gattaaataa tgaggaggtt aaccacatag tattaacgtc attttcattg
    12961 attaattgtt ctaccgctat tggatttaat aattgttctt ttttagcaac aatcagcttt
    13021 ccaccattga gcaatgcacc atatatttca aatgttgcag catcaaaggc tatagttcct
    13081 gataacaaaa tcgtcgtctc ttcatttaat ggtacataat gattttgatg gaccaatcga
    13141 acaatacctc ggtgcggaat tagtgtccct ttagggttac cagttgtccc cgacgtgtaa
    13201 ataacataag catgatcttc taacgtgtta catttagaaa gattatcaat atttttccac
    13261 gctatcttat tcaaatcaat gtgattaata ttttgtttac cattttcata taaagcttgg
    13321 tacgttatta caactttagg cgttacatct tttaaaatgt actcctgacg atcacttgga
    13381 tagttcggat caattggcac gtaagcccca ccagctttca acacacctat catcgctatt
    13441 atcatctcaa tacttttttc agctatgaca gcgacacgat cattaggttc aacaccatac
    13501 tggtttctca aacggtgtgc taaatcattc gcgcgtgcat tcaatgtttg atatgttata
    13561 aacactccgt caaattgcac agcgacatga ttcggcgttg cttcaacttg ttgctcaaat
    13621 aaggtaacaa ctgtttgcgc atcatctatc tcaggcaaac ttaaattgat atcgtcatat
    13681 aattgaatat cacgttctgt catcaaatta agttcatcta cagttgttcg tttatttcca
    13741 ttttcttcag taatttgcaa ataaatattt cgaactaagt cactcagcgt ctcgattgag
    13801 agcaaatcat atgctgcact gttatatata attttgaacc cgtgttgatg tggaaaaatt
    13861 tcaatatccg ctaaagatgt atgtgcatca tgtatttgat gtacatcctc aataacatca
    13921 ttacaacaag acatcatatg atgataacaa tgaaaaatag tctctagtga aagcgaagac
    13981 ttcgcgcact gtaattgcga catattttgc aacacacatt tattaaaatc tgttgtaaaa
    14041 cgttgacata catcttttgc atcgattgtt aacgttaacg gcacaatatt tccgtgtaaa
    14101 tcatttggta aatgtgatgg tacatgtata cctaatgtga catcatgttg ttgactcatt
    14161 atatgattag ctaaatacac actaacagcc aacgatgcca tatctattga tgtcatatca
    14221 tcaatcaaat acgtttgata taaagcttgt tcaaatggat gcttaattgg aaaataacta
    14281 tcaatatgga tgtcagagtt attctctaac cgaaaatagt ttgagtctaa tgctatatgc
    14341 gatgcatctt gattgtcttt atcatcattt ctatttatat gtgcatgctg tcgagtattg
    14401 ttaataacag tattgccacg atatgcattg cataaatcat caagaaaaat atcaatttga
    14461 ctatcatcga aaatggacac atgaaaatct aatagtatat atgcagcatc agcgaactga
    14521 aacaatttaa ctttgaataa aggtgaatca ttaaaatggt aagtacttaa ttcttgctta
    14581 aaaaaagctt ctaaatcata gtttgcggaa gaagatggaa cttgttttat ctcaataaaa
    14641 ggcagaaatt catgaagtat cattgttaaa ttgtcatcgg tagtaacatc aaaaaaatgt
    14701 cttatagatg catgttgttg cacaattgtc gataatgcat acatcatttt agtagcttca
    14761 acatttttag cgagtttaac ccaatacgca ttacggtgtg tcgttgattc tgtattattt
    14821 ttgtatatac gaaaatattc ctgttgaaat ctcaaattac ccataatcat aaaaagtcct
    14881 tctttcatat cataatactc attacttact gaaattgcat gatgatatga taaccgacga
    14941 aatgttaatt aactcgttat gtaatgatta atataaaaca ccattcgcaa catatgagcg
    15001 atatattcta ccctaaaata catcttgtat catcgttaca attggtatat ttttcaatgt
    15061 aaattacata catcttcgat aaatagcaca ctacaactcg ttaatcactt tctgttgttc
    15121 acatctcatt gcaaactcaa tattgttgtt acaaaatatc catgaagcaa gtttatatta
    15181 aacaaacaac tcgcataaaa caattgttat ccttaaattt taacaaattc ttaataaatt
    15241 tatctctatt ttaattacga ccaaattaat aggttttcca tataaaaaga tgcataaaat
    15301 aaatatttaa ataaattcaa tttgtattca cttgtttttg tcccccaaat acaccagcaa
    15361 caagcatgct agcaccaatt gttaaaacga taaacatata cagtcccatt tgtaatgacg
    15421 ttaagaaaac acccaacaca atccctaacc tagctagtgt ttctgaaaaa tgaataccta
    15481 atgcattaac tgcactatat gttcctcttt tagctttagg aataatttta aagcgttgtt
    15541 ctgaaactat aggcgaataa ataatttcac ctacagtcgc aattatcata aaaacaacta
    15601 ataagccaaa ctgattaaga taggttagac cactatagcc aacaatataa atcagcaaac
    15661 cagtgattaa agctttttta aaatctattt tcaatacaac tttcgagatt gaatacgtga
    15721 gtaaaatgac gacgaccgta ttaatcatta gcaagattgc taacatctta gcacctgtaa
    15781 tatcatatga acctatactt attgtttcaa actgatcctt tagtctaata gcaatatatg
    15841 aggagattga aaattcaccc atcatgatga tactgaaccc cgaaatcaat aacatataat
    15901 tacggtcttt caaaactaat ttataactgc gaaatatatt cattatttgt aatttttgat
    15961 aacgacttgc atgcctcttg tcatcacttt gctttacttg atttcggtct tgaggtaacc
    16021 aaatatataa aataaagagt acaattaaaa atatacaagc tgctattaag aaaagtagta
    16081 acatactgta gccatacatc aagccaccta acaatgcccc aatagctacc gataagtttg
    16141 tcatccaata gctaatcttg taaatataat gttccacgtc ttcggtaatt gcatccataa
    16201 ttaatgtgtc cataactgga aattgtaatc cccaaacgat tgtaaatatg gcatatgcaa
    16261 cacaaaaacc aataatttgc cacaattgat gtgacccaaa tacgcccatg aacacaagca
    16321 ttatcaccat cgtcgcttga taaataagta ctagcaactt tttcggaaat atctcaataa
    16381 ggtaaccaga tataatggac aatggaaatt ttagaaccac taaaccaaca agatatatac
    16441 cgacaattga ttgacttaac atatctgtta aatatagtgc tataaacggt ataaatgctg
    16501 tcgtaataat tagctgtaaa aaattgctaa tcaatcgtac tttcaaagtc gtacttaagc
    16561 gtttcattaa taactacacc cctttccaca tatatacaca ctaccattta tataaaatct
    16621 attattagat tatacgttga aacaaagaat ttttagttat acaaagttat ttaacaaaca
    16681 aatgcatcga ctttacaatg catcttgcat ataatgcctc aaaaaaatta gcactatatg
    16741 actatatatt attccaaatg aatgaaattt atcaataatt gcgtaagtga acacatatgt
    16801 gcgtaaatgt taaatcattg tcctaacata gctgtgacta tacttagatg taatcataaa
    16861 aaaactactg ccatctttac aacagcagta gtgcttatat gattcattta tataaaattg
    16921 aaagcgttaa tcgctagccc agcatcctta ttctatttag ctgtataact tttactagca
    16981 atacgaccac ttgcaacaat aatatcttta tcttggaaag gttcatgatt gaagaaacgc
    17041 tctaaaatat ctttaactcc atcttcaata cgtttttgtg cttctaaagt catacctgaa
    17101 tagtgaaccg tcatagcatt tctaggcatt gttctccatg gatgatcagc aggtgcaggt
    17161 tgtggatacc aaacatcacc agcatatcct tgtaaatgct cggatgctaa cgcttcaact
    17221 aacgcatcgc gatttacaat tttaccacgt gcagtattca ctaaataact gtgttttttc
    17281 atacgactta aaacatcttt atcaaataag ttatcagttt ctggtgttaa tggtgcatga
    17341 attgtaatcg catcacttgt tgaaacaagt tcatcaaagc ttacaaattt agacaattta
    17401 tggtcttgtt gattgattgg atcatagtgt tgtaatgtta cattaaatgg cgctaatctt
    17461 tcagcaacaa gttgtccaat tcgaccaaat ccaaaaatac caattgtttt gtgttgtaat
    17521 tcatgcgcat gattacctac ttgagacaag ttccattcac cttctactga ttgacgatga
    17581 ccttcttcat agtttctaag aagtattaat aaatccataa ccgcatgttc tgccacacta
    17641 actgtattac ttcctgtaac ttcaacgaca ccaatattgt gttcacttgc tgccgctaaa
    17701 tctacatggt cagatcctac acctgctgta attgctaatt tcaagttcgg tgctttttca
    17761 atacgttcac gagtcatata tgcaggataa aatggcgcac taataatcac atccatatct
    17821 ggtaaatgtt tatctaagtc ttcaccatta tctgctaata taatgaactc atgtcctctt
    17881 tcctctaaaa atgtttttaa tcctaatgct tttttagtat taagtaattg attttcttga
    17941 ccttctactg cttctggaaa taatgctacg attttcatat ccatctctcc taaatgataa
    18001 attttgactt ccttaaagta cgtgttgcat gttgcttgtt ttaaaaatat gacaaaaaaa
    18061 cggcaccgtt tgacatacca cccccttgaa ttattgttaa attcaaagcg aagttagcaa
    18121 acagcaccgt gtaccttaca ccagcaacac ttattctatt gacttatcga aaacaaatat
    18181 tgaaatgact tcatcatcat tcaaatcaat atctgtaaat aattttacaa acttagcgcc
    18241 tacaagactt tccatctcat ttacatctat ttctttatac atctgtttaa tcttctctgt
    18301 gcgtgtataa tggagcatcg attcattgcg tttgtcgttt aggtaaaaac tctcaacttt
    18361 actcaaaaca cctgtcatat gtgcaatcgc ccaattatct ttaaacgaca ctcgaatact
    18421 attgggtcct ttacccacgt attctttacg ataagatctt actagattcg agaattgttg
    18481 ttctttagta tgcgtatcta tagtattcac cttctttaaa ataagaagtt cattaattgt
    18541 tcacattttc attatacacc ttattaacac tttttcaatg ataacgatta ttattattac
    18601 gaaatgtata ttttcctagt taaaaggata taacaaatta acagaaaatg tttataaact
    18661 aagatttata ttattttaaa aaatgtttca acaaacgcat ctaaacaaaa aatcagctta
    18721 tcacacttac cccttcaact ctgcttcaag tttacctaaa tgtcttaatg tcggtgtcaa
    18781 tgctgcgaag aagaatcctt cttttaac
//
LOCUS       DALAGD010000061        18675 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.062, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000061 DALAGD010000000
VERSION     DALAGD010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18675
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.062"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            252..743
                     /gene="lspA"
                     /locus_tag="O2701_001718"
     CDS             252..743
                     /gene="lspA"
                     /locus_tag="O2701_001718"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCZ4293606.1"
                     /translation="MHKKYFIGTSILIAVFVVIFDQVTKYIIATTMKIGDSFEVIPHF
                     LNITSHRNNGAAWGILSGKMTFFFIITIIILIALVYFFIKDAQYNLFMQVAISLLFAG
                     ALGNFIDRILTGEVVDFIDTNIFGYDFPIFNIADSSLTIGVILIIIALLKDTSNKKEK
                     EVK"
     gene            743..1660
                     /locus_tag="O2701_001719"
     CDS             743..1660
                     /locus_tag="O2701_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCZ4293607.1"
                     /translation="METYEFNITDKEQTGMRVDKLLPELNNDWSRNQIQDWIKAGLVV
                     ANDKVVKSNYKVKLNDHIVVTEKEVVEADILPENLNLDIYYEDDDVAVVYKPKGMVVH
                     PSPGHYTNTLVNGLMYQIKNLSGINGEIRPGIVHRIDMDTSGLLMVAKNDIAHRGLVE
                     QLMDKSVKRKYIALVHGNIPHDYGTIDAPIGRNKNDRQSMAVVDDGKEAVTHFNVLEH
                     FKDYTLVECQLETGRTHQIRVHMKYIGFPLVGDPKYGPKKTLDIGGQALHAGLIGFEH
                     PVTSEYIERHAELPQDFEDLLDTIRKRDA"
     gene            2059..2586
                     /gene="pyrR"
                     /locus_tag="O2701_001720"
     CDS             2059..2586
                     /gene="pyrR"
                     /locus_tag="O2701_001720"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HCZ4293608.1"
                     /translation="MSERIIMDDAAIQRTVTRIAHEILEYNKGTDNLILLGIKTRGEY
                     LANRIQDKIHQIEQQRIPTGTIDITYFRDDIEHMSSLTTKDAIDIDTDITDKVVIIID
                     DVLYTGRTVRASLDAILLNARPIKIGLAALVDRGHRELPIRADFVGKNIPTSKEETVS
                     VYLEEMDQRNAVIIK"
     gene            2804..4111
                     /locus_tag="O2701_001721"
     CDS             2804..4111
                     /locus_tag="O2701_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCZ4293609.1"
                     /translation="MQNDEMFERTVKPVLDVNEKPQPAQWAFLSLQHLFAMFGATVLV
                     PFLTGLPISAALLASGIGTLLYILITKAQIPAYLGSSFAFITPIITGLSTHSLGDMLV
                     ALFMSGVMYVIIGILIKLSGTAWLMKLLPPVVVGPVIMVIGLSLAPTAVNMAMYENPG
                     DMKGYNISFLIVAMITLLVTIVVQGFFKGFLSLIPVLVGIIVGYVVAIFMGIVKFDAI
                     MSAKWIDFPHIYLPFKDYVPSFHLGLVLVMIPIVFVTVSEHIGHQMVLNKIVGRNFFE
                     KPGLDKSIIGDGVSTMFASIIGGPPSTTYGENIGVLAITRIYSIYVIGGAAVIAIVLA
                     FIGKFTALISSIPTPVMGGVSILLFGIIAASGLRMLVESKVDFANNRNLVIASVILVV
                     GIGNLVFNLKEIGINLQIEGMALAALSGIILNLILPKEKKQNN"
     gene            4139..5020
                     /locus_tag="O2701_001722"
     CDS             4139..5020
                     /locus_tag="O2701_001722"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HCZ4293610.1"
                     /translation="MNHLLSMEHLSTDQIYKLIQKASQFKSGERQLPNFEGKYVANLF
                     FENSTRTKCSFEMAELKLGLKTISFETSTSSVSKGESLYDTCKTLESIGCDLLVIRHP
                     FNNYYEKLANINIPIANAGDGSGQHPTQSLLDLMTIYEEYGYFEGLNVLICGDIKNSR
                     VARSNYHSLKALGANVMFNSPNAWIDDSLEAPYVNIDDVIETVDIVMLLRIQHERHGL
                     AEETRFAADDYHQKHGLNEVRYNKLQEHAIVMHPAPVNRGVEIQSDLVEASKSRIFKQ
                     MENGVYLRMAVIDELLK"
     gene            5038..6312
                     /locus_tag="O2701_001723"
     CDS             5038..6312
                     /locus_tag="O2701_001723"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCZ4293611.1"
                     /translation="MKLIKNGKVLQNGELQQADILIDGKVIKQIAPAIEPSNGVDIID
                     AKGHFVSPGFVDVHVHLREPGGEYKETIETGTKAAARGGFTTVCPMPNTRPVPDSVEH
                     FEALQKLIDDNAQVRVLPYASITTRQLGKELVDFPALVKEGAFAFTDDGVGVQTASMM
                     YEGMIEAAKVNKAIVAHCEDNSLIYGGAMHEGKRSKELGIPGIPNICESVQIARDVLL
                     AEAAGCHYHVCHVSTKESVRVIRDAKRAGIHVTAEVTPHHLLLTEDDIPGNNAIYKMN
                     PPLRSTEDREALLEGLLDGTIDCIATDHAPHARDEKAQPMEKAPFGIVGSETAFPLLY
                     THFVKNGDWTLQQLVDYLTIKPCETFNLEYGTLKENGYADLTIIDLDSEQEIKGEDFL
                     SKADNTPFIGYKVYGNPILTMVEGEVKFEGDK"
     gene            6314..7414
                     /gene="carA"
                     /locus_tag="O2701_001724"
     CDS             6314..7414
                     /gene="carA"
                     /locus_tag="O2701_001724"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCZ4293612.1"
                     /translation="MQSKRYLVLEDGSFYEGYRLGSDNLTVGEIVFNTAMTGYQETIS
                     DPSYTGQIITFTYPLIGNYGINRDDFESLVPTLNGIVVKEASAHPSNFRQQKTLHDVL
                     ELHQIPGIAGVDTRSITRKIRQHGVLKAGFTDRKEDIDQLVKHLQQVELPKNEVEIVS
                     TKTPYVSTGKDLSVVLVDFGKKQNIVRELNVRGCNVTVVPYTTTAEEILAMAPDGVML
                     SNGPGNPEVVECAIPMIQGILGKIPFFGICLGHQLFALSQGASSFKMKFGHRGANHPV
                     KNLETGKVDITSQNHGYAIDIDSLKSTDLEVTHLALNDGTVEGLKHKTLPAFSVQYHP
                     EANPGPSDSNYLFDDFVAMMTNFKEKERHINA"
     gene            7407..10580
                     /gene="carB"
                     /locus_tag="O2701_001725"
     CDS             7407..10580
                     /gene="carB"
                     /locus_tag="O2701_001725"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCZ4293613.1"
                     /translation="MPKRNDIKTILVIGSGPIIIGQAAEFDYAGTQACLALKEEGYRV
                     ILVNSNPATIMTDKEIADKVYIEPLTHDFIARIIRKEQPDALLPTLGGQTGLNMAIQL
                     HESGVLQDNNVQLLGTELTSIQQAEDREMFRTLMNDLNVPVPESDIVNTVEQAFKFKE
                     QVGYPLIVRPAFTMGGTGGGICHNDEELHEIVSNGLHYSPATQCLLEKSIAGFKEIEY
                     EVMRDKNDNAIVVCNMENIDPVGIHTGDSIVVAPSQTLSDVEYQMLRDVSLKVIRALG
                     IEGGCNVQLALDPHSFDYYIIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     LNPITGTSYAAFEPTLDYVISKIPRFPFDKFEKGERELGTQMKATGEVMAIGRTYEES
                     LLKAIRSLEYGVHHLGLPNGESFDLDYIKERISHQDDERLFFIGEAIRRGTTLEEIHN
                     MTQIDYFFLHKFQNIIDIEHQLKEHQGDLEYLKYAKDYGFSDKTIAHRFNMTEEEVYQ
                     LRMENDIKPVYKMVDTCAAEFESSTPYYYGTYETENESIVTDKEKILVLGSGPIRIGQ
                     GVEFDYATVHAVWAIQKAGYEAIIVNNNPETVSTDFSISDKLYFEPLTEEDVMNIINL
                     EKPKGVVVQFGGQTAINLADKLAKHGVKILGTSLENLNRAEDRKEFEALLRKINVPQP
                     QGKTATSPEEALANAAEIGYPVVVRPSYVLGGRAMEIVDNDKELENYMTQAVKASPEH
                     PVLVDRYLTGKEIEVDAICDGETVIIPGIMEHIERAGVHSGDSIAVYPPQTLTEDELA
                     TLEDYTIKLAKGLNIIGLINIQFVIAHDGVYVLEVNPRSSRTVPFLSKITDIPMAQLA
                     MRAIIGEKLTDMGYQEGVQPYAEGVFVKAPVFSFNKLKNVDITLGPEMKSTGEVMGKD
                     TTLEKALFKGLTGSGVEVKDHGTVLMTVSDKDKEEVVKLAQRLNEVGYKILATSGTAN
                     KLAEYDIPAEVVGKIGGENDLLTRIQNGDVQIVINTMTKGKEVERDGFQIRRTTVENG
                     IPCLTSLDTANALTNVIESMTFTMRQM"
     gene            10690..11382
                     /gene="pyrF"
                     /locus_tag="O2701_001726"
     CDS             10690..11382
                     /gene="pyrF"
                     /locus_tag="O2701_001726"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCZ4293614.1"
                     /translation="MKDLPIIALDFESKEKVNQFLDLFDESLFVKVGMELFYQEGPQL
                     INEIKERGHDVFLDLKLHDIPNTVGKAMEGLAKLNVDLVNVHAAGGVKMMSEAIKGLR
                     KHNQDTKIIAVTQLTSTTEDMLRHEQNIQTSIEEAVLNYAKLANAAGLDGVVCSPLES
                     RMLTEKLGTSFLKVTPGIRPKGASQNDQHRITTPEEARQLGSTHIVVGRPITQSDNPV
                     ESYHKIKESWLV"
     gene            11382..11993
                     /locus_tag="O2701_001727"
     CDS             11382..11993
                     /locus_tag="O2701_001727"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCZ4293615.1"
                     /translation="MAKEIAKSLLDIEAVTLSPNDLYTWSSGIKSPIYCDNRVTLGYP
                     LVRGAIRDGLINLIKEHFPEVEVISGTATAGIPHAAFIAEKLKLPMNYVRSSNKSHGK
                     QNQIEGAKSEGKKVVVIEDLISTGGSSVTAVEALKLAGAEVLGVVAIFTYGLKKADDT
                     FSNIQLPFYTLSDYNELIEVAENEGKISSEDIQTLVEWRDNLA"
     gene            12023..12235
                     /locus_tag="O2701_001728"
     CDS             12023..12235
                     /locus_tag="O2701_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293616.1"
                     /translation="MNDKTSNDLYGKIKHCNEFINHSNDSNLSSSHDVDESSTKQKHI
                     KNKTTIDHNDDLFKHVKDILRKQEQI"
     gene            12672..13073
                     /locus_tag="O2701_001729"
     CDS             12672..13073
                     /locus_tag="O2701_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCZ4293617.1"
                     /translation="MDIPKITTFLMFNNQAEEAVKLYTSLFEDSEIITMAKYGENGPG
                     DPGTVQHSIFTLNGQVFMAIDANSGTELPMNPAISLFVTVKDTIEMERLFNGLKDEGA
                     ILMPKTNMPPYREFAWVQDKFGVSFQLALPE"
     gene            complement(13336..15033)
                     /locus_tag="O2701_001730"
     CDS             complement(13336..15033)
                     /locus_tag="O2701_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HCZ4293618.1"
                     /translation="MAYDGLFTKKMVESLQFLTTGRVHKINQPDNDTILMVVRQNRQN
                     HQLLLSIHPNFSRLQLTTKKYDNPFNPPMFARVFRKHLEGGIIESIKQIGNDRRIEID
                     IKSKDEIGDTIYRTVILEIMGKHSNLILVDENRKIIEGFKHLTPNTNHYRTVMPGFNY
                     EAPPTQHKINPYDITGAEVLKYIDFNAGNIAKQLLNQFEGFSPLITNEIVSRRQFITS
                     STLPEAFDEVMAETKLPPTPIFHKNHETGKEDFYFIKLNQFNDDTVTYDSLNDLLDRF
                     YDARGERERVKQRANDLVRFVQQQLYKYQNKLAKLIEEYEQSKNKDTEQLYGELITAN
                     IYRIKQGDKEVTALNYYTNEEVVIPLNPTKSPSANAQYYYKQYNRMKTRERELQHQIQ
                     LTKDNIDYFSTIEQQLHHISVHDIDEIRDELAEQGFMKQRKNQTKKKKAQIQLQHYVS
                     TDGDDIYVGKNNKQNDYLTNKKAKKTHTWLHTKDIPGSHVVIFNDAPSDTTIKEAAML
                     AGYFSKAGNSGQIPVDYTLIKNVHKPSGAKPGFVTYDNQKTLYATPDYELIQKMKQS"
     gene            15308..15931
                     /gene="gmk"
                     /locus_tag="O2701_001731"
     CDS             15308..15931
                     /gene="gmk"
                     /locus_tag="O2701_001731"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCZ4293619.1"
                     /translation="MDNEKGLLIVLSGPSGVGKGTVRKRIFEDPSTSYKYSISMTTRQ
                     MREGEVDGVDYFFKTRDAFEALIKDDQFIEYAEYVGNYYGTPVQYVKDTMDEGHDVFL
                     EIEVEGAKQVRKKFPDALFIFLAPPSLEHLRERLVGRGTESDEKIQSRINEARKEVEM
                     MNLYDYVVVNDEVELAKNRIQCIVEAEHLKRERVEAKYRKMILEAKK"
     gene            15931..16149
                     /locus_tag="O2701_001732"
     CDS             15931..16149
                     /locus_tag="O2701_001732"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCZ4293620.1"
                     /translation="MLNPPLNQLTSQIKSKYLIATTAAKRAREIDEQPETELLSEYHS
                     FKPVGRALEEIADGKIRPVISSDYYGKE"
     gene            16365..17564
                     /gene="coaBC"
                     /locus_tag="O2701_001733"
     CDS             16365..17564
                     /gene="coaBC"
                     /locus_tag="O2701_001733"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCZ4293621.1"
                     /translation="MKKILLAVTGGIAAYKAIDLTSKLTQSGYEVRVMLTNHAQKFVT
                     PLAFQAISRNAVYTDTFIEENPSEIQHIALGDWADAIIVAPATANTIAKLSVGIADDL
                     VTSTLLATETPKFIAPAMNVHMYENKRTQQNINILKEDGYHFIEPGSGFLACGYVAKG
                     RMEEPLQIVSVIDAHFQNSNRLANSSFQDKRALVTAGPTIEVIDPVRFVSNRSSGKMG
                     YAIAEALRNRGAIVTLVAGPTTLEDPKDIEVIHVQSAEEMFEQVTSRFDEQDIVVKAA
                     AVSDYTPVDVLEHKMKKQDGDLSVSFKRTKDILKYLGEHKTSQYLIGFAAETEDIENY
                     AQQKLRKKNADVIISNNVGDMSIGFSSDDNELTMHFKNNEKVNIKKGKKVVLAAQILD
                     ELETRWQ"
     gene            17564..>18675
                     /locus_tag="O2701_001734"
     CDS             17564..>18675
                     /locus_tag="O2701_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCZ4293622.1"
                     /translation="MIAKVIVDVASKSVDYKFDYIIPEQLESVIQPGVRVIVPFGPRT
                     IQGYVMEVTAEPDAQLDVSKLKKIIEVKDIQPELTSELIALSEWMGSTHVIKRISMLE
                     VMLPSAIKAKYKKAFKMKDDIELPSALLQKFDKHGYYYYKDAQKNNDIQLLMKLLKDD
                     IVEEKTILTQNITKKTKRAVRVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFL
                     TDIEDMGFSKSSLDGLIKKGYVEKYDAVVERDPFKDRVFEQESKQQLTEDQYKAYEAI
                     KAKIVSQEQETFLLHGVTGSGKTEVYLQTIEDVLSQGKQAMMLVPEIALTPQMVLRFK
                     RRFGDDVAVLHSGLSNGERYDEWQKIRDGRARVSVGA"
BASE COUNT         6597 a         2708 c         3519 g         5851 t
ORIGIN      
        1 tttttaatta gaaaatatct tactactgtc tttttaggga tttatgttcc agcctgcttt
       61 cctaattttt aatgtcatct taaatatgat aaatgaataa ttaagttcat atttaatgtc
      121 aaaacatagt agtttatcaa gtattgagtt agtaacatta gatttaatgt aatatgctta
      181 ctttttttat tagcaggtgt aagctataat ataaagagtt gtctcatgga cgattgattg
      241 gaggaacgaa aatgcacaaa aaatatttta ttggcacttc cattttaata gcagtatttg
      301 tcgttatatt tgaccaagtt actaaatata ttatagctac tacaatgaaa attggagatt
      361 catttgaagt gataccgcac tttttaaaca taacatcaca tcgaaataat ggtgctgcat
      421 ggggaatatt gagtggaaaa atgacatttt tctttattat taccattatt atattaatag
      481 ccttagtata tttctttatt aaagatgctc aatataattt gtttatgcaa gttgctatta
      541 gtttactttt tgcaggtgca cttggaaact ttattgatag aattttaaca ggagaagttg
      601 ttgactttat tgatacaaat atttttggtt atgattttcc aatatttaat atcgcagatt
      661 caagtttaac aattggtgta atattaatta ttattgcctt attaaaggat acttccaata
      721 aaaaggagaa ggaggttaag taatggagac ttatgaattt aacattacag ataaagaaca
      781 aacaggtatg cgtgtagata agttgctgcc tgaattaaat aatgattggt ctcgtaacca
      841 gatacaagat tggattaaag caggtttagt cgttgcaaac gataaagttg ttaaatctaa
      901 ttataaagtg aaacttaatg atcatatagt tgtcactgaa aaagaagtgg ttgaagctga
      961 tattctacct gaaaatttaa atttagatat ttattatgaa gatgacgatg ttgcagttgt
     1021 atataaaccg aaaggcatgg tagttcatcc atcaccaggg cattatacca atacattagt
     1081 taatggttta atgtatcaaa ttaaaaattt gtcaggtatt aatggagaaa ttcgtccagg
     1141 tattgttcac cgtatagata tggatacttc tggtttatta atggttgcta aaaatgatat
     1201 tgctcatcgt gggcttgtag aacaattaat ggataaatct gttaaaagaa aatatatcgc
     1261 tttagttcac gggaatattc ctcatgatta cggtacaatc gatgcgccaa ttggtagaaa
     1321 caaaaatgat cgtcaatcta tggctgttgt tgatgatggt aaggaagcag tgacacattt
     1381 taacgtacta gaacatttta aagattatac gcttgttgaa tgtcaacttg aaacaggacg
     1441 tacgcatcaa atccgtgttc acatgaaata tattggcttc ccattagttg gtgatccaaa
     1501 gtatggaccg aaaaagacat tggatattgg tggtcaagct ctacatgctg gacttattgg
     1561 attcgaacat ccagtaacaa gtgaatatat tgaaagacat gctgaattac cacaagactt
     1621 tgaagattta ttagatacaa ttcgaaaaag agatgcataa ttgtgtcgtg ctataattac
     1681 gataacatta ttatgtaaac tataagtgtt tatcgttttc gtttttttaa atgaaaacac
     1741 attataaaat agattaaggt taacattttt actgagtatg gttaattttt caatatagat
     1801 tgacaaagcg cctgattagt attatcctta taacagttta agtacaacat aaagagtctt
     1861 taattgagcg tccagagaga cgtcaaagac atgaaaatat agatatatac taaaatgatc
     1921 ataacacttt cacatatgca agaataagta gtaatactac gattcttcta tgtaaatagg
     1981 ctgattgtat atactaaacc tcatgtcgat attaaagaca tgaggttttt tgtttctaaa
     2041 ttttgaaagg gtgagaatat gtctgaacgt atcataatgg atgatgccgc aatacaacgt
     2101 acagtgacga gaatcgctca cgaaattttg gagtataaca aaggtactga taatttaatt
     2161 cttttaggta tcaaaacaag aggtgaatat ttagcgaatc gtatacaaga taaaattcat
     2221 caaattgagc aacaacgtat acctactgga acaattgata ttacatactt tagagatgat
     2281 atagagcaca tgtcatcact tacgacaaaa gacgcaatag acatcgacac agatattaca
     2341 gataaagtag tcatcattat tgacgatgtg ctgtatactg gtcgaacggt tcgtgcttca
     2401 cttgatgcta ttttgctaaa tgctagacct attaaaattg gtttagctgc tttggttgat
     2461 cgaggacatc gtgagttacc aattcgagca gattttgttg gtaaaaatat acctacttct
     2521 aaagaggaaa cggtaagtgt ctatttagaa gaaatggatc aaagaaatgc agttataatt
     2581 aaataatacc ccttttaatt cagtacgaga gactgaaaaa ggtgggggag ttgaatgata
     2641 aataagtttt catatgcttg tttatctaat aataccctca tgttcgacaa ctataagtca
     2701 atttctatgg acgaaaatga cttgtagcaa cttgtcacac tttttgtctc tttacatata
     2761 ctgatgtaaa gagatttttt tattatcgga ggaaatgaat attatgcaaa atgatgaaat
     2821 gtttgaacga acagtaaaac ccgtactaga tgtaaatgaa aaaccacaac cagcgcaatg
     2881 ggcattttta agcttacaac atttatttgc gatgtttggc gcaacagtac tagtaccatt
     2941 cttaacagga ctaccaatat cagcagcgtt actagcttcg ggaatcggta cattacttta
     3001 tatcttaata acgaaggcgc aaataccagc atacttggga tctagctttg catttatcac
     3061 gccaattatc acgggattaa gtacgcatag cttaggagac atgcttgtag cattattcat
     3121 gagtggtgtt atgtacgtca tcatcgggat tctaatcaaa ttaagtggga cagcatggtt
     3181 aatgaaatta ttaccaccag ttgttgttgg accagtcatc atggtaattg gtttaagctt
     3241 agcgcctact gcagtcaata tggcaatgta tgaaaatcct ggagatatga aaggttacaa
     3301 tatcagtttc ttaattgttg caatgataac cttgcttgta acaatagtcg ttcaaggatt
     3361 ttttaaagga ttcctatctt taattccagt tcttgtaggt attatcgtag gttatgtggt
     3421 agcgattttt atggggattg tgaaatttga tgcaattatg tcagcaaaat ggatagattt
     3481 ccctcatatt tatctgccat ttaaagatta tgtaccttca tttcacttag gacttgtact
     3541 tgtaatgatt ccgattgtgt tcgtaacagt aagtgaacat attgggcacc aaatggtatt
     3601 gaataaaatc gtaggtagaa acttctttga aaagccagga cttgataaat caatcattgg
     3661 tgatggtgtt tctacaatgt ttgccagtat tattggtgga ccaccaagta caacatacgg
     3721 tgaaaatatc ggtgtattag cgattaccag aatatacagt atttacgtca ttggtggtgc
     3781 agcagttata gcaattgttt tagcattcat tggtaagttc actgcattaa tttcttctat
     3841 acctacacca gttatgggag gagtatctat attacttttc ggtattattg cagcaagtgg
     3901 cttaagaatg ttagttgaaa gcaaagtaga ttttgcgaac aatcgaaatt tagttatagc
     3961 ttctgtaatt ttagttgtag gtatcggtaa tttagtattt aacttaaaag aaattggtat
     4021 caaccttcaa attgagggga tggcattagc tgcactttca ggaattattt tgaacttaat
     4081 cttacctaaa gagaaaaaac aaaacaatta agatttacaa attaaggagg gcgcttttat
     4141 gaatcattta ttatcaatgg aacatttatc tacagatcaa atatacaaac ttatccaaaa
     4201 ggcaagtcaa tttaaatctg gtgaacgtca actaccaaac tttgaaggga aatatgtcgc
     4261 aaatttattc tttgaaaatt ctactcgaac aaaatgtagt tttgaaatgg cagaacttaa
     4321 gctagggtta aaaacgatta gctttgaaac atcaacatca tctgtttcaa aaggtgaatc
     4381 tttatatgac acatgtaaaa ctttagaaag tattggctgt gatttattag tcattagaca
     4441 tccgtttaat aactactatg aaaaattagc gaatattaac atcccaattg cgaatgctgg
     4501 tgatggtagt ggacaacatc caacacaaag tttacttgat ttaatgacga tatatgaaga
     4561 atatggatat tttgaaggct tgaatgtatt gatttgtgga gacattaaaa attcacgtgt
     4621 cgcacgtagt aattaccata gtttaaaagc attaggtgca aacgtaatgt ttaatagccc
     4681 aaatgcttgg attgatgatt ctttagaagc accttatgta aatatagatg atgttataga
     4741 aacagtagat atagttatgt tattaagaat tcaacatgaa aggcatgggc ttgcagaaga
     4801 aactagattt gcagcagatg attatcatca aaagcatggc ttaaatgaag tacgctataa
     4861 caaattacaa gaacatgcta ttgttatgca tccggcacct gtgaatagag gagtagaaat
     4921 acaaagcgat ttagtagaag cttcaaaatc aagaattttt aagcaaatgg aaaatggcgt
     4981 ttacttaaga atggcagtca ttgatgaatt attaaaatag gtaaggggac gaaaatgatg
     5041 aaattaatta aaaacggtaa agtattacaa aatggcgaat tacaacaagc agatatttta
     5101 attgatggta aggtaattaa acaaattgca cctgcaattg aaccaagcaa tggtgttgac
     5161 atcatagatg cgaaaggtca ctttgtgtca cctggatttg tcgatgttca tgttcattta
     5221 cgtgaacctg gtggtgaata taaagagaca attgaaactg gtactaaagc tgctgctaga
     5281 ggcggattta caactgtatg tccaatgcct aacacaagac cggtaccaga ttctgtagaa
     5341 cattttgaag ctttacaaaa attaatcgat gacaatgctc aagtacgtgt attaccttat
     5401 gcttcaatta caacacgtca attaggtaaa gaattggttg atttcccagc actagtaaaa
     5461 gaaggtgcct ttgcgtttac agatgacggt gtaggagtac aaactgcaag catgatgtat
     5521 gaaggcatga ttgaagctgc aaaagtaaac aaagccatcg tagcacactg tgaagataat
     5581 tcattaatct atggtggtgc aatgcatgaa gggaaacgca gtaaagagtt aggtatacca
     5641 ggtattccaa acatttgtga atctgttcaa atcgcaagag atgtactatt agctgaagca
     5701 gcaggttgtc attatcatgt atgtcatgtt tctactaaag aaagtgttag agtcattcgt
     5761 gacgctaaac gcgcaggcat tcatgttaca gctgaagtta caccacacca tttattgtta
     5821 acagaagatg atattcctgg taataatgcc atttataaaa tgaatccacc attgagaagt
     5881 actgaagata gagaggcttt gttagaaggg ttactagacg gtacaattga ctgtatcgca
     5941 acagaccatg caccacatgc acgtgatgaa aaagcacaac caatggaaaa agcaccattc
     6001 ggaattgttg gtagtgaaac agcattccca ttattatata cgcattttgt aaaaaatggt
     6061 gattggacat tacaacaatt agtagattac ttaacaatta aaccatgtga gacatttaat
     6121 ttagaatacg gcacattaaa agaaaatggt tatgcagatt taacaatcat tgatttagat
     6181 agtgaacaag aaattaaagg agaagatttc ttatcaaaag cagataatac accatttatc
     6241 ggctataaag tttatggaaa tccgatctta acaatggttg aaggcgaagt taaatttgag
     6301 ggggataaat aatatgcaaa gcaaacgtta tctagtgtta gaagacggtt ctttttacga
     6361 aggctaccgt ttaggatctg ataacttaac tgtaggagaa attgtattta atacagcgat
     6421 gacaggttat caagaaacta tttcagatcc atcatataca ggtcagatca ttacttttac
     6481 gtatccatta atcggtaatt atggtatcaa tagagacgat tttgaatcat tagtacctac
     6541 attaaacggt attgtagtga aagaagcgag tgcgcatcca agtaatttta gacagcaaaa
     6601 gacacttcat gacgttttag aattgcatca aattccaggg attgcaggtg ttgatacaag
     6661 aagtattacg cgtaaaattc gacaacacgg tgtgttaaaa gctggtttta ctgatcgaaa
     6721 agaagatatt gatcaacttg tcaaacattt acaacaagta gaattaccta aaaacgaagt
     6781 agaaatcgtt tcgactaaaa caccgtatgt ttcgacaggt aaggatctaa gtgtcgtact
     6841 tgtagacttt ggtaagaagc aaaatattgt tcgagaatta aacgtcagag gttgtaacgt
     6901 cacagttgta ccatatacaa ctactgccga agaaatttta gcaatggctc cagatggcgt
     6961 tatgctatca aacggaccag gtaatcctga agttgtagaa tgtgcgattc caatgattca
     7021 aggaatttta gggaaaattc cgttctttgg tatctgtcta ggacatcaac tttttgcatt
     7081 atctcaagga gcaagctcat ttaaaatgaa gtttggtcat cgtggtgcga accatccagt
     7141 taaaaattta gagactggaa aagttgatat tacgagtcaa aaccatggat atgcaataga
     7201 tatagattcg ttaaaaagta ctgatttaga agttactcat cttgcattaa atgatggtac
     7261 tgtagaaggt ttaaaacata aaacattacc agcattttct gttcaatacc atcctgaagc
     7321 aaatccagga ccgtcagatt caaactatct atttgatgat tttgtagcaa tgatgactaa
     7381 ttttaaggaa aaggagcgtc atatcaatgc ctaaacgtaa tgatatcaaa acaattttag
     7441 taatagggtc tgggccaatt atcataggtc aagcagctga atttgattat gctggaacac
     7501 aagcatgtct agctttaaaa gaagagggat atcgagttat tcttgtaaat tcaaatccag
     7561 cgacaatcat gactgataag gaaattgcgg ataaagtata tatcgaaccg ttaactcatg
     7621 attttatagc gcgaattata cgtaaagagc aacctgacgc tttacttcca actttaggtg
     7681 gtcaaacagg tttaaacatg gcgattcaac tacacgaaag tggtgtgctt caagataata
     7741 acgtccaatt attaggaact gagctaacat caattcaaca agcagaagac cgtgaaatgt
     7801 ttagaacatt aatgaatgat ttaaacgttc ctgtaccaga gagtgacatt gtaaatacag
     7861 tagagcaagc ctttaaattc aaagagcaag tgggataccc gctaattgtt agaccggcat
     7921 ttacgatggg tggtaccgga ggcggtattt gtcataatga tgaagaatta catgaaatcg
     7981 tctcaaatgg tcttcattat agtccagcaa cgcaatgttt attagaaaaa tctatcgcag
     8041 gttttaaaga aatcgaatac gaagtaatgc gtgataaaaa cgataatgcc atcgttgtat
     8101 gtaacatgga aaatattgat ccagttggta ttcatacagg cgattcaatt gttgtggctc
     8161 ctagtcaaac attatcagat gttgagtatc aaatgttacg tgatgtttca ttaaaagtta
     8221 ttcgagcttt aggtatcgaa ggtggttgta atgttcaatt agcattagat ccccattcat
     8281 tcgattatta tattatagaa gtaaatccgc gtgtatcacg ttcatcagcg ttagcttcaa
     8341 aagcaacagg atatcctatt gcaaaattag ctgctaaaat cgcggttggt ctaacattag
     8401 atgaaatgtt aaatccaatt acaggaacat cttatgcagc gtttgaacca actttagact
     8461 atgtgatttc aaaaatacca agatttcctt ttgataaatt tgaaaaagga gaacgagagc
     8521 ttggcacaca aatgaaagca acaggtgaag ttatggccat tggtcgaact tacgaagaat
     8581 cattgttaaa agcaattcga tcacttgagt atggtgtgca tcacttagga ttaccaaatg
     8641 gtgaaagctt cgatcttgat tatattaaag aacgtatttc acaccaagat gatgaacgat
     8701 tatttttcat cggcgaagca attagaagag gcacaacatt agaagaaatt cataatatga
     8761 ctcagattga ttacttcttc ttacacaagt tccaaaacat tattgatatt gagcatcaat
     8821 taaaagagca tcaaggtgat ttagaatatc ttaaatatgc aaaagattat ggatttagtg
     8881 ataaaacaat agcgcatcgc tttaatatga cggaagaaga agtatatcaa ttgcgtatgg
     8941 aaaatgatat taaacctgtt tacaagatgg ttgatacttg cgcagctgaa tttgaatctt
     9001 caacaccata ttattatggt acatacgaaa ctgaaaatga atccatagtt actgacaaag
     9061 aaaaaatctt agtattaggc tctggaccaa ttcgaatcgg ccaaggtgta gaatttgact
     9121 atgcgacagt tcacgccgtt tgggcaattc aaaaagcagg gtacgaagcg ataattgtga
     9181 ataacaatcc agaaacagtt tcaacagact tctcaatttc tgacaaatta tactttgaac
     9241 ctttaactga agaagatgtg atgaatatca ttaatttaga aaaacctaaa ggtgtcgttg
     9301 tacaatttgg aggacaaaca gcgattaatt tagcagacaa attggctaaa catggtgtta
     9361 aaatacttgg tacttcacta gaaaatctaa atcgtgctga agatagaaaa gaatttgaag
     9421 cactattaag aaaaattaac gtgccacagc cacaagggaa aacagctaca tcacctgagg
     9481 aagcattagc gaatgctgca gaaatcggat atccggttgt agtaagacct tcttatgtat
     9541 taggtggtcg cgcaatggaa attgtagaca atgacaaaga gttagaaaac tatatgaccc
     9601 aggctgtaaa agcgagtccg gaacatccgg tactagtcga tagatattta actggtaaag
     9661 aaattgaagt tgatgcgatt tgtgatggag aaacggtcat tattccagga atcatggaac
     9721 atattgaacg tgctggtgtg catagtggtg actcaatcgc tgtatatcca ccacaaactt
     9781 tgacagaaga cgagttagca acacttgagg actatactat aaaattagct aaaggtttaa
     9841 acatcattgg cttaatcaac attcaattcg ttatagctca cgatggtgtg tatgttttag
     9901 aagtaaatcc acgttctagt agaacggtac cattcttaag taaaattact gatattccaa
     9961 tggcacaatt agctatgcga gcaatcattg gggaaaaact aacagatatg ggttatcaag
    10021 aaggggttca accatatgct gagggtgtct ttgtgaaagc accagtattt agttttaata
    10081 aattgaaaaa tgttgatatt actttaggac ctgaaatgaa gtcaacaggt gaagtgatgg
    10141 ggaaagatac tacattagaa aaggcgttat tcaaagggtt aacaggtagt ggcgttgaag
    10201 ttaaagatca cggtacagta ttaatgaccg tcagtgacaa agataaagag gaagttgtta
    10261 aattggcaca acgcttaaat gaagttggct ataaaatttt agcaacgtct ggaacagcta
    10321 ataaattagc tgagtatgac atacctgcag aagtagtagg caaaattggt ggcgaaaatg
    10381 atttattaac acgtattcaa aatggtgatg ttcaaatcgt tataaataca atgactaaag
    10441 gtaaagaagt agaaagggat ggcttccaaa ttagacgtac tacagttgaa aatggtattc
    10501 catgtttgac atctttagat acagctaatg ccttaacgaa tgtaattgaa agtatgacat
    10561 ttacaatgcg tcaaatgtaa atcaatcaaa ctgtatcggt ggggctgtaa ttaaccattt
    10621 acttaaagaa gtttatatta cagcctcatt attttaatga atttcttaat ataaagggag
    10681 acatatatga tgaaagattt accaattatt gcattagatt ttgaatcaaa agaaaaagta
    10741 aatcaatttt tagatttatt tgatgaatca ttattcgtaa aagtaggtat ggaacttttt
    10801 tatcaagaag gtcctcaatt aattaatgag ataaaagaaa gaggccatga tgtattttta
    10861 gatttaaaac tgcatgatat tcctaataca gttggtaagg cgatggaagg actagctaaa
    10921 ttgaatgttg atctggtaaa tgttcatgct gctggtggcg taaaaatgat gtctgaggcc
    10981 attaaaggat taagaaaaca taatcaagat acaaaaatta ttgcagtaac acagcttacg
    11041 tcaacaacag aagacatgtt acgacacgaa caaaatatac aaacatcgat tgaagaggcc
    11101 gttttaaatt atgccaagtt agcaaatgca gctggtttag atggcgttgt ttgttcacct
    11161 cttgaaagtc gtatgttgac tgaaaagtta ggtacatcat ttttaaaagt aacaccaggt
    11221 attagaccta aaggtgcatc tcaaaatgac caacaccgta ttacgacacc ggaagaagca
    11281 agacagcttg gttcgacgca tattgtagtc ggtagaccga ttacacaaag tgacaatcca
    11341 gtcgaaagtt atcataaaat taaagaaagt tggttagtat aatggctaaa gaaattgcaa
    11401 aatcattatt agatattgaa gctgtaacat tatcaccaaa tgatttatat acatggagtt
    11461 caggtattaa atcaccgatt tactgtgata accgtgttac gttaggttat cctttagttc
    11521 gaggcgcaat ccgcgatggt ttaattaact taattaaaga acactttcct gaagtagaag
    11581 ttatttctgg tactgcaaca gctggtattc cacatgcagc ttttattgct gaaaaattaa
    11641 aattaccaat gaattatgtt cgttcatcaa ataagagtca tggtaagcaa aatcaaatcg
    11701 aaggtgctaa aagtgaaggt aaaaaagtag ttgtgataga agatttaatt tcgacagggg
    11761 gatcttcagt cacagcagtt gaagccttaa aactagcagg tgcagaagta ttaggtgttg
    11821 tagctatctt tacttacggt ttgaaaaaag cagatgatac atttagcaat attcaactac
    11881 ctttttacac tttaagtgat tacaatgaat taattgaagt agctgaaaat gaaggtaaaa
    11941 tttctagtga agatatccaa acattagttg aatggagaga caacttagca taatatagac
    12001 actagaagga ggaattcaac aaatgaatga caaaacatct aatgatttat atgggaagat
    12061 aaaacattgt aacgaattta tcaatcattc aaatgattcc aatctatcta gtagtcacga
    12121 tgtcgacgaa agttcaacga agcaaaaaca tataaaaaat aaaacaacta tagatcataa
    12181 tgatgattta tttaaacatg taaaggatat attacgtaaa caagaacaaa tttaaaaaca
    12241 aaaaagctat agtgtttaat atcaggggct gcttaaccgc atccgaatat tacaaacact
    12301 atagctttta ttatataaaa aatttattag cggataatta actttattaa aatccaacca
    12361 attaagaata cgagtaatag gactaatact ggaatcacat aatgtaacat aacgtccctc
    12421 ctttaactta attttaattg taatcaaatt tgacaataag tcaaaacatt aatacctatg
    12481 ataagtatca tttattaaca tatgtatcat atttttaatc ttgcgtaatt tttatcgtta
    12541 actatgtgat ttaatcaaca ggccaataag acgtttgtca ttcgtgcaca taacggtacg
    12601 acgcgctttt gtcattttaa ttatgttaag ataatagtag ttatagaagt tcaatctata
    12661 ggaggcatag catggatatt ccaaaaatca cgacattttt aatgtttaat aaccaagctg
    12721 aagaagctgt taaactatac acaagcttat ttgaagatag tgagattata acaatggcta
    12781 agtatggtga aaatggacct ggtgatcccg ggactgtaca acactcaata tttacattaa
    12841 atggacaagt attcatggcg attgatgcta atagtggcac agaattacca atgaatcctg
    12901 cgatttcatt atttgttaca gtaaaagata ctattgaaat ggaacgacta tttaatggat
    12961 taaaagatga aggtgccatt ttaatgccaa aaacgaatat gccaccatac agagagtttg
    13021 cttgggttca agataagttt ggagtaagtt ttcaattagc attacctgag taaaaggatt
    13081 cgcacagcgt tgaatgataa agaaacactt tttcttatgc atgcgtttta cctatgtatt
    13141 tctattttaa gtacacatta gcattttagt ttcactcatt tttaaaatca caaagataat
    13201 ttgtaactta aatgatgata cgttatttaa aaacacgata cttcgtttca atgaacgcat
    13261 taaataataa ataaacacct caccataaga aaggactact ttcttttgtg aggtgtttat
    13321 ttgtcgacaa cttgattatg attgtttcat tttttgaata agttcataat caggtgtagc
    13381 atacaaagtt ttttgattgt catatgttac aaacccaggc tttgcacctg atggtttatg
    13441 cacattttta attaatgtat aatcaacagg tatttgtcca gaattaccag cttttgaaaa
    13501 gtatcctgct aacatagccg cttccttgat tgtcgtatca cttggtgcat cattaaatat
    13561 aacgacatgt gaaccaggaa tatcttttgt gtgtaaccat gtgtgagttt ttttagcttt
    13621 tttatttgtt aaataatcat tttgcttgtt attcttacca acatatatat cgtcgccatc
    13681 agttgataca taatgttgta attgaatctg cgctttcttt ttcttagttt gatttttacg
    13741 ctgtttcata aagccttgtt ctgctaattc atctctaatt tcatcaatgt catggacaga
    13801 aatatgatgt aattgttgtt cgattgttga aaaataatct atattgtctt tcgtcaattg
    13861 aatttgatgt tgtaattcac gttctctcgt tttcatacga ttatattgtt tataataata
    13921 ttgagcattt gctgatgggg attttgtagg atttaaagga atgacaactt cttcattcgt
    13981 ataataattc aatgccgtca cttctttatc gccttgctta attcgatata tattagcagt
    14041 gatcaattca ccatataact gttcagtatc tttattttta gactgctcat attcttcaat
    14101 caacttcgct aatttatttt gatatttgta caactgctgt tgaacaaatc gaactaaatc
    14161 attcgcacgt tgtttaacgc gttcacgttc gccacgcgca tcataaaaac gatcaagcaa
    14221 atcatttaat gaatcgtatg taactgtatc atcattaaat tgatttaact ttataaaata
    14281 gaaatcctct ttacctgttt catgattttt atgaaaaata ggagtaggtg gtaacttggt
    14341 ttctgccatt acttcgtcaa atgcttctgg taatgttgat gaagtaataa attgacgacg
    14401 actaacgatt tcattcgtaa ttaaagggct aaatccttca aactgattca ataattgttt
    14461 agcaatatta cctgcgttaa aatcgatata tttcaacacc tctgcacctg taatatcata
    14521 cggatttatt ttgtgctgag taggtggtgc ttcataatta aatcctggca ttactgtacg
    14581 atagtgattc gtatttggtg ttaagtgttt aaatccttca attattttgc gattttcatc
    14641 tactaaaatt aagttactat gtttacccat aatctcaagg atgacagtgc ggtaaatagt
    14701 atcgccaatt tcatctttac tctttatatc gatttcaatg cgacgatcat taccaatttg
    14761 cttaatcgat tcgataatac caccttctaa gtgttttcta aaaacacgcg caaacatggg
    14821 tggattaaat ggattatcat attttttagt agtcaattgt aatcttgaaa agtttggatg
    14881 gattgacaat aacaattgat ggttttgtct attttgacgt acaaccatta gtatcgtgtc
    14941 attatcaggt tgattgattt tgtgaacgcg tcctgttgtt aaaaattgta gagactcaac
    15001 cattttcttt gtaaataagc catcataagc cataatttat cttccacctt cttcatatca
    15061 ttcgtttcat tttaacatga ttgtaacttt tcagtatagt tattgaacca tacattatgc
    15121 catacatttt acattatcta aaagtttgca attcaattta tttaattaaa attaatcgtg
    15181 caatataacg atattgttag acttaataga aattatggca tgcaatttca aatatgctat
    15241 acaatataaa gaacaatgtg atatcatatt taaataatag aagattagct tagagaggtc
    15301 gtaaggcatg gataatgaaa aaggattgtt aatcgtttta tcaggaccat ctggagtagg
    15361 taaaggtact gttagaaaac gaatatttga agatccaagt acatcatata agtattctat
    15421 ttcaatgaca acacgtcaaa tgcgtgaagg tgaagttgat ggcgtagatt acttttttaa
    15481 aactagggat gcgtttgaag ctttaatcaa agatgaccaa tttatagaat atgctgaata
    15541 tgtaggcaac tattatggta caccagttca atatgttaaa gatacaatgg acgaaggtca
    15601 tgatgtattt ttagaaattg aagtagaagg tgcaaagcaa gttagaaaga aatttccaga
    15661 tgcgctattt attttcttag cacctccaag tttagaacac ttgagagagc gattagtagg
    15721 tagaggaaca gaatctgatg agaaaataca aagtcgtatt aacgaagcgc gtaaagaagt
    15781 tgaaatgatg aatttatacg attacgttgt agttaatgat gaagtagaac ttgcgaagaa
    15841 tagaattcaa tgtattgtag aagctgagca cttaaaaaga gagcgcgtag aagctaagta
    15901 tagaaaaatg attttggagg ctaaaaaata atgttaaatc cacctttaaa ccaattaacg
    15961 tcacaaatta aatcaaagta tttaattgca acaactgcag cgaaaagagc gcgtgaaatt
    16021 gatgaacaac ctgaaactga attattaagt gaatatcatt catttaaacc agttggtaga
    16081 gcgttagaag aaattgctga cggtaaaatt cgccctgtta tttcaagtga ttattatggt
    16141 aaagaatagt ttcgaccatt aaaaatatgt gaagctagac gtacatcaaa tacgtcaagg
    16201 catatccaaa tcaaacgcac ctatgtaggt gtgttttttt agtttatcca atgattgatg
    16261 ttataataat actaaatttg tatctataaa aaagtaatga gcatttgtgc gcatatgatg
    16321 atgtaaagcg taaatgtcat agtgatattt aagggagatt gaatatgaag aaaatattat
    16381 tagccgttac aggtggcatt gcggcatata aagcaattga tttgacaagt aagttaacac
    16441 aatctgggta tgaagttcgc gttatgttaa cgaatcacgc acaaaaattt gtgacaccat
    16501 tagcatttca agcaataagt cgaaatgctg tttatacaga tacttttata gaagaaaatc
    16561 cttcagaaat acagcatatt gcattaggtg attgggcaga tgcaatcatt gttgcacctg
    16621 caacggcaaa tacaattgca aaattgagtg taggtattgc tgatgatttg gtgacatcaa
    16681 cgttgctagc aacagagaca ccgaaattta ttgcgcctgc tatgaatgtg catatgtatg
    16741 aaaataaacg tacgcagcaa aatattaata ttttaaaaga agatgggtat cattttatcg
    16801 aaccaggaag cggatttcta gcatgtggtt atgttgctaa aggacgtatg gaagaaccgc
    16861 ttcaaatcgt ttctgtaatt gatgctcatt ttcaaaatag taatcgttta gctaatagtt
    16921 catttcaaga taaacgcgca ttggttacag caggaccaac tattgaagtt atcgatccag
    16981 tcagatttgt atccaatcgt tcttctggaa aaatgggcta tgcaatagct gaagcattgc
    17041 gaaatcgagg agctatcgtg acgttagttg ctggtcctac aacactagag gatccaaaag
    17101 atattgaagt tattcatgtt caaagtgctg aagaaatgtt tgaacaagtg acaagccgat
    17161 ttgacgaaca agatattgtt gtaaaagcag cagccgtatc tgactataca ccggttgatg
    17221 tattagaaca taagatgaaa aagcaggatg gtgatttgtc agtatctttt aaacgtacta
    17281 aagacattct taaatattta ggtgaacata aaacatcaca gtatttaata ggctttgcag
    17341 cagagactga agatattgaa aattatgcac aacaaaaatt acgcaagaaa aatgcagatg
    17401 tgattatttc aaataatgtt ggggatatgt ctatcggatt tagttctgat gataatgaat
    17461 tgacaatgca ttttaaaaat aatgaaaaag taaatatcaa gaaaggaaaa aaagtagtat
    17521 tagctgcaca aattttagat gaactagaaa ctaggtggca ataatgatag cgaaagtcat
    17581 agtcgatgtc gcgtcgaaga gcgttgacta taaatttgat tatataattc ccgaacaact
    17641 cgaatctgtc atccaacctg gtgtgcgtgt aattgtacct tttggaccaa gaacgattca
    17701 aggttatgta atggaagtaa cagcagaacc tgatgcacaa cttgacgttt cgaagttaaa
    17761 aaaaatcata gaagtgaaag atatacaacc agaattaaca tcagaattaa tagctttaag
    17821 tgagtggatg ggttcaactc atgtcattaa acgtatttct atgctagaag tgatgcttcc
    17881 gagtgctatt aaagcgaagt ataaaaaagc atttaagatg aaagatgaca tagagctacc
    17941 ttcagcttta ttacaaaaat ttgataagca tggttactat tattataaag atgcgcaaaa
    18001 aaataatgat attcaattgc ttatgaagtt gttaaaagat gatatcgttg aagaaaaaac
    18061 gattctcaca caaaatataa ctaaaaaaac caagcgtgct gttcgtgtca ttgaagggta
    18121 tcatcctgat gaagtattag ctaagttgga gaaagttatt aaacaatacg atttgtatgc
    18181 ttacttgtct gaagaacaac ataaaacaat atttttaact gatattgagg atatgggctt
    18241 ttcaaaatcc agtttagatg gacttatcaa aaaaggttat gttgaaaaat atgacgcggt
    18301 tgttgaaaga gacccattta aagatcgtgt tttcgaacaa gaatcaaaac agcaattaac
    18361 agaagaccaa tataaagcat atgaagcgat taaagctaaa attgtaagcc aagagcaaga
    18421 aacattttta cttcatggtg tgacgggatc aggtaaaaca gaagtatatt tacaaacgat
    18481 agaagatgtt ttaagccaag gaaaacaggc gatgatgtta gttcctgaaa tcgctctaac
    18541 accgcaaatg gttttacgct tcaaacgtcg atttggtgat gacgttgctg tattacattc
    18601 tggcttatct aatggggaac gttatgatga atggcaaaaa attagggatg gtcgtgcgag
    18661 agtaagtgtt ggtgc
//
LOCUS       DALAGD010000062        18646 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.063, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000062 DALAGD010000000
VERSION     DALAGD010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18646
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.063"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            46..1152
                     /gene="rpoD"
                     /locus_tag="O2701_001735"
     CDS             46..1152
                     /gene="rpoD"
                     /locus_tag="O2701_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCZ4293623.1"
                     /translation="MSDNTVKIKKQTIDPTLTLEDVKKQLIEKGKKEGHLSHEEIAEK
                     LQNFDIDSDQMDDFFDQLNDNDISLVNEKDSSDTDEKLNPSDLSAPPGVKINDPVRMY
                     LKEIGRVNLLSAQEEIELAKRIEQGDEVAKSRLAEANLRLVVSIAKRYVGRGMLFLDL
                     IQEGNMGLIKAVEKFDFNKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LIRVQRQLLQDLGRDPAPEEIGEEMDLPAEKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDQEAQSPSDHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGK
                     VFGVTRERIRQIEAKALRKLRHPSRSKRLKDFMD"
     gene            1283..1960
                     /locus_tag="O2701_001736"
     CDS             1283..1960
                     /locus_tag="O2701_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HCZ4293624.1"
                     /translation="MISLNNRLTTVSRFLKQGTIADIGSDHAYLPIYAIQNHLCECGI
                     AGEVIQGPFQAAVKNVAANQLVDRIDVRLGDGLSVIQPEDVIDNITICGMGGPLIAKI
                     LKDGQDKLSQHPRLILQSNIQTENLRQTLQQLNYEIIDEIIMEEKGHIYEIVVAEYST
                     ELIELSSDELKFGPKLLNNKNEYFIKKWQRELEALYHIKSKLNTEQHHQRLAQINDEI
                     AVIERVL"
     gene            1963..3063
                     /locus_tag="O2701_001737"
     CDS             1963..3063
                     /locus_tag="O2701_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCZ4293625.1"
                     /translation="MKIADLMTLLDHHVPFSTAESWDNVGLLIGDEDVEVTGVLTALD
                     CTLEVVNEAIEKGYNTIISHHPLIFKGVTSLKANGYGLIIRKLIQHDINLIAMHTNLD
                     VNPYGVNMMLAKAMGLKNISIINNQQDVYYKVQTYIPKDNVGPFKDKLSENGLAQEGN
                     YEYCFFESEGRGQFKPVGEANPTIGQIDKIEYVDEVKIEFMIDAYQKSRAEQLIKQYH
                     PYETPVFDFIEIKQTSLYGLGVMAEVDNQMTLEDFAADIKSKLNIPSVRFVGESNQKI
                     KRIAIIGGSGIGYEYQAVQQGADVFVTGDIKHHDALDAKIHGVNLIDINHYSEYVMKE
                     GLKALLMNWFNTEKINLDVEASTINTDPFQYI"
     gene            3177..4523
                     /locus_tag="O2701_001738"
     CDS             3177..4523
                     /locus_tag="O2701_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCZ4293626.1"
                     /translation="MAKHPFEQFNLESSLIDAVKDLNFEKPTEIQNRIIPRILKRTNL
                     IGQSQTGTGKSHAFLLPLMQLIDSEIKEPQAIVVAPTRELAQQLYDAANHLSQFKAGV
                     SVKVFIGGTDIEKDRQRCNAQPQLIIGTPTRINDLAKTGHLHVHLASYLVIDEADLMI
                     DLGLIEDVDYIAARLEDNANIAVFSATIPQQLQPFLNKYLSHPEYVAVDSKKQNKKNI
                     EFYLIPTKGAAKVEKTLNLIDILNPYLCIIFCNSRDNANDLARSLNEAGIKVGMIHGG
                     LTPRERKQQMKRIRNLEFQYVIASDLASRGIDIEGVSHVINFDVPNDIDFFTHRVGRT
                     GRGNYKGVAITLYSPDEEHNISLIEDRGFVFNTVDIKDGELKEVKAHNQRQARMRKDD
                     HLTNQVKNKVRSKIKNKVKPGYKKKFKQEVEKMKRQERKQFSKQQNRQKRKQNKKG"
     gene            4533..5423
                     /locus_tag="O2701_001739"
     CDS             4533..5423
                     /locus_tag="O2701_001739"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCZ4293627.1"
                     /translation="MLLGSHVSMSGKKMLEGSAIEAHEYGETTFMIYTGAPQNTRRKS
                     IEDLNITKGHEVMEKYGLSNIVVHAPYIINIANTTKPETFNLGVDFLQQEIERTQAIG
                     AKDIVLHPGAHVGAGVDAGINKIIEGLNEVLTNDNNVRIALETMAGKGTEIGRSFEEL
                     ARIIDGVHNNERLSVCFDTCHTHDAGYNVKEDFDGVLNEFDKIIGVDRIKVVHVNDSK
                     NDRGAQKDRHENIGFGYIGFDALNYIVHHDSFKDIPKILETPYVGEDKKNKKPPYKLE
                     IEMLKQQQFDPELKNKVMQQ"
     gene            5549..6334
                     /locus_tag="O2701_001740"
     CDS             5549..6334
                     /locus_tag="O2701_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCZ4293628.1"
                     /translation="MRGENTMTPVFELKNVNYYYDHKKVLENINIKINKGEFLAIVGP
                     NGAGKSTLLKLILGLLPLQSGEIFVEGIDFKNKKTSIKLSYVSQKANAFNSGFPASVK
                     EVVLSGLTKTKRLFQTFNSKDNEKVIKVLERLNISDLIHKNIAELSGGQQQRVMIARA
                     LISEPAVLVLDEPTNGIDAKHVSEFYNTLDQLKQEGITIILVTHDIGVVADTATEVAC
                     LNKHLHFHGTTDEFKSLDEVEISKIYGHPVRFVDHQHNRECCN"
     gene            6376..7239
                     /locus_tag="O2701_001741"
     CDS             6376..7239
                     /locus_tag="O2701_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCZ4293629.1"
                     /translation="MIDALLNFDFMRYSLISGILIGFIAPLIGAFIVVRRLSLIADAL
                     SHVTLGGISFGMFLLTIMPTLVFINPMWFGILFAIVGALLIEKLRTSYTAYQEIAIPI
                     IMSAGIALSAIFISLADGFNQEIVGLLFGSISAVNISDLTTIIVIAIIVVLFITLFYK
                     ELFILSFDEEYSKVIGIPKWIQFLFIVIVAMVISASMRVVGILLVSALITLPIAISMR
                     ITKSFKQLILLSVFLGELSVILGLVLAFYMDISPGGVIVVLLVILLMITMAYQKMRMK
                     FKKGANINEYK"
     gene            7226..7636
                     /locus_tag="O2701_001742"
     CDS             7226..7636
                     /locus_tag="O2701_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCZ4293630.1"
                     /translation="MNTNDAIKILKENGLKYTDKRKDMLDIFVEEDKYINAKYIQQVM
                     DENYPGISFDTIYRNLHLFKDLGIIENTELDGEMKFRIACTNHHHHHFICEKCGDTKV
                     IDYCPIDQIKLSLPGVNIHKHKLEVYGVCESCQD"
     gene            7913..8512
                     /locus_tag="O2701_001743"
     CDS             7913..8512
                     /locus_tag="O2701_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCZ4293631.1"
                     /translation="MAFELPKLPYAFDALEPHFDKETMEIHHDRHHNTYVTKLNAAVE
                     GTDLESKSIEEIVANLDSVPANIQTAVRNNGGGHLNHSLFWELLSPNSEEKGTVVEKI
                     KEQWGSLEEFKKEFADKAAARFGSGWAWLVVNNGQLEIVTTPNQDNPLTEGKTPILGL
                     DVWEHAYYLKYQNKRPDYIGAFWNVVNWEKVDELYNATK"
     gene            8633..10708
                     /locus_tag="O2701_001744"
     CDS             8633..10708
                     /locus_tag="O2701_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HCZ4293632.1"
                     /translation="MLKRLKEKSNDEIVQNTINKRINFIFGVIIFIFAVLVLRLGYLQ
                     IAQGSHYKQIIKNDENITVNESVPRGRILDRNGKVLVDNASKMAITYTRGRKTTQSEM
                     LDTAEKLSKLIKMDTKKITERDKKDFWIQLHPKKAKAMMTKEQAMLADGSIKQDQYDK
                     QLLSKIGKSQLDELSSKDLQVLAIFREMNAGTVLDPQMIKNEDVSEKEYAAVSQQLSK
                     LPGVNTSMDWDRKYPYGDTLRGIFGDVSTPAEGIPKELTEHYLSKGYSRNDRVGKSYL
                     EYQYEDVLRGKKKEMKYTTDKSGKVTSSEVLNPGARGQDLKLTIDIDLQKEVEALLDK
                     QIKKLRSQGAKDMDNAMMVVQNPKNGDILALAGKQINKSGKMTDYDIGTFTSQFAVGS
                     SVKGGTLLAGYQNKAIKVGETMVDEPLHFQGGLTKRSYFNKNGHVTINDKQALMHSSN
                     VYMFKTALKLAGDPYYSGMALPSDISSPAQKLRRGLNQVGLGVKTGIDLPNETRGQIE
                     PLTNNPGNYLDLSIGQYDTYTPLQLSQYVSTIANDGYRIQPHIGLTIHESTNKDEVGP
                     LKKKINGTVLNKVNNTEKEIKQIQEGFKMAFNDKDGTGYVSFKDTVVPTAGKTGTAEV
                     FQNGEPRVNSTYIGYAPIDDPKLAFSIVYTNQPVPPPWLTGGDLGRDVINYYFKQLGK
                     NDKNKDKDK"
     gene            10821..10970
                     /gene="rpmG"
                     /locus_tag="O2701_001745"
     CDS             10821..10970
                     /gene="rpmG"
                     /locus_tag="O2701_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCZ4293633.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERVEMKKFCSRENKQTL
                     HRETK"
     gene            11179..11718
                     /locus_tag="O2701_001746"
     CDS             11179..11718
                     /locus_tag="O2701_001746"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCZ4293634.1"
                     /translation="MTKNEIRKYILHKMKNFNKAEKRKADTWLRNQFFATEEYKEANA
                     IALVLSFNHEVDTFSIIEQALMDHKRIFVPKMDYLNHQMTFKEIFNLKDIDVDNKGIY
                     YPTSKGETTNNLDLIVVPGVGFQDDGYRIGYGGGYYDRFLANYQTKTISLLYDFQITS
                     FEPESFDQPVDKLIIYQAA"
     gene            11730..13193
                     /locus_tag="O2701_001747"
     CDS             11730..13193
                     /locus_tag="O2701_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCZ4293635.1"
                     /translation="MNIDKQFWKTIYYWIRYLNFDIVSREKDDQEIWLAHKRKKQVVI
                     FKQHIKSTQEIRFDKAKVLEHKDEIANFISFEPQSFEFYYFTESEFSEEQLNEVSPIR
                     IKFNVIRHTKDLIKHMPNIFLARLISEDNDKKTYMFYKRKVLTDNFLDKYMQKFSPAT
                     YTIIFVNVLIWLCMILYLNNFSDVKLLDVGGLVHFNVVHGEWYRIVTSIFLHFSFEHI
                     LMNMLSLFIFGKIVEAIIGSWRMLTVYFIAGLFGNFVSLSFNTTTISVGASGAIFGLI
                     GSIFAMMYVSKTFNKKMLGQLLIALVILVGVSLFMSNINIVAHIGGFIGGLLITLIGY
                     YYKVNRNIFWILLIGMLVIFIALQIRIFTIKEDNIYNKLIKDDMTSGNYDNAQNIVKQ
                     TINKNYADDQTYYLSGMIMATINSKSEGMTEWERGLRMFPKSGLLNFELAIANRSLND
                     DEKALKYVRKALNADPKNTDYINLEKELTKSNESKNK"
     gene            13174..13377
                     /locus_tag="O2701_001748"
     CDS             13174..13377
                     /locus_tag="O2701_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HCZ4293636.1"
                     /translation="MSRKINNFYDVQQLLKSYGFLIYFKNPEDMYEMIQQEISSLYQY
                     ELLSKEEYLKCTLIINQRRNEQK"
     gene            13374..14360
                     /locus_tag="O2701_001749"
     CDS             13374..14360
                     /locus_tag="O2701_001749"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HCZ4293637.1"
                     /translation="MTKIILAADVGGTTCKLGIFTPELEQLHKWSIHTDTSDSTGYTL
                     LKGIYDSFVEKVNENNYNFSNVLGVGIGVPGPVDFEKGTVNGAVNLYWPEKVNVREIF
                     EQFVDCPVYVDNDANIAALGEKHKGAGEGADDVVAITLGTGLGGGIISNGEIVHGHNG
                     SGAEIGHFRADFDQRFKCNCGRSGCIETVASATGVVNLVNFYYPKLTFRSSILELIKE
                     NKVTAKAVFDAAKAGDQFCIFITEKVANYIGYLCSIISVTSNPKYIVLGGGMSTAGPI
                     LIENIKTEYHNLTFAPAQFETEIVQAKLGNDAGITGAAGLIKTYVLDKEGVK"
     gene            14360..14689
                     /locus_tag="O2701_001750"
     CDS             14360..14689
                     /locus_tag="O2701_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HCZ4293638.1"
                     /translation="MAIVDVVVIPVGTEGPSVSKYIADIQKKLQEYKAMGKIDFQLTP
                     MNTLIEGELSDVLEVVQVIHELPFDKGLSRVCTNIRIDDRRDKSRKMNDKLTSVQKHL
                     ENSGENL"
     gene            14686..15309
                     /locus_tag="O2701_001751"
     CDS             14686..15309
                     /locus_tag="O2701_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCZ4293639.1"
                     /translation="MRISSLTLGLVDTNTYFIENDKAVILIDPSGESEKIIKKLNQIN
                     KPLKAILLTHAHFDHIGAVDDIVDRFDVPVYMHEAEFDFLKDPVKNGADKFKQYGLPI
                     ITSKVTPEKLNEGSTEIEGFKFNVLHTPGHSPGSLTYVFDEFAVVGDTLFNNGIGRTD
                     LYKGDYETLVDSIQDKIFELEGDLPLFPGHGPYTTVDDEQLNPFLHG"
     gene            15361..16335
                     /locus_tag="O2701_001752"
     CDS             15361..16335
                     /locus_tag="O2701_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HCZ4293640.1"
                     /translation="MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQ
                     IGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYLFNKIYFLRISTLPLSLGQESCVI
                     RIVPQFFQPQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
                     VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVI
                     QASLSGHLVLTTLHATDCKGAILRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLV
                     CEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTMHKYI"
     gene            16307..17377
                     /locus_tag="O2701_001753"
     CDS             16307..17377
                     /locus_tag="O2701_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HCZ4293641.1"
                     /translation="MKLQCINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSF
                     QFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIGYSDTIVMQVYLAERFGNIIDVL
                     EETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
                     SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNNLNMLNKINFVMKLPLISGYFQ
                     LFKTYFVTNELVLFYKNGITLQSIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQIL
                     EKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIKQTQFLQPILFLILG
                     LFIVAIYLVIMLPMFQMMQSIK"
     gene            17391..17702
                     /locus_tag="O2701_001754"
     CDS             17391..17702
                     /locus_tag="O2701_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCZ4293642.1"
                     /translation="MFKFLKKTQAFTLIEMLLVLLIISLLLILIIPNIAKQTAHIQST
                     GCNAQVKMVNSQIEAYALKHNRNPSSIEDLIADGFIKEAQKTCKSGETITISNGEAVA
                     N"
     gene            17680..18126
                     /locus_tag="O2701_001755"
     CDS             17680..18126
                     /locus_tag="O2701_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HCZ4293643.1"
                     /translation="MEKQLQIRKQSAFTMIEMLVVMMLISIFLLLTMTSKGLSNLRVI
                     DDEANIISFITELNYIKSQAIANQGYINVRFYENSDTIKVIENNNIRFLKLKVGKIIN
                     VAKVDIIAFDKKGNINKFGSITIYNNNSIYRIIFHIEKGRIRYEKL"
     gene            18113..18412
                     /locus_tag="O2701_001756"
     CDS             18113..18412
                     /locus_tag="O2701_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGE"
                     /protein_id="HCZ4293644.1"
                     /translation="MKSYKCKGSFLIDSMAGFLLIGLITLLLIPMMNQMQASINHKLQ
                     TIDASKVILTTVSKINKEELKKGVTIGKYDIKQSDQQICAISKNTTSYQKTCIQY"
     gene            18330..>18646
                     /locus_tag="O2701_001757"
     CDS             18330..>18646
                     /locus_tag="O2701_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCZ4293645.1"
                     /translation="MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVI
                     SITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDILDDFKGVDRNDIEIRQHRIILH
                     KGE"
BASE COUNT         6961 a         2421 c         3366 g         5898 t
ORIGIN      
        1 tgaataaatt tcgaatgttc atatttaaaa tcgggaggcc gtttcatgtc tgataacaca
       61 gttaaaatta aaaaacaaac aattgatccg acattaacat tagaagatgt taagaagcaa
      121 ttaattgaaa aaggtaaaaa agagggtcat ttaagtcatg aagaaattgc tgaaaaactt
      181 cagaattttg atatcgactc tgatcaaatg gatgatttct ttgatcaatt aaatgataat
      241 gatatttcac tagttaatga aaaagatagt tcagatactg acgagaaact gaatccaagt
      301 gatcttagtg cccctccagg tgttaaaata aatgacccag ttcgtatgta ccttaaagaa
      361 attgggcgtg ttaacttatt aagtgcacaa gaagaaatcg aattagccaa acgtattgaa
      421 caaggtgatg aagtagcaaa atcaagactt gcagaagcga acttacgttt agttgtaagt
      481 attgctaaaa gatacgtagg tcgtggtatg ttattccttg atttaatcca agaaggtaat
      541 atgggtctta ttaaagctgt tgaaaaattt gactttaaca aaggatttaa gttttcaaca
      601 tatgcaacat ggtggattag acaagcaatc actcgtgcaa ttgctgacca agcacgtacg
      661 attcgtatcc ctgtgcatat ggtagaaaca attaataaat taattcgtgt tcaacgtcaa
      721 ttattacagg acttaggtcg agatccagca ccagaagaaa ttggtgaaga aatggattta
      781 ccagcagaaa aagttcgtga aattttaaaa attgcgcaag aacctgtttc attagaaaca
      841 ccaattggtg aagaagatga tagtcattta ggagacttta ttgaggatca ggaagcacaa
      901 agtccttcag atcatgctgc ttatgaatta ttaaaagagc aattagaaga tgtgcttgat
      961 acattaactg atagagaaga aaatgtatta cgattaagat ttggtcttga tgacggcaga
     1021 acaagaacac ttgaagaagt tggtaaagtt ttcggtgtta cacgtgaacg tattcgacaa
     1081 attgaagcaa aagcacttag aaaattaaga catccaagtc gtagtaaacg tttgaaagac
     1141 tttatggatt aatttatatc aattttaatg attgatacca agacatgaat aagcgcttat
     1201 ttatttttag attaagattt aacttgagtg aggttggtac attgaataat gttcaacctc
     1261 ttttttcata aaggagaata aaatgatttc gttaaataac cgattaacga cggtaagtcg
     1321 atttttaaaa caaggtacaa tcgcggatat tggctcagat catgcttatt tgccaatcta
     1381 tgcaattcaa aaccatttat gcgaatgcgg tattgctgga gaagttattc aaggcccttt
     1441 tcaagctgct gtgaaaaatg tagctgcaaa ccaattagta gatagaattg atgtacgttt
     1501 aggggatggc ttaagtgtta ttcaacctga ggacgtaatc gataatatca caatttgtgg
     1561 tatgggcgga ccacttattg ctaaaatttt aaaagatggg caagataagt taagtcaaca
     1621 tccaaggttg attttacaaa gtaatatcca aactgaaaat ttaagacaaa cattacaaca
     1681 attgaattat gaaataattg atgaaataat aatggaagaa aaaggccata tttatgaaat
     1741 tgttgtagca gaatattcta cagaattaat agagctatca tctgatgaat tgaaattcgg
     1801 accaaaattg ctgaacaaca aaaatgaata ttttataaaa aaatggcaaa gagagttaga
     1861 agcactttat catattaaat cgaaactcaa tactgaacaa catcatcaac gtttagctca
     1921 aattaatgac gaaattgctg taattgaaag ggtgttatag taatgaaaat agctgattta
     1981 atgacattgt tagatcatca tgtgccattt agtactgctg aatcttggga taatgtagga
     2041 ttgttaatag gtgatgaaga tgttgaagtt actggtgttt taacagcatt agactgtacg
     2101 ttggaagtag taaatgaagc aatcgaaaaa ggttataata ctattattag tcatcatcct
     2161 ctaatcttta aaggcgtaac atcattaaaa gctaatggtt atggtttgat cattagaaaa
     2221 ctaattcaac atgacattaa tttaatagcg atgcatacaa atttagatgt aaatccgtat
     2281 ggtgtcaata tgatgttggc gaaggcgatg ggtttgaaga acatttcaat aataaataat
     2341 caacaagatg tatactataa agttcaaaca tatataccta aggataatgt tggaccattt
     2401 aaagataagc ttagtgaaaa tggattagcg caagaaggta attatgaata ttgtttcttt
     2461 gaaagtgaag gaagagggca attcaaacca gttggtgaag ctaatccaac aataggacaa
     2521 attgataaaa ttgaatatgt agatgaagtt aaaattgaat ttatgataga tgcatatcaa
     2581 aagtcaaggg ctgagcaatt aattaaacaa taccatccat atgaaacacc ggtatttgat
     2641 tttattgaga taaaacaaac atccctttat ggacttggcg ttatggcaga agtggataat
     2701 caaatgacat tggaagattt cgcagctgat attaaatcta aattaaatat cccaagtgtc
     2761 cgttttgttg gtgagtctaa tcagaaaatt aaacgtattg caattattgg tggttcaggt
     2821 attggatatg aatatcaagc tgtccaacaa ggcgcagatg tctttgttac aggtgatatt
     2881 aaacatcatg atgccttaga tgctaaaatt catggtgtga atttaattga tattaatcat
     2941 tacagcgaat atgtgatgaa agaaggttta aaagcgttac taatgaattg gttcaataca
     3001 gaaaaaataa atttagatgt tgaagcatct acaattaata cagatccatt tcaatatatt
     3061 taaactaaaa tctcgggatt tctaaatttt gaaatttcga ggttttaata tttttattta
     3121 aaatagcaca tttatacttt ataatagtaa agatgaacat ataaggaggc caaatcatgg
     3181 caaaacatcc attcgaacaa tttaatctag aatctagttt aattgacgct gtgaaagacc
     3241 ttaattttga aaaaccaact gaaattcaga atcgaattat tccaagaata ctaaagagaa
     3301 caaatttaat tggtcaatct caaacgggta cagggaaatc tcatgcattt ttattaccat
     3361 taatgcagtt aattgatagt gaaataaaag aaccacaagc aatcgtagtt gcaccaacaa
     3421 gagaacttgc acaacaacta tacgatgcag cgaaccattt aagccaattt aaagctggtg
     3481 tttcagttaa agtttttatt ggtggtacag atatagagaa agatagacaa cgttgtaatg
     3541 cacaaccaca attgattata ggcaccccta ctagaattaa tgacttagct aaaacgggac
     3601 atttacatgt gcacttagca tcatatttag ttattgatga agcggatctt atgattgact
     3661 taggattaat tgaagatgta gattacattg ctgcaagatt ggaagataat gcaaatattg
     3721 cggtgtttag tgctacaatc ccacaacagt tacaaccatt tttaaataaa tatttaagtc
     3781 atccagaata tgtagctgtc gacagtaaaa aacaaaataa aaagaacatc gaattctatt
     3841 taatacctac taaaggtgca gctaaagttg aaaagacttt aaatttaatt gatatactaa
     3901 atccatactt atgtattatt ttctgtaata gtagagataa tgcaaatgat ttagcacgtt
     3961 cactaaatga agctggtatt aaagttggta tgattcatgg tggcttaacg ccacgtgaac
     4021 gtaaacaaca aatgaaacgt atacgtaatt tagaattcca atacgttatt gccagcgatt
     4081 tagcatctcg tggtattgat attgaaggtg ttagtcatgt catcaatttt gatgtgccaa
     4141 atgatattga cttctttacg catagagtcg gacgaactgg tcgtgggaat tataaaggtg
     4201 tagcaattac gctttatagt cctgatgaag aacacaatat ttcattaata gaagatcgcg
     4261 gttttgtatt caatactgtt gatattaaag atggtgagtt aaaagaagtt aaagcgcaca
     4321 atcagcgtca agcaagaatg cgcaaagatg accatttaac taatcaagtg aagaacaaag
     4381 ttcgaagtaa aattaaaaac aaagttaaac caggttataa gaagaaattt aaacaagaag
     4441 ttgaaaaaat gaaacgtcaa gagcgtaagc aatttagtaa gcagcaaaat agacaaaaac
     4501 gtaagcaaaa caaaaaaggt taggtgtgtt ttatgttatt aggatcacat gtttcaatga
     4561 gtggtaaaaa gatgttagaa ggttctgcta tagaagcgca tgaatatggt gaaacaacat
     4621 ttatgattta tactggtgca cctcaaaaca cgcgccgtaa aagtattgaa gatttaaata
     4681 taactaaagg tcatgaagtg atggaaaaat atggtttatc taatattgtt gttcatgcac
     4741 catacatcat taatattgca aatacaacca aaccagaaac gtttaatctt ggtgtagatt
     4801 tcttgcaaca agaaattgaa agaacgcaag ctataggggc taaagatatt gtattacatc
     4861 caggtgcgca tgttggagct ggtgttgatg ccggaattaa taagattatt gaaggattaa
     4921 atgaagtttt aacgaatgac aataatgtac gtattgcgct tgaaacgatg gcaggtaaag
     4981 gtacagagat tggtcgctca tttgaagagt tagcacgtat tattgatgga gttcataata
     5041 acgaacgttt atcagtatgt tttgatacat gtcatacgca tgatgctgga tataatgtta
     5101 aagaggactt tgatggcgta ttaaatgaat ttgataaaat cattggagtc gacagaatca
     5161 aagttgttca tgtcaatgat tctaaaaatg accgtggcgc tcaaaaagac cgacatgaaa
     5221 atattggctt cggttacatt ggttttgatg cattaaatta cattgtgcat catgatagtt
     5281 ttaaagatat tccgaaaatc ttagaaacac cttatgttgg tgaagataag aaaaataaaa
     5341 aaccgccata taaacttgaa attgaaatgt taaaacaaca gcaatttgac ccagaattaa
     5401 aaaataaggt tatgcaacaa taaaatgata atgctctctt caagaaatgc gataaataaa
     5461 acttgaaggg ggcatatctc tttattttgt ctaattttga gtcgtaaaca ttactgttta
     5521 catatgttac taggagttgt atagttgaat gcgaggtgag aatacgatga caccagtctt
     5581 tgaattgaaa aatgtcaatt actactatga tcataaaaaa gtgttagaaa atataaacat
     5641 taaaataaat aaaggtgaat ttttagcaat tgttggacca aatggtgctg gtaaatcaac
     5701 attattgaag ttgattctag ggttattacc tttacaaagt ggtgagattt ttgttgaagg
     5761 tattgatttt aaaaataaga aaacatcaat taaattaagc tatgtatcac aaaaagcaaa
     5821 tgcctttaat tcaggtttcc cagcaagtgt taaagaagtt gttttaagcg gattaacaaa
     5881 gacaaaacgt cttttccaaa catttaatag caaagataat gaaaaagtga ttaaagtact
     5941 agaaagactg aatataagtg atttaattca taaaaatata gcagaattat caggtggtca
     6001 acaacaacgt gtaatgattg ctcgagcatt gatttcagaa cctgcagtat tagtacttga
     6061 tgaaccaacg aatggtattg atgcaaaaca tgtaagtgaa ttttataata ctttagatca
     6121 attaaaacaa gaaggtatca ccattatctt agttactcat gatatcggtg ttgtagcaga
     6181 tactgctact gaagtagcat gtttaaataa gcatttgcat ttccatggta caactgatga
     6241 gtttaaatca cttgatgaag ttgaaatttc aaaaatttat ggacatcctg tacgttttgt
     6301 cgatcatcag cataatcgag aatgttgtaa ttaataaaga tttgagttta tttaaaatgg
     6361 aaaggcgtgt cacaattgat agatgcatta ttgaattttg attttatgag gtactcttta
     6421 attagtggta tcttgatagg ttttattgcg cctctaatcg gtgcttttat cgttgttaga
     6481 cgactatcac ttatagctga tgctctaagt catgtaactt taggtggtat atctttcggt
     6541 atgtttttac ttactattat gccaacacta gtatttatta atccaatgtg gtttggaatc
     6601 ttattcgcaa tagtaggtgc gcttctaatt gaaaaattaa gaacgtcata cactgcttac
     6661 caagaaattg ctattccaat tataatgagt gctggtatcg ccttgagtgc aatcttcatt
     6721 tcattagctg atgggttcaa tcaagaaatt gttggattgc tatttggctc aataagcgca
     6781 gtgaatataa gtgatttaac aacgattatt gtcattgcca tcatagttgt tctgtttatt
     6841 acattgtttt ataaagaatt atttatattg tcatttgatg aggaatatag taaagtcatt
     6901 ggcattccta aatggattca atttttattt attgttattg tagcgatggt tatttctgcc
     6961 tcaatgcgtg ttgtagggat actgttagta agtgcgttaa tcacgttacc aattgcaata
     7021 agtatgcgaa taacaaaaag ttttaagcaa ttgatattac taagtgtatt tttaggtgaa
     7081 ttatcagtaa tattaggctt agtcctagct ttctatatgg acatttctcc gggtggtgta
     7141 attgtagttc tacttgttat tttacttatg attacaatgg cttatcagaa aatgcgaatg
     7201 aagtttaaaa agggagctaa tatcaatgaa tacaaatgat gctattaaaa ttttaaaaga
     7261 gaacggttta aaatatacag ataaacgtaa agatatgtta gatatttttg tcgaagaaga
     7321 taagtatata aacgcaaagt atatacaaca agttatggat gaaaattatc ctggaatttc
     7381 attcgacaca atatatagaa acctgcactt atttaaagat ttaggaatta ttgaaaatac
     7441 agaacttgat ggtgaaatga agtttagaat cgcttgtaca aaccatcatc atcatcattt
     7501 tatctgtgaa aagtgtggag atacaaaggt aatagattat tgtccaatag atcagataaa
     7561 attatcacta cctggtgtta atattcacaa acacaaactt gaagtttatg gtgtatgtga
     7621 gtcttgccaa gattaatata aagaaatgag atttatgcac atttggtcag atgtatgcat
     7681 aaatctcatt tctttatatt atagaagata aaggttcatt tcatactccg ttcgtatgat
     7741 tttatggcaa atctgttaac tttttatata caaaatgttt aattattttt gatttgagtt
     7801 atattaaatg agtagagaaa ataataagta gtagaattta agcaaattct ttgttgaacc
     7861 agtcactgct tgttattatt tacttacaga cattttagga ggatgattat ttatggcttt
     7921 tgaattacca aaattaccat acgcatttga tgcattagaa ccacattttg acaaagaaac
     7981 tatggaaatt caccatgaca gacatcataa cacgtatgtt acgaaattaa atgctgcagt
     8041 agaaggtaca gatttagaat ctaaatctat tgaagaaatt gttgctaatt tagacagtgt
     8101 accagctaac atccaaactg ctgtacgtaa taatggcggt ggacatttaa accattcatt
     8161 attctgggag ttactttcac caaactcaga agaaaaaggt actgtagtag aaaaaattaa
     8221 agaacaatgg ggttctttag aagaatttaa aaaagaattt gctgacaaag cagctgcacg
     8281 ctttggttca ggttgggctt ggttagtcgt aaacaatggc cagttagaaa ttgtgactac
     8341 accaaaccaa gataatccat taactgaggg taaaacacct attttaggtt tagacgtatg
     8401 ggaacacgct tattacctaa aatatcaaaa caaacgccct gactacattg gcgcattttg
     8461 gaatgtagtt aactgggaaa aagttgacga attatataat gcaacaaaat aaagtatgat
     8521 tacttgttcg gtctcttaaa tgagaccgtt atttttttgt caaaaagata gaaataattt
     8581 ctaaattcat atatgattta aagtgaaaga ctttgaatag aggtaggtag ttttgttaaa
     8641 aagactaaaa gaaaaatcaa atgatgaaat cgttcaaaat acaattaaca agagaattaa
     8701 ctttatattt ggtgtgatta tatttatttt tgcagtacta gtactacgtt taggttattt
     8761 acaaatcgca caaggctcac attataaaca aattataaaa aatgatgaaa acattacagt
     8821 gaatgagtct gtgccaagag gtcgtatttt agacagaaat gggaaagttt tagttgataa
     8881 tgcttctaaa atggctatta catatactag gggtcgaaaa acaacacaat cggaaatgtt
     8941 ggatacggct gaaaagttat caaagctaat caagatggat actaagaaaa ttacagaacg
     9001 tgataagaaa gatttctgga ttcagttgca tcctaaaaaa gcaaaagcaa tgatgacaaa
     9061 agaacaagct atgttagcag atggaagtat taaacaagat caatatgata aacaactgtt
     9121 atcgaaaatc ggaaaatcac aattagatga attgtcttct aaagatttac aagttttagc
     9181 tatttttcga gagatgaatg caggaacagt tttagatcca caaatgataa aaaatgaaga
     9241 tgtcagtgaa aaagagtatg cagcagtttc tcagcaactt tccaaattac caggtgttaa
     9301 cacgtctatg gattgggata gaaaatatcc atatggcgat actttaagag gtatattcgg
     9361 agatgtatcg acacctgctg aaggtattcc aaaagaattg acagaacatt acttatccaa
     9421 aggatattca cgcaatgatc gtgttggaaa atcttaccta gaatatcaat atgaagatgt
     9481 attgcgtggt aagaagaaag aaatgaaata cacaacggac aaatctggta aagttacatc
     9541 ttcagaagtg ttaaatcctg gcgctcgcgg tcaagatttg aaattaacga tcgatataga
     9601 tcttcaaaaa gaagtagaag cattattaga taaacaaatt aagaagcttc gcagtcaagg
     9661 tgccaaagat atggataatg caatgatggt tgtacaaaat cctaaaaatg gagacattct
     9721 tgcgcttgcc ggaaagcaga ttaataagag tggtaaaatg actgattatg acattggtac
     9781 gtttacttct caatttgcgg ttggatcttc tgtaaaaggt ggaacattat tagccggtta
     9841 tcagaataaa gctatcaaag ttggagaaac aatggtcgat gaaccattac atttccaagg
     9901 tggtttgaca aaacgatcat acttcaataa aaacgggcat gtaactatta atgataagca
     9961 agctttgatg cattcatcaa acgtatatat gtttaaaaca gcattaaaat tagcgggaga
    10021 cccttattat tctggtatgg ctttaccttc agacataagt tcacctgccc aaaagctaag
    10081 aagaggatta aatcaagtag gcttaggtgt gaaaacaggg atagatttac caaatgaaac
    10141 aagaggtcaa atcgaaccat taacaaataa tccaggtaat tatctagatt tatcaattgg
    10201 tcaatatgat acctatacac cattacaatt atcacaatat gtttcaacta tagcgaatga
    10261 tggttataga atacagccac acattggatt aacgattcat gaatcaacta ataaagatga
    10321 ggttggtcca ctcaagaaga aaattaatgg cactgtcttg aacaaggtta ataatactga
    10381 aaaggaaatc aaacaaattc aagaaggatt caaaatggca tttaatgata aagatggtac
    10441 tggatatgtt agttttaaag atacagtagt acctactgct ggtaaaacgg gtaccgcaga
    10501 agtgttccaa aacggagagc caagagttaa ctctacttat ataggatacg cgccaattga
    10561 tgatccaaaa ttagcgtttt caattgtata tacaaatcag cctgtaccac caccatggtt
    10621 aacaggtgga gacttaggta gagatgtaat taactactac tttaagcagt taggtaaaaa
    10681 tgataaaaat aaagacaaag acaaataaaa tttaacctga cgattgtgta gcgcatggtt
    10741 gtaaaatttt aactttgcaa atattataga tgttggtata ataataaagt cgtatttaga
    10801 aatgataagg aggaataacc atgcgcgtaa acgtaacttt agcttgtacg gaatgtggtg
    10861 acagaaacta cattacaact aagaacaaaa gaaataatcc agaacgtgtt gaaatgaaga
    10921 aattctgttc acgtgaaaac aaacaaactt tacaccgtga aacaaaataa tcattaaaat
    10981 taaatttaaa ttaacaataa aaaaggtgaa atctcaaaat aagaggtttc acctttttaa
    11041 ttatgcacac gtatgaaatg ataaaagatt ttcaattgaa ataataaatt ttagaattgt
    11101 cgcataattc atcatgacaa cataatgaca tgtattgtta ttaacgatat aatagaattg
    11161 aataaagtgg gtgataatgt gactaaaaat gagattagaa aatacatttt acataaaatg
    11221 aagaatttta ataaagctga aaagcgaaaa gcagacacat ggttaagaaa tcaatttttt
    11281 gcaactgaag aatacaaaga agcaaacgca attgcgctag ttctttcttt taatcatgaa
    11341 gtagatactt tttctattat tgaacaagcc ttaatggatc ataaacgtat ttttgtaccg
    11401 aaaatggatt atttaaatca tcaaatgact tttaaagaga tatttaatct caaagatatt
    11461 gatgtcgata ataaggggat ttactatcca acttcaaaag gtgaaacaac gaataaccta
    11521 gatttaattg ttgttcctgg tgttggattt caagacgatg gatatagaat tgggtatggt
    11581 ggtggctatt acgacaggtt tttagctaat tatcagacaa agacaataag cttattatac
    11641 gattttcaaa taacatcatt tgaaccggaa tcattcgatc aaccagtcga taaattgatt
    11701 atatatcaag cagcatagtg gaggactaaa tgaacataga caaacaattt tggaaaacaa
    11761 tatattattg gattaggtac ttgaattttg atattgtcag tagggaaaaa gatgaccaag
    11821 aaatttggtt agcccataaa agaaaaaaac aagtagtcat ttttaaacaa catatcaagt
    11881 ctacccaaga aatacgtttt gacaaagcga aagtgcttga acacaaagat gaaatagcaa
    11941 attttatttc tttcgaacca caaagttttg aattttatta ttttacagaa tcggaatttt
    12001 cagaagaaca attaaatgaa gtttcgccaa ttagaattaa attcaatgtt ataagacaca
    12061 caaaagattt gataaagcat atgccgaata tatttttggc tagacttatt tcagaagata
    12121 atgataaaaa gacatatatg ttctataaac gcaaagtatt aaccgataac tttttagata
    12181 aatatatgca gaaattttca ccggcaacat acacaataat atttgtaaat gtcttaatat
    12241 ggttatgtat gattttatat ttaaataatt tttcggatgt aaaattatta gatgttggcg
    12301 ggttagtgca ttttaatgtc gtacatggtg aatggtatcg aattgttaca tcgatatttt
    12361 tacattttag ttttgaacat atacttatga atatgctttc attatttatt tttggtaaaa
    12421 tagtcgaagc aattattggt tcatggcgga tgttaactgt atactttatt gcagggttgt
    12481 ttggaaactt tgtatcacta tcatttaata cgactacaat ttcagttggg gctagtggtg
    12541 ctatatttgg tctgattgga tcaatttttg cgatgatgta tgtttcaaaa acatttaaca
    12601 aaaaaatgtt aggacagtta ttaattgcat tagtgatatt agttggtgtt tctctgttta
    12661 tgtcaaatat aaatattgtg gcgcatattg gaggattcat tggtggttta ttaataactt
    12721 taattggcta ttactataaa gtgaatcgta atattttttg gattttacta attggtatgc
    12781 ttgttatatt tattgcactt caaattagaa tttttacaat taaagaagat aatatttata
    12841 ataaattgat caaagatgat atgactagtg gtaattatga taatgctcaa aatattgtga
    12901 agcagacaat aaataaaaat tatgccgatg atcaaacata ttatttaagt ggtatgatta
    12961 tggctactat caattctaag tccgaaggta tgacagaatg ggagcgagga cttagaatgt
    13021 ttcctaaatc aggtttatta aattttgagt tagcgatagc aaatcgttca ttaaatgatg
    13081 atgaaaaagc attaaaatat gtgcgtaaag cattaaatgc agaccctaaa aatacagatt
    13141 atattaactt agaaaaagag ttgactaaat caaatgagtc gaaaaataaa taacttttat
    13201 gatgtacaac agttattgaa aagttacgga tttctaatat attttaaaaa tccagaagat
    13261 atgtacgaaa tgattcaaca ggagatttca tcattgtatc aatatgaact gttatctaag
    13321 gaagaatatt tgaaatgtac gttgataatt aatcagagaa ggaatgaaca gaaatgacaa
    13381 aaattatttt agcagctgat gtaggcggga cgacttgtaa attaggtatt ttcacacctg
    13441 aattagaaca attacataaa tggtctattc acactgatac atctgatagt acaggatata
    13501 cacttttgaa aggaatttat gattcgtttg ttgaaaaagt aaatgaaaat aattataatt
    13561 tttcaaatgt acttggcgta ggtattggtg taccaggtcc tgttgacttt gaaaaaggta
    13621 cagtaaatgg agcagtaaac ttatattggc cagaaaaagt taatgtacgt gagatttttg
    13681 aacaattcgt tgattgtcca gtgtatgtag ataatgatgc taacatagct gctttagggg
    13741 agaaacacaa aggtgctggt gaaggtgccg atgatgttgt tgccatcaca cttggtacag
    13801 gtctaggtgg aggaattatt tcaaatggtg aaatcgtaca tggtcataat ggctctggcg
    13861 cagaaatagg tcattttaga gcagacttcg atcaacgatt taaatgtaat tgtggtcgtt
    13921 ctggatgtat tgaaacagtt gcttcagcga caggcgttgt taacttagtt aacttctact
    13981 atccgaagtt gacgtttaga tcttctatat tagaattgat taaagaaaat aaggttacag
    14041 caaaagctgt ttttgatgcg gcaaaagctg gtgaccaatt ctgtattttc attactgaaa
    14101 aggttgcaaa ctatattgga tatttatgta gtattattag tgttacaagt aatccgaaat
    14161 atatcgttct aggtggagga atgtctactg caggacctat tttaattgaa aatattaaaa
    14221 cagaatatca taatttaaca tttgcacctg ctcaatttga aactgaaatt gtacaagcga
    14281 aattaggtaa tgatgcaggt attacaggag cagcaggatt aatcaagacc tatgtattag
    14341 ataaagaggg ggtaaaataa tggctattgt tgatgtggtt gttattccag ttggaacgga
    14401 aggtccgagt gttagtaaat atattgcaga tattcagaaa aaacttcaag aatataaagc
    14461 aatgggtaaa attgattttc aattaacacc aatgaatact ctaattgaag gtgaattaag
    14521 cgatgtatta gaagttgtgc aagtgataca tgaattacct tttgataaag gtttaagtag
    14581 agtttgtaca aatatccgta ttgatgaccg acgagacaaa tctagaaaaa tgaatgataa
    14641 actaacatca gtacaaaaac atttagaaaa tagtggtgaa aacctatgag gatttcaagc
    14701 ttaactttag gcttagttga tactaatacg tatttcatcg aaaatgacaa agctgttatt
    14761 ctgattgacc cttcaggtga aagtgaaaaa attattaaaa aattaaacca aataaataaa
    14821 ccgttaaaag ctattttatt aacacatgca cactttgatc atatcggagc agtcgatgat
    14881 atagttgatc gattcgatgt cccggtttat atgcatgaag cagagtttga ttttctaaaa
    14941 gatcccgtta aaaatggggc agataaattt aagcaatatg gattaccaat tattacaagt
    15001 aaggtaactc ctgaaaagtt aaacgaaggt agcacagaaa tagaaggatt taagtttaat
    15061 gtgttacaca cacctggaca ttcaccagga agtttaacat atgtgttcga tgaattcgca
    15121 gttgttggag atacattatt taataatgga atcggacgta cagatttata taaaggtgat
    15181 tatgaaacgc tagttgattc tattcaagat aaaatatttg aattagaagg cgatttacct
    15241 ttattccctg gacatggtcc atatacgacg gttgatgatg aacaattaaa tccattttta
    15301 cacggttaaa ttgaagtaat aaaaacacct cctacgtgat tatatgtagg aggtgttttt
    15361 ttgaagattc tatttcaaga aataattaat aaagcgatag aaatgaaagc gagtgatgta
    15421 cattttattc cagttaaaaa tgaagtaagt attaaattta gaattaatga taacttggag
    15481 cagtatgaac aaattgggaa tagcatttat caaaagttat tagtttatat gaagtttcaa
    15541 gctgggcttg atgtttctac acagcaagtc gcacagagcg gtcgatatag ttaccttttc
    15601 aataaaatat attttttgag aatatcaact ttaccattgt cacttggcca agaaagttgt
    15661 gttatcagaa ttgtacctca attttttcaa ccacagaaat caacttataa attcaatgat
    15721 tttaaacacc tcatgaataa gaaacaagga ttactattgt ttagtggacc aactggttca
    15781 ggaaagagta cattaatgta tcaaatggtc tcatacgcga ataaagcctt gaatttaaat
    15841 gtaatttcta tagaggatcc tgtagagatg caaattcctg gtatcgtcca aattaatgtg
    15901 aatgataaag ctggcataaa ctatgtaaat tcgtttaaag ctattttaag atgtgatcct
    15961 gatgttattt taataggtga aatcagagat aaagatgttg ccaagtgtgt tatacaggct
    16021 agtttaagtg gtcaccttgt tctgactaca ttgcatgcaa ctgattgtaa aggtgctatt
    16081 ttaaggctat tagaaatggg catttctgta caagaattga tacaggcaac taacttaatt
    16141 ataaaccaac gacttgtaac tactattaag caacagcgac aattagtatg tgaaattcta
    16201 tctcagcaac aactccgata tttcttttcc cataatcatt cattaccatc atcatttaag
    16261 aacttagaag ataaacttga tgatatgaca aaagcaggtg tcatttgtga aactacaatg
    16321 cataaataca tttaaactac attctaagaa gcgacaatta agtaaggccc aacaaatcga
    16381 cttactttca aatttatgta atttgttgaa atatggtttc actctgtatc aaagttttca
    16441 atttttaaat cttcaaatga catataaaaa taagcaatta ggtaccacca ttctaagtga
    16501 aatttcaaat ggtgcaccat gcaatcaaat attatcactg ataggttata gcgatactat
    16561 cgtcatgcaa gtatatttgg cagaaagatt tggcaatatt atagacgttc tagaagaaac
    16621 cgtaaattat atgaaagtga atagaaagtc agaacaacga ttgttaaaga cactgcaata
    16681 ccccttaata ctagtttcta tctttattgc tatgattatt atattaaacc tcacagtaat
    16741 tccacagttt caacaattat atacttctat gaatattcaa ctatcttctt ttcaaaaaac
    16801 attgtctttt ttcattacca gcttacctac tataattgta gtaatgctca taatagtatc
    16861 tatgttggct attattatga aattaattta taacaattta aatatgctca ataagataaa
    16921 ctttgtgatg aaactaccgc taatatcagg ctatttccaa ttatttaaaa cttattttgt
    16981 aactaatgaa ttagtgttgt tttataaaaa tggtattaca cttcaatcaa tagtagacgt
    17041 ttatattaac catagtagtg atccatttag acagtttcta ggtaaatact tattaactta
    17101 ttcagaaatg ggatatggtt tgcctcaaat tttagaaaaa ctaaaatgct ttaagcctca
    17161 attaattaag tttgtgctac aaggtgaaaa gagagggaag ctagaagtag aactaaagtt
    17221 atattcgcaa atattagtaa aacaaataga agataaagcg ataaaacaga ctcagttttt
    17281 acagcctatt ttatttttga ttttaggttt atttattgtc gcaatttatt tagtaattat
    17341 gttaccaatg tttcaaatga tgcaaagtat aaaataatgg aggttaatat atgtttaaat
    17401 ttcttaagaa aactcaagcg tttacattga tagagatgct attagtgtta ttaatcatca
    17461 gtttattatt aattttaatc attccaaata ttgctaaaca aactgctcac atacaatcaa
    17521 caggttgtaa tgcacaggta aaaatggtta atagtcaaat tgaagcgtat gcattgaaac
    17581 ataatagaaa tccatcgtct attgaagact taattgcaga tggttttata aaagaagcac
    17641 aaaagacatg taaatcagga gagacaatca caattagtaa tggagaagca gttgcaaatt
    17701 agaaagcagt cagcatttac tatgattgag atgcttgtgg taatgatgtt aatcagtata
    17761 tttctacttt tgacaatgac atctaaagga ttaagcaatc ttagagtaat agatgatgag
    17821 gcaaatatca tttcttttat tactgaattg aattatatta agtcgcaagc tatagcaaat
    17881 caaggatata tcaatgttag attttatgaa aacagtgaca ctattaaagt aatagagaat
    17941 aataatatac gatttctaaa attaaaagta ggcaaaataa ttaatgttgc aaaagttgat
    18001 attattgcct ttgataaaaa agggaatatc aataaatttg gtagcataac aatttacaat
    18061 aacaattcaa tttatagaat aatattccat attgaaaaag gaagaattcg ttatgaaaag
    18121 ctataagtgt aaaggttcat tcttaataga tagtatggct ggatttttgc taattggatt
    18181 gattacatta ctattgatac caatgatgaa tcaaatgcaa gcgagtataa accataaact
    18241 acaaacaatt gatgcttcta aagtaatttt gacgactgta tctaaaatta ataaagaaga
    18301 acttaagaag ggggtaacta tagggaagta tgatattaag caaagtgacc aacaaatttg
    18361 tgctatttca aaaaatacca cttcttatca aaagacatgt atacagtatt aatgtcaaag
    18421 ctttttcgct cattgaaatg ttagtagcga tgatggttat aagtataact ttactaattg
    18481 ttccagactt aattagactt agtaaaactt ttctaattga aagtagggat ttaacaactg
    18541 tagatttcga atttttctca agagatattc tagatgattt taaaggagta gatagaaacg
    18601 atattgaaat taggcaacac cgtatcattt tacataaagg tgaaga
//
LOCUS       DALAGD010000063        18379 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.064, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000063 DALAGD010000000
VERSION     DALAGD010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18379
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.064"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(<1..81)
                     /locus_tag="O2701_001758"
     CDS             complement(<1..81)
                     /locus_tag="O2701_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCZ4293646.1"
                     /translation="MRRENILLRLIPFNDYDNKNFKMFIVI"
     gene            232..1230
                     /locus_tag="O2701_001759"
     CDS             232..1230
                     /locus_tag="O2701_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCZ4293647.1"
                     /translation="MKKVSIKDVAREAGVSVTTVSHILNHNDSRFSATTIKNVHAVSE
                     RLGYAPNKHAKQLRGSKIQTIGVILPSLTNPFFSALMQSIHDHKPSDVDLCFLTSTAT
                     DLYDNIKHLIDRGIDGLIIAQYISSPDALNNYLKKHHVPYVVLDQNDHQGYTDFVRTN
                     EYQGGQLAAQHLIELGHNHMIIVAPYDMMANMSTRVAGFVDTLRANQLPEPQIVHTEL
                     SKRGGLTIVDDIMVQSATAIFAINDELAIGILRGLIEHGISIPKDISLIGYDDIDYAA
                     YVSPPLTTVAQPITDIGKTSLTLLLQRLQHLDKSIDMIELPTTLKIRATTGYHLSN"
     gene            1462..2343
                     /gene="rbsU"
                     /locus_tag="O2701_001760"
     CDS             1462..2343
                     /gene="rbsU"
                     /locus_tag="O2701_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose transporter RbsU"
                     /protein_id="HCZ4293648.1"
                     /translation="MSIVALLIGLGPLIGWGFFPTVASKFGGKPVHQIIGATVGTLIF
                     AIILAVVTSSGLPTGTNLLFALLSGAGWGFGQIITFKAFELVGSSRAMPVTTAFQLLG
                     ASLWGVFALGNWPGIGHKIIGFTALVVILIGARMTVWSERKEASNAKNLRRAVVLLLI
                     GEFGYWLYSAAPQATSIDGLTAFLPQAMGMVIVAVIYGFMNMKAENPFRNKITWLQII
                     SGFFFAFGALTYLISAQPNMNGLATGFILSQTSVVLATLTGIYFLKQHKTSKEMVITI
                     IGLVLILVAASVTVFIK"
     gene            2358..2762
                     /gene="rbsD"
                     /locus_tag="O2701_001761"
     CDS             2358..2762
                     /gene="rbsD"
                     /locus_tag="O2701_001761"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCZ4293649.1"
                     /translation="MKKSAILNEHISKAIATIGHFDLLTINDAGMPIPNDHRRIDLAV
                     TKNLPRFIDVLATVLEEMEIQKIYLAEEIKEHNPTQLQQIKQLISSEIEIIFIPHEEM
                     KSNLAHPLNKGNIRTGETTPYSNIALESNVTF"
     gene            2790..3704
                     /gene="rbsK"
                     /locus_tag="O2701_001762"
     CDS             2790..3704
                     /gene="rbsK"
                     /locus_tag="O2701_001762"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCZ4293650.1"
                     /translation="MTNKVVILGSTNVDQFLTVERYAQPGETLHVEEAQKAFGGGKGA
                     NQAIATARMQADTTFITKIGTDGVADFILEDFKVAHIDTSYIIKTAEAKTGQAFITVN
                     AEGQNTIYVYGGANMTMTPEDVINAKDAIINADFVVAQLEVPIPAIISAFEIAKAHGV
                     TTVLNPAPAKALPNELLSLIDIIVPNETEAELLSGIKVTNEQSMKDNANYFLSIGIKT
                     VLITLGKQGTYFATKNQSQHIEAYKVNAIDTTAAGDTFIGAFVSRLNKSQDNLADAID
                     FGNKASSLTVQKHGAQASIPLLEEVNQV"
     gene            complement(3802..3936)
                     /locus_tag="O2701_001763"
     CDS             complement(3802..3936)
                     /locus_tag="O2701_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293651.1"
                     /translation="MIIIIINIAYLYAIIWKLKRLQKIVTSFITKHESTSIGLVENYF
                     "
     gene            3955..4716
                     /locus_tag="O2701_001764"
     CDS             3955..4716
                     /locus_tag="O2701_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCZ4293652.1"
                     /translation="MSKEAGHTFLAKLGKTRLRPGGKEATDWLIQQGAFSQDKQVLEV
                     ACNMCTTSIYLAHTYGCHIQGVDINKKALEKAQENISAAGLESYIQVQQANAVKLPFD
                     DNQFDIVLNEAMLTMLPIAIKEKALREYYRVLKPGGILLTHDIVIVNESHATHVVKSL
                     SAAINVNVSPQTKLGWLDLYNQAGFNHVHYHTGPMSLMTPKGLIYDEGIVGTIKIINN
                     ALKKENRPMFCKMFKTMTKLRKDMNYITFVAKKEH"
     gene            complement(5150..6586)
                     /locus_tag="O2701_001765"
     CDS             complement(5150..6586)
                     /locus_tag="O2701_001765"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCZ4293653.1"
                     /translation="MTKLPQNFMWGGALAANQFEGGYDKGGKGLSVIDVMTSGAHGKA
                     RQITESIDPNHYYPNHEGIDFYHRYKEDIALFKEMGLKCLRTSIAWTRIFPNGDEDVP
                     NEEGLAFYDRIFDELIAQGIEPVVTLSHFEMPLHLAKHYGGFRNREVVDYFVHFARVV
                     FERYKDKVTYWMTFNEINNQMDTSNPIFLWTNSGVALTENDNPEEVLYQVAHHELLAS
                     ALAVRLGKEINPKFKIGTMISHVPIYPYSCHPKDMMEAQIANRLRFFFPDVQVRGYYP
                     SYAKKMLARKGYDVGWQEGDDSILQQGTVDYIGFSYYMSTAVKHDVDTTVENNIVNGG
                     LNHSVENPHIATSDWGWAIDPDGLRYTLNVLYDRYQLPLFIVENGFGAVDEVVDGHIH
                     DDYRIEYLKAHITAAIEAVDQDGVDLIGYTPWGIIDIVSFTTGEMKKRYGLIYVDRDN
                     DGHGTMERLKKDSFYWYQQVIASNGDKL"
     gene            complement(6602..7393)
                     /locus_tag="O2701_001766"
     CDS             complement(6602..7393)
                     /locus_tag="O2701_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCZ4293654.1"
                     /translation="MSNKYKEQAQDILTAVGGVENIVDATYDTKCITIHMQHTIPSTA
                     NEVKQIVDVTSVAENDTQLVIKLNGNVDEVYQQLQRLIKNANVEESENTDNINSQDTS
                     YTPQVKVTTPILVKAPIAGRRILLKEVRDSIFREKMVGEGLAIKAHEESKVIAPFNGL
                     ISMIVPTKHAVGIQSEDGVDIVIHIGLNTVDLEGKGFKCFVKQNDHVEAGQTLLQFDQ
                     QYIQQQGYNADVIVVISNSADLGKVELTMNEIITTEDVIFKIFKN"
     gene            7542..8246
                     /locus_tag="O2701_001767"
     CDS             7542..8246
                     /locus_tag="O2701_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCZ4293655.1"
                     /translation="MLKYEHIAKQLNAFIHQSNFKPGDKLPSVTQLKERYQVSKSTII
                     KALGLLEQDGLIYQAQGSGIYVRNIADANRINVFKTNGFSKSLGEHRMTSKVLVFKEI
                     ATPPKSVQDELQLNADDTVYYLERLRFVDDDVLCIEYSYYHKEIVKYLNDDIAKGSIF
                     DYLESNMKLRIGFSDIFFNVDQLTSSEASLLQLSTGEPCLRYHQTFYTMTGKPFDSSD
                     IVFHYRHAQFYIPSKK"
     gene            complement(8354..9055)
                     /gene="lrgB"
                     /locus_tag="O2701_001768"
     CDS             complement(8354..9055)
                     /gene="lrgB"
                     /locus_tag="O2701_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like protein LrgB"
                     /protein_id="HCZ4293656.1"
                     /translation="MINHLALNTPYFGILLSVIPFFLATILFEKTNRFFLFAPLFVSM
                     VFGVAFLYLTGIPYKTYKIGGDIIYFFLEPATICFAIPLYKKREVLVKHWHRIIGGIG
                     IGTVVALLIILTFAKLAQFANDVILSMLPQAATTAIALPVSAGIGGIKELTSLAVILN
                     GVIIYALGNKFLKLFRITNPIARGLALGTSGHTLGVAPAKELGPVEESMASIALVLVG
                     VVVVAVVPVFVAIFF"
     gene            complement(9048..9491)
                     /gene="lrgA"
                     /locus_tag="O2701_001769"
     CDS             complement(9048..9491)
                     /gene="lrgA"
                     /locus_tag="O2701_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like murein hydrolase modulator LrgA"
                     /protein_id="HCZ4293657.1"
                     /translation="MVVKQQKDASKPAHFFHQVIVIALVLFVSKIIESFMPIPMPASV
                     IGLVLLFVLLCTGAVKLGEVEKVGTTLTNNIGLLFVPAGISVVNSLGVISQAPFLIIG
                     LIIVSTILLLICTGYVTQIIMKVTSRSKGDKVTKKIKIEEAQAHD"
     gene            complement(9604..10344)
                     /locus_tag="O2701_001770"
     CDS             complement(9604..10344)
                     /locus_tag="O2701_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor LytR"
                     /protein_id="HCZ4293658.1"
                     /translation="MKALIIDDEPLARNELTYLLNEIGGFEEINEAENVKETLEALLI
                     NQYDIIFLDVNLMDENGIELGAKIQKMKEPPAIIFATAHDQYAVQAFELNATDYILKP
                     FGQKRIEQAVNKVRATKAKDDNNASAIANDMSANFDQSLPVEIDDKIHMLKQQNIIGI
                     GTHNGITTIHTTNHKYETTEPLNRYEKRLNPTYFIRIHRSYIINTKHIKEVQQWFNYT
                     YMVILTNGVKMQVGRSFMKDFKASIGLL"
     gene            complement(10347..12101)
                     /locus_tag="O2701_001771"
     CDS             complement(10347..12101)
                     /locus_tag="O2701_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCZ4293659.1"
                     /translation="MLSLTMLLLERVGLIIILAYVLMNIPYFKNLMNRRRTWKARWQL
                     CIIFSLFALMSNLTGIVIDHQHSLSGSVYFRLDDDVSLANTRVLTIGVAGLVGGPFVG
                     LFVGVISGIFRVYMGGADAQVYLISSIFIGIIAGYFGLQAQRRKRYPSIAKSAMIGIV
                     MEMIQMLSILTFSHDKAYAVDLISLIALPMIIVNSVGTAIFMSIIISTLKQEEQMKAV
                     QTHDVLQLMNQTLPYFKEGLNRESAQQIAMIIKNLMKVSAVAITSKNEILSHVGAGSD
                     HHIPTNEILTSLSKDVLKSGKLKEVHTKEEIGCSHPNCPLRAAIVIPLEMHGSIVGTL
                     KMYFTNPNDLTFVERQLAEGLANIFSSQIELGEAETQSKLLKDAEIKSLQAQVSPHFF
                     FNSINTISALVRINSEKARELLLELSYFFRANLQGSKQHTITLDKELSQVRAYLSLEQ
                     ARYPGRFNININVEDKYRDVLVPPFLIQILVENAIKHAFTNRKQGNDIDVSVIKETAT
                     HVRIIVQDNGQGISKDKMHLLGETSVESESGTGSALENLNLRLKGLFGKSAALQFEST
                     SSGTTFWCVLPYERQEEE"
     gene            complement(12348..13022)
                     /gene="scdA"
                     /locus_tag="O2701_001772"
     CDS             complement(12348..13022)
                     /gene="scdA"
                     /locus_tag="O2701_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein ScdA"
                     /protein_id="HCZ4293660.1"
                     /translation="MINKNDIVADVVTDYPKAADIFRSVGIDFCCGGQVSIEAAALEK
                     KNVDLNELLQRLNDVEQTNTPGSLNPKFLNVSSLIQYIQSAYHEPLREEFKNLTPYVT
                     KLSKVHGPNHPYLVELKETYDTFKNGMLEHMQKEDDVDFPKLIKYEQGEVVDDINTVI
                     DDLVSDHIATGELLVKMSELTSSYEPPIEACGTWRLVYQRLKALEVLTHEHVHLENHV
                     LFKKVS"
     gene            complement(13162..14886)
                     /gene="tarS"
                     /locus_tag="O2701_001773"
     CDS             complement(13162..14886)
                     /gene="tarS"
                     /locus_tag="O2701_001773"
                     /EC_number="2.4.1.355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(ribitol-phosphate)
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCZ4293661.1"
                     /translation="MMKFSVIVPTYNSEKYITELLNSLAKQDFPKTEFEVVVVDDCST
                     DQTLQIVEKYRNKLNLKVSQLETNSGGPGKPRNVALKQAEGEFVLFVDSDDYINKETL
                     KDAAAFIDEHHSDVLLIKMKGVNGRGVPQSMFKETAPEVTLLNSRIIYTLSPTKIYRT
                     ALLKDNDIYFPEELKSAEDQLFTMKAYLNANRISVLSDKAYYYATKREGEHMSSAYVS
                     PEDFYEVMRLIAVEILNADLEEAHKNQILAEFLNRHFSFSRTNGFSLKVKLEDQPQWI
                     NALGDFIQAVPERVDALVMSKLRPLLHYARAKDIDNYRTVEESYRQGQYYRFDIVDGK
                     LNIQFNEGEPYFKGIDIAKPKVKMTAFKFDNHKIVTELTLNEFMIGEGHYDVRLKLHS
                     RNKKHTMYVPLSVNANKQYRFNIMLEDIKAYLPKEKIWDVFLEVQIGTEVFEVRVGNQ
                     RNKYAYTAETSALIHLNNDFYRLTPYFTKDFNNISLYFTAITLTDSISLKLKGKNKII
                     LTGLDRGYVFEEGMASVVLKDDMVMGMLSQTSENEVQILLSKDIKKRDFKNIVKLNTA
                     HITYPLNK"
     gene            complement(14919..16607)
                     /locus_tag="O2701_001774"
     CDS             complement(14919..16607)
                     /locus_tag="O2701_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCZ4293662.1"
                     /translation="MVKSKIYIDKIYWERVQLFVEGHSENLDLEDSNFVLRNLTETRT
                     MKANDVKIDGNQFVCRFNVAILDNGYYLPEDKYLLVNEQELDYIAQLNPDVINDAYQN
                     LKPEQEEEYNELETQNGKINFLLQTYLKEFRKGGISKKTVYTVTPEISSDVNEFVLDV
                     VVTTPEVKSIYIVRKYKELRKYFRKQSFNTRQFIFKAIFNTTKFFHLKKGNTVLFTSD
                     SRPTMSGNFEYIYNEMLRQNLDKKYDIHTVFKANITDRRGIIDKFRLPYLLGKADYIF
                     VDDFHPLIYTVRFRRSQEVIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKAYV
                     SSETDIPFYAEAFGIKEKNVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKVIL
                     FAPTFRGSGHGTAHYPFFKIDFERLARYCEKNNAVVLFKMHPFVKNRLNIADKHKQYF
                     VDVSDFREVNDILFITDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEPYE
                     SFVPGKIVQSFDALMDALDNEDYEGEKVIPFLDKHFKYQDGRSSERLVRNLFGS"
     gene            complement(16629..17654)
                     /locus_tag="O2701_001775"
     CDS             complement(16629..17654)
                     /locus_tag="O2701_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCZ4293663.1"
                     /translation="MINQVYQLVAPRQFEVTYNNVDIYSDYVIVRPLYMSICAADQRY
                     YTGSRDENVLSQKLPMSLIHEGVGEVVFDSKGVFNKGTKVVMVPNTPTEKDDVIAENY
                     LKSSYFRSSGHDGFMQDFVLLNHDRAVPLPDDIDLSIISYTELVTVSLHAIRRFEKKS
                     ISNKNTFGIWGDGNLGYITAILLRKLYPESKIYVFGKTDYKLSHFSFVDDVFFINKIP
                     EGLTFDHAFECVGGRGSQSAINQMIDYISPEGSIALLGVSEFPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFQDVVDLYIQYPDIVDKLALLKGQEFEIATINDLTEAFEADLSTSWGK
                     TVLKWIM"
     gene            complement(17647..18363)
                     /locus_tag="O2701_001776"
     CDS             complement(17647..18363)
                     /locus_tag="O2701_001776"
                     /EC_number="2.7.7.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribitol-5-phosphate cytidylyltransferase"
                     /protein_id="HCZ4293664.1"
                     /translation="MKYAGILAGGIGSRMGNVPLPKQFLDLDNKPILIHTLEKFILIN
                     DFEKIIIATPQQWMTHTKDTLRKFKISDERIEVIQGGSDRNDTIMNIVKHIESTNGIN
                     DDDVIVTHDAVRPFLTHRIIKENIQAALEYGAVDTVIDAIDTIVTSKDNQTIDAIPVR
                     NEMYQGQTPQSFNINLLKESYAQLSDEQKSILSDACKIIVETNKPVRLVKGELYNIKV
                     TTPYDLKVANAIIRGGIADD"
BASE COUNT         5893 a         3442 c         2794 g         6250 t
ORIGIN      
        1 aatcacaata aacattttaa aattcttgtt atcataatca ttaaaaggta ttaaccttaa
       61 taatatattc tctcgtctca accttaatcg tatacttcag acgtctgttt gtagacaata
      121 aaagtcattc acgtcttcat atgtcatcaa atgtttatca tgatatgatg aatataataa
      181 tcgggtatat aactgtatga ttaattacac aataaagaga aggaatataa tatgaaaaaa
      241 gtgtcaatta aagatgttgc tagagaagct ggtgtatcag ttacaactgt gtcacatatt
      301 ttaaatcata atgatagtcg tttttccgca acaacgataa aaaacgtaca tgctgtttca
      361 gaacgtttag gctatgcccc taataaacat gcaaaacaat tgcgcggcag taaaattcaa
      421 actattggcg tcattttgcc tagcttaaca aatccgtttt tctcagcact gatgcaaagt
      481 attcatgacc ataaaccatc tgatgttgat ttatgctttt taacatctac agcaactgat
      541 ttgtatgaca atattaaaca tttaattgat cgaggtattg acggattaat tatcgcacaa
      601 tacatatcat ccccggacgc cctaaataac tatctaaaga aacatcatgt accttatgtc
      661 gtactggatc aaaatgacca tcaaggctat acagattttg ttcggacaaa tgaatatcaa
      721 ggtggacaac ttgcagcaca acatttaata gaactcggtc acaaccatat gataattgtt
      781 gcaccatatg acatgatggc gaatatgtcg actcgtgtcg ctggatttgt cgatactttg
      841 cgcgcgaatc aattgccaga accacaaatc gtccatactg aattatctaa gcgcggtggg
      901 ctaaccattg ttgatgacat catggttcaa tctgccactg caatcttcgc tattaacgat
      961 gaactcgcta ttggcatttt acgaggacta attgaacatg gcatcagtat cccgaaagat
     1021 atctcattaa taggttatga cgacattgat tatgcagcgt acgtctcgcc acctttaact
     1081 actgtggcac aacctataac tgatattggc aaaacatctt taaccttatt acttcaacga
     1141 ttacagcact tagataaatc cattgatatg attgaattac caacgacttt aaaaattcgt
     1201 gcaacaactg gctatcatct ttcaaactaa ctacgtatct tccgaaatat actcatcatt
     1261 gttaggccct tagcgttgct ttaatgctga gggtttttaa tcataattat tttactaaga
     1321 aattaaaata ataatgtatg aatttttaaa tatgatttaa acgttttcag tttttatgaa
     1381 aacgcatgca ttttacaaat aaaaatggta cgatggcact ggtaaaacgt tttactaaaa
     1441 acaaatcatg aggtgtataa catgagcatt gttgcattac ttatcgggtt aggcccctta
     1501 attggctggg gcttcttccc aacagtcgct tcaaagtttg gtggtaaacc tgtacatcaa
     1561 attatcggtg ctactgtagg tacgttaatc ttcgctatta tattagccgt agtcacatca
     1621 agtggcttac ctactggaac caatttgcta ttcgccttat tatcaggtgc aggatgggga
     1681 ttcggacaaa tcattacatt taaagcgttc gaattagtcg gctcatctcg tgccatgcca
     1741 gtcacaacag cattccaatt attaggcgca tctttatggg gtgtctttgc attaggaaat
     1801 tggccaggca ttggtcataa aatcattgga tttacagctt tagtcgttat tctaattgga
     1861 gcgcgtatga cagtttggag tgaacgcaaa gaagcaagta acgccaaaaa tttacgtcgt
     1921 gcagtggtac ttctgttaat tggtgaattt ggatactggt tatattcagc tgcaccgcaa
     1981 gcaacttcta ttgatggcct aactgccttt ttacctcaag caatgggtat ggtaattgtt
     2041 gcagtcattt atggctttat gaatatgaaa gcagagaatc cattccgtaa taaaattacg
     2101 tggttacaaa ttatttcagg tttcttcttt gcatttggtg ctttaacata tcttatttca
     2161 gcacaaccta atatgaatgg tttagcaact ggatttattc tttctcaaac atccgttgtg
     2221 cttgctacat taactggtat ttatttctta aaacaacata aaacgtcaaa agaaatggtt
     2281 attacaatca tcggcttagt actcatttta gtagccgctt ctgttacagt atttataaaa
     2341 taaggagtgt agatgtcatg aaaaaatcag ctattttaaa tgaacatatt tcaaaagcaa
     2401 tcgcgacaat tggtcatttt gatttattaa cgattaatga cgctggcatg ccaattccaa
     2461 atgatcatcg tcgtatcgac ctagctgtaa ctaaaaactt accacgcttt attgatgtct
     2521 tagctacagt gttagaagaa atggaaatcc aaaaaatata cttagcagaa gaaataaaag
     2581 aacataaccc tacacaattg caacaaatta aacaattgat ttcatcggaa atcgaaatca
     2641 ttttcattcc tcacgaagaa atgaaaagta acttagctca cccattaaat aaaggtaata
     2701 ttcgtactgg tgaaacaacg ccctactcta atattgcatt agaatcgaat gttacttttt
     2761 aaaagttata acttgaaagg agcgtacaca tgaccaacaa agttgttatt ttaggttcaa
     2821 cgaatgtcga tcaattttta acagttgaaa gatatgcaca accaggcgaa acattacatg
     2881 ttgaagaagc acaaaaagca ttcggcggag gtaaaggtgc caaccaggct attgccactg
     2941 cacgcatgca agcagacaca acatttatta ctaaaattgg cactgatggc gttgctgatt
     3001 tcatcttaga agattttaaa gtagctcata ttgatacatc atatattatc aaaacagctg
     3061 aagcaaaaac gggccaagcc tttatcactg tgaatgcaga aggacaaaac accatctatg
     3121 tttatggtgg tgcgaatatg acgatgacac ctgaagatgt tattaacgca aaagacgcta
     3181 taatcaatgc agactttgtc gttgcacaat tagaagtacc catcccggct attatatctg
     3241 catttgaaat tgccaaggca catggtgtga cgacagtatt aaatcctgca ccagcgaaag
     3301 cattacctaa tgaattatta tcattaatcg atattattgt gccaaacgaa acagaagccg
     3361 aattgttatc tgggattaaa gtaactaatg aacaatctat gaaagacaat gccaattact
     3421 ttttatctat aggcattaag actgttttga ttacgctagg taagcaaggt acatattttg
     3481 ctactaaaaa tcaaagccaa cacatcgaag cttataaagt aaatgcgatt gatacaactg
     3541 ctgcaggcga cacatttatt ggtgcatttg tcagtcgctt aaacaagtcg caagataact
     3601 tagcagatgc tattgatttt ggtaataaag cgagctcact cactgtacaa aaacacggcg
     3661 cgcaagcatc tattcctcta ctagaagaag taaatcaagt ttaaatgaat caaacacagc
     3721 tatgatatga aggtttagca tataacatgc aacattcgta tatcatggct gtgctttttt
     3781 atctttataa aacatcatct attagaaata attttccact aaacctatgc ttgttgactc
     3841 atgtttagtt ataaatgaag tgacaatttt ttgtaatctt tttaacttcc aaattatcgc
     3901 atataaatat gctatattaa tgataataat tatcaattaa aaggaggtta tgctatgtct
     3961 aaagaagctg gtcatacatt tttagctaaa ttaggaaaaa ctcgtctacg ccccggtggt
     4021 aaagaagcaa cagattggtt aatacaacaa ggggcatttt cacaagataa acaagtgtta
     4081 gaagtggcat gtaatatgtg cacaacatct atttatctag ctcatacata tggctgtcac
     4141 attcaaggcg ttgatataaa taagaaagca ttagaaaaag cacaggaaaa catttcagca
     4201 gcaggtcttg aatcatatat tcaagttcaa caagcgaatg ctgttaaatt gccctttgat
     4261 gacaatcaat tcgatatcgt tttaaatgaa gcaatgttaa caatgttacc catcgccata
     4321 aaggaaaaag cattacgcga gtactaccga gtcttaaagc ctgggggtat cttgttaaca
     4381 catgatattg tcatcgttaa tgaatcacat gccacacatg ttgttaaatc attatctgca
     4441 gcaattaatg tcaatgtctc accgcagacg aaacttggct ggttagattt atataatcaa
     4501 gctggtttta atcatgtgca ttatcatact ggtccaatga gtttaatgac accaaaaggt
     4561 ttaatttatg acgaaggtat tgttggaact ataaagatta tcaacaatgc tttgaaaaaa
     4621 gaaaatcgac caatgttttg taaaatgttt aaaacgatga ctaaattgcg taaagatatg
     4681 aattatatta cttttgtcgc taaaaaagag cactaaatat aatgccacta actgtacttt
     4741 gtatctatgt ttgactatca ctttaatttc tttgtgacac taatcatcta cttaacaata
     4801 tcgttatcgt tgattagtaa gtcatcaatt ttggttaaag actttcataa acactcaaac
     4861 attaacacta tacatagtta gtggcattat ttttttctta aaattttaac atcacgggat
     4921 tggaacagaa atgatatttt cgcaaaattt atttctgtcc ttccccaact tgcattacct
     4981 gtagaatttc ttttcgaatt tctctgtgtt ggggccccgc caacttgcat tacctgtaga
     5041 atttcttttc gaaattcttt gtgttggggg cccgccatta taacactgta ggtcctacta
     5101 tattaattta tgtctcgggc tctaacccta aaatacttat aatatacctt tataatttat
     5161 ctccatttga tgctatcact tgttgatacc aatagaacga atcttttttc aagcgttcca
     5221 tcgtgccatg accatcatta tctcgatcaa catatattaa accatagcgt ttcttcattt
     5281 caccggttgt aaatgaaaca atatcaatga ttccccacgg tgtataaccg attaaatcta
     5341 caccatcttg atcaactgct tctatcgctg ctgtaatatg tgcttttaaa tattcaatgc
     5401 gataatcatc atgaatatgt ccatctacca cttcatcaac tgcaccaaaa ccattttcca
     5461 caataaaaag tggtaactga taacgatcat ataacacatt caatgtatat cttaagccat
     5521 ctggatcaat cgcccaaccc caatcactcg ttgcgatatg cggattctcc acagaatgat
     5581 tcaaaccacc gttgacgatg ttgttttcaa ctgtagtatc aacatcatgt tttacagccg
     5641 tagacatgta ataactaaag ccaatataat caaccgtgcc ctgctgtaaa atactgtcgt
     5701 ccccttcttg ccatccaaca tcatatcctt ttcgtgccaa cattttttta gcatagcttg
     5761 gataataacc tctcacttgg acatccggga aaaagaaacg taagcgattc gcaatttgtg
     5821 cttccatcat atctttcgga tgacacgaat atggataaat gggtacatgt gaaatcattg
     5881 ttccaatctt aaacttcgga ttaatctctt taccaagacg aactgctaaa gcactggcta
     5941 aaagttcatg atgtgctact tgatacaaga cttcttcagg attatcattt tctgtcaatg
     6001 ctaccccaga attcgtccat aaaaagatag gatttgatgt gtccatctga ttattaattt
     6061 cattaaacgt catccaatat gtaactttat ctttatatct ttcaaataca acacgcgcaa
     6121 aatgcacaaa ataatcgaca acttctctat ttctaaatcc accataatgt ttcgctaaat
     6181 gaagtggcat ctcaaaatgt gataacgtca caacaggttc aataccttgt gcaattaatt
     6241 catcaaagat acgatcataa aaggcgagtc cttcttcatt tggcacatct tcatccccat
     6301 tcggaaagat acgtgtccac gcaatcgacg tacgtaaaca tttcaatccc atttctttaa
     6361 acaaggcaat atcttcctta taacgatgat aaaaatcaat accttcatga tttggataat
     6421 agtgattggg atctatagat tctgtaatct gacgtgcttt gccatgtgca ccactcgtca
     6481 taacatcaat tacacttaac cctttaccac ctttatcata tccaccttca aattgatttg
     6541 cggcaagagc gccaccccac atgaaatttt gcggtaattt tgtcattatt acaacacact
     6601 cctagttttt aaatatttta aaaataacat cttcagtcgt aatgatttca ttcattgtca
     6661 gttctacttt tcctaaatcg gcagagttgc taataacgac aataacgtca gcattgtagc
     6721 cttgttgttg tatatattgc tggtcgaatt gcaacaacgt ttgccctgct tcaacatgat
     6781 cattttgctt tacaaagcac ttgaaccctt taccttccaa gtcaactgta ttcaagccaa
     6841 tatgaatgac tatgtccaca ccgtcttctg attgaatacc aactgcatgc ttagttggta
     6901 caatcataga tattaaacca ttgaacggtg cgattacttt ggattcttca tgagctttga
     6961 ttgctaagcc ttcacctacc attttctctc taaaaattga atctcttact tctttaagta
     7021 aaatacgacg accagcgatt ggtgctttca ctaaaattgg tgttgttact tttacttgag
     7081 gtgtataact tgtatcttga ctattaatat tgtcagtatt ctcactctct tcgacattag
     7141 cattcttaat taatcgctgt aattgctgat acacttcatc gacatttcca tttaatttta
     7201 tgactaactg cgtatcattt tctgctacag atgtcacatc aactatttgt ttcacttcat
     7261 ttgctgtaga aggaattgta tgttgcatat gaattgtaat gcacttcgta tcatacgttg
     7321 catcaacaat gttttcgaca ccacctacag ctgtaagaat gtcttgggct tgttctttat
     7381 atttattgct cataaatatt accccctcaa cttgatagta gaatgaaaat gtatttactt
     7441 acctccaatt ataattgtac cggttcaatt tgtaaacgcc gatacaatta taatattttg
     7501 tgctataata attacagaca aagtgaaacg aggacagaat attgttaaag tatgaacata
     7561 ttgctaagca acttaatgcg tttatacatc aatctaattt caaacccggt gataaattgc
     7621 caagcgtgac gcaattaaaa gaacgttatc aagtaagtaa gagtactatc attaaagcat
     7681 taggcttatt ggaacaagat ggtttgatct atcaagcaca aggcagtggt atttatgtga
     7741 gaaatattgc tgatgccaat cgtatcaacg tctttaagac taatggtttc tctaaaagtt
     7801 taggtgaaca ccgaatgaca agtaaggtac ttgtttttaa ggagattgca acgccaccta
     7861 aatctgtaca agatgagctc caattaaatg cagatgatac cgtctactat ttagagcgat
     7921 taagattcgt ggacgatgat gttttatgta tcgaatattc ttattatcat aaagaaatcg
     7981 tgaaatattt aaatgatgat attgctaagg gctctatctt cgactattta gaatcaaaca
     8041 tgaaacttcg tattggtttt tcagatattt tctttaatgt agatcaactc acttcaagtg
     8101 aagcttcatt actacaattg tctacaggtg aaccatgttt acgttaccac cagacttttt
     8161 atacaatgac tggcaaaccc tttgattcat ctgacatcgt atttcattat cgtcatgcac
     8221 agttttatat tcctagtaaa aagtaataaa tacataaaaa cgtctatatc ccagttataa
     8281 actggagtat agacgttttt ttacgataat aacaatggct caaattgcta ttatcttgct
     8341 taggtttttc gttttagaag aatattgcta caaagacagg cacaactgct acaacaacta
     8401 caccaactaa cactaaagct atacttgcca ttgattcttc tacaggtcct aattctttgg
     8461 ctggtgctac acctaatgtg tgaccacttg ttccaagtgc taatcctcgg gcaatagggt
     8521 tagtaattcg gaaaagcttt aagaatttat tacctagggc ataaataatg acaccattta
     8581 aaataactgc taatgatgtt aattctttta taccaccgat accagctgat actggtaacg
     8641 caatcgctgt agttgctgct tgaggtaaca ttgataaaat aacatcattg gcaaattgtg
     8701 ctaacttcgc aaaagttaaa ataattaata acgctacaac tgtaccgata ccaatacctc
     8761 cgatgatacg atgccaatgt ttaacaagca cttcacgctt tttatataac ggaatcgcaa
     8821 aacagattgt tgccggttct aagaagaagt aaataatgtc tccacctatt ttgtaagtct
     8881 tatacggaat gcctgttaaa tagaggaagg ccacaccaaa taccatactg acaaatagcg
     8941 gtgcgaataa gaagaaacga ttagtttttt caaataatat ggtcgctaag aaaaatggta
     9001 taacggataa cagtattccg aagtaaggtg tgtttagtgc taagtggtta atcatgagct
     9061 tgtgcctcct ctattttgat cttttttgtg actttgtcac ctttagatct cgaagtaact
     9121 ttcataataa tttgtgtgac atagccagta caaataagta atagtattgt tgagacgatt
     9181 attagtccaa tgattaaaaa tggtgcttgg ctaatgacac ctaaagagtt aacaactgag
     9241 ataccggctg gtacgaagag taagccaatg ttatttgtta gtgtcgttcc tactttttcg
     9301 acttcgccta acttaacagc accagtacat aataatacaa ataataatac taaaccgatt
     9361 actgatgcag gcataggaat tggcataaat gattcaatta ttttcgatac aaagagtact
     9421 aaagcaatta caatgacttg gtgaaaaaag tgtgctggtt ttgatgcgtc tttttgttgt
     9481 ttcacgacca ttgcctccta cgtttgattt aactaaagta tagatggctc acttcgattt
     9541 gcgtgatttt tagtccgaaa tacaaaatat cataggtaaa atgcataaaa aaaaggatta
     9601 ctgttaaagt aatcctatcg acgctttaaa atctttcata aatgaacgtc caacttgcat
     9661 cttgacacca tttgtcaata ttaccatata agtatagtta aaccattgtt gcacttcttt
     9721 aatgtgtttc gtgttaataa tatatgaacg atgaatacgt ataaaataag tgggattcaa
     9781 tcgtttttca taacgattca atggctctgt tgtttcgtat ttatgattcg ttgtatgtat
     9841 ggttgtaata ccattatgtg tgccaatccc aataatattt tgttgcttta acatgtgaat
     9901 tttatcgtca atttcaacag gtaagctttg atcaaaattc gccgacatat cattcgcaat
     9961 tgcacttgcg ttattatcat ctttggcttt agtcgcacgc actttattga ctgcttgttc
    10021 aatacgtttt tgaccaaacg gtttcaaaat atagtctgtc gcatttaatt caaatgcctg
    10081 tactgcgtat tggtcatgtg cagttgcaaa aataatcgca ggtggctctt tcatcttttg
    10141 aatcttagct cctaattcga tcccattttc atccattaaa ttgacatcta aaaatataat
    10201 gtcatattga ttgatcagta gtgcttccaa tgtttctttt acattttctg cctcattaat
    10261 ttcttcaaaa ccaccaattt catttaataa atatgttaat tcattacgtg ctaatggctc
    10321 atcatctatg attaatgctt tcatatttat tcctcctctt gtctttcata aggaagtaca
    10381 caccaaaaag tggtaccgct cgatgtcgat tcaaattgta atgctgcgga ttttccaaat
    10441 aatcctttta ggcgtaagtt taaattttct aaagcactac cagttccaga ctctgattct
    10501 acagatgttt ctcccaacaa atgcatttta tctttagaaa taccctgacc attatcttgt
    10561 acaataatac gtacatgtgt tgcagtttct ttaatcactg acacgtcaat atcgttacct
    10621 tgctttcgat tcgtaaacgc atgtttgatg gcattttcaa ctaaaatttg aattaaaaat
    10681 ggtggtacaa gcacatcgcg atatttgtct tcaacattaa tattgatatt aaatcttcct
    10741 ggataacgtg cttgttcgag tgataagtat gcacgcactt gacttaactc tttatctaaa
    10801 gtaatcgtat gttgcttaga gccttgtaaa ttcgctctga aaaaataact caattctaat
    10861 agtaactctc gtgccttttc gctatttatt ctaactaaag ctgagatcgt gttaattgaa
    10921 ttgaagaaaa aatgtggact cacttgtgcc tgtaatgact taatctcagc atctttcaat
    10981 aacttacttt gcgtttcggc ttcaccaagt tcaatttggc tactaaaaat atttgccaat
    11041 ccttctgcaa gttgacgttc cacaaaagtt aaatcattag ggtttgtaaa atacatcttc
    11101 aatgtaccga cgatagaacc atgcatctca agtggtatca cgatagctgc tctaagcggg
    11161 caattcggat gactacaacc aatctcttct ttagtatgaa cttctttcaa ctttcctgat
    11221 ttcaatacat ctttagacag acttgttaat atttcatttg ttggtatgtg atgatcacta
    11281 cctgcaccta catgcgataa gatttcattt ttgcttgtaa ttgctacggc agatactttc
    11341 attaaatttt taataatcat cgcaatttgc tgtgccgatt ctctattcaa tccttcttta
    11401 aaatacggca atgtctggtt catcaattgc agtacatcat gtgtttgaac agccttcatt
    11461 tgctcctctt gctttaatgt tgaaatgata atagacataa aaatcgccgt accaacgcta
    11521 ttaacaataa tcattggtag tgcaattaat gatatgaggt caaccgcata tgctttgtcg
    11581 tgggaaaatg ttaaaatgct caacatttga atcatttcca taacaattcc aatcatggca
    11641 cttttcgcaa tactcgggta acgcttgcgt ctttgagctt gtaagccaaa ataaccagca
    11701 attataccaa taaatataga tgagataaga taaacttgtg catccgcccc acccatatac
    11761 actctgaaaa tacctgaaat aacgccaaca aatagaccta caaaagggcc accaactaat
    11821 cctgcgacac ctatcgttaa tacacgtgtg ttagctaaag atacatcatc atctaaacgg
    11881 aagtacacac ttcctgacaa actatgttga tgatcgatga cgataccagt taaattagac
    11941 attaaggcaa acaaactgaa aataatacat aattgccaac gtgctttcca tgtacgtcga
    12001 cgattcatta agtttttaaa atatggaata ttcatcaaca cataggccaa aataataatt
    12061 aaacctacac gctcaagtaa taacattgtt agcgatagca cgatagcacc tcagtgaatt
    12121 ataattatgt ttattttatc ataatagtgc atgtaaaatt catgaataaa ttattaaaaa
    12181 aacacgctac tatttaatta tctataccat taaattttca tatcaatttt tatgctaagt
    12241 tgaccatttt tcttttcaaa taatgctgtt tatgtcacta ttatgacttt tatacatagc
    12301 gaaaaactgc tggacttatt tttgccaaca gtttctaatc gcgttattta tgatactttt
    12361 ttaaataata cgtgattctc taaatgtacg tgttcatgtg ttaacacttc aagtgctttt
    12421 aatctctgat aaacaagtcg ccaagtacca cacgcttcta tcggaggttc ataactagat
    12481 gttaattcgc tcatttttac taacaattct cccgttgcaa tgtggtctga aactaaatca
    12541 tctatcacag tattaatatc gtctactacc tcaccttgct catatttaat gagttttgga
    12601 aaatcgacat cgtcttcttt ttgcatatgc tctaacatgc catttttaaa tgtatcgtat
    12661 gtttctttta actcaactaa atatggatga ttaggtccat gtactttcga taacttcgtc
    12721 acataaggtg ttaaattttt aaattcttct cttagaggtt catgatatgc tgattgaata
    12781 tattgaataa gtgatgaaac atttaaaaat ttaggattta acgaacctgg tgtattcgtt
    12841 tgttcaacgt cgttgagacg ctgtaataat tcgttcaaat ctacattttt cttttctaag
    12901 gctgctgctt ctatacttac ttgtccgcca caacaaaaat ctattcccac acttctaaaa
    12961 atatccgctg ctttcggata atcagttact acatctgcta ctatgtcatt tttatttatc
    13021 attatagata acctcctcat ttccagtgta tacaatttaa tgtatatgaa aatgaaaatc
    13081 agggaaatgt cataatcatg tcataaacag caaaataaag ccctcaaatc atgttggctc
    13141 acaatgattt gagggcattt attatttatt tagtggataa gtgatatgtg cagtgtttaa
    13201 cttaacaata tttttgaagt ctcgcttttt aatatctttg ctaagtaaga tttgcacttc
    13261 gttttctgat gtttggctta acattcccat caccatgtcg tcttttagta cgacactagc
    13321 cataccttct tcaaatacat aaccacgatc cagaccagtt aaaatgattt tgtttttacc
    13381 ttttaacttc aatgagattg aatccgttaa tgtaatagct gtaaagtata acgaaatgtt
    13441 attaaagtct tttgtgaaat acggtgttaa tctataaaaa tcattattca aatgaattaa
    13501 tgcacttgtt tctgcagtat atgcatattt attacgttga ttaccaacac gcacttcaaa
    13561 tacttccgta cctatttgga cttctaagaa aacatcccaa attttttctt taggtaaata
    13621 cgctttaata tcttctaaca taatgttaaa acgatattgt ttattcgcat tgacacttaa
    13681 aggtacatac attgtgtgct tcttgtttcg tgaatgtaat ttaagtctga catcataatg
    13741 tccttcgcca atcataaatt catttaacgt tagctctgta acaattttat gattatcaaa
    13801 tttaaatgct gtcattttca cttttggctt agcgatatca atgcctttaa agtatggttc
    13861 gccttcattg aattgaatgt ttaatttacc atctacaata tcaaaacggt agtattgacc
    13921 ttgacggtaa ctttcctcca cagttctata gttgtctata tctttcgctc tcgcgtagtg
    13981 caacaatggt cgtaatttac tcatcaccaa tgcatctaca cgttctggaa ctgcttgtat
    14041 aaagtctcct agagcattaa tccattgtgg ttgatcttct agtttaactt taagtgagaa
    14101 gccattcgta cgagaaaaac taaaatgacg atttaaaaat tctgctaaga tttgattttt
    14161 atgggcttct tctaaatctg catttaatat ttctacagca atcaatctca tgacttcata
    14221 aaagtcttca ggtgaaacat acgcactact catatgttca ccttcacgct ttgtagcata
    14281 ataatacgct ttatcactta acacactgat tcgatttgca tttaaatatg ctttcattgt
    14341 aaataattga tcttctgcac tctttaattc ttctggaaaa taaatgtcat tatcttttag
    14401 taatgctgtt ctatagattt tagtcgggct taaagtatag ataattcttg aatttaacaa
    14461 agtaacttca ggtgctgttt ctttaaacat agattgtggt acaccacgac cattaacacc
    14521 tttcatttta atcaataaga catctgagtg atgttcatca ataaatgctg ctgcatcctt
    14581 taaagtctct ttgtttatat agtcatcgga gtccacaaat aatacaaatt caccttctgc
    14641 ttgttttaac gccacatttc taggtttacc tggaccacca gaatttgttt cgagttgact
    14701 tactttcaag ttcaatttat tgcgatactt ttcaactatt tgtaacgttt gatctgttga
    14761 acagtcatca actacaacca cttcaaattc agttttcgga aaatcttgtt tcgcaaggct
    14821 attaagtaat tctgttatat acttttctga attgtatgtt ggaactatta ctgaaaattt
    14881 catcattata cctctcccac tttgactact atataaactt agctaccaaa taaatttctg
    14941 actaaacgct cacttgatcg gccatcttga tatttaaaat gtttatctaa gaatggaatg
    15001 actttttctc cttcataatc ttcattgtcc aaggcgtcca ttaatgcgtc aaatgattgc
    15061 acaattttac ctggaacaaa tgattcatat ggttcataaa aatcacgcgt cgtaatataa
    15121 tcttctaaat caaatgcata gaaaatcatt ggctttttaa atactgcata ttcatatatt
    15181 aaagatgaat agtcactaat taataaatct gttatgaaca gtatatcatt aacttctcta
    15241 aagtcagaaa cgtcaacaaa atattgttta tgtttgtctg caatattaag tctatttttc
    15301 acaaatggat gcattttaaa taatacaacc gcgttatttt tttcgcaata tcttgctaaa
    15361 cgttcaaaat caattttgaa aaatgggtaa tgtgctgtac catgaccact acctctaaat
    15421 gttggtgcga aaagaatgac tttcttacct ttaataattg gtaattcatc ttccatctct
    15481 tgtttgatct gtgtcgcata agcttcatca aatagtacat cagtacgtgg aacacctgta
    15541 ggcactacat ttttctcttt aataccaaat gcttcagcgt agaatggaat atcggtttca
    15601 gatgatacat aagcttttgt atagctacga tgatttaatg aatcaataaa tggtccaccc
    15661 tttttaccag tacgactaaa gccaactgtt ttaaaggcac caacggcatg ccatacttga
    15721 ataacttctt gagaacgtct aaaacgcact gtataaatca atgggtgaaa gtcatcaaca
    15781 aagatgtagt ctgccttccc aagtaaatat ggcaatctaa acttgtcgat gatgccacgt
    15841 ctatctgtaa tattcgcttt aaaaacagtg tgaatatcat actttttatc taaattttga
    15901 cgtaacattt cgttatagat gtattcaaag tttccagaca tcgttggtct agagtctgat
    15961 gtgaacaaca ccgtattccc ttttttcaag tggaaaaatt tcgtcgtatt aaatatcgct
    16021 ttaaaaataa attgtcttgt attaaatgat tgtttgcgga aatacttacg taattcttta
    16081 tatttacgaa cgatataaat acttttaact tccggagtcg ttacaacaac atcaaggaca
    16141 aattcattaa catcgctaga aatttcaggt gtaacagtat aaaccgtttt cttcgaaatg
    16201 ccgccttttc taaattcttt taggtaagtc tgcaataaga aattgatttt accattttgt
    16261 gtttctaatt cgttgtattc ttcttcttgt tctggcttta gattttgata tgcatcatta
    16321 atcacatctg ggtttaactg tgcaatataa tcaagttctt gctcattcac taataagtac
    16381 ttatcttcag gtaagtaata accattatct aagatagcta cattgaaacg acaaacgaat
    16441 tgattcccat ctattttgac atcattcgcc ttcattgtac gtgtctcagt taaatttctt
    16501 aatacaaaat tactatcttc taaatctagg ttttcactat gtccttcaac gaataactga
    16561 acacgttccc aatagatttt atctatatat atcttacttt taaccaacgt taattcatcc
    16621 ttttctattt acataatcca ttttaatact gttttacccc aagatgtaga caggtctgct
    16681 tcaaaagctt ctgtaagatc attaattgtt gcaatttcaa attcttgacc ttttaacaac
    16741 gctaatttat ctacaatatc tgggtattga atgtataagt ctacaacatc ttggaaatct
    16801 tttgaaccac ttcgactact accaatcaac gttagtcctt tttccaatac tagacgtgta
    16861 ttaacttcta ctgggaactc acttacacct aacagtgcaa tgcttccttc tggtgaaatg
    16921 taatcgatca tttgatttat ggctgattga ctaccgcgac cacccacaca ctcaaatgca
    16981 tgatcaaatg ttaagccttc aggtatttta ttaataaaga agacatcatc aacaaatgag
    17041 aagtgactca atttataatc tgttttacca aagacatata ttttagactc tggatataat
    17101 ttacgtaata aaatggctgt aatgtaacct aagttaccat caccccaaat accaaatgta
    17161 tttttatttg aaatagattt cttttcaaaa cgacgaatag catgcaaact tactgttaca
    17221 agctctgtat atgaaataat acttaaatca atatcatcag gtagtggtac agctctatca
    17281 tgatttagca acacaaaatc ttgcataaac ccatcatgtc cacttgatct gaagtagctc
    17341 gattttaaat agttttcagc aatgacatcg tctttttctg tcggcgtatt cggtaccata
    17401 actacttttg tacctttatt aaacacacct ttactgtcaa atacgacctc accaacacct
    17461 tcatgaatta aagacattgg caatttctga gataagacat tctcatcacg gctaccagta
    17521 taatatcttt gatcggcagc acaaattgac atatataaag gacgtacaat gacatagtca
    17581 ctgtaaatat ctacgttgtt atacgtaact tcaaattgtc taggtgcaac taattgatat
    17641 acttgattaa tcatcggcaa taccacctcg aataatagca ttcgctactt ttaaatcgta
    17701 aggtgttgtt actttaatgt tatataactc accttttaca agtcgaaccg gtttgtttgt
    17761 ttctacaata atcttacaag catcagataa aatactcttt tgctcatcac tcaactgtgc
    17821 atagctttct tttaataaat taatattaaa cgattgaggt gtttgacctt ggtacatttc
    17881 attacgcact ggaattgcat caatcgtttg attatcttta gatgtaacaa tcgtatctat
    17941 agcatcaatc actgtatcta ctgcaccgta ctctaaagca gcttgaatat tttctttaat
    18001 aatacgatgc gttaaaaatg gtctaactgc atcatgtgtc acaatgacat cgtcatcgtt
    18061 aataccattt gttgattcaa tatgtttaac gatattcata attgtatcgt tacgatcgct
    18121 accaccttga atgacttcaa ttctttcatc agaaattttg aattttctaa gtgtatcttt
    18181 cgtatgcgtc atccattgtt gtggcgtcgc gataataatt ttttcaaaat cattaattaa
    18241 aataaatttt tctaatgtat ggattaaaat cggtttgttg tctaaatcta aaaattgttt
    18301 aggtaaaggt acgttaccca ttcttgagcc tatacctcca gctagaatac cagcgtattt
    18361 cataaaatac ttcctccat
//
LOCUS       DALAGD010000064        18151 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.065, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000064 DALAGD010000000
VERSION     DALAGD010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18151
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.065"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..677
                     /locus_tag="O2701_001777"
     CDS             <1..677
                     /locus_tag="O2701_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCZ4293665.1"
                     /translation="IKKSFEKTLSMYPIKNLEDLYDKEGYRDDEFDKNDKGTWIIGSE
                     MVVQPKGERMKSKGMVLYMNRNTKTTTGKYIVSETLHDEDGRPKSKDKEYPVKMVDNK
                     IIPTKGIKDENIKKEIENFKFFAQYGSFKDLSKYKDGDISYNPEVPSYSAKYQLTNDD
                     YNVKQLRKRYKIPTNKAPKLLLKGSGDLKGSSVGYKDIEFTFVEKKGENTFFTDSLHL
                     EPSEDK"
     gene            771..3008
                     /locus_tag="O2701_001778"
     CDS             771..3008
                     /locus_tag="O2701_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCZ4293666.1"
                     /translation="MQRDYLIRVETESMSDFKRLNSLMIGFVIKGEAHIYDENNMTQC
                     NSGDIFIINHRDLYRFQLQQDGIICYIQFQMKYLADKFDDVHCLYFHLTDATTTKNIH
                     QLRNIMARLVSTHIRHNELSKLTEQQLVIQLLMHMIHYVPRTYHSNQSILNDDKVNQV
                     CDYIELHFHEDLSLSELSEYVGWSESHLSKKFTESLGVGFHHFLNTTRIEHAKLDLTY
                     TDETITDIALQNGFSSAASFARTFKHFTHQTPKQYRGDRPAITENQQSAQHDYHDREL
                     ILLLNDYIEEMNHFIEDIEKMNYKEIAFQPTNHQLNQFNHIIQVGYLRNLLNTQYQSQ
                     LLTCHHDFQVNEVLAYDVIPYIMKKLNAPFTYDAEISNIFYDIDLCLDFLLDHNFSLT
                     MHLDQYDSRDYIDAFKIFIHHVALHVSHRKDLKFNLYVTTLHTSLIEMIDYFKALFPN
                     GGLYIHLDQATERHLPLLKRLEPHIDHFVFDANSNDAVDFNKMNDDEFKTASQMIINK
                     TNYLIDLMHRHRLKRPLILLNWNTLTGDTFITNGEYFRGGIIIEQLLKLSSKVEGIGY
                     WLNYDLHVSHCKNERDYMNSIELFHQYNGKRPVYFTALLFNKLTSNILYSDDTCIVTG
                     TDSNFQILLYDAKHFNPYLALDNQMNMRATEMIHLNINALEEGMYKIKHFTLDKENGA
                     LFNLWRKHHTIHGMDKDSIDYVNRMSFPKLEVYDIDITDTLALNIKMITNGIHLIEVK
                     RYPSS"
     gene            3159..4337
                     /locus_tag="O2701_001779"
     CDS             3159..4337
                     /locus_tag="O2701_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCZ4293667.1"
                     /translation="MNQQLIETLKSKEGKMIEIRRYLHQHPELSFHEDETAKYIAEFY
                     KGKDVEVETNVGPRGIKVTIDSGKPGKTLAIRADFDALPITEDTGLSFASQNKGVMHA
                     CGHDAHTAYMLVLAETLAEMKDSFTGKVVVIHQPAEEVPPGGAKTMIENGVLDGVDHV
                     LGVHVMSTMKTGKVYYRPGYVQTGRAFFKLKVQGKGGHGSSPHMANDAIVAGSYFVTA
                     LQTVVSRRLSPFETGVVTIGSFDGKGQFNVIKDVVEIEGDVRGLTDATKATIEKEIKR
                     LSKGLEDMYGVTCTLEYNDDYPALYNDPEFTEYVAKTLKEANLDFGVEMCEPQPPSED
                     FAYYAKERPSAFIYTGAAVENGEIYPHHHPKFNISEKSLLISAEAVGTVVLDYLKGDN
                     "
     gene            4339..5727
                     /locus_tag="O2701_001780"
     CDS             4339..5727
                     /locus_tag="O2701_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ4293668.1"
                     /translation="MNETYRGGNKLILGIVLGVITFWLFAQSLVNVVPNLQQSFGTDM
                     GTISIAVSLTALFSGMFVVGAGGLADKIGRVKMTNIGLLLSIIGSALIIITNLPALLI
                     LGRVIQGVSAACIMPSTLAIMKTYYQGAERQRALSYWSIGSWGGSGICSLFGGAVATT
                     MGWRWIFIFSIIVAVLSMLLIKGTPETKSEITNTHKFDVAGLIVLVVMLLSLNVVITK
                     GAALGYTSLWFFGLIAIVIVAFFIFLNVEKKVDNPLIDFKLFENKPYTGATISNFLLN
                     GVAGTLIVANTFVQQGLGYTALQAGYLSITYLIMVLLMIRVGEKLLQKMGSKRPMLLG
                     TFIVVIGIALISLVFLPGIFYVISCVVGYLCFGLGLGIYATPSTDTAISNAPLDKVGV
                     ASGIYKMASSLGGAFGVAISGAVYAGAVAATSIHTGAMIALWVNVLMGIMAFIAILFA
                     IPNDDKRVKDAK"
     gene            complement(6220..7881)
                     /locus_tag="O2701_001781"
     CDS             complement(6220..7881)
                     /locus_tag="O2701_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCZ4293669.1"
                     /translation="MSVTEVIFSFLGGLGIFLYGLKIMGDGLQASAGDRLRDILNKFT
                     SNPVLGVIAGIVVTILIQSSSGTTVITIGLVTAGFMTLKQAIGVIMGANIGTTVTAFI
                     IGIDLGEYAMPILALGAFLIFFFKRSKINNIGRILFGFGSLFFGLEFMGDAVKPLASL
                     DGFKQLMLDMSTNPILAVIVGAGLTALVQSSSATIGILQEFYQQDLISLNAAIPVLLG
                     DNIGTTITAILASLAGSIAAKRAALVHVIFNLIGVIIFTIFLPVVIHLISLLQDLWHL
                     KPAMTIAVSHGIFNITNTLIQLPFVAGLAWIVTKLVPGKDIADDYKPQHLNKDLVYHA
                     PGVALQETQKELQNVGQIVLSMFEDIREITKDDKKLIKKLEQKHQAVETINDSIRNYL
                     VRISTKAITKADVERLAVMFDVNRSILKVAELTEEYVAQLKRQHDEDIRITEDAQRGM
                     DKLFNHVAESFDKAIDMLDVYDKTKKDEIVERSRESFNIEHKLRKGHIKRLNRGECTT
                     KGGLLYIDMIGVLERIGYHSRNVSEALVGLNDDVPTDEEIATTEI"
     gene            complement(8207..9982)
                     /locus_tag="O2701_001782"
     CDS             complement(8207..9982)
                     /locus_tag="O2701_001782"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HCZ4293670.1"
                     /translation="MYYSYGNYEAFARPKKPENVENKSAYLIGSGLASLAAACFLIRD
                     GQMEGSKIHILEELPKAGGSLDGENMPLKGYVVRGGREMENHFECLWDLFRSIPSLEI
                     DNASVLDEFYWLNKEDPNYSRCRVIEKQGQRLVTDGDFTLTKTAIKEILDLCLTNEED
                     LDDVKITDVFSDDFFNSNFWIYWKTMFAFEPWHSAMEMRRYLMRFVHHISGLADFSAL
                     KFTKYNQYESLVLPMVEYLKSHGVQFEYDVKVEDIKIDVTTSQKIAREILIDRNGNAE
                     SIKLTINDLVFVTNGSITESSTYGDNDTPAPPTDELGGSWTLWKNLARQSPEFGNPDK
                     FCQNIPKKSWFVSATSTTNNKEIIDTIESICKRDPLAGKTVTGGIITINDSAWQMSFT
                     INRQQQFKDQPENEISTWIYALYSDVNGDYIKKPITECSGNEICQEWLYHLGVSTDKI
                     EDLAKHASNTIPVYMPYITSYFMTRAIGDRPLVVPHQSQNLAFIGNFAETERDTVFTT
                     EYSVRTAMEAVYQLLNIDRGIPEVINSPFDLRVLMDAIYELNDHQDLREITKDSKMQK
                     LALAGFLKKIKGTYIESLLKEHKLL"
     gene            complement(10156..11028)
                     /locus_tag="O2701_001783"
     CDS             complement(10156..11028)
                     /locus_tag="O2701_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCZ4293671.1"
                     /translation="MNKLQDTTFLSYLFTIILWGSAFPMIKIALNDFSAESLSAFRLI
                     LATIILLPFVIIKKLPTPELRDIPVIFILGFCGFVIYHTALNFGEALISAGISGILVS
                     TTPIFSSALAYIFLKEHFSKWNWLSSLVAFIGISIISISKDDYTTINVLGVFIILLAS
                     FSESLYFTFQKKYIEKYGFIAFTLYTIMASSPFMLIFIPEIINDIHGATFTSIVSVLY
                     LAIFPTIIPYVLLAYIVKSVGVSDATMSLYLTPIVSLLLSYLLLDELPTTLAIIGGII
                     TLLGVSLSNFFQNT"
     gene            11141..12538
                     /locus_tag="O2701_001784"
     CDS             11141..12538
                     /locus_tag="O2701_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCZ4293672.1"
                     /translation="MAKYKDIASDIRDKIITGDWFYGMKIPSHRQLAIQYNVNRVTII
                     KSIELLEAEGFIYTKVGSGTYVNDYLNEAHITNKWSEMMLWSSQQRSQYTVQLINKIE
                     TDDSYIHISKGELGISLMPHIQLKKAMSNTASHIEDLSFGYNNGYGYIKLRDIIVERM
                     SKQGINVGRENVMITSGALHAIQLLSIGFLGQDAIIISNTPSYIHSTNVFEQLNFRHI
                     DVPYNQINEIDTIIDRFINFKNKAIYIEPRFNNPTGRSLTNEQKKNIITYSERHNIPI
                     IEDDIFRDIFFSDPTPSIKTYDKLGKVIHISSFSKTIAPAIRIGWIVASEKIIEQLAD
                     VRMQIDYGSSILSQMVVYEMLKNKSYDKHLVKLRYVLKDKRDFMLNILNNLFKDIAHW
                     EVPSGGYFVWLVFKIDIDIKYLFYELLSKEKILINPGYIYGSKEKSIRLSFAFESNEN
                     IKHALYKIYTYVKKV"
     gene            12728..13201
                     /locus_tag="O2701_001785"
     CDS             12728..13201
                     /locus_tag="O2701_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HCZ4293673.1"
                     /translation="MSKIRSFTILSLLIYLAMMCYTVVTYPKLPTKVPIHYNLAGDAD
                     NFADKWVLLLINSAFIVIWLIFFIAGRYYERFAKWSHYNHTPREIRAIKLFLSTLNLE
                     IMSYMSIFTVLEIWQIQHHHQFNLLWFNMIFIIIIGLTLVIFCLLPTIHKMRDSQ"
     gene            13463..15055
                     /locus_tag="O2701_001786"
     CDS             13463..15055
                     /locus_tag="O2701_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCZ4293674.1"
                     /translation="MTLLTVNPFDNVGLSALVAAVPIILFLLCLTVFKMKGIYAALTT
                     LVVTLIVALFVFELPARVSAGAITEGVVAGIFPIGYIVLMAVWLYKVSIKTGQFSIIQ
                     DSIASISVDQRIQLLLIGFCFNAFLEGAAGFGVPIAICAVLLIQLGFEPLKAAMLCLI
                     ANGAAGAFGAIGLPVSIIDTFNLSGGVTTLDVARYSALTLPILNFIIPFVLVFIVDGM
                     KGIKEILPVILTVSGTYTGLQLLLTIFHGPELADIIPSLATMVVLAFVCRKFKPKNIF
                     RLEASEHKIQKRTPKEIVFAWSPFVILTAFVLVWSAPFFKKLFQPGGALESLVIKLPI
                     PNTVSDLSPKGIALRLDLIGATGTAILLTVIITILITKLKWKSAGALLVEAIKELWLP
                     ILTISAILAIAKVMTYGGLTVAIGQGIAKAGAIFPLFSPVLGWIGVFMTGSVVNNNTL
                     FAPIQATVAQQISTSGSLLVAANTAGGVAAKLISPQSIAIATAAVKKVGEESALLKMT
                     LKYSIIFVAFICVWTFILTLIF"
     gene            complement(15384..16910)
                     /gene="spa"
                     /locus_tag="O2701_001787"
     CDS             complement(15384..16910)
                     /gene="spa"
                     /locus_tag="O2701_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal protein A"
                     /protein_id="HCZ4293675.1"
                     /translation="MKKKNIYSIRKLGVGIASVTLGTLLISGGVTPAANAAQHDEAQQ
                     NAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAPKADAQQNNFNK
                     DQQSAFYEILNMPNLNEAQRNGFIQSLKDDPSQSTNVLGEAKKLNESQAPKADNNFNK
                     EQQNAFYEILNMPNLNEEQRNGFIQSLKDDPSQSANLLSEAKKLNESQAPKADNKFNK
                     EQQNAFYEILHLPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNDAQAPKADNKFNK
                     EQQNAFYEILHLPNLTEEQRNGFIQSLKDDPSVSKEILAEAKKLNDAQAPKEEDNNKP
                     GKEDNNKPGKEDNNKPGKEDNNKPGKEDGNKPGKEDNKKPGKEDGNKPGKEDNKKPGK
                     EDGNKPGKEDGNKPGKEDGNGVHVVKPGDTVNDIAKANGTTADKIAADNKLADKNMIK
                     PGQELVVDKKQPANHADANKAQALPETGEENPFIGTTVFGGLSLALGAALLAGRRREL
                     "
     gene            complement(17331..18083)
                     /gene="sarS"
                     /locus_tag="O2701_001788"
     CDS             complement(17331..18083)
                     /gene="sarS"
                     /locus_tag="O2701_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarS"
                     /protein_id="HCZ4293676.1"
                     /translation="MKYNNHDKIRDFIIIEAYMFRFKKKVKPEVDMTIKEFILLTYLF
                     HQQENTLPFKKIVSDLCYKQSDLVQHIKVLVKHSYISKVRSKIDERNTYISISEEQRE
                     KIAERVTLFDQIIKQFNLADQSESQMIPKDSKEFLNLMMYTMYFKNIIKKHLTLSFVE
                     FTILAIITSQNKNIVLLKDLIETIHHKYPQTVRALNNLKKQGYLIKERSTEDERKILI
                     HMDDAQQDHAEQLLAQVNQLLADKDHLHLVFE"
BASE COUNT         5971 a         2754 c         3244 g         6182 t
ORIGIN      
        1 aaataaagaa aagttttgag aagacattga gtatgtatcc gattaaaaat ctagaggatt
       61 tatacgataa agaaggttat cgtgatgacg aatttgataa aaatgataaa ggtacatgga
      121 ttattggttc tgaaatggtt gttcaaccta aaggggaacg tatgaaatca aaaggtatgg
      181 ttctatatat gaatagaaac actaagacta caaccgggaa gtatatagtg agtgaaacac
      241 ttcatgatga agatggtaga cctaagagta aagataaaga atatcccgtt aaaatggtag
      301 acaataaaat cattccaact aaaggtatca aagacgaaaa tataaaaaaa gaaatcgaga
      361 attttaagtt cttcgcgcaa tatggcagct ttaaagattt gtcgaagtac aaagacggcg
      421 atatatcata taatccagag gtaccgagtt attcagcaaa atatcaatta actaatgatg
      481 actataatgt aaaacaatta cgaaaaagat ataaaatacc aacgaataaa gcaccaaagt
      541 tattgttgaa aggttcggga gacttaaaag gttcatcagt tggatataaa gacattgaat
      601 ttacttttgt ggagaaaaaa ggtgaaaata ctttttttac tgatagtcta catcttgaac
      661 cgagtgagga taaataatca tccacacaca cgattcaata tgaaattcaa atatgttgct
      721 gtaaaataat gtaaaataaa cgtattcata ttactgaatt gagggatagt atgcaacgtg
      781 attatttaat tcgagtagaa actgagagta tgtcagattt caaaaggctc aatagtttaa
      841 tgattggttt tgttattaaa ggtgaggcac acatttatga tgaaaataat atgacgcaat
      901 gcaacagtgg cgacattttc atcattaacc accgcgactt gtatcgattt caacttcaac
      961 aagatggcat catatgttat atccaattcc aaatgaaata tttagcagac aagtttgatg
     1021 atgtgcattg tctatatttt cacttaacag atgcgaccac aacaaagaat atacatcaac
     1081 tgagaaatat aatggcaaga ctggtttcaa cacatatccg acataatgag ttgtctaaat
     1141 tgactgagca acaacttgtg attcagttgc ttatgcatat gattcattat gtcccgcgta
     1201 catatcattc gaaccaaagt atcttaaatg atgataaagt gaatcaagta tgcgactata
     1261 tcgagttaca ttttcatgaa gatttaagcc tttcagaatt aagcgaatac gttgggtggt
     1321 cagagagcca tctgtctaaa aagtttacag aatcgctagg tgtaggattc catcatttct
     1381 taaatacgac gcgaattgag catgcgaaac tcgatttgac atacacagat gaaaccatta
     1441 ctgatattgc attgcaaaat ggcttttcaa gtgcagcgag ctttgcgaga acatttaaac
     1501 actttacgca tcaaacacct aaacaatatc gaggtgatcg tccagcaatc actgaaaacc
     1561 agcaatcggc acaacatgat tatcacgacc gtgaattgat attactttta aatgactaca
     1621 ttgaagaaat gaatcatttt attgaagata ttgaaaagat gaactataaa gagattgcct
     1681 ttcaaccaac caatcaccaa ctaaatcaat ttaatcatat tattcaagtg ggctatttga
     1741 ggaatttgct caatacacag tatcaatcac agttgcttac atgtcatcat gattttcaag
     1801 tcaatgaagt attagcatat gatgtgattc catatatcat gaaaaagctc aatgcgccat
     1861 tcacgtatga tgcagaaatt tcgaatatat tttatgatat tgatttgtgt ttagactttt
     1921 tattagatca taactttagt ttgaccatgc atttggatca gtatgactca cgtgattata
     1981 tcgatgcatt taaaattttt atccatcacg ttgccctgca tgtcagtcat agaaaagatt
     2041 tgaagttcaa cttgtatgtg acgacattgc acacgtcttt gattgaaatg attgattatt
     2101 ttaaagcatt attccctaat ggtggcttgt acattcactt agatcaagct acggaaagac
     2161 atctaccatt attgaaacga cttgagccac acatcgacca ttttgtattt gatgccaatt
     2221 caaatgacgc tgttgatttt aataaaatga atgatgatga atttaaaacc gcgagtcaaa
     2281 tgattattaa taaaacgaat taccttatcg acttaatgca tcgtcatcgc ctaaagcgtc
     2341 cactcatttt actcaattgg aatacattga cgggtgatac atttattaca aacggcgaat
     2401 attttagagg tggtatcatc attgagcaat tattaaaatt aagttctaaa gtagagggta
     2461 tcggttattg gttgaattat gatttgcacg tcagtcattg taaaaatgaa cgggattata
     2521 tgaattctat tgaactattt catcaatata atggaaaacg tccggtctat ttcacggcat
     2581 tgctatttaa taaattaaca agcaatattt tgtattctga tgatacatgt attgtcacgg
     2641 ggactgattc aaattttcaa atattgttat atgatgcaaa gcattttaat ccgtacttag
     2701 cgttggacaa tcaaatgaat atgcgtgcga cggaaatgat tcatttgaac attaatgccc
     2761 tggaagaagg tatgtataag attaaacatt ttaccttaga taaagaaaat ggtgcgttat
     2821 ttaatctttg gcgcaaacat catacgatac atggcatgga caaggactct atagattacg
     2881 ttaatcgaat gagttttccg aaattagaag tatatgatat agatatcacg gacacactgg
     2941 cattaaacat taaaatgatt acgaatggga ttcacttaat tgaagtaaaa cgttacccaa
     3001 gttcataaaa tgatcacaaa tcacaaattt tgatatacat aatttgtgat tttttatatt
     3061 caaagctaaa attgcaaaaa attaatggtt aacatctctg ttgttggcaa tataaataat
     3121 gattaatcat ttatgatgta actaaggaga tgaaggatat gaatcaacaa ttaattgaaa
     3181 ctttaaaatc taaagaaggc aaaatgattg agatcagacg ttatttacat cagcatccag
     3241 aattatcttt tcatgaagat gaaacggcga aatacatcgc tgaattttac aaaggtaaag
     3301 atgtggaagt agaaacgaat gtcggaccac gtggaattaa agtaacgatt gattcaggga
     3361 aacctggtaa aacattagca atccgtgcag actttgacgc attacccatt actgaagata
     3421 caggattatc ttttgcatca caaaataaag gtgttatgca cgcatgtggt cacgatgcac
     3481 atacagcata catgcttgta ttagcagaga cgcttgctga aatgaaagat agttttacag
     3541 gaaaagtcgt tgtgatacat caaccagctg aagaagtacc accaggtggt gctaaaacaa
     3601 tgattgaaaa tggtgtatta gacggtgttg atcatgtatt aggtgtacac gtcatgagca
     3661 caatgaaaac aggtaaagtg tattacagac ctggttatgt tcaaacagga cgcgcattct
     3721 tcaaattgaa agttcaaggt aaaggtggtc atggttcatc accacatatg gccaatgatg
     3781 ccattgttgc aggtagctac ttcgtcacag cgttacaaac agttgtatct agacgactaa
     3841 gtccatttga aaccggtgtt gtcacaatcg gttcatttga cggtaaaggt caattcaatg
     3901 tcattaaaga tgttgttgaa attgaaggtg atgtacgtgg attaacagat gctacaaaag
     3961 caacaattga aaaagaaatt aaacgtttat caaaaggatt agaggatatg tatggtgtaa
     4021 cttgcacctt agaatataac gatgattatc cagcattata taatgatcca gagtttactg
     4081 agtacgtggc taagacgttg aaagaagcaa accttgattt tggtgttgaa atgtgtgaac
     4141 cacaaccacc ttcagaagac tttgcatatt atgctaaaga acgcccaagt gcctttattt
     4201 atacaggtgc agcggtggaa aatggtgaaa tttacccaca tcatcatcct aaatttaaca
     4261 tttcagaaaa atcattactt atttctgcag aagcagtagg gacagttgtt ttagattacc
     4321 ttaaaggaga taactaacat gaatgaaacg tatcgcgggg gcaacaagtt aatcttaggt
     4381 attgtattag gtgttattac attttggttg tttgcacaat cacttgtaaa tgttgtaccg
     4441 aatttacaac aaagttttgg tacagacatg gggacaatta gtattgcggt cagtctaact
     4501 gcactatttt caggcatgtt tgttgttgga gcaggtggtc tggcagataa aattgggcgc
     4561 gtgaaaatga cgaatatcgg tttattatta agtattattg gttcagcatt aattattatt
     4621 acgaatttac cggcattatt aattttaggt cgtgttatac aaggtgtatc agcagcgtgt
     4681 attatgcctt ctacattggc cattatgaaa acttattatc agggtgctga acgtcagcgt
     4741 gccttaagtt attggtctat cggttcttgg ggtggcagtg gtatctgttc actcttcggt
     4801 ggtgcagttg cgacaactat gggttggaga tggattttca tcttctcaat tatcgttgcc
     4861 gtactttcaa tgttactcat caaagggacg cctgaaacga aatcagaaat taccaataca
     4921 cataaatttg acgttgcagg gctaattgtt ctagtagtta tgttgctaag tttaaacgtt
     4981 gtcattacta aaggtgcagc acttggttac acatcattat ggttctttgg tttgattgca
     5041 atcgtaattg tagcattctt tattttctta aatgttgaga aaaaagtaga taatccactt
     5101 attgatttta aattatttga aaataaacca tatacaggtg caacgatttc gaacttctta
     5161 ttaaacggtg ttgcaggtac attaattgta gcgaatacat tcgtgcaaca aggtttaggt
     5221 tatacagcat tgcaggcagg atacttatca attacttatt taatcatggt gttattgatg
     5281 attcgagttg gtgaaaaatt attacaaaaa atgggttcta agcgaccaat gttattaggt
     5341 acattcattg tggtcattgg tattgcactt atttcattag tattcttacc aggcatattt
     5401 tatgttatca gttgtgtcgt aggatattta tgtttcggac taggcttagg tatttatgca
     5461 acaccttcta cagatacagc tatttcgaat gcaccgttag ataaagttgg cgttgcttca
     5521 ggtatttata aaatggcttc atcacttggt ggcgcattcg gtgtcgcaat tagtggtgct
     5581 gtatatgctg gtgcagttgc tgcaacgagc attcatacag gtgcgatgat tgcactttgg
     5641 gttaacgtat taatgggaat catggcattt atcgcaattt tattcgcgat tcctaatgat
     5701 gataaacgtg tcaaagatgc gaaataataa cgatgacacg ccaagtgttt tgataatgat
     5761 ataaatgaaa gtaaagcaaa ttaaatgagg aaatgtatag agcagtgcat atagataaat
     5821 ctatgattgg attaacacta atataaatat aaatgaaaat gaaaaaccat ccacttaaat
     5881 ttttgataag ttaggtggat ggttatttat tgcgttgttc taaaaatttg gacatttata
     5941 tggaaagaac tatatataat gtgaaggtta tggcaattcg cttcaatgaa ggcgacgtgt
     6001 aaaagtgtat attaaacgtt tcggggtcag actccgagaa cagtgtccgt catgcttacc
     6061 gcatactaat gaagcattat tatatcagtg tttatagccg atttaagata ataaattttg
     6121 ctaaacaaaa aagccaaccc atgaatgttg gattggcttt ttacatgcat ctgaatctct
     6181 aattttaaaa aaatatgaat ataaataaga cagtaaaaat taaatttcag ttgttgcaat
     6241 ttcttcatct gtaggtacat catcgttaag gccaacaagt gcttcagaaa catttcgtga
     6301 atgataaccg atacgttcaa gaacaccaat catatcgata tatagtaatc cgccttttgt
     6361 tgtacattca ccacgattaa ggcgtttaat atgacctttg cgtagtttat gttcaatatt
     6421 aaatgattct ctactacgtt ctacaatttc atcttttttc gttttgtcat aaacatctaa
     6481 catgtcgatg gctttatcaa atgactcagc aacatggttg aataatttat ccataccgcg
     6541 ttgtgcatct tctgtaatgc gaatatcttc atcatgttgg cgttttaatt gagcgacata
     6601 ctcttctgtt agctctgcta cttttaaaat agagcgattg acatcaaaca taactgctaa
     6661 acgctcaacg tctgccttcg taatggcttt tgtagaaatt ctaactaaat aatttcgaat
     6721 gctatcattg attgtttcaa cagcttgatg cttttgttca agctttttga tcaatttttt
     6781 atcgtctttt gtaatttcgc gaatgtcttc aaacattgat aagacaatct gacccacatt
     6841 ttgtaattct ttttgagttt cttgtaatgc aacaccaggt gcgtgataaa caagatcttt
     6901 gtttaagtgc tgaggtttat agtcatcagc aatatcttta cctgggacaa gctttgtaac
     6961 aatccatgct aaacctgcta caaatggtaa ttgaatcaaa gtatttgtta tgttgaagat
     7021 accatgtgat actgcaatcg tcatcgctgg ttttaagtgc cataaatctt gtaacaaact
     7081 aatcaaatga atcacaactg gcaagaaaat tgtgaagata attaccccga ttaagttaaa
     7141 gatgacgtgt acaagcgccg cacgttttgc agcgattgag ccggctaaac tagctaagat
     7201 agctgtaatc gtggtaccaa tgttatcgcc tagtaacaca gggattgctg cgtttaagct
     7261 aattaaatct tgttgataaa attcttgtaa aataccaatc gtcgcacttg aactttgaac
     7321 tagtgctgtt aaccctgcgc cgacaatgac agcgagtatt ggatttgtag acatatcaag
     7381 cattaattgc ttaaatccat ctaatgatgc taaaggttta acggcatcac ccataaattc
     7441 tagaccgaag aatagtgaac cgaaaccgaa tagtatgcgg ccaatgttat tgattttaga
     7501 gcgtttaaag aaaaagatta agaatgcacc taatgctaaa attggcattg catattcgcc
     7561 taaatctata ccgataataa atgcagttac cgttgttccg atattagcac ccattatcac
     7621 tccaatggct tgtttcaatg tcataaatcc agctgttacc agtccgattg tgataactgt
     7681 cgtacctgaa ctactttgta ttaaaatagt tacaacgata cctgcaataa cacctaatac
     7741 tggatttgat gtaaatttgt ttaaaatatc tcgtagcctg tctcctgctg atgcttgaag
     7801 cccgtctccc atgattttta agccgtaaag gaaaatacct aaaccaccta aaaaggagaa
     7861 aatgacttct gtaaccgaca tttccattat ttcacctcaa ataagcttta tatttagatt
     7921 atcgcttata attgtaaatt taatgttaag attaggtaaa attatttaac aatatatgtt
     7981 atttgtatat gacttgtaaa atatcgtcac ttattatgta aattttcagt gtgaaatggc
     8041 aggtttgcaa tcacttgttt aacaaaatga tgcaatcaat catgtaatta tgtttcatca
     8101 aaaaaatcat gagagtggaa caacgaaata atttttatga aaacatcatt tctgtcccag
     8161 tctcatgatt tgaaatcacc aaataaaaat ctattaatgg ttttcgttat aacaatttgt
     8221 gttcttttaa taatgactca atgtacgtac cttttatctt tttaaggaat cctgctaatg
     8281 cgagtttttg cattttcgaa tctttagtaa tctcacgcaa atcttggtgg tcattcagtt
     8341 cgtatatggc atccattaag acgcgaagat caaatggact attgatgact tctggaatac
     8401 cacgatctat atttagtaat tgataaacag cttccatggc agtacgaacc gaatattctg
     8461 ttgtaaatac agtgtctcgc tctgtttctg caaagttacc aataaatgct aagttctgag
     8521 attgatgcgg gacgactaaa ggtctgtcgc cgatagcacg cgtcatgaaa taagatgtga
     8581 tatatggcat ataaacagga atcgtattag atgcatgttt tgctaagtct tcaattttgt
     8641 cagttgatac acctaagtga tacagccatt cttggcatat ttcattacca ctacattctg
     8701 taattggctt tttaatataa tcgccgttta catctgaata taaggcataa atccatgtag
     8761 atatttcatt ttcaggttgg tctttaaact gttgctgacg attgattgta aaactcattt
     8821 gccatgcaga atcattgatt gtaataatac cgcctgtaac tgttttgcct gcaagtgggt
     8881 cacgtttaca aatactttct attgtatcga taatctcttt attgtttgtt gtagaagttg
     8941 ctgaaacaaa ccaacttttt ttaggaatat tttggcaaaa cttatcagga ttaccaaatt
     9001 caggactttg tcgcgctaaa tttttccata gtgtccaact accacctaat tcgtcagttg
     9061 gtggcgctgg tgtatcatta tcaccataag tagagctttc tgtaatacta ccgtttgtca
     9121 caaagacaag atcgtttata gtcagtttaa tagattctgc attaccatta cggtcaatta
     9181 atatttctcg ggcaattttt tgacttgtcg taacatctat tttaatatct tcgactttta
     9241 catcgtattc aaattgaacc ccatgcgatt ttaaatattc aaccataggt aatactaaag
     9301 attcatattg attatattta gtgaatttta aagctgaaaa gtctgcgaga ccactaatat
     9361 gatgaacgaa tcgcattaga tagcgacgca tttccattgc agaatgccac ggttcaaatg
     9421 caaacatcgt tttccagtaa atccaaaagt ttgaattaaa gaagtcatcg gaaaatacat
     9481 ctgttatttt gacatcatct aaatcttctt cattcgttaa gcataaatct aaaatttctt
     9541 taatcgccgt tttagtcaaa gtgaagtctc cgtctgtgac taaacgttga ccctgtttct
     9601 caataacacg acagcgagaa tagttagggt cttctttgtt tagccaatag aactcatcta
     9661 atacagacgc gttatcgatt tctaatgaag ggatagatct gaataagtcc cacaaacatt
     9721 caaagtggtt ctccatttca cgaccaccgc ggacaacata gccttttaaa ggcatatttt
     9781 caccatcaag actaccacct gctttaggta actcttctaa aatatgaatc ttcgaacctt
     9841 ccatttgacc atcccttatt aaaaaacaag ctgcagcaag tgaagctaga ccagatccga
     9901 ttaagtaagc ggatttgttt tctacatttt caggtttttt agggcgcgca aatgcttcat
     9961 aatttccata actgtaatac atatccacca agtcctttcg tattagaata cactcagact
    10021 ataccccttt gtgaattgca aatattagta tttatattgg taaatttgac gaaattagat
    10081 catttttata tttttgattt ttaaaatttt atcactcgaa tgtttttgga ttctgaggtg
    10141 cgggacttgg aataattatg tattttgaaa gaagttactt aaactaacgc ctagtagagt
    10201 gataattccg cctataatag caagggttgt tggtagctcg tctaataaca gataagataa
    10261 taataaagaa acgataggtg ttaaataaag agacattgtt gcatcagaga caccaactga
    10321 cttcacaata taagcaagca aaacgtatgg aattatagta gggaatatag ctaaataaag
    10381 taccgatact attgatgtaa aagtggcgcc gtgtatatcg ttgatgattt caggaataaa
    10441 aataagcata aatggtgagc ttgccattat tgtatatagt gtgaaagcga tgaagccgta
    10501 tttttctatg tattttttct ggaaagtaaa atacaaactt tcactaaaag atgcaagtaa
    10561 aataataaaa acacctaata cattaatagt tgtgtaatca tctttactta ttgaaataat
    10621 ggatattcct ataaatgcga cgagtgaact taaccaattc cattttgaaa aatgctcttt
    10681 taaaaatata taagctaaag cactagaaaa aataggcgtt gtagagacta gaattccaga
    10741 tatacctgca ctaatcaaag cttcaccaaa atttaaagct gtgtgatata tcacaaatcc
    10801 acaaaatcct aaaataaaaa taacagggat atctcttagt tcaggggtag gcaatttctt
    10861 tataattacg aacggcaaga gaattattgt tgctaaaatt aaacgaaatg ccgacaatga
    10921 ttctgcacta aaatcattta acgcaatctt tatcattgga aatgcagatc cccacaatat
    10981 gatagtaaat aaatatgata gaaaagtagt gtcttgaagt ttattcatta atatcatcac
    11041 tcctttaatt atgtgtttct atattaaaaa atatgattta aaatgagtac aaccaatttt
    11101 gaatggattt acctatccaa ttttaaaagg gagggagaag atggctaaat ataaagatat
    11161 tgctagtgac ataagagata aaataatcac aggggattgg ttttatggaa tgaaaatacc
    11221 ttcacatagg cagttggcga tacagtacaa cgtaaataga gtaacgatta ttaaaagtat
    11281 tgagttatta gaagctgaag gatttatcta tactaaagta ggtagtggaa catatgttaa
    11341 tgactatttg aatgaagcac atattacaaa taagtggtct gaaatgatgt tatggtcctc
    11401 tcaacaaaga agtcagtata cggtgcaatt aattaataaa attgagacag atgattcgta
    11461 tatacatata agtaaaggtg aattgggtat atcgttaatg ccacatattc aattgaaaaa
    11521 agccatgtct aatacagcca gtcatattga agacttatct tttggttata ataatggcta
    11581 tggttatatc aagttaagag atattatcgt tgaacgaatg tcaaagcaag gtataaatgt
    11641 aggtagagaa aatgtaatga tcacttcagg cgctttacat gccattcaac ttttatctat
    11701 tgggttttta ggtcaagatg ccataataat ttcgaataca ccatcatata ttcactctac
    11761 aaatgttttt gagcaattga attttagaca tattgatgtt ccttataatc aaattaatga
    11821 aattgatacc atcattgata gatttattaa ttttaaaaat aaagcgattt atatagaacc
    11881 taggtttaat aacccgacag gtcgttcttt aacgaatgag caaaagaaaa atataattac
    11941 ttatagcgaa agacataata ttcctatcat tgaagatgat atctttagag atattttctt
    12001 tagcgatcca actccttcta tcaaaactta tgataaattg ggaaaagtta tacatataag
    12061 cagtttttca aaaacgattg caccagcaat aagaataggt tggattgttg cttctgaaaa
    12121 aataatagag caattggcag atgtaagaat gcaaattgac tatggatcca gtattttgtc
    12181 acaaatggtt gtatatgaga tgttgaaaaa taagtcttat gataaacact tagtaaagtt
    12241 aaggtatgtt ttaaaagata aacgagactt tatgttaaac atcctcaata atttatttaa
    12301 ggatatagca cattgggagg ttccaagtgg aggttatttt gtatggttag tctttaaaat
    12361 agatatagat attaaatatt tattttacga attgttaagt aaagaaaaaa tattaatcaa
    12421 tccgggttac atttatggca gtaaagaaaa gagtataagg ctatcttttg cctttgaatc
    12481 aaatgaaaat attaagcatg cgctctataa aatttataca tatgtgaaaa aggtttaatt
    12541 aaaacaataa ttcgaatcat tatgtggcat gttaaacagc taaatataag ctatgcacat
    12601 ttaacaagcg gatactcctg acgcaattat agtatcatta catattgttt aatcgtttgg
    12661 ggtaatgtaa ttaaaaggta aaataggcag agtgttatac ataaaatgta aagaaagagg
    12721 cgatagaatg tctaaaatca ggtcttttac aatattaagt ctacttattt acttagctat
    12781 gatgtgctat acagtagtga cctatccaaa attaccaacc aaagtgccta ttcattataa
    12841 tttagcaggg gatgctgata attttgctga taaatgggtg ctgcttttga ttaatagcgc
    12901 atttatagtg atttggctta tatttttcat tgcaggtaga tactatgaac gatttgccaa
    12961 atggtcacat tataatcata caccacgtga aattcgagcg attaaattat ttttaagtac
    13021 gttaaattta gagattatga gctatatgtc tatcttcaca gtattagaaa tttggcaaat
    13081 acaacaccat catcaattca atttactatg gtttaatatg atatttatca ttatcattgg
    13141 tttgacgctt gtcatatttt gtttacttcc tacaattcat aaaatgagag attctcaata
    13201 aaaatataaa ctctcatgat gcagttttga agcgaattcc tgagattatt ataaataata
    13261 tctagtattt ataattaaaa tgatatatta tttattatag ttaaatattg tgtatatttt
    13321 gtgaactttt tgtaaaaatt cgattgcctg tcacatatag gagtgttaca ttttaaatat
    13381 gtgatcatcg caaaatataa gttgaaatag gttgtagatt aatcagaatg ataaatattt
    13441 tatataaaga gagggagtca ttatgacact acttactgta aatccattcg ataatgtcgg
    13501 attatcagcc ttagttgcag cagtacctat tattttattt ttattatgct taaccgtttt
    13561 taaaatgaaa ggcatttatg cagcattgac aactttggtt gttacattga ttgtggcttt
    13621 atttgtattt gaattaccag cgcgtgtatc agcaggtgcg attacagaag gcgttgttgc
    13681 cggtattttc ccaataggat atatcgtttt aatggcagtt tggttatata aagtttctat
    13741 taaaacagga caattttcta ttattcaaga tagtattgca agtatttcag tggaccaaag
    13801 aatccaacta ttattaattg gattttgttt caacgcattt ttagaaggtg cagcaggatt
    13861 tggtgtgcca attgcgattt gtgcagtatt attaattcaa cttggatttg aaccattaaa
    13921 agcagcgatg ttatgtttaa ttgctaatgg tgcggcgggt gcctttggtg caattggttt
    13981 accagttagt attattgata cgtttaactt aagtggaggc gttacaacat tagatgttgc
    14041 gagatactca gcattaacac ttccaatttt aaactttatt attccatttg ttttagtatt
    14101 cattgtagat ggtatgaaag gtattaaaga aattttacct gtcattttaa cagtgagtgg
    14161 tacatatact ggattacaat tattattaac aatattccat ggtccagaac tagcagacat
    14221 tattccatca ctagcaacaa tggtggtgtt agcatttgtt tgtcgtaaat ttaaaccgaa
    14281 aaacattttc agattggaag cgtctgaaca taaaattcaa aaacgaacgc ctaaagaaat
    14341 tgtctttgct tggagtccgt tcgtaatttt aactgccttt gtattagtat ggagtgcacc
    14401 attcttcaaa aaattattcc aacctggagg tgcacttgaa agtttagtaa taaaattgcc
    14461 aattccaaat actgtgagtg atttatcgcc taaaggaatt gcgttgcgtc tcgatttaat
    14521 tggtgcaact gggacagcga ttttattaac agtaattatt acaattttaa ttacgaagtt
    14581 aaaatggaaa agtgcaggtg ctttattggt cgaagcaatt aaagaattat ggttaccgat
    14641 ccttacaatt tcagctatcc tagctattgc taaagttatg acatacggtg gtttgactgt
    14701 agcaattgga caaggtattg ctaaagcggg agcaattttc ccattattct ctccagtatt
    14761 aggttggatt ggtgtgttta tgactggttc agttgtaaat aacaatactt tattcgcacc
    14821 tattcaagcg acagtggcac aacaaatttc aacaagcggt tcattacttg tggcagctaa
    14881 cactgcaggt ggtgtagcag cgaaacttat ttcaccacaa tcaattgcca ttgcgactgc
    14941 agctgttaaa aaagttggtg aagaatctgc attattaaaa atgacgttaa aatacagtat
    15001 tatatttgtt gcttttattt gtgtttggac gtttatacta acgttaatat tctaaatata
    15061 aataatgttg tcacttggat tcaaatgaca ttttaaatct aattattcat gaatcgaact
    15121 agtacgaaat gcaatgagca tcttgtctag ttcgattttt taatgcctaa aaatgtcata
    15181 tatgtaatca gagtagaaag tgttgaggcg tttcagaagt tgtttagaaa agtaagtaaa
    15241 ataaaaaatg cactgagcaa caaaagatgt tgctcgtgca tttagatgat tcttatcatt
    15301 tcaaataaga atgtgttaat caacgtatat aagttaaaat tggtttggat aaaatgatat
    15361 ctatcgttgt gtattgtttg tttttatagt tcgcgacgac gtccagctaa taacgctgca
    15421 cctaaggcta atgataatcc accaaataca gttgtaccga tgaatggatt ttcttcacca
    15481 gtttctggta atgcttgagc tttgttagca tctgcatggt ttgctggttg cttcttatca
    15541 acaacaagtt cttgaccagg tttgatcatg tttttatcag ctaatttgtt atctgcagca
    15601 attttgtcag cagtagtgcc gtttgctttt gcaatgtcat ttactgtatc accaggttta
    15661 acgacatgta ctccgttacc atcttcttta ccaggtttgt tgccatcttc tttgccaggc
    15721 ttgttgccgt cttctttacc aggttttttg ttgtcttctt taccaggctt gttgccatct
    15781 tctttaccag gttttttgtt gtcttcttta ccaggcttgt tgccgtcttc tttgccaggc
    15841 ttgttgttgt cttctttacc aggcttgttg ttgtcttctt tgccaggctt gttattgtct
    15901 tctttgccag gcttgttatt gtcttcctct tttggtgctt gagcatcgtt tagcttttta
    15961 gcttctgcta aaatttcttt gctcactgaa ggatcgtctt taaggctttg gatgaagccg
    16021 ttacgttgtt cttcagttaa gttaggtaaa tgtaaaattt catagaaagc attttgttgt
    16081 tctttgttga atttgttgtc agcttttggt gcttgagcat catttagctt tttagcttct
    16141 gctaaaaggt tagcgctttg gcttgggtca tcttttaggc tttggatgaa accattgcgt
    16201 tgttcttcgt ttaagttagg taaatgtaag atttcataga aagcattttg ttgttctttg
    16261 ttgaatttgt tatccgcttt cggtgcttga gattcattta actttttagc ttctgacaat
    16321 aggttagcac tttggcttgg gtcatctttt aagctttgga tgaaaccatt gcgttgttct
    16381 tcgtttaagt taggcatatt caagatttca tagaaagcat tttgttgttc tttgttgaaa
    16441 ttgttatcag ctttcggtgc ttgagattcg tttaattttt tagcttcacc taaaacgtta
    16501 gtgctttggc ttgggtcgtc tttaagactt tgaatgaagc cgttacgttg cgcttcgttt
    16561 aagttaggca tgttcaagat ttcatagaag gcgctttgtt gatctttgtt gaagttattt
    16621 tgttgcgcat cagcttttgg agcttgagag tcattaagtt tttgagcttc acctaaaacg
    16681 ttagcacttt ggcttggatc atctttaagg ctttggataa aaccattgcg ttgatcagca
    16741 tttaagttag gcatatttaa gacttgataa aaagcatttt gttgagcttc atcgtgttgc
    16801 gcagcatttg cagcaggtgt tacgccacca gatataagta atgtacctaa agttacagat
    16861 gcaataccta cacctagttt acgaattgaa taaatgtttt tctttttcaa attaataccc
    16921 cctgtatgta tttgtaaagt catcataata taacgaatta tgtattgcaa tactaaaatc
    16981 tatatttata attaaattta aaggtaagtt ttacaactta taaaataaat attttgctga
    17041 aagatttatt caggaagtaa aaggcttaaa accgcaaaat cacgctattt cgattaataa
    17101 aaacagataa aatatagaga tattttttat aatgaaagcg gtttaataca tatgagttaa
    17161 aaaatatttt taagaataaa gtggggcttt gaatgtgctg aggtttaatt tcggaaatgg
    17221 ttttaagtga tatagaaaat agaaagttgt tcattatgtg ctgaggtttt attaaaaaat
    17281 agaaaacaca agtgcacttt agaatacagc acacttgcgt aatagagata ttattcaaaa
    17341 acaagatgta aatgatcttt atctgctaat aattgattca cttgagctaa taattgttca
    17401 gcatggtctt gctgcgcgtc atccatatga attaaaattt ttctttcatc ttcagttgag
    17461 cgttctttta ttagatagcc ttgctttttt aaattattga gagctctaac agtttgaggg
    17521 tatttatggt ggattgtttc aattaaatct ttaagaagaa cgatgttttt attttgagaa
    17581 gtgataatag ctagaattgt gaattctaca aaacttaatg ttagatgttt tttgataata
    17641 ttcttgaaat acattgtata catcatcaag tttagaaatt ctttactatc ttttggtatc
    17701 atctgtgatt cactttgatc tgcaaggtta aattgtttaa tgatttgatc aaacaatgta
    17761 acacgttctg caattttctc tcgttgttct tcagatattg aaatgtaagt attacgctca
    17821 tcaattttac ttcgaacttt actaatatat gaatgtttca caagtacttt tatatgctgt
    17881 actaaatccg attgtttata acataaatct gaaacaatct tcttaaatgg aagtgtgttt
    17941 tcttgttgat gaaataaata agtcagtaat ataaattctt ttatagtcat atcgacttca
    18001 ggcttgactt ttttcttaaa acgaaacata tatgcttcaa tgattataaa atctctaatt
    18061 ttgtcatggt tattatattt cattgtttta tctccttgta tatgcacttt attttaatat
    18121 aacacaatat aataagaaaa ataatccata a
//
LOCUS       DALAGD010000065        18049 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.066, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000065 DALAGD010000000
VERSION     DALAGD010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18049
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.066"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            34..435
                     /locus_tag="O2701_001789"
     CDS             34..435
                     /locus_tag="O2701_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293677.1"
                     /translation="MSVEIKGIPEVLNKLESVYGKQAMQAKSDKALNEASEFFIKALK
                     KEFESFKDTGASIEEMTKSKPYTKVGSQERAVLIEWVGPMNRKNIIHLNEHGYTRDGK
                     KYTPRGFGVIAKTLAASERKYREIIKKELAR"
     gene            436..831
                     /locus_tag="O2701_001790"
     CDS             436..831
                     /locus_tag="O2701_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HCZ4293678.1"
                     /translation="MNILNTIKGILLSDAELKTHINSRIYYYKVTENAETSKPFVVIT
                     PVYDLPSDFMSDKYLSEEYLIQIDVESSNNQKTIDITKRIRYLLYQQNLIQASSQLDA
                     YFEETKRYVMSRRYQGIPKNIYYKNQRIE"
     gene            866..1507
                     /locus_tag="O2701_001791"
     CDS             866..1507
                     /locus_tag="O2701_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCZ4293679.1"
                     /translation="MAEGQGSYKVGFKRLYVGVFNPEATKVVKRMTWEDEKGGTVDLN
                     ITGLAPDLVDMFASNKRVWMKKQGTNEVKSDMSIFNIPSDDLNTVIGRTKDKNGTSWV
                     GENTRAPYVTVIGESEDGLTGQPVYVALLKGTFSLDSIEFKTRGEKAEAPEPTKLTGD
                     WMNRKVDVDGTSQGIVYGYHEGKEGEAEFFKKVFVGYTDSEDHSEDSAGSLPS"
     gene            1599..2054
                     /locus_tag="O2701_001792"
     CDS             1599..2054
                     /locus_tag="O2701_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HCZ4293680.1"
                     /translation="MTKTLKVYKGDDVVASEQGEGKVSVTLSNLEADTTYPKGTYQVA
                     WEENGKESSKVDVPQFKTNPILVSGVSFTPETKSIMVNTDDNVEPNIAPSTATNKILK
                     YTSEHPEFVTVDENTGAIHGVAEGTSVITAMSTDGSDKSGQISVTVTNG"
     gene            2112..2462
                     /locus_tag="O2701_001793"
     CDS             2112..2462
                     /locus_tag="O2701_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293681.1"
                     /translation="MIKFEIKDRKTGKTESYTKEDVTMGEAEKCYEYLELVNQENKKE
                     APNATKMRQKERQLLVDLFKDEGLTEEDVLNKMSTKTYTKALQDIFREINGEDEEDSE
                     TEPEEMGKTEEQSQ"
     gene            2504..2662
                     /locus_tag="O2701_001794"
     CDS             2504..2662
                     /locus_tag="O2701_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293682.1"
                     /translation="MEQYGWTLTEVRKQPYVKLLEILNEENKEETEEKQSEQKVITGT
                     DLRKLFGS"
     gene            2676..8876
                     /locus_tag="O2701_001795"
     CDS             2676..8876
                     /locus_tag="O2701_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCZ4293683.1"
                     /translation="MNEKVEGMTLELKLDHLGVQECMKGLKRQLGVVNSEMKANLSAF
                     DKSEKSMEKYQARIKGLNDRLKVQKKMYSQVEDELKQVNANYQKAKSSVKDVEKAYLK
                     LVEANKKEKLALDKSKEALKSSNTELKKAENQYKRTNQRKQDAYQKLKQLRDAEQKLK
                     NSNQATTAQLKRASDAVQKQSAKHKALVEQYKQEGNQVQKLKVQNDNLSKSNDKIESS
                     YAKTNTKLKQTEKEFNDLNNTIKNHSANVAKAETAVNKEKAALNNLERSIDKASSEMK
                     TFNKEQMIAQSHFGKLASQADVMSKKFSSIGDKMTSLGRTMTMGVSTPITLGLGAALK
                     TSADFEGQMSRVGAIAQASSKDLKSMSNQAVDLGAKTSKSANEVAKGMEELAALGFNA
                     KQTMEAMPGVISAAEASGAEMATTATVMASAINSFGLKASDANHVADLLARSANDSAA
                     DIQYMGDALKYAGTPAKALGVSIEDTSAAIEVLSNSGLEGSQAGTALRASFIRLANPS
                     KNTAKEMKKLGIHLSDAKGQFVGMGELIRQFQDNMKGMTREQKLATVATIVGTEAASG
                     FLALIEAGPDKINSYSKSLKNSNGESKKAADLMKDNLKGALEQLGGAFESLAIEVGKD
                     LTPMIRAGAEGLTKLVDGFTHLPGWVRKASVGLALFGAAIGPAVLAGGLLIRTVGSAA
                     KGYASLNRRIAENTILSNTNSKAMKSLGLQTLFLGSTTGKTSKGFKGLAGAMMFNLKP
                     INVLKNSAKLAILPFKLLKNGLGLAAKSLFAVSGGARFAGVALRFLTGPIGATITAIT
                     IAYKVFKTAYDRVEWFRNGINGLGETIKFFGGKIIGGAVRKLGEFKNYLGSIGKSFKE
                     KFSKDMKDGYKSLSDDDLLKVGVNKFKGFMQTMGTASKKASDTVKVLGKGVSKETEKA
                     LEKYVHYSEENSRIMEKVRLNSGQISEDKAKKLLKIETDLSNNLIAEIEKRNKKELEK
                     TQELIDKYSAFDEQEKQNILTRTKEKNDLRIKKEQELNQKIKELKEKALSDGQISENE
                     RKEIEKLENQRRDITVKELSKTEKEQERILVRMQRNRNAYSIDEASKAIKEAEKARKA
                     RKKEVDKQYEDDVIAIKNNVNLSKSEKDKLLAIADQRHKDEVRKAKSKKDAVVDVVKK
                     QNKDIDKEMDLSSGRVYKNTEKWWNGLKSWWSNFREDQKKKSDKYAKEQEETARRNRE
                     NIKKWFGNAWDGVKSKTGEAFSKMGRNANHFGGEMKKMWSGIKGIPSKLSSGWSSAKS
                     SVGYHTKAIANSTGKWFGKAWQSVKSTTGSIYNQTKQKYSDASDKAWAHSKSIWRGTS
                     KWFSNAYKSAKGWLTDMANKSRAKWDNISSTAWSNAKSVWKGTSKWFSNSYKSLKDWT
                     GDMYSRAHDRFDAISSSAWSNAKSVFNGFRKWLSKTYDWIRDIGKDMGRAAADLGKNV
                     ANKAIGGLNSMIGGINKISKAITDKNLIKPIPTLSTGTLAGKGVATDNSGALTQPTFA
                     VLNDRGSGNAPGGGVQEVIHRADGTFHAPQGRDVVVPLGVGDSVINANDTLKLQRMGV
                     LPKFHGGTKKKDWLDQLKGNIGKKAGEFGATAKNTAHNIKKGAEEMVEAAGDKIKDGA
                     SWLGDKIGDVWDYVQHPGKLVNKVMSGLNINFGGGANATVKIAKGAYSLLKKKLVDKV
                     KSWFEDFGGGGDGSYLFEYPIWQRFGRYTGGLNFNGGRHYGIDFGMPSGTNVYAVKGG
                     IADKVWTDYGGGNSIQIKTGANEWNWYMHLSKQLARQGQRIKAGQLIGKSGATGNFVR
                     GAHLHFQLMQGSHPGNDTAKDPEKWLKSLKGSGVRSGSGVNKAASAWAGDIRRAAKRM
                     GVNVTSGDVGNIISLIQHESGGNAGITQSSALRDINVLQGNPAKGLLQYIPQTFRHYA
                     VRGHNNIYSGYDQLLAFFNNRYWRSQFNPRGGWSPSGPRRYANGGLITKHQLAEVGEG
                     DKQEMVIPLTRRKRAIQLTEQVMRIIGMDGKPNNITVNNDTSTVEKLLKQIVMLSDKG
                     NKLTDALIQTVSSQDNNLGSNDAIRGLEKILSKQSGHRANANNYMGGLTN"
     gene            8876..9700
                     /locus_tag="O2701_001796"
     CDS             8876..9700
                     /locus_tag="O2701_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HCZ4293684.1"
                     /translation="MQSFVKIIDGYKEEVITDFNQLIFLDARAESPNTNDNSVTINGV
                     DGILPGAISFAPFSLVLRFGYDGIDVIDLNLFEHWFRSVFNRRHPYYVITSQMPGVKY
                     AVNTANVTSNLKDGSSTEIEVSLNVYKGYSESVNWTDSEFLFDSNWMFENGIPLDFTP
                     KYTHTSNQFTIWNGSTDTINPRFKHDLKILINLNASGGFELVNYTTGDIFKYNKSIDK
                     NTDFVLDGVYAYRDINRVGIDTNRGIITLAPGKNEFKIKGDVSDIKTTFKFPFIYR"
     gene            9709..11292
                     /locus_tag="O2701_001797"
     CDS             9709..11292
                     /locus_tag="O2701_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCZ4293685.1"
                     /translation="MDYHDHLSVMDFNELICENLLDVDYGSFKEYYELNEARYITFTV
                     YRTTHNSFVFDLLICENFIIYHGEKYTIKQTAPKVEGDKVFIEVTAYHIMYEFQNHSV
                     ESNKLDDDSSETGKTPEYSLDEYLRYGFANQKTSVKMTYKIIGDFKRKVPIDELGNKN
                     GLEYCKEAVDLFGCIIYPNDTEIGFYSPETFYQRSEKVIRYQYNTDTVSATVSTLELR
                     TAIKVFGKKYTAEEKKNYNPIRTTDIKYSNGFIKEGTYRTETIGSKATINFDCKYGNE
                     TVRFTIKKGSQGGIYKLILDGKQIKQISCFAKSVQSETIDLIKNIDKGKHVLEMIFLG
                     EDPKNRIDISSNKKAKPCMYVGTEKSTVLNLIADNSGRNQYKAIVDYVADSAKQFGIR
                     YANTQTNEDIETQDKLLEFAKKQINDTPKTELDVNYIGYEKIEPRDSVFFVHELMGYN
                     TELKVVKLDRSHPFVNAIDEVSFSNEIKDMVQIQQALNRRVIAQDNRYNYQANRINHL
                     YTSTLNSPFETMDIGSVLI"
     gene            11292..11582
                     /locus_tag="O2701_001798"
     CDS             11292..11582
                     /locus_tag="O2701_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293686.1"
                     /translation="MATEEVKIKALLENDKQYFPATHWKAINGIPYAGSSDIDGLPQD
                     GIISVDDKNKLDKLKIGEAGIIQNSIVQKSPNGKLWKITVDDSGKLGTVLFY"
     gene            11598..13508
                     /locus_tag="O2701_001799"
     CDS             11598..13508
                     /locus_tag="O2701_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293687.1"
                     /translation="MENLYLIKDLGALAGRDYRAKEIQNLQRIEQFALGLTTEFKLHQ
                     KAKTMQHFAEQIYYNGRSQAAVNKSLQSQINALVVAPRNNSANEIVQARVNVNGETFD
                     TLKEHLDDWETKTQINKEETIRELNKTKQEILDIEYRFEPDKQEFLFVTELAPLTNAV
                     MQSFWFDNRTGIVYMTQARNNGYMLSRLRPNGQFIDSSLIVGGGHGTHNGYRYIDDEL
                     WIYSFILNGNNENTLVRFKYTPNVEISYGKYGMQDVFTGHPEKPYITPVINEKENKIL
                     YRIERPRSQWELENSMNYIEIRSLDDVDKNIDKVLHKISIPMRLTNETQPMQGVTFDE
                     KYLYWYTGDSNPNNRNYLTAFDLETGEEAYQVNADYGGTLDSFPGEFAEAEGLQIYYD
                     KDSGKKALMLGVTVGGDGNRTHRIFMIGQRGILEILHSRGVPFIMSDTGGRVKPLPMR
                     PDKLKNLGMLTEPGLYYLYTDHTVQIDDFPLPREWRDAGWFLEVKPPQTGGDVIQILT
                     RNSYARNMMTFERVLSGRTGDISDWNYVPKNSGKWERVPSFITKMSDINIVGMSFYLT
                     TDDTKRFTDFPTERKGVAGWNLYVEASNTGGFVHRLVRNSVTASAEILLKNYDSKTSS
                     GPWTLHEGRIIS"
     gene            13508..14974
                     /locus_tag="O2701_001800"
     CDS             13508..14974
                     /locus_tag="O2701_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HCZ4293688.1"
                     /translation="MSNLEKSVAINLENTAHYENISNLDITFRTGESDSSVLLFNIIK
                     NNQPLLLSEENIKARIAIRGKGVMVVAPLEILDPFKGILKFQLPNDVIKRDGSYQAQV
                     SVAELGNSDVVVVERTITFNVEKSLFSKIPSETKLHYIVEFQELEKTIMDRAKAMDEA
                     IKNGEDYASLIEKAKEKGLSDIQIAKSSSIDELKQLANSRISDLENKAQAYSRTFDEQ
                     KRYMDEKHEAFKQSVNSGGLVTSGSTSNWQKAKITKDDGKIMQITGFDFNNPEQRIGD
                     STQFIYVSQAINYPRDVSTNGTVEYLVVTSDYKRMTYRPNGTNKVFVKRKEAGSWSEW
                     SELAINDYNTPFETVQSAQSKANMAESNAKLYADDKFNKRYSVIFDGTANGVGSTLYL
                     NESLDQFILLIFYGTFPGGDFTEFGSPFGGGKISLNPSNLPDGDGNGGGVYEFGLTKS
                     SRTSLTISNDVYFDLGSQRGSGANANRGTINKIIGVRK"
     gene            14974..15363
                     /locus_tag="O2701_001801"
     CDS             14974..15363
                     /locus_tag="O2701_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2977 domain-containing protein"
                     /protein_id="HCZ4293689.1"
                     /translation="MQILVNKRNEIISYAIIGGFEEGIDIENLPENFSQVFRPKAFKY
                     SNGEIVFNEDYSEEKDDLHQQIDSEEQNTVASDDILRKMVASMQKQVVQSTKLSMQVN
                     KQNALMAKQLVTLNKKLEEVKGETENA"
     gene            15356..15520
                     /locus_tag="O2701_001802"
     CDS             15356..15520
                     /locus_tag="O2701_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCZ4293690.1"
                     /translation="MLKLISPTFEDIKTWYQLKEYSKEDIAWYVDMEVIDKEEYAIIT
                     GEKYPENLES"
     gene            15566..15865
                     /locus_tag="O2701_001803"
     CDS             15566..15865
                     /locus_tag="O2701_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 domain-containing protein"
                     /protein_id="HCZ4293691.1"
                     /translation="MFGFTKRHEQDWRLTRLEENDKTMFEKFDRIEDSLRTQEKIYDK
                     LDRNFEELRRDKEEDEKNKEKNAKNIRDIKMWILGLIGTILSTFVIALLKTIFGI"
     gene            16001..16303
                     /locus_tag="O2701_001804"
     CDS             16001..16303
                     /locus_tag="O2701_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCZ4293692.1"
                     /translation="MDAKVITRYIVLILALVNQFLANKGISPIPVDEESVSSIILTVV
                     ALYTTYKDNPTSQEGKWANQKLKKYKAESKYRKATGQAPIKEVMTPTNMNDTNDLG"
     gene            16314..17768
                     /locus_tag="O2701_001805"
     CDS             16314..17768
                     /locus_tag="O2701_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCZ4293693.1"
                     /translation="MLITKNQAEKWFDNSLGKQFNPDLFYGFQCYDYANMFFMLATGE
                     RLQGLYAYNIPFDNKAKIEKYGQIIKNYDSFLPQKLDIVVFPSKYGGGAGHVEIVESA
                     NLNTFTSFGQNWNGKGWTNGVAQPGWGPETVTRHVHYYDNPMYFIRLNFPNNLSVGNK
                     AKGIIKQATTKKEAVIKPKKIMLVAGHGYNDPGAVGNGTNERDFIRKYITPNIAKYLR
                     HAGHEVALYGGSSQSQDMYQDTAYGVNVGNKKDYGLYWVKSQGYDIVLEIHLDAAGES
                     ASGGHVIISSQFNADTIDKSIQDVIKNNLGQIRGVTPRNDLLNVNVSAEININYRLSE
                     LGFITNKNDMDWIKKNYDLYSKLIAGAIHGKPIGGVVASEVKAPVKNEKNPPVPAGYT
                     LDKNNVPYKKEQGNYTVANVKGNNVRDGYSTNSRITGVLPNNTTITYDGAYCINGYRW
                     ITYIANSGQRRYIATGEVDIAGNRISSFGKFSAV"
BASE COUNT         6851 a         2452 c         3715 g         5031 t
ORIGIN      
        1 tattggctat aatacagtgg ttttatcaga aaaatgagtg tagaaattaa agggatacct
       61 gaagtgttga ataaattaga atcggtatac ggtaaacaag caatgcaggc taagagtgat
      121 aaagctttaa atgaagcatc tgaatttttt ataaaggctt taaagaaaga gttcgagagc
      181 tttaaagata cgggtgccag tatagaagaa atgactaaat ctaagcctta tacaaaagtt
      241 ggcagtcaag aaagggctgt tttaattgaa tgggtaggcc ctatgaatcg caaaaacatt
      301 attcacttga atgaacatgg ttatacaaga gatggaaaaa aatatacacc aagaggtttt
      361 ggagttattg caaaaacatt agctgctagc gaacgtaagt atagagaaat tataaaaaag
      421 gagttggcca gataaatgaa tatattaaac accataaaag gaattttatt atctgatgca
      481 gagctcaaaa cacatataaa ttctagaata tactattaca aagtcactga aaacgctgaa
      541 acttccaaac cctttgttgt tattacacct gtttatgatt tgccttcaga ctttatgtct
      601 gataaatatc tcagtgaaga atacttaatt caaatagatg tagaatcttc aaataatcag
      661 aaaacaattg atataacaaa acgaataaga tacctgttat atcaacaaaa tttaattcaa
      721 gcatctagtc agttagatgc ttattttgaa gaaactaaac gttatgtgat gtcgagacga
      781 tatcaaggca tacccaaaaa tatatattat aaaaatcagc gcatcgaata ggtgtgcttt
      841 ttaattttta aggaggaaat aagcaatggc agaaggacaa ggttcttata aagtaggttt
      901 taaaagatta tacgttggag tttttaaccc agaagcaaca aaagtagtta aacgcatgac
      961 atgggaagat gaaaaaggtg gtacagttga cctaaatatc acaggtttag caccagattt
     1021 agtagatatg tttgcatcta acaaacgtgt atggatgaaa aaacaaggta ctaatgaagt
     1081 taagtctgac atgagtattt tcaatattcc aagtgatgat ttaaacacag ttattggacg
     1141 tactaaagat aaaaatggta catcttgggt aggagagaat acaagagcac cgtatgtaac
     1201 agtaattggc gaatcggaag atggtttaac aggtcagccg gtatatgtag ccttacttaa
     1261 aggtactttt agtttagatt caattgaatt taaaacacga ggtgaaaaag cagaagcccc
     1321 agaacctaca aaattaacag gtgactggat gaatagaaaa gttgatgttg atggaacgtc
     1381 acaaggtatt gtatacggtt atcatgaagg taaagaagga gaagcagaat tcttcaaaaa
     1441 agtattcgtt ggatacacgg acagtgaaga tcattcagag gattctgcag gttcgttacc
     1501 cagctaatcc ccaaaatgtt gaagtagcag ttaattcaaa atctgcaaca gtttcagcag
     1561 aataggggct ttcaaaataa atcaaaggag aataatttat gactaaaact ttaaaggttt
     1621 ataaaggaga cgacgtcgta gcttctgaac aaggtgaagg caaagtgtca gtaactttat
     1681 ctaatttaga agcggataca acttatccaa aaggtactta ccaagtggca tgggaagaaa
     1741 atggtaaaga atctagtaaa gttgatgtac ctcaattcaa aaccaatcca attctagtct
     1801 caggcgtatc atttacacca gaaactaaat caattatggt aaataccgat gacaatgttg
     1861 agccaaacat tgcaccaagc acagcaacga ataaaatatt gaaatataca agtgaacatc
     1921 cagaatttgt tactgtagat gaaaatacag gagcaattca cggtgtagct gaaggtactt
     1981 cagtaatcac tgctatgtct actgatggaa gcgataagtc aggacaaatt tcagtgacag
     2041 taacaaacgg atagggattt aaggcgcagt atatctgcgt cttttttatt tgaataaaag
     2101 gagctaatac aatgattaaa tttgaaatta aagatcgtaa aacaggaaaa acagagagct
     2161 atacaaaaga agatgtaaca atgggcgaag cagaaaaatg ctatgagtat ttagaattag
     2221 taaatcaaga gaataaaaaa gaagcaccta acgcaacaaa aatgagacaa aaagagcgac
     2281 agttattagt agatttattt aaagatgaag gattgactga agaagatgtt ctgaacaaga
     2341 tgagtactaa aacttataca aaagccttac aagatatatt tcgagaaatc aatggtgaag
     2401 atgaagaaga ttcagaaact gaaccagaag agatgggaaa gacagaagaa caatctcaat
     2461 aaaagacatt ttatcgaaca ttaagaaaat acaacgtttc tgtatggagc agtatgggtg
     2521 gacattaact gaagtcagaa aacagccgta tgtaaaactt ttagaaatac ttaatgaaga
     2581 gaataaagaa gagactgaag aaaaacaaag tgaacaaaaa gtcattacag gtacggattt
     2641 aagaaaactt tttggaagct agaaaggagg ttaatatgaa tgaaaaagta gaaggcatga
     2701 ccttggagct gaaattagac catttaggtg tccaagaatg catgaaaggt ttaaagcgac
     2761 aattaggtgt tgttaatagt gaaatgaaag ctaatctgtc agcatttgat aagtctgaaa
     2821 aatcaatgga aaaatatcag gcgagaatta aggggttaaa tgataggctt aaagttcaaa
     2881 aaaagatgta ttctcaagta gaagatgagc ttaaacaagt taacgctaat taccaaaaag
     2941 ctaaatccag tgtaaaagat gttgagaaag catatttaaa gttagtagaa gccaataaaa
     3001 aagaaaaatt agctcttgat aaatctaaag aagccttaaa atcatcgaat acagaactta
     3061 aaaaagctga aaatcaatat aaacgtacaa atcaacgtaa acaagatgcg tatcaaaaac
     3121 ttaaacagtt gagagatgca gaacaaaagc ttaagaatag taaccaagct actactgcac
     3181 aactaaaaag agcaagtgac gcagtacaga agcagtccgc taagcataaa gcacttgttg
     3241 aacaatataa acaagaaggc aatcaagttc aaaaactaaa agtgcaaaat gacaatcttt
     3301 caaaatcaaa tgataaaatt gaaagttctt acgctaaaac taatactaaa ttaaagcaaa
     3361 cagaaaaaga atttaatgat ttaaacaata ctattaagaa tcatagcgct aatgtcgcaa
     3421 aagctgaaac agctgttaat aaagaaaaag ctgctttaaa taatttggag cgttcaatag
     3481 ataaagcttc atccgaaatg aagactttta acaaagaaca aatgatagct caaagtcatt
     3541 tcggtaaact tgcaagtcaa gcggatgtca tgtcaaagaa atttagttct attggagaca
     3601 aaatgacttc cctgggacgt acaatgacga tgggcgtatc tacaccaatt actttagggt
     3661 taggtgcagc attaaaaaca agtgcagact ttgaaggcca aatgtctcga gttggagcga
     3721 ttgcgcaagc aagcagtaaa gacttgaaaa gcatgtctaa tcaagcagtt gacttaggag
     3781 ctaaaaccag taaaagtgct aacgaagttg ctaaaggtat ggaagaattg gcagctttag
     3841 gctttaatgc caaacaaaca atggaggcta tgccaggtgt tatcagtgca gcagaagcaa
     3901 gtggtgcaga aatggctaca actgcaactg taatggcttc agcgattaac tctttcggtt
     3961 taaaagcatc tgatgcaaat catgttgctg atttacttgc gagatcagca aatgatagtg
     4021 ctgcagatat tcagtacatg ggagatgcat tgaagtatgc tggtactcct gcaaaagcat
     4081 taggagtttc aatagaggac acttccgcag caattgaagt tttatctaac tcaggtttag
     4141 agggttctca agcaggtact gccctaagag cttcatttat caggctagct aatccaagta
     4201 aaaatacagc taaggaaatg aaaaaattag gtattcattt gtctgatgct aaaggtcaat
     4261 ttgttggcat gggtgaattg attagacagt tccaagataa tatgaaaggc atgacgagag
     4321 aacaaaaact agctacagtg gctacaatag ttggtactga agcagcaagt ggatttttag
     4381 ccttgattga agcgggacca gataaaatta atagctatag taaatcctta aagaattcca
     4441 atggcgaaag taaaaaagca gcagatttga tgaaagataa tctcaaaggc gctctggaac
     4501 aattaggtgg cgcttttgaa tcattagcaa tcgaagtcgg taaagattta acgcctatga
     4561 ttagagcagg agcggaaggt ttaacaaaat tagttgatgg atttacacat ctccctggtt
     4621 gggttagaaa agcttcagta ggattagcac tttttggtgc agcaattgga cctgcagttc
     4681 ttgctggagg gttattaata cgtacagttg gaagtgctgc taaaggatat gcgtcattaa
     4741 atagacgtat tgctgaaaat acaatccttt caaatactaa ttcaaaagca atgaaatctt
     4801 taggtcttca aacattattt cttggttcta caacaggaaa aacgtcaaaa ggctttaaag
     4861 ggttagccgg agctatgatg tttaatttaa aacctataaa tgttttgaaa aattctgcaa
     4921 agctagcaat tttaccgttc aaacttttga aaaacggttt aggattagct gcaaaatctt
     4981 tatttgcagt aagtggaggc gcaagatttg cgggtgtagc cttaaggttt ttaacaggac
     5041 ctataggtgc tacaataact gctattacaa ttgcgtataa agtttttaaa accgcatatg
     5101 atcgtgtgga atggttcaga aacggtatta acggtttagg agaaactata aagttttttg
     5161 gtggtaaaat tattggcggc gctgttagaa agctaggaga gtttaaaaac tatcttggaa
     5221 gtatcggcaa aagcttcaaa gaaaagtttt caaaagatat gaaagatggt tataaatcat
     5281 taagcgacga tgaccttctc aaagtaggag tcaacaagtt taaaggattt atgcaaacca
     5341 tgggcacagc ttctaaaaaa gcgtctgata ctgtaaaagt gttagggaaa ggtgtttcaa
     5401 aagaaacaga aaaagcttta gaaaaatatg tgcattattc tgaagaaaat agcagaatca
     5461 tggaaaaagt acgtttaaac tcgggtcaga tatcagaaga caaagcaaaa aaacttttga
     5521 aaattgaaac ggatttatct aataacctta tagctgaaat agaaaaaaga aataaaaagg
     5581 aactcgaaaa aactcaagaa cttattgata agtatagtgc attcgatgaa caagaaaagc
     5641 aaaacatttt aactcgaact aaagaaaaaa atgacttgcg aattaaaaaa gagcaagaac
     5701 tcaatcagaa aatcaaagaa ttgaaagaaa aagctttgag tgatggtcag atttcagaaa
     5761 atgaaagaaa agaaattgaa aagcttgaaa atcaaagacg tgatatcact gttaaagaat
     5821 tgagtaagac tgaaaaagag caagagcgta ttttagtaag aatgcaaaga aacagaaatg
     5881 cttattcaat agacgaagcg agcaaagcaa ttaaagaagc agaaaaagca agaaaagcaa
     5941 gaaaaaaaga agtggacaag caatatgaag atgatgtcat tgctataaaa aataacgtca
     6001 acctttctaa gtctgaaaaa gataaattgt tagctattgc tgatcaaaga cataaagatg
     6061 aagtaagaaa agcaaaatct aaaaaagatg ctgtagtaga tgttgttaaa aagcaaaata
     6121 aagatattga taaagaaatg gatttatcca gtggacgtgt atataaaaat actgaaaagt
     6181 ggtggaatgg ccttaaaagt tggtggtcta actttagaga agaccaaaag aaaaaaagcg
     6241 ataaatacgc taaagaacaa gaagaaacag ctcgtagaaa cagagaaaat ataaagaaat
     6301 ggtttggaaa tgcttgggac ggcgtaaaaa gtaaaactgg cgaagccttt agtaaaatgg
     6361 gcagaaatgc taatcatttt ggcggcgaaa tgaaaaaaat gtggagcgga atcaaaggga
     6421 ttccaagcaa attaagttca ggttggagct cagccaaaag ttctgtagga taccacacta
     6481 aggctatagc taatagtact ggtaaatggt ttggaaaagc ttggcaatct gttaaatcga
     6541 caacaggaag tatttacaat caaactaagc aaaagtattc agatgcttca gataaagctt
     6601 gggcgcattc aaaatctatt tggagaggca catcaaaatg gtttagcaat gcatataaaa
     6661 gtgcaaaggg ctggctaacg gatatggcta ataaatctcg cgcgaaatgg gataatattt
     6721 ctagtacagc ttggtcgaat gcaaaatccg tttggaaagg aacatcgaaa tggtttagta
     6781 actcatacaa atctttaaaa gattggactg gggatatgta ttcaagagcc cacgatcgtt
     6841 ttgatgcaat ttcaagttcg gcatggtcta acgctaaatc agtatttaat ggttttagaa
     6901 aatggctatc caaaacatat gattggatta gagatattgg taaagacatg ggaagagctg
     6961 cggctgattt aggtaaaaat gttgctaata aagctattgg cggtttgaat agcatgattg
     7021 gcggtattaa taaaatatct aaagccatta ctgataaaaa tctcatcaag ccaataccta
     7081 cattgtctac tggtacttta gcaggaaagg gtgtagctac cgataattcg ggagcattaa
     7141 cgcaaccgac atttgctgta ttaaatgata gaggttctgg aaacgcccca ggtggtggag
     7201 ttcaagaagt aattcacagg gctgacggaa cattccatgc accccaagga cgagatgtgg
     7261 ttgttccact aggagttggg gatagcgtaa taaatgctaa tgacactctg aagttacagc
     7321 gtatgggtgt tttaccaaag tttcatggag gtacgaaaaa gaaagattgg ctagaccaac
     7381 ttaaaggtaa tataggtaaa aaagcaggag aatttggagc tacagctaaa aacacagcgc
     7441 ataatatcaa aaaaggtgca gaagaaatgg ttgaagcagc aggcgataaa atcaaagatg
     7501 gtgcatcttg gttaggcgat aaaatcggcg atgtgtggga ttacgtacaa catccaggga
     7561 aactagtaaa taaagtaatg tcaggtttaa atattaattt tggaggcgga gctaacgcta
     7621 cagtaaaaat agctaaaggc gcatactcat tgctcaaaaa gaaattagta gacaaagtaa
     7681 aatcgtggtt tgaagatttc ggtggtggag gcgatggaag ctatctattt gaatatccaa
     7741 tctggcaaag atttggacgc tacacaggtg gacttaactt taatggcggt cgtcactatg
     7801 gtatagactt tggtatgcct tctggaacaa acgtttatgc cgttaaaggt ggtatagcag
     7861 ataaggtatg gactgattac ggtggcggta attctataca aattaagact ggtgctaatg
     7921 aatggaactg gtatatgcat ttatctaagc aattagcaag acaaggccaa cgtattaaag
     7981 ctggtcaact gatagggaaa tcaggtgcta caggtaattt cgttagagga gcacacttac
     8041 atttccaatt gatgcaaggg tcacatccag ggaatgatac agctaaagat ccagaaaaat
     8101 ggttgaagtc acttaaaggt agtggcgttc gaagtggttc aggtgttaat aaggctgcat
     8161 ctgcttgggc aggcgatata cgtcgtgcag caaaacgaat gggtgttaat gttacttcgg
     8221 gtgatgtagg aaatattatt agcttgattc aacacgaatc aggaggaaat gcaggtataa
     8281 ctcaatctag tgcgcttaga gacatcaacg ttttacaggg caatccagca aaaggattgc
     8341 ttcaatatat cccacaaaca tttagacatt atgctgttag aggtcacaac aatatatata
     8401 gtggttacga tcagttatta gcgttcttta acaacagata ttggcgctca cagtttaacc
     8461 caagaggtgg ttggtctcca agtggtccaa gaagatatgc gaatggtggt ttgattacaa
     8521 agcatcaact tgctgaagtg ggtgaaggag ataaacagga gatggttatc cctttaacta
     8581 gacgtaaacg agcaattcaa ttaactgaac aggttatgcg catcatcggt atggatggca
     8641 agccaaataa catcactgta aataatgata cttcaacagt tgaaaaattg ttgaaacaaa
     8701 ttgttatgtt aagtgataaa ggaaataaat taacagatgc attgattcaa actgtttctt
     8761 ctcaggataa taacttaggt tctaatgatg caattagagg tttagaaaaa atattgtcaa
     8821 aacaaagtgg gcatagagca aatgcaaata attatatggg aggtttgact aattaatgca
     8881 atcttttgta aaaatcatag atggttacaa ggaagaagta ataacagatt ttaatcagct
     8941 tatattttta gatgcaaggg ctgaaagtcc aaacaccaat gataacagtg taactattaa
     9001 cggagtagat ggtattttac cgggcgcaat tagttttgcg cctttttcat tagtattaag
     9061 gtttggctat gatggtatag atgttataga tttaaattta tttgagcatt ggtttagatc
     9121 tgtgtttaat cgcagacatc cttattatgt tattacttct caaatgcctg gtgttaaata
     9181 tgcagtgaat acagctaatg ttacatctaa tttaaaagat ggttcttcaa ctgaaattga
     9241 agtaagttta aatgtttata aagggtattc tgaatcagtt aattggaccg atagcgagtt
     9301 cttattcgac tctaattgga tgtttgaaaa tggaattcct cttgatttca cacctaaata
     9361 tactcataca tcaaatcaat ttactatttg gaacggttct actgatacga taaatccacg
     9421 attcaagcac gatttgaaaa tattaattaa tttaaatgcg agtggaggat ttgaactggt
     9481 taactataca acaggtgata tttttaagta caacaaaagt atagataaaa acactgattt
     9541 tgttttagat ggtgtgtatg catatcgaga tataaataga gtgggaattg atacaaatag
     9601 aggcattata acattagcgc caggtaaaaa tgaatttaag attaaaggag acgtcagtga
     9661 tattaaaact acatttaagt ttccttttat ttataggtag gtgatttaat ggattatcat
     9721 gatcatttat cagtaatgga ttttaatgaa ttgatttgtg aaaatttact agatgtagat
     9781 tatggttctt ttaaagaata ttatgaactg aatgaagcta ggtacatcac ctttacagtt
     9841 tatagaacta ctcataatag ttttgttttt gatttattga tttgtgaaaa cttcataatt
     9901 tatcatggtg aaaaatatac aattaagcag acagcgccaa aggttgaagg tgataaagtt
     9961 tttattgaag ttacggcata tcacataatg tatgaatttc aaaatcactc agtggaatca
    10021 aataagcttg atgacgacag tagcgaaact ggtaaaacgc cagaatactc tttagatgag
    10081 tacttaagat atggatttgc aaatcaaaaa acttcggtca aaatgaccta taaaataatt
    10141 ggagatttta agcgaaaagt accgattgac gaattaggta acaaaaacgg cttagaatac
    10201 tgtaaagaag cggtagacct gtttggctgt ataatttacc caaatgatac agagattggt
    10261 ttttattctc ctgaaacatt ttatcaaaga agcgagaaag tgattcgata tcaatataat
    10321 actgatactg tatctgcaac tgtcagtaca ttggaattaa gaacagctat aaaagttttt
    10381 ggaaaaaagt atacagctga ggaaaagaaa aattataatc ctattagaac aactgacatt
    10441 aaatattcaa atggttttat aaaagaaggt acttatcgta ccgaaacaat tgggtctaaa
    10501 gctactatta actttgattg caagtatggt aatgaaacag ttagatttac aataaaaaag
    10561 ggctctcaag gtggaatata taagttgatt ttagacggca agcaaattaa gcaaatttct
    10621 tgttttgcta agtcggttca gtctgaaaca atagatttaa taaaaaatat tgataaaggc
    10681 aagcacgttt tagaaatgat atttttagga gaagacccca aaaatagaat tgatatatct
    10741 tcaaataaaa aagctaagcc ttgtatgtat gttggaactg aaaaatcaac agtcttaaat
    10801 ttaattgctg ataattcagg tcgcaatcaa tacaaagcaa ttgtcgacta cgtcgcagat
    10861 agtgcaaagc agtttgggat tcgatatgct aatacgcaaa caaatgaaga tatcgaaaca
    10921 caggataagc tgttagaatt tgcaaaaaag caaataaatg atactcctaa gactgaatta
    10981 gatgttaatt atataggtta tgaaaaaata gagccaagag atagcgtatt ttttgttcat
    11041 gaattaatgg gatataacac tgaattaaag gttgttaaac ttgataggtc acatccattt
    11101 gtaaacgcaa tagatgaagt gtctttcagc aatgaaataa aagatatggt acaaattcaa
    11161 caagcactta acagacgagt tattgcacaa gataatagat ataactatca agcaaatcgt
    11221 ataaatcatt tatacactag tactttgaat tctcctttcg agacaatgga tatagggagt
    11281 gtattaatat aatggcaaca gaagaagtta aaatcaaagc gctacttgaa aacgataaac
    11341 agtactttcc agctacacac tggaaagcta taaatgggat accttatgca ggcagtagtg
    11401 atattgatgg attgcctcaa gacggtatca tttcggtaga tgataaaaat aaattagata
    11461 aattaaaaat aggcgaagca ggaattattc aaaatagcat tgtacagaaa tccccaaacg
    11521 gtaaattgtg gaaaataaca gttgacgata gtgggaaact tggtacagtg ctattttatt
    11581 agaaaggaag gtgcattatg gaaaatttgt atttaataaa ggatttggga gctttagcag
    11641 gtcgagatta tagagctaaa gaaattcaaa acctgcaaag aatagagcaa tttgcgcttg
    11701 gcttgacaac agagtttaag ttgcatcaga aagctaaaac aatgcaacac ttcgctgagc
    11761 aaatttatta taatggtaga tcgcaagcag cagtaaacaa atctttacaa agtcaaatta
    11821 acgcacttgt tgtggcacca cgtaataaca gtgctaatga gattgttcaa gctcgagtta
    11881 atgtaaacgg cgaaaccttt gacacattaa aagaacattt agacgattgg gaaaccaaaa
    11941 ctcaaattaa taaagaggaa actataagag aattaaataa gaccaaacaa gaaattcttg
    12001 atatcgagta tcgttttgaa cctgataagc aagagttttt atttgtgaca gaacttgcac
    12061 ctcttacaaa tgcagtaatg caatccttct ggtttgataa tagaacaggc atagtataca
    12121 tgacacaagc tagaaataat ggctatatgc taagtcgttt aagacctaat ggtcaattta
    12181 tagacagctc attgattgta ggtgggggtc atggtacaca taacggttat agatatattg
    12241 atgatgagtt atggatttat agttttatct taaatggtaa taatgagaat acattagttc
    12301 gtttcaagta tacgcctaat gtggaaatta gctatggcaa gtatggtatg caagatgtat
    12361 ttacaggaca cccagaaaaa ccctacatca cccctgtcat aaatgaaaaa gaaaataaaa
    12421 ttctatacag aattgagaga cctagaagtc agtgggaact tgaaaactca atgaattata
    12481 tagagataag aagtttagac gatgttgata aaaatattga taaagttttg cataaaatca
    12541 gtatccctat gagactaaca aacgaaaccc aaccaatgca gggtgtgact tttgatgaaa
    12601 aatacttgta ttggtataca ggagacagta atccaaataa tagaaactat ttaacggctt
    12661 tcgatttaga aacaggagaa gaagcgtatc aggttaatgc tgactatggt ggaacactag
    12721 attcatttcc tggcgaattt gcggaagcag aaggtttgca aatatactat gacaaagata
    12781 gtggtaaaaa agctttgatg ctaggtgtta ctgtcggtgg tgatggaaat agaacacatc
    12841 gtattttcat gattgggcaa agaggtattt tagaaatact tcactcaaga ggcgttcctt
    12901 ttatcatgag tgacacaggt ggtagagtta aacctttacc aatgaggcct gataaactta
    12961 agaatcttgg gatgttaaca gagccaggtc tttactattt atacactgat catacagttc
    13021 aaatcgatga tttcccatta ccaagagaat ggcgtgatgc aggttggttc ttggaagtta
    13081 agccaccaca aactggcggt gatgtaattc agatattgac gcgtaatagt tatgcaagga
    13141 atatgatgac ttttgaaagg gtgctttctg gaagaactgg agacatttcg gactggaatt
    13201 atgtgcctaa aaatagtggt aaatgggaga gagtaccttc attcatcaca aaaatgtcag
    13261 atattaacat agtaggcatg tcgttttatt taactacgga tgatacaaaa cgttttacag
    13321 attttccaac tgaacgtaaa ggggtagctg gttggaactt atatgtagaa gcttcaaaca
    13381 caggtggctt tgttcatagg ctagttcgta atagtgttac agcatctgct gagatactat
    13441 tgaaaaatta tgatagtaaa acaagttcag ggccatggac tttacacgaa gggagaatta
    13501 taagttaatg agtaatttag agaaatctgt agctataaat ttagaaaaca cagcgcatta
    13561 tgaaaatatt tcaaatctag atataacttt tagaacagga gagagtgatt cttctgttct
    13621 tctttttaat atcattaaaa ataatcaacc gttattactg agtgaagaaa atatcaaagc
    13681 acgaatagcg attcgaggta aaggagtaat ggtagttgct ccactagaaa tattagatcc
    13741 atttaaaggt attttaaaat ttcaattacc taatgatgta attaaaagag atggaagtta
    13801 tcaagctcaa gtttcggttg cagaattagg taattcagac gtggtagttg tagagagaac
    13861 tatcacattt aacgttgaaa aaagtttgtt tagcaagatt ccctctgaaa caaaactaca
    13921 ctatattgtt gagtttcaag aattagaaaa aactattatg gatcgcgcga aagcaatgga
    13981 cgaggctata aaaaatggtg aggattatgc gagtctgatt gaaaaagcta aagaaaaagg
    14041 tctatcagat attcaaatag caaaatcttc aagtattgat gaattaaagc aacttgctaa
    14101 tagccgtata tctgatttgg aaaataaagc gcaagcatat tcaagaacat tcgatgagca
    14161 aaagcgatat atggatgaga aacatgaagc cttcaagcag tcagtgaata gtggtggttt
    14221 agtcacaagt ggttctactt caaattggca aaaagctaag attactaaag atgatggtaa
    14281 gataatgcag attactggat ttgattttaa taatccagaa caaagaatag gtgattcaac
    14341 ccaatttatt tatgtttcgc aagctataaa ttatccaaga gatgttagta ctaacggtac
    14401 tgtcgaatat ttagtagtaa cttcagatta caagcgtatg acttatcgac cgaacggtac
    14461 aaataaagtg tttgttaaaa gaaaagaagc gggttcatgg tctgagtggt cagaattagc
    14521 tattaatgat tacaatacac cttttgaaac tgttcaaagt gcccaatcaa aagctaatat
    14581 ggccgaaagt aacgctaaat tatacgcaga tgacaagttt aataaaaggt attcggttat
    14641 ttttgatgga acagcaaatg gtgtgggctc tacattgtac ttaaatgaga gtttagacca
    14701 atttatttta ttaatttttt atgggacttt tccaggtggt gactttacag agtttggcag
    14761 tccttttgga ggaggaaaga tttcattgaa tccctcaaat cttccagatg gtgatggaaa
    14821 tggtggaggt gtttatgagt ttggattaac taaatctagt cgtacatctt taactatatc
    14881 aaacgatgtc tatttcgact taggaagtca aagaggctct ggtgcgaacg caaatagagg
    14941 gacaattaac aaaattatag gagtgagaaa ataatgcaaa tattagttaa caagcgtaat
    15001 gagataattt catacgctat cattggcggc tttgaagaag gtattgatat tgaaaattta
    15061 ccagaaaatt tctctcaagt ttttagacct aaagccttta aatattcaaa tggggaaata
    15121 gtttttaacg aagattattc agaagaaaaa gatgacttgc atcaacagat tgacagtgaa
    15181 gaacaaaaca cagtcgcttc tgatgacatc ttacgaaaaa tggttgctag tatgcagaaa
    15241 caagttgttc aaagtacaaa gttatcgatg caagttaata agcaaaatgc actaatggca
    15301 aaacaacttg tgacacttaa taaaaaatta gaagaggtta aaggagagac tgaaaatgct
    15361 taaattaatt tcaccaacat tcgaagatat taaaacatgg tatcaattga aagaatatag
    15421 taaagaagat atagcgtggt atgtagatat ggaagttata gataaagagg aatatgcaat
    15481 tattacagga gaaaagtatc cagaaaatct agagtcatag gttataatct tatggctttt
    15541 taatttgaat aaagtgggtg gtgtaatgtt tggatttacc aaacgacacg aacaagattg
    15601 gcgtttaacg cgattagaag aaaatgataa gactatgttt gaaaaattcg acagaataga
    15661 agacagtctg agaacgcaag aaaaaattta tgacaagtta gatagaaatt tcgaagaact
    15721 aaggcgtgac aaagaagaag atgaaaaaaa taaagagaaa aatgctaaaa atattagaga
    15781 catcaagatg tggattctag gattaatagg gacgattcta agtacatttg ttatagcctt
    15841 gttaaaaact atttttggca tttaaaggag gtgatcacca tgcttaaggg aattttagga
    15901 tatagctttt ggtcgtgttt ctggtttggt aagtgtaagt aatatttaag agtcagtgct
    15961 tcggcactgg ctttttattt tggataaaag gagcaaacaa atggatgcaa aagtaataac
    16021 aagatacatc gtattgatct tagcattagt aaatcaattc ttagcgaata aaggtataag
    16081 tccgatacca gtagatgaag aaagtgtttc atcgattatc ttaacagttg ttgctttata
    16141 tactacatat aaagataatc caacatctca agaagggaaa tgggcgaatc aaaaattaaa
    16201 gaaatataaa gctgaaagta aatatagaaa agcaacaggg caagcgccaa ttaaagaagt
    16261 aatgacacct acgaatatga acgacacaaa tgatttaggg taggtgttga ccaatgttga
    16321 taacaaaaaa ccaagcagaa aaatggtttg ataattcatt agggaagcag ttcaatcctg
    16381 atttgtttta tggatttcag tgttatgatt acgccaatat gttctttatg ttagcgacag
    16441 gcgaaaggct gcaaggttta tatgcttata atatcccgtt tgataataaa gcaaagattg
    16501 aaaaatatgg tcaaataatt aaaaactatg acagcttttt accgcaaaag ttggatattg
    16561 tcgttttccc gtcaaagtat ggtggcggag ctggacacgt tgaaattgtt gagagcgcaa
    16621 atttaaacac tttcacatca tttggtcaaa actggaacgg taaaggttgg actaatggcg
    16681 ttgcgcaacc tggttggggt cctgaaactg tgacaagaca tgttcattat tatgacaatc
    16741 caatgtattt tattaggtta aacttcccta acaacttaag cgttggcaat aaagctaaag
    16801 gtattattaa gcaagcgact acaaaaaaag aggcagtaat taaacctaaa aaaattatgc
    16861 ttgtagccgg tcatggttat aacgatcctg gagcagtagg aaacggaaca aacgaacgcg
    16921 attttatacg taaatatata acgcctaata tcgctaagta tttaagacat gcaggacatg
    16981 aagttgcatt atacggtggc tcaagtcaat cacaagatat gtatcaagat actgcatacg
    17041 gtgttaatgt aggcaataaa aaagattatg gcttatattg ggttaaatca caggggtatg
    17101 acattgttct agaaatacat ttagacgcag caggagaaag cgcaagtggt gggcatgtta
    17161 ttatctcaag tcaattcaat gcagatacta ttgataaaag tatacaagat gttattaaaa
    17221 ataatttagg acaaataaga ggtgtaacac ctcgtaacga tttactaaat gttaatgtat
    17281 cagcagaaat aaatatcaat tatcgcttat ctgaattagg ttttattact aataaaaatg
    17341 atatggattg gattaagaaa aattacgact tgtactctaa actaatagct ggtgcgattc
    17401 atggtaagcc aattggtgga gtggtagcta gtgaggtcaa agcgccagtt aaaaacgaaa
    17461 agaatccgcc agtgccagca ggttatacac tcgataagaa taatgtccct tataaaaaag
    17521 aacaaggcaa ttacacagta gctaatgtta aaggtaataa tgtaagagac ggttattcaa
    17581 ctaattcaag aattacaggg gtattaccca acaacacaac aattacgtat gacggtgcat
    17641 attgtattaa tggttataga tggattactt atattgctaa tagtggacaa cgtcgctata
    17701 ttgcgaccgg agaggtagac atagcaggca accgaataag cagttttggt aagtttagtg
    17761 cagtttgata attgtatatg atgaatctta ggcaggtact tcggtacttg cctattattt
    17821 aaaattaata aacagttaat ttttacatga atatattaaa ttttaaaaaa acaaacgttt
    17881 ttagtatata aattattttg tgttcgtatt gtgtgctatg attaaaaagt tgttatggtc
    17941 aactatatcg tggttttatg tttattatca atcaaaatat aaattattta taatttgttt
    18001 ggtaatgaac gggttttttt cgaaataata gtaaaaaaac acatttgta
//
LOCUS       DALAGD010000066        17523 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.067, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000066 DALAGD010000000
VERSION     DALAGD010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17523
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.067"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(99..857)
                     /locus_tag="O2701_001806"
     CDS             complement(99..857)
                     /locus_tag="O2701_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCZ4293694.1"
                     /translation="MSEFKTGKINKHVLYSNILNRDVTLSIYLPESYNQLVKYNVILC
                     FDGLDFLRFGRIQRTYESLIKEARIDDAIIVGFHYEDVDKRREEFHPQGSRSHLTIQS
                     VGKEILPFIDSTFSTLKVGNARLLVGDSLAGSIALLTALTYPTIFSRVAMLSPHSDEK
                     VLDKLNQCANKEQLTIWHVIGLDEKDFTLPTNGKRADFLTPNRELAEQIKKYNITYYY
                     DEFDGGHQWKDWKPLLSDILLYFLSKNTDDQLYE"
     gene            complement(999..2567)
                     /locus_tag="O2701_001807"
     CDS             complement(999..2567)
                     /locus_tag="O2701_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HCZ4293695.1"
                     /translation="MIEKLVTFLNEVVWSKPLVYGLLITGVLFTLRMRFFQVRHFKEM
                     IRLMFQGEKSPNGISSFQAIAMSLAGRVGTGNIVGVSTAIFIGGPGAVFWMWITAFLG
                     ASSAFIESTLGQIFKRVENNEYRGGPAYYIEYGIGGKFGKIYGIIFAIVTIISVGLLL
                     PGVQSNAIASSMHNAIHVPQWLMGGIVVVILGLIIFGGVRSIANVATAVVPFMAIIYI
                     LMAVIIICINIQEVPALFALIFKSAFGLQSAFGGIVGAMIEIGVKRGLYSNEAGQGTG
                     PHAAAAAEVSHPSKQGLVQAFSVYIDTLFVCTATALIILISGTYNVTDGTVNANGTPH
                     LIKDGGIYVENATGKDYSGTAMYAQAGIDKAFHGSGYQFDPTFSGVGSYFIAFALFFF
                     AFTTILSYYYITETNVAYLTRNQNNQVSSIFINIARVIILFATFYGAVKTADVAWAFG
                     DLGVGLMAWLNIIAIWILHKPAVNALKDYEIQKKRLGNGYNAVYQPDPNKLPNAVFWL
                     KTYPERLKQARAKK"
     gene            2909..3994
                     /locus_tag="O2701_001808"
     CDS             2909..3994
                     /locus_tag="O2701_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCZ4293696.1"
                     /translation="MLNKVWFRTGIALIMLFILIKLFMEVHEVFTPIATIIGSVFLPF
                     LISGFLFYICLPFQNLLEKVGFPRWASITTIMLALFAIIGLIVAFVAPIIISNINNLI
                     SQTPALQKEAEQIIKFALAQMDKLPEDVTSRITNMVKSMGDGVTNILSNSLQYITSLI
                     STIFLLIMVPFFLIYMLKDHEKFIPAVAKFFKGERKVFFVDLLTDLNFTLKSYIQGQV
                     TVSVILGIFLYIGYSIIDLPYIPLLVLFAGVANLIPFLGSWLSFAPAAILGIIDSPTT
                     FIWVCIITLIAHQLEGNIITPNVMGKSLSIHPLTIIVVILAAGDLGGFTLVLIAVPLY
                     AVLKTVVSNIFKYRQRIIDKANSNVKD"
     gene            complement(4189..4959)
                     /gene="fabI"
                     /locus_tag="O2701_001809"
     CDS             complement(4189..4959)
                     /gene="fabI"
                     /locus_tag="O2701_001809"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCZ4293697.1"
                     /translation="MLNLENKTYVIMGIANKRSIAFGVAKVLDQLGAKLVFTYRKERS
                     RKELEKLLEQLNQPEAHLYQIDVQSDEEVINGFEQIGKDVGNIDGVYHSIAFANMEDL
                     RGRFSETSREGFLLAQDISSYSLTIVAHEAKKLMPEGGSIVATTYLGGEFAVQNYNVM
                     GVAKASLEANVKYLALDLGPDNIRVNAISAGPIRTLSAKGVGGFNTILKEIEERAPLK
                     RNVDQVEVGKTAAYLLSDLSSGVTGENIHVDSGFHAIK"
     gene            complement(5237..7081)
                     /locus_tag="O2701_001810"
     CDS             complement(5237..7081)
                     /locus_tag="O2701_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter family
                     protein"
                     /protein_id="HCZ4293698.1"
                     /translation="MEFLSLVIVVLAAFLTPIIVNRLNINFLPVVVAEILMGIVIGNS
                     FLNIVERDSILNILSTLGFIFLMFLSGLEIDFKAFKKDKRARQGQNDDESSIPGHLNL
                     ALTVFAFIMIISILLAYVFKWLGLVDDVLLMVIIISTISLGVVVPTLKEMNIMRTTIG
                     QFILLVAVLADLVTMILLTVYGAINGQGGSTIWLIGILVVFTAISYILGVQFKRMSFL
                     QKLMDGTTQIGIRAVFALIILLVALAEGVGAENILGAFLAGVVVSLLNPDEEMVEKLD
                     SFGYGFFIPIFFIMVGVDLNIPSLIKEPKLLIIIPILIVAFIISKLIPVMFIRRWFDM
                     KTTIASAFLLTSTLSLVIAAAKISERLNAISAETSGILILSAVITCVFVPIIFKKLFP
                     VPDEFNRKIEVSLIGKNQLTIPIAQNLTSQLYDVTLYYRKDLSDRRQLSDDITMIEIA
                     DYEQDVLERLGLFDRDIVVCATNDDDINRKVAKLAKAHQVERVICRLESTTDDTELVD
                     SGIEIFSSYLSNKILLKGLIETPNMLNLLSNVETSLYEIQMLNYKYENIQLRNFPFGG
                     DIIFVRIIRNNESIVPHGDTQLRYGDRLIVTGAKEYVDELKQELEFYF"
     gene            complement(7091..8476)
                     /gene="mgtE"
                     /locus_tag="O2701_001811"
     CDS             complement(7091..8476)
                     /gene="mgtE"
                     /locus_tag="O2701_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HCZ4293699.1"
                     /translation="MSMNTDEKERVQEELYDQTLLDQYLENDDIDQFRDEFLALHTYE
                     QSEYFEDTTDENRQKIFQYLSPEEVANFFDQLDIDDDEYELLFDKMNATYASHILEEM
                     SYDNAVDILNELTKPKVASLLTLMNKDDANEIKALLHYDEDTAGGIMTTEYLSLKAHT
                     PVKEALLLVKAQAPDAETIYVIFVVDDDGKLVGVLSLRDLIVAENDAYIEDIMNERVI
                     SVNVADDQEDVAQVMRDYDFMAVPVIDYQEHLLGIITIDDILDVMDEEASEDYSRLAG
                     VSDIDSTNDSIIKTALKRLPWLIILTFLGMITATILGRFEKTLENVALLAAFIPIISG
                     MSGNSGTQSLAVSVRNITTGEINEQSKFRIALREAGSGVLSGVVCSTILFTIIVAIYH
                     QPLLALIVAGSLTCAMTVGTFVGSMIPLLMNKLNIDPAVASGPFITTINDIISMLIYF
                     GLATSFMAYLI"
     gene            complement(8497..9351)
                     /locus_tag="O2701_001812"
     CDS             complement(8497..9351)
                     /locus_tag="O2701_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCZ4293700.1"
                     /translation="MKFKYHISQQETVKTFLARHDFSKKTVSAIKNNGALIVNDEPVT
                     VRKQLMTNDILEIHLPREIPSVNLIPYARKLEVLYEDAFIIIVTKPNNQNCTPSREHP
                     HESLIEQVLYHCQEHGENINPHIVTRLDRNTTGIVIFAKYGHIHHLFSKVNLKKIYTC
                     LVYGKTHTSGIIEANIRRSKDRIITREVASDGKYAKTSYEVINQNDKYSLCKVHLHTG
                     RTHQIRVHFQHIGHPIVGDSLYDGFHDKIHGQVLQCTQIYFVHPINKNNIYITIDYKQ
                     LLKLFNQL"
     gene            complement(9348..10157)
                     /locus_tag="O2701_001813"
     CDS             complement(9348..10157)
                     /locus_tag="O2701_001813"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HCZ4293701.1"
                     /translation="MRYTILTKGDSKSNALKHKMMNYMKDFRMIEDSENPEIVISVGG
                     DGTLLQAFHQYSHMLSKVAFVGVHTGHLGFYADWLPHEVEKLIIEINNSEFQVIEYPL
                     LEIIMRYNDNGYETRYLALNEATMKTENGSTLVVDVNLRGKHFERFRGDGLCVSTPSG
                     STAYNKALGGALIHPSLEAMQITEIASINNRVFRTVGSPLVLPKHHTCLISPVNHDTI
                     RMTIDHVSIKHKNVNSIQYRVANEKVRFARFRPFPFWKRVHDSFISSDEER"
     gene            complement(10174..10809)
                     /locus_tag="O2701_001814"
     CDS             complement(10174..10809)
                     /locus_tag="O2701_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCZ4293702.1"
                     /translation="MNQWDQFLTPYKQAVDELKVKLKGMRKQYEVGEQASPIEFVTGR
                     VKPIASIIDKANKRQIPFDRLREEMYDIAGLRMMCQFVEDIDVVVNILRQRKDFKVIE
                     ERDYIRNTKESGYRSYHVIIEYPIETLQGQKFILAEIQIRTLAMNFWATIEHTLRYKY
                     DGAYPDEIQHRLERAAEAAYLLDEEMSEIKDEIQEAQKYYTQKRSKKHEND"
     gene            complement(10826..11173)
                     /locus_tag="O2701_001815"
     CDS             complement(10826..11173)
                     /locus_tag="O2701_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293703.1"
                     /translation="MRLYINEIKIKDDILYCYTEDSIKGLSEVGQMLVDSDNYAFAYT
                     LDDGKAYAYLIFVQETWTMLHENMTKKIIINDELELTEFHQELTYILDNIKGNNNYGK
                     EFVATVEETFDIE"
     gene            11359..11952
                     /locus_tag="O2701_001816"
     CDS             11359..11952
                     /locus_tag="O2701_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HCZ4293704.1"
                     /translation="MATNHEIEFKQMITASIYNKLQEKYFKDSVLFKQVNYYIDTPDF
                     KLKEHRSALRIRVKDNQYEMTLKTPAKVGLLEYNYIVDIKPEMNLIISNDNLPDDIRQ
                     IIVEQFGVKDTTLSILGALTTYRQETKYKGDLLVLDKSEYFDTTDYELEFEVKDYNQG
                     LQKFQSLLNELNLEHHQPLNKVQRFFKKKETLSNNIN"
     gene            12056..12421
                     /locus_tag="O2701_001817"
     CDS             12056..12421
                     /locus_tag="O2701_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="truncated hemoglobin"
                     /protein_id="HCZ4293705.1"
                     /translation="MTTTPYDIIGKEALYDMIHYFYTLVEKDERLNHLFPGDFAETSR
                     KQKQFLTQFLGGPNIYTEEHGHPMLRKRHIDFTITEFERDAWLENMQTAINRAAFPQG
                     VGDYLFERLRLTANHMVNS"
     gene            12444..13250
                     /gene="yjbH"
                     /locus_tag="O2701_001818"
     CDS             12444..13250
                     /gene="yjbH"
                     /locus_tag="O2701_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease adaptor protein YjbH"
                     /protein_id="HCZ4293706.1"
                     /translation="MAGELRIMENKSREDINLSPVSKIEIYSFFDPFSSDCFKLSAIL
                     SKLRIEYNQYIRIRHILNPSLKVLTKCQAQSTSNFDNIALAYKAAELQGRVRAERFIH
                     LMQNEIIPKRDIITESMICDCIQNAGIDLEVFKDDLQKSKLTESLKIDLHIAREMEIE
                     QAPSLVFFSEDVHEEGLKVEGLYPYHIYTYIINELMGKPIEKNLPPKLETYIQQQQLV
                     TMEELLTIYEWPEKLLNKELKKLAIQQKIEKLKYPDGDFWKSKMPKIKSK"
     gene            complement(13710..15518)
                     /gene="pepF"
                     /locus_tag="O2701_001819"
     CDS             complement(13710..15518)
                     /gene="pepF"
                     /locus_tag="O2701_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCZ4293707.1"
                     /translation="MSQQLSREEQERKYPEYTWDLTTIFKDDEAFEAAFKEVENELGK
                     EEQFKGHIGDSAETLYNALELEDTLGTKLEKVYVYAHLKQDQDTTNDKYTGMESRAHQ
                     LIIKFSSAWSFLVPEILQIDEDKIQSFVNSYDKLQKFAFDLKLINEKRPHILDAETEK
                     LLTEAQDALSTPSNVYGMFSNADLVFEDAIDKDGNAHPLTQGTFIKYLETDDRKLRES
                     AFRNVYKAYGAHNNTLGATLAGEVKKNVFNARTHNYKTAREKALSNNHIPENVYDNLV
                     KTVHKYLPLLHRYTELRKELLGLDDLKMYDLYTPLIKDIKFEMPYEEAKEWMLKALEP
                     MGEEYLNVVKEGLNNRWVDVYENKGKRSGGYSSGAHLTNPFILLNWSNTISDLYTLVH
                     EFGHSAHSYFSRKFQPSNSSDYTIFVAEVASTCNEALLSDYMDKHLDDEKRLLLLNQE
                     LERFRATLFRQTMFAEFEHKIHAIEEAGEPLTPTRMNEEYAKLNKLYFGDSVETDEDI
                     SKEWSRIPHFYMNYYVYQYATGYSAAQSLSHQILTEGKPAVDRYINEFLKKGSSNYPI
                     EILKNAGVDMTTPEPIEQACEVFEQKLNAFEKLMKA"
     gene            complement(15566..16551)
                     /locus_tag="O2701_001820"
                     /pseudo
     CDS             complement(15566..16551)
                     /locus_tag="O2701_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499489.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
     gene            complement(16672..17391)
                     /gene="mecA"
                     /locus_tag="O2701_001821"
     CDS             complement(16672..17391)
                     /gene="mecA"
                     /locus_tag="O2701_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HCZ4293708.1"
                     /translation="MRIERVDDTTVKLFITYSDIEARGFSREDLWTNRKRGEEFFWSM
                     MDEINEEEDFVVEGPLWIQVHAFEKGVEVTISKSKNEDMMNMSDDDATDQFDEQVQEL
                     LAQTLEGEDQLEELFEQRTKEKEAQGSKRQKSSARKNTRTIIVKFNDLEDVINYAYHS
                     NPITTEFEDLLYMVDGTYYYAVYFDSHVDQEVINDSYSQLLEFAYPTDRTEVYLNDYA
                     KIIMSHNVTAQVRRYFPETTE"
BASE COUNT         5900 a         3132 c         2388 g         6103 t
ORIGIN      
        1 cgtcaaattt aaattaaatt aaacagtaaa atcttttata aattatatta tagaccagac
       61 tatatgaaga caattcatac taaatctact aatgtaaatt attcataaag ttgatcatct
      121 gtgtttttac ttaaaaaata caataatata tctgacagca atggtttcca atctttccat
      181 tggtgaccac catcaaattc atcgtaataa taagttatat tatatttctt aatttgttca
      241 gctaattctc tattcggtgt taagaaatcg gcacgcttac catttgttgg taaagtaaaa
      301 tctttttcat ctagaccaat gacatgccaa attgtcaatt gttctttatt tgcacattga
      361 tttagcttat ctaatacttt ttcatctgaa tgtggactta acattgctac acgactaaaa
      421 atcgttggat aggtcaacgc cgttaataag gcaatactac ccgctaaact atcccctact
      481 aataaccttg cattacctac tttcagtgta gaaaacgtcg agtcaataaa tggcaatatt
      541 tctttaccga ctgattgaat agttaaatga gaacgacttc cttgtggatg aaattcctct
      601 ctacgcttat caacgtcttc ataatggaat ccaacaatga tcgcatcatc aatacgcgct
      661 tctttgatta acgattcata tgtacgttgt attctcccga aacgtaaaaa atctaatccg
      721 tcaaagcaaa gaatgacatt atatttaaca agttgattat aagattctgg taaataaata
      781 cttaacgtga catctctatt taaaatatta ctatataaaa catgtttatt aatcttacca
      841 gttttaaatt ctgacataaa aaagtgcccc tagtagttgt attgttattg taccaagaga
      901 cactatattt ttaaaattca aatttctgag cacctaaaat tctcaatact tttctttttt
      961 atcaaatgat cactacatat actataaaca aaagtagatt actttttggc acgtgcttgt
     1021 tttaaacgtt ctggatacgt cttcaaccaa aagacagcat taggtaattt attcggatca
     1081 ggttgataaa ctgcattata accgttgcct aaacgtttct tttgaatttc ataatctttt
     1141 aaagcattta cggcaggctt atgtaaaatc caaatcgcaa tgatatttaa ccaagccatt
     1201 agacctacac ctaaatcacc gaatgcccat gctacatcag ctgttttaac tgcaccgtaa
     1261 aatgtagcga acaaaataat cacacgagca atattaataa atatcgatga aacttgatta
     1321 ttttgattac gcgttaaata agcaacattt gtttctgtaa tgtagtagta cgacaaaatt
     1381 gtagtaaatg caaagaagaa taaagcaaat gcaataaagt acgaacctac gccagagaaa
     1441 gtaggatcaa attgataacc actgccatgg aacgctttat caatgccggc ttgtgcatac
     1501 atcgcagtac ctgaataatc tttacctgtt gcattttcaa cataaatacc gccatcttta
     1561 attaaatgcg gtgtgccatt cgcattaacc gtaccatcag tcacattata tgtaccagaa
     1621 ataagtataa tcagagcagt tgcagtacat acaaataatg tatcaatata aactgaaaat
     1681 gcttgtacta gaccttgttt acttggatgt gatacttctg ctgccgctgc tgcgtgtgga
     1741 cctgtacctt gaccagcctc atttgaatat aatccacgtt taacaccaat ctctatcatt
     1801 gcgccaacga taccaccaaa agcagattgt aatccaaatg ctgatttgaa aattaatgca
     1861 aataacgctg gcacttcttg tatattgata caaatgataa tgacagccat cagtatgtaa
     1921 ataattgcca taaatggtac aacggctgtt gcaacattgg caatactacg tacaccacca
     1981 aaaataatta atcccaaaat aactacaaca ataccaccca ttaaccattg tggaacatga
     2041 atcgcattat gcatagaact tgctatagcg ttagattgca caccaggaag caatagacct
     2101 actgagataa tcgtaacaat agcaaagata attccgtaaa ttttaccaaa tttaccacca
     2161 ataccatatt caatataata cgctggtcca ccacggtatt cattattttc aactctcttg
     2221 aatatttgac caagtgtaga ttcaataaaa gcactacttg cacctaaaaa cgcagtaatc
     2281 cacatccaaa atactgcacc aggtcctcct ataaatattg cagtagatac accgacaata
     2341 ttacctgtac caaccctgcc tgctaaagac atggctatcg cttgaaaact tgaaatacca
     2401 ttaggagact tctctccttg aaacattaat cgaatcattt ctttaaaatg tctaacttga
     2461 aaaaatcgca tacgcaatgt aaatagcaca ccagtaatta gcaaaccata aactaatggc
     2521 ttactccaaa caacctcatt taaaaaggtt actaattttt caatcataag attcccccta
     2581 ttgtttaaac ataaaaatat tatacgatta agcacgagac acttcaatat atttttcaga
     2641 atattcttat aaatattaat atgatcattt cactatttaa acacgtttaa tattaaaata
     2701 agttattcat ataaactggt gctgtttgat ccaattgcag acttacgagt cattgaaaag
     2761 tctcacaaac tattgaaagt aaatatctta aaatagaaag tgaatggtta atttaagtat
     2821 atttaaaaaa tattaacctt tttaagcact gctatttagg atatactaaa taataactaa
     2881 gtttagaaaa ataggaggaa catcgtttat gttaaacaag gtttggttcc gaactggcat
     2941 cgctctgatt atgctgttca ttctcatcaa actatttatg gaagtgcacg aagtatttac
     3001 tccaatagct acaatcatcg gttctgtatt tcttccattt ttaattagtg gttttttatt
     3061 ttatatctgt ctaccttttc aaaacttact tgaaaaagtc ggttttccaa gatgggctag
     3121 tataacaaca atcatgttag ctttgtttgc tattatcggc ttaattgttg cgtttgttgc
     3181 accaatcatc atttcaaata tcaacaattt aattagtcag acacctgccc tacaaaaaga
     3241 agcagagcaa attattaaat tcgcactagc tcaaatggat aaattacctg aagatgtaac
     3301 aagtagaatt acaaatatgg ttaaatcaat gggtgatggc gttactaata ttttatctaa
     3361 ttcattacag tacattacat cattaatttc aaccatcttc ttattgatta tggttccatt
     3421 cttcttaatt tatatgttaa aagaccatga aaaatttatc ccagctgttg ctaaattttt
     3481 caaaggtgaa cgtaaagtat ttttcgtaga tttattaact gatttaaact ttacattaaa
     3541 atcttacatt caaggtcaag taactgtcag tgttatttta ggtatattct tatatatcgg
     3601 ttattcaatt attgacttac catatattcc tttattagta ctatttgctg gtgtcgcaaa
     3661 cttaattcct ttcttaggtt catggttatc atttgcacct gcagcaatac tcggtattat
     3721 agatagtcct actacattta tatgggtttg tatcattaca ttaattgcac atcaacttga
     3781 aggtaatatc attacaccaa atgtaatggg taaatcttta agtatccatc ctttaacaat
     3841 tatcgttgtt attttagcag caggagattt aggtggcttt acattagttc tgattgcagt
     3901 gccattatat gctgtactta aaacggttgt tagtaatatt ttcaaatacc gccaacgcat
     3961 tattgataaa gcaaacagta atgttaagga ctaattctgt ggatgtcttt taagaatata
     4021 agatactatc gcatcaaaag ttgaaactac agcttttgag gcgatttttt tgtgcataaa
     4081 aaatcagtca aatgaaatat caaataattt tccatcagtc cgattattat aaaagcaaaa
     4141 aagctttgct cacatatata ataacgtgaa caaagctgtt gaatgatatt atttaattgc
     4201 gtggaatccg ctatctacat gaatattttc acctgtaacg ccacttgata aatcacttaa
     4261 taagtaagcc gcagttttac ctacttctac ttgatcaaca ttacgtttta aaggtgcacg
     4321 ctcttcgatt tctttaagaa ttgtattgaa accacccaca ccttttgcac ttaatgtacg
     4381 gattggacca gctgaaattg cattaacgcg aatattatct ggacctaagt ctaatgctaa
     4441 atatttaaca tttgcttcta agctcgcttt agcaacaccc atcacattat agttttgaac
     4501 tgcgaattcg ccacctaaat atgttgttgc aacaatgcta ccaccttctg gcattaattt
     4561 tttagcttca tgagccacaa ttgttaatga gtaagaacta atgtcttgag ctaacaagaa
     4621 gccttcacgt gaagtttcag aaaagcgtcc gcgtaagtct tccatattag caaatgcgat
     4681 tgaatgatat acaccatcaa tattgccaac atctttacca atttgctcaa aaccattaat
     4741 aacctcttca tcgctttgaa catcaatttg atataagtgc gcttctggtt gatttaattg
     4801 ttctaataat ttttcaagct ctttacggct acgttcttta cggtaagtaa atactaattt
     4861 agcacctaat tgatctaaaa ctttagcgac accaaaagca atactacgct tattagcgat
     4921 tcccatgatg acatatgttt tgttttcaag atttaacatg taagataact ccttataata
     4981 attaatttaa cggatagaat atctaatccc agttttaact aactttttat tcgtcaatac
     5041 attttaacac tattatgact ttttaataaa gttatttaaa cgtattacaa cgataaatgt
     5101 ttgatttagt cctttctgta tcaaacttga tttacgtaaa aagcatttat ttatcaaaat
     5161 catatacata ctagcatttt aaaatcatta attttacgtc gtactaaaat acgtctgcat
     5221 tttaatatca ttattgttaa aaataaaatt ctaactcttg cttcaattca tcaacgtatt
     5281 ctttagcacc ggtaacaatt aagcgatctc catatcgcaa ttgtgtatct ccatgcggaa
     5341 caatcgactc attattacgg ataatacgca cgaagatgat gtctcctccg aatgggaaat
     5401 tacgtaattg aatattttca tatttataat ttaacatttg aatttcatat agtgacgttt
     5461 caacattact taataaattc aacatgttag gtgtttcaat taaacctttt aataagattt
     5521 tattacttaa gtagctactg aaaatttcaa tacctgaatc aactaactct gtatcgtccg
     5581 ttgtgctttc aagtctgcaa atgacacgct caacttgatg tgctttggct aatttagcaa
     5641 cttttcggtt aatatcatcg tcattcgtag cacaaacaac tatgtctcgg tcaaacagac
     5701 ctagtcgttc taaaacatct tgttcataat cagcaatttc tatcatcgtg atatcatctg
     5761 acaattgacg acgatcactc aagtctttgc gataatataa tgtcacgtca tataactgag
     5821 atgttaaatt ttgcgctata ggaatcgtta attgattttt accaattaaa ctaacttcaa
     5881 ttttacggtt aaactcatct ggaactggaa acagtttttt gaaaataatc ggaacgaata
     5941 cacatgtaat gactgcgctt aaaattaata tacctgacgt ttcagctgaa atagcattta
     6001 atctttctga aattttggct gcagctatca cgagcgataa tgttgatgtt aataaaaatg
     6061 ctgatgcaat cgttgttttc atatcaaacc aacgtcgaat aaacatgact ggaattaatt
     6121 ttgaaatgat aaatgcaacg attaaaatcg gtatgataat tagtaatttc ggttctttaa
     6181 ttaatgaagg tatgtttaaa tctacaccaa ccattataaa gaaaatagga ataaagaacc
     6241 cataaccaaa tgagtctaac ttttcaacca tttcttcatc tggatttaat aatgaaacaa
     6301 cgacacctgc taagaatgca cctaatatat tttctgcgcc aactccctct gctagggcta
     6361 ctaataatat tattaatgca aataccgcac gaataccgat ttgcgtcgta ccatccatca
     6421 atttttgtaa aaatgacatt cttttaaatt gaacacctaa aatatatgaa attgctgtga
     6481 aaacaactaa tatacctatt aaccatattg tactgccgcc ttgaccattg attgcgccat
     6541 agaccgttaa taaaatcata gttactaagt ccgcaagtac tgctactaat aggataaatt
     6601 gccctatagt tgttctcata atattcattt cttttaaagt tggaacaact acgcctaagg
     6661 aaatagttga aatgataatg accattaata acacatcatc cactaatcca agccatttaa
     6721 atacatacgc taaaagaatc gaaataatca taataaatgc aaatacagtt aacgctagat
     6781 taagatgccc tggaattgag gattcatcat cattttgtcc ttgacgtgcg cgtttatctt
     6841 ttttaaaagc tttaaaatca atttctaaac cacttaaaaa cattaaaaag ataaagccta
     6901 acgttgataa aatatttaga attgaatccc tttctactat atttagaaat gaatttccaa
     6961 tcacaatccc catcaaaatt tctgcaacaa caactggcaa gaaattaata tttaatcgat
     7021 tgacaattat tggagttaaa aacgctgcta aaacaacaat aactaaagat aaaaactcca
     7081 taactcctcc ttaaattaag taagccataa atgatgtagc taaaccaaaa taaatcaaca
     7141 tactaataat atcattaatt gttgtaataa atggtccact agccactgct ggatcgatat
     7201 ttaatttatt catcaataat ggaatcatcg aacctacaaa cgtccccacc gtcatcgcac
     7261 aagttaaact tcctgcaacg attaatgcta aaagtggctg atgatatatt gcaacaataa
     7321 ttgtaaataa tattgttgaa catacaacac ccgataatac accacttcct gcttctctta
     7381 atgcaattct aaatttactt tgctcattaa tttcccctgt cgtaatgtta cgaactgaaa
     7441 cggctaaaga ttgtgtacct gaatttcctg acataccact aataatagga ataaacgctg
     7501 cgagtagcgc tacattttct aatgtttttt cgaatctccc taaaattgtc gcagtaatca
     7561 ttcctaaaaa tgttaaaata atcaaccatg gtaaacgttt taatgctgtt ttaatgattg
     7621 aatcattagt cgaatcgata tctgataccc cggctaaacg agagtagtct tcactagcct
     7681 cttcatccat aacgtctaaa atatcatcaa tcgtgatgat accaagcaaa tgttcttggt
     7741 aatctataac aggtacagcc atgaaatcat agtctctcat aacttgagca acatcttctt
     7801 ggtcgtctgc tacattcaca ctaatgacac gttcattcat aatatcttca atataagcat
     7861 cattttcagc tacaattaaa tctcttagcg ataaaacacc tactaattta ccatcatcat
     7921 caacgacaaa tataacatat attgtttctg cgtctggtgc ttgcgctttg accaataata
     7981 atgcttcttt aacaggcgta tgcgctttaa gtgataaata ctccgtcgtc ataataccgc
     8041 cggccgtatc ctcatcatag tgaagtaatg ctttgatttc attcgcgtca tctttattca
     8101 tcaatgttaa aagactagca acttttggtt tagtcaactc atttaaaata tctactgcat
     8161 tgtcgtatga catttcttct aatatgtgac ttgcgtatgt cgcattcatc ttatcaaata
     8221 gcaactcata ttcatcgtca tcaatatcta attgatcaaa gaaatttgca acttcttcag
     8281 gtgataaata ttgaaaaatc ttttgtctat tttcatcggt agtatcttca aaatactcac
     8341 tttgttcata tgtgtgtaat gctagaaatt catctctaaa ttgatcaata tcatcatttt
     8401 ctaaatattg atctaataaa gtttggtcgt ataattcctc ttgaacacgc tctttttcat
     8461 ctgtgttcat tgacatactt acaccccctg tgtgaattag agttgattga ataatttaag
     8521 taattgctta taatcaattg taatataaat attgttctta ttgattggat gaacaaaata
     8581 tatttgcgta cattgcagta cttgaccatg aattttgtca tgaaaaccat catacaaaga
     8641 atctcccaca attggatgcc caatatgttg aaaatgtaca cgaatttgat gtgtacgtcc
     8701 cgtatgcaaa tgaactttgc ataaactgta tttatcattc tgatttatta cttcataaga
     8761 tgttttagcg tatttaccat ccgaggcaac ttctctagtt ataatcctat cctttgaccg
     8821 tctaatatta gcttcaataa taccagatgt atgggtttta ccatatacaa ggcaagtata
     8881 tatttttttc aagtttactt tagaaaataa atgatggata tgtccatatt tagcgaatat
     8941 cacaatacca gttgtattac gatctagacg cgtaacaata tgtgggttaa tattttcacc
     9001 atgttcctga caatgatata gtacttgttc gattaaactt tcatgaggat gttctctcga
     9061 aggcgtacaa ttttgattgt ttggtttagt aactatgatg ataaaagcat cttcatacaa
     9121 tacttctagc ttacgagcat aaggtattaa attaacactc ggtatttctc gcggtaaatg
     9181 aatttctaat atatcatttg tcattaattg cttacgcact gtgactggtt catcattaac
     9241 aattaaagcg ccattatttt taatggcgct cactgtcttc ttagaaaaat catgtcgtgc
     9301 taaaaaagtt ttaacagttt cttgttgtga tatatgatac ttaaatttca tcgttcttca
     9361 tcacttgata tgaaagaatc gtgcacacgt ttccagaatg ggaatggtct aaaacgtgca
     9421 aacctcactt tttcatttgc tacacggtat tgtattgaat taacattttt atgtttgata
     9481 ctaacatgat ctatcgtcat tctaatggta tcatgattaa ctggtgatat taaacatgta
     9541 tgatgctttg gtaatacaag tggtgatcct accgttctaa acacacgatt atttatcgag
     9601 gcaatttctg taatttgcat tgcttcaagt gaaggatgta tcagtgcgcc acctagcgct
     9661 ttgttataag ccgttgaacc cgaaggtgtt gatacacata atccatcgcc tctaaatcgc
     9721 tcaaagtgtt tccctcttaa gttaacatcc acaacaagtg ttgagccatt ttcagttttc
     9781 atcgttgctt catttaatgc taaatacctt gtttcatagc cgttgtcgtt gtatctcata
     9841 ataatttcaa gcaatggata ttcaatgacc tgaaactctg aattattaat ttcgatgatt
     9901 aatttttcaa cttcatgagg taaccaatcc gcataaaatc ctaaatgacc tgtatgaact
     9961 ccaacaaatg ccacttttga taacatgtgg ctatactgat ggaatgcttg tagtaatgta
    10021 ccgtcaccac caactgaaat aacaatttca ggattttcac tatcctcaat catgcgaaaa
    10081 tctttcatat agttcatcat tttatgcttt aaggcattag acttggagtc acctttagtt
    10141 aaaattgtat aacgcatgat ttataacacc tcgttaatca ttttcatgtt ttttagaacg
    10201 tttttgcgtg taatattttt gagcttcctg aatttcatct ttaatttcag acatctcttc
    10261 atcaagtaaa tacgctgctt ctgccgctct ttccaaacga tgttgaattt catccggata
    10321 agcaccatca tatttatatc gtaaagtatg ttcaatcgtt gcccagaaat tcattgctaa
    10381 tgtacgaatc tgaatctcag ccaatataaa tttttggcct tgtaatgttt caattggata
    10441 ttcaataatg acatgatacg agcggtaacc actttcttta gtgttacgaa tataatctcg
    10501 ttcttcaatt actttaaaat cttttctttg tcttaaaata ttgacgacaa catcaatatc
    10561 ttcaacaaat tggcacatca ttcttaaacc agcgatatcg tacatttctt ctcttaacct
    10621 atcaaatggt atttgtcgtt tgtttgcctt atctataata ctagcgattg gtttaacacg
    10681 accagtaaca aattctattg gcgacgcttg ttcaccaact tcatattgtt tgcgcatgcc
    10741 tttaagtttc actttcaact catcaaccgc ttgcttataa ggtgttaaga actgatccca
    10801 ttgattcata gtgcttcacc ccgctttatt caatgtcgaa tgtttcttca acggttgcaa
    10861 caaattcctt accataatta ttattccctt ttatgttgtc taaaatataa gtaagttctt
    10921 ggtggaattc agtcaattct agttcatcat tgataataat ttttttagtc atgttttcat
    10981 gcaacatcgt ccatgtttct tgtacgaaaa tgagataagc atacgcttta ccatcatcta
    11041 atgtatacgc aaaggcataa ttatcactat caacgagcat ttgtcctact tcagataatc
    11101 ctttaataga atcttctgta taacaataaa gtatgtcatc tttaatttta atttcattaa
    11161 tatataaacg catgcttaat cctcctctta ttccataaca ttttaacaca attcaataat
    11221 acaaaacact tacgaacact atttttaatg gtcaaattaa cattgataat agataaagga
    11281 aattactttt aaaattcaac tttgtcattc ataatatagt taagataaat tgaatttgat
    11341 acgaggtgta tacatatcat ggcaacaaat catgaaatag aatttaaaca aatgataact
    11401 gcatcaatct ataataaatt gcaagagaaa tattttaaag acagtgtgct ttttaagcaa
    11461 gtcaattact atattgatac acccgatttc aaattaaagg aacatcgctc tgcactacgc
    11521 ataagagtta aagacaatca atatgaaatg actttaaaga caccagcaaa ggttggtcta
    11581 ttagaataca actatatcgt cgacattaaa ccagagatga acttgattat ttcaaacgac
    11641 aatcttccag atgacattcg tcaaattata gttgaacagt ttggtgtaaa agatacaact
    11701 ttatcaatac ttggtgcatt aacgacatat cgccaagaaa ctaaatataa aggtgattta
    11761 ctggttttag ataaaagtga gtatttcgat actacggatt atgagttaga gttcgaagtt
    11821 aaagactata atcaaggttt acaaaagttc caatctttat taaacgaact aaacctcgaa
    11881 catcatcaac ctttaaataa agttcaacgt tttttcaaga aaaaagaaac actttcaaac
    11941 aatataaatt aaattggcaa atatgcatat ttctttcaaa tgtttaaaat catgttatat
    12001 tagttatata ttaacttaag ttcgtaacag taaatttttg aaaatgggtg attaaatgac
    12061 aacaacacca tatgacatca ttggtaaaga agcgttatac gatatgattc attattttta
    12121 cacccttgta gaaaaagatg aacgacttaa tcacctgttt ccaggagatt ttgcagaaac
    12181 aagtcgtaaa caaaaacaat ttttaacaca gtttttgggc ggtccaaaca tttataccga
    12241 agaacacgga catcctatgc taagaaaaag acatatagat tttacaatca ctgaatttga
    12301 aagagatgca tggttagaaa atatgcagac agcaattaat cgcgctgcgt ttccacaagg
    12361 tgttggagat tatttatttg agcgcttaag attaactgct aatcatatgg tgaattccta
    12421 aaattaattg taggtgaata aacatggctg gagaattacg aataatggaa aataagagtc
    12481 gtgaagatat caatctatca cctgtaagta aaatcgaaat ttattcattt ttcgatccat
    12541 ttagctccga ttgcttcaaa ttatcagcaa tcttatccaa attaagaatt gaatataatc
    12601 aatatatacg tatcagacat atattaaatc cttcgttaaa agtattaacg aaatgccaag
    12661 ctcaaagtac atccaacttt gataacatcg ccctagctta taaagcagct gagttacaag
    12721 gtcgtgtacg tgccgaacga tttatacatt taatgcaaaa tgaaatcata cctaaaagag
    12781 atattattac agaatcaatg atttgtgact gtattcaaaa tgcaggtatt gatttagaag
    12841 tatttaaaga cgacttacaa aaaagtaaac taaccgaaag cttgaaaatt gatttgcata
    12901 ttgcaagaga aatggaaatc gaacaagccc cttctctcgt tttctttagt gaagatgttc
    12961 atgaagaagg tttaaaagtc gaaggattat acccatatca catctatact tatataatta
    13021 atgaattgat gggtaaacct atcgaaaaga atcttcctcc taaattagaa acttatatac
    13081 agcaacaaca acttgtaacg atggaagaat tacttactat ttatgaatgg ccagaaaaac
    13141 ttttaaacaa agagttaaag aagttagcca ttcaacaaaa aattgaaaaa cttaaatatc
    13201 cagatggcga tttctggaaa tctaaaatgc ctaaaatcaa atcaaaataa ataaattaaa
    13261 gacctcgtac accataaatg taacgaggtc tttttactga ctcttaattt tgcaacatta
    13321 aattattaga acgtaatgtt acacatcgat ctaaatatat tagtgatatt tttcgaattt
    13381 aaccagttcg aaaattatac aaattataaa tttggtacat aatagacata tatgaatcgt
    13441 taatgtataa aaagtgcata tttaatacat ttttatacaa cttcacttcc ctcttattaa
    13501 aaaagaacat aaaaaaacgt cgtgtctgaa tacacgacgc taaacaaagg ggatgggaga
    13561 aatttttcac ttcaaacaaa ggggtatgtt tgttatgtga ttaatttcat gttcataata
    13621 taacatgcta tctctaccat ttcaaccttt taatagtact taatttaaat tgtcacaatc
    13681 tgttcataat gtaagcgaat acaatagtac taagctttca ttaatttttc aaaagcgttc
    13741 aatttttgtt caaaaacttc acaagcttgt tcaattggtt caggtgttgt catatctaca
    13801 ccagcatttt ttaatatctc aattggataa tttgagctac cttttttcaa gaattcatta
    13861 atatatctat ctactgctgg cttaccttct gttaaaattt gatgacttaa gctttgagct
    13921 gcactgtaac cagttgcgta ttgatataca taataattca tatagaagtg tggaatacgt
    13981 gaccattcct tactaatatc ttcatcagtt tctacagaat caccgaagta taatttattt
    14041 aatttggcat attcttcatt cattctagtt ggcgttaatg gttcacctgc ttcttcaatt
    14101 gcatgaattt tatgctcaaa ttctgcgaac attgtttgtc ggaataatgt agctctgaaa
    14161 cgttctaatt cttggtttaa taataataag cgtttttcat catcaagatg tttatccata
    14221 taatcactta aaagtgcttc gttacaagtt gatgcaactt cagcgacaaa aatagtgtag
    14281 tcactagaat ttgacggttg gaattttcta ctgaagtaac tatgtgctga atgcccaaat
    14341 tcatgaacta atgtgtataa gtctgaaata gtattagacc agttaagtag aataaatgga
    14401 ttagttaaat gtgcacctga tgaatagcca cctgaacgtt tacctttatt ctcatagaca
    14461 tcgacccaac gattgtttaa gccttcttta actacattta aatattcttc acccattggt
    14521 tctaatgctt ttaacatcca ctctttagct tcttcataag gcatttcaaa cttaatatct
    14581 ttaattaatg gtgtatataa atcatacatt ttcaagtcat ctaaacctag caattctttg
    14641 cgcaattcag tatatctatg tagcaatggt aaatatttat gtacagtttt tactagattg
    14701 tcatatacat tttctggaat atgattatta ctcaatgctt tttctcttgc agttttgtaa
    14761 ttgtgtgtac gagcattaaa tacatttttc ttcacttcac ctgctagcgt agcgccaagc
    14821 gtattattat gagcaccata tgctttatat acatttctaa aagcactttc tcttagtttg
    14881 cgatcatctg tttctaaata cttaataaat gtaccttgtg ttaacgggtg tgcatttcca
    14941 tctttatcta tcgcatcttc aaatactaaa tcagcgttgc taaacatacc gtatacattt
    15001 gatggcgttg ataacgcgtc ctgcgcttct gttaataact tttcagtttc agcatctaaa
    15061 atatgaggac gtttttcatt aatcaacttc aaatcaaatg cgaatttttg taatttatca
    15121 tatgaattta caaatgattg aattttatct tcatcaattt gtaaaatctc tggcactaag
    15181 aaactccatg ccgagctaaa tttaataatt aattgatgtg ctcttgactc cataccagta
    15241 tacttgtcgt tcgttgtatc ttggtcttgt tttaggtgcg cgtatacata tactttttct
    15301 aatttagtac ctaatgtatc ttctaattct aacgcattgt ataatgtctc agcactatca
    15361 ccaatgtgtc ctttaaattg ttcttctttg cctaactcat tttcaacttc tttaaatgca
    15421 gcctcaaaag cttcatcatc tttgaaaatt gttgttaagt cccatgtata ttcaggatat
    15481 ttacgttcct gttcttctct cgataattgt tgactcatat gtagtaaacc tcctaataaa
    15541 atataattga tataattttc tcattttaga acaaaatttg cacgtcatta cctttatctg
    15601 aacaaatatt taaataattg ttaataagtt tttctataat tgctgtttta ctttctagag
    15661 caacaataaa ttttttcagt ttaaaataat taagattgtc atttactaat ttgctttttc
    15721 cattctttaa ccataaatca acttgtaatt gccactcaat aggatgattt ttaatataaa
    15781 tttgctctgg aaaaatataa ccataattgt gcactacttc ttgatcagtt aactgtaatt
    15841 gatacattgc acttaaagtg ggttctaaaa cagaattttg ccagcgacaa tatttgatat
    15901 attgattaat tgcgaacttt gataatttat aaacagcatg ataatcacaa ggcgcctcat
    15961 taaacaactc actaattttg gcatttcttt tttcagcgac atatttgtgc ccgcctaaat
    16021 attgtatttg ttgaaacatc attatttgtt ttttctctaa attgaacgta tgaagttttc
    16081 gagtgaatgg attaataaac atactttgaa aatgattgaa cttcacttta ttatgacaat
    16141 aatcaacatc atttaataac caacttactt tatatcccat tttctttaaa ccttccgttc
    16201 gttgaagaat ctgtttataa ggaattggcg aatactgtag ctcaataaca tatttattat
    16261 taatcaaaat atccggaatt tgttttattt cttttaaaat ggctctatct ctactttaca
    16321 tccttgttgc tgtatcatct gtgctaaaat caatttactt ttataatgtt taatcgtttc
    16381 attattaaaa catttttgtt ccgctaaatg tttatgtgca aaatgactaa ttactttgag
    16441 cccacgcttt aaaataactt gcttgccaca caccggacaa aaatattgtg tctttctcaa
    16501 tgcagtagtt gctaatacgc gttccttttc ttcatttaaa gctactaaca tatcttcacc
    16561 tcaaaatatt acacgtaagt ttaactacta ttactttttc agcttttgaa atcaataaaa
    16621 aaagagactc caaagagtct cctttcatag tgcgttgctt aattaatatc gttattcagt
    16681 tgtctctgga aaataacgtc gaacttgagc tgttacgtta tgactcataa ttattttagc
    16741 atagtcattt aaataaactt ctgttctgtc tgttggataa gcaaattcaa gcaattgact
    16801 gtaactatca ttaatgactt cttgatcaac atgactatca aaatatacag cataataata
    16861 agtaccatca accatatata acaaatcttc aaactctgta gttattggat tgctatgata
    16921 tgcataatta ataacatctt ctaaatcgtt aaatttcaca atgattgttc ttgtattttt
    16981 acgtgctgaa gacttttgac gtttagaacc ttgagcttct ttttcttttg ttcgttgctc
    17041 gaataattct tctaattgat cttcaccttc taatgtttga gctaacaatt cttgaacttg
    17101 ttcatcaaat tgatcagttg catcatcatc agacatattc atcatatctt catttttaga
    17161 tttagaaatt gtgacttcga cacctttttc aaaggcatgt acttgaatcc ataatggacc
    17221 ttctacaaca aaatcttctt cttcgttaat ttcatccatc attgaccaaa agaattcttc
    17281 gccacgtttg cgatttgtcc ataaatcttc acgactaaat ccacgggcct cgatatcgct
    17341 atatgttata aacaatttta cagttgtatc atctactcgt tctattctca tatcatctca
    17401 ctccttacag tcgatgaata gtatccacat tgtattaaag catcaaacaa aatacaattt
    17461 atttgtttga tttctttctc taaaatattt tggtaactta taataacata ttcgttgtag
    17521 caa
//
LOCUS       DALAGD010000067        17009 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.068, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000067 DALAGD010000000
VERSION     DALAGD010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17009
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.068"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            1..1191
                     /locus_tag="O2701_001822"
                     /pseudo
     CDS             1..1191
                     /locus_tag="O2701_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499232.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            1337..1696
                     /gene="sarX"
                     /locus_tag="O2701_001823"
     CDS             1337..1696
                     /gene="sarX"
                     /locus_tag="O2701_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarX"
                     /protein_id="HCZ4293709.1"
                     /translation="MNTEKLETLLGFYKQYKALSEYIDKKYKLSLNDLAVLDLTMKHC
                     KDEKVLMQSFLKTAMDELDLSRTKLLVSIRRLIEKERLSKVRSSKDERKIYIYLNNDD
                     ISKFNALFEDVEQFLNI"
     gene            1886..2602
                     /locus_tag="O2701_001824"
     CDS             1886..2602
                     /locus_tag="O2701_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCZ4293710.1"
                     /translation="MGRKWNNIKEKKAQKDKNTSRIYAKFGKEIYVAAKSGEPNPESN
                     QALRLVLERAKTYSVPNHIIEKAIDKAKGAGDENFDHLRYEGFGPSGSMLIVDALTNN
                     VNRTASDVRAAFGKNGGNMGVSGSVAYMFDHVATFGIEGKSVDEILETLMEQDVDVND
                     VIDDNGLTIVYAEPDQFAVVQDALRAAGVEEFKVAEFEMLPQTDIELSEADQVTFEKL
                     IDALEDLEDVQNVFHNVDLK"
     gene            2602..3075
                     /locus_tag="O2701_001825"
     CDS             2602..3075
                     /locus_tag="O2701_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCZ4293711.1"
                     /translation="MKSAEQWIDELQLESHPEGGFYRETIREVLKDGRRAPFSSIYFL
                     LTDDNISHFHRIDADEVWYYHAGDSLTIHMINPDGEYTTATLGTDIQNGDVLQYVVPK
                     GTIFASSIEISNTFSLVGCMCQPAFEFKQFELFKQSELITQYPHLKSVIEKYALK"
     gene            3167..3283
                     /locus_tag="O2701_001826"
     CDS             3167..3283
                     /locus_tag="O2701_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293712.1"
                     /translation="MLQLFLMKIALNDFENDKSVLFIFLKNHFYEEMCVLSN"
     gene            3428..4072
                     /locus_tag="O2701_001827"
     CDS             3428..4072
                     /locus_tag="O2701_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCZ4293713.1"
                     /translation="MKVKYIDKRHWRRLIDREYTEVKVNNNRFKGIIGLVTMKKVRDP
                     LEVTVVGQNIIVADDNYKWLQILPEKKRYSITVMFDNKGNPLEYYFDINIKNITQKGN
                     ARTVDLCLDVLALPSGEYELVDEDDLMFALESEQITKKQFHEAYMIAHQIMAELENDF
                     KGFQKKIMYCFNKINAKAQKNHQKPQNKTNIEKSKQIKPKQYNQTKNHQQQKKN"
     gene            4188..5054
                     /locus_tag="O2701_001828"
     CDS             4188..5054
                     /locus_tag="O2701_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCZ4293714.1"
                     /translation="MKIEDYRLLITLDETKTLRKAAEILYISQPAVTQRLKAIENAFG
                     VDIFIRTKKQLITTTEGTMIIEHARDMLKRERLFFDKMQAHIGEVNGTISIGCSSLIG
                     QTLLPEVLSLYNAQFPNVEIQVQVGSTEQIKANHRDYHVMITRGNKVMNLANTHLFND
                     DHYFIFPKNRRDDVTKLPFIEFQADPIYINQIKEWYNDNLEQDYHATITVDQVATCKE
                     MLISGVGVTILPEIMMKNISKEQFEFEKVEIDNEPLIRSTFMSYDPSMLQLPQVDSFV
                     NLMASFVEQPKA"
     gene            5324..6544
                     /locus_tag="O2701_001829"
     CDS             5324..6544
                     /locus_tag="O2701_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HCZ4293715.1"
                     /translation="MDMFAALLQIKNYKLFVANMFLLGMGIAVTVPYLVLFATKDLGM
                     TTNQYGLLLASAAISQFTVNSIIARFSDTHHFNRKIIIILALLMGALGFSIYFFVDTI
                     WLFILLYAIFQGLFAPAMPQLYASARESINVSSSKDRAQFANTVLRSMFSLGFLFGPF
                     IGAQLIGLKGYAGLFGGTISIILFTLVLQVFFYKDLNIKHPISTQQHVEKIAPNMFKD
                     KTLLLPFIAFILLHIGQWMYTMNMPLFVTDYLKENEQHVGYLASLCAGLEVPFMIILG
                     VLSSRLQTRTLLIYGAIFGGLFYFSIGVFKNFYMMLAGQVFLAIFLAVLLGIGISYFQ
                     DILPDFPGYASTLFSNAMVIGQLGGNLLGGAMSHWVGLENVFFVSAASIMLGMILIFF
                     TKNQKITKEDVIST"
     gene            6541..7029
                     /locus_tag="O2701_001830"
     CDS             6541..7029
                     /locus_tag="O2701_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HCZ4293716.1"
                     /translation="MTIILWLLIIAAFMLAFVGLIKPIIPSVLVLWVGFLIYQFGFHN
                     QHLSWVFYVSMALLTILILCADFLANKYFVNRFGGSKFGEYAALIGVVIGCFVLPPFG
                     IIIIPFILVFIVELIQGYSFERAVKVSIGSIVAFLTSSIAQAIIMFIMIVWFFIDALL
                     IN"
     gene            complement(7128..7817)
                     /locus_tag="O2701_001831"
     CDS             complement(7128..7817)
                     /locus_tag="O2701_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 family protein"
                     /protein_id="HCZ4293717.1"
                     /translation="MKSTAQLTKENNVKSLRLSNTDREIFENYMTYMRSDFRVNPHDT
                     ELIINRILKQLLSAEQHGLLALDFFNHDPKAHAIKELKAMPNETFKNIFKYIYQHIVL
                     LIGIVSFLKGFLGFFMEKNGSNLYFVSFPFSVVVGFFIVFLFIWFSFKTIQLQCFNNS
                     NWIWIFTYLAIILLIVGFFYVFFIPQSLLAFGPYIQVSNWVFIIFSFIVMPIGLRIER
                     NISKKHSHTFL"
     gene            7944..8387
                     /locus_tag="O2701_001832"
     CDS             7944..8387
                     /locus_tag="O2701_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4293718.1"
                     /translation="MRTLNKDEHNYIKQIANIHETLLSQVESNYKCTKLSIALRYEMI
                     CSRLEHTNDKIYIYENEGQLIAFIWGHFSNEKSMVNIELLYVEPQFRKLGIATQLKIA
                     LEKWAKTMNAKRISNTIHKNNLPMISLNKDLGYQVSHVKMYKDID"
     gene            8454..8849
                     /locus_tag="O2701_001833"
     CDS             8454..8849
                     /locus_tag="O2701_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293719.1"
                     /translation="MKKLIISIMAVMLFLTGCGKSQEKATLEKDIDNLQKENKELKDK
                     KEKLQQEKEKLADKQKDLEKEVKDLKPSKEDNKDDKKDEDKNKDKDKDKEASQDKQSK
                     DQTKSSDKDNHKKPTSADKDQKANDKHQS"
     gene            8987..9286
                     /locus_tag="O2701_001834"
     CDS             8987..9286
                     /locus_tag="O2701_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293720.1"
                     /translation="MHEQDFRILEGQDITLPELGRELENITGHTIADSTGEIKRVIAH
                     LPNFESDTDTFVATYRLNHQQDFIDATFTALKSDRARLKEVPVHVELISYISKSK"
     gene            9366..9908
                     /locus_tag="O2701_001835"
     CDS             9366..9908
                     /locus_tag="O2701_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4293721.1"
                     /translation="MIYCETERLILRDWHEDDLLPFQKMNANYDVRKYFPSLLSYRRS
                     ELDMRTMDAVIKDYGIGLFAVEDKESHQWIGFIGLNYIPETSDYPFKELPLYEIGWRL
                     LPEFWGKGLATEGAKATLKLAEEHQIYDVYSFTAEANKASQRVMEKIGMTVYDHFELP
                     NLSKYHLLKRQVRYYINLRK"
     gene            complement(10004..10570)
                     /locus_tag="O2701_001836"
     CDS             complement(10004..10570)
                     /locus_tag="O2701_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HCZ4293722.1"
                     /translation="MKRIAVYCGASKGHDPSYVQKAYDLGKYFAEQGYELVFGAGSIG
                     IMGAIQDGVLDHGGKAIGVMPKMLDEHEITSQRLTELILVDSMHERKNKMAELADAFV
                     MAPGGAGSLEEFFEMYSWAQIGIHEKPIAIYNINGFFNPLQTMIDHMIEEGFIDPKYR
                     ALAPLCDTKESLIESILNFKPLGTRSYD"
     gene            complement(10572..11030)
                     /locus_tag="O2701_001837"
     CDS             complement(10572..11030)
                     /locus_tag="O2701_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCZ4293723.1"
                     /translation="MTHIIIDGDACPVVDSIIDLTTETGIFVTIIRSFSHFSNQLYPP
                     HVSTLYVDDGPDAVDYKIVQLSTKDDIVVTQDYGLASLLVDKVLIVMHHNGKIYNSKN
                     IQQLLDKRYMNAQIRKQGGRHKGPPPFTKQDQKVFEQSLLKVIHRIKELD"
     gene            complement(11033..11716)
                     /locus_tag="O2701_001838"
     CDS             complement(11033..11716)
                     /locus_tag="O2701_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293724.1"
                     /translation="MDKKKVIKFMINVLPIVLVPLIVERKRIKQHPDVQKVTDATSKV
                     ASKTSAAISNTASDVKEYVGDKKQDFENKRELKKFAREHDPAYIEKKGEKLAKQNRKD
                     ADKMNKILQKNIEKRHKEEQKAREKNEIQRIKDMKKSQKYEEKAGLTPNKLDEKTEKK
                     GDKLAEKNRKEIAKMNKKLQKNIEKRHKEEQKRQQEADKARIKSFKKYKDYVAKSASQ
                     QNKENNTEA"
     gene            complement(11888..12763)
                     /locus_tag="O2701_001839"
     CDS             complement(11888..12763)
                     /locus_tag="O2701_001839"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCZ4293725.1"
                     /translation="MFIIELIKGIILGVVEGLTEFAPVSSTGHMILVDDMWLKSSEFL
                     GSQSAFTFKIVIQLGSVFAAAWVFRERFLEILHIGKHKHVEGDNDQQRRSKPRRLNLL
                     HVLVGMVPAGILGLLFDDFIEEHLFSVPTVMIGLFVGAIYMIIADKYSAKVKNPQTVD
                     QISYFQAFVIGISQAVAMWPGFSRSGSTISTGVLMKLNHKAASDFTFIMAVPIMLAAS
                     GLSLLKHYQDIQIADIPFYILGFLAAFTVGLIAIKTFLHLINKIKLIPFAIYRIVLVI
                     FIAILYFGFGIGKGI"
     gene            12976..14607
                     /locus_tag="O2701_001840"
     CDS             12976..14607
                     /locus_tag="O2701_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HCZ4293726.1"
                     /translation="MKKLTTILFQYKIFPVLMFLVSTGLGIIVITQNILIADFLAKII
                     RHQFQGLWIVLFILLGVLLLRATVQFLNQWLGDTLAFKVKHMLRQRVIYKNNGHPIGE
                     QMTILTENIDGLAPFYKSYLPQVFKSMMVPLIIIIAMFFIHFNTALIMLITAPFIPLF
                     YIIFGLKTRDESKDQMTYLNQFSQRFLNIAKGLVTLKLFNRTEQTEKHIYDDSTQFRT
                     LTMRILRSAFLSGLMLEFISMLGIGLVALEATLSLVVFHNIDFKTAAIAIILAPEFYN
                     AIKDLGQAFHTGKQSEGASDVVFEFLEQPNYNNEFLLKYEENQKPFIQLTDISFRYDD
                     SDRLVLNDLNLEIFKGDQIALVGPSGAGKSTLTHLIAGVYQPTIGTISTNQRDLNIGI
                     LSQQPYIFSASIKENITMFKDIENNTIEEVLDEVGLLDKVQSFTKGINTIIGEGGEML
                     SGGQMRRIELCRLLVMKPDLVIFDEPATGLDIQTEHMIQNVLFQHFKDTTMIVIAHRD
                     NTIRHLQRRLYIENGRLIADDRNISVNITENGDDL"
     gene            14604..16277
                     /locus_tag="O2701_001841"
     CDS             14604..16277
                     /locus_tag="O2701_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding/permease
                     protein"
                     /protein_id="HCZ4293727.1"
                     /translation="MKTRLKFQVDKDLLLAIVVGVCGSLVALAMFFLSGYMVTQSALG
                     APLYALMILVVTVKLFGFLRAITRYVERLISHKATFTMLRDIRVQFFGKLVNVIPNVY
                     RKLSSSDLISRMISRVEALQNIYLRVYYPPVVIGLTALVTVIVLAFISIGHALLIMVS
                     MLFTLLIVPWLSSKKARTLKKHAANEQARFLNHFYDYKAGMDELRRFNQINHYRDNLM
                     AKLNHFDKLQLKEQRFLTIYDFILNIIAMLSIFGSLVLGLIQINAGQLNIIYMTSIVL
                     MVLTLFEQAVPMTNVAYYKADTDQALHDINEVISVPSTNGKKRLNDKYDATNIYEVKD
                     ASFKYWNQQTYVLSDINFNVNRGEKIAIVGPSGSGKSTLLQIMAGLYQLDSGSVRFEN
                     MDMFEIDDKDKFESLNVLLQSQQLFDGTIRQNLFTDEKDEAVQAIFKQLDLEHLALER
                     QIDLDGHTLSGGEIQRLAITRMLLKDTASTWILDEPTTALDKQNSLKVMDLIEAHAET
                     LIVATHDLTLLSRFETIIVMINGKIVEKGNYQQLLANQGALWNMIQYNA"
     gene            complement(16404..16847)
                     /gene="mgrA"
                     /locus_tag="O2701_001842"
     CDS             complement(16404..16847)
                     /gene="mgrA"
                     /locus_tag="O2701_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MgrA"
                     /protein_id="HCZ4293728.1"
                     /translation="MSDQHNLKEQLCFSLYNAQRQVNRYYSNKVFKKYNLTYPQFLVL
                     TILWDESPVNVKKVVTELALDTGTVSPLLKRMEQVDLIKRERSEVDQREVFIHLTDKS
                     ETIRPELSNASDKVASASSLSQDEVKELNRLLGKVIHAFDETKEK"
BASE COUNT         5983 a         2339 c         2916 g         5771 t
ORIGIN      
        1 agttttctta gaaggaatct ttaaaacctt attgaataag aacagtcaag ttgtcttttt
       61 catcatgcca tcgattctaa aaagtaaaaa taccatgtcc gaagaagaaa aattcacaat
      121 cattaaaaca ataattgaaa gtgatctaaa gatagcattt aatataaata atattgaaac
      181 aacttatttt gttgaagaag cttttatgag tgttttcaga caaatatctc caaacgaatt
      241 aagtaatcat aataattacg aagtgcattt tgtttttgat ttatcattga tggaaattag
      301 aacaatttat cgaatgatat taaaattaca taacatcatg ttgaatgtga aattaggatt
      361 gaacattacc tgtttatttg aaaaaccttc agtttttaaa tcactagtat cacaaataaa
      421 gcgacttaaa ttcgattcgt taataataga taatgcaaat ttaagtagcc cttatttgat
      481 gggggaaagt gatgagttac tattgaaaaa tattttgcat tttaaaaatt taaaacaagt
      541 aattaatgaa ttggatattg aacaagaaaa gctgattttt ctaaatgttg aaaatcataa
      601 actgcttaat aataaagaac gagatctaag taatagtgct ccattaattt ataagacatt
      661 aagtgcgctg tatcacaact ttgatggctt tggattaaac atttttgaca atcatcaagc
      721 atttaatgcg atgcatctat atgacaaaaa tggatttaaa acaacactag gtcttatatt
      781 agaaaagttt atcgaatatg tctcgaaacc aaaatacgaa aacagttatt attctatttt
      841 tgatatagag aattattatt gtcttgttat ttatgattgg cgagtgatag agagcgagac
      901 aattatgagt aattttgagg atagtcaagt ttatataaat tttaaaaaca atgttttaaa
      961 cgataaatat ctaattgtaa tagaaacatt ggacaaaata gtggcaacat taatcatttg
     1021 atttctaaag aattaagaga taaatatgaa tggaacccta gtttactatc taaaattgac
     1081 aactacctta aaccagcaat agagattaaa gagcataatt ttagtgataa ttccttgaat
     1141 attaacgtta cttttaatgc gttatacata attaaaatag gaaaaaaata acaccttgat
     1201 atgtattgca aaatttaatt tgcatttgtt ggtaattttg acaacgtatt aaaaacaaat
     1261 gagaaagaca atggctgaaa aatttaagat aataatgaca gaagcattgt ctttatatat
     1321 ttgggggtgc aacattttga atactgagaa attagaaaca ttgcttggct tctataaaca
     1381 atataaagca ttatctgaat atattgataa aaaatataag ttgtcgctaa atgatttagc
     1441 agtcttagat ttaacgatga agcattgcaa agatgaaaaa gtacttatgc aatcattttt
     1501 aaaaactgca atggatgagc tagatttaag taggacaaaa ttattagttt ctataagaag
     1561 actaattgaa aaagaaagac ttagtaaagt tagatcatct aaagatgagc gtaaaattta
     1621 tatttattta aataatgatg atatatctaa atttaatgct ttatttgaag atgtagaaca
     1681 atttttaaat atttaattga aattgagtgt cgaaagcata gaatttgctt atcggcacat
     1741 ttttaattta tacatatttt aaaactaagt aacagtttga agaaatcgta gttcaataat
     1801 gttaattgtg aaaatgtata taaacataaa aaaatcatgt ataatatatg ttgttaatta
     1861 aacagttcga aagcgagatg acattatggg acgtaaatgg aataacatta aagaaaaaaa
     1921 ggcccaaaaa gataaaaaca caagtagaat atatgcgaaa tttggtaagg agatttatgt
     1981 tgcagcaaaa tctggtgaac ccaatccaga atctaaccaa gctttaaggt tggtgcttga
     2041 acgcgctaag acatattcag tgccgaatca tattattgaa aaagcaatag ataaagctaa
     2101 gggtgctgga gacgaaaact ttgatcacct aagatatgaa ggatttggcc caagcggatc
     2161 aatgctaatt gttgatgcgt taacaaataa tgtaaatcgt actgcctctg atgtgcgagc
     2221 tgcttttggt aaaaacggcg gtaatatggg tgtatctgga tcagttgctt atatgtttga
     2281 tcatgtggca acatttggta ttgaaggaaa gtctgttgac gaaatacttg aaacattaat
     2341 ggaacaagat gtagatgtaa atgatgtgat tgacgataat ggattgacaa tagtctatgc
     2401 tgaaccagat caatttgcag tcgttcaaga tgcgcttcgt gcagcaggtg ttgaagaatt
     2461 taaagttgct gaatttgaaa tgttacctca aacagatatt gaactttctg aagcggacca
     2521 agtaacattt gaaaaattaa tcgatgcatt agaagattta gaagatgtac aaaacgtatt
     2581 ccataatgtg gatttgaaat aatgaaatca gcagaacaat ggattgatga attgcaactt
     2641 gaatcacatc ctgaaggtgg tttctataga gagacaattc gagaagtatt gaaagatgga
     2701 cgcagagcgc cgtttagtag tatttatttt ttacttacag atgacaatat ttcgcatttt
     2761 catcgaattg atgctgatga agtatggtac tatcatgctg gcgattctct aacaattcat
     2821 atgataaatc cggatgggga atatacgact gcaacattgg gtactgatat ccaaaatgga
     2881 gatgtattgc aatatgtagt gcctaaagga acaatttttg cttcttcaat cgaaatatca
     2941 aatactttta gtttagtagg ttgtatgtgt caaccggcat ttgagtttaa gcagtttgaa
     3001 ttgtttaagc aatctgaatt aattacgcaa tatccgcatc ttaaatcagt aatcgaaaaa
     3061 tatgctttaa aataaaagtg atcaatgaag tggtttgaag gttgttaata aacctttgag
     3121 tcacttcatt tttatatgta ttcttgattg aatcagaata gatttgatgc ttcagctgtt
     3181 tttaatgaaa atagcattaa atgattttga aaacgataag agtgtgttat ttatattttt
     3241 gaaaaatcac ttttatgaag aaatgtgtgt actatctaat taatattgtc tattttaagt
     3301 aaattgaagc gagttgtagt aaaataataa taaatatttt catatgatta acaaaaacta
     3361 taaaactgta tcatgacatg acatcattta tgtagtttta tataataatg acaaggagtt
     3421 ggtgactgtg aaagtaaagt atatagataa acgtcactgg cgtcgcctaa ttgataggga
     3481 atacacagag gtaaaagtta ataataatag gtttaagggt attataggct tagtcacgat
     3541 gaaaaaggtt cgtgatcctt tagaggtgac ggtagttgga caaaatatca ttgtcgcaga
     3601 tgacaattat aaatggttgc aaatactacc tgaaaagaaa cgttatagta taactgtaat
     3661 gtttgataat aaaggcaatc cattagaata ttattttgat ataaatatca aaaatataac
     3721 gcaaaaaggt aatgcgcgta cagtagattt atgtttagat gttttagcat taccaagtgg
     3781 tgaatatgag ttggtagatg aagatgattt aatgtttgca ttagaaagtg agcaaattac
     3841 aaaaaagcaa tttcatgaag catatatgat tgcacatcaa attatggcag agttagaaaa
     3901 tgattttaaa ggattccaaa agaaaatcat gtactgcttt aataaaatta atgcaaaggc
     3961 tcaaaaaaat catcaaaagc cacaaaataa aactaatatt gaaaaaagca aacaaataaa
     4021 gcctaagcaa tataatcaaa ctaaaaatca ccaacaacaa aagaaaaact aagtaattca
     4081 agctgcagcc ataccaataa aattggtgaa attattcgtt agtaatgaac tcatgaatgc
     4141 ttgtagccat gaaagttcaa taattgaata atttatgggg ggaaattatg aagattgaag
     4201 actatcgttt actaataaca ttagacgaaa cgaaaacgtt acgtaaagcg gctgaaattt
     4261 tatatatatc tcaacctgct gttacacaaa gactaaaagc tattgaaaat gcttttggag
     4321 tagatatttt tatcagaaca aaaaaacaat tgattacaac aactgaagga acaatgatta
     4381 ttgagcatgc tcgtgacatg ttgaaaagag agcgattatt ttttgacaaa atgcaggcac
     4441 atattggtga agtgaatgga acaatatcaa tcgggtgttc ttctttgatt ggacaaacct
     4501 tacttcctga agttttgagc ctatataatg cccaatttcc taatgttgaa atacaagtgc
     4561 aagttggttc aactgaacaa attaaagcaa atcatagaga ttatcatgtt atgataactc
     4621 gtggaaataa ggtaatgaat ttagctaaca cacatttatt taatgatgat cattatttta
     4681 tttttccaaa aaatagacga gatgatgtta caaagttacc atttatagag tttcaagctg
     4741 atccgattta tataaatcaa ataaaagaat ggtataacga taatttagaa caagattacc
     4801 atgcaactat tacagtggat caagtagcaa cttgcaaaga aatgttgatt agtggtgtag
     4861 gtgttacaat cttgccagaa attatgatga aaaatatcag caaagaacaa tttgagtttg
     4921 aaaaagtaga aattgataat gaaccgctga ttcgttcgac atttatgagt tatgatccga
     4981 gcatgttgca attgccacaa gttgattctt ttgtaaatct catggcgagc tttgttgaac
     5041 aaccaaaggc gtagttttag actaatttaa ggtttgtatt taattttaaa ctattcggtt
     5101 aaattgaacg tagttggttg ctaatgcacc aacagcaaaa gagcccctaa ttaataaatt
     5161 aaaaggggac aaaggaatac agttggttgc taatgcacca actgcataag agcccctaat
     5221 taataaatta aaaggggaca aaggaataca gttggatgct aacgcaccaa ctgcataaga
     5281 gcccctaatt aataaattaa aaggggctct aagaagcggg gttatggata tgtttgcagc
     5341 gttattacaa ataaagaatt ataaactctt tgttgctaat atgtttctac taggtatggg
     5401 tattgcggtt acggtcccat atcttgttct ttttgcaact aaagatttag gtatgacaac
     5461 aaatcagtat ggattacttc tagcatctgc agcgattagc cagtttacag taaattcaat
     5521 tattgctaga ttttcggata cgcatcactt taatagaaaa attattatta ttctcgcatt
     5581 attaatgggt gcgcttggtt tttcaatata cttttttgta gatacaatct ggttattcat
     5641 attactatat gcgattttcc aaggattatt tgcaccagca atgccccaac tttacgcatc
     5701 tgctagagaa tctatcaatg tttcaagctc taaagataga gctcaatttg ccaacacagt
     5761 attacgttca atgttctcat tgggcttttt atttggtcca tttattggtg cccaattaat
     5821 cggattaaaa ggctatgctg gattgtttgg tggaacaata agtatcattt tatttacttt
     5881 agtacttcaa gtgtttttct ataaggattt aaacattaaa caccctatta gtacgcaaca
     5941 acatgttgaa aaaattgctc ctaatatgtt taaagacaaa acgcttttat taccatttat
     6001 tgcatttatt ttattacaca ttggacaatg gatgtatacg atgaatatgc ctttatttgt
     6061 tactgattat ttaaaagaaa atgaacaaca tgtcggttat ttagctagtt tatgtgctgg
     6121 tttagaagtg ccatttatga tcattcttgg cgttttatca tctagattac agactcgaac
     6181 attgttgatt tatggagcga tttttggtgg tttattctac ttcagcattg gggtatttaa
     6241 aaacttctat atgatgttag caggacaggt gtttttagct attttcttag cggttctttt
     6301 aggaattggt attagttatt tccaagatat cttaccagat tttccaggat acgcttcaac
     6361 actattttct aatgcaatgg ttattggaca gttaggcggt aacctattag gtggtgctat
     6421 gagtcactgg gtaggtttgg aaaatgtatt ttttgtatca gcagcatcaa tcatgttagg
     6481 tatgatactt atattcttta ctaaaaatca aaaaattaca aaagaggatg tgatatcaac
     6541 atgacaatta ttttatggct acttatcatc gctgccttca tgttagcatt tgttgggttg
     6601 attaagccga ttattccttc tgttttagta ttatgggttg gctttttaat ctatcaattt
     6661 ggctttcata atcagcattt atcatgggtg ttttatgtat ctatggcatt gctaacaata
     6721 ttaattttat gtgccgactt tttagctaat aaatattttg tgaatcgctt cggtggttct
     6781 aagtttggag agtatgcagc tttaattggt gtggttattg gatgttttgt tttaccgcca
     6841 tttggaatta ttattatacc ttttattttg gtattcatag ttgaattaat acaaggctat
     6901 tcatttgaaa gagcagttaa agtaagtata ggttcaatcg tagcattttt aacaagtagt
     6961 atagctcaag caatcattat gtttataatg attgtatggt tctttataga tgctttattg
     7021 attaattaat aaaaagctta ttgcaaaata tgtttttcgg taactgtaat ttagtgattt
     7081 tatcattaac agtaccaaat tcgcatatga tgtaataagc tttttgttta taaaaatgta
     7141 tgagaatgtt ttttcgaaat atttctttca atgcgtaatc caataggcat aactataaat
     7201 gaaaatataa taaataccca attgcttacc tgaatatatg gtccaaaggc aagtaatgat
     7261 tgtggaataa agaatacata gaaaaatcct acaattagta gaatgattgc taaataggtg
     7321 aatatccata tccaatttga attgttaaaa cactgcagtt gtattgtttt aaaactaaac
     7381 catataaata aaaagacaat aaagaatcca acgactactg aaaacgggaa tgaaacaaaa
     7441 tataaattac ttccattttt ttccatgaaa aatcctaaaa atcctttgag aaaactaaca
     7501 atcccaatta atagaacgat gtgttgatag atatatttaa aaatattttt aaatgtttca
     7561 ttaggcatcg cttttagttc ttttattgca tgtgcttttg ggtcgtgatt gaaaaaatct
     7621 aaggctaata aaccatgttg ttctgcgctt aataattgtt tgagtatacg gttaataatt
     7681 aactctgtat catgagggtt gacgcgaaag tcagagcgca tataagtcat ataattctcg
     7741 aagatttctc tatcagtatt gcttaatctt aatgatttaa cattattttc ttttgttaat
     7801 tgcgcagtac ttttcattgt tacttaagcg ctcctttaaa aatgtttaat tccaaattaa
     7861 aatggaaatg attttatagt attaataagg tcaatcatat catattaaac gcataaatat
     7921 aacgattaat attggagagg aaaatgagga cacttaataa agatgaacat aattatatca
     7981 agcaaatagc taatatacat gagacattat tgtcgcaagt agaatccaac tataaatgta
     8041 ctaaactgag tattgctctt aggtacgaga tgatatgttc aagattagaa catacaaatg
     8101 ataaaattta tatatatgaa aatgaaggtc aattaatagc gtttatttgg ggacatttta
     8161 gtaatgaaaa aagtatggtt aacattgaac tgctatatgt tgaaccacaa tttcgcaaac
     8221 tgggaatagc tacgcaactg aagattgcgc ttgaaaaatg ggcaaaaact atgaatgcaa
     8281 agcgaataag caatacaatt cataaaaata atttgccaat gatatctttg aataaagatt
     8341 taggttatca agtgagtcat gtgaaaatgt ataaagatat tgattagaat taggattatg
     8401 ttgctaattc atgttaaaat taaaaaagat ttaatgacgt taaggagttt tatatgaaga
     8461 aattaatcat cagtattatg gcggtcatgc tatttttaac aggttgtggt aaaagtcaag
     8521 agaaagccac tctggaaaag gatatcgata atttacaaaa agaaaataaa gaattaaaag
     8581 acaaaaaaga aaagcttcaa caagaaaaag aaaaattagc agataagcaa aaagaccttg
     8641 aaaaagaagt gaaagattta aaaccttcaa aagaagataa caaggatgat aaaaaagacg
     8701 aagacaaaaa taaagacaaa gataaagata aagaggcatc acaagataag caatcaaaag
     8761 atcaaactaa gtcatcggat aaagataatc acaaaaagcc tacatcagca gataaagatc
     8821 aaaaagctaa tgacaaacac caatcataat cgaattgctt acttgttata gatgaaaggt
     8881 acagcgtttt aaaccttatt ttaagggtat gtattaatta aaatgtggtc atgattgaaa
     8941 cagaacgtaa aaatagacaa cgtaattaat aaaggagaga aacggcatgc atgaacaaga
     9001 ttttagaatt ttagagggtc aagatattac tttgccagaa ttaggtagag aattagaaaa
     9061 tattacagga catacgattg ctgattctac tggcgaaatt aagcgtgtaa ttgcacattt
     9121 accaaacttt gagtccgata cagatacttt tgttgctaca tatcgtttaa accatcaaca
     9181 agattttata gatgcaactt ttactgcgct gaaatcagat agagcacgtt taaaagaagt
     9241 gccagttcat gttgaactta taagttatat ttctaaatca aaataaactg ctatctaaaa
     9301 cgcaaagttg atcaaaatat cgattttgtg ttttttattg agaaattata taggagtgtc
     9361 aatcgatgat ttattgtgaa acagagcgtt taatattaag agactggcat gaagatgatc
     9421 tgttaccttt tcaaaaaatg aatgcgaatt atgacgtacg taaatatttt ccaagtttat
     9481 tgagttatcg tcgttcagaa ttagatatga gaactatgga tgcggttatt aaagattatg
     9541 gcattggatt atttgctgta gaagataaag agtcccatca atggataggc tttataggtt
     9601 tgaattatat tccagaaaca agcgattatc catttaaaga attaccgctt tatgaaatag
     9661 gttggcgctt gttgccagaa ttttggggaa aaggattagc aactgaaggc gcaaaggcaa
     9721 cattgaagtt agcagaagaa catcaaatat acgatgtcta tagttttaca gcagaagcaa
     9781 ataaagcttc acaacgtgta atggaaaaaa ttggcatgac agtgtatgat catttcgaat
     9841 tacccaatct aagtaagtat catttattaa aaaggcaagt gcgctattac attaatcttc
     9901 gaaagtgaaa aatttataca taagcgtaac aaacacccct aacattgttt agctgatgat
     9961 aacaatatta ggggtgtttg tttatttttt aaccttagaa tgattaatcg tatgaacgag
    10021 tacccagagg tttgaaattt aatattgatt caattaatga ttccttagtg tcgcataacg
    10081 gtgcaagagc acgatactta ggatcaataa aaccttcctc aatcatatgg tcaatcattg
    10141 tttgtagtgg attgaaaaag ccattaatat tataaatggc aataggcttt tcatggatac
    10201 ctatttgagc ccaactatac atttcgaaaa attcttctag tgaacctgcg ccaccaggag
    10261 ccatgacaaa tgcatctgca agttctgcca ttttattttt acgttcatgc atagaatcaa
    10321 ctaaaattaa ttcagttaaa cgttggcttg tgatttcatg ttcatctaac attttaggca
    10381 tgacgccaat agctttgccg ccatgatcta atacaccatc ttgaatggca cccataatgc
    10441 caattgaccc tgcaccaaat actaattcat aaccttgttc agcaaaatat ttacctaaat
    10501 cgtatgcttt ttgtacatat gaagggtcat gacctttgct tgcaccacaa taaactgcga
    10561 ttcgtttcat gttaatccag ctccttaatt cgatgaatga cttttaatag tgattgttca
    10621 aacacttttt gatcttgctt tgtaaaaggt gggggacctt tgtggcgacc accttgtttt
    10681 ctaatttgtg cattcatata tcgtttatct aatagttgtt gaatattttt ggaattgtat
    10741 atcttcccat tatgatgcat gacaattaag actttgtcga ctaataaact tgcgagtcca
    10801 taatcttgag tgactacgat atcatccttc gttgataatt gaacaatttt gtaatcaact
    10861 gcatctggtc catcatcaac atataatgtt gatacatgtg gaggatataa ttggttcgaa
    10921 aaatggctga agctccgaat aattgtcaca aaaatgcctg tctcagttgt taaatctata
    10981 atagaatcaa caacaggaca agcatctcca tcaataataa tatgtgtcac aattatgcct
    11041 ctgtattgtt ttctttattt tgttgagagg cgcttttggc aacataatct ttatattttt
    11101 taaatgactt gatgcgtgct ttatcagctt cttgttggcg tttttgttct tctttgtgtc
    11161 gtttttcaat atttttttgt aactttttat tcattttagc gatttctttg cgattttttt
    11221 cagctagttt atcgcctttt ttctcagttt tctcatctaa tttattaggt gttaagcctg
    11281 ctttttcttc gtatttttgt gattttttca tatctttaat acgttgtatt tcattctttt
    11341 cgcgggcttt ttgctcttct ttatgacgct tttcgatatt tttttgaagt attttattca
    11401 ttttatcagc gtctttacga ttttgtttag ctaatttttc gccttttttc tcaatatagg
    11461 caggatcatg ttctctagca aactttttaa gttcacgttt attttcaaaa tcttgttttt
    11521 tatcgccgac atattcttta acatcactcg ctgtgttact gattgctgca gatgtttttg
    11581 aagcaacttt acttgtagca tctgtaactt tttgtacgtc cggatgttgt ttgatacgtt
    11641 tacgttcaac aattaacggt accaatacaa ttggtaatac attaatcata aatttgatga
    11701 cttttttctt atccatagat cttgcctcca taattacttt attaatttta cataccctat
    11761 gatacatcaa tataaacgat gatagtagtg aatcactatt aagtatttca gatgtttttt
    11821 aaaagaagac aataaaaact gccaatcaag tgattcctta attgacagtc tatattttaa
    11881 aaggaaatta aataccttta ccaatgccaa atccgaagta aagtatagca ataaagatta
    11941 ctaatacaat tctgtaaatg gcaaatggaa ttagtttgat tttgttaatt agatgcaaga
    12001 atgttttgat tgcaattagt ccaacagtaa atgcagctaa aaagcctaaa atataaaaag
    12061 gtatatcagc aatctgaata tcttgataat gttttaataa agataaacca ctagctgcta
    12121 acataattgg aacagccata ataaatgtaa agtccgatgc tgctttatga tttaatttca
    12181 ttaatacccc agttgaaatt gttgagcctg aacggctgaa accaggccac atagctactg
    12241 cttgagaaat accaattaca aatgcttgga aataactgat ttgatctact gtttgtgggt
    12301 ttttaacttt agctgagtat ttatcagcaa taatcatata gatagcacct acgaataagc
    12361 caatcataac agttggcaca ctaaataaat gttcttcgat gaaatcatca aatagtaagc
    12421 ctaaaatacc tgctggcacc atacccacta atacatgtaa taaatttaaa cgtcttggct
    12481 ttgaacgtct ttgttgatcg ttatctcctt caacatgttt gtgtttacca atatgtaaaa
    12541 tctctaagaa gcgttcgcgg aacacccatg ctgctgcaaa gacggatcct aattggatga
    12601 cgattttaaa tgtaaatgct gactgagaac ctaaaaattc agatgatttt aaccacatat
    12661 catcaactag gatcatatgt ccagtagagg aaacaggtgc aaattctgtt aatccttcga
    12721 cgacccctaa gataatacct tttattaatt caatgataaa cataatgtac ccactttcat
    12781 tactcaattt aatttattta aatatcaaaa ttaccatatc atgatagcat attcatttaa
    12841 agacatgcta gttataatac tagactaaag atgtatatat tcattttctt ttacatgtaa
    12901 aactacaata ttttattgag ctatttaatt tgattttaag gaaaaccttt tataataggt
    12961 ttaggtgata taattgtgaa aaaattaaca acaatactgt ttcaatataa aatttttccg
    13021 gtactcatgt tcttggtcag tactggtctc ggcataatcg ttataacgca aaatatttta
    13081 atagcagatt ttttagctaa aattataaga catcaatttc aaggtttatg gattgtatta
    13141 tttattttat taggtgtttt acttttaaga gcaactgtgc aatttctaaa tcaatggtta
    13201 ggtgatacat tagcatttaa agttaagcat atgcttagac agcgggttat ttataaaaat
    13261 aatggtcatc caatcggtga acaaatgact atactcacag aaaacattga tggtctagca
    13321 cctttttata agagttattt gcctcaagtg ttcaaatcaa tgatggttcc gctcatcata
    13381 atcattgcaa tgtttttcat ccatttcaat accgcattaa ttatgttaat aactgcacca
    13441 tttattcctt tgttttatat tattttcggt ttgaaaacgc gagatgagtc aaaagatcaa
    13501 atgacttatt tgaatcaatt tagtcaacgg tttttaaata ttgctaaagg tttagtgacg
    13561 ttaaagctat ttaatcgtac agagcaaaca gagaagcata tttacgacga tagtactcag
    13621 tttagaactt taacaatgcg cattttacgc agtgcttttt tatcgggatt aatgctcgaa
    13681 tttataagta tgttaggtat tggattggtt gcattggaag caacgctaag cttagtagta
    13741 tttcataata ttgattttaa aactgcggca attgcgatta ttttagcgcc tgaattttat
    13801 aatgcaatta aggacttagg gcaagcgttc catactggaa aacaaagtga aggtgccagt
    13861 gacgttgtgt ttgagttttt agaacaaccg aactataata atgaatttct attaaagtat
    13921 gaggaaaacc aaaagccatt tattcagtta acagacatat catttcgata tgatgattct
    13981 gatagattgg tattaaatga tttaaatttg gaaatattta aaggtgatca aattgcactt
    14041 gtaggtccaa gcggggcagg taaatccact ttgacacatc ttattgcagg tgtttatcag
    14101 ccaacaatag gtactataag tacaaaccag cgtgatttaa atataggaat acttagtcaa
    14161 cagccatata ttttcagtgc ttctataaaa gagaatatta cgatgtttaa agatatagaa
    14221 aataatacta ttgaagaagt gctagacgaa gtaggtttat tagacaaagt gcaatctttc
    14281 acaaaaggca ttaacacaat aataggtgaa ggaggcgaaa tgttatctgg tggacagatg
    14341 agacgcatag aactttgccg tcttttagtt atgaagccag atctcgttat atttgatgag
    14401 cctgcaactg gtttagatat tcaaacagaa cacatgattc agaacgttct gtttcaacat
    14461 tttaaagata caacgatgat tgtcattgca catagagata atacaattcg ccatttacaa
    14521 cgacgcttgt atatagaaaa tggaagactg attgctgatg atcgcaatat ttcagtaaat
    14581 ataacagaaa atggtgatga cttatgaaaa cacgactaaa atttcaagta gataaggatt
    14641 tattgttagc tatagttgtt ggtgtttgtg gaagtttagt tgcgctcgcc atgtttttct
    14701 taagtggtta tatggtgaca caaagtgcac ttggtgcgcc actatacgct ctgatgattt
    14761 tagtcgttac agtaaaattg tttgggtttt taagagctat tactcgatac gtagagcgcc
    14821 ttatttctca taaagctaca tttacaatgc tacgtgatat tcgggtacag tttttcggta
    14881 aattagtaaa tgtcattcct aatgtttacc gtaaactgag ttctagtgat ttaatttcac
    14941 gtatgattag tcgtgttgag gcattacaaa atatatattt acgtgtttat tatccaccag
    15001 tcgtcatcgg tttgacagcg ctagttacag tcatagtttt ggcgttcatt tcaatcggcc
    15061 atgcgctatt gattatggtt agcatgttgt tcactttact cattgttcct tggttaagct
    15121 caaaaaaagc acgtacttta aagaaacatg cagctaatga acaggcccga tttttaaatc
    15181 atttttatga ttataaagct ggtatggatg aactacgtcg atttaatcaa attaatcatt
    15241 atcgagataa tttgatggct aaattaaatc attttgataa attacaactt aaagagcaac
    15301 gctttttaac gatttatgat tttatattaa atattattgc tatgctttcg atttttggta
    15361 gtttagttct aggattaatt caaattaatg caggccaact aaatattatt tatatgacga
    15421 gtatagtttt aatggtctta actttatttg aacaagctgt accaatgaca aatgtcgcgt
    15481 attataaagc ggatactgac caagcattgc acgatattaa tgaagtgata tctgtacctt
    15541 ctactaatgg aaaaaaacgt cttaatgata agtatgatgc aacgaacatt tatgaagtta
    15601 aggatgctag ttttaagtat tggaatcagc aaacgtatgt gttgtcggat attaatttta
    15661 atgttaatag aggcgaaaag attgcgattg tgggtccttc tggttcagga aaaagtacat
    15721 tactacaaat tatggcaggg ttatatcaat tagatagtgg ctctgttcgt ttcgaaaata
    15781 tggatatgtt tgaaatagat gacaaagata agtttgaatc gttaaatgtc ttgctacaat
    15841 ctcaacaatt atttgatggt acaatacgtc aaaatttatt taccgatgaa aaagatgaag
    15901 cggtgcaagc aatatttaag caattagatt tagaacattt ggcactagaa cgtcaaattg
    15961 acttagatgg tcatacatta tctggcggag aaattcagcg tttagcgatt acgaggatgt
    16021 tattaaaaga tactgcatca acatggattt tagatgaacc aacaactgca ttagataaac
    16081 aaaatagttt aaaagttatg gatttaattg aagcacatgc agaaacatta attgttgcta
    16141 cacacgattt aactttattg tcacgttttg agaccatcat tgtgatgata aatggtaaaa
    16201 tagttgaaaa gggaaactat caacaattac tagctaatca aggtgcttta tggaatatga
    16261 ttcaatataa tgcataaaaa aactgcttga taggctaaga aacctttcaa gcagtttttt
    16321 aactaacaag cttacgcgtg ctaattgttt ttctattctt aataaattct aaacattact
    16381 ttaattgtca tgacaaaagt taattatttt tcctttgttt catcaaatgc atgaatgact
    16441 ttacctaata agcgattaag ttctttaact tcatcttgcg ataaagaaga agctgaagcg
    16501 actttgtcag atgcattact taattctggt ctaatagttt cacttttgtc agtcaagtga
    16561 ataaatactt cacgttgatc gacttcggaa cgttcacgct taattaagtc tacttgttcc
    16621 attcgtttta ataatggtga tactgtacca gtatcgagtg ctaattcagt tacgactttc
    16681 ttgacgttta caggagattc atcccataaa attgttaaga caagaaattg tgggtatgtt
    16741 agattgtact tcttaaaaac tttgttagag tagtagcgat taacttgtct ttgagcattg
    16801 tacaaactaa agcatagctg ttcttttaaa ttatgttgat cagacattaa agttctcctc
    16861 cagacatact atccgttttt ttctcttttc ggattggtaa tcattaaaaa gttgattgtt
    16921 tattaattca caactttctt tgattcaatg ccatgctaaa attaaagtat gtttaaagtt
    16981 tagaagatat ttttgattaa atcaagcaa
//
LOCUS       DALAGD010000068        16943 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.069, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000068 DALAGD010000000
VERSION     DALAGD010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16943
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.069"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(114..1241)
                     /locus_tag="O2701_001843"
     CDS             complement(114..1241)
                     /locus_tag="O2701_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCZ4293729.1"
                     /translation="MKSKYEPLFDKVELPNGVELRNRFVLAPLTHISSNDDGTISDVE
                     LPYIEKRSQDVGITINAASNVSDVGKAFPGQPSIAHDSNIEGLKRLATAMKKNGAKAL
                     VQIHHGGAQALPELTPDGDVVAPSPISLKSFGQKQEHSAREMTNEEIEQAIKDFGEAT
                     RRAIEAGFDGVEIHGANHYLIHQFVSPYYNRRNDVWANQYKFPVAVIEEVLKAKEAYG
                     NKDFIVGYRLSPEEAESPGITMEITEELVNKISHMPIDYIHVSMMDTHATTREGKYAG
                     QERLPLIHKWINGRMPLIGIGSIFTADEALDAVENVGVDLVAIGRELLLDYQFVEKIK
                     DGREDEIINYFDPEREDNHHLTPNLWHQFNEGFYPLPRKDK"
     gene            complement(1603..1980)
                     /locus_tag="O2701_001844"
     CDS             complement(1603..1980)
                     /locus_tag="O2701_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HCZ4293730.1"
                     /translation="MNNYKIGQHIKVRVTGIQPYGAFVETPNHTEGLIHISEIMDDYV
                     HNLKKFLSEGQIVKAKILSIDDEGKLNLSLKDNDYFKNYERKKEKQSVLDEIRETEKY
                     GFQTLKERLPIWIKQSKRAIRND"
     gene            complement(2396..2989)
                     /locus_tag="O2701_001845"
     CDS             complement(2396..2989)
                     /locus_tag="O2701_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCZ4293731.1"
                     /translation="MANYPQLNKEVQQGEIKVVMHTNKGDMTFKLFPNIAPKTVENFV
                     THAKNGYYDGITFHRVINDFMIQGGDPTATGMGGESIYGGAFEDEFSLNAFNLYGALS
                     MANSGPNTNGSQFFIVQMKEVPQNMLSQLADGGWPQPIVDAYGEKGGTPWLDQKHTVF
                     GQIIDGETTLEDIANTKVGPQDKPLHDVVIESIDVEE"
     gene            3053..3436
                     /locus_tag="O2701_001846"
     CDS             3053..3436
                     /locus_tag="O2701_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase-associated lipoprotein B"
                     /protein_id="HCZ4293732.1"
                     /translation="MKLYRFSHKTGSYGVTIVEENGDDVLVKVEQVIKHPKQGDLHNP
                     TETEGVFFHDRKALSHFEKRYTKRSQLRDFNVDVLRYEDSLQQAITDLETKLKAQQTK
                     HAEMSLASLARLKEDYSLQYKENFK"
     gene            3567..5972
                     /gene="mnhA1"
                     /locus_tag="O2701_001847"
     CDS             3567..5972
                     /gene="mnhA1"
                     /locus_tag="O2701_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit A"
                     /protein_id="HCZ4293733.1"
                     /translation="MSLLHIAVILPLIFALIIPILYRFFKRIHLGWFVLPVPIVIFIY
                     MLTLIKTTMSGNTVMKTLNWMPHFGMNFDLYLDGLGLLFSLLISGIGSLVVLYSIGYL
                     SKSEQLGNFYCYLLLFMGAMLGVVLSDNVIILYLFWELTSFSSFLLISFWRERQASIY
                     GAQKSLIITVFGGLSLLGGIILLAIPTQSFSIQYMIQHASEIQNSPFFIFAMILIMIG
                     AFTKSAQFPFYIWLPDAMEAPTPVSAYLHSATMVKAGLYLIARMTPIFAASQGWVWTV
                     TLVGLITLFWASLNATKQQDLKGILAFSTVSQLGMIMAMLGIGAISYHYQGDDSKIYA
                     AAFTAAIFHLINHATFKGALFMITGAVDHSTGTRDVKKLGGLLTIMPISFTITVITAL
                     SMAGVPPFNGFLSKESFLETTFTASQANLFSVDTLGYLFPIIGIVGSVFTFVYSIKFI
                     MHIFFGQYKPEQLPKKAHEVSILMLLSPAILATLVIVFGLFPGILTNSIIEPATSSIN
                     HTVIDDVEFHMFHGLTPAFLSTLVIYILGILLIVTFSYWVKLLQRQPGKLTFNYWYNR
                     SANVIPNYSEKMTNSYVTDYSRNNLVIIFGALILLTFVTIFSVPFNINFKDVSPIRIF
                     EVCIVILLLSAAFLILFAKSRLFSIIMLSAVGYAVSVLFIFFKAPDLALTQFVVESIS
                     TALFLLCFYHLPNLNRYNEKRSFQLTNALIAGGVGLSVIIIGLIAYGNRHFESISKFY
                     QEHVYDLAHGKNMVNVILVDFRGMDTLFESSVLGIAGLAVYTMIKLRKKRQTQGNEVK
                     NHE"
     gene            5965..6393
                     /gene="mnhB1"
                     /locus_tag="O2701_001848"
     CDS             5965..6393
                     /gene="mnhB1"
                     /locus_tag="O2701_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit B"
                     /protein_id="HCZ4293734.1"
                     /translation="MNRQQNDLILQFAAVIIFFMVMVFGFSLFLAGHYTPGGGFVGGL
                     LFASSLVIITIAFDIETMRKIFPLDFKILIGIGLVFCIATPIASWFLGKNFFTHVTFD
                     IPLFILEPVHMTTAVFFDFGVLCAVVGTVMTIIISIGENE"
     gene            6393..6734
                     /gene="mnhC1"
                     /locus_tag="O2701_001849"
     CDS             6393..6734
                     /gene="mnhC1"
                     /locus_tag="O2701_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit C"
                     /protein_id="HCZ4293735.1"
                     /translation="MEIIMIFVSGILTAISVYLVLSKSLIRIVMGTTLLTHAANLFLI
                     TMGGLKHGTVPIYEANVKSYVDPIPQALILTAIVIAFATTAFFLVLAFRTYKELGTDN
                     VESMKGVPEDD"
     gene            6727..8223
                     /gene="mnhD1"
                     /locus_tag="O2701_001850"
     CDS             6727..8223
                     /gene="mnhD1"
                     /locus_tag="O2701_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit D"
                     /protein_id="HCZ4293736.1"
                     /translation="MIESNMLVLTLVIPVITAILLVFIGKRPIIKRYVALGGTLLTLV
                     AAIINLANVVKHGPIRVELGSWKAPYSIVFVLDIFSALLIITSIIITAIVILYSYQTI
                     GIERERYYYYFSVLFMLIGIIGAFTTGDIFNLFVYFEVFLMSSYFLLVIGSTKIQLQE
                     TIKYVLVNVVSSSFFVMGVAILYSVVGTLNLADISNKLANLSAHDSGLVNIVFILFIF
                     VFATKAGVFPMFVWLPSAYYAPPIPIIAFFGALLTKVGVYAIARTLSLFFSDNVSFSH
                     YVILFLALLTIIFGCVGAVAYANIKKIILYNVMIAVGVILVGVAMMTESGMIGAIYYT
                     LHDMLVKLALFLLIGIMIKITGTADLRQFGGLIKRYPVLGWSFFIAALSLAGIPPLSG
                     FYGKFFIVQSTFERGFYLSGVIVLLSSLVVLYSVIRIFLQGFFGQPKGYDLNNKVDVK
                     YLTTIAIVAVVITVLYGLSADYLYPMVKAGAETFYNPSTYVKAVLGGK"
     gene            8225..8704
                     /gene="mnhE1"
                     /locus_tag="O2701_001851"
     CDS             8225..8704
                     /gene="mnhE1"
                     /locus_tag="O2701_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit E"
                     /protein_id="HCZ4293737.1"
                     /translation="MAVQLVLNFIIAVFWLFVTNSYTTNNFVLGFIFGLVLVYLLHRV
                     LPGRFYVITLYRIIKLVIIFLIELIKANFDVLKIIIKPSIKNEPGFFVYHTDLKKDWQ
                     IVLLSNLITLTPGTVVLGVSDDRTKIYIHAIDFSTKEQEVESIKTSLEKIVREVGEI"
     gene            8704..8997
                     /gene="mnhF1"
                     /locus_tag="O2701_001852"
     CDS             8704..8997
                     /gene="mnhF1"
                     /locus_tag="O2701_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit F"
                     /protein_id="HCZ4293738.1"
                     /translation="MNHNVIIVIALIIVVISMLAMLIRVVLGPSLADRVVALDAIGLQ
                     LMAVIALFSILLNIKYMIVVIMMIGILAFLGTAVFSKFMDKGKVIEHDQNHTD"
     gene            8975..9331
                     /gene="mnhG1"
                     /locus_tag="O2701_001853"
     CDS             8975..9331
                     /gene="mnhG1"
                     /locus_tag="O2701_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit G"
                     /protein_id="HCZ4293739.1"
                     /translation="MIKIILISLALIFVIIGALISALAAIGLLRLEDVYSRAHAAGKA
                     STLGAMSLLFGTFLYFIATQGFVNMQLIVAIIFVLITGPLSSHMIMKAAYNIKTPYTK
                     KTKVDEISEDLKDTKL"
     gene            9580..10734
                     /locus_tag="O2701_001854"
     CDS             9580..10734
                     /locus_tag="O2701_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCZ4293740.1"
                     /translation="MHWTIIGGGIQGTAIAQKLLSSGLTTDRLTIIDPHETFCQRFNS
                     YTNRIEMPYLRSPIVHHVHPQPFHLKQFAKQHQYTNAFYGPYQRPELTMFMDHIAHAS
                     KQYQLEDCLVQGLVQTLDKQEDKWHIKLEDGQIITTDCVVIAIGSTNIPFMPDILKDK
                     QNVNHIFEKELDQVVYDKTDHIVGSGITAAHLALKLLNHDNDKKIHLWLNKDIEIHDF
                     DADPGWLGPKNMSSFLSTKSMPERNAIVQRERHKGSMPHELYLRLKKHIKNGRINVHK
                     TPITQISGGVINTENDSVPYQQIMVATGFEQDFMSQPLIKQLIQNYDAPINECNYPVI
                     SEKLEWIPNLFVAGCFADLELGPFGRNVMGGRKAAERIEQAFLKLQQYSA"
     gene            complement(10790..11164)
                     /locus_tag="O2701_001855"
     CDS             complement(10790..11164)
                     /locus_tag="O2701_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HCZ4293741.1"
                     /translation="MTHLLETFEMSIDHQEDGLVVISMPVTDKVKQPFGYLHGGASIA
                     LGETACSLGSANLIDTTKFIPLGLEMNANHIHSAKDGRVTATAEIIHRGKSTHVWDIK
                     IKNDKEQLITVMRGTVAIKPLK"
     gene            complement(11183..12499)
                     /locus_tag="O2701_001856"
     CDS             complement(11183..12499)
                     /locus_tag="O2701_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HCZ4293742.1"
                     /translation="MINAVVIAVILMIVLCLCRLNVVISLFISALVGGLISGMSIEKV
                     INVFGKNIVDGAEVALSYALLGGFAALISYSGITDYLVGKIINAIHAENSRWSRVKVK
                     VTIIIALLAMSIMSQNLIPVHIAFIPIVIPPLLSLFNDLKIDRRLIGLIIGFGLCFPY
                     VLLPYGFGQIFQQIIQSGFAKANHPIEFNMIWKAMLIPSMGYIVGLLIGLYVYRKPRE
                     YETRKISDSDNVTELKPYILIVTIVAILATFLVQTFTDSMIFGALAGVLVFFISRAYN
                     WYELDAKFVEGIKIMAYIGVVILTANGFAGVMNATGDIDELVKTLTSITGDNKLFSII
                     MMYVIGLIVTLGIGSSFATIPIIASLFIPFGASIGLDTMALIALIGTASALGDSGSPA
                     SDSTLGPTAGLNVDGQHDHIRDTCVPNFLFYNIPLMIFGTIAAMVL"
     gene            complement(12910..14385)
                     /locus_tag="O2701_001857"
     CDS             complement(12910..14385)
                     /locus_tag="O2701_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HCZ4293743.1"
                     /translation="MNFKLNNTLSNEINTLIIGIPEHLNQLERISFNHIDITESLERL
                     KHQHIIGSKVGKIYTTAFDVQDQTYRLITVGLGNLKTRSYQDMLKIWGHLFQYIKSEH
                     IEDTYLLMDSFISKYDQLSDVLMACGIQSERATYEFDHYKSSKKAPFKTNLNLISESL
                     IELDFIHEGISIGQSINLARDFSNMPPNVLTPQTFAEDIVNHFKNTKVKVDVKDYDTL
                     VSEGFGLLQAVGKGSKHKPRLVTITYNGKDKDEAPIALVGKGITYDSGGYSIKTKNGM
                     ATMKFDMCGAANVVGIIEAASRLQLPVNIVGVLACAENMINEASMKPDDVFTALSGET
                     VEVMNTDAEGRLVLADAVFYANQYQPSVIMDFATLTGAAIVALGDDKAAAFESNSKVI
                     LNDILQISSEVDEMVFELPITATERASIKHSDIADLVNHTNGQGKALFAASFVTHFSG
                     QTPHIHFDIAGPATTNKASYNGPKGPTGFMIPTIVQWLKQQ"
     gene            complement(14516..15724)
                     /locus_tag="O2701_001858"
     CDS             complement(14516..15724)
                     /locus_tag="O2701_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCZ4293744.1"
                     /translation="MAQDRKKVLVLGAGYAGLQTVTKLQKAISTEEAEITLINKNEYH
                     YEATWLHEASAGTLNYEDVLYPVESVLKKDKVNFVQAEVTKIDRDAKKVETNQGIYDF
                     DILVVALGFVSETFGIEGMKDHAFQIENVITARELSRHIEDKFANYAASKEKDDNDLS
                     ILVGGAGFTGVEFLGELTDRIPELCSKYGVDQNKVKITCVEAAPKMLPMFSEELVNHA
                     VSYLEDRGVEFKIATPIVACNEKGFVVEVDGEKQQLNAGTSVWAAGVRGSKLMEESFE
                     GVKRGRIVTKQDLTINGYDNIFVIGDCSAFIPAGEERPLPTTAQIAMQQGESVAKNIK
                     RILNGESTEEFEYVDRGTVCSLGSHDGVGMVFGKPIAGKKAAFMKKVIDTRAVFKIGG
                     IGLAFKKGKF"
     gene            complement(16178..16537)
                     /locus_tag="O2701_001859"
     CDS             complement(16178..16537)
                     /locus_tag="O2701_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HCZ4293745.1"
                     /translation="MPTVILTEAAAYEVKDMLKANEMPDGYLKIKVNGGGCTGLTYGM
                     GAEEAPGENDEVLEYFGLKVLVDKKDAPVLNGTTIDFKQSLMGGGFQIDNPNAIASCG
                     CGSSFRTAKVAGNPENC"
     gene            complement(16550..16786)
                     /locus_tag="O2701_001860"
     CDS             complement(16550..16786)
                     /locus_tag="O2701_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzB family protein"
                     /protein_id="HCZ4293746.1"
                     /translation="MNPIVEFCLSNMAKGGDYVFNQLENDPDVDVLEYGCLTHCGICS
                     AGLYALVNGDIVEGDSPEELLQNIYAHIKETWIF"
BASE COUNT         5362 a         3012 c         2552 g         6017 t
ORIGIN      
        1 ataattttac acttaatcaa aagtatattt tactaagaat atttttattt ataaatattg
       61 aaaaccacta acaaattgca tacacaatat cattagtggt aacagttaaa cacttattta
      121 tctttacgtg gtaatggata aaacccttca ttaaattgat gccataaatt aggagttaag
      181 tgatgattat cttctctctc tggatcaaag taattaataa tttcatcttc ccgtccatct
      241 ttaatttttt caacaaattg ataatccagt agtagctctc taccaatggc tactaagtca
      301 acaccaacat tttcaactgc atctaaagct tcgtcagctg tgaaaattga accaataccg
      361 ataagtggca tacgaccatt tatccatttg tgaattaaag gcagtctttc ttgtccagcg
      421 tatttacctt cacgtgtcgt tgcatgcgta tccatcattg aaacatgaat atagtcgatt
      481 ggcatatggc taattttatt aacgagttcc tctgtaattt ccattgtgat tcctggagac
      541 tccgcttcct ctggagataa tctgtatcca actataaagt ctttattgcc atacgcttct
      601 ttcgctttaa gtacttcttc aatcacagcg accgggaatt tatattgatt tgcccataca
      661 tcatttcttc tattatagta tggtgataca aattgatgaa ttaagtaatg attcgcgcca
      721 tgtatttcaa caccatcaaa ccctgcttca attgcacgtc gcgttgcttc accaaaatcc
      781 ttgattgctt gttcaatctc ttcattcgtc atttctctag cactatgttc ttgtttctga
      841 ccaaaacttt ttaaagaaat tggacttggt gctacgacgt ctccatcagg tgttaattca
      901 ggcaatgctt gtgcaccgcc atgatgtatt tgtacgagtg ctttggcacc gtttttcttc
      961 attgctgtag ctaatcgttt tagtccttca atattactgt catgcgcgat tgatggctgt
     1021 cctggaaatg cttttccgac atcactcaca ttactcgcag cattaattgt aataccaaca
     1081 tcttgtgaac gcttttcaat ataaggaagt tctacatctg aaatagtacc atcatcattt
     1141 gaagaaatat gtgttaaagg ggctaacaca aatcgatttc tcaactctac tccatttggt
     1201 aattctactt tatcaaacaa tggttcgtat ttacttttca ttacttaatt cctccttaaa
     1261 attattgagt tgactataac atgtaatttt ttcattctcc aattatctgt ttatatagtt
     1321 gcaattcgtt taatacttta agttgtagtt ctcatttcta aaataaataa aaaaaacaaa
     1381 tccacataca atattgcaaa tcaacgattc tatctaacaa agataatgga tatgtcacct
     1441 taaaccattt gtacgtgtaa atagaatcaa tcatattacg ttatcgtaga caaaaagaga
     1501 ttagtatcta ataaaattta atagatacta atctctttta tagtctaaga aactttcatt
     1561 tcagaccctt tgtatgattt tcggtacgat ttatctgttc ctttagtcgt ttcgaattgc
     1621 tcgctttgac tgttttatcc agattggtaa gcgttcttta agtgtttgaa acccatattt
     1681 ttctgtttct ctgatttcat ctaatactga ttgtttttcc ttcttacgct cataattttt
     1741 gaagtaatca ttatccttta atgatagatt aagctttcct tcatcatcta tagacaaaat
     1801 tttagcttta acaatttggc cttctgatag aaatttcttc aaattatgaa cgtagtcatc
     1861 cataatttct gatatatgaa tcagtccttc agtatgatta ggggtctcaa caaacgcacc
     1921 gtatggttga ataccagtta cacgcacctt gatatgttgg ccaattttgt agttattcaa
     1981 cttaataacc ccttatttcg tttacttaac actattatca tagcacaaat cgaaatggtg
     2041 gactaattaa aacatcaaat catacgaata ttattttata aatgaataag aattctacaa
     2101 acttggattt acaaataaaa ttagcaacaa attcctatct aaatcaatat gatatccagc
     2161 tactttgttt tcaatttatt tactatttga atagcaattc tctgtaaaaa cataaaaagc
     2221 aacctcaact attatcttta aaatacacat ctatttaaaa actgtgcttt cttgataaat
     2281 atattgagat tgctcatcat attagtttat ttattttcgt tggaatatat actaatccat
     2341 tcataaatta gctttatccg agtttaaaat gttggtagtt aattatgttt agatattatt
     2401 cttcaacatc aatagattca attacaacat catgaagtgg tttatcttgt ggtcccactt
     2461 ttgtatttgc aatatcttct aatgtagttt caccatcaat gatttgaccg aatactgtat
     2521 gtttttgatc taaccatggt gtaccaccct tttcgccata tgcatcaacg attggttgag
     2581 gccagccacc atctgcaagt tgacttaaca tattttgagg tacttctttc atttgaacaa
     2641 tgaaaaattg tgaaccatta gtattaggtc ctgagttagc cattgataat gcgccatata
     2701 agttaaatgc atttaatgaa aattcatctt caaaagcacc gccataaata ctttcgccac
     2761 ccataccagt agctgttgga tcgccacctt gaatcatgaa gtcattaatg acacggtgga
     2821 atgtgattcc atcataataa ccattttttg catgtgtcac aaaattttca actgtttttg
     2881 gtgcaatatt tggaaataat ttgaatgtca tgtcaccttt atttgtgtgc ataaccactt
     2941 tgatttcacc ttgttgtact tctttgttta actgtggata gttagccatt tatattctcc
     3001 attcatgtta tgatacatat atattaacat acttttaagg gaggaaaata gcatgaagct
     3061 atatcgattt tcacacaaaa caggtagcta tggtgtcacg attgttgaag aaaatggaga
     3121 cgatgtcctt gttaaagttg aacaagttat caaacacccg aaacaaggtg atttacataa
     3181 ccctactgaa actgaaggtg tatttttcca tgaccgcaaa gcattaagcc attttgaaaa
     3241 acgctatacg aaacgttcac aattacgcga cttcaatgtt gatgtattgc gctatgaaga
     3301 ttcacttcaa caagcaatta cagatttaga aactaaactt aaagctcagc aaacaaaaca
     3361 tgctgaaatg tctttagcta gtttagcacg cttaaaagaa gattattcac ttcaatacaa
     3421 agaaaatttt aaataaaaaa tgacgtgttt cttcttattt aaatacttct gaaaataaag
     3481 cacaacaagg agtgtgtacg tttttcagaa ctatatcatt catgtataat gaataacata
     3541 aatatttttt aaaggaggac aacatagtga gtttgttaca tattgcggtg atattaccgt
     3601 taatattcgc actcattatt cctatcctgt atagattttt taaacgtata catttaggat
     3661 ggttcgtatt acctgtccct atcgtcatat ttatttatat gcttacatta attaaaacga
     3721 cgatgtcagg taatactgta atgaaaacct taaattggat gcctcatttt ggtatgaact
     3781 ttgatttata tcttgatggc ttaggtttac tctttagttt gcttatttca ggaatcggca
     3841 gtttagtagt attatattca atcggctact taagtaagtc agaacaacta gggaattttt
     3901 attgttattt attactattt atgggtgcaa tgttaggtgt cgtactttca gataatgtta
     3961 ttattctata tttattctgg gagctaacgt cattttctag ttttttactt atttcattct
     4021 ggcgcgaacg acaagcatct atatatggtg ctcaaaaatc gctcataatc acagtatttg
     4081 gtggtctatc attattaggt ggtatcatct tactcgctat accaacacaa tcatttagca
     4141 ttcagtacat gattcaacat gcttctgaaa ttcaaaatag cccattcttc atttttgcga
     4201 tgattttaat catgattggt gcttttacaa aatctgctca gtttccattt tatatttggc
     4261 taccagatgc aatggaagca cctacaccag tcagtgctta tcttcattca gcaacaatgg
     4321 ttaaagcagg gttatattta atcgctcgaa tgacaccaat cttcgcagca tcgcaaggtt
     4381 gggtatggac tgtcacactc gttggtttaa ttacattatt ctgggcttcg ctaaacgcaa
     4441 caaaacaaca agacttgaaa ggtatactgg cattttcaac agtatcccaa ttaggtatga
     4501 ttatggcgat gcttggaatc ggtgccatta gttatcacta tcaaggcgat gatagcaaaa
     4561 tatatgctgc tgcatttacg gctgcaatat tccatttaat caaccatgca acatttaaag
     4621 gtgccctatt tatgattaca ggtgcagtgg atcattctac tggtacacgt gatgttaaga
     4681 aattaggtgg cttactaaca attatgccta tttcatttac aatcactgtt attacagcat
     4741 taagtatggc tggtgtacca ccgttcaatg gtttcttatc aaaagaatca tttttagaaa
     4801 caacatttac agcaagccaa gcaaatttat tcagtgttga tacattaggc tacctattcc
     4861 caatcattgg tatagtgggc agtgtcttca catttgtata ttcaattaaa tttataatgc
     4921 atatattttt cggacaatat aaacctgaac aattacctaa aaaagcacac gaagtatcaa
     4981 tattaatgct tttatcacca gctattttag ctacattagt aattgtattt ggtttattcc
     5041 ctggcatttt aactaattcg attattgaac cagctacatc ttcgattaat catacggtaa
     5101 ttgatgatgt tgaattccat atgttccatg gattaacgcc tgccttttta tcaacattag
     5161 tgatttatat tttaggtatc ttactaattg tgacatttag ttattgggtg aaattgttgc
     5221 aacgacaacc tggtaaacta acattcaatt attggtacaa cagaagtgca aatgttattc
     5281 caaactactc tgaaaagatg acgaatagct atgttaccga ttattcacgt aataatttag
     5341 ttatcatttt tggtgcatta attttattaa catttgtcac tatattctca gtacctttca
     5401 acattaattt taaagacgtt agtccgatta gaattttcga agtttgtatc gtaattttat
     5461 tattaagtgc tgccttttta attcttttcg ctaaatcaag attatttagc atcattatgt
     5521 taagtgcagt tggatatgct gtgtctgtat tgtttatatt ctttaaagca cccgacttag
     5581 cattgacaca gtttgtcgtt gaatctattt caacagcatt gttcttatta tgcttctacc
     5641 acttaccaaa tttaaatcgt tataatgaaa agcgctcatt ccagcttact aatgctttaa
     5701 ttgctggtgg tgttggtttg tccgtcatta ttatcggttt aattgcatac ggaaatagac
     5761 attttgaatc gatatctaag ttttatcaag agcacgttta tgatttagct cacggtaaaa
     5821 acatggtaaa cgtcatactt gttgatttcc gtggtatgga tactttattc gagtcatctg
     5881 tgcttggtat cgcaggatta gctgtatata caatgattaa attacgtaaa aagcgtcaaa
     5941 cgcaaggtaa cgaggtgaaa aatcatgaat agacaacaaa atgatctaat tttgcaattt
     6001 gctgcagtta ttatcttttt catggtaatg gtctttggtt tttcactttt cttagcagga
     6061 cattatacac ctggtggcgg tttcgtcggc ggattattat tcgcaagttc acttgttatc
     6121 attacgattg catttgatat agaaacaatg cgtaaaattt tcccattaga ttttaaaatc
     6181 ttgataggta tcggattagt tttttgtatt gcgacgccta tcgcaagttg gtttttaggc
     6241 aaaaatttct ttacgcatgt cacatttgat ataccgttat ttatattaga acctgttcat
     6301 atgacaacag cagtattctt tgatttcggt gttttatgtg cagtagttgg tactgtcatg
     6361 acaattatta tttcgattgg agagaacgaa tagtggaaat tattatgatt tttgttagtg
     6421 gtattctcac agcaattagt gtctatctcg ttttgtctaa aagtctgata cgaattgtta
     6481 tgggaactac actattaaca catgcagcaa atttattttt aataactatg ggcggactta
     6541 aacatggtac tgttccaatt tatgaagcga acgtaaaaag ctatgttgat cctatcccgc
     6601 aagcacttat tttaacagca atcgttatcg cctttgcgac aacagccttt ttcttagtat
     6661 tagcatttag aacatataaa gaattaggca cagataacgt tgagagtatg aaaggagttc
     6721 cagaagatga ttgaaagtaa tatgcttgtg ttaacgctag ttattccagt aattacagcc
     6781 atattacttg tctttattgg aaagcgtcct attataaagc gttatgtcgc actaggcggt
     6841 acattattaa ccttggtcgc ggcaattatc aatttagcaa atgttgttaa acatggacca
     6901 atacgcgtag agctaggttc atggaaagca ccatacagta ttgtctttgt attagacata
     6961 ttcagtgccc tactaataat tactagtatt attattacag ctattgtgat tttatattca
     7021 taccaaacaa ttggtattga acgtgaaaga tattattatt atttttcagt actatttatg
     7081 ttaataggta tcattggtgc attcacaact ggtgacattt tcaacctatt cgtatatttc
     7141 gaagtatttt taatgtcatc ttatttctta ttagtaattg gttctacaaa aattcaacta
     7201 caagaaacaa taaaatatgt tcttgttaac gttgtatcgt catccttctt cgtaatgggt
     7261 gtagcgattt tatattcagt agtaggaaca ttgaatctag cagatataag caataaatta
     7321 gctaaccttt cagcgcatga cagtggctta gtcaatatcg ttttcatact atttatcttt
     7381 gtttttgcta caaaagctgg cgtattccca atgtttgttt ggttacctag tgcatactat
     7441 gcaccaccaa ttccaatcat tgcattcttt ggtgctttgt taacaaaagt tggtgtttat
     7501 gcaatcgcta gaacattgag cctattcttt agtgataatg taagcttttc acattatgtc
     7561 attttattct tagcactatt aacgatcatc tttggttgtg tcggtgctgt tgcatatgcc
     7621 aacattaaaa aaatcatcct atacaatgtc atgattgctg taggtgtgat cttagtcggc
     7681 gttgcaatga tgacagagtc aggtatgatt ggggctattt actatacatt gcacgacatg
     7741 ttagtaaaac ttgcattatt cttgcttatc ggaattatga ttaaaattac aggcactgct
     7801 gatttaagac aatttggtgg cttaattaaa cgatatcctg tacttggttg gtcattcttt
     7861 atcgcagcat taagtttagc tggcattccc ccactcagtg gcttttatgg aaaatttttt
     7921 attgtccaat caacatttga aagaggattt tatttaagtg gtgtcatcgt cttattatca
     7981 agtttagtcg tattatattc tgtcattcgc atattcctac aaggcttttt cggtcaacca
     8041 aaaggatatg atcttaataa caaagtcgac gtcaaatatt taactacgat tgcaatcgtt
     8101 gcagttgtga taactgtttt atatggattg tcagcagatt acttatatcc aatggtaaaa
     8161 gctggtgcag aaacattcta caatccatca acctacgtta aagctgtatt aggaggtaaa
     8221 taacatggct gtacaattag tattaaactt tattatcgca gtgttttggt tgtttgtgac
     8281 aaatagttac acaacaaata actttgtgtt aggtttcatc tttggcctag ttcttgttta
     8341 cctcttacac agagtgttac caggtagatt ttatgtcatc acgctatatc gtattattaa
     8401 attagttatt attttcttaa tcgaactgat taaagctaat tttgatgttt tgaaaattat
     8461 tattaaacct tcgattaaaa atgaaccagg attctttgtt tatcacacag atttaaaaaa
     8521 agattggcaa attgtcttgc tgtctaattt aattacatta acacctggta ctgtagtgct
     8581 tggtgtaagt gacgatcgta caaaaattta tattcatgcc atagattttt caactaaaga
     8641 gcaagaagta gaaagtatta aaacatcatt agagaaaatt gtaagagagg tgggcgaaat
     8701 ataatgaatc ataatgttat tatcgttatt gcattaatca tagttgtcat ttctatgtta
     8761 gctatgctca ttcgcgttgt gctaggccca tcacttgccg atcgtgttgt cgcattagat
     8821 gcgattggtc ttcaattaat ggcagttata gcattattca gtattttatt aaatattaaa
     8881 tacatgattg tcgttattat gatgattggt atattagctt ttttaggtac tgcagtattc
     8941 tctaaattta tggacaaagg taaggtgatt gaacatgatc aaaatcatac tgattagtct
     9001 tgcacttatc tttgttatca tcggtgcttt aattagcgcc ctagcagcta taggattatt
     9061 gagacttgaa gatgtatatt cacgtgcaca tgctgccgga aaagcatcaa cattaggtgc
     9121 aatgtcatta ctatttggta cgtttttata ttttattgct actcaaggtt ttgtaaatat
     9181 gcaattaatc gttgcgatta tctttgtatt aattacaggt cctctttcaa gtcatatgat
     9241 tatgaaagca gcatataata ttaaaacgcc ttatactaaa aagactaaag tcgatgaaat
     9301 ttcggaagac ttaaaagaca caaaattgta gattatataa caaaatcatg aatataaatc
     9361 aacaacaaac agcagtaaga tgattccaaa ttaggaatga ttttactgct gttttctttt
     9421 gacattgtta cctctttttc aatgattttt tctttgacta cagattcgcc ctatctacat
     9481 atatctcttt aatttaattg cctttcatgt cgttatgtat tatgataata ataattataa
     9541 atcgtaacga ttacgtttta aaaagagaga ggttttatta tgcattggac aattatcggc
     9601 ggtggcatac agggaactgc aatcgcacaa aaactattat caagcggatt aacaacagac
     9661 cgattaacaa tcattgaccc acacgaaact ttttgccaaa ggtttaactc atatacaaat
     9721 cgaatagaaa tgccttattt aagatcaccg attgtacatc acgtacatcc acaaccgttc
     9781 catctaaaac aattcgctaa acagcaccaa tatacaaatg ctttttatgg tccttatcaa
     9841 cgacctgaat tgacaatgtt tatggatcat attgcacatg cttctaaaca atatcaatta
     9901 gaggattgct tggttcaagg tttagttcaa actttagata aacaagaaga caaatggcat
     9961 atcaagttag aagatggaca aattatcact acagattgcg tcgttattgc aataggcagt
    10021 acaaatattc cgtttatgcc tgacatttta aaagacaaac agaatgtaaa tcatatcttc
    10081 gagaaagaac ttgatcaagt agtatatgat aagaccgatc atatcgttgg tagcggcatt
    10141 actgctgcac atcttgcact taaattgtta aatcatgata acgataaaaa gattcattta
    10201 tggctaaata aagatattga aatacatgac tttgatgctg atcctggttg gttaggtccg
    10261 aaaaatatgt cttcattttt aagtactaaa agcatgcctg aaagaaatgc cattgtacaa
    10321 cgcgaacgtc ataaaggatc aatgcctcac gaactgtact tacgccttaa aaaacatatt
    10381 aaaaatggtc gtataaatgt gcataaaaca cctatcactc aaattagtgg tggtgtaatt
    10441 aacactgaaa atgattctgt tccatatcaa cagattatgg ttgcaactgg ttttgaacaa
    10501 gattttatgt cacaaccact tattaagcaa ttaatacaaa attatgatgc acctatcaac
    10561 gaatgtaatt accctgttat ttccgaaaaa ttagaatgga taccaaatct atttgtcgca
    10621 ggatgctttg cagacttaga attaggacca tttggtagaa atgttatggg tggccgtaaa
    10681 gctgccgaac gcattgaaca agcatttcta aaactacaac aatatagcgc ataaaaaaac
    10741 tgctagccgt agttatgaat atctcataac atttcagcta gcagttcttt tattttaaag
    10801 gtttaatagc aactgtacca cgcataactg taattaattg ttctttgtca ttcttaattt
    10861 ttatatccca tacatgtgtc gacttacctc gatgaataat ttcagctgtc gcagtaacac
    10921 gaccatcttt agcagaatga atatggttag cattcatctc taaacctaat ggaataaatt
    10981 tggttgtatc aattaaatta gcagatccta atgaacatgc tgtttcacct aaagcaatcg
    11041 aagccccacc atgtaaatat ccaaatggtt gttttacttt atcagtaaca ggcatagaaa
    11101 taacaactaa accatcttcc tggtgatcta ttgacatctc aaatgtctct aataaatgag
    11161 tcatattttc acctctttat gcttatagta ccatagcagc aatagtaccg aaaatcatta
    11221 aaggaatatt ataaaacaag aagtttggta cacatgtatc acgtatatga tcatgttggc
    11281 catcaacatt taatcccgca gttggtccta atgttgaatc acttgcaggc gaacctgagt
    11341 cacctaatgc actcgctgtt ccaatcaatg cgattaatgc cattgtatct agtccaattg
    11401 acgctccaaa aggaatgaat aatgatgcga taataggaat tgttgcaaat gatgatccaa
    11461 tacctaaagt gacaattaaa cctatcacat acatcatgat aatgctaaat aatttattat
    11521 caccagtaat acttgttaaa gttttaacta attcatctat atcaccagta gcattcatta
    11581 caccagcaaa tccatttgct gttaaaataa ctacaccaat ataagccata attttaatac
    11641 cttcaacaaa cttagcatct aattcatacc aattatatgc acgtgaaata aagaatacga
    11701 gtacccctgc cagtgcacca aaaatcattg aatctgtaaa tgtttgtact aaaaatgtag
    11761 ctagtattgc tacaattgtt actattaaga tatatggttt taactctgta acattgtcac
    11821 tatctgaaat tttacgtgtt tcatattcac gtggtttacg atatacatat aaaccgataa
    11881 gtaagccaac aatatacccc attgaaggaa taagcattgc tttccaaatc atattaaact
    11941 caattgggtg atttgccttt gcaaagccac tttgaataat ttgctggaaa atttgaccga
    12001 atccatatgg taataacaca tacgggaaac ataaaccaaa accgataatc aaaccgatta
    12061 aacgtctatc tatttttaag tcattaaaca gacttaacaa tggtgggatg acaattggaa
    12121 tgaatgcaat atgtacagga attaagtttt gactcatgat actcatagct aataatgcaa
    12181 tgattattgt cactttgact ttaactcttg accatcgact attttcagcg tgaattgcat
    12241 ttataatttt tcctactaaa tagtctgtga taccactgta tgaaattaat gctgcaaatc
    12301 cacctaataa agcatagctt aatgctacct cagcaccatc gactatattt ttcccaaata
    12361 catttataac tttttcaatg ctcatgcctg aaattaagcc accaactagc gcactgataa
    12421 ataagcttat aactacgttt aatcgacata aacatagcac aatcattaaa attactgcta
    12481 ttactactgc atttatcata tgacaaccat cctttatcac tctatcgaac taaagcgttt
    12541 atagactaca tagtatcagt agctttaaca cctgtcaata gcaaactata ccaataatct
    12601 ttatttttta gttaattgtc tttcttaaaa taattttagc tttcattaaa ttaaacaatt
    12661 ttacaagctt ggaacaccaa tcaaaatcct aagttctaaa atgcaatatt agtagtcgtt
    12721 gactgaatga acatatgctt ataatatttt tttgcaatgc tagtcaagtt gatttatgct
    12781 cacaaggata tgcgatttat attttcttac aacaatgaaa atgcctgata caatgcgatc
    12841 ctttatactt tcaagagtta aattagaaat gtatatcaag acacaagttg tatcaggcaa
    12901 ataattaaat tattgttgtt ttaaccattg tactatcgtc ggaatcataa atcctgttgg
    12961 cccttttgga ccattatatg aagctttatt agtcgttgct ggacctgcaa tatcgaaatg
    13021 aatgtgaggt gtttgaccac taaaatgtgt tacaaaactt gccgcaaata gcgctttacc
    13081 ttgtccattc gtatggttaa ctaaatcagc gatatcactg tgtttaatac ttgcacgttc
    13141 ggttgcagta atcggtaatt caaataccat ttcatcgact tcagaactta tttgtaatat
    13201 atcgtttaat atcactttac tattcgattc aaatgcagca gctttatcat cgcctagtgc
    13261 aacaattgct gcacccgtta atgtagcaaa gtccataatc acactaggct gatattgatt
    13321 tgcataaaac acagcatctg caaggactaa tctaccttca gcgtctgtat tcattacttc
    13381 tacagtttca ccacttaatg ctgtaaatac atcatctggc ttcattgatg cttcatttat
    13441 catattttca gcacacgcaa gcactccgac aatatttaca ggcagttgta aacgactagc
    13501 cgcttcaatg ataccaacga cattcgcagc gccacacatg tcaaacttca ttgtagccat
    13561 gccattcttc gttttaatac tataaccacc agaatcatac gttatacctt taccaactaa
    13621 ggcaattggt gcttcatctt tgtctttgcc attatatgtg atggttacta atctcggttt
    13681 atgcttacta cctttaccta ctgcttgtaa aagtccgaat ccttcagaaa ctaaagtgtc
    13741 ataatcttta acatctactt tgacctttgt atttttaaaa tgattaacaa tatcttctgc
    13801 aaatgtttgt ggtgttaata cattcggtgg catattacta aagtctcttg ccaaattaat
    13861 ggattggcca atactgatac cctcatgaat aaaatctaat tcaattaatg attcactaat
    13921 aaggtttaaa ttcgtcttaa acggtgcctt cttacttgat ttataatgat cgaattcata
    13981 tgttgcacgc tcactttgaa taccgcatgc cattaataca tctgataact gatcatattt
    14041 tgaaataaat gaatccataa gtaaatacgt atcttcaatg tgttctgact ttatgtattg
    14101 gaaaagatgt ccccatattt tcaacatatc ttgataacta cgtgtcttta agtttcctaa
    14161 accaactgta attaaacgat atgtttgatc ttgtacatca aatgcagttg tataaatctt
    14221 cccaacttta ctaccaataa tatgttgatg ttttagtctt tcaagtgatt ctgtaatatc
    14281 gatatgatta aaactaatgc gctctaactg atttaaatgt tctggtatac caataatcaa
    14341 tgtatttatt tcgttgctta gtgtgttatt taatttaaaa ttcataatgt acctccttag
    14401 atttgatatg ttactcactt caaattgtac aacaataaag cccttcagtg acactgaagg
    14461 actttataaa tgaataattt aattttatgt gtttagctga aactttattt aaagcctaga
    14521 atttaccttt tttgaatgct aaaccgatac caccgatttt gaataccgca cgtgtatcaa
    14581 tcactttttt catgaatgct gcttttttac cagcgatagg tttaccaaat accataccta
    14641 caccgtcatg tgaacctaaa gaacaaacag ttccacgatc aacgtattcg aattcttcag
    14701 ttgattcacc gtttaagatg cgtttaatgt ttttagcaac actttcacct tgttgcattg
    14761 caatttgtgc tgtagttggt aatggacgtt cttctccagc tgggataaac gctgaacagt
    14821 caccaataac aaaaatgttg tcgtaaccat tgattgttaa atcttgcttt gtaacgatac
    14881 gtccacgttt aacgccttca aatgattctt ccattaattt actaccacgt acaccagctg
    14941 cccatactga agtacctgca tttaattgtt gtttttcacc atctacttca actacaaaac
    15001 ctttttcgtt acaagcaacg attggtgtag caattttaaa ttcaacaccg cggtcttcta
    15061 agtagctaac tgcgtggtta actaattctt ctgagaacat tggtaacatt ttaggtgctg
    15121 cttcaacaca agtgatttta actttatttt gatccacacc atatttgcta cataattcag
    15181 gaattctgtc tgttaattca cctaagaatt caacaccagt gaatccagca ccaccaacta
    15241 agatagataa atcgttatca tctttttctt ttgatgctgc atagttagca aatttgtctt
    15301 cgatatgacg tgataattca cgtgctgtga taacattttc aatttggaaa gcatgatctt
    15361 tcataccttc gatgccgaat gtttcactaa cgaaacctaa tgctactact aaaatatcaa
    15421 agtcataaat accttgattt gtttctacct ttttagcatc acggtcaatt tttgttactt
    15481 ctgcttgaac aaagttcact ttgtctttct tcaagacact ttccacagga tataatacat
    15541 cttcatagtt tagtgtacct gctgatgctt catgtaacca tgttgcttca tagtgatatt
    15601 catttttatt aataagcgta atttctgctt cttctgttga tatcgctttt tgcaatttag
    15661 ttacagtttg taaacctgca taaccagcac caagtacaag tactttttta cgatcttgag
    15721 ccatttaatt tcacctaagc tttcatattt ttttaaccaa atgctgataa ttactagtaa
    15781 caactggtca ttactacttt taccacaatg tatactagtt tatcatcatt ttgttattta
    15841 agtcttttaa attatgtaaa aacctgtgat gttcacaaaa aagacatttt taatccaata
    15901 ctaattctat agcttttatg ttatattttc aagcaagatt cagtcagatt gtgaaaaaat
    15961 taattaattt tttcgtagcc tgatttcgat taaaaatgta tccgctatca cgatcattta
    16021 aaatactatt tatagtcaga ttcttaattt tcaataagct ttttaataaa acaaaatatc
    16081 tttacgcaaa aataggacat aatcaaatat gtccttaatc gttattgata tcaatttgta
    16141 tcccaaaaat tgggttaacc attaaaaagt tattttatta gcaattttca ggattacctg
    16201 caacttttgc agttctaaat gaactaccac agccacatga agcaattgca ttaggattgt
    16261 cgatttggaa accgccaccc attaatgatt gcttaaaatc aatagtcgta ccatttaata
    16321 cgggtgcatc ttttttgtct actaatactt ttaatccaaa gtattctaag acttcatcat
    16381 tttcaccagg cgcttcttct gcacccatac cgtatgttaa accagtgcac ccgccaccat
    16441 tcactttaat ttttaaatag ccatctggca tttcatttgc tttaagcata tcttttactt
    16501 cgtaagcagc tgcttctgtt aatataactg ttggcatgat aactcctcct taaaaaatcc
    16561 aagtttcttt tatatgtgca tatatatttt gtaataattc ttccggcgaa tcaccttcaa
    16621 caatatcacc atttactaaa gcatacaacc cggctgaaca tataccacaa tgtgtcaggc
    16681 aaccatactc taacacatcg acatctgggt cattttccag ttgattaaaa acataatctc
    16741 caccttttgc catgttagag agacaaaatt ctacgatcgg attcatactt caccttctta
    16801 tttcatttgt tacaatatta tagcatttta aaactggtat tttaacatga tgtgctcaat
    16861 tagcaacaac tgatgtttct tatcccagtt atgtaatagt gccttagtta gtacaaatat
    16921 ttatttgtag tcattttcaa aaa
//
LOCUS       DALAGD010000069        16768 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.070, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000069 DALAGD010000000
VERSION     DALAGD010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16768
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.070"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(112..1419)
                     /gene="brnQ"
                     /locus_tag="O2701_001861"
     CDS             complement(112..1419)
                     /gene="brnQ"
                     /locus_tag="O2701_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCZ4293747.1"
                     /translation="MKKQVIISGLMLFSLFFGAGNLIFPPMLGHTAGQNMWIGMLGFA
                     LTGILLPFITVIVVAFYDEGVESVGNRIHPWFGFIFAVVIYMSIGAFYGIPRAANVAY
                     EIGTRHILPVHNQWTLIIFAAIFFAIVYWISLNPSKIVDNLGKLLTPLLLLMVALLSI
                     AVIFNPESALSAPKDKYITHPFISGSLEGYFTMDLVAALAFSVVIVNGYKFKGLTDRM
                     KILKYVCFSGLIAAILLGMIYFALAYVGASTAPGNFKDGTDILTYNSLRLFGSFGNLV
                     FGMTVILACLTTCIGLVNACATFTKKHVPKFSYKIFALIFSIIGFLFTTLGLEMILKI
                     AVPLLTLIYPVSIALVLISFANMFSTFRFSWAYRLATVITLIISILQILNSFNLLHGV
                     ILKSFMMLPLADIDLAWLVPFMLFAIIGFIIDVFIRRPKQATT"
     gene            2003..2893
                     /locus_tag="O2701_001862"
     CDS             2003..2893
                     /locus_tag="O2701_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HCZ4293748.1"
                     /translation="MNKISKYIAIASLSVAVTVSAPQTTNSTAFAKSSAEVQQTQQAS
                     IPASQKANLGNQNIMAVAWYQNSAEAKALYLQGYNSAKTQLDKEIKKNKGKHKLAIAL
                     DLDETVLDNSPYQGYASIHNKPFPEGWHEWVQAAKAKPVYGAKEFLKYADKKGVDIYY
                     ISDRDKEKDLKATQKNLKQQGIPQAKKSHILLKGKDDKSKESRRQMVQKDHKLVMLFG
                     DNLLDFTDPKEATAESREALIEKHKDDFGKKYIIFPNPMYGSWEATIYNNNYKASDKA
                     KDKLRKNAIKQFDPKTGEVK"
     gene            3142..4191
                     /locus_tag="O2701_001863"
     CDS             3142..4191
                     /locus_tag="O2701_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HCZ4293749.1"
                     /translation="MFLAWNEIRRNKLKFGLIIGVLTMISYLLFLLSGLANGLINMNK
                     EGIDKWQADAIVLNKDANQTVQQSVFNKKDIENKYKKQATLKQTGEIVSNGHQKDNVL
                     VFGVEKSSFLVPSLIEGHKATKDNEVLADETLKNKGFKIGDTLSLSQSDEKLHIVGFT
                     ESAKYNASSVIFTNDATIAKINPRLTGDKINAVVVRDTNWKDKKLNQELEAVSINDFI
                     ENLPGYKPQNLTLNFMISFLFVISATVIGIFLYVMTLQKTSLFGILKAQGFTNGYLAN
                     VVISQTVILALFGTAFGLLLTGVTGAFLPDAVPVKFDVLTLLVFAIVLMIVSVLGSLF
                     SILTIRKIDPLKAIG"
     gene            4204..4881
                     /locus_tag="O2701_001864"
     CDS             4204..4881
                     /locus_tag="O2701_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4293750.1"
                     /translation="MLKFENVTKSFKDGNRNIEAVKDTNFEINKGDIIALVGPSGSGK
                     STFLTMAGALQTPTSGHILINNQDITTMKQKALAKVRMSEIGFILQATNLVPFLTVKQ
                     QFTLLKKKNKNVMSNEDYQQLMSQLGLTSLLNKLPSEISGGQKQRVAIAKALYTNPSI
                     ILADEPTAALDTENAIEVIKILRDQAKQRKKACIIVTHDERLKAYCDRSYHMKDGVLN
                     LENETVE"
     gene            5089..6120
                     /locus_tag="O2701_001865"
     CDS             5089..6120
                     /locus_tag="O2701_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCZ4293751.1"
                     /translation="MDLLIGTLFLFLVLVIFTLFTYKAPNGMRAMGALANAAIATFLV
                     EAFNKYVGGEVFGIKFLEELGDAAGGLGGVAAAGLTALAIGVSPVYALVIAAACGGMD
                     LLPGFFAGYMIGYVMKYTEKYVPDGVDLIGSIVILAPLARLIAVLLTPVVNSTLIRIG
                     DIIQSSTNTNPIIMGIILGGIITVVGTAPLSSMALTALLGLTGVPMAIGAMAAFSSAF
                     MNGTLFHRLKLGDRKSTIAVSIEPLSQADIVSANPIPIYITNFFGGAIAGLIIAMSGL
                     INDATGTATPIAGFLVMFGFNHPTTIVIYGVVMAIVGALAGYLGSIVFKKYPIVTKQD
                     MINRGAVDA"
     gene            6463..7581
                     /locus_tag="O2701_001866"
     CDS             6463..7581
                     /locus_tag="O2701_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCZ4293752.1"
                     /translation="MSDSEKEILKRIKDNPFISQRELAEAIGLSRPSVANIISGLIQK
                     EYVMGKAYVLNEDYPIVCIGAANVDRKFYVHKNLVAETSNPVTSTRSIGGVARNIAEN
                     LGRLGETVAFLSASGQDSEWEMIKRLSTPFMNLDHVQQFENASTGSYTALISKEGDMT
                     YGLADMEVFDYITPEFLIKRSHLLKKAKCIIVDLNLGKEALNFLCAYTTKHQIKLVIT
                     TVSSPKMKNMPDSLHAIDWIITNKDETETYLNLKIESTDDLKIAAKRWNDLGVKNVIV
                     TNGVKELIYRSGEEEIIKSVMPSNSVKDVTGAGDSFCAAVVYSWLNGMSTEDILIAGM
                     VNAKKTIETKYTVRQNLDQQQLYHDMEDYKNGKFTKVY"
     gene            7556..8479
                     /locus_tag="O2701_001867"
     CDS             7556..8479
                     /locus_tag="O2701_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCZ4293753.1"
                     /translation="MANLQKYIEYSREVQQARENNQPIVALESTIISHGMPYPQNVEM
                     ATTVEQIIRNNGAIPATIAIIDGKIKIGLESEDLEILATSKDVAKVSRRDLAEVIAMK
                     CVGATTVATTMICAAMAGIQFFVTGGIGGVHKGAEHTMDISADLEELSKTNVTVICAG
                     AKSILDLPKTMEYLETKGVPVIGYQTNELPAFFTRESGVKLTSSVETPERLADIHLTK
                     QQLNLEGGIVVANPIPYEHALSKAYIEAIINEAVVEAENQGIKGKDATPFLLGKIVEK
                     TNGKSLAANIKLVENNAALGAKIAVAVNKLL"
     gene            8490..9710
                     /locus_tag="O2701_001868"
     CDS             8490..9710
                     /locus_tag="O2701_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCZ4293754.1"
                     /translation="MNILFAITGIAFALFVAFLFSFDRKKIDFKKTLIMIFIQVLIVL
                     FMMNTTIGLTILTALGSFFEGLINISKAGINFVFGDIQNKNGFTFFLNVLLPLVFISV
                     LIGIFNYIKVLPFIIKYVGIAINKITRMGRLESYFAISTAMFGQPEVYLTIKDIIPRL
                     SRAKLYTIATSGMSAVSMAMLGSYMQMIEPKFVVTAVMLNIFSALIIASVINPYKSDD
                     TDVEIDNLTKSTETKTLNGKTGKPKKVAFFQMIGDSAMDGFKIAVVVAVMLLAFISLM
                     EAINIMFGSVGLNFKQLIGYVFAPIAFLMGIPWSEAVPAGSLMATKLITNEFVAMLDF
                     KNVLGDVSARTQGIISVYLVSFANFGTVGIIVGSIKGISDKQGEKVASFAMRLLLGST
                     LASIISGSIIGLVL"
     gene            complement(9815..11347)
                     /locus_tag="O2701_001869"
     CDS             complement(9815..11347)
                     /locus_tag="O2701_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HCZ4293755.1"
                     /translation="MKEVGFGTLNWVAVIIYLLAMLFIGVYFTKRASQSTNSFFTASG
                     RLPSWVVGFSIYATTLSAITFMSTPEKAFLTDWSYIAGNIAIVAIIPLLIYFYVPFFK
                     KLKVTSAYEYLEARFGPSIRVIGSLLFVVYHLGRVAIVIYLPTLAITSVSDMNPYIVA
                     SLVGLLCILYTFLGGFEGVVWSDFIQGVILLGGALVIIILGVVNIKGGFGTVFADAIE
                     HKKLISADNWKLNTAAAAIPIIFLGNIFNNLYQYTASQDVVQRYQASDSLKETNKSLW
                     TNGILALISAPLFYGMGTMLYSFYTHEAVLPKGFNTSSVVPYFILTEMPPFVAGLLIA
                     AIFAAAQSTISSSLNSISACISIDIKQRFFGKGSERHEVNFARFIIIIAGIFGFGMSL
                     YLIASNSNDLWDLFLFVTGLFGVPLAGVFAVGIFTKRTNTFGVICGLILGIIFAYVYN
                     GVGKGNSPFYVSTISFTVAFVFAYILSFIVPSKHKKDITGLTIFEKDKPSTYISKTAT
                     KK"
     gene            complement(11387..12268)
                     /locus_tag="O2701_001870"
     CDS             complement(11387..12268)
                     /locus_tag="O2701_001870"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCZ4293756.1"
                     /translation="MNKDLKGLYAALLVPFDENGQVNEQGLKQIAQNAIETEELDGLY
                     VNGSSGENFLLNTEQKKQVFKVAKEAVGDKVKLIAQVGSLDLNEAIELGKYATELGYD
                     ALSAVTPFYYPFTFEEIRDYYFDIIEATQNNMIIYAIPDLTGVNISIEQFSELFNHEK
                     IVGVKYTAPNFFLLERIRKAFPDKLILSGFDEMLVQATISGVDGAIGSTYNVNGRRAR
                     KIFDLARQGQIQEAYQLQHDSNDIIETVLSMGIYPTLKEILRHRGIDAGLPKRPFKPF
                     NEAHRQTLDQLIAKYDL"
     gene            12426..13286
                     /locus_tag="O2701_001871"
     CDS             12426..13286
                     /locus_tag="O2701_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCZ4293757.1"
                     /translation="MYYIAIDIGGTQIKSAVIDKQLNMFDYQQISTPDNKSELITDKV
                     YEIVTGYMKQYQLIQPVIGISSAGVVDEQKGEIVYAGPTIPNYKGTNFKRLLKSLSPY
                     VKVKNDVNAALLGELKLHQYQAERIFCMTLGTGIGGAYKNNQGHIDNGELHKANEVGY
                     LLYRPTENTTFEQRAATSALKKRMIAGGFTRSTHVPVLFEAAEEGDDIAKQILNEWAE
                     DVAEGIAQIQVMYDPGLILIGGGISEQGDNLIKYIEPKVAHYLPKDYVYAPIQTTKSK
                     NDAALYGCLQ"
     gene            complement(13564..14364)
                     /locus_tag="O2701_001872"
     CDS             complement(13564..14364)
                     /locus_tag="O2701_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCZ4293758.1"
                     /translation="MKFENRVQRYQHLFTKTDKRIVNYIRQNGYSDAFSTINSLAHAI
                     GTSPATMTRFSHKLDYENFQDLKFNIQQEMTETVIENSPIIQRIHKYHQQIIQQTGEF
                     IDNDIIQTFIDKLQSSRHILFAGLGSSGLSATEFYYRMIRMGLKGNVTTDSHLMKISA
                     SLLSHSDMFIAMSNSGNTSELISAAEVAKSHGAYVVAITNFEGSKLTDCADLVLLTTD
                     QSRNTDHQFINTQIATLFLIDIVSYHLLENTNLSQTYQHTKSIILDNK"
     gene            14504..15172
                     /locus_tag="O2701_001873"
     CDS             14504..15172
                     /locus_tag="O2701_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCZ4293759.1"
                     /translation="MLPHGLIVSCQALPDEPLHSSFIMSKMALAAYEGGAVGIRANTK
                     EDILAIKETVDLPVIGIVKRDYDHSDVFITATSKEVDELIESQCEVIALDATLQQRPK
                     ETLDELVSYIRTHAPNVEIMADIATVEEAKNAARLGFDYIGTTLHGYTSYTQGQLLYQ
                     NDFQFLKDVLQSVDAKVIAEGNVITPDMYKRVMDLGVHCSVVGGAITRPKEITKRFVQ
                     IMED"
     gene            complement(15299..16612)
                     /locus_tag="O2701_001874"
     CDS             complement(15299..16612)
                     /locus_tag="O2701_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCZ4293760.1"
                     /translation="MNQYHSNAQQPSAWRFFVYSLVGILCFFIPFTINGNNTIFVDHV
                     HLAIRSIIGPLMPYVALIMILIGTALPIVRRTFMTSITNLVITLFKVAGAMIGIMYVF
                     KIGPSILFKANYGPFLFEKLMMPLSILIPVGAIALSLLVGYGLLEFVGVYMEPIMRPI
                     FKTPGKSAVDAVASFVGSYSLGLLITNRVYKQGMYNKREATIIATGFSTVSATFMIIV
                     AKTLGLMPHWNLYFWITLVITFVVTAITAWLPPISNESTEYYNGQEGEQEVAIEGSRL
                     KTAYAEAMKQNALTPSLVKNVWDNLKDGLEMTVGILPSILSIGFLGLIVANYTPFIDW
                     LGYIFYPFIYIFPIADQALLAKASAISIVEMFLPSLLVTKAAMSTKFVVGVVSVSAII
                     FFSALVPCILATEIKIPVWKLIIIWFLRVALSLLITIPVALLIFG"
BASE COUNT         5844 a         2595 c         3166 g         5163 t
ORIGIN      
        1 tcatttattt taaaataatc aatagacaga cggtatcact gaaaagtcat gaatttcata
       61 taaaaaagcc tagtaacgat gggtcaatca ttactaggca gtgagcattt attaagttgt
      121 cgcttgtttc ggacggcgta taaatacatc gattatgaaa ccgataatag caaagagcat
      181 gaatggtaca agccaagcta aatcgatatc tgctaaaggt aacatcataa acgatttcaa
      241 aataacaccg tgtaataagt tgaaactatt tagtatttgt aaaattgaaa taatcaatgt
      301 aataacagtt gcgagtcgat aggcccaact gaatctgaat gtgctaaaca tgttagcaaa
      361 tgatatgagt acaagtgcaa tcgacacggg atatattaaa gtcaataatg ggacagcaat
      421 ttttaaaatc atttctaaac caagtgttgt aaataagaac cctatgatag agaaaataag
      481 tgcgaatatt ttataagaaa acttaggtac gtgtttctta gtaaatgtgg cgcaagcatt
      541 gacgagtcct atacatgttg ttaggcatgc aaggataacc gtcattccaa atacgaggtt
      601 accgaacgaa ccaaataatc gtaatgagtt gtacgtcaat atatctgtac catctttaaa
      661 gtttcctgga gctgttgatg ccccaacgta tgcaagtgca aagtaaatca ttccaagtaa
      721 tatggctgca ataagacctg aaaagcagac atattttaaa attttcatgc gatctgtgag
      781 gcctttaaac ttatagccat tgacaatgac tacggaaaaa gctaacgcag caacaagatc
      841 cattgtaaaa tagccttcca aacttcctga aatgaaagga tgtgttatat atttatcctt
      901 aggtgcactt agtgcagatt cagggttgaa aatgacagca atacttaata gagcgaccat
      961 taatagtaat aacggtgtta ataatttacc taaattatca acgattttcg atggatttaa
     1021 actaatccag taaacgatgg caaaaaagat tgctgcgaat ataattaaag tccattggtt
     1081 atgcacaggt aaaatgtgtc ttgtaccaat ttcgtacgcg acatttgcag cacgtggaat
     1141 accgtaaaat gctccgatag acatgtaaat cacgacagca aaaataaacc cgaaccatgg
     1201 atgtatacga ttgcctacac tttcaacacc ttcatcataa aatgcaacaa caataacagt
     1261 aataaagggg agtaatatgc ctgtaagggc aaagcctagc ataccaatcc acatattttg
     1321 acccgctgta tggccaagca tgggcgggaa tattaaattt ccggctccaa aaaatagtga
     1381 aaataacatg aggcccgaaa taataacttg ttttttcaat gtaatttcct tcctaaaact
     1441 agatattcat aataatttaa aaaatctgaa aaataaaaac gttcttactt tatctttaaa
     1501 tgacaatact atgattctat tattttataa aaaagattgc aataataacg tcttgctatt
     1561 attggataat gacaattaaa gtgtcgtttt tatgaattaa aataggtgaa agtaagttgg
     1621 aaatacgttg gagcgttgtg atttttatga gaaattgtat gagtgaggac tgctatttat
     1681 aaaaggtaaa ctatggatga gtttatggtt gttccaatag gtaaataaga gatagcacac
     1741 tgtacacata atgatacgtg gatgagtgaa tatgtctgat aggaaggcct agtcactcaa
     1801 atgaatttaa gtttattacg tgataaatca caaatctctc tcatgtgata ggtctcccat
     1861 taaatcatgc ttatatgaaa tgttcacata tttgttagct tttcaagaaa taatattaaa
     1921 tatcttttca tagtatagca atattaaata aatgcattta taattttaac tgtattttaa
     1981 atattaatca tgaggtgata agatgaataa aatttcaaag tatattgcaa tagcatcatt
     2041 atcggtagcg gttacagttt cagcaccaca aacgacaaat tctacagcgt ttgccaaaag
     2101 ttctgctgaa gttcaacaaa cgcaacaagc ttctatacca gcatcacaaa aggcgaatct
     2161 tggtaatcaa aatattatgg cagtggcttg gtatcaaaat tcagctgaag caaaagcatt
     2221 atatttacaa ggttataaca gtgcaaagac acagttagat aaagagatta aaaagaataa
     2281 aggtaaacat aagttagcta ttgctttgga tttagatgaa acagttttag ataattctcc
     2341 atatcaaggc tatgcatcaa tacataataa acctttccca gaaggttggc atgaatgggt
     2401 acaagctgct aaagctaaac ctgtctatgg cgcaaaagaa ttcttgaaat atgctgacaa
     2461 aaaaggtgtc gatatctact atatttctga tagagataaa gaaaaagatt taaaggcaac
     2521 acaaaagaac ttaaaacaac aaggtatccc tcaagctaag aagagtcata ttttactaaa
     2581 aggtaaagat gataagagta aagaatcacg cagacaaatg gttcaaaagg atcataaact
     2641 tgtcatgcta tttggagata atttattaga ctttacagat ccaaaagaag ctacagctga
     2701 atctcgtgaa gcattaattg aaaaacataa agacgatttc ggtaagaaat atatcatttt
     2761 ccctaaccca atgtatggta gttgggaagc tacgatttac aacaataact ataaagcaag
     2821 tgacaaagca aaagataaat tacgtaaaaa tgctattaag caattcgatc ctaaaacagg
     2881 cgaagttaaa taatatatga attggacgtc tacatgtact taaaggtata tgtaggcgtt
     2941 tttaaattga tccttaattt taaatgtacc gtacaaaaat aatagacaat taattgcatt
     3001 ttccataaat ttgcttttaa tataaaaagt tcgggtataa tgaaggataa gtgtatgtat
     3061 aacattacag agcgcaacac gacgtacgtg catctaagaa tataaacaat gtttaaatca
     3121 tagtagtagg gagagaaatg catgttttta gcttggaatg aaatacggcg caacaaattg
     3181 aagtttggac taattattgg tgtgttaacg atgattagtt acttgctatt tttattatct
     3241 ggattggcga atggtcttat caatatgaat aaagaaggca ttgataagtg gcaagcagat
     3301 gccattgttc taaataaaga tgccaatcaa actgtgcaac aatctgtttt taacaagaaa
     3361 gatattgaaa ataaatacaa gaagcaagct actttgaagc aaacagggga aattgtgtct
     3421 aatggccatc aaaaagacaa tgttttagtg ttcggtgttg aaaagtcatc atttttagtt
     3481 ccgagtttaa tagaagggca taaagcgact aaagataatg aagtgttagc tgatgaaacg
     3541 cttaaaaata aaggatttaa aattggtgac acattatcac tatctcaatc agatgaaaaa
     3601 ttgcatatcg taggttttac agaaagtgca aaatataatg cgtcatcagt cattttcacg
     3661 aatgacgcta ccattgccaa gatcaatcct agattgactg gagataaaat taatgcagtt
     3721 gttgtacgtg atacaaattg gaaagacaaa aaattaaacc aagagcttga agcggtaagt
     3781 attaatgact ttattgaaaa tttaccaggt tataaaccac agaacttaac attaaacttt
     3841 atgatttcat tcttatttgt catttcagct acagttatag gcattttcct atatgtcatg
     3901 acattacaaa agacgagttt atttggcata ttaaaagctc aaggatttac gaatggctat
     3961 ttggcgaatg tggtaatttc gcagacggtc atattagcac tatttggtac ggcatttggc
     4021 ttactgttaa caggcgttac aggtgcattt ttacctgatg cagtacctgt caaattcgat
     4081 gtactaacat tgctcgtatt tgcaattgtg ttaatgattg tctctgtatt aggaagttta
     4141 ttctccattt taacaattag aaaaatagat ccgttaaagg cgattgggta ggaggtgtag
     4201 caaatgttga aatttgaaaa tgtaacaaag tcatttaaag atgggaatcg taacattgaa
     4261 gcggttaaag atacaaattt tgagataaat aaaggtgata ttatagcatt ggttggacct
     4321 tctggctctg gtaaaagtac atttctaact atggcaggtg ctttacaaac accgacatct
     4381 gggcacattt taatcaataa ccaagatatt acgacaatga agcaaaaagc attggcaaaa
     4441 gttagaatgt ctgaaatagg ttttatttta caagctacaa accttgtacc atttttaacg
     4501 gtaaagcaac aatttacatt attgaaaaag aaaaataaga atgttatgtc taatgaagac
     4561 tatcagcaac ttatgtcaca attaggtcta acttcattgc ttaataagtt accttcagaa
     4621 atttcaggtg gtcagaaaca acgtgtggcg atagccaaag cgttatatac gaatccgtcg
     4681 attattttag cggatgaacc taccgcggcg ttagatactg aaaatgcgat tgaagtcatt
     4741 aaaattctac gtgatcaagc caaacaaaga aagaaagcat gtattattgt tacacatgat
     4801 gaacgactta aagcatattg tgatcgttca tatcatatga aagatggcgt ccttaatctt
     4861 gaaaatgaaa cagtagaata gttttattaa gccggtacat catgtgccgg tatttttatg
     4921 tttatgtatt atttgaataa actttcacat tcaattaata ataattatta tcgaaaatca
     4981 gaaatattcc gtgaaatata atattttttg tagtaaaatg gcctctaagt attcaatatt
     5041 taaatatggg gattgaatat aaaattatcg taatgggggt caatggttat ggatttattg
     5101 ataggtactt tatttttatt tttggtctta gtgattttta cattatttac atataaagcg
     5161 cctaatggta tgcgtgccat gggagcatta gctaatgcag caatcgcaac atttttagtg
     5221 gaagcattta ataaatatgt tggtggcgaa gtattcggta ttaaattttt agaagagcta
     5281 ggagacgctg cgggaggtct aggtggtgtc gctgccgctg gattaacagc attagctatc
     5341 ggtgtgtcac cagtatatgc attagttata gcagccgcgt gcggtggtat ggatttatta
     5401 ccaggtttct ttgcgggtta tatgattgga tatgtgatga aatatacaga gaaatatgtg
     5461 ccggatggtg tcgacttaat tggatcgatt gtcatcttag cgccattagc tcgtcttatt
     5521 gcagtattat taacgccagt agtgaatagt acattgattc gaattggtga tattatccaa
     5581 agtagtacga atacgaatcc aattatcatg ggtatcattt taggtggtat tattacggtt
     5641 gtcggcacag cgccattgag ttcaatggca ttgacagcat tattaggttt aacgggtgta
     5701 cctatggcta ttggtgccat ggcagcattt agttcggcat ttatgaatgg gacgctattc
     5761 catcgcttaa aattaggtga tcgtaagtct acgattgcag taagtattga acctttatca
     5821 caagcagata ttgtatcagc caatccaatt ccaatctata ttacaaattt ctttggtggt
     5881 gcgattgctg gtttaattat tgctatgtca ggtttaatta acgatgcgac aggtacagct
     5941 acaccgattg caggattttt agttatgttt ggatttaatc atccgacgac aattgtgatt
     6001 tatggtgtag taatggcgat tgtaggtgcg cttgcaggtt atcttggttc aattgtattt
     6061 aaaaaatatc caattgttac taagcaagac atgattaatc gaggtgcagt agacgcatag
     6121 catcatcata ttgaatagta aaaacaaata aaacatagta acgtgattca gtcgatgtaa
     6181 cagtcgataa tgagtcacgt ttttttatag aaaaatacaa gacataaaaa tgtcataatt
     6241 tattgtcgac aaatatcata ctgtataaac atttatcatt ttctcaagta ccttttacac
     6301 gatggaatga acttactttt tacgaaatta tgcgtatttt ataaacaaat atcattgata
     6361 taacggtaaa tgtaagcgtt tacaacagaa ataacagcat gctacgatat ttttgtaaat
     6421 tcactgattc aagtatttta agtcaatatg aggagggatg ttatgagcga ttctgagaaa
     6481 gaaattttaa aaagaattaa agataatccg tttatttcac aacgtgaact tgctgaggca
     6541 attggattat ctagacccag cgtagcaaac attatttcag gattaataca aaaggaatat
     6601 gttatgggaa aggcatatgt tttaaatgaa gattatccta ttgtttgtat tggcgcagcg
     6661 aatgtagatc gtaagtttta tgtgcataaa aatttagttg cagaaacatc aaatcctgta
     6721 acgtcaacac gctctattgg tggcgtagca agaaatattg ctgagaactt aggtaggctt
     6781 ggcgaaacgg tcgctttttt atctgctagt ggacaagata gtgaatggga aatgattaaa
     6841 cgattgtcca caccatttat gaatttggat catgttcaac aatttgaaaa tgcgagtaca
     6901 ggttcatata cagctttaat tagtaaagaa ggcgacatga catatggctt agcagatatg
     6961 gaagtgtttg actacattac gcctgaattt ttaattaagc gttcacactt attgaaaaag
     7021 gctaagtgca ttattgtaga tttgaattta ggcaaagagg cattaaactt cttatgtgcc
     7081 tataccacga aacatcaaat caaattagtt atcaccacgg tttcttcccc aaaaatgaaa
     7141 aatatgcctg attcattaca tgctattgat tggattatca cgaataaaga tgaaacagaa
     7201 acatacttaa atttaaaaat agaatctact gatgatttaa aaatagctgc taaacgctgg
     7261 aatgatttag gtgttaaaaa tgttattgtg acaaatggcg tgaaagaact catttatcga
     7321 agtggtgagg aagaaatcat taagtcagtt atgccatcaa atagtgtgaa agatgttaca
     7381 ggtgcaggcg attcattctg tgctgcagta gtgtatagct ggttaaatgg gatgtctact
     7441 gaagatatat taattgctgg tatggttaac gcaaagaaaa cgatagaaac gaaatataca
     7501 gttaggcaaa acctagatca acagcaactt tatcacgata tggaggatta taaaaatggc
     7561 aaatttacaa aagtatattg agtattctcg agaagttcag caagcacggg agaacaatca
     7621 accgattgta gcattagaat caacaattat ttcgcatggt atgccgtacc cacaaaatgt
     7681 tgaaatggca acaacagtag agcaaattat caggaataat ggtgccattc cagcaaccat
     7741 agccattata gatggcaaaa ttaaaattgg tttagaaagc gaagatttag aaatactggc
     7801 aactagtaaa gacgttgcta aagtatctag aagggattta gcagaagtta ttgcgatgaa
     7861 gtgtgttggt gctactactg tagcgacgac gatgatatgt gctgcaatgg ctggtattca
     7921 attttttgtt acaggaggta ttgggggcgt ccataaaggt gcagaacata cgatggacat
     7981 ttcagcagac ttagaagaac tgtctaaaac aaatgtcact gttatctgtg caggtgccaa
     8041 atcaatttta gacttaccta agacgatgga gtatttagaa acaaaaggcg ttccagttat
     8101 tggatatcaa acgaatgaat tgccagcatt cttcactcgc gaaagcggtg ttaagttaac
     8161 aagttcggtt gaaacgccag aacgacttgc tgacattcat ttaacaaaac agcagttaaa
     8221 tcttgaaggt ggcattgttg ttgctaatcc aattccatat gagcatgcct tatcaaaagc
     8281 atatattgag gcaatcataa atgaagctgt tgttgaagcg gaaaatcaag gtattaaagg
     8341 taaggacgcc acaccgttct tgttagggaa aattgtagaa aaaacgaatg gtaaaagttt
     8401 agcagcaaat ataaaacttg ttgaaaacaa tgcggcgttg ggtgctaaaa ttgctgtcgc
     8461 tgttaataaa ttattgtagg tgatgataca tgaatatttt attcgctatc acagggatag
     8521 catttgcact atttgttgcg tttttattca gttttgatcg taaaaaaata gacttcaaaa
     8581 agacgttaat aatgatattt attcaagtgt tgatcgtgtt atttatgatg aacacaacga
     8641 ttggtttgac aattttaact gcactaggtt cattttttga agggctaata aatattagta
     8701 aagcaggcat aaattttgtt tttggagata tacaaaataa aaatggcttt acgttctttt
     8761 taaacgtatt actgccatta gtttttattt ctgtattaat aggcatcttt aattatatta
     8821 aggtattacc atttattatc aaatatgtag gtatcgctat taataaaata actagaatgg
     8881 ggcgcttaga aagttatttt gctatttcaa cagcaatgtt tgggcaacca gaagtatatt
     8941 taacaataaa agatattatt ccaagattat ctagagcgaa attatataca attgcgacgt
     9001 ctggtatgag tgctgttagt atggcaatgc taggttcata tatgcagatg attgaaccca
     9061 agttcgtagt tacagcagta atgttaaata tttttagtgc gcttatcatc gccagtgtaa
     9121 tcaatcccta taaatctgat gatactgatg ttgaaattga taacttaacg aaatccacag
     9181 aaactaaaac attgaatgga aaaacaggaa aacctaagaa agttgccttt ttccaaatga
     9241 ttggtgatag tgcgatggat gggtttaaaa tcgctgttgt agtagccgta atgttgttag
     9301 catttatttc attaatggaa gcaattaata tcatgtttgg tagtgttggt ttgaacttta
     9361 aacagcttat tggctatgtg tttgcaccaa tcgcattctt aatggggatt ccatggagcg
     9421 aagctgttcc agctggctct ttaatggcga ctaaattaat tacaaatgag tttgtagcaa
     9481 tgcttgattt taaaaatgtc ctgggtgatg tatcagctcg aacacaaggt atcatttcag
     9541 tttacttagt aagcttcgct aattttggta cggttggtat catcgtaggt tcaattaaag
     9601 gcattagtga taaacaagga gaaaaagttg catcctttgc aatgaggttg ctacttggtt
     9661 caactctagc ttcaatcatt tcaggatcaa tcattggctt agtattgtaa atgaatcgaa
     9721 gtacctaaat taaattcatg gcaaagctaa accccgtcac caagttggcg caacagcgca
     9781 tcataactta gtgacggggt tttatcataa caatctactt tttcgtagcc gtttttgaaa
     9841 tgtatgttga tggtttatct ttttcaaaaa ttgttaatcc cgttatatct tttttatgtt
     9901 ttgaagggac aatgaagcta agtatataag caaagacaaa agcaactgta aatgaaatgg
     9961 tagatacata gaaaggtgag ttacctttgc caacaccatt atagacataa gcaaagatga
    10021 tacccaatat taatccacaa ataacaccga atgtattcgt acgtttagtg aaaataccaa
    10081 ctgcaaatac accagccaat ggaacgccga ataatccagt cacaaacaag aataaatccc
    10141 ataagtcatt tgaattagaa gcaattaagt atagtgacat tccaaaaccg aaaatacctg
    10201 caatgataat aatgaaacgt gcaaagttaa cttcgtgtcg ctcgctacct tttccgaaga
    10261 agcgttgctt aatgtcgatt gaaatacaag cagatataga atttaaacta gatgaaatgg
    10321 tagactgtgc agcggcgaaa atggctgcaa taagtaatcc tgctacaaat ggtggcatct
    10381 cagtcaaaat gaaatatggc actacagatg atgtattgaa gccttttggt aaaacagctt
    10441 catgtgtata aaatgaatac agcattgtac ccataccata aaataagggt gctgaaatta
    10501 aagctaggat accatttgtc cataacgatt tatttgtttc ttttaaacta tcagaagctt
    10561 gataacgctg cacgacgtct tgactcgctg tgtattgata caagttgttg aaaatatttc
    10621 ctaggaaaat aattggaatg gcagctgccg cagtatttag tttccaattg tctgcactaa
    10681 ttaatttttt gtgctcaatc gcatctgcaa agacagtgcc gaaaccgcct ttaatgttca
    10741 caacacctag aataataata actaaagcgc cgcctaataa aatgacgcct tgaatgaaat
    10801 cactccaaac cacaccttcg aaaccaccta aaaatgtata taaaatacat agtaaaccaa
    10861 cgagtgatgc aacgatataa gggttcatgt ctgatacaga tgtgattgct aatgttggta
    10921 agtagataac aattgcaaca cgccctaaat ggtaaacgac aaataataat gagccaatga
    10981 cacgtatgct agggccaaat ctagcttcta aatattcata tgcagatgtt acctttaact
    11041 ttttaaagaa agggacatag aaataaataa gtaatggaat aattgcgacg atagcaatgt
    11101 taccagcgat atatgaccaa tctgttaaaa atgctttctc tggtgtcgac ataaatgtaa
    11161 tcgcacttaa cgtagtagca taaattgaaa agccaactac ccaagatggc aagcgaccac
    11221 ttgcggtaaa gaaactattg gtactttggc tcgcgcgctt ggtaaaataa acgccaatga
    11281 acaacatagc tagtagataa atgataacgg caacccagtt tagtgtgcca aatccaactt
    11341 ctttcatggg caacatcccc tttacaatgt attgattctt tgatgtctat aaatcgtatt
    11401 ttgcaatgag ttgatctaat gtttgtcgat gtgcttcgtt aaaaggtttg aaaggtcttt
    11461 tcggtaatcc tgcatcaatg ccacgatgac gtaatatttc tttcaatgtt ggataaatcc
    11521 ccattgataa cactgtttcg ataatgtcgt ttgaatcatg ttgcagttgg taagcttctt
    11581 gaatttgacc ttgtcgtgct aagtcgaaga tttttcttgc acggcgacca ttaacgttat
    11641 atgtagaacc aattgcacca tctacgccag aaatcgtagc ttgaactaac atttcatcaa
    11701 agccagataa gattaatttg tctgggaatg cttttctaat acgttcgagt aggaagaagt
    11761 ttggcgctgt atatttaaca ccaacaattt tttcatgatt aaatagctcg ctgaattgtt
    11821 caatagaaat attcacacct gttaaatctg gtattgcata aataatcata ttgttctgag
    11881 ttgcttcgat aatatcgaaa tagtaatctc taatttcttc aaaagtaaat ggatagtaga
    11941 atggtgttac ggcagaaagt gcatcataac cgagttctgt ggcatatttt ccaagttcaa
    12001 tggcttcatt taaatctaac gaacctactt gagcaatcaa tttcacttta tccccaactg
    12061 cctctttggc aaccttgaaa acttgcttct tctgctctgt atttaataaa aagttttcgc
    12121 ctgagctacc atttacataa agaccgtcta attcttcagt ttcaatggca ttttgagcaa
    12181 tttgtttaag tccttgttca tttacttgac cattttcatc aaaaggaacg agtaacgctg
    12241 catataaacc ttttaaatct ttgttcatta tgaagtccct ccaaaaatca tttgataata
    12301 tagtttacag ctataattgt aaacgctatc ataaaatgta acaatatctt tttgaaaatt
    12361 gtagtcatat ttatgtataa ttaatgaaaa tgtttttcaa aatcaataga aatggagtga
    12421 gtaaggtgta ttacatcgca atcgatattg gaggcactca aattaaatcg gcagttattg
    12481 ataagcaatt gaatatgttt gactatcaac aaatatcaac gccggacaac aaaagtgagc
    12541 ttattactga caaagtatat gagattgtaa caggatatat gaagcaatat cagttgatcc
    12601 aacctgtcat aggtatttca tcagcaggcg ttgttgatga acaaaaaggc gaaattgtat
    12661 acgcagggcc aaccattccg aattataaag gtactaattt taagcgatta ttaaaatcac
    12721 tgtctcctta tgtcaaagta aaaaatgatg taaacgctgc attactaggc gaattgaaat
    12781 tacatcaata tcaagcagaa cggatctttt gtatgacgct tggtacaggc attgggggtg
    12841 cgtacaagaa taatcaaggt catattgata atggtgagct tcataaggca aatgaagttg
    12901 ggtatttatt gtatcgtcca actgaaaata caacgtttga gcaacgtgct gcaacgagtg
    12961 cattgaaaaa gcgcatgatt gccggaggat ttacgagaag cacacatgtg ccagtattgt
    13021 ttgaagcagc tgaagaaggt gatgatattg caaaacaaat attgaatgag tgggcagaag
    13081 atgtagcaga agggattgcc caaatacagg tcatgtatga tccagggctt atattaattg
    13141 ggggcggtat atctgaacaa ggagataatc tcattaaata tatcgagccg aaagttgcac
    13201 actatttacc aaaagactat gtttatgcac caatacaaac gactaagagt aaaaatgatg
    13261 cagcattata tggctgtttg caatgatagt tgaaagaagg agtcattcta aaatagaatt
    13321 tgaaaccgtt acgagagatg agagctgttg ttagttccac acatcacact ctatctagga
    13381 ccaatctaaa ctatatcaac caacagtgtg ccacgggcaa attaaattga agaagctgag
    13441 atattaaaat tttagaaaat gtaaaaaaat atttggtatt gaaattaaaa aagcacctag
    13501 caactcgttg ggacaatcac gatgattgtc tacagttgca ggtggatttg aatatactac
    13561 tagttatttg ttgtctagga taatagattt agtatgttga taagtttgac tcagattcgt
    13621 attttctaat aaatgataac tcacgatatc gattaaaaag agtgtcgcaa tttgtgtgtt
    13681 gataaattga tggtcggtat tacgcgattg atccgttgtt aaaagtacta aatctgcaca
    13741 atctgtaagt ttactacctt caaaatttgt gatggcaacg acatatgcac catgagattt
    13801 ggcgacttcc gctgcagaaa ttaattccga agtattacca ctatttgaca tagcaataaa
    13861 catatccgaa tgagatagta gggatgccga tattttcatt aaatgtgaat cggtagtaac
    13921 attacctttt agccccatac gaatcatacg ataataaaat tcagtcgctg ataaaccaga
    13981 gctacctagt ccagcaaaga gtatatgtcg acttgattga agtttgtcga taaaggtttg
    14041 gataatgtcg ttatcaataa attcaccagt ttgttgaatg atttgttgat gatatttatg
    14101 aattctttga ataattgggc tattttcaat aactgtctct gtcatttctt gttgaatatt
    14161 aaattttaaa tcttggaaat tctcataatc cagcttatga ctaaagcgtg tcatcgttgc
    14221 tggtgatgta ccaatcgcat gggctaagga gttaatcgtt gaaaaggcat cgctataacc
    14281 attttgtctt atataattga cgatgcgttt atcagttttt gtaaataaat gttgataacg
    14341 ttgaacacga ttctcaaatt tcattgtgtc accccttcat cttaatgatt actattatat
    14401 atgaaaaata ttttcaagat agtaaaaagc attgataaaa attatcttaa tgatatattg
    14461 taaatgactt tacgtgaaaa aacgacttat ggagtgagga ataatgttac cacatggatt
    14521 aatagtatct tgtcaggcac taccagatga accattgcat tcatctttta ttatgtcgaa
    14581 aatggcatta gctgcgtatg aaggtggtgc tgttggtatt cgcgcaaata ctaaggaaga
    14641 cattttagca attaaagaaa cggtagattt accagttatt ggcattgtga aacgtgacta
    14701 tgatcactca gatgttttca ttactgcaac gtcaaaagaa gttgatgaac tgatagaaag
    14761 ccaatgtgaa gtcattgcat tggatgcaac gttacagcaa cgtccgaaag aaacgttaga
    14821 cgaattagta tcatatatta gaacacatgc accgaacgtt gaaatcatgg ctgatatcgc
    14881 gaccgttgaa gaagctaaaa atgccgcacg acttggcttt gattatattg gcacgacgtt
    14941 acatggctat actagttata cgcaaggaca attactttat caaaatgact tccaattttt
    15001 aaaagatgta ctacaaagtg ttgatgcaaa agttattgcg gaaggtaatg tcattacacc
    15061 ggatatgtat aaacgtgtga tggacttagg cgttcattgt tcagtcgttg gtggtgcgat
    15121 aacacgacca aaagaaatta cgaaacgttt tgttcaaatt atggaagatt aaatgataac
    15181 gataaaaaaa cgagatgacc atcattaatt aaaggcacct aattatctta ggtggctgaa
    15241 tgaatgtaat gggttcatct cgttttgttt gtttatgata gtgattttat tttcaacttt
    15301 atccaaaaat aagtaaagcg acggggatgg tgattaatag cgacaacgcc acgcgtaaaa
    15361 accaaatgat gatgagtttc cagacaggta ttttaatttc agttgctagt atacatggca
    15421 ctaatgctga gaaaaagata atggctgata cgcttactac accgacgaca aatttagtac
    15481 tcattgcagc tttagttact aacaaagatg gtagaaacat ctctacaata gaaatcgctg
    15541 acgcttttgc tagtaaagcc tgatcagcaa ttgggaaaat ataaataaat ggatagaaga
    15601 tatagccaag ccaatcaatg aatggtgtat agttcgctac aatcagtcct aaaaaaccaa
    15661 tcgataatat agaaggtaaa ataccaacag tcatttctaa accgtctttc aaattgtccc
    15721 aaacgttctt cacgagagat ggtgttaatg cattttgttt catcgcctct gcatatgcag
    15781 ttttcagtct gcttccttca atagcaactt cttgttctcc ttcttgtccg ttataatatt
    15841 ctgttgattc attgctgatt ggcggtagcc atgcagtaat tgcagtcacg acaaatgtga
    15901 tgactaaagt tatccaaaag tataaattcc aatgcggcat taatcctaaa gttttagcaa
    15961 cgataatcat aaaagttgct gaaactgttg aaaagccagt cgcaataatc gtggcttctc
    16021 gtttgttgta catcccttgc ttatagacac gattagtaat caataatcct aaggaataac
    16081 tgccgacaaa cgaagccact gcatcgacag cggattttcc tggtgtttta aaaataggtc
    16141 tcataatagg ctccatataa acaccgacaa attctaataa gccatagccc actaataaag
    16201 aaagcgcaat tgcacctact ggaattaaga tacttaatgg catcattaat ttttcaaaca
    16261 aaaacggacc atagttagct ttaaatagta ttgatggacc gattttaaat acatacatta
    16321 taccgatcat tgcacctgca actttaaata atgtaatgac caagtttgtg attgaagtca
    16381 taaaagtacg tctcactatt ggtaacgctg taccaattaa aatcataatc agtgcaacat
    16441 agggcataag tggacctatg attgagcgaa tggctagatg aacatgatcg acgaaaatag
    16501 tgttgttacc attaatcgta aaaggaataa agaaacatag tatgcccact aaactataga
    16561 caaaaaaacg ccatgcactt ggttgttgtg cattagaatg atattgattc attaaagcaa
    16621 cccctttgtt taaatgaata cacaaaactg tatgatgcat cttcccctta atgagatgaa
    16681 tcattatttt aatttagaaa aatctgaaaa cttactataa ttgtatagtt tgaattattt
    16741 tcataccaat acaaattaac taattata
//
LOCUS       DALAGD010000070        16510 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.071, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000070 DALAGD010000000
VERSION     DALAGD010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16510
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.071"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            469..1335
                     /locus_tag="O2701_001875"
     CDS             469..1335
                     /locus_tag="O2701_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001594836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCZ4293761.1"
                     /translation="MNPKVKGIIAILISAIGFSFMSVFFRLAGDLPVFQKSLARNLVA
                     MFIPLFFIYKYHQPMFGRLSSQPLLITRSTLGLIGVLLNIYAIDHMVLSDADSLMKLN
                     PFWTILLSIVFLHEKVRKYQITAMIIAILGMLLIVKPEFSSSMIPSLAGLFSGIFAAS
                     AYTCVRALSTREAPYTIVFYFSLFSVIVLIPFTAYTYEPMSQMQILYLLGAGLAAAVG
                     QIGVTLAYSFAAAKDISIFTYASIIFTAILGFILFGESPDFYATLGYVVIIGASYYMF
                     EKARRDAKIIKK"
     gene            1355..1855
                     /gene="queF"
                     /locus_tag="O2701_001876"
     CDS             1355..1855
                     /gene="queF"
                     /locus_tag="O2701_001876"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCZ4293762.1"
                     /translation="MAHGRQQDELQDITLLGNQDNTYNFDYRPDVLESFDNKHQGRDY
                     FVKFNCPEFTSLCPITGQPDFATIYISYIPNVKMVESKSLKLYLFSFRNHGDFHEDCM
                     NIIMNDLIELMDPHYIEVWGKFTPRGGISIDPYTNYGRPNSKYEKMAEHRLMNHDLYP
                     EKIDNR"
     gene            2196..3701
                     /locus_tag="O2701_001877"
     CDS             2196..3701
                     /locus_tag="O2701_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HCZ4293763.1"
                     /translation="MTQQNSHGNQIQDIPQTGFFGHPRGLGVLFFVEFWERFSYYGMR
                     ALLIFYMYFAVTDNGLGIDKTTAMSIMSVYGSLIYMTSIPGGWIADRITGTRGATLLG
                     AVFIIIGHICLSLPFALIGLFTSMFFIIIGSGLMKPNISNIVGRLYPENDRRMDAGFV
                     IFYMSVNMGALLSPIILQHFVNVKNFHGGFLIAAVGMALGLVWYVLFNRKNLGSVGMK
                     PTNPLTPAEKKKYGLIIGSVVLAIVLIIVIGALTNSLSFNLVSNTVLVLGIALPIIYF
                     TLIIRSKDVTDTERSRVKAFIPLFILGMVFWAIQEQGSNVLNIYGIEHSDMKLNLFGW
                     KTNFGEAIFQSINPLFILLLAPIISLLWQKLGTKQPSLPVKFAIGTFLAGASYILIGI
                     VGYASGSSNFSVNWVILSYIICVIGELCLSPTGNSAAVKLAPKAFNAQMMSIWYLTNA
                     SAQAINGTLVKLIEPLGQTNYFIFLGVVAIIVTTIVLAISPLIIKAMKGIR"
     gene            complement(3779..3880)
                     /locus_tag="O2701_001878"
     CDS             complement(3779..3880)
                     /locus_tag="O2701_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293764.1"
                     /translation="MVINEIDFYRVGRENIVIAVYNEHIKFLFILIS"
     gene            3971..4888
                     /locus_tag="O2701_001879"
     CDS             3971..4888
                     /locus_tag="O2701_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCZ4293765.1"
                     /translation="MENKYTHGVLFYHEHSGLKNINQGIGEVTTALSSICKHLSIQLS
                     ENEGDIIKYCQEIKTKNYAKDVDILFILGGDGTVNELINGVMTHDLQLPIGILPGGTF
                     NDFTKTLNIAPNHKQASEQMISAQVGTYDVIKINNQYALNFVGLGLIVQNAENVQDGS
                     KDIFGKLSYIGSTVKTLLNPTQFNYQLSIDDKTYSGETTMILTANGPFIGGSRIPLTD
                     LSPQDGELNTFIFNEQSFSILNDIFKKRDSMNWNEITQGIEHIPGKKISLTTDPAMKV
                     DIDGEISLETPIDIEVIPNAIQLLTVNDL"
     gene            complement(5512..6054)
                     /locus_tag="O2701_001880"
     CDS             complement(5512..6054)
                     /locus_tag="O2701_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'(3')-deoxyribonucleotidase"
                     /protein_id="HCZ4293766.1"
                     /translation="MTRKSIAIDMDEVLADTLGEIIDAVNFRADLGIKMEALNGQKLK
                     HVIPEHDGLITEVLRESGFFRHLKVMPYAQEVVKKLTEHYDVYIATAAMDVPTSFSDK
                     YEWLLEFFPFLDPQHFVFCGRKNIVKADYLIDDNPRQLEIFTGTPIMFTAVHNINDDR
                     FERVNSWKDVEQYFLDNIEK"
     gene            complement(6405..7463)
                     /gene="hisC"
                     /locus_tag="O2701_001881"
     CDS             complement(6405..7463)
                     /gene="hisC"
                     /locus_tag="O2701_001881"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCZ4293767.1"
                     /translation="MKEQLNQLSAYQPGLSPRALKEKYGIEGDLYKLASNENLYGPSP
                     KVKEAISAHLDELYYYPETGSPTLKAAISKHLNVDQSRILFGAGLDEVILMISRAVLT
                     PGDTIVTSEATFGQYYHNAIVESANVIQVPLKDGGFDLEGILKEVNEDTSLVWLCNPN
                     NPTGTYFNHESLDSFLSQVPPHVPVIIDEAYFEFVTAEDYPDTLALQQKYDNAFLLRT
                     FSKAYGLAGLRVGYVVASEHAIEKWNIIRPPFNVTRISEYAAVAALEDQQYLKEVTHK
                     NSVERERFYQLPQSEYFLPSQTNFIFVKTKRVNELYEALLNVGCITRPFPTGVRITIG
                     FKEQNDKMLEVLSNFKYE"
     gene            complement(7703..9217)
                     /locus_tag="O2701_001882"
     CDS             complement(7703..9217)
                     /locus_tag="O2701_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HCZ4293768.1"
                     /translation="MTNFFDILSERKGQLFSTMIEHIQISFIALLIATAIAVPLGILL
                     TKTKTISEIVMNIAAILQTIPSLALLGLMIPLFGIGRVPAIIALVVYALLPILRNTYT
                     GIKEVDPSLIEAAKGIGMKPFRRLTKVELPIAMPVIMAGVRTAMVLIIGTATLAALIG
                     AGGLGDLILLGIDRNNASLILLGAIPAALLAIIFDLILRFMAKLSYKKLLMTLGVIVM
                     IIILAIAIPMFAQKGDKITLAGKLGSEPSIITNMYKILIEEETKNTVEVKDGMGKTAF
                     LFNALKSDDIDGYLEFTGTVLGELTKEPLKSKEEKKVYEQAKQSLEKKYQMTMLKPMK
                     YNNTYALAVKRDFAKQHNIRTIGDLNKVKDQLKPGFTLEFNDRPDGYKAVQKAYNLNL
                     DNIRTMEPKLRYQAINKGNINLIDAYSTDAELKQYDMVVLKDDKHVFPPYQGAPLFKE
                     SFLKKHPEIKKPLNKLENKISDEDMQMMNYKVTVKNEDPYTVAKDYLKAKGLIK"
     gene            complement(9210..10187)
                     /locus_tag="O2701_001883"
     CDS             complement(9210..10187)
                     /locus_tag="O2701_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4293769.1"
                     /translation="MIKFKNVTKRYGKHVAVDNISFNINEGEFFVLIGPSGCGKTTTL
                     KMINRLIHLSEGYIYFKDKPISDYPVYEMRWDIGYVLQQIALFPHMTIKENIAQVPQM
                     KKWKEKDIDKRVDELLEMVGLEPEKYKNRKPDELSGGQRQRVGVIRALAADPPVILMD
                     EPFSALDPISREKLQDDLIELQTKIKKTIIFVTHDIQEAMKLGDKICLLNEGHIEQID
                     TPEGFKNNPQSEFVKQFMGSHLEDDAPCVEENAIIRDLDIMKPIDEVTSMSAYPIVYD
                     NQPIEVLYQLLSESERVIVMQEDSVGQYVIDRKDIFKYLSQKKEVAQHD"
     gene            complement(10409..12190)
                     /gene="recQ"
                     /locus_tag="O2701_001884"
     CDS             complement(10409..12190)
                     /gene="recQ"
                     /locus_tag="O2701_001884"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HCZ4293770.1"
                     /translation="MMQQTLSHYFGYETFRPGQEEIISKVLDHRNVLGVLPTGGGKSI
                     CYQVPGLLLGGTTIVISPLISLMKDQVDQLKAMGIQAAFLNSSLTQKEQQRIEKALSN
                     GEIQFLYVAPERFENRYFLNMLQRIKIHLVAFDEAHCISKWGHDFRPSYQNVISKVFT
                     LPQDFTIIALTATATVEVQQDIREKLNIAQTDQIKTSTKRRNLIFKVNPTYQRQKFIL
                     DYIKTHDEDAGIIYCSTRKQVEELQEALESQKIESVIYHAGLSNKEREEAQNDFLFDR
                     VKVVVATNAFGMGIDKSNVRFVIHYNMPGDLESYYQEAGRAGRDGLKSECILLFSERD
                     INLHEYFITVSQADDDYKDKMGEKLTKMIQYTKTKKCLEATIVHYFEPNEKLEECEQC
                     SNCVQQDKSYNMTQEAKMIISCIARMKQQESYSVIIQVLRGESTDYIKYKGYDQISTH
                     GLMKGYTTSELSHLIDELRFKGFLNENDEILMCDTSIKKLLSNEVEVFTTPFKQKATE
                     KVFINTVEGVDRVLFSQLVEVRKKLSDKLTIAPVSIFSDYTLEEFAKRKPASKQDMIN
                     IDGVGSYKLKHYCPAFLETIQNYKAKV"
     gene            complement(12202..14085)
                     /locus_tag="O2701_001885"
     CDS             complement(12202..14085)
                     /locus_tag="O2701_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCZ4293771.1"
                     /translation="MGMEAYKIEHLNKSYADKTIFDNLDLSISEGEKIGLVGINGTGK
                     STLLKVIGGIDDDFTANVMHPNQYRIRYSSQKQDLNEDMTVFDAVLSSDTTTLRIIKQ
                     YEQAVQAYADDQSDKLFKRMMDAQDAMDQHDAWDYNAEIKTILSKLGIHDTTKYIKEL
                     SGGQQKRVVLAKTLIEQPDLLLLDEPTNHLDFESISWLINYVKQYPHTVLFVTHDRYF
                     LNEVSTRIIELNRGKLASYPGNYESYIEMRAEREVTLQKQQQKQRALYKEELAWMRAG
                     AKARTTKQQARINRFNDLENEVNQQYKDDKGELNLAYSRLGKQVFELEDLSKAINDKV
                     LIEHLTEIIQKGERIGVVGPNGAGKTTLLNILSGEDQQFEGKLKTGQTVKVAYFKQTD
                     ETLDRDIRMIDYLREENEIAKEKDGTSVSITQLLERFLFPSATHGKKVYKLSGGEQKR
                     LYLLRLLVHQPNVLLLDEPTNDLDTETLTILEDYIHTFGGTVITVSHDRYFLNKVAQS
                     YWFIHDGQMEKIIGTFEDYESYKKSLDKNKSTLKQQSKSSTTVRKKNGLSYKEKLEYE
                     QLMKRIEQAEVRMEEIDVLMIEASADYGKIKELNEEKEQLEIQYDLDITRWSELEEIK
                     EQQ"
     gene            complement(14356..16296)
                     /gene="ltaS"
                     /locus_tag="O2701_001886"
     CDS             complement(14356..16296)
                     /gene="ltaS"
                     /locus_tag="O2701_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyglycerol-phosphate lipoteichoic acid
                     synthase LtaS"
                     /protein_id="HCZ4293772.1"
                     /translation="MSSQKKKISLFAFFLLTVITITLKTYFSYYVDFSLGVKGLVQNL
                     ILLMNPYSLVALVLSVFLFFKGKKAFWFMFIGGFLLTFLLYANVVYFRFFSDFLTFST
                     LNQVGNVESMGGAVSASFKWYDFVYFIDTLVYLFILIFKTKWLDTKAFSKKFVPVVMA
                     ASVALFFLNLAFAETDRPELLTRTFDHKYLVKYLGPYNFTVYDGVKTIENNQQKALAS
                     EDDLTKVLNYTKQRQTEPNPEYYGVAKKKNIIKIHLESFQTFLINKKVNGKEVTPFLN
                     KLSSGKEQFTYFPNFFHQTGQGKTSDSEFTMDNSLYGLPQGSAFSLKGDNTYQSLPAI
                     LDQKQGYKSDVMHGDYKTFWNRDQVYKHFGIDKFYDATYYDMSDKNVVNLGLKDKIFF
                     KDSANYQAKMKSPFYSHLITLTNHYPFTLDEKDATIEKSNTGDATVDGYIQTARYLDE
                     ALEEYINDLKKKGLYDNSVIMIYGDHYGISENHNNAMEKLLGEKITPAKFTDLNRTGF
                     WIKIPGKSGGINNEYAGQVDVMPTILHLAGIDTKNYLMFGTDLFSKGHNQVVPFRNGD
                     FITKDYKYVNGKIYSNKNNELITTQPADFEKNKKQVEKDLEMSDNVLNGDLFRFYKNP
                     DFKKVNPSKYKYETGPKANSKK"
BASE COUNT         5226 a         2880 c         2375 g         6029 t
ORIGIN      
        1 gcatttgcat acaaaacaca agatattgtg tctttttcgt ttcaccaaca ctatattatg
       61 tgtttcatta tacataagac acactatgat ttaaagtgcc attttctaat cgaatgttcg
      121 taaaacgttc caactaggca ccaatactaa attagaaaaa cttgaacaaa tttaaaaaaa
      181 ttttttagta gatatcactt gattttttta aatctgtgtc aatctcaatt gtatcaacag
      241 aaatcaagaa aaagcttatt agcttgatac tgaattgata tttgttttat gtaataacac
      301 aaacattcat aaaagtgtaa tgccctatta ttccgacaaa taaacacaga acgtttgttc
      361 gctacagtct attatagtct ttttatagta aatttcaact atttatgcac aacatgttgt
      421 tttatgctaa tataaatacg ttaattttat ataaaagagg cgataaaatt gaatcctaaa
      481 gtaaaaggta tcattgccat tttaatttcg gctattggtt tcagttttat gtcagtgttt
      541 ttcagacttg caggtgacct tcctgttttt caaaaatcat tagcacgaaa cttagtagca
      601 atgtttattc cattattttt tatatataaa taccatcagc caatgtttgg cagactttca
      661 agtcaaccct tattaataac acgatcaaca cttggactta ttggtgtatt gttaaatatt
      721 tatgccattg atcatatggt acttagtgat gccgattcat taatgaaact caatcctttc
      781 tggacgattt tattaagcat tgttttctta catgaaaagg taaggaagta tcaaattaca
      841 gcaatgataa ttgctatttt aggtatgctt ttaatagtga aacctgaatt ttcatcatct
      901 atgattccat cattagctgg cttattctca ggtatttttg cagcatcagc atatacatgt
      961 gttcgagcac ttagtacaag agaagcacca tatactatag tattttactt ctccttattt
     1021 tctgtcatcg ttttaatacc tttcactgca tatacttatg aaccaatgag tcagatgcaa
     1081 atactttatc tattaggggc tggcttagct gccgctgtgg gtcaaattgg tgttacttta
     1141 gcatatagtt ttgcagcagc aaaagatatt tctattttta cgtatgcatc tataattttc
     1201 acagcaatac ttggatttat tctattcgga gaatctccag atttctatgc aacattaggt
     1261 tacgtcgtta ttatcggtgc aagttattac atgtttgaaa aagcacgccg agacgccaaa
     1321 ataataaaaa aataaattta aaggagcgct ttatatggca catggccgtc aacaagatga
     1381 attacaagat ataactttat taggaaatca agacaatact tacaattttg actatcgtcc
     1441 tgatgtatta gaatcgtttg ataataagca tcaaggtcgt gactatttcg taaaattcaa
     1501 ctgtccagaa tttacatcat tatgtccaat tactgggcaa ccagattttg caactatcta
     1561 tatttcatat attccaaatg ttaaaatggt tgaatcaaaa tctttgaaat tatacttatt
     1621 tagtttcaga aatcacggtg attttcacga agattgtatg aatattatta tgaatgattt
     1681 gatagagctt atggacccac attatattga agtctggggc aagttcacac cacgtggtgg
     1741 aatttctatt gatccttata caaactatgg acgtccaaat tctaaatatg aaaaaatggc
     1801 tgagcatcgt ttgatgaatc atgatttata tcccgaaaaa atagataatc gttaaatgta
     1861 tcatttaata aacacaccaa taagttgatt ttcctaactt attggtgtgt ttttcattta
     1921 gcatacataa taggttacat taaaataaca ttttatacca aagtacacca aaagaatatt
     1981 agtacacgaa ttaaacaaca tttttataga aacctattgc actttaacgt caataagtat
     2041 atttttatat tatctctaat taattgtgcg cgcttaataa cagaatattc tcaatatttt
     2101 tatttttttg tgatttgttg gaatatttag ttgataaggc acaatcaaat ttacttaaac
     2161 tattgtatta ggggaagaaa ggatgggatg tatacatgac acaacaaaac tcccatggaa
     2221 atcaaattca agacatacct caaacaggat ttttcgggca tcctcgagga ctaggcgtac
     2281 tcttctttgt agagttctgg gaaaggttta gttattatgg catgcgtgcc ctactcattt
     2341 tctacatgta ctttgccgta acagataatg gccttggaat tgataaaaca acagctatgt
     2401 caattatgtc agtttatggt tcattaatct atatgacatc aataccaggc ggatggattg
     2461 ctgacagaat tacaggcact agaggcgcta ctttattagg tgcagtcttt attattatcg
     2521 gacatatttg tttaagctta ccatttgcat taatcggctt attcacatca atgttcttca
     2581 tcattattgg ttcaggttta atgaagccaa acatttcaaa tatcgttggc cgtttatatc
     2641 ctgaaaatga tagacgtatg gatgcaggtt ttgttatttt ctatatgtca gttaatatgg
     2701 gtgcattatt atcacctatt attttgcaac actttgttaa tgttaaaaac ttccacggcg
     2761 gattcttgat tgcagcagtt ggtatggcat taggtttagt atggtatgta cttttcaacc
     2821 gcaaaaactt aggtagcgtt ggtatgaaac ctactaaccc attgacacca gctgaaaaga
     2881 aaaagtatgg tcttattatc ggaagtgttg ttttagcaat tgtattaatt atcgttattg
     2941 gtgcattaac taactcatta tcatttaact tagttagtaa tactgtttta gtattaggta
     3001 ttgcattacc aatcatttac ttcactttaa ttattagaag taaagatgtc actgatactg
     3061 aacgttctcg tgttaaagca tttattccat tatttattct tggaatggtg ttctgggcta
     3121 ttcaagaaca agggtctaat gtattaaaca tatatggaat tgaacattca gatatgaaat
     3181 taaacttatt tggttggaaa acaaactttg gtgaagcgat cttccaatca attaacccat
     3241 tatttatttt attactagct ccaattattt cacttttatg gcaaaagctt ggaactaaac
     3301 aacctagcct gccagtaaaa tttgcaattg gtacgttctt agcaggtgcg tcatacatac
     3361 taattggtat tgtaggttat gcatcaggtt catcaaactt ttcagttaac tgggttattt
     3421 tatcgtacat tatttgtgtt atcggtgagc tatgcttatc accaacgggt aatagtgctg
     3481 ctgttaagtt agcacctaaa gcatttaatg cccaaatgat gagtatttgg tacttaacta
     3541 acgcttctgc acaagcaatc aatggtactt tagttaagtt aatagaacca ctaggtcaaa
     3601 caaattactt tattttccta ggtgttgttg caattattgt tacaacaatt gtattagcaa
     3661 tctcaccttt aatcatcaaa gcgatgaaag gtatacgtta atattgttgg cctaattcaa
     3721 aaaacagtaa gtcatttaaa tggcttactg tttttttata ggtttctatt aattaaattc
     3781 aagatatcag tataaataaa aacttaatat gctcattata gacagctata actatatttt
     3841 ctcgtcccac tctatagaaa tcaatttcat ttattaccat tttctctatt taaaaacata
     3901 tttttggtat taacaattaa aagtgggtat atatatttaa tgaaccaatt tttagaaagt
     3961 aggctaaact atggaaaata aatatacaca tggtgtcctc ttttaccatg aacacagtgg
     4021 attaaaaaat attaatcaag gtattgggga agttacaaca gcattaagtt caatttgtaa
     4081 gcatctttct attcaattga gtgaaaacga aggtgacatt attaaatatt gccaagaaat
     4141 caaaactaaa aactatgcaa aagatgtaga tatattattt attcttggcg gtgatggcac
     4201 ggttaacgaa cttataaacg gtgttatgac acatgacctt caactcccta ttggcatttt
     4261 accaggcggt acttttaatg attttacaaa aacgctaaat atagcaccta atcataaaca
     4321 agctagtgaa caaatgattt ctgcacaagt tggtacatac gatgtaatta aaataaataa
     4381 tcaatatgca ctcaactttg ttggcttagg tctcattgtt caaaacgctg aaaacgtaca
     4441 agatggttca aaagatatat ttggtaaatt gagttatatt ggatcaactg tcaaaacact
     4501 gctaaatcca acacaattta attatcaatt atcaatagat gataagacat attctggcga
     4561 aacgaccatg atactaacag caaatggtcc attcattggt ggtagtcgta taccattgac
     4621 tgatttgtct cctcaagatg gtgaactgaa cactttcatt tttaatgaac aaagtttcag
     4681 tattttaaat gatattttta aaaagcgtga tagcatgaat tggaatgaaa ttactcaagg
     4741 tattgagcat atacctggta aaaagatttc acttacaaca gatccagcaa tgaaagttga
     4801 tattgatggc gaaatttcat tagaaacacc tattgatatt gaggttatac caaatgccat
     4861 tcagttactt actgtcaatg atttgtaagc atccggaatg atcttaaccc gatattatat
     4921 taggtttgga gtgtaatcat aagattcaac gcaccttata ttcgttttta ttagctttaa
     4981 gctattaccc tgtttcaaaa acagacatat aattttggcg catgccacta aaatgattga
     5041 tgttgtattt taaatcatag tggtttatgc gtctttttca aattctataa aaaatcggat
     5101 gacgtgtaat ctgccataga ttaacacatt catccgattt ataataataa gataaactaa
     5161 catttattga gagcgggacg gaaatgataa agaacgacta atgattgatt atgtagcgat
     5221 tctttatcat tagtcacagc taatgtgtac ttaaaaatat gaatgcatga gttacactca
     5281 tgcataagaa atactaattt ctaaagaaaa agtatttctt tatgttgggg cccaccccaa
     5341 cttgcattgt ctgtagaaat tggcgttcca atttctctat gttggggccc cgcagtcaac
     5401 tactgccaat atagtgttgt agtgtctaag acataaaagt tcatctcagt cacaattact
     5461 ttatagccta tccatcatct tttctacatg aaatttttca agtgatatat tttatttctc
     5521 aatattatct aaaaaatact gttctacatc tttccagcta tttacgcgtt caaatcgatc
     5581 atcattaata ttatgcactg ctgtaaacat aatcggtgta ccagtaaaaa tttcaagctg
     5641 tctaggattg tcatctatta aataatcagc tttaacgatg ttttttctac cacaaaaaac
     5701 aaaatgctga ggatctaaaa atggaaagaa ctctagtaac cattcatatt tatcactaaa
     5761 tgatgttggt acatccattg ctgctgtagc aatatataca tcataatgtt cagttaattt
     5821 tttcacaact tcttgtgcat acggcatcac tttaagatgt ctgaagaagc ctgattctct
     5881 caatacttct gtaattaatc catcatgttc aggaataaca tgtttaagtt tttgaccatt
     5941 caaagcttcc attttaatac ctaaatccgc tctaaaattg acagcatcaa tgatttctcc
     6001 taatgtatct gccaatactt catccatatc aatcgcgatt gatttacggg tcattttaat
     6061 cttctcctca aaatatcagt attgattcag tatagcaaaa aagcaattga tacaaaacaa
     6121 tatcatctat tgttgctttt tacactgaat actctcacct catcattcac actgcattga
     6181 gcttgagaca taaatcaatg ttcaatgctc tacaaagtta tattggcagt agttgactga
     6241 acgaaaatgc gcttgtaaca agcttttttc aattctagtc aaccttgccg gcggggcccc
     6301 aacaaagaga aattggattc ccaatttcta cagacaatgc aagttggggt gggacgacga
     6361 aataaatttt gcgaaagtat catttctgtc ccactcccca cttactattc gtatttaaag
     6421 tttgataaaa cttctaacat tttatcattt tgttctttaa aaccaattgt aattctaaca
     6481 ccagttggaa atggtcgcgt aatacaccct acatttaaaa gtgcttcata aagttcattt
     6541 acccgctttg tttttacaaa tataaaattc gtttgacttg gcaagaaata ctcactttga
     6601 ggtaattgat aaaatctttc gcgttcaaca ctatttttat gtgttacctc ttttaaatat
     6661 tgttgatctt caagtgctgc aactgctgcg tattcagata tacgtgtcac attaaatggt
     6721 ggtctaatga tgttccattt ttcaatcgca tgttcacttg ctaccacata tcctacacgt
     6781 aaacccgcta atccatacgc ctttgaaaat gtacgtaata agaaagcatt gtcatatttt
     6841 tgttgcaaag caagtgtatc cgggtagtcc tctgctgtca caaattcaaa ataagcttca
     6901 tctataatta ctggtacatg tggaggtact tgagataaaa acgaatctaa gctctcatga
     6961 ttaaaatatg tacctgtagg attatttgga ttacataacc ataccaatga cgtatcttca
     7021 ttaacttctt ttaaaatacc ttctaaatcg aagccaccat cttttaaagg tacttgtatc
     7081 acattagctg attcaacaat cgcattgtga taatattgac cgaatgtcgc ttcacttgta
     7141 acaatagtat cccctggcgt taatacagct ctagaaatca ttaatataac ttcatctaat
     7201 cccgcaccaa ataaaatgcg tgattgatct acatttaaat gtttactaat cgccgctttt
     7261 aatgtcggtg atcctgtttc aggataataa tataactcat ctaagtgtgc tgatatcgct
     7321 tctttaactt taggcgatgg tccatacaaa ttttcatttg atgcaagttt atataaatct
     7381 ccttcaatgc catacttttc tttcaatgcc cttggagata aaccaggctg atatgctgat
     7441 agttgattaa gttgttcttt cataataaaa acaacctcct tagaattaac tgaaatttca
     7501 aaaaacttat attcattata tcgctctatg tttctaaaat gcaatgctac tattttcaca
     7561 tcataatagc ttacattgtg tttaacatgt catatttttt aatcattatt gcatgtttaa
     7621 attgaacata attatagatt taaatataac aacgccacat aagatataat ttggtgttac
     7681 gcatcttatg tggcgttggt cgttatttga ttaacccttt tgcttttaaa taatctttcg
     7741 caactgtata tgggtcttca tttttaactg ttactttata gttcatcatt tgcatatctt
     7801 catcagatat tttgttttct agtttgttta acggtttctt aatttctgga tgtttcttta
     7861 aaaagctttc tttaaataat ggtgctcctt gatatggtgg aaatacgtgc ttatcatctt
     7921 ttaacacaac catatcatat tgttttaatt cagcgtcagt tgaatatgca tctattaaat
     7981 taatattacc tttattgatc gcttgatatc tcaacttagg ttccattgta cgtatgttat
     8041 ctaaatttaa attataagcc ttttgaacag ctttgtaacc atctggacga tcattaaatt
     8101 ccaatgtaaa tcctggttta agttgatctt taaccttatt taaatcacca attgtacgta
     8161 tattatgttg tttagcaaaa tcacgtttta cagctaaagc atacgtattg ttatacttca
     8221 ttggttttaa catagtcatt tgatatttct tttcaagact ttgcttagct tgttcataaa
     8281 cttttttctc ttcttttgac ttcaatggtt cttttgttaa ttcacctaaa actgttccag
     8341 taaattctaa atacccatct atatcgtcag attttaaagc attaaataaa aatgctgttt
     8401 tgcccatacc atctttcact tctacagtat ttttggtctc ttcttctatt aaaattttat
     8461 acatatttgt aataatcgat ggctcggagc caagctttcc agctaacgta attttatcac
     8521 ctttttgtgc aaacatagga atagcgatag ccagtataat aatcatcact ataacaccta
     8581 acgtcatcaa taacttttta taagataatt tagccataaa tcttaaaatt aaatcaaata
     8641 taattgccaa taaggctgct ggaattgcac ctaataatat caacgatgca ttgttacggt
     8701 ctatacctaa taaaattaaa tctcctagtc cgcctgcacc aattaatgct gctagtgttg
     8761 ctgtacctat aattaatacc atagccgttc ttacaccagc cattataaca ggcattgcta
     8821 tcggaagttc gactttagtt aaacgtctaa atggtttcat acctatacct ttagccgctt
     8881 caatgagtga tggatcaact tctttaattc cagtatacgt attccttaaa attggtaaca
     8941 acgcatacac tacaagtgca ataattgctg gcacacgacc gataccaaat aaaggaatca
     9001 ttaaacctaa taatgccaac gatggtatgg tttgaagaat tgccgcaata ttcattacga
     9061 tttcagatat cgttttagtc ttcgttaata aaatacctaa tggtaccgca atagcagttg
     9121 caatcaataa tgcgataaat gatatttgaa tatgttctat cattgtcgaa aagagttgcc
     9181 ccttacgttc actcaatatg tcgaaaaagt tagtcatgtt gagctacctc ctttttctgg
     9241 gacaaatatt tgaagatatc tttcctatca ataacatatt gacctacgct atcttcttgc
     9301 atgacaatga cacgctcgct ctctgataaa agttgataca atacttcaat tggttgattg
     9361 tcataaacaa ttggataagc gctcatagat gtaacctcat cgattggttt cataatatcc
     9421 aagtcacgga taattgcgtt ctcttcaaca catggcgcat catcttctaa atgactaccc
     9481 ataaattgtt taacaaattc actttgagga ttatttttaa atccttctgg tgtgtcaatt
     9541 tgttcaatat gcccttcatt caaaagacaa atcttatcac caagtttcat cgcctcttga
     9601 atatcatgtg taacaaatat gattgtcttc ttaattttag tttgtaattc aattaaatca
     9661 tcttgaagtt tttctcggct gattgggtct aatgcactaa acggttcatc cattaaaata
     9721 actggtggat cagctgctaa cgcacgtata actcctacac gttgtcgttg cccccctgac
     9781 aattcatcag gttttctgtt tttatatttt tcaggttcta atccaaccat ttcaagtaat
     9841 tcatctactc ttttatctat atctttttct ttccactttt tcatttgtgg cacttgtgca
     9901 atattttctt tgattgtcat atgtgggaat aatgcaatct gctgcaatac gtatccaata
     9961 tcccaacgca tttcgtatac tggataatca cttattggtt tatctttaaa ataaatataa
    10021 ccttcactta agtgaatgag tcgattaatc atttttaatg tcgtagtttt tccacaacct
    10081 gaaggtccaa ttagcacaaa aaattcaccc tcattaatat tgaaactaat gttatcgaca
    10141 gcaacatgtt tgccataacg cttagttaca tttttaaact taatcacttt gccacctctt
    10201 tttttctcat agcataaaac cgagattata tgtatgtatt ccctatttaa ccacgtttat
    10261 tacaattttc aaatttaaat gatttatcct tgaacttttt taataaaata atgaataata
    10321 ggtaatctcc agttaagaaa tagtgttatt ttaccttgaa ttcaaaaaaa cacccagtaa
    10381 aacaaggaat gcttactagg tgtcttcact atactttggc tttataattt tgaatcgttt
    10441 ctaaaaatgc tggacaataa tgttttaatt tgtaactacc tacgccatca atattaatca
    10501 tatcttgttt cgaagcaggc ttacgtttag caaattcctc caacgtgtaa tcagaaaata
    10561 tacttacagg tgctatcgtt aatttgtcac ttaacttttt acgaacttct accaactgac
    10621 tgaataatac tcggtcaacc ccttcaaccg tatttataaa tactttttca gtcgcttttt
    10681 gcttaaatgg tgttgtgaat acttctactt cattactgag taatttttta attgaagtat
    10741 cacacattaa tatttcgtca ttttcattta agaacccttt gaatcttaat tcatctatta
    10801 agtgacttaa ttctgatgtt gtgtaacctt tcattaaacc atgggttgaa atttggtcat
    10861 aacctttata cttaatataa tctgttgact ctcctcttaa cacttgaatg ataacactat
    10921 aactctcttg ttgtttcata cgagcgatgc aactaataat catcttagct tcttgtgtca
    10981 tattatatga tttatcttgt tgaacacaat tactacattg ttcacattct tctaattttt
    11041 cattcggttc aaaataatgg acaattgttg cttctagaca tttttttgtt tttgtatatt
    11101 gaatcatttt agttaacttt tcgcccattt tatctttata gtcatcatca gcttgagaga
    11161 ctgttataaa atactcgtgt aaattgatat cgcgttcgct aaataacaaa atacattcac
    11221 ttttcaaccc gtcacgacct gcacgacccg cttcttgata ataagattct aaatctccag
    11281 gcatattata atgaataaca aagcgtacat tggatttatc aatacccata ccaaaagcat
    11341 ttgtagcaac gactacttta acacgatcaa ataagaaatc attctgcgct tcttctcttt
    11401 ctttattgct caaacctgca tgatatataa cactttcaat tttctgactt tctaaggctt
    11461 cttgaagctc ttcaacttgc ttacgtgtag aacaataaat aatacctgca tcttcatcgt
    11521 gtgttttaat ataatccaat ataaattttt gacgttgata agtaggattt actttaaaaa
    11581 ttaagtttct acgcttagta ctcgttttaa tttgatcagt ttgagcgata tttaactttt
    11641 ctctaatatc ttgctgtact tcaaccgtgg cagttgctgt caacgctatt attgtaaaat
    11701 cttgaggtaa cgtaaatact tttgaaataa cattttggta actcggcctg aaatcatgac
    11761 cccatttaga aatacaatgc gcttcatcaa acgcgactaa gtgaatcttt atacgctgaa
    11821 gcatatttaa aaaatatcgg ttttcaaatc gttctggtgc aacatacaaa aattgaattt
    11881 ctccatttga taatgctttt tcaatacgtt gttgctcttt ttgagtcaaa ctactattta
    11941 aaaaagcagc ttgaattccc atcgctttta attgatccac ttgatctttc attaatgata
    12001 ttagtggact tattacaatt gttgtaccac ctaacaataa acctggtact tgatagcata
    12061 tagacttacc tccaccagtt ggtaagacac caagcacatt acgatggtct aatactttgc
    12121 taataatttc ttcttgtcct ggtcgaaacg tttcataccc aaagtaatgc gataatgttt
    12181 gttgcatcat aaattgaccc cttattgttg ttctttaatt tcttctaact cactccatct
    12241 tgtgatgtct aaatcatatt gaatttcaag ttgttctttt tcttcgttta attctttaat
    12301 tttcccataa tctgcacttg cctcaatcat gagcacatca atttcttcca ttcttacttc
    12361 cgcttgttct atgcgtttca tcaattgttc atattctaat ttttctttat atgataaacc
    12421 atttttctta cgtacagttg tagaagattt agattgttgc ttcaatgtgg atttattttt
    12481 atctaatgat tttttataac tttcataatc ttcaaaagtt ccgataatct tttccatctg
    12541 accatcatga ataaaccaat atgactgtgc aactttattt aagaagtagc gatcatggct
    12601 tacggtaatc actgtaccac cgaaagtatg aatataatct tcaagtattg ttaaagtctc
    12661 agtatctaaa tcatttgtcg gttcatctaa caacagaaca tttggctggt gtacgagtag
    12721 acgtaataaa tacaaacgct tttgctctcc accagataat ttataaactt ttttaccatg
    12781 agttgcactt ggaaataaaa atcgttcaag aagttgtgta atagataccg aggttccatc
    12841 tttttctttt gcgatctcat tttcttctct taaataatca atcatacgaa tatctctatc
    12901 cagggtctca tctgtttgct taaaataagc tactttaacc gtctgcccag tcttcaattt
    12961 accttcgaat tgttggtctt ctccactcaa aatatttaag agtgttgttt taccagctcc
    13021 atttggccca acaacaccaa tacgctcacc tttttgaata atttccgtca gatgttcaat
    13081 taatacttta tcattaatag cctttgataa gtcttctaat tcgaacactt gcttacctaa
    13141 tcttgaataa gcaagattca attcaccttt atcgtcttta tattgctggt taacttcatt
    13201 ttctaggtca ttaaatcgat taattctagc ttgttgcttt gtagtacgag ccttagctcc
    13261 agccctcatc caagcaagtt cttccttata taaagctcgt tgcttttgtt gttgcttttg
    13321 aagtgttact tctctttcag cgcgcatttc aatataagat tcatagttac caggatatga
    13381 cgctaactta cctctgttta gttcaataat tctagtggaa acttcattta aaaaatatcg
    13441 atcatgggtt acgaataaaa cagtatgagg atattgcttc acataattga tcaaccagct
    13501 gattgattcg aagtctaaat ggttcgtagg ttcatctaat aacaataaat ctggttgttc
    13561 tattaatgtt ttagcaagta caacacgttt ttgttgtccg ccggataatt ctttaatgta
    13621 tttagtagta tcatgtatac ctagttttga gaggattgtt ttaatttcag cgttatagtc
    13681 ccaagcatca tgttgatcca tagcatcttg cgcatccatc attcgcttga acaatttatc
    13741 actttggtca tccgcataag cttgtactgc ctgctcatat tgcttgatga tgcgtaaagt
    13801 tgttgtatca gaacttaata ctgcatcaaa aactgtcata tcttcattaa ggtcctgttt
    13861 ctgagacgaa tatcgaattc gatattgatt tggatgcata acattggctg taaaatcatc
    13921 atcaatacca ccaattactt ttaacaacgt acttttccct gtaccattta tgcctactaa
    13981 acctattttt tcaccttctg aaattgataa atctaggtta tcgaatatag tcttatcggc
    14041 ataagattta tttaaatgtt caattttata tgcttccata cccatcttcc ttcttctgtt
    14101 gaaatctcta cgtacttaaa tcagttctat acattataca cctttttgaa atcaaaagaa
    14161 atcgataccg ttaagtgtca taatttaata tattgttgct tcaactataa ataaaacttg
    14221 ttaaggcttt gatgactatg ctaatgatct ttataatata cctttttatc aacacatata
    14281 atgtaaaaca taatataaaa gaacataata aaaacccgga ttgatatttt atcaatccga
    14341 gttcgtgttt agatattatt ttttagagtt tgctttaggt cctgtttcat atttatactt
    14401 cgaaggattt acctttttga agtctggatt tttgtagaat ctaaacaaat caccattaag
    14461 cacgttgtca ctcatttcga gatccttttc aacttgcttt ttattctttt cgaaatcagc
    14521 tggttgagta gttatgagtt cattattttt attagaataa atcttaccat taacatattt
    14581 ataatctttt gttataaagt caccatttct gaatggaact acttgattat gacctttaga
    14641 gaataaatca gtaccgaaca ttaaatagtt cttcgtatct ataccagcca aatgtaaaat
    14701 tgttggcatt acatcgactt gaccagcata ttcattattg ataccaccag atttaccagg
    14761 gattttaatc cagaaaccag ttctgtttaa atctgtaaat ttagccggtg tgattttttc
    14821 acctaatagt ttttccatgg cattgttatg gttttcagag ataccatagt ggtcaccata
    14881 aatcataatc actgaattgt catataatcc ttttttcttc aagtcattaa tatattcttc
    14941 taatgcttcg tctaaataac gtgctgtttg aatataacca tcaactgttg catcacctgt
    15001 gtttgatttc tcaatagttg catccttttc atctaatgtg aatggatagt ggttagtcaa
    15061 tgtaattaaa tgagaataga atggtgattt catcttagct tgataattag cagaatcttt
    15121 aaagaaaatt ttgtctttca agcctaagtt tacaacgttt ttatctgaca tgtcatagta
    15181 tgttgcatca tagaatttat cgataccaaa gtgtttatat acttggtctc tgttccagaa
    15241 tgttttatag tcaccgtgca tgacatcaga tttgtagcct tgcttttgat ctaaaattgc
    15301 tggtaatgac tgatacgtat tatctccttt taatgaaaag gcagaacctt gcggtaaacc
    15361 gtataaactg ttatccattg taaattcaga gtcagatgtt ttaccttgac ctgtttgatg
    15421 gaaaaagtta gggaagtatg tgaattgctc tttcccactt gataatttgt ttaaaaacgg
    15481 tgttacttct ttaccattaa cctttttatt aattaagaag gtttggaaac tttctaaatg
    15541 aatcttaata atatttttct tctttgccac cccataatat tctgggttag gctctgtttg
    15601 acgttgtttc gtataattta atacttttgt taagtcatct tcagatgcta gcgctttttg
    15661 ttgattattt tcgatagttt taacaccatc gtatactgta aagttataag gtcctaaata
    15721 tttcactaaa tatttatggt caaatgtacg tgttaataat tctggtctgt cagtttcagc
    15781 aaaagctaag tttaagaaga ataatgctac tgaagccgcc attacgacag gaacaaattt
    15841 cttactaaat gcttttgtgt ctaaccattt tgttttaaat attaaaatga ataagtaaac
    15901 taacgtatca atgaaataaa caaagtcata ccatttgaat gatgcactaa ccgcaccacc
    15961 catagattct acgttaccta cttggtttaa agtactaaac gttaaaaaat cagagaagaa
    16021 tctaaagtac acaacattgg catataatag gaacgtcaat aagaagccgc ctatgaacat
    16081 gaaccaaaat gcttttttgc ctttaaagaa taggaacaca cttaaaacca gtgctactaa
    16141 actataagga ttcatcaata atattaagtt ttgtactaaa cctttaacac ctaaagaaaa
    16201 atcaacataa taagaaaaat acgtcttcaa ggtaatcgtt attacggtta ataagaagaa
    16261 cgcaaaaaga ctaattttct ttttttgtga actcatgatt ctttcccccg ttatttagat
    16321 aataaatctt gcgaaagtac ttgaataaat tagatattaa cattatgtag ttaattaata
    16381 agtgcgttta aaagttaaca ttgtcataat tatagtaatt actttcagat tcaagtttat
    16441 aattttcgtt atttgtagct ttcaactttg atttaacaag tattttgggc tacaactcat
    16501 atttgcttgt
//
LOCUS       DALAGD010000071        15979 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.072, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000071 DALAGD010000000
VERSION     DALAGD010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15979
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.072"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            15..1181
                     /gene="scpA"
                     /locus_tag="O2701_001887"
     CDS             15..1181
                     /gene="scpA"
                     /locus_tag="O2701_001887"
                     /EC_number="3.4.22.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain A"
                     /protein_id="HCZ4293773.1"
                     /translation="MKRNFPKLIALSLIFSLSVTPIANAESNSNIKAKDKKHVQVNVE
                     DKSVPTDVRNLAQKDYLSYVTSLDKIYNKEKASYTLGEPFKIYKFNKKSDGNYYFPVL
                     NTEGNIDYIVTISPKITKYSSSSSKYTINVSPFLSKVLNQYKDQQITILTNSKGYYVV
                     TQNHKAKLVLKTPRLEDKKLKKTESIPTGNNVTQLKQKASVTMPTSQFKSNNYTYNEQ
                     YINKLENFKIRETQGNNGWCAGYTMSELLNATYNTNKYHAEAVMRFLHPNLQGQRFQF
                     TGLTPREMIYFGQTQGRSPQLLNRMTTYNEVDNLTKNNKGIAVLGSRVESRNGMHAGH
                     AMAVVGNAKLDNGQEVIIIWNPWDNGFMTQDAKNNVIPVSNGDHYRWYSSIYGY"
     gene            1212..1538
                     /locus_tag="O2701_001888"
     CDS             1212..1538
                     /locus_tag="O2701_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin A"
                     /protein_id="HCZ4293774.1"
                     /translation="MEQIELFSIDKFKCNSEAKYYLNIIEGEWHPQDLNDSPLKFILS
                     TSDDSDYICKYINTEHKQLTLYNKNNSSIVIEIFIPNDNKILLTIMNTEALGTSPRMT
                     FIKHKS"
     gene            complement(1830..2003)
                     /locus_tag="O2701_001889"
     CDS             complement(1830..2003)
                     /locus_tag="O2701_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NETI motif-containing protein"
                     /protein_id="HCZ4293775.1"
                     /translation="MKFKVNENETIADCLSRMKMQGYMPVKRIEKPVFQEQKDGTVEV
                     SHQEIVFVGKKIQ"
     gene            complement(1984..2586)
                     /locus_tag="O2701_001890"
     CDS             complement(1984..2586)
                     /locus_tag="O2701_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HCZ4293776.1"
                     /translation="MSFVTENPWLMVLTIFIINICYVTFLTMRTILTLKGYRYIAASV
                     SFLEVLVYIVGLGLVMSNLDHIQNIIAYAFGFSIGIIVGMKIEEKLALGYTVVNVTSA
                     EYELDLPNELRNLGYGVTHYAAFGRDGSRMVMQILTPRKYERKLMDTIKNLDPKAFII
                     AYEPRNIHGGFWTKGIRRRKLKDYEPEELESVVEHEIQSK"
     gene            complement(2857..3678)
                     /gene="nadE"
                     /locus_tag="O2701_001891"
     CDS             complement(2857..3678)
                     /gene="nadE"
                     /locus_tag="O2701_001891"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCZ4293777.1"
                     /translation="MSKLQDVIVQEMKVKKRIDSAEEIMELKQFIKNYVQSHSFIKSL
                     VLGISGGQDSTLVGKLVQMSVNELREEGIDCTFIAVKLPYGVQKDADEVEQALRFIEP
                     DEIVTVNIKPAVDQSVQSLKEAGIVLTDFQKGNEKARERMKVQFSIASNRQGIVVGTD
                     HSAENITGFYTKYGDGAADIAPIFGLNKRQGRQLLAYLGAPKELYEKTPTADLEDDKP
                     QLPDEDALGVTYEAIDNYLEGKPVTPEEQKVIENHYIRNAHKRELAYTRYTWPKS"
     gene            complement(3671..5140)
                     /locus_tag="O2701_001892"
     CDS             complement(3671..5140)
                     /locus_tag="O2701_001892"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCZ4293778.1"
                     /translation="MYQLEDDSLMLHNDLYQINMAESYWNDNIHEKMAVFDLYFRKMP
                     FNSGYAVFNGLKRVIDFIEHFGFSESDLEYLKSIGYKDDFLSYLKDLKFTGSIRSMQE
                     GELCFGNEPLLRVEAPLIQAQLIETILLNIVNFHTLITTKASRIRQIASNDKLMEFGT
                     RRAQEIDAALWGARAAYIGGFDSTSNVRAGKLFGIPVSGTHAHAFVQTYGDEYVAFKK
                     YAERHKNCVFLVDTFHTLKSGVPNAIKVAKELGDKINFVGIRLDSGDIAYLSKEARRM
                     LDEAGFTETKIIASNDLDEETITSLKAQGAKVDSWGVGTKLITGYDQPALGAVYKLVA
                     IENEDGSYSDRIKLSNNAEKVTTPGKKNVYRIINKKTGKAEGDYITLENENPYDEQPL
                     KLFHPVHTYKMKFIKSFEAIDLHHNIYENGKLVYQMPTEDESREYLALGLQSIWDENK
                     RFLNPQEYPVDLSKACWDNKHKRIFEVAEHVKEMEEDNE"
     gene            5324..6400
                     /locus_tag="O2701_001893"
     CDS             5324..6400
                     /locus_tag="O2701_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase oxygenase"
                     /protein_id="HCZ4293779.1"
                     /translation="MLFKEAQAFIENMYKECHYETQIINKRLHDIELEIKETGTYTHT
                     EEELIYGAKMAWRNSNRCIGRLFWDSLNVIDARDVTDEASFLSSITYHITQATNEGKL
                     KPYITIYAPKDGPKIFNNQLIRYAGYDNCGDPAEKEVTRLANHLGWKGKGTNFDVLPL
                     IYQLPNESVKFYEYPTSLIKEVPIEHNHYPKLRKLNLKWYAVPIISNMDLKIGGIVYP
                     TAPFNGWYMVTEIGVRNFIDDYRYNLLEKVADAFEFDTLKNNSFNKDRALVELNYAVY
                     HSFKKEGVSIVDHLTAAKQFELFERNEAQQGRQVTGKWSWLAPPLSPTLTSNYHHGYD
                     NTVKDPNFFYKKKESNANQCPFHH"
     gene            6420..7214
                     /locus_tag="O2701_001894"
     CDS             6420..7214
                     /locus_tag="O2701_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HCZ4293780.1"
                     /translation="MQLYYLGPKGTFSYLACRQYFSENEATFQPKSNFFEVIKAVADD
                     DTSIGVVPIENSIEGTINIVADALAQQDVFAHGEIRLDINFALYGNGTDSISDIKKVY
                     SIAPAISQTTNYIHQHQFDYDYVDSTIQSLTKIENGVAAIAPLGSGEAYGFTPIDTHI
                     EDYPHNVTRFLVIKNQQQFDQNATSLMFLITPMHDKPGLLASVLNTFALFNINLSWIE
                     SRPLKTQLGMYRFFVQADSAITTDIKKVIAILETLDFKVEMIGAFN"
     gene            7286..7393
                     /locus_tag="O2701_001895"
     CDS             7286..7393
                     /locus_tag="O2701_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293781.1"
                     /translation="MVDFLLFVLFLHDAAVYVSLPLVQAIANVTFYFSF"
     gene            complement(7404..8966)
                     /locus_tag="O2701_001896"
     CDS             complement(7404..8966)
                     /locus_tag="O2701_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCZ4293782.1"
                     /translation="MAYFNQHQSMISKRYLTFFSKSKKKKPFSAGQLIGLILGPLLFL
                     LTLLFFHPQDLPWKGVYVLAITLWIATWWITEAIPIAATSLLPIVLLPLGHILTPEQV
                     SSEYGNDIIFLFLGGFILAIAMERWNLHTRVALTIINLIGASTSKILLGFMVATGFLS
                     MFVSNTAAVMIMIPIGLAIIKEAHDLQEANTNQTSIQKFEKSLVLAIGYAGTIGGLGT
                     LIGTPPLIILKGQYMQHFGYEISFAKWMIVGIPTVIVLLGITWLYLRYVAFRHDLKYL
                     PGGQTLIKQKLDELGKMKYEEKVVQTIFVLASLLWITREFLLKKWEVTSSVADGTIAI
                     FISILLFIIPAKNTEKHRRIIDWEVAKELPWGVLILFGGGLALAKGISESGLAKWLGE
                     QLKSLNGVSPILIVIVITIFVLFLTEVTSNTATATMILPILATLSVAVGVHPLLLMAP
                     AAMAANCAYMLPVGTPPNAIIFGSGKISIKQMASVGFWVNLISAIIIILVVYYVMPIV
                     LGIDINQPLPLK"
     gene            complement(9171..10268)
                     /locus_tag="O2701_001897"
     CDS             complement(9171..10268)
                     /locus_tag="O2701_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HCZ4293783.1"
                     /translation="MYINMKDYGLTGINKTKDTRAIQRALNRGRCKPTTVYIPKGTYD
                     ICKPLTIYGNTTLLLDNETILRRCHSGPLLKNGRRFGFYRGYNGHSHIHIKGGKFDMN
                     GVSYPYNNTAMCIGHAEDIQLIGVTIKNVVSGHAIDACGINGLYIKSCSFEGFIDYSG
                     ERFYSEAIQLDIQVPGAFPKFGTTDGTITKNVIIEDCYFGPSELPEMGSWNRAIGSHA
                     SRHNRYYENIHIRNNIFEDIQGYALTPLKYKDAFIINNKFINCEGGIRYLGVRDGKNA
                     ADVMTGKDLGSQAGINMNIIGNEFKGSMSKDAIHVRNYNNVKHKDVLIVGNTFNNSTQ
                     SIHLEDIDTVFLSPVEAGIQVTTINVDEIKK"
     gene            10640..11200
                     /locus_tag="O2701_001898"
     CDS             10640..11200
                     /locus_tag="O2701_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCZ4293784.1"
                     /translation="MTHRALLVVDYSYDFIADDGLLTCGKPGQNIEDFIVSRINDFNY
                     YQDHIFFLMDLHYLHDIHHPESKLFPPHNIVDTSGRELYGKVGKLYETIKAQPNVHFI
                     DKTRYDSFFGTPLDSLLRERSINQVEIVGVCTDICVLHTAISAYNLGYKISVPAEGVA
                     SFNQKGHEWALAHFKNSLGAEVEQHV"
     gene            11253..12182
                     /locus_tag="O2701_001899"
     CDS             11253..12182
                     /locus_tag="O2701_001899"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HCZ4293785.1"
                     /translation="MAKTYIFGHKNPDTDAISSAIIMAEFEQLRGNSGAKAYRLGDVS
                     AETQFALDTFNVPAPELLTDDLDGQDVILVDHNEFQQSSDTIASATIKHVIDHHRIAN
                     FETAGPLCYRAEPVGCTATILYKMFRERGFEIKPEIAGLMLSAIISDSLLFKSPTCTQ
                     QDVKAAEELKDIAKVDIQKYGLDMLKAGASTTDKSVEFLLNMDAKSFTMGDYVTRIAQ
                     VNAVDLDEVLNRKEDLEKEMLAVSAQEKYDLFVLVVTDIINSDSKILVVGAEKDKVGE
                     AFNVQLEDDMAFLSGVVSRKKQIVPQITEALTK"
     gene            complement(12377..12550)
                     /locus_tag="O2701_001900"
     CDS             complement(12377..12550)
                     /locus_tag="O2701_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293786.1"
                     /translation="MNVVAVNFKWSFELYMLKVENRAMNRVHNNIYNFNHNEKGRKKT
                     KIYTQHRKYFKKM"
     gene            12602..13981
                     /locus_tag="O2701_001901"
     CDS             12602..13981
                     /locus_tag="O2701_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCZ4293787.1"
                     /translation="MNIIEQKFYDSKAFFNTQQTKDISFRKEQLKKLSKAIKSYESDI
                     LEALYTDLGKNKVEAYATEIGITLKSIKIARKELKNWTKTKNVDTPLYLFPTKSYIKK
                     EPYGTVLIIAPFNYPFQLVFEPLIGAIAAGNTAIIKPSELTPNVARVIKRLINETFDA
                     NYIEVIEGGIEETQTLIHLPFDYVFFTGSENVGKIVYQAASENLVPVTLEMGGKSPVI
                     VDETANIKVASERICFGKFTNAGQTCVAPDYILVHESVKDDLITALSKTLREFYGQNI
                     QQSPDYGRIVNLKHYHRLTSLLNSAQMNIVFGGHSDEDERYIEPTLLDHVTSDSAIMQ
                     EEIFGPILPILTYQSLDEAIAFIHQRPKPLSLYLFSEDENATQRVINELSFGGGAIND
                     TLMHLANPKLPFGGVGASGMGRYHGKYSFDTFTHEKSYIFKSTRLESGVHLPPYKGKF
                     KYIKAFFKN"
     gene            complement(14101..15129)
                     /locus_tag="O2701_001902"
     CDS             complement(14101..15129)
                     /locus_tag="O2701_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HCZ4293788.1"
                     /translation="MTNGYIGSYTKKNGKGIYRFELNENQSRIDLLETGFELEASTYL
                     VRNNEVLYGINKEGEQCGVASLKIDDNGELHLLNKCLSSKAGTGCYVSISEDKRYLFE
                     AVYGAGIIRMYELNTHTGEIIRLIQELAHDFPTGTHERQDHPHAHYINQTPDGKYVAV
                     TDLGADRIVTYKFDDNGFEFYKESLFKDSDGTRHIEFHDNGKFAYVVHELSNTVSVAE
                     YNDGKFEELERHLTIPENFDGDTKLAAVRLSHDQQFLYVSNRGHDSIAIFKVLDNGQH
                     LELVTITESGGQFPRDFNIASSDDLLVCAHEQGDSVVTVFERNKETGKITLCDNTRVA
                     SEGVCVIF"
     gene            15399..15800
                     /locus_tag="O2701_001903"
     CDS             15399..15800
                     /locus_tag="O2701_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HCZ4293789.1"
                     /translation="MTSYEEETDYRNVPRELLDNNIPMGRGMIKWAPFATLPEQFETI
                     QQYIIDQNKITRPVLSDDQLAELNIRLHEALQYAQPVEVKFYNNGFVDSVRLTIYRID
                     AINYEIDGYVYNQQQRQKISIFDILDIVLLP"
     gene            complement(15818..15979)
                     /locus_tag="O2701_001904"
                     /pseudo
     CDS             complement(15818..15979)
                     /locus_tag="O2701_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5402 a         2796 c         2348 g         5433 t
ORIGIN      
        1 aaaggagttt ttatatgaaa agaaactttc caaaattaat agctttaagt ttaatatttt
       61 ctttaagtgt tacccctatt gcaaacgctg agagcaattc aaatatcaaa gcaaaagata
      121 agaagcatgt gcaagttaat gtcgaggaca agagtgttcc tactgacgta cgtaatttag
      181 cgcaaaaaga ctatctttct tatgttactt cacttgataa aatctataat aaagagaaag
      241 cctcgtatac acttggtgaa ccttttaaaa tttataaatt caacaaaaag agtgatggta
      301 attattattt tccagttttg aacactgaag gtaatatcga ttatatagtt acaatttcac
      361 caaaaatcac taagtattct agttcatctt ctaaatacac aatcaatgtt tctccatttt
      421 tatctaaagt cttaaatcaa tataaagatc aacaaattac tattttgaca aactcaaaag
      481 gttactatgt ggttactcaa aatcataaag ctaaactagt actaaaaaca ccacgtctag
      541 aagataaaaa gttaaaaaag actgagagta tacctactgg caataatgta acgcaactaa
      601 agcaaaaagc ttctgtcact atgccaacta gtcaatttaa aagtaataat tatacttaca
      661 acgagcaata tataaataaa cttgagaatt tcaagataag agaaactcaa ggtaacaatg
      721 gttggtgtgc aggctatacg atgtctgaat tactaaatgc gacttataat actaacaaat
      781 atcatgcaga agctgtgatg agatttttac acccaaactt acaaggacaa cgattccaat
      841 ttactggttt aacgccaaga gagatgattt attttggaca aactcaggga agaagtccac
      901 aacttcttaa tagaatgact acatataatg aagttgacaa tcttactaaa aataataaag
      961 gaattgctgt tttaggtagt agagtagaat caagaaatgg tatgcacgca gggcatgcca
     1021 tggcagttgt tgggaacgct aaattagata atggacaaga ggttataatt atatggaatc
     1081 catgggataa tggttttatg actcaagatg ctaaaaacaa tgttattcca gtatccaatg
     1141 gcgaccatta tcgatggtat tcatctattt atggttatta aaatttgtat tttagaaagg
     1201 taaataatat tatggaacaa attgaattat ttagtattga taaattcaaa tgtaattcag
     1261 aggctaagta ttatcttaat attattgagg gagaatggca tcctcaagat ttaaatgata
     1321 gtcctttaaa atttattctc agtacctcag acgattctga ttacatttgc aaatatataa
     1381 atacagaaca caaacaactc acattatata ataaaaataa tagctcaatt gttattgaaa
     1441 tatttatacc aaatgataat aaaatactac taacaattat gaacacagaa gctttgggaa
     1501 cttctcctag aatgactttc attaagcata agtcataaga ctatctagat atgattaagt
     1561 aaactattaa tcttattaaa ggtctctagg aagaaaaaat ttcatgcgat gagattgttt
     1621 tagaaaaagc gacgatatat ataatctaac aataattttt aaaatattaa gaacccccag
     1681 catatgcttt atatagcttg ctgggggttt ctcatacaat atggatcgag catcttgcat
     1741 gtataaacaa ataggctatt caaagtaaaa ttactcaaac tttgaatagc ctttcctctt
     1801 caaatagtct ttatttaaat tggattatgt tattggattt tcttacctac aaaaacgatt
     1861 tcttgatgtg atacttcaac cgtgccatct ttttgctctt gaaacacggg tttttcaata
     1921 cgttttaccg gcatataccc ttgcattttc attcgtgaca aacaatcagc aattgtttca
     1981 ttctcattta ctttgaattt catgttctac tacactttcc agttcttctg gttcataatc
     2041 tttaagcttt ctacgacgaa tgcctttagt ccagaatcca ccatgtatgt ttcgaggttc
     2101 atacgcaatg ataaatgctt tcggatctaa attttttatc gtatccatca atttacgttc
     2161 atattttctt ggtgttaaaa tttgcatcac catacgacta ccatctctac caaacgcagc
     2221 atagtgcgta acgccatatc ctaaatttcg aagttcattc ggtaaatcta actcatattc
     2281 tgctgaagtt acatttacaa ctgtataacc taatgccagt ttttcttcta ttttcatacc
     2341 aacaatgata cctattgaaa aaccaaatgc gtaggcaata atattttgaa tatggtctaa
     2401 attagacata accaaaccta aaccaacgat ataaactaat acttctaaaa aactaactga
     2461 tgcagcaata taacgataac ctttcaacgt taaaattgtt cgcatcgtta aaaacgttac
     2521 ataacaaatg ttaatgataa atatagttag taccattaac catggatttt ctgttacgaa
     2581 tgacataagg cgccccactc tctttcaatt acactttaaa taccaaactg cttcaaccta
     2641 accttaatga atgcatatta atataatata gtacgaaaac tctcataaat aaatagcgaa
     2701 tttgtcataa ataaaaattt attctcaaat gaacatactt actttagttc atacactttc
     2761 aatttataca ctgaagtagt ctagctgaat gtggtttaat gttattaatt ctaactcata
     2821 tttaatttaa gtcacacgtt agaagaaaaa attaaattag gattttggcc acgtgtatct
     2881 tgtatatgca agttcacgtt tgtgtgcatt tcgtatataa tgattttcaa ttactttttg
     2941 ttcttctggc gtaactggct taccttctaa ataattatca atcgcctcat aagttacacc
     3001 taatgcatct tcatctggaa gctgtggttt atcatcttct aaatcagcag ttggcgtttt
     3061 ttcatataat tcctttggcg caccaagata cgctaataat tgacgacctt gtcgtttatt
     3121 caaaccaaat ataggtgcga tatctgcagc accatcaccg tacttcgtat aaaacccagt
     3181 tatattttca gctgaatgat ctgttcctac tacaatacct tgtcggtttg aagcaattga
     3241 aaattgtact ttcatacgtt cacgcgcttt ttcatttcct ttttggaaat ctgtaagaac
     3301 aataccggct tcttttaatg attgcacact ttgatcaact gcaggcttaa tattgactgt
     3361 tactatttca tctggttcaa tgaatcgcaa agcttgctca acttcatcag catctttttg
     3421 aactccataa ggtaatttaa ctgcaataaa cgtacaatca atgccttctt cacgtaattc
     3481 gttaacagac atttgtacta gttttccaac taatgtagaa tcctgtcctc ctgaaatacc
     3541 taacactaaa gattttataa atgaatgtga ttgtacataa ttttttataa attgctttaa
     3601 ttccataatt tcttcagcac tatcgatacg ctttttcact ttcatttctt gtacaataac
     3661 gtcttgtaat ttactcatta tcttcttcca tctccttaac gtgttccgca acttcaaaaa
     3721 tacgtttatg tttattatcc caacatgcct tgcttaaatc gactggatat tcttgtggat
     3781 tcaggaaacg cttattttca tcccaaatag attgtaatcc tagtgctaaa tattcacgtg
     3841 attcatcttc tgttggcatt tgatatacta atttaccatt ttcataaata ttatgatgca
     3901 aatcaatggc ttcgaaagat tttataaatt tcattttata agtatgcact ggatggaata
     3961 attttaaagg ttgttcatcg tatggatttt cattttccaa agtaatataa tcgccttctg
     4021 ccttacctgt tttcttgttt ataatgcgat atacattttt cttacctggc gtcgtaacct
     4081 tttcagcgtt atttgataat ttaatacgat cactatatga accatcttca ttttcaatag
     4141 ctacaagttt atatactgca cctaatgctg gttgatcgta tcctgtaatc agctttgtac
     4201 caacgcccca agaatctact tttgcacctt gtgctttcaa actcgtaatc gtttcttcat
     4261 ccaaatcatt agacgcgata attttagttt cagtaaatcc tgcttcatca agcatacgtc
     4321 ttgcctcttt agataaataa gcgatatctc cagaatctaa tcgaatacct acaaagttaa
     4381 ttttgtcacc taattctttt gcaactttta ttgcatttgg cacgccagat tttaaagtat
     4441 ggaatgtatc tactaggaac acacaatttt tatgtctttc agcatatttt ttgaaggcaa
     4501 catattcgtc tccataagtt tggacaaatg catgtgcatg tgtaccagac acaggtatac
     4561 caaataattt ccccgcccta acattacttg tagaatcaaa gcccccgatg taagcagctc
     4621 tagcgcccca caatgctgca tcaatttctt gcgcacgacg tgtaccaaac tccattaatt
     4681 tatcatttga tgcaatttga cgaattctgc tagcctttgt tgtaattaat gtatggaaat
     4741 ttacaatgtt taataaaatt gtttctatta attgcgcttg aatcaatggt gcttctacgc
     4801 gtaacaatgg ttcgttacca aagcataatt cgccttcttg catcgaacgg atgctgcctg
     4861 tgaattttaa atcttttaaa tatgataaga aatcatcctt gtagccaata gactttaaat
     4921 attccaaatc agattctgaa aatccaaaat gttctataaa atcaatgacg cgttttaaac
     4981 cattaaaaac agcatagcca ctattaaatg gcatttttct aaaatacaaa tcaaatacag
     5041 ccattttttc atgaatatta tcattccaat aactttcagc catatttatt tgatataagt
     5101 cattatgtaa cattaaactg tcgtcttcta attggtacac ttgtatctct ccaatcgacc
     5161 taaatatttt cttacatttt atcataattc atttttttat atacataaga gccccttaat
     5221 ttccatactt ttaattaaaa tcaaccaaca atttaatgac atatacataa tttttaagag
     5281 tattttaata atgtagacta taatataaag cgaggtgttg ttaatgttat ttaaagaggc
     5341 tcaagctttc atagaaaaca tgtataaaga gtgtcattat gaaacgcaaa ttatcaataa
     5401 acgtttacat gacattgaac tagaaataaa agaaactggg acatatacac atacagaaga
     5461 agaacttatt tatggtgcta aaatggcttg gcgtaattca aatcgttgca ttggtcgttt
     5521 attttgggat tcgttaaatg tcattgatgc aagagatgtt actgacgaag catcgttctt
     5581 atcatcaatt acttatcata ttacacaggc tacaaatgaa ggtaaattaa agccgtatat
     5641 tactatatat gctccaaagg atggacctaa aattttcaac aatcaattaa ttcgctatgc
     5701 tggctatgac aattgtggtg atcctgctga aaaagaagtt acacgcttag caaatcactt
     5761 aggttggaaa ggaaaaggta ctaattttga cgtgttacca ctgatttacc aattacctaa
     5821 tgagtcagtt aaattttacg aatatcctac ttcattaatt aaagaagtac ctattgaaca
     5881 taatcattat ccaaaattaa gaaaattgaa cttaaaatgg tatgcagtcc ctatcatttc
     5941 caatatggac ttaaaaatcg gtggcattgt atatccaact gcacccttta acggttggta
     6001 tatggtaact gaaattggcg tacgtaactt tattgatgat taccgttaca atttactaga
     6061 aaaagttgca gatgcgtttg aatttgatac acttaaaaat aattcattta ataaagatcg
     6121 agcacttgtt gaattgaact atgctgtgta tcattccttt aaaaaagaag gcgtatcaat
     6181 tgtcgatcat ttgaccgctg caaagcaatt cgaactattc gaacgtaacg aagcacaaca
     6241 aggtcgtcaa gttaccggaa aatggtcttg gctagcaccg ccattatctc caacattgac
     6301 gtcaaattat catcacggat atgacaatac agtaaaagat ccaaactttt tctataaaaa
     6361 gaaagaatca aatgctaacc agtgcccttt ccatcattaa gttagtagag gtgtagcata
     6421 tgcaattata ttatttaggc cctaaaggta cattttcata tttggcttgt cgtcagtatt
     6481 tttctgaaaa tgaagcaact tttcaaccaa agtcaaattt ttttgaagtc atcaaggcag
     6541 ttgcagatga tgacacatcg attggtgttg tccctattga aaattctata gaaggaacaa
     6601 tcaacattgt ggcagatgca cttgcacagc aagatgtctt tgcacacggt gaaatacgtt
     6661 tagatattaa ctttgcatta tatggtaatg gaacagattc catttctgat attaaaaaag
     6721 tgtattctat tgcaccagca atcagtcaaa caacaaatta catacatcaa catcaatttg
     6781 actatgatta tgtcgacagt acaattcaaa gtttgactaa aattgaaaat ggcgttgcag
     6841 caattgcacc tttaggtagt ggcgaagctt atggttttac acctattgat acacatattg
     6901 aagactatcc acataacgtg acacgttttt tagttataaa aaatcagcag caatttgatc
     6961 aaaatgcgac atcattgatg tttttaatta caccaatgca tgataaacct gggttgttag
     7021 caagtgtgct taatacattt gctttgttta atattaattt atcctggatt gagtctcgtc
     7081 ctttaaaaac acaattaggt atgtatcgat ttttcgttca ggctgattct gcaattacaa
     7141 ccgatataaa gaaagtcatt gctattttag aaaccttaga tttcaaagta gaaatgattg
     7201 gtgcttttaa ttaaacttta ttttctaggt tctacaattt aaataaaata aagagtcaac
     7261 caaggattaa aataggaatc catccttggt tgactttctt ttgtttgtat tgtttttaca
     7321 cgatgctgcg gtatacgttt cgttaccact cgtccaagca attgctaatg taacctttta
     7381 tttttcgttc taatctgcaa ttactatttc aatggcagtg gttgatttat atcaatacct
     7441 aaaactatag gcattacata atacacgact aaaataataa ttattgcact gattaagttt
     7501 acccagaatc ctactgatgc catttgtttg atagatattt taccagaacc aaagataatt
     7561 gcattcggtg gtgtccctac tggtaacatg tatgcacagt tagccgccat agctgcaggt
     7621 gccataagta gtaatggatg cactccaaca gcaacagaca acgttgctaa aatcggtaaa
     7681 atcatcgttg cagttgcagt attagatgtc acttcagtta aaaataagac aaagattgtt
     7741 atgacaatta caataagaat cggactaaca ccatttaatg atttcaactg ttcgcctaac
     7801 cattttgcta aaccactttc agaaatacct ttcgctaatg ctaaaccgcc accaaataaa
     7861 attaatacac cccaagggag ctcttttgca acttcccagt caatgatacg gcgatgtttt
     7921 tcagtatttt tagctggaat aataaataat aatattgata taaaaatagc aatcgtacca
     7981 tctgcaacag atgacgtaac ttcccatttt ttcagaagaa actctcttgt aatccataat
     8041 aagctagcaa gtacaaagat agtttgtact accttttctt catacttcat tttgccaagc
     8101 tcgtctaact tttgtttaat taacgtctga ccaccaggta aatatttcaa atcatgtcta
     8161 aacgcaacat atcttaaata gagccaagta atacctaaca aaacaatgac cgttggaatc
     8221 cctacaatca tccatttagc aaaactaatt tcatatccaa aatgttgcat gtattgtcct
     8281 tttaaaataa ttaatggcgg ggttccgatt aatgtaccca agccaccaat cgtacctgca
     8341 tagccaattg ctaaaactag agatttttca aacttttgaa tacttgtttg attcgtattg
     8401 gcttcttgta aatcatgtgc ttccttaata attgctaaac caatcggaat cataatcatt
     8461 acagctgcag tgttcgatac aaacatagat aagaatcctg ttgccaccat gaatccaagt
     8521 aatatttttg aagtgcttgc accaatcaaa ttaataattg tcaacgctac tctcgtatgt
     8581 aaattccatc tttccattgc aattgccaaa ataaatccac ctaaaaacaa aaagataata
     8641 tcattgccat attcggatga tacttgttct ggtgtaagta tatgacctaa tggtaataac
     8701 acaattggta ataagctcgt tgctgcaata ggaattgctt cagtaatcca ccaagtcgca
     8761 atccataaag taatcgctaa aacatagacg cctttccaag gtaagtcttg tggatgaaag
     8821 aataataatg ttaataggaa aagtaatgga cctaatatta gtccaataag ttgtcccgca
     8881 ctaaacggtt tctttttctt tgattttgaa aagaatgtta aatacctttt cgatatcatt
     8941 gattgatgtt gattgaaata agccataaat ttccccctca tgatgattat gacaagctac
     9001 ttcaactcac tatattagag tatagaaagt tgtctatctt gtattatcat taccgtcaaa
     9061 ataccccttt tatcagataa ttgggtttat tatagcatat ttataccgtt ttatgaatgc
     9121 gctttcaaaa attatctact attcttaatc ctaatgtcat gcgaaacttt ttactttttt
     9181 atttcatcta cattgattgt agtaacttga atacccgctt caacaggact taaaaacact
     9241 gtatcaatat cttctaaatg aattgattga gtcgaattat tgaatgtatt cccaacgatt
     9301 aatacatctt tatgtttaac attattataa ttacgtacat gtatcgcatc tttagacatt
     9361 gatcctttaa attcatttcc aattatattc atatttatgc ctgcttggga acctaagtct
     9421 tttcctgtca tcacatctgc tgcattttta ccatctctaa ctcctaaata tctaatgcca
     9481 ccctcacagt taataaactt attattaata atgaaagcat ctttatactt caagggagtt
     9541 aatgcataac cttgtatatc ttcaaatata ttatttctaa tatgaatatt ctcatagtat
     9601 cgattatgtc tacttgcatg tgagccaata gcacgattcc aacttcccat ttcgggcaat
     9661 tctgaaggtc caaaataaca atcttcgata atgacatttt tcgttatcgt accatctgtc
     9721 gttccgaatt ttggaaaagc accaggtact tgaatgtcta attgtattgc ttcagaataa
     9781 aagcgttcgc cactatagtc tatgaatcct tcaaatgaac agcttttaat atagagtccg
     9841 ttaatcccac aagcatcaat tgcatgacca ctcactacat tcttaatggt cacacctatt
     9901 aattgaatat cttcagcatg cccaatgcac atagctgtat tgttataagg atacgataca
     9961 ccattcatat caaacttgcc gcctttaata tgaatatgac tgtgtccatt ataaccacga
    10021 taaaaaccaa agcgacgacc attttttaat aaaggaccag aatgacatcg gcgtaaaata
    10081 gtttcattat ctaacaaaag tgttgtattg ccatatatcg ttaatggttt gcaaatatca
    10141 tacgtccctt tcggtatata aactgtcgtt ggtttacatc ttccacgatt taacgcacgt
    10201 tgtattgctc gagtatcttt agttttgttt atgcctgtta acccataatc tttcatattg
    10261 atatacataa atcgtcatcc tttgtctatt ttctaattac tcatatatta accaattatt
    10321 caaattataa tcgtgttagg ttgatttaac agatagtcat aaatctcgtg atgccatcta
    10381 ttagcatttt tatattcaaa acatcaacac ttgatattag ttctaagttt gtcacattta
    10441 gtattctatt acagctattt taaaactatc gcaaaaactt cttatattat ttgttgattg
    10501 cgtgatttgc tcttccaaaa aagttgatta atctatatac ttaatctata tttatatatt
    10561 aaccattagg gttaaaaatt actctagcat ttatgaatag atgggagttt attttattat
    10621 tatataggag agatgttgaa tgacacatcg cgcactatta gttgttgact attcatatga
    10681 ctttatcgca gacgacggct tactaacatg cggtaaacct ggacaaaata ttgaagattt
    10741 tattgtttct cgtatcaatg actttaatta ttatcaagac catatattct ttttaatgga
    10801 tttacattat ttacatgaca ttcatcatcc tgaaagtaaa ttattcccac cacacaatat
    10861 cgtagataca agtggtagag aattatacgg taaagtaggt aaattatacg aaacaattaa
    10921 agcgcaacct aatgtacatt tcattgataa aacgcgctat gattcgttct ttggtacccc
    10981 gcttgatagt ttattgagag aaagaagtat taatcaagtc gaaatcgttg gtgtatgtac
    11041 cgatatttgc gtgttacata cagcaatttc tgcatacaac ttaggttata aaatttcagt
    11101 acctgctgag ggagtggctt catttaatca aaaagggcat gaatgggcac ttgcacattt
    11161 caaaaactca ttaggtgcag aggtagaaca acacgtttaa atcgtgctaa aataattata
    11221 aagaatacaa tttacaaggg agatatttga caatggctaa aacatatatt ttcggacata
    11281 agaatccaga cactgatgca atttcatctg cgattattat ggcagaattt gaacaacttc
    11341 gaggtaattc aggagccaaa gcataccgtt taggtgatgt gagtgcagaa actcaattcg
    11401 cgttagatac atttaatgta cctgctccgg aattattaac agatgattta gatggtcaag
    11461 atgttatctt agttgatcat aacgaattcc aacaaagttc tgatacgatt gcctctgcta
    11521 caattaagca tgtaattgat catcacagaa ttgcaaattt cgaaactgct ggtcctttat
    11581 gttatcgtgc tgaaccagtt ggttgtacag ctacaatttt atacaaaatg tttagagaac
    11641 gtggctttga aattaaacct gaaattgccg gtttaatgtt atcagcaatt atctcagata
    11701 gcttactttt caaatcacca acatgtacac aacaagatgt taaagcagct gaagaattaa
    11761 aagatattgc taaagttgat attcaaaagt acggcttaga tatgttaaaa gcaggtgctt
    11821 caacaactga taaatcagtt gaattcttat taaacatgga tgctaaatca tttactatgg
    11881 gtgactatgt gactcgtatt gcacaagtta atgctgttga ccttgacgaa gtgttaaatc
    11941 gtaaagaaga tttagaaaaa gaaatgttag ctgtaagtgc acaagaaaaa tatgacttat
    12001 ttgtacttgt tgttactgac atcattaata gtgattctaa aattttagtt gtaggtgctg
    12061 aaaaagataa agttggcgaa gcattcaatg ttcaattaga agatgacatg gccttcttat
    12121 ctggtgtcgt ttctcgaaaa aaacaaatcg tacctcaaat cactgaagca ttaacaaaat
    12181 aatactatat tactgtctaa ttatagacat gttgtattta actaacagtt cattaaagta
    12241 gaatttattt cactttcaat gaactgtttt ttatttacgt ttgactaatt tacaaccctt
    12301 tttcaatagt agtttttatt cctttagcta ccctaaccca cagattagtg atttctatac
    12361 aattcccctt ttgtctttac attttcttaa aatatttgcg atgttgagta taaatttttg
    12421 ttttcttcct acctttttcg ttatgattaa agttataaat attattatgt acacgattca
    12481 tcgctctatt ttcaactttc aacatatata attcgaaaga ccatttaaaa ttaacggcca
    12541 caacattcaa atcaattaat cgctttttcc aaaataatca tataaggagg ttcttttcat
    12601 tatgaatatc attgagcaaa aattttatga cagtaaagct tttttcaata cacaacaaac
    12661 taaagatatt agttttagaa aagagcaatt aaagaagtta agcaaagcta ttaaatcata
    12721 cgagagcgat attttagaag cactatatac agatttagga aaaaataaag tcgaagctta
    12781 tgctactgaa attggcataa ctttgaaaag tatcaaaatt gcccgtaagg aacttaaaaa
    12841 ctggactaaa acaaaaaatg tagacacacc tttatattta tttccaacaa aaagctatat
    12901 caaaaaagaa ccttatggaa cagttttgat cattgcacca tttaactatc cttttcaact
    12961 agtgttcgaa cctttaatcg gtgctattgc agcaggtaat acggcaatta ttaaaccatc
    13021 tgagttgacg cctaatgttg cacgagtgat taaacggtta atcaatgaaa catttgatgc
    13081 aaattacatt gaagttattg agggaggaat tgaagaaacg caaacgttaa ttcacttacc
    13141 ttttgactat gtcttcttta caggaagtga aaatgtaggc aaaatcgttt atcaagctgc
    13201 cagcgaaaat ttagttcctg tgacattaga aatgggcggt aaatctccag tcatcgttga
    13261 tgaaacagcg aatattaaag ttgctagtga gcgcatttgt tttgggaaat tcactaatgc
    13321 cggccaaaca tgtgttgcac cagattacat tttagtacac gaatctgtaa aagatgattt
    13381 aatcacagcc ctatcaaaaa cgttgcgtga attttatggt caaaatatac aacaaagtcc
    13441 agattatggc cgcattgtaa accttaaaca ttatcatcgt ctgacttcat tacttaacag
    13501 tgcacaaatg aatattgtat ttggtggtca tagtgatgag gatgaacgtt atatagaacc
    13561 aacattgtta gatcacgtta caagtgattc agcaattatg caagaagaaa tttttggtcc
    13621 tatcttaccg attttaacgt atcagtcatt ggatgaagca atagccttta ttcaccaaag
    13681 accaaaacct ttgagtttat atttatttag cgaagatgaa aatgctacac aacgtgtaat
    13741 aaacgagcta tcatttggcg gcggcgctat taatgataca ttgatgcacc tagcgaatcc
    13801 taaattacca tttggtggtg ttggcgcctc aggtatggga cgctatcatg gtaaatattc
    13861 attcgacact tttacacatg aaaaaagcta cattttcaaa tctacacgat tagaatcagg
    13921 tgtccattta ccaccatata aaggtaaatt taaatacatc aaagctttct ttaaaaatta
    13981 atttttgaga caaaaataaa ccatgcttcc ataatttgat gttcttgaaa acaataaatt
    14041 tcaaggtata cacaaatttt ttggaaacat ggttttcttt ttatcatgat taattaaaga
    14101 ttaaaatatg acacatacac cttcagatgc tacacgagtg ttatcacata gcgtaatttt
    14161 acctgtttct ttatttcttt cgaaaacagt tacaactgaa tctccttgct catgagcaca
    14221 aactaaaagg tcatctgatg aggcaatatt aaaatctctt gggaattgac caccactttc
    14281 agtaattgtt actagttcta agtgttgacc attatcaaga actttaaaaa ttgcaatgct
    14341 atcatgccct ctattagata catataagaa ttgttgatca tgagataaac gcactgctgc
    14401 aagtttagta tctccatcaa agttttcagg aattgttaaa tgacgctcga gctcttcaaa
    14461 tttaccgtca ttatattctg caacactcac agtatttgat aattcgtgta cgacataagc
    14521 aaattttcca ttatcatgaa attcaatatg tcttgtccca tcactatctt taaataaaga
    14581 ttctttataa aattcaaacc cgttgtcatc aaatttataa gtaacgatac gatcagcacc
    14641 taaatctgtt actgcaacgt acttaccatc tggagtttga ttaatataat gtgcgtgtgg
    14701 atgatcttgt ctttcatgtg tacctgttgg aaaatcatgt gcaagttctt gaattagacg
    14761 tataatttca cctgtgtgcg tatttaattc atacatacgt atgatgccag caccatatac
    14821 cgcttcaaat aaatatcgtt tatcttctga aatcgataca taacaacctg taccagcttt
    14881 tgaagacaaa catttattta ataaatgcaa ttcaccattg tcatcaattt tcaaacttgc
    14941 aacaccgcat tgttctcctt ctttgttgat tccatataaa acttcattat tacgcaccaa
    15001 atatgtagac gcttctaatt caaatcctgt ttctaataaa tcaatacgtg actgattttc
    15061 gtttaattca aaacgataaa tccctttacc attcttttta gtgtaagaac caatatatcc
    15121 atttgtcatt acttttaccc ctccatgtta gtcttatttt cattttatta aattttagct
    15181 agaattaaca atcatttatc atttccttaa agctaaaact atttaactac tttaaaagaa
    15241 ctatttgctg tgatatgtca ttattcgatt ttaattaatc cctctttttt acttacacga
    15301 gcattaatta ttaacgcgtt tatgcttcca caaaattttt gttgcaaaat agaacacatg
    15361 ttcgtataat gtaattatca gattggggtg aattaaggat gacaagttat gaagaagaaa
    15421 ctgattatcg taacgttcca agagaattac tagataacaa tatacctatg ggaagaggca
    15481 tgattaaatg ggctcctttt gcaacattac ccgaacaatt cgaaacgatt caacaataca
    15541 ttatcgacca aaacaaaatt acacgtcctg tattatcaga tgatcaattg gctgagctaa
    15601 acattcggct acacgaggcg ctacaatatg cccaacctgt agaagttaaa ttttataata
    15661 atggctttgt agattcagta cgcttaacca tttatcgtat tgatgctatt aattatgaaa
    15721 ttgatggtta tgtttacaat cagcaacaac gtcaaaaaat ttctatcttt gatatcttag
    15781 atattgtctt gttaccttaa tgctttgaac ataattaaac agattgagta tatgcatata
    15841 agatgatttt taacatcatt tttggatgat aggatgttgc gccacgatga tgtctgaatt
    15901 catcgaattc gctatcaggt attgtttcaa caatttcatt aacatatcgt gaaatatcat
    15961 tttgagggat tctaacaga
//
LOCUS       DALAGD010000072        15525 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.073, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000072 DALAGD010000000
VERSION     DALAGD010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15525
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.073"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            114..398
                     /gene="groES"
                     /locus_tag="O2701_001905"
     CDS             114..398
                     /gene="groES"
                     /locus_tag="O2701_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCZ4293790.1"
                     /translation="MLKPIGNRVIIEKKEQEQTTKSGIVLTDSAKEKSNEGVIVAVGT
                     GRLLNDGTRVTPEVKEGDRVVFQQYAGTEVKRDNETYLVLNEEDILAVIE"
     gene            474..2090
                     /gene="groL"
                     /locus_tag="O2701_001906"
     CDS             474..2090
                     /gene="groL"
                     /locus_tag="O2701_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500736.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCZ4293791.1"
                     /translation="MVKQLKFSEDARQAMLRGVDQLANAVKVTIGPKGRNVVLDKEFT
                     APLITNDGVTIAKEIELEDPYENMGAKLVQEVANKTNEIAGDGTTTATVLAQAMIQEG
                     LKNVTSGANPVGLRQGIDKAVKVAVEALHENSQKVENKNEIAQVGAISAADEEIGRYI
                     SEAMEKVGNDGVITIEESNGLNTELEVVEGMQFDRGYQSPYMVTDSDKMVAELERPYI
                     LVTDKKISSFQDILPLLEQVVQSNRPILIVADEVEGDALTNIVLNRMRGTFTAVAVKA
                     PGFGDRRKAMLEDLAILTGAQVITDDLGLDLKDASIDMLGTASKVEVTKDNTTVVDGD
                     GDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKL
                     RIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIEAEGDIETGVNIVLKALTAPVRQ
                     IAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNMLEEGIVDPTKVTRSALQHAASVA
                     AMFLTTEAVVASIPEKNNDQPNMGGMPGMM"
     gene            complement(2185..2731)
                     /locus_tag="O2701_001907"
                     /pseudo
     CDS             complement(2185..2731)
                     /locus_tag="O2701_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002476300.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            2792..3004
                     /locus_tag="O2701_001908"
     CDS             2792..3004
                     /locus_tag="O2701_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island family protein"
                     /protein_id="HCZ4293792.1"
                     /translation="MTDDNVNDHIIKNHIEMIVDRLATDKEFYIFDSLIQGLSYQDIS
                     SALDCSEQSVILWYETILDKIVGVIK"
     gene            3001..3591
                     /locus_tag="O2701_001909"
     CDS             3001..3591
                     /locus_tag="O2701_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCZ4293793.1"
                     /translation="MSELTAKQARFVNEYIRTLNVTQSAIKAGYNANSAHVTACRLLK
                     KPHIKQYIQEQKDKIIDENVLTAKELLHVLTNAAVGDETETKEVVVKRGEYKENPQSG
                     KVQLVYNEHVELIEVPIKPSDRLKARDMLGKYHKLFTDKHDINGNVPIFINIGEWDGG
                     DEGLDKAVKDVSNDNPNHTVIVDDIPLEDYESIRFL"
     gene            3909..4286
                     /locus_tag="O2701_001910"
     CDS             3909..4286
                     /locus_tag="O2701_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293794.1"
                     /translation="MFISLAIWSINSMGNKQCISLNLLLSDDTFAVISVMTTLGTAIL
                     GGIAPSIYGIFTSPIDKKSSFRAISDWEQEQRNNVKDLMMLLPFTLLSILIVIFIFQF
                     AYSKLFNTIAIIIFIIILVVMFF"
     gene            4451..4894
                     /locus_tag="O2701_001911"
     CDS             4451..4894
                     /locus_tag="O2701_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4293795.1"
                     /translation="MSIITRLFNNSDFEKLNQLCKLYDDLGYPTNENDLKKRLKKITN
                     HDDYFLLLLIKENKIIGLSGMCKMMFYEKNAEYMRILAFVIHSEFRKKGYGKRLLADS
                     EEFSKRLNCKAITLNSGNRNERLSAHKLYSDNGYVSNTSGFTKQL"
     gene            complement(5747..7054)
                     /locus_tag="O2701_001912"
     CDS             complement(5747..7054)
                     /locus_tag="O2701_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCZ4293796.1"
                     /translation="MNKVHKPLYFYLMLFFSTTIIGALLLYLPFTGKKPISFLDALFI
                     ASSAFTVTGLSPVDIGSQFNILGEIVILLLIQIGGLGIVTVTLLTLVFLNRKISMKNR
                     FLIMVTWNIDEPGGVIKLIKHLAIYSLVTELIGMICLCLSFIPKFGIGKGLFLSLFTS
                     VSAFNNAGFALFKNNLIDYSSDPIVIITISILIIFGGIGHFVVIDFINCKKLSKLSLH
                     SKLVLTTTSILIIIGAITFFLLEQFNTMQHMGLVEKIGNSFFQSVTTRTAGFNSIDIA
                     SINKSTALMLMLLMFIGGAPLSAAGGIKITTFAVAFIFVLNYIRKENNVSVFNKEISD
                     KHIKLSIVTINISFLFISIITFILSIINPNISLIKLLFEVVSAFGTVGLSMNLTTEYH
                     GITKIIIIFVMLCGKVGLLTLLRTFIPPKSPKNYRYTKGQIYL"
     gene            7215..8126
                     /locus_tag="O2701_001913"
     CDS             7215..8126
                     /locus_tag="O2701_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-hydroxamate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCZ4293797.1"
                     /translation="MKRLIGILLCNLFILTACSASVDKTSNSTKTTDYKIENGETLKV
                     PEKPKRVAVLTGFYVGDFIKLGIKPIAVSDITKDSSILKPYLKGVDYIGENDVERVAK
                     AKPDLIVVDAMDKNIKKYQKIAPTIPYTYNKYNHKEILKEIGKLTNNEDKAKKWIEEW
                     DDKTRKDKKEIQSKIGQATASVFEPDEKQIYIYNSTWGRGLDIVHDAFGMPMTKQYKD
                     KLQEDKKGYASISKENISKYAGDYIFLSKPSYGKFDFEKTHTWQNIEAVKKGHVISYK
                     AEDYWFTDPITLEHLRSKLKKEILNKK"
     gene            8188..9033
                     /locus_tag="O2701_001914"
     CDS             8188..9033
                     /locus_tag="O2701_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCZ4293798.1"
                     /translation="MKQLVGIPESMLIPLIARAKEYENEKPIIKDALSKKIFDGLDDM
                     YKNVTCDDMSQIGISIRTVIIDCVTKRLIKDNKDLIVVNIGCGLDTRFQRFNKEKISW
                     IDLDVPESIEIRKTFFKESNSYKMISKSMLDYSWIDDVKNYKFFNSKSNILFIIEGVL
                     MYFDESVMTQLLDTIIKKMGDHNLTFAIEFCSKTIANNTKRHQSVSKLSSQPVFKYGY
                     NDLKKLNEILPNTIRVIHEYNYFDYYKNRWGLFGYCRFIPYLKKRLNNKIVLMKYKVP
                     KRQRH"
     gene            complement(9405..10628)
                     /locus_tag="O2701_001915"
     CDS             complement(9405..10628)
                     /locus_tag="O2701_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HCZ4293799.1"
                     /translation="MTTFSEKEKIQLLADIVELQTENNNEIDVCNYLTDLFDKYDIKS
                     EILKVNEHRANIVAEIGNGSPILALSGHMDVVDAGNQDNWSYPPFQLTEKDGKLYGRG
                     TTDMKGGLMALVVSLIELKEQNELPHGTIRLLATAGEEKEQEGAKLLADKGYLDDVDS
                     LIIAEPTGSGIYYAHKGSMSCKVTATGKAVHSSVPFIGDNAIDTLLEFYNLFKEKYSE
                     LKQQDTKHELDVAPMFKSLIGKEISEEDANYASGLTAVCSIINGGKQFNSVPDEASLE
                     FNVRPVPEYDNDFIESFFQNIINDVDSNKLSLDIPSNHRPVTSDKNSKLITTIKDVAS
                     SYVEQDEIFVSALVGATDASSFLGDNKDNVDLAIFGPGNPLMAHQIDEYIEKDMYLKY
                     IDIFKEASIQYLKEK"
     gene            11063..12118
                     /gene="lukH"
                     /locus_tag="O2701_001916"
     CDS             11063..12118
                     /gene="lukH"
                     /locus_tag="O2701_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit H"
                     /protein_id="HCZ4293800.1"
                     /translation="MKNKKRVLIASSLSCAILLLSAATTQANSAHKDSQDQNKKEHVD
                     KSQQKDKRNVTNKDKNSTAPDDIGKNGKITKRTETVYDEKTNILQNLQFDFIDDPTYD
                     KNVLLVKKQGSIHSNLKFESHKEEKNSNWLKYPSEYHVDFQVKRNRKTEILDQLPKNK
                     ISTAKVDSTFSYSSGGKFDSTKGIGRTSSNSYSKTISYNQQNYDTIASGKNNNWHVHW
                     SVIANDLKYGGEVKNRNDELLFYRNTRIATVENPELSFASKYRYPALVRSGFNPEFLT
                     YLSNEKSNEKTQFEVTYTRNQDILKNRPGIHYAPPILEKNKDGQRLIVTYEVDWKNKT
                     VKVVDKYSDDNKPYKEG"
     gene            12140..13156
                     /gene="lukG"
                     /locus_tag="O2701_001917"
     CDS             12140..13156
                     /gene="lukG"
                     /locus_tag="O2701_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit G"
                     /protein_id="HCZ4293801.1"
                     /translation="MIKQLCKNITICTLALSTTFTVLPATSFAKINSEIKQVSEKNLD
                     GDTKMYTRTATTSDSQKNITQSLQFNFLTEPNYDKETVFIKAKGTIGSGLRILDPNGY
                     WNSTLRWPGSYSVSIQNVDDNNNTNVTDFAPKNQDESREVKYTYGYKTGGDFSINRGG
                     LTGNITKESNYSETISYQQPSYRTLLDQSTSHKGVGWKVEAHLINNMGHDHTRQLTND
                     SDNRTKSEIFSLTRNGNLWAKDNFTPKDKMPVTVSEGFNPEFLAVMSHDKKDKGKSQF
                     VVHYKRSMDEFKIDWNRHGFWGYWSGENHVDKKEEKLSALYEVDWKTHNVKFVKVLND
                     NEKK"
     gene            complement(13394..14224)
                     /gene="sph"
                     /locus_tag="O2701_001918"
                     /pseudo
     CDS             complement(13394..14224)
                     /gene="sph"
                     /locus_tag="O2701_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239616.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
     gene            complement(14275..15312)
                     /locus_tag="O2701_001919"
     CDS             complement(14275..15312)
                     /locus_tag="O2701_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCZ4293802.1"
                     /translation="MKTRCYDGKKWQYEFKYEGKRYRKKGFRTKREANSAGLDKLNEL
                     RSGFNIDNYITLEEYFENWIKTYKQPVVKENTYRHYRNALQHIQKHKIGKMELSKINR
                     QVYQKFINDYSKEHAKETIRKTNGAIRSALDDALYDGLIFKNPAYKVNYKAGKPTKSE
                     QEKFISVTEYEILKDHVRKKRTRSSLALFIMICTGCRVSGARNIKIEHINQVKNTIFI
                     DERKTDTSPRYISIAKSDMKHIMDVISTFAISYDGYIFKEAGSIINLHAINNALKSAC
                     RVNNISIITSHALRHTHCSYLLAKGVSIHYISKRLGHKNIAITTSVYSHLLEEKFNEE
                     DKKTTKILESM"
BASE COUNT         5615 a         2076 c         2661 g         5173 t
ORIGIN      
        1 cttaattaaa aggttatgac ttgaaatttt gaccaaattt gattattata aatgtatgtt
       61 agcactcttt aatgttaagt gctaaacttt aggtttttta aggaggaaca atcatgctaa
      121 aaccaattgg aaatcgtgtg attattgaga aaaaagaaca agaacaaaca actaaaagtg
      181 gtattgtttt aactgatagt gctaaagaaa aatcaaacga aggcgttatc gttgcagtag
      241 gaactggacg cctattaaat gatggtacaa gagtgactcc tgaagtgaaa gaaggggacc
      301 gtgtcgtgtt ccaacaatat gctggtacag aagttaaacg agataatgaa acatatctgg
      361 tattaaatga agaagatatt ttagcagtta ttgaataata cagaacttaa ttcataaata
      421 aattaaatag aacgaaaatg aaacacaact aaacaaatgg aggtttatca tttatggtta
      481 aacaattgaa attctctgaa gatgcacgtc aagcaatgtt acgtggtgtt gaccaacttg
      541 caaatgcagt taaagtaacg attggtccta aaggacgtaa tgttgtatta gataaagagt
      601 ttacagcacc tttaattacg aatgatggtg tgacgattgc taaagaaatc gaattagaag
      661 atccatatga aaatatgggg gctaaactag ttcaagaagt cgcaaataag acaaatgaaa
      721 ttgctggtga cggtacgaca actgcaacag tattagctca agcaatgatt caagaaggct
      781 tgaaaaatgt tacaagtggt gcgaacccag ttggtttacg acaaggtatc gacaaagcag
      841 ttaaagttgc tgttgaagcg ttacatgaaa attctcaaaa agttgaaaat aaaaatgaaa
      901 ttgcgcaagt aggtgcgatt tcagcagcag atgaagaaat tggacgttat atttctgaag
      961 ctatggaaaa agtaggtaac gatggtgtca ttacaattga agaatcaaat ggactaaaca
     1021 ctgaactaga agtggttgaa ggtatgcaat ttgatcgtgg ttatcaatca ccgtatatgg
     1081 ttactgattc agataaaatg gttgctgaat tagaacgccc atacatttta gtaacagata
     1141 agaaaatctc gtctttccaa gatatcttac ctttattaga acaagtggtt caatctaatc
     1201 gtccaatctt aattgtagct gatgaagttg aaggcgatgc attaacaaat atcgtgctaa
     1261 accgtatgcg tggcacattt acagctgttg cagtaaaagc acctggtttt ggtgatcgta
     1321 gaaaagcgat gcttgaagat ttagctattt taactggtgc gcaagtgatt actgatgatt
     1381 taggcttaga tttaaaagat gcatcaattg atatgttagg tactgcaagt aaagtagaag
     1441 taactaaaga taataccact gttgttgatg gtgacggtga cgaaaacagc attgatgcac
     1501 gtgttagcca attgaaatct caaattgaag aaactgaatc tgactttgat cgtgaaaaat
     1561 tacaagagcg cttagctaaa ttagcaggtg gtgttgcagt tatcaaagta ggtgcagcaa
     1621 gtgaaacaga gcttaaagaa cgtaaattac gtattgaaga tgcattaaat tctacacgtg
     1681 cagcagttga agaaggtatt gttgcaggtg gtggtactgc attagtaaat gtttaccaaa
     1741 aagtaagtga aattgaagct gaaggtgaca ttgaaacagg tgtaaatatt gtacttaaag
     1801 cattaactgc accagttcgt caaattgctg aaaatgcagg attagaaggt tctgttattg
     1861 tagaacgttt gaaaaacgca gagccgggtg ttggttttaa cgctgctaca aacgagtggg
     1921 ttaatatgtt agaagaaggt atcgttgatc caactaaagt aacacgctca gcattacaac
     1981 atgctgcaag tgttgcagca atgttcttaa cgactgaagc ggttgtagca tcaattccag
     2041 aaaaaaataa tgaccaacct aacatgggtg gcatgccagg aatgatgtaa aactgtttat
     2101 agttttgact aactagaaaa ttacattatg cacgagtatg attcattcta agtataaatt
     2161 agaatgcacg caaatatatt ttaattacga gacgtcttcc atttggtgac taaacttatg
     2221 ggaggcgtct tttttgtatg aatttgtgat tttagcatag atattcatag tggtattgat
     2281 atctttatgc cgtaatcgtt cttgtatttc tttaatgtgt acacctgatt ctatgagcaa
     2341 tgcgcaatgt gtgtatctga atgagtgcgt agagatattt ttgagtatgc tggtacgtga
     2401 cataatggtt tgtatccaca ttgatagttt tttcattaca atggttatca ttatataatt
     2461 cgtttttcca attattttga acgtttatct tataatcacg caacaccttt attacatttg
     2521 ggtcaacgga aatcttaccg atagaacttt cagttttagg gggaagtatc tgatatttct
     2581 tcttgttatt atttggattg taatatgttt tagtaatact aatcgtgttg ttttcaaaat
     2641 caatatcaga ccacttcaat gctagtatct cgccagcacg acagccggta tatgcaagtg
     2701 tgctaaagac ttcaaagcta gttaacgggg cgatgatgaa ataaatactg atgatttctt
     2761 tttaataggt gatgaagatt ctgacgaatt gatgacagac gataatgtca atgatcatat
     2821 tataaagaat cacatagaaa tgattgttga cagattagcg accgataaag agttttatat
     2881 ttttgactcc cttatacaag gacttagtta tcaagatatt agtagtgcct tagattgttc
     2941 agaacaatct gtaatattat ggtatgaaac catattagat aaaattgtgg gggtgataaa
     3001 atgagtgagt taactgcaaa gcaagcacgt tttgtgaatg agtatattag aacactgaat
     3061 gtaacacaaa gtgccataaa agcaggatat aacgcaaata gcgcacatgt gacagcgtgt
     3121 aggttattga aaaagccgca catcaagcaa tatatacaag aacaaaaaga taagattata
     3181 gatgagaatg tattaaccgc aaaagagtta ttacatgtgc taacgaatgc ggcagtcggt
     3241 gatgaaacag aaacgaaaga agttgtagtc aagcgtggag aatataaaga aaatccacaa
     3301 agtggcaaag tacaattagt ctataatgaa catgttgaac tgatagaggt accaataaaa
     3361 cctagtgatc gtttaaaagc tcgtgatatg ttgggtaaat atcataaatt atttacagat
     3421 aagcatgata taaacggtaa tgtacctata ttcattaata ttggtgaatg ggacggaggt
     3481 gatgaggggt tagataaggc agtgaaagat gtatctaacg ataatcctaa tcatactgtg
     3541 attgtggatg atataccgtt agaagattat gagtcaatta ggtttttata aattgggtat
     3601 atattttatg gtgttgccag tatttattga tagtatcggt taagacgatg agaagaatga
     3661 gtgagattta aaagagttgt aaacgcaata tcctaatact gattttcata ttgatgattt
     3721 agggaggttt tcaggagtaa aagtatgata tttttttcaa attacatcaa catttcagat
     3781 tgattattgt aatatataat aaaatgattt aagaaaaagg gagaaaaatg gataatataa
     3841 ataaaaatga aaaatttcgt gttttgtaaa caattgctat tactatatca tttattttaa
     3901 attttgggtt gtttatttct ctagctattt ggtcaataaa ttcgatgggt aataaacaat
     3961 gcattagttt gaatttattg ctttcggatg atactttcgc agtaatttca gtaatgacaa
     4021 ctttgggaac tgctatttta ggaggtatag caccttcaat atatggtatt tttacatcgc
     4081 ctatagataa aaaaagtagt tttagggcta ttagcgattg ggagcaagaa caaagaaata
     4141 atgttaaaga tctgatgatg ctattaccat ttacattact tagtatttta attgtgattt
     4201 ttatttttca gtttgcatat tctaaactat ttaatacgat agctattata atatttatta
     4261 taatacttgt tgttatgttt ttttaatttt attaatctgt tataaaatta ctgtgaagat
     4321 atgcagatat ttttcaaaag aatagaaatg atttgtttac gctgagaaac gtcctgattt
     4381 gcagtgagaa atgattttgc gtatctattg tatgtaacaa taggtacata atatttttag
     4441 ggtgggttat atgagcataa ttacaagatt gtttaataac agtgattttg aaaaattaaa
     4501 tcaactatgt aaattatatg atgatctagg ttatccaaca aatgagaatg atttaaaaaa
     4561 gagactaaag aaaataacga atcatgatga ttacttccta ctgcttttga taaaagaaaa
     4621 taaaataatt ggtttaagtg gtatgtgtaa aatgatgttt tacgaaaaaa atgcagagta
     4681 tatgagaatc cttgcgtttg ttatacattc tgaatttagg aaaaaaggtt atggaaagag
     4741 attattagct gattctgaag aattttctaa acggttgaat tgtaaagcaa taacactaaa
     4801 tagtggtaat agaaatgaaa gactatctgc acataaacta tatagtgata atgggtatgt
     4861 tagcaataca tctgggttta ctaaacaact ataagtgtga gcattcgtat aatacgatgt
     4921 aggaacatat tttattgtaa atgttgcatg tatgcctcgt attatgactt atttaaaggg
     4981 agaaaaaagg gcataattct tcgattgttg gagaaataga ctagattttc gagattttcc
     5041 aacaataaat caatgttgga aaacagtttt tgagtgacat aattatgaca taatagaatt
     5101 gaaaaactaa aaataatagc gttgtctaaa aattaaaacg ctgatttaat aatctttaaa
     5161 gcgtgtatta aagtaagttt ttattagtgc ataatgccgg gaatgatgta aaactgtata
     5221 tagttttgac taattagaaa attacattat gaacgagtat gatgtattct aagtacaaat
     5281 tagaatgcac gcaaatatat tttaattacg agacgttttc catttggtga ctagacttat
     5341 gggaggcgtc ttttttatgt gctaatttgt aattgagatt cataatggta tttaaatcat
     5401 tatggtgcgg gagttatggt attgccataa tatgagagtg catccacttc tataagtaat
     5461 gcatattgcg agtgcaggaa tgaatgagtg cttgattaaa atccttatgg gtggttgaca
     5521 taattaaaag aaaccacatt taaaatttct taatcacaag cggttaacta ggtatagttt
     5581 agttttgagt aaatcttttt aaaggtgtac ttgtgcattt tacttaatta aagacataag
     5641 acatttaatc ggcctaaaat aaataaaaaa ctacctgttt aggtagtttt tttaatgtaa
     5701 tagattaaaa cactagttca tttcttatta aagatggata gttattttat agataaattt
     5761 gtcctttagt gtagcggtaa tttttaggac tttttggtgg tataaatgtt cttaataaag
     5821 ttaatagtcc tactttaccg caaagcataa cgaatataat aattatttta gtaataccat
     5881 gatattctgt ggtaaggttc atacttaacc ctactgttcc gaatgcagaa accacttcga
     5941 ataataactt gattaatgat atgttcggat taattatcga taatataaaa gtaatgatgc
     6001 tgataaatag aaatgagata ttaatggtaa caatagatag ttttatatgt ttgtcagata
     6061 tttctttatt gaatactgaa acattatttt ctttacgtat ataatttagt acaaaaataa
     6121 acgcaactgc aaaagtagtt attttaattc ctccagctgc actgagaggg gcaccaccaa
     6181 taaacataag tagcattaac attaatgcgg tagatttgtt aatgcttgct atatctatac
     6241 tgttaaaacc cgctgttcgt gttgttactg attggaaaaa agaatttccg attttttcaa
     6301 ctagtcccat atgttgcata gtattaaact gttctaataa aaagaatgta atagctccta
     6361 taattattag gatactagtt gtagttaaga ctaatttaga atgtaaagat aatttactca
     6421 attttttaca attaataaag tctatcacga caaaatgtcc aatacctcca aatattatga
     6481 gtattgagat tgtaataatg acaattggat cactagaata atctattaag ttattcttaa
     6541 aaagggcaaa tccagcatta ttaaaagctg atactgatgt gaataagctt aaaaataaac
     6601 ctttgcctat accaaatttt ggtataaaag ataaacacaa acaaatcata ccaattaatt
     6661 cagtgactaa actataaata gccaagtgtt taattagctt aataacacca ccaggttcgt
     6721 caatattcca tgtaaccata atcaagaatc tatttttcat tgatatcttt ctatttaaaa
     6781 atactagtgt caatagggtt acggtcacga tacccagacc acctatttga attaataata
     6841 gtattactat ctcaccaagt atattaaact gtgatcctat atcaactggg gacaagccag
     6901 taactgtaaa tgcacttgaa gctataaata gggcatctaa aaaagatatt ggctttttac
     6961 cagtaaaagg taaatacaat aaaagtgcac ctatgatagt tgtagagaag aaaagcatta
     7021 aataaaaata taaaggtttg tggactttgt tcattttaat ttcatactcc tttatattat
     7081 aaattaataa ttagataata tcataaatga aaatagaaat gttactgatg tgtattacta
     7141 acaggcgttg actaattctt ttaatatttt ataattgaaa atgaatatca attgaaaaac
     7201 aaaaggatga tattatgaaa agattaattg gaattttatt atgtaattta tttattttga
     7261 ctgcgtgttc agcatctgtc gataaaacaa gtaactcgac aaagactaca gactataaaa
     7321 ttgaaaatgg tgaaacactg aaagtaccag agaaacctaa aagagttgct gtattaactg
     7381 gattttatgt tggtgatttt ataaagttag gaatcaaacc aattgctgtt tcagatataa
     7441 ctaaagattc ttcaatttta aaaccttatt taaaaggggt tgattatatt ggagaaaatg
     7501 atgttgaaag agttgctaaa gcaaaaccag atttaattgt tgtagatgct atggataaaa
     7561 atataaaaaa ataccaaaaa atagcgccaa caatcccata tacatacaat aaatacaatc
     7621 ataaagaaat attaaaagaa ataggcaagt tgactaataa tgaagataaa gctaaaaaat
     7681 ggattgaaga gtgggacgat aaaactagaa aagataaaaa agaaattcaa agtaaaattg
     7741 gtcaagcaac agcatctgtg tttgaaccag atgaaaagca aatatacata tataactcta
     7801 catggggtcg tggtttggat attgttcatg atgcattcgg tatgccaatg acaaagcaat
     7861 ataaagataa attacaagaa gataaaaaag gctatgcttc catttcaaaa gaaaatatta
     7921 gtaaatatgc tggtgattat atatttttaa gtaaaccttc gtacggaaaa tttgattttg
     7981 aaaaaacaca tacatggcag aatattgaag ctgtaaaaaa aggacatgta atttcatata
     8041 aagcagaaga ttattggttc acagatccta ttacattaga acatttgaga agtaaattaa
     8101 aaaaagaaat tttaaataaa aaataataga aataagttgt aaaaattttc ttatgcattg
     8161 gtactaatgt ttttaaggag tgattaaatg aagcaactgg ttggaattcc cgaatcaatg
     8221 ttaattcctt tgatagctcg agcaaaagag tacgaaaacg aaaaaccaat aataaaagac
     8281 gcactatcaa aaaaaatatt tgatggttta gatgatatgt acaaaaatgt tacatgtgat
     8341 gacatgtctc aaattggaat tagtatacgt actgtgataa tagattgtgt tactaagagg
     8401 cttatcaagg ataataaaga tttaatcgtg gtcaatatag gttgtggctt agatacaagg
     8461 tttcaaagat ttaataaaga aaaaatatca tggatagatt tagatgtacc agaatcaata
     8521 gaaatacgaa aaacattttt taaagaaagt aatagttata agatgatatc taaatctatg
     8581 ctagattaca gttggattga tgatgtcaaa aattataaat tttttaatag taagtcaaat
     8641 atattgttta ttattgaagg tgtattgatg tattttgatg agagtgtaat gactcaatta
     8701 ttggacacta ttatcaaaaa gatgggagat cataatttga catttgcgat tgaattttgc
     8761 tcaaaaacaa ttgcgaataa tacaaagaga catcaatcgg tatcaaagtt atcctcacaa
     8821 cctgttttta aatatggtta caatgattta aaaaaattga atgaaatttt accaaataca
     8881 ataagagtaa tacatgaata taattatttt gactattata agaatcgatg gggcttattt
     8941 ggatattgta gatttatacc ttatttaaaa aaaaggttaa acaataaaat agtacttatg
     9001 aaatataaag ttccaaagag acagagacat taagttctta gacaatgtaa aaaagctgat
     9061 ttctataaat tatttgatag aaatcagctt ttttcaaatg tatttgataa tttacagctc
     9121 gttgagctgc tgttttctag ggatttatgt cccattcctt ttacgagaag gtttttacga
     9181 gttttatgta actattagaa gctatctttt tatatgacca ttagtcaata tatgtatgac
     9241 aaatcagcac aataatttca gttttacatc acttaatcaa tgaaaatgtg cttgttgata
     9301 tttgaatatt tacattcaaa caatattcat cgctatttct atcaacttaa aattcgcaat
     9361 aaaaaactgc cagcacgcga atagggcagc tgacagcatc gttcttattt ttcttttaaa
     9421 tattgaatgg aagcctcttt aaaaatatca atatatttca gatacatatc tttttcaata
     9481 tattcatcga tttgatgtgc cattaatgga ttacctggtc caaaaatggc taaatcaaca
     9541 ttgtccttat tatctcctaa gaaactagag gcatctgttg cacctacaag cgctgaaaca
     9601 aatatttcgt cttgttctac ataactagaa gctacatctt taatcgtagt aattaatttg
     9661 ctatttttat cgcttgttac aggtcggtga ttgcttggaa tatcgagtga aagcttattg
     9721 ctatccacat cattaatgat attttggaaa aacgattcta taaagtcgtt atcatactca
     9781 ggaactggtc ttacgttaaa ttcaagtgaa gcttcatctg gtacagagtt aaattgtttg
     9841 ccgccattta taatcgaaca tacagctgta agaccagatg cataatttgc atcctcttca
     9901 gaaatttctt ttccaatcaa tgatttgaac ataggcgcaa catctaattc atgtttagta
     9961 tcttgttgtt taagctctga atatttttct ttaaatagat tataaaattc aagcagtgta
    10021 tcaattgcat tgtcaccaat aaatggaact gagctatgga cagctttacc agttgcagtt
    10081 actttacatg acatagaccc cttatgtgca taataaattc cagatccagt tggttcagca
    10141 ataattaagc tatcgacatc gtctaaatag cctttatcag ctaataattt ggcaccttct
    10201 tgttctttct cttcgccagc agtagccagt aatctaatcg ttccatgagg caattcattt
    10261 tgttctttta attcgattag agatacgacc aaagccatta aaccgccttt catatctgta
    10321 gtgcctcggc catataattt gccatctttt tctgtcagtt gaaaaggggg atatgaccaa
    10381 ttatcttgat ttcctgcatc aacaacatcc atatgaccac tcaatgcgag tataggtgag
    10441 ccgttaccga tttctgcaac gatattggcg cggtgttcat taactttcaa aatttcagat
    10501 ttaatatcgt acttgtcgaa taaatctgtt aaataattac aaacgtctat ttcattatta
    10561 ttttcagttt gtagttcaac aatatctgct agtaattgaa ttttttcttt ttcactaaaa
    10621 gttgtcatta agctcacacc tttcaaagta gtatatatat ttatataaac ataattttta
    10681 ttcttaaaca taatttaaaa tgcagaaagt agtgtgttat ttgatttcgt tctatgaaat
    10741 taactgtcta gtatcaacga tcttattaac gctgatatcg gatatgctta attttatagt
    10801 aaattgtatg agaaagatgc aggatattat ttagctaata attatatata aatttcaaat
    10861 cgatggttat taactactaa ataaaaatat ttgctaaatt aatgactttg tacacacatt
    10921 atttttaaag aggataaagt atttaataat attaacaaaa tcatttaata aatagttaaa
    10981 tatatattct ctgtttttgt gatattattc acatgtcgat acatatcaac aaatatcaat
    11041 catacgaaag aaggttataa caatgaaaaa taaaaaacgt gttttaatag cgtcatcatt
    11101 atcatgtgca attttattgt tatcagcagc aacgactcaa gcaaattcag ctcataaaga
    11161 ctctcaagac caaaataaga aagaacatgt tgataagtct caacaaaaag acaaacgtaa
    11221 tgttactaat aaagataaaa attcaacagc accggatgat attgggaaaa acggtaaaat
    11281 cacaaaacga actgaaacag tatatgatga gaaaacaaat atactccaaa atttacaatt
    11341 cgactttatc gatgatccaa cttatgacaa gaatgtatta cttgttaaaa aacaaggctc
    11401 aattcattca aatttaaagt ttgaatctca taaagaagaa aaaaattcaa attggttaaa
    11461 gtatccaagt gagtaccatg tagattttca agtaaaaaga aatcgtaaaa ctgaaatatt
    11521 agaccaattg ccgaaaaata aaatttcaac tgcaaaagta gacagtacat tttcatatag
    11581 ctcaggtggt aaattcgatt caacaaaagg tattggacga acttcatcaa atagctactc
    11641 caaaacgatt agttataatc agcaaaatta tgacacaatt gccagcggta aaaataataa
    11701 ctggcatgta cactggtcag ttattgcgaa tgacttgaag tatggtggag aagtgaaaaa
    11761 tagaaatgat gaattattat tctatagaaa tacgagaatt gctactgtag aaaaccctga
    11821 actaagcttt gcttcaaaat atagataccc agcattagta agaagtggct ttaatccaga
    11881 atttttaact tatttatcta atgaaaagtc aaatgagaaa acgcaatttg aagtaacata
    11941 cacacgaaat caagatattt tgaaaaacag acctggaata cattatgcac ctccaatttt
    12001 agaaaaaaat aaagatggtc aaagattaat tgtcacttat gaagttgatt ggaaaaataa
    12061 aacagttaaa gtcgttgata aatattctga tgacaataaa ccttataaag aaggataata
    12121 ttgaaagggc ggattactaa tgattaaaca actatgtaaa aatatcacaa tttgtacgtt
    12181 agcactatcg actactttca ctgtattacc agctacttca tttgcaaaga ttaattctga
    12241 aatcaaacaa gtttctgaga agaatcttga tggtgatact aaaatgtata cacgtacagc
    12301 tacaacaagt gatagtcaaa aaaatattac tcaaagctta caatttaatt tcttaactga
    12361 acctaattat gataaagaaa cagtatttat taaagcaaaa ggtacaattg gtagtggttt
    12421 gagaatttta gacccaaatg gttattggaa tagtacatta agatggcctg gatcttattc
    12481 agtttcaatt caaaatgttg atgacaacaa caatacaaat gtgactgact ttgcaccaaa
    12541 aaatcaggat gaatcaagag aagttaaata tacgtatggt tataaaacag gtggagattt
    12601 ttcgattaat cgtggaggct taactggaaa tattacaaaa gagagtaatt attcagagac
    12661 gattagttat caacaaccat catatcgtac attacttgat caatctacgt cacataaagg
    12721 tgtaggttgg aaagtagaag cacatttgat aaataatatg ggacatgacc atacgagaca
    12781 attaactaat gatagtgata atagaactaa aagtgaaatt ttttctttaa cacgaaatgg
    12841 aaatttatgg gcgaaagata atttcacacc taaagacaaa atgcctgtaa ctgtgtctga
    12901 agggtttaat ccagaatttt tagctgttat gtcacatgat aaaaaagaca aaggtaaatc
    12961 acaatttgtt gttcattata aaagatcaat ggatgagttt aaaatagatt ggaatcgcca
    13021 tggtttctgg ggctattggt ctggtgaaaa ccatgtagat aaaaaagaag aaaaattatc
    13081 agcattatat gaagttgatt ggaagacaca taatgtgaag tttgtaaaag tacttaatga
    13141 taatgaaaag aaataagtaa taaaagttgc ctgctacata gaatgtagta ggtaactttt
    13201 atttatattt gaatagatag atttattatg acgtgcagtg tatgaatctt gtttgagtgt
    13261 agagtaaaga cttgtattaa tgaaaaatta aagttgttaa gatgatttta ttaaaatgta
    13321 agtcaattca aattttaaca atataactcg cttcgtcctt ttagaacgaa gcaagttatt
    13381 agttagttga gcactattta ctataggctt tgattgggta atgatctgaa aaatcattgt
    13441 aaacgtagta atatgggaac gcatatacat cccatggctt aggtttttca gtcacaactt
    13501 cattgactaa ttgttttggt tgtttatgat ctttatctgt aaatatatag tctaaatgtt
    13561 ctggtttacc attagggtaa ttatatttcg caattgaatt tgattgaggg tcccatgtgc
    13621 tattatgacc tgcatataga acatcattta cattcaagtt tttaagcata tctttgaact
    13681 ctggagtgcc tttattgaca ttaaggtcgc cacctatata taccgtttca tctttaggga
    13741 tatttttctt tttaacaaag tcactgattt ctttcatttg ttcagctcta atttttcgat
    13801 catgtccagc accacaacgt gaatcttcag attgtgtatg tgtaccgata acgtgaacgt
    13861 tcttaccatt tttctctatt tttgtataaa caaagccttt gttgctatca ttatcgaatc
    13921 cacaaccgct tttgaaaaca tgctggattt tttctttaat aggatattta cttacaatcg
    13981 ctacgccacc atcttctgca acagttgatg agtagctacc ttcagttttg tcccaccctg
    14041 attgagaacg gccgagtaca ggtgtttgat aaggatattc ttttttcaca ttacttaata
    14101 atttgtctga tgcaccatta tcaaatgctt cattgaatat tacgacatca ttatttttaa
    14161 tataagaaga ttgtccgatt aaatcagcgc gtttatattg cccccagttt ggatacatag
    14221 aaaccttgta acaacagtat ttattgggtt tggagtccct aatgggtccc taaattacat
    14281 actttctaaa attttagttg tttttttgtc ctcttcatta aatttttctt ctaacaaatg
    14341 agaatacacg gatgtagtta ttgctatatt tttatgacct aatcttttag aaatgtaatg
    14401 tatagataca ccttttgcta gtaaataaga acaatgagtg tgtcttaatg cgtgcgatgt
    14461 aataattgat atattattga ctctacaggc tgatttcaaa gcattattga tagcatgaag
    14521 gttaattata gatccggctt ctttgaaaat gtaaccatca tagctaattg caaatgtact
    14581 tatgacgtcc ataatgtgtt tcatatcaga tttagcgata ctgatatatc taggggaagt
    14641 atcggttttt cgctcgtcaa taaatatagt gtttttcact tggttgatat gctcaatctt
    14701 tatatttctt gcaccactga cacgacaacc cgtacaaatc attatgaata gcgctaatga
    14761 tgaacgagtt ctcttctttc tgacgtgatc ttttagtatt tcatattcag ttaccgagat
    14821 gaatttttct tgttctgact tcgtaggttt tccggcttta taattaactt tataagcggg
    14881 atttttaaaa ataagcccat catataatgc gtcatctaaa gctgaccgaa tagcaccgtt
    14941 tgtttttctt atagtttctt ttgcgtgttc ttttgaataa tcgtttatga atttctgata
    15001 aacttgtcta tttatctttg ataactccat tttacctatt ttatgttttt gtatatgttg
    15061 taatgcattt ctataatgac ggtaggtatt ttctttaaca acaggttgtt tatacgtttt
    15121 aatccaattt tcgaagtatt cttcaagagt tatatagtta tctatattaa aaccacttct
    15181 taactcattt aacttgtcta gtccagcaga attagcttca cgctttgttc taaaaccttt
    15241 cttacggtat ctttttcctt catacttaaa ttcatattgc cattttttac catcgtaaca
    15301 acgtgttttc atgcgttccc tcctcaaaat tggcaaaaaa taataagggt aggcgggcta
    15361 cccatgaaaa ttgtataaaa aaagacgcct gtataaaata cagacgccac ttatagttat
    15421 aagattacat ggttaattac caaaaatggt aacgaatata tacgtgtttt aaaggataaa
    15481 cctttaatat attaaaatta tatcatctta tatcaggaat ctgca
//
LOCUS       DALAGD010000073        15103 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.074, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000073 DALAGD010000000
VERSION     DALAGD010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15103
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.074"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..118
                     /locus_tag="O2701_001920"
     CDS             <1..118
                     /locus_tag="O2701_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HCZ4293803.1"
                     /translation="TMINNVTAFTANIYYKSIIKITITVKVGTNVQTKTIYV"
     gene            215..361
                     /locus_tag="O2701_001921"
     CDS             215..361
                     /locus_tag="O2701_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293804.1"
                     /translation="MMLTAIYFSIFTFYISQYSLKLKTLYNIETDYNYKIVLLLKEGK
                     THES"
     gene            351..875
                     /locus_tag="O2701_001922"
     CDS             351..875
                     /locus_tag="O2701_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HCZ4293805.1"
                     /translation="MNHDKSPIILIGFMGTGKSTIGKYVADEQNLSFIDIDSYIEEKY
                     KLTIPEIFCKHGEQYFRNLEFTCLQECINTADIIATGGGIIESEEAFNFLKNQKNIIW
                     LDCNIDIIYSRINDDPHRPNANNKTIKQLNDLYCSRNLRYNEIAFKKFDSHLLSISEI
                     YYELLNLIKASDQY"
     regulatory      862..959
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            1034..2125
                     /gene="gcvT"
                     /locus_tag="O2701_001923"
     CDS             1034..2125
                     /gene="gcvT"
                     /locus_tag="O2701_001923"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCZ4293806.1"
                     /translation="MSSDLKQTPLYQNYVDRGAKIVEFGGWAMPVQFSSIKEEHNAVR
                     YEIGLFDVSHMGEIEVTGKDASQFVQYLLSNDTDNLTTSKALYTALCNEEGGIIDDLV
                     IYKLADDNYLLVVNAANTEKDFNWILKHKEKFDVEVQNVSNQYGQLAIQGPKARDLIN
                     QLVDEDVTEMKMFEFKQGVKLFGANVILSQSGYTGEDGFEIYCNIDDTEKIWDGLLEY
                     NVMPCGLGARDTLRLEAGLPLHGQDLTESITPYEGGIAFASKPLIDADFIGKSVLKDQ
                     KENGAPRRTVGLELLEKGIARTGYEVMDLDGNIIGEVTSGTQSPSSGKSIALAMIKRD
                     EFEMGRELLVQVRKRQLKAKIVKKNQIDK"
     gene            2145..3491
                     /gene="gcvPA"
                     /locus_tag="O2701_001924"
     CDS             2145..3491
                     /gene="gcvPA"
                     /locus_tag="O2701_001924"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HCZ4293807.1"
                     /translation="MSHRYIPLTEKDKQEMLQTIGAKSIGELFGDVPSDILLNRDLNI
                     AEGEAETTLLRRLNRIASKNITKETHTSFLGAGVYDHYAPSVVDAMISRSEFYTAYTP
                     YQPEISQGELQAIFEFQTLICELTDMDVANSSMYDGMTSFAEACILAFSQTKKNKIVV
                     SKGLHYQALQVLHTYAKTRKEFEVVEIDLDGTVTDLKKLEAAVDDETAAVAVQYPNFY
                     GSIEDLEKIHSFIEDKKALFIVYANPLALGLLTPPGSFGADIVVGDTQPFGIPAQFGG
                     PHCGYFATTKKLMRKVPGRLVGQTQDDEGNRGFVLTLQAREQHIRRDKATSNICSNQA
                     LNALASSIAMSALGKQGIYDIAVQNIEHANYAKQQFIKKGFEVLDGTSFNEFVVKFDK
                     PIQQVNEELVKYNIIGGFDLGVVSDDFKNHMLIAVTELRTKDEIDTFVEKAGELND"
     gene            3484..4956
                     /gene="gcvPB"
                     /locus_tag="O2701_001925"
     CDS             3484..4956
                     /gene="gcvPB"
                     /locus_tag="O2701_001925"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HCZ4293808.1"
                     /translation="MTSKSSPLIFERSREGRYAYSLPKSDIKTNSVESLLDDKFIRKN
                     KAEFPEVAELDLVRHYTELSNKNFGVDNGFYPLGSCTMKYNPKINEKVARIPGFSESH
                     PLQDEDQVQGSLEIIYSLQEELKEITGMDEVTLQPAAGAHGEWTALMIFKAYHENNGE
                     GHRDEVIVPDSAHGTNPASASFAGFKSVTVKSNERGEVDIDDLKRVVNENTAAIMLTN
                     PNTLGIFEKNIMEIREIVHNAGGLLYYDGANLNAIMDKVRPGDMGFDAVHLNLHKTFT
                     GPHGGGGPGSGPVGVVKELASYLPKPMVIKDGDKFKYDNDIKNSIGRVKPFYGNFGIY
                     LRAYTYIRTMGATGLKEVSEAAVLNANYIKARLSKHFEIPYKQYCKHEFVLSGVRQKE
                     FGVRTLDMAKRLLDFGVHPPTIYFPLNVEEGMMIEPTETESKETLDYFIDTLISIAEE
                     AKNDPDKVLEAPHTTVIDRLDEATAARKPILKFENLKQEK"
     gene            complement(5321..5707)
                     /locus_tag="O2701_001926"
     CDS             complement(5321..5707)
                     /locus_tag="O2701_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCZ4293809.1"
                     /translation="MSASLYIAIILVIAIIAYMIVQQILNKRAVKELDQNEFHNGIRK
                     AQVIDVREKVDYDYGHINGSRNIPMTMFRQRFQGLRKDQPVYLCDANGIASYRAARIL
                     KKNGYTDIYMLKGGYKKWTGKIKSKK"
     gene            5865..6695
                     /locus_tag="O2701_001927"
     CDS             5865..6695
                     /locus_tag="O2701_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCZ4293810.1"
                     /translation="MTETWNFINTGSKDPYYNMAMDEALLNFVSRGEIDPVIRFYTWN
                     PATLSIGYFQRLQKEIDIDKVKEKGFGLVRRQTGGRGVLHDKELTYSVIVPESHPNMP
                     STVTEAYRVISQGLLEGFKNLGFDTYFAVPKTPEERQKLKQPRSSVCFDAPSWYELVV
                     EGRKIAGSAQTRQKGVILQHGSILQDIDIDELFDMFIYKNERLKLKMKEAFVEKAVAI
                     NDISDEHITISQMEEAFEKGFKKGLNIEFKPLELTEAQLAEVEELTEKYRSDEWMFRK
                     "
     gene            complement(6759..6977)
                     /locus_tag="O2701_001928"
     CDS             complement(6759..6977)
                     /locus_tag="O2701_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293811.1"
                     /translation="MRNIIFYLVLIIAAIGLVMNLDAFIFSIVRMLISFAVIAGIIYL
                     IYYFFILTEDQRKYRKAMRKYKRNQRRK"
     gene            complement(6991..7572)
                     /locus_tag="O2701_001929"
     CDS             complement(6991..7572)
                     /locus_tag="O2701_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293812.1"
                     /translation="MKKLVSIVGATLLLAGCGSQNLAPLEEKTTDLREDNHQLKLDIQ
                     ELNQQISDSKSKIKGLEKDKENSKKTASNNTKIKLMNVTSTYYDKVAKALKSYNDIEK
                     DVSKNKGDKNVQSKLNQISNDIQSAHTSYKDAIDGLSLSDDDKKTSKNIDKLNSDLNH
                     AFDDIKNGYQNKDKKQLTKGQQALSKLNLNAKS"
     gene            7677..8738
                     /locus_tag="O2701_001930"
     CDS             7677..8738
                     /locus_tag="O2701_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCZ4293813.1"
                     /translation="MSRITQVHRILEQKHLDAIIILSDYNRRYLSGFTGTSGALIISK
                     DKQYLITDFRYIDQATKQAPNYEIINRKSTIIGEIKELLHQENFENVGFEGHHVSYDT
                     YLELNKSRISLISISNTVDKIRDVKDADEIALIQKAANIVDETYEYILTVVKAGMTEK
                     ELKAILESKMLELGADGPSFDTIVASGHRGALPHGVASDKIIEKGDMITLDFGAYYNG
                     YCSDITRTFAIGEPDPKLKEIYQIVLESQMKAINEIRPGMTGAEADAISRNYLESKGY
                     GKEFGHSLGHGIGLEIHEGPMLARTIQDKLQVNNCVTVEPGVYIEGLGGIRIEDDILI
                     TENGCQVFTKCTKDLIVLT"
     gene            8764..9321
                     /gene="efp"
                     /locus_tag="O2701_001931"
     CDS             8764..9321
                     /gene="efp"
                     /locus_tag="O2701_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCZ4293814.1"
                     /translation="MISVNDFKTGLTISVDNAIWKVIDFQHVKPGKGSAFVRSKLRNL
                     RTGAIQEKTFRAGEKVEPAMIENRRMQYLYADGDNHVFMDNESFEQTELSSDYLKEEL
                     NYLKEGMEVQIQTYEGETIGVELPKTVELTVTETEPGIKGDTATGATKSATVETGYTL
                     NVPLFVNEGDVLIINTGDGSYISRG"
     gene            9795..10259
                     /gene="accB"
                     /locus_tag="O2701_001932"
     CDS             9795..10259
                     /gene="accB"
                     /locus_tag="O2701_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCZ4293815.1"
                     /translation="MNFKEIKELIEILDKSTLTEINIEDTKGKVTLKKEKETEIITPQ
                     ISQMPVEAAAMPMPQAQSTDSNKTEAPKPTSDNHKTINAPMVGTFYKSPSPDEEAYVQ
                     VGDTVSNETTVCILEAMKLFNEIQAEISGEIVEILVEDGQMVEYGQPLFKVK"
     gene            10259..11614
                     /gene="accC"
                     /locus_tag="O2701_001933"
     CDS             10259..11614
                     /gene="accC"
                     /locus_tag="O2701_001933"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCZ4293816.1"
                     /translation="MKKVLIANRGEIAVRIIRACRDLGIQTVAIYSEGDKDALHTQIA
                     DEAYCVGPTLSKDSYLNIPNILSIATSTGCDGVHPGYGFLAENADFAELCEACQLKFI
                     GPSYQSIQKMGIKDVAKAEMIKANVPVVPGSDGLMKDVSEAKKIAKKIGYPVIIKATA
                     GGGGKGIRVARDEKELETGFRMTEQEAQTAFGNGGLYMEKFIENFRHIEIQIVGDSYG
                     NVIHLGERDCTIQRRMQKLVEEAPSPILDDETRREMGNAAVRAAKAVNYENAGTIEFI
                     YDLNDNKFYFMEMNTRIQVEHPVTEMVTGIDLVKLQLQVAMGDVLPYKQEDIKLTGHA
                     IEFRINAENPYKNFMPSPGKIEQYLAPGGYGVRIESACYTNYTIPPYYDSMVAKLIIH
                     EPTRDEAIMAGIRALSEFVVLGIDTTIPFHIKLLNNDIFRSGKFNTNFLEQNSIMNDE
                     G"
     gene            11629..11991
                     /locus_tag="O2701_001934"
     CDS             11629..11991
                     /locus_tag="O2701_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCZ4293817.1"
                     /translation="MVKVTDYSNSKLGKVEIAPEVLSVIASIATSEVEGITGHFAELK
                     ETNLEKVSRKNLSRDLKIESKEDGIYIDVYCALKHGVNISKTANKIQTSIFNSISNMT
                     AIEPKQINIHITQIVIEK"
     gene            12051..12440
                     /gene="nusB"
                     /locus_tag="O2701_001935"
     CDS             12051..12440
                     /gene="nusB"
                     /locus_tag="O2701_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCZ4293818.1"
                     /translation="MSRKESRVQAFQTLFQLEMKDSDLTINEAISFIKDDNPDLDFEF
                     IHWLVSGVKGHEPVLDETISPYLKDWTIARLLKTDRIILRMATYEILHSDTPAKVVMN
                     EAVELTKQFSDDDHYKFINGVLSNIKK"
     gene            12457..13794
                     /locus_tag="O2701_001936"
     CDS             12457..13794
                     /locus_tag="O2701_001936"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCZ4293819.1"
                     /translation="MSDYLSVSALTKYIKYKFDQDPHLQSVLIKGELSNFKKHSSGHL
                     YFNVKDKESVISAMMFKGSASKLNFEPKEGDEVLLEARVSVFERRGNYQIYVNKMQLD
                     GIGNLYQKLEALKKKLTEEGCFDKANKKSIPKFPKKIAVLTASTGAAIRDIHSTINSR
                     FPLAEQIQISTLVQGEKAKDDIIEKIEYADSLGVDTIIVGRGGGSIEDLWNFNEEAVV
                     RAIYNCKTPIISAVGHETDFTLSDFAADIRAATPTQAAVIATPDQYELLQQIQQYQFT
                     LTRFIKKHLEQQRKHVEHLSSYYKFKQPTLLYDQQIQRRDDLEKRLKQQIQATFEQQR
                     HRLMLLQQRYNLKALLSSVNQEQQNNLQLTNQLVKLLNSKILSYKNDLKNKVENLNNL
                     SPTNTMLRGYAIVNKKDEVITSTKDLTENDQLTLTMKDGLVDAKVTKVRCNND"
     gene            13787..14017
                     /locus_tag="O2701_001937"
     CDS             13787..14017
                     /locus_tag="O2701_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCZ4293820.1"
                     /translation="MTKETQSFEEMMQELEQIVQKLDNETVSLEESLDLYQRGMKLSA
                     ACDTTLKNAEKKVNDLIKEEAEDVKNDESTDE"
     gene            13995..14876
                     /locus_tag="O2701_001938"
     CDS             13995..14876
                     /locus_tag="O2701_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCZ4293821.1"
                     /translation="MTNLPMNKLIDEVNNELSVAINKSVMDTQLEESMLYSLNAGGKR
                     IRPVLLLLTLDSLNTEYELGMKSAIALEMIHTYSLIHDDLPAMDNDDYRRGKLTNHKV
                     YGEWTAILAGDALLTKAFELISSDDRLTDEVKIKVLQRLSIASGHVGMVGGQMLDMQS
                     EGQPIDLETLEMIHKTKTGALLTFAVMSAADIANVDDTTKEHLESYSYHLGMMFQIKD
                     DLLDCYGDEAKLGKKVGSDLENNKSTYVSLLGKDGAEDKLTYHRDAAVDELTQIDEQF
                     NTKHLLEIVDLFYSRDH"
BASE COUNT         5542 a         2049 c         2776 g         4736 t
ORIGIN      
        1 aacaatgatt aataatgtta cagcatttac tgcaaatatc tactataaat ccattattaa
       61 aataacgata acagttaaag tcggtacaaa tgtgcagact aaaactattt atgtataact
      121 tactagtatt taaatgagta ctattaaaaa ggatgtgaaa taatttatta attttgattt
      181 atataaagaa aaaggatatg ttacgccgtt cttattgatg ctaacagcaa tttatttttc
      241 cattttcact ttttatatca gtcaatatag tttaaaatta aagacattat ataatattga
      301 aactgactat aactataaga ttgttttgtt attgaaagaa gggaaaacgc atgaatcatg
      361 ataaatcacc aataatttta attggtttca tgggtaccgg taaatctacg attggtaaat
      421 acgttgcaga tgagcaaaat ttatcattta ttgatataga ttcatatatc gaagagaagt
      481 ataagttaac aataccagaa atattttgta aacatggtga acaatatttc aggaatttag
      541 agttcacatg tttgcaagaa tgtattaaca ctgcagatat aattgctact ggtggtggta
      601 ttattgagag tgaagaggca tttaattttt tgaaaaatca aaaaaacatt atttggttag
      661 attgtaatat tgatattata tatagtcgaa tcaatgatga cccacatcga cctaatgcaa
      721 ataataagac aatcaagcag ttaaatgact tgtattgctc gcggaattta agatataatg
      781 aaatcgcatt caagaaattt gatagtcatt tgctatcaat ttcagaaata tattatgaat
      841 tgctaaattt aataaaagcg agtgatcagt attagagaga atagagcgtt aagactctat
      901 cgccgaaggt gcaagtaatt tattacgaaa ctctcaggca aaaggataat actgtaacgc
      961 gttcctgaat tggtgattta taaacagggt agcgattgct atcctgtttt tataatttta
     1021 agggggtatt tcaatgtcaa gtgatttaaa acaaacacct ttatatcaaa attatgttga
     1081 tagaggtgca aaaattgtgg aattcggagg atgggcgatg cctgttcaat tttcaagtat
     1141 taaagaggag cataatgctg ttcgatacga aattggcctg tttgatgtta gtcatatggg
     1201 tgaaattgaa gtaacaggta aagatgctag tcagtttgtg caatatttat tatcaaatga
     1261 tactgataat ttaactactt caaaagcatt atatactgct ttatgtaatg aagaaggcgg
     1321 tattattgat gatttagtaa tatataaatt agctgacgac aattatttat tagttgttaa
     1381 tgctgctaat actgaaaaag attttaattg gattttaaaa cacaaagaga aatttgatgt
     1441 tgaagtacaa aatgtatcaa accaatatgg tcaattagca atacaaggac caaaagctag
     1501 agatttaatt aatcaattag ttgatgaaga tgtaactgaa atgaaaatgt ttgaatttaa
     1561 acagggtgtc aaattatttg gagcaaacgt cattttatct cagtcaggtt acacaggtga
     1621 agatgggttt gaaatttatt gtaatatcga tgatactgaa aaaatttggg atggtttatt
     1681 agagtataat gttatgccat gtgggttagg cgctcgtgat acattaagat tagaggctgg
     1741 attgccatta catggacaag atttaactga atcaattaca ccatatgaag gtggtatcgc
     1801 ttttgcaagt aaaccattaa ttgatgctga ttttattggt aaatctgtat taaaagatca
     1861 aaaagaaaat ggtgcaccaa gaagaacagt gggattagaa ttacttgaaa aaggaattgc
     1921 aagaactggt tatgaagtta tggatttaga tggaaatatt attggagaag taacttcagg
     1981 aacacagtct ccatcatcag gaaaatcaat tgcacttgca atgataaaaa gagatgagtt
     2041 tgaaatgggt agagagttgc ttgttcaagt tcgtaagcgt caattaaaag cgaaaattgt
     2101 taagaaaaat caaattgata aataattaaa aaggggtgtg cattgtgagt catcgttata
     2161 tacctttaac tgaaaaagac aagcaagaaa tgttacaaac aattggtgca aaatctatag
     2221 gagaattatt cggtgatgta ccaagtgaca ttttattaaa tagagattta aatattgctg
     2281 aaggcgaagc ggaaacaacg ttacttagaa gattaaatcg cattgcaagc aagaatatca
     2341 ctaaagaaac gcatacatca tttttgggtg ctggtgttta tgatcattac gcgccatcag
     2401 ttgtggatgc tatgatttca agatcagaat tttacacagc atatacgcca tatcaaccgg
     2461 aaatttctca aggtgaatta caagccattt ttgagttcca aactttaatt tgtgaattaa
     2521 cagatatgga tgtagcaaat tcttctatgt atgatgggat gactagcttt gctgaagcat
     2581 gtatcttagc atttagtcaa acaaagaaaa ataaaattgt agtctcaaaa ggtttacatt
     2641 atcaagcatt acaagtactt catacttatg ctaagacgcg taaggaattc gaagtagtgg
     2701 aaatcgattt agatggcact gttacagatt tgaaaaaatt agaagcagca gtggatgatg
     2761 aaacagcagc tgttgcagta caatatccaa acttttacgg ttcaattgaa gatcttgaaa
     2821 agattcatag ctttattgaa gataaaaaag cattatttat cgtatatgca aatccattag
     2881 cattgggatt acttacacct ccaggttcat ttggtgcaga tattgttgta ggtgatactc
     2941 agccattcgg aattcctgct caattcggtg ggccacattg tggatatttt gcaacgacga
     3001 aaaagttaat gcgtaaagtg ccaggtagat tagtcggtca aacacaagat gatgaaggta
     3061 atcgtggctt tgtattaaca ttacaagcac gtgaacaaca tattcgacgc gataaagcga
     3121 catctaatat ttgttctaac caagcattaa atgctttagc gtcatcaatt gcaatgtcag
     3181 cacttggtaa acaaggtatc tatgatattg cagttcaaaa tatagaacat gctaactatg
     3241 ctaaacaaca atttattaaa aaaggttttg aagtattaga tggtacatct ttcaatgagt
     3301 ttgtagtcaa atttgacaaa ccaattcaac aagttaacga ggaattagtg aagtacaata
     3361 ttatcggtgg ttttgattta ggtgttgttt cagacgactt taaaaatcac atgttaatag
     3421 ctgtaactga attaagaaca aaagatgaaa tcgatacatt tgtagaaaag gctggtgagt
     3481 taaatgacta gtaaatcaag tccattaatt tttgaaagat ctcgtgaagg cagatatgca
     3541 tattcattac caaaaagtga tattaaaaca aattctgttg agtcattgtt agatgataaa
     3601 tttattcgta aaaacaaagc ggagtttcct gaagttgctg aactagactt agtacgtcat
     3661 tatactgaac tttcaaataa aaatttcggt gtagacaacg gattttatcc attgggttca
     3721 tgtacgatga agtataaccc taaaattaat gaaaaggttg ctagaatacc aggatttagt
     3781 gaatcacatc cattacaaga tgaagatcaa gttcaaggtt cattagaaat tatttatagt
     3841 ttgcaagaag aattaaaaga aattactggt atggatgagg tgacattaca accagctgct
     3901 ggcgcacatg gtgaatggac tgcattgatg atatttaaag cttaccatga gaataatggt
     3961 gaaggtcatc gtgatgaagt cattgtgcca gattctgcgc atggtacgaa tccagcctca
     4021 gcttcatttg caggatttaa atcagttact gtaaaatcaa acgaacgtgg cgaagttgat
     4081 attgatgact tgaaacgtgt tgtaaatgaa aatacagcag ctattatgtt aactaatcca
     4141 aacactttag gtattttcga aaaaaatatt atggaaatcc gtgaaatcgt ccataatgct
     4201 ggtggtctat tatattatga tggtgcgaat ttaaacgcta ttatggacaa agttcgccca
     4261 ggagatatgg gatttgatgc tgttcattta aacttgcata aaacatttac tggtccacat
     4321 ggtggtggcg gtcctggttc aggtccagtc ggtgtagtaa aagaactagc aagttactta
     4381 ccaaagccaa tggttattaa agatggcgac aaatttaaat atgataatga cattaaaaat
     4441 tctatcggac gtgtaaaacc attttatggt aactttggta tttacttaag agcttatacg
     4501 tatattcgaa ctatgggagc aactggactt aaagaggttt ctgaagcagc ggttcttaat
     4561 gcgaattata ttaaagcacg tttatctaaa cactttgaaa taccttataa acaatattgt
     4621 aaacacgagt ttgtgttaag tggtgtgcgt caaaaagaat ttggtgtacg tactttagac
     4681 atggctaagc gattattaga tttcggtgta catccaccaa caatatactt cccattaaat
     4741 gttgaagaag gtatgatgat tgaaccgact gagacagagt ctaaagaaac acttgattat
     4801 tttatcgata cattaattag tattgctgaa gaagctaaaa atgatcctga taaagtgcta
     4861 gaagcaccac atacaactgt gattgatcga ttagacgaag ctacagctgc tcgtaaacca
     4921 atattaaagt ttgaaaatct taaacaggaa aaataaagta ttaaacacat attccgagaa
     4981 ttattattct aactttgtat gaagatttaa ggataatggt ttcaaaatca attgaaaaag
     5041 acaatttcta tttaaacaag aaaactaaac cgaagtaata actcttaggg tttggtatta
     5101 ttctttcata gaaattgtct tttcattttt tagattgcgg taattgaatc gtattgaaaa
     5161 tgagctgaac tttctttatt atgctgaaac taagtttaat gatcagtgaa gctagagaaa
     5221 tatagtaaca gactttgtta aaaaattaaa atctgagtat tgttattgat gatcaattaa
     5281 cactcagatt ttattaaatc gtatattaaa tttacaaaaa ctatttttta gactttattt
     5341 ttccagtcca ttttttatag ccgcctttta acatatagat atctgtatat ccattctttt
     5401 tcaaaatacg agcggctcta tagctagcaa tcccattggc atcacataag tataccggtt
     5461 gatcttttct taatccttgg aatcgttgcc tgaacattgt cataggaata ttgcgagacc
     5521 cattaatgtg accgtagtca tagtcaactt tctctctaac atcgatgact tgagcttttc
     5581 taatcccatt atggaattca ttttgatcta attctttaac agctcgcttg ttaagaattt
     5641 gttgaacaat catataagca ataattgcta taactaaaat tattgcgatg tacaaactag
     5701 cactcatctt gcatcctccc taattaaacg ataatataat tataagactg ttaggtcaaa
     5761 ttatcaaaat atttcactaa agaattgcaa ttattattca tttagttaaa ttgattaatt
     5821 agcgaatcaa atattataca ttttgctgat aggagttctt gaatatgact gaaacttgga
     5881 attttattaa tacaggaagt aaagacccat attataatat ggcaatggat gaagcgttat
     5941 taaattttgt ctctagaggt gaaattgatc cagttatacg tttttacaca tggaatcctg
     6001 caacattatc aatagggtat ttccaaaggc tacaaaaaga aattgatatt gataaggtta
     6061 aagagaaagg ttttggtctc gtaaggcgtc aaactggtgg ccgtggggtg ctacatgata
     6121 aagagttaac ttacagtgtg attgtgcctg aatcgcatcc gaatatgcct tctacagtta
     6181 cagaagcgta cagagtgatt tcacaaggat tattagaagg atttaaaaac ttaggatttg
     6241 atacttattt tgcggtacct aaaacgcctg aagagcgtca aaaattaaaa caacctcgaa
     6301 gttcagtgtg ttttgatgca ccaagttggt atgagctagt tgttgaaggc cgtaagattg
     6361 ctgggagtgc tcaaacgaga caaaagggcg taatcctcca acacggttcg attttacaag
     6421 atattgatat tgatgaactg tttgatatgt ttatttataa aaatgaaaga ttaaaattaa
     6481 aaatgaaaga ggcattcgtt gaaaaagcag ttgccatcaa tgatatttca gatgaacaca
     6541 ttactattag tcaaatggaa gaagctttcg agaaaggttt taagaaaggt ttaaatatag
     6601 aatttaagcc acttgaatta acagaagctc aattggcaga agtggaagaa ttaactgaaa
     6661 aatatcgttc agatgaatgg atgtttagaa agtagttttt agaataaaaa atggaagcac
     6721 gtagactatt ttacctacaa gtgcttccgt ttttttatct attttcttct ttgatttctt
     6781 ttatacttac gcattgcttt gcgatatttg cgttggtctt cagttaagat gaagaaataa
     6841 taaatcagat aaataatacc agctattaca gcaaagctga ttaacattct gacgattgaa
     6901 aaaataaagg catctagatt cattactaat ccaatcgcag caataataag tacaagataa
     6961 aatattatat tacgcattaa aagactccta tcatgatttt gcatttaagt ttaattttga
     7021 caacgcttgt tgtccttttg taagttgttt tttatcttta ttttgatagc catttttaat
     7081 atcatcaaat gcatgattca aatcagagtt taatttatcg atatttttag acgttttttt
     7141 atcatcatca ctaagtgata aaccatcgat agcatctttg tatgaagtgt gagcactttg
     7201 aatatcatta gaaatttgat ttaatttcga ttgaacattc ttatcgcctt tgtttttact
     7261 tacatctttc tcaatatcgt tataggattt caaagcttta gcaactttgt cgtagtatgt
     7321 tgatgtaaca ttcatcaatt taattttcgt attattagat gcagtttttt tactgttttc
     7381 tttatccttt tcaagccctt taattttaga tttagaatca ctaatttgtt gattaagttc
     7441 ttgaatatct agtttgagtt gatgattatc ttctcttaaa tctgttgttt tttcttctaa
     7501 tggtgctaaa ttttgtgatc cacatccagc taacaataat gttgcgccaa caattgaaac
     7561 caattttttc attactatct ccctaaaatc aatatctatt attttttatc cttattatga
     7621 tattatttta atgtataaag ttgaaatgca caaacgtttc actaggaggt aataagatga
     7681 gcagaataac acaagtgcat cgtattttag aacaaaaaca tttagatgcg attatcattc
     7741 tttcggatta caatagaaga tatttatcag gttttactgg taccagtgga gcacttatca
     7801 tatctaaaga taaacaatat ttgataacag actttagata tattgatcaa gcaactaagc
     7861 aggcgccaaa ttatgaaatt attaatcgta aatctactat tattggtgag attaaagaat
     7921 tgctacacca agaaaatttt gaaaatgtag gttttgaggg gcatcatgta agttatgata
     7981 cataccttga attaaataaa agccgtatat cattaataag catttctaat actgtagata
     8041 aaattagaga cgtcaaagat gctgacgaaa ttgctttaat tcaaaaagca gctaatattg
     8101 ttgatgaaac atatgaatat attttaactg ttgtaaaagc aggcatgact gaaaaagaat
     8161 taaaggcaat attagaaagc aaaatgctag aattaggagc agatggacca tcattcgata
     8221 cgattgtagc atctggtcat agaggtgcat taccacatgg tgttgcaagt gataaaatta
     8281 ttgaaaaagg cgacatgatt acattagatt ttggcgcgta ttataacggc tattgttcag
     8341 atattactag aacatttgct attggagaac cagatcctaa actgaaagaa atatatcaaa
     8401 tagtacttga atctcaaatg aaagcaatta atgagattag acctggcatg actggtgcag
     8461 aagctgatgc catttcaaga aactatttag agtcaaaagg gtatggaaaa gaatttggac
     8521 attcactagg acatggtatt ggtttagaaa tccatgaagg gccaatgctg gctcgtacga
     8581 tacaagataa acttcaagtt aacaactgtg ttacagtaga acctggtgtt tatatagaag
     8641 gtttgggcgg tataagaata gaagatgata ttttaattac agaaaatggt tgtcaagtct
     8701 ttactaaatg cacaaaagac cttatagttt taacataagc gtgtaaaatg aggaggaaac
     8761 tgaatgattt cggttaatga ttttaaaaca ggtttaacaa tttctgttga taacgctatt
     8821 tggaaagtta tagacttcca acatgtaaag cctggtaaag gttcagcatt cgttcgttca
     8881 aaattacgta atttaagaac tggtgcaatt caagagaaaa cgtttagagc tggtgaaaaa
     8941 gttgaaccag caatgattga aaatcgtcgc atgcaatatt tatatgctga cggagataat
     9001 catgtattta tggataatga aagctttgaa caaacagaac tttcaagtga ttacttaaaa
     9061 gaagaattga attacttaaa agaaggtatg gaagtacaaa ttcaaacata cgaaggtgaa
     9121 actatcggtg ttgaattacc taaaactgtt gaattaacag taactgaaac agaacctggt
     9181 attaaaggtg atactgcaac tggtgccact aaatcggcaa ctgttgaaac tggttataca
     9241 ttaaatgtac ctttatttgt aaacgaaggt gacgttttaa ttatcaacac tggtgatgga
     9301 agctacattt caagaggata atctctaatt tgttaacaaa tagcttgtat tcactatact
     9361 gatttaacgt aagatattct aaataagtct cataaagcta ttgcctaaaa tgattatagg
     9421 ttatatgctg atatgaggct ttttattttt aaaataattt ttaaaatata aaacgaatcg
     9481 tctaattggt agtgaaaaaa catagacatg agtagaggta tatatctaca tatcatattg
     9541 ttgaggataa aacataatag ctaataaaaa taacattgat atgtttaact gaatgcataa
     9601 aatttttaat caagagttaa gatgagtcaa tgttaagtaa taaatacaca agtgttttta
     9661 atttaaaatg tggttttatt ataactttgg gatagactta taatgtcgcc agcaatatga
     9721 acatgcttga attgaagagt tgtctcaagt aaaatagacg ggtagatgaa aacaaactga
     9781 aggagtcagt aataatgaac tttaaagaaa tcaaagaatt aattgaaatt ctggataaat
     9841 caactttaac ggaaatcaat attgaagata ctaaaggcaa agtgacgctt aagaaagaaa
     9901 aagaaactga gattatcacg ccacaaatct cacaaatgcc agttgaagct gcggcaatgc
     9961 ctatgcctca agcacaatca actgatagca ataaaactga agctccaaag ccaacttcag
    10021 ataatcacaa aacaattaat gcacctatgg taggtacatt ttacaaatcg ccatctccag
    10081 acgaagaagc atatgtgcaa gttggggaca ctgtttcaaa tgaaacaaca gtgtgtattt
    10141 tagaggcaat gaaactattt aatgaaattc aagcagaaat ttcaggtgaa attgttgaaa
    10201 tcttagtaga agacggacaa atggtagagt atggccaacc gttatttaag gtgaaataat
    10261 gaaaaaggtt ttaattgcaa accgcggtga aatcgcagtt aggattattc gcgcttgtcg
    10321 tgatttaggc atccaaactg ttgcaatcta ttctgaaggg gataaagatg cgctacatac
    10381 tcaaattgct gatgaagcat attgcgtagg tcccactttg tctaaagatt catatttaaa
    10441 tattccgaac atcttatcta ttgcaacttc tacaggttgt gatggcgttc atccgggtta
    10501 tggcttttta gctgaaaatg ctgattttgc agaattatgc gaagcatgcc aattgaagtt
    10561 cattggacca agttatcaat ctatccaaaa aatgggtatc aaagatgttg ctaaggcaga
    10621 aatgatcaaa gccaatgttc cagttgttcc tggtagtgac ggtttaatga aagacgtctc
    10681 agaagctaag aaaatcgcca aaaaaattgg ctatccggtc atcattaaag ctactgctgg
    10741 cggtggcgga aaaggtatcc gtgttgctcg tgatgaaaaa gaacttgaaa ctggcttccg
    10801 aatgacagaa caagaagctc aaactgcatt tggtaatggt ggactttata tggagaaatt
    10861 catcgaaaac ttccgccata ttgaaatcca aattgttggg gacagctatg gtaatgtaat
    10921 tcatttagga gaacgtgatt gtacaattca aagacgtatg cagaaattag tggaagaagc
    10981 accttcccca attttagatg atgaaacacg tcgtgaaatg ggaaatgccg cagttcgtgc
    11041 agcgaaagct gtaaattatg aaaatgcggg aacaattgag tttatatatg atttaaatga
    11101 taataaattt tattttatgg aaatgaatac acgtattcaa gtagaacatc ctgtaactga
    11161 aatggtaaca ggaattgatt tagttaaatt acaattacaa gttgctatgg gtgacgtgtt
    11221 accgtataaa caagaagata ttaaattaac aggacacgca attgaattta gaattaatgc
    11281 tgaaaatcct tacaagaact ttatgccatc accaggtaaa attgagcaat atcttgcacc
    11341 aggtggatat ggtgttcgaa tagagtcagc atgttatact aattatacga taccgccata
    11401 ttatgattcg atggtagcga aattaatcat acatgaaccg acacgagatg aagcgattat
    11461 ggctggcatt cgtgcactaa gtgaatttgt ggttcttggt attgatacaa ctattccatt
    11521 ccatattaaa ttattgaata acgatatatt tagaagcggt aaatttaata caaacttttt
    11581 agagcaaaat agcattatga atgatgaagg ttaataggag gtcaatccat ggtcaaagta
    11641 actgattatt caaattcaaa attaggtaaa gtagaaatag cgccagaagt gctatctgtt
    11701 attgcaagta tagctacttc ggaagtcgaa ggcatcactg gccattttgc tgaattaaaa
    11761 gaaacaaatt tagaaaaagt tagtcgtaaa aatttaagcc gtgatttaaa aatcgagagt
    11821 aaagaagatg gcatatatat agatgtatat tgtgcattaa aacatggtgt taatatttca
    11881 aaaactgcaa acaaaattca aacgtcaatt tttaattcaa tttctaatat gacagcgata
    11941 gaacctaagc aaattaatat tcacattaca caaatcgtta ttgaaaagta atgtcatacc
    12001 taattcagta attaaataaa gaaaaataca aacgtttgaa ggagttaaaa atgagtcgta
    12061 aagaatcccg agtgcaagct tttcaaactt tatttcaatt agaaatgaag gacagtgatt
    12121 taacgataaa tgaagcgata agctttatta aagacgataa tccagattta gacttcgaat
    12181 ttattcattg gctagtttct ggcgttaaag gtcacgaacc tgtattagac gagacaatta
    12241 gtccttattt aaaagattgg actattgcac gtttattaaa aacggatcgt attattttaa
    12301 gaatggcaac atatgaaata ttacacagtg atacacctgc taaagtcgta atgaatgaag
    12361 cagttgaatt aacaaaacaa ttcagtgatg atgatcatta taaatttata aatggtgtat
    12421 tgagtaatat aaaaaaataa aattgagtga tgttatatgt cagattattt aagtgtttca
    12481 gctttaacga aatatattaa atataaattt gatcaagatc cacatctcca atctgtattg
    12541 attaaaggtg agctttcaaa tttcaaaaag cattcaagtg gtcatttata ctttaatgtg
    12601 aaagataaag aaagcgttat aagtgccatg atgttcaaag gtagtgcttc taaattaaac
    12661 ttcgaaccta aagaaggaga tgaagtctta ttagaagcac gtgtttctgt ttttgaacgt
    12721 cgtggaaact atcaaattta tgtaaataaa atgcaattag atggtatagg gaacttatat
    12781 caaaaattag aagcattaaa gaaaaaatta actgaagaag gttgttttga taaagcgaat
    12841 aagaaatcaa tacctaagtt tcctaaaaaa attgcagttt taacagcgag tacaggtgcc
    12901 gcaattcgag atatccattc aacgataaat agtcgttttc cattagctga acaaatacaa
    12961 attagtactt tggttcaagg tgaaaaagca aaagacgaca ttattgaaaa aattgaatat
    13021 gcagatagtt taggtgtaga taccattatt gtaggtcgag gcggtggatc catagaagat
    13081 ttatggaatt tcaacgaaga agctgtcgtc cgtgcaatat ataattgtaa gacaccgatt
    13141 atatcagcag ttggtcatga aacagacttt acattaagtg attttgctgc agacatcaga
    13201 gctgcgactc caactcaagc tgctgttatt gcaacaccag accaatatga attgctgcaa
    13261 caaatacagc aatatcagtt tacattgact cgtttcatca agaaacattt agaacaacaa
    13321 cgtaaacatg ttgaacattt gtcatcgtat tacaagttta aacaaccaac tttattgtat
    13381 gatcaacaga tacaacgtcg agatgattta gaaaagagac tgaaacaaca aattcaggca
    13441 acttttgaac aacagagaca tcgattaatg ctgttgcaac aacgttataa tttaaaagca
    13501 ttactaagta gtgttaatca agaacaacaa aataatcttc aattgacaaa ccaacttgtt
    13561 aaattattga attctaagat attaagttat aaaaatgatt tgaaaaataa agtagaaaac
    13621 ttaaacaatt taagtccaac taatacaatg ttgcgtggat atgcaattgt taataaaaaa
    13681 gacgaagtca ttacgagtac taaagattta acagaaaatg atcaattgac attaacaatg
    13741 aaagatggct tagtagatgc aaaagttacg aaagtaaggt gtaataatga ctaaagaaac
    13801 gcaaagtttt gaagaaatga tgcaagaatt agagcaaatt gttcaaaaat tagataatga
    13861 aacagtatct ttagaggaat cattagattt atatcaacgt ggtatgaaac tatcagcagc
    13921 ttgtgacaca actttaaaaa atgccgaaaa aaaggtgaat gacttaataa aagaagaagc
    13981 tgaggatgta aaaaatgacg aatctaccga tgaataaatt aatagatgaa gtcaataatg
    14041 aattatcggt tgcgataaat aaatcagtaa tggatactca gctagaagaa agtatgttgt
    14101 attcattaaa tgctggaggt aaacgcatcc gaccagttct gttattactc actttagatt
    14161 cactaaatac cgagtatgag ttaggtatga agagcgcaat tgcactagaa atgattcata
    14221 catattcact tattcatgat gacctaccag cgatggataa tgatgattat cgacgaggaa
    14281 aattaacaaa tcataaagta tatggtgagt ggactgcgat attagcaggt gatgctttat
    14341 taactaaagc atttgaactt atttcaagtg atgatagatt aactgatgaa gtaaaaataa
    14401 aagttctaca acggctgtca atagcaagtg gtcatgttgg aatggtcggc ggtcaaatgt
    14461 tagatatgca aagcgaaggc caaccaattg atcttgaaac tttggaaatg atacacaaaa
    14521 caaaaacagg agcattatta acttttgcgg ttatgagtgc agcagatatc gctaatgtcg
    14581 atgatacaac taaagaacat ttagaaagtt atagttatca tttaggtatg atgttccaga
    14641 ttaaagatga tttattagac tgctatggtg atgaagcaaa gttaggtaaa aaagtgggca
    14701 gcgatcttga aaataataaa agtacgtacg tgagtttatt agggaaagat ggcgcagaag
    14761 ataaattgac ttatcataga gacgcagcag tggatgaact aacgcaaatt gatgaacaat
    14821 tcaatacaaa acacttatta gaaatcgttg atttatttta tagcagggat cactaaaagc
    14881 aaatatatcg attagcaaca attgtaaagt ttgaaatgtc tcaaaaggca tattcaactg
    14941 acgttgtttg ttaatcgatt tttctcatgt tgaaacttaa aattatgttg aagactttca
    15001 tttaaaaaat agaattttca atttctatac gacaatattc gtatctgtga tatcaatgtg
    15061 attttaatta aatatgttca aacaattgac gcttattaaa tct
//
LOCUS       DALAGD010000074        14632 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.075, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000074 DALAGD010000000
VERSION     DALAGD010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14632
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.075"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..935
                     /gene="lukS-PV"
                     /locus_tag="O2701_001939"
     CDS             <1..935
                     /gene="lukS-PV"
                     /locus_tag="O2701_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Panton-Valentine bi-component leukocidin subunit
                     S"
                     /protein_id="HCZ4293822.1"
                     /translation="KKRLLAATLSLGIITPIATSFHESKADNNIENIGDGAEVVKRTE
                     DTSSDKWGVTQNIQFDFVKDKKYNKDALILKMQGFINSKTTYYNYKNTDHIKAMRWPF
                     QYNIGLKTNDPNVDLINYLPKNKIDSVNVSQTLGYNIGGNFNSGPSTGGNGSFNYSKT
                     ISYNQQNYISEVERQNSKSVQWGIKANSFITSLGKMSGHDPNLFVGYKPYSQNPRDYF
                     VPDNELPPLVHSGFNPSFIATVSHEKGSGDTSEFEITYGRNMDVTHATRRTTHYGNSY
                     LEGSRIHNAFVNRNYTVKYEVNWKTHEIKVKGHN"
     gene            931..1914
                     /gene="lukF-PV"
                     /locus_tag="O2701_001940"
     CDS             931..1914
                     /gene="lukF-PV"
                     /locus_tag="O2701_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panton-Valentine bi-component leukocidin subunit
                     F"
                     /protein_id="HCZ4293823.1"
                     /translation="MDMKKIVKSSVVTSIALLLLSNTVDAAQHITPVSEKKVDDKITL
                     YKTTATSDSDKLKISQILTFNFIKDKSYDKDTLILKAAGNIYSGYTKPNPKDTISSQF
                     YWGSKYNISINSDSNDSVNVVDYAPKNQNEEFQVQQTVGYSYGGDINISNGLSGGGNG
                     SKSFSETINYKQESYRTSLDKRTNFKKIGWDVEAHKIMNNGWGPYGRDSYHSTYGNEM
                     FLGSRQSNLNAGQNFLEYHKMPVLSRGNFNPEFIGVLSRKQNAAKKSKITVTYQREMD
                     RYTNFWNQLHWIGNNYKDENRATHTSIYEVDWENHTVKLIDTQSKEKNPMS"
     gene            2163..2348
                     /locus_tag="O2701_001941"
     CDS             2163..2348
                     /locus_tag="O2701_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293824.1"
                     /translation="MFILNTQAHARLDQMAQQFEVVCNGLNENEGQAIQTMDQSASLI
                     RSNLIQVKEQLEKLAVY"
     gene            2439..3356
                     /locus_tag="O2701_001942"
     CDS             2439..3356
                     /locus_tag="O2701_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HCZ4293825.1"
                     /translation="MFKRTKLILIATLLLSGCSTTNNESNKETKSVPEEMEASKYVGQ
                     GFQPPAEKDVVEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVVGSGKGVEVFVHCDDH
                     DIVFNASIPFDKSIIESDSSLRSEDKGDDMSTLVGTVLSGFEYRTQKEKYDNLYKFFK
                     DNEEKYQYTGFTKEAINKTQNVGYKNEYFYITYSSRSLKEYRKYYEPLIHKNDKEFKE
                     GMEQARKEVNYAANTDTVTTLFSTKENFTKDNTVDDVIELSDKLYNFKNKPEKSTITI
                     QIGKPTINTKKAFYDDNDPIEYGVYRKDE"
     gene            3413..4351
                     /locus_tag="O2701_001943"
     CDS             3413..4351
                     /locus_tag="O2701_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HCZ4293826.1"
                     /translation="MFKKAKLILIATLLLSGCSAMNNESKKDTNTETNTETKSVPEEM
                     EASKYVGQGFQPPAEKDAIEFVKKHRKEFEKVGEQFFKDNFGLKVKATNVVGKDDGVE
                     VYVHCEDHGIVFNASLPLYKDAIHQKGSMRSNDNGDDMSMMVGTVLSGFEYRAQKEKY
                     DNLYKFLKENEKQYQYTGFTKEAINKTQNVGYQNEYFYITYLSRNLKEYRKYYEPLIH
                     KNDKEFKEGMQRARKELNYTANTNTVATLFSTNDERNRKEKINNVIDLSEKIERTKDM
                     PIKNTITTQLGNKLIGTKKARFDDKKVVSFGAFEDE"
     gene            4344..4550
                     /locus_tag="O2701_001944"
     CDS             4344..4550
                     /locus_tag="O2701_001944"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293827.1"
                     /translation="MNKINDRDLTELSSYRVYQDINKDNDFTVNEKRFKQADVFEDLY
                     REKLKDTNKLREYNYLQNETFKSA"
     gene            4565..5479
                     /locus_tag="O2701_001945"
     CDS             4565..5479
                     /locus_tag="O2701_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HCZ4293828.1"
                     /translation="MLKKAKFILMATILLSGCSTTNNESNKETKSVPEEMDASKYVGQ
                     GFQPPAEKDAIEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVIGSGDGVEVFVHCDDH
                     DIVFNASIPFDKSIIDSDSSLRSKDKGDDMSTLVGAVLSGFEYRAQKEKYDKLYKFFK
                     DNEEKYQYTGFTKEAINKTQNSGYENEYFYISAIPYNLAEYRDYFEPLLNKSDSEFSK
                     ELSNVKKQLKDKSKVSVTTTLFSKKKNYTKKSNSENVIKMAEEIKKDKEIPNGIELSI
                     KFSDNKINTVKPNFNGESTSEYGVFDQE"
     gene            5678..5797
                     /locus_tag="O2701_001946"
     CDS             5678..5797
                     /locus_tag="O2701_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293829.1"
                     /translation="MTIFTQLSDRIKKAISKINQANNIPNNARKSPMYHSPIT"
     regulatory      5880..6014
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6076..6621
                     /locus_tag="O2701_001947"
     CDS             6076..6621
                     /locus_tag="O2701_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCZ4293830.1"
                     /translation="MQQNKRLITISMLSAIAFVLTFIKFPIPFLPPYLTLDFSDVPSL
                     LATFTFGPVAGIVVALVKNLLNYLFSMGDPVGPFANFLAGASFLLTAYAIYKNKRSTK
                     SLITGLIIATIVMTIVLSILNYFVLLPLYGMIFNLADIANNLKVIIVSGIIPFNIIKG
                     IVISIVFILLYRRLANFLKRI"
     gene            complement(6727..6975)
                     /locus_tag="O2701_001948"
     CDS             complement(6727..6975)
                     /locus_tag="O2701_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HCZ4293831.1"
                     /translation="MAKYTIVDMDTCIACGACGAAAPDIYDYDDEGIAFVILDDNQGT
                     AEVPEELYEDMEDAIDGCPTDSIKIADESFDGDALKFE"
     gene            7083..8036
                     /locus_tag="O2701_001949"
     CDS             7083..8036
                     /locus_tag="O2701_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCZ4293832.1"
                     /translation="MQHIIKTALQQTFNYKTNKSIYNILVGKKSHQTFFDACSQQQLS
                     LYHSLPLLKYPSFELFLEKINEFNAEMEIMLHPRYTFESMGQTFQAIQLLVQTMSNTK
                     QHVFHFVPISQNNKIQETVKIVYNYIKENQLQIDFENELHNLFKAITLKGPCYLHYYL
                     QGYDEPMYTRQQVSLIEKLSQQQLFEYEMNNLVTMMFELESGEYTILSKIIMKPTLLN
                     QTYITYTKLLEQFTMEDIAAQQQVKINTIEDHVLEILIKGYMSNYDDYVELEDQLQFL
                     NFYQQHRGERLKFYKEQFDTLSYFQLKVLIVGFERGDLNVA"
     gene            8026..9405
                     /locus_tag="O2701_001950"
     CDS             8026..9405
                     /locus_tag="O2701_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCZ4293833.1"
                     /translation="MLHDILRNKFGFESFKPGQQEIIESIMSQQHTLGILPTGSGKSL
                     CYQIPTYLSGKPTLIISPLISLMDDQVMQLKINGEKRVTCIHSGMDEIEKKHNIKCLR
                     HSRFIFLSPEFLLQPSNFKLISMIDFGMIVLDEAHCLSEWGYDFRPHYALIGKVTKHF
                     KEAVVLALTATAPPHLQDDLTEMLAIQFNVIKTTMNRPNISFKHLNFHDDEDKIEWLL
                     PFLQQSGPTIIYVSSKKMCLNLAQLIYDSGFLTGIYHGDMNYQERHTVQQQFLNNDIP
                     IIVATSAFGMGINKKDIRTIIHFHLSTSPSNYIQEIGRAGRDGELSQAISLFQPDDKY
                     ILETLLFADMITEEDVQNFEIGEFLAPDKQAVLTTLQSFYSIGALKQIFKQSFKRKQL
                     GFFRMIGYCKLDQCRRKYLLEFFGEYPPAQDRCCDNDSNITDIAILNKKKVIRSIGFD
                     EKLQNLFLR"
     gene            9558..11018
                     /gene="ebpS"
                     /locus_tag="O2701_001951"
     CDS             9558..11018
                     /gene="ebpS"
                     /locus_tag="O2701_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elastin-binding protein EbpS"
                     /protein_id="HCZ4293834.1"
                     /translation="MSNNFKDDFEKNRQSIDTNSHQDHTEDVEKDQSELEHQDTIENT
                     EQQFPPRNAQRRKRRRDLATNHNKQVHNESQTSEDNVQNEAGTIDDRQVESSHSTESQ
                     EPSHQDSTPQHEEEYYNKNAFAMDKSHPEPIEDNDKHDTIKNAENNTEHSTVSDKSEA
                     EQSQQPKPYFTTGANQSETSKNEHDNDSVKQDQDEPKEHHNGKKAAAIGAGTAGVAGA
                     AGAMAASKAKKHSNDAQNKSNSGKANNSTEDKASQDKSKDHHNGKKGAAIGAGTAGLA
                     GGAASKSASAASKPHASNNASQNHDEHDNHDRDKERKKGGMAKVLLPLIAAVLIIGAL
                     AIFGGMALNNHNNGTKENKIANTNKNNADESKDKDTSKDASKDKSKSTDSDKSKEDQD
                     KATKDESDNDQNNANQANNQAQNNQNQQQANQNQQQQQQRQGGGQRHTVNGQENLYRI
                     AIQYYGSGSPENVEKIRRANGLSGNNIRNGQQIVIP"
     gene            11143..11259
                     /locus_tag="O2701_001952"
     CDS             11143..11259
                     /locus_tag="O2701_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293835.1"
                     /translation="MLFNKVFSRILAYQFNFLNSHQKDKTPQSTNLTRKNKK"
     gene            11420..12406
                     /locus_tag="O2701_001953"
     CDS             11420..12406
                     /locus_tag="O2701_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HCZ4293836.1"
                     /translation="MQKVESIIIGGGPCGLSAAIEQKRKGIDTLIIEKGNVVESIYNY
                     PTHQTFFSSSDKLSIGDVPFIVEESKPRRNQALVYYREVVKHHQLKVNAFEEVLTVKK
                     MNNKFTITTTKDVYECRFLTIATGYYGQHNTLEVEGADLPKVFHYFKEAHPYFDQDVV
                     IIGGKNSAIDAALELEKAGANVTVLYRGGDYSPSIKPWILPNFTALVNHEKIDMEFNA
                     NVTQITEDTVTYEVNGESKTIHNDYVFAMIGYHPDYEFLKSVGIQINTNEFGTAPMYN
                     KETYETNIENCYIAGVIAAGNDANTIFIENGKFHGGIIAQSMLAKKQTPLES"
     gene            complement(12521..13489)
                     /locus_tag="O2701_001954"
     CDS             complement(12521..13489)
                     /locus_tag="O2701_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCZ4293837.1"
                     /translation="MKHLLVIHTGGTISMSQDQSNKVVTNDINPISMHQDVINQYAQI
                     DELNPFNVPSPHMTIQHVKQLKDIILEAVTNKYYDGFVITHGTDTLEETAFLLDLILG
                     IEQPVVITGAMRSSNEIGSDGLYNYISAIRVASDEKARHKGVMVVFNDEIHTARNVTK
                     THTSNTNTFQSPNHGPLGVLTKDRVQFHHMPYRQQALENVNDKLNVPLVKAYMGMPGD
                     IFSFYSREGIDGMVIEALGQGNIPPSALEGIQQLVSLNIPIVLVSRSFNGIVSPTYAY
                     DGGGYQLAQQGFIFSNGLNGPKARLKLLVALSNNLDKAEIKSYFEL"
     gene            13566..14225
                     /locus_tag="O2701_001955"
     CDS             13566..14225
                     /locus_tag="O2701_001955"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCZ4293838.1"
                     /translation="MKAINIALDGPAAAGKSTIAKRVASELSMIYVDTGAMYRALTYK
                     YLKLNKTEDFAKLVDQTTLDLTYKADKGQCVILDNEDVTDFLRNNDVTQHVSYVASKE
                     PVRSFAVKKQKELAAEKGIVMDGRDIGTVVLPDADLKVYMIASVEERAERRYKDNQLR
                     GIESNFEDLKRDIEARDQYDMNREISPLRKADDAVTLDTTGKSIEEVTDEILAMVSQI
                     K"
     gene            14256..14372
                     /locus_tag="O2701_001956"
     CDS             14256..14372
                     /locus_tag="O2701_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293839.1"
                     /translation="MQRLTHIQNECADNDLLKLKGDFYSMMNESCLFIFGKS"
     gene            14489..>14632
                     /locus_tag="O2701_001957"
     CDS             14489..>14632
                     /locus_tag="O2701_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293840.1"
                     /translation="MSVTNLNIAKEADLVTKNVLCNNSRKMHYIRSSYYSNVKVAYLR
                     GFLF"
BASE COUNT         5672 a         2122 c         2505 g         4333 t
ORIGIN      
        1 tcaaaaaaag actattagct gcaacattgt cgttaggaat aatcactcct attgctactt
       61 cgtttcatga atctaaagct gataacaata ttgagaatat tggtgatggc gctgaggtag
      121 tcaaaagaac agaagataca agtagcgata agtggggggt cacacaaaat attcagtttg
      181 attttgttaa agataaaaag tataacaaag acgctttgat tttaaaaatg caaggtttta
      241 tcaattcaaa gactacttat tacaattaca aaaacacaga tcatataaaa gcaatgaggt
      301 ggcctttcca atacaatatt ggtctcaaaa caaatgaccc caatgtagat ttaataaatt
      361 atctacctaa aaataaaata gattcagtaa atgttagtca aacattaggt tataacatag
      421 gtggtaattt taatagtggt ccatcaacag gaggtaatgg ttcatttaat tattcaaaaa
      481 caattagtta taatcaacaa aactatatca gtgaagtaga acgtcaaaat tcaaaaagtg
      541 ttcaatgggg aataaaagct aattcattta tcacatcatt aggtaaaatg tctggacatg
      601 atccaaattt atttgttgga tataaaccat atagtcaaaa tccgagagac tattttgttc
      661 cagacaatga attaccccca ttagtacaca gtggtttcaa tccttcattt attgcaactg
      721 tttctcatga aaaaggctca ggagatacaa gtgaatttga aataacgtat ggcagaaata
      781 tggatgttac tcatgctact agaagaacaa cacactatgg caatagttat ttagaaggat
      841 ctagaataca caacgcattt gtaaacagaa attacacagt taaatatgaa gtgaactgga
      901 aaactcatga aattaaagtg aaaggacata attgatatga aaaaaatagt caaatcatca
      961 gttgttacat caattgcatt gcttttgcta tccaatacag ttgatgcagc tcaacatatc
     1021 acacctgtaa gtgagaaaaa ggttgatgat aaaattactt tgtacaaaac aactgcaaca
     1081 tcagattccg ataagttaaa aatttctcag attttaactt ttaattttat taaagataaa
     1141 agttatgata aagatacatt aatactcaaa gctgctggaa acatttattc tggctataca
     1201 aagccaaatc caaaagacac tattagttct caattttatt ggggttctaa gtacaacatt
     1261 tcaattaatt cagattctaa tgactcagta aacgttgtag attatgcacc taaaaatcaa
     1321 aatgaagaat ttcaagtaca acaaacggta ggttattctt atggtggaga tattaatatc
     1381 tctaacggct tatcaggtgg aggtaatggt tcaaaatctt tttcagagac aattaactat
     1441 aaacaagaaa gctatagaac tagcttagat aaaagaacta atttcaaaaa aattggttgg
     1501 gatgttgaag cacataaaat tatgaataat ggttggggac catatggcag agatagttat
     1561 cattcaactt atggtaatga aatgttttta ggctcaagac aaagcaactt aaatgctgga
     1621 caaaacttct tggaatatca caaaatgcca gtgttatcca gaggtaactt caatccagaa
     1681 tttattggtg tcctatctcg aaaacaaaac gctgcaaaaa aatcgaaaat tactgttact
     1741 tatcaaagag aaatggatag atatacaaac ttttggaatc aacttcactg gataggtaat
     1801 aattataaag atgaaaatag agcaactcat acatcaattt atgaagttga ttgggaaaat
     1861 catacagtta aattaataga tactcaatct aaggaaaaaa atcctatgag ctaaacagat
     1921 agataatcaa aaaatcttaa atatgttaaa atttacaaac actttctttc tatattaggg
     1981 taaccacgtc ttaattgacg tggttatttt ttcagggcaa aaaaagggcg gattatttaa
     2041 ataaggacaa acacttgtgg aaaatttaaa aggttaaaaa taataaagaa cttggtataa
     2101 caagggtttt atacatttgc gtacaacgac gaaatgtcaa tttaccatca cattatgacg
     2161 atatgtttat tttaaacaca caagctcatg cgcgtcttga tcaaatggca caacagtttg
     2221 aagttgtttg taatggcttg aacgaaaatg aaggacaagc aattcaaacg atggatcaat
     2281 ctgcctctct aatacggtca aacttaattc aagttaaaga acaattagaa aaactagctg
     2341 tatactaatt aatttattaa atgctacttg ttttcattga gaataagtag ctttttcaaa
     2401 cataaaagtt ttacaaacac ataaatgggt gatgagctat gtttaaaaga actaaactaa
     2461 tcttaatagc aacattactg ctatcaggat gttcaactac caataacgaa tctaataaag
     2521 aaacaaaatc agtgccagaa gaaatggaag cttcaaaata tgtaggtcaa ggcttccaac
     2581 cacctgcaga aaaagatgtg gttgaatttg cgaaaaagca taaagataaa attgccaaac
     2641 gaggcgaaca attttttatg gataatttcg gtctaaaagt taaagctacc aatgttgtag
     2701 gtagtggaaa aggcgtagaa gtattcgtgc attgtgatga ccacgatatc gtatttaatg
     2761 cgagtattcc atttgataaa tcaataattg agagtgatag ctcattaaga agtgaggaca
     2821 aaggcgatga tatgagtact ttagttggta cagtgttgag tggctttgaa tatcgaacac
     2881 aaaaagaaaa gtatgacaat ttatataaat ttttcaaaga taatgaagag aaataccaat
     2941 atacaggctt tacaaaagaa gcaataaaca agacgcaaaa tgtcggatat aaaaatgaat
     3001 atttttatat cacttactcg tcaagaagct taaaagaata tcgtaagtat tacgaaccat
     3061 tgattcataa aaatgataaa gaatttaaag aaggaatgga acaagccaga aaagaagtga
     3121 attacgctgc taatacagat acagtaacaa cattgtttag tacaaaggaa aattttacta
     3181 aagacaatac agttgatgat gtaattgaac tgagtgataa actatataat tttaaaaata
     3241 agccagaaaa atctacaatt acaattcaaa taggaaagcc tactattaat actaaaaaag
     3301 ccttttatga tgataatgat ccaatagaat atggagtgta tcgtaaagat gaataaattt
     3361 ttttaaaatg ctttttaata ttctatataa acacataaat gggtgatgag ctatgtttaa
     3421 aaaggcgaaa ttaatcttaa tagcaacgct actgttatca ggatgttcag cgatgaataa
     3481 tgaatcaaaa aaagacacaa atacagaaac aaatacagaa acaaaatcag taccagaaga
     3541 aatggaagct tcaaaatatg taggacaagg ctttcaacca cctgcagaaa aagatgcgat
     3601 tgaatttgtg aagaagcatc gtaaagaatt tgaaaaagta ggtgaacaat tctttaaaga
     3661 taactttgga ctaaaagtta aagctacaaa tgttgtaggt aaagatgatg gtgtagaagt
     3721 ttatgtgcat tgcgaagatc atggcattgt atttaatgca agtctacctt tgtacaaaga
     3781 tgccatccat caaaaaggat caatgcgtag taatgacaac ggtgatgata tgagtatgat
     3841 ggtgggtaca gtgctgagtg gctttgaata tcgagcgcaa aaagaaaagt atgataattt
     3901 atataaattt ttaaaagaaa atgaaaagca atatcaatat acaggtttta ctaaagaagc
     3961 aattaacaag acgcaaaacg tcgggtatca aaatgaatat ttttatatta catatttatc
     4021 aagaaattta aaagaatata gaaaatatta cgaaccattg atacataaaa atgataaaga
     4081 gtttaaagaa ggtatgcaac gagctagaaa agagctaaac tatactgcta atacaaatac
     4141 tgtagcaacg ttgtttagta cgaatgatga aaggaataga aaagaaaaga taaataatgt
     4201 aatagattta tccgagaaaa ttgaaagaac aaaagatatg ccaatcaaga atactataac
     4261 tactcaatta ggaaataaac ttattggcac aaaaaaagct cgttttgatg ataagaaagt
     4321 agtgtcgttt ggagcatttg aagatgaata aaataaatga tagagattta acagaattga
     4381 gtagctatag ggtttatcaa gacatcaata aagataatga ctttacagtt aacgaaaaac
     4441 gatttaagca ggcagatgta tttgaagatt tatatagaga gaaactaaaa gacacaaata
     4501 aattaagaga gtataattat ttacaaaatg aaacttttaa aagcgcataa ataggtgatg
     4561 agatatgctt aaaaaagcaa aatttatctt aatggcaacg atactactat caggatgttc
     4621 aactaccaat aacgaatcca acaaagaaac aaaatctgta ccagaagaaa tggatgcttc
     4681 aaaatatgta ggacaaggat tccaaccacc tgcagaaaaa gatgcgattg aatttgcaaa
     4741 gaagcataaa gataaaattg ctaagcgagg cgaacaattt tttatggata acttcggtct
     4801 aaaagttaaa gctacaaatg ttataggtag tggcgatggt gtagaagtat tcgtgcattg
     4861 tgatgaccac gacatcgtat ttaatgcgag tattccattt gataaatcaa ttattgacag
     4921 tgatagctca ttaagaagta aggataaagg tgatgatatg agtactttag ttggtgcagt
     4981 actcagtggg tttgaatatc gagcacaaaa agaaaaatat gataaattat ataaattttt
     5041 caaagataat gaagagaaat atcaatatac aggatttaca aaagaagcaa ttaataagac
     5101 gcaaaatagt ggttatgaaa atgaatattt ttatatttcg gccatacctt ataatttagc
     5161 tgagtataga gactattttg aaccattgtt aaacaaaagt gacagtgaat tttcaaaaga
     5221 attgtcaaat gttaagaagc aattaaaaga taagtctaaa gtttcggtaa ctactactct
     5281 atttagtaaa aaaaagaact atactaaaaa aagtaacagt gaaaatgtaa taaaaatggc
     5341 agaagaaata aaaaaagata aagagatacc aaacggtata gagcttagta taaaattttc
     5401 ggacaataaa ataaatacgg ttaaaccaaa ttttaacggt gaaagcactt cagaatatgg
     5461 tgtgtttgat caagaataaa attaatgatg aaaatttaac ggagaatagt gtatattgag
     5521 tagatcaaga ataaaaagat aattctacta ttgttgtgaa ggcaaataag tagaagattt
     5581 taagtgtaat ttctggtgat ttaaataata atataaatgg aagtactgat ataaaacttt
     5641 ttaacctact agattcttat aatttgcttt ccattttatg acgattttta ctcaattgag
     5701 tgatagaatc aaaaaagcca tctcaaaaat taatcaagca aacaacattc caaacaatgc
     5761 tcgcaaatca ccaatgtatc actctccaat tacgtaacta tgatttaatt taagcatagt
     5821 tattgaggtt ttgtgatata tagtataaaa ttaatgagaa ttaaatttaa taatgtaaaa
     5881 ttcatcttcg gggtcgggtg taattcccaa ccggcagtaa ataaagcctg cgacctgcta
     5941 gtatgtatca tattagtggc tgatctagtg agattctaga gccgacagta tagtctggat
     6001 gggagaagat ggaggttttt tgttgtgcaa taatcctcct attcttacga gatgaatgga
     6061 aggagaaaat tgaatatgca acaaaataaa cgtcttatca caataagtat gttgagcgcg
     6121 attgcgtttg tgttaacttt tatcaagttt cctataccat ttttgccacc atacttaact
     6181 ttagatttta gtgatgtacc gtcactacta gctacattta cgtttggacc agttgccggt
     6241 atcgtagttg cactggttaa aaatttattg aactacttat ttagtatggg cgatccagtt
     6301 ggaccatttg ctaacttttt agcaggcgca agtttcttat taactgctta cgccatctat
     6361 aaaaataaac gttcaacaaa atctttgatt actggattaa tcattgcaac aatcgttatg
     6421 actatcgtgt tgagtatttt gaactatttc gttctattac ctttgtacgg tatgatattt
     6481 aacttagctg atatcgcaaa taatcttaaa gtaatcattg tttcaggaat tataccattc
     6541 aatattatta aaggtatcgt tatttctatt gtatttattt tactatatag aaggcttgcg
     6601 aatttcttga aaagaattta atcaaattaa agcaaaataa tatacacata ataataaaaa
     6661 gcaggtgact atcaataaac gatagcttgc ctgctttttc tatagaacat ttgtctaaaa
     6721 aatcaattat tcaaatttta aagcgtcccc atcaaatgat tcgtctgcaa ttttaataga
     6781 atctgtaggg catccatcaa ttgcatcttc catatcttca tataattcct caggtacttc
     6841 tgcagtacct tggttatcgt caaggattac gaaagcaata ccttcgtcgt cgtaatcata
     6901 tatatctggc gctgctgcac cgcatgcacc acatgcaata caagtatcca tatcaacgat
     6961 tgtatatttt gccaatgtct tcgcctcctt tgataaaaat gctaaaatag taatgtgact
     7021 aaaattttag acagcatcat ttttattttc aaattatccg ttttacagag tgagggttaa
     7081 atttgcaaca cattataaaa acagcattac aacaaacatt taactataaa acaaataaaa
     7141 gtatttacaa tatcttagtt ggtaagaaat ctcaccaaac cttttttgac gcttgtagtc
     7201 aacaacagtt gtcattatat cacagtttac cactattaaa atatccgtct tttgagctat
     7261 ttttagaaaa aatcaatgaa tttaatgctg aaatggaaat catgttgcat cctagatata
     7321 catttgaaag catggggcaa acatttcaag caattcaact attagtgcaa accatgtcta
     7381 ataccaaaca acatgttttt cattttgtac caatctctca aaataataag atacaagaaa
     7441 ctgtaaagat tgtctataac tacattaaag aaaaccaatt acaaattgat tttgaaaatg
     7501 aactacataa cttgtttaaa gcaataactt taaaagggcc atgttactta cattattatt
     7561 tgcaaggcta tgatgaacca atgtatacga gacagcaagt tagtttaata gaaaagctat
     7621 ctcaacagca attgtttgaa tacgaaatga ataatttagt gacaatgatg tttgaattag
     7681 aaagtggaga atatactatt ttatcaaaaa taataatgaa acctacatta ttaaatcaaa
     7741 cttatattac ttatacaaaa ttgcttgaac aattcacgat ggaagatata gcggctcaac
     7801 aacaagttaa aatcaatact atcgaagatc atgtacttga aatcttaatc aaaggttaca
     7861 tgtctaatta cgatgattat gttgaactag aagatcaact ccagtttttg aatttttatc
     7921 aacagcatcg tggcgaacga ttaaaatttt acaaagaaca atttgacacg ttatcatatt
     7981 ttcaattaaa agtattaatc gttggatttg aaagaggtga tctgaatgtt gcatgatatt
     8041 ttacgaaaca aatttggatt cgagagtttt aaaccgggac aacaggaaat tatagaaagt
     8101 ataatgtctc aacaacacac tctaggtata cttccaactg gaagtggaaa gagtttgtgt
     8161 tatcaaatac ctacgtattt atcaggtaag ccgacattaa ttatctcacc gttaatatct
     8221 ttaatggatg accaagttat gcagttgaaa ataaatggag aaaaacgtgt aacatgtatt
     8281 cactctggta tggatgaaat tgagaaaaag cataatatta aatgtttacg acatagccgc
     8341 ttcatctttc taagtccaga atttctcctg caaccgtcaa attttaaatt aatatctatg
     8401 atagactttg gcatgattgt tctagatgaa gcacattgcc tatctgaatg gggatatgat
     8461 ttcagaccac attatgctct aataggaaaa gtaacaaagc attttaaaga agcggttgtc
     8521 ttagcattga cagcaactgc accaccgcat ttacaagatg atttgacgga aatgttagcg
     8581 attcaattca atgttattaa aactacaatg aatcgcccaa atataagctt taagcatctt
     8641 aattttcatg atgatgaaga taaaattgaa tggttgctgc cgtttctaca acagtcggga
     8701 ccaacgatta tttatgtctc atcgaaaaag atgtgtctga atttagcgca acttatttat
     8761 gattcaggtt ttcttacagg tatttatcat ggtgatatga attatcaaga gcgacacaca
     8821 gttcaacaac aatttttaaa taatgatatt ccgattatag tcgcaacgag tgcttttggt
     8881 atgggaatta ataaaaaaga tattcgcaca atcattcact ttcatctttc aacaagtcct
     8941 tctaactaca ttcaagaaat tggccgtgcg ggtcgcgatg gtgaactaag tcaggcaatt
     9001 agtttattcc aaccggacga taaatatatt ttagaaacgt tattatttgc agatatgata
     9061 acagaagaag atgtacaaaa tttcgaaata ggagaatttt tagctcccga taaacaagcc
     9121 gttttgacaa cgttgcaatc attctatagt atcggcgcct tgaaacagat atttaagcaa
     9181 tcatttaaac gaaagcaatt aggattcttt cgcatgattg gctattgcaa attggatcaa
     9241 tgtagacgga agtatttatt agaatttttc ggtgaatatc caccggcaca agatcgatgt
     9301 tgtgacaatg attctaatat aactgatatc gcaattttaa ataagaagaa ggtaattaga
     9361 agtattggat ttgatgaaaa gttgcaaaat ttatttctca gatagtatta ctttactaaa
     9421 agaaaattga caagctataa ttagtgtata cacaattgaa aaatgattga aataattttg
     9481 aaaaatatac ataaacatat gtcatgtggg tatattttat gtaaaatcat tgtaatagaa
     9541 tagaaaggaa gatggctatg tctaataatt ttaaagatga ctttgaaaaa aatcgtcaat
     9601 cgatagacac aaattcacat caagaccata cggaagatgt tgaaaaagac caatcagaat
     9661 tagaacatca ggatacaata gagaatacgg agcaacagtt tccgccaaga aatgcccaaa
     9721 gaagaaaaag acgccgtgat ttagcaacga atcataataa acaagttcac aatgaatcac
     9781 aaacatctga agacaatgtt caaaatgagg ctggcacaat agatgatcgt caagtcgaat
     9841 catcacacag tactgaaagt caagaaccta gccatcaaga cagtacacct caacatgaag
     9901 aggaatatta taataagaat gcttttgcaa tggataaatc acatccagaa ccaatcgaag
     9961 acaatgataa acacgatact attaaaaatg cagaaaataa cactgagcat tcaacagttt
    10021 ctgataagag tgaagctgaa caatctcagc aacctaaacc atattttaca acaggtgcta
    10081 accaatcaga aacatcaaaa aatgaacatg ataatgattc tgtaaaacaa gatcaagatg
    10141 aacctaaaga acatcataat ggtaaaaaag cagcagctat tggtgctgga acagcaggtg
    10201 ttgcaggtgc agctggtgca atggctgctt ctaaagctaa gaaacattca aatgacgctc
    10261 aaaacaaaag taattctggc aaggcgaata actcgactga ggataaagcg tctcaagata
    10321 agtctaaaga tcatcataat ggcaaaaaag gtgcagcgat cggtgctgga acagcaggtt
    10381 tggctggagg cgcagcaagt aaaagtgctt ctgccgcttc aaaaccacat gcctctaata
    10441 atgcaagcca aaaccatgat gaacatgaca atcatgacag agataaagaa cgtaaaaaag
    10501 gtggcatggc caaagtattg ttaccattaa ttgcagctgt actaattatc ggtgcattag
    10561 cgatatttgg aggcatggca ttaaacaatc ataataatgg tacaaaagaa aataaaatcg
    10621 cgaatacaaa taaaaataat gctgatgaaa gtaaagacaa agacacatct aaagacgctt
    10681 ctaaagataa atcaaaatct acagacagtg ataaatcaaa agaggatcaa gacaaagcga
    10741 ctaaagatga atctgataat gatcaaaaca acgctaatca agcgaacaat caagcacaaa
    10801 ataatcaaaa tcaacaacaa gctaatcaaa atcaacaaca gcaacaacaa cgtcaaggtg
    10861 gtggccaaag acatacagtg aatggtcaag aaaacttata ccgtatcgca attcaatact
    10921 acggttcagg ttcaccggaa aatgttgaaa aaattagacg tgccaatggt ttaagtggta
    10981 acaatattag aaacggtcaa caaatcgtta ttccataata taactatata aattgtaact
    11041 gaactgctat atacaaacgt gcatattata agtatctcta aatattttgg agaacttaaa
    11101 ttgcgcttat aagtatgtag cggttttttc atttttcaaa gtttgttatt taacaaggtc
    11161 ttttctcgaa tattggcata tcaatttaac tttttaaata gtcatcaaaa agataaaaca
    11221 ccacaatcaa caaatttaac gaggaagaat aaaaaataaa tcaacatatt aaattgtagt
    11281 gttattcaac tccgtagcta acaattctct atgcacatta aacaaattgt caaaaatata
    11341 tcataaatct tcaagcacag acttagcgca tcaatcactg aactgttata atagtttggg
    11401 attaaaggag gccgaaacaa tgcaaaaagt tgaaagtatc ataattggtg gagggccatg
    11461 cggattaagt gcggctattg aacaaaaaag aaaaggtatt gataccttaa ttattgaaaa
    11521 gggtaatgtc gttgaatcaa tctacaatta tcctactcac caaacatttt tctcatcaag
    11581 tgataaatta agtattgggg acgtaccgtt tatcgttgaa gaaagtaaac caagacgtaa
    11641 tcaagcgcta gtttattacc gagaagttgt aaaacatcat caattaaaag taaatgcatt
    11701 tgaagaagta ttaactgtta aaaaaatgaa taataaattt actattacta cgacgaaaga
    11761 tgtttatgaa tgtcgatttt taacaatcgc gacaggctat tatggtcagc ataatacatt
    11821 agaagttgaa ggtgcggatt tacctaaagt gttccattat tttaaagagg cacatccgta
    11881 ttttgatcaa gatgttgtaa ttatcggtgg taagaattcg gctatcgatg ctgctttgga
    11941 gttggaaaaa gctggtgcta acgtgacggt tctatatcgt ggtggagatt attcgccttc
    12001 aattaaaccg tggatacttc caaatttcac agcattagta aatcatgaaa aaattgacat
    12061 ggaatttaat gctaatgtta cccaaataac tgaagatact gtgacttatg aagtaaatgg
    12121 tgaaagtaaa acgatacaca atgattatgt atttgcgatg attggttatc atcccgatta
    12181 tgaattttta aaatctgtag gcattcaaat taatacaaat gaatttggaa cagcgcctat
    12241 gtataataaa gaaacatacg aaacaaatat cgaaaattgc tatattgcag gtgtaattgc
    12301 tgcagggaac gatgcgaata ccatttttat tgaaaatggt aaattccacg ggggcattat
    12361 tgctcaaagc atgctagcta agaaacaaac gcccttagaa tcataaaaat aaaggtctat
    12421 gtactaaata acttagtttt acaacgactg acattcatga tatgtcaaat gaggttgatg
    12481 actaatgatt gtacatagac ctttttatgt tacgtattca ttataattca aaatatgatt
    12541 tgatttcagc tttatctaaa ttgttgctta acgcgactaa taattttaat cttgcttttg
    12601 gaccattcaa gccgttagaa aaaataaaac cttgttgtgc gagttggtaa ccaccaccat
    12661 cgtatgcgta agttggactc acaataccat taaaggaacg tgaaactagc acaataggta
    12721 tatttaaaga tactaattgt tgaatgcctt ctaatgcgct tggaggtatg ttgccttgtc
    12781 ctaacgcttc aataaccata ccatcgatac cttctcgact ataaaaacta aaaatgtcac
    12841 ctggcatacc catatatgct tttactaatg gtacatttag tttgtcattg acattttcca
    12901 atgcttgttg gcgatatggc atatgatgga attgcacacg atcctttgtc aatacaccta
    12961 gcggaccatg atttggactt tgaaatgtgt ttgtattaga cgtatgtgtt ttggtaacat
    13021 tacgcgccgt atgaatttca tcattaaata caaccatcac gcctttatga cgggcctttt
    13081 catcagaggc aacgcgaata gcggaaatat aattatataa tccgtcagaa ccaatttcat
    13141 tagacgagcg cattgcgcca gtaataacaa caggttgctc gatacctaat attaaatcaa
    13201 gtaaaaaggc agtttcttct aacgtatcgg taccatgcgt gataacgaaa ccatcataat
    13261 atttatttgt tactgcttct aaaataatat cctttaattg tttaacatgt tggattgtca
    13321 tatgaggtga tggtacatta aaaggattta attcatctat ttgtgcatat tgatttatga
    13381 catcttgatg cattgaaata gggttgatat catttgttac tactttatta gattggtctt
    13441 gtgacatact aatggtgcca ccagtatgaa taacaagtag atgtttcata tatttcctcc
    13501 tatatttaat ttacctaatt atgataaaat attattcata aaacgacaag gaagggaaat
    13561 gacgcatgaa agccattaat attgcattag atggtccagc tgctgccgga aaaagtacaa
    13621 ttgcgaaacg tgtagccagc gaactatcaa tgatttatgt cgatacagga gcaatgtatc
    13681 gtgcattaac atacaaatat ttaaaattaa acaaaactga ggactttgca aaactagttg
    13741 accaaacaac attagattta acttataaag cagataaagg tcaatgtgtc attttagata
    13801 acgaagatgt aacagacttt ttaagaaata atgatgtgac gcaacatgtt tcatacgttg
    13861 catctaaaga gccagtacgt tcattcgccg ttaaaaaaca aaaagagtta gctgcagaaa
    13921 aaggtatcgt aatggatggt cgcgatatcg gaactgtagt gctaccagat gcagatttaa
    13981 aagtatatat gattgcatca gttgaagagc gagcagaaag aagatataaa gataatcaat
    14041 taagaggtat cgaatcaaat tttgaagatt taaaacgtga tattgaagct cgtgatcaat
    14101 atgacatgaa ccgtgaaata tcaccattaa gaaaagcaga tgatgcagtg acattagata
    14161 cgacaggcaa gtcgattgaa gaagttactg acgaaatttt agcgatggtg agtcaaatta
    14221 aataatctaa taagtcataa ctatctattt gtaatgtgca acgcttaacg catatacaaa
    14281 atgaatgtgc tgataatgat ttactcaaat taaaaggtga tttttattca atgatgaatg
    14341 aaagttgcct ttttattttt ggtaaaagtt aatgcgtcag tgaattgtgt aagtttttca
    14401 aaaagtaaaa agaaataata aaggtgaatt attagaattc caaaaataat tcattacatt
    14461 cataaagcat tttacaaatg gtaagaaaat gagtgttaca aatctaaata ttgcaaaaga
    14521 agctgattta gtcacaaaaa atgtcctatg taataattcg agaaagatgc actatatacg
    14581 gtcttcttac tattcaaatg taaaagttgc ttatttgcgt ggctttttgt tt
//
LOCUS       DALAGD010000075        14466 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.076, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000075 DALAGD010000000
VERSION     DALAGD010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14466
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.076"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(<1..566)
                     /locus_tag="O2701_001958"
     CDS             complement(<1..566)
                     /locus_tag="O2701_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HCZ4293841.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     RFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPEDLFSAMIR
                     EIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALIS
                     KVMVNLDDLPFVHSDMEIDMLGDAYEFL"
     gene            644..745
                     /locus_tag="O2701_001959"
     CDS             644..745
                     /locus_tag="O2701_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293842.1"
                     /translation="MENFDVGGGNFTFMEIGIGFMTHKPKRLNDDID"
     gene            complement(830..1513)
                     /locus_tag="O2701_001960"
     CDS             complement(830..1513)
                     /locus_tag="O2701_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL10"
                     /protein_id="HCZ4293843.1"
                     /translation="MKFTALAKATLALGILTTGTLTTEVHSGHAKQNQKSVNKHDKEA
                     LYRYYTGKTMEMKNISALKHGKNNLRFKFRGIKIQVLLPGNDKSKFQQRSYEGLDVFF
                     VQEKRDKHDIFYTVGGVIQNNKTSGVVSAPILNISKEKGEDAFVKGYPYYIKKEKITL
                     KELDYKLRKHLIEKYGLYKTISKDGRVKISLKDGSFYNLDLRSKLKFKYMGEVIESKQ
                     IKDIEVNLK"
     gene            complement(1879..2577)
                     /locus_tag="O2701_001961"
     CDS             complement(1879..2577)
                     /locus_tag="O2701_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL9"
                     /protein_id="HCZ4293844.1"
                     /translation="MKLTTIAKATLALGILTTGVFTAESQTGHAKVELDETQRKYYIN
                     MLHQYYSEESFEPTNISVKSEDYYGSNVLNFKQRNKAFKVFLLGDDKNKYKEKTHGLD
                     VFAVPELIDIKGGIYSVGGITKKNVRSVFGFVSNPSLQVKKVDAKNGFSINELFFIQK
                     EEVSLKELDFKIRKLLIEKYRLYKGTSDKGRIVINMKDEKKHEIDLSEKLSFERMFDV
                     MDSKQIKNIEVNLN"
     gene            complement(2957..3655)
                     /locus_tag="O2701_001962"
     CDS             complement(2957..3655)
                     /locus_tag="O2701_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL8"
                     /protein_id="HCZ4293845.1"
                     /translation="MKFTVIAKAIFILGILTTSVMITENQSVNAKGKYEKMNRLYDTN
                     KLHQYYSGPSYELTNVSGQSQGYYDSNVLLFNQQNQKFQVFLLGKDENKYKEKTHGLD
                     VFAVPELVDLDGRIFSVSGVTKKNVKSIFESLRTPNLLVKKIDDKDGFSIDEFFFIQK
                     EEVSLKELDFKIRKLLIKKYKLYEGSADKGRIVINMKDENKYEIDLSDKLDFERMADV
                     INSEQIKNIEVNLK"
     gene            complement(3999..4694)
                     /locus_tag="O2701_001963"
     CDS             complement(3999..4694)
                     /locus_tag="O2701_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL7"
                     /protein_id="HCZ4293846.1"
                     /translation="MKLKTLAKATLALGLLTTGVITSEGQAVQAKEKQERVQHLYDIK
                     DLHRYYSSESFEFSNISGKVENYNGSNVVRFNQENQNHQLFLLGKDKEKYKEGIEGKD
                     VFVVKELIDPNGRLSTVGGVTKKNNKSSETNTHLFVNKVYGGNLDASIDSFSINKEEV
                     SLKELDFKIRQHLVKNYGLYKGTTKYGKITINLKDGEKQEIDLGDKLQFERMGDVLNS
                     KDINKIEVTLKQI"
     gene            complement(5115..5810)
                     /locus_tag="O2701_001964"
     CDS             complement(5115..5810)
                     /locus_tag="O2701_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL6"
                     /protein_id="HCZ4293847.1"
                     /translation="MKLKTLAKATLVLGLLATGVITTESQTVKAAESTQGQHNYKSLK
                     YYYSKPSIELKNLDGLYRQKVTDKGVYVWKDRKDYFVGLLGKDIEKYPQGEHDKQDAF
                     LVIEEETVNGRQYSIGGLSKTNSKEFSKEVDVKVTRKIDESSEKSKDSKFKITKEEIS
                     LKELDFKLRKKLMEEEKLYGAVNNRKGKIVVKMEDDKFYTFELTKKLQPHRMGDTIDG
                     TKIKEINVELEYK"
     gene            complement(6256..6960)
                     /locus_tag="O2701_001965"
     CDS             complement(6256..6960)
                     /locus_tag="O2701_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL5"
                     /protein_id="HCZ4293848.1"
                     /translation="MKMTAIAKASLALGILATGTITSLHQTVNASEHKAKYENVTKDI
                     FDLRDYYSGASKELKNVTGYRYSKGGKHYLIFDKNRKFTRVQIFGKDIERFKARKNPG
                     LDIFVVKEAENRNGTVFSYGGVTKKNQDAYYDYINAPRFQIKRDEGDGIATYGRVHYI
                     YKEEISLKELDFKLRQYLIQNFDLYKKFPKDSKIKVIMKDGGYYTFELNKKLQTNRMS
                     DVIDGRNIEKIEANIR"
     gene            complement(7324..8250)
                     /locus_tag="O2701_001966"
     CDS             complement(7324..8250)
                     /locus_tag="O2701_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL4"
                     /protein_id="HCZ4293849.1"
                     /translation="MKITTIAKTSLALGLLTTGVITTTTQAANATTPSSTKVEAPQST
                     PPSTKIEAPQSKPNATTPPSTKVEAPQQTANATTPPSTKVTTPPSTNTPQPMQSTKSD
                     TPQSPTTKQVPTEINPKFKDLRAYYTKPSLEFKNEIGIILKKWTTIRFMNVVPDYFIY
                     KIALVGKDDKKYGEGVHRNVDVFVVLEENNYNLEKYSVGGITKSNSKKVDHKAGVRIT
                     KEDNKGTISHDVSEFKITKEQISLKELDFKLRKQLIEKNNLYGNVGSGKIVIKMKNGG
                     KYTFELHKKLQENRMADVIDGTNIDNIEVNIK"
     gene            complement(8359..8496)
                     /locus_tag="O2701_001967"
     CDS             complement(8359..8496)
                     /locus_tag="O2701_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293850.1"
                     /translation="MFVKVAFLCLKSDETSNVPSVESHQNHFYLTNIMDFLIYLTMIQ
                     I"
     gene            complement(8615..9673)
                     /locus_tag="O2701_001968"
     CDS             complement(8615..9673)
                     /locus_tag="O2701_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL3"
                     /protein_id="HCZ4293851.1"
                     /translation="MKMRTIAKTSLALGLLTTGAITVTTQSVKAEKIQSTKVDKVPTL
                     KAERLAMINITAGANSATTQAANTRQERTPKLEKAPNTNEEKTSASKIEKISQPKQEE
                     QKTLNISATPAPKQEQSQTTTESTTPKTKVTTPPSTNTPQPMQSTKSDTPQSPTIKQA
                     QTDMTPKYEDLRAYYTKPSFEFEKQFGFMLKPWTTVRFMNVIPNRFIYKIALVGKDEK
                     KYKDGPYDNIDVFIVLEDNKYQLKKYSVGGITKTNSKKVNHKVELSITKKDNQGMISR
                     DVSEYMITKEEISLKELDFKLRKQLIEKHNLYGNMGSGTIVIKMKNGGKYTFELHKKL
                     QEHRMAGTNIDNIEVNIK"
     gene            complement(9964..10659)
                     /locus_tag="O2701_001969"
     CDS             complement(9964..10659)
                     /locus_tag="O2701_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL2"
                     /protein_id="HCZ4293852.1"
                     /translation="MKMKNIAKISLLLGILATGVNTTTEKPVHAEKKPIVISENSKKL
                     KAYYNQPSIEYKNVTGYISFIQPSIKFMNIIDGNSVNNIALIGKDKQHYHTGVHRNLN
                     IFYVNEDKRFEGAKYSIGGITSANDKAVDLIAEARVIKEDHTGEYDYDFFPFKIDKEA
                     MSLKEIDFKLRKYLIDNYGLYGEMSTGKITVKKKYYGKYTFELDKKLQEDRMSDVINV
                     TDIDRIEIKVIKA"
     gene            complement(10945..11625)
                     /locus_tag="O2701_001970"
     CDS             complement(10945..11625)
                     /locus_tag="O2701_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL1"
                     /protein_id="HCZ4293853.1"
                     /translation="MKFKAIAKASLALGMLATGVITSNVQSVQAKAEVKQQSESELKH
                     YYNKPILERKNVTGFKYTDEGKHYLEVTVGQQHSRITLLGSDKDKFKDGENSNIDVFI
                     LREGDSRQATNYSIGGVTKSNSVQYIDYINTPILEIKKDNEDVLKDFYYISKEDISLK
                     ELDYRLRERAIKQHGLYSNGLKQGQITITMNDGTTHTIDLSQKLEKERMGESIDGTKI
                     NKILVEMK"
     gene            12097..12942
                     /locus_tag="O2701_001971"
     CDS             12097..12942
                     /locus_tag="O2701_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCZ4293854.1"
                     /translation="MNIMLTGATGHLGTHITNQAIANHIDHFHIGVRNVEKVPEDWRG
                     KVPVRQLDYFNQESMVEAFKGMDTVVFIPSIIHPSFKRIPEVENLVYAAKQSGVAHII
                     FIGYYADQHNNPFHMSPYFGYAARLLATSGIDYTYVRMAMYMDPLKPYLPELMNMHKL
                     IYPAGDGRINYITRNDIARGVIAIIKNPDTWGKRYLLSGYSYDMKELAAILSEASGTE
                     IKYEPVSLETFAEMYDEPKGFGALLASMYDAGARGLLDQESNDFQQLVNDQPQTLQSF
                     LQENI"
     gene            12961..13320
                     /locus_tag="O2701_001972"
     CDS             12961..13320
                     /locus_tag="O2701_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HCZ4293855.1"
                     /translation="MNIISTILIIFVALEFFYIMYLETIATTSKKTSETFNISVDKLK
                     DKNINLLLKNQGVYNGLIGVLLIYGLFISSNPKEICAAILVYIIGVAIYGGLSSNISI
                     FFKQGTLPVLALISMLW"
     gene            13844..13996
                     /locus_tag="O2701_001973"
     CDS             13844..13996
                     /locus_tag="O2701_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293856.1"
                     /translation="MVPEEKGSITLSKEAAIIFAIAKFKPFKNRIKNNPQKTNPFLKL
                     HENKKS"
     gene            14257..>14466
                     /locus_tag="O2701_001974"
     CDS             14257..>14466
                     /locus_tag="O2701_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCZ4293857.1"
                     /translation="MTKERRTFSSEFKLQMVRLYKNGKLKNEIIRKYDLKPSIISNSI
                     KQHQNTGPFNHQDNLKSDEKELIKLR"
BASE COUNT         4381 a         2496 c         1961 g         5628 t
ORIGIN      
        1 attaggaatt catatgcatc acctaacata tcaatttcca tgtcactgtg aacgaatggt
       61 aagtcgtcaa gattaaccat gactttagag attaaagcag tacgttcttt gacattgtta
      121 cctagtcgcg ttgaactcaa atccatatcg ctgaacagac cgataaagtc attttcactt
      181 tcttcaccta atgttgatgt ttcaacttta cgaattgccg tcgccaggtg ttcgatatcg
      241 aaatcttgcg tttcaatttc acgaatcatc gcactgaata aatcttctgg ctcaatgaag
      301 taaccgactt ggtcaattaa ttctgctttt aagtcttcac ggtattcttc gtctgcccat
      361 gcttcttgat acgtgatgtc ttcacctgac aaggcatctg catattcttg ttccgctttt
      421 tcagataaga agcgatagaa aatcaagcct aaaatgtaat tacggaattc actcgcatcc
      481 atattccctc ttaaatcatt cgcaatcgac cataattttt tatgtaattc agcttgttgc
      541 tgacgttgtt tttcagtaat agacatgtga ttcctccgcc tttgaataag taatttatct
      601 ctttgtgtaa tagatttatt ataacatttg gttatgttgc gatgtggaga attttgatgt
      661 tggtggtgga aattttacct ttatggagat tgggatagga ttcatgacac acaaaccaaa
      721 acggcttaac gacgatatcg attgatgcat cctttttgta taagctatgc gcaccaattt
      781 acatttcacc gttaagccgc ttcaattact tttaattatt cgtaatgatt tactttaagt
      841 taacttcaat atctttaatt tgtttgcttt ctatgacttc ccccatatat ttaaatttta
      901 atttagatct taaatcaagg ttataaaaac tgccatcttt caagctaatt ttgaccctac
      961 catcttttga gattgtttta taaagtccgt atttttcaat tagatgcttt ctcaacttat
     1021 aatccagttc ttttagtgtt attttttctt ttttaatgta ataagggtaa cctttcacaa
     1081 aagcatcttc acccttttct tttgaaatat ttaatattgg tgcactgaca actccagatg
     1141 ttttattatt ctgtattaca ccaccaacag tataaaatat atcgtgctta tctctttttt
     1201 cttgaacaaa gaaaacatct aacccctcat aactacgctg ttgaaattta cttttatcat
     1261 ttccaggcag taaaacttga atcttaatac ctctaaactt aaaacgtaag ttgtttttac
     1321 catgtttcaa agcactaata tttttcattt ccatagtctt tccagtgtag tatcggtata
     1381 atgcttcctt gtcatgttta tttactgact tttgattttg ttttgcatga cctgaatgaa
     1441 cttctgttgt taaagttcct gttgttaaaa ttcctaaagc taatgtcgct tttgctaatg
     1501 ctgtaaattt catatttatt tgctccaatc ttaatatatt ggattgtttt cattacgtaa
     1561 tttgaatcat acatttttat tataacgtgc atcgtttcat acgataatta acctcgattt
     1621 aaatatactt gaattattct taatgaaaaa caaatatatt ttaatgttca ggttaaatga
     1681 ctttcattat ttcatttagt tgttttaccg gattatcgtt tactacaact gatgatgtta
     1741 ctctgttctg ctctttgaat tacaaaaaca acttaacaac agtaatgtat ttaacacagt
     1801 tccttatata aggacacaaa tgccaattca cattttgtcg ttaagccgct tcttaagcta
     1861 ttatgctatt aactcgaact aattcaaatt cacttcaata tttttaattt gcttactatc
     1921 cattacatca aacatacgtt caaaacttaa tttttcactt aaatcaattt catgcttctt
     1981 ttcgtctttc atattgataa caattctacc tttatcagac gttcctttat acaatctata
     2041 tttttcgatt aagagttttc ttattttaaa gtccagttcc ttcaatgata cttcttcctt
     2101 ttgaataaaa aacaactcgt ttatcgaaaa gccattttta gcatcaactt ttttaacttg
     2161 tagacttgga ttacttacaa atccaaacac tgatctcaca tttttctttg ttataccgcc
     2221 aacgctatat atgccacctt ttatatctat taattcaggt actgcaaaga catcaaggcc
     2281 atgtgttttt tctttatatt tatttttatc gtcaccaagt aaaaatactt taaaagcttt
     2341 atttcgttgt ttaaagttta aaacgttaga gccatagtaa tcttcgcttt taacactaat
     2401 gtttgttggt tcaaaacttt cttcagaata gtattgatgt agcatattga tataatattt
     2461 gcgttgtgtc tcatcaagtt ctactttcgc gtgaccagtt tgactttctg ctgtaaacac
     2521 acctgtagtt aatattccta atgctaatgt tgctttagct atcgttgtta atttcatatt
     2581 tttttgctcc aatcttaatg tattggattg ttattattac gtaatttgaa ctatctgtct
     2641 ttattataag gtgcatcaaa acagactgac attaacgctc atttaaataa acttgaatca
     2701 ttattaatga aaatcaaacg tattttaaca ttcgtattaa ctgattttta atagcaacat
     2761 aaatattttt ttggtgaatc attgttcttt ccgattagtt ttaaatctta agctattggt
     2821 tataaagtgt acaaaaaagc agcttaataa cgtagtcaat gttcgatatg tttttattat
     2881 aaaaacacgt atcaaaatca ttaaacatgg gattattaaa ccgcttctta agctttcatt
     2941 ctatatatat cattgattat ttcaaattca cttcgatgtt tttaatttgt tcactattaa
     3001 tgacatctgc catacgctcg aaatctaatt tatcacttaa atcaatttca tacttatttt
     3061 catctttcat attaataaca attctacctt tatcagctga cccttcatac agtttgtatt
     3121 ttttaatcaa cagttttctt attttaaaat caagttcctt caatgacact tcttcctttt
     3181 gaataaagaa aaattcatca atagaaaaac cgtctttatc gtctattttt ttaactagta
     3241 agttcggcgt tcttagagac tcaaatattg attttacgtt tttctttgtt acaccactaa
     3301 cactaaatat tcttccatct aaatctacta attctggtac cgcaaagaca tctaaaccat
     3361 gtgttttttc tttgtattta ttttcatctt ttcccaataa aaacacttgg aacttttgat
     3421 tttgttggtt aaaaagcaaa acgttagagt cataataacc ttgactttgg ccactaacat
     3481 ttgttaactc ataactaggt cctgaatagt attgatgtaa cttgtttgta tcatataaac
     3541 ggttcatttt ttcatacttt ccttttgcat taaccgattg attttctgtt atcattacac
     3601 ttgttgttaa tattcctaat ataaatatcg ctttagctat cactgtaaat ttcatattca
     3661 tgtgctccaa tcttaatata ttggattgct tttattacgt aatttgaata atacaccttt
     3721 attataaagg gcattgagac agactgacat taacactcgt ttaattaaac ttaaatcatt
     3781 gttaatgaag agctaattaa tattcaatgg gcatcacttt caagagcaat gtaaattttt
     3841 agtatgctca ctcataacct gcgttataaa acgtaaaaaa aagcctaacg acaaggttgt
     3901 tttaaatacg ctccttaatg taaggtgtac gtatctattc aacatttcat cgttaagctg
     3961 cttcaaattt attactttag agtcattgat tacttacttt aaatttgttt caaagtcact
     4021 tcaatcttat taatatcctt actattcaac acatcaccca tgcgctcgaa ttgcaattta
     4081 tcacctaaat caatttcttg cttttctcca tctttcaaat tgatagtgat cttaccgtat
     4141 ttagtcgtac ctttatataa accataattt ttaactaaat gttgtctaat tttgaaatca
     4201 agttctttca gtgaaacttc ttctttatta attgaaaatg agtcaattga tgcatctaaa
     4261 tttccgccat acactttatt aacaaataaa tgtgtattag tttcagaaga tttgttattt
     4321 ttcttagtca caccaccaac agtagataat ctaccgtttg gatcaattaa ttcttttacc
     4381 acaaagacat ctttgccttc aatgccttct ttatatttct ctttatcttt acctaataag
     4441 aataattggt gattttgatt ttcttggtta aagcgtacaa cgttagaacc gttataattt
     4501 tcaaccttac cactaatatt actgaattca aaactttctg atgagtagta tcgatgtaag
     4561 tctttaatat catataaatg ttgtactctc tcttgctttt cttttgcttg aactgcttgg
     4621 ccttctgatg taatcacacc agtagttaat aagcctaatg ccaatgttgc tttagctaac
     4681 gtttttaatt tcacagtact attctcccaa tctatttata aatttgtctt aatatatttt
     4741 tatatgatta attcaattac gtaatacgaa caatctacta tcattatata agtgagatac
     4801 tgcgctatga attaacctct ttttaactat ttttgaacca acgttaagtt agttaatgaa
     4861 atcctaacag tattaattat tttttataac acaatactaa ttttttaact attcacactt
     4921 tccctcaatt taattatgtt ttccacattg tttcatgtca cgaaaaggac aacgcgcgac
     4981 tataagtatc aaccgttttt ccaaaaactt gatcataaac ccgctccttt tttcatcata
     5041 acaaaataaa aaagaagcta agcaatatgt tatcgcttaa cttccctaca attactagtc
     5101 tgcttgtcca aagattattt atattctagc tcaacattaa tttctttgat tttggtacca
     5161 tctatcgtgt cacccatgcg atgcggttgt agtttttttg taagttcgaa agtataaaac
     5221 ttatcatctt ccattttaac tacaatttta ccttttctat tattaacagc accatataat
     5281 ttttcttctt ccatcaattt ttttcttaat ttaaagtcca actcttttaa cgagatttct
     5341 tctttagtaa ttttaaattt actatcttta gacttttccg atgattcatc aatttttctt
     5401 gttactttaa catcgacttc tttactaaat tctttactat ttgtcttact taaaccacca
     5461 attgaatatt gtcttccatt aacagtttcc tcctcgatga ctaaaaatgc atcttgctta
     5521 tcatgctcac cttgagggta tttttcaata tctttaccaa gcaagccaac aaaataatct
     5581 tttcgatcct tccaaacata tactccttta tctgtcactt tctgtctata caaaccatca
     5641 agatttttta actctatact tggcttgcta tagtagtatt ttaacgattt ataattgtgt
     5701 tgaccttgag ttgattctgc cgcttttact gtttgacttt ctgttgttat tacaccagta
     5761 gctaacaatc ccaatactaa tgttgcttta gctaacgttt ttaatttcat agtactattc
     5821 tcccaatcta tttataaatt ttgtcttaat atatttttat atgattattt caattacgta
     5881 atacgaacaa tctactatca ttatataagc gtaatactaa gctatgaatt aacctctttt
     5941 taactatttt tgaaccaatg ttaagctaat taatggaatc ctaacagcgt taatctattt
     6001 tttaaactta acgcagcttt tttaactatt cacactttcc ctcaatttaa ctatgttttc
     6061 cacattgttt catgtcacga aaaggacaac gcgcgactat aagtatcaac tatttccaca
     6121 agttttattg gtgtttttat tattcatcga tacgcttcat tttcatctct ccaacacaaa
     6181 aaagaagcta agcaacttat gttgcctaac tcctctatac tatccatatt ttactattat
     6241 ccatatttca ttgaattatc taatgttggc ttctattttt tcaatatttc taccgtcaat
     6301 gacgtcactc atgcgatttg tttgtaattt tttattaagt tcaaacgtat aatagccgcc
     6361 atctttcatt atcactttta tcttactatc tttaggaaac tttttataca gatcaaaatt
     6421 ttgaattaaa tactgtctca atttaaagtc gagttcttta agtgaaatct cttctttata
     6481 aatgtagtgt actctaccgt acgtagcaat accgtcacct tcatctctct tgatttgaaa
     6541 tcttggtgcg tttatataat cataataagc gtcttgattt ttcttagtga caccaccata
     6601 tgaaaacact gtgccattac ggttttccgc ttctttaaca acaaatatgt ctaatcccgg
     6661 atttttacgt gctttaaatc tttcaatatc tttaccaaat atctgtactc ttgtgaattt
     6721 tctattttta tcaaagataa ggtaatgctt gccacctttg ctataacgat aaccagtaac
     6781 atttttaagt tccttacttg cgccactata gtaatctctt aagtcaaaga tatcttttgt
     6841 cacattttca tattttgctt tatgttcact cgcatttaca gtttgatgca atgacgttat
     6901 tgttcctgtt gctaaaatac ctaatgctaa acttgctttc gcaattgctg tcattttcat
     6961 agttgtatgc tccattcgta attattagat ttgttcgctt acgtctattg aatcatacag
     7021 ctttattata gttagcgtat ttgacctttc acattaaacc atgtttaata atcattgaat
     7081 cattattaag taaattaagg aatctataat gttcgttaaa taaaactgat cccgttgtgc
     7141 ttcacacccg atagataggg atttacagat aaattcaggt ctcttccacg tcatatttgg
     7201 acccatcgaa aattcgggtt ctcaaatcat cgaacataac aaaagaagct aagcaacatg
     7261 taggccgttg tcacttaact tcttgttttt ccgatgacag cttctattta gagaatgtca
     7321 tgattatttt atattcactt caatgttatc aatattagtg ccatctatga catctgccat
     7381 gcgattttct tgtaattttt tgtgcaattc aaacgtgtac tttccaccgt ttttcatttt
     7441 aataacaatt ttacctgaac caacgttacc gtacagatta tttttttcaa taagttgttt
     7501 tctcaattta aaatcaagtt ctttcaagga aatctgttct ttagtaatct tgaattctga
     7561 aacatcatga gagattgtac ctttattatc ttccttagta attcttactc ctgctttgtg
     7621 atcaactttt ttactattac tctttgtgat accaccgaca gaatattttt ccagattgta
     7681 attattttct tctaaaacga caaatacatc gacattccta tgtactcctt caccatattt
     7741 tttatcatct ttaccaacta aagcaatttt atatatgaaa taatctggga caacattcat
     7801 aaatcttatt gtcgtccatt tttttaaaat aataccaatc tcatttttaa attctaaact
     7861 tggtttcgta taatacgctc ttaaatcttt aaatttagga tttatttctg ttggtacttg
     7921 ttttgtggtt ggcgattgtg gtgtgtctga tttagtagat tgcattggtt gtggcgtgtt
     7981 tgttgatgga ggtgttgtca ctttagttga aggcggtgtt gtcgcatttg ctgtttgttg
     8041 cggtgcttct actttagttg agggcggtgt tgtcgcgttt ggttttgatt gcggtgcttc
     8101 tattttagtt gagggcggtg ttgattgtgg tgcttccact ttagtggaag atggtgttgt
     8161 cgcgtttgct gcttgcgttg tcgttgtgat tacacctgtt gttaaaaggc ctagtgctaa
     8221 acttgtttta gcaatcgttg ttattttcat agttgtatgc tccattcgta attattagat
     8281 ttgttcgatt acattcattg aatcatacag ctttattata gatggcgtat tgctccattc
     8341 acattaaacc ttgtttaact atatttgaat catcgttaag taaattaaga aatccataat
     8401 gttcgttaaa taaaaatgat tttgatgtga ttcaacactt ggcacatttg aagtttcgtc
     8461 acttttaaga catagaaatg ccacttttac aaacaaatga atattcgtct ttttacatca
     8521 ttacgcataa taaaagaagc taagcaacat gtaaaccgtt gtcacttaac ttcttgtttt
     8581 tccgatgaca gcttctattt agagaatgtc atgattattt tatattcact tcaatgttat
     8641 caatattagt gcctgccata cgatgctctt gcagtttttt gtgtaattca aacgtatatt
     8701 tcccaccgtt tttcatttta ataacgattg ttcctgaacc catgttaccg taaagattat
     8761 gtttttcaat aagttgtttt ctcaatttaa aatcaagctc tttcaaggaa atctcttcct
     8821 tagtaatcat gtattctgaa acatcgcgtg aaatcatacc ttgattatct tttttagtaa
     8881 tgcttaattc tactttgtga ttaacttttt tactattagt cttcgtgatg ccaccgacag
     8941 aatatttttt caattgatat ttattgtctt ctaaaacgat aaatacatcg atattatcgt
     9001 aaggtccatc tttatatttt ttctcatctt ttccaactaa agctatttta tagatgaacc
     9061 tatttggaat aacattcata aacctaaccg tcgtccatgg tttgagcata aatccaaact
     9121 gcttttcaaa ttcaaaactc ggttttgtat aatacgctct taaatcttca tatttaggag
     9181 tcatatctgt ttgtgcttgt tttatggttg gagattgtgg tgtgtctgat ttagtagatt
     9241 gcattggttg tggcgtgttt gttgatggag gtgttgtcac tttagttttc ggcgttgtgg
     9301 attcggttgt cgtttgtgat tgttcttgtt taggcgctgg cgttgctgat atattaagcg
     9361 ttttctgctc ttcttgttta ggttgtgata ttttttctat tttggaagct gaggtttttt
     9421 cctcattagt atttggtgcc ttttcgagtt taggcgtgcg ttcttgtctt gtgttagctg
     9481 cttgtgttgt cgctgaattt gcacctgctg ttatgtttat cattgctaat cgctctgctt
     9541 taagcgttgg tactttgtca actttagttg attgtatttt ttctgctttg accgattgcg
     9601 tcgttactgt aattgcgcct gttgttaaaa gccctagtgc taaactggtt ttagcaattg
     9661 ttctcatttt cataattgta tgctccaatc tatattatat tcgattgtct ttttacgtaa
     9721 tttgaatcat acaacatcat tatagatggc gttctaagat aatcacatta aacccctttt
     9781 aacaattatt gaagtattat taagtaattt aagcaaaaaa taatgagtga gtatgagatt
     9841 aatatagcgt ttctatgtgc ctttgaaata atttttaagc attaaaaaga agttaagcaa
     9901 cgtttgatcg tcacttaacc tctctatttc aatttcaact tatttcgtca tcaagtatat
     9961 gtgttatgct tttataactt tgatttcaat tctatcaata tctgtgacat tgataacatc
    10021 ggacatacgg tcttcttgta actttttatc caattcaaat gtatactttc catagtattt
    10081 ctttttgact gtaatttttc ctgtactcat ttcaccgtaa agaccataat tatcaataag
    10141 gtattttctt aatttaaaat caatctcttt caatgacatc gcttctttat ctattttaaa
    10201 tgggaaaaag tcataatcat attcaccagt atgatcttct ttaataactc ttgcttctgc
    10261 tattaggtcg acagctttat cgtttgcact cgtgataccc ccaatagagt actttgcacc
    10321 ttcaaatctc ttatcctcat taacgtaaaa tatattaaga ttacgatgta cacccgtatg
    10381 ataatgttgc ttatctttgc caattaaagc aatattatta acagaattac catctatgat
    10441 attcataaat ttaatacttg gttgaatgaa actgatataa cctgtcacat ttttatattc
    10501 aatactaggt tgattataat aagcttttaa ttttttgcta ttttcactta ttacaatagg
    10561 tttcttttcg gcatgaactg gtttttccgt tgtagtgttt acacctgttg ctaatattcc
    10621 taataacaaa cttatttttg caatattttt cattttcata gttgtatgct ccaatctatt
    10681 ataattagat tgttttatta cgtaatttga atcatacacc catattatag gagctgtatt
    10741 cggatattca cattaacctg tttttaacta ttcataaaat atgattaagc tatttaagca
    10801 aaagataatg cgtggtgcga ctgtttgtgt gagttttgta acttttcccc tcaaaaaaag
    10861 aagctaagca acagtacgtc gcttaacttc catatatttc cataacaagc actattcaac
    10921 atagcgcttt gttgttagaa agtattattt catttctact agaattttat taatcttagt
    10981 gccgtcgatt gactcaccca tacgttcttt ttcaagtttt tgacttaaat cgattgtatg
    11041 tgttgtgcca tcattcattg taattgtaat ttgaccttgt ttaagaccat ttgaatacaa
    11101 gccgtgttgt ttaatcgcac gttctcttaa tctataatca agttctttta atgagatgtc
    11161 ttcttttgaa atgtagtaaa aatctttaag tacatcttca ttatctttct tgatttctaa
    11221 aattggcgta ttgatataat caatatactg cacactattt gattttgtaa cgccaccaat
    11281 tgagtaattt gttgcttgtc tactgtcacc ttctctaagg ataaacacat ctatatttga
    11341 gttttctccg tctttaaatt tatctttatc agatccaagt aaagtgattc gagaatgctg
    11401 ttgccctact gtgacttcta aatagtgttt accctcatca gtatatttaa atccagtcac
    11461 atttttacgc tctaaaattg gtttattata atagtgtttt aactctgatt cactttgttg
    11521 tttaacttct gctttcgctt gtactgattg tacattcgat gtaattacac ctgttgctaa
    11581 cattcccaat gctaaacttg cttttgctat cgctttaaat ttcataattt ttagccccaa
    11641 tctatttaaa tttgattttt caattacgta atatgaacaa tctacctaca ttatattgag
    11701 gcgctatccc ctttcaaatt aaacctcatt taactaaact tgaatcattg ttaaggtaat
    11761 taataaaatg ctaactgtta ttaatcaatt cttatccgtc gagactcttt tttatacaaa
    11821 tcacacttta taccattttt attttaaaaa ctgtaaaact ttcagacaca aaacagacat
    11881 catattgtcg tagtacctga ttaaaatttt caatgatatt agtgatacac atcaactaaa
    11941 acgcgtcatt acggcatttt caacattaat aatatttacg tatttactat acacgtcgac
    12001 aataaataca gattagaatt ttttataaaa tcaaatgttt tgaaaaccat caaataagta
    12061 ttatgttatg tatataaaga ttaggagatg aagtgaatga atattatgtt aacaggtgct
    12121 acaggtcatt taggcacaca tattacaaat caagccattg caaatcacat agatcatttt
    12181 cacattggtg ttagaaatgt tgagaaagtt ccagaagatt ggcgcggaaa agttcctgtt
    12241 cgacaattag attattttaa tcaagaaagc atggtagaag catttaaagg aatggatacc
    12301 gttgtgttta ttccaagtat tatccatcca tcattcaaac gtattcctga agtggaaaat
    12361 ttagtatatg cggcaaagca gagcggcgtt gctcatatca ttttcatagg ttattacgca
    12421 gatcagcata ataatccatt ccatatgagt ccttattttg gttatgcagc acgtctattg
    12481 gcaacaagtg gcattgacta tacgtatgta agaatggcaa tgtacatgga tccacttaaa
    12541 ccatatttac cagaattgat gaatatgcat aaactgattt atccagctgg cgatggtcgt
    12601 attaattata ttactagaaa tgatattgct agaggtgtca ttgctattat taaaaatcca
    12661 gatacttggg gcaaacgcta cttattatca ggctacagtt atgatatgaa agaacttgct
    12721 gcaattttat ctgaggcatc aggcacagaa attaaatatg agcccgtttc attagagaca
    12781 tttgcagaaa tgtatgatga acctaaaggc tttggtgcat tattggcatc aatgtacgac
    12841 gcaggagcaa gaggactatt agaccaagaa tccaatgatt tccaacaatt agtcaatgat
    12901 caaccacaga cactgcaatc atttttacaa gaaaatattt aataataaag gagcgttata
    12961 gtgaatatca tctcaacaat tttaatcata tttgtggcat tagagttttt ctatattatg
    13021 taccttgaaa cgattgctac aacttccaaa aagactagcg agacatttaa tataagcgtc
    13081 gataaattga aagacaaaaa tattaaccta cttttgaaga accaaggcgt atataacggt
    13141 ttaatcggag ttttgctaat atacggtttg tttatcagca gtaatccaaa agaaatatgc
    13201 gcagctattt tagtgtatat cattggcgtt gctatttatg gtggcctttc aagcaatatt
    13261 agtatctttt tcaaacaagg cacattgcca gtattggcac tcatatcaat gctttggtaa
    13321 gtattggtgt ttgggggggt ggagatgtag tcggaggttt ggaggatttg agcgaattgt
    13381 gtgtggactt tagactcaga gtatttcatc ctaattattt caagcagagg tgacagtagc
    13441 gttgcctctg tttccttata gaaaaattat ttaatgaaga aaacccatat ctgatttaat
    13501 tttcagctga taaatactcc atatattaga atggctactt tatctattgc atcaatcctt
    13561 taaaacaaaa aacccatgat ttcgaaattc ccgtatgatg gggttcctac tctcatggat
    13621 cagttaaata aatattatca ctatcagttt attatttcaa tattattaac aatatatgta
    13681 gtcgtaaaag gaaagaggac atgagaactt cggtgttgat tggcattaca taacgcttcc
    13741 aaacatatta tttggtaaca ataagaaact atttacacaa tatattttgt atagtaaaat
    13801 tattttataa tatttaaatc caattgcaca aggagtgatt atcatggtac cagaagaaaa
    13861 aggttctatt actttgtcaa aagaagcagc tatcatattt gcaatcgcaa aattcaaacc
    13921 atttaagaac agaattaaaa ataacccaca aaaaacaaat ccatttctta aattacatga
    13981 aaacaaaaaa tcttaatcac tttatttata gcatttctaa tctcagaaat gctataaagg
    14041 acaacgccct ctttcataac tatataagtc aagaaaccaa gacatttcaa acatctttaa
    14101 ccccttcaat agcacattcc ctaaaacata aagcaataac aatgaacgat caaaattcct
    14161 tacgtactta tgatgtgtca gtgaatcgtc tacacttaat tgaacaaatt ctatgagaat
    14221 agatattgtt aaattaagaa agtagtcaat tttattatga caaaagaaag aagaacattt
    14281 agttcagagt ttaagttaca aatggttaga ttatataaaa atggtaagct taagaatgaa
    14341 attatacgaa agtatgattt aaaaccctca attatctcaa attcgataaa acaacaccaa
    14401 aatactggac ccttcaatca tcaagataat ttaaaaagtg atgaaaaaga gttaataaaa
    14461 ttacgc
//
LOCUS       DALAGD010000076        14439 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.077, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000076 DALAGD010000000
VERSION     DALAGD010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14439
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.077"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..113
                     /locus_tag="O2701_001975"
     CDS             <1..113
                     /locus_tag="O2701_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3885 domain-containing protein"
                     /protein_id="HCZ4293858.1"
                     /translation="LYDDRGLWLAFNNNEDYARYSEKYNDLISEFDNEDI"
     gene            complement(206..367)
                     /locus_tag="O2701_001976"
     CDS             complement(206..367)
                     /locus_tag="O2701_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293859.1"
                     /translation="MLLILELRFWNLEFGFRNSTFGIQNYNEWKILIIACLMTWFCSD
                     FYLMNCFFV"
     gene            complement(404..1144)
                     /locus_tag="O2701_001977"
     CDS             complement(404..1144)
                     /locus_tag="O2701_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCZ4293860.1"
                     /translation="MFNLKHDLETLKHIIDSSNRITFFTGAGVSVASGVPDFRSMGGL
                     FDEISKDGLSPEYLLSRDYLEDDPEGFINFCHKRLLFVDTMPNIVHDWIAKLERNQQS
                     LGVITQNIDGLHSDAGSQHVDELHGTLNRFYCNVCHKSYTKSDVIDRTLKHCDNCGGA
                     IRPDIVLYGEMLDQPTIIRALNKIEHADTLVVLGSSLVVQPAAGLISHFKGDNLIIIN
                     KDRTPYDSDATLVIHDDMVSVVKSLMTE"
     gene            1350..2141
                     /locus_tag="O2701_001978"
     CDS             1350..2141
                     /locus_tag="O2701_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCZ4293861.1"
                     /translation="MTHRLNKGGNFTLNKHERLDEIAKLVNKKGTIRTNEIVEGLNVS
                     DMTVRRDLIELENKGILTKIHGGARSNSTFQYKEISHKEKHTRQIAEKRFIAKKAASL
                     IEDGDTLFFGPGTTVELLAEEVNHHTLTIITNCLPVYKILLEKQTAHFRVYLIGGEMR
                     HITEAFVGEMANAMLEKLRFSKMFFSSNAVNKGAVMTSTLDEAYTQQLALSNSIEKYL
                     LIDHTKVGKEDFTSFCQLNELTAVVMDYEDEEKVETIKTYIEVVD"
     gene            2509..2937
                     /gene="lacA"
                     /locus_tag="O2701_001979"
     CDS             2509..2937
                     /gene="lacA"
                     /locus_tag="O2701_001979"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HCZ4293862.1"
                     /translation="MAIIIGSDEAGKRLKEVIKSYLLDNKYDVVDVTEGQEVDFVDAT
                     LAVAKDVQSQEGNLGIVIDAFGAGSFMVATKIKGMIAAEVSDERSGYMTRGHNNSRMI
                     TMGSEIVGDTLAKNVVKGFVEGKYDGGRHQIRVDMLNKMC"
     gene            2953..3468
                     /gene="lacB"
                     /locus_tag="O2701_001980"
     CDS             2953..3468
                     /gene="lacB"
                     /locus_tag="O2701_001980"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HCZ4293863.1"
                     /translation="MKIALGCDHIVTDTKMRVSEFLKSKGHEVIDVGTYDFTRTHYPI
                     FGKKVGEQVVSGNADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSALYAKEELNAN
                     VIGFGGRIIGELLMCDIIDAFINAEYKPTEENKKLIAKIKHLETSNADQADPHFFDEF
                     LEKWDRGEYHD"
     gene            3481..4413
                     /gene="lacC"
                     /locus_tag="O2701_001981"
     CDS             3481..4413
                     /gene="lacC"
                     /locus_tag="O2701_001981"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HCZ4293864.1"
                     /translation="MILTLTLNPSVDISYPLTALKLDDVNRVQEVSKTAGGKGLNVTR
                     VLAQVGEPVLASGFIGGELGQFIAKKLDHADIKHAFYNIKGETRNCIAILHEGQQTEI
                     LEQGPEIDNQEAAGFIKHFEQLLEKVEAVAISGSLPKGLNQDYYAQIIERCQNKGVPV
                     ILDCSGATLQTVLENPYKPTVIKPNISELYQLLNQPLDESLESLKQAVSQPLFEGIEW
                     IIVSLGAQGAFAKHNHTFYRVNIPTISVLNPVGSGDSTVAGITSAILNHENDHDLLKK
                     ANTLGMLNAQEAQTGYVNLNNYDDLFNQIEVLEV"
     gene            4417..5397
                     /gene="lacD"
                     /locus_tag="O2701_001982"
     CDS             4417..5397
                     /gene="lacD"
                     /locus_tag="O2701_001982"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCZ4293865.1"
                     /translation="MSKSNQKIASIEQLSNNEGIISALAFDQRGALKRMMAKHQTEEP
                     TVAQIEQLKVLVAEELTQYASSILLDPEYGLPASDARNKDCGLLLAYEKTGYDVNAKG
                     RLPDCLVEWSAKRLKEQGANAVKFLLYYDVDDAEEINIQKKAYIERIGSECVAEDIPF
                     FLEVLTYDDNIPDNGSVEFAKVKPRKVNEAMKLFSEPRFNVDVLKVEVPVNMKYVEGF
                     AEGEVVYTKEEAAQHFKDQDAATHLPYIYLSAGVSAELFQETLKFAHEAGAKFNGVLC
                     GRATWSGAVQVYIEQGEDAAREWLRTTGFKNIDDLNKVLKDTATSWKQRK"
     gene            5419..5730
                     /locus_tag="O2701_001983"
     CDS             5419..5730
                     /locus_tag="O2701_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCZ4293866.1"
                     /translation="MNREEVQLLGFEIVAFAGDARSKFLEALTAAQAGDFAKADALIE
                     EGNNCIAEAHRAQTSLLAKEAQGDDIAYSVTMMHGQDHLMTTILLKDLMKHLLEFYKR
                     G"
     gene            5736..7448
                     /locus_tag="O2701_001984"
     CDS             5736..7448
                     /locus_tag="O2701_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCZ4293867.1"
                     /translation="MMQKLIAQIEKGKPFFEKLSRNIYLRAIRDGFISAMPVILFSSI
                     FLLIAYVPNIFGFKWDKGMEAILMKPYNYTMGLVAFLVAGTTAKSLTDSFNRKLESTN
                     QINFISTMLAAMCGFLFLASDPAKDGGFLSAFMGTKGLLTAFLSAFVTVIVYNFCVKR
                     NITIKMPKEVPPNISQVFKDLIPFSAVIIILYALDLVIRNSFKSNVAEGILKLFEPLF
                     TAADGWIGVTIIFGAFALFWFVGIHGPSIVEPAIAAITYANIEANFKLLQAGEHADKI
                     ITSGTQMFIVTFGGTGATLVVPFMFMWMTKSKRNKAIGRASVVPTFFGVNEPILFGAP
                     LVLNPVFFIPFVLAPIVNVWIFKLFVEVLGMNSFSVNLPWTTPGPLGIIMGTGFGLWS
                     FVLAITLIVVDIIIYYPFLKVYDSEILDEEEGRKESNSDLKEKVAANFDTKKADSILA
                     ASGVSDDAAKASNITEQTNVLVLCAGGGTSGLLANALNKAAEEYHVPVKAAAGGYGAH
                     MDIMKEYQLIILAPQVASNYEDIKQDTDRLGIKLAKTQGAEYIKLTRDGQAALDFVQQ
                     QFEN"
     gene            7466..8878
                     /gene="lacG"
                     /locus_tag="O2701_001985"
     CDS             7466..8878
                     /gene="lacG"
                     /locus_tag="O2701_001985"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HCZ4293868.1"
                     /translation="MTKTLPEDFIFGGATAAYQAEGATNTDGKGRVAWDTYLEENYWY
                     TAEPASDFYNRYPVDLELSEKFGVNGIRISIAWSRIFPNGYGEVNPKGVEYYHKLFAE
                     CHKRHVEPFVTLHHFDTPEVLHKDGDFLNRKTIDYFVDYAEYCFKEFPEVKYWTTFNE
                     IGPIGDGQYLVGKFPPGIKYDFEKVFQSHHNMMVAHARAVKLFKDGGYKGEIGVVHAL
                     PTKYPFDPSNPEDVRAAELEDIIHNKFILDATYLGKYSRETMEGVQHILSVNGGKLNI
                     TDEDYAILDAAKDLNDFLGINYYMSDWMRGYDGESEITHNATGDKGGSKYQLKGVGQR
                     EFDVDVPRTDWDWMIYPQGLYDQIMRVVKDYPNYHKIYITENGLGYKDEFIESEKTVH
                     DDARIDYVRQHLNVIADAIIDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQE
                     RYPKKSAYWYKELAETKEIK"
     gene            9144..10013
                     /locus_tag="O2701_001986"
     CDS             9144..10013
                     /locus_tag="O2701_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCZ4293869.1"
                     /translation="MKKIFVIITTLLAVAIIIGSIIMVVFSQRQAQTFKIQQQQFVKK
                     PIPTLFLHGFGGSANSEKFMVKQAEKRGVTKDIITAYVSKDGAVTFKGKLRKDAVNPI
                     VKIELENNRQGYLDKNAAWFKNVLTKLQSEYNFDKFNFVGHSMGNLTFAQYMMTYGND
                     KSLPQLNKQVNIAGTFNGVLNMNEDVNEITVDKDGKPSRMNQPYQQLRVLKDIYKGKG
                     IEVLNIYGDLKDGTHSDGRVSNSSSKSLKYLLGNSPKSYRESKYEGEPAQHSQLHENE
                     NVANELIDFLWKK"
     gene            10296..11297
                     /locus_tag="O2701_001987"
     CDS             10296..11297
                     /locus_tag="O2701_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HCZ4293870.1"
                     /translation="MQNKQILFNKIPEGMPQEDTFKIEEIDTPKLESDGVLVQTLYIS
                     VDPYMRGRMTKADSYVQPFEIGKPIVSHVVAKVIDSTLADYKKGDVVVGMLPWRIINH
                     VQADQITKVPTTDVPLDLYLSVLGMPGQTAYHGLLDIGQPKAGDTVVVSAASGAVGSV
                     VGQIAKIKGCRVVGIAGGDKKVNYLTETLGFDAGIDYKKEDFAEALAQAVPDGIDVYF
                     ENVGGTVGDEVFKHLNRFARVPVCGAISSYNHPEADIGPRIQGTLIKKQVMMRGFLVS
                     EFANAFKEASEQLATWVQEGKIQSQVTIEDGFENAPHAFKNLFTGDNFGKQVIKVTE"
     gene            11531..12532
                     /locus_tag="O2701_001988"
     CDS             11531..12532
                     /locus_tag="O2701_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HCZ4293871.1"
                     /translation="MKMIGFEKPFKLEEGNLFKVYEQRKPTPENDDILVKVNSISVNP
                     VDTKQRQMEVTQAPRVLGFDAIGTVEAIGPDVTLFSPGDVVFYAGSPNRQGSNATYQL
                     VSEAIVAKAPHNISANEAVSLPLTGITAYETFFDTFKISTNPAENEGKSVLIINGAGG
                     VGSIATQIAKRYGLTVITTASRQETTEWCEKMGADIVLNHKEDLVRQFKEIPLVDYIF
                     CTYNTDLYYNTMIELIKPLGHITTIVAFNEDQDLNALKLKSITFTHEFMFARPIHRTP
                     DMIKQHEYLEDITKNIESGHYQPTTTQVFEGLSPENLYQAHQLLEKQSMIGKLVINI"
     gene            12839..14401
                     /locus_tag="O2701_001989"
     CDS             12839..14401
                     /locus_tag="O2701_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCZ4293872.1"
                     /translation="MKNKSVSLVFWVSLVICTIFVAFGAIFPKQLEKLTQNITTFIAL
                     HFSWYYLLLVLVILFVCVYILFSRYANITLGEEGEDPEFSLPSWFAMLFSAGMGIGLV
                     FWTTAEPISHAFKLTPIHKAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFH
                     KGYPGLVSATLTPLLGEKAMRGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFL
                     FNVPSNFTMQVILIVIATILFTWSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTL
                     YILNTFTNGLGNYIANFFSMSLRIPTGGQKFQWLQNWTIFYWAWWISWAPFVGIFIAR
                     VSKGRTIKEFILGVLFVPALVCFIFFAVFGASAIYLQDNHIADIAKAATETATFATLQ
                     HYPLGFVLSLITLLVIMIFFVTSADSATYVLGMLSSSGDINPKSFVKVSWGIIMALFA
                     IIMIYTGGTQAIQNLLIIAALPFSVVIIAMIWSLIKSLSEEKPRNSNKVLIKHRDPDI
                     LEYRSSKHIDEN"
BASE COUNT         5040 a         2140 c         2780 g         4479 t
ORIGIN      
        1 atttatatga tgatagaggt ctatggctag cttttaataa taatgaagat tatgcaagat
       61 attctgaaaa atataatgat ttaatatcgg aatttgataa tgaagatatc taatttttta
      121 atattcgtag cttttatttt tctacttatt ttatgtttat ttttaatctt acaaatgaca
      181 aaccattaaa agtagcatcc caacatcaaa caaaaaaaca attcatcaaa taaaaatcgc
      241 tacaaaacca agtcattaaa cacgcaataa ttaaaatttt ccactcatta taattctgaa
      301 ttccaaatgt cgaattccga aaaccaaact ccaaattcca aaaacgcaac tccaaaatta
      361 aaagcatttc cctaccattc gggaaatgct ttttacatac tgattactct gtcattaatg
      421 attttacaac ggaaaccatg tcgtcatgta tgaccaaagt agcgtcgcta tcataaggtg
      481 ttcgatcttt attgataata attaaattgt cgcctttaaa atgtgatatt aatcctgcgg
      541 caggttgtac aacgagtgat gaacctagta caacaagggt gtcagcatgt tcaattttat
      601 ttaatgccct tatgatggta ggttgatcta acatttcacc gtataatacg atgtccggtc
      661 gaatggcacc accacaatta tcacaatgtt tcaaagttct atcaataaca tctgacttcg
      721 tataagattt atgacataca ttacaataaa aacgatttaa cgtgccatgt aattcatcaa
      781 catgttgact tccagcgtct gagtgcaaac catcgatatt ttgcgtgatg acacctaaag
      841 attgttgatt acgttctaat tttgcaatcc aatcatgaac gatattgggc atcgtatcga
      901 caaatagtaa gcgcttatgg cagaaattga taaaaccttc aggatcatct tctaaataat
      961 cacggcttaa caagtattct ggcgaaagcc catcttttga aatttcatca aataagccac
     1021 ccattgaacg gaaatctgga acgccacttg cgacagatac accagcacct gtaaaaaatg
     1081 taatacgatt cgaactatct ataatatgtt ttagtgtctc taaatcatgt ttcaagttaa
     1141 acaccccatc aaaataatga atgatttata acgtctttta cttttattat agtatattca
     1201 ttttacgatt atcaaaagcg cagtttaaac agttcttttt aaaaatgaat tatacatata
     1261 atcgaacagt taaattatat tagtttttac gcaattttta ttatagaata agcatgtata
     1321 ttaaaattga tgtctaaaca taaaggataa tgacccatcg cctaaataaa ggaggcaatt
     1381 ttacattgaa taaacatgaa cgtttggacg aaattgctaa actagtgaat aaaaagggca
     1441 cgataagaac gaatgaaatc gtcgaaggtt taaatgtgtc tgatatgaca gttcgaagag
     1501 atttaattga attggaaaat aaagggattt taacgaagat tcatggtggt gcacgcagta
     1561 attcaacatt tcagtataaa gaaatatcgc ataaagaaaa acatacccgt caaattgcag
     1621 aaaaacgatt tatagctaaa aaagctgcat cattaattga agatggggat actttgtttt
     1681 tcggaccagg aacaacagtg gaactattag cagaagaagt caatcatcat acgctcacaa
     1741 ttattacgaa ttgtttgccg gtgtataaaa ttttgttaga aaaacaaaca gcacattttc
     1801 gtgtctattt aattggtggt gaaatgcgcc atattacaga agcatttgta ggtgaaatgg
     1861 cgaacgccat gttggaaaaa ctaagattta gcaagatgtt ctttagtagt aacgcagtaa
     1921 ataaaggtgc tgtgatgaca tctacattag atgaagccta tacgcaacaa ctggcactaa
     1981 gtaattcaat tgaaaaatac ttgttaatcg atcatacgaa agttggcaaa gaagatttta
     2041 catcattttg tcagttaaac gaattgactg cagtggtcat ggactatgaa gatgaagaaa
     2101 aagtagaaac gattaaaaca tatattgaag tagtcgacta aattaaaagt ataactgcat
     2161 tgttaaaata tgtatctata tgctcataag tgaatgtgaa tttgcattca tttatgggct
     2221 attttttatg caaatttatg tcaacgggaa cctcgacaaa gttttactac aatttcagac
     2281 gaatacctaa ttaaaacaac accacaatat gaatgttcac ttattgtttg aaaatacgtc
     2341 ataaacaaac atattataaa catattatgt tgatttattg tttgtttatg tttataattt
     2401 aaacgtaatc aaattgaaag ccttttctca agattttcat aacctacaaa taaatatgtc
     2461 acattttaag aaaggtattt caaaattaaa gtaaaaagga gtcttattat ggcgattatt
     2521 attggttcag atgaagctgg caaacgatta aaagaagtca tcaaatcata cttattagac
     2581 aataaatatg atgttgttga cgtaacagaa ggacaggaag ttgactttgt tgatgcaact
     2641 ttggctgtag caaaagatgt tcaaagtcaa gaaggtaact taggtattgt tatcgatgca
     2701 tttggtgcag gtagctttat ggttgctaca aaaatcaaag gcatgattgc tgctgaagta
     2761 tcagatgaac gctctggata tatgactaga ggccataaca attcaagaat gatcactatg
     2821 ggatctgaaa ttgttggaga cacattagcg aaaaatgtcg ttaaaggttt tgttgaaggt
     2881 aaatacgatg gtggtagaca ccaaatccgc gtagatatgc ttaataaaat gtgctaagga
     2941 ggaaaaatta ctatgaagat tgcattagga tgcgaccata ttgttacaga tacaaaaatg
     3001 cgtgtatctg aatttttaaa atcaaaagga catgaagtca ttgacgtagg aacatacgat
     3061 ttcacaagaa cacattatcc aatttttggt aaaaaagttg gcgaacaagt tgttagcggt
     3121 aatgcagact taggtgtttg tatttgtgga acaggtgttg gtattaacaa tgctgtaaat
     3181 aaagtacctg gcgttcgttc agcactagta cgtgatatga catcagcgtt atacgctaaa
     3241 gaagaattaa atgcgaacgt tattggcttc ggtggacgta ttataggtga gttattaatg
     3301 tgcgatatta tcgatgcatt tattaatgct gaatataaac caactgaaga gaacaaaaaa
     3361 ttaatcgcta aaattaaaca tttagaaaca agcaatgcag atcaagctga tccacatttc
     3421 tttgatgaat tcttagaaaa atgggacaga ggcgaatacc acgattaagg gacgacacat
     3481 atgattttaa ctttaacatt aaatccatcc gttgatattt catacccatt aacagcattg
     3541 aaattagatg atgttaatcg tgttcaagaa gtaagtaaaa cagctggtgg taaaggttta
     3601 aatgtaacaa gagtacttgc gcaagtaggt gagccagtcc ttgcgagtgg ttttattggc
     3661 ggtgaattag gtcaatttat tgctaaaaaa ttagatcatg ccgacatcaa gcatgcattt
     3721 tataacatta agggtgaaac acgaaactgc attgcaattt tacatgaagg gcaacaaaca
     3781 gaaattttag agcagggacc ggagattgat aatcaagagg ctgcaggatt tattaaacat
     3841 tttgaacaat tattagaaaa agttgaagca gttgctattt caggtagctt acctaaagga
     3901 ttaaatcaag attattatgc acaaatcatt gaacgatgcc aaaacaaagg tgtgcctgtg
     3961 atattagatt gctcgggtgc aactttgcaa acagttttgg aaaatccata caaacctaca
     4021 gtcattaaac caaacatttc tgaactatat caattgctta accagccact tgatgaaagt
     4081 ttagaaagtt tgaaacaggc agtgtcacaa cccttattcg aagggattga gtggattatt
     4141 gtgtctttag gtgcacaagg tgcattcgct aagcacaatc atacattcta tagagtgaat
     4201 attccgacaa taagtgtatt aaatccggtt ggttcaggtg attctacagt tgctggaatt
     4261 acttcagcaa ttcttaatca tgaaaacgac cacgatttac taaaaaaagc caatacttta
     4321 ggcatgctga atgcacaaga agcacaaaca ggttatgtca atttaaataa ttatgatgat
     4381 ttattcaatc aaattgaagt tttagaggtg taaaaaatgt caaaatcgaa tcaaaaaatc
     4441 gcatcaattg aacaattaag taacaacgaa ggtatcattt ctgcattagc atttgaccaa
     4501 cgtggtgctt taaaacgcat gatggcaaaa catcaaactg aagaaccaac tgtagctcaa
     4561 attgaacaat taaaagtatt agttgcggaa gaattaacac aatatgcatc ttcaatttta
     4621 ttagatcctg aatatggttt accagcttca gatgcacgta acaaagattg tggattatta
     4681 cttgcttatg aaaaaactgg atatgacgta aacgctaaag gtcgtttacc tgactgttta
     4741 gtagagtggt ctgcaaaacg tttaaaagaa caaggcgcaa atgctgttaa attcttactt
     4801 tactatgatg ttgatgatgc tgaagaaatt aacattcaaa agaaagcata cattgaaaga
     4861 atcggttcag aatgtgttgc agaagatatt ccattctttt tagaagtatt aacatacgat
     4921 gacaacattc ctgataacgg tagtgttgaa ttcgcaaaag ttaagcctag aaaggttaac
     4981 gaagcaatga aattattctc agaaccacgc tttaacgttg atgtattgaa agttgaagta
     5041 ccagtaaaca tgaaatatgt agaaggcttt gctgaaggtg aagtggttta tacaaaagag
     5101 gaagcggcac aacactttaa agatcaagat gcagcaactc atttaccata catttactta
     5161 agtgctggtg tttctgcgga attattccaa gaaactttaa aatttgctca cgaagctggt
     5221 gctaaattca acggggtact ttgtggccgt gctacatggt ctggtgctgt tcaagtatac
     5281 attgaacaag gtgaagatgc tgctcgtgaa tggttacgta caactggttt caaaaatatt
     5341 gatgacttaa ataaagtatt aaaagatact gcgacatctt ggaaacaaag aaaataagtt
     5401 taaaagggga ttaatgctat gaatagagaa gaagtccaat tattaggttt tgaaattgtt
     5461 gcatttgcag gggatgcacg ttctaagttt ttagaagcat taacagcagc tcaagctgga
     5521 gattttgcaa aagcagatgc actgattgaa gaaggaaaca attgcattgc ggaagcgcat
     5581 agagcacaaa caagcctgtt ggctaaagaa gcgcaaggtg atgatattgc atacagtgta
     5641 acgatgatgc atggtcaaga tcatttaatg acaacaattt tactgaaaga tttaatgaag
     5701 catttattag aattttataa aagagggtga tgacaatgat gcaaaaattg atcgcacaaa
     5761 ttgaaaaagg gaagccattt ttcgagaaat tatcacgtaa catatattta agagccatcc
     5821 gtgatggatt tatttctgca atgcctgtca tcttattctc aagtatcttt ctattaattg
     5881 catatgtgcc aaatatattt ggttttaaat gggataaagg catggaagcc attttaatga
     5941 aaccatacaa ctatacgatg gggcttgtag catttttagt agcaggaacg acagcgaaat
     6001 ctttaacaga ttcatttaac cgtaagctag aaagtacaaa tcaaattaac tttatttcta
     6061 caatgctagc agcgatgtgt ggattcttgt tcttagcttc tgatccagcc aaagatggtg
     6121 gattcttaag tgcatttatg ggaactaaag gtttattaac agcattctta tcagcatttg
     6181 taactgttat tgtttataac ttctgtgtga agcgcaatat tacaattaaa atgccgaaag
     6241 aagtaccgcc gaatatttca caagtattta aggacttaat tccattttca gcggtaatca
     6301 tcattcttta tgcattagat ttagtcattc gcaacagctt taaatcaaat gtagcggaag
     6361 gtattttaaa attattcgaa ccattattta cagcagcaga tggatggatt ggtgtcacaa
     6421 ttatctttgg tgcctttgca ttattctggt ttgtaggtat tcatggtccg tcaattgtag
     6481 agccagcaat tgcagccatt acatatgcga atatcgaagc gaacttcaag ttgcttcaag
     6541 ctggagaaca cgcagataaa attattacat caggtacaca aatgtttatc gttacatttg
     6601 gaggtacggg tgcaacattg gtcgtcccgt tcatgtttat gtggatgacg aaatctaaac
     6661 gaaacaaagc gattggacgt gcatcagttg taccaacatt ctttggtgta aacgaaccaa
     6721 ttttatttgg tgcaccacta gtattgaatc ctgtgttctt tattccattt gtattagcac
     6781 caattgttaa cgtatggatt ttcaaacttt tcgttgaagt gttaggaatg aatagtttta
     6841 gtgtgaattt accttggaca acaccaggtc cattgggcat tatcatgggt acaggttttg
     6901 gtttatggtc attcgtacta gctattactt tgattgttgt agatattatt atttactacc
     6961 cattcctaaa agtttatgat agtgaaattc ttgatgaaga agaaggacgt aaagaaagta
     7021 attcagattt aaaagaaaaa gttgcagcaa actttgatac gaaaaaagct gattcaattt
     7081 tagcggcaag tggtgtatca gacgatgcag ctaaagcttc aaatatcaca gaacaaacaa
     7141 atgtcttagt actttgtgcc ggtggtggta caagtggatt attagccaat gcacttaata
     7201 aagcagctga agaatatcat gtgccagtga aagcggcagc tggcggatat ggcgcacata
     7261 tggacattat gaaagagtac caattaatca ttttagcgcc acaagttgcg tctaactatg
     7321 aagatattaa gcaagataca gatagattag gtattaaatt agcgaagact caaggtgctg
     7381 aatatatcaa attgacacga gatggccaag cagcccttga tttcgttcaa caacaatttg
     7441 aaaattaaga ataggagttt ttcatatgac taaaacatta cctgaagatt ttatttttgg
     7501 tggagcaaca gcagcatatc aagcagaagg tgcgacaaat acggatggta aaggtcgcgt
     7561 agcatgggat acgtatttag aagaaaacta ttggtacact gcagaaccag cgagtgattt
     7621 ttataacaga tatccagtgg atttagaatt aagtgaaaaa ttcggtgtta atggtatccg
     7681 aatttcaatt gcatggtctc gtatcttccc aaatggatat ggcgaagtta accctaaagg
     7741 tgttgagtat taccataagt tatttgcaga gtgtcataaa cgtcatgttg aaccatttgt
     7801 aacattgcat cattttgata caccagaagt gttacacaaa gatggcgatt ttctaaatcg
     7861 taaaacgatt gactactttg tagattatgc tgaatattgt tttaaagaat tcccagaagt
     7921 aaaatattgg actacattca atgaaattgg acctattggt gatggtcaat atttagtagg
     7981 taaattccct ccaggtatta aatatgattt tgaaaaagta ttccaatcac atcataacat
     8041 gatggtggct catgctagag cagtaaaatt atttaaagat ggcggataca aaggagaaat
     8101 tggtgttgta cacgcattgc caacaaaata tccgttcgat ccatcaaatc ctgaagatgt
     8161 tcgtgcagca gaattagaag atatcatcca taataaattc atcttagatg caacatattt
     8221 aggtaagtat tctcgtgaaa cgatggaagg tgtgcaacat atcttatctg ttaatggcgg
     8281 taaattaaac attacagatg aagattatgc cattttagat gcagccaaag atttaaacga
     8341 cttcttaggt atcaattact acatgagtga ttggatgaga ggttacgatg gagaatctga
     8401 aatcacgcat aatgcgacag gtgataaagg cggttctaaa taccaactta aaggtgtcgg
     8461 acaacgtgaa tttgatgtcg acgtacctcg cacagactgg gactggatga tctatcctca
     8521 aggtttatat gatcaaatca tgcgtgttgt taaagattat cctaactatc ataagattta
     8581 tatcactgaa aatggtttag gatataaaga tgaatttatt gaatctgaaa aaacagttca
     8641 tgatgatgca cgtattgatt atgtaagaca acatttgaac gttattgcag atgctattat
     8701 agatggtgca aatgttaaag gttactttat ttggtcatta atggatgtgt tctcttggtc
     8761 aaatggttat gaaaaacgat atggtttatt ctatgtcgat tttgagactc aagaacgtta
     8821 tcctaagaaa agtgcttatt ggtacaaaga gttagccgaa acaaaagaaa ttaaataatt
     8881 atagatatta aaaggcctat cagtcgagtg ctggtaggtc tttatttata gaaagagata
     8941 aattaaacat gcatatacat atttatatat taatcaatgg gtgacacacc attgaaagct
     9001 aaagaagcaa tcaattaaca aacgttaacg gacaactttc caaagtcatc ttaaaagtta
     9061 atgatgtatt caaaatcaac taaaggacat ttttaatcac atcctttata cttatataaa
     9121 agacatgaat gggagttaaa agtgtgaaga aaatttttgt aattattacg acattgttag
     9181 cggtcgctat tattataggc tctattatta tggtggtgtt tagtcaacga caggcacaaa
     9241 catttaaaat acaacaacag caatttgtta aaaagccaat tccaacattg ttcttacatg
     9301 gatttggtgg tagtgccaat tctgaaaagt ttatggtgaa gcaagcagaa aaaagagggg
     9361 taactaaaga tatcatcact gcttacgttt caaaggatgg tgcagtgaca tttaaaggga
     9421 aactaagaaa agatgcagtg aatcctattg tgaaaattga acttgaaaat aacagacaag
     9481 gctatttgga taagaatgct gcgtggttta aaaatgtact gactaagtta caatctgaat
     9541 acaatttcga taaattcaac tttgtcggtc actcaatggg gaacttaaca tttgcacaat
     9601 atatgatgac atatggtaac gacaagtcac tgccacagtt aaacaaacaa gtgaatattg
     9661 cagggacatt taatggtgtt ttaaatatga atgaagatgt caatgaaatt acagttgata
     9721 aagatggtaa accaagtcga atgaatcaac cttatcagca attacgcgtg ctaaaagata
     9781 tttataaagg taaaggtatc gaagtgctaa atatttatgg ggatttgaaa gatggtacac
     9841 attcagacgg cagagtgtct aatagttctt cgaaatcatt aaaatattta ctgggaaata
     9901 gtccgaaaag ttatcgagaa tctaaatatg aaggtgagcc tgcacaacat agtcagcttc
     9961 atgagaatga aaatgttgct aatgagttaa tcgacttctt atggaaaaaa taaagcaact
    10021 gctagttttt ctggggcaaa aaagcttgta atattagagc ttttcaaaaa ttatctattg
    10081 tttgagagtt tccataaaat gtctaagtcg taacatttta aatttatgat tatagcaaat
    10141 ataatgtggt tgaagttaca tgaatgaatc agtgcttaag tcaaaatata acaactaaga
    10201 atgtatttgc atacagacat cacgaagaca caattcaaac ttaacgctta agtaacaaat
    10261 aagtaaaagt ttcaaataaa gaaaggtgat taatgatgca aaataaacaa attcttttta
    10321 acaaaatacc agaaggcatg cctcaagagg atacattcaa aattgaagag attgacacgc
    10381 ctaaacttga gagcgatggt gtacttgtac aaacgctgta tatttcagtg gatccatata
    10441 tgagaggacg tatgactaag gcagattcgt atgtacagcc ttttgaaatt ggtaaaccga
    10501 ttgttagcca tgtcgttgca aaagtaattg attcgacact cgcagattat aaaaaaggag
    10561 atgtcgttgt aggtatgttg ccttggcgaa tcattaatca tgtccaagcg gatcaaatta
    10621 ctaaagttcc aacgacagat gtaccacttg atttatattt aagtgtttta ggaatgccag
    10681 gacaaacagc atatcatggt ctgcttgata ttggacaacc taaagccggt gatactgttg
    10741 tggtttcagc tgcatctggt gctgtaggtt cagttgtagg tcaaattgct aagattaaag
    10801 gttgccgtgt agtaggtatt gcgggtggcg acaaaaaagt aaattattta acagagacac
    10861 tgggctttga cgctggtatt gattataaga aagaggattt tgcagaagcg ttagcacaag
    10921 cagtaccaga tggtattgat gtttattttg aaaatgttgg tggaacagtt ggcgatgaag
    10981 tatttaagca tttaaatcgt tttgcacgtg taccagtatg tggcgcaatt tcgtcatata
    11041 atcatcctga agccgatatc ggaccacgta ttcaaggtac gcttattaag aagcaagtaa
    11101 tgatgagagg cttcttggta agtgaattcg ctaatgcttt taaagaagct agtgaacagc
    11161 ttgcaacatg ggttcaagaa ggtaaaattc agtcacaagt aaccattgaa gatggttttg
    11221 aaaatgcgcc acatgcattt aaaaatttat ttactggcga taactttggt aaacaagtta
    11281 ttaaagtaac ggaataataa agtatacgtt ttaacaaact aactttgtta attgaccaaa
    11341 atttaataat tttttatcta aagtaaattt gttcagcaac atggtagaaa taagtctaaa
    11401 atttaatacg aaagtcgtct gtaattattt atatattcaa atgaaaaagg agtcatgatg
    11461 ctgcgatata agcacacatg gcttctattt tgtataataa gaaataagtt cataaatgag
    11521 gtgaaacgat atgaaaatga taggatttga gaagcccttt aaattggaag aaggaaattt
    11581 atttaaagta tacgaacaaa gaaagccaac gccagaaaac gatgatatct tagtcaaagt
    11641 aaatagcatt agtgtaaatc ctgtagatac aaagcaacga caaatggaag tcactcaagc
    11701 acctcgtgta cttgggtttg atgctattgg tactgttgaa gctatcggac cagacgttac
    11761 attatttagt ccgggtgacg ttgtatttta tgctggatcg ccaaatagac aaggatcaaa
    11821 tgcgacatat caattggtgt cagaagctat agttgcaaaa gcaccacaca acataagtgc
    11881 taatgaagcg gttagtctac cgctaactgg tattacggca tatgaaacgt tttttgatac
    11941 atttaaaatt tctacgaatc ctgctgaaaa cgaagggaag tctgtcttaa tcattaatgg
    12001 tgccggtggt gtaggcagta tagccactca aattgctaaa cggtatggtt taactgtcat
    12061 tacaacagca tcacgacaag aaacaacaga gtggtgtgaa aagatgggcg cagatatcgt
    12121 attgaatcat aaagaggatt tagtgcgtca atttaaagaa attccattag tagattatat
    12181 attttgcacc tataatacag acttgtatta caatactatg attgaactga taaaaccatt
    12241 agggcatatt acgactatcg tagcttttaa tgaagatcaa gatttaaatg cattaaaatt
    12301 aaaaagtata acatttacac atgaatttat gtttgcgcgt ccaattcatc gaacgcctga
    12361 tatgattaaa caacatgagt atttagaaga tattacgaaa aatatagaat cagggcatta
    12421 ccaaccaaca acgacacaag tttttgaagg tttatcacct gaaaacttat atcaagcaca
    12481 tcaattgttg gaaaaacaat caatgattgg aaaattagta attaatatat agattagtaa
    12541 gtaaagtcgc cttatgcaga aaatactgta taggcgactt attttttgtg tctagcgact
    12601 attgattcta aacggaaata tggcaacttt gcaggtatca aatcaataat gcactagaaa
    12661 ggcattactt aaatacaata aaatactttg ttgctcaatg ataaataatt tgagtgtgtt
    12721 ttatatatca tttttaaaag atgaggcatt attaattgtg tctatataaa attacaaatg
    12781 attaaaatgt tgcagttatg ggtatgaact taccattaca cagaaagggt ttgtatttat
    12841 gaaaaataaa tcagtaagtc tagtgttttg ggtatcttta gtgatatgta cgatatttgt
    12901 tgcttttgga gcaatatttc cgaaacagtt agaaaaactc acgcaaaaca ttactacatt
    12961 tattgcgcta catttttctt ggtattattt attgctcgtt ttagttattt tatttgtctg
    13021 tgtgtatatt ttattttcga gatacgcaaa cattacatta ggtgaagaag gcgaagatcc
    13081 tgaattctca ttgccgtctt ggtttgctat gttatttagt gctggtatgg gaataggact
    13141 tgtgttctgg acaacggcag aaccaatcag tcatgccttc aaattaacgc ctattcataa
    13201 agctggcacg caatctgcaa ttaatgatgc aatgcagttt tcatttttcc attgggggat
    13261 tcatgcatgg gcagtctatg gtattgtcgc gctagtgttt gcatatttta gctttcataa
    13321 aggatatcct ggcttagtca gcgctacatt gacgccttta ttaggtgaaa aggcaatgcg
    13381 tggaccttta ggtggtgcca ttgatgtatt ggcagtcata gctacagtaa caggcgttgc
    13441 tgcaacatta ggtttcggtg cattgcaaat aaacgaaggt ttacattttt tatttaatgt
    13501 accgagtaac ttcacgatgc aagtaatctt gattgtcatt gcaaccatat tatttacttg
    13561 gtcagcatgg tcaggtatcg ataaaggtat caaaacgttg agtaatatca acatgttact
    13621 tgcttttgta gtacttattg gtctttttat tgttgggcca acgctataca ttttaaatac
    13681 atttacgaat ggtctaggta attatattgc aaatttcttt agtatgagtt tacgcattcc
    13741 gacaggtggt caaaaattcc aatggttaca aaattggaca atcttttact gggcatggtg
    13801 gatatcttgg gcaccatttg taggtatatt tattgcacgt gtgtcaaaag gacgtacgat
    13861 taaagaattt attctaggtg tactttttgt cccggcgtta gtttgcttta tattctttgc
    13921 ggtattcggc gcgtcagcaa tttacttaca agataatcat attgccgata ttgctaaagc
    13981 tgcgacagaa acagcaactt ttgcgacgct acaacattat ccgctaggtt ttgtattaag
    14041 cttaattact ttacttgtta ttatgatttt ctttgttaca tcagcggatt cggcaactta
    14101 tgttttaggt atgttgagtt caagtggtga tattaatccg aagtcatttg tcaaagtgag
    14161 ttggggtatc attatggcat tatttgcaat catcatgatt tacacaggtg gtactcaagc
    14221 aattcaaaat ttattaatca ttgctgcgtt accattttcc gtggtcatta tagcgatgat
    14281 atggtcgctt ataaaatctt tgagtgagga gaaaccgaga aatagtaata aggttttaat
    14341 taaacatcgt gacccagata ttttagaata tcgttcttca aaacatattg acgaaaatta
    14401 attaataatg aggttttatt tacaaaaact agggaatat
//
LOCUS       DALAGD010000077        14232 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.078, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000077 DALAGD010000000
VERSION     DALAGD010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14232
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.078"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(<1..177)
                     /locus_tag="O2701_001990"
     CDS             complement(<1..177)
                     /locus_tag="O2701_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCZ4293873.1"
                     /translation="MDLFTRKDGTSIHYSTLGEGYPIVLIHTVLDNYSVFNKLAAELA
                     KSFQVVLIDLRGHGY"
     gene            complement(161..880)
                     /locus_tag="O2701_001991"
     CDS             complement(161..880)
                     /locus_tag="O2701_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCZ4293874.1"
                     /translation="MDLNQIKAVVFDLEGTLLDRVKSREKFIEEQYERFHDYLIHVQL
                     ADFKKAFIELDDDEDNDKPDLYKEIIKRFHVDRLTWKDLFNDFEMHFYRYVFPYYDTL
                     YTLEKLSQKGFQIGVIANGKSKIKQFRLHSLGLMHVINYLSTSETVGFRKPHPKIFED
                     MIDQLGVLPEQIMYVGDDALNDVAPARAMGMVSVWYKQEDAEIEPLEEEVDFTITTVE
                     ELLTILPIKNDNKGENYGSIY"
     gene            complement(935..1885)
                     /locus_tag="O2701_001992"
     CDS             complement(935..1885)
                     /locus_tag="O2701_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCZ4293875.1"
                     /translation="MTFNKVLLSWIVILIITTSIYLFWQLGDINDVFNQSILINVRLP
                     RLLEALLTGMILTVAGLIFQTVLNNALADSFTLGLASGATFGSGLALFLGLTTLWIPV
                     FSITFSLITLITVLVITSVLSQGYPVRILILSGLMIGALFNSLLYFLILLKPRKLNTI
                     ANYLFGGFGDAEYSNVSIIAITFIIALFGIFIILNQLKLLQLGELKSQSLGLNVQLIT
                     YIALCIASMITAINVAYVGIIGFIGMVIPQLIRKWQWKQSLGRQLALNIVTGGQIMVM
                     ADFIGSHILSPVQIPASIIIALIGIPVLFYMLISQSKRLH"
     gene            complement(2033..2920)
                     /locus_tag="O2701_001993"
     CDS             complement(2033..2920)
                     /locus_tag="O2701_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCZ4293876.1"
                     /translation="MKKSLIAFILIFMLVLSGCGMKDNDKQGSNDNGSSKSPYHRIVS
                     LMPSNTEILYELGLGKYIVGVSTVDDYPKDVKKGKKQFDALNLNKEELLKAKPDLILA
                     HESQKATANKVLSSLEKQGIKVVYVKDAQSIDETYNTFKQIGKLTHHDKQAEQLVEET
                     KDNIDKVIDSIPAHHKKSKVFIEVSSKPEIYTAGKHTFFNDMLEKLEAQNVYSDINGW
                     NPVTKESIIKKNPDILISTEAKTRSDYMDIIKKRGGFNKINAVKNTRIEVVNGDEVSR
                     PGPRIDEGLKELRDAIYRK"
     gene            complement(3299..3934)
                     /locus_tag="O2701_001994"
     CDS             complement(3299..3934)
                     /locus_tag="O2701_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HCZ4293877.1"
                     /translation="MLGTDELYKVLYEHLGPQFWWPADNDIEMMLGAILVQNTRWRNA
                     EIALNQIKEHTHFNPNHILELPIETLQSLIHSSGFYKSKSLTIKTLLTWLARHHFNYQ
                     EINERYKGGLRKELLSLKGIGSETADVLLVYIFGRIEFIPDSYTRKIYDKLGYENTKN
                     YDQLKKVVTLPNHFTNQDANEFHALLDVFGKHYFRDKDIKNYDFLEPYFKK"
     gene            complement(4322..5983)
                     /locus_tag="O2701_001995"
     CDS             complement(4322..5983)
                     /locus_tag="O2701_001995"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCZ4293878.1"
                     /translation="MNIIDQVKQTLVEEIAASINKAGLADEIPDIKIEVPKDTKNGDY
                     ATNIAMVLTKIAKRNPREIAQAIVDNLDTEKAHVKQIDIAGPGFINFYLDNQYLTAII
                     PEAIEKGDQFGHVNESKGQNVLLEYVSANPTGDLHIGHARNAAVGDALANILTAAGYN
                     VTREYYINDAGNQITNLARSIETRFFEALGDNSYSMPEDGYNGKDIIEIGKDLAEKHP
                     EIKDYSEEARLKEFRKLGVEYEMAKLKNDLAEFNTHFDNWFSETSLYEKGEILEVLAK
                     MKELGYTYEADGATWLRTTDFKDDKDRVLIKNDGTYTYFLPDIAYHFDKVKRGNDILI
                     DLFGADHHGYINRLKASLETFGVDSNRLEIQIMQMVRLMENGKEVKMSKRTGNAITLR
                     EIMDEVGVDAARYFLTMRSPDSHFDFDMELAKEQSQDNPVYYAQYAHARICSILKQAK
                     EQGIEVTAANDFTTITNEKAIELLKKVADFEPTIESAAEHRSAHRITNYIQDLASHFH
                     KFYNAEKVLTDDIEKTKAHVAMIEAVRITLKNALAMVGVSAPESM"
     gene            complement(5980..6396)
                     /locus_tag="O2701_001996"
     CDS             complement(5980..6396)
                     /locus_tag="O2701_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HCZ4293879.1"
                     /translation="MDKKVSIQTKQVLKQHNEKEKFEFTTEGTWQQRQSNFIRYVEQI
                     EDATVNVTIKVDDDSVKLIRKGDINMNLHFVEGQTTTTFYDISAGRIPLEVKTLRILH
                     FVSGDGGKLKIHYELYQDNEKMGSYQYEINYKEIGE"
     gene            complement(6489..6596)
                     /locus_tag="O2701_001997"
     CDS             complement(6489..6596)
                     /locus_tag="O2701_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293880.1"
                     /translation="MKDSENLVFFGFEVLFVFYKMAYIYEALIKYGIVN"
     gene            complement(6671..7681)
                     /gene="adhP"
                     /locus_tag="O2701_001998"
     CDS             complement(6671..7681)
                     /gene="adhP"
                     /locus_tag="O2701_001998"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCZ4293881.1"
                     /translation="MRAAVVTKDHKVSIEDKKLRALKPGEALVQTEYCGVCHTDLHVK
                     NADFGDVTGVTLGHEGIGKVIEVAEDVESLKIGDRVSIAWMFESCGRCEYCTTGRETL
                     CRSVKNAGYTVDGAMAEQVIVTADYAVKVPEKLDPAAASSITCAGVTTYKAVKVSNVK
                     PGQWLGVFGIGGLGNLALQYAKNVMGAKIVAFDINDDKLAFAKELGADAIINSKDVDP
                     VAEVMKLTDNKGLDATVVTSVAKTPFNQAVDVVKAGARVVAVGLPVDKMNLDIPRLVL
                     DGIEVVGSLVGTRQDLREAFEFAAENKVTPKVQLRKLEEINDIFEEMENGTITGRMVI
                     KF"
     gene            complement(8180..8701)
                     /locus_tag="O2701_001999"
     CDS             complement(8180..8701)
                     /locus_tag="O2701_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwhD family protein"
                     /protein_id="HCZ4293882.1"
                     /translation="MEVISLSEKKGFNFNIIKNDPLDGHKGTNIGSISLDNIAPVFID
                     VANKEAFIDIGGMHARAKVEKGVKWITDKAAVEGDEAKEYWLCWVTTERNEQGPYYAG
                     LTACYLLVNKAIRRGYKSMPEHVNMMDKSMKHHIIIDQIGDENKAILKDFLMNHDEGM
                     WKHSSDALHQAFN"
     gene            complement(8729..10024)
                     /locus_tag="O2701_002000"
     CDS             complement(8729..10024)
                     /locus_tag="O2701_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCZ4293883.1"
                     /translation="MTNAYVDLKLVEEKVFKDPIHRYIHVEDQLIWDLIKTKEFQRLR
                     RIRQLGTLYLSFHTAEHSRFGHSLGVYEIVRRLIDESFIGHDAWDNKDRPLALCAALL
                     HDLGHGPFSHSFEKIFNTDHEAYTQAIITGDTEVNAVLRKVAPEFPREVAEVINKTHH
                     NKLVISMISSQIDADRMDYLQRDAYFTGVSYGAFDMERILRLMRPSKDEVLIKESGMH
                     AVENFIMSRYQMYWQIYFHPVSRGGEVLLNNCLKRAKQLYNEGYEFKLHPHDFIPFFE
                     ETVTIEQYVELDEAVVTYYLEKWTKEDDAILSDLASRFINRDLFKYIPFDGSIITISE
                     LQELFEAGGINPDYYFVSEAFSDLPYDYDRPGSNRKPIHLLRQDGTIREISNQSLVIH
                     SITGINRQDYKLYYPREMVAKIKDKTIREAIENLINELN"
     gene            complement(10064..10540)
                     /locus_tag="O2701_002001"
     CDS             complement(10064..10540)
                     /locus_tag="O2701_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4293884.1"
                     /translation="MFKVRQATEKDVVQIRDVATKAWFNTYLNIYAATTVNHLLEASY
                     NEHHLKKRLQEQLFLVVEEGNDIVGFANFIYGEELYLSAHYVKPESQHTGYGTALLNE
                     GLSRFEDKFEGVYLEVDNKNEEAVAYYKEQGFTILRSYEPEMYGEKLDLALMYKAF"
     gene            complement(10709..11245)
                     /locus_tag="O2701_002002"
     CDS             complement(10709..11245)
                     /locus_tag="O2701_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HCZ4293885.1"
                     /translation="MITVLFGGSRPNGNTAQLTKFALQDLEYQWIDVTQHQFKPIRDV
                     RHTAETITSYDDDYLPILDKILASDTIIFASPVYWYSISAPLKAFIEHWSETLQDKRY
                     PNFKAQMAEKDFRVILVGGDCPKIKAKPAITQMKYSLDFLGATLNGYIIGTAEKPGDI
                     MKDNYALARATEWNSILQ"
     gene            complement(11456..12394)
                     /locus_tag="O2701_002003"
     CDS             complement(11456..12394)
                     /locus_tag="O2701_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCZ4293886.1"
                     /translation="MKQFVNLGKSDVEVFPIALGTNAVGGHNLYPNLDEEQGKDVVRQ
                     AINHGINLLDTAYIYGPERSEELVGEVVKEYPREQIKIATKGSHEFDENQEVHQNNQP
                     EYLKQQVENSLKRLQTDYIDLYYIHFPDNNTPKDQAVAALQELKEQGKIKAIGVSNFT
                     LDQLKEANKDGYVDVVQLEYNLLHRENEAVLQYCVDHQITFIPYFPLASGILAGKYDE
                     NTKFSDHRTTRRDFKPGVFEENVRRVKALESIAAAHQTSIANIVLAFYLTRPAIDVII
                     PGAKRAEQVVENIKAADIVLSDDEIQYIDELFPIED"
     gene            complement(12664..13290)
                     /locus_tag="O2701_002004"
     CDS             complement(12664..13290)
                     /locus_tag="O2701_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HCZ4293887.1"
                     /translation="MLLCESKIINKNPKYRIIKYNDEYLMVDIISTWISLFFPFINWF
                     IPKEYVKISREEFENLNIVKPAKKNVFWPVAGSSALLGVALRKYTHLLDIQLDKKLVI
                     AICCITFIGILIFYVRLIKKSSLNIYNTKNKRSKIFLIPTLKNVCFTLFGYILFGGLT
                     MLFLDALLSMSYQNIIVYFVWIAVIMGFFLVNIALIIDKNIHVILKNQ"
     gene            complement(13314..13559)
                     /locus_tag="O2701_002005"
     CDS             complement(13314..13559)
                     /locus_tag="O2701_002005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293888.1"
                     /translation="MYYLKLTRWKNDGLAGFTAGSISAILVYWTNQKNEFGIKDKNDW
                     IGHKLDVGIDAVEKSAEKTVDGVENVMVKLQKVFLII"
     gene            complement(13525..14160)
                     /locus_tag="O2701_002006"
     CDS             complement(13525..14160)
                     /locus_tag="O2701_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HCZ4293889.1"
                     /translation="MLCESKVINKNPKYRVIKYGDEYLMIDLVSTWLTLFLPMINWLI
                     PKKYVKISKKEFDDLNIVKPVKNKAFWPVAGSTILFGVTFRKYIPSLNIQLEKNMVIV
                     ICCAIFLGVLILFLFLNRKLRLEIYNNNSSKGKIILFPSLKNFCFTIFYYFLFGGLSI
                     MALSMLLTLNPQNIIGFIGWLVMTAGFFLLNMSSIIDKKIYVLSKTNTVEK"
BASE COUNT         4567 a         2683 c         1831 g         5151 t
ORIGIN      
        1 atagccatgt ccacgtaaat caattaacac aacttgaaat gattttgcta attctgctgc
       61 taatttatta aacacagaat aattatcaag tacagtatga atcaatacga taggatagcc
      121 ttcacctaat gtactgtaat gtatcgatgt tccatctttt ctagtaaata gatccataat
      181 tttctccttt attatcattt tttattggta aaatggttaa taattcttcc actgttgtaa
      241 ttgtaaaatc aacttcttct tcgagtggtt caatttcagc atcttcttgt ttataccata
      301 cactaaccat gcccatagct cgtgctggag ctacatcatt taacgcatca tcgccaacat
      361 acataatttg ctcaggtaat acccctagtt gatcaatcat atcttcaaaa attttaggat
      421 gtggtttacg aaaaccaact gtttctgatg ttgataaata attaataaca tgcatcaaac
      481 caagtgaatg taatcgaaat tgtttaatct tagatttacc atttgcgata acaccaattt
      541 gaaagccttt ttgcgatagc ttttctagtg tatacaaagt atcgtaataa ggaaatacat
      601 aacgataaaa atgcatttca aaatcattaa ataagtcttt ccaagttaac ctatctacat
      661 ggaaacgttt aatgatttct ttatataaat caggtttatc attatcttca tcgtcatcta
      721 gctcaataaa tgctttttta aaatctgcca gttgaacatg aattaagtag tcatgaaatc
      781 gttcatattg ctcttcgata aatttctctc gagatttaac tctgtccaac aacgtacctt
      841 ctaaatcaaa tacaactgct ttaatttgat tcaaatccat cgcttatgcc ctctttatgt
      901 ttatagttat ttttatttta gcaaatgtcg tgtgctagtg taaccgtttc gactgagata
      961 ttagcatgta aaataacact ggtataccaa ttaatgcaat gataatactt gccggtattt
     1021 gtactggtga caatatatgg ctaccaataa aatctgccat aaccattatt tgtcctccag
     1081 ttacaatatt taaagccaat tgtcttccta atgattgttt ccactgccat tttctaatga
     1141 gttgcggtat caccatacca atgaatccaa tgatgccaac atatgcgaca tttatcgccg
     1201 ttatcataga agctatacat aacgcgatat atgtaatcaa ttgaacattt aagcctagtg
     1261 actgactttt tagttctcct aattgcaata actttagttg attaagaatg ataaatatac
     1321 caaacaatgc aatgataaat gtgattgcta ttatagatac atttgagtat tctgcatcac
     1381 caaaaccacc aaacagataa ttggcaattg tatttaattt gcgaggtttt aataaaatca
     1441 aaaaatatag aagtgaattg aataacgcac caatcattaa accacttaat attaagattc
     1501 taactggata gccttggctc aataccgacg taatgactaa tacagttatt aatgttatca
     1561 aactaaatgt tattgaaaat acaggaatcc ataacgttgt taaacctaaa aataatgcta
     1621 atcctgaacc aaatgtagcg ccgcttgcca atcctaatgt aaagctatct gccaatgcat
     1681 tatttaaaac tgtttgaaat ataaggcctg caacagttaa tatcatacct gtcaacaatg
     1741 cttctaataa tctcggtaat ctaacattga ttaaaataga ctggttaaat acatcattga
     1801 tatcgcccaa ctgccaaaat agatatatgc tagttgttat aatcaatatg actatccagc
     1861 tcaataatac tttattaaat gtcatagcat tctcttttct ttcatcgata attacattca
     1921 ttatagcaat cacatactcg cgttgctatc attcactaaa tatattttaa aaacccctta
     1981 tctcaaacat gtatttttta catgtagcaa taaggggcat aattagaatg gtttattttc
     2041 tataaattgc atctcttaat tcttttaatc cttcatcaat acgtggacct ggtcttgata
     2101 cttcatcacc atttacaact tcaatacgtg tattcttgac agcattaatt ttattgaatc
     2161 cacctctttt tttgatgata tccatataat ctgatcttgt cttagcttcc gtcgaaatta
     2221 atatatctgg gttcttttta ataatacttt ccttcgttac agggttccaa ccattaatgt
     2281 cactatacac attttgggct tctaattttt ctaacatatc attaaaaaat gtatgcttcc
     2341 ctgctgtata tatttcaggc tttgatgaaa cctcaataaa tacttttgat tttttatgat
     2401 gagcaggaat tgaatctatg actttatcga tattatcttt agtttcctca acaagttgtt
     2461 cagcctgctt atcatgatgc gttaatttcc caatttgctt aaatgtgttg taagtttcat
     2521 caattgattg tgcatcttta acatacacta ctttgatgcc ttgtttctct aatgatgaca
     2581 atactttatt agcagttgcc ttttgcgact catgcgcaag aattagatct ggctttgcct
     2641 ttaaaagttc ctctttattt agattcaaag catcaaattg tttcttaccc tttttcacat
     2701 cttttggata atcatcaacc gttgaaacac caactatgta tttacctaat cctaattcat
     2761 ataaaatttc agtattacta ggcattaacg aaacaattct atggtacggc gatttagacg
     2821 agccattatc attgctacct tgtttatcat tatctttcat accacagcca ctcaggacaa
     2881 gcataaaaat caaaataaaa gcaattaacg atttcttcac actatcacat ctccttttta
     2941 atcccacttt tcgctttagc gattagaggg atttatgcag ttgtgcggta gcatccaact
     3001 gcgttcctct tcatctttag cgattagagg cttttatgca gttgtgcctt agcatccaac
     3061 tgcgttcctc ttcaccttta gtgattagag gcttttatgc agttgctgca caaacaactg
     3121 tattccttta ctccccttga gctttagcga ttaggggctc ttatgcagtt gctgcacaaa
     3181 caaacaactg tttttttctt tatatgcttc tatttattat ataagtccac atactaaaag
     3241 aaaatagtaa attatttttt atatttcata taaactcatc tatctaactt cacagcgttt
     3301 actttttaaa gtaaggttct aaaaaatcat aattctttat atctttgtct ctaaagtaat
     3361 gtttaccaaa tacatctaac agagcatgaa attcattagc atcttgattt gtaaaatgat
     3421 ttggtaatgt gactactttt tttaattgat cataattttt agtgttttca tatcctaatt
     3481 tatcatatat ttttcttgta tagctatctg gaataaattc aatacgtccg aatatataaa
     3541 caagtaagac atctgctgtt tcacttccaa tacctttcaa agataataat tcttttctta
     3601 atccaccttt atatcgctca ttaatctctt gataattgaa atgatgtcgt gctaaccatg
     3661 ttaataatgt tttaatcgtc agtgatttac ttttataaaa gccacttgaa tgtatcaatg
     3721 attgtaacgt ttcaataggt agttctaata tatgatttgg attaaaatgc gtatgttctt
     3781 taatctgatt caatgcaatt tctgcatttc gccatctagt attttgaact aaaattgcac
     3841 ctaacatcat ttcaatgtca ttatcagcag gccaccaaaa ttgtggtccg agatgttcat
     3901 ataaaacttt atataattca tcagttccta acatttacac cctccatttt agagggagtg
     3961 ggacagaaat gatattatcg taaaatttat ttcgtcgtcc caccccaact tgcattgtct
     4021 gtagaaattg ggaatccaat ttctctttgt tggggccccg ccggcaaggt tgactagaat
     4081 tgaaaaaagc ttgttacaag cgaattttcg ttcagtcaac tactgccatt ataactttgt
     4141 agagcataga acattgattt atgtctcagg ctctcttatg cagttgtgca gagcatccaa
     4201 ctgtgttcct cttaacctta ttcacatact aagcattccc caatatgtca catacctaaa
     4261 aaaacactcc aacacaaagt atctttcgag aaactctacg ttggagtgta tataaatgtt
     4321 cttacattga ttcaggtgcg cttacaccga ccattgccaa tgcatttttc aatgtaattc
     4381 tgaccgcttc aatcatagca acatgtgctt ttgttttttc aatatcatct gttaacactt
     4441 tttcagcatt atagaattta tggaaatgag aagctaaatc ttgaatataa ttagtaattc
     4501 tatgtgccga tctatgctca gcagcacttt caattgtagg ttcgaaatca gctacttttt
     4561 tcaacaattc aatcgctttt tcattagtaa tcgttgtaaa atcattcgca gcagtcactt
     4621 caataccttg ctctttcgct tgttttaaaa ttgaacaaat acgcgcatgt gcatattgag
     4681 catagtaaac tggattgtct tgagattgct ctttcgctaa ttccatatca aaatcaaagt
     4741 gactatcagg actacgcata gttaagaaat aacgtgcagc gtcaacgcca acttcgtcca
     4801 taatttctct taatgtaatc gcattaccag tacgtttact catcttcact tctttaccat
     4861 tttccattaa acgaaccatt tgcatgattt gaatttctaa acgattacta tctacaccaa
     4921 acgtttcaag agatgctttc aaacgattaa tataaccatg atgatcagca ccaaataaat
     4981 cgattaaaat gtcattacca cgttttactt tatcgaagtg gtacgcaata tctggtaaga
     5041 aatacgtata tgtaccgtca tttttaatta atactctgtc tttgtcgtct ttaaaatcag
     5101 ttgtacgtaa ccatgtagcg ccatcagctt catacgtata acctaattct ttcatttttg
     5161 ctaaaacttc aagaatttct cctttttcat ataaagatgt ttcactaaac caattatcaa
     5221 aatgcgtatt gaactctgct aaatcatttt tcaatttagc catttcgtat tctacgccta
     5281 attttctaaa ttctttcaaa cgtgcttctt cagaataatc tttaatttca gggtgtttct
     5341 ctgctaaatc tttacctatt tcaataatat cttttccatt atagccatct tctggcattg
     5401 aataactatt gtcacctaaa gcttcaaaga aacgtgtttc aatcgaacgc gctaagttag
     5461 taatttgatt accagcatca ttaatataat attcacgtgt tacattatag ccagctgcag
     5521 ttaaaatatt agctaaagca tcaccaactg ctgcatttct agcatgacca atatgtaaat
     5581 ctcctgtagg gttagctgaa acatactcaa gcaatacatt ttgacctttt gattcattta
     5641 catgtccaaa ttgatcacct ttttcaattg cttcaggaat aattgctgtt aaatactgat
     5701 tatctaagta aaaattaatg aatcctggac cagcaatgtc aatttgtttt acatgtgctt
     5761 tttcagtatc taagttatca acaatcgctt gagcaatttc acgaggatta cgctttgcaa
     5821 tcttagtcag taccatcgca atattagtag cataatctcc attttttgta tctttaggaa
     5881 cttcaatttt aatatcagga atctcatctg ctaatcctgc tttgttaata cttgctgcaa
     5941 tttcttctac taatgtttgt ttcacttgat caataatatt cattcgccta tctccttata
     6001 gttaatttca tattgataag aacccatttt ttcattatct tgatataatt cataatgaat
     6061 ctttagcttg ccaccgtctc cacttacgaa atgtaaaatg cgtaatgttt taacttctag
     6121 tggaattcgt ccagccgata tatcgtaaaa agttgttgtc gtttgtcctt caacaaaatg
     6181 caaattcata ttaatgtcgc ctttacgaat caacttaacg ctatcatcat ccacttttat
     6241 tgtaacatta actgttgcat cctcaatttg ttctacatac cgaataaagt tagattgcct
     6301 ttgttgccaa gttccttcag tagtaaattc aaatttttct ttttcgttgt gctgtttcaa
     6361 cacttgcttt gtttgaatac ttactttttt atccaattta ctactcacct taatcttcgc
     6421 ataatagaaa tattatagca taaaataagg tcatcttgtt atgccttctt aaagctaaat
     6481 aggttcaatt aattaacaat tccatactta atcaacgctt catatatgta agccatttta
     6541 taaaatacaa acaacacctc aaaaccaaaa aacacgagat tttcactatc tttcatgaaa
     6601 acctcgtgtt tgattataat gatagtgttc agagcactac cttctttatc tatatagtca
     6661 gttgatattt ttaaaattta ataaccattc taccagttat agtaccattt tccatttctt
     6721 caaaaatatc attgatttct tctaattttc ttaattgaac tttaggtgtt actttatttt
     6781 cagcagcaaa ttcaaacgct tcacgtaagt cttgtcttgt accaacaagt gaacctacta
     6841 cttcaatacc atcaagcact aatcttggga tatctaagtt cattttatca acaggtaaac
     6901 caacggcaac aactcttgca ccagctttta caacatcaac cgcttggtta aatggcgtct
     6961 tagcaactga agttaccact gttgcatcta atcctttgtt atcagttaat ttcataactt
     7021 ctgcaactgg atcaacatct ttagaattaa taatagcatc agcacctaat tctttcgcga
     7081 atgctaattt atcatcattg atgtcgaagg caacaatttt agcccccata acgtttttag
     7141 catattgtaa agctaggtta cctaaaccac ctataccaaa aacacctaac cattgtccag
     7201 gttttacatt acttactttt acagctttat aagttgtcac acctgcgcat gtaatagaag
     7261 acgctgctgc tggatctaat ttttcaggta ctttcacagc atagtctgca gtaacaataa
     7321 cttgttcagc cattgcacca tctactgtat aaccagcatt tttcacacta cggcaaagtg
     7381 tttcacgacc tgttgtacaa tattcacatc ttccacagct ttcgaacatc caagcgatag
     7441 acacacggtc tccaattttt aatgattcta catcttccgc aacttcgatg actttaccaa
     7501 taccttcatg acctaaagta acgcctgtaa catcaccaaa atcagcattc ttaacatgta
     7561 aatcggtatg acaaacgcca caatattccg tttgtaccaa cgcttcacca ggttttaaag
     7621 ctcttaactt tttgtcctca atacttactt tgtgatcttt cgttacaact gctgctctca
     7681 taataatgtc ctccttatag tcactccagt atgtatgata ttaattggct attactttca
     7741 aaaaagaaaa cgaataatta tagatgtaat ttttatttgt aatagacgcc agattaaaac
     7801 atactactgt gaccttgttt ttagttagat atcaaccgtt aacttttaac gtttccctag
     7861 ttaatatgtt aaaagaaatg acataatcga tatgctaacg tgtaattttt acttcacata
     7921 tatttattta aagataattt ataatactct tatcttcact cttattataa tgtgaattaa
     7981 ttcacaaagt atatagctat gatcaaaaaa atgtgtcagt tattcaaatt ttttattaac
     8041 tacagattaa tttaatttca aaaaaattaa aaaaactccc aatcaatcta agacattatt
     8101 ttcacctcac gatttacgtc gtcattgaga aatgaatcat atctttatag attaattggg
     8161 aaattttagt ttctaatatt taattaaatg cttgatgtaa agcatcagaa gaatgcttcc
     8221 acataccttc atcatggttc attaaaaagt cttttaaaat agctttattc tcgtcaccaa
     8281 tttgatctat gataatatga tgtttcattg atttatccat catattaaca tgttcaggca
     8341 tacttttata accacgacga attgctttat tcactaataa atagcacgct gttaaaccag
     8401 cgtaatatgg tccttgttca ttacgttctg ttgttaccca acacaaccaa tattctttag
     8461 cttcatcgcc ttcaacagca gctttatcag taatccattt cacacctttt tcaactttgg
     8521 cacgagcatg catgcctcca atatcaataa atgcttcttt gttagcaaca tcgataaaaa
     8581 ctggtgcaat attgtctaag ctaattgaac caatatttgt acctttatga ccatctagag
     8641 ggtcattttt tatgatatta aaattaaagc cttttttctc agacaatgat ataacctcca
     8701 tatttaacga taacaatttt agccctgttt aattaagctc attaatcaaa ttttcaatag
     8761 cttctctaat tgtcttatct ttaatctttg caaccatttc tctaggataa tataatttat
     8821 agtcttggcg attaatgcct gtaatactat gaatgactaa tgattgattg cttatttctc
     8881 taatcgtacc atcttgtctt aataaatgaa tcggtttgcg atttgacccc ggtcgatcat
     8941 agtcatatgg caaatcagaa aatgcttcac tcacaaaata ataatctgga ttaataccac
     9001 ctgcttcaaa cagttcttgc agttctgata ttgtaataat tgagccatca aatggaatat
     9061 atttaaataa gtctcgatta ataaatcgac ttgctaaatc acttaaaata gcatcatctt
     9121 cttttgtcca tttttccaaa taatacgtaa ctaccgcttc atcgagttca acatattgtt
     9181 caatcgtaac tgtctcttca aaaaatggaa taaaatcatg tggatgcaac ttaaattcat
     9241 agccttcatt ataaagctgt tttgcgcgtt tcaaacaatt attaagcagc acttctccac
     9301 cacgactaac tgggtggaag taaatttgcc aatacatttg ataacgactc ataataaagt
     9361 tttcaactgc atgcatacca ctttctttga ttagtacttc atctttagaa ggtcgcatta
     9421 atcttaaaat acgctccata tcaaaagcac catatgatac acctgtgaaa tacgcatcac
     9481 gttgtaaata atccattcta tccgcatcga tttgtgacga aatcatcgaa atgaccaatt
     9541 tattatgatg cgttttatta attacttccg caacttctct tggaaactca ggcgccactt
     9601 tacgtaatac agcattcacc tcagtatctc cagtaataat cgcttgtgtg tatgcttcat
     9661 ggtctgtatt aaatattttt tcaaaactat gtgaaaatgg accatgtcct aaatcatgta
     9721 ataatgcagc acataatgcc aacggtctat ctttattgtc ccatgcatca tgaccaataa
     9781 atgactcatc aattaatcgt ctaactattt catacacacc taaagaatgt ccaaagcgac
     9841 tatgttctgc tgtgtgaaaa gataggtaca gtgttcctag ttgtctaatt cgacgtaacc
     9901 tttggaattc cttagtttta attaaatccc atatcaattg atcttcaaca tgaatatatc
     9961 gatgtatcgg gtctttaaaa actttttctt ctactaattt taaatctaca tatgcgttag
    10021 tcattattcc cctccttttc gtttaatata atatttaatt tacttaaaat gctttgtaca
    10081 taagtgctaa gtctaacttt tcgccataca tttctggctc ataagagcgt aagattgtaa
    10141 aaccttgctc tttatagtaa gctactgctt cttcattttt attatctact tctaagtaaa
    10201 caccttcaaa tttatcttca aaacgtgata atccttcatt taacaatgct gtaccataac
    10261 ctgtatgttg cgattctggt ttaacataat gagctgataa atataattct tcaccgtaaa
    10321 taaagttagc aaagccaacg atgtcattac cttcttcaac gactaagaat aattgttctt
    10381 gaagtctttt ctttaaatga tgttcattat atgaagcttc taacaagtga ttaactgttg
    10441 tcgcagcgta tatatttaag tatgtattaa accaagcttt agttgcgaca tctctaattt
    10501 gaacaacatc tttttcagtt gcttgtctta ccttgaacat gactttctcc ccttattaac
    10561 aagttttaat aacggcatta taccacaact tgctcaatac ttaataaaca atgattgtct
    10621 attcaattta tatatttata ttttccgtta aaattaaaaa taaaaaataa cgaagcaaaa
    10681 aatcacttcg tttagtatga ggtatgtctt attgcaatat actattccac tcagttgcac
    10741 gtgctaaggc atagttgtct ttcatgatgt caccaggctt ttcagcagtt ccaataatat
    10801 aaccatttaa agtggcacct aaaaagtcta aactatattt catttgcgta attgctggct
    10861 tcgcttttat ttttggacaa tctccaccaa ctaaaataac tctaaaatcc ttttcggcca
    10921 tttgtgcctt aaaattagga tatcgtttat cttgtaatgt ttctgaccaa tgttcgataa
    10981 atgctttcaa tggtgctgaa atgctatacc aatacactgg tgatgcaaaa ataattgtat
    11041 cactagccaa tattttatct agaatcggca aatagtcatc gtcatatgaa gtaatagtct
    11101 ctgctgtatg tctcacgtca cgtatcggtt taaactgatg ttgtgtcacg tcaatccatt
    11161 gatactctaa atcttgcaaa gcgaattttg ttaattgtgc agtattaccg tttggtctac
    11221 tcccaccaaa caaaacagta atcattttag cctaacctca cttttgatta ataaatatct
    11281 gtgtttttcg ttacctaatt atactatcat aagctttgcc taccgaatag taaaacgctt
    11341 acaactttta tataaatttg acgaaatttc gtcatgcctt atataacgtc gtttgtgata
    11401 cggggctaat tcatgatgaa attagataca tatatcacca ttaaatacaa ttcatttagt
    11461 cttcaatcgg aaacagttca tcgatatatt gaatctcatc atctgataaa acgatatctg
    11521 cagctttaat attttcaacg acttgttctg cacgttttgc accaggaata atcacatcga
    11581 tagctggtct cgttaaataa aatgctaata caatgttcgc aattgaagtt tgatgtgctg
    11641 cagctatgct ttccaaagct tttacgcgac gcacattttc ttcaaataca cctggtttaa
    11701 aatcacgacg tgtagtacga tggtcactaa atttagtgtt ctcatcatat tttccagcta
    11761 aaataccgga tgctaatggg aaatatggaa taaatgtgat ttggtgatca acacaatatt
    11821 gtaatactgc ctcattttcg cgatgcaata aattatattc taactgtaca acatcaacgt
    11881 aaccatcttt atttgcttct ttaagttgat ctaatgtgaa atttgataca ccaattgctt
    11941 taatcttccc ttgttcctta agctcttgta atgctgcaac tgcttgatct ttcggagtgt
    12001 tgttatccgg aaaatgaata taatataaat cgatataatc agtttgtaga cgtttcaaac
    12061 tattctcaac ttgttgtttt aaatattccg gttgattgtt ctgatgtact tcttgatttt
    12121 catcaaattc atgagaccct ttcgtagcaa ttttaatttg ctctcgcgga tattctttaa
    12181 caacttctcc aaccaattct tctgatcgtt ctggcccata aatatatgcc gtatctaata
    12241 aattaatacc atgattaatg gcttgacgaa caacatcttt tccttgttct tcatctaagt
    12301 tcggatataa attatgccca cctactgcgt tcgtcccaag tgcgattgga aacacttcaa
    12361 catcagattt acctaagttt acaaattgct tcattagacc cagctcctta tctttataac
    12421 ttttccacta tcacactatc atgatttaat acaacttcca aatgttagaa ttagagcttg
    12481 aatttaagcg gtgtaatagg aaaacgttat ataaaactca gttaaaataa gtattgctta
    12541 aattgatatg catatagcgc gattgctcgt tgacctttaa attacatgta cttttgaatc
    12601 agaaattgaa aaacatttaa gaacgacaat attaaagcca ttgcaaattt aatttggtaa
    12661 tttctattgg tttttaagta tgacatgaat gtttttatct ataattaaag ctatattaac
    12721 tagaaaaaaa cccattataa ctgcaatcca aacaaaatat actattatgt tttgataact
    12781 cattgataat agtgcatcta ggaatagcat agtcaatccg ccaaataaaa tatatccaaa
    12841 taatgtgaaa caaacatttt ttagtgtagg tattaaaaaa atttttgacc ttttattttt
    12901 agtattataa atatttaaag atgatttttt aattaggcgt acataaaata ttaaaatccc
    12961 tataaatgtg atgcaacata tggcaataac taatttttta tcaagttgaa tgtcaagtaa
    13021 atgtgtatat tttcttaatg caactcccag caaagcagag ctacctgcaa ctggccaaaa
    13081 aacattcttt ttagcaggtt taacaatatt taagttttca aactcttctc tactaatttt
    13141 gacgtattct tttgggatga accaattaat aaaaggaaaa aataaactaa tccaagtgct
    13201 tattatatcg accattaagt attcatcatt atatttaata attctatatt ttgggttttt
    13261 attgatgatt ttagattcgc atagcaacat ttaccagctc catttcttag ggcttatatg
    13321 attagaaata ctttttgaag cttcaccatg acattttcaa caccatctac tgttttttct
    13381 gcagattttt ctacagcatc tataccaacg tctagtttat gtcctatcca atcgttttta
    13441 tcttttattc caaattcatt tttttgattg gtccaatata caagtattgc cgaaatagaa
    13501 cctgcagtaa atccagctaa accatcattt ttccaccgtg ttagttttag ataatacata
    13561 aatttttttg tcaataatcg atgacatgtt taacagaaag aaacctgcag tcattaccaa
    13621 ccaaccaata aagcctatta tattttgagg atttaaagtt aataacatac ttagagccat
    13681 tattgaaaga ccgccaaata aaaaataata aaatattgtg aaacaaaagt tttttaatga
    13741 aggaaataaa attattttcc ctttactaga gttattatta taaatttcca accttagctt
    13801 acgattcaga aataaaaaaa gtattaaaac acccagaaat attgcacaac atattacaat
    13861 caccatgttt ttctctaatt gaatattaag tgaaggaata tattttctaa acgtaactcc
    13921 gaacaaaata gtactacctg caactggcca aaaagcttta tttttaacag gtttgacaat
    13981 gtttaaatcg tcaaattctt ttttactgat tttgacatat ttttttggaa ttaaccaatt
    14041 aatcatagga agaaataaag ttaaccaggt acttactaaa tcaatcatta aatattcatc
    14101 accatattta ataactctat acttaggatt tttgttgata actttagatt cacatagcaa
    14161 tatttatcac taccattcct ttgtttatta ttcctattaa aacttttcaa ttccatttga
    14221 tttaaataat ac
//
LOCUS       DALAGD010000078        13875 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.079, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000078 DALAGD010000000
VERSION     DALAGD010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13875
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.079"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            129..623
                     /gene="tpx"
                     /locus_tag="O2701_002007"
     CDS             129..623
                     /gene="tpx"
                     /locus_tag="O2701_002007"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCZ4293890.1"
                     /translation="MTEITFKGGPIHLKGQQINEGDFAPDFTVLDNDLNQVTLADYAG
                     KKKLISVVPSIDTGVCDQQTRKFNSDASKEEGIVLTISADLPFAQKRWCASAGLDNVI
                     TLSDHRDLSFGENYGVVMEELRLLARAVFVLDADNKVVYKEIVSEGTDFPDFDAALAA
                     YKNI"
     gene            746..1693
                     /locus_tag="O2701_002008"
     CDS             746..1693
                     /locus_tag="O2701_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCZ4293891.1"
                     /translation="MAEQQTIMERLFHTLDEKAKTLNNENGQSFIENLGLAMEQVYTN
                     ERGLLEQSTLQDRRKAFQFAYLSLMQEEKIQANHQITPDSIGLILGFLVERFMNNQEE
                     LHIVDIASGAGHLSATVKEVLPEIAVMHHLIEVDPVLSRVSVHLANFLEIPFDVYPQD
                     AIMPLPLEEADIVIGDFPVGYYPIDERSKEFKLGFEEGHSYSHYLLIEQAINALKDAG
                     YAFLVVPSNIFTGEHVKQLEKYIATETEMQAFLNLPPTLFKNEKARKSILILQKKKSG
                     ETKPVEVLLANIPDFKIPSQFQGFMTELNQWMDTNRPKK"
     gene            1781..2983
                     /locus_tag="O2701_002009"
     CDS             1781..2983
                     /locus_tag="O2701_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCZ4293892.1"
                     /translation="MSKLILAINAGSSSLKFQLIRMPEEELVTKGLVERIGLKDSIFT
                     IEVNGEKVKTVQDIKDHVEAVDIMLDAFKAHNIINDINDIDGTGHRVVHGGEKFPESV
                     AITDEVEKEIEELSELAPLHNPANLMGIRAFRKLLPNIPHVAIFDTAFHQTMPEKAYL
                     YSLPYHYYKDYGIRKYGFHGTSHKFVSQRAAEMLDKPIEDLRIISCHIGNGASIAAID
                     GGKSIDTSMGFTPLAGVTMGTRSGNIDPALIPFIMEKTGKTAEQVLEILNKESGLLGL
                     SGTSSDLRDLSEEAESGKARSQMALDVFASKIHKYIGSYAARMHGVDVIVFTAGIGEN
                     SVEIRAKVLEGLEFMGVYWDPKKNENLLRGKEGFINYPHSPVKVVVIPTDEESMIARD
                     VMTFGGLK"
     gene            complement(3229..3729)
                     /locus_tag="O2701_002010"
     CDS             complement(3229..3729)
                     /locus_tag="O2701_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCZ4293893.1"
                     /translation="MITYKNILIAVDGSHEAEWAFNRAVGVAKRNDAKLTIVNVIDSR
                     TYSSYEVYDAQFTEKSKHFAEELLNGYKEVATNAGVKDVETRLEFGSPKSIIPKKLAH
                     EINADLIMSGTSGLNAVERFIVGSVSESIVRHAPCDVLVVRTEELPADFQPQVATTQL
                     REKYQN"
     gene            3870..4988
                     /gene="ald"
                     /locus_tag="O2701_002011"
     CDS             3870..4988
                     /gene="ald"
                     /locus_tag="O2701_002011"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HCZ4293894.1"
                     /translation="MKIGIPREIKNNENRVGLSPSGVHALVESGHTVLVETNAGSGSF
                     FEDVDYKEAGAEIVAEQAKVWDVDMVIKVKEPLESEYPYFKEGLVLFTYLHLANEEKL
                     TQALIDRKVISIAYETVQLPDRSLPLLSPMSEVAGRMSAQVGAEFLQKLNGGMGILLG
                     GVPGVPKGKVTIIGGGQAGTNAAKIALGLGADVTILDVNPKRLQQLDDLFGGRVHTIM
                     SNPLNIELYVKQSDLVIGAVLIPGAKAPRLVTEDMIKQMKNGSVIIDIAIDQGGIFET
                     TDKITTHDDPTYIKHGVVHYAVANMPGAVPRTSTLALNNATLPYALMLANKGYREAFK
                     SNQPLSLGLNTYKGHVTNKGVAEAFEMEYKSVEEALQL"
     gene            5112..5210
                     /locus_tag="O2701_002012"
     CDS             5112..5210
                     /locus_tag="O2701_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293895.1"
                     /translation="MNDIDLIHYMLLMNIPAYVFLACLLHIAFRLG"
     gene            complement(5304..6359)
                     /locus_tag="O2701_002013"
     CDS             complement(5304..6359)
                     /locus_tag="O2701_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCZ4293896.1"
                     /translation="MTKISKIIDELNNQQADAAWITTPLNVYYFTGYRSEPHERLFAL
                     LIKKDGKQVLFCPKMEVEEVKASPFTGEIVGYLDTENPFSLYPQTINKLLIESEHLTV
                     ARQKQLISGFNVNSFGDVDLTIKQLRNIKSEDEISKIRKAAELADKCIEIGVSYLKEG
                     VTECEVVNHIEQTIKQYGVNEMSFDTMVLFGDHAASPHGTPGDRRLKSNEYVLFDLGV
                     IYEHYCSDMTRTIKFGEPSKEAQEIYNIVLEAETSAIQAIKPGIPLKDIDHIARNIIS
                     EKGYGEYFPHRLGHGLGLQEHEYQDVSSTNSNLLEAGMVITIEPGIYVPGVAGVRIED
                     DILVTNEGYEVLTHYEK"
     gene            6534..7223
                     /locus_tag="O2701_002014"
     CDS             6534..7223
                     /locus_tag="O2701_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCZ4293897.1"
                     /translation="MKLSFHGQSTIYLEGNNKKVIVDPFISNNPKCDLNIETVQVDYI
                     VLTHGHFDHFGDVVELAKKTGATVIGSAEMADYLSSYHGVENVHGMNIGGKANFDFGS
                     VKFVQAFHSSSFTHENGIPVYLGMPMGIVFEVEGKTIYHTGDTGLFSDMSLIAKRHPV
                     DVCFVPIGDNFTMGIDDASYAINEFIKPKISVPIHYDTFPLIEQDPQQFKDAVNVGDV
                     QILKPGESVQF"
     gene            complement(7528..7941)
                     /locus_tag="O2701_002015"
     CDS             complement(7528..7941)
                     /locus_tag="O2701_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCZ4293898.1"
                     /translation="MYKNILLGVDTQLKNEKALKEVSKLAGEGTVVTVLNAISEQDAQ
                     ASIKAGVHLNKLTEERSKRLEKTRKALEDYGIDYDQIIVRGNAKEELLKHANSGKYEI
                     VVLSNRKAEDKKKFVLGSVSHKVAKRATIPVLIVK"
     gene            8146..9444
                     /locus_tag="O2701_002016"
     CDS             8146..9444
                     /locus_tag="O2701_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCZ4293899.1"
                     /translation="MTKHEQILDYIESLSIGSKISVRKIAKFLNVSEGTAYRAIKDAD
                     KMGMVATIDRVGTVRIEKRNRNEIEHLTFNEIVNIIDGQVLGGNKGITKMVSKFAIGA
                     MELKDILKYIGPKTLLIVGNREDVQIEALKRGTAILITGGFKPSNKVINFANEHDLPV
                     LSSSYDTFLVANIINKALFNQKIRKDILIVQDIMTPLDDLSVLFDTMKIADYKRMANR
                     TGHTRFPVVNESYKLVGIVTSREMINTKDDDEIDKVMTRNPIYVNAMSTVASCAHMMI
                     WEGIELIPVVSSNKKTVGVINRQDVLKSMQLLGRQPQMGETINDQIAKYITMNQDGIT
                     VEVSPLLINHYGTVSKAAFVSIIEETIQYEMRKFKKGNVMIENLNIVYIKTVPIESHI
                     TVRFGILDVGRNFAKIEVNMHSQNDKVASALVICQMFDEV"
     gene            9705..10646
                     /gene="pde2"
                     /locus_tag="O2701_002017"
     CDS             9705..10646
                     /gene="pde2"
                     /locus_tag="O2701_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pApA hydrolase Pde2"
                     /protein_id="HCZ4293900.1"
                     /translation="MISTLNEIMKCIEDNDTIIIHRHVRPDPDAYGSQLGLKYYIQQK
                     FPQKQVFAVGEAESSLSFIGELDNIDDKTYQDALVIVCDTANAPRIDDERYSTGRKLI
                     KIDHHPAVDQYGDINLVNTNASSTSEIIYDLISHFNDEAIVNKDIASVLYLGIVGDTG
                     RFLFNNTSEHTMEIAGKLIGHDIDHNALLNKMMEKAPKMLPFQGYVLQHFELMDDGFC
                     QVKITEDVLEQFGIQPNEASQFVNTIADIKGLKIWVFAVDEGNEIRCRLRSKGQLIIN
                     DIAQDFGGGGHPNASGVSVDSWDEFEQLATALRTKLN"
     gene            10667..13864
                     /locus_tag="O2701_002018"
     CDS             10667..13864
                     /locus_tag="O2701_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCZ4293901.1"
                     /translation="MVAYLNIHTAYDLLNSSLKIEDAVRLAVSENVDALAITDTNVLY
                     GFPKFYDACIANNIKPIFGMTIYVTNGLNTVETVVLAKNNDGLKDLYQLSSEIKMNAL
                     EHVSFELLKRFSNNMIIIFKKVGDQHRDIVQVFETHNDTYMDHLSISIQGRKHVWIQN
                     VCYQTRQDADTISALAAIRDNTKLDLIHDQEDFGAHFLTEKEINQLDINQEYLTQVDV
                     IAQKCDAELKYHQSLLPQYETPNDESAKKYLWRVLVTQLKKLELNYDVYLERLKYEYK
                     VITNMGFEDYFLIVSDLIHYAKTNDVMVGPGRGSSAGSLVSYLLGITTIDPIKFNLLF
                     ERFLNPERVTMPDIDIDFEDTRRERVIQYVQEKYGELHVSGIVTFGHLLARAVARDVG
                     RIMGFDEVTLNEISSLIPHKLGITLDEAYQIDDFKKFVHRNHRHERWFSICKKLEGLP
                     RHTSTHAAGIIINDHPLYEYAPLTKGDTGLLTQWTMTEAERIGLLKIDFLGLRNLSII
                     HQILTQVKKDLGINIDIEKIPFDDQKVFELLSQGDTTGIFQLESDGVRSVLKKLKPEH
                     FEDIVAVTSLYRPGPMEEIPTYITRRHDPSKVQYLHPHLEPILKNTYGVIIYQEQIMQ
                     IASTFANFSYGEADILRRAMSKKNRAVLESERQHFIEGAKQNGYHEDISKQIFDLILK
                     FADYGFPRAHAVSYSKIAYIMSFLKVHYPNYFYANILSNVIGSEKKTAQMIEEAKKQG
                     ITILPPNINESHWFYKPSQEGIYLSIGTIKGVGYQSVKVIVDERYQNGKFKDFFDFAR
                     RIPKRVKTRKLLEALILVGAFDAFGKTRSTLLQAIDQVLDGDLNIEQDGFLFDILTPK
                     QMYEDKEELPDALISQYEKEYLGFYVSQHPVDKKFVAKQYLTIFKLSNAQNYKPILVQ
                     FDKVKQIRTKNGQNMAFVTLNDGIETLDGVIFPNQFKKYEELLSHNDLFIVSGKFDHR
                     KQQRQLIINEIQTLATFEEQKLAFAKQIIIRNKSQIDMFEEMIKATKENANDVVLSFY
                     DETIKQMTTLGYINQKDSMFNNFIQSFNPSDIRLI"
BASE COUNT         4785 a         2014 c         2552 g         4524 t
ORIGIN      
        1 tgcttttgat tatattcaac aatttgtata aaattaaatt tgcttttgat taaagcatga
       61 aaattgtaat caaaccataa attgtcgtat gatgtagtta gaattttaaa atgcaggagg
      121 tcaagtatat gactgaaata acattcaaag gtggaccaat ccacttaaaa ggtcaacaaa
      181 ttaatgaagg tgattttgca cctgatttta cagtgttaga taatgactta aatcaagtaa
      241 cattagcaga ttatgctggt aaaaagaaat taattagtgt ggtaccatca attgatacag
      301 gtgtttgtga tcagcagact cgcaaattca actctgatgc ttctaaagaa gaggggattg
      361 tgcttacaat ttcagcagac ttaccattcg cacaaaaaag atggtgcgct tcagcaggtt
      421 tagacaatgt cattacatta agtgaccacc gtgacttatc atttggtgaa aactatggcg
      481 ttgttatgga agaacttcgc ttattagctc gtgcagtatt tgtattagat gcagataata
      541 aagttgttta taaagaaatc gttagtgaag gtactgattt cccagatttt gatgctgctt
      601 tagctgcata caaaaatatt taatcattaa agagataaat cttaaaatgt atacatcgtg
      661 tccatcgttg tcaacagcat taaaatagaa ttgttttcta tgattgctaa gacctatggg
      721 cactttttat tggagaggga cgaatatggc agaacaacaa acaattatgg aacgcttgtt
      781 tcatacatta gatgaaaaag ctaaaacatt aaataatgaa aatggccaaa gttttattga
      841 aaatcttggg ctagcaatgg aacaagtata taccaatgaa agaggattgt tagaacaatc
      901 gacgttacaa gatcgacgta aagcattcca atttgcatat ttaagtttaa tgcaggaaga
      961 aaagatacaa gcaaatcatc aaattacacc agattcaatt ggattgatac taggattttt
     1021 agttgagcgt tttatgaaca accaagaaga attacatatt gttgatattg caagtggtgc
     1081 cggtcattta agtgctactg taaaagaagt gttacctgaa attgcggtta tgcatcattt
     1141 aattgaagtt gatccagttt tatcacgtgt tagtgtacat ttagcaaact tcttagaaat
     1201 tcctttcgat gtgtatcctc aagatgccat catgccacta ccattagaag aagcagatat
     1261 cgttattggt gattttccag taggctatta tccaattgat gaaagaagta aggagtttaa
     1321 gctaggtttt gaagaaggac atagttattc acattattta ttaatagaac aagcaataaa
     1381 tgcattaaaa gatgctggat atgcctttct agtggtacca agtaatattt ttacaggtga
     1441 acatgtaaaa cagcttgaaa aatatattgc aacagagaca gagatgcaag catttttaaa
     1501 tttaccacca actttattta aaaatgaaaa agcgcgaaaa tctatattaa ttttacaaaa
     1561 gaaaaaatcg ggtgaaacaa agccagttga agtattattg gcaaatattc ctgatttcaa
     1621 aattccttca caatttcaag gatttatgac agagttaaat cagtggatgg acacaaatcg
     1681 tcctaaaaaa taatctgtaa tagtatagtc ataaaactgt atgaatgata aaatgaaaaa
     1741 tgaaatagta taaaaagcaa acgatggagg atcattactt atgtcaaaat taatcttggc
     1801 tatcaatgct ggtagttcat cattaaaatt tcaattaatt agaatgcctg aagaggaatt
     1861 agtaacaaaa ggtttagtag aaagaatcgg attgaaggat tcaattttta caattgaagt
     1921 caacggggaa aaagttaaaa cagtacaaga tatcaaggat cacgttgagg cagttgatat
     1981 catgttggat gcttttaaag cgcacaatat tattaatgat attaacgata tcgatggaac
     2041 aggtcaccgt gttgtacatg gtggtgaaaa attccctgaa tcagtagcta ttactgatga
     2101 ggttgaaaag gaaattgaag aattaagcga attagcaccg cttcacaacc cagctaactt
     2161 gatgggtatt cgtgctttcc gtaaattatt accaaatatc cctcatgtgg caatttttga
     2221 tacagcattc catcaaacaa tgcctgaaaa agcatatcta tatagcttgc catatcatta
     2281 ttataaagat tatggcattc gtaagtatgg tttccatggt acaagccata aatttgtatc
     2341 acaaagagcg gcagaaatgt tagataagcc aattgaagat ttacgtatta tttcatgtca
     2401 cattggtaat ggagcatcaa ttgcagctat cgatggcggt aaatctattg atacatcaat
     2461 gggcttcaca ccacttgctg gtgtaacaat gggtactcgt tctggtaata ttgaccctgc
     2521 tttaattcca tttattatgg aaaaaacagg taaaactgct gaacaagtat tggaaatctt
     2581 aaacaaagaa tcaggtttat taggattatc aggtacatct agtgatttac gtgacttatc
     2641 agaagaagct gaatcaggta aagcacgttc acaaatggct ttagatgtat ttgcttctaa
     2701 aattcacaaa tatattggat catatgctgc gagaatgcat ggtgttgatg ttatcgtatt
     2761 tacagcaggt atcggtgaaa actcagtaga aattcgtgcg aaagttcttg aaggcttaga
     2821 atttatgggt gtttactggg atcctaagaa aaatgaaaac ttactacgtg gtaaagaagg
     2881 atttattaac tatcctcatt caccagttaa agttgtcgtt attccaacag atgaagaaag
     2941 tatgattgcc cgtgatgtta tgacattcgg tggtttaaaa taatttttaa ataaatatta
     3001 agcgtctatc tccgctacaa ttatatgtag gaggtagacg ctttttgtat tgtttgaaaa
     3061 ttaactagtg taatgtctct tttcacgaat atcttaaaga attaagtagt cgaagaagat
     3121 tatgaaaaca ttattaagtg agttttattt ataaacaata aaaaaccaag tgcagaaagt
     3181 tgcacttggt tcataatgat caagtagtta tctaatgatc atctatattt aattttgata
     3241 tttttcacgt aattgagttg ttgcaacttg tggttggaag tctgctggta actcttcagt
     3301 acgaacaact aacacgtcac atggcgcatg acgaacgata gattctgata cagaaccaac
     3361 aataaatctt tccacggcat ttaagcctga tgtaccactc ataatcaagt ctgcattaat
     3421 ttcatgtgca agctttttag gaatgataga tttaggagag ccaaactcta gacgcgtttc
     3481 tacatcttta acaccagcgt tagtagctac ttctttataa ccatttaata attcttctgc
     3541 aaaatgctta gatttttcag taaattgagc atcataaact tcataagaag aatacgttct
     3601 tgaatcaatt acattcacaa ttgttaactt cgcatcgtta cgtttagcaa caccaactgc
     3661 tctgttaaat gcccattccg cttcatgtga accgtcaact gcgattaaaa tatttttgta
     3721 agtaatcata gtaagtgcct ccttgttctt tcttaattat attttaccac aattttcgga
     3781 aaagtcagtg taataacctt tacaattttg tggatttttg attgcgcttt caaaatgttc
     3841 gcgttaagat gattatggag gtggatggta tgaaaattgg tataccaagg gagattaaaa
     3901 ataatgaaaa tcgtgttggt ttatcaccaa gtggtgtgca cgctttagta gaaagtgggc
     3961 atactgtttt agtggaaaca aatgcgggtt caggatcatt ctttgaagat gtagattaca
     4021 aagaagcagg tgctgagatt gttgctgaac aagcaaaagt ttgggatgtg gatatggtta
     4081 ttaaagttaa agaaccactt gaatctgaat atccatattt taaagaaggg cttgtattat
     4141 tcacttatct tcatttagca aatgaagaaa aattaacaca agctttgata gatagaaaag
     4201 taattagtat tgcatatgag actgtgcagt taccagaccg atctttacca ttgttatcac
     4261 caatgagtga ggtagcagga agaatgtcag ctcaagttgg cgcagagttc ctacaaaaac
     4321 ttaatggtgg tatgggaatt ctactaggtg gtgtcccagg agtacctaag ggtaaagtaa
     4381 ctattatcgg tggtggtcaa gcaggaacaa atgcagctaa aattgcacta ggactaggtg
     4441 cagatgttac gattttagat gttaatccaa agcgtttaca acaattagat gatttattcg
     4501 gtggacgtgt acatacaatt atgtcaaatc cgttgaatat tgagttgtat gttaaacaaa
     4561 gtgatttagt aataggtgca gttttaattc caggtgctaa agcgccaaga cttgtaacag
     4621 aagacatgat taaacaaatg aaaaatgggt cagttattat tgacattgct attgatcaag
     4681 gcggtatttt tgaaacaact gataaaatta cgacacatga tgatcctaca tatattaagc
     4741 atggtgtggt tcattatgca gttgcaaata tgccaggtgc agtaccgcgt acttcgacgt
     4801 tagctttaaa taatgctacg ctaccttatg cgctcatgct agctaataaa gggtatagag
     4861 aagcatttaa atcaaatcaa ccattatcat taggtttaaa tacttacaaa ggtcacgtaa
     4921 ccaataaagg cgttgcagag gcatttgaaa tggaatataa atctgtagaa gaagcattac
     4981 aattataatt tgaatttgat gaaaagggaa tggattacag cgtactaaag aatgacgtta
     5041 tcatctcctt ggttattaaa attaaataat gataaattaa agctagaaac aatgcgaaga
     5101 aatcatcgaa catgaatgat attgatttga ttcattacat gttactgatg aacatacctg
     5161 catatgtgtt tctagcttgt ttgttacata ttgcatttag acttggataa aaatttatgt
     5221 cttaggcgct acaatgttat attggcagtg tttgactgaa tgagaatgcg cttgtaacaa
     5281 gcttttcatt tttatcccac tccttatttt tcgtaatgtg ttaatacttc atatccttca
     5341 ttagtgacaa gtatgtcatc ttcaattctt acacctgcaa caccaggtac ataaatacct
     5401 ggttcgattg taataaccat gccagcttct aacaaattag aattagtact tgaaacatct
     5461 tgatattcat gttcttgtaa tcctaggcca tgacctaagc gatgagggaa atattcacca
     5521 taaccttttt ctgaaataat atttctagcg atatgatcga tatcttttaa tggtattcca
     5581 ggtttaattg cttggattgc agatgtttct gcttctaata caatattata aatttcttgt
     5641 gcttctttgc taggttcacc aaatttaata gtacgtgtca tatcgctaca ataatgctca
     5701 taaattacac ctaaatcaaa tagtacatat tcattgcttt ttaatctgcg atctcctggt
     5761 gtgccatgag gtgatgcggc atgatctcca aataaaacca tcgtatcaaa actcatttca
     5821 ttgacgccat attgtttgat agtttgctca atatggttga ctacttcaca ttcagtcaca
     5881 ccttctttta aataagaaac acctatttcg atacacttat ctgctaactc agcagcttta
     5941 cgtattttgc taatttcatc ttcggattta atatttctca attgtttgat tgttaaatca
     6001 acatctccga atgaattgac attgaaacca gagattaatt gtttttggcg tgctactgtt
     6061 aagtgctcgc tttcaattag taatttattg attgtttgag gataaagtga aaaagggttt
     6121 tcagtgtcta aatatccaac gatttcacct gtgaaaggtg atgctttgac ttcttcgact
     6181 tccatttttg gacaaaatag tacttgttta ccatctttct taatcaataa tgcaaataat
     6241 ctttcatggg gttcgctacg gtatccagta aaataatata cattcaacgg tgttgtaatc
     6301 catgctgcat cagcttgttg attgttcaat tcgtctatta tttttgatat ttttgtcatt
     6361 ttataaacac tcctcaacat aatattactt ccattttatg ccaattatta ttagatttca
     6421 aaatcgagct aatacattaa ctagattttc aatttttaaa atttgagaat ttgttataat
     6481 taagtggcaa aagggtacta aatttatata gtaattgata ggaggatgct caaatgaagt
     6541 tatcatttca tggtcaatca acgatttatt tagaagggaa caacaaaaag gttatcgtag
     6601 atccgtttat ttcaaataac cctaagtgtg atttgaatat tgaaacagtg caggtagact
     6661 atatcgtttt aacgcacggt cattttgatc attttggtga tgttgtggaa ctagcaaaga
     6721 aaacaggagc aacagtaatt ggaagtgcag aaatggctga ctatctttct tcatatcacg
     6781 gtgttgaaaa tgttcatggt atgaatattg gtggtaaagc aaactttgat ttcggaagcg
     6841 ttaaatttgt tcaagcattt catagttcaa gttttacaca tgaaaatggt attccagttt
     6901 atttaggtat gccaatgggt atcgtgtttg aagttgaagg aaaaacaatt tatcatactg
     6961 gtgatacagg tctgtttagt gacatgtcac ttattgcgaa acgtcatcca gttgatgtat
     7021 gttttgtacc aattggagat aattttacga tgggtattga tgatgcaagt tatgctatta
     7081 atgaatttat taaaccaaaa atttctgtac cgattcatta tgatacattc ccattaatcg
     7141 aacaagatcc acaacaattt aaagatgctg ttaatgtagg ggacgtgcaa attttaaaac
     7201 caggtgaatc tgttcagttt taaatagaat atagatttta cactaaaaaa cacctcgcta
     7261 atctatgtgg aagttatcgt ctgcatattt ttagtgggtg tttttatatt ctactgattc
     7321 agttacgacg tggacggggg acatcagtgg ttctttatca tttctgacct actctcgtaa
     7381 ttttagtttt atacaattag acattgatta taaatttact acgatataaa aaatccagaa
     7441 ccacagtcat aatcgtttct tgtatttact cagtcgtttg agactgcatc atgattgaaa
     7501 gattatttgt gattctggat aaaaatttta tttaacgatt aatacaggga tagtcgcacg
     7561 ttttgctact ttgtggctga cacttccaag tacaaatttc tttttgtctt ctgctttacg
     7621 gttacttaaa acaacaattt cgtatttacc gctattagca tgttttaata gttcttcttt
     7681 tgcattacca cgaacaatta tttggtcata atcaatacca taatcttcta aagctttgcg
     7741 tgttttttcc aatcgcttgc ttcgttcttc agtaagtttg tttaaatgaa cacctgcttt
     7801 aattgatgct tgagcatctt gttcgctgat tgcgtttaaa actgttacga ctgtaccttc
     7861 gccagctaat ttagacactt cttttagtgc tttttcattt tttaactgag tgtctacacc
     7921 aagtaatata tttttataca ttagtacatc cctcctcact tacattctaa taaataataa
     7981 tggatttttc catttaaaat taaagatttt gtgatctttg tgtttgttgt atattttcaa
     8041 aaaataaaat aggtgaaacg atcattattt catttcactt caatgctttt tatccaaaat
     8101 aaagttataa tgtagttaat actgttcgaa aagaagaggt aaagaatgac aaaacatgaa
     8161 caaattttag attatattga atcgttatct ataggctcta aaatttcggt gcgtaaaatc
     8221 gctaaatttt taaacgtatc tgaagggaca gcatatcgtg caattaaaga tgctgataaa
     8281 atgggcatgg tcgcaacgat agatcgtgtt ggtacggttc gaatcgaaaa acgtaatcgt
     8341 aatgaaattg aacatttaac ttttaatgaa attgtcaata ttattgatgg ccaagtgtta
     8401 ggtggcaaca aaggtatcac taagatggta tctaaatttg caattggagc aatggaactc
     8461 aaagatattt taaagtatat tggtccaaag acattattga ttgtcggaaa tcgtgaagat
     8521 gtgcagattg aagctttaaa aagaggtacg gctatattaa ttactggtgg atttaagcca
     8581 tctaataaag ttattaattt tgcgaatgaa catgatttac ctgttctatc atcaagttat
     8641 gatacattcc tagttgcaaa tattatcaac aaagcacttt ttaatcagaa aatacgaaaa
     8701 gatatcttaa tagttcaaga tataatgacg ccattagatg atttatctgt gctgtttgat
     8761 acgatgaaaa tagcagatta taagcgtatg gcgaatcgaa ctggccatac aagatttccg
     8821 gtagtcaatg agtcgtataa attagtaggt attgtaacaa gtcgagagat gattaataca
     8881 aaggacgacg acgaaataga taaagtgatg acgcgaaatc ctatttatgt aaatgctatg
     8941 agtacagtag caagttgtgc gcatatgatg atttgggaag gtattgaatt gattcctgtc
     9001 gtttcgagta ataaaaagac agtgggtgtg attaatagac aagatgtttt aaagtctatg
     9061 caacttttag gaagacagcc tcagatgggt gaaacaatta acgatcaaat cgccaaatat
     9121 ataacaatga atcaagatgg aattacagta gaagtttcac cacttttaat caatcattac
     9181 ggaacagtaa gtaaagcggc atttgtaagt attatagaag aaacaataca atatgaaatg
     9241 cgtaaattta aaaaaggtaa tgtcatgata gagaacttaa atatcgtgta tattaaaaca
     9301 gtacctattg aatcacatat aacagtgcgc tttggcatac tagacgtcgg aagaaacttc
     9361 gccaaaatag aagtgaacat gcatagtcaa aacgacaaag tagcgagcgc tctcgtgata
     9421 tgccaaatgt ttgacgaagt ataggaactt agcagagaaa ataggcataa gatagatttc
     9481 gcacaaacgt tggggcccca ccccaacttg cattgtttgt agaaattagg ggtccaattt
     9541 ctctgtgttg gggcccctga actaaatagt atgaaaatat atatatctaa ataattaaaa
     9601 cgggcaaata gtttaatatg gtagtaacaa tttttataat ttagatattt tgatatagac
     9661 taatgcttaa attaagcaac ttttctaaaa aagggagtat gaaaatgatt agtactttga
     9721 atgaaattat gaaatgtatc gaagacaacg atacaattat tatacacaga catgttagac
     9781 cagatcctga tgcatatggt tcacaacttg gtttaaaata ctatattcag cagaaatttc
     9841 cgcaaaagca agtatttgca gtgggtgaag cggaatcatc attaagtttt attggagagt
     9901 tagataacat tgatgataaa acatatcaag atgcgcttgt aattgtatgt gatactgcca
     9961 atgctccacg aattgacgat gaacgttata gtacaggtcg taaacttatt aaaatagatc
    10021 atcatcctgc agttgatcag tatggtgata ttaatttagt taatacgaac gcgtcatcta
    10081 caagtgaaat catttacgat ttaatctcac attttaatga tgaagcaatt gttaataaag
    10141 atatcgcgag cgttttatat cttggtatcg tcggtgatac tgggcgattc ctttttaaca
    10201 atacctcaga acatactatg gaaattgctg gaaagttaat tgggcatgat attgatcata
    10261 atgcgctttt aaataaaatg atggagaagg ccccaaaaat gttgccgttt caaggttatg
    10321 ttttacaaca tttcgaactt atggatgatg gattctgcca agttaaaata actgaagatg
    10381 tattggagca attcggtatt cagccaaatg aagcatctca gtttgttaat acaattgctg
    10441 acatcaaagg cttgaaaata tgggtatttg cagtcgatga aggtaatgaa atcagatgtc
    10501 gattacgttc taaagggcaa ttgattatta atgatattgc gcaagatttt ggtggcggtg
    10561 gtcatccgaa tgcgtcagga gtttcagtgg acagctggga tgaatttgag caacttgcta
    10621 cagctttacg cacaaaactt aactaataga aaggggccat tcaatcatgg tggcatattt
    10681 aaatattcat acggcttatg atttgttaaa ttcaagctta aaaatagaag atgccgtaag
    10741 acttgctgtg tctgaaaatg ttgatgcact tgccataact gacaccaatg tattgtatgg
    10801 ttttcctaaa ttttatgatg catgtatagc aaataacatt aaaccgattt ttggtatgac
    10861 aatatatgtg acaaatggat taaatacagt cgaaacagtt gttctagcta aaaataatga
    10921 tggattaaaa gatttgtatc aactatcatc ggaaataaaa atgaatgcat tagaacatgt
    10981 gtcgtttgaa ttattaaaac gattttctaa caatatgatt atcattttta aaaaagtcgg
    11041 tgatcaacat cgtgatattg tacaagtgtt tgaaacccat aatgacacat atatggacca
    11101 ccttagtatt tcgattcaag gtagaaaaca tgtttggatt caaaatgttt gttaccaaac
    11161 acgtcaagat gccgatacga tttctgcatt agcagctatt agagacaata caaaattaga
    11221 cttaattcat gatcaagaag attttggtgc acatttttta actgaaaagg aaattaatca
    11281 attagatatt aaccaagaat atttaacgca ggttgatgtt atagctcaaa agtgtgatgc
    11341 agaattaaaa tatcatcaat ctctacttcc tcaatatgag acacctaatg atgaatcagc
    11401 taaaaaatat ttgtggcgtg tcttagttac acaattgaaa aaattagaac ttaattatga
    11461 cgtctattta gagcgattga aatatgagta taaagttatt actaatatgg gttttgaaga
    11521 ttatttctta atagtaagtg atttaatcca ttatgcgaaa acgaatgatg tgatggtagg
    11581 tcctggtcgt ggttcttcag ctggctcact ggtcagttat ttattgggaa ttacaacgat
    11641 tgatcctatt aaattcaatc tattatttga acgtttttta aacccagaac gtgtaacaat
    11701 gcctgatatt gatattgact ttgaagatac acgccgagaa agggtcattc agtacgtcca
    11761 agaaaaatat ggcgagctac atgtatctgg aattgtgact ttcggtcatc tgcttgcaag
    11821 agcagttgct agagatgttg gaagaattat ggggtttgat gaagttacat taaatgaaat
    11881 ttcaagttta atcccacata aattaggaat tacacttgat gaagcatatc aaattgacga
    11941 ttttaaaaag tttgtacatc gaaaccatcg acatgaacgc tggttcagta tttgtaaaaa
    12001 gttagaaggt ttaccaagac atacatctac acatgcggca ggaattatta ttaatgacca
    12061 tccattatat gaatatgccc ctttaacgaa aggggataca ggattattaa cgcaatggac
    12121 aatgactgaa gccgaacgta ttgggttatt aaaaatagat tttctagggt tgagaaactt
    12181 atcgattatt catcaaatct taacacaagt caaaaaagat ttaggtatta atattgatat
    12241 cgaaaagatt ccgtttgatg atcaaaaagt gtttgaattg ttgtcgcaag gagatacgac
    12301 tggcatattc caattagagt ctgacggtgt aagaagtgta ttaaaaaaat taaagccgga
    12361 acactttgaa gatattgttg ctgtaacttc tttgtataga ccaggtccaa tggaagaaat
    12421 tccaacttac attacaagaa gacatgatcc aagcaaagtt caatatttac atccgcattt
    12481 agaacctata ttaaaaaata cttacggtgt tattatttat caagagcaaa ttatgcaaat
    12541 agcgagcaca tttgcaaact tcagttatgg tgaagcggat attttaagaa gagcaatgag
    12601 taaaaaaaat agagctgttc ttgaaagtga gcgtcaacat tttatagaag gtgcaaagca
    12661 aaatggttat cacgaagaca ttagtaagca aatatttgat ttgattctga aatttgctga
    12721 ttatggtttt cctagagcac atgctgtcag ctattctaaa attgcataca ttatgagctt
    12781 tttaaaagtc cattatccaa attattttta cgcaaatatt ttaagtaatg ttattggaag
    12841 tgagaagaaa actgctcaaa tgatagaaga agcaaaaaaa caaggtatca ctatattgcc
    12901 accgaacatt aacgaaagtc attggtttta taaaccttcc caagaaggca tttatttatc
    12961 aattggtaca attaaaggtg ttggttatca aagtgtgaaa gtgattgttg atgaacgtta
    13021 tcagaacggc aaatttaaag atttctttga ttttgctaga cgtataccga agagagtcaa
    13081 aacgagaaag ttacttgaag cactgatttt agtgggagcg tttgatgctt ttggtaaaac
    13141 acgttcaacg ttgttgcaag ctattgatca agtgttggat ggcgatttaa acattgaaca
    13201 agatggtttt ttatttgata ttttaacgcc aaaacagatg tatgaagata aagaagaatt
    13261 gcctgatgca cttattagtc agtacgaaaa agaatattta ggattttatg tttcgcaaca
    13321 cccagtagat aaaaagtttg ttgccaaaca atatttaacg atatttaaat tgagtaacgc
    13381 gcagaattat aaacctatat tagtacagtt tgataaagtt aaacaaattc gaactaaaaa
    13441 tggtcaaaat atggcattcg tcacattaaa tgatggcatt gaaactttag atggtgtgat
    13501 tttccctaat cagtttaaaa agtacgaaga gttgttatca cataatgact tgtttatagt
    13561 tagcgggaaa tttgaccata gaaagcaaca acgtcaacta attataaatg agattcagac
    13621 attagccact tttgaagaac aaaaattagc atttgccaaa caaattataa ttagaaataa
    13681 atcacaaata gatatgtttg aagagatgat taaagctacg aaagagaatg ctaatgatgt
    13741 tgtgttatcc ttttatgatg aaacgattaa acaaatgact actttaggct atattaatca
    13801 aaaagatagt atgtttaata attttataca atcctttaac cctagtgata ttaggcttat
    13861 ataactttta tcaac
//
LOCUS       DALAGD010000079        13554 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.080, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000079 DALAGD010000000
VERSION     DALAGD010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13554
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.080"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(<1..427)
                     /locus_tag="O2701_002019"
     CDS             complement(<1..427)
                     /locus_tag="O2701_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCZ4293902.1"
                     /translation="MKIISKYILTRFSKNGKHFDIPKEQRRVIPKFKNRRKMIVICFY
                     SLLAILLILLLASFIKATRANNDSKEAVNKTNMIQKKYEDNANTVQYSPKLKLYADKF
                     IDTYMNISKDSKELESREKELLKYFPSDYKKPDEKISDTE"
     gene            complement(488..1579)
                     /locus_tag="O2701_002020"
     CDS             complement(488..1579)
                     /locus_tag="O2701_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000172946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCZ4293903.1"
                     /translation="MTLKNQCTPLTNRGVERTNKSAVEAVVDWVQVTFHIAPISAVIE
                     DVIGLPITLFKKRNSGIYFYNRGYEFSNIKLYYSSDDESMGIHLQLTGTGCREFEHHL
                     QQLNKTWQDFFDKCLSVNANFTRIDIAIDDYKTYLKVPLLIKKAEKAECVSRFRAGSA
                     INGFNLSDGRSKGATFYIGSKQSNLYCRFYEKNYEQAFKRHCDVEDIGLWNRYEIQMR
                     KAYAVNCAKVLSRTDNISEIVKSILHNNLRFISPPKGGNDKNRKRWPLYRPWALFIKD
                     TEKLNLTTRPTLKSIEDNLDWLCKQVATTLDTVLTAESMAQSEGLLTDTDFLDKILAH
                     SQFNDEHTNRINHYLEALKQKKHLSKDKC"
     gene            complement(1754..2056)
                     /locus_tag="O2701_002021"
     CDS             complement(1754..2056)
                     /locus_tag="O2701_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293904.1"
                     /translation="MDYYTADRLYRYTNSSNLSEPILNYVASRINWGDKVSLMTLAKE
                     IQSKFNDSYVKENTVKGRPKIYADLCLLCMSLSEAGHGRMLQVNLEDCIYIGDIDV"
     gene            complement(2070..2390)
                     /locus_tag="O2701_002022"
     CDS             complement(2070..2390)
                     /locus_tag="O2701_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF961 family protein"
                     /protein_id="HCZ4293905.1"
                     /translation="MKPVLDIKKTLGQLNFLGVDEKYKYENGERTDKTVYAYKLASQE
                     QGEQITVKTPNKVELDYLQECELVEPDVKMYVQMSGDFGTIAYSWNAEDIKVVGSNSA
                     LKQK"
     gene            complement(2541..2825)
                     /locus_tag="O2701_002023"
     CDS             complement(2541..2825)
                     /locus_tag="O2701_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001581821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293906.1"
                     /translation="MTEFTPFFDGSNQLNYLKFVILRFHGVSKRKICKMYNFAYFRIL
                     EVCEMAKKNDYRFTYKDYKFLKQYGVSNTFICKMYHISIEDLEFFEVMNR"
     gene            complement(2834..3262)
                     /locus_tag="O2701_002024"
                     /pseudo
     CDS             complement(2834..3262)
                     /locus_tag="O2701_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574632.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            complement(3457..3681)
                     /locus_tag="O2701_002025"
     CDS             complement(3457..3681)
                     /locus_tag="O2701_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HCZ4293907.1"
                     /translation="MTKTLELSFKSSLDKPVKLQLPQLNNVVTEQVARDSMNALVDLN
                     IIKSNSGTITKVHSAQIIDKTTTVLFEDKK"
     gene            complement(3698..3901)
                     /locus_tag="O2701_002026"
     CDS             complement(3698..3901)
                     /locus_tag="O2701_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HCZ4293908.1"
                     /translation="MSKINHITIVLSFTKVDANGKQTEFKRRFANINPDASNDQIKTF
                     SKLIERLTGETYSNIELIKSLSI"
     gene            4060..4620
                     /locus_tag="O2701_002027"
     CDS             4060..4620
                     /locus_tag="O2701_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCZ4293909.1"
                     /translation="MNKVEAIKFNDDIVKMYEALKIKSERDYLFFKLAIHSGLKVSEL
                     LTITVSQVKRLIEKCTLSEMCKAHFHSLIKIRLPETLSKELLQYIEDRSLSNEDVLFQ
                     SLRTNQVLSRQQAYRIIHQASIEAGIDNVGLTTLRKTFAYHAYQKGIPIPVIQKYLGH
                     QSAIETLNFIGLENECEHSIYISLQL"
     gene            4639..7041
                     /gene="mnhA2"
                     /locus_tag="O2701_002028"
     CDS             4639..7041
                     /gene="mnhA2"
                     /locus_tag="O2701_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit A"
                     /protein_id="HCZ4293910.1"
                     /translation="MSLVYLLIAILVIMAMILLMSKRRALAKYAGYIALVAPVISSIY
                     FLIQIPSVAKLQYLSTSIPWIKTLDINLDLRLDGLSLMFSLIISLIGIAVFFYATQYL
                     SSRKDNLPRFYFYLTLFMFSMIGIVLSDNTILMYIFWELTSVSSFLLISYWYNNGDSQ
                     FGAIQSFMITVFGGLALLVGFIMLYIMTGTNNITEILGQADHIKNHGLFIPMIFMFLL
                     GAFTKSAQFPFHIWLPRAMAAPTPVSAYLHSATMVKAGIFLLLRFTPLLGLSNMYVYI
                     VTFVGLITMLFGSITALKQWDLKGILAYSTISQLGMIMAMVGIGGGYAQHQQDAIASI
                     YVFVLFGALFHLMNHAIFKCALFMGVGILDHEAGSRDIRILSGMRQLFPKMNLVMTIA
                     ALSMAGVPFLNGFLSKEMFLDALTQTGQLSQFSLISMIAIVFVGVIASVFTFTYALYM
                     VKEVFWTKYDSKVFTKKNIHEPWLFSLPSLILMVLVPVIFFVPNIFGKGIIVLALRAV
                     SGGNHQIDQLAPHVSQWHGFNIPLLLTIIIILLGSVLAIKVDWKKVFTGKIRQISVSK
                     SYEMVYRHFEKFATKRFKRVMQDRLNQYIIMTLGIFMIIIGYGYIRIGLPKVHQLHVS
                     EFGALEIILAIVTVTIGISLIFIRQRLTMVILNGVIGFVVTLFFIAMKAPDLALTQLV
                     VETITTILFIVSFSRLPNVPRSNANKKREIIKISVSLLMALIVVSLIFITQQTDGLSS
                     ISDFYLKADKLTGGKNIVNAILGDFRALDTLFEGLVLIITGLGIYTLLNYQDRRGQDE
                     RE"
     gene            7028..7453
                     /gene="mnhB2"
                     /locus_tag="O2701_002029"
     CDS             7028..7453
                     /gene="mnhB2"
                     /locus_tag="O2701_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit B"
                     /protein_id="HCZ4293911.1"
                     /translation="MKENDVVLRTVTKLVVFILLTFGFYVFFAGHNNPGGGFIGGLIF
                     SSAFILIFLAFNVEEVLESLPIDFRILMIIGALVSSITAIIPMFFGKPFLSQYETTWI
                     LPILGQIHVSTITLFELGILFSVVGVIVTVMLSLSGGRS"
     gene            7450..7794
                     /gene="mnhC2"
                     /locus_tag="O2701_002030"
     CDS             7450..7794
                     /gene="mnhC2"
                     /locus_tag="O2701_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit C"
                     /protein_id="HCZ4293912.1"
                     /translation="MNLILLLVIGFLVFIGTYMILSINLIRIVIGISIYTHAGNLIIM
                     SMGTYGSSRSEPLITGGNQLFVDPLLQAIVLTAIVIGFGMTAFLLVLVYRTYKVTKED
                     EIEGLRGEDDAK"
     gene            7784..9280
                     /gene="mnhD2"
                     /locus_tag="O2701_002031"
     CDS             7784..9280
                     /gene="mnhD2"
                     /locus_tag="O2701_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit D"
                     /protein_id="HCZ4293913.1"
                     /translation="MLSNLLILPMLLPFLCALILVFLKNNDRISKYLYLGTMTITTII
                     SLMLLIYVQRHRPITLDFGGWSAPFGIQFLGDSLSLIMVTTASFVITLIMAYGFGRGE
                     HKANRYHLPSFILFLSVGVIGSFLTSDLFNLYVMFEIMLLASFVLITLGQSVEQLRAA
                     IIYVVLNIIGSWLFLLGIGLLYKTVGTLNFSHIAMRLNDMGDNRTVTMISLIFLVAFS
                     AKAALVLFMWLPKAYAVLNTELAALFAALMTKVGAYALIRFFTLLFDQHNDLIHPLLA
                     TMAAITMVIGAIGVIAYKDIKKIAAYQVIISIGFIILGLGTNTFAGINGAIFYLVNDI
                     VVKTLLFFIIGSLVYITGYRQYQYLNGLAKKEPLFGVAFIIMIFAIGGVPPFSGFPGK
                     VLIFQGALQNGNYIGLALMIITSLIAMYSLFRILFYMYFGDKDGEEVNFKKIPLYRKR
                     ILSILVVVVIAIGIAAPVVLNVTSDATELNTSDQLYQKLVNPHLKGED"
     gene            9281..9763
                     /gene="mnhE2"
                     /locus_tag="O2701_002032"
     CDS             9281..9763
                     /gene="mnhE2"
                     /locus_tag="O2701_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit E"
                     /protein_id="HCZ4293914.1"
                     /translation="MNQIVLNIIIAFLWVLFQDEDHFKFSTFFSGYLIGLIVIYILHR
                     FFSDDFYVRKIWVAIKFLGVYLYQLITSSISTINYILFKTKDMNPGLLSYETRLTSDW
                     SITFLTILIIITPGSTVIRISQDSKKFFIHSIDVSEKEKDSLLRSIKHYEDLILEVSR
                     "
     gene            9760..10062
                     /gene="mnhF2"
                     /locus_tag="O2701_002033"
     CDS             9760..10062
                     /gene="mnhF2"
                     /locus_tag="O2701_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit F"
                     /protein_id="HCZ4293915.1"
                     /translation="MIQTITHIMIISSLIIFGIALIICLFRLIKGPTTADRVVTFDTT
                     SAVVMSIVGVLSVLMGTVSFLDSIMLIAIISFVSSVSISRFIGGGHVFNGNNKRNL"
     gene            10037..10474
                     /gene="mnhG2"
                     /locus_tag="O2701_002034"
     CDS             10037..10474
                     /gene="mnhG2"
                     /locus_tag="O2701_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit G"
                     /protein_id="HCZ4293916.1"
                     /translation="MEITKEIFSLIAAVMLLLGSFIALISAIGIVKFQDVFLRSHAAT
                     KSSTLSVLLTLIGVLIYFIVNTGFFSVRLLLSLVFINLTSPVGMHLVARAAYRNGAYM
                     YRKNDAHTHASILLSSNEQNSTEALQLRAEKREEHRKKWYQND"
     gene            10814..12856
                     /locus_tag="O2701_002035"
     CDS             10814..12856
                     /locus_tag="O2701_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCZ4293917.1"
                     /translation="MEIFETILIFIAVVILSSFVHTFIPKVPLAFIQIFLGMLLFITP
                     IPVQFNFDSELFMVTMIAPLLFVEGVNVSRVHLRKYIKPVMMMALGLVITTVIGVGLF
                     IHWIWPDLPIGAAFAIAAILCPTDAVAVQAITKGKVLPKGAMTILEGESLLNDAAGII
                     SFKIAVGVLVTGAFSLVDAVQLFLIASIGGAVVGLLIGMALVRFRLTLMRRGYENINM
                     FTIIQLLTPFVTYLIAELFHASGIIAAVVAGLVHGFERDRIMQVRTQLQMSYNHTWNI
                     LGYVLNGFVFSILGFLVPEVIIKIIKTEPHNLIFLIGITIVVALAVYLFRFVWVYVLY
                     PYFYLAISPFQKMMTKNDDDNPTTEKPPKRSLYALIMTLCGVHGTISLAIALTLPYFL
                     AGHHAFTYRNDLLFIASGMVIISLVVAQVLLPLLTKPAPKTVIGNMSFKVARIYILEQ
                     VIDYLNQKSTFETSFKYGNVIKEYHDKLAFLKTVEKDDENSKELERLQKIAFNVETKT
                     LESLVDEGQITNSVLENYMRYAERTQVYRQASLIRRMIVLLRGALLKRRVQTRVNSAS
                     SLSVTDNLMELNKINKLVHYNVVSRLSKETTKDNTLEVGMVCDGYLMRIENLTPSNFF
                     NSASEDTITKIKLNALREQRRILRELIDTDEVSEGTALKLREAINYDEMVIVDSMT"
BASE COUNT         4424 a         2016 c         2233 g         4881 t
ORIGIN      
        1 gttctgtatc tgaaattttt tcatcaggtt ttttataatc tgatggaaaa tatttcaaca
       61 attctttttc tctagattcc aattcttttg aatctttcga gatattcata tacgtatcaa
      121 taaacttatc agcatataac tttaatttag gactatactg aactgtattc gcattatctt
      181 catacttttt ttgaatcata ttcgttttat taactgcttc tttactgtca ttatttgctc
      241 ttgtagcttt aataaaagaa gctaataaaa gaattaacaa aatagctaat aatgagtaaa
      301 aacaaattac aatcatctta cgacgatttt taaattttgg aattactcgg cgttgttctt
      361 taggaatatc aaaatgcttt ccgtttttag aaaatcgtgt taaaatgtac ttgcttatta
      421 ttttcataaa cgattttcta aaatccttta cgaaattttt aataaaaatc acatcccagt
      481 tattttttta acacttatct tttgataagt gctttttttg ttttaaagcc tccaaataat
      541 gattaattct attcgtatgc tcatcattaa attgtgaatg cgctaaaatt ttatctaaaa
      601 aatcagtatc tgtaagcaac ccctcagatt gcgccatact ttcggcagtt aacactgtat
      661 ctaaagtagt agcaacttgt ttacataacc aatcaagatt atcctcaatg gactttaatg
      721 ttggtctagt agttaaattc aatttttcag tatctttaat aaataacgcc caaggtctat
      781 ataatggcca ccgcttacga tttttatcat ttccaccttt aggaggagag ataaaacgca
      841 aattattatg caatatagac tttacaattt cgcttatatt atctgttcta ctcaacactt
      901 tcgcacaatt aactgcatat gcctttcgca tttgtatttc ataacgattc catagcccaa
      961 tgtcttcgac atcacaatga cgtttaaacg cttgttcata attcttttca taaaaacgac
     1021 aatataaatt gctttgttta gaaccaatat aaaaagtagc gcctttactt cttccgtctg
     1081 acaaattaaa accgtttatt gcgcttccag ctctaaaacg tgaaacacac tcagcttttt
     1141 cagccttttt aattaataaa ggcactttta aatatgtttt ataatcatca attgcaatat
     1201 caattcttgt aaaatttgca ttaactgata aacatttgtc aaaaaaatct tgccaagttt
     1261 tattcaactg ctgtaaatga tgttcaaact ctctacaacc tgtaccagtt aactgtaagt
     1321 gaatgcccat tgattcatca tcacttgaat aatacaattt aatgtttgaa aattcataac
     1381 cgcgattata aaagtaaatg ccactatttc gttttttgaa caacgttata ggcaaaccga
     1441 taacgtcctc aattactgct gaaatagggg ctatatggaa agtgacctga acccagtcaa
     1501 caactgcctc cacagcactt ttatttgtac gctctacccc cctattagta aggggggtac
     1561 actggttttt taaagtcata tttatttatg cctcctatca aatcgcaagc gatttgatac
     1621 atgaccgttc tcgctttttt aacgcgtatt tgtcgcaggc tccgcccaaa tgcttaacgc
     1681 actccgtgct tgcatttctg cgcctgcata cgctaaaagc tacacggtca tgttaaatca
     1741 ctaatactat atgttaaaca tcaatatcgc caatataaat acaatcttcc aaattcactt
     1801 gtagcattct gccatgtcct gcttcactca agctcataca caatagacaa aggtcagcgt
     1861 aaattttagg ccgacctttt accgtatttt ctttaacgta actatcatta aatttagact
     1921 gtatttcttt agctaaagtc attaatgaaa ctttatcgcc ccaatttata cgactagcaa
     1981 catagtttaa aataggttca ctcaaatttg aactatttgt gtagcgatat aatctatctg
     2041 ctgtataata atccatttac atcacctaat tatttttgtt taagtgcact attagaacca
     2101 actactttaa tatcttctgc attccagcta tatgcgatag ttccaaaatc gcctgacatt
     2161 tgaacataca ttttaacgtc tggttcaact aattcgcatt cttgtaaata gtctaattcc
     2221 actttattag gcgttttaac agtgatttgt tcgccttgtt cttgactcgc taatttatat
     2281 gcataaacag ttttatcagt acgctctcca ttttcatatt tatatttttc atctacacct
     2341 aaaaagttta attgtcctaa agttttttta atatccaata ctggtttcat aatatatcca
     2401 tcctttacaa aatttttaat aaaattaaac gacttctaaa gggtcgcacg aataggaata
     2461 actcccgcaa atccgtacca ctgcattttt gataatggag tgtctatgct tctcctgacc
     2521 tatcatattt ttaatcaaac ctatcggttc atcacctcaa aaaattctaa gtcttcaatt
     2581 gaaatatgat acatcttaca aataaatgta ttagacacac catattgttt taaaaattta
     2641 taatccttat aagtaaatcg ataatcattc ttttttgcca tttcacaaac ttctaatata
     2701 cgaaaatatg caaaattata cattttacaa atttttcttt tagaaacacc atgaaatctt
     2761 aaaattacaa atttaagata atttaattga tttgaaccat caaaaaaagg tgtaaactca
     2821 gtcataatta tcaattcctt tcataagaat taactgttaa ctaaagttta caccaccaat
     2881 taaaatcatg aattgtagcg ccaaataatc caaatgtatc aataatgaca cccataataa
     2941 tttgacccgc aactgttgca attacagtta atgttgcacc taattttggc aataacaata
     3001 aattgccagt taaaaagcta accccaagca aaccaccgac tacccatgtg tagttaaatg
     3061 attgattatt gtaaaagtga atagtaaata cttctggatt gataatgata tttaaaataa
     3121 ttaaacaaat tgttccaact gaaaatgaaa tgaatgaagt atagaaaggc gatttagtat
     3181 acagtgatag tcttgaattg acagatgttt ggataggaat aagcattcca actaggacac
     3241 caatgatata gaaaagaagc atatgaataa agtctcactt tccaaagtta ataataaaat
     3301 catatattta cattatcatt atagagaaaa tatacaactc atttacaata atgttgaaaa
     3361 aaacaggaca ttattacaac aaaatcaccc tagacaatca tgatgaaagt ctagggtgtt
     3421 agtatttata ctaaattaat tgcgctttct ttatatctat ttcttatctt caaataaaac
     3481 agttgttgtt ttatcaataa tttgagcaga atgtactttg gtgatagtac cactatttga
     3541 ctttataata tttaaatcta ctaatgcatt catactatca cgtgcaactt gctcggtcac
     3601 tacgttgttt aattgtggta attgcagttt tacaggcttg tctagtgaag atttaaaact
     3661 taattcaagt gttttagtca tgaatatgtc ctcctgatta aattgataaa gatttgatga
     3721 gttcgatatt gctatatgtt tctccagtaa gtcgctcaat aagtttgctg aatgttttaa
     3781 tttggtcgtt tgatgcatca gggttaatgt tagcgaatcg acgcttaaat tctgtttgtt
     3841 tgccgttagc gtctacttta gtaaatgata atacaatagt gatgtggttt attttactca
     3901 tattttaaaa cctcctttca cactatatat cgaaacaaaa taataaaatg gctaatttta
     3961 ttttctatgt ttaaaatcta taaaaaaggc aatagatata tgtaactaaa atataatata
     4021 gctatgatta agacacttaa agcaagggga ggttgagtaa tgaataaagt agaagcgatt
     4081 aaatttaatg atgatattgt taaaatgtat gaagcgctca agataaaatc tgaacgtgac
     4141 tatttattct ttaagttagc tatacatagt ggattgaaag tatcagaatt attaacaatt
     4201 acagtctctc aagttaagag actaattgaa aagtgtacgt tatcagaaat gtgtaaagca
     4261 cattttcatt cgttgattaa aattaggtta ccagaaacat tatcgaaaga actacttcaa
     4321 tatatagagg acaggagtct ttcgaatgaa gacgttcttt ttcaatcact acgaacaaat
     4381 caagtattat ctagacagca agcatatcga ataattcacc aagcatcaat tgaagctggt
     4441 atagataatg taggactaac gacattgcgt aagacatttg catatcatgc ttatcaaaaa
     4501 ggtataccta taccagtcat tcaaaagtat ttagggcatc aatctgctat tgaaacacta
     4561 aattttatcg gtttagaaaa tgagtgtgaa catagtattt atatttcatt acaattatag
     4621 aaacaaagga ggctaataat gagtttggtt tatttattaa ttgctatact tgtgattatg
     4681 gcgatgatac ttctaatgtc taaacgtaga gcattggcta aatatgccgg gtacatagcg
     4741 ttggttgcac ctgtaatttc atctatctat tttttgattc aaataccatc agtagctaaa
     4801 ctgcaatatc tttctacctc tattccatgg attaagacat tagatattaa tttagattta
     4861 cgtttagatg gtttaagttt aatgttttct cttattattt cacttattgg aattgcagta
     4921 ttcttctatg caactcaata tttatcctct cgaaaagaca atttaccaag gttttatttt
     4981 tatttaacgt tatttatgtt cagtatgatt ggtattgtat tatcagacaa tacgatattg
     5041 atgtacattt tttgggaatt aacgagtgta tcatcatttt tattgatttc atattggtat
     5101 aacaatggtg acagtcaatt tggtgcgatt caatcattta tgattacagt atttggtgga
     5161 ttggcgttat tagttggttt tattatgctg tatatcatga caggaacgaa taacatcaca
     5221 gagatattag gacaagcaga tcatattaag aatcatggat tgtttatccc tatgattttt
     5281 atgtttttat taggtgcatt tacaaaatca gcacaatttc catttcatat ttggctacct
     5341 agagcaatgg ctgcacctac acctgtaagt gcttatttac attcagccac gatggtaaaa
     5401 gctggtatct ttttattact tcgatttaca ccattattag gtcttagcaa tatgtacgta
     5461 tatatcgtta cgtttgttgg tttaataaca atgttatttg gttcaattac agctttaaaa
     5521 caatgggatt taaaaggtat cctagcgtac tctacaatca gtcaacttgg gatgattatg
     5581 gctatggtgg gtataggtgg cggatatgct caacaccaac aagacgcaat agcatctatt
     5641 tatgtatttg tattatttgg tgcgctattt catctaatga atcatgccat ctttaaatgt
     5701 gcgcttttca tgggagtagg tattttagat catgaagcag gttcaaggga tatacgaatt
     5761 ttaagtggaa tgcgtcaact atttcctaaa atgaatctag tcatgacgat agcggctcta
     5821 tctatggctg gagtaccatt tttaaatgga tttttaagta aagaaatgtt tttagatgca
     5881 ttaacacaaa ctggacaatt atcccaattt agtttgattt caatgatagc tatcgtgttt
     5941 gttggtgtta ttgcgagtgt ttttacattc acatatgcac tatacatggt aaaagaagta
     6001 ttttggacaa aatatgattc taaggttttt actaaaaaaa atatccacga accatggttg
     6061 tttagtttac catctcttat attaatggtg ctagtacctg taatcttttt tgtaccaaat
     6121 atatttggga aggggattat cgttctagca ttaagagctg tatcaggtgg taatcatcaa
     6181 attgatcaat tggcaccaca tgtttcgcaa tggcatggat ttaacatacc gcttctttta
     6241 accatcatca ttattttatt gggtagtgta ctagcaatca aagtagattg gaaaaaagtg
     6301 ttcacaggta aaattagaca gatttcagtt tcaaaaagct atgagatggt atatcgacat
     6361 tttgaaaagt ttgctacgaa gcgatttaaa cgtgttatgc aagatcgttt aaaccaatac
     6421 attattatga ccttaggcat atttatgatt atcattggat atggttatat tcgaattgga
     6481 cttcctaaag tacatcagtt acatgtttct gaatttgggg cattagaaat tatattagca
     6541 atcgtaactg tcacaattgg tatttcttta atttttatac gtcaacgact gacaatggtc
     6601 attttaaatg gagtcatcgg atttgttgtg accttattct ttatagcaat gaaagcccct
     6661 gatctagcat tgactcagct agtagttgaa acaataacga cgatactatt tattgtcagt
     6721 ttttcaagat taccaaacgt gccaagatct aacgctaaca aaaaaagaga aataattaaa
     6781 atttctgtat cactcttgat ggcacttatt gttgtatcat taatttttat tacacaacaa
     6841 acagatggtt tatcatcaat atcagacttt tatttaaaag ctgacaaact aacaggtggt
     6901 aaaaatattg taaatgcgat acttggtgac tttagagcat tagatacatt atttgaagga
     6961 ttagtgttaa ttattactgg gctaggtatt tacacattat taaattatca agatcggagg
     7021 ggacaagatg aaagagaatg atgtcgtgtt aagaacggtc acgaaacttg ttgtatttat
     7081 tttattgact ttcggattct atgtcttctt cgcaggtcat aataatcctg gtggtgggtt
     7141 tattggtggt ttaatattta gttcagcgtt tattttaatc tttctggctt ttaatgttga
     7201 agaggtttta gaaagtttac cgattgattt tagaatttta atgattattg gagcattggt
     7261 atcatctatt actgcgataa tacctatgtt ttttggaaaa ccatttttgt ctcaatatga
     7321 aacaacttgg atacttccaa ttttaggaca aattcatgta agtacaataa cactttttga
     7381 attaggtatt ttattctcag ttgttggtgt tattgtcaca gtgatgttgt cgcttagcgg
     7441 aggtcgatca tgaatttaat attattacta gttataggat ttttagtgtt tataggaaca
     7501 tatatgattt tatcaatcaa tttaattcgt attgtaatcg gaatttcaat atatactcat
     7561 gctggtaatc tcattattat gagtatggga acgtatggtt ctagtagatc agaaccacta
     7621 ataactggtg gaaaccaatt gtttgttgat cccttgttac aagctattgt actaactgca
     7681 atagttatag ggtttgggat gactgcgttt ttacttgtac ttgtttatag aacttataaa
     7741 gtaacaaaag aagatgaaat tgaaggccta aggggggaag atgatgctaa gtaacttatt
     7801 gattttacca atgttattac cattcctttg tgccttaatc cttgtatttt taaaaaataa
     7861 tgatcgtatt tctaaatatt tatacttagg tacaatgact atcaccacaa ttatttcatt
     7921 aatgctatta atttatgttc agcgtcaccg tccaattacg ctagactttg gaggatggtc
     7981 agcgcccttt ggtatacagt ttttaggaga ttctttaagt ttaattatgg ttacaaccgc
     8041 ttcgtttgtg attactttaa ttatggcata cggatttggg cgtggcgaac ataaagcaaa
     8101 tcgttatcac ttgccatcgt tcatattatt tttaagtgtt ggcgtgatag gctcttttct
     8161 aacatcagat ttatttaatt tatacgtcat gtttgaaatt atgttactag cgtcatttgt
     8221 actcattaca cttggacaat ctgtagaaca attacgtgct gcaattattt atgttgtctt
     8281 gaatattatt ggttcatggc tattcttatt aggtataggt ttactttata aaacagtagg
     8341 tacattaaac ttttcacata ttgcaatgcg tttgaatgac atgggagata atcgcactgt
     8401 tacaatgatt tcattaatct tcttagtcgc atttagtgcg aaagcagcgc tggtcctttt
     8461 tatgtggcta cccaaagcct acgctgtgtt aaatactgag cttgcagcat tatttgcagc
     8521 gttaatgacc aaagtagggg cctatgcatt aattcgattc ttcactttac tatttgatca
     8581 acataatgat ctcatacatc cattgctagc aactatggct gctataacta tggtcatcgg
     8641 cgctataggt gtcattgctt ataaagatat taaaaagatt gcagcttacc aagtcataat
     8701 ctcaatagga tttatcattt taggtttagg aacaaacacg tttgcaggta ttaatggtgc
     8761 aatattttat ttggtaaatg acattgttgt aaaaacattg ctatttttta ttattggtag
     8821 tttagtttac attacaggct atcgacaata tcaatatttg aatggcttag ctaaaaaaga
     8881 acctttattt ggagttgcgt ttattataat gatttttgct attggcggcg tgcctccatt
     8941 tagtggcttt ccggggaaag tacttatttt ccaaggtgca ttgcaaaatg gcaattatat
     9001 tggactagcg ttaatgatta ttactagtct aattgcaatg tacagtttat ttaggatact
     9061 tttttatatg tattttggag ataaagatgg ggaggaagtt aattttaaga aaatcccgct
     9121 atatcgaaaa agaattttaa gtattttagt agttgtggtt atcgcaatcg gaattgctgc
     9181 acctgttgtg ttaaatgtta caagtgatgc aactgagttg aacacgagtg atcaattata
     9241 tcaaaaactt gtaaatccgc atttgaaagg agaggactaa atgaatcaaa tagttttaaa
     9301 tattatcatt gcattcttat gggtattatt tcaagatgaa gatcatttta aattctcgac
     9361 tttcttttct ggatatctaa ttggtttaat tgtcatttat atattacaca ggtttttcag
     9421 cgatgatttt tatgttagaa aaatatgggt agctattaaa tttttaggtg tttatttata
     9481 tcaattaata acatctagca ttagcacgat taattatatt ctttttaaaa caaaagatat
     9541 gaaccctgga ttactttcat atgaaacaag actaacaagt gattggtcaa taacattttt
     9601 aacaatttta attattataa ctccagggtc tacagtaata cgaatttctc aagactctaa
     9661 aaagtttttt attcatagta tcgacgtgtc agaaaaagaa aaagatagtt tgttaagaag
     9721 tattaagcat tatgaagact taatattgga ggtgtcgcga tgatacaaac aataacacat
     9781 attatgatta ttagttcact cattattttt ggaattgcat taatcatctg tttatttaga
     9841 ttaatcaagg gacctacaac agcagatcgt gtcgttacat ttgatacaac aagtgctgtc
     9901 gtaatgtcaa ttgtgggtgt gttaagtgta cttatgggca ccgtttcttt cttagattca
     9961 atcatgctca ttgccattat atcttttgta agttctgttt caatatcacg ctttattggt
    10021 ggggggcatg tgtttaatgg aaataacaaa agaaatcttt agtcttattg ctgctgtgat
    10081 gttgttgtta ggtagtttta ttgctcttat tagtgcaata ggtatcgtga aattccaaga
    10141 tgttttctta agaagtcacg ctgcgacaaa aagttcaact ttatccgtgt tattaacttt
    10201 aatcggtgta ttaatttatt ttattgtgaa tacaggattt ttcagtgtgc gtttattact
    10261 gtcacttgtt tttattaatt taacttcacc agtcggcatg cacttagtcg ctcgcgctgc
    10321 ttatcgcaac ggcgcttata tgtatcgaaa aaatgatgct cacacacatg catcaatatt
    10381 attaagttca aatgaacaaa actctacaga agcattacaa ttacgtgctg aaaaacgaga
    10441 agagcatcgt aagaaatggt atcaaaacga ttgaagtgtt ggctaaattg ttaagcataa
    10501 caatgctttt gaaaatctgt tttcaaaatt aatcattaaa agagtacttt gaaattggtt
    10561 agtcgatttt aaggtgctca ttttttgtat tataagttaa tgttgttatg tgaaagcaag
    10621 ttgtttattt atacaaacaa ttagtgataa aataaaatta aataattgca aatttaagta
    10681 ctgcaatcgt agacatatat ctatattaaa gtgcttttgt ggtcgctaac tcaatttatc
    10741 ctctaaagta taattacaac gagatgtgat acttaatttt caattaaaca atacttttta
    10801 gagaggtgaa agtttggaaa tatttgaaac aattcttata tttatagctg ttgtgatact
    10861 aagttcgttt gtccatactt tcatacctaa agtaccccta gcatttatac aaattttctt
    10921 gggcatgtta ctatttatta ccccaatccc tgttcaattt aattttgatt ctgaattgtt
    10981 tatggtaaca atgattgcgc ctttgttatt tgtagaaggt gttaatgttt ctagagtcca
    11041 tttaaggaaa tatattaagc cagtgatgat gatggcatta ggattagtca ttactactgt
    11101 gataggtgta ggtttattta ttcattggat ttggccagat ttacctattg gagcagcatt
    11161 tgcaattgct gccattcttt gtcctactga tgcagtagca gtgcaagcaa tcactaaagg
    11221 aaaggtcttg ccaaaaggag caatgacaat tcttgaaggt gagtcattat tgaatgatgc
    11281 tgctggtatt atttcattta aaatagctgt tggagtatta gttacaggtg ctttttcact
    11341 tgttgatgct gttcagttgt ttttaattgc atcaattggt ggcgcagtgg ttggtttact
    11401 tataggtatg gcattagtaa ggttccgatt aacattgatg cgtcgaggat atgaaaacat
    11461 taatatgttt acaattattc aattgttaac accatttgtt acgtatttaa ttgctgaatt
    11521 gtttcacgca tcaggaatca ttgcagcagt agttgcagga cttgtacatg gtttcgaacg
    11581 tgacagaatt atgcaagtac gtacacaact gcaaatgagt tacaatcata catggaatat
    11641 actaggttat gttttaaatg gctttgtttt ttcaatatta ggatttttag tacctgaagt
    11701 tattattaaa attatcaaaa cagaaccgca caatttaatc tttttaatag gcatcactat
    11761 tgttgttgct ttagctgtct atctatttag atttgtttgg gtttatgtct tatatcctta
    11821 tttttattta gccatcagtc cattccaaaa aatgatgact aaaaatgatg atgataatcc
    11881 aacgactgag aaaccaccaa agcgaagttt atacgcttta attatgacgt tatgtggtgt
    11941 gcatggaaca atttctttag caatcgcatt aacgttaccg tattttttag cagggcatca
    12001 tgcttttacg tatagaaacg acttattatt tattgcatct ggtatggtta ttattagttt
    12061 ggtagttgcg caagtattat tgccattatt aacgaaacct gcacctaaaa cagtaattgg
    12121 caatatgtcg tttaaagttg ctagaattta tatattagaa caagttattg attatctaaa
    12181 tcaaaaatct actttcgaaa caagttttaa atatggtaac gtgattaaag aatatcatga
    12241 taaattagca tttttaaaaa ctgtagagaa agatgatgaa aactctaaag aattagaacg
    12301 tctacaaaaa attgctttta atgtagaaac aaaaacatta gagtctttag tagatgaagg
    12361 acaaataacg aatagtgtac ttgaaaacta tatgcgttat gctgaaagaa cacaggtata
    12421 tagacaagca tcattaataa gaagaatgat tgtattatta cgaggtgctt tattaaaacg
    12481 aagagtacaa acgagagtga actccgcatc ttcacttagt gttacggata acttaatgga
    12541 attaaataaa attaataaat tagtccatta taatgtggtt agtcgtttgt ctaaggaaac
    12601 aacaaaagat aatacacttg aagttggaat ggtttgtgac ggttatttaa tgcgaattga
    12661 aaacttaaca ccatcaaatt tcttcaactc agcaagtgaa gatacgatta ctaaaattaa
    12721 attaaatgca ttgagagaac aacgtcgcat tttacgtgag ttgattgata cagatgaagt
    12781 atcagaaggt acagcgttaa aactaagaga agccatcaat tatgatgaaa tggttattgt
    12841 agatagtatg acgtagttcc taattatgct aaaagggatt gatgaaaaac tgaagggctt
    12901 ttcatcaatc ccttttattt taggggaatt gaatagatag ttttaaacta tacgaattat
    12961 taatatttga gatttaattg aaataagttt taaaaattgg aggagataga ttaagcgaag
    13021 tcatttaaag gtgaagttaa gtgtattcac aaaaaatagc cacactcata tgacatcgga
    13081 tgagtgtggc ttaaggatct atggggggag gaaaccatag atgtttactt tgataggcca
    13141 gattaaatat caaagtatgc gattatttat agcttgatgc aaaagtggta tgcctattaa
    13201 aagttactgc acatagcttt taatattccg ttcaaaggaa aggggcatac aattgaacaa
    13261 tctgtaatag tacttttaac cagctatgct aaaagtctag tagggagaac agttgtccaa
    13321 tcacataaga acctctaact tcgttagtac gattaagaaa agctttttag ttagtatgta
    13381 atacaattta ttgacgcgcg tgaatctctt ttataagagt gtgtagggaa tggcgttgta
    13441 taaattgtat tagaagaact tctaacgcat ctctgtggtt aaaagagatg aagggaacga
    13501 cagtttaatt aaaactgcat aagaacttct agcttttctc tctcgttcaa agag
//
LOCUS       DALAGD010000080        13365 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.081, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000080 DALAGD010000000
VERSION     DALAGD010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13365
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.081"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            2..874
                     /locus_tag="O2701_002036"
     CDS             2..874
                     /locus_tag="O2701_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4293918.1"
                     /translation="MITITFFLMKLMPGSPFNDAKLNAEQKEILNEKYGLNDPVATQY
                     LHYLKNVVTGDFGNSFQYHNQPVWDLIKPRLLPSFEMGLTAMFIGVILGLILGVAAAT
                     KQNSWVDYTTTVISVIAVSVPSFVLAVLLQYVFAVKLRWFPVAGWEGFSTAVLPSLAL
                     SAAVLATVARYIRAEMIEVLSSDYILLARAKGNSTMRVLFGHALRNALIPIITIIVPM
                     LASILTGTLTIENIFGVPGLGDQFVRSITTNDFSVIMAITLLFSTLFIVSIFIVDILY
                     GVIDPRIRVQGGKK"
     gene            874..1944
                     /locus_tag="O2701_002037"
     CDS             874..1944
                     /locus_tag="O2701_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4293919.1"
                     /translation="MAENKNNLSINDDHSNAAMTHTSDAIASSDFIIRELDLNQEPEM
                     QRESKNFWQDAWAQLKRNKLAVVGMIGLIIIVIFAFIGPVINKHDYAEQNVEHRNLPA
                     KIPVLDKVPFLPFDGKDADGKDAYKAANAKENYWFGTDQLGRDLWTRTWKGAQISLFI
                     GVVAAMLDIFIGVVYGAISGFFGGRVDTIMQRILEVIASIPNLIVVILFVLIFEPSIW
                     TIILAMSITGWLGMSRVVRGEFLKLKNQEFVMASKTLGASKFKLIFKHILPNTLGAIV
                     VTSMFTVPSAIFFEAFLSFIGIGVPAPQTSLGSLVNDGRAMLLIYPHELFIPAMILSL
                     LILFFYLFSDGLRDAFDPKMRK"
     gene            1961..3043
                     /locus_tag="O2701_002038"
     CDS             1961..3043
                     /locus_tag="O2701_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4293920.1"
                     /translation="MTERILEVNDLHVSFDITAGEVQAVRGVDFYLNKGETLAIVGES
                     GSGKSVTTKAITKLFQGDTGRIKKGEILFLGEDLAKKPENELIKLRGKDISMIFQDPM
                     TSLNPTMQIGKQVMEPLIKHKNYSKAQAKKRALEILNLVGLPNAEKRFKAYPHQFSGG
                     QRQRIVIATALACEPKVLIADEPTTALDVTMQAQILDLMKELQQKIDTAIIFITHDLG
                     VVANIADRVAVMYGGQMVETGDVNEIFYDPKHPYTWGLLSSMPDLSTTNDTPLLAIPG
                     APPDLLHPPKGDAFARRSQYALDIDFKVEPPWFKVSPTHFVKSWLLDARAPKVELPEL
                     VKQRMKPMPNNYEKPLKVERVSFNEK"
     gene            3033..3974
                     /locus_tag="O2701_002039"
     CDS             3033..3974
                     /locus_tag="O2701_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCZ4293921.1"
                     /translation="MKNDEVLLSIKNLKQYFNAGKKNEVRAIENISFDIYKGETLGLV
                     GESGCGKSTTGKSIIKLNDITSGEILYEGIDIQKIRKRKDLLKFNKKIQMIFQDPYAS
                     LNPRLKVMDIVAEGIDIHHLATDKRDRKKRVYDLLETVGLSKEHANRYPHEFSGGQRQ
                     RIGIARALAVEPEFIIADEPISALDVSIQAQVVNLLLKLQRERGITFLFIAHDLSMVK
                     YISDRIAVMHFGKIVEIGPAEEIYQNPLHDYTKSLLSAIPQPDPESERSRKRFSYIDD
                     EANNHLRQLHEIRPNHFVFSTEEEAAQLRENKLVTQN"
     gene            3993..5648
                     /locus_tag="O2701_002040"
     CDS             3993..5648
                     /locus_tag="O2701_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCZ4293922.1"
                     /translation="MTRKFRTLILILIATIALSGCANDDGIYSDKGQVFRKILSSDLT
                     SLDTSLITDEISSEVTAQTFEGLYTLGKGDKPVLGVAKAFPEKSKDGKTLKVKLRSDA
                     KWSNGDKVTAQDFVYAWRKTVDPKTGSEFAYIMGDIKNASDISTGKKPVEQLGIKALN
                     DETLQIELEKPVPYINQLLALNTFAPQNEKVAKKYGKNYGTAADRAVYNGPFKVDDWK
                     QEDKTLLSKNQYYWDKKNVKLDKVNYKVIKDLQAGASLYDTESVDDAVITADQVNKYK
                     DNKGLNFVLTTGTFFVKMNEKQYPDFKNKNLRLAIAQAIDKKGYVDSVKNNGSIPSDT
                     LTAKGIAKAPNGKDYASTMNSPLKYNPKEARAHWDKAKKELGKNEVTFSMNTEDTPDA
                     KISAEYIKSQVEKNLPGVTLKIKQLPFKQRVSLELSNNFEASLSGWSADYPDPMAYLE
                     TMTTGSAQNNTDWGNKEYDQLLKVARTKLALQPNERYENLKKAEEMFLGDAPVAPIYQ
                     KGVAHLTNPQVKGLIYHKFGPNNSLKHVYIDKSIDKETGKKKK"
     gene            5860..7575
                     /locus_tag="O2701_002041"
     CDS             5860..7575
                     /locus_tag="O2701_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCZ4293923.1"
                     /translation="MGKLIKYISILLIVVLVLSACGKSSNKDEGVKDATKTETSKHKG
                     GTLNVALTAPPSGVYSSLLNSTHADSVVEGYFNESLLATDKKIRPKAYIASWKDIEPA
                     KKIEFKIKKGIKWHDGNELKIDDWIYSIEVLANKDYEGAYYPSVENIQGAKDYHEGKT
                     DHISGLKKIDDYTMQVTFDKKQENYLTGFITGPLLSKKYLSDVPIKDLAKSDKIRKYP
                     IGIGPYKVKKIVPGEAVQLVKFDDYWQGKPALDKINLKVIDQAQIIKAMEKGDIDVAN
                     DATGAMAKDAKSSNAGLKVLSAPSLDYGLIGFVSHDYDKKANKTGKVRPKYEDKELRK
                     AMLYAIDREKWIKAFFNGYASEINSFVPSMHWIAANPKDLNDYKYDPEKAKKILDKLG
                     YKDRDGDGFREDPKGNKFEINFKHYSGSNPTFEPRTAAIKDFWEKVGLKTNVKLVEFG
                     KYNEDLANASKDMEVYFRSWAGGTDPDPSDLYHTDRPQNEMRTVLPKSDQYLDDALDF
                     EKVGIDEKKRKDIYVKWQKYMNDELPGLPMFQGKSITIVNDKVRNLDIEIGTDQSLYN
                     LTKEA"
     gene            7625..8611
                     /locus_tag="O2701_002042"
     CDS             7625..8611
                     /locus_tag="O2701_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4293924.1"
                     /translation="MNNVLLEVKDLETSLKINNEWLATVENISFELSKGEVLGIVGES
                     GCGKSILSKSIIKLLPEKISKLSNGEVIFDGKRIDTLNEKQLPDIRGNDIAMIFQEPM
                     TALNPVFTIKNQLVESIKSHKKISKKEANKLAKDLLKKVGIARQDEILNSYPHQLSGG
                     MRQRVMIAMAISCSPKLLIADEPTTALDVTIQAQILDLLKELQKETQMAIMMITHDLS
                     VVAEFCDKVLVMYAGQIVEFGGIKEILHNPKHPYTQKLLSTIPKLKEEQKRLETIEGI
                     VPSIQAFHVNKCRFANRCNKKLDICNNQSPKMHVCEDVIVRCHLYKNEYKEI"
     gene            8614..9594
                     /locus_tag="O2701_002043"
     CDS             8614..9594
                     /locus_tag="O2701_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCZ4293925.1"
                     /translation="MENILEVNQIKKYYKIKTGLLQKTQYVKAVDDVSFSIKKGQTFG
                     LVGESGCGKSTLGKVIIRLEDATSGSIIVNGEDITRLQGKKLRKSRQQYQMIFQDPYA
                     SLNPMQMVGDIISEPILNYKKLPKEEIKKEVLYLLKCVGLSEDAYYKYAHEFSGGQRQ
                     RVGIARALALRPSLIVADEPVSALDVSVQSQVLNLLKDLQEQFNLSYLFIAHDLSVVK
                     HISDVIGVMYLGHIVEIASDKEIYENPKHPYTKALISSIPQIDKHNNNRIILKGELPS
                     PSNPPQGCPFHTRCPIAQDMCKKSMPELKDIGNEHQVACFYVDKVGDLND"
     gene            9587..10549
                     /locus_tag="O2701_002044"
     CDS             9587..10549
                     /locus_tag="O2701_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4293926.1"
                     /translation="MIKLILKRLGLMIPLLILISIVVFSLAIIQPGDPFSDLQNGKIK
                     QEAINAQREKLGLNDSIPHQYIRWVNHVIHGDLGESIKYKRPVIDVIEERIPNTILLG
                     AMSLIITYIISFALGITSGRYSYSLTDYTVQIFNYLMLAIPSFIAGVFAIFIFSFELQ
                     WFPFQGSVDINLKEGTFEYYMSKIYHTFLPAFTLGLLSTAGYIQYLRNDIIENSKKDY
                     VLTARSKGLSMNKIYNKHILRNSLIPIITFLGADIVSILGGAVITETIFSYNGIGKLF
                     LESVIGQDYPLMMALTLFFSFLGLLGNLISDITYGFIDPRIRSN"
     gene            10560..11441
                     /locus_tag="O2701_002045"
     CDS             10560..11441
                     /locus_tag="O2701_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4293927.1"
                     /translation="MQNKSKSPFKIAFSKFIHNKIAMLSVIFLLIITIVSIIAPLIAP
                     FPVNQQDLLNIKGEMTAQNILGTDSGGRDNFSRLLYAGRISLSIGITSTIGMLLIGIT
                     VGVISGYFGGIVDTLLMRITEFVMLFPFLIFAIVLNAALGDKIKNPYGSAIILVLVII
                     VLSWGGIARLVRGKVLQEKENEYFLAAKSIGTPTYKIILKHLLPNILSVVIVQATLLF
                     AGMIVVESGLSFLGFGISKAIPSWGNMLSDAQEGDVISGKPWIWMPPAIMITLTILSI
                     NFVGEGIKDAFNPRGRR"
     gene            complement(11483..12472)
                     /gene="trpS"
                     /locus_tag="O2701_002046"
     CDS             complement(11483..12472)
                     /gene="trpS"
                     /locus_tag="O2701_002046"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCZ4293928.1"
                     /translation="METLFSGIQPSGIPTIGNYIGALKQFVDVQNDYDCYFCIVDQHA
                     ITMPQDRLKLRKQTRQLAAIYLASGIDPDKATLFIQSEVPAHVQAGWMLTTIASVGEL
                     ERMTQYKDKAQKAVEGIPAGLLTYPPLMAADIVLYNTNIVPVGDDQKQHIELTRNLVD
                     RFNSRYNDVLVKPEIRMPKVGGRVMSLQDPTRKMSKSDDNAKNFISLLDEPNVAAKKI
                     KSAVTDSDGIIKFDRDNKPGITNLISIYAGLTDMPIKDIEAKYEGEGYGKFKGDLAEI
                     VKAFLVEFQEKYESFYNSDKLDDILDQGRDKAHKVSFKTVKKMEKAMGLGRKR"
     gene            12767..13162
                     /gene="spxA"
                     /locus_tag="O2701_002047"
     CDS             12767..13162
                     /gene="spxA"
                     /locus_tag="O2701_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCZ4293929.1"
                     /translation="MVTLFTSPSCTSCRKAKAWLQEHDIPYTERNIFSEHLTIDEIKQ
                     ILKMTEDGTDEIISTRSKTYQKLNVDIDSLPLQDLYSIIQDNPGLLRRPIILDNKRLQ
                     VGYNEDEIRRFLPRKVRTFQLQEAQRMVD"
BASE COUNT         4828 a         1876 c         2416 g         4245 t
ORIGIN      
        1 tattattaca attacatttt tcttaatgaa attaatgcca ggttcgccat ttaacgatgc
       61 taaattaaat gctgaacaaa aagaaatttt aaatgaaaaa tatggattaa atgatcctgt
      121 agctacgcag tatttacatt atttaaaaaa tgttgttaca ggcgattttg gtaattcatt
      181 ccagtatcat aatcaacctg tgtgggattt gattaaaccg agactactac cttcttttga
      241 aatgggtctt acagcaatgt tcatcggtgt gatactggga cttattttag gtgttgcagc
      301 agctactaaa caaaattctt gggttgacta tacaactaca gttatttcag ttattgcagt
      361 atctgtacca tcttttgtac ttgctgtact tttacaatat gtatttgcag ttaaattaag
      421 atggttccca gtagctggat gggaaggttt ttcgaccgcg gtattaccgt cacttgcatt
      481 atctgcagct gttttagcaa ctgtcgccag atacataaga gcagagatga tagaggtatt
      541 aagttcagac tatattttat tagcgagagc taaaggtaat tcgacaatgc gtgtactttt
      601 tggacatgca cttagaaatg ctttaattcc aattattaca attatcgttc ccatgttagc
      661 aagtatttta acaggcactt taacaattga aaatattttt ggagttcctg gattagggga
      721 tcaattcgta cgttcaatta caacaaatga tttctcagta atcatggcaa tcacactatt
      781 atttagcaca ctgtttatcg tttctatttt tattgtagat attttgtacg gtgtgataga
      841 tccacgaatt cgtgttcaag gaggtaaaaa ataatggctg aaaataaaaa caatttgtcg
      901 attaacgacg atcattctaa tgcagctatg acgcatacct ctgacgctat cgcatcatct
      961 gattttatta ttagagaatt agatttgaat caggaacctg aaatgcaacg agaaagcaaa
     1021 aacttttggc aagatgcttg ggctcagtta aaacgaaata agttagctgt tgtcggtatg
     1081 ataggtttaa ttatcattgt aatatttgct tttatcggtc cagttataaa taaacatgat
     1141 tatgctgaac aaaatgtaga acatagaaat cttccggcaa aaatacctgt attagacaaa
     1201 gttccatttt taccttttga tggtaaagat gcagatggca aggatgctta taaagcagca
     1261 aatgctaaag aaaattattg gtttggtact gatcagttgg gtcgagattt atggacaaga
     1321 acatggaaag gtgctcaaat ttcattgttt atcggtgttg ttgcagcgat gttagatatt
     1381 tttattggtg ttgtatatgg tgcgatttct ggattcttcg gtggacgtgt cgatacgatt
     1441 atgcaacgta tacttgaagt catagcatct attccgaatt taattgtcgt aattttattt
     1501 gtattaattt ttgaaccatc catttggaca attatattgg ctatgtctat cacaggctgg
     1561 ttaggcatga gcagagttgt acgtggagaa tttttaaaat taaaaaatca agagtttgtc
     1621 atggcttcga aaacattggg ggcttcaaaa ttcaaattga tatttaagca tattttacct
     1681 aatacattag gtgctatcgt ggttacatca atgtttacag tacctagtgc tattttcttc
     1741 gaagcatttt taagtttcat tggtataggt gtacccgcac ctcaaacatc gttagggtca
     1801 ttagtaaatg atgggcgcgc aatgttatta atttatccac atgaattatt tataccagca
     1861 atgattttaa gtttattaat tctattcttt tacttattta gtgatggatt acgtgatgca
     1921 tttgatccga aaatgcgtaa ataaaaaggg ggcatagcat atgactgaaa gaatattaga
     1981 agtaaatgat ttgcatgttt cctttgatat tacagcaggg gaagtgcagg cagtgagagg
     2041 cgtagatttt tatttgaaca aaggggaaac attggcaatt gttggtgaat caggttcagg
     2101 taaatctgta acaacaaaag caattacaaa attattccaa ggggacacag gaagaattaa
     2161 aaagggagaa attttatttt taggggaaga tttagcaaaa aaacctgaaa atgagttgat
     2221 taaattacgt ggcaaagata tttcaatgat ctttcaagat ccaatgacat ctttaaaccc
     2281 aacgatgcaa attggtaaac aagtcatgga accattaatt aagcacaaaa attatagtaa
     2341 agcacaagct aaaaagcgcg cattggaaat actaaatctt gtaggtttac caaatgcaga
     2401 aaaaagattt aaagcatatc ctcatcaatt ttcaggtgga caaaggcaaa gaattgttat
     2461 tgcaaccgca ttagcttgtg aacctaaagt gctcattgct gatgaaccaa cgactgcatt
     2521 agacgtaacg atgcaggcac aaattttaga tttaatgaaa gaactacaac aaaaaatcga
     2581 tacagcaatt atttttataa cgcatgattt aggggttgtt gcgaatattg ctgatagagt
     2641 ggcagttatg tatggtggtc aaatggttga aacaggagat gttaacgaaa tattttatga
     2701 tccaaagcat ccatatacat ggggattatt atcgtcaatg cctgatttat caacaacaaa
     2761 tgacacacca ttactagcga ttcctggagc gccacctgat ttattacacc cacctaaagg
     2821 tgatgcattt gcgagacgta gtcaatatgc attagatatt gattttaaag tagaaccacc
     2881 gtggtttaaa gtttcaccga cacattttgt gaaatcttgg ttattagacg cacgtgcacc
     2941 aaaagttgaa ctacccgagc tggtaaaaca acgtatgaaa ccgatgccta ataattatga
     3001 aaaaccactc aaggtagaaa gggtgtcgtt caatgaaaaa tgatgaagtg ctattatcta
     3061 ttaaaaattt aaagcaatat tttaacgcag gaaagaaaaa cgaagtgaga gcgattgaaa
     3121 atatttcgtt tgatatatac aaaggggaaa cattaggttt agtaggagaa tcggggtgtg
     3181 gtaaatctac aactggtaaa tcaattatta aacttaatga tattacaagt ggagaaattt
     3241 tgtatgaggg tattgatata caaaagattc gtaaacgtaa agatttgctt aaatttaata
     3301 aaaagataca gatgattttt caagacccat atgcgtcttt aaatcctagg ttaaaagtaa
     3361 tggatatagt agctgaaggt attgatatcc atcatttagc aactgataag cgtgaccgaa
     3421 aaaaacgtgt ctatgattta cttgaaactg ttggattaag taaagaacat gccaatcgct
     3481 atcctcatga attttcaggt ggacaacgcc aacgtattgg aattgcccgt gcattagccg
     3541 ttgaaccaga attcattatc gcggacgaac caatatcggc attggatgtt tcaatccaag
     3601 ctcaagtagt taatttatta ttaaaattac aacgtgaaag agggattacg ttcctattta
     3661 tagctcatga tctatcaatg gtgaagtata tttcagatcg tattgcagtc atgcattttg
     3721 ggaaaatagt tgaaattgga ccggcagaag aaatttatca aaatccatta cacgattata
     3781 ctaagtcttt attatcagcc attccacaac ctgatcctga atcagaacgc agtcgcaaac
     3841 gatttagtta tattgatgat gaagcaaata atcatttaag acaattacat gaaattagac
     3901 cgaatcactt tgtctttagt actgaagaag aagcggcaca actacgagaa aataaattgg
     3961 tgacacaaaa ttaaggggaa gggggaaatg caatgacgag aaaatttaga acacttattt
     4021 taattttgat tgctacaatt gcattaagtg gttgtgctaa tgacgatggt atttattcag
     4081 ataaaggtca agtattcaga aaaattttgt catcagactt aacatccctt gatacatcat
     4141 taataacgga tgaaatatct tctgaagtga ctgcgcaaac attcgaaggt ttatacacat
     4201 taggaaaagg tgacaaaccg gtgttaggtg ttgcgaaagc ttttcctgaa aagagtaaag
     4261 atggtaaaac tttaaaggtt aaattaagaa gcgatgctaa atggagcaat ggtgacaaag
     4321 tgactgcaca agactttgtt tatgcttgga gaaaaacagt tgaccctaaa acaggttctg
     4381 aatttgcata cattatgggg gacattaaaa atgcgagtga tattagtact ggtaagaaac
     4441 ctgtagagca attaggtatc aaagcattaa atgatgaaac attacaaatt gaattagaaa
     4501 agccggttcc atatattaat caattattag cacttaatac ctttgcacct caaaatgaaa
     4561 aagttgccaa aaaatatggt aaaaattacg gtacggcagc tgatagagcg gtatacaatg
     4621 gtccatttaa agttgatgat tggaaacaag aagataaaac cttactatct aaaaatcagt
     4681 attattggga taaaaagaat gtaaaattag ataaagtgaa ttataaagtt attaaagact
     4741 tacaagccgg tgcatcattg tatgatactg aatcagtaga tgacgcagtt attactgcag
     4801 atcaagtaaa taaatataaa gacaacaaag gattaaactt tgtgttaacg actgggacat
     4861 tttttgtaaa aatgaatgaa aaacaatatc ctgattttaa aaacaaaaat ttaagattgg
     4921 ctatcgcaca agcaatagat aaaaaaggat acgttgattc agtgaaaaac aatggctcaa
     4981 ttccttccga tacactaaca gccaaaggaa ttgcgaaagc gcctaatggc aaagattatg
     5041 cgagtaccat gaattcgcct ttaaaatata atcctaaaga agcaagagcg cactgggaca
     5101 aagctaaaaa agagttaggt aaaaatgaag tgacattttc aatgaacaca gaagatacac
     5161 cagatgcaaa aatatctgct gaatatatca aatcgcaagt tgagaaaaat ttaccaggag
     5221 ttactttgaa aattaagcaa ttaccgttta aacaaagagt atcactagaa ctgagtaaca
     5281 attttgaagc atcacttagt ggttggtctg cagattaccc tgatcctatg gcttatttag
     5341 aaacaatgac cacaggtagc gcacaaaata atacagactg gggtaataaa gaatatgatc
     5401 aattacttaa agtagcaaga accaaattgg cacttcaacc gaacgaacga tatgaaaact
     5461 tgaaaaaagc agaagaaatg ttcctaggag atgcaccggt agcaccaatt tatcaaaaag
     5521 gtgttgcaca tttaacaaat cctcaagtaa aaggattaat ttaccataaa tttggtccaa
     5581 ataactcact taaacatgta tatattgata aatcgataga taaagaaaca ggtaagaaga
     5641 aaaaataata tgctttgtaa attaggctgg agacatatct ccagtctttt tgtgttggat
     5701 aaaaactttg ggaataaaaa tttaaaataa gtcgtttttt aaattactga aattgattaa
     5761 atgcataaat aactgaatat tctaaaaata aacttgtaat aattttttct atgagtaaac
     5821 taaaaagaaa aaattagatt gaaagtagga ggcatatgta tggggaagct aattaaatat
     5881 atttcaatac ttcttattgt cgttttagtg ttgagtgctt gcggaaaaag cagtaataaa
     5941 gatgaaggag taaaagatgc tactaaaacg gaaacctcaa aacataaagg tggtacctta
     6001 aatgtagcat taacagcacc gccaagtggt gtttattctt cgttattaaa tagtacacat
     6061 gcagattctg tagttgaggg atattttaac gaaagcttat tagcaactga taaaaaaata
     6121 cgtcctaagg catatattgc ttcatggaag gacatcgagc cggctaagaa aatagaattt
     6181 aaaattaaaa aaggtattaa atggcatgat ggtaatgaat tgaaaattga tgattggatt
     6241 tattcaattg aagtcttagc taacaaggac tacgaaggtg cttattatcc aagtgtagaa
     6301 aatatccaag gtgcgaaaga ttatcatgaa ggaaaaactg atcatattag cggattgaag
     6361 aaaatagatg actacactat gcaggttaca tttgataaaa aacaagaaaa ttacttaaca
     6421 ggatttatta ctggaccttt attaagtaaa aaatatttat cagatgtacc aattaaagat
     6481 ttagcgaaat cagataaaat ccgaaaatat cctattggta ttggaccgta taaagttaag
     6541 aaaatcgttc caggtgaggc tgttcaactc gttaaatttg atgattattg gcaaggtaag
     6601 cctgcactag acaaaatcaa tttaaaagtt attgatcaag cgcaaattat taaggcaatg
     6661 gaaaaaggcg atattgatgt tgcgaatgat gctaccggtg caatggcaaa agatgctaag
     6721 tcatctaatg ctggtctcaa ggtattatct gcgccaagct tagactacgg tttaatagga
     6781 ttcgtatctc atgattacga taaaaaagct aataaaactg gtaaagtgag accaaaatat
     6841 gaagacaaag aattacgtaa agcaatgctt tatgcaattg atagagaaaa atggatcaaa
     6901 gcgtttttca atggttacgc tagtgaaatc aatagttttg taccatctat gcattggata
     6961 gcagccaatc ctaaggacct aaatgattac aaatatgatc ctgaaaaagc taaaaaaatc
     7021 ttagataagt taggttataa agatagagat ggtgacggat ttagagaaga tcctaaaggt
     7081 aataaatttg agattaactt taaacattat tcaggttcta atcctacttt tgaaccaaga
     7141 actgctgcga taaaagattt ctgggaaaaa gttggcttga aaacaaatgt gaagttagta
     7201 gaattcggta aatataatga agacttagca aatgcatcta aagatatgga agtgtacttc
     7261 agatcatggg caggaggtac agatccagat ccatcagatt tataccacac tgatagacct
     7321 caaaatgaaa tgagaacagt tttaccaaaa tcagatcaat atttagatga tgcattagac
     7381 ttcgaaaaag taggcattga tgaaaagaaa cgtaaagata tttatgttaa atggcaaaaa
     7441 tatatgaatg atgagttacc tggattacca atgttccaag gtaaatcgat aactattgtt
     7501 aacgataaag tacgaaactt agacattgaa attggaactg atcaaagttt atataattta
     7561 actaaagaag cttagtaatg taacctcgac gattttgtcg aggtttactt atatggggga
     7621 tgttatgaat aatgtattgt tagaggttaa agatttagaa acatcattaa aaataaataa
     7681 tgaatggtta gcaactgttg aaaatatttc ttttgaatta tctaaaggag aagttttggg
     7741 tatagtaggg gaatctggtt gcggtaagtc catattaagt aagtcaatta ttaaattatt
     7801 accagaaaag atatctaaac taagtaatgg agaagttata tttgatggta aacgaatcga
     7861 tacgctcaat gagaagcaat tgccagatat tcgaggaaat gatattgcta tgatttttca
     7921 agaacctatg actgctttaa atcctgtatt taccataaaa aatcaacttg tggaatctat
     7981 aaaatcacat aaaaaaattt ctaaaaaaga agcaaataaa ttagcaaaag atttactaaa
     8041 aaaagttgga attgctagac aagatgaaat attaaatagc tatcctcatc aattatctgg
     8101 tggtatgaga caaagagtaa tgattgcaat ggccatttca tgttctccta aattattaat
     8161 tgctgatgaa cctacaacag cattggatgt cacgattcaa gcgcaaatat tagacttatt
     8221 aaaagaattg caaaaggaaa cgcaaatggc aattatgatg attacacatg atttgagtgt
     8281 agttgctgag ttttgcgata aagtcttagt tatgtatgca ggtcaaattg tagaatttgg
     8341 aggcataaaa gaaatactac acaatccgaa acatccttat acccaaaaat tattatcaac
     8401 aattccaaaa cttaaagaag agcagaaacg acttgaaacg atagaaggaa ttgtgccatc
     8461 aatccaagca tttcacgtta ataagtgcag atttgcaaat agatgtaaca aaaaactgga
     8521 tatttgtaat aatcaatctc ctaaaatgca tgtttgtgaa gatgtcattg tacgttgtca
     8581 tttgtacaaa aatgaatata aggagatata agaatggaaa atattttaga agtcaaccaa
     8641 ataaaaaaat actacaaaat taaaactgga ttattacaaa aaactcagta cgttaaagct
     8701 gttgatgacg tatcgttttc aataaaaaaa ggacaaactt ttggattagt aggagaatcg
     8761 ggttgtggta agtcaacgtt aggtaaagtg attatcaggc ttgaagatgc aacttcaggc
     8821 tcaataattg ttaatggtga agatataaca agattacaag gtaaaaaact cagaaaatca
     8881 cgacaacaat atcagatgat atttcaagat ccgtatgcat cattgaatcc gatgcaaatg
     8941 gttggagata tcatttcaga acctatttta aattataaaa aattgccaaa agaagaaata
     9001 aaaaaagaag tactatattt attaaaatgt gttggcctaa gtgaagatgc atattataaa
     9061 tatgcacatg aattttcagg tggacagaga caaagagtgg gaattgcaag agcattggct
     9121 ttgcgtccga gtttaattgt tgctgatgag cctgtaagtg cattagatgt atctgttcaa
     9181 tctcaagtac tgaatttatt aaaagattta caagaacaat ttaacttaag ctatttattt
     9241 atcgcacatg atttaagtgt agtaaaacat ataagtgatg tcattggagt tatgtattta
     9301 ggtcatatag ttgaaatcgc atctgataaa gaaatttatg aaaatcccaa acatccatat
     9361 acaaaagcgt tgatttcatc aataccacaa attgataaac ataataacaa tagaattata
     9421 ttaaaaggag aattaccttc gccaagtaat ccgccgcaag gttgtccttt tcatacaaga
     9481 tgtccaattg ctcaagatat gtgcaaaaaa agtatgccag aattaaagga cattggtaat
     9541 gaacatcaag ttgcttgttt ctacgtagat aaagtaggtg atttaaatga ttaaattaat
     9601 acttaaaaga ttagggttaa tgatcccgtt actaatttta atctcaatag ttgtattttc
     9661 attagcaatc atacaaccag gagatccatt ttcagatcta caaaacggta aaataaaaca
     9721 agaagcaata aatgcacaaa gggaaaaatt aggtctcaac gactcaatac cacatcaata
     9781 cattagatgg gtcaatcatg ttatacacgg tgatttaggt gagtcaatca aatataaaag
     9841 gccggtaatt gatgttattg aggaaagaat tccaaataca atattactcg gtgctatgtc
     9901 attaattatt acttatatta tctcatttgc tttaggaata acgtcaggta gatattctta
     9961 cagtttgacg gattatactg tgcaaatatt taattatttg atgttagcca ttccatcttt
    10021 tattgcggga gtatttgcaa tttttatttt ttcttttgaa ttacaatggt tcccgtttca
    10081 aggttctgtt gatattaacc ttaaagaagg tacttttgaa tattatatga gtaaaatata
    10141 tcacacattt ttacctgcat tcactttagg tttattatct actgctggtt atattcaata
    10201 tttacgtaat gatattattg aaaattctaa aaaagattat gtattgacgg caaggtcaaa
    10261 aggattatct atgaataaaa tttataataa acatatattg agaaattctc taatacctat
    10321 tattacattt ttaggtgctg atattgtaag tattttaggt ggagccgtga ttacggagac
    10381 tatcttttca tataacggta tcggtaaatt atttttagaa tcggtaatag gccaagacta
    10441 tccattaatg atggcattaa cgttattttt ctcattttta ggtttactgg gtaatttgat
    10501 ttctgatatt acttatggat ttatagatcc aagaattaga agtaactagg gggattagaa
    10561 tgcaaaataa gtcaaaatcg ccttttaaaa ttgcattttc taaatttatt cataataaaa
    10621 ttgcaatgtt atcggttatt tttttattaa tcataactat tgtatcaatt atagcgccat
    10681 taatagctcc ttttccagtg aaccaacaag atttattaaa tataaaaggt gaaatgacag
    10741 cacaaaacat tcttggtaca gactctggtg gtagggataa ctttagtcgt ttgttatatg
    10801 caggtcgtat ttcattatcc attggaatta catctacaat aggaatgctt ttgattggaa
    10861 ttacagttgg agtgatttct ggttattttg gaggtattgt tgatacatta ttaatgagaa
    10921 taaccgaatt tgttatgtta tttccatttt taatatttgc aattgtatta aatgctgcac
    10981 ttggagataa aattaaaaat ccttatggat ctgccataat tcttgttcta gttattatcg
    11041 tattaagttg gggaggtatt gcaagacttg ttcgtggtaa agtacttcaa gaaaaagaaa
    11101 atgaatactt tttggcagca aaatcaattg gtacacccac atataaaatt attttgaaac
    11161 atcttttgcc gaatatatta agtgtagtta tcgtacaagc aacattgtta tttgccggta
    11221 tgattgtagt ggaatcagga ttgagctttt taggattcgg aattagtaaa gcaataccat
    11281 cttggggtaa tatgttgagt gatgctcaag aaggggatgt tataagtggt aaaccgtgga
    11341 tatggatgcc acctgctata atgattacat taactatatt aagtataaac tttgtagggg
    11401 aagggattaa agatgctttt aatcctagag gtagacgtta aataataaaa gaggcactag
    11461 ttaattctag tacctcttta ttttatctct tacgtcctaa acccatcgct ttttccattt
    11521 ttttgacagt tttaaatgaa actttgtgtg ctttatctct accttgatct aaaatatcat
    11581 caagtttatc tgagttatag aaactttcgt atttttcttg gaattctact aaaaatgctt
    11641 taactatttc agcaaggtca cctttaaatt taccataacc ttcgccctca tattttgcct
    11701 caatatcttt aattggcatg tctgttaatc cagcgtatat tgaaattaaa tttgttatac
    11761 ctggcttgtt gtcacgatca aatttaataa taccatctga atcagttact gcgcttttaa
    11821 tttttttagc tgcaacattc ggctcgtcta ataatgaaat gaagttttta gcattatcat
    11881 cactcttact catttttctt gttgggtctt gtaaactcat gacacgtcca ccaactttag
    11941 gcatacgaat ttcaggtttc acaagcacat cattatagcg actattaaat ctatctacaa
    12001 ggttacgagt caattcgata tgctgctttt ggtcatctcc aactggaacg atattagtat
    12061 tgtaaagaac aatatcagct gccattaaag gtggatatgt taatagacca gcaggtatac
    12121 cttcaactgc tttctgagct ttatctttgt attgcgtcat acgctctaat tctccaacag
    12181 aagcaatcgt agttaacatc catcctgctt gtacgtgtgc agggacttca gattgtatga
    12241 acaatgttgc tttgtctgga tctataccag aagctaaata aatcgctgct aattgtctgg
    12301 tctgtttacg taattttaaa cgatcttgtg gcattgtaat tgcatgttga tctacgatac
    12361 agaaataaca atcatagtca ttttgcacat caacaaattg ttttagtgcg ccaatataat
    12421 ttccaatagt aggaattcca ctaggttgga tgcctgaaaa taatgtctcc atttaaatgc
    12481 ctactttctt agtaatattt taataattat cattataaca gtatttctta aaaatgtaag
    12541 atgtgttaga atatacgtat aacttactac taacaaaggg attaataatc ttttttataa
    12601 gtgtagaaac ttatctgaaa aattaggttt ttctcattta attttaatgt tagatagtta
    12661 taataaatag taacgattaa gaattttaga aaaaaatgtt ttttcttgat ctataactaa
    12721 attttaagca atgaatttta ttcattcaaa taggagagtg agatgtatgg taacattatt
    12781 tacttcacca agttgcacat cttgccgtaa agcgaaagca tggttacaag aacatgacat
    12841 tccgtatacg gagcgtaata ttttttctga acatttaaca attgatgaaa ttaagcaaat
    12901 attaaaaatg actgaagacg gtactgatga aatcatttct acacgttcta aaacatacca
    12961 aaaattaaat gttgatattg attcactacc attacaagac ttatattcaa tcattcaaga
    13021 taatcctggc ttattacgtc gtccaattat tttagataat aaacgactac aagttggtta
    13081 taatgaggac gagattcgac gtttcttacc tagaaaagtt cgtacgttcc aattacaaga
    13141 agcacaacgt atggttgact aataaccatt taactatgtg tcatacttta ctaaaagata
    13201 tgtcaagcaa cactatttct atagtgttgc ttttttggtt ttaattttac aataattgct
    13261 tagtatcttt taaatcaaat gtgcaattgt taatatagct ctatattaga gtgttttgga
    13321 tgaaacattt gctatgataa ggtaaaatta aattaagaaa gtgct
//
LOCUS       DALAGD010000081        13286 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.082, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000081 DALAGD010000000
VERSION     DALAGD010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13286
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.082"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(622..819)
                     /locus_tag="O2701_002048"
     CDS             complement(622..819)
                     /locus_tag="O2701_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293930.1"
                     /translation="MNCYDEIFNTIKELIENKEISSYQINKDTGISYGNINAMRRRER
                     RIENLSLKNAKILYEYAKKVL"
     gene            complement(1312..2652)
                     /gene="glnA"
                     /locus_tag="O2701_002049"
     CDS             complement(1312..2652)
                     /gene="glnA"
                     /locus_tag="O2701_002049"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HCZ4293931.1"
                     /translation="MPKRTFTKDDIRKFAEEENVRYLRLQFTDILGTIKNVEVPVSQL
                     EKVLDNEMMFDGSSIEGFVRIEESDMYLHPDLDTWVIFPWTAGQGKVARLICDVYKTD
                     GTPFEGDPRANLKRVLKEMEDLGFTDFNLGPEPEFFLFKLDEKGEPTLELNDDGGYFD
                     LAPTDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKL
                     VVKTIARKHNLHATFMPKPLFGVNGSGMHFNVSLFKGKENAFFDPNTEMGLTETAYQF
                     TAGVLKNARGFTAVCNPLVNSYKRLVPGYEAPCYIAWSGKNRSPLIRVPSSRGLSTRI
                     EVRSVDPAANPYMALAAILEAGLDGIKNKLKVPEPVNQNIYEMNREEREAVGIQDLPS
                     TLYTALKAMRENEVIKKALGNHIYNQFINSKSIEWDYYRTQVSEWERDQYMKQY"
     gene            complement(2671..3039)
                     /locus_tag="O2701_002050"
     CDS             complement(2671..3039)
                     /locus_tag="O2701_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCZ4293932.1"
                     /translation="MISNDAIRRNMAVFSMSVVSKLTDLTPRQIRYYETHELIKPERT
                     EGQKRLFSLNDLERLLEIKSLLEKGFNIKGIKQIIYDSQEHLTTDEQEIRKKMIVDAT
                     QKPIGETLPINRGDLSRFIK"
     gene            complement(3282..4520)
                     /locus_tag="O2701_002051"
     CDS             complement(3282..4520)
                     /locus_tag="O2701_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCZ4293933.1"
                     /translation="MKDISKIVADVESTLAPYFKEIEETAYINQEKVLNAFHHVKATE
                     SDLQGSTGYGYDDFGRDHLEEIYAQAFKAEDAIVRPQIISGTHAITIALQSLLKHGDE
                     LIYITGSPYDTLLEVIGVNGNGIESLMEHGVSYKDIALKEGKIDIESVLDGVSERTKV
                     IAIQRSKGYDQRPSIPLDEIEKVITRLKNVHPNILIFVDNCYGEFVERREPIECGADL
                     IAGSLIKNPGGGLAKIGGYIAGRKDLIERCGYRLTAPGIGKEAGASLNALLEMYQGFF
                     LAPHVVSQSLKGALFTSLFLEKMNMNTTPKYYEKRTDLIQTVKFETKEQMISFCQSIQ
                     HASPINAHFSPEPSYMPGYEDDVIMAAGTFIQGSSIELSADGPIRPPYEAYVQGGLTY
                     EHVKIAVTRAVNQLKEQGLI"
     gene            complement(4539..5777)
                     /gene="hflX"
                     /locus_tag="O2701_002052"
     CDS             complement(4539..5777)
                     /gene="hflX"
                     /locus_tag="O2701_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCZ4293934.1"
                     /translation="MAQQQIHDTKNKLEKAVLVGVHAQDDKQFNFESTMEELSSLSET
                     CQLEVLGQITQNRDRVDRKYYVGKGKIEEIQAFIEFKDIDVVITNDELTTAQSKSLNE
                     ALGVKIIDRTQLILEIFALRARSKEGKLQVELAQLDYLLPRLQGHGKSLSRLGGGIGT
                     RGPGETKLEMDRRHIRTRMNEIKHQLRTVEEHRERYRNKRNQNQVFQVALVGYTNAGK
                     SSWFNVLANEETYEKDQLFATLDPKTRQIQINDGFNLIISDTVGFIQKLPTTLIAAFK
                     STLEEAKGADLLVHVVDSSHPEYRTQYDTVNDLIKQLDMSHISQIVIFNKKDLCDHAS
                     NRPASDLPNVFVSSKNDGDKLLVKTLFIDEIKRQLTYYDETIATNNADRLYFLKQHTL
                     VTELKYDEIENVYRIKGFKK"
     gene            5889..6365
                     /locus_tag="O2701_002053"
     CDS             5889..6365
                     /locus_tag="O2701_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCZ4293935.1"
                     /translation="METIYDFVVETNKGVTYKLDAYKGDVMLIVNTASECGFTSQFEG
                     LQSLYEKYKDQGFVILGFPCNQFGGQEPGSGEEAAQNCKLNYGVTFPMHQKIDVKGEH
                     QLPLFRYLTAAQHGFFNEKIKWNFTKFLVDREGNVVKRFAPQKKPVQIEREIEKLL"
     gene            complement(6587..6820)
                     /gene="hfq"
                     /locus_tag="O2701_002054"
     CDS             complement(6587..6820)
                     /gene="hfq"
                     /locus_tag="O2701_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCZ4293936.1"
                     /translation="MIANENIQDKALENFKANQTEVTVFFLNGFQMKGVIEEYDKYVV
                     SLNSQGKQHLIYKHAISTYTVETEGQASTESEE"
     gene            complement(6835..7770)
                     /gene="miaA"
                     /locus_tag="O2701_002055"
     CDS             complement(6835..7770)
                     /gene="miaA"
                     /locus_tag="O2701_002055"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCZ4293937.1"
                     /translation="MNKNKPFIVVIVGPTASGKTELSIELAKRINGEIISGDSMQVYK
                     HMNIGTAKVTPEEMDGIPHHLIDILNPDDTFSAYEFKRLAEDLITDITNRGKVPIIAG
                     GTGLYIQSLIYNYELEDETVTPAQLSIVKQKLSALEHLDNQQLHDYLAQFDAVSAENI
                     HPNNRQRVLRAIEYYLKTKKLLSNRKKVQQFTENYDTLLLGIEMSRKTLYSRINKRVD
                     IMLDHGLFREVQQLVEQGYESCQSMQAIGYKELIPVINGQMIYEDAVNDLKQHSRQYA
                     KRQMTWFKNKMSVHWLDKENMSLQMMLDEITTQIK"
     gene            complement(7788..8702)
                     /locus_tag="O2701_002056"
     CDS             complement(7788..8702)
                     /locus_tag="O2701_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCZ4293938.1"
                     /translation="MTEKQFKLTVQDNTNIEVKVNFTDVDSKGIIHIFHGMAEHMERY
                     DKLAHALSKHGFDVIRHNHRGHGINIDESTRGHYDDMKRVIGDAFEVAQTVRGNVDKP
                     YIIIGHSMGSVIARLFVETYPQYVDGLILSGTGMYSLWKGLPTVKVLQLITKIYGAEK
                     RVEWVNQLVSNSFNKKIRPLRTQSDWISSNPIEVDNFIKDPYSGFNVSNQLLYQTAYY
                     MLHTSQLKNMKMLNHAMPILLVSGYDDPLGDYGKGILKLANIYRKAGIKNVKVNLYHH
                     KRHEVLFEKDHDKIWEDLFKWLNQFYKK"
     gene            complement(8852..10525)
                     /locus_tag="O2701_002057"
     CDS             complement(8852..10525)
                     /locus_tag="O2701_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HCZ4293939.1"
                     /translation="MALSTFKREHIKKNLRNDEYDLVIIGGGITGAGIALDASERGMK
                     VALVEMQDFAQGTSSRSTKLVHGGLRYLKQFQIGVVAETGKERAIVYENGPHVTTPEW
                     MLLPMHKGGTFGKFSTSIGLGMYDRLAGVKKSERKKMLSKKETLAKEPLVKKEGLKGG
                     GYYVEYRTDDARLTIEVMKRAAEKGAEIINYTKSEHFTYDKNQQVNGVKVIDKLTNEN
                     YTIKAKKVVNAAGPWVDDVRSGDYARNNKKLRLTKGVHVVIDQSKFPLGQAVYFDTEK
                     DGRMIFAIPREGKAYVGTTDTFYDNIKSSPLTTQEDRDYLIDAINYMFPSVNVTDEDI
                     ESTWAGIRPLIYEEGKDPSEISRKDEIWEGKSGLLTIAGGKLTGYRHMAQDIVDLVSK
                     RLKKDYGLTFSPCNTKGLAISGGDVGGSKNFDAFVEQKVDVAKGFGIDEDVARRLASK
                     YGSNVDELFNIAQTSQYHDSKLPLEIYVELVYSIQQEMVYKPNDFLVRRSGKMYFNIK
                     DVLDYKDAVIDIMADMLDYSPAQIEAYTEEVEQAIKEAQHGNNQPAVKE"
     gene            complement(10683..12179)
                     /gene="glpK"
                     /locus_tag="O2701_002058"
     CDS             complement(10683..12179)
                     /gene="glpK"
                     /locus_tag="O2701_002058"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCZ4293940.1"
                     /translation="MEKYILSIDQGTTSSRAILFNQKGEIAGVAQREFKQYFPQSGWV
                     EHDANEIWTSVLAVMTEVINENDVRADQIAGIGITNQRETTVVWDKHTGRPIYHAIVW
                     QSRQTQSICSELKQQGYEQTFRDKTGLLLDPYFAGTKVKWILDNVEGAREKAENGDLL
                     FGTIDTWLVWKLSGKAAHITDYSNASRTLMFNIHDLEWDDELLELLTVPKNMLPEVKA
                     SSEVYGKTIDYHFYGQEVPIAGVAGDQQAALFGQACFERGDVKNTYGTGGFMLMNTGD
                     KAVKSESGLLTTIAYGIDGKVNYALEGSIFVSGSAIQWLRDGLRMINSAPQSESYATR
                     VDSTEGVYVVPAFVGLGTPYWDSEARGAIFGLTRGTEKEHFIRATLESLCYQTRDVME
                     AMSKDSGIDVQSLRVDGGAVKNNFIMQFQADIVNTSVERPEIQETTALGAAFLAGLAV
                     GFWESKDDIAKNWKLEEKFDPKMDEGEREKLYRGWKKAVEATQVFKTE"
     gene            complement(12308..13126)
                     /locus_tag="O2701_002059"
     CDS             complement(12308..13126)
                     /locus_tag="O2701_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCZ4293941.1"
                     /translation="MNVYLAEFLGTAILILFGGGVCANVNLKRSAANGADWIVITAGW
                     GLAVTMGVFAVGQFSGAHLNPAVSLALALDGSFDWSLVPGYIVAQMLGAIVGATIVWL
                     MYLPHWKATEEAGAKLGVFSTAPAIKNYFANFLSEIIGTMALTLGILFIGVNKIADGL
                     NPLIVGALIVAIGLSLGGATGYAINPARDLGPRIAHAILPIAGKGGSNWSYAIVPILG
                     PIAGGLLGAVVYAVFYKHTFNIGCAIAIVVVIITLILGYILNKSSKKGDIESIY"
BASE COUNT         4100 a         2560 c         1831 g         4795 t
ORIGIN      
        1 gatagagagt taattttttc cttaattctc ttgtcataaa taccacctcc ctatttaatg
       61 tggtaaaggt cttttgccct ttaccttttt aaaagaacga tttgataaaa ttaagtactt
      121 gaattattaa gctcgtcaca gctattcgaa atttaatttt gctattcgtt ctttttcttt
      181 aaccattctt atcatttttt ctcctttcct ctttatagtt atttaacatt ccacaaattg
      241 gggtaagtga catgttgaac tcccatatcc actggcaaga tttataattt gtaattctaa
      301 agtataatag cctatatttt tcaaaatctc tttttttatg attaacatcc ctctaagtgc
      361 acctaaaggt ttttatgtaa tgtattaaat atttccattt atacatatac atcttgtata
      421 aaaagaaaga actccatata tactcaaaag gtatactgaa gctctatata ttatataaaa
      481 ttattatact attttgaaaa acataagtaa atcactaaac atgatttctt tcacttttag
      541 aaaactttta atactataaa agcacccact cagtcactag tttgggcagt tattgtatgc
      601 ctattgaact caatgcttat attacaatac ctttttcgca tattcatata agatctttgc
      661 attctttaag cttaaatttt ctattcttct ttctctacgg cgcatagcat taatattacc
      721 gtaacttatc ccagtatctt tattaatttg ataactcgat atctctttgt tttctatcaa
      781 ttctttgatt gtattgaata tttcatcata gcaattcata aattagatga ggcgaaattt
      841 ttaatttttt agaatatcaa tagtagtata actaaaatga aaataccgat cgataaacaa
      901 aaagatattt tttgttttgt ttctcttttc atatagtatt acccccttaa taatgcgtag
      961 taaggtccct cttttcgggg tcttacctta gaacgttctg caaatgaatt cgatgagaag
     1021 taatatgaat atggctattt tcaagtaata ctcaacgttt tcgcgacgtt cttttatcgc
     1081 ctcatctcat cacctccaaa tatattaaaa ttcatgtgaa ctaaaatata aaatggtctt
     1141 ccccagcttt aaaaaaataa atacataaaa catttacttg accaaaactt gaccaaatcc
     1201 attttttaac aaaagtggtc aagtgaaatc ccatctttcc aaatcaaaaa agagctaaag
     1261 caaaatgctc tagcccttga tattactgat ttcccatcat tgtttagcgt attaatattg
     1321 cttcatgtac tgatctcttt cccattcaga gacttgagtt ctgtagtaat cccattcaat
     1381 tgattttgaa ttaataaatt gattatagat atgatttcct aaagcttttt taataacttc
     1441 attttcacgc attgctttta atgcagtata aagtgttgaa ggtaagtctt gaatgcctac
     1501 tgcttcacgt tcttcacggt tcatttcgta aatattttgg ttaactggtt ctggaacttt
     1561 taatttattt ttaataccat ctagtccagc ttctaagatt gcagctaacg ccatgtatgg
     1621 gtttgcagct ggatctactg aacgtacttc gatacgagta gataatcctc ttgaagatgg
     1681 tacacggatt aatggtgaac ggtttttacc actccatgca atataacatg gtgcttcata
     1741 accaggtact aaacgtttat atgagtttac taacgggtta catacagcag taaatccgcg
     1801 tgcattttta agcacacctg ctgtaaattg atatgcagtt tccgttaagc ccatttcagt
     1861 atttggatca aagaatgcat tttctttacc tttgaataat gaaacgttaa agtgcatacc
     1921 gctaccattc acaccgaata atggtttagg cataaatgtt gcgtgtaaat tatgtttacg
     1981 tgcgattgtt ttaacaacca atttaaatgt ttggatatta tcacatgctg taacagcatc
     2041 tgcatattta aagtcaattt catgttgacc aggggcaact tcatggtgac tagcttcaat
     2101 atcgaagccc atatcctcta attctaaaac aatatcacga cgacagtttt cacctaaatc
     2161 tgtaggtgct aaatcgaaat atccaccatc atcattaagt tctaaagttg gttccccttt
     2221 ttcatccaac ttaaacaaga agaattctgg ttcaggccct aggttaaagt ctgtgaagcc
     2281 taaatcttcc atttctttta atacacgttt taagtttgca cgaggatccc cttcaaatgg
     2341 tgttccatct gttttatata catcacaaat taaacgtgca actttacctt gtccagcagt
     2401 ccatgggaag attacccaag tatctaaatc tggatgtaag tacatatctg attcttcgat
     2461 acgtacgaaa ccttcgatag aagaaccgtc aaacatcatt tcgttatcaa gtactttttc
     2521 taattggctt acaggcactt caacattttt aattgttcct aaaatatcag tgaattgtaa
     2581 tcttaaatat cttacatttt cctcttctgc aaatttacga atgtcgtctt tagtgaaagt
     2641 acgttttggc attttaaaat cctccaaatt ttatttaata aatcgggata aatcaccacg
     2701 atttattggc aaagtttctc caataggctt ttgcgtggca tctacaatca tcttttttct
     2761 tatctcttgt tcatctgttg ttaaatgctc ttgtgagtca taaatgattt gtttaatccc
     2821 tttgatatta aatccttttt ctaataatga tttaatttct agtaatcttt ccaaatcatt
     2881 gagtgagaac agacgttttt gaccttctgt tctttcaggt ttgatgagtt catgtgtttc
     2941 atagtaacgt atttgccttg gcgttaaatc cgttaactta cttactacac tcatagagaa
     3001 gacagccata tttcgtctga ttgcatcatt cgatatcatt gttcctctcc tctacttttg
     3061 aacttgttaa taacttatca taattttttt aggatttcaa aaatatgtca ggttttctaa
     3121 cataaagctt aaaacattga tataactgcg atttcgactt ctaaaacgac ataaaacaat
     3181 ctacagctaa aatatttttt caatttttaa aaataatctg ataaatctcc gcaaaaaatt
     3241 tcacccaaaa cacattatca tttagggtga aatttaatct attatataag tccttgttct
     3301 ttcaactgat taacagctct tgtcacagca attttaacgt gttcatatgt taatcctcct
     3361 tgaacatatg cttcataagg aggacgaata ggtccatctg cagataattc aatcgatgaa
     3421 ccttgaataa acgtaccagc tgccataata acatcatctt cgtaaccagg catataacta
     3481 ggttctggac taaaatgtgc attaattggg gatgcgtgtt gaatactttg acaaaatgaa
     3541 atcatttgtt ctttcgtttc aaatttaact gtttgaatta aatcagttcg tttttcgtag
     3601 tacttcggcg ttgtgttcat attcattttt tctaaaaata aactagtaaa caatgcacct
     3661 ttaagactct gactgacaac gtgtggtgct aaaaagaaac cttgatacat ttcaagcaat
     3721 gcatttaatg atgcacccgc ttctttacca ataccaggtg ctgtcaatct ataaccacat
     3781 cgttcaatta aatcttttct accagcaatg tatccaccaa tcttagctaa accaccgcca
     3841 gggtttttaa ttaatgatcc tgctattaaa tcggcaccac attctatagg ttcacgtctt
     3901 tcaacaaatt ccccataaca gttatccaca aatattaaaa tattaggatg cacgtttttc
     3961 aacctagtaa ttaccttttc aatttcatct agcggaattg aaggtctttg atcatagcct
     4021 ttcgaacgtt gaatcgctat tactttggtg cgctcagaaa ccccatctaa cacactttca
     4081 atatcgatct taccttcttt aagtgcaata tctttatacg atacgccatg ctccattaaa
     4141 ctttcaatac catttccgtt tacgccaatg acttcaagta aagtgtcata tggactaccc
     4201 gttatataaa ttaattcatc accatgtttt aataaacttt gtaatgcaat agtaatcgca
     4261 tgcgtacctg aaataatttg cggacgaaca attgcatctt ctgctttaaa tgcctgcgca
     4321 tatatttctt ctaaatgatc acgtccaaag tcatcatacc cgtatcctgt tgatccttgt
     4381 agatcacttt cggttgcttt gacatgatga aatgcattta atactttttc ttgattaata
     4441 tatgctgttt cttcaatttc tttaaaatat ggtgctaacg ttgattcgac gtcagctact
     4501 atcttactta tatctttcat ttgaatttcg tccttttatt attttttaaa tccttttata
     4561 cgataaacat tttcaatttc atcatattta agttcagtca ctaatgtatg ttgttttaga
     4621 aaatataatc gatctgcatt attcgtcgca attgtctcat cataataagt taattgcctt
     4681 ttgatttcat caataaataa cgtcttaaca agtaatttat caccatcatt tttagaagaa
     4741 acaaaaacat taggcaaatc acttgctgga cgatttgatg catgatcaca taagtccttt
     4801 ttattaaaaa taactatttg agaaatatga ctcatatcta attgtttgat taaatcatta
     4861 actgtgtcat actgcgtacg gtattcagga tggctactat ctacgacatg tactaataaa
     4921 tctgcacctt tagcctcttc taaagttgat ttaaaagctg caattaacgt cgtaggtagt
     4981 ttctgtataa aaccaacagt atctgaaata attaaattaa atccatcatt tatttgaatt
     5041 tgtcgtgttt taggatctaa cgttgcaaat aattgatctt tttcatacgt ctcttcattt
     5101 gctaaaacat taaaccatga tgatttacca gcatttgtat aaccaactaa agctacttga
     5161 aacacctgat tttgatttct tttatttcga tatctttcgc gatgttcttc taccgtccgc
     5221 aattgatgtt taatttcatt catacgagtt cgaatatgtc tgcgatccat ctctaacttc
     5281 gtttcaccag ggcctctagt tccaataccg ccacctaaac gagaaaggct tttaccatgg
     5341 ccttgcaatc taggtaataa ataatcaagt tgtgctagct ctacttgcaa tttaccttct
     5401 ttacttcttg ctcttaatgc aaatatttca agaatcaact gagttctatc aataattttt
     5461 acacctaaag cttcatttag tgatttggat tgtgcagtcg ttaattcatc atttgtgatg
     5521 actacatcaa tatctttgaa ctcaataaat gcttgaattt cttcaatttt acctttacca
     5581 acataatatt tgcgatctac acgatctctg ttttgagtaa tttgacccaa cacttcaagt
     5641 tggcaagtct ctgataaaga tgataattcc tccattgtag actcaaaatt aaattgctta
     5701 tcatcttgag catgtacacc gactaagaca gctttttcta gtttattttt agtatcatga
     5761 atttgttgct gagccatgtt atacatctcc ttaataaaat cctactcaaa gtattctatc
     5821 atagagtttt agtaaagact attacacgat tttgtggtac attttaagta aatcgaaagg
     5881 atgatacaat ggagacaatt tatgattttg tagtagaaac aaataaaggt gttacttaca
     5941 aattagatgc atataagggt gacgttatgc taattgttaa tacagctagc gaatgtggct
     6001 ttacttcaca atttgaaggt ttgcaatcat tatacgagaa atacaaagat caagggtttg
     6061 taattctagg tttcccttgt aatcaatttg gtggccaaga acctggttca ggcgaagaag
     6121 cagctcaaaa ctgtaaatta aactatggtg ttaccttccc tatgcatcaa aaaatagacg
     6181 taaaagggga acatcaatta cctttattta gatatttgac tgcggcacaa cacggattct
     6241 ttaatgagaa aatcaaatgg aatttcacta agtttttagt ggatcgcgaa ggtaacgttg
     6301 ttaaacgatt tgcacctcaa aaaaaacctg ttcaaatcga aagagaaata gaaaaattat
     6361 tataaaatat actagctcat agcaaataaa acgcttcata taaagtcgta tcatatattg
     6421 actcgacctt tatatgaagc gtttttatta caaacattcc tctattgtat cttgtcattt
     6481 gaacatcatt taattgatgt cacatatgac tttatttcta tatctattta tcatacttca
     6541 aacgttatgt acacgagtca ttaaatcgtg cccatatgat tacaacttat tcttcacttt
     6601 cagtagatgc ttgaccttca gtttctactg tataagtgct gatcgcatgt ttgtaaatca
     6661 agtgttgttt gccttgagaa tttaagctta cgacatactt gtcgtattct tcaataacac
     6721 ctttcatttg gaaaccgttt agaaagaata cagttacttc agtttggttt gctttaaaat
     6781 tctctagtgc tttgtcttgg atgttttcgt ttgcaatcat ctgtcggact ccttttactt
     6841 aatctgggtt gtaatctcat ctaacatcat ttgaagtgac atattttctt tatctaacca
     6901 atgaacactc attttattct tgaaccatgt catttgtcgt tttgcatatt ggcgtgaatg
     6961 ttgctttaaa tcattgacag catcttcata aatcatttgt ccgttaatca caggtattaa
     7021 ttctttatat ccaatagctt gcatactttg gcaagattca tagccttgtt caacaagttg
     7081 ttgcacttct ctaaataatc cgtgatccaa cataatatca acacgtttat ttattcttga
     7141 atataatgtt ttacgcgaca tttcaatccc taataataat gtatcataat tttcagtaaa
     7201 ttgttgcact ttcttgcgat tactcaaaag tttttttgtt tttaaataat attcaatagc
     7261 gcgcaacact ctttggcggt tgttagggtg aatattttct gcagaaaccg catcaaattg
     7321 agctaaataa tcgtgtagtt gctgattatc taaatgttct aatgcagata acttttgttt
     7381 aactatggat aattgtgcag gtgtaactgt ttcatcttct aattcataat tatatattaa
     7441 tgattgaata tataagcctg ttccacctgc tatgattgga actttacctc tattcgttat
     7501 atcagtaatt aaatcttctg ctaatcgctt gaattcatat gctgaaaatg tatcatcagg
     7561 attcaagata tcaattaaat gatgtggaat accatccatt tcttcaggtg ttacttttgc
     7621 agttccaata ttcatatgtt tgtagacttg catagagtca ccgcttatga tttcaccatt
     7681 gatacgcttc gcgagttcga tgctaagctc tgttttacct gaagcagttg gccccacaat
     7741 tactacaata aaaggcttat ttttattcat atttaattcc actttcttta ttttttataa
     7801 aattgattca accatttaaa caagtcttcc caaattttgt catgatcttt ttcaaataac
     7861 acttcatgac gtttatgatg ataaagattc actttaacat ttttaatgcc agcttttcta
     7921 tatatattcg ccaattttaa aatcccttta ccataatcac ctaaaggatc gtcatatcct
     7981 gaaactaata atataggcat ggcatgattt aacattttca tattttttaa ttgtgatgta
     8041 tgtagcatat aataggctgt ttgatataat aattgatttg acacattaaa tccactatat
     8101 ggatctttaa taaagttatc tacttcaatt ggattactag aaatccaatc actttgtgta
     8161 cgtaatggac gtattttttt attaaaacta tttgatacta actggttaac ccattcaact
     8221 cgtttctcag caccataaat ttttgtaatc agttgtaaca ctttaacggt tggtaaacct
     8281 ttccataatg aatacatacc agtaccactt aaaattagac catcaacata ttgcggatat
     8341 gtttctacaa acaatctagc tataacggat cccattgaat gtccgattat aatgtatggt
     8401 ttatcaacat tgcctctcac tgtttgcgct acttcaaagg catcaccgat aactcgtttc
     8461 atatcatcgt aatgccctct tgttgattca tcaatattaa taccatgtcc tcgatgatta
     8521 tgacgtatca catcgaagcc atgctttgaa agtgcatgtg ctaatttatc gtaacgttcc
     8581 atatgttcag ccataccatg aaatatatga ataattcctt ttgaatctac atctgtaaaa
     8641 ttcactttaa cttcaatatt cgtattatct tgtacagtta atttaaattg tttttcagtc
     8701 atcatctcac acctttaata acaacgcttt tatattcgct tttattatta tcatacattt
     8761 agtgatgcat agtttcaatt gactaatgta tctcgtataa aaaaactgat gcccttaaaa
     8821 caggacacca gtttatgatt gtacaaatta attattcttt aactgctggt tgattatttc
     8881 catgttgcgc ttctttaatt gcttgctcaa cttcttcagt atatgcttca atttgagctg
     8941 gagagtaatc aagcatatct gccataatat cgatgacagc atctttataa tctaatacat
     9001 ctttaatatt gaaatacatt ttaccagaac gacgaactaa gaaatcgtta ggtttgtata
     9061 ccatttcttg ttgaatacta taaacaagtt ctacataaat ttctaatggt aacttgctat
     9121 catggtattg agatgtttgc gcaatgttga acaattcatc aacatttgaa ccatatttag
     9181 atgctaaacg tcttgcaaca tcttcatcaa tgccgaatcc tttagctaca tctacttttt
     9241 gctctacaaa cgcatcaaag ttcttgctac cacctacatc gccacctgaa attgccagac
     9301 cttttgtatt acatggacta aatgttaaac cgtagtcttt tttcaagcgt ttagatacta
     9361 aatcaacaat gtcttgagcc atgtgacgat agcctgttaa tttaccacct gcaatagtta
     9421 ataaacctga tttaccttcc caaatttcat ccttacgaga gatttcagaa gggtctttgc
     9481 cttcttcgta aattaatggt ctaattcctg cccatgttga ttcaatatct tcatctgtaa
     9541 cattaacact agggaacatg taattaatcg catcgattaa atagtctctg tcttcttgtg
     9601 tagttaatgg tgaagatttg atattgtcat agaatgtatc tgtagtacct acatacgctt
     9661 ttccttcacg tggaattgca aaaatcattc ttccatcttt ttcagtatca aagtatactg
     9721 cttgacctaa tgggaatttt gattgatcaa taacaacatg tacaccttta gttaaacgta
     9781 attttttatt attgcgtgca taatcaccac ttctaacatc atcaacccat ggacctgctg
     9841 cattaaccac ttttttagcc ttaattgtat aattttcatt agttaattta tctataactt
     9901 taacaccatt tacttgttga tttttatcat aagtgaagtg ttcagattta gtatagttga
     9961 taatttctgc gcctttttca gcagcacgct tcataacttc aatagttaaa cgcgcatcgt
    10021 cagtacgata ttcaacatag taaccgccgc cttttagacc ttctttttta actaatggtt
    10081 ctttagctaa agtttctttt ttagataaca tttttttacg ttcagacttc ttaacacctg
    10141 ctaaacgatc atacattcct aaaccaattg atgttgagaa tttaccaaat gttccacctt
    10201 tatgcattgg taaaagcatc cactctggag tcgtaacatg aggcccattt tcataaacaa
    10261 tcgcacgttc tttaccagtt tcggcaacta ctccaatttg gaattgtttt aagtaacgca
    10321 aaccaccatg gactaatttt gtagatcttg agcttgttcc ttgtgcaaag tcttgcattt
    10381 caactaatgc aactttcatt cctctttcac tcgcgtctag tgcaatacct gcacctgtaa
    10441 taccgccacc aataattact aaatcatatt catcatttct taaattcttt ttaatatgtt
    10501 ctctcttaaa agtagacaat gccattccaa aacgcctcct aaaaaatgta aaaacgagag
    10561 acttgtccca cataaacaat ccttcaattg tacatgttcg aacaaatctc tcgcttctcg
    10621 attcaaatat taacttaaat tgattgtatc acaatgttta tacaaaagtc taatctacaa
    10681 gtttattctg ttttaaaaac ttgtgttgct tcaacagctt ttttccaacc tctatataat
    10741 ttttctcttt cgccttcatc cattttcgga tcgaattttt cttctaattt ccagttttta
    10801 gcgatatcat ctttactctc ccagaatcca actgctaaac ctgctaaaaa tgcagcacct
    10861 aaagctgtag tttcttgaat ttcaggtctt tcaacagaag tattaacaat gtctgcttgg
    10921 aactgcataa taaagttatt tttaactgca ccaccatcga cacgtaaact ttggacatca
    10981 ataccagagt cttttgacat tgcttccata acgtcacgag tttggtaaca tagtgattct
    11041 aaagttgcac ggataaagtg ctctttttca gttccacgtg ttaaaccgaa aatcgcacca
    11101 cgtgcttcag aatcccaata tggtgttcct aaacctacaa aagctggaac aacataaaca
    11161 ccctcagtag agtcaactcg tgtcgcataa ctttctgatt gtggtgctga attaatcatt
    11221 cttaatccat cacgtaacca ttggattgct gaacccgaaa caaagatgga accttcaagc
    11281 gcataattta cttttccatc aataccataa gcaattgttg ttaataaacc actttcagat
    11341 ttaaccgctt tgtcacctgt attcattaac atgaagccac cagttccata tgtgtttttc
    11401 acgtcaccac gttcgaagca agcttgtcca aataatgctg cttgttgatc accagctact
    11461 ccagcgattg gtacttcttg accatagaag tggtaatcaa ttgtcttacc atatacttca
    11521 ctcgaagctt taacttctgg caacatattt ttaggtactg taagtagttc taataactca
    11581 tcgtcccatt ctaaatcatg gatattaaac attaatgtac gactcgcatt tgaataatca
    11641 gtaatatgcg cagcttttcc tgataatttc catactaacc aagtatcaat cgttccaaat
    11701 aatagatcgc cattttctgc tttttctcgt gcaccttcaa cattgtctag aatccattta
    11761 acttttgtac ctgcaaaata cggatctaaa agtaatcctg tcttatctct aaatgtttgt
    11821 tcatatcctt gttgttttaa ttctgaacaa attgattgtg tttgacgtga ttgccaaaca
    11881 attgcgtgat aaattgggcg gccagtatgt ttgtcccaaa caaccgttgt ttcacgttgg
    11941 tttgtaatac cgatacctgc aatttgatca gctctaacat cattttcatt aattacttcc
    12001 gtcattacag ctaacacaga tgtccaaatt tcatttgcat catgttcaac ccaacctgat
    12061 tgtggaaaat attgcttaaa ctcacgttgt gctacccctg caatttcccc tttttgattg
    12121 aataaaatcg ctcttgagct tgttgttcct tggtctatag ataaaatata tttttccatt
    12181 agtagtatct cctttattaa aaaataatcg ctaacccatt gtacaaaatc tgttaaattt
    12241 tggcgaaaaa atagcataat ccatgaatca aacatgttaa attatgctat ttacgtttct
    12301 ttttatttta gtaaattgat tcgatatcac ctttttttga tgatttattt aaaatgtaac
    12361 ctaaaatcaa agtaataata actacaacaa ttgcaattgc acaaccaata ttaaatgtat
    12421 gtttataaaa tacagcgtat accactgcac ctaataaacc accggcaatt ggtcctaaga
    12481 taggaacgat tgcatatgac caatttgaac cacctttacc agctattggt aaaatcgcat
    12541 gtgcaattct cggacctaaa tcacgtgctg ggttgattgc ataaccagta gcaccgccta
    12601 aacttaatcc gattgcaaca attaatgctc cgacaattaa aggatttaaa ccatcggcaa
    12661 ttttgtttac accgataaat aaaataccta aagttaatgc cattgttccg ataatctcac
    12721 ttaaaaagtt ggcaaagtaa ttcttaatag ccggtgctgt agagaaaaca cctaatttcg
    12781 cgccagcttc ttctgtcgct ttccaatgtg gcaagtacat taaccataca attgttgctc
    12841 cgacaattgc acctaacatt tgagcaacaa tataaccagg aactaatgac caatcaaaac
    12901 ttccgtctaa tgcaagagct aaagacaccg ctgggtttaa atgtgcacct gagaattgac
    12961 cgacagcaaa cacacccatt gtaaccgcta atccccatcc agctgtgatg acaatccaat
    13021 cagcaccatt cgcagcactt ctctttaaat tgacattggc acaaacgcca ccaccaaaaa
    13081 ggattaagat tgcagttcct aggaattctg ctaaatatac attcatatta aatgtcctcc
    13141 caaaaagaca aagagattcc tacactagca gtttggctcg tagaaatctc ccgtctctat
    13201 tctatgtatt aacttttatt atatttgtta tgatactatc gatatgaaag cgttgtcaat
    13261 ggtttttgta aaaattttgt caaatt
//
LOCUS       DALAGD010000082        12920 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.083, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000082 DALAGD010000000
VERSION     DALAGD010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12920
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.083"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            56..187
                     /locus_tag="O2701_002060"
     CDS             56..187
                     /locus_tag="O2701_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293942.1"
                     /translation="MLLPSSKSHIEMMCEMFKLRSFCENKDNNYSFYNSEKSVYDNN"
     gene            449..1108
                     /gene="arlR"
                     /locus_tag="O2701_002061"
     CDS             449..1108
                     /gene="arlR"
                     /locus_tag="O2701_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor ArlR"
                     /protein_id="HCZ4293943.1"
                     /translation="MTQILIVEDEQNLARFLELELTHENYNVDTEYDGQDGLDKALSH
                     YYDLIILDLMLPSINGLEICRKIRQQQSTPIIIITAKSDTYDKVAGLDYGADDYIVKP
                     FDIEELLARIRAILRRQPQKDIIDVNGITIDKNAFKVTVNGAEIELTKTEYDLLYLLA
                     ENKNHVMQREQILNHVWGYNSEVETNVVDVYIRYLRNKLKPYDRDKMIETVRGVGYVI
                     R"
     gene            1105..2460
                     /gene="arlS"
                     /locus_tag="O2701_002062"
     CDS             1105..2460
                     /gene="arlS"
                     /locus_tag="O2701_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase ArlS"
                     /protein_id="HCZ4293944.1"
                     /translation="MTKRKLRNNWIIVTTMITFVTIFLFCLIIIFFLKDTLHNSELDD
                     AERSSSDINNLFHSKPVKDISALDLNASLGNFQEIIIYDEHNNKLFETSNDNTVRVEP
                     GYEHRYFDRVIKKRYKGIEYLIIKEPITTQDFKGYSLLIHSLENYDNIVKSLYIIALA
                     FGVIATIITATISYVFSTQITKPLVSLSNKMIEIRRDGFQNKLQLNTNYEEIDNLANT
                     FNEMMSQIEESFNQQRQFVEDASHELRTPLQIIQGHLNLIQRWGKKDPAVLEESLNIS
                     IEEMNRIIKLVEELLELTKGDVNDISSEAQTVHINDEIRSRIHSLKQLHPDYQFDTDL
                     TSKNLEIKMKPHQFEQLFLIFIDNAIKYDVKNKKIKVKTRLKNKQKIIEITDHGIGIP
                     EEDQDFIFDRFYRVDKSRSRSQGGNGLGLSIAQKIIQLNGGSIKIKSEINKGTTFKII
                     F"
     gene            2744..5542
                     /locus_tag="O2701_002063"
     CDS             2744..5542
                     /locus_tag="O2701_002063"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCZ4293945.1"
                     /translation="MTNERKEVSEAPVNFGANLGLMLDLYDDFLQDPSSVPEDLQVLF
                     STIKNDDSIVPALKSTSSQNSDGTIKRVMRLIDNIRQYGHLKADIYPVNPPKRKHVPK
                     LEIEDFDLDQQTLEGISAGIVSDHFADIYDNAYEAILRMEKRYKGPIAFEYTHINNNT
                     ERGWLKRRIETPYKVTLNNNEKRALFKQLAYVEGFEKYLHKNFVGAKRFSIEGVDALV
                     PMLQRTITIAAKEGIKNIQIGMAHRGRLNVLTHVLEKPYEMMISEFMHTDPMKFLPED
                     GSLQLTAGWTGDVKYHLGGIKTTDSYGTMQRIALANNPSHLEIVAPVVEGRTRAAQDD
                     TQRAGAPTTDHHKAMPIIIHGDAAYPGQGINFETMNLGNLKGYSTGGSLHIITNNRIG
                     FTTEPIDARSTTYSTDVAKGYDVPIFHVNADDVEATIEAIDIAMEFRKEFHKDVVIDL
                     VGYRRFGHNEMDEPSITNPVPYQNIRKHDSVEYVFGKKLVNEGVISEDEMHSFIEQVQ
                     KELRQAHDKINKADKMDNPDMEKPADLALPLQADEQSFTFDHLKEINDALLTYPDGFN
                     ILKKLNKVLEKRHEPFNKEDGLVDWAQAEQLAFATILQDGTPIRLTGQDSERGTFSHR
                     HAVLHDEQTGETYTPLHHVPDQKATFDIHNSPLSEAAVVGFEYGYNVENKKSFNIWEA
                     QYGDFANMSQMIFDNFLFSSRSKWGERSGLTLFLPHAYEGQGPEHSSARLERFLQLAA
                     ENNCTVVNLSSSSNYFHLLRAQAASLDSEQMRPLVVMSPKSLLRNKTVAKPIDEFTSG
                     GFEPILTESYQADKVTKVILATGKMFIDLKEALAKNPDESVLLVAIERLYPFPEEEIE
                     ALLAQLPNLEEVSWVQEEPKNQGAWLYVYPYVKVLVADKYDLSYHGRIQRAAPAEGDG
                     EIHKLVQNKIIENALKNN"
     gene            5556..6824
                     /gene="sucB"
                     /locus_tag="O2701_002064"
     CDS             5556..6824
                     /gene="sucB"
                     /locus_tag="O2701_002064"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCZ4293946.1"
                     /translation="MPEVKVPELAESITEGTIAEWLKNVGDSVEKGEAILELETDKVN
                     VEVVSEEAGVLSEQLASEGDTVEVGQAIAIIGEGSGNASKENSNDNTPQQNEETNNKK
                     EETTNNSVDKAEVNQANDDNQQRINATPSARRYARENGVNLAEVSPKTNDVVRKEDID
                     KKQQAPASTQTTQQASAKEEKKYNQYPTKPVIREKMSRRKKTAAKKLLEVSNNTAMLT
                     TFNEVDMTNVMELRKRKKEQFMKDHDGTKLGFMSFFTKASVAALKKYPEVNAEIDGDD
                     MITKQYYDIGVAVSTDDGLLVPFVRDCDKKNFAEIEAEIANLAVKAREKKLGLDDMVN
                     GSFTITNGGIFGSMMSTPIINGNQAAILGMHSIITRPIAIDQDTIENRPMMYIALSYD
                     HRIIDGKEAVGFLKTIKELIENPEDLLLES"
     gene            7404..8213
                     /locus_tag="O2701_002065"
     CDS             7404..8213
                     /locus_tag="O2701_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCZ4293947.1"
                     /translation="MCGLRSITLGTTNIEQTKHFMVDILGLNYEELLENSIRFGDADI
                     SPGTRLQFIQVPSEQLEESHFVGIGLRTPTDSGLEEYAEILSNKDIPFTTVKELNGNK
                     YFSLEDNNGHIFSIYSNENNYGVGLGMPSFESAVNPLHQVQGLGPVILKVNHVDITGQ
                     ILTNIFGLEVFAEYQPFDNADYHVQVFKVGTGGLGGEIHLMPVETEMTMPEYGAVDQV
                     EFETKDADFFNQAKSRLDEVEIPYQTLEQDDIESIRITENSGLSFIFTLQK"
     gene            8242..8445
                     /locus_tag="O2701_002066"
     CDS             8242..8445
                     /locus_tag="O2701_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293948.1"
                     /translation="MLHETWKERTPIKKVEVINTDAKKFTVSDMLTVGKQYDVINETE
                     EYYQIIDNSGLVGGYYKTYFKEV"
     gene            8626..9417
                     /locus_tag="O2701_002067"
     CDS             8626..9417
                     /locus_tag="O2701_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCZ4293949.1"
                     /translation="MALKHYKNSDSTVFNDAKALFDLNKNILLKGPTGSGKTKLAETL
                     SEVVDTPMHQVNCSVDLDTESLLGFKTIKTNAEGQQEIVFVDGPVIKAMKEGHILYID
                     EINMAKPETLPVLNGVLDYRRQITNPYTGEVIKAVPGFNVIAAINEGYVGTLPMNEAL
                     KNRFVVIHVDYIDGDILKNVIKEQSLLQGDKQIEQIIKFNEDLRTMSKQGQISEEAAS
                     IRALLDLCDLITVMPVERAIKRTIIDKLEDEREQQAIYNAVELNF"
     gene            9431..11317
                     /locus_tag="O2701_002068"
     CDS             9431..11317
                     /locus_tag="O2701_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation protein NorD"
                     /protein_id="HCZ4293950.1"
                     /translation="MSDRFIKFNDEQLDAKQVMMLQDLARLLLKNEQTQVKIQKFPYY
                     NPVQNVLITSWFWSHRPSHIEMAGLKTDVMLAAYGYHMMDVQIVNEVVQDKTFKHPKF
                     YQQLFKLLEDMRVLNSIKVERPSTAKLIDLRLDTRISYTESQIKVYRTKTQYTDLLFL
                     YLEHAFLSQDFFDIPSIHSDLDDILVNMFLYLPNFFQNQNSEDNMYLAQRIMYQVDDI
                     LKEDMLNEYYYLPKTLYNTLASPEFDDLKRTDASQVDGQDDTSEDDDNESEKADSKSA
                     DSESKGGAYLEMELHEGQNSETLGNDEAREGDATDDMTDMMTKKGKGSNDTLNREEGD
                     AVGQSQAFQLDGVNKNVEIKWQIPEIEPQYVLEYQESKQDVQYEIKDLIQIIKKTIER
                     EQRDARFNLTKGRLQKDLINWFIDDQYKLFYKKQDLSKSFDATFTLLIDASASMHDKM
                     AETKKGVVLFHETLKALNIKHEILSFSEDAFDSDEHAQPNIINEIINYDYSTFEKDGP
                     RIMALEPQDDNRDGVAIRVASERLMRRNQHQRFLIVFSDGEPSAFNYSQDGIIDTYEA
                     VEMSRKFGIEVFNVFLSQDPITEDVEQTIHNIYGQYAIFVEGVAHLPGHLSPLLKKLL
                     LKSL"
     gene            11542..12885
                     /gene="brnQ"
                     /locus_tag="O2701_002069"
     CDS             11542..12885
                     /gene="brnQ"
                     /locus_tag="O2701_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCZ4293951.1"
                     /translation="MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFV
                     LTGIGLPLLGVIVGALDKEGYIGALNKISPKFSILFLIIIYLTIGPLFAIPRTASTSF
                     EMTITPIIHSNSSIALFIFTIIYFIVVLYICLNPSKLIDRIGSLLTPLLLITILAMII
                     KGYLDFSGNSAGKGNEALYHSNFSSFAEGFTQGYLTMDAIAAIAFSMIVVNAVKLTGI
                     TKTNQIFKQTLTAGLIAAVALIFIYISLGYIGNHMPVSDMTLDQLKSKDRNIGTYLLT
                     TMASTGFGSFGKYLLGIIVALACLTTACGLIVAVSEYFHRIVPKVSYKAFVLVFILMS
                     FIIANQGLNAVISMSIPVLSIVYPVAITVVLLILIAKFIPTKRISQQIPVIIVFILSI
                     FSVISKLGWLKINFIESLPLRAYSLEWFPVAIIATILGYLVGIFVKQDPIKYQQE"
BASE COUNT         4783 a         1844 c         2288 g         4005 t
ORIGIN      
        1 tgaggtttaa acattatatt gtttacatac tccttttgaa ttcatacatt atgaaatgtt
       61 acttccaagt tcaaaatcgc acattgaaat gatgtgtgaa atgtttaaac tacggtcatt
      121 ttgtgaaaat aaagataata actattcatt ttacaatagt gaaaagtcag tatatgacaa
      181 caattaatat tgcggtaagg ccttgtgtta cagtattcta tatttaagta ctgcaatcag
      241 aattaacaga atgccattaa ctgattatta aatatttgag ttaataaata attaatgatt
      301 gtagcttgaa aaatttaaaa catggttatt gatttgtgat aaaatttaaa cgtaaacaaa
      361 ctaatttaaa aagcaactat tgtatagaaa aatacaaaat ttaaaatata ttaccttatt
      421 agaaaaagtc gtaatatgag gtgtacaaat gacgcaaatt ttaatagtag aagatgaaca
      481 aaacttagca agatttcttg aattggaact cacacatgaa aattacaatg tggacacaga
      541 gtatgatgga caagacggtt tagataaagc gcttagccat tactatgatt taatcatatt
      601 agatttaatg ttgccgtcaa ttaatggctt agaaatttgt cgcaaaatta gacaacaaca
      661 atctacacct atcattataa ttacagcgaa aagtgatacg tatgacaaag ttgctgggct
      721 tgattacggt gcagacgatt atatagttaa gccgtttgat attgaagaac ttttagcaag
      781 aattcgtgca attttacgtc gtcagccaca aaaggatatt atcgatgtca acggtattac
      841 aattgataag aacgctttta aagtgacggt aaatggcgca gaaattgaat taacaaaaac
      901 agagtatgat ttactatatc ttctagctga aaataaaaac catgttatgc aacgggaaca
      961 aattttaaat catgtatggg gttataatag tgaagtagaa acaaatgtcg tagatgttta
     1021 tataagatat ttacgaaaca agttaaaacc atacgatcgt gacaaaatga ttgaaacagt
     1081 tcgtggcgtt gggtatgtga tacgatgaca aaacgtaaat tgcgcaataa ctggattatt
     1141 gttaccacga tgattacgtt tgtcacgata tttttgtttt gtttaattat tatttttttc
     1201 ttgaaagata cactgcataa tagtgagctt gatgatgcag aacgaagctc aagcgatatt
     1261 aataatttat ttcattctaa gcctgttaaa gatatatctg cattagactt gaatgcatct
     1321 ttaggtaatt ttcaagagat aattatttat gatgagcata ataataaatt atttgagaca
     1381 tcgaatgata acacagtgag agttgaacca ggttatgaac accgttattt tgaccgcgta
     1441 ataaaaaaac gctataaagg cattgaatat ttaattatta aagaaccaat tacaacgcaa
     1501 gatttcaaag ggtatagctt gttaattcat tcactagaaa attatgataa catcgtaaaa
     1561 tcattgtata tcattgcgct ggcatttgga gtgattgcaa caattataac tgccacaatc
     1621 agttatgtat tttcaacaca aattactaaa ccgcttgtca gtttatcaaa taaaatgatt
     1681 gagattcgac gagatggttt tcaaaataaa ttgcaattaa atacaaatta tgaagaaata
     1741 gataatttag caaatacgtt taatgagatg atgagccaaa ttgaagaatc atttaatcaa
     1801 caaagacaat ttgttgaaga tgcgtcacat gaattacgaa caccattaca aattattcaa
     1861 ggtcatttaa atttgattca gcgatgggga aaaaaagacc cagcagtatt agaagaatcg
     1921 ttaaatattt ctattgaaga aatgaatcgt atcataaaat tagtcgaaga attacttgaa
     1981 ttgactaaag gagatgtaaa tgacatttct tctgaagcac agaccgtgca tattaatgat
     2041 gaaattcgct cgcgaataca ctcattaaaa caattgcatc ctgattatca atttgatacg
     2101 gatctgacat ctaaaaatct agaaattaaa atgaaacctc atcaattcga acaattattt
     2161 ttaatcttta ttgataatgc aatcaaatat gatgtgaaga ataagaaaat taaagttaag
     2221 acaaggttaa aaaataagca aaaaataatt gaaattacag atcatggaat tggtattcca
     2281 gaggaagatc aagatttcat ttttgatcgc ttttatcgag tggataaatc tcgttcaaga
     2341 agtcaaggcg gtaatggact cggattatct attgctcaaa aaatcattca attaaacgga
     2401 ggatcgatta aaattaaaag tgaaattaac aaaggaacaa cgtttaaaat catattttaa
     2461 tcatgactga gacgtcaatc aaagtcatag gatcaatttt ttaagtacac attagctgtg
     2521 actaatgtat aagaacaact ataaaacaaa taaacagtgg ttctttatca tttctgttgt
     2581 actcccaaaa tttacaataa aatacatcta taaacctaga agaatcaacg cttttgttga
     2641 ttcttctttt tagcagataa ataggtaaat ctactttaac aaataactaa atagtgatat
     2701 tattacattg taagcgtttc aacatttttg tggagggtgt aaaatgacta acgaaagaaa
     2761 agaagtttca gaggctcctg taaacttcgg tgcgaattta ggtctaatgt tagatctata
     2821 tgatgacttt ttacaagatc catcatctgt accagaagat ttacaagtct tattcagcac
     2881 aattaagaat gatgactcaa ttgtaccagc tttaaaaagt acaagtagtc aaaatagcga
     2941 cggcacaatt aagcgtgtca tgcgtttaat tgataatatt cgccaatacg ggcatcttaa
     3001 agccgatatt tatcctgtaa atcctccaaa aaggaaacat gtacctaaat tagagattga
     3061 agactttgat ttagatcaac agactttgga aggtatatca gcaggaattg tttcagatca
     3121 ctttgccgac atttatgata atgcttatga agcaatttta agaatggaaa aacgttacaa
     3181 aggaccaatt gcatttgagt atacacatat taataacaat accgaacgtg gttggttaaa
     3241 aagaagaatt gaaacgccat ataaagtaac gttaaataat aacgaaaaaa gggcactatt
     3301 caaacaatta gcgtatgttg aagggtttga aaaatatctt cataaaaact tcgttggtgc
     3361 aaagcgtttt tcaattgaag gggtagacgc acttgtaccg atgttacaac gtactattac
     3421 gattgctgcg aaagaaggta ttaaaaatat acaaataggc atggctcacc gtggacgttt
     3481 aaacgtttta acgcatgtct tagaaaaacc gtacgaaatg atgatttcag aatttatgca
     3541 tacagatcca atgaaattct tacctgaaga tggtagcttg cagttaactg ctggatggac
     3601 tggtgatgtg aaatatcacc ttggtggcat taaaactact gattcatacg gtacaatgca
     3661 gcgtattgca ctggctaaca atccaagtca cttggaaatt gttgcacctg ttgttgaggg
     3721 gcgtacgaga gcagcacaag atgatacaca acgagctggg gctccgacga ctgatcatca
     3781 taaagcaatg ccaattatta tacatggcga tgctgcttat cctggtcaag gaattaactt
     3841 cgaaacaatg aacttaggaa acttgaaagg ctattctacg ggtggttcat tgcatattat
     3901 tactaacaat agaattggat ttactacaga accaattgat gcacgttcaa caacttattc
     3961 tacagatgtg gccaaaggtt atgatgtgcc aatattccat gtcaatgcag atgacgttga
     4021 agctactatt gaagcaattg atattgcaat ggaatttaga aaagagtttc ataaagacgt
     4081 cgttattgat ttagtaggtt atcgtcgttt cggacataac gaaatggatg aaccatcaat
     4141 tactaatcca gttccttatc agaatattcg caaacatgac tctgttgaat atgtgtttgg
     4201 taaaaagctt gttaatgaag gtgtcatttc agaagatgaa atgcattcat ttatagaaca
     4261 agtccaaaag gaactaagac aagctcatga taaaattaat aaagctgata aaatggataa
     4321 tccagatatg gaaaagcctg cagatcttgc attaccgtta caagcagacg aacaatcatt
     4381 tacttttgat cacttgaaag aaataaatga tgcattgtta acatatccgg atggctttaa
     4441 cattttgaaa aagttaaaca aagttcttga gaagcgtcat gagccgttta ataaagaaga
     4501 tggtttagtt gattgggcac aagcagaaca acttgcattt gcgacaattt tacaagatgg
     4561 tacaccgatt cgcttaactg gtcaagatag tgaacgtggt acattcagtc ataggcatgc
     4621 cgtgttacat gatgagcaaa caggtgaaac atatacacct ttacatcatg ttcctgatca
     4681 aaaagcgaca tttgatatac acaattctcc gctttcagaa gcagcagtag ttggttttga
     4741 atacggctat aatgtggaaa acaaaaaaag cttcaatatt tgggaagcac aatatggtga
     4801 ttttgcaaat atgtcacaaa tgatttttga caacttctta ttcagttctc gctcaaaatg
     4861 gggagaacgt tcaggattaa cattattctt acctcatgca tatgagggtc aagggcctga
     4921 acattcatca gcaagattag agcgattttt acaattagct gctgaaaata attgcacagt
     4981 tgtcaactta tctagttcaa gtaattattt ccacttattg cgtgcacaag cggctagttt
     5041 agattctgaa caaatgcgac cattggttgt tatgtcacca aaaagcttac tgagaaataa
     5101 aacagttgca aaaccaattg atgaatttac ttctggtgga tttgagccaa ttttgacaga
     5161 atcatatcaa gcggataagg ttacaaaagt tattttggca actggtaaaa tgttcattga
     5221 tttaaaagaa gcattagcta aaaatccaga cgaatcagta ttactcgttg cgattgaaag
     5281 attgtatcca ttcccagagg aagagattga agcattacta gcacaattgc caaaccttga
     5341 agaagtgtca tgggtacaag aagaacctaa aaatcaaggt gcatggttat atgtctatcc
     5401 atatgttaaa gtgctagttg cagataaata tgatttaagt tatcatggca gaattcaaag
     5461 ggctgctcca gctgaaggcg atggagaaat tcataaactt gttcaaaata aaattataga
     5521 aaatgcatta aaaaataact agggggaaat aagtcatgcc agaggttaaa gttccagaat
     5581 tagcagaatc tattacagaa ggtaccattg cagaatggtt gaaaaacgta ggggatagcg
     5641 tagaaaaagg tgaagctatt cttgaattag aaactgataa agttaatgtc gaagttgtat
     5701 ctgaagaagc aggtgtatta tctgaacaac ttgcaagtga aggcgacact gtagaagttg
     5761 gacaagcaat tgctatcatc ggcgaaggta gtggcaatgc ttctaaagaa aatagtaacg
     5821 acaatactcc acaacaaaat gaagaaacaa ataataaaaa agaagaaaca acaaataatt
     5881 cggtagataa agctgaagta aatcaagcaa atgatgacaa tcagcaacgt attaatgcta
     5941 cgccttctgc gcgtcgatat gctcgtgaaa atggtgtgaa tcttgctgaa gtaagtccga
     6001 aaacaaatga tgtggttcgt aaagaagata ttgataagaa acaacaggca ccggcatcaa
     6061 cacaaacaac acaacaagca tctgcaaaag aagagaaaaa atacaatcaa tatcctacaa
     6121 aaccagtgat tcgtgaaaaa atgtcacgta gaaagaaaac agctgccaaa aaattattag
     6181 aggtatctaa taatacagct atgttaacaa catttaacga agttgacatg acaaatgtta
     6241 tggaattgcg taaacgtaag aaagaacaat ttatgaaaga tcatgatggt actaaattag
     6301 gatttatgtc attctttact aaagcttctg tagcagcttt gaaaaagtat ccagaagtta
     6361 atgcagaaat cgacggcgac gacatgatta cgaaacaata ttatgatatt ggtgtagctg
     6421 tttctacaga tgatggatta ttagtaccat ttgtaagaga ttgtgataaa aagaattttg
     6481 cagaaatcga agcagaaatt gctaatttag cagttaaagc acgagagaaa aaacttggct
     6541 tagatgatat ggttaatggt tcatttacga ttacaaatgg cggtattttt ggatcaatga
     6601 tgagtacgcc aattatcaat ggtaatcaag ctgcaatctt aggcatgcat tcaattatta
     6661 caagaccaat tgcgattgat caagatacaa tcgaaaatcg tccaatgatg tatattgcat
     6721 taagctatga tcatagaatt attgacggta aagaagcagt tggattctta aaaacaatta
     6781 aagaattaat tgaaaaccca gaagacttat tattagaatc ttaatccaca acacaaaata
     6841 gtttactatt cctaaaagcg ggattaaatc aataacaaac agcagtaaga ttatttccta
     6901 gtcgaaatat cttactgctg tactttattt ttataatgat tcgtattaaa atgtgggttt
     6961 aattttgact acttaaaaaa tatatcattt ctattgaaat agactcacaa tagaaatata
     7021 ggtagaatag cgtgtttcaa tatgctaatg aatgcatttt agataataac aaacgatgaa
     7081 atatatgaat tctataagcg cctttagtta atttaaaatc atctgaacat gatgtaaatt
     7141 cgctttatgg acacgacata tatatgtttt tcatgtccta caaaaaacga aatattcaat
     7201 tgctatatac agatattcat taatgacaca ctcaatagca accataacaa tattatgtta
     7261 cctatttaaa tgaaatagtt cttttaaaga aatacatttt tcacatatta atctataatc
     7321 aaaatcaact gaccgatatt ctataattta tgattaaaat aagttataat ataatagtaa
     7381 agataaagat agaggtggct ataatgtgtg gacttagaag tataacatta ggtacaacaa
     7441 atatagaaca gacaaaacat ttcatggttg acatattagg attaaattat gaagaacttc
     7501 ttgaaaactc aattcgtttc ggcgatgcag atataagccc aggaacaaga cttcaattta
     7561 tacaagttcc aagtgagcaa ttagaagaat ctcactttgt gggtattgga ttacgtacac
     7621 caactgactc aggtttagag gagtatgcgg aaatattatc gaataaggat attccattta
     7681 caacagttaa agaattaaat ggcaataaat atttcagtct cgaagataac aatggtcata
     7741 ttttctcaat atattcaaac gagaataatt atggcgttgg tttaggtatg ccttcttttg
     7801 agagtgcggt caatccgtta catcaagtgc aaggtttagg accagtgatt cttaaagtga
     7861 atcatgtaga tattacaggt caaattttaa caaatatatt cggacttgaa gtatttgcag
     7921 aataccaacc cttcgacaat gctgactatc atgtccaagt attcaaagtt ggaacgggtg
     7981 gtctaggtgg cgaaatacat ttaatgcctg tagaaaccga aatgacaatg ccggaatatg
     8041 gcgcagtcga tcaagttgag tttgaaacga aagatgcaga tttctttaat caagcgaaat
     8101 cgcgcttaga tgaagtggaa ataccatatc aaacgcttga gcaagatgat attgaatcaa
     8161 ttagaattac tgaaaacagt ggattatcgt ttatattcac tttacaaaaa taattttttt
     8221 acgatagcga ggacaaaatt tatgttacat gaaacttgga aagaacgtac accaatcaag
     8281 aaagtagaag tcattaatac agatgcaaag aaatttacag tttctgacat gttaacggtt
     8341 ggcaaacaat atgatgttat caacgaaact gaagaatatt atcaaataat agataattct
     8401 ggattagttg gcggttacta taaaacatat ttcaaagaag tttaaacgca taattattga
     8461 aatgaagcct aatatgagat tttcaattag taaatttggt aaaatataaa caattcgcat
     8521 actaagcatt gcgttttata tcagaataga atagtaaact aaggcatttg gagaacttat
     8581 tccatgtctt agttttttaa tttatttaaa ggagaacgtt ttaaaatggc actaaaacat
     8641 tataagaatt cagattcaac agttttcaat gatgcgaagg cattatttga tttaaataaa
     8701 aatattttac ttaaaggtcc aacaggttca gggaaaacaa agttggcaga aacattaagt
     8761 gaagttgttg atacacccat gcatcaagtc aattgttctg ttgatttaga tacagaaagc
     8821 ttattaggct ttaaaacaat taaaacaaat gcggaaggtc aacaagaaat tgtctttgta
     8881 gatggtccag ttattaaagc tatgaaagag gggcatattt tatatattga tgaaataaat
     8941 atggctaaac ctgaaacatt gcctgtatta aatggggtct tagattatcg tcgtcaaatt
     9001 acgaatccat acactggtga agtaatcaaa gctgtaccag gatttaacgt tatagcagcg
     9061 ataaatgaag gttatgttgg tactttgcca atgaatgaag cactaaaaaa tcgctttgtt
     9121 gttattcacg ttgattatat tgatggggac attttaaaaa atgtgattaa agagcaaagt
     9181 ttattacaag gtgataaaca aatcgaacaa attattaagt ttaatgaaga tttacgtact
     9241 atgtctaagc agggacaaat ttctgaagaa gccgctagta tccgtgcatt attagacttg
     9301 tgtgatttaa tcactgtaat gccagttgaa cgtgcaatta aacgtacaat tattgataaa
     9361 ttggaagatg aacgtgaaca acaagcaata tataatgctg tagaactaaa cttttaaaga
     9421 gggataaaac atgagtgatc gtttcataaa atttaatgac gaacagttag atgccaagca
     9481 agtaatgatg ttgcaagact tagctcgact cttattaaaa aatgaacaaa cacaagttaa
     9541 aatccaaaaa ttcccttact ataatcctgt tcaaaatgtt ttgattacga gttggttttg
     9601 gtcacatcga ccaagccata tcgaaatggc tggtttaaaa acagatgtta tgttagctgc
     9661 ttacggttat catatgatgg atgtacaaat tgtaaatgaa gtagtacaag ataaaacttt
     9721 taaacaccct aaattttatc aacaactttt taaattatta gaagatatgc gcgtattaaa
     9781 ttctattaaa gttgagcgcc caagtactgc taaattaatt gatcttcgct tagatacacg
     9841 tatatcatat acggaatctc aaattaaagt gtatcgtact aaaacacaat atacagattt
     9901 attatttttg tatttagaac atgcattttt aagccaagat ttctttgata taccatctat
     9961 acattcagat ttagatgata ttttagtaaa tatgttctta tatttaccta attttttcca
    10021 aaatcaaaat tctgaagata atatgtatct ggctcaaaga attatgtatc aagtagatga
    10081 tattttaaaa gaagatatgc taaacgaata ttattactta cctaaaacac tatataatac
    10141 attagctagt ccagagtttg atgatttaaa gagaactgat gcaagtcaag tcgatgggca
    10201 agatgatact tcagaagatg atgataacga aagtgaaaaa gctgactcta aatctgcaga
    10261 tagtgagtca aaaggtggcg cctatttaga aatggagctt cacgaaggac aaaatagtga
    10321 aacattaggt aatgatgaag cacgtgaagg cgatgctaca gatgatatga ctgatatgat
    10381 gaccaaaaag ggcaaaggat caaacgatac gctaaatcgt gaagaaggcg atgctgttgg
    10441 tcaaagccaa gcatttcaac ttgatggtgt gaacaaaaat gtagaaataa aatggcaaat
    10501 tcctgaaatt gagccacaat atgttttaga atatcaagaa tcgaaacaag acgtacaata
    10561 tgaaatcaaa gacttaattc aaattataaa gaaaacgatt gaacgagaac aacgagatgc
    10621 acgttttaat ttaacaaaag ggcgattaca aaaggattta attaattggt ttatcgacga
    10681 tcaatataaa ttgttttaca aaaaacaaga tttaagtaaa tcatttgatg cgacatttac
    10741 attgttaatt gatgcgtcag caagtatgca tgataaaatg gctgaaacga agaaaggtgt
    10801 agtgttattc cacgagacgt taaaagcatt gaacattaaa catgaaattt tatcattcag
    10861 tgaggatgcc tttgattcag acgagcatgc acaaccaaat atcattaatg aaattattaa
    10921 ctatgattac tcaacctttg aaaaagatgg cccacgtatt atggcacttg aacctcaaga
    10981 tgataatcgt gatggtgttg ctattagagt tgcaagtgaa cgattaatgc gtcgaaatca
    11041 acatcaacga tttttaatcg tattttcaga cggcgaaccg tctgcattta attatagtca
    11101 agatggtatt attgatacgt atgaagctgt agaaatgtca cgtaaatttg gtattgaagt
    11161 atttaatgta tttttgagtc aagatccaat aactgaagat gttgaacaaa caattcataa
    11221 tatttatggt caatatgcta ttttcgttga aggtgttgcg catttacctg gacatctctc
    11281 tccattatta aaaaaattac tacttaaatc tttataaata aatttaaaat atgagtttat
    11341 atgaaattgt gaataaaagt catcagttac tatttaaaaa gtggatttga agaattttat
    11401 tttctgacat aatttgattt aaacaacaca tgtgttttgt ttctgctatg tacaacatat
    11461 ttcatataga ctctttatta ttttttgtgc taaaatattt acttgtaaat tatttcgtgt
    11521 tttaaaaaaa ggagtaactc tatgaataaa aatacatggg tcattgggtt tacgctcttc
    11581 gctatgtttt ttggcgcagg caatctaatt tttcctccta atctcggatt ggatagtggt
    11641 caatttttct ggcccgccat tttagcgttt gttctaactg ggattggttt accattatta
    11701 ggtgtgattg taggtgcact tgataaagaa ggatatattg gcgcattaaa taaaatttca
    11761 cctaaatttt caatattgtt cttaatcatc atttatttga ctataggacc actttttgca
    11821 atacctagaa ctgcatctac atcttttgaa atgacaatta caccaattat acatagcaat
    11881 agtagtatcg ctttatttat atttacgatt atctacttca tagtcgtttt gtatatttgt
    11941 ttaaatccat ctaagttaat cgatcgtatt ggttcattat taacaccatt attattgatt
    12001 actattttag cgatgattat taaaggatac ttagacttta gcggtaatag tgctggaaag
    12061 ggcaatgaag cactatatca ttctaatttt tcaagttttg ctgaaggctt tacacaaggc
    12121 tatttaacaa tggatgccat tgcagcaatt gctttttcaa tgattgttgt taatgcagta
    12181 aaactaacag gcattactaa aacaaatcaa atattcaaac aaactttgac tgctggttta
    12241 attgcagccg tagctttaat tttcatatat atttcattag gttatattgg taatcatatg
    12301 ccagtaagtg acatgacgtt agatcaattg aaatccaaag atcgaaacat tgggacatat
    12361 ttattaacga caatggcttc aacaggattt ggttcattcg gaaaatattt attgggcatc
    12421 attgtggcgc tggcatgtct aactacagca tgcgggctta ttgttgcagt ttctgaatat
    12481 ttccatagaa tcgtacctaa agtatcatac aaagcatttg tattagtttt cattttaatg
    12541 agttttatta ttgctaacca aggtttaaat gctgttatct caatgtcaat tccggtatta
    12601 agcattgtat acccagtagc aataactgtt gtattattaa ttttaattgc caaattcata
    12661 ccgacaaaac gcatttcaca acaaattcca gttattatcg tatttatatt gtcgattttc
    12721 agtgttatta gtaagttagg ttggctgaaa attaacttta tagaatcatt gcctctaaga
    12781 gcgtattctt tagagtggtt cccagtagca attattgcaa cgatattagg ctatctagtc
    12841 ggcatatttg taaaacaaga tccaattaaa tatcaacagg aataacgaat aatataaaag
    12901 aggttgggac ataaatccct
//
LOCUS       DALAGD010000083        12768 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.084, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000083 DALAGD010000000
VERSION     DALAGD010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12768
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.084"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            101..1855
                     /gene="eap"
                     /locus_tag="O2701_002070"
                     /gene_synonym="map"
     CDS             101..1855
                     /gene="eap"
                     /locus_tag="O2701_002070"
                     /gene_synonym="map"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular adherence protein Eap/Map"
                     /protein_id="HCZ4293952.1"
                     /translation="MKFKSLITTTLALGVLASTGANFNNNEASAAAKPLDKSSSSLHH
                     GYSKVHVPYAITVNGTSQNILSSLTFNKNQNISYKDLEDRVKSVLKSDRGISDIDLRL
                     SKQAKYTVYFKNGTKKVIDLKAGIYTADLINTSEIKAININVDTKKQVEDKKKDKANY
                     QVPYTITVNGTSQNILSNLTFNKNQNISYKDLEDKVKSVLESNRGITDVDLRLSKQAK
                     YTVNFKNGTKKVIDLKSGIYTANLINSSDIKSININVDTKKHIENKAKRNYQVPYSIN
                     LNGTSTNILSNLSFSNKPWTNYKNLTSQIKSVLKHDRGISEQDLKYAKKAYYTVYFKN
                     GGKRILQLNSKNYTANLVHAKDVKRIEITVKTGTKAKADRYVPYTIAVNGTSTPILSD
                     LKFTGDPRVGYKDISKKVKSVLKHDRGIGERELKYAKKATYTVHFKNGTKKVININSN
                     ISQLNLLYVQDIKKIDIDVKTGTKAKADSYVPYTIAVNGTSTPILSKLKISNKQLISY
                     KYLNDKVKSVLKSERGISDLDLKFAKQAKYTVYFKNGKKQVVNLKSDIFTPNLFSAKD
                     IKKIDIDVKQYTKSKKNK"
     gene            1842..2156
                     /locus_tag="O2701_002071"
                     /pseudo
     CDS             1842..2156
                     /locus_tag="O2701_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669703.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
     gene            complement(2480..3766)
                     /locus_tag="O2701_002072"
     CDS             complement(2480..3766)
                     /locus_tag="O2701_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCZ4293953.1"
                     /translation="MNPLAQSLNEQLQQSNATAFTMLSDLGQNMFYPKGILSQSAEAK
                     STTYNATIGMATNKDGKMFASSLDAMFNDLTPDEIFPYAPPQGIEELRDLWQQKMLRD
                     NPELSIDNMSLPIVTNALTHGLSLVGDLFVNQGDTILLPEHNWGNYKLVFNTRNGANL
                     QTYPIFDKDGHYTTDSLVEALQSYNKDKVIMILNYPNNPTGYTPTHKEVTTIVDAIKA
                     LADKGTKVIAVVDDAYYGLFYEDVYTQSLFTALSNLNSNAILPVRLDGATKEFFAWGF
                     RVGFMTFGTSDQTTKEVLEAKVKGLIRSNISSGPLPTQSAVKHVLKNNKQFDKEIEQN
                     IQTLKERYEVTKEVVYADQYHSHWQAYDFNSGYFMAIKVHDVDPEELRKHLIEKYSIG
                     VIALNATDIRIAFSCVEKEDIPHVFDSIAKAIDDLR"
     gene            3966..4064
                     /locus_tag="O2701_002073"
     CDS             3966..4064
                     /locus_tag="O2701_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293954.1"
                     /translation="MMWWQEGMMTLITGGLLIIFRLWLELKWKNKK"
     gene            4307..4483
                     /locus_tag="O2701_002074"
     CDS             4307..4483
                     /locus_tag="O2701_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293955.1"
                     /translation="MKHLTKIFVVLAIILFIIGYYLQATNHESQGIKLLLAAIMFMIC
                     AFISRSNDRRKNSK"
     gene            4747..5127
                     /locus_tag="O2701_002075"
     CDS             4747..5127
                     /locus_tag="O2701_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCZ4293956.1"
                     /translation="MKIILKNNSDFPIYEQIKQQVKQNILKGHVAPGEHLPSMRELAK
                     DLQVSLITTKRAYEDLEKDGFVTTIRGKGTFVKEQDSSILKEKQFFTIENLVKELVNE
                     AQAIEMSLEELQDILTFIYEEESS"
     gene            5124..6020
                     /gene="pmtA"
                     /locus_tag="O2701_002076"
     CDS             5124..6020
                     /gene="pmtA"
                     /locus_tag="O2701_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtA"
                     /protein_id="HCZ4293957.1"
                     /translation="MNAIELSNVNYSSDQFNLKNISFKVPQGFVTGFIGRNGAGKTTI
                     IRLIMDLYQPQTGVIRVLEEDMALNPIELKNRIGFVYSENYFNERWTTKQLEKMIAPF
                     YRKWDHQVFEFYLEKFDLPINKSIKTFSTGMKMKLSLAVAFSHHAELYIFDEPTSGLD
                     PLARNELLEIIQQELIDENKTIFMSTHIISDLEKIADYIIHLSDGEVILNGSKEQLLQ
                     RYQVVSGAIEDLDDELASLLIYEEHKRTGFIGLTEHAQVFKEILGHKVNITTPSIENL
                     MVYLEKRKPKYHENIKLMEEGF"
     gene            6021..6701
                     /gene="pmtB"
                     /locus_tag="O2701_002077"
     CDS             6021..6701
                     /gene="pmtB"
                     /locus_tag="O2701_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtB"
                     /protein_id="HCZ4293958.1"
                     /translation="MKALLIRNFKLRRYTLIIYVLLLTLYPFYIMLDSTKFFYLLQSF
                     ISPTILIIWILDAGHLFRLNRRLGGNDSYYFYMSLPVSKKQLLNANYITCIVLTLIGT
                     LVISLYAYEADVIEPNSIYFSTAYAFVISNFLSIPIAFSQFTELRRVKVPYGIYVFTI
                     IILVPFLFSIAIVLVNYFVLSQSSFPDLYSYILNIGFLIISIVILIVNYFKQLNKINT
                     RKFKGGSR"
     gene            6698..7570
                     /gene="pmtC"
                     /locus_tag="O2701_002078"
     CDS             6698..7570
                     /gene="pmtC"
                     /locus_tag="O2701_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtC"
                     /protein_id="HCZ4293959.1"
                     /translation="MKLEHITKKYGSNVVLNDIDFDFGDSRIVGLIGKNGVGKTTVMK
                     VMNGNIIKFDGKVDIDNADNIGFLIEHPKLYDNKSGLYNLKLFAQVLGKGFDKAYTDK
                     IIDAFGMRPYIKKKVKKYSMGMKQKLAIAVSLMNKPKFLILDEPTNGMDPDGSIDVLT
                     TIKSLVNELDMRILISSHKLEDIELICDRAVFLRDGHFVQDVNMEEGVASDTTIVTVD
                     HKDFDRTEKYLAEHFQLQNVDKADGHLMINAQKNYQVILKALSELDIYPKYIETRKSS
                     LRDTYFNINQRGDK"
     gene            7570..8310
                     /gene="pmtD"
                     /locus_tag="O2701_002079"
     CDS             7570..8310
                     /gene="pmtD"
                     /locus_tag="O2701_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtD"
                     /protein_id="HCZ4293960.1"
                     /translation="MRILNLVKYDFYSIFKSPLTYLAILVVSSLIATQSILMANSMDN
                     PKHIIVYGSVFAAAKWLLLIIGLMFVVKTITRDFSQGTIQLYMSKVKTRVGYIISKTI
                     SIILISILFALIHYVILIVVQASSNGKNLAFSKYVDNLWFFLIFLLFFGLFLFLITLA
                     SQKTAMIFSLGVFLVLIVPFIKPFITFIPRYGEKVLDAFDYIPFAYLTDKMISSNFDF
                     SNWQWVISLGSIVIFFILNILYVAKKDI"
     gene            complement(8376..8939)
                     /locus_tag="O2701_002080"
     CDS             complement(8376..8939)
                     /locus_tag="O2701_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCZ4293961.1"
                     /translation="MTNLETYFKNSQPLNEYIDGMKTNQDTVLSIYQSFELPEDDSRI
                     KKIKEMNYSKVLVITEDWCGDAMMNLPILKHISEALNLEVRVFHRDDDTKLIDQYLTN
                     GKSRAIPIFVFLNDQFEQETVWGPRANEVQKFVTDVRADKLPSKDHPDYADLEKETHL
                     VISNRYKTDASFWKAVYNSILNKLENK"
     gene            9060..9584
                     /locus_tag="O2701_002081"
     CDS             9060..9584
                     /locus_tag="O2701_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293962.1"
                     /translation="MLYLNKYKERVTSVRIQNRWVVFILFLICSFGVLIGLYQYRHTK
                     TVDLSNLEINDIKLNEKFDKKGYEVNKKIKFDRFKFYNSKAHPDLTVKVREKDNIVKG
                     IILVRDEKIHTNFDGGIGSPINNAIENLGFGYKRTKVGNDFSSVKYIDRDNHLKLNLL
                     YQDLEIKRIEFFSK"
     gene            9644..10201
                     /locus_tag="O2701_002082"
     CDS             9644..10201
                     /locus_tag="O2701_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001669025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HCZ4293963.1"
                     /translation="MNKITYLNELETALDALPRHLRDQKMYEYERYFYEQELNGVDEG
                     EILKKLKDPQDVAAETKARSVIDYAESKPTFENISRAVAASLSLGILSIFVILIPVSI
                     VGLFVLALFLISLLLLFCPIILLASAISRGIVDSISNVFFAISYSGLGLVFIIVIFKI
                     LEYIYRLILKYLLWYIKTVKGSVRK"
     gene            10198..11040
                     /locus_tag="O2701_002083"
     CDS             10198..11040
                     /locus_tag="O2701_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HCZ4293964.1"
                     /translation="MKKFFFIGLLVFVVFFTAATIIWFSYDKNKYGTKQYDKTFKDDA
                     FDNVSINLDSTELRIKRGNQFRVKYDGDNDILINIVDKTLKISDKRSKTRGYAIDMNP
                     FHENKKTLTIEMPDKMIKRLNLSSGAGSVRISDVDLENTSIQSINGEVVIKNSNLDAL
                     DSKTNNSSTYISKSNIKNSNIKVVIGTLQIDKSQIKQSIFLNDHGDIEFKNMPSKVDA
                     KASTKQGDIRFKYDSKPEDTILKLNPGTGDSVVKNKTFTNGKVGKSDNVLEFYTIDGN
                     IKVE"
     gene            complement(11097..12137)
                     /locus_tag="O2701_002084"
     CDS             complement(11097..12137)
                     /locus_tag="O2701_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HCZ4293965.1"
                     /translation="MQQVTSDIMTFRGSHFELGVKTGKWLQQTPLLKNREKEWKKRVP
                     RFDIDVNETYQIFQTYAPQIWEELMGLQSILKMPTRQIILNFGHYRFTDLKESGCTVF
                     QGKDFMVRNYDYHPATYDGRYLLYQPTDSGLAQIGPVSRVTGRMDGMNESGLTMGYNF
                     MHRKKPANGFVCYMIGRLILENCRNVTEAIQLLKEIPHRSSFSYILMDKSLNHAIVEV
                     TPRSIDVRYDNICTNHFEILTHENRNYTKESKERLARTISQTNDNLDMTTAFKLFNNP
                     QYEIYSKLFKSWSGTIHTSMYQPETLTAYFTLGENAPPEIIDFKSWLDGQDLNITHFT
                     GNIDTDLTFANK"
     gene            12588..12761
                     /locus_tag="O2701_002085"
     CDS             12588..12761
                     /locus_tag="O2701_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293966.1"
                     /translation="MWNFIKCVFKFVFSLVAITTLVAGVGVVAFAYIFKKDFEDIERK
                     TKEIISDIESKNN"
BASE COUNT         4581 a         1640 c         2115 g         4432 t
ORIGIN      
        1 agataattta ttattaatat tcagttaatt caaaaaatag agaaagtctg gctataatta
       61 agttgcaaat acgaattaca caaaaaagga gagataattt atgaaattta agtcattgat
      121 tacaacaact ttagcattag gtgttttagc atcaacaggt gcaaacttta ataataatga
      181 agcgtctgcc gcagctaagc cattagataa atcatcaagt tcgttacacc atggatattc
      241 taaagtccat gttccatatg caatcactgt gaacggtaca agccaaaata ttttatcaag
      301 cttaacattt aataagaatc aaaatattag ttataaagat ttagaggata gagttaaatc
      361 agttttaaaa tcagacagag gtattagtga tatagattta agactatcga agcaagcgaa
      421 atatactgtt tactttaaaa atggaacaaa gaaagttatc gatttgaaag caggtattta
      481 cacagctgat ttaattaata caagtgaaat taaagctatt aatattaacg tagatactaa
      541 aaagcaagtt gaagataaaa agaaagataa agcaaattac caagttccat acacaatcac
      601 tgtgaacggt acaagccaaa atattttatc aaacttaaca tttaataaga atcaaaatat
      661 tagttacaaa gatttagagg ataaagttaa atcagtttta gaatcaaata gaggtattac
      721 cgatgttgat ttaagattat cgaagcaagc gaaatataca gttaatttta aaaatggaac
      781 gaagaaagtt atcgatttga aatcaggtat ttacacagcg aatttaataa attcaagtga
      841 tattaaaagt atcaatatta acgtagatac aaaaaaacat atcgaaaata aagctaaaag
      901 aaactatcaa gttccatatt caattaattt aaatggtaca tctacaaaca ttttatcgaa
      961 tctttcattt tcaaataaac cttggacaaa ttacaaaaat ttaactagtc aaataaaatc
     1021 agtactgaag catgatagag gtattagtga acaagattta aaatatgcta agaaagctta
     1081 ttatactgtt tattttaaaa atggtggtaa aagaatctta cagttgaatt caaaaaatta
     1141 cacagcaaac ttagttcatg cgaaagatgt taagagaatt gaaattactg ttaaaacagg
     1201 aactaaagcg aaagcagaca gatatgtacc atacacaatt gcagtaaatg gcacatcaac
     1261 accaatttta tcagatttaa aatttacagg tgacccacgt gtaggctaca aagatatctc
     1321 taaaaaagtt aaatcagtat tgaagcatga tagaggtatc ggggaacgtg aattaaaata
     1381 tgcaaaaaaa gcaacttaca cagtacattt taaaaatgga acgaaaaaag tgattaacat
     1441 aaattcaaat attagccaac tgaatctgct ttatgttcaa gatattaaaa agatagatat
     1501 tgatgttaaa acaggaacta aagcgaaagc ggatagctat gtaccatata caattgcagt
     1561 aaatggcaca tcaacaccaa ttttatcaaa acttaaaatt tcgaataaac aattaattag
     1621 ttacaaatat ttaaatgaca aagtgaaatc tgtattaaaa agtgaaagag gcatcagtga
     1681 tcttgactta aaatttgcga aacaagcaaa atatacagta tatttcaaaa atggaaagaa
     1741 acaagtagtg aatttaaaat cagacatctt tacacctaat ttatttagtg ccaaagatat
     1801 taaaaagatt gatattgatg taaaacaata cactaaatca aaaaaaaata aataaatcta
     1861 ataatgtgaa attcccagta acaataaata aatttgaaaa catagtttca aatgaatttg
     1921 tgttctataa tgcaagcaaa attacaatta atgatttaag tataaaactt aaatcagcaa
     1981 tggcaaatga tcaagggata actaaacatg acataggact tgctgaacgc gcagtgtata
     2041 aagtgtattt taaaaatggt tcgtcaaaat atgtagactt aaaaactgag tataaagatg
     2101 aaagagtatt taaagcaact gacattaaaa aggtagatat tgaacttaaa ttctaatctt
     2161 taattttata ataaggcatc tcacaatcgt ggggtgcctt ttacatttgt agagatgtga
     2221 tgcttgaatg tttgaagtga tttgccaaaa gttatataaa ttggttctat aataaatcgg
     2281 actgatagaa aaagtttttt acttttcatc tgtccgattt tttgattttg ttaggagaga
     2341 tgtataaaac taattaggta tagtgtttga caagttgctg aataaaatat atcttatgat
     2401 actttgaaag aaggaataat tttaaaaata aaaaaccaat aatccgagtc atactcatca
     2461 gattattggt tgaaattaat tatcttaagt catcaatcgc tttagcaatt gaatcaaata
     2521 catgtgggat atcttctttt tctacacagc taaacgcaat acgaatatct gttgcattaa
     2581 gtgcaataac accaattgaa tatttttcaa ttaaatgttt acgaagttct tcagggtcaa
     2641 catcatgaac ttttattgcc ataaagtatc cagagttaaa gtcataggct tgccaatgtg
     2701 aatgatattg atcagcatag acaacttcct tagtgacttc atagcgttct tttaatgttt
     2761 gaatattttg ttcgatttct ttatcgaatt gtttattatt ttttaataca tgcttaacag
     2821 cgctttgtgt tggaagaggt ccactagaaa tgttacttcg tataagacct tttactttgg
     2881 cttctaatac ttctttagtt gtttgatcag acgttccaaa tgtcataaac ccaacacgga
     2941 atccccatgc gaaaaattct tttgttgcac catctaaacg aacaggtaat attgcatttg
     3001 aatttaaatt agataatgca gtaaataatg attgagtata cacatcttca tagaataaac
     3061 catagtatgc gtcatccaca acagctataa cttttgtacc tttatcagct aatgctttaa
     3121 ttgcatcgac gatggtagtc acttctttat gcgtaggtgt gtaacctgtc ggattattag
     3181 gataatttaa aatcataatg actttatctt tattgtatga ttgtaaagct tctacaagtg
     3241 aatcagtagt ataatgcccg tctttatcaa agataggata tgtttgaagg tttgcaccat
     3301 ttctcgtatt gaaaacaagt ttgtaattac cccaattatg ctctggtaac aagatagtgt
     3361 caccttgatt tacaaataaa tcgccaacta aagataaacc atgtgttaat gcattcgtaa
     3421 caattggtag tgacatgttg tcgattgata gctctggatt gtcacgcaac attttttgtt
     3481 gccataaatc acgtaattct tcgatgcctt gtggtggcgc ataagggaat atttcatctg
     3541 gagttaaatc attaaacatt gcatctaaag atgatgcaaa catttttccg tctttgtttg
     3601 tcgccatacc tatagttgca ttatatgttg tactctttgc ttcagcagat tgagataaaa
     3661 tgccttttgg ataaaacata ttttgaccta agtcagataa cattgtaaag gcagttgcat
     3721 ttgactgttg aagttgttca tttaagcttt gggctaaagg attcattatt gcttaacctc
     3781 tctttaaaaa aattgtatgc ctatatatta tcttcaatta taccaaagat aaagtgttgt
     3841 tggtgaatta acgaataata tgtaaaatct gctgccaaaa tacgaacata tgttctgttt
     3901 ttatgttaga ataggtgtag gtcatccttc agtatgatac tttagtcgaa cggaggtgta
     3961 gtgtaatgat gtggtggcaa gaagggatga tgacacttat tacaggtggc ttactaatta
     4021 tttttcgact ttggttagaa ctgaaatgga aaaataaaaa atgatcctac acacataaat
     4081 aaccccaagt ctgctggtac cagacttggg gttgtgtagt ataattctgt ggatacactt
     4141 aaataatatc attgaacaca atatatcgca atcaataatc attttgattg aatggttttg
     4201 ttgcgctatt ttagttgaaa gtgattagta ccttgaactt ttgaaattca ataacttatt
     4261 aaaaaaattt atatttgtta taatgtcgat gttgaggtga aataaattga aacatctaac
     4321 aaaaatattt gtagtacttg caattatttt atttattatt ggttattatt tacaagcaac
     4381 taatcatgaa agccaaggta taaaattatt attagcagcg attatgttta tgatttgtgc
     4441 ttttataagt agaagtaatg atcgacgtaa aaatagtaaa tagaattgac tgtttaaaac
     4501 gattgtttat gttgacgcta cggttgtcac gtagagatta aagacattcg ttttagcagt
     4561 cttttttatt gaagtagaca atgcaagatt aaatggtagt gtcatttcat tttattttat
     4621 tcaactgcta gtaatacaat actcagtgtt atttctgaag actggttgat aatgagacat
     4681 gcatatttac aaaactgtgt atattgtgta tattgtatat atacagaagt tgataggggg
     4741 acgttgatga aaataatttt aaaaaacaat agtgattttc cgatttatga acagattaag
     4801 caacaagtaa aacaaaatat tttaaaggga catgttgctc ctggagagca tttgccgtca
     4861 atgagagaac ttgccaaaga tcttcaagta agtttgatta ctaccaaacg tgcttatgaa
     4921 gatttagaga aagacggttt tgttacaaca attagaggaa aagggacctt tgttaaggag
     4981 caagatagtt ctattttaaa agagaaacaa ttttttacca ttgaaaattt ggttaaagaa
     5041 ttggttaatg aagcgcaagc catcgaaatg tcacttgagg aactgcagga tattttaacg
     5101 ttcatttatg aggaggaatc atcatgaatg ccatagaatt aagtaatgtt aattattcaa
     5161 gtgatcagtt taatttaaag aatatctctt tcaaagttcc tcaaggattc gtcactggtt
     5221 ttattggtag aaatggcgct ggtaaaacca caataataag gttaattatg gatttgtatc
     5281 aaccacaaac tggtgttatt agagtattag aagaggatat ggctctcaat ccgatagagt
     5341 taaagaatag aattgggttt gtttactctg aaaattattt taatgagaga tggactacga
     5401 agcaacttga aaaaatgatt gccccttttt atcgtaaatg ggaccatcaa gtatttgaat
     5461 tttatcttga aaagttcgat ttaccaatca ataaatcaat aaaaacattt tcgactggga
     5521 tgaaaatgaa attatcacta gcagttgcat tttcacatca tgctgaattg tatatttttg
     5581 atgagcctac ttcaggtctt gatcccttag ctagaaatga gctattggag ataattcagc
     5641 aagaattaat tgatgaaaat aaaacaatat tcatgtcgac acacattatc tcagatttag
     5701 aaaaaatagc tgattatatc attcacttaa gtgacggtga agttatctta aatggttcaa
     5761 aagaacaact gttacaaaga tatcaagttg tgagtggtgc tattgaggac cttgatgatg
     5821 aattagcatc tctacttatt tatgaagaac ataagcgtac gggatttata ggattaacag
     5881 aacatgcgca agtatttaaa gaaattttag gtcataaagt gaatatcact acaccttcta
     5941 ttgagaactt aatggtttat cttgaaaaga gaaaacctaa atatcatgaa aacatcaaat
     6001 tgatggaaga aggtttttaa atgaaggcat tgttaattag gaattttaaa ttacgacgat
     6061 atacacttat catctatgtt ctattattaa ccttatatcc tttttatata atgcttgact
     6121 caacaaagtt cttttactta ttgcaatctt ttataagtcc aacaatattg attatttgga
     6181 ttttagatgc tggtcactta tttagactca atcgtagatt aggtggtaat gattcatatt
     6241 atttttatat gagtttacca gtgtcaaaaa aacaattgct taatgccaac tatattacat
     6301 gcattgtttt aacattaatt ggaacgcttg ttattagttt atatgcttat gaagcagatg
     6361 tgattgaacc aaattcaatc tatttttcaa ctgcatatgc atttgtcata tctaacttct
     6421 tgtctatacc aattgcattt agtcaattta cagaattgcg tagagtcaaa gtgccatatg
     6481 gtatatacgt gtttactatt atcattttag ttccattttt attttcaatt gcaatagtat
     6541 tggtgaatta ttttgttcta agtcaatcat cattcccaga tttatattca tatattttaa
     6601 atattggttt tctaattata agcattgtta tacttattgt taattatttt aaacagctca
     6661 ataaaataaa tactagaaaa tttaaaggag gcagtcgatg aaattagaac atattacaaa
     6721 aaaatacggc tcaaatgtcg ttttaaatga tattgatttt gactttggcg atagtagaat
     6781 tgtcggatta ataggaaaaa acggtgttgg taaaacaacc gttatgaaag taatgaatgg
     6841 taatattatt aaatttgatg gaaaagtaga tattgataat gcagataata tcggtttttt
     6901 aattgagcat cctaaattat atgataataa atcaggattg tataacttga aattatttgc
     6961 acaagtatta ggtaagggtt ttgataaagc atacacagac aaaattatag atgcatttgg
     7021 tatgagacct tatattaaaa agaaagttaa gaaatattca atggggatga agcaaaagtt
     7081 agcaattgca gtatctttaa tgaataaacc taaattttta atcttggatg agcctacaaa
     7141 tggtatggat ccagatggct caattgatgt gctgactaca attaagtctt tagtaaatga
     7201 acttgatatg agaattctaa tatcaagtca taagttagaa gatattgaat taatttgtga
     7261 tagagctgta tttttaagag acggacattt tgttcaagat gtaaacatgg aggaaggtgt
     7321 tgcatctgac acaacgatag ttactgttga tcataaagac tttgatagaa ctgaaaaata
     7381 tcttgcagag catttccaat tacaaaatgt cgacaaagca gacggacatt taatgatcaa
     7441 tgcacaaaaa aattatcaag ttatactaaa agcattatct gaattagata tttatccgaa
     7501 atatattgaa acacgtaaaa gttcattgcg tgatacgtac ttcaatataa atcaaagagg
     7561 tgataaataa tgagaatttt aaatttagtt aagtatgatt tttatagtat atttaaaagt
     7621 cctttaacat atttagcgat actagtcgta tctagtttga ttgcaactca aagtatactt
     7681 atggcaaatt cgatggataa cccgaaacat attattgtct atggatctgt atttgctgca
     7741 gcaaaatggt tattgttaat aattggatta atgtttgttg ttaagacaat tacgcgtgat
     7801 ttttcacaag gtacaattca actatatatg agtaaagtta aaacacgcgt tggatacatt
     7861 atttcgaaaa caatttcaat tattttaatt tcaatattat ttgcattaat tcattatgtg
     7921 attttgattg ttgtgcaggc atctagtaat ggaaaaaatt tggcgttttc taaatatgta
     7981 gataatttat ggttcttcct aatcttttta ctattctttg gcttgttttt attcttaatc
     8041 acacttgcat cacaaaaaac agcaatgata ttttcattag gtgtattttt agtactcatt
     8101 gtaccgttta ttaaaccttt tattacattt atcccaagat acggtgaaaa agttttagat
     8161 gcttttgatt atatcccttt tgcttactta actgataaaa tgattagctc taactttgat
     8221 tttagcaatt ggcaatgggt aatttcatta ggttctatag tgatattctt cattttgaat
     8281 atcttatatg tcgctaaaaa agacatttaa taaaaataat tttgaggttg ggaattttaa
     8341 attttcccaa cctcaaagtt tgtcttattg taaatttatt tattttctaa tttatttagg
     8401 atggaattat aaactgcttt ccaaaatgaa gcgtcagttt tatagcggtt tgatataact
     8461 aagtgtgttt ctttttctaa atctgcatag tctggatgat ctttgctcgg taatttatca
     8521 gcacgaacat cagttacaaa tttttggact tcatttgctc ttggtcccca aactgtttct
     8581 tgttcgaatt gatcattcaa gaatacgaag ataggaattg cacgtgattt accatttgtt
     8641 aaatattgat cgatcagttt tgtatcatca tctctatgga acacgcgtac ttctaaattt
     8701 aatgcttcac tgatgtgttt tagaattggg agattcatca ttgcatctcc acaccagtct
     8761 tcagtaatta ctaatacttt agaataattc atctctttta tttttttgat gcgtgaatca
     8821 tcttctggta actcaaatga ttgatagata ctgagaacgg tatcttgatt tgtcttcatt
     8881 ccatcaatgt attcatttaa gggttggcta tttttgaaat aagtttctaa atttgtcatt
     8941 gtaaaaacct cctttagcat ttacaacatt ataccaattt atagtaataa aaggtaatga
     9001 aataaattaa ttgcaaattc tttgttaatt tttgttaagg atgaaacggg aagcacctta
     9061 tgctatattt aaataagtac aaagaaaggg tgacatcagt gcgtattcaa aatcgctggg
     9121 ttgtgtttat attattttta atctgttctt ttggtgtatt aattggttta tatcaatatc
     9181 gtcatacgaa aactgtggat ttgtctaatc ttgaaataaa cgatattaaa ttaaatgaga
     9241 agtttgataa aaaaggctat gaagtaaata aaaaaattaa gtttgatcgt tttaaatttt
     9301 ataacagtaa agctcaccct gatcttaccg ttaaagtgag agaaaaggat aacatcgtta
     9361 aggggataat attagtaaga gatgaaaaga tacatactaa ttttgatggg ggaattggtt
     9421 cgccgataaa taacgcgatt gaaaatcttg gattcggata taaaagaaca aaagttggca
     9481 atgatttctc atcggtaaag tatattgata gagataacca tttaaaatta aacttacttt
     9541 atcaagattt agaaattaaa cgtattgaat tttttagtaa atagctttag gtcttaaagt
     9601 tataaaaaac gaatgaataa ttttattggg atgagtgacc tatatgaaca agattactta
     9661 tttaaatgaa ttagaaacag cacttgatgc gctacctagg catttaagag atcaaaaaat
     9721 gtatgaatat gaacgttatt tttatgagca agaacttaat ggcgttgatg aaggggaaat
     9781 tttaaagaag ttaaaagacc cacaagatgt tgcagctgaa acaaaagcta gaagtgtcat
     9841 tgattatgct gaatctaaac caacatttga aaatatttca agagctgttg ctgcttcatt
     9901 aagtttaggc attctatcta tttttgtcat ccttatacca gtatctatag ttggattatt
     9961 tgtattagca ttatttttaa tatcactttt gctgctgttt tgtccaatta ttttattagc
    10021 atcagcaata tccagaggaa ttgtggactc aattagtaat gtattttttg ccatatcata
    10081 ttcaggatta ggattagtat ttatcattgt catatttaag attttagaat acatttatcg
    10141 tttaatctta aaatatttac tttggtatat taaaactgtc aaaggaagcg ttagaaaatg
    10201 aagaaattct tttttattgg gcttttagtg tttgttgtct tttttacagc agcaaccatt
    10261 atttggttca gctatgataa aaacaaatat ggtactaaac aatatgataa aacattcaaa
    10321 gacgatgctt ttgacaatgt atctataaat ttggatagta cagaacttcg tataaaacgg
    10381 gggaatcaat ttagagttaa atatgatggt gacaatgata tattaattaa tatagtagat
    10441 aagacgttga agattagtga taaaaggtct aagacaagag gatatgcaat tgatatgaat
    10501 ccttttcatg agaataagaa aacgttaacg attgaaatgc ctgataaaat gattaaacgt
    10561 ttaaatctat catctggagc aggaagtgtt agaatcagtg atgttgattt agagaacaca
    10621 agtattcaaa gcattaacgg tgaagtagtt attaaaaact caaatcttga cgcactagat
    10681 tcaaagacaa ataatagttc tacttatatt agtaaaagta acattaaaaa tagcaatatt
    10741 aaagttgtta ttggtacgct acaaatcgac aagagtcaaa ttaaacaatc catattttta
    10801 aacgatcatg gtgacattga atttaaaaac atgccatcaa aagtagatgc aaaagcttct
    10861 actaaacaag gagatattcg ttttaagtat gatagtaaac ctgaagacac tatactaaag
    10921 ctaaatccgg gaacgggtga tagcgtagtt aaaaataaaa catttactaa tggtaaagtt
    10981 gggaaaagcg acaatgtttt agaattttat acgattgatg gtaatatcaa agttgaataa
    11041 ataaaggatg taagcaccga tattaggaag cataatttct ctaatatcgg tgttatttat
    11101 ttgttggcaa aagttaagtc ggtatctata ttgccagtaa agtgagtgat attaaggtct
    11161 tgaccatcta accatgattt gaaatctatt atttctggtg gcgcattttc tcccaatgta
    11221 aaatatgcag ttaatgtttc aggttgatac attgatgtat ggatggtgcc agaccagctt
    11281 ttgaatagtt tactgtaaat ttcatactga ggattattga ataacttaaa tgctgtagtc
    11341 atatctaaat tatcattagt ttgtgaaatg gtacgcgcca gtctttcttt agattctttt
    11401 gtataattac gattttcatg tgttaatatt tcaaaatgat ttgtacatat attatcataa
    11461 cgaacatcta ttgatctcgg tgtcacttca acaattgcat ggttcaatga tttgtccatc
    11521 agtatgtagc taaatgagct tctgtgtggt atttctttca ataattggat tgcttctgtt
    11581 acatttcggc aattttcaag aattagacga ccaatcatat aacatacaaa accatttgct
    11641 ggtttcttcc ggtgcataaa gttatagccc atagttaatc ctgactcatt cataccatcc
    11701 attcttccag ttacccttga tacaggacca atttgagcta aaccgctatc tgtaggttga
    11761 taaagtaagt agcgaccatc ataagttgca gggtggtaat cataatttct aaccatgaag
    11821 tctttgcctt gaaagaccgt gcaaccactt tcttttaaat cggtaaaacg ataatgtcca
    11881 aagtttaaaa taatttggcg tgttggcatt ttgagtatac tttgtagtcc cattaattct
    11941 tcccatattt gaggtgcgta tgtttggaat atttgataag tttcatttac atctatatcg
    12001 aaacgtggga cacgtttttt ccattctttt tctcgatttt ttagaagagg tgtttgttga
    12061 agccatttac cagttttaac acctaactcg aaatgtgaac ctctaaaagt catgatatct
    12121 gatgtcactt gttgcatatc atcggcccct ttctttttag ttgtaatata ttgtaaataa
    12181 atagtaatcg tatgtatatt gaatgtcatg ttaaataaag ttatatttta ctaaatgaaa
    12241 tataaaattg tttgaggtga tttctcggtg tataagactt atcaatcagt taaaacatat
    12301 ttttatagat ggtggggata ttgagttaaa aacttaaaat catcttatca taaatatcaa
    12361 tcttaagtta gcattcacga taatagtcat tgttaacatt agcatataag gtcatgtcac
    12421 gttgaaacag aggttcctcg gcatttttga aaataatcat tagcttgctc actatataat
    12481 ttgatgaata tatttcgtga aagtgggtat ttatttaatg attattctat atatgatagt
    12541 ataatgaaat gtagataggt atttaattta acagaggtga aattgagatg tggaatttta
    12601 ttaaatgtgt gtttaaattc gtatttagct tagttgctat tacaacatta gttgctggtg
    12661 ttggtgtagt agcatttgct tatatcttta aaaaagattt tgaagatatt gaaagaaaaa
    12721 ctaaagaaat tatttctgat attgaaagta aaaataacta ataacatt
//
LOCUS       DALAGD010000084        12304 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.085, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000084 DALAGD010000000
VERSION     DALAGD010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12304
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.085"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            36..833
                     /locus_tag="O2701_002086"
     CDS             36..833
                     /locus_tag="O2701_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HCZ4293967.1"
                     /translation="MRIMFIGDIVGKIGRDAIETYIPQLKQKYKPTVTIVNAENAAHG
                     KGLTEKIYKQLLRNGVDFMTMGNHTYGQREIYDFIDEAKRLVRPANFPDEAPGIGMRF
                     IQINDIKLAVINLQGRAFMPDIDDPFKKADQLVKEAQEQTPFIFVDFHAETTSEKYAM
                     GWHLDGRASAVVGTHTHIQTADERILPKGTGYITDVGMTGFYDGILGINKTEVIERFI
                     TSLPQRHVVPNEGRSVLSGVVIDLDKEGKTKHIERILINDDHPFSTF"
     gene            973..2733
                     /locus_tag="O2701_002087"
     CDS             973..2733
                     /locus_tag="O2701_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HCZ4293968.1"
                     /translation="MKPQLSWKVGGQQGEGIESTGEIFATAMNRKGYYLYGYRHFSSR
                     IKGGHTNNKIRVSTTPVHAISDDLDILIAFDQETIDVNHHEMREDSIILADAKAKPVK
                     PEGCHAQLIELPFTATAKELGTALMKNMVAIGATSALMNLNTNTFEELITNMFSKKGD
                     KVVEVNIQALNEGYQLMQSRLPEIYGDFELESTDALPHLYMIGNDAIGLGAIAAGSQF
                     MAAYPITPASEVMEYMIANISKVNGAVIQTEDEIAAVTMAIGANYGGVRAFTASAGPG
                     LSLMMEAIGLSGMTETPLVIINTQRGGPSTGLPTKQEQSDLMQMIYGTHGDIPKIVVA
                     PTDAEDAFYLTMEAFNLAEQYQCPVIVLSDLQLSLGKQTVEKLDYNRIEIKRGEIIQS
                     DIEREEDDKGYFKRYALTSDGVSPRPIPGVKGGIHHITGVEHNEEGKPSESASNRQQQ
                     MEKRMRKIEQLLIESPVEANLQHEDADILYIGFISTKGAIQEGSNRLNQQGIKVNTIQ
                     IRQLHPFPTSVIQDAVNKAKKVVVVEHNYQGQLASIIKMNVNIHDKIENYTKYDGTPF
                     LPHEIEEKGKIIATEIKEMV"
     gene            2734..3600
                     /locus_tag="O2701_002088"
     CDS             2734..3600
                     /locus_tag="O2701_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HCZ4293969.1"
                     /translation="MATFKDFRNNVKPNWCPGCGDFSVQAAIQKAAANIGLEPEEVAI
                     ITGIGCSGRLSGYINSYGVHSIHGRALPLAQGVKMANKDLTVIASGGDGDGYAIGMGH
                     TIHALRRNMNMTYIVMDNQIYGLTKGQTSPSSAVGFVTKTTPKGNIEKNVAPLELALS
                     SGATFVAQGFSSDIKGLTKLIEDAINHDGFSFVNVFSPCVTYNKINTYDWFKEHLTSV
                     DDIENYDSTDKQLATKTVIEHESLVTGIVYQDKETPSYESQIKELDDTPLAKRDIKIT
                     EDTFNALTEQFI"
     gene            3694..3987
                     /locus_tag="O2701_002089"
     CDS             3694..3987
                     /locus_tag="O2701_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HCZ4293970.1"
                     /translation="MKDTLMSIQIIPKTPNNDNVIPYVDEAIKIIDESGLHFRVGPLE
                     TTVQGNMNECLILIQSLNERMVELECPSIISQVKFYHVPDGITIETLTEKYDE"
     gene            4121..5665
                     /gene="miaB"
                     /locus_tag="O2701_002090"
     CDS             4121..5665
                     /gene="miaB"
                     /locus_tag="O2701_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCZ4293971.1"
                     /translation="MNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKR
                     GKQEVRYNRDFQIDEKYRGMGNERTFLIKTYGCQMNAHDTEVIAGILEALGYQATTDI
                     NTADVILINTCAIRENAENKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILK
                     SYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVN
                     IMYGCDKFCTYCIVPFTRGKERSRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDL
                     QDIEYDLGDLLQAISKIAIPRVRFTTSHPWDFTDHMIDVISEGGNIVPHIHLPVQSGN
                     NAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTDIIVGYPNESEEQFEETLTLYDEV
                     GFEHAYTYLYSQRDGTPAAKMKDNVPLNVKKERLQRLNKKVGHYSQIAMSKYEGQTVT
                     VLCEGSSKKDDQVLAGYTDKNKLVNFKAPKEMIGKLVEVRIDEAKQYSLNGSFIKEVE
                     PEMVIQ"
     gene            5666..6031
                     /locus_tag="O2701_002091"
     CDS             5666..6031
                     /locus_tag="O2701_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RicAFT regulatory complex protein RicA family
                     protein"
                     /protein_id="HCZ4293972.1"
                     /translation="MYNKDDVLNQADNIANKIKNLDTIKTYQQIEAQIHQNQTIKTKM
                     DMLKKHQKQAVNFQNYGKQNALEQSEHTIQSIEAEINTLPIVEQFQTSQYEANQLLKM
                     FVSTMETRLNDHNKAKHSD"
     gene            6058..6549
                     /gene="thiW"
                     /locus_tag="O2701_002092"
     CDS             6058..6549
                     /gene="thiW"
                     /locus_tag="O2701_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HCZ4293973.1"
                     /translation="MKSRKLAITALLIAINVVLSSIIIIPLGPVKAAPVQHFVNVLSA
                     VIVGPWYGLAQALISSILRVLFGTGTAFAFPGSMIGVLLASMFYIYRKHIFMAAVGEV
                     LGTGVIGSLICIPLAYFLGLQDFFIKPLMITFIVSSAIGSIISYFLLITLKKRGILQR
                     FIK"
     gene            6852..9470
                     /gene="mutS"
                     /locus_tag="O2701_002093"
     CDS             6852..9470
                     /gene="mutS"
                     /locus_tag="O2701_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCZ4293974.1"
                     /translation="MSNVTPMMQQYLKIKSEYQDCLLFFRLGDFYEMFYEDAKEASRV
                     LEITLTKRDAKKENPIPMCGVPYHSADSYIDTLVNNGYKVAICEQMEDPKQTKGMVRR
                     EVVRIVTPGTVMEQGGVDDKQNNYILSFVMNQPEIALSYCDVSTGELKVTHFNDEATL
                     LNEITTINPNEVVINDNISDNLKRQINMVTETITVRETLSSEIYSVNQTEHKLMYQAT
                     QLLLDYIHHTQKRDLSHIEDVVQYAAIDYMKMDFYAKRNLELTESIRLKSKKGTLLWL
                     MDETKTPMGARRLKQWIDRPLISKEQIEARLDIVDEFSAHFIERDTLRTYLNQVYDIE
                     RLVGRVSYGNVNARDLIQLKHSISEIPNIKALLNSMNQNTLVQVNQLEPLDDLLDILE
                     QSLVEEPPISVKDGGLFKVGFNTQLDEYLEASKNGKTWLAELQAKERQRTGIKSLKIS
                     FNKVFGYFIEITRANLQNFEPSEFGYMRKQTLSNAERFITDELKEKEDIILGAEDKAI
                     ELEYQLFVQLREEVKKYTERLQQQAKIISELDCLQSFAEIAQKYNYTRPSFSENKTLE
                     LVESRHPVVERVMDYNDYVPNNCRLDNETFIYLITGPNMSGKSTYMRQVAIISIMAQM
                     GAYVPCKEAVLPIFDQIFTRIGAADDLVSGKSTFMVEMLEAQKALTYATEDSLIIFDE
                     IGRGTSTYDGLALAQAMIEYVAETSHAKTLFSTHYHELTTLDQALPSLKNVHVAANEY
                     KGELIFLHKVKDGAVDDSYGIQVAKLADLPEKVISRAQVILSEFEASAGKKSSISNLK
                     MVENEPEINQENLNLSVEETTDTLSQKDFEQASFDLFENDQESEIELQIKNLNLSNMT
                     PIEALVKLSELQNQLK"
     gene            9483..11492
                     /gene="mutL"
                     /locus_tag="O2701_002094"
     CDS             9483..11492
                     /gene="mutL"
                     /locus_tag="O2701_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCZ4293975.1"
                     /translation="MGKIKELQTSLANKIAAGEVVERPSSVVKELLENAIDAGATEIS
                     IEVEESGVQSIRVVDNGSGIEAEDLGLVFHRHATSKLDQDEDLFHIRTLGFRGEALAS
                     ISSVAKVTLKTCTDNANGNEIYVENGEILNHKPAKAKKGTDILVESLFYNTPARLKYI
                     KSLYTELGKITDIVNRMAMSHPDIRIALISDGKTMLSTNGSGRTNEVMAEIYGMKVAR
                     DLVHISGDTSDYHIEGFVAKPEHSRSNKHYISIFINGRYIKNFMLNKAILEGYHTLLT
                     IGRFPICYINIEMDPILVDVNVHPTKLEVRLSKEEQLYQLIVSKIQEAFKDRILIPKN
                     NLDYVPKKNKVLHSFEQQKIEFEQRQNTENNQEKTFSSEESNSKPFMEENQNDEIVIK
                     EDSYNPFVTKTSESLIADDESSGYNNTREKDEDYFKKQQEILQEMDQTFDSNDGTTVQ
                     NYENKASDDYYDVNDIKGTKSKDPKRRIPYMEIVGQVHGTYIIAQNEFGMYMIDQHAA
                     QERIKYEYFRDKIGEVTNEVQDLLIPLTFHFSKDEQLVIDQYKNELQQVGIMLEHFGG
                     HDYIVSSYPVWFPKDEVEEIIKDMIELILEEKKVDIKKLREDVAIMMSCKKSIKANHY
                     LQKHEMSDLIDQLREAEDPFTCPHGRPIIINFSKYELEKLFKRVM"
     gene            11498..12040
                     /locus_tag="O2701_002095"
     CDS             11498..12040
                     /locus_tag="O2701_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCZ4293976.1"
                     /translation="MNQVNNNILPAIRNIKDLEKLIKTDYKMCVLLDMHIGHIKSIME
                     LLKQNHIECFIHIDLIKGLSHDEFASEFIIQQYKPKGIVSTKSKVIKKAKSLNTLTIF
                     RVFIIDSQALKRSIDLIKKVEPDFVEVLPGVASKAIHHIQKETNTQVIAGGLINTIDE
                     VNEAVKNGAKYVTTSYDKLW"
BASE COUNT         4705 a         1650 c         2302 g         3647 t
ORIGIN      
        1 aattaaacta gtatttatga gaggatgaac aaaacatgag aataatgttt ataggggata
       61 tcgtaggtaa aattggacga gacgcaattg aaacgtacat acctcaactg aagcaaaagt
      121 ataaaccaac agttacaatt gtaaatgctg aaaatgcagc acatggtaaa ggtttgactg
      181 aaaaaatata taaacaatta ctaagaaatg gtgtagattt catgactatg ggtaatcaca
      241 catatggtca acgtgaaatt tatgatttta tagatgaagc aaaacgacta gtaagaccag
      301 cgaattttcc ggatgaagcg ccgggaattg gtatgagatt tatacaaatt aatgatatta
      361 aacttgcagt tattaatctg caaggaagag cgtttatgcc agatattgat gatcctttta
      421 aaaaggcaga tcaattagtc aaggaagcac aagaacaaac tccgtttata tttgttgatt
      481 ttcatgcaga aacaacttct gaaaagtatg caatgggatg gcatttagat ggtagagcta
      541 gcgctgttgt tggaacgcat acacacattc aaacagcaga tgaacgtatt ttaccaaagg
      601 ggacagggta tataacggat gttggtatga caggttttta tgatggcatt ttaggaataa
      661 ataaaacaga ggtaattgag cgttttatca ctagtttgcc acaaagacat gttgttccaa
      721 atgaaggtag aagtgtatta tctggtgttg ttattgattt agacaaagaa ggtaaaacaa
      781 agcacatcga acgtatattg ataaatgatg accatccatt ttcaacattt taaaattacg
      841 taagtaaaca ttcgaattgg accctatcgt ccattagtat gaatttaata tagtaccact
      901 gtttacatag taaatcggtg gttctttttg ttatcattta atatgaaata tatccatagg
      961 aggcatataa ctatgaaacc acaattatcg tggaaagttg gcggtcaaca aggcgaaggt
     1021 attgaatcaa ctggggaaat cttcgctacg gctatgaata gaaaaggata ttatttatat
     1081 ggatatagac atttttcaag tcgtatcaaa ggtggacata cgaataataa aattagagtt
     1141 tctacgacgc ctgttcatgc aattagtgat gatttagata ttttgattgc atttgaccaa
     1201 gaaacaattg atgttaacca tcatgaaatg agagaagaca gtattatttt agctgatgcc
     1261 aaggctaaac ctgtgaaacc agaaggatgt catgcacagc ttattgaatt accttttaca
     1321 gcaaccgcta aagaattagg tacagcatta atgaaaaaca tggttgcaat aggtgctact
     1381 agcgcattga tgaatttgaa tacaaataca tttgaagaac ttattactaa tatgttttct
     1441 aaaaaaggtg acaaggtagt tgaagtcaat atccaagcat taaacgaagg ttatcaatta
     1501 atgcaatctc gcttacctga aatctacggg gactttgaat tagagtcaac agatgcacta
     1561 ccacatctat atatgattgg taacgatgcc attggattag gtgcaattgc tgcaggttca
     1621 caatttatgg cggcatatcc tattacacct gcgtctgaag ttatggaata tatgattgcc
     1681 aatatatcta aagtaaacgg agcggttatt caaacagaag atgaaattgc tgctgtaact
     1741 atggctattg gtgcaaatta tggtggagtc agagcgttta cggctagtgc tggtccaggt
     1801 ttatctttaa tgatggaagc aattggatta tctggtatga ctgaaacgcc attagtcatt
     1861 attaataccc aacgaggtgg accttctact ggattaccta cgaaacaaga acagtcagat
     1921 ttaatgcaaa tgatttatgg tacacatggt gatattccaa aaattgttgt agcaccaaca
     1981 gatgcagaag atgcatttta tttaactatg gaagcattta atttagcaga acaatatcaa
     2041 tgccctgtta tagttctaag tgatttgcaa ttatctttag gtaaacaaac tgttgaaaaa
     2101 ttagattata atcgtattga aattaaacgt ggtgaaatca ttcaatctga tattgaacgt
     2161 gaagaagatg ataaaggtta tttcaagcgt tatgcgttaa catccgatgg tgtttctcct
     2221 agacctatcc ccggtgttaa aggaggtatt catcatataa ctggtgtgga acacaatgaa
     2281 gaaggtaaac ctagtgaatc tgcgtcaaat agacaacaac aaatggaaaa acgaatgcgt
     2341 aaaattgagc agttactaat tgaatcgcca gtagaagcta acttacaaca tgaggatgca
     2401 gatattcttt atatcggttt tatttctaca aaaggtgcaa ttcaagaagg tagtaaccgt
     2461 ttgaatcaac aaggcataaa agttaacact atacaaatta gacaattgca tccattccca
     2521 acaagcgtta ttcaagatgc agttaataaa gcgaagaaag tcgttgtagt ggagcacaat
     2581 tatcaaggac aattggctag tattataaaa atgaatgtca atattcatga taagattgaa
     2641 aattatacaa agtatgatgg gacacctttc ctaccacatg aaatcgaaga aaaaggcaaa
     2701 ataattgcta ctgaaataaa ggagatggta tagatggcga catttaaaga ttttagaaat
     2761 aatgttaagc ctaactggtg ccccggatgt ggcgatttct cagtacaagc tgcaattcaa
     2821 aaagcagccg caaatatagg gttagaacct gaagaagtag ctatcatcac cggtatagga
     2881 tgttctggcc gtctttcagg atatattaat tcttatggcg ttcattctat tcacggacgt
     2941 gcattacctt tagctcaagg tgtaaaaatg gcgaataaag atttaactgt tattgcatcg
     3001 ggaggagatg gtgatggtta tgctataggt atggggcata caatccatgc tttaagaaga
     3061 aatatgaaca tgacgtatat agtcatggat aatcaaattt atggtttgac aaagggacaa
     3121 acatcgccgt catcagcagt aggatttgtt actaaaacaa cgccaaaagg taatatagaa
     3181 aaaaatgttg cgcctttaga attagcatta tcatctggtg ccacatttgt agcccaaggt
     3241 ttttcaagcg atattaaagg attaacaaaa ctaattgaag atgcaattaa tcatgatgga
     3301 ttttcattcg ttaatgtctt ttcaccatgt gtgacttata ataaaattaa cacatacgat
     3361 tggtttaaag aacatttaac aagtgttgat gacattgaaa attatgattc tacagataaa
     3421 caattagcga ctaaaactgt tattgaacat gaatctttag taactggtat tgtttatcaa
     3481 gataaagaaa caccatcata tgaatctcaa attaaagagt tagatgatac accacttgct
     3541 aaaagagata tcaaaattac tgaagacacc ttcaatgcat taactgaaca atttatttaa
     3601 taattaaaac tgagtcgtat aaatgtattt ataacagatc catttatgct actcagtttt
     3661 ttactattac aaaaaataaa ggagttttta aaaatgaaag acacattaat gagtatacaa
     3721 ataattccta aaacaccaaa caatgacaat gttatacctt acgtagacga ggcgattaaa
     3781 ataattgacg aatctggttt gcattttaga gtaggtccgt tagaaacgac agtacaagga
     3841 aatatgaatg aatgtttaat tttaatacaa tcattaaatg aacgaatggt ggaacttgaa
     3901 tgtccaagta ttattagcca agttaagttt tatcatgtgc cagatggcat cactattgaa
     3961 actttaactg aaaaatatga tgaataacat taaaagtgaa gtaaactgga tttgaattgg
     4021 cttgttagag atgacgtata actttaactg tttttgcact ttatagttaa atttaatata
     4081 attattaaat gatacgggca aatagaaagg attttgtaaa gtgaacgaag aacaaagaaa
     4141 agcaagttct gtagatgttt tagctgagag agataagaaa gcagaaaaag attatagtaa
     4201 atattttgaa catgtttatc agccgcctaa tttaaaagaa gcgaaaaaaa gaggtaaaca
     4261 agaagttcgt tataatagag atttccaaat tgatgaaaaa tatcgcggta tggggaacga
     4321 gcgtacattt ttaattaaaa catatggatg tcaaatgaat gcacatgaca ctgaggtcat
     4381 tgctggtata cttgaagcat taggctatca agcaacgact gatattaaca ctgcagatgt
     4441 tattttaatt aatacatgtg cgattagaga aaatgccgag aacaaagtgt ttagtgaaat
     4501 aggtaatttg aagcatttga aaaaagaacg acctgatatt ttaatcggtg tttgtggttg
     4561 tatgtcacaa gaagagtcag tagtgaataa aattttaaaa tcgtatcaaa atgtagatat
     4621 gatatttggt acacataata ttcatcattt accagaaatt ttagaagaag catacttatc
     4681 taaagcaatg gttgttgaag tatggtctaa agaaggagac gttattgaaa atcttccaaa
     4741 agtccgtgaa ggcaacatta aagcatgggt caatattatg tatggttgtg ataagttttg
     4801 tacatattgt attgttccat ttacaagagg taaagaacga agccgtagac ctgaagacat
     4861 tatagatgaa gtacgtgaac ttgctcgtga aggttacaaa gaaataacgc ttttaggtca
     4921 aaatgtaaat tcttatggta aagatttaca ggatatagaa tatgacttag gagatctttt
     4981 acaagcaatt tctaaaatag cgattccaag agttcgtttc acaacaagtc atccttggga
     5041 ctttacagat cacatgattg atgttatttc agagggtggt aatatcgttc cgcatatcca
     5101 cttgccagtt caatctggaa ataatgcagt attaaaaata atgggtagaa aatatacacg
     5161 agaaagttat ttggatttag taaaacgaat caaagataga attcctaatg tagcattaac
     5221 tacagatatt attgtagggt atccaaatga atcagaggaa caatttgaag aaactttaac
     5281 tctgtatgat gaagttggtt ttgaacatgc atatacgtac ttgtattcac aacgtgatgg
     5341 tacgcctgct gctaaaatga aagataatgt acctttaaat gtcaaaaagg aacgattgca
     5401 acgtttgaat aaaaaagttg gtcattattc acaaatagct atgagtaagt acgaaggaca
     5461 aactgtaaca gtactttgtg aaggaagtag taaaaaagat gatcaggttc ttgctggcta
     5521 cactgataaa aataagctag ttaatttcaa agcgcctaaa gaaatgattg gtaaactagt
     5581 ggaagtacga atagatgaag ctaaacagta ttcattaaat ggcagtttta taaaggaagt
     5641 agagccggaa atggtgattc aataaatgta taataaagat gacgtgttga accaagcgga
     5701 taatattgca aataaaatta aaaatttgga tactatcaaa acatatcaac aaattgaagc
     5761 acagattcat cagaaccaaa cgataaagac taaaatggat atgttaaaaa agcatcaaaa
     5821 acaagcagta aactttcaaa attacgggaa acaaaatgcg ctagaacagt cggaacatac
     5881 cattcagagt atagaagcag aaataaatac attgcccata gttgaacagt ttcaaacttc
     5941 acaatatgaa gcgaatcaat tattgaaaat gtttgtatca acaatggaaa cacgtttaaa
     6001 tgaccataat aaagccaagc atagtgatta attacaaaca aaaaggagaa caatcatatg
     6061 aaatcaagaa aactggctat aactgcactt ttaattgcaa taaatgttgt attaagcagt
     6121 attatcatca ttcctctagg accagttaag gcagcaccag tacagcattt tgtaaatgta
     6181 ttaagtgcgg tcatagtagg tccttggtat ggattagctc aagcgcttat atcatcaatt
     6241 ttaagagttc tttttggtac tggtacagct tttgcatttc cgggtagtat gattggagtt
     6301 ttattggcta gtatgtttta catatatcgt aaacatatat ttatggccgc ggtcggtgaa
     6361 gtacttggaa ctggtgtcat cggaagttta atttgtatac cattagcata tttccttggg
     6421 cttcaagact tcttcattaa accgttaatg attacgttca tagtctcaag tgctatcgga
     6481 tctattataa gttatttctt attaattact ctaaaaaaac gtggtattct tcaaaggttt
     6541 ataaaataat tttatgatat atgatttcaa acagagtcat tgttcatttt aatattgaac
     6601 gcccttactt aattaaatgt cttgaatgtt agaattttaa tttaattcta aacatgctat
     6661 tgacacattt tattaggttt gaggcgtttt cttatacatt taagatgctt cgtgattata
     6721 tggtatgaat aggtttgtaa aagtaagtat gttaaaatgt ataagttaaa taattaataa
     6781 ttaaaaagct tacaaacgaa tatattgtaa agacaaattt taaaaagaga caagggttgt
     6841 gaagaaataa tatgtctaat gttacaccaa tgatgcagca atatttaaaa ataaaatcag
     6901 aataccaaga ttgcttatta ttttttagac taggtgattt ctatgaaatg ttttatgaag
     6961 atgccaagga ggcatcacgt gtacttgaaa ttactttaac taaaagagat gctaaaaaag
     7021 aaaatccaat tccgatgtgt ggtgttccgt atcattctgc agatagttat atagatacac
     7081 ttgttaataa tggatataaa gtagctattt gtgaacagat ggaagatccg aaacaaacga
     7141 aaggtatggt tagacgtgag gtagtaagaa ttgtgactcc aggaactgtg atggagcaag
     7201 gtggtgtaga tgataaacaa aataactata ttttaagttt tgttatgaat caacctgaaa
     7261 ttgcgcttag ttactgtgat gtttctactg gcgaattaaa ggttacacat tttaatgatg
     7321 aagcgacttt attaaatgaa attacgacga taaaccctaa cgaagttgtt atcaatgaca
     7381 atatttccga taatttaaaa agacaaatta atatggtgac agaaacaata acagtcaggg
     7441 aaacgttatc atcagaaatc tatagtgtga atcaaactga acataaatta atgtatcaag
     7501 cgacacaatt attgctagat tatattcatc atacacaaaa acgtgattta tcgcatatcg
     7561 aggatgttgt tcaatatgca gctatagatt atatgaaaat ggatttttat gctaagagaa
     7621 accttgagtt aacggaaagc attcgattaa aatcaaaaaa aggaacgcta ctttggctaa
     7681 tggacgaaac gaaaacacca atgggagcac gccgcttaaa acaatggata gatagaccac
     7741 taataagtaa agaacaaatt gaagcacgat tagatatcgt tgatgaattt agtgctcatt
     7801 tcatagaaag agacacctta agaacatatc ttaatcaagt gtatgatatt gaacgtcttg
     7861 ttgggcgtgt tagttacgga aatgttaatg cgagagattt aattcaactt aaacattcca
     7921 tttctgaaat accgaatatt aaagcattac taaattctat gaatcagaat actcttgtac
     7981 aagttaatca actagaaccc cttgatgatt tacttgatat attagaacag agtttagtag
     8041 aagaaccacc aatttcagtt aaagatggcg gactattcaa agttggtttt aatacgcaat
     8101 tagatgaata tcttgaagct tcaaaaaacg gaaaaacatg gttagcagaa ttacaagcca
     8161 aagaaagaca acgtacagga ataaaatcat tgaaaataag ctttaataaa gtgtttggtt
     8221 attttataga aataacacgt gccaacttgc aaaattttga accaagtgaa tttggttata
     8281 tgaggaagca aacgttatcg aatgctgaac gttttataac tgatgaactt aaagaaaaag
     8341 aagatatcat tttaggtgcg gaagacaaag ccatcgaatt agaatatcaa ttatttgttc
     8401 agctacgtga agaagttaaa aaatatactg aacgtttaca acaacaagct aaaattattt
     8461 cagagctaga ttgtttacag agctttgcag aaattgctca aaaatataat tacactaggc
     8521 cttcatttag tgaaaataaa acattagaat tagtggaatc taggcaccca gtagtggaaa
     8581 gagtaatgga ttataatgac tatgtgccta ataattgtcg attagataat gaaacattta
     8641 tatatttaat tacaggtccg aatatgtctg gtaaatcgac atatatgaga caagttgcca
     8701 taattagtat aatggcccaa atgggagctt atgtcccttg taaagaggca gtgttaccta
     8761 tatttgatca aatattcact agaataggtg cggcagatga tttggtttca ggtaagagta
     8821 cgtttatggt agaaatgcta gaagcacaaa aggcattaac ttatgcaaca gaggatagtt
     8881 tgattatttt cgatgaaatt ggacgtggta cttcaacgta tgacggttta gctttagcgc
     8941 aggcaatgat agagtatgta gctgaaacat cacatgctaa aacgttattt tcaacacatt
     9001 atcatgaatt gacaacatta gatcaagcat taccaagtct aaaaaatgtt cacgtcgctg
     9061 ctaatgaata taaaggtgaa cttatattct tgcataaagt caaagatggt gcagttgacg
     9121 atagttatgg tattcaagtt gcgaaattag ctgatttacc tgaaaaagtt attagcagag
     9181 cacaagtgat tctaagcgag tttgaagcgt ctgctggtaa aaaatcatcg atatcaaatt
     9241 taaaaatggt cgaaaatgaa cctgaaatta atcaagaaaa tttaaactta agtgttgaag
     9301 aaacaactga tactttatct caaaaagact ttgaacaagc atcatttgat ttgtttgaaa
     9361 atgatcaaga aagcgagatt gaactacaaa ttaaaaattt gaatttatct aatatgacac
     9421 caattgaggc attggtgaag ttaagtgaat tacaaaatca attaaaatag aggtgttgca
     9481 aaatggggaa aattaaagaa ctccaaacct cattagcaaa taaaatcgca gcaggtgaag
     9541 tagttgaaag accgagttct gttgtgaaag aactgttgga aaatgctata gatgcaggcg
     9601 ctacagaaat aagcattgaa gtagaggaat ctggcgtcca atctattcgc gtagtcgata
     9661 atggaagcgg aattgaagcg gaagacttag gattagtatt tcatagacat gcgactagta
     9721 aattagatca agatgaagat ttatttcata ttaggacatt aggattccgt ggtgaagcac
     9781 tagccagtat ttcatcagtt gctaaagtaa cattgaagac ttgcacggat aatgctaatg
     9841 gaaatgaaat atatgtagaa aatggtgaaa tattaaatca taagcctgca aaagcgaaaa
     9901 aaggaacaga tatacttgta gaatcattat tttataatac accagcacgt ttaaaatata
     9961 ttaaaagttt atacactgaa ctaggtaaaa taacagatat tgtcaacaga atggcaatga
    10021 gccatccgga cattcgaata gcactcattt cagatggcaa aacaatgtta agtacaaatg
    10081 gttcaggacg aactaatgaa gtgatggcag agatttatgg gatgaaagtt gcacgagatt
    10141 tagtacatat atctggagat acaagtgatt atcacattga aggttttgtt gcaaagcctg
    10201 aacattctag aagtaataag cactatattt ctatttttat taatggacga tacattaaaa
    10261 actttatgct aaataaagcg attttagaag gctatcatac actcttaaca ataggtaggt
    10321 tcccgatttg ttatattaat attgaaatgg atccaatctt agtagacgta aatgttcatc
    10381 caacaaaact agaagtgcgt ttatcaaaag aagagcaact ataccaattg attgtgagca
    10441 aaatacaaga agcatttaaa gaccgtatat taattcctaa aaataacttg gattatgtgc
    10501 caaaaaaaaa taaagtatta cattcattcg aacaacaaaa aatcgaattt gaacaaagac
    10561 aaaacacaga gaataaccaa gagaagacgt tttcatctga agaaagtaac agtaagccat
    10621 ttatggaaga aaatcaaaac gatgagatag ttataaaaga agattcatat aatccattcg
    10681 taacgaaaac atctgaaagt ttaatagctg atgatgaatc ttctggttat aataatacac
    10741 gtgaaaaaga tgaagactac ttcaaaaaac aacaagaaat tttacaagaa atggatcaaa
    10801 catttgattc aaatgatggt acaactgtgc aaaattatga gaataaagcg tctgatgatt
    10861 attatgatgt aaacgatatt aaaggaacaa aaagtaaaga ccctaaacga agaattccat
    10921 atatggaaat tgttggacaa gtacatggaa cgtatattat tgctcaaaat gaatttggca
    10981 tgtacatgat tgaccagcat gcagctcaag aaagaataaa atatgaatat tttcgagata
    11041 aaataggtga ggttaccaat gaagtacaag atttattaat cccgttaaca tttcattttt
    11101 caaaagatga acaattagtc attgatcaat ataaaaatga gcttcaacaa gtaggtatca
    11161 tgttagaaca ttttggtggt catgattata ttgtaagtag ctatccagtt tggttcccta
    11221 aagatgaagt agaagaaatt attaaagata tgattgagct aattttggaa gagaaaaaag
    11281 tagatatcaa aaaattacgt gaagatgtag caatcatgat gtcatgtaaa aaatccatta
    11341 aagcgaatca ttatttacaa aaacatgaaa tgtctgattt aattgatcaa ttaagagaag
    11401 cggaagatcc atttacatgt ccacatggtc gtccaataat cattaatttt tcaaaatacg
    11461 aattagaaaa attatttaag cgtgtgatgt agagaggatg aatcaagtga ataacaacat
    11521 attgcctgct ataagaaaca ttaaagattt agagaaactg attaaaacag actataaaat
    11581 gtgtgtgctt ctagatatgc atataggaca tataaaaagt attatggaat tgctgaagca
    11641 aaatcatata gagtgtttta ttcatataga tttgataaaa ggtttaagcc acgatgaatt
    11701 tgcaagtgaa tttattattc agcaatacaa gccaaaaggt atcgtatcga ctaaatctaa
    11761 agtaataaaa aaagctaaat cattaaatac tttaacgatt tttagagtat ttattattga
    11821 tagtcaagca ttgaaacgca gtatagattt gataaaaaaa gttgaacctg attttgttga
    11881 agtacttcca ggtgttgcga gtaaagcgat tcatcatatt cagaaagaaa caaacacaca
    11941 agtcattgca ggtggcctaa ttaatacaat agatgaagtc aatgaagctg ttaaaaatgg
    12001 agcgaaatat gtaactacta gttatgataa actttggtaa atcataagtc tgcaaaaatt
    12061 tcatttttca cgagatttag tcatataaca taataaaaaa gccgttttca tatgggatat
    12121 gcagtaggta tcaaacaaaa aataatcaaa taaaataatt agcacctcta aaacgattaa
    12181 aatcattaaa gttttagagg tgtttattag taaaaaatgc aactagtcat aaaaaatatg
    12241 gctgaagaat gtcaattaaa caaaagagcg tcataaataa attagttagg attggtattt
    12301 ctag
//
LOCUS       DALAGD010000085        11966 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.086, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000085 DALAGD010000000
VERSION     DALAGD010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11966
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.086"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            352..1752
                     /locus_tag="O2701_002096"
     CDS             352..1752
                     /locus_tag="O2701_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ4293977.1"
                     /translation="MDFNKENINMVDAKKAKKTVVATGIGNAMEWFDFGVYAYTTAYI
                     GANFFSPVENADIRQMLTFAALAIAFLLRPIGGVVFGIIGDKYGRKVVLTSTIILMAF
                     STLTIGLLPSYDQIGLWAPILLLLARVLQGFSTGGEYAGAMTYVAESSPDKRRNSLGS
                     GLEIGTLSGYIAASIMIAVLTFFLTDEQMASFGWRIPFLLGLFLGLFGLYLRRKLEES
                     PVFENDVATQPERDNINFLQIIRFYYKDIFVCFVAVVFFNVTNYMVTAYLPTYLEQVI
                     KLDATTTSVLITCVMAIMIPLALMFGKLADKIGEKKVFLIGTGGLTLFSIIAFMLLHS
                     QSFVVIVIGIFILGFFLSTYEATMPGSLPTMFYSHIRYRTLSVTFNISVSIFGGTTPL
                     VATWLVTKTGDPLAPAYYLTAISVIGFLVITFLHLSTAGKSLKGSYPNVDNEQDRAYY
                     AEHPKEALWWVKERKN"
     gene            2296..3672
                     /locus_tag="O2701_002097"
     CDS             2296..3672
                     /locus_tag="O2701_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HCZ4293978.1"
                     /translation="MNVILEQLKTHTQNKPNDIALHIDDETITYSQLNARITSAVESL
                     QKYSLNPVVAINMKSPVQSIICYLALHRLHKVPMMMEGKWQSTIHRQLIEKYGIKDVI
                     GDTGLMQNIDSPMFIDSTQLQHYPNLLHIGFTSGTTGLPKAYYRDEDSWLASFEVNEM
                     LMLKNENAIAAPGPLSHSLTLYALLFALSSGRTFIGQTTFHPEKLLNQCHKISSYKVA
                     MFLVPTMIKSLLLVYNNEHTIQSFFSSGDKLHSSIFKKIKNQANDINLIEFFGTSETS
                     FISYNLNQQAPVESVGVLFPNVELKTTNHDHNGIGTICIKSNMMFSGYVSEQCINNDE
                     WFVTNDNGYVKEQYLYLTGRQQDMLIIGGQNIYPAHVERLLTQSSSIDEAIIIGIPNE
                     RFGQIGVLLYSGDVTLTHKNVKQFLKKKVKRYEIPSMIHHVEKMYYTASGKIAREKMM
                     SMYLRGEL"
     gene            3674..4813
                     /locus_tag="O2701_002098"
     CDS             3674..4813
                     /locus_tag="O2701_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HCZ4293979.1"
                     /translation="MNQAVIVAAKRTAFGKYGGTLKHLEPEQLLKPLFQHFKEKYPEV
                     ISKIDDVVLGNVVGNGGNIARKALLEAGLKDSIPGVTIDRQCGSGLESVQYACRMIQA
                     GAGKVYIAGGVESTSRAPWKIKRPHSVYETALPEFYERASFAPEMSDPSMIQGAENVA
                     KMYDVSRELQDEFAYRSHQLTAENVKNGNISQEILPITVKGEIFNTDESLKSHIPKDN
                     FGRFKPVIKGGTVTAANSCMKNDGAVLLLIMEKDMAYELGFEHGLLFKDGVTVGVDSN
                     FPGIGPVPAISNLLKRNQLTIENIEVIEINEAFSAQVVACQQALNISNTQLNIWGGAL
                     ASGHPYGASGAQLVTRLFYMFDKETMIASMGIGGGLGNAALFTRF"
     gene            4788..5153
                     /locus_tag="O2701_002099"
     CDS             4788..5153
                     /locus_tag="O2701_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraC"
                     /protein_id="HCZ4293980.1"
                     /translation="MQHYLLDSNQRLNVSFSKDSVAAYYQCFNQPYRKEVPPLMCASL
                     WPKFDLFKKYANSELILTKSAINQTQKIEVDTIYVGHLEDIECRQTRNITRYTMALTL
                     TKNDQHVITVTQTFIKAMK"
     gene            5156..5425
                     /locus_tag="O2701_002100"
     CDS             5156..5425
                     /locus_tag="O2701_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293981.1"
                     /translation="MKFNEIWINEYLALVNDDNPIHNEIVPGQLVSQMMLMAMSLETN
                     QCQINYVKPILINENIEFIEQHEHEIIAINDDGEIKIKISLSTKK"
     gene            complement(5626..5793)
                     /locus_tag="O2701_002101"
     CDS             complement(5626..5793)
                     /locus_tag="O2701_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraX"
                     /protein_id="HCZ4293982.1"
                     /translation="MIIYRQYHHEGAPVYEIITKTFQHVSIKCDDSFSDTEIFKLLSL
                     LQDDIDHMKVS"
     gene            complement(6300..7130)
                     /gene="thiD"
                     /locus_tag="O2701_002102"
     CDS             complement(6300..7130)
                     /gene="thiD"
                     /locus_tag="O2701_002102"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCZ4293983.1"
                     /translation="MALKKVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTM
                     DKDTWSHDVTPLPMDVFEKQLETALSIGPDAIKTGMLGTEEIIKRAGEVYEASNAQYF
                     VVDPVMVCKGEDEVLNPGNTEAMIKYLLPKATVVTPNLFEAGQLSGLGKLNSIEDMKK
                     AATIIFDKGAQHVIIKGGKALDQDKSYDLYYDGQTFYQLTTDMFQQSYNHGAGCTFAA
                     ATTAYLANGKSPKEAVISAKAFVASAIKNGWKMNDFVGPVDHGAYNRIEHIDVEVTEV
                     "
     gene            7314..7970
                     /locus_tag="O2701_002103"
     CDS             7314..7970
                     /locus_tag="O2701_002103"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCZ4293984.1"
                     /translation="MEWSQIFHDITTKHDFKAMHDFLEKEYSTAIVYPDRENIYQAFD
                     LTPFENIKVVILGQDPYHGPNQAHGLAFSVQPNAKFPPSLRNMYKELADDIGCVRQTP
                     HLQDWAREGVLLLNTVLTVRQGEANSHRDIGWETFTDEIIKAVSDYKEHVVFILWGKP
                     AQQKIKLIDTSKHCIIKSVHPSPLSAYRGFFGSKPYSKANAYLESVGKSPINWCESEA
                     "
     gene            7971..8351
                     /locus_tag="O2701_002104"
     CDS             7971..8351
                     /locus_tag="O2701_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5327 family protein"
                     /protein_id="HCZ4293985.1"
                     /translation="MLNRETLIARIEQELVQAEQAQHDHDFEKHMYAIHILTSLYAST
                     SNTPHIGEQQMNRRIANHNQMPQSQITQPTHQVTVAEIEAMGGKVNTHSAHHHNKSYS
                     QPSNQQQRLATDDDIGNGESIFDF"
     gene            8484..8852
                     /locus_tag="O2701_002105"
     CDS             8484..8852
                     /locus_tag="O2701_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCZ4293986.1"
                     /translation="MKLFIILGALNAMMAVGTGAFGAHGLQGKISDHYLSVWEKATTY
                     QMYHGLALLIIGVISGTTSINVNWAGWLIFAGIIFFSGSLYILVLTQIKVLGAITPIG
                     GVLFIIGWIMLIIATFKFAG"
     gene            8973..10457
                     /locus_tag="O2701_002106"
     CDS             8973..10457
                     /locus_tag="O2701_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCZ4293987.1"
                     /translation="MEKKNKQIDRGDLKQNLSEKFVWAIAYGSCIGWGAFILPGDWIK
                     QSGPIAASIGIVIGALLMILIAVSYGALVERFPVSGGAFAFSFLSFGRYVSFFSSWFL
                     TFGYVCVVALNATAFSLLVKFLLPDVLNNGKLYTIAGWDVYITEIIIATVLLLVFMLV
                     TIRGASVSGSLQYYFCVAMVIVVLLMFFGSFFGNNFALENLQPLAEPSKGWLVSIVVI
                     VSVAPWAYVGFDNIPQTAEEFNFAPNKTFKLIVYSLLAASLTYVVMILYTGWLSTSHQ
                     SLNGQLWLTGAVTQTAFGYIGLGVLAIAIMMGIFTGLNGFLMSSSRLLFSMGRSGIMP
                     TMFSKLHSKYKTPYVAIIFLVGVSLIAPWLGRTALTWIVDMSSTGVSIAYFITCLSAA
                     KLFSYNKQSNTYAPVYKTFAIIGSFVSFIFLALLLVPGSPAALTAPSYIALLGWLIIG
                     LIFFVIRYPKLKNMDNDELSRLILNRSENEVDDMIEEPEKEKTK"
     gene            complement(10599..11051)
                     /locus_tag="O2701_002107"
     CDS             complement(10599..11051)
                     /locus_tag="O2701_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCZ4293988.1"
                     /translation="MFWILNFIFSFLASMFFCVIFDAPRKLYLSCGFVGTCGWMVYTL
                     FFNGFNVHTIYSSFFGSLALGLLSHYMARKQKEPAIIFMVTGIIPLVPGGLAYDATKN
                     LVLLNFSTAINTMLEVTLIAGAIALGLLFADQISKLIVSGFVKSFKRL"
     gene            complement(11064..11828)
                     /locus_tag="O2701_002108"
     CDS             complement(11064..11828)
                     /locus_tag="O2701_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCZ4293989.1"
                     /translation="MDINSEEYKQEVLIKDVVMLAARILLESGAEGTRVEDTMTRIAK
                     KLGYSESNSFVTNTVIQFTLHSESFPRIFRITSRDTNLIKISQANKISRQITNNEISL
                     AEAKTQLEKIYVAKRDSSLPFKGFAAAMIAMSFLYLQGGRLIDVLTAILAGSLGYLVT
                     EILDRKLHAQFIPEFIGSLVIGIIAVIGHTLIPTGDLATIIIAAVMPIVPGVLITNAI
                     QDLFGGHMLMFTTKSLEALVTAFGIGAGVGSVLILV"
BASE COUNT         4055 a         1833 c         2171 g         3907 t
ORIGIN      
        1 atttattttt gtaaaaataa attaaagtaa tgacaaagtt attgaagtaa attgagtata
       61 aacatttaaa tacgatgtcg aaaatggcga tagcatatca cttacatgaa gttatgtgct
      121 atcgctattt ttagttataa ttccaaaaag ttaatcgttc gatgatttaa gaattattat
      181 tgtttaattc aaatgtatga gggtataaaa tcattgaatt taattcgata aagcgaaatt
      241 tttgaacaaa catacttttg tatttatata aaagtttaaa ttcttataaa tttgacaaaa
      301 ctaattaact ccgtataatt atgaaacata caagagggag tgtatgaatt catggatttt
      361 aataaagaga atattaacat ggtggatgca aagaaagcta aaaaaaccgt tgttgcaacc
      421 ggtatcggta atgcaatgga atggttcgat tttggtgtct atgcatatac aactgcgtac
      481 attggagcga acttcttctc tccagtagag aatgcagaca ttcgacaaat gttgactttc
      541 gcagcattag ccattgcgtt tttattaaga ccaattggtg gtgtcgtatt tggtattatt
      601 ggtgacaaat atggacgtaa agttgtatta acatctacaa ttattttaat ggcattttca
      661 acattaacca ttggattatt gccaagctat gatcaaattg gactttgggc accaatacta
      721 ttattgcttg caagagtact acaagggttt tcaacaggtg gagagtatgc gggggcaatg
      781 acatatgttg ccgaatcatc tccagataag cgtcgtaact cattaggtag tggactagaa
      841 attgggacat tatcaggtta catagctgct tcaattatga ttgctgtatt aacattcttt
      901 ttaacagatg aacaaatggc atcatttggt tggagaatcc cattcttact cggtttattc
      961 ctaggattat tcggcttata tttacgtcgt aagctggaag aatcaccagt tttcgaaaat
     1021 gatgttgcaa cacaaccaga aagagataac attaactttt tacaaatcat cagattttat
     1081 tacaaagata tatttgtatg ttttgtagct gttgtattct tcaatgttac aaactatatg
     1141 gtaactgcat atttaccaac ctatttagaa caagttatta aattagatgc aacgacaaca
     1201 agtgtattaa ttacttgtgt catggcaata atgattccat tagcattaat gtttggtaag
     1261 ttagcggata aaataggtga aaagaaagta tttctaattg gtactggtgg gctaacatta
     1321 ttcagtatca tcgcatttat gttattacat tcacaatcat ttgttgtaat agtaatcggt
     1381 atatttatat taggattttt cttatcaact tacgaagcga caatgccagg gtcgttacca
     1441 acgatgtttt acagtcatat aagatatcga actttatcag taacatttaa tatctctgtt
     1501 tcgatatttg gtggtacgac gccattagtt gcaacatggt tagttacgaa aactggagat
     1561 ccattagcac ctgcgtatta tttaacagca atcagtgtta ttggcttttt agttattaca
     1621 ttcttacatt taagtacagc aggaaaatct ctaaaaggtt cgtatccaaa tgtagataac
     1681 gagcaagata gagcttatta tgcagaacat ccaaaagaag cattatggtg ggttaaagaa
     1741 cgtaagaatt agagatttta ataaaaagta taaatcaatc gtatataagc actttaaagc
     1801 tagtaggttc tgctaacttt aaagtgcttt ttaaattgag aactgtaatt agccgtaata
     1861 aagtttttgt atatacataa acccccactg caatgattat cgcaatgggg gaaagagggg
     1921 acttaaagca tatgtttagc tttgaatact taaaattctc ttgctattga aatgttagga
     1981 tgtaaatatg tcttagagta ttttgtccaa cgcaattaat attgagactc taaccttcaa
     2041 tattattata gagaacacaa acttaaatag attgggtgac ttatttgtgt cagttattgc
     2101 gattgcgata acttcttttc tctatataca tatagtaacg tcttatctaa taaaaaacat
     2161 ggtactacag tatcaaattt atctagggct taagtttgat ttttataata ggcaggttta
     2221 cctgataaaa atacttattc attatataat gttaacaata tgtattttaa agtttacatt
     2281 gagtgaggga tattgatgaa cgtaatttta gaacagttga aaacacatac tcaaaataaa
     2341 cctaatgaca tagcattaca tatcgatgat gaaacaatta catatagtca actaaatgcc
     2401 cgcatcacta gcgcagttga atctttgcag aaatattcac ttaaccctgt cgttgctatt
     2461 aatatgaaat caccggtgca aagtattatt tgttatttag ctttgcatcg tttacataaa
     2521 gtgcctatga tgatggaagg taaatggcaa agtactatac atcgtcaatt gattgaaaaa
     2581 tatggtatta aagatgtaat tggagataca ggtctcatgc agaatataga ctcaccgatg
     2641 tttattgatt caacgcaatt acagcactac cccaatttat tacatattgg ttttacttca
     2701 gggacaactg gactgccaaa agcatattat cgtgatgaag attcatggtt ggcttctttt
     2761 gaagttaatg aaatgttgat gttaaaaaat gaaaatgcaa tagcagcccc tggaccacta
     2821 tcgcactcgt taacattata tgcgttattg tttgctttaa gttccggtcg tacttttata
     2881 ggacagacca cttttcatcc tgaaaagtta cttaatcaat gtcataaaat atcatcatac
     2941 aaagttgcta tgtttcttgt tccaacgatg attaaatcat tattgttagt ttacaacaat
     3001 gaacatacaa tccaatcatt ttttagcagt ggagataagc tgcattcttc tatttttaaa
     3061 aagataaaaa atcaagcaaa tgacataaat ttgattgaat tttttggtac atcggaaacc
     3121 agttttatca gctataactt gaatcagcaa gcaccagttg aatcagtagg tgtgctattt
     3181 ccaaatgtgg aattgaaaac aacgaatcac gatcacaatg gtataggaac tatttgtata
     3241 aaaagtaata tgatgtttag tggctatgta agtgaacaat gtataaataa tgatgaatgg
     3301 tttgttacta atgataatgg ctatgtaaaa gagcagtatt tatatttaac gggacgtcaa
     3361 caggatatgt taattattgg tggtcaaaat atatatccag cacatgttga acgcctttta
     3421 acgcaatctt cgagcattga tgaagcaatt atcatcggta ttccaaatga gcgttttggt
     3481 caaataggcg tattgcttta ttctggtgat gtgacactta cacataaaaa tgtaaaacaa
     3541 tttttaaaaa agaaagtgaa acgctatgaa attccatcga tgattcatca tgtagaaaag
     3601 atgtattaca ctgcaagtgg taaaattgct agagaaaaaa tgatgtcgat gtatttgaga
     3661 ggtgaattat aatatgaatc aagcagtcat agttgcagct aaacgaactg catttgggaa
     3721 atatggtggc actttaaaac atttagagcc agaacaattg cttaaacctt tattccaaca
     3781 ttttaaagag aagtatccag aggtaatatc taaaatagat gatgtagttt taggtaatgt
     3841 tgttgggaat ggtggcaata ttgcaagaaa agcattgctt gaagcggggc ttaaagattc
     3901 aatacctggc gtcacaatcg atcggcaatg tgggtctgga cttgaaagtg ttcaatatgc
     3961 atgtcgcatg atccaagccg gagctggcaa ggtatatatt gcaggtggtg ttgaaagtac
     4021 aagtcgagca ccttggaaaa tcaaacgacc gcattctgtg tacgaaacag cattacctga
     4081 gttttatgag cgtgcatcat ttgcacctga aatgagcgac ccatcaatga ttcaaggtgc
     4141 tgaaaatgtg gccaagatgt atgatgtttc aagagaatta caagatgaat ttgcttatcg
     4201 aagtcatcaa ttgacagcgg aaaatgtaaa aaatggaaat atttctcagg aaatattacc
     4261 tataaccgtt aaaggagaaa tattcaacac tgatgaaagt ctaaaatcac atattccgaa
     4321 agataacttt ggccgattta agcccgtgat caaaggtggg accgttaccg ctgcgaatag
     4381 ttgtatgaaa aatgatggtg cagttttatt gcttattatg gaaaaagata tggcatacga
     4441 attaggtttc gagcatggtt tattatttaa agatggtgtt acggtaggtg ttgattctaa
     4501 ttttcctggc attggtccag taccagccat ttccaactta ctaaaaagaa atcaattaac
     4561 gatagaaaat attgaagtca ttgaaattaa cgaagcgttc agtgcacagg tagttgcctg
     4621 ccaacaagct ttaaatattt caaatacgca attaaatata tggggtggtg cattagcatc
     4681 aggtcatcca tacggtgcaa gcggtgccca attagtgact cgattatttt atatgtttga
     4741 caaagagact atgattgcat ctatggggat agggggaggt ctaggaaatg cagcattatt
     4801 tactcgattc taaccagcga ttaaatgtgt cattttctaa ggatagtgtg gctgcatatt
     4861 atcagtgttt taaccaacct tatagaaaag aagtaccacc attaatgtgt gcgtcattat
     4921 ggccaaaatt tgatttattt aaaaaatatg caaatagcga actgatttta acaaaatcag
     4981 caattaatca aactcaaaag atagaagtag acacaatata tgtagggcat ttagaagata
     5041 ttgaatgccg acagactcgc aatatcacac gttatacaat ggctttaaca ttaactaaaa
     5101 atgatcaaca tgtcataacg gttacacaaa cttttattaa ggcgatgaag tagagatgaa
     5161 gtttaatgag atatggataa atgaatattt ggcgctcgta aatgatgata atccaataca
     5221 taatgagatt gtgccaggac aattagtgag tcaaatgatg ctgatggcta tgtcattaga
     5281 gacaaaccag tgtcaaatta actacgttaa acctatttta ataaatgaaa atatcgaatt
     5341 cattgaacaa cacgaacacg aaattatagc aattaatgac gatggagaga ttaaaataaa
     5401 aatttctttg agcacaaaaa aataaccgat attagctgca tgaacgcata ttaattagga
     5461 gatgaaagga cagctaatat cagttatgta ttgttattat tattgggaac agagatgaat
     5521 ataggttacg tttctttctt tgcacgggga tgcattaatc taaaataata ataacaacta
     5581 tatcaatgtt taataaattc tggattattg gaacgattag tcaatttaac taactttcat
     5641 atgatctata tcgtcttgta ataaagagag caatttgaat atttcagtat cactaaatga
     5701 atcgtcacat ttaattgaaa catgctgaaa cgttttggtt ataatttcat aaactggtgc
     5761 gccttcatgg tgatactgtc gataaataat cataacctat attacctcct ttgctactct
     5821 atggttatat tataaataac atttttatgt gtgacatcaa ccttaagtat caacttttta
     5881 tcagacatag aacgtatgat ttactaagac tatttatgta taaaagttct aaataaatat
     5941 atatttatag agtcgcctgg cagtcatttg ggaaatataa catatatgat tagagaggca
     6001 tctatcgcaa aagaatgata atgatagagg tattgagcat atagatgagt ttaagttcat
     6061 cttgaaaata aagggttatt tagtcataga tgtagatgta taggaaatat ttgtatgtat
     6121 tgttcgatat gtatgaaatt ttcaataaaa gctaataacg cttatatgta actttcaaat
     6181 ttaaattata tacagagcat gatgattata aaaaaataac cacatcacat aaattgagtt
     6241 catacccaat ttaagtggtg tggctaataa tgttgattta tagatgaacc gcctaatcgt
     6301 taaacctctg ttacttcaac atcgatatgt tcaatacggt tgtatgcacc gtgatccaca
     6361 ggaccaacaa aatcattcat tttccaaccg tttttaatag cagaagcgac gaaagctttc
     6421 gcgctaatca cagcttcttt cggtgactta ccgttagcta aatatgcagt tgttgccgca
     6481 gcaaatgtac aaccagcacc atggttataa ctttgttgga acatgtctgt tgttagttga
     6541 taaaatgttt gaccatcata gtataagtca tacgatttat cttgatctaa agctttgcca
     6601 cctttaatga tgacatgctg tgcgccttta tcaaagataa ttgttgcagc ctttttcata
     6661 tcttcaattg aatttaattt acctaatcct gataattgac ccgcttcaaa taagtttggt
     6721 gtcactaccg ttgctttagg tagtaaatat ttaatcatcg cctcagtatt tccaggatta
     6781 agcacttcat cttcgccttt acaaaccatg acaggatcta ctacaaaata ttgtgcatta
     6841 gatgcctcat atacttctcc agcacgtttg attatctcct cagtacctaa catacctgtt
     6901 ttaatagcat caggtccgat tgataaagcc gtttcaagtt gtttttcaaa tacatccatt
     6961 ggtaatggtg taacatcgtg tgaccatgta tctttatcca tagtaacgat ggcagttaaa
     7021 gcgaccatgc catacgtatc taattcttgg aacgttttca aatctgcttg catacctgcg
     7081 ccagcacttg tgtcagaacc ggcaattgtt aaaactttct ttaaagccat tgagcttcac
     7141 tcctacataa taatattgta ttcatcatat catttttaac ctaattgaaa aatattaagc
     7201 attcaatatt tgatgattgt tgaaatgaat cattcatact attgtaactt ttgaaaatgt
     7261 cattcacttt agataagtgt gatatgttaa aatatgtcct gaggtgagat tgaatggaat
     7321 ggtcgcaaat ttttcatgac ataacaacga aacatgactt taaagctatg catgattttt
     7381 tagaaaaaga atattcgact gcaatcgtat accctgatag ggaaaatata tatcaagcgt
     7441 ttgatttaac accgtttgaa aatatcaaag ttgttatatt aggacaagac ccgtatcatg
     7501 gtccaaacca agcacatgga ttagcatttt cagtgcaacc taacgcaaaa ttccctccat
     7561 ctttacgtaa tatgtataaa gaattagcag atgatattgg atgcgttaga caaacaccgc
     7621 atttacaaga ttgggcaaga gaaggcgtct tgttattgaa tacagtttta accgtaagac
     7681 agggtgaagc aaattctcat cgtgatattg gttgggaaac atttactgat gaaattatta
     7741 aagcagtgtc tgattataaa gaacatgttg tctttatttt gtgggggaaa cctgcacagc
     7801 aaaaaataaa gcttatcgat acatctaaac attgtattat aaaatcagtg catcctagtc
     7861 cactgtctgc atatagagga ttctttggat caaaaccgta ttccaaagcg aatgcctatt
     7921 tagagtcagt aggaaaatca ccaattaatt ggtgtgaaag tgaggcgtag atgttgaata
     7981 gagaaacttt aatagcacga attgagcaag aattagtaca agcagagcag gcacagcatg
     8041 accatgactt tgaaaaacat atgtatgcca tacatatatt aacatcttta tatgcttcaa
     8101 catcaaatac accacatatt ggtgaacaac aaatgaatcg tcgtattgct aaccataatc
     8161 aaatgccaca atcacaaata acgcagccaa ctcatcaagt gacagttgct gaaattgaag
     8221 cgatgggtgg taaagtaaat acgcattcag cacatcatca taataagtca tattcacaac
     8281 cttcaaacca acaacaaaga ttagcgacag atgatgacat tggcaatggt gaatccatat
     8341 ttgattttta aaaagcaaca atgaaacata attacttaat agcttgttaa gtatgtaggt
     8401 taataatcaa gacgcatata cttttattcg agtgttcgga tttaaacatt tattaatact
     8461 gaattatata aggagaggta gcaatgaaat tatttattat tttaggtgca ttaaacgcga
     8521 tgatggctgt cggtacaggt gcatttggtg cgcatggttt acaaggaaaa ataagtgatc
     8581 actatttatc agtatgggaa aaagcaacga cgtatcaaat gtaccatggc ttagcattat
     8641 taattatagg tgtaattagt ggtacaactt caatcaatgt taactgggct ggctggttaa
     8701 tatttgctgg tattattttc tttagtggat cattatatat tttagtatta actcaaatta
     8761 aagttttagg tgcgattacg ccaattggtg gcgtattgtt catcattgga tggataatgt
     8821 taatcattgc gacattcaaa tttgctggtt aaattttaaa actttagatt acctatgtaa
     8881 ctaaacatta aatttttaat aaaaataatc aagaaaaaga gttacaaact catcttttgg
     8941 gtatagaata ccttcgaggt gagtttttat ttatggaaaa aaagaataag caaatagata
     9001 gaggcgattt aaaacaaaac ctatctgaaa agtttgtatg ggcgattgca tatggttcat
     9061 gtatcggatg gggcgcattc atcttaccag gagactggat taagcagtca ggtccgattg
     9121 cagcatcaat tggtatagtt attggtgcat tattaatgat attaattgcg gttagttatg
     9181 gcgcattagt agagagattt ccagtatcag ggggcgcgtt tgcctttagt ttcttaagtt
     9241 tcggcagata tgtgagtttc ttctcatcat ggtttttaac ttttggttat gtctgtgtcg
     9301 ttgctttaaa tgcgaccgca ttcagtttac tagttaaatt cttattgcca gatgtcttaa
     9361 ataatgggaa actatacacc attgcgggct gggacgttta tattacggaa atcattattg
     9421 cgaccgtatt actacttgta ttcatgctag taacgattcg tggcgcaagt gtatctggat
     9481 cattacaata ttatttctgt gtggcgatgg taatcgtcgt attattgatg ttctttggtt
     9541 cattctttgg taataatttt gcacttgaaa atttacaacc gttagctgaa cctagcaaag
     9601 gatggttagt gtctattgtg gttattgtat ccgtggcacc atgggcatat gttggatttg
     9661 ataatattcc acaaacagca gaagagttta actttgcacc aaacaagaca tttaagctta
     9721 tcgtgtacag tttattagca gcatcattaa cttatgttgt catgatttta tacactggtt
     9781 ggttatcaac aagtcatcaa agtttaaatg ggcagttgtg gttaacaggt gctgttacac
     9841 aaacagcatt tggttatatt ggattaggtg tattagcaat tgcaattatg atgggtatat
     9901 ttactggttt aaatggattc ttgatgagtt caagtcgctt gttattttct atgggacgtt
     9961 caggtattat gccaacaatg tttagtaaat tacatagtaa atacaaaaca ccatatgtcg
    10021 caatcatatt cctagtagga gtgtcgttaa ttgcaccttg gctaggaaga actgcattga
    10081 cttggattgt agatatgtca tctactggtg tatccattgc ctactttatt acatgtttgt
    10141 ctgcagcgaa attattcagt tataacaaac aaagtaatac gtatgcaccg gtttacaaaa
    10201 cgtttgctat tatcggctca tttgtatcat tcattttctt agcgttgtta ttagtgccag
    10261 gttctcctgc agcactgact gcaccgtctt atattgcatt acttggatgg ttaatcatcg
    10321 gtttaatatt ctttgtgatt cgatatccta aattgaaaaa tatggataat gatgaattaa
    10381 gtcgcttgat tttaaataga agtgaaaatg aagttgatga tatgattgaa gaacctgaaa
    10441 aagaaaaaac taaataataa aagaatcgca caataaacct tcttcattcg gaggcgtatc
    10501 gtgcgatttt ttgtattata aattgacatt taagacgagg cagctgaacc ttatatataa
    10561 ttgctaagag ttagggctga gccatttcta acaaatattt ataatcgttt aaaagatttc
    10621 acgaacccag aaacaattaa tttggaaatt tggtcggcga ataataaacc taatgcgatg
    10681 gcgcctgcaa taagtgtaac ctctagcatg gtattgattg ctgtactgaa atttaataag
    10741 actaaatttt ttgtagcatc gtatgctaag ccaccaggta ctaatggaat gatacccgtt
    10801 accataaaaa tgatggcagg ttctttttgt ttacgagcca tataatgact taacaagcct
    10861 aatgctaaac taccaaagaa actagagtat atagtgtgca cattaaagcc gttgaagaat
    10921 aaggtgtaaa ccatccatcc acacgtacca acgaaaccac atgatagata taattttcta
    10981 ggtgcatcaa aaatgacgca gaagaacatt gaagctaaaa agctaaagat aaagtttaag
    11041 atccaaaaca tagtctgata ctcctatact aaaattaata cgctaccaac gccagcaccg
    11101 atgccaaacg cagtaaccaa tgcttctaat gatttcgttg tgaacatcaa catgtgtcca
    11161 ccaaataaat cttgtattgc gtttgttatt aatacaccag gaacaatagg catgactgcc
    11221 gcaatgataa tagttgccaa gtcacctgtt ggaataagtg tatgtccaat aacggcgata
    11281 atcccaataa ctaatgaacc aatgaattct gggataaact gtgcgtgtaa cttacgatct
    11341 aaaatctcag tgactaggta tcctagacta cctgctaata tcgcagttaa aacatcaatc
    11401 aatctaccac cttgtaaata taagaaactc attgcaatca ttgctgcagc aaaaccttta
    11461 aagggaagac tgctgtcacg cttagcaaca tatatttttt caagttgcgt ttttgcttcg
    11521 gctaaagaaa tttcattgtt tgtaatttga cgcgaaattt tattagcttg agaaattttt
    11581 attaagtttg tatctcgaga ggtaattcta aatattctag gaaacgattc cgaatgtaac
    11641 gtaaactgga tgacagtgtt tgtaacaaag ctgttacttt cactgtaacc aagttttttt
    11701 gcaatacgtg tcatggtatc ttctacacgc gtaccttctg caccagattc taatagtatg
    11761 cgagcagcaa gcatgacaac gtctttgata agtacctctt gtttgtattc ttctgaattt
    11821 atgtccattt catcaccatt gtttataaga atttaatact cattatagtt tatacactat
    11881 aaaataacca catgagcttt ttgataagtt gttattgatt ttaaaaaact aatatttgat
    11941 actattgtaa taataatcat cggaat
//
LOCUS       DALAGD010000086        11964 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.087, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000086 DALAGD010000000
VERSION     DALAGD010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11964
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.087"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            29..157
                     /locus_tag="O2701_002109"
     CDS             29..157
                     /locus_tag="O2701_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293990.1"
                     /translation="MIKTMSIENMRTYQQRVNNLWIKGVDNVIYCGYQVGKQKIND"
     gene            complement(301..2229)
                     /gene="abc-f"
                     /locus_tag="O2701_002110"
     CDS             complement(301..2229)
                     /gene="abc-f"
                     /locus_tag="O2701_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HCZ4293991.1"
                     /translation="MILLQLNHISKSFDGEDIFTDVDFEVKTGERIGIVGRNGAGKST
                     LMKIIAGVENYDSGNVSKIKNLKLGYLTQQMTLNSNATVFEEMSKPFEHIKRMESLIK
                     EETDCLSKHANDYDSDTYKTHMSRYESLSNQFEQLEGYQYESKIKTVLYGLNFSEEDF
                     NKPINDFSGGQKTRLSLAQMLLNEPDLLLLDEPTNHLDLETTKWLEDYLRYFKGAIVI
                     ISHDRYFLDKIVTQIYDVALGDVKRYVGNYEEFIQQRDLYYQKRMQEYESQQAEIKRL
                     ETFVEKNITRASTSGMAKSRRKILEKMERIDKPMLDAKSANIQFGFDRNTGNDVMHVK
                     NLEIGYQTAITKPMSIEVSKGDHIAIIGPNGIGKSTLIKTIANQQKALNGDITFGANL
                     QIGYYDQKQAEFKSSKTILDYVWDQYPLMNEKDIRAVLGRFLFVQDDVKKIINDLSGG
                     EKARLLLALLMLQRDNVLILDEPTNHLDIDSKEMLEQALQHFEGTILFVSHDRYFINQ
                     LANKVFDLTIDGGKMYLGDYQYYIEKVEEAEALKAHQEEQSVNVQAHKKSMEQSSYHN
                     QKEQRREQRKLERQISECENEIETLETTILQIDEQLTQPEVYNNPQKANELAIQKQDS
                     EQKLEHAMSKWEELQQKL"
     gene            2482..3117
                     /locus_tag="O2701_002111"
     CDS             2482..3117
                     /locus_tag="O2701_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCZ4293992.1"
                     /translation="MSDQVKIPRATLKRLPLYYRFVSSLKSKGIDRVNSKAISDALQI
                     DSATIRRDFSYFGELGKKGYGYNIDSLLDFFKSELSESDMIKIAIVGVGNLGKALLTY
                     NFSIHDDMTITEAFDVKEDVIGQKIGNVIVKDNDELITTLKKEEIDVVILTTPERVAQ
                     KVADELVQAGVKGILNFTPGRINTPSDVQVHQIDLGIELQSLLFFMKNYSE"
     gene            3421..4500
                     /locus_tag="O2701_002112"
     CDS             3421..4500
                     /locus_tag="O2701_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HCZ4293993.1"
                     /translation="MWMIFSGLIVGGLLGFVMQRTRFCLTGGFRDMYVQKNNKMFYAL
                     LIAITIQSIGLLILTATDILQIPAHSFPILGTIIGSFIFGIGIVLAGGCATGTWYRAG
                     EGLIGSWIALVLYAVTAAITKTGILKPVMDKINQPTNVNSDMSQTTGIPFWGLVVILT
                     IITIFLVVRTLNNKKVRVAVPKLKQRYTGIRYYLFEKRYHPFIAAIVIGLIALLAWPM
                     SASTGRNDGLGITTPSANLVHFLITGETKFIDWGVFLVLGIFIGSYIAARGSREFKWR
                     LPDKITIRNSAIGGICMGFGASVAGGCSIGNGLVETATMTWQGWIALASMIVGVWTMS
                     HFIFVRPMKKVHQQSAKVKQQTQIV"
     gene            4560..4784
                     /locus_tag="O2701_002113"
     CDS             4560..4784
                     /locus_tag="O2701_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCZ4293994.1"
                     /translation="MIHELGTVGMVCPFPLIEAQKKMATLQSGDELKIDFDCTQATEA
                     IPNWAAENGYPVTNYEQIDNASWTITIQKV"
     gene            4992..6242
                     /locus_tag="O2701_002114"
     CDS             4992..6242
                     /locus_tag="O2701_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HCZ4293995.1"
                     /translation="MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSM
                     AAIVLVTFVWITVGFTISFGNGNLWFGNWEYTFLNHVGFATQEDISPHIPFALFMLFQ
                     MMFCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFA
                     GGTVVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFT
                     FDNIAMLAFTNTVISASAGAIGWLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVT
                     YLSATIMALIGGICCYIVINYIKVKLKYHDALDAFGIHGVGGIIGAVLTAVFQSKKAN
                     PDIENGFIYTGDIHIILVQILCVTAVVIFSIVMTFIIAKVIKLITPLSVTEQETNIGL
                     DKIVHGEHAYFEGELNRFNKHIRY"
     gene            6425..7375
                     /locus_tag="O2701_002115"
     CDS             6425..7375
                     /locus_tag="O2701_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCZ4293996.1"
                     /translation="MKNISDIAKLAGVSKSTVSRFLNNGSVSKKTSEKLTRIIAEHDY
                     QPNQFAQSLRARQTHLIGAIIPRMNSYAVDETIKGLAKQCQKYESQLILNYTGLNIEA
                     EIQALETLARSKVDGIVLMATDITERHIEVINKMNVPIVIVGQQHEQLHSIVHDDYKA
                     GQIIGEWIGQQGYQQVEVFSVSEKDIAVGIHRKRGLLDQLAKYQIKPNIHETNFTYVE
                     AQKDVANVLENVEQVDAVVGATDTIALAAYKYYSDKKDVMKPHQIYGFGGDPMTQLVS
                     PSIKTIHYNYFEAGQCAMEEIQQMLKKQDMPYSVTVDVNI"
     gene            7524..9008
                     /locus_tag="O2701_002116"
     CDS             7524..9008
                     /locus_tag="O2701_002116"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCZ4293997.1"
                     /translation="MTEWTREERYQRIEDVDTEYFKTLKQQVDQSKFRQQFHIQPETG
                     LLNDPNGLIFYKGKYYVSHQWFPLGAVHGLKYWYNYTSDDLINFKAEGPILNPDTKYD
                     SHGVYSGSAFEYNGHLYYMYTGNHRDNHWQRHASQMIARLKEDGSVEKFPKPVISQQP
                     EGYTSHFRDPKVFKYDEKYYAIIGAQNNDQQGRLLLYNTEDIINWHYLGEINTELDDF
                     GYMWECPDYFNLDNQDVILICPQGIEPKGDQFKNIYQSGYILGKFDIEKLTYEHENFV
                     ELDNGFDFYAPQTFLDEKGRRVLIGWMGLPEIEYPTDNEGWAHCLTIPRVLNVENGQL
                     KQRPYPALEKLRHNKETALGYANKFTRKLHPYEGKQYELIIDILDNDATEVYFELRTS
                     KTSSTLIAYNKRENKITLDRSDSGLLPTNVEGTTRSTILDTPLKQLQIFVDTSSIEIF
                     CNDGERVLTSRIFPTEDALGIKTSTESGQVYLQFTKYDLKDEHK"
     gene            9005..9964
                     /locus_tag="O2701_002117"
     CDS             9005..9964
                     /locus_tag="O2701_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCZ4293998.1"
                     /translation="MRRLFSIGEALIDFIPNVTNANLKDVQTFTKQIGGAPCNVACTV
                     QKLGQQAYMITQLGNDAFGDSIIETISSIGVDVSKVYRTNEANTALAFVSLTEAGERD
                     FSFYRKPSADMLFEPSFVNDIDVNENDVVHFCSVDLVDSPMRDAHYQLITKTLNANGT
                     VVFDPNVRLPLWDNAEDLRQTIHTFLPLAHIVKVSDEELEFITGIHDENEAIQSLFTG
                     NVTVVIYTKGADGAAVYLKNGINHYHSGYKVKPVDTTGAGDAFIGAVISRILATDVLN
                     LTQLFENEGEEILAFSNRVAAIVTTKYGAINSLPTLQEVEQAF"
     gene            complement(10152..10259)
                     /locus_tag="O2701_002118"
     CDS             complement(10152..10259)
                     /locus_tag="O2701_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4293999.1"
                     /translation="MHCIAGFLILLTKLNMTRELKNDVTSLCIPIPITT"
     gene            complement(10331..11047)
                     /locus_tag="O2701_002119"
     CDS             complement(10331..11047)
                     /locus_tag="O2701_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCZ4294000.1"
                     /translation="MKIFICEDDPKQRENMVTIIKNYIMIEEKPMEIALATDNPYEVL
                     EQAKNMNDIGCYFLDIQLSTDINGIKLGSEIRKHDPVGNIIFVTSHSELTYLTFVYKV
                     AAMDFIFKDDPAELRTRIIDCLETAHTRLQLLSKDNSVETIELKRGSNSVYVQYDDIM
                     FFESSTKSHRLIAHLDNRQIEFYGNLKELSQLDDRFFRCHNSFVVNRHNIESIDSKER
                     IVYFKNKEHCYASVRNVKKI"
     gene            complement(11066..>11964)
                     /locus_tag="O2701_002120"
     CDS             complement(11066..>11964)
                     /locus_tag="O2701_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCZ4294001.1"
                     /translation="NRVLKKISTPYLILNKGFLIVISTILLLTFSLFFFYSQINSDEA
                     KVIRQYSFIFIGITIFLSILTFVISQFLLKEMKYKRNQEEIETYYEYTLKIEAINNEM
                     RKFRHDYVNILTTLSEYIREDDMPGLRDYFNKNIVPMKDNLQMNAIKLNGIENLKVRE
                     IKGLITAKILRAQEMNFPISIEIPDEVSSINLNMIDLSRSIGIILDNAIEASTEIDDP
                     IIRVAFIESENSVTFIVMNKCADDIPRIHELFQESFSTKGEGRGLGLSTLKEIADNAD
                     NVLLDTIIENGFFIQKVEIINN"
BASE COUNT         4042 a         1692 c         2126 g         4104 t
ORIGIN      
        1 cataaagaaa atgtcttaac aatgaatttt gataaaaaca atgtcaatag agaacatgcg
       61 tacttatcaa caaagagtga ataacttgtg gataaaaggt gttgataacg tcatttattg
      121 tggatatcag gtgggaaaac agaaaataaa tgattgatta gtgttaattt acttgtgggt
      181 aactaggata agtctgtgga tagctataat atcagcgttt ttattgtgaa taaaaaaatt
      241 aaaatttgta aaaaaataac tctaactata gcgattttac agttagagtt atgtatattt
      301 ttatagtttt tgttgtaatt cttcccattt agacatagcg tgttctaatt tttgttcgct
      361 atcttgtttt tggatagcta attcgtttgc tttttgtgga ttgttataca cttctggttg
      421 ggttaattgc tcgtctattt gtaagatagt agtctctaaa gtttctattt cattttcaca
      481 ctcggatatt tgtcgttcaa gtttgcgttg ttcacgtctt tgttcttttt ggttatgata
      541 cgacgattgt tccatagact ttttatgtgc ttgtacgtta actgactgtt cttcttggtg
      601 cgctttcaac gcttccgctt cctcaacttt ttcaatgtaa tattgataat ctcctaaata
      661 catctttccg ccatcaattg ttaaatcaaa taccttgttt gctaattggt taataaagta
      721 acgatcatgg gaaacaaata gtattgttcc ttcaaaatgt tggagtgctt gctctaacat
      781 ttcttttgaa tctatatcga ggtggttggt aggttcatct aaaataagta cattgtcacg
      841 ttgcaacata agtagtgcta gtagtaaacg tgctttttca ccaccagata aatcattaat
      901 tatcttttta acatcgtctt gtacaaataa gaaacgtcca agaactgctc gaatatcttt
      961 ttcattcatt aacggatatt gatcccacac ataatctaaa atcgttttac tagatttaaa
     1021 ttctgcttgc ttttgatcat aataaccaat ttgtaaattt gcgccgaaag taatatcgcc
     1081 attaagcgct ttttgttgat tagcaatagt tttaattaag gtcgattttc caataccatt
     1141 tggcccaatg attgctatat gatcgccttt agagacctct atactcatag gtttggtaat
     1201 tgcagtttga taaccgattt ctaaattttt tacatgcatg acgtcattac ctgtattccg
     1261 gtcaaagcca aattgaatat ttgcactttt ggcatctaac attggtttat caatgcgttc
     1321 cattttttct aaaatcttac gtctactttt tgccattcca cttgttgaag cacgggtaat
     1381 atttttctca acaaaagttt ctaatcgttt tatttctgct tgttgacttt catattcttg
     1441 cattcgtttt tgataatata aatcccgttg ctgtataaat tcctcgtaat taccaacata
     1501 gcgtttgaca tcacctaaag ccacatcata aatttgagta actattttat ctaaaaagta
     1561 acgatcatgg ctgatgatga cgattgcacc tttaaaataa cgtagataat cttcaagcca
     1621 ctttgtcgtt tctaaatcta agtggttagt aggttcatct aaaagtaata aatcaggttc
     1681 gtttaatagc atttgagcta aagataaacg tgttttttgg ccaccgctaa aatcattgat
     1741 aggtttattg aaatcttctt cactaaaatt taacccgtaa agtactgttt taattttact
     1801 ttcatattga tatccttcta attgttcaaa ttgattcgat aaagattcat aacgggacat
     1861 atgtgtttta tatgtatcac tatcgtaatc atttgcatgt tttgataaac aatctgtttc
     1921 ttctttgatt aagctttcca ttcgtttaat atgttcaaat ggttttgaca tttcttcaaa
     1981 aactgttgca ttagaattta aagtcatttg ttgagttaaa taacctagtt ttaagttttt
     2041 aatttttgac acatttccag aatcatagtt ttctacgcca gctataattt tcatcaatgt
     2101 tgatttaccg gcgccatttc ttcctacgat accaattcgt tcaccagttt ttacttcaaa
     2161 atcaacatca gtaaatatat cttcaccatc gaacgatttt gatatatgat tgagttgtaa
     2221 aagtatcatt cgatcttcac ctttcgtctt tctcaaattt tattaactta tgaaaaaagt
     2281 cgtaattatt acattaataa ccattttaca gtataaaacg ccgtcttgaa actaagatat
     2341 ttatttttaa aattcaataa tgtataatat ttaggtattc attgtaatca attatttgtt
     2401 ttttaataat ctaacaatta cgctctaact cgtttgaata catagatata attcattcaa
     2461 acacgaagga ggaaatagcg aatgagtgac caagttaaaa ttcctcgagc aactttaaaa
     2521 cgtttgccgt tatattatag atttgtcagt tcattaaaat ctaaaggtat agatcgtgta
     2581 aattcaaaag cgattagcga tgcgttacaa attgactcgg caacaattcg tcgtgacttt
     2641 tcatattttg gcgaattagg taaaaaaggg tacggatata atatagatag tttattggat
     2701 ttctttaaat ctgaactaag cgagagtgac atgatcaaaa tcgcaattgt cggagttggg
     2761 aacctaggga aagctttgct cacatataac ttttcaatac atgacgatat gacgattaca
     2821 gaagcgtttg acgtaaaaga agatgttatt ggccagaaaa tagggaacgt tattgttaaa
     2881 gataacgatg aattaataac aacattgaag aaggaagaaa tagatgttgt gattctaact
     2941 acaccagaaa gagttgcaca gaaagttgca gatgaactcg tccaagctgg tgtgaaaggt
     3001 attttaaact tcactcctgg tagaattaat acgccttcag atgtgcaagt acatcaaatt
     3061 gacttaggta tagaattaca gtcattatta ttctttatga aaaattacag tgaataaatg
     3121 tgatgttagc tttgaatgat taaccttgtt atatctttaa ttaattatag aacagtctgg
     3181 gactaagtat gtctcaggct ttttctgtta tgtttgaaaa tgaaaggaaa aactgttgtg
     3241 ttgataaata tagtataggt taataaatta acacgtagag atataatgat gaatcgtaat
     3301 taatcgcctt ccttatatga atataatata aatatcaaaa atattgtgta taaggttgaa
     3361 aacaagaaaa tcatcaccta taatacatac taaacttatc ggaaatgaga ggttaagatt
     3421 atgtggatga tttttagtgg cttaattgtc gggggactct taggttttgt catgcaaaga
     3481 acaagatttt gtttaacagg tggctttcga gatatgtatg tgcaaaagaa taataagatg
     3541 ttctatgcat tattaatcgc tattactatt caaagtatag gattattgat tttgacggca
     3601 acagatattt tacaaattcc tgcacatagt tttccaatat tgggaacaat tataggttct
     3661 tttatttttg gaattggaat agtattggct ggaggatgtg caacaggtac ttggtatcgc
     3721 gctggtgaag ggctaattgg tagttggatt gcattagtat tatatgctgt tactgcagca
     3781 atcactaaaa cagggatttt aaagccagta atggataaaa ttaatcaacc aacgaatgta
     3841 aatagtgata tgtctcaaac aactggcatt ccgttttggg gattagtcgt tatattaact
     3901 ataatcacca tttttctagt tgtaagaaca ctcaataata agaaagttcg agttgctgtt
     3961 ccaaaactaa agcaacgtta tacaggtatt agatattacc ttttcgaaaa acgataccat
     4021 ccatttattg cagcaattgt aattggactt atcgcactct tagcttggcc aatgagtgca
     4081 tcaactggaa gaaatgacgg tttaggtata acaacgcctt cagcaaattt agtacacttt
     4141 ttgattacag gtgaaactaa atttattgat tggggtgtct ttttagttct aggaattttc
     4201 attggttcat atattgcagc tagaggatca agagaattta aatggcgatt gccagacaag
     4261 attacaatac gaaacagtgc cattggtggc atatgtatgg gatttggtgc gtcagttgct
     4321 ggtggttgtt ctatcggtaa cggtttggtt gaaacggcaa cgatgacttg gcaaggatgg
     4381 attgcgctag catcgatgat agttggtgta tggacaatga gtcattttat ctttgttcgt
     4441 ccaatgaaaa aagtacacca acaatctgca aaggttaaac agcaaacgca aatagtatag
     4501 aagattatta tgcaaatgat gttgatcaaa taaaagtgat tggaaaagga gaaataatta
     4561 tgatacacga attaggtaca gtaggaatgg tatgtccatt tccgttaatt gaagcgcaaa
     4621 agaaaatggc aacattgcaa tctggagatg aattaaaaat tgattttgat tgcacgcaag
     4681 cgacggaagc cattccaaat tgggctgcag aaaatggtta tcctgtaaca aactatgaac
     4741 aaattgataa tgcttcatgg acaattacaa ttcaaaaagt ttaacgttat cattttaaca
     4801 ataaaataga tattagattc tatggctact tccgctaatt taaaagtgag taagtagtct
     4861 tttttgtttt agttcatgaa atcattttta tatagtgtgg cacattttat tccaaaagat
     4921 gtaataaaac ttaacgcatt tttgcttttt ataaattgtc agattattat gaaaaaaagg
     4981 gagtggtaag tatgaatctt aacgatacga tatttatgtt tttgtgtaca ttattagttt
     5041 ggttaatgac accaggatta agtttatttt atggtgggtt agttcaatct aaaaatgcgc
     5101 ttaatactgt catgcaaagt atggcagcaa ttgtgcttgt tacatttgta tggataacag
     5161 ttggttttac aattagtttt gggaatggga atttatggtt cggaaattgg gaatatactt
     5221 ttcttaatca tgtaggtttt gcgactcaag aagatattag cccacatatt cctttcgctt
     5281 tgtttatgtt atttcaaatg atgttttgta cgattgcaat ttctatttta tctggttcaa
     5341 tcgctgagaa aatgaagttt attccttatt tattattcgt agtaatatgg actgctcttg
     5401 tatacagtcc agtagcacat tgggtttggg gcggcggttg gattaacaaa ctcggtgtat
     5461 tagatttcgc tggaggtacg gttgttcata ttacatcagg tgtttctggt ttagtattag
     5521 ctattatgat tggaaaagga aacaaacatt ctgaatcaac accacataat cttatcatta
     5581 cgttgattgg cggtatattc gtgtggattg gttggtatgg atttaatgta ggtagtgctt
     5641 ttacatttga taatattgcg atgcttgcat ttacaaatac tgtcatttca gccagtgcag
     5701 gtgctatagg ttggttaatt ttagaatata tttttaaaaa gacgacaagt ttacttggac
     5761 ttttactcgg tgcattagca ggattagttg tcattactcc tgcagcagga tatgtaacat
     5821 atcttagtgc aacaataatg gctttaatag gaggtatctg ttgttatatt gtcattaatt
     5881 acatcaaggt aaaactaaaa tatcatgatg cattagatgc atttggtatt catggtgttg
     5941 gtggtattat tggtgctgtt ttaacagcag ttttccaaag taaaaaagcc aatcctgaca
     6001 ttgagaatgg ctttatttat actggtgaca tacatattat acttgtacaa atattatgtg
     6061 taacagcagt tgtaattttt agtatagtca tgacgtttat tattgcgaaa gtaattaaat
     6121 taattacacc attatctgtt acggaacaag aaacgaatat aggattagac aagattgttc
     6181 acggtgaaca tgcttacttt gaaggtgagc taaatagatt caataaacat attcgatatt
     6241 agaatatatt tacatagaat attcattgtc ctgacattta actaaaggtt gatgttggga
     6301 cattttgtta tacaaaagtt ttattttgaa atctttttat gaaagaagca gaaatattat
     6361 ttaaagcggt tacacatatg ctaaaataag gctaagtgtc acaaataatg ataggtgaat
     6421 aagtatgaaa aatatatctg atattgccaa attggcaggc gtttcaaaaa gtacagtatc
     6481 tagattttta aataatggat ctgtcagtaa aaaaacaagt gaaaaattaa caagaattat
     6541 agcagaacat gactatcaac cgaatcaatt tgctcaaagt ttaagagcga gacaaacaca
     6601 ccttattggt gccattatac ctagaatgaa ttcatatgca gtagatgaaa caatcaaagg
     6661 attggcaaaa caatgccaaa aatatgaatc acaattaatt ttaaattaca caggtttaaa
     6721 tatcgaagca gaaatacaag cgcttgaaac attagcacgc agtaaagtag atggtattgt
     6781 tttaatggct acagacataa cagagagaca tattgaagtc attaataaaa tgaatgtacc
     6841 aatcgttatt gttggtcaac aacatgaaca acttcatagt attgtgcatg atgattataa
     6901 agcaggtcaa attataggcg aatggattgg tcaacaggga tatcaacaag ttgaagtgtt
     6961 tagtgtaagt gaaaaagata ttgcagttgg tatacataga aaacgtggtt tacttgacca
     7021 gttagctaaa taccaaatta aacctaatat acatgaaaca aattttactt atgtggaagc
     7081 acaaaaagat gttgcaaatg ttttggaaaa tgtggagcaa gtagatgcgg ttgttggagc
     7141 aactgatacg attgcattag ctgcctataa atattattct gataaaaaag atgttatgaa
     7201 accacatcaa atatatggtt ttggtggtga cccaatgaca caattagtgt ctccatcgat
     7261 aaaaacaatt cattataatt attttgaagc tggccaatgc gcgatggaag agatacaaca
     7321 gatgcttaaa aagcaagata tgccatatag cgtcacagta gatgttaata tttagacgct
     7381 gtatttttta aaataaatgt ggaaccgata ccatataact ataaatggat aggttaaaag
     7441 ttaaagaacg taggtaaaat ttgctataat agaatataaa ttgttattag cataaattat
     7501 aaaaggagga ctgggtaaat attatgaccg aatggactag agaagaacgt tatcaacgaa
     7561 tcgaggacgt tgatactgag tattttaaaa cattaaaaca acaagttgat caatcaaaat
     7621 ttcgtcaaca atttcatata caaccagaaa caggcttatt aaatgacccc aacggactta
     7681 ttttttataa agggaagtat tatgtttcac atcaatggtt cccattaggc gcagtacatg
     7741 gcttaaagta ttggtataac tacacgagtg atgacttaat aaactttaaa gctgaagggc
     7801 caattttaaa tccagatact aaatatgaca gccatggtgt atatagcggt agcgcttttg
     7861 aatataacgg gcatttatat tatatgtaca caggaaatca tcgagataat cattggcaac
     7921 gacatgcgag tcagatgatc gcacgattga aagaagacgg ttcagttgaa aagtttccaa
     7981 agccagtaat tagccaacaa ccagaaggat atacgagtca ttttagagat cctaaagttt
     8041 ttaaatatga tgagaaatat tatgcaatca ttggtgcaca aaataatgat cagcaaggtc
     8101 gattattact ttataatact gaagatataa ttaattggca ttatttaggt gaaataaata
     8161 cagagttgga tgattttgga tatatgtggg aatgcccaga ttactttaat ttagataatc
     8221 aagatgtcat acttatttgt ccacaaggta ttgaaccaaa aggcgatcag ttcaaaaata
     8281 tttatcaaag tggttatata cttggaaagt ttgatattga aaagttaaca tatgaacatg
     8341 aaaattttgt cgagcttgat aatggttttg atttctatgc acctcaaaca tttttagatg
     8401 aaaaaggccg acgagtacta attggatgga tggggttacc ggaaatcgaa tatcctactg
     8461 ataatgaagg atgggcccat tgcttaacaa ttcctcgtgt attaaatgta gaaaatggtc
     8521 aacttaagca acgtccatat ccagcgttgg aaaaattacg tcacaataaa gagacagcat
     8581 taggctacgc aaataaattt actcgaaaat tacatccgta tgaaggtaaa cagtatgaat
     8641 taatcataga tattttggat aatgatgcta ccgaagtgta ctttgaatta cgtacatcta
     8701 agacttcttc aacattaatt gcttataaca agcgtgaaaa taaaataaca ttagatcgca
     8761 gcgacagtgg tttattgccg acaaatgttg aaggtacgac gcgtagtacg atattagaca
     8821 cgccattaaa gcaattacaa atttttgttg atacatctag tatagaaatt ttctgtaatg
     8881 atggagaacg tgttttgaca tctcgaattt tcccgacaga agacgcacta ggtattaaaa
     8941 catcaacaga atcagggcaa gtatatttac aatttactaa atatgattta aaggatgaac
     9001 ataaatgaga cgtctatttt caataggaga agcgttaatt gattttattc caaatgttac
     9061 aaatgcgaat ttaaaagatg ttcaaacatt tacgaagcaa attggtggcg cgccatgcaa
     9121 tgtagcttgt acagttcaaa agttaggaca acaagcgtat atgattacac aattaggtaa
     9181 tgatgcattt ggagatagca ttattgaaac gatatcaagt attggtgtag atgtatcaaa
     9241 agtgtatcgc acgaatgaag caaacactgc gttagctttt gtgagtttga ctgaagcggg
     9301 cgagcgagat ttttcttttt atcgtaagcc ctctgctgat atgttatttg aaccaagttt
     9361 tgtgaatgat attgatgtga atgaaaatga tgtagtacat ttttgttctg ttgatttggt
     9421 cgatagtccg atgagagatg cacattatca attgattacc aaaacattaa atgcaaatgg
     9481 tactgtcgta tttgatccta atgtaagatt gccattatgg gataacgctg aagatttaag
     9541 acaaactatt catacctttt tgccattggc gcatattgta aaagtttcag atgaagaatt
     9601 agaatttatt acaggtattc atgatgaaaa tgaagccatt caatcattat ttacaggtaa
     9661 tgttactgta gtcatttata cgaagggagc agatggtgct gccgtatatt tgaaaaatgg
     9721 tattaatcat tatcatagtg gctataaggt aaaacctgtt gatacaactg gggcagggga
     9781 tgcatttatt ggtgcagtta ttagcaggat tttagcaacc gatgtgttaa atttaactca
     9841 gttatttgaa aatgaaggtg aggaaatttt agcgtttagc aatcgcgttg ctgcaatagt
     9901 gacaacgaag tatggtgcta ttaatagttt gccaacatta caagaggttg aacaagcatt
     9961 ttaaataatt catttataaa acagtgtagg aaacaagttg aatcttgtgc tacactgttt
    10021 tttaaaacaa aataagattc acggagtagg aaattatagc attttattta tggaaactgt
    10081 caatttcaaa tttagtatat tttgttaaaa attgcgccat aggattgtag agtgaaatag
    10141 aaattgataa attatgtagt aataggaata ggtatacaaa gagacgttac atcatttttt
    10201 aattcacgcg tcatatttaa ttttgttaat aaaattaaaa agccagctat acagtgcatt
    10261 tgctagttat cttgttaaaa tccaacaaga tttacaattg aatacgccgt taactgactt
    10321 tattatctta ttatattttt ttaacgtttc tcaccgatgc atagcagtgt tctttatttt
    10381 taaaatagac aattcgctct ttcgaatcta tagattcaat attatggcga ttgacgacaa
    10441 agctattatg acatctaaag aaacgatcat ctaattgact cagttctttt aaattaccat
    10501 aaaattcaat ttgacggtta tctaaatggg caatgagtct gtgagatttt gttgatgatt
    10561 caaaaaacat aatatcatca tattgaacat acactgaatt actgccacgt tttaattcaa
    10621 tcgtttcaac gctattatct ttagacaaca attgtaagcg tgtatgtgca gtttctaaac
    10681 agtctataat tcgagttctt aattcagctg gatcatcttt aaaaataaaa tccatcgctg
    10741 caactttgta gacaaatgtt aaataggtaa gttcactgtg actcgtaacg aaaataatgt
    10801 taccaactgg gtcatgctta cgaatttcac tgcctaattt gataccatta atatcagttg
    10861 aaagttgaat atctaaaaag taacagccta tgtcattcat atttttagct tgctcaagca
    10921 cctcataagg attatcagtt gcgagggcaa tttccatagg cttttcttct atcattatat
    10981 aatttttaat aatggtaacc atgttttctc tttgttttgg atcgtcttcg caaatgaaaa
    11041 ttttcataca ttcacatcct tatggctagt tgttaataat ttcaactttt tgaataaaga
    11101 aaccattttc gataattgta tctaataaga cattgtctgc attatcagca atttctttta
    11161 aagttgatag acctaaacca cgaccttcac ctttagtaga aaaactttct tggaacaatt
    11221 catgaatgcg tggtatatca tcagcgcatt tattcataac aataaacgtt actgaatttt
    11281 cactttcaat aaatgcaacg cgaatgatag ggtcatcaat ttcagttgat gcctcaattg
    11341 cattatcaag aataatacca atactgcgac ttaaatcgat catattcaag ttaatgctac
    11401 ttacttcatc gggtatttcg atactaatcg gaaaattcat ttcttgtgca cgtaaaattt
    11461 tcgcagtaat taagccttta atttcacgta ctttaagatt ctcgatacca tttaatttta
    11521 tagcattcat ttgtaaattg tctttcatag gtacaatatt tttattgaaa taatcacgta
    11581 ggccaggcat gtcatcttct cgaatgtatt ctgaaagtgt cgttaagata ttgacataat
    11641 catgacggaa cttgcgcatt tcgttgttga tagcttcaat cttcaatgta tattcataat
    11701 aggtttcaat ttcttcttga ttacgtttat atttcatctc tttaaggaga aattgagaaa
    11761 taacaaatgt taatatactt aaaaatatag tgataccaat aaaaataaaa gaatactgcc
    11821 ttattacttt agcttcatcc gagtttattt gtgaataaaa gaaaaataat gaaaaagtaa
    11881 gcagtaagat agtcgaaata actattaaaa atcctttgtt tagtattaga tatggtgtgc
    11941 taattttttt gagaactcta ttta
//
LOCUS       DALAGD010000087        11742 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.088, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000087 DALAGD010000000
VERSION     DALAGD010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11742
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.088"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            319..3024
                     /gene="acnA"
                     /locus_tag="O2701_002121"
     CDS             319..3024
                     /gene="acnA"
                     /locus_tag="O2701_002121"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCZ4294002.1"
                     /translation="MAANFKEQSKKHFDLNGQSYTYYDLKAVEEQGITKVSNLPYSIR
                     VLLESLLRQEDDFVITDDHIKALSQFGKDGNEGEVPFKPSRVILQDFTGVPAVVDLAS
                     LRKAMDDVGGDITKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFL
                     NWATKAFDNYNAVPPATGIVHQVNLEYLASVVHVRDVDGEKTAFPDTLVGTDSHTTMI
                     NGIGVLGWGVGGIEAEAGMLGQPSYFPIPEVIGVRLVNSLPQGATATDLALRVTQELR
                     KKGVVGKFVEFFGPGVQHLPLADRATIANMAPEYGATCGFFPVDDESLKYMKLTGRSD
                     EHIALVKEYLKQNHMFFDVEKEDPNYTDVIELDLSTVEASLSGPKRPQDLIFLSDMKS
                     SFENSVTAPAGNQGHGLDKSEFDKKAEINFKDGSKATMKTGDIAIAAITSCTNTSNPY
                     VMLGAGLVAKKAVEKGLKVPEYVKTSLAPGSKVVTGYLRDAGLQPYLDDLGFNLVGYG
                     CTTCIGNSGPLLPEIEKAIADEDLLVTSVLSGNRNFEGRIHPLVKANYLASPQLVVAY
                     ALAGTVDIDLQNEPIGKGNDGEDVYLKDIWPSIKEVSDTVDSVVTPELFIEEYNNVYN
                     NNELWNEIDVTDQPLYDFDPNSTYIQNPSFFQGLSKEPGTIVPLNGLRVMGKFGDSVT
                     TDHISPAGAIGKDTPAGKYLQDHQVPIREFNSYGSRRGNHEVMVRGTFANIRIKNQLA
                     PGTEGGFTTYWPTNEVMPIFDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLG
                     VKTVIAQSYERIHRSNLVMMGVLPLEFKKGESADSLGLDGTEEISVNIDENVQPHDYV
                     KVTAKKQDGDLVEFDAMVRFDSLVEMDYYRHGGILQMVLRNKLAQ"
     gene            3204..3671
                     /locus_tag="O2701_002122"
     CDS             3204..3671
                     /locus_tag="O2701_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCZ4294003.1"
                     /translation="MIYSITEIEARYAETDKMGVIYHGNYATWFEVARLDYISKLGFS
                     YADMEKQGIISPVTDLNVNYKKSIFYPEKVKVKTWVEKYSRLRSVYKYEIFNEKGELA
                     TTGSTELICIKEDTFKPIRLDRYFPDWHEAYSKVQTLNNEGKTVEIMDGIDSL"
     gene            complement(3878..4174)
                     /locus_tag="O2701_002123"
     CDS             complement(3878..4174)
                     /locus_tag="O2701_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HCZ4294004.1"
                     /translation="MQIELTDAAVTWFKNELELPENNKVLVFFVRYGGEFQLKQGFSP
                     AFTVEPKEDVDIGYEQQYDDLNVVVAEKDLWYFEDDHIIVNVVDHEDEISYSTK"
     gene            complement(4550..5158)
                     /gene="plsY"
                     /locus_tag="O2701_002124"
     CDS             complement(4550..5158)
                     /gene="plsY"
                     /locus_tag="O2701_002124"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCZ4294005.1"
                     /translation="MMIIVMLLLSYLIGAFPSGFVIGKLFFKKDIRQFGSGNTGATNS
                     FRVLGRPAGFLVTFLDIFKGFITVFFPLWLQVHADGPISTFFTNGLIVGLFAILGHVY
                     PVYLKFQGGKAVATSAGVVLGVNPILLLILAIIFFIVLKIFKYVSLASIVAAICCVIG
                     SLIIQDYILLVVSFLVSIILIIRHRSNIARIFRGEEPKIKWM"
     gene            5364..7355
                     /gene="parE"
                     /locus_tag="O2701_002125"
     CDS             5364..7355
                     /gene="parE"
                     /locus_tag="O2701_002125"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCZ4294006.1"
                     /translation="MNKQNNYSDDSIQVLEGLEAVRKRPGMYIGSTDKRGLHHLVYEI
                     VDNSVDEVLNGYGNEIDVTINKDGSISIEDNGRGMPTGIHKSGKPTVEVIFTVLHAGG
                     KFGQGGYKTSGGLHGVGASVVNALSEWLEVEIHRDGNIYHQSFKNGGSPSSGLVKKGK
                     TKKTGTKVTFKPDDTIFKASTSFNFDVLSERLQESAFLLKNLKITLNDLRSGKERQEH
                     YHYEEGIKEFVSYVNEGKEVLHDVATFSGEANGIEVDVAFQYNDQYSESILSFVNNVR
                     TKDGGTHEVGFKTAMTRVFNDYARRINELKTKDKNLDGNDIREGLTAVVSVRIPEELL
                     QFEGQTKSKLGTSEARSAVDSVVADKLPFYLEEKGQLSKSLVKKAIKAQQAREAARKA
                     REDARSGKKNKRKDTLLSGKLTPAQSKNTEKNELYLVEGDSAGGSAKLGRDRKFQAIL
                     PLRGKVINTEKARLEDIFKNEEINTIIHTIGAGVGTDFKIEDSNYNRVIIMTDADTDG
                     AHIQVLLLTFFFKYMKPLVQAGRVFIALPPLYKLEKGKGKTKRVEYAWTDEELNKLQK
                     ELGKGFTLQRYKGLGEMNPEQLWETTMNPETRTLIRVQVEDEVRSSKRVTTLMGDKVQ
                     PRREWIEKHVEFGMQEDQSILDNSEVQVLENDQFDEEEI"
     gene            7355..9757
                     /gene="parC"
                     /locus_tag="O2701_002126"
     CDS             7355..9757
                     /gene="parC"
                     /locus_tag="O2701_002126"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCZ4294007.1"
                     /translation="MSEIIQDLSLEDVLGDRFGRYSKYIIQERALPDVRDGLKPVQRR
                     ILYAMYSSGNTHDKNFRKSAKTVGDVIGQYHPHGDYSVYEAMVRLSQDWKLRHVLIEM
                     HGNNGSIDNDPPAAMRYTEAKLSLLAEELLRDINKETVSFIPNYDDTTLEPMVLPSRF
                     PNLLVNGSTGISAGYATDIPPHNLAEVIQATLKYIDNPDITVNQLMKYIKGPDFPTGG
                     IIQGIDGIKKAYESGKGRIIVRSKVEEETLRNGRKQLIITEIPYEVNKSSLVKRIDEL
                     RADKKVDGIVEVRDETDRTGLRIAIELKKDVNSESIKNYLYKNSDLQISYNFNMVAIS
                     DGRPKLMGIRQIIDSYLNHQIEVVANRTKFELDNAEKRMHIVEGLIKALSILDKVIEL
                     IRSSKNKRDAKENLIEVYEFTEEQAEAIVMLQLYRLTNTDIVALEGEHKELEALIKQL
                     RHILDNHDALLNVIKEELNEIKKKFKSERLSLIEAEIEEIKIDKEVMVPSEEVILSMT
                     RHGYIKRTSIRSFNASGVEDIGLKDGDSLLKHQEVNTQDTVLVFTNKGRYLFIPVHKL
                     ADIRWKELGQHVSQIVPIEEDEVVINVFNEKDFNTDAFYVFATQNGMIKKSTVPLFKT
                     TRFNKPLIATKVKENDDLISVMRFEKDQLITVITNKGMSLTYNTSELSDTGLRAAGVK
                     SINLKAEDFVVMTEGVSENDTILMATQRGSLKRISFKILQVAKRAQRGITLLKELKKN
                     PHRIVAAHVVTGEHSQYTLYSKSNEEHGLINDIHKSEQYTNGSFIVDTDDFGEVIDMY
                     IS"
     gene            10007..11467
                     /locus_tag="O2701_002127"
     CDS             10007..11467
                     /locus_tag="O2701_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HCZ4294008.1"
                     /translation="MKDFDSLIPGWFKEFVHVGTDLIWSQYLIGLLLTAGFFFTISSK
                     FVQLRMLPEMFRALVERPETLEDGKKGISPFQAFAISAGSRVGTGNIAGVATAIVLGG
                     PGAVFWMWVIAFIGAASAFIEATLAQVYKVHDKDGGFRGGPAYYITKGLNQKWLGIVF
                     AILITITFAFVFNTVQSNTIAESLNTQYNISPVITGIILAIVTAIIIFGGVRSIATLS
                     SLIVPIMAIIYIGMVLVILLFNLDQIVPMIGTIIKSAFGIEQVTGGAVGAAVLQGIKR
                     GLFSNEAGMGSAPNAAATAAVPHPVKQGLIQSLGVFFDTMLVCTATAIMILLYSGLKF
                     GDNAPQGVAVTQSALNEHLGSAGGIFLTIAVTLFAFSSVVGNYYYGQSNMEFLSTNRV
                     ILFIFRCLVVVLVFVGAVVKTETVWNTADLFMGLMAIVNIISIIGLSNVAFALMKDYQ
                     KQKKEGKNPVFKPENLEINLFGISAWGANKYKNSDK"
BASE COUNT         4101 a         1687 c         2234 g         3720 t
ORIGIN      
        1 atttctgtcc cactcccatc aaaagaatga cattgaaaaa aatattaaag tttggtgcca
       61 taattttctt tctgctcaat gaaataatac gtttaaaaat tgaattgaat cattcgcgaa
      121 attgataatt attctcaatt aataaaaaat acttattaca ccttacctgt atgataagtt
      181 ttgcttatat actctgatta aaaagtcaaa acctaaatta agcgttttct aagcatgatg
      241 agttgtttat caataaatat aataatacaa tttaatcaag gcatataaat ataaaaatgt
      301 atcaaggggg atcattaaat ggctgcaaat tttaaagagc aatcaaaaaa acattttgac
      361 ttgaatggcc aaagttatac ttactatgat ttaaaagctg tagaagagca aggtattact
      421 aaagtttcca atttacctta ttcaattcgt gttttgttag aatctttact tcgtcaagaa
      481 gatgattttg taattacaga cgatcatatt aaagctttaa gtcagtttgg aaaagatgga
      541 aatgaaggcg aggtgccatt taaaccttct cgtgttattt tacaagattt cacaggtgta
      601 ccagccgtag ttgatttagc ttctttacgt aaagcaatgg atgacgttgg gggagatatt
      661 actaaaatta atccagaagt accggtggat ttagttattg accactcagt tcaagtggat
      721 agctatgcaa atccagaagc tcttgaacgt aatatgaaat tagaatttga acgtaactat
      781 gaacgttatc agtttttaaa ttgggcaacg aaagcatttg ataattacaa tgcagttcct
      841 cctgcaactg gaatagttca ccaagttaac ttagaatatt tagcaagtgt tgtacatgtt
      901 cgtgatgtag atggtgaaaa aactgcattt ccagatacat tagttggtac tgattcacat
      961 acaacaatga taaatggtat tggcgtacta ggatggggtg ttggtggtat tgaagctgaa
     1021 gctggaatgc ttggacaacc ttcttatttc ccaattccag aggttattgg tgtacgacta
     1081 gtaaattcat taccacaagg cgcaacagca actgatttag cgttaagagt aactcaagag
     1141 ctacgtaaaa aaggtgttgt tggtaaattt gtggagttct ttggtccagg tgtacaacat
     1201 ttaccactag cagaccgtgc tacaattgca aacatggcac cagagtatgg agcaacttgc
     1261 ggattcttcc cagttgatga tgaatctctt aaatatatga agttaactgg tagatcagac
     1321 gaacatatcg cgctagtaaa agaatatttg aaacaaaacc atatgttctt tgatgttgag
     1381 aaagaagatc ctaattatac agatgttatc gaattggatt tatcaacagt tgaagcatcg
     1441 ctttcaggac caaaacgtcc tcaagattta attttcttaa gtgatatgaa atcatcattt
     1501 gaaaattctg taacagctcc agcaggcaac caaggacacg gtttagataa aagtgaattt
     1561 gataagaaag ctgaaattaa ctttaaagat ggatcaaaag ctacaatgaa aacaggtgat
     1621 attgcaatag cagcaattac atcatgtaca aatacatcta acccttatgt aatgttaggt
     1681 gcaggtttag ttgctaaaaa agcagttgaa aaaggcttga aagttcctga atacgttaaa
     1741 acttctctag caccaggatc aaaagttgtt accggatatt taagagatgc tggcttacaa
     1801 ccttatttag atgatttagg cttcaacttg gttggttatg gatgtacaac ttgtatcggt
     1861 aattcaggtc ctttattacc agaaattgaa aaagcgattg ctgatgagga cctattagtg
     1921 acatctgtat tatctggtaa ccgtaacttt gaaggtcgta tccatcctct tgttaaagcc
     1981 aattacctag cttcaccaca gttagttgtt gcttatgcat tagctggaac ggttgatatt
     2041 gatttacaaa atgaacctat tggtaaaggt aatgacggtg aagatgtata tttgaaagat
     2101 atttggccat caattaaaga agtttcagat accgttgata gtgttgtaac acctgaatta
     2161 tttattgaag aatataataa cgtatacaat aacaacgaat tatggaatga gattgatgta
     2221 actgatcaac ctctatatga ctttgatcct aattcaacat acattcaaaa tccatcattc
     2281 ttccaaggat tatctaaaga accgggtacg attgttccat taaatggttt acgtgttatg
     2341 ggtaaattcg gtgattctgt gacaactgac cacatctctc cagcaggtgc aattggtaaa
     2401 gatacgccag ctggtaaata tttacaagat catcaagtgc ctattcgtga atttaattca
     2461 tatggttcaa gacgtggtaa tcacgaagta atggttcgag gtacgtttgc taatatacgt
     2521 attaaaaacc aattagcgcc aggtactgaa ggtggtttta caacttattg gccaacaaat
     2581 gaagtaatgc ctatctttga tgctgcaatg aaatataaag aagatggtac aggtttagtt
     2641 gtattagctg gtaacgatta tggtatgggt tcatctcgtg actgggcagc aaaaggtaca
     2701 aacttattag gtgttaaaac agttattgca caaagttatg aacgtatcca tcgttcaaat
     2761 ttagttatga tgggtgtatt accattagag tttaaaaaag gtgaatcagc tgattctctt
     2821 ggtctagatg gtacagaaga aatttctgtt aatattgatg aaaatgttca accacatgac
     2881 tacgtcaaag ttactgctaa gaagcaagat ggtgatttgg tagaatttga cgctatggtt
     2941 cgttttgact cacttgttga aatggattac tatcgtcacg gtggaatttt acaaatggtt
     3001 ttaagaaata aattagcgca ataaaaaata gatatcacag taaaatttta atcggtattt
     3061 gaggcacttt gatagtgctc aaaaatactt ctataaactt tatgatataa taggtcggtt
     3121 caaaaccttt gtgttcttgg ccggcctctt cttgtatttt atgttagaat gagatataaa
     3181 gttaaactga aagaaggctt aaaatgatat atagtattac agaaatagaa gcgcgttatg
     3241 ctgaaactga taagatgggt gtaatttatc acgggaatta tgcaacttgg tttgaagttg
     3301 cgcggttgga ttatatatcg aagttaggtt ttagttatgc tgatatggaa aaacaaggaa
     3361 tcatttcacc tgtgactgac ctcaatgtca attataaaaa gtctattttt tatccagaaa
     3421 aagttaaagt taaaacttgg gttgaaaaat attcgagatt acgttcagtg tataaatatg
     3481 agatttttaa tgaaaaaggt gaacttgcaa ctacaggttc cacagaattg atttgcatta
     3541 aagaagatac ttttaagcct atacggttgg atcgttattt cccagattgg catgaagctt
     3601 atagtaaagt gcaaacgctc aataatgaag ggaaaacagt agagataatg gatggtattg
     3661 attctttata agttactgta aatgtttgga gaggcaatat taacaattaa tctataaaaa
     3721 atagccaata cgttaacatt aatgtaatta gcgtgaagat aagctaatat atagcaaaaa
     3781 cgaacttgtt catacaacat cagatgtgct aaaccaaagt acaacttccg aggttcagga
     3841 cacatcagaa tgtgtaaaca agttcgtttt attttagtta ttttgtggaa taagaaattt
     3901 catcttcgtg atcaactaca tttacaataa tgtggtcatc ttcaaagtac cacaaatctt
     3961 tttccgctac gacaacattt aaatcgtcat attgttgttc atagccaata tcaacatctt
     4021 cctttggttc aactgtaaaa gcaggactaa atccttgctt gagttggaat tcgccaccat
     4081 atcttacaaa aaacacgagc actttattat tttcaggcaa ctcaagttca tttttaaacc
     4141 aagttactgc tgcatcagta agttctattt gcataacaat gacctcctat atttattaca
     4201 gaatgggagt gggccaactt gtattatttg tggaatttct tatcgaaatt ctctgtgttg
     4261 gagcccaccc caacctgctc attattgtaa gctgaatttc cataagcttc tatgttggga
     4321 cccccgacca gaattgaaaa aacctagtta taagcgcatt ttgttcggtt aactacagct
     4381 aatataacat tgtggagcct atgacattga tttatgtctc ggtctcaaat gttcttgtaa
     4441 aaaaactaag tatagttgaa ttttaagtta aaaaaattgt ttttgcaaaa ataaaataat
     4501 ccatcaactg caaaaaaata tgcaatgatg gactaatgtc aaaaaattgt tacatccatt
     4561 ttattttagg ttcttcgcct ctaaaaatcc ttgcgatatt agagcgatgt ctaattatca
     4621 atatgattga aactaagaaa ctaacgacta ataaaatata gtcttgaatg ataagcgagc
     4681 caatcacaca gcaaattgct gcaacgatac ttgctaaaga aacatattta aaaatcttca
     4741 atacaataaa gaagataatt gcaagtatta gtaaaagtat cggattgact cccaagacga
     4801 cacctgcact agttgcaaca gctttgccac cttggaattt taaataaaca ggataaacgt
     4861 gtccaagtat agcgaataag ccaacaatta aaccatttgt aaaaaaagta ctaatagggc
     4921 catctgcgtg aacttgtaac cataaaggga agaaaacagt tatgaaccct ttgaaaatat
     4981 ctagaaatgt taccaagaat cctgcaggac gacctaatac tctaaagcta ttagtagcgc
     5041 cagtattacc actaccaaat tgtctaatat cttttttgaa aaataatttt ccaattacga
     5101 atccacttgg gaaagcgccg ataagataac ttagtagtaa catgacgatt atcatcataa
     5161 atattacaca tcctttaata tcttaggact attttatcat attcatttaa attacggcta
     5221 aaaattttaa aaacggggat taatatatgg aattaagcta tgaaagttaa ttgatacttg
     5281 cattttacgc tgatttatat aagaataact attgtatagt tttaaaaacg aacgtacgtt
     5341 tgcaggaggc gaaatcattg gcaatgaata aacaaaataa ttattcagat gattcaatac
     5401 aggttttaga ggggttagaa gcagttcgta aaagacctgg tatgtatatt ggatcaactg
     5461 ataaacgggg attacatcat ctagtatatg aaattgtcga taactccgtc gatgaagtat
     5521 tgaatggtta cggtaacgaa atagatgtaa caattaataa agatggtagt atttctatag
     5581 aagataatgg acgtggtatg ccaacaggta tacataaatc aggtaaaccg acagtcgaag
     5641 ttatctttac tgttttacat gcaggaggta aatttggaca aggcggctat aaaacttcag
     5701 gtggtcttca cggtgttggt gcttcagttg taaatgcatt gagtgaatgg cttgaagttg
     5761 aaatccatcg agatggtaat atatatcatc aaagttttaa aaacggtggt tcgccatctt
     5821 ctggtttagt gaaaaaaggt aaaactaaga aaacaggtac caaagtaaca tttaaacctg
     5881 atgacacaat ttttaaagca tctacatcat ttaattttga tgttttaagt gaacgactac
     5941 aagagtctgc gttcttattg aaaaatttaa aaataacgct taatgattta cgcagtggta
     6001 aagagcgtca agagcattac cattatgaag aaggaatcaa agagtttgtt agttatgtca
     6061 atgaaggaaa agaagttttg catgacgtgg ctacattttc aggtgaagca aatggtatag
     6121 aggtagacgt agctttccaa tataatgatc aatattcaga aagtatttta agttttgtaa
     6181 ataatgtacg tactaaagat ggtggtacac atgaagttgg ttttaaaaca gcaatgacac
     6241 gtgtatttaa tgattatgca cgtcgtatta atgaacttaa aacaaaagat aaaaacttag
     6301 atggtaatga tattcgtgaa ggtttaacag ctgttgtgtc tgttcgtatt ccagaagaat
     6361 tattgcaatt tgaaggacaa acgaaatcta aattgggtac ttctgaagct agaagtgctg
     6421 ttgattcagt tgttgcagac aaattgccat tctatttaga agaaaaagga caattgtcta
     6481 aatcacttgt gaaaaaagcg attaaagcac aacaagcaag ggaagctgca cgtaaagctc
     6541 gtgaagatgc tcgttcaggt aagaaaaaca agcgtaaaga cactttgcta tctggtaaat
     6601 taacacctgc acaaagtaaa aacactgaaa aaaatgaatt gtatttagtc gaaggtgatt
     6661 ctgcgggagg ttcagcaaaa cttggacgag accgcaaatt ccaagcgata ttaccattac
     6721 gtggtaaggt aattaataca gagaaagcac gtctagaaga tatttttaaa aatgaagaaa
     6781 ttaatacaat tatccacaca atcggggcag gcgttggtac tgactttaaa attgaagata
     6841 gtaattataa tcgtgtaatt attatgactg atgctgatac tgatggtgcg catattcaag
     6901 tgctattgtt aacattcttc ttcaaatata tgaaaccgct tgttcaagca ggtcgtgtat
     6961 ttattgcttt acctccactt tataaattgg aaaaaggtaa aggcaaaaca aagcgagttg
     7021 aatacgcttg gacagacgaa gagcttaata aattgcaaaa agaacttggt aaaggcttca
     7081 cgttacaacg ttacaaaggt ttgggtgaaa tgaaccctga acaattatgg gaaacgacga
     7141 tgaacccaga aacacgaact ttaattcgtg tacaagttga agatgaagtg cgttcatcta
     7201 aacgtgtaac aacattaatg ggtgacaaag tacaacctag acgtgaatgg attgaaaagc
     7261 atgttgagtt tggtatgcaa gaggaccaaa gtattttaga taattctgaa gtacaagtgc
     7321 ttgaaaatga tcaatttgat gaggaggaaa tctagtgagt gaaataattc aagatttatc
     7381 acttgaagat gttttaggtg atcgctttgg aagatatagt aaatatatta ttcaagagcg
     7441 tgcattgcca gatgttcgtg atggtttaaa accagtacaa cgtcgtattt tatatgcaat
     7501 gtattcaagt ggtaatacac acgataaaaa tttccgtaaa agtgcgaaaa cagtcggtga
     7561 tgttattggt caatatcatc cacatggaga ctactcagtg tacgaagcaa tggtccgttt
     7621 aagtcaagac tggaagttac gacatgtctt aatagaaatg catggtaata atggtagtat
     7681 cgataatgat ccgccagcgg caatgcgtta cactgaagct aagttaagct tactagctga
     7741 agagttatta cgtgatatta ataaagagac agtttctttc attccaaact atgatgatac
     7801 gacactcgaa ccaatggtat tgccatcaag atttcctaac ttactagtga atggttctac
     7861 aggtatatct gcaggttacg cgacagatat accaccacat aatttagctg aagtgattca
     7921 agcaacactt aaatatattg ataatccgga tattacagtc aatcaattaa tgaaatatat
     7981 taaaggtcct gattttccaa ctggtggtat tattcaaggt attgatggta ttaaaaaagc
     8041 ttatgaatca ggtaaaggta gaattatagt tcgttctaaa gttgaagaag aaactttacg
     8101 caatggacgt aaacagttaa ttattactga aattccatat gaagtgaaca aaagtagctt
     8161 agtaaaacgt atcgatgaat tacgtgctga caaaaaagtc gatggtatcg ttgaagtacg
     8221 tgatgaaact gatagaactg gtttacgaat agcaattgaa ttgaaaaaag atgtgaacag
     8281 tgaatcaatc aaaaattatc tttataaaaa ctctgattta cagatttcat ataatttcaa
     8341 catggtcgct attagtgatg gtcgtccaaa attgatgggt attcgtcaaa ttatagatag
     8401 ttatttgaat caccaaattg aggttgttgc aaatagaacg aagtttgaat tagataatgc
     8461 agaaaaacgt atgcatatcg ttgaaggttt gattaaagcg ttgtcaattt tagataaagt
     8521 aatcgaattg attcgtagct ctaaaaacaa gcgtgacgct aaagaaaacc ttatcgaagt
     8581 atacgagttc acagaagaac aggctgaagc aattgtaatg ttacagttat atcgtttaac
     8641 aaatactgac atagttgcgc ttgaaggtga acataaagaa cttgaagcat taatcaaaca
     8701 attacgtcat attcttgata accatgatgc attattgaat gtcataaaag aagaattgaa
     8761 tgaaattaaa aagaaattca aatctgaacg actgtcttta attgaagcag aaattgaaga
     8821 aattaaaatt gacaaagaag ttatggtgcc tagtgaagaa gttattttaa gtatgacacg
     8881 tcatggatat attaaacgta cttctattcg tagctttaat gctagcggtg ttgaagatat
     8941 tggtttaaaa gatggtgaca gtttacttaa acatcaagaa gtaaatacgc aagataccgt
     9001 actagtattt acaaataaag gtcgttatct atttataccg gttcataaat tagcagatat
     9061 tcgttggaaa gaattgggac aacatgtatc acaaatagtt cctatcgaag aagatgaagt
     9121 ggttattaat gtctttaatg aaaaggactt taatacagat gcattttatg tttttgcgac
     9181 tcaaaatggc atgattaaga aaagtacagt gcctctattt aaaacaacgc gttttaataa
     9241 acctttaatt gctactaaag ttaaagaaaa tgatgatttg attagtgtta tgcgctttga
     9301 aaaagatcaa ttaattaccg tcattactaa taaaggtatg tcattaacgt ataatacaag
     9361 tgaactatca gataccggat taagggcagc tggtgttaaa tcaataaatc ttaaagctga
     9421 agatttcgtt gttatgacag aaggtgtttc tgaaaatgat actatattga tggccacaca
     9481 acgcggctcg ttaaaacgta ttagttttaa aatcttacaa gttgctaaaa gagcacaacg
     9541 tggaataact ttattaaaag aattaaagaa aaatccacat cgtattgtag ctgcacatgt
     9601 agtgacaggt gaacatagtc aatatacatt atattcaaaa tcaaatgaag aacatggttt
     9661 aattaatgat attcataaat ctgaacaata tacaaatggc tcattcattg tagatacaga
     9721 tgattttggt gaagtaatag acatgtatat tagctaaaaa ctatatgcaa tcacgaaatt
     9781 aaatgataaa atacagtaat gttaaatttt gactaaattc aagggattta tattaaatgc
     9841 tgaccaagta cttatcgtta aattagcgat atactttgac caaactaata taaatctctt
     9901 gtattgtata agaaatgatg atgtatctct atatttactg aaggttcaat tcggtctaat
     9961 tttatagaag gtacatgatt catgtagagg atgcgagggg tttattttga aagatttcga
    10021 tagtttaata cctggatggt ttaaagaatt tgtccatgtt ggtaccgatt taatatggtc
    10081 tcaatattta attggtctat tattgacagc tggattcttc tttacgatta gttctaaatt
    10141 cgttcaatta cgaatgttac ctgaaatgtt tagagcttta gtagaacgtc cagaaacttt
    10201 agaagatggt aagaagggta tttcgccatt ccaagcattt gcgattagtg ctggttcgag
    10261 agttggtact ggtaatattg ctggtgttgc gactgcgatt gttttaggcg gtccaggtgc
    10321 agtattttgg atgtgggtta ttgcatttat aggtgcagcg agtgcattta tagaagcgac
    10381 tttggctcag gtttataaag tacatgataa agatggtgga ttccgtggtg gtccagctta
    10441 ctatattact aaaggtttaa atcaaaaatg gctaggtatc gtatttgcga ttttaattac
    10501 aattacattt gcatttgtat ttaacacagt gcaatctaat acaattgcgg agtcgttaaa
    10561 tacgcaatat aatattagtc cagtaatcac aggtattatt ttagcaatcg taacagctat
    10621 tattatattt ggtggtgtac gtagtattgc tacgttatct tcgttaattg taccgattat
    10681 ggctatcatt tacattggta tggttttagt aatattgcta tttaatttag atcaaattgt
    10741 tcctatgata ggtacgatta ttaaaagtgc atttggtatc gaacaagtaa ctggtggcgc
    10801 tgtaggtgct gcggttcttc aaggtatcaa acgtggttta ttctctaacg aagctggtat
    10861 gggttctgcg ccgaatgcag cggcaactgc tgccgtacca caccctgtta agcaaggttt
    10921 aatccaatca ttaggtgtgt tctttgatac aatgttggtt tgtacagcaa ctgcaatcat
    10981 gattttacta tattcaggac tgaaatttgg tgataacgca cctcaaggtg ttgcagttac
    11041 tcaatcagca cttaatgagc atttaggttc tgctggaggt attttcttaa caatagcagt
    11101 tacactgttt gcattttcat ctgttgtagg taattactat tacggtcaat ctaatatgga
    11161 atttttatca acaaaccgtg taatattatt tatctttaga tgtcttgttg tagtacttgt
    11221 ctttgttggt gcagttgtaa aaacagaaac agtatggaat acggcagact tatttatggg
    11281 cttaatggca attgtaaaca ttatttccat tataggactg tccaatgtag cttttgcatt
    11341 gatgaaagat tatcaaaagc agaaaaaaga aggcaagaac cctgtcttta aacctgaaaa
    11401 cttagaaatt aacttatttg gaattagtgc ttggggcgct aacaaatata agaactctga
    11461 taaataaata tttttaatgt aacgattcat tatataaaaa tcaaataaac cctgaggttt
    11521 tcaaattaaa gcgaaaatct cagggtttta aatttgtact caattatcat ctgtatcatt
    11581 acactagtta atctaagatt tgattatata ttaatatagt aaggttgaga tttatttcct
    11641 ccattggtgt gcaaagacta taccattgat aagacatatt gttggataga tgaatataaa
    11701 tttgttttag ctttgcacaa tttaatagta cgttttagtt tg
//
LOCUS       DALAGD010000088        11483 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.089, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000088 DALAGD010000000
VERSION     DALAGD010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11483
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.089"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(48..1304)
                     /locus_tag="O2701_002128"
     CDS             complement(48..1304)
                     /locus_tag="O2701_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3578 domain-containing protein"
                     /protein_id="HCZ4294009.1"
                     /translation="MIDNFMQVLKLIKEKRTNNVVKKSDWDKGDLYKTLVHDKLPKQL
                     KVHIKEDKYSVVGKVATGNYSKVPWISIYDENITKETKDGYYLVYLFHPEGEGIYLSL
                     NQGWSKISDMFPRDKNAAKQRALTLSSELNKYITSNEFNTGRFYYAENKDSSYDLKND
                     YPSGYSHGSIRFKYYDLNEGFTEEDMLEDLKKFLELFNELASKVTKTSYDSLVNSIDE
                     IQEDSEIEEIRTAQKDKTLKEVEAPKGIIPKYKKGVSKTTKNDSEIEKSNKENKLTGK
                     VGEKLALNYFNELIDNKIDEDKKEQFRNILNDNPGSQHGHGYDLVAFDPTNTDKAVEK
                     FIEIKTSTSSSIEEPFFMSLNEMFAMKEYKQKYLILRIFNVSGKEPQFYFIDPYANYS
                     EFKDVDDLIDKVFNVEAIQYKVFGEK"
     gene            complement(1626..2105)
                     /gene="rlmH"
                     /locus_tag="O2701_002129"
     CDS             complement(1626..2105)
                     /gene="rlmH"
                     /locus_tag="O2701_002129"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020363758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCZ4294010.1"
                     /translation="MKITILAVGKLKEKYWKQAIAEYEKRLGPYTKIDIIEVPDEKAP
                     ENMSDKEIEQVKEKEGQRILAKIKPQSTVITLEIQGKMLSSEGLAQELNQRMTQGQSD
                     FVFVIGGSNGLHKDVLQRSNYALSFSKMTFPHQMMRVVLIEQVYRAFKIMRGEAYHK"
     gene            complement(2473..4791)
                     /gene="adsA"
                     /locus_tag="O2701_002130"
     CDS             complement(2473..4791)
                     /gene="adsA"
                     /locus_tag="O2701_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored adenosine synthase AdsA"
                     /protein_id="HCZ4294011.1"
                     /translation="MKALLLKTSVWLVLLFSVMGLWQVSNAAEQHTPMKAHAVTTIDK
                     ATTDKQQVPPTKEAAHHSGKEAATNVSASAQGTADDTNSKVTSNAPSNKPSTVVSTKV
                     NETRDVDTQQASTQKPTHTATFKLSNAKTASLSPRMFAANAPQTTTHKILHTNDIHGR
                     LAEEKGRVIGMAKLKTVKEQEKPDLMLDAGDAFQGLPLSNQSKGEEMAKAMNAVGYDA
                     MAVGNHEFDFGYDQLKKLEGMLDFPMLSTNVYKDGKRAFKPSTIVTKNGIRYGIIGVT
                     TPETKTKTRPEGIKGVEFRDPLQSVTAEMMRIYKDVDTFVVISHLGIDPSTQETWRGD
                     YLVKQLSQNPQLKKRITVIDGHSHTVLQNGQIYNNDALAQTGTALANIGKITFNYRNG
                     EVSNIKPSLINVKDVENVTPNKALAEQINQADQTFRAQTAEVIIPNNTIDFKGERDDV
                     RTRETNLGNAIADAMEAYGVKNFSKKTDFAVTNGGGIRASIAKGKVTRYDLISVLPFG
                     NTIAQIDVKGSDVWTAFEHSLGAPTTQKDGKTVLTANGGLLHISDSIRVYYDINKPSG
                     KRINAIQILNKETGKFENIDLKRVYHVTMNDFTASGGDGYSMFGGPREEGISLDQVLA
                     SYLKTANLAKYDTTEPQRMLLGKPAVSEQPAKGQQGSKGSKSGKDTQPIGDDKVMDPA
                     KKPAPGKVVLLLAHRGTVSSGTEGSGRTIEGATVSSKSGKQLARMSVPKGSAHEKQLP
                     KTGTNQSSSPEAMFVLLAGIGLIATVRRRKAS"
     gene            complement(5019..5819)
                     /locus_tag="O2701_002131"
     CDS             complement(5019..5819)
                     /locus_tag="O2701_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCZ4294012.1"
                     /translation="MSRLIRMSVLASGSTGNATFVENEKGSLLVDVGLTGKKMEELFS
                     QIDRNIQDLNGILVTHEHIDHIKGLGVLARKYQLPIYANEKTWQAIEKKDSRIPMDQK
                     FIFNPYETKSIAGFDVESFNVSHDAIDPQFYIFHNNYKKFTILTDTGYVSDRMKGMIR
                     GSDAFIFESNHDVDMLRMCRYPWKTKQRILGDMGHVSNEDAAHAMTDVITGNTKRIYL
                     SHLSQDNNMKDLARMSVGQVLNEHDIDTEKEVLLCDTDKAIPTPIYTI"
     gene            complement(6209..6997)
                     /locus_tag="O2701_002132"
     CDS             complement(6209..6997)
                     /locus_tag="O2701_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HCZ4294013.1"
                     /translation="MNWKLTKTLFIFVFILVNIVLVSIYVNKVNRSHINEVESNNEVN
                     FQQEEIKVPTSILNKSVKGIKLEQITGRSKDFSSKAKGDSDLTTSDGGKLLNANISQS
                     VKVSDNNLKDLKDYVNKRVFKGAEYQLSEISSDSVKYEQTYDDFPILNNSKAMLNFNI
                     EDNKATSYKQSMMDDIKPTDGADKKHQVIGVRKAIEALYYNRYLKKGDEVINARLGYY
                     SVVNETNVQLLQPNWEIKVKHDGKDKTNTYYVEATNNNPKIINH"
     gene            complement(6998..8332)
                     /locus_tag="O2701_002133"
     CDS             complement(6998..8332)
                     /locus_tag="O2701_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294014.1"
                     /translation="MNNKEHIKSVILALLVLMSVVLTYMVWNFSPDIANVDNTDSKKS
                     ETKPLTTPMTAKMDTTITPFQIIHSKNDHPEGTIATVSNVNKLTKPLKNKEVKSVEHV
                     RRDHNLMIPDLNSDFILFDFTYDLPLSTYLGQVLNMNAKVPNHFNFNRLVIDHDADDN
                     IVLYAISKDRHDYVKLTTTTKNDHFLDALAAVKKDMQPYTDIITNKDTIDRTTHVFAP
                     SKPEKLKTYRMVFNTISVEKMNAILFDDSTIVRSSKSGVTTYNNNTGVANYNDKNEKY
                     HYKNLSEDEASSSKMEETIPGTFDFINGHGGFLNEDFRLFSTNNQSGELTYQRFLNGY
                     PTFNKEGSNQIQVTWGEKGVFDYRRSLLRTDVVLNSEDNKSLPKLESVRSSLANNSDI
                     NFEKVTNIAIGYEMQDNSDHNHIEVQINSELVPRWYVEYDGEWYVYNDGRLE"
     gene            complement(8325..10151)
                     /gene="walK"
                     /locus_tag="O2701_002134"
     CDS             complement(8325..10151)
                     /gene="walK"
                     /locus_tag="O2701_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HCZ4294015.1"
                     /translation="MKWLKQLQSLHTKLVIVYVLLIIIGMQIIGLYFTNNLEKELLDN
                     FKKNITQYAKQLEISIEKVYDEKGSVNAQKDIQNLLSEYANRQEIGEIRFIDKDQIII
                     ATTKQSNRSLINQKANDSSVQKALSLGQSNDHLILKDYGGGKDRVWVYNIPVKVDKKV
                     IGNIYIESKINDVYNQLNNINQIFIVGTAISLLITVILGFFIARTITKPITDMRNQTV
                     EMSRGNYTQRVKIYGNDEIGELALAFNNLSKRVQEAQANTESEKRRLDSVITHMSDGI
                     IATDRRGRIRIVNDMALKMLGMAKEDIIGYYMLSVLSLEDEFKLEEIQENNDSFLLDL
                     NEEEGLIARVNFSTIVQETGFVTGYIAVLHDVTEQQQVERERREFVANVSHELRTPLT
                     SMNSYIEALEEGAWKDEELAPQFLSVTREETERMIRLVNDLLQLSKMDNESDQINKEI
                     IDFNMFINKIINRHEMSAKDTTFIRDIPKKTIFTEFDPDKMTQVFDNVITNAMKYSRG
                     DKRVEFHVKQNPLYNRMTIRIKDNGIGIPINKVDKIFDRFYRVDKARTRKMGGTGLGL
                     AISKEIVEAHNGRIWANSVEGQGTSIFITLPCEVIEDGDWDE"
     gene            complement(10164..10865)
                     /locus_tag="O2701_002135"
     CDS             complement(10164..10865)
                     /locus_tag="O2701_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCZ4294016.1"
                     /translation="MARKVVVVDDEKPIADILEFNLKKEGYDVYCAYDGNDAVDLIYE
                     EEPDIVLLDIMLPGRDGMEVCREVRKKYEMPIIMLTAKDSEIDKVLGLELGADDYVTK
                     PFSTRELIARVKANLRRHYSQPAQDTGNVTNEITIKDIVIYPDAYSIKKRGEDIELTH
                     REFELFHYLSKHMGQVMTREHLLQTVWGYDYFGDVRTVDVTIRRLREKIEDDPSHPEY
                     IVTRRGVGYFLQQHE"
BASE COUNT         3250 a         2232 c         1700 g         4301 t
ORIGIN      
        1 tctattttta ttagatcaca attttatttt agctcttgtt caagtaatca tttttcgcca
       61 aaaactttat actgaatagc ttctacatta aatactttgt caatgagatc atctacatct
      121 ttaaattcag aataatttgc atatggatct ataaaataaa attgtggttc tttaccggaa
      181 acattaaata ttcttaatat taaatatttc tgcttatatt ctttcatagc aaacatttca
      241 tttagcgaca taaaaaatgg ttcctcaata ctagaagatg tagatgtttt aatttcaata
      301 aatttttcta cagctttatc tgtatttgtt ggatcaaaag ctactaaatc atagccatga
      361 ccgtgttgag agcctggatt atcatttaaa atattcctaa actgttcttt cttatcttcg
      421 tctattttat tatcaattag ctcattaaag taatttagcg ctaatttttc tccaacttta
      481 ccggttaatt tattctcttt atttgatttt tcaatttctg aatcattttt agtagtcttt
      541 gatacacctt ttttatattt tggaattatt cctttaggtg cttccacttc cttgagtgtc
      601 ttatcttttt gtgctgttct aatttcttca atttcgctgt cttcctgtat ttcgtctatg
      661 ctattgacca agctatcata ggatgttttt gtaacttttg aagctaattc attaaatagt
      721 tctaaaaatt tctttaaatc ctctagcata tcttcttctg tgaatccttc attcaaatca
      781 taatatttga atcttattga tccatgagaa tatcctgatg gataatcatt ttttaaatca
      841 taagatgaat ctttattttc tgcgtaataa aatcttccag tattaaattc atttgatgta
      901 atatatttat tgagttcgga agataaagtt aatgctcttt gttttgcagc atttttatcc
      961 cgcggaaaca tatcacttat ctttgaccat ccttgattca aagataagta tatgccttct
     1021 ccttccggat gaaaaagata taccaaataa tatccatcct ttgtttcttt tgttatattc
     1081 tcatcatata ttgaaatcca aggaacttta ctatagttcc cagtagcaac cttccctaca
     1141 actgaatatt tatcttcttt tatatgcact tttaactgct tgggtaactt atcatggact
     1201 aaagttttat atagatcacc tttatcccaa tcagattttt taactacatt attggtacgt
     1261 ttctctttaa ttaatttaag gacctgcata aagttgtcta tcatttgaaa ttccctccta
     1321 ttataaaata tattatgtct cattttcttc aatatgtact tatttatatt ttaccgtaat
     1381 ttactatatt tagttgcaga aagaattttc tcaaagctag aactttgctt cactataagt
     1441 attcagtata aagaatattt cgctattatt tacttgaaat gaaagactgc ggaggctaac
     1501 tatgtcaaaa atcatgaacc tcattactta tgataagctt cttaaaaaca taacagcaat
     1561 tcacataaac ctcatatgtt ctgatacatt caaaatccct ttatgaagcg gctgaaaaaa
     1621 ccgcatcatt tatgatatgc ttctcctcgc ataatcttaa atgctctgta cacttgttca
     1681 attaacacaa cccgcatcat ttgatgtggg aatgtcattt tgctgaatga tagtgcgtag
     1741 ttactgcgtt gtaagacgtc cttgtgcagg ccgtttgatc cgccaatgac gaaaacaaag
     1801 tcgctttgcc cttgggtcat gcgttggttc aattcttggg ccaatccttc ggaagatagc
     1861 atctttcctt gtatttctaa tgtaatgact gtggattgtg gtttgatttt ggctagtatt
     1921 cgttggcctt ctttttcttt tacttgctca atttctttgt cactcatatt ttctggtgct
     1981 ttttcgtctg gaacttctat gatgtctatc ttggtgtatg ggcctaaacg tttttcatat
     2041 tctgctatgg cttgcttcca atatttctct tttagtttcc ctacagctaa aatggtgatt
     2101 ttcatgtcgt ttggtcctcc aaattgttat caactttcca gttatccaca agttattaac
     2161 ttgttcacac tgttccctct tattatacca atattttttg cagtttttga tattttcctg
     2221 acatttatgc ccgattttta gtgtataacc tgtgtatttg tggataactc tgtgggcatt
     2281 gtgggtattt ctctttaaat tgtacacaat tctggataac tatccccatt tctgtggata
     2341 aacaccttga tgtcttatta cttatcctcg tttttatatc ccatatttat caacggttat
     2401 cgcttttttt cataaaaaaa cactaccgta cctcttattt aagaaataca gtagtattat
     2461 tttcaatata ttttagctag cttttctacg tcgtacagtc gcgattaaac ctatacctgc
     2521 taataataca aacatcgctt ctgggcttga actttgatta gttccagttt ttggtaactg
     2581 tttctcatgc gcgctacctt taggcactga cattctagcc aattgtttcc cactcttgct
     2641 tgatacagta gctccttcta ttgtgcgacc agaaccttct gtaccgctac taacagttcc
     2701 tctatgcgct agcaacaata caactttacc tggagctggt tttttcgctg gatccatcac
     2761 tttgtcgtca ccaattggtt gtgtatcttt accagactta ctacctttgc taccttgttg
     2821 tcctttagct ggttgttcac ttactgctgg tttacctaat aacatacgtt gtggttctgt
     2881 cgtatcatac ttagctaagt tagctgtttt taaataactt gctagtactt gatctaatga
     2941 aataccttct tctctaggac caccgaacat actatatccg tcgccacctg atgctgtgaa
     3001 gtcattcatc gttacgtgat atacacgttt taaatcaata ttttcaaact tacctgtctc
     3061 tttatttaaa atttgaatag cattaattcg tttgccagac ggtttattta tatcatagta
     3121 aacacggatt gaatcagaga tatgtagtaa accgccattc gctgttaaca ctgtcttacc
     3181 gtccttttgt gttgttggtg cgcctaaact atgttcgaaa gccgtccaga cgtctgaacc
     3241 ttttacatca atttgcgcaa tcgtatttcc aaatggtaat actgagatta aatcatagcg
     3301 tgtcacctta ccttttgcga tagaggcacg aatacctcca ccatttgtca cggcaaagtc
     3361 agtcttttta gagaaattct taacgccata cgcttccata gcatctgcaa tcgcgtttcc
     3421 taaatttgtt tcacgcgttc taacgtcatc tctttctcct ttgaaatcaa tggtattgtt
     3481 tggaataatt acctctgcag tttgtgctct aaatgtttga tcagcttgat taatttgttc
     3541 agctaatgct ttgttcggtg ttacattttc aacgtcttta acattaatca atgacggttt
     3601 aatattcgat acctctccat tgcgataatt aaatgtaatc ttaccgatat tcgcaagtgc
     3661 tgtacctgtt tgtgccaatg catcattgtt ataaatttga ccattttgaa gtactgtatg
     3721 tgaatgacca tcaataactg taatacgttt cttcaattgt ggattttgac ttaattgttt
     3781 cactaagtaa tcaccacgcc atgtttcttg tgttgaagga tcaattccta aatgtgatat
     3841 aacaacaaat gtatctacgt ctttataaat acgcatcatt tccgctgtca cactttgtaa
     3901 tggatctcta aattcaacgc ctttaatgcc ttcaggtctt gttttcgtct ttgtttctgg
     3961 tgtcgttaca ccaataattc cataacgaat accatttttt gttacaatcg ttgaaggctt
     4021 aaacgcgcgt tttccatctt tataaacgtt agtacttagc atcgggaagt ctaacatacc
     4081 ctctaacttt ttcaactgat cgtatccaaa gtcaaattca tggttaccga ctgccatagc
     4141 atcataacct actgcattca ttgctttagc catttcttca cctttagact ggtttgaaag
     4201 tggtaaacct tggaaggcgt ctcctgcgtc taacattaaa tcaggctttt cttgttcttt
     4261 tactgttttt aatttagcca taccgatgac acgccctttt tcttcggcta gtcggccatg
     4321 gatatcattt gtatgtaata ttttatgtgt tgttgtttgt ggtgcattag cagcaaacat
     4381 tcgtggtgaa agtgatgctg ttttagcatt tgataatttg aacgttgctg tgtgagttgg
     4441 tttttgtgtt gaggcttgtt gtgtatctac gtcgcgtgtt tcgtttactt ttgttgaaac
     4501 tactgtagat ggtttgttag atggtgcgtt ggatgttact ttgctgtttg tatcatcagc
     4561 tgttccctgc gctgatgctg atacgttggt tgccgcttct ttgccagaat gatgagccgc
     4621 ttcctttgtt ggcggtactt gttgcttatc tgttgttgct ttgtctatcg ttgttactgc
     4681 atgtgctttc attggtgtat gctgctcagc cgcgttcgag acttgccata atcccattac
     4741 actaaaaagc aaaacgagcc atacacttgt tttaagtaat aaagctttca tttcgttccc
     4801 tccattaact gtcatacacc aatatattat cataatcctt atattaaaat gattgatttt
     4861 atgtaaatta tttataaatt acatttaata tcgaattatt ttacacattt caatcaagcc
     4921 gattcaactc aacaacagcc atttcccacc aacaacataa aaaagcaccc gataaagtcg
     4981 tctcacagca atgcggaact ttatcggatg actctcattt atattgtata tattggcgtt
     5041 ggaatagctt tatccgtatc acatagcaat acttcttttt ccgtatcaat atcgtgttcg
     5101 ttcaatactt ggccaacact catacgcgcc aaatctttca tgttattgtc ttgtgataaa
     5161 tgcgataggt aaatacgttt cgtgttacct gtaatcacgt ctgtcattgc atgagccgca
     5221 tcctcattag atacatgacc catatcgcct aaaatacgtt gtttcgtctt ccatggataa
     5281 cgacacattc tcaacatatc gacgtcatga ttactctcaa aaataaacgc atcgctgcca
     5341 cgtatcatac ctttcatacg atcagacacg taacccgtat ccgttaaaat cgtaaacttc
     5401 ttatagttat tatggaaaat ataaaattgc ggatctattg catcatgtga cacgttaaac
     5461 gattcaacat cgaaacctgc aatagatttt gtttcataag gattaaaaat gaatttctga
     5521 tccataggga tgcgactatc tttcttttca attgcctgcc aagtcttttc attcgcataa
     5581 attggcaatt gatatttacg cgccaaaaca cctaatcctt taatatgatc aatatgttca
     5641 tgggttacta aaataccatt taaatcttga atattacggt caatttgact aaacaattct
     5701 tccattttct ttccagtcaa accaacatca actaatagac tacctttttc attttctaca
     5761 aaagtggcgt tacctgtact accacttgct aatacactca tgcgtatcaa gcggctcatc
     5821 ctttctcttt aaacagtcaa tctctctata tgactgcaat atacatcagt gcaaatatac
     5881 gattttccct acagaaacta tcagtatatt agctgttttg tatacctttc ctattatgta
     5941 actttagttc atcgaccggt ttcttatatt taaattttga taatcacgtg caatgaattt
     6001 cttaattatt ataacaatga ttagacaaat actaaagttt ttaaaataac agttacacta
     6061 attttacaat gcggttctac ttaaaatatg aataaatgcg cacgttggag acgcaaaaac
     6121 atcagccata ataaaccgta ccagcaaaaa agctagcacc gcttctcaca tatgatgagc
     6181 ttgcaatgct agcttattac gattcatatt aatgattaat aattttaggg ttattatttg
     6241 tcgcttcgac atagtaagta ttcgttttat ccttaccgtc atgcttcact ttaatttccc
     6301 agtttggttg taacaattga acattcgttt cattcacgac tgagtagtaa ccgagtctag
     6361 cattaatgac ttcatcacct tttttcaagt aacgattata atataatgcc tcgattgctt
     6421 ttctcacacc aatcacttga tgcttcttat ctgcaccatc tgtgggctta atgtcatcca
     6481 tcattgattg tttataacta gtcgctttgt tatcttctat attaaagttc aacatcgctt
     6541 tactgttatt taaaatcgga aaatcatcat acgtttgttc atattttaca gaatctgaac
     6601 taatctcgct taattgatat tcagcacctt taaatacgcg cttgttaaca taatctttca
     6661 aatcttttaa gttattgtca ctgaccttta ccgattgact aatgttcgca ttcaataatt
     6721 ttccaccatc tgatgtggtc aaatccgaat cgcctttagc tttagaacta aagtcttttg
     6781 atcgccctgt aatttgctct aattttatac ctttaactga tttattcaat atactagtcg
     6841 gtactttaat ttcttcttgc tgaaaattaa cttcattgtt actctcgact tcattaatgt
     6901 gtgagcgatt gactttatta acataaatcg atactaacac gatgttgaca agaataaaca
     6961 cgaaaatgaa aagtgtcttt gtcagtttcc agttcattta ttcaagcctc ccatcgttat
     7021 aaacatacca ttcgccatca tattctacat accaacgcgg tacgagttca ctgttaatct
     7081 gcacttcaat gtgattatga tctgaattat cctgcatttc gtaaccgata gcgatgtttg
     7141 ttactttttc aaaattaata tcactattgt tcgctaagct tgaacgtaca gactctaatt
     7201 tcggcaacga tttattatcc tcactattta aaacaacgtc ggtgcgtaat aacgaacgac
     7261 gatagtcaaa gacgcctttt tcaccccaag tgacttgaat ttgattagaa ccttctttat
     7321 taaacgttgg ataaccatta aggaaacgtt gatatgttaa ctcgcctgac tgattattcg
     7381 tactaaacaa tctaaagtct tcgtttaaga aaccaccatg accattaata aaatcaaagg
     7441 ttcctggaat cgtttcttcc attttgctgg aactcgcttc atcttcggac aggtttttat
     7501 aatgatattt ttcattttta tcgttatagt ttgcgacacc tgtattattg ttgtaggttg
     7561 taacaccact ctttgaacta cgaacgatgg ttgaatcgtc aaatagtata gcattcattt
     7621 tctcaacact aatcgtgtta aataccatgc gatatgtttt taacttttca ggtttacttg
     7681 gtgcaaaaac atgcgtcgta cgatcaattg tatctttgtt tgtgatgata tctgtgtatg
     7741 gttgcatatc ttttttcact gctgctaatg catctaaaaa atgatcattt ttcgttgtag
     7801 ttgttaattt tacgtaatcg tggcgatctt tgcttatagc ataaagcacg atattatcat
     7861 cagcatcatg atctatgacc aaacgattga aattgaaatg atttggtact ttcgcattca
     7921 tgttcagtac ttgaccaaga tatgttgata acggtaaatc atacgtaaaa tcgaataata
     7981 taaaatcact gttcaaatca ggaatcatca agttatgatc acgacgaaca tgttccacgg
     8041 acttcacttc tttatttttc aaaggtttcg tcagtttatt cacattagat accgtcgcaa
     8101 tcgttccttc tggatgatca tttttcgaat gaataatctg aaatggcgta atagttgtat
     8161 ccattttggc tgtcataggt gtcgttaaag gtttcgtttc actcttctta ctatctgtat
     8221 tgtcgacatt tgcaatatca ggagaaaagt tccataccat atatgtcaat acgacactca
     8281 tcaagacgag tagtgctaaa atgacagatt taatatgctc cttattattc atcccaatca
     8341 ccgtcttcaa tgacttcaca tggaagtgtg ataaagatag atgtaccttg accttctaca
     8401 ctgtttgccc aaatacgacc attgtgcgct tccacaatct ctttcgaaat ggctagtcct
     8461 aatccagtac cacccatttt acgcgtacgt gccttatcta cacgatagaa tcggtcgaat
     8521 atcttatcga ctttattgat aggaatacca atgccattat ctttaatacg aatcgtcatt
     8581 cgattataaa gtggattttg tttcacgtgg aactcgacac gtttatcgcc tctagaatat
     8641 ttcatcgcat ttgtaatgac attatcaaat acttgcgtca ttttatcagg atcaaattct
     8701 gtgaaaatcg tctttttcgg aatatctcga ataaatgttg tatctttcgc agacatttca
     8761 tgtcgattaa taattttatt aatgaacatg ttaaagtcga taatttcttt gttgatttga
     8821 tcagactcat tatccatttt agataactgt agcaagtcat tgaccagtcg aatcattcgt
     8881 tctgtttctt cacgggtaac agataaaaat tgtggcgcaa gttcctcatc tttccatgca
     8941 ccttcttcaa gtgcttcaat gtaactattc atagaagtta aaggtgtacg taactcatgt
     9001 gatacattgg caacaaattc acgacgctca cgttcaactt gttgttgttc agttacgtca
     9061 tgtaacacag cgatataacc agttacaaat cctgtttcct gcacaatcgt actaaagtta
     9121 acacgtgcga ttagaccttc ttcttcattt aaatctaata agaaactatc attattctct
     9181 tgaatttctt ccagtttaaa ttcatcttca agacttaata cacttaacat gtaatatccg
     9241 atgatgtctt ctttcgccat accaagcatc ttgagtgcca tatcattgac gatacgaata
     9301 cgtccacggc ggtctgttgc aataatacca tcactcatat gggtgataac tgagtccagt
     9361 ctacgtttct cactttcagt attagcctgc gcttcttgta cacgtttaga caagttatta
     9421 aatgctaaag ctaattcgcc aatttcatca ttaccataaa tcttcacacg ttgcgtatag
     9481 ttacctctgg acatttcgac cgtctggtta cgcatatcgg tgattggttt ggtaatcgtt
     9541 cgcgctataa agaatcctag gatgactgtg attaataatg aaatagctgt accaacaatg
     9601 aatatttgat ttatattatt taattggtta taaacgtcat taatttttga ttcgatataa
     9661 atattaccaa ttaccttttt atcgacttta actgggatat tatataccca gacacggtcc
     9721 ttaccaccgc cataatcttt taaaattaaa tgatcgtttg attgtcctag tgatagtgct
     9781 ttttggacag aactatcatt cgctttttga ttgattagac tacggttaga ctgcttcgtc
     9841 gtcgcaataa taatttggtc tttatctata aaacgaattt ctccaatttc ttgacggttg
     9901 gcatactcac ttaataaatt ttgaatatct ttttgtgcat ttacggagcc cttttcgtca
     9961 tatacttttt caatactaat ttctaattgt ttcgcgtact gcgtaatatt cttcttaaaa
    10021 ttatcaagca gctctttttc aaggttattt gtaaaatata acccgataat ttgcatacca
    10081 atgataatca gtaatacata aacaattaca agtttagtat gaagggattg tagttgtttt
    10141 agccacttca ttcgtttcga cctctactca tgttgttgga ggaaatatcc aacgcctcta
    10201 cgcgtcacaa tatattcagg atgtgacgga tcatcttcaa tcttttcacg taaacgacga
    10261 atcgttacat cgaccgtacg tacatcgcca aagtaatcat agccccatac tgtttgtaat
    10321 aaatgttcac gtgtcattac ttgtcccata tgttttgata aataatggaa caattcaaat
    10381 tcacgatgtg ttaattcaat atcttcgcca cgttttttaa tagaatatgc gtctggataa
    10441 atcacaatat ctttaattgt gatttcattc gttacatttc cagtgtcttg tgctggttgt
    10501 gagtaatgac gacgtaagtt cgctttcaca cgtgcgatta attcacgcgt actaaacggt
    10561 ttcgttacat agtcatctgc acctagttct aaaccaagca ctttatcaat ttctgaatct
    10621 ttagcagtaa gcattattat tggcatttcg tattttttgc gcacttcacg acatacttcc
    10681 ataccatcac gaccaggtaa catgatatct agtaatacga tgtctggttc ttcttcataa
    10741 attaagtcga ctgcatcatt accatcgtat gcacagtaca catcgtatcc ttcttttttt
    10801 aagttaaatt ctaaaatatc agcaatcggt ttttcatcat caactacaac aacttttcta
    10861 gccatttgca taaacctctt ttcttaaatc attaatatta ttttatcatt ttttgttctt
    10921 tatcgtttat gtttgtgtaa aaaatcacag gatgtcgctt actatttttg ttattgtgcg
    10981 cattttccgt cttataacgt atagtaacct attatataat ttaccattta taagctatta
    11041 attctatttt ccaatataaa cttatatatt actatttttt attagtttct cacggcggat
    11101 tgtgacaatg atatttaaca caatgatgag gtctctattc caataataaa attaggataa
    11161 tggcaacttc tagttgcacg tcattgctca gaaaataaat atcattattc gttattcgtt
    11221 actttaacaa ttgatgttat ggaggactgg tggcatatca caacgaagaa atatgttgac
    11281 agtagcatat gtttgtacgt ctaataaaga ttttattgtc tacgtttatc tttcatatcc
    11341 aaccactaag ttacttgagg ttcatacatt ttaaagaaaa tggacgaaca gataaatcaa
    11401 tatgattatc attcgtccat atatctttta ttaatataat attaaacgca aaaaaatgcc
    11461 tatcctcttg gataggcatt tgt
//
LOCUS       DALAGD010000089        11467 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.090, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000089 DALAGD010000000
VERSION     DALAGD010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11467
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.090"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(129..1178)
                     /locus_tag="O2701_002136"
     CDS             complement(129..1178)
                     /locus_tag="O2701_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP synthase"
                     /protein_id="HCZ4294017.1"
                     /translation="MIPTLEEIIDKYGNLVDYLKFDVTVEVYEDLLLLRSLSDLNEHQ
                     KMDRVSFIKKHLNSNFDYLLDDTEFRSINKIYSNLNIMTHINSQNNNFKRNPFINEEQ
                     LKSLLAIKKVDAHMSYDSNITSKALAEINKTQKRLVTKIDTLYNQSKKENEYVRLGEK
                     ISYTKLENHWKYLYNEIQFYNLNNQLISYVALEQEYAWAFLNELFYLIELYLKAFKGQ
                     KKTDYEFGKKLNEFIQSYVIILLIDIRLPVVRLYIIRDIVDTCEGEKDNYKRITLIEE
                     SIKKYRYVKDQIKRFKNGLEESLLNATLSIEQNMLKVKIDYYKAYCFPREKTKHNNIE
                     YNVSLFFKALDALKK"
     gene            1317..1607
                     /locus_tag="O2701_002137"
     CDS             1317..1607
                     /locus_tag="O2701_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294018.1"
                     /translation="MNKKSKQQEKLYNFIIAKSFQQPVGSTFTYGELRKKYNVVCSTN
                     DQREVGRRFAYWIKYTPGLPFKIVGTKNGSLLYQKIGINPCNNSTPSKGGDC"
     gene            1607..3394
                     /locus_tag="O2701_002138"
     CDS             1607..3394
                     /locus_tag="O2701_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HCZ4294019.1"
                     /translation="MSYTLFEIGPFALDSFGWKETIPPKKDGDSEKVVRMASPIIVNA
                     RFSDPITGVEKLIITNNNGKKDIFESDILTTRNLPSLIKYGYSINEKYIRSLSYALQL
                     MRDRLPLSELYEGVGILETPFGYLISLDKVLKSIQFNQSSPSYPIVDSAYDLTPKGTF
                     DNWFNMYIDEVKGHLLLELAVIFGISALVTSFLKHKHEIEFAGILFSFTGQSSTGKST
                     AAALAVSVAGNPTKGNETLFRSWNATRNALEGYLSNNYGIPIVFDELSSTTLRDTTGL
                     LYSIAEGQGRQRSNVHGEVKTPKNWGTSVISTSEYSIFNDSAQNDGLRVRTIEINEQF
                     TTNATNADNIKKAVALNYGHVLPLVAKYLINREDEVIQWFYKEVDWFEAKLKDETNNT
                     GIRMFKRYAVITTSAKILGRVLSTDIDIANIRDYFIDYHTHTVSERSLADKAIDVIIQ
                     FVAQNRGKFSDEGALKNMFENYGLISLKDDHIEVKIIANVFKQMLNNHQFQDVNNVVN
                     ALRDKGFILADRGRQTTKRSVKDNSGKKQSLVFYHLKLDVEFASILGLTKDKSLLQNW
                     TPSNDNKAAKELFKSANEGIGPSGVHEDF"
     gene            3628..4977
                     /locus_tag="O2701_002139"
     CDS             3628..4977
                     /locus_tag="O2701_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCZ4294020.1"
                     /translation="MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQ
                     SGRKDNRSGYRQITQLIQQGQCDILCCYRLNRLHRNLKNALKLIKLCQTYHVHILSVH
                     DGYFDMDQAFDRLKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYEYH
                     NGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYC
                     GRVNNQYGQFDNMFPSIVSTSIYEQAQRLRLQKQTKQTPSDNQLKQKIKCPCCNATLT
                     NMTIRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEVCRDFYQNQRIYTKI
                     KSAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSING
                     HQIQHIIQNIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA"
     gene            4999..6627
                     /locus_tag="O2701_002140"
     CDS             4999..6627
                     /locus_tag="O2701_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCZ4294021.1"
                     /translation="MQQLKTKRVGIYVRVSTEMQSTEGYSIDGQINQIKEYCDFHHFE
                     VKDIYADRGISGKSMNRPELQRMLKDAKEGNIDCVMVYKTNRLARNTSDLLKIVEDLH
                     KQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
                     LPLGYDKIPDSKHELMINQHEANIVKYIFECYAKGHGYRKIANALNHKGYVTKKGKPF
                     SISSITYILANPFYIGKIQFAKYKDWSEKRRKGLNDKPVIAEGKHSPIINQDLWDKVQ
                     MRKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKDGTKKRIRYYSCSNF
                     RNKGSKVCSANSVRADVIEDYVMKQILEIVKSDKVIQRVVTHVNQENQVDGAALHHDI
                     AYKQQQYDEVQIKINNLIKTIEDNPDLTSVIRPSIQKYEKQLNDITNQINQLKNQQNE
                     DKTLFDAKEISKLLQHIFHDIKHIEKSRLKALYLSVIDRIDIKKDGNHKKQFYVTLKL
                     NNEIIKQLFNNKQLDEVHLSTSSLFLPQTLYLTI"
     gene            7149..7499
                     /locus_tag="O2701_002141"
     CDS             7149..7499
                     /locus_tag="O2701_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002502216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294022.1"
                     /translation="MKTITQELKQYITHLFQLSNNEAWECEALEEAAENILPERFIND
                     SPLVHLTLETYTYYNDELHELSIYPFLMYANNQLISVGYLDHFDMDFLYLTDTQNIII
                     DERHLLKQGGQDHE"
     gene            7586..7897
                     /locus_tag="O2701_002142"
     CDS             7586..7897
                     /locus_tag="O2701_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HCZ4294023.1"
                     /translation="MKINRYITRGINESIPLDLQILLWHMVEKKDNQPHTDYLHIFKL
                     QEDENILSITHEQEQPTYKLEYHYTNYEKNQNALPKKVYVIREDDVDAFYYVMLLPEE
                     Y"
     gene            7912..8418
                     /locus_tag="O2701_002143"
     CDS             7912..8418
                     /locus_tag="O2701_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HCZ4294024.1"
                     /translation="MNTIKSTIHTEAIFSDDEQHRYLLKKTWDEKKAACTVITMYPHL
                     DGVLSLDLTTVLILNQLANSERYGAVYLVNLFSNIRTPENLKHIKNPYDEHTDIHLMK
                     AISESDTVILAYGAYAKRPVVIDRVEQVMEMLKPHKKKVKKLINPVTNEIMHPLNPKA
                     RQKWILKS"
     gene            8470..10077
                     /locus_tag="O2701_002144"
                     /pseudo
     CDS             8470..10077
                     /locus_tag="O2701_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011274780.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(10068..10328)
                     /locus_tag="O2701_002145"
     CDS             complement(10068..10328)
                     /locus_tag="O2701_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HCZ4294025.1"
                     /translation="MYVVYRASHETAKEALGDKELRAIAHELTKTVKDNMSVDWSKRD
                     SAKAKMRVQVRRLLKKYGYPPDLQKMAVEQVVEQAELMASQQ"
     gene            complement(10300..11286)
                     /locus_tag="O2701_002146"
     CDS             complement(10300..11286)
                     /locus_tag="O2701_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer MecR1"
                     /protein_id="HCZ4294026.1"
                     /translation="MLSSFLMLSIISSLLTICVIFLVRMLYIKYTQNIMSHKIWLLVL
                     VSTLIPLIPFYKISNFTFSKDMMNRNVSDTTSSVSHMLDGQQSSVTKDLAINVNQFET
                     SNITYMILLIWVFGSLLCLFYMIKAFRQIDVIKSSSLESSYLNERLKVCQSKMQFYKK
                     HITISYSSNIDNPMVFGLVKSQIVLPTVVVETMNDKEIEYIILHELSHVKSHDLIFNQ
                     LYVVFKMIFWFNPALYISKTMMDNDCEKVCDRNVLKILNRHEHIRYGESILKCSILKS
                     QHINNVAAQYLLGFNSNIKERVKYIALYDSMPKPNRNKRIVAYIVCSISSFT"
BASE COUNT         4243 a         1934 c         1744 g         3546 t
ORIGIN      
        1 aacgattcct ctgattattt cacgcgattt ccacaacatt tttcgtattt cagaacgatt
       61 aagactacat tatcctggct taatattaat taattttaat tatatctata aatattttca
      121 gtgaaaaact atttctttaa ggcatctaat gctttaaaaa acaacgatac attgtactca
      181 atattgttat gctttgtttt ttctctagga aagcagtagg ctttataata atcaatttta
      241 actttcaaca tattttgttc aatagataaa gttgcattta ataacgattc ttctaatcca
      301 tttttaaatc ttttaatctg atcttttacg tatctatact ttttaataga ttcttctatc
      361 agtgtaattc ttttgtaatt atctttttct ccttcacatg tatctacaat gtctctaata
      421 atatataatc taacaacagg taatcgtata tctataagta aaataatgac atatgattga
      481 ataaattcgt ttaatttttt cccaaactca taatctgttt tcttttgtcc tttaaatgcc
      541 ttcaaataca attctatcaa ataaaatagt tcatttaaaa aagcccaagc atattcctgt
      601 tctaatgcaa cataactaat caattgatta ttaagattat aaaactgaat ctcattatat
      661 aaatatttcc aatgattctc caattttgta tacgatattt tttctcctaa ccgtacatat
      721 tcattttctt tttttgactg attatagagt gtgtctatct ttgtaactaa tcttttttgc
      781 gttttgttta tttctgccaa tgcttttgaa gtaatatttg aatcataact catatgtgca
      841 tctactttct ttatcgcgag tagtgatttc aattgttctt catttataaa tggattacgt
      901 ttaaaattat tattttgact attgatatgc gtcatgatat ttaaattact gtaaatctta
      961 ttgatacttc taaactcagt atcatctaat aaataatcga aatttgagtt taaatgtttt
     1021 tttataaatg aaactctatc catcttttga tgttcattta aatcgcttaa acttcttaat
     1081 aacaacaaat cttcataaac ctcaactgtt acatcaaatt tcaaataatc aactaagttt
     1141 ccatacttat caataatttc ttctagcgta ggtatcaata tatacccccc ctccccattt
     1201 tttgttccgc gtctaatcat aatgcttcat ttatgcttat tatagcaatc aattgaagat
     1261 agtttaacct atatgatatt gattgactcc ttaaaaatta ttggaggaat tttaaaatga
     1321 ataagaaatc taagcagcaa gaaaagcttt ataattttat aattgctaaa tcttttcaac
     1381 aaccagttgg aagtacgttc acttatggtg aattaagaaa gaaatataat gtggtttgta
     1441 gcacgaatga tcaacgtgaa gtaggacgtc gttttgctta ctggattaag tacacgccag
     1501 gattaccatt taaaattgta ggaacaaaaa atggcagttt attatatcag aaaataggga
     1561 taaacccatg caataatagc acgccttcga aaggaggtga ttgctaatga gttacacatt
     1621 gtttgaaatt ggcccatttg ctttagattc cttcggttgg aaagaaacga taccacctaa
     1681 aaaagatggt gacagtgaaa aagtggttag aatggctagc cctattattg ttaatgcaag
     1741 attttctgat ccaattactg gtgttgaaaa gttgattatt actaacaaca atggtaaaaa
     1801 agacattttt gagtctgata tcttaacaac acgaaactta ccttctttaa ttaaatatgg
     1861 ctacagtatt aacgagaagt atattagatc attgagttat gccttgcaat tgatgcgaga
     1921 tagattacca ctttctgaat tatatgaggg tgtaggtata ctagagacac cttttggcta
     1981 cttgatatcg ctagataaag ttctcaaatc tattcaattt aatcagtcct caccttcata
     2041 ccctattgta gatagtgctt atgacttaac tcctaaaggt acatttgata attggtttaa
     2101 catgtatatt gacgaggtta aaggtcatct tcttttagaa ttggcagtca tatttgggat
     2161 ttctgcctta gtgacaagtt ttctgaagca taaacatgaa atcgaatttg caggaattct
     2221 ctttagtttt acaggtcaat cctcaaccgg taagtcaacc gcagctgctt tagctgtatc
     2281 agtcgctggt aaccctacca aaggtaatga aactcttttt agaagttgga atgccacacg
     2341 aaacgcactt gaaggttatt tgagtaacaa ttatggcatt cctatagtat ttgatgagct
     2401 ttcatctacg acacttagag ataccacggg tttgttatat tccatcgctg aaggtcaagg
     2461 tcgtcaacgt tcaaacgttc atggagaagt taaaacgcct aagaattggg gtacttctgt
     2521 catttcaacg tcagaataca gtatttttaa tgattcagct caaaacgatg gattacgtgt
     2581 aagaaccatc gagattaatg aacaattcac tacaaatgcg accaatgctg acaatattaa
     2641 aaaagctgta gcactaaact atggtcatgt tttaccgtta gtagctaaat atcttataaa
     2701 tcgtgaagat gaagttattc aatggtttta caaggaagta gattggtttg aagctaaatt
     2761 gaaagatgaa acaaacaata caggtattcg tatgtttaag cgttatgcag tcattacaac
     2821 atctgcgaaa atattaggtc gtgttttatc tacagatatt gatatcgcta atataagaga
     2881 ctactttatt gattatcata ctcatacagt ctctgaacgc tcattagctg ataaagctat
     2941 tgatgtcatc attcaatttg tggcacaaaa cagaggtaag ttttcagatg aaggtgcttt
     3001 gaaaaatatg tttgagaact atggattaat ctcattaaaa gatgaccata tcgaagtaaa
     3061 aataattgcg aacgttttta aacagatgct aaacaatcat caatttcaag atgttaataa
     3121 tgttgtcaac gccttaagag ataaaggttt catattagct gaccgtggac gccaaacaac
     3181 taaaagaagt gtcaaagata atagcggtaa aaaacaatca cttgtatttt accatctaaa
     3241 attagatgta gagtttgcat ctatcctcgg attaactaaa gataagtcgt tacttcaaaa
     3301 ttggacaccg tcaaatgata acaaagctgc gaaagaactt tttaaatccg ctaatgaagg
     3361 gataggccct tcaggagttc acgaagattt ctaattgtct ttatcataca actgtgaagc
     3421 atcaaactga ataatcgacg tattgaagat agaacaaata acggagtgaa aaacatggat
     3481 gaacttatcg agatattagc cgatttggta attgaagaaa tcaatcaaac aaatgactaa
     3541 tatggacgcc caacatctca ttgatgttgg gcttatatat caattcatcc ataaatatat
     3601 attatcactg tgaaaggagg agccgatatg aaacaagtca taggctattt acgtcaaagt
     3661 acgatgaaac aacaatctct tgcagcacag aaacaagcta tcgaagcaat agccgaaaaa
     3721 catcatattc aacatatcaa cttttatagc gacaaacaat caggtcgcaa agataatcgt
     3781 agtgggtatc gacaaataac acaattaatt caacaaggac agtgtgacat attatgctgt
     3841 tatcgtctta ataggttgca tcgtaatctg aaaaatgcat taaaactcat caaattatgt
     3901 caaacgtacc atgttcatat cttaagcgta catgatggtt actttgatat ggatcaagct
     3961 ttcgaccgac tcaagcttaa tatcttcatc agtttagccg aacttgaatc ggataacatt
     4021 ggagaacaag tcagaaatgg gcttcaagaa aaagcaaagc aaggtcgatt gattacaaca
     4081 catgcacctt ttggttacga atatcacaac ggaacattca tcatcaatca aaatgagtca
     4141 ccaacggtaa aggctgtatt caattattac attaaaggtc atggttataa gaaaattgca
     4201 cagttattag aagaagataa cacgtatatc aatcgacaac cctatcaagt tcgtaacatt
     4261 atcatcaatc ctaattattg tggtcgtgtc aacaatcaat atggccaatt cgacaatatg
     4321 tttccttcta ttgtttccac aagtatatat gagcaagcgc agagacttcg attgcaaaaa
     4381 caaaccaaac agacaccttc ggataatcaa ctcaaacaaa aaatcaaatg cccatgttgt
     4441 aatgcaacac ttacaaatat gaccattaga aaaaagaatc atacattacg ttactacgtc
     4501 tgtcctaaaa acatgaatgc ttcacgcttt gtctgtgatt ttaaaggcat caatgcacaa
     4561 acacttgaag ataaagtatt agaagtgtgc cgagactttt atcaaaatca acgcatctac
     4621 acaaaaatta aaagtgcgat tgacaaacgc atcaaaagac aaagaaacat agaaaaacat
     4681 cacacattga ctcaagaaca actgatagaa aagttggcac aaggcatcat tgatgcagaa
     4741 acgttcagag agcaaacaca atcattacgt caacaaccgc aacgcactac atctatcaat
     4801 gggcatcaaa tacaacacat cattcaaaat attattcaaa aacgtttcac gttaaacata
     4861 ttgtacccct atattgaaac cattcacatt acgaaagata aaaatcttat aggaatctat
     4921 ttcaaaaatg aaccactcaa tattgtcaat caaaccatgc aatcatcgat tgcataaatg
     4981 aagaaaggat gtaacataat gcaacaactt aaaacaaaac gtgtcggtat ctatgtacgt
     5041 gtatcaacag aaatgcaaag cacagaaggt tatagtatcg acggacaaat caatcaaatc
     5101 aaagaatact gtgacttcca tcattttgaa gttaaagata tatacgctga ccgtggtatt
     5161 tcaggtaaat ctatgaacag acctgagctc caacgtatgt tgaaagatgc aaaagaaggc
     5221 aatatcgact gtgttatggt atacaaaaca aaccgattag ctcgtaatac atctgatctt
     5281 ctcaaaattg tcgaagattt acacaaacaa aatgtcgaat ttttcagttt gtcagagcgt
     5341 atggaagtca atacttcttc tggtaaactc atgttacaga tacttgcgag tttctcagaa
     5401 ttcgaacgta ataacattgt cgagaacgta tttatgggtc aaacgagacg tgcccaagaa
     5461 ggctattatc aaggcaattt accactaggt tatgacaaaa taccagatag taaacacgag
     5521 ctaatgatta accaacatga agctaatatt gtaaaatata tattcgagtg ctatgccaaa
     5581 ggacatggct atcgtaaaat tgccaatgca ttgaatcaca aaggatatgt cactaaaaag
     5641 gggaaacctt tcagtattag ttcaatcaca tacatcttag ctaacccttt ctatatcggc
     5701 aaaattcaat ttgcgaaata caaagattgg agtgaaaaac gtcgtaaagg gctgaatgat
     5761 aaaccagtga tagctgaagg taagcattcc cccattatta atcaagattt atgggataaa
     5821 gtacaaatgc gtaagaaaca agtcagtcaa aaaccccaag tccatggcaa aggaacgaat
     5881 ctgcttacag gcattattca ctgtccccaa tgtggcgcac ctatggcagc aagcaatacc
     5941 acgaatactc ttaaagacgg gactaagaaa cgtattcgtt actattcatg tagtaatttt
     6001 cggaacaagg gttccaaagt atgttcggca aacagtgtaa gagctgatgt gattgaagat
     6061 tatgtgatga agcaaatact tgaaatagtc aaaagtgata aagtcattca acgcgtagta
     6121 acacacgtca atcaagaaaa tcaagttgat ggcgctgcac ttcatcacga tattgcttat
     6181 aagcaacaac aatatgatga agtacaaatc aaaataaata acttgattaa aaccatcgag
     6241 gataatccgg acttaacatc agtaatcaga ccaagtattc aaaaatatga aaagcaactc
     6301 aatgacatta cgaatcaaat caaccaactc aaaaatcaac aaaatgaaga taagacttta
     6361 tttgatgcca aagaaatcag taaactatta caacacatct ttcatgatat taagcacata
     6421 gaaaaatctc gactcaaagc attgtatcta tcagtgattg atcgcattga tattaaaaaa
     6481 gatggtaatc ataaaaaaca attctatgtc acactcaaac ttaataacga aatcattaaa
     6541 caacttttca ataataaaca actcgacgaa gtgcatctca gcacttcgtc tttatttttg
     6601 ccccaaacac tatatcttac tatttagcta atagaatcat tgttataaca ataattttca
     6661 ctatattctt tcgcttattt tatctaacta ttttcaagaa ttgctcttta tcaatttcaa
     6721 taacacttat ctaatgaaac agttattgtc gtaatttttt cataactata caatgcttat
     6781 attgaaattg ctaaagaaca tcccacaatg attttttatc gggaaatgat tgggatttgc
     6841 agattcgaag aaacagaagg attgtgcaag cacaatccca aaatcccgtt actttttata
     6901 aataaataat atatataagg aagcagagac acaaccctat catcaatact tataaataat
     6961 gagttgattg tttgtcaaaa taggcggaat ttcaggattt ccttggcata ccaacgtttg
     7021 tagcgggttt ttatatggaa ttttctctta ttcccaccgg gattcgtaat aaatgacaaa
     7081 tcaaatatat attatctcaa tgatgtatat gaactacatc actattacac tatctaggag
     7141 gcaataatat gaaaaccatc actcaagaac tcaaacaata tataacccac ctattccaac
     7201 tatctaataa cgaagcttgg gaatgtgaag cgctggagga agcagcagaa aatattctac
     7261 cggaacgttt tattaatgat tctccactgg tacatctcac actcgagact tatacctact
     7321 ataatgatga actacatgag ctaagtatct atccatttct aatgtatgcc aataaccaac
     7381 tcatcagtgt cggatactta gaccattttg acatggactt tctgtacctt acagatactc
     7441 aaaatatcat tattgatgaa cgtcatttac taaaacaagg aggtcaggac catgaataat
     7501 tggatcatag ttgctcaatt atccgtcaca gttatcaacg aaatcatcga catcatgaaa
     7561 gaaaagcaga agggagggaa atagcatgaa aatcaatcga tacatcacaa gaggaattaa
     7621 tgaaagtatt ccactagacc ttcaaatctt actttggcac atggtagaaa aaaaagataa
     7681 ccagcctcat acagattacc tacacatttt taaattacaa gaagatgaga atatactctc
     7741 aatcacacat gaacaagaac aacccacata caaattggaa tatcactata caaactatga
     7801 aaaaaatcaa aatgcattac ctaagaaagt ctacgtcatt cgtgaagatg acgtggacgc
     7861 tttttattat gtgatgcttt taccagaaga atactaaagg aagtgacgat gatgaataca
     7921 atcaaaagta cgatacacac agaagccata tttagcgatg atgaacagca ccgctattta
     7981 ctcaagaaaa catgggatga aaagaaagct gcatgcacag tgataacgat gtatcctcat
     8041 ttagacggtg tattatcact cgatctcaca actgttctta tcctcaacca attagccaat
     8101 tcagaacgat acggtgctgt atatcttgta aatctattct ctaatattag aacacccgaa
     8161 aacctcaaac atatcaaaaa tccatacgat gagcacactg atattcattt gatgaaagcg
     8221 attagtgaaa gtgacacagt gattcttgct tatggtgcct atgcgaagcg accagttgtt
     8281 atcgaccgtg tcgaacaagt gatggaaatg ttaaaacctc ataaaaagaa agtaaaaaag
     8341 ctcatcaatc cagtaacaaa tgaaattatg catccactca accctaaggc acgtcaaaaa
     8401 tggattttga aatcatagca aatacatgga ggtcaatatg gaaacaaaac aaatcaacat
     8461 cgttttactg taaatgatat tgttgaaaca attcctgaca atgaattcga cgaattcaga
     8521 catcaccgtg gtgcaacttc gtaccatcct aaaatgatgt taaaagtgat tctatatgcc
     8581 tacacacaat ctgtattctc aggtcgtaaa atagaaaaaa tgcttaatga tagcatccga
     8641 atgatgtggc tatcacaaaa tcaaaaacct tcttataaaa caattaatca atttagagta
     8701 aatccaaaag tagatgcttt attagaatct ttatttattc aattttacag tcagtgtgta
     8761 aaacaaaatc ttatagatga taaagctatt tttattgatg gtacaaaaat tgaagcaaat
     8821 gccaatcgat atacatttgt atggaaaaag agtattcaaa accatgaatc aaagatgaat
     8881 gaggattcta aagccctcta ccatgaattg gtaaccaata aaatcatacc ggaaattaaa
     8941 gaagatcatg ataatgaatt aacaaaagaa gaaatagatt tgattggtag tcacttagat
     9001 aaagaaatcg aagatttaaa ccaacatatc aacaatgaaa aatgtactaa aacaagaaaa
     9061 caaatacgtc tcaaaagaac taaaatcaaa aaatacaaaa agcaaatcaa tgattatttt
     9121 gagcgaaagt atcgatacga atttcaaaaa tctattttaa aggatagaaa tagttattct
     9181 aagacagatt atgatgcgac atttatgaga atgaaagaag atcacatgaa aaatggacaa
     9241 cttaagccag ggtataattt acaaatagca acaaattccc aatttgtttt atcttataat
     9301 gtgtatcaaa atccaacgga tactagaacg atgattccat ttttaaattc aattcaagag
     9361 acctacggtc atttacctga atatattgta gctgatgcag gttatggtag tgaatcaaat
     9421 tataaggcaa ttatagatga ctttaatcga acgccactca taacatatgg aatgtttata
     9481 aaagataaaa ctaaaaaata taaaagtgac atctttaata ctcaaaattg gaactatgac
     9541 gaaattaatg acgaattcat ttgtccgaat aataaacggc taggttttaa aagatatgcc
     9601 tatcgtcatg ataagtatgg ttataagcga gacttcaaat tatatgaatg tgatgattgt
     9661 tcagaatgtc ctctgaaaaa tcaatgtatg aacttcaatt caaaaacaaa caaaaaaata
     9721 atgaagaatt ataactggga atattttaaa tcccaaatta acaaaaagct ttcagaacca
     9781 gaaacaaaaa atatctacag tcaaagaaaa attgatgtgg aacctgtttt tggatttatg
     9841 aaggctattt tgggtttcac tcggatgtct gtccgaggac tcaataaagt caaaagagaa
     9901 cttggttttg tattaatggc acttaatata agaaaagtag tagctcaacg agctgaaaat
     9961 aatcaaaaaa tttataaaaa agacaatttc tatattattt caatagaaat tgtctttttt
    10021 cacttatcca agaactttat gtcccggact cattatgatt tattttttta ttgctgactt
    10081 gccattaatt ctgcttgctc tacaacttgt tccacagcca ttttttgaag atctggtgga
    10141 tagccatatt tctttaatag gcgtctaact tgaactctca ttttagcttt agcactgtct
    10201 cgtttagacc aatcaacact catgttatcc ttaactgttt tagttaactc atgtgcaatg
    10261 gctcttaact ctttatcgcc caaagcttct ttagctgttt catgtgaagc tcgatatact
    10321 acatacaata tacgcaacaa tacgcttgtt tcgattaggt ttaggcattg aatcataaag
    10381 tgcaatatac ttaacacgtt ctttaatatt tgaattaaaa cctagtaaat attgtgctgc
    10441 cacattattt atgtgctgag attttaaaat agagcatttt aatatcgatt caccataacg
    10501 tatatgttca tggcgattca aaatttttaa aacgtttcta tcacatactt tttcacagtc
    10561 attgtccatc attgttttac ttatatatag tgcaggatta aaccagaata tcattttaaa
    10621 aacaacataa agctggttga atattaagtc atgacttttc acatgtgata gttcatgtag
    10681 aataatatat tcaatttctt tgtcattcat ggtttcgact acgacagttg gtagtacaat
    10741 ttgggatttc actaaaccaa ataccatcgg attatcaatg tttgaactat aactaattgt
    10801 tatatgcttt ttgtagaact gcatcttact ttgacatact ttaagtcgtt cattaagata
    10861 tgacgattcc aatgacgaac ttttaataac atcaatttgt cggaatgcct taatcatata
    10921 aaataagcac aacaaactac caaataccca tatcaaaaga atcatatacg ttatatttga
    10981 ggtctcaaac tgattaacat taattgctaa gtctttcgta acagatgatt gttgaccatc
    11041 taacatatga ctaaccgaag aagtcgtgtc agatacattt cgattcatca tatcttttga
    11101 aaatgtaaaa ttcgatattt tgtaaaatgg tattaatgga attaacgtgg agacgagcac
    11161 taataaccaa atcttatgtg acataatatt ttgagtatat tttatataga gcattctcac
    11221 taaaaaaatt acacatatcg tgagcaatga actgattata cttaacatta aaaaagatga
    11281 taacaccttc tacacctcca tatcacaaaa attataacat tattttgaca taaatactac
    11341 atttgtaata tactacaaat gtagtcttat ataaggagta tattgatgaa aaagataaaa
    11401 attgttccac ttattttaat agttgtagtt gtcgggtttg gtatatattt ttatgcttca
    11461 aaagata
//
LOCUS       DALAGD010000090        11443 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.091, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000090 DALAGD010000000
VERSION     DALAGD010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11443
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.091"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..591
                     /locus_tag="O2701_002147"
     CDS             <1..591
                     /locus_tag="O2701_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCZ4294027.1"
                     /translation="KDKQTIIQYIVNYDVNITEKKEVKVKKKKKSEKDKDEYETKTEE
                     KQRKVNQNILINIPIKSENNKYVVVEYPYFTPIPDSQLNQAKMVEDNLKDNKREDNPK
                     AKAFIEDFFNKYASSKSDDMAYLMDNPEGLEGTREVSQIREIRLYPKGDDYVAKVEIL
                     MKDKDSPLENLEHYTLDITKKDGKYYVKNMTNSIGG"
     gene            596..856
                     /locus_tag="O2701_002148"
     CDS             596..856
                     /locus_tag="O2701_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294028.1"
                     /translation="MSGLFNFFVLGADRPSLGGVGDWISNEVGTGIGLVVLVVGIVKW
                     ASGKYGHMVILFVVGGFLFLVSKGPEQVFNAIAGVWKMIFGG"
     gene            868..1251
                     /locus_tag="O2701_002149"
     CDS             868..1251
                     /locus_tag="O2701_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCZ4294029.1"
                     /translation="MDKKAYNLKRTFEQPIVMYEFTDKFRINKGFRLDFWATFLIVWF
                     ILFLLFWYLLQPVIMSIGGLMFIYFTVAPYYITKYIVKLKQDGKKLFFFLWDFVIFVF
                     NVQLRKVKLSYDEEVEYHDKKITFK"
     gene            1286..3781
                     /locus_tag="O2701_002150"
     CDS             1286..3781
                     /locus_tag="O2701_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCZ4294030.1"
                     /translation="MNLKAPYAGLRDNLLLTKTGEVWAYYRVRSESISTANYDAKEES
                     KKRMRYFLESIKGYQDFHFEMYDHDLDLRNKFKEISKDFDDEAFNVAEYYSKETIDVL
                     EQELQMITHNSFVMGVKIRELADDAVTLKEILKSTLSDTAERIVSNFGFDVNLDDKIL
                     DKYKTFERDLADSFLGIGGERLTENELAYIIRKPFISNATHDVQEESSRRAITDIYNA
                     VIDPTRLSVLEVENDNEKFYTTTVVVDDFPLDMEYTHLFEKAQNMPFPVECHVKAKII
                     NNEKVKKKFDRAKINHKQQAKEKQDTGDSASEDVQDNLFVLNQMEREVTGSGVFIEWV
                     FMFVIKSDDYRDCKRKAKRLMKRLKETQIYAVNPLADQLQLFYQCLHGNDLFINKYWL
                     QRTTATGIAENLFGVSQSLGTKTGFYIGRIDKFIRSVSREEAVASSRDIILFSLLLAA
                     KGIKGAVSDSPHVLITGQTGKGKSFLAKLLWIYTSFFKGQMLYWDPKSEFAEWFDRVT
                     ESKEMQEKYPLFINHLKTFKYVTLNYEDSTNYGCLDPIVFLDGIEANEMLKSVFDEIK
                     SFENYHHIETAIYQAISDTVEERENGKKVGSLNVIEKLQNNEDEHVKNAGDLLFEKTQ
                     NNILKLVFSDGTNPALNIQEKATILQVKGLDMPKADDDTSSYSTSEKNGITLMLLIGK
                     FLEKFGSRRDVQTTIFIDEGWAFSASRQGKKVGKRIKRTGRSENNSLVFITQSVKDKA
                     DDDGGNFGCHFAFDEKDEREDILKSLGLEYSKESPENMEMLKDLKKGQCIFSDFYGRV
                     GKMVVHCPFEEMTEAFRTQEDSASSKAEEKFAM"
     gene            3835..5193
                     /locus_tag="O2701_002151"
     CDS             3835..5193
                     /locus_tag="O2701_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HCZ4294031.1"
                     /translation="MAMNEIALFKGVRIQPYQKYIDFMFASVLAFIFLVYRIYILFID
                     YKSVNKKLDILTLFAYFKPHLLYLLIGTAIIFVICKLIAQFLIRFKTKDLAEMIETQG
                     FHNRTVIKKTTEYIPIDYKQTEYDYYPTMFYKRGKKSFVIHIKKDGSRFQDNYLELET
                     IIEPMFNSELVEKKHIGRYLRYEFMPLKYKKRIVMNGEQESNTIFYDTKIAITHQITW
                     DFVKAPHGLVTGITGGGKTYFLFYVIRELFRRHSEVRLLDPKVSDLSFMKRVIGDDKV
                     ADTKGQILKQLREANNEMEERFRLMNDSSDYKIGNDFRNFDMRPYFIIFDEVTAFTST
                     LDKKELQEMNDYLINIIMKGRQAGVFMFLTAQRPDADVIKGNVRDQLGLRVSLGNLSN
                     DGYRMTFGQTDKEFQTIHDSDIGRGYISILGQYNEPILFDAPLMEQYDFVEDVKQILN
                     KE"
     gene            5198..7045
                     /locus_tag="O2701_002152"
     CDS             5198..7045
                     /locus_tag="O2701_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294032.1"
                     /translation="MKHKSVYLLCFLILPILLLTSVSAYAVSNPVGEPSHAVKPKIEK
                     YDLSHYRSIYSEKGDWNPFGQEEISRQINNVSDFFFSMTKILAGVTDYAIENLFQLDV
                     INDFADKIGDFVGDIYQKLLSNLALTLFIIVCFNAFIIFSVQGNAREALKRAFLIMCL
                     IGFGVGILANAGNIIRGTNNIGKDLNNIIMNSTSSIDGNIDYIHENSGMNHIRNQLFD
                     MTIYRTYLVMNYGTVDEKEIKAKGKDRIDNILKLDYDKKSEKELDDIVKDEVEKKNNK
                     YMTQGYVFQKLAISVIGFIITLFMSIVFLSISFAKLIFSTFALFLFLFLVFSWIVSFI
                     PGFELSVFSAFAKTLGYIILSACMTFLFVIVGLCIKLANSFIEPDSQNAYFLNSIFII
                     VILFVMYKKRAQIINFVSRGNISFSPSAIGAGVMNRTQERFNKIRHEQAQNKKAKREN
                     QRDEPAPPLQNDNDLRRRQQDKPMPLFINKDNQKNGNKRREQQESMNGNDVKSASVES
                     NANNYSKQPQKASQQEHQVRETRQRKDIQRSPQVVNQPLNNENHSINRKEQKSVQTAY
                     DTDVQKRQIQNATQNQQSRQSGNRNQPITRNSQSKDRLKEQKDINKHGK"
     gene            7035..8081
                     /locus_tag="O2701_002153"
     CDS             7035..8081
                     /locus_tag="O2701_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCZ4294033.1"
                     /translation="MVNKKDIAKKVIGKTPIGVKLKLAKVIIILCVVAFFIFPVIIMF
                     LLAPDLNKGKDDAGCTVSGGNVSANGIDKFNENAKGGKLEGKGKEIQKIAEKNKVPVN
                     IFMAIIASESQWGKGENATRQNNPLSVMGSKSIHDSTYPTIEDGLNAGAKNLYDVYIS
                     KGLDTPKKIGPKYAPVGASNDPNNMNARWIPTVEKIMKDLGGSEAKTSCSNGKGKSIK
                     FNGKLPHWSNDDPGKGNLYTAGQCTWYAYGMRQKMGKPVSTYWHDAHKWNDRAKAEGY
                     KVDKNPEPGALFIAEQGAGGHDGHYGHVAVVIGVSDGGKTFRITEMNWEGAFKVNERT
                     LKMTDGYSFIHDKE"
     gene            8088..8678
                     /locus_tag="O2701_002154"
     CDS             8088..8678
                     /locus_tag="O2701_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294034.1"
                     /translation="MKQKIIMFTLVTVILFCAVLIGYQIPKQQVKMKQNQIEDLQEEQ
                     RILRDKNGELNKLVKRQSKTVISDEEKQIREVSSNFVKQMFEMKKDSSFKSKAPQIKP
                     LVTKDYYDTLFKDSKDKYDLYDDITVNDIHVYFDTYDPKKDSYKVFVQFDERIETDGD
                     DKIEHRQTSAQLDLVRTAEGWRIDNLKRFNLKPLGR"
     gene            8734..9090
                     /locus_tag="O2701_002155"
     CDS             8734..9090
                     /locus_tag="O2701_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystatin-like fold lipoprotein"
                     /protein_id="HCZ4294035.1"
                     /translation="MRRWFVLILGLVILLSACGQKYDKEIDEVTKLEKESIQDVKNTK
                     KYKNVERSKSYYKIYNDGEVIIMTYMPFKDSNTKVSRVYKINQTSDKYEEDSNIDAEK
                     FEKDNKPVYEENNMKK"
     gene            9199..9702
                     /locus_tag="O2701_002156"
     CDS             9199..9702
                     /locus_tag="O2701_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCZ4294036.1"
                     /translation="MKKRKKRKELDYKTRLAIAKYLNDGLNIIQISKILGRDDAVLYR
                     EVNKRSVDGVYNPLLAEEDAIKSRSISDEQVAKRRRLNDETKAYIEEKLSLKWSPRQI
                     ASQIEKDTGYYISYPTIYRYIRNGLVKVDVKRDMRQAGKKYNKSSEKRGKLDVGSRVI
                     KYRLKKY"
     gene            9683..10219
                     /locus_tag="O2701_002157"
     CDS             9683..10219
                     /locus_tag="O2701_002157"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCZ4294037.1"
                     /translation="MGSKSINNREEFGHYEIDTVWSVRPSTYCLLTIIERKTRYLYAS
                     RLNTRKSNVVCNEIINIMKPLLPKSITMDRGKEFALFEEIEDELNCSIYFSDLGCPYQ
                     RGSIENANGLLRQYYPKGTDFANITQKSLDEAVKQINARPRMIFDYKSSEEMLKYHVS
                     TQNCEPILNDCVRHEPVN"
     gene            10439..10987
                     /locus_tag="O2701_002158"
                     /pseudo
     CDS             10439..10987
                     /locus_tag="O2701_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
BASE COUNT         4158 a         1276 c         2136 g         3873 t
ORIGIN      
        1 aaagataaac aaacaattat acagtatatt gttaattatg atgtgaatat tactgaaaag
       61 aaagaagtta aagttaagaa gaaaaagaaa agtgaaaaag ataaagatga gtatgaaaca
      121 aaaacagaag aaaagcaacg taaagtaaat caaaacattt taataaatat acctattaag
      181 agtgaaaata ataagtatgt tgttgttgaa tatccttatt ttacgccaat ccctgatagt
      241 caattgaatc aagctaaaat ggttgaggat aatttgaaag acaataaacg ggaagacaat
      301 ccgaaagcaa aagcttttat tgaagatttc tttaataaat atgcttctag taagtctgat
      361 gatatggcct atttaatgga taaccctgag ggcttagaag gaactagaga agtttctcag
      421 ataagagaaa ttaggttata tccaaaaggc gatgattatg ttgctaaagt tgaaattttg
      481 atgaaagata aagattctcc tttggagaat ttggagcatt acacattaga tataacaaaa
      541 aaagatggta aatattacgt taaaaatatg actaattcta ttggaggtta atgttatgag
      601 tggtttattt aatttttttg ttttaggtgc tgaccgtcca tcacttggtg gagttggtga
      661 ctggataagt aatgaagtag gtacgggtat tggtttagta gtattggttg ttggtattgt
      721 aaaatgggct agtggtaaat atgggcatat ggttatatta tttgttgttg gtggcttttt
      781 attcttagtt tctaaaggtc ctgaacaggt atttaatgcg attgcaggtg tttggaaaat
      841 gatttttggc ggttgaggtg attccatttg gataaaaaag cttataattt aaagcgaact
      901 tttgaacaac cgatagttat gtatgaattt acagataagt ttcgtattaa taagggtttt
      961 cgtttagatt tttgggctac atttttgatt gtgtggttta tattattttt gttattttgg
     1021 tatttattac agcctgtaat tatgtcgatt ggtggactta tgtttattta ctttacggtt
     1081 gcaccttatt atataacgaa atatattgtt aagttaaaac aagatggaaa gaaattattt
     1141 ttcttcttat gggattttgt aatatttgtt tttaatgtgc agttaagaaa agttaaatta
     1201 agttatgatg aagaagtaga atatcatgat aaaaaaataa catttaaata gcccctattg
     1261 gggttatttt tattggaggg atgacatgaa tttaaaagca ccgtatgcag gtttaagaga
     1321 taatttgtta ttaacgaaaa ctggcgaagt ttgggcgtat tatcgtgttc gttctgagtc
     1381 tatatcaaca gcaaattatg atgcaaaaga agaatcaaaa aagcgtatgc gttatttttt
     1441 agagtcgatt aaaggttatc aagattttca ttttgaaatg tatgaccacg atttagattt
     1501 gcgaaataaa tttaaagaaa ttagtaaaga ttttgatgat gaagctttta atgtagctga
     1561 atattattca aaagaaacta ttgacgtatt agaacaagaa ttacaaatga ttacgcataa
     1621 ttcttttgtt atgggtgtta aaatacgtga attagctgac gatgcagtta cacttaaaga
     1681 gattttaaaa agtacattat cagatacggc tgagcgtatt gtttcgaatt ttggttttga
     1741 tgtgaattta gacgataaaa ttttagataa gtataaaaca tttgaacgag atttggctga
     1801 tagttttcta ggtattggag gcgagcgttt aactgagaat gaattggctt atattattag
     1861 aaagcctttt atttcaaatg caacacatga cgtacaggaa gaatcttcaa gaagagctat
     1921 tacagatatt tataatgcag taattgaccc tactcgcttg agtgttttag aagttgaaaa
     1981 tgataacgag aaattttata caacaacagt tgttgttgat gattttccgt tagatatgga
     2041 atatacacat ttatttgaga aagctcaaaa tatgcctttt ccggttgaat gccatgtaaa
     2101 agcaaaaatc attaataatg agaaagttaa gaaaaaattt gaccgtgcga aaataaatca
     2161 taaacaacaa gcaaaagaaa agcaagatac tggcgacagt gcttcggaag atgttcaaga
     2221 taatttgttt gtattgaatc aaatggaacg agaagtgact ggctcaggtg tatttattga
     2281 gtgggtattt atgtttgtaa ttaaaagtga tgattataga gattgtaaac gtaaagcaaa
     2341 gcgtttgatg aaacgattaa aggaaactca aatatatgca gttaatccat tagctgacca
     2401 gttacaattg ttttatcaat gtttacatgg taatgattta tttattaata agtattggtt
     2461 acaaagaaca acggcaacag gtatagcaga gaatttattt ggtgtgtctc aaagtttggg
     2521 aacaaaaacg ggtttttata tagggcgtat tgataaattt atacgttctg tttcacgtga
     2581 ggaagctgtt gcaagttcaa gggatataat tttgtttagt ttattgttgg ccgcaaaagg
     2641 tattaagggg gctgtcagtg atagtccgca tgttttaatt actggtcaaa ctgggaaagg
     2701 taaatcattt ttagcaaaat tattatggat ttatacatca ttttttaaag gtcaaatgtt
     2761 gtactgggat ccaaaatctg agtttgcaga atggtttgac agagtaacag aaagtaagga
     2821 aatgcaagaa aagtatcccc tatttattaa tcatttaaaa acatttaaat atgtaacatt
     2881 gaattatgaa gattcaacca attatggttg tctagaccct attgtatttt tagatggtat
     2941 tgaagctaat gagatgttga aatcagtgtt tgatgaaatt aaatcttttg aaaattatca
     3001 tcatattgaa acagcaattt atcaagctat ttctgatacg gttgaagaac gtgaaaatgg
     3061 taaaaaagtt ggttcattaa atgtaattga aaaattacag aataatgaag atgagcatgt
     3121 aaaaaatgca ggcgatttat tatttgaaaa aacgcaaaac aatattttaa aacttgtttt
     3181 tagtgatggt acgaatccag ctttaaatat tcaagaaaaa gcaactattt tacaggttaa
     3241 aggtttagat atgcctaaag cagatgatga tacatcaagt tattcaacaa gtgagaaaaa
     3301 tgggattaca ttaatgttat taattggtaa gtttttagag aaattcggtt ctcgtagaga
     3361 tgttcaaacg actatattta ttgatgaggg ttgggctttt agtgcttcaa gacaaggtaa
     3421 aaaagttggt aagcgtataa aaagaacggg acgttctgag aataattcac ttgtatttat
     3481 tacacaatcg gtaaaagata aagcagatga tgatggtggt aactttggtt gtcattttgc
     3541 atttgatgaa aaagatgaac gtgaggatat tttaaaatct ttaggcttag aatattcaaa
     3601 agaatctcct gaaaacatgg agatgttgaa agatttgaag aaaggtcaat gtatattcag
     3661 tgatttttat ggacgtgttg gaaaaatggt tgtacattgt ccatttgagg aaatgactga
     3721 agcatttaga acacaagaag attctgcaag ctctaaagct gaagaaaaat ttgcgatgta
     3781 atttttagca catacaaaat tgtatgtgct ttttatttta aaggagtgga tttgatggca
     3841 atgaatgaaa ttgctttatt taaaggcgta cgtatacagc cttatcagaa gtatattgat
     3901 tttatgtttg caagtgtatt agcatttatt tttttggtat atcgtatata tattttgttt
     3961 attgattata aatcagttaa taaaaaattg gatatattga cgttgtttgc atattttaaa
     4021 ccgcatttat tgtatttatt aattggtaca gcaattatat ttgtgatatg taagcttatt
     4081 gctcagtttt taatacgttt taaaacaaaa gatttggctg aaatgattga aacacaaggt
     4141 tttcataatc gtacagtaat caagaaaaca actgagtata tccctattga ttataaacaa
     4201 actgaatatg attattatcc aacaatgttt tataagcgtg gtaaaaaatc ttttgttatt
     4261 catattaaga aagacggttc gaggtttcaa gataattatt tagagttaga gacgattatt
     4321 gaaccaatgt ttaattctga attagtagaa aaaaagcata ttggtcgtta tttgagatat
     4381 gaatttatgc cacttaaata taaaaagcgt attgttatga atggcgaaca agaatcaaat
     4441 acaatttttt atgatacaaa aattgctatt acgcatcaaa ttacttggga ttttgtgaaa
     4501 gcacctcatg gattggttac aggtataacc ggaggtggta aaacatattt tttattttat
     4561 gtgattcgtg agttattccg tagacattca gaagtacgtt tattagaccc aaaagtttca
     4621 gatttgagct ttatgaagcg cgttattggt gatgataaag ttgcagatac taaaggtcaa
     4681 atattaaaac aattgagaga agctaataat gaaatggaag agcgttttag attaatgaat
     4741 gatagttcgg attataaaat cgggaatgat ttccgtaatt ttgatatgcg cccctatttc
     4801 attatttttg atgaggtaac agcttttact tcaactttag ataaaaaaga attacaagaa
     4861 atgaatgatt atttaattaa tattattatg aaaggtcgtc aagctggtgt atttatgttt
     4921 ttaacagcac aaagaccaga tgcagatgtg attaaaggta atgtgcgtga ccaattaggg
     4981 ttacgtgtgt ctttaggtaa tttgtcaaat gatggttatc gtatgacctt tggtcaaact
     5041 gataaagaat ttcaaacgat tcatgatagt gatatcggcc gaggttatat ttctatactt
     5101 ggacaatata atgaaccaat tttatttgat gcacctttaa tggaacaata tgattttgtg
     5161 gaagatgtga aacaaatatt aaataaggag tgatttgatg aagcataaaa gtgtgtatct
     5221 tttatgcttt ttaattttgc ctattctgct tttaacatct gtgagtgctt atgctgtttc
     5281 taatccagtt ggagagccct cacatgcagt taagccaaaa attgaaaagt atgatttatc
     5341 acattatcgt tcaatatata gtgaaaaagg cgactggaat ccgtttggtc aagaagaaat
     5401 ttcacgtcaa attaataatg tgtcagattt tttcttttct atgactaaga ttttagcagg
     5461 tgttacagat tatgcgattg aaaatttatt tcaattagat gttattaatg attttgcgga
     5521 taaaatcggc gattttgttg gcgatatata tcaaaagtta ttgagtaatt tagcattaac
     5581 attatttatt attgtttgtt ttaacgcatt tattattttt agtgtacaag gtaatgcaag
     5641 agaagcatta aaacgagcgt ttttaattat gtgtttaatt ggttttggtg ttggtatact
     5701 tgcgaatgca ggtaatatta tacgtggtac aaacaatatt ggtaaggact taaataatat
     5761 aattatgaat agtacatctt ctattgatgg taatattgat tatattcatg agaattcagg
     5821 tatgaatcat atacgtaatc aattatttga tatgacgata tatagaacat atttagtaat
     5881 gaattatgga actgtcgacg aaaaagagat aaaagcaaaa ggtaaagacc gtattgataa
     5941 cattttaaaa cttgattatg ataaaaaatc agaaaaagaa ttagatgata ttgttaaaga
     6001 tgaggttgaa aagaaaaata ataaatatat gacgcaaggt tatgtatttc aaaagttggc
     6061 tatttcagtg attggtttta ttattacact ttttatgtcg atagtatttt tatcaataag
     6121 ttttgcgaag ttgatttttt ctacatttgc tttattttta ttcttatttt tagtattcag
     6181 ttggatagtg agttttatac caggatttga attatctgtt ttctctgcgt ttgcaaaaac
     6241 gttaggctat attattttaa gtgcttgtat gacattctta tttgtgatag ttggtttgtg
     6301 tatcaagctt gcaaatagtt ttattgaacc ggatagtcaa aatgcttatt ttttaaattc
     6361 aatatttata attgtaatat tgtttgtaat gtataaaaaa cgggctcaaa ttatcaattt
     6421 tgtaagtcgt ggtaatatta gtttttctcc tagtgcaatt ggtgctggtg tgatgaatcg
     6481 aacacaagaa cgttttaata agattcgtca tgaacaagcg caaaataaga aagctaaacg
     6541 agaaaatcaa agagatgaac ccgccccacc acttcaaaat gataatgatt taagaagaag
     6601 acaacaggat aaaccaatgc ctttatttat aaataaagat aatcaaaaaa atggtaataa
     6661 gcgcagagaa caacaagagt caatgaatgg taacgatgta aaatctgctt ctgttgaatc
     6721 aaatgcaaat aattattcta aacaaccaca aaaagcatca cagcaagaac atcaagtacg
     6781 agaaacaaga cagcgaaaag atatacaaag gtcgccacaa gtggttaatc agccattaaa
     6841 taatgagaat cattcaataa atcgtaaaga acagaagtca gttcaaactg cttatgatac
     6901 tgatgttcaa aaacgacaaa tacaaaatgc gacacagaat caacaaagta gacaatctgg
     6961 aaatcgtaat caacctatta cgcgtaattc tcagtcaaaa gaccgtttaa aagagcaaaa
     7021 ggatatcaat aagcatggta aataaaaaag atattgctaa aaaagtaata ggtaaaacgc
     7081 caataggcgt taaattgaaa ttagcgaaag tcatcatcat tttatgtgtg gtggcttttt
     7141 ttatatttcc agtaattata atgtttttgt tagcgcctga tttaaataaa ggtaaagatg
     7201 atgctggttg tactgttagt ggcggtaatg tttcggcaaa tggtattgat aagtttaacg
     7261 aaaatgctaa aggtggaaaa ttagagggta aaggaaaaga aatacagaaa attgcagaaa
     7321 aaaataaagt acctgtgaat atttttatgg ccattattgc gagtgaatca caatggggta
     7381 aaggagaaaa tgcgacaaga caaaataatc ctttgtctgt tatgggttca aaatcgattc
     7441 atgattcaac atatccaact attgaagatg gtcttaatgc gggagctaaa aatttgtatg
     7501 atgtttatat ttctaaaggt ttagatactc ctaaaaaaat aggtcccaaa tatgcgcctg
     7561 ttggtgcgtc aaatgaccct aataatatga atgcccgttg gataccaaca gttgaaaaaa
     7621 ttatgaaaga tttaggcggt tcagaagcta aaacgtcatg tagtaatggt aaaggaaagt
     7681 ctataaagtt taatggtaaa ttgccacatt ggtcaaatga tgaccctgga aaaggtaatt
     7741 tatacactgc tggtcaatgt acttggtatg catacggtat gcgtcaaaaa atgggtaaac
     7801 ctgtatcgac ttattggcat gatgcacata aatggaatga tagagctaaa gctgagggtt
     7861 ataaagttga taaaaatccg gaacctggtg cattatttat tgctgaacaa ggtgcaggcg
     7921 gtcatgatgg gcattatggt catgtggctg ttgttattgg tgtaagtgat ggtggtaaaa
     7981 catttagaat aacagaaatg aattgggaag gtgcttttaa agttaatgaa cgtacattga
     8041 aaatgactga cggatatagt tttattcatg ataaggagtg aaaataaatg aagcaaaaaa
     8101 ttattatgtt tactcttgta acagtaatat tattttgtgc tgttttaatt ggttatcaga
     8161 ttcctaaaca acaagttaaa atgaaacaaa atcaaattga agatttgcag gaagaacaac
     8221 gtatattaag agataaaaat ggagagttaa ataaattagt aaagagacaa tcaaaaacag
     8281 ttatttctga tgaagaaaaa caaatacgtg aagtttcttc aaattttgtt aaacagatgt
     8341 ttgaaatgaa aaaagattct tcttttaaat caaaagcacc acaaataaag cctttagtta
     8401 caaaggatta ttacgataca ttatttaagg attctaaaga taaatatgat ttatatgatg
     8461 atataacggt taatgatatt catgtttatt ttgatactta tgaccctaaa aaagatagtt
     8521 ataaagtttt tgttcagttc gacgaacgta ttgaaacaga tggagatgat aaaattgaac
     8581 atcgtcaaac atctgcacaa ttggatttag ttcgtacagc tgaaggttgg cgtattgata
     8641 atttaaaacg tttcaattta aaacctctag gacgttagtt tgtttcaaga cgatagcaat
     8701 gatataattt acgtaaaaag gagtgaattt atcatgaggc gatggtttgt attaatttta
     8761 ggattagtta tattattatc tgcatgtggt cagaaatatg ataaagaaat tgatgaagtt
     8821 acaaagttag aaaaagaaag tattcaagat gtgaaaaata caaagaagta taaaaatgtt
     8881 gagcggtcaa aaagttatta taagatttat aatgatggtg aggttattat aatgacttat
     8941 atgcctttta aagatagtaa tacaaaagtt tcaagagtat ataaaataaa tcagactagc
     9001 gataaatatg aagaagattc taatattgat gctgaaaagt ttgagaaaga taataaacca
     9061 gtttatgaag aaaataatat gaaaaaataa agaatcaact ccctatacgc tttagcgatg
     9121 gtatcgggag ttgatgaaca tatatcctct tataacttat tttataaata acctttttaa
     9181 aagtaaagga ggatatacat gaaaaaaaga aaaaaacgta aagaattaga ctataaaaca
     9241 cgtctagcta tcgcaaagta tttaaatgat ggtttaaaca tcattcagat ttctaagatt
     9301 ttaggtcgtg atgatgcagt tctttataga gaagtaaata agcgttctgt tgatggtgtt
     9361 tataatcctt tattagctga ggaagatgct ataaaatcgc gtagtatttc agatgaacaa
     9421 gttgctaaac gtagacgttt gaatgatgaa acaaaagcat atattgaaga aaaactttca
     9481 ttaaaatggt ctcctagaca gatagcaagt caaattgaaa aagatacagg gtattatata
     9541 agttatccta ctatatatag atatattcgc aatggattgg taaaagttga tgttaagcgt
     9601 gatatgagac aagcaggcaa gaaatataat aagtcttctg aaaagcgtgg caaattagac
     9661 gtagggtctc gtgttataaa gtatcggctc aaaaagtatt aataatcgtg aagaatttgg
     9721 acattatgaa attgatactg tttggagtgt tagaccatca acatattgct tgttaactat
     9781 tattgaaaga aagacaagat atttatatgc tagtagatta aatacacgta aatccaatgt
     9841 agtttgtaat gagattataa atattatgaa accattatta cccaaaagta taactatgga
     9901 tagaggtaag gagtttgctc tatttgaaga aatagaagat gaattgaatt gtagtattta
     9961 ttttagtgat ttgggttgtc catatcaacg tggttctatt gaaaatgcaa atggactttt
    10021 aagacagtat tatcctaaag gtacggattt tgcaaatata acacaaaagt cattagatga
    10081 agctgttaaa caaattaatg cacggcctcg aatgatattt gactataaat cgagtgagga
    10141 aatgctaaag taccatgtaa gcacacaaaa ttgtgaacct atattaaatg actgtgtgag
    10201 gcacgaaccc gtcaattaat ataggttaat ctaagcgaat ggtttacaat gagcgcggaa
    10261 ggacaggacg aataaatttt gtatgtaaaa tgcataatct aaaaagaagc atactattta
    10321 aatctgtggt ttttgacaga tattactagt aaaatgcttc tttttcttct tctgtactct
    10381 tttagtaata tttattaaaa aaatttaaga aattctcatt tttctattga cacactgcca
    10441 tttaattaaa gcaattggag tattgttact cattgtcggc atcgtcataa tgaatcaatt
    10501 taacaagaat aatttattac taactgatca aaagtattta ctgttttggc ttctattagg
    10561 atttattttt ggtttctttc cacctattca aacgacaatt aatagtgctt tagctagtca
    10621 tactcattca ccagcctttg catcattagt atcatttaca attgggtcaa tagcgctatt
    10681 gattttaacc gctattttta atcgttcttt aaaactaaaa acaagtcatt taaaattcgg
    10741 taaattaaag cctatctatt ttactggcgg tatacttggc atggcttttg taacagctaa
    10801 cattatctta atgcctcata tgggtgcagc attaacaaca cttattggga tgtttggcca
    10861 gattctaatg ggcatattga tagatcactt tggattattt ggttcaccta aaatagcaat
    10921 gacatccaga aaaactattg gtctattatg tattttgaca ggcattatac ttttaagatt
    10981 attttaaatt aacttttagc ttatcatttt aacttgtaat tatttttaaa agtgataagc
    11041 tatttttttg tggtctaaaa atctttagaa aagcgttgat ttgggtagta tgctttgaca
    11101 caacaaattt taatttagca aattcgatag tcaactcatt cttaagacct aaattaatgt
    11161 tattttttaa taatttacac caaattaata gcaaaaatta tgttattcgt gctaatattt
    11221 catagttggt tattcaatta attaaaaata agtcaaaatg cacaactttt tataattcat
    11281 tgagtcgagt ttgaaaaata aaagtgcttt aatgcatgat caattatcgt actttctatt
    11341 atttgttacc cgttatcaat cggaataacg tatagacact ttaacgtgct atagattggt
    11401 tttaatcact aaattaatgt gtttttctta tcattaaaac tgc
//
LOCUS       DALAGD010000091        11395 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.092, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000091 DALAGD010000000
VERSION     DALAGD010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11395
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.092"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            160..798
                     /locus_tag="O2701_002159"
     CDS             160..798
                     /locus_tag="O2701_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HCZ4294038.1"
                     /translation="MDDLNKKYLIDLHQHQNSSIEVLREFAEVNEVPIVDRLTLDLIK
                     QLIRMNNVKNILEIGTAIGYSSMQFASISDDIHVTTIERNETMIQYAKQNLATYHFEN
                     QVRIIEGNALEQFENVNDKVYDMIFIDAAKAQSKKFFEIYTPLLKHQGLVITDNVLYH
                     GFVSDIGIVRSRNVRQMVKKVQDYNEWLIKQPGYTTNFLNIDDGLAISIKGE"
     gene            801..1724
                     /locus_tag="O2701_002160"
     CDS             801..1724
                     /locus_tag="O2701_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCZ4294039.1"
                     /translation="MTELLVTPKSLSHMETLIDLGADAFVIGEQKFGLRLPGEFNRQQ
                     MTEAVALAHKNDKKVYAAVNGLFHNYHLDAVEDYINFLHEIRVDRIIFGDPAVVMYVK
                     AQENPIPLHWNAETLVTNHFQCNYWGKRGASRAVLARELNLDEIINIKENSNVEIEVQ
                     VQGMTCMFQSKRMLLGNYYTFQDRQMKIERRNDEQSLLLYDEERQNNYPVYEDYNGTH
                     IMSPNDICLIEELAPFFEAGIDSFKIDGILQTEEYINVVTEQYRQAIDLYNEDPEIYE
                     DEKFMLMDPIEEIQPDHRPFDEGFLYKQTVY"
     gene            1736..3004
                     /locus_tag="O2701_002161"
     CDS             1736..3004
                     /locus_tag="O2701_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCZ4294040.1"
                     /translation="MKTIEEIKSTPKTVMKKPELLAPAGNLEKLKIAVHYGADAVFLG
                     GQEYGLRSNADNFTMEEIAEGVEFANRYGAKIYVTTNIIAHDENIEGLESYLRNLEKT
                     GATGIIVADPLIIETCKEVAPKLEIHLSTQQSLSNYKAVEYWKEEGLDRVVLARETGA
                     MEMREMKEKVDIEIEAFIHGAMCIAYSGRCTLSNHMTARDSNRGGCCQSCRWDYELLE
                     VDDNGELDVFYNQGEVTPFAMSPKDLKLIESIPQMMDIGVDSLKIEGRMKSIHYIATV
                     VSVYRKVIDAYAADPDNFKINPEWLIELDKCANRDTAPAFFEGTPGYEEQMFGQQQSK
                     KSPFDFCGLVLDYNEDTKIATIQQRNNFKPGQEIEFFGPEIETFTQVVEAIYDEEGNS
                     LDAARHPLQIVQIKVDRPIYPNNMMRKEIG"
     gene            3004..3627
                     /gene="udk"
                     /locus_tag="O2701_002162"
     CDS             3004..3627
                     /gene="udk"
                     /locus_tag="O2701_002162"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCZ4294041.1"
                     /translation="MKATTIIGIAGGSGSGKTTVTNEIMKNLEGHSVALLAQDYYYKD
                     QKHLTFDERLETNYDHPFAFDNDLLIENLKDLKNGKAVEVPTYDYASHTRSDITIDFK
                     PKDVIIVEGIFALENKVLRDMMDVKIYVDTDADLRILRRLTRDTKERGRSMDSVINQY
                     LSVVRPMHDQFIEPTKKYADIIIPEGGSNKVAIDIMTTKIQSLVSKQ"
     gene            3655..4131
                     /gene="greA"
                     /locus_tag="O2701_002163"
     CDS             3655..4131
                     /gene="greA"
                     /locus_tag="O2701_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCZ4294042.1"
                     /translation="MENQKQYPMTQEGFEKLERELEELKTVKRPEVVEKIKVARSFGD
                     LSENSEYDAAKDEQGFIEQDIQRIEHMLRNALIIEDTGDNNVVKIGKTVTFVELPGDE
                     EESYQIVGSAESDAFNGKISNESPMAKALIGKGLDDEVRVPLPNGGEMNVKIVNIQ"
     gene            4457..5191
                     /gene="pxpB"
                     /locus_tag="O2701_002164"
     CDS             4457..5191
                     /gene="pxpB"
                     /locus_tag="O2701_002164"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCZ4294043.1"
                     /translation="MDVRFINEQTIMIYFENKISEETYRNVTAMVRWIREKEILEIQD
                     IVPSYRAVLIYFDEQAITSSKLIENLELNKFNEKNVHAVNQTNRIIKIPVQYGGTYGP
                     DIEEVAKHNRITVEQVIEKHTSKPYLIYMLGFMPGFPYLGGLDEQLHTPRRNQPRLKI
                     HAGSVGIANNQTGLYPSDSPGGWQIIGRTPLKVFSSEREPMSMYEAGEWIQFYAIDEQ
                     KFIQIERDISDGNFNVDDWVVIENVN"
     gene            5181..6191
                     /locus_tag="O2701_002165"
     CDS             5181..6191
                     /locus_tag="O2701_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HCZ4294044.1"
                     /translation="MSIKILQPGLFSTVQDLGRIGYEHIGFSGAGAMDQFSFKVAQSL
                     INNDGPAIEYTLIGPTIQFNTQNTFVITGGSINASLNNKTISMNSVILAEKGDILKIG
                     AITKGARGYLTFGHSINVPSIAESYATHTRSSIGGFKGRKLLADDVITVKINNDFKEN
                     IGKTIHLQDDLLPENNIIHILQGPQFEAFSEEARAKIVNHPYLITEQSDRMGYRLEGD
                     SVAPINQADIISEPVALGSVQVPNDGQPIILLNDKQTIGGYTKIATVCKFDLPKLAQM
                     KPQDTIQFKWISFQEAVDKNREQMSLFNEILKSHQKTPIFDTSSLRHTSKKLATILKG
                     DL"
     gene            6193..6642
                     /locus_tag="O2701_002166"
     CDS             6193..6642
                     /locus_tag="O2701_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HCZ4294045.1"
                     /translation="MNIEKIEQIIKLVKENDVKKFKYKNFEDEIEIDFTDSNHLAAHS
                     NQSNQSMNNNDLTASKANDNSDVSTNDYHDIKSPMIGTFFLQDSKELTEPIVNVGDKV
                     NKGDIIGYVEAMKVLNEVTTDVAGEITEIVADHGTNVEYDQVLVRIK"
     gene            6656..8017
                     /locus_tag="O2701_002167"
     CDS             6656..8017
                     /locus_tag="O2701_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCZ4294046.1"
                     /translation="MLRCLIANRGEIAVRIIRACREYGIETVAVYAKGDEQSLHVHLA
                     DQAICIGEANALDSYLNIDRIISAAQITGANAIHPGYGFLSESTKFAQTVEEQGIAFI
                     GPTKKTMEMMGDKITARQTVHHAGVPVIPGSNGAVNHVSEIENLAKDIGYPVVIKAAS
                     GGGGKGIRIVKKAEDLEKAFKEAKSEGKKYFDDDRVYVEAFIPVAKHVEVQVMGDGQD
                     NYVHLGERDCSVQRKNQKLIEESPCAALTEERRQQICNDAVKVARAANYRSAGTIEFL
                     VTDTAHYFIEMNARIQVEHTVTEMRAERDLVAAQLYLLEHNHLPFSQSDIQFNGHVIE
                     ARINAENPEKKFQPTPGKVTALHLPQGFNVRVDSLLYHGYQVSPYYDSLVAKVIVKSH
                     DRASAIDKLKVTLDEMVIDGFSTTADFLYAVLNYPLYRDGDAKDVDIKFLEKHQIVKG
                     VEL"
     gene            8017..8769
                     /gene="pxpA"
                     /locus_tag="O2701_002168"
     CDS             8017..8769
                     /gene="pxpA"
                     /locus_tag="O2701_002168"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCZ4294047.1"
                     /translation="MRVDLNCDLGEAFGNYSFGGDHQIIPLITSANVACGFHAGDENV
                     MNETVKLAKAHNVAVGAHPGLPDLKGFGRRNIDISNDEIYNLMIYQLGALQGFCRIHQ
                     VKINHVKPHGALYQMGAKDREIANVIAQAVYDFDPSLVLVGLANSYLISEAKNVGLIT
                     ASEVFADRRYEDDGQLVSRKESDAVITDTDEALKQVLKMVKENKVISKNNKEVTLQAD
                     TICVHGDGEHALLFVSKIREILMKEGIDIQSL"
     gene            8782..10011
                     /locus_tag="O2701_002169"
     CDS             8782..10011
                     /locus_tag="O2701_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HCZ4294048.1"
                     /translation="MGRNLKLKKESDFEFTKNHKRLLLGSVFLMATSAIGPAFLTQTA
                     VFTSQFFASFAFAILLSIIIDIGAQINIWRILVVTGLRGQEISNKVVPGLGTVISILI
                     AFGGLAFNIGNIAGAGLGLNAIFGLDVKWGAAITAIFAILIFVSKSGQKIMDVVSMIL
                     GIVMILVVAYVMFVSNPPYGDAFVHTFAPEHPMKLVLPIITLVGGTVGGYITFAGAHR
                     ILDSGIKGKQYLPFVNQSAIAGILTTGIMRTLLFLAVLGVVVTGVTLSSENPPASVFE
                     HAIGPIGKNIFGIVLFAAAMSSVIGSAYTSATFLKTLHKSLNERSNLIVIVFIVISTM
                     IFLFIGKPISLLIIAGAINGWILPITLGAILIASKKKSIVGDYKHPNWMFIFGIVAVL
                     VTILTGIFSFKEVLQLF"
     gene            10300..>11395
                     /locus_tag="O2701_002170"
     CDS             10300..>11395
                     /locus_tag="O2701_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HCZ4294049.1"
                     /translation="MGYRTLKSIFHEHNESKMKEEYIKRFNSLASFNTNINIIPMENG
                     KKVNDLEYPLFFMVTKNLSKKQELISINSRKIDRALNSLPYAAREQYFNDLLIDELQS
                     TNEIENVFSTKQEIAHALNNQASEFLKFRGLVDQYKEIELNKKIKVDNVRDIRAIYDK
                     LVSNEINEQDKLDGELFRKNFVGVHDGSTNKYIHVGLQPETKIVEFIGEMLTFLKYFD
                     APQPFKIMASHYLFEYIHPFYDGNGRVGRFIIAKLLSDYYDNYTALTFSYVINRNKSK
                     YYKAFMTASNHLNCGDLTEFIDTMLELLIAGQERILDELIPKMDATEKLTLYLTSHYK
                     QIDYEFLYLLSMDKLFGNKRNRLTLIDLENI"
BASE COUNT         4123 a         1531 c         2205 g         3536 t
ORIGIN      
        1 attttatatg aatgattcgc ttctataaat aaaatagctt tgcgtctaac acgaaatcag
       61 tcatttaatt ggttggcaaa attataatca attgtgaagg ctgatttatt ttattgtcat
      121 aatcatattg acttatttta cataaaggaa gtttaaaaca tggatgacct aaataaaaaa
      181 tatttaatag atttacatca acatcaaaat agttcaatcg aagttttgcg tgaatttgcc
      241 gaggtaaatg aagtgccaat tgtagatcgt ttaacattag atttaattaa gcaattaatt
      301 cgtatgaata atgttaaaaa tattttagaa attggtacag caatcggcta tagttctatg
      361 caattcgctt ctatatctga tgacattcat gtcacaacga tagagcgtaa tgaaacgatg
      421 attcaatatg ctaaacaaaa tttagctact tatcattttg aaaaccaggt tcgaattatt
      481 gaaggtaatg ctttagagca atttgaaaat gtaaatgaca aagtttatga tatgatattt
      541 attgatgcag caaaagcgca atcaaagaaa ttttttgaaa tatatacacc acttttaaag
      601 caccaaggtc tcgtaattac agataatgtt ttatatcacg gttttgtatc ggatattggg
      661 attgttagat cgagaaatgt aagacaaatg gttaaaaagg ttcaagatta taatgagtgg
      721 ttaataaagc aaccaggata tacaacgaat tttttaaata tagacgatgg attagcgatt
      781 tcaattaaag gagaatgact atgacagaat tacttgtcac acccaagtca ttaagccata
      841 tggaaacact tattgattta ggtgcagatg cttttgtaat tggcgagcaa aagtttggtt
      901 taagacttcc aggggaattc aatagacaac aaatgacaga ggctgtcgca ttagctcaca
      961 aaaacgataa aaaagtatat gcggcagtga atggattatt tcataattat catttagatg
     1021 ctgtagaaga ttatattaat tttttacatg aaatacgagt tgacagaatt attttcgggg
     1081 atccagcagt agtaatgtat gtgaaggcgc aagaaaatcc aataccactt cattggaatg
     1141 cagagacgtt agtaactaat cattttcaat gtaattactg gggtaaaaga ggcgcatcaa
     1201 gagctgtctt agcacgtgag ctgaatttgg atgaaattat taatataaaa gaaaattcaa
     1261 atgtggaaat tgaggtgcaa gttcaaggaa tgacttgtat gtttcaatca aagcgtatgc
     1321 tactaggcaa ttattatacg ttccaagatc gccaaatgaa aattgaacgt cgcaatgatg
     1381 aacaatcttt attactttat gatgaagaaa gacaaaataa ttacccagtt tacgaagatt
     1441 acaatggtac gcacattatg tcaccaaacg acatttgttt aattgaagaa ttagcaccat
     1501 tttttgaggc gggtatagat tcatttaaaa tcgatggtat tctacaaacg gaagaatata
     1561 ttaatgtggt aacggaacag tatcgtcaag cgatagattt gtacaatgaa gatcctgaaa
     1621 tctatgaaga tgagaaattt atgttgatgg atccaattga agaaattcaa cctgatcatc
     1681 gaccatttga cgaaggtttc ttatataaac aaacagtata ttaaggaggt taatcatgaa
     1741 gacaatagaa gagattaaat caactcctaa aacagttatg aagaaaccag aattattagc
     1801 acctgctgga aacttagaaa agttaaaaat agcagtacat tatggcgctg atgccgtatt
     1861 tttaggtggt caagaatatg gattacgttc aaatgctgat aatttcacga tggaagaaat
     1921 agctgaaggt gttgaatttg cgaaccgtta cggtgccaaa atttatgtta cgacaaatat
     1981 tattgcacat gatgagaata ttgaaggtct agaatcatat ttgcgtaatt tggaaaagac
     2041 tggtgcgaca ggtatcattg ttgcagatcc tttaattatc gaaacatgta aagaagttgc
     2101 gccaaaactt gaaattcatt tatctactca acaatcactt tctaattaca aagctgtaga
     2161 atattggaaa gaagaaggat tggatcgtgt tgtattagca cgtgagaccg gcgcgatgga
     2221 aatgcgtgaa atgaaggaaa aagtagatat tgaaatcgaa gcatttattc atggtgctat
     2281 gtgtatcgcc tattcaggta gatgtacatt aagtaatcat atgactgcaa gggattccaa
     2341 cagaggcggt tgctgtcaaa gttgccgttg ggattatgaa ttattagaag ttgatgataa
     2401 tggtgaactt gatgtttttt ataatcaagg tgaagttaca ccgtttgcga tgagtcctaa
     2461 agatttaaaa ttaatcgaat caattcctca aatgatggat attggtgtgg actcattaaa
     2521 aattgaagga cgtatgaagt caattcatta tattgcaaca gttgtctcag tatatcgtaa
     2581 agtcattgat gcgtatgcgg cagatcctga caactttaag attaatccgg aatggttaat
     2641 agagttagat aaatgtgcaa atagagacac tgcaccagca ttctttgagg gaacacctgg
     2701 ttatgaagaa cagatgtttg gtcaacaaca atctaaaaaa tcaccatttg atttttgtgg
     2761 tttggtatta gactataatg aagatacaaa aattgcgact attcaacaac gaaataactt
     2821 taaaccaggt caagaaatag aattctttgg tccagaaatt gaaacattta cacaagtagt
     2881 agaagcaatt tatgatgaag aaggtaacag cttagacgcg gcgcgccatc cattacaaat
     2941 cgttcaaatt aaagtggatc gcccgatata tccaaacaac atgatgagaa aggaaattgg
     3001 ctaatgaaag ctactacaat cattggcata gctggtggat ctggctcagg aaaaacaact
     3061 gtaactaacg aaattatgaa aaacttagaa ggtcatagtg tcgctttact tgctcaagat
     3121 tactattata aagatcaaaa gcacttgact ttcgacgagc gcctagaaac caattatgac
     3181 catccatttg cattcgataa tgatttatta attgaaaatc ttaaagactt gaaaaatggt
     3241 aaagcagtag aagtaccgac atatgattat gctagtcata caagaagtga cattaccatt
     3301 gattttaaac ctaaagatgt tattatcgta gaaggcattt tcgctttaga aaataaggta
     3361 ttacgtgata tgatggatgt taaaatatat gttgatacag atgcagactt gagaatatta
     3421 cgccgtttaa cacgagatac taaagagcgt gggcgttcaa tggactctgt tatcaatcaa
     3481 tatttaagtg ttgttagacc tatgcatgac caatttattg aaccgactaa gaaatatgct
     3541 gatataatta ttcctgaagg tgggagcaat aaagttgcaa tagatattat gacaacaaaa
     3601 attcagtctt tagttagcaa gcaatagtat aattcagtaa aggaagatga caatatggaa
     3661 aatcaaaagc aatatccaat gactcaagaa ggttttgaaa aattagagcg tgaacttgaa
     3721 gaattaaaaa cagttaagcg tcctgaagtt gtagagaaaa ttaaagttgc acgttcattt
     3781 ggtgacttat cagagaactc tgagtatgat gcagcaaaag atgaacaagg attcatcgaa
     3841 caagatattc aaagaattga gcatatgtta agaaatgcat taatcattga agatactgga
     3901 gataacaacg ttgttaaaat tggtaaaaca gtaacgtttg tagaattacc aggtgatgaa
     3961 gaggaaagtt atcaaatcgt tggttcagct gaatcagatg catttaatgg taagatttca
     4021 aatgaatcac caatggctaa agcgttaatt ggtaaaggtt tagatgatga agttcgtgtt
     4081 ccactaccta atggtggcga aatgaacgta aaaattgtta atatccaata aatgatcaaa
     4141 tagattattt aattgcgcga ttgaagtgtg ttaatgatac ttcggtcgtg cttttttata
     4201 ttcaaaatca aaaaatcgga cagatgaaaa gtaaaaattt tccatcagac cgaatgatag
     4261 cagaacctaa attagtgtaa gagttacaat attaactact ccattttaaa aaagcaaaat
     4321 atttccgaga aaacaaattg attatcattt gttattgtat gcgatttgaa ttacttatta
     4381 aacaacttgt gcttctcaat gatacaatga aaaggtattc aatactggtt tattgcaata
     4441 caaaggggga tttaaagtgg atgtaagatt tattaacgag cagacaatta tgatttattt
     4501 tgaaaataaa atatccgaag agacatatcg taatgtcaca gcaatggtac ggtggatacg
     4561 agaaaaagaa atattagaaa ttcaagatat tgtcccttcg tatcgagcag ttctaatata
     4621 ttttgatgaa caagctataa catcttctaa attaattgaa aatttagaac ttaacaaatt
     4681 taatgaaaaa aatgttcatg ctgtgaatca aaccaatcgt attattaaaa tacctgttca
     4741 atatggtggt acatatggac cagatattga agaagtagca aagcataatc gaataactgt
     4801 tgagcaagtt attgaaaaac atacaagtaa accttattta atatatatgc taggatttat
     4861 gccaggattt ccatacttag gcggacttga tgaacaacta catacaccta gacgtaatca
     4921 gccaaggtta aaaattcatg caggttctgt aggtattgcg aataatcaaa caggactata
     4981 tccatcagat tctcctggcg gatggcaaat tattggacgc acaccgttaa aagtatttag
     5041 cagcgagcgt gaaccaatgt caatgtacga agcaggagaa tggatacaat tttatgcaat
     5101 agatgaacag aaatttatac aaatcgaaag agatatatca gatggcaact ttaatgttga
     5161 tgactgggtg gtgattgaaa atgtcaatta agattttaca accaggtctc ttttcaacgg
     5221 tacaagatct aggaagaata ggttatgaac atattggatt ttcaggcgca ggtgccatgg
     5281 atcaatttag ttttaaggtt gcgcagtcat taattaacaa tgatggtcca gcgattgaat
     5341 atactttgat tggtcctacc attcaattta atacgcaaaa tacatttgtt ataaccggtg
     5401 gtagtattaa tgcttcgcta aataataaaa ctatatcaat gaattctgtc atattagctg
     5461 agaaaggtga cattttaaaa ataggtgcta taaccaaagg tgcacgcggt tatcttactt
     5521 ttggtcattc tatcaacgta ccttctattg ctgaaagtta tgcaacacat acccgaagca
     5581 gtataggagg attcaaaggt agaaagttat tagctgatga tgtaataaca gtgaaaataa
     5641 ataatgactt taaagaaaac attggaaaga cgattcattt acaggacgat ttattgccag
     5701 aaaataatat tatacacatt cttcaaggac ctcaattcga ggcattttct gaagaggcta
     5761 gagcgaaaat tgtaaatcat ccatatttaa ttactgaaca atcagaccgt atgggctatc
     5821 gtttggaagg tgacagcgtt gcaccaatta atcaagcaga tatcatttct gaaccggttg
     5881 ctcttggtag tgttcaggta ccaaatgatg gtcaacctat tattctgctt aatgataaac
     5941 aaacgatagg tggttatacc aaaattgcaa ctgtatgcaa atttgatctg ccaaagttag
     6001 cacagatgaa accacaagat acaattcaat ttaaatggat atcattccaa gaagctgtag
     6061 ataagaatcg ggaacaaatg tctctgttta atgaaattct taagagtcat caaaagactc
     6121 caatttttga tacatctagc ttgcgacata cttcgaaaaa attagcaaca attttaaaag
     6181 gggatttgta aaatgaatat tgaaaaaatc gaacaaataa tcaaattagt gaaggaaaat
     6241 gatgttaaga aatttaaata taaaaatttt gaagatgaaa tagaaattga cttcactgac
     6301 tcgaatcatt tggctgcaca cagtaatcaa tcaaatcaaa gtatgaacaa taatgatttg
     6361 acagcttcaa aagcgaatga taactccgat gtttcgacaa atgattatca tgacattaaa
     6421 tcaccaatga taggtacatt ctttttacaa gatagtaaag aattaactga accaattgtg
     6481 aatgtcggtg acaaagttaa caagggagat attataggat atgttgaagc gatgaaagta
     6541 ttaaacgagg taacaacaga tgttgctgga gaaattactg aaatagtagc tgatcatggc
     6601 acaaatgttg aatacgacca agttttggta cgtattaagt aaagggggta agtaaatgct
     6661 tcgttgttta attgcgaaca gaggtgaaat tgctgtaagg attataagag cttgcagaga
     6721 atatgggatt gaaactgtag cagtttatgc aaaaggggac gagcaaagct tgcatgtaca
     6781 tttagcagat caagctatat gtattgggga agctaatgct ttagatagtt atttaaatat
     6841 tgaccgcatc atatctgctg cacaaatcac tggtgctaat gcaattcacc caggatatgg
     6901 ctttttatca gaatcaacaa aatttgctca aaccgttgag gaacaaggca tagcgtttat
     6961 agggcctact aaaaaaacga tggaaatgat gggggataaa ataacagcaa gacaaacagt
     7021 tcatcatgca ggtgttcctg tgattccagg atcaaatggt gctgtgaatc atgtgtctga
     7081 aattgagaat cttgccaaag acatcggtta tccagttgtt atcaaagctg ccagtggcgg
     7141 tggtggtaaa ggtatacgta ttgtaaagaa agctgaagat ttagaaaagg catttaaaga
     7201 agctaaaagt gaaggaaaaa aatactttga tgatgatcgt gtttatgttg aggcttttat
     7261 acctgttgca aaacatgttg aagttcaagt tatgggagat gggcaggata attatgtaca
     7321 tttaggggaa cgtgattgtt ctgtacaacg taaaaatcaa aaattaatcg aagaatctcc
     7381 ttgtgcagca ttaactgaag aaagacgaca acaaatatgt aacgatgctg ttaaagttgc
     7441 acgtgcagca aattatagaa gtgcaggtac gattgaattt ttagtaactg ataccgcaca
     7501 ttactttatt gaaatgaatg ctcgaataca agttgaacat actgttacag agatgcgagc
     7561 agaacgtgat ttggttgcag cacaactgta tttattggaa cataatcatt tacctttttc
     7621 acaaagcgat attcaattta atggtcatgt gatagaagcg agaataaatg ccgaaaatcc
     7681 tgaaaagaaa tttcaaccaa caccaggtaa agtgacagcg ttacatttac ctcaaggctt
     7741 taatgtgcga gtcgattctt tactatatca cggttatcaa gtttcaccat attatgattc
     7801 tttagtagct aaagtaattg taaaatctca tgatagagct tcagcaattg ataagttgaa
     7861 agtcacctta gatgaaatgg tgatagatgg attttctaca actgccgatt ttttatatgc
     7921 agttttaaat tatccactat atcgtgatgg agatgcaaaa gatgttgata ttaaattttt
     7981 agaaaaacat caaattgtta aaggggtgga attataatgc gagttgattt gaattgtgat
     8041 ttaggcgaag catttggaaa ttattccttt ggtggtgatc atcaaattat tccgttaatt
     8101 acaagtgcga atgttgcttg tggttttcac gctggtgatg aaaatgtaat gaatgaaacg
     8161 gtaaaacttg ccaaagcaca taatgttgca gtaggtgcac atcctggttt acctgatttg
     8221 aaaggctttg gcagacgaaa tatagatatc tctaacgacg agatttataa tttgatgatt
     8281 tatcaattag gtgcattaca agggttttgt cgcattcatc aagttaaaat taatcatgtt
     8341 aaaccgcatg gtgcattgta tcagatgggt gcaaaagaca gagaaatagc aaacgttata
     8401 gcacaagctg tttatgactt tgatccatca ctagtgttag taggattagc aaattcatat
     8461 ctaatttcag aagcaaagaa tgtcggatta attacagctt ctgaagtgtt tgctgataga
     8521 cgatacgaag atgatgggca gctcgttagt agaaaagaaa gtgatgctgt gattactgat
     8581 actgacgaag cacttaagca ggttttaaag atggtgaagg aaaataaagt tatttcaaaa
     8641 aacaataagg aagtaacgtt acaagcagat acaatttgtg tgcatggtga tggagaacat
     8701 gcattattat ttgtttcgaa aattagagaa attttaatga aagaaggcat tgatattcaa
     8761 tccttatagg gggctaaaaa aatggggaga aatttaaagc ttaaaaaaga aagtgacttt
     8821 gaattcacaa aaaatcataa aaggttatta ttaggttctg tatttttgat ggcaacttct
     8881 gcaattgggc cagcattttt aacgcaaaca gcagtattta catcacaatt ttttgcaagt
     8941 ttcgcatttg ccatattact gtctatcatc attgacattg gtgcacaaat taatatatgg
     9001 cgcatattag ttgtaactgg tttaagaggt caagaaatat caaataaagt tgttcctggg
     9061 cttggtactg ttatctcaat attgattgca tttggtggtc tagcttttaa tattggtaat
     9121 attgctggtg ccggtttagg tttaaatgca atttttggat tagatgtaaa atggggcgca
     9181 gctattactg caatctttgc aatattaatc tttgtaagta aaagtggcca aaaaattatg
     9241 gacgttgttt caatgattct tggtattgtg atgattttag ttgtggcata tgtgatgttt
     9301 gtttctaatc caccttatgg tgatgctttt gtgcatacat ttgcgccaga acatccaatg
     9361 aaattagtct tgcccatcat tacgttagtt ggtggaactg taggtggtta tattaccttt
     9421 gcaggtgcac atcgtatatt agactctggc attaaaggta agcaatattt accatttgta
     9481 aatcaatcag caattgctgg tattttaact acaggtatta tgagaacgtt actattccta
     9541 gcggtattag gagttgttgt aacaggtgtg acactaagtt ctgaaaatcc accagcgtca
     9601 gtttttgaac acgcaattgg accaattgga aagaatattt ttggtattgt gttatttgct
     9661 gcagctatgt catcagtaat tggctcagca tacacaagcg caacattttt aaaaacactt
     9721 cataaatcac ttaacgaaag aagtaattta attgtgattg tgtttatcgt tatttcaaca
     9781 atgattttct tatttattgg aaaaccaatc agccttttaa ttatagcagg cgcgataaat
     9841 ggatggattt tacctattac gttaggtgca attttgattg caagtaaaaa gaaatcaatt
     9901 gttggtgatt ataaacaccc aaattggatg ttcatctttg gtatcgttgc agtacttgtt
     9961 acaatattga caggtatatt ctcattcaaa gaagtacttc agttatttta aaagacattt
    10021 cgactaatag catcactgta aatgttgtta gtattaggca agatatattt tgatttccgg
    10081 tgattcattt ataaagcata attagatata ttttaaggga gagtggtgca tgacaaatgt
    10141 actgctcttt ttgttgtgat gaaaaatctt tttctattga ttgagaaaat agttatttat
    10201 atagttttta aagaaaaaaa gtgataatat ttagctaatc taatgaaaat tgatttattg
    10261 gacaaaaaat acattttaaa tgagtagagg aggctgcaaa tgggttacag aactttaaaa
    10321 agtatttttc atgaacacaa tgaaagtaaa atgaaggaag agtatattaa aagatttaat
    10381 tctttagctt ctttcaatac taatattaat atcataccta tggaaaatgg aaaaaaagtt
    10441 aatgatttgg aatatcctct attctttatg gtgactaaaa atctatcaaa aaaacaagaa
    10501 ttaatatcaa ttaatagtag aaaaattgat agagcactta attctttacc atatgcagct
    10561 agagaacaat attttaatga tttattaatc gatgaattac aaagtactaa tgaaattgaa
    10621 aatgtattta gtactaaaca agagattgca catgcgttaa ataaccaagc atcagaattt
    10681 cttaagttca gaggcctcgt ggatcaatat aaagagatag aacttaataa aaaaattaaa
    10741 gttgataatg taagagacat tagagcgatt tatgataaat tagtttcaaa tgaaattaac
    10801 gaacaagata agttagatgg agagctattt cgtaaaaatt ttgtcggtgt gcatgatggg
    10861 tcaacgaata aatatataca tgttgggtta caacctgaaa ccaaaattgt tgaatttata
    10921 ggtgaaatgc taacattttt aaaatatttt gatgcgcctc agccgttcaa aatcatggct
    10981 agtcattatc tgtttgaata tatacatcct ttttatgatg gcaatggtag agttggaaga
    11041 tttataattg ccaagctctt gagtgattat tatgataatt atactgcttt gacattttca
    11101 tatgttataa atagaaataa atcgaaatat tacaaggcgt ttatgactgc ttcaaatcat
    11161 ttgaattgtg gtgatttgac ggaatttatt gacacaatgc ttgagctatt gatagcagga
    11221 caagaaagaa tacttgatga gttaatacca aaaatggatg cgactgaaaa attaacgctt
    11281 tatttaacta gtcattataa acagatagat tatgaatttt tgtatctcct ttcaatggat
    11341 aaattgtttg gaaataaaag aaatagatta acattaattg atttagaaaa catat
//
LOCUS       DALAGD010000092        11327 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.093, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000092 DALAGD010000000
VERSION     DALAGD010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11327
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.093"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            262..1950
                     /gene="ilvD"
                     /locus_tag="O2701_002171"
     CDS             262..1950
                     /gene="ilvD"
                     /locus_tag="O2701_002171"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCZ4294050.1"
                     /translation="MRSDMIKKGDHQAPARSLLHATGALKSPTDMNKPFVAICNSYID
                     IVPGHVHLRELADIAKEAIREAGAIPFEFNTIGVDDGIAMGHIGMRYSLPSREIIADA
                     AETVINAHWFDGVFYIPNCDKITPGMILAAMRTNVPAIFCSGGPMKAGLSAHGKALTL
                     SSMFEAVGAFKEGSISKEEFLDMEQNACPTCGSCAGMFTANSMNCLMEVLGLTLPYNG
                     TALAVSDQRREMIRQAAFKLVENIKNDLKPRDIVTREAIDDAFALDMAMGGSTNTVLH
                     TLAIANEAGIDYDLERINAIAKRTPYLSKIAPSSSYSMHDVHEAGGVPAIINELMKKD
                     GTLHPDRITVTGKTLRENNEGKEIKNFDVIHPLDAPYDAQGGLSILFGNIAPKGAVIK
                     VGGVDPSIKTFTGKAICFNSHDEAVEAIDNRTVRAGHVVVIRYEGPKGGPGMPEMLAP
                     TSSIVGRGLGKDVALITDGRFSGATRGIAVGHISPEAASGGPIALIEDGDEITIDLTN
                     RTLNVNQPEDVLARRRESLTPFKAKVKTGYLARYTALVTSANTGGVMQVPENLI"
     gene            1978..3747
                     /gene="ilvB"
                     /locus_tag="O2701_002172"
     CDS             1978..3747
                     /gene="ilvB"
                     /locus_tag="O2701_002172"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HCZ4294051.1"
                     /translation="MSKTQHEVNQNIDPLKMAESLEPEQLNEKTLNDMRSGSEVLVEA
                     LLKENVDYLFGYPGGAVLPLYDTFYDGKIKHILARHEQGAVHAAEGYARVSGKTGVVV
                     VTSGPGATNVMTGITDAHCDSLPLVVFTGQVATPGIGKDAFQEADILSMTSPITKQNY
                     QVKRVEDIPKIVHEAFHVANSGRKGPVVIDFPKDMGVLATNVDLCDEINIPGYEVVTE
                     PENKDIDTFISLLKEAKKPVVLAGAGINQSKSNQLLTQFVNKHQIPTVTTLLGLGAVP
                     YEDTLFLGMGGMHGSYASNMALTECDLLINLGSRFDDRLASKPDAFAPNAKIVHVDID
                     PSEINKVIHVDLGIIADCKRFLECLNDKNVETIEHSDWVKHCQNNKQKHPFKLGEEDQ
                     VFCKPQQTIEYIGKITNGEAIVTTDVGQHQMWAAQFYPFKNHGQWVTSGGLGTMGFGI
                     PSSIGAKLANPDKTVVCFVGDGGFQMTNQEMALLPEYGLDVKIVLINNGTLGMVKQWQ
                     DKFFNQRFSHSVFNGQPDFMKMAEAYGVKGFLIDKPEQLEEQLDAAFAYQGPALIEVR
                     ISPTEAVTPMVPSGKSNHEMEGL"
     gene            3747..4001
                     /locus_tag="O2701_002173"
     CDS             3747..4001
                     /locus_tag="O2701_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HCZ4294052.1"
                     /translation="MTRILKLQVADQVSTLNRITSAFVRLQYNIDTLHVTHSEQPGIS
                     NMEIQVDIQDDTSLHILIKKLKQQINVLTVECYDLVDNEA"
     gene            4138..5142
                     /gene="ilvC"
                     /locus_tag="O2701_002174"
     CDS             4138..5142
                     /gene="ilvC"
                     /locus_tag="O2701_002174"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCZ4294053.1"
                     /translation="MTTVYYDQDVKTDALQGKKIAVVGYGSQGHAHAQNLKDNGYDVV
                     IGIRPGRSFDKAKEDGFDVFPVAEAVKQADVIMVLLPDEIQGDVYKNEIEPNLEKHNA
                     LAFAHGFNIHFGVIQPPADVDVFLVAPKGPGHLVRRTFVEGSAVPSLFGIQQGASGQA
                     RNIALSYAKGIGATRAGVIETTFKEETETDLFGEQAVLCGGVSKLIQSGFETLVEAGY
                     QPELAYFEVLHEMKLIVDLMYEGGMENVRYSISNTAEFGDYVSGPRVITPDVKENMKA
                     VLTDIQNGNFSNRFIEDNKNGFKEFYKLREEQHGHQIEKVGRELREMMPFIKSKSIEK
                     "
     gene            5172..6701
                     /locus_tag="O2701_002175"
     CDS             5172..6701
                     /locus_tag="O2701_002175"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCZ4294054.1"
                     /translation="MSSHIQIFDTTLRDGEQTPGVNFTFDERLRIALQLEKWGVDVIE
                     AGFPASSTGSFKSVQAIAQTLTTTAVCGLARCKKSDIDAVYEATKDAAKPVVHVFIAT
                     SPIHLEHKLKMSQEDVLASIKEHVTYAKQLFDVVQFSPEDATRTELPFLVKCVQTAVD
                     AGATVINIPDTVGYSYHDEYAHIFKTLTESVTSSNEIIYSAHCHDDLGMAVSNSLAAI
                     EGGARRIEGTVNGIGERAGNAALEEVALALYVRNDHYGAQTALNLEETKKTSDLISRY
                     AGIRVPRNKAIVGQNAFSHESGIHQDGVLKHRETYEIMTPQLVGVSTTELPLGKLSGK
                     HAFSEKLKALGYDIDKEAQIDLFKQFKAIADKKKSVSDRDIHAIIQGSEHEHQALYKL
                     ETLQLQYVSSGLQSAVVVVKDKEGHIYQDSSIGTGSIVAIYNAVDRIFQKETELIDYR
                     INSVTEGTDAQAEVHVNLLIEGKTVNGFGIDHDILQASCKAYVEAHAKFAAENVEKVG
                     N"
     gene            6704..7750
                     /gene="leuB"
                     /locus_tag="O2701_002176"
     CDS             6704..7750
                     /gene="leuB"
                     /locus_tag="O2701_002176"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCZ4294055.1"
                     /translation="MTYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFG
                     GASIDTFGEPLTEKTLNACKRADAILLGAIGGPKWTDPNNRPEQGLLKLRKSLNLFVN
                     IRPTTVVKGASSLSPLKEERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTRE
                     EIERIVHVAFKLAASRRGKLTSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDA
                     CSMHLITNPKQFDVIVCENLFGDILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGS
                     APDIAGKNVANPFGMILSLAMCLRESLNQPDAADELEQHIYSMIEHGQTTADLGGKLN
                     TTDIFEILSQKLNH"
     gene            7764..9134
                     /gene="leuC"
                     /locus_tag="O2701_002177"
     CDS             7764..9134
                     /gene="leuC"
                     /locus_tag="O2701_002177"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCZ4294056.1"
                     /translation="MGQTLFDKVWNRHVLYGKLGEPQLLYIDLHLIHEVTSPQAFEGL
                     RLQNRKLRRPDLTFATLDHNVPTIDIFNIKDEIANKQITTLQKNAIDFGVHIFDMGSD
                     EQGIVHMVGPETGLTQPGKTIVCGDSHTATHGAFGAIAFGIGTSEVEHVFATQTLWQT
                     KPKNLKIDINGTLPTGVYAKDIILHLIKTYGVDFGTGYALEFTGETIKNLSMDGRMTI
                     CNMAIEGGAKYGIIQPDDITFEYVKGRPFADNFAKSVDKWRELYSDDDAIFDRVIELD
                     VSTLEPQVTWGTNPEMGVNFSEPFPEINDINDQRAYDYMGLEPGQKAEDIDLGYVFLG
                     SCTNARLSDLIEASHIVKGNKVHPNITAIVVPGSRTVKKEAEKLGLDTIFKNAGFEWR
                     EPGCSMCLGMNPDQVPEGVHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAIHGKFVD
                     VRKVVV"
     gene            9135..9707
                     /gene="leuD"
                     /locus_tag="O2701_002178"
     CDS             9135..9707
                     /gene="leuD"
                     /locus_tag="O2701_002178"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCZ4294057.1"
                     /translation="MAAIKPITTYKGKIVPLFNDNIDTDQIIPKVHLKRISKSGFGPF
                     AFDEWRYLPDGSDNPDFNPNKPQYKGASILITGDNFGCGSSREHAAWALKDYGFHIII
                     AGSFSDIFYMNCTKNAMLPIVLEKSAREHLAQYVEIEVDLPNQTVSSPDKRFHFEIDE
                     TWKNKLVNGLDDIAITLQYESLIEKYEKSL"
     gene            9722..10990
                     /gene="ilvA"
                     /locus_tag="O2701_002179"
     CDS             9722..10990
                     /gene="ilvA"
                     /locus_tag="O2701_002179"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HCZ4294058.1"
                     /translation="MTVKTTVSTKDIDEAFLRLKDIVKETPLQLDHYLSQKYDCKVYL
                     KREDLQWVRSFKLRGAYNAISVLSDEAKSKGITCASAGNHAQGVAYTAKKLNLNAVIF
                     MPVTTPLQKVNQVKFFGNSNVEVVLTGDTFDHCLAEALTYTSEHQMNFIDPFNNVHTI
                     SGQGTLAKEMLEQAKSDNVNFDYLFAAIGGGGLISGISTYFKTYSPTTKIIGVEPSGA
                     SSMYESVVVNNQVVTLPNIDKFVDGASVARVGDITFEIAKENVDDYVQVDEGAVCSTI
                     LDMYSKQAIVAEPAGALSVSALENYKDHIKGKTVVCVISGGNNDINRMKEIEERSLLY
                     EEMKHYFILNFPQRPGALREFVNDVLGPQDDITKFEYLKKSSQNTGTVIIGIQLKDHD
                     DLIQLKQRVNHFDPSNIYINENKMLYSLLI"
     gene            complement(11200..11314)
                     /gene="rrf"
                     /locus_tag="O2701_002180"
     rRNA            complement(11200..11314)
                     /gene="rrf"
                     /locus_tag="O2701_002180"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         3807 a         1881 c         2199 g         3440 t
ORIGIN      
        1 ttcacggaag gagtataata aaatgcttaa tcaatatact gaacatcaac cgacaacttc
       61 aaatattatt attttattat actctttagg actcgaacgt tagtaaatat ttactaaacg
      121 ctttaagtcc tatttctgtt tgaatgggac ttgtaaacgt cccaataata ttgggacgtt
      181 tttttatgtt ttatctttca attacttatt tttattacta taaaacatga ttaatcatta
      241 aaatttacgg gggaatttac tatgcgaagc gacatgatca aaaaaggaga tcaccaagca
      301 ccagcaagaa gtcttttaca tgccacgggc gcgctaaaaa gtccaactga tatgaacaaa
      361 ccatttgtag ctatttgtaa ctcttatatt gatattgttc ctggacatgt tcacttgaga
      421 gagcttgcag atatagctaa agaagcaatt agagaagccg gtgccattcc atttgaattc
      481 aatacaattg gtgttgatga tggaatagct atgggacata tcggaatgcg atattctcta
      541 ccatcacgtg aaattattgc agatgcagct gaaactgtaa ttaacgctca ttggtttgac
      601 ggcgtatttt acattcctaa ttgtgacaag attacacccg gtatgatttt agcagccatg
      661 aggacaaacg taccagctat cttttgctct ggtggaccaa tgaaagctgg cttatctgca
      721 catggaaaag cattaacact ttcatcaatg tttgaagcag tcggcgcatt taaagaagga
      781 tcgatttcta aagaagaatt tttagatatg gaacaaaatg cctgccctac ttgtggttca
      841 tgtgctggga tgtttactgc aaattcaatg aactgtttga tggaagtttt aggtctaaca
      901 ttaccataca acggtactgc acttgcagtc agtgatcagc gacgagaaat gattcgccaa
      961 gcagctttta aattagttga aaatattaaa aatgatttaa aaccacgtga tatcgttact
     1021 cgcgaagcaa ttgatgatgc atttgcactt gatatggcta tgggtggttc aacaaacacg
     1081 gtactgcata cgttagccat tgccaatgaa gctggtattg attatgactt agagcgcatt
     1141 aatgctattg ccaaacgcac gccatattta tcaaaaatag cacctagttc atcgtattca
     1201 atgcatgatg tgcatgaagc tggtggcgtc ccagcaatta ttaatgaatt gatgaagaaa
     1261 gatggcacgt tacacccaga tagaatcaca gttactggca aaacgttacg tgaaaataac
     1321 gaaggcaaag aaattaagaa ctttgatgtc attcaccctc ttgatgcacc atatgatgca
     1381 caaggcggtt tatctatctt atttggtaat atcgccccta aaggcgcagt tattaaagtt
     1441 ggcggcgttg atccatctat caaaacattt actgggaaag caatttgttt caattcgcat
     1501 gatgaagctg ttgaagcaat agacaatcgt accgttcgtg caggccacgt cgttgtcatt
     1561 agatatgaag gacctaaagg tggaccaggt atgcctgaaa tgttagcacc tacttcctct
     1621 attgttggtc gcggcttagg taaagatgtt gcattaatta ctgatgggcg tttttccggt
     1681 gccacaagag gtattgcagt tggtcatatt tcccctgaag ctgcatctgg tggaccaatt
     1741 gccttaattg aagatggtga tgagattact attgatttaa caaatcgtac attaaacgta
     1801 aaccagcctg aagatgttct agcgcgtcgc cgagaatctt taacaccatt taaagcgaaa
     1861 gtaaaaacag gttatctagc tcgttatact gccctagtaa ctagcgcaaa tacaggtggc
     1921 gtcatgcaag tccctgagaa tttaatttaa tttattttta tattggagat ggttaaaatg
     1981 tctaaaactc aacatgaagt aaaccaaaat attgaccctt taaaaatggc tgaatcactt
     2041 gaacctgaac aactaaatga aaaaacttta aatgatatgc gttcaggatc agaagtgcta
     2101 gtagaagctc tacttaaaga aaatgtggat tatttattcg gttatcctgg tggtgccgta
     2161 ctacctttat atgacacgtt ttatgatggt aaaatcaaac atattttagc aagacacgaa
     2221 caaggtgctg ttcatgctgc agaaggttat gcacgtgtat ctggtaaaac tggcgtcgtt
     2281 gtagttacaa gcggtccagg tgcaactaat gtaatgacag gtattacgga tgcacattgc
     2341 gactctttac ctctagttgt attcactgga caagttgcta caccaggcat tggtaaagat
     2401 gcattccaag aagcggatat tctatctatg acttcaccaa ttacaaaaca aaattatcaa
     2461 gtgaaacgtg ttgaagatat ccctaaaatc gtacacgaag ctttccatgt agctaattct
     2521 ggacgcaaag gtcctgtagt gattgatttt ccaaaagata tgggtgtttt agctacaaat
     2581 gtggatttat gcgacgaaat caatattcca ggttatgaag ttgttacaga accagaaaat
     2641 aaagacattg acactttcat ctcactttta aaagaagcga aaaagcctgt cgtattagcc
     2701 ggcgcaggta ttaatcaatc aaaatcaaat caattattaa cacagtttgt taataaacat
     2761 caaatcccaa cagttactac tttactaggt ctaggtgctg taccttatga ggatacacta
     2821 tttttaggta tgggaggaat gcatggttct tatgctagta acatggcatt aactgagtgt
     2881 gatttactca ttaatttagg tagccgcttc gatgatagat tagcaagcaa acctgatgcc
     2941 tttgcaccta acgccaaaat tgtacatgta gatattgatc cttcagaaat caataaagtt
     3001 attcatgtag atttaggtat tattgcagac tgtaaaagat ttttagaatg tttaaatgat
     3061 aaaaatgttg agactataga acacagtgac tgggttaaac attgtcaaaa taataagcag
     3121 aaacacccat ttaaacttgg tgaagaagat caagtatttt gtaagccaca acaaacaatc
     3181 gaatatatcg gcaaaattac aaatggtgaa gcaattgtta ctacagacgt gggacaacat
     3241 caaatgtggg cagctcaatt ttatccattt aaaaatcacg gacaatgggt tacaagcggt
     3301 ggtttaggaa caatgggatt cggtattcct tcgtcaattg gtgccaaatt agctaatcct
     3361 gataaaacag tcgtatgttt cgtcggtgac ggtggtttcc aaatgacaaa ccaagaaatg
     3421 gcacttttac ccgaatatgg tttagatgtc aaaatcgtac taatcaataa tggaacatta
     3481 ggtatggtta aacaatggca agataagttc tttaatcaac gcttctcaca ctcagtattt
     3541 aatggtcaac ctgattttat gaaaatggca gaagcatatg gcgtcaaagg tttcttaatc
     3601 gataagccag aacaactgga agaacaatta gatgcagcgt ttgcttatca aggaccagct
     3661 ttaattgagg ttcgtatttc ccctactgaa gctgtaaccc caatggttcc gagtggcaaa
     3721 tcaaatcatg aaatggaggg cttataatga caagaattct taaattacaa gttgcggatc
     3781 aagtcagcac gctaaatcga attacaagtg cttttgttcg cctacaatat aatatcgata
     3841 cattacatgt tacacattct gaacaacctg ggatttctaa catggaaatt caagtcgata
     3901 ttcaagatga tacatcactt catatattaa ttaaaaaatt aaaacaacaa attaatgttt
     3961 taacggttga atgctacgac cttgttgata acgaagctta attttaagac aaaggcaatg
     4021 atgcgctaat tagttataga tatatcatag gctgctagtt aacatctgcc actattacaa
     4081 agttatattt cagaattttc gaaacacaaa atatttaatt atttggagga atttattatg
     4141 acaacagttt attatgatca agatgtaaaa acggacgctt tacaaggcaa aaaaattgca
     4201 gtagtaggtt atggatcaca aggtcacgcg catgcacaaa acttaaaaga caatggatat
     4261 gatgtagtca tcggcattcg cccaggtcgt tcttttgaca aagctaaaga agatggattt
     4321 gatgtgttcc ctgttgcaga agcagttaag caagctgatg taattatggt gctattacct
     4381 gatgaaattc aaggtgatgt atacaaaaac gaaattgaac caaatttaga aaaacataat
     4441 gcgcttgcat ttgctcatgg ctttaacatt cattttggtg ttattcaacc accagctgat
     4501 gttgatgtat ttttagtagc tcctaaagga ccgggtcatt tagttagacg tacatttgtt
     4561 gaaggttctg ctgtaccatc actatttggt attcaacaag gcgcttcagg tcaagcacgt
     4621 aatattgctt taagttatgc aaaaggtatt ggtgcaactc gtgcaggtgt tattgaaaca
     4681 acatttaaag aagaaactga gacagattta tttggtgaac aagcagtact ttgcggtggt
     4741 gtatcgaaat taattcaaag tggctttgaa acattagtag aagcgggtta tcaaccagaa
     4801 ttagcttatt ttgaagtatt acatgaaatg aaattaatcg ttgatttgat gtatgaaggc
     4861 ggtatggaaa atgtacgtta ctcaatttca aatactgctg aatttggtga ctatgtttca
     4921 ggaccacgtg ttatcacacc agatgttaaa gaaaatatga aagctgtatt aactgatatc
     4981 caaaatggta acttcagtaa tcgctttatc gaagacaata aaaatggatt caaagaattt
     5041 tataaattac gcgaagaaca acatggtcat caaattgaaa aagttggtcg tgaattacgc
     5101 gaaatgatgc cttttattaa atctaaaagc attgaaaaat aagatagacc tacaatgagg
     5161 agttgttaaa tatgagtagt catattcaaa tttttgatac gacactaaga gacggtgaac
     5221 aaacaccagg agtgaatttt acttttgatg aacgcttgcg tattgcattg caattagaaa
     5281 aatggggtgt agatgttatt gaagctggat ttcctgcttc aagtacaggt agctttaaat
     5341 ctgttcaagc aattgcacaa acattaacaa caacggctgt atgtggttta gctagatgta
     5401 aaaaatctga catcgatgct gtatatgaag caacaaaaga tgcagcgaag ccggtcgtgc
     5461 atgtttttat agcaacatca cctattcatc ttgaacataa acttaaaatg tctcaagaag
     5521 acgttttagc atctattaaa gaacatgtca catacgcgaa acaattattt gacgttgttc
     5581 aattttcacc tgaagatgca acgcgtactg aattaccatt cttagtgaaa tgtgtacaaa
     5641 ctgccgttga cgctggagct acagttatta atattcctga tacagtcggc tacagttacc
     5701 atgatgaata tgcacatatt ttcaaaacct taacagaatc tgtaacatct tcaaatgaaa
     5761 ttatttatag tgctcattgc catgacgatt taggaatggc tgtttcaaat agtttagctg
     5821 caattgaagg cggtgcgaga cgaattgaag gcactgtaaa tggtattggt gaacgagcag
     5881 gtaatgcagc acttgaagaa gtcgcgcttg cactatacgt tcgaaatgat cattatggtg
     5941 ctcaaactgc tcttaatctc gaagaaacta aaaaaacatc ggatttaatt tcaagatatg
     6001 caggtattcg agtgcctaga aataaagcaa ttgttggcca aaatgcattt agtcatgaat
     6061 caggtattca ccaagatggc gtattaaaac atcgtgaaac atatgaaatt atgacacctc
     6121 aacttgttgg tgtaagcacg actgaacttc cattaggaaa attatctggt aaacacgcct
     6181 tctcagagaa gttaaaagca ttaggttatg acattgataa agaagcgcaa atagatttat
     6241 ttaaacaatt caaggccatt gcggacaaaa agaaatctgt ttcagataga gatattcatg
     6301 cgattattca aggttctgag catgagcatc aagcacttta taaattggaa acactgcaac
     6361 tacaatatgt ctctagcggc cttcaaagtg ctgttgttgt tgttaaagat aaagagggtc
     6421 atatttacca ggattcaagt attggtactg gttcaatcgt agcaatttac aatgcagttg
     6481 atcgtatttt ccagaaagaa acagaattaa ttgattatcg tattaattct gtcactgaag
     6541 gtactgatgc ccaagcagaa gtacatgtaa atttattgat tgaaggtaag actgtcaatg
     6601 gctttggtat tgatcatgat attttacaag cctcttgtaa agcatacgta gaagcacatg
     6661 ctaaatttgc agctgaaaat gttgagaagg taggtaatta attatgactt ataacattgt
     6721 tgccctacct ggtgatggaa tcggtccaga aattttgaac ggatctctat cattgcttga
     6781 aattataagt aataaatata actttaatta tcaaatagag caccacgaat ttggtggtgc
     6841 ctctattgat acattcggcg agcctttaac tgagaaaacc ttaaatgcgt gtaaaagagc
     6901 agatgctatt ttactgggtg caatcggtgg acctaaatgg acagatccta acaatcgacc
     6961 agaacaagga ttattaaaat tgcgtaaatc cttaaattta tttgtaaata tacgccccac
     7021 taccgttgtc aaaggcgcta gttctttatc acctttaaag gaagaacgcg ttgaaggcac
     7081 agatttagtt atagtccgtg aattgacaag tggtatttat tttggagaac ctagacattt
     7141 taataatcac gaggccttag attctcttac ttatacaaga gaagaaatag aacgcattgt
     7201 tcacgtagca tttaaattgg ccgcttcaag acgaggaaaa ctaacatcag ttgataaaga
     7261 aaatgtatta gcttctagta aattgtggcg caaagtcgta aatgaagtaa gtcaattata
     7321 tccagaagta acagtaaatc acttacttgt tgatgcttgt agtatgcatt taatcacaaa
     7381 tccaaaacaa tttgacgtca tcgtatgtga aaacttattt ggcgatattt taagtgatga
     7441 agcttcagtg attcctggtt cacttggttt atcaccttct gctagtttta gtaacgatgg
     7501 tccaagattg tatgagccta ttcatggatc agcaccagat attgcaggta aaaacgttgc
     7561 caatccattt ggaatgattc tatctttagc gatgtgttta cgtgaaagct taaatcaacc
     7621 agatgctgca gatgaattag aacaacatat ttatagcatg attgaacatg ggcaaacgac
     7681 agcagattta ggcggcaaat tgaatactac tgatattttc gaaattctat ctcaaaaatt
     7741 gaatcactaa gggggagatg taaatgggtc aaacattatt tgacaaggtg tggaacagac
     7801 atgtgttata cgggaaattg ggcgaaccgc aactattata cattgattta caccttatac
     7861 atgaagttac ttctcctcaa gcatttgaag gacttaggct tcaaaacaga aaattaagac
     7921 gcccagattt aacatttgca acactcgatc acaatgttcc tactattgat atattcaata
     7981 ttaaagatga aattgcaaac aaacaaatca caacattaca aaaaaacgcc atagattttg
     8041 gggtgcatat ttttgatatg ggttctgatg aacaaggtat tgttcacatg gtaggacctg
     8101 agacaggact tacacagcct ggcaagacaa tcgtttgtgg tgactctcac acagcaacac
     8161 atggagcatt tggtgctatt gcatttggaa ttggaacaag tgaagttgaa catgttttcg
     8221 caactcaaac gctatggcaa acaaaaccca aaaacttaaa aatcgatatt aatggtacct
     8281 taccaacagg cgtctatgct aaggacatta ttctgcattt aattaaaacg tatggtgttg
     8341 actttggtac aggctatgct ttggaattta ctggcgaaac aattaaaaac ctttcaatgg
     8401 atggtcgaat gactatttgt aacatggcta tcgaaggtgg tgccaaatac ggcataatcc
     8461 aacctgatga tataacattt gaatatgtta aagggagacc atttgccgat aacttcgcta
     8521 aatcagttga taagtggcgt gagctatatt ctgatgacga cgcgatattt gatcgtgtaa
     8581 ttgaacttga tgtttcaaca ttagaaccac aagtgacatg gggaactaat cctgaaatgg
     8641 gtgttaattt cagtgaacca ttccctgaaa tcaatgatat caacgatcaa cgtgcgtatg
     8701 attatatggg gttagaacca ggtcaaaaag ctgaagacat cgacttaggg tatgtttttc
     8761 tcggttcatg tacaaatgct agactatcag atttgattga agctagtcat attgttaaag
     8821 gaaataaagt tcatccaaat attacagcta ttgtcgtacc aggttctcgt acagtaaaaa
     8881 aagaagcaga aaaattaggt ctagatacta tctttaaaaa tgcaggattt gaatggcgtg
     8941 aaccaggatg ttcaatgtgt ttaggcatga atcctgacca agtacctgag ggcgtacatt
     9001 gtgcatctac aagtaatcga aactttgaag gacgacaagg caaaggtgca agaacacatt
     9061 tagtatcccc tgctatggca gcagcagcag ctattcatgg taaatttgtg gacgtaagaa
     9121 aggtggttgt ttaaatggca gcaatcaaac ctattacaac atataaaggt aaaatagtcc
     9181 ctctcttcaa cgacaatatc gatacagacc aaatcattcc taaggtacac ttaaagcgta
     9241 tttcaaaaag tggctttggt ccatttgctt ttgatgaatg gcggtactta cctgatggtt
     9301 cagataatcc tgatttcaat cctaacaaac cacaatataa aggggcttct attttaatta
     9361 ctggagataa ttttggatgt ggttcaagtc gtgaacatgc tgcttgggct cttaaggact
     9421 atggttttca tattattatt gcaggaagtt tcagtgacat attttatatg aattgcacta
     9481 aaaatgcgat gttgcctatc gttttagaaa aaagtgcccg tgaacatctt gcacaatatg
     9541 ttgaaattga ggtcgattta ccaaatcaaa ctgtgtcatc accagacaag cgtttccatt
     9601 ttgaaattga tgaaacttgg aagaataaac ttgtaaatgg cttagatgac attgcaatca
     9661 ccctacaata tgaatcatta atagaaaaat atgaaaaatc actttaaggg agttgaatat
     9721 tatgacagtc aaaacaacag tttctacgaa agatatcgat gaggcatttt taagacttaa
     9781 agatattgtc aaagaaacac ctttacaatt agaccattac ttatctcaaa agtatgattg
     9841 taaagtctat ttaaaacgag aagatttaca atgggtacgt tcttttaaat taagaggtgc
     9901 ttacaacgct atttctgttt tatcagatga agctaaaagt aaaggtatta catgtgcaag
     9961 tgcaggtaat catgctcaag gtgttgccta tacagctaaa aaacttaatt taaacgctgt
    10021 tatctttatg ccagtcacta cacctttaca aaaggtaaat caagtaaagt tctttggaaa
    10081 tagtaacgtt gaagttgtac tcactggtga tacatttgat cactgtttag ctgaagcttt
    10141 aacttataca agtgaacatc aaatgaactt tatagatcca ttcaataatg ttcatacaat
    10201 ttctggacaa ggtacgcttg ctaaagaaat gctagaacaa gcaaagtctg acaatgttaa
    10261 ctttgattat ctatttgccg caattggtgg tggcggttta atttcaggta ttagtactta
    10321 ctttaaaacc tattcaccta ccacgaaaat tataggtgtt gaaccttcag gtgcaagtag
    10381 tatgtatgaa tctgttgtgg taaataatca ggtagtcaca ttgcctaata tcgataaatt
    10441 tgtggacggt gcatctgtag ctagagttgg cgatattaca tttgaaattg caaaagaaaa
    10501 tgtagatgat tacgttcaag tagatgaagg tgcagtttgt tctacgattt tagatatgta
    10561 ttcaaaacaa gcaattgtag cagaacctgc tggcgcatta agtgtaagtg cgcttgaaaa
    10621 ctataaagat catattaaag gtaaaacagt ggtttgtgtc attagtggtg gtaataatga
    10681 tattaatcga atgaaagaaa ttgaagaacg ttcattacta tacgaagaaa tgaagcatta
    10741 ctttatctta aatttccctc aacgtccagg tgcattgaga gaatttgtaa atgacgtatt
    10801 aggacctcaa gacgatatta ctaaatttga atacttaaaa aaatcttctc aaaatacagg
    10861 tactgtcatt attggtattc aacttaaaga tcatgatgat ttaatacaac tcaaacaacg
    10921 tgtaaatcat ttcgatcctt ccaatattta tattaatgaa aataagatgt tatattcatt
    10981 gttaatttaa cacatagtaa gaaaaacagt cataaattga tttctaattg aaatcatctt
    11041 atgactgctt tttattatac tttacatttc tcgtttcgtc agattcaaac gttttcactt
    11101 cgccaagcca tctttctttg tgtttgcttt tattttgacg ttttagacat aaaaaaagag
    11161 accttgcggt ctcaatgcgg ctcatcgcat ccactttttg cctggcaacg ttctactcta
    11221 gcggaacgta agttcgacta ccatcgacgc taaggagctt aacttctgtg ttcggcatgg
    11281 gaacaggtgt gacctccttg ctatagtcac cagacatatg aatgtaa
//
LOCUS       DALAGD010000093        11216 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.094, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000093 DALAGD010000000
VERSION     DALAGD010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11216
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.094"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            207..1664
                     /locus_tag="O2701_002181"
     CDS             207..1664
                     /locus_tag="O2701_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500488.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCZ4294059.1"
                     /translation="MKCLIRFILVLGLLISSAMVYINPTAHAEQDQTWEKIKERGELR
                     VGLSADYAPMEFEHTVNGKTEYAGVDIDLAKKIAKDNNLKLKIVNMSFDSLLGALKTG
                     KIDIIISGMTSTPERKKQVDFSDSYMMTKNIMLVKKDKVNEYKDIKDFNNKKVGAQKG
                     TEQEKIAQTEIENASITSLSRLPDVILALKSGKVEGAVVEKPVAEAYLKQNPKLGISN
                     VKFNEEEKDTVIAVPKDSPKLLSQINKTIKEVKDKGLIDKYMTNAANAMNDDSGFISK
                     YGSFFLKGIKITILISLIGVALGSILGAFVALMKLSKIKIISWIASIYIEILRGTPML
                     VQVFIVFFGITAALGLDISALVCGTIALVINSSAYIAEIIRAGINAVDKGQMEAARSL
                     GLNYRQTMKSVIMPQAIKNILPALGNEFVTLIKESSIVSTIGVGEIMFNAQVVQGISF
                     DPFTPLIVAAALYFVLTFVLTRIMNMIEGRLNASD"
     gene            1657..2379
                     /locus_tag="O2701_002182"
     CDS             1657..2379
                     /locus_tag="O2701_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCZ4294060.1"
                     /translation="MIKINNLNKVFGDNEVLKDINLEINQGEVVAIIGPSGSGKSTLL
                     RCMNLLEVPTKGQVIFEGNDLTEKGTQVDKLRQKMGMVFQNFNLFPHKKVVDNIILAP
                     KLLKKDNNDELHKEALSLLDKVGLKEKADVYPNQLSGGQKQRVAIARALAMHPDVILF
                     DEPTSALDPEVVGDVLKVMKDLAKEGMTMVVVTHEMGFAKDVSDKVIFMADGVVVESG
                     TPVEIFEQPQHERTQNFLARVL"
     gene            2530..3657
                     /gene="queG"
                     /locus_tag="O2701_002183"
     CDS             2530..3657
                     /gene="queG"
                     /locus_tag="O2701_002183"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCZ4294061.1"
                     /translation="MDTKQLKQDIIDYAYTIGIDSIGFTTADPFDELKQKLEAYHANG
                     YASGFEESDIALRTEPKLSLPTARSIIAIAVGYPNKLKGAPKSVRGDRRGLFARASWG
                     QDYHTIMRKRLDMLAAFIESKVPDVEIKSMVDTGVLSDRAVAERAGLGFVGRNGFVIN
                     PKLGTWTYLGEMLVSIPFEPDDPLLDSCGDCTICVDRCPTSALVGNGQLNSQKCISFL
                     TQTKGYMPDQYRYKIGNRLYGCDTCQQVCPKNRGINTEQDDIILEPEILKPRLVPLLR
                     MSNKEFKQTYGHLAGAWRGKKPIQRNAILALAHFNEVDAIPELKKVATTDERPLIRAT
                     AYWAIGQILGEEARDFINANYDQEDAEVQNEMIKGLDTRRE"
     gene            3662..4132
                     /gene="trmL"
                     /locus_tag="O2701_002184"
     CDS             3662..4132
                     /gene="trmL"
                     /locus_tag="O2701_002184"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCZ4294062.1"
                     /translation="MTNHIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKM
                     LKRAGLDYWEFVNITYHDSIEAFFEVTNGEYYLLTKFGKKTYSDFDFSNHDKDFYFIF
                     GKETTGLPDWVKEKYQDTALRIPMSEHIRSLNLSNTAALLIYEALRQQDFPGLN"
     gene            4291..4890
                     /locus_tag="O2701_002185"
     CDS             4291..4890
                     /locus_tag="O2701_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HCZ4294063.1"
                     /translation="MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAP
                     VLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGVPAGQVYPIAYEKDAIELIADKI
                     KTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
                     TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA"
     gene            4915..5067
                     /locus_tag="O2701_002186"
     CDS             4915..5067
                     /locus_tag="O2701_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294064.1"
                     /translation="MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKN
                     SEEDSQ"
     gene            5844..6239
                     /locus_tag="O2701_002187"
     CDS             5844..6239
                     /locus_tag="O2701_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294065.1"
                     /translation="MLGKYWIQLLIATVIISLISIKAFPLAIGALYLPVIFKVIKLQL
                     NLSKGLIDDVNAQTFIKSNQSGIVISVICCLLITAILYYTLDGFYASLTGVLGTLVAL
                     NPYTTIVSAVLYILTAIATVEATKTKYRN"
     gene            6435..7820
                     /gene="fumC"
                     /locus_tag="O2701_002188"
     CDS             6435..7820
                     /gene="fumC"
                     /locus_tag="O2701_002188"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCZ4294066.1"
                     /translation="MSVRIEHDTFGEIEVPADKYWGAQTERSKRNFPVGKERMPIEVV
                     YGFAQLKRAAAIANFDLGKLSEAKKDAIVYACDQILSGELDEHFPLVVWQTGSGTQSN
                     MNVNEVVSYVANMYLKDHQSDESIHPNDDVNKSQSSNDTFPTAMHVALYQEVETKLEP
                     ALKLLRNTLKEKEDKFDSIIKIGRTHLQDATPIKLGQEISGWRYMLDRCETMLSESKK
                     HILNLAIGGTAVGTGINAHPEFGDKVAHYISENTGYPFVSSENKFHALTAHDEVVQLH
                     GTLKALAGDLMKIANDVRWLASGPRAGLAEISIPENEPGSSIMPGKVNPTQCEMLTMV
                     AVQVMGNDTVVGFASSQGNFELNVYKPVIMHNTLQSIYLLADGMETFNNNCAVGIEPI
                     EENIDNYLNQSLMLVTALNPHIGYEKAAQIAKKAHKEGLTLKESAIQTGYVTEEQFEA
                     WIKPEDMVDPH"
     gene            complement(8283..9104)
                     /locus_tag="O2701_002189"
     CDS             complement(8283..9104)
                     /locus_tag="O2701_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCZ4294067.1"
                     /translation="MKFKIPENFNDLSLRDIFQQLKVPKKDLHHLNMSKDITINDKPA
                     RLMDKVHTGDDVFVPTIDEKSNYVPSYRYAQIKYEDDDMAIVMKPKGVKTHPNDLKES
                     NTLMNHVIYTIDSDYVEPIHRLDQETVGLLIVAKNPLMKKILDRMLEDNDITRIYKAN
                     VKALLPLKPQTIDMPIGKDKFHSNKRRVSPTGQRAITHILTSKMIKEAVCQLEIKLDT
                     GRTHQIRVHLAEIGHPVIGDPLYGDSTLRQLELESYKIEFVHPLTKEVISVSLDD"
     gene            9267..10379
                     /locus_tag="O2701_002190"
     CDS             9267..10379
                     /locus_tag="O2701_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HCZ4294068.1"
                     /translation="MEQRTRLALLKEIAEFLNEETEMYSMTQGALKYLIEGSNFTTGW
                     IFFINSVGEHELVSHVALPQSLTADHCHYIKDGSCWCVKAFNQRRLMKASNIVNCSRI
                     NLASKAFPSQNDNITHHATVPLKSGQEQFGILNVASPNTEIYSDEDLELLESVAFQLG
                     SAIKRIYLTDREKEAAKINERNRLARDLHDSVNQMLFSVKLTAHAAYGMSNESIAKQA
                     FKTIEETSQNAVNEMRALIWQLKPVGLEQGLIHALTAYSKLMHIQLNVNVEGLIDLSN
                     EIEENIYRALQECINNVKKHADTNKMDLTLKQMNDILYIDVIDYGQGFEIDNVQIASS
                     HGINNIKQRVKLLRGKVTFHSQPTKGTQIQFTIPIK"
     gene            10401..11024
                     /locus_tag="O2701_002191"
     CDS             10401..11024
                     /locus_tag="O2701_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCZ4294069.1"
                     /translation="MNKVILVDDHYIVRQGLRFLLSTIENIEVLQDFADGETFLEYLK
                     EHEHPDIVLLDLVMPGMNGIEITEYIKAHYPDIKVLVLTSYVDDEHVISAINKGADGY
                     EMKDVEPQQLIETIRRVMNGEKMIHPKAQDVFETVSQKPHYTNKLSKREIEVLREMVK
                     GKTNKEIAETLFVSEKTIKTHVSHIFSKLQVSDRTQAAIYAMENKLI"
BASE COUNT         4062 a         1553 c         2044 g         3557 t
ORIGIN      
        1 tactgtcaat aagatattga tagtagcgct tttttgattg attttaaaat gtgagatttg
       61 ttcacgtata atagcggatt ttttatgtac agtcttttaa aaagaagctt ttctttatat
      121 tgataaaaat acatttataa aacactattg catcaaaatg tattaatatg tataattatg
      181 aatattaatt agggagggct gtataaatga agtgtttaat taggtttata ttagtattag
      241 ggttgttaat tagcagtgct atggtgtata tcaatcccac agcacatgct gaacaagatc
      301 aaacatggga gaagattaaa gaacgcggtg aacttagagt gggtctttct gcagattatg
      361 caccaatgga atttgagcat acagttaatg gtaagactga gtatgcaggt gtagatattg
      421 atttagctaa aaaaattgcg aaagataata atttaaaatt aaaaatcgtc aatatgtcat
      481 ttgatagttt gttaggagct cttaaaactg gaaaaattga tattattatt tccggaatga
      541 cttcaacgcc tgaacgtaag aagcaagttg atttttcaga ttcatatatg atgactaaaa
      601 atatcatgct tgtaaagaaa gataaagtta atgaatataa agatatcaaa gactttaata
      661 ataaaaaagt aggggcacaa aagggaactg aacaagaaaa aatcgctcaa accgaaattg
      721 aaaatgcatc tattacttca ttaagccgtt taccagacgt tatattagca ttgaaaagtg
      781 gaaaggttga aggtgctgta gttgaaaaac ctgtagcaga agcatattta aaacaaaatc
      841 ctaaattagg aatttccaat gtgaaattta atgaagaaga aaaagataca gtgatagcag
      901 tgccaaaaga ttcaccaaaa ttattgtcac aaattaataa aacgattaag gaggttaaag
      961 ataaaggatt aatcgataaa tatatgacta atgctgcaaa tgcgatgaat gatgacagtg
     1021 gttttatttc taagtatggt agttttttct tgaaaggaat taagattaca atattaattt
     1081 cacttatcgg tgttgcatta ggttctattt taggtgcatt cgttgcgtta atgaaattaa
     1141 gtaaaataaa aattatttca tggattgctt ctatctatat cgaaatatta agaggaacac
     1201 caatgttagt tcaagtattt atcgtattct ttggtataac tgctgcatta ggactagaca
     1261 tttcagctct tgtttgtgga acaattgcat tagttattaa ttcctcagct tacattgctg
     1321 aaattattcg tgcaggtata aatgctgttg ataaaggcca aatggaagcc gcacgtagtc
     1381 taggtttgaa ttatagacaa acgatgaaaa gtgtaattat gccacaagca attaaaaata
     1441 ttttaccagc tttaggtaat gaatttgtca ctttaattaa agaatcatct attgtgtcaa
     1501 caattggagt tggcgaaatt atgtttaatg cacaagtggt tcaaggtatt tcatttgacc
     1561 cattcacacc attaatagtg gcagcagcat tatactttgt cttaacattt gtacttacac
     1621 gcattatgaa catgattgaa gggagattga atgccagtga ttaaaataaa caatcttaat
     1681 aaagtttttg gagataatga agttttaaaa gatatcaatc ttgaaatcaa tcaaggggaa
     1741 gtagtagcaa taataggtcc atctggtagt ggtaaaagta cattgttaag atgtatgaat
     1801 ttattagaag tacctactaa aggtcaagtg atttttgaag gcaatgactt aacggaaaaa
     1861 gggacacaag tagataaact acgtcaaaaa atgggtatgg tatttcaaaa cttcaaccta
     1921 tttccacata aaaaagttgt cgataatatt attttagctc ctaaattatt aaagaaagat
     1981 aataacgatg aattacataa ggaagcattg tcgttattag ataaagtggg attaaaagaa
     2041 aaagcagatg tatatccgaa tcaattatca ggtggtcaaa aacaaagggt agcaattgca
     2101 agagctttag caatgcatcc agatgttatt ttattcgatg aaccaacttc agcattagat
     2161 cctgaggtag ttggtgatgt attaaaagta atgaaagacc tagccaaaga aggtatgacc
     2221 atggtggttg tgacacatga aatgggattt gccaaagatg taagtgacaa agtcatattt
     2281 atggcagatg gcgttgtcgt agagtcaggc acaccagtcg aaatatttga acaaccgcaa
     2341 catgaaagaa cacaaaattt cttagcaaga gtattataac aacctaacga agcttgaata
     2401 tatgatacgc accacaaagt tatatcatat attcaagctt tttattatta aaattatgtg
     2461 aaatgaaacc tcaatatgaa taatggttgt gttaaaataa catagtatta ttttatgggg
     2521 ggcatacgat tggatacaaa gcaattaaag caagatatca ttgactatgc atatacaatt
     2581 ggtatcgaca gtattggatt tactactgcc gatccctttg atgaattgaa gcaaaagcta
     2641 gaagcatatc atgcaaatgg ttatgcctca ggatttgaag aatctgatat tgctttacga
     2701 acggagccta aattatcctt accaacagca aggtcaatca tagcaattgc agttggttat
     2761 cctaacaaac tgaaaggtgc acctaagagt gttagaggtg atcgcagagg cttatttgct
     2821 agagcatcgt ggggtcaaga ttatcataca attatgcgta aacgattaga catgttagct
     2881 gcatttattg aatctaaagt tccagatgtt gaaatcaaat ctatggtaga tacgggtgta
     2941 ttatcagata gggcagtagc agaacgtgca ggtttaggat ttgttggtcg taatggcttt
     3001 gtcatcaatc ctaaactagg aacatggaca tacctcggtg aaatgttagt cagtatacct
     3061 tttgaacctg atgatccatt attagatagc tgtggtgatt gtacaatttg tgttgatcgt
     3121 tgtccaacaa gtgcattagt tggaaatggc caattaaata gtcagaaatg cattagtttt
     3181 ttaacgcaaa caaaaggcta tatgcctgac caatatcgtt ataaaattgg gaatagatta
     3241 tatggttgcg atacgtgtca acaagtttgt ccgaaaaata gaggtattaa taccgaacaa
     3301 gatgacatca ttttggaacc agaaatttta aagccaagat tagtaccttt actacgtatg
     3361 tctaataaag aattcaaaca aacatatggt caccttgcag gtgcttggcg tggtaaaaag
     3421 ccgatacaac gaaatgctat tttagcattg gcgcatttta atgaagtgga tgcaattcca
     3481 gaattgaaaa aagttgcaac aacagatgaa agaccgttga ttcgagcaac tgcttattgg
     3541 gccattggtc aaattcttgg ggaagaagca agagatttta tcaatgctaa ttatgatcaa
     3601 gaggatgcag aagttcagaa tgaaatgata aaaggattag acacaaggag agaatagtaa
     3661 tatgacaaat catatcgttt tatatcaacc agaaatccca gctaatactg gtaacattgc
     3721 acgtacgtgt gcaggtacaa acacacattt acatttaatt aaacctcttg gatttagaac
     3781 tgatgataaa atgttaaaaa gagccggttt agattattgg gaatttgtga atattacgta
     3841 tcatgatagt attgaagcat tttttgaagt cacaaatggc gagtattact tattaacaaa
     3901 atttggtaaa aagacttata gtgattttga tttttcaaat catgacaaag atttttactt
     3961 catttttgga aaagaaacga caggattacc agactgggtg aaagagaaat atcaagacac
     4021 agcgttaaga attcctatga gtgaacatat tcgttcatta aatttatcaa atactgctgc
     4081 gttgttaatt tatgaagcgt tacgtcaaca agattttccg ggattgaatt aaaatttact
     4141 cattttcatt ataaaaatga tgcttaagtg ctatatttag agtataagca tgttaaagtt
     4201 aaatggtttg atatattgaa agttagggta taataattat taatatcgcc gtttttatgt
     4261 aaaatgcaaa ctaagaggag tgtagaaatc atggcaatga actttaaagt ctttgacaat
     4321 agtcaacttg tagcagaata tgctgctgat attattagaa agcaatttaa caataatcct
     4381 actacaattg caggttttca tttagataca gatcaagcgc cagttctaga tgaattaaag
     4441 aaaaatgttg aaaaacatgc tgttgatttt agccaaataa atattttaga ttatgacgat
     4501 aaaaaatcat atttcgaagc gttaggtgta ccagcaggtc aagtttatcc aattgcttat
     4561 gaaaaagatg caatcgaatt aatcgctgat aagattaaaa ctaaagaaaa taaagggaaa
     4621 ttaacattac aagttgtttc tatcgatgag caaggtaagt taaatgttag tattcgtcaa
     4681 ggactaatgg aagcaagaga aattttctta gtagtgacag gtgctaataa acgagatgta
     4741 gttgaaaaat tatatcaaga aaatggtaaa acaagcttcg aaccagccga tttaaaagca
     4801 catagaatgg taaatgttat tcttgataaa gaagcggctg caggtttacc tgaagatgtt
     4861 aaagcttact ttacgtcacg ctttgcttaa ttaaaaatct gtgaggtatt gattatgtct
     4921 aaagacaaag atccaaaatt aaattatcat gaagaagaaa acagtatggt aacggatttt
     4981 gaagatttaa aagaattagg taaagaaatg gaacaaatct ctgatcaaaa tgatcaagaa
     5041 aagaattctg aagaagacag tcagtaaagt attataattg tgtaatattc agttatttat
     5101 catgtaaata atgtatagta tagaacatat atcaaatagg attgattatc ttgaatatga
     5161 agttaggtag cattttgtta cctagctttt ttattatatt catagaagct gactttctgc
     5221 tagtttcagt gttggggctg tttccccaac ttacattgtt tgtagaaatt gaggatccaa
     5281 tttttctatg ttgtggcccc gccaacttgc atgttatttt aagctgactt tccaccaact
     5341 tctgagtttg tggcccgttt ccctaatttg caatgtctgt agaaattaga aatccgatct
     5401 cactattttg gggcctcgcc aacttgcatg ttattttaag ctgactttcc accagtttct
     5461 gagtttgtgg cccgtttccc caatttgcaa tgtctgtaga aattgggaat ccaattccac
     5521 tatgttggag ctccaccaac ttgcatgaaa tagaagctga cattccgaag cctctatgtt
     5581 gtggactgca gtcaactact gccaatataa ctttgtaaag cataaaacat tgatttgtgt
     5641 accagacata gtaatatatc aaaagttcaa ttttaaatat taaaacacca tttaaacgga
     5701 attgataatt aattactcca gacataattg aaagaagtca tctatcagta atatttttaa
     5761 tgaaatcatt aaaccgcgtt tcttaatatg ccaagattat gatatgctat tgaagaatga
     5821 aattagagga gatttatata tttatgttag gaaaatactg gattcaatta ttgatagcaa
     5881 cagttattat aagtttaata tcaattaaag cttttccatt ggcaattggg gcattatatt
     5941 tacctgttat tttcaaagta ataaaattac aactcaattt atcaaaagga ttaattgatg
     6001 atgttaatgc gcaaacattc attaaaagta atcaatcagg tattgtaatc agtgtaattt
     6061 gttgtttatt aataacagca atattgtatt atacacttga tgggttttat gcgagtttaa
     6121 caggagtttt aggtacgctt gttgctctaa atccatatac gacaattgtt agtgctgtat
     6181 tatatatact tacagctatt gctacggttg aagctactaa aacaaagtat cgtaattaaa
     6241 cttagttcaa taataactaa ataatgatag ataacacact aatcgtatga ccgtatcact
     6301 gtaataaagg gtgaacacgt gcgattagtg tatttttatt tcaaattatg aaaacgtttt
     6361 aatcataaat tgctcaatta aattatttaa tgatatacta cagttaaata aacagtgata
     6421 aggggagaaa ttgaatgtca gtaagaattg aacatgatac ttttggagaa atagaagtac
     6481 ctgcagataa atattggggt gctcaaacag aaagaagtaa acgtaatttc ccagttggta
     6541 aagagcgtat gccaatcgaa gtagtttatg gttttgcaca actaaagcgt gcagcagcaa
     6601 tagctaattt tgatttagga aaattaagcg aggcaaagaa agatgccatt gtatacgcat
     6661 gtgatcaaat tttatcaggt gaattagatg aacacttccc actagttgta tggcaaacag
     6721 gaagcggtac acaaagtaat atgaatgtga acgaagtagt aagttatgtt gctaatatgt
     6781 atttaaaaga tcatcaaagt gatgaaagta tccacccaaa tgatgatgta aataaatctc
     6841 aaagttcgaa tgatacattc ccaactgcta tgcacgttgc attatatcaa gaggttgaaa
     6901 caaaattaga acctgcatta aaacttttaa gaaatacttt gaaagaaaaa gaagataaat
     6961 ttgattcaat tattaaaatt ggtcgtacac atttacaaga tgcaacgccg atcaaactag
     7021 gacaagagat tagtggctgg cgttatatgc ttgaccgttg tgaaacaatg ttatctgaat
     7081 ctaagaagca cattttaaat cttgccatcg gtggtacggc tgttggtact ggtattaatg
     7141 cgcatcctga atttggtgat aaagtggcac attatatttc agaaaatacg ggttatccat
     7201 ttgtatcttc tgaaaataaa ttccacgcac ttacagcgca tgatgaagtt gttcaattgc
     7261 atggaacatt gaaggcatta gcaggagact taatgaaaat tgctaatgat gtgagatggt
     7321 tggcttcagg gccacgagct ggtttggcag aaatttctat ccctgaaaat gaaccaggtt
     7381 catcaattat gcctggtaaa gttaatccta cacaatgtga aatgttaaca atggttgcag
     7441 tccaagtaat gggtaatgat acagttgttg gcttcgcaag ttcacaaggt aactttgaat
     7501 tgaatgttta taaaccagtt attatgcata atacactaca atcaatttat cttttagctg
     7561 atggtatgga aacatttaat aacaattgtg cagtgggcat tgaaccaatc gaagagaata
     7621 ttgataatta tttaaatcaa tcattaatgt tagttactgc attaaatcca catattggtt
     7681 atgaaaaagc agctcaaatt gctaagaaag cccataaaga aggtttaact ttaaaagaat
     7741 ctgcaattca aactggatat gttacagaag aacaatttga agcatggatt aaaccagaag
     7801 atatggtaga tcctcattaa ttaaatatta atataaaata aagtttgggc attacaggta
     7861 gatgtagtgt tcaagcttta ttttttgtct atgtcaaatt gaacttataa gtctgatttg
     7921 atatagagcc atgaaaagga aaaacttttc atggagtccg ttttattata gaacctattt
     7981 tttatatgat gaattgttgt acatgtattg gccaaacaac ctgtgataaa aatcttatga
     8041 tgtcatgtat tgtccataga tacacgtaac gcagttgaaa aagactacaa gtcattatcc
     8101 aaggatgtta taaaatgaat aattactaaa atatcaaaat ataaaatcct aagcatcgta
     8161 aaaaacaaaa aaaccgaaca tctgaaaaaa ttaataattt tccatctatc cgatttaagt
     8221 tgatacttga tttagttaca atgcggtatt gcatgtttaa atatcatgca aaactaatca
     8281 aattagtcat ccaaagaaac ggaaatgact tccttagtca agggatgcac aaactctatt
     8341 ttgtaacttt caagttctaa ttgtcttaac gttgaatcac catataaagg atcaccaata
     8401 acagggtgac caatttcagc taaatgcaca cgaatttgat gagtacgtcc agtatccaac
     8461 ttgatttcaa gttggcacac agcttctttt atcatttttg aagttaaaat gtgtgtaatt
     8521 gcacgctgtc cagtaggaga cacacgtcgt ttattcgaat ggaatttatc tttaccaatt
     8581 ggcatatcaa tcgtttgtgg ttttaaaggt aaaagtgcct taacatttgc tttgtatatc
     8641 cgcgtaatat cattgtcttc taacatgcga tcaagaattt ttttcattaa aggattttta
     8701 gcaacaatta ataatcctac tgtttcctgg tccagtcgat gaattggttc gacatagtca
     8761 ctatcaatag tgtaaatcac atgattcatt aaagtattgc tttcttttaa atcattaggg
     8821 tgagtcttaa cacctttagg tttcattacg attgccatat catcgtcttc gtatttaatt
     8881 tgtgcataac gataacttgg aacataatta cttttttcat cgatggttgg aacaaataca
     8941 tcgtcgccag tatgcacttt atccattaat cgcgcaggtt tatcattaat agtaatatct
     9001 ttagacatat ttaaatgatg taaatctttt ttaggtacct taagttgttg gaaaatatct
     9061 cgtaaactta agtcattaaa gttttctggt attttaaatt tcataaaatc ctcctatagt
     9121 ctttaatatc atatcacaaa tcagttctcc gaaatataac tattgagtaa tcaaggccat
     9181 tttatcatgt ttacgtctaa gatttaaaaa tataataact caatgtataa tagttaatac
     9241 acgtaaaaat gaattggagc gatttgatgg aacaaaggac gcgactagct ttattaaaag
     9301 agattgccga atttttaaac gaagaaactg aaatgtatag catgacccaa ggtgcgttaa
     9361 aatatttaat tgagggcagt aatttcacga caggctggat atttttcatc aatagcgtag
     9421 gtgaacatga acttgtatca catgtggctc taccacaatc tttgacagca gaccactgtc
     9481 actatattaa agatgggtct tgttggtgtg tgaaagcatt taatcaacgt aggctaatga
     9541 aagcgtcgaa tatcgtcaat tgttctcgta ttaacttagc ctctaaagca tttcctagcc
     9601 aaaatgacaa tattacacat catgcaacgg taccacttaa atcgggtcaa gaacaattcg
     9661 gcattttaaa tgtagcgtct ccaaatactg aaatatatag cgatgaagat ttagagttgc
     9721 tagagtctgt cgcatttcaa ttagggtcag ccatcaaacg tatttattta acagaccgtg
     9781 aaaaagaagc ggcaaaaatt aatgaacgta atcgtttagc tagagattta catgattcag
     9841 taaatcaaat gttattttct gtaaaactaa cagcacatgc tgcgtatggc atgtcaaacg
     9901 aatccatcgc taaacaagca ttcaagacga ttgaggaaac gagtcaaaat gccgttaatg
     9961 aaatgcgtgc attgatttgg caacttaagc cagttggact tgagcaaggt ttaattcatg
    10021 ctttgacagc atatagtaaa ttaatgcata tacaattaaa tgtaaatgta gaaggtttaa
    10081 tcgatttatc taatgaaatc gaagaaaaca tatacagagc attacaagag tgtattaata
    10141 atgttaagaa acatgctgat acgaataaaa tggatcttac attaaaacaa atgaacgata
    10201 tactatatat tgacgtgatt gattatggtc aaggatttga gatcgataat gttcagatag
    10261 cttcatcaca cggtattaat aacataaaac aaagagttaa attattaaga ggtaaagtga
    10321 cctttcattc tcagccaaca aagggtacac aaatacaatt cacaattcct ataaaatagc
    10381 ttgatttaag gagataaccc atgaacaaag taatattagt agatgaccat tatattgtgc
    10441 gacaaggatt gcgattttta ttatccacga ttgaaaacat agaagtttta caagactttg
    10501 cagatggaga aacattttta gaatatttaa aagagcatga gcaccctgat attgtgctat
    10561 tagatttagt gatgcctggc atgaatggta ttgaaattac ggaatatatt aaggcacatt
    10621 atccggatat taaagttttg gtattaacaa gttatgttga tgatgaacat gtaatttcag
    10681 caatcaataa aggtgctgat ggttatgaaa tgaaagacgt tgagcctcag caattaattg
    10741 aaactattag acgagttatg aacggtgaaa aaatgataca tcctaaggca caagatgtat
    10801 tcgaaacagt tagccaaaaa ccacactaca cgaataagtt gtcaaagaga gaaattgaag
    10861 tgttacgtga aatggttaaa ggtaaaacaa ataaagagat tgcagaaact ttatttgtat
    10921 ctgaaaaaac aattaaaaca catgtcagtc atatatttag taaattacaa gttagcgatc
    10981 gtacacaagc agcaatttat gcaatggaaa ataagttgat ttagaatgag cttttaaata
    11041 tttgtcgaaa aatagacaat ttttaatctg attttgatga attttttata gcgatagggt
    11101 ttattataca gttttctttg taaatttgat atcattataa aagtgtaagt tttatcaaat
    11161 ctagtgatta tgcataaaaa tttacttcat ttactttcgc ttcattataa agtaaa
//
LOCUS       DALAGD010000094        10912 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.095, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000094 DALAGD010000000
VERSION     DALAGD010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10912
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.095"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(254..946)
                     /locus_tag="O2701_002192"
     CDS             complement(254..946)
                     /locus_tag="O2701_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCZ4294070.1"
                     /translation="MNYSPKQGVRSHGLPHDPFKSSTVPRPIGWISTVSKDGKDNLAP
                     YSQYQNLTWDPPMVMFAANQSVLGDHERKDTVKNAEETGWFVWNMATYDLREAVNLSS
                     KALPPEEDEFNFADVTKEKCIEAPGYRVKESPVHFECEYVQTIRIPTGDPVSTVDIVI
                     GRVAQVHIDDKVILDNGKLDIKSIKPIARLGYYDYTVVNEIFEMKAPAASKEELAGLE
                     GRNFDNQSDEKI"
     gene            1182..1640
                     /locus_tag="O2701_002193"
                     /pseudo
     CDS             1182..1640
                     /locus_tag="O2701_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226788.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1627..1929
                     /locus_tag="O2701_002194"
     CDS             1627..1929
                     /locus_tag="O2701_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294071.1"
                     /translation="MRTNDHITDLDAIELSSGWNYNSKLETGKNALLSDSLLRDIKVL
                     SSNAKGDADILETELRAVINSMSQNEGKSVDALKNSTASLKNNLTKLKEQLDKTTH"
     gene            2083..2442
                     /locus_tag="O2701_002195"
     CDS             2083..2442
                     /locus_tag="O2701_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294072.1"
                     /translation="MILRYATNLKLAKELYQASKPKLQEDKGMIELFEHTFGLQKELV
                     KYVGIAEATTAALYSASFINKNISRLASLSTIGILSVAAYKHFEAGQGKKGAQHALNL
                     LGLATLSLLDTFSCNKK"
     gene            complement(2527..3138)
                     /locus_tag="O2701_002196"
     CDS             complement(2527..3138)
                     /locus_tag="O2701_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCZ4294073.1"
                     /translation="MYTFDAQSLTARENYKLLIGSIIPRPIAFVTTLNQDASVNAAPF
                     SFFNIVNNHPPMIAIAVQRAGGKRKDTALNIERTGDFVVHITDEDNVQDINETAAPLA
                     YGDSELSRTELSLLTSTTIKTPGIKDAKMRFECKLSQMILLGDVLDGADLIIGEIVTY
                     HIDDSIYEGDFKINPHALQAVSRLAGNDYAKLGGIFTIKRPEK"
     gene            complement(3217..3693)
                     /locus_tag="O2701_002197"
     CDS             complement(3217..3693)
                     /locus_tag="O2701_002197"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HCZ4294074.1"
                     /translation="MAVVYVAGGCLWGVEAFFATIPGIIHTEAGRANGRSSKLDGPYD
                     GYAECVKLHFDDRMLTITDIMNYLFEIIDPYSVNQQGNDIGQKYRTGLYSCVDDHLIE
                     ARQFIERRKDRDQIAVEVLPLSNYIKSAEEHQQHLEKYPEDMHMCHISKDLLNKYK"
     gene            complement(3696..4196)
                     /locus_tag="O2701_002198"
     CDS             complement(3696..4196)
                     /locus_tag="O2701_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4294075.1"
                     /translation="MVLRKVIMQDLDQIIALENIGFSPEEAATREALKLRIEQIQETF
                     IVAEKNDEVIGYINGPVIKERYISDDLFKNVSTNNSEGGYISVLGLVVAPNYQGQGIA
                     GRLLNYFETLAKNHHRHGVTLTCRESLISFYEKYGYRNEGVSESCHGGIKWYNLVKNI
                     KKDDEV"
     gene            complement(4431..5198)
                     /locus_tag="O2701_002199"
     CDS             complement(4431..5198)
                     /locus_tag="O2701_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HCZ4294076.1"
                     /translation="MIDIHNHILVDIDDGPKTIEKSIALLKQAKDEGVTSIVATPHHL
                     HPRYDNTFQQVLVKLAELRTHPEVQALDIKLFPGQEIRITDSILKGLDNGSIQGINRS
                     KYLLIEFPTGEVPHYTKQLFFEIQSRGYIPIIAHPERNRSIAKNPEILYELVANGALS
                     QLTSSSLVGGFGKNIQKLSLQFIECNLAHFVASDAHSCDQRPFLMQELFHNHKLKKYS
                     NDIEALLRNASSVINDNFVYLDRPTKPGKVKSFLKWF"
     gene            complement(5195..5887)
                     /locus_tag="O2701_002200"
     CDS             complement(5195..5887)
                     /locus_tag="O2701_002200"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HCZ4294077.1"
                     /translation="MTNTRRSTSSLIVHEQPKSPISEKFRGIRSNIMFANPDSAVQSI
                     VITSEAPGAGKSTIAANLAVAYAQAGYKTLIVDGDMRKPTQHYIFNLPNNEGLSSLLL
                     NWSTYQDSIISTEIQDLDVLTSGPIPPNPSELITSRAFANLYDTLLMNYNFVIIDTPP
                     VNTVTDAQLFSKFTGNVVYVVNSENNNRDEVKKGKELIEATGAKLLGVVLNRMPKDKS
                     ASYYAYYGTDES"
     gene            complement(5904..6566)
                     /locus_tag="O2701_002201"
     CDS             complement(5904..6566)
                     /locus_tag="O2701_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapA"
                     /protein_id="HCZ4294078.1"
                     /translation="MKEKFDLVKLLNILKKNIKLLLILPAICLVVSAALTFFVMPDKY
                     TASTQILVNMKKSSSDLAFQNVQSSLQSVNTYTEIIKSPRILDKVSREFDGQYSTAEL
                     NSFLKVTNQTNSQIITVSVTTGNKSESDKIVNKISKVFAHDMPKIMSVDNVTILSSAH
                     DNAVKVSPIVSVNLVISIIVGIVLAILIIFLKELLDKRIKTEEDVESQLGLPILGSIQ
                     KF"
     gene            complement(7414..7974)
                     /gene="icaR"
                     /locus_tag="O2701_002202"
     CDS             complement(7414..7974)
                     /gene="icaR"
                     /locus_tag="O2701_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ica operon transcriptional regulator IcaR"
                     /protein_id="HCZ4294079.1"
                     /translation="MKDKIIDNAITLFSEKGYDGTTLDDIAKSVNIKKASLYYHFDSK
                     KSIYEQSVKCCFDYLNNIIMMNQNKSNYSIDALYQFLFEFIFDIEERYIRMYVQLSNT
                     PEEFSGNIYGQIQDLNQSLSKEIAKFYDESKIKMTKEDFQNLILLFLESWYLKASFSQ
                     KFGAVEESKSQFKDEVYSLLNIFLKK"
     gene            8138..9376
                     /gene="pgaC"
                     /locus_tag="O2701_002203"
     CDS             8138..9376
                     /gene="pgaC"
                     /locus_tag="O2701_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HCZ4294080.1"
                     /translation="MQFFNFLLFYPVFMSIYWIVGSIYFYFTREIRYSLNKKPDINVD
                     ELEGITFLLACYNESETIEDTLSNVLALKYEKKEIIIINDGSSDNTAELIYKIKENND
                     FIFVDLQENRGKANALNQGIKQASYDYVMCLDADTIVDQDAPYYMIENFKHDPKLGAV
                     TGNPRIRNKSSILGKIQTIEYASLIGCIKRSQTLAGAVNTISGVFTLFKKSAVVDVGY
                     WDTDMITEDIAVSWKLHLRGYRIKYEPLAMCWMLVPETLGGLWKQRVRWAQGGHEVLL
                     RDFFSTMKTKRFPLYILMFEQIISILWVYIVLLYLGYLFITANFLDYTFMTYSFSIFL
                     LSSFTMTFINVIQFTVALFIDSRYEKKNMAGLIFVSWYPTVYWIINAAVVLVAFPKAL
                     KRKKGGYATWSSPDRGNTQR"
     gene            9340..9645
                     /gene="icaD"
                     /locus_tag="O2701_002204"
     CDS             9340..9645
                     /gene="icaD"
                     /locus_tag="O2701_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular adhesion protein D"
                     /protein_id="HCZ4294081.1"
                     /translation="MVKPRQREYPTLKSSLNIVRETALIAISCVFWIYCLVVLLVYIG
                     TIFEIHDESINTIRVALNIENTEILDIFETMGIFAIIIFVFFTISILIQKWQRGRES"
     gene            9642..10514
                     /gene="icaB"
                     /locus_tag="O2701_002205"
     CDS             9642..10514
                     /gene="icaB"
                     /locus_tag="O2701_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intercellular adhesin biosynthesis
                     polysaccharide N-deacetylase"
                     /protein_id="HCZ4294082.1"
                     /translation="MKYRKFIILVLSILIILPVSTLDGHHIANADDDSPKKLKYKENS
                     ALALNYHRVRKANFLNNFIYFFSSSKEIKNYSVSQSQFESQIKWLKSHDAKFLTLKEF
                     LYYKKKGKFPKRSVWINFDDMDETIYENAYPILKKYKIPATGFIITGHVGEENFHNLD
                     MISKKELKEMYKTGLWEFETHTHDLHNLSKNNKSKLMKASEATIIKDLNKSEKYLTKN
                     FKKSQKTIAYPYGLMNDDKLPVIKKAGLKYGFSLEEKAVTPNSNDYYIPRILISDDAF
                     EHLIKRWDGFHEKD"
     gene            10501..>10912
                     /locus_tag="O2701_002206"
     CDS             10501..>10912
                     /locus_tag="O2701_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCZ4294083.1"
                     /translation="MKKIRLELVYLRAIICAIIIITHLLTQITLKHENMEGGSLVLQF
                     YIRNIVIFGTPCFIILSQLLTTLNYQKVTYRYLTTRVKYILIPYILMGLFYSYSESLL
                     TDSSFNKQFIENVLLGQWYGYFIVVIMQFFILSYI"
BASE COUNT         3710 a         1836 c         1554 g         3812 t
ORIGIN      
        1 atttataaat agttaattta caactaaacg ataaatatta tatgcaaaat acatctttaa
       61 tattaaagta ataccaatat tttttcaata aacctagtgt aatatatgtg taattctaaa
      121 agattcttct ttaaaaatat aaataccacg acatattgct ttaacatttt catttataaa
      181 gcgaaaaaat gcatcgctac taagttgaat gtttagtaag gatgcattta attcactaaa
      241 atgattaaat tacttatatc ttttcatctg attgattatc gaaatttctt ccttctaaac
      301 ctgctaactc ttctttagaa gctgcaggtg ctttcatttc aaatatctca ttcactactg
      361 tgtaatcgta atatcctaat ctggcaatag gtttaatcga cttaatgtcc aatttaccat
      421 tatcaagaat aaccttatcg tcaatatgaa cttgggcaac tcttcctata acaatatcta
      481 cggtagatac tggatctcca gttggaatac gaatcgtttg aacgtactca cattcaaaat
      541 gaactggcga ttcttttaca cgatatcctg gagcttctat acatttttcc tttgttacat
      601 ctgcaaaatt aaattcatcc tcttctggtg gcaatgcttt cgatgataaa ttaactgctt
      661 ctcttaaatc atacgttgcc atattccaca caaaccaacc tgtctcttca gcatttttca
      721 ctgtatcttt acgttcgtga tcaccaagaa cggattgatt tgctgcgaac ataaccatag
      781 gcggatccca agttaagttt tgatactgac tataaggcgc taaattatct ttcccatctt
      841 tcgatacagt agagatccac cctattggac gtggtactgt actactttta aatgggtcgt
      901 gcggtaaacc atgacttctt acaccttgtt ttggcgaata attcatacta tcttcacccc
      961 ttataagtaa ttacatttaa ggttacgccc tctttacata agcgtctaat ataaataaac
     1021 aatttattta taagtagaaa ctatatatga cgtggttgct tataatttgc gttcttgatt
     1081 cgaaaaattc agataaggat ttatacaatt aatatttatg atatcttttg taaatttaat
     1141 taattatagt tacttcaatc atgattagtt tataataata aagtgaaatt gaaaaagaca
     1201 gctattatgc gatgagcgaa aaacttcaag taaaacaaga atttaacggc gttacaaggc
     1261 aatacttgaa tttcagaaca gatagtatcg atgatctgga taagttcaaa aaagaattca
     1321 aacctgttat gcacttaaaa ggtgatgcat tcaatcaaca gttacaatct ttgattaaca
     1381 aatatccaca aatacaaaaa aatatgaaat cagagttcat tgcttattat gataaagaaa
     1441 aaaatagaga aacagtaaaa aactatgctt ggaaccttca aaaatctata aatgacatta
     1501 tgcaatcata tcctagcaca aaatttgtac agttttataa agatgatgtt tccccgtcaa
     1561 tggtagatgg aaatggccgt ttaaaatcgg atactaatgt aatttccatc gagggaggaa
     1621 agtatgatga gaacaaatga tcatataacc gatttagatg ctattgagtt aagtagcggt
     1681 tggaattaca attcgaaatt ggagactggc aaaaatgcac tactatcaga tagcctttta
     1741 agagatatta aagtattaag ttctaatgct aaaggcgacg ctgacatact agaaacagaa
     1801 cttcgtgctg taataaactc aatgtctcaa aacgaaggta aatctgttga tgcacttaaa
     1861 aatagtaccg cttctctaaa aaacaatcta accaaattaa aagagcaact cgacaaaact
     1921 acacattaat ttcatacatt ttgagtgaac agaattatat ttccatagca aacattccta
     1981 aactacacat acgttacata attgattcat ttttatagaa acgggtaaaa atgataaagt
     2041 aatcatcttt cacaaaaaat caaatattgg gaggaattaa atatgatttt gcgttacgca
     2101 acgaatttaa aacttgcgaa agagctatac caagccagta aacctaaatt gcaagaagat
     2161 aagggtatga ttgaactgtt tgaacatact tttggcttgc aaaaagaact tgtaaaatac
     2221 gtcggcattg cagaagcaac tactgctgct ttatactcag ctagctttat caataaaaac
     2281 atttctcgcc ttgcatcact atcaacgata ggcattttaa gtgtagctgc ttacaaacat
     2341 ttcgaagctg gccaaggtaa aaaaggtgca caacatgcat taaatttatt aggtcttgct
     2401 actttaagtt tactagatac attttcttgt aataaaaaat agtcttcatt ttagaagcac
     2461 atactttagc acctgtgtac ttttgattca attcataaac ggtataccgc tatagcatgt
     2521 gttttattat ttttctggtc tctttatcgt aaatattccc cctaatttag cataatcatt
     2581 tccggctaaa cgagaaacag cctgtaatgc gtgtggattg attttaaaat caccctcata
     2641 aatactgtca tcaatatgat aagttacaat ttcacctatt attaaatcag ccccatctaa
     2701 tacatctcca agcaatatca tttgcgatag tttacattcg aatctcattt tcgcatcttt
     2761 aattcctggc gtcttaatcg ttgtagatgt taaaagtgat aattctgtac gactcaactc
     2821 actgtcacca tatgctaacg gcgctgcagt ctcattaata tcttgaacat tatcttcgtc
     2881 tgtaatatgc acaacaaagt ctccagtccg ttctatattt aatgcagtat cttttctctt
     2941 acctcctgca cgttgaactg caatagcaat cattggcgga tgattattaa caatattaaa
     3001 aaagctaaat ggtgctgcat ttactgatgc atcttgattt aatgttgtaa caaaagctat
     3061 aggtcgtgga ataattgaac caattaataa tttatagttt tctctagcag ttaatgattg
     3121 tgcatcaaac gtatacataa tacctacctc ttttctaagt atatctaggt atttctccga
     3181 ttttggttaa tttaaacatc tattctcctc tgaaaatcac ttgtatttat ttagcaaatc
     3241 ttttgaaata tgacacatat gcatatcttc tggatatttt tctaaatgtt gctgatgttc
     3301 ttcagcactt ttaatgtagt tagacagcgg taagacttcc actgcaattt gatctctgtc
     3361 tttacgtcgt tcaatgaact gacgcgcttc aattaagtgg tcatctacac aactatataa
     3421 acccgttcga tacttttgtc caatatcatt tccttgttga ttcacactgt aaggatcaat
     3481 gatttcaaat aaataattca taatgtctgt aattgttaac atacgatcat cgaaatgaag
     3541 tttgacacat tcagcataac catcatacgg accgtctaat ttagagcttc ttccatttgc
     3601 tcttcctgct tctgtatgta taattccagg tattgttgca aaaaatgctt caacacccca
     3661 taaacatcct cctgctacat aaacaactgc catatttaca cctcatcatc cttttttata
     3721 tttttaacaa ggttatacca tttaataccg ccatgacatg attctgatac accttcatta
     3781 cgatacccat atttttcata aaatgaaatt aatgattctc gacatgttaa cgttacacca
     3841 tgtcgatgat gattcttagc aagagtttca aaatagttta gtaagcgacc tgcaataccc
     3901 tgaccttgat aatttggtgc tacaacaaga cctaacacac taatatagcc accttcacta
     3961 ttatttgtgg agacattttt aaataaatca tcgctaatgt aacgctcttt tatgactgga
     4021 ccgttgatgt atccaattac ttcatcattc ttttcagcta caataaaagt ttcttgaatt
     4081 tgttcaattc tcaatttcaa tgcctctcgt gttgcagctt cttccggtga aaaaccaata
     4141 ttttcaagtg caataatttg atctaagtcc tgcatgatta cttttcttaa taccataaaa
     4201 acacttcctt agccctatat atcaaaagta ataccttcat ctttatattc attattttca
     4261 aactaccttc actataaaaa acgagtcttt ttattgaaag ccattcgcct taatcaatta
     4321 gacaagttgt attatacgaa ttagtaaata atcatgatta taattcattt taagcatact
     4381 caaaaagact ggtacatgta taccagtcta attcgaaaaa atatatttag ttaaaaccat
     4441 ttcaaaaacg acttcacttt tcctggctta gtcggtctgt ctaaataaac aaaattatca
     4501 ttgattactg aactggcatt tcgaagtaat gcttcaatat cattcgaata tttcttcaat
     4561 ttatgattgt gaaataattc ttgcatcaaa aatggtcttt ggtcacatga atgtgcatct
     4621 gaagctacaa aatgagccaa attacattct ataaattgta atgataactt ttgaatgttt
     4681 ttaccaaatc caccaactaa agaactcgat gttaattgac tcagtgcccc atttgcaacc
     4741 aattcatata atatttccgg atttttggcg atacttctat ttctttcagg atgtgcaatg
     4801 attggtatgt aacctctcga ttgtatttca aaaaacaatt gttttgtata atgtggtact
     4861 tcgcccgttg gaaattcaat taataaatat ttcgaacgat taataccttg aatactacca
     4921 ttatctaagc ctttcagaat cgaatctgta attctaattt cttgcccggg aaataattta
     4981 atatccaatg cttgaacttc tggatgcgtt cttaactccg ccaatttcac aagcacttgt
     5041 tgaaatgtat tatcatatct cggatgcaaa tgatgaggtg tcgctacaat acttgttaca
     5101 ccttcatcct tagcttgctt taatagtgca atactctttt caattgtttt aggaccatca
     5161 tctatatcaa ctaatatatg gttatgaata tcaatcatga ttcatcagtc ccataatatg
     5221 catagtaact agcactttta tctttaggca ttctatttaa gactacacct aataatttag
     5281 cacctgttgc ttcaataagt tcttttcctt ttttaacttc atctctatta ttattttccg
     5341 aattaactac gtagacaaca ttgccggtaa actttgaaaa taattgcgca tctgtaactg
     5401 tgttcactgg tggcgtatcg ataattacaa agttataatt catcaataat gtgtcataca
     5461 aatttgcaaa tgcccttgat gtaattaact ctgacggatt cggtgggatt ggcccagacg
     5521 tcaagacgtc taaatcttga atttcagttg agataatact gtcttgataa gttgaccaat
     5581 ttagcaataa acttgatagg ccttcattgt ttggcaaatt aaaaatataa tgctgcgtag
     5641 gtttacgcat atccccgtct acgattagtg ttttataacc tgcttgcgca tatgcaactg
     5701 ctaaatttgc tgcaattgta gacttacctg cgcctggtgc ctctgaagtg attacaatgc
     5761 tttgaactgc actgtcagga tttgcaaaca taatatttga tcttatgcct cgaaatttct
     5821 cgctaatagg tgactttggt tgttcatgga caattaaact tgatgtactt cttcgtgtat
     5881 tcgtcatggt aattcctcgt aaattaaaat ttttgtattg aacctaaaat aggtaatcct
     5941 agttgcgatt caacatcttc ttctgtctta atacgcttat ctaataattc ttttaagaaa
     6001 ataatcaata ttgctaaaac aataccaaca ataatgctga taactaagtt gacagatact
     6061 attggagata cttttacagc attatcatgt gctgaggaaa gtatcgtaac attatcaaca
     6121 ctcataattt taggcatgtc atgagcaaaa actttagata ttttattaac aattttgtca
     6181 gattcagatt tattcccagt ggtaactgat acagtaataa tttgagagtt tgtttgattg
     6241 gttactttta aaaatgaatt caactcagct gttgaatact gaccatcaaa ttctctagat
     6301 actttatcta gaattctagg acttttgata atttccgtat atgtattaac agactgcaaa
     6361 ctactttgaa cattttggaa agctaaatca cttgaggact ttttcatgtt cactaatatt
     6421 tgagtagaag cagtatattt gtcaggcata acaaaaaagg ttaatgccgc acttactaca
     6481 agacatattg ccggtaaaat aagcaataat ttaatattct tctttagaat atttaatagt
     6541 tttactaaat caaacttttc tttcatggtt tcctccacat aatcaatcat tgtattcatt
     6601 atgtatgttt tataaatcgg acaattatat ctagtttaac gaccacaaaa catacacaac
     6661 tacattttct ctaattattt atataaatat tttatcgttt aaaattatat catgattctc
     6721 taccattatg tataacttat ttatattttt gcacaagata taatattgtc caactttaaa
     6781 tatccaaacc tattaataat aaaactagat accatcgtac tctatcatgg ctttcttata
     6841 atcgagtaga agcatcatca ttacttgatt atttgctctt tacaacaccg agcgtgcccg
     6901 tactcggtaa ttcaatacct tgcgtaaccc gtcactgtga gttgggttaa tgataataaa
     6961 gcccacacct tttaaaaaga tgtgggtaat ttatataatt tttatttaca tttttaactt
     7021 ataaaaaaag cgcctatgtc atgatttacc atcacatagg cgcttatcaa taaattatta
     7081 cttattactt tccatttcat ctaatttatg cggatttcct gtaattagat gacaacttat
     7141 tcttttcagg ggaacattac acttttataa tatgttcaaa gacaaactta accattcaca
     7201 aatataaaga ataatattat caaatcattg aacaaatcgt attttgcaac aattgatatt
     7261 tatattaatg tattgcattt aatttataaa attcatatac atcttaatat tctcaatatc
     7321 gatttgtatt gtcaacttta tatagattta aaaaaataat ctcatgtctt tttttacaaa
     7381 agtaagttaa ttattacaaa ctagtaacaa aaattatttc ttcaaaaata tatttagtag
     7441 cgaatacact tcatctttga attgactttt actttcttcc actgctccaa atttttgcga
     7501 aaaggatgct ttcaaatacc aactttcaag aaacagcaat attaaattct gaaagtcttc
     7561 ttttgtcatc tttatctttg attcatcata gaattttgct atctctttac ttaatgattg
     7621 atttaaatct tgtatttgtc cgtaaatatt tccagaaaat tcctcaggcg tattagataa
     7681 ttgaacgtac attctaatat acctttcttc gatgtcgaaa ataaactcaa ataagaattg
     7741 atataaagca tcaattgaat agttcgattt attttgattc atcataataa tattattaag
     7801 gtaatcaaaa caacatttaa cactttgttc gtaaatactt tttttcgagt caaaatggta
     7861 atataaactc gctttcttta tatttacact tttagctata tcatcaagtg ttgtaccgtc
     7921 ataccccttc tctgaaaata aggttattgc gttatcaata atcttatcct tcaattttta
     7981 taacccccta ctgaaaatta atcacactat gttacaggaa aattaagttg caattacaaa
     8041 tatttccgtt taattataac aacaatctat tgcaaattaa aatactatca attaccatat
     8101 ggcttacaac ctaactaacg aaaggtaggt aaagaaattg caatttttta actttttgct
     8161 tttttatcct gtatttatgt ctatttactg gattgtcggt tcaatttatt tctattttac
     8221 cagagaaatt agatattcat tgaacaagaa gcctgacata aatgtggatg aattagaagg
     8281 cattacattt ttacttgcct gttataacga aagtgaaacg attgaagata cgttgtctaa
     8341 tgttcttgca ctcaaatacg agaagaaaga aattattatc attaatgatg gaagttcaga
     8401 taatacagca gaactcatct ataaaatcaa agaaaataat gactttattt tcgtcgattt
     8461 acaagaaaac agaggtaaag ccaacgcact caatcaaggc attaaacagg cttcatatga
     8521 ttatgtaatg tgcttggatg cagatactat cgttgatcaa gatgcaccat attatatgat
     8581 tgagaatttc aaacatgatc caaaacttgg tgcagttaca ggtaatccta gaattcgaaa
     8641 taagagttct attttaggta aaattcaaac gatagaatat gcaagtttaa ttggctgtat
     8701 taagcgaagt cagacacttg ctggcgcagt caatactatt tcgggtgtct tcactctatt
     8761 taaaaaaagt gcagttgtcg acgttggcta ctgggatact gatatgatta ccgaagatat
     8821 tgcagtttct tggaaattgc atttacgtgg atatcgtatt aagtatgaac cgcttgccat
     8881 gtgttggatg ttggttccag aaacattggg aggtctttgg aagcaacgcg tgagatgggc
     8941 tcaaggggga cacgaagtat tactacgaga cttttttagc acaatgaaaa cgaaaaggtt
     9001 tcctttatat attttgatgt ttgagcaaat catctcgatt ttatgggtat atatagtgct
     9061 tctatattta ggctatttgt tcataacagc aaacttctta gactatacat ttatgacata
     9121 tagtttttca atatttctac tatcatcatt tactatgact tttataaacg ttattcaatt
     9181 tacagtcgca ctctttattg atagtcgcta cgagaaaaag aatatggctg gactcatatt
     9241 tgtaagttgg tatccgacag tatactggat tattaacgca gcagtagttc ttgtcgcatt
     9301 tccaaaagca ttaaaacgta agaaaggtgg ttacgcaaca tggtcaagcc cagacagagg
     9361 gaatacccaa cgctaaaatc atcgctaaat attgtaagag aaacagcact tatcgctata
     9421 tcttgtgtct tttggatata ttgtttagtt gttctactcg tttatattgg tactatattt
     9481 gaaattcatg acgaaagtat caatacaata cgtgttgctt taaacattga aaatactgaa
     9541 attttagata tatttgaaac tatgggcatt ttcgcgatta tcatttttgt attttttaca
     9601 attagcatat tgattcaaaa atggcagaga ggaagagaat cgtgaagtat agaaaattta
     9661 taattttagt gttgagtatc ttgatcatat tgcctgtaag cacactggat ggtcatcata
     9721 ttgcaaatgc agatgacgat tcacctaaaa aactgaaata taaagaaaat agtgctctgg
     9781 cattaaatta tcaccgtgta agaaaagcga attttctgaa taattttatt tacttctttt
     9841 ctagtagtaa agaaattaaa aattatagtg ttagtcaatc acaatttgaa tctcaaataa
     9901 aatggctaaa atcacatgat gctaaatttt taaccttgaa agaattttta tattacaaga
     9961 aaaaaggtaa gtttccaaaa cgaagtgtat ggattaactt tgatgatatg gatgaaacta
    10021 tttatgaaaa tgcttatcca atcttaaaaa aatataaaat accggcaact gggtttatta
    10081 tcacaggtca tgttggggaa gaaaactttc acaacctcga tatgattagt aaaaaagaac
    10141 taaaagaaat gtataaaact gggttatggg aatttgaaac acatacccac gatttgcata
    10201 acttatctaa aaataataag tcaaaattaa tgaaagcttc tgaagctaca atcataaaag
    10261 atttaaacaa aagtgaaaaa tatctaacta aaaactttaa aaagtcgcag aaaactatag
    10321 cctatcctta tggcttgatg aatgacgata aattaccggt aatcaaaaaa gctgggttaa
    10381 aatacggttt ttcattagag gaaaaagcag tcactccgaa ctccaatgat tattacatcc
    10441 ctagaatatt aattagtgat gatgcttttg agcatttaat taagagatgg gacggattcc
    10501 atgaaaaaga ttagacttga actcgtatat ttacgtgcta ttatatgtgc aattattatt
    10561 atcacacatt tacttacaca aattacttta aaacatgaaa atatggaggg tggatcctta
    10621 gtgttacaat tttacattcg taatattgtg atttttggta caccttgctt tattatcttg
    10681 tcacagttac tgacaacctt gaattaccaa aaagtcacct atagatactt aactacacgc
    10741 gtaaaatata tacttattcc ttacatatta atgggattgt tttacagtta tagtgaatca
    10801 ttattaacag attcaagttt caataaacaa ttcattgaaa atgtcctatt aggtcaatgg
    10861 tatggctatt ttatcgttgt tatcatgcaa ttctttattt tgagttatat ca
//
LOCUS       DALAGD010000095        10505 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.096, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000095 DALAGD010000000
VERSION     DALAGD010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10505
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.096"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            132..1661
                     /gene="aur"
                     /locus_tag="O2701_002207"
     CDS             132..1661
                     /gene="aur"
                     /locus_tag="O2701_002207"
                     /EC_number="3.4.24.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloproteinase aureolysin"
                     /protein_id="HCZ4294084.1"
                     /translation="MRKFSRYAFTSMAALTLLSTLSPAALAIDSKNKPANSDIKFEVT
                     QKSDAVKALKELPKSENVKNIYQDYAVTDVKTDKKGFTHYTLQPSVDGVHAPDKEVKV
                     HADKSGKVVLINGDTDAKKVKPTNKVTLSKDDAADKAFKAVKIDKNKAKNLKDKVIKE
                     NKVEIDGDSNKYVYNVELITVTPEISHWKVKIDAQTGEILEKMNLVKEAAETGKGKGV
                     LGDTKDININSIDGGFSLEDLTHQGKLSAFSFNDQTGQATLITNEDENFVKDEQRAGV
                     DANYYAKQTYDYYKDTFGRESYDNQGSPIVSLTHVNNYGGQDNRNNAAWIGDKMIYGD
                     GDGRTFTSLSGANDVVAHELTHGVTQETANLEYKDQSGALNESFSDVFGYFVDDEDFL
                     MGEDVYTPGKEGDALRSMSNPEQFGQPAHMKDYVFTEKDNGGVHTNSGIPNKAAYNVI
                     QAIGKSKSEQIYYRALTEYLTSNSNFKDCKDALYQAAKDLYDEQTAEQVYEAWNEVGV
                     E"
     gene            2028..2477
                     /locus_tag="O2701_002208"
     CDS             2028..2477
                     /locus_tag="O2701_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCZ4294085.1"
                     /translation="MKKSKRLEIVSTIVKKHKIYKKEQIISYIEEYFGVRYSATTIAK
                     DLKELNIYRVPIDCETWIYKAINNQTEQEMREKFRHYCEHEVLSSIINGSYIIVKTSP
                     GFAQGINYFIDQLNIEEILGTVSGNDTTLILTASNDMAEYVYAKLFK"
     gene            2480..2647
                     /locus_tag="O2701_002209"
     CDS             2480..2647
                     /locus_tag="O2701_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294086.1"
                     /translation="MYQMNNKNLFRITCTQVSYQILTCVRVFLWKERIHSHSIDCIKN
                     FIQHVFMGVFE"
     gene            2824..4059
                     /gene="arcA"
                     /locus_tag="O2701_002210"
     CDS             2824..4059
                     /gene="arcA"
                     /locus_tag="O2701_002210"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCZ4294087.1"
                     /translation="MTDGPIKVNSEIGALKTVLLKRPGKEIENLVPDYLDGLLFDDIP
                     YLEVAQKEHDHFAQVLREEGVEVLYLEKLAAESIENPQVRSEFIDDVLAESKKTILGH
                     EEEIKALFATLSNQELVDKIMSGVRKEEINPKCTHLVEYMDDKYPFYLDPMPNLYFTR
                     DPQASIGHGITINRMFWRARRRESIFIQYIVKHHPRFKDANIPIWLDRDCPFNIEGGD
                     ELVLSKDVLAIGVSERTSAQAIEKLARRIFENPQATFKKVVAIEIPTSRTFMHLDTVF
                     TMIDYDKFTMHSAILKAEGNMNIFIIEYDDVNKDIAIKQSSHLKDTLEDVLGIDDIQF
                     IPTGNGDVIDGAREQWNDGSNTLCIRPGVVVTYDRNYVSNDLLRQKGIKVIEISGSEL
                     VRGRGGPRCMSQPLFREDI"
     gene            4092..5102
                     /gene="argF"
                     /locus_tag="O2701_002211"
     CDS             4092..5102
                     /gene="argF"
                     /locus_tag="O2701_002211"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCZ4294088.1"
                     /translation="MTEIQKPYDLKGRSLLKESDFTKAEFEGLIDFAITLKEYKKNGI
                     KHHYLSGKNIALLFEKNSTRTRAAFTVASIDLGAHPEFLGKNDIQLGKKESVEDTAKV
                     LGRMFDGIEFRGFSQQAVEDLAKFSGVPVWNGLTDDWHPTQMLADFMTIKENFGYLEG
                     INLTYVGDGRNNIAHSLMVAGAMLGVNVRICTPKSLNPKEAYVDIAKEKASQYGGSVM
                     ITDNIAEAVENTDAIYTDVWVSMGEESEFEQRINLLKDYQVNQQMFDLTGKDSTIFLH
                     CLPAFHDTNTLYGQEIYEKYGLAEMEVTDQIFRSEHSKVFDQAENRMHTIKAVMAATL
                     GS"
     gene            5193..6623
                     /gene="arcD"
                     /locus_tag="O2701_002212"
     CDS             5193..6623
                     /gene="arcD"
                     /locus_tag="O2701_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HCZ4294089.1"
                     /translation="MNESGDNKLSKSSLIGLVIGSMIGGGAFNIMSDMGGKAGGLAII
                     IGWIITAIGMISLAFVFQNLTNERPELDGGIYSYAQAGFGDFVGFISAWGYWFSAFLG
                     NVAYATLLMSAVGNFFPIFKGGNTLPSVIVASLLLWGVHFLILKGVETAAFINSIVTV
                     AKLIPILLVIICMIIAFNFDTFKTGFFSMTSEGVLPFSWASTMSQVKSTMLVTVWVFI
                     GIEGAVIFSSRAKNKKDVGSATVIGLISVLIIYFLLTVLAQGVILQNHISQLDSPSMA
                     QVLATIVGGWGSTLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNG
                     APVNALLITNILVQLFLISMLFTQSAYQFAFSLASSAILYPYMFSAFYQVKYTLEHRQ
                     QATTKQWTIGIIASIYAIWLIYAAGINYLLLTMLLYIPALLVYTIVQKNNQTRLIKSD
                     YILFMIIIVLAVIGLIKLLMGTINVF"
     gene            6640..7581
                     /gene="arcC"
                     /locus_tag="O2701_002213"
     CDS             6640..7581
                     /gene="arcC"
                     /locus_tag="O2701_002213"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCZ4294090.1"
                     /translation="MKEKIVIALGGNAIQTTEATAEAQQTAIRCAMQNLKPLFDSPAR
                     IVISHGNGPQIGSLLIQQAKSNSDTTPAMPLDTCGAMSQGMIGYWLETEINRILTEMN
                     SDRTVGTIVTRVEVDKDDPRFDNPTKPIGPFYTKEEVEELQKEQPDSVFKEDAGRGYR
                     KVVASPLPQSILEHQLIRTLADGKNIVIACGGGGIPVIKKENTYEGVEAVIDKDFASE
                     KLATLIEADTLMILTNVENVFINFNEPNQQQIDDIDVATLKKYAAQGKFVEGSMLPKI
                     EAAIRFVESGENKKVIITNLEQAYEALIGNKGTHIHM"
     gene            7680..8384
                     /locus_tag="O2701_002214"
     CDS             7680..8384
                     /locus_tag="O2701_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCZ4294091.1"
                     /translation="MTENFILGRNNKLEHELKALADYINIPYSILQPYQSECFVRHYT
                     KGQVIYFSPQESSNIYFLIEGNIIREHYNQNGDVYRYFNKEQVLFPISNLFHPKEVNE
                     LCTALTDCTVLGLPRELMAFLCKANDDIFLTLFALINDNEQQHMNYNMALTSKFAKDR
                     IIKLICHLCQTVGYDQDEFYEIKQFLTIQLMSDMAGISRETAGHIIHELKDEKLVVKD
                     HKNWLVSKHLFNDVCV"
     gene            8734..>10505
                     /gene="clfB"
                     /locus_tag="O2701_002215"
     CDS             8734..>10505
                     /gene="clfB"
                     /locus_tag="O2701_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfB"
                     /protein_id="HCZ4294092.1"
                     /translation="MKKRIDYLSNKQNKYSIRRFTVGTTSVIVGATILFGIGNHQAQA
                     SEQSNDTTQSSKNNASADSEKNNMIETPQLNTTANDTSDISANTNSANVDSTTKPMST
                     QTSNTTTTEPASTNETPQPTAIKNQATAAKMQDQTVPQEANSQVDNKTTNDANSIATN
                     SELKNSQTLDLPQSSPQTISNAQGTSKPSVRTRAVRSLAVAEPVVNAADAKGTNVNDK
                     VTASNFKLEKTTFDPNQSGNTFMAANFTVTDKVKSGDYFTAKLPDSLTGNGDVDYSNS
                     NNTMPIADIKSTNGDVVAKATYDILTKTYTFVFTDYVNNKENINGQFSLPLFTDRAKA
                     PKSGTYDANINIADEMFNNKITYNYSSPIAGIDKPNGANISSQIIGVDTASGQNTYKQ
                     TVFVNPKQRVLGNTWVYIKGYQDKIEESSGKVSATDTKLRIFEVNDISKLSDSYYADP
                     NDSNLKEVTDQFKNRIYYEHPNVASIKFGDITKTYVVLVEGHYDNTGKNLKTQVIQEN
                     VDPVTNRDYSIFGWNNENVVRYGGGSADGDSAVNPKDPTPGPPVDPEPSPDPEPEPSP
                     DPDPDSDSDSDSGSDSDSGSDSDS"
BASE COUNT         3911 a         1496 c         2004 g         3094 t
ORIGIN      
        1 gaaaagaatc aataaataaa atcaacgaac aaaaagtata gaaataaata gaaataatca
       61 tttacttttc tgaaaaatta aattaatatt ttatttataa gctgttttta agatttcagg
      121 aggaatgaaa tgtgaggaaa ttttcaagat atgcatttac aagtatggca gcattaacct
      181 tgttgagcac tttatcacca gcagcattag cgattgattc aaaaaataaa ccagctaatt
      241 ctgatattaa atttgaggtg actcaaaaga gtgatgcggt caaagcatta aaagaattgc
      301 ctaaatccga aaatgtaaaa aatatttatc aagattacgc tgttactgat gtaaaaactg
      361 ataaaaaagg atttacgcat tatacattgc aaccgagtgt tgatggtgtt catgcacctg
      421 acaaagaagt gaaagtacac gcagacaaat caggaaaagt cgttttaatc aatggggata
      481 ctgatgcgaa gaaagtaaag ccaacgaata aagtgacatt aagtaaagat gacgcagccg
      541 acaaagcatt taaagcagtt aagattgata agaataaagc gaaaaatctt aaagataaag
      601 tcattaaaga aaacaaagtt gaaatcgatg gtgacagtaa taaatacgtt tataatgttg
      661 agttaattac agtgacacca gaaatttcac attggaaagt taaaattgat gctcaaactg
      721 gcgaaatttt agaaaaaatg aacttagtta aagaagctgc agaaactggt aaaggaaaag
      781 gtgtacttgg cgatacaaaa gatatcaata tcaatagtat tgacggtgga tttagcctag
      841 aagatttaac gcatcaaggt aaattatcag catttagctt taatgatcaa acaggtcaag
      901 caacattgat tactaatgaa gatgaaaact tcgtaaaaga tgagcaacgt gctggcgtag
      961 atgcaaatta ttacgctaaa caaacatatg attattacaa agacacattt ggtcgtgaat
     1021 catatgacaa ccaaggtagt ccaattgttt cattaacgca tgttaataac tacggtggtc
     1081 aagataacag aaataatgcc gcatggatcg gtgacaaaat gatctatggt gatggtgatg
     1141 gtcgcacatt cacaagttta tcgggtgcaa atgacgtagt agcacacgaa ttaacacacg
     1201 gtgtgacaca agagacagcg aacttagaat ataaggacca gtcaggcgct ctaaatgaaa
     1261 gcttttcaga tgtttttgga tactttgtag atgacgagga tttcttaatg ggtgaagatg
     1321 tctacacacc tggaaaagag ggagacgctt tacgcagcat gtcaaaccca gaacaatttg
     1381 gtcaaccagc tcatatgaaa gactatgtat tcactgaaaa agataatggt ggcgtacata
     1441 cgaattctgg aattccaaat aaagcagctt ataacgtgat tcaagcaata gggaaatcta
     1501 aatcagaaca aatttactac cgagcattaa cggaatactt aacaagtaat tcaaacttca
     1561 aagattgtaa agatgcatta taccaagcgg ctaaagattt atatgacgag caaacagctg
     1621 aacaggtgta tgaagcatgg aatgaagtag gcgtggagta aaaatatata aacaagaaga
     1681 agtaatgtta aacacttata aataattaaa ttttaaatac agcttatata tggggtatac
     1741 ctacattgat aattgacttt gtaggtatac cctctctttg taattagatg agaggagtgt
     1801 gagggttgtc tgccgaaaga ctactcggca gtctaaaatc attacaagta gtagatatgt
     1861 gataattaaa tgctgactta gaatacaaaa ttcattttaa aagttgtcac aaaaaattta
     1921 catgtatttt tattatattt tgcaaaacaa agtgttaaat tataaatgaa acatgcatga
     1981 atttattttt taatacaaga aacgtaacta ccaaaggagt ttacaatatg aagaaaagta
     2041 aacgattaga aattgtttct acaatagtta aaaagcataa gatttataaa aaagaacaaa
     2101 tcatttcata tattgaagaa tattttggtg taagatatag tgcaacaaca attgctaaag
     2161 acttgaagga actaaatata tatcgtgtcc ctatcgattg tgagacatgg atttataaag
     2221 ctattaataa tcaaacagaa caagagatga gagaaaagtt tagacactat tgtgaacatg
     2281 aagttctaag ttcaatcatc aatggttcat acattatcgt caaaacctca cctggtttcg
     2341 cccaaggcat aaactatttt atcgatcagc taaatataga agagatatta ggtacggtga
     2401 gtggaaatga cactacatta atcttaactg cctcaaatga tatggcagaa tacgtatatg
     2461 caaaattatt taaatagaca tgtatcaaat gaataataaa aatttgtttc gtatcacgtg
     2521 tactcaagtt agttaccaaa tattaacttg tgtacgcgtt tttttatgga aagaaagaat
     2581 tcatagtcat tcaattgact gtataaaaaa ctttatacaa catgttttta tgggtgtttt
     2641 tgaataaaaa atgtatattt tgacccaaaa tgcctttatt tatgtataaa aatccattat
     2701 tatgtattgt ataacaaaaa gatatgaaat tttcgacttt ctttatgtga atataatcac
     2761 atgtaagcgt ttgaagattg tctatactct aaatgaattc aaagataaaa ggaggaaata
     2821 gacatgacag atggtccaat taaagtaaat agcgaaattg gagctttaaa aactgtgtta
     2881 cttaagcgtc ctggaaaaga aatagaaaat ttagtacctg attatttaga tggattacta
     2941 tttgatgata ttccatattt agaagtagct caaaaagagc atgaccattt tgcgcaggtg
     3001 ctaagagaag agggtgttga agtactttac cttgagaagt tagcagctga aagtattgaa
     3061 aatcctcaag taagaagtga atttattgat gatgtattag cagagtctaa aaaaacaata
     3121 ttaggtcatg aagaagaaat taaggcatta tttgcgacac tttctaatca agaacttgta
     3181 gataaaataa tgtcaggggt acgtaaggaa gaaattaatc cgaaatgtac acatctagta
     3241 gagtatatgg atgataagta tccattctat ttagatccaa tgccaaacct ttattttact
     3301 agagatccac aagcctcaat aggacacggt ataacaatca atcggatgtt ctggagagca
     3361 cgacgacgag aatcaatatt tattcaatat attgtaaagc atcatcctag atttaaagat
     3421 gcgaatattc caatctggtt agatcgagat tgcccattca atattgaagg cggcgatgaa
     3481 cttgttttat ctaaagatgt cttggctata ggcgtttcag aacgtacatc tgcacaagct
     3541 attgaaaagt tagcgcgacg tatttttgaa aatccgcagg cgacgtttaa aaaagtagta
     3601 gcaattgaaa ttccaactag tcgaactttt atgcacttag atacagtatt tacaatgata
     3661 gattatgaca aatttacaat gcattcagcc attttaaagg cagaaggcaa tatgaatata
     3721 tttattattg aatatgatga cgtaaataaa gatattgcca tcaaacaatc tagtcattta
     3781 aaagatactt tagaagacgt actaggtata gatgatatcc aattcattcc aacaggaaat
     3841 ggtgatgtca ttgatggtgc tagagagcaa tggaatgatg gctcaaatac attatgtata
     3901 agaccaggcg ttgtagtgac ttacgataga aactatgtat cgaatgattt attgagacaa
     3961 aaaggcatta aagtcattga aatatctggt agcgagttgg tacgtggacg tgggggccct
     4021 agatgtatga gtcaaccatt attcagagaa gacatttaac gtaataaatt atagaaacga
     4081 gggtgaaaat aatgacagaa attcaaaaac cgtatgattt aaaaggcaga tcattattaa
     4141 aagaaagtga ttttaccaaa gcagaattcg aaggacttat tgattttgca attacattaa
     4201 aagagtataa gaaaaacggt attaagcatc actacttatc tggaaaaaat attgcactac
     4261 tattcgaaaa gaattcgacg agaacgcgtg ctgcgtttac agttgcgtct attgatttag
     4321 gtgcgcatcc agaattttta ggaaaaaatg atattcaatt aggcaaaaaa gaatctgtag
     4381 aggatactgc gaaagtatta ggtagaatgt tcgatggtat tgaattccgt ggtttttcac
     4441 aacaagctgt tgaagattta gcgaagttct ctggtgtacc ggtgtggaat ggattaacag
     4501 acgattggca tcctacacaa atgttagctg attttatgac aataaaagag aattttggat
     4561 atctagaagg aataaactta acttacgttg gagatggacg taataatatt gcgcattcat
     4621 taatggtagc aggtgctatg ttaggtgtta atgtaagaat ttgtacacct aaatcattaa
     4681 atccaaaaga ggcatatgtt gatattgcaa aagaaaaagc gagtcaatat ggtggttcag
     4741 tcatgattac ggataatatt gcagaagcag ttgaaaatac agatgctata tatacagatg
     4801 tttgggtatc gatgggtgaa gaaagtgaat ttgaacaacg tattaattta ttaaaagact
     4861 atcaagtgaa tcaacagatg tttgatttaa caggtaaaga ttcaacgata ttcttacatt
     4921 gtttaccagc attccatgat acaaatacac tttatggaca agaaatttat gaaaaatatg
     4981 gattagctga aatggaagtt acagaccaaa tctttagaag tgaacattca aaagtgtttg
     5041 atcaagctga aaatagaatg catacaatta aggcagtaat ggcagcaaca ttggggagtt
     5101 aatcactaaa tggaacgata tgaatatgat gtgtctgatg atataagtgt catgtacaga
     5161 cacctcatat tggtattaaa ggagaaatga atatgaacga atcaggagat aacaaactca
     5221 gtaaatcttc tttaattgga ctagttatag gatccatgat tggtggcggt gcgttcaata
     5281 taatgtctga tatgggcggt aaagccggtg gattagccat tattattggt tggattatta
     5341 cagctatagg aatgatttca ttagcgttcg tatttcaaaa tttaaccaat gaacggccgg
     5401 agctagacgg tggtatttat agttatgctc aagcaggatt tggcgatttt gtaggattta
     5461 tcagtgcttg gggatattgg ttctcagcgt ttttaggcaa tgttgcctat gcaacactat
     5521 tgatgtcagc agtaggtaac tttttcccga tttttaaagg aggcaacaca ttaccaagtg
     5581 ttattgtcgc ctcgttacta ctctggggtg tccatttctt gattttaaaa ggcgttgaaa
     5641 cagcagcatt tatcaatagt attgttactg ttgcaaagtt aataccgatt ttacttgtaa
     5701 tcatatgcat gataattgca ttcaattttg acacttttaa aacaggcttt ttcagtatga
     5761 cgtcagaggg tgtattgcca tttagttggg cgagcacaat gagccaagtt aaaagtacga
     5821 tgctagtgac agtttgggtg tttatcggta tcgaaggtgc agtaattttt tctagtagag
     5881 ctaaaaataa aaaagatgta ggtagtgcca cggttatagg acttatatca gttttaatta
     5941 tctatttctt attaactgta ttagctcaag gcgtgatttt gcaaaatcat atttcgcaat
     6001 tagattcgcc aagtatggca caggtgcttg caactattgt aggtggttgg ggatctacac
     6061 ttgtaaatat tggtttaatt atttcggtac taggtgcatg gttaggatgg acactgcttg
     6121 ctggtgaatt acctttcatt gttgcaaaag atggattatt tccaaaatgg tttgctaaag
     6181 aaaataaaaa tggagcacct gtaaatgcac tgcttattac caatatatta gtacaattat
     6241 ttttaataag tatgctattt acacagagtg cgtatcaatt tgcattttca ctagcatcaa
     6301 gtgctatttt atacccttac atgttcagtg cattttacca agttaaatac actttagagc
     6361 atcgacagca agcaactact aaacaatgga cgattggtat catagcctca atttatgcta
     6421 tatggcttat atatgcagca ggtatcaatt acttattatt gacgatgtta ctttatattc
     6481 cagctcttct tgtttataca atcgttcaaa agaataatca gacacgtttg attaaatcag
     6541 actatattct ttttatgatt attatcgtac ttgcagttat cgggttaatt aagttattga
     6601 tgggaacgat aaatgttttt taaaaggagc gacaaaaata tgaaagagaa aattgtcatt
     6661 gcattaggcg gtaatgcgat acagacaaca gaagcaacag ctgaagcaca acaaacagct
     6721 attagatgtg cgatgcaaaa ccttaaacct ttatttgatt caccagcgcg tattgtcatt
     6781 tcacatggta atggtccaca aattggaagt ttattaatcc aacaagctaa atcgaacagt
     6841 gacacaacgc cggcaatgcc attggatact tgtggtgcaa tgtcacaggg tatgataggc
     6901 tattggttgg aaactgaaat caatcgcatt ttaactgaaa tgaatagtga tagaactgta
     6961 ggcacaatcg ttacacgtgt ggaagtagat aaagatgatc cacgatttga taacccaact
     7021 aaaccaattg gtccttttta tacgaaagaa gaagttgaag aattacaaaa agaacagcca
     7081 gactcagtct ttaaagaaga tgcaggacgt ggttatagaa aagtagttgc gtcaccacta
     7141 cctcaatcta tactagaaca ccagttaatt cgaactttag cagacggtaa aaatattgtc
     7201 attgcatgcg gtggtggcgg tattccagtt ataaaaaaag aaaataccta tgaaggtgtt
     7261 gaagcggtta tagataaaga ttttgctagt gagaaattag caacgctgat tgaagcagat
     7321 accttaatga ttcttacgaa tgtagaaaat gtatttatta actttaatga acctaatcaa
     7381 caacaaatcg atgatattga tgtagcaaca ctgaaaaaat acgcggcaca aggtaagttt
     7441 gtggaaggat cgatgttgcc aaaaatagaa gctgcgatac gatttgttga aagtggggaa
     7501 aacaaaaaag ttatcattac caatttagag caggcatacg aagctttgat tggtaataaa
     7561 ggtacacaca ttcacatgta gcactttatc acgcgacaaa acattaaata tgtttctccg
     7621 ttgattcaaa tgaaaaagtt gtctgctgac actttgcaag gtttgaagga gtttaactta
     7681 tgacagaaaa ctttattttg ggtagaaata ataaattaga acatgaacta aaggcattag
     7741 cagattacat taatatacca tatagtatat tacaaccata tcaaagtgaa tgttttgtca
     7801 gacattatac gaaaggccaa gttatttatt tttcgccaca agaaagtagc aatatttact
     7861 ttttaattga aggtaacatt attagagaac attacaatca aaatggagat gtatatcgtt
     7921 attttaataa agagcaagta ttatttccaa tcagtaactt atttcatccg aaagaggtta
     7981 acgaattgtg tacagcatta accgattgta cagttcttgg attgcctaga gaattgatgg
     8041 cctttttgtg caaagctaat gatgatatat ttttgacact ttttgcatta ataaatgata
     8101 atgagcagca acacatgaac tataacatgg cattaacaag taaatttgct aaagatcgaa
     8161 ttatcaaatt gatatgccat ctatgtcaga cagtaggata cgatcaagat gaattttatg
     8221 aaatcaaaca gtttttaact attcaactca tgagtgatat ggctgggatt tcccgggaaa
     8281 cagctggtca tattattcat gaacttaaag atgaaaaact tgttgttaaa gatcataaaa
     8341 attggttagt aagcaaacat ttattcaatg atgtatgtgt ttaatataca atgtaaaatg
     8401 aataagttga acatgaggtc taacgtacat ttatacgtta ggcctttttt gctagcatga
     8461 tgaataattt aaaatgttag ttaaatttga ttgttgaaat tacagtaaaa tttaaggtga
     8521 tgaaaaattt agaacttcta agtttttgaa aagtaaaaaa tttgtaatag tgtaaaaata
     8581 gtatattgat ttttgctagt taacagaaaa ttttaagtta tataaatagg aagaaaacaa
     8641 attttacgta atttttttcg aaaagcaatt gatataattc ttatttcatt atacaattta
     8701 gactaatcta gaaattgaaa tggagtaata tttttgaaaa aaagaattga ttatttgtcg
     8761 aataagcaga ataagtattc gattagacgt tttacagtag gtaccacatc agtaatagta
     8821 ggggcaacta tactatttgg gataggcaat catcaagcac aagcttcaga acaatcgaac
     8881 gatacaacgc aatcttcgaa aaataatgca agtgcagatt ccgaaaaaaa caatatgata
     8941 gaaacacctc aattaaatac aacggctaat gatacatctg atattagtgc aaacacaaac
     9001 agtgcgaatg tagatagcac aacaaaacca atgtctacac aaacgagcaa taccactaca
     9061 acagagccag cttcaacaaa tgaaacacct caaccgacgg caattaaaaa tcaagcaact
     9121 gctgcaaaaa tgcaagatca aactgttcct caagaagcaa attctcaagt agataataaa
     9181 acaacgaatg atgctaatag catagcaaca aacagtgagc ttaaaaattc tcaaacatta
     9241 gatttaccac aatcatcacc acaaacgatt tccaatgcgc aaggaactag taaaccaagt
     9301 gttagaacga gagctgtacg tagtttagct gttgctgaac cggtagtaaa tgctgctgat
     9361 gctaaaggta caaatgtaaa tgataaagtt acggcaagta atttcaagtt agaaaagact
     9421 acatttgacc ctaatcaaag tggtaacaca tttatggcgg caaattttac agtgacagat
     9481 aaagtgaaat caggggatta ttttacagcg aagttaccag atagtttaac tggtaatgga
     9541 gacgtggatt attctaattc aaataatacg atgccaattg cagacattaa aagtacgaat
     9601 ggcgatgttg tagctaaagc aacatatgat atcttgacta agacgtatac atttgtcttt
     9661 acagattatg taaataataa agaaaatatt aacggacaat tttcattacc tttatttaca
     9721 gaccgagcaa aggcacctaa atcaggaaca tatgatgcga atattaatat tgcggatgaa
     9781 atgtttaata ataaaattac ttataactat agttcgccaa ttgcaggaat tgataaacca
     9841 aatggcgcga acatttcttc tcaaattatt ggtgtagata cagcttcagg tcaaaacaca
     9901 tacaagcaaa cagtatttgt taaccctaag caacgagttt taggtaatac gtgggtgtat
     9961 attaaaggct accaagataa aatcgaagaa agtagcggta aagtaagtgc tacagataca
    10021 aaactgagaa tttttgaagt gaatgatata tctaaattat cagatagcta ctatgcagat
    10081 ccaaatgact ctaaccttaa agaagtaaca gaccaattta aaaatagaat ctattatgag
    10141 catccaaatg tagctagtat taaatttggt gatattacta aaacatatgt agtattagta
    10201 gaagggcatt acgacaatac aggtaagaac ttaaaaactc aggttattca agaaaatgtt
    10261 gatcctgtaa caaatagaga ctacagtatt ttcggttgga ataatgagaa tgttgtacgt
    10321 tatggtggtg gaagtgctga tggtgattca gcagtaaatc cgaaagaccc aactccaggg
    10381 ccgccggttg acccagaacc aagtccagac ccagaaccgg aaccaagccc agacccggat
    10441 ccggattcgg attcagacag tgactcaggc tcagacagcg actcaggttc agatagcgac
    10501 tcaga
//
LOCUS       DALAGD010000096        10404 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.097, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000096 DALAGD010000000
VERSION     DALAGD010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10404
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.097"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(19..273)
                     /locus_tag="O2701_002216"
     CDS             complement(19..273)
                     /locus_tag="O2701_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294093.1"
                     /translation="MLIKIRGGNMKRDFFEKWSFILNLILFCAFLFLVVLMFYKDVDL
                     IYIFCTLLISLLPLLNIIKRIGWEINQSSMIYEVILAVID"
     gene            complement(429..1337)
                     /locus_tag="O2701_002217"
                     /pseudo
     CDS             complement(429..1337)
                     /locus_tag="O2701_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002440612.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            complement(1562..1774)
                     /locus_tag="O2701_002218"
                     /pseudo
     CDS             complement(1562..1774)
                     /locus_tag="O2701_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc peptidase"
     gene            complement(1849..3384)
                     /locus_tag="O2701_002219"
     CDS             complement(1849..3384)
                     /locus_tag="O2701_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCZ4294094.1"
                     /translation="MSNIAFYVVSDVHGYIFPTDFTSRNQYQPMGLLLANHVIEQDRR
                     QYDQSFKIDNGDFLQGSPFCNYLIAHSGSSQPLVDFYNRMAFDFGTLGNHEFNYGLPY
                     LKDTLRRLNYPVLCANIYENDSTLTDNGVKYFQVGDQTVGVIGLTTQFIPHWEQPEHI
                     QSLTFHSAFEILQQYLPEMKRHADIIVVCYHGGFEKDLESGTPTEVLTGENEGYAMLE
                     AFSKDIDIFITGHQHRQIAERFKQTAVIQPGTRGTTVGRVVLSTDEYENLSVESCELL
                     PVIDDSTFTIDEDDQHLRKQLEDWLDYEITTLPYDMTINHAFEARVAPHPFTNFMNYA
                     LLEKSDADVACTALFDSASGFKQVVTMRDVINNYPFPNTFKVLAVSGAKLKEAIERSA
                     EYFDVKNDEVSVSADFLEPKPQHFNYDIYGGVSYTIHVGRPKGQRVSNMMIQGHAVDL
                     KQTYTICVNNYRAVGGGQYDMYIDAPVVKDIQVEGAQLLIDFLSNNNLMRIPQVVDFK
                     VEK"
     gene            complement(3435..4979)
                     /locus_tag="O2701_002220"
     CDS             complement(3435..4979)
                     /locus_tag="O2701_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCZ4294095.1"
                     /translation="MKKIYKSLTVSAIVATVSLSALPQSLAITHESQPTKQQRTVLFD
                     RSHGQTAGAADWVSDGAFSDYADSIQKQGYDVKAIDGHSNITEASLKSSKIFVIPEAN
                     IPFKESEQAAIVKYVKQGGNVVFISDHYNADRNLNRIDSSEAMNGYRRGAYEDMSKGM
                     NAEEKSSTAMQGVKSSDWLSTNFGVRFRYNALGDLNTSNIVSSKESFGITEGVKSVSM
                     HAGSTLAITNPEKAKGIVYTPEQLPAKSKWSHAVDQGIYNGGGKAEGPYVAISKVGKG
                     KAAFIGDSSLVEDSSPKYVREDNGEKKKTYDGFKEQDNGKLLNNITAWMSKDNDGKSL
                     KASSLTLDTKTKLLDFERPERSTEPEKEPWSQPPSGYKWYDPTTFKAGSYGSEKGADP
                     QPNTPDDHTPPNQNEKVTFDIPQNVSVNEPFEMTIHLKGFEANQTLENLRVGIYKEGG
                     RQIGQFSSKDNDYNPPGYSTLPTVKADENGNVTIKVNAKVLESMEGSKIRLKLGDKTL
                     ITTDFK"
     gene            5208..6164
                     /locus_tag="O2701_002221"
     CDS             5208..6164
                     /locus_tag="O2701_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCZ4294096.1"
                     /translation="MKNFKCLFVLMLAVIVFAAACGNSSSLDNQKNASNDSDSKSGGY
                     KPKELTVQFVPSQNAGTLEAKAKPLEKLLSKELGIPVKVSVSTNYNTIVEAMKSKKVD
                     VGFLPPTAYTLAHDQKAADLLLQAQRFGVKEDGSASKELVDSYKSEILVKKDSKIKSL
                     KDLKGKKIALQDVTSTAGYTFPLAMLKNEAGINATKDMKIVNVKGHDQAVISLLNGDV
                     DAAAVFNDARNTVKKDQPNVFKDTRILKLTQAIPNDTISVRPDMDKDFQEKLKKAFID
                     IAKSKEGHKIISEVYSHEGYTETKDSNFDIVREYEKLVKDMK"
     gene            6378..7151
                     /gene="phnC"
                     /locus_tag="O2701_002222"
     CDS             6378..7151
                     /gene="phnC"
                     /locus_tag="O2701_002222"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCZ4294097.1"
                     /translation="MSQIEFKNVSKVYPNGHVGLKNINLNIEKGEFAVIVGLSGAGKS
                     TLLRSVNRLHDITSGEIFIQGKSITKAHGKALLEMRRNIGMIFQHFNLVKRSSVLRNV
                     LSGRVGYHPTWKMVLGLFPKEDKIKAMDALERVNILDKYNQRSDELSGGQQQRISIAR
                     ALCQESEIILADEPVASLDPLTTKQVMDDLRKINQELGITILINLHFVDLAKEYGTRI
                     IGLRDGEVVYDGPASEATDDVFSEIYGRTIKEDEKLGVN"
     gene            7153..7953
                     /gene="phnE"
                     /locus_tag="O2701_002223"
     CDS             7153..7953
                     /gene="phnE"
                     /locus_tag="O2701_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCZ4294098.1"
                     /translation="MPLEIPTKYDSLLKKKVSLKTSFTFMLIIVLIIWSFIYTGFNFG
                     DLMIGIPQIGDLFKQMIPPDFEYLQQITTPMLDTIRMAIVSTVLGSIVSIPIALLCAS
                     NIVHQKWISIPSRFILNIVRTIPDLLLAAIFVAVFGIGQIPGILALFILTICIIGKLL
                     YESLETIDPGPMEAMTAVGANKIKWIVFGVVPQAISSFMSYVLYAFEVNIRASAVLGL
                     VGAGGIGLFYDQTLGLFQYPKTATIILFTLVIVVVIDYISTKVRAHLA"
     gene            7950..8765
                     /gene="phnE"
                     /locus_tag="O2701_002224"
     CDS             7950..8765
                     /gene="phnE"
                     /locus_tag="O2701_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCZ4294099.1"
                     /translation="MTQEIAKYNVHTKAHKRKLIKRWLIAIVVLAIIIWAFAGVPSLE
                     LKSKSLEILKSIFSGLFHPDISYIYIPDGEDLLRGLLETFAIAVVGTFIAAIICIPLA
                     FLGANNMVKLRPVSGVSKFILSVIRVFPEIVMALIFIKAVGPGSFSGVLALGIHSVGM
                     LGKLLAEDIEGLDFSAVESLKASGANKIKTLVFAVIPQIMPAFLSLILYRFELNLRSA
                     SILGLIGAGGIGTPLIFAIQTRSWDRVGIILIGLVLMVAIVDLISGSIRKRIV"
     gene            complement(8896..10074)
                     /gene="deoB"
                     /locus_tag="O2701_002225"
     CDS             complement(8896..10074)
                     /gene="deoB"
                     /locus_tag="O2701_002225"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCZ4294100.1"
                     /translation="MTRPFNRVHLIVMDSVGIGEAPDAADFKDEGSHTLRHTLEGFDQ
                     TLPNLEKLGLGNIDKLPVVNAVEQPEAYYTKLSEASVGKDTMTGHWEIMGLNIMQPFK
                     VYPNGFPEELIQQIEEMTGRKVVANKPASGTQIIDEWGEHQMKTGDLIVYTSADPVLQ
                     IAAHEDIIPLEELYDICEKVRELTKDPKYLIGRIIARPYVGEPGNFTRTSNRHDYALK
                     PFGKTVLDHLKDGGYDVIAIGKINDIYDGEGVTEAVRTKSNMDGMDQLMKIVKKDFTG
                     ISFLNLVDFDALYGHRRDKPGYAQAIKDFDDRLPELFSNLKEDDLVIITADHGNDPTA
                     PGTDHTREYIPVIMYSPKFKGGHALESDTTFSSIGATIADNFNVTLPEFGKSYLKELK
                     "
     gene            complement(10102..>10404)
                     /gene="deoC"
                     /locus_tag="O2701_002226"
     CDS             complement(10102..>10404)
                     /gene="deoC"
                     /locus_tag="O2701_002226"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCZ4294101.1"
                     /translation="VKVIIETVLLDHDEIVKASELTKTAGADFVKTSTGFAGGGATAE
                     DVKLMKDTVGADVEVKASGGVRNLEDFNKMVEAGATRIGASAGVQIMQGLEADSDY"
BASE COUNT         3386 a         1908 c         1599 g         3511 t
ORIGIN      
        1 ttacaagcgc attttcgttc agtcaattac tgccaatata acttcgtaga tcatagaaga
       61 ttgatttatt tcccagccta ttctttttat aatatttagc aaaggtaata atgatattaa
      121 taatgtacaa aaaatataaa tcaaatcgac atccttataa aacatcagaa ccactaaaaa
      181 caaaaaagca caaaataaaa ttaaatttaa aataaacgac cacttttcaa aaaaatctct
      241 tttcatattt ccacccctaa ttttaataag cattatttta tattctcttt taagtttatt
      301 attcaaaagg aaaacagaaa tatctttcaa tattattata aacatttcaa ctacttttaa
      361 aaaccaacaa aaaaatactt attttaagta gatgagcata agtgaacata gttctttagt
      421 tataataatt aattcaacca aaagtcgatt tgtttttgca attggttttc atttcctctt
      481 aaagatattt tcattaaatc tgtcaaatca atagacgcta tatttttcaa cttatctcta
      541 tatttatttt tagtacgtct ttctaaattt ccccattcct cttcttcgtg agttaataaa
      601 tgaagcattg ctcgttcttg tatattttca atcattttta aattcggttt taaaatatgc
      661 aaatcatcaa aacaatcttt ccaacaatca accatatctc gttttaattc aatttccaca
      721 cgccatagaa atgttgaatc aatttcaaca tctgcattat ctttacgttc ttgtttttat
      781 tataaatccg aataaaccta tcactattac gcacaccaaa atattttgtt tctggtttta
      841 cattacgtcc ataaaatata gttttcttta ccgacttatc tgacaatgca taatagtcat
      901 ttaaatcaaa ttcaaaatca aaagccaaat ctaatctcgt aaaactaaca tcgtccaaat
      961 aactgatgat attttgtttt aaccaaagca cttcatcatg cgaaagctta ttaggattaa
     1021 attcaacgcg catactacgt ctattccaaa gagttgcttt tattttgtca tattcaatat
     1081 aaactttttc tttaagagct ttagctttaa agtttgtttg taaaatatcc caaagccgaa
     1141 tttcaggatt agtactcata aaatgtgaaa gtctctctgc gttagacatg ctaagattcc
     1201 caacaatcgt tatagcgtca aaagacaatt ttggaatagc tagtgacatc ctatgtcgat
     1261 ttaaccggct attaccggat attagagtat ccagttttac aaatggatga aacgaaattc
     1321 aaaacactaa aaaatatgtt ccactaacag caaaaaaata ccattatgtt cctactaaaa
     1381 aactaaaaat actggagaac aaatgtcagg atataactta ggatactatg taataaaaat
     1441 ttacaataaa aaaacaggaa aacaaatttc aagtaaaaga atacccatac aaagaggata
     1501 aaataaaaaa cctcgaactg aaatgatgat cttttcagct cgaggtttaa atattggtgc
     1561 cttatttata tagattcgtt atattatatt ctctattttc attaacataa tccttaaaga
     1621 gttttaaatt aatacctgct agatgattca aaaatgtttc atcaactttt aaataattca
     1681 ataatttttg tggtgtcagt aaatttctat caaaatacaa ctttaataaa ctattcattt
     1741 tgacaggacg tgacatttca atcacgtcgt ctaaagataa tactttctcg ctttaaacaa
     1801 aaacaaaaac ttacccgatt aaaatcaagt aagttttaca tatatccgtc acttttcaac
     1861 tttaaaatca acaacttgcg ggatgcgcat caaattatta tttgataaaa aatcaataag
     1921 taattgtgcg ccttcaactt gaatatcttt tacaactggc gcgtcgatat acatatcata
     1981 ctgaccaccg cctactgcac gataattatt tacacaaatt gtatatgtct gctttaaatc
     2041 aactgcgtga ccttgaatca tcatattgct cacacgttgt ccctttggtc ttccaacatg
     2101 aatggtataa cttacgccac catatatatc ataattaaag tgttgtggtt tgggttcaag
     2161 gaagtctgcg ctcacactaa cttcatcatt tttcacgtca aaatattctg ctgatcgttc
     2221 aatggcttct ttaagtttgg caccacttac agctaaaact ttaaatgtat ttggaaatgg
     2281 gtaattgtta ataacatctc gcatcgtcac gacttgcttg aaaccactag cagaatcaaa
     2341 caaagctgta caggcaacat ctgcgtcact tttttctaat aaagcgtaat tcataaaatt
     2401 tgtaaaagga tgcggtgcca cacgtgcctc aaatgcatga ttaatcgtca tatcatatgg
     2461 caatgtagta atttcgtaat ctaaccagtc ctctaactgc tttcgtaaat gttggtcatc
     2521 ttcatcaata gtaaatgtgg aatcatctat aacaggaagt aattcacatg attcaacgga
     2581 tagattttca tattcatcag tactcaagac tactctgcct acagttgtac ctctcgtacc
     2641 aggttgaatc acagccgttt gcttaaacct ttcagcaatt tgtcgatgtt ggtgacccgt
     2701 aataaagata tctatatctt tagaaaacgc ttctaacatg gcatatcctt cattttcacc
     2761 cgttaatact tcggtcggcg taccactttc taaatccttt tcaaatccac catggtaaca
     2821 aaccacaatg atatctgcat gtcgcttcat ttcaggtaag tattgttgaa gtatttcaaa
     2881 agcactatga aacgtaagtg actgaatatg ctctggttgt tcccaatggg gaataaattg
     2941 tgtcgttaaa cctatcacac caacagtttg atctccaacc tgaaaatact tcacaccgtt
     3001 atcagtcaat gtactatcat tttcatatat attagcgcac aaaactggat aattgagtct
     3061 gcgtaaagtg tcttttaagt atggtaatcc ataattaaat tcatgattac caagcgtacc
     3121 aaagtcgaat gccattcgat tataaaaatc aactaaaggc tggctactgc cgctatgcgc
     3181 gattaagtaa ttacaaaatg gtgacccttg caaaaaatca ccattatcta ttttaaaact
     3241 ttggtcatac tgccttctgt cttgttctat aacatgattc gctagtaaca atcccatagg
     3301 ttgatattga tttctactcg taaaatctgt tgggaaaata taaccatgta cgtcactcac
     3361 gacataaaat gctatgtttg acatcctcac tcactccttc aatcacaaac atctttctta
     3421 tttctattat atatttattt gaagtctgtt gtaatcaagg ttttgtcacc gagttttaaa
     3481 cgaatctttg aaccttccat actttcaagt actttagcat tgaccttaat tgtgacattt
     3541 ccgttttcat ctgctttaac tgttggcaaa gtactgtaac ctggtgggtt ataatcgtta
     3601 tctttacttg aaaattgtcc gatttgacgt ccgccttctt tgtaaatacc aactctaaga
     3661 ttttcaagtg tttgatttgc ttcaaatcct tttaaatgta ttgtcatttc aaatggctca
     3721 tttacagaaa cattttgcgg gatatcaaat gttacttttt cgttctgatt tggtggtgta
     3781 tgatcatctg gtgtgtttgg ctgaggatct gcgccttttt cgctgccata actacctgct
     3841 ttaaatgttg ttggatcata ccatttataa ccactcggcg gttgtgacca tggctctttt
     3901 tcaggctcag ttgaacgctc tggtcgttca aaatcaagca acttagtctt tgtatctaat
     3961 gttaggctac tcgccttaag tgatttccca tcattatctt tagacatcca agccgttata
     4021 ttatttaata gcttaccgtt gtcttgttct ttaaaaccat catatgtttt cttcttttct
     4081 ccattatctt ctcttacata tttgggcgaa ctatcttcca caagtgatga atcaccgata
     4141 aatgctgctt taccttttcc aactttagaa attgctacat aggggccttc tgctttaccg
     4201 cccccattat aaataccttg atctacagca tgtgaccatt tacttttcgc tggcaattgt
     4261 tctggtgtat acacaatacc ttttgctttc tctggattag taattgctaa tgtcgatccg
     4321 gcatgcatag agacagattt cacaccttca gtaataccga aactttcttt tgaagaaaca
     4381 atattgctcg tatttaaatc acctagtgca ttatatcgaa aacgtacgcc aaagtttgta
     4441 gataaccaat ctgaactttt cacaccttgc attgcagtag aacttttttc ttctgcattc
     4501 atacctttcg acatatcttc atatgctcca cgtcgataac cattcattgc ctccgatgaa
     4561 tcaatacgat ttaaatttcg gtcagcattg taatgatctg aaataaagac aacattgcca
     4621 ccttgtttca catatttaac aattgctgcc tgttctgatt ctttgaaagg aatgttagcc
     4681 tcaggaatta caaatatttt ggaacttttc aaacttgctt ctgttatgtt cgaatgacca
     4741 tcaatagctt taacgtcata accttgtttt tgtattgaat ccgcataatc tgaaaatgca
     4801 ccatcactaa cccaatctgc agcaccagct gtttgaccat gagaacgatc gaataatacc
     4861 gttcgctgtt gctttgtagg ttgcgattca tgcgttatag ctaaagattg cggtaaagca
     4921 cttaatgata ccgttgcaac aattgcagag acagttaatg acttatatat ttttttcatt
     4981 ttgtgaggct ccttttaaaa taaatttgtt cttgaattat aggataaaaa ttcgttgcat
     5041 atgagcaatt taacgaaaaa tttacaaaat cttatcaaac tcttaaagaa agttattaaa
     5101 attcattttt ataaaatact ttttaacatt taaatgtggt acgctataag tgtaatttca
     5161 ttgcatacat attacacgat taagaatgtg aaggggacag ttatcaaatg aaaaatttta
     5221 agtgtttatt tgtattaatg ttagcagtca ttgtttttgc agcagcatgt ggaaactcaa
     5281 gttctttaga taatcaaaag aacgctagta atgattcgga ttctaaatca ggaggataca
     5341 aacctaaaga attaaccgtt caatttgtac cttcgcaaaa tgctggaaca ttagaagcta
     5401 aagcaaaacc attagaaaaa ttactatcta aagaattagg gattccagtt aaagtgtctg
     5461 tatcaactaa ctacaataca attgttgaag ctatgaagtc taaaaaagtt gatgttggtt
     5521 tcttaccacc aacggcatac acattagcac atgatcaaaa agcagctgat ttattattac
     5581 aagcacaacg tttcggtgta aaagaagatg gttcagcaag taaagaactt gtagatagtt
     5641 ataaatcaga aattcttgtt aaaaaagact caaaaattaa aagcttgaaa gatttaaaag
     5701 gtaagaaaat tgccttacaa gatgtaacat caactgctgg atatacattc ccacttgcga
     5761 tgttaaaaaa cgaagcaggt attaatgcaa ctaaagatat gaaaattgtg aatgttaaag
     5821 gtcatgacca agcagttatc tcattattaa atggagatgt agatgctgcg gctgtattta
     5881 acgatgcacg taatactgtg aaaaaagacc aaccaaatgt atttaaagac acacgaattt
     5941 taaaattaac acaagctatt ccgaatgaca caatttctgt aagaccagat atggataaag
     6001 attttcaaga aaaattgaaa aaagctttta tagacattgc taaatcaaaa gaaggtcaca
     6061 aaattattag cgaagtttat tcacatgaag gatacacaga aacgaaagat tcaaatttcg
     6121 acattgtaag agagtacgaa aaattagtta aagatatgaa ataatcatta tttaacaaat
     6181 gaatcattag cgaatttggt attaaaagct ttcgttcaat agatatattc tagattaata
     6241 ttgaaaagct aggcgctaaa ctgaaacaga tatagaaagg tgtcgctgta catttgaaac
     6301 catttgtaca cagaaaccca atgtctatga tatttcagtt taccttggct tttctttatt
     6361 aaagaaaggt gtcaaacatg agtcaaatcg aatttaaaaa cgtcagtaaa gtctatccta
     6421 acggtcatgt aggcttgaaa aatattaact taaatattga aaaaggtgaa tttgcagtta
     6481 ttgtcggact atctggtgct gggaaatcca cgttattaag atctgtaaat cgtttgcatg
     6541 atatcacgtc aggtgaaatt ttcatccaag gtaaatcaat cactaaagcc catggtaaag
     6601 cattattaga aatgcgccga aatataggta tgattttcca acattttaat ttagttaaac
     6661 ggtcaagtgt attacgaaat gtactaagtg gacgtgtagg ttatcaccct acttggaaaa
     6721 tggtattagg tttattccca aaagaagaca aaattaaggc aatggatgca ctagaacgcg
     6781 tcaatatctt agataaatat aatcaacgct ctgatgaatt atcaggtggc caacaacaac
     6841 gtatatctat tgcacgtgcg ctatgccaag aatctgaaat tattcttgca gatgaaccag
     6901 ttgcttcatt agacccatta actacgaaac aggttatgga tgatttaaga aaaatcaacc
     6961 aagaattagg catcacaatt ttaattaatt tacattttgt tgacttggca aaagaatatg
     7021 gcacacgcat cattggttta cgtgatggtg aagttgtcta tgatggtcct gcatctgaag
     7081 caacagatga cgtatttagt gaaatatatg gacgtacaat taaagaagat gaaaagctag
     7141 gagtgaacta acatgccttt agaaatacct acaaagtatg actccctttt aaagaaaaag
     7201 gtttctttaa aaacgagttt taccttcatg ttaatcattg tgcttatcat ttggagcttc
     7261 atttatacgg gatttaattt tggagattta atgataggaa tacctcaaat aggtgatcta
     7321 ttcaaacaaa tgattccacc tgatttcgag tatttacaac aaattacaac gccaatgtta
     7381 gataccattc gaatggctat cgtaagtaca gtattaggta gcatcgtttc aataccaatt
     7441 gcgttattat gtgctagcaa tatcgttcat caaaagtgga tttcaatacc ctcgcgcttt
     7501 attttaaata tagttcgtac tattccagat ttgttattag cagcaatctt tgtggctgta
     7561 tttggaatcg gtcaaattcc agggatatta gcactgttta ttttaactat ctgtattatt
     7621 ggaaaattat tatatgaatc attggaaacg atagatccag gtccaatgga agcaatgacg
     7681 gctgttggcg ctaataaaat aaaatggatt gttttcggtg ttgtaccaca agccatatcg
     7741 tcatttatgt catacgtatt atatgcattt gaagtaaata tacgtgcttc agctgtgctt
     7801 ggattagtcg gcgctggcgg tattggattg ttttatgatc aaacacttgg tttatttcaa
     7861 tatccaaaaa cagcaacgat tattttattt actttagtta tcgtcgtcgt cattgattac
     7921 atcagtacga aagtgagggc acatctcgca tgacacagga aatagcaaaa tataatgttc
     7981 acacaaaagc acacaaacga aaattgatta aaagatggct tattgcaatt gtcgtcttag
     8041 ctattatcat ctgggcattt gcaggtgtac caagtttaga acttaaaagt aaatcattag
     8101 aaatcttaaa atccatattc agcggattat tccatcctga tatcagctat atctatatac
     8161 cagatggcga agacttatta cgtggtttac ttgaaacctt tgcgatagcc gttgtaggta
     8221 ctttcatcgc cgcaattatc tgtattccat tagcatttct aggtgcaaat aatatggtaa
     8281 agctacgccc agtttcaggt gttagcaaat ttattttaag tgttatacgt gtcttcccag
     8341 aaattgtaat ggcacttata tttatcaaag ctgttggccc aggttcattt tcaggtgtat
     8401 tagctttagg tatccattcc gtaggtatgc ttgggaaact tttagctgaa gatattgaag
     8461 gtctagattt cagtgctgta gaatcattaa aggccagtgg tgcgaataag attaaaacac
     8521 tcgtatttgc agtcatacca caaattatgc ctgcctttct atcactcata ctttatcgct
     8581 ttgaactaaa cttacgttca gcttctatac tggggctaat tggggctggt ggtatcggga
     8641 caccactcat atttgccatt caaacacgtt cttgggaccg tgtaggtatt atattaatcg
     8701 gtttagtact aatggtcgca attgtcgatt taatttccgg ttcaatccga aaacgtattg
     8761 tttaacatta aatcaggata ctcctaaata agaagtccta ccgtcttacg tttctctatt
     8821 ataataaaaa cagcagtgaa gaaaactatt gttatagtta acttcactgc tgtttttata
     8881 atatctaaat ttattctatt tcaattcctt taaataactt ttaccgaact ctggtaatgt
     8941 tacgttgaaa ttatctgcta tagttgcacc gatagaactg aatgtagtat cactttctag
     9001 tgcatgacca cctttaaatt tcggactgta cataattact gggatatatt ctctcgtatg
     9061 gtccgtacct ggcgctgtcg ggtcattacc atggtctgct gtaataatta ctaaatcgtc
     9121 ttcttttaag ttgctaaaca gttctggcaa gcgatcatcg aaatctttaa ttgcttgtgc
     9181 ataacctggt ttatcacgac gatgaccgta taatgcatca aagtctacta agtttaagaa
     9241 gctaatacct gtgaaatctt tcttaacaat tttcatcaat tgatccatac cgtccatgtt
     9301 actcttcgta cgaaccgctt ctgttacacc ttcaccatca taaatgtcat taattttacc
     9361 gatggcaata acatcataac caccgtcttt caaatgatct aagacagttt taccaaaagg
     9421 ttttaacgca tagtcatgtc gattagatgt acgtgtaaag tttcctggtt caccaacata
     9481 tggacgtgcg ataatacgac caattaaata tttagggtct tttgtcaact cacgaacctt
     9541 ttcacaaata tcatataact cttctaatgg gataatgtct tcatgtgcag caatttgcaa
     9601 tactgggtct gcacttgtat aaacaattaa gtcaccagtt ttcatttggt gctcgcccca
     9661 ctcatcgata atttgcgtac ccgatgccgg tttgttagca acaactttac gacctgtcat
     9721 ttcttcaatt tgttgaatta actcttcagg gaatccatta gggtatactt taaaaggttg
     9781 cataatattt aatcccataa tttcccagtg accagtcatt gtatctttac caactgaagc
     9841 ttcactcaat ttagtatagt atgcttctgg ttgttcaact gcatttacta ctggtaattt
     9901 atcgatgttc cctagaccta acttttcaag gtttggtaaa gtttgatcga aaccttctaa
     9961 ggtatgtctt aaagtatgtg aaccttcatc tttaaaatca gctgcgtctg gcgcttcacc
    10021 aatacctact gaatccatta cgattaaatg tacacgatta aatggtcttg tcatagctat
    10081 cactcccaaa atttatatat attagtaatc tgaatctgct tctaaacctt gcataatttg
    10141 aacacctgcg ctcgcaccaa tacgtgtcgc acctgcttca accattttat tgaaatcttc
    10201 taaattacgt acgccacctg atgcttttac ttctacatca gcacctactg tatctttcat
    10261 taatttaacg tcttctgcag tcgcaccgcc acctgcaaaa cctgttgaag ttttaacgaa
    10321 gtccgcacca gccgtttttg ttaattcact cgcttttaca atttcgtcat ggtccaacaa
    10381 taccgtctca ataatcactt ttac
//
LOCUS       DALAGD010000097        10199 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.098, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000097 DALAGD010000000
VERSION     DALAGD010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10199
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.098"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            136..1326
                     /locus_tag="O2701_002227"
     CDS             136..1326
                     /locus_tag="O2701_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HCZ4294102.1"
                     /translation="MTKSEKIIELTNHYGAHNYLPLPIVISEAEGVWVKDPEGNKYMD
                     MLSAYSAVNQGHRHPKIIQALKDQADKVTLVSRAFHSDNLGEWYEKICKLAGKDKALP
                     MNTGAEAVETALKAARRWAYDVKGIEPNKAEIIAFNGNFHGRTMAPVSLSSEAEYQRG
                     YGPLLDGFRKVDFGDVDALKAAINENTAAVLVEPIQGEAGINIPPEGYLKAIRELCDE
                     HNVLFIADEIQAGLGRSGKLFATDWDNVKPDVYILGKALGGGVFPISVVLADKEVLDV
                     FTPGSHGSTFGGNPLACAASIAALDVIVDEDLPGRSLELGDYFKEQLKQIDHPSIKEV
                     RGRGLFIGVELNESARPYCEALKEEGLLCKETHDTVIRFAPPLIITKEELDLALEKIR
                     HVFQ"
     gene            1435..2679
                     /locus_tag="O2701_002228"
     CDS             1435..2679
                     /locus_tag="O2701_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HCZ4294103.1"
                     /translation="MTENNNLVTSTQGIIKEALHKLGFDEGMYDLIKEPLRMLQVRIP
                     VRMDDGTVKTFTGYRAQHNDAVGPTKGGVRFHPDVDEEEVKALSMWMTLKCGIVNLPY
                     GGGKGGIVCDPRQMSIHEVERLSRGYVRAISQFVGPNKDIPAPDVFTNSQIMAWMMDE
                     YSALDKFNSPGFITGKPIVLGGSHGRDRSTALGVVIAIEQAAKRRNMQIEGAKVVIQG
                     FGNAGSFLAKFLYDLGAKIVGISDAYGALHDPNGLDIDYLLDRRDSFGTVTNLFEETI
                     SNKELFELDCDILVPAAISNQITEDNAHDIKASIVVEAANGPTTPEATRILTERGILL
                     VPDVLASAGGVTVSYFEWVQNNQGYYWSEEEVDEKLREKLEAAFDTIYELSQNRKIDM
                     RLAAYIIGIKRTAEAARYRGWA"
     gene            complement(3182..4111)
                     /locus_tag="O2701_002229"
     CDS             complement(3182..4111)
                     /locus_tag="O2701_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCZ4294104.1"
                     /translation="MTNSSKSFTKFMAASAVFTMGFLSVPTAGAEQTNQIANKPQAIQ
                     WHTNLTNERFTTIAHRGASGYAPEHTFQAYDKSHNELKASYIEIDLQRTKDGHLVAMH
                     DETVNRTTNGHGKVEDYTLDELKQLDAGSWFNKKYPKYARASYKNAKVPTLDEILERY
                     GPNANYYIETKSPDVYPGMEEQLLASLKKHHLLNNNKLKNGHVMIQSFSDESLKKIHR
                     QNKHVPLVKLVDKGELQQFNDQRLKEIRSYAIGLGPDYTDLTEQNTHHLKDLGFIVHP
                     YTVNEKADMLRLNKYGVDGVFTNFADKYKEVIK"
     gene            complement(4353..5732)
                     /gene="argH"
                     /locus_tag="O2701_002230"
     CDS             complement(4353..5732)
                     /gene="argH"
                     /locus_tag="O2701_002230"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCZ4294105.1"
                     /translation="MSNKAWGGRFEVQPEEWVDDFNASITFDQTLIDQDIEGSIAHAT
                     MLANQGIISQQDSEQIIQGLKSIQHDYHQDQIQFSASLEDIHLNIEHELIKRIGDAGG
                     KLHTGRSRNDQVATDMHLYTKKQVQDIIALIKSLQSVIVDIASNNVDTIMPSYTHLQR
                     AQPISFAHHIMTYFWMLQRDQQRFEDSLKRIDINPLGAAALSGTTYPIDRHETTALLN
                     FGSLYENSLDAVSDRDYIIETLHNISLTMVHLSRFAEEIIFWSTDEAKFITLSDAFST
                     GSSIMPQKKNPDMAELIRGKVGRTTGHLMSMLMTLKGLPLAYNKDMQEDKEGLFDAVH
                     TIKGSLRIFEGMIQTMTINKERLNQTVKEDFSNATELADYLVTKNIPFRTAHEIVGKI
                     VLECIQQGHYLLDVPLATYQQHHSSIDADIYDYLQPENCLKRRQSYGSTGQSSVKQQL
                     DVAKQLLSQ"
     gene            complement(5722..6927)
                     /locus_tag="O2701_002231"
     CDS             complement(5722..6927)
                     /locus_tag="O2701_002231"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCZ4294106.1"
                     /translation="MKEKIVLAYSGGLDTSVAVQWLIDKGYDVVACCLDVGEGKDLDI
                     VYKKALDMGAVECHIIDATKEFSDEYVSYAIKGNLMYENAYPLVSALSRPLIAKKLVE
                     IAEKTNSVGIAHGCTGKGNDQVRFEVAIKALNPSLKAFAPVREWAWSREEEIDYAIKH
                     NIPVSINHDSPYSIDQNLWGRANECGILEDPYAAPPEDAFDLTNALEETPDTADEIIL
                     TFDKGIPVQIDGKTYELDDLILTLNALAGKHGIGRIDHVENRLVGIKSREIYEAPAAE
                     VILKAHKALETITLTKDVAHFKPIIEKQFAEQLYNGLWFSPLTDSLKLFIDSTQQYVS
                     GDVRIKLFKGNAIVNGRKSPYTLYDEKLATYTKEDAFNQDAAVGFIDIYGLPTQVNAM
                     LHGGYSNEQ"
     gene            7278..8609
                     /locus_tag="O2701_002232"
     CDS             7278..8609
                     /locus_tag="O2701_002232"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCZ4294107.1"
                     /translation="MTHIQLDFSKTLEFFGEHELKQQQEIVKSIHKTIHEGTGAGSDF
                     LGWVDLPVDYDKEEFSRIVEASKRIKENSDVLVVIGIGGSYLGARAAIEMLTSSFRNS
                     NEYPEIVFVGNHLSSTYTKELVDYLADKDFSVNVISKSGTTTEPAVAFRLFKQLVEER
                     YGKEEAQKRIFATTDKEKGALKQLATNEGYETFIVPDDVGGRYSVLTAVGLLPIATAG
                     INIEAMMIGAAKAREELSSDKLEENIAYQYATIRNILYAKGYTTEMLINYEPSMQYFN
                     EWWKQLFGESEGKDFKGIYPSSANYTTDLHSLGQYVQEGRRFLFETVVKVNHPKYDIT
                     IEKDSDDLDGLNYLAGKTIDEVNTKAFEGTLLAHTDGGVPNMVVNIPQLDEETFGYVV
                     YFFELACAMSGYQLGVNPFNQPGVEAYKQNMFALLGKPGFEDLKKELEERL"
     gene            8934..9509
                     /locus_tag="O2701_002233"
     CDS             8934..9509
                     /locus_tag="O2701_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCZ4294108.1"
                     /translation="MSFHQVEEWFEIFRQFGYLPGFILLYIRAIIPVFPLALYILINI
                     QAYGPILGILISWLGLISGTFTVYLICKRLVNTERMQRIKQRTAVQRLISFIDRQGLI
                     PLFILLCFPFTPNTLINFVASLSHIRPKYYFIVLASSKLVSTIILGYLGKEITTILTH
                     PLRGILMLVVLVVFWIVGKKLEQHFMGSKKE"
     gene            9514..10038
                     /gene="lepB"
                     /locus_tag="O2701_002234"
     CDS             9514..10038
                     /gene="lepB"
                     /locus_tag="O2701_002234"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCZ4294109.1"
                     /translation="MKKVVKYLISLILAIIIVLFVQTFVIVGHVIPNNDMSPTLNKGD
                     RVIVNKIKVTFNQLNNGDIITYRRGNEIYTSRIIAKPGQSMAFRQGQLYRDDRPVDAS
                     YAKNRKIKDFSLRNFKELDGDIIPPNNFVVLNDQDNNKHDSRQFGLIDKKDIIGNVSL
                     RYYPFSKWTVQFKS"
     gene            10054..>10199
                     /locus_tag="O2701_002235"
     CDS             10054..>10199
                     /locus_tag="O2701_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HCZ4294110.1"
                     /translation="MKKEILEWIISIAVAFVILFIVGKFIVTPYTIKGESMDPTLKDG
                     ERVAV"
BASE COUNT         3368 a         1579 c         1862 g         3390 t
ORIGIN      
        1 aaatagacaa atttaatgaa acttagtgat tatttcatct tttttggtgg aaaagtatct
       61 gctttgaaaa ctaatgtaat tatttggata atgtttcatg tataatagtt ataaatgatg
      121 aaaaggggag atatgatgac taaatctgaa aaaattattg agttaacaaa tcattacgga
      181 gcacataatt atttaccatt gccaattgtc atttcagaag ctgaaggggt atgggttaaa
      241 gatcctgaag gcaataaata tatggatatg ttatctgcat attccgctgt taaccaaggt
      301 catagacatc cgaaaattat tcaagcatta aaagatcaag ctgataaagt gactttagtt
      361 tcacgtgctt ttcatagtga taacttaggt gaatggtacg aaaaaatttg taaactggca
      421 ggtaaagata aagctttacc aatgaataca ggtgctgaag cagtagaaac agctttgaaa
      481 gcagcacgac gctgggcata cgatgttaaa ggaattgagc caaataaagc agaaatcatt
      541 gcatttaatg gtaacttcca tggtcgaaca atggcgccag tttcattatc ttcagaagca
      601 gaataccaac gtggttatgg tccgttatta gatggattta gaaaagttga ttttggagat
      661 gtagatgcat tgaaagctgc aattaatgaa aatactgcag cagttttagt agaaccaatt
      721 caaggtgaag cgggtataaa tataccgcca gaaggatatt tgaaagcaat tagagaatta
      781 tgtgatgaac ataatgtctt atttattgct gacgaaatcc aagcaggatt aggtcgttcg
      841 ggtaaattat ttgctacgga ttgggataat gtaaaacctg atgtctatat tttaggtaaa
      901 gcactaggtg gtggagtctt cccaatttct gttgtattag cagataaaga agtattagat
      961 gtctttacac ctggctcaca tggttcaaca tttggtggta atccacttgc ttgtgctgca
     1021 tcaattgctg cattagatgt tatcgttgat gaggatttac caggccgctc tttagaatta
     1081 ggagattatt ttaaagaaca attaaagcaa attgatcatc catcaattaa agaagtccgt
     1141 ggacgtggtt tgtttatagg tgtggaactt aatgaaagtg ctagaccata ttgtgaagct
     1201 ttgaaagaag aaggcttatt atgtaaagaa acgcatgata ctgtcattcg ttttgcacca
     1261 ccattaatta ttactaaaga agaattggac cttgcacttg aaaaaataag acatgtattt
     1321 caataatgtc aaaaagttgt aacaatcagt ttgtaagggc tttaaaaagg aatacgttat
     1381 gttacaatat gtttgaaagc gaaatcatta ggtaaagaag aggcgaaaag gatcatgact
     1441 gagaacaata atttagtaac ttctactcaa ggaattatta aagaagcatt gcataaattg
     1501 ggatttgacg aaggaatgta cgatttaatt aaagaacctt taagaatgtt acaagtgcgt
     1561 atccctgtac gaatggatga tggcacagta aaaacattca caggttaccg tgcgcaacat
     1621 aatgatgctg ttggaccaac aaaagggggc gtgcgtttcc acccagatgt tgatgaagaa
     1681 gaagtaaaag cattatcaat gtggatgact ttgaaatgtg gcattgtaaa cttaccatac
     1741 ggtggtggta agggtggtat cgtttgtgat ccacgtcaaa tgagcattca tgaagttgaa
     1801 cgtttatcac gcggatatgt aagagcaatt tcacaattcg taggtccgaa caaagatatt
     1861 ccagcaccag atgtatttac aaactcacaa attatggctt ggatgatgga tgaatatagt
     1921 gcattagata aatttaattc accaggtttc atcacaggta aaccaattgt attgggtggt
     1981 tctcatggac gcgacagatc aactgcacta ggtgtagtta ttgcaattga acaagctgca
     2041 aaacgtcgta atatgcaaat tgaaggtgcc aaggttgtta ttcaaggttt cggtaatgcc
     2101 ggaagtttct tagctaaatt cttatatgat ttaggtgcaa aaattgtagg tatctctgat
     2161 gcttacggtg cattacacga tccaaatggc ttagatatag attatttatt agaccgtcgt
     2221 gatagttttg gtacggtaac aaatttattt gaagaaacaa tctcaaataa agaattgttt
     2281 gaattagatt gtgacatttt agtaccagcg gctatttcaa accaaattac agaagacaat
     2341 gcacatgata ttaaagctag tatcgttgtt gaagctgcta atggacctac aacaccagaa
     2401 gcaacacgta ttttaactga acgtggtata ttattagttc cagacgtatt agcaagtgct
     2461 ggtggtgtaa cggtttctta cttcgaatgg gtacaaaata atcaaggtta ttattggtct
     2521 gaagaagaag ttgatgaaaa gctacgtgaa aaattagaag cggcatttga tacgatttac
     2581 gaattgtctc aaaaccgaaa aatagatatg agacttgcag catatatcat aggtattaaa
     2641 cgtacagcag aagcagctag atatcgtggt tgggcataat taattatcat atgtgattta
     2701 acgagcttgg gacagaaaac aaagccctaa gctcgttaat tttattttag cagtagttga
     2761 ctgtaaaaca atgcccgtgt aacacgctct tttcaaaaat agtcggggcc ccaacacaga
     2821 ggctggtgga aagtcagctt acaatagtgt gcaagttggc ggggccccaa cacagaggct
     2881 ggcggaaagt cagcttacaa tagtgtgcaa gttggggtgg agccccgaca cagagaaatt
     2941 agctcctcaa tttctacaga caatgcaagt tggcggggcc ccaacacaga agctggcgaa
     3001 aagtcagctt acaatagtgt gcaagttggg gtgggacgac gaaataaatt ttgcgaaaat
     3061 atcatttctg tcccactccc aaaaagacag cagtaggata atttcaattt ggaaatatct
     3121 tactgctgtt ttttatagta atagctatat ttttgtattt atgttttcta gtttaacatt
     3181 actacttaat gacttcttta tatttatcag cgaaatttgt aaagacacca tcaacgccat
     3241 atttatttaa tcgtaacata tcagcttttt cattcactgt ataaggatgt actataaatc
     3301 ctaagtcttt taaatgatgg gtattttgtt cagttaaatc tgtataatca ggacctaatc
     3361 caatcgcata agagcgtatc tcttttaagc gttggtcgtt aaattgttgt agttcacctt
     3421 tatcaactaa ttttactaat ggcacatgct tattttgacg atgaattttc tttaaacttt
     3481 cgtcagaaaa tgattgaatc attacatgtc cattttttaa tttattgtta tttaaaaggt
     3541 gatgcttttt caatgaagct aataattgtt cttccattcc tgggtataca tcaggtgact
     3601 ttgtttcaat ataatagttt gcattcgggc cataacgttc taaaatttca tctaaagtgg
     3661 gtactttagc atttttataa cttgctcttg cgtattttgg atatttttta ttaaaccaac
     3721 ttcctgcatc taactgtttt aattcatcaa gggtataatc ctcaacttta ccgtgtccat
     3781 ttgttgtacg gttaacagtt tcatcatgca tagcaactaa atggccatct ttggtacgtt
     3841 gtaaatcaat ttcgatataa gatgctttta actcattatg actcttatca tatgcttgaa
     3901 acgtatgctc gggtgcatag ccacttgcgc cacgatgtgc gatagtagtg aatcgctcat
     3961 tcgttaaatt tgtatgccat tgaatagcct gaggtttatt tgcaatttga tttgtttgtt
     4021 cagcgccagc agtaggtact gataaaaatc ccatagtaaa aacagcagaa gcagccataa
     4081 atttagtgaa gcttttcgaa gagttagtca tagtctttat cctttcatct ctttatttgt
     4141 agtctaacaa ttttaaaata ctacaaaaac ctgcttgtga aaacgcttta cacatttctt
     4201 ttacaattta taacattttt gtaatataaa tttaaaagaa ttatcaaaca ttttaggaat
     4261 cttaaaaatg tgatgttcaa caagacgaag agagcattaa aatacccaca atgtaatttt
     4321 aatagacatt gtgggtaggt agattaacgt atttattgtg atagtaattg tttagcaaca
     4381 tcaagttgtt gtttgaccga tgattgacct gttgaaccgt aactttgacg tcgttttaaa
     4441 caattttcag gctgcaaata atcgtaaata tcggcatcaa tactagaatg atgttgttga
     4501 tatgttgcta aaggaacatc taataaataa tgaccttgtt gtatacattc taagacgatt
     4561 tttcctacaa tttcatgtgc agttctaaat ggaatatttt tagttactaa ataatctgct
     4621 agttccgttg catttgaaaa atcttcttta acagtttgat tgagtcgttc tttattaatt
     4681 gtcatcgttt gaatcatacc ttcgaaaata cgtaaagaac ctttaattgt atggacagca
     4741 tcgaataaac cttctttatc ttcctgcata tctttgttat atgctagagg taatcctttt
     4801 aaagtcataa gcatgctcat taaatgacca gtcgttcgac caactttacc tctaattaat
     4861 tctgccatat caggattttt cttttgtggc ataatagatg agccagttga aaatgcatct
     4921 gataatgtaa tgaatttagc ttcgtctgtg gaccagaaaa taatttcctc tgcaaagcgt
     4981 gataagtgaa ccatcgttaa agaaatatta tgcaatgttt caataatata gtctctgtca
     5041 ctaacagcat ctaggctatt ctcatagaga ctgccaaagt tcaacaatgc tgttgtctcg
     5101 tgtctatcga tagggtatgt ggtaccactt aaggctgctg cacctaaagg attaatatcg
     5161 attcgtttta aactatcttc aaatcgttgt tggtctcgtt gtaacatcca aaaataagtc
     5221 ataatatgat gtgcaaatga aattggctgt gcacgctgta aatgagtata actaggcata
     5281 attgtatcaa cattattgga agcgatgtct acaattacac tttgtaacga cttaattaat
     5341 gcgatgatat cttgcacttg tttcttagtg tacaagtgca tgtctgttgc aacttgatcg
     5401 tttctactgc gtccagtatg caacttacca ccagcatcac cgatacgttt aattaattca
     5461 tgttcaatat ttaaatgaat atcttctaat gatgcactaa attgaatttg atcttgatga
     5521 taatcatgtt gaatagattt tagtccttgt ataatttgtt cgctgtcttg ttgactaata
     5581 atgccttgat tcgcaagcat agttgcatgt gcaatgctgc cttcgatatc ttgatctatg
     5641 agcgtttgat caaaagtaat ggatgcgtta aagtcgtcaa cccactcttc aggttgtact
     5701 tcaaatctac cgccccaagc tttattgctc attgctatag cctccatgca acatcgcatt
     5761 tacttgagta ggtaaaccat agatatcgat aaagccaaca gcagcgtctt gattaaatgc
     5821 atcttctttt gtataagttg ctaatttttc atcatataat gtgtaaggtg attttctacc
     5881 attcacgatg gcattacctt tgaataattt aattctgaca tcaccactta cgtattgctg
     5941 agtactatca ataaataatt tcaagctatc agttaaaggt gagaaccaaa gtccattgta
     6001 tagttgttca gcaaattgct tctcaatgat tggtttaaag tgtgcgacat ctttcgttaa
     6061 cgtaatcgtt tctaatgctt tatgcgcttt taaaataact tctgcagcag gtgcctcata
     6121 aatttctctt gatttgatac ctacaagtct attttctaca tggtcaattc ttccgatacc
     6181 atgcttacca gctaatgcat tcaacgttaa aattaaatcg tctaattcat atgttttgcc
     6241 atcaatttga actgggatgc ctttatcaaa cgttaaaatg atttcatcag cagtatctgg
     6301 tgtttcttct aaagcatttg ttagatcgaa cgcatcctct ggtggcgcag cataaggatc
     6361 ttctaaaata ccacattcat tcgctctgcc ccatagattt tgatcgatag aataaggtga
     6421 atcatggttg attgatacag ggatattatg tttaattgca taatcgattt cttcttcacg
     6481 actccatgcc cactcacgta caggtgcgaa tgctttcaat gatgggttta atgctttaat
     6541 ggcaacttcg aaacgtactt ggtcattccc tttaccagta caaccatgtg caatacctac
     6601 tgaatttgtt ttctcagcaa tctctactaa ttttttagcg attaatggtc ttgataaagc
     6661 tgaaactaat ggatatgcat tttcatacat taaatttcct ttgattgcat aacttacata
     6721 ctcatcacta aattcttttg ttgcatcaat aatatgacat tcaactgctc ccatatctaa
     6781 agctttttta taaacgatgt ctaaatcttt accttcacca acatctaggc aacaagctac
     6841 aacgtcgtat cctttgtcga taagccattg aacggccaca cttgtatcta gtcctcctga
     6901 atatgctaaa acaattttct ctttcataaa attcacctca ttgtagttat ctgaatattt
     6961 cgtatgaaca catgatagca aacttttaat gaaaataaaa ccattatttt acatatttat
     7021 tcataaaatg cgatagagat agttagagtt gtaagtgtaa atgcttattt atcaatgttt
     7081 aaaagtattt taaaaaacat gcaaattgta taaaaaatgt ttgagtgttt gacgccagtt
     7141 tgaaatgttt gttgaaaaat aggtgtgaaa cgtcacataa atatcaattt tattgattaa
     7201 gtacaaactt gtgaattgaa tcgcaaacgt aaacttagta aaattaagat attaaatgaa
     7261 acaggaggct tttatagatg actcatattc aattagattt tagtaaaacg ttagaatttt
     7321 tcggtgaaca cgaattaaaa caacaacaag aaattgttaa atcaattcac aaaacaattc
     7381 atgaaggtac tggtgcaggt agtgacttct taggctgggt tgatttacca gttgattacg
     7441 acaaagaaga attttcaaga attgttgaag catcaaaacg cattaaagaa aattctgatg
     7501 ttttagtagt catcggtatt ggtggttctt acttaggtgc acgtgcagca atcgaaatgt
     7561 taacgtcatc atttagaaac agcaatgaat accctgaaat tgtatttgtt ggtaatcact
     7621 tatcatcaac atatacgaaa gagttagttg attatttagc agacaaagat ttctctgtaa
     7681 acgttatttc taaatctggt acaactacag aaccagcagt tgcatttaga ttgttcaaac
     7741 aattagttga agaaagatac ggtaaagaag aagcacaaaa acgtatattt gcaacaacgg
     7801 ataaagaaaa aggtgcttta aaacagttgg ctacaaacga aggttatgaa acgtttatcg
     7861 tacctgatga tgtaggtgga agatattctg ttttaacagc agtaggatta ttaccaattg
     7921 caacagctgg aattaacatc gaagctatga tgattggtgc tgcaaaagca cgtgaagaat
     7981 tatcttcaga taaattagaa gaaaacattg cataccaata tgcgacaatt cgaaacattt
     8041 tatatgcaaa aggttataca acagaaatgt tgattaacta tgaaccatct atgcaatact
     8101 ttaatgaatg gtggaaacaa ttatttggtg aatcagaagg taaagacttc aaaggtatct
     8161 atccttcaag tgccaactac acaactgatt tacattcttt aggtcaatat gtacaagaag
     8221 gccgtcgctt cttattcgaa acagttgtaa aagtaaatca tcctaaatat gatattacta
     8281 ttgaaaaaga tagtgatgat ctagacggat taaattattt agctggtaaa acaatcgacg
     8341 aagttaacac aaaagcattc gaaggtacat tattagcgca tactgatggt ggtgttccta
     8401 acatggtagt gaacattcca caattagatg aagaaacttt cggttacgtc gtatacttct
     8461 tcgaacttgc ttgtgcaatg agtggatacc aattaggcgt aaatccattt aaccaacctg
     8521 gtgtagaagc atataaacaa aacatgttcg cattattagg taaacctggt tttgaagact
     8581 tgaaaaaaga attagaagaa cgtttataaa atacattact tcaaagatta gtgaagtttg
     8641 aaaagataga actagacgtt aactatttaa agcatatttt cgaggttgtc attacaaatg
     8701 taaaaatgta atgacaacct cgtttttatt tatatgcaag aactaggtta ctagctaatg
     8761 tgacaagatg ttaagagaaa attaaagata aaataatatc tgccttacaa taatattgtt
     8821 atactactag agactgattt attagcatga ttacatgtta atgtttcttt acttagtaat
     8881 taactttata atgtaagaat aattatcttc aaccaaagaa agggattgat gatttgtcgt
     8941 ttcatcaagt agaagaatgg tttgagatat ttcgacagtt tggttattta cctggattta
     9001 tattgttata tattagagct ataattccag tatttccttt agcactctat attttaatta
     9061 acattcaagc ttatggacct attttaggta tattgattag ttggcttgga ttaatttctg
     9121 gaacatttac agtctatttg atctgtaaac gattggtgaa cactgagagg atgcagcgaa
     9181 ttaaacaacg tactgctgtt caacgcttga ttagttttat tgatcgccaa ggattaatcc
     9241 cattgtttat tttactttgt tttcctttta cgccaaatac attaataaat tttgtagcga
     9301 gtctatctca tattagacct aaatattatt tcattgtttt ggcatcatca aagttagttt
     9361 caacaattat tttaggttat ttaggtaagg aaattactac aattttaacg catcctttaa
     9421 gagggatatt aatgttagtt gtgttggttg tattttggat tgttggaaaa aagttagaac
     9481 agcattttat gggatcgaaa aaggagtgac atcgtgaaaa aagttgtaaa atatttgatt
     9541 tcattgatac ttgctattat cattgtactg ttcgtacaaa cttttgtaat agttggtcat
     9601 gtcattccga ataatgatat gtcaccaacc cttaacaaag gggatcgtgt tattgtaaat
     9661 aaaattaaag ttacatttaa tcaattgaat aatggtgata tcattacata taggcgtggt
     9721 aacgagatat atactagtcg aattattgcc aaacctggtc aatcaatggc gtttcgtcag
     9781 ggacaattat accgtgatga ccgaccggtt gacgcatctt atgccaagaa cagaaaaatt
     9841 aaagatttta gtttgcgcaa ttttaaagaa ttagatggtg atattattcc gccaaacaat
     9901 tttgttgtgc taaatgatca agataataac aagcacgatt caagacaatt tggtttaatc
     9961 gataaaaagg atattattgg taatgttagt ttacgatact atcctttttc aaaatggact
    10021 gttcagttca aatcttaaaa agaggtgtca aaattgaaaa aagaaatatt ggaatggatt
    10081 atttcaattg cagtcgcttt tgtcatttta tttatagtag gtaaatttat tgttacgcca
    10141 tatacaatta aaggtgaatc aatggatcca actttgaaag atggcgagcg agtagctgt
//
LOCUS       DALAGD010000098        10167 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.099, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000098 DALAGD010000000
VERSION     DALAGD010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10167
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.099"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            132..992
                     /locus_tag="O2701_002236"
     CDS             132..992
                     /locus_tag="O2701_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCZ4294111.1"
                     /translation="MLSVAIIGPGAVGTTIAYELQQSLPHTTLIGRHAKTITYYTVPH
                     APAQDIVVKGYEDVTNTFDVIIIAVKTHQLDAVIPHLTHLAHEDTLIILAQNGYGQLE
                     HISFKNVCQAVVYISGQKKGDVVTHFRDYQLRIQDNALTRQFRDLVQDSQIDIVLEAN
                     IQQAIWYKLLVNLGINSITALGRQTVAIMHNPEIRTLCRQLLLDGCRVAQAEGLNFSE
                     QTVDTIMTIYQGYPDEMGTSMYYDIAHQQPLEVEAIQGFIYRRAREHNLDTPYLDTIY
                     SFLRAYQQNM"
     gene            complement(1078..1782)
                     /gene="budA"
                     /locus_tag="O2701_002237"
     CDS             complement(1078..1782)
                     /gene="budA"
                     /locus_tag="O2701_002237"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCZ4294112.1"
                     /translation="MTNVLYQHGTLGTLMAGLLEGTATINELLEHGNLGIATLTGSDG
                     EVIFLDGKAYHANEHKEFIELKGDEKVPYASITNFKASKTFPLQQLSQDDVFAQIKNE
                     MLSENLFSAVKIYGTFKHMHVRMMPAQQPPYTRLIDSARRQPEEKRQDIRGAIVGFFT
                     PELFHGVGSAGFHIHFADDERAYGGHVLDFEVDDVVVEIQNFETFQQHFPVNNETFVK
                     AKIDYKDVAEEIREAE"
     gene            complement(2060..3019)
                     /locus_tag="O2701_002238"
     CDS             complement(2060..3019)
                     /locus_tag="O2701_002238"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCZ4294113.1"
                     /translation="MKTFGKKVVLIGDGSVGSSYAFAMVTQGVADEFVIIDIAKDKVK
                     ADVQDLNHGTVHSPSPVDVKAGEYEDCKDADLVVITAGAPQKPGETRLQLVEKNTKIM
                     KSIVKSVMDSGFDGYFLIAANPVDILTRFVKEYTGLPAERVIGSGTVLDSARLQYLIS
                     QELGVAPSSVDASIIGEHGDTELAVWSQANVAGISVYDTLKEQTGSEAKAEEIYVNTR
                     DAAYEIIQAKGSTYYGIALALMRISKAILNNENNVLNVSIQLDGQYGGHKGVYLGVPT
                     LVNQHGAVKIYEMPLSAEEQALFDKSVKTLEDTFDSIKYLLED"
     gene            3706..5154
                     /locus_tag="O2701_002239"
     CDS             3706..5154
                     /locus_tag="O2701_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCZ4294114.1"
                     /translation="MGSFFNKIARKEDPAIYQNKDGHLKRTLRVRDFLALGVGTIVST
                     SIFTLPGIVAAEHAGPAVALSFLLAAIVAGLVAFTYAEMAAAMPFAGSAYSWVNVLFG
                     EFFGWVAGWALLAEYFIAVAFVASGFSANLRGLVKPIGIELPAALSNPFGTNGGFIDI
                     IAAIVILLTALLLSRGMSEAARMENILVILKVLAIILFVIVGLTAINVSNYVPFIPEH
                     KVTATGDFGGWQGIYAGVSMIFLAYIGFDSIAANSAEALDPQKTMPRGILGSLSVAIV
                     LFIAVALVLVGMFHYSQYANNAEPVGWALRQSGHGVVAAIVQAISVIGMFTALIGMML
                     AGSRLLYSFGRDGLLPSWLSHLNDKHLPNRALVILTIIGVLIGSMFPFAFLAQLISAG
                     TLVAFMFVSLAMYRLRKREGKDLPIPAFKLPLYPVLPAITFVLVLLVFWGLGFEAKLY
                     TLIWFIVGIILYLSYGLRHSKKNDVAEYHPPK"
     gene            complement(5235..6572)
                     /locus_tag="O2701_002240"
     CDS             complement(5235..6572)
                     /locus_tag="O2701_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HCZ4294115.1"
                     /translation="MSKAHQLIQEDEHYFAKSGRIKYYPLVIDHGYGATLVDIEGKTY
                     IDLLSSASSQNVGHAPREVTEAIKAQVDKFIHYTPAYMYHEPLVRLAKKLCEIAPGDF
                     EKRVTFGLTGSDANDGIIKFARAYTGRPYIISFTNAYHGSTFGSLSMSAISLNMRKHY
                     GPLLNGFYHIPFPDKYRGMYEQPQANSVEEYLAPLKEMFAKYVPADEVACIVIETIQG
                     DGGLLEPVPGYFEALEKICREHGILIAVDDIQQGFGRTGTWSSVSHFNFTPDLITFGK
                     SLAGGMPMSAIVGRKEIMNCLEAPAHLFTTGANPVSCEAALATIQMIEDQSLLQASAE
                     KGEYVRKRMDQWVSKYNSVGDVRGKGLSIGIDIVSDKKLKTRDASAALKICNYCFEHG
                     VVIIAVAGNVLRFQPPLVITYEQLDTALNTIEDALTALEAGNLDQYDISGQGW"
     gene            6830..7246
                     /locus_tag="O2701_002241"
     CDS             6830..7246
                     /locus_tag="O2701_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294116.1"
                     /translation="MRLKVLFHFIAAIFISFMLLWMTMLFDLISNQSHLKALLLNLDF
                     LIPSDNTPYILEIICHLLIGSVIYFVFVLLFHTSKRLYYLCYIPLFFLFIALYPFLVF
                     IAQRPIFQFSVTELIGWIITHIFFMSLMALVIPRIK"
     gene            7369..8259
                     /locus_tag="O2701_002242"
     CDS             7369..8259
                     /locus_tag="O2701_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002478556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HCZ4294117.1"
                     /translation="MNKEQLEKMKNGKGFIAALDQSGGSTPKALKEYGVNEDQYSNED
                     EMFQLVHDMRTRVVTSPSFSPDKILGAILFEQTMDREVESKYTADYLADKGVVPFLKV
                     DKGLAEEQNGVQLMKPIDNLDNLLDRANERHIFGTKMRSNILELNEQGIKDVVEQQFE
                     VAKQIIAKGLVPIIEPEVNINAKDKAEIEKVLKAELKKGLDSLNADQLVMLKLTIPTE
                     PNLYKELAEHPNVVRVVVLSGGYSREKANELLKDNAELIASFSRALASDLRADQSKEE
                     FDKALGDAVESIYDASVNKN"
     gene            complement(8452..9948)
                     /gene="mqo"
                     /locus_tag="O2701_002243"
     CDS             complement(8452..9948)
                     /gene="mqo"
                     /locus_tag="O2701_002243"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCZ4294118.1"
                     /translation="MAKSNSKDIVLIGAGVLSTTFGSMLKEIEPDWNIHVYERLDRPA
                     IESSNERNNAGTGHAALCELNYTVLQPDGSIDIEKAKVINEEFEISKQFWGHLVKSGS
                     IENPREFINPLPHISYVRGKNNVKFLKDRYEAMKAFPMFDNIEYTEDIEVMKKWIPLM
                     MKGREDNPGIMAASKIDEGTDVNFGELTRKMAKSIEAHPNATVQFNHEVVDFEQLSNG
                     QWEVTVKNRLTGEKFKQVTDYVFIGAGGGAIPLLQKTGIPESKHLGGFPISGQFLACT
                     NPQVIEQHDAKVYGKEPPGTPPMTVPHLDTRYIDGQRTLLFGPFANVGPKFLKNGSNL
                     DLFKSVKTYNITTLLAAAVKNLPLIKYSFDQVIMTKEGCMNHLRTFYPEARNEDWQLY
                     TAGKRVQVIKDTPEHGKGFIQFGTEVVNSQDHTVIALLGESPGASTSVSVALEVLERN
                     FPEYKTEWAPKIKKMIPSYGESLIEDEKLMRKIRKQTSKDLELGYYEN"
BASE COUNT         3154 a         1867 c         1626 g         3520 t
ORIGIN      
        1 accatagaaa ttttagtttg cttttgtttc atatctatta attgacttac tgttttcaat
       61 gttattcaac ctctttttgc agtattatta gaaatagtat aatgaatctt ttatagaaag
      121 gaaagaagaa gatgttatca gttgcgatta tcggcccagg cgctgtcggt acaactattg
      181 cctatgaatt acaacaatca ttgcctcata caacgcttat cgggagacac gccaaaacaa
      241 taacatatta tactgtacca catgcacctg cacaagatat tgttgtgaaa ggttatgaag
      301 atgtcacaaa cacatttgat gttattatta ttgcagtcaa aacacatcaa cttgatgcag
      361 ttattcctca tttaactcat cttgcacatg aggacacgct catcatttta gcccaaaatg
      421 gttatggtca actcgaacat atttcattta aaaacgtatg ccaagcagtt gtctatataa
      481 gtggtcaaaa gaaaggcgat gttgttacgc acttcagaga ttatcaatta cgtatacaag
      541 ataatgcatt aactcgacaa tttagagatt tggtccaaga cagtcaaatc gacattgtat
      601 tagaagcaaa tattcaacaa gctatttggt ataaattgtt agttaacttg ggcattaatt
      661 ctatcacagc actcggtaga cagaccgttg caatcatgca taatcctgaa atacgtacac
      721 tgtgtagaca gctattacta gatggttgtc gtgttgcaca ggcagagggg ttaaactttt
      781 cagaacaaac tgtcgatact attatgacta tatatcaagg ttatcccgac gaaatgggaa
      841 cgagcatgta ttacgacatt gcgcatcagc aacctcttga agtcgaagcg attcaaggct
      901 ttatttatcg acgtgcacgt gaacacaatc ttgacacccc atatcttgat actatttata
      961 gctttttacg cgcgtatcaa caaaatatgt aagcacatat ataatataaa aagaccttaa
     1021 cgatgcattt agaaccacct gttcctaata tcgctaaggt cttttaatta tattagacta
     1081 ttcagcttct ctaatttctt ctgccacatc tttatagtct attttcgctt taacaaacgt
     1141 ctcgttattt accgggaaat gttgttggaa tgtttcaaag ttttgtatct caacgacaac
     1201 gtcatccact tcaaagtcaa gaacatgtcc accataagct ctttcatcat ccgcaaaatg
     1261 tatatgaaaa ccagcagacc ctacgccatg aaataattct ggtgtaaaaa atccaacaat
     1321 ggcaccacga atatcttgtc ttttttcctc aggttgtctg cgtgctgaat caatcaaacg
     1381 tgtatatggc ggttgctgag caggcatcat tcgtacatgc atatgtttaa atgtgccata
     1441 aattttaaca gccgaaaata aattctcact taacatttca tttttaattt gtgcaaatac
     1501 atcatcttgt gataattgtt gcaatggaaa tgtcttactc gctttaaaat tagtaatcga
     1561 tgcatacggt actttctcat cgccttttaa ttctataaat tctttatgct cgttagcatg
     1621 atatgccttt ccgtctaaaa atattacttc gccatcagac cctgttaacg ttgcaatccc
     1681 taaattccca tgttctaaca attcattaat tgtagctgtg ccttctagta agccagccat
     1741 taacgtacct aatgtaccat gttgatacaa gacattcgtc attccaaaac catctcccta
     1801 tagttattcc tcgttgtcat gctctacttc gtgattaatg cagaactact ttcctttata
     1861 ctcaatgacg cctcaacatt tttattcatg tcactaaatt ttcacgagtc ataatacttt
     1921 attcaattat tattttacgc aaaagtattg cctctatcaa tgcttgctaa aaaaatggac
     1981 ctatcagtgc cacgtgttac tgcaacacta atagatcctc ttaccattaa aatatttcta
     2041 attaatactt aaaataggct tagtcttcta ataaatattt aattgaatca aatgtatctt
     2101 ctaatgtttt aacagattta tcgaacaacg cttgttcttc ggcacttaat ggcatttcat
     2161 aaattttaac tgcgccatgt tggttaacta atgttggtac acctaggtaa acgcctttgt
     2221 gaccaccata ttgaccatct aattgtatag aaacatttaa gacattattt tcattattta
     2281 aaatggcttt tgaaatgcgc atcaatgcta atgcaatacc atagtatgtt gaccctttag
     2341 cttggataat ttcataagca gcgtcacgtg tattcacata aatttcttcc gctttagctt
     2401 cgctaccagt ttgttctttt aatgtgtcat atactgaaat acctgctaca tttgcttgtg
     2461 accaaactgc aagttcagta tcaccatgct cgccaataat actagcgtca acacttgaag
     2521 gtgcaacacc aagttcttgg ctaattaaat attgtaaacg tgcactgtcc aatacagtac
     2581 ctgaaccgat aacacgctct gctggtaatc cagtatattc ttttacaaat cttgttaaaa
     2641 tgtctacagg gtttgccgcg attaagaaat atccatcaaa gccactatcc ataacactct
     2701 taacgatgct cttcataatc ttagtatttt tttcaactaa ttgtaaacgt gtttcacctg
     2761 gcttttgagg tgcaccagct gtaataacaa ctaaatctgc atctttacag tcttcgtatt
     2821 cacctgcttt cacatcaact ggtgaaggac tgtggactgt accatggttt aaatcttgaa
     2881 catctgcttt tactttgtct tttgcaatgt caataattac aaattcatca gcaacacctt
     2941 gcgtaaccat tgcaaaggca tagcttgatc ctacagatcc atctccgatt aatacaacct
     3001 ttttaccaaa tgttttcata attatgtctc ctaacctaat tgaatttatt ttacacgtga
     3061 ctacacgtaa aaatatctat actgaaatta aagattaaca attttatttt attataatat
     3121 tatgctacca aattacaaac ataaaactga gatgtaagcg gagtcaaatt acactctatc
     3181 gccaataaat attgatatct caagctttag cacgtaattt gaaatttgtg acaattttta
     3241 gcattctaaa attgcgtaac gacactattt aaaggcttta acaaactatt gagctttcta
     3301 tttttttcat tttaaaaagg atggctattc gacttaaatg acttactata taacctccta
     3361 ttttccgcct taccctcatc atatttgatg catcatttta tagtcaaaga attacatttc
     3421 atacaaacta gccaatcaga cataaacatg aaattgattt taaaaatata atgtgcaaaa
     3481 ttgttagaaa attagcaaaa aatagtactt attcatttaa tatctgttat aatcagtttt
     3541 atcatttaaa aaatctatgg atgttatcaa gacaagtcat taatacgtaa cataaagttg
     3601 tgatttttca gaatttttag acaaaatatt gattatttgt gctatgatat aacatattaa
     3661 aaaacaccgt atagaattgt ttatgaatgt ttgggaggaa agtttatggg aagttttttc
     3721 aataaaatag cacgaaaaga ggatccggct atctatcaaa ataaagatgg tcatttaaag
     3781 cgtacacttc gggtgcgtga tttcttagct ttaggtgtag gaacaattgt atcgacatct
     3841 atctttacgc tacctggcat tgttgctgca gaacatgcag gaccggccgt tgcgttatca
     3901 ttcttactcg ctgctattgt tgctggttta gttgcattta cttatgcaga aatggctgcc
     3961 gctatgccat ttgcaggttc agcctattct tgggtcaatg tattatttgg tgaatttttt
     4021 ggatgggttg ccggttgggc tctattagct gaatatttta tcgccgtagc ctttgttgca
     4081 tcaggattct cagcgaattt acgcggactt gtgaaaccaa ttggcatcga attacctgca
     4141 gcattatcaa atccatttgg tacaaatggc ggttttatcg atattattgc tgctatcgtt
     4201 attttattaa ctgcattatt actatcacgt ggtatgtcgg aagcagctcg tatggaaaat
     4261 attttagtta ttttaaaagt attagctatt attttatttg tcatcgtagg tttaacagca
     4321 ataaatgtta gtaactatgt gccatttatt ccagaacaca aagtaactgc tacaggtgac
     4381 tttggtggat ggcaaggcat atatgctggt gtttcaatga ttttcttagc gtatatcggt
     4441 ttcgattcta tcgcagcaaa ctcagcagaa gcacttgatc ctcaaaagac aatgcctaga
     4501 ggtattcttg gttctttaag cgttgctatc gtattattta ttgctgtagc acttgtgtta
     4561 gttggtatgt tccattactc acaatacgca aacaatgctg aacctgttgg ttgggcttta
     4621 cgtcaaagtg gtcatggtgt tgtagcagct attgttcaag ctatctctgt tatcggtatg
     4681 tttacagcat taattggtat gatgttagca ggctcacgtt tactttattc atttggacgt
     4741 gacggcttat taccttcatg gttaagccac ttaaacgaca aacatttacc taatcgcgca
     4801 ctagttatac ttactattat tggtgtttta attggttcta tgttcccatt cgcattttta
     4861 gcacaattaa tttcagcagg tacacttgtt gcatttatgt tcgtttcatt agcaatgtat
     4921 cgtttgagaa agcgtgaagg taaagatcta ccaattcctg catttaaatt acctttatat
     4981 cctgtgttac cagcaattac atttgtttta gtattgctag tattctgggg attaggattc
     5041 gaagcgaaat tatacacttt aatttggttt attgttggta tcatcctata tttaagttat
     5101 ggacttagac attctaagaa aaatgacgta gctgaatacc acccaccaaa ataaaaaata
     5161 aaaaaaatca tttcccatct gattaacatc cagatgtgga aatgattttt tattttatat
     5221 taagataatc gctattacca accttgtcca gatatgtcat attgatctaa gttacctgct
     5281 tccaaagcag tcagtgcatc ttctatagtg tttaacgccg tgtctaattg ctcatatgtt
     5341 attaccaatg gcggttggaa tcgcaacaca tttcctgcta cagctataat aactacgcca
     5401 tgctcaaagc agtaattaca aattttaagt gccgcactgg catcacgtgt tttgagtttt
     5461 ttgtcggaaa caatatcaat accaatgctc agacctttac ctctaacatc gcctacacta
     5521 ttgtatttag atacccattg atccattcgt ttcctaacat attccccttt ttccgcacta
     5581 gcctgaagaa gcgactgatc ttcaatcatt tgaattgtgg ctaatgcagc ttcacaacta
     5641 actggattag cacctgttgt aaataaatgt gctggtgctt ctaaacaatt cataatctct
     5701 ttgcgtccaa caattgctga cataggcata ccacctgcta aggattttcc gaaagtgatt
     5761 aaatcaggcg taaaattaaa atgcgagact gaactccatg tacctgttct cccaaaacct
     5821 tgttgaatat catcgacagc gattaaaata ccatgttcac gacaaatctt ttctaacgct
     5881 tcaaaatacc ctggaactgg ttctaaaagt ccaccatcgc cttgtatcgt ttcaataaca
     5941 atacatgcta cttcgtcagc aggtacatac ttcgcaaaca tttcttttaa gggtgctaaa
     6001 tattcttcta ctgaattagc ttgtggctgc tcgtacatac cacgatattt atctggaaac
     6061 ggaatatgat aaaaaccatt caataacgga ccataatgtt tgcgcatatt taaactaata
     6121 gctgacatag acaatgagcc aaaagttgaa ccatgatatg cattagtgaa actaatgata
     6181 taaggacgcc ctgtatatgc tctggcaaat ttaatgatgc catcatttgc gtctgatccg
     6241 gttaatccga aggtcactct tttttcaaaa tctccaggtg caatctcaca aagcttctta
     6301 gctaaacgta ccaacggttc atgatacata tatgctggtg tataatgaat aaatttatca
     6361 acttgtgcct ttatcgcttc tgttacttct ctaggtgcat gacctacgtt ttgagaactc
     6421 gcactcgata acaaatcgat atatgtcttc ccctcaatat caaccaatgt tgctccatat
     6481 ccatgatcaa tcactaacgg ataatattta atacgtcctg atttcgcaaa ataatgttca
     6541 tcctcttgaa ttaattgatg tgctttactc atatacattc ccctcccatt gacagtatta
     6601 atgtgcaagc aaagtggcct ctcttaaatc gattataaaa tccgatttaa aatcctgctc
     6661 aaataccgtc atgcctctat gaatactatc caatatacta ataaaattca gatttgtaaa
     6721 tatattctga taattaagaa ttttatatca atctactttg tttatgcgca taattttgtt
     6781 cgattaggga attgtttact atattccatt tgactctgag gtgatacgaa tgcgtttaaa
     6841 agtattgttc catttcattg ctgctatttt catatcattc atgttgctat ggatgaccat
     6901 gttgtttgac ttaataagca atcaatcaca tttaaaagct ttattattga atttagattt
     6961 tctaattcct tctgacaata caccatatat tctagaaata atatgtcact tactaattgg
     7021 tagcgtcatt tattttgtat ttgtgttgct gtttcatact tctaaaaggc tttactatct
     7081 atgttatatc cctttatttt tcttatttat agcattgtat ccctttttag tatttattgc
     7141 gcaacgtcca atttttcaat ttagtgtgac agaattaatc ggttggatta ttacacatat
     7201 tttctttatg tcattaatgg cgttagtcat acctagaata aaataatatt tttaattaaa
     7261 ctgacaatta tttttcacat tttatacact tttcaaggtt tatgacttga gaaatgttct
     7321 catttttatc atactggagg tgtattgaaa agttaaggag tggaaagtat gaataaagag
     7381 caattagaaa aaatgaaaaa tggaaaaggc tttattgccg cattagacca aagtggtggt
     7441 agtacaccaa aagcacttaa agaatatggt gtaaacgaag atcaatatag caatgaagac
     7501 gaaatgttcc aacttgttca cgatatgcgt acacgtgtgg taacttcacc ttcattctca
     7561 ccagataaaa ttttgggtgc tattcttttc gaacaaacaa tggatcgcga agtagaaagc
     7621 aaatacactg cagattactt agctgataaa ggtgttgttc cgttcttaaa agtagacaaa
     7681 ggtcttgctg aagagcaaaa tggtgttcaa ttaatgaaac caatcgacaa cttagacaat
     7741 ttattagacc gtgcaaacga acgtcacatt tttggtacaa aaatgcgttc taacatttta
     7801 gaattaaatg agcaaggtat caaagacgtt gttgaacaac aatttgaagt tgctaaacaa
     7861 attattgcta aaggtttagt tccaattatc gaaccagaag ttaatattaa tgcaaaagac
     7921 aaagctgaaa ttgaaaaagt attaaaagct gaacttaaaa aaggtttaga tagcttaaat
     7981 gctgatcaat tagtaatgtt gaaattaaca attcctactg aaccaaactt atacaaagag
     8041 ttagctgaac atcctaatgt tgttcgtgtc gttgtattat caggtggtta cagcagagaa
     8101 aaagcaaatg aattacttaa agataacgct gaacttatcg caagtttctc acgtgcatta
     8161 gctagtgatt taagagctga tcaatctaaa gaagaattcg ataaagcttt aggtgatgct
     8221 gtagaatcaa tctacgacgc atctgtaaac aaaaactaat ttatacatta aactattttt
     8281 tgatataaac tactaaaaag gactcataca tgtgctttaa aggcattgtg tatgagtctt
     8341 ttttgattaa agttgatttt gccgtatgta ttagattacg taaaaaaatc ggacagctga
     8401 aaaatcttta agatttctca gttgtccgat tttaaatata gctattagaa tttagttttc
     8461 gtagtaacct aattctaagt cttttgaagt ttgtttacgg atttttctca ttaatttttc
     8521 gtcttcaatt aatgattcac cgtatgatgg aatcattttc ttaattttag gtgcccattc
     8581 agttttgtat tcagggaagt tacgttctaa tacttctaac gcaactgaaa ctgaagttga
     8641 agcccctggt gattcaccta ataatgcaat tacagtgtgg tcttgtgagt taaccacttc
     8701 tgtaccgaat tggatgaatc ctttaccgtg ttcaggtgta tctttgataa cttgtacacg
     8761 tttaccagca gtgtataatt gccaatcttc attacgtgct tctggataga aagtacgtaa
     8821 gtggttcata caaccttctt ttgtcataat tacttggtca aatgagtatt taattaaagg
     8881 taagttttta actgctgctg ctaataaagt tgtaatgttg tatgttttaa cagacttgaa
     8941 taaatctaag ttagaaccat ttttcaagaa tttaggtcca acattagcaa atggtccaaa
     9001 taataatgtt ctttgaccat caatgtaacg cgtatctaaa tgaggtacag tcattggtgg
     9061 tgtaccaggt ggctctttac cataaacttt ggcatcgtgt tgttcaataa cttgtgggtt
     9121 tgtacaagct aagaattgac cactgatagg gaatccaccc aaatgtttac tttcagggat
     9181 acctgttttt tgtaataatg gaattgctcc accgccagca ccgatgaata cgtagtcagt
     9241 tacttgtttg aatttctcac cagttaggcg atttttaaca gtaacttccc attgaccatt
     9301 tgataattgt tcaaaatcaa caacttcatg gttaaattgc actgtagcat ttggatgtgc
     9361 ttcaatgctt ttagccattt tacgtgttaa ttcaccgaag tttacatctg taccttcgtc
     9421 aattttactt gccgccatga taccagggtt atcttcacgg cctttcatca tcaatggaat
     9481 ccattttttc attacttcga tgtcttcagt atattcgata ttatcgaaca tagggaaagc
     9541 tttcatcgct tcgtaacgat cttttaagaa tttaacattg tttttacctc taacataact
     9601 gatgtgtggt aatggattga taaattctct tgggttctcg atgctaccgc ttttcactaa
     9661 gtgaccccag aattgttttg aaatctcaaa ctcttcgtta atcactttcg ctttttcgat
     9721 gtcgatagaa ccatcaggtt gtaaaactgt gtagttcaac tcacataatg ctgcatgacc
     9781 cgtaccagca ttatttcttt cgtttgaact ttcgattgca ggacgatcca agcgttcgta
     9841 aacgtggata ttccagtctg gctcaatttc ttttaacatt gaaccaaatg ttgtgctaag
     9901 tacaccggct ccaattaaaa cgatgtcttt actattagac ttagccattg gtttcacctc
     9961 tccaaaaatt gtaaatgtgt atcatcaata tgaaagttac ataaaactga catatttctt
    10021 taaaatatca acgccattga taacttcctg ttttaattga tacgctgtaa caaaatacta
    10081 tagttagtgc ttacatgtat atgttaaagc aagcagtggt aaatgtaaat tataattatt
    10141 cattaacttt gcaatatatt aaatctt
//
LOCUS       DALAGD010000099         9612 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.100, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000099 DALAGD010000000
VERSION     DALAGD010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9612
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.100"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(374..526)
                     /locus_tag="O2701_002244"
                     /pseudo
     CDS             complement(374..526)
                     /locus_tag="O2701_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500733.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
     gene            complement(526..1017)
                     /locus_tag="O2701_002245"
     CDS             complement(526..1017)
                     /locus_tag="O2701_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCZ4294119.1"
                     /translation="MVSDFESNDKITEVELLMHYNPKVINSKIKAMRSQIESLYHLNM
                     NHVITNDNDMLVSVSYPLDKLVLYIIEEKDKLEYYMKTAQDRLNLFKNIIKNYSENEQ
                     QDVMRYMLSSGKVKNEGVIERLKVDIYKVESRKRQERQNQREELHRIEFNKHLEQVKK
                     ELS"
     gene            complement(1201..2259)
                     /locus_tag="O2701_002246"
     CDS             complement(1201..2259)
                     /locus_tag="O2701_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HCZ4294120.1"
                     /translation="MKEFKEQFGYQLSNFDDMDIKGYANLYQKDIGKDVSMIEQGLKQ
                     LSITETEVLLPEQINKKLLGVLNMNEVNQSSNTWVGTLTKQLVSSENNFNIQELPTAR
                     KEVFKELLVNCELPQSLREVITITDDEHVESIPSLTYIKDKLATNGIELSLNGSSKYF
                     DRREGHIYTEVSDSVVHGSDRTLDDLLKEIFINECVAYETTLLLDKNNSSGLIDKGNQ
                     DLSLYNQGIKEVSNTSIYDGIKQAMKDIPQTFRRNVSIVMNTEHHDKLIKELAQMGLG
                     GLAGDLSNLFNVTNVVVTDDAHDVFVGDFGHAIYAKYEPIMYNKKKQALKGIYQFALN
                     YVFDIKIIPELLRIVNIK"
     gene            complement(2246..2452)
                     /locus_tag="O2701_002247"
     CDS             complement(2246..2452)
                     /locus_tag="O2701_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294121.1"
                     /translation="MLDHTNESVKRVFKYRDKQELLNKYHELLDKYFLHNISFYEEDK
                     NNERIYGLVVHTKNKLGDEDYERV"
     gene            complement(2677..3021)
                     /locus_tag="O2701_002248"
     CDS             complement(2677..3021)
                     /locus_tag="O2701_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCZ4294122.1"
                     /translation="MNRNRMKQIILEYIKNNDSTSFVEIENVFEEQGFKYKGNGAYTS
                     GNHKNIIFWMGWNEEAFNIVADLKRDGLIEMQICPPMYYLIDGKGLRLPIVKNKNIKI
                     DHWLPVTFSLVN"
     gene            complement(3692..6064)
                     /locus_tag="O2701_002249"
     CDS             complement(3692..6064)
                     /locus_tag="O2701_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCZ4294123.1"
                     /translation="MAIKKKDRIIGVKELEIPQELKLVPNWVLWRAEWNEKQQNFGKV
                     PYSINGYRASTTNKKTWCDFESVSIEYEVDEQYSGIGFVLSDGNNFVCLDIDNAIDKK
                     GQINSELALKMMQLTYCEKSPSGTGLHCFFKGKLPDNRKKKRTDLDIELYDSARFMTV
                     TGCTIGQSDICDNQEVLNTLVDEYFKENLPANEVVREESNTNIQLSDEDIINIMMKSK
                     QKDKIKDLLQGTYESYFESSSEAVQSLLHYLAFYTGKNKQQMERIFLNYNNLTDKWES
                     KRGNTTWGQLELDKAIKNQKTIYTKSIDEFNVIPQGSKDVKQLLNQLGHEERTKMEEN
                     WIEEGKRGRKPTTISPIKCAYILNEHLTFILFDDEENTKLAMYQFDEGIYTQNTTIIK
                     RVISYLEPKHNSNKADEVIYHLTNMVDIKEKTNSPYLIPVKNGVFNRKTKQLESFTPD
                     YIFTSKIDTSYVRQDIVPEINGWNIDRWIEEIACNDNQVVKLLWQVINDSMNGNYTRK
                     KAIFFVGDGNNGKGTFQELLSNVIGYSNIASLKVNEFDERFKLSVLEGKTAVIGDDVP
                     VGVYVDDSSNFKSVVTGDPVLVEFKNKPLYRATFKCTVIQSTNGMPKFKDKTGGTLRR
                     LLIVPFNANFNGIKENFKIKEDYIKNQQVLEYVLYKAINLDFETFDIPDASKKMLEVF
                     KEDNDPVYGFKVNMFDQWTIRKVPKYIVYAFYKEYCDENGYNALSSNKFYKQFEHYLE
                     NYWKTDAQRRYDNEELAKRIYNFNDNRNYIEPIESGKNYKSYEKVKLKAI"
     gene            complement(6156..6404)
                     /locus_tag="O2701_002250"
     CDS             complement(6156..6404)
                     /locus_tag="O2701_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1474 family protein"
                     /protein_id="HCZ4294124.1"
                     /translation="MNWEIKNLMCDLKILKEKFEDLKDNHGSHFEDLYLHEPNHTLNK
                     DDAIKEGFSYHERRIHNDQMFDALEKASSFADQSQDNA"
     gene            complement(6405..6788)
                     /locus_tag="O2701_002251"
     CDS             complement(6405..6788)
                     /locus_tag="O2701_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCZ4294125.1"
                     /translation="MEIKQKYQLSKVVKILEVVLYEEDKFQSDKDYHYQDKALYEYAL
                     KLVHNGLFNILAELDFEDEAFLILDEVTMTLSDVMKETQHVYRYSVIDEKGEHKHTTD
                     RKGHVIGMLEWALDYIVGNIEVEEL"
     gene            complement(6790..6993)
                     /locus_tag="O2701_002252"
     CDS             complement(6790..6993)
                     /locus_tag="O2701_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCZ4294126.1"
                     /translation="MRKQVIITKTVVGWYNIKDTQHNLMLNIPPKVFEQYFPDVSKDV
                     QVACLEMDLSKITEIKNKKKVGS"
     gene            complement(6960..7133)
                     /locus_tag="O2701_002253"
     CDS             complement(6960..7133)
                     /locus_tag="O2701_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCZ4294127.1"
                     /translation="MKMYLTYICLVSLLTILLLAISNMYVAFSVYGMMVTYGFNLTGE
                     ITTCENKLLLQKQ"
     gene            complement(7145..7417)
                     /locus_tag="O2701_002254"
     CDS             complement(7145..7417)
                     /locus_tag="O2701_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCZ4294128.1"
                     /translation="MSRTKLHDVPAKENTITEPKQVVVNPLFAKPNALASILGISYSS
                     VNRILKEWEKDSKGVDDLYYSLSSTMIVISIPRFEEYMKARHKKWM"
     gene            complement(7410..7673)
                     /locus_tag="O2701_002255"
     CDS             complement(7410..7673)
                     /locus_tag="O2701_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ4294129.1"
                     /translation="MVLNLKRLRAERIACGITQDEMAHKMGWKTRTPYAKRENGIVDI
                     GANEFIKMAKILGYETNNLDIFFTNNVPGKERKNILKGGELNV"
     gene            7866..8198
                     /locus_tag="O2701_002256"
     CDS             7866..8198
                     /locus_tag="O2701_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ4294130.1"
                     /translation="MRTNDEIITIIKTSMKEQNMSLSELARRVGVAKSAVSRYLNLTR
                     EFPLNRAEDFAKVLGIKTEYLLGFAEREESTKQDTIAAHLDGDFTEEELIEIRKYAEL
                     VRKAHRNQ"
     gene            8210..8668
                     /locus_tag="O2701_002257"
     CDS             8210..8668
                     /locus_tag="O2701_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCZ4294131.1"
                     /translation="MYLYEKMVIENKEIPIDDGKSLGNFEGLYDNGVILINKNLSETR
                     KAEVLYEELAHHKLTYGNILDQSKWINRKFENYARRHGFISAVPLREIVEAYNYGVRN
                     LYELSEYLQLSEKYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI"
     gene            8685..9413
                     /gene="seq"
                     /locus_tag="O2701_002258"
     CDS             8685..9413
                     /gene="seq"
                     /locus_tag="O2701_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type Q"
                     /protein_id="HCZ4294132.1"
                     /translation="MNKIFRVLTVSLFFFTFLIKNNLAYADVGVINLRNFYTNYQPET
                     LQGVSSGNFSTSHQLEYIDGKYTLYSQFHNEYEAKRLKDHKVDIFGISYSGLCNTKYM
                     YGGITLANQNLDKPRNIPINLWVNGKQNTISTDEVSTQKKEVTAQEIDIKLRKYLQNE
                     YNIYGFNKTKKGQEYGYQSKFNSGFNKGKITFHLNNETSFTYDLFYTGTGQAESFLKI
                     YNDNKTIDAENFHLDVEISYEKTE"
     gene            9437..>9612
                     /locus_tag="O2701_002259"
     CDS             9437..>9612
                     /locus_tag="O2701_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HCZ4294133.1"
                     /translation="MKKLIVIILINIITLSVSNSASAQGDIGIDNLRNFYTKKDFVDL
                     KDVKDNDTPIANQL"
BASE COUNT         3041 a         1694 c         1243 g         3634 t
ORIGIN      
        1 tttcattttc taaatctctt ttagcactac tcggtacttc cattcctctt cttcttagaa
       61 ttttcaattg tttattatga ctttcaaatg gcttcataaa tttaccccta taaaagtagc
      121 cataacccga atagagttat ggctagatcg ttatatatat aatacattta attttatcgg
      181 ttgtaaataa ataagaaata acaaaaagag atattttaca caattacctc ctttatttta
      241 ttgctactcc tcaaacccac caatattatc aataaacact ggtgttgtta catttaagtc
      301 tactttctca gtaaataagc tatggtatca aactaattag tattaattta taaatagcat
      361 agcttcattt tcttcaatcc tctaacggta tatcatccac aatcacagta tgattatggt
      421 tagcgttaga tacatcttgc actgccttat ctaattcctc atcatctccg tcccattcac
      481 caatattaat gaatatagga atattcccgt taatatcatg cttatctatg aaagttcttt
      541 tttaacttgt tctaaatgct tattaaactc tattcgatgt aattcttctc gctggttttg
      601 tcgttcttga cgctttctac tttcaacttt ataaatatct acttttaaac gttcaatgac
      661 tccttcattt ttaactttcc cactacttaa catgtacctc ataacatcct gttgttcatt
      721 ttcagaatag tttttgatga tatttttaaa caaatttagc ctatcctgag ctgttttcat
      781 gtaatattca agtttatctt tctcttctat aatataaagt actaatttat ctaatggata
      841 tgatacagat accagcatat cgttatcatt cgtgatcaca tggttcatgt tcaagtgata
      901 taagctttct atttgtgatc tcatcgcttt aattttgcta ttaatgactt tcggattgta
      961 atgcattaaa agttctactt cagtgatttt atcattgctt tcaaagtcag ataccaaata
     1021 accacacctt tacatatcga atttaatatt tataagtatc tgaaaaaggg ataaatcaag
     1081 ttatccccct ttcataaaag tttatccgcg ttgcaagcga agggcccccg ccggtacccg
     1141 gcgaaaaaac attttaagcc gatgggcagg gggctataaa attttattta aataattttt
     1201 ttatttaata ttaactatgc gcaataactc tggaattatt tttatatcaa acacatagtt
     1261 caatgcaaat tgataaatac cttttaatgc ttgtttcttt ttgttataca taataggttc
     1321 gtatttagca tatatggcat gtccaaagtc tccaacaaag acatcatgtg catcatcagt
     1381 aaccactaca ttagtcacat taaataggtt acttaaatca cctgcaagtc cacctaatcc
     1441 catctgtgcc aactctttga tgagcttatc atgatgttct gtgttcatca caatagatac
     1501 attacgccta aacgtttgtg gtatatcttt catcgcttgc ttgataccat cgtatattga
     1561 tgtgttactt acttctttga taccttgatt ataaagtgat aagtcttggt tacctttatc
     1621 aattaaacca cttgaattat tcttatctaa taacaatgtt gtttcatatg cgacacactc
     1681 attaataaat atctctttaa gtagatcatc taatgttcta tcagagccat gaactacact
     1741 gtctgatact tctgtgtaga tatgtccctc acgtctatca aagtatttac tactgccatt
     1801 aagtgatagc tcaataccgt tagtagctaa cttatcttta atgtatgtta gtgatggtat
     1861 agattcaaca tgttcatcat cagtaatggt aatcacctca cgtagtgatt gaggtagctc
     1921 acaattcacc aataactctt taaatacttc tttacgtgca gttggtaact cttgaatatt
     1981 aaagttattc tcactactaa ctaattgttt tgttaaggta cctacccatg tattacttga
     2041 ttgatttact tcattcatat ttaacactcc taataatttt ttgttaattt gctctggtaa
     2101 taaaacttct gtttcagtaa tacttaattg ctttaacccc tgttcaatca tacttacatc
     2161 tttacctata tccttttgat atagattggc atatccttta atatccatat catcaaagtt
     2221 agatagttga tacccaaatt gttctttaaa ctctttcata atcttcatct cctaatttgt
     2281 ttttagtatg aacaactaaa ccatatatac gttcgttgtt tttatcttct tcatagaacg
     2341 aaatgttatg aagaaagtat ttgtctaata attcgtgata cttatttaat agttcttgct
     2401 tatctctata cttaaacaca cgttttacag attcatttgt atgatctagc acgtcttgcc
     2461 tctctccttt taatctgata gatatacatt ttaataagtt caaaatcagc acgctttttt
     2521 gtatagcgcc ttacaacgcg atttatatat ttcgtattca ttaattgaac gttcacaggt
     2581 gtatgtttga ttatttgata tgcgattctt ttatgattaa taaatataca cactcccata
     2641 tctatacttg atattttaaa tgcttatctg catgttttaa tttactaaac taaatgttac
     2701 tggtagccaa tgatctattt ttatattttt attctttaca attggtagcc ttagaccttt
     2761 accatcaata agatagtaca ttggcggaca aatttgcatt tcaattagcc cgtcgcgttt
     2821 taaatctgcc acaatgttaa acgcttcttc attccaaccc atccaaaata taatattctt
     2881 atggttgcca ctggtatatg cgccgttacc tttgtattta aaaccttgtt cttcaaatac
     2941 attctcaatc tctacaaatg aagtgctgtc attgttcttt atatattcta agatgatttg
     3001 tttcattcta tttctattca tgttactcta gtcctccaat ctatccagtt actaaagcaa
     3061 ccacgttaga tactaaaaac cactgaattt atgtttttca ataaccactg taaacacagt
     3121 gatgtcaata catacaggct gttggttact gagttactga aaattcacgg tattttatat
     3181 aatcttttat taccttaatt ctctattata catttctact aaaaagaaat aatttatagt
     3241 aactcagtaa ctttttgtca aaaagactaa tatgaaagta aaaactgtgg ttactgaatt
     3301 ctagaatttt agtatctttt agtgacttaa acgtttttta gtaactttag tatcttaggc
     3361 aattttgtat aactgcaagt ctcttccgat tatgtgcaac tgaaatttac agctatgcaa
     3421 ctcgttttaa attttccagt tgcaattttt ttcttactgt cacaaggggt taatccactt
     3481 ttgcaactat gcaaccgaaa tcacacaaat tagtttatat aatttttgat actatttctt
     3541 atttatattt tggttattta cttttctact aataagttat agattttgag ttgctcagtt
     3601 gcataagtgc cctaaaccct tgatataact gtgattatag gtgcaacccg ttttaaaatt
     3661 ttcggttgca ttcggttgca ttcggttgca actatattgc ttttagcttc accttttcat
     3721 acgatttata gttttttcca ctttcaatag gttcaatgta atttctattg tcattaaagt
     3781 tgtatatcct cttagcaagt tcttcattgt catatcttcg ctgtgcatca gttttccaat
     3841 aattctctaa ataatgttca aattgtttat aaaacttgtt tgaactcaat gcattatagc
     3901 cattttcatc acaatattct ttataaaatg cgtatacaat atatttcggc acttttctaa
     3961 tagtccattg atcaaacata tttactttaa acccataaac tggatcgtta tcttctttaa
     4021 atacttcaag cattttttta gatgcatcag gaatgtcaaa ggtttcaaaa tctaaattaa
     4081 ttgctttata aagcacatac tctagcactt gctgattttt tatatagtct tctttgattt
     4141 taaaattctc tttaatgcca ttaaaattgg cattaaacgg tactatcaat aaccttctta
     4201 aagtcccgcc tgttttgtct ttaaatttag gcattccatt cgttgattga ataactgtac
     4261 acttaaaagt cgctctatat aagggtttat ttttaaactc aaccaacact ggatcgccag
     4321 taactacact tttaaagttt gaagaatcat cgacatacac accaactggt acatcgtctc
     4381 caattactgc tgtcttgccc tctaacacac tcaatttaaa acgttcatca aactcattca
     4441 cttttaagct agcaatattg ctataaccta ttacattaga caataattct tgaaacgtcc
     4501 ctttaccatt atttccatca ccaacaaaga aaatagcctt tttacgagtg tagttaccat
     4561 tcattgagtc attaattact tgccacaata atttaacaac ctgattatca ttacaagcaa
     4621 tctcttctat ccaccgatct atattccagc catttatttc aggtactata tcttgcctta
     4681 catacgatgt atctatttta ctggtaaata tataatcagg tgtaaatgat tctagttgct
     4741 tcgttttacg gttaaataca ccatttttaa ctggtattaa gtatggtgag ttagttttct
     4801 cttttatatc taccatattg gttaaatgat aaataacttc atcagctttg ttgctattat
     4861 gtttgggctc taaataggaa atcactcgtt ttataattgt agtgttctgt gtatatatcc
     4921 cttcatcaaa ttgatacata gctaacttag tattttcttc atcatcaaaa agtataaatg
     4981 ttaaatgctc attcaaaata tatgcacatt ttatagggct aattgttgta ggctttcgcc
     5041 ctcgttttcc ttcttcaatc caattttctt ccatttttgt tctttcttca tgccccaatt
     5101 gattcaacaa ctgtttaaca tctttactcc cctgtggtat aacattaaat tcatctatcg
     5161 atttagtata aattgtcttt tgattcttta tagctttatc taactcaagc tgtccccaag
     5221 tcgtattacc tcgtttactt tcccatttat ccgtaagatt attgtagttt aaaaatatac
     5281 gctccatttg ctgtttgttt ttacctgtgt aaaacgctaa gtagtgtaat aagctttgta
     5341 ctgcttcgct tgaactctca aaatatgatt catatgtgcc ttgtaataga tctttaattt
     5401 tatctttttg tttagatttc atcataatgt ttataatatc ttcatcagat aattgtatat
     5461 tggtattaga ttcctctctc acaacctcat ttgctggcaa attctcttta aaatattcat
     5521 caactagagt atttaatact tcttgattat cacaaatatc actttgacca attgtgcatc
     5581 ctgtaacagt cataaacctt gctgaatcat ataactctat gtctaaatct gttcttttct
     5641 ttttacggtt atctggtagt ttacctttaa agaaacaatg taatcctgta ccacttggag
     5701 acttttcaca atatgtgagt tgcatcattt ttagtgctaa ttcagaattg atttgtcctt
     5761 ttttatcaat tgcattatca atatctaggc aaacaaaatt attaccatca cttaatacaa
     5821 aacctatacc gctatattgc tcatcaactt catattcaat acttacactt tcaaagtcac
     5881 accatgtttt tttattggtt gtactagctc tgtaaccatt aatactatat ggtacttttc
     5941 caaaattttg ttgcttctcg ttccattcag ctcgccataa tacccaattg ggcactaatt
     6001 ttaattcttg cggtatttct aattctttaa ctccaataat tctatctttt ttcttaattg
     6061 ccacagaatg cctcctttct aatctgattt ctctgtattt tcaatgcaac taatgttaaa
     6121 atattaatgg tatgatttta taaattttta tttttttatg cgttatcttg gctttggtcg
     6181 gcgaaagatg acgctttttc taatgcatca aacatttgat cattgtgaat acgtctctca
     6241 tgatatgaaa atccttcttt aatagcatcg tctttattta aggtatgatt tggctcatgt
     6301 agatataaat cttcaaaatg cgagccatga ttatccttta aatcctcaaa cttttctttc
     6361 aatatcttta aatcacacat taaattttta atttcccaat tcatttataa ttcctccact
     6421 tcaatatttc ccacaatgta atctaatgcc cactctaaca ttccaatcac gtgtcctttg
     6481 cgatctgttg tatgtttatg ttcacctttt tcgtctatga cactataacg gtaaacgtgt
     6541 tgtgtttctt tcatgacatc acttagcgtc attgttactt catcaagaat taaaaatgct
     6601 tcatcttcaa aatctaattc agcaagaata ttgaacaatc cattatgaac taactttaaa
     6661 gcatattcat ataatgcttt atcctgataa tgatagtcct tatcggattg aaacttatct
     6721 tcctcgtata atactacttc taatatttta accacttttg ataattgata tttttgtttg
     6781 atttccatct taactaccta cttttttctt atttttaatt tctgtaattt ttgataaatc
     6841 catttctaaa cacgcaactt gaacatcttt actaacatca ggaaagtact gttcaaatac
     6901 ttttggcggt atatttaaca ttaaattatg ttgagtatct ttaatgttgt accagccaac
     6961 tactgttttt gtaataataa cttgttttcg cacgttgtaa tctctcctgt taaattaaat
     7021 ccataagtta ccatcatgcc gtacacacta aaagcgacat acatgttaga tattgctagt
     7081 aataatattg ttaacaatga aactaagcag atataagtta agtacatttt cattgccttg
     7141 cctcctacat ccatttttta tgacgcgcct tcatgtactc ctcgaatcgc ggaatactga
     7201 taacaatcat tgttgatgat aacgagtaat ataaatcatc aacaccttta gaatcttttt
     7261 cccactcttt taaaatgcga ttcaccgaac tatatgaaat tcccaaaata ctagctagtg
     7321 cattaggttt cgcaaacaaa ggattcacta caacttgctt tggttctgta attgtatttt
     7381 ctttagctgg tacatcatgc aattttgttc tagacattta attcacctcc ttttaagatg
     7441 ttttttcgtt cttttccggg aacgttattg gtaaaaaaaa tgtctaggtt atttgtttca
     7501 taacctaata tttttgccat tttaataaat tcattcgctc caatatctac tattccattt
     7561 tctctctttg cataaggcgt tcttgttttc caccccattt tgtgagccat ttcatcttgc
     7621 gtaataccac aagctattct ttccgctctc aatcttttta aatttagtac cacgtttaca
     7681 cctcctatcg ttctcgttcg agaactaatt acaatttacc atttacgttc tcgttcgtca
     7741 acaattttta cttaaaaaaa ttcaaaaagt tttttcttcc ttatatattg tattcttttg
     7801 ggaacggtgt tataatcaaa tcgttcacaa ataagaacaa atattcaatt caggagatac
     7861 aagaaatgag aactaatgat gaaataatca caataattaa aacatcaatg aaagaacaaa
     7921 atatgtcact aagtgaatta gctcgtcgtg taggtgtagc aaaatcagca gtatcaagat
     7981 atttaaattt aactagagag ttcccattga atcgtgctga agattttgcg aaagtacttg
     8041 gaataaaaac agaatattta ttaggatttg ctgaacgcga agaatctaca aaacaagata
     8101 ctatcgccgc gcacttagat ggagatttta cagaggaaga attaattgaa attagaaagt
     8161 atgcggagtt agttagaaaa gcacatcgaa atcagtaagg ggttattgca tgtatttata
     8221 cgaaaagatg gttattgaaa acaaagagat accaattgat gatgggaaat ctttaggtaa
     8281 ttttgagggt ctctatgaca atggggttat tttaattaat aaaaatttgt cagaaacacg
     8341 taaagcagaa gtgttatatg aggaacttgc ccaccacaag ttgacgtatg gcaacatttt
     8401 agaccaatca aaatggatta atagaaaatt tgaaaattat gcacgtagac acggctttat
     8461 ctcagctgtt ccattacgtg aaattgtaga agcttataat tatggcgtac gtaacttgta
     8521 tgagttgtcc gagtacctac aactgagtga aaaatacata ttagaagcga tagaacaata
     8581 taaaaagata tacggtattg gtacccacta cggcgagtat tcaattacat ttgagccgtt
     8641 gagagttttt aaatataaag aaatataaca aaggagaaat gaaaatgaat aaaatattta
     8701 gagtactcac tgttagcttg tttttcttca catttttaat aaaaaacaat ctagcatatg
     8761 ctgatgtagg ggtaatcaac cttagaaact tttatactaa ttatcaacca gaaacgcttc
     8821 aaggagttag ttctggaaat ttttctactt ctcatcaatt agagtatatt gatggaaaat
     8881 acactttata ttcacagttt cataatgaat atgaagcgaa gagattaaaa gatcataaag
     8941 tagatatctt tggaataagt tactcaggtc tttgtaatac aaaatatatg tatggcggaa
     9001 ttacgttggc gaatcaaaat ttagataaac ctagaaatat acctattaat ctctgggtca
     9061 atggtaagca aaacactata tctacagacg aggtttctac tcaaaaaaaa gaggtaactg
     9121 ctcaagagat tgatattaag ttacgaaaat acctacaaaa cgaatacaat atatatggtt
     9181 ttaataaaac aaaaaaaggt caggaatatg gatatcagtc aaaatttaat tctggattta
     9241 acaaaggaaa aattactttc catttaaata atgaaacttc ttttacatac gatttgtttt
     9301 acaccggaac tggtcaagca gagagttttt taaaaattta caatgataat aaaactatag
     9361 atgcagagaa ttttcatttg gatgtagaga tttcatatga gaagactgaa taaatagaat
     9421 ttaaggagaa aatgcaatga aaaaattaat agttatcata ttaataaata taataacttt
     9481 aagtgtctct aatagtgcca gcgctcaagg tgatatagga attgataatc tcaggaattt
     9541 ttatacaaaa aaagactttg tagatttaaa agatgtaaaa gacaatgata ctcctatagc
     9601 taatcaacta ca
//
LOCUS       DALAGD010000100         9457 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.101, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000100 DALAGD010000000
VERSION     DALAGD010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9457
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.101"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..205
                     /locus_tag="O2701_002260"
     CDS             <1..205
                     /locus_tag="O2701_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294134.1"
                     /translation="SEKMTKKEFIDIPVLFDDEGNFRIKQVIDYKKIIGKSYVNGKYI
                     ETKLGKVHYSKTGFHVVPYIKKE"
     gene            212..403
                     /locus_tag="O2701_002261"
     CDS             212..403
                     /locus_tag="O2701_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LSM domain protein"
                     /protein_id="HCZ4294135.1"
                     /translation="MNLQSYIGKLVKLTLTNNKILIGKVIDFDDKVDNFDGYNSIEID
                     TGRITYDISENKIKTIVLQ"
     gene            complement(952..1977)
                     /locus_tag="O2701_002262"
     CDS             complement(952..1977)
                     /locus_tag="O2701_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HCZ4294136.1"
                     /translation="MISFENDYLEGAHEKVLKRLVDTNLVQASGYGFDQFTAQAIEKI
                     KDTIDCPNATIRFLVGGTQTNQVVINSMLESYEGVISADTGHVAVHEGGAIEYSGHKV
                     ITIPSKEGKVSASDVETYMETFKSDFKKDHMVFPGMVYISHPTEYGTLYSKSELEELC
                     KVCKQYQLPLFMDGARLGYGLMSDQSDMTIKDIAKYCDVFYIGGTKIGALCGEAIVFT
                     KNNEPKQFTTRIKHHGALLAKGRLTGIQFLELFTDNLYFNISRHAIEMANKMKDGFKN
                     KGYRLYFDSPTNQQFFILSNEKIAELEQKVKFAVWEKYDDQHRVVRFATSWATTEENL
                     NKLLELI"
     gene            2247..2444
                     /locus_tag="O2701_002263"
     CDS             2247..2444
                     /locus_tag="O2701_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294137.1"
                     /translation="MEQIKLKTFTAETLELLEKNINAFLSSEEATNLKLVNITIKEIE
                     ERTFPNNEEEFNAILTLSVNK"
     gene            2502..3983
                     /gene="cls"
                     /locus_tag="O2701_002264"
     CDS             2502..3983
                     /gene="cls"
                     /locus_tag="O2701_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCZ4294138.1"
                     /translation="MRFTFSNDLGTLFTIILAIGFIINLVLAFIIIFLERNRRTASST
                     WAWLFVLFVLPLIGFILYLFFGRTVSARKLNKNNGNVLTDFDGLLKQQIESFDKGNYG
                     TDNKQVQKHHDLVRMLLMDQDGFLTENNKVDHFIDGNDLYDQVLKDIKNAKEYIHLEY
                     YTFALDGLGKRILHALEEKLKQGLEVKILYDDVGSKNVKMANFDHFKSLGGEVEAFFA
                     SKLPLLNFRMNNRNHRKIIVIDGQLGYVGGFNIGDEYLGLGKLGYWRDTHLRIQGDAV
                     DALQLRFILDWNSQAHRPQFEYDVKYFPKKNGPLGNSPIQIAASGPASDWHQIEYGYT
                     KMIMSAKKSVYLQSPYFIPDNSYINAIKIAAKSGVDVHLMIPCKPDHPLVYWATFSNA
                     SDLLSSGVKIYTYENGFIHSKMCLIDDEIVSVGTANMDFRSFELNFEVNAFVYDENLA
                     KDLRVAYEHDITKSKQLTKESYANRPLSVKFKESLAKLVSPIL"
     gene            4158..5066
                     /locus_tag="O2701_002265"
     CDS             4158..5066
                     /locus_tag="O2701_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4294139.1"
                     /translation="MIKLIQISNINKSFNKRCVLKNISFDIEQGKCIALIGKNGAGKS
                     TLIDILIGNVNANSGEIFDKDKLLQSENRSIMFQKTMFPDQLKVIEIINLYQSFYENP
                     LPLEEIIELTKFDSSQLNQFVNKLSGGQQRLLDFVLSLIGQPQLILLDEPTSTMDIEI
                     REYFWSIIENLKEDNRTILYTSHYIEEVERMSDKIILIENGEIILNDSTSHIRTNQQS
                     QITLSDEYIRKLKLDKDDLVIQKNHNGTIKIITSNVNDTILYLQQLHINLDDIEIQKV
                     SIVDSYFNNKKQRGSNYDTKLLENRI"
     gene            5035..5766
                     /locus_tag="O2701_002266"
     CDS             5035..5766
                     /locus_tag="O2701_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4294140.1"
                     /translation="MILSYLKIEFKVIMRKKTTLILSILFPVIFYILFTSILELPEDV
                     KPKFYKEYMYSMTVYSLLSFSLLTFPLDIINEKQNEWRQRLMVTPFTFTSYYISKVVK
                     TMLQFAIAILVIFMVGHFYKGVAMSAVQWLESGIFLWLGASLLITFGILFSLLNDIQK
                     TSALANIVTIGLAVLGGLWFPINTFPNWLQHVAHVLPSYHLRKLGVDIASNHHINLIS
                     FAIILLYALGSIIAVYCISHFKRAE"
     gene            5770..6861
                     /locus_tag="O2701_002267"
     CDS             5770..6861
                     /locus_tag="O2701_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCZ4294141.1"
                     /translation="MKFLKDTSIAEISSIVYLIFPIAGIFFNEVYGPKWLYIISVIVF
                     SLSYLILVIVNNRLNTLMFYILLIIHYFIICYFVFSVHPMLSLFFFYSAFAVPFTFKN
                     NVKKTATNLFILTMIICTIITYLLYNNYFVAMMVYYVVISLIMLDNFKKMKNREYQKE
                     IAEKNRHINTLIAEQERHRIGQDLHDTLGHVFASLSLKSELAYKLIDADVEKVKAELL
                     AINKLSRESLNKVREIIDDVKLPSFIEEIDSIRKVLKDADIDFTFENKELAQVLSPTK
                     QSMLVMITREAINNVIKHANASKVHGKLKTVNNHKLLLMIEDDGKGIDSDCEVKSISQ
                     RVQHLNGTLAVDSTNGTKIIIEISTGGIA"
     gene            6858..7460
                     /locus_tag="O2701_002268"
     CDS             6858..7460
                     /locus_tag="O2701_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCZ4294142.1"
                     /translation="MTSLIIAEDQNMLRQAMVQLIKLHGDFEILADTDNGLDAMKLIE
                     EYNPNVVFLDIEMPGMTGLEVLAEIRKKHLNIKVIIVTTFKRPGYFEKAVVNDVDAYV
                     LKERSIEELVETINKVNNGEKEYSATLMTSFFVDKNPLTPKEQIVLREIGNGLSSKEI
                     SEKLFLTDGTVRNYTSVIIDKLFADNRFDAWKKANEKGWI"
     gene            complement(7573..7761)
                     /locus_tag="O2701_002269"
     CDS             complement(7573..7761)
                     /locus_tag="O2701_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HCZ4294143.1"
                     /translation="MNISNENTVTLIAVIIAIIIGIFLQIFFKLPLIVTAVLSILLGI
                     FVGFIVYLIVSFYNKRKN"
     gene            7900..8433
                     /locus_tag="O2701_002270"
     CDS             7900..8433
                     /locus_tag="O2701_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCZ4294144.1"
                     /translation="MKSNKSLAMIVVAIIIVGVLAFQFMNHTGPFKKGTNHETVQDLN
                     GKDKVHVQRVVDGDTFIANQNGKEIKVRLIGVDTPETVKPNTPVQPFGKEASNYSKKT
                     LTNQDVYLEYDKEKQDRYGRTLAYVWISKDRMYNKELVEKGLAREKYFSPNGKYRNVF
                     IEAQNKAKQQKLNIWSK"
     gene            complement(8576..9430)
                     /locus_tag="O2701_002271"
     CDS             complement(8576..9430)
                     /locus_tag="O2701_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294145.1"
                     /translation="MSFYVVLIIIIVALIGILVLNQRYSNSKIDTEVYARKQLIKKNK
                     ALSAENAELRSQMLSSNNDVGHHAYKNAKRELRKILDSYLENGKLKYYDIIVTSNLAT
                     KHPFFEYARSFDFIIVSDIGLINVDVKSWGEKTFYHFDVPDEHDTEISNSNIEKVVGH
                     YISQQYHDQFNSSRKSIYTFTETVQPNRVIYDFYDYDPYQLAANNAKALKDHIEQNFN
                     FKVQSTGVIYFSDGTVNIIQGSEERDKYVDTVSTKSSLRRIISEAIELSKHPLNKEQV
                     DQITAIFK"
BASE COUNT         3479 a         1249 c         1459 g         3270 t
ORIGIN      
        1 aagtgaaaag atgactaaaa aagaatttat tgatattcca gttttgtttg atgatgaagg
       61 gaattttaga ataaaacaag ttatagatta taaaaagata ataggtaaat cttatgtgaa
      121 tggtaaatat attgaaacga aattaggtaa ggttcattat tctaaaactg gttttcatgt
      181 agttccgtat attaaaaagg agtgattaaa tatgaattta cagtcataca taggcaaatt
      241 agtcaaatta acgcttacta ataataaaat attaattgga aaagtgatag actttgatga
      301 taaagttgat aattttgatg gttataattc gatagaaatt gatacaggta gaattacata
      361 tgatatatca gaaaataaaa ttaaaactat tgttttacaa taatagcatc ctttctacac
      421 agataaagag aaagcggtgc tattttttgc gcttttataa caactaagga tacaagacac
      481 ataacttcaa caatatcctc atctaaacct atcgatttcc taagctcaat tttaaacaca
      541 aagtttattt gaaatgatgc acacctataa aaatcttaat catcgaaaag tcacaaacat
      601 ctccatatta aaaacttaaa tagtgcattc catttaaact aaacatcgat attcctacca
      661 atatagactt agtaattcga ttgttaatac attactttca taaaataact acaaccatca
      721 taatacttct caaatgtcgt caaattaaag taaatacagc acaaattaac atttttaaat
      781 atataaaata aaacaagata ctcaataatc tgtagtacct tgttttggaa taatgtaatt
      841 ttatatcatc aacggctaca aatgagcagt ttcatatata aataaacaca tctactcaac
      901 tgatcttaat taattaataa atgatttacc agatgacata taacagcatt attagattaa
      961 ttctaatagt ttatttaaat tttcttcggt tgtcgcccaa ctggttgcga atctaacaac
     1021 acgatgttga tcatcgtatt tttcccaaac agcaaattta actttttgtt ctaactctgc
     1081 tattttctcg ttacttaaaa taaaaaattg ttgattggtt ggagaatcaa agtaaagacg
     1141 atagccttta tttttaaacc cgtctttcat cttatttgcc atttcgatag catgtctgct
     1201 tatattaaaa tataaattgt ccgtaaataa ttctaaaaat tgtatgcctg ttaaccgtcc
     1261 ttttgctaaa agggcaccgt gatgcttgat tcgagtggta aattgtttcg gttcattatt
     1321 tttcgtaaaa acaatggctt ccccgcataa tgcacctatc ttcgtaccac ctatataaaa
     1381 tacatcacaa tatttagcga tgtctttaat agtcatatct gattggtcac tcatcaatcc
     1441 ataccctaat cgtgcaccat ccataaataa tggaagctga tattgcttac atactttgca
     1501 taactcttcc aattctgatt tagagtataa tgtgccatat tctgtaggat gagaaatata
     1561 taccattcct gggaatacca tatggtcctt tttaaaatca cttttaaatg tctccatgta
     1621 agtttcaaca tctgaagcac taacttttcc ttccttagag ggtatagtaa ttactttatg
     1681 tccactatat tcaattgcac cgccctcatg cacagcaaca tgaccagtgt ctgctgaaat
     1741 gaccccttcg taactttcta acattgaatt aataacaacc tgattggttt gtgttccacc
     1801 tactaaaaaa cgaattgtag catttggaca gtcaattgta tctttaatct tttcaattgc
     1861 ctgagctgtg aattgatcaa agccatatcc cgaagcttgt acaagatttg tatctactaa
     1921 tcgttttaat actttttcat gagcaccttc taaataatca ttttcgaatg aaatcactac
     1981 atttccccct aaaactaata tcaacatttt aataagataa accaatttca aaactagttc
     2041 gatatttaaa atgtattatg aatggttaaa gtttgtatcg cattatcgcg aagttgaata
     2101 aatatattat acatcaaaca atacattatg aaaattaatt aattttttat cacagcgact
     2161 tttcatgctg gaacacatgt attaatgatt gaattttact gttaaaagtt ctatgattta
     2221 ggaaatagag aaaagagggg atttaaatgg aacaaattaa acttaaaact tttacagctg
     2281 agactttaga gttattagaa aaaaacatta atgctttttt aagttctgaa gaagctacaa
     2341 atttaaaatt agtaaatatt actattaaag aaatagaaga aagaacattc ccaaataatg
     2401 aagaagaatt caatgcaatt ttaactttat ctgtgaataa ataatatgac gtgaaaggtg
     2461 caaaatgttt ttttgtgcct ttctttatag gagaaaagac tatgcgattt acattttcaa
     2521 acgatttagg aacgttattt actattattt tagccattgg attcatcatt aatttagtat
     2581 tggcttttat tattatcttt ttagaaagaa ataggcgtac agcgagttca acttgggcat
     2641 ggctatttgt actttttgtc ttaccattga ttggttttat tctttacttg ttttttggta
     2701 gaaccgtttc ggcacgcaaa ttgaataaaa acaatggtaa cgtgttaacg gatttcgatg
     2761 gacttttaaa acaacaaata gaaagctttg ataaaggtaa ttatggtact gataacaaac
     2821 aagttcaaaa acatcatgat ttagtacgta tgcttttgat ggatcaagat ggttttttaa
     2881 ctgaaaataa taaagttgat catttcattg atggaaatga tttatatgat caagttttaa
     2941 aagatattaa aaatgcaaaa gaatatatcc atttagagta ctatactttc gctttagatg
     3001 gtttaggtaa aagaatttta catgctttag aagaaaaatt gaaacaaggt ctagaagtaa
     3061 aaatattata tgatgatgtt ggatctaaaa atgttaagat ggcaaatttt gatcatttta
     3121 aatcgttagg tggagaagtt gaagcatttt ttgcttcaaa attaccgtta ttgaatttca
     3181 gaatgaataa tagaaatcat agaaaaatca tcgtaatcga tggtcaacta ggttatgtcg
     3241 gaggatttaa cattggtgat gaatatctag gattaggaaa attaggatat tggagagata
     3301 cgcatttacg tatacaaggg gatgcggttg atgcactgca gttgcgattt attttagact
     3361 ggaattcgca agcgcaccgt ccacaatttg aatatgatgt taagtatttc cctaaaaaga
     3421 acggaccatt gggcaattca ccaattcaaa tagctgcaag tggcccggct agtgactggc
     3481 atcaaattga atacggttat acaaaaatga ttatgagtgc aaagaaatct gtatatttac
     3541 aatcaccata tttcattccg gataattcat atataaatgc cattaaaatt gctgctaaat
     3601 caggtgtaga tgtacattta atgattccat gtaagccaga tcatccatta gtatattggg
     3661 cgacattttc aaatgcctct gacttattat caagtggtgt taaaatttat acgtatgaaa
     3721 atggatttat acattctaaa atgtgcttaa ttgatgatga aatcgtatca gtgggcacag
     3781 caaatatgga ctttagaagt tttgaattaa attttgaagt aaatgccttt gtatatgatg
     3841 aaaatcttgc taaagattta agggtggctt atgaacatga tattacaaaa tcaaaacaac
     3901 taaccaaaga atcatatgcc aatagaccgc tgtctgttaa attcaaagaa tcgttagcaa
     3961 aattagtttc gccaatttta taatttattt gtaaggagtc tcgattatag aggctctttt
     4021 tattttgccc aaaattaaaa taacgataaa tatattatgt aaatctaata atgacatttg
     4081 tcatttcaaa tctatgacaa agtgttctat tttaaatcta ataaatttac taatattaaa
     4141 ttaagaaaaa aatagaggtg attaaattga ttcaaatatc taatatcaac aagtcattta
     4201 ataaaagatg tgttctaaaa aatatttcgt tcgatattga acaaggtaaa tgtatcgctt
     4261 taattggaaa aaatggtgct ggaaagtcaa cgttaattga tatattaatt ggtaatgtta
     4321 atgctaattc tggtgagata tttgataaag acaagttatt acaaagtgaa aatcgcagta
     4381 taatgttcca aaaaacgatg tttccagatc aattaaaagt tattgagatt atcaacttat
     4441 atcaatcatt ttacgaaaat ccattaccat tggaagaaat aatagaactg acgaaatttg
     4501 attctagtca actgaaccaa tttgtaaata aactttctgg tggtcaacaa cgattactcg
     4561 attttgtatt atctttaatc ggacaaccac aattgatctt attagatgaa ccaacatcga
     4621 ctatggatat agaaattaga gaatattttt ggtcaattat tgaaaattta aaagaagata
     4681 atcgaacgat actctataca tcgcactata ttgaagaagt cgaacgtatg tcagacaaaa
     4741 ttattctcat tgaaaatgga gaaataatac ttaatgattc aacgtcacat attagaacca
     4801 atcagcaatc tcagattacg ttatccgatg aatatataag aaagttaaaa ctagataaag
     4861 atgatttagt tattcaaaaa aatcataatg gcactatcaa aattattact tcaaatgtaa
     4921 atgatacgat tttatatctt caacaacttc atattaattt ggatgatatt gaaatacaaa
     4981 aagtctcaat tgttgattca tacttcaaca ataaaaagca aaggggatct aattatgata
     5041 ctaagttact tgaaaatcga atttaaagtt ataatgcgta aaaaaacaac attaatatta
     5101 tctattttat ttcctgttat attctatata ttatttactt cgatattgga attgccggaa
     5161 gatgttaaac ctaaatttta taaagagtat atgtatagta tgacggttta tagtttgtta
     5221 agttttagtt tactaacttt tccattagat attattaatg aaaaacaaaa tgaatggcgc
     5281 caaagattaa tggtaacacc atttactttt actagttatt atatttcaaa agtagtgaaa
     5341 actatgctgc aatttgcaat agcgatatta gttattttta tggttggaca tttttataaa
     5401 ggtgttgcaa tgagtgcagt tcaatggtta gagtcaggaa tatttttatg gttaggtgcg
     5461 tctctattaa taacttttgg catattattt tctttgttaa atgatattca aaaaacaagt
     5521 gctttagcta atatcgtaac aattggttta gcagtattag gtggattgtg gtttccgata
     5581 aacacatttc caaattggct tcaacatgtt gctcatgttt taccgagcta tcatttgcgt
     5641 aaactaggtg tagatattgc ttcaaatcat catatcaatt taatatcatt tgctataata
     5701 ctcttgtatg ctttagggag tataatagca gtatattgta ttagtcattt taaaagggcg
     5761 gaataaaata tgaaattttt aaaagatact tcaattgctg aaatatcgtc tatagtttat
     5821 ctgatttttc ctattgccgg tatatttttt aatgaagtat atggtcccaa atggttgtat
     5881 attatatcag tcattgtctt ttcgttgtcg tatcttatat tagttatagt aaataataga
     5941 cttaatacat taatgtttta cattttgttg attattcatt attttattat ttgttatttt
     6001 gttttcagtg tacatccaat gctaagtttg tttttctttt atagtgcttt tgccgttcca
     6061 tttactttta aaaataatgt taaaaaaacg gcaactaatc ttttcatact aacaatgatt
     6121 atatgtacaa taataacgta cttattgtat aacaactatt ttgttgcaat gatggtttat
     6181 tatgtcgtta tatcgttaat aatgctagat aattttaaaa aaatgaaaaa ccgtgaatat
     6241 caaaaagaaa tagcagaaaa aaatagacat attaatacat taattgctga acaagagcga
     6301 catagaattg gtcaagactt acatgatacg ttagggcatg tgtttgcaag tttatcatta
     6361 aaatcagaat tagcttataa actaatagat gctgatgtag aaaaagtaaa agctgaatta
     6421 ttagcaatta ataaattatc tcgtgaatca ttgaacaaag ttcgagaaat tattgatgat
     6481 gtaaaattac catcatttat tgaagagatt gatagtatac gtaaagtttt aaaagatgct
     6541 gatattgatt ttacatttga aaataaagaa ttagcgcaag tattaagtcc tactaaacaa
     6601 tctatgttag ttatgattac gcgtgaagcg ataaataatg ttattaaaca tgcaaatgct
     6661 tcaaaagttc atggtaaatt aaaaactgta aacaatcata aattactgct tatgattgaa
     6721 gatgatggca aaggtatcga tagtgattgt gaggtgaaaa gtatttcaca gcgtgtacaa
     6781 catttaaatg gaactttagc agtcgactca acaaatggaa ctaaaataat cattgaaatc
     6841 tcaacaggag gaatagcatg acatctttaa ttattgcaga agatcaaaat atgttacgac
     6901 aggcaatggt tcaattaatt aaactacatg gtgattttga aattttagca gatactgata
     6961 atggtctcga tgcaatgaaa cttattgaag aatataatcc taacgttgtt tttttagata
     7021 tagaaatgcc aggcatgact ggacttgaag ttttagcgga aattagaaaa aagcatttga
     7081 atattaaagt gattattgta acaactttta aaagaccggg atactttgaa aaagcagttg
     7141 tgaatgatgt ggatgcatat gttttaaaag aacgttctat agaagaattg gtggaaacca
     7201 ttaataaagt aaataacgga gagaaagaat atagcgccac attgatgact tcattttttg
     7261 tagataaaaa cccattaacg cccaaagaac aaattgtatt aagggaaatt ggcaatggtt
     7321 taagtagtaa agaaataagt gaaaaattat ttttgacaga tggaacagtt agaaattata
     7381 catctgttat aattgataaa ttatttgcag ataatcgttt tgatgcttgg aaaaaggcaa
     7441 atgaaaaagg ctggatctaa atacaaacaa aaaagtattg agtgttttga tttaagagac
     7501 ttggaaagtg agtcaataat caaaatctta ctcaatactt ttattttggt actcgcaatt
     7561 tagatttgtt aactaatttt ttcgcttatt ataaaacgaa acaatcaagt aaacgataaa
     7621 gcctacaaag atacccaata aaatagatag tactgccgtc actattaatg gtaatttgaa
     7681 aaatatttgt aggaaaatac caatgataat tgcgataatt actgcaatta atgtgactgt
     7741 attttcattt gaaatgttca tttattttca ctccttaaca ataacattat atcatgctat
     7801 agctttccaa aatattgaaa tatgtagata tggctattga cgatatttct taacttttat
     7861 atgattaatc ggaatgaaaa aagagaagta ggtggcaata tgaagtcaaa taaatcgctt
     7921 gctatgattg tggtagccat cattattgta ggtgtattag catttcaatt tatgaatcat
     7981 acgggtcctt tcaaaaaggg gacgaatcat gaaactgtac aagatttaaa tggtaaagat
     8041 aaagtacatg ttcaaagagt tgtggatggt gatacattta ttgcaaatca aaatggtaaa
     8101 gaaattaaag ttaggcttat aggggttgat acgccagaaa cggtgaaacc gaatacgcct
     8161 gtacaaccat ttggcaaaga agcatcaaat tatagtaaga agacattaac aaatcaagat
     8221 gtttatttag aatatgataa agaaaaacaa gatcgctatg gtagaacatt ggcgtatgta
     8281 tggataagta aagatcgtat gtacaataag gaattagtgg aaaagggact tgctagagag
     8341 aagtattttt caccaaatgg caaatataga aatgtattta tagaagcaca aaataaagct
     8401 aaacaacaga aattaaatat ttggagtaaa taattaaata ggtgattata tatattaaag
     8461 taaaatatac ttgaagcatc gatttattat taccataaat ttatatatag aaataattta
     8521 aaggcataat aaccatagtg cgtatcgagc gtaatggatt attatgcctt tgattttact
     8581 tgaatatagc tgtgatttga tcaacctgtt ctttatttaa agggtgttta gatagctcta
     8641 tggcttcgct aataatacgt cgtaacgaag attttgttga aactgtatca acatatttat
     8701 ctctttcttc agaaccttga atgatattaa ctgtaccatc actaaagtaa ataacaccgg
     8761 tactttggac tttaaagtta aagttttgtt caatatgatc ctttaatgct ttcgcattat
     8821 ttgctgctaa ttgatacgga tcataatcat aaaaatcata aataacacga tttggttgaa
     8881 ctgtttctgt aaaagtatag atagactttc ttgatgaatt aaactgatca tgatattgct
     8941 ggctaatgta atgaccgaca actttttcaa tattactatt gcttatttct gtatcatgct
     9001 catcaggtac atcaaagtga taaaatgttt tttcacccca gctttttaca tcaacgttta
     9061 ttaaaccgat atctgaaaca atgataaaat caaatgaacg tgcatactcg aaaaacggat
     9121 gtttagtagc taaattgctt gtaacgataa tatcgtaata ttttaattta ccattttcga
     9181 gatagctatc taaaatcttg cgtaattctc ttttagcatt tttgtatgca tggtggccaa
     9241 catcattatt agaactcagc atttgacttc ttaattcagc attttctgca cttaatgctt
     9301 tgttcttttt aataagttgc tttcttgcat aaacttcggt atctatttta ctattactat
     9361 acctttgatt taaaactaat ataccaatta atgctacaat gataatgata agtacaacat
     9421 aaaaagacat tttttcacca atcctttttg acttctt
//
LOCUS       DALAGD010000101         9417 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.102, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000101 DALAGD010000000
VERSION     DALAGD010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9417
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.102"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            110..1006
                     /gene="xerC"
                     /locus_tag="O2701_002272"
     CDS             110..1006
                     /gene="xerC"
                     /locus_tag="O2701_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCZ4294146.1"
                     /translation="MNHIQDAFLNTLKVERNFSEHTLKSYQDDLIQFNQFLEQEHLEL
                     NTFEYRDARNYLSYLYSNHLKRTSVSRKISTLRTFYEYWMTLDENIINPFVQLVHPKK
                     EKYLPQFFYEEEMEALFKTVEEDTSKNLRDRVILELLYATGIRVSELVNIKKQDIDFY
                     ANGVTVLGKGSKERFVPFGAYCRQSIENYLEHFKPIQSCNHDFLIVNMKGEAITERGV
                     RYVLNDIVKRTAGVSEIHPHKLRHTFATHLLNQGADLRTVQSLLGHVNLSTTGKYTHV
                     SNQQLRKVYLNAHPRAKKENET"
     gene            1003..1548
                     /gene="hslV"
                     /locus_tag="O2701_002273"
     CDS             1003..1548
                     /gene="hslV"
                     /locus_tag="O2701_002273"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCZ4294147.1"
                     /translation="MSNTTLHATTIYAVRHNGKAAMAGDGQVTLGQQVIMKQTARKVR
                     RLYEGKVLAGFAGSVADAFTLFEKFETKLQQFSGNLERAAVELAQEWRGDKQLRQLEA
                     MLIVMDKDAILVVSGTGEVIAPDDDLIAIGSGGNYALSAGRALKRHASHLSAEEMAYE
                     SLKVAADICVFTNDNIVVETL"
     gene            1614..3017
                     /gene="hslU"
                     /locus_tag="O2701_002274"
     CDS             1614..3017
                     /gene="hslU"
                     /locus_tag="O2701_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HCZ4294148.1"
                     /translation="MDTAGIRLTPKEIVSKLNEYIVGQNDAKRKVAIALRNRYRRSLL
                     DEESKQEISPKNILMIGPTGVGKTEIARRMAKVVGAPFIKVEATKFTEVGYVGRDVES
                     MVRDLVDVSVRLVKAQKKSLVQDEATAKANEKLVKLLVPSMKKKASQTNNPLESLFGG
                     AIPNFGQNNEDEEEPPTEEIKTKRSEIKRQLEEGKLEKEKVRIKVEQDPGALGMLGTN
                     QNQQMQEMMNQLMPKKKVEREVAVETARKILADSYADELIDQESANQEALELAEQMGI
                     IFIDEIDKVATNNHNSGQDVSRQGVQRDILPILEGSVIQTKYGTVNTEHMLFIGAGAF
                     HVSKPSDLIPELQGRFPIRVELDSLSVEDFVRILTEPKLSLIKQYEALLQTEEVTVNF
                     TDEAITRLAEIAYQVNQDTDNIGARRLHTILEKMLEDLSFEAPSMPNAVVDITPQYVD
                     DKLKSISTNKDLSAFIL"
     gene            3042..3815
                     /gene="codY"
                     /locus_tag="O2701_002275"
     CDS             3042..3815
                     /gene="codY"
                     /locus_tag="O2701_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HCZ4294149.1"
                     /translation="MSLLSKTRELNTLLQKHKGIAVDFKDVAQTISSVTVTNVFIVSR
                     RGKILGSSLNELLKSQRIIQMLEERHIPSEYTERLMEVKQTESNIDIDNVLTVFPPEN
                     RELFIDSRTTIFPILGGGERLGTLVLGRVHDDFNENDLVLGEYAATVIGMEILREKHS
                     EVEKEARDKAAITMAINSLSYSEKEAIEHIFEELGGTEGLLIASKVADRVGITRSVIV
                     NALRKLESAGVIESRSLGMKGTFIKVKKEKFLDELEKSK"
     gene            3866..3958
                     /locus_tag="O2701_002276"
     CDS             3866..3958
                     /locus_tag="O2701_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294150.1"
                     /translation="MAVVNEMTLMIMLKSFLYFCLIFNQIDISH"
     gene            4157..4924
                     /gene="rpsB"
                     /locus_tag="O2701_002277"
     CDS             4157..4924
                     /gene="rpsB"
                     /locus_tag="O2701_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCZ4294151.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKKVDEAYNFLKQVSEDGGQVLFVGTKKQAQESVKSEAERAGQFYINQRWLGGLLT
                     NYKTISKRIKRISEIEKMEEDGLFEVLPKKEVVELKKEYDRLIKFLGGIRDMKSMPQA
                     LFVVDPRKERNAIAEARKLNIPIVGIVDTNCDPDEIDYVIPANDDAIRAVKLLTAKMA
                     DAILEGQQGVSNEEVAAEQNIDLDEKEKSEETEATEE"
     gene            4958..5071
                     /locus_tag="O2701_002278"
     CDS             4958..5071
                     /locus_tag="O2701_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294152.1"
                     /translation="MGDKILMLITFFKKKIEANYKYSIRVLAILPIIMLKS"
     gene            5106..5987
                     /locus_tag="O2701_002279"
     CDS             5106..5987
                     /locus_tag="O2701_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCZ4294153.1"
                     /translation="MATISAKLVKELREKTGAGMMDCKKALTETDGDIDKAIDYLREK
                     GIAKAAKKADRIAAEGLVHVETKGNDAVIVEINSETDFVARNEGFQELVKEIANQVLD
                     TKAETVEALMETTLPNGKSVDERIKEAISTIGEKLSVRRFAIRTKTDNDAFGAYLHMG
                     GRIGVLTVVEGSTDEEAARDVAMHIAAINPKYVSSEQVSEEEINHEREVLKQQALNEG
                     KPENIVEKMVEGRLRKYLQEICAVDQDFVKNPDVTVEAFLKTKGGKLVDFVRYEVGEG
                     MEKREENFADEVKGQMK"
     gene            6124..6846
                     /locus_tag="O2701_002280"
     CDS             6124..6846
                     /locus_tag="O2701_002280"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCZ4294154.1"
                     /translation="MAQISKYKRVVLKLSGEALAGEKGFGINPVIIKSVAEQVAEVAK
                     MDCEIAVIVGGGNIWRGKTGSDLGMDRGTADYMGMLATVMNALALQDSLEQLDCDTRV
                     LTSIEMKQVAEPYIRRRAIRHLEKKRVVIFAAGIGNPYFSTDTTAALRAAEVEADVIL
                     MGKNNVDGVYSADPKVNKDAVKYEHLTHIQMLQEGLQVMDSTASSFCMDNNIPLTVFS
                     IMEEGNIKRAVMGEKIGTLITK"
     gene            6865..7419
                     /gene="frr"
                     /locus_tag="O2701_002281"
     CDS             6865..7419
                     /gene="frr"
                     /locus_tag="O2701_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCZ4294155.1"
                     /translation="MSDIINETKSRMQKSIESLSRELANISAGRANSNLLNGVTVDYY
                     GAPTPVQQLASINVPEARLLVISPYDKTSVADIEKAIIAANLGVNPTSDGEVIRIAVP
                     ALTEERRKERVKDVKKIGEEAKVSVRNIRRDMNDQLKKDEKNGDITEDELRSGTEDVQ
                     KATDNSIKEIDQMIADKEKDIMSV"
     gene            7792..8562
                     /locus_tag="O2701_002282"
     CDS             7792..8562
                     /locus_tag="O2701_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HCZ4294156.1"
                     /translation="MFKKLINKKNTINNYNEELDSSNIPEHIAIIMDGNGRWAKKRKM
                     PRIKGHYEGMQTIKKITRVASDIGVKYLTLYAFSTENWSRPESEVNYIMNLPVNFLKT
                     FLPELIEKNVKVETIGFTDKLPKSTIEAINNAKEKTANNTGLKLIFAINYGGRAELVH
                     SIKNMFDELHQQGLNSDIIDETYINNHLMTKDYPDPELLIRTSGEQRISNFLIWQVSY
                     SEFIFNQKLWPDFDEDELIKCIKIYQSRQRRFGGLSEE"
     gene            8569..9351
                     /locus_tag="O2701_002283"
     CDS             8569..9351
                     /locus_tag="O2701_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCZ4294157.1"
                     /translation="MKVRTLTAIIALIVFLPILLKGGLVLMIFANILALIALKELLNM
                     NMIKFVSVPGLISAVGLIIIMLPQHAGPWVQVIQLKSLIAMSFIVISYTVLSKNRFSF
                     MDAAFCLMSVAYVGIGFMFFYETRSEGLHYILYAFLIVWLTDTGAYLFGKMMGKHKLW
                     PVISPNKTIEGFIGGLFCSLIVPLAMLYFVDFNMNVWILLGVTLILSLFGQLGDLVES
                     GFKRHFGVKDSGRILPGHGGILDRFDSFMFVLPLLNILLIQS"
BASE COUNT         3497 a         1296 c         1851 g         2773 t
ORIGIN      
        1 ccgattagtt acaattattt aattctcaaa agatttagaa ttgattatca aattactgta
       61 agccctttgc tgtatatgct acaattctta ttgatggagg gtaaatgtat tgaatcatat
      121 tcaagatgcg tttttaaata cattgaaagt tgaacggaat ttttcggaac acacattgaa
      181 atcatatcaa gatgacttaa ttcagtttaa tcaattttta gaacaagaac atttagagtt
      241 gaatactttt gaatacagag atgctagaaa ttatttgagc tatttatatt caaatcattt
      301 gaaaagaaca tctgtttctc gtaaaatctc aacgttaaga actttctatg aatattggat
      361 gacgcttgat gagaacatta ttaatccatt tgttcaatta gtacatccga aaaaagaaaa
      421 atatcttccg caattctttt acgaagaaga aatggaagcg ttattcaaaa ctgtagaaga
      481 ggacacttca aaaaatttac gggatcgagt tattcttgaa ttgttgtatg ctacaggcat
      541 ccgtgtttcg gaattagtaa atattaaaaa acaagatata gatttttacg cgaatggtgt
      601 taccgtatta ggaaaaggga gcaaagagcg ctttgtaccg tttggtgctt attgtagaca
      661 aagcatcgaa aattatttag aacatttcaa accaattcag tcatgcaatc atgattttct
      721 tattgtaaat atgaagggtg aagcaatcac tgaacgcggt gtacgatatg ttttaaatga
      781 tattgttaaa cgaacagcag gcgtaagtga gattcatccc cacaagctca gacatacatt
      841 tgcaacgcat ttattgaatc aaggtgcaga cctaagaaca gtacaatcgt tattaggtca
      901 tgttaatttg tcaacaactg gtaaatatac acacgtatct aaccaacaat taagaaaagt
      961 gtatctaaat gcacatcctc gagcgaaaaa ggagaatgaa acatgagtaa tacaacatta
     1021 catgcaacaa caatttatgc tgtaagacat aatgggaaag cagctatggc tggagatggg
     1081 caagtaacgc ttggtcaaca agtcatcatg aaacaaacgg caagaaaagt gcgacgttta
     1141 tatgaaggta aagtgttagc tggtttcgca ggtagtgtag cagatgcgtt tacgttattt
     1201 gaaaaattcg aaacaaaatt acaacagttt agtggtaact tagaaagagc tgctgttgaa
     1261 ttggcacaag aatggcgagg cgataaacaa ttacgtcaat tagaagctat gctaattgta
     1321 atggataaag atgctatttt agttgtcagt ggaactggcg aagttattgc tccagatgat
     1381 gaccttatcg ctattggatc aggaggcaac tacgcattaa gcgcaggacg tgcattgaaa
     1441 cgccatgcat cgcatttgtc tgctgaagaa atggcatatg agagcttgaa agtagcggct
     1501 gatatttgtg tctttaccaa cgataatatt gttgtcgaaa cactataata atcagagcac
     1561 gataaataat tacgagcaat taattttagt taaaagacgg aggaatgaaa ttaatggata
     1621 cagctggaat aagattaact ccaaaagaaa tcgtatctaa attaaatgaa tacatcgttg
     1681 gacaaaatga tgctaaacgt aaagtggcaa ttgccctacg taatcgatac agaagaagtt
     1741 tattagatga ggaatcaaag caagaaattt cacctaaaaa tattttgatg attggaccaa
     1801 ccggcgttgg taaaactgaa attgcaagaa gaatggccaa agttgtcggc gcgccattta
     1861 taaaagtaga agctactaaa tttactgagg taggttatgt aggacgagat gttgaaagta
     1921 tggttagaga tcttgttgat gtttcagtaa gattagtcaa ggcgcagaaa aaatcattgg
     1981 tacaagatga agcaacagct aaggccaatg aaaaacttgt taagttatta gttccaagta
     2041 tgaaaaagaa agcgtctcaa acgaataatc ctttagagtc acttttcgga ggtgcaattc
     2101 caaatttcgg acaaaataac gaagatgaag aagaaccacc tactgaggaa attaaaacaa
     2161 aacgttctga aattaagaga cagctagaag aaggcaaact tgaaaaagaa aaggtaagaa
     2221 ttaaagtcga acaagatcct ggtgctttag gtatgctagg tacaaatcaa aatcagcaaa
     2281 tgcaagagat gatgaatcaa ttaatgccta aaaagaaagt tgagcgagaa gttgctgttg
     2341 agacggcaag gaaaatctta gctgatagtt atgcggatga actaattgat caagaaagcg
     2401 ctaaccaaga agcgcttgaa ttagcagaac aaatgggtat catctttata gatgaaatcg
     2461 acaaagttgc gacgaataat cataatagtg gtcaagatgt ctcaagacaa ggtgttcaaa
     2521 gagatatttt acctatactt gaaggtagcg ttattcaaac caaatatggt actgtgaata
     2581 ctgaacatat gctgtttata ggtgctggag ctttccatgt atctaagccg agtgacttga
     2641 taccagaatt gcaaggtcgt tttccgatta gagttgaact tgatagttta tcggtagaag
     2701 attttgtaag aattttgaca gaaccaaaat tgtcattaat taaacaatat gaagcattgc
     2761 ttcaaacaga agaagttact gtaaacttta ccgatgaagc aattactcgc ttagctgaga
     2821 ttgcttatca agtaaatcaa gatacagaca acattggtgc acgtcgactt catacaattt
     2881 tagaaaagat gctagaagat ttatcattcg aagcaccaag tatgccgaat gcagttgtag
     2941 atattacccc acaatatgtt gatgataaat taaaatcaat ttcaacaaat aaagatttaa
     3001 gtgcatttat tctataaaaa tatacaaaag gagaaaaatt catgagctta ttatctaaaa
     3061 cgagagagtt aaacacgtta cttcaaaaac acaaaggtat tgcggttgat tttaaagatg
     3121 tagcacaaac gattagtagc gtaactgtaa caaatgtatt tattgtatcg cgtcgaggta
     3181 aaattttagg atcgagtcta aatgaattat taaaaagtca aagaattatt caaatgttgg
     3241 aagaaagaca tattccaagt gaatatacag aacgattaat ggaagttaaa caaacagaat
     3301 caaatattga tatcgacaat gtattaacag tattcccacc tgaaaacaga gaattattca
     3361 tagatagtcg tacaactatc ttcccgattt taggtggagg ggaaagatta ggtacattag
     3421 tacttggtcg agtacatgat gattttaatg aaaatgattt ggtactaggt gaatatgctg
     3481 ctacagttat tggtatggaa atcttacgtg agaagcatag tgaagtagaa aaagaagcgc
     3541 gcgataaagc tgctattaca atggcaatta attcattatc ttattctgaa aaagaagcga
     3601 ttgaacatat ctttgaagaa cttggcggta cggaaggcct attaatcgca tcaaaagttg
     3661 cagatagagt tggtattact agatctgtaa ttgtaaatgc actacgtaaa ttagaaagtg
     3721 ctggtgtaat tgaatcacgt tctttaggaa tgaaaggtac tttcattaaa gttaaaaaag
     3781 aaaaattctt agatgaatta gaaaaaagta aataagtcga tgagtctggg acataattca
     3841 atgtcctagt cgtgacgatg ttatcttggc ggtagtcaat gaaatgactt tgatgattat
     3901 gttgaagtca tttctttatt tttgtctaat tttcaatcaa attgatattt cacattaact
     3961 tgatagaatt acctttaaat ttatggaaaa atgcttgaaa aacatttgct ttagacacgg
     4021 cattatgtta tatttatttt cggctataaa gccaatacac acatatcaaa cgtgattgtg
     4081 taaagggtgc aatatttata tattgtcttt acaatagttt gctatggagg taattaacca
     4141 ataggaggaa tttataatgg cagtaatttc aatgaaacaa ttactagaag cgggtgttca
     4201 cttcggtcac caaacacgtc gttggaaccc aaaaatgaaa aaatatatct tcactgagag
     4261 aaatggtatt tatatcatcg acttacaaaa aacagtgaaa aaagtagacg aggcatacaa
     4321 cttcttgaaa caagtttcag aagatggtgg acaagtctta ttcgtaggaa ctaaaaaaca
     4381 agcacaagaa tcagttaaat ctgaagcaga acgtgctggt caattctaca ttaaccaaag
     4441 atggttaggt ggattattaa ctaactataa aacgatctca aaacgaatca aacgtatttc
     4501 tgaaattgaa aaaatggaag aagatggttt attcgaagta ttacctaaaa aagaagtagt
     4561 agaacttaaa aaagaatacg accgtttaat caaattctta ggcggaattc gtgatatgaa
     4621 atcaatgcct caagcattat tcgtagttga cccacgtaaa gagcgtaatg caattgctga
     4681 agctcgtaaa ttaaatattc ctatcgtagg tatcgttgac actaactgtg atcctgacga
     4741 aattgactac gttatcccag caaacgacga tgctatccgt gcggttaaat tattaactgc
     4801 taaaatggca gatgcaatct tagaaggtca acaaggcgtt tctaatgaag aagtagctgc
     4861 agaacaaaac atcgatttag atgaaaaaga aaaatcagaa gaaacagaag caactgaaga
     4921 ataatcaact gttgaatctg acttagatat agtttaaatg ggtgataaga tattaatgct
     4981 tatcaccttt tttaaaaaga aaatcgaggc aaattacaaa tattcaatta gagtattggc
     5041 aatcttgcct ataataatgc taaaatcata atatataaaa tgataactta ttggaggaat
     5101 aatgaatggc aactatttca gcaaaacttg ttaaagaatt acgtgaaaaa actggcgcgg
     5161 gtatgatgga ttgtaaaaaa gcgctaactg aaactgatgg tgacatcgat aaagcgattg
     5221 actacctacg tgaaaaaggt attgctaaag cagctaaaaa agcagaccgt attgcggctg
     5281 aaggtttagt acatgtagaa actaaaggta acgacgcagt tatcgttgaa atcaactctg
     5341 aaacagactt tgttgctcgt aacgaaggtt tccaagagtt agttaaagaa atcgctaatc
     5401 aagtattaga tacaaaagct gaaactgttg aagctttaat ggaaacaact ttaccaaatg
     5461 gtaaatcagt tgatgaaaga attaaagaag caatttcaac aatcggtgaa aaattaagtg
     5521 ttcgtcgttt tgctatcaga actaaaactg ataacgatgc tttcggcgct tacttacaca
     5581 tgggtggacg cattggtgta ttaacagttg ttgaaggttc aactgacgaa gaagcagcaa
     5641 gagacgttgc tatgcatatc gctgcaatca accctaaata tgtttcttct gaacaagtta
     5701 gcgaagaaga aatcaaccac gaaagagaag ttttaaaaca acaagcatta aatgaaggta
     5761 aaccagaaaa catcgttgaa aaaatggtgg aaggacgttt acgtaaatac ttacaagaaa
     5821 tttgtgctgt agatcaagac ttcgttaaaa accctgatgt aacagttgaa gctttcttaa
     5881 aaacaaaagg tggaaaactt gttgacttcg tacgctatga agtaggcgaa ggtatggaaa
     5941 aacgcgaaga aaactttgcg gatgaagtta aaggacaaat gaaataatct gtcataaagt
     6001 aaaacaagga agaagacacc tttaatgttg ctttattaaa atgtaaatca ttctaataaa
     6061 acgacaactg tgtcttcttt acttgtatat gttacatata ttcacgatag agaggataag
     6121 aaaatggctc aaatttctaa atataaacgt gtagttttga aactaagtgg tgaagcgtta
     6181 gctggagaaa aaggatttgg cataaatcca gtaattatta aaagtgttgc tgagcaagtg
     6241 gctgaagttg ctaaaatgga ctgtgaaatc gcagtaatcg ttggtggcgg aaacatttgg
     6301 agaggtaaaa caggtagtga cttaggtatg gaccgtggaa ctgctgatta catgggtatg
     6361 cttgcaactg taatgaatgc cttagcatta caagatagtt tagaacaatt ggattgtgat
     6421 acacgagtat taacatctat tgaaatgaag caagtggctg aaccttatat tcgtcgtcgt
     6481 gcaattagac acttagaaaa gaaacgcgta gttatttttg ctgcaggtat tggaaaccca
     6541 tacttctcta cagatactac agcggcatta cgtgctgcag aagttgaagc agatgttatt
     6601 ttaatgggca aaaataatgt agatggtgta tattctgcag atcctaaagt aaacaaagat
     6661 gcggtaaaat atgaacattt aacgcatatt caaatgcttc aagaaggttt acaagtaatg
     6721 gattcaacag catcctcatt ctgtatggat aataacattc cgttaactgt tttctctatt
     6781 atggaagaag gaaatattaa acgtgctgtt atgggtgaaa agataggtac gttaattaca
     6841 aaataaattt agaggtgtaa aataatgagt gacattatta atgaaactaa atcaagaatg
     6901 caaaaatcaa tcgaaagctt atcacgtgaa ttagctaaca tcagtgcagg aagagctaat
     6961 tcaaatttat taaacggcgt aacagttgat tactatggtg caccaacacc tgtacaacaa
     7021 ttagcaagca tcaatgttcc agaagcacgt ttacttgtta tttctccata cgacaaaact
     7081 tctgtagctg acatcgaaaa agcgataata gcagctaact taggtgttaa cccaacaagt
     7141 gatggtgaag tgatacgtat tgctgtacct gccttaacag aagaacgtag aaaagagcgc
     7201 gttaaagatg ttaagaaaat tggtgaagaa gctaaagtat ctgttcgaaa tattcgtcgt
     7261 gatatgaatg atcagttgaa aaaagatgaa aaaaatggcg acattactga agatgagttg
     7321 agaagtggta ctgaagatgt tcagaaagca acagacaatt caataaaaga aattgatcaa
     7381 atgattgctg ataaagaaaa agatattatg tcagtataaa actaatatac aatgacatat
     7441 taaaatgcca gtattaaacg ataatgtaac atttaaaatg ggcatgttta attaaatcaa
     7501 agatgcatgt gataatttaa attcacaatg agcataaaaa tggtgtttaa acaagttaat
     7561 taaacatata ctttataaat aataggcatt aggtatattg ctataataaa gttatgtaat
     7621 ttttaacctc agtatgtatg tcacatttct ggtgtaaact gtaccgagtc agactttggt
     7681 acagtttttt tatttgctta ttcaatgcat taaatgagta tgataaaatg ataatgattg
     7741 tttagtaact tatactatat gacagagatg atcaggctcg gaggaaagac catgtttaaa
     7801 aagctaataa ataaaaagaa cactataaat aattataatg aagaattaga ctcgtctaat
     7861 atacctgaac atatcgctat tattatggat ggtaatgggc gatgggctaa gaagcgaaaa
     7921 atgcctagaa ttaaaggtca ttacgaaggt atgcaaacaa taaaaaaaat tactagggta
     7981 gctagtgata ttggtgttaa gtacttaact ttatacgcct tttccactga aaattggtca
     8041 agacctgaaa gtgaagtaaa ttatattatg aatttgcctg tcaatttctt aaagacattc
     8101 ttaccggaac taattgaaaa aaatgtcaaa gttgaaacaa ttggatttac tgataagttg
     8161 ccaaaatcaa cgatagaagc aattaataat gctaaagaaa agacagctaa taataccggc
     8221 ttaaaattaa tatttgcaat taattatggt ggcagagcag aacttgttca tagtattaaa
     8281 aatatgtttg acgagcttca tcaacaaggt ttaaatagtg atatcataga tgaaacatat
     8341 ataaacaatc atttaatgac aaaagactat cctgatccag agttgttaat tcgtacttca
     8401 ggagaacaaa gaataagtaa tttcttgatt tggcaagttt cgtatagtga atttatcttt
     8461 aatcaaaaat tatggcctga ctttgacgaa gatgaattaa ttaaatgtat aaaaatttat
     8521 cagtcacgtc aaagacgctt tggcggattg agtgaggagt agtatagtat gaaagttaga
     8581 acgctgacag ctattattgc cttaatcgta ttcttgccta tcttgttaaa aggcggcctt
     8641 gtgttaatga tatttgctaa tatattagca ttgattgcat taaaagaatt gttgaatatg
     8701 aatatgatta aatttgtttc agttcctggt ttaattagtg cagttggtct tatcatcatt
     8761 atgttgccac aacatgcagg gccatgggta caagtaattc aattaaaaag tttaattgca
     8821 atgagcttta ttgtaataag ttatactgtc ttatctaaaa acagatttag ttttatggat
     8881 gctgcatttt gcttaatgtc tgtggcttat gtaggcattg gttttatgtt cttttatgaa
     8941 acgagatcag aaggattaca ttacatatta tatgcctttt taattgtttg gcttacagat
     9001 acaggggctt acttgtttgg taaaatgatg ggtaaacata agctttggcc agtaataagt
     9061 ccgaataaaa caatcgaagg attcataggt ggcttgttct gtagtttgat agtaccactt
     9121 gcaatgttat attttgtaga tttcaatatg aatgtatgga tattacttgg agtgacattg
     9181 attttaagtt tatttggtca attaggtgat ttagtggaat caggatttaa gcgtcatttc
     9241 ggcgttaaag actcaggtcg aatactacct ggacacggtg gtattttaga ccgatttgac
     9301 agctttatgt ttgtgttacc attattaaat attttattaa tacaatctta atgctgagaa
     9361 caaatcaata aacgtaaaga ggagttgctg agataattta atgaatctca gaactcc
//
LOCUS       DALAGD010000102         9347 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.103, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000102 DALAGD010000000
VERSION     DALAGD010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9347
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.103"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            154..606
                     /gene="argR"
                     /locus_tag="O2701_002284"
     CDS             154..606
                     /gene="argR"
                     /locus_tag="O2701_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCZ4294158.1"
                     /translation="MPKKSVRHIKIREIISNEQIETQDELVKRLNDYDLNVTQATVSR
                     DIKELQLIKVPIPSGQYVYSLPNDRKFHPLEKLGRYLMDSFVNIDGTDNLLVLKTLPG
                     NAQSIGAILDQINWEEVLGTICGDDTCLIICRSKEASDEIKSRIFNLL"
     gene            622..2301
                     /gene="recN"
                     /locus_tag="O2701_002285"
     CDS             622..2301
                     /gene="recN"
                     /locus_tag="O2701_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCZ4294159.1"
                     /translation="MLQTLSIKQFAIIEELEIQFSDGLTVLSGETGSGKSIIIDAIGQ
                     LIGMRASSDFVRHGEKKAVIEGIFDIDESKDAIHILKNMDIDVDEDFLLVKREIFSSG
                     KSLCKINNQTVTLQDLRKVMQELLDIHGQHETQSLLKQKYHLTLLDNYAESRYQDLLD
                     KYHQTFQNYKAKKQELEDLESADQALLQRLDLMKFQLEELSEAHLKEGEIEQLEIDIK
                     RIQNSEKLSLALNNAHMTLTDENAITDRLYELSNHLLTINDIVPNKYDKLKEDIDQFY
                     YILEDAKHELYDEMANTEFDEQVLNEYESRMNLLNNLKRKYGKDISELIAYQEKLNNE
                     INKIENYEQSTSQLREEINALYNQVIEVGQALSKQRRIVARELRDHIVSEIQNLQMKD
                     ANLEISFKKLEEPNIDGIEFVEFLISPNKGEPLKSLNKIASGGELSRIMLALKSIFVK
                     SRGQTAILFDEVDSGVSGQAAQKMAEKMRDIAEYIQVICISHLPQVASMSDHHLLISK
                     SSKDDRTTTQVQELISDDKVDEIARMISGASVTDLTRENAREMIQHNQRRR"
     gene            2452..3873
                     /gene="lpdA"
                     /locus_tag="O2701_002286"
     CDS             2452..3873
                     /gene="lpdA"
                     /locus_tag="O2701_002286"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCZ4294160.1"
                     /translation="MSEKQYDLVVLGGGTAGYVAAIRASQLGKKVAIVERQLLGGTCL
                     HKGCIPTKSLLKSAEVFQTVKQAAMFGVDVKDANVNFENMLARKEDIINQMYQGVKHL
                     MQHNHIDIYNGTGRILGTSIFSPQSGTISVEYEDGESDLLPNQFVLIATGSSPAELPF
                     LSFDHDKILSSDDILSLKTLPSSIGIIGGGVIGMEFASLMIDLGVDVTVIEAGERILP
                     TESKQASQLLKKSLSARGVKFYEGIKLSENDINVNEDGVTFEISSDIIKVDKVLLSIG
                     RKPNTSDIGLNNTKIKLSTSGHILTNEFQQTEDKHIYAAGDCIGKLQLAHVGSKEGVV
                     AVDHMFEGNPIPVNYNMMPKCIYSQPEIASIGLNIEQAKAEGMKVKSFKVPFKAIGKA
                     VIDSHDTNEGYSEMVIDQSTEEIVGINMIGPHVTELINEASLLQFMNGSALELGLTTH
                     AHPSISEVLMELGLKAESRAIHV"
     gene            3889..4881
                     /locus_tag="O2701_002287"
     CDS             3889..4881
                     /locus_tag="O2701_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HCZ4294161.1"
                     /translation="MIDYKSLGLSEEDLKVIYKWMDLGRKIDERLWLLNRAGKIPFVV
                     SGQGQEATQIGMAYALEEGDITAPYYRDLAFVTYMGISAYDTFLSAFGKKDDVNSGGK
                     QMPSHFSSRAKNILSQSSPVATQIPHAVGAALALKMDGKKKIATATVGEGSSNQGDFH
                     EGLNFAGVHKLPFVCVIINNKYAISVPDSLQYAAEKLSDRALGYGIHGEQVDGNDPLA
                     MYKAMKEARDRAISGQGSTLIEAVTSRMTAHSSDDDDQYRTKEERETLKKADCNEKFK
                     KELLSAGIIDDAWLAEIEAEHKDIINKATKAAEDAPYPSVEEAYAFVYEEGSLN"
     gene            4881..5864
                     /locus_tag="O2701_002288"
     CDS             4881..5864
                     /locus_tag="O2701_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCZ4294162.1"
                     /translation="MAKLSYLEAIRQAQDLALQQNKDVFILGEDVGRKGGVFGTTQGL
                     QQKYGEDRVIDTPLAESNIVGTAIGAAMVGKRPIAEIQFADFILPATNQIISEAAKMR
                     YRSNNDWQCPLTIRAPFGGGVHGGLYHSQSIESIFASSPGLTIVIPSTPYDAKGLLLS
                     SIESNDPVLYFEHKKAYRFLKEEVPEEYYTVPLGKADVKREGEDLTVFCYGLMVNYCL
                     QAADILAADGINVEVVDLRTVYPLDKETIIDRAKHTGKVLLVTEDNLEGSIMSEVSAI
                     IAEHCLFDLDAPIMRLAAPDVPSMPFSPVLENEIMMNPEKILNKMRELAEF"
     gene            5877..7151
                     /locus_tag="O2701_002289"
     CDS             5877..7151
                     /locus_tag="O2701_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HCZ4294163.1"
                     /translation="MEITMPKLGESVHEGTIEQWLVSVGDHIDEYEPLCEVITDKVTA
                     EVPSTISGTITEILVEAGQTVAIDTIICKIETADEKTNETTEEIQAKVDEHTQKSTKK
                     ASATVEQTSTAKQNQPRNNGRFSPVVFKLASEHDIDLSQVVGSGFEGRVTKKDIMSVI
                     ENGGTTAQSDKQVQTKSTSVDTSSNQSSEDNSENSTIPVNGVRKAIAQNMVNSVTEIP
                     HAWMMIEVDATNLVNTRNHYKNSFKNKEGYNLTFFAFFVKAVADALKAYPLLNSSWQG
                     NEIVLHKDINISIAVADENKLYVPVIKHADEKSIKGIAREINTLATKARNKQLTAEDM
                     QGGTFTVNNTGTFGSVSSMGIINHPQAAILQVESIIKKPVVINDMIAIRNMVNLCISI
                     DHRILDGLQTGKFMNHIKQRIEQYTLENTNIY"
     gene            7502..7939
                     /locus_tag="O2701_002290"
     CDS             7502..7939
                     /locus_tag="O2701_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HCZ4294164.1"
                     /translation="MDMNFDLYMNGVVEQARNEIESAGYEQLTTAEDVDKVLKQDGTT
                     LVMINSVCGCAGGIARPAASHALHYDVLPDRLVTVFAGQDKEATQRAREYFEGYAPSS
                     PSFALVKDGKITEMIERHQIEGHDVMNVINQLQTLFNKYCEER"
     gene            7953..8933
                     /locus_tag="O2701_002291"
     CDS             7953..8933
                     /locus_tag="O2701_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCZ4294165.1"
                     /translation="MLKLNPYKIGFRTIKTAVGMTLGVIISKLLGLDNYASSAILVVL
                     CIKHTKVHSLQAIISRLVSCFLVLFLGSAIFSLLGQSPIVLGIIVLLFIPLTVVLKVQ
                     EGVITSCVILLHVFNAKSIDAHLIVNETLLLLIGLSIAFTMNLMMPSLDKQLDEYKCK
                     IEQQIADIFSKYSYICEKYEDTIAIEFEVLLLNIKKAKSIAFRDVKNHFVRNENSYYH
                     YFDMREEQVELLMRMKPLIESICHKDPLLNDVASLLKEIGLNVNSNDYTAMRLHNLYE
                     TRLKLDELPLPTTHKALNSRASIIQILNELEEYLQIKSQFGSLKMHSEVA"
     gene            complement(9060..9239)
                     /gene="prli42"
                     /locus_tag="O2701_002292"
     CDS             complement(9060..9239)
                     /gene="prli42"
                     /locus_tag="O2701_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stressosome-associated protein Prli42"
                     /protein_id="HCZ4294166.1"
                     /translation="MLVISSQVKSFISYVVIINTKVIHLEAHKMQNKVLRIIIIVMLV
                     SVVLALLLTSIIPIL"
BASE COUNT         3449 a         1279 c         1777 g         2842 t
ORIGIN      
        1 caattgttat gcgtaatatg aaaaatattt ataaatagat aaccaagata gataaaccat
       61 gtttaccctt tttacgtaaa ggtgtttagt agtcgtaaat cagtgcatgt gttaaaataa
      121 atgtataaat attcgtaatc agaggtgttt actgtgccca aaaaatcggt taggcatata
      181 aaaattagag aaattatttc aaatgaacag atagagacac aagatgaatt agttaaacga
      241 ttaaacgatt atgatttaaa tgtcactcaa gcaactgttt ctcgtgatat taaagaacta
      301 caacttatta aagtacctat accttcaggt caatatgttt atagtttacc aaatgataga
      361 aaattccatc ctttagaaaa attgggacgt tatttaatgg attcctttgt taatatagat
      421 ggtactgata atttacttgt tctaaaaaca ttacctggta atgcacaatc tattggagct
      481 atattagacc aaatcaattg ggaagaagta ctaggcacaa tttgtggtga tgatacttgt
      541 ttaattattt gtcgaagcaa agaggcaagt gatgaaatca agtcaagaat tttcaatttg
      601 ttataaggat gcgatgagtt tatgttacaa accttatcaa tcaagcaatt tgctattatt
      661 gaagaattag aaattcaatt ttctgatggt ttaactgttt taagtggtga aacgggttca
      721 ggtaaatcaa ttattataga tgcaattgga caattaattg gaatgagagc ttcatctgat
      781 tttgtaagac atggtgaaaa aaaggctgtc atcgaaggta tatttgatat tgacgagagt
      841 aaagatgcta tacatatatt gaaaaatatg gatattgatg tagatgaaga ttttttatta
      901 gttaaacggg aaattttcag ctctggtaaa agtctttgta aaattaataa tcaaacagtt
      961 actttacaag atttacgaaa agttatgcaa gaattattag atattcatgg ccaacatgaa
     1021 acgcagtctt tgttgaaaca aaaataccat ttaacattat tggataatta cgcagaatca
     1081 cgttatcaag atttgttgga taaatatcac caaacttttc aaaattataa agccaaaaag
     1141 caagagttag aagatttaga atcagcagac caagcattgc tacagcgttt agatttaatg
     1201 aaattccaat tagaagaatt gtctgaagca catctaaaag aaggcgaaat agagcaactt
     1261 gaaatagata ttaaacgcat tcaaaactct gaaaaattaa gcttagcgct taataacgca
     1321 catatgacat taacggatga aaatgctatc actgatcgtt tatatgaact aagcaaccat
     1381 ttgttaacaa taaacgatat agttccaaac aaatatgaca aattaaaaga agatattgat
     1441 caattttact acattttaga agatgcaaaa catgagttat atgatgaaat ggctaataca
     1501 gaattcgacg aacaagtgtt aaacgagtat gaatctcgta tgaatttgct gaataattta
     1561 aaacgtaaat atggaaaaga tatttcagaa ttaatcgcat atcaagaaaa gcttaataat
     1621 gaaatcaata aaattgaaaa ctatgaacaa agtacatcgc agttacgaga agaaataaat
     1681 gcattgtata atcaagttat agaggttgga caagcgttat caaagcagcg tcgtatagtc
     1741 gctagggagt taagagacca cattgtatcg gaaattcaaa acttacaaat gaaagacgca
     1801 aatcttgaga tttcatttaa aaaattagaa gaaccgaata ttgatggaat cgaatttgta
     1861 gaatttttaa tcagtccaaa taaaggggaa ccattaaaaa gtttaaataa aattgcgtca
     1921 ggtggagaac tttctagaat tatgttagcg ttaaaaagta tttttgttaa atcgagaggt
     1981 caaactgcaa ttttattcga tgaggttgac tcaggtgtat ctggacaagc tgcacaaaaa
     2041 atggcagaga aaatgcgaga tatagcagaa tatatacaag ttatttgtat ctctcactta
     2101 ccacaagttg catcgatgag cgaccatcat ttattaattt cgaaatcatc aaaagatgat
     2161 cgtacaacaa cacaagttca agagttaatt agcgatgata aagtagatga aattgcacga
     2221 atgatttcag gtgcaagtgt tactgattta actcgagaaa atgcaagaga aatgatacaa
     2281 cacaatcaaa gacgtagata atttccaaag tgtgttgaat actcatttat ggatttattt
     2341 atgtgcattc aaactaaata gacaatcatg taaaataggt aattaatgga catgaaaact
     2401 atgtattaat aaattttcaa tggatatata tcattagtta ggagtaagaa catgtcagag
     2461 aaacaatatg atttagtcgt tctcggtgga ggtactgcag gatatgttgc tgcgatacgt
     2521 gcgtctcaat taggtaaaaa agtggcaata gtggaacgac aactattagg aggtacttgt
     2581 ctccataaag gttgtattcc tactaaatcg ctgcttaaat ctgctgaagt atttcaaaca
     2641 gtaaaacagg cagcaatgtt tggcgttgat gtcaaagatg ctaatgttaa ttttgaaaat
     2701 atgttggcac gaaaagaaga cattattaat caaatgtatc aaggtgtaaa gcatttaatg
     2761 caacacaatc acattgacat ttataatggc acgggacgta ttctaggtac atctatattt
     2821 tcacctcaaa gtggcacaat ttctgtcgaa tatgaagatg gtgaatcaga cttattaccc
     2881 aaccaatttg tattaattgc aactggttct tcgcctgcag agttgccatt cttatcattt
     2941 gatcatgata aaattttatc tagcgatgat atattatcat taaagacgtt accatcaagt
     3001 attggtatta ttggtggtgg tgttatcgga atggaatttg catcattgat gatagattta
     3061 ggtgttgacg taacagttat agaagcaggt gaaagaattt taccaactga aagtaaacaa
     3121 gcttcacaac tattaaaaaa atcattgtca gcacgtggtg ttaaattcta tgagggaata
     3181 aaactttctg aaaatgatat aaatgttaac gaagatggtg ttacgtttga aatttcctca
     3241 gacataatta aagtagataa agtgttgcta tcgattggta gaaaacctaa cacatcagat
     3301 attggtttaa acaacactaa aataaaactt tcaacatcag gacatatttt aacgaacgaa
     3361 tttcaacaaa ctgaagataa acatatttat gcagctggtg attgtatagg aaaattacaa
     3421 ttggcacatg ttggatcaaa agaaggtgtt gtggctgttg atcatatgtt tgaggggaac
     3481 ccaatcccag taaactataa catgatgcct aagtgtattt attcacaacc tgaaattgct
     3541 tctatcggtt taaacattga acaagcaaag gcagagggaa tgaaagttaa aagttttaaa
     3601 gtaccattta aagcaattgg taaagcagtg attgatagcc atgacacaaa cgaagggtat
     3661 agcgaaatgg tgattgatca atcaactgaa gaaattgtgg gtattaatat gattggtcca
     3721 catgtaacag aattgattaa tgaggcatca ctgttacagt tcatgaatgg ctcggcatta
     3781 gaattaggac taacaacaca cgcacatcct tccatctctg aagtgttgat ggaattagga
     3841 ttgaaagcag aaagtagagc tattcacgta taaataggag gatttaacat gattgattat
     3901 aaatcattag gccttagcga agaagaccta aaagtaatat ataaatggat ggatttaggt
     3961 agaaaaatag atgaaagact gtggttattg aaccgtgcag gtaaaattcc atttgttgta
     4021 agtggtcaag gacaagaagc aactcagatt gggatggcat acgcattaga agaaggtgat
     4081 attacagcgc cttattatag agatctagct ttcgttactt atatgggtat ctcagcgtat
     4141 gatacgtttt tatcagcgtt tggaaagaaa gacgatgtta actctggtgg aaaacaaatg
     4201 ccatcacact ttagtagtag agctaagaat attttgtctc aaagttcacc ggtagcaact
     4261 caaatacccc atgctgttgg tgctgcttta gctttaaaaa tggatggtaa gaagaaaatt
     4321 gcaacagcaa ctgttggtga aggtagctca aaccaaggtg acttccatga aggtttaaac
     4381 ttcgcgggtg tacacaaact tccttttgta tgtgtcatta taaataataa atatgcaatt
     4441 tcagtacctg attcattaca atacgcagct gaaaaattat cggatagagc acttggttac
     4501 ggtatacatg gtgaacaagt agatggtaat gatccattag caatgtataa agcgatgaaa
     4561 gaagcaagag atcgagctat ttctggtcaa ggttcaactt tgattgaagc agtaacaagt
     4621 cgtatgacag ctcattcatc agatgatgac gaccaatatc gtacgaaaga agagcgtgaa
     4681 acgcttaaaa aagcagactg caatgaaaag ttcaaaaaag agttgctttc agctggcatt
     4741 atcgatgatg cttggttggc agaaatagaa gcggagcata aagacatcat caataaagcg
     4801 acaaaggctg ctgaagacgc gccatatcca agtgtcgaag aagcctatgc atttgtgtac
     4861 gaagaaggga gtcttaacta atggctaaat tatcttattt agaggcgata cgccaggcac
     4921 aagacttagc attacaacaa aataaagatg tgtttattct tggtgaagat gttggtagaa
     4981 aaggtggcgt atttggtact actcaaggac tgcaacaaaa atatggtgaa gacagagtta
     5041 tcgatacgcc attagctgag tcaaatattg ttggaactgc cattggtgca gctatggtag
     5101 gtaaaagacc aattgcagaa attcaatttg cagattttat tttacctgcg acaaatcaaa
     5161 ttattagtga agctgcgaaa atgcgttatc gttcaaataa tgattggcaa tgcccattaa
     5221 cgattcgtgc accatttggt ggcggtgttc acggaggact atatcattca caaagtattg
     5281 aaagtatatt tgcttcatcg ccaggtttaa caattgttat tccttccaca ccgtatgatg
     5341 ctaaagggct tttattatca tctatcgaat caaatgatcc agtattgtac tttgagcata
     5401 aaaaagctta tcgtttctta aaggaagaag tgccagaaga atattacact gtacctttag
     5461 gaaaagcaga tgtgaaacgt gaaggtgaag atttaacagt attttgttat ggattaatgg
     5521 tcaattattg tttacaagca gcggatattt tagctgcaga cggtatcaat gttgaagtcg
     5581 ttgatttacg aactgtttat ccattagata aagaaacaat tattgatcgt gctaaacata
     5641 caggtaaagt tttacttgta actgaagata atttagaagg tagtattatg tcagaagtgt
     5701 cagcgattat tgcagagcat tgcttgttcg atttagatgc accaatcatg cgtttagctg
     5761 ctccagatgt accatctatg ccattttctc ctgtattaga aaatgaaatt atgatgaatc
     5821 cagaaaaaat cttaaataaa atgcgtgaat tagcagaatt ctagggaggg aaagtcatgg
     5881 aaataacaat gcctaagtta ggtgagagtg ttcatgaagg caccattgaa caatggttag
     5941 tttctgttgg tgatcatatt gatgaatatg aaccattatg tgaagttatt acagataaag
     6001 tgacagctga agtcccttcc acgatatcag gaacaattac agaaatttta gttgaagcgg
     6061 ggcagacagt agctattgat acaattatct gtaaaattga aactgctgat gaaaagacaa
     6121 atgaaacaac tgaagagata caagcaaaag tggatgagca tactcagaaa tctactaaaa
     6181 aagctagtgc aacagtggaa cagacatcta ctgctaaaca aaatcaacca cgtaataatg
     6241 gtcgcttttc acctgttgta tttaaactcg cttcagagca tgacattgat ttatcacaag
     6301 ttgtaggtag tggatttgaa ggtcgtgtaa ctaagaagga tataatgtca gttattgaaa
     6361 atggtggtac cacagctcaa tctgacaaac aagttcaaac aaaatcaaca tcagtagata
     6421 catcaagtaa ccaatcatct gaagacaata gtgaaaacag cacaatacca gtaaatggtg
     6481 tgcgtaaagc aattgcgcaa aatatggtta atagtgtaac agagattcca catgcatgga
     6541 tgatgattga agtagatgct acaaatcttg tgaatacgag aaatcattat aaaaacagct
     6601 ttaaaaataa agaaggatat aatctaacgt tctttgcttt ctttgtaaaa gctgtagcag
     6661 atgctttaaa agcatatcct ttattaaata gtagctggca aggaaatgaa attgtcttac
     6721 ataaagacat taatatttca attgctgttg ctgatgaaaa taaattatac gtacctgtga
     6781 ttaagcatgc agacgaaaag tcaatcaaag gtatagctag agaaattaat actttagcaa
     6841 cgaaagcgcg taataagcaa ttgacagctg aagatatgca gggcggtaca tttacggtaa
     6901 ataatactgg tacatttggt tcagtatcat caatgggtat tataaatcat ccacaagcag
     6961 cgattttaca agtagaatca atcattaaaa agccagtagt aattaatgat atgattgcaa
     7021 ttcgtaacat ggttaattta tgtatttcaa ttgatcatcg tattttagat ggtttacaaa
     7081 caggtaaatt tatgaatcat attaaacagc gtatcgaaca gtatacttta gaaaatacaa
     7141 atatatatta gtgataacat agatgcatct atcgacaact tgttttatct tgttcttgtc
     7201 gatggatgta ttattttttg gcactaaaat atgtgcaata tattcaaaaa agataaagaa
     7261 caataatcaa catggttgaa tgcatttttg cagtaaagtc aaaataagac atcatacttg
     7321 aaacatatta atgaaaacat gtgaacaaat tagttaccat gattttaagc acaataatgt
     7381 ttggtatatt gttaaaattg tgtctaaata taggtgtgat tcagattagt ttattgaaca
     7441 atatgttatt aattagtaga atgaggatag tttaaatata aagggatagg tgattgaact
     7501 tatggacatg aatttcgatt tatacatgaa cggtgttgta gaacaagcaa ggaatgaaat
     7561 tgaatctgcg ggatatgagc aattaactac tgcagaagat gttgacaaag ttcttaaaca
     7621 agatggtaca acactagtta tgatcaattc tgtatgtggt tgtgcaggtg gtatcgcaag
     7681 accagcagca tcacatgctt tacattatga cgtattacct gatcgtctag tgacagtatt
     7741 tgctggacaa gataaagaag cgacacaaag agcgcgtgaa tacttcgaag gttatgcgcc
     7801 ttcaagtccg tcatttgcat tagtaaaaga tggaaagatt acagaaatga ttgaaagaca
     7861 tcaaatcgaa ggtcatgatg tgatgaacgt aattaatcaa ttacaaacat tattcaataa
     7921 atattgtgaa gaaagataag aggcgctaac ccatgttaaa gttaaatcct tacaagattg
     7981 gatttagaac aataaaaaca gcagtgggta tgactttagg tgtaattatt agtaagctgt
     8041 taggtttaga taattatgct tcaagcgcca tattagtcgt attatgtatt aaacatacaa
     8101 aagtacattc gctacaagcg attatttcaa gattagtatc atgtttttta gtattgtttt
     8161 taggttcagc aatatttagt ttattaggtc agagtccaat tgtactcggt attatcgtat
     8221 tgttatttat accattaact gtcgtattaa aagtacaaga aggtgtcatt acgagttgcg
     8281 ttatattact tcatgttttt aatgcaaaat caattgatgc acatttaatt gttaatgaaa
     8341 cattattact gttaattgga ctaagcattg catttacaat gaatttaatg atgccaagtt
     8401 tagacaaaca actagacgaa tacaaatgta aaattgagca acaaattgct gatattttta
     8461 gtaaatatag ttatatttgt gaaaaatatg aagataccat tgcgattgaa tttgaagtgt
     8521 tacttttaaa tattaaaaag gcgaagtcta tcgcgttccg agatgttaaa aatcattttg
     8581 ttagaaacga aaattcatac tatcattatt ttgatatgcg agaagagcaa gtggaattgt
     8641 taatgagaat gaaaccgctc atcgaaagta tctgtcataa agatccgctt ttaaatgatg
     8701 ttgcgtcact gctgaaagaa attggtctaa atgtgaatag taatgactat acagcaatgc
     8761 gtttacacaa tttgtatgag acgcgtttga aattggatga actgccattg ccaacaacac
     8821 acaaagcgct taactcgaga gcaagtatta ttcaaatatt aaatgaatta gaagaatatt
     8881 tacaaatcaa atcgcaattt ggttcattga aaatgcatag tgaagtagct taaaaaataa
     8941 tccatccgta tgagccatgt ttgaaagtaa tcgttgcaat acgataactt attaatacat
     9001 gcgtttataa tggatggatt tttaattgta tgattttgta taggtagttg agatatagtt
     9061 tataaaattg gaatgatact cgttaataac aatgctaata caactgatac aagcataacg
     9121 ataatgataa ttcttaaaac tttattttgc attttgtgtg cctccaaatg aataaccttt
     9181 gtattaataa ttacgacata agatataaaa cttttaactt gagacgatat aactagcaat
     9241 catcataaag gaatataata aaaaacatga gtaagtttgc ttatgtcatt ttcctcatta
     9301 tacgatatct ttgaaaaatc aatcaatata tttttgacaa agcttag
//
LOCUS       DALAGD010000103         9338 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.104, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000103 DALAGD010000000
VERSION     DALAGD010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9338
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.104"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(226..726)
                     /locus_tag="O2701_002293"
     CDS             complement(226..726)
                     /locus_tag="O2701_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCZ4294167.1"
                     /translation="MKKLVTATTLTAGIGTALVGQAHHADAAENYTNYNNYNYNTTQT
                     TTTTTTTTTTSSISHSGNLYTAGQCTWYVYDKVGGEIGSTWGNANNWAAAAQGAGFTV
                     NHTPSKGAILQSSEGPFGHVAYVESVNSDGSVTISEMNYSGGPFSVSSRTISASEAGN
                     YNYIHI"
     gene            complement(963..1385)
                     /locus_tag="O2701_002294"
     CDS             complement(963..1385)
                     /locus_tag="O2701_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HCZ4294168.1"
                     /translation="MTTEMNDVTNHQPNRKSNDENIMAMLIYLLSLFTSIIGPLIIWL
                     LKKDESKLVDRAGKNYLNYTISYIIWSIVLVVITLIGVFLIATDIDFIIIIGFIITFI
                     GILSIFAFSILSFVFTIIACVKYYNGQEYIIPLTIRFI"
     gene            1633..2715
                     /locus_tag="O2701_002295"
     CDS             1633..2715
                     /locus_tag="O2701_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HCZ4294169.1"
                     /translation="MKIAIVGSGNGAVTAAVDMVSKGHDVKLYCRNQSISKFQNAIEK
                     GGFDFNNEGDERFVKFTDISDDMEYVLKDAEIVQVIIPSSYIEYYADVMAEHVTDNQL
                     IFFNMAAAMGSIRFMNVLEDRHIETKPQLAEANTLTYGTRVDFENAAVDLSLNVRRIF
                     FSTYDRSCLNDCYDKVSSIYDHLVKEESLIKTNLENGNPEVHPGPTLLNVGRIDYAGE
                     FALYKEGITKHTVRLLHAIELERLNLGRRLGFELSTAKESRIERGYLERDKEDEPLNR
                     LFNTSPVFSQIPGPNHVESRYLTEDIAYGLVLWSSLGRVIDVPTPNIDAVIVIASTIL
                     ERDFFEEGLTVEEIGLDKLDLEKYLK"
     gene            2810..4210
                     /gene="nhaC"
                     /locus_tag="O2701_002296"
     CDS             2810..4210
                     /gene="nhaC"
                     /locus_tag="O2701_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCZ4294170.1"
                     /translation="MMKRKPTFLESISTMIVMVIVVVTGFVFFDIPIQVLLIIASAYA
                     TWIAKRVGLTWQDLEKGIAERLNTAMPAILIILAVGIIVGSWMFSGTVPALIYYGLDL
                     LNPSYFLISAFFISAVTSVATGTAWGSASTAGIALISIGNQLGIPPGMAAGAIIAGAV
                     FGDKMSPLSDTTNLAALVTKVNIFKHIHSMMWTTIPASIIGLLVWFIAGFQFKGHSND
                     KQIQTLLSELAQIYQINIWVWVPLIVIIVCLLFKMATVPAMLISSFSAIIVGTFNHHF
                     KMTDGFKATFSGFNESMIHQSHISSSVKSLLEQGGMMSMTQILVTIFCGYAFAGIVEK
                     AGCLEVLLTTISKGIHSVGSLICITVICCIALVFAAGVASIVIIMVGVLMKDLFEKYQ
                     VSRSVLSRTLEDSSTMVLPLIPWGTSGIYYTNQLHVSVEEFFIWTVPCYLCAIIAIIY
                     GFTGIGIKKSSNSRLT"
     gene            4384..4626
                     /locus_tag="O2701_002297"
     CDS             4384..4626
                     /locus_tag="O2701_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294171.1"
                     /translation="MTKLIENLKASIFVSTLTKNLSMYSRFDMPKFGCVNGCMLFILY
                     YRVNRIGENRNFEIFTNSIFFIEIKKANKALETLLA"
     gene            complement(4643..5446)
                     /locus_tag="O2701_002298"
     CDS             complement(4643..5446)
                     /locus_tag="O2701_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCZ4294172.1"
                     /translation="MKKIATATIATAGFATIAIASGNQAHASEQDNYGYNPNDPTSYS
                     YTYTIDAQGNYHYTWKGNWHPSQLNQDNGYYSYYYYNGYNNYNNYNNGYSYNNYSRYN
                     NYSNNNQSYNYNNYNSYNTNSYRTGGLGASYSTSSNNVQVTTTMAPSSNGRSISSGYT
                     SGRNLYTSGQCTYYVFDRVGGKIGSTWGNASNWANAAARAGYTVNNTPKAGAIMQTTQ
                     GAYGHVAYVESVNSNGSVRVSEMNYGYGPGVVTSRTISASQAAGYNFIH"
     gene            6056..8011
                     /locus_tag="O2701_002299"
     CDS             6056..8011
                     /locus_tag="O2701_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ4294173.1"
                     /translation="MTDNYSLHIYKSMKMSSVKEANLKIIYWLQGTGVVSINLQQYDL
                     KRNYVTIIMLNDLYQIDGNDDSVCCVVEVSAKTFLKFMNANYMMRGKILTNEVASTFR
                     ILIKYLIYSKIKQQSYNANDKIINYMCVELMSLNTYDGSRHLVAEEVHDYLTNNHHKK
                     INRKDVINQVSITNKALSDMFKATPYSNFIQYLNHIRLEHCLIDILTSKKPIEEIASS
                     HGFNHYSRFIHLFKETYGNTPKLIRKRYSPTSHSINHSQLVEIDKNIIELFDDTNQSS
                     SSMREIDIPFEPTKRSQMYQPKNIYIKGSNFYWMDYYTIKQIIQRLGVNPENLHIIVT
                     IDTSNELSIEQITLLLQKIVNYNVNVVFRIKHEYKELLTSAERGKIEKLVATIFNMTF
                     ESNRCKIAFLINDLNTTAIQKLKRLIDNYIGVFELIYHLEPKELNTRYDKEIDHLIDY
                     FILPLRQIEQVSIARSKIICDVDLINENNDLKEKITMSDINKVSELLTNYTKFRGLIM
                     HMSIISESSKEINLTRLSIFIFIINILNQLRGIVIYQNDTMIVTKFKHEVQCVICLPA
                     ALLNENSDAIRLSCKGLQQFSHAKTKQIVLSIDKDNHTSENGVLVNDIMQQHSYWKAN
                     FIERNIYDHSLMVSSNSIVHLKYHLEI"
     gene            8037..8780
                     /locus_tag="O2701_002300"
     CDS             8037..8780
                     /locus_tag="O2701_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HCZ4294174.1"
                     /translation="MSKFKVKRLTAHILMLKVINDMLKYSFHLTLTQVKLLNLLVKYD
                     NSDSNSASIDSLLKMKEIQSKMALLQMLSYLNNHQWLLKGRDQRDQRKLTISINKIHI
                     DKIEYMNNELSDYIERYFGQYTFDFSCDYVSYLLSSQELLRNLKCYLNMCQLSLEELY
                     VLGILNLHKGQLTVKELQGEFHHPIFAVSPILKCLILKGLVKKERCELDERRVIVTIK
                     REKFSKVTMLIQACYNYLEKGIQVNLNNK"
     gene            8844..9170
                     /locus_tag="O2701_002301"
     CDS             8844..9170
                     /locus_tag="O2701_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCZ4294175.1"
                     /translation="MILDNVNPNDLFPTEKKGPSVLGIIEYQVQGENEFEGAFIATNE
                     RLIMNVDMNGQFYYRSISYNEIEKIDYDGQTIMFKFNIGNVPMHDIKSANVEMFVEYV
                     KQHMIV"
BASE COUNT         3265 a         1200 c         1668 g         3205 t
ORIGIN      
        1 tattgtaatg tggaaaaact ggatcaatta aattagataa cgtagtttta aagttaatag
       61 tattagaaaa aattaatatt ttgaatatgg gaggaaatat aaataagtag gtggcaacga
      121 aaaatagcaa aaaaagagct tctcctataa aggaaagctc aaagtttttt gatgacatat
      181 gtactagaat taagtttcaa gacaatatgt atcatcgtgt ttatattaaa tatggatgta
      241 gttgtagtta cctgcttcac ttgcagaaat agttctagaa cttactgaga aaggtccgcc
      301 actataattc atttctgaaa ttgtaactga accatcactg tttacacttt ctacatatgc
      361 aacgtgacca aatggtcctt cagaagattg taggatagcg cctttagaag gtgtatgatt
      421 tactgtgaat ccagcacctt gtgcagcagc agcccaatta ttagcatttc cccaagtaga
      481 accgatttct ccgccaactt tatcatatac ataccaagta cattgtcctg cagtgtataa
      541 gttaccagaa tgtgaaattg atgatgtagt tgtcgtagtt gtcgtagtcg ttgtagtttg
      601 agtcgtgttg tagttatagt tgttgtaatt tgtataattt tcagcagcat ctgcatgatg
      661 tgcttgacct actaatgctg tgccgattcc tgctgttaac gtagttgctg ttactaattt
      721 tttcatgaat aaagtcctcc aaagttctat atcttttttt ataaataaaa cgtagcgact
      781 gttttattct cacatctcga attgatgaca atagttactt taacaaaatt aatgcttctt
      841 gtggggaatg ttattgattt gtaaaagaat aaaaaaactt tgactaattt tgtaataaaa
      901 attagtcaaa gttacaatga gattaacaga taattaatag gaaatattta tttgtaatat
      961 gtttaaataa atcgaattgt taaaggtatt atatattctt ggccattata atatttgaca
     1021 cacgcaataa ttgtgaatac aaaagataat attgagaaag cgaatatgga taaaataccg
     1081 ataaacgtaa tgatgaaacc tataataata atgaaatcaa tatctgtagc aattaggaaa
     1141 acgcctatta aagtgataac gactaaaacg atagaccaaa taatataaga aatcgtatag
     1201 ttaagataat tttttccagc acgatcaact agtttcgatt catctttttt caataaccat
     1261 attatcagtg gaccaataat agatgtgaat aaacttaata aatagataag catcgccata
     1321 atgttctcat cattggattt gcgattcggt tgatgatttg ttacgtcgtt catttcagtt
     1381 gtcatattag acactccttt gaaaattgta atattatctt taactataac aaaatataat
     1441 caaaaataaa catgtttatt aaacaattat taaaaataaa aataattggt ggacgtcggc
     1501 gtttaaatag gttaatttaa ggttatatat acttaacatt tataatgatg cgtaatgaat
     1561 tcgcatcatt tttatattgt cttacgtata atttgttttt aattttaacc aaagatagaa
     1621 agagggttgt ttatgaaaat agcaattgta ggatcaggaa atggcgcagt tacggcagca
     1681 gtagatatgg tgagcaaagg ccacgatgtt aaattatatt gtcgtaatca atctataagt
     1741 aagtttcaaa acgcaatcga aaagggcgga tttgatttta ataatgaagg tgatgaacgt
     1801 ttcgtaaaat tcactgatat tagtgatgat atggaatatg ttttaaaaga tgctgaaatt
     1861 gttcaagtga ttattccatc ttcatacata gagtattatg ctgatgtaat ggcagagcat
     1921 gtaactgata atcagttgat attcttcaac atggctgcag caatggggtc aattcgtttt
     1981 atgaatgttt tagaagatag acatattgaa acaaaaccac aactagcgga agctaatacg
     2041 ttgacgtatg gtacgcgtgt cgattttgaa aatgcagcag ttgatttatc tctaaatgta
     2101 cgtcgtatct tcttttcaac atatgataga agctgtctaa atgattgtta tgacaaagtt
     2161 tcaagtattt atgatcattt agtaaaagag gaaagcttaa ttaaaacaaa tcttgaaaat
     2221 ggtaatcctg aagtgcatcc aggaccaaca ttattgaatg tcggtcgcat tgattatgct
     2281 ggcgagttcg ctttatataa agaaggaatt actaaacata cagttagatt acttcatgca
     2341 atcgaattag aacgtttgaa tttaggccgt agattaggtt ttgaattatc aacagctaaa
     2401 gaatcacgta ttgaacgtgg ttatttagaa cgtgataaag aagatgaacc attaaatcgt
     2461 ttgtttaata caagcccagt attttcacaa attccaggac caaatcatgt agaaagcaga
     2521 tatttaactg aagatattgc atatggttta gtactatggt caagcttagg tcgtgttatt
     2581 gatgtaccga caccaaatat agatgcagta attgtaattg catcaaccat tttagagaga
     2641 gacttctttg aggaaggctt aacagttgaa gaaattggtt tagataagct tgatttagaa
     2701 aaatatttaa aataaatgat ggcttgaaga tagaaaagga tatagcatta tgcaaaagca
     2761 ataaattgaa gaaaagaggt ttctcatcaa taagcgaagg ggacgataga tgatgaaaag
     2821 aaaacccacc tttttagaat caatttcgac aatgattgta atggttattg ttgttgtaac
     2881 aggctttgtg ttttttgata ttccaattca agtattatta attattgcct cagcatatgc
     2941 cacatggatt gcaaaacgtg taggcttaac atggcaagat ttagaaaaag gcattgcaga
     3001 acgtttaaat actgcaatgc ctgcaatttt aattatacta gcggtaggaa ttatagtagg
     3061 cagttggatg ttttctggca cagtgccagc cttgatttat tatggcttag atttattgaa
     3121 tccaagctat tttttaatat cagccttttt tataagtgct gttacatctg tagcaactgg
     3181 tacagcatgg ggctctgcat caactgcagg gattgcactt atttctattg gtaatcaatt
     3241 ggggattcct ccagggatgg cagcgggtgc tattatagca ggggctgtgt ttggcgataa
     3301 aatgtcacca ttatcagata caactaattt agcggcgctt gttactaaag ttaatatatt
     3361 taaacatata cattcgatga tgtggacgac gatacctgca tcaatcatag gtttattagt
     3421 atggtttatt gctggatttc aatttaaagg gcattcaaat gataaacaga ttcaaacttt
     3481 gttatcagag cttgcacaga tttatcaaat taacatatgg gtctgggttc ccttaattgt
     3541 gatcattgtt tgtttgctat ttaaaatggc tacagtgcca gctatgctaa tatcaagctt
     3601 ttctgccatt atagtgggga cttttaatca tcatttcaaa atgacagatg gtttcaaagc
     3661 aacatttagt ggttttaacg aatcaatgat acatcagtct catatttcat ccagtgtgaa
     3721 aagcttgtta gaacagggtg gtatgatgag tatgacccaa atattagtaa cgatattttg
     3781 cggatatgca tttgcaggta ttgtagaaaa agcaggatgt ttagaagtct tattaactac
     3841 tatttctaaa ggcatccatt ctgtaggaag tttaatatgt attactgtta tttgttgtat
     3901 tgcgcttgta ttcgctgcag gtgttgcttc gattgtaatt attatggtcg gtgtgttaat
     3961 gaaagatttg ttcgaaaaat accaagtctc tcgttccgtt ttatcaagaa cacttgaaga
     4021 ctcaagtacg atggtcttac ctttaatacc atggggaaca tcaggtattt actatacgaa
     4081 tcaacttcat gtctctgttg aagaattttt catatggaca gtaccatgtt atttatgcgc
     4141 aattatagca attatctatg gttttacagg gataggtatt aaaaagtcat cgaattcacg
     4201 tttaacttaa tgtgagcgtg gaatatatat aatatgttga aacactttaa tcatttataa
     4261 ttgtagcggt tataatttga aaaggtttta acttagaata aatatcctct atacatatac
     4321 tgaatatgtt ttgtagcgga acatgttgat atatgtaatg taagttttat gtcatgattt
     4381 gtaatgacta aattaattga gaatttgaag gcaagtatat ttgtaagtac tttaactaaa
     4441 aatttatcaa tgtatagccg atttgacatg cctaaatttg ggtgtgtcaa tggctgtatg
     4501 ttgtttattc tttattacag agtgaatcgg attggtgaaa atcgaaattt tgagattttt
     4561 accaattcga tttttttcat agaaattaaa aaagccaaca aggctcttga aaccttgttg
     4621 gcgtaaacat agccatcact aattagtgaa tgaagttata accagcagct tggctagctg
     4681 agattgtacg tgaagttaca acacctgggc cataaccata gttcatttct gaaactctta
     4741 ctgaaccatt gctgttaaca ctttcaacgt atgcaacgtg accgtatgca ccttgagttg
     4801 tttgcataat tgcaccagct tttggtgtat tgttcactgt gtaaccagct cttgcagctg
     4861 cgttagccca gttacttgca ttgccccaag ttgaaccgat tttaccacct acacgatcaa
     4921 atacgtagta tgtacattga ccagaagtgt ataagttacg tcctgaagta taaccacttg
     4981 agattgaacg gccatttgat gatggagcca tagttgtagt tacttgaaca ttgttgcttg
     5041 aagtgctgta gcttgcacct aaaccaccag tacggtagct gtttgtgttg taactattat
     5101 agttattgta gttatatgat tgattattat ttgagtagtt gttgtaacgg ctgtagttat
     5161 tgtagctata accgttgttg taattgttat agttattgta accattgtag tagtaatagc
     5221 tgtagtagcc attatcttgg tttaattgac ttggatgcca gttacctttc catgtgtaat
     5281 ggtagttacc ttgtgcatca atagtgtaag tatagctata tgatgttggg tcgtttggat
     5341 tataaccgta gttatcttgc tcagaagcat gagcttgatt tcctgatgca attgcgattg
     5401 tagcgaatcc tgcagttgcg atagtagctg tagcgatttt cttcatttta aaaatatcct
     5461 cctaaaaatt ttaaatctaa aatattttcg taatgtccgt gtgacaaaat taatgttata
     5521 agttatctct cgtaattaaa cgacaagaaa gactataaca gaaattagcg tccttgtgtg
     5581 ctttgttaac gttttgtaat tttttgctaa tatcttgaca caatagaatt ttaaaagtat
     5641 agaaatttgc attttgcaaa acttataact acggcattct ttgtgaaaac tgaatgtttc
     5701 gaaaataagt ctgttacaaa tttgtaatat tactgaaaat tctaaatgta tattttgtgc
     5761 ataatatagg acttttaatc agaaaatgca taaaaattcg acacgaacga tcaagtttct
     5821 atgattatga aagctttggt atttggatga aaatgtatat ttaatggata aaatatccta
     5881 atttagcata aaaaaatgtt ttaataaaag tattatttga tataatcgat ttatgttttg
     5941 ttactgctaa aaaacatgtg gcgatataat tggtagtgaa tattagatgt tgacaattga
     6001 tttgagaaag acaaacagga gtaaatcaat atatttgtat atgtgaggat gtaagatgac
     6061 agataattat tcactacata tttataaaag tatgaaaatg agtagtgtga aggaagcgaa
     6121 cttaaaaatt atatattggt tacaaggtac aggtgtagtt tcaatcaatc tacaacaata
     6181 tgatctaaaa agaaattatg tcaccattat tatgttgaat gatttgtatc aaattgatgg
     6241 taatgatgat agtgtatgtt gtgttgttga agtaagtgca aagacatttt tgaagtttat
     6301 gaatgcaaac tatatgatgc gtggcaagat attaacaaat gaggttgcta gcacttttag
     6361 aatcttgatt aagtatttaa tctacagcaa aattaaacaa cagtcttata atgctaatga
     6421 taagattatt aattacatgt gtgttgaatt aatgtcatta aatacatacg atggtagtcg
     6481 tcatttagta gccgaagaag tgcacgatta tttaacaaac aaccaccata aaaagataaa
     6541 taggaaagat gtcattaatc aagttagtat tacaaacaaa gctttaagtg acatgtttaa
     6601 agcgacacct tatagtaatt ttattcaata tttaaatcat attagactgg agcattgctt
     6661 aattgatatt ttaacttcta agaagcctat tgaagaaatc gcaagtagtc atggttttaa
     6721 tcattattct cgttttatac acctctttaa agaaacgtat ggtaacactc ctaaattaat
     6781 tagaaaaaga tatagtccaa catctcattc aattaaccat tcccaattag ttgaaattga
     6841 taaaaatata attgaattat ttgatgatac taatcaaagt tcttcaagta tgcgcgagat
     6901 tgatatacct tttgagccta caaaaaggag tcaaatgtat caacctaaaa acatttatat
     6961 taaaggaagt aatttttatt ggatggatta ttatactatt aaacaaatca ttcaaagatt
     7021 aggtgttaat cctgaaaact tgcatataat tgttacaatc gacacaagca atgagctatc
     7081 gattgaacaa atcactttat tacttcaaaa gatagtgaat tacaatgtaa atgtagtgtt
     7141 tcgtattaaa catgaatata aggaattgtt aacgagtgct gaaagaggca agatagaaaa
     7201 gctagtagct acaattttta atatgacttt tgagagtaat agatgtaaga ttgcattttt
     7261 aattaacgac ttaaatacta ctgcaattca aaaactgaaa cgtttaatag ataattatat
     7321 aggagtattt gaattaatat atcatctgga accaaaagag ttgaatacgc gctatgataa
     7381 agaaattgat catctaatag actattttat cttaccgcta agacaaatag aacaggtgtc
     7441 tattgcacgt tctaaaatta tatgtgatgt agatttgatt aatgagaata atgacttaaa
     7501 agaaaaaatc acaatgtctg atattaataa ggtttctgaa cttttgacaa attatacaaa
     7561 atttcgagga cttattatgc acatgtcaat tataagtgaa tcgagcaaag aaataaattt
     7621 aactagatta tcaatattca tttttatcat aaatatactt aatcaattac gagggattgt
     7681 tatttatcaa aatgacacga tgattgtaac gaagttcaaa catgaagtac aatgtgtcat
     7741 ttgtttgcca gccgcattgt taaatgagaa ttcagacgct atacgtttat cgtgtaaggg
     7801 tcttcaacaa tttagccatg ctaaaactaa acaaattgtt ttaagtatag ataaagataa
     7861 tcatacttcc gaaaatggtg tacttgtgaa tgatattatg caacaacaca gttattggaa
     7921 agctaatttt atagaaagaa atatttatga tcattcatta atggtctcaa gcaatagtat
     7981 tgttcatttg aaatatcatc ttgagatata ggcatggttt aagagaggtt ttaaagatgt
     8041 ctaaatttaa agttaagcgt ttaactgcac acatactaat gttgaaagtc attaatgata
     8101 tgttgaaata ttcatttcat ctcactttga ctcaagtgaa actattaaat ctattagtta
     8161 aatatgataa ttctgattcc aatagcgctt caatagatag tttattaaaa atgaaagaaa
     8221 tccaatctaa gatggccctt cttcaaatgt taagttattt aaacaatcat caatggctat
     8281 tgaaaggaag agatcaaagg gatcaacgta aattgactat ttcgattaat aaaattcata
     8341 tagataaaat agaatatatg aataatgagc ttagtgacta tattgaacgt tattttggtc
     8401 aatatacatt tgattttagt tgtgactatg tatcgtattt attgtcgtca caagaattat
     8461 tgcggaatct aaagtgttat ttaaatatgt gtcaattatc attagaagag ttatacgttt
     8521 taggaatatt aaatctgcat aaaggtcaat tgacggttaa agaattacag ggtgaattcc
     8581 atcacccgat atttgcggtt agcccgattt taaagtgttt aattttgaag ggattagtta
     8641 aaaaagagag atgtgaatta gatgaaaggc gtgttattgt aacaatcaag cgagaaaagt
     8701 tttctaaagt cactatgctt attcaagcat gttataatta tcttgaaaag ggaatacaag
     8761 ttaatcttaa taataaatag ttaatcactt gtatttcata tacctatgaa gaatagttta
     8821 tagttttaag tgggagggat attatgattt tagataatgt gaatccaaat gatttatttc
     8881 caactgaaaa aaagggtccg tcagtgctag gaataattga gtatcaagtt caaggtgaga
     8941 atgaatttga aggggcattt atagcaacaa atgaaagatt aattatgaat gtagatatga
     9001 atggccaatt ttattataga agtattagtt ataatgaaat agagaagata gattatgatg
     9061 gtcaaactat aatgttcaaa tttaatattg gtaatgtccc aatgcatgat attaaaagtg
     9121 ctaacgttga aatgtttgtt gaatatgtaa aacaacatat gatagtataa tgtcaatgta
     9181 tgtaggttga taagaagagt ttgaatgata actcattgca tttaaagata aatggcatgc
     9241 gttatgattt aaagctcttc tttttttata ttttataaat tttgtgagca agccatccaa
     9301 ttaatataaa aaatatcaaa tcaaacacgt tgatatag
//
LOCUS       DALAGD010000104         9280 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.105, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000104 DALAGD010000000
VERSION     DALAGD010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9280
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.105"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(298..1050)
                     /locus_tag="O2701_002302"
     CDS             complement(298..1050)
                     /locus_tag="O2701_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-dependent peroxidase"
                     /protein_id="HCZ4294176.1"
                     /translation="MSQAAETLDGWYSLHLFYAVDWASLRIVPKDERDALVTEFQSFL
                     ENTATVRSSKSGDQAIYNITGQKADLLLWFLRPEMKSLNHIENEFNKLRIADFLIPTY
                     SYVSVIELSNYLAGKSDEDPYENPHIKARLYPELPHSDYICFYPMNKRRNETYNWYML
                     TMEERQKLMYDHGMIGRKYAGKIKQFITGSVGFDDFEWGVTLFSDDVLQFKKIVYEMR
                     FDETTARYGEFGSFFVGHIINTNEFDQFFAIS"
     gene            1218..2204
                     /gene="pta"
                     /locus_tag="O2701_002303"
     CDS             1218..2204
                     /gene="pta"
                     /locus_tag="O2701_002303"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCZ4294177.1"
                     /translation="MADLLNVLKDKLSGKNVKIVLPEGEDERVLTAATQLQATDYVTP
                     IVLGDETKVQSLAQKLDLDISNIELINPATSELKAELVQSFVERRKGKATEEQAQELL
                     NNVNYFGTMLVYAGKADGLVSGAAHSTGDTVRPALQIIKTKPGVSRTSGIFFMIKGDE
                     QYIFGDCAINPELDSQGLAEIAVESAKSALSFGMDPKVAMLSFSTKGSAKSDDVTKVQ
                     EAVKLAQQKAEEEKLEAIIDGEFQFDAAIVPGVAEKKAPGAKLQGDANVFVFPSLEAG
                     NIGYKIAQRLGGYDAVGPVLQGLNSPVNDLSRGCSIEDVYNLSIITAAQALQ"
     gene            2207..3043
                     /locus_tag="O2701_002304"
     CDS             2207..3043
                     /locus_tag="O2701_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCZ4294178.1"
                     /translation="MDLASKYFNGVNWRYIDHSSGLEPMQSFAFDDTFCESVGKDISD
                     NVVRTWIHQHTVILGIHDSRLPFLKDGIDYLTNEIGYNAIVRNSGGLGVVLDQGVLNI
                     SLMFKGQTETTIDEAFTVMYLLISKMFENENVDIDTMEIEHSYCPGKFDLSIDGKKFA
                     GISQRRVRGGIAVQIYLCVEGSGSERALMMQTFYEHALKGEVTKFKYPEIEPSCMASL
                     ETLLNKTITVQDVMFLLLYAIKDLGGVLNMTPITQEEWQRYDTYFDKMIERNKKMIDQ
                     MQ"
     gene            3630..4550
                     /gene="mvk"
                     /locus_tag="O2701_002305"
     CDS             3630..4550
                     /gene="mvk"
                     /locus_tag="O2701_002305"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HCZ4294179.1"
                     /translation="MTRKGYGESTGKIILIGEHAVTFGEPAIAVPFNAGKIKVLIEAL
                     ESGNYSSIKSDVYDGMLYDAPDHLKSLVNRFVELNNITEPLAVTIQTNLPPSRGLGSS
                     AAVAVAFVRASYDFLGKSLTKEELIEKANWAEQIAHGKPSGIDTQTIVSGKPVWFQKG
                     HAETLKTLSLDGYMVVIDTGVKGSTRQAVEDVHKLCEDPQYMSHVKHIGKLVLRASDV
                     IEHHNFEALADIFNECHADLKALTVSHDKIEQLMKIGKENGAIAGKLTGAGRGGSMLL
                     LAKDLPTAKNIVKAVEKAGAAHTWIENLGG"
     gene            4555..5538
                     /gene="mvaD"
                     /locus_tag="O2701_002306"
     CDS             4555..5538
                     /gene="mvaD"
                     /locus_tag="O2701_002306"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HCZ4294180.1"
                     /translation="MIKSGKARAHTNIALIKYWGKKDEALIIPMNNSISVTLEKFYTE
                     TKVTFNDQLTQDQFWLNGEKVSGKELEKISKYMDIVRNRAGIDWYAEIESDNFVPTAA
                     GLASSASAYAALAAACNQALDMQLSDKDLSRLARIGSGSASRSIYGGFAEWEKGYSDE
                     TSYAVPLESNHFEDDLAMIFVVINQHSKKVPSRYGMSLTRNTSRFYQYWLDHIDEDLA
                     EAKAAIQDKDFKRLGEVIEENGLRMHATNLGSTPPFTYLVQESYDVMALVHECREAGY
                     PCYFTMDAGPNVKILVEKKNKQQIIDKLLTQFDNNQIIDSDIIATGIEIIE"
     gene            5551..6627
                     /locus_tag="O2701_002307"
     CDS             5551..6627
                     /locus_tag="O2701_002307"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HCZ4294181.1"
                     /translation="MIQVKAPGKLYIAGEYAVTEPGYKSVLIALDRFVTATIEETDQY
                     KGTIHSKALHHNPVTFSRDEDSIVISDPHAAKQLNYVVTAIEIFEQYAKSCDIAMKHF
                     HLTIDSNLDDSNGHKYGLGSSAAVLVSVIKVLNEFYDMKLSNLYIYKLAVIANMKLQS
                     LSSCGDIAVSVYSGWLAYSTFDHEWVKHQIEDTTVEEVLIKNWPGLHIEPLQAPENME
                     VLIGWTGSPASSPHFVSEVKRLKSDPSFYGDFLEDSHRCVEKLIHAFKTNNIKGVQKM
                     VRQNRTIIQRMDKEATVDIETEKLKYLCDIAEKYHGASKTSGAGGGDCGITIINKDVD
                     KEKIYDEWTKHGIKPLKFNIYHGQ"
     gene            6804..7145
                     /locus_tag="O2701_002308"
     CDS             6804..7145
                     /locus_tag="O2701_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HCZ4294182.1"
                     /translation="MKNTFLICDECQAVNIRTLQKKLEKLDPDAEIVIGCQSYCGPGR
                     RKTFTFVNNRPLAALTEEELIEKVSQQLKKPRDPEEEERLRKRHEERKRRKVEQDRKL
                     KEKLEKRKAQQ"
     gene            complement(7197..8519)
                     /locus_tag="O2701_002309"
     CDS             complement(7197..8519)
                     /locus_tag="O2701_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HCZ4294183.1"
                     /translation="MKTYDLIVIGFGKAGKTLAKYAASTGQHVAVIEQSPKMYGGTCI
                     NIGCIPSKTLVHDGLEGKSFEASYNRKNDVVNALNNKNYHLLADDNNIDVLDFKAQFK
                     SNTEVNLLDQHDDIVDSITAPHIIINTGATSVIPNIKGLDQAKHVFDSTGLLNISYQP
                     KHLVIVGGGYIALEFASMFANLGSKVTVLERGESFMPREDQDVVAYGITDLENKGIAL
                     HTNVETTELSSDNHHTTVHTNVDNFEADAVLLAIGRKPNTDLALENTDIELGDRGEIK
                     VNAHLQTTVPHIYAAGDVKGGLQFTYISLDDYRIIKSALYGNQSRTTDNRGSVPYTVF
                     IDPPLSRVGLTSKEAAAQHYDYTEHQLLVSAIPRHKINNDPRGLFKVVINNENNMILG
                     ATLYGKQSEELINIIKLAIDQNIPYTVLRDNIYTHPTMAESFNDLFNF"
     gene            complement(8506..8946)
                     /locus_tag="O2701_002310"
     CDS             complement(8506..8946)
                     /locus_tag="O2701_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCZ4294184.1"
                     /translation="MNLEFNIAVHVLAFLTKHHSEKFNSSSLAELTCLNPVQLRRVTT
                     QLVDLKMIDTIRGKDGGYLANDQSADVSLATLYKHFVLEKEHHTRLFTGDEGSHCQIA
                     RNIATTMSHYQQDEQNIIINFYNGKTIKDVIEDIQKEDLCHENI"
     gene            9004..9105
                     /locus_tag="O2701_002311"
     CDS             9004..9105
                     /locus_tag="O2701_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294185.1"
                     /translation="MCQYLCLKSVELGVVIVMIYCLKVDALTVRLHN"
BASE COUNT         3219 a         1360 c         1778 g         2923 t
ORIGIN      
        1 acaaaaattg tatagagtag cgactgtata atttctattg aggttaacgt ttatatgtag
       61 tgatagtagt taaagttctc ccaaggaaga ctactcgggt acactttgct atgagcaaag
      121 tgtactttgt tattgataat acattagcac atatatataa gtttaaacat acagatttca
      181 actatttacc aaatcacgtc ttatgtatac gggaatatac tgaagataaa cgaaaaattc
      241 caagcttaaa ccgataagct tggaattttt ttattaattt ataaacgtac caatgtatta
      301 agaaatcgca aagaattgat cgaattcgtt tgtgttaata atatgtccta caaagaaact
      361 accgaattca ccgtatcgtg ctgttgtttc atcaaagcgc atttcgtata caattttttt
      421 gaattgtaat acgtcatctg agaacaatgt tacgccccat tcgaaatcat caaaccctac
      481 agaaccagta ataaattgtt tgattttgcc agcatatttt ctaccaatca taccatggtc
      541 atacattaat ttttggcgtt cttccatagt taacatgtac cagttataag tttcattacg
      601 acgtttgttc attggataga aacaaatata atcagaatgt ggtaattctg ggtataatct
      661 tgctttgata tgagggttct cataaggatc ttcatcagat ttaccagcta aataattgct
      721 caattcaatg actgatacat atgaatatgt agggattagg aagtcagcaa tgcgcaattt
      781 gttaaattca ttttcaatat gatttaaaga cttcatttca ggacgtaaga accataataa
      841 caaatctgct ttttgaccag ttatattata aatagcttga tcaccagatt ttgatgatct
      901 tacagttgct gtattttcta aaaatgattg aaattcagtg acaagtgcat cgcgttcgtc
      961 ctttggaact atacgtaatg atgcccaatc aactgcataa aataaatgta gactatacca
     1021 accatctaat gtttcggctg cttgactcat gtttatcgct ccttttcaaa aatcatcagt
     1081 tctttataaa ttttatcata aagcaaaggg gtaacgataa aattatgatt acaaattggt
     1141 gacgtggcat tatgaaataa aatggcgtat aattataccg tgaatgatta ataagattta
     1201 tattacagga ggacattatg gctgatttat taaatgtatt aaaagacaaa ctttctggta
     1261 aaaacgttaa aatcgtatta cctgaaggag aggacgaacg tgttctaaca gctgcaacac
     1321 aattacaagc aacagattat gttacaccaa tcgtgttagg tgatgagact aaggttcaat
     1381 ctttagcgca aaaacttgat cttgatattt ctaatattga attaattaat cctgcgacaa
     1441 gtgaattgaa agctgaatta gttcaatcat ttgttgaacg acgtaaaggt aaagcgactg
     1501 aagaacaagc acaagaatta ttaaacaatg tgaactactt cggtacaatg cttgtttatg
     1561 ctggtaaagc agatggttta gttagtggtg cagcacattc aacaggcgac actgtgcgtc
     1621 cagctttaca aatcatcaaa acgaaaccag gtgtatcaag aacatcaggt atcttcttta
     1681 tgattaaagg tgatgaacaa tacatctttg gtgattgtgc aatcaatcca gaacttgatt
     1741 cacaaggact tgcagaaatt gcagtagaaa gtgcaaaatc agcattaagc tttggcatgg
     1801 atccaaaagt tgcaatgtta agcttttcaa caaaagggtc tgctaaatca gacgacgtga
     1861 caaaagttca agaagctgtc aaattagcac aacaaaaagc tgaagaagaa aaattagaag
     1921 caatcattga tggcgaattc caatttgatg ctgcgattgt accaggtgtt gctgagaaaa
     1981 aagcgccagg tgctaaatta caaggtgatg caaatgtctt tgtattccca agtttagaag
     2041 ctggtaatat tggttacaaa attgcacaac gtttaggtgg atatgatgca gttggtccag
     2101 tattacaagg tttaaattct ccagtaaatg acttatcacg tggctgctca attgaagatg
     2161 tatacaatct ttcaattatt acagcagcgc aagccttaca ataacgatgg atttagcgag
     2221 taaatatttt aatggcgtca actggcgata tatcgatcat tcttctggat tagaacctat
     2281 gcaatctttc gcattcgatg atacattttg cgaaagtgtg ggcaaagata tatcagataa
     2341 tgttgtgcgt acttggattc atcaacatac tgttattctt ggtattcatg attcaagatt
     2401 gccgttttta aaagatggca ttgattattt aacgaatgag attggttata atgccattgt
     2461 tagaaattct ggtggcttag gtgtcgttct agatcaaggt gtattaaata tatcgctgat
     2521 gttcaaagga caaacagaaa caacgattga tgaagcgttt actgtgatgt acctcttaat
     2581 tagcaaaatg ttcgaaaatg agaatgttga tattgatacg atggaaattg aacattctta
     2641 ttgcccagga aaatttgact taagtatcga tggtaagaaa tttgcaggca tatcgcaacg
     2701 aagagttaga ggcggtattg ctgtacaaat ttatctttgt gttgaaggct ctggttcaga
     2761 acgtgcattg atgatgcaaa cattttatga acatgcttta aaaggtgaag tgactaaatt
     2821 taaatatcct gaaattgaac catcttgtat ggcctcatta gagacattgc ttaacaaaac
     2881 gattactgtt caagatgtaa tgtttttact attatatgca atcaaagatc ttggcggtgt
     2941 attaaatatg acgccaatta ctcaagaaga atggcaaaga tacgatacgt attttgataa
     3001 aatgattgaa agaaacaaga aaatgataga tcaaatgcaa tagttaaata tttaacagcc
     3061 cctttcaagt tatcgtttgg ggctgttatt tttgaaagta tccataaaaa gcataaatat
     3121 gttattgacc taatatttaa attgaacatc attaaattaa aagtaatgat atgatggaaa
     3181 taataaattt aataagaaaa tttcatgttt tacgtattaa atagattgag aatagggtat
     3241 atataatgcg tatgactatg aaagaaacga ccttctattg aaatgttcta atactttagt
     3301 agtaaatcta tatattccaa ttattatttg tagtattata gttagggtgg aaaattataa
     3361 attgtagggc tattttataa aaagataaag aatctgaaat atcactcgat aagatttaaa
     3421 ctttgaagta aacgaaagtt agttttatac taatcaagtt aagtcaaata taaattcaaa
     3481 ataaagatta tacataatat gaataatgtg agataaagac tgcaatgaaa tttaacgaga
     3541 atgcgtatgc aacttagaat atgatgtctt ttactaataa ataaaaataa tgaatcaagt
     3601 ataggataaa tataaaaggt gatattgaca tgacaagaaa aggatatggg gaatcgacag
     3661 gtaagattat tttaatagga gaacatgctg ttacatttgg agagcctgct attgcagtac
     3721 cgtttaacgc aggtaaaatc aaagttttaa tagaagcctt agagagcggg aactattcgt
     3781 ctattaaaag cgatgtttac gatggtatgt tatatgatgc gcctgaccat cttaagtctt
     3841 tggtgaaccg ttttgtagaa ttaaataata ttacagagcc gctagcagta acgatccaaa
     3901 cgaatttacc accatcacgt ggattaggat cgagtgcagc tgtcgcggtt gcttttgttc
     3961 gtgcaagtta tgatttttta gggaaatcat taacgaaaga agaactcatt gaaaaggcta
     4021 attgggcaga gcaaattgca catggtaaac caagtggtat tgatacgcaa acgattgtat
     4081 caggcaaacc agtttggttc caaaaaggtc atgctgaaac gttgaaaacg ttaagtttag
     4141 acggctatat ggttgttata gatactggtg tgaaaggttc aacaagacaa gcagtagaag
     4201 atgttcataa actttgtgag gaccctcagt acatgtcaca tgtaaaacat atcggtaagt
     4261 tagttttacg tgcgagtgat gtgattgaac atcataactt tgaagcctta gcggatattt
     4321 ttaatgaatg tcatgcggat ttaaaggcgt tgacagttag tcatgataaa atagaacaat
     4381 taatgaaaat tggtaaagaa aatggtgcga ttgctggaaa acttactggc gctggtcgtg
     4441 gtggaagtat gttattgctt gccaaagatt taccaacagc gaaaaatatt gtaaaagctg
     4501 tagaaaaagc tggtgcagca catacttgga ttgagaattt aggaggttaa tgcgttgatt
     4561 aaaagtggca aagcacgtgc acatacgaat attgcactta taaaatattg gggtaaaaaa
     4621 gatgaagcac taatcattcc aatgaataat agcatatctg ttacattaga aaaattttac
     4681 actgaaacga aagtcacttt taacgaccag ttaacacagg atcaattttg gttgaatggt
     4741 gaaaaggtta gtggcaaaga attagagaaa atttcaaaat atatggatat tgtcagaaat
     4801 agagctggca tcgattggta tgcagaaatt gaaagcgaca attttgtacc aacagcagca
     4861 gggttggctt catcggcaag cgcatatgca gctttagcag cagcttgtaa tcaagcgcta
     4921 gacatgcagc tgtcagataa ggatttatcg agattggcgc gaattggttc gggttctgcg
     4981 tcgcgtagta tttatggtgg atttgcagaa tgggaaaaag ggtatagtga tgagacgtca
     5041 tatgccgttc cacttgaatc gaatcatttt gaagatgacc ttgccatgat atttgttgtg
     5101 attaatcaac attctaaaaa ggtacctagt cgatatggta tgtcattgac acgaaacaca
     5161 tcaaggtttt atcaatattg gttagatcat attgatgaag atttagctga agcaaaagca
     5221 gcgattcaag acaaagattt taaacgcctt ggtgaagtaa ttgaagaaaa tggtttgcgt
     5281 atgcatgcca cgaatctagg atcaacaccg ccgttcacat atcttgtgca agaaagttat
     5341 gatgtcatgg cgcttgttca cgaatgccga gaagcggggt atccgtgtta ttttacaatg
     5401 gatgcgggac ctaatgtgaa aatacttgta gaaaagaaaa acaagcaaca gattatagat
     5461 aaattattaa cacagtttga taataaccaa attattgata gtgacattat tgccacagga
     5521 attgaaataa ttgagtaagg aagagataaa atgattcagg tcaaagcacc cggaaaactt
     5581 tatattgctg gagaatatgc tgtaacagaa ccaggatata aatctgtact tattgcgtta
     5641 gatcgttttg taactgctac tattgaagaa acagaccaat ataaaggtac cattcattca
     5701 aaagcattac atcataaccc agttacattt agtagagatg aagatagtat tgtcatttca
     5761 gatccacatg cagcaaaaca attaaattat gtggtcacag ctattgaaat atttgaacaa
     5821 tacgcgaaaa gttgcgatat agcgatgaag cattttcatc tgactattga tagtaattta
     5881 gatgattcaa atggtcataa atatggatta ggttcaagtg cagcagtact tgtgtcagtt
     5941 ataaaagtat taaatgaatt ttatgatatg aagttatcta atttatacat ttataaacta
     6001 gcagtgattg caaatatgaa gttacaaagt ttaagttcat gcggagatat tgctgtgagt
     6061 gtatatagtg gatggctagc gtatagtact tttgatcatg aatgggttaa gcatcaaatt
     6121 gaagatacta cggttgaaga agttttaatc aaaaactggc ctggattgca catcgaacca
     6181 ttacaagcac ctgaaaatat ggaagtactt atcggttgga ctggctcacc ggcgtcatca
     6241 ccacactttg ttagcgaagt gaaacgtttg aaatcagatc cttcatttta cggtgacttc
     6301 ttagaagatt cacatcgttg tgttgaaaaa cttattcatg cttttaaaac aaataacatt
     6361 aaaggtgtgc aaaagatggt gcgtcagaat cgtacaatta ttcaacgtat ggataaagaa
     6421 gctacagttg atatagaaac tgaaaagcta aaatatttgt gtgatattgc tgaaaagtat
     6481 cacggcgcat ctaaaacatc aggcgctggt ggtggagact gtggtattac aattatcaat
     6541 aaagatgtag ataaagaaaa aatttatgat gaatggacaa aacatggtat taaaccatta
     6601 aaatttaata tttatcatgg gcaataaatg atttgatagg ggagcaatca tctaatttaa
     6661 ttagaagata aatgctccat tttttattgt cagccgtgtt acgtatcttg aaaaaaatat
     6721 taaaaagagt aaaatagata gagttaacga aaaaattaat gaaatcgacg atatagatat
     6781 gataaagaaa ggtgggtagc aatatgaaaa atacattcct tatttgtgat gaatgtcagg
     6841 cagtcaatat aagaacgtta caaaagaagt tggaaaaatt agatcccgat gctgaaatcg
     6901 tgataggttg tcaatcttat tgtggacctg gacgccgaaa aacattcact tttgttaata
     6961 accgcccact ggctgcgctt actgaagaag aattaatcga aaaagtttct caacaattaa
     7021 agaaaccacg tgatcctgaa gaagaagagc gtttaagaaa acgacatgaa gaacgtaaac
     7081 gtcgtaaagt agaacaagat agaaagctta aagaaaaatt agaaaagcga aaagcacaac
     7141 aataaagcct gatggcagca tcattcaatg cgtgccacca ggtttttatg ttttgtctag
     7201 aaattaaata aatcattaaa tgattcggcc atcgtaggat gcgtataaat attatctcgt
     7261 aatacggtat atggaatgtt ttgatcaatc gcaagtttaa ttatattaat taattcttca
     7321 gattgcttac catataatgt agcacctaaa atcatattat tttcattatt aatgactact
     7381 ttaaataaac ctcttggatc attgttaatt ttatgacgag gtatagcact tactaaaagt
     7441 tgatgttcag tgtaatcata atgttgagcg gcagcttctt tactagttaa tccaacacgt
     7501 gataatggtg gatctataaa tactgtataa ggcacgctgc ctctattgtc agtcgtacgt
     7561 gactgattac catataacgc tgatttgata attcgataat catctaaaga tatatacgta
     7621 aattgaagtc cgcctttaac atcacctgca gcataaatat gcggcacagt tgtttgaaga
     7681 tgagcattga ctttaatttc gcctctgtcg cctaattcga tatcagtatt ttctaaagct
     7741 aaatccgtat tcggtttgcg cccgatagcc aaaagtactg catcagcctc aaagttatca
     7801 acgttggtat ggactgttgt atgatgattg tcagatgaca attcagtcgt ttcaacattt
     7861 gtatgcaatg caatgccttt attttctaag tcagtaatac catatgcaac gacatcttga
     7921 tcttcgcgtg gcataaatga ttcgccacgt tctaatactg ttaccttact acctaaattc
     7981 gcaaacattg aagcaaattc taaggcgata taaccgccac ctacaataac gaggtgctta
     8041 ggttgatagc taatgtttaa taaacctgtc gaatcgaaga cgtgtttagc ttgatcaagg
     8101 cctttaatgt taggaatgac agaggtagca ccggtattaa taatgatatg aggtgcagta
     8161 atactatcga cgatatcgtc atgttgatct aataaattca cttcagtatt agatttaaac
     8221 tgcgctttaa aatccagtac atcaatgttg ttatcgtctg ctaataagtg gtaattttta
     8281 ttgtttagcg cattgacaac atcgttttta cggttataac ttgcttcaaa agatttgcct
     8341 tctaatccat catgtacaag tgtcttcgaa ggtatacatc ctatgtttat acaagtgcct
     8401 ccatacattt tcggagattg ttcgataact gcgacgtgtt gacctgttga tgcagcgtat
     8461 ttcgctaaag ttttaccagc tttcccaaat cctattacaa ttaaatcata tgttttcatg
     8521 acataaatcc tccttttgaa tgtcttcaat gacatctttg attgtttttc cattataaaa
     8581 attaataatg atattctgtt cgtcttgctg ataatgtgac atggtagttg caatattacg
     8641 agcaatttga cagtgactgc cttcgtcgcc agtaaataga cgtgtgtggt gttctttctc
     8701 taagacaaaa tgtttatata atgttgctag agagacatca gcactttgat catttgctaa
     8761 ataaccgcca tctttacctc gtattgtgtc aatcattttt aaatcgacaa gttgagtcgt
     8821 cacgcgtcgt aattgaacag gatttaaaca agttaattct gctaatgaac tactattgaa
     8881 tttttctgaa tgatgcttag ttaaaaaagc taatacatgc acggcaatgt taaattctaa
     8941 attcaatgtt tctcacctca taattgtaac tgtattagtt acaattaaaa atcattttgc
     9001 tcaatgtgtc aatacttatg tttgaaaagt gttgaattag gtgtggttat tgtgatgatt
     9061 tattgtttga aagttgatgc attaacggtg agattgcaca attgaaattt taattatgta
     9121 tgaactgata gatatgaaat gaatattgaa aatgagagca ataaaatttt gaatgatgat
     9181 gttgtgtgat tgtataaact tattcgtatt ttagatacgt tttgatattt cgcatgatga
     9241 ggaaatactt ttaacaactg atgacatatt ttaatagtga
//
LOCUS       DALAGD010000105         9262 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.106, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000105 DALAGD010000000
VERSION     DALAGD010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9262
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.106"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(168..1070)
                     /gene="cvfB"
                     /locus_tag="O2701_002312"
     CDS             complement(168..1070)
                     /gene="cvfB"
                     /locus_tag="O2701_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HCZ4294186.1"
                     /translation="MALDKDIVGSIEFLEVVGLQGSTYLLKGPNGENVKLNQSEMNDD
                     DELEVGEEYSFFIYPNRSGELFATQNMPDITKDKYDFAKVLKTDRDGARIDVGLPREV
                     LVPWEDLPKVKSLWPQPGDYLLVTLRIDRENHMYGRLASESVVENMFTPVHDDNLKNE
                     VIEAKPYRVLRIGSFLLSESGYKIFVHESERKAEPRLGESVQVRIIGHNDKGELNGSF
                     LPLAHERLDDDGQVIFDLLVEYDGELPFWDKSSPEAIKEVFNMSKGSFKRAIGHLYKQ
                     KIINIETGKIALTKKGWSRMDSKE"
     gene            1217..2818
                     /locus_tag="O2701_002313"
     CDS             1217..2818
                     /locus_tag="O2701_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCZ4294187.1"
                     /translation="MLQVTDVSLRFGDRKLFEDVNIKFTEGNCYGLIGANGAGKSTFL
                     KILSGELDSQTGHVSLGKNERLAVLKQDHYAYEDERVLDVVIKGHERLYEVMKEKDEI
                     YMKPDFSDEDGIRAAELEGEFAEMNGWNAEADAANLLSGLGIDPTLHDKKMAELENNQ
                     KIKVLLAQSLFGEPDVLLLDEPTNGLDIPAISWLEDFLINFDNTVIVVSHDRHFLNNV
                     CTHIADLDFGKIKVYVGNYDFWYQSSQLAQKMAQEQNKKKEEKMKELQDFIARFSANA
                     SKSKQATSRKKQLEKIELDDIQPSSRRYPFVKFTPEREIGNDLLIVQNLSKTIDGEKV
                     LDNISFTMNPNDKAILIGDSEIAKTTLLKILAGEMEPDEGSYKWGVTTSLSYFPKDNS
                     EFFEGVNMNLVDWLRQYAPEDEQTETFLRGFLGRMLFSGEEVKKKASVLSGGEKVRCM
                     LSKMMLSSANVLLLDEPTNHLDLESITAVNDGLKSFKGSIIFTSYDFEFINTIANRVI
                     DLNKQGGVSKEIPYEEYLQEIGVLK"
     gene            3678..4883
                     /locus_tag="O2701_002314"
     CDS             3678..4883
                     /locus_tag="O2701_002314"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCZ4294188.1"
                     /translation="MVTRSVLKFGGSSVSDFTKIKRIAEMLKERVNQDEQLIVVVSAM
                     GNTTDQLMTNVSTLTKAPKQQELALLLTTGEQQTVSYLSMVLNDIGMNAKAMTGYQAG
                     IKTIGHHLKSKIAQINPQTFEQAFQENDILVVAGFQGINEHQELTTLGRGGSDTTAVA
                     LAVSNQIPCEIYTDVDGVYATDPRLLPKAKRLDIVSYEEMMEMSALGAGVLETRSVEL
                     AKNYNIPLYLGKTLSNVKGTWIISNEEILEKKAVTGVALDKHMMHVTISYPLPDNQLL
                     TQLFTELEEGAVNVDMISQIVNLDGLQLSFTIKDSDFHQISMILETLKNQYEALAYKI
                     NEHYVKISLIGSGMRDMSGVASKAFLTLIENNIPFYQTTTSEISISYVIDDFNGQQAV
                     EKLYDAFNI"
     gene            4947..5936
                     /locus_tag="O2701_002315"
     CDS             4947..5936
                     /locus_tag="O2701_002315"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCZ4294189.1"
                     /translation="MTKLAVVGATGLVGTKMLETLNRKNIPFDELVLFSSARSAGQEV
                     EFQGKTYTVQELTDARASEHFDYVLMSAGGGTSEHFAPLFEKAGAIVIDNSSQWRMAE
                     DIDLIVPEVNEPTFTRGIIANPNCSTIQSVVPLKVLQDAYGLKRVAYTTYQAVSGSGM
                     KGKKDLAEGVNGKAPEAYPHPIYNNVLPHIDVFLENGYTKEEQKMIDETRKILNAPDL
                     KVTATCARVPVQDSHSVEIDVTLDKETTAEDIKALFDQDDRVVLVDNPENNEYPMAIN
                     STNKDEVFVGRIRRDDSLENTFHVWCTSDNLLKGAALNAVQVLEQVMRLKGAN"
     gene            5938..6825
                     /locus_tag="O2701_002316"
     CDS             5938..6825
                     /locus_tag="O2701_002316"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCZ4294190.1"
                     /translation="MTHLFEGVGVALTTPFTNNKVNIEALKTHVNFLLENNAQAIIVN
                     GTTAESPTLTTDEKERILKTVIDLVDKRVPVIAGTGTNDTEKSIQASIQAKALGADAI
                     MLITPYYNKTNQRGLVKHFEAIADAVKLPVVLYNVPSRTNMTIEPETVEILSQHPYIV
                     ALKDATNDFEYLEEVKKRIDTNSFALYSGNDDNVVEYYQRGGQGVISVIANVIPKEFQ
                     ALYDAQQSGLDIQDQFKPIGTLLSALSVDINPIPIKALTSYLGFGNYELRLPLVSLED
                     TDTKVLRETYDTFKAGENE"
     gene            6822..7544
                     /locus_tag="O2701_002317"
     CDS             6822..7544
                     /locus_tag="O2701_002317"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCZ4294191.1"
                     /translation="MKILLIGYGAMNQRVARLAEEKGHEIVGVIENTPKATTPYQQYQ
                     HIADVKGADVAIDFSNPNLLFPLLDEDFHLPLVVATTGEKEKLLNKLDELSQNMPVFF
                     SANMSYGVHALTKILAAAVPLLDDFDIELTEAHHNKKVDAPSGTLEKLYDVIVSLKEN
                     VTPVYDRHELNEKRQPQDIGIHSIRGGTIVGEHEVLFAGTDETIQITHRAQSKDIFAN
                     GAIQAAERLVNKPNGFYTFDNL"
     gene            7571..8290
                     /gene="dapD"
                     /locus_tag="O2701_002318"
     CDS             7571..8290
                     /gene="dapD"
                     /locus_tag="O2701_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HCZ4294192.1"
                     /translation="MVQHLTAEEIIQYISDAKKSTPIKVYLNGNFEGITYPESFKVFG
                     SEQSKVIFCEADDWKPFYEAYGSQFEDIEIEMDRRNSAIPLKDLTNTNARIEPGAFIR
                     EQAIIEDGAVVMMGATINIGAVVGEGTMIDMNATLGGRATTGKNVHVGAGAVLAGVIE
                     PPSASPVIIEDDVLIGANAVILEGVRVGKGAIVAAGAIVTQDVPAGAVVAGTPAKVIK
                     QASEVQDTKKEIVAALRKLND"
     gene            8433..>9262
                     /locus_tag="O2701_002319"
     CDS             8433..>9262
                     /locus_tag="O2701_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCZ4294193.1"
                     /translation="MNELEFVTKHRRHLHQHPELSLHEFETTAYIKAFLDSLNIKYDC
                     PLETGVIAYLEGNGSHTIAYRADIDALPILEENDVPYRSQSDHVMHACGHDGHTTALM
                     LFVQRCKDMQDAGQLPQNVVFIFQPAEETGGGANRLIKAGAFDKYPIEAVFGIHVNPF
                     ADEGIAVIRDEEITASATEYRFFLTGLSSHVADKEQGHSCGEALQHVLTQISQIQQFH
                     LNGLKRNIVHIGHFKAGEAINTVPSNGYLEGTIRTYDIDDLTIVKNQMHKIAESVKLL
                     "
BASE COUNT         3188 a         1483 c         1712 g         2879 t
ORIGIN      
        1 ttaatagaaa tcagcttttt tacattgcct aagaacttaa tgtcccagcc tattcgtttg
       61 aattatttaa ttttttactt tgaaaggaat gtttaaaaaa ctacttcatc tgtccgattt
      121 aatattgaat aaaaaaaaca cgcatcctac gacacgtgta aaaatgatta ttcttttgag
      181 tccattcgac tccaaccttt tttagttaaa gcgattttac ctgtttctat attaataatc
      241 ttctgtttat ataagtgacc gattgcacgt ttgaatgaac ctttactcat attgaatact
      301 tctttaatcg cttcagggct tgatttgtcc cagaatggta attcaccatc atattcaact
      361 agtaaatcaa agatgacttg gccgtcatcg tctaaacgtt catgtgcaag tggtaaaaat
      421 gaaccattta actcaccttt atcattatgc ccgataattc taacttgaac agattcacct
      481 aatcttggtt cagctttacg ttctgattca tgtacgaaaa ttttgtaacc tgattcgctt
      541 aataaaaagc taccaattcg taatacgcgg taaggtttgg cttcaatgac ttcgtttttt
      601 aaattatcgt cgtgtacagg tgtaaacata ttttctacaa cagattcact cgctaaacgt
      661 ccatacatat gattctcacg gtcaattcgt aatgtaacta gcaaataatc accaggttgt
      721 ggccatagtg atttcacttt tggtaaatct tcccatggta ctaacacttc acggggtaat
      781 ccaacatcta tacgtgcccc atcgcgatcc gttttaagta ctttagcaaa gtcatattta
      841 tctttcgtaa tatcaggcat attttgagtt gcaaataatt cacctgaacg gtttggataa
      901 atgaagaaac tatattcttc acctacttct aattcatcat catcgttcat ttctgattgg
      961 tttaacttta cgttttcacc gtttggtcct tttaaaaggt aagttgaacc ttgtaaccct
     1021 actacttcaa ggaattctat agaacctact atatctttgt ctaatgccat gttattctcc
     1081 ttcgattctt gtattcttta ttattataac atgtgtgagt cattctatca ttacaactta
     1141 tatgaggtcg aaactgtgac gataatatga tataattctt ccgttagaat atcgaatgaa
     1201 aaaaaggaga aaatgcatgt tacaagtaac tgatgtgagt ttacgttttg gagatcgtaa
     1261 actatttgaa gatgtaaata ttaaatttac agaaggtaat tgttatggat taattggtgc
     1321 gaatggtgca ggtaaatcaa cattcttaaa aatattatct ggtgaattag attctcaaac
     1381 aggacatgtt tcattaggga aaaatgaacg tctagctgtt ttaaaacagg accactatgc
     1441 ttatgaagat gaacgcgtgc ttgatgttgt aattaaaggt cacgaacgtc tttatgaggt
     1501 tatgaaagaa aaagatgaaa tctatatgaa gccagatttc agtgatgaag atggtatccg
     1561 tgctgctgaa cttgaaggtg aatttgcaga aatgaatggt tggaatgctg aagctgatgc
     1621 tgctaacctt ttatctggtt taggtatcga tccaacttta cacgataaaa aaatggctga
     1681 attagaaaac aaccaaaaaa ttaaagtatt attagcgcaa agtttattcg gtgaaccaga
     1741 cgtactatta ctggatgagc ctactaacgg tctcgatatt ccagcaatca gttggttaga
     1801 agatttctta attaactttg ataatactgt tatcgtagta tcgcatgacc gtcatttctt
     1861 aaataatgta tgtactcata tcgctgattt agacttcggt aaaattaaag tttatgttgg
     1921 taactatgat ttttggtatc aatctagtca gttagctcaa aagatggctc aagaacaaaa
     1981 caagaaaaaa gaagaaaaaa tgaaagagtt acaggacttt attgctcgtt tctcagctaa
     2041 cgcttctaaa tctaaacaag caacaagtcg taaaaaacaa ctcgagaaaa ttgaattaga
     2101 tgatattcaa ccatcatcaa gaagatatcc tttcgttaaa ttcacacctg agcgcgaaat
     2161 cggtaatgac ttactaatcg ttcaaaatct atctaaaacg attgacggtg aaaaagtatt
     2221 agataatatt tcattcacaa tgaatccaaa tgataaagca attttaattg gggatagtga
     2281 aattgcgaaa accacattgc ttaaaatatt agccggtgaa atggaaccag acgaaggttc
     2341 atataaatgg ggtgtaacaa cgtcattaag ttacttccct aaagataact cagaattctt
     2401 tgagggcgtt aatatgaacc ttgtagattg gttaagacaa tacgcaccag aagatgagca
     2461 aactgaaaca tttttacgcg gcttcttagg ccgtatgcta tttagtggag aagaagttaa
     2521 gaaaaaagct agtgtacttt caggtggaga aaaagtacgt tgtatgctaa gtaaaatgat
     2581 gttatcaagt gcaaacgttc ttttacttga tgaaccaaca aaccacttag acttagaaag
     2641 tattactgct gttaatgatg gacttaaatc attcaaaggt tctattatct ttacttcata
     2701 tgactttgaa tttatcaata caatcgcaaa ccgtgttatc gatttaaata aacaaggtgg
     2761 cgtttcaaaa gaaattccat atgaagaata tttacaagaa atcggcgttt taaaataaac
     2821 ataatatgta gaatttcttt tcgaaaatct ctgtattgag gcccgaccaa cttgcacatt
     2881 attgtaagtt ggcttactgt caccttctat attgggactt attccgcaac ttgtattgtt
     2941 tgtagaaatt aggaatccaa tttctctatg tgttgcaccg acaaggattg aaaaaagcta
     3001 gctacaaagt cattttcatt cagtcaacta ctgtcaatat aacattgcag ggcctagggc
     3061 attgattcat gtccgagact atattcaatt taatagacat taactgggcg tctcgatgct
     3121 aatatttata gcatcgaggt gtccttttta ttttattttt taacacatca gcatagtgaa
     3181 caccccttct ctaaattaca aatacagtta tcaacgcatt aaaagagtac atttatttaa
     3241 tgtgcatatt gattatgcta taagtatttt gaattttatt tttcaaaaag tctcattatc
     3301 accacctttt aaaatataag caacgctata acttgaattt tcaggttatt ccaaaaaatc
     3361 taaattatcg gtttacattc atatttgtca tatgtataat gaacacatac caatttaata
     3421 atattttgat gaggcgcatc aatcatgagt aaagtttaga ttactgtctg ctaacagcta
     3481 aatttgaaag ggtgcgatgc cgaagcaatt ataatagcag ttataatttg ttggactttt
     3541 tggttaagag ctgagagttt gtcattattt aaaaataatg gagtgcatca cttgtatata
     3601 gattaagagc aagttcgcat tccgaacttg ttcttttttt attattgtgt gcccttccta
     3661 acaattagga ggattatatg gtaacaagaa gtgtgttgaa atttggcgga tcatccgtca
     3721 gtgattttac aaaaataaaa aggatcgctg aaatgttaaa ggagcgagtc aatcaagatg
     3781 aacaattaat tgtcgttgta agtgctatgg gtaacacaac agatcaatta atgacgaatg
     3841 tatcaacctt gactaaagca ccaaaacaac aagaactggc attattattg acaaccggag
     3901 agcaacaaac tgtatcttat ttatcaatgg tattaaatga tatcggtatg aatgccaaag
     3961 caatgactgg ctatcaagcg ggtattaaaa ccattggcca tcatttaaaa agtaaaattg
     4021 ctcaaattaa tcctcaaaca tttgaacaag cctttcaaga aaacgatatt ttagtagttg
     4081 ctggatttca aggcatcaat gaacatcagg aattaacaac tttaggcaga ggtggttctg
     4141 atacgaccgc tgtggcactt gctgttagta atcaaatacc ttgtgaaatt tataccgacg
     4201 ttgatggtgt gtatgccact gacccaagac ttttaccaaa ggctaaacga ctagacatcg
     4261 tctcatatga agaaatgatg gaaatgagcg ctttaggtgc tggtgtactt gaaacaagaa
     4321 gtgttgaatt agctaaaaac tataatatcc ctttatattt aggaaaaact ttatcgaacg
     4381 tgaaaggaac atggattata tcaaatgaag aaatattaga gaaaaaagca gttactggtg
     4441 tggctttgga taaacatatg atgcatgtaa caattagtta tcccctacct gacaatcagc
     4501 tacttaccca actatttacg gaacttgaag aaggtgctgt aaatgttgat atgatttcac
     4561 aaatcgtcaa cttggatggg ctacaactat ccttcacgat taaagatagt gattttcatc
     4621 aaatttctat gattcttgaa acattaaaga atcaatatga agcattagct tataaaatca
     4681 atgagcatta tgtcaaaatt tcattaattg gctcaggcat gcgtgatatg tcaggtgtgg
     4741 catcaaaagc atttttgaca ttaattgaaa ataatatacc tttctaccaa acaacaacat
     4801 ctgaaataag tatttcatac gtcattgatg attttaatgg gcaacaagcg gtagaaaaac
     4861 tatatgacgc atttaacatt taatggtaaa atgattgtta aaatattcta aaaattggaa
     4921 attattataa aatggagtga caagttatga caaagttagc agttgtgggt gcaacaggat
     4981 tagtaggtac aaaaatgttg gagacattaa atcgtaaaaa tattcctttc gatgaattag
     5041 tattattttc atcagcacgt tctgcagggc aagaagttga atttcaagga aaaacatata
     5101 cagttcaaga attaactgat gctcgtgcaa gtgaacattt cgattatgta ttaatgagtg
     5161 ctggtggcgg tacaagcgaa cactttgccc cactttttga aaaagctggt gcaatcgtta
     5221 tagacaattc aagtcaatgg cgtatggcag aagatattga tttaatcgtt ccggaagtca
     5281 atgaacctac atttacaaga ggtatcattg ccaatccaaa ctgctctacg attcaatctg
     5341 ttgtacctct aaaagtattg caagatgctt atggtttaaa acgagtggca tatacaacat
     5401 atcaagctgt atcaggttca gggatgaaag gtaagaaaga tttagctgaa ggtgtaaatg
     5461 gtaaagcacc agaagcatat ccacatccaa tttataataa tgtgttaccg catattgatg
     5521 tgtttttaga aaacggatat acaaaagaag aacaaaaaat gattgatgag acgagaaaaa
     5581 ttttaaatgc gccagactta aaagtaacag caacatgcgc acgtgtgcct gttcaagata
     5641 gtcatagtgt tgaaattgat gtaacgcttg acaaagaaac aacagcagaa gatattaaag
     5701 cgttatttga tcaagatgac cgcgttgttt tagtagacaa tccagagaac aatgaatatc
     5761 caatggcaat caattctact aataaagatg aagtgtttgt tggccgtata cgtagagatg
     5821 attcattaga aaatactttc catgtatggt gtacatcaga caatttatta aaaggtgctg
     5881 cattaaatgc tgtacaagta ttggaacaag ttatgcgttt aaaaggagcg aattaaaatg
     5941 acacatttat ttgagggtgt tggcgttgca cttacaaccc cttttacaaa taacaaagtt
     6001 aatattgaag ctttgaaaac acacgttaat tttttactag aaaataatgc ccaagcaatc
     6061 atcgttaatg gaactactgc tgagagccct actttaacaa cagatgaaaa agaacgcatt
     6121 ctaaaaacag ttattgatct tgtagataaa cgtgttcctg tcatagcagg aactggcact
     6181 aatgatactg aaaagtcaat ccaagcttca atccaagcta aagccttagg ggctgatgca
     6241 attatgttaa ttacgcccta ctacaacaaa acgaaccaac gtggtttagt caaacacttt
     6301 gaagcgattg cagatgctgt gaaattacca gtcgtgctgt acaatgttcc ttcaagaacg
     6361 aacatgacaa ttgaaccaga aactgtagaa atattaagtc aacatcctta tatagttgct
     6421 ttaaaagatg ctacgaatga ttttgagtat ttagaagaag tgaaaaagcg cattgataca
     6481 aattcatttg cattatatag tggcaatgat gacaacgtcg tcgaatacta tcaacgtggc
     6541 ggtcaagggg ttatctctgt tattgccaat gtcattccta aagaatttca agcgttatac
     6601 gatgctcaac aaagtggatt agatattcaa gatcaattta aaccaatcgg cacactgtta
     6661 tcagctttat cagttgatat taacccaatt cctattaaag ctctaacaag ttatttagga
     6721 tttggaaatt atgaattacg tctaccattg gttagcctag aagatacaga tactaaagtg
     6781 cttcgtgaaa catatgacac atttaaagcg ggtgaaaatg agtgaaaata ttactaattg
     6841 gctatggcgc aatgaatcaa cgcgttgcta gattagcaga agaaaaagga catgaaatcg
     6901 ttggggtcat tgaaaataca ccgaaagcaa caacgccata tcaacaatat caacatattg
     6961 cagatgttaa aggtgccgat gttgcaatag atttttcaaa tccaaatctg cttttccctt
     7021 tattagatga agattttcat ttgccattag ttgtggcaac aactggcgag aaagaaaaac
     7081 tacttaataa gttagatgaa ttgagtcaaa atatgcctgt gtttttcagc gcgaacatga
     7141 gttatggcgt tcatgcattg actaaaattt tagcagctgc tgttccccta cttgatgatt
     7201 tcgacatcga attgactgag gcacatcata ataaaaaagt agatgcacca agtggtacgt
     7261 tagaaaaatt gtatgatgtg atcgtatctt tgaaagaaaa tgtaacacct gtgtatgata
     7321 gacatgaatt aaatgaaaaa cgccagccac aagatattgg tatacattct attcgtggag
     7381 gtacgattgt cggtgaacat gaagttctat ttgctggcac tgatgaaacg attcaaatca
     7441 cgcatcgtgc acaatcaaaa gatatttttg cgaatggtgc aatacaagca gcagaacgct
     7501 tagttaataa accaaacggc ttttatacgt ttgacaactt ataaacatat taaaggagat
     7561 cgattatttt atggtacaac atttaacagc tgaagaaatt attcaatata taagtgatgc
     7621 taaaaagtct acaccaataa aagtatattt aaatggtaat tttgaaggca tcacatatcc
     7681 agaaagtttt aaagtatttg gttcagaaca atctaaagta atcttttgtg aagcggatga
     7741 ttggaaacct ttttacgaag catatggtag tcaattcgaa gatatagaaa ttgaaatgga
     7801 tcgtcgcaat tctgctattc cattaaaaga cttaacaaat acgaatgcac gaattgaacc
     7861 aggtgcgttt attagagaac aagccattat tgaagatggt gctgtcgtta tgatgggcgc
     7921 aacaattaat attggcgcag tcgttggcga aggtacaatg attgatatga atgctactct
     7981 cggtggtcgt gctacaactg gtaaaaatgt acatgtaggg gctggcgcag tattagcagg
     8041 tgtgattgaa ccccctagtg cttcaccggt tataatcgag gatgatgtat taatcggtgc
     8101 aaatgcagtt attttagaag gtgtacgtgt tggtaaaggt gctattgttg cagctggcgc
     8161 gattgtgaca caagatgtac cagctggtgc agttgttgct ggtacacctg caaaagtgat
     8221 taagcaagct tctgaagtac aagatactaa aaaagagatt gtagcagcat taagaaaact
     8281 gaatgactag tgacgtcaac gtataataat ttcaaggttg agatacgatt atgtctcaac
     8341 cttattgttt agcattgaat aaacttatga tcacaaattt cgaaatacgt tacactattt
     8401 atatagtaat ttaacacaaa ggattgataa ttatgaatga attagaattt gttacgaaac
     8461 atcgccgtca tttacatcaa catcctgaat taagcttaca tgaatttgaa acaactgctt
     8521 atattaaagc gtttttagat agtttaaata ttaaatacga ttgcccattg gaaactggcg
     8581 tcattgcata cttagaaggt aatggctcac atacgatagc gtatagagct gatattgatg
     8641 cgttacctat tttagaggaa aatgatgtgc cttatcgcag tcaatctgat catgtgatgc
     8701 atgcttgtgg acatgatggt catacaactg cattaatgct ttttgtacaa cgttgcaaag
     8761 acatgcaaga tgcaggtcaa ttaccgcaaa atgtcgtttt cattttccaa cctgcagaag
     8821 aaactggtgg cggtgcaaat cgattaataa aagccggtgc ctttgataag tatccaattg
     8881 aagcggtatt tggtattcat gttaacccat ttgctgatga aggcattgca gtgataagag
     8941 atgaagaaat tacggccagc gcaacagagt atcgcttttt cttaacaggc ctgtcaagtc
     9001 atgttgctga taaagaacaa ggtcattctt gtggtgaagc attacaacat gtattaactc
     9061 aaatatcaca aattcaacaa tttcacctta acggtttgaa acgaaatatt gttcatattg
     9121 gtcattttaa agctggtgaa gcgattaaca ctgtaccaag taatggctat ttagaaggta
     9181 ctattcgtac atatgatatt gatgatttaa caatcgttaa aaatcaaatg cacaagatag
     9241 cagaaagtgt caagcttctg tt
//
LOCUS       DALAGD010000106         8975 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.107, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000106 DALAGD010000000
VERSION     DALAGD010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8975
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.107"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(<1..546)
                     /locus_tag="O2701_002320"
     CDS             complement(<1..546)
                     /locus_tag="O2701_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001628197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCZ4294194.1"
                     /translation="MINNHELLGIHHVTAMTDDAERNYKFFTEVLGMRLVKKTVNQDD
                     IYTYHTFFADDVGSAGTDMTFFDFPNITKGQAGTNSITRPSFRVPNDDALTYYEQRFD
                     EFGVKHEGIQELFGKKVLPFEEVDGQVYQLISDELNEGVAPGVPWKNGPVPVDKAIYG
                     LGPIEIKVSYFDDFKNILETVY"
     gene            complement(624..1058)
                     /locus_tag="O2701_002321"
     CDS             complement(624..1058)
                     /locus_tag="O2701_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCZ4294195.1"
                     /translation="MDRTKQSLNVFVGMNRALDTLEQITKEDVKRYGLNITEFAVLEL
                     LYNKGPQPIQRIRDRVLIASSSISYVVSQLEDKGWITREKDKDDKRVYMACLTEKGQS
                     QMADIFPKHAETLTKAFDVLTKDELTILQQAFKKLSAQSTEV"
     gene            1285..1569
                     /locus_tag="O2701_002322"
     CDS             1285..1569
                     /locus_tag="O2701_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCZ4294196.1"
                     /translation="MSNLEIKQGENKFYIGDDENNALAEITYRFVDNNEINIDHTGVS
                     DELGGQGVGKKLLKAVVEHARENNLKIIASCSFAKHMLEKEDSYQDVYLG"
     gene            1740..2546
                     /locus_tag="O2701_002323"
     CDS             1740..2546
                     /locus_tag="O2701_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCZ4294197.1"
                     /translation="MAFHDKTATQVTNIVLNVRDLDLMTTFYKNILGLSVKSSDDNTT
                     VLSVGTGGHTLTLHLLEDGRQTSPREAGLFHIAFLLPTTEDLANFLYFVAQKNMGIGA
                     GDHLVSEALYFNDPEGNGIEVYRDRPSSSWEWQNGKVKMDTLEVDSQTLLTHRTDEGW
                     QGMPAKGMIGHLHLKTHDLDAAYQCYIEQLGFQHVSDFPRALFMSTNHYHHHIAANTW
                     QSNQARQNNSQSYGLTHVDIYQPDASTHTFTAPEGFDITVHSNTDLVPEK"
     gene            complement(2666..3337)
                     /locus_tag="O2701_002324"
     CDS             complement(2666..3337)
                     /locus_tag="O2701_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCZ4294198.1"
                     /translation="MSNMNQTIMDAFHFRHATKQFDPQKKVSKEDFETILESGRLSPS
                     SLGLEPWKFVVIQDQALRDELKAHSWGAAKQLDTASHFVLIFARKNVTSRSPYVQHML
                     RDIKKYEAQTIPAVEQKFDAFQADFHISDNDQALYDWSSKQTYIALGNMMTTAALLGI
                     DSCPMEGFSLDTVTDILANKGILDTEQFGLSVMVAFGYRQQEPPKNKTRQAYEDVIEW
                     VGPKE"
     gene            3607..4599
                     /locus_tag="O2701_002325"
     CDS             3607..4599
                     /locus_tag="O2701_002325"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCZ4294199.1"
                     /translation="MTKIMFFGTRDYEKEMALNWGKKNNVEVTTSKELLSSATVDQLK
                     DYDGVTTMQFGKLENDVYPKLESYGIKQIAQRTAGFDMYDLDLAKKHNIVISNVPSYS
                     PETIAEYSVSIALQLVRRFPDIERRVQAHDFTWQAEIMSKPVKNMTVAIIGTGRIGAA
                     TAKIYAGFGATITAYDAYPNKDLDFLTYKDSVKEAIKDADIISLHVPANKESYHLFDK
                     AMFDHVKKGAILVNAARGAVINTPDLIAAVNDGTLLGAAIDTYENEAAYFTNDWTNKD
                     IDDKTLLELIEHERILVTPHIAFFSDEAVQNLVEGGLNAALSVINTGTCETRLN"
     gene            complement(4848..5648)
                     /locus_tag="O2701_002326"
     CDS             complement(4848..5648)
                     /locus_tag="O2701_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCZ4294200.1"
                     /translation="MVKAIAVDMDGTFLDSKKTYDKLRFEAIFTELRNRDITFIAASG
                     NQYAKLKSIFGDRDMYFISENGAVIYNGNELYNYKSFNRQVFQQVVDYLNMKQSIDQL
                     VICGLKSAYILKHTSEAFKEDTRFYYHQLKEIDSLQQLPEDDYVKIAFNINRETHPNV
                     DEEVATQFSNDIKLVSSGNDSIDIIMPNMTKGQALKRLLDKWEMSPSELMAFGDANND
                     KDMLAFAKHSYVMENSHDEELFNIASAVAPSNDKQGVLTIIEQEVLKK"
     gene            5969..6604
                     /locus_tag="O2701_002327"
     CDS             5969..6604
                     /locus_tag="O2701_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCZ4294201.1"
                     /translation="MKLNNYSLKVKNKQLVDNCDLNFYLGQINHIVGKNGVGKSLLAK
                     DFLLNNSGNIPKSISQNVTLISSSSNIPNDITKDFLLSLLKSKFENNRQTFDKIYNIL
                     NIEAIPSNVLLKNLSDGQKQKLKLLSFLLEDHDLIILDEVTNALDKKTVNEIYEFLND
                     FIQSHQTKTIINITHNLSDLSALPGKYFIFKDLQIEEYQSKEEVINDYINL"
     gene            6610..7341
                     /locus_tag="O2701_002328"
     CDS             6610..7341
                     /locus_tag="O2701_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="HCZ4294202.1"
                     /translation="MSTLKLEFKKSISNKIIYTLVVLFTFLFLLGYFLPIGIDKVKSL
                     SYSQFFFSSYTVATQLGFLLFSFVIAYFINKEYSNKNILFYKLIGDNIFTFFYKKVAV
                     LFFECLVFIILSITIISIIYSDFSHYLLLIILFSLVILQYILVVGTISMVSPNILISL
                     GISIVYWIGSVILVAINKNIFGIVAPFEASNTMYRAVEKILNNESTFMCPTEIINIVS
                     FFVLLFIVNTIVLLLSRKRWLKIGM"
     gene            complement(7501..8121)
                     /gene="srtA"
                     /locus_tag="O2701_002329"
     CDS             complement(7501..8121)
                     /gene="srtA"
                     /locus_tag="O2701_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase SrtA"
                     /protein_id="HCZ4294203.1"
                     /translation="MKKWTNRLMTIAGVVLILVAAYLFAKPHIDNYLHDKDKDEKIEQ
                     YDKNVKEQASKDKKQQAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATPEQLNRGVS
                     FAEENESLDDQNISIAGHTFIDRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRD
                     VKPTDVGVLDEQKGKDKQLTLITCDDYNEKTGVWEKRKIFVATEVK"
     gene            8315..8806
                     /locus_tag="O2701_002330"
     CDS             8315..8806
                     /locus_tag="O2701_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCZ4294204.1"
                     /translation="MIRYAKKEDLNAILAIYNDAIINTTAVYTYEPQTIDERVAWFET
                     KQRKHEPIFVFEENGSVLGFATFGSFRPWPAYLYTIEHSIYVDASARGKGIASQLLHH
                     LIVEAKAKGYRALVAGIDASNKASIQLHQKFAFKHAGTLTNVGFKFNRWLDLAFYELD
                     LQD"
BASE COUNT         2910 a         1567 c         1346 g         3152 t
ORIGIN      
        1 gtaaacagtc tctaaaatat ttttaaagtc gtcaaaataa cttactttaa tttcaatggg
       61 gcctaatcca taaatcgctt tatctactgg aaccggtcca ttcttccaag gtacaccagg
      121 tgctacccct tcatttaact catctgaaat taattgatac acttggccat cgacttcttc
      181 aaatggcaac acttttttac caaataattc ttgaatacct tcgtgtttaa caccaaactc
      241 atcaaagcgc tgttcataat atgttaatgc gtcatcgtta ggcactctaa aagacggtct
      301 tgtaatggaa tttgttcctg cctgcccttt tgtaatattt ggaaaatcaa agaacgtcat
      361 gtctgtacct gccgaaccta catcatctgc aaaaaaagta tgatacgtat aaatatcatc
      421 ttgattgact gtctttttaa ctaaacgcat gcctagtact tctgtaaaaa atttataatt
      481 acgttctgca tcatctgtca ttgcagtaac atggtgaata cctagtaatt catgattatt
      541 tatcatttta tataccctct ttatattttt agtgcattta tctcgaaatc gagatacttt
      601 aatgtaaatt tttagtgcac gccttacact tctgtagatt gtgcacttag tttcttaaac
      661 gcttgttgta agattgttaa ttcatccttt gttaacacat caaacgcttt tgttaatgtc
      721 tcagcatgct tagggaaaat atctgccatt tgactttgac ctttttcagt taaacaagcc
      781 atatatacac gtttatcatc tttatccttt tcacgtgtaa tccaaccttt gtcctctaat
      841 tgacttacaa catatgaaat gctgctactt gcaattaata cgcggtctct aatacgttga
      901 attggttgcg gacctttatt ataaagcaac tcgagcactg caaattcagt aatatttaag
      961 ccatatcgct ttacgtcttc ttttgtaatt tgctctaatg tgtctaacgc cctattcatt
     1021 ccgacaaaaa cattgagaga ttgtttcgtt cgatccatat gatcaccaac ttttttattt
     1081 tatttcgatt tcgagataaa ttatatagct gaatagattt aatgtcaaga tacgtgtttt
     1141 catcttcatt tcaaactgtg cttaataaaa acgactacat agatgtggca atgtcgtcac
     1201 atgactgtca tgcacctttg acttcgttta agttaaaata gaatagggaa atactataat
     1261 caatcatgat aggagttgat atgtatgagt aaccttgaaa tcaaacaagg cgagaacaaa
     1321 ttctatattg gtgatgatga aaataatgct ttagctgaaa tcacataccg ttttgtggat
     1381 aataatgaaa ttaacattga tcatacaggc gtatctgatg aacttggtgg tcaaggtgtt
     1441 ggcaaaaaac tacttaaagc agttgttgaa cacgctcgag aaaataattt gaaaattatt
     1501 gcctcatgtt catttgccaa acatatgtta gaaaaagaag attcatatca agatgtatat
     1561 cttggttaaa taaattttgc gatggtgcaa ctgtaccatc gcttttttgt tcgcatcaat
     1621 ataaaattgc aaaacgacta gttattttgc atttcaaact ttatgctatc aattcatttt
     1681 cttttctggt atacttttta tactaactta tagaaaaata gaaaaagagg cgatgcataa
     1741 tggcatttca cgataaaaca gcaacacaag tgacaaacat tgtactgaac gtaagagact
     1801 tagacttaat gacaacattt tacaaaaaca ttttaggact atccgttaaa agttctgacg
     1861 ataatacaac cgtattatct gttgggactg gtggccatac tctaacgtta catttattag
     1921 aagacggccg tcagacttcc ccacgtgaag cagggctttt tcatatagca tttttattac
     1981 caactactga ggatctagct aacttcttat atttcgtggc acaaaaaaat atgggcatcg
     2041 gcgctggtga tcatttagta agtgaagctt tatatttcaa cgaccccgaa ggtaatggta
     2101 ttgaagtcta tcgcgataga ccttcatctt catgggaatg gcaaaatggc aaagttaaaa
     2161 tggatacttt agaagttgat agccaaacct tattaacaca tcgtactgat gaaggttggc
     2221 aaggaatgcc agcaaaaggc atgattggac acttacattt aaaaacacat gatttagacg
     2281 cagcatatca atgttacatt gaacaattag gattccaaca tgtgtctgat tttccacgtg
     2341 cgctatttat gtcaacgaat cattatcacc atcatattgc tgctaatact tggcaatcca
     2401 atcaagcaag acaaaacaat tcacaaagtt atggattaac acatgttgat atttatcaac
     2461 ccgacgcctc aacacataca tttacggcac cagaagggtt tgacatcaca gttcattcaa
     2521 atactgacct tgttccagaa aaataatcat ccatacttta ttaatacaat tttttcacgc
     2581 aaaagcctgc gattcattta tgtaatcgca ggctttttta ttgctaactt tttaatttta
     2641 tatatttaga catacggtat tctatttatt cttttggtcc aacccattca ataacatctt
     2701 cataagcttg gcgtgtttta tttttcggtg gctcttgttg tctgtagcca aatgcgacca
     2761 tcactgataa accaaattgc tcagtatcta agatcccttt atttgctaaa atgtctgtca
     2821 ctgtatccag actaaaacct tccatcggac atgaatcaat acctaacaat gcggctgtcg
     2881 tcatcatatt gcctaatgca atatacgttt gtttacttga ccagtcatac aaggcttgat
     2941 cattatcaga aatatggaaa tctgcttgga atgcatcgaa tttttgttca acagctggaa
     3001 tcgtttgtgc ctcatatttt ttaatatctc ttaacatatg ttgtacatac ggtgatcttg
     3061 acgttacatt tttacgcgca aaaattagca caaaatggct cgctgtatct aattgttttg
     3121 ctgcgcccca actgtgcgct tttaattcat cacgtaacgc ttgatcttga atcacgacaa
     3181 acttccaagg ttctaaccca agagaacttg gagacaatct acctgactct aatattgttt
     3241 caaaatcttc tttcgaaact ttcttttgtg gatcgaattg cttagtcgca tgtctgaaat
     3301 gaaatgcatc cataattgtt tgattcatat tgctcattga acatagcctc ccatttttat
     3361 atttattgct atacaaatta tacttcatgt tttaaaaaga ctaaagtaat ccgtttgcaa
     3421 ttcactataa cttgtgcaca tgttataagt tttctgcaat aaaacacact tttgaatttt
     3481 gggaaattta gtgttataat atatttgtga tatttttcac aattaaattg tggcgtgatt
     3541 ttaatgtgca taatctttaa tttcactcat ttacttttca atcttttaaa agcgaggttt
     3601 ttaataatga caaaaattat gttctttggt acgcgtgatt atgagaaaga gatggcatta
     3661 aattggggga aaaagaataa tgtcgaagta actacttcta aagagctatt atcaagtgct
     3721 acagtcgatc aattaaaaga ttacgatggc gtaactacaa tgcaatttgg taagttagaa
     3781 aatgacgttt atcctaaatt agaatcttac ggtattaaac aaattgcaca acgtactgct
     3841 ggatttgata tgtatgattt agatttagct aaaaaacaca atattgtgat atctaacgtt
     3901 cctagttatt cacctgaaac aattgcagag tattctgttt ctatcgccct acaattagtg
     3961 cgtcgcttcc cagatattga acgccgtgta caagcacatg attttacttg gcaagcagaa
     4021 atcatgtcta aaccagttaa aaatatgact gttgcaatta ttggtacggg tcgtatcggt
     4081 gctgctacag ctaaaatata tgcaggattt ggtgctacaa ttacagctta tgacgcctat
     4141 cctaataaag atttagactt tttaacttat aaagatagtg ttaaagaagc tattaaagat
     4201 gccgatatta tttctttaca tgttccagcg aacaaagaaa gctatcattt attcgataaa
     4261 gcaatgtttg atcatgtgaa aaaaggtgca atcttagtta acgcagcacg tggtgcagtc
     4321 atcaatacac ctgatttaat cgctgcagtg aacgatggta ctttgttagg tgctgcgatt
     4381 gatacttatg aaaatgaagc agcatacttc acaaatgact ggactaataa agacattgac
     4441 gataaaacat tattagagtt aatcgaacat gaaagaattt tagtaacacc acatattgct
     4501 ttcttctctg atgaagcagt acaaaacctt gttgaaggtg gtttaaacgc agcattatct
     4561 gtcattaaca ctggtacatg tgaaacacgt ttaaattaat atatatattt aaaagcaaag
     4621 gcgcgccatg tgcctttgct ttttattttg tatatctgta tcaaaatcga tttgattaaa
     4681 atccgcttta ttttatcatc tattcaaatg attttagtgc gcttatttta ctatggcatt
     4741 attgcctaac ctgtttgaga tatattaata tttgatgatt cgtattcaaa gttttcattt
     4801 aacatgtatt tagtatcatg atagctgctt cattgatgat atactaccta ctttttcaac
     4861 acttcttgtt cgataatcgt taatacaccc tgcttatcat tacttggagc gacagcagag
     4921 gcaatattga acaattcttc atcatgacta ttttccatca cataactatg cttagcgaac
     4981 gctaacatat ctttatcatt attcgcatct ccgaaggcca tgagctctga aggagacatt
     5041 tcccatttat ctaacaatcg ttttaatgcc tgccctttag tcatgtttgg cataataata
     5101 tcaatgctat cgttcccact tgagacaagt ttaatatcat tgctgaattg cgttgctact
     5161 tcttcgtcaa cattcggatg cgtctcacga ttaatattaa atgctatttt gacataatca
     5221 tcctcaggta attgctgtag actgtcaatt tcttttaact gatgataata aaatctcgta
     5281 tcttctttaa acgcttcaga agtatgtttt aaaatatacg cgcttttcaa accacagatg
     5341 acgagttgat caatactttg cttcatattt aagtaatcga caacctgttg aaacacctga
     5401 cgattaaagc ttttataatt atataactca ttgccattat aaataactgc accattttca
     5461 gaaataaaat acatatctct atccccgaaa atagacttca acttcgcata ttgattgcca
     5521 ctcgcagcaa taaatgtaat atctctattt ctaagttcag taaaaatcgc ttcaaatcta
     5581 agtttatcat atgtcttttt tgaatctaaa aacgtcccgt ccatatctac cgcaatagcc
     5641 ttaaccatga ttatcctcct cattgctctt ttagtcgtct tcattatatc catctcatga
     5701 caagcaatga agtttgtcat accacaatga acctatattc acattttgaa attgctttca
     5761 tatatgcaaa acacattaca ctggatatag cattcagagg cgaacataaa tttcagaata
     5821 ttgtaaaaat atcacactat atttatcatt tagacaaaat atagtgtgat atactaattt
     5881 ataacatcta tacataatgt gaaagaattt ttaaaaatac aattaaatat cggtgtaata
     5941 aatcccttta tacataggag ttgtatacat gaaactaaat aactattctt taaaagttaa
     6001 aaacaaacaa ctagttgaca attgtgattt aaatttctat cttggtcaga tcaatcacat
     6061 tgttggtaaa aatggtgtag gaaaatcttt attagctaaa gatttcttac taaataatag
     6121 tggaaatatc cctaagtcca tttctcaaaa tgtaacctta atatcaagtt catcaaatat
     6181 tcctaatgat ataacaaaag attttttatt atcattgtta aaatcaaaat ttgaaaacaa
     6241 tcgacaaaca ttcgataaga tttataacat actaaacatc gaagcaatac cgtctaacgt
     6301 actactaaaa aacttgagtg atggtcaaaa acaaaagctt aaattattaa gtttcttatt
     6361 ggaagatcat gatttaatca tattagatga agttacaaac gctttagaca agaaaacagt
     6421 taatgaaatt tatgaatttt taaatgattt tattcaaagc catcaaacta aaactattat
     6481 caatattaca cataatttat ccgatttaag tgctttgccg ggtaaatact ttatttttaa
     6541 agaccttcaa atagaagagt accaatcaaa agaagaagtc ataaatgatt acattaattt
     6601 ataattgagg tgtccacttt gaaattagaa tttaaaaaaa gtatttccaa taaaattatt
     6661 tacacgcttg ttgtgctatt cacattcctt ttcttacttg ggtatttttt accaataggt
     6721 atagacaaag ttaaaagttt aagctatagc cagtttttct tcagttcata tactgttgca
     6781 acgcaattag ggttcttact attttcattt gttattgcat attttattaa taaagagtat
     6841 tcgaataaaa atatcctgtt ttataaattg attggcgata atatctttac cttcttctat
     6901 aaaaaggtag cagtgttatt ttttgaatgt ttagtgttca taattctaag tatcactatt
     6961 atttcaatta tatattcgga tttctcacat tacttactat taataatctt attttcactc
     7021 gttatcttac aatatatctt agttgttgga acgattagta tggtttcacc taacatttta
     7081 atcagcttag gtatcagcat tgtatattgg attggttcag tcatcttagt tgccattaat
     7141 aaaaatatat ttggtatcgt tgctccattt gaggcgagca atacgatgta tcgtgccgta
     7201 gaaaaaatat tgaataacga gtcaacattt atgtgtccta ctgaaattat aaatatagta
     7261 tcattcttcg ttcttctatt tatagttaat actattgtct tattactaag tagaaagaga
     7321 tggttgaaaa tcggtatgta gtataaaaaa ggagcggtat acattgccgc ttctttttat
     7381 tatgtatttt tacaatctcc accaatgaca cgtcgtcatt gctacctcat accaaacaaa
     7441 aaacagtctc gcaatcaaga ctgttttcca ctcaatatat tcatccatta gcgtaataga
     7501 ttatttgact tctgtagcta caaagatttt acgtttttcc caaacgcctg tcttttcatt
     7561 gtaatcatca caagtaatta atgttaattg tttatcttta cctttttgtt catctagaac
     7621 tcctacatct gtaggcttaa catctcttat acttgtcatt ttatacttac gtgtttcatt
     7681 accaacttta aagtacacca tactaccttt tttggctgct ttaagatttg taaattgata
     7741 gttcggacgg tcaatgaaag tgtgtcctgc aattgaaata ttttgatcat ctagtgattc
     7801 attttcttct gcaaagctta cacctctatt taattgttca ggtgttgctg gtcctggata
     7861 tactggttct ttaatatcag catctggaat ttcaatatag cctgccactt tcgatttatc
     7921 tttcggaatt tgaggtttag cttgctgctt tttatcttta ctcgcctgtt cttttacatt
     7981 tttatcatat tgttcaatct tttcatcttt atctttatcg tgaagataat tatcgatatg
     8041 tggtttagca aacaaatatg ctgccactag gataagtacc acaccagcga ttgtcattaa
     8101 tcgatttgtc cattttttca tacgttaagg ctccttttat acatttcaca atgcctgtta
     8161 acatttcata tagtatacca ttaatttcaa aatgactcat agcaattcat tttatactat
     8221 aaaatttaca tgtatacttt tacgttagat ttcattacac atatttgcat tcaaataacg
     8281 aaacgctttt aataattact aagggggaat tgatatgatt agatacgcta aaaaagagga
     8341 tttaaacgct atattagcga tatacaatga tgccattatc aatactacag ctgtttatac
     8401 ttatgaacca caaaccatag acgaacgtgt cgcatggttt gaaacgaaac aacgtaagca
     8461 tgagcctatc tttgtatttg aggaaaatgg aagtgtctta gggtttgcga cgttcggttc
     8521 atttagacct tggccagcat acctatatac aatcgaacat tctatttatg tcgatgcttc
     8581 agctagagga aaaggtattg ctagtcaatt actacaccat ttaattgtgg aagcaaaagc
     8641 taaaggttat cgtgcgctag ttgcaggcat tgatgcttcc aacaaagcga gtattcagtt
     8701 gcatcaaaag tttgctttta agcatgccgg cacactgacc aatgtaggtt ttaaatttaa
     8761 tagatggtta gatttagcat tttacgaatt agatttacaa gactagtaat gtttgaatca
     8821 cataatataa acaagacaac catgttaatt cccttaacat aacaagccaa catataaaat
     8881 tttaaacttc tcaggggagt gggacagaaa tgataaagag ccactaatga tttattatgt
     8941 agtggttctt acacattagc cacagctaat gtgta
//
LOCUS       DALAGD010000107         8832 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.108, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000107 DALAGD010000000
VERSION     DALAGD010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8832
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.108"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            261..1217
                     /locus_tag="O2701_002331"
     CDS             261..1217
                     /locus_tag="O2701_002331"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCZ4294205.1"
                     /translation="MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYKGP
                     DMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
                     GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQL
                     YQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLA
                     RESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
     gene            1417..1896
                     /locus_tag="O2701_002332"
     CDS             1417..1896
                     /locus_tag="O2701_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCZ4294206.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            1911..2750
                     /gene="fakB2"
                     /locus_tag="O2701_002333"
     CDS             1911..2750
                     /gene="fakB2"
                     /locus_tag="O2701_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HCZ4294207.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTYNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSIGVAHANVIEYVDKLKKVFNEAFHVNNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            2836..3369
                     /gene="msrA"
                     /locus_tag="O2701_002334"
     CDS             2836..3369
                     /gene="msrA"
                     /locus_tag="O2701_002334"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCZ4294208.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHVDNPTYE
                     QVCTNQTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DEHQKKAAEFKKQQLNEQGIFKKPVITPIKPYKNFYPAEDYHQDYYKKNPVHYYQYQR
                     GSGRKAFIESHWGNQNA"
     gene            3362..3790
                     /gene="msrB"
                     /locus_tag="O2701_002335"
     CDS             3362..3790
                     /gene="msrB"
                     /locus_tag="O2701_002335"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCZ4294209.1"
                     /translation="MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKIS
                     GKPLFTSEEKFHSECGWPSFSKALDDDEIIELVDKSFGMLRTEVRSEESNSHLGHVFN
                     DGPKESGGLRYCINSAAIQFIPYEKLEELGYGDLISHFDK"
     gene            3802..4302
                     /locus_tag="O2701_002336"
     CDS             3802..4302
                     /locus_tag="O2701_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCZ4294210.1"
                     /translation="MFKKLFGKGKEVQKDIAIYAPLTGEFVKIEDIPDPVFAQKMMGE
                     GFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNGLELLVHIGLDTVQLDGEGFEVL
                     VSSGDEVNVGDPLVRFNLEYINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKV
                     IDVTMN"
     gene            4302..4523
                     /locus_tag="O2701_002337"
     CDS             4302..4523
                     /locus_tag="O2701_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HCZ4294211.1"
                     /translation="MKNYSFYQFVMTVRGRHDDKGRLAEEIFDDLAFPKHDDDFNILS
                     DYIETHGDFTLPMSVFDDLYEEYTEWLKF"
     gene            4715..6205
                     /locus_tag="O2701_002338"
     CDS             4715..6205
                     /locus_tag="O2701_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HCZ4294212.1"
                     /translation="MDDKQHTSSSDDERAEIATSNQDQETNSSKRVHLKRWQFISILI
                     GTILITAVITVVAYIFINQKISGLNKTDQANLNKIENVYKILNSDYYKKQDSDKLSKA
                     AIDGMVKELKDPYSEYLTKEQTKSFNEGVSGDFVGIGAEMQKKNDQIMVTSPMKGSPA
                     ERAGIRPKDVITKVNGKSIKGKALDEVVKDVRGKENTEVTLTVQRGSEEKDVKIKREK
                     IHVKSVEYKKKGKVGVITINKFQNDTSGELKDAVLKAHKDGLKKIVLDLRNNPGGLLD
                     EAVKMANIFIDKGKTVVKLEKGKDTEAIQTSNDALKEAKDMDISILVNEGSASASEVF
                     TGALKDYNKAKVYGSKTFGKGVVQTTREFKDGSLLKYTEMKWLTPDGHYIHGKGIKPD
                     VTIDTPKYQSLNVIPNTKTFKVGDDDKNIKTIKIGLSALGYKVDNESTQFDKALENQV
                     KAFQQANKLEVTGEFNKETNNKFTELLVEKANKHDDVLDKLINILK"
     gene            6605..7114
                     /locus_tag="O2701_002339"
     CDS             6605..7114
                     /locus_tag="O2701_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4294213.1"
                     /translation="MIRLGKMSDLDQILNLVEEAKELMKEHDNEQWDDQYPLLEHFEE
                     DIAKDYLYVLEENDKIYGFIVVDQDQAEWYDDIDWPVNREGAFVIHRLTGSKEYKGAA
                     TELFNYVIDVVKARGAEVILTDTFALNKPAQGLFAKFGFHKVGEQLMEYPPYDKGEPF
                     YAYYKNLKE"
     gene            7126..8196
                     /locus_tag="O2701_002340"
     CDS             7126..8196
                     /locus_tag="O2701_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCZ4294214.1"
                     /translation="MTKIAFTGGGTVGHVSVNLSLIPTALSQGYEALYIGSKNGIERE
                     MIESQLPEIKYYPISSGKLRRYISLENAKDVFKVLKGILDARKVLKKEKPDLLFSKGG
                     FVSVPVVIAAKSLNIPTLIHESDLTPGLANKIALKFAKKIYTTFEETLNYLPKEKADF
                     IGATIREDLKNGNAHNGYQLTGFNENKKVLLVMGGSLGSKKLNSIIRENLDALLQQYQ
                     VIHLTGKGLKDAQVKKSGYIQYEFVKEDLTDLLAITDTVISRAGSNAIYEFLTLRIPM
                     LLVPLGLDQSRGDQIDNANHFADKGYAKAIDEEQLTAQILLQELNEMEQERTRIINNM
                     KSYEQSYTKEALFDKMIKDALN"
     gene            8213..8827
                     /locus_tag="O2701_002341"
     CDS             8213..8827
                     /locus_tag="O2701_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCZ4294215.1"
                     /translation="MSQWKRISLLIVFTLVFGIIAFFHESRLGKWIDNEVYEFVYSSE
                     SFITTSIMLGATKVGEVWAMLCISLLLVAYLMLKRHKIEALFFALTMALSGILNPALK
                     NIFDRERPTLLRLIDITGFSFPSGHAMGSTAYFGSGIYLLNRLNQGNSKGILIGLCAA
                     MILLISISRVYLGVHYPTDIIAGIIGGLFCIILSTLLLRNKLIN"
BASE COUNT         3298 a         1207 c         1578 g         2749 t
ORIGIN      
        1 tattcaaatc atgtgttaac ctctttttta aaatttttta gcattaaagt tatagatttg
       61 ggtaaacaat taccaattgg aaacatatat cacgttacga tggggtaggt acttaatcag
      121 cattttataa ataaagtaac ggaattcatg atattaatat catattccta aaatgagtga
      181 taacaaaatg ctacataaag ttaagttata tcaaactaaa tatacatact ataaataatg
      241 aaaatgaggt gttatcgcat atgttgaatt catttgatgc agcatatcac agtctttgtg
      301 aagaagtttt agaaatagga aatacacgaa atgatcgcac aaatacaggt acgatttcga
      361 aatttggtca tcaacttcgc tttgacttat ctaaaggatt tccactatta acgacaaaga
      421 aagtttcttt taaattagta gcaaccgaat tattatggtt cattaaagga gatacaaaca
      481 tccaatactt attaaaatat aataataata tatggaacga atgggctttt gaaaattata
      541 tcaaatcaga cgagtataaa ggtccagata tgacagattt cgggcatcgt gcattgagtg
      601 atcctgaatt taacgaacaa tataaagaac aaatgaaaca atttaagcaa cgtattcttg
      661 aagatgatac atttgcgaag caattcgggg atttaggaaa tgtttatggt aaacaatggc
      721 gagattgggt tgataaagat ggtaatcatt ttgatcaact taaaacagta attgaacaaa
      781 ttaagcataa tccagattca aggcgacaca tcgtatctgc atggaatcca acagaaattg
      841 atacaatggc acttccgcct tgtcatacca tgttccagtt ttatgtccaa gatggtaagt
      901 taagttgcca gttataccaa cgtagcgcag atatcttttt aggtgtgcca tttaatatcg
      961 caagctacgc tttattgaca caccttattg ccaaagaatg tggacttgaa gtgggtgaat
     1021 ttgtgcatac atttggagat gcacatattt attcaaatca tattgatgcg attcaaacac
     1081 aattagcacg tgaaagcttc aatcctccaa cattaaaaat taacagtgac aagtctattt
     1141 tcgacataaa ttatgaagat ttggaaattg ttgactatga atcacatcca gcaataaaag
     1201 ctccaatagc agtgtagtca ttgcatagtt agctaaccat atagacatca aaatgacatc
     1261 atagtatttt caagtgcaaa aaagtacttt tttgtgttaa acgttttcat aaattatgca
     1321 aaatcattat ttctatcaca ctttatgata aaaattgtgt taaattaaag ataacttagt
     1381 aataaaaaat gaaatgatag aagaaggagg ataattatga ctttatccat tctagttgca
     1441 catgacttgc aacgagtaat tggttttgaa aatcaattac cttggcacct accaaatgat
     1501 ttgaagcatg ttaaaaaatt atcaacaggt catactttag taatgggtcg taagacattt
     1561 gaatcgattg gtaaaccact accgaatcgt cgaaatgttg tacttacttc agatacaagt
     1621 ttcaacgtag agggcgttga tgtaattcac tctattgaag atatttacca actaccgggc
     1681 catgttttca tatttggagg gcaaacatta tttgaagaaa tgattgataa agtggacgac
     1741 atgtatatta ctgttattga aggtaaattc cgtggtgata cgttctttcc accttataca
     1801 tttgaagact gggaagttgc ctcttcagtt gaaggtaaac tagatgagaa aaatacaatt
     1861 ccacatacct ttctacattt aattcgtaaa aaataagggg gaaaacgacc atgacaaaac
     1921 agattatagt aacagactca acatccgatt tatctaaaga atacttagaa gcaaacaaca
     1981 ttcatgtaat tcctttaagt ttaactattg aaggagcttc atacgttgac caagtagata
     2041 ttacatcaga agaatttatt aatcatattg aaaatgatga agatgtaaag acaagtcagc
     2101 cagccatagg tgaatttata tctgcttatg aagaactagg aaaagatggc tctgaaatca
     2161 taagtattca tctttcttca ggattaagtg gtacatataa cactgcttac caagcaagtc
     2221 aaatggtaga tgctaatgta actgttattg attcaaaatc tatttctttt ggtttagggt
     2281 atcaaataca acacctagta gagcttgtaa aagaaggtgt ctcaacttct gaaatagtta
     2341 aaaagttaaa tcatttaaga gaaaacatta aattatttgt agttataggg caattgaatc
     2401 aattaattaa aggtggcaga attagtaaaa caaaaggttt gattggtaat cttatgaaaa
     2461 ttaaaccaat tggtacacta gatgatggtc gcttagagct tgtgcacaat gcgagaactc
     2521 aaaattctag tatccaatac ttgaaaaagg aaattgctga atttatagga gatcatgaaa
     2581 tcaaatccat tggtgtcgca catgctaacg tcattgaata tgttgataaa ttgaagaaag
     2641 tttttaatga agcttttcat gtgaataatt acgatataaa tgtaactaca ccagttattt
     2701 ctgcacatac tggtcaaggt gcgattggcc tcgtagtcct taagaagtaa atttaatctt
     2761 ttcagtgtta attacttcca tttcaatcct ttatagacta aatttataat tagatagata
     2821 gaggaggtaa ttcatatgac aaaagaatat gcaacattag caggaggatg tttctggtgc
     2881 atggttaaac catttacatc atatccaggc atcaagtcag tcgtatctgg ttatagtggc
     2941 ggtcatgttg acaacccaac ttatgaacag gtatgtacga atcaaaccgg ccatgtcgaa
     3001 gcagtacaaa ttacgtttga tccagaggtt acttcctttg aaaatatatt agacatatat
     3061 ttcaaaacat ttgacccaac tgatgatcaa gggcaatttt tcgatagagg cgaaagctat
     3121 caaccagtca ttttctatca tgatgaacat cagaaaaagg ctgctgagtt taaaaagcaa
     3181 caattaaatg aacaaggtat tttcaagaaa ccagtgatta cacctattaa accatataaa
     3241 aatttctatc cagctgaaga ctaccatcaa gattattaca aaaagaaccc ggtacattat
     3301 taccaatatc aacgtggttc aggtagaaaa gcgtttatag aatcacattg ggggaatcaa
     3361 aatgcttaaa aaagataaaa gtgaactaac agatatagaa tatattgtta cacaagaaaa
     3421 cggcactgaa ccaccattta tgaatgaata ttggaatcat tttgctaaag gaatttatgt
     3481 agataaaatt tctggtaaac ctttatttac atctgaagaa aagtttcatt ctgaatgtgg
     3541 atggcctagc ttttccaaag cgcttgatga cgatgaaatt atagaattag tcgacaaatc
     3601 atttggtatg ttgagaactg aagtgcgttc agaagaatca aatagtcatt taggacatgt
     3661 ctttaatgat ggacctaaag aaagtggcgg cttaagatac tgtatcaatt ccgctgcaat
     3721 tcaatttatt ccatatgaaa agttagaaga attgggttat ggcgatttaa tatcacattt
     3781 tgataagtag gagtggaaag tatgtttaaa aaattattcg gaaaaggcaa agaagttcag
     3841 aaagatatcg caatttatgc accattaact ggagaattcg tgaaaattga agatattcca
     3901 gatcctgtat tcgcacaaaa aatgatgggc gaaggttttg gtattaatcc aactgaagga
     3961 gaagttgtgt ctccaattgc aggacgtgtt gacaatgtct ttccaactaa gcatgcgatt
     4021 gggttaaaag cagataacgg attagaatta ttagttcata tcggtttaga cacagttcaa
     4081 ttagacggtg aaggatttga agtgttagtg tctagtggtg acgaagttaa cgtcggagat
     4141 ccattagtaa gattcaacct tgaatatatt aataataacg ctaaatctgt aatttcacca
     4201 attataatta ctaacactga tcaagcagct tcaattaata tttatgatga aaatgctgtg
     4261 attaaaggtg aaacaaaagt gattgatgtg acaatgaact aatgaaaaac tattcgtttt
     4321 atcaatttgt catgacagtt cgtggtcgac acgacgataa aggtcgtcta gcagaagaga
     4381 tatttgacga tcttgctttc ccaaaacacg atgatgattt taacatactg tctgattata
     4441 ttgagacaca tggtgatttc acattgccaa tgtctgtatt tgatgattta tatgaagaat
     4501 atacggaatg gctaaaattt taatataatt tttaataata atagttagaa ccagggtgat
     4561 gcaattcgtt atcctggttt taatttaaaa taaactaagt tgtgactaaa aattaatcaa
     4621 ttatagtgaa atatggtgcg ctatcttgca taaattgata tgattaacta cacagaattt
     4681 aaaagtacat aatacataat aaggaagtga tacaatggat gataagcaac acacatcttc
     4741 atccgatgat gaacgcgctg aaattgcaac aagcaatcaa gaccaagaaa ctaattcatc
     4801 gaaacgcgtt cacttaaaac gttggcaatt catatcaata ttaattggta caatcctaat
     4861 aacagctgtc atcacagttg ttgcatatat ttttataaat caaaaaataa gtggtttaaa
     4921 caaaactgat caagcaaact taaataaaat tgaaaatgtg tataaaatct taaatagtga
     4981 ttattacaaa aaacaggact ctgacaagtt aagtaaagct gcaattgatg gcatggtcaa
     5041 agaattaaaa gatccttatt ctgaatattt aacaaaagaa caaacgaaat cctttaatga
     5101 aggtgtttca ggtgattttg taggtattgg tgcagaaatg caaaagaaaa atgatcaaat
     5161 tatggttact agtcctatga agggatctcc agcagaacgt gctggcattc gtcctaaaga
     5221 tgtcattact aaagtaaatg gaaaatcaat taaaggtaaa gcattagatg aagttgtcaa
     5281 agatgttcgt ggtaaagaaa acactgaagt cactttaact gttcaacgag gtagtgaaga
     5341 aaaagacgtt aagattaaac gtgaaaaaat tcatgttaaa agtgttgagt ataagaaaaa
     5401 aggtaaagtt ggagttatta ctattaataa attccagaat gatacatcag gtgaattgaa
     5461 agatgcagtt ctaaaagctc acaaagatgg tttgaaaaag attgttttag atttaagaaa
     5521 taatccaggt ggactactag atgaagctgt taaaatggca aatattttta tcgataaagg
     5581 aaaaactgtt gttaaactag aaaaaggtaa agatactgaa gcaattcaaa cttctaatga
     5641 tgcgttaaaa gaagcgaaag acatggatat atccatctta gtgaatgaag gttcagctag
     5701 cgcttctgaa gtgtttactg gtgcgctaaa agactataat aaagctaaag tttatgggtc
     5761 aaaaacattc ggcaaaggtg tcgtacaaac tacaagagag tttaaggatg gttcattgtt
     5821 aaaatatact gaaatgaaat ggttaacgcc agatggtcat tatattcacg gtaaaggcat
     5881 taaaccagac gttactattg acacacctaa atatcaatct ttaaatgtca ttcctaatac
     5941 gaaaacattt aaagttggag acgatgataa aaatattaaa actattaaaa ttggtttatc
     6001 agctttaggt tataaagttg ataatgaatc aacgcaattt gataaagctt tagaaaatca
     6061 agttaaagct ttccaacaag cgaataaact tgaggtaact ggtgaattta ataaagaaac
     6121 gaataataaa tttactgagt tattagttga aaaagctaat aaacatgatg atgttctcga
     6181 taagttgatt aatattttaa aataagcgat acacactact aaaattgtat tattattatg
     6241 ttaatgacac gcctcctaaa tttgcaaaga tagcaattta ggaggcgtgt ttatttttat
     6301 tgacgtctaa ctctaaaaga tataaattag acatttacaa atgatgtaaa taacgcaatt
     6361 tctatcatcg ctgataacaa ttcatggttt aatatgcaat gagcatatac tttttaaata
     6421 gtattattca ctagttttaa caatcaatta attggtatat gatactttta ttggttattt
     6481 ttatcccata gtgtgataat tactattttt cattcataat aaaggtttaa agcatgttaa
     6541 tagtgtgtaa gattaacatg tactgaaaaa catgtttaac ataatgatat aaggagtgac
     6601 gtacatgatc cgtctaggta aaatgtcaga tttagatcaa atcttaaatc tagtagaaga
     6661 agcaaaagaa ttaatgaaag aacacgacaa cgagcaatgg gacgatcagt acccactttt
     6721 agaacatttt gaagaagata ttgctaaaga ttatttgtac gtattagagg aaaatgacaa
     6781 aatttatggc tttattgttg tcgaccaaga ccaagcagaa tggtatgatg acattgactg
     6841 gccagtaaat agagaaggcg cctttgttat tcatcgatta actggttcga aagaatataa
     6901 aggagctgct acagaattat tcaattatgt tattgatgta gttaaagcac gtggtgcaga
     6961 agttatttta acggacacct ttgcgttaaa caaacctgca caaggtttat ttgccaaatt
     7021 tggatttcat aaggtcggtg aacaattaat ggaatatccg ccatatgata aaggtgaacc
     7081 attttatgca tattataaaa atttaaaaga atagaggtaa tattaatgac gaaaatcgca
     7141 tttaccggag ggggaacagt tggacacgta tcagtaaatt taagtttaat tccaactgca
     7201 ttatcacaag gttatgaagc gctttatatt ggttctaaaa atggtattga aagagaaatg
     7261 attgaatcac aactaccaga aattaagtat tatcctattt cgagtggtaa attaagaaga
     7321 tatatttctt tagaaaatgc caaagacgta tttaaagtat tgaaaggtat tcttgatgct
     7381 cgtaaagttt tgaaaaaaga aaaacctgat ctattatttt caaaaggtgg atttgtatct
     7441 gtgcctgttg ttattgcagc caaatcatta aatataccaa ctcttattca tgaatctgac
     7501 ttaacaccag gattagcgaa taagatagca cttaaatttg ccaagaaaat atatacaaca
     7561 tttgaagaaa cgctaaacta cttacctaaa gagaaagctg attttattgg agcaacaatt
     7621 cgagaagatt taaaaaatgg taatgcacat aatggttatc aattaacagg ctttaatgaa
     7681 aataaaaaag ttttactcgt tatgggtgga agcttaggaa gtaaaaaatt aaatagcatt
     7741 attcgcgaaa acttagatgc attattacaa caatatcaag tgatacattt aactggtaaa
     7801 ggattaaaag atgctcaagt taaaaaatca ggatatatac aatatgaatt tgttaaagag
     7861 gatttaacag atttattagc aattacggat acagtaataa gtagagctgg atcaaatgcg
     7921 atttatgagt tcttaacatt acgtatacca atgttattag taccattagg tttagatcaa
     7981 tcccgaggcg accaaattga caatgcaaat cattttgctg ataaaggata tgctaaagcg
     8041 attgatgaag aacaattaac agcacaaatt ttattacaag aactaaatga aatggaacag
     8101 gaaagaactc gaattatcaa taatatgaaa tcgtatgaac aaagttatac gaaagaagct
     8161 ttatttgata agatgattaa agacgcattg aattaatggg gggtaatgct ttatgagtca
     8221 atggaaacgt atctctttgc tcatcgtttt tacattggtt tttggaatta tcgcgttttt
     8281 ccacgaatca agacttggga aatggattga taatgaagtt tatgagtttg tatattcatc
     8341 agagagcttt attacgacat ctatcatgct tggggctact aaagtaggtg aagtctgggc
     8401 aatgttatgt atttcattac ttcttgtggc atatctcatg ttaaagcgcc acaaaattga
     8461 agcattattt tttgcattaa caatggcatt atctggaatt ttgaatccag cattaaaaaa
     8521 tatattcgat agagaaagac ctacattgct gcgtttaatt gatataacag gatttagttt
     8581 tcctagcggt catgctatgg gatcaactgc atattttgga agtggtatct atctattaaa
     8641 tcgattaaat caaggtaatt caaaaggtat tcttataggg ttatgtgcag ctatgatttt
     8701 attgatttcc atatcacgtg tatatctagg tgtacattat ccaacagata ttattgccgg
     8761 cattattggt ggattatttt gcattatttt atcaacgtta ttacttagaa ataaattaat
     8821 aaattaaata gt
//
LOCUS       DALAGD010000108         8592 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.109, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000108 DALAGD010000000
VERSION     DALAGD010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8592
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.109"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..2215
                     /locus_tag="O2701_002342"
     CDS             <1..2215
                     /locus_tag="O2701_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HCZ4294216.1"
                     /translation="KIREIQKLILAYEKTEIGFGEELYKDIIHHMKALFKCKNYLQID
                     TKIDMINNYLHQDIATNISEAAYLLWLLSRNNIGFTDLKVLHNRFIEKYGFEQLVNIK
                     DLISDITGFGTTIFQEEETDGNNIVMLKQKFLHALRNNDEIVINEKDVESLINDNEIN
                     HYHAPMSADVYAELYLGRFYNQYNELIVISPLTASFNAGATFGRFHHLIDTETLAKLE
                     HEKGHYYQKMICDDNVEMISINNIPKYPRNHNVLTNHDSYEYSLNLGSSNSYSKYELT
                     LDDIYVGATFNKLYLYSSQLNKRVLFESNNMYNFLKECNLYRLLREISMESVKCIEPM
                     NDVSIDSFSYSPRIRYKNVILKPAYWKINEMVLPLPKNEEWDQQFLKYQEQFNIPNIV
                     NLVYGDNKLLLNLSLANHRYLLMKEYKKHKRVRLVESFLPQSKNDHVYEIVTPIYKKS
                     SYRGPEIEIPKYKNTDIEYDKDWFALHIHIDKPSQDTFIIDNLYPFVKHLKDKGDIDQ
                     YFLMRYIKQGDILKLRLFRNDENYAEIYSILKNWLPHVRQTTEVSDYEFVSYEPEFFR
                     YGGKNTINEIEAFFEYDTNLAVNIIENDFKFDRPYIVAISIMYLFEMFSISNEERMEI
                     VNNYVPTSFKSKDIRPFKNELVTICNPANNFEYMAKHYSGIYRILKDGNQILSKLNEG
                     LKKTLTTKRSRIIGSLIHMRCNRIFGIDKDQETFVLSIVKEIVKTQKHWCGDKND"
     gene            2208..3452
                     /locus_tag="O2701_002343"
     CDS             2208..3452
                     /locus_tag="O2701_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase C family protein"
                     /protein_id="HCZ4294217.1"
                     /translation="MIDMNLILKKKFQEISEVDDFISKASTETDYFEPSTLSHGIPGI
                     ILFLDAYQKVFDINTEQIVHKYIMKLAPYLQSGQYNNSLFGGLSGIAFSMDLASQNGK
                     NYQNILNNIDGHIVNEIENKFDQILQEPLNPLNYDTVSGLAGIGRYLLNRVDENEFNV
                     KALKSILVYFKDIQYSKNSWVVPQESQFLESDKNYFTEGNINLGLAHGVLGPMSLFAL
                     CVIKGITIENHQHILKDMYKFIMDEKFCNHERWLQRYDLISERNHFNFIRNGWCYGNT
                     GVMTTLFLIGQALQDDEIIKMSKKVMLQVVNDKDENLISPTICHGLSSQILMLTIMNL
                     NFELNEVSDYITVLINKLISHYKEDYLVNFIDINENKQDVFKSRKVGLLEGELGVYLT
                     LMTLKNTKILNEKNWTNAFLIS"
     gene            3468..3986
                     /locus_tag="O2701_002344"
     CDS             3468..3986
                     /locus_tag="O2701_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HCZ4294218.1"
                     /translation="MGENVLICLCGSVNSINISHYIIELKSKFDEVNVIASTNGRKFI
                     NGEILKQFCDNYYDEFEDPFLNHVDIANKHDKIIILPATSNTINKIANGICDNLLLTI
                     CHTAFEKLSIFPNMNLRMWENPVTQNNIRLLKDYGVSIYPANISESYELASKTFKKNV
                     VAPEPYKVLEFI"
     gene            3996..5369
                     /locus_tag="O2701_002345"
     CDS             3996..5369
                     /locus_tag="O2701_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HCZ4294219.1"
                     /translation="MKIIKRAIISLIILSLLISITMSNASASEELYYSVEYKNTATFN
                     KLVKKKSLNVVYNIPELHVAQIKMTKMHANALANYKNDIKYINATCSTCITSEKTIDR
                     TSNESLFSRQWDMNKITNNGASYDDLPKHANTKIAIIDTGVMKNHDDLKNNFSTDSKN
                     LVPLNGFRGTEPEETGDVHDVNDRKGHGTMVSGQTSANGKLIGVAPNNKFTMYRVFGS
                     KKTELLWVSKAIVQAANDGNQVINISVGSYIILDKNDHQTFRKDEKVEYDALQKAINY
                     AKKKKSIVVAAAGNDGIDVNDKQKLKLQREYQGNGEVKDVPASMDNVVTVGSTDQKSN
                     LSEFSNFGMNYTDIAAPGGSFAYLNQFGVDKWMNEGYMHKENILTTANNGRYIYQAGT
                     SLATPKVSGALALIIDKYHLEKHPDKAIELLYQHGTSKNNKPFSRYGHGELDVYKALN
                     VANQKAS"
     gene            5392..6084
                     /locus_tag="O2701_002346"
     CDS             5392..6084
                     /locus_tag="O2701_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding subunit"
                     /protein_id="HCZ4294220.1"
                     /translation="MAKLVTENISKRFKNQDVLKHINITLENNEVYGLLGINGAGKTT
                     LMKIICGILQQDSGEIKLDNRPMTRNDLHKVGSLIETPATYNHLSAQDNLKIVCLNES
                     VDFSEINSVLSLVNLNVDKKKKVKDFSLGMKQRLGIAMALIKKPEILVLDEPSNGLDP
                     YGIQELRELLKLLTEQGTSIIISSHILSEIQVLADHIGIIHEGELKYQQRNNKDENLE
                     EIFFKITKGDYK"
     gene            6081..6842
                     /locus_tag="O2701_002347"
     CDS             6081..6842
                     /locus_tag="O2701_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HCZ4294221.1"
                     /translation="MIHLKIEGIKFKNSFSMYVLLISPLVFLCFAIFTVLFAKSNTGT
                     ANSVSPYITLLFNIWPIAFIPIVLCMACNSLFKIEMRNKSFNYYLSNNWSITKEIRAK
                     IFILSIAFLVHCFLVFIIAYIGDLIINPHPVNAMLLLVTILLMYVVSLPLIPLNFLLT
                     RYFGVFVSILINLVLSVICVLFLTLKSLFWVLPWGIMQRIPLITLGILPNGLVVNHNS
                     KYFNDLNALYISIIVSIIIFAIVTFLNNKKSWRLK"
     gene            6839..7537
                     /locus_tag="O2701_002348"
     CDS             6839..7537
                     /locus_tag="O2701_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsaG protein"
                     /protein_id="HCZ4294222.1"
                     /translation="MIINELKSCKLKFSKQALTFVPIIVTILFILFINWYLNVNLWNG
                     RQISLFTASFNAITSLLISINVYQVINFEENIGHFNHILGKANRLNWLNASMIFTYTI
                     TAICILLASINLLWHSHDMKITLMFIGVSLFFNVIILLLLFIFSIFIKDVMAIVVGVL
                     MFIFNVYFGLEVLGDHSWFYLPITYATRYVYMFIEGSIPVTLTLAIYIIITLLLAVLL
                     FKGFNKWSGRTIKD"
     gene            complement(8011..8577)
                     /locus_tag="O2701_002349"
     CDS             complement(8011..8577)
                     /locus_tag="O2701_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4888 domain-containing protein"
                     /protein_id="HCZ4294223.1"
                     /translation="MNTKFLGKTLVASALVLTTLGTGLHSSYLGLDTNKVVKTAKAEE
                     KMTDGQLWKKVKDSLHDSDIILSNEYETINVTYLLSNGYSSSVSAPGNDDGGHLTQSI
                     DFKGLKQIDLTKENVYDDFNKKLDAKNTWNSLTEKLKGLGLLQNGQKVSIYSSDSSSP
                     VSGKVGEGVTSGGENTLTKRFINKITID"
BASE COUNT         3136 a         1088 c         1448 g         2920 t
ORIGIN      
        1 aaaaattaga gaaatccaga aattgatatt agcttatgaa aaaactgaaa ttggatttgg
       61 cgaggaatta tataaagaca ttattcatca tatgaaggca ctattcaaat gtaaaaacta
      121 tcttcaaatt gataccaaaa ttgacatgat taacaattat ttacatcaag atatagcaac
      181 aaatatatct gaagcagcgt acttattgtg gcttttatca agaaataata ttggattcac
      241 agatttgaaa gttcttcaca accgttttat tgaaaagtat ggatttgaac aactcgtgaa
      301 tattaaagat ttgatttcag atataacagg tttcgggaca acaatttttc aagaagaaga
      361 aaccgatgga aataatattg taatgcttaa gcaaaaattt ttacatgcct tgagaaataa
      421 cgatgagata gtcataaatg aaaaagatgt agagtcattg attaacgata atgaaatcaa
      481 tcattatcat gcgccaatga gtgcagatgt ttatgctgaa ctttatttag gacgttttta
      541 caatcagtac aatgagctca ttgttattag tcctttaacg gcgtctttta atgctggcgc
      601 tacatttggg cgatttcatc atttaattga tactgaaact ttagcaaaat tagaacatga
      661 aaaaggacat tattatcaga agatgatatg tgatgacaat gtagaaatga tttctataaa
      721 taacataccg aaatatccga gaaatcataa tgtattaact aatcatgact catacgaata
      781 ttcattgaat ttaggaagta gtaatagtta ttcaaagtat gagcttacct tagatgatat
      841 ttatgttggt gctaccttta acaaattata tttatattct agccaactaa ataaaagggt
      901 actatttgaa tcaaacaata tgtataactt tttaaaagaa tgtaatttat atcgattgtt
      961 acgagaaatt tcaatggaat ctgtgaaatg cattgaacct atgaatgatg tgagtattga
     1021 ttcatttagt tattcaccac gaatcagata taaaaatgtc attcttaaac cagcatattg
     1081 gaaaattaac gagatggtat tacctttacc taaaaatgaa gaatgggatc aacaattttt
     1141 gaaatatcaa gaacaattca atatacctaa tatcgtgaat ttagtttatg gagataacaa
     1201 gcttctacta aatttatcat tagctaatca tagatattta ttgatgaaag aatataagaa
     1261 gcataaacgt gtacgtttag tagaatcatt tttacctcaa tcaaagaatg atcatgtgta
     1321 tgaaattgtt acaccaattt ataaaaaatc atcttatcga ggacccgaaa tcgaaattcc
     1381 aaaatataaa aatacagata ttgaatatga caaagactgg tttgcattac atattcatat
     1441 cgataagcca tcgcaagata catttatcat cgacaatttg tatccatttg taaaacatct
     1501 taaagataag ggagatatag atcaatattt tttaatgcgt tatatcaaac aaggtgacat
     1561 tttaaagttg aggttattca ggaatgacga aaattacgca gaaatataca gcatcttgaa
     1621 gaattggtta ccacatgttc gacaaacgac agaagtttca gattatgaat ttgtatcata
     1681 cgagccagaa ttttttagat atggtggaaa gaacacgatt aatgaaatag aagccttctt
     1741 tgaatatgac acgaatttag ctgtcaatat tattgaaaat gattttaagt ttgatcgacc
     1801 atatattgtt gctatttcaa taatgtattt atttgagatg ttttcaattt ctaatgaaga
     1861 gcgtatggaa attgttaata actatgtacc tacatcattt aaaagtaaag atattagacc
     1921 ttttaaaaat gaacttgtaa ctatatgtaa tcctgctaat aattttgaat atatggctaa
     1981 acactatagt ggtatttatc gaattttaaa agatggcaac caaattttga gcaaattaaa
     2041 tgaagggctt aaaaaaactt taactactaa aaggtcaagg attattggca gcttaattca
     2101 tatgcgctgc aatcgaatat ttggcattga taaggatcaa gaaacatttg ttttatctat
     2161 tgtaaaagaa attgttaaaa ctcaaaagca ttggtgtggt gacaaaaatg attgatatga
     2221 atctaatatt gaagaagaag tttcaagaga tttcagaagt agatgatttc atttcaaaag
     2281 catctactga aaccgattat tttgaacctt caactttatc acatggtata ccaggtatca
     2341 ttttgttttt agatgcttat caaaaagttt tcgatattaa tactgagcaa attgtgcata
     2401 agtacatcat gaaattagca ccatatttac agagtggtca atataacaat tctttgtttg
     2461 gtggactttc aggtattgca ttttcaatgg atttagcatc gcaaaatggc aaaaattatc
     2521 aaaatatttt aaataatatt gatggtcaca ttgtaaatga aatagaaaat aaatttgatc
     2581 aaatattaca agaaccatta aatccattaa attatgatac tgtcagtgga ttagctggga
     2641 tagggagata tttgctaaat agagtagatg agaatgaatt taatgttaaa gcattaaaaa
     2701 gcatattagt atactttaaa gatattcaat attctaaaaa tagctgggta gtcccacaag
     2761 aaagtcaatt tttagagtct gataaaaatt attttactga aggtaatatc aatcttggcc
     2821 ttgcacatgg agtgctagga ccgatgtctt tatttgcatt atgcgtgatt aaaggaatta
     2881 cgattgaaaa tcatcagcac atattaaaag acatgtacaa atttatcatg gacgaaaaat
     2941 tttgtaacca cgaaagatgg ttgcagcgtt acgatttaat ttctgaacgt aatcatttca
     3001 attttattcg gaatggttgg tgttatggca atacgggtgt aatgacgacg ttgtttttaa
     3061 tcggccaagc attacaagat gatgaaataa ttaaaatgtc taaaaaagtg atgctacaag
     3121 tagtaaatga taaagatgaa aatttaataa gtccaactat ttgtcatgga ttgtcatcac
     3181 aaatattaat gttaacaatt atgaatttga attttgaatt aaatgaagtg tctgattata
     3241 tcactgtatt aataaataaa ctgatttctc attataagga agattatctg gtgaatttta
     3301 tagacattaa tgaaaataag caagatgtat ttaaaagtag gaaagttggc cttttagaag
     3361 gtgaattagg ggtctatcta acattgatga cactgaaaaa tacaaaaatt ctcaatgaaa
     3421 aaaattggac caatgcgttt ttaattagct gaatggaggc gtatatcatg ggagaaaatg
     3481 tattgatttg tttgtgtggc tcagttaata gtatcaatat tagccactat attattgaat
     3541 taaaatcaaa atttgatgaa gtaaatgtaa tcgcgtcaac aaacggtaga aagtttatta
     3601 atggtgaaat attaaagcaa ttttgcgata actattatga cgaatttgaa gacccttttt
     3661 taaatcatgt agatatagca aataaacatg acaagattat tattttacct gcgacttcta
     3721 atacgattaa taaaattgca aatggtatat gtgacaattt attattaact atttgtcata
     3781 cagcttttga aaaactttct atatttccaa atatgaattt acgaatgtgg gaaaatccag
     3841 ttactcaaaa taatattcga ttattaaaag attatggtgt atcaatatat ccagcaaata
     3901 tttcagaaag ttatgaatta gcgtcaaaaa catttaaaaa gaatgttgtc gcacctgaac
     3961 catataaagt tctggaattc atttgagatt agaatatgaa aatcataaag cgcgctatta
     4021 ttagtttaat aatattgagt cttttaatat ctattacaat gagtaatgca tcagcgtcag
     4081 aagaactata ttacagtgtt gaatacaaaa atacagcaac atttaacaag ttagttaaaa
     4141 agaagtcctt aaacgttgtc tataatatac cggaattaca tgtggcacag attaaaatga
     4201 cgaaaatgca tgctaatgct ttagcaaact ataaaaatga tattaaatat atcaatgcca
     4261 catgttcaac ttgtattact agcgagaaaa caatagacag aacatcaaat gagtcattat
     4321 tttcaagaca atgggatatg aataaaataa ccaataatgg tgcatcgtat gatgatttgc
     4381 caaaacatgc taacaccaaa atagcaatca tagatacagg tgtgatgaaa aaccatgacg
     4441 atttgaaaaa taatttctcg actgattcta aaaatttagt acctttaaac ggttttagag
     4501 gtactgaacc ggaggaaaca ggtgatgttc acgatgtcaa tgataggaaa ggacatggca
     4561 cgatggtgtc gggtcaaacg agtgctaatg gtaagttaat aggtgttgca ccgaataaca
     4621 aatttacaat gtatcgcgtg tttggtagta aaaaaacaga actgctttgg gtatcaaaag
     4681 cgattgttca agctgcaaat gatggaaatc aagtcattaa tattagtgtt ggtagttata
     4741 ttattttgga caaaaatgac catcaaacat ttagaaaaga tgaaaaagta gaatacgatg
     4801 cgttacagaa agcaatcaat tacgccaaga agaaaaaatc tatcgttgtt gctgcagctg
     4861 gtaatgatgg tattgatgtc aatgacaaac agaaactaaa attacagcgt gaatatcaag
     4921 gtaatggcga agtgaaagat gttcctgcat ctatggacaa tgtcgttaca gtaggatcta
     4981 cagatcaaaa gagtaatcta tctgagtttt ccaattttgg tatgaattat acagatattg
     5041 ctgcgcccgg aggatcattt gcttatttaa atcaattcgg tgtggataaa tggatgaatg
     5101 aagggtatat gcataaggag aacattttaa ctactgccaa taacggaaga tatatttatc
     5161 aagctggaac ttcattagcc acacctaaag tttcgggagc actagcttta atcattgata
     5221 aatatcatct tgaaaaacat ccagataaag cgattgaatt gttatatcag catgggacat
     5281 ctaagaataa taaaccattt agtagatatg ggcatggtga gcttgatgtg tataaagcat
     5341 taaatgtagc aaatcaaaaa gcaagttaat aaatcaaagg agtttttgat tatggcaaaa
     5401 ttagttactg aaaacatttc gaagcggttt aaaaatcaag atgtattaaa gcatattaat
     5461 atcactttag aaaataacga agtttatgga ttacttggta ttaatggagc cggtaaaacg
     5521 acacttatga aaattatatg tggcatactt caacaagatt caggggaaat taaattagat
     5581 aatagaccaa tgacacgaaa tgatttgcac aaagttggtt cgcttattga aacacctgcg
     5641 acatataatc atttaagtgc acaagataat ttgaaaattg tgtgtttaaa tgaaagcgtt
     5701 gatttcagcg aaattaatag tgttttaagc ttagtcaatt taaatgtcga taaaaagaaa
     5761 aaggttaagg acttttcttt aggtatgaaa caaagacttg gaattgcaat ggcattaatt
     5821 aaaaagccag aaattttagt attagacgaa ccatctaatg gtttagaccc atatggaatc
     5881 caagaactta gagaacttct aaaattatta acagaacaag gtactagtat tattatttca
     5941 agtcacattt tatctgaaat ccaagtttta gcagatcata tcggtattat tcatgagggt
     6001 gagctaaaat atcagcaaag aaataacaaa gatgaaaact tagaagagat attcttcaaa
     6061 ataacgaaag gtgattacaa atgatacatt taaagataga aggtatcaaa tttaaaaatt
     6121 ctttcagtat gtatgtttta ttaataagtc cgctggtatt tctttgtttt gctattttca
     6181 cagtcttatt cgccaaaagt aatacgggaa cagcgaatag tgtgtcacca tatataactt
     6241 tactatttaa tatttggcca attgctttca tcccgattgt attatgtatg gcttgtaatt
     6301 cgttatttaa aattgaaatg agaaataaat catttaatta ttacttaagt aataattggt
     6361 cgattacaaa agaaataaga gcaaagattt tcattttatc aatagcattt ttggtacatt
     6421 gctttttagt atttattatt gcttatatag gtgacctaat tattaatcca catccggtta
     6481 atgctatgtt gttattggtg acaatattgt tgatgtatgt agtatctcta ccattgatac
     6541 cgctcaactt tttattaact cgatactttg gtgtgttcgt atcaatatta ataaacttag
     6601 tattatcagt catttgtgtc ttgtttttaa cattgaagag tttattttgg gtgttgccgt
     6661 gggggataat gcagagaatc ccgcttatta cgcttggtat actacctaat ggtttagttg
     6721 taaaccataa ttcaaaatac tttaatgatc tcaatgcctt atatatttcg attattgtta
     6781 gcatcattat tttcgcgata gtaacatttt taaataataa gaaaagttgg cgattaaaat
     6841 gataattaac gaattaaagt catgtaagtt gaaattttct aagcaagcgc tcacatttgt
     6901 acccattatt gtaaccatat tgtttatatt atttataaat tggtatttaa acgtaaattt
     6961 atggaatggt cgacaaataa gtttgtttac agcgagtttt aatgcaatta catcgctatt
     7021 aatttctata aacgtctatc aagttatcaa ttttgaagaa aatattggtc actttaatca
     7081 tattttagga aaagctaata ggctaaattg gttaaatgca tcaatgattt ttacttatac
     7141 tattacagcc atatgtattc tattagcatc aattaattta ttgtggcatt cacatgatat
     7201 gaaaataaca cttatgttta taggcgtatc attgtttttc aatgtaatta tattactgct
     7261 actttttatt tttagtattt tcattaaaga tgtaatggct attgttgtcg gagttttaat
     7321 gtttattttt aacgtttatt ttggattaga agtgcttgga gatcattcgt ggttctattt
     7381 accaatcaca tatgctacac gttacgttta tatgtttatc gaagggagta taccagttac
     7441 attaacattg gcaatctata ttattatcac gttgctatta gcagttttac tctttaaagg
     7501 atttaataaa tggagcggta gaacaattaa agattaaaga ttggggctct aacggtaaaa
     7561 gaaatttagt tgcgttgaaa agggaggaaa gagaacagtt aaatatgaat acaaaataat
     7621 tttgttttcg gacagcaggg gtattagacg cgattgacaa tgtctgttta attaaacgta
     7681 atgtttattt aagcgatgaa tattaggtga aaagtttttg aatttgaatg taattgaggt
     7741 ttattgatta gacattttat tgaattgcgt gttattatat aaatgtaaaa ataagacgac
     7801 atgcgcgaac atgtcgtcta agcaagccaa acgctggctt ctaaaatatt tttttaacca
     7861 ttccaacttg ccaaagttaa tgcggaatgg tttttttatt ttccgctaat tgaaataaaa
     7921 atgacgtttt aatatattat gggctaggtg gtttgtaaga aagggttagt tattaatgtt
     7981 ttatgaatta aggaaatttg agtttaaggt ttaatcaatt gtgattttgt tgatgaagcg
     8041 tttagttaga gtattttcgc caccactagt tactccttct cccactttac ccgagactgg
     8101 agaagagcta tctgaagaat aaatagatac tttttggcca ttttgtagta aaccaagacc
     8161 ttttaacttc tcggttagag aattccatgt atttttagca tctagctttt tgttaaagtc
     8221 atcgtagaca ttttccttag ttaaatcaat ttgttttaat cctttaaagt ctatagattg
     8281 tgttagatgg cctccatcat catttccagg agcagaaacg ctgctagaat atccattgct
     8341 taataaataa gtaacgttga ttgtttcgta ctcgttactt aaaataatat cagaatcatg
     8401 taatgaatct ttaacttttt tccataattg accatctgtc attttttctt ctgctttagc
     8461 cgttttaaca actttatttg tatctaatcc taagtatgaa gaatgtaagc ctgttcctaa
     8521 tgttgttaat actaaagcac ttgctactaa tgttttacct aaaaattttg tattcatttt
     8581 tattgctcct tt
//
LOCUS       DALAGD010000109         8503 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.110, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000109 DALAGD010000000
VERSION     DALAGD010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8503
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.110"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(40..2040)
                     /locus_tag="O2701_002350"
     CDS             complement(40..2040)
                     /locus_tag="O2701_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4294224.1"
                     /translation="MKLRLLWHIVRRQFITQRLVIIPFILAVSVLFMIEYTLVSIGLN
                     SYIKQKNDFLVPFIIIANFFMALLTFIFIFYANHFMMSQRRKEFSIFMTLGMTKKSMR
                     LIVVMETILQFVIISVVSIAGGYLLGAIFFLFIQKIMGSEVATLRYYPFDSVAMFITL
                     IIIAVLMGMLLIFNLFSINFQRPITYQHRSDSSVISRWLRYVLIVIGSAALYLGYFIA
                     LQQDTTFGAFFKIWIVIGLVIIGTYAFFVGISEIIISILQQVSKVYYHPRYFFVVVGM
                     RVRLKMNAVSLATITLLCTFLIVTLTMTLTTYRDMNHTITKLITNDYDLSFSDNSKSQ
                     IERQQTIENIKRDIQGSVNAKDFKVYETTLFRASLEKNRAYYKLKQASDDIPIDFIGN
                     EGAIFSRQSVMITAFTAQDYNRYHQNKLHLDNQSIGMITNVPIFKKQSKVGLNNEVFK
                     VKQLDAHALNLMMIQDSMVLIVKDNNQLQKVKGFYAHEQKNSTISINFNVFGKTKLTT
                     SQIQKLSKKYDASINSKSEMLMVWDRLTGGLIFVGGVVSIVLVIGIFLMMYYKQVSEA
                     YANQHNYDIMKKLGLDNGRIAKITRNQMTFLFAIPITVALIHTLISSNIVYTLLNMIG
                     INNHHIFLTSYVLAVIIISFLYMAMYKITSYIYAKVIHQQRN"
     gene            complement(2037..2792)
                     /locus_tag="O2701_002351"
     CDS             complement(2037..2792)
                     /locus_tag="O2701_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4294225.1"
                     /translation="MLLEVKHVKKVYGKGLNATTALNQMNLSVGAGEFVAIMGESGSG
                     KSTLLNLIASFDGLTEGDIIVDGAHLNNMKNKSKALYRQQMVGFVFQDFNLLPTMTNK
                     ENIMMPLILAGAKRKDIEQRVHQLAVQLHLEGFLNKYPSEISGGQKQRIAIARALVTK
                     PTILLADEPTGALDSKTSKALMMLFQEIHQLEQTILMVTHSNIDASYAERVIFIKDGR
                     LYHEIYRGEESQLAFQQRITDSLALVNGGSVNI"
     gene            complement(2900..3787)
                     /gene="nsaS"
                     /locus_tag="O2701_002352"
     CDS             complement(2900..3787)
                     /gene="nsaS"
                     /locus_tag="O2701_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system sensor histidine kinase NsaS"
                     /protein_id="HCZ4294226.1"
                     /translation="MTFLKSITQEIAIVIVIFALFGLMFYLYHLPLEAYLLALGVILL
                     LLLIFIGIKYLSFVKTISQQQQIENLENALYQLKNEQIEYKNDVESYFLTWVHQMKTP
                     ITAAQLLLERDEPNVVNRVRQEVIQIDNYTSLALSYLKLLNETSDISVTKISINNIIR
                     PIIMKYSIQFIDQKTKIHYEPCHHEVLTDVRWTSLMIEQLINNALKYARGKDIWIEFD
                     EQSNQLHVKDNGIGISEADLPKIFDKGYSGYNGQRQSNSSGIGLFIVKQISTHTNHPV
                     SVVSKQNEGTTFTIQFPDE"
     gene            complement(3798..4463)
                     /gene="nsaR"
                     /locus_tag="O2701_002353"
     CDS             complement(3798..4463)
                     /gene="nsaR"
                     /locus_tag="O2701_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system response regulator NsaR"
                     /protein_id="HCZ4294227.1"
                     /translation="MKILIVEDDFVIAESLASELKKWNYGVIVVEQFDDILSIFNQNQ
                     PQLVLLDINLPTLNGFHWCQEIRKTSNVPIIFISSRIDNMDQIMAIQMGGDDFIEKPF
                     NLSLTIAKIQALLRRTYDLSVANDSLTVKGCTLILDEAKVVYQEQNIQLSLTELQILK
                     LLFQNEDKYVSRTALIEKCWESENFIDDNTLAVNMTRLRKKLNTIGVNDFIITKKNVG
                     YKV"
     gene            complement(4489..4689)
                     /locus_tag="O2701_002354"
     CDS             complement(4489..4689)
                     /locus_tag="O2701_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294228.1"
                     /translation="MILVMLSPLLIIFFIVLSILEERKRTKKKQLEKEKANTLNQNTN
                     DTESSNQEPSLQQDKEQKDNKG"
     gene            4887..6311
                     /locus_tag="O2701_002355"
     CDS             4887..6311
                     /locus_tag="O2701_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCZ4294229.1"
                     /translation="MLSVKKATKFAVANLIIVSTVFSSVTPAIAQSDKSSKDESFAVG
                     NTKNPKNVIFLVGDGMGPSFNTAYRYYKNDPHAKEMTPTAFDSYLKGTNRTYSNDPKQ
                     NITDSAAGGTAFSSGHKTYNGAIGVDSNKQKVKTVLERAKEKGKSTGLVSTAELTDAT
                     PAAYAAHVTSRDDKNEIAKQFYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKKDG
                     YDLATNSKELSKSDKDKVLGLFADKNMPLAIDASKDEPSLADMQQSALSKLERNKKGF
                     FLMVEGASIDKSAHSNDITGVMSEMEGFEKAFDDAIQYAKKHKDTLVVATADHSTGGL
                     TIGKDKGYEWNPQPIKSMKHSGSYMTEKMAKGEDPEKVINEGYGFEFSQDDMKKVKKE
                     NKKLKRLLKKEKDEKSAEVETQTKALQHAIQKPVNDKSYTGWTSDGHTGEDVNTYAYG
                     PRSESFSGNIENTESAKIIFDIFK"
     gene            6381..6491
                     /locus_tag="O2701_002356"
     CDS             6381..6491
                     /locus_tag="O2701_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294230.1"
                     /translation="MLGAWLTRSWYFLNAKYKPPTNVKITANFFIIQTPF"
     gene            complement(6594..7049)
                     /locus_tag="O2701_002357"
     CDS             complement(6594..7049)
                     /locus_tag="O2701_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCZ4294231.1"
                     /translation="MSTNKNDYEHMLFYFAYKTFITTADEIIEKYGMSRQHHRFLFFI
                     NKLPGITIKSLLEILEISKQGSHATLQKLKEQGLIIEKVLETDRRVKKLYSTDKGDQL
                     IAELNKAQDELLQNIYQQVGSDWYDVMEALAKGRPGFDFIKHLKDEKES"
     gene            complement(7294..8055)
                     /locus_tag="O2701_002358"
     CDS             complement(7294..8055)
                     /locus_tag="O2701_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCZ4294232.1"
                     /translation="MAYISLNYHSPTIGMHQNLTVILPEDQSFFNSDTTVKPLKTLML
                     LHGLSSDETTYMRYTSIERYANEHKLAVIMPNVDHSAYANMAYGHSYYDYILEVYDYV
                     HQIFPLSKKRDDNFIAGHSMGGYGTIKFALTQGDKFAKAVPLSAVFEAQNLMDLEWND
                     FSKEAIIGNLSSVKGTEHDPYYLLDKAVAEDKQIPKLLIMCGKQDFLYQDNLDFIDYL
                     SRINVPYQFEDGPGDHDYAYWDQAIKRAITWMVND"
     gene            complement(8171..>8503)
                     /locus_tag="O2701_002359"
     CDS             complement(8171..>8503)
                     /locus_tag="O2701_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCZ4294233.1"
                     /translation="SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSRVTPPNN
                     EQKAPSNPKGEVNHSNKVSKQHKTDALPETGDKSENTNATLFGAMMALLGSLLLFRKR
                     KQDHKEKA"
BASE COUNT         2831 a         1469 c         1264 g         2939 t
ORIGIN      
        1 tgaaaataca tgattgattg tgatttcact gaaacatggt taattgcgtt gttgatgaat
       61 aactttagca taaatatagg aagttatttt gtacatcgcc atatatagaa acgaaattat
      121 aatgacagct agtacgtaac ttgttaaaaa tatatgatgg ttattaatac ctatcatatt
      181 tagtaacgta tatacaatgt tactagaaat taatgtgtga atcagtgcta ctgttattgg
      241 tattgcgaac aagaaagtca tttgatttcg tgttatcttt gctattcttc cattatctaa
      301 accaagtttt ttcataatgt cataattgtg ctgatttgca taggcttctg agacttgttt
      361 ataatacatc attagaaata ttccaatgac aagtacaatt gatactacgc cacctacaaa
      421 tattagtcca cctgtcaaac gatcccaaac cattagcatt tctgatttag aatttattga
      481 cgcatcgtat tttttcgata gcttttgtat ttgtgatgtt gttaatttcg tctttccaaa
      541 tacattaaag tttatcgata ttgtcgaatt cttttgttca tgtgcataaa atccttttac
      601 tttttgtaat tgattgttat ctttaacaat taaaaccata ctatcttgaa tcatcatcaa
      661 atttaacgca tgtgcgtcaa gttgtttcac tttaaagact tcattattaa gaccgacctt
      721 agactgcttt ttaaaaatag gtacattcgt aatcatacct atactttgat tgtctaagtg
      781 taatttgttt tggtgatatc gattataatc ttgtgcagta aaagctgtaa tcattactga
      841 ttgtcttgaa aatatcgcgc cttcattacc aatgaagtct attggaatat catcactagc
      901 ctgttttaac ttgtaataag cacgattctt ttctaaagaa gctctaaata atgtcgtttc
      961 atatactttg aagtcttttg cattaaccga accctgtata tcacgtttaa tattctcaat
     1021 tgtttgttga cgttctattt gtgacttaga attgtcgcta aatgacaaat cataatcatt
     1081 cgtaatcaat ttcgtaatgg tatgattcat atcacgatag gttgttaatg tcattgtgag
     1141 cgttacaatc aaaaatgtac acagcaaagt gattgttgca agactgactg cattcatttt
     1201 aagacgtaca cgcatcccaa ctaccacaaa aaaataccgt ggatgatagt aaacttttga
     1261 tacctgctgc aatatactaa taattatttc acttatacct acaaaaaatg cataagtacc
     1321 gataataact aatcctatga caatccatat tttaaaaaag gcaccaaacg tcgtatcttg
     1381 ttgtaatgca ataaagtaac ctaaatatag tgctgcgctt cctataacaa ttaaaacgta
     1441 acgcaaccat cgtgatatga cactagaatc ggaacgatgt tgataagtta tcggccgttg
     1501 aaaattaata ctaaacaagt tgaatataag tagcatgccc attaatacag caatgataat
     1561 caaagtaata aacatcgcta cagagtcaaa tggataatac cttaacgtcg caacttcact
     1621 gcccattatt ttctgtataa acaagaaaaa tatcgcacca agtaagtatc cgccggcaat
     1681 actaacgact gaaattatca caaattgtaa gatagtttcc atcactacaa ttaaacgcat
     1741 acttttcttg gtcatgccca atgtcataaa aatgctaaac tcttttcgtc tttgtgacat
     1801 cataaagtga tttgcataga aaataaaaat aaaagttaaa agcgccataa aaaaattagc
     1861 tatgataata aatggtacta ggaagtcatt cttctgtttt atgtagctat ttaacccaat
     1921 tgacacaagc gtatattcaa tcatgaatag tacacttacc gctaaaatga atggaatgat
     1981 tacaagtcgc tgcgtaataa attgacgtcg cactatgtgc cataacaatc ttaacttcat
     2041 atattgacac ttcctccatt cacaagtgct aagctatctg ttattcgttg ttgaaaagct
     2101 aattgacttt cttcaccacg atatatttca tgatatagac gcccatcttt aataaaaatg
     2161 actcgctctg cataagacgc atcgatattt gaatgagtta ccattaaaat tgtctgttcc
     2221 aattgatgaa tctcttgaaa taacatcatc aatgcctttg atgtttttga atcaagtgca
     2281 cctgtaggtt catcggctag taaaatcgtc ggcttagtaa ctaatgcacg tgcaatggca
     2341 atgcgttgct tctgaccccc agagatttca gaaggatact tgtttaagaa tccctctaaa
     2401 tgtaattgta ctgccaactg atgtaccctt tgttctatat cttttcgttt agcaccagct
     2461 aaaattaatg gcatcattat attttcttta ttcgtcattg ttggtaaaag attaaaatct
     2521 tgaaaaacaa aacctaccat ttgttgacga tacaatgctt tacttttatt tttcatatta
     2581 tttaaatgtg cgccatccac aataatgtca ccttcagtta gtccatcaaa agaagcaatt
     2641 aaatttagta gtgtagactt ccctgaccca gactcaccca taattgcaac aaattcacca
     2701 gctccaactg ataaattcat ttgattaagt gctgtcgtag cattcaaacc tttaccataa
     2761 acctttttta catgtttcac ttctagcaac atggcatgca cctcatttct ttgttagttc
     2821 tatattaaaa caaaaaaata ctaataacat ttaaattagt aaaaaaatgt tactagtata
     2881 cttacaatat tgaaagtttt tattcatctg gaaattgaat cgtaaatgtt gtaccctcat
     2941 tttgtttaga tacgactgaa acaggatggt ttgtgtgtgt tgaaatttgt tttacgataa
     3001 ataaaccaat cccacttgag ttactttggc gctggccatt ataacctgaa tagcccttat
     3061 caaatatttt aggcaagtcc gcttcactaa taccgatacc attatctttt acgtgtaatt
     3121 gattggattg ctcatcaaat tcaatccata tatctttacc tctcgcatac ttaagtgcat
     3181 tatttattaa ttgttctatc attaaagagg tccatctaac gtcagttaat acttcgtgat
     3241 gacaaggttc ataatggatt tttgtttttt gatcaataaa ctgtattgaa tatttcataa
     3301 taattgggcg aatgatatta ttaatcgaaa ttttagtgac agaaatatca gaagtttcat
     3361 ttaataactt taaataacta agtgctaaac ttgtatagtt atcaatttga ataacctctt
     3421 gacgaacacg attaacaaca ttaggctcat ctctttcaag taacagttgt gctgcagtaa
     3481 tgggtgtttt catttgatgt acccatgtta aaaagtagct ctctacatca tttttatatt
     3541 caatttgttc atttttaagc tgatacaacg cattttctaa gttttcaatt tgttgttgtt
     3601 ggcttatagt ttttacaaaa cttaaatatt taatacctat gaatatgagt aataataata
     3661 aaataacgcc aagtgctagt aaatatgctt ctaatggcaa atgatacagg taaaacatta
     3721 agccaaacaa agcaaaaata actatgacta ttgctatttc ctgagtaata cttttaagaa
     3781 aggtcattgc attcacccta tactttatat ccgacatttt tctttgtaat gataaaatca
     3841 ttaacgccaa tagtatttaa ttttttacgc aggcgcgtca tgttaacagc taatgtgtta
     3901 tcatctatga agttttctga ttcccaacat ttttcaatta aagcagttct acttacatat
     3961 ttatcttcat tttgaaataa taactttaat atttgtaatt cagtcaaaga tagctgtatg
     4021 ttttgttctt gatacacgac ttttgcttca tctaatatta atgtacaacc tttaaccgtc
     4081 aatgaatcat tagctactga caagtcataa gttcgtctca ataatgcttg aattttggca
     4141 atcgttaatg acaagttaaa tggcttttcg ataaaatcat ctccccccat ttgtattgcc
     4201 ataatttggt ccatattatc aatacgggaa ctaataaata taattggcac attagatgtt
     4261 tttcggattt cttgacacca atgaaaacca tttaacgttg gcaaattaat atctagcaat
     4321 acaagctgag gttgattttg gttaaagata gacagtatat catcaaattg ttcaacgaca
     4381 ataacaccgt aattccattt tttaagttca gatgctaaac tctctgctat aacaaaatca
     4441 tcttcaacaa ttaatatttt catctataat cttcttcctt caattgaatt atcctttgtt
     4501 atctttttgt tctttatcct gctgcaatga cggctcttga tttgaacttt ccgtgtcatt
     4561 tgtattttga tttagtgtat ttgctttttc tttctcgagt tgctttttct tcgtacgttt
     4621 acgctcttct aaaatagaca acactataaa gaatatgatt aataatggag ataacattac
     4681 tagtatcatt tttccacctc aaattatatt taattctata ttttcacacg cttctattct
     4741 aacgctttct ataacaaatt gtcatatata ttcatttttt gttattctaa atcactctgt
     4801 ttacatattt ttaactaaaa ctacataatt gctcaaaatt cctcttttac actgaagaca
     4861 tagtcatata ctcaggagga tgtcttatgt tatcagttaa aaaagccaca aaatttgcag
     4921 ttgctaacct tattatcgtg tcaacggttt ttagttctgt cacacctgca attgcgcaat
     4981 ccgataaaag ttctaaagat gaaagtttcg cggttggtaa tactaaaaat ccaaaaaatg
     5041 ttatcttttt agttggcgat ggcatgggtc catcatttaa cacagcttat cgttattata
     5101 aaaatgatcc gcatgctaaa gaaatgaccc ctactgcttt tgattcatat ttaaaaggca
     5161 caaatcgtac atattcaaat gacccaaaac agaatattac tgattctgca gctggcggaa
     5221 cagcctttag ttctggtcat aagacatata atggtgccat cggtgttgac agtaacaagc
     5281 agaaagtaaa gacggtgctt gaacgtgcaa aagaaaaagg taagtcgact ggtcttgttt
     5341 caacagctga attaacagat gcaacaccag cagcatatgc tgcacacgtg acttctagag
     5401 atgataaaaa cgaaatcgct aaacaatttt ataaagataa aatcaacgga aagcataaag
     5461 ttgatgtttt attaggcggc ggcgctaaat atttcggtaa aagtaatggc aatttagata
     5521 aaaaattcaa aaaagatggt tacgaccttg ccacgaacag taaggagtta tcaaaatctg
     5581 ataaagataa agttcttggt ttgtttgcag ataaaaatat gccacttgca atagatgctt
     5641 ctaaagacga accatcatta gctgatatgc aacaaagtgc acttagtaag cttgagcgca
     5701 ataaaaaagg attcttttta atggtcgaag gtgcttctat agataaatca gcgcattcca
     5761 acgatattac aggcgttatg tctgaaatgg aaggttttga aaaagcattc gatgacgcca
     5821 ttcaatatgc taagaaacat aaagatacac ttgtcgttgc aacagcagat cactctactg
     5881 gtggtctaac aattggtaaa gataaaggat acgaatggaa tcctcaaccg attaaatcta
     5941 tgaaacactc tggatcatat atgacagaaa aaatggctaa aggtgaagac cctgaaaaag
     6001 ttattaatga aggttatgga tttgaatttt cacaagatga tatgaaaaag gttaaaaaag
     6061 agaataaaaa gctaaaaaga ctacttaaaa aagaaaaaga cgaaaaatcc gcagaagtag
     6121 aaacgcaaac aaaagcccta caacacgcta ttcagaagcc agtaaatgat aaatcttata
     6181 cagggtggac ttccgatgga catacaggcg aggatgttaa tacctacgct tacggaccta
     6241 gatcagaatc atttagtggt aatatcgaaa atacagaaag cgcaaaaata atatttgata
     6301 tattcaagta aagcataaaa aaattcccga gcactataac aaggtgttcg ggaatttttt
     6361 atggtttaat ttagtactca atgttaggtg cttggttaac acgttcttgg tattttttaa
     6421 atgcgaagta taaaccgcca actaatgtta aaataactgc taattttttc atcattcaaa
     6481 ctcctttcta agatgtgttg aaactgagtt caattaaatt atatgttttt attatacact
     6541 ttttgacata ttttttaaat ttaagaatgc gaagattttt aacatttctg atgctagctt
     6601 tctttttcat ctttcaaatg cttaataaaa tcaaagccag gtcgcccttt agccaatgct
     6661 tccatcacat cataccaatc cgaaccgact tgttgatata tattttgcaa taattcatct
     6721 tgcgccttgt tcaattcagc aatgagttga tcgcctttat ccgtcgaata taattttttg
     6781 acacgtcgat cagtctctaa aactttttca ataatgagac cttgctcttt taatttttga
     6841 agtgttgcat gtgatccttg tttagaaatt tctaatattt ctagtaatga tttaatagta
     6901 ataccaggta atttattgat aaaaaacaaa aaacgatgat gctgacgact cataccatac
     6961 ttctctataa tttcatcagc ggtagtaata aaggttttat atgcaaaata aaacaacata
     7021 tgctcataat cgtttttatt ggtcgacatg atgatgactc ctttcaaagc gttaacctca
     7081 ttataataaa atttataatg taagtcatac aaatgatgat aaaactataa gctaaatcta
     7141 agtttcacga gaaacatttt tgtgtatcag caaattgcgc caatcatatt aatacaaaaa
     7201 taatttaaac acttagaaat gttagtcgtg cgtaaatagt ttgtatctct cactctgtgt
     7261 atttaatgca gccacatatt ttccaagaaa taattaatca ttcaccatcc atgttatagc
     7321 acgcttaatc gcttgatccc aatatgcata atcatgatct cctggtccat cttcaaattg
     7381 ataaggaaca tttatgcgtg ataaataatc gataaaatct aagttgtctt gatataaaaa
     7441 gtcttgttta ccacacataa tgagcaattt tggaatttgt ttatcttcag ctacagcttt
     7501 gtctagcaag taatacggat catgttcagt tcctttaaca cttgaaagat tgccaattat
     7561 ggcctctttt gaaaaatcat tccactctag atccattaaa ttttgcgctt caaacacagc
     7621 agataatggt acagctttgg caaatttatc cccttgtgtt aatgcaaatt taattgtgcc
     7681 atatcctccc atagagtgac ctgctataaa attgtcatca cgctttttgg aaagtggaaa
     7741 tatttgatga acataatcat acacttccaa aatataatca taatagctat gaccatatgc
     7801 catgttagca tatgcgctat gatccacatt gggcataatc acagctaatt tgtgttcatt
     7861 cgcatacctt tctatgcttg tatatctcat atatgtcgtt tcatcacttg ataatccatg
     7921 taacaacatt aaagttttta atggtttaac agttgtatcg ctattaaaga agctttgatc
     7981 ttccggtaaa atgactgtca aattttgatg cataccaatt gttggtgaat gatagtttaa
     8041 tgaaatataa gccatacgtc atgacccctt tctaattcta ctttatcaac attttacgct
     8101 taatcaattc actttaaaat cattttcaac aaaaaaaccg aatacaaatg tattcggcct
     8161 aaaaaagtat ttacgctttt tctttatgat cttgcttgcg ttttctaaac aatagtaatg
     8221 atcctaataa tgccatcatt gcaccaaata aagttgcatt tgtgttttcg ctcttatctc
     8281 ctgtttctgg taaagcatca gttttgtgtt gttttgatac cttattagaa tggtttactt
     8341 cacctttagg atttgatggt gctttctgtt cattatttgg tggtgtaact cttgaatcgg
     8401 agtcactatc tgagtctgag tcgctatctg aatccgagtc gctatccgag tctgagtcgc
     8461 tatctgagtc tgaatcgctg tctgagtctg agtcgctatc cga
//
LOCUS       DALAGD010000110         8492 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.111, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000110 DALAGD010000000
VERSION     DALAGD010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8492
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.111"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..1796
                     /locus_tag="O2701_002360"
     CDS             <1..1796
                     /locus_tag="O2701_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCZ4294234.1"
                     /translation="PVSTTPQSTSGKKDGQSFVDITTTQVSTANENTQNITDKDVKSM
                     EAALTGSLLSLSNNLDTQAKAAQKDSQALRNISYGILASDKPSDFRESLDNVKSGLEY
                     TTQYNQQFIDTLKEIEKNENVDLSKEIDKVKAANNRINESLRLVNQLSNALKNGSSGT
                     AEATKLLDQLSKLDSSLSSFRDYVKKDLNSSLVSISQRIMDELNKGQTALSNVQSKLN
                     TIDQVINSGQAILKNGKTRIDRLQTVLPSIEQQYISAVKNAQANFSKVKSDVAKAANF
                     VRNDLPQLEQRLTNATASVNKNLPTLLNGYDQAVGLLNKNQPQAKKALSDLADFSQNK
                     LPDVEKDLKKANKIFKKLDKDDAVDKLIDTLKNDLKKQAGIIANPINKKTVDVFPVKD
                     YGSGMTPFYTALSVWVGALLMVSLLTVDNKHKSLESVLTTRQVFLGKAGFFIMLGMLQ
                     ALIVSVGDLLILKAGVESPVLFVLITIFCSIIFNSIVYTCVSLLGNPGKAIAIVLLVL
                     QIAGGGGTFPIQTTPQFFQNISPYLPFTYAIDSLRETVGGIVPEILITKLIILTLFGI
                     GFFVVGLILKPVTDPLMKRVSEKVDQSNVTE"
     gene            complement(1908..2846)
                     /gene="manA"
                     /locus_tag="O2701_002361"
     CDS             complement(1908..2846)
                     /gene="manA"
                     /locus_tag="O2701_002361"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HCZ4294235.1"
                     /translation="MALFLQPVFKDRIWGGHALKAFNYDIPNETTGECWAISAHPNGP
                     NTIINGPYKDMTLDQLWSQHRELFDNDSRDSFPLLTKVLDANDKLSVQVHPDDDYALK
                     HEGELGKTECWYILDAAPGAEIIYGVHAHNKQTLIDMIDNHEFDSLFKRIPVKPGDFY
                     YVPAGTVHAIGSGILILETQQSSDTTYRIYDYDRRDQNGQLRDLHLEQSKDVIELGNH
                     EPNTTPITTHIDTHTVTQFVDNQYFAVYKWDIHGDLKFNKPHAYCLVTIIDGTGTLTV
                     NDKHYDIQKGSSFILTTEDEDIHFVGNISAIVSHPG"
     gene            complement(2860..4812)
                     /locus_tag="O2701_002362"
     CDS             complement(2860..4812)
                     /locus_tag="O2701_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HCZ4294236.1"
                     /translation="MKIVAITSCPNGIAHTYMAQEKLEQVAKEMGVDIKVETQGGVGA
                     ENVLTTQDIEEADGVIIAADKQVDLSRFVGKRLINENVREGIHNPRGLIQRIINQDAP
                     IYQSETNYHSKDRGKSKSGIQMVYQHLMNGVSFMVPFIVVGGLLIAIALTLGGETTSK
                     GLVIPDDSFWKSIENIGSLAFKFMVPILAGYIAVSIADKPGLVPGMIGGAIAADGSFY
                     GSDAGAGFLGGIVAGFLAGYIAKWIKDIKVPKAMAPIMPIIIIPIISSVVVGLIFIFL
                     IGAPISNIFEALTTWLKSMQGANIIILAMIIGAMIAFDMGGPVNKVAFLFGSALIAEG
                     NYAVMGMVAVAVCTPPIGLGLATFVQKYKFNHSEREMGKASFTMGLFGITEGAIPFAA
                     QDPLRIIPANIIGAMIASVIAAIGGVGDRVAHGGPIVAVLGGIDHVLWFIFAVIVGSL
                     VTMTTVLLLKRNTPVIAVDAPAQHTQLHDTDITQHDTEVDNVDNTAKHSHLNKPSHVF
                     DQQTMIITDHDMSRNEAIDMLIHKLKICRYVEHTSHLKNAILEREMGSTTAIGMNVAI
                     PHAKSDVVKQPIVAVMKNNHGVKWDSLDGSLPQLIFLIAVPNNSQDTHLKILQRLSKA
                     LMNDETRQSLINANSTTEIYNLLMKI"
     gene            complement(4889..6763)
                     /locus_tag="O2701_002363"
     CDS             complement(4889..6763)
                     /locus_tag="O2701_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCZ4294237.1"
                     /translation="MLDRHLKLLQFFIKNPSKHISSNEIAKHVNVSNRTVRNDIHVIN
                     SNFMDDIIVSIKSKGYQLNTSQYTLETITERYTHIQSYKEKLLLSMAYQLLMHNKSQT
                     LQQLEQDYLLSKTVLNDYFVRIQQWCQKFNIALTIKKKQGIVVDGTDNDITNAIIHLN
                     QLSSGHVHVEDLILNELPDSHQRMISHIIQETLQQHSIETTDIQIQQLLIHLILIIKR
                     KQSLSELDTLNHDSKLISQSCIKQINERLGYDLNEKVINMFSFFIAYHFNKLDIGIER
                     IFIQSYIDRLIELMQQRIHVPFNEDSILQQNLYNHFSKAYLRITQNIYLNNPLVIEIK
                     KLYPFVFNTLFEAIDKLAIDTDIEMSEDEIAFLTIHFQAAIERRTKTQLNVVIACYYG
                     LGVSNFLETKINNLSEELSVINTIKLENITHYHFDNVDLLITTHDIPKQTLNILPKHL
                     TTIKVAPLFSEDDRHKIRHVVKQKQNPVQAHHHMDTVNFLVVNTEQKSRHTVQIFEEA
                     QKILQAHHAIVEGYIESALEREKSSSTYIGNFMAIPHGDPEKVLQSHVLIFRTKDVFP
                     WRQHDVKLVFFLAISHKDKAFTKQMMQLIANLDDDSVNHLCSLDDHSLKQQLFEYLQE
                     "
     gene            complement(7003..7461)
                     /locus_tag="O2701_002364"
     CDS             complement(7003..7461)
                     /locus_tag="O2701_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294238.1"
                     /translation="MKKLLITIVIIVLIGALGFAIYAAINHSTSSSNNQEQENKSTHK
                     KTETEENDQQDDSADKTDKNQNNGNNVTNDNQPSSPSNVPNNHSTVPSNTQPAQPKDS
                     NSNKNNTHGGGSQSSTNNQNNQQQNTPPANKSNNTNTPSKQTPQAQSPKN"
     gene            7719..8246
                     /gene="isaB"
                     /locus_tag="O2701_002365"
     CDS             7719..8246
                     /gene="isaB"
                     /locus_tag="O2701_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunodominant staphylococcal antigen IsaB"
                     /protein_id="HCZ4294239.1"
                     /translation="MNKTSKVCVAATLALGTLIGVTVVENSAPTSKQAQAAITPYYTY
                     NGYIGNNANFILDKNFINAIKYDNVKFNGIKLAKTNTIKKVEKYDQTFKGVSAKGNEA
                     SQLQFVVKNNISLKDIQKAYGKDLKKENGKTKEADSGIFYYQNAKKTLGIWFVVDHNR
                     VVEVTVGHTPYKTSK"
BASE COUNT         2801 a         1340 c         1494 g         2857 t
ORIGIN      
        1 cgcctgtatc gactacacca caaagtacaa gcgggaaaaa agatggtcaa agttttgtag
       61 atataacaac aacacaagtc agcacagcta acgagaacac acaaaacatt acagataaag
      121 atgttaaatc aatggaagcg gcattaacgg gctctttatt atcattatca aataatttag
      181 atacccaagc gaaagccgca caaaaagata gtcaggcatt acgtaatatt tcgtatggga
      241 ttttagcatc ggacaagcct tctgatttta gagagtcttt agataatgtt aagtccggtt
      301 tagaatacac aacgcaatat aatcaacaat ttatcgatac attaaaagag attgagaaga
      361 atgaaaatgt tgatttatca aaagaaattg ataaggtaaa agcagctaat aatcgaatta
      421 atgaatcatt aaggttagtt aatcaattaa gcaatgcatt aaagaatggt agttcaggaa
      481 ctgctgaagc tactaaatta ctagatcaac tttcaaaact agattcatca ttatcatcat
      541 ttagagatta tgttaaaaaa gatcttaaca gctctttagt atcaatatca caacgtatta
      601 tggatgaatt gaacaaaggg caaactgcat tatccaatgt tcagtctaaa ttaaatacaa
      661 ttgatcaagt cattaacagt ggacaagcta ttttaaaaaa tggtaaaaca cgtattgatc
      721 gcttacaaac agtattacca agtattgaac aacaatacat tagtgctgtt aaaaatgctc
      781 aagcaaactt ctcgaaagtg aaaagtgatg tagctaaagc tgctaacttt gtgcgcaatg
      841 acttaccaca gttagaacag cgattaacta atgcgacagc aagtgtgaat aaaaatttac
      901 caacgttatt aaatggttat gatcaagcgg taggattact aaataaaaat cagccacaag
      961 cgaaaaaggc tttatcagat ttagctgatt tttctcaaaa taaattgcct gatgttgaaa
     1021 aagatttgaa aaaagcgaat aaaattttca agaaattaga caaagatgat gcagtcgaca
     1081 aattaatcga cacacttaag aatgatttga aaaagcaagc gggtattatt gcaaatccta
     1141 ttaataagaa gactgttgat gttttcccag ttaaggatta tggttcaggt atgacaccat
     1201 tctatactgc actgtcagta tgggtaggtg cactcttgat ggtaagttta ttaacggttg
     1261 ataataaaca taagagtcta gagtcagtct taacgacaag acaagtgttc ttaggtaagg
     1321 caggattctt tataatgctt ggtatgttgc aagcactcat tgtatcggtt ggagatttgt
     1381 taatcctaaa agcaggagtt gagtcacctg tattatttgt acttataacg attttctgtt
     1441 cgattatttt caactcaatc gtatatacgt gcgtatcatt acttggtaac ccaggtaaag
     1501 ccattgcaat cgtattgctt gtattacaaa ttgcaggtgg tgggggaaca ttcccaattc
     1561 aaactacgcc acaatttttc caaaacattt cgccatactt accatttacg tatgcaattg
     1621 attcattacg tgaaacagta ggcggtattg ttccggaaat cctaattaca aaattaatta
     1681 tattaacgtt atttggtata ggattcttcg ttgtaggttt aattttaaaa cctgtaacag
     1741 atccattgat gaagcgcgta tctgaaaaag ttgaccaaag taacgttaca gaataaaaat
     1801 taaatccaca cattagggtt atagctcctt aatgtgtgga tttttatgtt tttagacaga
     1861 agagatagta atttctgtct tttatgggac ggttgttatc attgctatta tccaggatga
     1921 cttactatag cactaatatt accgacaaag tgaatatcct cgtcttccgt agttaaaata
     1981 aagctagaac ctttttggat gtcatagtgc ttatcgttta ctgttaaagt accagtacca
     2041 tcgataattg taactaagca ataagcatgt ggtttattga attttaaatc tccatgaata
     2101 tcccatttat atactgcaaa atattgatta tctacaaatt gagttacagt gtgtgtgtcg
     2161 atgtgagttg ttataggagt agtatttggt tcatgattgc ctaattcaat cacatcttta
     2221 ctttgctcta agtgcaaatc acgcaattga ccattttgat ctcgtctatc atagtcataa
     2281 atacggtatg tcgtatcgga ggattgttgt gtctctaaaa ttaaaatacc cgaaccaatg
     2341 gcatggacag tgccagcagg aacataataa aagtcaccgg gcttaacagg tatacgtttg
     2401 aaaagactgt caaattcatg attatcaatc atgtctatta acgtctgttt attatgtgca
     2461 tgtacgccat atataatttc agcacctggg gctgcatcta aaatatacca acattctgtt
     2521 ttacctagtt cgccttcgtg ttttaaagcg tagtcatcat ctgggtgaac ttgaacagat
     2581 aatttatcat tggcatctaa tactttagtt agcagaggga aactatctcg tgaatcatta
     2641 tcgaataatt cacgatgttg tgaccaaagt tgatctaggg tcatatcctt gtatggacca
     2701 ttgataattg tattaggacc atttggatgt gcagaaattg cccagcattc accagttgtt
     2761 tcattaggga tatcatagtt aaatgctttt aatgcatgac cgccccaaat tctgtcttta
     2821 aaaacgggtt gtaaaaataa tgccatagtt aaaactcctc tatattttca ttaataagtt
     2881 ataaatttct gtagtactgt ttgcattaat tagtgattgg cgtgtctcat cattcattaa
     2941 cgctttagat aagcgctgaa gtatttttaa atgtgtatcc tgactgttgt ttggtacggc
     3001 aattaagaat atcaattgag gtagactacc atctagactg tcccatttaa caccatgatt
     3061 atttttcata acagctacaa tcggttgttt tacaacatca gactttgcat gtggaatggc
     3121 cacgttcatg ccaatagctg tcgtagaccc catttcacgt tctagtattg cattttttaa
     3181 atgcgatgtg tgctctacat aacggcaaat tttaagttta tgaatcaaca tatcaattgc
     3241 ttcgtttcga gacatgtcgt gatcagtaat tatcatagtt tgttgatcaa aaacatgaga
     3301 aggtttattg agatgtgaat gtttcgcggt gttatctaca ttgtcaacct ctgtatcatg
     3361 ttgtgtaata tctgtatcat gaagttgcgt gtgttgcgct ggtgcatcta ctgctataac
     3421 tggtgtattg cgttttaata atagtacagt agtcattgtg acaagactac ctactatcac
     3481 tgcaaagata aaccataata catgatcaat accacctaat acagccacga ttggacctcc
     3541 atgtgcgact ctatcgccga caccaccaat ggctgcaatg actgatgcaa tcattgcacc
     3601 aatgatgttt gcaggtataa tgcgcaatgg atcttgggct gcgaaaggaa tagcaccttc
     3661 agtaatacca aatagtccca tagtgaagga agccttaccc atttctcttt cggaatgatt
     3721 gaatttatac ttttgaacaa acgttgctaa acctaaaccg attggtggtg tacatacagc
     3781 aactgcgacc atacccataa cggcgtaatt accttcagca ataagtgctg agccaaataa
     3841 aaatgctacc ttgtttactg gaccgcccat atcgaaggca atcatcgcac ctataatcat
     3901 cgcaagtata ataatattag caccttgcat actttttaac caggttgtta atgcctcaaa
     3961 aatattagaa attggtgcac cgattaaaaa tataaatatc aatcctacaa cgaccgatga
     4021 aataatggga ataataatga taggcataat tggtgccatt gcttttggaa ctttaatatc
     4081 tttaatccac tttgcgatat aacctgctaa gaaaccagca acaataccac ctaaaaatcc
     4141 tgcgcctgca tcactgccat aaaaactacc gtcagcagcg atagcgccgc caatcatacc
     4201 aggaacaaga ccgggcttgt cagcgatact aacagcgata tatccagcta gtattggaac
     4261 cataaattta aaggctaaac taccaatgtt ttcaatggat ttccaaaatg aatcatctgg
     4321 gatgactaat ccttttgatg tcgtttcacc gcctagagtc agcgcgatgg cgataaggag
     4381 tccaccaact acgataaaag gaaccataaa cgatacaccg ttcattaaat gttgatacac
     4441 catttgaata ccacttttag acttaccgcg atctttcgaa tgataatttg tttcagattg
     4501 ataaataggc gcatcttgat taatgatacg ttgaattaga cctctcggat tatgaatccc
     4561 ttcgcgaaca ttttcattaa tcaaccgttt accaacaaat cgggacagat caacttgttt
     4621 atcagctgca attatgacac cgtcagcttc ttcgatgtct tgcgtagtta aaacattttc
     4681 agcaccaaca ccgccctgtg tctctacttt aatatccaca cccatttctt ttgctacctg
     4741 ctcaagcttt tcttgagcca tatatgtatg tgcaatgcca tttgggcatg aggtaatagc
     4801 tacaattttc ataaaatcat ctccttttct atattgtaag cgtattctcg atactaaaaa
     4861 aaagaataat taccgttact agtggcaatt attcttgtaa gtattcaaat aactgttgct
     4921 ttaaactatg atcatctaaa ctacataaat ggttcactga atcatcatcc aagttagcaa
     4981 ttaattgcat catttgtttt gtaaaagctt tgtctttatg cgaaatcgct aagaaaaaga
     5041 caagtttgac atcgtgttgt cgccaaggaa aaacatcttt tgtgcgaaaa ataagcacat
     5101 gtgattgtaa aactttttca ggatctccat gaggaatcgc cataaaatta cctatgtatg
     5161 tagaagatga tttctcacgc tctaaagctg attcgatata tccttctaca atcgcatgat
     5221 gtgcttgtaa tattttttga gcttcttcaa aaatttgcac agtatgccgt gatttttgtt
     5281 cagtatttac gacaaggaaa ttgacagtgt ccatatgatg atgtgcttga accggatttt
     5341 gcttttgctt cacaacgtgt ctgattttgt gacgatcatc ttcagaaaat aatggtgcaa
     5401 ccttgatagt cgtcaggtgc ttaggaagta tgtttagcgt ttgtttagga atatcatggg
     5461 tcgttattaa taaatctaca ttgtcaaagt gatagtgtgt tatattttct agtttaatcg
     5521 tatttatcac tgacaactct tcggataagt tatttatttt agtttctaaa aaattcgaca
     5581 cacctagacc ataataacaa gcaatgacta catttaattg tgttttggta cgacgctcga
     5641 tggcagcttg aaaatgaatt gttaaaaatg caatttcatc ttcgctcatc tctatatcag
     5701 tatcaattgc taatttatca atcgcttcaa aaagtgtgtt aaacacaaag ggatagagtt
     5761 ttttaatctc tataactaaa ggattgttta aataaatgtt ttgagtgata cgtaaatatg
     5821 ctttactaaa atgattatat aaattttgtt gtaaaatcga atcttcattg aaaggtacat
     5881 gaatacgttg ctgcatcaat tcgattaagc gatcaatata actttgtata aatatacgtt
     5941 ctatgccaat atcgagttta ttaaaatgat aagcaataaa gaatgaaaac atattgatta
     6001 ctttttcgtt caagtcataa cctaatcttt cgttgatttg cttaatgcaa gattgagata
     6061 tcaattttga atcgtggttt aacgtgtcta gctcacttaa agattgctta cgtttaatga
     6121 ttaaaatgag atgaattaaa agctgttgta tttgaatatc agttgtttca atactatgtt
     6181 gttgaagtgt ctcttgtata atatgcgaaa tcatcctttg gtgtgaatca ggtaattcat
     6241 ttaaaattag gtcttcaaca tgtacatgcc ctgatgataa ttgatttaaa tggatgatgg
     6301 cattagtgat atcattatct gttccatcga ccacaatacc ttgttttttc ttgattgtta
     6361 aagcgatatt aaatttttga caccattgtt gaattcgaac gaaatagtcg ttgagcacag
     6421 ttttagataa tagataatct tgctctagtt gttgcaatgt ctgcgattta ttgtgcatta
     6481 gcagttgata cgccatagat aataatagtt tttctttata tgattgaata tgcgtgtaac
     6541 gttcagttat cgtttctaag gtatactgag atgtatttaa ttgatatcct ttagatttaa
     6601 tacttacgat gatatcatcc ataaaattgc tattgataac atggatatca ttgcgaactg
     6661 tgcggtttga aacattgaca tgtttagcaa tttcattaga actaatgtgc tttgatggat
     6721 ttttaataaa aaactggagt agttttaagt gtctatcaag catttaacat gtacctcctt
     6781 tctaaatttt tcgtgtaagc gtttttaagg gtgtattaat attattaaac atgagagctt
     6841 atacatacgt caatgacatt aaagcgaact tttatatgat tttaacagag tgcgaattat
     6901 gcaaataaag aacagcagta agatatttca aatagaaaaa tatctcactg ctgtttttct
     6961 gaatttatgc atctggtact tgtggacgta tcaggcaaat gattaatttt taggtgattg
     7021 tgcttgaggt gtttgtttag aaggcgtatt ggtattattt gatttattag caggtggtgt
     7081 gttctgttgt tgattatttt ggttgtttgt tgacgattga ctgccaccac cgtgagtatt
     7141 atttttattt gaatttgagt cttttggctg agctggttgc gtattcgatg gtactgttga
     7201 atggttgtta ggaacatttg acggtgaaga tggttgattg tcattagtaa cgttattgcc
     7261 attattttga tttttatctg ttttgtctgc actatcatct tgttgatcat tttcttcggt
     7321 ttctgtcttt ttatgcgtag atttattttc ttgttcttgg ttattacttg atgatgtaga
     7381 atgatttatt gcagcataaa ttgcgaaccc aagtgctccg attaatacga taattacgat
     7441 ggtaataaga agttttttca aatataacgc ctccatgtta atgaaaagta gttgatacgt
     7501 gaagctaaat aatttatttc aatattgcca taatcatttt aaaaaataaa tattagaaaa
     7561 gtaggcgata ttaatatttt cattgttttt tgtgtacttc aattagtata acaaaactgt
     7621 tatgtattta taaatataat cattagtatt tttatggctg aaaaagttat aataaaagtg
     7681 taaggaataa aatatttgta tggaaaagag agataattat gaataaaacc agtaaagttt
     7741 gtgtagcagc aacattagca ttgggcacac tgattggagt aacagttgtt gaaaattcgg
     7801 cgccaacaag taaacaggca caggcagcaa taaccccata ttatacttat aatggttata
     7861 ttggtaataa tgctaatttt attctggata agaattttat taacgcgatt aagtatgata
     7921 atgtgaaatt caatggtatt aaattagcta aaacgaatac gataaaaaaa gtagagaaat
     7981 atgatcaaac ttttaaaggt gtttctgcaa aaggaaacga agcaagtcaa ttgcaatttg
     8041 tagttaaaaa taatatttca ttaaaagata tccaaaaagc ttatggcaag gacttgaaaa
     8101 aagaaaatgg taaaacaaag gaagctgata gcggtatttt ttactatcaa aatgctaaaa
     8161 agacattagg catctggttt gtcgttgatc ataatagagt tgtcgaagta acagttggac
     8221 atacaccata caaaacaagt aaataaaata atggcatatt aaggctagag tgtgaggagt
     8281 gataccgcat tctagtcttt tttattaaat aataacgatt attgcgtctt acatagttgt
     8341 ttgaaattga taaaaatgtt tatttgatta tgattattta aatagttatc tcacatattt
     8401 caagcattga ttaagtatat agagcactat tttgtatttg ttaatatttt cacaaaaata
     8461 aagccttgat aaattttaaa atataattaa gc
//
LOCUS       DALAGD010000111         8433 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.112, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000111 DALAGD010000000
VERSION     DALAGD010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8433
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.112"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            105..260
                     /locus_tag="O2701_002366"
     CDS             105..260
                     /locus_tag="O2701_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294240.1"
                     /translation="MMVKKLLKAIKQNFLYEQSNITFKMIVFGYKRLSKMLFQFEINH
                     INFLWEG"
     gene            268..789
                     /locus_tag="O2701_002367"
                     /pseudo
     CDS             268..789
                     /locus_tag="O2701_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499366.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            928..1458
                     /locus_tag="O2701_002368"
     CDS             928..1458
                     /locus_tag="O2701_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCZ4294241.1"
                     /translation="MQIRQIHQHDFAQVDQLIRTAFENSEHGYGNESELVDQIRLSDT
                     YDNTLELVAVLQNEVVGHGLLSEVYLDNEAQREIGLVLAPVSVDIHHQNKGIGKRLIQ
                     ALEREAILKGYNFISVLGWPTYYANLGYQRASMYDIYPPYDGIPDEAFLIKELKVNSL
                     AGKTGTINYTSAFEKI"
     gene            1721..4294
                     /gene="clfA"
                     /locus_tag="O2701_002369"
     CDS             1721..4294
                     /gene="clfA"
                     /locus_tag="O2701_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfA"
                     /protein_id="HCZ4294242.1"
                     /translation="MNMKKKEKHAIRKKSIGVASVLVGTLIGFGLLSSKEADASENSV
                     TQSDSASNESKSNDSSSVSAAPKTDDTNVSDTKTSSNTNNGETSVAQNPAQQETTQSS
                     STNATTEETPVTGEATTTTTNQANTPATTQSSNTNAEELVNQTSNETTSNDTNTVSSV
                     NSPQNSTNAENVSTTQDTSTEATPSNNESAPQSTDASNKDVVNQAVNTSAPRMRAFSL
                     AAVAADAPAAGTDITNQLTNVTVGIDSGTTVYPHQAGYVKLNYGFSVPNSAVKGDTFK
                     ITVPKELNLNGVTSTAKVPPIMAGDQVLANGVIDSDGNVIYTFTDYVNTKDDVKATLT
                     MPAYIDPENVKKTGNVTLATGIGSTTANKTVLVDYEKYGKFYNLSIKGTIDQIDKTNN
                     TYRQTIYVNPSGDNVIAPVLTGNLKPNTDSNALIDQQNTSIKVYKVDNAADLSESYFV
                     NPENFEDVTNSVNITFPNPNQYKVEFNTPDDQITTPYIVVVNGHIDPNSKGDLALRST
                     LYGYNSNIIWRSMSWDNEVAFNNGSGSGDGIDKPVVPEQPDEPGEIEPIPEDSDSDPG
                     SDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASDSDSD
                     NDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSDSESDSNSDSESGSNNNVVPP
                     NSPKNGTNASNKNEAKDSKEPLPDTGSEDEANTSLIWGLLASIGSLLLFRRKKENKDK
                     K"
     gene            4515..6041
                     /gene="vwb"
                     /locus_tag="O2701_002370"
     CDS             4515..6041
                     /gene="vwb"
                     /locus_tag="O2701_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor binding protein Vwb"
                     /protein_id="HCZ4294243.1"
                     /translation="MKNKLLVLSLGALCVSQIWESNRASAVVSGEKNPYVSESLKLTN
                     NKNKSRTVEEYKKSLDDLIWSFPNLDNERFDNPEYKEAMKKYQQRFMAEDEALKKFFS
                     EEKKIKNGNTDNLDYLGLSHERYESVFNTLKKQSEEFLKEIEDIKKDNPELKDFNEEE
                     QLKCDLELNKLENQILMLGKTFYQNYRDDVESLYSKLDLIMGYKDEERANKKAVNKRM
                     LENKKEDLETIIDEFFSDIDKTRPNNIPVLEDEKQEEKNHKNMAQLKSDTEAAKSDES
                     KRSKRSKRSLNTQNHKPASQEVSEQQKAEYDKRAEERKARFLDNQKIKKTPVVSLEYD
                     FEHKQRIDNENDKKLVVSAPTKKPTSPTTYTETTTQVPMPTVERQTQQQIIYNAPKQL
                     AGLNGESHDFTTTHQSPTTSNHTHNNVVEFEETSALPGRKSGSLVGISQIDSSHLTER
                     EKRVIKREHVREAQKLVDNYKDTHSYKDRINAQQKVNTLSEGHQKRFNKQINKVYNGK
                     "
     gene            6392..7414
                     /gene="emp"
                     /locus_tag="O2701_002371"
     CDS             6392..7414
                     /gene="emp"
                     /locus_tag="O2701_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix protein-binding adhesin
                     Emp"
                     /protein_id="HCZ4294244.1"
                     /translation="MKKKLLVLTMSTLFATQIMNSNHAKASVTESVDKKFVVPESGIN
                     KIIPAYDEFKNSPKVNVSNLTDNKNFVASEDKLNKIADSSAASKIVDKNFVVPESKLG
                     NIVPEYKEINNRVNVATNNPASQQVDKHFVAKGPEVNRFITQNKVNHHFITTQTHYKK
                     VITSYKSTHVHKHVNHAKDSINKHFIVKPSESPRYTHPSQSLIIKHHFAVPGYHAHKF
                     VTPGHASIKINHFCVVPQINSFKVIPPYGHNSHRMHVPSFQNNTTATHQNAKVNKAYD
                     YKYFYSYKVVKGVKKYFSFSQSNGYKIGKPSLNIKNVNYQYAVPSYSPTHYVPEFKGS
                     LPAPRV"
     gene            7749..8219
                     /locus_tag="O2701_002372"
     CDS             7749..8219
                     /locus_tag="O2701_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294245.1"
                     /translation="MKRKVLVLTMGVICATQLWHSNHANALVTESGANDTKQFTEIVS
                     EEKVITVEHAQINIFQSNSNSNLMEFNILTMGGKSGAMVGYSEIDSSHFTDRDKRVIR
                     RDHVKEAQSLVENYKDTQSADARMKAKQKVNTLSKPHQNYFNKQIDKVYNGLQR"
BASE COUNT         3277 a         1229 c         1492 g         2435 t
ORIGIN      
        1 gacgtgttat taatttggaa aatactggtc aaagatggga gctcttaaaa gcgttattgt
       61 attttttagt caatacaaat agattgccgt aataataatc gtacttgatg gttaaaaaat
      121 tacttaaggc tataaagcaa aactttttat atgagcagtc gaatataacg tttaaaatga
      181 ttgtttttgg atataaacga ttaagtaaaa tgctttttca gtttgaaatt aatcatataa
      241 atttcttatg ggagggttga tatcttaatg attaacatta tttcagctat aggatctatt
      301 ggaacattta ttatggcttt attttatttt gtatcagttt cagttcaact ttatcaaatg
      361 aaaattagct ttctgccagc tttaggtttt aaccaaattt tattagaaag ggaggaggat
      421 caacttaata taatgaattc ggcaacagaa gagcatcatc ataaagatta tattaaacta
      481 tataatttag gtggcggtgc tgctaaaaaa attgcaatag aggttttatt ggggaaggat
      541 aaagtcattc agaaaaaata cgtgcatatt ttacctagta aagaagggta catgttacca
      601 attaataaaa atgtgtacga agaattagaa agaacgattg agaacaatgg tcatgaagct
      661 gatttgaatg tacgtatgac ttattatcat aatgtaagtc gcaaacaata ggaagttata
      721 ttaaaaggtc aaatcgaccg ttttaatact tataataata aagaaattta tgatttgcag
      781 tttatctaaa aattgattta agagggtagt tgtttattgc gaaaaatatc attcaatttt
      841 aatgaaataa tggcgtcatt actataaaat attactttat gttgtaatgc atttttctat
      901 aagatagaac taaaaggagg ggcaaagatg caaattagac aaatacatca acatgacttt
      961 gctcaagtgg accagttaat tagaacggca tttgaaaata gtgaacatgg ttatggtaat
     1021 gaatcagagc tagtagacca aattcgtcta agtgatacgt atgacaatac cttagaatta
     1081 gtagctgttc ttcaaaatga agttgtaggg cacggtttac taagtgaagt ttatcttgat
     1141 aacgaggcac aacgggaaat tggattagtg ttagcacctg tatctgttga tattcatcat
     1201 caaaataaag gtattgggaa gcgattgatt caagcattag aacgagaagc aatattaaaa
     1261 ggatataatt ttatcagtgt attaggatgg ccgacgtatt atgccaatct aggatatcaa
     1321 cgcgcaagta tgtacgacat ttatccacca tatgatggta taccagacga agcgttttta
     1381 attaaagaat taaaagtgaa cagtttagcg ggaaaaacag gtaccataaa ttacacatct
     1441 gcttttgaaa aaatatgatt tcaagctagg attacattag gtagagttca tattaataat
     1501 aaaaaatgtt tgcaatcaaa tcgtacgttg tcgtttgtaa ttcttaaaat agcaataaat
     1561 aaaatgtttg ttagtaaagt attattgtgg ataataaaat atcgatacaa attaattgct
     1621 ataatgcaat tttagtgtat aattccatta acagagatta aatatatctt taaagggtat
     1681 atagttaata taaaatgact ttttaaaaag agggaataaa atgaatatga agaaaaaaga
     1741 aaaacacgca attcggaaaa aatcgattgg cgtggcttca gtgcttgtag gtacgttaat
     1801 cggttttgga ctactcagca gtaaagaagc agatgcaagt gaaaatagtg ttacgcaatc
     1861 tgatagcgca agtaacgaaa gcaaaagtaa tgattcaagt agcgttagtg ctgcacctaa
     1921 aacagacgac acaaacgtga gtgatactaa aacatcgtca aacactaata atggcgaaac
     1981 gagtgtggcg caaaatccag cacaacagga aacgacacaa tcatcatcaa caaatgcaac
     2041 tacggaagaa acgccggtaa ctggtgaagc tactactacg acaacgaatc aagctaatac
     2101 accggcaaca actcaatcaa gcaatacaaa tgcggaggaa ttagtgaatc aaacaagtaa
     2161 tgaaacgact tctaatgata ctaatacagt atcatctgta aattcacctc aaaattctac
     2221 aaatgcggaa aatgtttcaa caacgcaaga tacttcaact gaagcaacac cttcaaacaa
     2281 tgaatcagct ccacagagta cagatgcaag taataaagat gtagttaatc aagcggttaa
     2341 tacaagtgcg cctagaatga gagcatttag tttagcggca gtagctgcag atgcaccggc
     2401 agctggcaca gatattacga atcagttgac gaatgtgaca gttggtattg actctggtac
     2461 gactgtgtat ccgcaccaag caggttatgt caaactgaat tatggttttt cagtgcctaa
     2521 ttctgctgtt aaaggtgaca cattcaaaat aactgtacct aaagaattaa acttaaatgg
     2581 tgtaacttca actgctaaag tgccaccaat tatggctgga gatcaagtat tggcaaatgg
     2641 tgtaatcgat agtgatggta atgttattta tacatttaca gactatgtaa atactaaaga
     2701 tgatgtaaaa gcaactttga ccatgcccgc ttatattgac cctgaaaatg ttaaaaagac
     2761 aggtaatgtg acattggcta ctggcatagg tagtacaaca gcaaacaaaa cagtattagt
     2821 agattatgaa aaatatggta agttttataa cttatctatt aaaggtacaa ttgaccaaat
     2881 cgataaaaca aataatacgt atcgtcagac aatttatgtc aatccaagtg gagataacgt
     2941 tattgcgccg gttttaacag gtaatttaaa accaaatacg gatagtaatg cattaataga
     3001 tcagcaaaat acaagtatta aagtatataa agtagataat gcagctgatt tatctgaaag
     3061 ttactttgtg aatccagaaa actttgagga tgtcactaat agtgtgaata ttacattccc
     3121 aaatccaaat caatataaag tagagtttaa tacgcctgat gatcaaatta caacaccgta
     3181 tatagtagtt gttaatggtc atattgatcc gaatagcaaa ggtgatttag ctttacgttc
     3241 aactttatat gggtataact cgaatataat ttggcgctct atgtcatggg acaacgaagt
     3301 agcatttaat aacggatcag gttctggtga cggtatcgat aaaccagttg ttcctgaaca
     3361 acctgatgag cctggtgaaa ttgaaccaat tccagaggat tcagattctg acccaggttc
     3421 agattctggc agcgattcta attcagatag cggttcagat tcgggtagtg attctacatc
     3481 agatagtggt tcagattcag cgagtgattc agattcagca agtgattcag actcagcgag
     3541 tgattcagat tcagcaagcg attccgactc agcgagcgat tccgactcag acaatgactc
     3601 ggattcagat agcgattctg actcagacag tgactcagat tccgacagtg actcagattc
     3661 agatagcgat tctgactcag acagtgactc agattcagat agcgattcag attcagatag
     3721 cgattcagat tccgacagtg attccgactc agacagcgat tctgactccg acagtgattc
     3781 cgactcagac agcgattcag attccgacag tgattccgac tcagatagcg attccgactc
     3841 agatagcgac tcagattcag acagcgattc agattcagac agcgattcag attcagatag
     3901 cgattcagat tccgacagtg actcagattc cgacagtgac tcggattcag atagcgattc
     3961 agattccgac agtgactcag attccgacag tgactcagac tcagacagtg attcggattc
     4021 agcgagtgat tcggattcag atagtgattc cgactcagaa agtgattcaa atagcgattc
     4081 cgagtcaggt tctaacaata atgtagttcc gcctaattca cctaaaaatg gtactaatgc
     4141 ttctaataaa aatgaggcta aagatagtaa agaaccatta ccagatacag gttctgaaga
     4201 tgaagcaaat acgtcactaa tttggggatt attagcatca ataggttcat tactactttt
     4261 cagaagaaaa aaagaaaata aagataagaa ataagtaata atgatattaa attaatcata
     4321 tgattcatga agaagccacc ttaaaaggtg gcttttttac ttggattttc caaatatatt
     4381 gtttgaatat aattaataat taattcatca acagttaatt attttaaaaa ggtagatgtt
     4441 atataatttg gcttggcgaa aaaatagggt gtaaggtagg ttgttaatta gggaaaatta
     4501 aggagaaaat acagttgaaa aataaattgc tagttttatc attgggagca ttatgtgtat
     4561 cacaaatttg ggaaagtaat cgtgcgagtg cagtggtttc tggggagaag aatccatatg
     4621 tatctgagtc gttgaaactg actaataata aaaataaatc tagaacagta gaagagtata
     4681 agaaaagctt ggatgattta atatggtcct ttccaaactt agataatgaa agatttgata
     4741 atcctgaata taaagaagct atgaaaaaat atcaacagag atttatggct gaagatgagg
     4801 ctttgaagaa attttttagt gaagagaaaa aaataaaaaa tggaaatact gataatttag
     4861 attatctagg attatctcat gaaagatatg aaagtgtatt taatactttg aaaaaacaaa
     4921 gtgaggagtt cttaaaagaa attgaagata taaaaaaaga taaccctgaa ttgaaagact
     4981 ttaatgaaga ggagcaatta aagtgcgact tagaattaaa caaattagaa aatcagatat
     5041 taatgttagg taaaacattt tatcaaaact atagagatga tgttgaaagt ttatatagta
     5101 agttagattt aattatggga tataaagatg aagaaagagc aaataaaaaa gcagttaaca
     5161 aaaggatgtt agaaaataaa aaagaagact tagaaaccat aattgatgaa ttttttagtg
     5221 atatagataa aacaagacct aataatattc ctgttttaga agatgaaaaa caagaagaga
     5281 aaaatcataa aaatatggct caattaaaat ctgacactga agcagcaaaa agtgatgaat
     5341 caaaaagaag caagagaagt aaaagaagtt taaatactca aaatcacaaa cctgcatctc
     5401 aagaagtttc tgaacaacaa aaagctgaat atgataaaag agcagaagaa agaaaagcga
     5461 gatttttgga taatcaaaaa attaagaaaa cacctgtagt gtcattagaa tatgattttg
     5521 agcataaaca acgtattgac aacgaaaacg acaagaaact tgtggtttct gcaccaacaa
     5581 agaaaccaac atcaccgact acatatactg aaacaacgac acaggtacca atgcctacag
     5641 ttgagcgtca aactcagcaa caaattattt ataatgcacc aaaacaattg gctggattaa
     5701 atggtgaaag tcatgatttc acaacaacgc atcaatcacc aacaacttca aatcacacgc
     5761 ataataatgt tgttgaattt gaagaaacgt ctgctttacc tggtagaaaa tcaggatcac
     5821 tggttggtat aagtcaaatt gattcttctc atctaactga acgtgagaag cgtgtaatta
     5881 agcgtgaaca cgttagagaa gctcaaaagt tagttgataa ttataaagat acacatagtt
     5941 ataaagaccg aataaatgca caacaaaaag taaatacttt aagtgaaggt catcaaaaac
     6001 gttttaataa acaaatcaat aaagtatata atggcaaata attaatgcat ggctgcaaag
     6061 caaataatga gtttgtcgta aaaataacaa cattttaaac tagcaataaa taatatcaaa
     6121 gtcatcattt caatgatgca atctagtata gtccacattc taaacaggtg tggactatta
     6181 cttttttcac tttatattac gaaaaaatta ttatgcttaa ctatcaatat caataattaa
     6241 ttttaagctg aaaaacaata aaaatgttaa gacaacgttt acttcaagtt aattattata
     6301 ctgaaaattc tggtatataa tgctgttagt gaatataaca gggaaattat attggttata
     6361 atattgagtc tatataaagg agaaataaca gatgaaaaag aaattattag ttttaactat
     6421 gagcacgcta tttgctacac aaattatgaa ttcaaatcac gctaaagcat cagtgacaga
     6481 gagtgttgac aaaaaatttg tagttccaga atcaggaatt aataaaatta ttccagctta
     6541 cgatgaattt aagaattcgc caaaagtaaa tgttagtaat ttaactgaca ataaaaactt
     6601 tgtagcttct gaagataaat tgaataagat tgcagattca tcggcagcta gtaaaattgt
     6661 agataaaaac tttgtcgtac cagaatcaaa gttaggaaac attgtgccag agtacaaaga
     6721 aatcaataat cgcgtgaatg tagcaacaaa caatccagct tcacaacaag ttgataagca
     6781 ttttgttgct aaaggcccag aagtaaatag atttattacg caaaacaaag taaaccacca
     6841 cttcattact acgcaaaccc actacaagaa agttattact tcatacaaat caacacatgt
     6901 acataaacat gtaaatcatg caaaggattc tattaataaa cactttattg ttaaaccatc
     6961 agaatcgcct agatatacac atccatctca atctttaatt atcaagcatc attttgcagt
     7021 tcctggatat cacgcgcata aatttgttac accagggcat gctagcatta aaattaatca
     7081 cttttgtgtt gtgccacaaa taaatagttt caaggtaatt ccaccatatg gtcacaattc
     7141 acatcgtatg catgtaccaa gtttccaaaa taacacaaca gcaacacatc aaaatgctaa
     7201 agtaaataaa gcatatgact ataaatactt ctattcttat aaagtagtta aaggtgtgaa
     7261 gaaatatttc tcattttcac aatcaaatgg ttataaaatt gggaaaccat cattaaatat
     7321 caaaaatgta aattatcaat atgctgttcc aagttatagc cctacacact acgttcctga
     7381 atttaagggt agcttaccag caccacgagt ataaaaattg gcactaagtt tacgagatat
     7441 gataaatacc tattatttta aatatagtct acaatctatg tggttgtagg ctgtattttt
     7501 tgcagtttat caataaacac ccatcaacaa attataccgt ttttctactt tgaaagttgg
     7561 aagtaacata atcttaaata aatatattat taattaagat aaatataata ctcaagatta
     7621 ttgttaatag tttgttcatc gcaagttaat tattgtttct aaaatattgg tatataattt
     7681 tcaatggcga agaaaacagg gtgaaaaagt cggtttttat atcaaagcaa ataagggagc
     7741 ataaacaaat gaaaaggaaa gtattagtac taacaatggg tgtaatttgt gcaactcaat
     7801 tatggcattc taatcacgca aacgcattag taacagagag tggcgcaaat gatactaagc
     7861 aatttactga aattgtatcg gaagagaaag ttataacagt tgaacatgct caaattaata
     7921 tttttcaatc taatagcaat tcaaacttga tggagttcaa catattaaca atgggcggta
     7981 aatcaggagc tatggttggt tatagtgaaa ttgactcatc acatttcaca gaccgtgaca
     8041 aacgcgttat tagacgtgat catgttaaag aagcacaaag cttagtagag aactataaag
     8101 atacacaaag tgctgatgct aggatgaaag ccaaacaaaa agttaacaca ttaagcaaac
     8161 cgcatcaaaa ctatttcaat aaacaaattg ataaggttta taatggatta caacgctaat
     8221 ccaaagtaaa ttataagtta tacatctcgt ttttaaatga caatttatcc ccgtaaatat
     8281 tataaataat cttttcaaat tccacataga tatagagaca ctaataaacc tctttgtctc
     8341 gatatgatag tctgcaacga ttcatgttgt aggcttttta attttacaaa taaggctaaa
     8401 tatataagtt ctggcaccta aaatatagaa aat
//
LOCUS       DALAGD010000112         8390 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.113, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000112 DALAGD010000000
VERSION     DALAGD010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8390
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.113"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(<1..276)
                     /locus_tag="O2701_002373"
     CDS             complement(<1..276)
                     /locus_tag="O2701_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS257 family transposase"
                     /protein_id="HCZ4294246.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHH
                     STVYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRA"
     gene            538..723
                     /locus_tag="O2701_002374"
     CDS             538..723
                     /locus_tag="O2701_002374"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294247.1"
                     /translation="MNKLEKWSLVFIIFTFLSSLALLYFKYNFSSVMPVLSLSILTIL
                     ILLTSIITVVSVNDEDT"
     gene            complement(946..1524)
                     /locus_tag="O2701_002375"
     CDS             complement(946..1524)
                     /locus_tag="O2701_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCZ4294248.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPG
                     LDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNG
                     TPIKTIAEQWQVSRTTIYRYLNKLEAKENKNK"
     gene            complement(1788..2168)
                     /gene="blaI"
                     /locus_tag="O2701_002376"
     CDS             complement(1788..2168)
                     /gene="blaI"
                     /locus_tag="O2701_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HCZ4294249.1"
                     /translation="MTNKQVEISMAEWDVMNIIWDKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
     gene            complement(2158..3915)
                     /gene="blaR1"
                     /locus_tag="O2701_002377"
     CDS             complement(2158..3915)
                     /gene="blaR1"
                     /locus_tag="O2701_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HCZ4294250.1"
                     /translation="MAKLLIMSVVSFCFIFLLLVFFRYILKRYFNYSLNYKVWYLTML
                     AGLIPFIPIKFSFIKFNNVNNQAPTVESKSHDLNHNINTTKPIQEFTTDIHKFNWDSI
                     DNICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIV
                     IRKAEAIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFN
                     IFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLKTPFSN
                     KNILSHSFNGKKSLLKSRLINIKEADLKKQSKLILIFICIFTFFIMIIQSQFLMGQSL
                     TDYNYKKPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTYK
                     IYLALFGLDRHIISDKNSRMSWNHKHYLFESWNKEQDLNTAMQNSVNWYFERISNQIP
                     KNYTAAQLKQLNYGNENLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQ
                     LSSSLLIKKNEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGNPSGK
                     NAELISEKILKGMGVLNDQ"
     gene            4022..4867
                     /gene="blaZ"
                     /locus_tag="O2701_002378"
     CDS             4022..4867
                     /gene="blaZ"
                     /locus_tag="O2701_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="HCZ4294251.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALN
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMFNNKNGDTLIKDGVPK
                     DYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
     gene            complement(5393..5812)
                     /locus_tag="O2701_002379"
     CDS             complement(5393..5812)
                     /locus_tag="O2701_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCZ4294252.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLIN
                     SNLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWP
                     TLEKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
     gene            5936..6907
                     /locus_tag="O2701_002380"
     CDS             5936..6907
                     /locus_tag="O2701_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCZ4294253.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKD
                     MLALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEY
                     AQVPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGG
                     VGSVAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLD
                     TVGSNVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIR
                     EDIWSKLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
     gene            complement(7079..7171)
                     /locus_tag="O2701_002381"
     CDS             complement(7079..7171)
                     /locus_tag="O2701_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCZ4294254.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
     gene            complement(7328..7675)
                     /locus_tag="O2701_002382"
     CDS             complement(7328..7675)
                     /locus_tag="O2701_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ4294255.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKK
                     LKIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDD
                     EIREFFSKNHEGF"
     gene            complement(7694..8311)
                     /gene="cadD"
                     /locus_tag="O2701_002383"
     CDS             complement(7694..8311)
                     /gene="cadD"
                     /locus_tag="O2701_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium resistance transporter CadD"
                     /protein_id="HCZ4294256.1"
                     /translation="MIQTVVAAAVLYIATAVDLLVILLIFFARAKTRKEYRDIYVGQY
                     LGSIILILVSLFLAFVLNYVPEKWILGLLGLIPIYLGIKVAIYDDCEGEKRAKKELNE
                     KGLSKLVGTVAIVTIASCGADNIGLFVPYFVTLSVTNLLLTLFVFLILIFFLVFTAQK
                     LANIPGIGEIVEKFSRWIMAIIYIALGLFIIIENDTIQTILGFIF"
BASE COUNT         2972 a         1227 c         1100 g         3091 t
ORIGIN      
        1 ggcacgatat aaatagctcc attttccttt tattttgatg tacgtctcat caatacgcca
       61 tttgtaataa gcttttttat gctttttctt ccaaatttga tataaaattg gggcatattc
      121 ttgaacccaa cggtagaccg ttgaatgatg aacgtttaca ccacgttccc ttaatatttc
      181 agatatatca cgataactca atgcatatct tagatagtag ccaacagcta cagtgataac
      241 atccttgtta aattgtttat atctgaaata gttcatacag aagactcctt tttgttaaaa
      301 ttatactata aattcaactt tgcaacagaa cctcatttac tatatgggca tctctttcat
      361 tattaataca aagctttgta tctccgatat taggcaaagg tgtaaacgaa ttaggtgata
      421 ctataggatt caacattata ttaatcgttt ctttaataat gattatcttc ttatttttta
      481 taaatcgtag taaaaatatc tcctatgatt atataaaagt tcaaagaggt gaagccaatg
      541 aataaattag aaaaatggtc tttggtattt attattttta catttctctc ttcattagca
      601 cttttatatt tcaaatataa ttttagttcg gttatgccag tcctaagtct ttctatttta
      661 actatactaa tattgttgac gagtattatt actgtagttt cagttaatga tgaagatact
      721 taaaacgttc attagtgaaa caggtattat attaaaaaag cgtacgaatt tgtacgcttt
      781 tttcttgtgt tattattaaa atctcataac aggaaaatat tatgagattt tagacgctgt
      841 cagaacgtct cttttacatc gagtgacatt gaatcaaagt ctttgtatgt taatgatata
      901 taaaattatt tagtagattc attatagtaa gctagattag ttgctctact tgtttttatt
      961 ctcttttgcc tctaatttat tgagataacg ataaatagtt gtacgtgaaa cttgccactg
     1021 ctctgcaatt gttttaattg gcgtaccatt atcataaagt gtcttaagta agtttaaatc
     1081 ttgtttattt aatttttcag gtctacctcc atatcggcct ctagcacgtg ctgcaattcg
     1141 accggctgat gatcgctcta aaattaaatt acgttcaaac tctgcaaatg ctgcgaataa
     1201 atgaaataat aattgtcctg tagaacttga tttatccatc gttatatttt cttctaaact
     1261 atgaaaactc acacctcgtt cgttaagttc attaactaac gtgattaaat cctccatgtt
     1321 ccgtcctagt ctatctaatc tccaaacaac aatcgtatct cctgacctcg caaattcaat
     1381 tgctttatct aaaccaggtc gcttactttt tgaaccactc atatggtcat taaatatctt
     1441 ttcacaacca tatgtattta atcgatcctc ctgtaaattc aaattttgta atccagttga
     1501 aactcttgca taacctattt tcaaaataca gacctcctat atacaccttt agtgtaccat
     1561 aacttgatta cgaggtcttt tgtgaacata gattttttgt tgggtttttg aacacataat
     1621 tctatgtaat acttacttat ttagacaaaa aacaagagtg tacagaaaat ggttattttc
     1681 tggacactct catctttaat aatatatttt aaatgtcatt aacaaaacta atttaataag
     1741 agtcaagtat ggtttacata tgtattatgt ttgaaacata cctctaatta ctttttacta
     1801 atatcattta aaatgtctcg caattcttca atttctttgt tatttaattc ttcatttttc
     1861 gcaaaattca gcactaaact tttcatatcc cctccataca gtttattaag aaaggtttta
     1921 gcagttttca ttttaatatc gtcttcttta atatttgatg agtaaaaata aatattttct
     1981 gatttgtatc gttttataat ctctttttta tatagtcttg tgattaatgt tctaatcgtt
     2041 ttatcactaa cttctttata tttttgaatt tcaactacaa tttcattagc tgatactgat
     2101 tttttatccc atattatatt cataacatcc cattcagcca tagatatttc aacttgctta
     2161 ttggtcattt aaaacaccca ttccttttaa tattttttca cttattaatt cagcattttt
     2221 cccagacggg tttccatctg ataaatgtgt agcaaaataa tacttatcat gatttgtaat
     2281 tacgtaacct acaaaccacc cattattata cttcccgttt actatacctg tacctgtttt
     2341 cccatacagt tcatactttt catttttctt aatcaataat gaagaagata attgattctt
     2401 tgctttttta ctaaaatggt tattttgttc catcatattt ttaaaaacta ttacttgttc
     2461 aagattagat attttcaaac tatcttccat ccaatagctt ttataacttc ccaaattttc
     2521 attaccataa tttaattgct tgagttgagc cgcagtatag ttctttggta tttgattact
     2581 aatacgttcg aagtaccaat taactgaatt ttgcattgct gtatttaaat cttgttcctt
     2641 attccaagac tcgaaaagat aatgtttatg attccatgac atacgtgaat ttttatcact
     2701 tataatatga cggtcaagtc caaacagtgc taaataaatt ttgtaagttg aatcaggaga
     2761 atatcttttt ctactttctt tttcattata aatatagtat ttgtctttct tcatgctgta
     2821 cataacgaaa gatccactat ttgaaccaaa atttttactt tcatctaaaa tttggtaatc
     2881 gctttgtaag ggctttttat aattataatc agttaaggat tgccccatta aaaactggct
     2941 ttgaattatc atgataaaaa aagtaaaaat acaaataaaa attagaatta attttgactg
     3001 ctttttcaga tcggcttctt ttatattaat taatctactt ttgagtaatg attttttacc
     3061 attaaatgaa tggcttaata tatttttgtt ggaaaatggg gtttttaata cggagtccat
     3121 aatagattcc gcataagttt taaattcatt tttgttaata ttattaagga caaatctatc
     3181 ggcttctact tcattgtcat gaattatctt tctttttaca atatgtacta atggattgta
     3241 actcataata atagagaaga tattaaagat aattaaatgt aaagtatctc tatttttagc
     3301 atgagcatat tcatgcagta ttatatattt tagtctttta tcaattacac ttttaaaata
     3361 tgaactagga atcaaaataa tatatttccc ataccaaaaa gttattggag attgaatagc
     3421 ctctgctttt cgaatcacaa tatttttttt atattgatgg ttgaaaagta tcgtatctat
     3481 tttatttttt tcattttcgt ttagataaag tgactgtttc tttaaatatt taagatataa
     3541 taaggctttc aaaaatttaa aacttaaaat aataactaaa actatccaaa taactgtgca
     3601 gatattatca attgaatccc aattaaactt atggatatct gttgtgaact cttgaatagg
     3661 tttggtggta tttatgttat ggttcaaatc gtgtgactta ctttcaactg tgggcgcttg
     3721 attattcaca ttattaaatt ttataaaaga gaatttaatt gggataaaag gaattaatcc
     3781 agcaagcata gttagatacc aaactttata atttaaacta taattaaaat agcgttttaa
     3841 tatgtaccta aaaaatacta acaatagaaa tataaaacaa aagcttacta cgctcattat
     3901 tagtaattta gccattttga caccttcttt caaatattta taataaacaa ttgacatcaa
     3961 tattacaatt gtaatattat tgatttataa aaattacaac tgtaatatcg gagggtttat
     4021 tttgaaaaag ttaatatttt taattgtaat tgctttagtt ttaagtgcat gtaattcaaa
     4081 cagttcacat gccaaagagt taaatgattt agaaaaaaaa tataatgctc atattggtgt
     4141 ttatgcttta aatactaaaa gtggtaagga agtaaaattt aattcagata agagatttgc
     4201 ctatgcttcg acttcaaaag cgataaatag tgctattttg ttagaacaag taccttataa
     4261 taagttaaat aaaaaagtac atattaacaa agatgatata gttgcttatt ctcctatttt
     4321 agaaaaatat gtaggaaaag atatcacttt aaaagaactt attgaggctt caatgacata
     4381 tagtgataat acagcaaaca ataaaattat aaaagaaatc ggtggaatca aaaaagttaa
     4441 acaacgtcta aaagaactag gagataaagt aacaaatcca gttagatatg agatagaatt
     4501 aaattactat tcaccaaaga gcaaaaaaga tacttcaacg cctgctgctt tcggcaagac
     4561 tttaaataaa cttatcgcaa atggaaaatt aagcaaaaaa aataaaaatt tcttacttga
     4621 tttaatgttt aataataaaa acggagacac tttaattaaa gatggtgttc caaaagacta
     4681 taaggttgct gataaaagtg gtcaagcaat aacatatgct tctagaaatg atgttgcttt
     4741 tgtttatcct aagggccaat ctgaacctat tgttttagtc atttttacga ataaagacaa
     4801 taaaagtgat aagccaaatg ataagttgat aagtgaaacc gccaagagtg taatgaagga
     4861 attttaatat tctaaatgca taataaatac tgataacatc ttatagtttg tattatattt
     4921 tgtattatcg ttgacatgta taattttgat atcaaaaact gattttccct ttattatttt
     4981 cgagatttat tttcttaatt ctctttaaca aactagaaat attgtatata caaaaaatca
     5041 taaataatag atgaatagtt taattatagg tgttcatcaa tcgaaaaagc aacgtatctt
     5101 atttaaagtg cgttgctttt ttctcattta taaggttaaa taattctcat atatcaagca
     5161 aagtgacaat agcattttac aagtattttt tattgttcga ttagggtaaa cctaaataat
     5221 acataaatta tattacaata tgtttattat agaggctaaa gtatcttaga aatttggata
     5281 gtaggttttt cgggaataaa agtatagttg gctttaatat ataattaagc taagctatca
     5341 tttatattta ctcgattata attcgcactt caactttttt ataacgtaaa aattaaaacg
     5401 tagttttatt tatctttagt aatatctttt taaacactac agtttcttca ttagttaaat
     5461 tagcgaagta atgattatta agttgatagg ctttcttttc taatgttggc cataagtctt
     5521 ttgctttatt tgtactgttt attagcacag ttcttctatt ttcattatca gtttgacgtg
     5581 ttattagatc ctttttggac atcttatcta atattccaga tattgttgaa ggttctaaat
     5641 ttaaagtatt accaatcgct ttaggtgtat tcaaattact attaatcaag caattcaaaa
     5701 caccatattc tgctggagta atattatagg gacttaataa acttttaaaa tgtttaaaaa
     5761 ctctattctg agaagtactc aacaaataat ttatacaatt atctaattcc acaactaaaa
     5821 cctctcttaa atcattagta ttgactcgct tatcatatca tgataagttt tgtaggaaaa
     5881 tagttttgca acaaaaacat acttctacaa aacaattgtg aaagaaggct aataaatgtt
     5941 caatgcggta gtagtgagaa aaagtgcatc aaatgttagt tataaagtag aaaaagttga
     6001 tgaatctcat ttatcgaagg gtgatgtatt agtaaaagtg gtatactctt caataaatta
     6061 taaggatatg ctagctttac aatataaagg cggtgtaatt agagattatc ctatgattcc
     6121 tggtatagat tttgcaggta ttgttgaatc aagttcgaat gataagttta aagaaggcga
     6181 taatgtatta gttactggtt atgatgttgg cgtgacacat acaggtggat tttcagaata
     6241 tgcacaagtg cctggagagt ggattgttcc tattccccaa gaaatgtcta ttaaagagtc
     6301 tatggtatat ggcacagccg gttttacggc agcattatca atttttgaac tagaaaaata
     6361 tggaatgaat atcaaatcac aacctgaaat tttagtgtct ggggctacag gtggtgtggg
     6421 cagtgttgct atacaaattt tacataaaat tggatatgaa aatatcactg ctatcgtaag
     6481 aaaagaaaag caaatagaaa tcgcaaaaaa attaggtgca aaacaagtaa taataattga
     6541 agatgataat aaaccgctta aaaaaggtat ttttgattat ttcttagata cagtggggag
     6601 taatgtaaca ttatacggtt taaaacgtct aaattatcaa ggtgttgcta ctgtatgtgg
     6661 aaatgtagca ggaaattcac ttaacgctaa tatattaccg tttattctta gaggtattaa
     6721 attaattggc attgattctg tatatgttga tcataatatc cgtgaagata tatggagtaa
     6781 gttagcaaat gaatggaata taggaaattc acttctatac aatgaaattg gatttgaaga
     6841 attctataaa acgatagatc agcttttaaa aggacaacat ttaggaagaa ctattttaaa
     6901 agtataagtg tgagtattca ttaatacatt tactattaat tcaatcattt tgcatttctc
     6961 tactaaccag tgttataatt tatataacga aaatgattaa caattaatgc accaatcccc
     7021 acactattgc agtagtgtgg ggattttttc gatgctggct tataaagtcg cctattaatt
     7081 atttgttgcg tttactaagc caataagtat aatacgcaac agcacagcca ctgatgactg
     7141 gtgctatgat gtgaacgaaa atgattagca aagtaatgca cctcctctct tcgtcaattg
     7201 acgcctgaga gataggcgac tctattatta taccatctgt gaatatacaa gcatatctac
     7261 ttccgataag tcgtcttatg ttatataact ttgtttttta tagtatcaag acaagaagaa
     7321 actcgtttca aaaaccctca tgatttttag agaaaaactc tcttatttca tcgtctttaa
     7381 taaaataata tgccatcttt ccatctttat aaaaatcaag tacctcattt ttatataaaa
     7441 gccttaaatg atgtgaagtt gaagcaacac tcattttcaa tatcaaagaa atatcacaaa
     7501 cacacaactc atcttcttta atcaaagata atatgatttt caatttcttc tcatcacaaa
     7561 ttttttctaa aatgttaagt aatttcttag atttatcatc ttctaaaaaa cttaaagcat
     7621 tgttaacttt atcctcatgt acacagatca catcacaagt attttcataa ctcatataaa
     7681 atcacaccct aaattaaaat ataaatccta aaattgtttg aatagtgtca ttttcaataa
     7741 taataaataa acctaaagct atataaataa tagccataat ccaacgacta aatttctcaa
     7801 caatttctcc aattcctgga atattagcta atttttgtgc agtaaacacc aagaagaaaa
     7861 ttaaaattaa aaagacaaac agagtaagta ataaattagt aacacttaat gtcacaaaat
     7921 acggaacaaa taaaccaata ttatcggcgc cacaacttgc tatcgtaaca attgcaaccg
     7981 taccaactaa tttagacaat cctttttcat tcaattcttt tttagctctc ttttctcctt
     8041 cacaatcatc ataaatagcc actttaattc caagataaat tggtattaaa cccaataaac
     8101 ctaatatcca cttttctgga acataattta atacaaaagc taaaaacaaa ctaactaata
     8161 ttaaaataat agaccctaaa tattgaccaa cataaatatc tctatattct tttctagtct
     8221 ttgctctagc aaaaaatatt aataaaatta ctaataaatc aactgctgta gcaatataaa
     8281 gaacagcagc agcaacaacc gtttgaatca taatacacct cattcaaaaa tatttttgaa
     8341 tgtattataa cttatatttt taaataatca taggtgcttt attccaattg
//
LOCUS       DALAGD010000113         8360 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.114, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000113 DALAGD010000000
VERSION     DALAGD010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8360
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.114"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..744
                     /locus_tag="O2701_002384"
     CDS             <1..744
                     /locus_tag="O2701_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E domain-containing protein"
                     /protein_id="HCZ4294257.1"
                     /translation="EITKDPVNELTEFGGEKIPQGHKDIFDPNLPTDQTEKVPGKPGI
                     KNPDTGKVIEEPVDDVIKHGPKTGTPETKTVEIPFETKREFNPKLQPGEERVKQEGQP
                     GSKTITTPITVNPLTGEKVGEGQPTEEITKQPVDKIVEFGGEKPKDPKGPENPEKPSR
                     PTHPSGPVNPNNPGLSKDRAKPNGPVHSMDKNDKVKKSKIAKESVANQEKKRAELPKT
                     GLESTQKGLIFSSIIGIAGLMLLARRRKN"
     gene            1128..2321
                     /locus_tag="O2701_002385"
     CDS             1128..2321
                     /locus_tag="O2701_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ4294258.1"
                     /translation="MLYLEVLKNRNFTYLLIGNFLRRSCFVLFSLQIIWFTVELTNQS
                     SLKLSMMVMSQTLPFIIFGIFGGAYSDKHNKKKILYLSDLILSLIIIIIPLLAITSNL
                     NYLTLLTISTAITIINCYTDPAFRAILPEIIDEEHLATSNALIDSLQRGSNIILPALI
                     GVIVILVGNVGVFFICSILLFLGFIFNALLKYTNNNMIDRHSKEDFSETWEFLKQSKE
                     IPFIIIIQFACILINTGLWRVVLPLFISNILKEGVGVYGLATSCLGIASLLMSLIMGL
                     LSEKRLIFKFSIGVLVWGIGLSIINVFPSVAILYIGATLLGLGQSIEGLTRSVAIQIK
                     TPNHLMGKVFSISSTSNYAADTLSLGLISIIIPLLSLSNIFILGGVIISILSLAGLKF
                     VKKSM"
     gene            2302..2535
                     /locus_tag="O2701_002386"
     CDS             2302..2535
                     /locus_tag="O2701_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294259.1"
                     /translation="MLRSLCKHYRILINAIKVGIEMKYKISLAYNLAIIIGSLIILCI
                     LISRGYDIYVILIPILTILASLINLFCDIKKHK"
     gene            complement(2643..2951)
                     /locus_tag="O2701_002387"
     CDS             complement(2643..2951)
                     /locus_tag="O2701_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294260.1"
                     /translation="MTVNIVFSIVFCISMVILGIYVAITKDFTLISFINQTAIADKHK
                     NQIAYIFTLCISLSAVFLMSSILSFEYDFIALAFLFLTIALLLIALFYVCFYKITKYP
                     "
     gene            complement(3095..3361)
                     /locus_tag="O2701_002388"
     CDS             complement(3095..3361)
                     /locus_tag="O2701_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294261.1"
                     /translation="MKGSKQILLIMGIISLIVLFIFTLFIMAQYAKHYEQKSDSSNAH
                     TLNSSSAIIEQHTMSNLASLDLYAPVRNITSSRDIHPIYFMTKD"
     gene            complement(3645..5453)
                     /locus_tag="O2701_002389"
     CDS             complement(3645..5453)
                     /locus_tag="O2701_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HCZ4294262.1"
                     /translation="MHFHTHLLYNTCEKYCLGAVFFYFWGVFLYNSLHKCKGFSFHVL
                     LSLTEILKGCLATMDKELWIERANDSLVKHFYEQQSDIEQREGFESKLTFGTAGIRGK
                     FGLGEGRLNKFTIEKLALGLARYLNAQTNSPTIVIHYDIRHLSTEFAQIIANVLANHQ
                     ITVYLPDTYKTTPELSFAVRNLNTTAGIMITASHNPKDYNGIKVYGSDGAQLSTDASE
                     LASRYIEEVGDPLQIDIPISKQNTSYIKPFPKSVTDDYMKHIQNMIGYIPKSDLQVVF
                     TSLHGTSVPIVPELLQSLNFNQFNLVEAQCKPDPNFSSVQSANPEDHRAFDQAVELAN
                     KSHADLLISTDPDADRLGIAERDAHGHITYFNGNQIGALLLNYRIQQTSQLRHRLMIQ
                     SIVSSELTKSLARYNNVEYKEVLTGFKFIAQEIRQLDDHQNMIFAFEESYGFLSEPFV
                     RDKDAVQIVPLIIKYASELKLYGKTLKDELEQIYQTVGRHEDTLFSHTLEGFEGKKKI
                     NAIMTKFRSNPPQEIQGLKVKAIEDYLTSEVYHLDKDTTSQINSPKSNVIRVLFDEGF
                     IALRPSGTEPKIKLYVSLKCPNFDDVAQKINAMIFS"
     gene            5570..5962
                     /locus_tag="O2701_002390"
     CDS             5570..5962
                     /locus_tag="O2701_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HCZ4294263.1"
                     /translation="MKKVINVVGAIIFSDNKILCAQRSEKMSLPLMWEFPGGKVEKNE
                     TEKDALIREIREEMKCDLIVGDKVITTEHEYDFGIVRLTTYKCTLNKELPTLTEHKSI
                     EWLSINELDKLNWAPADIPAVNKIMTEG"
     gene            5964..>8360
                     /locus_tag="O2701_002391"
     CDS             5964..>8360
                     /locus_tag="O2701_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HCZ4294264.1"
                     /translation="MSRLLNDFNQSLHKGFIDKHISHKGNYTPKLLVNNKNEKVLSTI
                     IDELQKCETFYFSVAFITESGLASLKAQLLDLSNKGVKGKILTSNYLGFNSPKMYGEL
                     LKLKNVEVRLTDIAGFHAKGYIFEHKDYSSMVIGSSNLTSNALKVNYEHNVLLSTMKN
                     GDLVDSVKSEFDLLWQKSTPLTEQWINSYKESFEYRSLEKLAEVEQTQMLLADKVKKS
                     VEIVPNLMQAEALRSLKAIRDKAKDKALIISATGTGKTILCALDVREVNPNKFLFIVH
                     NEGILNRAKEEFKKVLPIKNDSDFGLLTGKHRDVDAKYLFATIQTLSRDDNFKQFDEN
                     EFDYIVFDEAHRSAASTYQRVFNYFKPKFMLGMTATPERSDELSIFELFDYNIAYEIR
                     LQAALESDILCPFHYFGVTDYVHQGIKEDDVTKLRYLTSDERVNYIIQKTDYYGYSGE
                     ILQGLIFVSSKKEAYDLADKLSSKGIKSVALTGDDSVNYRQIVIEKLKEGKINYIITV
                     DLFNEGIDIPEVNQVVMLRPTESSIIFIQQLGRGLRKSSNKEYVTVIDFIGNYKTNYL
                     IPIALSGDQSQNKDNYKKFLTNNDSINGVSTINFEEVAKKQIYNSLDAVSLNQNKLIL
                     KAYEEVENRLGHMPLLMDFIQQHSIDPSVIFSKFSNYYEFLVRYKKIDTLLTENESKN
                     LVFFSRQIAPGLKRIDSLVLEELLKNELTYDELKNKMLNEVKDITEDDIDTSLRILDF
                     SFYNAGIEKIYGSPIIERNERMIRLSDAFTNALSNQTFNMFLEDLIELSKYNNEKYQK
                     G"
BASE COUNT         3055 a         1107 c         1428 g         2770 t
ORIGIN      
        1 gaaatcacaa aagatccagt taatgaatta acagaattcg gtggcgagaa aataccgcaa
       61 ggtcataaag atatctttga tccaaactta cccacagatc aaacggaaaa agtaccaggt
      121 aaaccaggaa tcaagaatcc agacacagga aaagtgatcg aagagccagt ggatgatgtg
      181 attaaacacg gaccaaaaac gggtacacca gaaacaaaaa cagtagagat accgtttgaa
      241 acaaaacgtg agtttaatcc aaaattacaa cctggtgaag agcgagtgaa acaagaagga
      301 caaccaggaa gtaagacaat cacaacacca atcacagtga acccattaac aggtgaaaaa
      361 gttggcgagg gtcaaccaac agaagagatc acaaaacaac cagtagataa gattgtagag
      421 ttcggtggag agaaaccaaa agatccaaaa ggacctgaaa acccagagaa gccgagcaga
      481 ccaactcatc caagtggccc agtaaatcct aacaatccag gattatcgaa agacagagca
      541 aaaccaaatg gcccagttca ttcaatggat aaaaatgata aagttaaaaa atctaaaatt
      601 gctaaagaat cagtagctaa tcaagagaaa aaacgagcag aattaccaaa aacaggttta
      661 gaaagcacgc aaaaaggttt gatctttagt agtataattg gaattgctgg attaatgtta
      721 ttggctcgta gaagaaagaa ttaaaataat tcataattta aataatagtt gatttgcatt
      781 cactatattt agtttgttaa aaacaaccta gaatatgatg agaatgatat acaccccaaa
      841 agttggattg aaagacctaa ctttaagggg tgtatttttg ttataaattt gatttttttg
      901 ataatatttt catatttaat ataatcataa ttagtagaat ataacctctg agtagtcatt
      961 ttgggggtta tgtatggtgt ttcaagtagt atgtatattt cgaaataatt gatatttaac
     1021 tgactaaatt atattagaat taattgcgct ttggataaag agagtttaga agaaggtcta
     1081 aaaaagctgg tggatgcatt caattatttt tatgagaggt gataaatatg ttgtatttag
     1141 aagttttgaa aaatagaaac tttacatatt tattgattgg taatttctta cgtcggagtt
     1201 gttttgtgct gttttcgtta caaataattt ggtttactgt tgaattgaca aaccaatcat
     1261 ctttaaaatt atcaatgatg gttatgagtc aaacacttcc atttattata tttggtattt
     1321 tcggtggcgc atattctgat aaacataata aaaagaaaat actttatcta tctgatctta
     1381 tactaagttt gattataata attattcctt tacttgcaat aacatcaaat ttaaattatc
     1441 tgacgcttct aaccatatct acagctatca ccattattaa ctgctatacg gatcctgcgt
     1501 ttagggcaat tcttcctgaa attattgatg aggagcattt agcaacaagt aatgcgttaa
     1561 tcgatagttt gcaaagaggc tcaaatatta ttttacctgc tttaattggt gtcattgtaa
     1621 tactagttgg taatgttggc gtctttttta tttgtagtat attgctattt ttaggattta
     1681 tttttaatgc acttttaaaa tatacaaata acaacatgat tgatagacac tcaaaagaag
     1741 atttttctga aacttgggaa ttcttgaaac aatctaaaga aattccattt attataatca
     1801 tccaatttgc ctgcatattg attaatactg gcctttggcg tgttgtatta ccattgttta
     1861 tttccaatat attaaaagag ggcgttggtg tatatggact agctacatca tgtttaggta
     1921 tagcatcttt attaatgtca ttgattatgg gtttgctgtc agaaaagcga ttaatcttta
     1981 aatttagtat tggtgtttta gtttggggaa ttggcttatc gattattaat gtgtttccaa
     2041 gtgtggcgat attatatata ggtgcaacat tattgggact tggacaatct atagagggac
     2101 taacaagatc agttgccatt caaataaaaa cgccaaatca tttgatgggg aaagtattta
     2161 gtatctcatc tacatcgaat tatgcagcgg atacattgtc tttaggactg ataagtataa
     2221 ttataccttt actaagctta agtaatatat ttatattagg aggtgtaatt atttcaatac
     2281 ttagcttagc tgggttaaag tttgttaaga agtctatgta aacattatag aattttaata
     2341 aatgctatta aggtgggaat tgaaatgaaa tataaaatta gtttggctta taatttagca
     2401 attataatag ggtcgctaat tatattatgt atactaatat ctagaggtta tgatatttat
     2461 gtaatcttga ttccaatttt gactatttta gctagtttaa tcaatttgtt ttgtgatatt
     2521 aaaaagcata aatgattcta tattaatgca actttgtatt cgtacaaggt tgtatttttt
     2581 tcgcaaacaa ttacttactg acagcgcatc aataaatcta accaacacac tatcacaaac
     2641 atttatgggt attttgtaat tttataaaag caaacgtaga ataatgcgat aagtaataat
     2701 gctatcgtta aaaataagaa tgctaatgca ataaaatcat attcaaagct tagaattgaa
     2761 gacattaaaa atactgcact caaacttata catagcgtaa atatatatgc aatttggttc
     2821 ttgtgtttat ctgcaatggc cgtttgattt ataaaagaga ttagtgtgaa atctttagtt
     2881 attgcgacat aaatgcctaa tataaccata cttatgcaga atacgatact aaatacgatg
     2941 ttgacggtca ttgttgcacc ttcctgatag aatataatgt actgtctttt agtaatggat
     3001 gcgtttctta tatatgtatg tttctaaaaa acagtacgtt tttatcattt tttaatttat
     3061 tagttgaatg aatggggtta tgaaaaagga agttttaatc ttttgtcatg aaataaatgg
     3121 gatgaatatc acgactagaa gtaatgttac gaacaggagc gtataaatct agagacgcta
     3181 aattcgacat agtatgttgc tcaattatgg ctgatgatga atttaaagta tgtgcgttgg
     3241 aactgtcgga tttttgttca taatgttttg catattgcgc catgatgaat agtgtaaaaa
     3301 taaataaaac aataagagat ataatgccca taatcaaaag tatttgttta gagcctttca
     3361 ttatttcaca tcctttctaa aatatatttg taactaaatt taaaatagtt atttttgtaa
     3421 ttctaaacct ttttcatcgc gaaaacaatt aaataggtcg cggtattaat tattatatta
     3481 ttaccgctta atatgaaaaa tacatgaaaa ttaattttct aatatacttt tgaaaaatta
     3541 ttacaaatta gccccttcaa aacgcgaaaa cataaggatt ctagtttcaa aagggctgat
     3601 aagcataaaa tgaaatgtaa tatttcgatg tataaaattt ttaattagct aaaaatcatc
     3661 gcattaattt tttgagctac atcatcaaaa ttcggacatt ttaacgacac atataattta
     3721 attttaggtt cagtaccaga aggacgtaaa gcgataaatc cttcgtcaaa taagacacga
     3781 ataacatttg atttaggaga attaatctgc gacgttgtat ctttatccaa atgataaacc
     3841 tcgctagtta aataatcttc aattgctttc actttgagtc cttgaatctc ttgcggtgga
     3901 tttgaacgga atttggtcat tattgcatta attttctttt tcccttcaaa tccttctagc
     3961 gtatgcgaga ataatgtatc ctcatgtcta ccaacagttt gataaatctg ttctaattca
     4021 tctttcaatg ttttgccata taattttaac tcagaagcgt attttataat gagtggcaca
     4081 atttgtacgg catctttatc acgtacaaaa ggctctgata gaaaaccgta actctcttca
     4141 aatgcgaaaa tcatattttg atgatcatcc agttgtctta tttcctgagc aataaattta
     4201 aagcccgtca gcacctcttt gtattcaaca ttattatagc gtgctaatga ctttgttaac
     4261 tcactactta caatagattg aatcattaag cggtgacgta attgagacgt ttgttgaata
     4321 cgataattaa gtaacagcgc accaatttgg ttgccattaa aatatgtgat atgaccgtgt
     4381 gcatcacgtt cagcaatacc aagtcgatct gcatcagggt cagttgatat taataagtct
     4441 gcatgacttt tatttgcaag ttcgactgct tgatcaaaag cgcgatgatc ttcaggatta
     4501 gcactttgaa ctgagctgaa attcggatca ggtttacatt gtgcttctac aagattaaat
     4561 tgattgaaat tcaaagattg taataactca ggtacaatcg gaacacttgt accatgcaaa
     4621 ctagtgaaca caacttgtaa atcagattta ggaatatagc caatcatatt ttgaatgtgt
     4681 ttcatatagt catcagttac agatttcgga aaaggcttga tataagatgt attttgttta
     4741 gaaataggta tatcaatctg taatggatcg ccaacttctt cgatataacg acttgcaagc
     4801 tcagatgcat cagtcgataa ttgcgcacca tcagaaccat atactttgat gccgttatag
     4861 tctttcggat tatgacttgc tgtaatcata atgccagcag tagtattaag attgcgcacc
     4921 gcgaacgata attccggtgt cgttttatat gtatcaggta aataaactgt tatttgatgg
     4981 tttgctaata cattagcaat tatttgggcg aattcagttg aaagatgtct aatatcataa
     5041 tgaatgacta ttgttggact gtttgtttgg gcatttaaat aacgcgctaa acctaatgcc
     5101 aatttttcaa tagtaaactt attaagtcga ccttcaccaa gaccgaattt tccgcgtata
     5161 cccgcagtac caaatgttaa tttactttca aaaccttctc gctgttcaat gtcagattgc
     5221 tgctcataaa aatgtttaac taaactatca ttagctcgtt ctatccataa ttctttatcc
     5281 attgttgcta aacatccttt caaaatctca gttagactta ataaaacatg aaaactaaag
     5341 cccttacatt tatgtaatga attataaaga aatacgcccc aaaagtaaaa aaacacagcc
     5401 ccaagacaat acttttcaca agtattatat aatagatgtg tatgaaaatg catggagtag
     5461 agcgttcatg tgaatgtaaa atattaattt ttaatattga tttatatata gttttgtaat
     5521 ttttgataga ataacaatta agatgatttt aaattgaagg gtgagtctta tgaaaaaagt
     5581 aatcaatgta gtaggagcta ttattttttc tgataacaaa attctttgtg cacagagaag
     5641 tgaaaaaatg agtctgcctt taatgtggga atttcctggc ggtaaggttg aaaagaatga
     5701 aactgaaaaa gacgctttga ttagagaaat tagagaagaa atgaaatgtg atttaattgt
     5761 tggagacaaa gttataacta cagaacatga atatgatttt ggaattgtta ggttaacaac
     5821 atacaaatgt actttaaaca aagagttacc aactctaact gaacataaga gtattgaatg
     5881 gttgtcaata aacgaacttg ataaattaaa ttgggcccca gcggatatac cagccgttaa
     5941 taaaattatg actgagggat aatatgagta gattactaaa tgatttcaat caatctttac
     6001 ataaagggtt tatagacaaa catatttctc ataaagggaa ttatacaccg aagcttttag
     6061 taaataataa aaatgaaaaa gttctttcaa caattataga tgaacttcaa aaatgtgaaa
     6121 cgttttattt ttctgtggct tttataacag aaagcggttt agccagctta aaggctcaac
     6181 tattagattt aagcaataag ggtgttaaag ggaaaatatt aacatctaat tacttagggt
     6241 ttaatagtcc taaaatgtat ggagaattac ttaaattaaa aaatgtagag gtaagattaa
     6301 ctgatattgc tggattccat gccaaaggat atatttttga gcataaggat tatagttcta
     6361 tggtaatagg aagctctaat ttaacatcta atgcgttaaa ggttaattat gagcataatg
     6421 ttttactgtc tactatgaaa aatggcgatt tagttgatag tgtaaaaagt gaatttgact
     6481 tgttatggca aaagagtacc ccactgactg agcaatggat taattcatat aaagaatcat
     6541 ttgagtaccg ttccttagaa aaattagcgg aggttgaaca aactcaaatg ctgctagctg
     6601 ataaggtgaa aaaatcagtg gaaattgtac ccaatttaat gcaagcagaa gcattaaggt
     6661 cattaaaagc tattagggat aaagccaaag ataaggcgtt aataatatct gcaactggta
     6721 caggtaaaac gattttatgt gcattagatg ttagagaagt aaaccctaat aaatttttat
     6781 ttattgttca taatgagggg attttaaata gagctaagga agaatttaaa aaagtattac
     6841 ctataaaaaa tgatagtgat tttggattgt tgacaggaaa acatagagat gtcgatgcca
     6901 aatatttatt tgcaaccatt caaacattat ctagagatga taattttaag caatttgatg
     6961 aaaatgaatt tgattatatt gtttttgatg aagcacatcg ttctgctgca tctacttatc
     7021 aacgagtatt taactacttc aaacctaagt tcatgctggg aatgactgct acaccagaaa
     7081 gatcagatga attaagtata tttgaactgt ttgattataa tattgcatat gaaataaggt
     7141 tacaagcagc attagaaagt gatattttat gtccatttca ttattttggt gtgactgatt
     7201 atgtacatca aggtattaaa gaagatgatg taactaaact gagatattta acttctgatg
     7261 aaagagttaa ttatattatt caaaagacag attactatgg atattcaggt gaaattttac
     7321 aaggattgat ttttgtaagt agcaaaaaag aagcttatga tttagcggat aaactaagtt
     7381 caaaagggat taaaagtgtt gctttaacag gagatgatag tgttaattat cgccaaattg
     7441 taatagaaaa attaaaagaa ggtaagatta actatattat aactgtagat ctttttaacg
     7501 aaggtattga tattccagaa gtcaatcaag ttgtaatgtt gagaccaaca gagtctagta
     7561 ttatattcat tcagcaactt ggtagaggat taagaaaaag ttcaaataaa gagtatgtta
     7621 cagtaattga ttttattggt aattataaga caaattattt aattccgatt gcgctttctg
     7681 gggatcaatc gcaaaataaa gataattata agaagttttt aacgaataac gattcgatta
     7741 atggagtatc tacaattaat tttgaagaag ttgctaaaaa acagatttac aattcattgg
     7801 atgcagtatc attaaatcaa aataaattaa tattaaaagc ttatgaagaa gttgaaaata
     7861 gattgggaca catgccgtta ctaatggatt tcatacaaca acattctata gatccaagcg
     7921 ttatattttc taaatttagt aattattacg agttcttagt gagatataaa aaaatagata
     7981 cattattgac ggaaaatgaa tctaaaaatc tggttttctt ttcaaggcaa atagcacctg
     8041 gattgaaaag aatcgatagt ttagttttag aagaactttt aaaaaatgaa ttaacatatg
     8101 atgaattaaa aaataaaatg ttgaacgaag ttaaggatat aacagaagat gatatagata
     8161 cttcattaag aattttagat ttttcatttt acaacgcagg aattgaaaaa atctatggaa
     8221 gtcccattat agaacgtaat gaaagaatga taagactaag cgatgcattt actaatgctc
     8281 taagtaatca aacatttaat atgtttttag aagatcttat tgaattatca aagtataaca
     8341 atgaaaaata ccaaaaaggt
//
LOCUS       DALAGD010000114         8223 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.115, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000114 DALAGD010000000
VERSION     DALAGD010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8223
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.115"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            127..942
                     /locus_tag="O2701_002392"
     CDS             127..942
                     /locus_tag="O2701_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002491360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HCZ4294265.1"
                     /translation="MHYIKFIESKDNTKLYMKVNDIQDAKANIIIAHGVAEHLDRYDE
                     ITAYLNEAGFSVIRYDQRGHGRSEGKRAFYSNSNEIVEDLDAIINYVKSNFEGKVYLI
                     GHSMGGYTVTLYGTKHPNTVNGIITSGALTRYNNKLFGNPDRNISPDTYIENNLSEGV
                     CSDLEVMEKYKLDDLNAKQISMGLVFSIMDGVRYLKDNAQQFTDNILILHGKEDGLVS
                     YVDSLQLYQEIGSAHKSLHIYDRLEHEIFNESSYNRTIFNEVIEWLETELTYN"
     gene            1264..2442
                     /locus_tag="O2701_002393"
     CDS             1264..2442
                     /locus_tag="O2701_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016913246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCZ4294266.1"
                     /translation="MSKRSSVVAHYKNGKFQKEFFIQNNKNGYNYLLKYLNDLDHPQL
                     IFESTGIYSRGMERFCCVNQINYIQMNPLEAKFKTSALRSWKTDQADAHKLACLGPTL
                     KQTGSLPIHELIFFELRERARFHLEIENEQNRLKFQILELLHQTFPGLERLFSSRYSI
                     IALNIAEIFTHPDVVLDIDKDVLITHIFNSTDKGMSMDKATKYALQLRVIAQESYPNV
                     DRHSFLVEKLRLLIQQLKQSIHHLKQLDDAMIQLAQQLDYFENIHSIPGIGKLSTAMI
                     IGEIGDIKRFKSNKQLNAFVGIDIKRYQSGHTHCRDTINKRGNKKARKLLFWVIMNII
                     RGQHHYDNHVVDYYYKLRKQPNEKPHKTAIIACINRLLKTIHYLVMNHKLYDYQMSPH
                     "
     gene            2702..3394
                     /locus_tag="O2701_002394"
     CDS             2702..3394
                     /locus_tag="O2701_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002490836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCZ4294267.1"
                     /translation="MTKTYDVWWQKGQESDDDMARDHQEAWERTIKMLDTSDIEGKTI
                     LDVGCNQGGFLRQLYDTTPFKKGVGIDLARLSLEKAETLKGQRPLTYYLTDKPQETKH
                     VFDTAVSTSVLYLIEDIPQHAKDLKEVLKPGGVYYASFADLTNNPSRQFMDDTINQYG
                     ATPSQNHSLKHIVDSFVDAGFEVAVMKEHVPDVIDLTHYSDFYLSPNDYLQTLYEESF
                     LIKASVKEGTEK"
     gene            3391..4944
                     /locus_tag="O2701_002395"
     CDS             3391..4944
                     /locus_tag="O2701_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HCZ4294268.1"
                     /translation="MRKCVLMTVAASATLLLAGCGNGQKEDKDVTVSLPTEAKADKLD
                     AQGYDAAMPVYSAVYDALVKYDKDKGIKAGLADKWSVDESGKVYEFHLKKNVKFSDGS
                     ALDAKDVKFSIDRAKAMNKDSTVETLKKLDKVVVKNEHVVQIRLKSPSNQVLNELTQV
                     RPLRIMSPHSVEDGKVNGKFEKAIGTGAFVVDKTGKEKTTMKPNKYFDNGHPVNYHLA
                     FQTIEDGDSRNSAVQSGSVDISGGALGMLSDEQIKQDKKNKNLTIEDRPSTVSHFMAF
                     NPKNDVLNQRTIREAISKSIDAKDIAGKSVNGLFQKNVQFVTKNNQQPHDYDMKAAER
                     LLKSEGYHKNDDGIFEKNGKPLSFNLVIQTAEFPNWKDKAEKVQRQLKQAGIKLNVKT
                     LDSQSYYDTLWTKKDYDLIFYRTYSDALMPYNFISSVFKNNDGQPGVLADDETLTKQL
                     DDFPSTVSKEDQQCSFDDIFKHFNQQYYGVPIAYPNETFVVSDKVKQFKFSGLTDAPI
                     DYKALKVNE"
     gene            4947..5903
                     /locus_tag="O2701_002396"
     CDS             4947..5903
                     /locus_tag="O2701_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019165742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4294269.1"
                     /translation="MLKRTIKFILYLIVSSFIIFILVEKTSGNPAILYLQRHGYTSIT
                     QDNIEAAQHQLGLGQHVLLRYIDWVGHALTGNLGYGFSTNEAVTAMIMEAIVPTLVLI
                     IVSSCIMLPFGYIVGYFVGTRPHTRYANGIRGFAQVMTSMPEYWLAILFIYYLGVRWQ
                     LLPFVGSDSWQHFVLPIFTIVVIEGCHILLMTAHLITQTLDQDAYQLAQLRHFSLKAR
                     IIVQIKEIFAPLMTISINSIIHLIGKAVILEVIFSMSGIGKLLINAINQRDYPLIQGI
                     VIFIIVFIMLMNYLGDVIILKNEPRLRRRHTQQSGNEKRGTM"
     gene            5903..6670
                     /locus_tag="O2701_002397"
     CDS             5903..6670
                     /locus_tag="O2701_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002469402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4294270.1"
                     /translation="MKKYQTYIAIGSLLSLMVVLITYGLMQDTQHLNPLESPNGQHWL
                     GTDQLGRDFLVRLIVGSLVTLSLTGIVILLSVCMGLIFGLIAGIERRWLDQIIMFVAD
                     MLLAIPSFIIALVILSLVSNSMIGLILALTIGWIGRYLRYFRNLTRDIQKRPFVQYAR
                     LSGNSTFKTTVTHVIPHLLSSIFALVTADFGKMMLSISGLAFLGLGIKPPTPELGTIL
                     FDGKSYFNGAPWLFFFPGVLLGGFALLCQIINKKITQ"
     gene            6637..7404
                     /locus_tag="O2701_002398"
     CDS             6637..7404
                     /locus_tag="O2701_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4294271.1"
                     /translation="MSNYQQKNNAVNTVVNVNQLSILDQEKVLLKDVDLTVTKGAFHC
                     IIGESGSGKSLLTRTILGMKQSQLCYQGDIDIDLTQTDAVFQDVQSNMFQNITLAKHF
                     QYIYEANRTHLTKQRIKEDVLQMMQLLGLRQGEQLLERYPFELSGGMAQRVAFIMSLI
                     RRPNYLFLDEPTSALDQENIKKFMHYLLRAQERYQMTIVFITHDINLVKDCATHISIM
                     QQGKLIENGEAASILTKPTHNYTKKLITIAHRRQPYA"
     gene            7397..8032
                     /locus_tag="O2701_002399"
     CDS             7397..8032
                     /locus_tag="O2701_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002490830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCZ4294272.1"
                     /translation="MLKIERLTKYIDTQLIFKEISCTINDQHLLISGESGCGKSTLAK
                     IIAGLDTDYQGELYLNGRLRESYTSKEWMKHIQYVPQYQRDTLNQRKTVLATLLEPLK
                     NYKVNKQRYTSSIEAVLDQCNLPHDILNHKVSTLSGGQFQRVWIAKALILEPEILILD
                     EATTNLDVINEEAILQMLISLKMTQLIIISHDTYVLSQFEGIQLQLNKLNN"
BASE COUNT         2909 a         1261 c         1550 g         2503 t
ORIGIN      
        1 taaaaaacat gggcttaaag ggttaattta tatagtatta ctgcaaacat tgatttgggt
       61 caaaaattag gggtattaaa aaaatgaatg aatcataatt ttatcaagct gattggagag
      121 gttaaaatgc attatataaa atttattgag tcaaaagata atacaaaact ttatatgaaa
      181 gtgaatgata ttcaagatgc aaaagcgaat atcattatag ctcatggtgt ggcagaacat
      241 ttagatcgtt atgatgagat aacagcatat ttaaatgaag cgggttttag tgttattaga
      301 tatgatcaaa gagggcatgg tcgttctgaa ggcaagcgtg ccttttatag caatagtaat
      361 gaaattgtcg aagatttaga tgcgataata aattatgtga agtcaaactt tgaaggtaaa
      421 gtttacttaa tcggtcatag tatgggtggt tatacagtca ctttatatgg aacgaaacat
      481 ccaaatacag tgaatggtat tataacttct ggagcattaa cacgttataa taataaacta
      541 tttggcaatc ctgatagaaa catatcacct gatacttata tagaaaacaa tttaagtgag
      601 ggggtatgtt ctgatttaga ggtaatggaa aaatataaac ttgatgattt gaatgcgaaa
      661 caaatctcta tggggctcgt cttttcaata atggatggtg ttaggtattt gaaagacaat
      721 gctcaacaat ttacagataa tattttgata ttgcatggca aggaagatgg gctagtaagc
      781 tatgtagatt ctttacagct ttatcaagaa ataggatcag cacataaatc attacacatc
      841 tatgatcgtt tggagcatga aatatttaat gaaagttctt ataatagaac tatttttaac
      901 gaagttattg aatggcttga aacggaatta acttataact aaaacagtat agttccgtgt
      961 atttgattat aagaaattat gaggatatta aacatactaa gattagctat gaagaaatct
     1021 atgacgatag attttttcat agctattttt tatagttata gagaggagta gactgtccag
     1081 actcttggat tttaaatccg taaaaaaaac aagtcagctt tactctcacc ttttgaaatt
     1141 cgtttgtagt atgttgggtt cttgaaaccg tgtataggaa aatgaaatga gaaaggttaa
     1201 gtaaagtttt tagcttctca actattcaaa ggaggttttt ttatcgatta cttaggtgtt
     1261 gatattagta aaaggagtag tgtagttgca cattataaaa atggaaaatt ccaaaaagag
     1321 tttttcatcc aaaataataa aaatggctac aattatttac tcaagtattt gaatgactta
     1381 gaccacccac aactcatttt tgaatctaca ggtatctatt caagaggtat ggaacgattt
     1441 tgttgtgtaa atcaaattaa ctatattcaa atgaatccgt tagaagccaa atttaaaacg
     1501 agcgctctaa gatcatggaa aactgatcag gcagatgctc ataagcttgc ttgtttagga
     1561 ccgacgctca aacaaacagg cagcttacct atacatgagt taatattctt tgaattaaga
     1621 gaacgtgccc gttttcatct agaaatcgag aatgaacaaa atcgacttaa atttcagatt
     1681 cttgaattac tccatcaaac attccctggt ttagaaagat tatttagtag tcgatattca
     1741 atcattgcac tcaacatcgc agaaattttt actcatccag acgtggttct tgatatcgac
     1801 aaggatgtac ttattacaca tatattcaat tctacagata agggaatgtc aatggataaa
     1861 gctacaaaat atgcacttca attaagagtg attgctcaag aaagctatcc taatgtcgat
     1921 agacattcct ttctagtcga aaaattacgc ttacttattc aacaattaaa acaatctatt
     1981 catcatctca aacaattaga tgatgccatg attcaattag cacaacaact cgattatttt
     2041 gaaaatattc attcgatacc tggtattggt aagctaagca cagctatgat tattggggag
     2101 attggtgata ttaagcgatt taaatcaaat aaacaactca acgcttttgt aggcattgat
     2161 atcaaacgat atcaatcagg tcatacacac tgtagagata ccatcaacaa gcgtggtaat
     2221 aaaaaagcga gaaaactttt attttgggtg attatgaata taataagagg gcagcatcat
     2281 tatgacaatc atgtcgtcga ttattactac aaactaagaa agcagcctaa tgagaaacct
     2341 cataagactg ccatcattgc ttgtataaat cgattattaa aaacgattca ttatctggta
     2401 atgaatcata aattgtacga ttatcaaatg tcaccacatt agccaaacgt acaattaaat
     2461 atattttaat accttattca aaaaattaaa atgaacggtt tagttaagta atgcttattt
     2521 taattataag tacttgacta atcgtaagaa agagcctagg acataaatca atgtctcgcg
     2581 accacagctt aatttttggt gttcattcat gactttaaaa aatccttatt gcataaatgt
     2641 acatagtgta gtactattca aaacgtaatt attacgattt gaattaagcg aggagaatga
     2701 aatgactaag acttatgacg tttggtggca aaaaggtcaa gaatcagatg atgatatggc
     2761 acgagaccat caagaagctt gggagagaac aataaaaatg cttgatacat ctgacatcga
     2821 agggaaaacg attttagatg tgggatgtaa tcaaggcgga tttttacgac agttatacga
     2881 tacaacaccg tttaaaaaag gtgttggcat agatttagca cgtttatctt tggaaaaggc
     2941 agagacatta aaaggacaac gtccacttac atactattta acagataaac cgcaagaaac
     3001 gaagcacgtg tttgatacgg cagtaagtac gtctgtcttg tacttaatag aagatattcc
     3061 gcaacatgca aaagatttaa aagaggtatt gaaaccaggc ggtgtttatt acgcttcatt
     3121 cgcggattta actaataacc caagtcgtca gtttatggat gacacgatta atcaatatgg
     3181 tgcaacacct tctcagaatc actctctaaa acatatcgtt gatagctttg tggatgcagg
     3241 atttgaagtt gcagtaatga aagagcatgt acctgacgtg attgatttaa cacattatag
     3301 cgatttttat ttatcaccga atgattattt acaaacacta tatgaagaat cgtttttaat
     3361 aaaagcaagt gtgaaagaag gtactgagaa atgaggaaat gtgtattaat gacggtagca
     3421 gcaagtgcta cgctcttatt ggcaggttgt ggcaatggtc aaaaagaaga taaagatgtt
     3481 acggtatcgc tacctactga agcaaaggcg gataaacttg acgcgcaagg ctatgatgca
     3541 gcgatgcccg tttatagtgc agtgtatgat gcattagtta aatatgataa agataagggt
     3601 attaaagcag gtttagcaga taaatggagc gttgatgaat cagggaaagt ttatgaattc
     3661 catttgaaaa agaatgttaa attctcagat ggttcagcat tagatgctaa ggacgtgaaa
     3721 ttctcgattg atcgtgcgaa agcgatgaac aaagattcga ctgtagaaac gttaaaaaaa
     3781 ttagataagg tcgttgttaa aaatgagcac gtggtccaaa ttagattgaa atctccttca
     3841 aatcaagtgt taaatgaatt aacacaagtg agaccgttgc gtattatgag tccacattca
     3901 gtagaagatg gtaaagtaaa cggtaaattt gaaaaagcga ttggaacagg tgcatttgtt
     3961 gttgataaaa ctggtaaaga aaaaacgaca atgaagccaa ataaatattt tgacaacggt
     4021 cacccagtca attatcatct tgcattccaa acgattgaag atggggactc aagaaattct
     4081 gcagtacaaa gtggttctgt agatatttct ggtggtgctt taggtatgct ctcagacgaa
     4141 caaatcaaac aagataagaa aaataagaac ttaacgattg aagatagacc tagcacagta
     4201 agtcacttta tggcatttaa ccctaaaaat gatgtattaa atcaacgcac aattcgtgaa
     4261 gcgataagta agagcatcga tgcgaaagac attgcgggca aatctgtaaa tggtctgttc
     4321 cagaagaacg tacaatttgt gactaaaaat aatcaacagc cacacgatta tgatatgaaa
     4381 gcggctgaaa ggttacttaa atcagaagga tatcataaaa acgatgacgg catctttgaa
     4441 aagaatggca aacctttatc atttaactta gtcattcaaa ctgcagagtt cccaaattgg
     4501 aaagataaag ctgaaaaagt gcaacgtcag cttaaacaag ccggtattaa gttaaatgtg
     4561 aaaacgttag attcacaatc atactatgat acattatgga cgaaaaaaga ctatgatttg
     4621 attttctata gaacgtattc agatgcatta atgccttaca actttataag ttcagtgttt
     4681 aaaaataatg atggtcaacc aggggtgtta gctgatgatg aaacattaac gaaacagcta
     4741 gacgatttcc catcaaccgt atcaaaagaa gaccaacagt gttcatttga tgacatattt
     4801 aaacacttta atcaacaata ctatggtgtg ccaattgctt atccaaatga gacgtttgta
     4861 gtgagtgata aagtaaaaca attcaaattc tctggactta cggatgcacc aattgattat
     4921 aaagcgttga aagttaatga atagcaatgc tcaaacgtac aattaaattc atactttatt
     4981 taatcgtaag ttcgtttatt atcttcattt tagttgagaa gacatctggt aatccagcga
     5041 ttctgtatct acaacgtcat ggttatacgt cgattacgca agacaatatt gaagcggcac
     5101 aacatcaact tggcttagga caacatgtgt tactaagata tatcgattgg gttggacatg
     5161 cactcacggg caacttagga tacggcttta gtacgaacga agcagttacc gctatgataa
     5221 tggaagccat cgtgccgacg cttgtgctaa tcattgtctc tagttgtatc atgttgccat
     5281 ttggctatat tgttggttac ttcgttggga cgcgtccgca tacacgttac gctaatggaa
     5341 ttcgtggatt cgcccaagtg atgacctcaa tgccagaata ctggttagct attttattca
     5401 tttattattt aggcgtacgt tggcaattgt taccatttgt aggtagtgat tcatggcaac
     5461 actttgtgct gccaatcttc acaattgttg ttatagaagg gtgtcatatc ttattgatga
     5521 cagcacatct gattacacaa acgttagatc aagatgcgta tcaactggcg cagttaagac
     5581 atttttcgtt aaaagcgcgt atcatcgtac aaattaaaga gatatttgca ccactaatga
     5641 cgatttcaat taacagtatc attcatttaa ttggaaaagc cgtaatacta gaagtcatct
     5701 tcagcatgtc tggtataggt aaattgttga ttaatgctat taaccaacga gattatccac
     5761 tgattcaggg cattgtcatc tttatcattg tctttattat gctaatgaat tatttaggcg
     5821 atgtgattat tttgaagaat gaacctagac ttcgacgacg tcatacccag cagtcaggca
     5881 atgagaaaag aggtacgatg tgatgaaaaa atatcaaacg tacatcgcaa taggttcact
     5941 attgagtttg atggttgtat taattacgta tggtttaatg caagacacgc aacatttgaa
     6001 cccacttgag tcacctaatg gacaacattg gttgggtacc gatcaattag gcagagactt
     6061 cttagtaaga ctgattgtcg gtagtcttgt cacattgagt ttaacaggca tagtgattct
     6121 attaagcgtt tgtatgggac ttatctttgg cttaattgca ggcatagaaa gacgatggtt
     6181 agatcaaatc atcatgtttg ttgccgatat gttgctggct attccgtcat ttattatcgc
     6241 attagtcatc ttaagtttag taagtaactc catgataggt ttgatacttg ctttaacgat
     6301 tggatggata ggacgttatt tacgttactt cagaaattta acgcgagata ttcaaaaacg
     6361 tccatttgtt caatatgcac gattgagtgg gaactcaaca ttcaaaacga cagtaacaca
     6421 tgtgattcca catttattaa gtagtatatt cgctttggta acggctgact ttggcaaaat
     6481 gatgctcagc atatctggac ttgcttttct aggactaggt attaaaccgc cgacgcctga
     6541 gttaggaaca attctttttg atgggaaaag ttatttcaac ggcgcaccgt ggctcttctt
     6601 cttccctggt gtattgttag gaggtttcgc cttattatgt caaattatca acaaaaaaat
     6661 aacgcagtaa atacggtagt caacgtcaat caattatcga ttttagatca agagaaagta
     6721 ttgttaaagg atgttgattt gacagtaact aaaggtgcat ttcattgcat tataggtgaa
     6781 agtggcagtg ggaaatcact gttaacaaga acaatacttg gaatgaaaca atcacaatta
     6841 tgttatcaag gagatattga catcgattta actcaaacag atgcagtgtt tcaagatgtt
     6901 caaagtaata tgtttcaaaa tataacatta gctaagcatt tccaatacat ttatgaagcc
     6961 aatcgcacac atctcactaa acagcgtatt aaggaagatg tcttacagat gatgcaatta
     7021 cttggtttaa gacaagggga acaattgctt gagcgttatc ccttcgaact tagtggaggt
     7081 atggcacaac gtgtcgcctt tataatgtca ttaattagac gtccgaacta cttattttta
     7141 gatgaaccaa cgagtgcact tgatcaagaa aatattaaaa agtttatgca ttaccttctt
     7201 agggcacagg agcgctacca aatgaccatt gtttttatca cacatgatat taacttagtg
     7261 aaagattgtg ccacacatat tagtattatg cagcaaggta aattgataga aaatggtgag
     7321 gccgcgtcga tcttaactaa gccgacacat aattacacga aaaaattaat tacgattgca
     7381 catcggagac aaccttatgc ttaaaataga gagattaacc aaatatatag acacgcaact
     7441 gatatttaaa gagatatcat gtacaattaa cgaccagcac ttactcataa gtggggagag
     7501 tggttgtggt aaatccacat tagccaagat tatcgctggc ttagatacgg attatcaggg
     7561 cgaattatat cttaatgggc gcttacgtga atcttatacg tctaaagagt ggatgaagca
     7621 catccaatat gtacctcaat atcaacgtga tactttaaat cagcgtaaaa cggtattagc
     7681 tacattatta gaaccactta agaattataa ggtaaataaa cagcgttata catcaagcat
     7741 tgaagcagtg cttgatcagt gtaatttacc acacgatata cttaatcata aagtttcgac
     7801 attaagtggt ggccaatttc aacgcgtctg gatagctaaa gctttaatat tagaaccaga
     7861 gattctcata ttggatgaag ctacaaccaa cttagatgtc attaatgaag aagctatact
     7921 tcaaatgttg atttccttaa agatgacaca attaatcatt atttcacatg atacatacgt
     7981 cttaagccaa tttgaaggaa ttcagttaca gctaaataaa ttgaataatt aagatcacaa
     8041 atcttaatat ggtgaatatt taatggtacc taaaaaataa aatttaaact acaatgtcta
     8101 aatccatatg ttgtttcatt agaggattta aaaatgatta taacactaaa agatttcaaa
     8161 ttattatatt taatataaat ttacatatga taaacgaata acaattccaa tataaattat
     8221 ttt
//
LOCUS       DALAGD010000115         8142 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.116, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000115 DALAGD010000000
VERSION     DALAGD010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8142
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.116"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(10..159)
                     /locus_tag="O2701_002400"
     CDS             complement(10..159)
                     /locus_tag="O2701_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294273.1"
                     /translation="MKIKTFATFTHRQLDKQVNRFLKNDIHVLEIQTHSNWLYISAIV
                     FYEDK"
     gene            complement(706..1022)
                     /locus_tag="O2701_002401"
                     /pseudo
     CDS             complement(706..1022)
                     /locus_tag="O2701_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002483623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
     gene            complement(1015..1998)
                     /locus_tag="O2701_002402"
     CDS             complement(1015..1998)
                     /locus_tag="O2701_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCZ4294274.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILR
                     DRLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGL
                     NKPNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRKSNVQKFENRTSRSSKIERPE
                     VRKSNTNDTDFNDIDFNDTENNDMNDLNDIKYKNEISNHSNHSNQFTHNFDDKEMLLQ
                     EFPEQLTNYLLKYDYRDLEIIKAVILKAKKSFNSRHEDMHYMLEDIEDEILTSLKRLK
                     KAIHDRGVKGQKETIKSMQAYLMQTILTELEETHALYMRRKNMKQYNIFNQ"
     gene            2424..3167
                     /locus_tag="O2701_002403"
     CDS             2424..3167
                     /locus_tag="O2701_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCZ4294275.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDI
                     EKIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEK
                     QESRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTST
                     NTRQNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPA
                     NLNDNDSDIEEKSEETEPKKGFWSRLFGS"
     gene            complement(3394..3579)
                     /locus_tag="O2701_002404"
     CDS             complement(3394..3579)
                     /locus_tag="O2701_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011276570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ4294276.1"
                     /translation="MKNRIREYRKQKGISQETLSKELKVSRQTINAIENNKYDPTLTL
                     AFKLARLFDTTVDELFS"
     gene            complement(3584..3922)
                     /locus_tag="O2701_002405"
     CDS             complement(3584..3922)
                     /locus_tag="O2701_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294277.1"
                     /translation="MNLIPFIIILIFSCLWTAITNKYLPSETLDKKGQQDRYDERQRK
                     MFIEILAKSFIWIVYCMLFTLVLKFTGLSPSIEGSWFSQYPEIFFIIVALFLMLFNYY
                     TTNKKYTSKG"
     gene            complement(4168..4776)
                     /locus_tag="O2701_002406"
     CDS             complement(4168..4776)
                     /locus_tag="O2701_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCZ4294278.1"
                     /translation="MIIGYARVSSIDQNLERQLDNLKTFGVEKIFTEKQSGKSVENRP
                     VFQEALNFVRMGDRFVVESIDRLGRNYDEIIETVNYLKEKDVQLMITSLPMMNEVIGN
                     PLLDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQ
                     KRVIYHRVVEMLEEGKAISKIAKEVNITRQTVYRIKHDKGLS"
     gene            complement(5262..6161)
                     /locus_tag="O2701_002407"
     CDS             complement(5262..6161)
                     /locus_tag="O2701_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(C) family macrolide 2'-phosphotransferase"
                     /protein_id="HCZ4294279.1"
                     /translation="MTRHNEIIKCAEKYQLHIQPQTISLNESGLDFQVAFGKDKHGVE
                     WVLRLPRRPDVYKRTKPEKQTVDFLQKNVSFEIPKWKVHAKDLIAYPKLTGKPAATID
                     PEIQNYVWEIEHKPLPENFINTLAETLVDLHNIPEENINVQHINIKTIQEIKNDFQRR
                     MNKVKETYGVSDELWNRWKQWLENDELWPRHATMIHGDLHPGHIMVDNQANVTGLIDW
                     TEATHSDPSMDFIGHHRVFDDEGLEQLITAYGKAGGEIWPRMKEHIIELNAVFPMFIA
                     EFAMESGESAYETMALKELGMKE"
     gene            complement(6260..7726)
                     /gene="msr(A)"
                     /locus_tag="O2701_002408"
     CDS             complement(6260..7726)
                     /gene="msr(A)"
                     /locus_tag="O2701_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(A)"
                     /protein_id="HCZ4294280.1"
                     /translation="MEQYTIKFNQINHKLTDLRSLNIGHLYAYQFEKIALIGGNGTGK
                     TTLLNMIAQKTKPESGTVETVGEIQYFEQLNMDVENDFNTLDGSLMSELHIPMHTTDS
                     MSGGEKAKYKLANVISNYSPILLLDEPTNHLDKIGKDYLKNILKYYYGTLIIVSHDRA
                     LIDQIADTIWDIQEDGTIRVFKGNYTQYQNQYEQEQLEQQRQYEQYISEKQRLSQASK
                     AKRNQAQQMAQASSKQKNKSIAPDRLSASKQKGTVEKAAQKQAKHIEKRMEHLEEVEK
                     PQSYHEFNFPQNKIYDIHNNYPIIAQNLTLVKGSQKLLTQVRFQIPYGKNIALVGANG
                     VGKTTLLEAIYHQIEGIDCSPKVQMAYYRQLAYEDMRDVSLLQYLMDETDSSESFSRA
                     ILNNLGLNEALDRSCNVLSGGERTKLSLAVLFSTKANMLILDEPTNFLDIKTLEALEM
                     FMNKYPGIILFTSHDTRFVKHVSDKKWELTGQSLHDIT"
BASE COUNT         2638 a         1260 c         1134 g         3110 t
ORIGIN      
        1 cgatgttcct tatttatcct cataaaaaac aatagctgaa atatataacc aattactgtg
       61 tgtttgaatt tctaatacat gaatatcatt ctttaaaaac ctattaactt gtttatctaa
      121 ttgacgatga gtaaaagtag cgaaagtttt aatcttcatg atatctctcc ttttataaaa
      181 atagcaatcc ttattacaat attaaacact tttattttgg attttaagag gcattttgaa
      241 gacttagagg caaatcatat aggttttgta cttaaaaatc tatacagtct cttaaaacgc
      301 aaatatgagc caaataaata tatgctaatt tcacaaacaa aaatttcagc aaaatcagtg
      361 actaattttt tagatactgc ccatttacat gcaaatttaa aaattagcat aaaatacttt
      421 aaaacttccc catgtttttc taaattcctg cagggctttt cttatcaaaa acttagaaat
      481 tttaatttaa ctctaaaatc tccgtaagag cataagggaa atttgctatc attcaaaaat
      541 gatagatctg gcaaggccag acattccaag ggttttaagt tattttaaag tgatatcata
      601 caaagctgta gtgtcacact tttgtgacac tacatacagc atttgtcaca aaaataagac
      661 agcacttttt tacagaaaaa caataaaaaa agacatcagt tatcactaat gtcttaaata
      721 ataattttgc ttttcgaaat tccaaattgc ggtaattgaa tgtgcttttt ttcttcgtct
      781 gtcgttttat cttcatcact tgtattaatc aaattaccat cttcggcatc gtctaagttt
      841 aaaattttat gcttttgttt aagcaatcca ccataactta acatacgttt tcgatacaaa
      901 cctttttcta aatcacttac aatttcttga tttttttcgt cgtcatcagt taaaaagtca
      961 gatgatttaa cggaatattt tgatgtctct tcgagtgtta taaaatcatt tgaattattg
     1021 attaaagata ttatattgtt tcatgttttt acggcgcata taaagggcat gtgtttcttc
     1081 aagttcagtt agaattgttt gcattaaata agcttgcatt gacttgattg tttccttttg
     1141 gcctttaact cccctatcat gtatagcttt tttaagtctt tttaaagaag ttaatatttc
     1201 atcctcaatg tcctctaaca tgtaatgcat atcttcatgc cttgaattaa atgatttttt
     1261 tgctttaaga ataacagcct ttatgatttc taaatctcta taatcatatt ttaataaata
     1321 attagttagt tgttctggga attcttgcaa tagcatttct ttatcatcaa aattatgtgt
     1381 aaattgattt gaatgatttg aatgattact aatttcattt ttgtacttta tatcattcaa
     1441 gtcattcata tcattattct cagtatcatt aaagtcaatg tcattaaagt cagtatcatt
     1501 agtgttcgat tttcgaactt ctggacgttc gattttcgaa cttctggacg ttcgattttc
     1561 gaacttctgg acgttcgatt ttcgaacttc cttctcgccg taaggttcaa catcgttttc
     1621 ttctttttct attttgtaaa tatcacgttc tgtgacaata ggtttcatta aatatagaat
     1681 atttggttta tttaaaccac gtcgtttttg aatgagtaga ccatcgtttt ctaacccttt
     1741 ttttattttt gttaactttt cttttttaca atttaagatt tccattagct tttcatttga
     1801 ataaacaaag taaatgttac catcttcatc aacccagcta tttttaatag ataaatttaa
     1861 gcgatcacgt aagatagcat aggctatttt catatcatta gttagatttt tataattttc
     1921 actggtaaag aatactttag ggatttgata aaatctttct ttgtaatttt cttgcactgt
     1981 aaaatattga ttttgcatga caaaaaacct ctttccatat tttagtttgg taagaggtaa
     2041 taaaacgttt ttatttagct ttacatttat ttaatgattt aggtgcttgc actaagatat
     2101 tattcattat ataatacaag tatcgaaaat tatataaata aacagtttct aaacgtttta
     2161 ttaccaaacc ccgactatta ccagtagttg gggtttttgt tatattaagt ttattatagc
     2221 actttaaata aacttataaa agggaaaata tagctttatc aagggtttat aaacccttga
     2281 taaatatcat aaacctttca taaacccttg ataaaaaggt tgatggatgt ttatttttat
     2341 ttattaatca ctttattatt aagcattata agcttataat attttatcaa gggtttataa
     2401 acattttata aagggttgat gatatgaaaa gtgtgaaaaa gttgagtgaa gaattagggg
     2461 ttagtaagca aacgattttt aataatatta agcgattgaa tatagagaca ataaaacagg
     2521 aaaacacttc gtttattaaa gaagataccg atatagaaaa aatcattcaa agagtaaatg
     2581 aaaataaaaa gaaatatggt tttgaaagta ctactgaaga caaacaaaaa aaagggtcgg
     2641 ataatattaa ttttgaatcg aaaagcgatg ctcaaatagt agaaatcctt aaaaatcaga
     2701 ttgatacttt aaataatcaa attgaaaaac aagaaagtcg tcatgaaaca acaattgaat
     2761 tttacagaaa agagttgcaa gaaagatcaa aattgcttga aaatcagcaa gttctggcat
     2821 tagagagtaa taaaaagatt caaaaattag aaaatcaatt agaaaaagaa aaacacctta
     2881 attatccctt tgatacatct actaacacta gacaaaacgt tgatgcacaa gaaaaaacat
     2941 atactactaa tcctgtaaat attaacagag atcaggaaga aactaaagaa acagagattc
     3001 aatataagga tatatctgga agtcaaagcg acgaaagtac acaaggtgaa gaagcaaaaa
     3061 aagaagacgt tccagctaat ctaaatgaca atgatagtga tatagaagaa aagagtgaag
     3121 aaacggaacc taaaaagggg ttttggagtc gtttgtttgg tagctaaaat aattgattag
     3181 gcactaaaag ttataaaatt ttagtgtctt ttatatagtg agaacttctg gtaatcatcg
     3241 attatgtaaa ctaaagtatt tcaaataatt aactgtatta atactctcat catgattgcg
     3301 ttataaacgt tcgttattga gacaaaattt aatagtacta ctacacaaaa agagagtgcg
     3361 aattcgcact ctctttttgt gataaatgaa tggttaagaa aataattcat ccacagttgt
     3421 atcaaacaat cttgccaact taaatgctaa tgtcaatgta ggatcatatt tattgttttc
     3481 tattgcatta atagtttgcc tagacacttt taattcttta cttaaagttt cttgagaaat
     3541 tcctttttgt tttctatatt ctctaattct atttttcatt aatttaacct ttagatgtat
     3601 attttttatt tgtagtatag taattaaaca gcataagaaa tagtgctaca attataaaga
     3661 aaatttctgg atattgagaa aaccaagatc cttcaatact tggtgacaaa cctgtaaatt
     3721 taagcactag tgtaaataac atacaatata caatccaaat aaaactttta gccagtattt
     3781 caataaacat tttacgttgt ctttcatcgt atcgatcttg ttgtcctttt ttatctaatg
     3841 tttctgaagg taaatatttg ttagttatag ccgtccataa acaagaaaaa attaaaataa
     3901 taataaaagg aattaaattc attataacac ctcataaatg taaaaagtat ttgacattta
     3961 tgagtgtaaa tatttttgat acaaatgtca aatacttttt acattataat atattttaaa
     4021 acaccttatt agtaaaacaa ttactatata gaaagctgcg aactcgtact cttttttctt
     4081 gtataattat taaaatctca attcaataat atgttatgga atttgagaca ctgtgagaga
     4141 cgctcatttg cattgacaat caatttatca agataatccc ttatcatgtt taattctata
     4201 aactgtttgt cttgtgatat taacttcttt cgcaatctta ctaatcgctt tgccttcttc
     4261 taacatttcc accacgcgat gataaataac acgtttctgt gggtctttag catttggtga
     4321 ataaagtaaa gggcgccctt tatatacgcc tttttccttc gcaacttgaa tcccttgcgc
     4381 ttgtcgacgt ttactttcat ttctctcttg ttctgaaacc attgctaata tttgaatgat
     4441 taaatctttc ataaacttat ccagtaatgg attgccaatt acttcattca tcataggtaa
     4501 gctggtaatc attaactgaa cgtctttttc ttttaaataa ttcacagttt caataatttc
     4561 atcatagtta cgtcctaaac gatcaataga ttccacaacg aatcgatctc ccattctgac
     4621 aaaattgagt gcttcttgaa atacaggtct attttccact gactttcccg actgtttctc
     4681 tgtaaagatt ttctccacac caaacgtctt taaattatcc aattgtcttt ctaaattttg
     4741 atctatcgat gatactctcg cataacctat aatcattcat cttcactcca taataaatat
     4801 acaccctaat catacgtttt aattgttata atttcaacaa ttctttttgt atggttaggg
     4861 tgtactcaaa gtgtacatat ctataattaa aacttactaa aaataaatat agattttttc
     4921 tttgtaacag aaccgttcat ttgaaggtca tctctatcat aaatatttaa attaactaaa
     4981 aattttaatc atctgtttaa tacttatgaa atgtatttac ggtaggtatt aaaattgatg
     5041 aaatttatat aagtgctttc aatgaaaatg accgtttatt tatcctcctg atttaaggag
     5101 ttagaaattt cgaaaacgat tctttccatt tgttgcattt gttccatgtc cagatattta
     5161 atacattata gaaaaccaaa gaccatcact aattaaacgc agttttaaaa tttcatcagg
     5221 attgccttcg tctttaaaat gggcctcttt taatataagt tctactcttt catacctaac
     5281 tctttcaatg ccatcgtttc atacgccgat tctcctgatt ccatagcaaa ctcagcgata
     5341 aacattggga atactgcatt gagttctatt atatgctctt tcattcgtgg ccatatttca
     5401 cctccagctt taccgtatgc tgttataagt tgctctaatc cttcgtcgtc gaatacacga
     5461 tggtgtccta taaagtccat tgatgggtcg gagtgggttg cttcagtcca gtctatgaga
     5521 cctgtgacgt ttgcttggtt atctaccatt atatgtcctg gatgtaagtc cccatgtatc
     5581 atggtcgcat gtcgaggcca tagttcgtcg ttttctaacc attgtttcca tctgttccat
     5641 aattcatctg atacgccata agtttcttta actttattca ttcttctttg aaagtcattt
     5701 tttatttctt gtatggtttt gatatttata tgctgaacgt taatgttttc ttctggtatg
     5761 ttgtgtaaat ctacgagtgt ttcagctaat gtgttaataa agttttctgg taatggtttg
     5821 tgttcaattt cccatacata attttgtatt tctggatcta ttgtggctgc gggtttacct
     5881 gtaagttttg ggtaagcaat aaggtctttt gcatgtactt tccacttcgg tatttcaaat
     5941 gaaacattct tctgtaagaa gtctaccgtt tgtttttcgg gttttgttcg tttataaacg
     6001 tcaggtcttc ttggtagtct caaaacccat tctactccat gtttatcttt tccaaatgca
     6061 acttgaaaat caagtcccga ttcattcaat gagattgttt gaggttggat gtgtaattga
     6121 tatttttctg cacatttaat aatttcatta tgtcgagtca ttttattgga tccttatttc
     6181 tattatattt ttcttgtgaa cgtatttttt atattgatat aaatgaacct aatattcaat
     6241 ataaattagt gtatagtttt taagttatat catgaagaga ttgtcctgtt aattcccatt
     6301 ttttatctga tacatgttta acaaaccttg tatcatgtga tgtaaacaaa atgattccag
     6361 gatatttatt cataaacatt tctaatgctt ctaatgtttt aatatctaaa aaattagttg
     6421 gttcatccaa aattaacata ttcgctttcg ttgaaaataa tactgctaac gataatttcg
     6481 ttctttcccc accactcaaa acattacaag aacgatcaag tgcttcattt aaacccaagt
     6541 tatttaaaat agctctactg aatgattctg atgaatccgt ttcatccatt aaatattgca
     6601 ataatgaaac gtcacgcatg tcttcataag caagttgacg atagtatgcc atttgcactt
     6661 taggagaaca atcaattccc tctatttggt ggtaaatagc ttcaagtaaa gttgtcttac
     6721 ctacaccatt tgcacctacg agcgctatat ttttgccata tggtatttgg aatcgtactt
     6781 gtgttagcag tttttgactt cctttaacca atgttagatt ttgtgcaatg attggataat
     6841 tattatggat atcataaatt ttattttgtg ggaaattgaa ttcatgataa ctttgtggtt
     6901 tttcaacttc ttccaaatgt tccattcttt tttcaatatg cttagcttgt ttttgagcag
     6961 ccttctcaac cgtgcctttt tgttttgatg cacttaaacg atctggtgct atacttttgt
     7021 ttttttgttt tgatgatgct tgtgccattt gttgcgcttg atttcgttta gctttactgg
     7081 cttgggacaa tctttgtttt tcacttatat actgttcata ttgacgttgt tgttctaact
     7141 gttcttgttc atattgattt tgatactgtg tgtaattacc tttaaacact cttattgtgc
     7201 catcttcttg tatatcccaa attgtgtcag caatttggtc tataagtgct ctatcatgac
     7261 ttactataat taaagtacca tagtaatatt ttaaaatatt tttcagataa tctttaccaa
     7321 ttttatccaa gtgatttgta ggttcatcta aaagtaatat cggactataa tttgatatga
     7381 cattagctaa tttatatttt gctttttcac caccactcat actgtcggtt gtatgcatag
     7441 gtatatggag ttcactcatt aaactaccgt ctaacgtgtt aaaatcattt tccacatcca
     7501 tgttaagctg ttcaaaatat tgaatttcgc caaccgtttc aactgttcca gattctggtt
     7561 ttgttttttg agcaatcata tttagtaatg tggttttgcc agtaccatta cccccaataa
     7621 gtgctatttt ttcaaattgg taagcgtaaa gatgaccgat gttaagtgat cgtaaatctg
     7681 tcaatttatg attgatttgg ttaaatttaa ttgtatattg ttccatattt atacactcct
     7741 tgtatattta aaggacgtgt acgcaaaaaa atgagacagg ctactcccgt ctcattcaca
     7801 ttacactttc aaacacttaa aaatttatta tgatgtttta gtatttgaaa gcgtttatta
     7861 ttcctatcga aggataaaaa atgacataag tcatcctatg agtgtgtatt gcggacaagc
     7921 ctttttgaat taatttagtt ttgtattcaa aaaggtttat ttgagtctca tagaagctgt
     7981 cattttaatc ctccttcatc gataaaatag taatgttaat ataacatttt gttggttttt
     8041 ttacaatata tctctaagta gaaagattat ataacaaaag tataaaaata caattttaga
     8101 ttgattatac atctccttaa tttagagtgt actcataaaa ta
//
LOCUS       DALAGD010000116         8088 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.117, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000116 DALAGD010000000
VERSION     DALAGD010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8088
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.117"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            54..845
                     /gene="pgeF"
                     /locus_tag="O2701_002409"
     CDS             54..845
                     /gene="pgeF"
                     /locus_tag="O2701_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HCZ4294281.1"
                     /translation="MNDNFKKQPHHLIYEELLQQGITLGITTRGDGLSDYPKNAFNMA
                     RYIDDRPYNITQHQLQLAEEIAFDRKNWVFPIQTHENKVACITKDDIGTNIDTLTDAL
                     HGIDAMYTYDSNVLLTMCYADCVPVYFYSTKHHFIALAHAGWRGTYTEIVKEVLKHVN
                     FDLKDLHVVIGPSTSSSYEINDDIKNKFETLPIDSANYIETRGRDRHGIDLKKANAAL
                     LIYYGVPKENIYTTAYATSEHLELFFSYRLEKGQTGRMLAFIGQQ"
     gene            869..1537
                     /locus_tag="O2701_002410"
     CDS             869..1537
                     /locus_tag="O2701_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HCZ4294282.1"
                     /translation="MKDNLQQISTQINDKSEKNNFSTKPNVIAVTKYVTIERAKEAYE
                     TGIRHFGENRLEGFFQKKEALPSDAVIHFIGSLQSRKVKDVINDVDYFHALDRLSLAK
                     EINKRAEHKIKCFLQVNVSGEASKHGIALEDVDQFIDDLKKYDKIEIVGLMTMAPLTD
                     DEAYIRSLFKQLRLKKEEIQRLNLEYAPCDELSMGMSNDYLIAVEEGATFVRIGTKLV
                     GEEE"
     gene            1534..2097
                     /locus_tag="O2701_002411"
     CDS             1534..2097
                     /locus_tag="O2701_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HCZ4294283.1"
                     /translation="MSHLALKDLFSGFFVIDDEEEVEVPDKQQQVNEAPAKEQSQQTT
                     KQNAIKSVPQKSASRYTTTSEERNNRMSNYSKNNSRNVVTMNNATPNNASQESSKMCL
                     FEPRVFSDTQDIADELKNRRATLVNLQRIDKVSAKRIIDFLSGTVYAIGGDIQRVGTD
                     IFLCTPDNVEVAGSITDHIENMEHSFD"
     gene            2109..2399
                     /locus_tag="O2701_002412"
     CDS             2109..2399
                     /locus_tag="O2701_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HCZ4294284.1"
                     /translation="MDINVLATIFKFILFVVEIYYFGMIIYFFTSWVPSIRETKVGYF
                     LAKIYEPFLQPFRKVIPPIGIIDISSIAAIIVLVLFQKGLLQIFNWILIQLQ"
     gene            2509..3288
                     /locus_tag="O2701_002413"
     CDS             2509..3288
                     /locus_tag="O2701_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCZ4294285.1"
                     /translation="MINIDIYQHFRQEEYELIDQLTDKCDQAEQHYAPVLTHFLDPRG
                     QYILEVICGSYEDLNVSFYGGPNAERKRAIISPNYYEPKESDFELTLMEIDYPEKFVT
                     LKHQHILGTLMSLGIEREQVGDIIVNERIQFVLTSRLESFIMLELQRIKGASVKLYTI
                     PVTDMIQSNENWKNESATVSSLRLDVVIKEMIRKSRTIAKQLIEKKRVKVNHTIVDSA
                     DFQLQANDLISIQGFGRAHITDLGGKTKKDKTHITYRTLFK"
     gene            3312..3929
                     /locus_tag="O2701_002414"
     CDS             3312..3929
                     /locus_tag="O2701_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HCZ4294286.1"
                     /translation="MPFTPNEIKNKEFSRVKNGLEPTEVANFLEQLSTEIERLKEDKK
                     QLEKVIEERDTNIKSYQDVHQSVSDALIQAQKAGEETKQAAEKQAEAIIAKAEAQANQ
                     MVGDAVEKARRLAFQTEDMKRQSKVFRSRFRMLVEAQLDLLKNEDWDYLLNYDLDAEQ
                     VTLENIHHLHENDLKPDEVAANAQNNASNTPDNNQQSNDSETTKK"
     gene            4150..6903
                     /gene="ileS"
                     /locus_tag="O2701_002415"
     CDS             4150..6903
                     /gene="ileS"
                     /locus_tag="O2701_002415"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCZ4294287.1"
                     /translation="MDYKETLLMPKTDFPMRGGLPNKEPQIQEKWDAEDQYHKALEKN
                     KGNETFILHDGPPYANGNLHMGHALNKILKDFIVRYKTMQGFYAPYVPGWDTHGLPIE
                     QALTKKGVDRKKMSTAEFREKCKEFALEQIELQKKDFRRLGVRGDFNDPYITLKPEYE
                     AAQIRIFGEMADKGLIYKGKKPVYWSPSSESSLAEAEIEYHDKRSASIYVAFDVKDDK
                     GVVDADAKFIIWTTTPWTIPSNVAITVHPELKYGQYNVNGEKYIIAEALSDAVAEALD
                     WDKASIKLEKEYTGKELEYVVAQHPFLDRESLVINGDHVTTDAGTGCVHTAPGHGEDD
                     YIVGQKYELPVISPIDDKGVFTEEGGQFEGMFYDKANKAVTDLLTEKGALLKLDFITH
                     SYPHDWRTKKPVIFRATPQWFASISKVRQDILDAIENTNFKVNWGKTRIYNMVRDRGE
                     WVISRQRVWGVPLPVFYAENGEIIMTKETVNHVADLFAEHGSNIWFEREAKDLLPEGF
                     THPGSPNGTFTKETDIMDVWFDSGSSHRGVLETRPELSFPADMYLEGSDQYRGWFNSS
                     ITTSVATRGVSPYKFLLSHGFVMDGEGKKMSKSLGNVIVPDQVVKQKGADIARLWVSS
                     TDYLADVRISDEILKQTSDVYRKIRNTLRFMLGNINDFNPDTDSIPESELLEVDRYLL
                     NRLREFTASTINNYENFDYLNIYQEVQNFINVELSNFYLDYGKDILYIEQRDSHIRRS
                     MQTVLYQILVDMTKLLAPILVHTAEEVWSHTPHVKEESVHLADMPKVVEVDQALLDKW
                     RTFMNLRDDVNRALETARNEKVIGKSLEAKVTIASNDKFNASEFLTSFDALHQLFIVS
                     QVKVVDKLDDQATAYEHGDIVIEHADGEKCERCWNYSEDLGAVDELTHLCPRCQQVVK
                     SLV"
     gene            7193..7990
                     /locus_tag="O2701_002416"
     CDS             7193..7990
                     /locus_tag="O2701_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCZ4294288.1"
                     /translation="MYHNSNANFVNGITLNVRDKNELKPFYEDILGLNIINETLTSIQ
                     YEVGQNNHVITLVELQNGREPLMSEAGLFHIAIKLPQISDLANLLIHLSEYDIPVNGG
                     IQPASLSLFFEDPEGNGFKFYVDKDEAQWTRQNNLVKIDIRPLNVPRLVSHATKLLWL
                     GIPDDAIIGALHIKTIHLSEVKEYYLDYFGLEQSAYMDDYSIFLASNGYYQHLAMNDW
                     VSATKRVENFDTYGLAIVDFHYPETTHLNLQGPDGIYYRFNHIEVED"
BASE COUNT         2956 a         1139 c         1536 g         2457 t
ORIGIN      
        1 aactttgtac agttagacga agataattta aatgaaatga tggtgacgat cgagtgaatg
       61 ataattttaa aaagcaaccg catcatttaa tatatgaaga gttattacaa caaggtatta
      121 ctctaggtat tacaactaga ggagatggtt taagtgacta tcctaaaaat gcttttaata
      181 tggcgagata tattgatgat cgcccatata atattactca acatcaattg caattagctg
      241 aagaaattgc gtttgataga aaaaattggg tgtttcccat tcaaacacat gaaaataaag
      301 tcgcttgtat tacaaaggat gatataggca caaatataga cactttaact gatgcgcttc
      361 atggtattga tgcgatgtac acatatgata gtaatgtctt attaacgatg tgttatgcag
      421 actgtgtacc agtatatttt tatagtacaa aacatcattt tattgcattg gcgcatgcag
      481 gttggcgtgg tacctatact gaaattgtaa aagaagtgct aaaacatgtg aactttgatt
      541 tgaaagactt acatgtcgtt attggaccat ctacatcatc aagttatgaa attaatgatg
      601 atattaaaaa taaatttgaa acattgccaa ttgatagtgc caactatatt gaaactagag
      661 gacgagatcg tcatggtatt gatttgaaaa aagccaatgc tgcattatta atttattatg
      721 gtgttcctaa agaaaatatt tatacgacag cgtatgctac atctgaacat ttagaattat
      781 ttttctctta tcgattagaa aaaggtcaaa caggacgcat gttagcattc attggtcaac
      841 agtaaacaag gaggagatat gtttgcgtgt gaaagataat ttacaacaaa tctcaacaca
      901 aattaatgac aaaagtgaaa aaaataattt ttcaacaaaa ccaaacgtga ttgcagttac
      961 aaaatatgtt acaatagagc gagctaaaga agcgtatgag actggaataa gacattttgg
     1021 tgagaataga ttggaaggct tttttcaaaa gaaagaagca ttaccatcag atgcggtgat
     1081 ccattttata ggatcattac aatctcgaaa agttaaggac gttataaacg acgtagatta
     1141 tttccatgct ttagatcgat tgagcttagc caaagaaatt aacaaacgtg cagaacataa
     1201 aattaaatgt ttcttgcaag tgaacgtttc gggagaagct tctaaacatg gtattgcttt
     1261 agaagatgtt gatcagttta tagatgatct taaaaaatat gacaaaatcg aaattgtagg
     1321 tttaatgacg atggcaccat tgacagatga tgaagcatat attagatcgt tatttaaaca
     1381 gttacgtttg aaaaaagaag aaatacaacg actcaattta gaatatgcgc cttgtgatga
     1441 attatcaatg ggaatgagta atgactatct tattgcagtt gaagaaggtg cgacgtttgt
     1501 tagaattggg actaaacttg taggagaaga ggagtgagcc acttggcttt aaaagattta
     1561 tttagtggat tttttgtaat agatgatgaa gaggaagtag aagtacctga caaacaacaa
     1621 caggtaaatg aagcgccagc aaaagagcag tcacaacaaa caacaaaaca aaacgcaatc
     1681 aaatcagtcc ctcaaaaatc tgcatcaaga tatacaacaa cgtcagaaga aaggaataac
     1741 cgtatgtcta attattcaaa aaataattca cgtaatgttg taactatgaa caatgctaca
     1801 ccaaacaatg catcacaaga aagttcaaaa atgtgtttat tcgaaccacg tgttttttca
     1861 gatacacaag atattgctga tgagcttaaa aaccgccgtg cgacacttgt caatttacaa
     1921 cgtattgata aagtatcagc gaaaagaatt attgattttt taagcggtac tgtttatgca
     1981 atcggtggag atatccaacg tgtaggtact gatattttct tatgtacgcc tgataatgtg
     2041 gaagtagctg gaagcattac agaccatatt gaaaatatgg aacattcatt cgactaagga
     2101 gttaacatat ggatataaat gtgctagcta caatatttaa attcatcctt tttgttgttg
     2161 aaatttatta tttcggcatg attatatatt tctttacatc ttgggtacca agtattagag
     2221 aaactaaggt aggttatttt ttagcgaaaa tatatgaacc tttcttacaa ccatttagaa
     2281 aagtaattcc acctattgga attatcgaca tatcatcaat cgctgcaatt atcgttttag
     2341 tattattcca aaaagggtta ctccaaatct ttaattggat tttaattcaa ttacaataaa
     2401 ttaagcgaca taacgaggca agggtacatg atactttagc ctcgtttttg atatgcattt
     2461 ttctgaatat aagggcaata gatggtattt tataattttt ttaaggtagt gattaacata
     2521 gatatttatc aacactttag acaggaagaa tacgaattaa ttgatcagct aacggataaa
     2581 tgtgatcaag cggaacagca ttatgcacca gtattaacgc attttttaga tccaagaggg
     2641 caatatatat tggaagtgat ttgtggcagt tatgaagatt taaacgtatc tttttatggt
     2701 ggacctaatg ctgaaagaaa aagagcaatc atttcgccga actattatga acctaaagaa
     2761 agcgactttg aattaacttt aatggaaata gattatcctg aaaaattcgt cactttaaaa
     2821 catcaacata ttttagggac attaatgtct ttaggtatcg aacgcgaaca agttggagat
     2881 ataattgtga atgaacgaat tcaatttgtt ttgacaagta gattggaatc atttattatg
     2941 ttagaattac aacgtattaa aggcgcatca gttaaacttt atactattcc agtaacagat
     3001 atgatacaat ctaatgagaa ttggaaaaat gaaagtgcaa cagttagttc tttaaggtta
     3061 gatgttgtta ttaaagaaat gatacgtaaa tcacgtacga ttgcgaaaca actaatcgaa
     3121 aaaaaacgtg ttaaagtgaa tcacactatt gttgattcag cagattttca attacaagca
     3181 aatgatttaa tatccatcca aggttttggt agagcacaca ttactgactt aggtggtaaa
     3241 actaaaaaag ataaaacgca cattacctat agaacattat tcaaatagta atgatttaag
     3301 gaggataaca aatgcctttt acaccaaatg aaattaagaa taaagagttt tcacgtgtaa
     3361 agaatggttt agaacctact gaagttgcta attttttgga gcaactaagc actgaaattg
     3421 aacgtcttaa agaagataaa aaacaacttg aaaaagtaat cgaagagaga gatactaata
     3481 ttaagtctta tcaagacgtg catcaatctg taagtgatgc tttgatacaa gctcaaaaag
     3541 ctggtgaaga aactaagcaa gctgcagaga aacaagctga agcgattata gctaaggcag
     3601 aagcgcaagc taatcaaatg gttggtgacg cggtagaaaa agcacgccgt ttagcattcc
     3661 agactgaaga tatgaaacgt caatcaaaag tatttagatc gcgtttccgt atgttagttg
     3721 aagcgcaatt agacttatta aaaaacgaag attgggatta cttgttgaat tatgatttag
     3781 acgctgaaca agtgacgctt gaaaatattc atcatttgca tgaaaatgat ttaaagccag
     3841 atgaagttgc agcaaatgca caaaataatg catcaaatac accagacaat aatcaacaat
     3901 ccaatgattc agaaacaact aagaagtaag aattaaataa agacagacgc gtaatataca
     3961 tttaactttt cacagcgaat taggtaatgg tgagagccta gtaaaagcat gtatgttata
     4021 tcactggctt tttaatattt aaataatgta atgagagaac tctaagttga gttaataagg
     4081 gtggtaccgc gagcaatcgt cccttttaat ttaacttaga gttttttaaa tttttaagga
     4141 gtgaaaaaaa tggattacaa agaaacgtta ttaatgccta aaacagattt cccaatgcga
     4201 ggtggtttac caaacaagga accgcaaatt caagaaaaat gggatgcaga agatcaatac
     4261 cataaagcgt tagaaaaaaa taaaggtaac gaaacattca ttttacatga tggcccacca
     4321 tacgcgaatg gtaacttaca tatgggacat gccttgaaca aaattttaaa agactttatt
     4381 gtacgttata aaactatgca agggttctat gcaccatacg taccaggttg ggatacacat
     4441 ggtttaccaa ttgaacaagc attaacgaaa aaaggtgttg accgaaagaa aatgtcaaca
     4501 gctgaattcc gtgagaaatg taaagaattt gctttagaac aaattgaatt acagaaaaaa
     4561 gattttagac gtttaggtgt tcgtggtgac tttaatgatc catatattac attaaaacct
     4621 gaatacgaag ctgcacaaat tcgtattttt ggagaaatgg cagataaagg tttaatttat
     4681 aaaggtaaaa agccagttta ttggtctcct tcaagtgagt cttcattagc agaagcagaa
     4741 attgaatatc acgataaacg ttcagcatca atttacgttg catttgacgt taaagatgac
     4801 aaaggtgtcg ttgatgcaga tgctaaattt attatctgga caacaacgcc atggacaatt
     4861 ccatcaaatg ttgcgattac cgttcatcct gaattaaaat atggtcaata caatgtaaat
     4921 ggcgaaaaat atattattgc agaagccttg tctgacgctg tagcagaagc actggattgg
     4981 gataaagcat caatcaaatt agaaaaagaa tacacaggta aagaattaga gtatgttgta
     5041 gcacaacatc cattcttaga cagagaatcg ttagtgatta atggtgatca tgttactaca
     5101 gatgctggta caggttgtgt acatacagca ccaggtcacg gggaagatga ctatattgtt
     5161 ggtcaaaaat atgaattgcc agtaattagt ccaatcgatg ataaaggtgt atttactgaa
     5221 gaaggcggcc aatttgaagg gatgttctat gataaagcta ataaagccgt tactgattta
     5281 ttaacagaaa aaggtgcact attaaaatta gactttatta cacatagcta tccacacgac
     5341 tggagaacaa aaaaacctgt aatcttccgt gctacaccac aatggtttgc ctcaatcagt
     5401 aaagtaagac aagatatttt agatgcaatc gaaaatacaa acttcaaagt aaattggggt
     5461 aaaacacgta tttacaatat ggttcgtgac cgtggcgaat gggttatttc tcgtcaacgt
     5521 gtgtggggtg taccgttacc agtattttat gctgaaaatg gcgaaattat catgacgaaa
     5581 gaaacagtga atcatgttgc tgatttattt gcagaacacg gttcaaatat ttggtttgaa
     5641 agagaagcga aagacttact accagaagga tttacacatc caggcagccc taacggtaca
     5701 tttactaaag aaacagacat tatggacgtt tggtttgatt ctggttcatc acaccgtggc
     5761 gtgttggaaa caagaccgga attaagtttc ccagcggata tgtatttaga aggtagtgac
     5821 caatatcgtg gttggttcaa ctcttctatc acaacttcag ttgctacaag aggagtatca
     5881 ccttataaat tcttactttc tcatggtttt gttatggacg gtgaaggtaa gaaaatgagt
     5941 aaatctttag gtaatgtgat tgtacctgac caagtggtta aacaaaaagg tgctgatatt
     6001 gcgagacttt gggtaagtag tacggactat ttagctgatg ttagaatttc tgatgaaatt
     6061 ttaaaacaaa catctgatgt ttatcgtaaa atcagaaata cattaagatt tatgttaggt
     6121 aacattaacg atttcaatcc tgacacagat agcattcctg aatcagagtt attagaagtg
     6181 gatcgttact tgctaaatcg tttacgtgaa tttactgcaa gtacgattaa caactatgaa
     6241 aactttgact acttaaatat ttatcaagaa gttcaaaact ttatcaatgt tgagttaagt
     6301 aatttctatt tggattacgg taaagatatt ttatatattg aacaacgtga ttctcatatc
     6361 cgtcgtagta tgcaaacagt gttatatcaa attttagttg atatgacgaa gttgttagca
     6421 ccaatcttag tgcatacagc tgaagaagtt tggtctcata caccacatgt taaagaagaa
     6481 agtgttcact tagcagacat gcctaaagtt gtagaagtag atcaagcttt attggataaa
     6541 tggcgtacat ttatgaattt acgtgatgat gtgaaccgtg cattagaaac tgctcgtaat
     6601 gaaaaagtta ttggtaaatc attagaagct aaagttacga ttgctagtaa cgataaattt
     6661 aatgcatctg aattcttaac ttcatttgat gcattacatc aattatttat cgtgtcacaa
     6721 gttaaagttg tagataagtt agacgatcag gcaacagctt atgaacatgg tgatattgtc
     6781 atcgaacatg cagatggtga aaaatgtgaa agatgttgga actattcaga ggatcttggt
     6841 gctgttgatg aattgacgca tctatgtcca cgatgccaac aagttgtaaa atcacttgta
     6901 taattgaaat tgtataaagt actcatacag atgatataaa ttaaagctct cttcataatc
     6961 atgttgtagt ttttgttgac atgatgaaga gagttttttt gtgaataaaa aaatgaccaa
     7021 gttaccggtc atatatgtaa aaaatgtgcg atttactaaa ataaaaatta ttcaggaatg
     7081 gtacaaattc tctgaggcat ataaatgcgt tatagttgct attctcaatt atgttcgcga
     7141 taattttaag taaaagtaag cacagatatt gaatttgata ggagttaatt gaatgtatca
     7201 taacagtaac gcaaactttg tcaatggtat cactttaaat gtgagagata agaatgaatt
     7261 aaagccattt tatgaggaca tattaggatt aaatattata aatgagacat taacatcgat
     7321 acaatatgaa gtaggtcaaa ataatcatgt cattacactt gttgaattac aaaatggacg
     7381 tgaaccttta atgtccgaag cgggactgtt tcatatcgca attaaactac ctcaaattag
     7441 tgatttagct aatttactaa ttcatttaag cgaatatgat attccagtta acggaggtat
     7501 acagcctgct tcgttatcat tattttttga agacccggaa ggaaacggtt ttaaatttta
     7561 tgttgataaa gacgaagcgc aatggacgag gcaaaataat ttagtaaaaa ttgatattag
     7621 accattaaat gtaccgagat tagtgagtca tgcaacaaaa ttgttatggt taggtattcc
     7681 agatgacgct attataggtg cattgcatat taagacaatt catttatcag aggtaaaaga
     7741 gtactacctc gattattttg gattagagca atcggcatat atggatgatt attcaatatt
     7801 tttagcatcg aatggctatt atcaacattt ggccatgaat gattgggtat cagcaacgaa
     7861 acgtgtagaa aattttgata cgtatggatt agcaattgtt gactttcatt atcctgaaac
     7921 aacacattta aatttacaag gtccggatgg tatctattat cgctttaatc atatcgaagt
     7981 tgaagattag tatatacttt gaatggacga accatataat gaatcgtttt taatgatctt
     8041 tttatacaag ttatgaagga ggctgggaca ttaagttctt aggcaatg
//
LOCUS       DALAGD010000117         7755 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.118, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000117 DALAGD010000000
VERSION     DALAGD010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7755
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.118"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            201..1703
                     /locus_tag="O2701_002417"
     CDS             201..1703
                     /locus_tag="O2701_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCZ4294289.1"
                     /translation="MRVAIIGMGTAGVSVLRELVKHPKFNQLDIDLYDDKVNMGQGVP
                     FQNDSSELLINMPSKKMSLNLDDETEFWKWYKQQTDFNFDEPAYLPRFVFGHYMKSYL
                     SMFTKKYPNISTNYNKVQEIYTNSNIDETNLTYYICTTNSGQSWQAYDYVFLTCGTFA
                     YHDPYNLKGKKGYIATPYPTYNTLDEVNELDDIAIIGTGLASLDVVRYVAAHHPKLPI
                     TMTSRSAHLPSVRGTMIDVTFKYLTKDKLNDIKKHHFGNAPLDTLVSLFLKECADYDI
                     DFKKLVHRRTGNHIADLKYDLARPTEMGIFQSMIEHLKENLNWIWNSLSIEDQHQFNQ
                     KYSKMIQLNSNPMPPRTAELIIELIENKSLILKKDLEDVKHDGKLYYFSYKNQESVDI
                     YNVVINATGAKSHLNELDQDDQLIRNLENRQIVQAHPMGGIQIIPETNQVISPRFGTL
                     TNMIAIGQMTNGVNKLRNGVKMIVEQVAHTVSQLYDALESNEQQQRSDNQ"
     regulatory      1922..2057
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            2184..3227
                     /gene="ribD"
                     /locus_tag="O2701_002418"
     CDS             2184..3227
                     /gene="ribD"
                     /locus_tag="O2701_002418"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCZ4294290.1"
                     /translation="MSQFMDYAIQLANMVQGQTGVNPPVGAVVVKEGRIVGIGAHLRK
                     GDKHAEVQALDMAQQNAEGATIYITLEPCSHFGSTPPCVNKIIDCKIAKVVYATKDNS
                     LDTHGDETLRAHGIEVECVDDERASQLYQDFFKAKAKQLPQITVKVSASLDGKQANDN
                     GQSQWITNKEVKQDVYKLRHRHDAVLTGRRTVELDDPQYTTRIQDGKNPIKVILSKSG
                     NIHFNQQIYQDESTPIWIYTENPNLTTNQTHIEIIYLKSCDLTTILHNLYKRGVGTLL
                     VEAGPTTTSEFLQSNYIDEFILYYAPKLIGGSGNYQFYQTNDVIEIPDANQFEIVHSE
                     LLNQNVKLTLRKK"
     gene            3234..3866
                     /locus_tag="O2701_002419"
     CDS             3234..3866
                     /locus_tag="O2701_002419"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCZ4294291.1"
                     /translation="MFTGIVEEIGVVKSVQIRQSVRTIEIEAHKITADMHIGDSISVN
                     GACLTVIDFNQTSFTVQVIKGTENKTYLADVKRQSEVNLERAMSGNGRFGGHFVLGHV
                     DELGTVSKINETANAKIITIQCSQHINNQLVKQGSITVDGVSLTVFDKHDNSFDIHLI
                     PETRRSTILSSKKLGDKVHLETDVLFKYVENILNKDKDQLSVDKLRAFGF"
     gene            3877..5058
                     /gene="ribB"
                     /locus_tag="O2701_002420"
     CDS             3877..5058
                     /gene="ribB"
                     /locus_tag="O2701_002420"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCZ4294292.1"
                     /translation="MQFDNIDSALMALKNGEPIIVVDDENRENEGDLVAVTEWMNDNT
                     INFMAKEARGLICAPVSKDIAQRLDLVQMVDDNSDIFGTQFTVSIDHVDTTTGISAYE
                     RTLTAKKLIDPSSEAKDFNRPGHLFPLVAQDKGVLARNGHTEAAVDLAKLTGAKPAGV
                     ICEIMNDDGTMAKGQDLQKFKEKHQLKMITIDDLIEYRKKLEPEIEFKAKVKMPTDFG
                     TFDMYGFKATYTDEEIVVLTKGAIRQHENVRLHSACLTGDIFHSQRCDCGAQLESSMK
                     YINEHGGMIIYLPQEGRGIGLLNKLRAYELIEQGYDTVTANLALGFDEDLRDYHIAAQ
                     ILKYFNIEHINLLSNNPSKFEGLKQYGIDIAERIEVIVPETVHNHDYMETKKIKMGHL
                     I"
     gene            5071..5535
                     /locus_tag="O2701_002421"
     CDS             5071..5535
                     /locus_tag="O2701_002421"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCZ4294293.1"
                     /translation="MNFEGKLIGKDLKVAIVVSRFNDFITGRLLEGAKDTLIRHDVNE
                     DNIDVAFVPGAFEIPLVAKKLASSGNYDAVITLGCVIRGATSHYDYVCNEVAKGVSKV
                     NDQTNVPVIFGILTTESIEQAVERAGTKAGNKGAEAAVSAIEMANLLKSIKA"
     gene            complement(5657..6658)
                     /locus_tag="O2701_002422"
     CDS             complement(5657..6658)
                     /locus_tag="O2701_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase"
                     /protein_id="HCZ4294294.1"
                     /translation="MALLKNFFIGLSNNSFLNNAAKKVGPRLGANKVVAGNTIPELIN
                     TIEYLNDKNIAVTVDNLGEFVGTVEESNHAKEQILTIMDALHQHGVKAHMSVKLSQLG
                     AEFDLELAYQNLREILLKANTYNNMHINIDTEKYASLQQIVQVLDRLKGEFRNVGTVI
                     QAYLYDSHELVDKYQDLRLRLVKGAYKENESIAFQSKEDVDANYIKIIEQRLLNARNF
                     TSIATHDHRIINHVKQFMKENHIEKDRMEFQMLYGFRSELAEEIANEGYNFTIYVPYG
                     DDWFAYFMRRLAERPQNLSLAVKEFVKPAGLKRVGIIAALGATVMLGLSTIKKLCRK"
     gene            6770..6889
                     /locus_tag="O2701_002423"
     CDS             6770..6889
                     /locus_tag="O2701_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294295.1"
                     /translation="MMLISFIYNNMDNNNTINRNLHSEFAIIMLLKINEKGAV"
     gene            6892..7719
                     /locus_tag="O2701_002424"
     CDS             6892..7719
                     /locus_tag="O2701_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCZ4294296.1"
                     /translation="MWKWEAENDAKGVVVIAHNILEHTGRYAYVITMLRRNGYHVIMG
                     DLPGQGQTSRANKGQIENFQTYHESLLDWLKIANEYKIPTYVLGVGLGGLILLNLLEK
                     VELPIEGMMLISPMLELQKNGKNRKDKLVSNIGKISKDTRFNVGVEPKDLTRNLEIVE
                     ETVNDGLMLKKATYHWYNTINETMKDTMAHIHDIQPMPTLLMYGTKDLIVDTRAIDEF
                     KEKYQTPELYFKAWQGFYHEVHNEPERDEVMRYILTFLNNSVNTMGFIVEDDEIVEI"
BASE COUNT         2790 a         1083 c         1433 g         2449 t
ORIGIN      
        1 ttcaatttaa ttcacctcgt taaaaatatt ttaacacaaa cactaaggtt gatattaaat
       61 gaaaagtttt aaatggttta atggttgtga aagtgaaata atagcttacc ttaataaatt
      121 ttattggaaa taccaaattg ctataattat aggtaatagt ttaaatataa tattttgaat
      181 tacaaaagga tggatgatat atgcgtgtag caataatagg catgggaact gctggtgtaa
      241 gtgtattgag agagttagtt aagcacccga agtttaatca attagatata gatttatacg
      301 atgataaagt aaatatggga cagggcgtac catttcaaaa tgatagttct gagctgctaa
      361 ttaatatgcc ttcgaaaaag atgagtttaa atttagatga tgagactgaa ttttggaagt
      421 ggtataaaca acaaactgat tttaattttg atgaacctgc atatttaccg agatttgtat
      481 tcggacatta tatgaaatct tatttatcta tgttcacgaa aaaatatcca aatatatcaa
      541 ctaattataa taaagttcaa gagatttata caaattctaa tatcgatgaa acgaatttaa
      601 cttattacat atgtactaca aattcaggac aatcatggca agcgtacgat tatgtatttt
      661 taacatgtgg tacatttgcc taccatgatc catataactt aaagggtaaa aaaggatata
      721 tcgctacgcc ttatccaaca tataatacgt tagacgaagt aaatgaactg gacgatattg
      781 cgattattgg tacaggcctt gccagtttag atgttgtccg ttatgttgct gctcatcatc
      841 caaaattacc aattactatg acgagtcgtt ctgctcattt accaagtgta agaggtacaa
      901 tgatagacgt gacattcaag tacttaacta aagacaaatt aaatgacatt aaaaaacatc
      961 atttcggcaa tgcaccgctt gatactttag tttctttatt tttaaaagaa tgtgctgatt
     1021 atgacattga ttttaaaaaa ttagtacata gacgtacagg aaatcatatt gcagatttga
     1081 agtatgattt agcgcgtcca acagaaatgg gcattttcca aagtatgatt gaacatttga
     1141 aagagaattt aaattggata tggaatagct taagtattga agaccaacat caatttaatc
     1201 aaaaatactc aaagatgatt caattaaatt caaatccaat gcctccaaga actgctgaac
     1261 taattattga attaatagaa aataagtctt taattttgaa aaaagattta gaagatgtga
     1321 aacatgatgg caagttgtat tatttttcat acaaaaacca agaatcagta gacatatata
     1381 atgttgtcat taacgcgaca ggtgcaaaaa gtcatttaaa tgaattggat caagatgatc
     1441 aattaattag aaatcttgaa aatagacaaa ttgtacaagc acacccaatg ggaggcatac
     1501 aaatcattcc ggaaaccaat caagttatta gtccaaggtt tggaacatta acaaatatga
     1561 ttgcgatagg tcaaatgaca aacggtgtta ataaactacg aaacggtgtg aaaatgattg
     1621 tagaacaagt tgcgcataca gtatctcaat tatatgatgc tttagaatcg aatgagcaac
     1681 aacagcgcag tgacaatcaa taatttgtaa ctagaagata ataaagagaa cgctctatag
     1741 agacgaattg aaggtttgat tttaatgtct gttagtaaga atcatatcaa tgagatgcct
     1801 atagtactca gattatatta aattaaaacc gtcattaatt gttttttcag aaaacatata
     1861 gtatcatttt aaatgtagtt gacatactac gtactcaaat aatctataac aatttcatat
     1921 ataattcttt cggggcaggg tgaaattccc aaccggcagt aaataaagcc tgcgacctgc
     1981 taatatgttt catattagtg gctgatctag tgagattcta gagccgacag ttaaagtctg
     2041 gatgggagaa agaatgttaa ttatcgacaa agataatgta gcgtatttgt aaaaatgtgt
     2101 acaaataggc ttatttaacg ataaattttt ctccttgcat cttaattcat gatgtgagga
     2161 tttttgttta tagaggtgat catttgagtc aatttatgga ttatgcgatt caacttgcaa
     2221 atatggtaca aggtcaaaca ggtgttaatc cacccgttgg cgctgttgta gttaaagaag
     2281 gtaggattgt tggtattggt gcacacttga gaaaaggtga caagcatgcg gaggttcaag
     2341 cacttgatat ggcacaacaa aatgctgaag gtgcgacgat ttatattacg ttagagccat
     2401 gtagtcattt tggttcaaca ccaccctgtg ttaacaaaat aattgattgt aagatagcaa
     2461 aagtagtata cgcaacaaaa gacaattcgt tagacacaca tggtgatgag acgttacggg
     2521 ctcacggtat tgaggttgaa tgcgttgatg atgaacgggc atcacaatta taccaagact
     2581 tttttaaagc aaaagcaaag caactgccac aaattacagt gaaagtatct gcaagtttag
     2641 atggtaaaca agcgaatgat aatggacaaa gtcaatggat tactaacaaa gaggttaaac
     2701 aagatgtcta taagttaaga catcgacacg acgcagtgtt aactggaaga cgtacagttg
     2761 aattagatga tccacaatat actacacgta ttcaagatgg aaaaaaccct ataaaagtaa
     2821 tattgtctaa gtctgggaat attcatttta atcagcaaat ttatcaagat gaatcaacac
     2881 caatttggat atatactgaa aatccaaatt taacaaccaa tcaaacacat attgaaatta
     2941 tttacttgaa gtcttgcgat ttaacaacaa ttcttcacaa tttatataaa agaggagttg
     3001 gaactttgct agttgaggca ggtccaacca ctacttcaga atttctccaa tctaattata
     3061 tagatgaatt tattctctat tatgccccga aattaattgg cggatctgga aattatcaat
     3121 tttatcaaac aaatgatgtg attgagatac cagatgcgaa ccaatttgaa attgttcatt
     3181 ccgagttatt aaatcaaaat gttaaattaa ctttacgaaa gaagtgatga tgcatgttta
     3241 ctggcatcgt tgaagaaata ggtgtcgtta aaagtgttca aattcgtcaa tcagtcagga
     3301 cgattgaaat tgaagcacat aagattacgg cagatatgca tattggtgat tccatcagtg
     3361 ttaatggtgc atgtttaaca gtgattgatt ttaatcagac atcttttact gttcaagtaa
     3421 ttaaaggcac tgaaaataaa acctatttag cagatgttaa gcgacaatca gaagtaaatt
     3481 tagagcgtgc catgagtggt aacggtaggt ttggtggaca ttttgtcctc ggtcatgtag
     3541 atgaactagg aacagtttca aaaataaatg aaacagccaa tgcaaaaatt attacgattc
     3601 aatgtagcca acatattaat aatcagttag ttaagcaagg gtctattact gtggatggtg
     3661 ttagtctaac ggtatttgat aagcatgata acagttttga cattcatctt attccagaaa
     3721 cgaggcgttc aacgatttta tcatccaaaa aattaggaga taaagtacat ttagaaacag
     3781 acgttttgtt taaatatgtt gaaaatattt taaataaaga taaagaccaa ttatctgtag
     3841 ataaattaag agcatttggt ttttaggagg ggtagcatgc aattcgataa tattgacagt
     3901 gctttaatgg ctttaaaaaa tggagaacca attattgtag tagatgatga gaatcgtgaa
     3961 aatgaaggtg atttagtagc ggttactgaa tggatgaacg ataataccat taattttatg
     4021 gcgaaagaag caaggggatt aatatgcgca ccagtgtcta aagatattgc acaacgtttg
     4081 gatttggtac aaatggttga tgataactcc gacatctttg gtacgcaatt tacagtgagt
     4141 attgatcatg tagatacaac aacaggaatt agtgcttatg aacgtacatt gactgccaaa
     4201 aagctcattg atcctagtag tgaagctaaa gattttaatc gtcctggtca tttatttcca
     4261 ttagtagcac aagataaagg cgtattagct agaaatggac acacagaagc ggctgttgat
     4321 ttagctaaac ttactggtgc caagcccgct ggtgtcattt gtgagattat gaatgatgac
     4381 ggcacgatgg cgaaaggaca agatttacaa aagtttaaag aaaaacatca attaaagatg
     4441 attacgattg atgatttaat tgaatatcgt aaaaaattag aaccagaaat tgaatttaag
     4501 gcaaaagtga aaatgcctac agatttcgga acatttgata tgtatggttt taaagcgaca
     4561 tacacagatg aagagatagt tgtactgaca aaaggtgcaa ttcgacaaca tgaaaatgta
     4621 cgcttacatt ctgcgtgcct tacaggcgat attttccata gtcaacgttg tgattgtggt
     4681 gctcaacttg aatcgtctat gaagtatatc aatgaacatg gtggcatgat tatttatcta
     4741 cctcaagaag gtcgtggcat aggattgtta aacaaattac gcgcatatga attaattgag
     4801 caaggatatg atacagtaac tgcaaattta gcattaggtt ttgatgaaga tttgcgagat
     4861 tatcatattg ctgcacagat tttaaaatat tttaacatcg aacatatcaa tttattaagt
     4921 aataatccaa gtaaatttga gggattaaaa caatatggca ttgatattgc agaaagaatt
     4981 gaagttatcg taccagaaac ggtacataat catgattata tggaaacgaa aaaaataaaa
     5041 atgggtcatt taatatagga ggattttaac atgaattttg aaggtaaatt aattggaaaa
     5101 gatttgaaag ttgcaatcgt agttagtcga tttaatgatt ttatcactgg aagattactt
     5161 gaaggtgcaa aagatacttt gattcgacat gatgttaatg aagacaatat tgatgtagca
     5221 tttgtgccag gtgcgtttga aattccttta gtagctaaaa aattagcctc atcaggaaat
     5281 tatgatgcag taattacatt aggatgcgta attcgcggtg ctacgtctca ttatgattat
     5341 gtttgtaatg aagtcgcgaa aggtgtttct aaagtgaatg atcaaactaa tgtaccagtc
     5401 atatttggta ttttaacgac tgaaagtatt gaacaagctg tggaaagagc aggtacgaaa
     5461 gctggcaata aaggtgctga agcagcagta agtgcaattg aaatggctaa tttattaaaa
     5521 tctataaaag catagagcac tgttgtgagt tcataatcaa agatttataa atgtttcaga
     5581 actgccaata taacatttag gagcctagaa cattgattat tgttccagac tcctaaatta
     5641 ttgtttatgt cttgctctat ttacggcata attttttaat tgtacttaaa cctaacataa
     5701 ctgtagctcc taaagctgca attatgccaa cacgttttaa gccagcaggt ttcacaaatt
     5761 cttttacagc aagagatagg ttttgtgggc gttctgctaa tcttctcata aaatacgcaa
     5821 accaatcatc gccataaggt acataaatag tgaaattata gccttcattt gcgatttctt
     5881 ctgctaactc tgatctaaaa ccatagagca tttggaattc catacgatct ttttcaatgt
     5941 gattttcttt cataaattgt tttacatgat taatgatgcg atggtcatgt gttgcaattg
     6001 aagtgaaatt gcgtgcgttt aacaaacgtt gttcaattat tttgatgtaa tttgcatcta
     6061 cgtcttcctt agattgaaat gcaattgatt cgttttcttt atatgcacct ttaaccaaac
     6121 gtaatcgtaa atcttggtac ttatcaacta attcgtggct atcgtataaa tatgcttgaa
     6181 ttacagtacc aacatttcta aattcgcctt ttaagcgatc taaaacttga acaatttgtt
     6241 gcaggctagc atatttttca gtatcaatat ttatatgcat attgttgtaa gtatttgctt
     6301 taagtaaaat ctctcttaaa ttttggtaag ctaattctaa gtcgaattct gcacctaact
     6361 gactcaattt aacagacata tgtgccttta cgccatgttg atgaagcgcg tccataattg
     6421 ttaaaatttg ttctttagca tgattacttt cttcaactgt accgacaaat tcccctaaat
     6481 tgtctaccgt aacagcgata ttcttgtcat ttaagtattc gattgtatta attaactctg
     6541 gaattgtatt tccggcaacg actttattgg cgcccaaacg tgggcccact ttttttgctg
     6601 cgttgtttaa aaaactatta ttagataatc cgataaaaaa attctttaat agtgccattg
     6661 ctactcctcc aaacccttgt gtttacaata agtttagcat gaaaaaaata ttcgagaaag
     6721 cgttttcatc aaaaacaatc aaaaataatt atgtataatt acactattat tgatgttgat
     6781 ttcatttata tataataata tggacaacaa taatactata aatagaaatc tacatagtga
     6841 atttgctata attatgttat tgaagattaa tgaaaaaggg gcagtataat aatgtggaag
     6901 tgggaagctg aaaatgatgc gaaaggcgtc gttgttattg ctcataatat attagaacat
     6961 acgggaagat atgcatatgt tattacgatg ttaagaagaa atggctatca tgtgattatg
     7021 ggagatttgc ctggtcaagg tcaaacttca agagctaata aaggacaaat tgaaaacttt
     7081 caaacgtatc acgaaagttt gctagactgg ttaaagatag ctaatgaata taaaattcct
     7141 acttatgtat taggggtagg acttggcggt ttgattttat taaatttact tgaaaaagta
     7201 gaattgccaa tcgaggggat gatgttaatt tctcctatgc tagaacttca aaagaatgga
     7261 aaaaatcgta aagataaact tgtttcaaat ataggtaaaa tttcaaaaga cacacgtttt
     7321 aatgttggtg tagaaccaaa agatttaaca cgtaatttag aaattgttga agaaacagtt
     7381 aacgatggtt taatgttaaa aaaagcaacg tatcattggt ataatacaat taatgagacg
     7441 atgaaagata cgatggctca tattcatgac attcaaccaa tgccaacgct attaatgtat
     7501 ggaacgaagg atttaattgt tgatactagg gcaattgatg agtttaaaga aaaatatcaa
     7561 acacctgaat tatattttaa agcttggcaa ggtttttacc acgaggttca caatgagcca
     7621 gaacgtgatg aagtaatgcg ttatattctg actttcttaa ataacagcgt caatacaatg
     7681 ggctttattg ttgaagatga cgaaattgta gaaatttaat attctaaaaa ggttgggaca
     7741 taaatcccta aaaaa
//
LOCUS       DALAGD010000118         7631 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.119, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000118 DALAGD010000000
VERSION     DALAGD010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7631
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.119"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            372..3326
                     /gene="fdhF"
                     /locus_tag="O2701_002425"
     CDS             372..3326
                     /gene="fdhF"
                     /locus_tag="O2701_002425"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCZ4294297.1"
                     /translation="MQEHLVVTLDGKDYLVEPGTNLLEFIKSQDTFVPSICYNESMGP
                     IQTCDTCTVEIDGKIERSCSTVIDRPMTVNTVNNDVKDAQKEALDRILEKHMLYCTVC
                     DYNNGDCEIHNTMDAWGLQHQTYEYKEKPYEKDYGPFYRYDPNQCILCGRCVEACQDI
                     EVNETIRIDWDREHPRVIWDNDVPINESSCVSCGQCATVCPCNAMMEVNMEGNAGYMT
                     DTEPGSLAAMIDLTKKAEPGYGPLFAISDSEAEMRKERIKKTKTVCTYCGVGCSFEVW
                     TKDREILKVQPSHDSPANKIATCVKGKFSWGHINSDQRLTKPLVRKNGEFHEVEWDEA
                     LNVIADNFTAIKEKHGPDALSFISSSKATNEESYLMQKLARQVIGTNNVDNCSRYCQA
                     PATKGLFRTVGHGGDSGSIEDLEKAAMSVLIGTNTAEAHPVIASRMKRAQKLFGQKIH
                     VFDIRKHEMAERADRFYQPKPGTDLAWLSAVTKYIIDHDLHDKAFIDEWVDDFDEYYK
                     SLETFTMAFAEEATGIPESELIKFAEECAKAESVVICWAMGITQQDIGSDSSTAISNL
                     LLVTGNYRRPGTGAYPLRGHNNVQGCSDMGSMPDKITGYQSIEADDIRAKFEKEYGVK
                     LNPKAGKDNHEMVEGIHDGEVHSLYLYGEDTGIVDSNINFVQAAFEKLDFMVVQDEFL
                     TFTATYADVVLPASPSLEKDGTFTNTERRIQRLYQALEPLGDSKPDWKIFQAIANRLG
                     FDWNYKHPSEIMDEVARLTPLYAGVSYDRLEGFNSLQWPVQPDGTDEPILYLEGFNFD
                     NGKAKLFPLSFDNYFKQDEIYDIHVNNGRLLEHFHEGNMTYQTPMIKYKVPRAFVEIS
                     PELAEDRGIHEGAEVKLISETGEAVLQVHVTDRVKGKEIYIPLNNDAMENGDLGAINL
                     LTNSDVDQYTDTPSYKRTSCRLEVITKRGKSPLNPNNFRVNKKRQPQYSVQVQKKWER
                     SDYVFPGNQVDK"
     gene            3326..3799
                     /locus_tag="O2701_002426"
     CDS             3326..3799
                     /locus_tag="O2701_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1641 domain-containing protein"
                     /protein_id="HCZ4294298.1"
                     /translation="MAERISSKIRRLEKSEEQIKLESLNEVTEAIAANKDSILKAIKL
                     IKTLDDAKLLDALNGAIRGRQVIINKFAVELNKDIYTGLLSNMASMVFLLGELNVSDL
                     SDFLNKVNKGLHVANQASPNAKTTIRSLLGVLKDDDMNRSLTYMLNMLKGMSREE"
     gene            4027..4260
                     /locus_tag="O2701_002427"
     CDS             4027..4260
                     /locus_tag="O2701_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294299.1"
                     /translation="MLKTSAVISNTLLVIGLVCLFMMKLVLAITFFAVSLSISLVVFN
                     MMFRDRTTMKVIVNASFLIVILAIVVAYFLLSK"
     gene            4282..5058
                     /locus_tag="O2701_002428"
     CDS             4282..5058
                     /locus_tag="O2701_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HCZ4294300.1"
                     /translation="MKKNFKLRISTLLLIVILVVFAVLLIVNETKLFKNDVNYSFDEA
                     VSMQQGKGIVQTKEEDGKFVEANNNEIAKAMTISHKDNDMKYMDITEKVPMSESEVNQ
                     LLKGKGILENRGKVFLEAQEKYEVNVIYLVSHALVETGNGKSELAKGIKDGKKRYYNF
                     FGIGAFDSSAVRSGKSYAEKEQWTSPDKAIIGGAKFIRNEYFENNQLNLYQMRWNPEN
                     PAQHQYASDIRWADKIAKLMDKSYKQFGIKKDDIRQTYYK"
     gene            5369..6493
                     /locus_tag="O2701_002429"
     CDS             5369..6493
                     /locus_tag="O2701_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HCZ4294301.1"
                     /translation="MKIAIIGAGIGGLTAAALLQEQGHTIKVFEKNESVKEIGAGIGI
                     GDNVLKKLGNHDLAKGIKNAGQILSTMTVLDDKDRLLTTVKLKSNTLNVTLPRQTLID
                     IIKSYVKDDAIFTNHEVTHIDNETDKVTIHFAEQESEAFDLCIGADGIHSKVRQSVNA
                     DSKVLYQGYTCFRGLIDDIDLKHPDCAKEYWGRKGRVGIVPLLNNQAYWFITINSKEN
                     NHKYSSFGKPHLQAYFNHYPNEVREILDKQSETGILLHNIYDLKPLKSFVYGRTILLG
                     DAAHATTPNMGQGAGQAMEDAIVLVNCFNAYDFEKALQRYDKIRVKHTAKVIKRSRKI
                     GKIAQYRSRLFVAVRNRIMKMMPNALAAGQTKFLYKSKEK"
     gene            complement(6585..7538)
                     /locus_tag="O2701_002430"
     CDS             complement(6585..7538)
                     /locus_tag="O2701_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HCZ4294302.1"
                     /translation="MEKVYVAGAIPEVGLKLLQEHFEVEMYEGKGLVDKDTLIKGVKN
                     ATALISLLSTNVDKDVIDAGKDLKIIANYGAGFNNIDIEYAREKSIDVTNTPKASTNA
                     TADLTIGLVLAVARRIVEGDQLSRTTGFDGWASLFFRGREVSGKTIGIIGLGEIGSAV
                     ARRARAFDMDVLYTGPNRKEEKEREIGAKYVDLDTLLKNADFITINAAYNPKMHHLID
                     TEQFKMMKSTAYLINASRGPIVHEQALVQALKDNEIEGAALDVYEFEPDITDDLKSLN
                     NVVLTPHIGNATFEARDMMSKIVANAAISAVQGEKPQFVVN"
BASE COUNT         2715 a         1077 c         1466 g         2373 t
ORIGIN      
        1 tgggttatta attttcagaa aaagggtatt tataaatagt ataacgattt ttaggtgagg
       61 tgattattaa atattgtcat taataaggtt gtgaaagata tggcaaaaag cgtgaaacag
      121 ataattggaa aactaataat gataattatt ttcaattaaa caaaaagatg atttaatttc
      181 gcgcaaaata tttcaaataa aaatgattgt aaaaaggcaa aatacaaaat tttacttaac
      241 aactagtaca taaagtaata caattaaatt aattctatct gaaagatgtg tggggcatcg
      301 ttattttagg tggatatgag caatttatta aaagtcattt acggaaaata tatatagacg
      361 gggtgagtaa tatgcaagaa catttggtgg ttacacttga tggaaaagat tatcttgtag
      421 aaccaggtac gaatttactt gaatttatta aatcacaaga tacttttgta ccttcaattt
      481 gttataacga gtcgatggga ccaattcaaa catgtgatac atgtactgtt gagattgacg
      541 gtaaaattga acgttcatgt agtacggtga ttgatcgtcc aatgactgta aatactgtga
      601 acaatgatgt gaaagatgct caaaaagaag cgcttgatcg aattttagaa aagcatatgc
      661 tgtattgtac agtatgtgat tataataatg gtgattgtga aattcataat acgatggatg
      721 catgggggct tcagcatcaa acgtatgaat ataaagagaa gccatacgaa aaagattacg
      781 gcccatttta tcgttatgat ccaaatcaat gtatcttatg tgggcgttgt gtagaagcat
      841 gtcaagatat cgaagtgaat gaaactatta gaattgattg ggatcgtgaa catccacgtg
      901 ttatttggga taatgatgta ccgattaatg agtcttcatg tgtatcttgt ggtcaatgtg
      961 cgacggtatg tccatgtaat gccatgatgg aagtgaatat ggaaggtaat gcgggttata
     1021 tgactgatac ggaacctggt tcattagcag caatgattga tttaactaaa aaagcagaac
     1081 ctggttatgg tccactattt gcaatctcag attcagaagc agaaatgcgt aaagagcgta
     1141 ttaagaaaac taaaacagtg tgtacttatt gtggtgtggg ttgttcattt gaagtttgga
     1201 ctaaagatag agaaatttta aaagtacaac catcacatga ttctccagca aataaaattg
     1261 cgacttgtgt taaaggtaag ttttcatggg gacatattaa ttcagatcaa cgattaacta
     1321 aaccactagt aaggaaaaat ggtgagttcc atgaagtaga atgggatgaa gctttaaacg
     1381 tcattgcaga taattttaca gctattaaag aaaagcatgg cccagatgca ctatcattca
     1441 tttcttcttc taaagcgacg aatgaagaat cgtatttaat gcaaaaatta gcaagacaag
     1501 ttattggcac aaataacgtt gataactgtt caagatattg ccaagcacct gcaacaaaag
     1561 gcttatttag aacggttgga cacggcggtg actcaggtag tattgaagat ttagaaaaag
     1621 cggcaatgtc tgtattgata ggtactaata cagccgaagc tcatccagtt atcgcatcac
     1681 gcatgaaacg tgcacaaaaa ttatttggtc aaaaaataca tgtatttgat attagaaaac
     1741 atgaaatggc agaacgtgcg gatcgttttt atcaacctaa accaggtacg gatttagcgt
     1801 ggttaagtgc agtgactaag tatattattg atcatgattt acacgataaa gcatttattg
     1861 atgagtgggt agatgatttt gatgaatatt acaaatcatt agaaacattt acaatggctt
     1921 ttgctgaaga agcaacaggt attcctgaat cagaattgat taaatttgct gaagaatgtg
     1981 ctaaagctga atctgttgta atttgttggg caatgggtat tacacaacaa gacattggta
     2041 gtgactcaag tacagcgatt tcaaacttat tattagtaac aggtaattat cgtcgtcctg
     2101 gtactggtgc gtatccatta cgtggacata ataatgttca aggatgtagt gatatgggaa
     2161 gtatgcctga taagattact ggttatcaaa gtattgaagc ggatgatatt cgcgctaaat
     2221 ttgaaaaaga atatggcgtt aaattgaatc caaaagctgg taaagataat catgaaatgg
     2281 tagaaggtat acatgacgga gaagtacact cattgtactt atatggtgaa gatacgggta
     2341 ttgtggattc aaatattaat tttgtacaag ctgcgtttga aaaattagat ttcatggtag
     2401 tccaagatga atttttaaca ttcacagcaa catacgcaga tgttgtattg ccagcaagtc
     2461 cttcacttga aaaagacggt acttttacaa ataccgaacg tcgtattcaa cgtttatacc
     2521 aagcattaga acctcttggt gattcaaaac ctgactggaa aattttccaa gcaattgcta
     2581 atagattagg atttgattgg aattacaagc atcctagtga aattatggat gaagtagcac
     2641 gcttaacacc tctatatgct ggggtaagtt atgatcgttt agaaggattc aatagtttac
     2701 aatggccagt acaacctgat ggtactgatg agcctattct atacttagaa ggattcaatt
     2761 ttgataatgg taaagcaaaa ttattcccat tatcatttga taattacttt aagcaagatg
     2821 aaatttatga tattcatgta aacaatggtc gattgttaga gcacttccat gaaggtaata
     2881 tgacttatca aacaccaatg attaaataca aagtgccacg tgcatttgtt gaaatttctc
     2941 cagagcttgc tgaagataga ggcattcatg aaggtgcaga agttaagctg atttctgaaa
     3001 caggagaagc ggtattacaa gttcacgtga cagatcgtgt taaaggtaaa gaaatctata
     3061 ttccattaaa taatgatgcg atggaaaatg gagatctagg tgcaattaac ttattaacta
     3121 atagtgatgt tgatcaatac acggatacac catcttataa acgtactagc tgtcgtttgg
     3181 aggtaattac gaaacgtggt aagtcaccgt tgaaccctaa taatttccgt gtcaataaaa
     3241 aacgtcaacc gcagtacagt gttcaagtac agaaaaaatg ggaacgttca gattatgttt
     3301 tcccgggaaa tcaggtggat aaataatggc tgagagaata tcttctaaaa ttagacgctt
     3361 agaaaaatca gaagaacaaa ttaagttaga aagtttaaat gaagttacag aggcaattgc
     3421 agcgaataaa gatagtatat taaaagcaat caagcttatt aaaacattag atgatgcaaa
     3481 actattagat gctttaaatg gtgcaattcg aggacgtcaa gtcatcataa ataaatttgc
     3541 agttgaactt aataaagata tttatacagg gctattatct aatatggctt caatggtatt
     3601 tttattaggt gaactcaatg tatcagattt aagcgacttc ctaaataaag ttaataaagg
     3661 attacacgtt gccaatcaag cgagtccaaa tgctaagaca actataagaa gtctattggg
     3721 cgtattgaaa gatgacgaca tgaaccgtag tttaacatac atgcttaata tgctaaaagg
     3781 tatgtctcga gaagaataac attacaatga aaaagtgttt ctacatgcaa gcagtgtagg
     3841 agcacttttt ttaatttttg taaaaattaa aaagggtgtt tttgatggaa taaatgaata
     3901 atattgaaaa acaaaaaaat cacgtgctat tgtgaatcaa tcatcgacag gcctatgaaa
     3961 catcatgtat tattatgatt atgtaagtta tttaaataaa aagggtgagt gcatattaat
     4021 tcgagaatgt taaaaacgtc agctgtaatt tctaatacat tattagtcat aggtttagtc
     4081 tgtctattta tgatgaaatt agtgttagct attacttttt ttgctgtttc attatcaatt
     4141 agtctagttg tctttaatat gatgttcaga gatagaacaa cgatgaaagt aatcgttaac
     4201 gcttcttttt taatcgtaat attagcgatt gtagtggcat attttttatt gtctaagtaa
     4261 gaaaaatgag gttttgtcta tatgaagaag aatttcaagt tacgcatttc aacgctacta
     4321 ttgatagtta ttttagttgt ttttgctgta ttactcatcg tgaatgaaac taaattgttt
     4381 aaaaatgatg tgaattactc ttttgatgag gctgtttcaa tgcaacaagg gaaaggtatt
     4441 gtacagacaa aagaagagga tggtaaattt gttgaagcaa ataataatga aattgctaaa
     4501 gcaatgacta tttcacataa agacaatgat atgaagtata tggatatcac agaaaaagtg
     4561 ccaatgtcgg aatctgaagt taaccaattg ctaaaaggta aggggatttt agaaaatcga
     4621 gggaaagttt ttctagaagc tcaagaaaaa tatgaggtta atgtcattta tcttgttagc
     4681 catgcattag tagaaacagg taacggcaaa tcagaattag caaaaggcat taaagatggg
     4741 aaaaaacgct attacaactt ttttggtata ggagcattcg atagtagtgc tgttcgtagt
     4801 gggaaaagtt atgctgaaaa ggaacaatgg acatcaccag ataaggcgat tattggtggt
     4861 gcaaagttca ttcgtaatga atattttgaa aacaatcaac tgaatttata tcaaatgcga
     4921 tggaatccag aaaatcctgc gcaacatcaa tatgcgagtg acattcgctg ggcagataaa
     4981 attgccaaat taatggataa atcctataag cagtttggta taaagaaaga tgatattaga
     5041 caaacatatt ataaataaga catcggtgct taaaggagct ggaacaattt attgtttcga
     5101 gctcctttag cgcattctga gtgtgttagt taaatggatt ttaacctaac aaaaaacgct
     5161 atatagcatc aaatatgcta tatcccacat cattgttaca aatgtacatg atgtaaatga
     5221 atattgctgt ctaaatgtgc atgtaatata caatggtgca gataatacac ttaagtcctt
     5281 aaaaatgaaa cgttagttcc gagagtcatt tttaaacaat agtgcatgtg ataaaataga
     5341 aaagaatgaa aaatatagag gtgacaatat gaagatagca attataggtg caggcatcgg
     5401 tggattaaca gctgctgcat tattacaaga acaaggtcat actattaaag tctttgaaaa
     5461 aaatgagtca gttaaagaaa ttggcgctgg gattggtatc ggagataatg tgcttaaaaa
     5521 actaggtaat catgacttag ctaaaggtat taaaaatgct gggcaaatct tatctacaat
     5581 gacagtgtta gatgacaaag atcgcctgtt aactactgtt aaattaaaaa gtaatacatt
     5641 gaatgtgacg ttaccacgcc aaacattaat tgacattatt aaatcttatg taaaagatga
     5701 cgcaatattt acaaatcatg aagtcacgca tatagataat gagacagata aagttaccat
     5761 acatttcgcg gaacaagaaa gtgaagcatt tgatttatgt attggtgctg atggaattca
     5821 ttctaaagtg agacaatctg taaatgctga cagtaaagta ttatatcaag ggtatacatg
     5881 ctttagaggt ttaattgatg atattgattt aaagcatcca gattgtgcaa aagaatactg
     5941 gggaagaaaa ggaagagtag gtattgttcc gttattaaat aatcaagcat attggttcat
     6001 tacaattaac tcgaaggaaa acaatcataa atatagttcg tttggtaaac ctcatttgca
     6061 agcatacttt aatcactatc caaatgaagt tagagagatc ttagacaaac aaagtgaaac
     6121 aggtatctta ttgcataata tttatgattt gaaaccactc aaatcttttg tttatggtcg
     6181 tactatttta ctaggagatg cagcacatgc gacaacgcct aatatggggc aaggtgctgg
     6241 acaagcaatg gaagatgcta tcgtattagt aaattgtttt aacgcatacg actttgaaaa
     6301 agcattacag cgttatgata aaatacgtgt caaacatact gcaaaagtaa ttaagcgttc
     6361 tagaaaaatc ggtaaaattg cccaatatcg tagtcgttta tttgttgcag ttagaaatcg
     6421 tattatgaaa atgatgccaa atgcattagc agctggacaa actaaattct tatataaatc
     6481 gaaagaaaaa taatacaaca atatgaaaac ccccgtatgt tgaaacgaga gctcaacata
     6541 tgggggttct tgtttttata atgttattat aataaattca attattagtt aacgacaaat
     6601 tgtggtttct caccttgaac ggcactaatt gcagcattag caacaatttt agacatcatg
     6661 tcacgtgctt caaatgtagc attaccaata tgcggtgtta atactacatt attaagtgat
     6721 tttaagtcat cggtaatatc tggttcaaat tcatatacat caagtgcagc accttcaatt
     6781 tcattatctt tcaatgcttg cactagtgct tgttcgtgca cgattggacc acgagaggca
     6841 ttgattaaat acgccgtaga tttcatcatt ttaaattgtt ctgtatcaat taaatgatgc
     6901 attttaggat tataagcagc gttgatagtg ataaaatctg cattctttaa tagtgtatct
     6961 aaatctacat attttgcacc gatttctcgt tctttttctt ctttgcgatt aggtccagtg
     7021 tatagcacat ccatgtcaaa tgctcttgca cgacgagcta ctgcactacc aatttcacct
     7081 aaaccgataa tgccgattgt tttcccagat acttctctac ctctgaaaaa taaagatgcc
     7141 catccatcaa atccagttgt acgtgataat tggtcccctt caacaatacg acgcgctact
     7201 gcaagtacta atccaattgt taaatcagca gtcgcgtttg ttgatgcttt aggtgtgttt
     7261 gtaacatcta tacttttttc tcgggcatac tcgatatcaa tattattaaa accagcgcca
     7321 tagttggcaa tgatttttaa gtctttacca gcatcgataa catctttatc aacgtttgta
     7381 gataataaac taattaaggc agtcgcgttt ttaacacctt taattaaagt gtctttatcg
     7441 actaatcctt taccttcata catttcaact tcaaaatgtt cttgtaaaag ttttaaacct
     7501 acttctggta ttgcaccagc aacataaact ttttccataa aagatcactc cttttatctt
     7561 agtatagtag aagattagac agtatacaac tatgtcatga tgtcttgtgt atcaatgatg
     7621 taagcgcgta c
//
LOCUS       DALAGD010000119         7231 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.120, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000119 DALAGD010000000
VERSION     DALAGD010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7231
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.120"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..1173
                     /locus_tag="O2701_002431"
     CDS             <1..1173
                     /locus_tag="O2701_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCZ4294303.1"
                     /translation="FPGFEGEWEEKQLGDLTDRVIRKNKNLESKKPLTISGQLGLIDQ
                     TEYFSKSVSSKNLENYTLIKNGEFAYNKSYSNGYPLGAIKRLTRYDSGVLSSLYICFS
                     IKSEMSKDFMEAYFDSTHWYREVSGIAVEGARNHGLLNVSVNDFFTILIKYPSLEEQQ
                     KIGKFFSKLDRQIELEEQKLELLQQQKKGYMQKIFSQELRFKDENGEDYPDWENSKIE
                     KYLKERNERSDKGQMLSVTINSGIIKFSELDRKDNSSKDKSNYKVVRKNDIAYNSMRM
                     WQGASGKSNYNGIVSPAYTVLYPTQNTSSLFIGYKFKTHRMIHKFKINSQGLTSDTWN
                     LKYKQLKNINIDIPVLEEQEKIGDFFKKMDILISKQKMKIEILEKEKQSFLQKMFL"
     gene            1556..2228
                     /locus_tag="O2701_002432"
                     /pseudo
     CDS             1556..2228
                     /locus_tag="O2701_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498986.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL11"
     gene            2250..3758
                     /locus_tag="O2701_002433"
     CDS             2250..3758
                     /locus_tag="O2701_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKLRK protein"
                     /protein_id="HCZ4294304.1"
                     /translation="MRENFKLRKMKVGLVSVAITMLYIMTNGQAEASENQNALISNIN
                     VDNQEKQNNVNQAVQPQNNTNETSKVPANFVKLNDIKPGDTSIQGTTLPNQFILLTID
                     KKDVSSVEDSDSSFVMSDKDGNFKYDLNGRKIVHNQEIEVSSSDPYLGDDEEDEEVEE
                     TSTEEVGAEEESTEAKATYTTPRYEKAYEIPKEQLKEKDGHHQVFIEPITEGSGIIKG
                     HTSVKGKVALSINNKFINFETNANGGPNKEEAKSGSEGIWMPIDDKGYFNFDFKTKRF
                     DDLELKKNDEISLTFAPDDEDEALKSLIFKTKVTSLEDIDKAETKYDHTKVEKVKVLK
                     DVKEDLHVDEIYGSLYHTEKGKGILDKEGTKVIKGKTKFANAVVKVDSELGEGQEFPD
                     LQVDEKGEFSFDVDHAGFRLQNGETLNFTVVDPITGELLSGNFVSKNIDIYESPEEKA
                     DREFDERMENTPAYHKLHGDKIVGYDTNGFPITWFYPLGEKKVERKAPKLEK"
     gene            complement(3861..4169)
                     /gene="spn"
                     /locus_tag="O2701_002434"
     CDS             complement(3861..4169)
                     /gene="spn"
                     /locus_tag="O2701_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myeloperoxidase inhibitor SPIN"
                     /protein_id="HCZ4294305.1"
                     /translation="MKFKKVLVATAMVGVLATGVVGYGNQADAKVYSQNGLVLHDDAN
                     FLEHELSYIDVLLDKNADQATKDNLRSYFADKGLHSIKDIINKAKQDGFDVSKYEHVK
                     "
     gene            4537..5310
                     /gene="lpl1"
                     /locus_tag="O2701_002435"
     CDS             4537..5310
                     /gene="lpl1"
                     /locus_tag="O2701_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl1"
                     /protein_id="HCZ4294306.1"
                     /translation="MGYLKRFALYISVMILIFAIAGCGKGNETKEDSKEEQIKKSFAI
                     TLDMYPIKNLEDLYDKEGYRDSEFKKGDKGMWTIYTDFAKSNKPGELDDEGMVLNLDR
                     NTRTAKGHYFVTTFYRNGKLPDEKNYKIEMKNNKIILLDEVKDDKLKQKIENFKFFGQ
                     YANLKELRKYNNGDVSINENVPSYDVEYKMSNKDEIVKELRSRYNISTEKSPILKMHI
                     DGDLKGSSVGYRKLEIDFSKRENSKLSVIEFLSYKPAKK"
     gene            5341..6141
                     /gene="lpl2"
                     /locus_tag="O2701_002436"
     CDS             5341..6141
                     /gene="lpl2"
                     /locus_tag="O2701_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl2"
                     /protein_id="HCZ4294307.1"
                     /translation="MRYLKKLAWFISVIILGIFIIGCDSSSDTGEKAKEDSKEEQIKK
                     SFAKTLDMYPIKNLEDLYDKEGYRDSEFKKGDKGMWMIYTDFAKSNKPGVLDNEGMIL
                     NLDRNTRTAKGYYFVDTIYDNHENSYSKNYRVEMKNNKIILLDKVEDQKLKERIENFK
                     FFGQYADFKSLKSYNNGDVSINSNVPSYDAKFKMSNKDENVKQLRSRYNIPTEKAPIL
                     KMHIDGDLKGSSVGYKKLEIDFSKEENSELSVVDSLNFQPAKKNKDDE"
     gene            6160..6967
                     /locus_tag="O2701_002437"
                     /pseudo
     CDS             6160..6967
                     /locus_tag="O2701_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
     gene            7013..>7231
                     /gene="lpl4"
                     /locus_tag="O2701_002438"
     CDS             7013..>7231
                     /gene="lpl4"
                     /locus_tag="O2701_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl4"
                     /protein_id="HCZ4294308.1"
                     /translation="MMGYLKRLVLYIVIMVMSVFIIGCDKSSDTAEKSKEDSKETQIK
                     KSFAKTLDMYSIKNLEELYDKEGYRDGEF"
BASE COUNT         2981 a          805 c         1323 g         2122 t
ORIGIN      
        1 ttcccagggt ttgaaggcga atgggaagag aagcagttag gggatcttac agatagagta
       61 attaggaaaa ataaaaactt agaatcgaaa aagcctttaa caatatccgg acagttaggt
      121 ttaattgatc aaacagaata ttttagtaaa tcagtttcgt cgaaaaatct agaaaattat
      181 acactaataa agaatggaga attcgcgtat aacaaaagtt attctaatgg atacccatta
      241 ggggctatta aaagattaac tagatatgat agtggtgtat tgtcctcttt gtatatttgt
      301 ttttctatta aaagtgaaat gtctaaagac ttcatggaag catattttga ttcgacacac
      361 tggtatagag aagtttctgg aattgcagtt gagggtgcaa gaaatcacgg attattaaat
      421 gtttctgtga atgatttttt tactattcta attaaatatc caagtttaga agaacagcaa
      481 aaaataggca agttcttcag caaactcgac cgacaaattg aattagaaga acaaaagctt
      541 gaattacttc aacaacagaa aaaaggctat atgcagaaaa ttttctcaca ggaactgcga
      601 ttcaaagatg agaatggtga agattatcca gattgggaaa atagcaaaat agaaaaatat
      661 ttaaaagaga gaaacgaacg ttctgacaaa gggcaaatgc tttcagtaac tataaatagt
      721 ggcattataa aatttagtga attggataga aaagataatt caagtaaaga taaaagtaat
      781 tataaagtag ttaggaaaaa tgatattgca tataattcta tgagaatgtg gcaaggggct
      841 agtggtaaat caaattataa tgggattgtt agccctgcat atactgtgct ttatccaaca
      901 caaaatacta gctcattatt tattggatat aagtttaaaa cacatagaat gattcataaa
      961 tttaaaatta attcacaagg attaacatca gatacatgga acttaaaata taaacaatta
     1021 aaaaatataa atatagatat acctgtattg gaggaacaag aaaagatagg tgatttcttt
     1081 aaaaaaatgg atatattgat aagtaaacag aaaatgaaaa ttgaaatatt agaaaaagag
     1141 aaacaatcct ttttacaaaa aatgttctta taactttgat aaatacatag attgcataag
     1201 aataaaattt gtataattta acataaaagt tgtaaaagta aagtgaatta aaaacgaaca
     1261 ttaaatttag gcactgtgaa agcgcagtgt cttttttgtg tcgaaattgt gtacagaata
     1321 agtagttaaa taaagattaa gttgagataa agtgttattc gtaaataaaa gagagtagat
     1381 cgataggaat tgaatgatat tagttaacta tttattaaat tacttaataa tgattaattt
     1441 ttagttaaag taagtttaat gtgaagcacg accattgctc attataatga atgaggattg
     1501 ttcgtattgc gtaatagaat aaatcaaata gactaaaaat tgggagcata gaattatgaa
     1561 attaaaaaat attgctaaag caagtttagc actagggatt ttaacaacag ggatgattac
     1621 aactactgct cagccagtaa aagcaagtac attagaggtt agatcacaag ctactcaaga
     1681 cttgagtgaa tattataata gaccgttctt tgagtataca aatcagtcag gatataaaga
     1741 ggaaggaaaa gtgacgttta ctcctaatta tcaacttata gatgtaactt taactgggaa
     1801 tgaaaagcaa aattttggtg aagatatttc taatgtagat atatttgttg taagagaaaa
     1861 ttctgataga tctggtaact tcaattggtg gtattactaa aacaaacggt tcaaattata
     1921 ttgataaagt aaaagatgta aatttaataa ttactaaaaa catcgatagt gttacatcaa
     1981 cgtcaacatc atctacatat acaattaata aagaagaaat ttcattaaaa gaacttgatt
     2041 ttaaattaag aaagcattta attgataaac ataaccttta taagacagaa cctaaagaca
     2101 gtaaaattcg aattactatg aaagatggtg ggttctacac atttgaattg aataaaaagt
     2161 tacaaacaca ccgtatgggt gatgttattg atggcagaaa tatagaaaaa attgaagtga
     2221 atttataaaa ttattcgagg gagcatatca tgagggaaaa ttttaagtta cgtaaaatga
     2281 aagtcggttt agtatctgtt gcaattacaa tgttatatat tatgacaaac ggacaagcag
     2341 aagcatctga aaatcaaaac gctttaatct ctaatataaa tgtagacaat caggaaaaac
     2401 agaataatgt aaatcaagct gttcagcctc aaaataatac taatgaaaca tcaaaagtac
     2461 cggctaattt tgtcaaattg aatgatatta aaccaggtga tacttctata caaggaacaa
     2521 ctttaccaaa tcaatttata ctattaacta ttgataaaaa agatgtgagc tcagttgaag
     2581 attctgacag cagctttgtt atgtctgata aagatgggaa ttttaagtat gacttaaatg
     2641 gtcgcaaaat tgttcataat caagaaattg aagtgtcttc atcagatccc tatttaggtg
     2701 acgatgaaga agatgaagaa gtagaagaaa cttcaactga agaagttggt gctgaggaag
     2761 aaagtacaga agctaaagct acatatacaa caccgcgata tgaaaaagcg tatgaaatac
     2821 cgaaagaaca gctaaaagaa aaagatggac atcaccaagt ttttatcgaa cctattactg
     2881 aaggttcagg tattattaaa ggccatacct ctgtaaaagg taaagttgct ctatctatta
     2941 ataataaatt tattaacttt gagacaaatg ctaatggtgg tccaaataaa gaagaagcga
     3001 aatctggatc agaaggaatc tggatgccta ttgatgacaa aggatacttt aattttgact
     3061 tcaaaacgaa acgtttcgat gatttagagt taaagaaaaa tgatgagatc tcattaacat
     3121 ttgcacctga tgacgaagat gaggcattga agtcattaat tttcaaaact aaggtaacga
     3181 gtttagaaga tattgataaa gcagaaacta aatatgacca tactaaagtg gaaaaagtaa
     3241 aagtattgaa agatgttaaa gaagatttac atgtagatga aatttacgga agcttatatc
     3301 atacagaaaa aggtaaaggt attcttgata aagaaggtac taaagtaatt aaaggtaaga
     3361 ctaaattcgc aaatgcagtt gtgaaggtag actctgaact aggtgaaggt caagaattcc
     3421 ctgatttgca agtcgatgaa aaaggtgaat tcagctttga tgtagatcat gctggattta
     3481 gattacaaaa tggagaaaca ctaaacttca cagtagttga tcctattaca ggtgaattat
     3541 taagtggaaa ttttgtttct aaaaacatag atatttatga atctcctgaa gaaaaagcag
     3601 atcgtgagtt tgatgaaaga atggaaaaca cacctgcata tcataaatta catggtgata
     3661 aaattgtcgg ctacgatact aacggattcc cgattacctg gttttatcca ttaggtgaaa
     3721 agaaagttga acgtaaggca ccaaaattag aaaaataatt aaataaaaca gcttaatgat
     3781 gtaatgaaat tagtgagtta atcactgact tctacgtcat tgagctgttt tttgtgcttt
     3841 gttacaaagc attattgaat ttattttacg tgttcatatt ttgaaacatc aaagccgtct
     3901 tgtttagctt tgttgataat gtctttgatt gaatgtagtc ctttatcggc gaagtatgat
     3961 cttaagttgt cttttgtagc ttggtcagca ttcttatcta ataacacatc aatataactt
     4021 aattcatgtt ctaagaagtt tgcatcatca tgtagtacga gtccattttg agaataaact
     4081 ttcgcatctg cttgattacc atatccaaca acgccagttg ctaaaacacc taccattgcc
     4141 gtagctacta aaaccttttt aaatttcata tctatcactc ctctaaaaat tgtaactcca
     4201 tcataacact gaatattaag aaaattacgt ttattaagtc gatttaataa tttttaataa
     4261 atagttaaag tgacaaatat tgtttaaatg caattaatct ttaatacgat gcttgaggat
     4321 ttttcctaat aaaaccttga tttcaaaaag ggtttaaatc aaatgaaaca ataataaaaa
     4381 aatgacgcaa tataataata agtacaaatt taattaagaa attaaattga ttgtatatgt
     4441 atattttgga aacgtaaaag agaaatatac aaaataatta attatttata tgaaaagaga
     4501 atataaatga agtataaaac agagagacgt gaagcgatgg gatatttaaa aaggtttgca
     4561 ttgtacataa gcgttatgat tttaatattt gcgatagcag gttgtggcaa aggtaatgaa
     4621 acaaaagaag attcaaagga agaacaaatc aaaaagagct ttgcgataac attagatatg
     4681 tatccaatta agaatctcga ggacttatat gacaaagaag gatatcgaga tagcgaattt
     4741 aaaaaaggtg acaaagggat gtggacgata tatacagatt tcgccaaaag taataaaccg
     4801 ggtgaattgg atgatgaagg tatggtttta aatctggata ggaatactag aaccgctaaa
     4861 ggccattatt ttgttactac attttaccgg aatggtaaac taccagatga aaagaattat
     4921 aaaattgaaa tgaaaaataa taagattatt ttattagatg aagtaaaaga tgataagctc
     4981 aagcagaaaa tagaaaactt taaatttttc ggtcaatatg caaatcttaa agaattgaga
     5041 aaatataata atggtgatgt ttcaattaat gaaaatgttc ctagttatga tgttgaatac
     5101 aaaatgagca ataaagatga aatagttaag gaattaagaa gtcgttataa tatttcgact
     5161 gaaaaatcac ctatattaaa aatgcatatt gatggggacc tgaaaggcag ttctgtaggt
     5221 tatagaaagt tagaaattga cttttcaaaa cgtgaaaaca gcaaattatc agtcattgaa
     5281 tttttaagtt ataaaccagc gaaaaaatag tattgataag gaatattagg gtgtgaaaaa
     5341 atgcgatatc taaaaaagct tgcatggttc ataagtgtta ttattttggg catttttata
     5401 ataggttgtg atagttcaag cgatactggg gaaaaagcaa aagaagattc aaaggaagaa
     5461 caaatcaaaa agagctttgc gaaaacatta gatatgtatc caattaagaa tctcgaggac
     5521 ttatatgaca aagaaggata tcgagatagc gaatttaaaa aaggtgacaa agggatgtgg
     5581 atgatatata cagatttcgc caaaagtaat aaaccgggtg tattagataa tgaaggtatg
     5641 attttaaatt tggatagaaa tacacgtacg gccaagggat attattttgt agatactata
     5701 tatgacaatc atgaaaactc ttatagtaaa aattatagag ttgagatgaa aaacaataaa
     5761 attattttat tagacaaggt ggaagatcaa aaacttaaag aaagaataga aaactttaaa
     5821 tttttcggac aatatgccga tttcaagagt ttgaaaagtt acaacaatgg cgacgtttca
     5881 attaatagta atgttccaag ttatgacgcg aaatttaaaa tgagtaataa agatgaaaat
     5941 gttaagcaat taagaagtcg ttataacatt cctactgaaa aagctccaat attaaaaatg
     6001 catattgatg gggacttaaa aggcagttcc gttggatata aaaagttaga aatagacttt
     6061 tcaaaagaag aaaatagcga attatcagta gtcgattcat taaattttca gcctgccaaa
     6121 aaaaataaag atgatgaatg atagggagga tgtgaaacaa tgtggtcaat aaaaagaatt
     6181 ggaatgtaca caaatgttat aattttgagc gtttttataa taggttgtga tagttcaagc
     6241 aataatgcag aaaatcaaag agaagactca aaagaagaac aaatcaaaaa gagctttgcg
     6301 aaaacgttag atatgtaccc tattaagaat ctcgaggatt tatatgacaa agaaggatat
     6361 cgtgatggtg agtttgaaaa aggcgataag gggacgtggg tattgtattc agctatggta
     6421 tcacaaccaa aaggcgagag tttaaaatca cgaggaatga tattaaagtt agatagaaat
     6481 aagagaactg ctaaaggaag ttatattatt agagaattga aagaagataa aaatcatgat
     6541 gttcaaaaaa atgaaaagaa atatccagtg aaattggtga ataataggat agttttgata
     6601 aaagatgtta aagacaaaaa gttaaaaaaa tgaaatagag tcgtttgaat tattttcaca
     6661 atatggaaac tttaatcatt ttgatcggaa tgagattact aatatttcat ataatcctaa
     6721 tgctcccaat tactctgcag aatataaaat gaagaaaaat gacagaaaca ttcaacagtt
     6781 gaaaaagaga tttaatctaa aaactagcaa gacaccaaaa ttattgttta agggatctgg
     6841 agatataaag gggtcttctg taggatataa ggaaatagaa atcatattta gtagaagtaa
     6901 agaagaaagc atttattatg ttgacagcgt tgagttcgtt ccaagtaaca aaataacaaa
     6961 aagttgatat tagtcaacaa aagcaaatat tgagaataaa gagagttgtg aaatgatggg
     7021 ttatttaaaa agacttgtat tgtatatagt tattatggtt atgagtgttt ttataatagg
     7081 ttgtgataaa tcaagcgata ctgcagaaaa atcaaaagaa gactcgaaag aaacacaaat
     7141 caaaaagagt tttgcgaaaa cgttagatat gtattcaatt aagaatctcg aggaactata
     7201 tgacaaagaa ggctatcgag atggcgaatt t
//
LOCUS       DALAGD010000120         7105 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.121, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000120 DALAGD010000000
VERSION     DALAGD010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7105
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.121"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            229..486
                     /locus_tag="O2701_002439"
     CDS             229..486
                     /locus_tag="O2701_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCZ4294309.1"
                     /translation="MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLE
                     DWKSIQETIYLESTGTMDKVREREKDNSGTTNIDDIDWDNL"
     gene            486..752
                     /locus_tag="O2701_002440"
     CDS             486..752
                     /locus_tag="O2701_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCZ4294310.1"
                     /translation="MSNYTVKIKNSAKSDLKKIKHSYLKKSFLEIVETLKNDPYKITQ
                     SFEKLEPKYLERYSRRINHQHRVVYTVDDRNKEVLILSAWSHYD"
     gene            1107..2183
                     /locus_tag="O2701_002441"
     CDS             1107..2183
                     /locus_tag="O2701_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCZ4294311.1"
                     /translation="MNQSVKLLKHLTDVNGIAGYEMQVKEAMRNYIEPVSDQIIEDNL
                     GGIFGKKNAENGQYSIMISGHMDEVGFMVTKIDKHGFISFTPVGGWWNQVMLSQKVTI
                     TTDSGKEIRGIIGSKPPHVLTPEERKKPMEIKNMFIDIGVSSKEEAEEAGVEVGNMVT
                     PYSEFEVLANDKYLTAKAFDNRYGCALAIEVLKRLKDENIGINLYSGATVQEEVGLRG
                     AKVAANTIKPDLAIAVDVGIAYDTPGMSGQTSDSKLGGGPVVIMMDATSIAHQGLRKH
                     IKDVAKEHNIEVQWDTTPGGGTDAGSIHVANEGIPTMTIGVTLRYMHSNVSVLNVDDY
                     ENSIRLVTEIVRSLNDESYKNIMW"
     gene            2431..3093
                     /locus_tag="O2701_002442"
     CDS             2431..3093
                     /locus_tag="O2701_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCZ4294312.1"
                     /translation="MLLEIKDLVYKASDRIILDHISLKVDKGESIAIIGPSGSGKSTF
                     QKQICNLFSPTSGELYFKGKPYNDYDPEELRQRISYLMQQSDLFGETIEDNMIFPSLA
                     RNDKFDRKRAKQLIKDVGLGHYQLSSEVENMSGGERQRIAIARQLMYTPDILLLDEST
                     SALDVNNKEKIENIIFKLADQGVAIMWITHSDDQSMRHFQKRITIVDGQISNTEELNQ
                     HE"
     gene            3086..3862
                     /gene="fetB"
                     /locus_tag="O2701_002443"
     CDS             3086..3862
                     /gene="fetB"
                     /locus_tag="O2701_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCZ4294313.1"
                     /translation="MSNTALGLTALLLVIPIIISYKEGLHIIKDLIVATLRAVVQLII
                     LGFLLHYIFKINDKWLLILCVLVIIINASWNTISRASPVMHHVFWISFLAIFIGTALP
                     LAGTIATGAIQFTANEVIPIGGMLANNGLIAINLAYQNLDRAFVQDGTNIESKLSLAA
                     TPKLASKGAIRESIRLAIVPTIDSVKTYGLVSIPGMMTGLIIGGVPPLQAIKFQLLVV
                     FIHTTATIMSALIATYLSYGQFFNARHQLVARNTDVKSES"
     gene            4258..5445
                     /locus_tag="O2701_002444"
     CDS             4258..5445
                     /locus_tag="O2701_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ4294314.1"
                     /translation="MSIMRLFTFILSIFIVGMVEMMVAGIMNLMSQDLHVSEAVVGQL
                     VTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFIIGNGIIAVAPNFSILVVGRIIS
                     SAAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYT
                     FLFLIIVSIIVGFLMMIYLPKDQEIQRGPVNHETPSHENHVTSKILRPAEVAKYLIIT
                     FLVLIANSVTFVFINPLILSNGHDMSFVSLALLVNGIAGVIGTSLGGIFSDKITSKRW
                     LMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQSGVIQHVEGDTSQV
                     MSWNMSSLNAGIGVGGIIGGLVMTHVSVQAITYTSAIIGALGLIVVFTLKNNHYAKTF
                     KSS"
     gene            complement(5507..6859)
                     /locus_tag="O2701_002445"
     CDS             complement(5507..6859)
                     /locus_tag="O2701_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HCZ4294315.1"
                     /translation="MKINTTGGQIHGITQDGLDIFLGIPYAEPPVHDNRFKHSTLKTQ
                     WSEPIDATEIQPIPPQPDNKLEDFFSSQSTTFTEHEDCLYLNIWKQHNDQTKKPVIIY
                     FYGGSFENGHGTAELYQPAHLVQNNDIIVITCNYRLGALGYLDWSYFNKDFHSNNGLS
                     DQINVIKWVHQFIESFGGDANNITLMGQSAGSMSILTLLKIPDIEPYFHKVVLLSGAL
                     RLDTLESARNKAQHFQKMMLDYLDTDDVTSLSTNDILMLMAKLKQSRGPSKGLDLIYA
                     PIKTDYIQNNYPTTKPIFACYTKDEGDIYITSEQKKLSPQRFIDIMELNDIPLKYEDV
                     QTAKQQSLAITHCYFKQPMKQFLQQLNIQDSNAQLWLAEFAWHDTSSAHYRSAYHILD
                     MVFWFGNLQILAAHQYPTTAHLKFLSRQMQNDLANFAKSGKMPWPMYHNERRYYRTYQ
                     "
BASE COUNT         2377 a         1007 c         1382 g         2339 t
ORIGIN      
        1 aaaattttaa aaatatttaa gcctgaaaat gataaagcgg tagggaacgt agtgtgttcc
       61 actgctgatt tctttttgag cattataggc ggtatgttgg aagaaaagaa caaaagtttg
      121 tgaacgatag catataggta ttaagaatac gatgaactcg ttggtaatat tgacgtactc
      181 aagtgcgtac gctataatta gcgtaacaga aaacaaagga gggtttatat gattatcact
      241 agccctacag aagcgagaaa agatttttat caattactaa aaaatgttaa taataatcac
      301 gaaccaattt atattagtgg caataatgcc gaaaataatg ctgtgattat aggtttagaa
      361 gattggaaaa gtatacaaga gacaatatat cttgaatcta ctggtacaat ggacaaagta
      421 agagaaagag aaaaagataa tagtggtaca acaaatatag atgatattga ttgggataat
      481 ctttaatgag caattacacg gttaagatta aaaattcagc gaaatcagat ttaaagaaaa
      541 taaaacattc ttatttaaag aagtcatttt tagaaattgt tgagacttta aaaaatgatc
      601 cgtataaaat aacacaatct tttgaaaaat tagagcctaa atatttagag cgatattcaa
      661 gaagaattaa ccatcagcac agggtcgtct ataccgtaga tgatcgaaat aaagaagtat
      721 taatactatc ggcatggtca cattatgatt aatgaatatt caatatctga ataactttaa
      781 tgataagtta attaaagaaa ctagtattta agtgtaggga aaatagcgac gttaatgcgt
      841 tgttatctct acacttttta attttataat agcgcaagac taaacagatt gaaattagta
      901 acaataaaag aataacgtat tataataagg aattttaaat tgtgactttt tcggaatatt
      961 aaattttaga aatatgaggt ttttaagcgg attcctcaca aaattttaaa aatatttaag
     1021 cctgaaaatg ataaagcggt agggaacgtt tttctgaaag ttagtgatac aatagtttta
     1081 agttgaaata caggaggatg aataacatga atcagtcagt caaattactt aaacatttaa
     1141 cagatgtaaa cggcattgct ggttatgaaa tgcaagttaa agaagcaatg cgtaactata
     1201 tagagcctgt cagtgatcaa attattgaag ataacttggg tggcattttt ggaaagaaaa
     1261 atgctgagaa tggtcaatac tcaattatga tttctggtca tatggatgaa gttggtttta
     1321 tggtaacaaa gattgataaa catggtttta tttcatttac gccagttggt ggatggtgga
     1381 atcaagtcat gctatctcaa aaagtaacga ttacaacaga ttcgggcaaa gaaattagag
     1441 gtatcatcgg ttctaaaccg ccacatgtct taacgcctga agaacgtaaa aagccaatgg
     1501 aaatcaaaaa tatgtttata gatattggtg ttagtagcaa ggaagaagct gaagaagctg
     1561 gcgttgaagt aggcaatatg gttacgccat atagtgaatt tgaagtgctt gcaaatgata
     1621 aatatttaac tgcgaaagca tttgataatc gctatggctg tgcattagct attgaggtat
     1681 taaaacgttt aaaagatgaa aatattggca ttaacttata cagtggtgcc acagtgcaag
     1741 aagaagttgg tttgcgtggt gcgaaagtgg cagcgaatac gattaaacca gacttggcga
     1801 tagctgtcga tgtaggtatt gcttatgata ccccaggtat gtcaggtcaa acgagcgata
     1861 gtaaactagg cggtggtcca gttgtcatta tgatggatgc tacaagtatt gctcaccaag
     1921 gtttgcgaaa gcatattaaa gatgtagcta aggaacataa catcgaagta caatgggata
     1981 cgacaccagg tggaggtaca gatgcgggaa gtattcatgt cgcaaatgaa ggtattccaa
     2041 cgatgacaat cggtgttacg ctgcgataca tgcattctaa tgtttcagtg ctcaatgtag
     2101 atgattatga aaattctatc cgtcttgtta ctgaaattgt ccgttcattg aatgatgaaa
     2161 gttataaaaa tatcatgtgg taatcaaatc cataaataat aaagaatcct tttaatatgg
     2221 taggttgtta aacaattgtc taattttaat tcttagtcat tagacagtat ccatgttaat
     2281 aggatttttt gtttttaatt taaatgctga aaatcaatta tgcctaaatt ttgatattac
     2341 aagaaaatga ttttttctta aatgtaattg cactaaaaac caaaaaaacg ggaataatat
     2401 acctgatata ttacatgagg agcggtgcaa atgttgttag aaattaaaga tttagtgtat
     2461 aaagcgagcg atagaatcat actagatcat atcagtctaa aagtagataa aggcgagagt
     2521 attgccatta taggtccatc aggtagtggt aaaagtacat ttcaaaagca aatatgtaat
     2581 ttgtttagtc caactagtgg agaactttat tttaaaggta aaccctataa tgattatgac
     2641 ccggaagaat tgcgtcaacg aatcagttat ttgatgcagc aaagtgactt gtttggtgaa
     2701 acgattgaag ataacatgat attcccatca cttgcacgta atgataaatt tgatagaaaa
     2761 cgtgcaaagc aattaattaa agatgtcggt ttgggacatt atcaattaag ttcggaagtg
     2821 gaaaatatgt cgggtggtga gcggcaaaga attgctatag cgcgccaact gatgtataca
     2881 ccggatattc ttttattaga tgaatcgacc agtgcattag acgttaataa taaagaaaag
     2941 atagaaaata tcatttttaa attagcagat caaggcgtgg caattatgtg gattacccac
     3001 agcgatgacc aaagtatgcg acactttcaa aagcgtataa caattgttga tggtcaaatt
     3061 tctaatacag aggagttgaa tcaacatgag taatacggca cttggattga cggcattgct
     3121 tttagtcatt ccgattatca tttcatataa agaaggttta catattatta aagatttaat
     3181 tgttgcgaca ttacgagcag ttgtgcaatt aatcattttg ggatttttgc tgcattatat
     3241 ttttaaaata aacgataaat ggctgcttat tttatgtgta ttggtcatta ttattaatgc
     3301 atcatggaat acaattagtc gagcatcacc agtgatgcat catgtgtttt ggatatcatt
     3361 tctagctatc ttcattggaa cggcattacc gcttgcaggt actattgcga caggggccat
     3421 tcaatttacc gcaaatgaag ttatacctat cggcggcatg cttgcaaata atggcttgat
     3481 tgcaattaat ttagcttacc agaatttaga tcgtgcattc gtacaagatg gtactaatat
     3541 tgaatctaaa ttatcacttg cagctacacc taaattggct tctaaaggtg caatacgtga
     3601 aagtattcgt ttagctatag tgccaactat tgattcggtt aaaacatatg ggcttgtgtc
     3661 gattcctggt atgatgacag gcttaattat tggtggcgta ccacctttac aagcgattaa
     3721 atttcaattg ttagtcgtgt ttattcatac aactgcgacc attatgtctg ctttgattgc
     3781 gacatattta agctatggtc aatttttcaa tgcaagacat caattagtag cacgaaatac
     3841 tgatgttaag agtgaatcat gatagatttt actgcatcag atttaggcat tagttttaat
     3901 tggaaatgaa gtgacgcgca catatagtat cgctattcat tagcgcagcg aaaatattca
     3961 taaaggcacg catactttgt agtcagttat ctgttctgac atataaagcg tgcgtgcttt
     4021 tttggagtta ttgttgaaac tgaagtaatt atacataatt attaaatgac atacttgtgt
     4081 taatttttca aatactgaaa aacaatttca ataattttcc aattaagcac agaaaattaa
     4141 agcaaaatat tatataatag aacggttata tataaaattg tgttgcacac attttttaat
     4201 aaatcgttat tctaagggaa atgaatatcg gaaattttgt ttgaaaggag ttttaaattg
     4261 tcaatcatgc gactatttac attcatttta agtattttta tcgtaggaat ggttgaaatg
     4321 atggttgcag gaattatgaa cttgatgagt caggacttac atgtatcaga agctgtcgtt
     4381 ggtcaattag tgacaatgta cgctttaaca tttgcgatat gtggacctat tctggttaaa
     4441 ttaacgaacc gtttttcatc aaggcctgta ttattatgga cattacttat atttatcatt
     4501 ggtaatggca ttattgctgt agcgccaaat ttttcaatat tagtagttgg tagaattatc
     4561 tcatctgcag cagcagcact aattatcgta aaagtattag ctattacagc gatgttatca
     4621 gcacctaaaa atcgtggtaa aatgattgga cttgtctata cagggtttag tggtgctaat
     4681 gtttttggtg taccaattgg aacggttatc ggcgatttag taggttggcg ctatacattt
     4741 ctattcttaa ttattgtgag tattattgtt ggcttcttga tgatgatcta tttaccgaag
     4801 gatcaggaaa tacaacgagg ccctgtgaat catgagacac catctcatga aaatcatgtt
     4861 acttcgaaaa tattaagacc tgctgaagta gcaaaatatt taattattac gtttttagta
     4921 ttgattgcaa actcagtgac attcgtcttt ataaatccac ttattttatc taatggtcat
     4981 gatatgtcat tcgtttcatt agcacttcta gtaaatggaa tcgctggcgt tattggaaca
     5041 tcattaggtg gtatattctc cgataaaatt acaagtaagc gttggttaat gatttctgtt
     5101 tctattttta tcgtcatgat gttacttatg aatttaatct tacctggttc aggtctattg
     5161 ttagcaggac tatttatttg gaatatcatg caatggagta ctaatccagc agtgcaaagc
     5221 ggtgtgattc aacatgttga aggcgacaca agccaagtaa tgagttggaa catgtctagt
     5281 ttaaacgctg gtattggtgt tggaggcatt attggaggct tggtcatgac acatgtttct
     5341 gttcaagcta tcacatatac gagtgccatc attggcgcat taggattaat cgttgttttc
     5401 acattgaaaa ataatcatta tgctaaaaca tttaaatcat cataattctc atatgaaaag
     5461 cacgcctgct atcaaattca ggtgtgcttt tttagatgcg ataacgttat tgatatgtgc
     5521 gataatagcg acgttcatta tgatacatcg gccaaggcat tttaccgctt ttagcaaaat
     5581 tagctaaatc attttgcatt tgtcgactta aaaatttaag gtgagcagtt gttggatatt
     5641 gatgtgctgc taatatttgt aagtttccga accaaaatac catatctaga atatggtaag
     5701 cactgcgata gtgtgcgcta gaagtatcat gccatgcaaa ttcagcaagc catagttgtg
     5761 cgtttgaatc ttgtatattg agttgttgta aaaattgctt catcggctgt ttgaaataac
     5821 aatgtgtaat cgctaaagat tgttgcttcg ccgtctgaac atcttcgtat tttaaaggaa
     5881 tatcatttaa ttccataatg tcgataaagc gttgcggcga taattttttc tgttcactag
     5941 taatataaat atcgccttca tcttttgtat aacatgcaaa aattggtttc gttgttggat
     6001 aattattttg tatataatct gttttaatag gcgcatatat taaatcaagc cctttagaag
     6061 gtcctcgaga ttgttttagc ttcgccatca gcataagaat atcatttgtc gataatgatg
     6121 taacatcatc tgtatctaaa taatcgagca tcattttttg gaaatgttgt gctttattgc
     6181 gtgcactctc aagggtgtct aatcgtagtg cgccacttag tagaacgact ttatggaagt
     6241 atggctcaat gtcaggtatt ttaagtaaag tcaaaatgct catactgcct gcagactgac
     6301 ccattaaagt aatgttatta gcgtcgccac cgaaggattc aataaattga tgcacccatt
     6361 ttatgacatt gatttgatct gaaaggccat tattggaatg aaaatcttta ttaaaatatg
     6421 accagtctaa atatcctaat gcgcctaaac gataattgca tgtaataacg ataatgtcgt
     6481 tattttgtac taaatgtgcc ggttgataga gttcggctgt accatgacca ttttcaaaac
     6541 taccaccata aaaataaatg atgacaggtt tcttcgtctg atcattatgt tgtttccaaa
     6601 tatttagata taaacagtct tcatgttcag taaaagttgt agattgtgag gagaaaaaat
     6661 cttctaattt gttgtctggt tgcggtggga tgggttgtat ttcagttgca tcaattggct
     6721 ctgaccattg tgtttttaac gtagaatgtt taaagcgatt gtcatgaact ggtggttctg
     6781 cataaggaat gcctaagaag atatctaaac catcttgtgt aataccatga atttgaccac
     6841 ctgtagtatt aatcttcata tttattaacc tcctttatca tccccaattt aatcatacca
     6901 cggtctatag atgataggtg acaagtataa caagacaatt ttgtgactga atcagacgag
     6961 cttgatatct gttttaactc aaaccatttt cagcattata tgatgtactt ctacgggagt
     7021 gggacagaaa tgataaagag ccacaaatga tttattatgt agtggttctt atacattagc
     7081 cacagctaat gtgtacttaa aaata
//
LOCUS       DALAGD010000121         6933 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.122, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000121 DALAGD010000000
VERSION     DALAGD010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6933
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.122"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(1..218)
                     /locus_tag="O2701_002446"
                     /pseudo
     CDS             complement(1..218)
                     /locus_tag="O2701_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500926.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(480..605)
                     /locus_tag="O2701_002447"
     CDS             complement(480..605)
                     /locus_tag="O2701_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294316.1"
                     /translation="MRYIHFSIITLITGIIMHITMYFVPFESRKMPLFMTIIFTI"
     gene            complement(825..1673)
                     /locus_tag="O2701_002448"
     CDS             complement(825..1673)
                     /locus_tag="O2701_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCZ4294317.1"
                     /translation="MNHIEISKDVKIPVLGFGVFQIPQEQTAEAVKEAIKAGYRHIDT
                     AQSYLNETEVGQGIEASGIDRSELFITTKVWIENVNYEDTIKSIERSLQRLNLDYLDL
                     VLIHQPYNDVYGSWRALEELKENGKIKAIGVSNFGVDRIVDLGIHNQIQPQVNQIEIN
                     PFHQQEEQVAALQQENVVVEAWAPFAEGKNQLFQNQLLQAIADKYNKSIAQVILRWLV
                     ERDIVVLAKSVNPERMAQNLDIFDFELTEEDKQQIATLEESNSQFFSHADPEMIKALT
                     SRELDV"
     gene            complement(1743..2159)
                     /locus_tag="O2701_002449"
     CDS             complement(1743..2159)
                     /locus_tag="O2701_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCZ4294318.1"
                     /translation="MKTKEVVALMNISQDTLRYYEKVGVIPPVNRDENGYRIYNDSDL
                     NWIYLVKNLRNAGVSIESLIEFCRLAQLPKNENIQAQQKQILNKQLEELNENLKTIHD
                     VRDLLQYKIDNYDNHIAKINASDNYDDNVERLWERK"
     gene            2403..2570
                     /locus_tag="O2701_002450"
     CDS             2403..2570
                     /locus_tag="O2701_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294319.1"
                     /translation="MRKIWVIDCLYASTFFVDCLTVDSHLYTLTNFEKLNHYLIIIFI
                     AHAQHKKETCQ"
     gene            2567..4990
                     /gene="hysA"
                     /locus_tag="O2701_002451"
     CDS             2567..4990
                     /gene="hysA"
                     /locus_tag="O2701_002451"
                     /EC_number="4.2.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase HysA"
                     /protein_id="HCZ4294320.1"
                     /translation="MTYRIKKWQKLSTITLLMAGVITLNGGEFRSVDKHQIAVADTNV
                     QTPDYEKLRNTWLDVNYGYDKYDENNPDMKKKFDATEKEATNLLKEMKTESGRKYLWS
                     GAETLETNSSHMTRTYRNIEKIAEAMRNPKTTLNTDENKKKVKDALEWLHKNAYGKEP
                     DKKVKELSENFTKTTGKNTNLNWWDYEIGTPKSLTNTLILLNDQFSNEEKKKFTAPIK
                     TFAPDSDKILSSVGKAELAKGGNLVDISKVKLLECIIEEDKDMMKKSIDSFNKVFTYV
                     QDSATGKERNGFYKDGSYIDHQDVPYTGAYGVVLLEGISQMMPMIKETPFNDKTQNDT
                     TLKSWIDDGFMPLIYKGEMMDLSRGRAISRENETSHSASATVMKSLLRLSDAMDDSTK
                     AKYKKIVKSSVESDSSYKQNDYLNSYSDIDKMKSLMTDNSISKNGLTQQLKIYNDMDR
                     VTYHNKDLDFAFGLSMTSKNVARYESINGENLKGWHTGAGMSYLYNSDVKHYHDNFWV
                     TADMKRLSGTTTLDNEILKDTDDKKSSKTFVGGTKVDDQHASIGMDFENQDKTLTAKK
                     SYFILNDKIVFLGTGIKSTDSSKNPVTTIENRKANGYTLYTDDKQTTNSDNQENNSVF
                     LESTDTKKNIGYHFLNKPKITVKKESHTGKWKEINKSQKDTQKTDEYYEVTQKHSNSD
                     NKYGYVLYPGLSKDVFKTKKDEVTVVKQEDDFHVVKDNESVWAGVNYSNSTQTFDINN
                     TKVEVKAKGMFILKKKDDNTYECSFYNPESTNSASDIESKISMTGYSITNKNTSTSNE
                     SGVHFELTK"
     gene            complement(5050..5904)
                     /locus_tag="O2701_002452"
     CDS             complement(5050..5904)
                     /locus_tag="O2701_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HCZ4294321.1"
                     /translation="MNKTNFGAYLNKSKSRKIYKIFDQNLKREFSYWQFRKLFKSYTR
                     QSDVHELKVCTQNDGTIEQIWIDEANQFGVSVTWKDNSIIGFVMRPLINYDAIKNETT
                     LKYAVPTTDCYEIFWGGDNELYNYHYLYPHQRYAIDLVIKENHKTYKNQGKVNTDYFC
                     FGKDIIAPANGTVEKVVNGVQDNSIGSTNESQFLGNYIVIKHAENEYSLIAHLHQYSI
                     IVNEGQNVKYGDIIGKVGNSGNSTEPHIHFQVMNDKNIEACTSLKIRFINNRELIKGD
                     VVCGLQAE"
     gene            complement(6115..6930)
                     /locus_tag="O2701_002453"
     CDS             complement(6115..6930)
                     /locus_tag="O2701_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HCZ4294322.1"
                     /translation="MSKRLLLFDFDETYFKHNTNEEDLSHLREMEKLLEKLTNNNEVI
                     TAVLTGSTFQSVMDKMDQVNMTFKPLHIFSDLSSKMFTWNNGEYVESETYKKKVLSEP
                     FLFEDIEDILRHISAQYNVEFIPQRAFEGNETHYNFYFHSTGNHNNDSRILEALVRYA
                     NDQNYTARFSRSNPLAGDPENAYDIDFTPSNAGKLYATQFLMRKYNIPVKSILGFGDS
                     GNDEAYLSYLEHAYLMSNSRDEALKQKFRLTKYPYYQGITLHVKEFVEGKYDY"
BASE COUNT         2370 a         1151 c          994 g         2418 t
ORIGIN      
        1 atatactaca tgaaccaaat ttatagaatc agaaggcgaa aacaaatctt caaaaatgtt
       61 tattgcatta tcatatacag ctaaaaataa gtattattga tagttttacc gtcctccaaa
      121 aactggtatt tatcgccact taaacttaaa tgaattgcgt tttttacctt tctaaaattc
      181 aactttttta tatccaaatt atttatatca aagcacataa tttacaacac cttattccct
      241 taagattttt ccttctttag ttaaagaagc tattcttttt cctttgttat ttattaactt
      301 ccaataagaa ccaccatgtg attgcccttt atttttggaa atagtccatt tagtttttga
      361 atctctccag gtaggatttt tcccttaaac cttttgagta aattcaccaa tactcatccc
      421 cttatcattt aacaaacttt tattaatgtt tttggttata ttatgagtct ttactgcttt
      481 tagattgtaa aaattatcgt cataaatagt ggcattttcc gagattcaaa tggtacaaag
      541 tacattgtaa tatgcataat gatacctgta attaatgtaa taatagaaaa gtgtatatat
      601 ctcatatcgc cacctgctat acaacttatt aaataattaa ttccaagaca tattaattat
      661 ctaattataa ccttagttac gttattacat aatataaaaa tatataataa cttatttgct
      721 tgctcctttc actttttaag agtaaaaaat aacctgaaac atagacttta acatccaagt
      781 ttcaggttag tgtaatgtaa atgatttact ttgttatatt atgattaaac atctaattct
      841 ctgcttgtaa gtgctttaat catctctgga tcagcatgtg agaaaaattg gctattactt
      901 tcttctaatg tcgcaatttg ttgtttatct tcttctgtaa gttcaaaatc aaatatatct
      961 aagttttgag ccatacgttc tggattcact gattttgcga gtacaacaat gtcacgttct
     1021 actaaccagc gtaaaataac ttgtgcaatt gatttgttgt atttatctgc aatggcctgc
     1081 aacaattgat tttggaataa ttgattttta ccttctgcaa atggtgccca agcttctacc
     1141 acaacatttt cttgttgaag tgctgcaact tgttcttctt gttgatggaa tggattaatt
     1201 tctatttggt tcacttgtgg ttgaatttga ttgtgaatac ctaaatctac aattcgatca
     1261 acgccgaagt tagatacacc gattgcttta attttaccat tttcttttaa ctcttctaat
     1321 gcacgccatg agccatatac gtcattgtaa ggttggtgaa taagcactag atcaagatag
     1381 tccaaattta atcgttgtaa tgatcgttcg attgatttga tagtatcttc atagttaaca
     1441 ttttcaatcc atactttagt cgtaatgaat agttcacttc tatcaatacc tgaagcttca
     1501 ataccttgac ctacttcagt ttcatttaaa taactttgtg ctgtatcaat atgtctataa
     1561 cccgctttaa ttgcttcttt aaccgcttct gctgtttgtt cttgaggaat ttgaaatact
     1621 ccgaaaccta atactggtat ctttacatct ttacttattt caatatgatt cataaaatga
     1681 acctccttta tcttgtatgt ccactataaa ccatggagcg aactctatag caagccatag
     1741 gtttactttc tctcccaaag acgttcaaca ttgtcatcat aattatcact agcattgatt
     1801 ttagcaatat gattatcata attatcaatt ttatattgta gtaaatctct cacatcatga
     1861 attgtcttta agttttcatt taattcttcg agttgcttat ttaaaatttg cttttgctgt
     1921 gcttgaatat tttcattttt aggaagctgc gctaacctgc aaaattcgat aagcgattca
     1981 atactgacgc ctgcatttcg caaatttttc actaaataaa tccaatttaa atcactatca
     2041 ttatatattc tatatccatt ttcatctcga ttaactggtg gaatcacacc aaccttttca
     2101 taatatctta aagtgtcttg agatatattc atgagcgcta cgacttcttt agttttcatt
     2161 gcgactatcc tttcagttat gtttggtcgt ctaaagtaat gttgctttat atattgtcat
     2221 cttcgtttga atacttctta ttttattact caaatttaaa tttgtctctt ttttaacatt
     2281 ttacatttca tcgtttttaa ttactttaaa aattgtataa cttaaatatt taaaatgata
     2341 taatcactaa gattgataat atttaatttt ttgaaaatta ttttagttgc aatttttggg
     2401 tgatgcggaa aatttgggtg atcgattgtt tatatgcatc aacattcttt gtggattgtt
     2461 tgacagtaga cagtcatttg tatacgttaa cgaattttga aaaattgaat cattatttaa
     2521 tcataatatt cattgcgcac gcgcaacaca aaaaggagac atgtcaatga catatagaat
     2581 aaagaaatgg caaaaattat ccaccattac gttattaatg gctggtgtga ttactttgaa
     2641 tggtggtgaa ttcagaagtg ttgataaaca tcaaatcgct gtggctgata cgaatgttca
     2701 aacgccagat tatgaaaaat tgaggaacac atggctggac gttaactatg gttatgataa
     2761 gtatgatgag aataatccag atatgaagaa gaagtttgat gctacagaga aagaggcgac
     2821 gaatttactc aaggaaatga aaactgaaag tggtaggaaa tacttgtggt caggagcgga
     2881 aacccttgaa actaattctt ctcatatgac tcgtacctat cgtaatattg agaaaatcgc
     2941 agaagcgatg aggaatccta aaaccacttt aaatactgac gaaaataaga agaaagtgaa
     3001 agatgccctt gagtggctgc ataaaaatgc atatggaaaa gaaccagata aaaaagtaaa
     3061 agaattaagt gagaatttta ctaaaacaac tggcaagaat accaacttaa attggtggga
     3121 ttatgaaatt ggaacaccta aatcattaac aaatacgctt atattgctga atgatcaatt
     3181 ttcaaatgaa gaaaagaaaa aattcactgc ccctattaaa actttcgccc cagatagtga
     3241 caaaatatta tcttctgtag gaaaagctga acttgctaaa ggcggaaatc tagtagacat
     3301 ttctaaggtg aaacttttag aatgtattat cgaagaagat aaagatatga tgaaaaagtc
     3361 tatagattca tttaataaag tcttcactta cgttcaagat tctgccactg gtaaagaacg
     3421 taatggattt tataaagatg gctcttacat tgatcatcaa gacgttccat acactggtgc
     3481 ttatggcgtt gtactcttag aggggatttc tcaaatgatg ccgatgataa aagaaacacc
     3541 ttttaatgat aaaacccaaa atgatacaac cttaaagtca tggattgacg acggatttat
     3601 gccactcatt tataaaggtg aaatgatgga tttatcacga ggtagagcta tcagtcgtga
     3661 aaatgaaacc agtcactcag catctgcaac agtaatgaaa tcattgttga gattaagtga
     3721 tgccatggat gattcaacaa aagctaagta taaaaagatt gtcaaatctt cagtagagtc
     3781 agattcaagt tataaacaaa atgattattt aaattcatat tcggacatag ataaaatgaa
     3841 gtctttaatg acagataaca gtatttctaa aaacggatta acacaacaac ttaaaatata
     3901 taatgacatg gatcgtgtca cctatcataa caaagactta gactttgcat ttggtttaag
     3961 tatgacgtcg aaaaacgtag cacgctatga aagtatcaac ggagagaatt taaaaggttg
     4021 gcacactggt gctggaatgt cttatttata taacagcgat gtcaaacact atcatgataa
     4081 cttctgggtg acagccgata tgaaacgttt atcaggtaca acaactttag acaatgaaat
     4141 attaaaagat acggatgata aaaagtcgag taaaactttt gttggcggaa caaaagttga
     4201 tgaccaacat gctagtatcg gaatggattt tgaaaatcag gacaaaactt taactgccaa
     4261 aaaatcatat ttcatattaa acgataaaat tgtcttctta ggaactggca ttaaaagtac
     4321 tgattcatca aagaatccag ttacaacgat tgaaaatcgc aaagcgaatg ggtatacgtt
     4381 atatacagac gataaacaaa caaccaattc tgataatcag gaaaacaatt cagtcttttt
     4441 agagtccaca gataccaaaa agaacatcgg ttatcatttt ttaaacaaac cgaaaataac
     4501 tgtaaaaaaa gaaagtcata ctggtaagtg gaaagaaata aataaaagtc aaaaggatac
     4561 acaaaaaact gatgagtatt atgaagtaac tcaaaagcat tctaattctg acaataaata
     4621 tggatatgtg ttgtatccag gcttatctaa agatgtcttt aagacaaaaa aagatgaagt
     4681 aactgtcgtt aagcaagaag atgacttcca cgttgtgaaa gataatgaat cggtttgggc
     4741 tggtgttaat tatagtaata gcactcaaac ttttgacatt aacaacacta aagtcgaagt
     4801 taaagccaaa ggtatgttta tacttaaaaa gaaagatgat aacacttatg aatgtagctt
     4861 ctataatcct gaatctacaa attccgcttc agatattgaa tctaaaattt caatgaccgg
     4921 ttactctatt acaaacaaaa atacgtcgac ttctaatgaa tccggcgtgc actttgaatt
     4981 aactaaataa gcaagaaaat ctcgacacta taccattttt atagtgtcga gattttataa
     5041 gtatcgccat cattcagctt gtaatccgca gaccacatcc cctttgataa gttctcgatt
     5101 atttataaat cgaattttta aagatgtaca tgcttcaata ttcttatcat tcattacttg
     5161 aaaatgtata tgaggttcag tagaattgcc agaattccca accttcccaa tgatatcacc
     5221 atatttaaca ttttgcccct cattcacaat gattgaatat tggtgtaaat gagctattaa
     5281 gctatactca ttttctgcgt gcttaattac aatataattt cctaaaaact gtgattcatt
     5341 cgtacttcca atactgttgt cttgaacccc attcactact ttttcaactg taccatttgc
     5401 tggcgcaatg atatcttttc caaaacaaaa ataatcagta tttacttttc cttgattctt
     5461 atatgtttta tgattttctt taataactaa atcaatagca tacctttgat gtgggtataa
     5521 atagtgataa ttatataatt cattatctcc accccaaaaa atctcatagc aatctgttgt
     5581 cggcactgcg tattttaaag tcgtttcatt cttaatcgca tcataattaa ttaacggacg
     5641 cataacaaat ccgataatac tattatcttt ccaagtcact gacactccaa attgatttgc
     5701 ttcatctatc catatttgct caatagtacc atcattttga gtacacactt ttaattcatg
     5761 tacatcagac tgccttgtat aagatttgaa aagtttccta aactgccaat aactaaactc
     5821 tcgcttcaaa ttttgatcaa aaatcttata tattttcctg ctcttacttt tatttaaata
     5881 agccccaaag tttgttttat tcatatatac ttaacctatt atattaattt tggtatcaat
     5941 tctttctcca gttcctgcgt catttttgcg ctacctgaat cgtctacact taattggata
     6001 aatcaacata taaataattt tttaaagcta aagtgtagtc gattcaccat attttctaat
     6061 catcccttaa atattaatat ttgcacttaa aaagtataca tatcactcaa ctaatcaata
     6121 atcatactta ccttcaacaa attctttaac atgcagtgtt atcccttgat aatatggata
     6181 ttttgttaat ctaaatttct gctttaatgc ttcatctcta ctattcgaca tcaaatatgc
     6241 atgttcaaga tagcttaaat atgcttcgtc attgccgcta tctccaaaac ctaatattga
     6301 ttttactggt atattatatt tcctcatcag aaattgagta gcatataatt ttccagcatt
     6361 tgacggtgtg aagtcaatat catacgcatt ttcaggatca ccagctaatg gattgcttcg
     6421 actaaatctc gctgtatagt tttggtcatt tgcgtatctg acaagtgctt ctaggatacg
     6481 actatcattg ttgtgattac ctgttgaatg aaaataaaaa ttataatgtg tttcattacc
     6541 ttcaaatgct ctttgtggaa taaattcgac attatattgc gcagaaatat gacgtaatat
     6601 atcttcaata tcttcaaata aaaatggttc gctcaagact ttctttttat atgtttctga
     6661 ttcaacatat tcgccattat tccaagtgaa cattttagaa ctcaaatcag aaaagatatg
     6721 taatggttta aacgtcatat taacttgatc cattttatcc atgacacttt gaaatgtact
     6781 tcctgtcaat acagcagtga tcacttcatt attattagtt agtttttcca acaatttttc
     6841 catttctctt aaatgactta aatcttcttc atttgtatta tgtttaaaat atgtttcatc
     6901 aaaatcaaac aataatagac gctttgacat act
//
LOCUS       DALAGD010000122         6717 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.124, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000122 DALAGD010000000
VERSION     DALAGD010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6717
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.124"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            170..1399
                     /locus_tag="O2701_002454"
     CDS             170..1399
                     /locus_tag="O2701_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HCZ4294323.1"
                     /translation="MSLRDEALEMHKRNQGKLEVKPNVKVTNKEELSLAYSPGVAEPC
                     KDIYEDKRKVYDYTIKGNTVAVITDGTAVLGLGNIGPEASIPVMEGKAVLFKSFAGIN
                     GVPIALNTTDTEEIIKTVKLLEPNYGGINLEDISAPRCFEIEERLKKETNIPVFHDDQ
                     HGTAIVTLAGLVNALRVVNKDIAKIKVVLNGAGAAGIAIVKLLYAYGVRNMVMCDSRG
                     AIFEGRSYGMNPTKDVVAKWTNKDKIEGSLEEVVKDADVFIGVSVANALSQDMVKSMA
                     DNPIIFAMANPNPEIIPDDAKAAGARVVGTGRSDYPNQINNVLAFPGIFRGALEVEAT
                     HINEEMKKAAVEAIADLIDSSELNEDYCIPGPFDKRVAPSVARNVAKAAMESGVARIE
                     VDPQDVYDKTMKLTDLQ"
     gene            1594..2451
                     /locus_tag="O2701_002455"
     CDS             1594..2451
                     /locus_tag="O2701_002455"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HCZ4294324.1"
                     /translation="MFKDFFNRTKKKKYLTVQDSKNNDVPAGIMTKCPKCKKIMYTKE
                     LAENLNVCFNCDHHIALTAYKRIEAISDEGSFTEFDKGMTSANPLDFPSYLEKIEKDQ
                     QKTGLKEAVVTGTAQLDGMKFGVAVMDSRFRMGSMGSVIGEKICRIIDYCTENRLPFI
                     LFSASGGARMQEGIISLMQMGKTSVSLKRHSDAGLLYISYLTHPTTGGVSASFASVGD
                     INLSEPKALIGFAGRRVIEQTINEKLPDDFQTAEFLLEHGQLDKVVHRNDMRQTLSEI
                     LKIHQEVTK"
     gene            2451..3395
                     /locus_tag="O2701_002456"
     CDS             2451..3395
                     /locus_tag="O2701_002456"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HCZ4294325.1"
                     /translation="MLDFEKPLFEIRNKIESLKESQDKNDVDLQEEIDMLEASLERET
                     KKIYTNLKPWDRVQIARLQERPTTLDYIPYIFDSFMELHGDRNFRDDPAMIGGIGFLN
                     GRAVTVIGQQRGKDTKDNIYRNFGMAHPEGYRKALRLMKQAEKFNRPIFTFIDTKGAY
                     PGKAAEERGQSESIATNLIEMASLKVPVIAIVIGEGGSGGALGIGIANKVLMLENSTY
                     SVISPEGAAALLWKDSNLAKIAAETMKITAHDIKQLGIIDDVISEPLGGAHKDIEQQA
                     LAIKSAFVAQLDSLESLSRDEIANDRFEKFRNIGSYIE"
     gene            3664..4632
                     /gene="pfkA"
                     /locus_tag="O2701_002457"
     CDS             3664..4632
                     /gene="pfkA"
                     /locus_tag="O2701_002457"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002462395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCZ4294326.1"
                     /translation="MKKIAVLTSGGDSPGMNAAVRAVVRTAIYNEIEVYGVYHGYQGL
                     LNDDIHKLELGSVGDTIQRGGTFLYSARCPEFKEQEVRKVAIENLRKRGIEGLVVIGG
                     DGSYRGAQRISEECKEIQTIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTAS
                     SHARTFIIEAMGRDCGDLALWAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHS
                     IVLVAEGCMTAQDCQKELSQYINVDNRVSVLGHVQRGGSPTGADRVLASRLGGYAVDL
                     LMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYELANKLSI"
     gene            4654..6411
                     /gene="pyk"
                     /locus_tag="O2701_002458"
     CDS             4654..6411
                     /gene="pyk"
                     /locus_tag="O2701_002458"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCZ4294327.1"
                     /translation="MRKTKIVCTIGPASESEEMIEKLINAGMNVARLNFSHGSHEEHK
                     GRIDTIRKVAKRLDKIVAILLDTKGPEIRTHNMKDGIIELERGNEVIVSMNEVEGTPE
                     KFSVTYENLINDVQVGSYILLDDGLIELQVKDIDHAKKEVKCDILNSGELKNKKGVNL
                     PGVRVSLPGITEKDAEDIRFGIKENVDFIAASFVRRPSDVLEIREILEEQKANISVFP
                     KIENQEGIDNIAEILEVSDGLMVARGDMGVEIPPEKVPMVQKDLIRQCNKLGKPVITA
                     TQMLDSMQRNPRATRAEASDVANAIYDGTDAVMLSGETAAGLYPEEAVKTMRNIAVSA
                     EAAQDYKKLLSDRTKLVETSLVNAIGISVAHTALNLNVKAIVAATESGSTARTISKYR
                     PHSDIIAVTPSEETARQCSIVWGVQPVVKKGRKSTDALLNNAVATAVETGRVSNGDLI
                     IITAGVPTGETGTTNMMKIHLVGDEIANGQGIGRGSVVGTTLVAETVKDLEGKDLSDK
                     VIVTNSIDETFVPYVEKALGLITEENGITSPSAIVGLEKGIPTVVGVEKAVKNISNNM
                     LVTIDAAQGKIFEGYANVL"
BASE COUNT         2378 a          898 c         1362 g         2079 t
ORIGIN      
        1 tgtatcttta taaatattat tttattatcg tttatcgcta tttgtgttac ataatcaaaa
       61 ccataaattc ttactcattc agatttaccc aatattttta cttttataat gtaatgcgtt
      121 ttatccaagt tattttttaa aaataaatat tgaattaggg gctgatttca tgtcattaag
      181 agatgaagca ttggaaatgc acaaacgtaa tcaaggtaaa ttagaagtta aaccaaatgt
      241 aaaagttact aataaagagg aattaagttt agcatactca cctggcgttg ctgaaccgtg
      301 taaagatatt tatgaagata aaagaaaagt atatgattac acaattaaag gaaatacagt
      361 tgcagttatt actgatggaa cagcggtatt aggtttaggt aacattggac ctgaagcaag
      421 tattcctgta atggaaggta aagcagtatt attcaaaagc ttcgctggta tcaatggggt
      481 gcctattgcg ttaaatacaa ctgataccga agaaatcatt aaaacagtta agttgttaga
      541 acctaattat ggtggtatta atttagagga tatttcggca ccacgttgtt ttgaaattga
      601 agaacgattg aaaaaagaaa ctaatattcc ggtattccat gacgatcaac atggtacagc
      661 aattgtaaca ttggcaggtt tggtaaatgc attgagagtt gttaacaaag atattgctaa
      721 aataaaagtt gtactaaatg gtgctggtgc agcaggaata gccattgtta aattactata
      781 cgcgtatggt gtaagaaata tggttatgtg tgactcaaga ggcgcaattt ttgaaggacg
      841 ttcatatggt atgaatccta cgaaagatgt tgtagcaaaa tggacaaata aagataagat
      901 tgaagggtct ttagaagaag tcgtaaaaga tgcagatgta tttatcgggg tttctgtagc
      961 taatgcgctg tcacaagata tggttaagag tatggcagat aatccaatta tatttgcaat
     1021 ggctaatcca aatcctgaaa taatacctga tgatgccaaa gcggcaggtg cacgagttgt
     1081 tggtacagga cgttcagact atcctaacca aattaataat gtattagctt tccctggtat
     1141 ttttagaggt gcattagagg ttgaagctac acatataaat gaagaaatga aaaaggcagc
     1201 tgtagaagcg attgctgatt taatcgatag ttctgaatta aatgaagact actgtatccc
     1261 aggaccgttt gataaacgtg tagcgccatc agttgctcgt aatgttgcta aagcggcaat
     1321 ggaatctgga gtagctagga ttgaagttga tccgcaagat gtgtatgata aaacaatgaa
     1381 acttacagat ttacaataaa aataattaac gtttaattaa attgcttaaa agttagcaat
     1441 aaagagtcat taaaatgatg aaaatgaaag tttatgataa acattcaaca gtcaaacgaa
     1501 tataaatcaa ataaatttaa acccgttttt aactggtcaa gttcagttta aggctctaaa
     1561 tggttagaac agaggttatt tggaggtttt cctatgttta aagatttttt taatcgaaca
     1621 aagaaaaaga aatatcttac agtacaagac tctaaaaata atgatgtgcc tgcaggtatt
     1681 atgactaagt gtccaaagtg taagaaaatt atgtacacaa aagaattagc tgaaaattta
     1741 aatgtgtgct ttaattgtga tcatcatatt gctttaactg cgtataaacg tatagaagca
     1801 atttctgatg aaggatcatt tacagaattc gataagggaa tgacctctgc gaatccatta
     1861 gattttccaa gttatttaga aaaaattgaa aaggaccaac aaaagacagg tcttaaagaa
     1921 gcagttgtga ctggtacagc acaactagat ggtatgaaat ttggcgttgc tgtcatggat
     1981 tcacgtttta gaatgggaag tatgggatcg gttatcggtg aaaagatatg tcgcatcatt
     2041 gattactgca ctgagaaccg tttaccattt attcttttct ctgcaagtgg tggtgcacgt
     2101 atgcaagaag gtattatttc cttgatgcaa atgggtaaaa ccagtgtatc tttaaaacgt
     2161 cattctgacg ctggactatt atatatatca tatttaacac atccaactac tggtggtgta
     2221 tctgcaagtt ttgcatcagt tggtgatata aatttaagtg agccaaaagc gttgataggt
     2281 tttgcaggtc gtcgagttat tgaacagaca ataaacgaaa aattgccaga tgatttccaa
     2341 actgcagaat ttttattaga gcatggacaa ttggataaag ttgtacatcg taatgatatg
     2401 cgtcaaacat tgtctgaaat tctaaaaatc catcaagagg tgactaaata atgttagatt
     2461 ttgaaaaacc actttttgaa attcgaaata aaattgaatc tttaaaagaa tctcaagata
     2521 aaaatgatgt ggatttacaa gaagaaattg acatgcttga agcgtcattg gaacgagaaa
     2581 ctaaaaaaat atatacaaat ctaaaaccat gggatcgtgt gcaaattgcg cgtttgcaag
     2641 aaagacctac gaccctagat tatattccat atatctttga ttcgtttatg gaactacatg
     2701 gtgatcgtaa ttttagagat gatccagcaa tgattggtgg tattggcttt ttaaatggtc
     2761 gtgctgttac agttattgga caacaacgtg gaaaagatac aaaagataat atttatcgaa
     2821 attttggtat ggcgcatcca gaaggttatc gaaaagcatt acgtttaatg aaacaagctg
     2881 aaaaattcaa tcgtcctatc tttacattta tagatacaaa aggtgcatat cctggtaaag
     2941 ctgctgaaga acgtggacaa agtgaatcta tcgcaacaaa tttgattgag atggcttcat
     3001 taaaagtacc agttattgcg attgtcattg gtgaaggtgg cagtggaggt gctctaggta
     3061 ttggtattgc caataaagta ttgatgttag agaatagtac ttactctgtt atatctcctg
     3121 aaggtgcagc ggcattatta tggaaagaca gtaatttggc taaaattgca gctgaaacaa
     3181 tgaaaattac tgcccatgat attaagcaat taggtattat agatgatgtc atttctgaac
     3241 cacttggcgg tgcacataaa gatattgaac agcaagcttt agctattaaa tcagcgtttg
     3301 ttgcacagtt agattcactt gagtcattat cacgtgatga aattgctaat gatcgctttg
     3361 aaaaattcag aaatatcggt tcttatatag aataatcaac ttgagcattt ttatgttaaa
     3421 tcgatactgg gttttaccat aaattgaagt acattaaaac aataatttaa tatttagata
     3481 ctgaatttta actaagatta gtagtcaaaa ttgtggctac taatcttttt ttaattaagt
     3541 taaaataaaa ttcaatattt aaaacgttta catcaattca atacattagt tttgatggaa
     3601 tgacatatca atttgtggta atttagagtt aaagataaat cagttataga aaggtatgtc
     3661 gtcatgaaga aaattgcagt tttaactagt ggtggagatt cacctggaat gaatgctgcc
     3721 gtaagagcag ttgttcgtac agcaatttac aatgaaattg aagtttatgg tgtgtatcat
     3781 ggttaccaag gattgttaaa tgatgatatt cataaacttg aattaggatc agttggggat
     3841 acgattcagc gtggaggtac attcttgtat tcagcaagat gtccagagtt taaggagcaa
     3901 gaagtacgta aagttgcaat cgaaaactta cgtaaaagag ggattgaggg ccttgtagtt
     3961 attggtggtg acggtagtta tcgcggtgca caacgcatca gtgaggaatg taaagaaatt
     4021 caaactatcg gtattcctgg tacgattgac aatgatatca atggtactga ttttacaatt
     4081 ggatttgaca cagcattaaa tacgattatt ggcttagtcg acaaaattag agatactgcg
     4141 tcaagtcacg cacgaacatt tatcattgaa gcaatgggcc gtgattgtgg agatctagca
     4201 ttatgggctg gattatcagt tggtgctgag acaattgtag ttccagaagt gaaaacagat
     4261 attaaagaaa tagctgataa aattgaacaa ggtattaaac gtggtaagaa acactcaatc
     4321 gttcttgtag cagaaggttg tatgactgcg caagattgtc aaaaagaatt atcacaatac
     4381 atcaatgttg ataatagagt gtctgtgtta ggtcacgttc aacgtggtgg tagcccaaca
     4441 ggtgcggata gagttttagc atcacgttta ggtggatatg cggtagactt attaatgcaa
     4501 ggtgaaacag ctaagggtgt tggaattaag aacaataaaa ttgtagcaac atcttttgat
     4561 gaaatttttg atggtaaaga tcataaattt gattatagtc tatatgaact tgctaacaag
     4621 ttatctatat aagatttcag gaggaattat aaaatgagaa aaactaaaat tgtatgtaca
     4681 attggaccag cttcagaatc agaagaaatg attgagaaat taatcaatgc tggtatgaac
     4741 gttgcacgat taaacttttc acatggtagt catgaagagc ataaaggtag aattgataca
     4801 attcgtaaag tagctaaaag attagacaaa attgtagcaa ttttattaga tacaaaaggt
     4861 ccagaaattc gtacgcataa tatgaaagac ggtatcattg aacttgaacg tggcaacgaa
     4921 gttattgtta gcatgaatga agttgaagga acacctgaaa agttctcagt aacatatgaa
     4981 aacttaatta acgatgttca agtaggttca tacattttac ttgatgatgg cttaattgaa
     5041 ttacaagtta aagatattga ccatgctaaa aaagaagtta aatgtgatat tttaaactct
     5101 ggtgagctta aaaacaaaaa aggtgttaac ttacctggcg taagagtaag tttacctggt
     5161 attacagaaa aagatgctga agatatccgt ttcggtatta aagaaaatgt tgacttcatt
     5221 gcagcaagtt tcgtacgtcg tcctagtgat gttttagaaa ttcgtgaaat tttagaagaa
     5281 caaaaagcta acatttcagt attccctaaa attgaaaacc aagaaggtat tgataatatt
     5341 gcggaaattc ttgaagtgtc tgatggttta atggttgcac gtggtgacat gggtgttgaa
     5401 attccacctg aaaaagtacc aatggttcaa aaagatttaa tcagacaatg taacaaatta
     5461 ggtaaaccag ttattacagc tacacaaatg ttagattcta tgcaacgtaa cccacgtgct
     5521 acacgtgcag aagctagtga cgttgccaac gcaatctatg atggtacaga tgcagtaatg
     5581 ttatctggtg aaactgctgc tggtttatat cctgaagaag ctgttaaaac aatgagaaat
     5641 attgctgtat cagctgaagc agcccaagat tacaaaaagt tattgtcaga tcgtactaaa
     5701 ttagttgaaa cttcattagt gaatgctatc ggtatttcgg ttgcacatac agctttaaac
     5761 ttaaatgtta aagcaattgt agctgctact gaaagtggtt caacggcacg tactatctcc
     5821 aaatatcgtc cacattcaga cattattgcg gtgactccaa gtgaagaaac tgcacgtcaa
     5881 tgttcaattg tttggggagt tcaacctgta gttaaaaaag gacgtaagag tacagatgca
     5941 ttgttaaaca atgcagttgc aacagctgtt gaaactggta gagtatctaa tggtgattta
     6001 atcattatta ctgctggtgt accaactggt gaaactggaa ctactaatat gatgaaaatc
     6061 cacctagttg gtgacgaaat tgctaatggt caaggtattg gacgtggatc agttgttggt
     6121 actacgttag ttgctgaaac tgttaaagat ttagaaggta aagatttatc tgacaaagtt
     6181 atcgttacta actcaatcga tgaaacgttt gtaccttatg tagaaaaagc tttaggctta
     6241 attacagaag aaaatggtat tacatcacca agtgcaattg ttggtttaga aaaaggtatt
     6301 ccaacagttg taggtgtaga aaaagctgtt aaaaacataa gcaataacat gttagttacg
     6361 attgatgctg ctcaaggtaa aatctttgaa ggatatgcaa acgtactata atttataaaa
     6421 aaacgtcttt ccatttatca acaatggaaa ggcgtttttt ggttcatctg gtattttatg
     6481 acgtaattaa taggttattt gataatgata gtgtatgaat ggcaatctat ataaatgttt
     6541 atatctttta tacatgtaca ttatcacctt caaactttca ctcatattac tttggaaatt
     6601 tattataaaa tagaagtatg gatgtatttc tgaaatgata cattattaaa tagatgagaa
     6661 agtaaaagtt ttgagccaag tacgcaattt aatattataa gttgcatata aaacagg
//
LOCUS       DALAGD010000123         6593 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.126, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000123 DALAGD010000000
VERSION     DALAGD010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6593
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.126"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            50..325
                     /locus_tag="O2701_002459"
     CDS             50..325
                     /locus_tag="O2701_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294328.1"
                     /translation="MTKETDNKKTVATSDNKDTQQQNQPEEDERQGLNGYRKTDLDLE
                     IEQELREMMKTGENETKNDYKKFKVFSLISTLVIVILAIIRFVHKMM"
     gene            complement(511..1233)
                     /locus_tag="O2701_002460"
     CDS             complement(511..1233)
                     /locus_tag="O2701_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCZ4294329.1"
                     /translation="MINAEPIISKMKNQKINYDKVLKKLIGQWEREAIRPKILLHSCC
                     APCSTYTLEFLTQYADIAIYFANSNIHPKNEYLRRAKVQEQFVEDFNRKTGANVKYIE
                     APYEPHKFVKMVKDKELADEKEGGLRCTACFEMRLDIVAKAAVEHGYDYFGSAITLSP
                     KKNAQLINELGMDVQKIYDVNYLPSDFKKSKGYERSIEMCNDYNIFRQCYCGCVFAAM
                     QQGIDFKTVNKEAKAFLEQYPD"
     gene            1587..2036
                     /locus_tag="O2701_002461"
     CDS             1587..2036
                     /locus_tag="O2701_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HCZ4294330.1"
                     /translation="MTALFPYIAFENSKEALAYYEEVFGATDVKRLEVGEEQASHFGM
                     TKEEAQEATMHAEFEVLGVKVLCSDSFGRADKINNGISLLIDYDVNNKEDADKVEAFY
                     EQIKDHSSIEIELPFADQFWGGKMGVFTDKYGVRWMLHGQDYTAIQQ"
     gene            complement(2117..2704)
                     /locus_tag="O2701_002462"
     CDS             complement(2117..2704)
                     /locus_tag="O2701_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCZ4294331.1"
                     /translation="MTIDRRIRKSQVAMRNAFIELLHQHQLEEITVQQIADLADVNRS
                     TFYTHYYDKYDLLEKLEDQQLEEIRDFIHDEKLNGAVKLSTDNIHQIMTVLIEMIEKD
                     IAFYQLMFRMGKDSNIHEKLYDLIMCHLQRYKNENDQINGIPFSYFMSYVSGAGLSFI
                     RHWVEDPNRIDKVDLIRHFNTIVNQGPATIIKNIQ"
     gene            complement(2682..2780)
                     /locus_tag="O2701_002463"
     CDS             complement(2682..2780)
                     /locus_tag="O2701_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294332.1"
                     /translation="MPIINVGDLWDNEHNVYNHWGGLDINDYRSTY"
     gene            2837..4519
                     /locus_tag="O2701_002464"
     CDS             2837..4519
                     /locus_tag="O2701_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HCZ4294333.1"
                     /translation="MNQHLLGNPKLTVTHVNEVKAGINHIVVDSVQYGNQEMIMEKDV
                     TVEMRDGEKLYINIFRPNKDGKFPVVMSADTYGKDNKPKITNMGALWPTLGTIPTSSF
                     TPEESPDPGFWVPNDYVVVKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAANQSWSN
                     GNIGTNGVSYLAVTQWWVASLNPPHLKAMIPWEGLNDMYREVAFHGGIPDTGFYRFWT
                     QGIFARWTDNPNIEDLIQAQQEHPLFDDFWKQRQVPLSQIKTPLLTCASWSTQGLHNR
                     GSFEGFKQAASEEKWLYVHGRKEWESYYARENLERQKSFFDFYLKEENNDWKDTPHVI
                     YEVRDQFYKGEFKSASAFPLPNAEYTPLYLNAENHTLNHAKISSAHVAQYDSEDKQQD
                     VSFKYTFDKDTELVGNMNLKLWVSTKDSDDMDLFAGIKKLDRRGNEVNFPDFNHIENG
                     QVATGWLRVSHRELDQEKSSIAQPWHKHETELKLSQDEIVPVEIELLPSGTLFKQGET
                     LEVVVKGSEIVIGNSTPGMKTRYEHEETVNKGMHMIYTGGKYDSQLIIPIVN"
     gene            complement(4648..5031)
                     /locus_tag="O2701_002465"
     CDS             complement(4648..5031)
                     /locus_tag="O2701_002465"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015901315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCZ4294334.1"
                     /translation="MIRTMMNAKIHRARVTESNLNYVGSITIDSDILEAVDILPNEKV
                     AIVNNNNGARFETYVIAGERGSGKICLNGAASRLVEVGDVVIIMTYAQLNEEEIKNHA
                     PKVAVMNEDNVIIEMIHEKENTIVL"
     gene            complement(5033..5884)
                     /locus_tag="O2701_002466"
     CDS             complement(5033..5884)
                     /locus_tag="O2701_002466"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCZ4294335.1"
                     /translation="MTKLITTVKEMQHIVKAAKRSGTTIGFIPTMGALHDGHLTMVRE
                     SVSTNDITIVSVFVNPLQFGPNEDFDAYPRQIDKDLELVSEVGADIVFHPAVEDMYPG
                     ELGIDVKVGPLADVLEGAKRPGHFDGVVTVVNKLFNIVMPDYAYFGKKDAQQLAIVEQ
                     MVKDFNHAVEIIGIDIVREADGLAKSSRNVYLTEQERQEAVHLSKSLLLAQALYQDGE
                     RQSKVIIDRVTEYLESHISGRIEEVAVYSYPQLVEQHEITGRIFISLAVKFSKARLID
                     NIIIGAE"
     gene            complement(5877..>6593)
                     /gene="panB"
                     /locus_tag="O2701_002467"
     CDS             complement(5877..>6593)
                     /gene="panB"
                     /locus_tag="O2701_002467"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCZ4294336.1"
                     /translation="GIDMILVGDSLGMTVLGYESTVQVTLADMIHHGRAVRRGAPNTF
                     VVVDMPIGAVGISMTQDLNHALKLYQETNANAIKAEGAHITPFIEKATAIGIPVVAHL
                     GLTPQSVGVMGYKLQGATKEAAEQLILDAKNVEQAGAVALVLEAIPNDLAEEISKHLT
                     IPVIGIGAGKGTDGQVLVYHDMLNYGVEHKAKFVKQFADFSVGVDGLKQYDQEVKSGA
                     FPSEEYTYKKKIMNEVNNND"
BASE COUNT         2130 a         1216 c         1035 g         2212 t
ORIGIN      
        1 aaatatcaca tatcattaaa tatagcgttt tataacggag gcgtttgtta tgactaaaga
       61 aacagataat aaaaaaacag tagcgacttc tgacaacaaa gacacacagc aacaaaatca
      121 acctgaagaa gatgaacgtc aaggtttgaa tggttatcgt aaaacagatc ttgatttaga
      181 aatcgagcaa gagctacgtg aaatgatgaa aacaggagaa aatgaaacga aaaatgacta
      241 taaaaagttt aaagtgtttt cacttatttc aacacttgtc attgtcattt tagcaattat
      301 aagatttgtt cataaaatga tgtaattaga gtgagacatt gttttatgtc tcaggatcca
      361 gttattcatt atatctacaa tatttacgat tatataaata acccgagatt ttagtatgat
      421 tcatttcact aaaatctcgg gtttctattt gataattttt aatgggatat ggcatgtata
      481 cgttcttgcc ttttatctca tttccaatga ttaatctgga tattgttcta aaaatgcttt
      541 cgcttcttta ttaactgttt taaaatcaat accttgttgc atcgctgcaa agacacatcc
      601 acaataacac tgcctaaaga tattatagtc attacacatt tctatggaac gctcataacc
      661 tttacttttc ttaaaatcac ttggcaaata gttcacatcg tatatttttt ggacatccat
      721 accaagttca ttgattaatt gtgcgttctt tttaggtgat aatgtgattg cactgccaaa
      781 ataatcatag ccatgttcta cagctgcttt cgctacaata tccaaacgca tttcaaaaca
      841 agcggtacaa cgtaagccgc cttctttttc atcagctaat tctttatcct tcaccatttt
      901 cacaaactta tgcggttcat aaggtgcttc aatatacttc acattcgcac cagtcttgcg
      961 attaaaatct tccacaaatt gttcttgcac tttagcacgt cgtaagtact catttttcgg
     1021 atgaatattt gaattcgcga aataaattgc aatgtctgca tattgtgtta aaaactctaa
     1081 tgtatatgta ctacaaggtg cacaacaact atgcaataag attttaggtc tgattgcttc
     1141 tctttcccac tgcccgatta atttcttcaa caccttgtca taattaattt tttgattttt
     1201 cattttactg ataataggtt cagcattaat catgatttaa cctcccacat ttaatcatta
     1261 acttctatta tatatgattc atattaaatg tcagtcaaaa aagttagaaa ttcattttaa
     1321 tgcatttata tttcgaaaat cccttatgta tctaaaagca ttttttaaac ttgaatttta
     1381 aaacactaaa caacacatac gtctctgtgt cattttcatt ttttgtatgt catatatatg
     1441 tttacttcat ttaaatcaat ttcatcttat aatttatcgt gtattttaca aaagattgac
     1501 ttcaattcat cgtaaaagtt atacttttgc cattttttaa tgtaacatgg tgttagtaat
     1561 aaaaataata cattgaggtg ttttacatga cagcattatt cccttatatc gcttttgaaa
     1621 attcaaaaga agcccttgca tattacgaag aagtatttgg tgcaactgac gttaaacgtt
     1681 tagaagttgg cgaagaacaa gcgtcacatt ttggtatgac taaggaagaa gcgcaagaag
     1741 caactatgca tgctgaattt gaagtgcttg gcgtaaaagt gttatgttct gattcttttg
     1801 gtcgcgctga caaaattaat aatggcatat cattattaat tgattatgat gttaacaata
     1861 aggaagatgc tgataaagtt gaagcattct atgagcaaat taaagatcat tcttcaattg
     1921 aaatagaatt accgtttgct gaccaattct ggggtggcaa aatgggcgtc tttaccgata
     1981 aatacggtgt tcgttggatg ttacatggtc aagactatac tgcaatccaa caataatgtt
     2041 attgataaag atatcattta ataagcttat gttatttagc aaggcaagcc actctcgatt
     2101 catggcttgc cttattttat tgtatatttt taattatcgt tgctggccct tgattgacaa
     2161 tcgtattaaa atgccttatt aagtcaactt tgtctatacg gtttggatct tctacccaat
     2221 gtcttataaa agacaatccc gcacctgaaa cataactcat gaaataagaa aatggtatac
     2281 cattaatttg atcattttca tttttataac gttgtaaatg acacataatt aaatcataaa
     2341 gtttttcgtg gatatttgaa tctttaccca tacgaaacat caattgataa aatgcgatgt
     2401 ctttttctat catttctatt aaaacggtca taatttgatg tatgttatcc gtggataact
     2461 taactgctcc atttaacttc tcatcatgaa tgaagtctct tatttcctcc aactgctggt
     2521 cctctaattt ttcaagcaaa tcatacttat cataataatg cgtataaaat gtactacggt
     2581 taacatcagc taaatctgca atttgttgca cagtaatctc ttctaattgg tgttgatgta
     2641 aaagttcaat aaatgcattt ctcattgcaa cttgtgattt tctaatacgt cgatctatag
     2701 tcatttatat caagtcctcc ccaatgatta taaacgttat gttcattatc ccacaaatct
     2761 ccaacattga tgattggcac acaatgttta cctgtttaat ataggtgata caaacaaaca
     2821 gaaaaaggtg ataacaatga accaacattt actaggaaat ccaaaattaa ctgtaactca
     2881 tgtcaatgaa gttaaagccg gtattaacca catcgttgtc gacagtgttc aatatggaaa
     2941 tcaagaaatg attatggaaa aagatgtcac tgtggaaatg cgcgatggcg aaaaattata
     3001 tattaatatt ttcagaccaa ataaagatgg caaattccct gtagttatgt ctgcagatac
     3061 ttacggtaaa gataataagc ctaaaatcac aaatatgggt gccctttggc caacattagg
     3121 taccattccg acatctagtt ttacacctga agaatcacca gacccaggat tttgggtgcc
     3181 aaatgattat gttgtagtta aagttgcatt acgcggtagt gacaaatcca aaggcgtctt
     3241 atctccatgg tcaaaaagag aagcggaaga ttattacgaa gtgattgaat gggcagcaaa
     3301 tcagtcatgg agtaatggaa atatcgggac aaatggtgtt tcttatcttg cggtgactca
     3361 atggtgggtc gcatcattaa atccaccaca tttaaaagca atgattcctt gggaaggctt
     3421 aaatgatatg tatagagaag tagcctttca cggaggtata ccagatactg gcttttatcg
     3481 tttctggact caaggtattt ttgcgagatg gacagataat ccaaatatcg aagatttgat
     3541 tcaagcacaa caagaacatc ctctgttcga tgatttttgg aaacagcgtc aagtgccatt
     3601 atcacaaatt aaaacacctc tactaacatg tgctagttgg tctacacaag gtttgcacaa
     3661 ccgtggctct tttgaaggat ttaaacaagc tgcatctgaa gaaaaatggc tatatgtgca
     3721 tggacgtaaa gagtgggaaa gttactacgc tagagaaaat ctcgaacgcc aaaaatcatt
     3781 ctttgatttt taccttaaag aagaaaataa cgattggaaa gatacgcctc atgtcattta
     3841 tgaagttaga gatcaatttt ataaaggcga attcaaatca gcgtcagcgt tccctttacc
     3901 taacgcagaa tatacaccat tgtatttgaa tgctgaaaat cacacattga atcatgcaaa
     3961 gattagtagc gcgcatgtcg cacaatatga ctctgaagat aaacaacaag atgtaagttt
     4021 taaatatacg tttgacaaag atactgagtt agttggaaac atgaacttaa aactatgggt
     4081 aagcactaaa gactcagatg atatggattt atttgcaggt attaaaaagt tagatcgtcg
     4141 tggtaatgaa gttaacttcc ctgattttaa tcatattgaa aatggtcaag tagctactgg
     4201 ttggttacgc gtatcacatc gtgaattaga tcaagaaaaa tcctcaatcg cgcaaccttg
     4261 gcataaacat gaaacagaat taaagttgtc acaagatgag attgtacctg ttgaaatcga
     4321 attgttacct tcaggcacgc tatttaaaca aggcgaaaca ttggaagttg ttgtaaaggg
     4381 tagtgaaatt gtaattggta atagtactcc tggcatgaaa acacgttatg aacatgaaga
     4441 aaccgtaaat aaaggcatgc acatgattta tactggtggt aaatatgatt cacaattaat
     4501 cattcctatc gttaattgat atgcagcaat tacggtcgct tttgattaaa agtgacatag
     4561 tgataggact gtataacaag agaaagccac acgcttggaa tcttaaacca aggtgtggcc
     4621 ctttttatta ttgatggcta tttaatttta taacactatc gtattttctt tttcatgaat
     4681 catttcaata atgacattat cttcattcat tactgctact ttaggtgcat ggtttttaat
     4741 ttcttcttca ttcaactgtg cataagtcat gattatgact acatcgccta cttcaacaag
     4801 tcttgacgct gcaccgttta aacaaatttt accactacct ctttcaccag ctattacgta
     4861 tgtttcaaaa cgtgcaccat tattattatt cacgatggct actttttcat ttggcaagat
     4921 gtctaccgct tccaatatat ctgaatcaat cgtaatgcta cctacataat ttaaatttga
     4981 ctcagtcact cttgctctat gaattttagc attcatcatt gttcttatca ctttattcag
     5041 ctccaattat tatattatct attaaacgcg cttttgaaaa tttaacagct aacgagataa
     5101 atatgcgtcc agttatttcg tgttgttcta ctaattgagg ataactataa acagcaactt
     5161 cttcaatgcg tccacttata tgtgattcaa gatattcagt aaccctgtct ataattactt
     5221 tactttgacg ttcaccgtct tgatacaacg cttgtgctaa tagcaaactt ttacttaaat
     5281 gtaccgcttc ttgtcgttct tgctccgtta aataaacatt tcttgaactt ttcgccaaac
     5341 catctgcttc tcgaacgata tcaataccaa taatttcaac ggcatgattg aagtctttta
     5401 ccatttgctc gacaatagcc aattgctggg catctttttt accaaaataa gcataatccg
     5461 gcataacaat attaaatagc ttattaacta ctgttaccac cccatcaaaa tgccctggac
     5521 gcttcgctcc ttctaacaca tcagctaatg ggcctacttt gacatcaata cctaattcac
     5581 ctggatacat atcttctact gcaggatgaa aaacaatgtc cgctcctact tctgatacta
     5641 attctaaatc tttatcaatt tgtctcggat aagcatcgaa atcttcgttt ggaccaaatt
     5701 gtaatggatt aacaaataca ctcacaattg taatatcatt tgtactaact gattcgcgta
     5761 ccatcgttaa atgtccatca tgtaaggcac ccattgttgg gataaaacca atcgttgtgc
     5821 ctgagcgttt ggctgcttta acaatgtgtt gcatctcttt taccgtagta atcagcttag
     5881 tcattgttat taacctcatt cataattttc tttttatacg tatattcttc tgatggaaat
     5941 gcaccagatt taacttcttg atcgtattgt tttaaaccat ccacaccaac actaaaatca
     6001 gcaaattgct tcacaaattt cgctttatgt tcaacaccat aatttaacat atcgtgataa
     6061 accaatactt gaccatctgt accttttcct gcaccaatac caatgactgg aattgttaag
     6121 tgcttgctaa tttcttctgc taaatcattt ggaattgctt caagtactaa cgcaactgca
     6181 ccagcttgtt ctacattttt cgcgtctaaa ataagttgct ccgctgcttc tttcgttgca
     6241 ccttgtaatt tataccccat aacgccaaca ctttgaggtg ttaatcctaa atgtgcaaca
     6301 acaggaatac caattgccgt tgctttttca ataaatggtg taatatgcgc tccttctgct
     6361 ttaattgcat ttgcattcgt ctcctgataa agctttagag catgatttaa gtcttgtgtc
     6421 atagagatgc ctactgcacc aatcggcata tcaacaacta caaatgtatt tggtgcgcct
     6481 cttcttactg cacgaccgtg atgaatcata tctgctaacg tcacttgtac ggtactttca
     6541 taacctaata cagtcatacc aagtgaatcc ccaacaagaa tcatatcaat acc
//
LOCUS       DALAGD010000124         6514 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.127, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000124 DALAGD010000000
VERSION     DALAGD010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6514
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.127"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(<1..803)
                     /locus_tag="O2701_002468"
     CDS             complement(<1..803)
                     /locus_tag="O2701_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCZ4294337.1"
                     /translation="MKRASYSVETKYKAVEMKAAGFPTKEIMKELNIRNRTQVKNWWR
                     WYRNGESYRFSQHVGKQYTYGYRKITALINQCYTSPINHKRVQRVMQKHHLNCRVRPK
                     KTTRIGKPYYKTDNLLQRQFKASCPMEVLTTDITYLPFGHSMLYLSSIMDIYNGEIVA
                     YKIDDKQDQRLVNDTLNQIDIPEGCILHSDQGSVYTSYAYYQLCEEKGIIRSMSRKGT
                     PADNAPIESFHSSLKSETFYINNELNHSNHIVIDIVEKYIKNNNNNNNNR"
     gene            complement(1315..1812)
                     /locus_tag="O2701_002469"
     CDS             complement(1315..1812)
                     /locus_tag="O2701_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012656763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4294338.1"
                     /translation="MKLRALEYSDLLFVHELNNEYSIMSYWFEEPYESLTELQHLFDK
                     HLLDESERRFIVEDENQVVGIVELVEINYIHRNCEIQIIIKPEFSGKGYAKFAFEKAI
                     IYAFNILNMHKIYLYVDADNKKAIHIYESQGFKTEGLLKEQFYTKGKYKDAYFMSLLK
                     SEYIL"
     gene            2310..3437
                     /locus_tag="O2701_002470"
     CDS             2310..3437
                     /locus_tag="O2701_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HCZ4294339.1"
                     /translation="MKTRAAVLHEMGAKKPYKESKPLKIEYLELDNPSEHEVLIKIHA
                     AGLCHSDLSVINGNRPRPLPMALGHEASGEVIKVGKAVTRVSEGDHVVCTFIPSCGKC
                     IPCKEGRPALCENGAISNEKGEMLEGGMRLSNDEGKVYHHLGISGFAEYSVVSENSIV
                     KIDKKIPFERAAAFGCAIITGIGAVVNTAQIRSGSNVAVVGLGGIGLNAIIGAKLAGA
                     NEIIALDINEDKFELAKQFGATATFNSSDKDIDEQIKEYIPGGVEYAFETAGVVPAMK
                     VAYQITKRGGTTVTTGLPNPKDNFSFPQVTLAAEERTIKGSYVGSCVPDRDIPRFVNL
                     YNQGRLNIDSLISEVITLDEINEGFDRLSNGEVGRIIMKMH"
     gene            complement(3692..3916)
                     /locus_tag="O2701_002471"
     CDS             complement(3692..3916)
                     /locus_tag="O2701_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294340.1"
                     /translation="MNHDNTIQSNWRTVASCLASQNYISIVKGLVHHFTAIDDEEILD
                     KIYEEFINDDSITTVLNNDLQTIINHYLSK"
     gene            complement(3930..4433)
                     /locus_tag="O2701_002472"
     CDS             complement(3930..4433)
                     /locus_tag="O2701_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012086592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HCZ4294341.1"
                     /translation="MNTIKSTIHTEAIFSDDEQHRYLLKKIWDDKKPACTVITMYLHL
                     DGVLSLDLTTVLILNQLANSEQYGAVYLVNLFSNIKTPENLKHIKEPYDEHTDIHLMK
                     AISESDTVILAYGAYAKRPVVIDRVEQVMEMLKPHKKKVKKLINPVTNEVMHPLNPKA
                     RQKWTLK"
     gene            complement(4449..4760)
                     /locus_tag="O2701_002473"
     CDS             complement(4449..4760)
                     /locus_tag="O2701_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294342.1"
                     /translation="MKINRYITRGISEQLSLDLQILLWNMVKDRDNQLNTDYLHIFKL
                     QEDDNMLSITHEQEQPAYKLEYHYTNYIKNQNALPKKVYVIREDDVDAFYYVMLLPEE
                     Y"
     gene            complement(4847..5197)
                     /locus_tag="O2701_002474"
     CDS             complement(4847..5197)
                     /locus_tag="O2701_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002502216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294343.1"
                     /translation="MKTTTQELKQYMTRLFQLSNNETWECETLEEAAENILPKRFIND
                     SPLAHLILETYTYYNNELHELSIYPFLMYSNNQLISIGYLDHFDMDFLYLTDTKNTII
                     DERHLLKEGGNNHE"
     gene            complement(5804..6018)
                     /locus_tag="O2701_002475"
                     /pseudo
     CDS             complement(5804..6018)
                     /locus_tag="O2701_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736766.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
     gene            6005..6514
                     /locus_tag="O2701_002476"
                     /pseudo
     CDS             6005..6514
                     /locus_tag="O2701_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558285.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2166 a          974 c         1052 g         2322 t
ORIGIN      
        1 cgattattat tattattatt attgttttta atgtactttt cgacaatatc tattacaatg
       61 tgattagagt gattaagctc attattgatg taaaaagttt cagactttag cgaggaatgg
      121 aaactttcta tcggggcgtt atcggcaggt gttccctttc gggacatact tctgataatg
      181 cctttttctt cgcataattg ataataagca taagatgtat aaacgctgcc ttgatcacta
      241 tgtaatatac acccctcagg tatatcgatt tgatttaatg tatcattaac taaacgttgg
      301 tcttgtttat catctatttt atacgccaca atttctccgt tataaatgtc cattatcgaa
      361 gataaataca acatagaatg accaaatggt aaataagtaa tatcggttgt taatacttcc
      421 atgggacaac tcgctttaaa ttgtctttgt aataaattgt ccgttttata atacggttta
      481 cctattcttg tcgtcttttt aggtctaact cggcagttca aatgatgctt ctgcatcact
      541 ctctgtactc tcttatgatt aattggtgat gtataacatt gattaatcaa tgctgtaatc
      601 ttacgataac cgtaggtata ttgtttacca acatgttgtg aaaatctata actttcccca
      661 tttcgatacc atcgccacca atttttcact tgtgttctat ttctaatatt taattctttc
      721 ataatttctt ttgttggaaa tcctgctgct ttcatttcaa ctgctttata ctttgtttct
      781 actgaataag aagctctttt catagaaaaa aacacctccg tatgattcat tttaatatga
      841 attcaacgaa agtgttttta tataattccc actattgggg tcagtctaaa attaaaattg
      901 ataacttttt taataaaata gatacactaa ttttaaaaca aggtaaaaaa atagaattat
      961 taaaacaacg caaacaaggc ttactacaaa aaatgtttgt gtaattctga taacgtccta
     1021 ttatgttaat tgaaaataga caaaaataaa ttgagagcat cccttaccgc aaagtgaagg
     1081 atgctctatt tttgttgaaa ttaatataag cttcattctg atagatagtc attaataatt
     1141 gtttgcaaat cgttattaag taccgttgtt acagcgtcat cattcataaa atcctcgtag
     1201 attttatcaa gaatttattc ctcttacaga cattcgcgag aagtccgttt taattttatt
     1261 agaagtaatt caggtttgaa cctacctaaa tgaatatatg agttatcttt tatactacaa
     1321 aatatattca gatttcaata atgacataaa ataggcatct ttatatttac ctttagtgta
     1381 gaattgctct ttaagtaatc cttctgtttt aaatccttgt gactcgtata tatgaatagc
     1441 ttttttgtta tctgcatcaa catatagata gatcttgtgc atatttaaaa tattgaatgc
     1501 ataaattatc gctttttcga atgcgaattt tgcataacct ttaccactga actcaggttt
     1561 aataattatt tgtatttcac aattacgatg gatgtaatta atttctacta attcaacaat
     1621 acctacgact tgattttcat cttcaacaat aaaacgtctt tccgattcat ctaataaatg
     1681 cttatcaaat aaatgttgaa gttccgttaa ggattcataa ggctcttcaa accaataaga
     1741 cataatagaa tattcattat ttaattcatg aacaaaaagt aaatcactat actctaatgc
     1801 tcttagtttc ataatcccac tcccaaaatt ttcttatata tttgcattat aaatataaat
     1861 aacgaataag tcattattca ctatgaataa atctatttta acaattcact acatactaat
     1921 tctcattttc ttattattct ctaatatctc tgatttatta ctcagtgaaa gatgccctat
     1981 ttttatcagt atatagtttt tattttgata gatattgatt aataatcgtc tgtaaatcat
     2041 tgttaagtaa ggttgtgata gcgtcgttat tcataaaatc ttcataaatt ttatcaagta
     2101 tttctttaat atatttttta atcttcacac cttaaattca cttgaaaacc aaatcacttc
     2161 taaattatga ttttacataa taaattttta aaaattggta ttatataaaa taatctcaat
     2221 aatcagaatt ttttgtaaat aataatatta aagtgtaaaa taacagataa gaatagactt
     2281 aatgtatata aattaaagga gtgttggaag tgaaaactag agcagcagtt ttacatgaaa
     2341 tgggggctaa aaagccctat aaagaatcta aacctttaaa aattgaatac ttagaacttg
     2401 ataatcctag cgaacacgaa gtattaatta aaattcatgc agctggatta tgtcactctg
     2461 atttgtcagt aattaatggt aacagaccta gacctttacc tatggcactt ggtcatgaag
     2521 cttccggtga agtaattaaa gttggaaaag ctgttacaag agttagcgaa ggagatcatg
     2581 tggtatgcac atttattccc agttgtggaa aatgtatccc atgtaaagaa ggacgtcctg
     2641 cattatgtga aaacggagca atatctaatg aaaaaggcga aatgttagag ggagggatgc
     2701 gtttatctaa tgatgaagga aaagtatatc atcacttggg gatatcaggt tttgctgaat
     2761 attctgttgt ctctgaaaac tctatagtta aaattgataa aaaaatacct tttgaacgtg
     2821 cagctgcatt tggttgtgct attatcacag gtatcggtgc tgtggtgaat acagcccaaa
     2881 ttcgttctgg tagtaatgta gcggttgttg gtttaggagg tattggattg aatgctatta
     2941 ttggagctaa actagcggga gccaatgaaa ttattgcttt agatattaac gaagataaat
     3001 ttgaattagc aaagcaattt ggggctacag caacatttaa ttcaagcgat aaagatatcg
     3061 atgagcaaat taaagaatat attcctggcg gagtagaata tgcttttgaa acagcgggtg
     3121 tagtgccagc tatgaaagtt gcttatcaga ttactaaacg aggggggaca actgtaacaa
     3181 caggactgcc taatcctaaa gataattttt ctttccctca agttacttta gcggctgaag
     3241 aacgtaccat taaaggatca tatgtaggaa gttgtgtacc tgacagagat ataccaaggt
     3301 tcgttaatct atacaaccaa ggacgtttaa atattgattc actcatcagt gaggttatca
     3361 ctttagatga aattaatgag ggatttgatc gtttgtctaa tggtgaagta ggtagaatta
     3421 taatgaagat gcattgaata taatagaatt caagtcgttc ctcctcttga tttctatgaa
     3481 aagaataaca atttgagtag gaagattact gacaaatatt acatttgcat tgtgactgcc
     3541 aatctaattg tatttttaca tttcatatct atatattaga agataaatgt tttaaaacta
     3601 attactaata gtttttttag ataaaaaaag atataggagc atccttcacc gcaaaagtga
     3661 aggatgctct agttttatta gaatatagtt ttcattttga tagataatga ttaataattg
     3721 tttgtaaatc attgttaagt accgttgtaa tagagtcatc atttataaac tcttcataga
     3781 ttttatcaag tatttcctcg tcatcgatgg ctgtgaaatg atgcaccaga ccttttacta
     3841 ttgaaatgta attttgtgat gctaaacaac tagcaaccgt tcgccaattg gattgtatag
     3901 tgttgtcatg gttcatagat aatactcctt tactttagtg tccatttttg acgtgcttta
     3961 gggttgagtg gatgcataac ttcgttcgtt actggattta tgagcttttt tactttcttt
     4021 ttatgaggtt ttaacatttc catcacttgt tcgacacggt cgataacaac tggtcgcttc
     4081 gcataggcac cataagcaag aatgactgtg tcactttcac taattgcttt cattaagtgt
     4141 atatctgtgt gctcatcata aggctctttg atatgtttaa ggttctctgg tgttttaata
     4201 ttagagaaaa gattaacgag ataaacagcg ccatattgct cagaattagc taattgattg
     4261 aggatgagaa ccgtagtaag atcgagtgat aatacaccat ctaaatgaag atacatcgtt
     4321 atcacagtac aagccggttt cttgtcatcc catatctttt tgagtaagta gcgatgttgt
     4381 tcatcatcgc taaatatcgc ttctgtgtgt atcgtacttt tgattgtatt catatatcgt
     4441 cacttccttt agtattcttc tggcaaaagc atcacataat aaaaagcgtc tacatcatct
     4501 tctcggatga cgtagacttt cttaggtaat gcattttgat tttttatata gtttgtatag
     4561 tgatattcca atttgtatgc gggttgttct tgttcatgtg taattgacaa catattatca
     4621 tcttcttgta gtttgaaaat gtgtaggtaa tctgtattaa gttgattgtc tcgatctttt
     4681 accatgttcc aaagtaagat ttgaaggtct agagatagtt gttcactaat gcctcttgtg
     4741 atgtatcgat tgattttcat actattttcc tccattttgc ttttctttca tgatgtcaat
     4801 cacttcgtta atgactgtaa cagatatttg tgccactttg atccaattat tcatggttat
     4861 tccctccttc ttttagtaaa tgacgttcat cgataatcgt atttttagta tctgtgaggt
     4921 atagaaagtc catatcaaaa tgatccagat aaccaatact gatgagttgg ttattagaat
     4981 acattagaaa tggatagata cttagttcat gtagctcatt attatagtag gtataagttt
     5041 caagtataag atgtgcaagt ggggaatcat taataaaacg tttgggtaga atattttctg
     5101 ccgcttcctc cagtgtttca cattcccatg tttcattgtt agataattgg aataagcgag
     5161 tcatatattg tttgagttct tgagtagttg ttttcatatc attgcctccc tagatagtgt
     5221 gatagtgatg tacttcatat acatcattga gataatatat atttgatttg tcatttatta
     5281 ctgatcccgt tgacaatatg agaaaattcc atataaaaaa ccgctacaaa ccttggtatg
     5341 acaaggaaat cccgaaattc cgcctatttt gacgaacaat caactcatta tttataagta
     5401 ttgatgatag ggtggggtct ctgcttcctt atatatatta tttatttata aagaataacg
     5461 ggattttggg attgtgcttg cacaatcctt ctgcttcttc taatctgcaa atcccattcc
     5521 tttcccgata aaaaatcatc gtgggatgtt ctttagcaat ttcaatataa gcctcgtgta
     5581 gttatgaaaa aattacgaca atgactgttt cattagataa gtgttattga aattgataaa
     5641 gagaattctc aaaaattttt agaaaaagaa agaggaagta tgtagatata aaatagattt
     5701 attaaatagt aaggtataat gtttgcggta aaaataaaga cgaagtgctg gaatacactt
     5761 cgtcgataat aaggtagttg aagtttcgat tattttaaat tgatcatttt aagcctacaa
     5821 atccctctaa tgtttcacca gatttgtaca cttttgtcgc cttctttttc ctaaataacg
     5881 taaatgccaa ggttcatatt gatatcctgt gatgctttct ttgtttttag gatatcttat
     5941 aataaatcca taattatggg cattcttagc tatccagcgt ccttcctcag ttttaccaaa
     6001 actagcatat agattttcac aacaagttgg taaacaatat acctacggta aaggattaga
     6061 agagctgtca gaagtagaac aattaaaatt agaaaataag agaaaagata tagaattgga
     6121 tattttaaaa aagtacaagg tattggaaag gaagtggtac caacagtagt catagattta
     6181 gtggatcaat taaaagtaaa atattcaatc aaattgctac taaaagtatt aaacatacct
     6241 aaatcaacat attaccgatg gaaaaacaaa acccataaaa atgataccgt aacacaaaaa
     6301 gttattgaat tatgtaaagc taaccactat acctacggtt atcgtaagat tacagcattg
     6361 attaatcaat gttatacatc accaattaat cataagagag tacagagaat gatgcagaag
     6421 catcatttga actgccgagt tagacctaaa aagatgacaa aaataggtaa accgtattat
     6481 aaaacggaca atttattaca aagacaattt aaag
//
LOCUS       DALAGD010000125         6495 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.128, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000125 DALAGD010000000
VERSION     DALAGD010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6495
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.128"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(169..1242)
                     /locus_tag="O2701_002477"
     CDS             complement(169..1242)
                     /locus_tag="O2701_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017328539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HCZ4294344.1"
                     /translation="MNIVKLMVIIIYLIIGSALGIIIIPEIANDLGLQNSSFLKNHYV
                     DGIIGSIFMFLIFGVFIRRVTNAIKGLEHFIMRRSAVEILFATIGLIIGLLISVMVSF
                     ILESIGNSIFNHFIPVIITILLCYFGFQFGLKKRDEMLMFLPENIARSMSQHTKSATP
                     KIIDTSAIIDGRILEVIRCGFIDGNILIPQGVINELQIVADSNDSVKREKGKRGLDIL
                     NELYDLDYPTKVIHPTKTHSDIDTMLLKLAKQYHASIITTDFNLNKVCHVHGIKALNV
                     NDLSEAIKPNVHQGDQLHILLTKMGKEPGQAVGYLDDGTMVVVDNAKNLIGSHVNLEV
                     VSLLQTSSGRIVFAKKIEDTVSL"
     gene            complement(1267..2631)
                     /gene="radA"
                     /locus_tag="O2701_002478"
     CDS             complement(1267..2631)
                     /gene="radA"
                     /locus_tag="O2701_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCZ4294345.1"
                     /translation="MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPK
                     HGVKTKELAGKVQKLNSIKHETTPRVLTDSAEFNRVLGGGIVSGSLVLIGGDPGIGKS
                     TLLLQICASLSQKKKVLYITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVK
                     EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEG
                     QIAGPRLLEHMVDTVLYFEGDEHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNP
                     SEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPRRMATGIDHNRLSLL
                     MAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGE
                     VGLTGEVRRVSRIEQRVQEAAKLGFKRVIIPKNNIGGWTYPEGIQVIGVTTVHEVLSF
                     ALHS"
     gene            complement(3116..5572)
                     /locus_tag="O2701_002479"
     CDS             complement(3116..5572)
                     /locus_tag="O2701_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCZ4294346.1"
                     /translation="MLFGRLTERAQRVLAHAQEEAIRLNHSNIGTEHLLLGLMKEPEG
                     IAAKVLESFNITEDKVIEEVEKLIGHGQDHVGTLHYTPRAKKVIELSMDEARKLHHNF
                     VGTEHILLGLIRENEGVAARVFANLDLNITKARAQVVKALGNPEMSNKNAQASKSNNT
                     PTLDSLARDLTVIAKDGTLDPVIGRDKEITRVIEVLSRRTKNNPVLIGEPGVGKTAIA
                     EGLAQAIVNNEVPETLKDKRVMSLDMGTVVAGTKYRGEFEERLKKVMEEIQQAGNVIL
                     FIDELHTLVGAGGAEGAIDASNILKPALARGELQCIGATTLDEYRKNIEKDAALERRF
                     QPVQVDEPSVVDTVAILKGLRDRYEAHHRINISDEAIEAAVKLSNRYVSDRFLPDKAI
                     DLIDEASSKVRLKSHTTPNNLKEIEQEIEKVKNEKDAAVHAQEFENAANLRDKQTKLE
                     KQYEEAKNEWKNAQNGMSTSLSEEDIAEVIAGWTGIPLTKINETESEKLLSLEDTLHE
                     RVIGQKDAVNSISKAVRRARAGLKDPKRPIGSFIFLGPTGVGKTELARALAESMFGDD
                     DAMIRVDMSEFMEKHAVSRLVGAPPGYVGHDDGGQLTEKVRRKPYSVILFDEIEKAHP
                     DVFNILLQVLDDGHLTDTKGRTVDFRNTIIIMTSNVGAQELQDQRFAGFGGSSDGQDY
                     ETIRKTMLKELKNSFRPEFLNRVDDIIVFHKLTKEELKEIVTMMVNKLTNRLSEQNIN
                     IIVTDKAKDKIAEEGYDPEYGARPLIRAIQKTIEDNLSELILDGNQIEGKKVTVDHDG
                     KEFKYDIAEQTSETKTPSQA"
     gene            complement(5586..>6495)
                     /locus_tag="O2701_002480"
     CDS             complement(5586..>6495)
                     /locus_tag="O2701_002480"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HCZ4294347.1"
                     /translation="ENHVHPLMYATENDGFRVINEVQDALPNFELMRLDQMDQQSKMK
                     MVAKHLISPELIKQPAAAVLVNDDESLSVMINEEDHIRIQAMGTDTTLQALYNQASSI
                     DDELDRSLDISYDEQLGYLTTCPTNIGTGMRASVMLHLPGLSIMKRMTRIAQTINRFG
                     YTIRGIYGEGSQVYGHTYQVSNQLTLGKSELEIIETLTEVVNQIIHEEKQIRQKLDTY
                     NQLETQDRVFRSLGILQNCRMITMEEASYRLSEVKLGIDLNYIELQNFKFNELMVAIQ
                     SPFLLDEEDDKSVKEKRADILREHIK"
BASE COUNT         1932 a         1264 c          882 g         2417 t
ORIGIN      
        1 tatctaaaat ttaacactta atgaacttgt ttcaatgata tattagcact atttgtattt
       61 tttgataact aatatgtttt gcatttattt atagttatac ttcaaattac aaacttcgcc
      121 atttcatata ccttttaata tctattttgt tttcgtcaac tacagttttt ataatgatac
      181 tgtatcttcg atttttttag caaaaacaat tcttcctgaa gatgtttgca ataagctgac
      241 tacttctaaa ttgacatgac tgccaataag atttttagca ttatcaacaa ctaccatcgt
      301 accatcatct agatatccta ctgcctgacc aggctcctta cccatttttg tcagtaaaat
      361 atgcagttga tcaccttgat gtacattagg tttgattgct tctgataaat cattaacatt
      421 taatgctttg ataccatgta catgacaaac tttatttagg ttgaaatctg tcgttataat
      481 acttgcatga tattgttttg ctaattttaa taacatcgta tcaatatcac tatgtgtttt
      541 agttggatgt ataacctttg taggatagtc taaatcatac aattcattta aaatatctaa
      601 gcctctttta cccttttcac gtttaacact gtcatttgaa tctgcaacaa tttgtaattc
      661 attaataaca ccttgtggaa ttaaaatatt gccatcgata aaaccgcaac gaatgacttc
      721 taaaatacga ccatcaataa ttgcgcttgt gtcgataatt tttggcgtag cacttttagt
      781 atgttgtgac atggaacgcg ctatattctc aggtaaaaac attaacattt catctcgttt
      841 tttaaggcca aattggaaac cgaaataaca tagtaatatc gtaattatga caggaatgaa
      901 atgattaaaa atagagttgc caattgattc taatataaac gacaccataa cagaaataag
      961 taatccgatt attaaaccta ttgttgcgaa tagtatttca acagcacttc tacgcataat
     1021 aaaatgttct aaacctttta tagcgttagt aactcgtcta ataaatacac caaaaattaa
     1081 gaacataaaa atactaccga taatgccatc tacatagtga ttttttaaaa agctggagtt
     1141 ttgtaatcca agatcatttg caatttcagg aataataatt attcctaatg cgctcccaat
     1201 aattaagtaa ataataataa ccattagttt aacgatattc acacaatgtc ctcctttctt
     1261 gatgttttat gaatgaagag caaatgacaa tacttcatgt acagtagtta cacctattac
     1321 ttgtatacct tcaggatatg tccatccgcc tatattattt ttaggaataa ttacacgttt
     1381 gaaacctagt tttgcagcct cttgcacgcg ttgttctatc cgagatacac gacgtacctc
     1441 acccgttaaa ccaacttctc caatatagca atctaatccg tcgacagctt tatctttaaa
     1501 gctagatgca gttgctacaa ttacacttaa atcaactgct ggctccgtta actttacacc
     1561 gccagctact ttgatataag catcttgttg ttgtaataga taattttctt tcttttccaa
     1621 aacagccatc aacaaactta atcgattatg atcaatccct gttgccattc gtctcggatt
     1681 gttaaaagtc gttggagtta ccagcgcttg aacttctatt aaaagtggtc tggttccctc
     1741 catggttgca acaattgttg aacctggaac atttgttgaa cgttcttcta aaaacatttc
     1801 agatggatta tttacacctt ttaatccact ttgcttcatt tcgaagattc ccatttcatt
     1861 cgttgaacca aaacggtttt taacagctcg caaaattcga tatgcgtggt gttcatcgcc
     1921 ttcaaaataa agcacagtat caaccatgtg ttctagcaat cttgggccag caatttgacc
     1981 ttctttcgtt acatgaccca ctataaaagt tgcaatgttc atttgtttag caatattcat
     2041 taaactttgt gtactttcac gaacttgtga aacagaacct ggcgcagagc tgatttcagg
     2101 atgatatatt gtttgaatcg aatccactac taataaatca ggttgttctt cttttactgt
     2161 ttgataaata acttcaagat ctgtttcagc taatacttgc aattcacttg aatcttcatc
     2221 taatcgctct gcacgtaatt tagtctgact aagcgattct tctccagtaa tatatagtac
     2281 ttttttcttt tgagataacg atgcacaaat ttgtaaaagt aacgttgact taccaatacc
     2341 tggatcccca ccaataagta ctaacgatcc gctcacaata cctccaccta atacacggtt
     2401 gaattctgct gaatctgtta acactctcgg cgttgtttca tgtttaatac tatttaattt
     2461 ttgtacttta cctgctaatt ccttggtttt aactccatgt ttaggattgg ctgctttttc
     2521 aacaatttcc tccatttgat tccaagcgcc acaattagga catttcccca tccatttagg
     2581 agattgataa ccacaagcca tacattcaaa aatcactttt ttcttggcca aaattgcacc
     2641 tccactttcc tttgaacaaa tctattttaa acgcacattc ccattatgac aaattaaatg
     2701 tgagttacat ttgtttgtat tttaacatga ctactaacgc aaactgtttt accagattta
     2761 ttttttatga atattataaa ataaacttac atcatgttga tagtcggtat ctttcaaaga
     2821 taaaatctta ataatttctt agtaaactct tttctctaga tttatcacaa tattatatag
     2881 acctatttta ttttgacgta agttgctagt atcttcaaac aaaaaacctt tataaaaatt
     2941 cataccttta tgctatcgct gtaggctcat taacttgtta catataattc ttaactatcc
     3001 tttgatgatt gttttattag attgtttcgt tgatggatac tttcacgaat ttctatagtt
     3061 caatgctact aaaaaaacag cccaaaactt taatttgttt tggactgttt tataattatg
     3121 cttgcgatgg tgttttagtt tctgaagttt gttcagcaat gtcatattta aactctttac
     3181 catcatgatc tactgtaact ttcttacctt caatttgatt accatctaat attaattcac
     3241 ttaaattatc ttcgatagtt ttttgtatcg ctctaattaa tggtcttgca ccatattctg
     3301 gatcatatcc ttcttctgcg attttgtctt tcgctttatc agttacaata atatttatgt
     3361 tttgttcaga taatcgattt gttaatttat taaccatcat tgttacaatt tcttttaatt
     3421 cttcttttgt tagtttatgg aatacaatga tatcatctac acggtttaaa aattctggac
     3481 ggaatgaatt ttttaattct tttaacatcg tttttcgaat tgtttcataa tcttgtccat
     3541 cacttgaacc accgaatcca gcaaatcgtt gatcttgtaa ttcttgtgcc ccaacgtttg
     3601 atgtcattat gataattgta tttctgaaat caactgtacg tccttttgta tctgtcaaat
     3661 gtccatcatc taaaacttgt aatagaatat taaatacatc tggatgagct ttttcaattt
     3721 catcaaataa aattacagaa tatggtttac gtctaacttt ttcagttaat tgtccaccat
     3781 catcatgacc aacatatcct ggaggagcac caactaatcg gctcactgcg tgtttttcca
     3841 taaattcact catgtctaca cggatcatcg catcatcatc gccaaacatt gattcagcta
     3901 aagctctagc taattcagtt ttaccaacac cagttggtcc aaggaagata aagctaccaa
     3961 ttggtcgttt aggatctttt aaccctgcac gggcacgtct aaccgcttta ctgattgaat
     4021 taacagcatc tttttgccca ataactctct catgtaatgt atcttctaga ctaagaagtt
     4081 tttcagattc tgtttcattg attttagtta atgggatacc tgtccatcct gcaataactt
     4141 cagcaatatc ttcttctgac aatgaagttg acatgccatt ttgtgcattc ttccattcat
     4201 ttttagcttc ttcatattgc ttttcaagtt ttgtttgttt atcacgcagg ttagcagcat
     4261 tttcaaactc ttgagcatgt actgcggcat ctttttcatt tttaactttt tcaatttctt
     4321 gttcaatttc ttttaaatta ttaggtgtcg tatgactctt aagtcttact ttagaacttg
     4381 cttcatcaat taaatcaatt gctttatctg gtaagaaacg atctgaaacg tatctgttac
     4441 ttaatttaac agctgcttca atagcttcgt ctgaaatatt aatacgatgg tgtgcttcgt
     4501 aacgatctct taatcctttt aaaatagcaa ctgtatctac tactgaaggt tcatcaactt
     4561 gtacaggttg gaaacgacgt tctaaagccg cgtctttttc aatatttttg cgatattcat
     4621 ctaatgtagt agcaccaata cattgtaatt caccacgtgc taatgccggc ttcaaaatat
     4681 tcgaagcatc gatagcacct tcagcaccac cagcaccaac taaagtatgc aactcatcaa
     4741 taaataggat gacattacct gcttgttgga tttcttccat aacctttttc agacgctctt
     4801 caaattcacc acgatattta gtacctgcaa ctactgttcc catatctaaa gacataacac
     4861 gcttatcttt taatgtctct ggtacctcat tattcactat ggcttgcgct aaaccttcag
     4921 caatagcagt tttaccaaca cctggctctc caataagcac aggattgttt ttcgtacgtc
     4981 tacttaatac ttcaattaca cgtgtaattt ctttatcacg tcctataaca ggatctaatg
     5041 taccgtcttt ggcaatgact gttaagtcac gagctaaact atctaaagtt ggagtattat
     5101 ttgacttact agcttgtgca tttttattac tcatttcagg gtttcctaaa gctttcacaa
     5161 cttgtgcacg tgctttagta atatttaaat ctagatttgc aaaaactctt gctgcaacac
     5221 cttcattttc acgaatcaag cctaataaaa tatgttccgt tccaacaaaa ttgtgatgta
     5281 attttctagc ttcatccatc gataattcaa tgactttttt agctctaggt gtataatgca
     5341 atgtaccaac atgatcttga ccatgtccga ttaatttttc aacttcttca attactttat
     5401 cttcagtgat attaaaactt tctaatactt ttgcagcaat tccttcaggt tctttcatta
     5461 accccaataa taggtgttct gttcctatat ttgaatgatt taaacgaatt gcttcttctt
     5521 gggcatgtgc taatacgcgc tgtgcacgct cagttaatct accaaataac ataaataatg
     5581 acctcctact ttatatgttc tcttagtata tctgctcgtt tttcttttac agatttgtca
     5641 tcttcttcat ctaataaaaa tggtgactgt atagctacca tcaattcatt aaatttaaag
     5701 ttttgtaatt caatgtaatt taaatctata ccaagtttaa cttcgcttaa tctataagaa
     5761 gcctcttcca tagttatcat tctacagttt tgtaaaatac ctagcgagcg aaaaacacgg
     5821 tcttgtgttt ctaattgatt ataagtgtct aacttttgtc gtatttgttt ttcttcatga
     5881 atgatttgat taacaacttc tgttaatgtt tctatgattt ctaactcaga tttaccaagt
     5941 gtaagttggt tggatacttg ataagtatgt ccataaactt gcgaaccttc accgtaaata
     6001 cctctgattg tatatccaaa acgattaatg gtttgagcaa tccgtgtcat tcttttcata
     6061 atagatagac ctggcaaatg tagcatcacg cttgctctca taccagtacc tatattggta
     6121 ggacatgtag ttaaataacc aagttgttca tcataactta tatcaaggct tcgatctaat
     6181 tcatcatcaa ttgatgaagc ttgattatat aaagcctgta atgtcgtgtc agttcccata
     6241 gcttgaatac gaatatggtc ctcttcattt atcatgacac ttaaagattc atcgtcattc
     6301 actaatactg cggctgctgg ttgttttatt agttcaggac taatcaaatg ctttgcaacc
     6361 attttcattt tactttgttg atccatttga tcaagacgca ttaattcaaa gtttggcaag
     6421 gcatcttgta cctcatttat aactctaaat ccatcatttt cagtagcata cattagtgga
     6481 tgcacatgat tttct
//
LOCUS       DALAGD010000126         6185 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.129, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000126 DALAGD010000000
VERSION     DALAGD010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6185
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.129"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(251..1129)
                     /locus_tag="O2701_002481"
     CDS             complement(251..1129)
                     /locus_tag="O2701_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HCZ4294348.1"
                     /translation="MPNKILLVDGMALLFRHFYATSLHKQFMYNSQGEPTNGIQGFVR
                     HIFSAIHEIRPTHVAVCWDMGQSTFRNDMFDGYKQNRSAPPEELIPQFDYVKEISEQF
                     GFVNIGVKNYEADDVIGTLAQQYSTDNDVYIITGDKDLLQCINDNVEVWLIKKGFNIY
                     NRYTLHRFNEEYALEPQQLIDIKAFMGDTADGYAGVKGIGEKTAIKLIQQYQSVENVV
                     ENIDALSAGQRNKINDNLDELYLSKRLAEIHTQVPIDSEALFEKMSFATTLNHILSIC
                     NEHELHVSGKYISSHF"
     gene            complement(1149..4589)
                     /locus_tag="O2701_002482"
     CDS             complement(1149..4589)
                     /locus_tag="O2701_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HCZ4294349.1"
                     /translation="MINKEQLDLLYKLKKEVEKSRNEALLHTINQVIKKVYLQQYTCS
                     FVGHFSAGKSTLINLLIEQDILPSSPVPTTSNTAIVSVSDNHDIIANLPNQTYAKLSN
                     YDEVREMNRQNVDVESVEINFQSAKFENGFTLQDTPGVDSNVASHQSITEQYMYTSNM
                     IFYTVDYNHVQSELNFKFMKHINDVGIPVVFIINQIDKHQDDELSFSTFKSRVEKSIA
                     DWGIKLERTFYVSKFDHPENELEVLSSYLISLDQHRETIEDYTSRTVEYITEAQLDYI
                     QSEIQEVLEDLGIEEAEFEQAFLNSQQHQAISEEAQLLNNPDELMAFLKNKRKNILEN
                     AYIMPHNMREMLRSYLESMSQDFNVGGFFNKKKKKLQIQQQRLLTATDALQEHVNQQI
                     RQPMREDMSFVTRFINKKEASDKVLNQHYDVKPEMIEGLYQPQTSISNTYVLTFSDEV
                     VKAIKKYVEQQSTPIFKEIIENVQADELPTEESDDLKEYQRYTELNELRQSLTTKNYR
                     HYYIHLDESLDKLIGRQETTYQVATDNAQDNRDNQQLNQNTATTNMSIDIQKALDIIS
                     DVPLFKRTKQDIHETLTRIDNKLIKIGVFGTFSAGKSSLINALLGEHILVSSPNPTTA
                     ATTEISYGDESYITLKSQSQLLDEINAVVEYQDMSYSTIEDFINSDLEKLKSHLNKNQ
                     LAFIQAVEKHYKLYVNMLENGEKHAINQQELKKWSAEDEYATFVKTVHIALMHDWLKG
                     KIIVDSLGLHSNNQRHTNETEQILTSSDLILYVSYFNHSFTDNDKAFIEHMKDMNQLN
                     ENQALKMVINAADLAESQDDLEAVETYVSDALRQVHLQSDIFAVSSRNALQAEDKGID
                     QLKQSIQQFVDVESKSILEQQMIHQLQQMDRSYVEMITEFETNKADISRRQQRLTDYK
                     DKQRLQHQLIDATLQHTDNEVEEQVYHLNARLKLQLLDDVKSVFNSQMTQNSDFNEEK
                     KVSTRVYLDQIHQRLFLEQSLITERIKKYFNKQLTEQIAPIVQQLADLHVIINPQFNF
                     ESANIEQPLLHIDFNDMLNALPKQLTKRKILNPNGQRDIHESICQSTLELLQPQMGLL
                     RQQLELYVKQMAVEAESQFESFEANIQTQINDLLAFDLDTTLINQLKDKHQQLKTILY
                     "
     gene            4893..>6185
                     /locus_tag="O2701_002483"
     CDS             4893..>6185
                     /locus_tag="O2701_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCZ4294350.1"
                     /translation="MKNTFNPLQRLHFYAAIFIAPLLITLTISGIGYLFFPEVENNIY
                     KNEFFGDSDVKTHQTLNDAVHQVEQQYEGFFVSKVSILDEPYNKRITLSDMAGNQRYV
                     FLDHNNQFVADQNAKHTYSNVMRSIHSSLFTENTIINYLVELTACWMIFMILSGTYLL
                     IKKHLISNKSKALRWQKWHAMIGVIIAIPVFVLVLTGLPWSGFMGSKIAGMMDTNGAL
                     GQGELAINPPKSDLNELPWATRKNKQPASSEKGSSGHHGNAAMPQTKLDYQISIDKVV
                     EQAQKAGIKKPFSIVYPSDKNGTFIVSNTSNSGVTGLDVSPYKEQTLYFDQYSGKKLG
                     TIKYDDYGIIAKWFTWGIPLHEGHLFGILNKIINLFVCIALLVAIGMGFVSWIKRTKN
                     TAIKVPHRVKKPASISLIICLIVLGLLMPLFGLSLILVF"
BASE COUNT         1929 a          998 c          919 g         2339 t
ORIGIN      
        1 taattaatca aatatgtttt ttagattaat aaagttgtaa ctgtactatt ttgtagtgta
       61 tggtaattta tttggatgaa atataattct ttatactcta ttgattaatc aaatatgtat
      121 ctatcaaaat tcggcttatt tattcactct gacatatcca aatacgcaaa aagactattt
      181 ctaccttgta tcgatagaaa tagtcttttt atacttaatt ggtttaattt ttcataaaat
      241 cttatacttt ttaaaaatgg gatgaaatat attttcctga aacatgtaat tcatgttcgt
      301 tacaaattga taaaatatga ttaagcgttg ttgcaaatga cattttctca aaaagcgctt
      361 cactatcaat tggtacttgt gtgtgaattt cagccagtcg cttcgataaa tatagttcat
      421 ctaaattgtc attaatctta ttacgttgcc cagctgataa tgcatcaatg ttttcaacca
      481 cattttcaac gctttgatat tgctgaatta acttaatagc cgttttctcg ccaatacctt
      541 taacacccgc atatccatct gctgtatcac ccataaacgc tttaatatcg attagttgtt
      601 gtggttcaag ggcatattct tcgttaaaac gatgtaatgt atatctatta taaatgttaa
      661 aacctttttt aattagccaa acttcaacat tgtcattaat acattgcagt aaatctttgt
      721 cgcccgtaat aatatagaca tcgttatcag ttgaatattg ttgtgctaat gtacctataa
      781 catcatccgc ttcatagttt ttaacgccaa tatttacaaa gccaaattgc tctgaaattt
      841 ctttaacata atcaaattgt ggtatcaatt cttctggtgg tgcagaacga ttttgcttat
      901 aaccatcaaa catatcattt ctaaaagttg attgtcccat atcccaacat acagctacat
      961 gtgtagggcg tatttcatgt attgccgaaa agatatgacg cacaaatcct tgtattccat
     1021 ttgtaggttc accttgtgaa ttgtacataa attgtttatg aagacttgta gcatagaaat
     1081 gtctaaataa tagcgccata ccatctacaa gtaatatttt attaggcatt taaaacgttc
     1141 cttctttctt aatataaaat agttttcagt tgttgatgtt tatctttcaa ttgattgata
     1201 agtgttgtat ctaaatcaaa tgctaataaa tcgtttattt gcgtttgaat attagcttca
     1261 aaactttcaa attgcgattc agcttctaca gccatttgct ttacatataa ttcaagctgt
     1321 tgcctcaata atcccatttg tggttgtaat aattctaacg tactttgaca aattgattca
     1381 tgtatatctc tttgcccatt tggattcaaa attttacgtt ttgttaattg tttaggcaat
     1441 gcatttagca tatcgttgaa atcgatgtgc aataatggtt gctctatatt agctgattca
     1501 aagttaaact gaggattaat aatgacatgt aaatctgcta attgttgaac gattggtgca
     1561 atttgctcag tgagttgctt attaaagtat ttttttatac gttctgtaat taaagattgc
     1621 tctaaaaaca atcgttgatg aatttgatct aagtatactc tcgtagacac tttcttttct
     1681 tcattaaaat cactattttg cgtcatttga gaattaaaca ctgatttaac atcgtcaagt
     1741 agttgtagtt ttaaacgggc atttaaatga taaacttgtt cttcaacttc gttgtctgta
     1801 tgttgtaacg tcgcatcaat taattgatgt tgtaaacgct gtttatcttt ataatctgtt
     1861 aacctttgtt ggcgacgtga aatatcagct ttatttgttt caaattctgt aatcatctct
     1921 acataagaac gatccatttg ttgaagctga tgaatcattt gttgttctaa aattgattta
     1981 gattcaacat caacaaattg ttgtatgctt tgttttaatt gatcaatgcc cttatcttca
     2041 gcttgcaatg catttcgact tgatacagca aaaatgtctg attgtaagtg tacttgtctt
     2101 aatgcatctg atacatatgt ttcaactgct tcaagatcat cttgactttc tgctaaatca
     2161 gcagcattaa ttaccatttt taatgcttgg ttttcattca actggttcat atctttcatg
     2221 tgttctataa acgctttgtc attatcagta aatgaatgat taaaataact tacatacaat
     2281 attaagtctg aagaagttaa aatttgttcg gtttcatttg tatgcctttg gttatttgag
     2341 tgtagcccta atgaatcaac aattatttta ccttttaacc aatcatgcat taatgcaatg
     2401 tgtactgtct taacaaatgt tgcatattca tcttctgcac tccacttttt caattcctgt
     2461 tgattaatgg catgtttttc tccattttcc aacatattga catacaattt ataatgtttt
     2521 tcaactgcct gaataaaagc gagttgattt ttatttaaat gtgattttaa cttttctaaa
     2581 tctgaattaa taaagtcttc tatagtcgaa taagacatat cttggtattc aactactgca
     2641 ttaatttcat ctaataattg cgattgtgat tttagcgtta tataactctc gtctccataa
     2701 gatatttctg tagtagctgc tgtagtagga tttggagaac tgactaaaat atgctcgcct
     2761 aataatgcat ttatcaaact acttttacca gcactaaatg ttccaaatac acctattttt
     2821 attaatttat tatctatacg tgttaatgtt tcgtggatat cttgctttgt acgcttgaac
     2881 aaaggcacat ccgaaattat atcaagcgct ttttgaatat ctatagacat atttgttgta
     2941 gctgtatttt gatttagctg ctgattatca cgattatcct gagcattatc agtagccact
     3001 tgatatgttg tctcttgtcg acctattaat ttatctagag attcatctaa atgaatatag
     3061 tagtgacgat aattcttagt cgtcaatgac tgacgcagct catttaattc tgtataacgt
     3121 tgatattctt ttaaatcatc actttcttct gttggtaatt catctgcctg cacattttct
     3181 attatttctt taaaaattgg tgttgattgt tgttcaacat atttcttaat ggctttaacc
     3241 acttcgtctg aaaatgtaag tacataagta ttgctgattg atgtttgtgg ttgatataaa
     3301 ccttcaatca tttctggctt aacgtcataa tgctgattta atactttatc tgaagcttct
     3361 tttttattga taaaacgcgt aacaaatgac atatcttctc gcattggttg acgaatttgt
     3421 tgattaacat gttcttgtaa cgcatctgtc gctgttaata atcgctgttg ttgaatttgt
     3481 agttttttct tctttttatt aaaaaatccg ccaacattaa agtcttgaga catactttcc
     3541 aaataacttc gtaacatttc tctcatatta tgcggcataa tgtatgcatt ttctaaaata
     3601 tttttacgct tattctttaa aaatgccatt aattcatctg gattatttaa aagttgtgcc
     3661 tcttcactaa ttgcttgatg ttgttgacta tttaaaaatg cttgttcaaa ctccgcttct
     3721 tcgataccta aatcttctag tacttcctga atttcagact gaatgtaatc tagctgagct
     3781 tcggtaatgt attcaaccgt tcttgatgta taatcctcta ttgtctctct atgttgatct
     3841 aatgaaatta gataacttga taatacttca agttcatttt cagggtgatc aaatttagat
     3901 acataaaagg tgcgttctaa tttaataccc caatctgcaa ttgatttttc aactcgagat
     3961 ttaaacgtag agaatgacaa ttcatcgtct tgatgcttgt caatttgatt aatgataaac
     4021 acaacaggta ttccaacatc atttatatgc ttcataaact taaagttaag ttcagattga
     4081 acgtggttat agtcaaccgt ataaaatatc atattacttg tatacatata ttgttctgtt
     4141 attgactgat gtgatgcaac atttgaatca acacctggtg tatcttgcaa cgtaaaccca
     4201 ttttcaaatt tagctgattg aaaattaatt tctacagatt caacgtcgac attttggcga
     4261 ttcatttccc ttacttcatc ataattagat aatttggcat acgtttgatt cggcaaatta
     4321 gcaataatat cgtgattgtc tgaaactgac acaatagcag tattacttgt cgttggtaca
     4381 ggtgaacttg gtaagatatc ttgttcaatt aataaattta tcagtgtcga tttacctgca
     4441 gaaaaatgtc caacgaacga acatgtatat tgctgcaaat atactttctt aattacttgg
     4501 ttaattgtat gtaaaagtgc ttcatttcgc gacttttcaa cttctttttt taatttatat
     4561 aaaagatcta attgttcttt attaatcatg ttactttccc ctttggttcg ttcacataga
     4621 aatgcttgct tgttacaacg aattcataca atatcgatgt ccatttaaac atagatcgtt
     4681 aaatggctcg cattgtatta tgtttaaatt ttatcatagc tgcttgataa aacatacgaa
     4741 taatcaattt tcttcaaaca ttccaactta aaagttcatt tttctaaaaa tcacaaatca
     4801 tcgttaaatt tttctagaaa ataaatattg tcacagtttg ttcacacaca tcacagaatt
     4861 gacaccgtat aatagccaat tgggagggaa atatgaaaaa tacatttaat ccattacaaa
     4921 gattacattt ctatgcagca atatttattg ctccactgtt aatcacttta accatttcgg
     4981 gcattggtta cttgttcttt ccagaagttg aaaataatat ttataagaat gagttttttg
     5041 gtgacagtga tgtaaaaacg catcaaacat taaatgatgc agtacatcaa gttgaacaac
     5101 aatatgaagg attctttgta agtaaagtta gcatacttga tgaaccatat aacaaacgaa
     5161 ttacactgag tgatatggca ggaaatcaac gttacgtctt tctagatcat aacaatcaat
     5221 ttgttgcaga tcaaaatgcg aaacatacgt attctaatgt gatgcgaagt atacatagtt
     5281 ctttgtttac tgaaaatact attattaatt atttagtaga gttaaccgca tgttggatga
     5341 tattcatgat tttatctggt acttatttac tcattaagaa gcatttaatt tctaacaaaa
     5401 gtaaggcact tcgttggcaa aagtggcacg caatgattgg agttatcatt gcaattccag
     5461 tatttgtatt agtcttaact ggattgccat ggtctggttt tatgggcagt aaaattgccg
     5521 gtatgatgga cacaaacggt gcccttggtc aaggtgaatt agcgattaat ccacctaaat
     5581 cagatttgaa cgaattacct tgggctacac gtaaaaataa acagccagct tcatccgaaa
     5641 aaggttcaag tggtcatcat ggtaatgcag caatgcctca aaccaaatta gattatcaaa
     5701 tatctattga taaggtcgtt gaacaggcgc aaaaagctgg tattaaaaag ccgttttcaa
     5761 tcgtatatcc aagtgataaa aatggtacct ttattgtatc taatactagt aattcaggtg
     5821 ttactgggct agatgtatca ccatacaagg aacaaacact ttatttcgat caatatagcg
     5881 gtaaaaagct aggtacgatt aaatatgatg actacggtat tattgctaaa tggtttacat
     5941 ggggcattcc gcttcacgaa ggtcatttat tcggcatttt aaataaaatc attaatttat
     6001 ttgtatgtat cgctttatta gtagccattg gcatggggtt tgtctcttgg ataaagcgta
     6061 caaaaaatac tgcaataaaa gtaccacatc gcgtaaaaaa accagcatct atatcactca
     6121 taatatgttt aattgtatta ggattattaa tgccattatt tggattatca cttatccttg
     6181 tattt
//
LOCUS       DALAGD010000127         5953 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.130, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000127 DALAGD010000000
VERSION     DALAGD010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5953
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.130"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            197..559
                     /locus_tag="O2701_002484"
     CDS             197..559
                     /locus_tag="O2701_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YojF family protein"
                     /protein_id="HCZ4294351.1"
                     /translation="MLEPIKEQEVIDLLASFEHKPVYLHVETTNGAYANHFDQRVFNA
                     GTFLRNIQITYTHAQLKGGNKEPYRIGLKLSNGGWVYVQGLTHFEVNEHDEFLIAGFN
                     YEGQLAAALQISERPFNL"
     gene            573..1238
                     /gene="bshB2"
                     /locus_tag="O2701_002485"
     CDS             573..1238
                     /gene="bshB2"
                     /locus_tag="O2701_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB2"
                     /protein_id="HCZ4294352.1"
                     /translation="MTDERHVLVIFPHPDDETFSSAGTLANYIQKGIPVTYACLTLGQ
                     MGRNLGNPPFATRESLPSIRERELEEACKVIGITDLRKMGLRDKTVEFEPYEHIDGMI
                     KSLIDDTNPSLIISFYPGYAVHPDHEATADAVIRTVERMPKEERPRLTLVAFSNDATE
                     ALGEPDIQNDITDFKELKIKAFEAHASQTGPFLKQLASPEIDGKQHSFLTVEPYWTYH
                     FKS"
     gene            1251..2129
                     /locus_tag="O2701_002486"
     CDS             1251..2129
                     /locus_tag="O2701_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HCZ4294353.1"
                     /translation="MTEFDLSTREGRWKHFGSVDPIEGTKPTTKNEMTDLQSTHKDFL
                     FEIEEVGIKNLVYPVLVDQYQTAGTFSFSTSLTKDEKGINMSRIIESVEKHYDNGIEL
                     EFNTLYQVLRTLQTNMKQNAAGVDVSGKWFFDRYSPTTNIKAVGNADVTYGLAIDGDK
                     VTRKELTIEATVTTLCPCSKEISEYSAHNQRGVVTVKTYINKDQDIVDDYKNKILDAM
                     EANASSILYPILKRPDEKRVTERAYENPRFVEDLIRLIAADLVEFDWLDGFDIECRNE
                     ESIHQHDAFAKLKYRK"
     gene            2411..3901
                     /locus_tag="O2701_002487"
     CDS             2411..3901
                     /locus_tag="O2701_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCZ4294354.1"
                     /translation="MNYFVGNSLGVNLTGIEKAIINRLNLFKEMGRPAQCVFLSWNRY
                     LYRNAQNYITSSDYINMYDFFQEATYLERNEPFDWLSYWTDECHYTLKHVENSHDFRI
                     YDQERFLMYAHFQDPKYRILDYVNHFDSQRRKVKRDFYDVRGFLSCSRILVDKQQTLC
                     EFFYNPEGDTKLEKYFSYKDGKPEVQKIIVYYANKQYFFNNETELGAFFIKQLYQHGD
                     LFFSDRNVYTAPIFNLTPESIPVVAVLHSTHIKNIDALDSSPFKNVYKAMFENLSRYR
                     AIIVSTEQQKLDVEKRINHTIPVVNIPVGYSETIDTPVQTLDQRSVKLISVARYSPEK
                     QLHQQIELIKRLVSYVPKIELHMYGFGSESKKLNELIQKYGLENHVYLRGFLSNLDQE
                     YSDAYLSLITSNMEGFSLALLESLAHGVPVISYDIKYGPNELITSDFNGYLITKNDED
                     ALFDKVKYVIDHPEVQQRLSKGSLAKAQQYSKASLIKQWDQFVRLI"
     gene            complement(4025..5497)
                     /locus_tag="O2701_002488"
     CDS             complement(4025..5497)
                     /locus_tag="O2701_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCZ4294355.1"
                     /translation="MNYILGTILESKITGVEKAQINRLKLFKQHGISSKCVYVKWNPY
                     SYTYAKQHQIENDVFTMYDYFQKAINYKKTKQVNWIQYWEKSCRYTLKFVENSNDVRI
                     YDEEQFVMYAHFLDKQYHQLNYVNYFDHKRRKVKRELYDGRGFLSCSRILGEGQRIVL
                     ENYYTPNGEIVIQKYFDDIKGKNTLTKVILNEDQHQQFFDTEDELVQYFLHQLCKNND
                     QIILDRPHELGNVIAGLNQSIPVIVVLHSTHLSGAGNGIKSFYKTVFNNLTRYKAIVV
                     STEKQCQDISQYIENKIPVINIPVGYVANLKYQFDINQKEKNHIISIARLVENKQIKH
                     QIEVIKQLVTKHPNIQLNIYGHGNGLSEYRQLVEDYHLSEHVKFHGFKTHINEEIAKA
                     ELMLSTSKMEGFGLAILESLSVGTPVISYDVDYGPSELIQDGFNGYLVPQGDINQMVE
                     KVDQLLNNTQKLQQFSINSIESAQQYNATTISTKWQNILN"
     gene            complement(5616..>5953)
                     /locus_tag="O2701_002489"
     CDS             complement(5616..>5953)
                     /locus_tag="O2701_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCZ4294356.1"
                     /translation="DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDAGKHTPVKPMSTTKDHHNKAKALPETGSENNGSNNATLFGGLFAALGSLLL
                     FGRRKKQNK"
BASE COUNT         1990 a          937 c          971 g         2055 t
ORIGIN      
        1 taatgtttaa cacttttcaa attccaacaa atttttgatt tttaaaagta aaaaactaat
       61 gctgtatgat tacgtaatcc tgagtaatta cccttcatga aaattaattt ataaataatt
      121 gagcaatcgt atacaattat cgaatagttt ttagtaaaat agaaagataa acacattgat
      181 agtgtaagga gtgaacgtgt tggaaccaat taaagaacaa gaagttatag atttacttgc
      241 ttcttttgaa cataaacctg tgtatctgca tgttgaaacg acaaatgggg cctatgcgaa
      301 ccatttcgat caacgtgttt ttaatgctgg tactttttta agaaacatac aaataacata
      361 tacgcatgca caactaaaag gtggtaataa agaaccttat cgcattggtc ttaaactatc
      421 aaatggtggt tgggtttatg tacaaggtct aacgcatttt gaggtcaatg agcatgatga
      481 atttttaatt gcaggtttta attacgaagg tcaactcgct gcagcacttc aaattagtga
      541 gcgaccattt aatttataga ggaggcatca acatgactga cgaaagacat gtattagtaa
      601 tatttccaca tcctgatgat gaaaccttct catctgcagg cacattagca aattatatcc
      661 aaaaaggcat acctgtaaca tatgcatgtt taaccctagg acaaatgggc agaaatttag
      721 gtaatccccc atttgcaaca cgcgaatcat taccaagtat tcgtgagcgt gaactagaag
      781 aagcttgtaa agttatcggt attactgatt taagaaaaat gggtttacgt gacaaaactg
      841 tcgaatttga accctatgaa catatagatg gtatgattaa atctttaatc gatgatacaa
      901 atccatcatt gattatttca ttttatcctg gatatgcagt acatcctgat cacgaagcaa
      961 cagcagacgc tgtaattcga acggttgaac gcatgcctaa agaagaacgc cctcgtttaa
     1021 ctttagttgc gttcagtaat gatgcaaccg aagctcttgg agaacctgat attcaaaatg
     1081 atattacaga ttttaaagaa cttaaaatta aggcatttga agcacatgct tctcaaacag
     1141 gtccattttt aaaacaatta gcaagtcctg aaatcgatgg taaacaacac agctttttaa
     1201 cagtcgaacc atattggaca tatcacttta aatcttaacg gaggaaaaaa atgactgaat
     1261 ttgatttatc gacacgagaa ggtcgttgga aacactttgg ttccgtagat cctattgaag
     1321 gcacgaaacc tactactaag aacgaaatga cagatttaca aagtacacat aaagattttt
     1381 tatttgaaat agaagaagtt ggtattaaaa acttagtgta ccctgtactt gttgaccagt
     1441 atcaaacagc tggcacattt agtttttcaa caagtttaac aaaagacgaa aaaggtatta
     1501 atatgagccg tataatcgaa agtgttgaaa agcactatga taatggtatt gaacttgaat
     1561 ttaatacact ttatcaggtg ttacgcactt tacaaacgaa tatgaaacaa aatgcggcag
     1621 gcgttgatgt ttctgggaaa tggttctttg atcgctatag tccaaccaca aatattaaag
     1681 cagttggtaa tgccgatgtc acttatggat tagcaattga cggtgataaa gttacacgta
     1741 aagaacttac tattgaagca actgtcacaa cgctatgccc ttgttcaaaa gaaataagtg
     1801 agtattcagc gcacaaccaa cgtggtgttg tcactgttaa aacatatatt aataaagatc
     1861 aagacatagt tgatgactat aaaaataaaa ttttagatgc aatggaagct aatgcaagtt
     1921 caattctata ccctatttta aaacgtcctg atgaaaaacg tgtgacagag cgtgcatatg
     1981 aaaatccacg ttttgtcgaa gacttaattc gtttaattgc cgcggactta gttgaatttg
     2041 attggttaga cggtttcgat atcgaatgtc gtaatgaaga gtctatccat caacatgatg
     2101 ctttcgctaa attaaagtat cgcaaataat atcatatgat taattcaaac gcgtaacatt
     2161 agttgcgcgt ttttattata ccttctagag ttgtatatct cttttataaa tcttctcatt
     2221 tgatgtgcct gagtcataaa tcaatatcct aagctctaca atgatatatt ggtcgtagtt
     2281 gattgaataa ctactatcat tataaatatc aaaatttgtt gttttataaa taaacacatc
     2341 ttccagttaa aagaagtaat taaatattat gtgatataat tataaaaaat agatataagt
     2401 ggtgttgtaa atgaattatt ttgtaggtaa tagtttaggt gttaatttaa ctgggataga
     2461 gaaagcaatt attaatcgtt taaatttatt taaagaaatg ggaagaccag cgcaatgtgt
     2521 atttctgtca tggaatagat atttatatag aaatgcccaa aattatatta ctagttctga
     2581 ctatattaat atgtatgatt tcttccaaga agcaacatat ttagaacgca atgaaccatt
     2641 tgactggttg tcgtattgga ctgatgaatg tcattatact ttaaaacatg ttgaaaatag
     2701 tcatgacttt agaatatatg atcaagagcg tttcttaatg tatgcccatt ttcaagatcc
     2761 taaatatcgt atattagatt atgtaaatca ttttgatagt caaagacgaa aagtaaaacg
     2821 tgatttttat gatgtgcgtg gctttttaag ttgtagtagg attcttgttg ataaacaaca
     2881 aacattatgt gaatttttct ataatccaga aggtgacaca aaattagaaa agtatttttc
     2941 ttacaaggat ggcaaaccag aagtacaaaa aattattgtt tactatgcta acaaacaata
     3001 tttctttaat aatgaaacgg aattaggcgc atttttcatt aaacaactat atcaacatgg
     3061 ggatttattt ttcagtgatc gtaatgtgta cacagcacct attttcaact taacgcctga
     3121 gtcaatccca gtagtagctg tgcttcacag tacacacatt aaaaatattg atgcattaga
     3181 ttcatcgcca tttaaaaatg tatataaagc gatgtttgaa aatttatctc gatatcgtgc
     3241 aattatcgtt tctactgagc agcaaaaatt agatgttgaa aaacgtatta accatactat
     3301 tccagttgtt aatattcctg tcggctatag tgaaacaatt gatacaccag tccaaacatt
     3361 agatcagcgc agtgttaaat taatttccgt tgctcgttat tcacctgaaa aacaattaca
     3421 tcaacaaatt gaattaatta aacgcttagt atcgtatgtc ccaaaaatag agcttcatat
     3481 gtatggtttt ggttctgaaa gtaaaaaatt aaatgaattg atacaaaaat atggattgga
     3541 aaatcatgtt tacttacgcg gctttttaag caatctagac caagaatata gcgatgccta
     3601 tttgagttta ataacaagta acatggaagg tttctcttta gcgctacttg aatctttagc
     3661 acatggtgtt ccagttatta gttatgatat taaatatgga cctaacgaac tgattacttc
     3721 agatttcaat ggttatttaa ttactaaaaa tgatgaagat gccctatttg ataaagtaaa
     3781 atatgtgatt gatcatcctg aagtacagca acgactgtca aaaggtagtt tggcaaaagc
     3841 acaacaatat agtaaagcgt cgttaattaa acaatgggac caatttgttc gtcttatata
     3901 aatgtacttt acctttattt ttacaaaaat agcattttcc tatgtcattt aactaaacat
     3961 gtaagttcgt atgaacgagg tttgttaaat agatgattct aggaaaatgc ttttttcttt
     4021 tgacttagtt taaaatattt tgccactttg tactgatagt agttgcattg tactgttgtg
     4081 cagattctat gctattaatt gaaaactgtt gcaatttttg agtattattt agtaattggt
     4141 cgaccttttc aaccatttga ttgatgtcac cttgaggtac taaatagcca ttaaatccat
     4201 cttgaatcag ttctgatgga ccataatcta catcataact gatcactggt gtacctactg
     4261 aaagcgactc taaaattgct aagccaaaac cttccatttt acttgtcgat aacatcagtt
     4321 ctgctttagc aatctcttca ttaatatgcg tcttaaaacc atgaaattta acatgttccg
     4381 ataaatgata atcttctaca agttgtcgat attctgacaa accatttcca tgtccataaa
     4441 tattcaattg aatattggga tgttttgtta ctaattgctt gattacttca atttgatgtt
     4501 taatttgttt attttcaacg aggcgagcaa ttgatatgat atgatttttc tccttttgat
     4561 tgatgtcaaa ttgatacttt aaatttgcca cgtagccaac cggaatattg ataactggta
     4621 ttttattttc aatatattgt gaaatatctt ggcattgctt ttctgttgat acaacaatcg
     4681 ctttataacg tgttaaatta ttaaacactg ttttataaaa actttttata ccattaccgg
     4741 caccggataa atgtgtacta tggagtacaa caataactgg aatactttga tttaatcccg
     4801 ctataacatt tcctaattca tgaggacgat ctaatatgat ttgatcatta tttttacata
     4861 attgatggag aaaatattga actaattcat cttctgtatc aaaaaattgt tgatgctggt
     4921 cttcatttaa gataaccttt gtgagcgtgt ttttcccttt tatatcgtca aaatattttt
     4981 ggatgacgat ttccccatta ggtgtatagt aattttcgag tacaatccgt tgtccttcac
     5041 ctaaaattcg agaacaactt aaaaagcctc ttccatcata caattcgcgt tttacttttc
     5101 ttcttttatg atcaaaataa ttcacataat ttaattgatg atactgttta tctaaaaaat
     5161 gagcatacat tacaaattgc tcttcatcat atattctgac atcatttgaa ttttccacaa
     5221 atttcaatgt gtacctacat gacttttccc aatactgtat ccagttaact tgctttgtct
     5281 ttttataatt gattgctttt tgaaaatagt catacattgt aaatacatca ttttcaatct
     5341 gatgttgctt cgcatatgtg tatgaataag gattccattt aacatataca cattttgaag
     5401 atatgccgtg ttgtttgaac aacttcaatc tatttatttg cgctttttct acacctgtaa
     5461 ttttactttc taaaattgtt cctaaaatgt aattcatatt atcgcctcat ataagtttta
     5521 ttccgtatct ttattgttta ttttatatga aaaatacatc tattgcatgt gtaattataa
     5581 aaaaaccagg ccacaaggac ctgggtcata ttgtattatt tgttttgttt tttgcgacga
     5641 ccgaataaca ataatgaacc taatgctgca aataatccac caaataacgt tgcgttattt
     5701 gagccgttat tttcactacc tgtttctggt aatgcttttg ctttattgtg atggtcttta
     5761 gtagtactca ttggtttaac aggtgtatgt tttcctgcat ccgagtctga atcgctgtct
     5821 gaatcactgt ctgagtctga gtcgctatca gagtctgagt cgctgtccga atctgagtcg
     5881 ctatctgagt ctgagtcgct gtctgaatct gaatcactgt ctgagtctga gtcgctatct
     5941 gagtctgaat cgc
//
LOCUS       DALAGD010000128         5735 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.131, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000128 DALAGD010000000
VERSION     DALAGD010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.131"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..510
                     /gene="sek"
                     /locus_tag="O2701_002490"
     CDS             <1..510
                     /gene="sek"
                     /locus_tag="O2701_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type K"
                     /protein_id="HCZ4294357.1"
                     /translation="FNKFSNFKGKKLDVFGISYNGQCNNKYIYGGVTATNEYLDKPRN
                     IPINIWINGNHKTISTNKVSTNKKFVTAQEIDVKLRKYLQEEYNIYGHNGTKKGEEYG
                     HKSKFYSGFNIGKVTFHLNNNDTFSYDLFYTGDDGLPKSFLKIYEDNKTVESEKFHLD
                     VDISYKETI"
     gene            598..1818
                     /locus_tag="O2701_002491"
     CDS             598..1818
                     /locus_tag="O2701_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCZ4294358.1"
                     /translation="MWLEKFKNKNKETKYRYYEKYKDPLTAKWRRVSVVLNKNGKQSQ
                     KEAQRLLNERIEEKLNDKKPTTLKSLTFHVACDEWFQNYIKTSGSKRTTIKTKLSKLN
                     TLKKFVDEDILINKITLSYAQQVFDEMDSKGYVYQVNKDALSIFKNVFEYTRRIYKLQ
                     DLEFLKDITLNKRIKSYDEVKAKRNKYLELNEIESIIKDINTKAQKMHSGIHKRFYLF
                     VALMTEFQALNGMRIGEMLAIQNEDIDFDNKSLNINGTIHWFHDESGGFGVKDTTKTE
                     SSYRTIGLSSRSCEILKKAILENKKDSKWNDGYLNRNFVFTNHKGNPMQTERFNKILR
                     EAAKDVGIDKEVSSHILRHSHISLLSQQGVSLKAIMDRVGHSDHRTTLSIYSHVTEQM
                     DKDMMNKLEQVKLG"
     gene            complement(1960..2781)
                     /locus_tag="O2701_002492"
     CDS             complement(1960..2781)
                     /locus_tag="O2701_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCZ4294359.1"
                     /translation="MKKLFGLILVLTFAVVLAACGNGNKSGSDDKKITVGASPAPHAE
                     ILEKAKPLLEKKGYELDIKTINDYTTPNKLLDKGEIDANYFQHTPYLNTEKKDKGYKI
                     VSAGDVHLEPMAVYSKKYKSLKELPKGATVYVSNNPAEQGRFLKFFVDAGLIKIKKGV
                     KIEDAKFSDITENKKDIKFNNKQSAEFLPKIYQNEDADAVIINSNFAIEQKLNPKKDS
                     IAVESAKDNPYANLIAVKEGHQDDKKIKALIEVLQSKDIQDFINEKYNGAVIPAK"
     gene            complement(2799..3494)
                     /locus_tag="O2701_002493"
     CDS             complement(2799..3494)
                     /locus_tag="O2701_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4294360.1"
                     /translation="MGKSFSEIINEMITMPNIQWPEVWTAIVETLYMTVVSTIFAFIL
                     GLILGVLLFLSAKGKSIGARLFYSIVSFIVNLFRAIPFIILILLLIPFTSLILGTISG
                     PTGALPALIIGAAPFYARLVEIAFKEIDKGVIEAAWSMGANTWTVIRKVLLPEAMPAL
                     VSGITVTAIALVGSTAVAGVIGAGGLGNLAYLTGFTRNQNDVILVSTVFILIIVFIIQ
                     FIGDWLTNKLDKR"
     gene            complement(3487..4512)
                     /locus_tag="O2701_002494"
     CDS             complement(3487..4512)
                     /locus_tag="O2701_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HCZ4294361.1"
                     /translation="MIELKEVVKEYRTKNKEVLAVDHVNLSIRAGSIYGVIGFSGAGK
                     STLIRMFNHLEAPTSGEVIIDGDHIGQLSKNGLRAKRQKVSMIFQHFNLLWSRTVLKN
                     IMFPLEIAGVPRRRAKQKALELVELVGLKGREKAYPSELSGGQKQRVGIARALANDPT
                     VLLCDEATSALDPQTTDEILDLLLKIREQQNLTIVLITHEMHVIRRICDEVAVMESGK
                     VIEQGPVTQVFENPQHTVTKRFVKDDLNDDFETSLTELEPLEKDAYIVRLVFAGSTTT
                     EPIVSSLSTAYDIKINILEANIKNTKNGTVGFLVLHIPYISSVDFGKFEKELIERQVK
                     MEVLRHG"
     regulatory      complement(4601..4704)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4762..5058)
                     /locus_tag="O2701_002495"
     CDS             complement(4762..5058)
                     /locus_tag="O2701_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCZ4294362.1"
                     /translation="MNNSLDIKDVTTFYEEDKHLIFGYTPTCGTCKVSERMLDIANEI
                     LQLPLLKIDLNFYPQFCKDMQIMSTPILLLMNKDKEVKRIYAFKSVTDLLENLK"
     gene            complement(5051..5437)
                     /locus_tag="O2701_002496"
     CDS             complement(5051..5437)
                     /locus_tag="O2701_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCZ4294363.1"
                     /translation="MAIVNKVIIVEGKSDKKRVQQVIAEPVNIICTHGTMSIDKLDDM
                     IESLYDKQVFVLADSDDEGDRIRNWFKRYLSESEHIFIDKTYCQVANCPKQYLAHVLS
                     KHGFTCKKETPLLPNINNERLVLVNE"
BASE COUNT         1984 a          982 c          839 g         1930 t
ORIGIN      
        1 tttaataaat ttagtaattt caagggaaaa aaacttgatg tttttggtat tagttataat
       61 ggccagtgta acaataaata catatatggt ggagtcacag ctactaacga atatctagat
      121 aaacctagaa atatccctat aaatatatgg atcaatggaa atcacaaaac tatttctacc
      181 aataaagttt cgacaaataa aaaatttgtt accgctcaag agattgatgt caaattaaga
      241 aagtaccttc aagaagaata caacatttat ggacataacg gcactaaaaa aggagaagaa
      301 tatggtcata aatcaaaatt ttattctggg tttaatattg gtaaagtaac gttccattta
      361 aataataatg acactttttc atatgattta ttctacacag gagatgatgg gttaccaaaa
      421 agttttttaa aaatttacga agacaataaa actgtagagt ctgagaaatt ccatttggat
      481 gtcgatattt cttataaaga aacgatataa atctattaat tatgtcataa tcattatttt
      541 ccgggtagca tgcctaccct tattattttt tgccaatttt gaggagggag aagcaaaatg
      601 tggttagaaa aatttaaaaa taagaataaa gaaactaaat atagatatta cgagaagtac
      661 aaagaccctc tcactgccaa atggagacgt gttagcgtgg tacttaataa gaatggtaag
      721 caatcacaaa aagaagctca aagactctta aatgagcgta tagaagaaaa actgaatgat
      781 aagaaaccta ctacacttaa gtcactaact ttccatgtcg catgtgatga gtggtttcag
      841 aattatatca aaacgtctgg gtcaaaaaga acaactatta aaactaaatt gagtaagcta
      901 aacactttga agaaatttgt agatgaagat attttaatca ataaaataac actttcatat
      961 gcgcaacaag ttttcgatga aatggatagt aaaggttatg tatatcaagt aaacaaagat
     1021 gcgttaagca tattcaaaaa tgtatttgaa tacactagac gtatctataa actgcaagat
     1081 ttagaatttt taaaagatat aacattaaat aaaagaatta agtcttatga tgaagtgaaa
     1141 gctaaacgta ataagtacct cgaattaaat gaaatagaat ctatcatcaa agatattaat
     1201 acgaaggctc agaagatgca ctcaggtatc cataagcggt tttatttatt cgttgcactc
     1261 atgacagaat tccaagcctt aaatggtatg cgtattggtg aaatgttagc cattcaaaat
     1321 gaagatatag actttgataa taagagttta aatattaacg gaacaatcca ttggtttcac
     1381 gatgaatctg gtggattcgg tgtaaaagat accactaaaa cagagtctag ttatagaaca
     1441 attggattga gtagcagaag ttgcgagata ttaaagaaag caatactgga gaacaaaaag
     1501 gattcaaaat ggaatgatgg atatctaaat agaaattttg tatttactaa tcataaaggc
     1561 aacccaatgc agactgaaag attcaataaa atccttaggg aagcagctaa agatgttggt
     1621 attgataaag aagtttcttc acatatatta agacatagcc acatatcatt actctctcaa
     1681 caaggcgtgt cacttaaagc tattatggat cgtgtaggcc attccgacca ccgtacaaca
     1741 ctttccattt attctcacgt tactgaacag atggataagg atatgatgaa caaacttgaa
     1801 caggtaaaac ttggataatg cgctcaaatc tgagcgtatt tttttgttta aaattcgata
     1861 atttgcatat actgcccctt ttgtgccctt ttttattttc aataccacaa aaatacccct
     1921 taatcgttgt tattaaaggg tatttttatt taattggtat tatttagcag gaataactgc
     1981 accattgtat ttttcattaa tgaagtcttg aatatcttta gattgtaata cttcaattaa
     2041 tgctttgatt ttcttatcat cttgatgtcc ttctttaaca gcaattaagt ttgcataagg
     2101 attatctttc gcactttcta cagcaataga atctttttta ggatttagtt tttgttcgat
     2161 tgcaaagttc gaattaatga taacagcatc agcgtcttca ttttgataaa ttttaggtaa
     2221 gaattctgct gattgtttat tattaaactt aatatctttt ttattctctg taatatcact
     2281 aaacttagca tcttcaattt ttacgccttt tttgatttta attaaacctg catcaacgaa
     2341 gaattttaag aaacgtcctt gttcagctgg attattagac acatagactg ttgcaccttt
     2401 tggtaattct tttaaacttt tatacttttt agagtataca gccataggtt ctaagtgaac
     2461 atcaccggca cttacgattt tgtaaccttt atcctttttc tctgtgttta aatatggtgt
     2521 atgttggaaa tagtttgcgt caatttcacc tttgtctagt aatttattag gtgtagtgta
     2581 atcgttaatt gttttaatat ctagttcata accttttttc tctaataatg gttttgcttt
     2641 ttctaaaatt tcagcatgtg gtgctggtga agcacctact gttattttct tgtcgtcact
     2701 gccacttttg tttccattac cgcaagctgc taatacaact gcaaatgtta atactaaaat
     2761 aagaccaaat aattttttca taaaatgaaa cccccaattt atcgtttatc aagtttattt
     2821 gtaagccaat ccccaatgaa ttggattata aatacaataa ttaaaataaa aactgttgat
     2881 actaaaatga catcattttg atttcgagtg aaacctgtta agtatgctaa atttcctaaa
     2941 ccaccggcac caattacacc tgcaactgct gttgaaccaa ctaaagcgat tgctgtaact
     3001 gtaatgccag acactagcgc tggcatagct tcaggtaaaa ggactttacg aattactgtc
     3061 caagtattag cgcccattga ccaagccgct tcgatgacac ctttatcaat ttctttaaaa
     3121 gcaatttcta cgagccttgc ataaaacggt gctgcgccaa tgatcaaggc tggtaacgca
     3181 cctgtcggac cacttatcgt tccaagtatc aaacttgtaa atggaattaa taataaaatt
     3241 aaaataataa atggtatcgc tctaaataag ttaacaatga aagaaacgat agaataaaat
     3301 aaccttgcac cgatagactt acctttagca gacaagaata ataacacacc taaaataaga
     3361 ccaagtataa atgcaaatat agttgagacg actgtcatgt atagtgtttc gactattgca
     3421 gtccaaactt ctggccactg aatattaggc attgtaatca tttcatttat aatttcacta
     3481 aatgatttac ccatgtctta acacctccat tttaacttgt cgctcaatta actctttttc
     3541 gaattttccg aaatctacac ttgaaatata tggaatatgc agaactaaaa agccgactgt
     3601 tccatttttt gtatttttaa tatttgcttc taaaatatta attttaatat cataggcagt
     3661 tgatagactc gatacaatag gctcggttgt tgttgaacca gcgaaaacta atctaacgat
     3721 atatgcatct ttttctaatg gctctaattc tgttaaagat gtttcgaaat catcatttaa
     3781 atcgtctttc acaaatcgtt ttgtcacagt gtgttgcgga ttttcaaaaa cctgtgtcac
     3841 cggtccttgt tctatcactt taccactttc cataactgca acttcatcac aaatacgacg
     3901 aatgacatgc atttcatgcg taattagtac aattgttaaa ttttgttgtt ctctaatttt
     3961 tagtagtaga tctaaaattt catctgttgt ttgcggatca agtgcacttg ttgcctcatc
     4021 acaaagcaag accgttggat cattagctaa cgctcgtgca atcccaacac gttgcttttg
     4081 tccacctgat aactctgatg gataagcctt ttctctacct tttaaaccga cgagttcgac
     4141 aagttctaat gctttttgct tagctctcct tctagggaca cctgcaattt caagcggaaa
     4201 cataatattt tttaacacag tccttgacca taacaaatta aaatgttgga agatcatact
     4261 tactttttgt ctttttgctc ttaatccatt tttggacaat tgacctatat ggtctccatc
     4321 tataataact tcacctgatg taggcgcttc taaatgatta aacattcgaa tcaaagtact
     4381 ttttcctgct ccagaaaaac caatgacgcc ataaatcgat cctgctcgaa tcgataaatt
     4441 aacgtgatct acagcaagga cttctttatt tttagtccga tattctttaa caacttcttt
     4501 taattcaatc acgttgattc ctccctgtgt tgcttaataa aataaaaaat gctttctcaa
     4561 tatcgataga aaaattgaga aagcaatagt agtattgttt ctctcatctt caaaagttaa
     4621 aactttatgt gaattggcac catttctata taagacggtt gccgggcttc gtagggcaca
     4681 tccctccacc actctcgata agagtttacg catcatttaa tttgtattaa tcctaacacc
     4741 ttagtaaaat ttcgtcaata actattttaa attttctaac aaatcagtca ccgatttaaa
     4801 tgcataaatt cgttttactt ctttatcttt attcatcaac aataaaatcg gcgtagacat
     4861 gatttgcata tctttacaaa actgaggata aaagtttaaa tctattttca ataatggtaa
     4921 ctgcaatatt tcattagcaa tgtctaacat tctttctgaa accttacaag taccacacgt
     4981 tggtgtataa ccaaagatta aatgtttgtc ttcctcataa aatgtagtta catctttgat
     5041 gtctaatgaa ttattcattt actaaaacta acctttcatt atttatattc ggtaaaagag
     5101 gtgtttcttt cttacaagta aagccatgtt ttgaaagtac atgcgccaaa tattgtttgg
     5161 ggcaattcgc aacttgacag taagttttat caataaatat atgttcactt tcactcaaat
     5221 aacgtttaaa ccaatttcta attcgatctc cttcgtcatc agaatcggct aatacaaaaa
     5281 cttgtttatc atacagtgat tctatcatat catcaagctt atctatactc attgttccat
     5341 gagtacaaat aatattgact ggttctgcaa taacctgttg cacccttttt ttatcagatt
     5401 ttccttcaac aattatcact ttatttacaa tagccatcat catcaccctt taaaatcaat
     5461 aaacatctgt cactgtatca tttcacaaaa ttggtatgaa taaaacataa atcacaaaaa
     5521 atttaaacta gcttaatata ataattacaa actcaatgtt tgactagctg gaacatttaa
     5581 cataagcaga caaaggctaa gtcaaaaatc aacatcctaa aatctacaat gttatattaa
     5641 caatagttaa ccaaaagaaa atacacctat aacaaacttt tcaattatag cggggcccca
     5701 acacagaagc tgatggtaag tcagcttaca ataat
//
LOCUS       DALAGD010000129         5619 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.132, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000129 DALAGD010000000
VERSION     DALAGD010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5619
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.132"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(45..611)
                     /locus_tag="O2701_002497"
     CDS             complement(45..611)
                     /locus_tag="O2701_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HCZ4294364.1"
                     /translation="MLCENCQLNEAELKVKVTSKNKTEEKMVCQTCAEGHHPWNQANE
                     QPEYQEHQDNFEEAFVVKQILQHLATKHGINFQEVAFKEEKRCPSCHMTLKDIAHVGK
                     FGCANCYATFKDDIIDIVRRVQGGQFEHVGKTPHSSHKKIALKRKIEEKNEYLKKLIE
                     IQDFEEAAIVRDEIKALKAESEVQHDDA"
     gene            complement(630..1091)
                     /locus_tag="O2701_002498"
     CDS             complement(630..1091)
                     /locus_tag="O2701_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HCZ4294365.1"
                     /translation="MHNMSDIIEQYIKRLFEESNEDVVEIQRANIAQRFDCVPSQLNY
                     VIKTRFTNEHGYEIESKRGGGGYIRITKIENKDATGYINHLLQLIGPSISQQQAYYII
                     DGLLDKMLINEREAKMIQAVIDRETLSMDMVSRDIIRANILKRLLPVINYY"
     gene            1249..2463
                     /locus_tag="O2701_002499"
     CDS             1249..2463
                     /locus_tag="O2701_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCZ4294366.1"
                     /translation="MHILIGIIGIIFFLALAFLFSSDRKNIRWKYVGILLAIQLIFAF
                     ILLKTTMGIKVIGGISNGFNYLLLKAADGVNFVFGGIQYIDPKQPPFFFSVLLPIVFI
                     SAIIGILQYTKILPLIINVLGFLISKINGMGRLESYNAVAAAILGQSEVFISIKKQLP
                     YIPKQRLYTLTASAMSTVSASIIGAYFTLIEPKYVVTAVVLNLFGGFIIASIINPYKV
                     NEEDDKLLVEETEERQQSFFEMLGEYILDGFKVAVIVGAMLIGYIAIIALLNGIVSNI
                     FSTVSGGAISWDFQTLIGFVFAPFAFLVGVPWQDAVQAGSVMATKLLSNEFVAMQALG
                     KLSDLSEHAKGVTSVFLVSFANFSSIGIISGAIKSLNDKKGDTVARFGLKLLFGATLV
                     SFISAAIAGFFI"
     gene            complement(2671..3231)
                     /gene="pdxT"
                     /locus_tag="O2701_002500"
     CDS             complement(2671..3231)
                     /gene="pdxT"
                     /locus_tag="O2701_002500"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HCZ4294367.1"
                     /translation="MKIGVLALQGAVREHIRHIELSGHEGIAVKKVEQLEEIEGLILP
                     GGESTTLRRLMNLYGFKEALQNSTLPMFGTCAGLIVLAQDIVGEEGYLNKLNITVQRN
                     SFGRQVDSFETELDIKGIATDIEGVFIRAPHIEKVGQGVDILCKVNEKIVAVQQGKYL
                     GVSFHPELTDDYRVTDYFINHIVKKA"
     gene            complement(3235..4122)
                     /gene="pdxS"
                     /locus_tag="O2701_002501"
     CDS             complement(3235..4122)
                     /gene="pdxS"
                     /locus_tag="O2701_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HCZ4294368.1"
                     /translation="MSKIIGSDRVKRGMAEMQKGGVIMDVVNAEQARIAEEAGAVAVM
                     ALERVPSDIRAAGGVARMANPKIVEEVMNAVSIPVMAKARIGHITEARVLEAMGVDYI
                     DESEVLTPADEEYHLRKDQFTVPFVCGCRNLGEAARRIGEGAAMLRTKGEPGTGNIVE
                     AVRHMRQVNSEVSRLTVMNDDEIMTFAKDIGAPYEILKQIKDNGRLPVVNFAAGGVAT
                     PQDAALMMELGADGVFVGSGIFKSEDPEKFAKAIVQATTHYQDYELIGRLASELGTAM
                     KGLDINQLSLEERMQERGW"
     gene            4226..5608
                     /locus_tag="O2701_002502"
     CDS             4226..5608
                     /locus_tag="O2701_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCZ4294369.1"
                     /translation="MKNTLYHQLYEKLKKQIIEGQFKEGDKFYSKRQLSKHLSISQTT
                     VEHAYQLLLDEGYIYSRPRSGYFVSEIESLTILNNQPIPSLFDDDSYKPKASDEAYDY
                     AFNLDEIDTKHFPIELFRKYSKDLYDTNHLNQLRRGHFQGELHLRFQLAFYLFTNRGV
                     ICDPNQIIIGSSTEQLVNQLVDLLYTSTFIIEKPSYPPIKNILDKKQVEYEQIEVEDN
                     GINVDEVIKSQKNIVYITPSHQFPTGYVMDLKKRTQLIQWAQEKEERFIIEDDYDSEF
                     RYFGKPIPAIQGLYSRGEKVIYISTFSKSIFPSCRVAYMVLPYSIMKKYHSQNHIEGN
                     TVPVHMQNLIATFISSGGFERHLNKMRRIYRRKLTYILKRLKPYKEQLDIQGAETGMH
                     FTITVKNGLTLQECLDRANKVKLKLQVYNFDDDQDYKKTPKFILGFGGIDDDALKPHV
                     DALIKSLIIK"
BASE COUNT         1805 a         1021 c          798 g         1995 t
ORIGIN      
        1 tttcttcatt acttttcatc cattggctga tattatcatg aatattatgc gtcatcatgt
       61 tgcacctcac tctcagcttt tagtgcttta atttcatctc taacaatggc tgcttcctca
      121 aaatcttgga tttcaataag ttttttcaaa tattcattct tttcttcgat ttttcgcttt
      181 aaagctatct ttttatgtga agaatgtggt gtctttccaa cgtgctcaaa ttgtccacct
      241 tgaactctgc ggacgatatc aatgatgtca tctttaaatg ttgcataaca attagcacac
      301 ccaaatttac caacatgtgc aatatctttc aaagtcatat gacatgatgg gcaacgtttt
      361 tcttctttaa acgctacttc ttgaaaatta attccatgtt tcgtagctaa atgttgtaaa
      421 atttgcttaa caacaaatgc ttcttcgaaa ttatcttgat gttcttgata ttcaggttgt
      481 tcattagctt gattccacgg atggtgtccc tcagcacaag tttgacacac cattttttct
      541 tctgttttat ttttacttgt aactttaact tttaattccg cttcattaag ttgacaattt
      601 tcacaaagca cttcaacacc tcatttcatt tagtaataat ttataactgg taacaaacgt
      661 tttaaaatat ttgctctaat aatatctcta gaaaccatat ccattgatag cgtttctcta
      721 tcaataactg cttgaatcat tttagcttca cgttcattta ttaacatttt atctaaaagc
      781 ccatcaataa tataataagc ttgttgttga gaaatagaag gtccaatcag ctgaagcaaa
      841 tgattaatat aacctgttgc atctttattt tcaattttag tgattcggat gtaaccacca
      901 ccaccacgtt tactttcgat ttcataacca tgttcattag tgaatcgtgt tttgattaca
      961 taatttaatt gtgatggtac gcaatcaaaa cgctgtgcga tattcgctct ctgaatttca
     1021 acgacatctt cattcgactc ttcaaataaa cgtttgatgt attgttctat gatgtcagac
     1081 atattgtgca tgtatatcac ccctttttga ccttctttga ctatattata tgacctattt
     1141 tgaccttttt caattaattt gtttttatat tttcaaaaat ttatgatgtt agcccttcca
     1201 ttttattgta tgatagattg tagaaaaaac taaaggaatg agaaatatat gcatattcta
     1261 attgggatta tcggaattat cttcttttta gcacttgcgt ttctatttag ctcagacagg
     1321 aaaaatattc gctggaaata tgtaggaatt ttacttgcaa ttcagcttat ctttgcattt
     1381 atcttgctta aaactacaat gggaatcaag gttattggtg gtatttcaaa cggttttaac
     1441 tacttattat taaaagcagc agatggtgtt aactttgtat tcggtggtat tcaatatatc
     1501 gatcctaagc aaccaccatt cttcttcagt gtattattac ctatcgtatt tatttcagcg
     1561 attatcggta tccttcaata cacaaagatt ttacctttaa tcattaatgt tttaggattc
     1621 ttaatttcta aaattaatgg tatgggacgt ttagaatcat acaatgcagt tgctgcagca
     1681 attttgggac aatctgaagt atttatttca attaaaaaac aattaccata catacctaag
     1741 caacgtttat acacattaac agcttcagca atgtcgactg tttctgcttc aattatcggt
     1801 gcatacttta ccttaattga acctaaatat gttgtaacgg ctgttgtctt aaacttattt
     1861 ggtggtttca tcatcgcttc aattattaac ccttataaag ttaatgaaga agatgacaaa
     1921 ttattagttg aagaaactga agaaagacaa caatcattct tcgaaatgct aggagaatat
     1981 attttagatg gatttaaagt agctgtcatt gtaggcgcaa tgttaatcgg atacattgcc
     2041 attatcgctc tattaaatgg aattgtaagc aatatcttta gtacagtttc aggtggcgct
     2101 atttcttggg atttccaaac attaattggt tttgtctttg caccatttgc attcttagtc
     2161 ggtgtgccat ggcaagatgc agttcaagcc ggttcagtta tggcgacaaa actattatct
     2221 aacgaatttg tagcaatgca agcacttggt aaacttagtg atttatctga acacgcaaaa
     2281 ggtgttacat ctgtattctt agtatcattt gctaacttta gctctatcgg tattatttca
     2341 ggtgcgatta aatcacttaa tgacaaaaaa ggagatacag ttgcccgctt tggtttgaaa
     2401 ttattattcg gtgcaacact agtatctttc atttcagctg caatcgctgg attctttatt
     2461 taacaaatac atcaacatcg aattaacccc ttcaaaattt tgactattct cagtctcttt
     2521 gaagggtttt tttcttatgt atagctttat tttttatcat ttttaatttt gacataaaaa
     2581 ataactcctc caaagctaag tttgaaggag atattttctt ctcaacaaat ctataaatat
     2641 caataattcg ttgatttagc atacattaag ctatgctttt tttacaatat gattaataaa
     2701 gtaatcagtt actctatagt catctgttaa ttcaggatgg aatgatacgc ctaaatattt
     2761 accttgctga acagctacaa ttttctcatt aaccttacat aggatatcta cgccttgacc
     2821 tactttttca atatgtgggg ctcttataaa gacaccttca atatctgtag cgataccttt
     2881 aatatctaat tctgtttcaa agctgtcaac ttgtctaccg aatgagtttc gttgtacagt
     2941 aatattcaac ttgttaaggt atccttcttc accaactata tcttgcgcta gaactattaa
     3001 tcctgcgcat gtaccaaaca taggtaaagt tgaattttgt aaagcctctt taaatccata
     3061 taaattcatt aatcgacgta acgttgtaga ctcgccacca ggtaatatta agccctcgat
     3121 ttcttctaat tgttcaactt ttttaactgc aataccttca tgaccactta attcaatatg
     3181 tctaatatgt tcacgtactg caccttgtaa tgctaataca cctattttca tatcttacca
     3241 accacgctct tgcatacgtt cttctaatga taattgattg atatctaaac ctttcatagc
     3301 agtgccaagt tcacttgcta atcttccaat tagttcatag tcttggtaat gtgttgttgc
     3361 ttgaacaatt gctttagcaa atttttctgg atcttctgat ttaaaaatac ctgatccaac
     3421 gaatacaccg tcagcaccta attccatcat taaagcagca tcttgaggag tcgcaacgcc
     3481 accagctgca aagttaacta ccggtaaacg accattgtct ttaatttgtt ttaaaatttc
     3541 ataaggcgca ccgatatctt tcgcaaaagt cataatctca tcatcattca ttacagtcaa
     3601 tcgactaact tctgaattaa cttgtctcat atgtcttaca gcttcaacaa tattacctgt
     3661 acctggttca cctttagtac gtaacatagc agcaccttca ccaattctac gcgcagcttc
     3721 acctaaatta cgacatccac atacaaatgg tactgtaaat tgatcttttc ttaagtgata
     3781 ttcctcatct gctggtgtta acacttctga ttcatcaata tagtcaacac ccatcgcctc
     3841 taatactctt gcttcagtga tatgaccaat acgtgcttta gccatgactg gaatagaaac
     3901 agcattcatt acttcttcta caattttagg gtttgccata cgtgcaacac caccagcagc
     3961 tctaatatca gaaggtactc gttctaatgc cataactgct accgcgccag cttcttctgc
     4021 aattcttgct tgctcagcat taacgacatc cataataacg ccgccttttt gcatttcagc
     4081 catacctctt ttgactctgt ctgatccaat aattttactc attatacata aaaccccttt
     4141 tcaagttgat taaatacatt gtaatgtata aactgatatt taaaaaggta caaaataaga
     4201 taaatttagg aggtcagtta taaaattgaa aaacacttta taccatcaac tttatgaaaa
     4261 attaaaaaaa caaataattg aaggtcaatt taaagaaggc gataaatttt attctaaaag
     4321 acaattaagc aaacatttat ctattagcca aacaaccgta gagcatgcat atcaacttct
     4381 attagacgaa ggttacattt attcaagacc tcgctctgga tattttgtgt ccgaaattga
     4441 atctttaaca attttaaaca atcaacctat cccctctctt tttgatgatg attcatataa
     4501 accaaaagcg agtgatgaag cttatgatta tgcttttaat ttggatgaaa tagatacgaa
     4561 acatttccct atagaacttt tcagaaagta ttccaaagat ctttatgata caaatcattt
     4621 aaatcaatta cgtagaggac attttcaagg agaattacat ttgcggtttc aactggcatt
     4681 ttatttattt actaatcgtg gcgtaatttg tgatcctaat caaattataa ttggctcatc
     4741 taccgaacaa ttagtaaatc aattagtcga tttactttat acttcaacat ttattatcga
     4801 aaaacctagt tatccaccaa tcaaaaatat tttagataaa aaacaagttg aatatgaaca
     4861 aattgaagtt gaagataatg gaataaacgt agatgaagtt attaaatcac aaaaaaatat
     4921 agtatatata acgccttccc atcaatttcc aactggatat gtgatggact taaaaaaacg
     4981 aactcaatta atacaatggg ctcaagaaaa agaagaacga tttattatag aagatgatta
     5041 tgattccgag tttagatatt tcggcaaacc cattccagca attcaaggtc tatattcaag
     5101 aggagaaaaa gtgatttata tcagtacatt ttcaaagtct atttttccta gctgtcgagt
     5161 tgcatatatg gtgttaccct attctataat gaaaaaatat cattctcaaa atcacattga
     5221 aggcaacact gtacctgttc atatgcaaaa ccttattgca acgtttatat cttccggtgg
     5281 ttttgaaagg catttaaata aaatgagacg aatttatcgt agaaaactaa cctatatttt
     5341 aaaaagattg aaaccttaca aagagcagct tgatattcaa ggtgctgaaa cgggtatgca
     5401 ttttacaatt acagtaaaaa atggattaac attacaagag tgtcttgata gagcaaacaa
     5461 agtaaagtta aaattacaag tttataattt tgatgatgat caagactata agaaaacccc
     5521 taaatttata ttaggctttg gcggtataga cgatgatgct ttaaaaccgc atgtagacgc
     5581 tctaataaaa tcattaatta taaagtaaca cttctttga
//
LOCUS       DALAGD010000130         5605 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.133, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000130 DALAGD010000000
VERSION     DALAGD010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5605
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.133"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            87..452
                     /locus_tag="O2701_002503"
     CDS             87..452
                     /locus_tag="O2701_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HCZ4294370.1"
                     /translation="MKKKKNAIKVIIILFISLIVAVAFFFGLKTYQGHKNIQLIDSYL
                     EEKNLKDKIKSEKTEYSAKKGLFYKEVTFKDEPGVTYVVQPISTNKGLFVEGFDTETK
                     KSLKTAKHKYFNQNYKPSK"
     gene            634..1911
                     /locus_tag="O2701_002504"
     CDS             634..1911
                     /locus_tag="O2701_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCZ4294371.1"
                     /translation="MALFKRKISLPMQVVIALVLGVVVGLLLYGQENVANYIKPFGDV
                     FLNLIKMIVIPVVFCSLALSISNVGESKTVGRYGWKTILYFEIITTIAIGLGIIFGNL
                     FKPGAGLDPTKLPKGDISKYQSTAHAAEQSTYGNHFIDTIVHIIPTNFFEALNKGELL
                     PIIFFAVFFGLGLAAVGKKAEPVKEFLSGSLEAVFWMINKILKLAPLGVFAFICTTII
                     TFGASALLPLLKLVLVVVFAMVFFVFAILGLVAWMCGINIMNIIRILKSELLLAFSTS
                     SSEAVLPVMMKKMENFGSPKEITSFVIPIGYTFNLDGSALYQSIAALFVAQMYGMHLT
                     LSEQIVLMLTLMITSKGMAAVPGTSIVVLLTTLGAMGLPAQGLALIIGVDRILDMVRT
                     CVNVIGNALSTIVIAKWENVYDKAKGQEYLKSI"
     gene            complement(2315..2671)
                     /locus_tag="O2701_002505"
     CDS             complement(2315..2671)
                     /locus_tag="O2701_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HCZ4294372.1"
                     /translation="MKKKKGFGLGISLIAIMLIVCIVLVIMMMTGGKKDTYYGIMKDN
                     TTIEKMISEKDESIEKNVKLPSDSDVKVKKGDFVIVYKLADSDKIVKVKKVDHDDVPH
                     GLMMKIHDMGKMHMKH"
     gene            complement(2936..3358)
                     /locus_tag="O2701_002506"
     CDS             complement(2936..3358)
                     /locus_tag="O2701_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HCZ4294373.1"
                     /translation="MSNSQAIQAIENVLATSKVGVLSTAYNNKPNSRYMVFYNDGLTL
                     YTKTNIHSAKVKEIKDNPAAYVLLGYNDTTNRSFVEMEATIEIVTEQEVIDWLWETQD
                     KSFFSSKEDPELCVLRVVPQSIKLMNDKSLDTPIKIDL"
     gene            complement(3429..5534)
                     /gene="rsp"
                     /locus_tag="O2701_002507"
     CDS             complement(3429..5534)
                     /gene="rsp"
                     /locus_tag="O2701_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator Rsp"
                     /protein_id="HCZ4294374.1"
                     /translation="MTCQLKIHHTLSTIPSRNINNIMVLFSLTSKLNITINGETKDVS
                     NYIILINHGDIYNINHGENIIELMIPVFYFYQQDDDFFNGYLDRHLLQSSNYIKSLIA
                     DLISTPTSSSLMGKNIGQSIIDTLLKEAFIRIDHEYLPNIALSNPVFIDCVNYIHDNI
                     DAHLSLKDIAMHCNISESYCSNLFVRYLSMNFKDYFTSIKLVNAINLLLSTKHSITTV
                     SELAGFNSHTNFANQFKNYLHFSPKQFRSLVSKITEPPQIHFQQDNVSQFTELISTID
                     LTAQLATNTTDIHIDDFNPKDRSQRAKVFVRFSNFNELFQFIFNEYYDINFEHLPKPV
                     VFIDDIHDIEISQTNYNLLNRCFEKLFEKNIGLAIAIKSTQQFETMKQLILTFLQGNQ
                     DYKTSKKLVKFMLVFCSNSMTAEEIHLCHLKIKNKNKEIKYSVTVDGFLETYSTVEQV
                     YDVMQRLKFHYYFIDIENSKTATHLITKNQHYHQTDTHFEQYKKFILDSGISSTQFVY
                     NNLSVSGFKYTNDGKNPIQLSDIVYHLIALLRYGGGISYQLLDDHSNYISLYNKYGSP
                     LPLMHLYKMFRPFVNEDIEITNNYVLSRKDNNYHFLLFNKINDRYMSDVKQDFIFHNE
                     LPQDSLMIIKTLNHEHGSIQHLLPISDQLVYIEKEILDELDKTNYPKTELAVQEETGR
                     TFELKLNHDEVKYICFKPS"
BASE COUNT         1896 a          798 c          903 g         2008 t
ORIGIN      
        1 aaaaatttgt tacaatctgg taaaaactct ttattaaata aattgattcg ttcatactga
       61 atagataagc aaaaaggagt tacataatga agaagaaaaa aaacgcgatt aaagttatca
      121 ttatattatt tatttcatta attgttgcag tagcattttt ctttggtctg aaaacatatc
      181 aaggccataa gaatattcaa ctaattgaca gttacttgga agagaaaaat ctaaaagata
      241 aaattaaatc agaaaaaaca gagtatagtg ctaaaaaagg tctcttctat aaggaagtta
      301 catttaaaga tgaacctggt gtaacttatg ttgtgcaacc aatcagtact aataaaggat
      361 tatttgttga aggctttgat acagagacta agaaaagttt gaaaactgca aagcataaat
      421 atttcaatca aaattataaa ccatcaaaat agtgaaacgt taaactggtg aagcagttta
      481 gtgccttcaa ttttaattat ttgaggtaaa cagtttttgt atggaaatta gtgatttttg
      541 acatctataa aagataaata ttttaatttt ttatagataa ttatcaattt tctgatataa
      601 ttgagtgatg tcagatgaag gggagtttgt actatggctc tattcaagag aaaaattagc
      661 ctacctatgc aagttgttat tgcattagtg ttaggtgttg tcgtaggact tttattatat
      721 ggacaagaaa acgtagcaaa ttacattaaa ccatttggtg acgtattttt aaatttaatt
      781 aaaatgatcg ttataccagt tgtattttgc tcactagcgc tttctatttc gaacgttggg
      841 gaatcgaaaa ctgtagggcg ttatggctgg aaaacaattt tatactttga aattattaca
      901 acaatcgcaa taggtttagg gattatcttc ggtaacctat ttaaaccagg tgctggatta
      961 gacccaacaa aattacctaa aggtgatatt tctaaatatc aatcaactgc acatgcagca
     1021 gaacaatcta catatggaaa tcattttatt gataccattg tacatattat tccgacaaac
     1081 ttttttgaag ctttaaataa gggtgaatta ttacctatta tcttcttcgc agtattcttt
     1141 ggattaggat tagctgctgt aggtaaaaaa gcagaaccag ttaaagaatt tttaagcgga
     1201 tcgcttgaag ctgtgttctg gatgattaat aaaattttaa aattagcacc acttggagtg
     1261 tttgcattca tttgtactac aattattaca tttggtgcat ccgcattatt accactatta
     1321 aaattagtat tagttgttgt ctttgcaatg gtgttctttg tattcgctat actaggacta
     1381 gttgcatgga tgtgtggtat taatatcatg aatattatta gaatcttgaa aagtgaattg
     1441 cttttagcat tttctacatc aagttcggaa gctgtacttc ctgtaatgat gaagaaaatg
     1501 gaaaacttcg gttctccaaa agaaattact tcttttgtta taccaattgg ttatacgttt
     1561 aacttagatg gatcagcact ttatcaatct attgcagcat tattcgttgc acagatgtat
     1621 ggaatgcact taacattatc agagcaaatt gtgttgatgt taacattaat gattacgtct
     1681 aaaggtatgg cggcagtacc aggaacttca atcgttgttt tattaacaac attaggtgcc
     1741 atgggcttac cggcacaagg tttagcatta attattggtg ttgaccgtat cttagatatg
     1801 gtacgtacat gtgtaaacgt tattggtaat gcattatcaa caatcgttat agctaaatgg
     1861 gaaaacgtat atgacaaagc aaaaggtcaa gaatatttaa aatcaattta aaaaatacta
     1921 tctgacattt aaagcccctt acaacctttg gttgttaggg gcttttatgc atgcgctaaa
     1981 gcgcaggcga acggaagcgt agtgacctca ggttgttagg gacttttatg catgcgctaa
     2041 agcgcaggcg aaccgaagcg tagtgacctc aggttgttag gggcttttat gcatgcgcta
     2101 aagcgcaggc gaaccggagc gcagtgacct caggttgtta ggggctttta tgcatgcgca
     2161 aagcgcaggc gaacggcaag tggagctatt caatatgaaa gtatttcaaa tataaaaagc
     2221 agcagtaaaa tgattccaaa ttggaaatca acttaccgct gcttttactc gtatatacct
     2281 tgatggcaac taaaatttgt aatttaaatt acaattagtg tttcatgtgc attttgccca
     2341 tgtcatgaat tttcatcatt aaaccatgtg gtacatcgtc atggtcaact tttttaactt
     2401 taacaatttt atctgaatct gctaatttat aaacaattac aaaatcacct tttttaactt
     2461 taacatctga atctgaaggt aatttaacat ttttttcaat actttcatct ttttcactaa
     2521 tcattttttc aatagtagta ttatctttca taattccata gtatgtatct ttctttccgc
     2581 cagtcatcat catgattact aatacaatac atacaattaa catgatggcg attaaactaa
     2641 taccaagacc aaaacctttt ttctttttca tggttcatca atctccttaa atgtagttaa
     2701 ttctgttata cctaaaagtg caacagtttg caaatgcagt atttatatta cataaaatta
     2761 atttaaaaag acacacccta atcacgttaa atgtgaataa agtgtgtcta aaaaacaata
     2821 cattgcaaat tgaattgtaa acatattaaa cactttaatt atagcgagtt attttaagta
     2881 tttaacattg ttatatctag aattcataaa agttatttcc tatatacact ttgtgttata
     2941 aatcgatttt gataggtgta tctaatgatt tatcattcat tagcttaatg gattgcggaa
     3001 ctactcttaa aacacataac tctggatctt cttttgaact gaaaaagctt ttgtcttgtg
     3061 tttcccatag ccaatcaatc acttcttgtt ctgtaacgat ttcgattgtc gcttccatct
     3121 caacgaaact acgattagtt gtgtcgttat agcctaacaa aacatatgct gctggattat
     3181 ctttaatttc tttgacctta gcagaatgga tattcgtttt agtatataaa gtaagaccat
     3241 cattataaaa gaccatatat ctactattag gtttattatt atatgcagtt gataatacac
     3301 caacttttga cgttgctaac acgttttcaa ttgcttgaat tgcttgtgag ttactcatta
     3361 caaatcatct ccttatgata taaatagcct ttaacgtaag aaactaaaca caagaggact
     3421 gttagtattt agcttggttt aaagcaaata tatttaacct cgtcgtgatt taacttgagt
     3481 tcaaatgtac gaccagtttc ttcttgaact gcaagctccg ttttagggta attcgttttg
     3541 tctaattcat ctaaaatttc tttttctata taaacaagtt gatcgcttat tggaagcaaa
     3601 tgttgaattg aaccatgttc atgattcaat gttttaataa tcatcaaaga gtcttgaggt
     3661 aattcattat ggaaaatgaa atcttgtttt acgtctgaca tatatcgatc attaatttta
     3721 ttgaataata agaaatggta gttattatct ttacgactca atacataatt atttgtaatt
     3781 tcaatatctt cattaacaaa aggtctaaac attttatata aatgcattaa tggtaggggg
     3841 ctaccatatt tgttgtacaa ggaaatataa tttgaatggt catctaataa ttgatagcta
     3901 atgccaccgc catatcgtaa taatgcgatt aagtgataca ctatgtcaga taattgaatt
     3961 ggattcttac catcattagt atatttaaat ccgcttacag acaggttatt atatacaaat
     4021 tgtgttgatg atatacctga atctaaaata aatttcttat actgttcaaa atgagtgtca
     4081 gtttgatgat agtgttggtt tttagtgata agatgtgttg cagtttttga attttcaata
     4141 tcaataaagt aataatggaa tttaagacgc tgcatgacat cataaacttg ttccacggtt
     4201 gaataagttt ctaaaaagcc atcgactgtc acactatatt taatttcttt atttttgttt
     4261 ttaattttaa gatgacataa atgaatttct tctgctgtca tagaattaga gcaaaatacg
     4321 agcataaatt taactaattt cttgcttgtc ttatagtctt gattgccttg taaaaatgtt
     4381 agtattaatt gcttcatcgt ttcgaattgt tgtgttgatt taatagcgat tgctaaacct
     4441 atattttttt cgaaaagctt ttcaaaacat ctatttaaca ggttgtaatt cgtttggcta
     4501 atctcaatat cgtgaatatc gtcaatgaaa acgacaggtt ttggtaagtg ctcaaagtta
     4561 atatcgtaat attcattgaa aataaattga aatagttcat tgaaattact aaatcgaaca
     4621 aatactttgg cacgctgact tcgatctttc ggattaaaat catctatatg aatatctgtt
     4681 gtatttgtag ccagttgagc tgttaaatca attgttgaaa ttaactcagt gaattgtgat
     4741 acgttatctt gttggaagtg tatttgtggt ggttcagtaa tcttggaaac gagtgaacgg
     4801 aactgtttag gactaaaatg caagtaattc ttaaattgat ttgcaaaatt tgtatgactg
     4861 ttaaaaccgg ccaattcaga aacagttgta atagaatgtt ttgtagaaag taataaattg
     4921 atggcgttaa caagtttaat acttgtaaaa taatctttaa aattcatact caaatatcga
     4981 acaaaaagat tagaacaata tgattcagag atgttgcaat gcatcgctat atcttttaaa
     5041 gacaagtgcg catcaatatt gtcatgaata tagtttacac agtctatgaa caccggattg
     5101 cttaaagcaa tatttggtaa atattcatga tctattctta taaaagcttc tttaagtaaa
     5161 gtgtctataa tactttgacc tatatttttc cccattaaag aagaacttgt tggtgtactg
     5221 attaaatcgg caattaatga cttaatataa ttacttgatt gtaataaatg acgatctaaa
     5281 taaccattaa agaaatcatc atcttgttga taaaaataaa aaacgggtat cattaattcg
     5341 attatatttt cgccgtggtt aatattataa atatcaccat ggttaattaa gataatataa
     5401 ttacttacgt ctttggtttc accattaatc gtaatattga gtttacttgt aagtgaaaat
     5461 agcaccataa tattattaat attacgactt ggtattgtcg ataatgtatg atgtatttta
     5521 agttggcatg tcatatttct ctctcctgcc taatatatat tgaaatctat catttaaatg
     5581 taaaacatgg aaatttttaa aatca
//
LOCUS       DALAGD010000131         5590 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.134, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000131 DALAGD010000000
VERSION     DALAGD010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.134"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(10..495)
                     /gene="tnpA"
                     /locus_tag="O2701_002508"
                     /pseudo
     CDS             complement(10..495)
                     /gene="tnpA"
                     /locus_tag="O2701_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500412.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(870..1616)
                     /locus_tag="O2701_002509"
     CDS             complement(870..1616)
                     /locus_tag="O2701_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HCZ4294375.1"
                     /translation="MPIEYFTHQFEEITYLSDDLQVKALMMTPHHEVNRIVVYLRGGK
                     GQVGRVRAGRLMQFSDSQTLVIGPYYRGNNGSEGKDEFYRGDLNDVTQLLRLLHDKYP
                     HAFIHMVGFSRGGLQGLLTFQDLPVTSYTIWGGVSDIDLMYEERVDLRGMLRRMIGHP
                     KKDRAAYEARQAIPNINENSPPILIVHGGKDQQVGIHHAYYLADQLELKGATHETFYQ
                     MAEGHVPRPPAMVETLTYIKEFMNQVESHS"
     gene            complement(1621..2094)
                     /gene="ytkD"
                     /locus_tag="O2701_002510"
     CDS             complement(1621..2094)
                     /gene="ytkD"
                     /locus_tag="O2701_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase YtkD"
                     /protein_id="HCZ4294376.1"
                     /translation="MKFRDKDNRQVNLTFKKDNEIADGNHVLAIPTFKNQLLFTKHNL
                     RGIEFPGGKRERGESSAEAVTRELYEETGAKVKNIYYIAQYTIETHDQTDFVKDVYFI
                     EVESLVSKNDYLETAGPVLFNCINDIELAQRSFLLQDSTILKCVERVQSLGFYQT"
     gene            2160..2417
                     /gene="yidD"
                     /locus_tag="O2701_002511"
     CDS             2160..2417
                     /gene="yidD"
                     /locus_tag="O2701_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCZ4294377.1"
                     /translation="MKKIFLAMIHFYQRFISPLTPPTCRFYPTCSEYTREAIQYHGAF
                     KGLYLGIRRILKCHPLHKGGFDPVPLKKDKSASKHSHKHNH"
     gene            complement(2414..3415)
                     /gene="menC"
                     /locus_tag="O2701_002512"
     CDS             complement(2414..3415)
                     /gene="menC"
                     /locus_tag="O2701_002512"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCZ4294378.1"
                     /translation="MKLTALHFYKYSEPFKSQIVTPKVTLTHRDCLFIELIDDKGNAY
                     FGECNAFQTDWYDHETIASVKHVIEQWFEDNRNKSFETYEAALKLVDSLENTPAARAT
                     IVMALYQMFHVLPSFSVAYGATASGLSNKQLESLKATKPTRIKLKWTPQIMHQIRVLR
                     ELDFHFQLVIDANESLDRQDFTQLQLLAREQVLYIEEPFKDISMLDEVADGTIPPIAL
                     DEKATSLLDIINLIELYNVKVVVLKPFRLGGIDKVQTAIDTLKSHGAKVVIGGMYEYG
                     LSRYFTAMLARKGDYPGDVTPAGYYFEQDVVAHSGILKEGRLEFRPPLVDITQLQPY"
     gene            complement(3420..4898)
                     /gene="menE"
                     /locus_tag="O2701_002513"
     CDS             complement(3420..4898)
                     /gene="menE"
                     /locus_tag="O2701_002513"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCZ4294379.1"
                     /translation="MDFWLYKQAQQNGHHIAITDGQESYTYQNLYCEASLLAKRLKAY
                     QQSRVGLYIDNSIQSIILIHACWLANIEIAMINTRLTPNEMTNQMKSIDVQLIFCTLP
                     LELRGFQIVSLDDIEFAGRDITTNSLLDNTMGIQYETSNETVVPKESPSNILNTSFNL
                     DDIASIMFTSGTTGPQKAVPQTFRNHYASAIGCKESLGFDRDTNWLSVLPIYHISGLS
                     VLLRAVIEGFTVRIVDKFNAEQILTMIKNERITHISLVPQTLNWLMQQGLHEPYNLQK
                     ILLGGAKLSATMIETALQYNLPIYNSFGMTETCSQFLTATPEMLHARPDTVGMPSANV
                     DVKIKNPNKEGHGELMIKGANVMNVYLYPTDLTGTFENGYFNTGDIAEIDHEGYVMIY
                     DRRKDLIISGGENIYPYQIETVAKQFPGISDAVCVGHPDDTWGQVPKLYFVSESDISK
                     AQLIAYLSQHLAKYKVPKHFEKVDTLPYTSTGKLQRNKLYRG"
     gene            complement(5057..5512)
                     /locus_tag="O2701_002514"
     CDS             complement(5057..5512)
                     /locus_tag="O2701_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4909 domain-containing protein"
                     /protein_id="HCZ4294380.1"
                     /translation="MKICLMLYLLIFLSSCSTNNKIVGQRFEIQQHNETIGSIYFSAD
                     YAHIRGIEKGTAKYFIDKVGSKRYLFIEYIPDNVLNCKPDFWKTLKYKKDKVTYYVYL
                     IENLDDEVFHLSALQEMNRIPIDIADDVATMGKSPHQNDRMTLKLNKNN"
BASE COUNT         1661 a         1128 c          829 g         1972 t
ORIGIN      
        1 cctaaagggt tactcttttt tctttctttt tttatttttc tctccagtga aaggatctaa
       61 atattcttcc attgagattt ggtctgcaac gatatcctct tgtaattgat tacgaatata
      121 attttcaatc acttttttat ttctacctac tgtatccaca taaaatcctt tacaccaaaa
      181 ctttctattt ccatatctat actttaagtt agcatgtcta tcaaatatca ttaaactact
      241 ttttcctttt aaatagccaa caaatgatga taccccaagt ttgggtggta tactaactaa
      301 catatggata tgatctttac atgcctctgc ttcaattatc tctacacctt ttctttcaca
      361 taattgacgc aatataatcc ctatatcttt ttttattttt ccatatatca cttgtcttct
      421 gtatttaggt gcaaagacaa tatgttactt acaattccat tttgtatgtg ctaaactgtt
      481 tgtgtcagat gacattaaat agcatctcct cgtgttgatt attttggttg gctgaccaat
      541 atttattcta gcacgtagag atgcattttt tgtgacaatg gtagaacctt ttctgaacca
      601 tacgcatagc gtatggtttt ctttttacaa ttaaagagcc aaccgttgtt atagtctaac
      661 aatggttggc tcctcttatt ttatgtgcta aaaatttata ggcaatttta ttacaacaat
      721 gtacatttaa ggtgaccttc atgccaaaat cgcatcactc atttaatgga agcagcacgt
      781 cttcatataa agtaccgatc cctaattcaa cgcatgtagt accacatctt caaagcttga
      841 tagttcccat gcgcacacca cgtttcatac tagctatgcg actcaacttg gttcataaac
      901 tctttaatat aagtcaatgt ttcaaccatc gctggtggtc ttggcacatg tccttctgcc
      961 atttgataaa atgtttcatg cgtggcacct tttaactcta gttggtccgc taaataatac
     1021 gcatgatgaa taccaacttg ctggtctttc cctccatgta caattaatat tggcggactg
     1081 ttttcattaa tgtttggaat cgcttggcgt gcctcatatg ccgctcgatc ttttttcgga
     1141 tgaccaatca ttcttcgtag catgcctctt aaatcgacac gttcttcata cattaaatca
     1201 atatctgaga caccacccca gattgtataa cttgttactg gtaagtcttg aaatgtcaac
     1261 aatccttgta aaccacctcg cgaaaaacca accatgtgga taaatgcatg tggatattta
     1321 tcatgtagca accttaataa ttgcgtcaca tcatttaaat cgccacggta aaattcgtct
     1381 ttgccttcac tcccattgtt acctcggtag tatggcccaa tcactaaagt ttgactatct
     1441 gaaaattgca ttaatctacc tgcgcgcaca cgtcctactt gacctttgcc acctcgcaaa
     1501 taaactacaa tgcgatttac ttcatgatgt ggtgtcatca ttaaagcttt tacttgtaag
     1561 tcatctgaca aatatgtaat ttcttcgaat tgatgcgtaa aatattcaat tggcattcgt
     1621 ttacgtttga taaaacccaa gtgattgcac cctctctacg cattttaaaa tggtactatc
     1681 ttgcagtaag aaactccgtt gtgcgagttc aatatcattg atacagttaa acaacactgg
     1741 ccctgctgtt tctaaataat cgttcttgct taccaatgat tcaacttcga taaaatatac
     1801 atcttttaca aaatcagttt gatcatgtgt ttcaatggta tattgtgcta tgtaataaat
     1861 atttttaact ttggcgcctg tttcttcata taattcacgt gtaactgctt cagcactact
     1921 ttccccgcgt tcccttttac caccaggaaa ttcaatcccc cgtaaattat gtttggtaaa
     1981 aagcaattga tttttaaacg ttggaatagc tagcacatga ttgccatctg ctatctcatt
     2041 atccttttta aatgtcaaat taacttgacg attatcttta tccctaaact tcacgcgcat
     2101 cacatcccta cattgtatgt taatataata gttaattact atcgttggag gcattaatta
     2161 tgaaaaagat attcttggcg atgattcatt tttatcaacg tttcatttcg ccactcactc
     2221 caccaacttg tcgtttttat ccaacatgtt cagagtacac tagagaagcg attcaatacc
     2281 acggtgcttt caaaggcctt tatttaggta tccgtcgtat tttaaaatgt catccgcttc
     2341 ataaaggcgg ctttgaccct gttccgttaa aaaaagacaa gtcagcaagc aagcattcac
     2401 ataaacataa ccattaatat ggttgtaatt gagttatatc cactaaaggg gggcgaaatt
     2461 cgagtcgccc ctcttttaat atgcctgaat gcgccaccac atcttgttca aaataataac
     2521 ctgctggtgt aacatctcct ggataatcac ctttacgagc aagcatcgct gtaaaatagc
     2581 ggcttaaacc atattcgtac atgccgccaa taaccacttt tgcaccatga cttttcaaag
     2641 tatcaattgc cgtttgcact ttatcaatgc cacctagacg aaatggtttt aatacaacaa
     2701 ctttcacatt gtataattct atcaaattaa ttatgtccaa caacgatgtt gccttttcat
     2761 caagggctat tggaggtatt gttccatccg ctacttcatc aagcatggag atatctttaa
     2821 atggctcttc gatataaaga acctgttcac gcgctaataa ctgtaactgt gtgaaatctt
     2881 gacgatccaa ggactcattt gcatctataa ccaattgaaa gtgaaagtct aattcccgta
     2941 acactctaat ttgatgcatg atttgaggcg tccattttaa tttaattctg gtcggctttg
     3001 ttgcttttaa tgactctagt tgtttatttg ataagccgct cgctgtcgct ccatatgcta
     3061 ctgaaaatga aggcagtaca tgaaacattt gatacaatgc catgacaata gttgcccttg
     3121 cagcaggcgt attttccaat gaatctacta attttagtgc tgcttcatac gtttcaaatg
     3181 atttatttct attatcttcg aaccattgct caattacatg tttcactgag gcaattgttt
     3241 catgatcata ccaatctgtt tgaaaagcgt tacattcccc gaaatatgca tttcctttgt
     3301 catcaatcaa ttcgataaac aaacaatcac gatgcgttaa agtgactttc ggtgttacaa
     3361 tttgtgactt aaatggctca ctatatttat aaaaatgcaa agctgtcaac ttcatcaaat
     3421 catcctctat acaacttatt tctttgtaat ttacctgttg atgtataagg taaagtatca
     3481 accttttcaa agtgtttcgg tactttatat ttcgctaaat gttgtgataa atatgcaatc
     3541 aattgtgcct ttgaaatgtc actttcactg acaaaatata atttaggcac ttggccccaa
     3601 gtatcatcag gatgccctac acatactgcg tcactgatac ctggaaattg cttcgctacc
     3661 gtttcaattt gatatggata aatattttca ccgccactaa taattaaatc tttacgtcgg
     3721 tcataaatca tgacataacc ttcatgatct atttcagcaa tgtcacccgt attaaaataa
     3781 ccattttcaa acgtacccgt taaatctgtt ggatacaaat atacattcat cacattggcg
     3841 cctttaatca ttaattctcc atgaccttct ttattaggat ttttaatttt tacgtcaaca
     3901 ttggcacttg gcatccctac agtgtcagga cgtgcatgca acatttccgg tgttgctgtt
     3961 aaaaattgcg aacatgtctc agtcatacca aatgaattat aaattggcag gttatattgt
     4021 aatgccgtct ctatcatagt ggcagataat ttagcaccgc cgagtaatat tttttgtaaa
     4081 ttataaggtt catgtaaacc ttgttgcata agccaattta aagtttgtgg cacaagcgaa
     4141 atgtgcgtga ttcgttcatt tttaatcatc gttaaaattt gttcggcatt gaatttatca
     4201 acaatgcgca cagtaaaacc ttcaataaca gctcttaaaa gtacactgag acccgaaata
     4261 tgataaatcg gcaagacaga tagccaatta gtgtcacgat caaatcccaa gctctcttta
     4321 catccgattg cactggcata atgattacga aacgtttgtg gcaccgcttt ttgagggccc
     4381 gttgtccctg atgtaaacat aatcgatgca atgtcatcta aattaaatga tgtatttaat
     4441 atgttggacg gcgactcttt cggcaccaca gtttcattcg atgtttcata ttggataccc
     4501 attgtgttgt ccaacaaact gttcgttgta atatcccttc cagcgaattc aatatcatcc
     4561 agcgatacaa tttgaaaccc tcgtaattcc agtggcaagg tacaaaaaat caattgtaca
     4621 tcgattgact tcatctgatt cgtcatctca ttaggtgtca accttgtatt aatcatcgca
     4681 atttcaatat ttgccaacca acatgcatgt attaaaatga tcgattgaat cgaattatct
     4741 atgtatagcc caacacgaga ttgttgataa gccttgagtc ttttagccaa tagactcgct
     4801 tcacagtata aattttgata agtataagat tcttgaccgt ctgttatcgc aatatgatgt
     4861 ccattttgtt gtgcttgttt atataaccaa aagtccatgc gttattcctc caaaatcatt
     4921 tacattataa ttataacgat tttatgacat tctagcagtg gttatgttta aaaatataaa
     4981 aaagtagacg aattgatgca ttgatatgat tgttataatg ctcaatacat atcgttatat
     5041 cattcgtcta ctattatcag ttatttttat ttaattttag tgtcattctg tcattttgat
     5101 gtggtgattt acccattgtt gccacatcat ctgcaatgtc aattggtata cggttcattt
     5161 cttgtaatgc acttaaatgg aatacttcat catctaaatt ttcaatgaga tatacataat
     5221 atgttacctt gtccttttta tattttaacg ttttccaaaa gtccggcttg caattcaata
     5281 cattatccgg aatatattca ataaataagt aacgtttgct gcctactttg tctatgaaat
     5341 attttgcagt gcctttttct atacctctta tatgtgcata gtctgctgaa aagtaaatac
     5401 tacctattgt ttcattatgt tgttgtattt caaatcgttg gcctactatt ttattatttg
     5461 tgctacaact acttaaaaaa atcagtagat atagcattaa acatattttc atccccttga
     5521 attttaaaaa ctttttcaaa agcaacacct ctaaaaataa atacaatata ttatagcaaa
     5581 tagaaattat
//
LOCUS       DALAGD010000132         5472 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.135, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000132 DALAGD010000000
VERSION     DALAGD010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5472
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.135"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            113..712
                     /locus_tag="O2701_002515"
     CDS             113..712
                     /locus_tag="O2701_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCZ4294381.1"
                     /translation="MAFKLPNLPYAYDALEPYIDQRTMEFHHDKHHNTYVTKLNATVE
                     GTELEHQSLADMIANLDKVPEAMRMSVRNNGGGHFNHSLFWEILSPNSEEKGGVIDDI
                     KAQWGTLDEFKNEFANKATTLFGSGWTWLVVNDGKLEIVTTPNQDNPLTEGKTPILLF
                     DVWEHAYYLKYQNKRPDYMTAFWNIVNWKKVDELYQAAK"
     gene            1080..1805
                     /gene="sasD"
                     /locus_tag="O2701_002516"
     CDS             1080..1805
                     /gene="sasD"
                     /locus_tag="O2701_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070002951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasD"
                     /protein_id="HCZ4294382.1"
                     /translation="MKKLATVGSLIVTSTLVFSSMPFQNAHADTTSMNVSNKQSQNVQ
                     NHRPYGGVVPQGMTQAQYTELEKALPQLSAGSNMQDYNMKLYDATQNIADKYNVIITT
                     NVGVFKPHAVRDMNGHALPLTKDGNFYQTNVDANGVNHGGSEMVQNKTGHMSQQGHMN
                     QNTHMNQQPHMQQGHMQSSNHQMMSPKANMHSSNHQMNQSNKKVLPAAGESMTSSILT
                     ASIAALLLVSGLFLAFRRRSTNK"
     gene            complement(1997..2752)
                     /locus_tag="O2701_002517"
     CDS             complement(1997..2752)
                     /locus_tag="O2701_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCZ4294383.1"
                     /translation="MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCAR
                     FDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLARV
                     SMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNHSKQADAFCFTFIPLNVID
                     TFKVNTQNEDDMKTFVEQTIYSNAYQSDLKMSITKAPHFKNHSHVFDGDTHCWLIIET
                     LYAQTPYPIMINKWYIPQEISELTLTRIRQSDY"
     gene            2988..3695
                     /gene="deoD"
                     /locus_tag="O2701_002518"
     CDS             2988..3695
                     /gene="deoD"
                     /locus_tag="O2701_002518"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCZ4294384.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            3702..5054
                     /gene="tet(38)"
                     /locus_tag="O2701_002519"
     CDS             3702..5054
                     /gene="tet(38)"
                     /locus_tag="O2701_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HCZ4294385.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFPLL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVSLNRWTQSEK
                     "
     gene            5135..>5472
                     /gene="deoC"
                     /locus_tag="O2701_002520"
     CDS             5135..>5472
                     /gene="deoC"
                     /locus_tag="O2701_002520"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCZ4294386.1"
                     /translation="MKFEKYIDHTLLKPESTRTQIDQIIDEAKAYNFKSVCVNPTHVK
                     YAAERLADSEVLVCTVIGFPLGASTTATKAFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQDIEAVV"
BASE COUNT         1883 a          838 c          974 g         1777 t
ORIGIN      
        1 tcgaatattt aaattttata taattggata taacaaataa ataataatta ttgcaaaaca
       61 cacccgaaat taattattat aaaagtatat tcataaaagg aggaatatac ttatggcatt
      121 taaattacca aatttaccat atgcatatga tgcattggaa ccatatatag atcaaagaac
      181 aatggagttt catcacgaca aacatcacaa tacgtacgtg acgaaattaa acgcaacagt
      241 tgaaggaaca gagttagagc atcaatcact agcggatatg attgctaact tagacaaggt
      301 accggaagcg atgaggatgt cagtccgtaa taatggcggt ggtcatttta accattcatt
      361 attctgggaa atactatcac ctaattctga agaaaaaggt ggcgtaatag atgacatcaa
      421 agcgcagtgg ggcactttag atgaatttaa aaatgaattt gcaaataaag caacaacatt
      481 atttggatca ggttggactt ggttagttgt taatgatggc aaattagaaa ttgtgacaac
      541 gccaaaccaa gataatccat taacagaagg caaaacacca atcttactat ttgatgtttg
      601 ggagcatgcc tactatctga aatatcaaaa taaacgtcca gactatatga ctgcattttg
      661 gaacattgtt aactggaaaa aagttgatga attataccaa gcagcaaaat aatataactt
      721 aatataattg aggtggagca tctacaaggt gttccacttt tttgtactta atatctttac
      781 ttatttgttt ttgtgtaatg ggatagcacg taaaagtgga aaaataataa gataaatttc
      841 aactcgaaat accttattat tgttgactat aacgatgacc taaatattat atacattaga
      901 caacactatt aattaatcaa catattgaca ttaaataaat tgacaaaata agtaattatt
      961 gtgaatctaa agtgaaattt ttataaaaaa atgtaatgat tcaaaaattt tgttgcattt
     1021 cttttgtaat cgtatgataa tgtaaatgta atcaaattgt aatataaggg gacaagacaa
     1081 tgaaaaaatt agcaacagta ggttctttaa ttgtaacaag cactttagta ttctcaagta
     1141 tgccttttca aaatgcgcat gccgacacaa cttcaatgaa tgtgtcgaat aaacaaagcc
     1201 aaaatgtaca aaatcatcgt ccttatggcg gagtagtacc acaaggaatg acgcaagcac
     1261 aatatactga attagagaaa gctttacccc aattaagcgc tggcagtaat atgcaagact
     1321 ataatatgaa attgtatgat gcgacgcaaa atattgctga taaatacaat gtgataatta
     1381 caactaatgt aggggtattt aaaccacatg ctgttagaga tatgaatggc catgcgttac
     1441 ctttaacaaa agatggcaat ttttatcaaa cgaatgtaga tgcaaatggt gttaatcatg
     1501 gtggtagtga aatggtgcaa aataaaacag gtcatatgag tcaacaaggc catatgaatc
     1561 agaacacaca catgaaccaa cagccacaca tgcaacaagg tcatatgcaa tcatcaaacc
     1621 atcaaatgat gagtccaaaa gcaaatatgc attcatcaaa tcatcaaatg aaccaaagta
     1681 acaaaaaagt tttaccagct gctggtgaaa gtatgacatc aagtattctt actgcaagta
     1741 ttgccgcact actattagta tctgggttat tcttagcatt tagacgacgt tcaacaaata
     1801 aataaacata atacgattaa taatagaaaa atcgtgtgat tatctgagcg agcctaggac
     1861 ataaatcaat gtcctaggct cgctaatgtt atattggcag tagttgactg aatgaaattg
     1921 cgcttgtaac aagcttttcc atttcttacc aactccctaa atagtcatca aaaaatttct
     1981 tatattttaa taatttttaa taatccgatt gtcttatacg tgtcagtgtt aattcagata
     2041 tttcctgtgg aatataccac ttattaatca taattggata aggtgtttgt gcgtacagtg
     2101 tttcaataat cagccaacaa tgtgtatcac catcaaacac gtgactatga tttttgaagt
     2161 ggggcgcttt ggtaatagac atttttaaat ctgattgata tgcattgcta taaatcgttt
     2221 gctcaacgaa tgtcttcatg tcgtcttcgt tttgtgtatt cactttaaat gtgtcaatga
     2281 catttaacgg tataaaggta aagcaaaatg catcagcttg cttagaatga ttgtcctttt
     2341 tttgataata gcgttccatt gcaatgacgg cagaaggatg gtttgcaaac aaatgatttg
     2401 tatattcact ttctaaatca acacgataat taattgatga catagatacg cgagctagca
     2461 atatttgatc aagtggatgc ttaaattgat ccatacttga agcgtgttgg gcatttgttt
     2521 gtggaataac aaagtgtccc ttccctcttg tactctctac gatgccatct tcggctaaca
     2581 attttatagc ttggcgcaaa gtcatacgac tgacatcaaa gcgcgcacaa agttcctttt
     2641 cagtaggtaa tgcatggcca ctcggatatt ttcctatttg aatttcttta tataacgtat
     2701 tataaatcgt taaaaatttt ggttgtgttt gcgtcacgta gacaacctcc ataaagttac
     2761 ttaatcactc tcatcataca ataattttta ctcaaattgg aaaaattata aaaattaaat
     2821 atagataggc tttgaaaatt agttttatac aaggttagta gctgtaactg taaaatgttc
     2881 ttaatattgt caaaatgtaa tgcttgaaag cgcttttaaa aaatattatt atatacatgg
     2941 ttagacaaat agacaaatca ctatacaaat attgggagga atattttatg aaatcaacac
     3001 cacacattaa accaatgaat gacgtcgaaa ttgcagaaac ggttctattg ccaggagatc
     3061 cgttaagagc taagttcatt gcagaaactt atttggatga tgtggaacag ttcaatacag
     3121 tgcgaaacat gtttggtttt accggaacat ataaaggtaa aaaagtttct gtcatgggtt
     3181 caggtatggg tatgccatct attggcattt actcttatga attaattcat acatttggtt
     3241 gtaaaaaatt aattcgcgtt ggctcttgtg gcgcgatgca agaaaacatt gatttatatg
     3301 atgtgattat tgcacaaggt gcctctactg attcaaatta cgttcaacaa tatcaattac
     3361 caggtcattt tgcgccaatt gcttcttatc aattattaga aaaagcagtt gaaacagcac
     3421 gtgacaaagg tgtacgtcat catgtaggta atgtgttatc aagtgatatt ttctataacg
     3481 cggatacaac agcgagtgaa cgttggatgc gtatgggtat tttaggtgta gaaatggaat
     3541 cagctgcatt atacatgaat gcaatttacg ctggtgtcga agcattaggt gtgttcacag
     3601 tgagcgatca tttaattcat gaaacgtcaa caacacctga ggaaagggaa cgtgcattta
     3661 cagatatgat tgaaattgca ctgtcattgg tgtagatgat tatgaatgtt gaatattcta
     3721 aaataaagaa agcagtacct attttattat tcttatttgt attcagtttg gttatagaca
     3781 actcatttaa attgatttct gtagccattg ctgatgactt aaacatatct gtaacgacag
     3841 taagttggca agcgacatta gccggtttag taattggtat tggcgctgta gtatacgctt
     3901 cattatctga tgccattagt atacgcacac tatttattta tggcgtgata ttaatcatta
     3961 tcggatcaat tattggttac attttccaac atcaattccc attactttta gttggacgta
     4021 ttattcaaac tgccggttta gctgctgcag agacattata tgtgatatat gttgcaaagt
     4081 atctttctaa agaggaccag aagacttacc ttggcttaag tacgagcagt tattccttgt
     4141 cattagttat cggtacatta tcaggtggat ttatttctac gtatttacac tggacaaata
     4201 tgtttttaat tgcattaatc gtagtattta cgttgccatt cctatttaaa ttattaccaa
     4261 aagaaaataa tacgaataaa gctcatttag attttgttgg cttaattcta gtggcaacta
     4321 ttgctacaac agtcatgctg tttattacga actttaattg gttatatatg attggtgcct
     4381 taattgcgat tatcgttttt gcgctatata ttaaaaatgc gcaacgtcca ttagtaaata
     4441 aatcattttt ccaaaataaa cgttatgctt catttttatt tatagtattt gtaatgtatg
     4501 ctatccaatt gggttatatt tttacgttcc cattcataat ggagcaaatt tatcatctgc
     4561 aactagacac aacatcactg ttattagtac cgggttatat agtagcagtc attgttggtg
     4621 cactaagtgg taaaatcggc gaatatctga attcaaaaca agcgattatc acagcaatta
     4681 ttttaatagc actgagcttg attttacctg catttgcagt aggtaatcac atttcaatct
     4741 tcgtcatttc tatgatattc tttgcaggta gctttgcttt aatgtatgca cctttactta
     4801 acgaagccat taaaacaata gatcttaata tgacaggtgt ggctattggt ttttataatt
     4861 taattattaa tgtggcggta tctgtaggta ttgcgattgc tgcggctcta atcgatttta
     4921 aagcattaaa tttcccaggc aatgatgcat taagttcaca tttcggtatt attttaatta
     4981 ttttaggttt aatgagtatt gtcggattag ttttattcgt cagcttaaat cgttggacac
     5041 aatctgaaaa ataaatagat ataaattcgc gagatatatt cgtatttata gtaaaattaa
     5101 ataaagagat tatataacac gaggagtagt aagtatgaaa tttgagaaat atatagatca
     5161 cactttattg aagcctgagt caacacgtac gcaaatcgat caaatcatcg atgaagcgaa
     5221 agcatacaat tttaaatctg tatgtgtgaa tccaacacat gttaaatatg cagcagagcg
     5281 actagctgat tcagaggtgc tcgtttgtac ggtaatagga ttcccattag gtgcgtcgac
     5341 aactgcaacg aaagcatttg aaacagaaga tgcaattcaa aatggtgcag atgaaattga
     5401 catggtcatc aacatcggcg cattaaaaga tggacgtttt gatgatgtac aacaagacat
     5461 tgaagcagtg gt
//
LOCUS       DALAGD010000133         5425 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.136, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000133 DALAGD010000000
VERSION     DALAGD010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5425
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.136"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(<1..487)
                     /locus_tag="O2701_002521"
     CDS             complement(<1..487)
                     /locus_tag="O2701_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4294387.1"
                     /translation="MIEIKTLTNNDFNEYKRLVSTVNEEFTQDSHYSQTMTDTLIHDI
                     LNQGSPKCIVFGCYENETLIATAALEQIRYVGKEHKSLIKYNFVTNNDKSINSELINF
                     IINYARQNNYESLLTSIVSNNIGAKVFYSALGFDILGFEKNAIKIGNTYFDEHWLFYD
                     LI"
     gene            complement(886..1308)
                     /locus_tag="O2701_002522"
     CDS             complement(886..1308)
                     /locus_tag="O2701_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCZ4294388.1"
                     /translation="MAIHYETKATNVGGRKGHVYTDDRALDIDIVPPAQADGKATNPE
                     QLFAAGYASCFNGAFDLILKQNKVRDAHPEVTLTVRLEDDSDSESPKLSVSIDATIKN
                     VISQEEAEKYLQMAHEFCPYSKATQGNINVDLNVNVVD"
     gene            1456..2172
                     /locus_tag="O2701_002523"
     CDS             1456..2172
                     /locus_tag="O2701_002523"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCZ4294389.1"
                     /translation="MTHVEVVATIAPQLSIEETLIQKINHRIDAIDVLELRIDQIENV
                     TVDQVAEMITKLKVMQDSFKLLVTYRTKLQGGYGQFTNDSYLNLISDLANINGIDMID
                     IEWQADIDIEKHQRIITHLQQYNKEVVISHHNFESTPPLDELQFIFFKMQKFNPEYVK
                     LAVMPHNKNDVLNLLQAMSTFSDTMDCKVVGISMSKLGLISRTAQGVFGGALTYGCIG
                     VPQAPGQIDVTDLKAQVTLY"
     gene            2255..2794
                     /locus_tag="O2701_002524"
     CDS             2255..2794
                     /locus_tag="O2701_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HCZ4294390.1"
                     /translation="MELQQAIANRRSVKKFKRDMHIDDALLYQAIEKAADAPNHGMRE
                     PWRVVHVPKDRLGDMSKDISKFAFPNELDKQQCHYDAVTKLGGMLLLILKTDPRQRQN
                     DENYFAFGAYAQNLMLLLYEAGIGTCWKSPLYIYDPKVRKTLGIKKDEVLAGFLYLTD
                     LEGDMPKAPRKNRNLITLY"
     gene            complement(2944..3264)
                     /locus_tag="O2701_002525"
     CDS             complement(2944..3264)
                     /locus_tag="O2701_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCZ4294391.1"
                     /translation="MQSIKSNESFKSVINSDTPVIVKFEAGWCPDCRAMDLWIDPIVE
                     QYNDYQWYTVNRDELEDVVVENEVMGIPSLLVFKNGDKIAHLHSANAKSPEQVESFLA
                     ETFK"
     gene            3408..3764
                     /locus_tag="O2701_002526"
     CDS             3408..3764
                     /locus_tag="O2701_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HCZ4294392.1"
                     /translation="MIKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKT
                     IIANTGVEINKLFNTHGAKYRELDLKNKLQTLSDDEKLELLSSDGMLVKRPLAVMGDK
                     ITLGFKEDQYKETWLA"
     gene            3922..4302
                     /gene="gcvH"
                     /locus_tag="O2701_002527"
     CDS             3922..4302
                     /gene="gcvH"
                     /locus_tag="O2701_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCZ4294393.1"
                     /translation="MAVPNELKYSKEHEWVKVEGNVAIIGITEYAQSELGDIVFVELP
                     ETDDEINEGDTFGSVESVKTVSELYAPISGKVVEVNEELEDSPEFVNESPYEKAWMVK
                     VEISDESQIEALLTAEKYSEMIGE"
     gene            4481..5359
                     /locus_tag="O2701_002528"
     CDS             4481..5359
                     /locus_tag="O2701_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HCZ4294394.1"
                     /translation="MLNIQDVSHLSKKEQKAYNRFVESVENGNLPVLPCIEMDLKEMQ
                     EETLNQSKIGGMPFLKYFKDIPLDENNVPMVLLAQINLDDLPEQQELFPVKEGILQFW
                     ISSEDQMYGMSENLKGNNINSRLVYIKEPITDLSLENIQAHLKSLDADNEDIPFSGAF
                     SIEFRLSKQTITCTDYKYDEDVLALWNKVNPSFALKSMFGGYDELMEPVCNTFTAKEP
                     FNQLGGYPYFDQIDPRTNDQELKMYDRVLLQIDSTRDGNSSIIWGDLGIANILVKSTD
                     LEAMKFDDYMYSWDCS"
BASE COUNT         1941 a          738 c          952 g         1794 t
ORIGIN      
        1 taatcaaatc ataaaaaagc caatgttcat cgaaataggt atttccgatt ttaattgcat
       61 ttttctcaaa accaagaatg tcgaatccta gtgcactata gaaaacttta gcacctatgt
      121 tgtttgacac aattgatgta agtaaagatt cgtaattgtt ctgccgtgca taattaataa
      181 tgaaattaat gagctcgcta ttaatcgatt tatcgttatt agtaacaaag ttgtatttaa
      241 ttaatgattt atgttctttt ccaacgtatc gaatttgttc taaggcagct gttgcgataa
      301 gtgtttcgtt ttcataacag ccaaatacaa tacatttcgg tgaaccttga tttaaaatgt
      361 catgtattaa ggtgtcagtc attgtttgac tataatgtga atcttgagtg aattcttcat
      421 tgactgtcga aacaagtctc ttatactcat taaaatcatt attcgttaat gttttaattt
      481 caatcattac tgacctcctt aatcattatt aagaaagatt ttgtactaaa aaaatttctt
      541 aattacatat taaaatgttt attaaaattt tgcaatcact gatttatatg cacattcttg
      601 taaatggatt atcaatttaa ttatatttat ttttaagttc aggaaattat ggaattggtt
      661 attggtcgac actggacttt tatttgtaga cggtatgcga agaagtcaag ccagtattgg
      721 aaccggtcac atttatgatg actaaggtac aatcattttc agttaaatat tgccagaaat
      781 ttcaatcacg aatagaaaaa ataaccaacc aaaaattttt ggttggcaaa tttgaatttt
      841 atgtgaattt tattaataac gttgaataat ctctttaagt taatgctaat ctacaacatt
      901 tacatttaaa tcgacattaa tatttccttg agtcgctttt gaatatggac aaaattcatg
      961 agccatttgt aaatattttt cagcttcttc ttgagatata acatttttaa ttgtcgcatc
     1021 aattgaaaca cttaatttag gactttctga gtctgaatca tcttctagtc tcactgttag
     1081 tgttacttct ggatgagcat cacgcacttt gttttgcttt aaaattaggt cgaaagcacc
     1141 gttgaagcaa gatgcataac ctgctgcaaa taattgttcg gggttagtag ctttaccatc
     1201 tgcttgagca ggcggaacga tatcaatatc taatgctcga tcatcagtat aaacatgtcc
     1261 tttacgtccg ccaacattcg tcgctttagt ttcataatgt attgccatac ttataacctc
     1321 ctaatttaaa atacatttat gctttaccca ttcgaaaata ccttaatcat tttcatttat
     1381 agcattgttt gattgaagga taaaaagttg ttgtttacaa taaaaataat gagtatctga
     1441 aatgagggat tcactatgac acatgtggaa gtagtagcga ctatcgcgcc acaattatct
     1501 atcgaagaaa ctttaattca aaaaattaat catcgtattg atgcaataga cgtattagaa
     1561 ttacgaattg atcaaattga aaatgtcaca gttgatcaag tggcagaaat gattacaaag
     1621 ctgaaggtta tgcaagattc attcaaatta ttagttacgt atcgtacaaa gttacaaggt
     1681 ggctatgggc aatttacaaa tgactcgtat cttaatttaa tatcagactt agcaaatatc
     1741 aatggcatag atatgattga tatagaatgg caagcagata ttgacattga aaaacatcaa
     1801 cgaatcatta cacatttgca acagtataat aaagaggtgg ttatatcaca tcataatttc
     1861 gaaagtacgc ctccattaga tgaattgcaa tttatatttt ttaaaatgca aaaattcaac
     1921 ccagaatacg ttaaattagc agtaatgcca cataataaaa atgatgtgtt aaatttattg
     1981 caggcaatgt ctacattttc agatactatg gactgcaaag ttgttggtat ttcaatgtct
     2041 aaacttggac taataagtag aacggctcaa ggcgtttttg gtggtgcgtt gacttatggt
     2101 tgtatcggag taccacaagc tccaggacag attgatgtta ctgatttaaa agcacaagtg
     2161 actttatact aataaaatga attaggttgc ttttgtgtaa gtgctgtctt ataattgaga
     2221 agtattatca ttgttcgtgg aggagcgatt ttagatggaa ttacaacaag caatagctaa
     2281 tagaagaagt gtgaaaaaat ttaaaagaga tatgcacata gatgacgcat tgctatatca
     2341 agcaattgag aaagctgctg atgctccaaa tcacggaatg agggaaccat ggagagttgt
     2401 gcatgttccg aaagacagat taggagatat gagtaaggat atttctaaat ttgcatttcc
     2461 taatgaatta gataagcaac aatgtcatta tgatgcagtt acgaaactag gtggcatgtt
     2521 attgcttatt ttaaaaacag atccaagaca acgtcaaaat gatgaaaact actttgcatt
     2581 tggtgcatat gcacaaaatc ttatgttgtt actttatgaa gcgggaatag gtacatgttg
     2641 gaaatcgcca ttatatatct atgatcctaa agtaagaaaa acacttggta taaagaaaga
     2701 tgaagttctt gctggattct tatatttaac ggatttagaa ggagatatgc ctaaagcacc
     2761 acgtaaaaat agaaacttaa ttacattata ttaatatgta taattataga aacattaata
     2821 aaagctgagt catgaattga tggacatcta tcgagttaga gatttaatct aacttactag
     2881 agtcggtaca ataacagtct cagcttttta ttgtgcagta tatacacatt tttattttag
     2941 tatttattta aaagtttctg ctaaaaatga ttcaacttgt tcaggtgact tagcatttgc
     3001 tgaatgaagg tgtgcaattt tatcgccgtt tttaaatact agcaagctag ggatacccat
     3061 aacttcattt tcaacaacta catcttctaa ttcatcacga ttaacagtat accattggta
     3121 atcattatat tgttctacga ttgggtcaat ccataaatcc atagcacgac agtctgggca
     3181 ccatcctgcc tcaaatttaa caattacagg tgtatcgcta ttaattacag atttaaatga
     3241 ttcattactt ttgattgatt gcattgtaac aactcctcta gatagtttaa taatttttat
     3301 tatagctaaa tttatatcat aataaaaaat tttagcttca aaatgaaagc cttttgcttt
     3361 ggaaaatgat atatttattt taaatacata aaggaggttg cagtcgtatg attaaatttt
     3421 accaatataa gaattgtaca acttgtaaaa aggcagcaaa gtttttagat gaatatggcg
     3481 taagttatga accaattgat atcgttcaac atacacctac aataaatgaa tttaaaacaa
     3541 taattgcaaa tacaggcgta gaaattaata aattgtttaa tacacacggc gcgaaatatc
     3601 gtgagcttga tttgaaaaat aaattacaaa ctttatcaga tgatgaaaag ttagagttgt
     3661 tatcatctga tggtatgtta gtaaagcgtc ctctagcagt aatgggcgat aagataacat
     3721 taggatttaa agaagatcaa tataaagaga cttggttagc gtaagtgaaa tgtaagcgtt
     3781 tactaaatat ctcgatattt agattcatta catgtaaaat gaaataagct atacaattgt
     3841 taatttttat aaatatagtt gaatagcatc tagccttatg gcatcattaa tgatgtaaag
     3901 attaattagg aggggattct cttggcagta ccaaatgaat tgaaatattc aaaagagcat
     3961 gaatgggtta aagttgaagg taatgtagca ataattggaa tcacagaata cgcacaaagc
     4021 gagttaggtg atattgtttt cgttgaatta ccagaaacag atgatgaaat taatgaaggg
     4081 gatacgtttg gtagcgtaga atcagttaaa actgtatcag aattatatgc accaatctct
     4141 ggtaaagtag ttgaagtcaa cgaagaacta gaagatagtc ccgaatttgt aaatgaatct
     4201 ccatacgaaa aagcatggat ggtaaaagta gaaattagtg atgaaagtca gattgaagct
     4261 ttattaacag ctgaaaaata ttcagaaatg attggtgaat aatcaccgtg taactcctta
     4321 atctaagatt gaggagttta tttttagtct gagaaaataa atgatatgaa agaaaaatta
     4381 ttaggtacta ttatttggag tattgctaca ttttattatt caagaatgat ggaaataatg
     4441 aatttagcta ttttaaaaat aaaaattggg ggaagttaat atgctaaaca ttcaagacgt
     4501 tagtcatctt tctaaaaagg agcaaaaagc atataaccgt ttcgtagaat ctgtagaaaa
     4561 cggtaattta ccagtactac catgtattga aatggatcta aaagagatgc aagaagaaac
     4621 attaaaccag agtaagattg gtggaatgcc atttttaaaa tattttaaag atataccatt
     4681 agatgaaaat aatgtaccaa tggtattgct agcacagatt aatttggatg atcttccaga
     4741 acaacaagaa ttatttcctg taaaagaagg gatattgcag ttttggatta gttcagaaga
     4801 tcaaatgtat ggaatgtctg aaaatttaaa gggaaacaat ataaactcaa ggcttgttta
     4861 tataaaagag ccaattacag atttatcact cgaaaatatt caagcgcatt tgaagtcatt
     4921 agatgctgat aatgaggata tcccgttcag tggagcattt tctatagaat ttagattgtc
     4981 gaaacaaact attacatgta ctgattataa gtacgatgag gacgtgcttg cattgtggaa
     5041 taaagtcaat ccatccttcg cgctaaaatc aatgtttggt ggttatgatg aattgatgga
     5101 acctgtgtgt aacacattta ctgctaagga accatttaat caacttggtg gttatccata
     5161 ttttgaccaa atagatccaa gaacgaacga tcaagaactg aaaatgtatg atagagtctt
     5221 actgcaaatt gattctacaa gagatggtaa ttcttcgatt atatggggtg atttaggtat
     5281 tgccaatatc ttagtgaaat ctactgacct tgaggctatg aagtttgatg attacatgta
     5341 ttcatgggat tgcagctaag agaatcataa aaatatatta ctttaataag tgtagtatct
     5401 atagatcaat ctagggagtg ggaca
//
LOCUS       DALAGD010000134         5382 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.137, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000134 DALAGD010000000
VERSION     DALAGD010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5382
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.137"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..353
                     /gene="fmhC"
                     /locus_tag="O2701_002529"
     CDS             <1..353
                     /gene="fmhC"
                     /locus_tag="O2701_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhC"
                     /protein_id="HCZ4294395.1"
                     /translation="TEELIFEDGPVLDLAAALFICTDDEVYYLSSGSNPKYNQYMGAY
                     HLQWHMIKYAKSHNINRYNFYGITGVFSNEADDFGVQQFKKGFNAHVEELIGDFIKPV
                     RPILYKFAKLIYKV"
     gene            526..1398
                     /gene="dprA"
                     /locus_tag="O2701_002530"
     CDS             526..1398
                     /gene="dprA"
                     /locus_tag="O2701_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCZ4294396.1"
                     /translation="MIKLFLLKLYWAHFSTKQIHQFLMAYPNVIKEEGRKKDSYLCEW
                     VNREENVHLLRKYYAFIKLDHNDIIKELQKLKVSYITYMDSEYPVLLKEIYQFPLLLF
                     YKGNIKLINNMHHLAVVGARDSTSYTQQSLEFLLSNDKSKYLTIVSGLAQGADAMAHQ
                     IALKYNLPTIAVLAFGHQTHYPKSTLALRNKIEEKGLVISEYPPHTPIAKYRFPERNR
                     IISGLSKGVLITEAKEQSGSHITIDFALEQNRNVYVLPGSMFNPMTKGNLLRIQEGAK
                     VVLNANDIFEDYYI"
     gene            1572..3647
                     /gene="topA"
                     /locus_tag="O2701_002531"
     CDS             1572..3647
                     /gene="topA"
                     /locus_tag="O2701_002531"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCZ4294397.1"
                     /translation="MTLADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQM
                     GVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILEL
                     EDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKV
                     KKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPF
                     KLKTKKDVEKITAALDGDQFEITNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKT
                     MMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKVEAKQYITDKYGESYTSKRKA
                     SGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVS
                     LDITQGDIKFRANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPA
                     QHYTQPPPRYTEARLVKTLEELKIGRPSTYAPTIDTIQKRNYVKLESKRFVPTELGEI
                     VHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERAEEEME
                     KIEIKDEPAGEDCEICGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCND
                     GDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSN
                     CDYKEAAQK"
     gene            3803..5110
                     /gene="trmFO"
                     /locus_tag="O2701_002532"
     CDS             3803..5110
                     /gene="trmFO"
                     /locus_tag="O2701_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HCZ4294398.1"
                     /translation="MTQTVNVIGAGLAGSEAAYQLAERGIKVNLIEMRPVKQTPAHHT
                     DKFAELVCSNSLRGNALTNGVGVLKEEMRRLNSIIIEAADKARVPAGGALAVDRHDFS
                     GYITETLKNHENITVINEEINAIPDGYTIIATGPLTTETLAQEIVDITGKDQLYFYDA
                     AAPIIEKESIDMDKVYLKSRYDKGEAAYLNCPMTEDEFNRFYDAVLEAEVAPVNSFEK
                     EKYFEGCMPFEVMAERGRKTLLFGPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNI
                     VGFQTHLKWGAQKEVIKLIPGLENVDIVRYGVMHRNTFINSPDVLNEKYELISQPNIQ
                     FAGQMTGVEGYVESAASGLVAGINLAHKILGKGEVVFPRETMIGSMAYYISHAKNNKN
                     FQPMNANFGLLPSLETRIKDKKERYEAQANRALDYLENFKKTL"
BASE COUNT         2056 a          759 c          981 g         1586 t
ORIGIN      
        1 aaaccgaaga actaatattt gaagatggac ctgttttgga tttagctgct gctttattta
       61 tatgtactga tgatgaagtt tattatctat caagtggatc aaatccgaaa tataatcagt
      121 atatgggtgc atatcatcta caatggcata tgataaaata tgcaaaatca cataatatta
      181 ataggtataa tttttatgga ataacaggcg tctttagtaa tgaggcggat gattttggtg
      241 ttcaacaatt taaaaagggt tttaatgcac atgttgaaga attaattggt gatttcatca
      301 aaccagtaag accaattcta tataaatttg caaaacttat ttataaggtt taattataaa
      361 gtatgttgga aattgaaatt ttaaattctt tccaacatac ttttcacttt ttaagtcagt
      421 atttttactc cttaaaataa cattacatcg acaatgtttt tgtattagaa atgtaaaaca
      481 aaaatagaat atgattttgt atgattacaa aaaggagttg atttattgat taaactattt
      541 ttgcttaagt tatactgggc acacttttcg actaaacaaa ttcatcaatt tttaatggca
      601 tatcctaatg taattaaaga ggagggaaga aaaaaagata gttatttatg tgaatgggtg
      661 aatagggaag aaaatgttca tttattacgt aaatactatg cttttataaa acttgatcat
      721 aacgatatta ttaaagaact gcagaaatta aaagtaagtt acattacata tatggattct
      781 gaatacccag tgctattaaa agaaatatat caatttccat tacttctttt ctataaaggg
      841 aacatcaaat taataaataa tatgcatcat ttggcagtag taggtgcaag agattctaca
      901 agttataccc aacagtcttt agaattttta ttatcaaatg ataaaagcaa atatttaaca
      961 attgtttccg gccttgctca aggagctgat gcaatggcac atcaaatagc tttaaaatac
     1021 aatctcccta caattgcagt tttagccttt ggccatcaaa cacattatcc caaaagtaca
     1081 ttagcattaa gaaataaaat agaagaaaaa ggtttagtta tatctgaata tccaccacat
     1141 acaccaatcg ctaaatatag atttcctgag cgcaatagaa ttatcagcgg tttgtcaaaa
     1201 ggggttttaa ttactgaggc taaggaacaa agtggcagtc acatcacgat agattttgca
     1261 ttagagcaaa atagaaatgt ttatgtttta cctggatcta tgtttaatcc tatgacaaaa
     1321 ggtaatttat tacgtatcca agaaggtgct aaggtagtat taaacgctaa tgatatattt
     1381 gaagactact atatttaaaa ctaaaaataa cgagttgctt catatttctt ttgtttccct
     1441 ttgtaattta ttttttaata aaatctataa aaaatagaca gggaaaatga tttgtttaga
     1501 tataaaacgt tgacaaaagc aaaattaagc gtttatcatt tatctttagt aattagatta
     1561 gcgaggggga aatgacattg gcagataatt tagtcattgt tgaatcgcct gcaaaagcaa
     1621 aaaccattga aaagtattta ggtaagaaat ataaagttat agcttcaatg ggacacgtca
     1681 gagacttacc aagaagtcaa atgggtgtcg acactgaaga taattacgaa ccaaaatata
     1741 taacaatacg cggaaaaggt cctgttgtaa aagaattgaa aaaacatgca aaaaaagcga
     1801 aaaacgtctt tctcgcaagt gaccccgacc gtgaaggtga agcaattgct tggcatttat
     1861 caaaaatttt agagcttgaa gattctaaag aaaatcgcgt tgttttcaac gaaataacta
     1921 aagacgctgt taaagaaagt tttaaaaatc ctagagaaat tgaaatgaac ttagtcgatg
     1981 cacaacaagc gcgtcgaata ttagatagat tagttggcta taacatctcg ccagttcttt
     2041 ggaaaaaagt aaaaaaaggg ttgtcagcgg gtcgagttca atctgttgca cttcgtttag
     2101 tcattgaccg tgaaaatgaa attcgaaact ttaaaccaga agaatattgg actattgaag
     2161 gagaatttag atacaaaaaa tcaaaattca atgctaaatt ccttcattat aaaaataaac
     2221 cttttaaact aaaaacgaaa aaagatgttg agaaaattac agctgcatta gatggagatc
     2281 aattcgaaat tacaaacgtg actaaaaaag aaaaaacgcg taatccagca aacccattta
     2341 caacttctac attacaacaa gaggcggcac gtaaattaaa ctttaaagca agaaaaacaa
     2401 tgatggtcgc acaacaatta tatgaaggta tagatttgaa aaaacaaggt acgattggtt
     2461 taataacata tatgagaacc gattctacac gtatttcaga tactgccaaa gttgaagcaa
     2521 aacagtatat aactgataaa tacggtgaat cttacacttc taaacgtaaa gcatcaggga
     2581 aacaaggtga ccaagatgcc catgaggcta ttagaccttc aagtactatg cgtacgccag
     2641 atgatatgaa gtcatttttg acgaaagacc aataccgatt atacaaatta atttgggaac
     2701 gatttgttgc tagtcaaatg gctccagcaa tacttgatac agtctcatta gacataacac
     2761 aaggtgacat taaatttaga gcgaatggtc aaacaatcaa gtttaaagga tttatgacac
     2821 tttatgtaga aactaaagat gatagtgata gcgaaaagga aaataaactg cctaaattag
     2881 agcaaggtga taaagtcaca gcaactcaaa ttgaaccagc tcaacactat acacaaccac
     2941 ctccaagata tactgaggcg agattagtaa aaacactaga agaattgaaa attgggcgac
     3001 catcaactta tgcaccgaca atagatacga ttcaaaagcg taactatgtc aaattagaaa
     3061 gtaagcgttt tgttcctact gagttgggag aaatagttca tgaacaagtg aaagaatact
     3121 tcccagagat tattgatgtg gaattcacag tgaatatgga aacgttactt gataagattg
     3181 cagaaggcga cattacatgg aggaaagtaa tcgacggttt ctttagtagc tttaaacaag
     3241 atgttgaacg tgctgaagaa gagatggaaa agattgaaat caaagatgag ccagccggtg
     3301 aagactgtga aatttgtggt tctcctatgg ttataaaaat gggacgctat ggtaagttca
     3361 tggcttgctc aaacttcccg gattgtcgta atacaaaagc gatagttaag tctattggtg
     3421 ttaaatgtcc aaaatgtaat gatggtgacg tcgtagaaag aaaatctaaa aagaatcgtg
     3481 tcttttatgg atgttcgaaa tatcctgaat gcgactttat ctcttgggat aagccgattg
     3541 gaagagattg tccaaaatgt aaccaatatc ttgttgaaaa taaaaaaggc aagacaacac
     3601 aagtaatatg ttcaaattgc gattataaag aggcagcgca gaaataatat ttttatttcc
     3661 tagatacatt ttaagattgt taaatagaat cattagtgaa tcttatttta aagatagtaa
     3721 aggattaatc taaataagtg cggataatat aaacataaca acataattaa aagacataaa
     3781 tgacaataaa aggagtatag aaatgactca aactgtaaat gtaataggtg ctggtcttgc
     3841 cggttcagaa gcggcatatc aattagctga aagaggaatt aaagttaatc taatagagat
     3901 gagacctgtt aaacaaacac cagcgcacca tactgataaa tttgcggaac ttgtatgttc
     3961 caattcatta cgcggaaatg ctttaactaa tggtgtgggt gttttaaaag aagaaatgag
     4021 aagattgaat tctataatta ttgaagcggc tgataaggca cgagttccag ctggtggtgc
     4081 attagcagtt gatagacacg atttttcagg ttatattact gaaacactta aaaatcatga
     4141 aaatatcaca gttattaatg aagaaattaa tgccattcca gatggataca caattatcgc
     4201 aacaggacca cttactacag aaacccttgc gcaagaaata gtggacatta ctggtaaaga
     4261 tcaactttat ttctatgatg cggctgctcc aattattgaa aaagaatcta ttgatatgga
     4321 taaagtttac ttaaagtccc gttatgataa aggtgaagct gcatatttaa actgtcctat
     4381 gactgaggat gaatttaatc gcttttatga tgcagtatta gaagctgaag ttgcgcctgt
     4441 aaattcattt gaaaaagaaa aatatttcga gggttgtatg ccttttgaag taatggcaga
     4501 acgcggacgc aagacattac tatttggacc aatgaaacca gtaggattag aagatccaaa
     4561 gactgggaaa cgtccttatg cggtggttca attaagacaa gatgacgctg ctggtacact
     4621 ctacaatatt gttggcttcc aaacgcattt aaaatgggga gctcaaaaag aagtcattaa
     4681 attaattcca ggcttagaaa atgttgatat tgttagatat ggtgtgatgc atagaaatac
     4741 cttcattaat tcaccggacg tattaaacga gaaatatgaa ttgatttcac aaccaaacat
     4801 acagtttgcg ggacaaatga ctggtgttga aggttatgta gaaagcgcag ctagcggctt
     4861 agttgcaggt atcaatcttg cgcataaaat attaggcaag ggtgaggtag tatttccgag
     4921 agaaacaatg attggaagta tggcttacta tatttctcat gctaaaaaca ataagaattt
     4981 ccaacctatg aatgctaact tcgggttatt accatcttta gaaactagaa ttaaagataa
     5041 aaaagaacgc tatgaagcac aagctaatag agctttggat tacttagaaa atttcaaaaa
     5101 aactttataa aatagttaga aagactagat atgctattca ttcttaagtc atcaacgagt
     5161 aagtaatgac tttctaaatg gaaaatactt atcctagtct ttttaatttt ggaattgtta
     5221 cgtatttctg acaatttaga attcgcattc aaaaaatatc taaataaata acacgcaata
     5281 agttgattga tgtaacatgt aagagaatgt tttaaataaa ctttatttaa aaggcaatga
     5341 aataataaat ggcaaggcta ttaataaaga cttttagtaa tt
//
LOCUS       DALAGD010000135         5341 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.138, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000135 DALAGD010000000
VERSION     DALAGD010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.138"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..724
                     /locus_tag="O2701_002533"
     CDS             <1..724
                     /locus_tag="O2701_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294399.1"
                     /translation="MYSKVFEQYKKNVVLDKVYYSEVYKYSDNLIKVFSEDVWNIMGY
                     KKYNEYFTNRDDTTLKITNLYIGQSFGTEGNRNVIDRIGKGHEKLQEIMTYQPENRKT
                     VLIFYKIQPDNIYITNQYGKEAVSRLKRILEQSIPNKEYIDLSEISLITFFKPEYNKQ
                     HVNEEFNSLSSSKIKNLAKENDGIIIYLNFKDVNYQLISEGNTFFSAKPYLHCVFNKD
                     ILNMDKVDIENFFKSIEVKYSK"
     gene            1278..1460
                     /locus_tag="O2701_002534"
     CDS             1278..1460
                     /locus_tag="O2701_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294400.1"
                     /translation="MKDSIFWKKAFIPVYFIVAMLVFLLFRFYIKTDNFSIYLMSIFL
                     ICLGTASIIYNYKTNR"
     gene            complement(1442..1681)
                     /locus_tag="O2701_002535"
     CDS             complement(1442..1681)
                     /locus_tag="O2701_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294401.1"
                     /translation="MCVVIMRDVGLIDVEDCYDIFGMWCLVNGGLKLSFYGGLKMLFY
                     VGLEFVVLWRYKKPQQDLDLTVTYTFHTYIYRLVL"
     gene            1914..3578
                     /gene="alsS"
                     /locus_tag="O2701_002536"
     CDS             1914..3578
                     /gene="alsS"
                     /locus_tag="O2701_002536"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HCZ4294402.1"
                     /translation="MTDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGP
                     ELIVTRHEQNAAMMAQGIGRLTGKPGVVLVTSGPGVSNLTTGLLTATSEGDPVLALGG
                     QVKRNDLLRLTHQSIDNAALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKNGASFI
                     SIPQDVISSPVESKAISLCQKPNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSAEE
                     TNAIRKLVERTNLPVVETFQGAGVISRELENHFFGRVGLFRNQVGDELLRKSDLVVTI
                     GYDPIEYEASNWNKELETQIINIDEVQAEITNYMQPKKELIGNIAKTIEMISEKVDEP
                     FINQQHLDELEQLRTHIDEETGIKATHEEGILHPVEIIESMQKVLTDDTTVTVDVGSH
                     YIWMARNFRSYNPRHLLFSNGMQTLGVALPWAISAALVRPNTQVVSVAGDGGFLFSSQ
                     DLETAVRKNLNIIQLIWNDGKYNMVEFQEEMKYKRSSGVDFGPVDFVKYAESFGAKGL
                     RVTNQEELEAAIKEGYETDGPVLIDIPVNYKDNIKLSTNMLPDVFN"
     gene            3615..4319
                     /gene="budA"
                     /locus_tag="O2701_002537"
     CDS             3615..4319
                     /gene="budA"
                     /locus_tag="O2701_002537"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCZ4294403.1"
                     /translation="MTNVLYQHGTLGTLMAGLLKGTASINELLQHGDLGIATLTGSNG
                     EVIFLDGKAYHANEHKEFVELKGDELTPYATVTKFVADTSYETKDKSSEAVFAEIKEK
                     MLSENLFSAVKISGLFKKMHVRMMPAQEPPYTRLIDSARRQPEQTETYVKGSVVGFFT
                     PELFHGIGSAGFHVHFANDDRNFGGHVLDFEVEDVKVEIQNIETFEQHFPIQDKDFTK
                     ANIDYKDIADEIREAE"
     gene            complement(4683..5108)
                     /locus_tag="O2701_002538"
     CDS             complement(4683..5108)
                     /locus_tag="O2701_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HCZ4294404.1"
                     /translation="MKLKSFVTATLALGLLSTVGAALPSHEASADSNNGYKEMTVDGY
                     HTVPYTISVDGITALHRTYFIFPENKNVLYQEIDSKVKNELASQRGVTTEKINNAQTA
                     TYTLTLNDGNKKVVNLKKNDDAKNSIDPSTIKQIQIVVK"
BASE COUNT         1943 a          748 c          906 g         1744 t
ORIGIN      
        1 tatgtactca aaagtatttg aacaatataa aaaaaacgtt gtattggata aagtatatta
       61 ctctgaagtt tataaatata gtgataattt gataaaagta ttctctgaag acgtttggaa
      121 cataatgggg tataaaaaat ataatgaata ttttacaaac agagatgata ctacattaaa
      181 aattactaat ttatatattg ggcaatcttt tggtacagag ggtaatagaa atgtcattga
      241 tagaataggg aaaggtcatg aaaaattaca agaaattatg acttatcagc cagaaaatag
      301 aaagactgtc ttaatatttt acaagataca gcctgacaat atttatatta caaaccaata
      361 tggtaaagag gctgtgtctc gattaaaaag aattttagag caaagtatac ctaataaaga
      421 atatattgat ttatcagaga tatcattaat tacatttttt aaaccagaat ataataaaca
      481 acatgtgaat gaggagttta attctttatc ttcttcgaaa ataaagaatt tagccaaaga
      541 aaatgatggt attattattt atttaaattt taaggatgtt aattatcaac tgataagtga
      601 aggcaatact tttttttctg caaaaccata tttgcattgt gtgttcaata aagatatttt
      661 aaatatggat aaagttgata tagaaaattt tttcaaaagt atagaagtca aatacagtaa
      721 ataagatcaa ttattgaatc caccgaccag cagatttttt taaaaaatta ataccccgtt
      781 aatacccttt gcttcaattt gatgaaaatc aatgaaattc aaaattgaag aaatccttta
      841 atatcaaggt gtacgacagt ctatataatc atgcgaaatt ctaaaatttt ctgaacgtaa
      901 aaaaacaaat tcttaaagca gctcgtcgtt cacctcaatt ctcaaaacgt taattgtcgg
      961 acgatatata tacaaaacac ctcgatgtta tgtcgaggtg tttttttgcg tttgtgtggg
     1021 gaatatggaa tagagtataa atgaattaat gtctcaggta tagaattaat tcaactatga
     1081 attattgatt tacacgtata atttgtacat gcttacacaa tcaaaagtgt acattattaa
     1141 attgtcattt agcttgcgga gaccgtacga tagtgttcta ataatagata ttcaagggaa
     1201 agagctatcg gatgataacc agatgataac tcaagaacca tgatttaaga gtgttatcaa
     1261 aataaggagt gacttaattg aaagattcta tattttggaa gaaagctttt attcctgttt
     1321 attttattgt tgcgatgctg gtgtttctac tttttaggtt ttatattaaa acagataact
     1381 tttctatata tttaatgagt atcttcttaa tttgtttagg aactgcttct atcatttata
     1441 actataaaac caatcgataa atataagtat gaaatgtata agtcacagtt agatctaagt
     1501 cttgctgtgg ctttttatat ctccataaaa cgacaaattc aagcccgaca taaaacagca
     1561 ttttcagccc accataaaac gacaatttca gcccgccatt gactaaacac cacatcccaa
     1621 aaatatcgta acaatcctct acatcaatca atccaacatc cctcataatc acaacgcaca
     1681 aaatctattc atgcattttt ggaatactta gtattacaaa taacgatttt tattcatcta
     1741 acaaaggtta tataatgtac tgaaggcaat ttttatgtat tacaaatcta atcgtacatg
     1801 taaaattttg ataaacatca ttaattttgc gtaactatca ttagattaca aatcacaaag
     1861 taattacatg taatacacat ctatacatca catttgaagg gaaatgaata taaatgactg
     1921 ataaaaagta cactgcagcc gatatggtta ttgatacttt gaaaaataat ggggtagaat
     1981 atgtttttgg tattccgggt gcaaagattg actatctatt taatgcttta attgatgatg
     2041 gtcctgaact tattgtcact cgtcatgaac aaaacgccgc aatgatggcg caaggtattg
     2101 gaagattaac aggcaaaccg ggtgtagtac ttgttacaag tggtcctggt gtaagtaatt
     2161 taactactgg attattaacc gcaacatctg aaggggatcc tgtattagcg ttaggtggcc
     2221 aagtgaaacg taatgattta ttacgattaa cgcatcaaag tattgataat gctgcgctat
     2281 taaaatattc atcaaaatat agtgaagaag tacaagatcc tgaatcatta tcagaagtta
     2341 tgacaaatgc aattcgaatt gctacttcag gaaaaaatgg cgcaagtttt attagtattc
     2401 cgcaagacgt tatttcttca ccagttgaat ctaaagctat atcactttgc caaaaaccaa
     2461 atttaggagt accgagtgaa caagatatta atgatgtcat tgaagcgatt aaaaatgcat
     2521 catttcctgt tttattagct ggtatgagaa gttcaagtgc agaagaaaca aatgccattc
     2581 gcaaattagt tgagcgcacg aatttaccag ttgtagaaac attccaaggt gcaggtgtaa
     2641 ttagtcgtga attagaaaat catttcttcg gtcgtgtggg cttattccgc aatcaagttg
     2701 gtgatgaatt attacgtaaa agtgatttag ttgttacaat cggttatgat ccaattgaat
     2761 acgaagctag taactggaat aaagaattag aaacacaaat tatcaatatt gacgaagttc
     2821 aagctgaaat tactaattat atgcaaccga aaaaagagtt gattggtaat attgctaaaa
     2881 cgattgaaat gatttctgaa aaagtggatg agccatttat aaatcaacaa catttagacg
     2941 aattagaaca attaagaaca catattgatg aagaaactgg tattaaagcg acgcatgaag
     3001 aaggaattct acatccagtg gaaattattg aatctatgca aaaggtatta actgatgata
     3061 ctactgtaac agttgatgtt ggaagtcact atatttggat ggcacgtaat ttcagaagtt
     3121 acaatccaag acatttatta tttagcaatg gtatgcaaac gcttggtgta gcattaccgt
     3181 gggcaatttc agctgcactt gtgcgcccta atacgcaagt tgtgtccgtt gctggcgatg
     3241 gtggcttttt attttcatca caagatttag aaacggccgt acgtaaaaat ttaaatatca
     3301 tccagcttat ttggaatgat ggaaaatata acatggttga attccaagaa gaaatgaaat
     3361 ataaacgttc gtcaggtgta gacttcggtc ctgtagattt tgtaaaatat gcagaatcat
     3421 ttggcgcgaa aggtttacga gttactaatc aagaagaatt agaagcggca attaaagagg
     3481 gctatgaaac agatggtcca gtattaattg atatacctgt aaattacaaa gataatatca
     3541 aactttcaac aaatatgtta cctgacgtat ttaactaaaa taaagataaa tgttaaagag
     3601 gagtgggaga ttttatgact aatgttttat accaacatgg tacattaggc acattaatgg
     3661 caggattatt aaaaggaact gcatcaataa atgaattatt gcaacatggt gacttaggta
     3721 tcgctacact aacaggttca aacggtgagg taatcttttt agatggaaaa gcttaccatg
     3781 caaatgaaca taaagaattt gtagaattaa aaggtgacga gttaacacca tatgcaactg
     3841 taactaaatt tgtagcagat acaagctatg aaacgaaaga taaatcttca gaagcagttt
     3901 ttgcagaaat taaggaaaag atgttgagtg aaaatttatt ttcagcagta aaaatttcag
     3961 gcttatttaa aaaaatgcat gtacgtatga tgccggctca agaaccacct tatacacgtt
     4021 taatcgattc agctagaaga caacctgaac aaactgaaac gtatgtcaaa ggttcagtcg
     4081 ttggtttctt tacaccagaa ttattccatg gtatcggatc agcaggattt catgtacact
     4141 ttgcgaatga tgatcgtaac tttggtggac atgtcttaga ttttgaagta gaagatgtta
     4201 aagtagaaat ccaaaatata gaaacatttg aacagcattt tccaattcaa gataaagatt
     4261 tcactaaagc aaatattgac tataaagata ttgcagacga aattagagaa gctgaataat
     4321 gaatccagaa atataatgac ggtttatgaa aattgacttc ataatgcgcg atttagaaat
     4381 gatagtttgt aaatatgatt aaccatgact acaatagaac aaatatattt ataattacgt
     4441 ctaagtaata aaataaatcc cttcactatt agcagtagtg aggggattta ttaggttcca
     4501 gatatttgag atttgctgtt atgtttagat tataaattgt ggtacacact catataaaat
     4561 ttactattgt ataggccaat ctgttactac gagaagcaaa caacaataat ttacaagttc
     4621 aataactaaa aagacaaacg ccaatttttc agcgcttgcc ctataaaact attttcaaat
     4681 tattatttaa ctacaatttg tatctgtttg attgtacttg gatcaattga atttttagcg
     4741 tcgtcatttt tctttagatt cactactttt ttattaccat cattcaaagt aagcgtataa
     4801 gttgctgttt gggcattatt aattttttct gttgtaacac cacgttgaga agctaattca
     4861 ttttttactt tactgtcaat ttcttgataa agaacatttt tattttctgg gaagataaag
     4921 taagttcgat gtaatgcagt aataccatct actgaaattg tgtaaggaac agtgtgataa
     4981 ccatccacag tcatttcttt atagccgtta ttactatctg cagatgcttc gtgactcggt
     5041 aatgcagctc cgaccgttga taataatccc aatgctaaag tggcagtaac aaatgatttt
     5101 agtttcatat ctcaatctcc ttttcgggtt gtatgatatt acactatttg tataacacgt
     5161 ttcataaagt ttaaaacata ctttttgcat tttttatatt ttaattttta gtaatagttt
     5221 ttaacgaggg atatgactcg tgcattttga aaagtggtaa gcaatgtttt tagtttatag
     5281 ggattgatta gttgtatata ataatcatga aaagcatata gtcgatctta tacttttaat
     5341 t
//
LOCUS       DALAGD010000136         5308 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.139, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000136 DALAGD010000000
VERSION     DALAGD010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.139"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(16..1608)
                     /gene="pckA"
                     /locus_tag="O2701_002539"
     CDS             complement(16..1608)
                     /gene="pckA"
                     /locus_tag="O2701_002539"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCZ4294405.1"
                     /translation="MSVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGVLTE
                     LGAVNASTGKYTGRSPKDKFFVSEPSYRDNIDWGEINQPIDEETFLKLYHKVLDYLDK
                     KDELYVFKGYAGSDKDTMLKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFTIVS
                     APHFKADPEVDGTKSETFVIISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQDIMS
                     MHCSANVGEKGDVALFFGLSGTGKTTLSADPHRKLIGDDEHGWNKNGVFNIEGGCYAK
                     AINLSKEKEPQIFDAIKYGAILENTVVAEDGSVDFEDNRYTENTRAAYPINHIDNIVV
                     PSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSF
                     STCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMV
                     NQAISGKLKNAEYTKDSTFGLSIPVEIEDVPKTILNPINAWSDKEKYKAQAEDLIQRF
                     EKNFEKFGEKVEHIAEKGSFNK"
     regulatory      1763..1873
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1979..3172
                     /locus_tag="O2701_002540"
     CDS             1979..3172
                     /locus_tag="O2701_002540"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCZ4294406.1"
                     /translation="MLNNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVAC
                     ETTVTTGMALIAGEISTTTYVDIPKVVRETIKEIGYTRAKYGYDYETMAILTAIDEQS
                     PDIAQGVDKALEYRDKDSEEEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKR
                     LSDVRKDGTLNYLRPDGKVQVTVEYDENDNPVRIDTIVVSTQHAEDVTLEQIQEDIKA
                     HVIYPTVPENLINEQTKFYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCF
                     SGKDPTKVDRSAAYAARYVAKNIVAAGLADQCEVQLAYAIGVAEPVSIAIDTFGTGKV
                     SEGQLVEAVRKHFDLRPAGIIKMLDLKQPIYKQTAAYGHFGRTDVLFPWEKLDKVEEL
                     KDAVK"
     gene            3297..4205
                     /locus_tag="O2701_002541"
     CDS             3297..4205
                     /locus_tag="O2701_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HCZ4294407.1"
                     /translation="MDLSSPIVIGLIIAVVIALIFFVLFLVALGSKKKVKRQTEEKYE
                     QQEQNIKKSHEEALEKERIQNKKTITKQQEDYNHMVSTKDREIDALKLFSKNHSEYVT
                     DMRLIGIRERLVKEKRIRPEDMHIMANIFLPKDGFNNIERISHLVLTRTGLYIIDSQL
                     LKGHVYNGISGGQFKDLPPMEQVFDTLDLDKSRPQTIVMDQNDDKRSLSFVNYSDQIE
                     AIKQLAEDLQKELGAKYTPTSILYFNLKNEGDVTISNYNQNSAVKVLVGAEQLDEFFN
                     KFVFHGRIQYNVEDLQQMMDKIESFN"
     gene            4417..5250
                     /locus_tag="O2701_002542"
     CDS             4417..5250
                     /locus_tag="O2701_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCZ4294408.1"
                     /translation="MEVKTFYNGNTMPQIGLGTFRVENDENCMESVKYAIEQGYRSID
                     TAKVYGNEEQVGAGIRAGLESTGIAREDLFITSKLYFEDFGRENVAAAYEASLSRLGL
                     KYLDLYLVHWPGTNEAVMVDTWKGMEDLYKNNNVKNIGVSNFEPEHLEALLAQVSIKP
                     VINQVEYHPYLTQHKLKLYLAAQRIVMESWSPLMNAQILNDETIKDIAQELGKSPAQV
                     VLRWNVQHAVVTIPKSVTPNRISENFQIFDFELSDEQMTRIDGLNQDKRIGPDPKKFE
                     G"
BASE COUNT         1807 a          813 c          985 g         1703 t
ORIGIN      
        1 atttagtatt caaatttatt tgttgaagct acctttttca gcaatatgtt caactttttc
       61 accaaatttt tcgaagttct tttcaaaacg ttgaattaaa tcttctgctt gtgctttata
      121 tttctctttg tcgctccaag cattaattgg atttaaaatt gttttcggta catcttcaat
      181 ttctacagga atgcttaaac caaacgtact atcttttgta tattctgcat ttttcaattt
      241 gccagaaatc gcttggttta ccatttgacg tgtgtaatgt aagctgattc tacgtcctac
      301 accatatttt ccgccagtcc atccagtatt aacaagataa acatcaacat catgtaaatc
      361 gataagttca cctaatagat cagcgtaaac agtagggtgt aacgggaaga acggtgctcc
      421 gaaacatgtt gagaatgatg gttcaggttc tgtcacacca cgctctgtac cagctaattt
      481 agaagtgaaa ccactcaaga aatgatacat tgcttggtct ttatttaact ttgaaatcgg
      541 tggaataaca ccaaatgcat ccgcagttaa gaaaataatt gtatttggat gtgctgcttt
      601 agatggtact acaatattgt caatgtgatt aattggataa gcggcacgcg tgttttctgt
      661 ataacgattg tcttcaaagt ccactgaacc atcttctgca actacagtgt tctctaaaat
      721 tgcaccatat ttgattgcgt caaaaatctg tggttctttt tctttggaaa gattaattgc
      781 ttttgcatag cagccacctt cgatattaaa gaccccgttt ttattccagc cgtgttcatc
      841 atcaccgatt agtttacggt gtgggtcagc cgataaggtt gttttaccag tgccagatag
      901 accaaagaat aatgcaacat cgcctttttc accaacgttt gctgagcaat gcatgctcat
      961 aatatcttgc atcggtaaga gataattcat tacagagaag ataccttttt tcatttcacc
     1021 agcgtattca gtaccgccga ttaaaatgac tttgtgttta aatgaaataa tgacaaaggt
     1081 ttcagattta gtaccatcaa cttctggatc tgctttaaaa tgtggtgcag aaacgatagt
     1141 gaagttaggt ttaatctttg tagcttcttc ttttgattca ggtctaataa acatattttt
     1201 agcaaataaa ttatgccatg ctaattcatt gatgactgta agttttaaca ttgtatcttt
     1261 atcgctacca gcgtagcctt taaatacgta tagttcatct tttttatcta aatagtctag
     1321 tactttatgg tataacttca agaaagtttc ttcatcgata ggttgattaa tttctcccca
     1381 atcaatgtta tctctatatg aaggttcaga gacaaaaaat ttgtctttag gcgaacgacc
     1441 agtatatttt ccagtacttg cattaacagc accaagttct gttaataccc cttcgttatt
     1501 gtctaagatt ttattataaa gttgtgtcgt cgaaagttga aaatgtgacg ttggtttttt
     1561 cagtaatttg tcaattttag ttgtttcagt gtatgtgtct actgacatcc taaatccctc
     1621 caaagcgata atttaaatgt aagcctttac atttaattag tataacacat taacataatt
     1681 aatctacaat attttaccgt aaatttattt gtgaaaaatt gacattaatt cattaattag
     1741 gtactattat ttttaacgga ttctcttatc ctgagtggtg gagggacatg gacccaatga
     1801 aacccagcaa cctctttttt ataaaagaaa ggtgccaaac cgtttgcaga caaataggtc
     1861 tgaacgataa gagcgaatgg acgtattagg ccttctctct atattaatag ttagaaggtc
     1921 ttttttattt agctcacaga gagagaattt tcgtaatata aatttaaagg agcaaactat
     1981 gttaaataac aaacgattat ttacttcaga gtctgttaca gaaggacacc cagataaaat
     2041 cgctgaccaa gtgtcagatg caatattaga tgctatttta aaagacgatc caaatgcacg
     2101 tgtagcttgt gaaacaactg ttacaacagg tatggcattg atcgccggcg aaatttctac
     2161 aacaacatat gttgatattc cgaaagttgt tagagaaaca attaaagaaa ttggatacac
     2221 aagagcaaaa tatggttatg attatgaaac aatggcaatt ttaactgcga ttgatgaaca
     2281 atcacctgac attgcacaag gcgtggataa agcattagag tatcgtgata aagatagcga
     2341 agaagaaatt gaagcaacag gtgcaggtga ccaaggttta atgtttggtt acgcaacgaa
     2401 tgaaacagag acgtatatgc ctctagcaat ttatttatca catcaattgg caaaacgttt
     2461 atcagatgtg cgtaaagatg gtacgttaaa ttatttacga ccagacggta aagttcaggt
     2521 cactgttgaa tatgatgaaa atgataaccc agtacgtatt gatacgattg tcgtatcaac
     2581 acaacatgct gaggatgtga cacttgaaca aattcaagaa gacattaaag cacatgtcat
     2641 ttatccaact gttccagaaa acttgattaa tgaacaaact aaattctaca tcaatcctac
     2701 aggacgcttt gtaatcggtg gacctcaagg tgatgcagga ttaacaggac gtaaaatcat
     2761 tgttgatacg tacggtggct atgcacgtca tggtggcgga tgcttcagtg gtaaggatcc
     2821 tacaaaagta gaccgttcag ctgcatacgc tgcacgttat gtggctaaaa atattgttgc
     2881 agcaggttta gcagatcaat gtgaagtaca attggcatat gcgattggtg ttgcagaacc
     2941 agtgtcaatt gcaattgata catttggaac aggtaaagtt tctgaagggc aacttgtcga
     3001 agcagttaga aaacactttg acctaagacc tgcaggtatt attaaaatgc ttgatttgaa
     3061 acaaccgatt tacaaacaaa ctgctgctta tggtcatttt ggacgtacag atgtcttatt
     3121 cccatgggaa aaattagaca aagtggaaga attaaaagac gcagtaaaat aataagatga
     3181 attgaatata gctaaataaa agattttcgg tatataaatg ataatatgta tcgaaaatct
     3241 ttagcttttc tattataatt aaagttattc atatattaaa aaggagcgtt gttgtgatgg
     3301 atttatcttc accgatagtc attggactca ttattgcagt agtcattgca ttaatctttt
     3361 ttgtcctttt tttagttgcc ttaggtagta agaaaaaagt taagcgacaa acagaagaaa
     3421 agtatgaaca acaagaacaa aacattaaaa agtctcatga agaagcttta gagaaagagc
     3481 gtattcaaaa taaaaagaca attacaaaac aacaagaaga ttataatcac atggttagta
     3541 caaaagatcg tgagattgac gcattaaaat tgttctcgaa aaatcatagt gaatatgtaa
     3601 cggacatgag attaattggt attcgtgaaa gattagttaa agaaaaacga attcgaccag
     3661 aagatatgca cattatggca aatatctttt taccaaagga tggctttaat aatattgaac
     3721 gaattagtca tttagtatta acaagaactg gtctttatat tattgattct caattgttaa
     3781 aaggtcatgt ttataatggt attagtggcg gtcaatttaa agatttacca ccaatggaac
     3841 aagtatttga tacattggat ttagataaat ctagaccgca aactattgtg atggatcaaa
     3901 atgatgacaa acgttcatta tcatttgtaa attattcaga tcaaattgag gcgattaaac
     3961 aattggctga agacttacaa aaagaattag gtgccaaata tacaccaact tcaattttat
     4021 actttaatct taaaaatgaa ggcgatgtaa cgatttcaaa ttataatcaa aatagtgctg
     4081 tcaaagtgct tgtcggtgct gaacaattag acgagttctt taacaaattt gtattccatg
     4141 gtcgtattca atacaacgtg gaagacttac aacaaatgat ggataaaatt gaatcattca
     4201 attaaatgtt aggctgaagt gatgactata tgttgttact tcggcttttt tagtttgtca
     4261 taaattaaac agaattactt ttacaatttg attaatattt gagagatatt actagaattt
     4321 tagtggttga ttgctctaag tgttttttct catgtattca actcagattt gttatgataa
     4381 cagggtataa tacatcaaaa aggtgaggcg atatttatgg aggttaaaac attttataat
     4441 ggaaacacga tgccacaaat tggtttaggg acatttcgtg tggaaaatga tgaaaattgc
     4501 atggaaagtg ttaagtatgc cattgaacaa ggatatcgta gtattgatac cgcaaaagtt
     4561 tatgggaatg aagaacaagt aggtgctggc attcgtgccg gattggagtc aactggtatt
     4621 gctagagaag acttatttat tacttcaaaa ttatattttg aggatttcgg tcgtgaaaat
     4681 gtagcagctg cttacgaagc tagtttatct agattaggtt taaaatactt agatttgtat
     4741 ctagtacatt ggccaggtac gaacgaagcc gtaatggttg atacatggaa aggtatggaa
     4801 gatttatata aaaataataa tgttaaaaat ataggtgtca gcaattttga acctgaacat
     4861 ttggaagctt tgttggcaca agtgtcaatt aaacctgtaa ttaatcaagt tgaatatcat
     4921 ccatatttaa cccaacataa attgaaatta tatttggcag cacaacgtat cgtgatggaa
     4981 tcttggtcac cattgatgaa tgcacaaatt ttaaatgatg agacaattaa agacattgct
     5041 caagaattag gaaagtcacc tgcccaagtt gttttaagat ggaatgtgca gcatgctgtg
     5101 gttacaatcc ctaaatcggt gacaccaaac agaatctctg aaaatttcca aatatttgat
     5161 ttcgaattat cagatgaaca aatgacgcga attgatggtt taaatcaaga taagagaatt
     5221 ggacctgatc caaaaaaatt tgaaggctag attaaaatcg ctcaactgat gaaaaggtta
     5281 gatgaattgt cagggcttgg gacattaa
//
LOCUS       DALAGD010000137         5252 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.140, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000137 DALAGD010000000
VERSION     DALAGD010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5252
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.140"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            81..1775
                     /gene="ezrA"
                     /locus_tag="O2701_002543"
     CDS             81..1775
                     /gene="ezrA"
                     /locus_tag="O2701_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HCZ4294409.1"
                     /translation="MVLYIILAIIVIILIAVGVLFYLRSNKRQIIEKAIERKNEIETL
                     PFDQNLAQLSKLNLKGETKTKYDAMKKDNVESTNKYLAPVEEKIHNAEALLDKFSFNA
                     SQSEIDDANELMDSYEQSYQQQLEDVNEIIALYKDNDELYDKCKVDYREMKRDVLANR
                     HQFGEAASLLETEIEKFEPRLEQYEVLKADGNYVQAHNHIAALNEQMKQLRSYMEEIP
                     ELIRETQKELPGQFQDLKYGCRDLKVEGYDLDHVKVDSTLQSLKTELSFVEPLISRLE
                     LEEANDKLANINDKLDDMYDLIEHEVKAKNDVEETKDIITDNLFKAKDMNYTLQTEIE
                     YVRENYYINESDAQSVRQFENEIQSLISVYDDILKEMSKSAVRYSEVQDNLQYLEDHV
                     TVINDKQEKLQNHLIQLREDEAEAEDNLLRVQSKKEEVYRRLLASNLTSVPERFIIMK
                     NEIDHEVRDVNEQFSERPIHVKQLKDKVSKIVIQMNTFEDEANDVLVNAVYAEKLIQY
                     GNRYRKDYSNVDKSLNEAERLFKNNRYKRAIEIAEQALESVEPGVTKHIEEEVIKQ"
     gene            complement(1849..1989)
                     /locus_tag="O2701_002544"
     CDS             complement(1849..1989)
                     /locus_tag="O2701_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294410.1"
                     /translation="MIMLFSLLILIKTTHLITFIFKQLLFIFIVPFIYPYKKEARQRI
                     VG"
     gene            2138..3286
                     /locus_tag="O2701_002545"
     CDS             2138..3286
                     /locus_tag="O2701_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCZ4294411.1"
                     /translation="MFILIYLDNAATTKAFEEVLDTYLKVNQSMYYNPNSPHKAGLQA
                     NQLLQQAKTQINAMINSKTNYDVVFTSGATESNNLALKGIAYRKFDTAKEIITSVLEH
                     PSVLEVVRYLEAHEGFKVKYVDVKKDGSINLEHFKELMSDKVGLVTCMYVNNVTGQIQ
                     PIPQMAKVIKNYPKAHFHVDAVQAFGKISMDLNNIDSISLSGHKFNGLKGQGVLLVNH
                     IQNVEPTVHGGGQEYGVRSGTVNLPNDIAMVKAMKIANENFEALNAFVTELNNDVRQF
                     LNKYHGVYINSSTSGSPFVLNISFPGVKGEVLVNAFSKYDIMISTTSACSSKRNKLNE
                     VLAAMGLSDKSIEGSIRLSFGATTTKEDIARFKEIFIIIYEEIKELLK"
     gene            3286..4509
                     /gene="thiI"
                     /locus_tag="O2701_002546"
     CDS             3286..4509
                     /gene="thiI"
                     /locus_tag="O2701_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCZ4294412.1"
                     /translation="MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVK
                     GKRDRMYIELEDHADINEITYRLSKIFGIKSISPVLKVEKTIEAISAAAIKFAQQFEE
                     NSTFKIDVKRADKNFPMDTYELQRELGGAVLKHFDNISVNVKRPDHEIRVEVRLDAIY
                     MYEEVVPGSGGLPVGTGGKTLLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQ
                     AKEKVIELTRILAERVGPIKLHIVPFTELQKQVNKVVHPRYTMTSTRRMMMRVADKLV
                     HQIGALAIVNGENLGQVASQTLHSMYAINNVTSTPVLRPLLTYDKEEIIIKSKEIGTF
                     ETSIQPFEDCCTIFTPKNPVTEPNFDKVVQYESVFDFEEMINRAVENIETLEITSDYK
                     TIKEQQTNQLINDFL"
     gene            4553..>5252
                     /locus_tag="O2701_002547"
     CDS             4553..>5252
                     /locus_tag="O2701_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HCZ4294413.1"
                     /translation="MDLNLTMIIIIILFGFIAAFIDSVVGGGGLISTPALLAIGLPPS
                     VALGTNKLASSFGSLTSTIKFIRSGKVDLYVVAKLFGFVFLASACGAYIATMVPSQIL
                     KPLIIIALSSVFIFTLLKKDWGNTRTFTQFTFKKAIIFAALFILIGFYDGFVGGGTGS
                     FMLFVLLVFGFDFLSAAGNAKVLNFASNIGALVLFMVLGQVDYVIGLIMAISMIAGSY
                     AGAHFAIKQGVGYVK"
BASE COUNT         1942 a          664 c          999 g         1647 t
ORIGIN      
        1 atggcaataa attcatttta taatttatcc ttaagcaaaa ttacgttata atagtaatga
       61 taaattagga ggagaagcat atggtgttat atatcatttt ggcaataatt gtgattatat
      121 tgattgctgt aggtgtatta ttctatttac gttcaaataa acgacaaatt attgaaaaag
      181 caatcgaacg taaaaatgaa attgagacgt taccttttga tcaaaacctt gcacaattat
      241 ctaagttgaa tttaaaaggt gaaacaaaaa cgaaatacga tgcaatgaaa aaggacaacg
      301 tagaaagtac aaataagtat ctagctcctg tggaagaaaa aatccataat gctgaggctt
      361 tattagataa atttagtttc aacgcatctc aaagtgaaat tgatgatgca aatgagttga
      421 tggatagtta cgaacaaagc tatcagcaac aattagaaga tgtaaatgaa attattgcgt
      481 tatacaaaga taatgatgaa ttatatgaca aatgtaaggt tgattatcgt gaaatgaaac
      541 gtgatgtttt agcaaatcgt catcaatttg gtgaggcagc aagtctactt gaaactgaaa
      601 ttgaaaaatt tgagccaagg ttagagcaat atgaagtact aaaagctgat ggtaattatg
      661 tacaagcgca caaccatata gctgccttga atgaacaaat gaaacagcta agatcttata
      721 tggaagaaat accagaatta attagagaaa ctcaaaaaga attacctggt caattccaag
      781 atttaaaata tggttgccgt gatcttaaag ttgaagggta tgatctggat cacgtgaaag
      841 tagacagtac attacaaagc ttaaaaacag agcttagttt cgttgaacca ttaattagcc
      901 gcttagaatt agaagaagct aatgataaac tagctaatat caatgataag ttagatgaca
      961 tgtatgattt aattgaacat gaagttaaag ctaaaaatga tgtcgaagaa acaaaagata
     1021 tcattacgga taacttattc aaagctaaag acatgaatta tacattgcaa acagaaattg
     1081 aatatgtacg tgaaaactac tatataaatg aatctgatgc tcagagtgtt cgtcaatttg
     1141 aaaatgaaat tcaaagttta atttctgtat atgatgatat tttaaaagaa atgtctaaat
     1201 ctgctgtgcg atatagcgag gttcaggata atttacaata tttagaagat catgtcacag
     1261 ttattaatga caaacaagaa aagctacaaa atcatctgat tcaattgcgt gaagatgaag
     1321 cagaagcaga agacaatctg ctacgagtac aatcgaagaa agaagaagtg tatcgtcgat
     1381 tacttgcttc taacttaaca agcgttcctg aaaggtttat catcatgaaa aatgaaattg
     1441 atcatgaagt tcgtgatgtt aacgaacaat ttagtgaacg tccaatacac gttaaacagt
     1501 taaaagataa agtgtctaaa attgtgattc aaatgaatac atttgaagat gaagcaaatg
     1561 atgttcttgt taatgctgtt tatgcagaga aattaattca atatggaaat agatatcgta
     1621 aggactatag caatgttgat aagagcttaa atgaagctga acgattattt aaaaataatc
     1681 gctataagcg tgcgattgaa attgcagagc aagctcttga aagtgttgag ccaggtgtta
     1741 ctaaacatat tgaagaagaa gttattaagc aatagaaact agtatgtagt tatacttaaa
     1801 taatatgagc actctgtcaa attggactga tgagtttaat aattgaagtt agccaacgat
     1861 acgttgtcta gcttcttttt tatatggata aatgaaaggg acaataaata taaatagcaa
     1921 ttgtttaaag ataaacgtaa tcaaatgtgt tgttttaatt aatataagta gtgaaaaaag
     1981 cataatcaca cagctgttta aatagagtga aatagtctaa ttcttattta ataagtagaa
     2041 ataagattat tttgcgctat aacctttatt ttttgtcgga taatagtata atgcttgagt
     2101 aaaacaattt taacgattga tgattaatga aagagaagtg tttattttga tatatctaga
     2161 taatgcggca acgacgaaag catttgaaga agtgttagat acttatttaa aagtaaatca
     2221 atcaatgtat tataatccga atagtccgca taaagctggt ttgcaggcaa atcaattact
     2281 acaacaagca aaaacccaaa ttaatgcaat gattaattca aaaacaaatt atgatgttgt
     2341 attcactagt ggtgcaactg aatccaataa tcttgcttta aaaggtattg cctatcgtaa
     2401 atttgataca gcgaaggaaa taattacatc cgtgttagag catccgtccg tattagaggt
     2461 tgtaagatat ttggaagcac acgaaggatt taaagttaaa tatgttgatg taaagaaaga
     2521 tggcagtatt aacttagaac acttcaaaga attaatgtca gacaaagtcg gtttagtaac
     2581 atgtatgtat gtaaataatg taactggaca aatacagcct attccacaaa tggctaaagt
     2641 tataaaaaat tatcctaagg cacattttca tgtagatgcg gttcaagcat tcggcaaaat
     2701 ttcaatggat ctcaataaca tagatagtat tagtttaagt ggacacaagt ttaatggttt
     2761 aaaaggacaa ggcgtcttac ttgtaaatca cattcaaaat gttgaaccaa ctgtccatgg
     2821 tggtggtcaa gaatatggcg ttagaagtgg aacagttaat ttgccaaatg atattgcaat
     2881 ggttaaagcg atgaagatag ctaatgaaaa ctttgaagca ttgaatgcat ttgttactga
     2941 gttaaataat gacgtccgtc aatttttaaa taaatatcat ggagtttata ttaattcttc
     3001 aacttcaggt tcaccattcg ttttaaatat tagttttcct ggcgtaaaag gtgaagtatt
     3061 agttaatgct ttttcaaaat atgacattat gatatctacg acaagtgctt gttcatctaa
     3121 acgtaataaa ttaaatgaag tattggctgc aatgggatta tcagacaaat ctattgaagg
     3181 tagtataaga ttatcatttg gggctactac aactaaagaa gatatagcga ggtttaaaga
     3241 aatatttatc atcatttatg aggaaattaa ggagttgcta aaataatgaa gtatgatcac
     3301 ttgcttgtta gatacgggga gttaacatta aagggttcaa atagaaagaa atttgtaaat
     3361 caattaagaa ataatgtaaa taagtcatta aaaggacttg atgggtttgt cgttaaaggc
     3421 aaacgagatc gtatgtatat tgaacttgaa gaccatgcag atataaatga aataacatat
     3481 cgattatcaa aaattttcgg tattaaatct attagtccag tattaaaagt agaaaaaaca
     3541 atagaggcaa taagtgcagc ggcaattaaa tttgcgcagc aatttgaaga aaacagcaca
     3601 tttaaaattg atgtgaagcg tgccgataaa aatttcccaa tggatacgta tgaattacag
     3661 cgtgaattgg gtggtgcagt attgaagcac ttcgacaata tttcagtgaa tgtcaaacgt
     3721 ccagatcatg aaattcgagt ggaagttaga ttagatgcaa tttatatgta tgaagaagtt
     3781 gttccgggtt caggtggatt accagttggt actggtggta agacgttact aatgctttca
     3841 ggcggtatag actcaccagt tgctgggatg gaagtgatga gacgtggcgt aacaattgaa
     3901 gcgattcatt tccatagtcc accatttaca agtgatcaag caaaagaaaa agttattgaa
     3961 ttgacacgta ttttagctga acgtgttgga ccaattaaat tgcatattgt accatttaca
     4021 gaattgcaaa aacaggtaaa taaagttgta catccaagat atacaatgac ttcaacgaga
     4081 cgtatgatga tgcgtgttgc tgataaatta gtacatcaaa taggggcttt agctattgta
     4141 aatggtgaaa acctagggca ggtagccagt caaacacttc atagcatgta tgcaattaat
     4201 aatgtaactt ctactcctgt attacgtcct ttattaactt acgataaaga agaaattatt
     4261 attaaatcga aagaaattgg tacatttgaa acatctattc aaccatttga agattgttgt
     4321 acaattttca cccctaaaaa tccagtaacc gaaccaaact ttgataaggt agtccaatat
     4381 gaaagtgtct ttgattttga agagatgatt aatcgtgctg ttgaaaatat tgaaacactt
     4441 gaaataacta gtgattataa aactattaaa gaacagcaaa caaaccaatt aataaacgac
     4501 tttttataaa taaaatccta gagtaaattt aaacataagg ggatgttaaa ctatggattt
     4561 gaacttaacg atgattataa tcataatttt atttggtttt atcgcggcgt ttatagattc
     4621 ggttgtaggg ggtggcggtt taatttctac gccagcatta ttagcaatcg gtctaccacc
     4681 atctgtggct ttaggtacaa ataaattggc aagttcgttt ggttctttaa ctagtacgat
     4741 aaagtttata aggtccggta aagtggactt atatgttgtt gccaaattat ttggttttgt
     4801 atttttggca tctgcatgtg gcgcatatat tgcaacgatg gttccgtcac aaatattgaa
     4861 acctttaatc atcattgcac tttcgtcggt gtttatattc acattactta aaaaagattg
     4921 gggcaataca cgcacgttta ctcaatttac atttaagaaa gccataatat ttgcagcact
     4981 ttttatatta atcggctttt atgatggatt tgtaggtggg ggaacaggtt catttatgct
     5041 ttttgtattg ttagtctttg gttttgattt tttaagtgca gcaggaaatg ctaaagtttt
     5101 gaactttgct tctaatatag gtgcgcttgt attatttatg gtattaggac aagtagatta
     5161 tgtaataggt ttaattatgg ctataagtat gattgctggg tcatatgctg gggctcattt
     5221 tgctattaaa caaggtgttg gttatgtaaa ag
//
LOCUS       DALAGD010000138         5148 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.142, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000138 DALAGD010000000
VERSION     DALAGD010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5148
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.142"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            236..1168
                     /locus_tag="O2701_002548"
     CDS             236..1168
                     /locus_tag="O2701_002548"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HCZ4294414.1"
                     /translation="MLTTEKLVETLKLDLIAGEEGLSKPIKNADISRPGLEMAGYFSH
                     YASDRIQLLGTTELSFYNLLPDKDRAGRMRKLCRPETPAIIVTRGLQPPEELVEAAKE
                     LNTPLIVAKDATTSLMSRLTTFLEHALAKTTSLHGVLVDVYGVGVLITGDSGIGKSET
                     ALELVKRGHRLVADDNVEIRQINKDELIGKPPKLIEHLLEIRGLGIINVMTLFGAGSI
                     LTEKRIRLNINLENWNKQKLYDRVGLNEETLSILDTEITKKTIPVRPGRNVAVIIEVA
                     AMNYRLNIMGINTAEEFSERLNEEIIKNSHKSEE"
     gene            1174..2013
                     /locus_tag="O2701_002549"
     CDS             1174..2013
                     /locus_tag="O2701_002549"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCZ4294415.1"
                     /translation="MGIVFNYIDPVAFNLGPLSVRWYGIIIAVGILLGYFVAQRALVK
                     AGLHKDTLVDIIFYSALFGFIAARIYFVIFQWPYYAENPSEIIKIWHGGIAIHGGLIG
                     GFIAGVIVCKVKNLNPFQIGDIVAPSIILAQGIGRWGNFMNHEAHGGSVSRAFLEQLH
                     LPNFIIENMYINGQYYHPTFLYESIWDVAGFIILVNIRKHLKLGETFFLYLTWYSIGR
                     FFIEGLRTDSLMLTSNIRVAQLVSILLILISISLIVYRRIKYNPPLYSKVGALPWPTK
                     KVK"
     gene            2021..2506
                     /locus_tag="O2701_002550"
     CDS             2021..2506
                     /locus_tag="O2701_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCZ4294416.1"
                     /translation="MRKFLSKTHHHTNPLWRVYRLVKFSKVFKNVIIIEFSKFIPSMV
                     LKRHIYKQLLNINIGNQSSIAYKVMLDIFYPELITIGSNSVIGYNVTILTHEALVDEF
                     RYGPVTIGSNTLIGANATILPGITIGDNVKVAAGTVVSKDIPDNGFAYGNPMYIKMIR
                     R"
     gene            2514..3953
                     /locus_tag="O2701_002551"
     CDS             2514..3953
                     /locus_tag="O2701_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCZ4294417.1"
                     /translation="MAQKNNNVIPMTFDDAFYRKMAKQKFKQREYKRAAEYFEKVLEL
                     SPDDMEIQIDYAQCLVQLGIAKKAEHLFYDNIIYNRHLEDSFYELSQLNIEVNEPNKA
                     FLFGINYVIVSDDQDYRDELDQMFDVKYQSEEQIELEAQLFVVQILFQYLFSQGRLKD
                     AKNYVLHQPQEVQDHRVVRNLLAMCYLYLGEYDTAKALYEALLQEDSTDIYALCHYTL
                     LLYNTKENEQYQKYLKILNKVVPMNDDESFKLGIVLSYLKQYRASQQLLYPLYKKGKF
                     LSIQMYNALAYNYYYLGEEDESHYYWDKLKQISKVEIGHAPWVIENSKEVFDQHILPL
                     LQSDDSHYRLYGIFLLDQLNGKEIVMTESIWQVLENLNNYEKLYLTYLVQGLTLNKLD
                     FIHRGLLTLYHNELFVSENDVMVAWINQGELIIAEKVDLTDVEPYIGAFIYLYFKNQP
                     RNVTKKQITTWLGITQYKLNKMIEFLLSI"
     gene            4020..4955
                     /gene="trxB"
                     /locus_tag="O2701_002552"
     CDS             4020..4955
                     /gene="trxB"
                     /locus_tag="O2701_002552"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCZ4294418.1"
                     /translation="MTEIDFDIAIIGAGPAGMTAAVYASRANLKTVMIERGIPGGQMA
                     NTEEVENFPGFEMITGPDLSTKMFEHAKKFGAVYQYGDIKSVEDKGEYKVINFGNKEL
                     TAKAVIIATGAEYKKIGVPGEQELGGRGVSYCAVCDGAFFKNKRLFVIGGGDSAVEEG
                     TFLTKFADKVTIVHRRDELRAQRILQDRAFKNDKIDFIWSHTLKSINEKDGKVGSVTL
                     TSTKDGSEETHEADGVFIYIGMKPLTAPFKDLGITNDVGYIVTKDDMTTSVPGIFAAG
                     DVRDKGLRQIVTATGDGSIAAQSAAEYIEHLNDQA"
BASE COUNT         1824 a          664 c          999 g         1661 t
ORIGIN      
        1 attccaatat atcattgtag agcttaggtc attgattttt ggctcggact tttatggcga
       61 tatgaaccat gtaaattaag caagcaataa attaatgatt gatattgact tgtaaaataa
      121 taacaataat gaacaattaa tatttatttt agcttttcaa tgtagattgg tgttatattt
      181 ttgatatgat aagaagagat gtaagagtag ggataaatac aattgaggtg aacccatgtt
      241 aacgacagaa aaactagttg aaacattaaa gttagattta atcgctggtg aagaaggact
      301 atcgaagcca attaaaaatg ctgatatatc aagaccgggc ttagagatgg caggttattt
      361 ttcacattat gcgtcagata gaatacaact attaggaaca acggaactat cgttttacaa
      421 tttattacca gataaggatc gcgcaggtcg tatgcgtaaa ctatgcagac cagaaacgcc
      481 tgcaattatt gtgacacgtg gattgcagcc accagaagaa ttagttgaag ctgcaaaaga
      541 attaaatacc ccacttatag ttgctaaaga tgcgactaca agtttaatga gtcgcttaac
      601 aacgttttta gagcatgcac ttgcaaagac gacatcttta catggtgttt tagtagatgt
      661 ttacggtgtt ggtgtactaa ttaccggtga ttcaggaata ggtaaaagtg agactgcgtt
      721 ggaattagtt aaacgtgggc atagattagt agcagatgat aatgtagaaa tacgtcaaat
      781 taataaagat gaactaatag ggaaaccacc aaagttaata gaacatctat tagaaatacg
      841 tggactaggt attatcaatg ttatgacttt atttggcgcg ggttcaatat taactgaaaa
      901 acgaattaga ttaaatatta atttggaaaa ctggaacaag caaaagttat atgaccgcgt
      961 aggtcttaat gaagagacgc taagtatttt agatactgaa atcactaaaa aaacaatacc
     1021 tgtaagacct ggtagaaatg ttgcggtaat tattgaggtc gctgcaatga actatcgatt
     1081 aaatatcatg ggcattaaca cggccgaaga atttagtgaa agattaaatg aagaaattat
     1141 caagaacagt cataagagtg aggagtaggt tgaatgggta ttgtatttaa ctatatagat
     1201 cctgtggcat ttaacttagg accactgagt gtacgatggt atggaattat cattgctgtc
     1261 ggaatattac ttggttactt tgttgcacaa cgtgcactag ttaaagcagg attacataaa
     1321 gatactttag tagatattat tttttatagt gcactatttg gatttatcgc ggcacgaatc
     1381 tattttgtga ttttccaatg gccatattac gcggaaaatc caagtgaaat tattaaaata
     1441 tggcatggtg gaatagcaat acatggtggt ttaataggtg gctttattgc tggtgttatt
     1501 gtatgtaaag tgaaaaattt aaacccattt caaattggtg atatcgttgc gccaagtata
     1561 attttagcgc aaggaattgg acgctggggt aactttatga atcacgaggc acatggtgga
     1621 tcggtgtcac gcgctttttt agaacaatta catttgccta attttataat agaaaatatg
     1681 tatattaacg gccaatatta tcatccaaca ttcttatatg aatccatttg ggatgtcgct
     1741 ggatttatta tcttagttaa tattcgtaaa catttaaaat taggagaaac attcttttta
     1801 tatttaactt ggtattcaat tggtcgattc tttatagaag gattacgtac agatagctta
     1861 atgctcacaa gtaatattag agttgcacaa ttagtatcaa ttcttttaat tttaataagt
     1921 ataagtttaa ttgtatatag aaggattaag tataatccac cgttgtatag caaagttggg
     1981 gcgcttccat ggccaacaaa aaaagtgaag tagtgatagt ttgaggaaat ttttatcaaa
     2041 aacacatcat catacaaacc ctttatggcg tgtataccgt cttgttaaat tttcgaaagt
     2101 ttttaagaat gtaattatca ttgaattttc gaaatttatt ccaagtatgg tactgaaaag
     2161 acatatatat aaacaacttt taaatattaa tatcggtaat caatcgtcga tagcttataa
     2221 agtaatgtta gatatttttt acccagaact gattacgatt ggtagtaaca gtgttattgg
     2281 ttacaatgta acaattttga cgcatgaagc attagttgat gaatttcgtt atggaccagt
     2341 gacgatagga tctaacactt tgattggtgc aaatgctacc attttacccg gtataacgat
     2401 tggtgacaat gtaaaagttg cagctggtac ggttgtttca aaagatatac cggataatgg
     2461 atttgcatat ggcaacccta tgtatataaa aatgattagg aggtgacaat tttatggcgc
     2521 aaaagaataa taatgtaatt ccaatgactt ttgatgatgc attttatcgt aaaatggcta
     2581 aacagaagtt taaacaaaga gaatataaac gagctgctga atactttgaa aaagtgttag
     2641 aattgtcacc tgatgatatg gaaattcaaa ttgattatgc acaatgtcta gtgcaacttg
     2701 gtattgctaa aaaagcagaa catttatttt atgacaatat tatttataat aggcatctag
     2761 aagatagctt ttatgaattg agtcagctca acattgaagt taacgaacca aacaaggcat
     2821 tcttgtttgg tattaattat gttattgtta gcgacgacca agattataga gatgaattag
     2881 atcaaatgtt tgatgtgaaa tatcaaagtg aagaacaaat tgaacttgaa gctcaattgt
     2941 ttgtagttca aatactattc caatatcttt tttctcaagg tcgattaaaa gatgcaaaga
     3001 attatgtctt acatcaacca caagaagttc aagatcatcg tgtagtacgt aatttattgg
     3061 caatgtgtta tttatatctc ggtgaatatg atacggctaa agcattgtac gaagcactat
     3121 tacaagagga tagtacagat atatatgcat tatgccatta tactttgcta ctttataaca
     3181 ctaaggaaaa tgaacaatat caaaaatatt taaaaatatt aaacaaagtt gtacctatga
     3241 atgacgatga aagttttaaa ttaggtattg tattaagtta tttaaagcag tatcgtgcat
     3301 cacaacaatt gttgtaccct ttatataaaa aagggaaatt tttatcaatt caaatgtaca
     3361 atgctttagc atataattat tattatttag gtgaagaaga cgaaagtcat tactactggg
     3421 ataaattgaa gcaaatttct aaagtggaaa ttggacatgc gccttgggta attgaaaata
     3481 gcaaagaagt ttttgaccaa catattttgc cattacttca aagtgatgac agtcattatc
     3541 gtttatatgg tattttttta ttggatcaat taaatggtaa agaaattgtg atgacggaaa
     3601 gtatttggca ggttttggaa aatctaaata attatgagaa attgtattta acgtatttag
     3661 ttcaaggttt aacgctcaat aaattagact tcattcatcg cggcttatta acgctttacc
     3721 ataatgaatt atttgtaagt gaaaatgatg taatggttgc atggattaat caaggtgaac
     3781 tcataattgc tgaaaaagta gatttaactg atgttgagcc atatatcggt gcgtttattt
     3841 atttgtattt taaaaatcaa cctcgaaacg ttacaaagaa gcaaattaca acatggttag
     3901 gcataacaca atataaactg aacaaaatga ttgaatttct cttgagcata tagatttatg
     3961 aaaagttaga tttattatat aatgcgcata atgattaata atgaggaggc gttaataaaa
     4021 tgactgaaat agattttgat atagcaatta tcggtgcagg tccagctggt atgactgctg
     4081 cagtatacgc atcacgtgct aatttaaaaa cagttatgat tgaaagaggt attccaggcg
     4141 gtcaaatggc taatacagaa gaagtagaga acttccctgg tttcgaaatg attacaggtc
     4201 cagatttatc tacaaaaatg tttgaacacg ctaaaaagtt tggtgcagtt tatcaatatg
     4261 gagatattaa atctgtagaa gataaaggcg aatataaagt gattaacttt ggtaataaag
     4321 aattaacagc gaaagcggtt attattgcta caggtgcaga atacaagaaa attggtgttc
     4381 cgggtgaaca agaacttggt ggacgcggtg taagttattg tgcagtatgt gatggtgcat
     4441 tctttaaaaa taaacgccta ttcgttatcg gtggtggtga ttcagcagta gaagagggaa
     4501 cattcttaac taaatttgct gacaaagtaa caatcgttca ccgtcgtgat gagttacgtg
     4561 cacagcgtat tttacaagat agagcattca aaaatgataa aatcgacttt atttggagtc
     4621 atactttgaa atcaattaat gaaaaagacg gcaaagtggg ttctgtgaca ttaacgtcta
     4681 caaaagatgg ttcagaagaa acacacgagg ctgatggtgt attcatctat attggtatga
     4741 aaccattaac agcgccattt aaagacttag gtattacaaa tgatgttggt tatattgtaa
     4801 caaaagatga tatgacaaca tcagtaccag gtatttttgc agcaggagat gttcgcgaca
     4861 aaggtttacg ccaaattgtc actgctactg gcgatggtag tattgcagcg caaagtgcag
     4921 cggaatatat tgaacattta aacgatcaag cttaattcga agtcgaatta agatgttgag
     4981 ctgtaaatta tttggatatt tattttaata gtgtcatcac agcgttaaaa taatgtctta
     5041 cttttaaatt aaagcaaatt atatagaaaa ctagaactta gtacgtatca tttgtgcgtt
     5101 tcaatgagtt ctagtttttt tatatgttat attaaactta taacttta
//
LOCUS       DALAGD010000139         5093 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.143, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000139 DALAGD010000000
VERSION     DALAGD010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.143"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(124..963)
                     /gene="noc"
                     /locus_tag="O2701_002553"
     CDS             complement(124..963)
                     /gene="noc"
                     /locus_tag="O2701_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HCZ4294419.1"
                     /translation="MKKPFSKLFGLKNKDDIIGHIEEDRNSNVESIQIERIVPNRYQP
                     RQVFEPNKIKELAESIHEHGLLQPIVVRPIEEDMFEIIAGERRFRAIQSLNLPQADVI
                     IRDMDDEETAVVALIENIQRENLSVVEEAEAYKKLLEIGDTTQSELAKSLGKSQSFIA
                     NKLRLLKLAPKVLLRLREGKITERHARAVLSLSDSEQEALIEQVIAQKLNVKQTEDRV
                     RQKTGPEKVKAQNLRFAQDVTQARDEVGKSIQAIQQTGLHVEHKDKDHEDYYEIKIRI
                     YKR"
     gene            complement(1006..1725)
                     /gene="rsmG"
                     /locus_tag="O2701_002554"
     CDS             complement(1006..1725)
                     /gene="rsmG"
                     /locus_tag="O2701_002554"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCZ4294420.1"
                     /translation="MTVEWLAEQLKEHNIQLTETQKQQFQTYYRLLVEWNEKMNLTSI
                     TDEHDVYLKHFYDSIAPSFYFDFNQPISICDVGAGAGFPSIPLKIMFPQLKVTIVDSL
                     NKRIQFLNHLASELQLQDVSFIHDRAETFGKGVYRESYDVVTARAVARLSVLSELCLP
                     LVKKGGQFVALKSSKGEEELEEAKFAISVLGGNVTETHTFELPEDAGERQMFIIDKKR
                     QTPKKYPRKPGTPNKTPLLEK"
     gene            complement(1725..3602)
                     /gene="mnmG"
                     /locus_tag="O2701_002555"
     CDS             complement(1725..3602)
                     /gene="mnmG"
                     /locus_tag="O2701_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCZ4294421.1"
                     /translation="MVQEYDVIVIGAGHAGVEAGLASARRGAKTLMLTINLDNIAFMP
                     CNPSVGGPAKGIVVREIDALGGQMAKTIDKTHIQMRMLNTGKGPAVRALRAQADKVLY
                     QQEMKRVIEDEENLHIMQGMVDELIIEDNEVKGVRTNIGTEYLSKAVIITTGTFLRGE
                     IILGNMKYSSGPNHQLPSITLSDNLRELGFDIVRFKTGTPPRVNSKTIDYSKTEIQPG
                     DDVGRAFSFETTEYILDQLPCWLTYTNAETHKVIDDNLHLSAMYSGMIKGTGPRYCPS
                     IEDKFVRFNDKPRHQLFLEPEGRNTNEVYVQGLSTSLPEHVQRQMLETIPGLEKADMM
                     RAGYAIEYDAIVPTQLWPTLETKMIKNLYTAGQINGTSGYEEAAGQGLMAGINAAGKV
                     LNTGEKILSRSDAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTDMGY
                     ELGMISEERYARFNEKRQQIDAEIKRLSDIRIKPNEHTQAIIEQHGGSRLKDGILAID
                     LLRRPEMTYDIILELLEEEHQLNADVEEQVEIQTKYEGYINKSLQQVEKVKRMEEKKI
                     PEDLDYSKIDSLATEAREKLSEVKPLNIAQASRISGVNPADISILLIYLEQGKLQRVS
                     D"
     gene            complement(3669..5048)
                     /gene="mnmE"
                     /locus_tag="O2701_002556"
     CDS             complement(3669..5048)
                     /gene="mnmE"
                     /locus_tag="O2701_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCZ4294422.1"
                     /translation="MDLDTITSISTPMGEGAIGIVRLSGPQAVEIADKLYKGKHLLND
                     VPSHTINYGHIIDPESKEVIEEVMVSVLRAPKTFTREDIIEINCHGGILTINRVLELT
                     MTYGARMAEPGEFTKRAFLNGRIDLSQAEAVMDFIRSKTDRASKVAMNQIEGRLSDLI
                     KKQRQSILEILAQVEVNIDYPEYDDVEDATTEFLLEQSKEIKQEINRLLDTGAQGKIM
                     REGLSTVIVGKPNVGKSSMLNNLIQDNKAIVTEVAGTTRDVLEEYVNVRGVPLRLVDT
                     AGIRETEDIVEKIGVERSRKALSQADLILFVLNNNEALTQEDYTLYEVVKNEDVIVIV
                     NKMDLEQNIDINEVKDMIGDTPLIQTSMLKQEGIDELEIQIRDLFFGGEVQNQDMTYV
                     SNSRHISLLKQARQTIQDAIDAAESGVPMDMVQIDLTRTWEILGEIIGETASDELIDQ
                     LFSQFCLGK"
BASE COUNT         1469 a         1046 c          710 g         1868 t
ORIGIN      
        1 ccagcctgat caacatataa atataaattt ttatgtttca cgtaaaacaa aaaaaagaca
       61 aagctgttat gatcttagct ttgtctttta tgtgttagtc atcatgtata cgacatccta
      121 ctactaacgt ttatatattc gaatttttat ttcataataa tcttcatgat ctttgtcttt
      181 atgctcaaca tgtaatcctg tttgttgaat cgcttggata cttttgccta cctcatctcg
      241 tgcttgagtg acatcttgtg caaagcgaag gttttgtgct ttgacttttt cgggccccgt
      301 tttttggcgt actctatctt cagtctgttt cacatttagc ttttgtgcaa tgacttgctc
      361 aatcaacgct tcttgttcgc tatcagacaa tgataatacc gctctcgcat gacgttcagt
      421 aattttacct tctcttaagc gaagtagtac tttcggcgcc aacttcaata aacgcaactt
      481 atttgcaata aagctttgac ttttacctaa actttttgcc aattcacttt gcgttgtatc
      541 accaatttcc aataatttct tataggcttc cgcttcttca acaacagaca aattttctct
      601 ttgaatattc tcaattaatg caacaacagc cgtctcttca tcatccatat cacgaataat
      661 aacgtctgct tgaggtaaat ttagtgattg tattgctcta aatcggcgct ctccagcaat
      721 aatttcaaac atatcttctt caatcggtct tacaacaata ggttgtagta aaccatgttc
      781 atgtattgat tcagcaagtt ctttaatttt atttggttca aacacctgtc ttggttgata
      841 acggttggga acgatacgtt caatttgaat ggattcaaca ttactattgc gatcttcttc
      901 aatatgtcca atgatgtcat ctttgttttt caaaccaaat aattttgaaa aaggtttttt
      961 cattatccat tcgctccttt tatgttagtt gtaaaggatt atgcattatt tttcaagtaa
     1021 aggagtctta ttaggcgtcc ctggttttct tggatacttt ttcggcgtct gtcttttttt
     1081 atcaataatg aacatctggc gctctccagc atcttctggc aattcaaagg tatgtgtttc
     1141 tgtaacatta ccacctaaca cactaattgc aaattttgct tcttctaatt cttcttcacc
     1201 ttttgaagat tttaatgcaa caaactgtcc acctttttta actagcggta aacacaattc
     1261 acttaacacg gataatctag ctactgctct tgcagtaaca acatcataag actccctgta
     1321 gacaccctta ccaaatgttt ctgctctatc gtgtataaag ctgacatcct gtaattgtaa
     1381 ttctgacgct aaatggttta aaaattgaat acgcttattt aatgaatcaa caatcgtcac
     1441 ttttaactgc ggaaacatta tttttaacgg aatacttgga aaaccagctc cagcgcctac
     1501 atcacatata cttataggct gattaaaatc aaaataaaaa ctaggtgcaa tggaatcata
     1561 aaaatgtttc aaatatacat cgtgttcatc tgtaatactt gtcaaattca tcttttcatt
     1621 ccattcaaca agtaaacgat aatatgtttg aaactgttgt ttttgagtct cagttaattg
     1681 aatattatgt tcttttaatt gttctgctaa ccattctaca gtcattagtc actcaccctt
     1741 tggagtttac cttgttctaa gtaaatcaat aatatagata tgtctgctgg atttacccct
     1801 gatattctag aagcttgtgc aatatttaaa ggttttactt ctgataattt ttctcgcgct
     1861 tcagtcgcca aactatcaat cttactataa tctaagtctt ctggaatttt cttctcttcc
     1921 atacgcttaa ctttctcaac ttgttgtagt gatttattga tataaccttc atattttgtt
     1981 tgtatttcta cttgttcttc aacatctgca ttcaattgat gttcttcttc taaaagttct
     2041 aaaattatat cgtaagtcat ttcaggtctg cgtaataaat cgatagctaa aataccatct
     2101 tttaagcgag aaccaccatg ttgttcaata atcgcttgcg tatgttcgtt tggtttaata
     2161 cgaatatctg ataaacgctt aatttccgca tcaatttgct gacgtttttc attaaaacgt
     2221 gcatatcttt cttcagaaat cataccaagt tcatatccca tatccgtcaa tctcaaatca
     2281 gcattatcat gacgtagtaa caaacgatat tctgcacgtg atgttagtaa acgataaggt
     2341 tcattagtac ctttagttac aagatcatcg attaagacac caatatatgc atctgaacga
     2401 cttaatatct tttcgcctgt gtttaacact ttacctgcag cgttaatacc tgccatcaat
     2461 ccttgtcctg ctgcttcttc ataaccagat gtaccattaa tttgacctgc agtatataag
     2521 tttttaatca ttttcgtttc aagtgtaggc cataactgcg ttggcacaat cgcatcatat
     2581 tcaattgcgt agccggcacg catcatatct gctttttcaa gacctggtat cgtctctaac
     2641 atttgacgtt gcacatgttc aggaagactt gtagacaatc cttgcacata tacttcattt
     2701 gtattacgac cttcaggctc taagaaaagt tgatgtcgcg gcttatcatt aaatcgaaca
     2761 aatttatctt caattgaagg gcaataacgt ggcccggttc ctttaatcat ccctgaatac
     2821 attgcagata gatgtaaatt atcatcgata actttgtgtg tttcagcatt agtatacgtt
     2881 agccaacatg gcaattgatc taatatatat tctgttgttt caaagctgaa tgcacgacct
     2941 acatcgtcac ctggttgtat ttcagtcttc gaatagtcaa ttgtttttga atttacacgc
     3001 ggtggtgtac ctgttttaaa acgaacaata tcaaaaccaa gttctcttaa attgtctgat
     3061 aatgtgattg atggtaattg gtgatttggt ccacttgaat acttcatatt acctaaaatg
     3121 atttcaccac gtaaaaatgt tcccgttgta ataattactg ctttagataa atactctgta
     3181 ccaatatttg tacgtacacc tttaacttca ttatcttcta taataagttc gtctaccata
     3241 ccttgcatta tatgcaaatt ttcttcatct tcaatcacgc gtttcatttc ttgttgataa
     3301 agtactttat ctgcttgcgc tcttagtgct cttacagcag gtcctttacc tgtatttaac
     3361 attctcattt gaatgtgtgt tttatcgatt gtttttgcca tttgtccacc taaagcatca
     3421 atttcacgaa caacgatacc tttagctggt ccacctacag atgggttaca tggcataaat
     3481 gcaatattat ctaaatttat tgttagcatt aatgttttag caccacgtct tgcagatgct
     3541 aaacctgctt ctacacctgc atgtcccgca cctataacga ttacatcata ttcttgaacc
     3601 acaatataaa cctccttatt tgatatctta ctagccttct taagacggta ttccgtctat
     3661 ttcaattact atttacctaa gcagaattga ctgaataact gatcgatgag ttcatcactt
     3721 gcagtctcac caataatttc tcctaatatt tcccaagttc tagttaaatc aatttgtacc
     3781 atatccatag gcacaccaga ttctgctgca tcaatcgcat cttgtatcgt ttgtcttgct
     3841 tgttttaata atgaaatatg tcttgaatta gaaacataag tcatatcttg attttgtact
     3901 tctccaccaa agaacaaatc tcgaatttgt atttctaatt catcaatacc ttcttgtttt
     3961 aacattgaag tttgaattaa tggcgtatca cctatcatat ctttaacttc attaatatct
     4021 atgttttgct ctaaatccat tttattaaca attacgatta catcttcatt tttaaccact
     4081 tcatataatg tgtaatcttc ttgagtcaat gcttcgttat tgtttaatac aaataaaatt
     4141 aagtctgctt ggctaagagc ctttctagag cgttcaacac caatcttctc tactatatct
     4201 tctgtctcac gtataccagc agtatcaact aatcttaatg gcacgccacg aacattgacg
     4261 tactcttcta agacatctct agtagtacct gctacctcag ttacaatcgc tttattatct
     4321 tgtattaaat tatttaacat cgatgattta cctacgtttg gtttaccaac aataactgta
     4381 gataaacctt cacgcataat tttaccctgc gcaccggtat ctaataaacg attaatttcc
     4441 tgtttgattt ctttagactg ctctaaaaga aattcagtag tcgcatcttc aacatcatcg
     4501 tattcaggat aatcaatatt cacttccact tgagcgagta tctctaatat agattgacgt
     4561 tgttttttga ttaagtcact tagacgacct tcaatttgat tcatcgcaac tttagaagct
     4621 ctatctgtct tcgagcgaat aaagtccata actgcttcag cttgagataa atcaatacga
     4681 ccatttaaaa aggcacgttt tgtaaattca cctggctcag ccattctagc gccatatgtc
     4741 atagtaagtt ccagcactct attaatcgtt aaaataccac catgacaatt aatttctata
     4801 atatcttcgc gtgtaaatgt ttttggcgct cttaacacag acaccataac ttcttcaatc
     4861 acttctttag actctggatc aataatatga ccgtaattaa tcgtatgtga tggaacatca
     4921 tttaaaagat gttttccttt atataatttg tcagcaattt caacggcttg cggtccagac
     4981 aatcgaacaa ttccaattgc cccttcaccc attggtgttg aaatactcgt aattgtatct
     5041 aaatccatat tgctactcgc ctccttcaac gatgtgaata cattttaaag taa
//
LOCUS       DALAGD010000140         5004 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.144, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000140 DALAGD010000000
VERSION     DALAGD010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5004
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.144"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            248..1657
                     /locus_tag="O2701_002557"
     CDS             248..1657
                     /locus_tag="O2701_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCZ4294423.1"
                     /translation="MSIDMYLDRSRNQASSVGNLSQTMNSNYDALEKAITQFINDDAL
                     KGKAYTSAKQFFSTVLIPLSTSMKTLSDLTKQACDNFVSRYTSEVDSISLKESELEED
                     IRSLSQQITRYENLNNNLKKHASDNQQAISSNQQIIRTLGQQKHELEEKLRKLREFNQ
                     KSPEIFKEVEEFQKIVQQGLTQAQNFWNFSTNQFNIPSGKELDWAKASHEKYLKVAMG
                     KIEHKAEKETLNKADFAVIKAYAKEHPEDDIPKSILKYINDNKDSIKRDIGLDITSTL
                     LEQGGINASKFGVFINTAGGVKGPAGPNSFVEVKRTSGNVFIENGSKFAKGGKYLGKG
                     VAGVGFGIGMYDDLANDDKTFGEALSHNGMTLAAGSAGTAVGAGLATFVLGSNPVGWV
                     ILAGLAMSTVFALGTDLIYQNNIFGLKDKVDWVGHKIDNSIDVVKKTTEKSMDSVGNA
                     VSEAKNIISNHINPMKWAW"
     gene            1661..2287
                     /locus_tag="O2701_002558"
     CDS             1661..2287
                     /locus_tag="O2701_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCZ4294424.1"
                     /translation="MLFNIKVINKNPRYKVVQYNDEYLLIDLVSTWLVYFFPFINWFI
                     PKRYAKLSEKELENLNVDKQNKNNIFWPVTGSSFLFVVILRKYVHTFEVQLDNKILIS
                     LCFIGFIGIAAFYIYLNKKLKLKIYDDNLDNENRVILVPTFKDGSFIVFTYLLLGGCS
                     ILFLIWLMTIKPQNLLVFIMWIIITIFFFLISMGSISNKKVYAKLKKQ"
     gene            2413..3054
                     /locus_tag="O2701_002559"
     CDS             2413..3054
                     /locus_tag="O2701_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HCZ4294425.1"
                     /translation="MEILLCEVRVVNKNPRYRIIKYKNDYLMIDLVSTWLVLFFPFIN
                     WLIPKKYVQISREDFDNLNIVKPVKNKALWPAIGSILLFGTMFRDKIYIPDSHLEKNC
                     VIIICSVLLLSILVFYIYLNQKVKLSIYNDRSSNGKIMIFPSFKNLCFVLFSYFFCGG
                     LSIMFLDVLISLSIQNIIVFIAWVIMTMLFFFINMSSIIDKKIHVIYLRSYKY"
     gene            3142..3759
                     /locus_tag="O2701_002560"
     CDS             3142..3759
                     /locus_tag="O2701_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCZ4294426.1"
                     /translation="MLCETEIINKNPKYRVIKYDDEYLMVDVIRTWLVYFFPFINWFI
                     PKRCAKISREEYEKLNTVKPVKNKNFWPVVGGTILLGATSRKYIHLLNIQLEKRSVIF
                     ICFVVFLCILIFFVLLNRKLKLKVFDNKKEEQKIILVPTLKNAVLILYGYLLIGGMSI
                     LALSMLLTLENQNLITFIAWGMGLMLFFLMNITLIVNKTVKVIKR"
     gene            3834..4463
                     /locus_tag="O2701_002561"
     CDS             3834..4463
                     /locus_tag="O2701_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HCZ4294427.1"
                     /translation="MLCESRVINQNPKYRIIKYNNEYFMVDLVSTWIAYFLPMINWFI
                     PKKYAKISREEFESLNIVKPAKNNTFWPVAGFAVLLTTLTRKYIYLLNIHLEKEIVIL
                     TCCMILLGVFALFIYINTKLKLHIFDKNKSNNEKIILIPTFKNICLSLFAYILFGGLS
                     TMALSMLVTSSPQNIIEFLALIGMTACFFLLNMSSVLDKKIHVILKTNK"
     gene            4520..4636
                     /locus_tag="O2701_002562"
     CDS             4520..4636
                     /locus_tag="O2701_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294428.1"
                     /translation="MILNRGAILEQGNKTAKVIKSELITNYIMLATLQIKNN"
     gene            4659..>5004
                     /locus_tag="O2701_002563"
     CDS             4659..>5004
                     /locus_tag="O2701_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCZ4294429.1"
                     /translation="MLCESKIINKNPKYRIIKYNDEYLMIDIISTWISLFFPFINWFI
                     PKRYVKISREEFENLNIVKPAKKNVFWPVAGISTLFAVTLRKYTHLLDTQLDRKLVIA
                     ICCITFIGILTFY"
BASE COUNT         1869 a          584 c          843 g         1708 t
ORIGIN      
        1 cgcaataaaa ttaaaatatt gaaagataat ttttattgat taattgcgaa tatacaaaga
       61 caccattata tccatatgct aaaataagaa aaaaggcgac atgttatctc aatttacctg
      121 catctagact cctttacata agaaataatc atcgccgagt tttaaaattt tacaactttg
      181 tagatgtaaa aagcatttga ttttaaatag tttgaaaatc atatattttt gaaaaaggag
      241 catgccgatg agtattgaca tgtatttaga cagatctcga aaccaagctt caagtgtggg
      301 gaatttgagt caaacaatga attcaaatta tgatgcgttg gaaaaagcaa ttactcaatt
      361 tattaatgat gatgcgctta aagggaaagc gtatacgtca gctaagcaat tttttagtac
      421 ggtgttaatt ccattatcaa caagtatgaa aacattgagt gatttaacga agcaagcttg
      481 cgataatttt gtgtcacgtt atacgagtga ggttgatagc atatctttaa aagaatcaga
      541 gcttgaagaa gatatcagat cattaagtca acaaattacg cgatatgaaa atttgaataa
      601 caatttgaaa aagcatgctt ccgataatca gcaagccatt tcatcgaacc aacaaataat
      661 acgaacatta ggtcaacaaa aacatgaatt agaagagaag ctacgcaaat tgcgtgagtt
      721 taatcaaaaa tcaccagaaa tatttaaaga agttgaagaa tttcaaaaaa ttgtccaaca
      781 aggacttacc caagcgcaga atttttggaa cttttcaaca aatcaattta atattccttc
      841 aggtaaagaa cttgattggg ccaaagcaag tcatgaaaaa tatttgaaag ttgctatggg
      901 gaaaattgaa cataaagcag agaaagaaac tttaaataaa gcagactttg ctgttataaa
      961 ggcatatgcc aaagaacatc cagaagacga tatcccgaaa agtatattga aatatataaa
     1021 tgacaataaa gacagtatta aaagagatat aggattagat attacgtcaa cacttttaga
     1081 gcaaggcggt ataaatgcaa gtaaattcgg tgtatttatc aatacagcag gtggagtgaa
     1141 aggcccagca ggtccaaatt catttgtgga agtcaaacgt acatcaggta atgtgtttat
     1201 agaaaatggt agtaaatttg caaaaggcgg aaaataccta ggtaaaggtg ttgctggtgt
     1261 aggatttggt ataggtatgt atgatgacct tgcaaatgat gataaaacat ttggagaggc
     1321 gttgtcgcat aatggtatga cgcttgcagc tggatctgca gggacagcag ttggagctgg
     1381 attagcgact tttgttttag gaagtaatcc agtaggatgg gtgattttag caggtttggc
     1441 tatgagtaca gtatttgcat taggaacaga tttaatttat caaaataata tttttggatt
     1501 aaaagataaa gtagactggg tagggcataa aatcgataat agtatagatg ttgttaaaaa
     1561 aactacagaa aaatccatgg atagtgttgg gaatgctgtt agtgaagcta aaaatattat
     1621 tagcaatcat ataaatccaa tgaaatgggc gtggtgagtt ttgcttttta atattaaggt
     1681 tattaataaa aatccgagat ataaagtcgt tcaatataat gatgagtatt tgttaattga
     1741 tttagtaagc acttggcttg tatacttttt tccttttatt aattggttca ttcccaaaag
     1801 gtatgcaaaa cttagtgaga aggaacttga aaatttaaat gttgataaac aaaataaaaa
     1861 taatattttc tggccagtta ctggtagttc gtttttattt gtagttattt tacggaaata
     1921 tgtacatacg tttgaagttc aattagataa taagatttta atatctttat gttttatagg
     1981 atttataggt attgcagcat tttacattta cttaaataaa aagcttaaac taaagatata
     2041 tgatgataat ctagataatg aaaatagggt tatattagtg cctactttta aagatggaag
     2101 ttttatagtt ttcacatatc tattattagg aggttgttct atattatttt taatatggct
     2161 aatgactata aaacctcaaa atctactggt atttattatg tggattatta ttacaatttt
     2221 tttctttcta ataagcatgg gctcaattag taataaaaag gtttatgcca aacttaaaaa
     2281 gcaatagaaa ttactaaact tagattgtag ttcgtaagtt aagtaataaa caggaaaata
     2341 gtatgttact aaaatacaaa acacttataa agtatctgaa agtgtattag atataaaacc
     2401 aacgagacgg aagtggagat attgctttgc gaggttagag ttgtaaacaa aaatcccaga
     2461 tatagaatta ttaaatataa aaacgactat ctgatgattg atttagtaag cacctggtta
     2521 gttttgttct ttccgtttat taattggtta ataccaaaaa aatatgtcca aatcagcaga
     2581 gaagattttg ataatttaaa tattgtcaaa ccagttaaaa ataaagcttt atggccagct
     2641 ataggtagca tacttttatt tggcactatg tttagagata aaatatatat acctgattct
     2701 catttagaaa aaaattgcgt tattatcatt tgttcggttt tattattaag tattctagtg
     2761 ttttatatat atttaaatca gaaagtaaag ttatctatct ataatgatcg gagtagcaat
     2821 ggaaaaatta tgatttttcc atcattcaag aatctttgct ttgtactatt ttcttatttt
     2881 ttctgtggtg gattatcaat catgttctta gatgttttga ttagtttatc tatccaaaat
     2941 attatagtat ttattgcttg ggttattatg acaatgctat ttttctttat aaatatgtct
     3001 tcaataatag acaaaaagat tcatgttata tatttaaggt catataaata ttaattttga
     3061 tacaaaaggg cacaagtgtt ttgagaaaca gaaacgataa tatagctgtg tgaatttgtt
     3121 attaatagga agtgataatt attgctttgt gaaaccgaaa ttatcaataa aaatccgaaa
     3181 tacagagtta ttaagtatga tgatgagtat ttaatggtcg atgtaataag aacttggctt
     3241 gtatactttt ttccatttat taattggttc attccaaaaa ggtgcgccaa aattagtaga
     3301 gaagagtatg aaaaactgaa cactgttaag ccagttaaaa ataaaaattt ttggcctgtt
     3361 gtaggaggaa caatcctact aggtgctact tccagaaagt acatacactt acttaatatt
     3421 caattagaaa aaagatcggt tatatttata tgcttcgttg tatttctatg tattttaatc
     3481 ttttttgtgc ttctgaatcg aaaattaaaa ttgaaagttt tcgataataa aaaagaagaa
     3541 caaaaaataa ttttagtacc aacattaaaa aatgctgttc ttatattata tggctatttg
     3601 ttgattggag gcatgtcaat attagcttta agcatgttgc tgacattaga aaaccagaat
     3661 ttaataactt ttatagcttg gggtatgggg ttaatgttgt ttttcttgat gaatataaca
     3721 ctcattgtca ataaaacagt caaagttata aaaaggtgaa taaataacta attatattgt
     3781 attggccgtt cttcaaataa aaaataattt gaataaggtg gaagtgataa acagtgcttt
     3841 gcgaatctag agtcattaat caaaacccta aatatagaat tattaaatac aataatgaat
     3901 atttcatggt tgatttagta agtacttgga ttgcttattt tttgcccatg attaattggt
     3961 ttattcccaa aaagtacgcg aaaattagta gagaagaatt tgaaagttta aatattgtca
     4021 aacccgctaa aaataatact ttctggcctg ttgcaggatt tgcagtgtta ttaacaacct
     4081 taacaagaaa atatatctat ttgcttaaca tccatttaga aaaagaaata gttatattaa
     4141 catgctgtat gatacttcta ggtgttttcg cattgtttat atatataaat acaaaattga
     4201 agttacatat ttttgataaa aataaaagta ataacgaaaa gatcatatta atacctacat
     4261 ttaaaaatat ttgtttatcc ttatttgctt atatattatt tggtggattg tcaacaatgg
     4321 ctctgagtat gttagtaact tcatcccctc aaaatataat agaatttctt gctttaattg
     4381 gcatgactgc atgcttcttt ctactgaata tgtcatcggt tctagataaa aaaattcatg
     4441 ttattttaaa aacaaataag tagtaaaatt gattaactta ggtagtatcg gatacttaaa
     4501 tgttggttca taaaaagcaa tgattttaaa tcgaggagct atcttagaac agggaaataa
     4561 aacagccaaa gttataaaaa gtgaattaat aactaattat attatgttag ccacgcttca
     4621 aataaaaaat aattagaata aggtgggatt gataatcaat gctatgcgaa tctaaaatca
     4681 tcaataaaaa ccccaaatat agaattatta aatataatga tgagtactta atgatcgata
     4741 taataagcac ttggattagt ttattttttc cttttattaa ttggttcatc cccaaaagat
     4801 acgtcaaaat tagtagagaa gagtttgaaa atttaaatat tgtgaaacct gctaaaaaga
     4861 atgttttttg gccagttgca ggtatctcta ctttattcgc agttacatta agaaagtata
     4921 cacatttact tgacactcaa cttgatagaa aattagttat tgctatatgt tgtatcacat
     4981 ttatagggat tttaacattt tatg
//
LOCUS       DALAGD010000141         4652 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.146, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000141 DALAGD010000000
VERSION     DALAGD010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.146"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(10..762)
                     /locus_tag="O2701_002564"
     CDS             complement(10..762)
                     /locus_tag="O2701_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HCZ4294430.1"
                     /translation="MQALQTNSNIGEMFNIQEKENGEIAISGRELHQALEVKTAYKDW
                     FPRMLKYGFEENTDYTAIAQKRATAQGNMTHYIDHALTLDTAKEIAMIQRSEPGKRAR
                     QYFIQVEKAWNSPEMIMQRALKIANNTINQLETKIERDKPKIVFADAVATTKTSILVG
                     ELAKIIKQNGVNIGQRRLFEWLRQNGFLIKRKGVDYNMPTQYSMERELFEIKETSITH
                     SDGHTSISKTPKVTGKGQQYFVNKFLGEKQTS"
     gene            813..1142
                     /locus_tag="O2701_002565"
     CDS             813..1142
                     /locus_tag="O2701_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294431.1"
                     /translation="MTNEAKFVLLQLYSIYLDRIDEGMSKRSASYFGSDESSFNAFFL
                     DFNFEDYIDAVLELKHRDFVIASAEDGGFLEMALSREGIAYSESESKKDYKTLMDLIR
                     DLKKLII"
     gene            complement(1131..1346)
                     /locus_tag="O2701_002566"
     CDS             complement(1131..1346)
                     /locus_tag="O2701_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HCZ4294432.1"
                     /translation="MNIQEATKLAMEKGISIRRENQDVYGILPTNLQRYQCLVVSRHY
                     KKKRQTAAGRWQPSADDLIADDWILDY"
     gene            complement(1362..1625)
                     /locus_tag="O2701_002567"
     CDS             complement(1362..1625)
                     /locus_tag="O2701_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ4294433.1"
                     /translation="MKTLKELRTDYGLTQKELGDLFKVSSRTIQNMEKDSTNIKDSLL
                     SKYMSAFNVKYDDIFLGNEYENFVFTNDKKKSIILAFKEKQTS"
     gene            complement(1622..1795)
                     /locus_tag="O2701_002568"
     CDS             complement(1622..1795)
                     /locus_tag="O2701_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCZ4294434.1"
                     /translation="MFLAIIIFLSLFILLTSFFLIIRNFHIIVKFFTKKTYLMLTIRK
                     FRIILGLRKAVTT"
     gene            1758..2471
                     /locus_tag="O2701_002569"
     CDS             1758..2471
                     /locus_tag="O2701_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ4294435.1"
                     /translation="MNKERNIIIAKNIRKFLNDSNMSQKKLAELINIKPSTLSDYLNL
                     RSNPSHGVIQRIADVFEVGKSDIDTTYKDDNDITSIYNKLTPPRQENVLNYANEQLEE
                     QNSKGDNVVDINSYKQEKTPVNVNGCVSAGVGERLHDETLFTEMVKGPIPTHDLALKV
                     NGDSMEPMFKDGEIIFVEKTHNIKNGQIGIFIIEEEAYVKKVFVEDDRLTLVSLNKDY
                     DDLHFYRNESVRLIGKVIL"
     gene            2487..3419
                     /locus_tag="O2701_002570"
     CDS             2487..3419
                     /locus_tag="O2701_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCZ4294436.1"
                     /translation="MKKYDIAVLDFETMNEHMNSPCEVAVSLIKDLSIVKVYSSYINP
                     PNNRYNLKNAKIHKIPEDVILKAPKYPDIYQEILYLLKESHLIIAHNALFDISVLKNT
                     NNYYDLPVPNFMYVDSINIFRSFHAISSFKLENLCSLYDIDKEKLHSAKFDVLALSKM
                     LISLAKNNQHYSVLKLIHYMPKQYIRFSKYSNSPTKLFDSGFQKIHMKISEINKIEVE
                     SVIPILKDKNVVFTGNFDTEKQDLMILTRKKGAYIRSDVTAKTDILVEGVQDDKYKDV
                     NGLVSKQRKAREYVGNGAKIQFLNEEDLINLIKE"
     gene            3425..3766
                     /locus_tag="O2701_002571"
     CDS             3425..3766
                     /locus_tag="O2701_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294437.1"
                     /translation="MIKKIFTKKHVFLVIEDENHNHSDAVFGKSILLSIYVGVNKKTN
                     SKSGKFIYLDRSKRIVRQSDITKIESANENDVDFYNLLKKEKEIVYSKNIVDKYNLAN
                     YIIYYEVSTKE"
     gene            3970..4152
                     /locus_tag="O2701_002572"
     CDS             3970..4152
                     /locus_tag="O2701_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294438.1"
                     /translation="MKITNCKIKRETVIYEVLTSGNQPFTYELPKDLSSHNARKYLEF
                     ISQKIDGDKLTKEDSL"
     gene            4230..>4652
                     /locus_tag="O2701_002573"
     CDS             4230..>4652
                     /locus_tag="O2701_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCZ4294439.1"
                     /translation="MTHNIEKRINKLKTSGNPKFKKLDSDIHYLLKRFEGEKNHKGFY
                     PKFKQGEIVFVDFGINVNKEFSNSHFAIVMNKNDSNTEDIVNVIPLSSKENKKYLKMN
                     FDLKWEYYLRLFLNLISAQNNSAILKEVFDKKYQKNNTE"
BASE COUNT         1659 a          690 c          709 g         1594 t
ORIGIN      
        1 ttcctccttt taagatgttt gtttttctcc taaaaactta ttaacaaagt attgttgtcc
       61 tttgcctgtt acttttggcg tcttactaat tgatgtgtga ccgtccgaat gtgtgattga
      121 tgtttcttta atttcgaata actcacgttc cattgaatac tgtgtaggca tgttataatc
      181 cacacccttg cgtttaataa ggaatccgtt ttgacgtaac cactcaaaca atctgcgttg
      241 cccgatgttt acaccgtttt gtttaatgat ctttgctaac tctccaacta aaattgatgt
      301 cttagtagta gctactgcat ctgcaaatac aatttttggt ttatcacgtt caatctttgt
      361 ttctaattga ttgattgtgt tgttagcaat ttttaaagca cgttgcataa tcatttctgg
      421 gctgttccat gctttttcaa cttggatgaa gtattgtctt gcacgtttac caggttcact
      481 acgttgaatc attgcgattt cttttgcagt gtctagtgtg agtgcgtggt caatatagtg
      541 tgtcatattg ccttgagctg ttgctctttt ttgagcgata gctgtgtaat ctgtattttc
      601 ttcaaatccg tatttaagca ttcttggaaa ccaatcttta tatgctgtct taacttctaa
      661 tgcttgatga agttctcgac cgctgattgc gatttctcca ttttcttttt cttgaatatt
      721 gaacatttct ccgatgttcg aatttgtttg taatgcttgc atattgttta tgctcctttc
      781 tgctatactc ctattaagga ggtgaatgac ttatgactaa tgaagctaaa tttgtccttt
      841 tacaacttta ttcaatttac cttgatagaa ttgacgaagg tatgtctaaa cgttctgcat
      901 cttatttcgg tagtgatgaa tcctcattta acgctttctt tttagatttt aattttgaag
      961 actatatcga tgcagttctt gaattaaagc atagagattt tgtaattgct tctgctgaag
     1021 atggcggttt tcttgagatg gctctttctc gagaaggtat cgcctactca gaatcagaat
     1081 ccaaaaaaga ttacaaaaca cttatggatt taattagaga tttgaaaaaa ttaataatct
     1141 aaaatccaat catctgctat taaatcgtct gcgctaggct gccaccttcc ggcggcggtt
     1201 tgtctttttt tcttatagtg tctagatacg actaggcatt gataacgctg caaattagtt
     1261 ggtaatatcc catacacatc ttgattctct ctccttatac ttattccttt ctccatcgct
     1321 agcttcgttg cttcttgaat gttcatttgt tattcctcct attaagatgt ttgtttttct
     1381 ttaaatgcta aaataattga tttcttttta tcattcgtaa atacgaaatt ttcgtattca
     1441 ttacctaaaa aaatatcatc atatttaaca ttaaaagcac tcatatactt agaaagtaaa
     1501 ctatctttaa tgtttgtaga gtctttttcc atattttgaa ttgtacgtga tgagacctta
     1561 aataaatctc ctaactcttt ttgagtcaat ccgtaatcag tcctcaactc ttttaatgtt
     1621 ttcatgttgt caccgccttt cgtaaaccta atataatacg aaattttcgt attgtcaaca
     1681 ttaaatacgt ttttttcgta aaaaacttta ctatgatatg aaaatttcgt ataataagaa
     1741 aaaaggaggt aagtaatatg aacaaagaaa gaaatattat tatagccaaa aacattagaa
     1801 aatttctcaa cgattcaaat atgtctcaaa agaaacttgc tgaactcatt aacataaaac
     1861 catctacttt aagcgattat ttaaatttac gttccaaccc ctctcacggc gttatacaaa
     1921 ggatagctga tgttttcgag gttggtaaaa gcgacataga tactacatac aaagacgata
     1981 acgacatcac ttccatatac aacaaactca cacctccccg ccaagaaaac gtacttaact
     2041 atgcaaatga acaattggaa gaacagaatt ctaaaggaga taacgttgta gatattaatt
     2101 catataaaca ggagaaaact ccagttaacg tcaatggttg cgtctctgct ggtgtaggag
     2161 aacgtttaca cgatgaaacg ctatttactg aaatggttaa aggacctatc cccacacacg
     2221 atttagcgtt aaaagtaaat ggtgattcta tggaacctat gtttaaagat ggcgaaatca
     2281 tatttgtgga gaaaactcac aatataaaga atggacaaat tggtatattc atcattgaag
     2341 aagaagcgta cgttaagaaa gtctttgttg aagatgatag attgactcta gtttcactaa
     2401 ataaagatta cgacgatcta cacttttata gaaatgaaag tgtgaggtta attggaaaag
     2461 ttattttata aaaggagcac ttgcaaatga aaaaatatga tattgcagtc ttagactttg
     2521 aaactatgaa tgaacatatg aacagccctt gcgaagttgc tgtatcttta attaaggatt
     2581 tatcaatagt aaaagtttat tcatcttata ttaatcctcc taataataga tataacttga
     2641 aaaacgctaa aatacataaa atacctgaag atgtcatatt aaaagcacct aaatatccag
     2701 atatttacca agaaattctc tatcttttaa aagaatcaca tttaattatt gctcataatg
     2761 cactttttga tatttcagta ttaaaaaata ctaataatta ttatgactta cctgttccaa
     2821 acttcatgta tgtcgatagt ataaatatct ttagaagctt ccacgcaatc tctagtttta
     2881 aattagaaaa tttgtgtagc ttatatgata tcgataaaga aaaattacat tctgctaaat
     2941 ttgacgtgct agctttatcg aagatgttga tatcactcgc taaaaacaat cagcattata
     3001 gtgtattaaa attaatacat tatatgccta agcaatacat tagatttagc aaatattcta
     3061 actctccaac taaacttttc gattcaggat ttcaaaaaat tcatatgaaa atatctgaga
     3121 ttaataaaat agaagtggaa agtgtaatcc ctattttaaa agataaaaat gttgttttta
     3181 caggtaattt tgacactgaa aaacaagatt taatgatatt aactagaaag aaaggagctt
     3241 atatcagaag tgacgtaact gcaaaaacag atattttagt cgaaggtgtt caagatgata
     3301 aatataaaga tgtgaacgga ctagtttcaa aacaacgaaa agctcgagaa tatgttggaa
     3361 atggtgcaaa aattcaattt ttaaatgaag aagacttaat aaatttaata aaggaataat
     3421 aacgatgatc aaaaaaattt ttacaaaaaa gcatgtattc ttagttatag aagatgaaaa
     3481 ccataatcac agtgatgctg tttttggaaa aagtatatta ctttcaattt acgtcggtgt
     3541 gaataaaaag actaattcta aatcagggaa atttatatac cttgacagat ctaaaagaat
     3601 cgttagacaa tctgatatca ccaaaataga atcagctaac gaaaatgatg tagattttta
     3661 taatttactg aagaaagaaa aggaaattgt ttattccaaa aatatagtag ataaatacaa
     3721 tttagcgaac tatataattt actacgaagt tagtactaaa gaataaacca atccattatt
     3781 tcataatact aaccttaaat ttacagaggt tttaattatg aaacatgaaa aaagcaatct
     3841 taactttaag tcttatattt attacctact acctcacttt taaatatatg tggattaaag
     3901 aattgaagta ttaattatgc ttatttaaaa aagacgtcta tttcagcagt gtttgaaagg
     3961 aagtttataa tgaaaataac taattgcaaa ataaaaagag aaactgtaat atacgaagtt
     4021 ttaactagtg gtaatcaacc attcacttat gagttaccta aagatttatc gtcacataat
     4081 gcgcgtaaat acttggaatt tatttcacaa aaaatagatg gcgataagtt aaccaaagaa
     4141 gattcattat gattttacta atcaaaaaac gtctacaagt gtagacgttg aatggtggtg
     4201 agagtgtgag cgagaataaa ggagaaatga tgacgcataa tatagaaaaa cgcattaata
     4261 aattaaaaac ttctggaaat ccaaaattta aaaaattaga ttcagatatt cactatttac
     4321 tcaagagatt tgaaggtgaa aaaaaccata aaggttttta tccaaagttt aaacaaggag
     4381 aaatagtttt tgtagatttc ggtataaacg ttaataaaga attctctaat tcacactttg
     4441 caatagtgat gaataaaaat gattctaata cggaagatat agtaaatgtt attcccttat
     4501 cttctaaaga aaacaaaaag tatttaaaga tgaattttga tttgaaatgg gagtattatt
     4561 taagattgtt tttaaattta attagcgcgc aaaataattc agctatatta aaagaagttt
     4621 tcgataaaaa ataccaaaaa aacaacacag aa
//
LOCUS       DALAGD010000142         4650 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.147, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000142 DALAGD010000000
VERSION     DALAGD010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.147"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            45..752
                     /gene="splA"
                     /locus_tag="O2701_002574"
     CDS             45..752
                     /gene="splA"
                     /locus_tag="O2701_002574"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplA"
                     /protein_id="HCZ4294440.1"
                     /translation="MNKNVMVKGLTALTILTSLGFAENISNQPHSIAKAEKNVKEITD
                     ATKEPYNSVVAFVGGTGVVVGKNTIVTNKHIAKSNDIFKNRVSAHHSSKGKGGGNYDV
                     KDIVEYPGKEDLAIVHVHETSTEGLNFNKNVSYTKFADGAKVKDRISVIGYPKGAQTK
                     YKMFESTGTINHISGTFMEFDAYAQPGNSGSPVLNSKHELIGILYAGSGKDESEKNFG
                     VYFTPQLKEFIQNNIEK"
     gene            877..1599
                     /gene="splB"
                     /locus_tag="O2701_002575"
     CDS             877..1599
                     /gene="splB"
                     /locus_tag="O2701_002575"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplB"
                     /protein_id="HCZ4294441.1"
                     /translation="MNKNVVIKSLAALTILTSVTGIGTTLVEEVQQTAKAENNVTKVK
                     DTNIFPYTGVVAFKSATGFVVGKNTILTNKHVSKNYKVGDRITAHPNSDKGNGGIYSI
                     KKIINYPGKEDVSVIQVEERAIERGPKGFNFNDNVTPFKYAAGAKAGERIKVIGYPHP
                     YKNKYVLYESTGPVMSVEGSSIVYSAHTESGNSGSPVLNSNNELVGIHFASDVKNDDN
                     RNAYGVYFTPEIKKFIAENIDK"
     gene            1657..2376
                     /gene="splC"
                     /locus_tag="O2701_002576"
     CDS             1657..2376
                     /gene="splC"
                     /locus_tag="O2701_002576"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplC"
                     /protein_id="HCZ4294442.1"
                     /translation="MNKNIVIKSMAALAILTSVTGINAAVVEETQQIANAEKNVTQVK
                     DTNIFPYNGVVSFKDATGFVIGKNTIITNKHVSKDYKVGDRITAHPNGDKGNGGIYKI
                     KSISDYPGDEDISVMNIEEQAVERGPKGFNFNENVQAFNFAKDAKVDDKIKVIGYPLP
                     AQNSFKQFESTGTIKRIKDNILNFDAYIEPGNSGSPVLNSNNEVIGVVYGGIGKIGSE
                     YNGAVYFTPQIKDFIQKHIEQ"
     gene            2497..3216
                     /gene="splD"
                     /locus_tag="O2701_002577"
     CDS             2497..3216
                     /gene="splD"
                     /locus_tag="O2701_002577"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplD"
                     /protein_id="HCZ4294443.1"
                     /translation="MNKNIIIKSIAALTILTSITGVGTTVVDGIQQTAKAENSVKLIT
                     NTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVTYHMKVGDEIKAHPNGFYNNGGGLYK
                     VTKIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFTSKFNIASEAKENEPISVIGYPNPN
                     GNKLQMYESTGKVLSVNGNIVTSDAVVQPGSSGSPILNSKREAIGVMYASDKPTGEST
                     RSFAVYFSPEIKKFIADNLDK"
     gene            complement(3165..3272)
                     /locus_tag="O2701_002578"
     CDS             complement(3165..3272)
                     /locus_tag="O2701_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294444.1"
                     /translation="MLILLLISKNHLSMYGWMIYLSKLSAINFLISGEK"
     gene            3374..4090
                     /gene="splE"
                     /locus_tag="O2701_002579"
     CDS             3374..4090
                     /gene="splE"
                     /locus_tag="O2701_002579"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplE"
                     /protein_id="HCZ4294445.1"
                     /translation="MNKNIIIKSIAALTILTSVTGVGTTVVEGIQQTAKAEHNVKLIK
                     NTNVAPYNGVVSIGSGTGFIVGKNTIVTNKHVVAGMEIGAHIIAHPNGEYNNGGFYKV
                     KKIVRYSGQEDIAILHVEDKAVHPKNRNFKDYTGILKIASEAKENERISIVGYPEPYI
                     NKFQMYESTGKVLSVKGNMIITDAFVEPGNSGSAVFNSKYEVVGVHFGGNGPGNKSTK
                     GYGVYFSPEIKKFIADNTDK"
     gene            4241..>4650
                     /gene="splF"
                     /locus_tag="O2701_002580"
     CDS             4241..>4650
                     /gene="splF"
                     /locus_tag="O2701_002580"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplF"
                     /protein_id="HCZ4294446.1"
                     /translation="MNKNIIIKSIAALTILTSITGVGTTMVEGIQQTAKAENTVKQIT
                     NTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVTYHMKVGDEIKAHPNGFYNNGGGLYK
                     VTKIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFT"
BASE COUNT         1833 a          665 c          835 g         1317 t
ORIGIN      
        1 ctagatacat taactataaa aataaatttg gaaggaggta aaatatgaat aaaaatgtaa
       61 tggttaaagg tttaactgct ttaactattt taacatctct tggttttgct gaaaatattt
      121 ctaatcagcc tcattcaatt gccaaagcag aaaagaatgt caaagaaatt accgatgcaa
      181 ctaaggaacc atacaattca gtggtagcat ttgtgggtgg tactggtgta gttgttggta
      241 aaaatacaat cgtaactaac aaacatatcg ctaaaagtaa tgatattttt aaaaatagag
      301 tatcagcaca tcattcgagt aaaggtaaag gcggaggaaa ctacgacgtt aaagacattg
      361 tagaatatcc cggaaaagaa gaccttgcga tagttcatgt tcatgaaaca agtacagaag
      421 gtttgaattt taataagaac gttagttata caaaatttgc agacggagca aaagtgaaag
      481 atagaatttc tgttattggt tatccaaagg gtgcacaaac aaaatataaa atgtttgaat
      541 cgacaggaac gattaaccat atcagtggaa cgtttatgga atttgatgcg tatgcacaac
      601 caggtaattc aggatctcct gtattgaatt ctaaacatga actgattggt attttatatg
      661 caggtagtgg aaaagatgaa tctgaaaaga atttcggtgt ttatttcaca ccacaattaa
      721 aagaatttat tcaaaataat attgaaaaat aagtatcatc aattcattcg tgaagttgat
      781 tttttaaaga gtgattaaga aaacggttac ataattaact aaatatactg aattatgtat
      841 ctagatatca aaataattaa aagagaggaa cttaaaatga acaaaaacgt agtcatcaag
      901 agtttagcag cattaacaat tttaacatct gtaacaggta ttggaacaac attggttgag
      961 gaagtacaac aaactgccaa agcagaaaat aatgtcacaa aagttaaaga tactaatatt
     1021 tttccatata ctggtgtagt tgcttttaaa agtgcaactg gatttgtagt tggaaagaat
     1081 actattttaa caaataaaca tgtgtcgaaa aattacaaag tgggcgatcg tattactgca
     1141 catccaaata gtgataaagg taatggtggt atttattcga ttaaaaagat tattaattat
     1201 ccaggtaaag aagatgtatc agtcattcaa gttgaagagc gtgcaataga acgtggacca
     1261 aaaggcttta attttaatga taatgtaacg ccattcaaat atgcggcagg ggctaaagct
     1321 ggtgagcgaa ttaaagtgat tggttatcca cacccataca aaaataaata tgttttatat
     1381 gagtcaactg gccctgtgat gtcagtagaa ggtagcagta ttgtatattc agcgcatact
     1441 gaaagcggaa actctggatc acctgtatta aacagcaaca acgaattagt tggtattcat
     1501 tttgcttctg atgtaaaaaa tgatgataac agaaatgcat atggcgtcta ctttacacca
     1561 gaaattaaaa aattcattgc agaaaacata gataaataaa caaattgact ttaaacgagc
     1621 gttgcaacat atctcgaatt gtaaaggagc ttgaaaatga ataaaaatat agtcattaaa
     1681 agcatggcag cattagccat tctaacctca gtaactggaa taaatgctgc agtcgttgaa
     1741 gagacacaac aaatagcaaa tgcagagaag aatgttacgc aagttaaaga tacaaatatt
     1801 tttccatata atggcgtcgt ttcatttaaa gatgcgacag gttttgtaat tggaaaaaat
     1861 acaattatca ccaataaaca tgtatcaaaa gattataaag ttggcgatag aattactgcc
     1921 catccaaacg gtgacaaagg aaatggtggt atatataaaa ttaaaagcat ttctgattat
     1981 ccgggtgatg aagacatctc tgtcatgaat attgaagaac aagcagtcga acgtggacca
     2041 aaaggcttta attttaatga aaatgtccaa gcattcaatt ttgcgaaaga tgctaaagtt
     2101 gatgacaaaa ttaaagttat tggttaccca ttacctgctc aaaatagttt taaacagttt
     2161 gaatctacag gaactataaa aagaatcaaa gacaatattt taaattttga tgcatacatt
     2221 gaacccggga attcaggatc accagttcta aattctaaca atgaggtcat aggtgtggtg
     2281 tatggcggta ttggaaaaat tggttctgaa tataatggtg ccgtatactt tacgcctcaa
     2341 atcaaagatt ttattcaaaa gcacattgaa caataaacaa atttaaatat acaccatgag
     2401 catgtgttca ataattttaa tgaaaaacat cggtcgaata taacataaaa aaacgtctat
     2461 atcaaaagca tcatgaataa acagaggagc acaaaaatga ataaaaatat aatcatcaaa
     2521 agtattgcgg cattgacgat tttaacatca ataactggtg tcggcacaac agtggttgat
     2581 ggtattcaac aaacagccaa agcagaaaat agtgtgaaat taattaccaa cacgaatgtt
     2641 gcaccataca gtggtgttac atggatgggc gctggaacag gatttgtagt tgggaatcat
     2701 acaatcatta ccaataaaca tgttacttat cacatgaaag tcggtgatga aatcaaagca
     2761 catcctaatg gtttttataa taacggtggt ggactttata aagttactaa gattgtagat
     2821 tatcctggta aagaagatat tgcggtcgta caagttgaag aaaaatcaac gcaaccaaaa
     2881 ggtagaaaat tcaaagattt cactagcaaa tttaatatag catcagaagc taaagaaaat
     2941 gaacctatat cagtcattgg ttatccaaat cctaatggaa ataaactaca aatgtatgaa
     3001 tcaactggta aagtactatc agtgaatgga aatatagtga catctgatgc ggttgtccaa
     3061 cctggcagct ctggttcacc tatattaaat agtaagcgag aagcaattgg tgttatgtat
     3121 gctagtgata aaccaacagg tgaaagtaca aggtcatttg ctgtttattt ctctcctgaa
     3181 attaagaaat ttattgcaga taatttagat aaataaatca tccatccata cattgataaa
     3241 tgatttttag aaattaacaa caaaatcaac aattttaaac atctctgtga ttctatttat
     3301 tcgaaatgat ttaaaaaata aaacttcaaa aacctaacct tatatttata cgaatactta
     3361 gaggagcaca aaaatgaata aaaatataat catcaaaagt attgcagcat tgacgatttt
     3421 aacatcagtg actggcgtcg gcacaacagt ggttgagggt attcaacaaa cggctaaagc
     3481 tgaacataat gtgaaactaa tcaaaaatac taatgtagca ccatacaatg gtgtcgtttc
     3541 gataggatct ggaacaggtt tcattgtcgg taaaaataca attgttacca acaagcatgt
     3601 cgttgcaggt atggaaattg gtgcacatat tatagcgcat cccaatggtg aatataataa
     3661 tggcggattt tataaagtta aaaaaattgt ccgttattca ggtcaagaag atattgccat
     3721 tctacatgtg gaagataaag ctgttcatcc aaaaaacagg aattttaaag attacacagg
     3781 cattttaaaa atagcatcag aagctaaaga aaatgaacgc atttcaattg ttggctatcc
     3841 agaaccatat ataaataaat ttcaaatgta tgagtcaaca ggaaaagtgc tgtcagttaa
     3901 aggcaacatg attattactg atgctttcgt agaaccaggc aactcaggtt cagctgtatt
     3961 taacagtaaa tacgaagttg taggtgttca ctttggtgga aacggccctg gaaataaaag
     4021 tacaaaagga tatggtgttt atttctctcc tgaaattaag aaattcattg cagataacac
     4081 agataaataa atccttacat agataaatga ttttaaaaat taacaacaaa ctcaacaatt
     4141 caaatcatct ctgtgattcc atttattcga aatgattaaa aaaaataaaa cttcaaaaag
     4201 ctaacattat aattatacaa atacttagag gagcagaaaa atgaataaaa atataatcat
     4261 caaaagtatt gcagcattga cgattttaac atcaataact ggtgtcggca caacaatggt
     4321 tgaaggtatt caacaaacag ccaaagccga aaatactgtt aaacaaatta caaatacaaa
     4381 tgttgcacca tacagtggtg ttacatggat gggcgctgga acaggatttg tagttggaaa
     4441 tcatacaatc attaccaata aacatgttac ctatcacatg aaagtcggtg atgaaatcaa
     4501 agcacatcct aatggttttt ataataacgg tggtggactt tataaagtta ctaagattgt
     4561 agattatcct ggtaaagaag atattgcggt tgtacaagtt gaagaaaaat caacacaacc
     4621 aaaaggtaga aaattcaaag atttcactag
//
LOCUS       DALAGD010000143         4645 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.148, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000143 DALAGD010000000
VERSION     DALAGD010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.148"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            180..1355
                     /gene="rpsA"
                     /locus_tag="O2701_002581"
     CDS             180..1355
                     /gene="rpsA"
                     /locus_tag="O2701_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCZ4294447.1"
                     /translation="MTEEFNESMINDIKEGDKVTGEVQQVEDKQVVVHINGGKFNGII
                     PISQLSTHHIDSPSEVVKEGDEVEAYVTKVEFDEENETGAYILSRRQLETEKSYSYLQ
                     EKLDNNEIIEAKVTEVVKGGLVVDVGQRGFVPASLISTDFIEDFSVFDGQTIRIKVEE
                     LDPENNRVILSRKAVEQEENDAKKDQLLQSLNEGDVIDGKVARLTQFGAFIDIGGVDG
                     LVHVSELSHEHVQTPEEVVSIGQDVKVKIKSIDRDTERISLSIKDTLPTPFENIKGQF
                     HENDVIEGVVVRLANFGAFVEIAPGVQGLVHISEIAHKHIGTPGEVLEPGQQVNVKIL
                     GIDEENERVSLSIKATLPNEDVVESDPSTTKAYLENEEEDNPTIGDMIGDKLKNLKL"
     gene            1577..2887
                     /gene="der"
                     /locus_tag="O2701_002582"
     CDS             1577..2887
                     /gene="der"
                     /locus_tag="O2701_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019167598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCZ4294448.1"
                     /translation="MTKPIVAIVGRPNVGKSTIFNRIVGERVSIVEDTPGVTRDRIYS
                     SGEWLTHDFNIIDTGGIEIGDAPFQTQIRAQAEIAIDEAYVIIFMVNVREGLTQSDEM
                     VAQILYKSKKPVVLAVNKVDNMEMRTDVYDFYSLGFGEPYPISGSHGLGLGDLLDAVV
                     SHFGEEEEDPYDEDTIRLSIIGRPNVGKSSLVNAILGEDRVIVSNVAGTTRDAIDTEY
                     SYDGQDYVLIDTAGMRKKGKVYESTEKYSVLRALKAIERSNVVLVVIDAEQGIIEQDK
                     RVAGYAHEQGKAVVIVVNKWDTVEKDSKTMKKFEDEVRKEFQFLDYAQIAFVSAKERT
                     RLRTLFPYINEASENHKKRVQSSTLNEVVTGAISMNPTPTDKGRRLNVFYATQVAIEP
                     PTFVVFVNDVELMHFSYKRYLENQIRAAFGFEGTPIHIIARKRN"
     gene            2904..3902
                     /locus_tag="O2701_002583"
     CDS             2904..3902
                     /locus_tag="O2701_002583"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCZ4294449.1"
                     /translation="MTKITVFGMGSFGTALANVLAENGHDVLMWGKNQDAVDELNTCH
                     TNKKYLKYAKLDVNIIATSDMTKAIQFADIYLMALPTKAMREVASQINDKLTSKKTFI
                     HVAKGIENGTFKRVSEMIEDSISPEYNAGIGVLSGPSHAEEVVVKQPTTVAASSKDKS
                     VSKLTQDLFMNDYLRVYTNDDLIGVELGGALKNIIAVASGIVAGIGYGDNAKAALMTR
                     GLAEISRLGEKLGADPMTFLGLGGIGDLIVTCTSTHSRNFTLGYKLGQGESMDQALSE
                     MNMVVEGIYTTKSVYHLAKEKNVDMPITNALYRVLFENISVKECVKDLMERDKKSE"
     gene            4073..4345
                     /locus_tag="O2701_002584"
     CDS             4073..4345
                     /locus_tag="O2701_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HCZ4294450.1"
                     /translation="MNKTDLINAVAEQADLTKKEAGSAVDAVFESIQNSLAKGEKVQL
                     IGFGNFEVRERAARKGRNPQTGKEIDIPASKVPAFKAGKALKDAVK"
BASE COUNT         1673 a          613 c          923 g         1436 t
ORIGIN      
        1 attactttga tacaaaaatg gttgtaataa atatttatcg atatgacgac ttgaatatga
       61 taaagtgaca tatttatgta tatgactatt tcgcaaaatg taatcgaggt agaatttctt
      121 gacaattctg tcagtttata agatgttata aatatgtagt gtataaggag gcaaacaaga
      181 tgactgaaga attcaatgaa tcaatgatta acgatattaa agaaggtgac aaagtcactg
      241 gcgaggtaca acaagttgaa gacaagcaag ttgttgttca tatcaacggt ggtaaattta
      301 atgggattat tcctattagt caattatcta cgcatcatat tgatagccca agtgaagttg
      361 taaaagaggg cgacgaagtt gaagcatatg tcactaaagt tgagtttgat gaagaaaatg
      421 aaactggagc ttacatctta tctagaagac aacttgaaac tgagaagtct tatagttatt
      481 tacaagaaaa attagataat aatgaaatca tcgaagcgaa agtaacagaa gtagttaaag
      541 gtggtttggt tgttgatgta ggacaaagag gttttgttcc ggcttcacta atttcaacag
      601 acttcattga ggatttctct gtgtttgatg gacaaacaat tcgtattaaa gttgaagaat
      661 tggatcctga aaataataga gtcattttaa gccgtaaagc agttgaacaa gaagaaaacg
      721 atgctaaaaa agatcaatta ttacaatctt taaatgaagg cgatgttatt gatggtaaag
      781 tagcgcgttt aactcaattt ggtgcattta tagacattgg cggtgttgat ggtttagtgc
      841 atgtatctga actttctcac gaacatgttc aaacaccaga agaagtagtt tcaattggtc
      901 aagatgttaa agttaaaatt aaatctattg atagagatac agaacgtatt tcattatcaa
      961 tcaaagatac gttaccaaca cctttcgaaa atattaaagg tcaattccac gaaaatgatg
     1021 tcattgaagg tgtcgtagta agattggcaa actttggtgc atttgttgaa attgcaccag
     1081 gtgtacaagg acttgtacat atttctgaaa ttgcacacaa acacattggt acgccaggtg
     1141 aagtgttaga acctggtcaa caagtaaatg ttaaaatatt aggtattgat gaagagaatg
     1201 aaagagtatc actatctatt aaagcaacat taccaaacga agatgttgtt gaaagtgatc
     1261 cttctacgac taaggcgtac ttagaaaacg aagaagaaga taatccaaca attggcgata
     1321 tgattggtga taaacttaaa aatcttaaac tataatttaa tatttaatag tcaactccac
     1381 atgtttatga ttgcatgtgg agtattttta tgtaacaaaa tatactcgga atgataacgt
     1441 ggacaaattt aactaagtgt ttaaaaagat aaagttttaa gtgctgattt ttatcattac
     1501 agtaataaac tcattttgaa tacacagtct catgtgatat tattaaaaag atataagaaa
     1561 gagaggaagt tagcttatga ctaaacctat agtagctatt gtaggtaggc ctaatgtagg
     1621 taaatctaca atttttaata gaatagttgg agaacgtgtt tcgattgtgg aagacacgcc
     1681 aggtgtaaca cgagatcgta tttattcttc aggtgaatgg ttaacacatg atttcaatat
     1741 tattgataca ggtggtattg aaattggtga tgcaccattc caaacacaaa ttagagcgca
     1801 ggcagaaatc gccatagatg aagcgtatgt tattattttt atggttaacg tgcgtgaagg
     1861 attgacacaa agcgatgaaa tggtcgctca aattttatac aaatctaaaa aaccggtcgt
     1921 attagcggtt aacaaagtag ataatatgga aatgcgtaca gacgtgtatg atttctattc
     1981 attaggattt ggtgaaccgt atccgatatc agggtcacat ggtttaggtc ttggtgactt
     2041 gttagatgca gttgtttctc attttggtga agaggaagaa gatccttatg atgaagatac
     2101 aattcgacta tccattattg gacgaccaaa cgtaggtaaa tcaagtttag taaatgctat
     2161 tttaggtgaa gatcgcgtta tcgtttctaa tgttgcaggg acaacgagag acgctattga
     2221 tacagagtat agttatgatg gacaagatta tgttttaatc gatactgctg gtatgcgtaa
     2281 aaaaggaaaa gtatatgaat caactgagaa atattcagta ttaagagctt taaaagcgat
     2341 tgaacgttca aatgttgttt tagtggtcat agatgcagaa caaggcatca ttgaacaaga
     2401 taaacgtgtt gcaggatatg cacatgaaca aggtaaagca gtcgtgattg tcgtaaataa
     2461 atgggatact gtggaaaaag atagtaaaac gatgaagaaa tttgaagatg aagtacgtaa
     2521 agaattccaa tttttagatt atgcacaaat tgcttttgtg tctgctaaag aacgcacaag
     2581 attacgtaca ttattccctt acatcaatga agcaagtgaa aaccataaaa aacgtgttca
     2641 aagttcaact ttaaatgaag ttgttactgg tgcaatttcc atgaacccta caccaacaga
     2701 caaaggtaga cgtttgaatg tcttttatgc aacacaagtt gctatagaac caccgacatt
     2761 tgttgtattt gttaatgatg tagaattaat gcatttttct tataaacgct atttagagaa
     2821 tcaaatccgt gccgcttttg gttttgaagg tacaccaatt catattatag ctcgaaagag
     2881 aaattaacga ttgggggata acaatgacta aaattaccgt ttttggtatg ggaagttttg
     2941 ggacagccct tgccaatgtt cttgcagaaa atggacatga tgttttgatg tggggtaaaa
     3001 atcaagatgc tgttgatgaa ttaaatacat gtcatacaaa taaaaagtat ttaaaatacg
     3061 cgaaattaga tgttaacatc atcgctactt cagatatgac caaagcaatt caatttgcag
     3121 atatttactt aatggcttta cctactaaag caatgcgaga agttgcttct caaattaatg
     3181 ataagctgac ctctaaaaag acttttatac atgttgctaa aggtattgaa aatgggacgt
     3241 ttaaacgtgt gtcagaaatg attgaagatt ctatttcacc tgaatataat gcaggtattg
     3301 gcgtgttgtc agggccaagt catgcggaag aagttgtagt caagcaacca actacagttg
     3361 ctgcttcatc aaaagataaa agtgtaagta aattaacgca agatttattt atgaatgatt
     3421 atttgcgtgt gtacacgaat gatgacttga ttggtgttga acttggtggt gcattgaaaa
     3481 atatcatcgc agtagcaagt ggtatcgtag ctggaattgg ctacggtgat aatgcaaaag
     3541 ctgcattaat gactcgtggc ttagcggaaa ttagtagatt aggtgaaaag ttaggtgccg
     3601 atcctatgac atttctaggt ttaggtggta tcggtgactt aatcgttact tgcacatcaa
     3661 cacattctcg gaatttcaca ttaggatata aacttggaca aggtgaatca atggatcaag
     3721 cattatctga aatgaatatg gttgttgaag gtatttatac aactaaatca gtttatcatt
     3781 tagctaaaga aaaaaatgtg gatatgccaa ttacaaatgc attatataga gtattatttg
     3841 aaaatatctc agtaaaagaa tgcgtaaaag atttaatgga gcgcgataaa aaatctgaat
     3901 aaaatgtgta ttattacaca tatacggcta ataattacgt aaaatgaata gaaatggctt
     3961 aaattcaacg tttttgccac ataaatgatt gcgatttatg taatgcttgt gttaaagttt
     4021 taccataatg atataaacat atcattatca aacctcagga ggtgaatgtc taatgaacaa
     4081 aacagattta atcaatgcag ttgcagagca agctgattta actaaaaaag aagctggttc
     4141 agcagtagat gctgtattcg aatcaatcca aaactcactt gctaaaggtg aaaaagtaca
     4201 attaattggt ttcggtaact ttgaggtacg tgaacgtgct gcacgtaaag gtcgtaaccc
     4261 tcaaactggt aaagaaattg atatcccagc aagtaaagtt ccagcattca aagctggtaa
     4321 agcattaaaa gatgctgtaa aataatttta cttaaaaagc cctgaataag ggctttttat
     4381 tttgctttta atacttacaa cctgtacata aattgtaatg ttcttctaag tttttaatct
     4441 ttggaacatt cattgtttaa gcgttcaaaa tattacatga cataatagtt ttgataaaaa
     4501 tagcaactac aaaatataca tatgaatatt gagattaatt gttagcgttg aatttactta
     4561 aaaggtaacc atgtctacta tagtatttta cgttatttaa aaagatgaat aatgtaaatg
     4621 aagtaaaggt tattatgaga attac
//
LOCUS       DALAGD010000144         4525 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.149, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000144 DALAGD010000000
VERSION     DALAGD010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.149"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            120..770
                     /locus_tag="O2701_002585"
     CDS             120..770
                     /locus_tag="O2701_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HCZ4294451.1"
                     /translation="MKITYKYRGDLPLNTENNKNQNQSVKNSERRGMLKGCGGCLISF
                     ILLIILLSACSMMFSNNDNSTNNQSSKTQLTQKDENKNEDKPEEKSETATDEDLQSTE
                     EVPANENTENNQHEIDEITTKDQSDDDINTPNVAEDKSQDDLKDDLKEKQQSSNHHQS
                     TQPKTSPSTETNTQQSFANCKQLRQVYPNGVTADHPAYRPHLDRDKDKRACEPDKY"
     gene            851..1846
                     /locus_tag="O2701_002586"
     CDS             851..1846
                     /locus_tag="O2701_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HCZ4294452.1"
                     /translation="MSNQFKSEEERRQWEQFQAFQNQQNQQNQQYGQKKSKKGWFWGC
                     GGCLVLFILIIIGISACTAGITGNLGGNSSKETNKTHKIGETVKNGDLEVTVNSVETM
                     KSVGPSLAPTNAKGIFVVADVTIKNKGKEALTIDSSMFKLKSGDKTFEADNTGSMSAN
                     QSDNGSIENSFFLQRINPDSTAQGKIVFDVSENIANAKDKKLEVISSLFSVKKITFDL
                     SDAKKTSKAKKDKQDTEVAVASSNSDNVSYEASATTPATTSSADTDSEDSEKSSKDED
                     KQNASKSDKSSVEKSESNEETAPVEPMPHSKPTTSEAPPSQNIHNEDSMYDASTE"
     gene            1922..2548
                     /locus_tag="O2701_002587"
                     /pseudo
     CDS             1922..2548
                     /locus_tag="O2701_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500421.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2589..2933
                     /locus_tag="O2701_002588"
     CDS             2589..2933
                     /locus_tag="O2701_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3969 family protein"
                     /protein_id="HCZ4294453.1"
                     /translation="MITFENIQQLEKYTLMTMHGLFNQLKLGIISIENAEHTLFTPYM
                     LETLSSLGVKDSIVDLIHKGTELEDFAAFNLSIEDTVTVCLQRTEELLKQYKNVEFND
                     KILINWRIIQEK"
     gene            3031..3582
                     /locus_tag="O2701_002589"
     CDS             3031..3582
                     /locus_tag="O2701_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294454.1"
                     /translation="MEKNEYIAKYNEYSQLLDATYSQAVAYLLSKYGAVTDDYYKEKS
                     YTRFLNGEIKSISKGKYTRASEGLYCHHISEDKFQNLSDLRFISKFKYSYDVQKKENL
                     VYCDLIEHLILHAIITKESHGQFGVAGLCQMIKPTVIDWYIGEYNPKPAWMQATKARA
                     YLPGILVEKLLIKIDDMLKGIEI"
     gene            complement(3800..4441)
                     /locus_tag="O2701_002590"
     CDS             complement(3800..4441)
                     /locus_tag="O2701_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCZ4294455.1"
                     /translation="MFTPIVINLLNDVGSIFLVRVGNQLKVIEKVHYEDILESKIVRL
                     FQAFIKDKMDQLFITSEHFAIDIDKLLFQLNLNVRYIKMEGNTSGYLSGRTIYINNNH
                     SINNTRFSIARELGRYLFKNTDLMRDSNDNSLKNLHEMIYESDVNQFSVDLLMPKKQI
                     EALVYNFYEVNNINLSSGLSEKERNKLLNLISTKLEVSKVAAGLRLYNLGIHI"
BASE COUNT         1798 a          710 c          706 g         1311 t
ORIGIN      
        1 tgaaaaaaat taaagtattt ccactactaa tttggtaaaa tatgattatg tgatatagtg
       61 aaagcgtttt acacttaata actccctctt aaatgcatcc aggttttatg tagtaaatca
      121 tgaaaataac atataaatat agaggagatt tacctttgaa tacagagaac aacaagaatc
      181 aaaaccaatc tgttaaaaat tctgaaagac gcggcatgtt aaaaggatgc ggcggttgcc
      241 ttatttcttt tattttatta ataatcttat tatcagcctg ttcaatgatg tttagtaata
      301 atgacaattc cactaataat caatcatcaa aaacgcaatt aactcaaaaa gatgaaaata
      361 aaaatgaaga taagcctgag gaaaaatcag aaacagcaac agatgaggat ttacaatcaa
      421 ccgaagaagt acctgcaaat gaaaatactg aaaataatca acatgaaatt gatgaaataa
      481 caacaaaaga tcaatcagac gatgatatta acacaccaaa cgttgcagaa gataaatcac
      541 aagacgactt gaaagatgat ttaaaagaaa agcaacaatc aagtaaccat catcaatcca
      601 cgcaacctaa gacctcacca tcaactgaaa caaacacgca acaatcattt gctaattgta
      661 agcaacttag acaagtatat ccgaatggtg tcactgccga tcatccagca tatcgaccac
      721 atttagatag agataaagat aaacgtgcat gtgaacctga taaatattaa acaacaagcg
      781 aattgaattc aaattgtatt tagctttatg cactaatcac atagtaaata atgagggaga
      841 ttttttaggc atgagcaatc aattcaaaag cgaagaagag cgaagacaat gggaacaatt
      901 ccaagctttc caaaatcaac aaaaccaaca gaaccagcaa tacggacaaa agaaatctaa
      961 aaaaggatgg ttctggggct gtggtggttg tctagtatta tttattttaa ttatcatcgg
     1021 tatttcagct tgtacagctg gtattacagg taaccttggc ggaaatagtt ctaaagaaac
     1081 gaacaaaacc cataaaatcg gtgaaactgt taaaaatggc gaccttgaag tcactgtaaa
     1141 ttcagtggaa actatgaaat ctgtaggacc atctcttgca ccaacaaacg ctaaaggtat
     1201 atttgtcgtt gctgatgtga cgattaaaaa caaaggtaaa gaagcgttaa caattgatag
     1261 ttcaatgttt aagctaaaat ccggtgataa aacatttgaa gcagataata caggttcaat
     1321 gtctgctaat caaagtgaca atggtagtat agaaaattca tttttcttac agcgtataaa
     1381 tccagatagc actgctcaag gtaaaattgt tttcgatgtg tcagaaaaca tagccaacgc
     1441 aaaagataaa aaattagaag ttatttctag tttatttagc gtcaagaaga ttacatttga
     1501 tttatccgat gctaaaaaaa catcaaaagc taaaaaagac aagcaagata cagaagtagc
     1561 tgttgcgagt tcaaatagcg ataatgtaag ttatgaagct tcggctacta cacctgctac
     1621 aacttctagt gcggatactg attctgaaga tagcgaaaag tctagtaaag atgaggataa
     1681 gcagaatgcg tctaaaagtg ataaatctag tgtagaaaaa agtgaatcta atgaggaaac
     1741 tgctcctgta gagcccatgc cccatagcaa acctaccact agtgaagcac cacctagcca
     1801 aaatattcac aatgaagata gcatgtacga cgcttcaaca gaataaaatt tcagggtagc
     1861 tcggctaccc ttcttttacg gaaaaattaa ttatacataa tcaaatcaag gagataaaaa
     1921 aatgaaattc aaagctatcg ttgcaatcac attatcattg tcactattaa ctgcctgtgg
     1981 tgctaatcaa cataaagaaa atagtagtaa atcaaatgac actaataaaa agacgcaaca
     2041 aactgacaac actacacagt caaatacaga aaagtaaatg acaccacaag aagccgaaga
     2101 tatagttcga aacgattaca aagcaagagg cgttaatgaa tatcaaacat taaattataa
     2161 aacaaatctt gaacgaagca atgaacatga atattatgtt gaacatctag tccgcgatgc
     2221 agttggcaca ccattaaaac gttgtgctat tgttaatcga cacaatggca caattattaa
     2281 tatttttgat gatatgtcag aaaaagacaa agaagaattt gaagcattta aaaagagaag
     2341 ccctaaatac aatccaggta tgaacaatca tgatgaaaca gatggtgagt cagaagacat
     2401 tcaacatcat gacattgata ataacaaagc aattcaaaat gacataccag atcaaaaagt
     2461 cgacgataaa aatgataaaa atgctgttaa taaagaagaa aaacatgata atggggcaaa
     2521 taattctgaa gaaactaaag ttaaataatg gcatactttg attaatcgta atttttagga
     2581 gaaacgctat gataacattt gagaatatac aacaacttga gaaatacacc ttaatgacta
     2641 tgcatggtct ttttaatcaa cttaaactcg gcattatttc aatcgaaaac gcagagcata
     2701 cgctttttac accttatatg ttggaaacgc tctcttccct aggcgtgaaa gacagcattg
     2761 tcgatttaat tcataaaggg actgaattag aagactttgc ggcatttaat ttatcaattg
     2821 aagacacagt tacagtctgt ttacaaagaa ctgaagaact attaaaacaa tacaaaaatg
     2881 tggaattcaa tgacaaaata ttaatcaatt ggcgtattat acaagagaaa tagacatata
     2941 aaagtcgaat gtaactacgt gagtattgat tttattcttt gtaaattaca agcatttcat
     3001 attataaagt ttgaaaagag gtatattgaa atggagaaaa atgaatatat agctaaatat
     3061 aatgaatata gtcaattatt agacgctaca tactcgcaag ctgtagcata ccttttaagt
     3121 aaatatggcg ctgtaaccga tgattattat aaagaaaaat catacacgcg atttttaaat
     3181 ggagaaatca aaagtatttc aaaaggaaaa tacactagag ctagcgaagg attatattgc
     3241 catcatataa gcgaggacaa attccaaaat ctatctgatc taagattcat ttccaaattt
     3301 aagtactcat acgacgttca aaagaaagaa aacttagtgt actgtgatct aatcgagcat
     3361 ttaattttac atgcaattat tacaaaagaa tcccatggcc aatttggtgt agctggatta
     3421 tgtcaaatga tcaaaccaac agtcattgat tggtacattg gcgaatataa tccaaaacca
     3481 gcatggatgc aagccaccaa agcacgtgcc tatttgcctg gaatattagt agagaaatta
     3541 ctcattaaaa ttgacgatat gttaaaagga atagaaatat aagatttcct tgagtctaga
     3601 taaatgatta atgtagattt attttttgct gttgagattt tgttatagat gtttaaacct
     3661 gtaattaaat atattttata aaatagacca cgcataccta tctataaacg gacaatgttt
     3721 ataaatgagt ttgcatggtc ttgaattgta ttaaatttct tttggtttta ataaatcgac
     3781 tagattttca caatatttat caaatatgta ttcctaaatt atacagcctt aatccagcag
     3841 ctactttcga aacttccaac ttagttgata taaggttcaa tagtttgttt cgttcttttt
     3901 cagataaacc agaacttaaa ttgatattat tgacttcata aaaattatag actaatgcct
     3961 ctatttgctt tttaggcata agtaagtcga ctgaaaactg atttacgtcg ctttcataaa
     4021 tcatttcatg taaattcttt agactattat cgttactatc tctcattaag tctgtatttt
     4081 taaataaata acggcccaat tcacgagcta ttgaaaatct tgtattatta atcgagtgat
     4141 tattattaat atagattgtt cttccactta aataacccga agtattaccc tccattttaa
     4201 tatatctaac atttaaatta agttgaaata atagcttgtc tatgtcaata gcaaagtgtt
     4261 cagaagtaat aaaaagttga tccattttgt cctttataaa tgcctgaaat aatcgaacta
     4321 tttttgattc taaaatatct tcataatgaa ctttctcaat aactttcaat tgattcccca
     4381 ccctaactaa aaaaatcgaa cctacatcat ttaataaatt tataactata ggtgtaaaca
     4441 ttattaaaat aaaacccctc tactactata tgtaacgaag ggacatgatt tcaaaataaa
     4501 ataccttttt tataaattta ttata
//
LOCUS       DALAGD010000145         4471 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.150, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000145 DALAGD010000000
VERSION     DALAGD010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.150"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(7..162)
                     /locus_tag="O2701_002591"
     CDS             complement(7..162)
                     /locus_tag="O2701_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294456.1"
                     /translation="MIIHCSIVSNFIFNLYIVTNFNLNFLSLKQIIYSKLIVRKFKIF
                     VDILKQI"
     gene            200..661
                     /gene="tsaE"
                     /locus_tag="O2701_002592"
     CDS             200..661
                     /gene="tsaE"
                     /locus_tag="O2701_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCZ4294457.1"
                     /translation="MIKINNLDEMNQFAIFLVEQLKSGDLILLNGDLGAGKTTLTQFI
                     GKALGVRRTINSPTFNIIKSYRGKNLKLHHMDCYRLEDSDEDLGFDEFFEDQAITVIE
                     WSQFIKDLLPATHLSINISTISENTRQIELFAQGEHYEQIKEAIIHEFAAH"
     gene            642..1304
                     /gene="tsaB"
                     /locus_tag="O2701_002593"
     CDS             642..1304
                     /gene="tsaB"
                     /locus_tag="O2701_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCZ4294458.1"
                     /translation="MNSLLIDTSNQPLSVALMQNDEVLAEITTDSKQNHSVQLMPAIS
                     QLFEQSKIAKQQLDAIIVAEGPGSYTGLRIGVTVAKTLAYALDVKLYGVSSLKALAAT
                     IDHTDKLLVPVFDARRQAVYTGIFQWQNGQLVTILEDQYMSIEALRAFLEDLNQPFIY
                     IGKDTVKLQDELQGEVIAQLPNASVMYQLLDKPSDVHTFTPKYHKLAEAERNWINSQK
                     NN"
     gene            1277..1741
                     /gene="rimI"
                     /locus_tag="O2701_002594"
     CDS             1277..1741
                     /gene="rimI"
                     /locus_tag="O2701_002594"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCZ4294459.1"
                     /translation="MDQQSKEQLNIREMTKEDVPQVFDLERRSFNDSSWTIDAFYHEI
                     EQNNFAKYFVLEFEQQIIGYLGLWIVIDQAQITTVAIDDNYRGYGLGQMLLKYGINYA
                     SHTCDVMSLEVRVNNKVAQHVYENLGFQYGGKRKNYYGEGEDAMVMWVNLND"
     gene            1734..2759
                     /gene="tsaD"
                     /locus_tag="O2701_002595"
     CDS             1734..2759
                     /gene="tsaD"
                     /locus_tag="O2701_002595"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCZ4294460.1"
                     /translation="MTKDILILAVETSCDETSVSVIKNGRDILSNTVLSQIESHKRFG
                     GVVPEVASRHHVEGITATINEALGDADVSIEDIDAIAVTEGPGLIGALLIGVNAAKAL
                     AFAYDKPLIPVHHIAGHIYANHIEEPLTFPLIALIVSGGHTELVYMKDHLSFEVIGET
                     RDDAVGEAYDKVARTIGLNYPGGPQVDRLAAEGEDTYSFPRVWLDKDSYDFSFSGLKS
                     AVINQLHNQRQKNIPIIEANVATSFQNSVVEVLTFKAIQACKEYGVQRLIVAGGVASN
                     KGLRQSLADQCKVNDIQLTIPSPKLCTDNAAMIGVAGHYLYQQGRFADLALNGHSNID
                     LEEYSAE"
     gene            complement(2861..4471)
                     /locus_tag="O2701_002596"
     CDS             complement(2861..4471)
                     /locus_tag="O2701_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MutS family DNA mismatch repair protein"
                     /protein_id="HCZ4294461.1"
                     /translation="MSTNQTFLIFVIAIILLTSVIGIVGRYMSRQRLLKSMETLWQTI
                     SPLEAFIRPNSHFDYEYKLYKEKFESHSLVDDKTWSDLNMNAIFHKMNYNLTAIGEMK
                     LYACLRGMLSITNKSLLSLFNDNAEFRKNVTYHLALIGKTVYPTFPDQITPVKRQNIL
                     FLCPFLPVISFAVIFINSQVGILLFLMSCLFNIILSATLKRTYEDDLKSIFYASNVLK
                     QGYTISKIKHAPQPEVNFKQFRTARHLTSVLAEVNDEDIGAMVIKLVKLIFMLDYVLF
                     HSIQKSYTTHMNELKNCFDYIAELDNHYALAMYRRTLECYTEPQIDDSNDGIVFSELT
                     HPLIADAVANDFSLSQNILLTGSNASGKSTFMKSIAINIILASAIQTVTASKFVYQPG
                     IVFTSMANADDVLSGDSYFMAELKSIKRIVEIPDNQKIYCFIDEIFKGTNTTERIAAS
                     ESVLSFLHEKSNFRVIAATHDIELAELLKQRYENYHFNEVIENNNIHFDYKIKPGKAN
                     TRNAIELLKITSFPAKIYERAKDNVPKI"
BASE COUNT         1533 a          630 c          827 g         1481 t
ORIGIN      
        1 aatttactat atctgcttta gtatgtcaac aaatatttta aattttctta caattaactt
       61 tgaatagata atctgtttca aagataagaa atttaagttg aaatttgtta caatataaag
      121 gttaaatata aagttagata cgattgaaca atggataatc atttttatga atgaaaagca
      181 taatatagga gaatcaacat tgataaagat aaataattta gatgaaatga atcaatttgc
      241 tatattttta gttgagcaat tgaaaagtgg tgatttgatt ttacttaacg gagatttagg
      301 agcaggtaaa acaacgttaa cgcaatttat aggaaaagct cttggtgtaa gacgtacgat
      361 taattccccg acatttaaca tcattaaatc atataggggt aaaaatttaa aattgcatca
      421 tatggattgt tatcgcttag aagattctga tgaagattta ggatttgatg aatttttcga
      481 agatcaggca attactgtta ttgaatggag tcaatttata aaagatttac ttccagcgac
      541 gcatttatct attaacattt caacaatatc tgaaaataca agacaaattg agttgttcgc
      601 gcaaggagaa cattatgaac aaattaagga ggcaattatc catgaattcg ctgctcattg
      661 atacatcgaa tcaaccatta tcagtagcat tgatgcaaaa cgatgaagtt ttagctgaaa
      721 taactacgga ttctaaacaa aatcattcag tgcaattaat gcctgcgatt agtcagctat
      781 ttgagcagag caaaatagct aaacagcaat tagatgcgat tattgttgct gaaggtcctg
      841 gttcatatac tggcttacgt atcggtgtta ctgttgctaa aacattagcg tatgcgttag
      901 atgttaaatt atatggtgtt tcatcattaa aggcattggc agcaacaatc gatcatactg
      961 ataaattatt ggttcctgtg tttgatgcaa gacgacaagc agtttataca ggcatattcc
     1021 agtggcaaaa tggtcaatta gtgactattt tagaagatca atatatgtcg atagaggcat
     1081 tgcgagcttt tttagaagat ttaaaccaac catttattta tattgggaaa gatactgtaa
     1141 aattacaaga tgaattacaa ggtgaagtga ttgcgcagct accaaatgca tcagtcatgt
     1201 atcaattgct agataaacct tcagacgtac atacatttac tcctaaatac cataaattag
     1261 ctgaggcgga acgaaattgg atcaacagtc aaaagaacaa ttaaacatta gagagatgac
     1321 taaagaagat gtgccacaag tctttgatct tgagcgtcgg agtttcaatg acagttcttg
     1381 gacaattgac gcattttatc atgaaataga gcaaaataac tttgcgaaat attttgtctt
     1441 agaatttgaa caacaaatta ttggttattt aggtttatgg attgttatcg atcaagctca
     1501 aattacaaca gttgcaatcg atgataatta tagaggctat ggtttaggtc aaatgctttt
     1561 aaaatacgga ataaactatg caagtcacac ttgtgatgtg atgagtttag aagtaagagt
     1621 taataataaa gtggcacaac atgtatatga aaatttagga tttcaatatg gtggtaaacg
     1681 taaaaattat tatggtgaag gtgaggacgc aatggttatg tgggtgaatt taaatgacta
     1741 aagatatatt aatactagct gttgaaacaa gttgtgatga aacaagcgtt agtgttataa
     1801 aaaatggcag agatatttta tcaaatacag ttttaagtca gattgaaagt cataaacgat
     1861 ttggcggtgt cgttcccgaa gtggcaagta gacatcacgt tgaaggtata acagcaacaa
     1921 taaacgaggc tctaggggat gccgatgtat caatagaaga tattgatgcc atagcggtta
     1981 cagaaggccc tggactaatt ggtgcgttac taataggtgt taatgcagcc aaagcattgg
     2041 catttgctta cgataagcca cttattcctg ttcatcatat tgcaggacat atatatgcta
     2101 atcacataga agagccatta acattcccgc taattgcact tattgtttca ggtggacata
     2161 ctgaattagt ttatatgaag gatcatttat catttgaagt cattggtgaa acacgagatg
     2221 acgcagtagg tgaggcttat gataaagtgg cacgaacaat tggtttaaat tatccaggtg
     2281 gtccacaagt tgatcggttg gctgctgaag gtgaagatac ttattcattc cctcgtgttt
     2341 ggttggataa agatagttat gattttagtt ttagtgggtt gaaaagtgcc gtaatcaatc
     2401 aacttcacaa tcaacgacaa aaaaatattc caatcattga agctaacgta gcaacgagct
     2461 ttcaaaacag tgttgtagag gtgctcacgt ttaaagctat tcaagcttgt aaagaatatg
     2521 gtgttcagcg attaattgtt gctggtggcg tggcgagtaa taaaggatta cgtcaatctt
     2581 tagcggatca atgcaaagtc aatgacattc aattaactat cccaagtcct aaattatgca
     2641 cagataatgc tgcaatgata ggcgttgccg gccactattt gtatcagcaa ggtcgatttg
     2701 ctgatttagc attaaatggg cacagcaata tagatttaga agagtattct gcagaataaa
     2761 ataaacgtca gttattaaca tgattactat gaacattatt gaaactgttc atagtcttct
     2821 gtcatgtgat agctgacgtt tttatgttta aagttaaatg ctaaattttc gggacattat
     2881 cttttgctcg ttcatatatt tttgctggaa atgaagtgat ttttaataat tcgatggcat
     2941 tacgtgtatt tgctttgcca ggcttaattt tgtaatcaaa atgtatgtta ttattttcta
     3001 ttacctcatt gaaatggtaa ttttcataac gttgttttaa gagttcagct aactcaatat
     3061 catgtgttgc tgcaataact ctaaagttag atttttcatg taaaaatgat agtactgatt
     3121 ctgaagcggc aattcgttca gttgtgttgg tacctttaaa aatttcatct ataaagcagt
     3181 atattttttg attatctgga atttcaacaa tacgcttaat agattttaat tcagccataa
     3241 aataactatc gcctgataac acatcatcgg catttgccat tgacgtgaac acaattccag
     3301 gttgatatac aaatttgctt gcggtaactg tttgtatagc cgatgcaaga ataatgttta
     3361 ttgctatgga tttcataaat gttgatttac ccgaagcatt agacccagtt aacagtatat
     3421 tttgtgatag tgagaaatca ttggctactg catctgctat gagtggatgt gttaactcag
     3481 agaatactat gccatcattt gaatcatcaa tttgtggttc agtataacat tctagcgttc
     3541 tacgatacat tgctaatgca taatgattat ctaattcagc tatgtaatcg aaacaatttt
     3601 taagctcatt catatgtgtt gtatagcttt tttgaattga atgaaataaa acataatcaa
     3661 gcatgaaaat aagtttaact agtttaatta ccatagctcc gatatcttca tcattaactt
     3721 cagctaaaac actcgttaaa tgacgtgctg ttctaaattg cttaaaattg acttcaggtt
     3781 gtggagcatg tttaattttt gagatagtat agccttgttt taaaacattg gatgcataaa
     3841 agatagattt taagtcatcc tcatatgtac gctttaaagt tgctgataaa atgatattaa
     3901 ataggcaact cataagaaat aataaaatac caacttgtga attaataaat ataactgcaa
     3961 atgagataac tggtaaaaac gggcatagaa acaatatatt ttgacgtttt accggtgtga
     4021 tttggtctgg aaatgttgga taaacagttt taccaatcaa agctaaatga tatgttacgt
     4081 tttttctaaa ttcagcatta tcattaaata aactaagtaa tgatttgttc gtaattgaaa
     4141 gcattccacg taaacaggca tatagcttca tttcaccaat agctgttaaa ttataattca
     4201 tcttatgaaa gattgcattc atatttaagt cggaccaagt tttatcatca actaatgaat
     4261 gtgattcaaa tttttccttg tagagcttat actcatagtc gaaatgtgag ttcggtctga
     4321 taaaagcttc taatggagaa atcgtttgcc ataatgtttc catagatttt aatagacgtt
     4381 gacgactcat gtatcgtcca acaattccta ttacagatgt aagtagaata attgctataa
     4441 caaatattaa aaatgtttga tttgtgctca t
//
LOCUS       DALAGD010000146         4459 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.151, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000146 DALAGD010000000
VERSION     DALAGD010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.151"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            55..4152
                     /gene="sdrD"
                     /locus_tag="O2701_002597"
     CDS             55..4152
                     /gene="sdrD"
                     /locus_tag="O2701_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrD"
                     /protein_id="HCZ4294462.1"
                     /translation="MLNRENKTAITRKGMVSNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAESTNKELNEATTSASDNQSSDKVDMQQLNQEDNTKNDNQKEMVSSQGNE
                     TTSNGNKLIEKESVQSTTGNKVEVSTAKSDEQASPKSTNEDLNTKQTISNQEALQPDL
                     QENKSVVNVQPTNEENKKVDAKTESTTLNVKSDAIKSNDETLVDNNSNSNNENNADII
                     LPKSTAPKRLNTRMRIAAVQPSSTEAKNVNDLITSNTTLTVVDADKNNKIVPAQDYLS
                     LKSQITVDDKVKSGDYFTIKYSDTVQVYGLNPEDIKNIGDIKDPNNGETIATAKHDTA
                     NNLITYTFTDYVDRFNSVQMGINYSIYMDADTIPVSKNDVEFNVTIGNTTTKTTANIQ
                     YPDYVVNEKNSIGSAFTETVSHVGNKENPGYYKQTIYVNPSENSLTNAKLKVQAYHSS
                     YPNNIGQINKDVTDIKIYQVPKGYTLNKGYDVNTKELTDVTNQYLQKITYGDNNSAVI
                     DFGNADSAYVVMVNTKFQYTNSESPTLVQMATLSSTGNKSVSTGNALGFTNNQSGGAG
                     QEVYKIGNYVWEDTNKNGVQELGEKGVGNVTVTVFDNNTNTKVGEAVTKEDGSYLIPN
                     LPNGDYRVEFSNLPKGYEVTPSKQGNNEELDSNGLSSVITVNGKDNLSADLGIYKPKY
                     NLGDYVWEDTNKNGIQDQDEKGISGVTVTLKDENGNVLKTVTTDADGKYKFTDLDNGN
                     YKVEFTTPEGYTPTTVTSGSDIEKDSNGLTTTGVINGADNMTLDSGFYKTPKYNLGNY
                     VWEDTNKDGKQDSTEKGISGVTVTLKNENGEVLQTTKTDKDGKYQFTGLENGTYKVEF
                     ETPSGYTPTQVGSGTDEGIDSNGTSTTGVIKDKDNDTIDSGFYKPTYNLGDYVWEDTN
                     KNGVQDKDEKGISGVTVTLKDENDKVLKTVTTDENGKYQFTDLNNGTYKVEFETPSGY
                     TPTSVTSGNDTEKDSNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQ
                     DSTEKGIKDVKVTLLNEKGEVIGTTKTDENGKYCFDNLDSGKYKVIFEKPAGLTQTGT
                     NTTEDDKDADGGEVDVTITDHDDFTLDNGYYEEETSDSDSDSDSDSDSDRDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDAGKHTPVKPMSTTKD
                     HHNKAKALPETGNENSGSNNATLFGGLFAALGSLLLFGRRKKQNK"
BASE COUNT         1826 a          680 c          788 g         1165 t
ORIGIN      
        1 ttgtatttaa aacaattgta ttatttaaaa atttgatgaa ttaggagtaa tctaatgcta
       61 aacagagaaa ataaaacggc aataacaagg aaaggcatgg tatccaatcg attaaataaa
      121 ttttcgatta gaaagtacac agtgggaaca gcatcaattt tagtaggtac aacattaatt
      181 tttggtctgg ggaaccaaga agcaaaggct gcagaaagta ctaataaaga attgaacgaa
      241 gcgacaactt cagcaagtga taatcaatcg agtgataaag ttgatatgca gcaactaaat
      301 caagaagaca atactaaaaa tgataatcaa aaagaaatgg tatcatctca aggtaatgaa
      361 acgacttcaa atgggaataa attaatagaa aaagaaagtg tacaatctac cactggaaat
      421 aaagttgaag tttcaactgc caaatcagat gagcaagctt caccaaaatc tacgaatgaa
      481 gatttaaaca ctaaacaaac tataagtaat caagaagcgt tacaacctga tttgcaagag
      541 aataaatcag tggtaaatgt tcaaccaact aatgaggaaa acaaaaaggt agatgccaaa
      601 actgaatcaa ctacattaaa tgttaaaagt gatgctatca agagtaatga tgaaactctt
      661 gttgataaca atagtaattc aaataatgaa aataatgcag atatcatttt gccaaaaagt
      721 acagcaccta aacgtttgaa tacaagaatg cgtatagcag cagtacagcc atcatcaaca
      781 gaggctaaaa atgttaatga tttaatcaca tcaaatacaa cattaactgt cgttgatgca
      841 gataaaaaca ataaaatcgt accagcccaa gattatttat cattaaaatc acaaattaca
      901 gttgatgaca aagttaaatc aggtgattat ttcacaatta aatactcaga tacagtacaa
      961 gtatatggat tgaatccgga agatattaaa aatattggtg atattaaaga tccaaataat
     1021 ggtgaaacaa ttgcgactgc aaaacatgat actgcaaata atttaattac atatacattt
     1081 acagattatg ttgatcgatt taattctgta caaatgggaa ttaattattc aatttatatg
     1141 gatgctgata caattcctgt tagtaaaaac gatgttgagt ttaatgttac gataggtaat
     1201 actacaacaa aaacaactgc taacattcaa tatccagatt atgttgtaaa tgagaaaaat
     1261 tcaattggat cagcgttcac tgaaacagtt tcacatgttg gaaataaaga aaatccaggg
     1321 tactataaac aaacgattta tgtaaatcca tcggaaaatt ctttaacaaa tgccaaacta
     1381 aaagttcaag cttaccactc aagttatcct aataatatcg ggcaaataaa taaagatgta
     1441 acagatataa aaatatatca agttcctaaa ggttatacat taaataaagg atacgatgtg
     1501 aatactaaag agcttacaga tgtaacaaat caatacttgc agaaaattac atatggcgac
     1561 aacaatagcg ctgttattga ttttggaaat gcagattctg cttatgttgt aatggttaat
     1621 acaaaattcc aatatacaaa tagcgaaagc ccaacacttg ttcaaatggc tactttatct
     1681 tcaacaggta ataaatccgt ttctactggc aatgctttag gatttactaa taaccaaagt
     1741 ggcggagctg gtcaagaagt atataaaatt ggtaactacg tatgggaaga tactaataaa
     1801 aacggtgttc aagaattagg agaaaaaggc gttggcaatg taactgtaac tgtatttgat
     1861 aataatacaa atacaaaagt aggagaagca gttactaaag aagatgggtc atacttgatt
     1921 ccaaacttac ctaatggaga ttaccgtgta gaattttcaa acttaccaaa aggttatgaa
     1981 gtaacccctt caaaacaagg taataacgaa gaattagatt caaacggctt atcttcagtt
     2041 attacagtta atggcaaaga taacttatct gcagacttag gtatttacaa acctaaatac
     2101 aacttaggtg actatgtctg ggaagataca aataaaaatg gtatccaaga ccaagatgaa
     2161 aaaggtatat ctggcgtaac ggtaacatta aaagatgaaa acggtaacgt gttaaaaaca
     2221 gttacaacag acgctgatgg caaatataaa tttactgatt tagataatgg taattataaa
     2281 gttgaattta ctacaccaga aggctataca ccgactacag taacatctgg tagcgacatt
     2341 gaaaaagact ctaatggttt aacaacaaca ggtgttatta atggtgctga taacatgaca
     2401 ttagatagtg gattctacaa aacaccaaaa tataatttag gtaattatgt atgggaagat
     2461 acaaataaag atggtaagca ggattcaact gaaaaaggta tttcaggcgt aacagttaca
     2521 ttgaaaaatg aaaacggtga agttttacaa acaactaaaa cagataaaga tggtaaatat
     2581 caatttactg gattagaaaa tggaacttat aaagttgaat tcgaaacacc atcaggttac
     2641 acaccaacac aagtaggttc aggaactgat gaaggtatag attcaaatgg tacatcaaca
     2701 acaggtgtca ttaaagataa agataacgat actattgact ctggtttcta caaaccgact
     2761 tacaacttag gtgactatgt atgggaagat acaaataaaa acggtgttca agataaagat
     2821 gaaaagggca tttcaggtgt aacagttacg ttaaaagatg aaaacgacaa agttttaaaa
     2881 acagttacaa cagatgaaaa tggtaaatat caattcactg atttaaacaa tggaacttat
     2941 aaagttgaat tcgagacacc atcaggttat acaccaactt cagtaacttc tggaaatgat
     3001 actgaaaaag attctaatgg tttaacaaca acaggtgtca ttaaagatgc agataacatg
     3061 acattagaca gtggtttcta taaaacacca aaatatagtt taggtgatta tgtttggtac
     3121 gacagtaata aagacggcaa acaagattca actgaaaaag gtatcaaaga tgttaaagtt
     3181 actttattaa atgaaaaagg cgaagtaatt ggaacaacta aaacagatga aaatggtaaa
     3241 tactgctttg ataatttaga tagcggtaaa tacaaagtta tttttgaaaa gcctgctggc
     3301 ttaacacaaa caggtacaaa tacaactgaa gatgataaag atgcagatgg tggcgaagtt
     3361 gacgtaacaa ttacggatca tgatgatttc acacttgata atggatacta cgaagaagaa
     3421 acatcagata gcgactcaga ttcggacagc gactcagatt cagacagaga ctcagactca
     3481 gatagtgatt cagactcgga tagcgattca gattcagaca gcgattcaga ttcagatagc
     3541 gattcagatt cagacagcga ctcagattca gacagcgact cagactcaga tagtgattca
     3601 gactcagata gcgactcaga ttcagacagc gactcagact cagacagcga ctcagactca
     3661 gatagtgact cagattcaga tagcgactca gattcggaca gcgattcaga ctcagatagc
     3721 gactcagatt cagatagcga ttcggactca gatagcgact cagattcaga tagtgattca
     3781 gactcagata gcgactcaga ttcagacagc gactcagatt cggatagcga ctcagattca
     3841 gacagcgact cagactcgga tagtgattca gactcagata gcgactcaga ctcagatagc
     3901 gattcagatt cagatagcga ctcagactca gacagcgatt cagactcaga cagcgactca
     3961 gactcagatg caggtaagca cacacctgtt aaaccaatga gtactactaa agaccatcac
     4021 aataaagcaa aagcattacc agaaacaggt aatgaaaata gcggctcaaa taacgcaacg
     4081 ttatttggcg gattattcgc agcattagga tcattattgt tattcggtcg tcgtaaaaaa
     4141 caaaataaat aatacagctt ataccaggtc cgtgaggcct ggttttttat ttcaaaattt
     4201 atgaaatgta tcttatgttt gagattaaat agtgcaattt tttaaaaaat ataacataaa
     4261 tcaacataaa tttaattcat ctttacatta aaagtacaaa cgaataagaa aagcaattaa
     4321 acacagtatc aaaccattta aaacaattga tataaaaaac aaacgaataa ttaaaaagta
     4381 ctagaattaa tttattcaaa ccaaagtgta aaataacagt cttagataaa taaatttatt
     4441 taaagtattg tgctttatc
//
LOCUS       DALAGD010000147         4410 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.152, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000147 DALAGD010000000
VERSION     DALAGD010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.152"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..368
                     /locus_tag="O2701_002598"
     CDS             <1..368
                     /locus_tag="O2701_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016912861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase family
                     protein"
                     /protein_id="HCZ4294463.1"
                     /translation="LWNRDVGNIYTGSLYLSLISLLQNHTFQPEEKVCLFSYGSGAVG
                     EIFSGSIVKGYDKALDKEKHLNMLESREQLSVEEYETFFNRFDNQEFDFERELTQDPY
                     SKVYLYSIEDHIRTYKIEK"
     gene            1205..1948
                     /locus_tag="O2701_002599"
     CDS             1205..1948
                     /locus_tag="O2701_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase"
                     /protein_id="HCZ4294464.1"
                     /translation="MLTVYGHRGLPSKAPENTIASFKAASEVEGINWLELDVAITKDE
                     QLIIIHDDYLERTTNMSGEITELNYDEIKDASAGSWFGEKFKDEHFPTFDDVVKIANE
                     YNMNLNVELKGITGPNGLALSKSMVKQVEEQLTNLNQNQEVLISSFNVVLVKLAEEIM
                     PQYNRAVIFHTTSFREDWRTLLDYCNAKIVNTEDAKLTKAKVKMVKEAGYELNVWTVN
                     KPARANQLANWGVDGIFTDNADKMVHLSQ"
     gene            2045..2473
                     /locus_tag="O2701_002600"
     CDS             2045..2473
                     /locus_tag="O2701_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HCZ4294465.1"
                     /translation="MKYDDFIVGETFKTKSLHITEEEIIQFATTFDPQYMHIDKEKAE
                     QSRFKGIIASGMHTLSISFKLWVEEGKYGEEVVAGTQMNNVKFIKPVYPGNTLYVIAE
                     ITNKKSIKKENGLVTVSLSTYNENEEIVFKGEVTALINNS"
     gene            complement(2519..>4410)
                     /gene="mec"
                     /locus_tag="O2701_002601"
     CDS             complement(2519..>4410)
                     /gene="mec"
                     /locus_tag="O2701_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PBP2a family beta-lactam-resistant peptidoglycan
                     transpeptidase"
                     /protein_id="HCZ4294466.1"
                     /translation="KNFKQVYKDSSYISKSDNGEVEMTERPIKIYNSLGVKDINIQDR
                     KIKKVSKNKKRVDAQYKIKTNYGNIDRNVQFNFVKEDGMWKLDWDHSVIIPGMQKDQS
                     IHIENLKSERGKILDRNNVELANTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQ
                     QMDQNWVQDDTFVPLKTVKKMDEYLSDFAKKFHLTTNETESRNYPLGKATSHLLGYVG
                     PINSEELKQKEYKGYKDDAVIGKKGLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIE
                     KKKKDGKDIQLTIDAKVQKSIYNNMKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMY
                     GMSNEEYNKLTEDKKEPLLNKFQITTSPGSTQKILTAMIGLNNKTLDDKTSYKIDGKG
                     WQKDKSWGGYNVTRYEVVNGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVG
                     EDIPSDYPFYNAQISNKNLDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAP
                     HLLKDTKNKVWKKNIISKENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKM
                     KQGETGRQIGWFISYDKDNPNMMMAINVKDVQDKGMASYNAKISGKVYDELYENGNKK
                     YDIDE"
BASE COUNT         1479 a          660 c          733 g         1538 t
ORIGIN      
        1 tattatggaa tagagatgtt ggtaacattt atacaggatc attatactta agtttaattt
       61 cgttattaca gaaccacaca ttccaaccag aagagaaagt atgtctattt agttatggtt
      121 caggagcagt aggagaaatc tttagtggtt caatcgttaa aggatatgac aaagcattag
      181 ataaagagaa acacttaaat atgctagaat ctagagagca attatcagtc gaagaatacg
      241 aaacattctt taacagattt gataatcaag aatttgattt cgaacgtgaa ttgacacaag
      301 atccatattc aaaagtatac ttatacagta tagaagacca tatcagaaca tataagatag
      361 agaaataaac tagtggccga ttgtgcttga tgagcttggg acataaatcc taactcgaaa
      421 taaataagca tatcactaaa ctgatttttt aaagtttaca gtgatatgct tattttttta
      481 tcttacgatt ttgtacgtgc atgcttgcct aggggtatgg ctcgagccat tagtctctcg
      541 cacatactat tccctcaggc gtcagcactt acaaaatcgg ttgtaatttt catttttata
      601 cgcattctta ctgagattat actaataaga ggaatagtaa aagcaattct aagtaaaatt
      661 gcagataaga ggtttgttaa aagcagttct cagtaaaatt acagataaga ggtacgttaa
      721 aagcagttct aagtaaaatt gcagataaga ggtttgttaa aagcagttct aagtaaaatt
      781 gcagataaga ggtacgttaa aagcaattcc atgcaaaatt gctgataagg ggtaagttaa
      841 aagcagttct cagtaaaatt gcagataaga ggtacgttaa aagcagttct aggcaaaatt
      901 gcagataaga ggtgcgttaa aagcagttct cagtaaaatt gctgataagg ggtaagttaa
      961 aagcaatcct aagtaaaatt gcagataagg ggtacagaaa aactagactt gattacaaaa
     1021 tggagcttgg gacataaatg attttttaaa aatgagatga gacgtagatt aactccataa
     1081 tcaatacgaa tctatcgact tctttattta tgatattcat ctctttttaa tggaaataaa
     1141 agtgcgatta atgtgataat acagttacgt taattaaaaa aataaaaatg caaggagagg
     1201 taatatgcta actgtatatg gacatagagg attacctagt aaagctccgg aaaatacaat
     1261 tgcatcattt aaagctgctt cagaagtaga aggtataaac tggttggagt tagatgttgc
     1321 aattacaaaa gatgaacaac tgattatcat tcatgatgat tatttagaac ggactacaaa
     1381 tatgtccggg gaaataactg aattgaatta tgatgaaatt aaagatgctt ctgcaggatc
     1441 ttggtttggt gaaaaattca aagatgaaca tttcccaact ttcgatgatg tagtaaaaat
     1501 agcaaatgaa tataatatga atttaaatgt agaattaaaa ggtattactg gaccgaatgg
     1561 actagcactt tctaaaagta tggttaagca agtggaagaa caattaacaa acttaaatca
     1621 gaatcaagaa gtgctcattt caagctttaa tgttgtgctt gttaaacttg cagaagaaat
     1681 catgccacaa tataacagag cagttatatt ccatacaact tcgtttcgtg aagactggag
     1741 aacactttta gattactgta atgctaaaat agtaaacact gaagatgcca aacttactaa
     1801 agcaaaagta aaaatggtaa aagaagcggg ttatgaattg aacgtatgga ctgtaaacaa
     1861 accagcacgt gcaaaccaac ttgctaattg gggagttgat ggtatcttta cagacaatgc
     1921 agataaaatg gtgcatttgt ctcaatagaa agttagaggt gagtcttacg tttcagtgac
     1981 ggtagactta cctttaacat gttacatact aaaaaattaa tttgaataag aaagagagac
     2041 atatatgaaa tacgatgatt ttatagtagg agaaacattc aaaacaaaaa gccttcatat
     2101 tacagaagaa gaaattatcc aatttgcaac aacttttgat cctcaatata tgcatataga
     2161 taaagaaaaa gcagaacaaa gtagatttaa aggtatcatt gcatctggca tgcatacact
     2221 ttcaatatca tttaaattat gggtagaaga aggtaaatac ggagaagaag ttgtagcagg
     2281 aacacaaatg aataacgtta aatttattaa acctgtatac ccaggtaata cattgtacgt
     2341 tatcgctgaa attacaaata agaaatccat aaaaaaagaa aatggactcg ttacagtgtc
     2401 actttcaaca tacaatgaaa atgaagaaat tgtatttaag ggagaagtaa cagcacttat
     2461 taataattca taataaaaca gtgaagcaac catcgttacg gattgcttca ctgttttgtt
     2521 attcatctat atcgtatttt ttattaccgt tctcatatag ctcatcatac actttacctg
     2581 agattttggc attgtagcta gccattcctt tatcttgtac atctttaaca ttaatagcca
     2641 tcatcatgtt tggattatct ttatcatatg atataaacca cccaatttgt ctgccagttt
     2701 ctccttgttt cattttgagt tctgcagtac cggatttgcc aattaagttt gcataagatc
     2761 tataaatatc ttctttatgt gttttattta cgacttgttg cataccatca gttaatagat
     2821 tgatattttc tttggaaata atatttttct tccaaacttt gtttttcgtg tcttttaata
     2881 agtgaggtgc gttaatattg ccattatttt ctaatgcgct atagattgaa aggatctgta
     2941 ctgggttaat cagtatttca ccttgtccgt aacctgaatc agctaataat atttcattat
     3001 ctaaattttt gtttgaaatt tgagcattat aaaatggata atcacttggt atatcttcac
     3061 caacacctag ttttttcatg cctttttcaa atttcttact gcctaattcg agtgctactc
     3121 tagcaaagaa aatgttatct gatgattcta ttgcttgttt taagtcgata ttaccattta
     3181 ccacttcata tcttgtaacg ttgtaaccac cccaagattt atctttttgc caacctttac
     3241 catcgatttt ataacttgtt ttatcgtcta atgttttgtt atttaaccca atcattgctg
     3301 ttaatatttt ttgagttgaa cctggtgaag ttgtaatctg gaacttgttg agcagaggtt
     3361 cttttttatc ttcggttaat ttattatatt cttcgttact catgccatac ataaatggat
     3421 agacgtcata tgaaggtgtg cttacaagtg ctaataattc acctgtttga gggtggatag
     3481 cagtacctga gccataatca tttttcatgt tgttataaat actcttttga actttagcat
     3541 caatagttag ttgaatatct ttgccatctt ttttcttttt ctctattaat gtatgtgcga
     3601 ttgtattgct attatcgtca acgattgtga cacgatagcc atcttcatgt tggagctttt
     3661 tatcgtaaag tttttcgagt ccctttttac caataactgc atcatcttta tagcctttat
     3721 attctttttg ttttaattct tcagagttaa tgggaccaac ataacctaat agatgtgaag
     3781 tcgcttttcc tagaggatag ttacgacttt ctgtttcatt agttgtaaga tgaaattttt
     3841 ttgcgaaatc acttaaatat tcatccattt ttttaacggt tttaagtgga acgaaggtat
     3901 catcttgtac ccaattttga tccatttgtt gtttgatata gtcttcagaa atacttagtt
     3961 ctttagcgat tgctttataa tcttttttag atacattctt tggaacgatg cctatctcat
     4021 atgctgttcc tgtattggcc aattccacat tgtttcggtc taaaatttta ccacgttctg
     4081 attttaaatt ttcaatatgt atgctttggt ctttctgcat tcctggaata atgacgctat
     4141 gatcccaatc taacttccac ataccatctt ctttaacaaa attaaattga acgttgcgat
     4201 caatgttacc gtagtttgtt ttaattttat attgagcatc tactcgtttt ttatttttag
     4261 atactttttt tattttacga tcctgaatgt ttatatcttt aacgcctaaa ctattatata
     4321 tttttatcgg acgttcagtc atttctactt caccattatc gcttttagaa atataactgc
     4381 tatctttata aacttgtttg aaatttttat
//
LOCUS       DALAGD010000148         4370 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.153, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000148 DALAGD010000000
VERSION     DALAGD010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.153"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            134..751
                     /locus_tag="O2701_002602"
     CDS             134..751
                     /locus_tag="O2701_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HCZ4294467.1"
                     /translation="MVTAIIHGFILAIGLILPLGAQNVFIFNQGANQPKYRYVLPAII
                     TAGLSDSLLIIIAVVGVSIIIMSLPVLQAIIYIVGLIFLMYMAWTIWHDKPSTDGETQ
                     IMSPMKQVSFALSVSLLNPHAILDTIGVIGSSAALYSGSNKIAFTIACISVSWLWFFL
                     LAILGKMVGSIDKTGKLLTIINKISSIIIIIVALMILQKLIQLLF"
     gene            complement(824..1405)
                     /locus_tag="O2701_002603"
     CDS             complement(824..1405)
                     /locus_tag="O2701_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCZ4294468.1"
                     /translation="MAKTLYIMRHGQTLFNFKGLIQGFGDSPLTELGIAQAQKARSYY
                     ETKGINFDLYASSTQERASDTLENVAPNQSYQRFKGLKEWHFGIFEGESVYLFDNLYK
                     PEDLFGDRIVPFKGEARQQVEERIVKTLHDIMSQTKNNALVVSHGTIMGVFLRYCLKL
                     DEALKHNIGNCNILKFEYDNGTFKFVELIDPNL"
     gene            1630..1866
                     /locus_tag="O2701_002604"
     CDS             1630..1866
                     /locus_tag="O2701_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294469.1"
                     /translation="MDNHNQCNYVNPQNVSLDWECFIISKSEMLLDGVPNELINTWLD
                     KDIITPFSIRNDEINFKTKDIWDALIHHNWYYSN"
     gene            complement(1863..2066)
                     /locus_tag="O2701_002605"
     CDS             complement(1863..2066)
                     /locus_tag="O2701_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HCZ4294470.1"
                     /translation="MTFYNFIMGFQNDNTPFGILAEHVSEDKAFPRLEERHQVIRAYV
                     MSNYTDHQLIETTNRAISLYMAN"
     gene            2407..2667
                     /locus_tag="O2701_002606"
     CDS             2407..2667
                     /locus_tag="O2701_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294471.1"
                     /translation="MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICG
                     VILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPIFYSFIG"
     gene            2696..2884
                     /locus_tag="O2701_002607"
     CDS             2696..2884
                     /locus_tag="O2701_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294472.1"
                     /translation="MSLHFAILFWLALIFLVAATFILVLMKKTSKESKKESYLSFTVI
                     LYIFGFAILIYTFIFGVL"
     gene            3072..3641
                     /locus_tag="O2701_002608"
     CDS             3072..3641
                     /locus_tag="O2701_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294473.1"
                     /translation="MNYIKRTIILLILFVVVSPINSPKTIADNKYSEIQDDKFQLQPG
                     DIIVTKGPVMWGFFGHCSIAIDDKTILQIEGPGDKPTTQSFESFKYNYASGKNDWMKV
                     YRCSYPGAGKKAAEWVKKNYENTNHRYLVTLNLNSKKFTYCTKIIYQAYKFGVSEKSV
                     KSYGLHIISPYAIKDNFIDPYKLRLVKAY"
     gene            3729..4013
                     /locus_tag="O2701_002609"
     CDS             3729..4013
                     /locus_tag="O2701_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294474.1"
                     /translation="MNELTKEQKYTIAKFYKLYIERSNKGETETVANFFGDAKDAREN
                     YFCDRDYQDFLTNCQILIQNKYLTGEVLDDNIYNISILNKTFIEFEQNFG"
     gene            4075..4293
                     /locus_tag="O2701_002610"
     CDS             4075..4293
                     /locus_tag="O2701_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294475.1"
                     /translation="MSEWHINNQSTKPFLIQQAEKQLSIVKPLPNGSFQILTEINLEN
                     GNISNIDHSLLVSVDPKALEISIFDAVN"
BASE COUNT         1495 a          642 c          628 g         1605 t
ORIGIN      
        1 tttctcataa attcaaatca tataggttct atttttcata caactactct actattgagt
       61 agttgttttt tatttgatat gataacaata agttttttca gaatattcta tgttttaggg
      121 gtgtcaaaac ttaatggtaa ccgcaattat tcatggattt atcttagcga ttggtctaat
      181 tttaccttta ggtgcacaaa atgtatttat ttttaaccaa ggagctaatc aaccaaaata
      241 tagatatgta ttgcctgcaa taattacagc cgggttgtca gacagcttac ttattattat
      301 tgcagtggta ggagtatcta tcattattat gtctttacct gtacttcaag caattattta
      361 tatagttggt ttaattttct tgatgtatat ggcttggacc atttggcatg ataaaccctc
      421 aacagatgga gaaactcaaa ttatgtctcc aatgaaacaa gtaagttttg ctttatcagt
      481 ttcattactc aatccacatg ctattttaga tacaattgga gtaattggta gtagtgctgc
      541 attatatagt ggcagcaata aaattgcgtt tacaatcgct tgtattagcg tttcatggtt
      601 atggttcttt ttactcgcaa ttttaggaaa aatggttgga tcaattgata aaaccggaaa
      661 attattaaca ataatcaata aaatatcaag cataataatt attattgttg ctctgatgat
      721 attacaaaaa cttattcaat tattattcta aagcgttatg tagttgtatt ttaaaagcgt
      781 tatacaaaag acatacttgt gtctatgtat aacgctttat ttattataaa tttggatcaa
      841 ttaactcaac aaatttaaat gttccattgt catattcaaa tttcaggata ttacaattac
      901 cgatattatg ctttaatgct tcatctagtt taaggcaata tcttaaaaat actcccatta
      961 ttgttccatg actcacgact aatgcattat tctttgtttg agacataatg tcatgtaaag
     1021 ttttcacaat gcgttcttca acttgttgcc ttgcctctcc tttgaaagga acaattcgat
     1081 ctccgaataa gtcctcaggc ttgtatagat tatcaaatag atagactgac tcaccttcaa
     1141 atattccaaa atgccattcc ttcagtccct taaaacgttg atacgattgg ttaggtgcaa
     1201 cattttcaag tgtgtcactt gcgcgttctt gcgttgatga tgcatataaa tcgaagttta
     1261 tccccttagt ttcataataa ctacgtgcct tttgagcttg agcaattcca agttctgtta
     1321 gcggcgaatc tccaaatccc tgaattagtc ccttaaaatt aaacaaagtt tgtccgtggc
     1381 gcattatata taacgtttta gccaacaata acacctcttc aataaaaagt attgatagct
     1441 caaaatgtat tctcttttta ttaaaatgag taccaaatat attaaaccat tatttaaact
     1501 ttttcggtag cgaaaacctt tgcttttgat gtacattttt ttcataagta atgatgattt
     1561 tagtttaaaa gataatgtga cggggtaaaa cgcaatgaag agtacagaat tttaaaggag
     1621 tgagtgttta tggataatca taatcaatgt aattatgtga atccacaaaa tgtttcactt
     1681 gactgggaat gttttataat tagtaaaagt gaaatgttgt tagatggtgt accaaatgaa
     1741 cttatcaaca cttggttaga taaagacatt attacaccat tttcgataag aaatgatgaa
     1801 ataaacttta aaacaaaaga tatttgggat gcactaatac atcataattg gtactactca
     1861 aattaatttg ccatatataa gctaatagct ctatttgtag tttcaattaa ttgatgatct
     1921 gtgtaattag acatcacata tgctctaatt acttggtgtc tttcttctaa tcgagggaat
     1981 gctttatctt cactaacgtg ttcggccaat ataccaaatg gtgtgttatc attttgaaaa
     2041 cccatgatga aattgtaaaa cgtcataaga cttgccccct tcatttatta agcgctattt
     2101 aaagtgttat tattaatctt atcagaaaac tagtatttgt aaatgtgcaa aagtgatttt
     2161 gcacattttt ttgatatctg attctaaaaa tattaacgaa ttatacaact ttaagagttt
     2221 ttagtatgta aatattcttt tcatattaat acaatacacc cctatatatg ctcacttgtt
     2281 tgaattaata aactttagca ttctgcaaat aatattttaa aaataactat actttttatt
     2341 tacctattga aaaatagtgt tatcatattt attaagaagc tatttttata ctagtaggtg
     2401 tcagtcatga ataacatttt gttaaatgct atcaatatag ttattactac cacttttgtt
     2461 atctttaata ttttaatcac atataataaa gatttagatg atttatgttg gctcctgcct
     2521 ggtattatca tttgtggtgt gatactcatc gtatccttta ccattgcaat gataactaaa
     2581 aactggttaa gtgaaatatt attttttata aatatcgtac tcgttctcta ttacatttat
     2641 cctatttttt atagttttat aggttaataa ttacggtata taaggagggt ttcacatgtc
     2701 tttacatttt gcaattctgt tttggctagc attaattttc ttagttgccg ctacgtttat
     2761 actcgtatta atgaaaaaaa ctagcaaaga atctaaaaaa gagtcctatt taagtttcac
     2821 tgtcattctc tatatttttg gattcgctat attaatatac acatttatat ttggtgtgct
     2881 ataaatgaaa ttcattttat atccagtctg aaacgtctca atgatgtttc agactgtttt
     2941 ttattcttaa tagacatttc acactctcat actttaatat ttaagatatg ttttagtctt
     3001 acaagcttat taaagattac aattctaatt taagactatt tacatatatt tcttcaacaa
     3061 ggagctttta agtgaactat attaaacgaa cgataatttt acttatatta ttcgttgtcg
     3121 tatccccaat aaactcacca aaaacaattg cagataataa gtattcagaa attcaagatg
     3181 acaaattcca attacaacct ggtgatatta ttgttacaaa aggccctgtc atgtggggat
     3241 tttttggtca ttgtagtatc gcgattgatg ataaaacgat tttacaaatt gaggggcctg
     3301 gcgacaaacc aactacacaa tccttcgaat cttttaaata taattatgca agtggcaaaa
     3361 atgattggat gaaagtttat cgttgcagct atcctggtgc aggtaaaaaa gcggctgaat
     3421 gggttaagaa aaactatgag aatacaaatc atcgttatct agttacatta aatttgaaca
     3481 gcaaaaaatt tacgtattgt actaaaatta tctaccaagc ttataaattt ggtgtaagtg
     3541 aaaaatcagt taaaagttat ggactacata ttatttcacc ctatgcaatt aaagataatt
     3601 ttatagatcc atacaaacta aggcttgtta aagcttatta acacagtttc atcaaatagt
     3661 taaattagtt tgtgttttgc aataaattgg gtatagatta caaatgatat ttcaggaggc
     3721 ccaatatttt gaatgaacta actaaagaac aaaagtatac aatagctaaa ttttacaaat
     3781 tatatattga acgttcgaat aaaggagaaa cagaaacagt cgctaacttt tttggcgatg
     3841 ctaaagatgc acgtgaaaac tatttttgcg atcgtgatta tcaagacttc ttaactaact
     3901 gccaaatttt aattcaaaac aaatacttaa ctggtgaagt tttggacgat aacatttaca
     3961 atatttctat tttaaacaag acatttattg aattcgaaca aaattttggt tgaacatgaa
     4021 tgtctcaact ataaactttc taaccctatt ttaaagcagg agtgaaaaac atacatgagt
     4081 gaatggcata ttaataatca atctacaaaa ccttttttaa tacaacaagc ggaaaagcaa
     4141 ttgtctattg ttaaaccgtt gcctaatggt agcttccaaa tactcacaga aataaatttg
     4201 gagaatggca atattagtaa catcgatcat tctttacttg tttcagtaga ccccaaagca
     4261 ttagaaataa gtatatttga cgctgtaaat taaacgcaat atttttattt aattatttta
     4321 atgtttcacc ttttaaataa aaacaagatt actgaattat tatgagattt
//
LOCUS       DALAGD010000149         4225 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.154, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000149 DALAGD010000000
VERSION     DALAGD010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.154"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            226..999
                     /locus_tag="O2701_002611"
     CDS             226..999
                     /locus_tag="O2701_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCZ4294476.1"
                     /translation="MIHSKRLRLWLYLVLLAVFIGACGMKKEESSKDKQIKENFNKTL
                     SLYPTKNLEDFYDKEGFRDQEFEKGDKGTWIIHSKMTIETNGKNMESRGLVLYVDRNT
                     RTTKGEFIVRELWEDKKGYSRSKEKEYPVKMEHNKIIPTKPIADDKLRKEIENFKFFV
                     QYGDFKDINDYKDGDISYNPNVPSYSAKYQLSNDDYNVKQLRKRYDIPTKKAPKLLIK
                     GDGDLKGSSIGHKNLEFTFIENKEENIYFTDSINFKPTE"
     gene            1111..1895
                     /locus_tag="O2701_002612"
                     /pseudo
     CDS             1111..1895
                     /locus_tag="O2701_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501248.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
     gene            1892..3216
                     /locus_tag="O2701_002613"
                     /pseudo
     CDS             1892..3216
                     /locus_tag="O2701_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3216..3542
                     /locus_tag="O2701_002614"
     CDS             3216..3542
                     /locus_tag="O2701_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294477.1"
                     /translation="MFTTYKNINELENAYDEERKQLNDAFNQIDELRHQTRKKCEQMY
                     DHFLYLKHKMNYSEDAMIRMTRIIESFDRETNQRIRHHEMKLEDYKDELRREYLKQSD
                     RIEGDE"
     gene            3545..3832
                     /locus_tag="O2701_002615"
     CDS             3545..3832
                     /locus_tag="O2701_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294478.1"
                     /translation="MGLDFSGLPDLAVLEQMKEKEQISEVIAPEHVRMHHDHQNKLKS
                     DEKILLDQMVSHFKKFEDDFKNAAQGAWVKNATDELKDISNDLEKIQDIKV"
     gene            4149..4225
                     /locus_tag="O2701_002616"
                     /pseudo
     CDS             4149..4225
                     /locus_tag="O2701_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014533130.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Csa1 family protein"
BASE COUNT         1735 a          530 c          764 g         1196 t
ORIGIN      
        1 ttattttaaa tataagcgca atttatctta attaattagt tggttaggcc aataaaaatt
       61 ataaagatat ttgcgctttt taaataattt gtgatgttga tatgtgaaaa atacaactaa
      121 caattaaaat ccacgaggat ttaatcataa aatttcctct aatattatta atattggaat
      181 ttgaacttaa ctttgcatca aaaataaaaa tggggatgtg aaatgatgat tcactcaaaa
      241 aggttgaggc tctggctgta tttagtatta ttagctgttt ttataggggc ttgcggaatg
      301 aaaaaggaag aaagcagtaa agataaacaa attaaagaaa acttcaacaa aacgttgagc
      361 ctgtatccaa ctaaaaatct tgaagacttt tatgataagg aaggctttcg agatcaagag
      421 tttgaaaagg gcgacaaagg cacttggata attcactcta aaatgacaat agaaacaaat
      481 ggaaagaata tggaatccag aggattggtc ctgtatgtag accgcaatac cagaacgaca
      541 aaaggtgaat ttattgtgag ggaattatgg gaagataaaa aaggatactc acgtagtaaa
      601 gaaaaagaat atccagtaaa aatggaacat aataaaatta tcccaaccaa accgatagct
      661 gatgataaat taagaaaaga aatcgaaaac tttaaattct ttgtacaata cggtgacttt
      721 aaagatatta atgattataa agatggtgac atttcatata atccgaatgt gccaagttat
      781 tcagcaaagt atcaattaag taatgatgac tacaatgtga agcaacttag aaagaggtat
      841 gatataccaa ctaagaaagc acctaaatta ttaataaaag gcgatggtga tttaaaagga
      901 tcatctatag gacacaaaaa tttagagttt acatttatag aaaataaaga agaaaacatc
      961 tattttacgg atagtattaa tttcaaacct actgaatagg gttaatagaa gtttgaaatg
     1021 aaaatttcat aaagtgttca aaataatttt tcgctttagt tgtgtaaatt aaagattgag
     1081 tcagtattaa aaatataagg tggagaaatg atgattcatt caaagaaatt aacgctcggt
     1141 atatgtttgg tattactcat tatattgatt ggaggttgtg tcattatgac aaaaacaaat
     1201 ggtcgaaacg ctcaaattaa agaaaatttc aacaaaacat taagtgtata tccaaccaag
     1261 aatctcgatg atttttacga taaggaaggt tttcgagatc aagaatttga taaaagagat
     1321 aaagggactt ggattattta ttctgaaatg gttatcgaac caaaagggaa aaatatggaa
     1381 tcgagaggaa tggtgctcta tatcaatcgc aatactagaa caacgaaggg taattttatt
     1441 gtcaccgaaa taactgaaga tagtaaagga tattcacgta gtaaagaaaa aaatatcctg
     1501 tcaagatgga aaataatcga attattccaa caaagcctat accggatgac aagttaaaaa
     1561 aagagattga aaactttaag ttctttgtac aatatggaaa ttttaaagat tttaaagatt
     1621 ataaaaatgg tgatatttca tataatccta atgtgccaag ttattctgca aagtatcaat
     1681 tgaataatga tgactataat gttcaacagt taagaaaacg atatcatatt ccaaccaaac
     1741 aagcgcccga attaaaattg aaaggatccg gcaatttaaa aggctcatcc gtaggatcta
     1801 aggatctaga atttacgttt gtagaaaatc aagaagagaa tatctatttt tcagattcgg
     1861 tcgaatttac acctagcgag gatgataaat catgagtcaa acggaatatc aaataaaatc
     1921 tggcaatata aaaggtaact ctgaagaaac aagtacagta tctaatataa gttatgaaat
     1981 agaaaacgca aataacagtg gtttaaaaca aaataaaatt gataaacaaa ttaaaaagtt
     2041 acaagaaaaa aataaattcc ctaaaaatct ttcatatctt aaaagttata cggaccccaa
     2101 aacaggcacg actacaagcg cctttttaaa taaagacact ggcaaagtta ctttaggtat
     2161 gacaggtact aatgtacaca aagacgcaat attaaaacaa acatttggtg ttccttctta
     2221 tcaaggatat atagatgtga gtgaaacgct aaaggatatt ggggccgatg tcaatattgg
     2281 ccttcattcc gtcacagata aagatccaca ttataaaaat acccaagact ttatcaaaaa
     2341 tatcaaaaaa gactatgata ttgatattat taccggacat tcgctgggcg gtagagatgc
     2401 gatgatttta ggtatgagta atgatattaa acatatcgtt gtgtataatc cagctccatt
     2461 agcgatcaaa gatgtgagcg gcctttatgc cgatcaagaa gaacttaaaa aattgattga
     2521 aaagtacgat ggtcacattg taagatttgt gtctgatgaa gacgaattag atgcaggtgt
     2581 ccgcaatcat ttatatgaaa ctgctggaga aaaaatagta cttaaaaatg gagaaggcca
     2641 tgcaatgagt ggtattttaa tgagcagaac acaggctata atcttagctg aattaaacaa
     2701 agttaaaggc taccaagacg aaaataataa agcattaaaa tccgttcgta aacaaacgag
     2761 gcatagatta cataaagtag agacgttaag agcgaattgg attcaaacaa cgggtggatc
     2821 actctcttcc tcccaacaac aattattaga agctttaaca gcactaacca ttgccgaagg
     2881 cttaaatcaa ttagtgaatg aagaaagcca acatttgaaa aaatgtatca cgcgatggca
     2941 cataaatttg gagacaactg gaaaaaagcg caagaagttg gaaatgaaat tggtgaaaaa
     3001 ttaacctctg aagaggttat agatgaatta agaaaaggtg gcgcgtatga aagtaaactt
     3061 gaaacagatc ccaaaagaaa aattgatgat aagataaaga aattaaatga tgtttataaa
     3121 aattgtaatg gctatatcgc aaaaattaaa cagagtatcg aagcaattgt ttctaatgac
     3181 caaatgttag cgagccagat tgatgggatg atgtaatgtt tactacgtat aaaaatatta
     3241 atgaacttga aaatgcctat gatgaagaaa gaaaacaatt gaatgatgca ttcaatcaaa
     3301 ttgatgaatt aagacatcaa acacgcaaga aatgtgaaca aatgtatgat catttcttat
     3361 atctcaaaca taaaatgaat tatagtgaag acgctatgat caggatgaca cgtattatag
     3421 aatctttcga tagagaaacg aatcaacgta tccgacatca cgaaatgaaa ttagaagatt
     3481 ataaagatga gttaagaaga gaatatctaa aacaatctga cagaattgaa ggagatgaat
     3541 aagcgtggga cttgatttta gtggtttacc agatttagca gtattggaac aaatgaagga
     3601 aaaagaacag attagtgagg ttattgcgcc tgaacatgtt cgtatgcatc atgatcatca
     3661 aaataagctg aaaagtgatg agaaaatatt acttgaccaa atggtaagtc atttcaaaaa
     3721 atttgaagat gattttaaaa atgcggcaca aggggcttgg gtgaaaaatg ccacagacga
     3781 attaaaagat attagtaatg atttagaaaa aattcaagat attaaagtat aaaaaggtat
     3841 taagaaacac aataagtata taatccattt aataataaat gaattatata gttcataatt
     3901 tcgactataa gtggctatta gccattattt ttcgggatct atgtcaaatc ggactaatga
     3961 attcaataat ggaagttaag caaccaatct ttgtttaact tcttttttat tttttggaaa
     4021 ataaagtttt gaacataata aaatttgatt atgttttaac gaattttgat gtttcttgaa
     4081 ctatatatca tctagtcgtc atttacaatt ggtaaatatg acttcaaact gtatgaaggt
     4141 gatggcgatt aaaaggctca tccgtaggtt ctaaagaact agaatttaac tttataagaa
     4201 ataaagaaga aaatgtgtat ttttc
//
LOCUS       DALAGD010000150         4210 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.155, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000150 DALAGD010000000
VERSION     DALAGD010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.155"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(<1..585)
                     /locus_tag="O2701_002617"
     CDS             complement(<1..585)
                     /locus_tag="O2701_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCZ4294479.1"
                     /translation="MSQNTNHSYYHQNQHAQSISKVWLYFMYYWIIFGIGCYLGQFLP
                     LSWRQPLSFGLLIIILATLVFERARRFGLIISHIYAVVIGLLSYATFTTYLQNLGPDI
                     FYKNIALAIFAFIAFGIIGYFFVGDASSIGKYLFVTLITLIIASLIGIFLQNPIFYTI
                     ITVVSLLLFLLYTLYDFNRLKRGDYSPREMGFNLF"
     gene            946..1743
                     /locus_tag="O2701_002618"
     CDS             946..1743
                     /locus_tag="O2701_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCZ4294480.1"
                     /translation="MKKLAFAITATSGAAAFLTHHDAQASTQHTVQSGESLWSIAQKY
                     NTSVESIKQNNQLDNNLVFPGQVISVGGSDAQNTSNTSPQAGSASSHTVQAGESLNII
                     ASRYGVSVDQLMAANNLRGYLIMPNQTLQIPNGGSGGTTPTATTGSNGNASSFNHQNL
                     YTAGQCTWYVFDRRAQAGSPISTYWSDAKYWAGNAANDGYQVNNTPSVGSIMQSTPGP
                     YGHVAYVERVNGDGSILISEMNYTYGPYNMNYRTIPASEVSSYAFIH"
     gene            complement(2331..3338)
                     /locus_tag="O2701_002619"
     CDS             complement(2331..3338)
                     /locus_tag="O2701_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HCZ4294481.1"
                     /translation="MSYIIIVTIAVVIFSLIFDFINGFHDTANAVATAVSTRALTPKT
                     AILMAAVMNFIGALTFTGVAGTITKDIVDPFKLENGLVVVLAAILAAIIWNLATWFYG
                     IPSSSSHALIGSIAGAAIASEGSFGVLHYQGFTKIIIVLIVSPIIAFCVGFLMYSIFK
                     VIFKNANLTRANRNFRFFQIFTAALQSFSHGTNDAQKSMGIITLALIVANVQNDGSVE
                     PQLWVKFACATAMGLGTAIGGWKIIKTVGGNIMKIRPANGAAADLSSALTIFVASSLH
                     FPLSTTHVVSSSILGVGASNRAKGVKWSTAQRMIITWVITLPISALLAGLLFYILNLF
                     F"
     gene            complement(3354..3971)
                     /locus_tag="O2701_002620"
     CDS             complement(3354..3971)
                     /locus_tag="O2701_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HCZ4294482.1"
                     /translation="MFSKKKDKFMVQLEEMVFNLDRAAIEFGKMDFNTHLDLKAYSDN
                     IKTYESHGDELVHQVITDLNQTFITPIEREDILSLCDAIDDVLDAIEETAAMFEMYSI
                     EYTDEYMAEFVDNIQKAVAEMKLAVGLLVDKKLSHMRIHSINIKEFETNCDGILRQSI
                     KHIFNSETDPITLIKIKDIYESMEEIADKCQIVANNFETIIMKNS"
BASE COUNT         1512 a          724 c          585 g         1389 t
ORIGIN      
        1 aaatagatta aatcccattt ctcttggtga atagtcacct ctttttaaac gattaaaatc
       61 atacaaagta taaagtagaa ataacaacag actaacgacg gtaataatag tgtaaaaaat
      121 aggattttga agaaaaatac caattagact cgcaataatt aatgttatta atgtaacgaa
      181 taaatatttg cctatactcg atgcatctcc aacgaagaaa taaccaataa taccaaatgc
      241 tataaatgca aaaattgcta atgcgatatt tttatagaaa atatctggtc ctaaattttg
      301 taaatacgtg gtaaacgttg cgtatgacaa taagccaatc actacagcgt aaatatgtga
      361 aataattaaa ccgaaccgtc tcgctctttc aaaaacaagt gttgctaaaa taataatcag
      421 taatccaaat gacaagggtt gtcgccaact taatggtaaa aactgaccta gatagcatcc
      481 tatgccaaat ataatccaat aatacataaa ataaagccac actttactta ttgattgagc
      541 atgctggttt tgatgataat atgaatgatt tgtattttgc gacaaaataa ttcctccttc
      601 taccgtcgtt ttatgcgtca aattttacag taaattttgc ttcatcaaaa gaaataacct
      661 ttaacaagta tagtaatttt acattttaca atgttacaaa atataatttt ttataattag
      721 ttaaaatcac taaaacgctt ttatacacta tcaaatcagc atttataaaa atatgaaccg
      781 atatcctaaa atgttaataa tattacaaga taataacaaa ccacacaaag ctacttattt
      841 ttgataatat ggaaatcgta atataaaacg aaaacttaat ttactatata aattgtctta
      901 ataattttta aaagtagtaa aacataattt taaggaggag tccctttgaa aaaattagca
      961 tttgcaataa cagcaacatc tggtgcagct gcatttttaa cgcatcatga tgcacaagct
     1021 tctacacaac atacagtaca atctggtgaa tcattatgga gtattgctca aaaatacaac
     1081 acttcagtag agagtattaa acaaaataac caattagata acaacttggt attccctggt
     1141 caagttatct cagtaggtgg aagtgatgca caaaatacgt caaacacttc tccacaagct
     1201 ggttcagcat catctcatac tgtacaagct ggtgaatcat taaatatcat tgctagcaga
     1261 tatggtgttt cagttgatca attaatggca gccaataact tacgtggtta tttaattatg
     1321 cctaaccaaa cattacaaat tcctaatggt ggatcaggtg gtacaacacc aacagctaca
     1381 acaggtagca atggcaatgc atcatctttt aatcaccaaa atttatacac tgctggtcaa
     1441 tgtacatggt acgtatttga ccgtcgtgct caagctggta gtccaattag cacatattgg
     1501 tcagacgcta agtattgggc tggtaacgca gctaatgatg gttaccaagt aaacaacaca
     1561 ccatcagttg gttcaattat gcaaagcaca cctggtccat atggtcatgt tgcttatgtt
     1621 gaacgtgtca atggtgatgg tagtatcttg atttctgaaa tgaattacac atatggtcca
     1681 tacaatatga actaccgtac aattccagct tcagaagttt ctagctatgc attcatccat
     1741 taattaaata aattgtactg atatatacta gcaattcaca tcatgtgaga ttgctagttt
     1801 tttatttttg aaaaaaattt tcattttggt acaaaaaatt atctcaccct tccctatcat
     1861 acatatttat attttgtatg aatggtagtt aggtaaaaat taacaaccta cctatttgat
     1921 taaattaaat gcataatata ttaaaaatca aaataatgca actaagttga ctatataatg
     1981 atttaatttg ttataccagt attttacgct ttttcgtcta catatacaaa tttatattaa
     2041 ataaagccca atacaattta ggttaattaa acaagttgat aactatttaa ttattccttc
     2101 attgaagaat ataaactatt aaatcattat tttgctctta catatatttt aatgacctaa
     2161 ctgattatgt tccatggaat acatttataa tatagcctcc taattaaaat gctttgtctt
     2221 ggtcattcta cgtaaattct ataaaatatg ttatctactt acataaaaaa ctgaacttca
     2281 ataccaccat atgtttgtga tactgaagtt cagtttagtt ttattttcaa ttagaaaaat
     2341 aagttaagta tatagaatag taaacctgct aacaatgctg aaataggtaa tgtaatcacc
     2401 catgtaatga tcattcgttg cgcagtgctc cattttacac ctttagctcg gttagaagca
     2461 ccaacaccta agattgatga tgacacaacg tgagttgttg ataatgggaa atgtagcgat
     2521 gatgcaacaa aaattgttaa tgcagatgat aaatcggccg cagcaccatt tgctggacgt
     2581 attttcataa tattaccacc tacagttttg ataattttcc agccaccaat tgcagtacca
     2641 agccccattg ctgtcgcaca ggcaaatttt acccataact gtggttcaac actgccatca
     2701 ttctgtacat tagcgacaat caatgccaac gtaataatac ccattgattt ttgcgcatca
     2761 ttcgtaccgt gagagaatga ttgtaacgct gctgtgaaaa tttggaaaaa tctaaagtta
     2821 cgattcgctc ttgttaaatt tgcattttta aagataactt taaaaattga atacatcaag
     2881 aaaccaacac aaaatgcgat aatcggtgaa acgattaata caataataat ttttgtgaaa
     2941 ccttggtaat gtaacactcc aaatgagcct tcagatgcga ttgctgcacc cgcaattgaa
     3001 cctataagtg catgtgaaga cgaacttgga attccgtaaa accaagtagc taaattccaa
     3061 ataatagccg caagtattgc agctaacaca acaactaatc cattttccaa tttaaatgga
     3121 tcgacaatgt ctttagtaat ggtgcctgca acgcccgtaa atgttaaagc acctataaag
     3181 ttcatcactg ctgccattaa aattgccgtt ttaggcgtta acgctctagt agatacagca
     3241 gtagctactg cattggctgt atcatggaat ccattgataa agtcaaatat cagcgagaaa
     3301 ataactacag ctatagtgac gatgattata tatgacataa atatatactc cccttagcta
     3361 tttttcataa taatagtttc aaaattattt gctacgattt gacatttatc agcgatttct
     3421 tccatgcttt cataaatatc ttttatttta attaaagtga ttggatctgt ttcgctattg
     3481 aaaatatgtt taattgactg tcttaaaata ccatcacagt ttgtttcaaa ttctttaata
     3541 ttaattgaat gaatacgcat atgtgataat tttttatcga ctaataagcc gacagcaagt
     3601 ttcatttctg caactgcttt ttgaatgtta tcaacaaact cagccatata ttcatctgtg
     3661 tattcgattg aatacatttc aaacatagct gccgtttctt caattgcatc taaaacatca
     3721 tcaattgcat cacataatga taaaatatct tcacgttcaa ttggtgtgat aaatgtttga
     3781 tttaaatcag taattacttg atgtactaat tcgtcaccat gtgactcata agttttaatg
     3841 ttgtctgagt atgcttttaa atctaaatgt gtattgaaat ccattttacc gaattcaata
     3901 gcagcacgat ccagattgaa aaccatctct tctaattgaa ccataaactt atcttttttc
     3961 ttactaaaca tttaaaatcc tccatttaag cgattgtcac caatcacatt cagttataat
     4021 ttgtttcaaa ttaagacaag tgaatttaca aactaatgat acaaatttgt tattatcaat
     4081 cgtcagtata attttagtgt actgatatta atttcaaaaa tgcctcacag taaacaattt
     4141 actgtatttg cccttatatg aaaatagcac aaaatttaca atcccacaat tcaatttaca
     4201 aatttttaac
//
LOCUS       DALAGD010000151         4107 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.156, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000151 DALAGD010000000
VERSION     DALAGD010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.156"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            58..492
                     /locus_tag="O2701_002621"
     CDS             58..492
                     /locus_tag="O2701_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HCZ4294483.1"
                     /translation="MKLKSFITVTLALGMIATTGATVAGNEVSAAEKDKLPATQKAKE
                     MQNVPYTIAVDGIMAFNQSYLNLPKDSQLSYLDLGNKVKALLYDERGVTPEKIRNAKS
                     AVYTITWKDGSKKEVDLKKDSYTANLFDSNSIKQIDINVKTK"
     gene            complement(749..934)
                     /locus_tag="O2701_002622"
     CDS             complement(749..934)
                     /locus_tag="O2701_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HCZ4294484.1"
                     /translation="MKHLVTFFWALLLMQMVNFVLNSLVGGDSLNVVNPIIMAVLFTI
                     FTAIFAAVIKPPKDSSQ"
     gene            1229..2170
                     /locus_tag="O2701_002623"
     CDS             1229..2170
                     /locus_tag="O2701_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HCZ4294485.1"
                     /translation="MNVGIKGFGAYAPEKIIDNAYFEQFLDTSDEWISKMTGIKERHW
                     ADDDQDTSDLAYEASLKAIADAGIQPEDIDMIIVATATGDMPFPTVANMLQERLGTGK
                     VASMDQLAACSGFMYSMITAKQYVQSGDYHNILVVGADKLSKITDLTDRSTAVLFGDG
                     AGAVIIGEVSDGRGIISYEMGSDGTGGKHLYLDKDTGKLKMNGREVFKFAVRIMGDAS
                     TRVVEKANLTSDDIDLFIPHQANIRIMESARERLGISKDKMSVSVNKYGNTSAASIPL
                     SIDQELKNGKIKDDDTIVLVGFGGGLTWGAMTIKWGK"
     gene            2182..3426
                     /gene="fabF"
                     /locus_tag="O2701_002624"
     CDS             2182..3426
                     /gene="fabF"
                     /locus_tag="O2701_002624"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCZ4294486.1"
                     /translation="MSQNKRVVITGMGALSPIGNDVKTTWENALKGVNGIDKITRIDT
                     EPYSVHLAGELKNFNIEDHIDKKEARRMDRFTQYAIVAAREAVKDAQLDINENTADRI
                     GVWIGSGIGGMETFEIAHKQLMDKGPRRVSPFFVPMLIPDMATGQVSIDLGAKGPNGA
                     TVTACATGTNSIGEAFKIVQRGDADAMITGGTEAPITHMAIAGFSASRALSTNDDIET
                     ACRPFQEGRDGFVMGEGAGILVIESLESAQARGANIYAEIVGYGTTGDAYHITAPAPE
                     GEGGSRAMQAAMDDAGIEPKDVQYLNAHGTSTPVGDLNEVKAIKNTFGEAAKHLKVNS
                     TKSMTGHLLGATGGIEAIFSALSIKDSKVAPTIHAVTPDPECDLDIVPNEAQDLDITY
                     AMSNSLGFGGHNAVLVFKKFEA"
     gene            complement(3481..3852)
                     /locus_tag="O2701_002625"
     CDS             complement(3481..3852)
                     /locus_tag="O2701_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HCZ4294487.1"
                     /translation="MLKKWLGMALITPILTFIIWLFNSHTIITYLNILFYVSLIIFIS
                     IFLILLVQEGIFDATSYGFRRLKYQMSSSKKKKSISDDPFFNPQEVKKEHYFVSTWII
                     PLLLINILYFIMTIVLSLILV"
BASE COUNT         1475 a          586 c          773 g         1273 t
ORIGIN      
        1 caatttgtgt tatattgatt ttgtaagata ctttaactca caaaggagag agattatatg
       61 aaattaaaat catttataac tgtaactttg gcactgggca tgatcgcaac gactggcgct
      121 actgtggcag gtaatgaggt atctgcagca gaaaaggaca aactaccggc aactcaaaaa
      181 gctaaagaaa tgcaaaatgt tccatataca attgcagtag atggcattat ggctttcaat
      241 caatcttact taaatttacc aaaagatagc caattatcat atttagattt aggaaataaa
      301 gttaaagctt tgttatatga tgaacgcggt gtaacacctg agaagattcg aaatgcaaaa
      361 tctgccgttt acacgattac ttggaaagat ggtagtaaaa aagaagtgga tcttaagaaa
      421 gatagctaca cagcaaactt gtttgattca aattcaatta aacaaattga tattaatgta
      481 aaaactaaat aaacacttaa aattatcaaa ttcaacatga agcatttaat tttacagtga
      541 tgattataaa ataattgcct tgatacaaag attactcgta aatgacatct ttgtattaag
      601 gctttttcta aatttaaaag tgatgggtta gaggtcattg agctttaaaa tattcaaaat
      661 acaaaacatt aatggccaaa aataaaagcc gcctttatct gggcagcttc aataataaga
      721 aagacatatt tcattttata ctaaatagtt attgtgatga atctttcggc ggtttaatta
      781 ctgcagcaaa aattgctgtg aaaatcgtga acaatactgc catgataatt ggattcacta
      841 catttaagct gtctccacct actaggctat taagtacaaa gttaaccatt tgcattaata
      901 ataatgccca aaagaatgtt acgaggtgtt tcatgtcatt ctacctccac tttaattata
      961 tatattttat tttaagtgaa agttagaaat ttgtatagta acatctcata tattttgacc
     1021 atattataca gtttaaataa atgattttat ctgaatggct attctaaatt aagcgcatta
     1081 aaaccaattt catactgaaa tttgacgata ataaagcatt aaaattttat taactagtca
     1141 atattcctac ctctgacttg agtttaaaaa gtaatctatg ttaaattaat acctggtatt
     1201 aaaaatttta ttaagaaggt gttcaactat gaacgtgggt attaaaggtt ttggtgcata
     1261 tgcgccagaa aagattattg acaatgccta ttttgagcaa tttttagata catctgatga
     1321 atggatttct aagatgactg gaattaaaga aagacattgg gcagatgatg atcaagatac
     1381 ttcagattta gcatatgaag caagtttaaa agcaatcgct gacgctggta ttcagcccga
     1441 agatatagat atgataattg ttgccacagc aactggagat atgccatttc caactgtcgc
     1501 aaatatgttg caagaacgtt tagggacggg caaagttgcc tctatggatc aacttgcagc
     1561 atgttctgga tttatgtatt caatgattac agctaaacaa tatgttcaat ctggagatta
     1621 tcataacatt ttagttgtcg gtgcagataa attatctaaa ataacagatt taactgaccg
     1681 ttctactgca gttctatttg gagatggtgc aggtgcggtt atcatcggtg aagtttcaga
     1741 tggcagaggt attataagtt atgaaatggg ttctgatggc acaggtggta aacatttata
     1801 tttagataaa gatactggta aactgaaaat gaatggtcga gaagtattta aatttgctgt
     1861 tagaattatg ggtgatgcat caacacgtgt agttgaaaaa gcgaatttaa catcagatga
     1921 tatagattta tttattcctc atcaagctaa tattagaatt atggaatcag ctagagaacg
     1981 cttaggtatt tcaaaagaca aaatgagtgt ttctgtaaat aaatatggaa atacttcagc
     2041 tgcgtcaata cctttaagta tcgatcaaga attaaaaaat ggtaaaatca aagatgatga
     2101 tacaattgtt cttgtcggat tcggtggcgg cctaacttgg ggcgcaatga caataaaatg
     2161 gggaaaatag gaggataacg aatgagtcaa aataaaagag tagttattac aggtatggga
     2221 gccctttctc caatcggtaa tgatgtcaaa acaacatggg agaatgctct aaaaggcgta
     2281 aatggtatcg ataaaattac acgtatcgat actgaacctt atagcgttca cttagcagga
     2341 gaacttaaaa actttaatat tgaagatcat atcgacaaaa aagaagcgcg tcgtatggat
     2401 agatttactc aatatgcaat tgtagcagct agagaggctg ttaaagatgc gcaattagat
     2461 atcaatgaaa atactgcaga tcgaatcggt gtatggattg gttctggtat cggtggtatg
     2521 gaaacatttg aaattgcaca taaacaatta atggataaag gcccaagacg tgtgagtcca
     2581 tttttcgtac caatgttaat tcctgatatg gcaactgggc aagtatcaat tgacttaggt
     2641 gcaaaaggac caaatggtgc aacagttaca gcatgtgcaa caggtacaaa ttcaatcgga
     2701 gaagcattta aaattgtgca acgcggtgat gcagatgcaa tgattactgg tggtacagaa
     2761 gcaccaatta ctcatatggc aattgctggt ttcagtgcaa gtcgagcgct ttctacaaat
     2821 gatgacattg aaacagcatg tcgtccattc caagaaggta gagatggttt tgttatgggt
     2881 gaaggtgctg gtattttagt aattgaatct ttagaatcag cacaagctcg aggtgccaat
     2941 atttatgctg agatagttgg ctatggtact acaggtgatg cttatcatat tacagcgcca
     3001 gctccagaag gtgaaggtgg ttctagagca atgcaagcag ctatggatga tgctggtatt
     3061 gaacctaaag atgtacaata cttaaatgcc catggtacaa gtactcctgt tggtgactta
     3121 aatgaagtta aagctattaa aaatacattt ggtgaagcag ctaaacactt aaaagttaac
     3181 tcaacaaaat caatgactgg tcacttactt ggtgcaacag gtggaattga agcaatcttc
     3241 tcagcgcttt caattaaaga ctctaaagtc gcaccgacaa ttcatgcggt aacaccagat
     3301 ccagaatgtg atttggatat tgttccaaat gaagcgcaag accttgatat tacttatgca
     3361 atgagtaata gcttaggatt cggtggacat aacgcagtat tagtattcaa gaaatttgaa
     3421 gcataactat aaaaatcttc agtaacgttg ttttagttac tgaagatttt ttcagtttct
     3481 ttatactaag atgagcgaca gcacaatcgt cataataaaa tataaaatat ttattaataa
     3541 taaggggatt atccatgtag aaacaaagta atgctctttt tttacctctt gtgggttgaa
     3601 aaatggatca tcagagatag acttcttctt tttcgaagat gacatttgat actttaatct
     3661 tctaaaacca taacttgtcg catcaaaaat gccttcttgt acaagtaaaa tcaaaaatat
     3721 gctaataaaa ataattaatg aaacataaaa caatatattt aaatatgtaa tgatagtatg
     3781 gctattaaaa agccatataa taaacgttaa tattggcgtt attagtgcca ttccaagcca
     3841 ttttttcaac atttgatcac tcccacttat agaaaactct tacgcatagt ttacattaaa
     3901 atcagacatt gaggaatgat tttttaattt cttcagcttt attgaaattc taaaatcaat
     3961 cattcttcat tagtttaaag caaaaaaata ttgatatata gtaaatattg tatatataat
     4021 attagttaag atttcagaaa attttgaagg gaatggaaat ttagaaatcg gaatttgtta
     4081 gaggagggga ttagatgggg aaatata
//
LOCUS       DALAGD010000152         4074 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.157, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000152 DALAGD010000000
VERSION     DALAGD010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.157"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(13..237)
                     /locus_tag="O2701_002626"
     CDS             complement(13..237)
                     /locus_tag="O2701_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294488.1"
                     /translation="MDYAHLNLEHFFARNDDLDVIRDRADFVMINNFTNEMMYRDGQI
                     EGTIDLNQYYYKNRSNAASFIMMDYKKETK"
     gene            436..906
                     /gene="sigS"
                     /locus_tag="O2701_002627"
     CDS             436..906
                     /gene="sigS"
                     /locus_tag="O2701_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigS"
                     /protein_id="HCZ4294489.1"
                     /translation="MKFNDVYNKHHKIIHHLLKKYNISYNYDEYYQLLLIKMWQLSQI
                     YKPSSKQSLSSFLFTRLNFYLIDLFRQQNQLKDVILCENNSPTLTEQPTYFNEHDLRL
                     QDIFKLLNQRERLWLKLYLEGYKQFEIAEIMSLSLSTIKLIKMSVKRKCQHNFN"
     gene            1019..1462
                     /locus_tag="O2701_002628"
     CDS             1019..1462
                     /locus_tag="O2701_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HCZ4294490.1"
                     /translation="MQDNSTKYLLYIQTATSNHLETNCVFLHCDYILKVPINKLVSYY
                     AKLHLSSQSVLIETAKNILNINKLVPIYINPKTILFPLKHKRAPIQIYINAHYIVGMT
                     AIENSTLIHFQEGIQLEVDEPFSLVSKKCHESLALKHFIENTISN"
     gene            complement(1449..1892)
                     /locus_tag="O2701_002629"
     CDS             complement(1449..1892)
                     /locus_tag="O2701_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294491.1"
                     /translation="MNKASFDKKVKKQLWFLNKKEKQALDQRLSSISDDDSVNLNKPV
                     TFANAYLRQNVFRNKETKSYSMFVTLVVMMFAYVALLGLFLFGLITSLSGVQFFVSPK
                     VDLSTTVVILTIIGAILLMFASIYFIKIVTSYFTKKLLEIKFNSK"
     gene            2188..2823
                     /gene="sdpA"
                     /locus_tag="O2701_002630"
     CDS             2188..2823
                     /gene="sdpA"
                     /locus_tag="O2701_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpA"
                     /protein_id="HCZ4294492.1"
                     /translation="MQKFKDFFYDDLSVTRGNYFLTLMAAFFITIILFIGIVVSEVHL
                     LYSMLIVLVGLILLRLFKINLFSFKKLTLSQVIYIIGGALLIYGLDNLYLYFHDVPAN
                     EQQLEQAIRNTPFYISIFTVTIIPAIVEEIVFRGMIIRVIFRKHLFLGLIVSSLVFAS
                     LHESDTWIGYLPYLYSGLIFGLIYIKTKRLEVVIFMHFLNNLLALLFIIWR"
     gene            complement(3263..3976)
                     /locus_tag="O2701_002631"
     CDS             complement(3263..3976)
                     /locus_tag="O2701_002631"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCZ4294493.1"
                     /translation="MAKLNIEVFADGADIEEMKAAYKNKQVDGFTTNPSLMAKAGVTD
                     YKAFAEEAVKEIPDASISFEVFADDLETMEKEAAILKQYGENVFVKIPIVNTKGESTI
                     PLIKKLSADNVRLNVTAVYTIEQVKEITEAVTEGVPTYVSVFAGRIADTGVDPLPLMK
                     EAVKVTHSKDGVKLLWASCRELFNVIQADEIGADIITCPADVVKKVNTNLGRDINELS
                     VDTVKGFAKDIQSSGLSIL"
BASE COUNT         1388 a          666 c          491 g         1529 t
ORIGIN      
        1 tcttttgttc gtttacttag tttctttttt ataatccatc ataataaaac ttgctgcatt
       61 tgatctattt ttataatagt actgatttaa atcaatcgtg ccttcaattt gaccatcacg
      121 atacatcatt tcattagtga agttatttat catcacgaaa tcagcgcgat ctcttataac
      181 atctaaatcg tcgtttcgtg caaaaaaatg ttctaaattt aaatgtgcgt aatccattgt
      241 tacctcccga ttacaaattt tgttctatct actatttagc aaagtaataa tacatcatat
      301 cacattaaat taattactta tgtcttatat ttaacactta atttagcgat tacgcaaatg
      361 aatgattttt ctaaatattt tattctaact ttatccggaa atatttgtta tacgtataac
      421 ttggagaatg actacttgaa atttaatgac gtatacaaca aacaccacaa aatcatacac
      481 catcttttaa aaaaatataa tattagctat aattatgatg agtattatca actactcttg
      541 ataaaaatgt ggcaattgag tcagatatat aaaccctcaa gcaagcaatc tttatcctct
      601 tttttattca ctcgattaaa tttttacctt atcgatttat tcagacaaca aaatcaatta
      661 aaagatgtca ttttatgtga gaataattca ccaacattaa ctgaacaacc aacatacttt
      721 aatgaacatg accttcgttt acaagatatc ttcaagcttt taaatcaaag agaaagacta
      781 tggctcaaac tataccttga aggatacaag caatttgaaa ttgctgaaat catgtcatta
      841 tcgctttcaa cgattaaatt aattaagatg tccgttaagc gtaaatgcca acataatttt
      901 aattagttta ttgtgaaatt tcggtcgatg tgttaatgat gttgaaacac cctaacatct
      961 tcatataaat ttattttaac cattaaatta acaacttatt aaacgaggag tactatattt
     1021 gcaagacaat tctactaaat atctacttta tatccaaact gctacttcaa accatctcga
     1081 aacaaattgt gtctttttac attgcgatta cattcttaaa gttccaatta acaaactcgt
     1141 ttcatattat gcgaaattgc atttatcatc acaaagtgtg ctaattgaga ctgcaaaaaa
     1201 catactaaat attaataaac tggttcctat ttacatcaac ccaaaaacta tactttttcc
     1261 gttaaaacat aaacgtgcac cgatacaaat ttatatcaat gcacattata ttgttggtat
     1321 gactgccata gagaattcaa cattaataca ttttcaagaa ggcattcagc tagaagttga
     1381 tgagccattt tctctagttt caaaaaaatg tcatgaaagt ttagctttga agcacttcat
     1441 tgaaaatact atttcgaatt aaatttaatt tctaaaagtt tcttagtaaa gtatgatgtc
     1501 acaattttta taaaatatat gctagcaaac attaataaaa tcgctccaat aattgttaaa
     1561 ataacaacag ttgtagataa atctacctta ggactaacga aaaattgtac gccagataga
     1621 cttgtaatta gcccaaataa aaacaaacct aataaagcaa cataagcaaa catcatcact
     1681 actaatgtaa caaacatact ataacttttt gtttctttat ttcgaaatac attttgtctt
     1741 aagtaagcat tagcaaaggt aacaggctta tttaagttaa cgctgtcatc atcagatata
     1801 gaagacaatc gttgatctaa tgcttgtttc tccttcttgt tcaaaaacca taattgcttt
     1861 ttaacttttt tatcaaatga tgctttattc ataattaagg atttctctcc tttataatat
     1921 tgacgtaatt ttaaacatta gcgtcaattg aaagtgtatt ttatatattt tacagaaata
     1981 tatgtatcaa atctacaaaa atccatatta caatttcatt tatggtgatt tgacgctaaa
     2041 atacagtctg ctaatttttc aaactaatgc tttttaggta ggtacaatga taaattttaa
     2101 tcgtaaatat gtttcatata ttatgaatct agccttaatc atattgcatt caaaataatt
     2161 ttaaaaaatg aaaagaaagg atttagcatg caaaaattca aagacttttt ttacgatgat
     2221 ttatcggtta cacgaggaaa ttatttttta actttaatgg cagcattttt tattactatc
     2281 attttattta tcggcatagt tgtcagtgaa gtacatttac tttatagcat gctaattgta
     2341 ttagtaggtt taattctatt gaggctattc aaaatcaatt tattctcttt taaaaaatta
     2401 acattgtctc aagttattta tattataggc ggtgcactat taatttatgg gttagataat
     2461 ctttatttat attttcatga cgtaccggca aatgaacaac aattagagca agcaatacga
     2521 aatacaccat tctatatttc tattttcact gttaccatca tccccgctat tgtggaagaa
     2581 attgtttttc gcggtatgat aataagggtt atcttcagaa aacacttgtt tttagggtta
     2641 attgtgtcta gtttagtttt tgcatcatta cacgaatctg acacttggat tggttattta
     2701 ccttacttat attctggttt gatttttggc ctaatttata taaaaacaaa acgattagaa
     2761 gttgtaatat tcatgcactt cttaaataac ttgttagctc tgctctttat aatatggaga
     2821 taattttgag ataacctttc atattatttt caaattatct atctgatatt tattttattg
     2881 aacttgagtc accacttaaa ctcaaaaata cgtgtcatca agtgcaaaat aatttagaac
     2941 taatacaaac ataaaaactt ttactttacg cttaaaatca aaagtcacat acctagatat
     3001 aagtacaact tgtatatttt gtactctaat aggtatgtgg cttaaataat atttaattct
     3061 tgagttgcat atagtaactg tttttattca ttttctaaaa tatgcaagac taatttcctt
     3121 ttctactcct gttgaatcaa tttagacttt tttataactc gagcttttac ttagctgaat
     3181 tttgacttct tcttcattaa taactatgat ggaaaaaccc ccataagcac aatgcctatg
     3241 agggtttaaa tatatttata tattatagaa tagaaagacc tgaagattga atatctttcg
     3301 caaagccttt aactgtatct actgataatt cgttaatatc gcgacctaag tttgtattca
     3361 cttttttcac aacatctgct gggcatgtaa taatatctgc accaatttca tcagcttgaa
     3421 tcacattgaa taattcgcgg caacttgccc ataataattt aacgccgtct ttactatgcg
     3481 taacttttac agcctctttc attaatggta atggatctac gcctgtatct gcaatacgtc
     3541 ctgcaaatac tgaaacatat gttggcacac cttcagttac tgcttcagtt atttctttaa
     3601 cttgttcaat tgtgtaaaca gccgtaacgt ttaatctcac attgtcagct gaaagttttt
     3661 taattaaagg aatcgttgat tcaccttttg tatttacaat aggaatttta acaaatacat
     3721 tttcgccata ttgttttaaa attgctgctt ctttttccat agtttctaaa tcgtctgcaa
     3781 atacttcaaa tgaaattgaa gcatctggaa tttctttcac agcttcttca gcaaaagctt
     3841 tgtaatctgt tacgcccgct ttagccatta aactaggatt tgttgtaaaa ccatccactt
     3901 gtttgttttt ataagctgct ttcatttctt caatatctgc accgtccgca aatacttcta
     3961 tatttagttt agccatataa tatagcctcc ttgattctta ttaaaatttt aacaacatct
     4021 gcatgtcttt ttcttacaac catttgtaaa aaatgatttt tatttctttg ttat
//
LOCUS       DALAGD010000153         4012 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.158, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000153 DALAGD010000000
VERSION     DALAGD010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.158"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            763..2826
                     /locus_tag="O2701_002632"
     CDS             763..2826
                     /locus_tag="O2701_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HCZ4294494.1"
                     /translation="MNNNGEEHNHQNHMNHSNQMHHDNHASHDHHSGHAHHHGNFKVK
                     FFVSLIFAIPIILLSPLMGVNLPFQFTFPGSEWVVLILSTILFFYGGKPFLSGGKDEI
                     ATKKPGMMTLVALGISVAYIYSLYAFYMNNFSSATGHTMDFFWELATLILIMLLGHWI
                     EMNAVGNAGDALKKMAELLPNSAIKVMDNGQREEVKISDIMTDDIVEVKAGESIPTDG
                     IIVQGQTSIDESLVTGESKKVQKNQNDNVIGGSINGSGTIQVKVTAVGEDGYLSQVMG
                     LVNQAQNDKSSAELLSDKVAGYLFYFAVSVGVISFIVWMLIQNDVDFALERLVTVLVI
                     ACPHALGLAIPLVTARSTSIGAHNGLIIKNRESVEIAQHIDYVMMDKTGTLTEGNFSV
                     NHYESFKNDLSNDTILSLFASLESQSNHPLAISIVDFAKSKNVSFTNPQDVNNIPGVG
                     LEGLIDNKTYKITNVSYLDKHKLNYDDDLFTKLAQQGNSISYLIEDQQVIGMIAQGDQ
                     IKESSKQMVADLLSRNITPVMLTGDNNEVAHAVAKELGISDVHAQLMPEDKESIIKDY
                     QSDGNKVMMVGDGINDAPSLIRADIGIAIGAGTDVAVDSGDIILVKSNPSDIIHFLTL
                     SNNTMRKMVQNLWWGAGYNIVAVPLAAGILAFIGLILSPAIGAILMSLSTIIVAINAF
                     TLKLK"
     gene            2844..3389
                     /locus_tag="O2701_002633"
     CDS             2844..3389
                     /locus_tag="O2701_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1541 domain-containing protein"
                     /protein_id="HCZ4294495.1"
                     /translation="MIKKLFFMILGSLLILSACSNNDEKDKDTNDQKSESHMKHNDES
                     KVPEDMKSTNEGEFKVGDKVTITAGHMPGMKGAEATVKGAYKTYAYVVSYKPTNGNEK
                     VNNHKWVVNEEIKDAPKDGFSKGDTVKLEASHMSGMKGATANIDNVKKTTVYVVDYKS
                     KDNGKIIKNHKWMTGNELKAR"
     gene            complement(3817..3951)
                     /locus_tag="O2701_002634"
     CDS             complement(3817..3951)
                     /locus_tag="O2701_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294496.1"
                     /translation="MKLHWYIYYFLSISVILFMIIYHYLATSMTVDYEITVSIAKLLL
                     "
     gene            3915..4007
                     /locus_tag="O2701_002635"
     CDS             3915..4007
                     /locus_tag="O2701_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294497.1"
                     /translation="MTKNSKYTNEVSKEQFQEIENVNNKVKEFY"
BASE COUNT         1477 a          536 c          713 g         1286 t
ORIGIN      
        1 attgcctcgt ggttctgagc ttgaacttat ctctaaatca ttttgagcta cttatctatc
       61 aattcatata ttctataaca atatttgtga catcacgtgc tatttcatga agtgatttta
      121 cgatatcacc ttctttagaa aaaatatatt tcagtaggca ccgacgtata cagaatcatt
      181 tgagtattaa aaataaaaac tagaaaaaga aaccgcatca ttaactgata cgcagaatca
      241 tattataaat aaaactaaaa atgaggttgt atataactca ctctgaaatt gattgaatat
      301 atagtatctt taataaaatg cagctattgt ggcgtagaat ttgagaatca aaaaatgatt
      361 aatatagttt gaagagactg agcataaata ctagaaaaat ggccagtaaa tgagtttact
      421 ataaactcat ttactggctt ctctaataat tatcaagaca atttgcgttt ctaggcatac
      481 tttgaaatgc gctattttct ttaagaatat taatatatga cttgtgttgg taatgataac
      541 cttggttaga gtgtattgtc atgtgatagt taagattaga tcttttaatt aaaacattgt
      601 tttaaatatc gatgacaagg tctaatgtag gacgtgtaga tataaggttc ggaattatat
      661 aaatccataa aaggagataa atcgaaaacc tttataccct gggtgggtat atgttatagt
      721 ataagtagct ttactataac attttcatta ggaggggtta atttgaataa taatggtgaa
      781 gagcataatc atcaaaatca catgaatcat tccaatcaaa tgcatcatga taaccatgcc
      841 tcacatgatc atcatagtgg ccatgcacat catcatggaa attttaaagt taagtttttt
      901 gtttcattaa tttttgcaat acctatcatt cttttatcgc cactgatggg tgttaactta
      961 ccttttcaat tcacatttcc aggttctgaa tgggtagtgt taatattaag tacaatttta
     1021 ttcttttatg gtggtaaacc gttcttgtct ggtggtaaag atgaaattgc tacaaaaaaa
     1081 ccaggcatga tgaccttagt tgccctaggt atttcagtag cttatattta tagcttgtat
     1141 gctttttata tgaataactt tagtagtgca actggtcata caatggactt tttttgggaa
     1201 ttagcaacct taattttaat tatgctatta ggacattgga tagaaatgaa tgctgtcgga
     1261 aatgctggag atgctttaaa gaaaatggca gaactgttac ctaatagtgc tattaaagtt
     1321 atggataatg gccaacgcga agaagttaaa atatcagaca tcatgactga tgatatcgtc
     1381 gaagtaaaag ccggagaaag cattccaaca gatggtatta tcgttcaagg acaaacatct
     1441 atagatgaat ccctagtcac tggagaatct aaaaaagtac aaaaaaatca aaatgacaac
     1501 gtcatcgggg gttctattaa tgggtctgga acaatacaag tcaaggttac agctgtggga
     1561 gaagatggat atctttctca agttatggga cttgttaatc aagcacaaaa tgataaatct
     1621 agtgctgaat tgttatctga taaagtagcg ggttatttat tctactttgc tgtaagtgtt
     1681 ggcgtgattt cttttattgt ctggatgctc attcaaaatg atgttgattt tgcattagaa
     1741 cgtcttgtaa ctgtgttagt cattgcttgt ccacatgctt taggcttggc aataccttta
     1801 gtcactgcac gttctacttc aattggtgca cataatggtt taattattaa aaatagagag
     1861 tctgtagaaa tagctcaaca tatcgattat gtaatgatgg acaaaactgg tactttaact
     1921 gagggtaact tttctgtgaa tcattatgag agctttaaaa atgatttgag taatgataca
     1981 atattaagcc ttttcgcctc attagaaagt caatctaatc acccattagc tataagtatt
     2041 gttgattttg cgaaaagtaa aaatgtttca tttactaacc cacaagacgt taataatatt
     2101 ccaggtgtcg gattagaagg tctaattgat aataaaacat ataaaataac aaatgtctct
     2161 tatcttgata aacataaact taattatgac gatgacttgt ttactaaatt agctcaacaa
     2221 ggtaattcaa tcagttattt aattgaggat caacaagtca ttggcatgat tgctcaagga
     2281 gatcaaatta aagaaagctc aaaacaaatg gtagctgatt tactatcaag aaatattaca
     2341 ccagtcatgc ttacaggtga caataatgaa gtggcacacg ctgtcgcaaa agaattaggt
     2401 attagtgatg tccacgcaca actcatgcca gaagataagg aaagcattat aaaagattat
     2461 caaagtgacg gtaataaagt catgatggtc ggagacggta tcaacgatgc gccgagtctt
     2521 ataagagcgg atattggtat agcaattggt gcaggtacag atgttgcagt ggattcaggt
     2581 gatatcatac ttgttaaaag taatccatca gatatcattc atttcttgac cctttcaaat
     2641 aatactatga gaaaaatggt gcaaaactta tggtggggtg caggttataa tattgttgct
     2701 gtacctttag cagctggtat tttagcattt attggcttga ttttatcacc tgcaataggt
     2761 gctattttaa tgtctttaag tacaattatc gttgcaatta atgcctttac attaaaatta
     2821 aaataaaaga taggagtttt attatgatta aaaaattatt ttttatgata ttaggatcat
     2881 tactaatatt atcagcttgc tccaataatg atgaaaaaga taaagacact aatgaccaaa
     2941 aaagtgagag ccatatgaag cataatgatg aaagtaaagt tccagaagat atgaaatcga
     3001 ctaatgaggg tgaatttaaa gtgggagata aagtaacgat tacagcaggg catatgccag
     3061 gtatgaaagg tgcagaagct actgtaaaag gtgcgtataa aacatatgcc tatgttgtaa
     3121 gttataaacc cacaaatgga aatgaaaaag taaacaatca taaatgggtc gtaaacgaag
     3181 agattaaaga tgcacctaaa gatggattta gtaagggcga tactgttaaa ttagaagcaa
     3241 gtcatatgtc tggtatgaaa ggtgctacag ccaatataga taacgtgaaa aagactactg
     3301 tttacgtagt tgattacaaa tccaaagata atggtaaaat cattaaaaat cataaatgga
     3361 tgacaggaaa tgagctgaaa gcacgataaa aatctagttc taaattgaga aataaataga
     3421 tataaaaata tcctccttaa tcaataattt aaataactta ttattgttaa ggaggatatt
     3481 ttttagtgtg taaattaaaa agaattttag aagaataaca tttatcaaaa aactgttcat
     3541 taccttatta attgaaatta tataattaaa aaccgcatca ttaaccgata cgcagaggcg
     3601 tatcataagt aaaactaaaa aattctgtat gaggagataa taatttggag ggtgttaaat
     3661 ggtggacatt aaatccacgt tcattcaata tataagatat atcacgataa ttgcgcatat
     3721 aacttaagta gtagctaaca gttgaaatta ggccctatca aattggttta tatctaaaat
     3781 gattaatata gaatgcttct ttttgtcctt attaaattat aaaagtaact ttgcaataga
     3841 aacagttatt tcataatcaa cagtcattga cgtagctaag taatgataaa taatcataaa
     3901 taaaattaca gatattgaca aaaaatagta aatataccaa tgaagtttca aaagaacaat
     3961 tccaagaaat tgagaatgta aataataagg tcaaagaatt ttattaagat tt
//
LOCUS       DALAGD010000154         3795 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.159, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000154 DALAGD010000000
VERSION     DALAGD010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.159"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            113..487
                     /locus_tag="O2701_002636"
     CDS             113..487
                     /locus_tag="O2701_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HCZ4294498.1"
                     /translation="MAITKINDCFELLSMVTYADKLKSLIKKEFSISFEEFAVLTYIS
                     ENKEKEYYLKDIINHLNYKQPQVVKAVKILSQEDYFDKKRNEHDERTVLILVNAQQRK
                     KIESLLSRVNKRITEANNEIEL"
     gene            complement(738..1526)
                     /locus_tag="O2701_002637"
     CDS             complement(738..1526)
                     /locus_tag="O2701_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCZ4294499.1"
                     /translation="MNKVTINPQIQLTYQIEGKGDPIILLHGLDGNLAGFEDLQHQLA
                     SSYKVLTYDLRGHGKSSKSESYDLNDHVEDLKILMEKLNIHEAHILGHDLGGVVAKLF
                     TDKYAYRVKSLTTIASKKDDLIHSFTQLLIQYQDDIAGFNKSEAYILLFSKLFRNQEK
                     TMKWYQKQRIYSIKSEDDSAVAIRSLILHKDEPMYLKKRTCVPTLLINGEHDPLIKDK
                     NHFKLEAHFLNVTKKIFEHSGHAPHIEEPEAFMNYYLKFLKSVS"
     gene            complement(1695..2411)
                     /locus_tag="O2701_002638"
     CDS             complement(1695..2411)
                     /locus_tag="O2701_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294500.1"
                     /translation="MRINDKILLENIEDYFNHKGLSPHLIDDIKEKVITDIKNSEKKD
                     QDYIEYKRKSPAQIILMIQRNLFALQMNPVIFFIINFILISYLYDKQYVQFQAITGMS
                     LFYCLVIFPMTIVVYLRVSQKNYLRSNKIEMIMGTIIAIISLLLIILQAFNITWGVIP
                     ITNFGHQFFFFIGIILVIAGIFYKRLEFSGIGLLFCQKTVDAMIHNPQSAQIFSLIIW
                     ILLVVLVIYFTIRLSSRTRL"
     gene            complement(2573..3079)
                     /locus_tag="O2701_002639"
     CDS             complement(2573..3079)
                     /locus_tag="O2701_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294501.1"
                     /translation="MKKLLTASIIACSVVMGVGLVNTSAEAASGNSIDTVKQLIKGDQ
                     SLENVKIGESIKDVLTKYKNPMYSYNEDGTEHYYEFHTKKGMLLVTTDGKKNNGKVTH
                     ISMMYNDANGPTYQAVKNYVGKAVTHTEYSKVAGNFGYIEKGKTTYQFASAPKDKNIK
                     LYRIDLEK"
     gene            complement(3215..>3795)
                     /locus_tag="O2701_002640"
     CDS             complement(3215..>3795)
                     /locus_tag="O2701_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCZ4294502.1"
                     /translation="IVELLKYLYIEEVAFVCHEMGGIIGADISVRYPEFTSSLTLVNP
                     TSIEGELPEERLFRKYAHIIRNWDPEKQDKFLNKRKYYRPRKMNRFLKHVVDTNEIST
                     KEEIQAVKEVFKNADISQTYRNVVVPTKIIAGEFGERTTRLEAKEVADLIQNADFEVY
                     QESSAFPFVEEQERFVEDTAAFINKHHDEKHV"
BASE COUNT         1310 a          616 c          479 g         1390 t
ORIGIN      
        1 ttatttaatg caaaattatg actaacatat ctataataaa taaagattag atatcaatat
       61 attatcgggc aaatgtatcg agcaagatgc atcaaatagg gaggttttaa acatggcaat
      121 tacaaaaatc aatgattgct ttgagttgtt atcaatggtc acttatgctg acaaattaaa
      181 aagtttaatt aaaaaggaat tttcaattag ctttgaagaa ttcgctgtat tgacatacat
      241 cagcgaaaac aaagagaaag aatactatct taaagatatt attaatcatt taaactacaa
      301 acaaccacaa gttgttaaag cagttaaaat tttatctcaa gaagattact tcgataaaaa
      361 acgtaatgag catgatgaaa gaactgtatt aattcttgtt aatgcacaac aacgtaaaaa
      421 aatcgaatca ttattgagtc gagtaaataa acgaatcact gaagcaaaca acgaaattga
      481 actataattt tgtttagcgc aatttggtga agtttgatag atgatacatt ctattaaact
      541 tccttttttt atgctctttt tacctaattg ttaagaggtt ttgcactaat ggcacttaat
      601 ataaggaaga tagccgcata acgagcagta tatatcaaat acatttcaaa aaaagctgat
      661 ttctatcaaa taatgaatag taatcagctt tttcacattg cctaaggact ttatgcccta
      721 ggtttatata tcacatatta tgatacgctt tttaaaaatt ttaaataata attcataaat
      781 gcttctggtt cttcaatatg cggtgcatgt cctgaatgtt cgaagatttt tttcgtaaca
      841 tttaaaaaat gcgcttccaa tttaaaatga ttcttatctt taatcaaagg atcatgttcc
      901 ccattaatta acaaagtagg tacacatgta cgttttttta aatacatagg ttcatcttta
      961 tgcaaaatta atgaacgaat tgccaccgca ctatcatcct cagacttaat gctatatatt
     1021 ctttgttttt gataccattt catcgtcttc tcttgatttc taaacaattt agaaaataaa
     1081 agaatatacg cttcagactt attaaaaccc gctatatcat cttgatattg tattaacaat
     1141 tgagtaaagc tgtgtattaa gtcatctttc ttcgatgcaa tggtagttaa tgattttaca
     1201 cgataagcat atttatctgt aaataactta gcaactaccc cacctaaatc atgtcctaga
     1261 atatgtgcct catgaatatt taacttctcc attagaattt ttaaatcctc aacgtgatcg
     1321 tttaaatcgt atgattcact tttagaagac ttgccatgac ctcttaaatc gtaagtaagt
     1381 actttatatg atgatgctag ttgatgttgc aaatcttcaa atccagctaa attaccatcc
     1441 aatccatgaa gtaatattat aggatcccct ttaccttcaa tttgataagt taattggatt
     1501 tgaggattaa ttgtgacttt attcattgtg acacctccct caataaacta tatacccaac
     1561 cttcattcta tatacgcttc cattttataa acaatttttt taaattattt ataaattacc
     1621 aaaagttatt ccaacaataa aaaaactatt catctcaatt aatacaatta atcaagtgaa
     1681 tagttttatc atatttataa tcttgtacgt gaagataatc taattgtgaa atatataact
     1741 agaactacta ataatatcca tataattaat gaaaaaatct gggctgattg tggattatga
     1801 atcattgcat cgacggtttt ttgacaaaat aataacccga ttcccgaaaa ctcaagtcgc
     1861 ttataaaata tgccggcaat tactaaaata ataccaatga aaaagaaaaa ttgatgtcca
     1921 aaatttgtaa ttggtataac gccccaagta atattaaatg cttgtaatat aattaataac
     1981 aaggatatga tagcgataat tgtacccata atcatttcta ttttattact acgcaagtaa
     2041 ttcttttgag acacccttag gtatacaaca atagtcattg gaaaaatcac taaacaataa
     2101 aatagactca ttccagtaat agcttgaaac tgaacatact gtttatcata taaataggat
     2161 atgagaatga agtttataat aaagaaaata actggattca tttgtaaagc aaacaaattc
     2221 ctttgaatca ttaatatgat ttgtgctgga gattttcttt tatattcaat ataatcttga
     2281 tctttctttt cagaattttt tatatcagta attactttct ctttaatatc atcaatcaaa
     2341 tgcggtgata aacctttatg attaaagtaa tcttctatat tttctagtaa aattttatcg
     2401 ttaatacgca ttggtagtac tcctttaaaa tgaaacatct ttatttattg tattggttta
     2461 atttcccctt cgcaaatcat aaaaattaat attcactgta ttatgccatg taataaaata
     2521 accgacaagt attgcgattt aacttgtcgg ttaagcacga ttaatttaat tattattttt
     2581 ccaaatcaat acgatataat tttatgtttt tatcttttgg tgctgaggca aattgataag
     2641 tcgttttgcc tttttcaata tatccaaaat taccagcaac tttgctatat tccgtatgtg
     2701 ttactgcttt gccaacataa tttttaacag cttgatatgt tggaccatta gcatcattat
     2761 acatcattga aatatgagtt actttaccat tgtttttctt accatcagta gttactaata
     2821 acataccttt tttagtatgg aattcgtaat aatgttcagt tccatcttca ttgtaagaat
     2881 acatcggatt tttgtactta gttaaaacat ctttaataga ttcgccaatt ttcacatttt
     2941 ctaatgactg atcaccctta attaattgtt taacagtatc aatagagttg ccacttgctg
     3001 cttcggcact agtgttcact aagcctactc ccattacaac agaacatgca attatacttg
     3061 ccgttagtag ctttttcata agcactctct ccttttattt atatcgtctt gtgctcacaa
     3121 ccattataca acaatcattt ctaaaacaac agtcatatta caattatatt acaaataata
     3181 attactactt ttatatttca cttatcacta aaaattaaac atgcttttca tcgtgatgtt
     3241 tgttgataaa tgcagctgta tcttcgacga atctttcttg ctcttcaaca aatgggaatg
     3301 cacttgattc ttgatatact tcaaagtccg cattttggat taaatcagct acttctttag
     3361 cttctaatct tgttgttctt tcaccgaatt ctcctgcaat aatttttgtc ggtactacga
     3421 catttctata agtttgagaa atatcagcgt ttttgaatac ctctttaact gcttgaattt
     3481 cttctttagt tgatatttca tttgtatcta cgacatgttt gaggaatcga ttcatttttc
     3541 tcggacgata atacttacgc ttatttaaaa atttatcttg tttttcagga tcccagtttc
     3601 gaataatatg ggcatatttt ctaaataaac gttcttccgg taattcacct tcaatagatg
     3661 ttggatttac caacgtaagt gatgatgtaa attcaggata acgtactgaa atatccgcac
     3721 caatgattcc acccatttca tggcatacaa atgcaacttc ttcaatatat aaatatttaa
     3781 gtaattcaac aatgt
//
LOCUS       DALAGD010000155         3786 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.160, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000155 DALAGD010000000
VERSION     DALAGD010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.160"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(221..553)
                     /locus_tag="O2701_002641"
     CDS             complement(221..553)
                     /locus_tag="O2701_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HCZ4294503.1"
                     /translation="MGKKMGLGLSIALVVIGIAVVCLMIFSSQKTTYFGYMNSNTNAE
                     KVVSEKDGLVKHNIKVEPSNDFKPKKGDFVKLVSKDDGKTFYKQEIVKHDDVPHGLMM
                     KIHDMHMN"
     gene            complement(637..1782)
                     /locus_tag="O2701_002642"
     CDS             complement(637..1782)
                     /locus_tag="O2701_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HCZ4294504.1"
                     /translation="MFQLLAVCPMGLEAVVAREIQELGYETNVENGRIFFEGDASAIV
                     KANLWLRTADRIKIVVGRFNATTFDELFEQTKALPWESIIDKEGNFPVQGRSVKSTLH
                     SVPDCQAITKKAIVERLRRAYNEKGWLNESGAKYPVEVAILKDNVLLTIDTSGSGLNR
                     RGYRLAQGEAPIKETLAASLIRLANWKGDTPLIDPFCGSGTIAIEACLIAQNIAPGFN
                     REFVSEQWNIMPANIYDDYRDEADKMADYDKEIEVYASDIDPEMVEIAKRNAEEVGLS
                     DIIKFSVKDVNTLTIDTEEPVALIGNPPYGERIGDREEVEEMYRYIGKLMKQHPFLST
                     YILTSNKEFEYLVDRKATKRRKLFNGYIECTYYQYWGKKTERKTIEN"
     gene            complement(1991..2370)
                     /gene="rnpB"
                     /locus_tag="O2701_002643"
     ncRNA           complement(1991..2370)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="O2701_002643"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(2433..2777)
                     /gene="gpsB"
                     /locus_tag="O2701_002644"
     CDS             complement(2433..2777)
                     /gene="gpsB"
                     /locus_tag="O2701_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HCZ4294505.1"
                     /translation="MSDVSLKLSAKDIYEKDFEKTMARGYRREEVDAFLDDIIADYQK
                     MADMNNEVVKLSEENHKLKKELEELRLRVATSRPQDNKSFSSNNTTTNTSSNNVDILK
                     RISNLEKAVFGK"
     gene            complement(2791..3354)
                     /locus_tag="O2701_002645"
     CDS             complement(2791..3354)
                     /locus_tag="O2701_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HCZ4294506.1"
                     /translation="MVKTVYVTGYKSFELNIFKDDAPEVHYLKQFIKHKIEQLLDEGL
                     EWVLIQGQMGIELWTAEVVIELQRTYDSLKFAVITPFQGHTEKWNEHNQSKYANIIKH
                     ADYVDSIFHTSYQGPFQFKQADQFMLEHSDQTLLIYDEEQEASPKFFKQMLVDFMDKT
                     NYTCDIVTFDELTAFINDLQWSEDQSF"
     gene            complement(3347..3697)
                     /locus_tag="O2701_002646"
     CDS             complement(3347..3697)
                     /locus_tag="O2701_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YppE family protein"
                     /protein_id="HCZ4294507.1"
                     /translation="MNDVVESLIYEVNNMQQNFENVKSQQQDHDFYQTVKPYTEHIDS
                     ILNEIKLHREFIIEVPYMNSRKFELLIANIEQLSVECHFKRTSRKLFIEKLKSVQYDL
                     QNILDGVTKEGTDG"
BASE COUNT         1147 a          722 c          498 g         1419 t
ORIGIN      
        1 cgtgctaaac aataatgcac ttaaagtttt gaactgacga aatttacaaa atgattctcg
       61 tctctctaat tacttaaaac gggttctata ataaatcgta ctgatggaaa gtttttactt
      121 tttatctgtc cgattttttg aatttgaaga taaaaaaaca tctaaaacgc ataagaagat
      181 aactctactt cttcaaaatt acgttataga tgctttttta ttaattcata tgcatgtcgt
      241 gaattttcat cattaaaccg tgtgggacgt catcatgttt aacaatctct tgtttataaa
      301 atgtcttccc atcatcttta gaaactaatt ttacaaagtc tccttttttc ggcttgaaat
      361 cattagatgg ttctactttg atattatgtt tgactaatcc atctttttca ctgacaactt
      421 tttctgcatt tgtattacta ttcatataac caaaataagt cgttttttga ctagaaaaaa
      481 tcattaaaca tacaacggca ataccaataa caaccaatgc aatagataaa cctagaccca
      541 tttttttacc catttataga ctccttcatt tttcaaaaat acctattatc atgagaactt
      601 aattattact aattttagtc acgcattaga aacactttag ttttcaatag ttttacgttc
      661 tgtcttttta ccccagtatt gataataagt acattctata tatccattaa ataacttacg
      721 tcgctttgtt gctttacgat ctactaaata ttcaaattct ttattacttg ttaaaatgta
      781 tgtagataaa aatggatgtt gtttcattag tttaccaata taacggtaca tttcttcaac
      841 ttcttcacga tcaccaatac gttcaccata tggaggattt ccaattaacg ccaccggttc
      901 ttctgtatca attgttaatg tattgacatc ttttacacta aatttaataa tatcagacaa
      961 tccaacttct tcagcgttac gcttagcaat ctctaccatt tctggatcga tatcagaagc
     1021 atatacttcg atttctttat cataatcagc catcttatcc gcttcatcac ggtaatcatc
     1081 ataaatattt gctggcatga tgttccattg ctctgatacg aactcgcgat taaaaccagg
     1141 tgcgatattt tgagcaatta aacaagcttc tatagctatt gttcccgaac cgcaaaatgg
     1201 atcaattaaa ggtgtatcac ctttccagtt tgcaagacgg attaaacttg ctgccaacgt
     1261 ttctttaatt ggtgcttcac cttgtgctaa tctataacca cgtctgttca aaccagaacc
     1321 tgatgtgtcg atagtcaata atacattatc ttttaaaatg gcaacttcaa cagggtattt
     1381 ggcacctgat tcatttaacc aacctttttc gttatatgcg cgacgtaatc gttcaacaat
     1441 agctttctta gttatcgcct gacaatctgg cacactatgt agtgttgatt taacgcttct
     1501 accttgaact gggaagttac cctctttatc aattatagat tcccaaggga gcgctttggt
     1561 ttgttcgaat aattcgtcaa acgttgttgc gttaaaacgt ccaacaacaa ttttgattcg
     1621 gtctgctgtg cgcaaccata aatttgcctt tacaattgca cttgcgtctc cttcaaaaaa
     1681 tatacgacca ttttcaacat ttgtttcata gcctaattct tgaatttccc tagcaacaac
     1741 agcttctaat cccatcggac aaactgcaag taattgaaac atatatgatt ctccttttat
     1801 acaggtattt tattcttagc ttgtgttttt tatacatttc caacaaattt aatcgctgat
     1861 acattaacgc atccgcttac tattttaaaa caaggcagtg tcattatatc aagacaaggc
     1921 gttaatttta agtgttcttt tcatgaaaaa agctctccat catctaggag agctaaacta
     1981 gtagtgatat ttctataagc catgttctgt tccatcgtac tcatcacgtg cactagtcac
     2041 actggtactc aggtgataac catctgtcta caccacttca tttcgcgaag tgtgtctcgt
     2101 ttatacgttg aattccgtta aacaagtgct cctaccaaat ttggattgct cactcgaggg
     2161 gtttaccgcg ttccaccttt tatatttcta taaaagctac gtcactgtgg cactttcaaa
     2221 ttactctatc catatcgaaa gacttaggat atttcattgc cgtcaaatta atgccttgat
     2281 ttattgtttc atcaagcacg aacactacaa tcatctcaga ctgtgtgagc atggactttc
     2341 ctctatataa tatagcgatt acccaaaata tcacttttaa aattataaca tagtcattat
     2401 tagtaagaca gttaaacttt tgtatttagt aattatttac caaatacagc tttttctaag
     2461 tttgaaatac gttttaaaat atctacatta tttgaagatg tatttgttgt tgtattattc
     2521 gaagaaaaac ttttattgtc ctgaggtctt gatgttgcta cacgtagtct taattcttct
     2581 aattcttttt taagtttatg attctcttct gataatttta caacttcatt attcatatcg
     2641 gccatttttt gataatcagc aataatgtca tctaaaaatg catctacttc ttctcttcta
     2701 tagccacgag ccatcgtttt ttcaaaatct ttttcataaa tatcttttgc tgataatttc
     2761 aatgaaacat ctgacatttt ttccacctca ttagaaactt tgatcttcag accactgtaa
     2821 gtcattgatg aatgctgtta attcatcgaa cgtcacaata tcacaagtat agtttgtttt
     2881 atccataaaa tcaactaaca tctgcttgaa gaacttagga ctcgcctctt gttcctcatc
     2941 ataaatgagc aatgtttgat ccgaatgttc aagcataaat tgatctgctt gtttaaattg
     3001 aaaaggacct tgatacgacg tatgaaaaat actatcaaca taatctgcat gcttaattat
     3061 gttggcatac ttactttgat tatgttcatt ccacttttct gtatgtcctt ggaacggtgt
     3121 aattacagca aactttaacg aatcatatgt tcgttgtaat tcaataacaa cttctgcagt
     3181 ccataattct atacccattt gcccttgtat taacacccat tctaatcctt catccaacag
     3241 ttgttcaatt ttatgtttta taaattgttt taaataatgt acttcaggtg cgtcatcttt
     3301 aaaaatgttt aattcgaatg atttgtaacc tgttacataa actgttttaa ccatcagtac
     3361 cctcttttgt tacgccatct aatatatttt gtaaatcata ttgaacactt ttaagctttt
     3421 ctataaataa ctttcgactt gttcgcttaa aatgacattc gacagaaagt tgttcaatgt
     3481 tagcaatcag tagctcaaat ttccttgaat tcatataagg tacttctata ataaattcac
     3541 gatgtaattt gatctcattg agtatgctat caatatgttc agtatatggc tttacagttt
     3601 ggtaaaaatc atgatcttgt tgttgtgatt tcacattttc aaaattttgt tgcatgttgt
     3661 taacttcata aattagtgat tcgactacat cattcatgag gacgcctcct acatttttta
     3721 atttatcaca atatactgta ttcgtcatgt tttaacactc tatataattt gatttaacta
     3781 tttttt
//
LOCUS       DALAGD010000156         3777 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.161, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000156 DALAGD010000000
VERSION     DALAGD010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.161"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            64..1554
                     /locus_tag="O2701_002647"
     CDS             64..1554
                     /locus_tag="O2701_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HCZ4294508.1"
                     /translation="MHRESKYIEYKKSRKGLSNDIWSTYSAFANTEGGTIYLGIEEKK
                     IEDKKVFVSVGVEDPEKMIEDFWNALYGRSKVSQNILSNKDVKIVNIENKACIEIHVP
                     EAPYSKKPIYVDNKKDLVYKRVDDADRIATEEEYKFMIVNSQDDIDTELLDNYDMSDL
                     NHESIENYRKLLLKNTNDERYANMSQLDLMIDLGAYRKDRSSKDKQYKMTTACLLFFG
                     KYNAISDRFPGFQLDYFKKTNYLDTDWKDRISSGDLGNEDLNVYSFFEKVLIKLTDNI
                     EESFSLNDGLTRQNYARDLKVAIREALVNTLMHAYYDTKQSIKIVNCEDFIEFYNPGN
                     MRINKEDFIHGGHSKDRNSILSTLFRRVGYSEKAGSGGPRIFDVVNRHKLKTPEIELT
                     DMDTNVVLWKQDLMKEFEKYPELDKKVIKYIIDYGSISKGEALKMENMTEYQFRNILK
                     KLKDDNLIKKEGEGPATKYVLIESKEADILRTKKVIKSLESFFRNK"
     gene            complement(1927..3245)
                     /gene="cstB"
                     /locus_tag="O2701_002648"
                     /pseudo
     CDS             complement(1927..3245)
                     /gene="cstB"
                     /locus_tag="O2701_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002488497.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide dioxygenase-sulfurtransferase CstB"
     gene            3301..3438
                     /locus_tag="O2701_002649"
     CDS             3301..3438
                     /locus_tag="O2701_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001791883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294509.1"
                     /translation="MELKLLNKLDDDQLDLEFLQDNGLKGNVQGPMTLKEPLKSYIQK
                     S"
     gene            3435..>3777
                     /locus_tag="O2701_002650"
     CDS             3435..>3777
                     /locus_tag="O2701_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCZ4294510.1"
                     /translation="MKTNILNLDNELENTEYVEGKPYVQYGIKYERNQALRNEAIKIH
                     GTTCKVCGFDFKAKYGDLGEGFIEIHHLKPMFSIKREIKVNPQKDLVPLCSNCHKMIH
                     RNTKKPLTIKEL"
BASE COUNT         1429 a          460 c          664 g         1224 t
ORIGIN      
        1 tatggtagac ggtttgagta aattaatgga gtattttaat atttatggtt tggaggaatt
       61 gcgttgcata gagaaagtaa atatatagag tataagaaat cacgaaaagg attatctaat
      121 gatatttggt ctacgtatag tgcttttgca aatactgaag gtggtactat atatttagga
      181 attgaagaaa aaaagatcga ggacaaaaaa gtctttgttt cagttggtgt tgaagatcca
      241 gagaaaatga ttgaagattt ttggaatgca ctatatggaa gaagtaaagt tagtcaaaat
      301 attttatcaa ataaagatgt taaaattgtt aatattgaaa ataaagcgtg cattgaaatt
      361 catgtaccag aagcgcctta ttcgaagaaa ccgatatatg tagataataa aaaagattta
      421 gtatataaaa gagttgatga tgctgataga attgcgactg aagaagagta taaattcatg
      481 attgtaaatt ctcaagacga tatagataca gaattattag ataactatga catgtctgat
      541 ttaaatcacg aatctatcga aaattatagg aaacttctat taaaaaatac taatgatgag
      601 agatatgcga atatgagcca actggattta atgatagatt taggagcata tagaaaagat
      661 agaagttcga aagacaaaca gtataaaatg actacagcat gtttattatt ctttggtaag
      721 tataatgcga ttagtgatag attcccagga tttcaattag attattttaa gaaaacaaat
      781 tacctagata ctgattggaa agatagaata tcaagtggag atttaggtaa tgaagattta
      841 aacgtgtata gtttttttga aaaagtattg ataaaattaa ctgataacat tgaggaatca
      901 tttagcctaa atgatggttt gactagacaa aattatgcaa gagatttaaa agtagcaatt
      961 cgcgaagcac tggttaatac attaatgcat gcgtattatg atactaagca aagtattaaa
     1021 atagttaatt gtgaagattt tatagagttt tataatccgg gtaatatgag aataaataaa
     1081 gaagatttta ttcatggagg gcattcaaag gacagaaata gtatattatc gacgcttttc
     1141 agaagagtag gatattcaga aaaagctgga tctggaggac caaggatatt cgatgtagtt
     1201 aatagacata agcttaaaac gcctgaaata gaattaacgg acatggacac taatgtagta
     1261 ctttggaaac aagatttaat gaaggagttt gaaaaatatc ctgagttaga caaaaaagta
     1321 ataaagtata ttattgacta tggatcaata agtaagggtg aagccttaaa aatggaaaat
     1381 atgacagaat atcagtttag aaatatttta aaaaaactaa aagatgataa cttgataaaa
     1441 aaagaaggtg aaggtccggc tactaaatat gtgttaatag aatcaaaaga agctgatata
     1501 ttgcgaacta aaaaagtaat taaaagttta gagtctttct ttaggaataa ataaaaaaac
     1561 aagagatagg tgcgaagtgt ttggattata caaatgcttc gcatcttata tttattaata
     1621 aatatcatag aaaaccgtat cattaaccga tacgcagaga tgcggttttt tagacacttc
     1681 ataaagggat tttgaacgta tcagaacata tgaggtttat aggaattgct gttatgtttt
     1741 ttgatcacat caataaacaa aaaaggtatg tactatgtaa aatatttatt aaatgatata
     1801 aagcgagggt atataaatga tttttaaata gatatttatc caataatata aaaaggaact
     1861 ataagctata tctaaaagct atatctaact acttatagtc cttcttcatt agtataaata
     1921 taattattaa gaaaggtata tatctttgta accttcgttt acattaataa tgttgtgata
     1981 acctttgtgt tctaaaatac caatagctat agaacttcta atgccagact gacagtctac
     2041 ataaataaca tcgtttctat tgaaaggtaa atctgtttct aaaagtttgc cgtgtggcac
     2101 atgaaccgct tgggataagt ggccattatt ccattcatta tcattacgta catctaagac
     2161 atgtgcttca ttaccagtta tgtctttact atgaacagat tgtgtttgaa tttgagcttg
     2221 tggtaactga tatccagaca cattatcata tccaataagt tgtaaagtat gttatgtgtt
     2281 gcttttgaaa caaggtgata gtctccaatc aagttaattt cttgattata gtttagatac
     2341 cagccaattt gattgatgaa atttttatca tatggaatat tgattgtacc ttcaatatgt
     2401 ccaccatgat aagcctcctt actgcggaga tcaaaagtta atctgtttgt acttgtagct
     2461 ggataaaccg tataaggttg atataaattc ataccgaatt gattaatttt tttcatttgt
     2521 gcaaaatgat gtggtggtgc aggttggtca gaaatgagtt tatcgataaa ggtagcttca
     2581 ttattttcag aaaaagccca gttcgtttgt ttttcatagc caagcgtaga tgttggaata
     2641 gcacctaaag atttaccaca aggactacca gcgccatgac caggccaaat ttgaatgtag
     2701 tctggcaagt ctttaatact ttcaatagat ttaaacattt gttttgcgcc tatttcagat
     2761 aatccttcta ctttaacagc tttttctagt aaatcaggtc taccgatatc tcctacaaaa
     2821 ataaaatcac cactgaatag tcccattgga acttgtgctc cagcaccttc gtcagtaagt
     2881 aaaaaactta tactttctgg cgtgtgacca ggtgtatgaa gcacttttaa ttttatattt
     2941 cctacataaa tatcatcatt atgttgaaca aaatgagtgt ggttaggcat atttttataa
     3001 cctaacatgt catcactttc gcccgataca taaatactag catttaactt tatagcaaca
     3061 tctctaattc ctgaaacaaa atttgcatgt atatgtgttt cagctgcatg agtaatggtt
     3121 aaactctctt catcggcaac tcgaatatat gaagataagt cacaaatagg atcaatgatc
     3181 atggcttctc cagttttttg acaaccgatt aaataagatg cttgagataa atgtttatca
     3241 taaattgatg atagtcaatt tgttatcaag ggtgataatt atattaacta tggtaattac
     3301 atggaattaa agttattaaa caaacttgat gatgaccaat tagatttaga atttttacaa
     3361 gataatgggt taaaaggtaa tgttcaaggt cctatgactt taaaggaacc tttaaaaagt
     3421 tatatacaaa agtcttgaaa acgaatattt taaatttaga taatgagtta gaaaacactg
     3481 aatatgtcga aggtaaacca tatgtacagt acggtattaa atatgaaaga aatcaggcat
     3541 taagaaatga agctattaaa attcatggaa ctacatgtaa agtatgtgga tttgatttta
     3601 aagctaagta tggcgattta ggtgagggtt ttattgaaat tcatcattta aaaccaatgt
     3661 tttcaataaa aagagaaata aaagtaaatc cacaaaaaga tttagtccca ctatgttcta
     3721 attgccataa aatgattcat agaaatacta aaaaaccttt aacgattaaa gaattaa
//
LOCUS       DALAGD010000157         3720 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.162, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000157 DALAGD010000000
VERSION     DALAGD010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.162"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            193..699
                     /locus_tag="O2701_002651"
     CDS             193..699
                     /locus_tag="O2701_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294511.1"
                     /translation="MIIKILTILLLLCILSYLVTNRKKPFLFLKTLFMGVVFIFIGYI
                     SLAISAVIIYGIIQFITIDFGSFFLMGIILILISSIFQLFIVRLLFRKKNVDLTEVVV
                     LEHLIQWFLVYFAIYQAVNEKMDINDINIDNFQSVFFDVSNLNLVILPTLIISWVTIF
                     NYRMRSYK"
     gene            958..1455
                     /gene="efb"
                     /locus_tag="O2701_002652"
     CDS             958..1455
                     /gene="efb"
                     /locus_tag="O2701_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement convertase inhibitor Efb"
                     /protein_id="HCZ4294512.1"
                     /translation="MKNKLIAKSLLTLAAIGITTTTIASTADASEGYGPREKKPVSIN
                     HNIVEYNDGTFKYQSRPKFNSTPKYIKFKHDYNILEFNDGTFEYGARPQFNKPAAKTD
                     ATIKKEQKLIQAQNLVREFEKTHTVSAHRKAQKAVNLVSFEYKVKKMVLQERIDNVLK
                     QGLVK"
     gene            1609..1959
                     /gene="scb"
                     /locus_tag="O2701_002653"
     CDS             1609..1959
                     /gene="scb"
                     /locus_tag="O2701_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN-B"
                     /protein_id="HCZ4294513.1"
                     /translation="MKFKKYILTGTLALLLSSTGIATIEGNKADASSLDKYLTESQFH
                     DKRIAEELRTLLNKSNVYALAAGSLNPYYKRTIMMNEYRAKAALKKNDFVSMADAKVA
                     LEKIYKEIDEIINR"
     gene            complement(2612..2797)
                     /locus_tag="O2701_002654"
     CDS             complement(2612..2797)
                     /locus_tag="O2701_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294514.1"
                     /translation="MNKHINKKAAKLLEDYKKVQQRKSKETDRGCLKSVIMLIVLFFL
                     IIFGIVACSTNVNFFFQ"
     gene            complement(3419..3652)
                     /locus_tag="O2701_002655"
     CDS             complement(3419..3652)
                     /locus_tag="O2701_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294515.1"
                     /translation="MVFNDWEINVVYSGEHEVVTNEKSLFVIDEFYDVAIAIYYLDGK
                     LKVAHVNYGSDFTIDVANKVFELNIQNPNVDEH"
BASE COUNT         1396 a          486 c          547 g         1291 t
ORIGIN      
        1 tatttaattg aattatgcat tttcgaagta aacataattc aacacttaca tttttaaata
       61 gataaaatag tgtaaaatca cgtgccaaga ttactataga gtaggcattg agtattattg
      121 gcaatcactt aagtgattgt tatttttcgt ggtcaataat aaagatataa ataaatatat
      181 tgaggtcaaa cgatgataat taaaatttta acaattctat tactactttg tatattgagc
      241 tatttggtta caaatagaaa gaagcctttt ctgttcttaa agacactctt tatgggtgtg
      301 gtatttatct ttataggata tatttcactg gcaatatctg ccgtaattat ttatggtatt
      361 attcaattta tcacaattga ttttggtagt tttttcttaa tgggtattat attgatcttg
      421 atttcaagta tattccaatt atttatagtt agattacttt ttagaaaaaa gaatgtcgat
      481 ttgacagagg ttgtcgtttt agagcattta attcaatggt tcttagttta ctttgcgatc
      541 tatcaagcag taaatgaaaa aatggacatt aatgatatta atatcgacaa tttccaatct
      601 gtcttttttg acgtgtctaa tttgaattta gtaattctac caacgttaat cattagctgg
      661 gtcacaatat ttaactatag aatgagaagt tacaaataaa atctatgaga ttataccttc
      721 agacaccaac attcaaatgg tgtctttttt gttgtgtggt tttatttttg aaattcgaaa
      781 aagtagaggc atgaattttt tgactagtgt ataagtgctg atgagtcaca agatagatag
      841 ctatattttg tctatattat aaagtgttta tagttaatta ataattagtt aatttcaaaa
      901 gttgtataaa taggataact taataaatgt aagataataa tttggaggat aattaacatg
      961 aaaaataaat tgatagcaaa atctttatta acattagcgg caataggtat tactacaact
     1021 acaattgcgt caacagcaga tgcgagcgaa ggatacggtc caagagaaaa gaaaccagtg
     1081 agtattaatc acaatatcgt agagtacaat gatggtactt ttaaatatca atctagacca
     1141 aaatttaact caacacctaa atatattaaa ttcaaacatg actataatat tttagaattt
     1201 aacgatggta cattcgaata tggtgcacgt ccacaattta ataaaccagc agcgaaaact
     1261 gatgcaacta ttaaaaaaga acaaaaattg attcaagctc aaaatcttgt gagagaattt
     1321 gaaaaaacac atactgtcag tgcacacaga aaagcacaaa aggcagtcaa cttagtttcg
     1381 tttgaataca aagtgaagaa aatggtctta caagagcgaa ttgataatgt attaaaacaa
     1441 ggattagtta aataaaactt caatcgttgc tgttatctgg aaataattaa ttaaatgtta
     1501 tgttaatttt tgttaatgaa aaaagtaatc tatttaatga caggttaatg taattgtcct
     1561 gaaattgact atatactcag taagtatcaa ttttaaggag agcttataat gaaatttaaa
     1621 aaatatatat taacaggaac attagcatta cttttatcat caactgggat agcaactata
     1681 gaagggaata aagcagatgc aagtagtctg gacaaatatt taactgaaag tcagtttcat
     1741 gataaacgca tagcagaaga attaagaact ttacttaaca aatcgaatgt atatgcatta
     1801 gctgcaggaa gcttaaatcc atattataaa cgtacgatta tgatgaatga atatagagct
     1861 aaagcggcac ttaagaaaaa tgatttcgta tcaatggctg atgctaaagt tgcattagaa
     1921 aaaatataca aagaaattga tgaaattata aatagataat aaataaaaca ggttgagaca
     1981 aaaaatggtc ttaacctgtt ttcaatttgc atatgtgata aattctatat caaaatgctt
     2041 atgtataatg aatgacattt aaaagtaggg gagacaaata taaatacaat agttcctagg
     2101 attactctca aaataactat atcaattatt tactttgctc tcctattttt taaaatatct
     2161 acatgtttaa acaatcaaaa gtgtacaata ttaaattatc atttccagtt ctagtgctat
     2221 attggtagta gttgactaaa tgaaaataag cttataacaa gttttttcaa tactcgtggg
     2281 gccacaacag agagaaatag gatcaccaat tccaacaaac aatgcaagtt ggcgggggcc
     2341 ccaatataga agctggcgaa aagtcagctt acaaaaatgt gcaagttggc ggggccccaa
     2401 catagagaaa ttggaacccc aatttctaca gacaatgcaa gttggggtag gacatcgata
     2461 aagaaatact ttttctttag caattagtat ttcttatgca tgagctttac tcatgtattc
     2521 attttttaag tacacattag ctacagctaa tgataaagaa ccactacata ataaatcatt
     2581 agtggttctt tatcatttct atctcactct tttactggaa gaaaaagttt acgtttgtag
     2641 aacatgccac aataccaaaa ataattaaga aaaataagac gataagcatg atgacacttt
     2701 tcaaacaacc tctatcagtt tctttcgatt ttctttgttg aaccttttta taatcttcaa
     2761 gtagttttgc ggctttttta tttatatgtt tattcatgat gttgactcct tataatatat
     2821 gtttaattca ttaaaatagt tgaaaacatg acttgaaata aagatataaa gctaaagtaa
     2881 ggatgttcaa ttgtttcaaa ttttatgaaa gtataacgtt aaatatgtta atttgatgat
     2941 gatatttgct ttttattttc caaatggaat ttacttaaac tgatgcatta aaatattaat
     3001 gaagcactag aatacataaa tgaatagtaa tggtgcacag tatagaataa ttaaggctat
     3061 attaagtata aatatcgtta actgtaagct atctttagtt ttaatataaa ctattaggat
     3121 aatcgacgta agaagaatca tatatattaa tgatgaagaa gtccatacaa aatccgcatc
     3181 atttgttgtt aataatggga ctataattaa tccgaaatta atcatgcatg ctatatatac
     3241 tataatgtta tacacaatgt taatttttgt tcaccacctt atacttctat tttaaaaact
     3301 tctttataat gatatattgt ttaatgttga aataattaga ttatctaatt ttcatttgct
     3361 ttacatgtaa aaggctatat atagtatgct ctttatgatt ctaaatgctt tttaatattt
     3421 aatgctcatc aacatttgga ttttgaatat tcaattcaaa aactttatta gctacgtcaa
     3481 ttgtaaaatc agaaccatag ttgacatgag ctacttttaa ttttccatct aaataataga
     3541 ttgcgattgc aacatcgtaa aattcgtcaa tgacaaataa actcttttcg tttgttacaa
     3601 cctcatgctc tcctgagtat acaacgttaa tttcccaatc attaaaaacc atttgttaac
     3661 ctccttgaac atttaaattg attcaactta agtttaactt attcatacaa cttcgtacaa
//
LOCUS       DALAGD010000158         3640 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.164, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000158 DALAGD010000000
VERSION     DALAGD010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.164"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            221..1456
                     /gene="arcA"
                     /locus_tag="O2701_002656"
     CDS             221..1456
                     /gene="arcA"
                     /locus_tag="O2701_002656"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCZ4294516.1"
                     /translation="MVQGPIQVNSEIGKLKTVLLKRPGKELENLVPDHLSGLLFDDIP
                     YLKVAQEEHDKFAQTLRDEGIEVVYLEKLAAESITEPEVRENFINDILTESKKTILGH
                     ETEIKEFFSKLSDQELVNKIMAGVRKEEIQLETTHLVEYMDDRYPFYLDPMPNLYFTR
                     DPQASIGRGMTINRMYWRARRRESIFMTYILKHHPRFKDKDVPVWLDRNSPFNIEGGD
                     ELVLSKDVLAIGISERTSAQAIEKLARNIFKDANTSFKKIVAIEIPNTRTFMHLDTVL
                     TMIDYDKFTVHAAIFKEENNMNIFTIEQNDGKDDIKITRSSKLRETLAEVLEVEKVDF
                     IPTGNGDVIDGAREQWNDGSNTLCIRPGVVVTYDRNYVSNQLLRDKGIKVIEITGSEL
                     VRGRGGPRCMSQPLFREDI"
     gene            1542..2963
                     /gene="arcD"
                     /locus_tag="O2701_002657"
     CDS             1542..2963
                     /gene="arcD"
                     /locus_tag="O2701_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HCZ4294517.1"
                     /translation="MDENKLGKTSLIGLVIGSMIGGGAFNIISDMGGQAGGLAIIIGW
                     IITAIGMISLAFVFQNLTNERPDLDGGIYSYAQTGFGDFIGFSSAWGYWFAAFLGNVA
                     YATLLMSAVGNFFPIFKGGNTLPSIIIASILLWGVHFLILRGVETAAFINSIVTVAKL
                     IPIFLVIICMIVVFNFSTFKSGFYGMTSGSVGVFSWGDTMAQVKSTMLVTVWVFTGIE
                     GAVVFSGRAKSKKDVGTATVIGLISVLVIYFLMTVLAQGVIQQNQISKLANPSMAQVL
                     EHIVGHWGSVLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNKAPV
                     NALIITNILVQLFLISMLFTDSAYQFAFSLASSAILIPYTLSAFYQVKYTIQNKSKAN
                     LKQWIIGIIASIYTIWLVYAAGLDYLLLTMLLYIPGLLVYSYVQRDNNKHLTKLDYTL
                     FIFIIVLAIIGIVRLITGNISVF"
     gene            3005..>3640
                     /locus_tag="O2701_002658"
     CDS             3005..>3640
                     /locus_tag="O2701_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCZ4294518.1"
                     /translation="MYEENIYIKNSEYEFDNNLKQLASYLNIPVSIVRPYKEDLTLYQ
                     YKKGQVIYHSTDQIKFVYFLVNGCILHESSNITGDNYLRLSKDENIFPMNFIFNETPA
                     PYEICTALTDCKILTLPKDLLEYLCRKHNEIFESLFKKLNETIQFQVEYIMALRANSA
                     KERIERILQILCLSIGDDNGEFYELKQIMTVQLISNLSGLNRKTTGEIIREL"
BASE COUNT         1305 a          481 c          647 g         1207 t
ORIGIN      
        1 agtaaaaaat ctttttgctt gaataagata aggttttaac aatgagattt ttcaaattat
       61 tcactattat atagtacaaa atacatattc atacaaaatt ttgcattgaa taaatctata
      121 tatgtgaaaa ccctccctaa acaattgtga attaaatcac acgatgtttt ttgaaagcgt
      181 tttataatgt gaatgtaata aagcaaagga ggaatttgaa atggtacaag gacccattca
      241 agtaaatagt gaaataggca aattgaaaac tgtgttgtta aaaagaccag gaaaagaatt
      301 agaaaattta gtacctgatc atttaagtgg tttattattc gatgatattc cctacttaaa
      361 agttgcacaa gaagagcatg acaaatttgc tcaaactttg agagatgaag gaatcgaagt
      421 agtttattta gaaaaacttg cagcagaatc tattactgag ccagaagtac gcgagaactt
      481 cataaacgac atattaacag aatctaaaaa gacaatatta ggtcatgaaa ctgaaattaa
      541 agaattcttt tcaaagttat ctgaccaaga acttgtaaat aaaatcatgg ctggcgtacg
      601 taaagaagaa attcaacttg aaacaaccca tttagtagaa tatatggatg atagatatcc
      661 attttactta gatccaatgc ccaaccttta ttttacaaga gatccccaag cttcaattgg
      721 tagaggaatg acaattaaca gaatgtattg gagagcacga cgtagagaat ctatttttat
      781 gacatatata ctgaaacatc atccaagatt taaagataaa gatgtaccag tatggttaga
      841 tcgtaactca ccatttaata ttgaaggtgg agatgaatta gtattatcga aagatgtttt
      901 agctattggt atatcagaac gtacatcagc tcaagcaata gaaaagttag cacgtaatat
      961 tttcaaagat gcaaacacaa gttttaaaaa aatcgtagct attgaaatac ctaatacacg
     1021 tacatttatg cacctagata cagtactaac tatgattgac tacgataagt ttacagtaca
     1081 tgcagcaata tttaaagaag aaaataatat gaatatattt accatagaac aaaatgatgg
     1141 taaggacgat ataaaaatta ctcgttctag caagttacgt gaaacacttg ctgaagtttt
     1201 agaagtagaa aaagtggact ttattccaac aggtaatggc gacgttattg atggtgcacg
     1261 tgaacaatgg aatgatggct caaacacatt atgtattcga ccaggggttg tggtgacata
     1321 cgatcgcaac tatgtatcaa accaactttt acgcgacaaa ggaattaaag tgattgaaat
     1381 tactggtagt gaacttgtac gtggacgcgg aggcccaaga tgtatgagtc agccgttatt
     1441 tagagaagat atttaaattt gaaaggtggt aaaagtcaca agtaattcat tcattaaatc
     1501 aaataacaag atttttacca cctttattag gggagattaa tatggatgaa aataaattag
     1561 gtaaaacttc cttaattggt ttagtcatag gctctatgat aggcggtggt gcattcaata
     1621 tcatctcaga tatgggtggc caagctggtg gacttgcaat aattatcggt tggataataa
     1681 ctgctattgg tatgatttct cttgctttcg tatttcaaaa tttaacaaat gagcgaccag
     1741 atcttgatgg aggaatttat agttatgctc aaacagggtt tggagatttt attggttttt
     1801 caagtgcttg gggatattgg tttgcagcat ttctaggtaa tgtggcttat gcaaccctat
     1861 taatgtcagc tgtgggtaac tttttcccta tatttaaagg aggtaacaca cttccaagta
     1921 ttatcatagc atcaatttta ttatggggtg tacatttttt aatacttaga ggtgtagaaa
     1981 ctgcagcgtt tataaatagt attgttacag tagctaaatt aatacctata tttctagtta
     2041 ttatatgcat gatagttgta ttcaacttca gtacttttaa atccggtttt tatggtatga
     2101 ctagtggaag tgttggcgtt tttagttggg gagatacaat ggcacaagta aaaagtacta
     2161 tgttagtaac tgtatgggta ttcacaggga ttgaaggagc cgttgtcttt tctggacgtg
     2221 caaagtctaa aaaggatgta ggaactgcta ccgttattgg tttgatttct gtgctagtca
     2281 tttatttctt aatgactgta ctagcccaag gtgtcattca gcagaaccaa atttcaaaac
     2341 ttgctaatcc atcaatggca caagtattag aacatattgt aggtcattgg ggttcagtgt
     2401 tagttaatat aggcttaatt atctctgttt taggagcttg gttaggatgg acattactag
     2461 ctggtgaatt accattcatt gtagctaaag atggactttt cccgaaatgg tttgctaaag
     2521 aaaataagaa taaagctccg gtcaacgctt taattattac taatatatta gttcagttat
     2581 ttttaattag tatgttgttt acagatagtg cctatcagtt tgcgttttca cttgcatcaa
     2641 gtgcaatctt aattccatat acactcagtg ctttttacca ggttaaatat actattcaaa
     2701 ataaatctaa agctaattta aaacaatgga taataggaat tattgcatct atttacacaa
     2761 tttggttggt ttatgcagct ggattagatt atttactatt aacgatgttg ttatatatac
     2821 ctggattact cgtatacagc tacgtacaaa gggataataa caaacatttg acaaaattgg
     2881 attatacgtt attcatattc atcattgtac ttgcaataat aggaatagtt cgtttgatta
     2941 caggtaatat ttctgtattt taagttatga tttaaactaa gtaattttta aggggtttat
     3001 agttatgtat gaagaaaata tttatattaa aaattcagaa tatgaatttg ataataatct
     3061 taaacaatta gcatcatact taaatattcc tgttagtatt gttagacctt ataaagagga
     3121 tttaacactt tatcaatata aaaaaggaca agtcatatat cattcaactg atcaaataaa
     3181 atttgtatac tttttagtaa atggttgtat tttacatgaa tcttctaata ttactggtga
     3241 caattattta agattaagta aagacgaaaa tatatttcca atgaacttca tatttaatga
     3301 aacccctgca ccatatgaaa tatgtacagc tttgacagat tgtaaaatat taactttacc
     3361 gaaagattta cttgagtatt tatgtagaaa gcataatgaa atatttgaaa gtctcttcaa
     3421 gaaacttaat gagactattc aatttcaagt agaatatatt atggcgttaa gagctaattc
     3481 agctaaagaa agaattgaaa gaatactaca aattttatgc ctttcaattg gggatgataa
     3541 tggagaattc tatgaattaa aacaaattat gactgttcaa ttaataagta atttatctgg
     3601 acttaacaga aaaactactg gtgaaataat cagagaatta
//
LOCUS       DALAGD010000159         3601 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.165, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000159 DALAGD010000000
VERSION     DALAGD010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.165"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..297
                     /locus_tag="O2701_002659"
     CDS             <1..297
                     /locus_tag="O2701_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCZ4294519.1"
                     /translation="PQKVVTDQAPSTKAAMAKIIKAFKLKPDCHCTSKYLNNLIEQDH
                     RHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEISIMLAS"
     gene            355..732
                     /locus_tag="O2701_002660"
                     /pseudo
     CDS             355..732
                     /locus_tag="O2701_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            729..1610
                     /locus_tag="O2701_002661"
                     /pseudo
     CDS             729..1610
                     /locus_tag="O2701_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143419.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            1726..2655
                     /locus_tag="O2701_002662"
     CDS             1726..2655
                     /locus_tag="O2701_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4294520.1"
                     /translation="MKNLNNIIEINNLTKQYGSQIAVNNLSITVESGQIYGFLGRNGA
                     GKTTTIRMLLGLIKPTKGNIKIFEKEFESNKTEILKNIGAMVETPGFYSNLTAKENLT
                     INAKILGIQKENAIEEVLEIVGLASEKVKLFKNYSLGMKQRLGIARALLHNPELLILD
                     EPTNGLDPIGIKEIRKLIKDLAQSRNITILISSHILSEIEQLVDKVGIIHNGVLLEEI
                     MYEDLKKKNRQFISISVSDEEKVACLLETNFGIYDYKIKENGEFKIYSHIDRQQEINR
                     LLVLNNIDVFQMRVIEDNLETYFEDLVGGGTIG"
     gene            2648..3385
                     /locus_tag="O2701_002663"
     CDS             2648..3385
                     /locus_tag="O2701_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4294521.1"
                     /translation="MDNLLRTEFIKLKRSYMFLISILGTCVAPFMVVVASFISMRTKV
                     PTPNIVFKQLFEEVSLYHMLIIGPPLYGVIIAYLFGREYIEDTLKNLLIIPISRESFL
                     VSKIILLFLWIQTLSVIAWLLTLMLGMLLHFQGLSQSLIIDSFFTFVIGGVLLWILSP
                     PIIILSIYFKNYVPTIVLTIIITLINLMTANSEHRGLFPWAAVGDISRGTLPNTYPFL
                     LSLIIIIVTSIFSVIILFSYFKKTDIS"
     gene            3399..>3601
                     /locus_tag="O2701_002664"
     CDS             3399..>3601
                     /locus_tag="O2701_002664"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499250.1"
                     /note="BacA; phosphatase activity in Escherichia coli not
                     kinase; involved in bacitracin resistance as bacitracin
                     supposedly sequesters undecaprenyl disphosphate which
                     reduces the pool of lipid carrier available to the cell;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase"
                     /protein_id="HCZ4294522.1"
                     /translation="MGFIEQIKAFILGVVEGLTEFAPVSSTGHMIIVDDMWLNTADDL
                     GSASATTFKVVIQLGGSVAKLNL"
BASE COUNT         1316 a          493 c          638 g         1154 t
ORIGIN      
        1 cctcaaaagg tagttacaga tcaggcacct tcaacgaagg cagcaatggc taaaataatt
       61 aaagctttta aacttaaacc tgactgtcat tgtacatcga aatatctgaa taacctcatt
      121 gagcaagatc accgtcatat taaagtaaga aagacaagat atcaaagtat caatacggca
      181 aagaatactt taaaaggtat tgaatgtatt tacgctctat ataaaaagaa ccgtaggtct
      241 cttcagatct acggattttc cccatgccac gaaattagca tcatgctagc aagttaaacg
      301 aacactgaca tgatatatta gtgattagct atatttttta ctttgcaaca gaaccagcca
      361 tttaatatga ctgttacaat tactaagatt caagcactat tacgccgcac gtatgatttc
      421 accactcaag ttaataattt aacagtagat gattgcgagt tactacttga tgcagcaaag
      481 cttaaattta atgaagaaac catagacctg acacacactg aattgcaaat catgcatagc
      541 ttattccaga acaagggcaa attcgtcagt cgtaatgcgt taatcgaaaa atgctgggaa
      601 tcagaacact tcattgatga caatacgctt gctgtcaaca ttacacgttt acgcaaaaag
      661 ctacaaaaac taggacttac agattttatc gaaacgaaga aaaatgtcgg atatcgtgta
      721 ggtgacgtat gaatttcttg aaatcaatcc aacgtgaaat ttatattgtc atccttactt
      781 gtgctttatt ctactttata ttattcatgt ataatgtgcc gatgcaagca tatatgattg
      841 gtctaggtat tgtattgttt gtttcaatga tttactgggg tatccattac atgaacttta
      901 aaaaagaata aacactacag gaacagaacg aagatttaag aatcgaaaac cataaacttc
      961 gtaatgactt tattaactac caaagtgagt tagagtcata ttttttaatg tgggtgcatc
     1021 aaatcaagac accgattaca gcatcaaagt tactgcttga agatcctgat gaacacgtga
     1081 tttctaacgt gcgtaatgaa gtcttacaaa ttgataatta cacgaatctt gcactgaact
     1141 atttgaaact catgaaccaa aagacataca tggtctttat ggaagtgagt gtagatgaat
     1201 taattcgacc actgattaaa cgttatgcga tacagtttat tcatcatcaa acaaagttgc
     1261 attatgagaa aagtgaagcg gttatattaa cagatgccaa atgggcatca attatgattg
     1321 agcaaatatt aaacaatgca ttaaaatatg cacgtggcaa agatgtgtgg attacgtttg
     1381 acgaaaatac aaagaaatta tcaatccgag ataacggtat cggcattaat gcgagtgatt
     1441 taccgaaaat ctttgataaa ggtttctcgg gctataacgg tagaataagt gagaaatcaa
     1501 gcggtattgg tttatatatt gtcagcacaa tctcaaagcg cttaggccat aaagtaagtg
     1561 ctacatctac attaaacgat ggttcaatat tcaccattca atttaaataa ctccaatctt
     1621 tcatttttgt aagattagcg gctgtttttg taagaataga taaaggcagc cttttctaat
     1681 cgcgcgtaag ataaaggtaa caacaaacta agaaaggtgc cagaggtgaa aaatttgaat
     1741 aatataattg aaataaataa cttaacaaag caatatggaa gtcagatagc agttaataat
     1801 ttgagtataa ctgttgaaag tggacaaatt tatggttttt taggaagaaa tggtgcaggc
     1861 aaaacaacta ctataagaat gcttttaggc ttaattaaac ctacgaaagg taatattaaa
     1921 atatttgaaa aagagttcga aagtaataaa actgaaattt taaaaaatat tggtgctatg
     1981 gttgaaacgc caggttttta tagtaatttg acagctaaag agaacttaac tataaatgct
     2041 aaaatattag gtatacaaaa agaaaatgca attgaagaag tgttagaaat agtaggacta
     2101 gcttctgaaa aagtaaaatt gtttaaaaat tattctttag gtatgaagca acgtttaggg
     2161 attgcgagag ctttattgca caatcccgaa ttattgatac ttgatgagcc gacgaatgga
     2221 ttagatccta ttggcataaa agaaattaga aagttgatta aggatctggc tcaatctaga
     2281 aatataacca ttttaatttc aagtcatata ttgtcagaaa tagagcagtt agtagacaag
     2341 gtgggtatta ttcataacgg agttttgtta gaagaaatta tgtatgaaga tttaaaaaaa
     2401 aagaacagac aatttataag tatatcagta tcagatgaag aaaaagttgc atgtttgttg
     2461 gaaactaact tcggaattta tgactataaa attaaagaaa atggcgaatt taaaatttat
     2521 tcacatattg atagacaaca agaaattaat agattattag tattaaacaa tattgatgtt
     2581 ttccagatga gagtgataga agataatcta gaaacttatt tcgaagactt agtaggaggt
     2641 ggcacaattg gataatctac tccgtacaga atttattaag ttaaaaagat catatatgtt
     2701 tctaataagt attttaggta catgcgtagc accttttatg gtggttgtag cttcttttat
     2761 tagtatgcgg acgaaagtac cgactcctaa tattgtattt aaacagttat ttgaagaagt
     2821 cagtttatat catatgctaa ttattggacc tcctctatat ggggttataa tagcctattt
     2881 gtttggaaga gaatatatag aagatacttt aaaaaactta ttaattatac ctatatctag
     2941 agaaagtttt ttagtaagta aaataatatt attattcttg tggattcaaa cactatctgt
     3001 tattgcatgg ttgctaactt taatgctagg tatgctattg cattttcaag ggttaagcca
     3061 gagtttaata attgattcat tctttacatt tgttattggc ggagtgttat tatggatatt
     3121 atctccacca ataattattt tgtcgatata ttttaaaaat tatgttccca caattgtttt
     3181 aaccataata attactttga taaatttaat gactgctaat tctgaacata gaggattatt
     3241 tccttgggct gcagttggag acatatctag aggaacatta cctaatactt atccatttct
     3301 attatcttta attatcataa ttgttacatc tatattcagt gtaattatat tattctcata
     3361 ctttaaaaaa actgatataa gttaaaagga ggagaaaaat gggttttata gagcagatta
     3421 aagcatttat tttaggtgta gttgaagggt tgacagaatt tgctccagta tcatcaacag
     3481 gtcatatgat tattgttgat gatatgtggt taaacactgc tgacgattta ggaagtgcgt
     3541 ctgcaactac ttttaaggtg gtaatacagt taggaggttc tgttgcaaag ttgaatttat
     3601 a
//
LOCUS       DALAGD010000160         3507 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.166, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000160 DALAGD010000000
VERSION     DALAGD010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.166"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(87..1223)
                     /locus_tag="O2701_002665"
     CDS             complement(87..1223)
                     /locus_tag="O2701_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HCZ4294523.1"
                     /translation="MTENKSFKESHPLDDFISDKELSNTTIQKEKLTIEQQKQVDTIS
                     KQINPLDNEGLLAFGSDLQKQMSQFSHQMLDEVQSKDVGPIGDTLSDLMSKLKSVNPN
                     ELNTDKPSMLKRIFSRAKSSINEIFSRMQSVSAQVDRITIQLQKHQTHLTRDIELLDT
                     LYDKNKQYFDDLSLHIIAAQQKKLQLENEKLPQLQQQAQQSTNQMDIQQVADMQQFID
                     RLDKRIYDLQLSRQIALQTAPQIRMIQNVNQALAEKIQSSILTSIPLWKNQMAIALTL
                     MRQRNAVAAQRAVTDTTNDLLTANAEMLKQNAIETATENERGIVDLDTLKRTQRNIIE
                     TIEETLIIQQHGREERQLAEKELQQLEQDLKSHLVNIKGPNKQS"
     gene            complement(1255..1884)
                     /locus_tag="O2701_002666"
     CDS             complement(1255..1884)
                     /locus_tag="O2701_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HCZ4294524.1"
                     /translation="MTVRYNISHIFGVLVGIPVAFLTSIFGMIALDVSFLIDMSIGIV
                     GFLMTYLPIQKLTSRKYLNEIGLTRKDYRYIRNQLNHTHQKLRGILKTYVNIRSIKDF
                     RQINDIYQISRSIYTTVRQRPASFYKVEGFFYSHIDNALNLVDAYTRLAKMPKKSINE
                     QQKLEQTRITLDEVKRTLIADLKRLNEDDYERLDIEMELNKLHQKHHQD"
     gene            complement(1903..2172)
                     /locus_tag="O2701_002667"
     CDS             complement(1903..2172)
                     /locus_tag="O2701_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCZ4294525.1"
                     /translation="MRHIHLQVFGRVQGVGFRYFTQRIAMNYNIVGTVQNVDDYVEIY
                     AQGDDADIERFIQGVIEGASPASNVTSHQLEELELNQKLSDFRSI"
     gene            2335..2643
                     /locus_tag="O2701_002668"
     CDS             2335..2643
                     /locus_tag="O2701_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HCZ4294526.1"
                     /translation="MWTVTKIRADYEGWWLFSDWPENIVEKYQYQDFDDMFKHYQQLI
                     NQCKVQFDNYVTGKYNIYAFYNNCDMNYCEDCEEDLQIFYSFIVLQNNEVYYKLPIID
                     "
     gene            2814..3014
                     /gene="cspA"
                     /locus_tag="O2701_002669"
     CDS             2814..3014
                     /gene="cspA"
                     /locus_tag="O2701_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspA"
                     /protein_id="HCZ4294527.1"
                     /translation="MKQGTVKWFNAEKGFGFIEVEGENDVFVHFSAINQDGYKSLEEG
                     QAVEFEVVEGDRGPQAANVVKL"
     gene            3211..>3507
                     /gene="msa"
                     /locus_tag="O2701_002670"
     CDS             3211..>3507
                     /gene="msa"
                     /locus_tag="O2701_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarA expression modulator protein Msa"
                     /protein_id="HCZ4294528.1"
                     /translation="MKYLILSLVANLLVFGVLSAIGLNINILAAMMIVLVIPIMISGI
                     LFFKTNIDKTYIFFNIIFIDFYYYIYNVHLMTLPKFNNYIKAEMMELEDIDVLIT"
BASE COUNT         1096 a          513 c          550 g         1348 t
ORIGIN      
        1 taatagaaat cagctttttt acattgccta agaactttaa ggaacttaat gtcccaagct
       61 cttttttgtt atatctaatt cgtaatttat gattgtttat tcggtccttt gatgttcact
      121 aaatgtgact ttaaatcttg ttctaattgt tgtaattctt tttcagctaa ttgtcgttct
      181 tcgcgaccgt gttgttgaat aattaatgtt tcttcaattg tctcaataat gttacgctgt
      241 gtacgtttca atgtatcaag atcaacaatg ccacgctcat tttctgttgc agtttcaatc
      301 gcattttgtt tcaacatttc agcatttgct gttaataaat cattagttgt atcagtgaca
      361 gctcgttgtg cagcaactgc attacgctgt ctcattaatg taagcgcaat ggccatttga
      421 tttttccata gtggaatact tgtcaaaatt gaactttgta tcttctcggc aagtgcttga
      481 ttaacatttt gaatcatacg aatttgtggc gcagtttgca aagctatttg tcttgaaagc
      541 tgtaagtcat atatgcgttt atctagtcta tctataaatt gctgcatatc tgcaacttgt
      601 tgaatatcca tttgattagt ggattgctgc gcttgctgtt gcaattgtgg tagcttttca
      661 ttttctaatt gcaacttttt ttgctgtgca gcaatgatat gcaatgataa gtcatcaaag
      721 tattgtttgt ttttatcata tagcgtatct aataattcaa tatctcttgt taaatgtgtt
      781 tgatgtttct gcagttgaat cgttatgcga tcgacttgag cactaactga ttgcattctt
      841 gaaaagattt cattgataga cgactttgct ctgctaaaaa ttctttttaa catagatggt
      901 ttatcagtat ttaactcatt tggattaact gactttagtt ttgacattag atctgacaaa
      961 gtatctccaa taggaccaac atctttactt tgtacttcat ccaacatttg atgtgaaaat
     1021 tgagacattt gtttctgtaa atcagaacca aacgctaata aaccttcatt gtctaaaggg
     1081 ttaatttgtt tactgattgt gtctacctgt ttttgttgtt caattgttaa cttttctttt
     1141 tgaatagtag tattcgataa ttctttatcg cttataaaat catctagtgg atggctttct
     1201 ttgaaacttt tattttcagt catcaatcat ctctccaata gttgctattt tacatcaatc
     1261 ttgatgatgt ttttgatgta acttatttaa ttccatttca atatctaaac gttcataatc
     1321 atcttcgttg agacgcttta aatcagcgat taatgttcgt ttgacctcat ccaaagtaat
     1381 tcgtgtttgt tctaactttt gctgttcatt aattgatttt ttgggcattt ttgctagacg
     1441 tgtatatgca tcaaccaaat ttaaagcatt atcaatatga gaataaaaaa agccttcaac
     1501 tttataaaat gatgcaggtc tctgtctaac tgtcgtataa atagaacgtg aaatttggta
     1561 tatatcatta atctgcctaa aatctttaat tgatcttata ttgacatacg tttttaaaat
     1621 acctctaagt ttttggtgtg tatgatttaa ctgatttcga atatagcgat agtcttttct
     1681 agtcaaacca atttcgttta aatatttgcg tgaagtgagt ttttgtatcg gtaggtatgt
     1741 cattaaaaag ccaacaatac caatagacat atcaattaaa aaagatacat caagtgcaat
     1801 catcccaaat atgcttgtta aaaacgctac aggaattccc actaacaccc caaatatatg
     1861 agaaatatta tatctcactg tcatcttcct ttatttagca ttttatattg atcgaaaatc
     1921 cgataatttt tgatttagtt ctaactcttc aagttgatgg cttgttacat ttgatgctgg
     1981 tgaggcacct tcaattacac cttgaataaa tctctctata tctgcgtcat ccccttgtgc
     2041 atatatctct acatagtcat ctacattttg aacagtaccg acaatgttat agttcattgc
     2101 aatgcgttgt gtaaaatatc taaatccgac gccttgaacg cgtccgaata cttgtaaatg
     2161 tatatgtctc atttttacca cctcataatg ttattatacg tagttttact taaaaaaact
     2221 aataattact atagttacta ctttgtttgt ttcaagtcgt caaacttgat tttcagagga
     2281 taaaggtata aaaataagta tagagttttt gaagtatgga aggggtcttt aataatgtgg
     2341 acagttacca aaattagagc cgattatgag ggatggtggt tattcagtga ctggccagaa
     2401 aacattgttg aaaaatatca atatcaagat tttgatgaca tgtttaagca ctatcaacaa
     2461 ttgattaatc aatgtaaagt tcagttcgat aactatgtca caggcaaata taatatttat
     2521 gcattttata ataattgtga catgaactat tgcgaagatt gtgaagagga tttacaaata
     2581 ttttatagtt tcattgtttt acaaaataat gaagtatatt ataaactacc aataattgat
     2641 taaatgaata ttttttatta ttgtattgca atttggtaat aactgcacta taatacaagt
     2701 taagcagatg attattccat attgcaaaga atatttgagt gaaatttgca tgtatttttt
     2761 gtaatatgcg aataagcata ttgaatgaat tttagtcttg gaggtttcag attatgaaac
     2821 aaggtacagt taaatggttt aacgctgaaa aaggattcgg ctttatcgaa gttgaaggag
     2881 aaaatgacgt attcgtacat ttttcagcaa ttaaccaaga tggttacaaa tcattagaag
     2941 aaggtcaagc tgttgagttt gaagtagttg aaggcgaccg cggtccacaa gctgcaaacg
     3001 ttgttaaact ataattctta gatttgaatc attgatttta actaacacct taccaagaag
     3061 taaggtgttt ttatatgctt attacaaata cttaacgcga tgagtttctc aaccaaattg
     3121 agaactcttt ttttatatca ccaacattgt ttgcaataga aaaatatatt tatataatat
     3181 aattatggtg tacaacgacg aggtgatttt atgaaatatc ttatccttag tttagttgct
     3241 aatttattag ttttcggtgt attatctgca attggattaa atattaatat tttagcagca
     3301 atgatgattg tattagtgat tccgataatg atttctggga tactattttt caaaactaat
     3361 attgataaaa catacatatt ttttaacatc atatttatcg acttttatta ctatatttac
     3421 aatgtacatt taatgacttt gccaaagttt aataattata ttaaagcaga gatgatggaa
     3481 ttagaagata ttgatgtatt aattaca
//
LOCUS       DALAGD010000161         3404 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.167, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000161 DALAGD010000000
VERSION     DALAGD010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.167"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            652..1029
                     /locus_tag="O2701_002671"
     CDS             652..1029
                     /locus_tag="O2701_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCZ4294529.1"
                     /translation="MNKKYLMIVIIILILILGGIFLKMKYDEKKYYDEQKERITIYMK
                     YNVKGYKNISFANFKENPMDGYSISGYINNDKKLSFTAGIRSVDDFQFDTDISYTDEL
                     GRKFNKNPKSVSEIKKEQNTSNK"
     gene            1223..1399
                     /locus_tag="O2701_002672"
                     /pseudo
     CDS             1223..1399
                     /locus_tag="O2701_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1377..1478)
                     /locus_tag="O2701_002673"
     CDS             complement(1377..1478)
                     /locus_tag="O2701_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294530.1"
                     /translation="MQGFKEKHQELKKALCQIGLMRSISEVKQLNIA"
     gene            complement(1601..1696)
                     /gene="pepA1"
                     /locus_tag="O2701_002674"
     CDS             complement(1601..1696)
                     /gene="pepA1"
                     /locus_tag="O2701_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin
                     PepA1"
                     /protein_id="HCZ4294531.1"
                     /translation="MMLIFVHIIAPVISGCAIAFFSYWLSRRNTK"
     gene            1899..2042
                     /locus_tag="O2701_002675"
     CDS             1899..2042
                     /locus_tag="O2701_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCZ4294532.1"
                     /translation="MTRERRSFSSEFKLQMVRLYKNGKPRNEIIREYDFTPSTFVNGG
                     YKM"
     gene            2646..3029
                     /locus_tag="O2701_002676"
     CDS             2646..3029
                     /locus_tag="O2701_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294533.1"
                     /translation="MSNQNLFDELEKKGYKLEDIFTKEEIKKYKAEDQLRAGKTQYVE
                     TGKDTATLYLSSAYTKTIAALGAGAISVISALTGGLVGAGVGGFLGSIAASNIDTSKG
                     IYIKLKTKKNAAGEYVLTGEKWGYQ"
     gene            3040..3216
                     /locus_tag="O2701_002677"
     CDS             3040..3216
                     /locus_tag="O2701_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294534.1"
                     /translation="MDRLKYSLKVGILALLLFCTLNYLVPMQSNAFSIIIYSAIFAVL
                     LMLLVYIFLGILKK"
     gene            3218..3403
                     /locus_tag="O2701_002678"
     CDS             3218..3403
                     /locus_tag="O2701_002678"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294535.1"
                     /translation="MKQISIGYEEIYGDRIFHSHFLKEHEVKNWKTVNQIIEKEHLDK
                     NEYEAEPPLFFFSGSLD"
BASE COUNT         1342 a          421 c          557 g         1084 t
ORIGIN      
        1 ggaaaatata tctagaaaaa atttctcttc gagatagaca agaatggtgg tacaatgttt
       61 tttatgacga tggcaaatat aataagaaaa taattaagaa tgacatgtca aaaattaggc
      121 gtaattttta gatttatcta tatatttatg ccaccgctca aacttttata aaaagtaaaa
      181 aaacaatatt tatattgaac ttgtgacgtg aatttctaaa acacaaccaa caatccacaa
      241 ggaaactagt aaataagttt aaataagatt tcgaaaaaat gtgtaatatt ataatatgaa
      301 aatctttaag attatagaag aatcatactg actaaaaaga ttttgaaagt gattaagtat
      361 ttaaaacacg ccaatcttaa accgctattg acaaatatga agcggaacca ccgttgtttt
      421 tcttttcagg tccaattgaa taaatgttat gaatagaatg gataaaaata taattattta
      481 agtgttaaag taaaccaaat catgatttaa gattataaga aagaaaaatt gactaatgtt
      541 aaaaccaaca atcatatggg ttgtaacgtt tatacaatca tcgtaaaact caatatgctt
      601 aatttattca aaatgttcgt caataagtac aatgaaaagg agattgattt tttgaataaa
      661 aaatatctta tgattgtaat tataatttta atattgattc taggcggaat ctttttgaag
      721 atgaaatatg acgaaaagaa atattatgat gaacaaaaag aaagaataac gatttatatg
      781 aagtacaatg tgaaaggtta taaaaatata agcttcgcta attttaaaga aaacccaatg
      841 gatggttatt ctattagtgg ttatataaat aatgataaaa agttatcatt tacagctggt
      901 ataagatctg ttgatgattt tcaatttgat accgatattt cttatacaga tgaattgggt
      961 agaaaattta ataaaaatcc taagtcagtt tctgaaataa aaaaagagca aaatacgtcc
     1021 aataaataat tgttcatatt gtgatgaaac aaaaatataa gtcattagat gagtttaact
     1081 atgttataaa tatttgtagt atctataaaa atctcgacac tattaaaatg ataaagtgcc
     1141 gaggttttct tacttattta gttaattcaa agtttatgcc agattcataa gaatttgtga
     1201 cacttttaat agtgtaacca ttgattatta caatttatca aatggtcctt tagaaggtat
     1261 aaataacaaa ataaaattaa ttaaacgtac atcttttggt tatggaagtt acaatcattt
     1321 gcgcaatcga atattattat gttcaaaact ttacgctcca aaaagtaaaa aggaagttaa
     1381 gcaatgttta gttgcttaac ttcggatatt gaacgcatca gtccaatttg acatagagcc
     1441 ttttttagtt cttgatgttt ctctttaaaa ccttgcatat tttacaaaaa gaaaaattag
     1501 cagtataatt aagacaacga aaataagtat ttacttatac accaatcccc tcactatttg
     1561 cggtagtgag gggattttta ttggtgcggc tatatgtcac ctattttgta ttgcgtctac
     1621 ttagccaata agaaaaaaac gcaatggcac agccactgat gactggtgct atgatgtgaa
     1681 cgaaaataag catcacctta tacacctcct ctctgcgtct aaattgacgc ctgagagata
     1741 ggcgactcta ctattatatc atcggcaaat atacaagcac agtcacttgc ttctgataag
     1801 ttatatgatt ctagctgata gattgaatcg tctacactta attggacaaa ttctatgaga
     1861 atagatattg ttaatttaag aaagtaggcg attttattat gacaagagaa agaagatcat
     1921 ttagttcaga gtttaagtta caaatggtta gattatataa aaatggtaag cctaggaatg
     1981 aaattatacg cgagtatgat ttcacacctt cgacgtttgt aaatggcggt tataaaatgt
     2041 aggaaaatgg ataaagcaac atcaaaacac gggtacattc aatcaccaag ataacttatc
     2101 ggatgaagaa aaagagctga ttaaattacg caaagaagtt caacatttaa aaatggagaa
     2161 cgatatttta aagcaagtag cgctgattat ggggcaaaaa tagaagtcat tcaaaagaat
     2221 gcacatcaat attcagtatc agcaatgtgt aaagtcctga taatactaag aagtacctat
     2281 tatgattcta taaaaagaaa agataataaa atcactaaag atgattcaaa catagaacat
     2341 gccgtcataa atatttttaa ttctaataga aaagtctttg gtacaagacg aattaaaaat
     2401 catttaaatg acaagggtct cactgtatct ggacaaaaga taggtcgatc atgaaaaaat
     2461 ctagtttctg tttatacgaa atctaaatac aaaaatcatc taaaagaaac taatgaaaaa
     2521 cgaattaaaa atctttatta ttagctgctg gtgtattatt tgttagtcca atttcattat
     2581 cttataattc agatgtagct catgctgaag ataagttaga ccattctcaa gcaaaggtaa
     2641 tatatttgag taaccaaaat ttatttgatg aacttgagaa aaaaggttat aaactggaag
     2701 atatatttac aaaagaagaa ataaaaaaat ataaagctga agaccaattg agagcgggta
     2761 aaactcaata tgtagaaaca ggtaaagata ctgcaacatt atatctttct tctgcatata
     2821 caaaaacaat agctgcttta ggtgcggggg ctattagcgt tatttctgca ctaacaggag
     2881 gtttagttgg cgcaggtgtt ggtggtttcc ttggatctat agctgcatcg aatattgata
     2941 ctagtaaggg aatatatata aaattaaaaa ctaaaaagaa tgcagccggg gaatacgttc
     3001 tgacaggaga aaaatgggga tatcagtaag ggtgatttta tggatagatt aaaatattca
     3061 cttaaagttg gaattttagc attattatta ttttgtactt taaattattt agttccaatg
     3121 caaagcaatg ctttttcaat aattatatat tcggcaattt ttgctgtgtt acttatgctt
     3181 ttagtttata tatttttagg aattttaaag aaatgacatg aaacaaatta gcattggcta
     3241 tgaagaaatc tatggggata gaatttttca tagccatttt ttaaaagagc atgaagtaaa
     3301 aaattggaaa accgtcaatc aaataattga aaaagaacac ttggacaaaa atgaatatga
     3361 agcggaaccg ccgttatttt tcttttcagg atcgttagat taga
//
LOCUS       DALAGD010000162         3346 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.168, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000162 DALAGD010000000
VERSION     DALAGD010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.168"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(265..969)
                     /locus_tag="O2701_002679"
     CDS             complement(265..969)
                     /locus_tag="O2701_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HCZ4294536.1"
                     /translation="MYNEILGLVTFIATFVLMVLMYRFFGKQGLIAWVAIGTIIANIQ
                     VIKTVEIFGISATLGNVMFASIYLATDILNDIYGRRVAKRAVWLGFSSTLIMIIVMQL
                     SLHFIPAPEDMAQKALHAIFDVVPRIALGSIVAYIIGQHIDVFIFSLIKKVFSSDKTF
                     FIRAYGSTFLSSIIDTALFVAIAFIGSLPGTVVFEIFITTYVLKLASTVFNVPFGYIA
                     KSFYRKGKIDKLDQGY"
     gene            1214..1408
                     /locus_tag="O2701_002680"
     CDS             1214..1408
                     /locus_tag="O2701_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HCZ4294537.1"
                     /translation="MSRILTFSEQNALAKIDDLMNTYCNQCPIKTRLRKLEGKTKAHH
                     FCINECSIGKEIKQLGNELQ"
     gene            1420..1671
                     /locus_tag="O2701_002681"
     CDS             1420..1671
                     /locus_tag="O2701_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HCZ4294538.1"
                     /translation="MKIISISETPNHNTMKITLSESREGMTSDTYTKVDDSQPAFIND
                     ILKVEGVKSIFHVMDFISVDKENDANWETVLPKVEAVFE"
     gene            1709..2833
                     /locus_tag="O2701_002682"
     CDS             1709..2833
                     /locus_tag="O2701_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor C"
                     /protein_id="HCZ4294539.1"
                     /translation="MEILRIEPTPSPNTMKVVLSYTREDKLSNTYKKVEETQPRFINQ
                     LLSIDGITSIFHVMNFLAVDKAPKADWEVILPDIKAAFSDANKVLESVNEPQIDNHFG
                     EIKAELLTFKGIPYQIKLTSADQELREQLPQTYVDHMTQAQTAHDNIVFMRKWLDLGN
                     RYGNIQEVMDGVLEEVLTTYPESQLPVLVKHALEENHATNNYHFYRHVSLDEYHATDN
                     WKTRLRMLNHFPKPTFEDIPLLDLALSDEKVPVRRQAIVLLGMIESKEILPYLYKGLR
                     DKSPVVRRTAGDCISDLGYPEALPEMVLLLDDPQKIVRWRAAMFIFDEGNAEQLPALK
                     AHINDNAFEVKLQIEMAISRIENGDEALGSVWKQMANRTI"
     gene            2849..3286
                     /locus_tag="O2701_002683"
     CDS             2849..3286
                     /locus_tag="O2701_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HCZ4294540.1"
                     /translation="MNAYDAYMKEIAQQMRGELTQNGFTSLETSEAVSEYMNQVNADD
                     TTFVVINSTCGCAAGLARPAAVAVATQNEHRPTNTVTVFAGQDKEATATMREFIQQAP
                     SSPSYALFKGQDLVYFMPREFIEGRDINDIAMDLKDAFDENCK"
BASE COUNT         1228 a          524 c          605 g          989 t
ORIGIN      
        1 aattctagtc aggggcccca acacagagaa tttcgaaaag aaattctaca ggcaatgcga
       61 gttggggtgt gggtcccaac acagaagatg acgaaaagtc agcttacaat aatgtgcaag
      121 tttgggatgg gccccaacaa agagaaattg gattcccaat ttctacagac aatgcaagtt
      181 ggggtgggac gacgaaataa attttgcgaa aatattattt ctgtcccact cccttaaaac
      241 ttattctttt gtgtagtaag tgcgttaata gccttgatct aacttatcaa tcttaccttt
      301 acgataaaat gatttagcaa tatatccaaa tggtacattg aaaactgttg aagctaattt
      361 taatacgtac gttgtaataa atatttcaaa tacaactgta ccaggtaaac ttccgataaa
      421 tgcgatagct acaaataaag ctgtatcaat tattgagctt aaaaatgtac taccatatgc
      481 acggatgaaa aacgttttat cagaactaaa tactttttta attagtgaaa agataaatac
      541 atcaatatgt tgaccaataa tatatgcgac gattgagcct aaagcaatgc gtggcacaac
      601 atcaaagatt gcgtgtaatg ctttttgtgc catatcttct ggtgcaggaa taaaatgtaa
      661 tgacaattgc ataacaataa tcataattaa tgttgatgaa aagcctaacc aaactgctct
      721 ttttgcaact ctacgcccat aaatatcgtt taatatatct gttgctaaat aaatagaagc
      781 aaacatgaca ttacctaaag ttgctgaaat accaaagatt tctacagttt taatcacttg
      841 tatgttggca atgattgtgc caattgcaac ccatgcaatt aaaccttgtt taccaaaaaa
      901 gcgatacata agtaccataa gcacgaacgt tgcaataaac gtaactagtc ctaaaatttc
      961 attatacata ttaaaatgtc ctcctaaatt ttgatcatgc gggtgtttag aaaccgctca
     1021 ataaataact aactcatttt actgaaaaat cgtttgcgat gcaatagcat ttgaatataa
     1081 atacatttta cgtatagaat tgtaaaaggt ttcattcaaa tcttagggtc aaaaatgtta
     1141 taatattttt atgtcaaatt taaaacagta acacttattt acaaggttgc aatattttga
     1201 agtaataaag gaagtgtcgc gtattttaac tttttcagag caaaatgcac tcgcgaaaat
     1261 agatgattta atgaatactt attgcaatca atgtccaatc aaaactcgtc tgcgtaaatt
     1321 agaggggaaa acgaaggcgc atcatttttg tatcaatgag tgttcaatag ggaaagaaat
     1381 aaaacaatta ggaaatgaac ttcaatagga ggaagtcaaa tgaaaattat atctatatca
     1441 gaaacaccga accacaacac aatgaagatt acacttagtg aaagcagaga aggtatgaca
     1501 tcagatacgt atactaaagt tgatgattca cagccagcat ttattaatga catcttaaag
     1561 gttgaaggcg ttaaatcaat tttccatgtt atggacttta tttcagtaga taaagaaaat
     1621 gacgcaaatt gggaaacagt attgccaaaa gtagaggctg tattcgaata aatttttcat
     1681 caactagtat tcggggggaa taaagtatat ggaaatttta cgtatagagc caacaccaag
     1741 tccaaataca atgaaagttg ttttgtcata tacaagagaa gacaagttat ctaatactta
     1801 taaaaaagta gaagaaacac aaccaagatt tataaatcag ttgttatcta tagatggtat
     1861 cacttccatt tttcatgtca tgaacttctt agctgttgat aaggcaccaa aagctgattg
     1921 ggaagtcata ttacctgata ttaaagctgc tttttctgat gcgaataagg ttttagaatc
     1981 tgtaaatgaa cctcaaattg acaatcattt tggtgaaatt aaagctgaat tattaacttt
     2041 taagggtata ccgtatcaaa ttaagctaac ttctgctgac caagaattaa gagaacaatt
     2101 accacaaaca tatgttgacc atatgactca agcgcaaaca gcacatgaca atattgtttt
     2161 tatgcgtaaa tggctagatt taggaaatcg ctatggaaat attcaagaag taatggatgg
     2221 tgtcctagaa gaagtgctaa ctacctatcc agaatcacag ttacccgtat tggtaaaaca
     2281 tgctttagaa gaaaatcacg caactaataa ttatcatttc tatcgacatg tctctttgga
     2341 tgaatatcat gcaactgata attggaagac tcgattacga atgttaaacc attttccaaa
     2401 gccgactttt gaagatatac cgctgcttga tttagcttta tctgatgaaa aagtaccggt
     2461 tagacgtcaa gcgattgtat tattaggtat gattgaaagt aaagaaattt taccgtattt
     2521 atataagggg cttcgtgata aaagtcctgt tgtaagaaga acagcagggg attgcataag
     2581 cgatttaggg tatccagagg cactaccaga aatggtgcta ctattagatg atccacagaa
     2641 aatcgttagg tggcgtgctg ctatgtttat ctttgatgaa ggtaatgcag agcagcttcc
     2701 cgcactaaaa gcccatatta atgacaatgc gtttgaagtt aaattacaaa ttgaaatggc
     2761 catatctcgt attgaaaacg gagatgaagc acttggttca gtatggaaac aaatggcaaa
     2821 cagaacaatt taattggagg aattaaatat gaatgcatat gatgcttata tgaaagaaat
     2881 tgcgcaacaa atgcgtggcg aattaactca aaatggtttt acaagtttag aaacgagcga
     2941 agcagtatcg gagtatatga accaagtaaa tgctgatgac actacttttg tagttattaa
     3001 ctctacatgc ggctgtgcag ctggattagc aagaccagct gcagtagcag ttgcaacaca
     3061 aaatgaacat agacctacaa atacagttac agtttttgct gggcaagata aagaagcaac
     3121 tgctacaatg cgagaattca ttcagcaagc accatctagt ccttcgtatg ctttattcaa
     3181 aggtcaagat ttagtttatt ttatgcctag agaatttatc gaaggtagag atattaatga
     3241 cattgcaatg gacttaaagg atgcctttga cgaaaattgt aaatagtaca cataaataaa
     3301 tataaaggtt aacacatttt ataatattaa aaatggtgtc tgtcat
//
LOCUS       DALAGD010000163         3320 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.169, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000163 DALAGD010000000
VERSION     DALAGD010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.169"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            208..933
                     /locus_tag="O2701_002684"
     CDS             208..933
                     /locus_tag="O2701_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL14"
                     /protein_id="HCZ4294541.1"
                     /translation="MKKNIMNKLVLSTALLLLETTSTQLPKTPISFSSEAKAYNISEN
                     ETNINELIKYYTQPHFSLSGKWLWQKPNGSIHATLQTWVWYSHIQVFGSESWGNINQL
                     RNKYVDIFGTKDEDTVEGYWTYDETFTGGVTPAATSSDKPYRLFLKYSDKQQTIIGGH
                     EFYKGNKPVLTLKELDFRIRQTLIKNKKLYNGEFNKGQIKITADGNNYTIDLSKKLKL
                     TDTNRYVKNPRNAEIEVILEKSN"
     gene            1028..1753
                     /locus_tag="O2701_002685"
     CDS             1028..1753
                     /locus_tag="O2701_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL13"
                     /protein_id="HCZ4294542.1"
                     /translation="MNNNITKKIILSTTLLLLGTASTQFPNTPINSSSEAKAYYINQN
                     ETNVNELTKYYSQKYLTFSNSTLWQKDNGTIHATLLQFSWYSHIQVYGPESWGNINQL
                     RNKSVDIFGIKDQETIDSFALSQETFTGGVTPAATSNDKHYKLNVTYKDKAETFTGGF
                     PVYEGNKPVLTLKELDFRIRQTLIKSKKLYNNSYNKGQIKITGADNNYTIDLSKRLPS
                     TDANRYVKKPQNAKIEVILEKSN"
     gene            1861..2577
                     /locus_tag="O2701_002686"
     CDS             1861..2577
                     /locus_tag="O2701_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL12"
                     /protein_id="HCZ4294543.1"
                     /translation="MSKNITKNIILTTTLLLLGTVLPQNQKPVFSFYSEAKAYSIGQD
                     ETNINELIKYYTQPHFSFSNKWLYQYDNGNIYVELKRYSWSAHISLWGAESWGNINQL
                     KDRYVDVFGLKDKDTDQLWWSYRETFTGGVTPAAKPSDKTYNLFVQYKDKLQTIIGAH
                     KIYQGNKPVLTLKEIDFRAREALIKNKILYNENRNKGKLKITGGGNNYTIDLSKRLHS
                     DLANVYVKNPNKITVDVLFD"
     gene            complement(3022..3246)
                     /locus_tag="O2701_002687"
     CDS             complement(3022..3246)
                     /locus_tag="O2701_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294544.1"
                     /translation="MLIQERGLKMTELNNIINSLQSLFESESGYKISKNSGVPYQTVQ
                     DLRNGKTKLEDARFRTIIKLYSYYVSLKEH"
     gene            complement(3203..>3320)
                     /locus_tag="O2701_002688"
     CDS             complement(3203..>3320)
                     /locus_tag="O2701_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294545.1"
                     /translation="IIWLNIFLGNEIIHTLTVLITTLYIVNSRKGIKNDRVE"
BASE COUNT         1241 a          506 c          482 g         1091 t
ORIGIN      
        1 aataattcta acgtatcgtt tttaatatat aactcccgcc tactttatta gatactgttt
       61 ctgtccaaaa cttaaaaatg ataagttttg cttaaataac actactaact gtttaagttt
      121 atttaacata gttttagctt ttatttaatt ccgaatcggt gtaatagctt atatactttg
      181 ggtaattcac gcaaaggaga ttttcatatg aaaaagaaca tcatgaataa attagtttta
      241 tcaacagcat tgttactttt agaaactaca tcaacacaac ttcctaaaac accaatcagt
      301 ttttcatctg aagcaaaagc ctataatatc agtgaaaacg agactaatat caatgaacta
      361 atcaaatatt acactcagcc gcatttttca ttatctggaa aatggttatg gcaaaagccc
      421 aatggtagca ttcatgcaac attgcaaacg tgggtttggt atagtcatat tcaagtgttt
      481 ggatccgaga gttggggaaa cattaatcag ttaagaaata aatacgttga tatatttgga
      541 actaaagatg aggacacagt tgaaggttac tggacttatg atgaaacatt tactggtggt
      601 gttacgccag cagctacttc atctgataag ccttatagac tatttttaaa atatagtgat
      661 aaacaacaaa ctatcatcgg tggacatgaa ttttacaaag gaaataaacc agtattaact
      721 ttaaaagaat tagatttccg tattcgtcaa acattaataa aaaataaaaa gttatataac
      781 ggagaattta ataaaggtca aattaagata actgctgatg gaaataatta cacgattgat
      841 ttaagtaaaa agttaaaatt aactgacaca aaccgttatg ttaaaaatcc tcgtaatgca
      901 gaaattgaag tcatactcga aaaatctaac taacctatta ccttttgtaa atgcggataa
      961 tttcaattat ctaattaacc cctttttata attaaacatt ccaacaatac tcaaaggaga
     1021 aattcgaatg aacaataaca tcacgaaaaa aattatttta tcaacaacat tgttactatt
     1081 aggtacagca tctacacaat ttcctaatac acctatcaat tcttcatctg aagcgaaagc
     1141 ttattatata aatcaaaacg aaactaacgt taatgagtta actaaatatt actcgcaaaa
     1201 atatttaacc ttctctaaca gtacgttatg gcaaaaagat aacggtacga ttcatgcaac
     1261 gttgttacag ttttcttggt atagtcatat tcaagtttat ggacctgaaa gttggggcaa
     1321 tatcaaccaa ttaagaaata aaagcgttga tatttttggc ataaaagacc aagaaaccat
     1381 tgattctttt gcattatctc aagaaacgtt tactggtggt gttactcctg cagcaacatc
     1441 taacgataaa cactataaac tgaatgtaac atataaagat aaagcagaaa cgtttactgg
     1501 cggatttcca gtttatgaag gcaataagcc tgttttaact ttaaaagaat tagattttcg
     1561 tattcgtcaa acattaatta aaagtaaaaa attatataat aattcttata ataaaggaca
     1621 aattaaaata acaggtgcag acaataacta cacaatagat ttaagtaaaa ggttgccatc
     1681 aactgatgca aatagatatg ttaaaaaacc tcaaaatgca aaaattgaag ttatcctcga
     1741 aaaatcaaac taacaataat aatggagtta ataaaaataa tcgcaaatac tatattgact
     1801 tcgctcacat ttaaatttct tattcctcgt atcatgattc ctctgaaagg agatgttcta
     1861 atgagtaaga acatcacgaa aaatataatt ttaacgacaa cattattact attaggtact
     1921 gtattacctc aaaatcaaaa accagtattt agtttttact ctgaagctaa agcttatagc
     1981 attggtcaag atgaaactaa catcaatgaa ttaattaaat attacacaca gcctcatttt
     2041 tcattttcaa ataaatggct atatcaatat gataatggaa acatttatgt tgaacttaag
     2101 agatattcat ggtcagcaca tatatcttta tggggcgctg aaagttgggg aaatattaat
     2161 cagttaaaag atcgttacgt agatgtgttt ggactaaaag acaaagatac tgatcagtta
     2221 tggtggtctt atagagagac atttacaggt ggcgttacac cagccgcaaa accttctgat
     2281 aaaacttata atctttttgt gcaatacaaa gataaactac aaacgattat tggtgcgcat
     2341 aaaatatacc aaggcaataa accagtatta acattgaaag aaatcgattt ccgtgcacga
     2401 gaagcgttaa taaaaaataa aatattatat aacgaaaatc gtaataaagg taagcttaag
     2461 atcaccggtg gcggtaataa ctacactatt gatttaagca aaagattaca ttcagatcta
     2521 gcaaatgttt atgttaaaaa tcctaataaa ataactgttg acgtcctctt tgattagtat
     2581 atgaaggtga cttatacttc atgcacttta attccaaatc agattattta aatgataatt
     2641 tttaaagtgt atgatgtata taataggtaa aattttctat atatttaaat ggaattggga
     2701 gtaggaatgt gacagaaata gtattttata aaatttattt cgttgtcact ccccaacttg
     2761 cacattattg taagctgact tttcgtcagc ttctgtgttg gggcccaccc caacttgcat
     2821 tgtctgtaga atttcttttt gaaattctct atgttggggc cccgcctata attgaaaaat
     2881 gcttgttaca tgggcatttt cattcggtca actactacca atataatatt gtagagccta
     2941 agacattgat ttattatgtc ttaggctcta ttccttcatt taatgattaa attattatag
     3001 caatacttta ttgtcccatg attagtgttc ttttaatgag acatagtaac tataaagttt
     3061 aataatcgtt ctaaatctag catcttctag tttggttttc ccatttctta aatcttgtac
     3121 tgtttgatat ggaactcctg aatttttcga aattttatag cctgattcag actcaaacaa
     3181 agattgaaga gagtttataa tattattcaa ctctgtcatt tttaatcccc tttcttgaat
     3241 taacaatata caatgttgtt attaaaacag tcagtgtatg gatgatttca tttcctaaaa
     3301 atatattcag ccatataata
//
LOCUS       DALAGD010000164         3312 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.170, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000164 DALAGD010000000
VERSION     DALAGD010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.170"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(<1..302)
                     /locus_tag="O2701_002689"
     CDS             complement(<1..302)
                     /locus_tag="O2701_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMEK domain-containing protein"
                     /protein_id="HCZ4294546.1"
                     /translation="MKRSTNQEKFLDTLIRLNTKIEELGKINILNNHIYSEYFFRDLL
                     NIVYGYSLENHNKKQKNAPAFDLIDNTNKIIIQVTATCKKQKIEDTLKKEYLTNKM"
     gene            complement(1152..2351)
                     /locus_tag="O2701_002690"
     CDS             complement(1152..2351)
                     /locus_tag="O2701_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCZ4294547.1"
                     /translation="MSNTQKKNVPELRFPGFEGEWEEKKLGNLTTKIGSGKTPKGGSE
                     NYTNKGIPFLRSQNIRNGKLNLNDLVYISKDIDDEMKNSRTYYGDVLLNITGASIGRT
                     AINSIVETHANLNQHVCIIRLKKEYYYNFFGQYLLSRKGKRKIFLAQSGGSREGLNFK
                     EIANLKIFTPTIFEEQQKIGKFFSKLDRQIELEEQKLELLQQQKKGYMQKIFTQELRF
                     KDENGEEYPEWENKFIKDIFIFENNRRKPITSSLREKGLYPYYGATGIIDYVKDYLFN
                     NEERLLIGEDGAKWGQFETSSFIANGQYWVNNHAHVVKSNDHNLFFMNYYLNFKELRA
                     FVTGNAPAKLTHANLCNINLKIPCLTEQDKVSALLKSIDNKMNNQMNRIELLKERKKG
                     LLQKMFI"
     gene            complement(2344..>3312)
                     /locus_tag="O2701_002691"
     CDS             complement(2344..>3312)
                     /locus_tag="O2701_002691"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCZ4294548.1"
                     /translation="GKKAGEFYTPQQVSKILAKIVTDGKDKLRHVYDPTCGSGSLLLR
                     VGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYENFDIRNDDTLENPAFLGNTFDAV
                     IANPPYSAKWTADSKFENDERFSGYGKLAPKSKADFAFIQHMVHYLDDEGTMAVVLPH
                     GVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYGTSIPTCILVFKKCRQQDDNVLF
                     IDASNDFEKGKNQNHLSDAQVERIIDTYKRKETIDKYSYSATLQEIADNDYNLNIPRY
                     VDTFEEEAPIDLDQVQQDLKNIDKEIAEIEQEINAYLKELGVLKDE"
BASE COUNT          952 a          568 c          450 g         1342 t
ORIGIN      
        1 tccattttat ttgttaaata ctcttttttt agagtgtcct ctattttttg ctttttacac
       61 gttgcggtaa cttgaataat aattttattc gtattatcta taagatcaaa tgctggtgca
      121 tttttttgtt ttttattatg attctcaagt gaatacccat atacaatatt taacaggtct
      181 ctaaaaaaat attcggaata tatatgatta tttaaaatat ttatctttcc tagttcttca
      241 atcttagtat taagtctaat tagtgtatca agaaattttt cttgattcgt tgatcgtttc
      301 aagttttaag tctcctttta aaatgaatat ttcacttctt ataactaata tttaaacaaa
      361 atgatcacat aataatattt aatttacaat atgccactac agtgtcaaca ttttataaac
      421 actattcatt tgaacatggt ctaaaacgaa taggacattg taaatgatta aatagtctgt
      481 ctctagttga tgtatatggt catttgttat gcagataata attaatctaa ttaattttta
      541 cgttttagtt acttggttgt gattgaggct ttttgtatct agttgtaata ttctcctttt
      601 ttagaatcat aataggtact tcttgaaatt tttagaactt tgcacattgc tgatactgaa
      661 tattgatacg cattttgttg aaagacttca ttttttgtct caaaattagc actgcttgct
      721 ttaaatcatc attttacatt atcaatcgat gtttttcttt gcgtaatttt attaactctt
      781 tttcatcact tgttaaaatg tcttgatgat tgaatgactc agtattttgg ttttgtttta
      841 tctaatttga gaaagttaat gatgttagat catattctcg tataatttcg tttctaagct
      901 taccattttc ataaagttta attattttaa tttaaattat ttactaaaag ctcttcagtc
      961 tcttgtcata ataaaatcgc ctaccctctt agtttaacaa tgtctattct catagaattt
     1021 gtccaactaa atgtagacga ttcactaaac ttctaaaaaa ataaacccca aattataata
     1081 gtctcaatat taatatatta caatttattc ataggatttt tatcatttca attaataggt
     1141 tgttatcagg gttaaataaa cattttttgt aatagtcctt ttttacgttc ttttaataac
     1201 tcaattctat tcatttgatt attcatttta ttgtctatag attttaacaa tgcacttact
     1261 ttatcttgtt cagtgagaca aggtatttta agatttatat tgcataagtt cgcatgagtt
     1321 aatttagctg gtgcattacc tgtgacaaat gctcgtagtt ctttaaaatt taaataataa
     1381 ttcataaaaa acaaattatg atcattactt ttaactacat gcgcatgatt atttacccag
     1441 tattgcccat tagcaataaa gctactcgtc tcaaactgcc cccattttgc accatcttct
     1501 cctattagta atcgttcttc attattgaat aaataatctt ttacgtaatc aataattcca
     1561 gttgcaccat agtaagggta taaccccttt tctcttaatg aagaagtaat tggttttctt
     1621 ctattatttt caaagataaa gatgtctttt atgaatttgt tttcccactc tggatattct
     1681 tcaccattct catctttgaa tcgcaattct tgtgtgaaga ttttctgcat atagcctttt
     1741 ttctgttgtt gaagcaattc aagtttttgt tcttctaatt caatttgtcg gtcgagtttg
     1801 ctgaagaact tgcctatttt ttgctgttct tcaaatatag ttggggtgaa gatttttaaa
     1861 ttagcaattt ctttgaagtt tagaccttct cgactacctc cactttgtgc aaggaaaatt
     1921 ttccttttac cttttcttga taatagatac tgtccaaaaa aattataata atactctttt
     1981 ttcaatctaa taatacatac atgttgattt aaattagcat gcgtttcaac tatcgaatta
     2041 atggctgttc tacctattga tgctcctgta atatttaaaa gaacatcacc atagtacgtt
     2101 ctactatttt tcatctcatc atctatatct ttactaatat aaactaagtc attaagattt
     2161 aatttaccat ttctaatatt ttgactcctt aaaaatggta tgcctttgtt tgtatagttt
     2221 tcacttccac ctttgggagt ctttccacta cctattttgg tagtaagatt ccctaacttc
     2281 ttctcttccc attcgccttc aaatcctggg aatctcaatt ctggcacatt tttcttttgt
     2341 gtattactca tctttcaaca ccccaagttc tttcaggtat gcattgattt cttgctcaat
     2401 ttctgcgatt tctttatcga tatttttcaa atcttgttgg acttgatcta aatcaatcgg
     2461 tgcttcttct tcgaatgtat cgacatatct cggtatattt aggttgtaat cgttatcggc
     2521 aatctcttgt agtgtcgcgc tgtagctata tttatcaatt gtttccttac gcttatatgt
     2581 gtctataata cgttcgactt gggcatcgct taaatgattt tgattttttc ctttttcaaa
     2641 atcattggat gcatcgataa atagtacgtt gtcgtcttgt tggcgacatt ttttaaatac
     2701 taaaatacat gttggaatac ttgtcccata gaaaatattc gctggcaaac caatcacagc
     2761 ttctaagtag ttcttttctt caattaaata acgacgaatg acaccttctg cagcacctcg
     2821 gaataataca ccatgtggga gtacaacggc catggtacct tcatcgtcta ggtaatgtac
     2881 catgtgttga ataaaggcaa agtctgcttt agacttaggc gcaagtttgc cgtaaccact
     2941 gaatcgttcg tcattttcaa actttgaatc tgcagtccat ttcgcactat acggtgggtt
     3001 cgcaataacc gcatcaaatg tattgcctaa aaaggctggg ttttccaatg tgtcatcatt
     3061 acggatatcg aagttctcat aacgcacatc atgtaataac atattcatgc gtgctaagtt
     3121 gtatgtagta ttgttacgtt cttgaccgaa ataacgatac acttgtgttt ctttaccaac
     3181 acgtaacaac agtgaacctg aaccacatgt tgggtcatac acgtgacgta atttatcttt
     3241 accgtctgtg acaatcttcg ccagtatctt agatacttgt tgtggtgtat agaactcgcc
     3301 tgctttttta cc
//
LOCUS       DALAGD010000165         3203 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.172, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000165 DALAGD010000000
VERSION     DALAGD010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.172"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            140..775
                     /locus_tag="O2701_002692"
                     /pseudo
     CDS             140..775
                     /locus_tag="O2701_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            772..1302
                     /gene="sat4"
                     /locus_tag="O2701_002693"
     CDS             772..1302
                     /gene="sat4"
                     /locus_tag="O2701_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HCZ4294549.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFEKAVFWYLRF"
     gene            1395..2189
                     /locus_tag="O2701_002694"
                     /note="aph(3')-IIIa"
     CDS             1395..2189
                     /locus_tag="O2701_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HCZ4294550.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            2465..3036
                     /locus_tag="O2701_002695"
                     /pseudo
     CDS             2465..3036
                     /locus_tag="O2701_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
BASE COUNT         1075 a          504 c          729 g          895 t
ORIGIN      
        1 cgaacactga catgatatat tagtgattag ctatattttt tactttgcaa cagaaccatc
       61 atttttatta aaatactctt ttgtaaaatc tttctgctct tcaatgtatt taatttcttt
      121 acctctaaat ctattatagc tatttgatga ttacaataaa attgatctta ccttattgcc
      181 cttggaagag ttagataatt acctaaaggg cgataaatta ataaaggttc taattgataa
      241 agattgtaga attaaaaggg acatagttcc gactgatata gattatcatg taagaaagcc
      301 aagcgcaagg gagtatgatg attgctgcaa tgaattttgg aatgtaacac cttatgttat
      361 taaaggattg tgccgtaagg aaattttatt tgctattgat cattttaatc agattgttcg
      421 ccatgagctg ctgagaatga tatcatggaa ggtcggcatc gaaacaggct ttaaatgaag
      481 tgtaggcaag aactataagt ttattgaaag gtatatatcc gaggatttgt gggagaaact
      541 tttgtccacc taccggatgg attcctatga aaacatatgg gaagcattat ttctatgcca
      601 tcaattgttc agggcggtat ccggtgaggt ggcggaaagg cttcattatg cctatccgga
      661 gtatgatagg aatataacaa aatataccag ggacatgtat aaaaaataca ctggtaaaac
      721 cggctgcctg gatagcacat atgccgctga tatagaagag aggcgggaac agtgattaca
      781 gaaatgaaag cagggcacct gaaagatatc gataaaccca gcgaaccatt tgaggtgata
      841 ggtaagatta taccgaggta tgaaaacgag aattggacct ttacagaatt actctatgaa
      901 gcgccatatt taaaaagcta ccaagacgaa gaggatgaag aggatgagga ggcagattgc
      961 cttgaatata ttgacaatac tgataagata atatatcttt actaccaaga cgataaatgc
     1021 gtcggaaaag ttaaactgcg aaaaaattgg aaccggtacg cttatataga agatatcgcc
     1081 gtatgtaagg atttcagggg gcaaggcata ggcagcgcgc ttatcaatat atctatagaa
     1141 tgggcaaagc ataaaaactt gcatggacta atgcttgaaa cccaggacaa taaccttata
     1201 gcttgtaaat tctatcataa ttgtggtttc aaaatcggct ccgtcgatac tatgttatac
     1261 gccaactttg aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa
     1321 ttggagttcg tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag
     1381 gaaggaaata ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa
     1441 aataccgctg cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg
     1501 gagaaaatga aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg
     1561 atgtggaacg ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg
     1621 tcctgcactt tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg
     1681 tcctttgctc ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg
     1741 cggagtgcat caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct
     1801 tagacagccg cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt
     1861 gcgaaaactg ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa
     1921 agacggaaaa gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca
     1981 tctttgtgaa agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg
     2041 acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac
     2101 agtatgtcga gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat
     2161 attatatttt actggatgaa ttgttttagt acctagattt agatgtctaa aaagctttaa
     2221 ctacaagctt tttagacatc taatcttttc tgaagtacat ccgcaactgt ccatactctg
     2281 atgttttata tcttttctaa aagttcgcta gatagagttc tatattagag atgtaaagag
     2341 ttttggtgaa gaggtttgtg agtatggctt ttataaccag ggtgcaatga gaagcactaa
     2401 cagcagctag cttaagaacg cataggatat actttttatg gaagtcacag aggaggaata
     2461 aaatttgaac aggattaaca gagtaacctc tattcttatt cagctgcaat caaaaaaata
     2521 attcctgcca aagaaattgc acagcgattt aatataagct taaggacagt ttacagagat
     2581 atccggacac ttgaagaagc cggaatacca attggatctg aggccggaaa aggatatttt
     2641 cttgtagagg gcttcctact tctgccggta atgtttacag cggcggaagt gggtgcattg
     2701 attacagcag ggaaattttt aaattgccat ggagatgaat catttataaa ggattttgat
     2761 tcagctatgt ataaaatcaa atctattttg aagcatggtg aaaaaaacta tgcacaggag
     2821 ttggagaaca gtattaacgt gtacagcaca tctggacaga aaaatacatt ggcggataac
     2881 gttattgcag caattcagac tgcaatctgc aataaaaggg taatatcaat tcaatatcct
     2941 gcatcaggag ggcaagaacc ggaaagcaga atgatagagc ctatatcact ggggttttat
     3001 gaacagaact ggtacttaat cggttttgca ggctaagaaa tgaatatagg aattttcgtg
     3061 tggatcggat taaaagcatt tgcatagaag aagagctctg tcaaagccat gatggctctt
     3121 tggaacaaat attaaaacaa atgctggttc tgttgcaaag taaaaaaata tagctaacca
     3181 ctaatttatc atgtcagtgt tcg
//
LOCUS       DALAGD010000166         3182 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.173, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000166 DALAGD010000000
VERSION     DALAGD010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.173"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            56..628
                     /locus_tag="O2701_002696"
     CDS             56..628
                     /locus_tag="O2701_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenyl diphosphate synthase component 1"
                     /protein_id="HCZ4294551.1"
                     /translation="METTVSKLERQIEERLKGVSEYESININHRLGKLLDLYDIPDVA
                     KVACLTIDTSMRHLDDITYNHLSKHSILIGDLISAHFYTLLAEINDLSFQNEISKAIV
                     EINELKSSLHHQALNDYEISQAIVKIETLFPYITLSHFGINIDESEIYNYLFEDMSDY
                     YPSYFKKYNQSEVKHYLHDIQKSYLKSRGN"
     gene            631..1356
                     /locus_tag="O2701_002697"
     CDS             631..1356
                     /locus_tag="O2701_002697"
                     /EC_number="2.1.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethylmenaquinone methyltransferase"
                     /protein_id="HCZ4294552.1"
                     /translation="MADNKANKEQVHRVFQNISKKYDRLNNIISFEQHKVWRKRVMKD
                     MGVRKGTKALDVCCGTGDWTIALSKAVGPTGEVTGIDFSENMLEVGKEKTASMENVKL
                     VHGDAMELPFEDNSFDYVTIGFGLRNVPDYLVALKEMNRVLKPGGMVVCLETSQPTLP
                     VFKQMYALYFKFVMPIFGKLFAKSKEEYEWLQQSTFNFPGKEELKRMFEEAGFINVRV
                     RSFTGGVAAMHLGYKEKDNTKGD"
     gene            1358..2317
                     /locus_tag="O2701_002698"
     CDS             1358..2317
                     /locus_tag="O2701_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCZ4294553.1"
                     /translation="MAKLNMNNEIKKVEQRLEKAIKSKDSVLEQASLHLLSSGGKRVR
                     PAFVILSSQFGKDEQTSEQTYQVAVALELIHMATLVHDDVIDKSDKRRGKLTISKKWD
                     QTTAILTGNFLLALGLEHLMAVKDNRVHQLISESIVDVCRGELFQFQDQFNSQQTIIN
                     YLRRINRKTALLIQISTEVGAITSQSDKETVRKLKMIGHYIGMSFQIIDDVLDFTSTE
                     KKLGKPVGSDLLNGHITLPILLEMRKNPDFKLKIEQLRRDSERKEFEECIQIIRKSDS
                     IDEAKAVSSKYLSKALNLISELPDGHPKSLLLSLTKKMGSRNT"
     gene            2409..2858
                     /gene="ndk"
                     /locus_tag="O2701_002699"
     CDS             2409..2858
                     /gene="ndk"
                     /locus_tag="O2701_002699"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCZ4294554.1"
                     /translation="MERTFLMIKPDAVQRNLIGEVISRIERKGLKLVGGKLMQVPMEL
                     AETHYGEHQGKPFYNDLISFITSAPVFAMVVEGEDAVNVSRHIIGSTNPSEASPGSIR
                     GDLGLTVGRNIIHGSDSLESAEREINLWFNENEITSYASPRDAWLYE"
BASE COUNT         1177 a          417 c          596 g          992 t
ORIGIN      
        1 taatttctta catataatgt taatatgaat agtgataata tcgtgaggtg tagacatgga
       61 aacaactgtt agcaaattgg aaagacaaat agaagaaaga ttaaagggcg tatcagaata
      121 tgaatccatt aatattaatc atcggttagg taagttactt gatttatatg atattccaga
      181 tgttgccaaa gtggcatgcc tcacaattga cacatcaatg agacaccttg atgacattac
      241 atataatcat ttgtctaaac attccatttt gattggagat ttaattagtg cacattttta
      301 tacgctacta gcagaaatca atgatttatc atttcaaaat gaaattagta aagcaattgt
      361 tgaaattaat gaattgaaat cttcattaca tcatcaagct ttaaatgatt atgaaatttc
      421 tcaagcaata gtaaaaattg aaacactttt cccatatatt acgttatctc atttcggtat
      481 caatattgat gagtcggaaa tatataatta tttatttgaa gatatgtctg attactatcc
      541 atcatacttc aagaaatata atcaaagtga agtaaagcat tacttacacg atatccaaaa
      601 aagttattta aaaagtagag gtaattaagc atggctgaca ataaagcaaa taaagagcaa
      661 gtacatcgcg tttttcaaaa tatttcaaaa aaatatgata gattaaataa tattattagt
      721 tttgagcagc ataaagtatg gagaaaacgc gtcatgaaag acatgggtgt tagaaaaggg
      781 acgaaagcat tagatgtttg ttgtggtact ggtgattgga caatcgcatt aagcaaagct
      841 gtaggaccaa caggtgaagt tactggtatt gactttagtg agaatatgtt agaagtagga
      901 aaagaaaaaa ctgcttcaat ggaaaatgtt aaacttgttc atggtgatgc aatggaattg
      961 ccgtttgaag acaattcttt tgattatgta acaattgggt ttggattaag aaatgtgcca
     1021 gactatttag tcgcgttaaa agaaatgaat agagtactta aaccaggtgg tatggtggta
     1081 tgtcttgaaa cgagccaacc aactttgcca gtctttaaac aaatgtatgc actatacttt
     1141 aaatttgtta tgcctatttt tggaaaatta tttgcaaaat caaaagaaga atatgaatgg
     1201 ttacagcaat ctacgtttaa ttttcctgga aaagaagagt tgaagcgcat gtttgaagaa
     1261 gcaggtttca taaatgtaag agtacgtagt tttacagggg gcgttgctgc aatgcacctt
     1321 ggctataaag aaaaagataa taccaaaggt gattaacgtg gcaaagttaa acatgaacaa
     1381 tgaaattaag aaagtggaac aacgacttga aaaggcaata aaaagtaaag attctgtatt
     1441 agaacaggca tcattacact tattgtcttc tggtggtaaa agagtacgcc cagcatttgt
     1501 tattctgagt agccaatttg gcaaagatga acaaacgtct gaacaaacgt atcaagttgc
     1561 agtcgcatta gagttaattc atatggcaac acttgttcat gatgacgtta ttgataaaag
     1621 cgacaagcgt cgaggcaagt taaccatatc aaagaaatgg gatcagacaa ctgctatttt
     1681 aactgggaat tttttattgg cattaggact tgaacactta atggccgtta aagataatcg
     1741 tgtacatcaa ttgatatctg aatctatcgt tgatgtttgt agaggggaac ttttccaatt
     1801 tcaagaccaa tttaacagtc aacagacaat tattaattat ttacgacgta tcaatcgcaa
     1861 aacagcactg ttaattcaaa tatcaactga agttggtgca attacttctc aatctgataa
     1921 agagactgta cgaaaattga aaatgattgg tcattatata ggtatgagct tccaaatcat
     1981 tgatgatgta ttagacttca caagtaccga aaagaaatta ggtaagccgg tcggaagtga
     2041 tttgcttaat ggtcatatta cgttaccgat tttattagaa atgcgtaaaa atccagactt
     2101 caaattgaaa atcgaacagt tacgtcgtga tagtgaacgc aaagaatttg aagaatgtat
     2161 ccaaatcatt agaaaatctg acagcatcga tgaggctaag gcagtaagtt cgaagtattt
     2221 aagtaaagct ttgaatttga tttctgagtt accagatgga catccgaaat cactactttt
     2281 aagtttgacg aaaaaaatgg gttcaagaaa cacgtagtat ttatgaaaag tattgaaagc
     2341 gctttaccaa cctgttaata tataatagta atatacataa acctattaaa cacaggggga
     2401 tacataaagt ggaacgtaca tttttaatga ttaaaccaga tgcagtacaa agaaatctaa
     2461 ttggtgaagt aatttcaaga attgaaagaa aaggactaaa acttgtcggt ggtaaattaa
     2521 tgcaagtacc aatggaactt gctgaaacac attatggtga acaccaaggt aaaccatttt
     2581 ataatgattt aatttcattt attacatcag caccagtgtt cgcaatggta gttgaaggtg
     2641 aagatgcagt taatgtatct agacatatta ttggcagcac caatccttca gaagcttcac
     2701 caggatcaat tagaggtgat ttaggtttaa ctgttggtag aaatatcatt cacggttcag
     2761 attcattaga gtctgctgaa cgtgaaatta atctatggtt taatgaaaat gaaattacta
     2821 gctatgcttc accacgtgat gcatggttat atgaataaaa tataaactgt aaacctttac
     2881 gatttattta taaaggtaga aagggttttg ttatgtggtt agtcattatg attatacata
     2941 acaaggcccg ttttttatgt tgtagtaaat tacttgaaaa attttatagt tttttggtaa
     3001 cacgtattaa aaagagagga atattcttta tcaaatgaaa ctaaacagag agaaggggtt
     3061 gttaaaatga agaatattat ttcgattatt ttggggattt taatgttctt aaaattaatg
     3121 gaattactat atggtgctat atttttagat aaaccactta atcctataac aaaaattatt
     3181 tt
//
LOCUS       DALAGD010000167         3172 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.174, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000167 DALAGD010000000
VERSION     DALAGD010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.174"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(40..732)
                     /locus_tag="O2701_002700"
     CDS             complement(40..732)
                     /locus_tag="O2701_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HCZ4294555.1"
                     /translation="MNKAERQNLIITAIQQNKKMTALELAKYCNVSKRTILRDIDDLE
                     NQGVKIYAHYGKNGGYQIQQAQSKIALNLSETQLSALFLVLNESQSYSTLPYKSEINA
                     IIKQCLSLPQTRLRKLLKRMDFYIKFDDTQHMTLPMLFSDILIYCTERNVMLVDHRVD
                     DNIKAENVIFIGLLCKHGHWHAVIYDIAQDKTAELEIENIIDISYSFGKTIQTRDISI
                     DNYHQFLNPIDS"
     gene            1117..1914
                     /locus_tag="O2701_002701"
     CDS             1117..1914
                     /locus_tag="O2701_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HCZ4294556.1"
                     /translation="MTDKTLQQIDKLICSWLKQIDNVIPQLIMEMTTETKRHRFDLVT
                     NVDKQIQQQFQQFLATHFPEHQLLAEEKSNEMITNEINHLWIMDPIDGTANLVKQQED
                     YCIILAYFYEGKPMLSYVYDYPHKKLYKAIRGEGAFCNGIKMEEPPSLKLEDAIISFN
                     AQVMNLDTVQDLFDASFSYRLVGACGLDSMRVAKGQFGAHINTNPKPWDIAAQFLFAE
                     LLNLKMTTLDGKAIDHLKGAPFIISNKACHETVLKILNANGGYQKYR"
     gene            2047..2994
                     /locus_tag="O2701_002702"
     CDS             2047..2994
                     /locus_tag="O2701_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCZ4294557.1"
                     /translation="MKRSSKSKMSLPKKIFLWVFGILVILAIVAVVYVAAKIFITGNK
                     IHNPLDRNHSELRDKKVSLNDGDPFTIALFGVDSDADRKKKGGGERSDSIMILSINPK
                     TKKTEIVSIPRDTRAEIVGRGTTEKIAHAYAYGGPNMAVKSLEKLMNVPIDHYATIDM
                     DGLHNMIDSIGGVDVVSNDTFTVDGVRFTKGQQTHVNGDQALKFIRSRKEEGAGGDFG
                     RQQRQQIVLEAMANKIASPSSITHFNSLMNEIQNNVKTDLTLGDLNTIRSNYKDANDT
                     INKHQLSGQGGIQSDGLYYFIPSEQSKAESTKLLKDNLE"
BASE COUNT         1132 a          420 c          587 g         1033 t
ORIGIN      
        1 aagatatttt ctaattgaaa atcatcttac tgctgttttt taggaatcga tggggtttaa
       61 aaattgatga tagttatcaa tggatatgtc tctggtttga atcgtcttac cgaatgaata
      121 cgaaatatct ataatatttt caatttcgag ttcggcagtt ttgtcttgag caatgtcata
      181 aatgactgca tgccaatgtc catgtttaca caaaaggcca ataaatataa cgttttcagc
      241 tttaatatta tcatcaaccc tatgatctac taacatcaca tttcgttctg tacaataaat
      301 taaaatgtcg gaaaacagca ttgggagtgt catatgttgt gtgtcatcaa atttaatata
      361 aaagtccatg cgtttaagca attttcttaa gcgtgtttgt ggaagactta aacattgttt
      421 tataattgcg ttgatttcgc ttttatatgg taatgtcgag tacgactgac tttcattaag
      481 cactaaaaat aaggctgata attgtgtttc agataagttt aatgcaattt tagattgtgc
      541 ttgttgtatt tggtaaccac catttttccc ataatgcgca taaattttaa caccttgatt
      601 ttctaaatca tcaatatctc ttaaaattgt gcgtttggat acgttgcaat atttagctaa
      661 ttctaaagcg gtcatttttt tattttgttg aattgcagta attattaaat tttgcctttc
      721 agctttattc atgacagcca cctcctaaaa gatgatatat tacacaaagt ataacataag
      781 agtgacaatg aggacatttt caatatcttt taaaaaaaat cgtaaaaatt ttgtggaaaa
      841 cgcttacatt ttaaaaaaga ttgtgctatg atattacttg taacaggggg agcgattaaa
      901 caaaggggta gagctatata acagatagct tttaatcgtt caagttacat cacaacatct
      961 tcaatattta ttacttactt tcctttctat ttgtcggcta gcacgtgact agccgatttt
     1021 tttatgcata aaatgaagaa gtcttcagtt gtattgttga ttcttttaat atagggtaat
     1081 aataaaatga taatgatatg agaagtggtg gacgaaatga cagataaaac attacagcaa
     1141 atagataagt taatatgttc gtggttgaaa caaatagaca atgttattcc gcaattaatt
     1201 atggaaatga ctacagaaac taagcgtcat cgatttgatt tagttacaaa tgtagataaa
     1261 cagattcaac aacaattcca acaattttta gcaacacatt ttccggaaca ccaactatta
     1321 gcggaagaaa aaagtaatga aatgattacg aatgaaatta atcatttatg gattatggat
     1381 cccattgatg gaactgccaa tttagtaaaa cagcaagaag attattgtat tatattggcg
     1441 tatttttatg aaggtaaacc aatgttatcg tacgtatatg attatccgca taaaaagctt
     1501 tataaagcaa tacgaggaga aggtgctttt tgtaatggaa ttaagatgga agaaccacca
     1561 tcgttaaaat tggaagacgc tattatatcg tttaatgcac aagtgatgaa tctagatacg
     1621 gtgcaagatt tatttgacgc ctcatttagt tatcgtttag ttggtgcttg cggattagat
     1681 tctatgcgag tggcaaaagg tcaatttggg gcacatatta atacaaatcc taaaccttgg
     1741 gacattgctg cacagttttt atttgcagaa ctattgaatc taaaaatgac gactttagat
     1801 ggtaaggcaa ttgatcattt aaagggtgca ccttttatta taagtaataa agcatgtcat
     1861 gaaacggtac ttaaaatttt aaatgcaaat ggtggttatc aaaaatacag atagatattc
     1921 tctagagata aaaaatgata aaaaatagtt atacgtctac agaagtatag taaaaaaatt
     1981 attttactat ataataaata gagaaaacag acaaagtatg taaggaatag taaaggagtg
     2041 gtgggattga aacgttcgtc taaaagtaaa atgagcctac cgaaaaaaat atttttatgg
     2101 gtattcggta ttttagtaat cttagcgatt gtagctgttg tgtatgtagc tgctaaaatt
     2161 tttattactg gtaataagat acacaatccg ttagatcgca atcattcaga attaagagat
     2221 aaaaaagtga gtttaaacga tggtgatcca ttcacaatcg ctttatttgg tgtcgattca
     2281 gatgctgatc gtaagaaaaa gggtggcgga gagcgtagtg atagtattat gattttatct
     2341 atcaacccta aaacgaagaa aactgaaata gtaagtatac cgcgtgatac aagagcggaa
     2401 attgttggcc gtggtactac tgaaaaaatt gcacatgcct atgcatatgg tggaccaaat
     2461 atggctgtta aatcacttga aaaattaatg aatgtgccaa ttgatcatta tgcgacaatc
     2521 gatatggatg gtttgcataa tatgatagat agcatcggcg gtgttgatgt agtaagtaat
     2581 gatacattca ctgttgatgg tgttcgtttt acaaaaggac agcagacaca tgtaaatggt
     2641 gatcaagcat tgaagtttat tagaagtcgt aaagaagaag gtgctggtgg tgactttggt
     2701 cgacaacaac gtcagcaaat tgtgcttgaa gcgatggcca ataagattgc aagcccatca
     2761 tcgattacac attttaatag tctaatgaat gaaattcaaa ataatgttaa aacagattta
     2821 acattaggtg atttgaatac gattagaagc aattataaag atgctaatga cacgattaat
     2881 aaacatcaat tgagtggtca aggcggcatt caaagtgacg gattgtacta cttcattcct
     2941 agtgagcagt caaaagctga aagtactaag ttattaaaag ataatctaga gtagtaaact
     3001 aagggaaaat aagttatcaa aattgtacac aaattaatta gatagaatga tctgtaaata
     3061 gaataacgaa tagaactaat agcccgtaaa tagaaggtga taacactttg tatttatggg
     3121 ctattttatt taatgcctga tttgattttt ataaattaat ccattacaaa tt
//
LOCUS       DALAGD010000168         3130 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.176, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000168 DALAGD010000000
VERSION     DALAGD010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.176"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..546
                     /locus_tag="O2701_002703"
     CDS             <1..546
                     /locus_tag="O2701_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HCZ4294558.1"
                     /translation="KITTIIELIIIVISLLQWIRFIDFEKESAQKYKKIYARFLVVIN
                     VLTTITVVFALCNLYYFAAVQNHYDLFNYWLMGSISIIISYLLLVIGGMFTLLKLPKV
                     TKRWGGKTKTHFGLLLTALSSFIYIEKIIEYILVPNVVESKFIIIVSMLVIAGAQFVA
                     FQFIMQYSRFYIFELNTEDDD"
     gene            693..1304
                     /locus_tag="O2701_002704"
     CDS             693..1304
                     /locus_tag="O2701_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 family protein"
                     /protein_id="HCZ4294559.1"
                     /translation="MEFLLLIVVAGLYYIIYLTAVMYSEKIVVLPIIIYAIVFVIIGI
                     TYIFIGDSYDQLTNFNVILYMGSLFYAWMAIRNLWNRPLLLKYKNITDSSSGIVNKSE
                     YNSVESLRINIEIAKYKGIISLIVAIVLTVLMTLKSTPQITAETRDLSISFFILSLFI
                     IIIFAVWDLFIRVRKGAFAFVVIRPILFSCWLFILNMILSRLL"
     gene            1440..1658
                     /locus_tag="O2701_002705"
     CDS             1440..1658
                     /locus_tag="O2701_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294560.1"
                     /translation="MENQKQGNGLKIATWVFIVLTVVTPLFGIGSIVCSINYKKYDAE
                     KGSKLLQIAIIVTIIAFVLNLLAYLGLR"
     gene            1790..2290
                     /locus_tag="O2701_002706"
     CDS             1790..2290
                     /locus_tag="O2701_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCZ4294561.1"
                     /translation="MTFEEKLSEMYSEIANKISSMIPVEWEKVYAMAYIDDGGGEVFY
                     YYTEPGSNELYYYTSVLNKYDISESEFMDSVYELYKQFQNLRNLFKEEGHEPWTSCEF
                     DFTREGELKVSFDYIDWINSEFGQVGRQNYYKYRKFGILPETEYEINKVKEIEQYVKE
                     QEEAEQ"
     gene            2301..2801
                     /locus_tag="O2701_002707"
     CDS             2301..2801
                     /locus_tag="O2701_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCZ4294562.1"
                     /translation="MNFEEKLSEMYNKIANEINEMIPVEWEKVYVIAYVDDGGGQVIF
                     YYTKPRNDELYYYSSIVEDYNVLEEIFDDLWMELYRSFKKLRNIFKEESLEPWTSCEF
                     DFTKEDKLKVSFDYIDWKNTEFDQLGQENYYNYRKFGIIPEMEYEMEEVKQIEQYIKE
                     QEEAEQ"
     gene            2812..3130
                     /locus_tag="O2701_002708"
                     /pseudo
     CDS             2812..3130
                     /locus_tag="O2701_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498862.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
BASE COUNT         1190 a          301 c          577 g         1062 t
ORIGIN      
        1 aagataacga caattataga attaataatt attgtaatta gtttacttca atggattaga
       61 tttatagatt tcgaaaaaga aagtgcacag aaatataaaa aaatatatgc tcgattttta
      121 gttgttataa atgtgttaac tactatcact gtagtatttg cactgtgtaa tctttattat
      181 tttgcagctg tacaaaacca ttatgattta tttaattatt ggttgatggg ttcgatttca
      241 atcataatta gctatttatt attagtaatt ggcggaatgt tcacgttatt aaaattacct
      301 aaagtaacaa aacgttgggg tggtaaaact aaaacacatt tcggtttatt attaactgcg
      361 ttgagctcat ttatatatat tgaaaaaatt atcgaatata tattggttcc taatgtcgta
      421 gaatctaagt ttataattat tgtgagcatg ttggttatcg ctggagcaca atttgtggca
      481 tttcaattta ttatgcaata cagtagattc tatatttttg aattaaacac tgaagatgat
      541 gactaaacaa ttttataaat tctgtgaagt atacttatat ataagataat caacttcaac
      601 gtatatcacc atattatttt atactcaatg atgtgaatgg atttaagtgg attgatatta
      661 aagtaaaagt tatggaatat tgaggtgtga atatggagtt cttattatta attgtcgtag
      721 ccggactgta ttatattata tatttaactg ctgtgatgta ttctgaaaaa atagtagtat
      781 tgcctataat catctatgcc attgtgtttg taataattgg tatcacttat atctttatag
      841 gcgacagcta tgatcaatta acaaatttca atgtgatttt gtatatgggg agtttgtttt
      901 atgcatggat ggctattaga aatctttgga acagaccact attactaaaa tataagaaca
      961 ttacagatag ttcaagtgga atagttaata aatctgaata caattcagtt gaaagcttac
     1021 gtataaatat tgaaatagct aagtataaag ggattatttc tttgatagta gctatagtac
     1081 taacggtatt aatgacatta aagtcaacac ctcaaatcac tgcagaaaca cgtgacttaa
     1141 gtatctcatt tttcatactc agcttattta tcattattat atttgcagtt tgggatttgt
     1201 ttattagagt tagaaaagga gcgtttgctt ttgttgtaat aaggccaata ttatttagtt
     1261 gttggttatt tattttgaat atgattttat caagattatt ataattgtat tacatatagg
     1321 tgtgggtaaa tttttattta tgaaatgata aaaaagtata tactattaat atctttttta
     1381 ctataattat aaatagttaa ataaattgaa agacaaaata atgagtagga gttaatgaga
     1441 tggaaaacca aaaacaaggc aatggcttaa aaattgcaac atgggtattt attgtattaa
     1501 cagtagttac accgctattt ggtattggaa gtattgtttg tagtattaat tataaaaaat
     1561 acgatgctga aaaaggttcg aagttattgc aaattgcaat tatcgtaaca ataattgctt
     1621 ttgttttaaa tttattagca tatttaggtt taagataagt aaaacgaatt tgaagaagca
     1681 ttaagcgaca tttgggtgtt gtttaatgct ttttatttaa cggaaataag tgtagagaat
     1741 aaattaatag tttaatcaag agatatttcg aacacatagg ggcgataaca tgactttcga
     1801 agaaaaatta agtgaaatgt atagcgagat tgcgaataag attagcagca tgataccggt
     1861 agagtgggaa aaggtatatg caatggcata tatagatgac ggaggaggag aagtgttcta
     1921 ctattacaca gaacctggaa gtaatgaatt atactactat actagtgtat taaataaata
     1981 tgatatatcg gaatcggaat ttatggattc agtgtatgag ttgtataaac aatttcaaaa
     2041 tttaaggaat ttatttaaag aagaaggaca tgaaccatgg acatcatgcg aatttgactt
     2101 tacaagagaa ggtgaattaa aagtttcatt tgattatatt gattggataa attcagaatt
     2161 tggtcaagta ggtcgacaaa attactataa gtatagaaaa tttggaattt taccagaaac
     2221 ggaatatgaa attaataaag ttaaagaaat cgagcaatat gttaaagagc aagaagaagc
     2281 tgaacaatag aggcgataac atgaatttcg aagaaaaatt aagtgaaatg tacaataaaa
     2341 ttgcaaatga gatcaatgaa atgataccag tagagtggga aaaggtatat gtaattgcat
     2401 atgtggacga tggaggtggg caagttatat tttattatac caagcccaga aatgatgagc
     2461 tgtattatta ttcgagtatc gttgaagatt ataatgtttt agaagaaatc tttgatgatt
     2521 tatggatgga actttataga tcatttaaaa aattaagaaa tatatttaaa gaagaaagtc
     2581 ttgaaccatg gacatcatgt gaatttgact ttacaaaaga agataaatta aaagtttcat
     2641 ttgattatat tgattggaag aatacagaat ttgatcaatt aggccaagaa aactactata
     2701 actatagaaa attcggaatt ataccagaaa tggaatatga aatggaagag gttaaacaaa
     2761 tcgagcaata tattaaagag caagaagaag ctgaacaata gaggcgataa catgattttc
     2821 gaagaaaaac taaatgaaat gtacaacgag attgcgaata aaattagtag catgatacca
     2881 gtagaatggg aaaaggtata tacaatggct tatatagatg atggaggagg tgaagtattc
     2941 tttaattata ctaaacagcg atgaattgaa ttattacacc gatataccta aggagtataa
     3001 catttctgtg caagtatttg atgatttatg gatggattta tatgatttgt ttgaggaatt
     3061 aagaaattta tttaaagaag aaggactaga accatggaca tcatgcgaat ttgattttac
     3121 aagagaaggt
//
LOCUS       DALAGD010000169         3077 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.177, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000169 DALAGD010000000
VERSION     DALAGD010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.177"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            443..1378
                     /gene="lukE"
                     /locus_tag="O2701_002709"
     CDS             443..1378
                     /gene="lukE"
                     /locus_tag="O2701_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_114304764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukED subunit E"
                     /protein_id="HCZ4294563.1"
                     /translation="MFKKKMLAATLSVGLIAPLASPIQESRANTNIENIGDGAEVIKR
                     TEDVSSKKWGVTQNVQFDFVKDKKYNKDALIVKMQGFINSRTSFSDVKGSGYELTKRM
                     IWPFQYNIGLTTKDPNVSLINYLPKNKIETTDVGQTLGYNIGGNFQSAPSIGGNGSFN
                     YSKTISYTQKSYVSEVDKQNSKSVKWGVKANEFVTPDGKKSAHDRYLFVQSPNGPTGS
                     AREYFAPDNQLPPLVQSGFNPSFITTLSHEKGSSDTSEFEISYGRNLDITYATLFPRT
                     GIYAERKHNAFVNRNFVVRYEVNWKTHEIKVKGHN"
     gene            1380..2363
                     /gene="lukD"
                     /locus_tag="O2701_002710"
     CDS             1380..2363
                     /gene="lukD"
                     /locus_tag="O2701_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukED subunit D"
                     /protein_id="HCZ4294564.1"
                     /translation="MKMKKLVKSSVASSIALLLLSNTVDAAQHITPVSEKKVDDKITL
                     YKTTATSDNDKLNISQILTFNFIKDKSYDKDTLVLKAAGNINSGYKKPNPKDYNYSQF
                     YWGGKYNVSVSSESNDAVNVVDYAPKNQNEEFQVQQTLGYSYGGDINISNGLSGGLNG
                     SKSFSETINYKQESYRTTIDRKTNHKSIGWGVEAHKIMNNGWGPYGRDSYDPTYGNEL
                     FLGGRQSSSNAGQNFLPTHQMPLLARGNFNPEFISVLSHKQNDTKKSKIKVTYQREMD
                     RYTNQWNRLHWVGNNYKNQNTVTFTSTYEVDWQNHTVKLIGTDSKETNPGV"
     gene            2605..2748
                     /locus_tag="O2701_002711"
     CDS             2605..2748
                     /locus_tag="O2701_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gallidermin/nisin family lantibiotic"
                     /protein_id="HCZ4294565.1"
                     /translation="MEKVLDLDVQVKGNNNTNDSAGDERITSHLFCSFGCEKTGSFNS
                     FCC"
BASE COUNT         1175 a          400 c          518 g          984 t
ORIGIN      
        1 tcatagcatt aaattttttt agatgaagta agcgatatta cgaaatgata tatagaacat
       61 ttcagttata acagtatacg aagtaatcat cttcgtttaa cggacaatag ttaataatca
      121 gttaatttta aattcaatta tagattattt gtgataatac aaacattatt tttgaagatt
      181 tgattaaagt cgtttttatt tttacagaga aaagattgag tatcggtaat tttatcatat
      241 tgttcgaata taaagggaac tatttaaagg taatgaaggt tgcgccaaaa tgaccggttt
      301 tcagcagcat aaatgaataa attgtgtcat ttttaatatt ttatagcatt ttatttttta
      361 atgaacagtt aattttataa ataagtgaaa taatctagaa taaatttgta ttaaaaattt
      421 atatagaaag aaagtgaaac ttatgtttaa gaaaaaaatg ttagctgcaa ctttgtcagt
      481 aggactgatt gcacctttag catctccgat tcaagaatct agagcaaata ctaatattga
      541 aaatattggt gatggtgctg aagtaatcaa acgtacggag gatgtaagta gtaagaaatg
      601 gggcgttact caaaatgtcc aattcgactt tgtaaaagat aaaaaatata acaaagacgc
      661 tttaattgtt aaaatgcaag gttttattaa ttccagaact tcattttcag atgtgaaggg
      721 tagtggatat gaattaacta aacgaatgat ttggccattc caatataata taggactgac
      781 gactaaagat ccaaatgtta gcttaatcaa ttaccttcct aaaaacaaaa tagaaactac
      841 tgatgttggt caaacattag gatataacat tggaggtaat ttccagtcag caccatctat
      901 aggtggcaat ggctcattta attattctaa aacaattagt tatacccaaa agagttatgt
      961 cagtgaagta gacaagcaaa actcaaaatc tgttaaatgg ggtgttaaag caaacgaatt
     1021 tgttacgcct gatggaaaaa aatctgcgca tgatagatat ttattcgtac aaagtccaaa
     1081 tggtccaaca ggttcagcaa gagaatattt tgctcctgat aatcaattgc cacctttagt
     1141 tcaaagtggc tttaatccat cgtttatcac tacactatca catgaaaaag gttcaagtga
     1201 tacgagtgaa tttgaaattt catatggtag aaacttagat attacatatg cgactttatt
     1261 ccctagaact ggtatttacg cagaaagaaa gcataatgca tttgtaaata gaaactttgt
     1321 agttagatat gaagttaatt ggaaaacaca cgaaattaaa gtgaaaggac ataattaata
     1381 tgaaaatgaa aaaattagtc aaatcatcag ttgcttcatc aattgcactg cttttgctat
     1441 cgaatacagt tgatgcagct caacatatca cacctgtaag cgagaaaaaa gtagatgaca
     1501 aaatcacttt atacaaaaca acagcaacat ctgataatga taaattgaat atttctcaaa
     1561 ttttaacgtt taatttcatt aaggataaaa gttatgacaa agatacgtta gtacttaagg
     1621 cagccggaaa cattaattca ggttataaaa agcctaatcc aaaagattac aattactcac
     1681 agttttattg gggcggtaag tataatgttt cggttagttc agaatcaaat gatgctgtaa
     1741 atgttgttga ctatgcacct aaaaatcaaa atgaagaatt ccaagttcaa caaacattag
     1801 gttattctta tggcggagat attaatatat ctaatggctt atcaggtgga ttaaatggat
     1861 caaaatcatt ttcagaaacg ataaattata aacaagaaag ttacagaact acgattgata
     1921 gaaaaacaaa tcataaatca attggctggg gtgttgaggc gcacaaaatt atgaataatg
     1981 gttggggacc atatggtaga gatagttatg acccaacata tggtaatgaa ctgtttttag
     2041 gcggtagaca aagtagttca aatgctggtc aaaatttctt gccaacacat caaatgcctt
     2101 tattggcgcg tggtaacttt aacccagaat ttataagcgt actttctcat aaacaaaatg
     2161 atacaaaaaa atctaaaatc aaagtaactt accaaagaga aatggataga tatactaatc
     2221 aatggaatcg actacactgg gttggtaata actacaaaaa tcaaaataca gtaacgttta
     2281 catctactta tgaagttgac tggcaaaacc atactgttaa attaatcggt acggattcta
     2341 aagaaactaa tcctggagta taactgttag aaatagagga agttaaaccc gaaatttaag
     2401 agttaaacaa aaaatattca caacatatta aaaaaagaag ttacattatt tgtttaaact
     2461 aacaatagta ccataaaatt aatgacgatt tacaaattaa taacaaataa ttattattaa
     2521 attcattgat ttattgtgtg gtaatgatta atatgacaat tgaagtgggt cttaacttca
     2581 acttttaaat agggggtttt atctttggaa aaagttcttg atttagacgt gcaagttaaa
     2641 ggaaataaca acactaatga ttcagcgggt gacgaaagaa taactagcca tcttttttgt
     2701 agctttggtt gtgaaaagac gggtagtttt aacagcttct gttgttaagg atacagcaaa
     2761 tagagcctga aagggctcta tttgtctttt taaatatatt aagtgctaaa aaacggaaac
     2821 agtttattaa tcgccaggta tttttataat tagccttcta tattatcagt tgagtgtttc
     2881 aataaattct gactttggtg atggaaagtt atacttttag ttgtaatata gttttcatga
     2941 cttcctgtta aataagttag gattatgtaa ttagcctcta tctttacata taagtaatga
     3001 caagctttgg ttattatatt gaccctctta gaacatgtca tttaagaaaa taatcaagtc
     3061 attgaaatta tatgttt
//
LOCUS       DALAGD010000170         3076 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.178, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000170 DALAGD010000000
VERSION     DALAGD010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.178"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            65..2908
                     /gene="sdrC"
                     /locus_tag="O2701_002712"
     CDS             65..2908
                     /gene="sdrC"
                     /locus_tag="O2701_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrC"
                     /protein_id="HCZ4294566.1"
                     /translation="MNNKKTATNRKGMIPNRLNKFSIRKYSVGTASILVGTTLIFGLS
                     GHEAKAAEHTNGELNQSKNETTAPSENKTTKKVDSRQLKDNTQTATADQPKVTMSDSA
                     TVKETSSNMQSPQNATANQSTTKTSNVTTNDKSSTTYSNETDKSNLTQAKDVSTTPKT
                     TTIKPRTLNRMAVNTVAAPQQGTNVNDKVHFSNIDIAIDKGHVNQTTGKTEFWATSSD
                     VLKLKANYTIDDSVKEGDIFTFKYGQYFRPGSVRLPSQTQNLYNAQGNIIAKGIYDST
                     TNTTTYTFTNYVDQYTNVRGSFEQVAFAKRKNATTDKTAYKMEVTLGNDTYSEEIIVD
                     YGNKKAQPLISSTNYINNEDLSRNMTAYVNQPKNTYTKQTFVTNLTGYKFNPNAKNFK
                     IYEVTDQNQFVDSFTPDTSKLKDVTDQFDVIYSNDNKTATVDLMKGQTSSNKQYIIQQ
                     VAYPDNSSTDNGKIDYTLDTDKTKYSWSNSYSNVNGSSTANGDQKKYNLGDYVWEDTN
                     KDGKQDANEKGIKGVYVILKDSNGKELDRTTTDENGKYQFTGLSNGTYSVEFSTPAGY
                     TPTTANVGTDDAVDSDGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQ
                     DSTEKGIKGVKVTLQNEKGEVIGTTETDENGKYRFDNLDSGKYKVIFEKPAGLTQTGT
                     NTTEDDKDADGGEVDVTITDHDDFTLDNGYYEEETSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDNDSDSDSDSDSDAGKHTPAKPMSTVKDQHKTAKALPETG
                     SENNNSNNGTLFGGLFAALGSLLLFGRRKKQNK"
BASE COUNT         1229 a          497 c          547 g          803 t
ORIGIN      
        1 agaaatttat atatgcaaaa tattatattt atcaaatttt gatattttaa aggagtatta
       61 ttaaatgaat aataaaaaga cagcaacaaa tagaaaaggc atgataccaa atcgattaaa
      121 caaattttcg ataagaaagt attctgtagg tactgcttca attttagtag ggacaacatt
      181 gatttttggg ttaagtggtc atgaagctaa agcggcagaa catacgaatg gagaattaaa
      241 tcaatcaaaa aatgaaacga cagccccaag tgagaataaa acaactaaaa aagttgatag
      301 tcgtcaacta aaagacaata cgcaaactgc aactgcagat cagcctaaag tgacaatgag
      361 tgatagtgca acagttaaag aaactagtag taacatgcaa tcaccacaaa acgctacagc
      421 taatcaatct actacaaaaa ctagcaatgt aacaacaaat gataaatcat caactacata
      481 tagtaatgaa actgataaaa gtaatttaac acaagcaaaa gatgtttcaa ctacacctaa
      541 aacaacgact attaaaccaa gaactttaaa tcgcatggca gtgaatactg ttgcagctcc
      601 acaacaagga acaaatgtta atgataaagt acatttttca aatattgaca ttgcgattga
      661 taaaggacat gttaatcaga ctactggtaa aactgaattt tgggcaactt caagtgatgt
      721 tttaaaatta aaagcaaatt acacaatcga tgattctgtt aaagagggcg atatatttac
      781 ttttaaatat ggtcaatatt tccgtccagg atcagtaaga ttaccttcac aaactcaaaa
      841 tttatataat gcccaaggta atattattgc aaaaggtatt tatgatagta caacaaacac
      901 aacaacatat acttttacga actatgtaga tcaatataca aatgttagag gtagctttga
      961 acaagttgca tttgcgaaac gtaaaaatgc aacaactgat aaaacagctt ataaaatgga
     1021 agtaacttta ggtaatgata catatagcga agaaatcatt gtcgattatg gtaataaaaa
     1081 agcacaaccg cttatttcaa gtacaaacta tattaacaat gaagatttat cgcgtaatat
     1141 gactgcatat gtaaatcaac ctaaaaatac atatactaaa caaacgtttg ttactaattt
     1201 aactggatat aaatttaatc caaatgcaaa aaacttcaaa atttacgaag tgacagatca
     1261 aaatcaattt gtggatagtt tcacccctga tacttcaaaa cttaaagatg ttactgatca
     1321 attcgatgtt atttatagta atgataataa aacagctaca gtcgatttaa tgaaaggcca
     1381 aacaagcagc aataaacaat acatcattca acaagttgct tatccagata atagttcaac
     1441 agataatgga aaaattgatt atactttaga cactgacaaa actaaatata gttggtcaaa
     1501 tagttattca aatgtgaatg gctcatcaac tgctaatggc gaccaaaaga aatataatct
     1561 aggtgactat gtatgggaag atacaaataa agatggtaaa caagatgcca atgaaaaagg
     1621 gattaaaggt gtttatgtca ttcttaaaga tagtaacggt aaagaattag atcgtacgac
     1681 aacagatgaa aatggtaaat atcagttcac tggtttaagc aatggaactt atagtgtaga
     1741 gttttcaaca ccagccggtt atacaccgac aactgcaaat gtaggtacag atgatgctgt
     1801 agattctgat ggactaacta caacaggtgt cattaaagac gctgacaaca tgacattaga
     1861 tagtggattc tacaaaacac caaaatatag tttaggtgat tatgtttggt acgacagtaa
     1921 taaagatggt aaacaagatt cgactgaaaa aggaattaaa ggtgttaaag ttactttgca
     1981 aaacgaaaaa ggcgaagtaa ttggtacaac tgaaacagat gaaaatggta aataccgctt
     2041 tgataattta gatagtggta aatacaaagt tatctttgaa aaacctgctg gcttaactca
     2101 aacaggtaca aatacaactg aagatgataa agatgccgat ggtggcgaag ttgatgtaac
     2161 aattacggat catgatgatt tcacacttga taatggctac tacgaagaag aaacatcaga
     2221 tagcgactca gattctgaca gcgattcaga ctcagatagc gactcagatt cagatagcga
     2281 ctcagattca gacagcgatt cagacagcga ctcagactca gatagcgatt cagattcaga
     2341 cagcgactca gactcagaca gcgattcaga ctcggatagc gactcagact cagatagcga
     2401 ctcagattcg gatagcgact cagactcaga tagcgattca gattcagata gcgattcgga
     2461 ctcagacagt gattcagatt cagactcaga tagcgactca gattctgaca gcgattcaga
     2521 ctcagacagc gactcagact cagacagtga ttcagattca gacagcgact cagattcaga
     2581 tagcgactca gactcagata gcgactcaga ctcagatagc gactcagact cggatagcga
     2641 ttcagattca gacagcgact cagattcaga tagcgattcg gactcagaca acgactcaga
     2701 ttcagatagc gattcagatt cagatgcagg taaacatact ccggctaaac caatgagtac
     2761 ggttaaagat cagcataaaa cagctaaagc attaccagaa acaggtagtg aaaataataa
     2821 ttcaaataat ggcacattat tcggtggatt attcgcggca ttaggatcat tattgttatt
     2881 cggtcgtcgt aaaaaacaaa ataaataata tgattaactt aaccaggtcc atgtggcctg
     2941 gttttttctg tttagaaatt cccagatata aaacaaaaaa tctttaagga atagatacaa
     3001 tttaatttgt tacagaaatg taattgtcta tgaattagaa aagctaagat attaatttca
     3061 agggcaaata atacta
//
LOCUS       DALAGD010000171         3034 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.179, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000171 DALAGD010000000
VERSION     DALAGD010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.179"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            247..747
                     /locus_tag="O2701_002713"
     CDS             247..747
                     /locus_tag="O2701_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCZ4294567.1"
                     /translation="MTFEEKLSEIYNKIANEISGMIPVEWDQVFTIAYVNDRGGEIVF
                     NYTKPGSDELNYYTYIPREYNVSEKVFYDLWTDLYRLFKKLRNAFKEEDLEPWTSCEF
                     DFTRDGKLNVVFDYVDWMNSEFGPIAKENYYMYKKFGVLPETEYEINKVKEIDQYVKE
                     QDEAEL"
     gene            759..1259
                     /locus_tag="O2701_002714"
     CDS             759..1259
                     /locus_tag="O2701_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCZ4294568.1"
                     /translation="MTFEEKLSQMYNKIASEISGMIPVEWEQVFTIAYVTDQAGEVIF
                     NYTKPGSDELNYYSDIPKDCNVSKDIFKNSWFKVYRMFDELRETFKEEELEPWTSCEF
                     DFTRDGKLNVSFDYIDWVNSEFGPMGREHYYMYKKFGIWPEKEYAINWVEKIKDYVKE
                     QDEAEL"
     gene            1270..1770
                     /locus_tag="O2701_002715"
     CDS             1270..1770
                     /locus_tag="O2701_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin YezG family protein"
                     /protein_id="HCZ4294569.1"
                     /translation="MTFEGKLSQMYNEIANEISGMIPVEWEQVFTIAYVTDQAGEVIF
                     NYTKPDSDELNYYSDIPKDCNVSKDIFKNSWFKVYRMFDELRETFKEEGLEPWTSCEF
                     DFTRDGKLNVSFDYIDWINTEFDQLGRQNYYMYKKFGVIPEMEYEMEEVKEIEQYIKE
                     QEEAEQ"
     gene            1781..2281
                     /locus_tag="O2701_002716"
     CDS             1781..2281
                     /locus_tag="O2701_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCZ4294570.1"
                     /translation="MTFEEKISKLYNEIANEISSMIPVEWEKVYTMAYIDDGGGEVFF
                     NYTKPGSDDLNYYTDIPKEYNISVQVFDDLWMDLYDLFEELRDLFKEEGLEPWTSCEF
                     DFTSEGKLKVSFDYIDWINTEFDQLGRENYYMYKKFGVLPEMEYEMEEIKEIDQYIKE
                     QDEAEI"
     gene            2292..2780
                     /locus_tag="O2701_002717"
     CDS             2292..2780
                     /locus_tag="O2701_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin YezG family protein"
                     /protein_id="HCZ4294571.1"
                     /translation="MTFEEKLSQMYNEIANEISGMIPVEWEKVYTIAYVDDEGGEVVF
                     NYTKPGSEDLNYYSDIPKDCNVSKDIFKNSWFKVYRMFDELRETFKKEDLEPWTSCEF
                     DFTRKGNLKVSFDYIDWIKLGFGPSGKENYYMYKKFGILPDMEYEMEEIRAVEKYVKE
                     QE"
BASE COUNT         1194 a          292 c          614 g          934 t
ORIGIN      
        1 aaataatgag taggagtttt agggctatta tttacaaata atataatact gcaaagactc
       61 gaaattattt agaattccag ttatcgtaac aatagttgca gttattttag ttccattagt
      121 gtatttaggt ttaaaatgag taatattaat ttgaagaagc attaagcgac gtagaggtgt
      181 tgtttagtgc tttttattta acggaaatga aaccaatatc tattttgaaa atataggggc
      241 gataacatga ctttcgaaga aaaactaagt gaaatataca acaagattgc gaatgagatt
      301 agtgggatga taccagtaga atgggaccaa gtatttacga tagcttatgt aaatgataga
      361 ggtggagaga tcgtttttaa ttatactaaa ccaggtagcg atgaattgaa ttattacaca
      421 tatatcccta gagagtataa tgtctctgaa aaagtatttt atgatttgtg gacggattta
      481 tatagattgt ttaagaagtt aagaaacgca tttaaagaag aagatttaga accatggaca
      541 tcatgtgaat ttgactttac aagagatggt aaattgaatg ttgtatttga ttatgttgat
      601 tggatgaatt cagaatttgg tccaatagca aaagaaaatt attatatgta caaaaagttt
      661 ggtgttttac cagaaacgga atatgaaatt aataaagtta aagaaatcga tcaatatgtt
      721 aaagagcaag atgaagctga actatagggg gagataacat gactttcgaa gagaaactaa
      781 gtcaaatgta caataaaatt gcaagtgaga tcagtggaat gataccagta gaatgggagc
      841 aagtatttac aatagcttat gtaactgatc aagctgggga agtcattttt aattatacta
      901 aaccaggtag tgatgaatta aattattatt cagacatacc taaagattgc aatgtctcaa
      961 aagatatttt taagaattca tggtttaaag tttatcgaat gtttgatgag ttaagagaaa
     1021 cttttaaaga agaagagctt gaaccatgga catcatgcga atttgacttt acaagagatg
     1081 gcaaattgaa tgtatctttt gattatattg attgggtgaa ttcagaattt ggaccaatgg
     1141 gaagagaaca ttattatatg tataaaaaat ttggaatttg gcctgaaaaa gaatatgcca
     1201 taaattgggt tgaaaaaata aaagattatg ttaaagagca agatgaagct gaactatagg
     1261 ggagataata tgactttcga aggaaaacta agtcaaatgt acaacgagat tgcgaatgag
     1321 attagtggga tgataccggt agaatgggag caagtattta caatagctta tgtaactgat
     1381 caagctggag aagtcatctt taattatact aaaccagata gtgatgaatt aaattattat
     1441 tcagacatac ctaaagattg caatgtctca aaagatattt ttaagaattc atggtttaaa
     1501 gtttatcgaa tgtttgatga gttaagagaa acttttaaag aagaagggct tgaaccatgg
     1561 acatcatgcg aatttgactt tacaagagat ggcaaattga atgtatcttt tgattatata
     1621 gattggataa atacagagtt tgatcaattg ggccgtcaaa attattatat gtacaaaaaa
     1681 tttggggtta taccagaaat ggaatatgaa atggaagaag ttaaagaaat cgaacaatat
     1741 attaaagagc aagaagaagc tgaacaatag aggtgataac atgactttcg aagagaaaat
     1801 aagcaaatta tataatgaga ttgcgaatga gattagcagt atgataccgg tagagtggga
     1861 aaaagtatat acaatggctt atatagatga tggaggaggt gaagtattct ttaattatac
     1921 taaaccaggt agtgatgact tgaattatta caccgatata cctaaggagt ataacatctc
     1981 tgtgcaagta tttgatgatt tatggatgga tttatatgat ttgtttgagg aattaagaga
     2041 tttatttaaa gaagaaggac ttgaaccatg gacatcatgt gaatttgact ttacaagcga
     2101 aggtaaatta aaagtttcat ttgattatat agattggata aatacagagt ttgatcaatt
     2161 aggccgtgaa aattattata tgtataaaaa atttggggtt ttaccagaaa tggaatatga
     2221 aatggaagaa attaaagaaa tcgatcaata tattaaagag caagatgaag ctgaaatata
     2281 ggggagataa catgactttc gaagaaaagc taagtcaaat gtacaatgaa attgcaaatg
     2341 aaatcagtgg aatgatacca gtagaatggg aaaaagtata tacaattgcc tacgtagatg
     2401 atgaaggtgg agaggttgtt tttaattata ctaaaccagg aagtgaagat ttgaattatt
     2461 attcagatat tcctaaagat tgcaatgtct caaaagatat ttttaagaat tcatggttta
     2521 aagtttatcg aatgtttgat gagttaagag aaacttttaa aaaagaagat ttagaaccgt
     2581 ggacatcatg tgaatttgac tttacaagaa agggaaattt aaaagtatca tttgattata
     2641 tagattggat taaattaggt tttggcccat caggaaagga aaactactat atgtacaaaa
     2701 aatttggtat tttaccagat atggaatatg aaatggaaga aattcgagca gtagagaagt
     2761 atgttaaaga gcaagagtag cagacatgtt ataaaagact gtgcaaaatc accctcgttt
     2821 tacatttgat tcaaagaaga aggtaaaaga taagattatt tgcaacttaa aaagtcaatt
     2881 agcttatcgg tatccataca tcatggataa atgagttcaa ctaattaaca aatcacgata
     2941 taaatttaaa attgtcatta attgaaaagg ttattgtagt gtattttata aagttgatgt
     3001 acatcgactt attttttaaa acttatatca aaaa
//
LOCUS       DALAGD010000172         3020 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.180, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000172 DALAGD010000000
VERSION     DALAGD010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.180"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..173
                     /locus_tag="O2701_002718"
     CDS             <1..173
                     /locus_tag="O2701_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294572.1"
                     /translation="HLINGEVIKVQEHMKDAESKTLHIIYPKDRVVSLDHVLYFDINV
                     KGEKNNDSPYPS"
     gene            complement(170..346)
                     /locus_tag="O2701_002719"
     CDS             complement(170..346)
                     /locus_tag="O2701_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294573.1"
                     /translation="MNEEKLKMILLLLEDIPRDEWNRLVNEVNNQYSYQADKVGLASD
                     NCQQIANNYKHYGF"
     gene            complement(430..621)
                     /locus_tag="O2701_002720"
     CDS             complement(430..621)
                     /locus_tag="O2701_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ4294574.1"
                     /translation="MPEQLSVRKWRLVRDLKQQEVADILGVNAKTVGHWEKDDTNLSN
                     VTVYALAKLYDIEVDQIKV"
     gene            782..1111
                     /locus_tag="O2701_002721"
     CDS             782..1111
                     /locus_tag="O2701_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ4294575.1"
                     /translation="MENNKVRKILSENLQELMNDKNIDQRELAEAIGVSQPTVSNWIQ
                     QTKYPRIKRIQQLADYFNVPKSRITESKKDIHQETIAAHFDKEGLTEEEIEEVNRFIE
                     WVRNRDK"
     gene            1124..1585
                     /locus_tag="O2701_002722"
     CDS             1124..1585
                     /locus_tag="O2701_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCZ4294576.1"
                     /translation="MGLYEELCINNEKIKIEETDQLPNFQPGCYMNGKIYIRRNLSEV
                     RKAEVLYEQLAHHKLTYGNILDQTKWINRKFENYARRHGFTSAVPLHEIVEAHNYGVR
                     NLYELSEYLQLSESYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI"
     gene            1603..2037
                     /locus_tag="O2701_002723"
     CDS             1603..2037
                     /locus_tag="O2701_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294577.1"
                     /translation="MKKVIGLLLVSTLALTACGEKEKPKKEENKKSQTQNHKDSKPKT
                     QQEKMKKVEDKNPPNNSIQNNSNNQNQSQNNQLNNNSDPSNNTPANINENDSQNTNLN
                     DEYVVSPGWTKDEQAKAFEEYKKGKEEEARAGASAVPGANIN"
     gene            2066..2461
                     /locus_tag="O2701_002724"
     CDS             2066..2461
                     /locus_tag="O2701_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294578.1"
                     /translation="METNKTIDLMNYVEFPKRYTEAKGKLVAQPITTINSARRVENED
                     MTVCYILDQDDDVMDFIFDRDIITVYCPENGTATDEYFCEIIFNSDDTFTLKRLSNYV
                     TIKDISYPMSKINDVNITGKVVRLFRDFK"
     gene            2551..2697
                     /locus_tag="O2701_002725"
     CDS             2551..2697
                     /locus_tag="O2701_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294579.1"
                     /translation="MNGGENFMVDKNKKQETTRSNPLNKSFEKSGASEKLKSTLSEKA
                     KKKD"
     gene            complement(2694..>3020)
                     /locus_tag="O2701_002726"
     CDS             complement(2694..>3020)
                     /locus_tag="O2701_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCZ4294580.1"
                     /translation="VKFIIWLINITRKFMKLDCISLLDKRDKLFNNNGKPVFIVIKDF
                     ENRIIEEGELKTYNSAGSDFDLLEVERQDFKVSDLPSNDELYIKHTLVDLKQQIKLDL
                     YLMNEY"
BASE COUNT         1180 a          458 c          476 g          906 t
ORIGIN      
        1 tacatttaat taatggtgaa gttattaaag tacaagaaca tatgaaagat gctgaaagca
       61 aaactctaca catcatttat ccaaaagata gagttgtcag tttagatcat gttttgtatt
      121 tcgacattaa cgttaaaggt gaaaagaata acgatagtcc ttatccatct taaaatccat
      181 agtgcttata gttatttgct atttgttgac agttatcact ggcaagtcct accttgtcag
      241 cttggtaact gtattgatta tttacttcat ttactaatcg attccattcg tctcttggta
      301 tatcttcaag aagtaagaga atcatcttta atttttcttc gttcatttgt tgttcctcct
      361 tttaagttgt ttatgtccat ttgttacatt acctaaaagt tataaagagt tatacgaagg
      421 tcaaaaaatt tagaccttta tctggtctac ctcaatatca tataactttg ctaaagcgta
      481 aactgtaaca ttacttaaat tagtgtcatc cttttcccaa tgaccaactg tctttgcatt
      541 gacgcccaat atatctgcta cttcttgctg tttcaagtcc cttacaagtc tccattttct
      601 tacggataat tgttccggca tgtcgtccac ctccctttca cacgtataac tttatattac
      661 tttgtattac ttgtcaacag ataaagttat atttcttcat aaaaagttat aaaaagtatt
      721 gtaaagtaat gtcggttatg gtaagttaat attacattaa gtaatattaa aggagacaac
      781 tatggagaat aataaagtca gaaaaatttt atctgaaaac cttcaagaac ttatgaatga
      841 taaaaatatt gatcagagag aacttgctga agctattgga gtttctcaac ctacagtctc
      901 caattggatt caacaaacta aatatccacg aattaaaaga attcaacaac ttgcagatta
      961 cttcaatgta ccgaaatcaa gaattactga atcaaaaaaa gatatacatc aagaaacaat
     1021 tgctgctcat tttgataagg agggattaac tgaagaagag attgaagaag taaatagatt
     1081 cattgaatgg gttagaaata gagacaaata aagggtgttt atattgggat tatacgaaga
     1141 actttgcata aataacgaaa aaataaagat agaagaaact gaccagcttc caaatttcca
     1201 acctggatgc tatatgaacg gaaaaattta tataaggcgt aatttatcag aagtacgtaa
     1261 agcagaagtg ttatatgagc aacttgccca ccacaagttg acgtatggca acattttaga
     1321 ccaaacaaaa tggattaata gaaaatttga aaattatgca cgtagacatg gttttacttc
     1381 agctgtaccg ctacatgaaa ttgtagaagc tcacaactac ggtgttcgta atttgtacga
     1441 gttgtcagag tatctgcagt tgagtgaatc atacatacta gaagctatag aacaatataa
     1501 aaagatatat ggtattggaa ctcactatgg cgagtattct attacgtttg agccgttgag
     1561 agtttttaaa tataaggaaa tataaacaaa ggagagatac atatgaaaaa agtaatcgga
     1621 ctgctactag taagtacatt agctttaaca gcttgtggtg aaaaagaaaa accaaaaaaa
     1681 gaagaaaata aaaagtcaca aacacaaaat cacaaagata gcaaaccaaa aacgcaacaa
     1741 gaaaaaatga aaaaagttga agataaaaat ccacctaata atagcataca aaataattca
     1801 aacaatcaaa accaatcaca aaacaatcaa cttaataata attcagatcc atctaataat
     1861 actcctgcaa atataaatga aaacgattca caaaatacta atttaaatga tgagtatgtc
     1921 gtttcgcctg gctggactaa agatgaacag gctaaagctt ttgaagagta caaaaaagga
     1981 aaagaagagg aagcaagagc tggtgctagc gcagtaccag gagccaatat taactaataa
     2041 aacaatataa gaaagaagag ctaatatgga aacaaataaa acaatcgatt taatgaatta
     2101 tgtggaattt ccaaaaagat acacagaggc aaaaggcaaa ttagttgcac aaccaataac
     2161 tactataaat agcgcaagaa gagttgaaaa tgaagatatg actgtttgct acattttaga
     2221 tcaggatgat gatgtaatgg actttatctt tgacagagat ataattactg tttactgtcc
     2281 tgaaaacgga actgcgactg atgaatattt ttgtgaaatt atatttaact cagatgacac
     2341 atttacccta aagcgattat ctaattacgt taccattaaa gatataagct acccaatgtc
     2401 aaaaataaat gacgtaaaca ttacgggcaa agtcgtcaga ttatttagag attttaaata
     2461 aacttggctt taattacgat taaaagtacc tatatagcgt gacgagaaaa aggattaaaa
     2521 aaaatcaaaa aacgcctact agtgtagacg ttgaatggtg gtgagaattt tatggtagat
     2581 aaaaacaaaa aacaagaaac tactcgtagt aacccattaa acaaaagttt tgaaaagtca
     2641 ggcgccagcg aaaaattaaa aagcacttta tcagaaaaag ctaagaaaaa agattagtat
     2701 tcattcatta aatataaatc caatttaatt tgttgtttaa ggtctacaag cgtatgttta
     2761 atatacaatt catcgtttga cggtaaatca gatactttga aatcttgtcg ctcaacttct
     2821 agtaaatcga aatcgctacc agctgaatta taggttttaa gttcaccctc ttcaatgatt
     2881 ctgttttcaa agtcttttat aactataaat actggtttac cgttgttatt aaacaacttg
     2941 tctcttttgt ctaataagct tatacaatcc aatttcataa actttctggt tatattaatt
     3001 aaccagataa taaatttaac
//
LOCUS       DALAGD010000173         2976 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.181, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000173 DALAGD010000000
VERSION     DALAGD010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.181"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(187..2796)
                     /gene="adhE"
                     /locus_tag="O2701_002727"
                     /gene_synonym="adhC"
     CDS             complement(187..2796)
                     /gene="adhE"
                     /locus_tag="O2701_002727"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCZ4294581.1"
                     /translation="MLTIPEKENRGSKEQEVAIMIDALADKGKKALEALSKKSQEEID
                     HIVHQMSLAAVDQHMVLAKLAHEETGRGIYEDKAIKNLYASEYIWNSIKDNKTVGIIG
                     EDKEKGLTYVAEPIGVICGVTPTTNPTSTTIFKAMIAIKTGNPIIFAFHPSAQESSKR
                     AAEVVLEAAMKAGAPKDIIQWIEVPSIEATKQLMNHKGIALVLATGGSGMVKSAYSTG
                     KPALGVGPGNVPSYIEKTAHIKRAVNDIIGSKTFDNGMICASEQVVVIDKEIYKDVTN
                     EFKAHQAYFVKKDELQRLENAIMNEQKTGIKPDIVGKSAVEIAELAGIPVPENTKLII
                     AEISGVGSDYPLSREKLSPVLALVKAQSTKQAFQICEDTLHFGGLGHTAVIHTEDETL
                     QKDFGLRMKACRVLVNTPSAVGGIGDMYNELIPSLTLGCGSYGRNSISHNVSATDLLN
                     IKTIAKRRNNTQIFKVPAQIYFEENAIMSLTTMDKIEKVMIVCDPGMVEFGYTKTVEN
                     VLRQKTEQPQIKIFSEVEPNPSTNTVYKGLEMMVDFQPDTIIALGGGSAMDAAKAMWM
                     FFEHPETSFFGAKQKFLDIGKRTYKIGMPENATFICIPTTSGTGSEVTPFAVITDSET
                     NVKYPLADFALTPDVAIIDPQFVMSVPKSVTADTGMDVLTHAMESYVSVMASDYTRGL
                     SLQAIKLTFEYLKSSVEKGDKVSREKMHNASTLAGMAFANAFLGIAHSIAHKIGGEYG
                     IPHGRANAILLPHIIRYNAKDPQKHALFPKYEFFRADTDYADIAKFLGLKGNTTEALV
                     ESLAKAVYELGQSVGIEMNLKSQGVSEEELNESIDRMAELAFEDQCTTANPKEALISE
                     IKDIIQTSYDYKQ"
BASE COUNT          845 a          561 c          470 g         1100 t
ORIGIN      
        1 tttgtgaaat gagtatcaat cttaatattt tctttattta aaaaaatgat tgtctagttt
       61 gtatctctct gaagatttgg caataaataa aagccgataa ccgtataatg attatcgact
      121 taaagtttat gtggcatttt ttacttttgt aatttcaggt gagttagatg attattatca
      181 gatagattat tgcttataat catatgatgt ttgaatgata tctttgattt cactgattag
      241 tgcttcttta ggattagcag ttgtacattg atcttcaaat gcgagctctg ccattctatc
      301 aattgactca tttaattctt cttcagacac accttgtgat ttcaaattca tttcaattcc
      361 gactgattga cctaattcgt agacagcttt agctaatgat tctacgagtg cttctgtcgt
      421 attacctttt aatcctaaga atttggcaat atctgcataa tctgtatctg ctctgaagaa
      481 ctcatattta gggaataatg catgtttttg cgggtctttg gcattataac ggataatatg
      541 cggtagtaat atcgcattcg ctctaccatg cggaatacca tattcgccac caattttatg
      601 cgcaattgag tgtgcaatgc ctaagaatgc atttgcaaat gccataccag ccaaagttga
      661 tgcgttatgc attttctctc ttgaaacttt atcacccttt tcaacagatg attttaaata
      721 ttcgaacgtc aatttaatcg cttgtagact caaacctctt gtgtagtctg aagccattac
      781 agatacatat gattccattg catgcgttag tacatccatt cctgtatctg ctgtaacgct
      841 ttttggcaca ctcatcacaa attgagggtc aataattgca acgtcaggtg ttaaagcaaa
      901 atcagccaac ggatatttta catttgtttc actatctgtg ataactgcaa atggtgttac
      961 ttctgaacct gtacctgatg tcgtagggat acaaatgaac gtcgcatttt caggcatgcc
     1021 tattttataa gtacgtttac cgatgtctag gaacttttgt ttagcaccga agaatgatgt
     1081 ctcagggtgt tcaaagaaca tccacattgc ttttgcagca tccatcgctg aaccaccacc
     1141 aagtgcaatg attgtatccg gttggaaatc aaccatcatt tccagacctt tatatactgt
     1201 attagttgat gggttcggtt cgacttcgct aaatatttta atttgaggct gttccgtttt
     1261 ttgtcttaat acattctcaa ctgtttttgt ataaccgaat tctaccatac cagggtcaca
     1321 gacaatcatc actttttcaa tcttgtccat tgttgttaga ctcatgattg cattttcttc
     1381 aaaataaatt tgagcaggca ccttgaaaat ttgagtatta ttacgtcgtt tagcaatcgt
     1441 tttaatgttt aataaatctg tcgcactaac attatgtgaa attgagtttc taccgtagga
     1501 accacaacct aatgttaaag acggaatcaa ttcgttatac atatcaccaa tacctccaac
     1561 cgctgatggt gtatttacaa gtacacgaca agctttcatt cttagtccaa aatctttttg
     1621 taatgtttca tcttctgtat ggataacggc tgtgtgtcct aatccaccaa aatgtagtgt
     1681 gtcttcacaa atttgaaatg cttgttttgt agattgggct tttactaagg ctaatactgg
     1741 agataatttt tcacgagata acggatagtc tgaacctaca ccgctaattt cggctatgat
     1801 aagttttgta ttttcgggga caggtatacc tgctaattca gctatttcaa ctgcagattt
     1861 accgacaata tcaggcttaa tacctgtttt ttgttcattc ataattgcat tttctaagcg
     1921 ttgtaattca tcttttttaa caaagtatgc ttgatgtgct ttaaattcat tagtaacatc
     1981 tttataaatt tctttatcaa tgactacaac ttgttcagaa gcacaaatca taccattatc
     2041 aaatgttttt gaaccaatga tatcatttac tgcacgttta atgtgtgctg ttttttcaat
     2101 gtaagacggc acgttacctg gtcccacacc taatgccggt ttgccagttg aatatgcaga
     2161 cttaaccatg cccgaaccac ctgttgctag aactaatgca atacctttgt gattcattaa
     2221 ttgttttgtt gcttcgatag aaggcacttc aatccactga ataatatctt taggtgcacc
     2281 tgccttcatt gccgcttcta atacaacttc tgctgcacgc ttcgacgatt cttgtgcact
     2341 tggatggaat gcaaaaatga ttggatttcc tgtcttaatt gcaatcatcg ctttaaaaat
     2401 agttgtcgac gtaggatttg ttgttggcgt aacaccacaa ataacaccaa ttggttccgc
     2461 tacatacgtt aatccttttt ctttatcttc accaataatc cctactgtct tattgtcttt
     2521 tattgaattc catatatatt cagaagcgta taaattttta atcgctttat cttcgtatat
     2581 acctcttcca gtttcttcat gtgctaattt tgctagcacc atatgttgat caacagctgc
     2641 taagctcatt tgatgaacaa tatgatcaat ttcttcttgt gactttttag ataatgcttc
     2701 taatgctttt ttccctttgt cagctagagc atcaatcata attgccactt cttgttcttt
     2761 cgatccacga ttttcttttt caggtatagt taacatatac aaccactcct ttatactttg
     2821 tgaattattt cacaaacatt atagtacatg tctctcagga tataaagaaa attctataca
     2881 aaaaagttta atttcgaata ttatttgaac aaatatcaaa ttttaaaata aatgttttca
     2941 tgaaatcatt gttatttcgg tgtttttaga atgatt
//
LOCUS       DALAGD010000174         2968 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.182, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000174 DALAGD010000000
VERSION     DALAGD010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.182"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            25..768
                     /locus_tag="O2701_002728"
     CDS             25..768
                     /locus_tag="O2701_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCZ4294582.1"
                     /translation="MTRLWASLLTVIIYILSQFLPLLIVKKLPFVQYSGIELTKAVIY
                     IQLVLFLIAATTIILINLKIKNPTKLELEVKEPKKYIIPWALLGFALVMIYQMVVSIV
                     LTQIYGGQQVSPNTEKLIIIARKIPIFIFFVSIIGPLLEEYVFRKVIFGELFNAIKGN
                     RIVAFIIATTVSSLIFALAHNDFKFIPVYFGMGVIFSLAYVWTKRLAVPIIIHMLQNG
                     FVVIFQLLNPEALKKATEQANFIYHIFIP"
     gene            complement(793..2046)
                     /locus_tag="O2701_002729"
     CDS             complement(793..2046)
                     /locus_tag="O2701_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294583.1"
                     /translation="MSYHWFKKMLLSTSILILSSSSLGLATHTVEAKDNLNGEKPTTN
                     LNHNITSPSVNSEMNNNETGTPHESNQTGNEGTGSNSRDANPDSNNVKPDSNNQNPST
                     DSKPDPNNQNPSPNPKPDPDNPKPKPDPKPDPDKPKPNPDPKPDPDNPKPNPDPKPDP
                     NKPNPDPKPDPDKPKPNPNPKPDPNKPNPNPSPDPDQPGDSNHSGGSKNGGTWNPNAS
                     DGSNQGQWQPNGNQGNSQNPTGNDFVSQRFLALANGAYKYNPYILNQINKLGKDYGEV
                     TDEDIYNIIRKQNFSGNAYLNGLQQQSNYFRFQYFNPLKSERYYRNLDEQVLALITGE
                     IGSMPDLKKPEDKPDSKQRSFEPHEKDDFTVVKKQEDNKKSASTAYSKSWLAIVCSMM
                     VVFSIMLFLFVKRNKKKNKNESQRR"
     gene            2243..2869
                     /locus_tag="O2701_002730"
     CDS             2243..2869
                     /locus_tag="O2701_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCZ4294584.1"
                     /translation="MGLFKKDKKAMTFDNAMEERRSIYNLKDSISISDDELESMIAHA
                     VKHVPSSFNSQSTRIVLLLNDNNNKFWDNTKAILKEVMGENRDFEPTEQKIDNFKHSY
                     GTILFYEDQDVVSGLQEQMPNYYDNFAIWSTQTNAMHQFAIWTALATKGIGASLQHYN
                     PLVDEMTSNEFNIPKSWKLIAQMPFGDIREAAGEKTFNPVEDRFVIKK"
BASE COUNT          919 a          449 c          508 g         1092 t
ORIGIN      
        1 taaacaaata tttggaggaa aattatgaca agattatggg catcattgct aactgttatt
       61 atttatatat tgtctcaatt tttaccgctt ctcattgtaa aaaaattacc atttgtacaa
      121 tatagtggca tagaactgac taaagcagtc atttacatac aacttgttct atttttaatc
      181 gccgccacga cgattatttt aattaattta aaaattaaaa atccaacaaa attagaatta
      241 gaagttaaag aacctaaaaa atatatcatt ccatgggcat tgcttggatt tgcattggta
      301 atgatttatc aaatggtagt gagcattgta ttaacgcaaa tttatggtgg acaacaagta
      361 agtcctaata cagaaaagct aattattatt gctcgaaaaa tacctatatt tatcttcttt
      421 gtatctatta ttggtccttt attagaagaa tatgtattca gaaaagtaat ctttggagaa
      481 ttatttaatg cgattaaagg taatcgtatc gtggcattta ttattgctac aacagtaagt
      541 tcattaatat ttgcattagc acataatgat ttcaaattta ttccagttta ttttggtatg
      601 ggtgtcattt tttcattagc atatgtttgg acaaaacggc ttgctgttcc aattattatc
      661 catatgttac aaaacggatt tgtcgttata ttccaattac ttaatcctga ggctttgaaa
      721 aaagccacgg aacaagcgaa ttttatatat cacattttta ttccataata aaaaaaacga
      781 atcacacacg gattatcgtc gctgtgattc gtttttattt ttctttttat ttcgctttac
      841 aaataagaat agcatgattg aaaataccac catcatagaa catacaattg ctagccaact
      901 tttactatat gcagttgacg cacttttctt attatcttct tgttttttaa ctactgtaaa
      961 atcgtctttt tcatgcggtt caaatgagcg ttgttttgaa tccggcttat cttcgggctt
     1021 tttcaaatct ggcattgatc caatttcacc agtaattaat gcgagtactt gttcatctaa
     1081 attacgatag tacctttctg atttcaatgg attgaaatat tggaatctaa agtaattcga
     1141 ttgctgttgt aatccattta aatatgcatt tccgctgaaa ttttgttttc gaataatatt
     1201 ataaatgtct tcatcagtaa cttctccata atctttgccc aacttattaa tttgatttaa
     1261 aatatacgga ttatacttgt aagccccatt tgccaaggct aaaaatcgtt gggatacaaa
     1321 atcattacca gtaggatttt gtgagtttcc ttgattccca tttggttgcc attgaccttg
     1381 attagatcca tctgaagcat ttgggttcca tgtcccccca tttttcgagc caccagaatg
     1441 attggaatcc ccaggttgat cgggatctgg tgacgggtta ggatttggct tattagggtc
     1501 tggttttgga ttcggatttg gctttggttt atctggatct ggttttggat ccggatttgg
     1561 cttattaggg tctggttttg gatctggatt tggtttcggg ttatctggat ctggttttgg
     1621 atccggattt ggctttggtt tatctgggtc tggttttgga tccggttttg gtttcgggtt
     1681 atctggatct ggtttaggat tcggacttgg gttttggtta tttgggtctg gttttgaatc
     1741 tgtacttggg ttttggttgt ttgagtctgg cttcacatta ttcgaatcag gattagcatc
     1801 acgactattc gaacctgttc cttcattacc cgtttgattt gattcgtgag gtgtcccagt
     1861 ctcattatta ttcatttcac tatttactga tggtgaagtt atattatgat tcaaattagt
     1921 agttggtttt tctccattta agttatcctt tgcttcaact gtgtgcgttg caagccctaa
     1981 actactacta cttaaaatta aaatacttgt tgaaagtaac attttcttaa accaatgata
     2041 tgacatttca cgagacacct ccctttttca tgtaagtatt aatctaagta taataaatat
     2101 gcgttgaaaa tgcatttata tttggaattt gaaaatgaat attaatattc ttgaaatttt
     2161 cgtaaatatt gctttacaag tacgagtata aattttatac ttagtctatt aagtattctt
     2221 tactaaagaa ggagcaataa ttatgggctt atttaaaaaa gataaaaaag caatgacttt
     2281 tgacaatgca atggaagaaa gacgttcaat atataatttg aaagattcga tatcaattag
     2341 cgatgacgaa ttagaatcaa tgattgcaca tgcagttaaa catgttcctt catcatttaa
     2401 ttctcaatca actagaattg ttctcttgtt aaatgacaac aataataaat tttgggataa
     2461 cacaaaagca attttaaaag aagtcatggg agagaatcgt gatttcgagc caactgaaca
     2521 aaaaattgat aacttcaaac attcatacgg tacaattttg ttttatgaag atcaagatgt
     2581 tgtaagtggt ttacaagaac aaatgccaaa ctactatgat aattttgcaa tatggtcaac
     2641 tcaaacaaat gccatgcatc aatttgcaat ttggactgca ttagctacta aaggcatagg
     2701 tgcttcatta caacactata atccacttgt tgatgagatg acttcaaatg aatttaatat
     2761 tcctaaaagt tggaaactta ttgctcaaat gccatttggt gatattcgtg aagctgctgg
     2821 cgaaaaaaca tttaatccag tggaagatcg tttcgtaata aaaaaataat caatatattg
     2881 ttaggaatgg gacagaaatg ataaagaatc attagttatt cattatgtag tagttcttac
     2941 acattagccg ctgctaatgt gcacttaa
//
LOCUS       DALAGD010000175         2923 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.183, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000175 DALAGD010000000
VERSION     DALAGD010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.183"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(99..746)
                     /locus_tag="O2701_002731"
     CDS             complement(99..746)
                     /locus_tag="O2701_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCZ4294585.1"
                     /translation="MKFDNYIFDFDGTLADTKKCGEVATQSAFKACGLTEPSSKEITH
                     YMGIPIEESFLKLADRPLDEAALAKLIDTFRHTYQSIEKDYIYEFAGITEAITSLYNQ
                     GKKLFVVSSKKSDVLERNLSAIGLNHLITEAVGSDQVSAYKPNPEGIHTIVQRYNLNS
                     QQTVYIGDSTFDVEMAQRAGMQSAAVTWGAHDARSLLHSNPDFIINDPSEINTVL"
     gene            complement(859..1407)
                     /gene="hxlB"
                     /locus_tag="O2701_002732"
     CDS             complement(859..1407)
                     /gene="hxlB"
                     /locus_tag="O2701_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HCZ4294586.1"
                     /translation="MAKFSDYQLILDELKMTLSHVEADEFSTFASKILHAEHIFVAGK
                     GRSGFVANSFAMRLNQLGKQAHVVGESTTPAIKSNDVFVIISGSGSTEHLRLLADKAK
                     SVGADIVLITTNKDSAIGNLAGTNIVLPAGTKYDEQGSAQPLGSLFEQASQLFLDSVV
                     MGLMTEMNVTEQTMQQNHANLE"
     gene            complement(1409..2041)
                     /gene="hxlA"
                     /locus_tag="O2701_002733"
     CDS             complement(1409..2041)
                     /gene="hxlA"
                     /locus_tag="O2701_002733"
                     /EC_number="4.1.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hexulose-6-phosphate synthase"
                     /protein_id="HCZ4294587.1"
                     /translation="MELQLAIDLLNKEDAAELANKVKDYVDIVEIGTPIIYNEGLPAV
                     KHMADNISNVKVLADMKIIDAADYEVSQAIKFGADVITILGVAEDASIKAAIEEAHKN
                     NKQLLVDMIAVQDLEKRAKELDEMGADYIAVHTGYDLQAEGQSPLESLRTVKSVIKNS
                     KVAVAGGIKPDTIKDIVAESPDLVIVGGGIANADDPVEAAKQCRAAIEGK"
     gene            complement(2118..2876)
                     /gene="nagB"
                     /locus_tag="O2701_002734"
     CDS             complement(2118..2876)
                     /gene="nagB"
                     /locus_tag="O2701_002734"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCZ4294588.1"
                     /translation="MKVLNLGSKKQASFYVACELYKEMAFNQHCKLGLATGGTMTDLY
                     EQLVKLLNKNQLNVDNVSTFNLDEYVGLTASHPQSYHYYMDDMLFKQYPYFNRKNIHI
                     PNGDADDMNAEASKYNDVLEQQGQRDIQILGIGENGHIGFNEPGTPFDSVTHIVDLTE
                     STIKANSRYFKNEDDVPKQAISMGLANILQAKRIILLAFGEKKRAAITHLLNQEISVD
                     VPATLLHKHPNVEIYLDDEACPKNVAKIHVDEMD"
BASE COUNT          875 a          567 c          414 g         1067 t
ORIGIN      
        1 ttattttaaa ttcctaatct aaagcgaaat atttctaagt ataatctaat atttatgaat
       61 atgcatttta accatggtat tctctgtttt aacaagtttt ataatacggt attaatttct
      121 gatggatcat taataataaa atccggattt gaatgaagta atgaccttgc atcatgtgca
      181 ccccaagtga cagctgcaga ttgcatacca gcacgttgtg ccatctcaac atcaaacgtt
      241 gaatcaccaa tatacaccgt ttgttggcta tttaaattgt agcgttgcac aattgtgtgt
      301 atgccttcag gatttggttt atatgcactt acttgatcgg atccaacagc ttcggtaatc
      361 aagtgattta atccaatagc cgataaattt ctttctaata catcactctt cttactagac
      421 accacgaaaa gttttttccc ttggttatac aaacttgtaa tggcttcagt tatacccgca
      481 aattcataaa tatagtcctt ttcaatagat tgatatgtat gtctaaatgt atcgattaac
      541 tttgctaatg ctgcttcatc taatggtcgg tctgctaatt ttaaaaatga ttcttcaata
      601 ggtattccca tataatgcgt tatttcttta gatgatggtt ccgttaagcc acatgcttta
      661 aatgcacttt gtgttgctac ttcaccacat tttttcgtgt ctgccaacgt accatcaaaa
      721 tcaaaaatat aattgtcaaa cttcaatatt aacaccccta ttcgtttatt attgatatta
      781 tactataatt tattatttaa ataaaaaaat aatcgataat atttctgcct aggcatcata
      841 ttatcgacta tctttatttt attctaaatt agcatgattt tgttgcatcg tttgttccgt
      901 aacattcatt tcagtcatca atcccattac aacactatct aaaaataatt gagatgcttg
      961 ttcaaacaaa cttcctaatg gttgtgccga gccttgttca tcatattttg tacctgcagg
     1021 caaaacgatg ttcgtcccag ctagattgcc tattgcagaa tctttatttg tagtaattaa
     1081 tacgatgtca gcacctactg attttgcttt gtctgctaat aatcttaaat gttccgtgga
     1141 acctgaacca gagataatta caaatacatc attcgactta atcgcaggtg tcgttgattc
     1201 tccaacaaca tgtgcctgtt tgccgagctg atttaagcgc attgcaaaac tattcgccac
     1261 gaatcctgaa cgtcctttgc cagctacaaa tatatgttca gcatgtagta ttttggatgc
     1321 aaaagttgaa aactcatccg cttcaacatg tgacaaagtc atctttaatt catctagaat
     1381 taattgatag tcactaaatt tagccatatt acttaccttc gattgcagcg cgacattgtt
     1441 ttgcagcttc tactggatca tctgcatttg cgattccgcc accaacaata acaagatcag
     1501 gactttcagc gacaatatct ttaattgtat ctggtttaat tccacctgct actgcaactt
     1561 tagaattttt aataacagat ttaacggttc ttaaactttc taatggtgat tgcccttctg
     1621 cttgtaaatc ataaccagtg tgtactgcaa tataatcagc acccatttca tctagttctt
     1681 ttgcacgttt ttctaaatct tgaacagcaa tcatatcaac tagtaattgt ttattatttt
     1741 tatgagcttc ttcaatagct gctttaattg atgcatcttc tgcaacacct agtattgtaa
     1801 ttacatccgc gccaaattta attgcttggc taacttcata atcagctgca tctataattt
     1861 tcatgtctgc taatactttt acattactaa tgttgtctgc catatgttta actgctggta
     1921 aaccttcgtt gtaaatgatt ggcgtaccga tttctacgat atctacataa tcttttactt
     1981 tatttgctaa ctcagccgcg tcttctttgt ttaataaatc aattgctaat tgtaattcca
     2041 ctaaaaatca tccttccaat gctttttatc tttctattgg aacctttccc gaggcatttc
     2101 ttaattaaac attgcaatca atccatttca tcgacatgaa ttttcgcaac atttttcggg
     2161 caagcttcgt cgtctaaata tatctcaaca ttcgggtgtt tgtgaagtaa tgtggctgga
     2221 acatcaacag aaatttcctg atttaataaa tgtgtaatag cagcacgttt cttttcacca
     2281 aatgcgagta aaatgatacg tttggcttga agaatattag caagtcccat cgaaatggct
     2341 tgctttggaa catcatcttc gtttttaaaa tatcgactat tagccttaat agtactttca
     2401 gtcaaatcaa cgatatgagt aacgctatca aacggcgtac caggttcatt aaatccaata
     2461 tgaccatttt caccaatacc taaaatttga atatcacgtt gaccttgttg ttctaaaacg
     2521 tcattatatt ttgacgcttc cgcattcata tcatcggcat ctccatttgg aatatgaatg
     2581 ttctttctat taaaataagg atattgtttg aaaagcatgt catccatata atagtgataa
     2641 ctttgcggat gtgatgcggt taaacctaca tattcgtcta aattaaacgt ggatacattg
     2701 tctacgttta actgattttt atttaataac ttaacaagtt gttcatacaa atctgtcatt
     2761 gtaccaccag ttgctaaacc tagtttacag tgctgattaa atgccatctc tttatataac
     2821 tcacatgcaa catagaatga tgcttgtttt ttcgatccta agtttaatac tttcattgtt
     2881 tacatgcctc cacaaattta atacatttta aaaacttatg aat
//
LOCUS       DALAGD010000176         2894 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.184, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000176 DALAGD010000000
VERSION     DALAGD010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.184"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..>2894
                     /locus_tag="O2701_002735"
     CDS             <1..>2894
                     /locus_tag="O2701_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fibrinogen-binding adhesin SdrG C-terminal
                     domain-containing protein"
                     /protein_id="HCZ4294589.1"
                     /translation="RDNKKAITKKGMISNRLNKFSIRKYTVGTASILVGTTLIFGLGN
                     QEAKAAENTSTENAKQDDATTSDNKEVVSETENNSTTENNSTNPIKKETNTDSQPEAK
                     KESTSSSTQKQQNNVTATTETKPQNIEKENVKPSTDKTATEDTSVILEEKKAPNNTNN
                     DVTTKPSTSEPSTSEIQTKPTTPQESTNIENSQPQPTPSKVDNQVTDATNPKEPVNVS
                     KEELKNNPEKLKELVRNDSNTDHSTKPVATAPTSVAPKRVNAKMRFAVAQPAAVASNN
                     VNDLIKVTKQTIKVGDGKDNVAAAHDGKDIEYDTEFTIDNKVKKGDTMTINYDKNVIP
                     SDLTDKNDPIDITDPSGEVIAKGTFDKATKQITYTFTDYVDKYEDIKSRLTLYSYIDK
                     KTVPNETSLNLTFATAGKETSQNVTVDYQDPMVHGDSNIQSIFTKLDEDKQTIEQQIY
                     VNPLKKSATNTKVDIAGSQVDDYGNIKLGNGSTIIDQNTEIKVYKVNSDQQLPQSNRI
                     YDFSQYEDVTSQFDNKKSFSNNVATLDFGDINSAYIIKVVSKYTPTSDGELDIAQGTS
                     MRTTDKYGYYNYAGYSNFIVTSNDTGGGDGTVKPEEKLYKIGDYVWEDVDKDGVQGTD
                     SKEKPMANVLVTLTYPDGTTKSVRTDANGHYEFGGLKDGETYTVKFETPTGYLPTKVN
                     GTTDGEKDSNGSSVTVKINGKDDMSLDTGFYKEPKYNLGDYVWEDTNKDGIQDANEPG
                     IKDVKVTLKDSTGKVIGTTTTDASGKYKFTDLDNGNYTVEFETPAGYTPTVKNTTADD
                     KDSNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQDSTEKGIKDVTV
                     TLQNEKGEVIGTTKTDENGKYRFDNLDSGKYKVIFEKPAGLTQTVTNTTEDDKDADGG
                     EVDVTITDHDDFTLDNGYFEEDTSDSDSDSDSDSDSDSDSDSDSDSDSDS"
BASE COUNT         1206 a          449 c          512 g          727 t
ORIGIN      
        1 cagggataat aaaaaggcaa taacaaaaaa gggtatgatt tcaaatcgct taaacaaatt
       61 ttcgattaga aagtatactg taggaactgc atcgatttta gtaggtacga cattgatttt
      121 tggtctaggg aaccaagaag ctaaagctgc tgaaaacact agtacagaaa atgcaaaaca
      181 agatgatgca acgactagtg ataataaaga agtagtgtcg gaaactgaaa ataattcgac
      241 aacagaaaat aattcaacaa atccaattaa gaaagaaaca aatactgatt cacaaccaga
      301 agctaaaaaa gaatcaactt catcaagtac tcaaaaacag caaaataacg ttacagctac
      361 aactgaaact aagcctcaaa acattgaaaa agaaaatgtt aaaccttcaa ctgataaaac
      421 tgcgacagaa gatacatctg ttattttaga agagaagaaa gcaccaaata atacaaataa
      481 cgatgtaact acaaaaccat ctacaagtga accatctaca agtgaaattc aaacaaaacc
      541 aactacacct caagaatcta caaatattga aaattcacaa ccgcaaccaa cgccttcaaa
      601 agtagacaat caagttacag atgcaactaa tccaaaagaa ccagtaaatg tgtcaaaaga
      661 agaacttaaa aataatcctg agaaattaaa agaattggtt agaaatgata gcaatacaga
      721 tcattcaact aaaccagttg ctacagctcc aacaagtgtt gcaccaaaac gtgtaaacgc
      781 aaaaatgcgc tttgcagttg cacaaccagc agcagttgct tcaaacaatg taaatgattt
      841 aattaaagtg acgaagcaaa caatcaaagt tggcgatggt aaagataatg tggcagcagc
      901 gcatgacggt aaagatattg aatatgatac agagtttaca attgacaata aagtcaaaaa
      961 aggcgataca atgacgatta attatgataa gaatgtaatt ccttcggatt taacagataa
     1021 aaatgatcct atcgatatta ctgatccatc aggagaggtc attgctaaag gaacatttga
     1081 taaagcaact aagcaaatca catatacatt tacagactat gtagataaat atgaagatat
     1141 aaaatcacgc ttaactctat attcgtatat tgataaaaaa acagttccaa atgagacaag
     1201 tttgaattta acatttgcta cagcaggtaa agaaacaagc caaaatgtca ctgttgatta
     1261 tcaagatcca atggtccatg gtgattcaaa cattcaatct atctttacaa aattagatga
     1321 agataagcaa actattgaac aacaaattta tgttaaccca ttgaaaaaat cagcaaccaa
     1381 cactaaagtt gatatagctg gtagtcaagt agatgattat ggaaatatta aactaggaaa
     1441 tggtagcacc attattgacc aaaatacaga aataaaggtt tataaagtta actctgatca
     1501 acaattgcct caaagtaata gaatctatga ttttagtcaa tacgaagatg taacaagtca
     1561 atttgataat aaaaaatcat ttagtaataa tgtagcaaca ttggattttg gtgatattaa
     1621 ttcagcctat attatcaaag ttgttagtaa atatacacct acatcagatg gcgaactaga
     1681 tattgcccaa ggtactagta tgagaacaac tgataaatat ggttattata attatgcagg
     1741 atattcaaac ttcatcgtaa cttctaatga cactggcggt ggcgacggta ctgttaaacc
     1801 tgaagaaaag ttatacaaaa ttggtgacta tgtatgggaa gacgttgata aagacggtgt
     1861 tcaaggtaca gattcaaaag aaaaaccaat ggcaaacgtt ttagttacat taacttaccc
     1921 ggacggtact acaaaatcag taagaacaga tgctaatggt cattatgaat tcggtggttt
     1981 gaaagacgga gaaacttata cagttaaatt cgaaacgcca actggatatc ttccaacaaa
     2041 agtaaatgga acaactgatg gtgaaaaaga ctcaaatggt agttcggtta ctgttaaaat
     2101 taatggtaaa gatgatatgt ctttagatac tggtttttac aaagaaccta aatacaactt
     2161 aggtgactat gtatgggaag atactaataa agatggtatc caagatgcaa atgagccagg
     2221 aatcaaagat gttaaggtta cattaaaaga tagtactgga aaagttattg gtacaactac
     2281 tactgatgcc tcgggtaaat ataaatttac agatttagat aatggtaact atacagtaga
     2341 atttgaaaca ccagcaggtt acacgccaac ggttaaaaat actacagctg atgataaaga
     2401 ttctaatggt ttaacaacaa caggtgtcat taaagatgca gataatatga cattagacag
     2461 tggtttctat aaaacaccaa aatacagttt aggtgattat gtttggtacg acagtaataa
     2521 agacggcaaa caagattcaa ctgaaaaagg tatcaaagat gtgacagtta cattgcaaaa
     2581 cgaaaaaggc gaagtaattg gaacaactaa aacagatgaa aatggtaaat atcgtttcga
     2641 taatttagat agcggtaaat acaaagttat ttttgaaaag cctgctggct taacacaaac
     2701 agttacaaat acaactgaag atgataaaga tgcagatggt ggcgaagttg acgtaacaat
     2761 tacggatcat gatgatttca cacttgataa cggatacttc gaagaagata catcagacag
     2821 cgattcagac tcagatagtg actcagacag cgactcagac tcagacagcg actcagactc
     2881 agacagtgat tcag
//
LOCUS       DALAGD010000177         2884 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.185, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000177 DALAGD010000000
VERSION     DALAGD010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.185"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..279
                     /locus_tag="O2701_002736"
     CDS             <1..279
                     /locus_tag="O2701_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HCZ4294590.1"
                     /translation="TINSPKLRTQIEDALIKADLNVYDKPTPFLFGEDFSFYGQQLAP
                     AYFVFIGTRNEDKGFVTGLHTSHLNFDEKVLINVVNFYENLLNNYKEV"
     gene            284..1369
                     /locus_tag="O2701_002737"
     CDS             284..1369
                     /locus_tag="O2701_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCZ4294591.1"
                     /translation="MTATWSVNKKIFLQNAITVKNNQPLMAVVKNNAYHYDLEFAVTQ
                     FIHAGIDTFSTTSLREAIQIRQLAPDATIFLMNAVYEFDLVREHQIHMTLPSLTYYYN
                     HKNDLAGIHVHLEFENLLHRSGFKDLNEIKEVLKDHHHNQNAKMIISGLWTHFGYADE
                     FDVSDYNVERSQWMEIVEALLSEGYQFDLIHAQNSASFYREGQILLPHHTHARVGIAL
                     YGSRPYSSLNQHDIVQSLTLKAHVIQVREVQAGDYCGYSFAFEVTKNNTKLAVVDIGY
                     GDGILRTRAKHEALINGKRYPIRALMMSHMFVEVDGNVHAQDEVILYNNDIRIDEYTF
                     KGVGANSEQLSAMNHDSLKKEYISNDC"
     gene            1359..2624
                     /gene="lysA"
                     /locus_tag="O2701_002738"
     CDS             1359..2624
                     /gene="lysA"
                     /locus_tag="O2701_002738"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCZ4294592.1"
                     /translation="MTVKYNQNGELTMDGISLKTIAQSFGTPTIVYDELQIREQMRRY
                     HRAFKDSGLKYNISYASKAFTCIQMVKLVAEEDLQLDVVSEGELYTALEAGFEPSRIH
                     FHGNNKTKHEIRYALENNIGYFVIDSLEEIELIDRYANDTVQVVLRVNPGVEAHTHEF
                     IQTGQEDSKFGLSIQYGLAKKAIDKVQQSKHLKLKGVHCHIGSQIEGTEAFIETAKIV
                     LRWLKEQGIQVELLNLGGGFGIKYVEGDESFPIESGIKDITDAIKSEIKVLGIDAPEI
                     GIEPGRSIVGEAGVTLYEVGTIKEIPEINKYVSIDGGMSDHIRTALYDAKYQALLVNR
                     NEEADDSVTIAGKLCESGDIIIKDAKLPSSVKRGDYLAILSTGAYHYSMASNYNQMQK
                     PSVFFLKDGKAREVIKRQSLRQLIINDTK"
BASE COUNT         1036 a          431 c          516 g          901 t
ORIGIN      
        1 acaatcaata gtccgaaatt acgtactcaa atagaggacg ccttaataaa agctgattta
       61 aatgtctatg acaaaccaac gccattctta tttggggaag attttagttt ttatggtcaa
      121 caactagctc cagcttactt tgtttttata ggaacacgaa atgaagataa aggttttgta
      181 actggtttgc acacatcaca tttaaatttt gatgaaaaag tgttaataaa cgtggttaat
      241 ttttacgaaa atttattaaa taattacaaa gaggtgtaat acattgacag caacatggtc
      301 tgtaaataag aaaatatttt tacaaaatgc aatcacagtc aaaaacaatc agccattaat
      361 ggcagttgtt aaaaataatg catatcacta tgacctagaa tttgctgtaa ctcagtttat
      421 ccatgcaggt atagatacat ttagcacaac atcactacga gaagcaattc aaattagaca
      481 acttgctcca gatgcaacaa tctttttaat gaatgcagtt tacgagtttg atttagtccg
      541 tgaacatcaa atacatatga ctttgccgtc gttgacatat tactataacc ataaaaatga
      601 tttagctggt attcatgttc acttagaatt tgaaaattta ttacatcggt ctggatttaa
      661 agatttaaac gaaattaaag aagtattgaa agatcaccat cataatcaaa atgcaaaaat
      721 gattattagt ggtttatgga cccattttgg atatgctgat gaattcgatg tgtcagatta
      781 taatgttgaa cgttcacaat ggatggaaat tgttgaagca cttttatctg aaggttatca
      841 gttcgaccta atccatgctc aaaatagtgc gagtttttat cgggaaggac aaatattact
      901 accccaccat acacatgcgc gtgtaggtat tgcgttatac ggttcaagac catatagttc
      961 actgaatcaa catgatatag ttcagtcatt aactttaaaa gcacatgtta ttcaagtgcg
     1021 cgaagtacaa gctggtgatt attgcggtta tagctttgcc tttgaagtga ctaaaaacaa
     1081 tacaaaatta gctgtagttg atatcggtta tggcgatgga attttaagaa ctcgtgctaa
     1141 acatgaagca cttatcaatg gtaaacgcta cccgatacgt gcattaatga tgagccatat
     1201 gtttgttgaa gtagatggca atgtacatgc acaagatgaa gttattcttt ataataatga
     1261 tatccgcatc gatgaatata cctttaaagg tgttggtgca aattctgaac aattaagtgc
     1321 tatgaatcat gattctttaa aaaaggagta catttcaaat gactgttaaa tataatcaaa
     1381 atggcgaatt aacaatggat ggtattagtt taaaaacgat tgcacaaagc tttggtacac
     1441 ctaccattgt ttatgatgaa ctacaaatta gagaacagat gcgccgttac catcgcgcat
     1501 ttaaagatag tggattaaaa tacaatattt catacgcctc aaaggcattt acttgcattc
     1561 aaatggtcaa acttgtagct gaggaagatt tacagttaga tgttgtttct gaaggtgaat
     1621 tatatacagc tttagaagca ggttttgaac cgagtcgcat ccatttccat ggtaacaata
     1681 aaacgaaaca tgaaattagg tatgctttag aaaataatat cggttatttt gttatagatt
     1741 cattagaaga aattgaatta atagaccgct atgctaatga tacggttcaa gttgtattac
     1801 gagttaatcc aggtgttgaa gcacatacac acgaatttat tcaaactggg caagaagata
     1861 gtaagtttgg attatcaatt caatatggct tagctaaaaa agcaattgac aaagtccaac
     1921 aatctaaaca cttaaaatta aaaggtgtac attgtcatat tggttcacag attgaaggta
     1981 cagaagcatt tattgaaact gctaaaattg ttttacgttg gcttaaagag caaggcattc
     2041 aagttgaatt attaaacctt ggtggtggct ttggtattaa atatgttgaa ggtgacgaaa
     2101 gtttccctat cgaaagtggt attaaagata ttacagacgc aataaaatcc gaaattaaag
     2161 ttctaggtat agatgcacca gaaataggta ttgaaccggg acgatcaatt gtaggtgaag
     2221 ctggcgttac tttatatgaa gttggaacca ttaaagaaat tccagagatt aataaatatg
     2281 tttcaatcga tggcggtatg agtgatcata tcagaactgc actttatgac gcaaagtatc
     2341 aagcattgct tgttaataga aatgaagaag cagatgacag tgtaactata gctggaaaat
     2401 tatgtgagtc tggtgatatc attattaaag acgctaaatt accttcatca gtcaaacgtg
     2461 gagactatct tgctatatta tcaactggtg catatcatta ctctatggca tccaattaca
     2521 atcaaatgca aaagccttct gtgtttttct taaaagatgg caaagcacgt gaagttataa
     2581 agcgacaatc gttaagacaa ctcattatta atgatacaaa ataaaaataa acaaagtaat
     2641 ccccgaagca cagaaaatta tactgtgact cggggaattt ttatgcatta tagatgactg
     2701 tagaaaacat gcttggacat atactctaaa aaaggaagaa cagcacattt ttagttagaa
     2761 agtatttatt tctttttgtc attaatcctt tatatttagt tagccctatt caactaattt
     2821 aaagcatcat atacatacca cactttaaac aagcatcatg aaattagatt ttatgcttta
     2881 cttg
//
LOCUS       DALAGD010000178         2854 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.186, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000178 DALAGD010000000
VERSION     DALAGD010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.186"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..385
                     /gene="lpl4"
                     /locus_tag="O2701_002739"
     CDS             <1..385
                     /gene="lpl4"
                     /locus_tag="O2701_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl4"
                     /protein_id="HCZ4294593.1"
                     /translation="KIILLDKVEDKKLKQKIENFKFFSQYANLKELKNYSNGDVSINE
                     NVPSYDVKYKMSNKDENVKQLRSRYNIPTDKSPVLKMHIDGNLKGSSVGDRKLEIDFS
                     KRENSHLSVIDSLDYQPAKTNKDDE"
     gene            404..1174
                     /gene="lpl5"
                     /locus_tag="O2701_002740"
     CDS             404..1174
                     /gene="lpl5"
                     /locus_tag="O2701_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl5"
                     /protein_id="HCZ4294594.1"
                     /translation="MKSIKRIGLCISLLILSIFVTSCDGDNKITGDSKETQIKKSFSK
                     TLDMYPIKNLEDLYDKEGYRDGEFKKGDKGMWTIYTDFAKGNKSDELDDEGMVLNLDR
                     NTRTAKGYYFVKKFYEKDKLPDRKNYKVEMKNNKIILLDKVEDTNLKKRIENFKFFGQ
                     YANFKDLENYNNGDVSINWNVPSYDVEYKMSNKDENVKQLRSRYNIPTDKAPMLKMHI
                     DGDLKGSSVGYKRLEIDFSKEGRDISVIDYLSYKPAKK"
     gene            1206..1997
                     /gene="lpl6"
                     /locus_tag="O2701_002741"
     CDS             1206..1997
                     /gene="lpl6"
                     /locus_tag="O2701_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl6"
                     /protein_id="HCZ4294595.1"
                     /translation="MEYLKRLALLISVIILTIFIMGCDSQSDTAENPKEGSKEAQIKK
                     SFSKTLDMYPIKNLEDFYGKEGYRDGEFKKDDKGTWLIRSEIVKQPKGKVMKTRGMQL
                     YINRNTETAKGFFVLKEISENNNRVNKDKEEKYEVKMVGNKIIPTEQINDEKIKKEIE
                     NFKFFVQYGNFKNFEKYNNGEFSYNPEAPIYSAKYQLHNDDYNVRQLRKRYDISTKET
                     PKLLLKGGGDLKNSSVGQNDIEFTFVERKGENIYFNDSVEFIPSK"
     gene            2067..2843
                     /gene="lpl7"
                     /locus_tag="O2701_002742"
     CDS             2067..2843
                     /gene="lpl7"
                     /locus_tag="O2701_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl7"
                     /protein_id="HCZ4294596.1"
                     /translation="MKCFQKLYIFILILIVLMAGCESNKITGDSKETQIKKSFAKTLD
                     VYPTKNLEDFYDKEGYRDGEFKKGDKGKWVIRSEMTTELKNENMVSKGMVIRLNRNSR
                     TCTGEYFVRIVKEDSEGKVYSDERKYPVKMENNKIITLKPIDDEKVKKEIEEFKFFVQ
                     YGNFKELENYKDGEVTYNPEAPIYSAQYQLKNSDYNVEQLRKRYNITTKKAPKLLLKG
                     SGNLKGSSVGYKNIEFTFVENKEENIYFTDSINFNPSEDK"
BASE COUNT         1221 a          289 c          540 g          804 t
ORIGIN      
        1 taaaatcatt ttattagata aagtagaaga taaaaagctt aaacaaaaaa tagaaaattt
       61 taaatttttc agtcaatatg caaatcttaa agaattgaaa aactacagca atggtgatgt
      121 ctcaattaat gagaatgttc caagttatga cgtgaaatat aaaatgagta ataaagatga
      181 aaatgttaag caattaagaa gtcgttataa tattcctact gataaatcac cggtattaaa
      241 aatgcatatt gatggtaatt tgaaaggaag ttccgttgga gataggaagt tagaaattga
      301 tttttcgaaa cgtgaaaata gccatttatc agtaatagat tctttggatt accagccagc
      361 gaaaactaat aaagatgatg aataatgagg atggtgtgta acaatgaagt ctataaaaag
      421 gattggattg tgcataagtc tgttgatttt aagcattttt gtaacatctt gcgatggtga
      481 taataagatc actggagatt caaaagaaac acaaatcaaa aagagctttt cgaaaacgtt
      541 agatatgtat ccaattaaga atctcgagga cttatatgat aaagaaggat accgtgatgg
      601 cgaatttaaa aagggcgaca aaggaatgtg gactatatat acagattttg ctaaaggcaa
      661 taaatcagac gaattggatg atgaaggtat ggttttaaat ctggatagaa atactcgaac
      721 ggctaaggga tattattttg ttaagaaatt ttatgaaaag gataaattac ctgatagaaa
      781 aaattataaa gttgaaatga aaaataataa aattatctta ttagataaag tagaagatac
      841 aaatctaaaa aagagaatag aaaactttaa atttttcgga caatatgcaa attttaagga
      901 tttggaaaat tacaacaatg gcgacgtgtc aataaattgg aatgttccaa gttatgacgt
      961 ggaatataaa atgagcaata aagatgaaaa tgttaaacaa ttaagaagtc gttataacat
     1021 tcctactgat aaagctccaa tgttaaaaat gcatattgac ggggacttaa aaggtagttc
     1081 tgttggatat aaaaggttag aaatagattt ttcaaaagaa ggtagggata tttcagtcat
     1141 tgattattta agttataagc cagcgaaaaa atagtactga taattaaata ttagggtgtg
     1201 aaggaatgga atatctaaaa aggcttgcat tgttaataag tgttattatt ttgaccattt
     1261 ttataatggg ttgtgatagt caaagcgata ctgcagaaaa tccaaaagaa ggttcaaaag
     1321 aagcacaaat taaaaagagt ttttcgaaaa cgttagatat gtatccaatt aagaatctcg
     1381 aggattttta tggcaaagaa ggatatcgag atggcgaatt taaaaaagat gataaaggta
     1441 cttggctaat tagatccgaa atagttaaac agccaaaggg caaagtgatg aaaacaagag
     1501 gtatgcaatt atatattaat agaaataccg aaacagccaa aggtttcttt gttttgaaag
     1561 aaataagtga aaataataat cgtgtaaata aagataagga ggaaaaatac gaagtgaaaa
     1621 tggtaggaaa taaaattatt cctactgaac aaattaatga cgagaaaata aaaaaagaaa
     1681 ttgaaaactt caagtttttt gtgcaatatg gaaactttaa aaatttcgaa aaatacaaca
     1741 atggtgagtt ttcatataat cctgaagcac caatttattc ggctaaatat caattacaca
     1801 acgatgatta caatgtaagg caactacgta aaagatatga catttcaaca aaagaaacac
     1861 cgaagttact tttgaaaggt ggaggagatt taaaaaattc ctcagttggt caaaacgata
     1921 ttgaatttac ttttgttgaa agaaaaggtg agaatattta ttttaacgat agtgttgaat
     1981 tcataccaag taagtaaagt taaattaaaa tgtgtggtta gatgaaatga tcaaatatca
     2041 aaaaatgtgc aaagagatgt gacaagatga agtgttttca gaaattatac atatttatat
     2101 taattttaat cgtattaatg gcaggatgcg aaagtaataa gatcactgga gattcgaaag
     2161 aaacacagat caaaaagagc tttgcgaaaa cattagatgt ataccctacg aaaaatctag
     2221 aagattttta tgacaaagaa ggatatcgag atggcgaatt taaaaagggt gacaaaggga
     2281 agtgggttat tagatctgaa atgacaacag aactgaaaaa tgaaaatatg gtatctaaag
     2341 gtatggtcat acgtttaaat agaaatagta gaacatgcac tggtgaatat tttgtcagga
     2401 tagttaaaga agacagtgag ggcaaggtat atagtgatga acgaaaatat ccagtgaaaa
     2461 tggaaaataa taaaatcatt acattaaaac caatcgatga tgaaaaagta aaaaaagaaa
     2521 ttgaagaatt taaattcttc gtacaatacg ggaatttcaa agaattggaa aactataaag
     2581 acggagaagt gacatataac ccagaagcac caatatactc tgcacaatat caattgaaaa
     2641 atagtgatta taatgtagaa caactacgta agcgatataa tataacgacg aaaaaagcgc
     2701 ctaaattatt attgaagggt tcaggtaatt taaaaggctc atcagttgga tataaaaata
     2761 ttgaatttac ctttgttgaa aataaggaag aaaatattta cttcacagat agtattaatt
     2821 tcaacccaag tgaggataaa taagtatatt tagt
//
LOCUS       DALAGD010000179         2715 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.187, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000179 DALAGD010000000
VERSION     DALAGD010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.187"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            76..516
                     /locus_tag="O2701_002743"
     CDS             76..516
                     /locus_tag="O2701_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HCZ4294597.1"
                     /translation="MLSQEFFNSFITIYRPYLKLAEPILEKHNIYYGQWLILRDIAKH
                     QPTTLIEISHRRAIEKPTARKTLKALIENDLITVENSLEDKRQKFLTLTPKGHELYEI
                     VCLDVQKLQQAVVAKTNISQDQMQETINVMNQIHEILLREAHND"
     gene            509..1720
                     /locus_tag="O2701_002744"
     CDS             509..1720
                     /locus_tag="O2701_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ4294598.1"
                     /translation="MTNQGSLNKSPIFTKSFTINFLVNFIVYLCMYLLLVVIAGYSKQ
                     AFDASDSLAGLVVGLFIVGSLIGRFATGKFVNQIGPKRLLFIGLIALIITQLLYFIDG
                     SLAFLIFVRLINGIATAVVTTATGTIAAYVTPVNRKSEGISLFSLSLVLGTAIGPFLG
                     MLLITKYAIDLLFIICVILGILGLIISLFIKVDFEVTNNKTETNVIDKPRFSIHQFIA
                     KEAIPVAVIMLLIGVTYASILTYLQAFALERHLVTAASYFFICYAIASLITRPIAGRL
                     MDDKNENIIVYPAFIMLFLSFVCLISSYQSWLILLAGACLGLGYGNLSSAMQSIAIKV
                     SPPIKYGIATSTFYVGLDAGVGFGPSFLGLFTHMFSYSEIFGFMAALAIITMLVYFLI
                     HGRHVTRNAMN"
     gene            complement(1822..2172)
                     /gene="sarV"
                     /locus_tag="O2701_002745"
     CDS             complement(1822..2172)
                     /gene="sarV"
                     /locus_tag="O2701_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarV"
                     /protein_id="HCZ4294599.1"
                     /translation="MSNKVQRFIEAERELSQLKHWLKTTHKISIEEFVVLFKVYEAEK
                     ISGKELRDTLHFEMLWDTSKIDVIIRKIYKKELISKLRSETDERQVFYFYSTSQKKLL
                     DKITKEIEVLSVTN"
     gene            2298..2468
                     /locus_tag="O2701_002746"
     CDS             2298..2468
                     /locus_tag="O2701_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294600.1"
                     /translation="MFRFISFGQLVVYSTTFIPKKDILQLNGMECDINVKEPLKIYYV
                     VILKSGIEMIFS"
BASE COUNT          819 a          482 c          415 g          999 t
ORIGIN      
        1 ttgctcccgt tttataatta ctattgttgt aaaaaggtta gctaagctaa ctattttgtc
       61 ttaggagatg tcactatgct atcacaagaa tttttcaata gttttataac aatatatcgc
      121 ccctatttaa aattagccga gccgatttta gaaaaacaca atatatatta tggccaatgg
      181 ttaatcttac gcgatatcgc taaacatcag cccactactc tcattgaaat ttcacataga
      241 cgtgcaattg aaaagcctac tgcaagaaaa actttaaaag ctctaataga aaatgacctt
      301 ataacagtag aaaacagttt agaggataaa cgacaaaagt ttttaacttt aacacctaaa
      361 gggcatgaat tatatgagat tgtttgtctt gatgtacaaa agctccaaca agcagtagtt
      421 gccaaaacaa acatttcgca agatcaaatg caagaaacca taaatgtgat gaatcaaatt
      481 catgaaatat tattaaggga ggcacacaat gactaatcaa ggctcattaa ataaatcacc
      541 tattttcacc aaaagtttta caattaactt tttagtcaac tttatcgtat acctatgcat
      601 gtatttgtta cttgtagtta ttgctggcta tagtaagcaa gctttcgacg cttccgattc
      661 attagccgga ttagttgtag gactatttat tgtcgggtct ttaattggtc gatttgctac
      721 aggtaaattt gtaaatcaaa tcggacctaa acgattatta tttattggtt taattgcttt
      781 aattatcacg caattacttt attttattga tggatcttta gcttttttaa tctttgtacg
      841 tctcataaac ggtatcgcaa ctgcagttgt gacaactgca acaggtacta ttgcggcata
      901 cgttacccct gttaatagaa aaagtgaagg catcagttta ttttccttaa gtttagtatt
      961 aggtacagcg attggcccat ttctaggcat gttacttatt acaaaatacg caattgactt
     1021 actgtttatt atttgtgtca tattaggcat actcggactc attatctcat tatttataaa
     1081 agttgatttc gaagtaacaa ataacaaaac tgaaactaat gtcattgata aaccaagatt
     1141 tagtattcat caatttattg caaaagaggc cattcctgtt gctgtaatta tgcttttaat
     1201 tggcgttacc tatgcgtcca tcctaactta tttacaagca tttgctttag agcgacacct
     1261 agtcactgca gcaagctatt tctttatttg ctatgctata gcatcgttaa ttactagacc
     1321 tattgctggt agattgatgg atgataaaaa tgaaaatatt attgtctacc cagcatttat
     1381 catgcttttc ctttcatttg tatgcttaat ttcaagctat caaagctggt taatattact
     1441 tgctggtgct tgcctaggtt tagggtatgg aaatttatca tctgcaatgc aatctattgc
     1501 gataaaagtc tcacccccga ttaaatatgg tatcgcgaca tctacttttt acgttggtct
     1561 agatgcaggt gtcggcttcg gtccatcatt ccttgggtta tttactcata tgttttcata
     1621 tagtgaaatc ttcggcttta tggccgcttt agcaatcatt actatgcttg tttacttctt
     1681 aattcatgga cgtcatgtaa ctagaaatgc catgaactaa atatttatat tcaaaaataa
     1741 aaagcatgcc aatctctatt catcatttgt ctgtccccaa gacgcaatta tgatgtactg
     1801 attggcatgc tttttaagtt tttagtttgt aacgcttaac acttctattt ctttagtaat
     1861 tttatctaac aatttctttt gagaagtact atagaaatag aatacttgtc tttcatccgt
     1921 ttcagaacgc aatttagaaa taagctcttt tttatagatt ttacggataa tcacatcgat
     1981 tttacttgta tcccatagca tttcaaaatg taatgtatcc ctcaattctt taccgctaat
     2041 cttttcagct tcatacactt taaaaaggac tacaaattct tcaattgaaa tcttatgtgt
     2101 tgtttttaac cagtgcttta actgacttaa ctccctttct gcttctataa aacgttgaac
     2161 tttattactc ataatatgat gcctcctatg tttcttctta gatgtttgta attcatatta
     2221 ttatacaatt acttaaattt attatatatc aaacttagca ttttatctat tacaaatatt
     2281 aaattgctgg ctatatcttg tttagattta tatcttttgg acaacttgtt gtttattcga
     2341 caacatttat acctaaaaaa gatatattac aactcaatgg catggagtgt gacatcaatg
     2401 ttaaagaacc actaaagatt tattatgtag tgattcttaa gagtgggata gaaatgatat
     2461 tctcataaaa tttatttcgt tgatccccaa cttgcattgt ctgtagaatt tcttttgaaa
     2521 ttctctatgt tggggccccg ccaacttgca cattattgta tgctgacttt tcgtcacctt
     2581 ctgtgttggg gccccgttcc ccaacttgca ttgtctgtag aatttctttt tgaaattctc
     2641 tatgttgggg ccccgccaac ttgcacatta ttgtaagctg actttctgtc agcttctgtg
     2701 ttggggcccc gccaa
//
LOCUS       DALAGD010000180         2639 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.188, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000180 DALAGD010000000
VERSION     DALAGD010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.188"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            1312..2181
                     /locus_tag="O2701_002747"
     CDS             1312..2181
                     /locus_tag="O2701_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HCZ4294601.1"
                     /translation="MEMSRLNLTDDNDIDEIIAWRGEQEELEQKRDTYKKRYHEFEME
                     IARLESLTKDKELLDSDKLKDEYELKKGKMNTLIDEYSAVHYQCQNNINKTQSIVSHI
                     NYLNQELKDQQEIFQLAEIVSGKNNKNLTLENFVLIYYLDQIIAQANLRLATMSDNRY
                     QLIRREAVSHGLSGLEIDVFDLHSNKSRHISSLSGGETFQSSLALALGLSEIVQQQSG
                     GISLESIFIDEGFGTLDQETLETALDTLLNLKSTGRMVGIISHVSELKNRIPLVLEVK
                     SDQYQSSTRFKRN"
     gene            complement(2252..2614)
                     /gene="mscL"
                     /locus_tag="O2701_002748"
     CDS             complement(2252..2614)
                     /gene="mscL"
                     /locus_tag="O2701_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCZ4294602.1"
                     /translation="MLKEFKEFALKGNVLDLAIAVVMGAAFNKIISSLVENIIMPLIG
                     KIFGSVDFAKEWSFWGIKYGLFIQSVIDFIIIAFALFIFVKIANTLMKKEEAEEEAVV
                     EENVVLLTEIRDLLREKK"
BASE COUNT         1100 a          329 c          423 g          787 t
ORIGIN      
        1 aacaaaaata aaattgattt cgttcaattg aaagaacaac aacctgaaat agagggaatt
       61 gaagctaagt taaagttgtt acaagatatt acaaacctat tgaattatat tgaaaataga
      121 gaaaaaattg aaactaaaat tgctaatagc aaaaaagata tttctaaaac caataataaa
      181 atattgaatc ttgattgtga taagcgaaac atagacaaag agaaaaaaat gttagaagaa
      241 aatggagatt taattgaaag taaaatctct tttattgata aaactagagt attatttaac
      301 gatattaata agtatcaaca aagttatctc aatattgaac gcttgagaac tgagggtgaa
      361 caattaggtg atgaattaaa tgatctaatt aaaggtttag aaacggtcga agattcaata
      421 ggtaataacc aaagtgatta cgagaaaatt atcgaactaa ataatacgat aacgaacata
      481 aataatgaaa ttaatataat taaagaaaat gaaaaagcta aagctgaatt agataaacta
      541 ttaggtagta agcaagagtt agagaatcaa attaatgaag aaacatctat attgaagaat
      601 ctcgaaataa aattagatcg ctacgataaa acaaaattgg acttaaatga taaagaaagc
      661 tttataagtg aaattaaatc tgctgtaaat attggagatc aatgtccgat atgtggtaat
      721 gaaattcagg atttagggca tcatattgat tttgacagta ttgctaaacg tcaaaatgaa
      781 attaaagaaa ttgaagcaaa tatccacgct ataaaatcga atattgctgt gcataattct
      841 gaaattaaat ttgttaatga aaaaatatcg aatattaata ttaaaacgca aagtgatttt
      901 tcacttgaag tattgaataa gcgtctgcta gaaaatgaaa atgcattgaa taatcaaaga
      961 gatcttaata aatttataga acaaatgaaa gaagaaaaag ataatctaac gttgcaaatt
     1021 cataataaac aattgcgtct aaataaaaat gaatctgagt tgaaattatg tcgagatctc
     1081 atcactgaat ttgaaacact ctcgaaatat aataatatca ctaattttga ggtggattat
     1141 aagaagtatg ttcaagatgt gaatcagcat caagaactct caaaggagat tgaagataag
     1201 ttaatgcaat tgtctcaaag aaagttaatt gagcaaaata atctaaatca ttatgaaaat
     1261 caactagaaa cttacaataa tgacttagaa ttgaatgaac agtctattga aatggaaatg
     1321 tcgaggctga atttaactga tgacaatgat atagatgaaa taatagcctg gaggggcgag
     1381 caagaggaat tagagcagaa aagggatact tataaaaaac gttatcatga atttgaaatg
     1441 gaaatagcta ggttagaatc attaactaag gataaagagt tattggactc tgataaatta
     1501 aaagatgaat atgagctaaa aaaaggaaag atgaatacac tgatagatga atactctgct
     1561 gttcattatc aatgtcaaaa taatattaat aaaacacaat ctatagtttc gcatattaat
     1621 tacttaaatc aagaattaaa ggatcaacaa gaaatatttc aattggctga aattgtcagt
     1681 ggtaagaata acaaaaatct tacattggaa aactttgtct taatttacta tttagatcaa
     1741 attattgctc aagcaaatct gagattagca acaatgtcag ataatcgata ccaactaatt
     1801 aggcgagaag cggtttctca tggtcttagt ggcctagaaa ttgatgtatt tgatttgcat
     1861 tcaaataagt ctagacatat tagctcgtta tcaggtggag aaactttcca atcgtcgctt
     1921 gcattagctt tagggttaag cgaaattgta cagcagcaat caggaggtat ttcactagaa
     1981 tcaatattta ttgatgaagg attcggtaca ttagatcaag aaacgcttga aacagcgtta
     2041 gacactttat taaatcttaa atcaactggt agaatggttg ggattatttc acatgtgagc
     2101 gaattgaaaa atagaatacc tttagtttta gaagtgaaat cagatcaata tcagagttca
     2161 acaagattca aaagaaatta aaacataaaa aatagagatg gtactttgga atgtatagaa
     2221 caaccttagc ccatctcttt ttatttaaaa attatttttt ctcacgtaat aaatctctga
     2281 tttcagttaa taacacaaca ttttcttcca caactgcttc ttcttcggct tcttctttct
     2341 tcattaatgt atttgcaatc ttaacaaaga tgaataaagc aaacgcgatg ataataaagt
     2401 cgataacaga ttggataaat aaaccgtatt taatacccca gaatgaccat tctttagcaa
     2461 aatcaactga tccgaaaatt ttaccaatta atggcataat gatattttct actaatgaag
     2521 atataatctt gttgaaagct gcacccatca caacagcaat tgctaaatct aagacgttac
     2581 cttttaaggc gaactctttg aattctttta acattacact caacctctct tttttaaat
//
LOCUS       DALAGD010000181         2616 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.189, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000181 DALAGD010000000
VERSION     DALAGD010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.189"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            120..1289
                     /locus_tag="O2701_002749"
     CDS             120..1289
                     /locus_tag="O2701_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NarK/NasA family nitrate transporter"
                     /protein_id="HCZ4294603.1"
                     /translation="MYKTKGGFQLTLQTLSLVVGFMAWSIIAPLMPFIKQDVNVTEGQ
                     ISIILAIPVILGSVLRVPFGYLTNIVGAKWVFFTSFIVLLFPIFFLSQAQTPGMLMAS
                     GFFLGVGGAIFSVGVTSVPKYFPKEKVGLANGIYGMGNIGTAVSSFLAPPIAGIIGWQ
                     TTVRSYLIIIALFALIMFIFGDTQERKIKVPLMAQMKTLSKNYKLYYLSYWYFITFGA
                     FVAFGIFLPNYLVNHFGIDKVDAGIRSGVFIALATFLRPIGGILGDKFNAVKVLMIDF
                     VVMIIGAIILGISDHIALFTVGCLTISICAGIGNGLIFKLVPSYFLNEAGSANGIVSM
                     MGGLGGFFPPLVITYVANLTGSSHLAFIFLAVFGCIALFTMRHLYQKEYGSLKNG"
     gene            complement(1396..1824)
                     /locus_tag="O2701_002750"
     CDS             complement(1396..1824)
                     /locus_tag="O2701_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HCZ4294604.1"
                     /translation="MILNFNQFENQNFFNANPSDTFKDLGKQVFNYFSTPSFVTNIYE
                     TDELYYLEAELAGVNKEDISIDFNNNTLTIQATRSAKYKSEQLILDERNFESLMRQFD
                     FEAVDKQHITASFENGLLTITLPKIKPSNETTSSTSIPIS"
     gene            complement(2040..2486)
                     /locus_tag="O2701_002751"
     CDS             complement(2040..2486)
                     /locus_tag="O2701_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCZ4294605.1"
                     /translation="MYVENSYLSKQLCFLFYVSSKEIIKKYTNYLKEYDLTYTGYIVL
                     MAIENDEKLNIKKLGERVFLDSGTLTPLLKKLEKKDYVVRTREEKDERNLQISLTEQG
                     KAIKSPLAEISVKVFNEFNISEREASDIINNLRNFVSKNFDYSDKK"
BASE COUNT          843 a          366 c          441 g          966 t
ORIGIN      
        1 tgtctatatt gaatgttttc atataaatga aagcattttt aaataacatt gacctctaat
       61 atataggcag agtattgata tctattaaaa aataaatgat tttgatgaag gtgaaacgta
      121 tgtacaaaac aaaaggtggc tttcaactta cattacaaac attaagttta gtggttgggt
      181 ttatggcttg gagtataatt gcgccattaa tgccctttat taaacaagat gtcaatgtta
      241 ctgaaggtca aatatcaatc attttagcga taccagttat tttgggatcg gtgctccgtg
      301 tgccatttgg ttatttaaca aacattgttg gcgctaaatg ggtattcttt actagtttta
      361 tcgtattgtt attcccgata tttttcttaa gccaagcaca aacaccgggt atgttaatgg
      421 cttcaggatt tttccttggt gtaggtggtg caattttctc agttggtgtt acatcagttc
      481 ctaaatattt ccctaaagaa aaagtaggtc tagcaaatgg tatttatggt atgggaaata
      541 tcggtacagc agtttcttca tttttagcac caccgatagc gggtattatt ggttggcaaa
      601 caacagttag aagttactta attattatcg ctttatttgc attaattatg ttcatttttg
      661 gtgacacaca agaacgtaaa attaaagtac cattaatggc tcaaatgaaa acattatcta
      721 aaaactacaa attatattac ttaagttatt ggtattttat tacttttggt gcttttgtag
      781 catttggtat tttcttacct aactacttag taaatcattt tggaattgac aaagtagatg
      841 ctggtattcg atcaggtgta ttcattgcgc tggcaacatt cttaagacca ataggtggca
      901 ttttaggtga taaatttaat gcagttaaag tattgatgat tgattttgtt gttatgatta
      961 tcggtgccat tattttaggt atttcagacc atatcgcatt attcactgta ggctgtttaa
     1021 caataagtat ttgtgcaggt attggtaacg gcttaatctt caaattagta ccatcatact
     1081 tcttaaatga agcgggatcc gcaaatggta tcgtatcaat gatgggtggt ttaggaggat
     1141 tcttcccacc actagtaatc acgtacgtag ctaatttaac aggatcaagt catttagcat
     1201 ttattttctt agcggtattt ggatgtattg cattatttac catgcgtcat ttataccaaa
     1261 aagaatatgg ctcattgaaa aacggttgat atgtaataca tgccattcat ttagttaaat
     1321 acaaagcctt aatatcatgc gcaatattcg tagcatgaca ttaaggcttt agtagtgtat
     1381 tcattttaaa tttaactatg aaattggaat agatgttgat gaagtagttt cattgcttgg
     1441 tttgatttta ggcaaggtaa tggttaataa cccattttca aaactagcag taatatgttg
     1501 cttatcaaca gcttcaaaat caaattgacg cattaatgat tcgaagttac gctcatctaa
     1561 aatgagttgt tcagatttgt attttgcgct tctagtagct tgaatagtga gcgtattatt
     1621 attgaaatcg atactaatat cttctttatt tacacctgct agttcagctt ctaagtaata
     1681 taattcgtct gtttcatata tattcgttac aaatgaaggt gttgaaaagt aattaaatac
     1741 ttgtttacct aaatctttaa atgtatcact tggattagcg ttaaaaaagt tttgattctc
     1801 gaattgattg aagttcaata tcatcacttc cattatagaa atttgtatta caaagccatt
     1861 atatgagtag tattaaaaaa gttaaagtaa aaggggagat aaaaaagtaa taaatgggta
     1921 gatttgtgcc tgataaggaa tcagggcaaa agaactgagc atcgagattt atacaagcaa
     1981 taaagataat gaataaacat atcgatgcat acttctgccc atcaccttat gatagggagt
     2041 tattttttgt cggaatagtc aaaattttta gaaacaaaat tccgcaaatt attaattata
     2101 tctgatgctt ctcgttctga aatgttgaat tcattaaaga ccttaacaga aatttcagca
     2161 agagggcttt ttattgcttt accttgttct gttagagaaa tttgtaggtt tctttcatct
     2221 ttctcttcac gtgttcgaac aacgtaatct ttcttttcta atttctttag taatggtgtc
     2281 agtgttccag aatctaagaa cacacgttca cctaattttt tgatgttaag tttttcatca
     2341 ttttcaatcg ccattaaaac aatgtaacca gtataggtta aatcatattc cttaagatag
     2401 tttgtgtatt ttttaattat ttcttttgaa gaaacataaa ataaaaaaca caactgtttg
     2461 ctaagatagc tgttttctac atacatccaa tcactccttg ttaaaataaa caatattatg
     2521 aagtgtaata gttgtcaatt actgatttca tcgcaattat tgaattgtct ataattgaat
     2581 ttaaatgtta aataatgcaa aatatatcga tatctt
//
LOCUS       DALAGD010000182         2520 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.190, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000182 DALAGD010000000
VERSION     DALAGD010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.190"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(230..517)
                     /locus_tag="O2701_002752"
     CDS             complement(230..517)
                     /locus_tag="O2701_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294606.1"
                     /translation="MTNEDKRFEQLRFERKFIVIPYLIYAVIVLLLNIFYSDLKITMT
                     LFGLFFAYNVVILFIAFVKHYKRTLLLSLILTVLSGAAFFGIIYVYGINHF"
     gene            complement(552..1460)
                     /locus_tag="O2701_002753"
     CDS             complement(552..1460)
                     /locus_tag="O2701_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCZ4294607.1"
                     /translation="MEQIMINHYVHFSRLVQGFWRANEWKMTAKELNYFINELVERGI
                     TTMDHADIYGDYQCESLFGNALDLSPELRNKIQIVTKCGIILPSKQFDFTNGHRYDLS
                     SKHIVKSVEQSLINLNVDYLDSLLIHRPSPLMDPEQVADALTKLVKQGKLKSFGVSNF
                     NHSQYQLLNQYIMKERLHISINQLELSPYHVDSLQDGTMDSMYQNHVQIMAWSPFAGG
                     KIFDKEDIKAQRIMKVVQSIADKYGVSDTAVMIAWLVKIPHRIMPILGTSQLKRIDQA
                     IEGLQLNLDDQSWFDIYTAIIVQDIP"
     gene            complement(1563..2489)
                     /locus_tag="O2701_002754"
     CDS             complement(1563..2489)
                     /locus_tag="O2701_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCZ4294608.1"
                     /translation="MKNNKDEKIRISIINGFLGSGKTTLLTHYISELLKNDEKIKIIM
                     NEFGTFDIDSNSISNEIEVHSLINGCVCCDLKQELVYELKAIALKGDVNHVIIEATGI
                     AHPLELLVACQDPQIVNFFEKPIIYGVLDATRFLERHQYTENTVSLMEDQLKLSDMII
                     INKIDLITDDSLEKIDKQLGMICASIPTYKTTYGKVSLEELDLTVKDREISSHHHHHH
                     GIKSMTYTFTGPIDRHLFYQFIMKLPESVLRLKGYVSFRDQPNAIYEFQYAYGLPDYG
                     IIGMQLPLTIVIIGETLDTNHIRNQLDMLQFT"
BASE COUNT          864 a          433 c          335 g          888 t
ORIGIN      
        1 tacattttta atttatgcaa aatacacaat tttatcgaaa tattttgtaa ataatattgc
       61 taggtgatga gttatctcgg ttaattacaa aacgatatct aagtttaaat attagatcat
      121 aaaaaaacta tctactgaat atcctcatag catcattttt ctaagcaaaa gttaaactta
      181 gttagataaa atgcaaatga gattattgta gatagtctct ttttaatgtt taaaaatgat
      241 taatgccata aacataaata attccaaaga atgccgcgcc actaagcact gttaatataa
      301 gacttagtaa caatgtgcgt ttataatgtt taacaaatgc tatgaacaaa atgactacat
      361 tatacgcaaa gaaaagtccg aataatgtca ttgttatttt caaatcagaa tagaaaatat
      421 ttaatagtaa tacaatgact gcataaatta aatacggaat aactataaat ttgcgttcaa
      481 atcttaattg ttcgaaacgt ttatcttcgt ttgtcatgat atgctccttt atgatttaaa
      541 agtaaataag tttacggaat atcttgtacg ataatagcgg tgtaaatgtc aaaccacgac
      601 tgatcatcta aattaagttg tagcccttcg attgcttgat caatacgctt taactgactt
      661 gttccaagta tcggcatgat acgatgcggt atttttacta accacgctat catcacagct
      721 gtgtcactca caccatattt gtcagctatt gattgaacaa ctttcataat acgttgcgct
      781 ttaatatctt ccttgtcgaa aattttaccg cctgcaaaag gactccaagc catgatttga
      841 acatggtttt gatacattga atccattgtt ccatcttgta aactatcaac gtgatatggc
      901 gataattcta attgattgat gctaatatgt agtctttctt tcataatata ttgatttaac
      961 aattggtatt gtgaatgatt aaaattcgac accccgaatg acttcaactt accttgttta
     1021 acaagtttag ttaatgcatc agcaacttgt tctggatcca tcaatggtga aggacgatga
     1081 atgagtagac tatctaaata atctacattc aaattgatta atgactgttc aacagatttc
     1141 acgatgtgct tactactcaa atcataacga tgtccatttg taaaatcaaa ttgcttagaa
     1201 ggcaaaatga taccacattt cgtaacaatt tgaattttat ttcttaattc gggtgataaa
     1261 tccaaagcat taccaaacag tgattcacat tgataatctc cataaatatc agcatgatcc
     1321 atcgttgtaa ttccacgttc aactaattca tttataaaat aatttaactc tttcgcagtc
     1381 atcttccatt catttgcacg ccaaaaacct tgtacaagcc tagaaaaatg aacatagtga
     1441 ttaatcatta tttgttccat atctcatcca ctccatccct caatgtcaca aaatattcac
     1501 aataaaagta ttaagaaatt aatgtgctat ttccgttaaa ttgaaacaac acattttctg
     1561 atttacgtaa attgtagcat atccaattga ttacgtatgt gatttgtatc taaagtttca
     1621 ccaataataa caatcgttaa tggtaattgc atgccaatta ttccatagtc tggtaaacca
     1681 tatgcatatt gaaattcata aattgcattt ggttgatctc taaatgacac ataacctttc
     1741 aaacgtagaa cagattccgg taatttcatt ataaattgat aaaacaaatg acgatcaatc
     1801 ggacctgtaa acgtgtaagt catactttta atcccatgat gatggtgatg atgagacgat
     1861 atctctctgt ctttaacagt taagtccaat tcttccaacg aaacttttcc ataggttgtt
     1921 ttataagttg gaatacttgc acaaatcata cctaattgct tatcaatttt ctcaagactg
     1981 tcatcagtta taagatcaat tttattaata ataatcatgt cacttagttt caactgatct
     2041 tccatcagcg aaactgtatt ttcggtatat tgatgacgtt ctaaaaatcg agtcgcatct
     2101 aatacaccat aaataatcgg cttttcaaag aaattaacga tttgcggatc ttgacatgca
     2161 actagtaatt ccaaaggatg cgcaatgcct gtcgcttcta tgatgacatg attaacgtcc
     2221 ccttttaaag caatggcttt tagttcatag acaagttctt gtttaagatc gcaacaaaca
     2281 caaccattaa tcaatgaatg gacttcaatt tcatttgaaa tactattgct atcaatatca
     2341 aaagtaccga attcattcat gatgatttta attttctcat cattttttaa taattcacta
     2401 atataatgtg tcagtaacgt ggttttacca ctacccaaaa atccgttaat tatggatatt
     2461 cttatttttt catctttatt atttttcatt ttatattctc ttttctatta cagttatttt
//
LOCUS       DALAGD010000183         2386 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.191, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000183 DALAGD010000000
VERSION     DALAGD010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.191"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            241..2283
                     /gene="lip1"
                     /locus_tag="O2701_002755"
     CDS             241..2283
                     /gene="lip1"
                     /locus_tag="O2701_002755"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip1"
                     /protein_id="HCZ4294609.1"
                     /translation="MKSQNKYSIRKFSVGASSILIATLLFLSGGQAQAAEKQVNMGNS
                     QEDTVTAQSIGDQQTRENANYQRENGVDEQQHTENLTKNLHNDKTISEENHRKTDDLN
                     KDQLKDDKKSSLNNKNIQRDTTKNNNANPSDVNQGLEQAINDGKQSKVASQQQSKEAD
                     NSQDSNANNNLPSQSRIKEAPSLNKLDQTSQREIVNETEIEKVQPQQNNQANDKITNY
                     NFNNEQEVKPQKDEKTLSVSDLKNNQKSPVEPTKDNDKKNGLNLLKSSAVATLPNKGT
                     KELTAKAKDDQTNKVAKQGQYKNQDPIVLVHGFNGFTDDINPSVLAHYWGGNKMNIRQ
                     DLEENGYKAYEASISAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKTYEGI
                     YKDWKPGQKVHLVGHSMGGQTIRQLEELLRNGNREEIEYQKKHGGEISPLFKGNHDNM
                     ISSITTLGTPHNGTHASDLAGNEALVRQIVFDIGKMFGNKNSRVDFGLAQWGLKQKPN
                     ESYIDYVKRVKQSNLWKSKDNGFYDLTREGATDLNRKTSLNPNIVYKTYTGEATHKAL
                     NSDRQKADLNMFFPFVITGNLIGKATEKEWRENDGLVSVISSQHPFNQAYTKATDKIQ
                     KGIWQVTPTKHDWDHVDFVGQDSSDTVRTREELQDFWHHLADDLVKTEKLTDTKQA"
BASE COUNT          972 a          346 c          440 g          628 t
ORIGIN      
        1 gactaattaa attttgccaa atataatatt aattaataat ttgaaatgat tagcgtatac
       61 actttaaatt ctctttggag aatatatttt ttaaatacaa atgtaaacgc tttctcgtca
      121 aattaaacaa tagaaaggat ggtcattatg agtgcttggt taagtaaatt atttgagttt
      181 attcctcgaa taattatcaa tttgtttatc taaaataaaa aaatagaggt gctgacaatg
      241 atgaaaagtc aaaataagta tagtattcgt aaatttagtg taggtgcatc ttccatttta
      301 atagctacat tactattttt aagtggtgga caagcacaag cagctgagaa gcaagtgaat
      361 atgggaaatt cacaggagga tacagttaca gcacaatcta ttggggatca acaaactagg
      421 gaaaatgcta attatcaacg tgaaaacggt gttgacgaac agcaacatac tgaaaattta
      481 actaagaact tgcataatga taaaacaata tcagaagaaa atcatcgtaa aacagatgat
      541 ttgaataaag atcaactaaa ggatgataaa aaatcatcgc ttaataataa aaatattcaa
      601 cgtgatacaa caaaaaataa caatgctaat cctagcgatg taaatcaagg gttagaacag
      661 gctattaatg atggtaaaca aagtaaagtg gcgtcacagc aacagtcaaa agaggcagat
      721 aatagtcaag attcaaacgc taataacaat ctaccttcac aaagtcgaat aaaggaagca
      781 ccatcattaa ataagttaga tcaaacaagt caacgagaaa ttgttaatga gacagaaata
      841 gagaaagtac aaccacaaca aaataatcaa gcgaatgata aaattactaa ctacaatttt
      901 aacaatgaac aagaagtgaa acctcaaaaa gacgaaaaaa cactatcagt ttcagattta
      961 aaaaacaatc aaaaatcacc agtagaacca acaaaggaca atgacaagaa aaatggatta
     1021 aatttattaa aaagtagtgc agtagcaacg ttaccaaaca aagggacaaa ggaacttact
     1081 gcaaaagcga aagatgatca aacgaataaa gttgccaaac aagggcagta taaaaatcag
     1141 gatcctatcg ttttagtgca tggtttcaat gggtttacag atgatattaa tccttcagtg
     1201 ttagctcatt attggggcgg taataaaatg aacattcgcc aagatttaga agaaaatggt
     1261 tacaaagctt atgaagcaag tataagtgct tttggaagta actatgaccg cgcagttgaa
     1321 ctttattatt atatcaaagg cggtcgtgta gattatggtg cagcacatgc agcaaaatat
     1381 ggacatgaac gttatggaaa aacatacgaa ggaatttaca aagactggaa accaggacag
     1441 aaggtacacc tagttggaca tagtatgggc ggtcaaacga tacgtcaact agaagaatta
     1501 ctgcgtaatg gtaatcgtga agaaatagag tatcaaaaga aacatggtgg cgaaatttct
     1561 ccactattca aaggtaatca tgacaatatg atttcatcaa ttactacttt aggaacacca
     1621 cataatggta cacacgcatc agatttagct ggtaatgaag ctttagtgag acaaatcgta
     1681 tttgatatcg gtaaaatgtt tggtaataaa aattcaagag tagacttcgg gttggctcaa
     1741 tggggtctaa aacagaagcc aaatgaatca tatattgatt atgtcaaacg cgttaaacaa
     1801 tctaatttat ggaaatcaaa agataatgga ttttacgatc tgacgcgtga gggtgcaaca
     1861 gatttaaatc gtaaaacgtc gttgaaccct aacattgtgt ataaaacata cactggtgaa
     1921 gcaacgcaca aagcattaaa tagcgataga caaaaagcag acttaaatat gtttttccca
     1981 tttgtgatta ctggtaactt aatcggtaaa gctactgaaa aagaatggcg agaaaacgat
     2041 ggtttagtat ccgttatttc ttctcaacat ccatttaatc aagcttatac aaaagcgaca
     2101 gataaaattc aaaaaggcat ttggcaagtg acgcctacaa aacatgattg ggatcatgtt
     2161 gactttgtag gacaagacag ttctgataca gtgcgcacaa gagaagaatt acaagatttt
     2221 tggcatcatt tagcagacga tttagtgaaa actgaaaagc tgactgatac taagcaagca
     2281 taatttataa agtaaaggga ggaattaata atgactgcag acttctttca attaatcgga
     2341 tcattattta gaattctaaa agaattattc aagtaaaaca ttggcg
//
LOCUS       DALAGD010000184         2376 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.192, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000184 DALAGD010000000
VERSION     DALAGD010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.192"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            289..603
                     /locus_tag="O2701_002756"
     CDS             289..603
                     /locus_tag="O2701_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCZ4294610.1"
                     /translation="MTYKELATFLKVLSDSSRLEILDLLSCGELCACDLLAHFQFSQP
                     TLSYHMKALVKTNLVTTRKIGNKHLYQLNHNIFESVINNLSKVHTSNQRCICHNLKTG
                     EC"
     gene            603..1895
                     /gene="arsB"
                     /locus_tag="O2701_002757"
     CDS             603..1895
                     /gene="arsB"
                     /locus_tag="O2701_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter membrane subunit
                     ArsB"
                     /protein_id="HCZ4294611.1"
                     /translation="MMTTLATLIFLVTLLFVLWQPKGLDIGITALTGAFIAVITGVVS
                     FSDVFEVTGIVWNATLTFVSVILISLILDKVGLFEWSAIHMLHASKGNGLKMFVYIIL
                     LGAIVAAFFANDGAALILTPIVLAMVKNIGFSKRAIFPFIIASGFIADTTSLPLIVSN
                     LVNIISADYFHVGFVRYFSRMIIPNLFSLLASIIVLWLYFRKAIPKTFDDNNIKHPKD
                     AINDLKLFKISWIVLVILLFGYLISEFTKIPVSIFTGIIAFIFLMLARKSNAVNIKQV
                     IKGAPWNIVLFSIGMYIVVFGLRNAGITLILAKILEYISNYGLFSTILGMGFISAFLS
                     SIMNNMPTVLIDAIAIGQSNVHGMLKEGLIYANVIGSDLGPKITPIGSLATLLWLHVL
                     TQKDVKISWGTYFKTGIIITIPVLFITLIGLYLTLIIF"
     gene            1913..2308
                     /gene="arsC"
                     /locus_tag="O2701_002758"
     CDS             1913..2308
                     /gene="arsC"
                     /locus_tag="O2701_002758"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HCZ4294612.1"
                     /translation="MTKKTIYFICTGNSCRSQMAEGWAKQILADDWNVYSAGIETHGV
                     NPKAIEAMKEVGIDISNHTSDLIDNNIIKNSNLVVTLCSDADVNCPSLPTNVKKEHWG
                     FDDPAGKPWSEFQRVRDEIKIAIENFKSR"
BASE COUNT          780 a          358 c          389 g          849 t
ORIGIN      
        1 ctatttatga tttttagtcg taaaaacaaa gcttcttaat caataccctt gattacaaaa
       61 taaattcaac taaggtgatt ggccatacac tcaagatatg gctaatcacc tttttcaatg
      121 cagaacaaag agtatatttt gcaaaatcaa tatctataat ttatctttaa taccctttta
      181 atactaaaac caaatttaaa ttacatagtc atatcgtgta atataccaaa ggaacataga
      241 cattcatcta tatagatgat aatctattta acgtctagga ggtatgttat gacgtataaa
      301 gaactagcaa catttttaaa agttttatca gattcaagca gattagaaat actagattta
      361 ctttcttgtg gagagttatg cgcttgtgat ttgttagcac attttcaatt ctctcaacct
      421 acacttagct atcatatgaa agcattagta aaaaccaact tagttacgac acgaaaaatc
      481 ggaaataaac atttatacca gctcaatcat aatatttttg agtccgtaat taataacttg
      541 tctaaggttc atacctctaa ccaacgatgt atttgtcata accttaagac tggtgaatgc
      601 taatgatgac aactttagcg acactcattt ttctagtaac tttattattt gtattatggc
      661 aacctaaagg cctagatatt ggcattaccg cactaactgg tgcctttatt gctgttatta
      721 ctggtgttgt aagtttttcc gatgttttcg aagtaacagg tattgtttgg aatgctactt
      781 tgacttttgt ctcagtcatt cttatttcat taatattaga taaagttgga ttattcgaat
      841 ggtcagctat tcacatgctt catgcttcaa aaggcaatgg tttaaaaatg ttcgtttata
      901 tcatattatt gggtgccatt gttgctgcat ttttcgcaaa tgatggcgca gcgttaatct
      961 taacgcctat tgtattagcg atggttaaaa atataggttt tagtaagcgg gccatattcc
     1021 cttttattat tgcgagtggt tttatagctg acacaacttc tttaccttta atcgtgagca
     1081 atctagtgaa tattatatct gctgattatt ttcatgtagg attcgttcga tattttagta
     1141 gaatgattat acctaattta ttctcgcttt tagcaagtat tatagtattg tggttatatt
     1201 ttagaaaagc gatacctaaa acgtttgatg ataataatat aaagcatcct aaagatgcca
     1261 ttaatgattt aaagctattt aaaatttcat ggattgttct agttatatta cttttcggct
     1321 atctaatcag tgaatttact aaaattccgg tatcaatttt cactggaatc attgctttta
     1381 tttttctaat gttggctcgt aaatcaaatg ctgtaaatat taagcaagtc attaagggcg
     1441 caccttggaa tatagtatta ttttcaattg gtatgtatat cgtcgtattc ggcttaagaa
     1501 atgctggcat tactttaata ttggctaaaa tattagaata tatttccaat tacggtctat
     1561 ttagcactat tttgggaatg ggcttcattt cagcgttttt atcatcaata atgaataata
     1621 tgcctacagt tttaatagat gcgattgcta ttggtcaatc aaatgtccat ggcatgttaa
     1681 aagaaggcct aatttatgcg aatgttatcg gttctgattt aggtccaaaa attacaccga
     1741 taggctcttt agctacatta ctgtggttac acgtcttaac acaaaaagat gttaagattt
     1801 cttggggcac atactttaaa actggtatca tcattacaat tccagtacta tttataaccc
     1861 tcatagggtt gtatctaaca cttatcatat tttaattaat taggagattg ttatgactaa
     1921 aaaaacaatt tattttatat gtacaggcaa ctcatgtcga agtcaaatgg ctgaaggttg
     1981 ggctaaacaa atcttagcgg atgattggaa tgtatattct gctggtatcg aaacacacgg
     2041 tgttaatccc aaagcgatag aagctatgaa agaagtaggc attgatatat caaatcatac
     2101 atcagattta atcgataata atattattaa aaattcaaat ttagttgtta cattatgtag
     2161 tgatgcagac gtaaattgcc cttctttacc aacaaatgtt aagaaagaac attggggatt
     2221 tgatgatcct gcaggcaagc cttggtcaga gttccaacgt gtaagagatg aaattaaaat
     2281 cgcaattgaa aatttcaaat cacgatgaga gtgggacaga aatgataaaa aaacactaat
     2341 gatttattat gtagtggttc tttatcatta gctata
//
LOCUS       DALAGD010000185         2355 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.193, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000185 DALAGD010000000
VERSION     DALAGD010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.193"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            308..682
                     /locus_tag="O2701_002759"
     CDS             308..682
                     /locus_tag="O2701_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HCZ4294613.1"
                     /translation="MKRILVVFLMLAIILAGCSNKGEKYQKDIDKVYKEQNQMNKIAS
                     KVQNTIKTDIKQEDSNTHVYKDGKVIVIGIQLYKDREKMYYFAYEIKDGKAEINREID
                     PIKYMKDHKADYEDENVEVEKD"
     gene            835..1233
                     /locus_tag="O2701_002760"
     CDS             835..1233
                     /locus_tag="O2701_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCZ4294614.1"
                     /translation="MEKSIKIMTIIGIVVQGLATVFSLLLMVLAASGVMTTDVSTTVN
                     GEVDPVDAETAAAIFTVLFLSLFIFGIISIILGAIGMFKASKNKKMSGILLIIGAVIS
                     GNIITFALWLISGIKLLTNNKPKDEISDLS"
     gene            complement(1449..2273)
                     /locus_tag="O2701_002761"
     CDS             complement(1449..2273)
                     /locus_tag="O2701_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCZ4294615.1"
                     /translation="MKEKHIKWDKIFYGDDWVNNVVETIRTKDILQSVYLKRYLLRAM
                     MAGFIIGIITVFVLSVKATHEPDLPPGIVNMASAITFSFALVLILFTNSELLTSNFMY
                     FTVGLYYKVIKPTRVLKIFLLCFAGNILGAAILFSFMRFSNVMTPDMLNQLSAVIEHK
                     TLSTGFVSILMKAIFANFFINISLVIAMQIDDVLAKMFVMMFGVTIFAFMGYEHVVYN
                     SCLFMGGLIYQVDTLHFIPAISNIAAAFIGNYIGGGLIIGLFYAYLNDHHQFYKNN"
BASE COUNT          917 a          321 c          373 g          744 t
ORIGIN      
        1 ttgggaatta aataaatata taggaggcaa tttttagaga tacatagcaa tgtaaaggta
       61 tcaaatagtt atttcataat ttattatgat gacataattt tcagtagata gttgtaaatt
      121 aacactgaaa tagatgttta tattaagaag actttaatat cataattaat caaaatcttg
      181 cgtcaaacat ttaaacgaat tttaagttga taacaattgg caaaacattg ttcaaacatc
      241 acaatgataa agcatattat cagtattgta gtgtgtggaa aatgacagcc atctaaggag
      301 aaaaatgatg aaaagaatat tggtagtatt tttaatgtta gcaattatat tggcaggttg
      361 ttctaataaa ggtgaaaagt atcaaaaaga tattgataaa gtgtacaaag aacagaatca
      421 aatgaataaa attgcctcga aagtacaaaa cactattaaa acagacatta aacaagaaga
      481 cagtaataca catgtttata aagatggtaa agtcattgtt attggtattc aattatataa
      541 agatcgtgaa aaaatgtatt atttcgcata tgaaataaaa gatggtaagg cagagattaa
      601 tagagaaata gacccaatta agtatatgaa agaccataaa gcagattatg aagatgaaaa
      661 tgtagaagtg gaaaaagatt aacataggct ttctttaaaa cttttaacca gtttcaaaaa
      721 gtaaatgagc tttagcgaca ataattttta aaataaacac gatataaatt taaacattta
      781 tatataagaa cacaaacgca agtttgtaaa taataataat aaaggagtaa gacaatggaa
      841 aaatcgatca aaataatgac aataatagga attgttgttc agggtttagc aacggtattt
      901 agtttactat tgatggtttt agcagcatca ggtgtaatga ctacagatgt gtcaacaaca
      961 gttaatggtg aggttgaccc agttgatgca gaaacagcag cagcaatttt cactgtatta
     1021 ttcttatccc tattcatatt tggaatcatt tcaattattt taggtgcaat cggtatgttt
     1081 aaagcatcta aaaacaaaaa aatgagtggt atattgttga ttattggagc tgtaataagt
     1141 ggtaacataa ttacatttgc tttatggtta atcagtggta ttaaactact tactaataac
     1201 aagcctaaag atgaaataag cgacttatca taaacatcgt atattgaaat ttcaagattt
     1261 cttaagtaat taaataaagt gctctcttag aagtagattt tcagtcaata actgctttta
     1321 agagagcatt ttgtatttga taatgctcat agcatcggat aaaaatcatt gacgaacttt
     1381 aaaaaaatac gttagacaat gcacttctaa atattaaagg catcgactaa cgcattgttc
     1441 aaatatagtt aattattttt ataaaattga tgatgatcat tcaagtaagc atagaataaa
     1501 cctataatga gtccgcctcc aatatagtta ccgataaaag ccgcagcgat attcgaaata
     1561 gctggtatga agtgcaatgt atcaacttga taaattaaac cacccataaa taagcaactg
     1621 ttgtaaacga cgtgttcata acccataaag gcgaatatgg taacaccgaa catcatgaca
     1681 aacatttttg cgagtacatc gtcaatttgc atggcaataa ctaatgaaat attgataaag
     1741 aaattggcga agatcgcttt cattaatata cttacaaaac cagtagacaa cgttttatgc
     1801 tctataactg ctgataactg atttaacata tctggcgtca ttacatttga aaaacgcatg
     1861 aaactaaata aaatagcagc acctaaaata tttcctgcaa agcataataa aaatattttc
     1921 aatactctag ttggtttaat tactttataa tacaggccta cagtaaagta catgaagtta
     1981 ctggttagta gttcggagtt tgtaaataaa atgagtacta acgcaaagct gaatgtaatg
     2041 gcactggcca tattcacaat gcctggcggt aaatctggtt cgtgtgttgc tttaactgat
     2101 aatacgaaga ccgtaataat cccgataata aatcctgcca tcatcgcgcg taataaataa
     2161 cgttttaaat aaacgctttg taatatatct tttgttctta tcgtttcgac tacgttattt
     2221 acccagtcgt ccccataaaa aatcttatcc catttaatat gtttctcctt cacaatgaca
     2281 tcctcacttt tcatttgtta atcagagtat actatgaatt gccttatttg tgaataattt
     2341 cacaaaatta atttt
//
LOCUS       DALAGD010000186         2158 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.194, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000186 DALAGD010000000
VERSION     DALAGD010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.194"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..227
                     /gene="sarT"
                     /locus_tag="O2701_002762"
     CDS             <1..227
                     /gene="sarT"
                     /locus_tag="O2701_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarT"
                     /protein_id="HCZ4294616.1"
                     /translation="VFKNDEGKYFMRDIISYIGIDQSRIVKSVKDLSKKGYLNKCRDP
                     HDSRNVIIVVSVKQHNYIKNLLSEININET"
     gene            533..2158
                     /gene="sasG"
                     /locus_tag="O2701_002763"
                     /pseudo
     CDS             533..2158
                     /gene="sasG"
                     /locus_tag="O2701_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored surface protein SasG"
BASE COUNT          876 a          272 c          419 g          591 t
ORIGIN      
        1 atgtatttaa aaatgatgaa ggtaaatatt ttatgagaga cattataagt tatatcggta
       61 tagaccaatc tagaattgtt aaaagcgtaa aagatttatc aaaaaagggt tacttgaata
      121 aatgtagaga cccacatgat agtagaaatg taatcattgt tgtttctgta aaacaacaca
      181 attatattaa aaatcttttg agtgaaataa atattaatga aacataaagt atgtttcgag
      241 attttaatat cttggtgcag ttaaatgtat tttttaaatg cgttaaggta ttttttattt
      301 tagaaattca atgctattga gttgatgggt ttttccaaca gataagaatg aattattcat
      361 tgaaaactaa aaataataat tttatttgta gattgtttta agtgcgtcaa tatataaaat
      421 aattttataa agataagtgt tatattccat tgtgcaactc ctattgtatt tgtaaaatta
      481 catgtaaatt cactgtaagc aaagtggaaa atatggaaaa aggagtatgc aaatgagaga
      541 taagaaagga ccggtaaata aaagagtaga ttttctatca aataaattga ataaatattc
      601 aataagaaaa tttacagttg gaacagcatc tattttaatt ggctcactaa tgtatttggg
      661 aactcaacaa gaggcagaag cagctgaaaa caatattgag aatccaacta cattaaaaga
      721 taatgtccaa tcaaaagaag tgaagattga agaagtaaca aacaaagaca ctgcaccaca
      781 gggtgtagaa gctaaatctg aagtaacttc aaacaaagac acaatcgaac atgaaccatc
      841 agtaaaagct gaagatatat caaaaaagga ggatacacca aaagaagtag ctgatgttgc
      901 tgaagttcag ccgaaatcgt cagtcactca taacgcagag acacctaagg ttagaaaagc
      961 tcgttctgtt gatgaaggct cttttgatat tacaagagat tctaaaaatg tagttgaatc
     1021 taccccaatt acaattcaag gtaaagaaca ttttgaaggt tacggaagtg ttgatataca
     1081 aaaaaaacca acagatttag gggtatcaga ggtaaccagg tttaatgttg gtaatgaaag
     1141 taatggtttg ataggagctt tacaattaaa aaataaaata gattttagta aggatttcaa
     1201 ttttaaagtt agagtggcaa ataaccatca atcaaatacc acaggtgctg atggttgggg
     1261 gttcttattt agtaaaggaa atgcagaaga atatttaact aatggtggaa tccttgggga
     1321 taaaggtctg gtaaattcag gcggatttaa aattgatact ggatacattt atacaagttc
     1381 catggacaaa actgaaaagc aagctggaca aggttataga ggatacggag cttttgtgaa
     1441 aaatgacagt tctggtaatt cacaaatggt tggagaaaat attgataaat caaaaactaa
     1501 ttttttaaac tatgcggaca attcaactaa tacatcagat ggaaagtttc atgggcaacg
     1561 tttaaatgat gtcatcttaa cttatgttgc ttcaactggt aaaatgagag cagaatatgc
     1621 tggtaaaact tgggagactt caataacaga tttaggttta tctaaaaatc aggcatataa
     1681 tttcttaatt acatctagtc aaagatgggg ccttaatcaa gggataaatg caaatggctg
     1741 gatgagaact gacttgaaag gttcagagtt tacttttaca ccagaagcgc caaaaacaat
     1801 aacagaatta gaaaaaaagt tgaagagatt ccattcaaga aagaacgtaa atttaatccg
     1861 gatttagcac cagggacaga aaaagtaaca agagaaggac aaaaaggtga gaagacaata
     1921 acgacaccaa cactaaaaaa tccattaact ggagtaatta ttagtaaagg tgaaccaaaa
     1981 gaagagatta caaaagatcc gattaatgaa ttaacagaat acggacctga aacaatagcg
     2041 ccaggtcatc gagacgaatt tgatccgaag ttaccaacag gagagaaaga ggaagttcca
     2101 ggtaaaccag gaattaagaa tccagaaaca ggagacgtag ttagaccgcc ggtcgata
//
LOCUS       DALAGD010000187         2140 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.195, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000187 DALAGD010000000
VERSION     DALAGD010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.195"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            61..1059
                     /gene="argF"
                     /locus_tag="O2701_002764"
     CDS             61..1059
                     /gene="argF"
                     /locus_tag="O2701_002764"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCZ4294617.1"
                     /translation="MKNLRNRNFLTLLDFTQKEMEFLLNLSEDLKRAKYAGIEQQKMK
                     GKNIALLFEKDSTRTRCAFETAAYDQGAHVTYLGPTGSQMGKKESTKDTARVLGGMYD
                     GIEYRGFSQRVVEDLAKYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKPYNEINFTY
                     VGDGRNNVANALMQGAAIMGMTFHLVCPKELNPTDELLNRCNDIADKNGGEILITDDI
                     DEGVKGSDVIYTDVWVSMGEPDEVWEKRIKLLEPYRVTKELMKKTGNPHTIFEHCLPS
                     FHDTETIIGKQIQEKYGLTEMEVTNEVFESEQSVVFQEAENRAHTIKAVMVATLGE"
     gene            1079..2008
                     /gene="arcC"
                     /locus_tag="O2701_002765"
     CDS             1079..2008
                     /gene="arcC"
                     /locus_tag="O2701_002765"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCZ4294618.1"
                     /translation="MSKIVVALGGNALGQSPKEQLDLLKSTSKSLVSLIDKGYEIVIS
                     HGNGPQVGSINLGLNYAAEHKQGPPFPFPECGAMSQAYIGYQMQESLQNELHSMGIDK
                     QVVTLVTQVQVASDDSAFNNPTKPIGLFYTKEQADKFTKEKGYTFVEDSGRGYRRVVP
                     SPQPISIVELDSIETLITHGTLVIAAGGGGIPVIKENEVYTGVDAVIDKDKTSALLAA
                     HLQSDQLIILTAVDHVYINYGKENQRGLDEVSVDEMKKHISDGQFAKGSMLPKVEAAL
                     QFLEKNTKGSVLITSLAGLGDALDGKIGTLIKN"
BASE COUNT          803 a          316 c          408 g          613 t
ORIGIN      
        1 gaaattggat tataaaaaaa taacatgaat aaaatattaa ttgaaaagga gatattcaaa
       61 atgaaaaatt taagaaatag aaacttttta actttgttag acttcacaca aaaagaaatg
      121 gaatttttac ttaatttatc tgaagatctt aaacgcgcaa aatatgcagg aatagaacaa
      181 caaaaaatga aaggtaaaaa tatcgctcta ctttttgaaa aagattcaac acgcactcga
      241 tgtgcatttg aaacagcggc ttatgatcaa ggtgcacatg taacatacct tgggccaaca
      301 ggttctcaaa tgggtaaaaa agagtctacc aaagatactg ctcgtgtttt aggtggaatg
      361 tatgatggca tagaatatcg tggattctca caaagagtag ttgaggattt ggcaaaatat
      421 tctggcgttc ccgtatggaa tggtttaaca gatgaagatc atcctacaca agtacttgct
      481 gattttttaa cagctaaaga agtattgaaa aaaccatata atgaaattaa cttcacatat
      541 gttggagatg gacgtaataa cgttgcaaat gccttaatgc aaggagcagc aatcatgggc
      601 atgactttcc atcttgtatg tcctaaagaa ctaaatccaa ctgatgaatt attaaatcgg
      661 tgtaatgata tagctgacaa aaacggtggt gaaatcctta taactgatga tattgatgaa
      721 ggtgtcaaag gatctgatgt aatttacaca gatgtttggg tatctatggg tgaacctgat
      781 gaagtttggg aaaaacgtat caaattatta gaaccatacc gtgtaactaa agaattaatg
      841 aaaaaaacag gtaatccaca tacaattttt gaacattgct taccatcatt ccatgatact
      901 gaaacaataa tcggtaaaca aattcaagaa aaatatggtc taactgaaat ggaagtaaca
      961 aatgaagtat ttgaaagtga acaatcagtt gttttccaag aagcagaaaa tagagcacat
     1021 acaataaaag cagtcatggt agcaacatta ggagagtaaa tgaaaggagg atataaatat
     1081 gtctaaaatc gtcgtagctt tgggtggtaa cgctttagga caatcgccta aagaacaatt
     1141 agatttatta aaaagtacat ctaaatctct agtaagttta atcgataaag gatacgaaat
     1201 tgtaattagt catggtaatg gtccacaagt tggaagtatt aacttaggtt taaattatgc
     1261 ggcggaacac aaacaaggtc ctccttttcc tttccctgaa tgtggtgcta tgagtcaagc
     1321 ttatattggc tatcagatgc aagaaagttt acaaaacgaa cttcattcaa tgggcataga
     1381 taaacaagtt gttacgctag taacacaagt tcaagttgca agcgatgatt ctgctttcaa
     1441 taaccctact aaaccaattg gattatttta tacaaaagag caggctgata aatttacgaa
     1501 agaaaaaggt tatacttttg tagaagattc tggacgcggc tatcgtcgcg ttgtcccttc
     1561 cccacaacct ataagtatag ttgagttaga tagtatagaa acattaatca ctcatggaac
     1621 actagttatc gctgccggcg gtggtggaat tccagtaatt aaggaaaatg aagtttatac
     1681 aggtgttgat gcagttattg ataaagataa aacgagtgct ttattagcag cacatttaca
     1741 atctgatcaa ttaatcatat taactgctgt ggaccatgtt tacattaact atggaaaaga
     1801 aaaccaaaga ggtctcgatg aagtgtctgt ggatgaaatg aaaaaacata tctctgatgg
     1861 tcaatttgct aaaggaagta tgcttccaaa agttgaagct gcacttcaat ttcttgaaaa
     1921 aaatactaaa ggcagtgttt tgattacatc tctagcagga ttaggggacg ctttagacgg
     1981 taaaatagga acattaatta agaattgaaa ttataatact ttagttcaat tacctttata
     2041 aacaaaagag cctataatcc tagggcactg aacccttaaa acgggaactt aataaaaaca
     2101 ccatgttcta ggctattaat tctctgtatt ttacagggga
//
LOCUS       DALAGD010000188         2083 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.196, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000188 DALAGD010000000
VERSION     DALAGD010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.196"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(287..1933)
                     /locus_tag="O2701_002766"
     CDS             complement(287..1933)
                     /locus_tag="O2701_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCZ4294619.1"
                     /translation="MNSSSPENPNGKKYSPVFIYSAIVVAIVVLLGAFLPEQFNYVTN
                     NIKMWITEKLGWYYLILTTIIVFFCIFLIFSPIGKLKLGKPNDKPEFNTISWFAMLFS
                     AGMGIGLVFYGAAEPMAHFATPPTADPKTTEAYTEALRSTFFHWGFHAWAVYGVVALA
                     LAYSQFRKGEPGLLSRTLRPLLGDKVEGPIGIFIDVLSVFATIVGVAVSLGMGALQIN
                     GGLHYLFNVPNNTFVQAIIIIVVTILFIASAWSGLSKGIQYLSNLNIGLGTILMVAAL
                     IVGPTVLILNMLTSSTGSLLNTFLFNSFDTAALNPQKREWMSSWTLYYWGWWLSWSPF
                     VGVFIARVSKGRSIREFISGVLLVPAIVSFVWFSVFGVLGIETGKKHKEIFDMTPETQ
                     LFGVFNHVPFGIVLSLIALLLIASFFITSADSATFVLGMQTTFGSLNPSSMVKVVWGI
                     SQALIAFVLLLAGGGNGAEALNAIQSAAIISAFPFSFVVILMMVSFYKDANQERKFLG
                     LTLTPNKHRLQEYIKSQQEDYESDILEKRQSRRNIEKKDN"
BASE COUNT          750 a          401 c          298 g          634 t
ORIGIN      
        1 attgatttat gtcccagcct gagttaattt tctataaaag tatatttaat ttgcgtttat
       61 accgtcaaac ttcactttag ctttgtcaaa cccctttcta ttaagttttc agaaataaac
      121 ctatcttaaa atataaaaaa atcgagaatt cgtagtttaa taacgaaatt ctcgttctta
      181 tccttttgaa tatactcaat tttccacaaa aacaaacaag tagtatatct gttctagcta
      241 ctagaatgac atactacttg ttattaaaat acttaactaa actttattag ttatcttttt
      301 tctctatatt tctacgtgac tgacgctttt caagaatgtc agattcataa tcttcttgtt
      361 gactcttgat atattcttgt aagcgatgtt tattcggagt caatgttaaa cctaggaatt
      421 tacgttcctg gttcgcatcc ttgtagaaac ttaccatcat gagtatgacg acaaaggaga
      481 atgggaatgc acttataatt gcagcacttt gaatcgcatt taaagcttca gcgccgttac
      541 cgccaccagc taataaaagt acaaatgcta ttaaggcctg tgaaattccc caaacaactt
      601 ttaccatact agatggattt aatgaaccaa atgttgtttg cattcctaat acaaatgttg
      661 ctgagtcagc agatgtaata aagaatgatg caattaataa taatgcaatc aacgataaaa
      721 caatgccaaa tggcacatga ttaaacactc caaatagctg tgtttcagga gtcatatcaa
      781 aaatttcttt gtgtttctta cctgtctcga tgcctaatac accaaagaca ctaaaccaaa
      841 caaaactaac aattgctgga actagcaaga caccagaaat gaactctcta attgaacgtc
      901 cttttgaaac tcgtgcaata aacactccaa cgaatggact ccaacttaac caccaacccc
      961 agtaataaag tgtccatgaa gacatccatt cacgtttttg aggatttaaa gctgctgtat
     1021 caaaactatt aaacaagaat gtgtttagta aactacccgt agagctagtt aacatattta
     1081 aaataagaac agttggtcca acaattaaag cagctaccat taaaatagta cctaaaccaa
     1141 tgttcaagtt acttaagtat tgaatacctt tacttaatcc agaccatgca cttgctataa
     1201 ataagatagt aacaacaatg atgataatcg cttgtacaaa cgtattgttt ggaacattga
     1261 acaagtaatg taaaccacca ttaatttgta gagcacccat acctaacgaa acggctaccc
     1321 caacgattgt cgcaaataca gataaaacgt caataaaaat cccaatagga ccttctactt
     1381 tatcacctaa aagaggacgt aaagttctag ataataaacc tggttcacct ttacggaatt
     1441 gcgaatatgc caacgctaac gcaacaacac cataaacagc ccaagcatgg aatccccaat
     1501 ggaaaaatgt tgaacgtaga gcttcagtat aagcttcagt agttttggga tctgctgtag
     1561 gtggcgtagc aaagtgcgcc atcggttcag ctgcaccata aaacaccaaa cctatcccca
     1621 taccagcact aaacaacata gcaaaccatg aaattgtatt aaactcaggt ttgtcatttg
     1681 gtttacctag tttaagtttt ccaataggac taaaaataag gaatatacag aagaacacga
     1741 taatcgtagt aagaataaga taataccaac ctaacttttc tgtaatccac attttaatat
     1801 tattggtaac atagttgaat tgttcaggta aaaatgcacc aagtaatacg actatagcaa
     1861 caacaattgc actatagatg aagactggtg aatacttctt tccatttgga ttctctggtg
     1921 aagaagaatt cataattaat tactcccttc aattctatat ttaattttat gtagtagaat
     1981 aaaaatatta tctaaacatt ttattcaata actcacgcac ctttatagga taacaaaggt
     2041 ctttgcgata atcaaatttt cttgaataat ctattttcta att
//
LOCUS       DALAGD010000189         2010 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.197, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000189 DALAGD010000000
VERSION     DALAGD010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.197"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            213..905
                     /locus_tag="O2701_002767"
     CDS             213..905
                     /locus_tag="O2701_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294620.1"
                     /translation="MKIIKLFSIISLFLLVFSANFSNAYANEEQKSSLLENQKEKLKS
                     KNIEVSQMTKKEISFLEQLQNKNEDMQKFEKQMKKEGFKKIKLEDKNSKLKYLATEAN
                     DKNAYGYIANNAYKNEKTKEIVVSETIYDNYNKKITKFIAEKRSMTENKEGKILINKN
                     YVEYKAEKVDKNRPVTYAGFKWNGKAFACSMAGLYACAHYCGVWAIVNPIAGGVCEAV
                     CGTAFAAGCSVG"
BASE COUNT          797 a          309 c          284 g          620 t
ORIGIN      
        1 catcaacatc taaaataaaa aagaataaaa caattaacaa aatactaatt aacaaaccta
       61 aaagtggcaa actttttata tatttttcta aattttcatt cataaaatag actcctttca
      121 aaaatatgtt tataaatata ttaaaataaa tattgatttt ataaagcgct tacttataca
      181 attaaaatat cttattatct tggaggtatc aaatgaaaat tataaaatta ttttcaataa
      241 tttccttgtt cctactggta tttagcgcga atttttctaa tgcttacgca aatgaagaac
      301 aaaaaagttc tttactagaa aatcaaaaag aaaaattgaa aagcaaaaac atcgaagtat
      361 cacaaatgac caagaaagaa ataagttttc tagaacaatt gcaaaataaa aatgaagata
      421 tgcaaaaatt cgaaaagcaa atgaaaaaag aaggatttaa aaaaataaag ctagaagaca
      481 aaaattctaa attaaaatat ctagctacag aagcaaatga taaaaatgca tatggctaca
      541 tagctaataa cgcttataaa aatgaaaaaa caaaagaaat tgtagtttct gaaacaatat
      601 atgataacta caacaaaaaa atcacaaaat tcattgccga aaaaagaagt atgactgaaa
      661 acaaagaggg aaaaatactt attaacaaaa attacgttga atataaggct gaaaaagttg
      721 ataaaaacag accggtaaca tatgcaggat tcaaatggaa cggcaaagca ttcgcttgta
      781 gtatggctgg actttatgca tgtgctcact actgtggagt atgggcaatc gtaaatccaa
      841 tagccggcgg tgtatgtgaa gcagtttgcg gaactgcatt tgcagctgga tgctctgtag
      901 gttgatttta ttccaattgt tttattgacg ttgagcctcg gaacccttaa caatcccaaa
      961 acttgtcgaa tggtcggctt aatagctcac gctatgccga cattcgtctg caagtttagt
     1021 taagggttct tctcaacatc aataaatttt ttcggcataa atgcgtgtct atgcgtagac
     1081 atggagacac ttcagaatta tggagtgatt acatgagttt aactattttt ctttattcag
     1141 ttttaggaag tttattagca aattatatct acgaaaaatc ttcaaaataa ataacttttt
     1201 cggttctact ttgtacactt tatctacctg gtagacctaa ttgttattgc tttacaggtc
     1261 taaaattcgt cgttataatc tagttaaatc ccgtaagggc gcacttatac gtcggataaa
     1321 tcgacatgtg caaaaacgta atcgaccttc gtcgattttt ttatttcaaa attaaagaaa
     1381 cggcttgaga tattccgagc agttataaaa attaatttgc gttgaatatt gctactacat
     1441 tcttgaaaaa gtgatgtaga tttatagccc ataacttttt tgaattacat cgacgatacg
     1501 gcgtcgaaag aaaaataaat agaccgtatg aaagaaaaag ctacctcact ttatctgggg
     1561 tagcttttat tttttatgcc cttattttga attttaagag ggcattttaa agatttaggg
     1621 gtgattcata tagttttata tctaaaactt atatatgctt ttaaaacgca aatatgagcc
     1681 aaataaatat atgctatttt caaaaacaaa aatttgagca aaaacaataa ctatttttag
     1741 acactgccca tttacatgca aattaaaaat tagcataaaa ttctttaaaa cttccccacg
     1801 tttttctaaa ttcctgtagg gcgtttttat caaaaatcaa gacaagaaga aactcgttca
     1861 cactcgtttc aaaaaaacaa gaaatctaat ctatacacct accgttttcc aaccccctca
     1921 aagcatacaa attaaaaaca aaattattca taatcttact ttcttttaac aaaaacataa
     1981 aaaccataaa tagacaaatt aactatcaaa
//
LOCUS       DALAGD010000190         1876 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.198, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000190 DALAGD010000000
VERSION     DALAGD010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.198"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(<1..401)
                     /locus_tag="O2701_002768"
     CDS             complement(<1..401)
                     /locus_tag="O2701_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCZ4294621.1"
                     /translation="MLCETENINKNPKYRIIKYKDEYLMIDLVSTWLALFFPMINWLI
                     PKKYVKISEKDFETLNIVKTAKINSFWPVAGSTVLFGVMLRRYSHLFIVKYEYSIVIL
                     ICCIIILGIFLFFLYLNQKLKLQIYNENKNK"
     gene            complement(525..1154)
                     /locus_tag="O2701_002769"
     CDS             complement(525..1154)
                     /locus_tag="O2701_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HCZ4294622.1"
                     /translation="MLLCDVRVIYKNPKYKVIQHNGEYLLVDLVSTWFVYFFPFINWF
                     IPKKYAIISEEEFENLNVVKPNKNNVFWSVIGSSVLFGVTLRKYIHVFDVQLDKLVVM
                     ILCALALICVIVFYFNLNRKLKLKVFDTNIEKNKRVILIPTFKLGCFLVFGYIFAGSF
                     SIFSLIALMTIEPQNIIIFIYWIMMTMLFFLLNMTSIGNEKVRVIMKNN"
     gene            complement(1607..>1876)
                     /locus_tag="O2701_002770"
     CDS             complement(1607..>1876)
                     /locus_tag="O2701_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCZ4294623.1"
                     /translation="KLTLKIFNTNVVNKNRVVLIPTFKQGLLIVFAYFFLGSASIFTL
                     TILLTTESQNIIIFLTWVIITMFFFLVNMASIGNKNVHVILRINE"
BASE COUNT          683 a          309 c          200 g          684 t
ORIGIN      
        1 cttttatttt tgttttcatt atagatttgt aactttaact tttgatttaa atataaaaaa
       61 aacagaaaaa tacctagtat tatgatgcaa caaattaaaa ttactatact atattcatat
      121 ttaacgataa ataaatggga ataccttctt aacataacac caaataagac cgtacttcct
      181 gccactggcc aaaaagaatt gattttagct gtcttcacaa tgtttaaagt ttcaaaatct
      241 ttttcgctga ttttgacgta cttttttgga atcagccaat taatcattgg gaaaaagagt
      301 gctaaccatg tacttactaa atcaatcatc aaatattcat ctttgtattt gataattcta
      361 tatttgggat tcttattaat attttcagtt tcacaaagca atgtctccac ttcttttcaa
      421 ttggttttat attgatacac tttcatatac attagaagtc atttaaattc tactagcgtc
      481 ttaaaaggat tgtgtcgaca acatttagaa tattttaaat gtaatcaatt atttttcatt
      541 ataacgcgaa ctttttcatt acctatcgaa gtcatattta acaaaaagaa aagcattgtc
      601 atcataatcc aataaataaa tattattata ttttgaggtt cgattgtcat aagggcaatt
      661 aatgaaaata ttgaaaaact tccagcgaaa atatatccga aaactaaaaa acagccaagt
      721 ttaaacgttg gtattaatat aactctctta tttttttcaa tatttgtatc aaacactttt
      781 aacttaagct ttctatttaa gttaaaataa aaaactataa cacaaattaa agcgagagca
      841 cacaatatca ttacaactag cttatctaat tgaacatcaa aaacatgtat gtatttcctt
      901 aaagtaactc caaacaaaac cgaacttcct ataactgacc agaaaacatt atttttattt
      961 ggtttaacaa catttaaatt ttcaaattct tcttcgctaa ttatcgcgta cttttttgga
     1021 atgaaccaat taatgaaagg aaaaaagtac acgaaccaag tgcttactaa atcgactaat
     1081 aagtattcac cgttatgttg aatgactttg tatttcggat ttttataaat gactctgaca
     1141 tcgcaaagca acatttacca ctcccgtttc attagtttta aatgatcccc aatacttttt
     1201 gtaccttcac caactatcgt tccaacacta tctacagctt tttctgtggt atttttaaac
     1261 agcgtctatt taatgtctta tccaattagt tttatctctt aatccaaatt tattagtatg
     1321 aaaagtccaa tttttaagaa ttgtctaaac agattccgca acaaaaccag ctaaaaccgt
     1381 ccatactatt ggattaaaaa cgccaatgtt ataagagcta tcatacaaaa taacacgctc
     1441 tatatttcat ttttaaatta aaacagtttt ttcctttatc aatcacaatg ggcattttca
     1501 cttggtatat tcaaacatta ctatctattt tcctgattta agatatgatt atattttttt
     1561 gaaccaccgt ttaaatatgc gatactacca atttcgatca tttttactac tcgtttattc
     1621 ttaaaataac atgaacattt ttattaccta tcgaagccat attcactaaa aagaaaaaca
     1681 tcgtgataat aacccaagtt aaaaatatta ttatattttg tgactctgtc gtcaaaagaa
     1741 tggttaaagt aaatattgaa gcgcttccta aaaaaaagta cgcaaaaact attaacaatc
     1801 cttgtttgaa agtcggtatt aatacaactc tattcttatt aaccacgttg gtattaaaaa
     1861 tttttaatgt taattt
//
LOCUS       DALAGD010000191         1803 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.199, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000191 DALAGD010000000
VERSION     DALAGD010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.199"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(101..430)
                     /locus_tag="O2701_002771"
     CDS             complement(101..430)
                     /locus_tag="O2701_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294624.1"
                     /translation="MARTYNVIKYVIKKNKDDLKIHGLRLYEFNIKDEKAINISQPIF
                     VNRATLIEKIQNGEAFTHAFRVNHNTYYAGQLISLSLNKNGYINNNFEASRDIIRNIE
                     VSILDYE"
     gene            complement(522..713)
                     /locus_tag="O2701_002772"
     CDS             complement(522..713)
                     /locus_tag="O2701_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294625.1"
                     /translation="MYYLAYGLTTLDNCFKYYQLITQNEHFCYSQQEERESYQQKFQS
                     MINGLKIKIGIQSSNDDAQ"
     gene            complement(810..1775)
                     /locus_tag="O2701_002773"
     CDS             complement(810..1775)
                     /locus_tag="O2701_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCZ4294626.1"
                     /translation="MSISEYKNRYLESLYNTFKTELENERTRYFILDTNYTNSDDTEK
                     EFTWDVKQNNKIREGDLFIFRRPTNLSQIAQQFYFFGAGKIEKIERKNNVATAYISKP
                     LLFVDRVLKDNIENLKWEFKERIKEEWEQFFFKNKISNITKNDFLKLLSLSRNVVEIE
                     SNYINNDISLTFKQMADMATRIKKNIYYVGNHEAFVQAKGSAHFEFSKRIKQNYSYRC
                     AITGIKTKDFLVVTHIIPWHENEFIRLDPSNGICLSLFLAKAFKKGFITFSNSYRVVL
                     SKEAEKDAALYEELKIYENQKIELPDCQKPNLKYLDWHREHIFKN"
BASE COUNT          586 a          296 c          193 g          728 t
ORIGIN      
        1 attaaaattc tattaaaaaa ataagtcact tatatatcaa cttattaagg gagtcctata
       61 ctcccttttt taattgtaat gtattctatc agtattacct ttattcatag tctaaaatac
      121 ttacttcaat gtttctaata atatctcgtg aggcctcaaa gttgttatta atataaccat
      181 ttttattcaa tgataaagaa attaattggc ctgcataata agtattgtga tttactctaa
      241 aggcgtgcgt aaatgcttca ccattttgta ttttctcaat caatgttgct ctgttcacaa
      301 aaatgggttg gctaatattt atagcttttt catcttttat attaaattca tataatctta
      361 atccatgtat tttaagatca tccttatttt tcttaatgac atatttaatc acattatacg
      421 ttctagccat ttactaatct tcctccatta agatattaat tcgaagctct ttcttctttt
      481 aaaaacatat aacgttcaat taaaaaatca aactttgtag cttattgcgc atcatcattt
      541 gaactttgta tgccaatttt aatttttaaa ccattaatca tactttgaaa tttttgctga
      601 taactttctc tttcttcttg ttgagaatag caaaaatgtt cattttgtgt aattaattgg
      661 taatatttaa aacaattatc taaagttgtt aatccatagg ctaaataata caaattatct
      721 gtttcaagtg ctctttttca atctactaat gaaagatgta tggcacctct actaattgac
      781 taatatctgt catacaacct ctcccaaaat taatttttaa aaatatgttc tctatgccaa
      841 tctaaatatt tcaaattagg tttctgacaa tctggtagtt cgattttttg attttcataa
      901 atctttaatt cttcatataa agcagcatct ttctctgctt ctttagataa aacaactcta
      961 taactgttag aaaaagtgat aaagcctttc ttaaatgctt ttgctaaaaa taaagacaaa
     1021 caaataccat ttgaaggatc tagtctaata aactcatttt catgccaagg aataatatgt
     1081 gtaacgacta aaaagtcttt agttttaatc ccggtaattg cacatctata actataattt
     1141 tgtttaatcc tttttgaaaa ctcaaaatga gcacttccct tagcttgaac aaatgcttca
     1201 tgattcccta cgtagtaaat atttttcttt attcgagttg ccatatcagc catttgttta
     1261 aaagttagcg aaatatcatt attaatataa ttactttcta tttccactac atttctactc
     1321 aaacttagta gttttaaaaa atcatttttt gtaatattac tgattttatt tttaaagaaa
     1381 aactgctccc actcttcttt aattctttct ttgaactccc attttaaatt ttcaatatta
     1441 tcttttaaaa cacgatccac aaaaagtaag ggttttgaaa tgtaagcggt tgctacatta
     1501 ttttttcgct caattttttc tattttccca gctccaaaga aataaaattg ttgagcaatt
     1561 tgagataaat tagttggccg tctaaaaatg aacaaatcgc cttctctgat cttattattc
     1621 tgttttacat cccaagtaaa ctccttttca gtatcatcac tatttgtata gttggtatct
     1681 aatataaaat agcgagtgcg ttcgttttct aactcagttt taaaggtgtt atatagtgac
     1741 tctaagtatc tatttttata ttcgcttata ctcatctttc tatcagctca cttgtaaatt
     1801 ata
//
LOCUS       DALAGD010000192         1800 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.200, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000192 DALAGD010000000
VERSION     DALAGD010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.200"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            81..215
                     /locus_tag="O2701_002774"
     CDS             81..215
                     /locus_tag="O2701_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lysin family phenol-soluble modulin"
                     /protein_id="HCZ4294627.1"
                     /translation="MSCLILRIFILIKEGVISMAQDIISTIGDLVKWIIDTVNKFTKK
                     "
     gene            complement(812..1597)
                     /locus_tag="O2701_002775"
     CDS             complement(812..1597)
                     /locus_tag="O2701_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HCZ4294628.1"
                     /translation="MKVQIYQLPIVFGDSSKNETQITQWFEKNMNAEVDVVVLPEMWN
                     NGYDLEHLNEKADNNLGQSFSFIKHLAEKYKVDIVAGSVSNIRNNQIFNTAFSVNKSG
                     QLINEYDKVHLVPMLREHEFLTAGEYVAEPFQLSDGTYVTQLICYDLRFPELLRYPAR
                     SGAKIAFYVAQWPMSRLQHWHSLLKARAIENNMFVIGTNSTGFDGNTEYAGHSIVINP
                     NGDLVGELNESADILTVDLNLNEVEQQRENIPVFKSIKLDLYK"
BASE COUNT          642 a          285 c          268 g          605 t
ORIGIN      
        1 agttaagaaa aattggaaaa taaatgcttt tagcatgttt taatataact agatcacaga
       61 gatgtgatgg aaaatagttg atgagttgtt taattttaag aatttttatc ttaattaagg
      121 aaggagtgat ttcaatggca caagatatca tttcaacaat cggtgactta gtaaaatgga
      181 ttatcgacac agtgaacaaa ttcactaaaa aataagatga ataattaatt actttcattg
      241 taaatttgtt atcttcgtat agtactaaaa gtatgagtta ttaagccatc ccaacttaat
      301 aaccatgtaa aattagcaag tgagtaacat ttgctagtag agttagtttc cttggactca
      361 gtgctatgta tttttcttaa ttatcattac agataattat ttctagcatg taagctatcg
      421 taaacaacat cgatttatca ttatttgata aataaaattt ttttcataat taataacatc
      481 cccaaaaata gattgaaaaa ataactgtaa aacattccct taataataag tatggtcgtg
      541 agcccctccc aagctcgcgg ccttttttgt aatgaagaag ggatgagtta atcatcatta
      601 tgagacccgc cgttaaaata tatgaataag tctaatgttg gaaaaggtca aaaaattaat
      661 caatttaatt aagaaaatca ttcatttgca aagggcgaaa tgggttctta ctgagttatc
      721 tattataaaa aaataaacat agacttatga aaaatctctc ataaatctat gtttagtcat
      781 gacatgtgtt aaatattatt tcgggcgctt cttatttata caaatctaat ttaatacttt
      841 taaatacagg tatattttcg cgttgctgtt ctacttcatt taagtttaaa tctacagtca
      901 aaatatctgc ggattcattt aattctccaa ctaaatctcc atttgggttt ataactatcg
      961 aatgaccagc atattctgtg ttaccatcga atccagtgct attagttcca atgacaaaca
     1021 tattattttc aattgcacgt gcctttagta atgaatgcca atgttgaaga cgtgacatag
     1081 gccattgcgc cacataaaat gcaattttag caccactacg agcaggatat cttaataatt
     1141 ctggaaaacg taaatcataa cagataagtt gggtcacata agtaccgtca gacaattgaa
     1201 agggttcagc tacgtattcg ccagcggtta aaaattcatg ctctcttaac ataggaacta
     1261 aatgaacttt gtcgtattca ttaatcagct ggccactttt attcacacta aaagctgtat
     1321 taaatatttg attgtttcta atgttagaaa ctgacccagc tacgatatcg actttatatt
     1381 tttcagctaa atgtttaata aatgaaaaac tttgtcctag attattatct gctttttcat
     1441 ttaaatgctc taaatcatag ccattattcc acatttcagg taaaacgact acatctactt
     1501 cagcattcat atttttttcg aaccattgcg ttatttgagt ttcattttta gaactatctc
     1561 caaaaacaat cggtaattga taaatttgga ctttcataac atcacatcct tgatagatct
     1621 tatatataac ttactaaaag ttatgttgaa acgcaaaaaa cgagcacaag acataaaatc
     1681 aaagtcctag gctctacaaa gttatattga cagtagttga tggggcccca acatagagaa
     1741 attggaacac caatttctac agacaatgca agttggggtg ggctctaaca taaagaaata
//
LOCUS       DALAGD010000193         1726 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.201, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000193 DALAGD010000000
VERSION     DALAGD010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.201"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            221..331
                     /locus_tag="O2701_002776"
     CDS             221..331
                     /locus_tag="O2701_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294629.1"
                     /translation="MKMTIKVRKTNKKEKIEFLIGTFIIILVILGFKIMK"
     gene            759..944
                     /locus_tag="O2701_002777"
     CDS             759..944
                     /locus_tag="O2701_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294630.1"
                     /translation="MSEYKKKIIELIESNLTGYEISKKTGVSQYVLSQLRQGKREVDN
                     LTLNTTEKLYEYANKVL"
     gene            1456..1560
                     /locus_tag="O2701_002778"
     CDS             1456..1560
                     /locus_tag="O2701_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294631.1"
                     /translation="MFKVNYSILSYYPEYNIAVSWQRLREGKTIKNKI"
BASE COUNT          722 a          170 c          280 g          554 t
ORIGIN      
        1 attaattttt cgagtgtaaa gaatcgtgtg gtaaacataa tttatataga ttataaggag
       61 gtgaaaatgg tcgaggaagt taaaacgata aaatagtttg aatgttttga tttcaattct
      121 ggtaggacta ttgaatttgc aatctaataa gaatgtgagg tttgatttaa tatgaagtca
      181 tattctttga ataaaatatt actttctcac cattagtaaa atgaagatga ctattaaagt
      241 gcgaaaaaca aataaaaaag agaaaataga atttttaata gggacgttta taattatttt
      301 agtaatatta gggttcaaaa ttatgaaata aatgtatagt atttgagaag tctatatgac
      361 tccggaaaaa ttaaattcta aagatatctc aatatctcaa atttaataga ctactggagt
      421 tagtagtttg aatattgaaa gacactaatc taataaaaat acataaaagg ttaaacttta
      481 gaagtggctg taaacttcaa tgaatactaa acattgaagt tttgtaaaag gcgtaaggta
      541 tacaagacaa atcgtttatc tttgacaaag taattgatga tgaaaagtcg ttaatatgta
      601 gaaacaaaaa ttgaaaatat aaaatttaac tattatgaag tgatgatagc cattaatgaa
      661 tagaaaaata ataaaatcat ttatagataa aaaatacgtt aagttcacag aatttcgaaa
      721 aaaagcagaa aacattgtac agatatggag atgaaaaaat gtcagaatac aagaaaaaaa
      781 taattgaatt aattgaaagt aatttaacag gatatgaaat ttctaaaaaa actggagttt
      841 ctcaatacgt actttcacaa ttaagacagg gcaaacgcga agtagataat ctaaccctga
      901 atacaacaga aaaattatat gaatatgcca ataaagtttt gtaatttaac taatgtataa
      961 attaatcaag ctatgtttat ttgatttaac tattaataaa aatcatatgg tgaatggata
     1021 ttataataat taaaatacaa aaatagtaga ttccaatttg tgaaaaagga gtatgtcgtt
     1081 tcatttagaa tttattgcac gaaattagca tcgaataagg gaaaataagt gttaagtttt
     1141 aaatgataaa aaagattgga atggatcgtc ttgaaatgct cccttcaaag ttttcatttt
     1201 ttcaatgtcg acttcgaagg gggcattttc attaaattgt tatagctttt tatatttgta
     1261 taatgaacat ataagtttaa gaaggtgcga gtgaaggaaa taaaaaagct caaatgtacc
     1321 aaattgttaa tcttaataaa tctctacttt ataaagattg aatggacatt cgagcgttaa
     1381 tcagtcagga gggactttcc ctcctacaat ttaataataa tacttgcttc accactatac
     1441 aaggagtgag ttgttatgtt caaagtgaat tattcgattt taagttatta tccagaatat
     1501 aatatcgcag taagttggca acgtttaaga gaaggaaaaa caataaaaaa caagatttaa
     1561 tactgctgcg tcatgaggcg cttgaacatt atttgatgaa taagtataat ttcaactatg
     1621 attatgcaca taaaattgta tcaaaaaaat acgattattc aatttttata aaaaagaagg
     1681 tggattaaat gcttacatta ataaaattga aagaagatga acaggt
//
LOCUS       DALAGD010000194         1719 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.202, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000194 DALAGD010000000
VERSION     DALAGD010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.202"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            364..765
                     /locus_tag="O2701_002779"
     CDS             364..765
                     /locus_tag="O2701_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl peptide receptor-like 1 inhibitory
                     protein"
                     /protein_id="HCZ4294632.1"
                     /translation="MKKNITKTIIASTVIAAGLLTQTNDAKAFFSYEWKGLEIAKNLA
                     DQAKKDDERIDKLMKESDKNLTPYKAETVNDLYLIVKKLSQGDVKKAVVRIKDGGPRD
                     YYTFDLTRPLEENRKNIKVVKNGEIDSITWY"
     gene            725..817
                     /locus_tag="O2701_002780"
     CDS             725..817
                     /locus_tag="O2701_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294633.1"
                     /translation="MLKTVKSTRLLGIKKHTELNSCTPNVRKQC"
     gene            complement(1134..1463)
                     /gene="ecb"
                     /locus_tag="O2701_002781"
     CDS             complement(1134..1463)
                     /gene="ecb"
                     /locus_tag="O2701_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement convertase inhibitor Ecb"
                     /protein_id="HCZ4294634.1"
                     /translation="MKKNFIGKSILSIAAISLTVSTFAGESHAQTKNVEAAKKYDQYQ
                     TNFKKQVNKKVVDAQKAVNFFKRTRTVATHRKAQRAVNLIHFQHSYEKKKLQRQIDLV
                     LKYNTLK"
BASE COUNT          619 a          252 c          217 g          631 t
ORIGIN      
        1 ctttgtttta taaaaaatat atatcaaaac agctatatgt ataacaattt ggagtgtttg
       61 tctatccaac ttagcaaaca aaatgtagag aaatgtaaat atcgttttag caataaaaat
      121 tattaaatca tagttgagtg acgtttacta ctattattat actatcaaca aattcaataa
      181 tacctcaaat aaaaataata ccatcaaatt catcattatt ctttttcgat tgcattaaac
      241 cttattaatt tgcacttaat tttaattaat gaagtgttaa ctggtaaatg tatgaaaccg
      301 tttgtaaaat agcagtgtac tcgcattagt aagtacaaat ttattttaca gggagaactt
      361 attatgaaaa aaaatatcac aaaaactatt atcgcatcta cagtaatcgc tgcaggtctt
      421 ttaactcaaa ctaatgatgc taaagctttc tttagttatg aatggaaagg tttagaaatc
      481 gcaaaaaatt tagcagatca agctaaaaag gacgatgaac gtattgataa gttaatgaaa
      541 gagtctgata aaaatctaac tccttacaaa gctgaaactg ttaatgatct gtaccttatt
      601 gttaaaaaat taagccaagg tgatgtgaag aaagcagttg tcagaattaa agatggtggt
      661 cctagagatt actatacttt tgacttaact cgtcctttag aagagaacag aaaaaatatt
      721 aaagttgtta aaaacggtga aatcgactcg attacttggt attaaaaaac atactgaatt
      781 aaatagttgt acgccaaacg ttagaaaaca atgctaacgt attggcgtgc ttttttattt
      841 taagtaactt ccaatttatt tagcatcttt acgactgttt aataaagcac gtatgattaa
      901 cacggttgcc actatatccg ttacaatttt tatgattgtt aatacattcg atttcctttt
      961 cacaatagcc acctcgcttg ttcaaaacat aaacaactat tgcattcact tttaagtaat
     1021 tatttatatt atttatccca ataagctcac cattcaaata aacacaatat tataaaataa
     1081 ttataccttt ggatatagca aaaagccaca ctctatagca tggcttctat caattatttt
     1141 aaagtattat attttaaaac tagatcgatt tgtctttgta attttttctt ttcataactg
     1201 tgttggaaat gaattaaatt aacagctctt tgtgctttac ggtgtgttgc aacagttctt
     1261 gtacgtttga aaaagtttac agctttttgt gcatccacaa cttttttatt tacttgtttt
     1321 ttaaagtttg tttgatactg atcatatttt ttagcagctt caacgttttt agtttgtgca
     1381 tgagattcac cggcaaacgt tgataccgtt aaactaatag cagctatgct taaaattgat
     1441 ttcccaataa aattcttttt cataattaat aacacctatc ctcaaatatt tattttgtat
     1501 atatttataa taatagacag atacaaaaaa ctacattaac tatttattaa ctaaacatta
     1561 atatttgtgt cactacattt ctgaatttca agaattttgc tactccatat tttaaccatc
     1621 aaattattga tataatttat gactttagaa atccttttaa atatgtatac gattcatgat
     1681 tttaactaaa atttcaaatt tcctgacttc ttggaatct
//
LOCUS       DALAGD010000195         1675 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.203, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000195 DALAGD010000000
VERSION     DALAGD010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.203"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(376..576)
                     /locus_tag="O2701_002782"
     CDS             complement(376..576)
                     /locus_tag="O2701_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCZ4294635.1"
                     /translation="MNNGTVKWFNAEKGFGFIEREDGSDVFVHFSAIAEDGYKSLEEG
                     QKVEFDIVEGDRGEQAANVVKM"
     gene            complement(933..1619)
                     /locus_tag="O2701_002783"
     CDS             complement(933..1619)
                     /locus_tag="O2701_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCZ4294636.1"
                     /translation="MTEYLLSAGICMAIVSILLIGMAISNVSKGQYAKRFFYFATSCL
                     VLTLVVVSSLSSSANASQTDNGVNRSGSEDPTVYSATSTKKLHKEPATLIKAIDGDTV
                     KLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQR
                     TDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNTHEQLLRKSEAQAKKEKLNI
                     WSEDNADSGQ"
BASE COUNT          527 a          299 c          208 g          641 t
ORIGIN      
        1 aaattaacaa aaagttgatt agttaaatta ttaattaagt agtaagatat tatgcgatgt
       61 ggtatcctat tattgttaaa cattttgagt gtttaacatt acttcttgtt atcgccatat
      121 acagacttta attctcgtca ttattaaagt tctgtattgg cgctttttta tattcaaaat
      181 caaaagtcgg acagatgaaa agtaaaaaac ttttccatca gttcgattta ttttagaacc
      241 attatatatt gttaaatctc caactaaatt tattggacaa aaaaataacg gctagtgctt
      301 ttaccacacc aactgttatc gatttaaagc ataaaaaaac agccctatag taaggactgt
      361 ttgaataagt taaaattaca ttttaactac gtttgcagct tgctcgccac ggtcgccttc
      421 aacgatgtcg aattcaactt tttggccttc ttctaatgat ttgtatccat cttcagcgat
      481 tgctgagaag tgtacgaata cgtcgctacc atcttctctt tcgatgaaac caaaaccttt
      541 ttctgcatta aaccatttaa ctgtaccgtt attcatattg aatacctccg tcgtgctttt
      601 gcactaaata ttttgtaaca aattcataaa tgaaaaggag aatattctat ataaataact
      661 cacatttttc ttcacgctct ttattactta actttcatta tacactttta aatatagaag
      721 taaagcatta ttttattttc tgtgataata cacattgaac tatatagtaa cattccattt
      781 taatactaaa tttaaaattt ttattttcta atattaaata cacttacttt tgatactatt
      841 tactttttta attctgaatt gataataaat catatcgtga tctaaaaatt ataaaagtgc
      901 cactagcagc agtgacactt ttacaatgag cattattgac ctgaatcagc gttgtcttcg
      961 ctccaaatat ttaatttctc tttttttgct tgtgcttcac tttttcttaa aagttgttca
     1021 tgtgtattgt taggtttata aacataagca actttagcca agccttgacg aactaaagct
     1081 tcgtttacca tttttccatc agcataaata tacgctaagc cacgtccata tttatcagtt
     1141 ctttgacctt tgtcaaactc gacttcaatt ttctttgcat tttctaccat ttttttcgta
     1201 aatgcacttg cttcaggacc atatttctct acaccttttt taggatgctt tgtttcaggt
     1261 gtatcaacca ataatagtct gaatgtcatt ggttgacctt tgtacattaa tttaaccgta
     1321 tcaccatcaa tcgctttaat taatgtcgca ggttctttat gtaatttttt agttgaagtt
     1381 gcactatata ctgttggatc ttcagaacca cttctattta cgccattatc tgtttgtgat
     1441 gcatttgctg agctacttag acttgaaact acaactaaag ttaacactaa acaactagta
     1501 gcgaaataga aaaacctctt tgcgtattgc cctttcgaaa cattactgat agccatccct
     1561 ataagtaata ttgaaacgat tgccatacat atgccagcac ttaataagta ttctgtcata
     1621 actaacacct ctttcttttt agttaatttt aatattaaac gttaattaat tataa
//
LOCUS       DALAGD010000196         1665 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.204, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000196 DALAGD010000000
VERSION     DALAGD010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.204"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            74..565
                     /locus_tag="O2701_002784"
     CDS             74..565
                     /locus_tag="O2701_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCZ4294637.1"
                     /translation="MTFEEKLSEMYSEIANKIISMIPVEWEKVYAMAYIDEECGEVFY
                     NYTEPSSDELFYYTSVIKKYNLLKSSFMDSVYELHDQFEELREVFIEEGLEPWTSCEF
                     DFTKEDKLKVSFDYIDWINTEFDQLGRENYYMYKKFGVLPETEYEMEEVKEIEQYIKE
                     QEE"
     gene            775..1458
                     /locus_tag="O2701_002785"
     CDS             775..1458
                     /locus_tag="O2701_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HCZ4294638.1"
                     /translation="MTTKENIDILRKPGAQALSLASLFMILFSCLTFFFGLDYERFPN
                     YLKITTIIELIIIIISLLQWIRFIDFEKESAQKYKKIYARFLVIINVLTTITAVFATC
                     NLYYFVAVQNHYDLFNYWLMGTISIIISYLLLVIGGMFTLLKLPKVTKRWGGKTKTHF
                     GLLLTALSAFIYIERIIEYILVPNVVESKFVIMVSIIIIACTQFVAFQFIMQYSRFYI
                     FELNTEDDD"
     gene            1580..>1665
                     /locus_tag="O2701_002786"
     CDS             1580..>1665
                     /locus_tag="O2701_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 domain-containing protein"
                     /protein_id="HCZ4294639.1"
                     /translation="MIIRIRKGTFAFVVINLLFFNCWLFILN"
BASE COUNT          633 a          173 c          283 g          576 t
ORIGIN      
        1 gaaattaata aagttaaaga aatcgagcaa tatattaaag agcaagatga agctgaacta
       61 taggggcgat aatatgactt tcgaagaaaa attaagtgaa atgtatagcg agattgcgaa
      121 taagattatt agcatgatac cggtagagtg ggaaaaagta tatgcaatgg catatatcga
      181 tgaagaatgt ggagaagtgt tttacaatta tactgaacca agcagtgatg aattatttta
      241 ctatacaagt gtgataaaga agtataattt attgaaatca agctttatgg attcagtata
      301 tgagttgcat gatcaatttg aggaactaag agaagtattt atagaagaag gtcttgaacc
      361 atggacatca tgcgaatttg attttacaaa agaagataaa ttaaaagttt catttgatta
      421 tattgattgg ataaatacag agtttgatca attaggccgt gaaaattatt atatgtacaa
      481 aaaatttggt gttttaccag aaacggaata tgaaatggag gaggttaaag aaatcgagca
      541 atatattaaa gagcaagaag aatagacaat cttaatgtaa gattaaacaa taaagctttg
      601 tttagtcttt ttagcgttta agtaaaaaac aatagagacc ataaagttgt catcaaataa
      661 taatataaag ataattttag gcttttatac ttttaatcga aaagaatcta agtacttata
      721 actttaaata cttaatatgc tattacaaaa tcaatgtata ggagataggt aaatatgact
      781 actaaggaaa atatagacat tcttcgaaag ccaggtgcac aagccttaag tttagcatca
      841 ttatttatga tacttttttc atgtctaact ttcttttttg gtttagatta tgaaaggttt
      901 ccaaattatt taaagataac gacaattata gaattaataa ttatcataat tagtttactt
      961 caatggatta gatttataga tttcgaaaag gaaagcgcac agaaatataa gaaaatatat
     1021 gcccgatttt tagttattat aaatgtgcta actactatca ccgcagtatt tgcaacatgt
     1081 aacctttatt attttgttgc tgtacaaaat cattatgacc tattcaatta ttggttgatg
     1141 ggtacgattt caatcataat tagttatttg ttattagtaa ttggcggaat gttcacgtta
     1201 ttaaaattac ctaaagtaac aaaacgttgg ggtggtaaaa ctaaaacaca tttcggttta
     1261 ttattaaccg cgttgagcgc atttatatat attgaaagaa ttatcgaata tatattggtg
     1321 cccaatgtcg tagaatccaa gtttgtcata atggtaagta tcattattat tgcttgtaca
     1381 caatttgtgg catttcaatt tattatgcaa tacagtagat tctatatttt tgaattaaac
     1441 actgaagatg atgactaaat aattttataa aatactgttg gagtatactt aaataagaaa
     1501 atcaacttca acgtatatca ccatattatt ttatactcaa tgatgtgaaa gaatttgagt
     1561 ggattgatat taatgggatt tgattattag aattagaaaa ggaacgtttg cttttgttgt
     1621 aataaatcta ttatttttca attgttggtt atttattctg aatat
//
LOCUS       DALAGD010000197         1647 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.205, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000197 DALAGD010000000
VERSION     DALAGD010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.205"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..1254
                     /gene="priA"
                     /locus_tag="O2701_002787"
     CDS             <1..1254
                     /gene="priA"
                     /locus_tag="O2701_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCZ4294640.1"
                     /translation="IIDEEHESTYKQEDYPRYHAREIAQWRSEYHHCPVILGSATPCL
                     ESYARAEKGVYHLLSLPNRVNQQALPEIDIVDMREELSEGNRSMFSKDLREAIQLRLD
                     RQEQVVLFLNRRGYASFMLCRDCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQETPPN
                     QCPNCESEHIRQVGTGTQKVEELLQQEFEDARIIRMDVDTTSKKGAHEKLLTEFEKGN
                     GDILLGTQMIAKGLDYPNITLVGVLNADTMLNLPDFRASERTYQLLTQVAGRAGRHEK
                     AGQVIIQTYNPDHYSILDVQKNDYLTFYRQEMEYRKLGKYPPYYYLINFTISHKEMKK
                     VMVASQHVHKILLQHLTEKALVLGPSPAALARINNEFRFQILVKYKSEPGLLQAIQFL
                     DDYYHEKFIKEKLALKIDIDPQMMM"
BASE COUNT          597 a          235 c          294 g          521 t
ORIGIN      
        1 atcattgatg aagaacatga atctacatat aaacaagaag attatccgag atatcacgct
       61 agagaaattg cccaatggcg aagtgaatat catcactgtc cagtcatttt aggaagtgca
      121 acaccatgtc ttgaaagtta tgcacgagct gaaaaaggcg tttatcattt gctatcatta
      181 ccaaacagag tgaaccaaca agctttacct gaaattgata tagtagacat gcgtgaagaa
      241 ttgagtgaag gtaatcggtc aatgttttca aaagatttac gtgaagccat acaattaaga
      301 ttagatcgac aggaacaagt tgttttattt ttaaatcgac gtggttatgc atcgtttatg
      361 ttatgtcggg attgtggata tgtaccgcaa tgtccaaact gtgatatttc attaacgtat
      421 cataaaacga cagacttatt aaaatgtcac tattgtggtt accaagagac gccaccgaat
      481 caatgtccaa attgtgagag tgaacacatt cgacaagtag gtactggtac tcagaaagtt
      541 gaagaactat tgcaacaaga atttgaagat gcgcgcataa ttaggatgga tgtagataca
      601 acctcaaaga aaggtgcaca tgaaaagtta ttgactgaat tcgaaaaagg taacggtgac
      661 attttactag gtactcagat gattgcgaaa ggattagatt atccaaatat tactttagtt
      721 ggtgtgctga atgcagatac aatgttaaat ttacctgatt ttcgggcgag cgaacgtact
      781 tatcaactat taacgcaagt ggctggtaga gctggtcgtc atgaaaaggc aggtcaagtc
      841 atcattcaaa cgtataatcc agatcattat tcaatattgg atgttcaaaa aaatgattat
      901 ttaacatttt atcgtcagga aatggaatat cgtaaattag gaaagtatcc accgtattat
      961 tatttgatta atttcacaat ctcacataaa gaaatgaaga aggttatggt agcatcgcag
     1021 catgttcata aaattttatt acagcattta acagaaaaag cgcttgtact aggtccatct
     1081 ccggcagcac ttgcgagaat caacaatgaa tttagattcc aaattttagt gaaatataaa
     1141 agtgaacctg gattattaca agccattcag tttttagatg actattacca tgaaaaattt
     1201 ataaaagaaa aattagcatt gaagattgat attgatccac agatgatgat gtaacattac
     1261 taattattag aaacaagttc agtatgtacg agtatttgaa ccagtgtttg aatatttact
     1321 ttaagtacag aaaaagggca gaatatacaa ctgttaacta tttaaattag cagtttatat
     1381 tctgtctttt tatatggctt tataacttac gtgattttgg tttgataagg aatttattag
     1441 tattttcatt tacgaattct gattttaatg caagcgccat acttactgat gaataaaagt
     1501 actaatgatt aaacaaacta tttttaaatg gcaaattgaa atagtgcgaa ttttaaactt
     1561 ataaattaat ggctataaac tattttatat ctatctatgt tttaactact taaattatta
     1621 gacaaaacaa ttttcttaaa ttatcaa
//
LOCUS       DALAGD010000198         1625 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.206, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000198 DALAGD010000000
VERSION     DALAGD010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.206"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            114..416
                     /locus_tag="O2701_002788"
     CDS             114..416
                     /locus_tag="O2701_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294641.1"
                     /translation="MSRSKKYFYLSSLMIILSFFFNTNNVFLSGLFNSFIKLILFCSV
                     INSIVLILSIIFADRSIKSLKPDADWIRIASKSLPWIILIVILVHIFSIVRTFGFI"
     gene            672..1037
                     /gene="crcB"
                     /locus_tag="O2701_002789"
     CDS             672..1037
                     /gene="crcB"
                     /locus_tag="O2701_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCZ4294642.1"
                     /translation="MQYVYIFIGGALGALLRYLISFLNTDGGFPIGTLIANLTGAFVM
                     GLLTALTIAFFSNHPTLKKAITTGFLGALTTFSTFQLELIHMFDHQQFITLLLYAVTS
                     YVFGILLCYVGIKLGGDLS"
     gene            1034..1387
                     /locus_tag="O2701_002790"
     CDS             1034..1387
                     /locus_tag="O2701_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HCZ4294643.1"
                     /translation="MISIILVMIGGGFGAIARSAITDYFNHKFTSKLPIATLIVNLVG
                     SFLIGLTIGLSISISWFPAFFVTGFLGGLTTFSTLAKELTLMMTPKFNINLFLNYSLL
                     QFIIGFIACYIGYHI"
BASE COUNT          521 a          249 c          230 g          625 t
ORIGIN      
        1 tatatttttt aaaaatgact ttagcaaaca ttgtgtctac aagttaataa aactattgtt
       61 ttatgattgt ttatgatacg atatgtaaat gataatcatc caggaggtct agcatgtctc
      121 gttcaaaaaa atacttttac ttatctagct taatgattat tttaagcttt ttctttaata
      181 caaataacgt tttcctaagt ggacttttta attcttttat taaattaata cttttctgca
      241 gtgttattaa ctcaattgta ctaattttgt ctataatttt tgcagatcgt tcaattaaat
      301 cactaaagcc tgatgcagat tggattagaa ttgcgagtaa aagtttgcct tggattattc
      361 taattgttat tttagtacat atcttttcaa ttgttcgtac attcggtttt atttaaaaaa
      421 gttaatatgt cattgtagcc ttaatacaaa acaatacaat gtatcatgct ataatgagta
      481 aaacaatttg ataacgttgt tgcgtataaa aaatattaga ttttcgaaat cataactatg
      541 catctaatcg ctatagttat acaacaagat ataacatata atgaggtttg ataatgcatc
      601 gacaattttt gtcgtcgcgt tgccaaaacc tcttttttaa attcaaacta cttcttttcg
      661 aggtgaacca aatgcaatat gtatatattt ttatcggtgg tgctttaggc gctttattac
      721 gttacctcat ttcttttctg aatactgacg gaggttttcc aatcggaaca ctgatagcca
      781 atttgactgg tgcctttgta atgggattgc taacagcctt aacaattgca tttttttcaa
      841 accatccgac cctaaaaaaa gctattacga ctggttttct tggtgcttta acgacttttt
      901 caacatttca attagaatta atacatatgt ttgatcatca acaatttata actttactac
      961 tatatgctgt aacaagttat gtctttggta ttttgttatg ttacgtcggt ataaaactag
     1021 gtggtgattt atcatgatat caatcatttt agtcatgatt ggcggcggtt ttggcgcaat
     1081 tgctagaagt gccattactg attattttaa tcataaattt acttcaaagt tacctatcgc
     1141 aacattgata gtaaatctag ttggtagttt tttaattgga ttaactatag gcttatcaat
     1201 ttcaatctca tggttccctg cgttctttgt taccggtttt ttaggtggct taacaacttt
     1261 ctcaacgtta gccaaagaac ttacactaat gatgacgcca aaatttaata ttaacctttt
     1321 tctcaattat tcacttttac aattcatcat tggatttata gcttgttata ttggctatca
     1381 tatttaaaaa taaaatgctt cattcagcaa ataggtaaat tacgacacct tcctgaacga
     1441 agcatttttt aattttcatg caaattttta agcaccatat aatgcctacc aaatttcaat
     1501 aatctttgtt gccgtttaaa taatgtgaat gtcaataaat tctccaaact agtcgaaaat
     1561 aaagggagtg ggacataaat ccctaaaaaa aagcagtaag ataattttca attagaaaat
     1621 atctt
//
LOCUS       DALAGD010000199         1571 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.207, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000199 DALAGD010000000
VERSION     DALAGD010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.207"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..>1571
                     /locus_tag="O2701_002791"
     CDS             <1..>1571
                     /locus_tag="O2701_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCZ4294644.1"
                     /translation="EEEFEEKKKYLSEIYELIDEINIDPYIFLTEHNECNIYKISENE
                     KLELQTYMSKIEKVLLKYQTYLKETCKECLFYPYLLVQGEAGIGKSHLLAHLSKKLRD
                     ENHIIYLFLGQFFTKNEDPWHQILNDLEVTNSVDNFLRSISNKAKETKKRAFIIIDAL
                     NEGEGKRLWGNYFQSFINHIKKYSNIALIFSIRTPFEDVILPKNAIQDNNIVVFQHEG
                     FSKEENYNPIVSFCDFYGLELPKLPILNPEFNNPLFLKLMCEYCVNKFKEFDQTISVA
                     ELFTNVLKTVNINLSKEDKFDFDKNINVVQKVIKGLVELMNDSEFNQLNYEESYTVVN
                     NIAKEYVQKSNRFLEALIDENILIKNTGYKGEMIIYFSYERMGDYFLSEYLLEKYRNV
                     DKRDLVTKLQSDEKVTRYFQKEDDLSYNRGLINELFIKLANEFNIELFEVFPQFKNNY
                     NMIYSFINSLVWRKDGSISKHTKCYISDNVIPYDAFRNNFLDVLLIKMPQKNHPLNIW
                     ALHKLLKQCNLGKRD"
BASE COUNT          643 a          157 c          236 g          535 t
ORIGIN      
        1 gaagaagagt ttgaagaaaa gaaaaaatac ttaagtgaaa tttatgaatt gatagacgaa
       61 attaatattg acccttatat ttttctgaca gaacataatg aatgtaatat atacaaaata
      121 agtgagaatg aaaaattaga acttcaaact tatatgagta aaatcgaaaa agtattatta
      181 aaatatcaaa cgtatttaaa agaaacatgt aaagaatgct tgttttatcc ttatttgcta
      241 gttcaaggtg aagctggcat aggaaaatca caccttttag cgcatctatc taaaaaactt
      301 agagatgaaa atcatattat ttatctgttt ttgggacaat tttttactaa aaatgaagat
      361 ccatggcatc aaatacttaa tgatttagaa gttacaaatt ctgttgataa ttttttaagg
      421 tcaataagta ataaggccaa agaaacaaaa aaaagagctt ttattattat tgatgcgctt
      481 aatgaaggtg aaggtaaaag gttatgggga aattattttc aaagctttat caaccatata
      541 aagaagtatt ctaatatagc tttaatattt tctattagaa ccccttttga agatgttata
      601 ttacccaaaa acgcaataca agataacaat attgtagtat ttcagcatga aggttttagt
      661 aaggaagaaa actataatcc aattgtatct ttttgtgatt tttatggatt agagctacct
      721 aagttaccta tattaaatcc agaattcaac aatccattat ttttaaaact aatgtgtgag
      781 tactgtgtaa ataaatttaa ggaatttgac caaactataa gtgttgcaga gctatttaca
      841 aatgttctta agacagtaaa tattaattta tctaaggaag ataaatttga ttttgataaa
      901 aatattaatg ttgtacaaaa agtaataaaa ggattagttg aattaatgaa cgactcagaa
      961 tttaatcaac taaattatga ggaatcctat actgttgtta ataatatagc taaagagtat
     1021 gttcaaaagt caaatagatt tttagaagcg ctaatagatg aaaatatatt aattaaaaat
     1081 actggctata aaggtgaaat gattatttat ttttcgtatg agcgtatggg agattacttt
     1141 ttatcagaat accttttaga gaaatataga aatgttgata aaagagattt agtaactaaa
     1201 ttacagtcag atgaaaaagt gacaaggtat tttcaaaagg aagacgattt atcttataat
     1261 agaggtctta ttaatgaact ttttattaaa ttagcaaatg aatttaatat agagctattt
     1321 gaagtatttc cacagtttaa aaacaattat aacatgatat attcttttat aaatagttta
     1381 gtatggagaa aagatggtag cataagtaaa catactaaat gctatatatc cgataatgtt
     1441 attccttatg atgcatttag aaataatttt ttagatgtct tattaataaa aatgcctcaa
     1501 aaaaaccatc cgttaaatat ttgggcttta cataaattat tgaaacaatg caatttagga
     1561 aaaagagatt t
//
LOCUS       DALAGD010000200         1489 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.209, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000200 DALAGD010000000
VERSION     DALAGD010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.209"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            98..634
                     /gene="amaP"
                     /locus_tag="O2701_002792"
     CDS             98..634
                     /gene="amaP"
                     /locus_tag="O2701_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCZ4294645.1"
                     /translation="MKRLKNFILGLLIVVIVGFLLFMYIQDGRITEYQDYFLQFEWFQ
                     PLLISLAALLILIGLILVFSIFKPTHRKPGLYKDFDDGHVYVSRKAVEKTAFDTVAKY
                     DQVRQPNVVAKLYNKKNKSYIDIKTDFFVPNNVQVQSLTEAIRSDIKKNVEYFTEMPV
                     RKLEVNVRDQKTSGPRVL"
     gene            647..886
                     /locus_tag="O2701_002793"
     CDS             647..886
                     /locus_tag="O2701_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCZ4294646.1"
                     /translation="MANNHNQNGQDSTQQVINFLKVFKWRIVGFLAFLLIAILFLTLG
                     FWKTVLIIVLCLIGVGIGYMKDRKQDFMNFLNRWS"
     gene            949..1458
                     /locus_tag="O2701_002794"
     CDS             949..1458
                     /locus_tag="O2701_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCZ4294647.1"
                     /translation="MTVDNNKAKQAYDNQTGVNEKEREERQKQQEQNQEPQFKNKLTF
                     SDEVVEKIAGIAAREVKGILDMKGGLTDTFTNAFSSGNNVTQGVSVEVGEKQAAVDLK
                     VILEYGESAPKIFRKVTELVKEQVKYITGLDVVEVNMQVDDVMTQKEWKQKHEKNNEN
                     NNQERQGLQ"
BASE COUNT          573 a          200 c          264 g          452 t
ORIGIN      
        1 tttaaagtta ttaatttatt atgtttaaga aaaaaactga tgggtacata tttaatatgt
       61 ttttaaatta actttaacta aggaggtgtc gttaggggtg aaacgactta aaaactttat
      121 ccttggctta ttaatagtag taatcgtggg gttcctgtta ttcatgtata tccaagatgg
      181 acgtataact gaatatcaag actacttcct tcaatttgaa tggttccaac cattacttat
      241 ttcacttgct gcactattga tattaatagg tcttatatta gtatttagta tcttcaaacc
      301 tacgcatcga aaacctggat tatacaaaga cttcgatgat ggtcatgttt acgtatctcg
      361 taaagctgtt gaaaagacgg cattcgatac ggttgcaaaa tatgatcaag taagacaacc
      421 aaatgtagtt gcgaaactat ataacaaaaa aaataaatct tatatcgaca ttaaaactga
      481 ctttttcgta ccaaataatg ttcaagttca atctttaact gaagcaattc gttcggatat
      541 taaaaagaat gtagaatatt tcacagaaat gccagtacgt aagttagaag ttaatgtgag
      601 agatcagaaa acttctggtc cacgagtgtt gtaagggagg tatatcatgg ctaacaatca
      661 taaccaaaac ggacaagact ctacacaaca agtgattaat tttttaaaag tatttaaatg
      721 gagaatcgtt ggtttcctag cgtttctatt aatcgcgata ttattcttaa cgttaggttt
      781 ttggaaaaca gtacttatca tcgttttatg tttaattggt gtaggtattg ggtatatgaa
      841 agaccgtaag caagatttta tgaatttttt aaatcgatgg agttaattca tcaatttaga
      901 atttaattaa aaaaatataa aaattaacaa gtaaaaggag aatctattat gactgtagat
      961 aacaataaag caaaacaagc atacgacaat caaactggtg ttaacgaaaa agaaagagaa
     1021 gagcgtcaaa aacaacaaga acaaaatcag gagcctcaat tcaaaaacaa attaacattc
     1081 tctgatgaag ttgttgaaaa aattgctggt atcgctgcac gtgaagttaa aggtatctta
     1141 gatatgaaag gtggcttaac tgatacattc actaatgcat tctcaagtgg caacaatgtt
     1201 actcaaggtg tatctgttga agttggtgaa aaacaagctg ctgtagactt aaaagtaatc
     1261 ttagaatatg gtgaatcagc accaaaaatc ttccgtaaag taactgaatt agttaaagaa
     1321 caagttaaat atattactgg tttagatgta gttgaagtta acatgcaagt tgacgatgta
     1381 atgactcaaa aagagtggaa acaaaaacac gaaaaaaata acgaaaacaa taaccaagaa
     1441 agacaaggtt tacaataagt tattcgataa aaagggcttg ggacattaa
//
LOCUS       DALAGD010000201         1470 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.210, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000201 DALAGD010000000
VERSION     DALAGD010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.210"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            249..1208
                     /gene="hyl"
                     /locus_tag="O2701_002795"
     CDS             249..1208
                     /gene="hyl"
                     /locus_tag="O2701_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hemolysin"
                     /protein_id="HCZ4294648.1"
                     /translation="MKTRIVSSVTTTLLLGSILMNPVANAADSDINIKTGTTDIGSNT
                     TVKTGDLVTYDKENGMHKKVFYSFIDDKNHNKKLLVIRTKGTIAGQYRVYSEEGANKS
                     GLAWPSAFKVQLQLPDNEVAQISDYYPRNSIDTKEYMSTLTYGFNGNVTGDDTGKIGG
                     LIGANVSIGHTLKYVQPDFKTILESPTDKKVGWKVIFNNMVNQNWGPYDRDSWNPVYG
                     NQLFMKTRNGSMKAADNFLDPNKASSLLSSGFSPDFATVITMDRKASKQQTNIDVIYE
                     RVRDDYQLHWTSTNWKGTNTKDKWIDRSSERYKIDWEKEEMTN"
BASE COUNT          563 a          215 c          227 g          465 t
ORIGIN      
        1 cataaagaat ttattcaact ttgactaacc ctcgaaattg aaatgcttcc tttcaaattt
       61 taaataaaag ttaaaaacat attcttaaaa taatcactca tcatcactca gtaatttatc
      121 agttgctaca ttaaatatac taatataaac gattataaat atttgatatg tctcaactgc
      181 aatattctaa attgacatat tgattattgt ttcctcaaat cgttttaaaa aatagaagga
      241 tgatgaaaat gaaaacacgt atagtcagct cagtaacaac aacactattg ctaggttcca
      301 tattaatgaa tcctgtcgct aatgccgcag attctgatat taatattaaa accggtacta
      361 cagatattgg aagcaatact acagtaaaaa caggtgattt agtcacttat gataaagaaa
      421 atggcatgca caaaaaagta ttttatagtt ttatcgatga taaaaatcat aataaaaaac
      481 tgctagttat tagaacgaaa ggtaccattg ctggtcaata tagagtttat agcgaagaag
      541 gtgctaacaa aagtggttta gcctggcctt cagcctttaa ggtacagttg caactacctg
      601 ataatgaagt agctcaaata tctgattact atccaagaaa ttcgattgat acaaaagagt
      661 atatgagtac tttaacttat ggattcaacg gtaatgttac tggtgatgat acaggaaaaa
      721 ttggcggcct tattggtgca aatgtttcga ttggtcatac actgaaatat gttcaacctg
      781 atttcaaaac aattttagag agcccaactg ataaaaaagt aggctggaaa gtgatattta
      841 acaatatggt gaatcaaaat tggggaccat atgatagaga ttcttggaac ccggtatatg
      901 gcaatcaact tttcatgaaa actagaaatg gctctatgaa agcagcagat aacttccttg
      961 atcctaacaa agcaagttct ctattatctt cagggttttc accagacttc gctacagtta
     1021 ttactatgga tagaaaagca tccaaacaac aaacaaatat agatgtaata tacgaacgag
     1081 ttcgtgatga ctaccaattg cactggactt caacaaattg gaaaggtacc aatactaaag
     1141 ataaatggat agatcgttct tcagaaagat ataaaatcga ttgggaaaaa gaagaaatga
     1201 caaattaatg taaattattt gttcatgtac aaataaatat aatttataac tttagccgat
     1261 aacttcagaa atgatgtttt tcggctaatt tttatactga atttagttta attaacttat
     1321 gatattttta atttaaacta tctacttgat ttgctttcct gacttaaaaa ttagactaaa
     1381 catgacacta tatgaggtaa tttttaaaat aagaaatttg aattccatgt tgtcaaaccc
     1441 tttatataaa agttttgaaa ttgttcaaaa
//
LOCUS       DALAGD010000202         1470 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.211, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000202 DALAGD010000000
VERSION     DALAGD010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.211"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            67..1413
                     /locus_tag="O2701_002796"
     CDS             67..1413
                     /locus_tag="O2701_002796"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCZ4294649.1"
                     /translation="MHFIAISINHRTADVALREQVAFRDDALRIAHEDLYETKSILEN
                     VILSTCNRTEVYAVVDQIHTGRYYIQRFLARAFGFEVDDIKAMSEVKVGDEAVEHLLR
                     VTSGLDSIVLGETQILGQIRDAFFLAQSTGTTGTIFNHLFKQAITFAKRAHNETDIAD
                     NAVSVSYAAVELAKKVFGKLKSKQAIIIGAGEMSELSLLNLLGSGITDITVVNRTIEN
                     AMKLAAKHQVKYDELSSLPNLLESADIVISSTSAQSYIITNEMIERIAENRKQDSLVL
                     IDIAVPRDIEPGISAITNIFNYDVDDLKGLVDANLRERQLAAATISEQIPAEIHAHNE
                     WISMLGVVPVIRALREKAMAIQAETMDSIDRKLPGLSERERKIISKHTKSIINQMLKD
                     PIKQAKELSSDKKSNEKLELFQNIFDIEAECPHEQAKQQKESKVKEISARRIFSFE"
BASE COUNT          538 a          197 c          289 g          446 t
ORIGIN      
        1 gtacttaatt ggattttaaa ttatgttata atatttgtat tgttagtata tatgggggcg
       61 tttcaaatgc attttattgc aattagtata aatcatcgca cagctgatgt tgcactaaga
      121 gagcaagttg cttttagaga tgatgcctta cgaattgccc atgaagattt atatgaaact
      181 aaatctattt tagaaaatgt catattatca acatgtaatc gaactgaagt atatgctgtt
      241 gttgatcaaa ttcacacagg tcgttactat attcaacgat ttctagctcg tgcatttgga
      301 tttgaagtag atgatattaa agcaatgtca gaagtaaaag tgggggacga agcagtagaa
      361 catttattgc gtgtcacttc tggtttagat tcaatcgtac ttggagaaac tcaaatttta
      421 ggtcaaataa gagatgcatt tttcttagcg caaagcacag gtacgacagg aacaattttt
      481 aatcatctat ttaaacaggc aattactttt gcaaaaagag cacataatga aacagatata
      541 gctgataatg ctgtaagtgt gtcttatgct gcggtcgagt tggcgaaaaa agtatttggc
      601 aaattgaaaa gtaagcaagc tatcattatt ggtgcagggg aaatgagtga attatcacta
      661 ttaaatcttc ttggttctgg aattactgat attacagtag taaatagaac aattgaaaat
      721 gctatgaaat tagcagcaaa gcatcaagtg aaatatgatg aactatcatc attaccaaat
      781 ttacttgaaa gtgcagatat tgtgattagt tcaacgagtg cacaatctta tatcattaca
      841 aatgaaatga tagaaagaat tgcagaaaat agaaagcaag attcactagt attgattgat
      901 attgcagttc ctcgagatat tgaacctggt attagtgcca tcacaaacat ctttaattat
      961 gatgttgatg acttaaaagg tttagttgat gcaaacttac gtgagcgaca attagcggct
     1021 gcaacaattt cggaacaaat tcctgcagaa atacatgcac acaatgagtg gattagtatg
     1081 ttgggtgttg ttccagtgat tagagcttta cgtgaaaaag ctatggcaat tcaagcagaa
     1141 acgatggaca gtattgatcg taaattgcca gggttaagcg aaagagaacg taaaattata
     1201 tctaaacata caaaaagtat catcaatcaa atgttgaaag atcctattaa acaggccaaa
     1261 gaattaagta gtgataagaa aagtaatgaa aaattagagc tatttcaaaa tatatttgat
     1321 atcgaagctg aatgtcctca tgagcaagcg aagcaacaaa aagaaagcaa ggtcaaggaa
     1381 atttcagcgc gacgtatctt tagttttgaa taagcatatt aatatggtga taatatgcaa
     1441 gaaaacctgt ttattcgatt caatgaaatt
//
LOCUS       DALAGD010000203         1450 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.212, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000203 DALAGD010000000
VERSION     DALAGD010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.212"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            372..1433
                     /locus_tag="O2701_002797"
     CDS             372..1433
                     /locus_tag="O2701_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HCZ4294650.1"
                     /translation="MSNANNADNKQFFSKILNSIGAGVVIALVPNALLGEVLKIFKSG
                     NEILELTYQLVILIQSFMAFIIGVLAAHQFKFNGAGAAIVGTSAMIGSGAVVYSNNSF
                     MLKGIGDIINTSLVVIIACLIYMVLQNKLGSFELIILPVLVPIVSGGIGLITLPYIRK
                     ITQAIGNVIHSFTDLNPLLMSILISVAFSLLMVTPISLVAIATAISLNGLGSGAANLG
                     IVAACVTFLFGSLRVNSIGVNAVLLIGAAKMMIPVYLKNLIISIPLTINGIITGIIAY
                     VLQVKGTPLSAGFGYTGLVGPINAFNRMSGDPTMNIILLALGYFVIPFVSAFIVHELC
                     KKFIPIYSNDIYKFEVPKQ"
BASE COUNT          453 a          172 c          245 g          580 t
ORIGIN      
        1 tttaacttct ttttttggta ttacattaat gataatgatt atattatctg gggttatata
       61 tttgtttcaa cttttctaat aatatatttg taaaagtgct attttattgt gctttatacc
      121 aagtcgattc aatttatcta tcttaatagt aatatggcta ttttaatatt tacaatttat
      181 tacattatta gtcacaggat gtaattagta gtattttaaa attaacatga cattgattag
      241 tgagtagatt gtttaaattt gctttattca atttgtggat tgttatattt agattaaatt
      301 agccaactaa tcattgttaa agttaatttt ctataatatg atgagtttaa tgttcaaatt
      361 ggaggtcgtt tatgagtaac gcaaataatg ctgataataa acagtttttc agtaagattt
      421 taaattctat tggtgctggt gttgttattg cacttgttcc gaatgcttta ttaggtgaag
      481 tattaaaaat atttaaaagt ggtaatgaaa ttttagaatt aacgtatcag ttagtgatac
      541 taattcaatc atttatggca tttattattg gggttttagc tgctcatcaa tttaaattta
      601 atggtgcagg tgctgctata gtaggtacat cagctatgat tggttcaggt gctgttgtat
      661 atagcaataa tagttttatg ttaaaaggta taggtgacat tataaatacg agtttggttg
      721 ttattattgc atgtcttata tatatggtat tacaaaacaa acttggatct tttgaattaa
      781 ttatattacc agttttagta cctatcgtta gtgggggtat cggtctaatc acattacctt
      841 acattcgaaa aattactcaa gcaattggaa atgtaattca ttcttttaca gacttaaatc
      901 cgttgttaat gtctatactt attagtgtgg cattttcatt attaatggta acaccaatat
      961 cgttagtagc gattgcaact gcgattagtt taaacggact aggaagtgga gcagcaaacc
     1021 taggtatagt ggctgcctgt gttactttct tatttggctc attgcgtgtc aattctattg
     1081 gtgtaaatgc agttttactc ataggagctg caaaaatgat gatcccagtt tacttgaaaa
     1141 atttaattat ttcgatacca ttgactataa atggaataat tactggaatc attgcttacg
     1201 ttcttcaggt taaaggaaca ccattgtctg ctggatttgg atatacagga ttagttggcc
     1261 caataaatgc atttaaccga atgtcaggtg atccaacaat gaatattatt ttattagcac
     1321 ttggttattt tgtcatacca tttgtatcgg catttattgt tcatgaattg tgtaaaaaat
     1381 tcattcctat atattctaat gatatttata aatttgaagt tccaaaacaa taatttatag
     1441 cttgatgaca
//
LOCUS       DALAGD010000204         1413 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.213, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000204 DALAGD010000000
VERSION     DALAGD010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.213"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            1..551
                     /locus_tag="O2701_002798"
                     /pseudo
     CDS             1..551
                     /locus_tag="O2701_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500456.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1829 domain-containing protein"
     gene            1012..1220
                     /locus_tag="O2701_002799"
                     /pseudo
     CDS             1012..1220
                     /locus_tag="O2701_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          569 a          197 c          185 g          462 t
ORIGIN      
        1 tcaaaaaaac gtaaaaagat ttttgaagag cacctttcag cttacggtat taaatataac
       61 gataaaactc acgaaatttt tgttcaaact aactttaaaa attttaataa atcgaaacat
      121 aatttattac agtgccttat atttgttaat gatatgtact tactttctaa tcctaagtca
      181 cagaacatat ttacagaaga tgttgcaaac aaattggatg aacataacat ttattacgga
      241 agagatttac ctattatagg aagcagtggt gttgttcata atttcgactt ttttattagc
      301 gctaagaaaa atcaaaaaga aaaatttatc aatgctattt ctaaccctaa taattctatg
      361 attattaagt cgaaaataac ggatgctatg caagcaaaaa aataaaaaga cacaggcaaa
      421 atgagtttat ttttatttta aatgactcaa aaaaagaaat aaatgaacat aataaaaatc
      481 ttcttcatga aaactatatt agtacaatag attatagcga attagatgaa aagataggtt
      541 tattgattta atatatatag acgtgataat atcaatgttt atattaaatg aaacgaattt
      601 gaaaatttcg aaacagctta agatagcaaa ttgaatagcc ttattgataa tgcagaatcg
      661 tctacactta gttgaacaaa ttctatgaga atagatattg ttaaactatt tgggtaggcg
      721 attttatttt gacaagagtc agaagattat ttagttcata gtttaagtta caaatggcta
      781 aactttatga aaatggtatg actagaatcg aaattatact cgaatacgat ttaacactct
      841 caatcttctc gaactgaata aatactgggt cattcaatca tcaagacaac ttaataagtg
      901 atgataaatg ttcataccat tttgcaaata taaccagata ctctcaacaa aatgtagagt
      961 tcaaaattaa aaatgtccct aattggcaca ttgctgtatg ataataaatt caacttagat
     1021 ttcaacaata taactaatga tgcttatatt taccgcgata tcaaaccaaa cagcccatcc
     1081 acaaacaaat tagaatatat catgttaata atagtaaatg atatatcttc caaagaccgc
     1141 aaactgtagc agaacatgag aaccttgcga ttttgaaatg gtctaataaa tcaaatatca
     1201 ttgaaacttt aatcacataa attaacagta gtcacctgtt cgcagttcta taaattacaa
     1261 atgcaataaa ggctaaagta aactaattac tttagccttt attaataatt tagtcgaatt
     1321 tattaaaaat cgactaactc ctggttatgc tacgctttac tttataatcc gcacgtagat
     1381 gccgattgat tatattataa tattattatt tta
//
LOCUS       DALAGD010000205         1394 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.214, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000205 DALAGD010000000
VERSION     DALAGD010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.214"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            212..1066
                     /locus_tag="O2701_002800"
     CDS             212..1066
                     /locus_tag="O2701_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HCZ4294651.1"
                     /translation="MNKHKKGSIFGIIGLVVIFAVVSFLFFSMISDQIFFKHVKSDIK
                     IEKLNVTLNDAAKKQINNYTSQQVSNKKNDAWRDASATEIKSAMDSGTFIDNEKQKYQ
                     FLDLSKYQGIDKNRIKRMLVDRPTLLKHTDDFLKAAKDKHVNEVYLISHALLETGAVK
                     SELANGVEIDGKKYYNFYGVGALDKDPIKTGAEYAKKHGWDTPEKAISGGADFIHKHF
                     LSSTDQNTLYSMRWNPKNPGEHQYATDIKWAESNATIIADFYKNMKTEGKYFKYFVYK
                     DDSKHLNK"
BASE COUNT          529 a          183 c          248 g          434 t
ORIGIN      
        1 aaaggttata caaaaagata aaacatttat gattcgtaga tcaacgtaaa gtaatgttga
       61 taaatggttt aaaacgtttc atttacatta ctgtttattt atgaatatgt aacaatgcat
      121 agataaaatt gttaaacggt tttaataatg ttaaacttta ttatcgtagt caaactgaat
      181 gtataacaac aatgacctaa gaggtgtgga tatgaataaa cacaagaaag gttctatttt
      241 tggaataata ggacttgttg tcatatttgc tgttgtctca tttttatttt tctcaatgat
      301 atccgatcag atatttttca aacatgttaa atccgacatt aagattgaaa agttaaatgt
      361 tacattaaac gatgcagcaa agaaacaaat aaataattat acgagtcaac aggtatcaaa
      421 taaaaagaat gatgcatgga gagatgcatc tgcaactgaa attaaaagtg caatggatag
      481 cggtactttt atcgataatg aaaagcaaaa atatcaattt ttagatttat caaagtatca
      541 agggattgat aaaaatagaa ttaaacgtat gttagtagat agaccaacgt tattgaaaca
      601 tacggatgat ttcttaaaag ctgctaaaga taagcacgtt aacgaagttt atttaatttc
      661 acatgcatta ttagaaactg gcgcagttaa aagtgaatta gctaatggag tcgaaattga
      721 tggcaaaaag tactacaatt tctatggagt aggagccctt gataaagacc caattaaaac
      781 aggtgcagaa tatgctaaaa agcatggttg ggatacacct gaaaaagcta tttcaggcgg
      841 tgctgatttc attcataagc acttcttatc aagcacagat caaaatacat tgtatagtat
      901 gagatggaat ccaaaaaatc caggagaaca tcaatatgct acagatatta agtgggcaga
      961 aagtaatgca acaattatcg ctgactttta taagaacatg aagactgaag gaaaatactt
     1021 caaatacttt gtgtataaag atgacagtaa acatttgaat aagtaatttg ataagctacg
     1081 agttgttttt atgactcgga catactaaaa agacgctttc tatcttgttt tgatagaaag
     1141 cgtctttttg cattagagaa aacacattga ttgataatcc caccaatgca agtggggcag
     1201 gacatcgata aagaattact ttttctttag aaattagtat ttcttatgca tgagttttac
     1261 tcatgtattc ctatttttaa atacacatta gctgtggctt atgaaaacag gctgggacat
     1321 aaatcaatgt tctatgctct acgaagttat attggcagta gttgactgaa cgaaaatgcg
     1381 cttgtaacaa gctt
//
LOCUS       DALAGD010000206         1380 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.215, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000206 DALAGD010000000
VERSION     DALAGD010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.215"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(218..1309)
                     /locus_tag="O2701_002801"
     CDS             complement(218..1309)
                     /locus_tag="O2701_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase"
                     /protein_id="HCZ4294652.1"
                     /translation="MSNKLESYRSEIVSLNHQILDLLSKRGELAQKIGEEKLKQGTRI
                     YDPQREKEMLNDLIDSNKGPFNDNTIKQLFKEIFKASTDLQKSENEKHLYVSRKLKPE
                     DTIVTFDNGGIIGDGNKSFVFGPCSVESFEQVEAVAKNLHAKGEKFIRGGAFKPRTSP
                     YDFQGLGVEGLKILKQIKDKYDLNVVSEIVNPNDFEVADEYLDVFQIGARNMQNFELL
                     KEAGRTKKPILLKRGLSATIEEFVYAAEYIASQGNQNIILCERGIRTYEKATRNTLDI
                     SAVPILKQGTHLPVMVDVTHSTGRKDIMLPTAKAALAVGADGVMAEVHPDPSVALSDA
                     GQQMDLDEFQAFYDELKPLADLYNAKKLK"
BASE COUNT          432 a          248 c          194 g          506 t
ORIGIN      
        1 tcagtcataa tctttaataa ttgtgaaatt gaacaagtgt cgaagtatta taacaatcgt
       61 taagtgcagt atacctttat ttctatacaa aaaataatag ccactctaaa agtaatatac
      121 tcttagaatg gctgtaagtt taaaattgtc gattcaaaga taatattcta cttcaacatg
      181 acatattagt taagtagtct atagtttcct tggaatatta ttttaacttt ttagcgttat
      241 ataaatcagc taaaggcttt aattcatcat aaaatgcttg gaattcatct aaatccattt
      301 gttgacccgc atcactaagt gcaacagatg gatctggatg cacctcagcc ataactccat
      361 cagcaccaac tgctaatgct gctttcgcag ttggtaacat gatatcttta cgacctgtac
      421 tatgcgtaac atctaccatg actggtaagt gtgtaccttg ttttaaaatt ggtactgctg
      481 aaatatctaa agtgttacgt gtcgcctttt cataagttcg gattccacgt tcacataaaa
      541 taatgttttg attaccttgt gaagcaatgt attcagctgc ataaacaaac tcttcgattg
      601 tagcagataa accacgtttt aatagaatag gctttttcgt acggccagct tcttttaata
      661 actcgaagtt ttgcatatta cgtgcaccaa tttggaatac gtctaaatac tcatcagcca
      721 cttcaaaatc atttggattt acgatttcgc tgacaacatt taaatcatat ttatctttaa
      781 tctgtttaag tattttaagt ccttcaacac ctaggccttg gaaatcatat ggtgatgtac
      841 gtggtttaaa tgcaccgcca cgaataaatt tttcaccttt agcatgtaag tttttagcaa
      901 cagcttcaac ttgttcaaat gattcaactg aacatggccc aaatacaaat gatttattgc
      961 cgtctccaat aatgccccca ttatcaaatg ttacaatcgt atcttcaggt ttcaacttac
     1021 gtgatacata taaatgtttt tcattttcag atttttgtaa atctgtagag gctttgaaaa
     1081 tttctttaaa taattgctta atagtattat cgttgaatgg tcctttgtta ctatcgatta
     1141 agtcgttaag catttctttt tcacgttgtg gatcataaat acgtgtacct tgttttaatt
     1201 tttcttcccc aattttttgt gctagttcac cacgtttaga taataagtct aaaatttgat
     1261 gattcagtga tacaatctca cttctgtatg attctaattt attactcatc cttactcacc
     1321 tctactaatt atttcaagta tattccactt taaagcaata aaatataatg ttaaaaaata
//
LOCUS       DALAGD010000207         1374 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.216, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000207 DALAGD010000000
VERSION     DALAGD010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.216"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(24..>1374)
                     /locus_tag="O2701_002802"
     rRNA            complement(24..>1374)
                     /locus_tag="O2701_002802"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          302 a          398 c          303 g          371 t
ORIGIN      
        1 agatgttccg aatatatcct tagaaaggag gtgatccagc cgcaccttcc gatacggcta
       61 ccttgttacg acttcacccc aatcatttgt cccaccttcg acggctagct cctaaaaggt
      121 tactccaccg gcttcgggtg ttacaaactc tcgtggtgtg acgggcggtg tgtacaagac
      181 ccgggaacgt attcaccgta gcatgctgat ctacgattac tagcgattcc agcttcatgt
      241 agtcgagttg cagactacaa tccgaactga gaacaacttt atgggatttg cttgacctcg
      301 cggtttcgct gccctttgta ttgtccattg tagcacgtgt gtagcccaaa tcataagggg
      361 catgatgatt tgacgtcatc cccaccttcc tccggtttgt caccggcagt caacttagag
      421 tgcccaactt aatgatggca actaagctta agggttgcgc tcgttgcggg acttaaccca
      481 acatctcacg acacgagctg acgacaacca tgcaccacct gtcactttgt cccccgaagg
      541 ggaaggctct atctctagag ttgtcaaagg atgtcaagat ttggtaaggt tcttcgcgtt
      601 gcttcgaatt aaaccacatg ctccaccgct tgtgcgggtc cccgtcaatt cctttgagtt
      661 tcaaccttgc ggtcgtactc cccaggcgga gtgcttaatg cgttagctgc agcactaagg
      721 ggcggaaacc ccctaacact tagcactcat cgtttacggc gtggactacc agggtatcta
      781 atcctgtttg atccccacgc tttcgcacat cagcgtcagt tacagaccag aaagtcgcct
      841 tcgccactgg tgttcctcca tatctctgcg catttcaccg ctacacatgg aattccactt
      901 tcctcttctg cactcaagtt ttccagtttc caatgaccct ccacggttga gccgtgggct
      961 ttcacatcag acttaaaaaa ccgcctacgc gcgctttacg cccaataatt ccggataacg
     1021 cttgccacct acgtattacc gcggctgctg gcacgtagtt agccgtggct ttctgattag
     1081 gtaccgtcaa gatgtgcaca gttacttaca catatgttct tccctaataa cagagtttta
     1141 cgatccgaag accttcatca ctcacgcggc gttgctccgt caggctttcg cccattgcgg
     1201 aagattccct actgctgcct cccgtaggag tctggaccgt gtctcagttc cagtgtggcc
     1261 gatcaccctc tcaggtcggc tatgcatcgt tgccttggta agccgttacc ttaccaacta
     1321 gctaatgcag cgcggatcca tctataagtg acagcaagac cgtctttcac tttt
//
LOCUS       DALAGD010000208         1355 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.217, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000208 DALAGD010000000
VERSION     DALAGD010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.217"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..1318
                     /locus_tag="O2701_002803"
     rRNA            <1..1318
                     /locus_tag="O2701_002803"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          358 a          302 c          399 g          296 t
ORIGIN      
        1 cactgccgag aaaagcctct agatagaaaa taggtgcccg taccgcaaac cgacacaggt
       61 agtcaagatg agaattctaa ggtgagcgag cgaactctcg ttaaggaact cggcaaaatg
      121 accccgtaac ttcgggagaa ggggtgctct ttagggttaa cgcccagaag agccgcagtg
      181 aataggccca agcgactgtt tatcaaaaac acaggtctct gctaaaccgt aaggtgatgt
      241 ataggggctg acgcctgccc ggtgctggaa ggttaagagg agtggttagc ttctgcgaag
      301 ctacgaatcg aagccccagt aaacggcggc cgtaactata acggtcctaa ggtagcgaaa
      361 ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa cgatttgggc actgtctcaa
      421 cgagagactc ggtgaaatca tagtacctgt gaagatgcag gttacccgcg acaggacgga
      481 aagaccccgt ggagctttac tgtagcctga tattgaaatt cggcacagct tgtacaggat
      541 aggtaggagc ctttgaaacg tgagcgctag cttacgtgga ggcgctggtg ggatactacc
      601 ctagctgtgt tggctttcta acccgcacca cttatcgtgg tgggagacag tgtcaggcgg
      661 gcagtttgac tggggcggtc gcctcctaaa aggtaacgga ggcgctcaaa ggttccctca
      721 gaatggttgg aaatcattca tagagtgtaa aggcataagg gagcttgact gcgagaccta
      781 caagtcgagc agggtcgaaa gacggactta gtgatccggt ggttccgcat ggaagggcca
      841 tcgctcaacg gataaaagct accccgggga taacaggctt atctccccca agagttcaca
      901 tcgacgggga ggtttggcac ctcgatgtcg gctcatcgca tcctggggct gtagtcggtc
      961 ccaagggttg ggctgttcgc ccattaaagc ggtacgcgag ctgggttcag aacgtcgtga
     1021 gacagttcgg tccctatccg tcgtgggcgt aggaaatttg agaggagctg tccttagtac
     1081 gagaggaccg ggatggacat acctctggtg taccagttgt cgtgccaacg gcatagctgg
     1141 gtagctatgt gtggacggga taagtgctga aagcatctaa gcatgaagcc cccctcaaga
     1201 tgagatttcc caacttcggt tataagatcc ctcaaagatg atgaggttaa taggttcgag
     1261 gtggaagcat ggtgacatgt ggagctgacg aatactaatc gatcgaagac ttaatcaaaa
     1321 taaatgtttt gcgaagcaaa atcactttta cttac
//
LOCUS       DALAGD010000209         1235 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.218, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000209 DALAGD010000000
VERSION     DALAGD010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.218"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(<1..>1235)
                     /locus_tag="O2701_002804"
     rRNA            complement(<1..>1235)
                     /locus_tag="O2701_002804"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          281 a          356 c          238 g          360 t
ORIGIN      
        1 taaaacgatt ataggtggta caggaatatc aacctgttat ccatcgccta cgcctgtcgg
       61 cctcagctta ggacccgact aacccagagc ggacgagcct tcctctggaa accttagtca
      121 atcggtggac gggattctca cccgtctttc gctactcaca ccggcattct cacttctaag
      181 cgctccacat gtccttacga tcatgcttca acgcccttag aacgctctcc taccattgtc
      241 caaaggacaa tccacagctt cggtaatatg tttagccccg gtacattttc ggcgcagtgt
      301 cactcgacta gtgagctatt acgcactctt taaatgatgg ctgcttctaa gccaacatcc
      361 tagttgtctg ggcaacgcca catccttttc cacttaacat atattttggg accttagctg
      421 gtggtctggg ctgtttccct ttcgaacacg gaccttatca cccatgttct gactcccaag
      481 ttaaattaat tggcattcgg agtttgtctg aattcggtaa cccgagaggg gcccctcgtc
      541 caaacagtgc tctacctcca ataatcatca cttgaggcta gccctaaagc tatttcggag
      601 agaaccagct atctccaggt tcgattggaa tttctccgct accctcagtt catccgctca
      661 cttttcaacg taagtcggtt cggtcctcca ttcagtgtta cctgaacttc aacctgacca
      721 agggtagatc acctggtttc gggtctacga ccaaatacta aacgccctat tcagactcgc
      781 tttcgctacg gctccacatt tactgcttaa ccttgcatca aatcgtaact cgccggttca
      841 ttctacaaaa ggcacgccat cacccattaa cgggctctga ctacttgtaa gcacacggtt
      901 tcaggttcta tttcactccc cttccggggt gcttttcacc tttccctcac ggtactggtt
      961 cactatcggt cactagagag tatttagcct taggagatgg tcctcccaga ttccgacgga
     1021 atttcacgtg ttccgtcgta ctcaggatcc actcaagaga gacaacattt tcgactacag
     1081 gattattacc ttctttgatt catctttcca gatgattcgt ctaatgtcgt cctttgtaac
     1141 tccgtataga gtgtcctaca accccaacaa gcaagcttgt tggtttgggc tcttcccgtt
     1201 tcgctcgccg ctactaaggg aatcgaattt tcttt
//
LOCUS       DALAGD010000210         1195 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.219, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000210 DALAGD010000000
VERSION     DALAGD010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.219"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(38..367)
                     /locus_tag="O2701_002805"
     CDS             complement(38..367)
                     /locus_tag="O2701_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294653.1"
                     /translation="MINETKYMRQQITNLIQIIDTIKYNTLMTDWNIQTHIYKFNQIV
                     TNELNKFKGFETSYIINENQVSYYEIITLLNKRPLRQVDYGNKIQYLNFYHTELSNAL
                     FAIKFAH"
     gene            416..1153
                     /gene="sdpB"
                     /locus_tag="O2701_002806"
     CDS             416..1153
                     /gene="sdpB"
                     /locus_tag="O2701_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpB"
                     /protein_id="HCZ4294654.1"
                     /translation="MENEKKKYTFKDIAWRDLLIIPIIGVIFYALTIAAYIGPILFGY
                     YEIDSNLIFVATITQALAYTIGIGAFYLFHLKVMPSRLRAGFAYIKKYWLRLSITYII
                     ALILIYTYEYMTQFLPKHLQYSETANELELNKMFEVPAFLPVAFLLIVIVGPIVEEIV
                     FRHILIGELGKKFNFIAMSIVSVFLFAFIHVTDAKSPFEFGPYLILSIILVFTYLKSG
                     RNLGSTIALHIANNFVSFVISVISIYA"
BASE COUNT          402 a          144 c          199 g          450 t
ORIGIN      
        1 aaataacttg tttaaatgac attttatgaa aaataggtta atgggcaaat ttaattgcaa
       61 ataatgcgtt agatagttct gtatgataaa aattaagata ttgaattttg ttaccatagt
      121 cgacttgtcg gaggggacgt ttattaagta gtgttataat ttcataatag gaaacttgat
      181 tttcgtttat tatatatgat gtttcaaagc ctttgaactt attcaattca ttagtaacta
      241 tttggttaaa tttataaata tgcgtttgaa tgttccaatc tgtcattaaa gtattatatt
      301 taatcgtatc aatgatttga atcaaattag taatttgttg tctcatatac ttagtttcgt
      361 ttatcatgta gaagtacctc caaaaatcaa ttattaaaca ggaaaggata taacagtgga
      421 aaatgaaaaa aagaaataca cgtttaaaga tatagcatgg agagatttgc ttataattcc
      481 aattataggt gttatatttt atgcactcac aattgcagct tacataggac caattttgtt
      541 tggttattat gaaatcgata gtaatctgat atttgtagcg actatcacac aagctttagc
      601 atatacgatt gggattggcg cattttattt atttcattta aaagtgatgc catcaaggct
      661 tcgagctggt tttgcgtata tcaaaaaata ttggcttaga ttgtcaataa cgtatattat
      721 tgctctcatc ttaatttata catatgaata tatgacacag tttctaccta aacatttgca
      781 atatagtgaa acagccaatg aacttgagct aaataaaatg tttgaagtgc ctgcattttt
      841 acctgtagca ttcttattaa ttgtgattgt aggtccaata gttgaagaaa tcgtatttag
      901 acatatttta attggagaat taggtaagaa gtttaatttt atagctatga gtattgtttc
      961 ggtgttctta tttgcattta ttcatgtgac tgatgcaaag tcgccgtttg agtttggtcc
     1021 atatctcata ctatccatca tattagtttt cacttattta aaatctggaa gaaacctagg
     1081 ttcaacaatt gcattacata tagcaaataa ttttgtttct ttcgttatat cagtaataag
     1141 tatttatgcg tagataaaga ggtcgggaca ttaagttttt aggcaatgaa aaaag
//
LOCUS       DALAGD010000211         1184 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.220, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000211 DALAGD010000000
VERSION     DALAGD010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.220"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            468..>1184
                     /locus_tag="O2701_002807"
     CDS             468..>1184
                     /locus_tag="O2701_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HCZ4294655.1"
                     /translation="MGEFEVKYLTGFILILLLEGIFTNSASAIEYSDLHHKSKFDSKR
                     LSNAKMSFINPTQLENKNTNDRLLKHDLLFHDMFVNDDWKKDFKVEFENEALSKKFIN
                     KDIDIFAGNYGYGCHGGATNKTQCSYGGVTLSDNNKYDDYKNIPCNLWIDGHQTEIEL
                     TAVKTKKKIVTIQELEVQLRNYLNEKYKLYEQGGDIVKGYVKYYNDDEQNVEYDFYNL
                     NGEYGREVLKMYADNKTINSD"
BASE COUNT          454 a          118 c          212 g          400 t
ORIGIN      
        1 agaccaacaa tttatgagat aagtgatgaa tttctaaatt cgattataaa ataaatataa
       61 tatgtgatca gctatactta tagtatttgt aagatcgcgt aattgcttca ttgaaaaaat
      121 atatttgttg agagtggtgc atgacaaatg tactgctctt tttgttgtgt atcagtattg
      181 tgatgtttta atgagaatat tatatgaatc attatgaaat ttaataaaaa taaaagaaat
      241 gattatcatt ttttcttata tactgttaaa cggtttggaa tttttaggta tacactgtat
      301 tggttgatat aactcaacta ataattgcga acagagtatt tcaaattgaa aagtattatg
      361 agcgtgatac ataatcaaaa ttgtaggctc aagaaccact acataataaa ccataagcgg
      421 ttctttatca tttatgtctc gctctcaaat gtaaattaat aattgttttg ggggagtttg
      481 aagttaaata tttaacagga tttattttaa tattattgtt agaaggaatt tttacaaatt
      541 cagcgagtgc aatcgaatat tcagacttac atcataaaag taagtttgat tcaaagcgtc
      601 taagtaatgc taagatgtca ttcatcaatc caactcagct tgaaaataaa aacacaaacg
      661 atagactgtt gaagcatgat ttgttatttc atgacatgtt cgtaaatgat gattggaaaa
      721 aggattttaa agttgaattt gaaaatgagg cactttcaaa gaaatttata aataaggata
      781 tcgatatatt tgctggaaat tatggatacg gatgtcatgg gggagcaacc aataaaacgc
      841 aatgtagtta tggtggtgtt actttaagtg acaataataa atacgatgat tataagaata
      901 taccttgtaa tttatggatt gacggacatc aaacagaaat agaactaact gcagtaaaaa
      961 cgaaaaagaa aattgttact attcaagaat tagaggttca attaagaaat tatttgaatg
     1021 agaagtataa gttgtacgaa caaggtggcg acattgttaa agggtatgtt aaatattata
     1081 atgatgatga acaaaatgta gaatatgatt tttataattt aaatggtgag tatggtcgtg
     1141 aggtattaaa aatgtatgct gataataaaa ctatcaatag tgac
//
LOCUS       DALAGD010000212         1175 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.221, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000212 DALAGD010000000
VERSION     DALAGD010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.221"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            87..656
                     /gene="agrB"
                     /locus_tag="O2701_002808"
     CDS             87..656
                     /gene="agrB"
                     /locus_tag="O2701_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator AgrB"
                     /protein_id="HCZ4294656.1"
                     /translation="MNYFDNKIDQFATYLQKRNNLDHIQFLQVRLGMQVLAKNIGKLI
                     VMYTIAYILNIFLFTLITNLTFYLIRRHAHGAHAPSSFWCYVESIILFILLPLVIVNF
                     HINFLIMIILTVISLGVISVYAPAATKKKPIPVRLIKRKKYYAIIVSLTLFIITLIIK
                     EPFAQFIQLGIIIEAITLLPIFFIKEDLK"
     gene            653..793
                     /locus_tag="O2701_002809"
     CDS             653..793
                     /locus_tag="O2701_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HCZ4294657.1"
                     /translation="MNTLFNLFFDFITGILKNIGNIAAYSTCDFIMDEVEVPKELTQL
                     HE"
BASE COUNT          399 a          148 c          160 g          468 t
ORIGIN      
        1 taagtattta tttcctacag ttaggcaata taatgataaa agattgtact aaatcgtata
       61 atgacagtga ggagagtggt gtaaaattga attattttga taataaaatt gaccagtttg
      121 ccacgtatct tcaaaagaga aataacttag atcatattca atttttgcaa gtacgattag
      181 ggatgcaggt cttagctaaa aatataggta aattaattgt tatgtatact attgcctata
      241 ttttaaacat ttttctgttt acgttaatta cgaatttaac attttattta ataagaagac
      301 atgcacatgg tgcacatgca ccttcttctt tttggtgtta tgtagaaagt attatactat
      361 ttatactttt acctttagta atagtaaatt ttcatattaa ctttttaatt atgattattt
      421 taacagttat ttctttaggt gtaatctcag tatatgctcc tgcagcaact aaaaagaagc
      481 ccattcctgt gcgacttatt aaacgaaaaa aatattatgc gattattgtt agtttaaccc
      541 ttttcattat cacacttatc atcaaagagc catttgccca attcattcaa ttaggcatca
      601 taatagaagc tattacatta ttacctattt tctttattaa ggaggactta aaatgaatac
      661 attatttaac ttattttttg attttattac tgggatttta aaaaacattg gtaacatcgc
      721 agcttatagt acttgtgact tcataatgga tgaagttgaa gtaccaaaag aattaacaca
      781 attacacgaa taatttaaat agagagtgtg atagtaggtg gaattattaa atagttataa
      841 ttttgtttta ttcgtattaa ctcaaatgat attaatgttt acaataccag ctataattag
      901 tggtattaag tacagtaaac ttgattattt tttcatcata gtaatttcga cattatcgtt
      961 atttctattt aaaatgtttg atagcgcgtc cttaatcata ttaacttcat ttattattat
     1021 aatgtatttt gtcaaaatca aatggtattc tattttgttg attatgactt cgcagattat
     1081 tctatactgt gctaactaca tgtatatagt tatatatgca tatatcacca aaatttctga
     1141 tagtatattt gtaatattcc ctagcttttt tgtag
//
LOCUS       DALAGD010000213         1166 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.222, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000213 DALAGD010000000
VERSION     DALAGD010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.222"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            65..415
                     /gene="scn"
                     /locus_tag="O2701_002810"
     CDS             65..415
                     /gene="scn"
                     /locus_tag="O2701_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN-A"
                     /protein_id="HCZ4294658.1"
                     /translation="MKIRKSILAGTLAIVLASPLVTNLDKNEAQASTSLPTSNEYQNE
                     KLANELKSLLDELNVNELATGSLNTYYKRTIKISGQKAMYALKSKDFKKMSEAKYQLQ
                     KIYNEIDEALKSKY"
     gene            complement(468..728)
                     /locus_tag="O2701_002811"
     CDS             complement(468..728)
                     /locus_tag="O2701_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294659.1"
                     /translation="MCIVIYLIYSLIVYFICIINFFIMAAPFLMTNADYVWTPMTTVT
                     LFILSLIIFLIFIKTKDVVHLTIFILNLLFSILYCLPILFYL"
     gene            complement(1039..>1166)
                     /locus_tag="O2701_002812"
     CDS             complement(1039..>1166)
                     /locus_tag="O2701_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294660.1"
                     /translation="QEINNRESEKGCLWGISMLILLFLLILFGITACSSSIHFIN"
BASE COUNT          513 a          157 c          153 g          343 t
ORIGIN      
        1 taatgtactt ttttttagtc agtcattaaa ataaattagt actaattact aaggagaata
       61 aaaaatgaaa attagaaaat ctatacttgc gggaacttta gcaatcgttt tagcatcacc
      121 actagtaact aatctagata aaaatgaggc acaagctagc acaagcttgc caacatcgaa
      181 tgaatatcaa aacgaaaagt tagctaatga attaaaatcg ttattagatg aactaaatgt
      241 taatgaatta gctactggaa gtttaaacac ttattataag cgaactataa aaatttcagg
      301 tcaaaaagca atgtatgctc ttaagtcaaa agactttaag aaaatgtcag aagcaaaata
      361 tcaacttcaa aagatttata acgaaattga cgaagcacta aaaagtaaat attaaaaaaa
      421 ccaccctttt acgggtggtt ttaattttct agataatata aaagtgttca taaataaaac
      481 agtataggca aacaataaag tattgaaaaa agtaagttta atatgaaaat tgttaaatga
      541 acgacatctt ttgtttttat aaatatcaag aaaataatca aactcaaaat aaataacgta
      601 actgtagtca taggcgtcca tacataatca gcattagtca ttaagaatgg tgcagccatt
      661 atgaaaaaat ttataatgca gatgaaatag acaattagac tataaattag ataaataaca
      721 atacacaccc ttcataaata aataatttaa atcctatata ttttaacaaa agtaacacac
      781 agaagtgtag aaaataaaaa atattggtaa ataaaatcaa taagtttaac caatatgttg
      841 ctcgcttcat accgtatatt gcaacaaaaa ttccgattaa gaaaaatata gcccctatga
      901 taaaacagaa atccgatgct gaattattaa aaaatgaggt gtttagagtt agaaaatgag
      961 ttaatgagtt gactataact aataagatat taattatatt tgtatggttc ttcacatgat
     1021 acctccaagt aaaaaaatct aattaataaa gtgaatgctt gatgaacaag cagttattcc
     1081 aaacagaatc aataagaaaa gtagaatcaa catgctaatg ccccataaac aacccttttc
     1141 actttctcta ttattaattt cttgac
//
LOCUS       DALAGD010000214         1128 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.223, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000214 DALAGD010000000
VERSION     DALAGD010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.223"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..200
                     /gene="splF"
                     /locus_tag="O2701_002813"
     CDS             <1..200
                     /gene="splF"
                     /locus_tag="O2701_002813"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease SplF"
                     /protein_id="HCZ4294661.1"
                     /translation="SVNGNIVSSDAIIQPGSSGSPILNSKHEAIGVIYAGNKPSGEST
                     RGFAVYFSPEIKKFIADNLDK"
     gene            265..399
                     /locus_tag="O2701_002814"
     CDS             265..399
                     /locus_tag="O2701_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294662.1"
                     /translation="MHYLICIKANQRLIFTNSIVDWFYLCMNEQLFDIIKNINDFESI
                     "
     gene            563..>1128
                     /locus_tag="O2701_002815"
     CDS             563..>1128
                     /locus_tag="O2701_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HCZ4294663.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     RFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIR
                     EIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALIS
                     KVMVNLDDLPFVHSDMEIDMLGDAYEFL"
BASE COUNT          409 a          185 c          195 g          339 t
ORIGIN      
        1 tatcagtgaa tgggaatata gtgtcatcgg atgcaattat tcagcctggt agctctggtt
       61 cacctatatt aaatagtaaa cacgaagcta ttggtgtaat ctatgccggt aataagccat
      121 caggtgaaag cacaagagga tttgctgttt atttctctcc tgaaattaag aaattcattg
      181 cagataattt agataaataa ttaaaactta gacattcacc caatcctgac aaaatatact
      241 ataactaaca tttattaata tatattgcat tatttaatat gcatcaaagc caatcaacga
      301 ttgattttca ccaactcaat tgttgattgg ttttatttat gtatgaatga acaacttttt
      361 gacatcatta agaatataaa tgattttgaa agcatttgaa agctacaaca tttctataaa
      421 atttttcaat aacaattgcg ccactaaaac tcaaaatttc caccaccaac atccaaatta
      481 tcaacatcgc aacataacca aatgttataa taaatctatt acacaaagag ataaattact
      541 tatgcaaagg cggaggaatc acatgtctat tactgaaaaa caacgtcagc aacaagctga
      601 attacataaa aaattatggt cgattgcgaa tgatttaaga gggaacatgg atgcgagtga
      661 attccgtaat tacattttag gcttgatttt ctatcgcttc ttatctgaaa aagccgaaca
      721 agaatatgca gatgccttgt caggtgaaga catcacgtat caagaagcat gggcagatga
      781 agaatatcgt gaagacttaa aagcagaatt aattgatcaa gtcggttact tcattgagcc
      841 acaagattta ttcagtgcga tgattcgtga aattgaaacg caagatttcg atatagaaca
      901 tctagcgacg gcgattcgca aagttgaaac atctacatta ggtgaagaaa gtgaaaatga
      961 ctttatcggg ctgttcagcg atatggattt gagttcaacg cgactaggta acaatgtcaa
     1021 agaacgtact gctttaatct ctaaagtcat ggttaatctt gacgacttac cattcgttca
     1081 cagtgacatg gaaattgata tgttaggtga tgcatatgaa ttcctaat
//
LOCUS       DALAGD010000215         1108 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.224, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000215 DALAGD010000000
VERSION     DALAGD010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.224"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            8..976
                     /locus_tag="O2701_002816"
     CDS             8..976
                     /locus_tag="O2701_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCZ4294664.1"
                     /translation="MLLNLLLIFILIVICPLIFNYTLTLNLTCYILVVISIFLSLIYS
                     IMTYTFPWSMFVTFIIISIIMLFKHRYLFSHTSSRLFIKKLLSFSYKFVLYTSALLFI
                     STIQSPIVKGLSMWLAILCLSLLLTFICYLVWTVSYRHRSYNKEVDIIIVLGAGIFTE
                     FVTPMLAARLDRALDIYQQQASATKIIVSGGQGPDEPISEALAMQRYLIAHGVDKTDI
                     LMESHSINTYTNFLYSKQIINNLYHEAVKIVCVTSQFHVLRALRLAQKLGLSIDGVGS
                     HTPYHFFLHVLIKDYLGVMYQYRLLLTLYCSSSWFICLYAAFIYNS"
BASE COUNT          344 a          185 c          154 g          425 t
ORIGIN      
        1 tgtcagtatg ttattgaatt tattattaat atttatactt atcgttattt gtccgttaat
       61 tttcaactat actctgacgt taaatttaac atgctacatc ttagttgtca tatcgatatt
      121 tttgtcactc atctatagca tcatgaccta tactttccct tggtctatgt ttgtaacatt
      181 tataataatc agtattatca tgctattcaa acatagatat ttattttctc atacttctag
      241 tcgcttattt attaaaaaat tattatcttt ttcatataaa tttgtactct acacatcagc
      301 acttctattt ataagtacga ttcagtcacc tattgtgaaa ggtttgtcta tgtggttagc
      361 tatattatgt ctttccctgt tactaacctt tatttgttat ctcgtttgga cagtatcata
      421 tagacataga tcatacaaca aagaagttga tatcataatt gtgttaggtg ctggtatttt
      481 taccgaattt gtcacaccaa tgcttgcagc gcgtttagat cgtgcacttg atatctatca
      541 acaacaagca tcagcaacaa agatcattgt atctggcgga caaggaccag acgaaccaat
      601 ttctgaagct ttagctatgc aacgttattt gattgcacat ggtgttgata aaacagatat
      661 actaatggaa tctcactcta taaatacata cacaaacttt ttatattcta aacaaatcat
      721 caacaatttg tatcatgaag cagttaaaat tgtttgtgta acaagtcagt ttcatgtatt
      781 gcgcgcctta cgactagccc aaaaattagg tttatctatc gatggtgtcg gaagtcatac
      841 accatatcac tttttcttac atgtgctcat caaagattat ctaggcgtaa tgtatcagta
      901 tagattgtta ttaacgttat attgttcaag tagttggttt atatgtcttt acgccgcttt
      961 tatttataat agttaatcca caaacaaact attattatag gttgtttcta atattgcgtc
     1021 aaactatctt taaatattcg ttaatccttg attagacaat tgttatattg aaaataaata
     1081 aactaaagag tctgggacat taatccct
//
LOCUS       DALAGD010000216         1090 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.225, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000216 DALAGD010000000
VERSION     DALAGD010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.225"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            150..293
                     /locus_tag="O2701_002817"
     CDS             150..293
                     /locus_tag="O2701_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gallidermin/nisin family lantibiotic"
                     /protein_id="HCZ4294665.1"
                     /translation="MEKVLDLDVQVKANNNSNDSAGDERITSHSLCTPGCAKTGSFNS
                     FCC"
     gene            358..>1090
                     /locus_tag="O2701_002818"
     CDS             358..>1090
                     /locus_tag="O2701_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HCZ4294666.1"
                     /translation="MNTIYEPSSICMIRTPLLSVEFFNLFLNTEQIKYSDLQLNAQMK
                     ESILTTTFNLYCTLQEINFDGDNKKVRDAKESLLKYLIRMSTRPTPFGLLSGINLGHF
                     VNEPTRLKVGNSIQKYVKVDGEWLYKLVSYIESIDEYYQNLKVIWNSKAHIINDRIYL
                     NEQSAIYLNNNKDTSFSIKNSELLVFIKTTVTNNNITFSNLAEKINQEFEINDITKVK
                     VYIHNLVSKEIIYSTIRPPLSYSDNL"
BASE COUNT          427 a          132 c          165 g          366 t
ORIGIN      
        1 aattatcaaa atagtattac taaattaata agtgtttaca aaaaattcac aaaatataat
       61 tatataattt attgatttgt tgtgtgaaaa gggataatat gacaattgaa gtaggcctta
      121 acttcaacta ataataggag gttttatcta tggaaaaagt tcttgattta gacgtgcaag
      181 ttaaagcaaa caataactca aatgattcag caggtgacga acgtattaca agtcatagtt
      241 tatgtactcc tggttgtgct aagactggta gttttaatag cttctgctgt taagattaca
      301 gcaaatagag cctgaaaggg ctctatttgt ctattcaaat atgtgaggtg cttacaaatg
      361 aacactattt acgaaccgtc aagtatttgt atgattagaa cacctttatt atcagttgaa
      421 tttttcaatt tatttttaaa tactgaacaa attaaatata gcgacttaca attaaatgct
      481 cagatgaaag aatcaatcct aacaacaaca tttaacttat attgtacttt acaagaaata
      541 aattttgatg gcgataacaa gaaggttaga gatgctaaag aaagtttatt aaaatattta
      601 attagaatgt caacacgtcc aactccattt ggattattaa gtggtattaa tttggggcat
      661 tttgtaaatg aaccaacacg tcttaaagtt ggaaatagta ttcaaaaata tgtgaaagtc
      721 gatggcgaat ggttgtataa actggttagt tatattgaga gtattgatga atattatcaa
      781 aatttgaaag ttatttggaa tagtaaagca catattatca atgatcgaat atatttaaat
      841 gaacaaagtg ctatttattt aaacaacaat aaagatacat ctttttccat taaaaatagc
      901 gaattactcg tattcatcaa aaccactgtg acgaataata atattacttt ttcaaattta
      961 gctgaaaaga taaatcaaga atttgaaatt aatgatatta ctaaagtgaa agtatatatc
     1021 cataatcttg tctcaaagga aattatatat tcaacaatca gaccaccgtt atcatatagc
     1081 gataatttaa
//
LOCUS       DALAGD010000217         1080 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.226, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000217 DALAGD010000000
VERSION     DALAGD010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.226"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..427
                     /locus_tag="O2701_002819"
     CDS             <1..427
                     /locus_tag="O2701_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294667.1"
                     /translation="NLQFISVLTCYTVMGFVSSRYLTKYLNYKFVSEICLVIFLIIYT
                     YQSFIAVTISMIFLGISSGLTRPQTINKLSSSSNLRVMLNYAETLYFIFNIAFLLMGG
                     YLYTIGTIQYLILFISLLIFIYLIIIFYFTRREQHENKN"
     gene            411..926
                     /locus_tag="O2701_002820"
     CDS             411..926
                     /locus_tag="O2701_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HCZ4294668.1"
                     /translation="MKIKTEFKGNNIPYEYAAGADVSDSINGNPIKSFPFEVIELPEG
                     TKYLAWSLIDYDAIPVCGFAWIHWSVANVSVSGNSISIKADLSRTKGDYVQGKNSFTS
                     GLLAEDFSEIENHYVGPTPPDQDHQYELTVYALDHSLNLKNGFYLNEFLKEVNQHKID
                     QTSINLIGRKI"
BASE COUNT          392 a          123 c          168 g          397 t
ORIGIN      
        1 taatttacaa tttatttcag tattaacttg ttacacagtt atgggttttg tatcttcacg
       61 ttatttaact aaatacttga attataagtt tgtgtcagaa atttgtttag taatattttt
      121 aataatatat acatatcaaa gtttcatagc agttactatt tctatgatat ttttaggtat
      181 ttcttcaggg ttaactcgtc cacaaactat aaataaactt tctagcagta gtaacttaag
      241 agtgatgctt aattatgcag aaacgttata ttttattttt aatatcgcat ttttacttat
      301 gggtggttac ttatatacaa taggaactat tcaatactta atattattta tttcgttatt
      361 aatttttata tatttaataa taatatttta ttttacaagg agagagcaac atgaaaataa
      421 aaactgaatt taaagggaac aatataccat atgaatacgc agcaggtgca gatgtgagtg
      481 attctattaa cgggaatcca attaagtcat ttccatttga agtaattgaa ttaccggaag
      541 ggactaaata tcttgcttgg tctttaattg actatgatgc aattcctgta tgtggctttg
      601 cttggattca ttggagtgta gctaatgtaa gtgttagtgg caattcaatt tctataaaag
      661 cagatttatc aagaacaaag ggcgactatg tacaaggtaa aaatagcttt actagtgggt
      721 tgttggctga agatttttca gaaatagaaa atcactatgt aggacctaca ccacctgatc
      781 aagatcatca atatgaatta acagtttatg cgttagatca ttctttaaat ttgaagaatg
      841 ggttctactt gaatgaattt ttaaaagaag taaatcaaca taaaattgat caaacaagta
      901 ttaaccttat aggaagaaaa atttaatact aaatatctca tcaatataaa attgttcaat
      961 taaaagtaca aagaaacaaa ggttttaatt tatatattag gtacggcgtt cgctataatg
     1021 caaagaagta attaaattta agaaatgtaa acttagttat tgtaatgtga atttatttga
//
LOCUS       DALAGD010000218         1061 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.227, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000218 DALAGD010000000
VERSION     DALAGD010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.227"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            127..>1061
                     /locus_tag="O2701_002821"
     CDS             127..>1061
                     /locus_tag="O2701_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ4294669.1"
                     /translation="MAKSCLHILTNNEYATTRCQDGIVLFWPIDGEIELQKFRKSKII
                     EDDIYIINHLDVFSIKNNKKTIMLYLSSDWFAELGFTFFNYHYTAKLIKSSYNLKCLL
                     LKLTYRYLDNQPLNDADIRKLQDIIKIIAKEASMDKKIAQNQYRYAYYGDLRDELEYI
                     YQNVNQRLTLKSVADKLFVSKSNLSSQFHLLMGMGFKKYIDTLKIGKSIEILLTTDST
                     ISNISEHLGFSSSSTYSKMFKSYMDITPNEYRNLSKYNKCLMLKPEPLVGKMVQEVKE
                     IILNYIEHYKNHLTDVIHIDEDKFETPKLFQTVIQ"
BASE COUNT          409 a          128 c          165 g          359 t
ORIGIN      
        1 ccattatata gttttattta tttttgattt ggtattcgta ttgtgttaaa tttacgttta
       61 attaaataag ttaaatatta tagggaatga taaaagtggt acttaaatat agaaagaggt
      121 aaagttatgg caaaatcatg cttgcatata cttactaata atgaatatgc gacaacgcgt
      181 tgccaagatg gcatagtctt attttggcca attgacgggg aaatcgaact acaaaaattt
      241 cgtaaaagta aaataattga agatgatata tatattatta atcatctgga tgtatttagt
      301 attaagaata ataaaaaaac gatcatgttg tatttgagta gcgattggtt tgcggaatta
      361 ggctttactt tctttaatta ccactataca gcaaagttga ttaaatcatc ctataatttg
      421 aaatgtctac tattaaaatt gacatatcga taccttgata atcagcctct taatgacgct
      481 gatattagaa aattacagga tattattaaa atcattgcaa aagaagcaag tatggataaa
      541 aagattgcac aaaatcaata tcgatatgcg tattatggtg atttgcgtga tgagctcgaa
      601 tatatttatc aaaatgtaaa tcaacgattg acattaaaaa gtgtcgctga taaattattt
      661 gtctcaaagt caaatttgtc atcacaattc cacttactta tgggcatggg ttttaaaaaa
      721 tatattgata ctttgaaaat tggtaaatcg attgaaattc tacttactac tgatagtact
      781 attagcaaca taagtgaaca tttaggtttt agtagtagct ccacttactc taaaatgttt
      841 aaaagttata tggatataac accgaatgaa tatcgtaatt tatcaaaata taataaatgt
      901 ttaatgctaa agccagaacc actagtaggc aaaatggtgc aagaagtaaa agaaatcata
      961 ttgaattata ttgaacatta taaaaaccac ctaactgatg ttatacatat tgatgaagac
     1021 aaatttgaaa cacctaaatt gtttcaaacg gttattcaaa t
//
LOCUS       DALAGD010000219         1055 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.228, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000219 DALAGD010000000
VERSION     DALAGD010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.228"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            109..729
                     /locus_tag="O2701_002822"
     CDS             109..729
                     /locus_tag="O2701_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294670.1"
                     /translation="MKFTEVEVIEHLVKAYKEAGKPTYPHENLYRGRNHSISGIGEDL
                     LGAYLISRLEGVQIFIDQPLSMIDKSLSTRYPDLLICEDNEIKNILEVKMDLGYQRKD
                     FIDYCRKKEEWISNIVGKQCVLSRKREDKIPMNIADDIKFHVVIYSENNGPKRFDEEI
                     MPIVNETCPHIEVYVLTSGQHPNLVNVNLEGININKDEFEILVNAL"
BASE COUNT          366 a          130 c          187 g          372 t
ORIGIN      
        1 cattcaaccc tcttaattta ttaggaggtt ttttgtattt atgctttcaa atgtgtgata
       61 tactttgttt gttgaaatat agagtatcta tagatagggt gattgagtat gaaattcact
      121 gaagtggaag ttatcgaaca tcttgtaaag gcatataaag aagcaggaaa gcctacttat
      181 cctcatgaaa atttatatcg aggacgtaat catagtattt caggtattgg agaagacttg
      241 ctgggtgctt atttgattag tagattggaa ggtgtccaaa tatttattga tcagccttta
      301 tctatgattg ataaatcttt aagtacaaga tatccggatt tattaatttg tgaagataat
      361 gaaattaaaa atatactaga agttaaaatg gacttaggat atcaaagaaa agattttata
      421 gattattgcc gaaagaaaga agaatggatt tcaaatatcg taggaaaaca gtgtgtattg
      481 tctagaaaga gagaagacaa aattcctatg aatatagctg atgatattaa atttcatgtt
      541 gtgatttaca gtgaaaacaa tggaccgaag cggtttgatg aagaaatcat gcctatcgtt
      601 aatgaaacat gtccacatat tgaagtatat gtcctaacaa gcggtcaaca ccctaattta
      661 gtaaatgtta atcttgaagg tattaatatt aataaagatg aatttgaaat attagtaaat
      721 gcgttataaa aaaatagagc atcctccacg ttatggaggt gctctgtttt ttattgaaaa
      781 gtatcaagtt aattaattta atatgcttaa taagttctac cttgaccttt ttctctagct
      841 tctgttcgat ctcttatgta ctcagtacat actggatttt ctgttaattc atcaactgta
      901 ttagtttaat cagaagacgt gtctactttg taagcttcta agaacttatt atatgatata
      961 gcgtttgagt tttgttgttc ttctataaat ttctgtagtt atttttcaaa aaccgcatca
     1021 ttaactgata agcagaagcg tatcacaaat aaaac
//
LOCUS       DALAGD010000220          992 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.229, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000220 DALAGD010000000
VERSION     DALAGD010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.229"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(238..669)
                     /locus_tag="O2701_002823"
     CDS             complement(238..669)
                     /locus_tag="O2701_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCZ4294671.1"
                     /translation="MFKNILLPYDFENDFSAIPDYLEKVTDEDSVVVIYHVVTENDLA
                     ISVKYYNKHKEDIIREKEKKLTPFLRELEKRDIQYKIDVDFGHIKDTILEKITSGDIN
                     NGEFDLVIMSNHRVDLNIKHVLGDVTHKIAKRSSVPVLIVK"
BASE COUNT          320 a          155 c           96 g          421 t
ORIGIN      
        1 attcaacttt gcaacagaac ctatattttg ctataattat taaattaatt gcctttgtgt
       61 tagtattccc tggattacta acactttggt tagctgttct aagtgataca ggtgcagcaa
      121 tattagttat attaaattca ttacgtttat taaaaaaata aaaaagccta aatactctct
      181 cgatttgtga gaaagtattt aggctttttg aattaataaa tttcttactt cttatgttta
      241 tttaacaatt agtactggga cagaacttct tttagcaatc ttatgtgtaa catctcctaa
      301 aacatgttta atattcaaat cgactctatg attactcata attactaaat caaattcacc
      361 attatttata tctccagaag taattttttc taagattgta tctttaatat gcccaaaatc
      421 tacatctatt ttatattgaa tatctctttt ttctaattca cgtaaaaatg gagtgagttt
      481 tttctctttt tctctaataa tatcttcttt atgcttatta taatacttga cactaattgc
      541 aagatcattt tctgttacaa cgtgataaat tacaacaact gaatcttcat cggtgacttt
      601 ttctaaatag tcagggatag cactaaaatc attttcgaaa tcatagggta ataatatatt
      661 tttgaacatc tacaccagcc tcctattact tattatttag atgtctatta cttatatata
      721 ccctaaatat gtatgcttat tctcatattc aaattattca aatatattcc ttatcatgta
      781 ttagattcta ttttttatat tatttttgta attctttgta taaacctatt tatttataaa
      841 gcgctctaat acatacctaa atatcttcaa cttataaact actttttatt tcaatgcctt
      901 tttccaaact tcttttatga gatattgtgg ttattacctg acatttacaa tttacacatt
      961 tcgctattat tacgcaaatt attttgattt ta
//
LOCUS       DALAGD010000221          977 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.230, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000221 DALAGD010000000
VERSION     DALAGD010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.230"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(201..305)
                     /locus_tag="O2701_002824"
     CDS             complement(201..305)
                     /locus_tag="O2701_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294672.1"
                     /translation="MLNIKAIKPSEKGIKVLSHQFKQVSKTQLNYFFN"
     gene            complement(468..602)
                     /locus_tag="O2701_002825"
     CDS             complement(468..602)
                     /locus_tag="O2701_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294673.1"
                     /translation="MTDKNGHGIAITGIILFVINALQINNDLPISISSKLKKYIHKPD
                     "
     gene            complement(696..953)
                     /locus_tag="O2701_002826"
     CDS             complement(696..953)
                     /locus_tag="O2701_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294674.1"
                     /translation="MEIIIEIYMYLRDGIKEHFILKKVRKKFKKLNEINSDIQNMYAE
                     NQGLLETDKTIIDKVLSTDVTNSHDINELYLFLKNYLDKTQ"
BASE COUNT          326 a          151 c           92 g          408 t
ORIGIN      
        1 tgaaattata ttattacttc tacaaatttc aaattgccgt aattgaacgt atatttcttc
       61 ttcaactatt atttcatctt tagcataatc tatatataaa attttatgct attatttaaa
      121 taattcgcta taacttaaca tacgttttcg atataaacct tgttctaaat ctcaataatt
      181 ttttgctgtt ttcatcgtca ttagttaaaa aaataattta actgagtttt tgagacctgt
      241 ttaaattgat gactcaaaac ctttatcccc ttttcactcg gtttaattgc tttaatattt
      301 aacacagtct cattgtaatt ttgctactaa gtttgaaata tttcgattcg aatgtgaaac
      361 atcatttatt attactttcg acagctagaa aaatattgtt aaaccaaaac tataattacc
      421 acttttatat agaactatat ataaatttta actttgagac agaactgcta gtcaggttta
      481 tgaatatatt tctttagttt acttgatata cttattggta aatcattatt tatttgaaga
      541 gcatttataa caaaaagtat aatcccagtt atagcgattc catgaccatt tttatcagtc
      601 aaagattcat caacaaaaac tttttgataa atttatatat ttgtataatt tattatggta
      661 gatatctaca cccgtatcta taacagctac agttatcatt gcgtcttatc caaatagttt
      721 ttaagaaata aatataattc attaatatca tggctatttg taacgtcagt gcttaagacc
      781 ttgtcaatta ttgttttatc tgtttctaag agcccctgat tttctgcata catattttgt
      841 atatcgctgt taatctcatt taatttttta aatttttttc taaccttttt caaaataaaa
      901 tgctctttaa ttccatctct taaatacata taaatctcta ttataatctc cataaatgtc
      961 atatctcctc tctcaaa
//
LOCUS       DALAGD010000222          953 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.231, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000222 DALAGD010000000
VERSION     DALAGD010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.231"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(119..>953)
                     /locus_tag="O2701_002827"
     CDS             complement(119..>953)
                     /locus_tag="O2701_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HCZ4294675.1"
                     /translation="LELDVNGNFLVPDKYKQGTHFKEVFSLYKLDRKFRNVLLEYLLV
                     FETHIKSRISYYFSEKYREPHSYLYFKNYSSDTSKTDSIVKMVATFSSVMSNRKNKPL
                     KHYINTHNGVPLWILVNYLTLGNVSKMYSNLDDDLRLEVAKDYKRKLERDYKTRVQIT
                     PSDVDSILQQAHMFRNVCAHEERLYDYKIDRAKSRANIFANYNKIYDKEYVPTMNGSY
                     VFDLLISLCLFLNKHDYIKLMKNMDKLISNYSHSFYTITIDDLYTKMNFPDQTKILDM
                     L"
BASE COUNT          323 a          155 c          108 g          367 t
ORIGIN      
        1 ctatatctat actttatcta tttctatata cacactcatc cctcactgca acacagggcg
       61 tttctcagcg tcttaaaata aaaaaacgcc actcgtaagt gacattaaaa aatatctttt
      121 ataacatatc cagtattttt gtttgatctg gaaaattcat ttttgtatat aggtcatcta
      181 tagtaattgt atagaaagaa tgtgaataat tacttattag tttatccata tttttcatca
      241 attttatgta atcatgttta ttcaaaaata gacatagtga aatcaacaaa tcgaatacat
      301 aactaccatt cattgtagga acgtattctt tatcgtatat tttgttataa ttggcgaata
      361 tattagctct acttttagcc ctgtctattt tataatcata caatctttct tcatgcgcac
      421 acacgttacg aaacatatgt gcttgttgta gtatactgtc tacatctgat ggagttattt
      481 gaacacgtgt tttataatct ctttccaatt tccttttata gtctttagca acttccaatc
      541 gaagatcatc atccaaattg gaatacattt ttgaaacatt acctaaagtt aaataattca
      601 ccaatatcca tagtggcact ccattatgag tattaatata atgttttaat ggtttatttt
      661 ttctattact cataaccgag ctaaatgtag caaccatttt cacgatgcta tctgtcttac
      721 ttgtgtcaga tgaataattt ttaaagtata aatatgaatg tggttctcta tatttttcgc
      781 taaaataata tgaaattctt gatttaatat gagtttcaaa tactaacaaa tactctaata
      841 aaacattcct aaattttcta tctagtttgt ataaagaaaa gacttcttta aaatgagtac
      901 cttgcttata tttatcagga accaaaaaat taccattaac atctagttct aaa
//
LOCUS       DALAGD010000223          877 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.232, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000223 DALAGD010000000
VERSION     DALAGD010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.232"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            113..652
                     /locus_tag="O2701_002828"
     CDS             113..652
                     /locus_tag="O2701_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294676.1"
                     /translation="MVAYASHLSFLLFSNKKVLKINAYTGTDMAKLDLNSLDDEHVKL
                     LINELKYPETHIDVNELKTIVASRINERQEIISFKLGIKYLLTIKRGNIEKDRFSISI
                     IFKDTYHTLVRIDINGGTHDNPDGTIAPKSHIHIYNDKYDKKDRFAYEINLKDFPDIY
                     NLYNVYMSFLEYNNIKDLE"
     gene            665..>877
                     /locus_tag="O2701_002829"
     CDS             665..>877
                     /locus_tag="O2701_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1828 domain-containing protein"
                     /protein_id="HCZ4294677.1"
                     /translation="MTTFDAKKLKKEYLDWYNQTLEFSNLSNNVVRIDTPFKDNSLDN
                     LIIYALYDQSRDMITLTDDGYTIFDLE"
BASE COUNT          351 a          110 c          129 g          287 t
ORIGIN      
        1 aatacttaat tttaaaaatc ccctatgaaa atgttgatga gctacatctt taaatcttat
       61 tatacattgt ataaaattat attgcgaggt agtaaattga tattatacac ttttggttgc
      121 gtatgcatct cacctatctt ttttgctttt ttcaaataag aaagtattaa aaattaatgc
      181 ctatacagga acagacatgg ctaaacttga tttaaatagt cttgacgacg agcacgtaaa
      241 attattaata aatgaattaa aatatccaga aactcatatc gatgtaaatg aattaaaaac
      301 aatagttgct agtcgaataa atgaaaggca agaaataata agttttaagt taggaataaa
      361 gtacttatta acaataaaaa gagggaacat agaaaaagat aggttttcaa tttcaatcat
      421 tttcaaagat acctatcaca ccctagttag aatagatatt aacggtggta ctcacgataa
      481 tccagatgga acaatcgctc cgaaaagtca tattcacata tataatgata agtatgataa
      541 aaaggatagg tttgcttatg aaattaactt gaaagatttc cccgacatct ataacttgta
      601 taatgtgtat atgtcgtttt tagagtataa taatataaaa gaccttgaat aaaggaggga
      661 taatatgact acatttgatg ctaaaaaatt aaaaaaagaa tatcttgatt ggtataatca
      721 gaccttagag ttttctaatt tatcaaacaa tgtagtaaga atagatactc cttttaaaga
      781 taattcttta gataatttaa taatttacgc tttatacgat cagtccagag acatgattac
      841 actgacagat gacggctata ctatatttga tttagaa
//
LOCUS       DALAGD010000224          858 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.233, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000224 DALAGD010000000
VERSION     DALAGD010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.233"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(258..>858)
                     /gene="icaC"
                     /locus_tag="O2701_002830"
     CDS             complement(258..>858)
                     /gene="icaC"
                     /locus_tag="O2701_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide intercellular adhesin
                     biosynthesis/export protein IcaC"
                     /protein_id="HCZ4294678.1"
                     /translation="LLLSFILQQSFLYYFTNNTAFHDTVLHYYPLSENTIIFGWIFYF
                     FLGAYMGYNYERVLNFLERYLVIMIVLAVATYFVFIALANGDYWNVTSFSYSLTPYNS
                     IMFIVILGICTHFKTMLFNTIQMISAFSFFIYLLHPIILDSLFAYTNIFEDNTMVFLA
                     ISLLFILGLCIGVGMILREFYIFRFIIGKQPYKLNINAY"
BASE COUNT          339 a          131 c          136 g          252 t
ORIGIN      
        1 gcttacaata atgtgcaagt tggcggggcc ccaacataga agctggcgga aagtcaggtt
       61 acaataatgt gcaagttggg gtgggacgac gaaataaatt ttgcgaaaat atcatttctg
      121 tcccactccc attggcattt acgaagttta aatgtgcaat tagaatatat gtataacaat
      181 attaaacacg cggtaaaacg aagtcagtca attcaaactg atttcgccca ccgcgtgttt
      241 ttaacatagc ttaataatta ataagcatta atgttcaatt tatatggttg ttttccaata
      301 ataaacctaa agatatagaa ttcacgcaat atcatgccga cacctataca taatcctaaa
      361 atgaatagta gtgatatcgc tagaaagacc attgtattat cctcaaatat atttgtatat
      421 gcaaacaatg agtctagaat gattggatgt aataaataaa taaagaatga gaaagcacta
      481 atcatttgaa tcgtattaaa taacattgtt ttaaaatgcg tgcaaatacc caagataaca
      541 ataaacataa tactattata tggtgttaat gaatatgaaa agctggtaac gttccaatag
      601 tctccatttg ctaacgcaat aaacacaaaa taagtagcta cagctaatac aatcataata
      661 actaaataac gttctaagaa atttaataca cgttcgtagt tataacccat atatgcacct
      721 aagaagaaat aaaaaatcca tccgaatatt atagtatttt cacttaatgg ataatagtgt
      781 agcacggtat cgtgaaacgc tgtgttgttc gtaaagtaat ataaaaatga ttgctgtaaa
      841 ataaaagata acaataat
//
LOCUS       DALAGD010000225          830 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.234, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000225 DALAGD010000000
VERSION     DALAGD010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.234"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(<1..293)
                     /locus_tag="O2701_002831"
     CDS             complement(<1..293)
                     /locus_tag="O2701_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002434158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HCZ4294679.1"
                     /translation="MENTNSNMHKNDNSYVLSALSYLSIFFAPVILPLFVWILADEPT
                     SEHGKKAFINHIMTWVSFFIGRLAFIFSKEVFDKPLDHQLLIFSILLIITIIF"
BASE COUNT          328 a          106 c          112 g          284 t
ORIGIN      
        1 aagaatataa tagtaataat taaaagaatg ctaaatatta ataattgatg atccaaaggt
       61 ttatcaaaga cttctttaga aaaaataaat gccaatctac ctataaaaaa actaacccaa
      121 gtcataatat gattaatgaa tgcttttttg ccatgttctg atgttggttc atcagcaagt
      181 atccatacaa ataaaggtaa aatgacaggt gcgaaaaaga tacttaaata actcaatgct
      241 gataaaacgt aactattgtc atttttatgc atattactat ttgtattttc cacttttttc
      301 accttcttta cttttcttta ctattataaa aacattaata gcgagctatt acaacagtta
      361 taagagaagc ttacgatatt gtaagtatgt gttgttataa tcaatataaa attaagaaag
      421 acctgagaat ttatattatc atccacaatc gaagtgagta gcgaaattta ttggaaaagg
      481 tacttatatt gaagatattg ttaatggttc atttttaaaa agccaagacg atttgaagtt
      541 aaaagcttct gacttcactg tcgaagataa ctataactac ggtgtactta tatttccaga
      601 gaatctttct cttacagata ataataagaa attggaatct ttaataaaaa ctatactatt
      661 tacaggtaaa cgatatcaat atacttattt taaaatttga tcttaataga taaggatata
      721 atgatttaaa caatttaaga gatattattt tcacaaaaaa agaatgcttt ttgttagagg
      781 cactgaaccc ttaaaatggg aacttaataa aaacactatg ttctaggctg
//
LOCUS       DALAGD010000226          822 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.235, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000226 DALAGD010000000
VERSION     DALAGD010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.235"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..404
                     /locus_tag="O2701_002832"
     CDS             <1..404
                     /locus_tag="O2701_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCZ4294680.1"
                     /translation="KKYPVKMINNKFISTEEVKEENIKKEIENFKFFAQYSNFKDLMN
                     YKDGDISYNPEVPSYSAQYQLTNDDYNVKQLRKRYDIPTSKAPKLLLKGSGNLKGSSV
                     GYKDIEFTFVEKKEENIYFSDSLDYKKSGDV"
     gene            456..>822
                     /locus_tag="O2701_002833"
     CDS             456..>822
                     /locus_tag="O2701_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCZ4294681.1"
                     /translation="MIKRVNKLVLGISLLFLVISITAGCGMGKEAEIKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDQFDKNDKGTWIVNSQMAIQNKGEALKIKGMLLKIDRNTRSA
                     KGFYYTNEIKTEKYEVAQDN"
BASE COUNT          357 a           81 c          154 g          230 t
ORIGIN      
        1 aaaaaaaata tccagttaaa atgattaata ataaattcat ttctactgag gaagttaaag
       61 aagaaaacat aaaaaaagaa atcgaaaact ttaagttttt tgcgcaatat agcaatttta
      121 aagatttaat gaattataaa gatggagata tatcatataa tccagaggtg ccgagttatt
      181 cagctcaata tcaattaact aatgatgatt ataatgtaaa acaattacgc aaaagatatg
      241 atataccgac gagtaaagct ccaaagttat tgttaaaagg ttcagggaat ttaaaaggct
      301 catcagttgg atataaagat attgaattta cgtttgtaga gaaaaaagaa gaaaatatat
      361 actttagtga tagcttagat tataaaaaaa gcggagatgt ataatcatgg ctcaatcaga
      421 atatgaaatc aatcccggaa aaagagagtg atgaaatgat aaaacgtgta aataaattag
      481 tgcttggtat tagtcttctg tttttagtca ttagtatcac tgctggttgt ggcatgggta
      541 aagaagcgga aataaagaaa agttttgaaa aaacattgag tatgtatccg attaaaaatc
      601 tagaggattt atacgataaa gaaggatatc gtgatgatca atttgataaa aatgataagg
      661 gaacatggat tgtaaattct caaatggcaa ttcaaaataa aggagaagct ctaaaaataa
      721 aaggcatgct tttgaagata gatagaaata caagaagtgc aaaaggattt tactatacta
      781 atgaaataaa gacggagaaa tacgaagtag ctcaggataa tc
//
LOCUS       DALAGD010000227          805 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.236, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000227 DALAGD010000000
VERSION     DALAGD010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.236"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            103..451
                     /locus_tag="O2701_002834"
                     /pseudo
     CDS             103..451
                     /locus_tag="O2701_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456552.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            462..656
                     /locus_tag="O2701_002835"
     CDS             462..656
                     /locus_tag="O2701_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294682.1"
                     /translation="MRIEDAYRKDVIITLLNNEEYEGFVTDYENEFESETGNLVVDIQ
                     TDFAVYSFDETEIKSIRLLK"
BASE COUNT          358 a           73 c          144 g          230 t
ORIGIN      
        1 ggcaattggc gggacaagtt ctttaaagag cgtgaaggta aatatcaagt agaagtaaaa
       61 gaagcataat tacaggaaaa agctaaaaac cagatgaaag aaatgattaa aaatggtaaa
      121 ataaaaatag aaataaattg cggaaaaata aaatagatat atgttaggtc atcacctata
      181 taatgaaaat aaaaaaagag ccattttaaa taataagaaa ttgcctagct atacaatact
      241 ttctatagat ttattgaatg aattgttaag agaaaaaatg tcaacaggca atctaatatt
      301 aagtgatgag cgatttgatt tgaaagagat tattaatttt aatcaaatta ttggaaaaat
      361 acatatcgaa aatgtgtata ttgaaaccag aaagggaaaa gtgcattatt cgaagacagg
      421 tgctcatata gtaccttata ttgataagta ggtgaaaagt attgagaatt gaagatgcat
      481 atcgtaaaga tgttattatc acgcttttga ataatgaaga atacgaaggg tttgtaactg
      541 actatgaaaa tgaattcgag agcgaaacag gaaatcttgt tgtagatata cagactgatt
      601 ttgctgttta ttcgtttgat gaaactgaga taaaaagtat tagattatta aaataattac
      661 aaaatagcat cctttctaca aaaagctaaa aaagaaatgc ttgaaatgat aagtaatggt
      721 aaaatgacga tgaaattgaa taatgttaag caaaagcgtc atattttatg cacaaatgaa
      781 tataataata agaaaaataa ttcat
//
LOCUS       DALAGD010000228          793 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.237, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000228 DALAGD010000000
VERSION     DALAGD010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.237"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            187..393
                     /locus_tag="O2701_002836"
     CDS             187..393
                     /locus_tag="O2701_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294683.1"
                     /translation="MNEIETIISEIEKLLTNNTPYSISKNSGVPRQTVTDLKVGKTKI
                     KEAKFKTIIKLYEYQRTLENKTEC"
BASE COUNT          332 a           76 c          112 g          273 t
ORIGIN      
        1 aaataatata atatagaata gatggagaca agaaatttta tcaatatata aaaaaaacgc
       61 caatactatt aggtattagt ttatgaatta actagtttgg caactagaaa aaaacaatgt
      121 attgctaaac gtagttactg cattaggatt attataatta taaatagaaa gagagtgtga
      181 ttaataatga atgagattga aactattata agtgaaatag aaaagttatt aactaacaat
      241 acaccatata gtatttcaaa aaactcaggt gtaccacgtc aaacagttac tgatttaaag
      301 gtaggtaaaa ctaaaataaa agaagctaaa tttaaaacga taatcaagtt atatgaatat
      361 caaagaacat tagaaaataa aacagaatgt taacaaaaat attttgttag gttaggtgga
      421 caagaaatta aatttctaat gatttcatgg ttgtctgtcc acgttttgtc cacctcaata
      481 agataatctc ttagttttag aaattgtaca tggttaaata atcaaaaatg catattatga
      541 aattatcatt gccattaaat cttgaatgat ctttaagcga tgatataaaa ggctgaacac
      601 agtaaataag ttaactttaa tgcagtattc tatactatac atattttgta ataatgttta
      661 tgcacataat ttttcattga caatttttta tattaaattg tgtaattggc atatatgatg
      721 atttgtgata attatattgt aatatagcat ttggttttat gattctaaaa ttaatagatt
      781 tgaaaagtat act
//
LOCUS       DALAGD010000229          789 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.238, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000229 DALAGD010000000
VERSION     DALAGD010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.238"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..>789
                     /locus_tag="O2701_002837"
     CDS             <1..>789
                     /locus_tag="O2701_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCZ4294684.1"
                     /translation="ERLYASVLGATLRIDICEIHIEIANYIYEEIFDKEMVYPHILMR
                     DYARQTIEYISLSKDISNINLEKIRPPYKSNWYKKEYSNLNIDDYIKSLKNKLDSHLH
                     FSIDKIKNSMTTEYGRGTGAYGDFGRYVFGYAVRNWVKGFKSDQDLSNIALMRIFEMG
                     YDAKLHGEFDMWVNRYDNFNNSIERISKKYQWIAYYEILAKLVDKFPDVQYSGLWDDY
                     IRDIDPTLLLLEIDKESKILVPSPLPSHQSNEWVKNTKVFDETKL"
BASE COUNT          310 a           85 c          132 g          262 t
ORIGIN      
        1 gaaagattat atgcttctgt tttaggggcg acacttcgaa tcgacatttg tgaaatacat
       61 atagagattg ctaattatat ttatgaagaa atatttgata aagaaatggt atatcctcat
      121 attttaatga gagattatgc taggcaaaca attgaatata ttagtttatc aaaagatata
      181 tctaatataa atttagaaaa aattaggcca ccttataaaa gtaattggta taaaaaagaa
      241 tattcgaatt taaatataga cgattacata aaatctttaa aaaacaaatt agatagccat
      301 ttacattttt ctatcgataa aattaaaaac tcaatgacaa cggaatatgg aagaggaact
      361 ggggcatatg gcgattttgg cagatatgta tttggttatg ctgtacgaaa ctgggtaaag
      421 ggttttaaaa gtgatcaaga cttaagtaat atagcattaa tgaggatttt tgaaatgggt
      481 tatgatgcaa aattacatgg tgaatttgat atgtgggtta atcgttatga taactttaat
      541 aattcaattg aaagaattag caaaaaatat caatggattg cttactatga aattttagct
      601 aaacttgtag ataaatttcc tgatgttcaa tattctggac tatgggatga ttatattaga
      661 gatattgatc ctactttact attacttgaa attgataaag aatcaaaaat actagtaccc
      721 tcaccacttc cttcgcatca atcgaatgaa tgggtaaaaa ataccaaagt ttttgatgaa
      781 acaaaatta
//
LOCUS       DALAGD010000230          701 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.239, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000230 DALAGD010000000
VERSION     DALAGD010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.239"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
BASE COUNT          290 a           96 c           82 g          233 t
ORIGIN      
        1 aaatagaata attttttaac atagttttgt tgttttgaat tataaaaact aaagtaaaaa
       61 tgttggatgt gaaagattgt tgatgagaat tttgagatgt agataaatat caaactagtc
      121 tataggtaaa aatttataat taatttgaaa agaatgtctc aaataatgtt tgaattagca
      181 ggtgtcatac taagtaatgt taagtaaaca ttttatactt tatcagaaca aaaaagacat
      241 gcatcaacat gtcagtgaaa ctaatccata ttatcttaac aacgaaattg aatcgaacct
      301 gaaaaacgta tttacaaatc gcaactactc tatccaaggc aaaatcgcaa aatctgatcc
      361 ccgaaataac atatctcgaa aaaaattcca taattcaatc atcttcaata tttaacaata
      421 cattactagt tccaaatata ctcatacata catgtttcat tgattgactg catattgtca
      481 taaataatag gggaactaat cgctaataat gttgttaaac acaatacata gtaaagtgaa
      541 agattataaa tacatgaatt gttcaccatt ccgccatata ttttcaatca cctaatttcc
      601 gaataaatat tacaatttta attcaataat taatatttaa gaccaaaata atcctaaaaa
      661 gtcaatttta attgcgtgat attgagtttt aagcatattt a
//
LOCUS       DALAGD010000231          681 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.240, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000231 DALAGD010000000
VERSION     DALAGD010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.240"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..>681
                     /gene="ccsA"
                     /locus_tag="O2701_002838"
     CDS             <1..>681
                     /gene="ccsA"
                     /locus_tag="O2701_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HCZ4294685.1"
                     /translation="ICYFYDFVQKSHKIRSLGIYLLGIVWVLQTISLSIFIIQTRHIP
                     LGSISDVFYTLSWLIISISLILNLIKVLNFSVFFLNLIGLTLLGMNTFQPTHYTNKVQ
                     KIAVVDELLLVHIGLAVLSYVFFALAFVNALLYIMQYRNLKEKRFDQKYFRIGSVATL
                     ESIVFYSTLSGWIILIFSIILGTQWGVISVGERIFIDPKVILSSIITVLYGSYILLRI
                     NKWLNSRY"
BASE COUNT          202 a           90 c          113 g          276 t
ORIGIN      
        1 catttgctat ttttatgatt ttgtacaaaa aagtcataag attagaagtt taggcatata
       61 tttattgggg attgtttggg ttttacaaac aatctcttta tctattttta ttatacaaac
      121 tagacatatt ccattagggt ctatttcaga tgtattctat actttaagtt ggctgattat
      181 ttcaatctct ttaattctta accttatcaa agttttgaat ttctctgtgt ttttcttgaa
      241 tttaataggg ttaacacttt tagggatgaa tacatttcaa ccgacacatt atactaataa
      301 ggttcagaaa attgctgttg tagacgagtt actgttagtc catatcggtc tagctgtgtt
      361 aagttatgtc ttctttgcct tagcttttgt caatgcatta ctgtatatca tgcaataccg
      421 taatttgaag gaaaaacgtt ttgatcaaaa atactttaga attggtagcg ttgctacact
      481 tgaatcaatt gtgttttact caacgttaag tgggtggata attctcatat ttagtattat
      541 tttaggtaca cagtggggtg taatttcagt tggtgaacgt atattcattg acccgaaggt
      601 aatactctct tcaattatta cagtattata tggaagttat attttactta gaatcaacaa
      661 atggttgaat tcaagatatt t
//
LOCUS       DALAGD010000232          676 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.241, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000232 DALAGD010000000
VERSION     DALAGD010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.241"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..187
                     /locus_tag="O2701_002839"
     CDS             <1..187
                     /locus_tag="O2701_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294686.1"
                     /translation="NDENIKPGIIQMDIDSLEVINAEKSSLEKNTRDNYFIHAIDRIY
                     INTSKGLFPESELVAWG"
     gene            222..344
                     /locus_tag="O2701_002840"
     CDS             222..344
                     /locus_tag="O2701_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294687.1"
                     /translation="MKVIFYYPKRADDILKASMEYQSTDYKRRYISWKTMIAMK"
     gene            320..418
                     /locus_tag="O2701_002841"
                     /pseudo
     CDS             320..418
                     /locus_tag="O2701_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429703.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="head morphogenesis protein"
BASE COUNT          264 a           84 c          108 g          220 t
ORIGIN      
        1 taatgatgaa aatattaagc ctggtattat tcaaatggat atagatagtc ttgaagttat
       61 caatgctgaa aaatctagtt tagaaaaaaa tacaagagat aattatttta ttcatgccat
      121 agacagaata tacataaata ctagtaaagg gctatttcct gaatctgaac tcgtagcatg
      181 gggataaaga aagtttactt taaaaatttt aaccttcaaa tgtgaaggtt attttttatt
      241 acccaaaacg tgctgatgac attttaaaag caagtatgga atatcagtcg acagactata
      301 aacggaggta tatctcatgg aaaacaatga tagcaatgaa gtagcttatt tgttaaaaga
      361 tggtaaattt acaaaagtat atgtcaatca agatagtgta tcttttgtgc cagggtaaca
      421 agcaaccgaa ttattattta acagtaaacc gaattcaatt gttatgttac ataaccatcc
      481 tggacagtca ggattttcag aatatgattt atttacattt tttaagcatc catcaataaa
      541 atcaatgaca atcgttacta ataagggaca agtaaaattt attaccaagt caaataggtt
      601 tcatggtaaa atagtgagta aattttgtgc taaatatttt acgcatatta atatcattaa
      661 tgatagccat attgaa
//
LOCUS       DALAGD010000233          671 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.242, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000233 DALAGD010000000
VERSION     DALAGD010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.242"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(77..631)
                     /locus_tag="O2701_002842"
     CDS             complement(77..631)
                     /locus_tag="O2701_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4888 domain-containing protein"
                     /protein_id="HCZ4294688.1"
                     /translation="MNKKLLTKTLIASALVLTTVGSGFHSSSNYNGINNVAKAEQTTD
                     NELWKNVRDALKEANIIDKTDNENVKVTYKLANGGENSIEGTANLNELSNSNNKPINS
                     DSVKSIDLSRKNPNGDRFDANDAWKKLTDKLKEKQIVKDGDTVTIHSKDEKDPKISAK
                     VGEVYNDNKRLMLFKKDIDKITIN"
BASE COUNT          189 a          116 c           79 g          287 t
ORIGIN      
        1 ataaattaat atttgagata ctgcctctta aaaaaagaca agttacatta aaaaagtaac
       61 ctgtctttat ctataactag tttatagtga ttttatctat atccttttta aacaacatca
      121 atcgtttgtt atcattatag acttcaccga ctttagctga gattttaggg tctttttcgt
      181 ctttactatg aatagttact gtatcaccgt ctttcacaat ctgcttttct tttaatttat
      241 cagttaattt tttccatgca tcatttgcgt caaatctatc tccattggga tttttccttg
      301 acaaatcaat gctttttaca ctatcgctat tgatcggttt gttattagaa ttgctaagtt
      361 catttaaatt ggcggtacct tcaatactgt tttcaccgcc atttgctaat ttataagtca
      421 ctttgacatt ttcattatct gttttatcga taatattcgc ttcttttaaa gcgtctctta
      481 catttttcca caattcgtta tctgtcgttt gctcagcttt tgcaacgtta ttaataccat
      541 tataatttga agaagaatga aaacctgaac ctactgttgt taaaactaaa gcacttgcta
      601 tcaatgtttt tgttaatagt tttttattca ttttattttc tcctataatt tatttgcaat
      661 cgattacaaa g
//
LOCUS       DALAGD010000234          661 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.243, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000234 DALAGD010000000
VERSION     DALAGD010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.243"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..538
                     /locus_tag="O2701_002843"
     CDS             <1..538
                     /locus_tag="O2701_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCZ4294689.1"
                     /translation="TQNKGEALKVKGMVLYMNRNTKTTKGYYYVNAIKNDKDGRPQEN
                     EKRYPVKMVDNKIIPTKEIKDKNIKKEIENFKFFVQYGNFKDLSKYKDGDISYNPEVP
                     SYSAKYQLTNDDYNVKQLRKRYDIPTNKAPKLLLKGTGNLKGSSVGYKDIEFTFVEKK
                     EENIYFSDGLIFKPSEDK"
BASE COUNT          291 a           69 c          121 g          180 t
ORIGIN      
        1 aactcaaaat aagggggaag ctctgaaagt taaaggtatg gtcttgtata tgaatagaaa
       61 taccaaaaca acaaaaggat attattatgt aaatgcaata aagaatgata aagacggaag
      121 accccaagag aatgaaaaaa ggtacccagt taaaatggtc gataataaaa tcattccaac
      181 aaaagaaatt aaagataaaa acataaaaaa agaaatcgaa aactttaagt tctttgttca
      241 atatggaaac tttaaagatt tgtcgaagta taaagacgga gatatttcat ataatccaga
      301 ggtgccgagt tattcggcta aatatcaatt aactaatgat gattacaatg taaagcaatt
      361 acgtaaaaga tatgacatac caacaaataa agcaccgaag ttgttgttga agggtacagg
      421 gaatttaaaa ggttcatcag ttggatataa agacattgaa tttacgtttg tagagaaaaa
      481 agaagagaac atttacttta gtgatgggtt aatttttaaa ccaagtgagg ataaataatc
      541 atgactcgat caaaatatga aatcaatcca ggaaagataa taagaagaat aggattgtga
      601 aattatgaaa cgattaaaca aattggtatt gtatattagt tttttgatat tagtcattag
      661 t
//
LOCUS       DALAGD010000235          644 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.244, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000235 DALAGD010000000
VERSION     DALAGD010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.244"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            245..495
                     /locus_tag="O2701_002844"
                     /pseudo
     CDS             245..495
                     /locus_tag="O2701_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187510.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
BASE COUNT          253 a           86 c           77 g          228 t
ORIGIN      
        1 ttgattaact aattaattta caatacctaa aatgttgttt ggttttgttt ataccaagct
       61 tcaaacttaa atgtcataac aacattcatt tcttaattcc gattagattt gtcgattata
      121 tttacagcat ctttatactc aaaaaacatt tacttaaaaa tataaattcg atttaataat
      181 taatttaaat ttagttaatc aattttgcat ctattttgtt gtaagctata taaaaggagt
      241 gataatgatg gtgaaaaaaa caaaatccaa ttcactaaaa aaagttgcaa cacttgcatt
      301 agcaaattta ttattagttg gtgcacttac tgacaatagt gccaaagccg aatctaagaa
      361 agatgatact gatttgaagt tagttagtca taacgtttat atgttatcga ccgttttgta
      421 tccaaactgg agacttttaa cataaaatta cttatcattc aaaaagtaaa acagcataat
      481 atcaaggttt ataactttat cattatcaat aatacctcat ataaaataaa attttaggga
      541 ctttttaggg actttaaatt taaaattaca agtttaatag aaacatcaaa ataatcacat
      601 gtttgtgtgg aatgtacacc ccaaaagcta gactgaaaaa tcta
//
LOCUS       DALAGD010000236          562 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.245, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000236 DALAGD010000000
VERSION     DALAGD010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.245"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(1..312)
                     /locus_tag="O2701_002845"
                     /pseudo
     CDS             complement(1..312)
                     /locus_tag="O2701_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837136.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
     gene            complement(359..>562)
                     /locus_tag="O2701_002846"
     CDS             complement(359..>562)
                     /locus_tag="O2701_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCZ4294690.1"
                     /translation="FKNICFTIFYYLFTGFMSYGAFYLLVFENVQNLILYVSWLFMTM
                     LFMFMNMHSIIDKKVHIFLKSNK"
BASE COUNT          228 a           87 c           60 g          187 t
ORIGIN      
        1 aaatacagtg attgctagtt gtttttcaaa ctgaacatta aagaagttac cgtactttct
       61 caatataatt ccaaatagaa ctgaacttcc tgcaaccggc caaaaaacat tatttttaac
      121 aggctcgact atatttaacc tttaaaattc attttcgcta atttttgcgt attttttggg
      181 caaaaaccaa ttaatcatag ggaaaaaata agtaatccaa gtacttacta aatcgaccat
      241 gaaatattca ttattatatc taataactcg atatttagga tttttataaa tttgtctaga
      301 ttcacaaagc actgtttacc acccctaatt aacaattgaa aactaactaa atttgtaact
      361 atttattaga ctttaagaat atatgtactt ttttatctat aattgaatgc atattcataa
      421 acataaatag catagtcatg aaaagccaag aaacatataa gattaaattt tgcacatttt
      481 caaataccaa caagtaaaat gccccataag acatgaaacc agtaaatagg taataaaaaa
      541 ttgtgaaaca aatattttta aa
//
LOCUS       DALAGD010000237          544 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.246, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000237 DALAGD010000000
VERSION     DALAGD010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.246"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            123..>544
                     /locus_tag="O2701_002847"
     CDS             123..>544
                     /locus_tag="O2701_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCZ4294691.1"
                     /translation="MYKNYNLIQLTLPIETSVRIPQIDISRYVNEIVETIPDSEFDKF
                     RHHRGATSYHPKMMLKITLYAYTQSVFSGRRIEKLLHDSIRMMWLAQNQTPSYKTINR
                     IRVNPNTDALIESLFIQFHSQCLKQNLIDGTKVEANAN"
BASE COUNT          200 a           88 c           74 g          182 t
ORIGIN      
        1 aaaaaataag acactttgcc aaacttgcac ataaatgttt aattcaataa tttgaatttt
       61 ctgtgttggg tcccttcgta taatttaata aataccacta aactaaatta atgaagtgcc
      121 ttatgtataa aaattataac ttgatccaac ttacactacc aatagaaact tctgttagaa
      181 ttcctcaaat tgatatttcg cgatatgtta atgaaattgt tgaaacgata cctgatagcg
      241 aattcgataa attcagacat catcgtggcg caacatccta tcatccaaaa atgatgttaa
      301 aaatcacctt atatgcatat actcaatctg tattttctgg tcgtagaata gagaaattac
      361 ttcatgacag tattcgaatg atgtggttag ctcaaaatca aacaccttct tataaaacta
      421 ttaatcgtat tagagtgaat cctaatactg atgcgttaat tgaatcttta tttatccagt
      481 ttcatagtca atgtttaaag caaaatctta ttgatggtac aaaagtagaa gctaatgcca
      541 atag
//
LOCUS       DALAGD010000238          522 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.247, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000238 DALAGD010000000
VERSION     DALAGD010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.247"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            101..493
                     /locus_tag="O2701_002848"
     CDS             101..493
                     /locus_tag="O2701_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294692.1"
                     /translation="MQFKLKEEEIISFLELKYPEKEFEYGRLLVGQHKRDDLDVYYFG
                     DTFLMCTIISFKTFEIKETVELSYDAVNRIVLKDGWLFRKMRIETMQKVLKYGTSKLM
                     LTDFQKENYNKYIQGQKQRVIFENGHFV"
BASE COUNT          212 a           41 c           94 g          175 t
ORIGIN      
        1 caaagtatta aaaaacataa cgaaatgatg tatattgaac tatgtgattg aaaaaatttt
       61 taattaaggc ttgttaaaac ttaagagagg atgtttttaa atgcaattca aattaaaaga
      121 agaagagatt attagttttt tagaattgaa atatccagaa aaagagttcg aatatggtcg
      181 tttgttagtt ggacaacata aacgtgatga tttagatgtt tattactttg gtgatacgtt
      241 tttaatgtgc acgattattt cattcaagac atttgaaatt aaagaaacag tagaattatc
      301 atatgatgct gttaatcgta ttgtgttaaa agatggatgg ttatttagaa aaatgagaat
      361 agaaacaatg caaaaagtgt taaaatacgg tacatctaaa ttaatgttaa ctgattttca
      421 aaaagagaat tataataaat atattcaagg tcagaaacaa cgcgtgatat ttgaaaatgg
      481 ccattttgtc taattgatag tgaatataat tagagtaaga gg
//
LOCUS       DALAGD010000239          521 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.248, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000239 DALAGD010000000
VERSION     DALAGD010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.248"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(5..93)
                     /locus_tag="O2701_002849"
     tRNA            complement(5..93)
                     /locus_tag="O2701_002849"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(57..59),aa:Leu,seq:taa)
     gene            complement(101..175)
                     /locus_tag="O2701_002850"
     tRNA            complement(101..175)
                     /locus_tag="O2701_002850"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(141..143),aa:Gly,seq:gcc)
     gene            complement(179..260)
                     /locus_tag="O2701_002851"
     tRNA            complement(179..260)
                     /locus_tag="O2701_002851"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(224..226),aa:Leu,seq:tag)
     gene            complement(264..339)
                     /locus_tag="O2701_002852"
     tRNA            complement(264..339)
                     /locus_tag="O2701_002852"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(304..306),aa:Lys,seq:ttt)
     gene            complement(345..420)
                     /locus_tag="O2701_002853"
     tRNA            complement(345..420)
                     /locus_tag="O2701_002853"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Thr,seq:tgt)
     gene            complement(437..512)
                     /locus_tag="O2701_002854"
     tRNA            complement(437..512)
                     /locus_tag="O2701_002854"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(477..479),aa:Val,seq:tac)
BASE COUNT          135 a          145 c          128 g          113 t
ORIGIN      
        1 aaaatggtgc cgaggaccgg aatcgaaccg gtacggtgat ctctcaccgc aggattttaa
       61 gtcctgtgcg tctgccagtt ccgccacccc ggcaaaataa tggagcagaa gacgggattc
      121 gaacccgcga ccccaacctt ggcaaggttg tattctaccg ctgaactact tctgcatatg
      181 cgggtgaagg gagtcgaacc cccacgccgt aaggcgctag atcctaagtc tagtgcgtct
      241 gccaattccg ccacacccgc aaatggtgag ccatagagga ttcgaacctc tgaccctctg
      301 attaaaagtc agatgctcta ccaactgagc taatggctct tccatggtgc cggccagagg
      361 acttgaaccc ccaacctact gattacaagt cagttgctct accaattgag ctaggccggc
      421 aatatgtaag aataaatggt ggagaatgac gggttcgaac cgccgaccct ctgcttgtaa
      481 ggcagatgct ctcccagctg agctaattct ccgatttaaa a
//
LOCUS       DALAGD010000240          500 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.249, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000240 DALAGD010000000
VERSION     DALAGD010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.249"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
BASE COUNT          209 a           61 c           65 g          165 t
ORIGIN      
        1 cattacaaat aacaataaca aatttcaacc gataagataa aatgtcttgt cggtttttaa
       61 tataacaagt gatattttgg aaatgccaaa aatatataga ttgtttatat aaaatgattt
      121 aaaatgtttt ataacaacat attttatgca acacataaaa tgggtaacta acatcacgaa
      181 atcattgtat ttgttgttac actgatgaag cgatgtcaca aagaaatgtc gttatcaaac
      241 gaagcccgaa atacatactg atactcgaaa taatgtaacg ataaataaca atgataacga
      301 ttgattacta cgcaaaaata tatgtaaatt gacgatttaa atgcaattcg tatattttgc
      361 caatgattta cacattaaaa tgtttgaaaa ttgtcacaat tcagtctaat ttaaaaggaa
      421 gttaattaca aaattaaaaa atcatgcatc gtgtcattta gatattgaaa aagatgagtc
      481 aaatttgttt catatatttt
//
LOCUS       DALAGD010000241          485 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.250, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000241 DALAGD010000000
VERSION     DALAGD010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.250"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..149
                     /locus_tag="O2701_002855"
     CDS             <1..149
                     /locus_tag="O2701_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002484688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294693.1"
                     /translation="VTAKHYISKRLGHADVHTTLKTYSHLIKEFEDNETKFIEKNLND
                     LFCD"
     gene            complement(345..433)
                     /locus_tag="O2701_002856"
     tRNA            complement(345..433)
                     /locus_tag="O2701_002856"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(396..398),aa:Ser,seq:gga)
BASE COUNT          193 a           77 c           80 g          135 t
ORIGIN      
        1 aagtaacagc taaacattac atttcaaaac gattaggtca tgcagatgta catacaacgc
       61 ttaaaacata ttcacatttg attaaagaat ttgaagataa tgaaactaaa tttatagaaa
      121 aaaatctgaa tgatttgttt tgtgactaaa cttcaaaatc ttgtgactaa aattaggatt
      181 attcatcaaa aattagtcac attgtggcta aaaaatttta aatggatata aataaataag
      241 aagtgtaaaa atgaggataa tagctcaaat gcagatatat caagggattg agtccaaaat
      301 aaaaatagcg cttaaaatgg gtaaaaccaa tttaagcgcc tttacggaag gtaagggatt
      361 cgaacccttg agacgcttgt gacgcctaca cactttccag gcgtgctcct tcggccagct
      421 cggacaacct tcctcaacaa taaatatgta ataaaaaaac agaagcgata tttcacttct
      481 gcttg
//
LOCUS       DALAGD010000242          477 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.251, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000242 DALAGD010000000
VERSION     DALAGD010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.251"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            242..477
                     /locus_tag="O2701_002857"
                     /pseudo
     CDS             242..477
                     /locus_tag="O2701_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          192 a           72 c           66 g          147 t
ORIGIN      
        1 aataagacac tttgcccaac ttgcacataa atgtaaaatt caataaaata aattttctgt
       61 gttggatccc ttcgtataat ttaataaata ctactaaact aaattaacga ggtgccttat
      121 gtataaaaat tataacatga cccaacttac actaccaata gaaacttctg ttagaattcc
      181 tcaaaatgat aatacgcgat atgttaatga aattgttgaa acgatatctg atagcgaatt
      241 cgatgaattt atatgtccta ataacaaaag aataggtttt aaaagatatg cataccgtaa
      301 tgatagatat agttttaaac gtgacttcaa gctatatgaa tgtgatgact gttcatcatg
      361 ttctttgaga catcaatgca tgaagccaaa ttcgaaatcc aataagaaaa ttatgaagaa
      421 ttataattgg gaatacttta aagcccaaat taatcaaaag ctttctgaac cagaaac
//
LOCUS       DALAGD010000243          474 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.252, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000243 DALAGD010000000
VERSION     DALAGD010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.252"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(<1..391)
                     /locus_tag="O2701_002858"
     CDS             complement(<1..391)
                     /locus_tag="O2701_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCZ4294694.1"
                     /translation="METLLCESKVINKNPKYRIIKYDSEYLMIDLASNWIVFFFPFIN
                     WLIPKTYVKITKNDYEKLNIVKPVKNKSIGWTIFAGIVLLGGTVRRNTYLFDFQLEEL
                     IVWSSCFIGFLEIIFFYCYLNKKLTLNI"
BASE COUNT          173 a           77 c           51 g          173 t
ORIGIN      
        1 aaatatttaa tgttaatttc ttatttagat aacaataaaa aaatataatc tctaaaaacc
       61 caatgaaaca gctgctccaa acaattagtt cttctaattg aaaatcaaat aaataagtat
      121 ttcttcttac agtaccacca agtaacacaa tacccgcgaa tatggtccat cctatcgatt
      181 tatttttaac tggtttgaca atatttaatt tttcataatc attcttagtg attttgacat
      241 atgttttcgg tatgagccag ttaataaatg gaaagaagaa gacaatccaa ttacttgcca
      301 aatcaatcat taaatattca ctatcgtatt tgattattcg atatttaggg tttttattaa
      361 taactttaga ttcgcaaagc aatgtctcca catcccttta attttatgtg taatacattt
      421 ttcgatactt caaaagacat tcaaatacta tcaagttact gtcatcaaag gttt
//
LOCUS       DALAGD010000244          474 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.253, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000244 DALAGD010000000
VERSION     DALAGD010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.253"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(143..>474)
                     /locus_tag="O2701_002859"
     CDS             complement(143..>474)
                     /locus_tag="O2701_002859"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCZ4294695.1"
                     /translation="MQTTAGEIGVMSGHIPTVAALKTGFVKVKFHDGTEYIAVSDGFV
                     EVRKDKVSIIVQTAETAREIDVERAKLAKARAESHLENDDDNTDIHRAERALERANNR
                     LRVAELK"
BASE COUNT          135 a          107 c           59 g          173 t
ORIGIN      
        1 ttaatgataa agaaccacta catcataaat ctttaatggt tctttatcaa ttccttcccc
       61 cctctcatgt tttgtatctt taaatcaata taattcaaaa aagggtcgaa gatatgaaat
      121 cacatcttcg accctttatt tactatttta attcagccac acgcaaacgg ttatttgctc
      181 tctctaaagc tctttcggct ctatgaatat cagtattgtc gtcatcattt tccaagtgag
      241 actctgctct tgctttggct aatttagctc tttcaacatc aatttctctt gcagtttctg
      301 cagtctgaac aatgattgaa actttatctt ttctaacttc aacaaagcca tcgcttacag
      361 caatatattc agttccatcg tgaaatttca cttttacaaa gcctgttttt aaagcagcta
      421 cagttggaat atgtccactc atgacaccta tctcaccagc tgttgtttgc ataa
//
LOCUS       DALAGD010000245          470 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.254, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000245 DALAGD010000000
VERSION     DALAGD010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.254"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            1..72
                     /locus_tag="O2701_002860"
     tRNA            1..72
                     /locus_tag="O2701_002860"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33..35,aa:Pro,seq:tgg)
     gene            90..165
                     /locus_tag="O2701_002861"
     tRNA            90..165
                     /locus_tag="O2701_002861"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:123..125,aa:Ala,seq:tgc)
     gene            187..260
                     /locus_tag="O2701_002862"
     tRNA            187..260
                     /locus_tag="O2701_002862"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:221..223,aa:Met,seq:cat)
     gene            289..362
                     /locus_tag="O2701_002863"
     tRNA            289..362
                     /locus_tag="O2701_002863"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:323..325,aa:Ile,seq:cat)
     gene            372..461
                     /locus_tag="O2701_002864"
     tRNA            372..461
                     /locus_tag="O2701_002864"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:408..410,aa:Ser,seq:tga)
BASE COUNT          103 a          111 c          128 g          128 t
ORIGIN      
        1 ggaagtagct cagcttggta gagcacttgg tttgggacca aggggtcgca ggttcgaatc
       61 ctgtcttccc gatatactgt aattattatg ggggcttagc tcagctggga gagcgcctgc
      121 tttgcacgca ggaggtcagc ggttcgatcc cgctagtctc caccatatta tttacaaact
      181 atataaggcg gtgtagctca gctggctaga gcgtacggtt catacccgtg aggtcggggg
      241 ttcgatcccc tccaccgcca ctatttatta gttgtaaaat tatatttagg acctttagct
      301 cagttggtta gagctaacgg ctcataaccg ttcggtcgca ggttcgagtc ctgcaaggtc
      361 catataattt tggaggaata cccaagtccg gctgaaggga tcggtcttga aaaccgacag
      421 gggcttaacg gctcgcgggg gttcgaatcc ctcttcctcc gttttactaa
//
LOCUS       DALAGD010000246          439 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.256, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000246 DALAGD010000000
VERSION     DALAGD010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.256"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..393
                     /locus_tag="O2701_002865"
     CDS             <1..393
                     /locus_tag="O2701_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HCZ4294696.1"
                     /translation="GSSVIMGYMIKSQEMPIFITYDKHEDISDSTKYEDEFLSQDELK
                     WFTKSNRTLESKEVQKILSHRAKGIKMYIFVQKKDDDGIYFYYLGTAGYIEGSEKQDK
                     MPNGSNVVTMDLALDKAVRDDIYRYLTN"
BASE COUNT          184 a           44 c           82 g          129 t
ORIGIN      
        1 ggttcgtctg taatcatggg atacatgata aaaagccaag aaatgccaat atttatcact
       61 tatgataagc atgaggatat aagtgatagc acaaaatatg aagatgaatt tttaagtcaa
      121 gatgaattaa aatggtttac taaatctaat agaacgctag aatcaaaaga agttcagaaa
      181 attttgtccc atagagcaaa aggaattaaa atgtatattt ttgtacagaa aaaagatgat
      241 gatggtatat atttttatta tttaggaact gctggatata tagaaggttc agaaaagcaa
      301 gataaaatgc cgaatggttc aaacgtagta acgatggatt tagcactgga taaagctgta
      361 agagatgata tatatcgtta tctaacaaat taatgtaaac cgaaaaatga atgttagtaa
      421 agttgtattt aaacatcaa
//
LOCUS       DALAGD010000247          423 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.257, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000247 DALAGD010000000
VERSION     DALAGD010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.257"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            8..>423
                     /locus_tag="O2701_002866"
     CDS             8..>423
                     /locus_tag="O2701_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HCZ4294697.1"
                     /translation="MKKEKRLNLILTVIQQNQFNKKQQIVDYMARHFGVYYSLTTISR
                     DLQELEIYKIPVENKKYIYKKINQTNQLSAKKQLEIFSDEIIEFITLNNYVLIKTSPG
                     FAQSISYYIDQLQMKEILGIIGGNDTLMILTSSNEIA"
BASE COUNT          188 a           50 c           55 g          130 t
ORIGIN      
        1 aataaaaatg aaaaaagaaa aacgtttaaa tcttatctta actgttatac aacagaatca
       61 gtttaataaa aaacaacaaa tagtggatta catggcaaga cattttggag tttactatag
      121 cttgacaact atttctcgtg acttacaaga attagaaatt tacaaaatcc ctgttgaaaa
      181 taaaaagtat atttacaaga aaataaatca aacaaatcaa ttaagtgcaa aaaaacaatt
      241 agaaatattt agtgatgaga ttattgaatt tataacgcta aataactatg tcttaataaa
      301 aacatctcct ggctttgctc aaagtataag ttattacata gatcaattac aaatgaaaga
      361 aatattagga attattggag gtaacgatac tttgatgatt ttgacttctt caaatgaaat
      421 agc
//
LOCUS       DALAGD010000248          421 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.258, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000248 DALAGD010000000
VERSION     DALAGD010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.258"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(158..346)
                     /locus_tag="O2701_002867"
                     /pseudo
     CDS             complement(158..346)
                     /locus_tag="O2701_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          140 a           51 c           57 g          173 t
ORIGIN      
        1 tttatattaa aattttgaat tcaaaaatta ttgaaaggga agctacctta gaaattgaat
       61 ctatggccac taactgcttt tttatatcac cacttattaa aaaatgtaag aatattcctc
      121 agtgctaagt gaaggaggtt ctagttttat ttaatattca tattgatagg taatggttaa
      181 taattgcctg taaattattg ttaaatatcg ttgttatagt atcatcattc ataaaatttt
      241 tatagatttt atcaagtatt tcctcatctt caatagctgt aaagcgatgt actagtcttt
      301 ttatgagcta tatataattt tgcgaagcta aactgtttgt aattattcac caatcaggtt
      361 atgtagtgtc acggttcata ggtaattctc tttttaaaat catctaatat tttatataat
      421 t
//
LOCUS       DALAGD010000249          419 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.259, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000249 DALAGD010000000
VERSION     DALAGD010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.259"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            18..>419
                     /gene="lpl8"
                     /locus_tag="O2701_002868"
     CDS             18..>419
                     /gene="lpl8"
                     /locus_tag="O2701_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl8"
                     /protein_id="HCZ4294698.1"
                     /translation="MGYIKRMALYMSVFLLIIFIVGCRNMKDEQKKEEQTNKTDSKEE
                     QIKKSFEKTLDMYPIKNLEELYDKEGYRDGEFKKGDKGMWTIYTDFAKSNKQGGLSNE
                     GMVLYLDRNTRTAKGHYFVKTFYNKGKFPDRK"
BASE COUNT          180 a           42 c           90 g          107 t
ORIGIN      
        1 cagagagatg tgaaatgatg gggtatataa aaagaatggc tttatacatg agtgtatttc
       61 ttttaatcat ttttattgtt ggatgtcgaa atatgaaaga tgaacagaaa aaagaagaac
      121 aaacaaataa aacagattca aaagaagaac aaatcaaaaa gagttttgag aaaacattag
      181 atatgtatcc aattaagaat ctcgaggagt tatacgacaa agaaggatac cgagatggcg
      241 aatttaaaaa gggtgataaa gggatgtgga cgatatatac agatttcgcc aaaagtaata
      301 aacaaggtgg attgagtaat gaaggtatgg tcttatactt agatagaaat acacggactg
      361 caaagggaca ttattttgtt aagacattct ataataaggg caaattccca gatagaaaa
//
LOCUS       DALAGD010000250          405 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.260, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000250 DALAGD010000000
VERSION     DALAGD010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.260"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..182
                     /locus_tag="O2701_002869"
     CDS             <1..182
                     /locus_tag="O2701_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294699.1"
                     /translation="SNGKGSVTFNKKDAEVIDFSLSEIENEEYFMLYRNKSFSVVRDF
                     IEKQEFPENYKIAWY"
     gene            252..405
                     /locus_tag="O2701_002870"
                     /pseudo
     CDS             252..405
                     /locus_tag="O2701_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463904.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
BASE COUNT          164 a           44 c           68 g          129 t
ORIGIN      
        1 taagtaacgg taaaggttca gtaactttta ataaaaaaga tgcagaggtt atagatttct
       61 cattatctga aatagaaaat gaagaatatt ttatgttata tcgtaataag tctttttctg
      121 tagtaagaga ctttatcgag aagcaagaat ttcccgaaaa ttataaaata gcatggtatt
      181 aaagcatcct ttctacacag ataaagagaa agcgatgcta tttttatatg cttttttcat
      241 tattcgttga aaaagaagag tcacaaaagt taacatattt aaaagaactt ggtgaagatg
      301 gcgaatataa atatgttgcc aaaatagata gtaaaacatc taaattatgt cattcactca
      361 acggaaaaat atttaaagtt aaagatatga taccaggtgt gaatg
//
LOCUS       DALAGD010000251          400 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.261, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000251 DALAGD010000000
VERSION     DALAGD010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.261"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(93..308)
                     /locus_tag="O2701_002871"
     CDS             complement(93..308)
                     /locus_tag="O2701_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294700.1"
                     /translation="MSINIDPEKFAELVVMSNPSKFEDAEDIAKESLKLYINAYRLAE
                     RYSTIATNCYDTAEVIKELKKTDLQLK"
BASE COUNT          129 a           58 c           49 g          164 t
ORIGIN      
        1 agatgattgc ttttctatcc aataaaaaag aagagaagat gtaacacatc ttctcttctg
       61 caatattaat taggatttat ttctaagttg agttatttta attgtaaatc tgttttcttt
      121 aattctttta taacttctgc agtatcataa caatttgttg caattgttga atatctctct
      181 gctaaacgat atgcattaat gtaaagcttt aaactttctt tagctatatc ctctgcatct
      241 tcgaattttg atgggttaga cataaccact aattctgcaa atttttctgg atcaatatta
      301 atagacatgt atttatttac aactcctatt tattttgatg tcttaatact aacatattga
      361 agttttcaga caaagtaatg tctctctata attgaagaaa
//
LOCUS       DALAGD010000252          389 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.263, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000252 DALAGD010000000
VERSION     DALAGD010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.263"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            133..237
                     /locus_tag="O2701_002872"
     CDS             133..237
                     /locus_tag="O2701_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294701.1"
                     /translation="MYFKNSSLNYIPTTFGGEPKKKHFPKMESASSEP"
     gene            complement(228..300)
                     /locus_tag="O2701_002873"
     tRNA            complement(228..300)
                     /locus_tag="O2701_002873"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(265..267),aa:Lys,seq:ttt)
     gene            complement(304..378)
                     /locus_tag="O2701_002874"
     tRNA            complement(304..378)
                     /locus_tag="O2701_002874"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(344..346),aa:Gln,seq:ttg)
BASE COUNT          138 a           77 c           75 g           99 t
ORIGIN      
        1 tcttttggtt acagaaattt caacaacttt aaagcacgta taatgatgat tttcagcttg
       61 tacaaaggag aaaaaaagaa gacaaccaag cccaataatg gactggccgc ctaataataa
      121 aagctctaaa agttgtattt taaaaatagt tctttaaatt atatacccac cacatttggt
      181 ggagaaccta aaaaaaagca cttcccaaaa atggaaagtg caagtagtga gccatagagg
      241 attcgaacct ctgaccctct gattaaaagt cagatgctct accaactgag ctaatggctc
      301 taatggctgg gctagctgga ttcgaaccaa cgagtgacgg agtcaaagtc cgttgcctta
      361 ccgcttggct atagcccaat atatagatg
//
LOCUS       DALAGD010000253          384 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.264, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000253 DALAGD010000000
VERSION     DALAGD010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.264"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..>384
                     /locus_tag="O2701_002875"
     CDS             <1..>384
                     /locus_tag="O2701_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="E domain-containing protein"
                     /protein_id="HCZ4294702.1"
                     /translation="KNPLTGEIISKGESKEEITKDPINELTEYGPETITPGHRDEFDP
                     KLPTGEKEEVPGKPGIKNPETGDVVRPPVDSVTKYGPVKGDSIVEKEEIPFKKERKFN
                     PDLAPGTEKVTREGQKGEKTITTPTL"
BASE COUNT          174 a           61 c           83 g           66 t
ORIGIN      
        1 aaaaaatcca ttaactggag aaattattag taaaggtgaa tcgaaagaag aaatcacaaa
       61 agatccgatt aatgaattaa cagaatacgg accagaaacg ataacaccag gtcatcgaga
      121 cgaatttgat ccgaagttac caacaggaga gaaagaggaa gttccaggta aaccaggaat
      181 taagaatcca gaaacaggag atgtagttag accaccggtc gatagcgtaa caaaatatgg
      241 acctgtaaaa ggagactcga ttgtagaaaa agaagaaatt ccattcaaga aagaacgtaa
      301 atttaatcct gatttagcac cagggacaga aaaagtaaca agagaaggac aaaaaggtga
      361 gaagacaata acgacgccaa cact
//
LOCUS       DALAGD010000254          379 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.265, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000254 DALAGD010000000
VERSION     DALAGD010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.265"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
BASE COUNT          151 a           47 c           80 g          101 t
ORIGIN      
        1 aaaagcaaac acaaagaaaa atggcttggc gaagtgaaaa cgtttgaatc tgacgaaacg
       61 agaaaagaac gcaacgagtt tagtagagct aaatgagtaa gtgagagccg aaggagagga
      121 aagaagcaag cgattgtcac aagtcaagaa aggttcttag cgaggatggt agccaactta
      181 cgttccgcta gagtagaact ggaaatgata atttaataat gtacactttc gattgtctaa
      241 gtatgtacaa ctttaatttt gtgtttatat aaatttaaaa tgatatcatc gaaaacaaaa
      301 tattgtataa atagagaaga gcagtaagac ggtatctaat tgaaaatgat cttactgctc
      361 ttttatatac tttattgaa
//
LOCUS       DALAGD010000255          377 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.266, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000255 DALAGD010000000
VERSION     DALAGD010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.266"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            206..377
                     /locus_tag="O2701_002876"
                     /pseudo
     CDS             206..377
                     /locus_tag="O2701_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458050.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          149 a           61 c           74 g           93 t
ORIGIN      
        1 ggggccccaa caaagagaaa ttggattccc aatttctaca gacaatgcaa gttggggtgg
       61 gacggcgaaa taaattttgc gaaaatatca tttctgtccc actccccaga aaaggttcta
      121 ccattgtcaa aaaaatgcat ctctacatgt tagagtaaat attggtcagc caaccaaaat
      181 aatcaacacg aggagatgct atttaatgtc atctgacaca aacagtttag cacatacgaa
      241 atggaattgt aagtaacata ttgtctttgc acctaaatac agaagacaag cgatatatgg
      301 aaaaataaaa aaagatatag ggattatatt acgtcaatta tatgaaagaa aaggtgtaga
      361 gataattgaa gcagagg
//
LOCUS       DALAGD010000256          368 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.267, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000256 DALAGD010000000
VERSION     DALAGD010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.267"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
BASE COUNT          137 a           43 c           55 g          133 t
ORIGIN      
        1 ttcagaattt ttagtatttt cagatattta ttcttaagta tcttattatt acttcttttt
       61 aacgctagtg gaataattaa taataattgt atttaaatgc aatgctgttg ttctttcata
      121 cttacaagca agtcataaga aatgagaaat taaattctta tatgatgaat aaatgatgac
      181 tgcagaagaa attgctaaaa aaggagtgga gtgaggcatt ctactgttca tattgttaac
      241 aaatatcaaa atgataggaa cttgaagaat ctaagactgt taaaatcgag acaataagaa
      301 actcgttcca aatttgaatc ctataatgaa ttcgtctcat gaagaagtta tttacttttc
      361 attagact
//
LOCUS       DALAGD010000257          354 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.268, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000257 DALAGD010000000
VERSION     DALAGD010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.268"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(<1..162)
                     /locus_tag="O2701_002877"
     CDS             complement(<1..162)
                     /locus_tag="O2701_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294703.1"
                     /translation="MTNPTRTKRCGGDVLYGIDNHFQLLLYISENHCQLETKTFFEYF
                     LRIVNKTHDY"
BASE COUNT          120 a           63 c           47 g          124 t
ORIGIN      
        1 gtaatcatgc gttttattta ctattcttaa aaaatattca aaaaaagttt tagtttctaa
       61 ttgacaatga ttctcactaa tgtataatag tagttgaaaa tgattatcaa taccatatag
      121 aacatccccc ccacaacgtt tcgttcttgt tggattggtc attttcaaat attccccttt
      181 tatatgcccg taaaagacaa tatacgttat aacaacgttt tataaaagca gtaaaccctt
      241 acgacacttt aggtttactg cttttgtttt ttgtaaaaag aaaaccatac gctatgcgta
      301 tggttcagaa aaggttctac cattgtcaca aaaaatgcat ctctacgtgc taga
//
LOCUS       DALAGD010000258          327 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.269, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000258 DALAGD010000000
VERSION     DALAGD010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.269"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
BASE COUNT          148 a           51 c           42 g           86 t
ORIGIN      
        1 atgaaaatta ataaattatc aaattgacat accttattgt atttatagaa aaacaaaaaa
       61 aggtaagcac cgaaatgctt acctacttcc cataaacaat ataacacata tacattgatt
      121 tggaaagcgc aaaaataaat ttaatatact agcccgaagg ggagcaatac ataaaaaatg
      181 aaaggcgctc cttgaaaacg cccaaggtaa tattaacata aaatggctac tattgcatta
      241 tctaatttta tttaattaaa acaaatatat atagcacaaa aaaactagcc cgaaggctag
      301 cttatgaata gatgaaaatt tgaacac
//
LOCUS       DALAGD010000259          297 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.270, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000259 DALAGD010000000
VERSION     DALAGD010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.270"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..174
                     /locus_tag="O2701_002878"
     CDS             <1..174
                     /locus_tag="O2701_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HCZ4294704.1"
                     /translation="KRYDIPTNKAPKLLLKGTGNLKGSSVGYKKIEFTFLENKNENIY
                     FTDSLHLEPSEDK"
BASE COUNT          120 a           34 c           52 g           91 t
ORIGIN      
        1 aaaagatatg acataccaac aaataaagcg ccgaagctgt tgttgaaagg tacagggaat
       61 ttaaaaggtt catcagttgg atataaaaaa attgaattta cttttttaga gaataaaaat
      121 gaaaatattt actttactga tagtctacat cttgaaccga gcgaggataa ataatcatct
      181 tttaaccaga atatgaaatc aatctaggac aacctaataa aaggagagag ttgtaagatg
      241 atgaaacgat taaacaaatt agtgttaggc attatttttc tgtttttagt cattagt
//
LOCUS       DALAGD010000260          289 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.271, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000260 DALAGD010000000
VERSION     DALAGD010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.271"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
BASE COUNT          113 a           35 c           36 g          105 t
ORIGIN      
        1 attctgaaaa atgggtataa atagtagaag aagttaactt ggaagagtta agctataaca
       61 aagaatctct ttagacacac attgaatatc gaaacattta attgcgctaa atcgtttcat
      121 taaataaatt accttgtatt gtcgattaaa ttaaggtaaa ttataaaaaa tgctgatatt
      181 tttgactaaa ccaaatgcta acccagaaat acaatcactg tgtctaatga ataatttgtt
      241 ttataaacac ttttttgttt acttctcatt tttaattagt tataattaa
//
LOCUS       DALAGD010000261          286 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.272, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000261 DALAGD010000000
VERSION     DALAGD010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.272"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            198..>286
                     /locus_tag="O2701_002879"
     CDS             198..>286
                     /locus_tag="O2701_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCZ4294705.1"
                     /translation="MNDLKSKSNIKLMKRVLTTYELRKYLKKY"
BASE COUNT          133 a           29 c           38 g           86 t
ORIGIN      
        1 aactaaaaat cacagaataa caaatgacaa attcatttat tatccaaaaa taaaaacaat
       61 tgaacaaaat caaaatatag tgttatgatt catttgtgtt tatagctagc accacaaact
      121 aaatatattt ggagttatta ctagttaaag acaaatatta tttgacgtga caattaaaag
      181 taagggatga agacttgatg aatgatttga aaagcaagag caatattaaa ttaatgaaaa
      241 gagtattaac tacatatgag ctgcgtaaat atttaaaaaa atattt
//
LOCUS       DALAGD010000262          282 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.273, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000262 DALAGD010000000
VERSION     DALAGD010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.273"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            15..>282
                     /locus_tag="O2701_002880"
     CDS             15..>282
                     /locus_tag="O2701_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCZ4294706.1"
                     /translation="MKHFAQLTLPIETAVRIPQNDISRYVNEIVKKIADSEFDEFRHH
                     RGATSYHLKMMLKITSYSYTQSEFSGRRIEKLLHNSIRMMWLAQD"
BASE COUNT          109 a           50 c           39 g           84 t
ORIGIN      
        1 aaatacttaa aaaaataaaa cactttgccc aacttacact accaatagaa actgctgtta
       61 gaattcctca aaatgatatt tcgcgatatg ttaatgaaat tgttaaaaag atagctgata
      121 gcgaattcga tgaattcaga catcatcgtg gcgcaacatc ctatcatcta aaaatgatgt
      181 taaaaatcac ctcatattca tatactcaat ctgaattttc tggccgtaga atagaaaaat
      241 tacttcataa cagtattcga atgatgtggt tagctcaaga tc
//
LOCUS       DALAGD010000263          219 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.denovo.277, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000263 DALAGD010000000
VERSION     DALAGD010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..219
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.denovo.277"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
BASE COUNT           73 a           37 c           23 g           86 t
ORIGIN      
        1 agtgttcttt cgaacactag cgattatttc ttatgaattc aagcttattt aaaactcttt
       61 attcactcgg ttttgcttgg taaaatctat attttactta cttatctagt tttcaatgta
      121 caatttcttt ttagtcaagc gctcgcataa gcaatatcac tttaaccaaa aaatatttga
      181 atgttaaata aacattcaaa actgaataca atatgtcac
//
LOCUS       DALAGD010000264        19636 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.guided.059, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000264 DALAGD010000000
VERSION     DALAGD010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19636
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.guided.059"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            413..1285
                     /locus_tag="O2701_002881"
     CDS             413..1285
                     /locus_tag="O2701_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCZ4294707.1"
                     /translation="MSNVLTEIDSQYPYMTKNEKKIAKFILNSPQKVIKMRSQDLASL
                     LDISTSSVIRFSKKITDGGFHDLKINISKYVPKASSIYNVELMNNESTESLRTKLHTR
                     TTRALNHANNELNDKTIDQICHCLKRSETIFIYGFGASFVVATDLYQKLSRIGLNIQL
                     VQETHIFATLLATHNSNDSVILITNNGTQSEMQSMVKVIDDYHIPIITITSTRDNPVA
                     QASNIVLTYGKTDENEMHMGATTSLFAQMFTIDILYYRYVALNYHASLDFITQSKMAL
                     DNYRKHLSNINFKH"
     gene            complement(1672..3051)
                     /locus_tag="O2701_002882"
     CDS             complement(1672..3051)
                     /locus_tag="O2701_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCZ4294708.1"
                     /translation="MENNELQRGLSARQIQMIALGGTIGVGLFMGATSTIKWTGPSVI
                     LAYLIAGIFLFLIMRAMGEMIYLNPTTGSFATFASDYIHPAAGYMTAWSNIFQWIVVG
                     MSEVIAVGEYMKFWFPELPTWIPGVIAILLLMAANLFSVKAFGEFEFWFALIKVVTII
                     LMIIAGFGLIFFGFGNGGHAVGISNLWTNGGFMPNGIVGFFFALSIVIGSYQGVELIG
                     ITAGETKDPQKNIVKAVNGVIWRILIFYLGAIFVIVSVYPWNQLGDIGSPFVATFAKI
                     GITFAAGLINFVVLTAAMSGCNSGIFSASRMIYTLAHKGEMPKIFTKIMRNGVPLYTV
                     VAVSLGILIGALLNVILPLYIDGAKSIFVYVYSASILPGMIPWFMILFSHLRFRKLHP
                     EELEGHPFKMPGGAVTNYLTILFLILVLVGMVFNVETRISVLIGVIFLTIVTIYYFIR
                     YNKNNVKAK"
     gene            3331..3687
                     /locus_tag="O2701_002883"
     CDS             3331..3687
                     /locus_tag="O2701_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294709.1"
                     /translation="MLKQILLRATKISVLFAVAFFIINYIGMEKPDILYLVGRTIIAT
                     LAFILICLTVFTIINSPERKIKLGTTLPIALIIGIIFGAIFLTVQIGVITGLIIGVIA
                     TFIWELIEKNKGGRSS"
     gene            3684..3869
                     /locus_tag="O2701_002884"
     CDS             3684..3869
                     /locus_tag="O2701_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294710.1"
                     /translation="MTTALIGVIVLILLIISFVPNYRAMKSAKEQGSNATRATIMVGI
                     DIILIVLIIVTLLLKYM"
     gene            complement(3965..4156)
                     /locus_tag="O2701_002885"
     CDS             complement(3965..4156)
                     /locus_tag="O2701_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294711.1"
                     /translation="MMGYIILFFLAGPVILGVGNLVIGPIFNKQTPFRVQVRSFVVGS
                     MIYLILATIGYFLLLQGKL"
     gene            complement(4167..4802)
                     /locus_tag="O2701_002886"
     CDS             complement(4167..4802)
                     /locus_tag="O2701_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCZ4294712.1"
                     /translation="MYRAVIFDFDGTIIDTEQHLFNVINKHLEMHNADPISIDFYRSS
                     IGGAATDLHDHLIKAIGSENKDKLYEEHHLTSTTLPMIDTIKSLMAFLKQRHIPMAIA
                     TSSVKAEIMPTFKALGLDDYIEVVVGREDVEQVKPDPELYLSAVQQLNYMPTQCLAIE
                     DSVNGATAAIAAGLDVIVNTNKMTSAQDFSNVDYVAKDIDYDQIVARFFTK"
     gene            complement(4877..5794)
                     /locus_tag="O2701_002887"
     CDS             complement(4877..5794)
                     /locus_tag="O2701_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HCZ4294713.1"
                     /translation="MFQKLSIFATKSFLVWMLVAAVIGFIFPQHVATLGKWVPYLLGI
                     VMLGMGLTITPNDFKMVFKAPRAVIIGVCLQFSIMPTLAFIIAKSFHLPPDIAVGVIL
                     VGCCPGGTSSNVMSYLAKANVALSVSITTVSTLLAPFVTPALIYLFANEWLEVSFVSM
                     LWSVVQVVLIPIALGIVLQIINRKIAEKASTALPIISVVAISLILAIVVGGSKHQILT
                     TGLLIFLVVILHNVLGYTIGYWLARLLKLDRQDQKAVSIEVGMQNSGLAVSLAALHFN
                     PIAAVPGAVFSFIHNITGPILAKYWSKKL"
     gene            6062..6592
                     /locus_tag="O2701_002888"
     CDS             6062..6592
                     /locus_tag="O2701_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294714.1"
                     /translation="MLIDKFETYIINIAGLNDRTTRKKLSKLCKSVQFCDALQFSINK
                     QFNQYVLEISLPKQQLPYFISFLSFHQYSIFQVLSPKKINELLDSDNLYQSAKRFDIN
                     IDGLQDAFIKDKVIDIMNMFQNHTDITYTLNKSHAHIICTPEIFAKLLHTIATRNIDI
                     LSANYRSSSMSKARIS"
     gene            complement(6658..8262)
                     /locus_tag="O2701_002889"
     CDS             complement(6658..8262)
                     /locus_tag="O2701_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCZ4294715.1"
                     /translation="MNAIKRFGSAMIVPVLMFAFFGIVLGFATLFKNPTIMGSLADQH
                     TFWFKFWSVIESGGWVIFTHMEVVFVVGLPLSLAKKAPGHAALAALMGYLMFNTFINA
                     ILTQWPHTFGANLEKGVENVPGLKSIAGIATLDTNILGGIIISAIITWIHNRYYSKRL
                     PEMVGVFQGLTFVVTISFFVMLPLAAITCVIWPTVQHGIGSMQHFIIASGYIGVWLYH
                     FLERVLIPTGLHHFIYAPIEVGPVVVNHGLKAEWLQHLNEFAKSTKPLKEQFPYGFML
                     QGNGKVFGCLGIALAMYATTPKENRKKVAALLIPATLTAVVVGITEPLEFTFLFIAPY
                     LFVLHAVLAASMDTLMYAFGVVGNMGGGLLDFISTNWLPLGKEHWGTYVAQVIIGLIF
                     VAIYFFLFRFLILKFDIPLPGRKKTEEEVKLFSKQDYKNKKGDSVDSKRASSGNEYED
                     KAAYYLDGLGGKENIKDVTNCTTRLRLTVYDESKVADTEYFTHQQMAHGLVKSGKSIQ
                     VVVGMTVPQVREAFEQMVEDQSSEDK"
     gene            8592..9356
                     /locus_tag="O2701_002890"
     CDS             8592..9356
                     /locus_tag="O2701_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCZ4294716.1"
                     /translation="MFLDEHINRNFDKLNDNDLHIAHFINTHIDECKNMKIQDLAQFT
                     HASNATIHRFTRKLGFDGYSDFKSYLKFESNKTHQLPSNSIDSFKQEIDSTFSYLERV
                     DYQLLTKKMHTASTIYLYGTGRAQLNVASEAQRILLTLHNHVIVLHDEHELKMILNKS
                     EPNDLFFIISLSGETHELLEVTNLLQLRQKYFISVTTMKDNTLAQKANYNVYVSSNTF
                     YLTDGTDYSSFISYHIFFETLVRKYNEYLLHNDISN"
     gene            complement(9414..9920)
                     /locus_tag="O2701_002891"
     CDS             complement(9414..9920)
                     /locus_tag="O2701_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HCZ4294717.1"
                     /translation="MAKFNVENEHVEVEIEKLYKFSPELVYEAWTKKDLLKQWFMTSA
                     RTNKEIEADVKEGGKYRIVDQQRNGKVNVIEGIYESLVMDEYVKMTIGMPGLSETQDV
                     IEVEFFERETGGTQMLFYYRSLVEKERRFTNLEYKQKKKEYHDAMVHGFELMFDKMYH
                     VIETSTQQ"
     gene            complement(10087..11400)
                     /locus_tag="O2701_002892"
     CDS             complement(10087..11400)
                     /locus_tag="O2701_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HCZ4294718.1"
                     /translation="MEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLNVAITIT
                     VIVALLMNRKESFAKKVEVFTKGAGHSNIVLMMLIFILAGAFSNTAEKMGGVKSTVNL
                     GLSLIPENLIIVGLFVICMFVSISMGTSVGTVAAIAPVGYGFAQATDVPTALAMATVV
                     GGAMFGDNLSMISDTTIAAVRTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISL
                     NHAKNYDYNLIKVVPYILVLVLALLGVNVIIVLIGGTLLAGIIGLIDGSFGWMGLLDA
                     VSKGIISMEDIAMIALLIGGLVGIIQHNGGIEWLLQFVRSKVKSKRGAELGIASLVSV
                     ADIATANNTISIIMSGPLAKNIADEYDVDSRKSASILDIFGGCFQGLLPYSPQVISAA
                     GVAGISPLMLFPYSIYPILLGVCGLIAILFNFPKLKKNTSQEMKH"
     regulatory      complement(11477..11572)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(11625..12194)
                     /locus_tag="O2701_002893"
     CDS             complement(11625..12194)
                     /locus_tag="O2701_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294719.1"
                     /translation="MALIVSRAILFLILALAFGIATVFPEQKLPLYIVFTLFVAVAIV
                     SNVWFTHQNVKKHIIKFNKYVAYFLSFVSGLLYIGFVIYTLTIGSYDVLPIIIFAISI
                     VLVQILENILGISIKNTRLSNKQLKVISKQRVQQFKVISLILVSVSIVFIFIALMLKW
                     WLLLVISIIVLIIITVAMIVYEKYYTTPD"
     gene            complement(12681..13562)
                     /locus_tag="O2701_002894"
     CDS             complement(12681..13562)
                     /locus_tag="O2701_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCZ4294720.1"
                     /translation="MAAQDPRTKFKTTDYEKQEQEVPGLQSEMTPAPDCGETSYQGHQ
                     RLQGYKILVTGGDSAIGRAAAIAYAKEGADVAINYLPSEEQDAQEVRQVIEESGQKAV
                     LIPGDIRDEQFNYDLVEQAYQQLGGLDNVTLVAGHQQYHDDIHGFTTEAFTETFETNV
                     YPLFWTVQKALEYLKPGASITTTSSVQGYNPSPILHDYAASKAAIISLTKSFSEELGP
                     KGIRVNCVAPGPFWSPLQISGGQPQSKIPTFGQKTPLGRAGQPVELCGTYVLLASEES
                     SYTTGQVFGVSGGVQID"
     gene            complement(13673..14794)
                     /locus_tag="O2701_002895"
     CDS             complement(13673..14794)
                     /locus_tag="O2701_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HCZ4294721.1"
                     /translation="MGLKDQAVTWRRYFHQFPELSDKEFKTTQKIKDILTEHHIRILD
                     LPLATGLVAEVGQGLSCIAVRADIDALPIQELVEQDFKSENEGVMHACGHDIHMASIL
                     ATAVKLKEIEGTLTGRVKFIFQSAEELGHGAFKIIETHALKDVQAVLGFHNDPSRSVG
                     TFAIKTGAITSAVDRFEFHIKGVGGHAAKPEQCNDPVIVLAQLINSIQSIVSRNLSAF
                     DEAVVTIGQISCGNTWNVIADHAYVQGTVRSFDPVVRKLVETRLQDIADGLAQAYNMK
                     INLNYTHLPGAVMNDEALTHKAIAVAQHVGYKVEMMEQPLTIGEDFSGYSQHFPSVFA
                     LIGSHSEYDLHHPQYKPDERILEKVPEYFVEFVKRLLHE"
     gene            complement(15071..16309)
                     /locus_tag="O2701_002896"
     CDS             complement(15071..16309)
                     /locus_tag="O2701_002896"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HCZ4294722.1"
                     /translation="MNDLIINHIAELILPRSTDKPLKGKELDELNVVKNGTVVIKDGK
                     IVYAGTHTDDYDATETIDASGKVVSPALVDAHTHLTFGGSREHEMSLKRQGKSYLEIL
                     EMGGGILSTVNATRETSEDDLFKKAEHDLLTMIKHGVLAVESKSGYGLDRENELKQLK
                     VSNRLAEKYDLDMKHTFLGPHAVPKEASSNEAFLEEMIALLPEVKQYADFADIFCETG
                     VFTIEQSQHYMQKAKEAGFKVKIHADEIDPLGGLELAIDEQAISADHLVASSDKGKEK
                     LRNSDTVAVLLPATTFYLGKEDYADARGMLDNNGAIALATDYNPGSSVTNNLQLVMAI
                     AALKLKLSPNEVWNAVTVNAAKAIDINAGTINTGDKANLVIWDAPNHEYIPYHFGINH
                     AEKVIKDGKVIVDNTVSFKA"
     gene            complement(16309..17970)
                     /gene="hutU"
                     /locus_tag="O2701_002897"
     CDS             complement(16309..17970)
                     /gene="hutU"
                     /locus_tag="O2701_002897"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HCZ4294723.1"
                     /translation="MRKIQAKKGLSIECKGWEQEAVLRMLYNNLDPEVAERPEDLVVY
                     GGIGKAARNWEAFEAIEKTLRELESDETMLVQSGKPVAVFKTHEEAPRVLISNSVLVP
                     EWANWDHFNELDKKGLIMYGQMTAGSWIYIGSQGIVQGTYETFAELGNQHFNGDLAGT
                     VTLTAGLGGMGGAQPLAITMNHGVAICVDVDETRVDKRIDTKYCDVKTADLDEALKLA
                     EEAKERGEGLSIGLVGNAVDIHQAILEKGFKIDIITDQTSAHDPLNGYVPQGYSVEEA
                     KVLREKDPKKYVELSQASMAKHVELMLEFQKRGAVAFDYGNNIRQVAFNNGVKNAFDF
                     PGFVPAYIRPLFCEGKGPFRFAALSGDPKDIERADEEMRKLFPENEKLLRWLDLAEEK
                     ISYQGLPSRIAWLGYGERAKMGLALNRLVRDGEISAPIVIGRDHLDAGSVASPNRETE
                     SMKDGSDAVGDWAVLNALINTAAGGSWISFHHGGGVGMGYSLHAGMVVVADGSERAER
                     RLERVLTTDPGMGVARHVDAGYDIAIQTAKEKGIHIPMIDKAGDK"
     gene            18130..19014
                     /locus_tag="O2701_002898"
     CDS             18130..19014
                     /locus_tag="O2701_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCZ4294724.1"
                     /translation="MKIIQLEYFLAIVKYNSFTKAAQFLHISQPSLTATIKKMEADLG
                     YDLFTRSTKDIKITEKGIQFYRYASELVQQYRSTMEKMYDLSVTSEPRIKIGTLESTN
                     QWIANLIRKHHSDYPEQQYRLYEIHDKHQSIEQLLNFNIHLAITNEKITHEDIRSIPL
                     YEESYILLAPKETFKNQNWVDVENLPLILPNKNSQVRKHLDDYFNRRNIRPNVVVETD
                     RFESAVGFVHLGLGYAIIPRFYYQSFHTSNLEYKKIRPNLGRKIYINYHKKRKHSEQV
                     HTFVQQCQDYLYGLLEAL"
     gene            19136..19555
                     /locus_tag="O2701_002899"
     CDS             19136..19555
                     /locus_tag="O2701_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosB1/FosB3 family fosfomycin resistance
                     bacillithiol transferase"
                     /protein_id="HCZ4294725.1"
                     /translation="MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFELAG
                     LWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQ
                     SIYFTDPDGHKLELHTGTLENRLNYYKEAKPHMTFYK"
BASE COUNT         6599 a         3813 c         2744 g         6480 t
ORIGIN      
        1 tatttattaa aattcacttt attacataaa ttatacaatt aaaaagtttc ttcaaattgt
       61 aaagatgcat taatcgagtt ataatcataa tgattaagat ggtagattta gtcttatata
      121 actacattta ctaagattta aatagatgag tacataatgt ataaattggg aatatacagt
      181 atgacttacg gtaatatatc aatatattga tatattattt gcacatggga gtatgctatt
      241 ttttatctat acatcttaaa aaaatttaca gttttacaat tcaatatcat taaacaaaca
      301 acatattcag cttatttttc attctcttac gttacaagtt gtaaatacca tcgcgttaaa
      361 caacaatagc caacgctaat aaaatatatt gcgttaaagt ggagtgatta atatgtcaaa
      421 cgtactaaca gaaatagata gtcaatatcc atatatgact aaaaatgaaa aaaagatagc
      481 taaatttata ctaaattcac ctcaaaaggt gattaaaatg aggtctcaag acttagcaag
      541 cttattagat attagtacat catcagtcat tcgattcagc aagaaaatta ctgatggcgg
      601 ttttcacgac ttgaaaataa atatatctaa atatgttcct aaagcatcat caatttacaa
      661 tgttgaatta atgaataacg aaagcacaga atctttaaga actaaactcc atacacgtac
      721 tacacgcgca cttaatcatg caaataacga attaaatgat aaaactattg atcaaatatg
      781 tcattgttta aaacgttctg agacgatttt tatatatggt tttggcgcat cttttgtagt
      841 tgctaccgac ttataccaaa agttatcaag aataggtctt aatattcaac ttgttcaaga
      901 aacacatatt tttgctacat tattagcaac tcacaattcg aacgacagtg ttattcttat
      961 tactaacaat ggtacgcaaa gtgaaatgca atcaatggtt aaagttattg atgactacca
     1021 tatacctata attacaatta ctagtacaag ggataatccc gtagcacagg catcaaatat
     1081 tgttttaact tatgggaaaa ctgatgaaaa tgagatgcat atgggagcaa caacttcatt
     1141 atttgctcaa atgttcacta ttgatatttt atattatcgt tatgttgcat taaattatca
     1201 cgcatcatta gatttcataa cacaatctaa aatggcttta gataactatc gtaaacattt
     1261 atcaaatatt aactttaaac attaacagtc ttatataaat tctttaatca tgtcatataa
     1321 taatttatgc atcattttca aagcttacat gtcttttatc ttgtataaca tacgcatatg
     1381 accaatgatt tgataaggcc attcaatttc ttatattaaa aacaaacgca gttgattaac
     1441 ggattcattc catatatcaa ttgcgtttgt tattttaata atgctatttt ttattttaaa
     1501 gcccacagta aaaactaacg ataatattgc cacttataag tttttcacac tacaacttcg
     1561 atttaagcta tatttataaa aaaatgcgcc tactcataac attctttggc tatttaactt
     1621 taataagcta gggccaagtt tgtcataaga taagcgcaca ttataatcat actattttgc
     1681 ttttacatta tttttattat atctaataaa ataataaatc gttacaatcg ttaagaagat
     1741 gacaccaata agcactgata ttctagtttc aacattaaat accataccaa ccaatactaa
     1801 tattaagaat aagattgtta aatagttagt tactgcgcca ccaggcattt tgaatgggtg
     1861 accttccaat tcttcagggt gtaattttct aaaacgtaaa tgactgaata aaatcataaa
     1921 ccaaggaatc atacctggta aaatagacgc actataaacg tatacgaaaa tactcttcgc
     1981 tccatcgata tataaaggta aaattacgtt taataaagca ccaattaaaa taccaagaga
     2041 tactgctaca acagtgtaca atggtacacc atttctcatg attttagtaa atattttcgg
     2101 catttcacct ttatgagcaa gtgtataaat catacgactc gcactgaaaa tacctgagtt
     2161 acatcctgac attgctgcag ttaatacaac aaagttaatt aatccagctg caaatgtaat
     2221 accgatttta gcaaatgttg caacaaacgg acttccaatg tctcctaatt gattccaagg
     2281 atacactgaa acaataacaa aaatagcgcc taaatagaaa attaaaattc tccagataac
     2341 accattcact gctttaacga tatttttctg aggatcttta gtttcacctg ccgtaatacc
     2401 tatcagttcc acaccttggt atgaaccaat tacaattgat aatgcaaaga agaaaccaac
     2461 aataccattt ggcataaatc cgccatttgt ccatagatta gaaataccta ccgcatggcc
     2521 accatttcca aaaccaaaga aaataagacc aaaaccagca ataatcatta aaataattgt
     2581 tacaacttta attaaagcaa accaaaattc aaattctcca aacgctttta ccgagaataa
     2641 attcgctgcc attaataata aaatagcaat aacaccagga atccaagttg gcaattccgg
     2701 gaaccaaaac ttcatatatt ctcctactgc gatgacctca ctcataccaa ctacaatcca
     2761 ttggaatata ttactccatg ctgtcatata acctgctgca ggatgtatat aatcacttgc
     2821 aaatgttgca aatgatcctg ttgtagggtt taaataaatc atttccccca ttgctctcat
     2881 gattaaaaat aaaaagatac ccgcaattaa atatgcaagg ataactgatg ggcctgtcca
     2941 tttaattgta cttgtcgcac ccatgaaaag ccccacgcca atcgtaccac caagtgcaat
     3001 catttgaatt tgacgggcac tcaatcccct ttgtagttca ttgttctcca taacttctta
     3061 ccccatctct gattgatact aaataaatcc ataaaaaaac ttattgccgt aattatacta
     3121 taaattatcg attttggtac attaatattt gcataccatg caatcgttta cagaactact
     3181 atagcaggct tttgcaactt tgcaaacaat tattttgtga tttttctaaa aaattaaaaa
     3241 agttgctcaa gtaccgttga gtcacataca acttcagcta ttcttggtaa ttatgatata
     3301 attttaataa tattaaaaag gagtatatca atgttaaaac aaatactgct acgtgctaca
     3361 aaaatatcgg tactctttgc tgtagcattt ttcatcataa attatatcgg tatggaaaaa
     3421 cctgacatcc tatatttagt tggaagaact attattgcaa cacttgcttt catactcatt
     3481 tgtcttacag tatttactat tattaattct ccagagcgca agattaaact cggtacgact
     3541 ttaccgattg cattaattat tggtatcatt ttcggcgcca tatttttaac ggtacaaatc
     3601 ggtgtcatca ctgggttaat catcggtgtg attgctacgt ttatttggga attgattgaa
     3661 aaaaataaag gaggtcgttc atcatgacaa cagcattaat tggtgtaatt gtattaatct
     3721 tactaattat tagctttgtg ccaaactacc gtgctatgaa atcagcaaaa gaacaaggaa
     3781 gtaatgcaac aagagctaca attatggtag gcatagatat tatcttaatt gtacttatca
     3841 tcgtgacgtt attattaaaa tatatgtaac tatttaaacc accctatctc atacataaac
     3901 atgaacgttc taccatttca catacatatg gtcgtccatt taatgaggta aggtggtttt
     3961 ctcgttaaag tttaccttgt aatagtaaaa aatagccaat tgttgcgagt attaagtaaa
     4021 tcattgaacc aacaacaaaa gatcttactt gcacgcgaaa tggtgtctgt ttgttaaata
     4081 taggaccaat caccaaattt ccaacgccta aaattactgg accagctaga aaaaacaata
     4141 taatgtaacc catcatgata cgcctcctat ttcgtaaaga aacgcgctac aatttgatcg
     4201 taatcaatat cttttgctac ataatctaca ttagaaaagt cctgtgcgct tgtcatttta
     4261 ttcgtattaa caataacatc taatccagct gcaatcgcgg ctgttgcacc atttacagaa
     4321 tcttcaatag ccaaacattg tgtcggcata taatttaatt gttgtactgc agataaatat
     4381 aattcagggt caggtttaac ttgttcaaca tcttctctac caacaactac ctctatataa
     4441 tcgtctagac ctaatgcttt aaaggtgggc attatttccg ctttcacact acttgtggca
     4501 attgccatag gaatgtgacg ttgctttaaa aatgccatca atgatttaat cgtatcaatc
     4561 atcggtaatg ttgtactagt aagatgatgt tcttcataaa gtttatcttt attttccgaa
     4621 ccaatcgctt taattaaatg gtcatgcaaa tctgtagctg ctcctccaat agaagaacga
     4681 taaaaatcaa tgcttatagg atcggcatta tgcatctcta aatgtttatt aataacatta
     4741 aataaatgtt gttccgtatc tattattgtt ccatcgaaat caaatataac tgctctatac
     4801 atatatttac tcctctattt tttctcttta tcaaagctta acatagtccg atgaccactt
     4861 cattctatta gtgcaattat aacttttttg accaatactt tgctaaaata ggccctgtta
     4921 tattatgaat gaaactaaac actgcgcctg gtactgctgc aattggatta aaatgcaatg
     4981 ctgctaatga cacagctaaa ccagagttct gcattccaac ttcaatactg actgcttttt
     5041 gatcttgtcg atctaatttt aaaagacgag ctaaccaata tccaatcgta taccctaata
     5101 cgttatgtaa aataactact aaaaatatta ataatcctgt agttaagatt tggtgcttac
     5161 tgccacctac aactattgct aaaattaatg aaatagcaac aactgatata attggcaaag
     5221 ctgtagaagc tttttcagca attttacgat taataatttg caaaacaata cctaaagcaa
     5281 ttggaattaa tacaacttga acaactgacc acaacatact cacgaaagat acttccaacc
     5341 attcatttgc aaatagatat attaacgcag gtgtaacgaa tggcgctagc aacgtagaga
     5401 ccgtcgtaat agaaacagaa agtgctacgt tagctttggc taaataactc attacattac
     5461 ttgatgtccc acccggacaa catccgacta atattacgcc aacagcaata tcaggtggta
     5521 aatgaaaaga ctttgcaatt ataaatgcta atgtgggcat aatactgaat tgtagacaga
     5581 caccaataat tactgctcta ggtgctttaa agaccatttt gaaatcatta ggtgtaattg
     5641 ttaatcccat acctaacata acaataccaa gtaaataagg tacccattta cctaatgtcg
     5701 caacatgttg tgggaaaata aatccaataa cagctgctac taacatccat accaaaaaac
     5761 tcttcgtggc aaatatactc aatttttgaa acattgttaa cacatcctcg tcgataatat
     5821 tgaaaaatat taccataaga ccagtaaggt tacaattact tttctgaaaa ttaagattat
     5881 ttagtcattc tcttcttatt gcaaatattt tatcactgat aaacaattat tttaaacttc
     5941 attcaaaagt cttagtgaat aaagaaaaaa agcgtggtaa aatcaacttg aacaaaatta
     6001 agtcaccttt ataaatatta aacgtgtatt taatgccatg tattcctcgg gaggtcctca
     6061 catgttaatt gataaattcg aaacatacat catcaacatc gccggtttaa atgaccgtac
     6121 tactagaaaa aaattaagta agttatgcaa aagtgtacaa ttttgtgatg cgttgcaatt
     6181 ctcaataaac aaacagttta atcagtatgt attagaaatt agcttaccaa agcaacaact
     6241 tccatacttc atttcatttt taagttttca tcagtatagt attttccaag tactttcacc
     6301 aaagaaaata aatgaattac ttgattctga taatttatat caatctgcaa agcgtttcga
     6361 tatcaatatt gatggcttgc aagatgcttt cattaaagac aaagtcatcg atattatgaa
     6421 tatgttccaa aatcacactg atatcactta tacgttgaat aagtctcatg cacatattat
     6481 atgtacgcca gaaatatttg ctaaattgtt acatacgatt gcaactagaa atatcgacat
     6541 tctatctgcc aattatagat cgtcatctat gagcaaagct cgtatatcat aataaaagac
     6601 acatctatac gatgatcatt tacaatgatt gcgtatagtg atgtgtcttt ttttgtatta
     6661 tttatcttcg gacgattgat cctcaaccat ttgttcaaat gcttcacgaa cttgtggtac
     6721 tgtcatacct actacaactt ggatactctt accactcttt actaatccgt gtgccatttg
     6781 ttgatgcgtg aaatattccg tatctgcaac tttactttca tcataaacag ttaagcgtaa
     6841 tcttgttgta cagttagtaa catctttgat attttctttg ccgcctaaac cgtcgaggta
     6901 atatgcagct ttgtcttcgt attcattacc agatgaagca cgtttagaat ctacgctatc
     6961 acctttttta tttttgtaat cttgcttaga aaataattta acttcctctt ctgttttctt
     7021 acgtccaggt aatggaatat caaatttcaa aattaagaat ctgaataaga agaagtaaat
     7081 cgctacaaat atcagaccga taataacttg tgcaacataa gtaccccaat gttctttacc
     7141 taatggcaac cagtttgttg aaataaagtc taacaagcca ccacccatgt tacctacaac
     7201 accaaatgcg tacattaaag tatccattga tgctgctaaa actgcgtgta aaacaaataa
     7261 gtatggtgcg atgaataaga atgtaaactc taatggctca gtaataccta caacgactgc
     7321 tgttaatgtg gcaggaataa gtagtgccgc caccttttta cgattttctt taggtgtcgt
     7381 agcatacatt gctaaagcaa tacctaaaca tccaaatact ttcccatttc cttgaagcat
     7441 aaagccataa gggaattgct cttttaatgg cttagtactc ttagcaaact catttaaatg
     7501 ttgtaaccat tcagctttca agccgtgatt gactactact ggtccaactt caattggtgc
     7561 ataaataaag tgatgtaatc ctgttggtat taatacacgt tctaagaagt gatacaacca
     7621 tacaccgata tagcctgaag caatgataaa gtgttgcatt gaaccaatac cgtgctgaac
     7681 agttggccaa ataacacaag taattgctgc taatggtaac atcacaaaaa atgaaatcgt
     7741 aactacaaat gttaaacctt ggaatacacc aaccatttct ggtaatcgtt tactatagta
     7801 tctattgtga atccaagtaa taatagctga aataataata ccacctagaa tattcgtatc
     7861 caatgtggca atacctgcaa ttgattttaa accaggtaca ttttcaacgc ctttttctaa
     7921 attagcgcca aacgtatgtg gccattgtgt taaaatggca tttataaatg tattaaacat
     7981 taagtaaccc atcaatgctg caagtgctgc atgacctggt gcttttttag ctaaagaaag
     8041 tggtagacct acaacaaata caacttccat atgcgtaaaa ataacccagc caccagattc
     8101 tattactgac caaaatttaa accaaaacgt atgttgatct gctaaactcc ccatgattgt
     8161 aggattttta aataatgtcg caaagccaag cactatccca aagaaagcga acattaatac
     8221 cggtacaatc attgcactac cgaaacgctt tatcgcattc atcttctatt ccctccatat
     8281 catctttcct aacaatacat ctaattagat tcatttataa atagatgtct tactatttaa
     8341 atataatata tagtaaacgc ttacacacct acaacgacat tgacgtattt tgaaagtatt
     8401 ttgtataatc agattatctt ttcatatagt gaaaattttt tcacgacctt atatatgaca
     8461 tcgttgtatt tgtaatacat tcgttttaaa cgcataatca aacctatatc aatacacaaa
     8521 tatatataat gacatacaag attttaatgt aataacgatc tattacacat ttattttcaa
     8581 ggaggttgaa tatgttttta gatgaacaca ttaatcgaaa ctttgataaa cttaatgata
     8641 atgatttaca tatcgctcac tttatcaata cacatataga tgaatgtaaa aatatgaaaa
     8701 tacaagattt agcgcaattc acacatgcct cgaatgcaac cattcacaga tttacacgca
     8761 aattaggttt tgacggttat agtgatttta aatcgtacct taaatttgaa agtaataaga
     8821 cacaccaatt accttctaac tctatcgata gtttcaaaca agaaatcgat agtacattca
     8881 gttatttaga acgcgttgat taccagcttc ttactaaaaa aatgcataca gcttcaacaa
     8941 tatatttgta tggtacaggg cgtgcacagt taaacgttgc ttcagaggca cagcgtatat
     9001 tgttaacgtt acataatcat gtcattgtac ttcatgatga acatgaactt aaaatgattt
     9061 tgaacaagag tgaacctaat gatttattct ttattatttc attatcaggt gaaacacatg
     9121 aattattaga agtaacaaac ttgcttcaac ttagacaaaa atactttatt tctgttacta
     9181 caatgaaaga taataccctt gctcaaaaag caaattacaa cgtctatgta tccagtaaca
     9241 cattctactt aactgatggt acagactatt ctagcttcat cagttatcat atattctttg
     9301 aaacacttgt aagaaaatat aatgagtatt tactacataa tgatatttca aattagaaaa
     9361 aaggaagtta tgatttaatg gccttgagcc tatcataact tccttttatc attttattgt
     9421 tgtgttgatg tttcgataac gtggtacatc ttatcaaaca tcaattcgaa accatgcacc
     9481 atggcatcat gatattcttt tttcttttgc ttgtattcta aattagtaaa tcgtctttct
     9541 ttttcaacta atgaacgata ataaaatagc atttgggtgc cacctgtttc acgttcaaaa
     9601 aattctacct caatgacatc ttgcgtttca cttagtccag gcataccgat agtcatctta
     9661 acgtattcat ccataactaa agattcataa atgccttcaa tcacatttac tttgccatta
     9721 cgttgttgat ctacaatacg atatttaccg ccttctttaa cgtccgcttc aatctcttta
     9781 ttcgttctgg ctgatgtcat aaaccattgt ttcaacaaat ctttctttgt ccaagcttcg
     9841 tatactaact ctggagaaaa tttataaagc ttttcaattt caacttcgac atgttcattc
     9901 tctacattaa attttgccac tgttgtccac ccactttcgc tcttactttt attttaacgt
     9961 atttttgctc agttccaaac atagatgatc atcattttta aaagattagc gttatacggt
    10021 gagtacaaca tgatctgtta atataacaag ccaccttact tggctacatc gatatattgt
    10081 taagcattaa tgtttcattt cttgactagt gttctttttt agctttggaa aattaaataa
    10141 aatcgcaata agtccgcata cacctaataa tataggataa atgctgtatg ggaataacat
    10201 taacggtgaa ataccagcta caccagccgc tgaaatgact tgcgggctat atggtaataa
    10261 accttggaag cagcctccaa atatatcaag aatacttgct gatttccttg aatctacatc
    10321 atattcatct gcaatatttt tagctaaagg acctgacata ataatagaga tggtgttgtt
    10381 tgccgtggca atatctgcga cacttaccaa actagcaatt cctaattctg cgccacgctt
    10441 tgatttcact ttagagcgaa caaattgcaa caaccattca ataccaccat tgtgttgaat
    10501 aataccgact aaaccaccaa ttagcaacgc aatcatagca atatcttcca tgcttataat
    10561 acctttggac actgcatcta gtagccccat ccaaccgaat gaaccatcta tgagaccaat
    10621 gattccggct aataatgttc cgccaatcaa tacgataatg acatttacac ctaataatgc
    10681 taataccaat actaagatat acggtacaac tttaattaga ttataatcat agtttttagc
    10741 atgatttaaa gaaatgccat tcgttaagaa atacagaata ataatcgtta aaatagcacc
    10801 tggcaataca attttaaagt ttactctgaa tttatctttc attttcgtat gttgtgttct
    10861 aaccgcagca attgttgtat ctgaaatcat tgatagatta tcgccgaaca ttgcacctcc
    10921 aacaactgta gccattgcta gcgcagttgg tacatctgtc gcttgtgcaa atccgtaacc
    10981 aacaggtgct attgcagcaa cagttcctac agacgtcccc atagatatag aaacaaacat
    11041 acaaatcaca aacaatccta caataattaa attttctggg attaatgata gtcctaaatt
    11101 aactgtcgac tttacgccac ccattttttc agctgtattt gaaaatgcac ctgctaaaat
    11161 aaaaatcaac atcattaaaa caatgtttga atggcctgca cctttcgtga agacctcaac
    11221 ttttttagca aatgattctt ttcgattcat taataacgcc acaattaccg ttatcgtaat
    11281 tgcaacattt aatggcattg aagtaaaatc acctgtgata atacctacgc ctaaaaacaa
    11341 cgccacaaat aataacaagg ggaataatgc ccaagcattg ctctttttat gtacttccat
    11401 cctttttacc tgctttccaa ttaaaaatac ctctttctca caaacgatga agaaagaggt
    11461 tttcatgtgc tttacctgct tatcttcaaa ccattacggt tactggaatt ggcacattcg
    11521 agatgttgcc gaggcttcat agggccagtc cctccacctc tctagataag tgatgcttat
    11581 ttacgtttac gttacaagat aatccttagt acgtcaatca taaattaatc aggagtcgta
    11641 taatattttt cataaacaat cattgctact gtaataataa tcaaaacaat aatgctaata
    11701 acaagtaaaa gccaccattt aagcattaat gcaataaaaa tgaacacgat agacacactt
    11761 actaatatta atgatatgac tttaaattgc tgaacacgtt gcttggagat gactttcaac
    11821 tgtttgtttg atagacgcgt attttttata ctgattccca gtatattttc taatatttga
    11881 accaatacga tacttattgc aaatataata attggtaaaa catcatagct ccctatagtt
    11941 aatgtataaa ttacaaatcc aatgtaaagt aaccctgaga caaaggataa aaagtatgcg
    12001 acgtatttgt taaacttaat gatatgcttt ttaacgtttt gatgtgtaaa ccatacattc
    12061 gaaacgatcg caactgctac aaataatgtg aatactatat ataatggtaa tttttgttca
    12121 ggaaaaacag tcgctattcc aaaagctaat gctaaaatca aaaataatat agctctagat
    12181 actattaatg ccataataac aacccctttg tttaatatcg agtttgcaaa tttacgttta
    12241 tcagcgtttc tatgatcagt acttctacgg gtagcgtttc tatgtaattt acatcatctt
    12301 aacatataaa tacttcgcta tttaattgaa aacatatcct attattcttt gtccgttctg
    12361 acgtttaata tctagcctta ggcatttcac ttgttaatga atttaacttt cttccactaa
    12421 ccgtccctaa acccaatccc gcaacagttt ttaacttttt cgttgttgtc ctgacatcct
    12481 cattaagaaa gtttattctg cttaaaactt ataatccaca ccctgagcaa acgctcctta
    12541 tgacagagta ttaaaataag ccgataaaga tacacacctt taccgactat ttaaaataca
    12601 cttcaccaat tcattttaat ttaatggatt gaagtaacta aattaatatt atgttgttca
    12661 attaaaagct tcatacaaac ctaatctatt tgcactccac cgctaacacc gaacacttgt
    12721 ccggttgtat aacttgattc ttctgacgct aatagcacat aagttccaca taactcaaca
    12781 ggttgacctg cacgacctaa aggtgttttt tgaccaaatg ttgggatttt actttgaggt
    12841 tgtccaccag aaatttgtaa tggtgaccag aatggaccag gcgctacaca gttcactcta
    12901 attccttttg gtcctaattc ttctgaaaaa cttttagtta atgaaataat tgctgctttt
    12961 gaagcggcat aatcatgaag aataggacta ggattataac cttgtacaga tgatgtcgtt
    13021 gtaattgacg cacccggttt taaatattcc aatgcttttt gaactgtcca aaatagcgga
    13081 tagacattcg tttcaaatgt ttctgtaaat gcctcagttg taaatccatg aatatcatca
    13141 tgatactgtt gatgtccagc aactaaagta acattatcta agccacctaa ttgttgatat
    13201 gcttgttcaa caaggtcata gttgaactgt tcatctctta tatcaccagg aattaacact
    13261 gccttttgac cactttcttc aatcacttgg cgtacttctt gtgcatcttg ttcttcactc
    13321 ggaagatagt taatcgctac atctgcacct tctttagcat acgcaattgc tgctgcacgc
    13381 cctattgctg agtcaccacc tgtgactaat attttatagc cttgtaagcg ttgatgacct
    13441 tggtaagacg tttcgccaca atcgggtgct ggcgtcattt cagattgtaa acccggtacc
    13501 tcttgttctt gtttttcata atccgttgtt ttaaattttg ttctaggatc ttgagctgcc
    13561 atttttttac atctccttat tcgcttaatg gttattattt acccaatctt cctaggaact
    13621 taatcatgat tacactaaaa attactttct tctttataaa aacaagctcg aattattcat
    13681 gcaatagtct ctttacaaat tcaacaaaat actcaggtac tttttccaga atcctttcat
    13741 ccggtttata ttgaggatga tgtaaatcat attcactatg agaaccaatt aacgcaaata
    13801 cacttggaaa atgttgacta taacctgaaa aatcttctcc aatcgtaagc ggctgttcca
    13861 tcatttccac cttatatcca acatgttggg ctactgcaat tgctttatgc gtcaatgcct
    13921 catcattcat cacagcgcca ggtaaatgcg tataatttaa attgattttc atattatatg
    13981 cttgagccaa tccgtccgca atatcttgta atcgtgtttc tacaagcttt cgtaccacag
    14041 gatcaaaact acgcactgtg ccttgtacat acgcatgatc agcaatgaca ttccaagtat
    14101 taccacatga tatttgtcca attgttacta ccgcttcatc aaacgcagat agatttctac
    14161 taactatgga ttgaatacta ttaatcaatt gcgccaacac aataactgga tcgttgcatt
    14221 gttctggctt tgcagcatga ccacccacgc ctttaatatg aaactcaaaa cgatctactg
    14281 ctgatgtaat tgcccctgtt ttgattgcaa atgtacctac cgaacgcgat gggtcattat
    14341 gaaaacccaa tactgcttgt acatctttta atgcatgtgt ttcaataatt ttaaaagcgc
    14401 catgtcctag ttcttctgct gattgaaaaa tgaatttaac acgcccagta agagtgccct
    14461 caatttcttt taattttaca gctgtagcca aaatactagc catgtgaata tcatgaccac
    14521 acgcatgcat aacaccttca ttttcagatt taaaatcctg ttcaacaagc tcttgaatcg
    14581 gtaaagcatc aatgtcagcc cgaacagcta tacaactcag accttgtccc acttcagcaa
    14641 caagcccagt cgcaagtggt aagtctaata ttctaatatg atgttctgtt aaaatatctt
    14701 taattttttg tgtagtctta aattctttat cggatagttc tggaaattga tgaaaatacc
    14761 ttctccaggt aacagcttga tcttttaatc ccatcggtca ttccccttcc ttaagtcaat
    14821 gatatgttgt ctaccctacg atgatcatct ttgactatta aacgatgatt tcacaacaat
    14881 gtactcttgt taattgcttt cgttaatgat agacagttgt ttaataatat cgtaacactg
    14941 ttgtcaaact attctaactt ttataattga gactctatac aaaaacgtgt tctcgaatat
    15001 acttgttttt acaaaccaca aaaagctcta aacattagtt taaaccaatg cttagagctt
    15061 tctaattatt ttatgcttta aaagatactg tgttatctac gatgacctta ccgtctttaa
    15121 taactttttc tgcgtgattg ataccaaaat gatatggaat atattcatga tttggtgcat
    15181 cccaaattac taaattagcc ttatcacctg tgttaattgt acccgcgtta atgtctattg
    15241 ctttagcagc attgaccgta acagcattcc aaacttcatt aggtgatagc tttaatttca
    15301 aggctgcaat cgccataaca agttgtaagt tgtttgtgac actactacca gggttataat
    15361 cagttgctaa tgcaatcgca ccgttattgt caagcatgcc tcttgcatct gcataatctt
    15421 ctttacctaa atagaacgtc gttgcaggta agaggacagc tacagtatca ctatttcgca
    15481 acttttcttt tcctttatca ctagaagcta ctaagtggtc tgctgatatt gcttgttcat
    15541 caattgctaa ttccagtccg cctaacggat caatttcatc cgcatgtatt ttcactttaa
    15601 aacctgcttc tttggctttt tgcatataat gttgcgattg ttctattgta aatacacctg
    15661 tttcacagaa aatatccgca aagtctgcat attgttttac ttccggaagt aacgcaatca
    15721 tttcttctaa aaatgcctca tttgaacttg cctctttagg tacagcatga ggccctagga
    15781 aagtatgttt catgtctaaa tcatatttct cagctaaacg attagacact ttcaattgct
    15841 tcagttcatt ttctctatct aatccataac cactcttact ttcaactgca agcacgccgt
    15901 gtttaatcat agtaagcaaa tcatgctctg cttttttaaa caagtcatct tcggatgttt
    15961 ctctagtagc attaacggta gataatatgc caccacccat ttctaatatt tcaaggtaag
    16021 acttaccttg acgttttaat gacatctcat gttctcgaga tccaccaaat gttaaatggg
    16081 tatgtgcatc tactaatgct ggggacacta ccttcccact agcatcaatc gtctcagtcg
    16141 catcgtagtc atctgtatgt gttccagcat atacaatttt gccatcttta atgacaactg
    16201 taccattttt cacaacattt aattcatcta attccttacc cttcaaaggt ttatctgttg
    16261 atctcggtaa aattaattct gctatatgat taattattaa atcattcatt acttatcacc
    16321 tgctttatca atcattggaa tatgaatacc tttttcttta gctgtttgaa tagcgatgtc
    16381 atagccagca tcaacatgtc gggcaacacc catacctggg tcagtcgtca atacacgttc
    16441 caatcttctt tcagcacgct ctgatccatc tgctacaaca accatacccg catgaagtga
    16501 atatcccatg ccaacaccgc caccgtgatg gaatgaaatc catgaaccac ctgcagctgt
    16561 gttaatgagt gcattcaata cagcccaatc accaaccgcg tcactaccat ctttcatact
    16621 ttctgtttca cggttaggac tagcaactga accagcatct aaatggtctc gtccaataac
    16681 aattggtgct gaaatttcac cgtcacgtac aagacgattt aaagctaagc ccattttcgc
    16741 tctttctcca tagcctaacc aagcaatacg tgatggtagt ccttgatatg aaattttttc
    16801 ttcagctaaa tcaagccatc ttaataactt ttcattttct gggaaaagtt tgcgcatttc
    16861 ttcatccgca cgctcgatat cttttggatc accactcaac gcagcaaagc ggaatggccc
    16921 tttaccttca cagaataatg gtctaatgta agctggtaca aagcctggga agtcaaaagc
    16981 atttttcact ccgttattga aggctacttg acgaatattg ttaccataat caaatgctac
    17041 agcgccacgt ttttggaatt caagcattaa ttcaacatgc tttgccattg aagcttgtga
    17101 cagttcaaca tattttttcg gatctttttc acgcaatact ttcgcttctt ctacagagta
    17161 tccttgtggc acatatccat ttagcggatc atgtgcactt gtttggtcag taataatgtc
    17221 aattttaaat cctttttcta gaatcgcttg atggatgtct acagcatttc caactaaccc
    17281 gattgataat ccttctccac gttctttcgc ctcttctgct aattttaatg cttcatctaa
    17341 atcagctgtt ttaacatcac agtatttcgt atcaattcgc ttatcaacac gtgtttcatc
    17401 aacatccacg caaattgcta ccccatgatt catagtaatt gctaacggtt gcgcaccacc
    17461 cataccacct aaacctgctg tcagtgtaac agtgcctgct aaatctccat taaagtgttg
    17521 attacctagc tcggcaaatg tctcataagt accttgcaca ataccttgag aaccaatata
    17581 tatccaacta ccggctgtca tctgtccata catgattaaa ccttttttat ctaattcatt
    17641 aaaatgatcc cagtttgccc attcaggcac taatactgaa tttgaaatta atacacgtgg
    17701 cgcttcttca tgtgttttaa atacagcaac tggctttcct gattgtacta acattgtctc
    17761 atctgattct aattctcgta acgttttctc tattgcttca aaagcttccc aattacgtgc
    17821 tgcttttcca ataccaccat aaacaactaa atcttctggt ctttcagcaa cttctgggtc
    17881 taaattgttg tataacattc taagtactgc ttcttgttcc caacctttac actcaatact
    17941 caaacctttt tttgcttgaa tttttctcat aaaattcgct cctgttcttt taagaagtta
    18001 attccactaa atttaaaacg cttacattat tatcttcaat attcattata gtatgttaaa
    18061 atatagccaa caaatataaa taaactaatt atccatagct tgaatctata aataaaagga
    18121 gcaaaacaca tgaaaattat tcagttagaa tacttcttgg ctatcgtgaa atataatagt
    18181 tttactaaag ctgcacaatt tttacatatt agccagccat ctttaactgc tacgattaaa
    18241 aaaatggaag cagatttagg ttatgactta tttacacgtt caacaaaaga catcaagatt
    18301 accgaaaaag gaatacagtt ttatcgttat gcgagcgaat tagttcaaca atatcgatcc
    18361 acgatggaaa aaatgtatga tttaagcgtt acatcagaac caaggataaa aattgggact
    18421 cttgaatcta cgaatcaatg gattgcgaat ttaattcgaa agcaccattc cgactaccct
    18481 gaacagcaat atcgtttata tgaaatacat gataaacatc aatctataga gcaattactg
    18541 aattttaata ttcatttagc tataacaaat gaaaaaataa cccacgaaga tataagatcc
    18601 attcctttat atgaggaatc ttacatttta ttagcaccca aggaaacatt taaaaatcaa
    18661 aattgggtag atgttgaaaa tttgccactc atattaccaa acaaaaattc tcaagtgcgc
    18721 aaacacttag atgactattt taatagaaga aatattcgtc caaatgtcgt tgtagaaaca
    18781 gatcgattcg aatcagcagt tggatttgtt catctcggct taggttacgc tatcattccg
    18841 agattttatt accaatcatt tcacacgtct aatttagaat ataaaaaaat tcgtccaaac
    18901 ttaggccgaa aaatttatat caattaccat aaaaaacgca aacactccga acaagtacat
    18961 acattcgtac aacaatgcca agattattta tatggacttt tagaggctct ttaacttaag
    19021 ttattagagc ctcttatgca gttgcgcaga tcatcgtata aaaattaatg acgtcatttc
    19081 aaaaatcgat acaaaaataa tttattataa aaattctaag aaagaagtga agcagatgtt
    19141 aaaatctatt aatcatatat gcttttcagt cagaaattta aacgattcaa tacattttta
    19201 tagagatatt ttacttggga aattgctatt gactggtaaa aaaactgctt attttgagct
    19261 tgcaggccta tggattgctt taaatgaaga aaaagatata ccacgtaatg aaattcactt
    19321 ttcatataca catatagctt tcactataga tgacagcgaa tttaaatatt ggcatcagag
    19381 gttaaaagat aataacgtga atattttaga aggaagagtt agagatatta gagatagaca
    19441 atcaatttac tttaccgacc ctgatggtca taagctagaa ttacatactg gcacacttga
    19501 gaacagatta aattattata aagaggctaa accacatatg acattttaca aataaggtgt
    19561 cattataaaa aggcctcttg aactccgtta aaattttaat taattattat ataataagag
    19621 aacttttcaa acaata
//
LOCUS       DALAGD010000265         6869 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.guided.123, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000265 DALAGD010000000
VERSION     DALAGD010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6869
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.guided.123"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..83
                     /locus_tag="O2701_002900"
     CDS             <1..83
                     /locus_tag="O2701_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCZ4294726.1"
                     /translation="FVHIIAPVISGCAVAYYTYWLSKRNK"
     gene            complement(255..1226)
                     /locus_tag="O2701_002901"
     CDS             complement(255..1226)
                     /locus_tag="O2701_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCZ4294727.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKD
                     MLALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEY
                     AQVPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGG
                     VGSVAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLD
                     TVGSNVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIR
                     EDIWSKLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
     gene            1350..1769
                     /locus_tag="O2701_002902"
     CDS             1350..1769
                     /locus_tag="O2701_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCZ4294728.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLIN
                     SNLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWP
                     TLEKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
     gene            complement(2295..3140)
                     /gene="blaZ"
                     /locus_tag="O2701_002903"
     CDS             complement(2295..3140)
                     /gene="blaZ"
                     /locus_tag="O2701_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="HCZ4294729.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALN
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMFNNKNGDTLIKDGVPK
                     DYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
     gene            3247..5004
                     /gene="blaR1"
                     /locus_tag="O2701_002904"
     CDS             3247..5004
                     /gene="blaR1"
                     /locus_tag="O2701_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HCZ4294730.1"
                     /translation="MAKLLIMSVVSFCFIFLLLVFFRYILKRYFNYSLNYKVWYLTML
                     AGLIPFIPIKFSFIKFNNVNNQAPTVESKSHDLNHNINTTKPIQEFTTDIHKFNWDSI
                     DNICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIV
                     IRKAEAIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFN
                     IFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLKTPFSN
                     KNILSHSFNGKKSLLKSRLINIKEADLKKQSKLILIFICIFTFFIMIIQSQFLMGQSL
                     TDYNYKKPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTYK
                     IYLALFGLDRHIISDKNSRMSWNHKHYLFESWNKEQDLNTAMQNSVNWYFERISNQIP
                     KNYTAAQLKQLNYGNENLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQ
                     LSSSLLIKKNEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGNPSGK
                     NAELISEKILKGMGVLNDQ"
     gene            4994..5374
                     /gene="blaI"
                     /locus_tag="O2701_002905"
     CDS             4994..5374
                     /gene="blaI"
                     /locus_tag="O2701_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HCZ4294731.1"
                     /translation="MTNKQVEISMAEWDVMNIIWDKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
     gene            5638..6216
                     /locus_tag="O2701_002906"
     CDS             5638..6216
                     /locus_tag="O2701_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCZ4294732.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPG
                     LDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNG
                     TPIKTIAEQWQVSRTTIYRYLNKLEAKENKNK"
     gene            complement(6439..6624)
                     /locus_tag="O2701_002907"
     CDS             complement(6439..6624)
                     /locus_tag="O2701_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294733.1"
                     /translation="MNKLEKWSLVFIIFTFLSSLALLYFKYNFSSVMPVLSLSILTIL
                     ILLTSIITVVSVNDEDT"
BASE COUNT         2574 a          950 c          945 g         2400 t
ORIGIN      
        1 ttttcgttca catcatagca ccagtcatca gtggctgtgc tgttgcgtat tatacttatt
       61 ggcttagtaa acgcaacaaa taattaatag gcgactttat aagccagcat cgaaaaaatc
      121 cccacactac tgcaatagtg tggggattgg tgcattaatt gttaatcatt ttcgttatat
      181 aaattataac actggttagt agagaaatgc aaaatgattg aattaatagt aaatgtatta
      241 atgaatactc acacttatac ttttaaaata gttcttccta aatgttgtcc ttttaaaagc
      301 tgatctatcg ttttatagaa ttcttcaaat ccaatttcat tgtatagaag tgaatttcct
      361 atattccatt catttgctaa cttactccat atatcttcac ggatattatg atcaacatat
      421 acagaatcaa tgccaattaa tttaatacct ctaagaataa acggtaatat attagcgtta
      481 agtgaatttc ctgctacatt tccacataca gtagcaacac cttgataatt tagacgtttt
      541 aaaccgtata atgttacatt actccccact gtatctaaga aataatcaaa aatacctttt
      601 ttaagcggtt tattatcatc ttcaattatt attacttgtt ttgcacctaa tttttttgcg
      661 atttctattt gcttttcttt tcttacgata gcagtgatat tttcatatcc aattttatgt
      721 aaaatttgta tagcaacact gcccacacca cctgtagccc cagacactaa aatttcaggt
      781 tgtgatttga tattcattcc atatttttct agttcaaaaa ttgataatgc tgccgtaaaa
      841 ccggctgtgc catataccat agactcttta atagacattt cttggggaat aggaacaatc
      901 cactctccag gcacttgtgc atattctgaa aatccacctg tatgtgtcac gccaacatca
      961 taaccagtaa ctaatacatt atcgccttct ttaaacttat cattcgaact tgattcaaca
     1021 atacctgcaa aatctatacc aggaatcata ggataatctc taattacacc gcctttatat
     1081 tgtaaagcta gcatatcctt ataatttatt gaagagtata ccacttttac taatacatca
     1141 cccttcgata aatgagattc atcaactttt tctactttat aactaacatt tgatgcactt
     1201 tttctcacta ctaccgcatt gaacatttat tagccttctt tcacaattgt tttgtagaag
     1261 tatgtttttg ttgcaaaact attttcctac aaaacttatc atgatatgat aagcgagtca
     1321 atactaatga tttaagagag gttttagttg tggaattaga taattgtata aattatttgt
     1381 tgagtacttc tcagaataga gtttttaaac attttaaaag tttattaagt ccctataata
     1441 ttactccagc agaatatggt gttttgaatt gcttgattaa tagtaatttg aatacaccta
     1501 aagcgattgg taatacttta aatttagaac cttcaacaat atctggaata ttagataaga
     1561 tgtccaaaaa ggatctaata acacgtcaaa ctgataatga aaatagaaga actgtgctaa
     1621 taaacagtac aaataaagca aaagacttat ggccaacatt agaaaagaaa gcctatcaac
     1681 ttaataatca ttacttcgct aatttaacta atgaagaaac tgtagtgttt aaaaagatat
     1741 tactaaagat aaataaaact acgttttaat ttttacgtta taaaaaagtt gaagtgcgaa
     1801 ttataatcga gtaaatataa atgatagctt agcttaatta tatattaaag ccaactatac
     1861 ttttattccc gaaaaaccta ctatccaaat ttctaagata ctttagcctc tataataaac
     1921 atattgtaat ataatttatg tattatttag gtttacccta atcgaacaat aaaaaatact
     1981 tgtaaaatgc tattgtcact ttgcttgata tatgagaatt atttaacctt ataaatgaga
     2041 aaaaagcaac gcactttaaa taagatacgt tgctttttcg attgatgaac acctataatt
     2101 aaactattca tctattattt atgatttttt gtatatacaa tatttctagt ttgttaaaga
     2161 gaattaagaa aataaatctc gaaaataata aagggaaaat cagtttttga tatcaaaatt
     2221 atacatgtca acgataatac aaaatataat acaaactata agatgttatc agtatttatt
     2281 atgcatttag aatattaaaa ttccttcatt acactcttgg cggtttcact tatcaactta
     2341 tcatttggct tatcactttt attgtcttta ttcgtaaaaa tgactaaaac aataggttca
     2401 gattggccct taggataaac aaaagcaaca tcatttctag aagcatatgt tattgcttga
     2461 ccacttttat cagcaacctt atagtctttt ggaacaccat ctttaattaa agtgtctccg
     2521 tttttattat taaacattaa atcaagtaag aaatttttat tttttttgct taattttcca
     2581 tttgcgataa gtttatttaa agtcttgccg aaagcagcag gcgttgaagt atcttttttg
     2641 ctctttggtg aatagtaatt taattctatc tcatatctaa ctggatttgt tactttatct
     2701 cctagttctt ttagacgttg tttaactttt ttgattccac cgatttcttt tataatttta
     2761 ttgtttgctg tattatcact atatgtcatt gaagcctcaa taagttcttt taaagtgata
     2821 tcttttccta catatttttc taaaatagga gaataagcaa ctatatcatc tttgttaata
     2881 tgtacttttt tatttaactt attataaggt acttgttcta acaaaatagc actatttatc
     2941 gcttttgaag tcgaagcata ggcaaatctc ttatctgaat taaattttac ttccttacca
     3001 cttttagtat ttaaagcata aacaccaata tgagcattat attttttttc taaatcattt
     3061 aactctttgg catgtgaact gtttgaatta catgcactta aaactaaagc aattacaatt
     3121 aaaaatatta actttttcaa aataaaccct ccgatattac agttgtaatt tttataaatc
     3181 aataatatta caattgtaat attgatgtca attgtttatt ataaatattt gaaagaaggt
     3241 gtcaaaatgg ctaaattact aataatgagc gtagtaagct tttgttttat atttctattg
     3301 ttagtatttt ttaggtacat attaaaacgc tattttaatt atagtttaaa ttataaagtt
     3361 tggtatctaa ctatgcttgc tggattaatt ccttttatcc caattaaatt ctcttttata
     3421 aaatttaata atgtgaataa tcaagcgccc acagttgaaa gtaagtcaca cgatttgaac
     3481 cataacataa ataccaccaa acctattcaa gagttcacaa cagatatcca taagtttaat
     3541 tgggattcaa ttgataatat ctgcacagtt atttggatag ttttagttat tattttaagt
     3601 tttaaatttt tgaaagcctt attatatctt aaatatttaa agaaacagtc actttatcta
     3661 aacgaaaatg aaaaaaataa aatagatacg atacttttca accatcaata taaaaaaaat
     3721 attgtgattc gaaaagcaga ggctattcaa tctccaataa ctttttggta tgggaaatat
     3781 attattttga ttcctagttc atattttaaa agtgtaattg ataaaagact aaaatatata
     3841 atactgcatg aatatgctca tgctaaaaat agagatactt tacatttaat tatctttaat
     3901 atcttctcta ttattatgag ttacaatcca ttagtacata ttgtaaaaag aaagataatt
     3961 catgacaatg aagtagaagc cgatagattt gtccttaata atattaacaa aaatgaattt
     4021 aaaacttatg cggaatctat tatggactcc gtattaaaaa ccccattttc caacaaaaat
     4081 atattaagcc attcatttaa tggtaaaaaa tcattactca aaagtagatt aattaatata
     4141 aaagaagccg atctgaaaaa gcagtcaaaa ttaattctaa tttttatttg tatttttact
     4201 ttttttatca tgataattca aagccagttt ttaatggggc aatccttaac tgattataat
     4261 tataaaaagc ccttacaaag cgattaccaa attttagatg aaagtaaaaa ttttggttca
     4321 aatagtggat ctttcgttat gtacagcatg aagaaagaca aatactatat ttataatgaa
     4381 aaagaaagta gaaaaagata ttctcctgat tcaacttaca aaatttattt agcactgttt
     4441 ggacttgacc gtcatattat aagtgataaa aattcacgta tgtcatggaa tcataaacat
     4501 tatcttttcg agtcttggaa taaggaacaa gatttaaata cagcaatgca aaattcagtt
     4561 aattggtact tcgaacgtat tagtaatcaa ataccaaaga actatactgc ggctcaactc
     4621 aagcaattaa attatggtaa tgaaaatttg ggaagttata aaagctattg gatggaagat
     4681 agtttgaaaa tatctaatct tgaacaagta atagttttta aaaatatgat ggaacaaaat
     4741 aaccatttta gtaaaaaagc aaagaatcaa ttatcttctt cattattgat taagaaaaat
     4801 gaaaagtatg aactgtatgg gaaaacaggt acaggtatag taaacgggaa gtataataat
     4861 gggtggtttg taggttacgt aattacaaat catgataagt attattttgc tacacattta
     4921 tcagatggaa acccgtctgg gaaaaatgct gaattaataa gtgaaaaaat attaaaagga
     4981 atgggtgttt taaatgacca ataagcaagt tgaaatatct atggctgaat gggatgttat
     5041 gaatataata tgggataaaa aatcagtatc agctaatgaa attgtagttg aaattcaaaa
     5101 atataaagaa gttagtgata aaacgattag aacattaatc acaagactat ataaaaaaga
     5161 gattataaaa cgatacaaat cagaaaatat ttatttttac tcatcaaata ttaaagaaga
     5221 cgatattaaa atgaaaactg ctaaaacctt tcttaataaa ctgtatggag gggatatgaa
     5281 aagtttagtg ctgaattttg cgaaaaatga agaattaaat aacaaagaaa ttgaagaatt
     5341 gcgagacatt ttaaatgata ttagtaaaaa gtaattagag gtatgtttca aacataatac
     5401 atatgtaaac catacttgac tcttattaaa ttagttttgt taatgacatt taaaatatat
     5461 tattaaagat gagagtgtcc agaaaataac cattttctgt acactcttgt tttttgtcta
     5521 aataagtaag tattacatag aattatgtgt tcaaaaaccc aacaaaaaat ctatgttcac
     5581 aaaagacctc gtaatcaagt tatggtacac taaaggtgta tataggaggt ctgtattttg
     5641 aaaataggtt atgcaagagt ttcaactgga ttacaaaatt tgaatttaca ggaggatcga
     5701 ttaaatacat atggttgtga aaagatattt aatgaccata tgagtggttc aaaaagtaag
     5761 cgacctggtt tagataaagc aattgaattt gcgaggtcag gagatacgat tgttgtttgg
     5821 agattagata gactaggacg gaacatggag gatttaatca cgttagttaa tgaacttaac
     5881 gaacgaggtg tgagttttca tagtttagaa gaaaatataa cgatggataa atcaagttct
     5941 acaggacaat tattatttca tttattcgca gcatttgcag agtttgaacg taatttaatt
     6001 ttagagcgat catcagccgg tcgaattgca gcacgtgcta gaggccgata tggaggtaga
     6061 cctgaaaaat taaataaaca agatttaaac ttacttaaga cactttatga taatggtacg
     6121 ccaattaaaa caattgcaga gcagtggcaa gtttcacgta caactattta tcgttatctc
     6181 aataaattag aggcaaaaga gaataaaaac aagtagagca actaatctag cttactataa
     6241 tgaatctact aaataatttt atatatcatt aacatacaaa gactttgatt caatgtcact
     6301 cgatgtaaaa gagacgttct gacagcgtct aaaatctcat aatattttcc tgttatgaga
     6361 ttttaataat aacacaagaa aaaagcgtac aaattcgtac gcttttttaa tataatacct
     6421 gtttcactaa tgaacgtttt aagtatcttc atcattaact gaaactacag taataatact
     6481 cgtcaacaat attagtatag ttaaaataga aagacttagg actggcataa ccgaactaaa
     6541 attatatttg aaatataaaa gtgctaatga agagagaaat gtaaaaataa taaataccaa
     6601 agaccatttt tctaatttat tcattggctt cacctctttg aacttttata taatcatagg
     6661 agatattttt actacgattt ataaaaaata agaagataat cattattaaa gaaacgatta
     6721 atataatgtt gaatcctata gtatcaccta attcgtttac acctttgcct aatatcggag
     6781 atacaaagct ttgtattaat aatgaaagag atgcccatat agtaaatgag gttctgttgc
     6841 aaagttgaat ttatagtata attttaaca
//
LOCUS       DALAGD010000266         6633 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.guided.125, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000266 DALAGD010000000
VERSION     DALAGD010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6633
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.guided.125"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            458..1924
                     /gene="msr(A)"
                     /locus_tag="O2701_002908"
     CDS             458..1924
                     /gene="msr(A)"
                     /locus_tag="O2701_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(A)"
                     /protein_id="HCZ4294734.1"
                     /translation="MEQYTIKFNQINHKLTDLRSLNIGHLYAYQFEKIALIGGNGTGK
                     TTLLNMIAQKTKPESGTVETVGEIQYFEQLNMDVENDFNTLDGSLMSELHIPMHTTDS
                     MSGGEKAKYKLANVISNYSPILLLDEPTNHLDKIGKDYLKNILKYYYGTLIIVSHDRA
                     LIDQIADTIWDIQEDGTIRVFKGNYTQYQNQYEQEQLEQQRQYEQYISEKQRLSQASK
                     AKRNQAQQMAQASSKQKNKSIAPDRLSASKQKGTVEKAAQKQAKHIEKRMEHLEEVEK
                     PQSYHEFNFPQNKIYDIHNNYPIIAQNLTLVKGSQKLLTQVRFQIPYGKNIALVGANG
                     VGKTTLLEAIYHQIEGIDCSPKVQMAYYRQLAYEDMRDVSLLQYLMDETDSSESFSRA
                     ILNNLGLNEALDRSCNVLSGGERTKLSLAVLFSTKANMLILDEPTNFLDIKTLEALEM
                     FMNKYPGIILFTSHDTRFVKHVSDKKWELTGQSLHDIT"
     gene            2023..2922
                     /locus_tag="O2701_002909"
     CDS             2023..2922
                     /locus_tag="O2701_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(C) family macrolide 2'-phosphotransferase"
                     /protein_id="HCZ4294735.1"
                     /translation="MTRHNEIIKCAEKYQLHIQPQTISLNESGLDFQVAFGKDKHGVE
                     WVLRLPRRPDVYKRTKPEKQTVDFLQKNVSFEIPKWKVHAKDLIAYPKLTGKPAATID
                     PEIQNYVWEIEHKPLPENFINTLAETLVDLHNIPEENINVQHINIKTIQEIKNDFQRR
                     MNKVKETYGVSDELWNRWKQWLENDELWPRHATMIHGDLHPGHIMVDNQANVTGLIDW
                     TEATHSDPSMDFIGHHRVFDDEGLEQLITAYGKAGGEIWPRMKEHIIELNAVFPMFIA
                     EFAMESGESAYETMALKELGMKE"
     gene            3408..4016
                     /locus_tag="O2701_002910"
     CDS             3408..4016
                     /locus_tag="O2701_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCZ4294736.1"
                     /translation="MIIGYARVSSIDQNLERQLDNLKTFGVEKIFTEKQSGKSVENRP
                     VFQEALNFVRMGDRFVVESIDRLGRNYDEIIETVNYLKEKDVQLMITSLPMMNEVIGN
                     PLLDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQ
                     KRVIYHRVVEMLEEGKAISKIAKEVNITRQTVYRIKHDKGLS"
     gene            4262..4600
                     /locus_tag="O2701_002911"
     CDS             4262..4600
                     /locus_tag="O2701_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294737.1"
                     /translation="MNLIPFIIILIFSCLWTAITNKYLPSETLDKKGQQDRYDERQRK
                     MFIEILAKSFIWIVYCMLFTLVLKFTGLSPSIEGSWFSQYPEIFFIIVALFLMLFNYY
                     TTNKKYTSKG"
     gene            4605..4790
                     /locus_tag="O2701_002912"
     CDS             4605..4790
                     /locus_tag="O2701_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011276570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ4294738.1"
                     /translation="MKNRIREYRKQKGISQETLSKELKVSRQTINAIENNKYDPTLTL
                     AFKLARLFDTTVDELFS"
     gene            complement(5017..5760)
                     /locus_tag="O2701_002913"
     CDS             complement(5017..5760)
                     /locus_tag="O2701_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCZ4294739.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDI
                     EKIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEK
                     QESRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTST
                     NTRQNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPA
                     NLNDNDSDIEEKSEETEPKKGFWSRLFGS"
     gene            6186..>6633
                     /locus_tag="O2701_002914"
     CDS             6186..>6633
                     /locus_tag="O2701_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCZ4294740.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILR
                     DRLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGL
                     NKPNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRKSNVQKFENR"
BASE COUNT         2523 a          946 c         1023 g         2141 t
ORIGIN      
        1 tgttaaaatt atattataaa ttcaactttg caacagaacc gtattttatg agtacactct
       61 aaattaagga gatgtataat caatctaaaa ttgtattttt atacttttgt tatataatct
      121 ttctacttag agatatattg taaaaaaacc aacaaaatgt tatattaaca ttactatttt
      181 atcgatgaag gaggattaaa atgacagctt ctatgagact caaataaacc tttttgaata
      241 caaaactaaa ttaattcaaa aaggcttgtc cgcaatacac actcatagga tgacttatgt
      301 cattttttat ccttcgatag gaataataaa cgctttcaaa tactaaaaca tcataataaa
      361 tttttaagtg tttgaaagtg taatgtgaat gagacgggag tagcctgtct catttttttg
      421 cgtacacgtc ctttaaatat acaaggagtg tataaatatg gaacaatata caattaaatt
      481 taaccaaatc aatcataaat tgacagattt acgatcactt aacatcggtc atctttacgc
      541 ttaccaattt gaaaaaatag cacttattgg gggtaatggt actggcaaaa ccacattact
      601 aaatatgatt gctcaaaaaa caaaaccaga atctggaaca gttgaaacgg ttggcgaaat
      661 tcaatatttt gaacagctta acatggatgt ggaaaatgat tttaacacgt tagacggtag
      721 tttaatgagt gaactccata tacctatgca tacaaccgac agtatgagtg gtggtgaaaa
      781 agcaaaatat aaattagcta atgtcatatc aaattatagt ccgatattac ttttagatga
      841 acctacaaat cacttggata aaattggtaa agattatctg aaaaatattt taaaatatta
      901 ctatggtact ttaattatag taagtcatga tagagcactt atagaccaaa ttgctgacac
      961 aatttgggat atacaagaag atggcacaat aagagtgttt aaaggtaatt acacacagta
     1021 tcaaaatcaa tatgaacaag aacagttaga acaacaacgt caatatgaac agtatataag
     1081 tgaaaaacaa agattgtccc aagccagtaa agctaaacga aatcaagcgc aacaaatggc
     1141 acaagcatca tcaaaacaaa aaaacaaaag tatagcacca gatcgtttaa gtgcatcaaa
     1201 acaaaaaggc acggttgaga aggctgctca aaaacaagct aagcatattg aaaaaagaat
     1261 ggaacatttg gaagaagttg aaaaaccaca aagttatcat gaattcaatt tcccacaaaa
     1321 taaaatttat gatatccata ataattatcc aatcattgca caaaatctaa cattggttaa
     1381 aggaagtcaa aaactgctaa cacaagtacg attccaaata ccatatggca aaaatatagc
     1441 gctcgtaggt gcaaatggtg taggtaagac aactttactt gaagctattt accaccaaat
     1501 agagggaatt gattgttctc ctaaagtgca aatggcatac tatcgtcaac ttgcttatga
     1561 agacatgcgt gacgtttcat tattgcaata tttaatggat gaaacggatt catcagaatc
     1621 attcagtaga gctattttaa ataacttggg tttaaatgaa gcacttgatc gttcttgtaa
     1681 tgttttgagt ggtggggaaa gaacgaaatt atcgttagca gtattatttt caacgaaagc
     1741 gaatatgtta attttggatg aaccaactaa ttttttagat attaaaacat tagaagcatt
     1801 agaaatgttt atgaataaat atcctggaat cattttgttt acatcacatg atacaaggtt
     1861 tgttaaacat gtatcagata aaaaatggga attaacagga caatctcttc atgatataac
     1921 ttaaaaacta tacactaatt tatattgaat attaggttca tttatatcaa tataaaaaat
     1981 acgttcacaa gaaaaatata atagaaataa ggatccaata aaatgactcg acataatgaa
     2041 attattaaat gtgcagaaaa atatcaatta cacatccaac ctcaaacaat ctcattgaat
     2101 gaatcgggac ttgattttca agttgcattt ggaaaagata aacatggagt agaatgggtt
     2161 ttgagactac caagaagacc tgacgtttat aaacgaacaa aacccgaaaa acaaacggta
     2221 gacttcttac agaagaatgt ttcatttgaa ataccgaagt ggaaagtaca tgcaaaagac
     2281 cttattgctt acccaaaact tacaggtaaa cccgcagcca caatagatcc agaaatacaa
     2341 aattatgtat gggaaattga acacaaacca ttaccagaaa actttattaa cacattagct
     2401 gaaacactcg tagatttaca caacatacca gaagaaaaca ttaacgttca gcatataaat
     2461 atcaaaacca tacaagaaat aaaaaatgac tttcaaagaa gaatgaataa agttaaagaa
     2521 acttatggcg tatcagatga attatggaac agatggaaac aatggttaga aaacgacgaa
     2581 ctatggcctc gacatgcgac catgatacat ggggacttac atccaggaca tataatggta
     2641 gataaccaag caaacgtcac aggtctcata gactggactg aagcaaccca ctccgaccca
     2701 tcaatggact ttataggaca ccatcgtgta ttcgacgacg aaggattaga gcaacttata
     2761 acagcatacg gtaaagctgg aggtgaaata tggccacgaa tgaaagagca tataatagaa
     2821 ctcaatgcag tattcccaat gtttatcgct gagtttgcta tggaatcagg agaatcggcg
     2881 tatgaaacga tggcattgaa agagttaggt atgaaagagt agaacttata ttaaaagagg
     2941 cccattttaa agacgaaggc aatcctgatg aaattttaaa actgcgttta attagtgatg
     3001 gtctttggtt ttctataatg tattaaatat ctggacatgg aacaaatgca acaaatggaa
     3061 agaatcgttt tcgaaatttc taactcctta aatcaggagg ataaataaac ggtcattttc
     3121 attgaaagca cttatataaa tttcatcaat tttaatacct accgtaaata catttcataa
     3181 gtattaaaca gatgattaaa atttttagtt aatttaaata tttatgatag agatgacctt
     3241 caaatgaacg gttctgttac aaagaaaaaa tctatattta tttttagtaa gttttaatta
     3301 tagatatgta cactttgagt acaccctaac catacaaaaa gaattgttga aattataaca
     3361 attaaaacgt atgattaggg tgtatattta ttatggagtg aagatgaatg attataggtt
     3421 atgcgagagt atcatcgata gatcaaaatt tagaaagaca attggataat ttaaagacgt
     3481 ttggtgtgga gaaaatcttt acagagaaac agtcgggaaa gtcagtggaa aatagacctg
     3541 tatttcaaga agcactcaat tttgtcagaa tgggagatcg attcgttgtg gaatctattg
     3601 atcgtttagg acgtaactat gatgaaatta ttgaaactgt gaattattta aaagaaaaag
     3661 acgttcagtt aatgattacc agcttaccta tgatgaatga agtaattggc aatccattac
     3721 tggataagtt tatgaaagat ttaatcattc aaatattagc aatggtttca gaacaagaga
     3781 gaaatgaaag taaacgtcga caagcgcaag ggattcaagt tgcgaaggaa aaaggcgtat
     3841 ataaagggcg ccctttactt tattcaccaa atgctaaaga cccacagaaa cgtgttattt
     3901 atcatcgcgt ggtggaaatg ttagaagaag gcaaagcgat tagtaagatt gcgaaagaag
     3961 ttaatatcac aagacaaaca gtttatagaa ttaaacatga taagggatta tcttgataaa
     4021 ttgattgtca atgcaaatga gcgtctctca cagtgtctca aattccataa catattattg
     4081 aattgagatt ttaataatta tacaagaaaa aagagtacga gttcgcagct ttctatatag
     4141 taattgtttt actaataagg tgttttaaaa tatattataa tgtaaaaagt atttgacatt
     4201 tgtatcaaaa atatttacac tcataaatgt caaatacttt ttacatttat gaggtgttat
     4261 aatgaattta attcctttta ttattatttt aattttttct tgtttatgga cggctataac
     4321 taacaaatat ttaccttcag aaacattaga taaaaaagga caacaagatc gatacgatga
     4381 aagacaacgt aaaatgttta ttgaaatact ggctaaaagt tttatttgga ttgtatattg
     4441 tatgttattt acactagtgc ttaaatttac aggtttgtca ccaagtattg aaggatcttg
     4501 gttttctcaa tatccagaaa ttttctttat aattgtagca ctatttctta tgctgtttaa
     4561 ttactatact acaaataaaa aatatacatc taaaggttaa attaatgaaa aatagaatta
     4621 gagaatatag aaaacaaaaa ggaatttctc aagaaacttt aagtaaagaa ttaaaagtgt
     4681 ctaggcaaac tattaatgca atagaaaaca ataaatatga tcctacattg acattagcat
     4741 ttaagttggc aagattgttt gatacaactg tggatgaatt attttcttaa ccattcattt
     4801 atcacaaaaa gagagtgcga attcgcactc tctttttgtg tagtagtact attaaatttt
     4861 gtctcaataa cgaacgttta taacgcaatc atgatgagag tattaataca gttaattatt
     4921 tgaaatactt tagtttacat aatcgatgat taccagaagt tctcactata taaaagacac
     4981 taaaatttta taacttttag tgcctaatca attattttag ctaccaaaca aacgactcca
     5041 aaaccccttt ttaggttccg tttcttcact cttttcttct atatcactat cattgtcatt
     5101 tagattagct ggaacgtctt ctttttttgc ttcttcacct tgtgtacttt cgtcgctttg
     5161 acttccagat atatccttat attgaatctc tgtttcttta gtttcttcct gatctctgtt
     5221 aatatttaca ggattagtag tatatgtttt ttcttgtgca tcaacgtttt gtctagtgtt
     5281 agtagatgta tcaaagggat aattaaggtg tttttctttt tctaattgat tttctaattt
     5341 ttgaatcttt ttattactct ctaatgccag aacttgctga ttttcaagca attttgatct
     5401 ttcttgcaac tcttttctgt aaaattcaat tgttgtttca tgacgacttt cttgtttttc
     5461 aatttgatta tttaaagtat caatctgatt tttaaggatt tctactattt gagcatcgct
     5521 tttcgattca aaattaatat tatccgaccc ttttttttgt ttgtcttcag tagtactttc
     5581 aaaaccatat ttctttttat tttcatttac tctttgaatg attttttcta tatcggtatc
     5641 ttctttaata aacgaagtgt tttcctgttt tattgtctct atattcaatc gcttaatatt
     5701 attaaaaatc gtttgcttac taacccctaa ttcttcactc aactttttca cacttttcat
     5761 atcatcaacc ctttataaaa tgtttataaa cccttgataa aatattataa gcttataatg
     5821 cttaataata aagtgattaa taaataaaaa taaacatcca tcaacctttt tatcaagggt
     5881 ttatgaaagg tttatgatat ttatcaaggg tttataaacc cttgataaag ctatattttc
     5941 ccttttataa gtttatttaa agtgctataa taaacttaat ataacaaaaa ccccaactac
     6001 tggtaatagt cggggtttgg taataaaacg tttagaaact gtttatttat ataattttcg
     6061 atacttgtat tatataatga ataatatctt agtgcaagca cctaaatcat taaataaatg
     6121 taaagctaaa taaaaacgtt ttattacctc ttaccaaact aaaatatgga aagaggtttt
     6181 ttgtcatgca aaatcaatat tttacagtgc aagaaaatta caaagaaaga ttttatcaaa
     6241 tccctaaagt attctttacc agtgaaaatt ataaaaatct aactaatgat atgaaaatag
     6301 cctatgctat cttacgtgat cgcttaaatt tatctattaa aaatagctgg gttgatgaag
     6361 atggtaacat ttactttgtt tattcaaatg aaaagctaat ggaaatctta aattgtaaaa
     6421 aagaaaagtt aacaaaaata aaaaaagggt tagaaaacga tggtctactc attcaaaaac
     6481 gacgtggttt aaataaacca aatattctat atttaatgaa acctattgtc acagaacgtg
     6541 atatttacaa aatagaaaaa gaagaaaacg atgttgaacc ttacggcgag aaggaagttc
     6601 gaaaatcgaa cgtccagaag ttcgaaaatc gaa
//
LOCUS       DALAGD010000267         5170 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.guided.141, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000267 DALAGD010000000
VERSION     DALAGD010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5170
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.guided.141"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            88..687
                     /locus_tag="O2701_002915"
     CDS             88..687
                     /locus_tag="O2701_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCZ4294741.1"
                     /translation="MAFKLPNLPYAYDALEPYIDQRTMEFHHDKHHNTYVTKLNATVE
                     GTELEHQSLADMIANLDKVPEAMRMSVRNNGGGHFNHSLFWEILSPNSEEKGGVIDDI
                     KAQWGTLDEFKNEFANKATTLFGSGWTWLVVNDGKLEIVTTPNQDNPLTEGKTPILLF
                     DVWEHAYYLKYQNKRPDYMTAFWNIVNWKKVDELYQAAK"
     gene            1055..1780
                     /gene="sasD"
                     /locus_tag="O2701_002916"
     CDS             1055..1780
                     /gene="sasD"
                     /locus_tag="O2701_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070002951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasD"
                     /protein_id="HCZ4294742.1"
                     /translation="MKKLATVGSLIVTSTLVFSSMPFQNAHADTTSMNVSNKQSQNVQ
                     NHRPYGGVVPQGMTQAQYTELEKALPQLSAGSNMQDYNMKLYDATQNIADKYNVIITT
                     NVGVFKPHAVRDMNGHALPLTKDGNFYQTNVDANGVNHGGSEMVQNKTGHMSQQGHMN
                     QNTHMNQQPHMQQGHMQSSNHQMMSPKANMHSSNHQMNQSNKKVLPAAGESMTSSILT
                     ASIAALLLVSGLFLAFRRRSTNK"
     gene            complement(1972..2727)
                     /locus_tag="O2701_002917"
     CDS             complement(1972..2727)
                     /locus_tag="O2701_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCZ4294743.1"
                     /translation="MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCAR
                     FDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLARV
                     SMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNHSKQADAFCFTFIPLNVID
                     TFKVNTQNEDDMKTFVEQTIYSNAYQSDLKMSITKAPHFKNHSHVFDGDTHCWLIIET
                     LYAQTPYPIMINKWYIPQEISELTLTRIRQSDY"
     gene            2963..3670
                     /gene="deoD"
                     /locus_tag="O2701_002918"
     CDS             2963..3670
                     /gene="deoD"
                     /locus_tag="O2701_002918"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCZ4294744.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            3677..5029
                     /gene="tet(38)"
                     /locus_tag="O2701_002919"
     CDS             3677..5029
                     /gene="tet(38)"
                     /locus_tag="O2701_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HCZ4294745.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFPLL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVSLNRWTQSEK
                     "
BASE COUNT         1777 a          790 c          909 g         1694 t
ORIGIN      
        1 ggatataaca aataaataat aattattgca aaacacaccc gaaattaatt attataaaag
       61 tatattcata aaaggaggaa tatacttatg gcatttaaat taccaaattt accatatgca
      121 tatgatgcat tggaaccata tatagatcaa agaacaatgg agtttcatca cgacaaacat
      181 cacaatacgt acgtgacgaa attaaacgca acagttgaag gaacagagtt agagcatcaa
      241 tcactagcgg atatgattgc taacttagac aaggtaccgg aagcgatgag gatgtcagtc
      301 cgtaataatg gcggtggtca ttttaaccat tcattattct gggaaatact atcacctaat
      361 tctgaagaaa aaggtggcgt aatagatgac atcaaagcgc agtggggcac tttagatgaa
      421 tttaaaaatg aatttgcaaa taaagcaaca acattatttg gatcaggttg gacttggtta
      481 gttgttaatg atggcaaatt agaaattgtg acaacgccaa accaagataa tccattaaca
      541 gaaggcaaaa caccaatctt actatttgat gtttgggagc atgcctacta tctgaaatat
      601 caaaataaac gtccagacta tatgactgca ttttggaaca ttgttaactg gaaaaaagtt
      661 gatgaattat accaagcagc aaaataatat aacttaatat aattgaggtg gagcatctac
      721 aaggtgttcc acttttttgt acttaatatc tttacttatt tgtttttgtg taatgggata
      781 gcacgtaaaa gtggaaaaat aataagataa atttcaactc gaaatacctt attattgttg
      841 actataacga tgacctaaat attatataca ttagacaaca ctattaatta atcaacatat
      901 tgacattaaa taaattgaca aaataagtaa ttattgtgaa tctaaagtga aatttttata
      961 aaaaaatgta atgattcaaa aattttgttg catttctttt gtaatcgtat gataatgtaa
     1021 atgtaatcaa attgtaatat aaggggacaa gacaatgaaa aaattagcaa cagtaggttc
     1081 tttaattgta acaagcactt tagtattctc aagtatgcct tttcaaaatg cgcatgccga
     1141 cacaacttca atgaatgtgt cgaataaaca aagccaaaat gtacaaaatc atcgtcctta
     1201 tggcggagta gtaccacaag gaatgacgca agcacaatat actgaattag agaaagcttt
     1261 accccaatta agcgctggca gtaatatgca agactataat atgaaattgt atgatgcgac
     1321 gcaaaatatt gctgataaat acaatgtgat aattacaact aatgtagggg tatttaaacc
     1381 acatgctgtt agagatatga atggccatgc gttaccttta acaaaagatg gcaattttta
     1441 tcaaacgaat gtagatgcaa atggtgttaa tcatggtggt agtgaaatgg tgcaaaataa
     1501 aacaggtcat atgagtcaac aaggccatat gaatcagaac acacacatga accaacagcc
     1561 acacatgcaa caaggtcata tgcaatcatc aaaccatcaa atgatgagtc caaaagcaaa
     1621 tatgcattca tcaaatcatc aaatgaacca aagtaacaaa aaagttttac cagctgctgg
     1681 tgaaagtatg acatcaagta ttcttactgc aagtattgcc gcactactat tagtatctgg
     1741 gttattctta gcatttagac gacgttcaac aaataaataa acataatacg attaataata
     1801 gaaaaatcgt gtgattatct gagcgagcct aggacataaa tcaatgtcct aggctcgcta
     1861 atgttatatt ggcagtagtt gactgaatga aattgcgctt gtaacaagct tttccatttc
     1921 ttaccaactc cctaaatagt catcaaaaaa tttcttatat tttaataatt tttaataatc
     1981 cgattgtctt atacgtgtca gtgttaattc agatatttcc tgtggaatat accacttatt
     2041 aatcataatt ggataaggtg tttgtgcgta cagtgtttca ataatcagcc aacaatgtgt
     2101 atcaccatca aacacgtgac tatgattttt gaagtggggc gctttggtaa tagacatttt
     2161 taaatctgat tgatatgcat tgctataaat cgtttgctca acgaatgtct tcatgtcgtc
     2221 ttcgttttgt gtattcactt taaatgtgtc aatgacattt aacggtataa aggtaaagca
     2281 aaatgcatca gcttgcttag aatgattgtc ctttttttga taatagcgtt ccattgcaat
     2341 gacggcagaa ggatggtttg caaacaaatg atttgtatat tcactttcta aatcaacacg
     2401 ataattaatt gatgacatag atacgcgagc tagcaatatt tgatcaagtg gatgcttaaa
     2461 ttgatccata cttgaagcgt gttgggcatt tgtttgtgga ataacaaagt gtcccttccc
     2521 tcttgtactc tctacgatgc catcttcggc taacaatttt atagcttggc gcaaagtcat
     2581 acgactgaca tcaaagcgcg cacaaagttc cttttcagta ggtaatgcat ggccactcgg
     2641 atattttcct atttgaattt ctttatataa cgtattataa atcgttaaaa attttggttg
     2701 tgtttgcgtc acgtagacaa cctccataaa gttacttaat cactctcatc atacaataat
     2761 ttttactcaa attggaaaaa ttataaaaat taaatataga taggctttga aaattagttt
     2821 tatacaaggt tagtagctgt aactgtaaaa tgttcttaat attgtcaaaa tgtaatgctt
     2881 gaaagcgctt ttaaaaaata ttattatata catggttaga caaatagaca aatcactata
     2941 caaatattgg gaggaatatt ttatgaaatc aacaccacac attaaaccaa tgaatgacgt
     3001 cgaaattgca gaaacggttc tattgccagg agatccgtta agagctaagt tcattgcaga
     3061 aacttatttg gatgatgtgg aacagttcaa tacagtgcga aacatgtttg gttttaccgg
     3121 aacatataaa ggtaaaaaag tttctgtcat gggttcaggt atgggtatgc catctattgg
     3181 catttactct tatgaattaa ttcatacatt tggttgtaaa aaattaattc gcgttggctc
     3241 ttgtggcgcg atgcaagaaa acattgattt atatgatgtg attattgcac aaggtgcctc
     3301 tactgattca aattacgttc aacaatatca attaccaggt cattttgcgc caattgcttc
     3361 ttatcaatta ttagaaaaag cagttgaaac agcacgtgac aaaggtgtac gtcatcatgt
     3421 aggtaatgtg ttatcaagtg atattttcta taacgcggat acaacagcga gtgaacgttg
     3481 gatgcgtatg ggtattttag gtgtagaaat ggaatcagct gcattataca tgaatgcaat
     3541 ttacgctggt gtcgaagcat taggtgtgtt cacagtgagc gatcatttaa ttcatgaaac
     3601 gtcaacaaca cctgaggaaa gggaacgtgc atttacagat atgattgaaa ttgcactgtc
     3661 attggtgtag atgattatga atgttgaata ttctaaaata aagaaagcag tacctatttt
     3721 attattctta tttgtattca gtttggttat agacaactca tttaaattga tttctgtagc
     3781 cattgctgat gacttaaaca tatctgtaac gacagtaagt tggcaagcga cattagccgg
     3841 tttagtaatt ggtattggcg ctgtagtata cgcttcatta tctgatgcca ttagtatacg
     3901 cacactattt atttatggcg tgatattaat cattatcgga tcaattattg gttacatttt
     3961 ccaacatcaa ttcccattac ttttagttgg acgtattatt caaactgccg gtttagctgc
     4021 tgcagagaca ttatatgtga tatatgttgc aaagtatctt tctaaagagg accagaagac
     4081 ttaccttggc ttaagtacga gcagttattc cttgtcatta gttatcggta cattatcagg
     4141 tggatttatt tctacgtatt tacactggac aaatatgttt ttaattgcat taatcgtagt
     4201 atttacgttg ccattcctat ttaaattatt accaaaagaa aataatacga ataaagctca
     4261 tttagatttt gttggcttaa ttctagtggc aactattgct acaacagtca tgctgtttat
     4321 tacgaacttt aattggttat atatgattgg tgccttaatt gcgattatcg tttttgcgct
     4381 atatattaaa aatgcgcaac gtccattagt aaataaatca tttttccaaa ataaacgtta
     4441 tgcttcattt ttatttatag tatttgtaat gtatgctatc caattgggtt atatttttac
     4501 gttcccattc ataatggagc aaatttatca tctgcaacta gacacaacat cactgttatt
     4561 agtaccgggt tatatagtag cagtcattgt tggtgcacta agtggtaaaa tcggcgaata
     4621 tctgaattca aaacaagcga ttatcacagc aattatttta atagcactga gcttgatttt
     4681 acctgcattt gcagtaggta atcacatttc aatcttcgtc atttctatga tattctttgc
     4741 aggtagcttt gctttaatgt atgcaccttt acttaacgaa gccattaaaa caatagatct
     4801 taatatgaca ggtgtggcta ttggttttta taatttaatt attaatgtgg cggtatctgt
     4861 aggtattgcg attgctgcgg ctctaatcga ttttaaagca ttaaatttcc caggcaatga
     4921 tgcattaagt tcacatttcg gtattatttt aattatttta ggtttaatga gtattgtcgg
     4981 attagtttta ttcgtcagct taaatcgttg gacacaatct gaaaaataaa tagatataaa
     5041 ttcgcgagat atattcgtat ttatagtaaa attaaataaa gagattatat aacacgagga
     5101 gtagtaagta tgaaatttga gaaatatata gatcacactt tattgaagcc tgagtcaaca
     5161 cgtacgcaaa
//
LOCUS       DALAGD010000268         4655 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.guided.145, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000268 DALAGD010000000
VERSION     DALAGD010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.guided.145"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            354..1310
                     /locus_tag="O2701_002920"
     CDS             354..1310
                     /locus_tag="O2701_002920"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCZ4294746.1"
                     /translation="MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYKGP
                     DMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
                     GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQL
                     YQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLA
                     RESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
     gene            1510..1989
                     /locus_tag="O2701_002921"
     CDS             1510..1989
                     /locus_tag="O2701_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCZ4294747.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            2004..2843
                     /gene="fakB2"
                     /locus_tag="O2701_002922"
     CDS             2004..2843
                     /gene="fakB2"
                     /locus_tag="O2701_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HCZ4294748.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTYNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSIGVAHANVIEYVDKLKKVFNEAFHVNNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            2929..3462
                     /gene="msrA"
                     /locus_tag="O2701_002923"
     CDS             2929..3462
                     /gene="msrA"
                     /locus_tag="O2701_002923"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCZ4294749.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHVDNPTYE
                     QVCTNQTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DEHQKKAAEFKKQQLNEQGIFKKPVITPIKPYKNFYPAEDYHQDYYKKNPVHYYQYQR
                     GSGRKAFIESHWGNQNA"
     gene            3455..3883
                     /gene="msrB"
                     /locus_tag="O2701_002924"
     CDS             3455..3883
                     /gene="msrB"
                     /locus_tag="O2701_002924"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCZ4294750.1"
                     /translation="MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKIS
                     GKPLFTSEEKFHSECGWPSFSKALDDDEIIELVDKSFGMLRTEVRSEESNSHLGHVFN
                     DGPKESGGLRYCINSAAIQFIPYEKLEELGYGDLISHFDK"
     gene            3895..4395
                     /locus_tag="O2701_002925"
     CDS             3895..4395
                     /locus_tag="O2701_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCZ4294751.1"
                     /translation="MFKKLFGKGKEVQKDIAIYAPLTGEFVKIEDIPDPVFAQKMMGE
                     GFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNGLELLVHIGLDTVQLDGEGFEVL
                     VSSGDEVNVGDPLVRFNLEYINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKV
                     IDVTMN"
     gene            4395..4616
                     /locus_tag="O2701_002926"
     CDS             4395..4616
                     /locus_tag="O2701_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HCZ4294752.1"
                     /translation="MKNYSFYQFVMTVRGRHDDKGRLAEEIFDDLAFPKHDDDFNILS
                     DYIETHGDFTLPMSVFDDLYEEYTEWLKF"
BASE COUNT         1701 a          674 c          830 g         1450 t
ORIGIN      
        1 gaatatagtt gtattctatt tgttaaataa agtccgtttt taccaactat attttctaga
       61 aatttaactg ttttaatagg acatcaaaca taatattcaa atcatgtgtt aacctctttt
      121 ttaaaatttt ttagcattaa agttatagat ttgggtaaac aattaccaat tggaaacata
      181 tatcacgtta cgatggggta ggtacttaat cagcatttta taaataaagt aacggaattc
      241 atgatattaa tatcatattc ctaaaatgag tgataacaaa atgctacata aagttaagtt
      301 atatcaaact aaatatacat actataaata atgaaaatga ggtgttatcg catatgttga
      361 attcatttga tgcagcatat cacagtcttt gtgaagaagt tttagaaata ggaaatacac
      421 gaaatgatcg cacaaataca ggtacgattt cgaaatttgg tcatcaactt cgctttgact
      481 tatctaaagg atttccacta ttaacgacaa agaaagtttc ttttaaatta gtagcaaccg
      541 aattattatg gttcattaaa ggagatacaa acatccaata cttattaaaa tataataata
      601 atatatggaa cgaatgggct tttgaaaatt atatcaaatc agacgagtat aaaggtccag
      661 atatgacaga tttcgggcat cgtgcattga gtgatcctga atttaacgaa caatataaag
      721 aacaaatgaa acaatttaag caacgtattc ttgaagatga tacatttgcg aagcaattcg
      781 gggatttagg aaatgtttat ggtaaacaat ggcgagattg ggttgataaa gatggtaatc
      841 attttgatca acttaaaaca gtaattgaac aaattaagca taatccagat tcaaggcgac
      901 acatcgtatc tgcatggaat ccaacagaaa ttgatacaat ggcacttccg ccttgtcata
      961 ccatgttcca gttttatgtc caagatggta agttaagttg ccagttatac caacgtagcg
     1021 cagatatctt tttaggtgtg ccatttaata tcgcaagcta cgctttattg acacacctta
     1081 ttgccaaaga atgtggactt gaagtgggtg aatttgtgca tacatttgga gatgcacata
     1141 tttattcaaa tcatattgat gcgattcaaa cacaattagc acgtgaaagc ttcaatcctc
     1201 caacattaaa aattaacagt gacaagtcta ttttcgacat aaattatgaa gatttggaaa
     1261 ttgttgacta tgaatcacat ccagcaataa aagctccaat agcagtgtag tcattgcata
     1321 gttagctaac catatagaca tcaaaatgac atcatagtat tttcaagtgc aaaaaagtac
     1381 ttttttgtgt taaacgtttt cataaattat gcaaaatcat tatttctatc acactttatg
     1441 ataaaaattg tgttaaatta aagataactt agtaataaaa aatgaaatga tagaagaagg
     1501 aggataatta tgactttatc cattctagtt gcacatgact tgcaacgagt aattggtttt
     1561 gaaaatcaat taccttggca cctaccaaat gatttgaagc atgttaaaaa attatcaaca
     1621 ggtcatactt tagtaatggg tcgtaagaca tttgaatcga ttggtaaacc actaccgaat
     1681 cgtcgaaatg ttgtacttac ttcagataca agtttcaacg tagagggcgt tgatgtaatt
     1741 cactctattg aagatattta ccaactaccg ggccatgttt tcatatttgg agggcaaaca
     1801 ttatttgaag aaatgattga taaagtggac gacatgtata ttactgttat tgaaggtaaa
     1861 ttccgtggtg atacgttctt tccaccttat acatttgaag actgggaagt tgcctcttca
     1921 gttgaaggta aactagatga gaaaaataca attccacata cctttctaca tttaattcgt
     1981 aaaaaataag ggggaaaacg accatgacaa aacagattat agtaacagac tcaacatccg
     2041 atttatctaa agaatactta gaagcaaaca acattcatgt aattccttta agtttaacta
     2101 ttgaaggagc ttcatacgtt gaccaagtag atattacatc agaagaattt attaatcata
     2161 ttgaaaatga tgaagatgta aagacaagtc agccagccat aggtgaattt atatctgctt
     2221 atgaagaact aggaaaagat ggctctgaaa tcataagtat tcatctttct tcaggattaa
     2281 gtggtacata taacactgct taccaagcaa gtcaaatggt agatgctaat gtaactgtta
     2341 ttgattcaaa atctatttct tttggtttag ggtatcaaat acaacaccta gtagagcttg
     2401 taaaagaagg tgtctcaact tctgaaatag ttaaaaagtt aaatcattta agagaaaaca
     2461 ttaaattatt tgtagttata gggcaattga atcaattaat taaaggtggc agaattagta
     2521 aaacaaaagg tttgattggt aatcttatga aaattaaacc aattggtaca ctagatgatg
     2581 gtcgcttaga gcttgtgcac aatgcgagaa ctcaaaattc tagtatccaa tacttgaaaa
     2641 aggaaattgc tgaatttata ggagatcatg aaatcaaatc cattggtgtc gcacatgcta
     2701 acgtcattga atatgttgat aaattgaaga aagtttttaa tgaagctttt catgtgaata
     2761 attacgatat aaatgtaact acaccagtta tttctgcaca tactggtcaa ggtgcgattg
     2821 gcctcgtagt ccttaagaag taaatttaat cttttcagtg ttaattactt ccatttcaat
     2881 cctttataga ctaaatttat aattagatag atagaggagg taattcatat gacaaaagaa
     2941 tatgcaacat tagcaggagg atgtttctgg tgcatggtta aaccatttac atcatatcca
     3001 ggcatcaagt cagtcgtatc tggttatagt ggcggtcatg ttgacaaccc aacttatgaa
     3061 caggtatgta cgaatcaaac cggccatgtc gaagcagtac aaattacgtt tgatccagag
     3121 gttacttcct ttgaaaatat attagacata tatttcaaaa catttgaccc aactgatgat
     3181 caagggcaat ttttcgatag aggcgaaagc tatcaaccag tcattttcta tcatgatgaa
     3241 catcagaaaa aggctgctga gtttaaaaag caacaattaa atgaacaagg tattttcaag
     3301 aaaccagtga ttacacctat taaaccatat aaaaatttct atccagctga agactaccat
     3361 caagattatt acaaaaagaa cccggtacat tattaccaat atcaacgtgg ttcaggtaga
     3421 aaagcgttta tagaatcaca ttgggggaat caaaatgctt aaaaaagata aaagtgaact
     3481 aacagatata gaatatattg ttacacaaga aaacggcact gaaccaccat ttatgaatga
     3541 atattggaat cattttgcta aaggaattta tgtagataaa atttctggta aacctttatt
     3601 tacatctgaa gaaaagtttc attctgaatg tggatggcct agcttttcca aagcgcttga
     3661 tgacgatgaa attatagaat tagtcgacaa atcatttggt atgttgagaa ctgaagtgcg
     3721 ttcagaagaa tcaaatagtc atttaggaca tgtctttaat gatggaccta aagaaagtgg
     3781 cggcttaaga tactgtatca attccgctgc aattcaattt attccatatg aaaagttaga
     3841 agaattgggt tatggcgatt taatatcaca ttttgataag taggagtgga aagtatgttt
     3901 aaaaaattat tcggaaaagg caaagaagtt cagaaagata tcgcaattta tgcaccatta
     3961 actggagaat tcgtgaaaat tgaagatatt ccagatcctg tattcgcaca aaaaatgatg
     4021 ggcgaaggtt ttggtattaa tccaactgaa ggagaagttg tgtctccaat tgcaggacgt
     4081 gttgacaatg tctttccaac taagcatgcg attgggttaa aagcagataa cggattagaa
     4141 ttattagttc atatcggttt agacacagtt caattagacg gtgaaggatt tgaagtgtta
     4201 gtgtctagtg gtgacgaagt taacgtcgga gatccattag taagattcaa ccttgaatat
     4261 attaataata acgctaaatc tgtaatttca ccaattataa ttactaacac tgatcaagca
     4321 gcttcaatta atatttatga tgaaaatgct gtgattaaag gtgaaacaaa agtgattgat
     4381 gtgacaatga actaatgaaa aactattcgt tttatcaatt tgtcatgaca gttcgtggtc
     4441 gacacgacga taaaggtcgt ctagcagaag agatatttga cgatcttgct ttcccaaaac
     4501 acgatgatga ttttaacata ctgtctgatt atattgagac acatggtgat ttcacattgc
     4561 caatgtctgt atttgatgat ttatatgaag aatatacgga atggctaaaa ttttaatata
     4621 atttttaata ataatagtta gaaccagggt gatgc
//
LOCUS       DALAGD010000269         3713 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.guided.163, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000269 DALAGD010000000
VERSION     DALAGD010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.guided.163"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            37..2046
                     /gene="mecA"
                     /locus_tag="O2701_002927"
     CDS             37..2046
                     /gene="mecA"
                     /locus_tag="O2701_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP2a family beta-lactam-resistant peptidoglycan
                     transpeptidase MecA"
                     /protein_id="HCZ4294753.1"
                     /translation="MMKKIKIVPLILIVVVVGFGIYFYASKDKEINNTIDAIEDKNFK
                     QVYKDSSYISKSDNGEVEMTERPIKIYNSLGVKDINIQDRKIKKVSKNKKRVDAQYKI
                     KTNYGNIDRNVQFNFVKEDGMWKLDWDHSVIIPGMQKDQSIHIENLKSERGKILDRNN
                     VELANTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQQMDQNWVQDDTFVPLKTV
                     KKMDEYLSDFAKKFHLTTNETESRNYPLGKATSHLLGYVGPINSEELKQKEYKGYKDD
                     AVIGKKGLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQ
                     KSIYNNMKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEEYNKLTEDKKEPL
                     LNKFQITTSPGSTQKILTAMIGLNNKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVV
                     NGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDIPSDYPFYNAQISNKN
                     LDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKNKVWKKNIISK
                     ENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQIGWFISYDKD
                     NPNMMMAINVKDVQDKGMASYNAKISGKVYDELYENGNKKYDIDE"
     gene            complement(2092..2520)
                     /locus_tag="O2701_002928"
     CDS             complement(2092..2520)
                     /locus_tag="O2701_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HCZ4294754.1"
                     /translation="MKYDDFIVGETFKTKSLHITEEEIIQFATTFDPQYMHIDKEKAE
                     QSRFKGIIASGMHTLSISFKLWVEEGKYGEEVVAGTQMNNVKFIKPVYPGNTLYVIAE
                     ITNKKSIKKENGLVTVSLSTYNENEEIVFKGEVTALINNS"
     gene            complement(2617..3360)
                     /locus_tag="O2701_002929"
     CDS             complement(2617..3360)
                     /locus_tag="O2701_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase"
                     /protein_id="HCZ4294755.1"
                     /translation="MLTVYGHRGLPSKAPENTIASFKAASEVEGINWLELDVAITKDE
                     QLIIIHDDYLERTTNMSGEITELNYDEIKDASAGSWFGEKFKDEHFPTFDDVVKIANE
                     YNMNLNVELKGITGPNGLALSKSMVKQVEEQLTNLNQNQEVLISSFNVVLVKLAEEIM
                     PQYNRAVIFHTTSFREDWRTLLDYCNAKIVNTEDAKLTKAKVKMVKEAGYELNVWTVN
                     KPARANQLANWGVDGIFTDNADKMVHLSQ"
BASE COUNT         1338 a          586 c          566 g         1223 t
ORIGIN      
        1 aatatactac aaatgtagtc ttatataagg agtatattga tgaaaaagat aaaaattgtt
       61 ccacttattt taatagttgt agttgtcggg tttggtatat atttttatgc ttcaaaagat
      121 aaagaaatta ataatactat tgatgcaatt gaagataaaa atttcaaaca agtttataaa
      181 gatagcagtt atatttctaa aagcgataat ggtgaagtag aaatgactga acgtccgata
      241 aaaatatata atagtttagg cgttaaagat ataaacattc aggatcgtaa aataaaaaaa
      301 gtatctaaaa ataaaaaacg agtagatgct caatataaaa ttaaaacaaa ctacggtaac
      361 attgatcgca acgttcaatt taattttgtt aaagaagatg gtatgtggaa gttagattgg
      421 gatcatagcg tcattattcc aggaatgcag aaagaccaaa gcatacatat tgaaaattta
      481 aaatcagaac gtggtaaaat tttagaccga aacaatgtgg aattggccaa tacaggaaca
      541 gcatatgaga taggcatcgt tccaaagaat gtatctaaaa aagattataa agcaatcgct
      601 aaagaactaa gtatttctga agactatatc aaacaacaaa tggatcaaaa ttgggtacaa
      661 gatgatacct tcgttccact taaaaccgtt aaaaaaatgg atgaatattt aagtgatttc
      721 gcaaaaaaat ttcatcttac aactaatgaa acagaaagtc gtaactatcc tctaggaaaa
      781 gcgacttcac atctattagg ttatgttggt cccattaact ctgaagaatt aaaacaaaaa
      841 gaatataaag gctataaaga tgatgcagtt attggtaaaa agggactcga aaaactttac
      901 gataaaaagc tccaacatga agatggctat cgtgtcacaa tcgttgacga taatagcaat
      961 acaatcgcac atacattaat agagaaaaag aaaaaagatg gcaaagatat tcaactaact
     1021 attgatgcta aagttcaaaa gagtatttat aacaacatga aaaatgatta tggctcaggt
     1081 actgctatcc accctcaaac aggtgaatta ttagcacttg taagcacacc ttcatatgac
     1141 gtctatccat ttatgtatgg catgagtaac gaagaatata ataaattaac cgaagataaa
     1201 aaagaacctc tgctcaacaa gttccagatt acaacttcac caggttcaac tcaaaaaata
     1261 ttaacagcaa tgattgggtt aaataacaaa acattagacg ataaaacaag ttataaaatc
     1321 gatggtaaag gttggcaaaa agataaatct tggggtggtt acaacgttac aagatatgaa
     1381 gtggtaaatg gtaatatcga cttaaaacaa gcaatagaat catcagataa cattttcttt
     1441 gctagagtag cactcgaatt aggcagtaag aaatttgaaa aaggcatgaa aaaactaggt
     1501 gttggtgaag atataccaag tgattatcca ttttataatg ctcaaatttc aaacaaaaat
     1561 ttagataatg aaatattatt agctgattca ggttacggac aaggtgaaat actgattaac
     1621 ccagtacaga tcctttcaat ctatagcgca ttagaaaata atggcaatat taacgcacct
     1681 cacttattaa aagacacgaa aaacaaagtt tggaagaaaa atattatttc caaagaaaat
     1741 atcaatctat taactgatgg tatgcaacaa gtcgtaaata aaacacataa agaagatatt
     1801 tatagatctt atgcaaactt aattggcaaa tccggtactg cagaactcaa aatgaaacaa
     1861 ggagaaactg gcagacaaat tgggtggttt atatcatatg ataaagataa tccaaacatg
     1921 atgatggcta ttaatgttaa agatgtacaa gataaaggaa tggctagcta caatgccaaa
     1981 atctcaggta aagtgtatga tgagctatat gagaacggta ataaaaaata cgatatagat
     2041 gaataacaaa acagtgaagc aatccgtaac gatggttgct tcactgtttt attatgaatt
     2101 attaataagt gctgttactt ctcccttaaa tacaatttct tcattttcat tgtatgttga
     2161 aagtgacact gtaacgagtc cattttcttt ttttatggat ttcttatttg taatttcagc
     2221 gataacgtac aatgtattac ctgggtatac aggtttaata aatttaacgt tattcatttg
     2281 tgttcctgct acaacttctt ctccgtattt accttcttct acccataatt taaatgatat
     2341 tgaaagtgta tgcatgccag atgcaatgat acctttaaat ctactttgtt ctgctttttc
     2401 tttatctata tgcatatatt gaggatcaaa agttgttgca aattggataa tttcttcttc
     2461 tgtaatatga aggctttttg ttttgaatgt ttctcctact ataaaatcat cgtatttcat
     2521 atatgtctct ctttcttatt caaattaatt ttttagtatg taacatgtta aaggtaagtc
     2581 taccgtcact gaaacgtaag actcacctct aactttctat tgagacaaat gcaccatttt
     2641 atctgcattg tctgtaaaga taccatcaac tccccaatta gcaagttggt ttgcacgtgc
     2701 tggtttgttt acagtccata cgttcaattc ataacccgct tcttttacca tttttacttt
     2761 tgctttagta agtttggcat cttcagtgtt tactatttta gcattacagt aatctaaaag
     2821 tgttctccag tcttcacgaa acgaagttgt atggaatata actgctctgt tatattgtgg
     2881 catgatttct tctgcaagtt taacaagcac aacattaaag cttgaaatga gcacttcttg
     2941 attctgattt aagtttgtta attgttcttc cacttgctta accatacttt tagaaagtgc
     3001 tagtccattc ggtccagtaa taccttttaa ttctacattt aaattcatat tatattcatt
     3061 tgctattttt actacatcat cgaaagttgg gaaatgttca tctttgaatt tttcaccaaa
     3121 ccaagatcct gcagaagcat ctttaatttc atcataattc aattcagtta tttccccgga
     3181 catatttgta gtccgttcta aataatcatc atgaatgata atcagttgtt catcttttgt
     3241 aattgcaaca tctaactcca accagtttat accttctact tctgaagcag ctttaaatga
     3301 tgcaattgta ttttccggag ctttactagg taatcctcta tgtccatata cagttagcat
     3361 attacctctc cttgcatttt tattttttta attaacgtaa ctgtattatc acattaatcg
     3421 cacttttatt tccattaaaa agagatgaat atcataaata aagaagtcga tagattcgta
     3481 ttgattatgg agttaatcta cgtctcatct catttttaaa aaatcattta tgtcccaagc
     3541 tccattttgt aatcaagtct agtttttctg taccccttat ctgcaatttt acttaggatt
     3601 gcttttaact taccccttat cagcaatttt actgagaact gcttttaacg cacctcttat
     3661 ctgcaatttt gcctagaact gcttttaacg tacctcttat ctgcaatttt act
//
LOCUS       DALAGD010000270         3299 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.guided.171, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000270 DALAGD010000000
VERSION     DALAGD010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.guided.171"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            39..416
                     /locus_tag="O2701_002930"
                     /pseudo
     CDS             39..416
                     /locus_tag="O2701_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            413..1294
                     /locus_tag="O2701_002931"
                     /pseudo
     CDS             413..1294
                     /locus_tag="O2701_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143419.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            1410..2339
                     /locus_tag="O2701_002932"
     CDS             1410..2339
                     /locus_tag="O2701_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4294756.1"
                     /translation="MKNLNNIIEINNLTKQYGSQIAVNNLSITVESGQIYGFLGRNGA
                     GKTTTIRMLLGLIKPTKGNIKIFEKEFESNKTEILKNIGAMVETPGFYSNLTAKENLT
                     INAKILGIQKENAIEEVLEIVGLASEKVKLFKNYSLGMKQRLGIARALLHNPELLILD
                     EPTNGLDPIGIKEIRKLIKDLAQSRNITILISSHILSEIEQLVDKVGIIHNGVLLEEI
                     MYEDLKKKNRQFISISVSDEEKVACLLETNFGIYDYKIKENGEFKIYSHIDRQQEINR
                     LLVLNNIDVFQMRVIEDNLETYFEDLVGGGTIG"
     gene            2332..3069
                     /locus_tag="O2701_002933"
     CDS             2332..3069
                     /locus_tag="O2701_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4294757.1"
                     /translation="MDNLLRTEFIKLKRSYMFLISILGTCVAPFMVVVASFISMRTKV
                     PTPNIVFKQLFEEVSLYHMLIIGPPLYGVIIAYLFGREYIEDTLKNLLIIPISRESFL
                     VSKIILLFLWIQTLSVIAWLLTLMLGMLLHFQGLSQSLIIDSFFTFVIGGVLLWILSP
                     PIIILSIYFKNYVPTIVLTIIITLINLMTANSEHRGLFPWAAVGDISRGTLPNTYPFL
                     LSLIIIIVTSIFSVIILFSYFKKTDIS"
     gene            3083..3286
                     /locus_tag="O2701_002934"
     CDS             3083..3286
                     /locus_tag="O2701_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008406263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4294758.1"
                     /translation="MGFIEQIKAFILGVVEGLTEFAPVSSTGHMIIVDDMWLNTADDL
                     GSASATTFKVVIQLGGSVAKLNL"
BASE COUNT         1201 a          433 c          587 g         1078 t
ORIGIN      
        1 attagtgatt agctatattt tttactttgc aacagaacca gccatttaat atgactgtta
       61 caattactaa gattcaagca ctattacgcc gcacgtatga tttcaccact caagttaata
      121 atttaacagt agatgattgc gagttactac ttgatgcagc aaagcttaaa tttaatgaag
      181 aaaccataga cctgacacac actgaattgc aaatcatgca tagcttattc cagaacaagg
      241 gcaaattcgt cagtcgtaat gcgttaatcg aaaaatgctg ggaatcagaa cacttcattg
      301 atgacaatac gcttgctgtc aacattacac gtttacgcaa aaagctacaa aaactaggac
      361 ttacagattt tatcgaaacg aagaaaaatg tcggatatcg tgtaggtgac gtatgaattt
      421 cttgaaatca atccaacgtg aaatttatat tgtcatcctt acttgtgctt tattctactt
      481 tatattattc atgtataatg tgccgatgca agcatatatg attggtctag gtattgtatt
      541 gtttgtttca atgatttact ggggtatcca ttacatgaac tttaaaaaag aataaacact
      601 acaggaacag aacgaagatt taagaatcga aaaccataaa cttcgtaatg actttattaa
      661 ctaccaaagt gagttagagt catatttttt aatgtgggtg catcaaatca agacaccgat
      721 tacagcatca aagttactgc ttgaagatcc tgatgaacac gtgatttcta acgtgcgtaa
      781 tgaagtctta caaattgata attacacgaa tcttgcactg aactatttga aactcatgaa
      841 ccaaaagaca tacatggtct ttatggaagt gagtgtagat gaattaattc gaccactgat
      901 taaacgttat gcgatacagt ttattcatca tcaaacaaag ttgcattatg agaaaagtga
      961 agcggttata ttaacagatg ccaaatgggc atcaattatg attgagcaaa tattaaacaa
     1021 tgcattaaaa tatgcacgtg gcaaagatgt gtggattacg tttgacgaaa atacaaagaa
     1081 attatcaatc cgagataacg gtatcggcat taatgcgagt gatttaccga aaatctttga
     1141 taaaggtttc tcgggctata acggtagaat aagtgagaaa tcaagcggta ttggtttata
     1201 tattgtcagc acaatctcaa agcgcttagg ccataaagta agtgctacat ctacattaaa
     1261 cgatggttca atattcacca ttcaatttaa ataactccaa tctttcattt ttgtaagatt
     1321 agcggctgtt tttgtaagaa tagataaagg cagccttttc taatcgcgcg taagataaag
     1381 gtaacaacaa actaagaaag gtgccagagg tgaaaaattt gaataatata attgaaataa
     1441 ataacttaac aaagcaatat ggaagtcaga tagcagttaa taatttgagt ataactgttg
     1501 aaagtggaca aatttatggt tttttaggaa gaaatggtgc aggcaaaaca actactataa
     1561 gaatgctttt aggcttaatt aaacctacga aaggtaatat taaaatattt gaaaaagagt
     1621 tcgaaagtaa taaaactgaa attttaaaaa atattggtgc tatggttgaa acgccaggtt
     1681 tttatagtaa tttgacagct aaagagaact taactataaa tgctaaaata ttaggtatac
     1741 aaaaagaaaa tgcaattgaa gaagtgttag aaatagtagg actagcttct gaaaaagtaa
     1801 aattgtttaa aaattattct ttaggtatga agcaacgttt agggattgcg agagctttat
     1861 tgcacaatcc cgaattattg atacttgatg agccgacgaa tggattagat cctattggca
     1921 taaaagaaat tagaaagttg attaaggatc tggctcaatc tagaaatata accattttaa
     1981 tttcaagtca tatattgtca gaaatagagc agttagtaga caaggtgggt attattcata
     2041 acggagtttt gttagaagaa attatgtatg aagatttaaa aaaaaagaac agacaattta
     2101 taagtatatc agtatcagat gaagaaaaag ttgcatgttt gttggaaact aacttcggaa
     2161 tttatgacta taaaattaaa gaaaatggcg aatttaaaat ttattcacat attgatagac
     2221 aacaagaaat taatagatta ttagtattaa acaatattga tgttttccag atgagagtga
     2281 tagaagataa tctagaaact tatttcgaag acttagtagg aggtggcaca attggataat
     2341 ctactccgta cagaatttat taagttaaaa agatcatata tgtttctaat aagtatttta
     2401 ggtacatgcg tagcaccttt tatggtggtt gtagcttctt ttattagtat gcggacgaaa
     2461 gtaccgactc ctaatattgt atttaaacag ttatttgaag aagtcagttt atatcatatg
     2521 ctaattattg gacctcctct atatggggtt ataatagcct atttgtttgg aagagaatat
     2581 atagaagata ctttaaaaaa cttattaatt atacctatat ctagagaaag ttttttagta
     2641 agtaaaataa tattattatt cttgtggatt caaacactat ctgttattgc atggttgcta
     2701 actttaatgc taggtatgct attgcatttt caagggttaa gccagagttt aataattgat
     2761 tcattcttta catttgttat tggcggagtg ttattatgga tattatctcc accaataatt
     2821 attttgtcga tatattttaa aaattatgtt cccacaattg ttttaaccat aataattact
     2881 ttgataaatt taatgactgc taattctgaa catagaggat tatttccttg ggctgcagtt
     2941 ggagacatat ctagaggaac attacctaat acttatccat ttctattatc tttaattatc
     3001 ataattgtta catctatatt cagtgtaatt atattattct catactttaa aaaaactgat
     3061 ataagttaaa aggaggagaa aaatgggttt tatagagcag attaaagcat ttattttagg
     3121 tgtagttgaa gggttgacag aatttgctcc agtatcatca acaggtcata tgattattgt
     3181 tgatgatatg tggttaaaca ctgctgacga tttaggaagt gcgtctgcaa ctacttttaa
     3241 ggtggtaata cagttaggag gttctgttgc aaagttgaat ttataatata attttaaca
//
LOCUS       DALAGD010000271         3167 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.guided.175, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000271 DALAGD010000000
VERSION     DALAGD010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.guided.175"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            122..757
                     /locus_tag="O2701_002935"
                     /pseudo
     CDS             122..757
                     /locus_tag="O2701_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            754..1284
                     /gene="sat4"
                     /locus_tag="O2701_002936"
     CDS             754..1284
                     /gene="sat4"
                     /locus_tag="O2701_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HCZ4294759.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFEKAVFWYLRF"
     gene            1377..2171
                     /locus_tag="O2701_002937"
                     /note="aph(3')-IIIa"
     CDS             1377..2171
                     /locus_tag="O2701_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HCZ4294760.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            2447..3018
                     /locus_tag="O2701_002938"
                     /pseudo
     CDS             2447..3018
                     /locus_tag="O2701_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
BASE COUNT         1065 a          497 c          722 g          883 t
ORIGIN      
        1 attagtgatt agctatattt tttactttgc aacagaacca tcatttttat taaaatactc
       61 ttttgtaaaa tctttctgct cttcaatgta tttaatttct ttacctctaa atctattata
      121 gctatttgat gattacaata aaattgatct taccttattg cccttggaag agttagataa
      181 ttacctaaag ggcgataaat taataaaggt tctaattgat aaagattgta gaattaaaag
      241 ggacatagtt ccgactgata tagattatca tgtaagaaag ccaagcgcaa gggagtatga
      301 tgattgctgc aatgaatttt ggaatgtaac accttatgtt attaaaggat tgtgccgtaa
      361 ggaaatttta tttgctattg atcattttaa tcagattgtt cgccatgagc tgctgagaat
      421 gatatcatgg aaggtcggca tcgaaacagg ctttaaatga agtgtaggca agaactataa
      481 gtttattgaa aggtatatat ccgaggattt gtgggagaaa cttttgtcca cctaccggat
      541 ggattcctat gaaaacatat gggaagcatt atttctatgc catcaattgt tcagggcggt
      601 atccggtgag gtggcggaaa ggcttcatta tgcctatccg gagtatgata ggaatataac
      661 aaaatatacc agggacatgt ataaaaaata cactggtaaa accggctgcc tggatagcac
      721 atatgccgct gatatagaag agaggcggga acagtgatta cagaaatgaa agcagggcac
      781 ctgaaagata tcgataaacc cagcgaacca tttgaggtga taggtaagat tataccgagg
      841 tatgaaaacg agaattggac ctttacagaa ttactctatg aagcgccata tttaaaaagc
      901 taccaagacg aagaggatga agaggatgag gaggcagatt gccttgaata tattgacaat
      961 actgataaga taatatatct ttactaccaa gacgataaat gcgtcggaaa agttaaactg
     1021 cgaaaaaatt ggaaccggta cgcttatata gaagatatcg ccgtatgtaa ggatttcagg
     1081 gggcaaggca taggcagcgc gcttatcaat atatctatag aatgggcaaa gcataaaaac
     1141 ttgcatggac taatgcttga aacccaggac aataacctta tagcttgtaa attctatcat
     1201 aattgtggtt tcaaaatcgg ctccgtcgat actatgttat acgccaactt tgaaaaagct
     1261 gttttctggt atttaaggtt ttagaatgca aggaacagtg aattggagtt cgtcttgtta
     1321 taattagctt cttggggtat ctttaaatac tgtagaaaag aggaaggaaa taataaatgg
     1381 ctaaaatgag aatatcaccg gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag
     1441 atacggaagg aatgtctcct gctaaggtat ataagctggt gggagaaaat gaaaacctat
     1501 atttaaaaat gacggacagc cggtataaag ggaccaccta tgatgtggaa cgggaaaagg
     1561 acatgatgct atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc
     1621 atgatggctg gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt
     1681 atgaagatga acaaagccct gaaaagatta tcgagctgta tgcggagtgc atcaggctct
     1741 ttcactccat cgacatatcg gattgtccct atacgaatag cttagacagc cgcttagccg
     1801 aattggatta cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag
     1861 acactccatt taaagatccg cgcgagctgt atgatttttt aaagacggaa aagcccgaag
     1921 aggaacttgt cttttcccac ggcgacctgg gagacagcaa catctttgtg aaagatggca
     1981 aagtaagtgg ctttattgat cttgggagaa gcggcagggc ggacaagtgg tatgacattg
     2041 ccttctgcgt ccggtcgatc agggaggata tcggggaaga acagtatgtc gagctatttt
     2101 ttgacttact ggggatcaag cctgattggg agaaaataaa atattatatt ttactggatg
     2161 aattgtttta gtacctagat ttagatgtct aaaaagcttt aactacaagc tttttagaca
     2221 tctaatcttt tctgaagtac atccgcaact gtccatactc tgatgtttta tatcttttct
     2281 aaaagttcgc tagatagagt tctatattag agatgtaaag agttttggtg aagaggtttg
     2341 tgagtatggc ttttataacc agggtgcaat gagaagcact aacagcagct agcttaagaa
     2401 cgcataggat atacttttta tggaagtcac agaggaggaa taaaatttga acaggattaa
     2461 cagagtaacc tctattctta ttcagctgca atcaaaaaaa taattcctgc caaagaaatt
     2521 gcacagcgat ttaatataag cttaaggaca gtttacagag atatccggac acttgaagaa
     2581 gccggaatac caattggatc tgaggccgga aaaggatatt ttcttgtaga gggcttccta
     2641 cttctgccgg taatgtttac agcggcggaa gtgggtgcat tgattacagc agggaaattt
     2701 ttaaattgcc atggagatga atcatttata aaggattttg attcagctat gtataaaatc
     2761 aaatctattt tgaagcatgg tgaaaaaaac tatgcacagg agttggagaa cagtattaac
     2821 gtgtacagca catctggaca gaaaaataca ttggcggata acgttattgc agcaattcag
     2881 actgcaatct gcaataaaag ggtaatatca attcaatatc ctgcatcagg agggcaagaa
     2941 ccggaaagca gaatgataga gcctatatca ctggggtttt atgaacagaa ctggtactta
     3001 atcggttttg caggctaaga aatgaatata ggaattttcg tgtggatcgg attaaaagca
     3061 tttgcataga agaagagctc tgtcaaagcc atgatggctc tttggaacaa atattaaaac
     3121 aaatgctggt tctgttgcaa agtaaaaaaa tatagctaac cactaat
//
LOCUS       DALAGD010000272         1549 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8* isolate Sample 189 from
            subject 379 SAMN14154616-rid16265273.guided.208, whole genome
            shotgun sequence.
ACCESSION   DALAGD010000272 DALAGD010000000
VERSION     DALAGD010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154616
            Sequence Read Archive: SRR11139397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525659.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525659.1
            Genome Coverage       :: 317x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 04:02:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 32
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 29 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 18 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154616-rid16265273.guided.208"
                     /strain="ST8*"
                     /isolate="Sample 189 from subject 379"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            87..1073
                     /locus_tag="O2701_002939"
     CDS             87..1073
                     /locus_tag="O2701_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer MecR1"
                     /protein_id="HCZ4294761.1"
                     /translation="MLSSFLMLSIISSLLTICVIFLVRMLYIKYTQNIMSHKIWLLVL
                     VSTLIPLIPFYKISNFTFSKDMMNRNVSDTTSSVSHMLDGQQSSVTKDLAINVNQFET
                     SNITYMILLIWVFGSLLCLFYMIKAFRQIDVIKSSSLESSYLNERLKVCQSKMQFYKK
                     HITISYSSNIDNPMVFGLVKSQIVLPTVVVETMNDKEIEYIILHELSHVKSHDLIFNQ
                     LYVVFKMIFWFNPALYISKTMMDNDCEKVCDRNVLKILNRHEHIRYGESILKCSILKS
                     QHINNVAAQYLLGFNSNIKERVKYIALYDSMPKPNRNKRIVAYIVCSISSFT"
     gene            1045..1305
                     /locus_tag="O2701_002940"
     CDS             1045..1305
                     /locus_tag="O2701_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HCZ4294762.1"
                     /translation="MYVVYRASHETAKEALGDKELRAIAHELTKTVKDNMSVDWSKRD
                     SAKAKMRVQVRRLLKKYGYPPDLQKMAVEQVVEQAELMASQQ"
     gene            complement(1296..>1549)
                     /locus_tag="O2701_002941"
     CDS             complement(1296..>1549)
                     /locus_tag="O2701_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011274780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HCZ4294763.1"
                     /translation="VFGFMKAILGFTRMSVRGLNKVKRELGFVLMALNIRKVVAQRAE
                     NNQKIYKKDNFYIISIEIVFFHLSKNFMSRTHYDLFFYC"
BASE COUNT          558 a          212 c          256 g          523 t
ORIGIN      
        1 atataagact acatttgtag tatattacaa atgtagtatt tatgtcaaaa taatgttata
       61 atttttgtga tatggaggtg tagaaggtgt tatcatcttt tttaatgtta agtataatca
      121 gttcattgct cacgatatgt gtaatttttt tagtgagaat gctctatata aaatatactc
      181 aaaatattat gtcacataag atttggttat tagtgctcgt ctccacgtta attccattaa
      241 taccatttta caaaatatcg aattttacat tttcaaaaga tatgatgaat cgaaatgtat
      301 ctgacacgac ttcttcggtt agtcatatgt tagatggtca acaatcatct gttacgaaag
      361 acttagcaat taatgttaat cagtttgaga cctcaaatat aacgtatatg attcttttga
      421 tatgggtatt tggtagtttg ttgtgcttat tttatatgat taaggcattc cgacaaattg
      481 atgttattaa aagttcgtca ttggaatcgt catatcttaa tgaacgactt aaagtatgtc
      541 aaagtaagat gcagttctac aaaaagcata taacaattag ttatagttca aacattgata
      601 atccgatggt atttggttta gtgaaatccc aaattgtact accaactgtc gtagtcgaaa
      661 ccatgaatga caaagaaatt gaatatatta ttctacatga actatcacat gtgaaaagtc
      721 atgacttaat attcaaccag ctttatgttg tttttaaaat gatattctgg tttaatcctg
      781 cactatatat aagtaaaaca atgatggaca atgactgtga aaaagtatgt gatagaaacg
      841 ttttaaaaat tttgaatcgc catgaacata tacgttatgg tgaatcgata ttaaaatgct
      901 ctattttaaa atctcagcac ataaataatg tggcagcaca atatttacta ggttttaatt
      961 caaatattaa agaacgtgtt aagtatattg cactttatga ttcaatgcct aaacctaatc
     1021 gaaacaagcg tattgttgcg tatattgtat gtagtatatc gagcttcaca tgaaacagct
     1081 aaagaagctt tgggcgataa agagttaaga gccattgcac atgagttaac taaaacagtt
     1141 aaggataaca tgagtgttga ttggtctaaa cgagacagtg ctaaagctaa aatgagagtt
     1201 caagttagac gcctattaaa gaaatatggc tatccaccag atcttcaaaa aatggctgtg
     1261 gaacaagttg tagagcaagc agaattaatg gcaagtcagc aataaaaaaa taaatcataa
     1321 tgagtccggg acataaagtt cttggataag tgaaaaaaga caatttctat tgaaataata
     1381 tagaaattgt cttttttata aattttttga ttattttcag ctcgttgagc tactactttt
     1441 cttatattaa gtgccattaa tacaaaacca agttctcttt tgactttatt gagtcctcgg
     1501 acagacatcc gagtgaaacc caaaatagcc ttcataaatc caaaaacag
//
